LOCUS       DAOQGS010000001        25330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_904_length_25330_cov_5.183501, whole genome shotgun sequence.
ACCESSION   DAOQGS010000001 DAOQGS010000000
VERSION     DAOQGS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 25330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25330
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_25330_cov_5.183501"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PKZ52_00005"
     CDS             complement(<1..866)
                     /locus_tag="PKZ52_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNG58173.1"
                     /translation="MHDLVQNYYGQQLTNSQDLKTSACCDISQVPEWLKPLLAKLHPA
                     VVERYYGCGLVCPSDLSGLRVLDLGCGAGRDVFLLSQLVGEGGEVVGVDMTSEQLAVA
                     RDYQAYHAEQFGFDNVRFVQGYIEQLDQLGLEAGSFDLVVSNCVVNLSPDKPSVLAGV
                     ARLLKPGGELYFSDVYCDRRLPEALRQDPVLYGECLGGALYWNDFTRLAKQAGFNDPR
                     LVEDRPLEITDPVLAERLGEHRFYSATYRLFNLPQLEDACEDYGQAVRYKGTCQQQPH
                     AFVLDKHHRFVAG"
     gene            complement(1017..1667)
                     /locus_tag="PKZ52_00010"
     CDS             complement(1017..1667)
                     /locus_tag="PKZ52_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNG58174.1"
                     /translation="MPRPLQLILFAKAPLAGQAKTRLIPALGLEGSAQLARWLLQHSL
                     KQAEGALQQGFVQFLHLCTNPPLANWPWTPDLAHWQVSCQVEGDLGARMAAPTQSALT
                     QGAVLLMGTDCPQLTPARIGEAARALAEHDAVLIPATDGGYVLLGLNTWLPQLFSQMP
                     WSTSQVAAITCDRLRAAGLSCRLLPPLADVDEPGDLAALPWAAVGGSLSPTNPDLV"
     gene            complement(1715..2404)
                     /locus_tag="PKZ52_00015"
     CDS             complement(1715..2404)
                     /locus_tag="PKZ52_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNG58175.1"
                     /translation="MTPDQLCIVVPLYREAPQLVALWQSLAGLRQAGVEVILVNAELP
                     PERADNTCDIATTQAISLLGAPKGRALQMNTGAQASTRPWLLFLHADTRLPDDVLEVL
                     SRLPDTAQWGRFDVQISGRSWGLAMVAFFMNLRSRLTGIATGDQALFVRRELFLAAGG
                     FPAQPLMEDIALCAHLRKRHRPVCLASRVTTSGRRWDTYGLWPTIVLMWRLRFAYWRG
                     AAPEQLAALYK"
     gene            complement(2401..3213)
                     /locus_tag="PKZ52_00020"
     CDS             complement(2401..3213)
                     /locus_tag="PKZ52_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNG58176.1"
                     /translation="MKTQTKFWLLALLFSLALPLKAEPLINWQPWQALLHQAVQVERG
                     GQASRVDYGYLAGQKTAISQLLIEAAKLDKARFDQASKQEQLAFLINLYNLATVELVL
                     TAYPKLTSIKDLGSLLRSPWQQKRIQLWGASISLDDLEHGLIRNSDRYNDPRIHFAVN
                     CASIGCPALRPEAYSAEQLEAQLEQQARLFLADRSRNRWDGKGLLLSSIFKWYGDDFA
                     KGWRGAKSLSQFLALYGDALGLTPEQTSELSGERVRLEFLPYDWGLNREAGP"
     gene            complement(3218..5413)
                     /locus_tag="PKZ52_00025"
     CDS             complement(3218..5413)
                     /locus_tag="PKZ52_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG58177.1"
                     /translation="MKKSLPLKLLLLATLAGLVATAVYWDLAHWLSFEQVKAHLGFLR
                     AWQQSNPLLFAAGFFGLYVVVTALSLPGAAVMTLAAGALFGLLYGSLLVSFASSIGAS
                     LAFLSARYLAADLVEQRLGRHLAAIHQGVAKDGAFYLFTLRLIPVVPFFAINLLMGLT
                     RMPLWVFYGVSQLGMLAGTLVYVNAGTALGDVQTLSGVFSPALLGSFALLAAFPWLAR
                     AVLARWNRARRYRGFNKPARFDRNLIVIGAGAAGLVTSYIAAALKARVTLVERHQMGG
                     DCLNYGCVPSKALIKSAKLAHQLRHRAQYGLAEDSAEVGQVDFAAVMARVNRVIGEVA
                     PHDSIERYESLGVEVLQGHAQLLDPWRVQITDAEGAKQVLSARSIVLASGASPRVPDI
                     PGLAAVGFWSSETLWHNLSQANQLPQTVLVLGGGPIGCELSQALARLGAKVVQVERGP
                     RLLAREDAEVSELVAKALRADGVELCLEQQVLRFERREGQDGQEQQKWAQLSGQGEHR
                     ALMFDELIIALGRTPRLSGYGLEELGLLDEEGGLVSDSFLASLMPNIYAAGDLVGPYQ
                     FTHVAAHQAWYACVNALFGGIKRFAVDYRVIPATTFVDPEVARVGLNEREAKAQGIAY
                     EVTRFDLEELDRAIADGATEGFVKVLTRPGSDQILGACIVGERAGDLLAEFVLAMRWK
                     LGLNKILGTIHSYPTWAEANKYAAGSWKRAHAPQWALRWLRRYFTWQRG"
     gene            complement(5410..6417)
                     /gene="arsS"
                     /locus_tag="PKZ52_00030"
     CDS             complement(5410..6417)
                     /gene="arsS"
                     /locus_tag="PKZ52_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252219.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNG58178.1"
                     /translation="MRATLPLLNVHQFPAIHRQALNCLQVNLGYKCNQTCLHCHVNAG
                     PTRKEMMTADNLALIPQVLAKRGIKLLDLTGGAPEMHEGFQELVRAARALGVQVIDRC
                     NLTILQEPGFEAMAAFLAEQQVQVVASLPCYASTNVDKQRGAGVFAKSIAALQKLNRL
                     GYGVPGSGLELNLVYNPQGPVLPPEQSKLQRAYQDRLKADFGVVFNQLFTITNMPIAR
                     FGSMLVSKGQFVEYMELLRANHAEANLAQVMCKSLVSVDWQGYLYDCDFNQQLGLVIP
                     GQIASSRRHLRDLLEESLIGAPIRVADHCFGCTAGAGSSCQGALEPAEAQEEAAQALT
                     A"
     gene            complement(6627..7208)
                     /locus_tag="PKZ52_00035"
     CDS             complement(6627..7208)
                     /locus_tag="PKZ52_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNG58179.1"
                     /translation="MVDAAVPALLDADYLVALREQMLRFAVAKLQDAHSAEDVVQEAL
                     MGALLNAQQFAEQSDLRTWVFGILKHKIADAQRRQRRQPQASPLEEPGDDLEAWLERQ
                     PGSAPVLNPAKMAESAQFERVLSGCMGDLSPQQAQVFGLRELNDVDPQAISIERGIGL
                     SYVHVLLHRAKAKLRHCLEHHWLGRPVKPKNIG"
     gene            complement(7303..8403)
                     /locus_tag="PKZ52_00040"
     CDS             complement(7303..8403)
                     /locus_tag="PKZ52_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG58180.1"
                     /translation="MKKHLLLLGAGHAHAVLLRTWAAQGFKPRDCCLISESPLQMYSG
                     MVPGWLAGDYSAQDCQLDVAALCQRLGVEWIAGSVSAIAADARWVEAAGRRFEFDFLS
                     INLGGRQQVQPGGQNSVAIKPFPAFVAQVHQWDTLLTQRARVLPLKLAVVGGGLGGFE
                     LVLGLVARYQTAGPQLSISWFTGPQGPLPKLTAGARSRALQALKGKPVALQQQYFAAD
                     AQGDFAGVVWACQGQAADFLRASGLTLDGAGFIAVDEYFQSLSHGGIFAAGDVCRRLD
                     GTLAPSGVNAVRAGSVLAANLKQRCYGAGHVRGYIPTPWVLQIAALGQHTALAIFGPW
                     YWQAPWVWRLKRFIDRRFMQSFQSPPNTCCGL"
     gene            8877..9866
                     /locus_tag="PKZ52_00045"
     CDS             8877..9866
                     /locus_tag="PKZ52_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58181.1"
                     /translation="MRKFITAGISLIICVISAQANAHVKWFVSDEGAFANVRFSLDTI
                     SGLVVCGAIIFLLLASWAEFNGHSRTQQMLYSPWLKSNYIYALIKASVLILLLGNIIQ
                     GHFIAPNIPIAHHAQTEHVAQALLILLLVFDGVLFAVATLLLCAALILLYGLDIAIDY
                     IFELAAIALAVGLCCPSKTLLALKQKISPAQCQLYALTALRFGLGVQLCVLTVHDKLM
                     HPGLALQFLQEYPYFNFMRLSGLESFSDLHFVLSAGLAEMCFGLLLITNIAQRLASSC
                     ICFFFLLSGLVLGPAELLGHIPIFAAAIILLINPAPSLNLIKKPPLAIKDVRA"
     gene            complement(9943..10884)
                     /locus_tag="PKZ52_00050"
     CDS             complement(9943..10884)
                     /locus_tag="PKZ52_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58182.1"
                     /translation="MADHLNLNDMYIFYVVANAKSITAAGKILHITKQSLSRRVAQYE
                     ESIGVTLISRNTRKLELTQAGAEYLRHCTEVVNQAKIAQSKLQAFQVNAQGSVRIHMP
                     EVFHTPVIAALLAEFSSAHPNLRLDILSCDERSCLIADGIDVQFRLGKLEDCALVARS
                     LGRMDFVQVTSRRAQVDSAEVIPFVYVDGFNYRDFSEPLDLPKSSMQVDNFSLCKKIL
                     LSGYGQSLLPLALCHAELKQGDLINANSHHWQGSKSVNLVFLKNKFLPINVRKFIDFM
                     VEKSRSTIPWQQTITAVQDATKQRTLSTKAALAAPVI"
     gene            11492..11929
                     /locus_tag="PKZ52_00055"
     CDS             11492..11929
                     /locus_tag="PKZ52_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58183.1"
                     /translation="MLPVLCDAITPSLLIALLALPWFYRGGLERRRLVLLSAAQTAVG
                     LTATYSLMAIDNATGWWPRWGMDYSTHSAFALSLALPLVVHHHWAWVFLIGFYGLCMQ
                     LLHYHSWADMLTTALAWAAFCLPIIYAIQQPWVKKRPSTKNTR"
     gene            12049..12432
                     /locus_tag="PKZ52_00060"
     CDS             12049..12432
                     /locus_tag="PKZ52_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58184.1"
                     /translation="MPFIHITSLPPQEPVNMPAVLENISHSFSKFLGLEVKFVTATWN
                     FLPPGHYATGGQAAQFQPLASHPLLVNLVVPGFYRNEQVAKALQATASAIHEVTQIPK
                     TNLFIQAQVAKSGFVFDNGTVVSWN"
     gene            complement(12863..13768)
                     /locus_tag="PKZ52_00065"
     CDS             complement(12863..13768)
                     /locus_tag="PKZ52_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58185.1"
                     /translation="MPSKPNYPKISLDQWAALRAVVEEGSFAKAAERLNKSQSSISYS
                     LGLLNQQLPVPALQLSGRKAELTPAGQVFYRQACQLLDAAMALEEGASRLAQGWEAEV
                     TLALDALVPLQPVLEACCVLARENPATRVRLLETSLSGTDEALLSRQCQLAIMPRVPP
                     GFWPQPLGTIDMVPVVSVKHPLAQGAAIINQDDLRRYRQIVLRDTGIKREQDAGWLGA
                     EQRLTTSHFATSLAAVIAGLGFAFLPAHWVADALIQGQLCLLRLADAEPRRVGLSLVK
                     TDGETAGRATVLLAQVLLERFKQGV"
     gene            13875..14474
                     /locus_tag="PKZ52_00070"
     CDS             13875..14474
                     /locus_tag="PKZ52_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNG58186.1"
                     /translation="MSKQILRLDSSLFGAQGQSTLLNNLVIEGLLARWPDATLVERNL
                     SELPHLDASFFAALGADAQSRSHEQQQQVALADAIIREVQQADVIVLAAPMYNFSVPS
                     QVKSWLDYLARAGTTFRYTAQGVEGLVSNKPVFIQTSRGGVHQGSPRDTIEPLLKSFF
                     SLIGISDLRFTFAEGLNQPQLKEAGWQSAVEQIQKNLSL"
     gene            14507..15355
                     /locus_tag="PKZ52_00075"
     CDS             14507..15355
                     /locus_tag="PKZ52_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNG58187.1"
                     /translation="MSERTVVEMLAAQAASDGDGVRLLRVFGGGNLARFDPFLMLDEF
                     GSESADDYIGGFPPHPHRGFETITYMLQGKMEHRDHLGNQGLLSDGDVQWMTAAHGII
                     HSEMPKQTAGKMRGFQIWLNLPAKNKLAKPAWQDLPASKVPEYAGAGFRIKAIAGSLQ
                     LNELWVEGAFQRPDTRPIYWDIHLEPQRSITIPLPDQFTGLLYTYEGMVNVGAIKVKA
                     RQLARLNDEGELVIHNHTQEEARLLVLAGAKLKEPIVQYGPFVMNTREEIEQAMSDYR
                     DGTLAL"
     gene            complement(15441..16133)
                     /locus_tag="PKZ52_00080"
     CDS             complement(15441..16133)
                     /locus_tag="PKZ52_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HNG58188.1"
                     /translation="MAYFHTELHRGDRAGWLRAAVLGANDGIISLACLILGMAAGQAA
                     TSSILLAALAGVFAGALSMAAGEYVSVQSQADLENADIRKEAAELADYPERELLELKG
                     IYKARGLSSELALEVAKALTAHNALEAHLRDELGIVQETRAAPILASLSSAGSFVLGA
                     SLPCLSLLFSVDYLMPATAAISLLCLLVLGGWGAHMGGAPVLPAASRVLIWGAIAMAI
                     TAGAGFVLGSVV"
     gene            complement(16207..16989)
                     /locus_tag="PKZ52_00085"
     CDS             complement(16207..16989)
                     /locus_tag="PKZ52_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG58189.1"
                     /translation="MESHLPFTLAGLVVGFIVGLTGVGGGSLMTPILLHFGIAPTSAV
                     GTDLLYAAITKSGGIHVHHKKRNIDWGITGWLAAGSLPASLLTLWFLSAGWMDTATLN
                     KVIKVTLGYALVFTALAVLFKTSLVAYAHRRDWWITRLQGEQQRQATIITGAVLGAVV
                     TITSIGAGALGTVALFVLYPMLPTVRLVGTEIAHAVPLTLVAGLGHASVGHVDWTLLG
                     FLLLGSLPGIYIGSHLANRVADRYLRPALSCMLIYVGFKLVL"
     gene            complement(17167..>18509)
                     /locus_tag="PKZ52_00090"
     CDS             complement(17167..>18509)
                     /locus_tag="PKZ52_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58190.1"
                     /translation="LSSSSSSSSSSSSSSSGMAVIQKPVISLVYPIDNANVGSGASVD
                     LDVVVQLADELSGASLYGASVTVEGEAFKQDAADPLLWKGKLTVPKDGITFEIIVETI
                     DKNIFTKTVTYFNKTGALKPLVMGINPGNYILTYDPVAHRIAKVDLINFSWKEYIQNS
                     ALTGTDILFDFNSSYQHAYTMVDGSDANNKRLVAAGVVDGFPPIFFAGSVNNPVNIQY
                     DGKNKRVLVLRKTAENPDKFIAIGVATGNGKADDAIYGGFANGKSEANSFDLITDDLA
                     WDVPLGTTKGPLKAFAFYRNGNMFLIADERIISGVKRTVIQAFSEGSNGTAQWKFEVT
                     LGADISNIAINNGNSSGAAGGFAYVAENKSSLLAGKLKKIDLAKGEVSDLDETKGNVV
                     VANYSELRFDNINQRLYIGDSVADSIYEVNISTRVMRELPVTPAIIDIPPSSEN"
     assembly_gap    18510..18609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18610..19237)
                     /locus_tag="PKZ52_00095"
     CDS             complement(<18610..19237)
                     /locus_tag="PKZ52_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58191.1"
                     /translation="MAPIQSALVKSLLLALVTTSLAACGNGGTSKSKDVKPSSSSSSS
                     SSSSSSSSSSSSSSSTGSTKPSSSSSSSSSSSSSNSSSGNNQQGSEPSSSSSSGGAVV
                     SSSSSSSGAVISSSSSSSSSNSSSSSSSSSNSSSSSSSSSSSNSSSSSGEGASSSSSS
                     SSSSSSSSSSSSSSSSNSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS"
     gene            complement(20193..20348)
                     /locus_tag="PKZ52_00100"
     CDS             complement(20193..20348)
                     /locus_tag="PKZ52_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58192.1"
                     /translation="MPKKLVPISLSQIEEALEERNTKPDRRVTNKPLGDKEERRKRDR
                     RKPAVEH"
     gene            complement(20422..22842)
                     /gene="gyrB"
                     /locus_tag="PKZ52_00105"
     CDS             complement(20422..22842)
                     /gene="gyrB"
                     /locus_tag="PKZ52_00105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNG58193.1"
                     /translation="MAEEPNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDESLAGHCTEIKVTIHPDESITVSDNGRGIPTEMHEEGVSAAEVIMTVLHAG
                     GKFDDNTYKVSGGLHGVGVSVVNALSETLRLTIRRNGQIHEQIYHHGVPQEPLKIIGD
                     TTGRGTTVQFKPSAETFTNILFHYEILAKRLRELSFLNSGVRIVLVDERSGKQDVFEY
                     QGGLKAFVEYLNQNKAAINRIVHFTTQREDGVGVEVALQWNDSFNENILCYTNNIPQR
                     DGGTHLAGFRGALTRCLNNYIESEGIGKKEKIATTGDDAREGLTAIISVKVPDPKFSS
                     QTKDKLVSSEVKTAVEQEMGNIFSAYLLENPSEAKLIVAKMLDAARAREAARKAREMT
                     RRKGALDIAGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQGRDRRTQAILPLKGKI
                     LNVEKARFDKMLSSAEVGTLITALGCGIGKDEYNIDKLRYHRIIIMTDADVDGSHIRT
                     LLLTFFFRQMRELIERGHIYIAQPPLYKISKGKQEQYLKDDEALTQFLTNAAIDSVTL
                     HVNENAPAISGEQLETLVKEFRKGETIVQKHSRVYPTEVLTSLIELPSLTKDQLADQA
                     QVADWTEALGAKLNLISEQSKTHRFVARVFEDLERHLYLPLIEIIAHGVPSQVKISAE
                     FLESTDYKNLRKLGAQLDGLLETGAYVAKGERKHPVANFKEALEWIMAESRKGYNIQR
                     YKGLGEMNPSQLWETTMDPASRRMLKVNIEDAIGADQIFTCLMGDQVEPRRNFIESNA
                     LAVANLDV"
     gene            complement(22854..23951)
                     /gene="recF"
                     /locus_tag="PKZ52_00110"
     CDS             complement(22854..23951)
                     /gene="recF"
                     /locus_tag="PKZ52_00110"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNG58194.1"
                     /translation="MPIQRLAVHQLRNLTGVDIRPCPDLNFFFGNNGSGKTSVLEAIS
                     VLAHGRSFRSHKHKHLINYDCRDFSVFAELDESVGFAKLGVNRGANDVSFKLNGAPVY
                     NSAALANQLPTQVMDAHSFQLLEGPSKLRRQLFDWLVFHVKHDFAALWRDYGRCVKHR
                     NSLLRHDRIARPDLMPWDLELVRLAEAIHQLRSACLLPYLQAVQGLLVEGGLTEELDI
                     QLEYLPGWETDSSLLEQLDQQFGRDKKYGFTTLGAHKSELKITARKMAVHELLSRGQQ
                     KTLVAALYVAQLSVFQAMTDRACVLLIDDVPAELDKQHIAVLGRWVNNLNVQVFATGI
                     ELTSLMEAWPSDTQSIRVFHVKHGEVNEYPV"
     gene            complement(24017..25120)
                     /gene="dnaN"
                     /locus_tag="PKZ52_00115"
     CDS             complement(24017..25120)
                     /gene="dnaN"
                     /locus_tag="PKZ52_00115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNG58195.1"
                     /translation="MRFTISREVLIKPLQLVAGVVERRHTLAVLANILVSVKNGQLSL
                     VGSDAEIEIVAKLPIDSGLELGDTTVPAKKFLDICKSLPDGSTIDFTYDNNKVLVKSG
                     RSKFTLSTLPASDFPLMKAAEDDLAFHCQQKELRRLIDRTGFAMAQQDVRYYLNGMLW
                     EVRADSLRSVATDGHRLAMCTRPLATDVVGLRQAILPRKAVLELSRLLQDSEELAEVA
                     LGDNNIRVSAGGFTFTSKLVDGKFPDYERVLPVGGDKVVVGNRDDFKQAFVRTAILSN
                     DKYRGVRFVVENGVLTITANNPEQEEAEEQVFVDYEGEVLEVGFNVSYLLDVTQVIEA
                     DSMSLTMADNNSSALIQDPINNDSLYVVMPMRL"
     gene            complement(25182..>25330)
                     /locus_tag="PKZ52_00120"
     CDS             complement(25182..>25330)
                     /locus_tag="PKZ52_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG58196.1"
                     /translation="HSLPEIGDLFGGRDHTTVLHACRKIKELLDSSADIREDVKNLLR
                     TLTT"
BASE COUNT         6176 a         6834 c         6329 g         5891 t
ORIGIN      
        1 cccgccacaa agcggtggtg tttatccagc acaaaggcgt ggggctgctg ctggcaggtg
       61 cctttgtagc gcaccgcttg gccgtagtcc tcgcaggcat cctccaactg cggcaggtta
      121 aacaggcggt aggtggcgga ataaaagcga tgttcaccta agcgctccgc caagacgggg
      181 tcggtaatct ccagcgggcg atcttccacc aggcgcgggt cgttaaagcc tgcttgcttg
      241 gctagacggg taaagtcatt ccagtagagg gcgccgccca gacactcgcc gtacaaaacc
      301 gggtcctggc gcagagcctc aggcaggcgc cggtcgcaat acacatcgga gaaatacagc
      361 tcgccgccgg gttttaacag gcgggccacc ccggccaaca cgctgggttt gtccggcgag
      421 aggttcacca cacagttgga taccaccagg tcgaaactgc ccgcctccag gcccagctga
      481 tccagctgct cgatataacc ctgcacaaag cgcacattgt caaaaccgaa ctgctcggcg
      541 tggtaggcct gataatcccg cgccacggcc agctgctcag aggtcatatc cacgcccact
      601 acctcaccgc cctcgcccac cagttgggac agtaaaaaca catcccgccc cgccccgcag
      661 cctagatcca gcaccctaag gccgctcaga tcgctggggc acaccaggcc gcagccgtaa
      721 taacgctcca ctaccgccgg gtgcagcttg gccaacagcg gctttaacca ctcgggcacc
      781 tggctgatat cgcagcaggc gctggttttt aaatcctggc tattggtgag ttgttggccg
      841 tagtagtttt gaaccaggtc gtgcatgggc ggtgtcctcg gtgaatcggg ttgggtgaaa
      901 gcctgagtta gtcgttaaag gcggtgagct tcttacaggg ggtggataat tttttcaccg
      961 gaaattgaat cgaggccctg caaactgggt aggggctacc cctgtggttg cccatttcac
     1021 accaggtcgg gatttgtagg ggaaaggctg ccgccaactg cagcccaggg cagagccgcc
     1081 agatcacccg gctcatccac atctgccaat ggcggcagca accgacaact taagcccgcc
     1141 gcccgcagcc ggtcacaggt aatcgccgcc acttggctgg tgctccaggg catttggctg
     1201 aatagctgag gcagccaggt gttcagcccc agcagcacat aaccgccgtc ggtagcgggg
     1261 attagaactg cgtcgtgctc ggccagggcg cgggcagcct caccgatacg cgcaggcgtc
     1321 agttgcgggc agtcggtgcc catcagtagt acagcaccct gggtcagggc cgattgagtg
     1381 ggggcggcca tgcgcgcacc taggtccccc tccacctggc agctcacctg ccaatgggca
     1441 aggtcagggg tccagggcca gttggccagg ggcgggttgg tgcacaagtg caaaaactgc
     1501 acaaaaccct gctgcaaggc tccttcggcc tgcttaaggc tgtgttgcaa caaccagcgg
     1561 gccaactggg cactgccctc taaacccagg gcggggatca aacgggtttt ggcttggcct
     1621 gccagagggg ctttggcgaa caggattaac tgcaaggggc ggggcatagg gcaaaccaaa
     1681 tgacgtgaag ggttgagggg gaaaccgctg gcagctattt gtagagcgcc gccagctgct
     1741 caggcgctgc gccccgccaa taggcaaaac gcaggcgcca catcaacaca atggtgggcc
     1801 agagaccata ggtatcccaa cgccgcccgg aggtggtgac ccggctggcc aggcacactg
     1861 gccggtggcg tttgcgcagg tgcgcgcaga gggctatgtc ctccatcagg ggttgggccg
     1921 ggaagccccc tgccgccagg aataattccc gccgcacaaa tagggcctga tccccagtgg
     1981 ctatgccggt aaggcgggag cgcaagttca taaaaaaggc caccatagcc aggccccagc
     2041 tgcggccgct gatctgcaca tcaaagcggc cccactgggc cgtgtcaggc aggcgggata
     2101 acacctcaag cacgtcatcc ggcaagcgag tatcggcgtg taggaacaac agccagggcc
     2161 gggttgaggc ttgtgcacct gtattcatct gcagggcccg gcccttgggg gcgcccagca
     2221 gggagatggc ctgggtggtg gcgatatcgc aagtattatc ggctcgttca ggcggcagct
     2281 cggcattcac caaaatcacc tccaccccgg cctgacgcag gcccgccaaa gactgccaaa
     2341 gagccaccaa ctgcggggcc tcccggtaga ggggcaccac aatacacagc tggtcagggg
     2401 tcacggcccg gcctctcggt taaggcccca gtcgtagggc aaaaactcca ggcgcacccg
     2461 ctcccctgaa agctcacttg tttgctcagg ggttaacccc agggcgtcgc cgtagagggc
     2521 taaaaactgg ctgaggcttt tagccccccg ccagcccttg gcaaagtcat cgccgtacca
     2581 tttaaaaata ctggagagca gcaagccctt gccgtcccag cggttgcggc tgcgatcggc
     2641 caaaaacagc cgggcctgct gctcaagctg ggcctccaat tgttctgcgc tgtaggcctc
     2701 tggccgcagg gccgggcagc cgatgctggc gcagttgacg gcaaagtgga tgcgcgggtc
     2761 gttatagcgg tcggaattgc ggatcaggcc gtgttctaaa tcgtccaggc taatgctggc
     2821 gccccagagt tggatgcgtt tttgctgcca gggggagcgc aataaactgc ccaagtcttt
     2881 gatgctggtg agcttggggt aggcggtaag caccagctcc accgtggcga ggttgtacag
     2941 attgattaaa aacgccagtt gctcctgctt gctcgcctgg tcaaagcggg ccttgtcgag
     3001 cttggccgcc tcaataagta actggctaat cgccgttttt tggcccgcca aataaccata
     3061 gtccacccgg ctcgcctggc cgccgcgctc cacctgcacg gcttgatgta acaaagcctg
     3121 ccatggctgc cagttgataa gcggctcggc tttgaggggt agggcgaggc taaacagcag
     3181 ggccagcagc caaaattttg tttgggtttt catcccctta acctcgctgc caagtgaaat
     3241 aacgcctcaa ccagcgcagc gcccattggg gggcgtgggc gcgcttccag ctgccggcgg
     3301 cgtatttatt ggcctccgcc caggtggggt agctgtggat ggtgccaaga atcttgttaa
     3361 gccccagctt ccagcgcatg gccagaacaa actccgccag cagatccccc gcgcgctcac
     3421 ccacaataca ggcacctaaa atctgatcgc tgccgggccg ggtgagtacc tttacaaacc
     3481 cttcggtggc gccatcggca atggcgcgat ccagctcctc aaggtcaaag cgcgtcacct
     3541 cataggcgat gccctgagcc ttggcctccc gctcgtttaa ccccactcgg gccacttccg
     3601 ggtccacaaa agtggtggcg gggatcaccc ggtaatccac cgcaaagcgt ttgatgccgc
     3661 cgaacagggc gttcacacag gcgtaccagg cctggtgggc cgccacatgg gtaaattgat
     3721 aggggccgac caggtccccg gcggcgtaga tattgggcat caggctggca aggaagctgt
     3781 cgctcaccag gccaccctcc tcatctaaca ggcccaactc ctccaaacca taaccgctta
     3841 ggcgcggggt gcggccgagg gcgataatca gctcgtcaaa catcagcgcg cggtgctcgc
     3901 cctggccgct gagctgcgcc cacttctgct gctcttgccc atcttgcccc tctcgccgct
     3961 caaagcgcag cacctgctgc tccaggcaca gctcaacccc gtctgcccgt aacgccttgg
     4021 caaccagctc gctcacctcc gcatcttcac gggctaacag cctggggccg cgctccacct
     4081 gtacaacctt ggcgcccagg cgggcgaggg cctgggataa ctcgcagccg atggggccgc
     4141 cgccgagcac caggactgtt tggggcaact ggtttgcctg gctgaggtta tgccagaggg
     4201 tttcgctgct ccaaaaaccc accgccgcaa ggccgggaat atccggtaca cgggggctag
     4261 ccccgctggc cagtacaatg ctgcgggcac ttaacacctg cttagcgccc tcggcatcgg
     4321 taatttgcac ccgccaaggg tccagcagct gggcatgccc ctgcaacact tcaaccccca
     4381 agctctcata gcgctcgatg ctatcgtggg gggcaacctc gccgataacg cggtttaccc
     4441 gcgccatcac cgccgcaaaa tccacctggc ccacctcggc agaatcttcg gcaaggccgt
     4501 actgcgccct atggcgcagc tggtgggcga gtttggcgct tttaatcagg gctttgctgg
     4561 gcacacagcc gtagtttagg cagtcgcccc ccatctggtg gcgctccact agggtcaccc
     4621 gggccttaag ggcggcggca atgtaggagg tgactaagcc tgcagccccg gcgccaatca
     4681 caattaaatt gcggtcaaag cgggcgggct tgttaaaacc tctgtagcgg cgggcgcggt
     4741 tccagcgggc gagcaccgcg cgggcaagcc agggaaaggc ggccaagagg gcgaaggagc
     4801 ccaacagggc cggggaaaat accccgctaa gagtctgcac atcgccaagg gccgtgcccg
     4861 cattcacata caccagggtg cccgccaaca tgcccagctg actcacccca taaaaaaccc
     4921 acaggggcat acgggtcagg cccatcagta aattaatggc aaaaaacggc accaccggaa
     4981 tcaatcgcag ggtgaacaaa taaaatgcgc cgtctttggc gaccccctgg tgaatggcgg
     5041 ccaggtggcg gcccaagcgc tgttcaacca gatcagcggc taaataacgg gcagataaaa
     5101 acgccaggga ggcaccgata ctggaggcaa acgacaccaa caggctgccg tacaacagcc
     5161 caaacagagc acctgccgcc agggtcatca ccgccgcccc cggcagtgac agggccgtca
     5221 ccacaacata gaggccaaaa aacccagcgg caaacagcag cgggttactc tgctgccagg
     5281 cccgcaaaaa gcctaaatga gccttaacct gctcaaagct cagccaatgg gctaggtccc
     5341 aatacacggc agtggccaca aggcctgcca gagttgccag cagcagtaac tttaacggca
     5401 gggacttctt cacgccgtta gggcctgtgc cgcttcctct tgagcctcag caggctccaa
     5461 ggccccttgg cagctggaac cggcccctgc ggtgcagcca aaacagtggt ccgccactcg
     5521 aatgggcgcg ccgatcaggg actcctccag caaatccctc aagtgcctgc ggctgctggc
     5581 aatttgccca gggattacca ggcccagctg ctggttaaaa tcgcagtcgt acaagtaacc
     5641 ctgccagtcc acactgacta gggatttaca catcacctgg gccaggttcg cctccgcgtg
     5701 gttggccctg agcaattcca tgtattccac aaactggccc ttggacacca acatagagcc
     5761 aaagcgggcg atgggcatat tggtgatggt gaacagctgg ttaaacacca ccccaaaatc
     5821 cgcttttagg cggtcttgat aggctctttg cagcttgctt tgctccggcg gcaacaccgg
     5881 cccttggggg ttgtacacca aattcaactc aaggccggag ccgggcacgc cgtagcctaa
     5941 gcggttgagt ttttgcagcg ctgcaatgct tttagcaaac accccggcgc cgcgctgttt
     6001 atccacattg gtggaggcat aacagggcag ggaggccacc acctgcactt gctgctcagc
     6061 caaaaacgcc gccatggctt caaagcccgg ctcctgcaaa atggtcaggt tacagcggtc
     6121 gatcacctgc acacccaagg cgcgggcggc gcgcaccagc tcttgaaaac cctcgtgcat
     6181 ctccggcgcg ccaccggtta agtccaaaag cttaatgccg cgcttggcca acacctgcgg
     6241 gatcagcgct aggttgtccg ccgtcatcat ctctttgcgg gtggggcctg cgttgacgtg
     6301 gcagtgcagg caggtttggt tgcacttata gcccaggttc acctgcaggc agttaagtgc
     6361 ctgtcggtgg atggcgggga actggtgaac attcaatagg ggtagggtgg cacgcatgac
     6421 aaatctccgc tcaatcgacg aacaaactcg cacccagagg cggccttagc ggctgaatgt
     6481 tgggcaggtg acgagtccgg gataaaaacc tggggcagct tagtcgttat tcgggggtgt
     6541 tttcttacag ctaaattgtg tcagcgaagg atttattcgc cgcctgctga aaatggctat
     6601 tggggccaaa gccgcattta gggcactcag ccaatatttt taggcttaac agggcggccc
     6661 agccagtgat gctctaaaca gtggcgcagc ttcgccttcg ctcgatgcaa caacacatgc
     6721 acgtaactaa gcccaatgcc gcgctcgatg ctgatggctt gagggtccac atcgtttaat
     6781 tcgcgcaatc caaacacctg ggcctgctgg gggcttaaat cccccatgca accgctcagc
     6841 acccgctcaa attgggcgct ttcggccatc ttcgccgggt ttaataccgg cgcactgccc
     6901 ggctgccgct ccaaccacgc ctctaagtca tcgccgggct cctccagggg gctcgcctgt
     6961 ggttggcggc gctggcggcg ttgggcatcg gcaattttgt gtttgagaat gccaaacacc
     7021 caggtgcgca ggtccgactg ctcggcaaac tgctgggcat tcagcaaggc gcccatcaac
     7081 gcctcttgca ctacatcctc cgccgagtgg gcatcctgca gtttcgccac tgcaaaacgc
     7141 agcatctgct cgcgcagggc gactaggtag tcagcgtcta acagcgcagg cacagcggca
     7201 tcaaccataa aaacatctca aattatcggc gcaattcagt agccggctac cctaccccat
     7261 ctaaccggtt agataaaccc tgccgtggcg catcacccag tgctaaagtc cacagcaggt
     7321 gtttggcggc gactgaaaac tctgcataaa gcgcctatca ataaagcgct ttaaccgcca
     7381 cacccagggc gcctgccaat accaggggcc gaagatggcg agcgcagtgt gctggccgag
     7441 ggcggcaatc tgcagcaccc aaggggtagg tatataccct cgcacatggc ccgcgccgta
     7501 gcaccgctgt tttaagttag ccgccagtac gctaccagct cgcaccgcat ttacacccga
     7561 aggtgctagt gtgccgtcca aacgtcggca cacatcccca gcggcaaaaa tgccgccgtg
     7621 ggagaggctc tggaaatact catccaccgc aataaacccc gccccatcaa gcgtaagccc
     7681 actggcgcgc aaaaaatccg ccgcctgccc ctggcaggcc cacaccactc cggcaaaatc
     7741 accttgagca tccgccgcaa aatactgctg ctgtaatgcc acgggcttac cttttagcgc
     7801 ctgtaaagcc cggctgcgcg cgcctgctgt caatttgggc agcggccctt gcgggccggt
     7861 aaaccagctg atgctaagct gcggcccggc cgtttggtaa cgggcaacta aacccaatac
     7921 caattcaaaa ccgcccaagc caccgcctac tacagccaac tttaagggta aaaccctggc
     7981 ccgctgcgtt aacagggtgt cccactggtg cacctgagcc acaaacgctg ggaagggttt
     8041 gatggcgaca gaattctgcc cacccggctg cacctgctgc ctaccaccta aattgatgga
     8101 caaaaaatca aattcaaaac gcctacctgc agcctccacc cagcgcgcat ccgccgctat
     8161 ggcacttaca ctgcctgcaa tccactccac acccaggcgt tggcacaggg ctgccacatc
     8221 cagctgacaa tcctgcgcgc tgtaatcccc cgccaaccag cctggcacca tgcccgaata
     8281 catctgcaat ggcgattcac tgattaaaca acaatccctc ggcttaaagc cctgcgccgc
     8341 ccaggttcgc agcagaacgg catgggcatg gccggcgccg agtaataaca agtgtttttt
     8401 catttaatac tctgtgtgat ggatacttat ttttgctctg taactcccgg cctacagaca
     8461 caatcagaaa cttgcaaaaa cctactttaa aaacacacac cgccccgtac ggaatgcagc
     8521 atttaattta cacagcgtcc tacaaaatgc gcttgtcaaa attacggcta tcgcagctaa
     8581 tgacaaggta catttaaccg cataaataac aggttatttt taatttaatt tcaatatggt
     8641 tgcagcgtta aaaattcatc aagggaacct ctgaaaaaca taggcggcga attttcggag
     8701 attccctaac agcttgttga aatactacct tgcctgagca tcggcgccta tgtgtttcaa
     8761 caaccggctg aggtttgatt gagatcaaaa ctgataatga gaccctatca ggtgcgtcaa
     8821 gtctaatacc aaacgcgcta tatcttgttt taaatataca tacccacgga ggtcatatgc
     8881 gcaaattcat tacagccggt atcagcctaa taatatgtgt tataagtgcg caggccaacg
     8941 ctcacgttaa atggttcgta agcgatgaag gcgcctttgc caatgtgcgc ttctcgctgg
     9001 atactatcag tgggctggtg gtgtgcggtg caatcatatt ccttttactg gctagttggg
     9061 ccgagtttaa cggccatagt cgcacccagc agatgctcta ttcgccatgg ttaaaaagca
     9121 attatatcta cgcactgata aaagcctcgg tgcttatttt attactcggc aatattattc
     9181 aaggccattt tattgcacct aatattccta ttgctcatca cgcgcaaaca gagcatgttg
     9241 cgcaagcatt actgattctt ttgctggtat ttgatggggt tttatttgca gtggcaacac
     9301 tactgttatg cgcagcttta atactgctgt atggccttga tatcgccata gactatatat
     9361 ttgaattggc tgccatcgcc ttggcggtag gtttgtgttg cccatccaaa accttattgg
     9421 cactaaaaca aaaaatttcc ccagcacagt gccagctgta tgcacttact gcgttgcgct
     9481 ttggtttggg cgtgcaactg tgtgtgctga cggtccacga taaattaatg catcccggcc
     9541 ttgcgctaca attcttacag gaataccctt attttaattt catgcgttta agcgggcttg
     9601 aatcctttag cgatttgcat tttgtgctca gcgcagggct tgctgaaatg tgttttggtc
     9661 tgttattaat cacgaacata gcgcagcgct tagcatccag ctgtatttgt tttttctttt
     9721 tgttatcggg tttagtgctg gggcccgctg agttactggg ccatatccct attttcgccg
     9781 ccgctatcat tttattgatc aacccagcgc catcactcaa cctaattaaa aagccgccat
     9841 tagctatcaa agatgttcgc gcttaacaag ctgttgagat agatgggcca acgccctcga
     9901 atgtactcct gggttgttga caatagtaac aatcaatatt cgctaaataa caggcgcggc
     9961 caatgcggct ttagtcgata atgtccgctg ttttgtggcg tcttgaacgg ctgtgatagt
    10021 ttgttgccaa gggatagtgg agcggctctt ttcaaccata aaatctataa atttgcgcac
    10081 attgataggt aaaaatttat tttttaaaaa taccaaatta acacttttgc tgccctgcca
    10141 atggtgtgaa ttcgcattta tcaaatcgcc ttgctttagt tctgcatggc acagcgctag
    10201 gggtaataga ctttgcccat agccgctcaa caatattttt ttacacaatg aaaaattatc
    10261 cacctgcatg gatgatttag gtaaatccaa gggttcagaa aaatctcgat aattaaatcc
    10321 atcaacgtat acaaaaggaa taacctctgc gctatccact tgggcgcgcc tggaggtgac
    10381 ttgcacaaaa tccatgcggc ccaatgagcg cgccaccaaa gcacaatctt ccagcttgcc
    10441 taaacgaaat tgcacgtcta tgccatcggc tattaaacag gatcgctcat cgcacgagag
    10501 tatatccaaa cgcaaattag ggtgggccga ggaaaactct gctaataacg cagcgatcac
    10561 tggggtgtga aacacctccg gcatgtgtat gcgcacgctg ccctgggcgt taacctgaaa
    10621 tgcctgcaat tttgattgtg caattttcgc ctgattgacc acttccgtgc agtgccttaa
    10681 atactcagca ccggcctgtg ttaattctaa cttgcgagtg ttgcgcgaga ttagggttac
    10741 accaatagat tcttcatact gagcgacacg acggcttaaa gactgcttag taatatgtaa
    10801 aattttaccc gccgccgtta tgctcttagc attagcaact acatagaaaa tatacatatc
    10861 gtttaaattt aaatgatccg ccataacaca atctcaataa aaaactggga aatcaacctt
    10921 aagggtgccc gaattaagtc ggtacccacg aatattttga aaccaccccg agcgagtacc
    10981 gacaggcata agccttacta tttaaactac agggcgcgcg cgtttcaaat acgtgtaggt
    11041 tttcataata ttacgcctac aggctcatta gcccgacata aaaatccgcc aggtaataat
    11101 cagaggcttt ttatttattt gatcgaataa catttttcat atctgtgaaa tattcacagc
    11161 ctatacagga ccgcgaacat taataaggct atccaaatag gccgctgata attacctatg
    11221 gagccattag tacggaatgt tttcaccttc tttaaagtga atgccaacgc cagaactaca
    11281 aacctactct caggaaatac tcacaaatca gctattattt acattaatat ccaaacgcgt
    11341 aatcactcac ttacacagct taattttttg cacaatacaa tttgcttatg ttaacagcat
    11401 cattatttcc aacccgccag cataaaacat gcattcatgc tgacactttg tcagaataag
    11461 gtttacaaat actctccaag gagcaggccc tatgctgcca gtactatgtg acgccatcac
    11521 cccaagcctg ttaatagccc tgctcgcgct tccctggttt tatcgcgggg gcttagagcg
    11581 ccgcaggttg gtccttttaa gcgcggcgca aaccgctgtg ggcctcactg caacttacag
    11641 cctgatggct attgataacg ccacgggctg gtggcctcgc tggggaatgg attacagcac
    11701 ccactcggcc tttgcattga gcctggctct gccgctggtt gtacatcacc attgggcatg
    11761 ggttttttta attgggtttt atggtttgtg tatgcagctg ctgcactacc actcttgggc
    11821 cgacatgctg acgaccgctc tcgcctgggc agccttctgc ctaccgatca tttatgcaat
    11881 acaacagccc tgggttaaaa agcgcccatc cacaaagaat actagatagc ccagtggcag
    11941 caatttacac ttagcgccat ccgtcagcgg gtgaggccca tgggcctgcc catctgcccc
    12001 actttatgca cccgcccaaa atcggcggcg ctaacctaag gagccgctat gccctttatc
    12061 catatcacct cgctgccccc ccaggaaccg gtgaacatgc ctgcggtgct ggaaaacatc
    12121 agccatagtt tttctaaatt tctgggtcta gaagtgaaat ttgtcaccgc tacttggaat
    12181 tttttgcccc ccggccacta tgccaccggc ggccaggcgg cacagtttca gcccttagct
    12241 tcccacccct tgttggtcaa cttggtggta cccggttttt accgcaacga acaggtggcc
    12301 aaagccctgc aagccaccgc ctccgctatt cacgaagtga cacaaatccc taaaaccaat
    12361 ctgtttatac aggcccaagt ggccaagtcg ggttttgtgt tcgacaacgg cacagtggtc
    12421 tcctggaact agcccttata gccttattta ccctgtcgat tcaatttaca agccctttgg
    12481 gggacttttg ctggattgca gcagcgaggc tctgtcttca aggtttaatc aggaaaacac
    12541 tcgacggttt tttttacata gcgccgtttt gcaatgcaaa aatcacttat atgtatttga
    12601 ttgtaaataa aaaataattt atggaatgtt gttggctaaa gcgaactacc tattactagg
    12661 ccgtcggtac attaaatgac cggcgaagtg tttaacccaa ctcgacacca aggagattta
    12721 cccatgtcac tgtatttcgt tcttttactt agctgcggct tgctcagcat cgcagcactg
    12781 cgtcgcagac acgtttaatg tattgagcac ctctaattca aaaagcggct atttaagccg
    12841 cttttttttt gtcctgaatt ttctacaccc cctgtttaaa acgctccagc aatacctggg
    12901 ccaacaatac agtggccctg cctgccgtct caccatcggt tttcaccagt gataagccca
    12961 ctcggcgggg ttcagcatcc gcaagcctta acaaacacag ctgtccctgg atcaaggcat
    13021 cggccaccca atgggcgggt aaaaacgcga accccaggcc tgctatcacc gctgccaagc
    13081 tggtggcaaa gtggctggtg gttaaacgct gttcagcccc caaccaaccg gcgtcttgct
    13141 cccgcttgat gccagtatcg cgcaagacaa tttgtcgata acggcgcaaa tcatcctggt
    13201 taataattgc tgcaccctgc gctaagggat gcttcacact caccaccggc accatgtcga
    13261 tagtccccag gggttggggc caaaaccccg gcggcacccg cggcataatg gcgagctggc
    13321 actggcgcga taaaagcgcc tcatctgtgc cggataagct ggtctccagc aagcgcacgc
    13381 gggtggcggg attttcccgc gccaatacac agcaagcctc taacaccggc tgcaggggca
    13441 ccagggcatc taaggccaga gtaacttcag cctcccagcc ctgcgccaac cggctggctc
    13501 cttcttcaag ggccatagcg gcatccaaca gttggcacgc ctggcgataa aacacctgcc
    13561 cggcaggggt taactccgct ttgcggccac ttaactgcag agcaggcacg ggcagttgtt
    13621 ggttgagtag gcccaggctg tagctgatgc tggactggct tttgtttaat cgctcagccg
    13681 ccttagcaaa gctgccttcc tccaccaccg cgcgcagggc tgcccactgg tcgagggaaa
    13741 ttttggggta attgggtttc gagggcatga gttaatacat ctaaatttta gatatttatt
    13801 agcgcaaaat tgcgacaata aatcaattaa ttaaacctat gataaatcca tcaacccaca
    13861 gtgaggattt catcatgagc aaacaaattc ttcgtctcga cagcagccta ttcggcgcac
    13921 aaggccaatc caccctttta aacaatttgg ttatcgaagg cctgctagcc cgctggcccg
    13981 acgctacctt ggtagagcgc aacctaagtg aactacccca tttagatgcg agtttttttg
    14041 ccgccctagg cgccgatgcg caaagccgca gccatgaaca acagcagcaa gtggcccttg
    14101 ccgatgccat tatccgcgaa gtacaacagg cggatgtcat agtacttgcc gcccccatgt
    14161 acaatttctc agtaccctct caggttaagt cctggttgga ttatctggcc cgggctggta
    14221 ctacctttcg ctacacggca cagggggtag aaggtttagt gagcaataaa cctgtattta
    14281 tccaaaccag ccgcggcggc gtacaccagg gctcaccccg cgacacaatc gagcctttgt
    14341 taaaaagttt tttcagctta atcggtatta gcgacctgcg ttttactttt gccgaaggcc
    14401 tcaatcagcc acagttaaaa gaggccggct ggcaaagtgc cgtcgagcaa atacagaaaa
    14461 acttaagcct ataaaccctt tacccacata tgaagaggag atcaccatga gcgagcgcac
    14521 tgtagtagaa atgcttgcag cccaggctgc atcagacggc gacggcgtaa ggttgctgcg
    14581 cgtgtttggc ggcggcaatt tagctcgctt cgatccgttt ttaatgctcg acgagtttgg
    14641 atctgaatcc gccgacgact acatcggcgg cttcccgccc cacccacacc gaggctttga
    14701 aaccatcacc tacatgctgc aaggcaagat ggagcaccgg gatcacttgg gcaaccaggg
    14761 tttattaagc gacggcgatg tgcagtggat gacagcggcc cacggcatca tccacagcga
    14821 aatgcccaag caaaccgccg ggaaaatgcg cggctttcag atttggttga atcttcccgc
    14881 gaaaaacaaa ctggctaaac cggcctggca ggacctaccg gcctctaaag tacccgaata
    14941 tgcaggggcg ggttttcgta tcaaggccat cgcaggcagc ttgcagttaa acgagctttg
    15001 ggtggagggc gcattccagc gccccgatac acggcccatt tattgggata ttcacctgga
    15061 gccgcagcgt tcaatcacta ttcctctacc cgatcaattc accgggttac tctataccta
    15121 tgagggaatg gtaaatgtgg gcgcaattaa ggtgaaggcc cgccaattgg caaggctcaa
    15181 tgacgaaggc gagctggtta ttcacaacca cactcaggaa gaagcccgtt tactggtgct
    15241 ggccggagca aagttgaagg aacccatagt gcaatatggg ccttttgtga tgaatacccg
    15301 cgaggagatc gaacaggcca tgagcgatta tcgcgacggc accttagctc tttaaccaaa
    15361 tagcccttta agcaaaaagc caacctcaac aacctcagtt gaacccctgg caaaagggct
    15421 atttaactgc ggtttttagg ttaaaccaca ctgccgagga caaatccggc tcccgctgta
    15481 atcgccatgg cgattgcgcc ccaaatcaat accctgcttg ccgcaggtaa tacaggggca
    15541 ccccccatgt gtgcacccca tccacccaaa accaataaac acaacaaact aatggctgca
    15601 gtggcaggca ttaaataatc aacggaaaat aacaggctca aacagggcaa gctggctccc
    15661 aaaacaaagc ttcccgccga ggataaagac gctaaaatcg gcgccgcgcg ggtctcttgg
    15721 actatgccta attcatcgcg cagatgggct tcaagtgcat tgtgggccgt taacgccttg
    15781 gccacttcca acgccagctc gctgctcaac ccccgcgctt tataaatacc cttcagctct
    15841 aataattcgc gttcaggata atcagccaac tcagccgctt ctttgcgaat atcagcgttt
    15901 tccaaatccg cctgggactg caccgacaca tactcaccgg ccgccataga cagagcacca
    15961 gcaaacacac ctgctaaagc tgctaagagt atcgatgaag tcgccgcctg gcccgcagcc
    16021 atgcccaaaa tcaaacaggc cagcgagata atgccgtcgt tagcacccaa cacggctgcg
    16081 cgcagccaac cggcgcgatc gccacggtgc aattcagtgt gaaaataagc cattaatccc
    16141 cccaactcac aaaataaaag acgattttat actctgtgga ttaaggggtg gttaaaactg
    16201 gcagtactag agaacgagct taaaacccac ataaatcaac atgcaggaca aagcaggccg
    16261 caggtagcga tcagcgactc gattagctaa atggctgccg atataaatgc caggtagtga
    16321 gccgagcaac aaaaacccca gtagcgtcca atccacatgg ccaaccgaag catggcccaa
    16381 acctgcaacc aaagttaggg gcaccgcgtg ggcaatttca gtccccacca aacgaaccgt
    16441 tggcaacatg gggtagagca caaacagcgc aaccgtgcct aaggcgccgg ctccaataga
    16501 cgtgatagtt accaccgcac caagaacagc gccggtgata atcgtagcct gccgctgttg
    16561 ctcgccctgc aaacgcgtaa tccaccaatc acggcggtgg gcataggcga ctaaagacgt
    16621 tttaaataaa actgccagag cagtaaatac aagggcataa cctagcgtga ccttgatgac
    16681 cttgttgagc gtcgcagtat ccatccaacc ggcagataaa aaccacagag tcagtaaaga
    16741 cgccggtaaa gaacccgccg ctaaccaacc ggttatgccc cagtcaatat tgcgcttttt
    16801 atggtgtaca tggatgccgc cggatttagt gatggccgca taaagtaagt ccgtacccac
    16861 cgcactagtg ggggcgatgc caaaatgcaa caggatcggc gtcatcagag atcccccgcc
    16921 aaccccagta agcccaacaa taaaccctac aaccaaacct gcgagggtga acggtagatg
    16981 tgattccata taaaaaccaa gtgtttaatt ttgaacagag taacagcctg gttataaaag
    17041 gcgaagtaac caaaagttag ttaattattc agaatggtta tatatgtatc ggtcgaacag
    17101 tcctcgcagc tcatgccccc cagaataaaa aaggccagta gtaaactggc ccttggggta
    17161 ctgcttttaa ttctcgctgg aaggtggaat atctataatt gccggtgtta cgggcagctc
    17221 gcgcattacc cttgtggata tatttacctc ataaatacta tcggcaactg aatcacctat
    17281 atacagacgc tggttaatgt tgtcgaaacg caattcagag taattggcaa ctactacatt
    17341 tcctttggtt tcgtccagat cactcacttc acctttagct aaatcaattt tcttaagctt
    17401 accggctaac aaagaacttt tattctccgc tacataggca aaaccaccgg ctgcacctga
    17461 tgaattacca ttattgatag caatatttga tatgtcagct cccaacgtaa cttcaaattt
    17521 ccattgcgca gtgccattgc ttccttcact aaatgcttga ataaccgttc gtttcacccc
    17581 actaataatg cgctcatcag ctattaagaa catattgcca tttcgataaa aagcgaacgc
    17641 ctttaatggc cccttggttg ttcctaaagg tacatcccaa gctaaatcat cagtaattag
    17701 atcaaaagaa ttagcctctg atttaccatt agcaaatcca ccataaatgg cgtcatcagc
    17761 cttaccgtta cctgttgcca cgcctatagc aataaattta tcaggatttt cagccgtttt
    17821 tctcagtact aatacgcgtt tatttttacc atcgtattga atatttacag gattgtttac
    17881 actaccagca aagaatatag gcggaaaccc atctacaacg cctgccgcaa ctaaccgctt
    17941 gttgttagca tcactaccat caaccatggt gtatgcgtgt tggtaactgg aattaaaatc
    18001 aaacaaaata tcggtgcctg tcagcgcact attctgaata tattctttcc aagagaaatt
    18061 aattagatca actttagcaa ttctgtgcgc aacaggatca taagttaata tgtaattacc
    18121 agggttaatt cccattacta gcggtttaag tgctccggtt ttattaaagt aggtgaccgt
    18181 tttggtaaaa atatttttat caatagtctc tacaataatt tcgaaggtaa ttccatcttt
    18241 aggcacagta agcttacctt tccacaacaa tggatcagca gcgtcttgtt taaatgcttc
    18301 gccttcaact gtcacgctag cgccgtacaa ggaagctccc gacagctcat ctgccaattg
    18361 aacgaccaca tctaagtcta cacttgcacc actacccaca ttggcattgt caatcggata
    18421 taccagtgaa ataactggtt tttgtattac tgccattccg ctactagatg agctagaact
    18481 gctagacgaa cttgagctac tggacaagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnt agaactgcta gacgagctag aactgctaga cgagctagaa ctgctagacg
    18661 agctagaact gctagacgag cttgagctac tggacgaact agaactgtta gacgagctag
    18721 agctgctaga tgaacttgag ctactggacg agctagaact actagatgag ctagatgcgc
    18781 cttctccact gctagatgaa gaattcgagc tactgctcga cgacgagctt gagctgctat
    18841 tcgaagaaga acttgaagac gaagatgagt ttgaggagct agacgaagac gatgaactta
    18901 ttactgcacc cgaggatgaa ctgctggagc tgacaacagc accaccgctg gacgagctag
    18961 agctaggttc cgaaccctgt tggttgtttc cagacgatga gttgctactg gaactggagg
    19021 agctgctgga actgcttgag gagggtttgg tgcttcctgt ggagctgctg gagcttgatg
    19081 agctcgaact tgaacttgaa cttgaacttg aacttgaact tgacgatggt tttacatcct
    19141 tagactttga agtaccacca ttaccacacg cggcaagtga tgtagtaaca agcgccaata
    19201 gcagagattt aacaagagca gattgaatag gtgccatttt cccctcgggg tatttttggg
    19261 tttgagcaga tgcgggccaa ggcccctaaa agagcctgac aatgtattga tatgcactag
    19321 agggtttaag gctttacccg gtccgggcaa taagatcttt aacttttata acaataatat
    19381 tggaaaaaat acatcaatag aacaaatcaa caataatgag caaattttaa ccgattattt
    19441 cctttcacag tatccttcta tgtcgtaatt agccgttttg ggagctggct cccaattctt
    19501 gtttaatcct ctgttctatc agttttattt gagtgtttat tgatcaattg aaattgagct
    19561 ctgctttcaa aacaaactga agcaacaaaa caaaatataa aaaaacatat atagaagaga
    19621 atgattttaa tcttgtagtg attattagcc tgccccaggc ttgtgtataa gtttaaaaaa
    19681 tacatttaaa tcaatatatt agcaaaatga taacgcaccg gtttaccact ggaattcatc
    19741 gtaataaccc ccctttgcac ctgtgggtgg tttcttaagg gttaggtaga ttaggtttta
    19801 aacccgccaa tctgtgcata aatacgaggc ttctctacag gttggccaaa agatatgcat
    19861 aagctataaa actaacggcc tcgatctgta taaatatgtt gatagggtgt attaaatctg
    19921 tgggctgatg gggtgtgtgg ataagcttct taataagttg tggtttatgg gttcttttga
    19981 ggtgattatg cggtgaataa tttatttatc cgtttgtcgt caccttaagc cttacgggct
    20041 taaatccgga gattgcccgt gttagccctt gttggacaga agttgagggt tagttgctga
    20101 agtacttggg ggcgctgata gtcagtcaat agccaaacta cagacgaaaa aaaagcagcg
    20161 ttaccgctgc tttttgattt tgaggggcgc cattagtgct ctacggcggg ctttcggcgg
    20221 tcccgcttac ggcgctcttc tttgtcgcct aagggcttat tggtcactcg gcgatcgggt
    20281 ttagtattgc gctcttccag agcttcttca atttgactga gggatatggg aactaacttc
    20341 tttggcatag gagtatcctt taatcgtgtt acagaatgct caaattgcta accgccaact
    20401 aaagcatcca gtaattattg atcaaacatc caaatttgcc actgccaagg cgttggattc
    20461 gataaaattt cgacgtggtt ccacttggtc acccattaag caggtaaata tctggtctgc
    20521 gcctatggca tcttcaatgt ttaccttcag catacggcgt gaggcgggat ccatggtggt
    20581 ttcccacaat tggctggggt tcatttcccc tagtccttta tagcgctgga tgttatagcc
    20641 tttgcggctt tctgccatta tccattctaa ggcttcttta aaattggcta ctgggtgttt
    20701 gcgttcgccc ttggctacat aggcccccgt ctccaataaa ccatccaatt gtgcgccgag
    20761 tttgcgcagg tttttgtaat cggttgattc taaaaactct gcactgattt ttacctggga
    20821 aggcacgccg tgggcgatga tctcgatcag cggcaaatac aggtggcgct caaggtcttc
    20881 aaacacacgt gcgacaaagc ggtgagtttt ggattgctca gaaataaggt taagtttggc
    20941 accgagggct tctgtccaat cggccacctg ggcctgatcg gctagctgat ccttagttaa
    21001 actgggtagc tctatcaggc tggttaacac ctcggttgga taaacccggc tgtgtttttg
    21061 cactatggtt tcacccttgc ggaattcttt gaccagtgtt tcgagctgtt cgccgctaat
    21121 ggcgggggcg ttttcattga cgtgtagggt taccgaatca atagcggcat tggttaaaaa
    21181 ctgtgtgagc gcttcgtcgt ctttcaaata ctgctcttgt ttgcctttgc tgattttata
    21241 caggggcggt tgagcgatat aaatatggcc gcgctcgatg agctcgcgca tttgacggaa
    21301 gaaaaaagtc agcaacaatg tgcggatatg ggagccgtcc acgtcggcgt ccgtcatgat
    21361 gatgatgcgg tggtagcgca atttatcgat gttgtattcg tctttgccaa tgccgcagcc
    21421 cagagcggta ataagcgtgc ctacttccgc gctggagagc attttgtcga agcgcgcttt
    21481 ttccacattc aaaattttac ctttcaacgg caaaatcgcc tgagtgcgac gatcacgccc
    21541 ttgtttggcg gagccgcccg ccgagtcccc ttccactagg tagagttcag acagggatgg
    21601 gtctttttct tggcaatccg ccagtttgcc gggcaaacct gcgatatcta aagcgccctt
    21661 gcggcgggtc atttcacggg ccttgcgagc cgcttcacgg gcgcgggcgg catcgagcat
    21721 tttggccaca atcaattttg cttcagaggg gttttctaat aaataggcgc tgaagatatt
    21781 gcccatctcc tgctccacag cggtttttac ttcggaggag acaagtttgt ctttggtttg
    21841 ggaggagaat ttagggtccg gtactttcac ggaaataatc gcggtcaagc cttcgcgggc
    21901 gtcgtctcct gtggtagcga ttttttcttt tttacctatg ccttctgact caatgtagtt
    21961 gtttaagcag cgggtgagcg cgccgcgaaa accggctaag tgggtaccgc cgtcccgctg
    22021 ggggatattg ttggtatagc agaggatgtt ttcattaaag gaatcgttcc actgcagggc
    22081 cacttcgacc cccacgccat cttcgcgctg ggtagtgaag tggacgatac ggttgatcgc
    22141 ggctttgttt tgatttaggt attcaacgaa ggctttgagg ccgccttggt attcaaacac
    22201 atcttgtttt cctgagcgct catctaccag cactatgcga acgccggagt ttaagaacga
    22261 cagctcccta agccgcttag ccagaatctc atagtgaaac agtatatttg tgaaggtttc
    22321 agcggagggt ttgaactgca ccgtggtgcc gcgaccggtg gtatcaccaa ttattttaag
    22381 cggttcctgg ggaacaccgt ggtggtagat ttgctcatgg atttggccgt tgcggcggat
    22441 ggtcaggcgc agggtttctg ataaagcgtt tactaccgag acgcccacgc cgtgtaagcc
    22501 acctgagact ttataggtgt tgtcatcaaa cttaccgccc gcgtgcaata cagtcatgat
    22561 cacttcagcg gcagatacac cctcctcgtg catttcggtg ggtatgcccc ggccgttatc
    22621 acttacggtg atggattcgt ctgggtgaat ggtgacttta atttccgtac agtggcccgc
    22681 taaggattca tcaatggagt tatcgactac ctcgaacacc atgtggtgca agccggtgcc
    22741 atcgtcagtg tccccaatat acataccggg ccgcttgcgt acagcgtcca agcctttaag
    22801 gactttaata ctcgacgaat cgtaattggg ttcttcggcc atgggtgtct ccattagaca
    22861 ggatattcgt taacttcacc gtgtttcacg tgaaacactc ttattgattg tgtatcgctt
    22921 ggccaggcct ccatgaggct ggtcaattca atgccggtag caaagacctg gacattcagg
    22981 ttgttaaccc agcgcccaag cacggcaata tgttgtttat ccagctccgc aggcacatcg
    23041 tcgatgagta atacacaggc tcgatcggtc atggcttgaa atacactcag ttgcgccacg
    23101 tacaaagcag cgactaaagt cttctgctgc ccacggctta atagttcatg gacagccatt
    23161 ttgcgggccg tgattttcag ttcactttta tgggctccaa gagtagtgaa gccgtatttt
    23221 ttatctcgcc caaactgctg gtccagttgc tctagcaggc tgctatctgt ctcccagccg
    23281 ggtagatatt caagctgaat atccagctcc tctgttaacc caccttctac taataacccc
    23341 tgcactgctt gtagataggg taataggcag gccgagcgca actgatgaat agcctctgct
    23401 aggcgtacca gctctaggtc ccagggcatt aaatcagggc gggcaattct atcatgccgc
    23461 agcaggctat tgcggtgttt aacgcagcga ccgtagtccc gccaaagtgc ggcaaagtca
    23521 tgtttcacgt gaaacaccag ccagtcgaac agttgcctgc gcaatttgct ggggccttcc
    23581 aggagttgaa aggaatgggc atccatgact tgtgtaggca gctggttagc tagggcggct
    23641 gagttatata ccggggcacc gttgagttta aagctcacgt cattggcgcc acggttaacc
    23701 cccagtttgg cgaaaccaac ggattcgtct aattcggcaa ataccgagaa atcacggcaa
    23761 tcgtagttga tcagatgctt gtgtttgtgg gagcggaagg aacggccgtg ggctaaaaca
    23821 cttatggctt ccagcacgct ggttttaccc gagccattat taccaaagaa aaaattaagg
    23881 tcggggcagg ggcggatatc cacccctgtg aggttgcgca attggtgtac ggcgagacgt
    23941 tgaatgggca tgggcaggtg ggaatctaaa gaacagccgt tgaacggggc aaaccccgtt
    24001 cagtgagtcg tttagattaa agccgcatgg gcatcacaac atagagggag tcgttgttga
    24061 tggggtcttg aatcagtgcg ctggagttat tgtccgccat ggtgaggctc atggagtcag
    24121 cctcgataac ctgggtgaca tccaacaggt agctgacatt aaagccaact tctaaaacct
    24181 cgccttcata atctacaaat acttgttctt cggcttcttc ctgctcgggg ttgttggcgg
    24241 tgatagtaag aaccccgttt tcaactacaa agcgcacacc gcggtattta tcgttactca
    24301 gaatagccgt gcgcacaaac gcctgtttga aatcgtctcg gttgcccacc acaaccttat
    24361 caccgcccac cggcagtact cgttcgtagt cggggaattt accgtccacc aatttagagg
    24421 taaaggtgaa accaccggcg gaaacgcgga tgttgttatc gccaagggcg acttctgcca
    24481 gctcctcact gtcttgtaac aggcggctaa gctccagcac agctttgcgc ggcaggatcg
    24541 cctggcgtaa tccaactacg tcggtggcca aggggcgggt gcacatagcg aggcgatggc
    24601 catcggtggc aaccgagcgc aaactgtcgg cgcgaacttc ccataacatg ccgtttaggt
    24661 aatagcgcac gtcctgttgg gccatggcaa aaccagtgcg gtcgatcaag cggcgtaact
    24721 ctttttgttg gcagtgaaaa gccagatcgt cctcggccgc tttcatcaat gggaagtctg
    24781 atgccggtag ggttgaaagg gtaaatttac tgcggccaga tttcaccaac accttgttgt
    24841 tgtcgtaggt gaaatcaatg gtggaaccgt ccggcaggct tttgcagata tccaaaaact
    24901 tttttgcagg tacggtggta tcgcccagtt caagcccact atcaataggc agctttgcca
    24961 caatttctat ttctgcatcg ctgcccacta aggacaattg accgtttttt accgatacca
    25021 atatgttggc gagcaccgca agtgtatggc ggcgttcgac aaccccagcg acgagctgca
    25081 ggggtttgat taacacttct cgggagatag taaaacgcat ggcaggttct cttttattga
    25141 agccagttta gcgatggcct gttagttgtt atgagtggga attaggttgt tagggttcta
    25201 agtaagtttt ttacatcttc acgaatgtcc gccgaggagt ccaacagctc cttaatcttc
    25261 cggcaggcgt gcaatacagt ggtatggtct ctgccgccga ataagtcgcc aatctcaggc
    25321 aggctgtgat
//
LOCUS       DAOQGS010000002        24387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_951_length_24387_cov_4.904365, whole genome shotgun sequence.
ACCESSION   DAOQGS010000002 DAOQGS010000000
VERSION     DAOQGS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 24387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24387
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_951_length_24387_cov_4.904365"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKZ52_00125"
     CDS             <1..835
                     /locus_tag="PKZ52_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNG58197.1"
                     /translation="LHRLIAAAPQIHWLSDSLQDLTREGELCLLHTAAGATIQTPLIL
                     AADGARSAVRSLAAIPVRQWSYGHTALVCRVHTERPHRHTAYQRFTHQGPLAFLPLFP
                     AGQQDSSIVWSLDNTLAEQMMALSEDEFKTALAAALEHRLGAVLQCDARLALPLHQCH
                     ARRYCAPGVTLLGDAAHAIHPLAGQGVNLGLADVAALVDELARAHQRGLPLAHPSVQD
                     RYQRARMPHNLAAMAAMESFKRLFGNSNPYVGLVRNLGLKLAGQLPGVKQQFMRLASG
                     R"
     gene            835..2124
                     /locus_tag="PKZ52_00130"
     CDS             835..2124
                     /locus_tag="PKZ52_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HNG58198.1"
                     /translation="MALGPAKTLVSQQTWQICPHCGELLRRVPACEEQALLCPNCQLP
                     LPAAKTAWVQPATALSVAAVVLFIISNSFDFLSLKLVSFEQSTSILAGVGALLAHQQW
                     LLAALVFSTIFLFPLLEVAALLYLLVPFQLKRRLPGQVAVLRWLIRAQPWSMLEIFLL
                     AILVTSVKLGDMASLIPGVSMYAFFALVPILAIAYGVIDRQSLWSYLQPDNCYVAEPS
                     DLLYACGVCEAMVGETLLNHSAHCPRCLSPMHKRIPNSLQKTTALIVASVILYIPANL
                     LPFMTTRTLTGERDDTLVSGVISLLNHDMWLIAAVVFIASIVVPMAKLGVLIYLLASA
                     HWQHYRNQATKIRLYHLTELIGRWSMVDVYVVTLLTALVQFGFLGNIQPGNALVAFAA
                     VVVLTMLAAETFDPRLLDTPQEQPGDFPAHAFKHEST"
     gene            2111..3736
                     /locus_tag="PKZ52_00135"
     CDS             2111..3736
                     /locus_tag="PKZ52_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNG58199.1"
                     /translation="MNLPKPMVTPFQKLSPVWLIPLVALIIALWLAINSWQEKGQEIE
                     ILLNAAAGIEVGKTQVRLKDVPVGKVSKMQLTADLSKVRLKVTLEREIAKHLSVNTRF
                     WLVSPRISVAGVSNLGTLVSGVYIVMDPGEPGAFSTNFDGLPEPPAIESSVKGTTFLL
                     RADSLDSVDIGSPVYYRQLKVGEVTSYKLSETGEAVEIRVFVQAPHDKLITSHSRFWN
                     VSGFDINLGPDGVKAQMASLVSFLGGGISFENINAYENAEPAKANQTFFLYADKESVL
                     EGRFSAKFYYLLKFSHSVRGLKAGAPVEFRGIKVGEVVDVVLSSIENQDKSLLVYITL
                     EPERFKADLEPKREIIDGMVAKMVDQGLRGQLRTDSLLTGSLYVDLAFENKQKPGKLT
                     VTDNHAEIPTLDDPLDQLTKQLSEITDKFNRIPSDKIGADLAKSMASLANIMNTLDKN
                     NTAGKLDGTLGNLEKATHDMDKTVAEATQTLSQLRVTLKSLDATFAPDSQLHFEVIEM
                     LNSVTEASDSFDRIADELKRYPNALIFGGKKKE"
     gene            3733..4308
                     /locus_tag="PKZ52_00140"
     CDS             3733..4308
                     /locus_tag="PKZ52_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HNG58200.1"
                     /translation="MIARGLLFSLLLWGLCACSQQTHYYTLHHTKGPKAPLGSYTFSL
                     GIGPVFLPESHNQPGVVSLTEGQEVTVAQYHIWAGYLNQSVTRVLADNLSLLLGADTV
                     WPFPWDNRMRPVRQVRVIIEEMAGQRGKTVALQAKYILTADNGERTLAAERTRIEVPC
                     AGDSYEAYVAAINQALNQLSEQMAKTLIAKQ"
     gene            4373..4714
                     /locus_tag="PKZ52_00145"
     CDS             4373..4714
                     /locus_tag="PKZ52_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58201.1"
                     /translation="MKILYVENHPYFGKIVKDKFLSEFHVTIVPSIKSATEAVNQSSF
                     DIALVDYDLDDGKGDEFIAYLIRAGIDLPCIAVSSHENGNSALVKAGAVSVCSKMHFS
                     KIASVVGKINV"
     gene            4823..5050
                     /locus_tag="PKZ52_00150"
     CDS             4823..5050
                     /locus_tag="PKZ52_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58202.1"
                     /translation="MTRALNRILIIPLLLSAYGCDSRSQPQRELTRPAAEVKAVIPSA
                     IDAAAPGAAQAVSAEDIKQAQEAAADSPQHE"
     gene            5142..5579
                     /locus_tag="PKZ52_00155"
     CDS             5142..5579
                     /locus_tag="PKZ52_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58203.1"
                     /translation="MRLKKCVMVLGLAAFLQSTTGCGLLLHPERQGSKGGNLDPVIVL
                     LDGLGLFFFLVPGIVAFAVDVSTGCIYTGGGGGGGKRRHFSSIEQGQPINADELAGMK
                     KIALNGPVTKASIEAALERELGQKVDITAANVQVQPILEPAKI"
     gene            5641..6096
                     /locus_tag="PKZ52_00160"
     CDS             5641..6096
                     /locus_tag="PKZ52_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNG58204.1"
                     /translation="MKKYVFLFVLACTGLLSACNPLAKFQQPQVQLSNLVVLERHGFE
                     QLFLLKLKVTNPNKMALPIAGLQYQVRLNGFDVFTGNAGDLPQLKAYSDTYIELPVSA
                     NLISAGAVLASLLVSEHPSINYQLNATFDLTGVLPDFRVSEKGLVNLEH"
     gene            6247..6621
                     /locus_tag="PKZ52_00165"
     CDS             6247..6621
                     /locus_tag="PKZ52_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58205.1"
                     /translation="MNTLFDFPFRLFDQSISPVTSWFSPNYEINFAGDKNLEQKIIAD
                     VASYGTQLNRITEALLVLAEGRDEPAIQELRKVHENIEQVKTQTKAQLVSRIERDLAK
                     LKAQDAKAYQQLLATLKPGDAE"
     gene            6796..8895
                     /gene="ppk1"
                     /locus_tag="PKZ52_00170"
     CDS             6796..8895
                     /gene="ppk1"
                     /locus_tag="PKZ52_00170"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNG58206.1"
                     /translation="MSEMPDLSQEPAGLESEGHNFDAPRYYLNRELSHLYFNQRVLAQ
                     ALDESHPLLERLNFLLIFSSNLDEFFEIRVAGLIQSIKYQREMSGLDGMQAKESLKLI
                     SEICHRIVDHQYQILNDVLVPELQKQGIRFIRRNQWSAEHSAWVEEYFQKELVPVLSP
                     IGLDSAHPFPRLVNKSLNFIVSLAGKDAFGRDLGLAIVPAPRSLPRFIPLPPHLNSGN
                     DFVFLSSIIHAHTGKLFPGMTVKGCYQFRITRNADLDVDIEGAADLARALRGELHSRR
                     FGTAVRIEVADNCPKEITDYLLRETGLTDDDLYRVNGPVNLKRLMRLPMMVNRPDLRF
                     PPLIPSIPKGFHRKDNIFEAISKKDYLLLHPFESFAPVVQFLRQAAKDPQVVAIKQTL
                     YRTGNDSAIVDALVDAARSGTEVTVVVELRARFDEEENLELASRLQEAGAVVVYGVVG
                     YKTHAKMILVVRREHNQYKRYCHLGTGNYHAVNAHLYTDYSLLTADDMIGEDVHRIFH
                     QLTGMGKTERMQKLFHAPFTLKRQLVKFIDNEIKAAQEGKPARIIFKCNGLTEPKIIH
                     SLYKASQAGVKIDLIVRGMCCLKSGIEGISDNIHVYSIVGRFLEHARVYYFANSGVYC
                     ASADLMERNLNRRVEVCFPIEDQALSARVLAELALYLNDGKQRWVQLESGQYQRVEQD
                     GPAAQMELMRLLTSPST"
     gene            9336..9644
                     /locus_tag="PKZ52_00175"
     CDS             9336..9644
                     /locus_tag="PKZ52_00175"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HNG58207.1"
                     /translation="MKRVLMTGVILLAANSYADTLRCGAKLISTGDSKGSVENICGAP
                     AGTDSYCKPTKSTNSTSSKEQDCVRVDIWRYNRGAGQLGAEVEFQMGEVTAIRNTERE
                     "
     gene            complement(9749..10654)
                     /locus_tag="PKZ52_00180"
     CDS             complement(9749..10654)
                     /locus_tag="PKZ52_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG58208.1"
                     /translation="MTPFVNPFAMNAELTPEQISRILQGINIPPQPQVMVDLQMEQCS
                     PNCSIDTIAHLISQDVGLSGSILKTVNSPFFKHSNKITSIHQAVNLLGVNSVVNLVNA
                     QSIRGALSDDDIIALGRFWDSAMDVAMTSAAIAKHIGFPYPDEAYTLGLFHNCGIPLL
                     MVRFKNYLEVQIEAYANPEQAITTTENHLIRTNHAVVGYYVGKSWNLPRYLCEAIHEH
                     HQAEKTFADERADGRKKTLLAILKMAEHICASYKTVGKQDKDYEWARIGQTILIYVGL
                     TEYDFENITALIGEMGLGSSDFCNF"
     gene            10749..11930
                     /locus_tag="PKZ52_00185"
     CDS             10749..11930
                     /locus_tag="PKZ52_00185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNG58209.1"
                     /translation="MNDIFLNAQADRRIKRGHLWLYSNEIDVHRSPLKGLTAGQIVNV
                     FTHSGAPLGTAYVNPQALICGRLLSRQVDAKIDRAFFVKAIERALRLRSRCFAQPYYR
                     LVYGDADFLPGLVVDRYGDYCVVQMASAGIDQMAEPILTALEQVLKPKGIVVRNDHSA
                     RELEDLPEQCSTRGEVPEILRLEENGVPFEIPALTGQKTGWFYDHRINRQFLAPLCQG
                     KRVLDVFCYLGSWGIQAAVAGAAQVALVDASASALEGARRNAELNGVAHKVTCHKGKA
                     IEVLKNLLAEGEKFDVVVLDPPAFIKKRKDQKAGEAAYRHINELAIRLLEKEGLLVSA
                     SCSMPLENAVLTDLVRGAARANDRNAQLIWTGGQGPDHPIHPAIIETAYLKAQFFAIS
                     S"
     gene            complement(11937..13607)
                     /locus_tag="PKZ52_00190"
     CDS             complement(11937..13607)
                     /locus_tag="PKZ52_00190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG58210.1"
                     /translation="MDKEYQTAPDTSAGIQAPKLNAFRRLQACALLCALVWLLGAALT
                     TRYLAEQNLAADLANEEKSLIKETGHILNTLEQTMHQAEQLSKTLSFDRSIIDLAKTA
                     NQHTAQFNTLDQAARAQFTLNLPGAAAVNSLFAQLAQHIEMEQLFALNKTGYCIGSSR
                     ADQADGCIGVNYLTRDYFQAAKAQGSGRQFAIGRVYPTPSFFFSTAIKEAEDFAGVIV
                     VRQKVGHIVRFLAQQNLRVAVTDKNGIVLSSNREDMLFQQIAADGQALPSKSDIQRIF
                     QRDNLRILPLKFIASPRPELKLVALDGKRYLLFGSALAAGDFLVYVFNPIEPLFLAQQ
                     KYWATGGIIIVLGLLVILLIERNINYTQHRLAHLNALSAANQSLALASQELYFLSVTD
                     VLTGIANRRFFTRRLKEEIDRHGRAEPSQTQLETTLALLAIDIDFFKTINDTHGHPAG
                     DQAICALASICKNTIRPYDILGRIGGEEFAVLLIDITAPQAEEIAERIRSVCETSSIS
                     VEKAIFNQTCSIGMALYQPGESAEQLLAHADRALYAAKHNGRNCVRVF"
     gene            complement(13737..14030)
                     /locus_tag="PKZ52_00195"
     CDS             complement(13737..14030)
                     /locus_tag="PKZ52_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HNG58211.1"
                     /translation="MTFYRWQGEDLILQVLLQPKASANQWVGRVEGRIKIRLTAPPVE
                     GQANSQLIEFIAKQFKVAKSQVQLLSGEQARQKTLHIKAPRLIPAILAIEKDG"
     gene            complement(14035..14622)
                     /locus_tag="PKZ52_00200"
     CDS             complement(14035..14622)
                     /locus_tag="PKZ52_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNG58212.1"
                     /translation="MSTVISIIQFLLNTLGSLYLLAICLRFLMQLARADFYNPLSQLV
                     VKLTNPLLLPLRKIIPGLWGIDMASVVLALLFHTLMVELMILVQHGVLYMPFAVLYWA
                     LIGTVLFVSHIYLILFFVVMIASFIAPYSSNPALVLCYQIVEPVLAPFRKIIPPAGGF
                     DFSMLFAGLVIMVVRMALQGVAASSGVPLGMLLGF"
     gene            complement(14667..15491)
                     /gene="proC"
                     /locus_tag="PKZ52_00205"
     CDS             complement(14667..15491)
                     /gene="proC"
                     /locus_tag="PKZ52_00205"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNG58213.1"
                     /translation="MASISFIGAGNMARAIAQGLINTGTPSQQLLLTGPRPEHLSGIA
                     RELGVVTSTDNRAAVEQADTVVLCVKPQIMAAVCKEIARHLKPTSLVISVAAGIRLAS
                     LSQWLGGHRAIARAMPNTPAQIGLGATGVYASALSADQRQRGETLLAATGLVQWVEEE
                     RLIDSVTALSGSGPAYFFLFMEAMIAAAEAQGLSPASAQALCIQTALGAASLASQTGV
                     APAELRRRVTSPKGTTEQAIHQFETGGLKDLVKTAMDACQARAHSLADELGGAPSL"
     gene            complement(15512..16186)
                     /locus_tag="PKZ52_00210"
     CDS             complement(15512..16186)
                     /locus_tag="PKZ52_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNG58214.1"
                     /translation="MHKIAHNLTQVLNRIQAAAEGCGRKAQDITLLAVSKTQPAAAIS
                     EAHAAGQRAFGENYLQEALEKIAELQSLNIEWHFLGPIQSNKTRPIAEHFAWVHSLAR
                     ASIAQRLNAARPAALGPLHVCIQVNADGQASKAGVALDEVAALAEQVQRLPNLRLRGL
                     MAIPNPADPTPAFAALAALQAELNRRYSWQLDTLSMGMSDDLDLAIAAGSTLVRVGTA
                     IFGPRH"
     gene            16287..17321
                     /locus_tag="PKZ52_00215"
     CDS             16287..17321
                     /locus_tag="PKZ52_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNG58215.1"
                     /translation="MDITELLAFSAKQRASDLHLSAGLPPMIRVDGDVRRINLPPMDH
                     KQVHSLIYEIMNDKQRKDYEEYLETDFSFEVPGVARFRVNAFNQNRGAGAVFRTIPSK
                     VLSMEELGMGQVFRDISSVPRGLVLVTGPTGSGKSTTLAAMIDYINENKYHHILTIED
                     PIEFVHESKKCLINQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRVVDVFPAQEKSMVRSMLSESLEAVISQTLLKRIGGGR
                     VAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGANVGMQTMDQCLADLLERRIITRENA
                     REKAKMPDKF"
     gene            17334..18476
                     /locus_tag="PKZ52_00220"
     CDS             17334..18476
                     /locus_tag="PKZ52_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNG58216.1"
                     /translation="MDIDRLLQLMVEKGASDLFITAGVPPSMKLHGKIVPVTTTPLSP
                     EKSRETVLSTMNEKQRAEFVERKELNFAISVRGVGRFRASAFYQRNLAGMVLRRIETN
                     IPKIDDLGLPEIIKDLAMAKRGLIIFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
                     PIEFIHQHQGCVITQREVGIDTESFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFA
                     ETGHLCLCTLHANNANQALDRIIHFFPADRHSQLWMDLSLNLKAIVAQQLIPTPDGSG
                     RRACLEVMLNTPLASDLIRKGHVHELKELMKKSTELGMQTFDQALFDLYEGGSITYED
                     ALAHADSPNDLRLMIKLRSETDADYLKNAADELSIQEDESHRVRRF"
     gene            complement(18977..20293)
                     /locus_tag="PKZ52_00225"
     CDS             complement(18977..20293)
                     /locus_tag="PKZ52_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNG58217.1"
                     /translation="MSQPKPPVPKLPPSCTSQPITWVGIVCMLACVFSLRFLPTMALS
                     YQVVLILACYVVPIAILEWKYLKPQLRPSSGLDLINPPKPANSARVATKLFGLYATFG
                     LMALFYSLIPEYIGGFYTRYWRFIADLAIVFVPASVLYFAWVDRRMRHPEDGYYQLGR
                     FLLGKTNGLNTAKLRQHLTGWGVKFFFLPLMFIYALSFFEKAQGWNLFDWEFWQNKGA
                     TVPTDLFYGIDVVIVTVGYLCTLRIFDSHIRSAEPTAAGWLWALMCYEPIWSFVAASY
                     INYNSDNFEWYQWLDGNLVLQGIWGGAILILTFIYMLASVGFGLRFSNLTNRGIITNG
                     VYAWCKHPAYVSKNLSWWLIAVPFVLHDKATYVTVMRDCAALLALNGVYFLRARTEER
                     HLSSDPTYVAYALAMNERSIFAPLARKLPFLQYKPSQALVDLKEYQ"
     gene            20540..22060
                     /locus_tag="PKZ52_00230"
     CDS             20540..22060
                     /locus_tag="PKZ52_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNG58218.1"
                     /translation="MRQNLLKEVLLSTLCLALILLAGCGDTKVTTRSLDGQSSGPTWT
                     QNQYASFKDYLNLCAAARTGIDPMTGKPFLDRAGSASLEKFALRSFSHQTYLWASQLE
                     DKDPTTNLNPQAYFELLKIPADRFHFYVSSEENYKEFILGKALSYGITWFRDGNGEIV
                     VAYVERNSPAEIAGVVRGYRVVQMDGFAPAALSNEQLIAAAYPTEQSPVHRFVFKNSQ
                     GVEQPEVRLSAAEVQVNSVQNLQYFTAANERKVAYFSFGSFNGTGEKELINTVNEINQ
                     TGVQELILDLRFNGGGYLDLASELAYMVGGDGVKKTSVFSALRFAPGNTEYRDEVIPF
                     WPTSRALSSDVAKDQPLPTLNLKRLYVLTSASTCSASESLINGLRGADFPVVQVGTTT
                     CGKPYGMVPLSNCGFDYYTINFRYENAKGFSEFESGLAPRNSVQTQGSPLGCEVADQY
                     RDPLGSANESLLAAALKHIDSGSCGSAALAAPRAQGSSLPHPNPVFSNAIGPRLSF"
     gene            22358..23644
                     /locus_tag="PKZ52_00235"
     CDS             22358..23644
                     /locus_tag="PKZ52_00235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG58219.1"
                     /translation="MRIVLTRHDKWLLGAIAFSLLCLPLPHLLPMRVTSLLQAQVSLR
                     SSIYADSSDQEVQWLNKEKLHWICRVRAEYSAKACGFSLAFDPSLDWQDAYGLRIALA
                     AQSKNQFFRLYVRNHVPGAAGTLPEDAQYNQMMLPAQDANQPVDIAFSEFALADWWLA
                     SHPVANEYNIARFDHVSSLGIDLDSPAALGDHELQLAQLALLRPYISVQTCYLSLLGL
                     WTALLLLLSLQRLWLRRLLLAQARANIESQKPAVVEFEESAPAFCEIPQDDLTGIYNR
                     QGFIDYWSQASRLWADNDSMGLLVVNLDQYTSIGESFGRDAKDQALLALADALKSHMR
                     PQDKLARWHEGQFILLCPLTGHAQALQLAEKLRHHINLISFDEPAPLSLSASIGVILF
                     PAQALLEQAYNRCELALNTTASNGNRVIDCGVLNLD"
     gene            23637..>24387
                     /locus_tag="PKZ52_00240"
     CDS             23637..>24387
                     /locus_tag="PKZ52_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58220.1"
                     /translation="MTKRPYFIAAWLLTAMAVIALACFPLLPKRYWKLGEESQFNRLL
                     ITDKNKGGQTEAKWLGARRYQCVIQPQTQDPFCAMQISLKDEAGRGRDLSQFDRLRIK
                     VRLQGPGSVLRLHMRVFDGRFSKPGQMDTAKFQGYVFSPGEQGQVLELPLAGFSVADW
                     WVREFAPKREYAQLDFANVLMIGIDIPAPTQAGTYDIEVEELEFIGSRVQLASVLVVF
                     ILLWCALVAVYLLAGIWQVARQWQRELPAWRE"
BASE COUNT         5739 a         6490 c         6549 g         5609 t
ORIGIN      
        1 attacaccgg ctgatcgcgg cggcgccgca aattcactgg cttagcgata gcctgcagga
       61 ccttacgcgc gagggggagc tgtgcctgct gcacaccgcc gcaggggcga ctattcaaac
      121 gccgctcatt ctcgccgccg atggcgcccg ctctgccgtg cgcagcctgg cggccatccc
      181 ggtgcgccag tggtcctatg gccatacggc cttggtctgc cgggtacata ccgagcgccc
      241 ccatcgccat accgcctacc agcgcttcac ccaccagggc cccctggcgt ttttgccgtt
      301 attcccggcc gggcagcagg acagctctat tgtctggtcc ttagataaca cacttgccga
      361 gcagatgatg gcgctgagcg aggacgagtt taaaaccgcc ctggccgccg cccttgaaca
      421 tcgcctgggc gccgtgttgc agtgtgatgc ccgcctcgcc ctgcccttac atcaatgcca
      481 cgcccgccgc tactgcgcac cgggagtcac tctgctgggg gatgcggccc acgccattca
      541 cccactggcc gggcagggcg ttaacctggg cctggccgat gtggccgctt tagtggatga
      601 actggcccgc gcccaccaga gaggcctgcc ccttgcccac cccagcgtac aggatcgcta
      661 ccagcgcgcc cgtatgcccc acaatttggc ggccatggcg gccatggaat ccttcaagcg
      721 gctgttcggc aacagcaacc cctatgtggg cctggtgcgc aacctgggcc ttaagcttgc
      781 ggggcagctg ccgggggtga aacagcagtt tatgcgcttg gcgagcggcc gctaatggca
      841 ctcgggccag caaagaccct ggtcagccag cagacctggc agatctgccc ccattgcggc
      901 gagttgttgc gccgggtgcc ggcctgtgaa gagcaggcgc tcttgtgccc caactgccag
      961 ctgccgctac cggcggccaa aaccgcctgg gtgcaaccgg ccacggccct gtcggtggcg
     1021 gcggtggtgt tgtttattat cagcaacagt tttgattttt taagcctaaa actggtgagc
     1081 tttgagcaga gcaccagtat tctcgcgggc gtgggcgccc tgctagccca ccaacagtgg
     1141 ctgctggcgg ccctggtgtt ctcaacgata tttttattcc ccctgctgga agtcgccgcc
     1201 ctgttgtatc tgttagtgcc ctttcagctc aagcgccgcc tgcccggcca ggtggccgtg
     1261 ttgcgctggc tgatccgcgc ccagccctgg agcatgctgg agatttttct gctggcgatc
     1321 ctggtcacct cggtgaagct tggcgacatg gcaagcctta tccccggcgt gagcatgtac
     1381 gccttttttg ccctggtgcc gattctggcc atcgcctatg gggttatcga ccggcagagc
     1441 ctgtggagtt atctgcagcc ggataattgt tatgtagccg agccttccga tctgctctac
     1501 gcctgcggtg tatgtgaagc catggtgggg gagacgctgc tgaatcacag cgcccattgc
     1561 ccgcgctgcc taagccccat gcacaagcgc attcccaaca gcctgcaaaa aaccaccgcg
     1621 ttaattgtgg cctcggtgat tttatatatc cccgccaacc tcttaccctt tatgaccacc
     1681 cgcaccctca ccggggagcg cgacgacacc ctggtgagcg gggtcatcag cctgctcaac
     1741 cacgatatgt ggttaattgc cgccgtggta tttatcgcga gtatagtggt gcccatggcc
     1801 aaactggggg tgctgattta tttactggcc agcgcccact ggcagcacta ccgcaaccaa
     1861 gccaccaaaa ttcgcctgta tcacttaacc gaattaatcg gccgctggtc catggtggat
     1921 gtgtatgtgg tcaccctgct taccgccctg gtgcagtttg gttttttagg caatatccag
     1981 ccgggcaatg ccctggtggc ctttgccgcc gtggtggtat taactatgct cgccgctgag
     2041 actttcgacc cgcgtttatt agacaccccc caggaacagc ctggggattt tccggcccat
     2101 gcgtttaaac atgaatctac ctaaaccgat ggttacaccc ttccaaaaac tctcgccggt
     2161 gtggttaatc cccctggtag cgctgatcat tgccctgtgg ctggcgatta attcctggca
     2221 ggaaaaaggc caggaaattg aaatcctttt aaacgcggcg gcgggcatcg aagtgggtaa
     2281 aacccaggtg cgcctaaaag atgtgccggt gggcaaggtc tccaaaatgc agctcaccgc
     2341 cgatttaagc aaagtgcgtt taaaagtcac cctagagcgg gaaattgcca aacacctaag
     2401 tgtgaatacg cgtttttggc tggtgagccc gcgtattagt gtggcggggg tttccaacct
     2461 cggcacccta gtatctggtg tatatatcgt gatggaccct ggtgaacccg gcgccttttc
     2521 caccaatttt gacggcttac cggagccacc cgccatagaa tccagcgtca aaggcaccac
     2581 ctttttattg cgcgccgaca gcctagattc ggtggatata ggctcgccgg tgtattaccg
     2641 gcaattaaaa gtcggcgaag tcaccagcta taaactgagc gaaactggcg aagcggtgga
     2701 aattcgcgtg tttgtgcagg ccccccacga taaattaatc accagccaca gccgtttttg
     2761 gaatgtcagc gggtttgata tcaacctggg gccagacggt gtaaaggcgc aaatggcctc
     2821 gttagtgtcg tttttaggcg gcggtatttc ctttgaaaat atcaacgcct atgaaaacgc
     2881 ggaacctgcc aaagccaatc aaaccttttt tctctacgcc gataaagaat cggtactgga
     2941 aggccgcttt agcgccaaat tttattatct gctgaaattc tcccactccg tgcgcggctt
     3001 aaaagccggt gcgccggtgg aatttcgcgg cattaaagtg ggcgaagtgg tggatgtggt
     3061 gttatccagc attgaaaacc aagataaatc cctgctggtg tatatcacct tagagcctga
     3121 gcgttttaaa gcggatttag aacccaagcg ggaaattatc gacggcatgg tggccaaaat
     3181 ggttgaccag ggcctgcgcg gccaattgcg caccgacagc ctattaaccg gcagcctgta
     3241 tgtggattta gcctttgaaa acaaacaaaa acccggcaaa ttaaccgtca ccgacaacca
     3301 tgcggaaatt cccaccctcg atgacccgct ggatcagctc accaaacagt taagtgaaat
     3361 taccgataaa tttaaccgta ttcccagcga taaaattggc gccgacctgg ctaaatccat
     3421 ggcgagcttg gccaatataa tgaataccct ggataagaat aataccgctg gcaaactcga
     3481 tggcaccctg ggcaatcttg aaaaagccac ccacgacatg gacaaaacag tagccgaggc
     3541 cacccaaacc ctcagccaat tgcgggtgac tttaaaaagc ctggatgcca cttttgcgcc
     3601 ggattcccaa ttgcattttg aagtaattga aatgctcaat tccgttaccg aagcgtccga
     3661 ttcgtttgat cgcattgccg atgaattaaa acgctatccc aacgccttaa tattcggagg
     3721 caagaaaaaa gaatgatcgc ccgcggcctg ttattcagtc tgttgctgtg ggggctatgt
     3781 gcctgcagtc agcagaccca ttattacacc ctgcaccaca ccaagggccc caaagcgccc
     3841 ctgggcagct acacatttag cttgggcata ggcccggtat tcctaccgga aagccacaac
     3901 caaccggggg tggtgagcct gaccgaaggc caggaagtca ctgtggccca gtaccatatt
     3961 tgggccggtt atttaaatca gtcggtgacg cgggtgctgg ccgataattt atccctgctg
     4021 ctcggcgccg ataccgtctg gccctttccc tgggataacc gcatgcgccc tgtgcgccag
     4081 gtgcgggtaa ttattgaaga aatggccggg cagcgaggca aaacagtggc cctacaggct
     4141 aaatatattc tcaccgccga caacggcgag cgcaccctgg ccgccgagcg cacccgcatt
     4201 gaagtgccct gcgctggcga ttcctacgaa gcctatgtgg cggctattaa ccaggcgctc
     4261 aatcaattat ccgaacagat ggccaaaacc ctgatcgcca aacaataggc ttttagccgc
     4321 cgtaacttac aggtatctac cccactattc ctatgttatg tgaaatagat gcatgaagat
     4381 tctatatgtt gaaaaccatc cgtacttcgg gaaaattgtg aaagacaaat tccttagtga
     4441 atttcatgta actatagtgc caagcattaa gtccgccact gaagcagtaa atcaatcgag
     4501 ttttgatata gcgttagtgg attacgactt ggatgatggc aaaggggatg aattcatcgc
     4561 ctacttaata agagcaggta ttgatttacc ttgtatagca gtttcatccc atgaaaatgg
     4621 gaattcagct ctagttaaag ctggagcagt aagtgtttgc agtaaaatgc atttctcaaa
     4681 aattgcttct gtggtgggta aaattaatgt gtaaagtaaa acaatcacat aacaagcgtg
     4741 tccatttgac actttggcct acgttgcgct ttggggtggc tgcgccactt taccccaaag
     4801 ctccactacg gccaaagtgc aaatgacacg ggcgttaaat agaattttaa ttatccctct
     4861 gctgcttagc gcctatggct gcgactccag atcccagccc cagagagagc tgactaggcc
     4921 cgcagcggag gtgaaagcgg tcattcccag cgccatagac gcagccgcgc cgggggcggc
     4981 gcaagcggtg agtgccgagg atattaaaca ggcccaggag gccgccgccg atagccccca
     5041 gcacgaataa aaatctgcct ctgggtttgc cagcggcttt taggataaga tgcttttttg
     5101 tatcaatcgt taattaaatc aacctgttga ggagcgcaaa tatgcggtta aaaaaatgtg
     5161 tgatggtgtt gggtctggcg gcatttttac agtccacaac cggttgcggt ttactcttac
     5221 accctgagcg ccagggctcc aagggcggca acctggaccc tgtcatagtg ctgctggacg
     5281 gtttgggttt atttttcttt ttagtgcccg gaatagtcgc ctttgcggtg gatgtctcca
     5341 ccggctgtat ttacaccggc ggcggtggtg gcggtggcaa gcgccgtcat ttctcctcca
     5401 tcgagcaagg ccagccaatc aacgccgatg aattggcggg catgaaaaaa atcgctttaa
     5461 acggcccggt gactaaagcc tccatcgaag cggccctaga gcgggaactg gggcaaaaag
     5521 tagatatcac cgccgccaat gtacaggtgc aacctatttt ggaacccgcc aaaatttaat
     5581 acttaacccg gctttggccg ggttttttta ttgcgaccct tgttattaaa aagtacggtt
     5641 atgaaaaaat atgtttttct ttttgtcttg gcctgtaccg ggctgttaag cgcctgtaat
     5701 ccgctggcta aatttcagca accgcaggta cagttaagca atttggtggt gctggagcgg
     5761 cacggcttcg agcagttatt tttgctcaaa ctcaaagtca ccaaccccaa taaaatggcc
     5821 ctacccattg cgggtttgca ataccaggtg cgcttaaacg gttttgatgt atttaccggt
     5881 aacgctggag atttaccgca attaaaagcc tatagtgata cctacattga attgccggtg
     5941 agcgccaatt taattagtgc cggggccgtg ttggccagtt tattagtcag tgaacacccc
     6001 agcattaatt atcaattaaa cgctaccttc gatttaaccg gtgtgctgcc ggattttcgc
     6061 gtgagtgaaa aaggcctggt caatttagaa cactaataac cgcagaggtg aaattcacca
     6121 caacctgaca gcctgttgca atacaagcgt gccagagcgt cgtcgcccaa gtatttcaac
     6181 aagctgctag gtatctcaac aggctgctaa agagccgtct tagctaaatt gcaggaggaa
     6241 tccaccatga ataccctgtt tgattttccc tttcgtttgt tcgatcaaag catcagcccg
     6301 gttaccagtt ggttctcgcc caattatgaa attaattttg cgggtgataa aaacctggaa
     6361 caaaaaataa ttgccgacgt agccagctac ggcacccagt taaaccgcat taccgaggcc
     6421 ttgctggtgt tggcagaggg ccgtgacgaa cctgcgatcc aggagctgcg caaggtgcat
     6481 gagaacattg aacaggtgaa gacccagact aaggctcagc tggtcagccg catcgagcgg
     6541 gatcttgcca agctcaaagc ccaggatgcc aaagcttatc agcagctttt ggctacactt
     6601 aaaccaggcg acgcagaata agcactcgcc ccaaccttaa gcgctaaaag cggccccgcc
     6661 ctcggttgtt ccggcaggta ctgcccgctg ttcagaggcg tacaaggtat gtgctgccac
     6721 aaaagtgtca taaaactcag gcagcctacg gcttatgatg gcctgaattt caggcgcgct
     6781 ttatagaggt aaaccatgtc cgaaatgccc gatttatccc aggaaccggc cggtctagag
     6841 tccgaagggc acaattttga tgcgccgcgt tattatttaa accgcgaatt aagccatctg
     6901 tattttaatc agcgggtgct ggcccaagcg ttggatgaga gccacccgtt gttagagcgc
     6961 ctcaattttt tattaatatt tagctccaat ctggatgagt tttttgaaat tcgcgttgca
     7021 ggcctaattc aaagtattaa atatcagcgg gaaatgtccg gccttgacgg catgcaggcc
     7081 aaagaaagcc taaaactcat cagcgaaatt tgccaccgca tcgtcgatca ccagtatcaa
     7141 attctcaacg atgtattagt gccggaactg caaaaacagg gcattcggtt tatccgccgc
     7201 aaccagtgga gcgccgaaca cagcgcctgg gtggaagaat attttcaaaa agaattagtg
     7261 ccggtgctaa gccccatcgg cttggattca gcccacccct tcccccgttt ggtgaataaa
     7321 agccttaatt ttattgtttc ccttgcgggc aaagacgcct tcgggcggga tttgggcctg
     7381 gccattgtgc ctgcaccgcg ctccctgccg cgttttattc ccctgccgcc acacttaaat
     7441 agcggcaatg attttgtttt tttatcctct attattcacg cccacaccgg caaattattc
     7501 cccggcatga cggtaaaagg ctgttatcaa tttcgtatca cccgcaatgc ggatttggat
     7561 gtggatattg aaggcgccgc cgatttagcc cgcgccctgc gcggtgagtt acattcccgc
     7621 cgatttggta cggccgtgcg catcgaagtg gccgataact gcccgaaaga aattaccgat
     7681 tatttattgc gggaaaccgg ccttacggat gacgatttat atcgcgtcaa cggcccggta
     7741 aatttaaaac gcctcatgcg tttgcctatg atggtgaatc gcccagatct gcgttttcca
     7801 ccgcttattc ccagcatccc caaagggttt caccgcaagg acaatatttt tgaggctatc
     7861 agcaaaaaag attatttatt gctgcatcct tttgaatcct tcgcaccggt ggtgcaattt
     7921 ttacggcaag cggctaaaga cccacaggtg gtggcgatca aacaaactct gtaccgcacc
     7981 ggcaacgatt cggccatagt ggacgcccta gtggatgccg cccgcagcgg cacggaagtc
     8041 accgtggtgg tggaactgcg ggcgcgtttt gatgaagagg aaaatttaga actagccagc
     8101 cgcctgcaag aggcgggcgc cgtggtagtg tatggtgtgg tgggttataa aacccacgcc
     8161 aaaatgattt tggtggtgcg cagggagcac aatcagtaca agcgctactg ccatttaggc
     8221 acaggtaatt accacgcggt taatgcccat ttatatacgg attattcgct gttaaccgcc
     8281 gatgatatga tcggtgaaga tgtgcaccgc atttttcacc agctcaccgg catgggcaaa
     8341 accgagcgca tgcaaaagtt atttcacgcg cccttcaccc tgaagcggca gttggttaaa
     8401 ttcatcgaca acgagataaa agccgcccaa gagggcaagc ctgcgcgtat tatttttaaa
     8461 tgcaacggtc ttactgaacc gaaaattatt cacagcctgt acaaagccag ccaagcgggc
     8521 gtaaaaatag acctgattgt gcgcggtatg tgttgtttaa aatccggcat tgaaggcatc
     8581 tcggataata tccacgtgta ttccattgtc ggccgctttt tagaacacgc ccgcgtgtat
     8641 tattttgcca acagcggcgt atattgtgcc agcgcggact taatggagcg caatttaaat
     8701 cgccgggtgg aggtgtgttt ccccatcgaa gatcaagccc tgagcgcccg agtgttagct
     8761 gagcttgcgc tgtatttaaa cgacggcaaa cagcgttggg tgcaattgga gtccggccag
     8821 tatcagcggg tggaacagga cggccctgcc gcccaaatgg aattaatgcg actgctaacc
     8881 agcccttcaa cctaaaaacc gcagttgaac tctactacgg tttttaggtt caaattaaca
     8941 ctcaactagg gcgcgtaaag cgcccttttt tgtgccgctt gtttgtgaca cctcaaacga
     9001 caaccccaag ggtgaaaaca aatagcagag tatttgtgca aagccggggt atttaaccta
     9061 aaaaccgcag ggcttaatcc cgcaacatct tatgtacgcc gaacaatttc ggtttcccca
     9121 tccagggcaa agcgtgcacg gatcgcactt tgcgaataat acccctctga acatgtaaac
     9181 ctcccttaga tttacgccat attcagaatt agcggcctag gatcgtcact ggggccacag
     9241 ccaaccctga gcattggttt tgttcagtag atatttaatc gattgcttct tgtgcatgct
     9301 taggcgccaa ccaccataaa tcacaggatt tgcttatgaa acgagtatta atgacgggtg
     9361 taattttatt ggccgccaac agctatgccg acaccctgcg ctgcggcgct aaattaattt
     9421 ctactggcga ctcaaaaggc agcgttgaga atatttgcgg cgcacctgcc ggtacagatt
     9481 cctactgtaa gcccactaaa tcaaccaact ctacaagttc taaagagcaa gactgcgtgc
     9541 gcgtggatat ttggcgctat aaccggggcg ccgggcagtt gggtgcagag gtggaatttc
     9601 aaatgggtga ggttactgcc attcgcaata ccgagcgaga ataatacaat tgcgccacag
     9661 gcttgtacgg aggtgaggat ttaatcttgg gacttggcgg agcttgccca gctgcttatc
     9721 gataatccag cagcgaaaag gcagcgcctt aaaaattgca aaagtccgaa ctgccaagcc
     9781 ccatctcacc aatcagggcg gtaatatttt caaagtcgta ttcggtcagg cccacgtaaa
     9841 ttaaaatggt ctggccgatg cgcgcccact cataatcttt atcttgtttg ccgacggttt
     9901 tgtagctggc acaaatgtgt tcagccattt ttaaaatcgc cagcagggtt tttttgcgac
     9961 cgtcggcccg ttcatcggca aaggtttttt ccgcctggtg gtgttcgtgg atggcctcac
    10021 ataaatagcg cggtaaattc cagctttttc ccacgtaata acccaccacc gcatggttgg
    10081 tgcgaattaa atggttttct gtggtggtaa tggcctgctc tgggttggcg taggcttcaa
    10141 tttgcacctc taaataattt ttaaagcgca ccattaacag cggtataccg cagttgtgaa
    10201 ataaccctag ggtgtaggct tcatccgggt agggaaaacc tatgtgtttg gcgatggcgg
    10261 cggaggtcat ggctacatcc atggcgctgt cccaaaagcg gccgagggca ataatatcgt
    10321 cgtcggacag ggcgccgcga atggactggg cattcaccag gttaaccacc gaattaaccc
    10381 ccaataaatt caccgcctga tgaatggagg taattttatt gctgtgttta aaaaaaggtg
    10441 aattaacggt ttttaaaata ctgccggata aacccacatc ctggctgatg aggtgtgcga
    10501 tggtgtcgat gctgcaattg ggggagcact gctccatttg taaatccacc attacctgcg
    10561 gctgtggcgg aatattgatg ccctgcagta tccgcgaaat ctgctccggg gttaactcag
    10621 cgttcatggc gaaggggttc acgaaggggg tcaaaaggta cctagtttga gtgtagcagc
    10681 cttctccgta tttgccccct ccctgggtga tgcggctata ctcgccggtt tcttgttaag
    10741 gcgctcccat gaacgatatt tttttaaatg cccaggccga ccgccgcatt aaacggggcc
    10801 atttgtggct ctattccaac gaaatcgacg tgcaccgctc cccccttaaa ggcttaaccg
    10861 ccggtcaaat cgtcaatgta tttacccatt cgggcgcccc cttaggcacc gcttatgtca
    10921 acccgcaagc gctgatttgc ggccgcctgt taagccgcca agtggatgcc aaaatcgatc
    10981 gcgccttttt tgtcaaagcc atcgagcggg cgctgcgcct gcgcagccgc tgttttgccc
    11041 aaccctatta ccgcttggtg tacggcgatg cggatttttt acccggcctg gtagtggatc
    11101 gctacggcga ttattgcgtg gtgcaaatgg cttcggctgg catagaccag atggccgagc
    11161 cgattctgac cgccctagag caggtgttaa aacccaaggg catagtggtg cgcaatgatc
    11221 attcggcgcg ggaattggag gacttgcctg agcagtgctc cacccgtggc gaggtgccgg
    11281 agattttgcg tttagaagaa aatggcgtgc cctttgaaat tcccgccctc actgggcaaa
    11341 aaaccggttg gttttacgac caccgcatca accggcaatt tttggcgcct ctctgccagg
    11401 gcaaacgggt gttggatgta ttttgttatt taggcagttg gggcattcaa gcggcagtgg
    11461 cgggcgcggc ccaggtggcc ctggtggacg cctcggccag tgcactggaa ggcgcccgcc
    11521 gcaacgccga gctcaatggc gtggcccata aagttacctg ccacaagggc aaagccatcg
    11581 aggtgttaaa aaacctgctg gcggaagggg aaaaatttga tgtggtggtg ttagatccgc
    11641 cagcatttat taaaaaacgc aaagatcaaa aagccggtga agcggcctac cggcatatta
    11701 atgaactcgc cattcgcctg ttggaaaaag aaggcctatt agtatctgcc tcctgctcca
    11761 tgccgctgga aaatgctgtg ttaaccgatt tagtgcgcgg tgccgccagg gccaatgatc
    11821 gcaacgcaca attaatttgg acgggaggac aggggccgga tcatcctatt cacccggcga
    11881 ttattgaaac ggcctattta aaggcgcagt tttttgccat ttcgagttaa gggcacctaa
    11941 aacacccgca cgcaattgcg cccattatgt ttggcggcat ataaagcgcg atcggcgtga
    12001 gctaataact gctccgcgct ttcacctggt tgataaaggg ccatgccgat ggagcaggtt
    12061 tggttaaaaa tggccttttc cacggaaata ctgctggttt cacacacgct gcgaatgcgc
    12121 tcggcaattt cttcggcctg tggcgcggta atgtctatta ataacacggc aaattcttca
    12181 ccgccaatgc ggcctaaaat atcgtagggc cggatagtat ttttgcaaat actggcgagg
    12241 gcgcaaatgg cctggtcccc agcggggtgg ccgtgggtgt cgttgatggt tttaaaaaaa
    12301 tctatatcaa tggccaatag ggccagggtg gtctctagtt gagtttgcga aggttccgcg
    12361 cgcccgtggc ggtcaatttc ttcttttaaa cgccgagtga aaaaacgccg attggcaatg
    12421 ccggttaaca catcggtgac tgagagaaaa tacaattctt gggaggccag ggccagtgat
    12481 tgattggccg ccgagagggc atttaaatgg gccaggcggt gttgggtgta attaatattg
    12541 cgctcaatta atagaatcac caataaaccc aacacaataa taatgccgcc agtggcccaa
    12601 tatttttgtt gggcgagaaa aagcggttcg atggggttga agacataaac taaaaaatct
    12661 cccgctgcca gcgctgagcc gaataataaa tagcgtttgc cgtcgagcgc caccagtttt
    12721 aactccggcc gaggagaggc gataaatttt agcggcaaga tgcgcaaatt atcgcgctgg
    12781 aaaatccgct gtatatcgct tttactcggc agcgcctggc cgtcagcggc aatttgttga
    12841 aataacatgt cctcgcgatt gctgctgagt actatgccgt ttttatcggt gacggcgacg
    12901 cgcaaattct gttgggcgag aaagcgtaca atgtgcccca ccttttggcg caccacaatt
    12961 acgcccgcaa aatcctccgc ctctttaatg gcggtggaaa agaaaaatga cggcgtagga
    13021 tacacgcggc cgatggcgaa ttggcggccg ctgccctggg ctttggcggc ttgaaaataa
    13081 tcgcgggtaa gataattaac gccaatacag ccgtctgctt gatcagcgcg actggagcca
    13141 atgcagtagc cggttttatt cagggcaaat aattgctcca tttcaatgtg ttgtgccaac
    13201 tgggcaaata aactgtttac cgctgcggcg ccgggtaaat tcagggtaaa ttgcgcccgc
    13261 gccgcttgat cgagcgtgtt aaattgggct gtgtgttggt tggcggtttt tgcaaggtca
    13321 ataatcgagc gatcaaagga gagggttttg gatagttgtt cagcctggtg catggtttgc
    13381 tccagggtat ttaagatgtg gccggtctct ttaattaagg atttttcttc attggctaga
    13441 tctgcggcga ggttttgctc ggccaagtag cgggtggtta aggcggcgcc cagcagccaa
    13501 accagggcgc acaacagggc gcaggcttgt aggcggcgaa aggcattgag tttcggcgct
    13561 tgaatgcctg cgctggtgtc cggggccgtt tgatactcct tgtccattgc ataatcctct
    13621 tgggctcgca gggaggtagt tttggcacat gctgtagctg gccgcaatag gtgctttgtg
    13681 ttcccgtcgc caactttatc gccaccagag ctggctaggc agtattcaac aagccgctag
    13741 ccgtcttttt cgatggccaa tatagccggt attaaacgcg gcgccttaat gtgcagagtt
    13801 ttttggcggg cctgctcacc gcttaacagc tgtacctggc ttttggcgac tttaaattgt
    13861 ttggcgataa attcaattaa ttggctgtta gcctgcccct ccaccggtgg cgcggtgagg
    13921 cgaattttta tgcgcccctc cacccgcccc acccattgat tagcggaggc cttcggttgc
    13981 agcagcacct gtaaaattaa atcctccccc tgccagcggt aaaacgtcac ggcattaaaa
    14041 ccctaacaac atacccaagg gcacaccgga gctggccgcg actccctgca gggccatgcg
    14101 caccaccata atgaccaggc ctgcaaataa catggaaaaa tcaaagccgc cggctggggg
    14161 aattattttg cgaaaaggtg ccaataccgg ctcgacaatt tgataacaca acaccagggc
    14221 agggttgctg ctgtagggcg ctataaaact ggcaatcatc accacaaaaa acaaaattaa
    14281 ataaatgtgg ctgacaaata aaaccgtgcc gatcagcgcc caataaagca cggcaaaggg
    14341 catatacaac acgccgtgtt gcaccaggat catcagctcc accatcaggg tgtggaataa
    14401 cagcgccagc accaccgagg ccatatcaat gccccacagg ccgggaataa ttttgcgcag
    14461 gggcagcagc agggggttgg tgagtttaac caccaactgg gagagcggat tgtaaaaatc
    14521 cgcccgcgcc aattgcatta aaaaccgcag gcagatggct aataaataca aactgccgag
    14581 ggtgttgagt aaaaactgga taatcgaaat aactgtactc atggggatct cttattttcc
    14641 atcacgttta tggggggtgg gcggcgttac aggctaggcg cgccacccaa ttcatcggcc
    14701 aggctgtggg cgcgggcttg gcaggcgtcc atggcggttt ttaccagatc ttttaggccg
    14761 ccggtttcaa attggtgaat ggcctgttcg gtggtgccct tcggggaggt cacccggcgg
    14821 cgcagttcag cgggcgccac gccggtctga gaggcgaggc ttgccgcccc cagggccgtt
    14881 tgaatacaca gggcctgggc gctcgcaggg cttaggcctt gagcctcggc cgctgcaatc
    14941 atggcctcca tgaataagaa aaaataggcc gggccagagc ctgatagcgc cgtgacgctg
    15001 tcgatcaggc gctcctcctc cacccactgc acaagccccg tggcggccag cagcgtttcc
    15061 cccctctggc gctggtcagc gcttagggcg ctggcgtaca cgccggtagc ccccaggcca
    15121 atttgcgccg gagtgttggg catggcgcgg gcgatggccc tatggccgcc cagccattgg
    15181 ctgaggctgg ccagacgaat cccggcagca accgaaatca ccaggctggt gggtttaagg
    15241 tggcgagcga tctccttaca aaccgccgcc atgatctgcg gttttacaca cagcacgact
    15301 gtatcggcct gttccaccgc cgcccggtta tcggtgctgg taactacgcc taactcccta
    15361 gcaataccgc ttaagtgctc aggcctaggg ccggtcagca gcagctgttg cgatggggtg
    15421 ccggtattaa ttaagccttg ggcgatggcg cgggccatat tgcctgcgcc gataaaacta
    15481 atcgaagcca cagagggttc ctcataaggg gttaatggcg ggggccgaag atggcggtgc
    15541 ccacacgcac gagagtggag cctgcggcga tggccagatc aaggtcatcg ctcatgccca
    15601 tgctcagggt atccagctgc cagctgtagc gccggttgag ttcggcttgc agggcggcga
    15661 gggcggcaaa ggcgggggtg gggtcggctg ggttgggaat ggccatcagc ccgcgcaggc
    15721 gaagatttgg caggcgctgc acctgctcgg caagggcggc cacttcatcc agtgcaacgc
    15781 ccgctttact cgcctgtcca tcggcgttca cttggataca cacatgcaat ggcccgaggg
    15841 cggcaggccg ggcggcgttg aggcgctggg cgatgcttgc gcgcgctagg ctgtgcaccc
    15901 aggcgaagtg ttcggcgatg gggcgggttt tattgctttg aatggggcct aaaaaatgcc
    15961 attcgatatt aagcgattgc agctcggcga ttttttccaa ggcttcctgc aggtaattct
    16021 cgccgaaggc gcgctggcct gcggcatggg cctcactaat tgccgcggcc ggttgcgtct
    16081 tgctcacggc taaaagtgtg atgtcctgcg cctttcgccc gcagccctcg gcggcggctt
    16141 gtatgcggtt taatacttgg gttaggttgt gcgcgatctt gtgcatatac ttatcgaatt
    16201 gatgcaagct ttgttaattg gcttgccgtt acaatcagcc gccggcaccc atttttgtag
    16261 tttaggtttc cagttaaggt ttctttatgg atattaccga gttactcgct ttttccgcca
    16321 aacaacgcgc atcggacctc cacctttcag cgggcctgcc gccgatgatc cgtgtggatg
    16381 gcgatgtgcg acgcattaac ctgccgccca tggaccacaa acaggtgcac agtctgatct
    16441 acgagatcat gaatgacaag caacgtaagg actacgagga atacctggaa accgacttct
    16501 cctttgaagt gccaggagtt gcgcgcttta gggtaaacgc ctttaaccaa aaccgcggtg
    16561 ccggtgcggt atttcgtacc attccctcca aagtgctctc tatggaagag ctgggaatgg
    16621 gccaggtgtt tcgcgatatc agctcggtgc ccaggggctt ggtgctggta accgggccta
    16681 ccggttcggg taaatccact acccttgcgg ccatgatcga ctacatcaac gaaaacaaat
    16741 accaccatat tctcaccatc gaagatccca tcgaattcgt ccacgaaagt aaaaaatgcc
    16801 tgattaacca gcgcgaagtg caccgcgata cccacggctt tgccgaggcc ctgcgctcag
    16861 ccctgcggga agacccggat attatcctgg tgggtgagat gcgggactta gaaaccatcc
    16921 gcctagccct caccgccgct gaaaccggcc acttggtgtt cggcacattg cacaccacct
    16981 ccgccgccaa aaccatcgac cgggtagtgg atgtattccc cgcccaggaa aaatccatgg
    17041 tgcgctccat gctgtcggaa tctttagagg cagtaatttc ccaaaccctg ctcaaacgca
    17101 tcggcggcgg ccgggtagcg gcccacgaaa ttatgcgcgg caccccggcc attcgaaacc
    17161 tcattcgtga agataaggtg gcgcagatgt actcagccat tcaaaccggt gccaatgtgg
    17221 gcatgcagac catggatcag tgtttggcgg atttattgga gcgccgcatc atcacccgcg
    17281 aaaatgcgcg ggaaaaagcc aaaatgcccg acaaatttta attggagtag gccgtggata
    17341 ttgatcgttt attgcaactg atggtggaaa aaggcgcctc cgacttattt attaccgccg
    17401 gtgtgccgcc ctctatgaaa ttacacggca aaattgtgcc ggtcaccaca acgcccctat
    17461 cgccggaaaa atcccgcgaa accgtgctct ccaccatgaa cgaaaaacag cgggcggaat
    17521 ttgtcgagcg caaagaatta aactttgcca tcagcgtgcg cggcgtcggc cgctttcgcg
    17581 ccagtgcgtt ttaccagcgc aacctagcgg gcatggtgct gcgccgcatt gaaaccaata
    17641 ttcctaaaat tgacgattta ggcctgccgg aaattattaa agatttggcc atggccaagc
    17701 gcggcttaat tatttttgtg ggcgccaccg gtaccggtaa atccacctcc ctcgcctcca
    17761 tgattggcca tcgcaaccgc aattccaaag gccatattat ttccatcgaa gaccctattg
    17821 aatttattca ccagcaccaa ggctgcgtga ttacccagcg ggaagtgggc atagatacag
    17881 agagttttga ggtggcctta aaaaacactc tgcggcaagc gccggatgtg attttaattg
    17941 gtgaggtgcg ctcgcgggaa accatggacc acgccatcgc ctttgccgaa accggccatt
    18001 tgtgcctgtg taccctgcac gccaataacg ccaaccaggc gctcgatcgc atcatccact
    18061 ttttccccgc cgatcggcac tcccaattgt ggatggattt gtccttaaat ttaaaagcca
    18121 tagtcgctca acagttaatt cccacccccg atggcagtgg ccgccgcgcc tgtttagaag
    18181 ttatgttaaa cacgccctta gcctcggatt taatccgcaa aggccatgtg catgaattaa
    18241 aagaactgat gaaaaaatcc accgaactgg gtatgcagac ctttgaccag gcgctgtttg
    18301 atttatacga gggcggcagc atcacctacg aagacgccct ggcccacgcc gactctccca
    18361 acgacctgcg cttaatgatt aaacttagat cggaaactga tgcggattat ttaaaaaatg
    18421 ccgccgatga gttgtcgatt caggaagatg aaagccatag ggtgcgcagg ttttaagcga
    18481 atttcaggca ataaaaaagc gcggttttta ccgcgctttt ttattgccta agggttaagc
    18541 aaacgtaact attcagcatc tttttgtgtg gctggaggta attcctgcac tgactgggaa
    18601 ctaacgcgtg tgtgttttag ggctgttacc ccgtaataga gcgcccgcag tggtgactac
    18661 accgcgaatc aattatgccg gatggctaaa ccctcctgaa tttgccccac gcgcggaagt
    18721 ggggagtaaa gtgtgctgaa tagttacaag caaacaatac ttctacccaa gataaaatct
    18781 tgccaaatcc ctttaaataa acactccaac gcctccctag aaccacatct ttgcagtacg
    18841 ccgcaggcgg cgaaacgcca gataaaaatt aaatgtgctg atctcttacc aaacataaat
    18901 agatcctgcg actgcgcgca ggatgacggt tgggacgcag gcggcactgc ctggtagagc
    18961 atcctgagcc gaggttctat tggtattcct ttaaatccac caatgcctga cttggtttgt
    19021 actgcaaaaa cggtaatttg cgggccaggg gcgcaaaaat actgcgctcg ttcatggcca
    19081 gggcgtaggc cacataggtg gggtcggaag agaggtggcg ctcttcggtg cgggctctta
    19141 aaaaatacac gccgttgagg gctaagagtg cggcgcagtc gcgcattacg gttacatagg
    19201 tggctttatc gtgtagcaca aaaggcacgg caattaacca ccagcttaga tttttactga
    19261 cataggccgg gtgtttacac caggcgtata caccgttggt aataatgccg cggttagtca
    19321 ggttggaaaa gcgcaggcca aaacccacgc tcgccagcat ataaataaac gtcaagatta
    19381 aaatggcgcc gccccaaata ccctgtaata ccaaattgcc atccagccac tggtaccact
    19441 caaaattatc cgagttgtaa ttaatataag aggcggccac aaacgaccaa atgggttcat
    19501 aacacattag cgcccacaac catccagcgg cggtgggttc ggcggagcgg atgtgggagt
    19561 caaaaatgcg cagggtgcac aaataaccca cggttacgat caccacatca atgccgtaga
    19621 acaaatcggt gggcacagtg gcgcctttgt tttgccaaaa ttcccagtcg aataaattcc
    19681 agccttgcgc cttttcaaaa aaactcaggg cgtaaataaa catcaagggt aaaaagaaaa
    19741 atttaacccc ccagccggtg aggtgttggc gcaatttagc ggtgtttaag ccgttagttt
    19801 tacccaataa aaaacggcct aattggtagt agccgtcttc agggtggcgc atgcggcggt
    19861 ctacccaggc aaaatacagc acggaggcgg gcacaaatac aattgccaga tcggcaataa
    19921 accgccagta gcgggtgtaa aagccgccga tgtattcagg aattaaactg taaaacagcg
    19981 ccattaggcc gaaggttgcg tacaaaccga ataatttggt ggccacccgt gcgctgttgg
    20041 ccggtttggg gggattgata aggtctaaac cggaggaggg gcgcagctgg ggttttaggt
    20101 atttccactc caaaattgcg atgggtacaa catagcaggc gaggattaaa accacctgat
    20161 aactcagagc catagtgggc aaaaagcgca agctaaaaac acaggctaac atacacacaa
    20221 tgcccaccca ggtaatcggt tgtgaggtgc aggagggcgg aagcttggga actgggggtt
    20281 tcggctggga cataagcaaa ctctggcggc aggataaacg gcgggcagga taaacgcgca
    20341 agtgtagcga ctttggctat aaaaaccttg acctgtggcc cagcttggcg gtttgttcat
    20401 tcattcaggg cgggccttgg cgtacacttt caactcgggt aagcacctgg tttgcaggcc
    20461 tgttgctgat ttgcagcggt attaatggcc gggtgttggg tgttaataat ttcacatgtg
    20521 ttaatggagc ggtgtgccga tgcgtcaaaa cttattgaag gaagtgttgc tatcaacgct
    20581 gtgtttagcc ttaatcctgc tggcaggctg cggtgatact aaggtgacca caagaagcct
    20641 cgacggccaa tcctctgggc ctacctggac tcaaaaccaa tatgcatcgt ttaaggatta
    20701 tttaaacctt tgtgcggcgg cgcgcacggg tattgaccct atgaccggca aaccctttct
    20761 agaccgcgca ggcagtgcca gtttagaaaa attcgccctg cgcagtttca gccaccaaac
    20821 ctatttatgg gcctcccaat tggaggataa agaccccacc accaatttaa atccccaggc
    20881 gtattttgaa ttattaaaaa tacctgccga ccgttttcat ttttatgtgt cgtcggaaga
    20941 aaactacaaa gaatttattc tgggcaaagc cttaagttac ggcatcacct ggtttcgcga
    21001 tggcaacgga gaaattgtgg tggcttatgt cgagcgcaat tcccccgccg aaatagcagg
    21061 tgttgtgcgg ggctatcggg tggtgcagat ggatggcttt gctccagcag ccctcagcaa
    21121 tgagcagtta atagcggctg cgtaccccac cgagcagtcc cccgtccacc gctttgtatt
    21181 taaaaatagc cagggcgtgg agcagccgga ggtgcgttta agcgctgctg aagtgcaggt
    21241 taatagcgtg caaaatctgc agtatttcac cgccgccaac gagcgcaagg tagcttattt
    21301 ttccttcggc agttttaacg gcaccgggga aaaagaatta attaatacgg ttaatgagat
    21361 caaccaaacc ggcgtccaag aattaatttt ggatttgcgt tttaacggcg gcggttattt
    21421 agatttagcc tctgagcttg cctatatggt gggcggcgat ggggttaaaa aaacctccgt
    21481 gttttccgct ttgcgctttg cgccgggcaa tacagagtac agagatgaag tgattccatt
    21541 ttggcccacc agccgggccc taagcagcga tgtggccaaa gatcagccat tgccaacgct
    21601 caatttaaaa cgcctgtatg tgttgaccag cgccagcacc tgttcggcca gtgaatccct
    21661 gatcaatggc ctgcgggggg cggatttccc ggtggtgcag gtgggcacca ctacctgcgg
    21721 caaaccctac ggcatggtcc ccctctccaa ttgcggcttt gattattaca ccattaattt
    21781 ccgctacgaa aacgccaaag gctttagcga atttgaaagc ggcttagcgc cgcgcaattc
    21841 cgtgcagacc cagggcagcc ccctaggctg tgaggtggcc gaccagtacc gcgacccctt
    21901 aggcagcgcc aatgagtccc tgctggcggc ggccttaaaa catatcgaca gcggcagttg
    21961 cggctctgcg gcgctcgccg ccccgcgcgc gcagggcagc tccctgccac accccaaccc
    22021 tgtcttctcc aacgccatag gcccgcggct gagtttttag ccaatccggg cgcggcacgc
    22081 ctcaaaattc ccgcctaggg cttaaggaag tcccaattag aaagcagctg taggggcgcc
    22141 cctcgtgggt gccctctgcc cagtaagcgc atgctccatg gcagggggac taactgggca
    22201 accacaaggg ttgcccctac gatccggctt aatttaataa cacccttact cataacccaa
    22261 ttaacgtgcc cctacagggc cgatgggcaa aagccgacta tgcttaaggc aatgttaaat
    22321 ctgcttaggg cattagatgt tggggcggta tgcgtatttg cgaatagtac tgacgcggca
    22381 cgacaagtgg ctgctcggcg ctatcgcttt tagcctgctg tgcctgccat tgccccacct
    22441 gttacccatg cgggtgacct ctctgctgca ggcccaggtc agcctgcgct cctccattta
    22501 tgccgattcc agcgatcaag aggtgcagtg gctgaacaaa gaaaagctgc actggatctg
    22561 ccgggtgcgc gccgagtaca gcgccaaggc ctgcgggttt agcctggcct ttgacccgag
    22621 cctggactgg caagacgcct atggcctgcg cattgccctg gcggcccagt cgaagaacca
    22681 atttttccgc ctttatgtgc gcaaccatgt gccgggggcg gccggtacgc tgccggagga
    22741 tgcccagtac aaccaaatga tgctgccggc ccaggatgcc aaccagccgg tggatatagc
    22801 cttcagcgaa ttcgccctgg ccgactggtg gctggccagc cacccggtgg caaacgagta
    22861 caacatcgcc cgcttcgacc atgtcagcag cctagggatt gaccttgata gccccgccgc
    22921 cctcggcgat cacgaattgc agctggccca attggcgctg ctgcgcccct acatcagcgt
    22981 gcaaacctgt tacctaagcc tcttgggcct gtggacggcg ctgctgctgc tgttgagcct
    23041 gcagcgcctg tggctgcggc gcctgttgct ggcccaggcc cgggccaata tcgagagcca
    23101 gaagcctgcg gtggtggaat ttgaagagag cgcgccggcc ttttgcgaaa tcccccagga
    23161 tgatctcacc ggcatctaca accggcaggg ctttatcgat tattggagcc aggccagccg
    23221 cctctgggcg gataacgaca gcatgggcct gttggtggtg aacctggatc aatacaccag
    23281 catcggcgag agttttggcc gggatgcaaa agatcaagcc ctgctcgccc tggcggacgc
    23341 cctcaaaagc catatgcgcc cccaggataa attggcccgc tggcacgagg ggcagtttat
    23401 tttgctctgc cccctaaccg gccacgccca ggctctgcag ctggcggaaa agctgcgcca
    23461 ccacatcaac ctgatcagct ttgacgagcc tgccccccta tccttgagtg cctccatcgg
    23521 agtgattctc ttccccgccc aggccctttt agaacaggcc tataaccgct gcgaattggc
    23581 cctcaacaca accgcctcca acggcaatcg ggtgattgat tgcggggtac tcaaccttga
    23641 ctaagcggcc ctactttatc gccgcctggt tactaactgc catggcggtt atcgccctgg
    23701 cctgtttccc gctgctgccc aagcggtact ggaagctggg cgaggagagc cagttcaacc
    23761 ggctgttgat caccgataaa aacaaaggcg gccagaccga ggctaagtgg ctcggcgcgc
    23821 gacgctatca atgtgtgatt cagccgcaga ctcaagaccc cttctgcgcc atgcagatca
    23881 gccttaaaga cgaggccggc cggggccgcg acctaagcca gttcgaccgc ctgcgcatca
    23941 aggtgcggct gcaggggccg ggcagtgtgc tgcggctgca tatgcgggtg ttcgacgggc
    24001 gcttttccaa gccggggcag atggataccg ccaaattcca aggctatgtt tttagccccg
    24061 gtgagcaggg ccaggtgttg gagctgcccc tggcggggtt ttccgtggcg gactggtggg
    24121 tgcgggaatt cgcccccaag cgggaatatg cccagctgga ttttgccaat gtgttgatga
    24181 tcggcatcga tattcccgcc cccacccaag ccggcaccta cgatattgaa gtggaggagc
    24241 tggaatttat cggcagccgg gtacagctcg ccagtgttct ggtggtgttt atcctgctct
    24301 ggtgcgccct ggtggccgtg tatctgctgg cgggcatttg gcaggtggcg cggcagtggc
    24361 agcgggaact ccccgcctgg cgcgagc
//
LOCUS       DAOQGS010000003        20987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1176_length_20987_cov_5.317313, whole genome shotgun sequence.
ACCESSION   DAOQGS010000003 DAOQGS010000000
VERSION     DAOQGS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 20987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20987
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1176_length_20987_cov_5.317313"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..855
                     /locus_tag="PKZ52_00245"
     CDS             454..855
                     /locus_tag="PKZ52_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58221.1"
                     /translation="MHINIQSAFIARSRRFKAILKRYLRWVFARLGVPIECVNVKMRE
                     LTHEQKLVTSCRVHVQLKTGNSVVIEQHHLRASVAVIKAVRAAREALEVLFAQLNLGR
                     NGQRQLPYAQEPGHTTLRLKGVTHVLAINTY"
     gene            1370..3517
                     /locus_tag="PKZ52_00250"
     CDS             1370..3517
                     /locus_tag="PKZ52_00250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF010081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNG58222.1"
                     /translation="MPIARYTTAAGITVSRSAEAVCYDTAVDQLAQQLDSQLGCLLSS
                     SYEYPNRYKRWDMGFVNPMLSVTSRADRLWLEALNTRGEVVLAFLAEQLKGCAFIAEL
                     SLNPARCDLRIKPSAEVFTEEERSRQPSVFSVLRLLRECFFSSEDQHLGLYGALGYDL
                     AFQFEKIDFVLPRADDQRDMVLFLPDSIFIRDHERQEAFVYHYEFSLGGTSTEGLART
                     GQAIAYQPALSAPRECDHKPGEYAALVNTAKEYFARGDLFEVVPGQTFFLPCTDAPSA
                     VFIRLKHTNPSPYGFIMNLGQSEYLVGASPEMFVRVTDRLVETCPISGTIPRGTDAIS
                     DADQIRTLLNSDKDHNELTMCTDVDRNDKSRICVPGSVEVIGRRQIELYSRLIHTVDH
                     VRGTLREGYDALDAFLSHTWAVTVTGAPKFGAMTFLEHHEHSARRWYGGAVGKIGFNG
                     DINTGLTLRTIRIAGGIAEVRAGATLLSDSIPEAEEEETRVKASALIRALQGKAQTKA
                     QEASFVQQKGLGKKVLLIDHQDSFVHTLADYFRQTGASVTTLRYGFDESRLDAIKPDL
                     VVLSPGPGRPQDFALNQTIGQLIKRKIPIFGVCLGLQGLVEYFGGRLGVLDIPQHGRP
                     SEITVLTPNTGIFKGLELNRIQVGRYHSLYALADVLPADLTVTAITDDKIIMAIQHKT
                     LPIAAVQFHPESIMTFKNKVGITLVNNAYDLIG"
     gene            4044..5210
                     /locus_tag="PKZ52_00255"
     CDS             4044..5210
                     /locus_tag="PKZ52_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58223.1"
                     /translation="MNFKLRSCIILCGLVSVPSIADTLDVDAWITNAARPGLSKKIST
                     SGWSAFKKIAYLSASKSAKPSNIYLTPGNDIQATGIVVSPVPTTVTVDSYIAANCTSS
                     ALNVDKEISVQIADSSQVTMTTKLVTTGKVAGELKLPNGFGGSASLEKSVDITNQSEQ
                     KISKTRTMVDRIVKIVPPNTILSIDISSKEYPTFYDFKGNVIVDATILGFNFKDLVSN
                     NQIYVEGKVRGGTGQSITQIYRESSCAQSQFLASNPGKILYARPTASQLKSSSGVSNK
                     QTLELVKLKDQGLDYRSKSQDAIEIEQVLETSLKITPADIIGKIKFDATNNSEKLCTT
                     KIIANEIVVASFSLMPNENLGWKDLMSFIGSNPISIKTESDCESDMVYALKYPI"
     gene            complement(5477..5680)
                     /locus_tag="PKZ52_00260"
     CDS             complement(5477..5680)
                     /locus_tag="PKZ52_00260"
                     /inference="COORDINATES: protein motif:HMM:NF018748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1348 family protein"
                     /protein_id="HNG58224.1"
                     /translation="MAQRNVFLTRKWQMANGKWQMAHEYRLIKELWAFEDNRIAVRFA
                     YKWHDDSGQWYRSYGNENWEFDA"
     gene            complement(5928..6242)
                     /locus_tag="PKZ52_00265"
     CDS             complement(5928..6242)
                     /locus_tag="PKZ52_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HNG58225.1"
                     /translation="MKALGSCLMLSALLTSCAWVTPTTRGQAIAYLQPEQVQSCQKLG
                     RVSVETVNKVALMQRDAIKVQNELITMAKNEAVVLKADTIAPESEVKDGRQTFLAYRC
                     RN"
     gene            complement(6246..7283)
                     /locus_tag="PKZ52_00270"
     CDS             complement(6246..7283)
                     /locus_tag="PKZ52_00270"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG58226.1"
                     /translation="MAVIIEHTDPSGHVLHQQRFDGNQIIIGRSFSADMILTDPYVDA
                     EHCILSCDPDTGKLRLKDRGAKNGLWWLDHKRNKQRVISERVIESGQTVLLGKTYLRI
                     YHHLHPVPPALSIARDDAISGFFGHAGLILVVAILGLGLDILGRYLDHPIGGSWVERG
                     REAFYGLLGLVAVSSFWALLGKGLVQHSRFRFHFGLSLCVMAIWVVWEFIEPWLMYNF
                     NLSKTSADWLDRGLQALVLGGAFYLALSFSTRLSKRMRALLAGLIPLLILGSGVTSLF
                     EKKNRFRPVPAYSHVLVEPALQWRSPAPWDTLTGDAEKTYLPYDKHADDDDGIDDAAD
                     DSAESTTTAEE"
     gene            complement(7274..8542)
                     /locus_tag="PKZ52_00275"
     CDS             complement(7274..8542)
                     /locus_tag="PKZ52_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNG58227.1"
                     /translation="MTPLGMLWPVAWLLLLLPLGACAASAATADGVYSRYAPSLYQIR
                     IMENRSGSRAALGSGFVVNEGKFIATNYHVVSNKVMEPQKYRIEIDLAGKPLVANIVR
                     VDAVHDLALLALDGSPALGTALAIAKQVPQKGEVLYSLGNPHDIGMTVVVGNYNGLVE
                     HSPVEQIHFSGAINSGMSGGPTVNGDGQVIGVNVATAGNQIGFLVPGHYLQDLIEKAQ
                     EPLDKPLLEDMAEQIKAHTTALLDELLAQPWHPEPMGKVSIFAKAVPWVECWGNSDEK
                     KEQGTLEVSRGCNSNNQLFLSDKFSSGYVEYEFFYFSAPNWPSASVYRYMARNTANAM
                     PGNQVAKPHAGNYTCSNNWYTTPQGGSKRISYCTRPYKQLPGLFDVFYVGVTSDRSHE
                     VAMDHYTLSGVTQEASLRFLARFKEQMQWQ"
     gene            8914..9609
                     /locus_tag="PKZ52_00280"
     CDS             8914..9609
                     /locus_tag="PKZ52_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNG58228.1"
                     /translation="MAVVDGGLNLTQLVANALGQAIVQGKYQETSCLPTEAEISTEFG
                     TSRSVTREAVKMLTAKGLIASRPRQGIRVQSVQYWNLFDSEVLHWLQEARPSDALRRE
                     FAEMCWAIAPEAVSLACRHNDGEVLDSVSVALQNVEESQGNPQLWAQAHERFMGAFLK
                     ASGNRFFSQFEDFLRVSLRLHSNRINRLPHSIEPHRAICQALIEGRAQAAAAAMRRLM
                     AECFGWQFSPETA"
     gene            9921..11189
                     /locus_tag="PKZ52_00285"
     CDS             9921..11189
                     /locus_tag="PKZ52_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HNG58229.1"
                     /translation="MAGFSSTTSSVSGTAGQQNYTFALSALTTLFFMWGFLTCLNDIL
                     IPHLKEVFDLNYTQGMLIQFCFFGAYFLVSLPAGLLVKKVGYKTGAVIGLVIAAIGCL
                     MFYPAAALREYAVFLAALFVLAGGITVLQVSANPYVAILGKPETASSRLTLTQAFNSF
                     GTTIAPMIGGALILGAVTVDSNLTGDALLQAKAHAVQIPYVALASALLLLAAVFAFLK
                     LPVIVDEALPASTQDSGSAWQYSHLVLGAVGIFVYVGGEVAIGSLLVNFMGLPEIAGL
                     AEKDAANYVSYYWGGAMVGRFIGSAVMQKVPGGTVLAFNALAAVLLILVAVFTSGNVA
                     MWAILAVGLCNSVMFPTIFSLAVTGLGRHTSQGSSILCMAIVGGALIPLAQGALADTL
                     GLQLSFLLPILCYLYIVFYGLKGHKNTHTA"
     gene            11247..12620
                     /locus_tag="PKZ52_00290"
     CDS             11247..12620
                     /locus_tag="PKZ52_00290"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNG58230.1"
                     /translation="MKHLPLAPQQLFVLALVPAIIGLYWPTISVLAGRWLQWDKGLSH
                     GLLVAAVFIWLLWRRPSVTPPGGCWGLRLSLLGLASLLWFLMQSVQISLLAEGALLLV
                     IIAAYSAWLGFAQSWRYKAILLLPLFATSLWGEFNSAALSLSSVVVGQLVELAGITAH
                     LDGNTISLPYGQIIIADGCSGLRYMVIALALGHMLACLNHYPARGYWLTMAVALLLGL
                     ATNWLRIFVLVLVGYYTQMKSSLVADHETFGWLLFAGVVLPPLYFAPLRKAPSQAGSH
                     FAPPLGRLLAVAAVLALGPVLLALSQRPPSPAPWLAALPLKALPPPSWAIQTPMPQAG
                     LAEQGWQESVWLQRWQYQRVSSQDKLVPFIEANWHPDSQCKAVRTEALGAWFECSRFE
                     QKVLVLRRFDVGRFASADFRRAKLWQLPAQWLGDNRFSLSQWQQRCEASSCEQAAAQI
                     SALAAQP"
     gene            12668..13714
                     /gene="mutY"
                     /locus_tag="PKZ52_00295"
     CDS             12668..13714
                     /gene="mutY"
                     /locus_tag="PKZ52_00295"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNG58231.1"
                     /translation="MTQINKTRFRRAVLTWYQSHGRKHLPWQQNISPYRVWLSEVMLQ
                     QTQVETVIPYFERFIQRFPTVKALADAPIDEVLHLWTGLGYYARARNLHACAQQVAKL
                     GEFPSDLAALTALPGIGRSTAGAIRSLAFDLPAAILDGNVKRVLARVGAIAGWPGAPS
                     VAAELWALAEELAPRQNCRAYTQVMMDLGATLCKKSQPDCPRCPLKSQCQAYHAQSQA
                     LYPGKKPPKAVPVKTCYMLLARTKSGEILLQKRPAQGIWGGLWSLPEYPDEASLRQAC
                     LTHPCIKSLRQLKPLPPLTHRFSHYELIIQPLLASVTPTPAVQDSDAWLWYDRAAPPA
                     VGLAAPVKLLLEQN"
     gene            13735..14013
                     /locus_tag="PKZ52_00300"
     CDS             13735..14013
                     /locus_tag="PKZ52_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNG58232.1"
                     /translation="MSRLVFCRKYQAELPGLDAPPNPSPKGMALFESVSKQAWEEWMT
                     HQTRLINEKRLNLMDAAHRKYLAEQLEKFLNNEQIDQAEGYVPEPQAK"
     gene            14104..14179
                     /locus_tag="PKZ52_00305"
     tRNA            14104..14179
                     /locus_tag="PKZ52_00305"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14137..14139,aa:Phe,seq:gaa)
     gene            14400..16064
                     /gene="ettA"
                     /locus_tag="PKZ52_00310"
     CDS             14400..16064
                     /gene="ettA"
                     /locus_tag="PKZ52_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNG58233.1"
                     /translation="MAQYVYTMHRLGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSSLLRIMAGVDQDFIGEARPMPGIKIGYLPQEPQLDPAKDVRGNVEDGVREAVDALK
                     GLDEVYAAYAEPDADFDALAKRQGQLEDIIAAWDAHNLNHTLEVAADALRLPPWDADV
                     SKLSGGEKRRVALCRLLLSRPDMLLLDEPTNHLDAESVYWLEQFLLGFGGTVVAITHD
                     RYFLDNAAGWILELDRGQGIPYEGNYSTWLEAKEKRLEQEQRSEAAHQKALKAELEWV
                     RQNPKGRQAKSKARLARFEEMQSQEFQARNETNEIYIPPGERLGDKVIEFHNVSKAYG
                     DRVLMDKLSFTIPKGAIVGIVGGNGAGKSTLFRMITGQEKPDSGEIVIGETVNLAFVD
                     QSRDNLDNDKSVWEAVSGGADILKIGNYEIPSRAYLGRFNFKGTDQQKRVGDLSGGER
                     GRLHLADTLKKGANVLLLDEPSNDLDVETLRALEEAIAAFPGCALVISHDRWFLDRVA
                     THILAYEGDSEIVFFEGNYTEYHEDFIKRKGKDSQPKRVKYKPLKV"
     gene            16138..17322
                     /locus_tag="PKZ52_00315"
     CDS             16138..17322
                     /locus_tag="PKZ52_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG58234.1"
                     /translation="MIAKAELVALMADQESDRIERTESTNNTDKFSQAVCAFANDLPN
                     HQKPGYLVIGVDKRGIPTGIDVSEQLLENLGALRSNGNIQPLPFIVVAKFSLDGGDVA
                     VVEVHPSDLPPLRYKGQIWIRVGPRKATASEQEERILTEKRISRARSFDASPIAEANL
                     NDLAISQFHSYRQEVVDADVIIANHRSIEEQLASLRFFDSRHHVPTVGGLLLFGKNPR
                     FFLPGAYIQFLRLPGESLTDRPDDQAEISGDLLSVLREIEARIRANNSMRIEQASALR
                     EKNVFDYPEVAIREILLNAIMHRDYQSNTPVKLYWFSDRIEIHSPGGLYGEVTAETLI
                     RRSSYRNPVLAEAMKALGYVNRYGYGIQRAMSAMEKNGNNPIHFEMDDRTFLAVLPRI
                     SS"
     gene            17319..18305
                     /locus_tag="PKZ52_00320"
     CDS             17319..18305
                     /locus_tag="PKZ52_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG58235.1"
                     /translation="MKTVAFFNNKGGVGKTSLVYHLAWMYADQGIRTLAIDLDPQANL
                     SAMFLDEDRLSQLWEAASDATVMGSLLPLINRTGDIADPHVEKITEMLGLVCGNLALS
                     RFEDLLSENWPKCLDGKEDAFRVITAFHRIIERAAQLMSAQLILIDVGPNLGAINRSA
                     LIAAEQVVLPLAPDLFSIQGMKNLGPTLRDWRNGWKKRMSELPSSANISAPSGTMAPV
                     GYVVMQHGIRDSRPVKAYLRWMEKIPTVFRDSLLGEQEQLLTVKNDPYNLALLKHYRS
                     LMPMAMEANKPMFFLKPADGAIGSHVEAVRLCYQDFHALANVIASHAGVHMS"
     gene            complement(18413..19570)
                     /locus_tag="PKZ52_00325"
     CDS             complement(18413..19570)
                     /locus_tag="PKZ52_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58236.1"
                     /translation="MSISAICLGLCQFLFMASVAVGIAFNSLVGKQLAPSPSLATLPF
                     LGMMGATAALTLFMPKGLSLLGYRGVFILGAAVGALGCALATYSIWFEEFALFCIAGA
                     LIGVYQASALYYRFAAADAVDGPLKSTAIAWVLSGGIAAALIGPMIGKNFLHSLPVEY
                     TGAYLAAMVLCLLAIPVFAFAPLPKRQAASNTAVTWWPLPRIAVQAMLFCTVGYCLML
                     MVMLASPLAMAGCGFHAGDAASVIQWHLLGMFAPSLMTGKWIARWGAPSVAWAGVAIL
                     AAGCAVALASTSLIAFHAALFVVGVGWNFMYMGGSTLLTQIPDAPLRARLQAINEFCT
                     FTSVTLASGLTGWVYALLGWQAILIMGIMLLAGLFAAQWGRRPVVQVQVQA"
     gene            complement(19784..20593)
                     /locus_tag="PKZ52_00330"
     CDS             complement(19784..20593)
                     /locus_tag="PKZ52_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58237.1"
                     /translation="MASRAHVVYAPSLTSEETRQRCCPSGCWQQIASPLQKLAISPAL
                     SFGIPTAIGGLRILLLGEGNGQEVFCLAHQAGPAGEVFAVTPNQTTLELWRQWQSEFC
                     QREGFDNLSLLYSGSQSLSQLPLAAASFDLIISLESKVHSLLFDTPYLARLLKTGGEW
                     LRITARQPTRRPAGFSGPFSLASQVVGNHYRATRWFKRAVDASDTAPVRLCYTGGIED
                     CPDELVFDQYHLKTGQFTALPDDQYQVLINSRYRDYLLPEQKAPGGGLFSD"
     gene            20735..>20987
                     /locus_tag="PKZ52_00335"
     CDS             20735..>20987
                     /locus_tag="PKZ52_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNG58238.1"
                     /translation="MLKLSEVLNRFNLSAQVFYAGDVCGTLAFEQASPLEGHMHLIKQ
                     GRLDIRAGDAWAYVIERPSLVFMARSQQHQLRAQVEAGAQ"
BASE COUNT         4830 a         5400 c         5654 g         5103 t
ORIGIN      
        1 aatggccgac aaggtggccc tagcggtgaa ccaggccctc gcctatgtcg atgcgcgcac
       61 cggcactcaa aaccaaaatc cccccgccga cgtagaagcc gcgtcggcat aatttgccgt
      121 agcctcatag tgtttcgcca tcacagcttt gcgcgccgat aaaaaatttt ttcggctgcg
      181 taattgttcg attttacaca ccaatttgcg acaccattgg caaaaatttc ccagtgcaag
      241 gctttatcgc agggcctaaa tgaaactaat tttttattcg ctactcccaa agccactcgc
      301 gcagattagg taaaagcctt ttatgtgttg agtgattatc gaaaatgggt ttttcgatac
      361 ctctaaaaaa cctgcgcacg atacaaaaat attcgatttg ttgagagagt tttacttgtc
      421 tagtatgcct aaaaaccatc tggagacctc gccatgcata ttaatattca aagtgctttt
      481 atcgctcgct cccgccgttt taaagcgatt ttaaagcggt atttacgctg ggtatttgca
      541 cgcttaggtg tgcccatcga gtgtgtaaat gtgaagatgc gtgaattaac gcacgagcaa
      601 aaattagtaa ccagttgccg cgtgcatgta cagcttaaaa ccggcaacag tgttgttatc
      661 gagcagcacc atctgcgcgc ctctgtggcg gtaattaaag cggtgcgcgc cgctcgcgaa
      721 gccttagagg ttttatttgc ccagcttaat ttaggccgta acggccagcg gcagttgccc
      781 tacgcgcagg agcctggcca cacaaccctg cggctaaaag gcgtgaccca cgtgttggcc
      841 attaatactt attaacccgg cggcaaaata gatcgtcctg atctattttg ccttcgctgc
      901 actaaaactg gcggaaatgc gtggatttcc ttgttttagt gcagctcgcg gcggtttgca
      961 ccgccaaggc ggcattcatg ccgccattat taacccggca ctatttgccg ccgggttaat
     1021 aatgggctgc accaatcgtt gtataaaaat tgtcggtcac tcaatacagt ttccttatgt
     1081 aaatcgcgct cggtgttcag ttgattaaag tgatttaaca ttcagaattt tttgcgcagg
     1141 tggccgacaa atttaattcc ccttggcgtt ggggttgcgt atttaattcg tcagatgctt
     1201 aactcggctt gccgatacca cttaattgtt ttgctcgata gtggcaagca ccccgctaaa
     1261 tgctgcttca gtagtgccat cctttgaaaa acccccgcgc cgcaggcata aacccatgcc
     1321 ataataggcc agccccagaa cggggatttc gccctatcga gaccacacaa tgccaattgc
     1381 ccgctacacc acagctgccg gtattacggt aagccgcagc gcagaagccg tctgttacga
     1441 caccgccgtc gatcaactcg cccagcaact cgacagccaa ttaggctgcc tcttgtcctc
     1501 cagttacgaa taccccaacc gctacaagcg ttgggacatg gggtttgtaa accccatgct
     1561 gagcgtcacc agccgagccg atcgcctctg gcttgaagcc ttaaatacac ggggtgaagt
     1621 ggtattggca tttttggccg agcagttaaa gggctgtgca tttattgccg agctaagcct
     1681 aaatcctgcg cgctgcgacc tgcgcattaa acctagcgcc gaggtattta ccgaagaaga
     1741 gcgcagccgt cagccatccg tatttagtgt gttgcgcctg ttgcgggagt gtttttttag
     1801 cagcgaagat caacacctgg gcctctacgg cgccctgggc tacgacctgg ccttccagtt
     1861 tgaaaaaatc gactttgtgc tgccccgtgc agatgatcag cgggatatgg tgttatttct
     1921 gcccgacagt atttttattc gcgaccacga gcgccaggaa gcctttgttt accactatga
     1981 attttcctta ggcggcacca gcaccgaagg cctcgcccgc accggccagg caattgccta
     2041 tcagcccgcc ttaagtgcac cccgcgaatg tgaccacaaa cccggtgaat atgccgcttt
     2101 agtcaatacc gccaaagagt atttcgcccg tggcgattta tttgaagtgg tgcccggcca
     2161 aaccttcttt ttaccctgta ccgatgcccc cagcgcggta tttattcgct taaaacacac
     2221 caatccatcg ccctacggtt ttattatgaa tttggggcag agcgaatatt tagtgggcgc
     2281 ctcgccggaa atgtttgtgc gcgtcaccga ccgcttagtg gaaacctgcc ccatctccgg
     2341 cactattccc cgtggcaccg atgccattag cgatgcggat caaattcgca ccctgctcaa
     2401 ctccgataaa gaccacaacg aactcaccat gtgtacggat gtggaccgca acgataaatc
     2461 tcgcatctgt gtgccaggct ccgttgaagt gattgggcgg cggcaaattg aattgtattc
     2521 ccgcctgatt cacaccgttg accatgtgcg cggcacttta cgggaaggct acgacgcctt
     2581 ggacgcattt ttaagccaca cctgggcggt caccgtaacc ggtgcgccca aattcggcgc
     2641 catgacgttt ttggaacacc acgaacacag cgcccgccgc tggtacggcg gcgccgtagg
     2701 caaaataggc tttaacggcg atatcaacac cggcctcacc ctgcgcacta ttcgcatcgc
     2761 aggcggtata gcggaagtgc gggcaggggc cacgttgctc agcgactcta tccccgaggc
     2821 ggaagaagaa gaaacccgcg ttaaggcctc ggccttaatt cgcgccctgc aaggcaaagc
     2881 tcaaaccaag gcgcaagaag ctagcttcgt gcagcaaaaa ggcctgggta aaaaagtgtt
     2941 attaatcgac catcaagaca gttttgtgca caccctggcc gattatttcc gccaaaccgg
     3001 cgccagcgta accaccctgc gctacgggtt tgatgagtcc cgtttagacg ccataaagcc
     3061 cgatttagtc gtgctctccc caggccccgg ccgcccccag gattttgccc ttaaccaaac
     3121 catcggccaa ttaattaaac gtaaaattcc aattttcggt gtgtgtttag gcctgcaagg
     3181 gttagtggaa tatttcggcg gccgcttagg cgtattagat attccccaac acggccgccc
     3241 ttcggagatt accgtgttaa cacctaatac ggggattttt aaaggtttgg aattaaatcg
     3301 catccaggtg ggccgctatc attccctgta cgcccttgcc gatgtgttgc ctgcggattt
     3361 aactgtaacg gcgattacgg atgataaaat tattatggcc atccagcata aaactctgcc
     3421 aattgccgct gtgcaatttc acccagaatc catcatgact tttaaaaata aagtggggat
     3481 tacgttggtg aataatgctt atgatttaat tgggtgaatg gattcccatg gatcgaggtg
     3541 agcaaaattt ttagggaacc tctgaaatat tactttactc gccctggcgg tgttaaattt
     3601 gccaaaaaaa tgctcatcta ctacatgtaa actccgcttt ttttggcaaa ttcgcctagc
     3661 cagtgcttcg ccgcctacat ttttcagagg ttccttaaca tatctcgtat gattcttttt
     3721 ttaacttgat atttaactga tagtgcgaag agcttatgct taaaaggagt tgagtcgggc
     3781 caattgtcat ggataatgtg caatcaatat gcaatgccgc ggtggatcaa tgagaaatac
     3841 gcaaggaata agaaggggcg tatagatttt gagtttcgag tgcaaatttt atctattttt
     3901 gctgatttta tgtgcaaaaa ctcttgacca gaccggatct tataaattaa gatgcctccg
     3961 cctggtggca attacataac aagggaaatt tgtgaggaat gcgagttttc cttgttttct
     4021 cattattata tacggagcct aaaatgaatt ttaaactaag atcttgcatt attttatgtg
     4081 gcctggtgtc agtgccgtcg attgcggata cccttgatgt agatgcttgg attacaaatg
     4141 ccgctcgtcc gggtttgagc aagaaaatat ccacgtctgg ctggagcgct tttaaaaaaa
     4201 ttgcttacct ttcggcatcc aaatcagcaa agccgtccaa tatttatttg acccccggca
     4261 atgatataca agctacgggc attgttgtgt cgccagtccc aaccactgta acggttgatt
     4321 cctatattgc agcaaattgc acatcgagcg cattaaatgt tgacaaagaa atatctgtcc
     4381 aaatcgccga ttcatcacag gtgacaatga caacaaagtt ggtcaccacc ggcaaagtag
     4441 caggcgagct gaagctgccg aatggttttg gggggagcgc ttcactggaa aagagcgttg
     4501 atataacaaa tcaatcagag cagaaaataa gtaaaactag gacaatggtt gatagaatag
     4561 taaaaatcgt accacccaac acaatattat caattgatat atcaagcaaa gagtatccca
     4621 ccttttatga ttttaaagga aacgtaattg ttgacgctac aattttgggg tttaacttca
     4681 aggacctagt ttctaataat caaatttatg tagaaggtaa agttagaggc ggtacagggc
     4741 aatccatcac tcaaatatat cgagagtcat cttgcgccca aagccaattc ttagctagca
     4801 atcccgggaa gattttgtat gctcgtccta cagcgtcgca actcaaaagc tcatcggggg
     4861 tgtcgaacaa gcaaacatta gaattggtga aactgaagga tcaagggtta gattatagat
     4921 ccaaatctca ggacgcgatt gaaattgagc aagttttaga aacctcgctt aagataaccc
     4981 ctgctgatat tattggaaaa ataaaattcg acgctacaaa taatagcgaa aaattgtgca
     5041 ctacaaaaat aatcgcaaac gaaattgttg ttgcaagctt cagtctaatg ccaaacgaaa
     5101 acttaggctg gaaggatttg atgagcttta taggttctaa tcctatatca attaagaccg
     5161 aatcagattg cgaatcagat atggtgtacg ctctaaaata ccccatttaa tttttggcgt
     5221 cattacggtg gcatattttt tatcaatgtc gatttgaaat cgagcaattt aaccggattt
     5281 gtaacaagga tgttgcaaat tttctctgat gggctttcaa aaaaagtacc ccgagcatta
     5341 ctttaaactt tttacaatcc caactcactc aaccccgccg catccgccgg ccttggcccc
     5401 tggggccagc gaaacttgcg ctcgtgttcg gcaatgacta aatcattaat actagcgtag
     5461 agatttgcat taaaccctag gcatcaaatt cccagttttc attgccatag gagcggtacc
     5521 actggccgct gtcatcgtgc catttgtagg caaaacgcac cgcaattcga ttgtcctcaa
     5581 aggcccataa ttcttttatt aggcgatatt cgtgtgccat ttgccatttg ccatttgcca
     5641 tttgccattt gcgggttaaa aatacattgc gttgcgccac ttgctgtcgg gggtataagt
     5701 ctgggaaacc ttttcagggt tgcgcgcatt ccagccatct tcggcggctc gtaccttttg
     5761 catggtggtg gcgagggttt agggcggtag ggggcgtggt gttgtcatag gattctccga
     5821 acgtttaccc ctgcgggcgg cggcatgaaa ccaattgtga gcgggcttag gccttatcca
     5881 tttagtactc aacccgatca cgtctggctt attacaggga agagtggcta gttacggcag
     5941 cgataggcca aaaaggtttg gcggccgtct ttcacttcgc tctccggtgc aatggtgtcc
     6001 gcctttaata ccaccgcttc atttttggcc atggtaatta attcgttttg cacttttatc
     6061 gcgtcccgct gcataagcgc aactttattc acggtttcga cggatacgcg gccgagtttt
     6121 tggcagcttt gcacttgctc cggctgtaaa taggcaatgg cctgcccgcg ggtggtgggg
     6181 gttacccagg cgcagctggt tagaagtgcg ctgagcatta aacaagaacc taaagctttc
     6241 atgggttact cctctgcagt ggtggtcgat tcggcgctgt cgtctgcggc atcgtcaatg
     6301 ccatcatcgt catcggcgtg tttatcgtag ggtaggtaag ttttctcggc atcgcccgtc
     6361 agggtgtccc agggggctgg gctgcgccac tgtagggccg gttccaccag cacatggctg
     6421 taggcgggca ccgggcggaa gcggtttttc ttttcaaata ggcttgtaac gcctgagccg
     6481 agtattaata gagggatgag cccagctaaa agtgcccgca tgcgtttaga aagccgggtg
     6541 ctaaagctca gtgccaggta aaaagcgccg cccagcacta gcgcttgcag gccccggtcg
     6601 agccaatcgg cgctggtttt actgaggttg aagttgtaca tcagccaggg ttcgataaat
     6661 tcccacacca cccaaatagc catcacacac agggacagcc caaaatgaaa acgaaagcgg
     6721 gagtgttgca ccaggccttt gccgagcagg gcccaaaagc tgctcacggc cactagccct
     6781 aacaggccgt aaaaggcctc gcgcccgcgc tctacccagc tgccgccgat gggatggtct
     6841 aaatagcggc ccaagatgtc tagccccaag cccagaatgg ccaccaccaa aataagcccg
     6901 gcatggccga aaaacccgct gatggcgtcg tcccgggcga tggagagggc aggaggtacc
     6961 gggtgcaggt ggtggtagat gcgcaaatag gttttgccca gcagaaccgt ttggccagac
     7021 tctatgactc gctcgctgat cacccgctgt ttattgcgct tgtgatccag ccaccagagg
     7081 ccgtttttgg cgccgcgatc cttaaggcgc agttttccgg tgtccgggtc gcagctgagg
     7141 atgcagtgtt cggcatctac ataggggtcg gtgagtatca tgtcggcgct gaagctgcgg
     7201 ccgatgataa tttggttgcc gtcaaagcgc tgttggtgca gcacatggcc gctagggtcg
     7261 gtgtgttcga tgattactgc cactgcattt gctccttaaa gcgcgccaaa aaccgcagcg
     7321 aggcctcttg ggtgacgccg gataaggtgt agtgatccat cgccacctcg tggctgcgat
     7381 cgctggtgac acccacgtaa aacacatcaa acaggcccgg taattgttta taggggcggg
     7441 tgcagtagct gatgcgtttg ctgccgcctt gaggggtggt gtaccagttg ttgctgcagg
     7501 tgtaattgcc cgcgtggggt ttggccacct ggttgccggg catggcgttg gcggtattgc
     7561 gggccatata gcggtagacg ctggcggagg gccagttggg ggcagagaaa taaaagaact
     7621 cgtactccac ataaccgctg gagaatttat cgcttaaaaa caattggttg ttggagttac
     7681 agccgcggga tacctccagg gtgccctgtt cttttttctc gtcgctattg ccccagcact
     7741 ctacccaggg cacggctttg gcgaagatgg ataccttacc catgggctct gggtgccagg
     7801 gttgggcgag cagctcatct aacagggccg tagtgtgggc ttttatctgc tcggccatat
     7861 cctccagcag gggtttgtct aggggttctt gggctttttc gatgaggtcc tgtaggtagt
     7921 ggccgggcac caaaaaaccg atctgattac cggcggtggc cacgttcacg ccgatcactt
     7981 gtccgtcgcc attgacagtg gggccgccgc tcatgcctga gttgatggcg ccgctaaagt
     8041 gaatttgctc cacagggctg tgttccacca ggccgttgta gttacctacc actacagtca
     8101 tgccgatatc gtgggggttg cctagggagt agagcacctc gcccttttgc ggcacttgtt
     8161 tggctatggc cagggccgtc cctaaggccg ggctgccatc tagggcgagc agggccaaat
     8221 cgtgcaccgc atccactcgc actatgttgg ccaccagggg tttgcccgcc aggtcaattt
     8281 caatgcggta tttttgtggt tccatcacct tgttggatac cacatggtag ttggtggcga
     8341 tgaatttccc ctcgttcacc acaaaccccg agcccagggc cgcccggctg ccagagcggt
     8401 tttccatgat gcgaatttgg tagagggagg gggcgtagcg ggagtaaacc ccatcggcgg
     8461 tggccgcgct tgcagcgcac gcgcctaggg gcagtaacag gagtagccag gcaactggcc
     8521 acagcatgcc aagtggtgtc atagaagagg ggccttgcgt cgaaaatgct gcgcagcata
     8581 gcgcgaaatc catcggcaat cctaaacctt aaaaggttcc gtcaattaca ggtcgctgcc
     8641 ggtgcggcca agcctctgtt gatcggccgt tcactcctat ttgacgtatt tgaaacccgc
     8701 cgcctgtctg gcaaataggg ttgtttgtcc cggcgcggcc aaaatataag catatagtac
     8761 gataattttg atatgatcac tgttacgcaa aaatacataa tttaagtcag acttatgtaa
     8821 cactttgcaa agaattatag ttaaaatgac actactaaga ttattcctag tagggtcgtt
     8881 gctgcccgtg tcagctgaat gtagaggtat tgtatggcgg tagttgatgg tgggcttaac
     8941 ctgacacagc ttgtggctaa tgcgttggga caagctattg tgcagggtaa atatcaggag
     9001 accagttgcc tccccacaga ggctgaaatc tccacggaat ttggcactag ccgcagtgtt
     9061 acccgcgaag cggtgaagat gctgacggcc aaaggcttga ttgcgtcaag gccccggcag
     9121 ggtatccggg tgcagtcggt gcagtactgg aatctgtttg attcagaggt gttacattgg
     9181 ctgcaggagg cccgccccag cgatgcattg cggcgggaat ttgccgagat gtgttgggcc
     9241 atagcgccgg aagcggtctc tttagcctgc cgccacaatg atggtgaggt gctcgatagc
     9301 gtttcggtgg cgctgcaaaa cgtcgaggag tcccagggca atccgcagtt gtgggcccag
     9361 gcccatgagc gttttatggg ggcgttttta aaagccagcg gcaaccgttt tttctcgcaa
     9421 tttgaagact ttctgcgggt ttccctgcgt ttacacagca accgcatcaa tcgtttgccc
     9481 cactctattg aaccccatcg ggccatttgc caggcgctta tcgaaggccg cgcccaggcg
     9541 gcggcagcgg ccatgcggcg cttgatggcg gagtgtttcg gttggcaatt cagccctgaa
     9601 accgcctaaa ccctgcgcac aaatgacact gcaccccatg tggtgcaggt gtttgcacag
     9661 gttacggcgc tagcactgaa gttgtgaaac attttcatac tggcgcccct gcaacacgac
     9721 acgctgaagc aaccatcaca caattaggcg tagctcccgc ccaggggaat gcgcataata
     9781 ccccgcccgc tttaacagcc atcaccttct gctgcccgcc caggccgcct gctgatggca
     9841 ctgttgaagt agacggcacg ctccacgggg cccaagcagt gggccgcttt tcactcaaac
     9901 aataataaag aggcgtatct atggctggat tttcttccac cacgagctca gtcagcggca
     9961 cagcaggcca acaaaactac acctttgccc tgtcggcgtt gaccactttg ttttttatgt
    10021 gggggttttt aacctgcctg aatgacatcc tgatcccgca tttaaaagag gtgtttgatt
    10081 taaattacac ccagggcatg ttgattcagt tttgtttctt cggcgcctac tttttggtgt
    10141 ccctgcccgc cggcctgttg gtaaaaaaag tcggctataa aaccggcgcg gtgattggct
    10201 tggtgattgc ggccatcggc tgcttgatgt tttaccccgc cgctgccctg cgcgagtacg
    10261 ctgtattttt agcggcgctg tttgtgctgg ccggtggtat caccgtgtta caagtctccg
    10321 ccaaccccta tgtggcgatt ttgggtaaac ccgaaaccgc ctccagccgc ttaactctta
    10381 cccaggcctt taactccttc ggcaccacca tcgcccccat gattggcggt gcgttaattt
    10441 tgggcgccgt gacggtggac tccaacctca ctggcgatgc tctgttgcaa gctaaggccc
    10501 atgcggtgca aatcccctat gtggccctgg cctctgccct gttgctgctg gcggcagtgt
    10561 ttgcgttttt gaaattgccg gtgattgtgg atgaggccct gcccgcttcg acacaggatt
    10621 ccggctccgc ctggcagtac agccatttgg tgctcggcgc cgtggggatt tttgtgtatg
    10681 tgggcggcga ggtagccatc ggcagcctgt tggtgaactt tatgggcctg ccggaaattg
    10741 ccggcctggc tgaaaaagac gccgctaact acgtcagcta ctactggggc ggcgccatgg
    10801 tgggccgctt tatcggttcg gcagtgatgc aaaaagtgcc cggcggcaca gtattggcct
    10861 ttaatgcctt ggcggcggtg ctgttgattc tggttgcggt gttcacctcg ggcaatgttg
    10921 ctatgtgggc gattttggcg gttggcctgt gtaattcggt gatgttcccc accattttta
    10981 gcttggcggt gaccggcctt ggccgccaca ccagccaggg ttcgtccatt ttgtgtatgg
    11041 cgattgtggg cggcgcctta attcccctcg cccagggcgc cctggccgat acgcttggcc
    11101 tgcagctgtc ctttttactg cctatcctgt gttatctcta catagtgttt tacgggctta
    11161 agggccataa aaacacccac accgcttagc caggcattac tcagcagtgc cgtcgaggcc
    11221 ctgctgagcc gcccttttaa ttgcccatga aacacctgcc gcttgcgcct caacagctgt
    11281 ttgtcctcgc cttggtgccc gccattatcg ggctctattg gcccaccatc agtgtgttgg
    11341 cggggcgctg gctgcagtgg gataagggcc tctcccacgg gttgctggtg gcggcggtgt
    11401 ttatctggct gctgtggcgc aggccgagtg ttacgccgcc gggcggctgt tgggggctgc
    11461 gcttaagcct tttggggctt gcgtccctgc tgtggtttct catgcagtcg gtgcaaatta
    11521 gtctgctggc ggaaggcgct ttgctgctgg tgataattgc agcttacagc gcgtggctcg
    11581 gttttgccca gagctggcgc tataaggcga ttttgctgtt acctctcttt gccaccagcc
    11641 tgtgggggga atttaactcg gcagcgctgt cgttgagcag tgttgttgtc ggccagctgg
    11701 ttgaactggc gggcattacg gcgcacctgg atggcaacac catcagcctg ccctacgggc
    11761 aaattattat tgccgatggc tgctcgggcc ttaggtatat ggtgattgcc ctggctctgg
    11821 ggcacatgtt ggcctgtttg aatcattacc ctgcgcgggg ctattggctc accatggctg
    11881 tggcgctgtt gttggggctg gcgaccaact ggctgcgtat ttttgtgctg gtattagtgg
    11941 gctactacac ccaaatgaaa agtagcctag ttgccgacca tgaaaccttc ggttggctgc
    12001 tatttgccgg tgtggtgttg ccgccgctgt attttgcccc cctgcgtaaa gcgccgagcc
    12061 aagcaggctc tcactttgcg ccacctttgg ggcggctgct ggcggtggct gcggtgctgg
    12121 ccctgggccc tgtattactt gcgctcagcc agcgcccacc tagccccgcc ccttggttgg
    12181 ctgcgctacc cctcaaggct ttgccgccgc cgagctgggc aatccaaact cctatgcccc
    12241 aggcaggcct tgcggagcag ggttggcagg agtctgtgtg gctgcagcgc tggcagtatc
    12301 aacgggtaag cagccaggac aaactcgtgc cgtttatcga ggccaattgg caccctgata
    12361 gccagtgtaa ggctgtgcgc actgaggcat tgggcgcctg gtttgagtgc agccgctttg
    12421 agcaaaaggt gttagtcctg cgccgctttg acgtgggccg ttttgccagc gctgactttc
    12481 gccgcgccaa actctggcaa ctgcccgccc agtggctggg ggataatcgc tttagtctca
    12541 gccagtggca gcagcgctgt gaggccagca gctgcgagca ggccgcagct caaatcagcg
    12601 ctcttgccgc ccagccctaa tcgccgttca aagcgtataa cccggcctcg accgccagct
    12661 gatccccatg acacagataa acaaaacccg ctttcgccgc gccgtcctca cctggtacca
    12721 aagccacggc cgcaagcatt tgccctggca gcagaatatc agcccctacc gggtgtggct
    12781 gtcggaagtc atgctgcagc aaacccaagt ggaaaccgta attccctatt tcgagcggtt
    12841 tatacagcgc tttcccaccg ttaaagccct ggccgatgca cccattgatg aggtgttaca
    12901 cctgtggacc gggttgggtt attacgcccg cgcccgcaac ctgcacgcct gcgcccagca
    12961 ggtggcaaaa ctcggcgaat tccccagtga cttagcggcc ctaaccgctc ttcccggcat
    13021 tggccgctcc accgcaggcg ccattcgcag cctggcgttt gatttaccgg cggcgattct
    13081 cgatggcaat gtcaaacggg tgttggcgcg ggtgggggcc attgccggtt ggcccggcgc
    13141 gcctagtgta gcggccgagc tgtgggctct ggcggaggag cttgcccctc gccaaaactg
    13201 ccgcgcctat acccaggtga tgatggattt aggcgctacc ctgtgcaaaa aatcccagcc
    13261 ggactgcccc cgctgccccc ttaaaagcca gtgccaggcc tatcacgccc agagccaagc
    13321 cctctaccca ggcaaaaaac cgcccaaagc cgtgccggtg aaaacctgtt acatgctgct
    13381 ggcacgcacg aagagcggtg aaattctgct gcaaaagcgc ccggcccagg gcatttgggg
    13441 cgggctgtgg agcctgcctg aataccccga tgaggcctcc ttgcggcagg cttgcctaac
    13501 tcacccctgc attaaaagcc tgcgccagtt aaaacctctg ccgcccctca cccaccgctt
    13561 tagccactat gaattaatta ttcagccgct gttggccagc gtcaccccaa cgcctgccgt
    13621 gcaggatagc gatgcctggt tgtggtacga tcgcgccgcg ccgcccgccg ttgggctggc
    13681 cgctccggtc aaactgctgc tggagcaaaa ctgatgttgg attgaggagc cgccatgagc
    13741 cgccttgttt tttgccgtaa ataccaagct gaactacccg gtttagacgc cccccccaac
    13801 cccagcccca agggcatggc cctgtttgaa tccgtctcca aacaggcctg ggaggaatgg
    13861 atgactcacc aaacccgcct cattaatgaa aagcgcctca acctcatgga tgcggcccac
    13921 cgcaagtatc tggccgagca attagagaaa ttcctaaata acgagcagat tgatcaagcc
    13981 gaaggctatg tgcctgaacc acaagcaaaa taatttttaa agcgaataga aaatccccgc
    14041 tcttcccttg acgagggcac tgcaagcggg tttaatacgc gcctcgctaa agcagcactt
    14101 agcgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt cggtggttcg
    14161 attccgcctc cgggcaccat aattcaaagg cctagaagaa attctaggcc ttttttattg
    14221 cgtctatttt ttgcctgcgc gcgattattt ttagcggtaa ttgtgttttt taaacctgtt
    14281 tttcaccgcg ttgtttaaac tcagccgagc atttgaaatt accccgccgc aaggctaacc
    14341 ttcgcgccgt cctttctttt tcatccccat taaacccact aaattcgcag gatccttcca
    14401 tggctcaata cgtctatacc atgcaccggc tcggcaaaat cgtgccgcct aagcgccaaa
    14461 ttttaaaaga tatttctcta tcgtttttcc ccggcgccaa gattggtgtg ttgggtttaa
    14521 acggttcggg taaatccagc ctgctgcgca ttatggccgg ggtggatcag gattttatcg
    14581 gcgaggcgcg ccccatgccg ggcattaaaa ttggctactt accccaagag ccccagctgg
    14641 accccgccaa agatgtgcgt ggcaatgtgg aagacggcgt gcgcgaagcg gtggacgcat
    14701 taaaaggcct ggacgaagtc tatgccgcct acgccgaacc cgatgcagat tttgatgctc
    14761 tggccaaacg ccaaggtcaa ttagaagaca tcatcgccgc ttgggatgcc cacaatctca
    14821 accacacctt ggaagttgcc gccgacgccc tgcgcttacc gccatgggat gcggatgtaa
    14881 gcaaactctc cggcggtgaa aaacgccgcg tggccctgtg ccgtttattg ctctcgcgcc
    14941 cggatatgtt gttattggac gaaccgacca accatttgga tgccgaatcg gtgtattggc
    15001 tggagcaatt tttattgggc tttggcggca ccgtggtggc catcacccac gatcgctact
    15061 ttttagataa tgccgccggc tggattttag aactcgaccg cggccagggt attccctacg
    15121 agggcaatta ttccacttgg ctggaagcca aagaaaaacg cctagagcaa gaacagcgct
    15181 cggaagcggc gcaccaaaaa gccttaaaag ccgaattaga gtgggtgcgg caaaacccca
    15241 aaggccgtca agccaaaagc aaagcgcgtt tggcgcgttt tgaagaaatg caaagccaag
    15301 aattccaagc ccgcaatgaa accaacgaaa tatatattcc accgggtgag cgcttggggg
    15361 ataaggttat tgaatttcac aatgtcagca aagcctatgg cgatcgcgtg ttgatggata
    15421 aattaagttt taccatccct aaaggcgcca ttgtgggaat tgtcggcggt aatggtgcgg
    15481 gtaaatcgac tttatttcgc atgatcaccg gccaggaaaa acccgatagc ggtgaaattg
    15541 taatcggtga aacggttaat ttagcgtttg tagatcagag ccgggataat ttagataacg
    15601 ataaaagtgt ctgggaagcc gtctccggcg gcgccgatat tttaaaaatt ggcaactacg
    15661 aaatccccag ccgcgcttat ttaggccgct ttaattttaa aggcaccgac cagcaaaaac
    15721 gcgtggggga tttatccggt ggtgagcgcg gccgtttaca cctagccgac actttgaaaa
    15781 aaggcgccaa cgtattatta ctcgacgaac cctccaacga tttggacgtg gaaaccctgc
    15841 gcgccttaga agaagctata gccgccttcc ccggctgcgc gctggttatt tcccatgacc
    15901 gctggttttt agatagggtg gcgacacata ttctggccta tgaaggcgat agtgaaattg
    15961 tgttttttga ggggaattat actgagtacc atgaggactt tattaagcgt aaggggaagg
    16021 attctcagcc taagcgggtt aagtataagc cgttgaaggt ttaggttttt tgtggcggcc
    16081 cttatgggcc gttaatgcag taagatgtgg tgagaaaatt aattttttgg aggaattatg
    16141 atcgctaagg cggagttagt tgcattaatg gccgatcagg agagtgatag aatagagcga
    16201 acggagtcca ccaacaatac ggataaattc tcccaggccg tttgcgcttt tgcgaatgat
    16261 ttacctaatc atcaaaagcc aggctatttg gtaatcggag tcgataaacg aggcatccct
    16321 acaggtatag acgtaagcga gcaattattg gaaaacctag gggcgcttcg ttcaaatggc
    16381 aatattcaac cgctgccgtt tatagttgtt gcgaaatttt ctcttgatgg tggtgatgta
    16441 gctgttgttg aggttcaccc ctctgacctt cctccgctgc gttacaaagg ccaaatatgg
    16501 attcgtgtag ggccacgtaa ggccacggct agcgaacagg aggagcgaat tttaacggag
    16561 aaacgtatct ccagagcacg tagcttcgat gcaagcccca tagccgaagc caatttaaat
    16621 gatttagcca tttctcaatt ccactcctac aggcaagagg ttgtagacgc agatgtaatt
    16681 attgccaacc atcgctctat cgaagagcaa ttggcctctc tgcgtttttt tgatagccgt
    16741 catcacgtgc cgacggttgg cggtctatta ctttttggca aaaatcctcg attttttttg
    16801 ccgggggcgt atatccaatt tttgcgcctg ccaggagaat cactaacgga tcgcccggat
    16861 gatcaagcag aaatctctgg cgatttgcta agcgtattgc gagaaataga agcaaggata
    16921 agagctaata attcgatgag aattgagcaa gcttccgcct tgcgcgaaaa aaatgttttt
    16981 gattatcctg aagtagctat tcgagaaata ctattgaatg ccattatgca tcgagactat
    17041 caatcgaaca cgccagttaa actgtattgg ttttctgatc gaattgaaat tcacagtcca
    17101 ggtgggctat atggtgaagt tacagctgaa acattaatcc gtcgcagcag ttatcggaac
    17161 cctgttttgg ctgaggctat gaaggcctta ggatatgtta atcgatatgg atacggaatc
    17221 cagcgagcga tgtcagcaat ggaaaaaaat ggcaacaatc caattcactt tgaaatggac
    17281 gaccgtactt ttttagctgt cttgccgagg atttctagtt gaaaactgta gcttttttta
    17341 ataataaagg tggtgtaggg aaaacatctc tggtttacca cctggcctgg atgtacgcag
    17401 atcaaggcat acgaacgctt gctatagatt tagaccctca agcaaatctt agtgcaatgt
    17461 ttttggatga agatcgttta tcgcaactat gggaggctgc gagcgatgca actgttatgg
    17521 gtagcttgct gccgcttatt aatcgaacag gagatattgc tgacccacat gtggaaaaaa
    17581 ttacggaaat gctcgggttg gtgtgcggca atttagcact gtctagattc gaggatttat
    17641 tgtcggaaaa ttggcctaaa tgcttagatg gcaaggagga cgcattccga gttattaccg
    17701 catttcaccg cataattgaa cgagcggcgc aactgatgtc agcgcaactt atcttaattg
    17761 acgtcgggcc taatttaggg gcaattaatc gttcggcgtt aattgctgcg gaacaagtag
    17821 tgttgccatt agcacctgat ttattttcga tccagggcat gaaaaactta gggccaacat
    17881 tgcgggattg gcgtaacgga tggaaaaagc gaatgagtga gttaccttca agcgcaaata
    17941 tttccgcgcc atctggaaca atggcccccg ttggttatgt agttatgcag catggtattc
    18001 gtgatagcag gccggtaaag gcctatttac ggtggatgga aaaaattcca acagtcttta
    18061 gggactcgtt actgggcgaa caagaacagt tgttaacggt taagaatgac ccatacaatt
    18121 tagcgttact caaacactac cgcagtttaa tgcccatggc gatggaagca aataaaccta
    18181 tgttttttct taaacctgct gacggtgcta ttggctcaca cgttgaggct gttagattgt
    18241 gctatcaaga tttccatgct ctggcgaatg taattgcgtc tcatgcaggc gttcatatgt
    18301 cgtaatccta aaagattcgg ttaaatcgga tttcgcttgc gctgcatcca ggctactcag
    18361 ctgagaataa aatgcatcaa acagtctttt atcaataatc acatcaccca attcaagcct
    18421 gcacctgcac ctgcaccaca ggccttcgtc cccactgggc ggcaaataag cccgccaaca
    18481 gcataatccc cataattaaa atcgcctgcc agcccagcag ggcataaacc cagccggtta
    18541 gcccagaggc gagggttacg gaagtgaagg tgcaaaattc gttgatggct tgcagccgtg
    18601 cgcgcagggg ggcgtcgggg atttgggtga ggagggtgct gccgcccatg tacataaaat
    18661 tccagcctac acccaccaca aacaaggcgg cgtggaaggc tatgaggctg gtgctggcca
    18721 gggctacggc gcagccagcg gcgagtatgg ctacccccgc ccaagcaacg ctgggcgcgc
    18781 cccagcgggc gatccatttg ccggtcatta gggagggggc gaacatgcct aataggtgcc
    18841 attggattac gctcgcggca tctccggcgt ggaagccgca gccggccatg gccagggggc
    18901 tggctagcat caccatcagc attaggcagt agcccaccgt gcagaacaac atggcttgca
    18961 cggcgattct aggcaggggc caccaggtga cggcggtgtt gctggcggct tggcgcttgg
    19021 gcagcggcgc aaaggcgaag acggggatgg ccagcaggca cagcaccata gcggccaggt
    19081 acgcgccggt gtattccacc ggcaggctgt gtaaaaagtt tttgccaatc atagggccga
    19141 tcagggcggc ggctatgccg ccgcttaata cccaggcgat ggcggtactt tttagggggc
    19201 cgtccacggc gtcggcggcg gcaaagcggt aatacagggc ggaggcctgg tacacgccga
    19261 tcagagcgcc cgcaatacaa aacagggcga attcttcaaa ccaaatactg taggtggcca
    19321 gggcgcagcc gagggcgccc actgcggcgc cgagtataaa cacccccctg taccccaata
    19381 ggctcaggcc cttgggcata aacaatgtga gggcggcggt ggcgcccatc atgcctagga
    19441 atggcagagt ggcgaggctg gggctggggg ccagttgttt gcccaccagg gagttaaagg
    19501 caatgcccac tgccacggag gccataaaca aaaattggca aagcccaagg cagattgcgc
    19561 tgatggacat aaatgtggct cccaaagtgt ggccaaaagg taatgaacag ggacaataac
    19621 ccaacgggcg tgccctgggt atggttcggg tggcagtctt acgccacctg tggcgtttaa
    19681 ctaaaactac agttagaccc cgaatgccga gggtttggat ctcaatggtg ttttcaaaaa
    19741 ctcaccaaga cgggtgctag tgcttcaact gaagtgtttg ggtctaatcg gagaataagc
    19801 cgccgccagg ggctttttgt tctggaagca gatagtcccg atagcggctg ttgatcagca
    19861 cctggtactg atcgtccggt agggcggtga attgaccggt tttcaggtgg tattggtcaa
    19921 agaccagttc atcggggcag tcttcaatgc cccctgtata acacaggcgc actggggctg
    19981 tgtcgctcgc atccactgcc cgtttgaacc agcgggtggc gcggtaatgg ttgccaacta
    20041 cttgggaggc caggctgaag ggcccgctga agcctgcggg gcgccgtgtg ggttggcggg
    20101 cggtaatacg cagccactcg ccgccggttt ttaataggcg ggccagatag ggggtatcga
    20161 acaacaggct gtgtactttg gattccaggg agataatcag gtcaaagctt gcggcggcta
    20221 ggggcaactg gctgaggctt tggctgccag agtagagcag gctgagattg tcaaacccct
    20281 cccgctggca gaactcgctc tgccactggc gccagagctc aagggtggtt tgattcgggg
    20341 tgacggcgaa cacctcgccg gctggcccgg cctggtgggc caaacaaaat acttcctgcc
    20401 cgttgccctc ccccagcagc aaaatgcgca ggccgccgat ggcggtaggt atgccaaaac
    20461 ttaacgcggg cgagatggcg agcttttgca gggggctggc gatttgctgc cagcagccgc
    20521 tggggcagca gcgctggcgg gtttcttctg atgttaagga tggggcatat acgacgtgcg
    20581 cgcgactggc cataatgaag gttccgtaag ctgctagttc cgccctagac tttagggcta
    20641 agccgctcac ttgagtaaag tgatcgtcca gaatttgcat ccaatcgtcc ggcaggaggc
    20701 catttttttg acctcgtagc cggtacaccc ctgcatgctc aagctcagtg aagtgctcaa
    20761 tcgatttaac ctcagtgccc aggtgtttta cgccggtgat gtgtgcggca ccctggcgtt
    20821 tgagcaggcc agccccttag agggccatat gcatttgatt aaacaggggc ggctggatat
    20881 tcgcgccggg gatgcctggg cctatgtgat tgagcgaccc agcctggtgt ttatggccag
    20941 aagccagcag catcagctgc gcgcccaggt agaggcgggt gcccagg
//
LOCUS       DAOQGS010000004        20454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1223_length_20454_cov_4.752194, whole genome shotgun sequence.
ACCESSION   DAOQGS010000004 DAOQGS010000000
VERSION     DAOQGS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 20454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20454
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_20454_cov_4.752194"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2358)
                     /locus_tag="PKZ52_00340"
     CDS             complement(<1..2358)
                     /locus_tag="PKZ52_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58239.1"
                     /translation="MRRFTIKGLSLFTSKALIKLFSHFSIIVLLLLCSLWILRAPLVQ
                     WAARQPLPNDWAVELTGLTSPSITQWRFKELTVKHRGDIVCSLREGLLQWNPALLWQK
                     KLLIKRLNLESATIYQRKTETNGAFSAPDFSQLPSIAVKEASIGHLAFIDNTASPTQL
                     AAQLNREDYAFNGDIHVLWGDTLLAVNAQLHNQQGALIAAINTQAKSEQAWSISAQID
                     EPAGGMLGQQLTLPPAQPLKAKAQLDIEYFDKRYQINLVQLELPWQDQLIKSQGTLWV
                     NAERTSIEVRELLLSTNGQTQRIKALITPDDKQMSAQLEQFPLGLLSPWLPLQGGSAS
                     GDINAYWHSARAELSLNANAEFTTVVNGWPLFVRGEFNYSPEHLLTIQQGQARWGEGA
                     LKTIIKADGSLDFKGDKSNLSVSSDFFTQQHLRNLPIKWLNEPANRLARELHALPFSA
                     KVANLNLQGPLKDPLIRFNLDAKTQWFAHSFDLHLEGKGDMRDAELTNTVLQQGSGSI
                     TLNGRFDWRGASNKMQLSLSNWGEAFWPVYQLTPLITAHARVNGAASLTGSLSNPIIN
                     SQLNVTGEWAFNELTAFTLINNAQYQYNFAKPWPQGLSLLATDQIELKLNDQPRLALS
                     GSYTAKNLDLSLKCANWDAQLNQLLQLPLAPGNGDLQLNLTGSPQAPIINGQWQYSLS
                     APGNEPLHWQGKLATQSNNLIIESDLKEGEIPLAQLRGRYPIKGLWQPQQLDGQLELT
                     AKLAASRLMLDNRRYPTSGDLQVNLALGGTADAPTVDGQINISAGS"
     gene            complement(2360..4108)
                     /locus_tag="PKZ52_00345"
     CDS             complement(2360..4108)
                     /locus_tag="PKZ52_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNG58240.1"
                     /translation="MNSLLFISVLTLFWAMGAQAQLPRWNFWQQQHPFSIEVAGELSG
                     HPDLAKQLTQQLTQERKNNPDILALDDATSIAQQELGVLESLLRTQGYYNGRASLKNL
                     KPLRYQVEPGSQFFIEQLILDLPESDPPLPSLALSQGDPLMADAVFNALEQIKHHYSQ
                     HACYYPLEVKYQVKIDKTTAKAQVRIYLASDTPAQFGASQFHGAPNINANFLQYYLAH
                     QIGDCFKQAPLDKMRLNLLKSDLLTSADIQTRLDGNQVLTDIYLTERKPRTIKGGLSY
                     DGDNKEGLSLGWEHRNLWGNGEHLSLTAEQNRIQGDYAARLTLPHILHPQQNLILSAE
                     YKPEDSPSSQSDTELLAASLERHFSEQFIVSLGVETELIQEKQARRSGLISVPARLYL
                     NNTDALLNPSQGWALTLGASSTWDAYHPEIQFQNQTIAASFYYTYVRKGLVPTLALRF
                     ASGTLGGEALDSIPATKRFYVGGGGSVRGYPFQSLGALEEGKPTGGLSFAESSLELRL
                     RWGEDWGLAAFYDGGWAYPDANWHWGEQFLWGYGLGLRYYTLFAPIRLDVARPKTLRP
                     GIDDPYQIYVSLGQAF"
     gene            4234..4842
                     /locus_tag="PKZ52_00350"
     CDS             4234..4842
                     /locus_tag="PKZ52_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58241.1"
                     /translation="MSADTATSAERNLTGIVRINEKIKQVVSLAFNINLLALNALVLS
                     RQAGDAALGFAVISAELRSFSRELAQAMAALSDTSFELVNLVSLAARKGHKHQLLARA
                     LAAYQGPLNQRLALSSTHLNAEEKALAARFEHLHEHFERAGLSVRLGTIIARSLKIEA
                     TYGGSQEAMLAQVACEFDRYIDTIPRLLAELKHHLPPRALML"
     gene            4839..5585
                     /locus_tag="PKZ52_00355"
     CDS             4839..5585
                     /locus_tag="PKZ52_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNG58242.1"
                     /translation="MKKAQTIYQNGEHFWQVIARDPARPNYLIDTNEYLISYHGQALL
                     TDPGGVEIFPAVFSAIAQAGNPEQICALFASHQDPDIISSLSMWLELNPALPCYVPWL
                     WAGFIPHFGGNQDTFRPIPDEGMAIDLNGLPLTAVPAHFLHSPGNCHLYDARARILFS
                     GDVGAALLPDDYPSVFVEDFDRVIKAAAGFHRRWMGSNSAKLDWCERASKMDIDMLCP
                     QHGAIFRGEDVGRFINWFAELNVGVLALAK"
     gene            complement(5644..6216)
                     /locus_tag="PKZ52_00360"
     CDS             complement(5644..6216)
                     /locus_tag="PKZ52_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNG58243.1"
                     /translation="MMLLKPTGCSLLAALIFASIPAQADWLLEPEINSGSLKLSNNFH
                     FSPGDSHENSLNAYALGVQAGFEFANHVFVKAGLTDGETEHLLGWQDRIKFYDQHIST
                     GVQWPVSKRVSLLGSLGISHWDYYSKDQDSFNKNQPDKHYSGSGVQYRGGVNFRFNSL
                     LSMNLYAGSKDWGDDVRYAQYGVSLCFRFE"
     gene            6420..8579
                     /locus_tag="PKZ52_00365"
     CDS             6420..8579
                     /locus_tag="PKZ52_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HNG58244.1"
                     /translation="MAIGGGFHALHSVLHYAWQIGPFKLWQSLRSKNACKACAFGTGG
                     QNGGLKNEAARSFEVCNKNIQAHTSDIRPGIPAQYFLQTPISAMARLSGKKMEALGRL
                     TLPLYKPAGAKHYSPLSVDEAINLAASRLKAIKPERSFFYASGRSSNEAAFALQLLAR
                     LYGTNHINNCSYYCHQASGVGLKACLGTSTATVTFEDLNLADCIFVWGANPASNHPRF
                     VKLLLQARRRGVKVVVINPAQEAGLLRFASPSDWRSMLAGGSQVSSLYIQPHLGGDSA
                     LMAAMAKLLIEQNALNEGFIANYTEGFDEFKTAISALNWDDLIAQAGVSYEQIKTLVN
                     LYQNSERCIFAWSMGLTHHTQGVGNIEGLAALALLRGQIGKPGAGLLPLRGHSNIQGT
                     GSMGFTPQLQASTLAALEQQLGFNLTQAAGLDSMACMQAAHDGQMDALVGLGGNLWAA
                     NPDTAFTREALNRIGFKCFLTTTLNHSHVEGIDGEVVIFPVRARDEEAAPTSQESMFN
                     FVRLSDGGIERFPQLLSEIDIIARIGQQLINAAQFDFGLLKNPKTLRQWIAKVIPGYQ
                     ALAKVDENKQEFTVGGRILHSPEFATASGKAQFKFSPLPIYEAGYRLTSVRSEGQFNS
                     IIYHEQDAYREQTERWVVMMHPEDMAKEGLKENQTINLTTATGSMQALKVKPYNIRPG
                     NLMVYYPEANQLIPRAVDTRSKTPGFKSVVVRLSLRN"
     gene            8885..9871
                     /locus_tag="PKZ52_00370"
     CDS             8885..9871
                     /locus_tag="PKZ52_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58245.1"
                     /translation="MSKELLIDECKKLCRELRLSRLENLIPTMHNFSDFDGPSPHECE
                     NDIRFVFGVFKNLDENRAWGEISWYALKSLGQRLREISNLASQCHSSRDQAIYESFAL
                     GLDSFVHELRASGISFLSEGGANIERASVALSLEMEKVIKARDNFEKLQDEVRMLITP
                     VVAGSLSQSFAARKRVLFLTRMVWGVISIIVGYFSIYFTIQVANYIAGLFLDSKALES
                     KIELLWPSIFVRSIILLPFYVAFGFSFSQYKKERDFEEEYAHKAAVATSLPNYGDLTR
                     EPAIRDQIVMGATNVIFSSPISKVCESERQDKVLGGIKEVFDSLSKLIPKKD"
     gene            complement(10607..11131)
                     /locus_tag="PKZ52_00375"
     CDS             complement(10607..11131)
                     /locus_tag="PKZ52_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58246.1"
                     /translation="MKHLFIALASLGLAAHTYAADCNLVYDEFDSLMNKQFLITPGIY
                     TSVKENRISRSEYNTLQKGKLLLKAENAGYGVAVVHTNNNTWGKLLYTWSAGGAELII
                     KEATLYGRVLDGFAPRTLRNIRIPSSYTYDLDTGTLGGTGGDIWFNNVDGNLMYLSEY
                     GGADLSFPMRSLCD"
     gene            11423..12415
                     /locus_tag="PKZ52_00380"
     CDS             11423..12415
                     /locus_tag="PKZ52_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNG58247.1"
                     /translation="MAWQAIPTNIITGFLGAGKTTAILHWLKHKPADEYWAVLVNEFG
                     EVGIDGALLKGRGAMIKEIPGGCMCCVAGLPMQVGLNRLIAEAKPARILIEPTGLGHP
                     AQIVAALTSPPFDKLLKLQASLCLIDPRKVQDARYLEHETFQAQAALADVLVASKVDL
                     ASQQDMDAFEAFADNLPGLKHRAHSQQGDLDLAWLTKPYGNKQLPTSAAHASQNNPLA
                     NRFLQLPAPAEGELFIRKQNQGQGFYSCGWVFRPEAVFAMEPLFIWLSGLVCQRVKGV
                     MITTEAIIGFNAEEGVLSMNELDECDDSRLEIIHPSPLDWDAIEQVLLSCLAEA"
     gene            complement(12389..13315)
                     /locus_tag="PKZ52_00385"
     CDS             complement(12389..13315)
                     /locus_tag="PKZ52_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNG58248.1"
                     /translation="MGRFLVGILTLLCLMPPTAAAATSLFVVTRSDQPGHSLLLGGSI
                     HLLRQTDFPLPPEFTAALSQANQLVLESDVSSTQQAQLGARMLSLSQLEAGKTLADLL
                     SPALWQRLSAYCQKANLPLEQLQQSKPFFFSMMLTVTHLQRLGFVPGVDYYLDEQARK
                     SGKPVGQLESAEEILAMMKAVDNLKPDDIIKSTLDDMDRIEPMLAGVLKAWREGDLGV
                     IQKEMVAPMQEQMPLLYKLVLVERNQRWLPQIERLFTSPGVELVVVGSAHLTGPDGLI
                     SQLIARGYRVSAWTAPAPKVIQPASSLRQAAQ"
     gene            complement(13318..13977)
                     /gene="yihA"
                     /locus_tag="PKZ52_00390"
     CDS             complement(13318..13977)
                     /gene="yihA"
                     /locus_tag="PKZ52_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNG58249.1"
                     /translation="MSQTINFRIAQFLISAPTLAQCPPETGVEVAFAGRSNAGKSSAI
                     NTLTNNGKLARTSKTPGRTQLINIFTLGDANKRLVDLPGYGFAKVPLATKLQWQEHLA
                     EYLYERRCLKGLVLLMDIRHPLQEFDQLMLDWANKAGKQVHVLLTKSDKLNRGQTKTT
                     LLQVGQYLKNNHYTQTSVQTFSALNKDGLGELEQTLARWLLDENQETPDGASDSSAET
                     S"
     gene            14259..14915
                     /locus_tag="PKZ52_00395"
     CDS             14259..14915
                     /locus_tag="PKZ52_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNG58250.1"
                     /translation="MKTSIQAWLFILGAVLVAPVFAAGDAKQGETLVVACLACHGDGG
                     NSTTPNFPKLAGQGEKYLLKQLKDIKGGMRVVPEMTGVLDKLGDSDLANIAAFFASKS
                     MQLSGSKELKVKVNSGEQVDGLTLGAKIFRAGNVASGVPACSGCHSPVGLGNAPAGYP
                     RLGGQHAAYIEKQLKNFRLGERANDGEAKVMRQVAQQLSDAEITAVANFIAGLNAGTN
                     "
     gene            15049..15720
                     /locus_tag="PKZ52_00400"
     CDS             15049..15720
                     /locus_tag="PKZ52_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNG58251.1"
                     /translation="MRFAALVVGFLLPLFAFAEAAPAAPKAPAAIEEGKQYEVLEGAA
                     KPVPGAKIEVTEVFWYGCGHCYAFEPILEPWVKKLPADVKFTRSPAIWPTRRPGMPED
                     LMKTHAKLYYTALAMNAVDKLHQPLFDALQKEGKGMYEDAEIAAVVKAAGVDGDKFVA
                     TMKSFAVNSQVTQADARQKAFKVSGTPEIVVANYYKISASAAGGQKEMLDVADFLIKK
                     LQAAK"
     gene            15891..16808
                     /locus_tag="PKZ52_00405"
     CDS             15891..16808
                     /locus_tag="PKZ52_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNG58252.1"
                     /translation="MTALNPVSRTRMAPNGPEVSRLIAGFWRLKHWGMSTQARLSFIE
                     QLLELGITTMDHAMVYGSEGLFGEALALRPSLRSQMQIVTKCGIRPCGFGPLGAQKVN
                     HYDASAAAIVQSVDNSLSALGTDYMDLLLIHRPDYLLQVDEVAACFAALKAAGKVKYF
                     GVSNFTPSQFASLQMACGGSLVTNQIELSPLQMAPLDNGLLDQCVQQKIKPMAWSCLA
                     GGALLAPTEPRGERVLAALNEVAEELGLVDIEPVVYAWVMTLPCDPLPLLGSSKIERI
                     KIAVQADALRLSREQWYRIWEASNGACVP"
     gene            16874..17776
                     /locus_tag="PKZ52_00410"
     CDS             16874..17776
                     /locus_tag="PKZ52_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNG58253.1"
                     /translation="MPNTPTLDAAATPKPRHWYASLNLQLGERAGKTRLLSAAHQGPL
                     RVQRAFYPEGPHCHLYWLHPPGGLVTGDELSLSAQLLPGAQVLLTTPSAGKIYAVEGA
                     EGAAATPGQARPQLTSFSLTQAQDSYLEWLPQETLVFNGANARISTRFNLSGNARLFA
                     WDLVCLGRPASSQWFNTGRCHTSFEVWQDGQPLQIERTDLAAGTRFFNAPWGLKGAHS
                     LGTCLASLVLLRDEQEALLAALTAQFGGGENLWGVTQKGPLLLVRYLGCDMAVAKAGC
                     VFVWQQLRPKLINRPACLPRIWAT"
     gene            17834..20224
                     /locus_tag="PKZ52_00415"
     CDS             17834..20224
                     /locus_tag="PKZ52_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HNG58254.1"
                     /translation="MYRLFLPAGAILFLPAGARLLRAPLLRLWLWLSFVVISGCAQLQ
                     FEQWDAHYGKPIPKEVKTSAAGPLTYYGQIRPLLEKRCVNCHGCYDAPCQLKQEAYEG
                     ILRGANAQPVYSGVRLLAAEPTRLFEDAHTTAQWRDKGFYPVLNERREEGNNNLQLSL
                     LAQTLLLKKAHPLPAKGLLPESFALGLDAKHECPRIETYQAFAKANPLAGMPYGLPAL
                     KPAESKKLLDWVSQGAPMGAPPSLPPAADEWVQKWEAFLNGPSLKQQLVGRYLYEHLF
                     LAHLYFPKVQGAHFRLVRSKTPPGEPIDLISTRRPYDDPGVARVYYRLRLDPSSLLAK
                     TYMPYALSEERFELWHQWFLNPNFSVDRLPNYSEVESANPFITFAQLPVEARYRFLLS
                     EAQFTIMNFIKGPVCRGQVALNVIQDHFWVFFLAPQTQQGPAFADFLARNSAHLYLPV
                     MESNNLLPVANWATYSRLQMNYLEAKAAYVRESIKGEENLGLGLIWDGDGGKNSNAAL
                     TIFRHKDSASVHKGLIGPAPKTAWVIGYPLLERIHYLLVAGFDVYGNLPHQLMSRLYM
                     DFLRIEGEMNFVELLPKKTQQTELAFWYRDAENDLQRYIDAYLNHLPNRPLLNYRSDE
                     PKQELFQALRQHLGQAAASPHQLNPQTQAEHTLAALQIPPQALKWLPQTTLLLVPEEG
                     VFTLVHVSAYSNLSSLFGEERRRRPLEDQLIVTRGIIGAYPNSFLRVNRNDLALLAAQ
                     MASMNAEADYQALKRRFGVRRTDSNFWANSDEIASIFHKQQPSEAAVLDYNRLENR"
BASE COUNT         4554 a         5172 c         5640 g         5088 t
ORIGIN      
        1 gctgccagcg ctgatattaa tttgcccgtc tacggttggg gcatcggctg tgccgcctag
       61 cgctaaattc acctgtaggt caccactggt ggggtagcgg cggttatcta acattaagcg
      121 gctggcggcg agtttggcgg ttaattccag ttggccgtct aattgttgcg gttgccataa
      181 tcccttaatt gggtagcggc cgcgcaattg agctaagggt atttcgcctt cttttaaatc
      241 gctttcaata attaaattat tgctctgggt agcgagtttc ccttgccaat gcagcggttc
      301 attgccaggg gcgctgaggc tgtactgcca ttggccgtta attatgggcg cttgagggct
      361 gccggtgaga ttaagctgca ggtcgccatt gcctggcgcc aggggtaatt gcagtaattg
      421 atttaattgt gcatcccaat tggcgcactt aaggctgagg tcgaggtttt tggcggtgta
      481 actgccgctt aaggcgagcc tgggttgatc gtttaattta agttcaattt ggtcggtggc
      541 taacaggctt aaaccctggg gccagggttt agcaaaatta tattggtatt gggcgttatt
      601 aattaatgta aacgcggtga gctcattaaa ggcccattcg ccggttacat ttaattggct
      661 attaataata ggattgctga ggctgccggt aaggctagct gcgccattca cccgcgcgtg
      721 ggctgttatt aagggtgtta attgataaac cggccaaaag gcctcgcccc aattgctaag
      781 gctcagctgc attttattgc tggcgccgcg ccaatcgaag cggccattta gagtgatgct
      841 gccgctgccc tgttgcagca ccgtattggt taactcggcg tcgcgcatat cgcctttgcc
      901 ctctaaatgc aggtcaaaac tgtgagcaaa ccattgggtt ttggcatcca ggttaaacct
      961 gataagcggg tcttttaacg ggccttgcag gtttaaattt gccactttgg cgctaaaggg
     1021 cagcgcgtgt aattcccgcg ctaagcgatt ggccggttca ttcagccatt taatgggcaa
     1081 gttgcgcaga tgttgctggg taaaaaaatc gctggaaaca cttaagttac ttttatcgcc
     1141 tttaaaatcc aaactgccgt cggctttaat aatggtttta agagcgcctt ccccccagcg
     1201 ggcttgcccc tgttgaatag tcaataagtg ctcaggagaa taattaaatt caccgcgcac
     1261 aaacagcggc cagccgttaa cgacggttgt gaattcagcg ttggcgttaa ggcttaattc
     1321 ggcgcgggca ctgtgccagt aagcgttgat atcaccactg gcactgccgc cctgcaaggg
     1381 caaccagggc gacagcagcc ctagggggaa ttgctccagc tgggcgctca tttgtttatc
     1441 gtcgggggtg ataagcgctt ttatacgctg ggtttggccg ttggtgctaa ggagtaattc
     1501 gcgcacttca atactggtgc gctcggcatt tacccacagg gtgccttggc ttttaatcag
     1561 ttggtcttgc cagggtaatt ccaattgcac caggttaatt tgatagcgtt tatcaaaata
     1621 ttcaatatcc aattgtgcct tggcttttaa gggctgcgca ggtggcaatg ttaattgttg
     1681 gccgagcatg ccgcctgcag gctcgtctat ttgtgcgcta atactccagg cctgctcact
     1741 tttagcctgg gtattaatag cggcaatcag cgcaccctgt tggttgtgca gttgggcgtt
     1801 gacggctaag agtgtatcgc cccataacac atgaatatcg ccattaaagg cataatcttc
     1861 gcggtttaat tgtgccgcaa gctgggtggg tgaggcggtg ttgtcgataa aggccaggtg
     1921 cccaatagaa gcttctttta cagcgatgct gggcaactgg ctaaaatccg gcgcgctaaa
     1981 agcgccgttg gtttctgttt tgcgttgata aatcgtggcg gactctagat ttaagcgttt
     2041 tattaataat tttttttgcc acagcagggc cgggttccac tgcagcaatc cttcgcgcag
     2101 ggagcaaaca atgtcgcccc tgtgtttaac ggttaattct ttaaaccgcc actgggtaat
     2161 actcgggctg gtaaggccag ttaattccac ggcccaatcg ttgggcagcg gttggcgagc
     2221 ggcccattgc actaaaggcg cgcgcaaaat ccacaggcta cataacagca acagcacaat
     2281 aatgctgaag tggctaaata atttaatcag cgctttgctg gtaaataggc tgaggccttt
     2341 gatcgtaaag cggcgcatat taaaacgcct gccctaggct gacataaatt tggtaggggt
     2401 catcaatgcc cgggcgcagt gttttgggcc gcgccacatc cagccgaatg ggcgcaaata
     2461 aggtgtaata acgcaggccc aggccataac cccataaaaa ctgttcgccc cagtgccagt
     2521 tggcgtccgg gtaggcccag ccgccatcgt aaaaagccgc cagaccccag tcctcgcccc
     2581 agcgcaggcg cagctccagg gagctttcgg caaaggagag gccgccggtg ggtttgccct
     2641 cctccagcgc gcctaggctt tggaagggat aaccgcgcac cgagccgccg ccgcccacat
     2701 aaaagcgttt ggtggcgggg atggagtcca gggcttcgcc cccaagcgtg ccgctggcaa
     2761 aacgcagggc gagggtaggc accaagcctt tgcgcacata ggtgtaataa aaactcgccg
     2821 caatggtttg attttgaaat tgaatttccg ggtggtaggc atcccaggtg ctgctggcgc
     2881 cgagggtgag cgcccaacct tggctgggat ttaacagtgc gtcggtatta tttaaataca
     2941 accgcgcagg tacagaaatt aacccggagc ggcgcgcttg tttctcttga attaattcgg
     3001 tttccacacc gaggctgaca ataaattgtt cggaaaaatg ccgctctaaa ctcgccgcta
     3061 ggagttccgt atccgattgg ctggaggggg agtcctccgg tttatattcc gcgctgagaa
     3121 ttaaattctg ctgcgggtgc agaatatgcg gcagggttaa gcgggcggca taatcccctt
     3181 ggatgcggtt ttgctctgcg gtaaggctta aatgctcgcc attgccccat aaattgcggt
     3241 gctcccagcc cagggaaagg ccctctttat tgtcgccgtc gtaacttaaa ccgcctttaa
     3301 tggtgcgcgg tttgcgctcg gtgaggtaaa tatcggttaa tacctggttg ccgtctaagc
     3361 gggtttgaat gtcggcgctg gttaataaat cgctttttaa taaattcagc cgcatcttat
     3421 ccagcggtgc ctgtttaaaa cagtcgccaa tttggtgggc taaataatac tgcaaaaaat
     3481 ttgcgttgat attcggtgcg ccgtgaaatt ggctggcgcc aaattgcgcg ggggtatccg
     3541 aggctaaata aatccgcacc tgggctttgg cggtggtttt gtcaattttt acctggtatt
     3601 taacctctag cgggtaataa caggcgtgtt ggctgtagtg atgtttaatt tgctccaatg
     3661 cattaaatac cgcatcggcc attaaagggt ccccctggga gagggccagg gagggtaggg
     3721 gcgggtcgct ttccggcagg tctaaaatta attgctcgat aaaaaattgg ctgccgggct
     3781 ccacctggta gcgcagcggc ttaaggtttt taaggcttgc gcgaccgttg taataaccct
     3841 gggtgcgcaa gaggctctcc agcacgccta attcctgttg ggcaatactg gttgcatcgt
     3901 ctagggccaa aatatccggg ttatttttgc gctcttgggt gagctgttgg gtgagttgct
     3961 tggcaaggtc tgggtgtccg ctaagctcgc ctgccacctc aatactaaag gggtgctgtt
     4021 gctgccaaaa attccagcgc ggcaactgcg cctgcgcgcc catggcccaa aacagcgtca
     4081 gtaccgagat aaaaagtaag gagttcaacg tgttaaccgt aggtgggaca caaggggcca
     4141 gtgtaccgcg tttacttgcg cgcttgcagg cttgaggatt tagcgccggt ttgctgctat
     4201 accctaggtg gatgcattga cgagggggca gccatgagcg cagatacagc cacgagcgcc
     4261 gagcgcaatt taaccggcat agtgcggatt aacgaaaaaa taaaacaggt ggtaagcctg
     4321 gcctttaata ttaatttgct ggccttaaac gccctggtgc tctcccgcca ggcgggggat
     4381 gccgccctgg ggtttgcggt gatttccgcc gagttgcgca gctttagccg ggagttagcc
     4441 caggccatgg cggcacttag tgatacctcc tttgagctgg tgaacctggt tagcctggcc
     4501 gcccgcaaag gccataagca ccagttgttg gcccgcgccc tggcggccta ccaaggccca
     4561 ctcaatcagc gcctggcctt atcaagtacg cacctaaatg cagaggaaaa ggccctggcg
     4621 gcgcggtttg agcatttgca cgagcatttt gagcgggccg gcctgtccgt gcgcctgggc
     4681 accatcatcg cccgctcgtt aaaaatagaa gccacctacg gcggcagcca ggaggctatg
     4741 ttggcccagg tggcctgtga atttgaccgt tacatcgaca ccatccctcg attattagcc
     4801 gagcttaaac accacttacc cccgcgagca ttgatgctat gaaaaaggcc caaaccattt
     4861 accaaaacgg cgagcacttt tggcaggtaa tcgcccggga tccggcccgg ccgaattatt
     4921 tgatcgacac caatgaatat ttaattagtt atcacggcca agccttattg accgacccgg
     4981 gtggggtgga gatatttccg gcggtgtttt cggctattgc ccaggcgggt aacccggagc
     5041 agatttgcgc gctgttcgcc tcccatcaag accccgatat tatttcgtcc ctgtccatgt
     5101 ggttggaatt gaaccccgct ttaccctgtt atgtcccctg gctgtgggcg ggttttatcc
     5161 cacattttgg cggcaatcag gacacctttc ggcccatccc cgacgagggt atggccattg
     5221 atttaaacgg cctgccctta accgctgtgc ccgcgcattt tttacattcg cccggtaatt
     5281 gccatttata cgatgcccgc gcgcgtattt tatttagtgg cgatgtaggg gcggctttat
     5341 tgccggatga ttaccccagc gtatttgtgg aggattttga ccgcgtaatt aaagcggctg
     5401 ctgggtttca ccgccgctgg atggggtcta attcggctaa attagactgg tgtgagcggg
     5461 cttctaaaat ggatatcgat atgctctgcc cgcaacacgg ggcgattttt cgcggtgagg
     5521 atgtggggcg gtttattaat tggtttgcgg aattaaacgt aggggttttg gctttggcta
     5581 aatagcctct tttgggtgaa tcaaaccccc aatgtattaa gccataaata actatgctgt
     5641 tttttattca aagcgaaaac acaggctaac cccatattgg gcgtagcgca catcatcgcc
     5701 ccaatcttta ctgcccgcgt atagattcat ggaaagcaga gaattaaaac ggaaattaac
     5761 tcccccacgg tattgcacac cgctgccgga atagtgttta tccggctgat ttttgttaaa
     5821 gctgtcttga tctttgctgt aataatccca gtgggaaata cccaatgaac ccagcaggct
     5881 gacccgtttg ctcaccggcc actgcacccc ggtggagata tgttgatcgt aaaatttaat
     5941 gcggtcttgc cagcctaata aatgttcggt ctcgccatcg gttaaaccgg ctttaacaaa
     6001 tacatgattg gcaaattcaa accccgcctg cacgcctagt gcgtaggcgt ttaggctgtt
     6061 ttcgtggctg tcgccggggc taaagtggaa gttgttgctt aattttaacg agccgctatt
     6121 aatctccggc tctaatagcc aatcggcctg ggcgggaata ctggcaaaaa taagggctgc
     6181 cagcaggcta catccggtag gtttaaggag catcatgggg aattccttcg tgtgggtggg
     6241 caggggaatg tggcagcgca taactgaatt ggggctgaat tttgtggtgc ccctgggcct
     6301 ttgtaactgg tttgtctatg gggtaaataa cggtctgtgc gcggggagga tttgcgccat
     6361 aatgcggcca ttgagcacgg caattaacac ttctttttca acacatttga ggcgccccta
     6421 tggcaattgg tggtggattt cacgccctgc acagcgtttt gcattacgcc tggcaaattg
     6481 gcccatttaa actctggcag agcctgcgct ctaaaaatgc ctgtaaagcc tgcgcctttg
     6541 gcaccggcgg gcaaaatggc ggcttaaaaa acgaagcggc gcgcagtttt gaagtgtgca
     6601 ataaaaatat ccaagcccac accagcgata ttcgcccggg catacccgcc cagtattttt
     6661 tgcaaacgcc catcagcgct atggcgcgct taagcggaaa aaaaatggaa gcgttaggcc
     6721 gcctaaccct gccgttatac aaacccgccg gggccaaaca ctattcgccc ctaagcgttg
     6781 atgaggcgat taacctcgct gccagccgcc taaaagccat aaaacctgaa cgcagttttt
     6841 tttacgcctc tgggcgctct tctaatgaag cggcttttgc cctgcaattg ctagcccgcc
     6901 tgtacggcac caaccacatt aataattgct cctattattg ccaccaggcc tcaggagtgg
     6961 gccttaaagc ctgtttgggc acctccaccg ccacggtgac ttttgaagat ttaaaccttg
     7021 cggactgcat ttttgtttgg ggcgccaacc ccgcctccaa ccatccgcgc tttgtaaaat
     7081 tgttgctgca agcgcgccgc cggggtgtga aggtggtggt gattaacccc gcccaagagg
     7141 ccggcctgct gcgctttgcc tcccccagcg attggcgctc catgttggca ggcggctcgc
     7201 aggtttcaag cctgtatatt cagccccatt taggtgggga tagcgccctg atggcggcca
     7261 tggcgaaatt attaattgag caaaatgccc ttaatgaagg ttttattgcc aattacaccg
     7321 aaggctttga cgaatttaaa actgctatta gcgcgttaaa ttgggatgat ttaatcgccc
     7381 aggctggggt gagctatgag caaattaaaa ccctggttaa cctgtatcaa aacagcgagc
     7441 gctgtatctt tgcctggagc atggggctta cccatcacac ccaaggcgtg ggcaatattg
     7501 aagggttggc ggctttggcc ctgctgcgcg ggcagatcgg caaacccggc gcgggcctgt
     7561 tgcccctgcg cggccacagc aatatccagg gtacaggctc catggggttc accccgcagc
     7621 tgcaggcctc aacccttgcc gccctcgagc agcagttggg ttttaatctc acccaagcgg
     7681 caggccttga tagtatggcc tgtatgcagg cggcccatga cggccagatg gatgccctgg
     7741 tgggcttggg cggtaattta tgggccgcca acccggatac cgcctttacc cgcgaggccc
     7801 tgaaccgcat agggtttaaa tgttttctta ccaccacgtt aaatcacagc catgtagagg
     7861 gtatcgatgg cgaggtagtg atattccccg tgcgggccag ggatgaagaa gccgccccca
     7921 ccagccagga atccatgttt aattttgtgc gcctaagtga cggcggcatc gagcgcttcc
     7981 cgcaattatt aagtgaaatc gacattattg cccgcatcgg ccagcaatta attaatgccg
     8041 cacaatttga ttttggcctg cttaaaaacc ctaaaaccct gcgccagtgg attgccaagg
     8101 tgattcccgg ctatcaagcc ctcgccaagg tggatgagaa taaacaggaa ttcaccgtgg
     8161 gcgggcgcat tttgcacagc ccagaatttg ctactgccag cggcaaagcc caatttaaat
     8221 tcagccctct gcctatttat gaggcgggct accggctcac cagtgtgcgc tcggaagggc
     8281 aatttaattc cattatttac cacgagcagg acgcctaccg ggaacaaacc gagcgctggg
     8341 tggtgatgat gcaccctgag gatatggcca aagagggcct gaaagaaaat caaaccatta
     8401 atttaactac cgccaccggc agcatgcagg ctttaaaagt aaaaccctat aatattcgcc
     8461 ccggtaattt aatggtgtat tacccagagg ccaaccaatt aatccctcgc gcggtggata
     8521 cccgctcgaa aaccccgggg tttaaaagtg tggtggtgcg cttaagtttg agaaattaag
     8581 ttatctaaaa atgaatatat tttttaagct atgctgcggc cgcatggtat aagtgatctg
     8641 ctctcattta gcagcttgtt gaaatgcgta gcctgcgaca ctctcgcagg gcaaatttgg
     8701 cgaaaaggcc ggaatttact cgcagtgaat gagagttttt tcgccaaata taacgccagc
     8761 cagtgctggc gaggtagtat ttcaaaaagc tgttagactg tattttgggt gcattctttt
     8821 ggggttgtgt tgcttgtgtt tgaatttttc ctattgaaaa agataaatta aataatggtg
     8881 ctacatgtct aaagaattat tgattgatga gtgtaaaaag ttgtgccgcg aattgaggtt
     8941 gtcgcggttg gaaaacttaa ttccaacgat gcataatttt agcgacttcg atgggcctag
     9001 tccgcatgag tgtgagaatg atattcggtt tgtatttggc gtctttaaaa atcttgacga
     9061 gaatagagcg tggggtgaaa tatcttggta tgcacttaaa tcgcttgggc aaaggctccg
     9121 agagatttcc aatttggcaa gccagtgtca ctcctcgagg gatcaagcaa tttacgaaag
     9181 cttcgcactg ggtttggatt cttttgttca tgagcttaga gcttccggca tctcattttt
     9241 gtctgaggga ggagcaaata ttgagagggc ttccgtcgcg ctgtcattgg aaatggaaaa
     9301 ggtgatcaag gcgagagata attttgaaaa gctccaagac gaggtgagaa tgcttattac
     9361 tcctgtcgta gcaggttccc tatcccagtc cttcgcagca agaaagaggg tgcttttttt
     9421 gacaagaatg gtttgggggg tgatatcgat tattgtcggc tattttagta tttactttac
     9481 tattcaggtc gcaaattata tagctggttt gtttttggac tctaaggcgc tagagtcaaa
     9541 aatcgaattg ctgtggccgt caatttttgt tcgatccata attttgctgc cgttttatgt
     9601 ggcgtttgga ttttcttttt cccaatataa aaaagagagg gattttgagg aagagtatgc
     9661 gcacaaggca gcagtggcga cttcgctgcc aaattacgga gatctcacca gggagcccgc
     9721 gataagggat caaattgtta tgggggcgac gaatgtaatt ttttcttcgc ccatatcaaa
     9781 ggtgtgcgaa agtgaaagac aagacaaggt gttaggtggc ataaaagaag tattcgactc
     9841 cttatcaaag cttatcccta agaaggatta agatggggct gttgtgttat cgggggtgcg
     9901 aaaatgccgt gctttaattt gattgaggtg tgttgcttat tcggctgctc tttgagtgag
     9961 ttttctgcaa taataagatg atgtttctaa aaataaatgc ttatttggaa aatattgatt
    10021 tttacttttt tggttagggt tgcgcttgcc gtgctggtct tcgatgctaa aaaagtagta
    10081 actattcagc acatttttac ccgctcaatg caggcggcaa attgaggtgg atttatctgt
    10141 gcggcacaat cgatttgcgg tgtagcagtg gtgaatacag gttattgcag accgtcaaaa
    10201 agagggacct ttttgacgag cctacacgga tgtattcacg gcatgtctgc aacaaactgt
    10261 atttatcact gcggcggctt gcagcacgca acactctctt gatgattgtt tgtagatgca
    10321 agaacactcc agccagattc aataagcact gatcaataag tgctgaatag ttacaaaaaa
    10381 taatgcttta tcaatttttg aaatgcccat ttgagaatgc ttccttcgaa agcgaaagcg
    10441 aaggcgaagg cgaaagtgat tgatgctggc cagagtctgc gtgcgaacaa taaagccagc
    10501 gccgtgcagt gagtacaacg ctggtttatt ttagataacc catggggctt atgccgcgcg
    10561 gttagctact tgtaggcggg aggaacccat aggaatttaa taataattaa tcacacaatg
    10621 aacgcatagg gaagcttaaa tccgcaccgc cgtattcgga taaatacatc aagttgccat
    10681 ccacattgtt aaaccagata tcgccacctg tgccgccgag tgtgccggta tccaaatcat
    10741 aggtatacga cgagggtatg cgaatgttgc gcagggtgcg tggggcgaag ccgtcgagta
    10801 cgcggccata cagcgttgcc tctttaataa ttaattctgc accgcctgca ctccaggtat
    10861 ataaaagttt gccccaggta ttgttgttgg tgtgtaccac cgcaactccg tatccagcat
    10921 tctccgcttt taataacagc ttgccttttt gcagcgtgtt gtattcgctg cgggaaatac
    10981 ggttttcttt tacgctggtg taaatgccgg gggtgattaa aaattgtttg ttcattaagc
    11041 tatcaaactc atcgtaaact aaattgcagt cggcagcata ggtgtgggcg gcaaggccta
    11101 aggatgctaa ggcaatgaat agatgtttca tacggtgctc ctggtgattg tgttttggag
    11161 ccagaataat gcactagctg ccagtgtcat ctctgtgtga ttttacccat aagtctgtga
    11221 ccaatttttc cagtgtgttg gtggggttaa ttaaaaagta attttttaat tagtcatcgg
    11281 tttttaattc tttttcattg ccgctaagcc tttaggattt ctcgccccct ggtgccaaat
    11341 tcaattttat tcgccagata tttcataagg ggggtaagtc tgttagcttg cgtccttccc
    11401 tattcacttg gcgcactaat ctatggcctg gcaagcaatc cccaccaata tcatcaccgg
    11461 ctttttaggc gcaggtaaaa ccacggctat tttgcattgg ttaaaacaca agcctgcgga
    11521 tgaatactgg gcggtattgg tcaatgagtt tggcgaggtg ggtattgatg gcgccctgct
    11581 gaaagggcgt ggggcgatga ttaaagaaat tcccggcggc tgtatgtgtt gtgtggcggg
    11641 gttgcctatg caggtggggc taaaccgctt aatcgccgag gctaagcctg cgcgaatttt
    11701 gattgagccc accggccttg ggcacccagc gcaaattgtt gcagctctta ccagtccacc
    11761 atttgataag ttgcttaaat tgcaggccag cctgtgtttg atcgacccgc gcaaagtgca
    11821 ggatgcgcgc tatttggagc acgaaacctt tcaggcccag gccgccctgg cggatgtgtt
    11881 ggtggccagt aaagtcgatt tggcctccca gcaggacatg gatgcttttg aggcctttgc
    11941 cgataatctg cctggattaa aacaccgcgc ccacagccaa cagggcgact tagacctcgc
    12001 ctggttaact aagccctacg gcaacaagca gctgccgaca agcgcagccc acgcaagcca
    12061 aaataatccc ctggccaatc gttttttaca gttgccagcg ccggctgagg gggagttgtt
    12121 tatccgcaag cagaaccaag ggcagggatt ttattcctgc ggttgggtgt ttcggccgga
    12181 ggcggtgttt gctatggagc cgttgtttat ctggttgtca ggcttggtgt gtcagcgggt
    12241 gaagggggta atgattacca ccgaggcgat cattggtttt aacgctgagg agggggtttt
    12301 gtccatgaat gagttggacg aatgcgacga tagccgcctg gaaatcatcc accccagccc
    12361 cttggattgg gatgccattg agcaggtgtt actgagctgc ttggcggagg cttgaggcgg
    12421 gctgaatgac cttgggcgcg ggcgccgtcc aggcggagac cctataaccc cgcgcgatca
    12481 gttggctaat caggccgtcc ggcccagtca aatgggcgct gcccaccacc accagttcaa
    12541 cgcctgggct tgtgaacagg cgttcaatct gcggcagcca gcgttggttg cgctccacca
    12601 gtaccagttt gtacagcagc ggcatttgct cctgcatggg cgccaccatt tccttttgga
    12661 ttacccctaa atctccctcc cgccaggctt tgagcacgcc tgcaagcatg ggctcaatgc
    12721 ggtccatatc gtccagggtg cttttgatga tgtcgtcggg ctttaggtta tccacggctt
    12781 tcatcatggc gaggatttcc tcggcggatt ccaattggcc cacgggttta cccgatttgc
    12841 gggcctgttc gtccaagtaa taatccaccc ccggcacaaa acccaggcgc tgcagatggg
    12901 ttacggtcag catcatggag aagaaaaacg gtttgctctg ctgcaattgt tccaggggca
    12961 ggttggcctt ctggcagtag gcgctcaagc gctgccaaag ggcggggctt aataggtccg
    13021 ccagggtttt acctgcttcg agttgcgaca gactaagcat acgggcccct aattgagcct
    13081 gttgcgtcga cgagacgtcg ctttcgagta ccagttggtt ggcctggctg agggcggcgg
    13141 taaattccgg gggcagcggg aagtctgttt gccgcagcag gtgaatggag ccgcccagca
    13201 acaggctgtg gccgggctga tcgctgcggg tgaccacaaa gaggctggtg gccgccgctg
    13261 cggtgggcgg cattaaacac agaagggtca gtatccctac taaaaatcgg cccatggtta
    13321 gctggtctcg gcagatgagt cactggcgcc gtcgggggtt tcttgattct catcaagcag
    13381 ccaacgggcc aaagtttgtt ctaattcccc caggccgtct ttgttcagcg cggagaaggt
    13441 ttgcacactg gtttgagtat agtggttgtt tttgaggtat tggcccacct gcagcagagt
    13501 ggttttggtt tggccgcggt tgagcttgtc gcttttggtg agcagcacat gcacctgctt
    13561 gcccgccttg ttggcccagt ccagcatcag ttggtcgaac tcctgcagcg ggtggcggat
    13621 gtccatcagc aacactaagc cttttaaaca gcgccgttcg tacaagtatt cagccaggtg
    13681 ctcctgccac tgcagcttgg tggccagggg cactttggca aagccgtaac cgggtaaatc
    13741 caccaggcgt ttgttggcgt cccctagggt gaatatgttg atcagctggg tgcggccggg
    13801 ggttttgctg gtgcgggcga gtttgccgtt gttggttagg gtattgatgg cgctggattt
    13861 acccgcgttg gagcgcccgg caaaggccac ttccacaccg gtctccggcg gacactgggc
    13921 gagggtgggg gcgctgatta aaaactgcgc gatacgaaaa ttgatagttt gggacatgga
    13981 tgatcctagg tgtacggaag gcccctgcgg ggcgagcgcg gcttgagttg agggggtgtg
    14041 aaatttcgcc actcagggtg atgcgcaggg tgagttatag aaacggtgtg tatataatgc
    14101 cagccccttg ggctccgggc tatgcaaagc atccggcgcc aggccttccg gcatgaattt
    14161 acggccccag tggcgcagcg ctgctaggct attacccaac tctggtgtgt gttgccaccg
    14221 ccaggtgatt attgtgacct aagataaacg gaaaccccat gaaaacatcg attcaagcat
    14281 ggttgttcat tctcggcgct gtgctagttg cacctgtctt cgccgctggc gatgccaaac
    14341 agggcgagac gttggttgtt gcctgtctcg cctgccacgg tgatggcggc aactccacta
    14401 cgcctaactt tcctaaattg gctgggcaag gggaaaaata cctgctgaaa cagctgaaag
    14461 acatcaaggg cggcatgcgt gtagtgcctg aaatgactgg cgttttggac aaattaggcg
    14521 atagcgacct ggctaatatt gccgccttct ttgcttcaaa atccatgcag ctgagcggct
    14581 ccaaggaatt aaaagtgaag gtgaattccg gcgagcaagt agatggtttg accctgggtg
    14641 caaaaatttt ccgtgcgggc aacgttgcct ccggtgtgcc cgcctgtagc ggttgccact
    14701 caccggtggg cctgggcaat gcgccagctg gctacccgcg cctaggtggc cagcacgctg
    14761 cctacattga gaaacagttg aaaaacttcc gccttggtga gcgcgccaac gatggtgaag
    14821 ccaaggtgat gcgccaagtg gcccagcagt tgagcgacgc agaaattact gcggtggcta
    14881 attttattgc cgggttaaac gctggcacta attaacccgc ccgctcgcgg cggccccggc
    14941 aaagcgcgtg gcatcacagt tttgccacct tgtgctttaa gccggggaac ttgctccata
    15001 gtatggccgt ctatccgccc gcctaaccct atcatttgag gaaacccaat gcgttttgca
    15061 gctttagttg tcggtttttt gttgcccctg tttgcctttg ccgaggcggc tcccgccgca
    15121 cctaaggccc ctgcggccat tgaagagggc aaacaatatg aggtgcttga aggcgccgcc
    15181 aaaccagtgc ccggcgcaaa aattgaagtg actgaggtgt tttggtacgg ctgtggccac
    15241 tgttacgcct ttgaacctat ccttgagccc tgggtgaaaa aactgcccgc cgatgtgaaa
    15301 ttcacccgct ctcccgccat ctggcccacc cgccgccccg gcatgccaga agacctaatg
    15361 aaaacccacg ctaagttgta ttacacggcg ctcgctatga atgccgtgga taaactgcac
    15421 cagcccctgt tcgacgcctt gcaaaaagaa ggtaagggca tgtacgagga cgcggaaatt
    15481 gccgcagtgg taaaagcggc cggggtggat ggcgataagt ttgtggcgac catgaagtcc
    15541 ttcgcggtca acagccaggt tacacaagcc gatgcgcgcc aaaaggcctt taaagtatcc
    15601 ggcacaccgg agatcgtggt ggccaactac tacaaaatct ccgccagtgc cgcaggtggc
    15661 cagaaggaaa tgttggatgt ggctgatttt ctgatcaaaa aattgcaggc ggcgaaataa
    15721 ctcgccgcca ctttgaaaaa ccgccgctgg gcggtttttt tatgcctgcg atttccatcg
    15781 gcagccggtt ggtgcctcca gggattgcgg cgcgtctaat ccgatttaat ttaaccgcgc
    15841 gcattggctg gttaagatgc cgacccctcg cttaattttt ggaatgctct atgactgccc
    15901 tcaatcctgt ctcccgcacc cgaatggccc ccaatggccc tgaagtctcc cgtttgattg
    15961 caggtttctg gcgtttaaaa cactggggta tgtccaccca ggcgcgtttg agttttatcg
    16021 agcagctctt ggagctgggc attactacta tggatcacgc catggtgtac ggctccgaag
    16081 gcctgtttgg agaagcccta gccctgcgcc ccagcctgcg atcgcagatg cagattgtca
    16141 ccaagtgcgg cattcgcccc tgcggcttcg gccctttggg cgcccagaag gtcaatcatt
    16201 acgacgcgtc cgccgccgcc atagtgcagt cggtggacaa ttcccttagc gccttgggca
    16261 cggattatat ggacctgctg ctcattcacc ggccggatta tctgctgcag gtggatgagg
    16321 tggcggcttg tttcgccgca ctaaaggctg caggcaaagt gaagtatttc ggcgtctcca
    16381 actttacccc cagccagttt gccagcctgc agatggcctg tggcggcagc ctggtgacca
    16441 atcaaattga actctccccg ctgcagatgg cgcccctgga taatggcctg ttggatcaat
    16501 gtgtgcagca gaaaatcaaa cccatggcct ggtcctgttt ggccggcggc gctttgcttg
    16561 cccccaccga gccgcgcggg gagcgggtgc tggcggcgct aaacgaagtg gccgaggaat
    16621 tgggcttggt ggatattgag ccggtggtgt acgcctgggt tatgacgctg ccctgcgacc
    16681 ccttgccctt gttgggcagt agtaaaattg aacgcattaa aattgccgtg caggcggatg
    16741 ctctgcgctt aagccgggag cagtggtacc gcatttggga ggcttccaac ggcgcttgtg
    16801 tgccttgagc cctagcacct gcgcggccta aattattccg tcatcaccat taaaaggccg
    16861 ctttaagttc gccatgccca acacgcctac actcgatgct gccgcaaccc ccaagccccg
    16921 ccactggtac gctagcctca acctgcaact gggggagcgg gcgggcaaaa cccgcttatt
    16981 aagtgccgct caccaaggtc cattgcgggt acagcgggcc ttttacccgg aagggccgca
    17041 ctgccacttg tattggttgc acccgccggg ggggctggtg acgggggatg aattaagcct
    17101 ctcggcgcaa ctactgcccg gcgcccaggt gctgctcacc accccatcgg ccgggaaaat
    17161 atatgccgta gagggagccg aaggggcggc ggctacacct ggccaggccc gcccgcagct
    17221 caccagcttc agcctaaccc aggcgcaaga tagctatctt gagtggctac cacaggaaac
    17281 cctagtgttt aacggcgcca atgcgcgcat aagcacccgc tttaacctca gcggcaacgc
    17341 ccgtttattc gcctgggacc tagtgtgttt ggggcgcccg gcctccagcc aatggtttaa
    17401 tactggccgc tgccacacca gctttgaagt ttggcaggac ggccaacccc tgcagattga
    17461 gcgcacggat ttagcggcag gcacgcggtt ttttaacgcc ccctggggcc taaaaggcgc
    17521 ccatagcctg ggcacctgtt tggcaagcct ggtgttgctg cgggatgagc aggaggcttt
    17581 gcttgccgcg ctgacggcgc aatttggtgg cggtgagaat ctctggggcg tgacgcaaaa
    17641 agggccgcta ttattggtgc gctatttagg ctgcgatatg gcggttgcta aagccggttg
    17701 tgtttttgtt tggcaacaat tgcgccccaa actcattaat aggcccgcct gtttgccgcg
    17761 tatatgggct acctaattta gattgcgacc tgctaacggc cgcccccact gttccggctg
    17821 ttgagacctg ctcatgtacc gactatttct ccctgctggg gccattctgt ttctccccgc
    17881 aggggcccgt ctgttgcgcg cgcccttgtt gcggctgtgg ctgtggttga gctttgtcgt
    17941 catcagcggc tgtgcccagt tgcagtttga gcagtgggac gcccactacg gcaagcccat
    18001 tcctaaagag gtcaaaacca gcgcggccgg gcccttgacc tactacgggc agatcaggcc
    18061 gctgctggaa aaacgctgcg tcaattgcca cggctgttac gacgccccct gccaactcaa
    18121 gcaggaggcc tatgagggca ttctgcgcgg cgccaacgct cagccggtgt acagcggcgt
    18181 gcgcctgttg gcggcggaac ccacccgcct gtttgaggat gctcacacca ccgcccagtg
    18241 gcgggataag ggtttttacc cggtgcttaa cgagcggcgg gaggagggca ataacaacct
    18301 gcagctgagc ctactggcgc aaaccctact gcttaaaaaa gcccacccac tgcctgccaa
    18361 aggcctcttg ccagagagct ttgccctggg cctggatgcc aaacacgagt gcccgcgcat
    18421 tgaaacctac caggcctttg ccaaggccaa cccgctagcg ggcatgccct atggcctacc
    18481 cgcgcttaag cctgcggaga gcaaaaaatt gctggattgg gttagccaag gggctcctat
    18541 gggggcgcct cccagcctgc cgcctgcggc agatgaatgg gtgcaaaaat gggaggcgtt
    18601 tttaaacggc cccagcctca agcagcaatt ggtggggcgc tatttgtacg agcatttatt
    18661 tctcgcccat ttgtatttcc ccaaggtgca aggggcacat tttcgcctgg tgcgctccaa
    18721 aactccgccc ggagagccca tcgacctgat cagcacccgc cgcccctatg acgatcccgg
    18781 tgtggcgcgg gtttattacc gcctgcggct agaccccagc agcctgttgg ctaaaaccta
    18841 tatgccctac gccctgagcg aagagcgttt tgagctttgg caccaatggt ttttaaatcc
    18901 aaatttcagc gtggatcgcc tgcccaacta cagcgaggtg gaatcggcca atccatttat
    18961 caccttcgcg caattgccgg tggaagcgcg ctaccgattt ttgctcagtg aagctcagtt
    19021 caccattatg aattttatta aggggccggt gtgtcgcggc caggtggcgc tgaatgtgat
    19081 tcaagatcac ttctgggtat tttttctcgc cccgcaaacc cagcaagggc ccgcctttgc
    19141 ggatttttta gcgcgcaaca gcgcccattt atatctgccg gtaatggaga gcaataattt
    19201 actgcccgtt gccaattggg ccacttactc ccgcctgcaa atgaattatt tagaagccaa
    19261 ggctgcctat gtgcgcgaat ccattaaggg cgaagagaat ttgggcctgg gattaatttg
    19321 ggacggcgat ggtggcaaga attctaatgc ggccttaact attttccgcc ataaagacag
    19381 cgccagtgtg cacaaggggt taattggccc tgcgcccaaa accgcttggg tgattggtta
    19441 cccattgtta gagcgcatcc attatttact ggtggctggg tttgatgtct acggcaattt
    19501 gccccaccaa ttaatgagcc gcttatacat ggattttttg cgcattgaag gcgaaatgaa
    19561 ttttgtggaa ttgctgccga agaaaaccca acaaaccgaa ttggcctttt ggtatcggga
    19621 tgcagaaaat gatctgcagc gctatatcga cgcctattta aaccatttac ccaaccgccc
    19681 actgctcaat taccgcagcg atgagcccaa acaggaatta ttccaagccc tgcgccaaca
    19741 tctggggcag gcggcggcat cgccccacca attaaatccg cagactcaag cagaacacac
    19801 cctggccgct ttgcagatac cgccccaagc acttaaatgg ctgccgcaaa ccactttgct
    19861 gctggtgccg gaggagggcg tatttaccct ggtgcacgtt agcgcctatt ccaatttatc
    19921 ctccttgttc ggtgaggagc gccgccgtcg ccccttggag gatcaattaa ttgtgacgcg
    19981 cggcattatt ggcgcttatc ccaatagttt tttgcgggtg aaccgcaacg atttagcgct
    20041 cttggcggca cagatggcga gtatgaatgc cgaggcggat tatcaggcct taaagcgccg
    20101 ctttggcgtg cgccgcaccg atagtaattt ttgggccaac agcgatgaaa tcgccagcat
    20161 ttttcacaag cagcaacctt cagaagcggc ggtactcgat tacaaccgct tggaaaatcg
    20221 ttgagcattt aaccgcttat ttgtttgtaa ctattcagca cactttactt ccctcttccg
    20281 cgcgggcggc aaattcagga ggatttagcc atccggcaaa atcgattcgc ggtgtgtctg
    20341 cactaacctg cagtcaccac tgcgggcgct ctattacggg ggcacagcac taaaacaccc
    20401 acgcgttagt tcccagtcag tgcaggaatt acctccagcc acacaaaaag atgc
//
LOCUS       DAOQGS010000005        19199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1315_length_19199_cov_4.873955, whole genome shotgun sequence.
ACCESSION   DAOQGS010000005 DAOQGS010000000
VERSION     DAOQGS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 19199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19199
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_19199_cov_4.873955"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3124
                     /locus_tag="PKZ52_00420"
     CDS             <1..3124
                     /locus_tag="PKZ52_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNG58255.1"
                     /translation="VNLQGYVPPSAQSEGLFTRTQWTQIDTGGPEVPILNPEQLNTQV
                     LVPDVSQYTRLSFRLTGVTQDNQVASDTLDLFVYPLANSAKVVGSAQRENTAQVDFKV
                     RLNKPATQTTQLTYLTADGTALAGVDYIPQAASLTFAPGEQEKTVSIRLLSDTTREKG
                     EYFTLQVSGQEGDKPFSARGVAVIRDSDPLPPIVTRKPTQTAVDRAQEELPGLAGQVR
                     VNLQWADAASQIDLMVQDPCNNWLSLNFFTADCQEALGEFEEPSPLVAGRNIVWRTYA
                     PQGRYQVRLHHANGLGSSYTARVYWGDKAAQLEGVMGGGETADIFSFEVTALEAGVPN
                     SATSSSSSSSSSSSSSSSSSSSSSSSSSSSSGTAASSSSSSSASSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSSSSSSSSSSSSSSSSSNSSSSG
                     SSSSSSSSSSSSSSSSTSSSSSSSSSSSSSSSSSSSTSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSSSSSS
                     SSSSSSSSSSSNSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGIVTLGKIY
                     EYNDPLSGSTPGCTLEPTYGAGLGDTQFTRYNLTCPGHQVTLEVTKTTTNGISSCTFL
                     AKPANYSMAGDCNNWRVYYDGPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSGIVTQGKIYEYNDPVSGSTPGCSLEQTYGAGGGDTVFARYNLVCPGHQV
                     TVEITKTTTNGISSCTFLAKPANYSISGDCTNWRVYYDGQSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGTVTQGKIYEHNDPISGS
                     TPGCSLEQTYGAGGGNTVFTRYNLVCPGHQVTVEVTQTTNNGVSNCSFVAKPSNYYIS
                     GDCKNWRVYL"
     gene            complement(3224..3907)
                     /locus_tag="PKZ52_00425"
     CDS             complement(3224..3907)
                     /locus_tag="PKZ52_00425"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNG58256.1"
                     /translation="MRYQHLFAQLFAHRCALCDGASFTALCSACEQDLPRLQACCPQC
                     ALPLAHSEALCPDCLAQPKPFAHTYGAFAYVFPVDSLIQQFKTRAHWPVAAALGCELA
                     AHINARAAAPYTALVPMPQHWRGLLARGRNPAAELATGLGRRLNTPVKPLLRKTRYTP
                     AQKQLTRKERWQNLAGSLACSPLAGGRYLLVDDVMTTGASCILASQVLLDAGAQAVDV
                     AVLARTPER"
     gene            4002..5036
                     /gene="bioB"
                     /locus_tag="PKZ52_00430"
     CDS             4002..5036
                     /gene="bioB"
                     /locus_tag="PKZ52_00430"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNG58257.1"
                     /translation="MSVFSFEPRHNWTKSEVSALFGLPFNDLIFQAQSVHRANFNPNQ
                     VQVSTLCSIKTGACPEDCAYCPQSAHFDTGLEKEKLMAVEKVLEEARAAKASGATRFC
                     MGAAWRSPRDKDMSAVLAMVAGVKSLGMETCMTLGMLDHQQAQQLAEAGLDYYNHNLD
                     TSPEYYGEIITTRTYQDRLNTLANVREAGMKVCCGGIVGMGEAEQDRAGLLLTLANLP
                     EHPESVPINMLVKVEGTPLADAADLDPFDFIRTVAVARIMMPKSHVRLSAGRETMNEQ
                     TQALAFLAGANSIFYGEKLLTTANPEADKDRALFAKLGIMPAPYEVSEEEAVQAPQSR
                     REQLFYNAYN"
     gene            5138..5740
                     /locus_tag="PKZ52_00435"
     CDS             5138..5740
                     /locus_tag="PKZ52_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58258.1"
                     /translation="MGRVSHQTEVFAVVRLLRHLCNQNELNYPIRLYKNERPDFQLHA
                     PNIQIGIEHTEVVCPNVAQLDALRERGKIPDIYFARKSIPGEKKLSAKKLIEEVKNSH
                     LQPPWMGIEPEENWAAAMAHNIYRKDSNYRFQLDAESEQWLILYDNWRAPALDLELAL
                     SKLLELIQFKPIYFFKRMYILSDNNLCCINKGKTSIHAIS"
     gene            5993..6469
                     /locus_tag="PKZ52_00440"
     CDS             5993..6469
                     /locus_tag="PKZ52_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58259.1"
                     /translation="MLEQFKTISDLSEVIKELEGMVLRPTPNWSHPTLYRRRDVSESE
                     KLIQGVRVKDWFMSPDEKWVLPHDQMGLSFSSTYQNLKDVYKLKQRHNKSRQVNIYWI
                     LSAADIPANLAFEADRDKKGHFFLVVTEKMHISSLVTKLKIIARRMSVIRNAEKAL"
     gene            6466..6771
                     /locus_tag="PKZ52_00445"
     CDS             6466..6771
                     /locus_tag="PKZ52_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58260.1"
                     /translation="MINFKFPEEKIYLTHFAEKMGDKKAIAFIQSGRVESEQDAEHLS
                     LFFWRMVDQSIEDEEQNVPRLFNESNEFWNEKIMYSISGYLQRAGFEHIWERVSDAQ"
     gene            7550..8734
                     /gene="bioF"
                     /locus_tag="PKZ52_00450"
     CDS             7550..8734
                     /gene="bioF"
                     /locus_tag="PKZ52_00450"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNG58261.1"
                     /translation="MTDSLTSFLASKLAERKEAHLYRRRLCLDSPQGPEVSEGGRRLV
                     NFCSNDYLSLAAHPKLQEAFSQAASQYGFGSGASHLVCGHSAPHQQLEEALAAFTGRA
                     RALVFSSGYSANLGVITSFLGRADAIFEDRLNHASLIDGGLLSGAAFERFKHNDVGDL
                     TARLAKSTARYKLVAVDGVFSMDGDRAPLVELAELSRAHNAWLMVDDAHGFGVLGPNG
                     GGSTESAGLTAQDVPIYMGTLGKALGCAGAFIAGSEDLIECLIQLARSYIYTTALPPA
                     LAAATCAALALVQQEPERRQRLRGHIAHFTAGAKARNLSLAPSQTAIQPLILGDNARA
                     LAWQQALAARGFWVGAIRPPTVPVGSARLRITLTAGHSRSQIESLLDALAELAHSEAP
                     WI"
     gene            8725..10212
                     /locus_tag="PKZ52_00455"
     CDS             8725..10212
                     /locus_tag="PKZ52_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNG58262.1"
                     /translation="MDLALLEACPNDGRLPLVLLHGWGHNSRIWQAWLEAYPQGRAVY
                     AVNLPGFSEGAEADSAPNLAATLTELEALLPAACMLVGWSLGGMLACQLAQSPKVKGL
                     LTLAANPSFIARPHWPWALAPEVLSNFQSSFAADPSATLQRFSQLQAQGHNNRKALLA
                     SIKALAPEVRPKAWGEALSWLGAIDNSALLAHLNKPQRHLFAEQDALVPATVAAHCPH
                     SRLVPGGHLIPLQGQAELNQTIAALDRAQNRRVAAAFSRAAADYDQYALLQQRLGFEL
                     LEYITPAHQRLLDLGCGTGFMARQLQARPNPPRVINLDLAWGMLQTLPEEAAKVQADA
                     EQLPFAPGAFDAITANLALQWCDLASVLAEAKRCLAPGGQLVFNTLMDGTLAEIAQAW
                     AEVDPYPHINPFPSQSAILAICQQAGFSEVRWQAARHEVQAPSLKALLMGLKGIGAKN
                     KQPARFKGLMSQQHWRAFSDAMRKHGWSGEHWFVSYEVLTLCLRA"
     gene            10245..10739
                     /locus_tag="PKZ52_00460"
     CDS             10245..10739
                     /locus_tag="PKZ52_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58263.1"
                     /translation="MSNHNDVYVYTVTDAGRSRRLASLLPEESTRQHGLKSFAIVGEF
                     TQAEGEEETFTTNPQYIDFLNWAIARHVAHCPGLVAEAQKEQNGHMYITDARGRAQGS
                     ALTEDDIIGVAEIENGKVVRYAGNQNYQPFSRQYGFMVIEPWLNQKIREEMAIQVAGP
                     QQVA"
     gene            complement(11435..11980)
                     /locus_tag="PKZ52_00465"
     CDS             complement(11435..11980)
                     /locus_tag="PKZ52_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNG58264.1"
                     /translation="MKTADINNLLAILQKRFLSHMSRHPTIEWADVQAKLTANTVKLA
                     TLNEMEQTGGEPDLIGFDEARAAYVFCDCAAESPAGRRSLCYDQAALDARKENKPKGN
                     AVATAAAMGIELLTEQDYRHLQSLGEFDKKTSSWIATPQAVRALGGALFCDRRYGQVF
                     TYHNGAESYYAARGFRGLLRV"
     gene            complement(12107..12691)
                     /gene="yfbR"
                     /locus_tag="PKZ52_00470"
     CDS             complement(12107..12691)
                     /gene="yfbR"
                     /locus_tag="PKZ52_00470"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HNG58265.1"
                     /translation="MKSHFYAYISKLRWLRRWGLKRNTVTENVMEHSWEVATIAHALA
                     LIKNRYFGGSLDVNAIVVAAIYHDCSEVITGDLPSPIKYHTREITKAYKAIEHQAELE
                     LLNLLPGDLRQDYEAVIIEANVPDDQRLIIKAADTIAAYIKCRTEVAAGNREFSKAEE
                     AVKARLNNLQQPEVQFFIDTFVGSYALTLDELLN"
     gene            complement(12724..13803)
                     /gene="prfA"
                     /locus_tag="PKZ52_00475"
     CDS             complement(12724..13803)
                     /gene="prfA"
                     /locus_tag="PKZ52_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNG58266.1"
                     /translation="MKASILEKLDSLSERYEEVGVLLGDADIISNQPKFRELSKEYAE
                     LEPVVLAYRDYRKMLNNLAEAKEMLADPEMRAMAQEEINATEALLPALEIDLQKLLLP
                     KDPRDGKNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAETQGWKVEIISESEGEHGGYK
                     EIISRVVGQGVYSQLKFESGAHRVQRVPATESQGRIHTSACTVAVMPEADEADEIAIN
                     KADLRIDTYRASGAGGQHVNKTDSAIRIIHIPTGIVVECQDERSQHKNRAKAMSLLAT
                     KLNNAADEKAAKEISEERKSLVGSGDRSERIRTYNYPQGRVTDHRINLTLYKLDEVME
                     GGALGDVIGPLVNEFQADQLASLGD"
     gene            complement(13800..15098)
                     /gene="hemA"
                     /locus_tag="PKZ52_00480"
     CDS             complement(13800..15098)
                     /gene="hemA"
                     /locus_tag="PKZ52_00480"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNG58267.1"
                     /translation="MTLFALGINHTTATVDLREAVAFSPEQLQQALAEAREHPQLKEV
                     AILSTCNRTEVYAELEGDGSVLLEWLAQFHRAEPAALARCHYLRQGMDAARHMMQVAS
                     GLDSLVLGEPQILGQMKSAYASAVEAKSIGSQLHNTFHKVFSVAKRVRSETAIGENPV
                     SVAYAAVSLAQQIFSDLKQDTALLIGAGETIELVARHLVEAGIKKIIVANRTLINAVE
                     LASQFNGDAILLADIPEHLARADIVIASTASQLPILGKGSVEAALKKRRHKPIFMVDI
                     AVPRDIEPQVADLADVFLYTVDDLKVVIDENMRSREQAAQIAREIIEESVIHYEQELK
                     ALDAKDLLLAYRQSVESARDAELAKALKALASGQAPEEIIQQLARNLTNKFLHKPSAA
                     LKQASGQGNYQFLNDFQNLLELSLEKTLDKPSGIPSSEHE"
     gene            15328..17076
                     /locus_tag="PKZ52_00485"
     CDS             15328..17076
                     /locus_tag="PKZ52_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG58268.1"
                     /translation="MTISLVRPLGLLGLSLGLLACAHRPAGTAPAAPPVAAVATAPEP
                     QPRQYNAFAPETFYSLLVAEVAGNRNRLDVMLSNYQQQAQSTRDPEVVARACRLARFV
                     NQREQALALCSLWVEVSPNLVEARQAALAELLQANRLQEAFAQAEFLVSSGEESGLDV
                     LAARAAQQSVAGTPQLVNDLVPLYENLISRYPSKTEVLLGASYLLQRQNKPDAALEMA
                     RRGLKLKPDDIGLLSQESRLLLGMNRTEEAHSKIKRLLELQPDNQRLRLQYAKSLAAT
                     NLPEAQQQLAVLLNEAPEDSDLNLAMGLVLLEQNKLGEAEPLFKKVSQSRHGGSAQYY
                     LGRIYNQQKDYPRAIEALKAVPPGPDYLPAQGQLILALQKAGRGEEALKELDRAGSQL
                     TPQAQPNIALLKANLLADMGRGDEAIALLTQSLSQNPTSADLLYGRAMIFSRLNNEAA
                     AEVDLRAILASNPNHPATLNALGYGMSKNPARLAEAKGYVEQALQLAPDDPSIMDSLG
                     WIYLQQGKLNEALKLLKQAYSRLADPEIGAHLGEALWRKGNKADAKVIWRQIRSEHPN
                     SEPLRQTLQRLNITLD"
     gene            17107..17709
                     /gene="lolB"
                     /locus_tag="PKZ52_00490"
     CDS             17107..17709
                     /gene="lolB"
                     /locus_tag="PKZ52_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNG58269.1"
                     /translation="MLLRHLLITGLLMFGLTACSRLPQQQPPAAVKSPAELLAQKRYW
                     QAEGKIALSVKDYKESGNFDWQNQGGNFAIRFYGPLGLGAVKLTKEGKIVTFESAKDG
                     THSAESAEELMQRLAGWQVPISQLQHWIKGIPAPGAIESRQDDPAGQLAQLKQLGWTI
                     DYSGYNLVNGWLLPGKLIASREQVRVVMVIKNWQLTPVGE"
     gene            17711..18550
                     /gene="ispE"
                     /locus_tag="PKZ52_00495"
     CDS             17711..18550
                     /gene="ispE"
                     /locus_tag="PKZ52_00495"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNG58270.1"
                     /translation="MLRLPCPAKLNLFLHILGRRGDGYHQLQTLFQLLDYGDELQVSA
                     TNDGAIRLENPLPGVADEDNLIIKAARLLKEHTGCSFGARLALLKRLPMGGGIGGGSS
                     DAASTLLGLNHLWGLNLSLDELAALGRRLGADVPVFVHGRSAWAEGIGEELTPVELPE
                     RWFVVLAPKAHVSTAEIFQHKRLTRDSAPITMRAFLEEGGRNDCQPLVEELFPDVKDA
                     VGWLKQFSAAQLTGTGGCVFASFASQEQAERVMLERPGQLNGFWAKGVNESPLHSYLR
                     RCQ"
     gene            18557..18631
                     /locus_tag="PKZ52_00500"
     tRNA            18557..18631
                     /locus_tag="PKZ52_00500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18589..18591,aa:Gln,seq:ttg)
     gene            18716..>19199
                     /gene="prs"
                     /locus_tag="PKZ52_00505"
     CDS             18716..>19199
                     /gene="prs"
                     /locus_tag="PKZ52_00505"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNG58271.1"
                     /translation="MVFSGNANPPLAQKVVEKLGIPLGDATSSQFSDGEISIEINENV
                     RGRDVFVMQSTCVPTNDNLMELLFMVDALRRASAGRITAVLPYFGYARQDRRVRSARV
                     PISAKVVADMMATAGVDRVLTVDLHAEQIQGFFDVPVDNVYGSPVLLADIELRKFENL
                     V"
BASE COUNT         4392 a         5620 c         5052 g         4135 t
ORIGIN      
        1 ggtgaacctg caaggctatg tgccgccaag cgcccagagt gaaggcctat tcacccgcac
       61 ccagtggacg caaattgata ccggcgggcc agaggtgccg attctcaacc cggagcaact
      121 caacacccaa gtgctagtgc ccgatgtcag ccagtacacc cggctgagtt ttcgccttac
      181 gggtgtcacc caggacaacc aagtggcctc ggataccctg gacctgttcg tataccccct
      241 cgccaatagc gccaaagtag tgggcagtgc ccaacgggaa aacaccgctc aggtggactt
      301 taaagtgcgc cttaataaac cagccaccca aaccactcag cttacctacc tcaccgccga
      361 cggcaccgcc ctggcggggg ttgattacat cccccaagcg gccagcctaa cctttgcccc
      421 cggcgagcag gagaaaaccg tctcgatccg gctgctaagc gacaccacgc gggaaaaggg
      481 tgagtacttc accctgcaag tgagcgggca ggaaggtgac aagcctttta gcgcccgcgg
      541 tgtagccgtg attcgcgaca gcgacccatt gccgccgatt gtcacccgca aacccacgca
      601 aactgccgtg gatcgtgccc aagaggagct gccaggcctt gctggccaag tgcgggttaa
      661 cctgcaatgg gcggacgcgg caagccagat cgatctgatg gtgcaagacc cttgcaacaa
      721 ctggctgagc ctcaacttct tcaccgccga ctgccaagag gccctgggtg aatttgaaga
      781 gccaagccca ctggtagccg ggcgcaatat cgtctggcgc acctacgcac cccaagggcg
      841 ctaccaagtg cgcctgcacc atgctaacgg gctgggctct agctataccg ctcgggtcta
      901 ctggggcgac aaggcggccc agctcgaagg cgtcatgggc ggcggggaga cggcggatat
      961 tttcagcttt gaggtgacgg ccctggaagc cggggtgccg aattcagcaa cgagttcttc
     1021 ttcgagtagt tcaagctcgt cttcgagtag ttcgagttct tcttcgagta gttcgagctc
     1081 ctcttcgagc agctctggca cggcggcgag ttcatcaagc tccagttcgg ccagctcttc
     1141 gtcaagctca agcagttcta gctcgtcttc aagtagcagc tctagctcca gctcctcatc
     1201 gagttcaagc tcatccagca gcagctcatc atcaagttcg agcagctcca gtagctcttc
     1261 cagcagcaca agctcatcga gtagctcttc ttccagtagc tccagttcgt cgagcagcag
     1321 ctctagctct tcaaacagct catcgtctgg ctcgagttca tcctcaagct caagttcttc
     1381 ctccagttct agctcatcca catcgtcgag cagctctagc tcgtcaagca gttctagctc
     1441 ttcttccagc tcttcctcta ccagcagctc aagctcttca agcagctcgt cgtctagctc
     1501 gagttcatcc tcaagctcaa gctcttcttc aagttctagt tcatccacat cgtcgagcag
     1561 ctccagctcg tcaagcagtt ctagctcttc ttccagctct tcgtctagca gtagctcaag
     1621 ctcttcaagc agctcgtcgt ctagctcgag ttcatcctca agctctagct cgtcttcaag
     1681 ttccagttca tccacatcgt cgagtagctc cagctcgtca agcagttcta gctcttcttc
     1741 cagctcttcg tctagcagta gctcaagctc ttcaagcagc tcgtcgtcta gctcgagttc
     1801 atcctcaagc tctagctctt cttcaagttc cagttcatcc acatcgtcga gcagttcaag
     1861 ctcctcttca agctctagct cctcgtctag ctcgagttca tcctcaaatt ccagctcgtc
     1921 ctcttcaagc tctagctctt cgtccagctc aagtagctca tcgtcgtcca gcagttcctc
     1981 aagtagctca tcttccagca gctcgtcctc ttcaagctcc ggcatagtca ccctgggcaa
     2041 aatttatgaa tacaacgatc ccctcagcgg ctcaacgcca ggctgtacgc tggaaccaac
     2101 ctacggtgca ggcttagggg acactcaatt cacccgatac aatctgacct gcccaggtca
     2161 tcaggtaacc ctggaggtaa ctaagacaac caccaatggg ataagtagtt gtaccttcct
     2221 ggcgaaacct gccaactatt ccatggccgg tgactgtaac aattggcggg tttattacga
     2281 tggacccagt tccagtagct cgtcttcaag ctcctcgtca agttctagct cttcgtccag
     2341 ctcgagtagc tcatctagct cctcgtccag cagttcctcc agcagcagct cgtcttccag
     2401 cagttcgtcg tcctcaagct caggcatagt cacccagggc aaaatttatg agtacaacga
     2461 tccggtcagt ggctcaacgc ctggctgctc gcttgagcag acctacggtg caggcggcgg
     2521 cgacactgtg ttcgcacgct acaacttggt gtgtccgggc catcaggtaa cggtggagat
     2581 aactaaaaca acgactaacg ggataagtag ttgtaccttc ctggcaaaac ctgccaacta
     2641 ctcaatttca ggcgattgca ccaattggcg ggtttattac gatgggcaga gttccagtag
     2701 ctcgtcctct agctccagca gctcaagtag cagctcgtcc tcgtcctcaa gctcctcgtc
     2761 aagttctagc tcttcgtcca gctcgagtag ctcatctagc tcatcgtcca gcagttcctc
     2821 aagcagcagc tcgtcatcca gcagttcgtc gtcctcaagc tcgggcacgg tcacccaggg
     2881 caaaatttat gagcacaacg atcccatcag cggttcaacg cctggctgct cacttgaaca
     2941 gacctacggt gcaggcggcg gcaacactgt gttcacacgc tataacttgg tgtgcccagg
     3001 tcatcaggtg acggtggaag tcactcaaac gactaacaat ggggtaagca actgctcttt
     3061 cgtggccaaa ccttcgaatt actatatttc cggggattgc aaaaactggc gggtatacct
     3121 gtaaatcctg ttagacccgg cacaagaata ctgggaagga tctaacaaac cgattggcag
     3181 aggtaacttt ggggtttagc aattgcgcgt cataccccaa agctcaccgc tccggcgtgc
     3241 gcgccaacac tgccacatct accgcctggg cccctgcgtc caacaacacc tggctcgcca
     3301 gtatgcaact ggcgccggtg gtcatcacat catccactaa taaataacgc ccgcctgcca
     3361 ggggcgagca cgctaggctg ccggcgaggt tctgccagcg ctccttgcgg gtgagctgtt
     3421 tttgcgcggg ggtgtagcga gttttgcgca gcaggggctt aaccggtgta ttgagcctgc
     3481 ggcctaaacc ggtagccagc tccgccgccg ggttgcggcc gcgggccaac aggccccgcc
     3541 aatgctgagg catgggcacc agggcggtgt agggggcggc ggcgcgggcg ttgatgtggg
     3601 ccgccaattc gcaccccagg gcggcggcca ccggccagtg ggcgcgggtt ttaaactgct
     3661 ggatcaggct atccaccggg aacacatagg caaaggcccc ataggtgtgg gcaaacggtt
     3721 taggctgggc cagacaatcg gggcagaggg cttcgctatg ggcgagcggc agggcgcatt
     3781 gggggcaaca ggcctgcagg cggggtaagt cctgctcgca agcgctgcac agggcggtga
     3841 aacttgcgcc atcgcagagg gcgcagcggt gggcaaaaag ctgggcgaac aggtgttgat
     3901 aacgcatagt aaactggata ctccttattt ggttgacaac tgcttgttgg cctttaagat
     3961 gcctgcataa cccccgccgg cacccaagaa aaggcccctc tatgtccgtt tttagcttcg
     4021 aaccccgcca caactggacc aaaagcgaag tgagcgccct ttttggcctg cccttcaacg
     4081 acttaatctt ccaggcccaa agcgtgcacc gggccaactt taaccccaat caggtgcagg
     4141 tgagcaccct gtgctccatc aaaaccggcg cctgcccgga agactgcgcc tactgcccgc
     4201 aaagcgccca ttttgacacc ggcctggaga aagaaaaact tatggcggtg gaaaaggtgc
     4261 tggaagaagc ccgcgccgcc aaggcctccg gcgccacccg cttctgcatg ggcgccgcct
     4321 ggcgctcccc ccgcgacaaa gacatgagcg cagtattggc aatggtggcc ggggttaaat
     4381 ccttaggcat ggaaacctgc atgaccctcg gcatgctcga ccaccagcag gcccagcagc
     4441 ttgccgaagc cgggctggat tattacaacc acaacctgga tacctcgccg gagtattacg
     4501 gcgagatcat caccacccgc acctaccaag accgcctcaa caccctagcc aatgtgcgcg
     4561 aggcgggtat gaaggtgtgc tgcggcggca tagtgggcat gggggaagcc gagcaagatc
     4621 gcgcaggatt gctgctcacc ctggccaacc tgccggaaca cccggaatcc gtgcccatta
     4681 atatgctggt aaaagtagag ggcacacccc ttgccgatgc ggcggattta gacccgttcg
     4741 attttatccg caccgtggcc gtagcccgca tcatgatgcc caaaagccac gtgcgcctgt
     4801 ccgccgggcg ggaaaccatg aacgaacaaa cccaggccct ggcgttttta gcgggggcga
     4861 attcgatttt ttatggggaa aaattattga cgaccgcaaa cccggaggcg gataaggacc
     4921 gggctttgtt tgcgaaattg gggattatgc cagcgcctta tgaggtgagt gaggaggagg
     4981 cagtgcaggc gcctcaaagc cgtcgagaac agctttttta taacgcttac aactgaataa
     5041 taaatgcttc ctagagtcct caatgattac agcaaacaca agacaggaac ttatttcaac
     5101 gctcgagaat ttcaatgcgg aggtttcacg gagatctatg ggcagagttt cccatcaaac
     5161 ggaggtgttt gccgtagtta gactactcag gcatttatgc aatcaaaatg aattaaatta
     5221 ccccatacgc ctttataaaa atgaacgccc ggatttccaa cttcacgccc ccaatatcca
     5281 aataggcatc gaacataccg aagttgtttg tccaaacgtc gcacaactgg atgcactacg
     5341 agaaagaggg aaaatcccag acatttattt cgcaagaaaa tccattccgg gcgagaaaaa
     5401 actatcagca aaaaaactaa tcgaagaagt taaaaacagt caccttcaac caccctggat
     5461 gggcattgaa cccgaagaga actgggccgc cgcaatggca cataatatat acaggaagga
     5521 ttccaactac agatttcaac ttgatgcaga aagtgagcag tggctaatac tttatgacaa
     5581 ttggcgcgct ccagcactag atctggaact agccctatca aaactattgg agttaattca
     5641 attcaagcct atctattttt ttaagcgcat gtatatctta agcgacaata atctgtgttg
     5701 tatcaacaaa ggaaaaacct caatccatgc tattagttga tttggcattg cgagacagct
     5761 ctttagagcc tagcagttga atcaacagca accactataa atcagcgccc ttgatcgctt
     5821 gagacaagga agcctcaatt ttgcaccagg atacaatctg ctgtgcaagc tactcttgtt
     5881 agcgaatctg ccgttatatt tatttttata gattataaaa acacccacaa ctcaatcagc
     5941 caccattagc cgaaccgttt aattagggct aaataaaatt ggagccatag tcgtgctaga
     6001 gcaattcaag accatcagtg acttgagtga agttatcaag gaactagagg gcatggttct
     6061 tcgccccaca cctaattgga gccatccaac tctatatcgg cgtcgagatg tttctgagag
     6121 tgaaaaatta attcagggcg taagggtaaa agactggttt atgtctcctg acgaaaaatg
     6181 ggtgctgcct cacgaccaaa tgggactttc attttcttcc acttatcaaa acctcaaaga
     6241 cgtatataaa ctaaaacagc gccacaacaa gagcaggcaa gttaatattt attggatact
     6301 tagtgcggcc gacatccctg caaaccttgc ttttgaagca gacagagata aaaaggggca
     6361 cttttttcta gtcgttacag aaaaaatgca tatatcaagc cttgtcacca agctaaaaat
     6421 tattgcacgc agaatgtcgg ttatacgcaa tgcggagaag gcactatgat caactttaaa
     6481 ttccccgaag aaaaaatcta cctcactcac ttcgcagaaa aaatgggcga caaaaaagca
     6541 attgctttta tccaatccgg cagagtggaa tccgaacaag atgccgaaca tttaagcctg
     6601 tttttctggc gcatggtcga tcaatccatc gaagacgaag agcagaatgt cccgcgctta
     6661 tttaatgaat ccaacgaatt ttggaatgaa aaaattatgt attcaatttc cggttatttg
     6721 cagcgggcgg gttttgaaca tatttgggag cgagtttctg atgcgcagta gtgttggcat
     6781 aggccccgac acggccacac atctacccac cccacacagc cactttaata accaccgaag
     6841 ccaatgaact ctaaccaact cataatttgc gctattttgc ttattggcct agccgctgca
     6901 ctttatgcgt ttataaaaac acccccctcc caacgctggg cgctatcaag gccgccggag
     6961 gccaaaaaag aaggggttga atatgccccc atgtatagcg acagcgaacg acgaaaaatt
     7021 gccattattc acatcagctg gctcattcct gcatgtgcct tttttcaatt ttacctgctg
     7081 ccaaaataca aagtgtttgc agccaatgct gcctgttacg aaattgcgac cataaatggc
     7141 acaaaaatat tgttttttgg gcttttgatt ggggggccat tattgggcgc catcatgaca
     7201 tttgctaccc agggtatagc gtcgctgcgc gcattgcgtg cggggcaatt tcccgcccct
     7261 aacgtaaaag tctttcggct aactccatat aaatatggcg tcgctgccaa aattcctgcc
     7321 ctatttgttt ttgtcgttat tgcgcttctt ttaggaatga gcctgtgggg ttactacgtg
     7381 gcaagcggcc taacccaaca ggtaacaacc tgccaaacgc cccataccaa aactggacta
     7441 aacccatgaa tggctccgta gggcgcacct gggtgcgcca catcctaaac tccaagcgat
     7501 accaactggc gcaccaaggt gcgccctacg cccctgttgg gcatttaaca tgaccgactc
     7561 cctaaccagc ttcctcgcca gcaaactcgc cgagcgcaaa gaggcccacc tctatcgccg
     7621 ccgcctgtgt ttagactccc cccaagggcc cgaggtaagc gagggcgggc gccgcttggt
     7681 gaatttttgc agcaacgatt atttaagcct cgccgcccat cccaagttac aggaggcctt
     7741 tagccaggcg gccagccagt atggctttgg ctccggcgca tcccatttgg tgtgcggcca
     7801 cagcgcgcca catcagcagt tggaggaagc cctggcggct tttaccgggc gggcccgggc
     7861 gctggtgttt tccagcggtt acagcgccaa tctgggggtt atcaccagct ttttgggcag
     7921 ggctgatgca atttttgaag accgcttaaa tcacgccagc ctgatcgacg gcgggctttt
     7981 atccggcgcc gccttcgagc gttttaaaca caacgatgtg ggcgatctaa ccgctcgcct
     8041 ggcaaaatcc actgcccgct ataagctggt ggcggtggat ggggtgttta gtatggatgg
     8101 cgatcgggcg ccgctggtgg aattggcgga gcttagccgc gcccataacg cctggctgat
     8161 ggtggacgac gcccacggct ttggtgtgct cggccccaat gggggcggca gcacggagtc
     8221 ggcggggctt accgcccagg atgtgcccat ttatatggga accctgggca aagccctggg
     8281 ctgcgccgga gcttttattg cgggcagtga agaccttatc gaatgcctta ttcaactggc
     8341 gcgctcctat atttacacca ccgccctgcc cccggccctg gccgccgcca cctgcgccgc
     8401 ccttgcgcta gtacagcagg aacctgaacg acggcagcgg ctgcgggggc atatagccca
     8461 cttcaccgcc ggggccaagg cgcgcaacct aagcctcgcc cccagccaaa ccgctatcca
     8521 gccattaatt ctgggggaca acgcccgcgc cctcgcctgg caacaggcgc ttgccgcgcg
     8581 gggtttttgg gtgggcgcca tccgcccacc gacggtgccc gtaggcagcg cccgcctgcg
     8641 catcaccctc accgcaggcc acagccgcag ccaaattgaa agcctgctgg atgccctcgc
     8701 cgaactagcc cacagcgagg caccgtggat ttagcactgc ttgaggcctg ccctaatgat
     8761 gggcgcctgc ccctggtatt gctgcacggc tggggccata acagccgcat atggcaagcc
     8821 tggcttgagg cctaccccca gggccgtgcg gtgtatgcgg taaatctgcc gggttttagt
     8881 gaaggcgccg aggctgacag cgcaccgaac cttgcggcca ctctgactga acttgaagcc
     8941 ctcttacctg cggcctgtat gctggtgggc tggtcgttgg gcggcatgct cgcctgccaa
     9001 ctggcccagt cccccaaggt gaagggtctg ctcaccctgg ccgccaaccc cagttttatc
     9061 gcccgccccc actggccctg ggcactggcc ccggaggtgt taagcaattt ccaaagcagt
     9121 tttgctgcag accccagcgc caccctgcag cgctttagcc agttgcaggc ccagggccac
     9181 aacaaccgca aggcgctgct ggcaagtatc aaagcgctag caccggaggt gaggcccaag
     9241 gcctggggtg aggccttgag ctggctcggc gccatagata acagcgcttt acttgcacat
     9301 ctcaacaaac cccagcgcca cctatttgcc gaacaagacg ccctggtgcc cgccaccgtc
     9361 gccgcccatt gcccccacag ccgcctggtg cccggcggcc acttgattcc cctgcagggc
     9421 caagccgagc taaaccagac catcgccgcc ctggatcgcg cccaaaaccg ccgtgtggcc
     9481 gccgccttca gccgcgccgc cgccgactac gatcaatacg ccctgctgca acagcgcctg
     9541 gggtttgagc tgctcgaata cataaccccc gcccaccagc gcctgttgga cttaggctgc
     9601 ggcaccggtt ttatggcccg ccagttacag gcccgcccga acccaccgcg tgtgatcaac
     9661 ctggatctag cctggggcat gttgcaaact ctgccggagg aggccgctaa agtacaggcg
     9721 gatgccgaac aactgccctt tgccccaggc gcctttgacg ccatcaccgc caacctggcc
     9781 ctgcagtggt gtgacctggc cagcgtactg gcggaggcca aacgctgcct ggcccccggc
     9841 ggccaattgg tgtttaacac cctgatggat ggcaccctgg ctgaaatagc ccaggcctgg
     9901 gccgaggtgg acccctaccc ccatatcaac ccgttcccca gccaaagcgc gatactcgcc
     9961 atctgccaac aggcaggttt tagcgaggtg cgctggcagg ccgcccgcca tgaggtgcag
    10021 gccccaagcc ttaaggcctt gctcatgggc ttaaaaggca taggcgcgaa gaacaaacag
    10081 cccgcacgct ttaaaggcct gatgagccag cagcactggc gcgcctttag tgacgccatg
    10141 cgcaaacacg gctggagtgg cgagcactgg tttgtcagct acgaggtttt aaccttatgc
    10201 ttgcgggcct aaccatttct cccccctcgc gagtcctttt tgccatgtcc aaccacaacg
    10261 atgtttacgt ctacaccgtc accgacgcag gccgctcccg ccgcctcgcc tctctcctac
    10321 cagaagagtc cacccgccaa cacggcctga aaagcttcgc catagtgggg gaattcaccc
    10381 aggccgaagg cgaagaagag acattcacca ccaaccccca atacatcgac tttttaaact
    10441 gggccatcgc ccgccatgtg gcccactgcc caggcctggt ggccgaagca caaaaagaac
    10501 aaaacggcca tatgtacatc accgacgccc gcggccgcgc ccaaggtagc gcactgactg
    10561 aagacgatat tatcggtgtg gcggaaattg aaaacggcaa ggtggtacgc tacgcgggca
    10621 accaaaacta ccagcccttc agccgccaat atggatttat ggtgatcgag ccctggctga
    10681 atcaaaaaat tcgcgaggaa atggcgattc aggtggcagg gccacagcag gtggcttagt
    10741 ggttttatag gtccgtgtta aatcctaacg ggaataactg caaattaatt gacgggaatt
    10801 ttgcaatacg gccatagcaa tacccccgcc cttacaatgc atcccgccgg aagcaatacc
    10861 attaagttaa acagttaagg atttttgcag cctgccccac cctaggattc ccctcctatg
    10921 gaggggtggc aggcgtgcct gtcgcggtgg cgggcgcttt gctttttcac cggacgagga
    10981 aattttttcc tgttttttgc aaaaaagaag acgggctgcg aaccaaaaat agcgcagcga
    11041 gctgcaaacc ccagcggggg aatacttcca cagcaatgtc actaagctca gcgttttgtc
    11101 ttgtatttta cctggttttg tgcctccccc taaaccccaa ccctcccctg ttaaagggga
    11161 gggagcaaga gtgtctggtg tacgagcaaa atgcctgggt ttaaaggttt ttactcctcc
    11221 ccttttggag ggggagaccg ggagggggtt tttaggggcg agaaaagtgc attaaataca
    11281 ggctcaaata cttaacttaa tgatgctgct ctctacagga tgggaatttt acgctctgcc
    11341 catgaccaaa aaagcccctc gttttaaggg cattgcctct tgaaggagag ggatttttat
    11401 aggcgtggcg gcttttgtgc cgggttaata acactcaaac ccgcaacaag ccccgaaacc
    11461 cccgcgccgc ataataggat tccgccccat tgtggtaggt aaacacctgc ccgtagcggc
    11521 gatcgcaaaa taacgcgccg ccgagggcgc gcactgcttg cggagttgct atccagctag
    11581 aagttttttt atcgaattcg ccgagggatt gcagatggcg ataatcctgc tcggttaata
    11641 attcaatgcc catggccgcc gccgttgcta ccgcattgcc cttgggttta ttttctttac
    11701 gggcatctaa agcagcttgg tcgtaacaca ggctgcgccg ccccgctggg ctctccgctg
    11761 cgcagtcgca aaaaacgtat gcagcgcggg cttcatcgaa gcctataaga tccggctcgc
    11821 caccggtctg ctccatttca ttaagtgtcg ctagtttcac tgtattcgcc gtaagttttg
    11881 cctgcacatc cgcccactca atggttggat ggcgagacat atggcttaaa aaacgttttt
    11941 gcagaattgc caggagatta ttgatatctg cggttttcat agggccctgc cgaaattaac
    12001 gatttttact tgctgcggaa aatttctcgt tcccacgctc tgcctggaaa caaaaagaaa
    12061 cgaaaagaaa cgaaaagaaa cgagaagaag gtaggaaaac aaaatatcaa tttaataact
    12121 catccaaagt aagcgcataa ctgcccacaa aggtgtcgat aaaaaattgc acctcaggct
    12181 gctgcaaatt attgaggcgc gcttttaccg cctcctccgc cttgctaaat tcccgattgc
    12241 ccgccgccac ttcagtgcgg cacttaatat aagcggcgat ggtgtcggca gctttaataa
    12301 tcaggcgctg atcatcaggc acatttgcct cgataatgac cgcctcgtaa tcctgccgca
    12361 aatcccccgg caataaattg agcagctcta attccgcctg atgctcgatg gctttatagg
    12421 ctttggtgat ttcgcgggtg tggtatttaa tgggggaagg taaatcgccg gtaatcactt
    12481 cgctgcagtc gtggtaaatg gccgccacta ctatggcgtt cacatccaaa ctgccgccga
    12541 aataacggtt tttaatcagc gccaaagcat gggcgatggt ggccacttcc cagctgtgct
    12601 ccatcacatt ttctgtcact gtattgcgct ttaaacccca gcggcgcagc cagcgcaatt
    12661 tactgatgta cgcgtaaaag tgacttttca tggcagcacc gctgaattat aaaaactaaa
    12721 aaattaatcc cccagggacg ccaactgatc cgcctggaat tcattcacca aaggcccaat
    12781 tacatcgccc agggcgccgc cttccatcac ttcatccaat ttatacaggg ttaaattaat
    12841 gcggtggtcg gtaacgcggc cctgggggta gttataggtg cgaatgcgct cggagcgatc
    12901 ccccgacccc accaggcttt tgcgctcttc actgatttct ttagcggctt tttcatcggc
    12961 ggcgttgttt aatttagtgg ccaataggct catggcctta gcgcggtttt tatgttggct
    13021 gcgctcgtct tggcattcca ccactatgcc ggtggggatg tgaataatgc gaatagccga
    13081 gtcggttttg ttcacgtgct ggccaccggc gccgctggcg cggtaggtat caatgcgcaa
    13141 atccgcttta ttaatggcaa tttcatcagc ttcatcggcc tcgggcatta ccgcaaccgt
    13201 gcacgccgaa gtgtgaatgc ggccttggga ttcggtggcg ggcacccgct gcacccggtg
    13261 ggcgccagat tcgaatttta attgcgaata aaccccctgg cctaccacgc gggagataat
    13321 ttctttataa ccgccgtgtt cgccctcgct ttcggaaata atttccacct tccagccttg
    13381 agtttcggcg taacgcgaat acatgcgaaa taaatcaccg gagaaaatcg ccgcttcgtc
    13441 gccacctgtg cccgcgcgaa tttccaaaaa gacgtttttg ccatcgcggg ggtctttggg
    13501 caataaaagt ttttgcaggt caatttctag ggcgggcagc agggcttcgg tcgcgttaat
    13561 ttcttcctgg gccatggcgc gcatttcggg gtcggcgagc atttctttgg cttcagccaa
    13621 gttgttaagc attttgcggt aatcgcgata ggcgagcaca accggttcta attcggcgta
    13681 ctctttggag agttcacgaa attttggttg attgctaata atatccgcat cgcccagcaa
    13741 tacgccgact tcttcataac gctcggagag gctgtcgagt ttttctaaaa ttgaggcttt
    13801 cattcgtgct cgcttgaggg tattccgctg ggtttatcga gggttttttc cagcgacaat
    13861 tccagcagat tttgaaaatc atttaaaaat tgataattgc cctggccgct ggcctgttta
    13921 agtgccgcgc tgggtttgtg taaaaattta ttagtgagat tgcgcgctaa ttgctgaatt
    13981 atttcctctg gcgcttggcc gctggcaagg gctttgaggg ctttggcaag ttcggcgtcg
    14041 cgggcgcttt cgacgctttg gcgataggct aataacaggt ctttagcgtc gagggcttta
    14101 agttcctgct cgtagtgtat aacactttct tcgatgatct cgcgggcaat ttgcgcggcc
    14161 tgttcccgcg agcgcatgtt ttcatctatg acaactttaa gatcatccac cgtgtataaa
    14221 aacacgtcgg ctaagtccgc cacctgaggt tcaatatccc gtggcacggc gatatccacc
    14281 ataaaaatcg gcttatggcg gcgttttttt aaggccgctt ccaccgagcc cttgcctaaa
    14341 atcggcagct ggctcgcggt ggaggcaata actatatcgg cgcgggctag gtgttcggga
    14401 atatccgcta ataaaatagc gtcgccatta aattggctgg ccaactccac cgcattaatg
    14461 agcgtgcggt tggcaacgat aattttttta atgcccgctt ccaccaaatg gcgggccacc
    14521 agttcgatgg tttcaccggc gccaattaga agcgccgtat cctgttttaa atcggaaaat
    14581 atctgttggg cgaggcttac cgccgcatag gctacggaga cggggttttc gccgatggcg
    14641 gtttctgagc gcacccgctt ggcgacggaa aataccttgt ggaacgtatt gtgtaattgg
    14701 ctgccgatac ttttcgcctc tacggcgctg gcgtaggcgg atttcatctg gccgagaatt
    14761 tgcggctcgc ccagcaccaa agaatccaaa ccgctggcca cctgcatcat gtggcgggcc
    14821 gcgtccatgc cttggcgcag gtagtggcag cgggccaggg cggcaggctc cgcgcggtga
    14881 aattgcgcca gccactccag cagcacgctg ccatccccct ccagctcggc ataaacttcc
    14941 gtgcggttac aggtggagag tatggcgact tcttttagct gcgggtgctc gcgggcctcg
    15001 gccaatgcct gctgcaactg ctcggggctg aaggcgacgg cctcccgcag atccacagtg
    15061 gcagtggtat ggttgatacc caaggcaaat aaggtcatgg cagtgtttgg tagtggtgga
    15121 gcatggaggt cccaacggaa attggccaaa gcgctaagcc ctggcacctt aaacccccac
    15181 cgtctatgat gcgcgcagcg gcaaacctac gatcgggtgc aggattatag gcggcaagcc
    15241 cgattgcgcc tagccaggcg gcaccgcctt tactgtctaa actctgccaa tccccaaatc
    15301 aaacccaaaa acgtgattca cttacccatg acaatctccc ttgtgcgccc cctcgggttg
    15361 ttaggcttaa gcctcggcct tttggcctgc gcccaccgcc cagcgggcac agcaccagcc
    15421 gctccgccgg ttgctgcggt cgcaaccgca ccagagccgc agcctcgcca gtacaacgcc
    15481 tttgcgccgg agacctttta ttctctgttg gtggcggagg tggcgggcaa tcgcaaccgg
    15541 ctggatgtga tgctaagtaa ttaccagcaa caggcccaaa gcacccggga cccggaagtg
    15601 gtggcgcgcg cctgccgctt agcgcggttt gtgaatcagc gcgagcaggc cctagcgctg
    15661 tgctccttat gggtggaggt gagcccgaac ctggtggagg cccgtcaagc ggccctggcg
    15721 gagttgctgc aggccaaccg cttacaggag gccttcgccc aggcggagtt tttagtcagc
    15781 agcggggagg agtcgggcct ggacgtgctc gccgcccgcg ccgcccagca atccgtggcg
    15841 ggcaccccgc aattggtgaa cgacctggtg cccctgtacg aaaatttaat cagccgctac
    15901 ccgagtaaaa ccgaagtgct gctcggcgcc agttatctac tgcaacggca gaataagccg
    15961 gatgccgccc tagagatggc ccgccggggc ctcaaactta agcccgacga tataggcctg
    16021 ttgagtcagg agtcgcggct gttgctgggc atgaaccgca ccgaggaggc ccacagcaaa
    16081 atcaaacgcc tgctggagtt gcaacctgac aaccagcgcc tgcgcctgca atacgccaag
    16141 agcctggccg ccaccaacct gccggaggcc cagcagcagc tggcggtgtt attaaatgaa
    16201 gcgccagagg atagcgacct caacctggcc atgggcctgg tgctgctgga gcaaaacaaa
    16261 ctcggcgagg ccgaaccgct gtttaaaaag gtcagccagt cccgccatgg gggctccgcc
    16321 cagtattatc tgggccgtat ttacaaccag cagaaggact acccccgcgc catcgaagcg
    16381 cttaaagcgg tgccgccggg gccggattat ttgcccgccc agggccagct gattctcgcc
    16441 ctacaaaagg ccggtcgcgg tgaagaggcc ttaaaagagc tggatcgcgc cggcagccag
    16501 ctgacgcccc aggctcagcc caatatcgcg ctgttaaagg ccaatttact ggccgatatg
    16561 gggcgaggcg acgaggccat cgcgctgctt acccaaagcc tcagccaaaa ccctacttca
    16621 gcggacctgc tctatggccg ggcgatgatt ttcagccgcc tcaataatga agcggccgcc
    16681 gaggtggacc tgcgggcgat tttagccagc aaccccaacc accccgccac cctgaacgcc
    16741 ctgggttatg gtatgtcgaa aaaccccgcc cgcctggccg aggccaaggg gtacgtggag
    16801 caggccctgc aattggcgcc ggatgatccc tccatcatgg acagcctagg ctggatttac
    16861 ctgcagcagg gcaaactcaa cgaggcccta aaactcctca agcaggccta tagccgcttg
    16921 gcggacccag agatcggcgc tcacctgggg gaagccctct ggcgcaaggg caataaagct
    16981 gacgccaagg tgatttggcg gcagatccgc agcgagcacc ccaacagtga acccctgcgc
    17041 caaaccctgc aacgcctcaa catcaccctg gactagccca gggctttata tcgattggat
    17101 tagctcatgt tattgcgtca tcttttgatc accggcctgc tcatgtttgg cctcaccgcc
    17161 tgtagccgcc tgccccaaca acaaccgcct gccgccgtga aaagccccgc tgagctgctg
    17221 gcgcaaaaac gctactggca ggcggaaggc aaaatcgccc tgagcgtgaa agactacaaa
    17281 gagagcggca acttcgactg gcaaaaccag ggcggcaact tcgctatccg cttttatggg
    17341 cctctggggc taggcgccgt caaactcacc aaagagggca aaatcgtcac ctttgaaagc
    17401 gccaaagacg gcacccacag cgccgaaagc gccgaggaac tgatgcagcg cctggcgggc
    17461 tggcaggtgc ccatcagcca gttacaacac tggattaaag gcatcccagc ccccggcgcc
    17521 attgagtccc gccaggacga cccggccggg cagcttgcgc agcttaaaca actgggctgg
    17581 accatcgact acagcggcta caacctggta aacggctggc tgctgccggg caagttaata
    17641 gccagccggg agcaggtgcg ggtagttatg gtgattaaaa actggcagct gaccccagtc
    17701 ggtgaatagc atgctgcgcc tgccctgccc cgccaaactc aatttattcc tgcacatcct
    17761 cggccgccgc ggcgatggct accaccagtt gcagaccctc tttcagctgc tggactacgg
    17821 cgacgaactg caggtgagcg ccaccaacga cggcgcaata cgtttagaga accccctgcc
    17881 cggggtagcc gacgaggaca acctcatcat caaagccgcc cgcctgctta aagaacacac
    17941 cggctgctcc ttcggggcca ggctcgcact ccttaagcgc ctacccatgg ggggcggcat
    18001 aggcggcggc agctccgatg ccgccagcac cctactcggc cttaaccacc tatggggatt
    18061 gaacctgagc ttggatgaac tcgccgccct cggtcgccgc ctgggggcgg acgtgcccgt
    18121 gtttgtgcat ggccgcagcg cctgggcgga gggcattggc gaggagttaa cgccggtgga
    18181 actgcccgag cgctggttcg tggttttggc ccccaaggcc catgtaagca cggcggaaat
    18241 ttttcagcac aagcgtttga caagagattc cgcgcccatt accatgcgcg ccttccttga
    18301 ggagggtggg agaaatgact gtcaaccatt ggttgaagag ctcttccccg acgtgaagga
    18361 tgcggttggt tggctaaagc aatttagcgc agcacagctc acgggaaccg gcggttgtgt
    18421 gtttgcaagt tttgcatcgc aagaacaagc cgagcgagtg atgctagagc gaccgggaca
    18481 actgaatggc ttttgggcca aaggtgtgaa cgaatcgcca ctgcactctt acctccgaag
    18541 atgtcaatga tgcaattggg gtgtcgccaa gcggtaaggc agcaggtttt gatcctgcca
    18601 tgcgttggtt cgaatccagc caccccagcc actttcccaa gcccccgcag cccaaagccc
    18661 ctcaaagccg tattcacaat tatttagttt tagggtggcc tcagtggcag acttaatggt
    18721 cttcagcggc aatgcgaatc ctccgcttgc gcaaaaggtt gttgaaaagc tcggcattcc
    18781 tttgggggac gccacctcat cgcagttctc cgacggcgaa atttccatcg aaattaacga
    18841 aaacgtgcgc ggtcgcgacg tcttcgtcat gcaatctacc tgcgttccta ccaacgacaa
    18901 tctcatggaa ttattgttta tggtggacgc cctgcgccgc gcttccgcag gccgcatcac
    18961 tgctgtatta ccctacttcg gctacgcccg ccaagaccgc cgcgtgcgct cagcccgggt
    19021 gcccatcagc gccaaagtag tggccgacat gatggccacc gcaggggtgg accgagtcct
    19081 caccgtggat ctgcacgccg aacaaatcca aggctttttt gatgtgccgg tggacaacgt
    19141 ttacggctca ccggtgttat tggccgatat agaactgcgc aaatttgaaa acctagtag
//
LOCUS       DAOQGS010000006        18686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1381_length_18686_cov_4.732704, whole genome shotgun sequence.
ACCESSION   DAOQGS010000006 DAOQGS010000000
VERSION     DAOQGS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_18686_cov_4.732704"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1112
                     /locus_tag="PKZ52_00510"
     CDS             123..1112
                     /locus_tag="PKZ52_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNG58272.1"
                     /translation="MSNIREVARLAGVSVATVSRAISNPEKVSPGSLKKVQQAIDAVD
                     YKPNLLARNFRAARSYCVVVLLPDITNPFFAQVIQSLEDRAQQKGYAVLLGDTRGSHK
                     REDDYISRVEMRLADGVIQLSPCSRNHTQRGGGNIAWVNACGVEGTPGPSVRVDNVAA
                     TRSMVEYLIALEHRKFGVVTGLQDNLHSRDRRQGIDLALGKAGIKLDPLWVQEGDFTL
                     RSGISAGEAFASMNKRPSAIICMSDQMAIGVIQALVSRGIRVPEDISVTGFDDIPYAH
                     CNNPTLTTLSQPAAAIGSTAMDLLLRVINGEDLTNTEIILPTELIIRQSSKAI"
     gene            complement(1277..1936)
                     /locus_tag="PKZ52_00515"
     CDS             complement(1277..1936)
                     /locus_tag="PKZ52_00515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG58273.1"
                     /translation="MSEPSMVSFSELELSFGYELFLIPDLNNKQQLFDCVLAGCIPEE
                     CLMVTAPASGMFPRVDCGQRVLVRIRLPSGVALFPTTILFISETPTLIVYLDYPRDIK
                     FKRVRAARVFVSLPVLMNSQSDEAVFGVAGKIVDVSTSGARIEATERLGNVGDIIEVK
                     GKFQVGNMVRMLSIHCIIRAEKTPKTYGVEFCEQNEEKLLVLLGFIYQSMAFNHAQAV
                     I"
     gene            2176..3729
                     /locus_tag="PKZ52_00520"
     CDS             2176..3729
                     /locus_tag="PKZ52_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNG58274.1"
                     /translation="MLELRNAAWGAPADQRWIFYPAVITIHAVRVPSPSMRLFPIVHL
                     CSVLLILYSVSMFGPILINVAYEDGDFNGFFTTFVLAFSIGITGWQLTRRGNTKLSSR
                     DGFLVVVLFWLVFSVVSALPFVLDNSVKASVADAFFESISGITTTGGTIMPHVDNLPH
                     AILFYRAQLNFLGGLGIIVLALAILPLLGVGGSKLYQSESVGPLREDKLSPRLADTAK
                     QLWMVYSGLALAAALSFKVAGLNWFDSFCYSLSTVALGGFATHDAGLGFYNNLWVEVV
                     AGVFSFLSGINFALYYRLTIARSVMPLFKDPELKAYALIALGVAAFTIIELWRCGRFP
                     MIECIVHGFFQSMSVMTDNGLTTADYPNWPAHIAIFLMLSSYIGGCVGSTCGGIKVMR
                     VLLCYRQTHQEIKQLIHTNGIFPTKINTHIVSDRMMQSVWSFAFLYIFISVVFVVALM
                     MCGNDIQTAFGTVSACINNMGIGYGDTAVNFAGLTDTAKWLMCAAMLLGRLEVFPILV
                     MFSRTYWRF"
     gene            3973..4692
                     /locus_tag="PKZ52_00525"
     CDS             3973..4692
                     /locus_tag="PKZ52_00525"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNG58275.1"
                     /translation="MPSQLHEFHIKEFISAAEQCFGATLVSLYLFGSAAEDKLRPQSD
                     VNVLVVLSQFCALAHTALQPALRQARAGIQLHVMFVETQELQQTADCFTAKFADIKAR
                     HRLVLGSDTLAAIDIKLPQLAHSAQQMLINFQLRSREQFLLAHSREEQLIKLIATAAA
                     PLRSSAAAVHLIRSGNWMDGKAALINLTAHNSAWSDAIHGLSTARETEALPEGCAKSY
                     FNALQEIARFLVTELHTHGGQ"
     gene            4692..5591
                     /locus_tag="PKZ52_00530"
     CDS             4692..5591
                     /locus_tag="PKZ52_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNG58276.1"
                     /translation="MFPFDLPGPQFLGFYSLFGIALVLLNTWQLRRQGVTSGADQAHT
                     FFNAPYALAYLNQGQQHCLSICAFNLIDRGLMTTNDAGQISSRSQDTKQVSHPLEHAL
                     IQYASSGAQKLFAAQRDNKVKVALSAIKADLLHRGLIAGGTFFTPFKLICLAFLAAVG
                     AIKITLALAHGHHNIQWLIVLMFIFMLVISLSRAPRLTADGEATLTIMRGLLAQLHGR
                     MQELKPGGFTQEATLAAALFGLAGLSLEYFPHLRSIMPPVVDSSGSSSDGGSSSGSSC
                     SSSCSSSDSSSSCGGGGCGGCGS"
     gene            5572..6771
                     /locus_tag="PKZ52_00535"
     CDS             5572..6771
                     /locus_tag="PKZ52_00535"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNG58277.1"
                     /translation="MAAVAAEDRFGLGWRGPLAAGILSSLEQLDCLEIIAEQYAHLPL
                     QQQRALQYLSKHIPITLHGVSLGLAGSTPPPPWRIENLARLSDELKAETVSEHLAFVR
                     AVAVKTDGTSNPHNSASIEIGHLAAPPRNGASIEQACEHIRAVQSLCARPLLVENIPS
                     LITPLGSDLTESQWLGQILQQSGAPFLLDLENLYANAVNRGENPIDHLLKLPLAQVHT
                     VHLSGGTWVDIPWQATTQRRFLDDHRNDILPEVFELLTRVAAQAPQNLTVIIERDGNY
                     PAMDKLLAQLAQAKLAVARGRQNAPTLIQRHASPLTTRSISTQSDNKRIEYLLAEIFC
                     NAKQRQLFVQNPYEFLQSLNLKHLEEDITNMDWAGLLLMARSVAAKNPTALAGRARSA
                     EITEHAF"
     gene            7404..9542
                     /locus_tag="PKZ52_00540"
     CDS             7404..9542
                     /locus_tag="PKZ52_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58278.1"
                     /translation="MSVNHMIIKPLALAVLSASLTGCFVPSEKITASSSSSSSSGSTK
                     PSSSSSSSSSSSSSSSSSSSGFATNPAVSITISENQDGLCSFDGVIATSVAGYRDAGY
                     LDFTNSASSKVTYRINAVDPGIYGLRVRYNHSSAQNLTGDVYVDGVKAGSFVGKAGSS
                     ANQWQLEDVAIQLKPGIHSIELRSTTSLGLPNIDSLEVIGDASPRACATSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSTGGVIPSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSGAVSSTPALKLVSPQAGANVGKGATAQVTVVVDL
                     TDEVYGGAVVGNATVTADGVTLAAESETRWKGVISVPKGGKSVEIVANVNGKTTKQTV
                     KYLNKSDPLKPYLLGINPGVYAMVFDPIATRIAKVGLNPTSGFAVWEDYLTNSQLKGE
                     NILFDFNSTNQHAYTVVNGNQLVAAGIAKSSPADYYGGALPSPINITFDSTNKRLLVL
                     SKASDKYSVYSVATASSSTADTPAFPNGKTSTTNSEAAATTFLRDLPADAVKGTYRHF
                     NFHRTSKTFVIADERSVGDSKVTVIQGYSEADGSKKFEVQVGADISNMAIANTEGMLY
                     VAENKSSLIASKLKAINVSTGALSDMGEIKGGITIADYSDLRIDNAKKKLYIGDAVGD
                     AIYEIDLNTKVLSELPLTAAQPQTGPVVDN"
     gene            complement(10100..11275)
                     /locus_tag="PKZ52_00545"
     CDS             complement(10100..11275)
                     /locus_tag="PKZ52_00545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNG58279.1"
                     /translation="MSVVKQGAIDASYMPALTGVRFFAIVHIFCFHLWVIFAMPKEEQ
                     FKTLLSDMAQLPKTLQTFLANGWMSTSFFFLLSGFILAFIYWAPDGQLAMPKKKFWLL
                     RITRIYPIHILLVLLTIALTAGYQISQGASVPLLISSAIATLTLTQAWNPNWVPVWSW
                     PTWTVSALVFLYALMPYLMPRLAVLSRRSMVALLAALPFISLIPTCIYALYFPVGSKP
                     EQFWQIFVGSTPVFWLAHFVAGMLLTRIFGLSRFNTLGISSAKKNLAWGDGAFLAVIA
                     IGCIPDIAEPYKYFLRHGLMMPLYMLIIVDLARGNGLIARLLSFRVMSFLGETGFSIF
                     IWQNMVMIICWIAVMANPALGAHQFKWALVGIVLLGVVSTYAFEKPFSKWLRQRLRH"
     gene            complement(11437..12210)
                     /gene="hisF"
                     /locus_tag="PKZ52_00550"
     CDS             complement(11437..12210)
                     /gene="hisF"
                     /locus_tag="PKZ52_00550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNG58280.1"
                     /translation="MALAKRIIPCLDVDKGRVVKGVQFLDIRDAGDPVEIAKKYNEQG
                     ADEITFLDITASHEARDTTVHTVEAIAAEVFIPLTVGGGIRTLDDIRRMLNAGADKVS
                     INSAAVFNPPFVQEAAEKFGSQCIVVAIDCKKVSGPGELDRWEIFTHGGRKPTGLDAV
                     EWAVRMTEFGAGEILLTSMDQDGMKRGFDLGITRAIADAVAVPVIASGGVGNLQHLVD
                     GIVQGGADAVLAASIFHFGEYTVPQAKAFMKAQGIEVRD"
     gene            complement(12231..12965)
                     /gene="hisA"
                     /locus_tag="PKZ52_00555"
     CDS             complement(12231..12965)
                     /gene="hisA"
                     /locus_tag="PKZ52_00555"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNG58281.1"
                     /translation="MLIIPAIDLKDGQCVRLRQGLMDDSTVFSNDPVAMAARWVAEGA
                     RRLHLVDLNGAFEGAPVNGDVVTQIARAYPNLPIQIGGGIRSLATIEHYLAAGVQYVI
                     LGTKAVKEPAFVKEACSAFAGHIIVGLDAKKGLVATDGWAEVSSVKATDLAKQFESDG
                     VSAIVYTDIDRDGMMQGVNIEATVQMAQASSLPIIASGGVTNLGDIEQLLRVASHGIC
                     GAITGRAIYEGTLDLAAAQRYCDQNA"
     gene            complement(13020..13682)
                     /gene="hisH"
                     /locus_tag="PKZ52_00560"
     CDS             complement(13020..13682)
                     /gene="hisH"
                     /locus_tag="PKZ52_00560"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNG58282.1"
                     /translation="MAQVAIIDYGMGNLHSVASALHLVSPASEIVITADPAVIANAER
                     IIFPGVGAMRDCMGEIKRLHLDACLQQALDAGKPTLAICVGMQALLASSEENGGVPCL
                     NMLPGAVYGFNQDSRFKQINDTLQGPQRLKVPHMGWNQVVHTQSHPLWQGIADNSRFY
                     FVHSYFVQTSDESLVAGRATHGIPVVAALARRNLFAVQFHPEKSHQAGLTLLNNFMHW
                     QP"
     gene            complement(13682..14290)
                     /gene="hisB"
                     /locus_tag="PKZ52_00565"
     CDS             complement(13682..14290)
                     /gene="hisB"
                     /locus_tag="PKZ52_00565"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNG58283.1"
                     /translation="MTEQAKSARIACVNRNTLETQIQVALNLDGTGKGEFATGVPFLE
                     HMMDQIARHGMIDLKVDCRGDTHIDDHHSVEDIGITIGQAISQAVGDKRGIRRYGHAY
                     VPLDEALSRVVIDFSGRPGLIMHIPFTQKRIGQFDTELFWEFFQGFVNHAGVTLHIDS
                     LRGHNAHHQIETVFKAFGRALRMALEWDERLGNAMPSTKGSL"
     gene            complement(14359..15270)
                     /locus_tag="PKZ52_00570"
     CDS             complement(14359..15270)
                     /locus_tag="PKZ52_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNG58284.1"
                     /translation="MANSLLFAGIEAGGTKINCVVGWAGGDIVHQQQFPTDKPSAVLP
                     HLQSYFLTQSHKYGAIAALGIGSFGPLQLDATAADYGCIWATPKAGWGGFNWLRFWRD
                     ALRVPVALQTDVNAALIAEQALGHARGLRHAVYVTVGTGIGGGIMLDGKILQGASHPE
                     LGHMAVKRRADDIFAGVCPFHGDCLEGVASGPALSKRWMMPAQQLPADHPAWDLQAFY
                     LAQLCVNVTLCYGPERIVIGGGVAAQGQMMALLRKEFVRQINGYCRPELITAVEAYIQ
                     RSGLGGNAGQIGSLLLAEAIYREQSGL"
     gene            complement(15318..15533)
                     /locus_tag="PKZ52_00575"
     CDS             complement(15318..15533)
                     /locus_tag="PKZ52_00575"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNG58285.1"
                     /translation="MTPFKPPPINASVASPCVRQCCLNAQDMCVGCGRYLAEITGWLN
                     FTAAEKMTVVHNAQQRLKDAANKKPPG"
     gene            complement(15530..16240)
                     /locus_tag="PKZ52_00580"
     CDS             complement(15530..16240)
                     /locus_tag="PKZ52_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58286.1"
                     /translation="MFKKLHLTKITDTLDILQARISERFADCGLLNVCVELNAIARAS
                     EARITWITRPNRSIRVGVACVIMGGVMSGLMGFHWVKLQPSVPSFGEFVQLTEAFLNT
                     LVLVGASLFFLVSLESRIKRSRTLKALHELRAIAHVIDMHQLTKDPVISYSVGEQPTP
                     SSPKRQLNAFELRRYLDYCAEMLSLVGKTAALYSQQMPDAEIVSAANDVEILCASMSH
                     KIWQKIMIIHADQQRRAL"
     gene            16957..18324
                     /gene="pcnB"
                     /locus_tag="PKZ52_00585"
     CDS             16957..18324
                     /gene="pcnB"
                     /locus_tag="PKZ52_00585"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNG58287.1"
                     /translation="MLNRIIKFITGKKGSKGPAQYSVDQLQIDLNRVSRNAIKVVDML
                     QSAGYSAYIVGGGVRDLLLNLKPKDFDVATNATPEQVRQVFRSALIIGRRFRIVHVRF
                     GREIIEVTTFRAHHQESSNAKDAKQSDHGLLLLDNVYGDLESDAFRRDFTVNALYLDP
                     NTRQIIDFAEGIEDLKQRQLRIIGEAEARYREDPVRMLRAVRFAAKLGFRLEASSERP
                     ISALAPLLAHIPPARMFEEVLKLFLSGSATACFHLLRQYDLFYYLFPGTEEVLKTQDP
                     ISEAIAERVVINTDKRIRSDLRVTPAFIYAVFLWLPLQHAVKKLQSQGVSPQDALQKA
                     GSDVIAKQLSRTAIPKRFLIPLREIWALQWRLPKRDGGKAFGLLEHPRFRAAYDFLLL
                     REEAGENLDGLGHWWTQFQNADSASREEMVKGLESPGKRKTRKRRPKKRGSGASPEAA
                     QDDTE"
     gene            18321..>18686
                     /gene="folK"
                     /locus_tag="PKZ52_00590"
     CDS             18321..>18686
                     /gene="folK"
                     /locus_tag="PKZ52_00590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNG58288.1"
                     /translation="MSFTRVYIGLGSNLQAPIAQLTQACKALKQLAPADDFLCSPFYQ
                     SKAVGPAQPDYINACATFSTELAPLDLLDALQAIEAQQGRTRSLHWGPRTLDLDLLLY
                     GSLCLDNPRLQLPHPYLKER"
BASE COUNT         4863 a         5106 c         4356 g         4361 t
ORIGIN      
        1 aggctacttg tgcgcagaat aacgggcagc atcgcaatgt aaccgtttgc gccctcagta
       61 tacacacgcc gtcgtcgtgt atcctttcgg ctgattaatc tgtaaaaatt actgagttcc
      121 ccatgtccaa tattcgagaa gtagcccggt tggccggtgt atcagttgca actgtttcac
      181 gcgcgatcag taacccggag aaagtctctc ccggtagctt aaaaaaagta caacaagcca
      241 tagacgcagt ggactacaaa cccaacctat tggcccgcaa cttccgcgcg gcgcgctcct
      301 attgtgtagt ggtgctgctg ccggacatca ccaatccctt ttttgctcaa gtaattcaaa
      361 gccttgaaga ccgcgcccag caaaaaggtt atgcggtgtt actcggcgac acccgtggct
      421 cccacaagcg tgaagacgat tacatcagcc gcgtggaaat gcgcctggcc gatggtgtga
      481 tacaactgag cccctgctcc cgcaaccaca cccagcgggg cggcggcaat atcgcctggg
      541 taaatgcctg tggcgtggag ggcacaccgg ggccatcggt gcgggtggac aatgtggccg
      601 ccacccgctc catggtggaa tacctcatcg ccctcgagca ccgcaaattt ggcgtggtca
      661 ccggcctgca agacaaccta cacagccggg atcggcggca ggggatcgac ctggcattag
      721 gtaaagcggg cataaaactc gacccgttgt gggtgcaaga aggcgacttt accctgcgct
      781 ccggtatcag cgcaggcgag gcctttgcca gtatgaataa acgccccagt gcaattattt
      841 gcatgagcga tcagatggcc atcggcgtca ttcaagccct ggtttcccgt ggcattcggg
      901 tgccggagga tatttcggtg accggttttg acgatattcc ctacgcccac tgcaacaacc
      961 ccaccctcac cacgctctcc caacccgccg cagccatagg ctcaacggcc atggatctgt
     1021 tgctgcgggt aattaatggg gaggatttaa ccaataccga aattattctg cctacggaat
     1081 taattattcg ccaaagcagc aaggcgattt aaacggcagg ttattaaccc ggcggcattt
     1141 agtgccgggt taatagtggc gaaggttggg caggaaacct gggacttaag ccacagggat
     1201 gcgcctatgg atttttaaaa tagtcaccga ttcaaactga gcaggcgtat ttaattttaa
     1261 atcgactcac atctacttaa atcactgcct gggcgtgatt aaaggccatg gactggtaaa
     1321 taaaccccaa tagcaccaat aacttttcct cgttctgctc gcaaaattcg acgccgtagg
     1381 ttttcggggt tttttccgcg cgaatgatgc aatgaatcga caacatccgc accatattgc
     1441 ccacctgaaa cttccccttc acctcaataa tatcccccac attgcccaat cgctcagtgg
     1501 cttcaatgcg ggcgccggag gtgctgacat caacaatttt acccgccaca ccaaaaactg
     1561 cctcatcgct ttggctgttc atcaataccg gtagcgacac aaacacccga gcggcgcgca
     1621 cccgtttaaa tttgatatcg cggggataat ccaaatagac aatcagcgtc ggcgtttcgg
     1681 aaatgaataa aatagtggta ggaaaaagcg ccacgccgct gggcaggcgt atgcgcacca
     1741 acacccgctg gccgcaatcc actctgggaa acataccgga ggcaggcgcc gttaccatca
     1801 aacactcttc cggaatacag cctgctaaaa cgcaatcaaa caactgctgc ttgttgttta
     1861 ggtctggaat tagaaacagc tcatatccaa agctgagctc taactcgcta aaactcacca
     1921 ttgacggctc actcatgcag cacacctacc tcgtaacaca tctctaaggt tagaccacat
     1981 atgccgtgca cgccgccagc aacaccgata aaagaacaga tcaactaagc cccctgcaaa
     2041 ccgccgcaaa cccgatactc gacagtcttt atacagctga atgaacgagc ggcctgcgca
     2101 ccattgccac gccccagcgc ttgcgaacac atctctcacc tgggtttttg aagaacacgc
     2161 atcagcctaa aaactgtgct tgaacttagg aacgctgcct ggggggcgcc tgcggatcag
     2221 cgctggattt tctaccccgc tgtaattacc attcacgccg tccgagtgcc gagcccctcc
     2281 atgcgccttt tcccgattgt ccatctgtgc agcgtgctgt taatcctcta tagcgtgtct
     2341 atgttcggcc cgatcctcat caacgtcgcc tacgaggacg gcgacttcaa cggttttttc
     2401 accacctttg tactcgcgtt tagtatcggc attaccggct ggcaactcac ccgtcggggc
     2461 aacactaagc tctcctcgcg ggatggtttt ttagtggtag tgttattttg gctggtattt
     2521 tccgtggtca gtgcgctgcc atttgtattg gataattccg tcaaagccag tgtagcggac
     2581 gcgtttttcg agagtatttc cggcatcacc accacaggcg gcaccatcat gccccacgtg
     2641 gataacctgc cccacgccat tttattttat cgcgcgcaat taaatttctt gggcggcttg
     2701 ggaattatcg tgttggcctt ggccatattg ccgctgcttg gggtgggcgg ctccaagctc
     2761 tatcaatccg aatccgtagg cccattgcgg gaagataaac tcagcccccg cttggccgac
     2821 accgccaaac aactctggat ggtgtactca ggcctcgccc ttgcggccgc actgtcattt
     2881 aaagtggccg gtttaaattg gtttgactcc ttttgttact cgctctccac cgtggccctg
     2941 ggcggctttg ccacccatga tgcgggctta ggtttttaca ataatttatg ggtggaagtt
     3001 gtcgcgggcg tattttcgtt tttatcgggt atcaactttg cgctctatta ccgcctgacg
     3061 attgcacgca gtgtgatgcc gctttttaaa gatcctgaat taaaagccta tgcgcttatc
     3121 gcgctggggg ttgctgcatt tactattatc gagctttggc gctgcggccg ttttccaatg
     3181 atcgaatgca tcgtgcatgg gttttttcag tccatgtcgg tcatgactga taacggcctg
     3241 accaccgcag actaccccaa ttggcctgcg cacatcgcca tatttttaat gttgtccagc
     3301 tacatcggcg gctgcgtggg ctccacctgt ggcggcatta aagtcatgcg ggtactcttg
     3361 tgttatcggc aaactcacca agaaattaaa cagctgattc acaccaatgg tattttcccc
     3421 accaaaatta atacccacat cgtctccgac cgcatgatgc aatccgtctg gtcatttgca
     3481 tttttgtata tttttatcag cgtagtcttt gtagtggcgc tgatgatgtg cggcaatgat
     3541 attcaaaccg ccttcgggac tgtatcggcc tgcattaata atatgggcat tggctatggc
     3601 gataccgccg tgaattttgc aggcttaacc gacaccgcca aatggctgat gtgtgccgcc
     3661 atgttattag ggcggctgga agtattccct attttggtta tgttctcgcg cacctattgg
     3721 cgcttttaac ctaaaagccg cagttgaact cgtagcgagg gtgcctaagg cgctgaatat
     3781 gagcgccttt tttgtcaaaa tttggcgaaa gcagtagtca ccactagggt gagtcaaatt
     3841 ttgacaaaaa agcgtgcaga ttcaagcatt aggcggccat agtagagttg aactacggtt
     3901 tttaggttta aatattcact ctaatttttc gcagttaaca attgtactgt tcacccgcca
     3961 gaaggattta ttatgccctc ccagctacac gaatttcaca tcaaagaatt tatcagcgcc
     4021 gccgaacagt gttttggcgc gaccttagta agcctctatt tatttggctc cgccgccgaa
     4081 gacaagctgc gcccccaatc cgatgtgaat gtgctcgtgg tgctcagcca attttgcgcc
     4141 ctggcacaca cggctttgca acctgcctta cggcaggccc gcgcggggat tcaactgcac
     4201 gttatgtttg ttgaaacaca ggaattacaa cagaccgccg actgttttac ggcaaaattc
     4261 gccgatatta aagcgcgcca ccgccttgtg cttggcagtg acacccttgc cgccatcgac
     4321 ataaaactgc cgcagttggc gcactccgcc caacaaatgc tgattaattt tcaactgcgc
     4381 agccgcgagc aatttttatt ggcccacagc cgcgaagagc aactgattaa actcatcgcg
     4441 acagcagccg caccactgcg ctccagcgcc gccgcggtgc acttaattcg cagtggcaat
     4501 tggatggacg gcaaggcggc actcattaac ctcaccgccc ataattccgc ctggagtgat
     4561 gccatccacg gcctatctac cgcacgggaa accgaggctt taccggaagg ctgcgcgaaa
     4621 agttatttta atgcgctgca ggaaattgcg cgctttttag tgaccgaatt gcatactcac
     4681 ggaggccaat aatgtttcca tttgacctcc ccggcccgca atttctaggc ttttatagtt
     4741 tattcggcat tgccctggtg ctattaaata catggcaatt acgccgccaa ggtgttactt
     4801 cgggcgccga ccaggcccat acatttttta atgcgcccta cgctttagcc tatcttaatc
     4861 aggggcagca gcactgcctg agcatttgcg cctttaattt aattgatcgc ggcttaatga
     4921 cgacaaatga cgcaggccag attagcagcc gctctcaaga caccaagcag gtatcccacc
     4981 cgcttgaaca cgcactgatt caatacgcat cttcaggtgc ccaaaagctt tttgccgccc
     5041 agcgcgataa caaagtaaaa gtggcgctca gcgccattaa ggcagattta ctgcaccgtg
     5101 gccttattgc cggcggcaca ttttttacgc cgtttaaatt aatctgcttg gcttttttag
     5161 ctgccgtagg ggctataaaa atcacacttg ccctggccca tgggcatcac aatattcagt
     5221 ggctgattgt tttgatgttt atttttatgc tggtgatcag cttaagccgc gcgcctaggc
     5281 ttaccgctga tggtgaagcg accctgacaa taatgcgcgg cttattagcg caattacacg
     5341 gccgcatgca ggaattaaaa cccggcggct ttacccaaga agccactcta gccgctgcac
     5401 tatttggctt ggcaggtctc agcctcgagt actttcccca tctgcgctcg attatgcccc
     5461 ccgtagtcga ttcatcaggg tcatccagtg acggcggcag cagcagtggc agctcctgca
     5521 gtagcagctg cagcagttca gacagctcga gcagttgtgg gggcggtggt tgtggcggct
     5581 gtggcagctg aggatcgctt cggcttgggc tggcggggcc cgctcgcagc gggaattctc
     5641 agttcgttag agcaattgga ttgcctagaa atcatagccg agcaatatgc ccacctgccg
     5701 ctccaacaac agcgcgccct gcagtattta agcaaacaca ttcccatcac cctgcacggc
     5761 gtgagtttag gcctggctgg gagcacaccg ccgcccccat ggcgcattga aaacctcgcg
     5821 cgcctgagcg atgaactaaa ggccgaaact gtctccgaac atttagcttt tgtgcgtgca
     5881 gtggccgtaa aaaccgacgg cacatccaac ccacacaaca gcgcgtcaat tgaaattggc
     5941 catttagccg ccccaccgcg caacggcgcc agtatcgagc aggcttgcga gcatattcgc
     6001 gcagttcaga gcctctgcgc gcggccatta ctcgtggaaa atattcccag cctaattacg
     6061 ccactgggca gtgatttaac cgagagccaa tggctcgggc agattctgca acagtccggc
     6121 gcaccatttt tattggattt agaaaacctc tacgccaatg cggtaaatcg cggtgaaaac
     6181 ccgattgatc acctacttaa gttgccgctg gcacaggttc acaccgtgca tttaagcggc
     6241 ggcacctggg tggatattcc ctggcaggct accacccagc ggcgcttttt ggatgatcac
     6301 cgcaacgata ttctgccgga agtttttgag ctcttaacca gggtagcggc acaggcgcca
     6361 cagaacctta cggtcattat cgagcgcgat ggcaactacc ctgcgatgga taagctgttg
     6421 gcacaattag ctcaagccaa actcgccgtc gcacgcggcc gccaaaacgc acccacatta
     6481 attcaacgcc atgcatcgcc attaacaacg cggtcgataa gcacacagag cgataacaaa
     6541 cggattgagt acttactcgc ggaaattttt tgcaacgcaa aacagcggca attattcgta
     6601 cagaatcctt atgaatttct tcaatcgctc aaccttaagc atttagaaga agatataacg
     6661 aatatggatt gggccggttt attgttaatg gcgcgcagtg tcgccgcgaa aaatcccacc
     6721 gccctagctg ggcgcgcaag atcagcggaa ataactgagc atgcgtttta atggctattg
     6781 ccaaaatcgc agaaaccccc tgcaattagc agcgtagaaa cgtcttaata tttttattaa
     6841 aaccacccat ttaccaggcc ctgcgcagca ccctattggc gattaatgtt cgccaggcat
     6901 cgctggccgc ctcagatttg tgagtgaagg ttcaccacgg gttttaacag cggtttttac
     6961 actccaaaaa tcctcattga aatggtttga cggctgataa aaggcgcgct gagaaaaata
     7021 atcacctcat acacaagctg ccccagagat ttctcaccca atttaatcca ctgacaaatc
     7081 cgctcctgct ccctgggtct tcctcagaga gattgcccca taattggggg aaatacgagc
     7141 agatgtaata aatgtgaact gggccgcatg aataatgctg attaatcacc gctcaatcct
     7201 tcagctatca aactaggtct ctttttaagg attcggctta ggatagaata ggcctaagag
     7261 caaaaagcca ggttaagtct gataaaccta gattttactt gtaaggcctg atctggatgt
     7321 cggccgcggc ttagtgccaa tacttttcgt acatggtgtt gggttgattc cggtaccgat
     7381 aataacaact gcttggagga cctatgtccg ttaaccacat gattatcaaa cctctggcgc
     7441 ttgccgttct atctgcatcg ctgacgggct gttttgtacc atccgaaaaa attactgctt
     7501 cctcttcaag ctcctccagc tcaggctcca ccaagcccag ctcatcttcc tcctctagct
     7561 cgtcttcaag ctcatccagc tcgtcttcaa gctcgggttt tgccactaat cctgcagtat
     7621 ctatcaccat ctcagagaac caagacggct tgtgttcatt tgatggcgta atcgctacct
     7681 ccgtcgcagg ctatcgcgat gcagggtatt tggatttcac caacagtgcg tccagcaaag
     7741 ttacctatcg tatcaatgcc gtggacccgg gcatttatgg cttgcgtgtt cgctacaacc
     7801 acagcagtgc gcagaacttg actggggacg tttatgttga tggcgtaaaa gcaggcagct
     7861 tcgtaggcaa agctggcagt tcagccaatc aatggcaact tgaagatgta gccattcagc
     7921 tgaaaccagg gattcacagc atcgagctgc gttcaaccac atccttagga ttacctaata
     7981 tcgactcact tgaggtcatt ggtgacgcat cgccccgcgc ctgtgcaact agctcatcaa
     8041 gctcaagctc tagttcgagc tctagctcct cgtcaagctc aagttcttct tcaagttcaa
     8101 gctcatcttc aagttctagc agctctactg gcggcgtaat tccaagcagc tcgtcaagtt
     8161 catctagctc aagcagttct tctagttcta gctcaagcag ttcttctagt tcaagctcaa
     8221 gcagttcttc tagttctagc tcaagcagtt cttctagctc aagctcaagc agctcttcta
     8281 gttcaagctc aagcagctcg tcaggcgcag tctcaagcac cccggcatta aaactggttt
     8341 caccacaagc tggagccaac gtaggtaagg gtgctaccgc ccaagtcact gtggtagttg
     8401 acctgacaga cgaagtatac ggtggcgcag tggtgggcaa tgccaccgtt accgctgacg
     8461 gagtaacctt ggctgctgag agcgaaaccc gctggaaagg tgttattagc gttcctaaag
     8521 gtggtaagag cgttgagatt gttgccaatg tgaacggcaa aaccactaag caaaccgtta
     8581 aatacctcaa caaatcggat ccgcttaagc cttacctctt gggtattaac ccaggtgtat
     8641 acgcaatggt atttgaccca atcgcgaccc gcatcgctaa agtcggttta aacccaactt
     8701 ctggttttgc cgtttgggaa gactacttga ccaactccca gttgaagggc gaaaacattc
     8761 tgttcgactt taactcgact aaccaacacg cttacacagt ggttaatggc aatcagctgg
     8821 tggctgcagg catagccaaa tcaagccctg ccgactacta cggcggcgca ttgcccagcc
     8881 ccatcaatat tacctttgac tccaccaata agcggctgtt agttctatcc aaagcgtctg
     8941 ataaatacag cgtttactca gtagcgaccg catcgtcaag cacagctgat acaccagcgt
     9001 tcccgaacgg caagacttcc acaaccaata gcgaagctgc agcaacaacc tttttaaggg
     9061 acttgccagc agacgcagtt aaaggcacat accgacactt taacttccac cgaaccagca
     9121 aaaccttcgt aattgccgac gaacgctcgg taggtgattc taaggtaaca gtaattcaag
     9181 gctactctga agcagacggc tcgaagaaat ttgaagtcca ggttggagcc gatatttcca
     9241 atatggctat cgccaatact gaaggcatgc tttatgttgc agagaataaa agttctctga
     9301 tagcgagcaa gctgaaggct attaacgtga gcacgggtgc tctaagtgat atgggagaaa
     9361 tcaaaggtgg tatcaccatc gcagattact cagatctacg catcgataac gctaagaaaa
     9421 agctctacat tggtgatgcg gtgggcgatg ctatttacga gatcgatcta aacactaaag
     9481 tgcttagtga gcttccattg actgccgctc aaccacaaac tgggccagta gttgataact
     9541 aatttgagtt agtgggaatt aaagccggtg gaaacaccgg cttttttttg gtcccaatat
     9601 tggcgcaata cgaacttatt agactccgca agcaaactaa caacaaacgg aaaaccccct
     9661 tacaagcata aaacagcaag cattcttaat gaactaagcg cgaagttgct tgtacacgga
     9721 caactccaat acatgcacct agttattggc ccattatgac cttgggcact ttaatcagga
     9781 ttgaatcgta tatattttaa atatttacta gcatgctccg gcggtaagta cagaggcata
     9841 cctgcccgca tagctatcaa aaagcaacaa atctgtgtaa ttctaatcaa ccggaagata
     9901 tagacctaaa ctgccaatca atgaattgac gcacccgcca ctagcaacac agctaaaata
     9961 ttggaaattc acctagggct aaatactatt cactccaccg atcatcctgc agcaagagat
    10021 cttgagcaat cacttccact gcatattact ttggcgagct caggaataaa gcccgcagca
    10081 cttttcatcc aataatggat cagtgcctta agcgctgccg caaccattta gaaaatggct
    10141 tttcaaaagc ataagtactt acaaccccca acaacactat ccccaccaaa gcccacttaa
    10201 attgatgggc gccaagggct gggttcgcca taacggcaat ccagcaaata atcatcacca
    10261 tattttgcca aataaaaatc gaaaaaccgg tttcacctaa aaaactcatt acgcgaaacg
    10321 ataataggcg tgcgatcagc ccattcccgc gggcaaggtc aacaataatc agcatataaa
    10381 gcggcatcat caggccgtgg cgtaaaaaat atttataggg ctcagcaata tccggtatac
    10441 agccaatagc tattaccgct agaaacgccc catcacccca ggcaaggttt ttctttgcag
    10501 atgaaatgcc tagggtatta aacctcgaca aaccaaaaat gcgtgtaagc aacatgcccg
    10561 ccacaaaatg cgccaaccaa aatacagggg tagagcccac aaaaatctgc caaaactgct
    10621 caggtttaga cccgacagga aaatacagcg catagataca ggtaggtatt agagaaataa
    10681 acggcagcgc cgccaataaa gccaccatgg agcgccgcga taaaaccgcc agcctaggca
    10741 ttaaatacgg cataagcgcg tacaaaaata ccagggcgga aacagtccaa gttggccagc
    10801 tccacacggg aacccaattg ggattccagg cttgtgtcaa tgtaagggtg gcgatggcac
    10861 tggaaattaa cagcggtaca ctcgctccct gggatatttg gtagccagcg gttaaggcaa
    10921 tggttaacaa cactaacagt atgtgaatcg ggtaaatacg cgttatgcgc agcaaccaaa
    10981 atttcttttt gggcatagca agttgtccat ccggcgccca ataaataaaa gccagaataa
    11041 accccgataa caaaaagaaa aaactggtag acatccagcc attagctaaa aatgtctgca
    11101 gtgttttagg taactgcgcc atatcgctga gcaaggtttt aaactgttcc tctttcggca
    11161 tggcaaatat cacccagagg tgaaaacaga aaatatgcac aatggcgaaa aatctcaccc
    11221 ccgtgagcgc aggcatatag gaggcatcaa tcgctccttg tttgactacc gacataaaca
    11281 attcccctta tgttttaatc aatctaacac ttttatagca actgactgag caaatggtaa
    11341 ccataaacaa taaaaaactc cctaattagt cacctgtagc tcaaataaat aacccagccg
    11401 aagctgggtt atttattaaa aaacttcgat cctgtgttag tccctaactt caataccctg
    11461 ggccttcata aaggctttgg cctgcggcac tgtatattca ccaaagtgaa aaatactcgc
    11521 agccaatacg gcatcggcgc cgccttgcac tatgccatcc actaaatgct gcaaattacc
    11581 cacgccgccc gaggcaataa caggaaccgc caccgcatcg gcaatagcgc gggtaatgcc
    11641 caaatcaaag ccgcgtttca taccgtcctg atccatgctg gttaataaaa tttccccagc
    11701 accaaattct gtcatacgca ctgcccactc aaccgcatcc aaaccggtgg gcttgcggcc
    11761 gccgtgggta aaaatttccc agcggtctaa ctcacctggc ccgctgactt ttttgcagtc
    11821 tatggccact acaatacatt gagagccaaa tttttcggca gcttcttgca caaatggcgg
    11881 gttaaatacg gcggcggagt taatggatac cttatcggcc cctgcattta acatgcgccg
    11941 aatatcatcc aaagtgcgga taccgccgcc gacggtgagc ggaataaaca cttcagcggc
    12001 aatggcttcc acggtatgca cggtggtatc gcgggcctcg tggctggcgg taatatccaa
    12061 aaaggttatt tcatccgccc cctgttcatt atatttttta gcaatttcaa caggatcacc
    12121 cgcatcgcga atatccaaaa attgtacgcc ttttacgacg cggcccttat ctacatccaa
    12181 acagggaata atgcgtttcg ccagtgccat aggaaattct gcagtaaaat ttaagcgttt
    12241 tggtcgcagt aacgttgtgc ggcggccaaa tccaaagtgc cttcataaat agcgcggcca
    12301 gtaatggcgc cgcaaattcc gtgggatgct acacgcaata attgttcgat atcgccaagg
    12361 ttagttacgc cgccactggc aataatcggc aaactagaag cctgcgccat ttgcacagtc
    12421 gcctcgatat taaccccctg catcatgccg tcgcggtcga tatccgtata cacaattgca
    12481 ctaacgccat cgctttcaaa ttgcttggca agatccgtag ctttaacgct ggatacttct
    12541 gcccaaccat cggtggccac caaacctttt tttgcatcca agccaacaat tatgtggccc
    12601 gcaaatgcac tacaggcttc tttgacaaac gcaggttctt ttaccgcttt agtgccgaga
    12661 atcacgtact gcactcccgc cgccaaataa tgctcaatgg tggccaaact gcggatgccg
    12721 ccgccgattt gtataggtaa atttggataa gcgcgggcga tttgtgtgac tacatcgccg
    12781 ttgacgggcg cgccttcaaa agcgccattt aaatccacca gatgcaaacg gcgcgcaccc
    12841 tcggccaccc agcgggccgc catagcgacc ggatcgttag aaaatacagt ggagtcatcc
    12901 attaagccct ggcgcaagcg cacgcactgg ccgtctttta aatctatggc tggaataatt
    12961 aacatgggat actcaaataa aaataaaaaa gatgcgcagg cactagttcg cggtgcgaat
    13021 tacggctgcc agtgcataaa attgtttaac aaggtcaagc ccgcttggtg gcttttttcc
    13081 gggtggaatt gcacggcaaa caaattgcgc cgtgccaatg cggccaccac aggaatacca
    13141 tgggtggccc taccggcgac gagcgattca tcagatgttt gcacaaaata actgtgcaca
    13201 aaataaaaac ggctgttatc ggcaataccc tgccaaaggg ggtgagactg agtgtgcact
    13261 acctgattcc agcccatatg cggcactttt aatcgctgcg gcccctgcaa agtgtcgttt
    13321 atctgtttaa aacgggaatc ctggttaaaa ccgtatacag cgcctggcag catatttaaa
    13381 cagggcacac caccattttc ttccgatgag gccaacagcg cttgcatgcc cacgcaaatg
    13441 gctaaagtcg gtttacctgc atccagcgct tgttgcaggc aagcatctaa atgaagccgt
    13501 tttatttccc ccatacaatc gcgcatagcg ccgacgcccg gaaaaataat ccgctcggca
    13561 ttggcgataa cggcaggatc agccgtaata acaatttcac tggcggggct tactaaatgc
    13621 aaagcactgg cgacagagtg caaattgccc atgccgtaat caattattgc tacctgcgcc
    13681 attacagaga tccttttgta ctgggcatgg cattacctaa gcgttcatcc cactccagag
    13741 ccatgcgcaa agcgcggcca aaggctttaa atactgtttc aatttgatga tgcgcgttgt
    13801 ggccacgcag actatcaata tgcagtgtga cgccggcgtg gtttacaaag ccctgaaaaa
    13861 attcccaaaa taattcggtg tcgaattggc caatgcgttt ttgggtaaaa ggaatgtgca
    13921 tgattaaacc gggccgaccg gagaaatcga tcactacccg ggataaagcc tcatcaagcg
    13981 gtacataggc atgtccgtag cggcggatac cgcgtttatc ccccaccgcc tggctgatcg
    14041 cctggccgat ggttatgcct atatcttcca cactgtggtg gtcatcaata tgggtatcac
    14101 cccggcagtc cactttaaga tcaatcatgc cgtgacgagc gatttgatcc atcatgtgct
    14161 caagaaaagg cacacccgtt gcgaattcac ctttgccggt gccatcgaga ttgagagcca
    14221 cctggatttg ggtttccaaa gtgttacggt tgacgcaggc aatgcgcgcg gatttagctt
    14281 gctcggtcat agatgtgccg ccagtcaaaa gccggggatt atagccactc tggctgctgt
    14341 gctcaattta acccgcagct aaagcccgct ctgctcccgg tagattgcct cggccaacaa
    14401 taaactgccg atttgcccgg cattgccgcc taggccactg cgctgaatat aggcctccac
    14461 tgccgtgatc aattcaggcc ggcaataacc attaatttgc cgcacaaact ccttgcgcag
    14521 cagcgccatc atttgcccct gggcggcaac gccgccacct atcacaatgc gctcagggcc
    14581 ataacagagt gtgacgttga cgcacagctg cgccaaataa aatgcttgta aatcccaagc
    14641 gggatgatcg gcaggcaact gctgggcggg catcatccag cgtttgctca gcgcaggtcc
    14701 cgaggcgacc ccctctaaac aatcgccatg gaagggacac acaccggcaa agatatcatc
    14761 ggcgcgccgt ttcaccgcca tatggccaag ctccgggtgg cttgccccct gcagaatctt
    14821 cccatccagc ataatgccgc cacctatgcc ggtgcccaca gtgacataca ccgcatggcg
    14881 caaaccccgc gcgtggccca gggcctgctc agcgatcaag gcggcgttga catcggtttg
    14941 cagcgccact ggcacccgca gggcatcgcg ccaaaacctt agccaattaa acccacccca
    15001 gcccgcttta ggtgtagccc aaatacagcc gtaatccgca gccgttgcat ctaattgcag
    15061 ggggccaaaa ctgcctatcc ccagcgccgc aatggcaccg tatttatggc tttgcgtcaa
    15121 aaaataactc tgcaggtgcg gcaatacggc gctgggctta tcggtgggaa attgttgttg
    15181 gtggacaata tcgccccctg cccaaccgac cacacaatta atttttgtac cccctgcttc
    15241 tatcccagca aacagcaaag aattagccat taaatcactc cagaattcgg catctaaatt
    15301 attcgataaa tattgtatta accaggcggc tttttattgg ctgcatcttt tagccgctgc
    15361 tgtgcattgt gaacaactgt cattttctct gcagcggtga aattcaacca accggtaatt
    15421 tccgccaaat accgcccgca gcctacgcac atatcctgcg catttaaaca acactggcga
    15481 acacaggggc tagctacaga agcgttaatt ggcggaggct taaaaggagt catagggcgc
    15541 gccgctgctg atccgcgtgg ataatcataa ttttttgcca aattttatgg ctcatgctcg
    15601 cacagagtat ttccacatca ttggcagcgg agacaatttc agcatccggc atttgctggg
    15661 aataaagtgc cgcagttttg cccacaaggg ataacatttc cgcgcagtaa tcgaggtaac
    15721 ggcgcaattc aaaggcattt aattgccgct taggcgaaga tggtgtgggt tgctcgccaa
    15781 cgctgtaact gataacagga tccttagtga gctgatgcat atcgataaca tgggcaattg
    15841 cacgcaattc atgcagcgct tttaaggtgc gcgagcgctt aatacgcgac tccagcgaca
    15901 ccaggaaaaa taaactcgcc cctactaaaa ccagcgtatt taaaaatgcc tcggtcaact
    15961 gaacgaactc accaaaactc ggcactgatg gttgcaattt cacccagtga aaacccatca
    16021 acccagacat cacccccccc ataatcacac aagcaacccc gactcgaatc gagcgattgg
    16081 gccgtgtaat ccaggtaatg cgcgcctcgc tggcgcgggc aatggcattg agctccacac
    16141 atacatttaa caggccgcag tcggcaaagc gctcgctaat ccgcgcctgc aaaatatcca
    16201 gagtgtcggt aattttggtc aggtggagct tcttaaacat aggcgccgca gtctctcaat
    16261 aggtgggcgc acagtgtagc cgcattaggc gctggcctta agactaatgg cacggcacga
    16321 cacaaaccta agcccactgc agccgcctgg ggcatgctcg ccaagggttc aactaggttt
    16381 tttaggataa ttgacagata aatgaatgag gtgaaaccag acggaaatgg gtgccagcat
    16441 acgtgatggg ccacacattt ctgttaccgg gtggggaact gttacccgct aaggccgtca
    16501 gtaacagtta tcggcacata aggtaacgaa aacctagatc aaatccgaat atcagcggcc
    16561 catgaaagtc atatacattt gattttcatg ggcttttttt ttatggcacc ccgcctgcta
    16621 ttgcttaggt acaataacaa caataaagaa taataaaaac caactcgata actaataaga
    16681 acaataaata ataagaaact gaaatttaac caaacataac aacaataaat aataaaaaag
    16741 aagaatgatc cccggagcgg gcattgcgac acagcaacgg tatcggggac acggagatgt
    16801 ccttcattac acaataacaa gcgagattcc agcgggaagg ccacaagcct tcccgctttc
    16861 gtttgtggag cccaaaggcg gccagccgtg aattttggcc catgcccatg gtagacttca
    16921 cgccccaaat cttggcacaa ggcctgctga agacccatgc tcaatcgcat tattaaattt
    16981 attaccggca aaaaaggcag caaaggccct gcccaataca gcgtggatca attgcagatc
    17041 gacttaaacc gggtcagccg caatgccatc aaagtggtgg atatgctgca atcagctggc
    17101 tattccgctt atatagttgg cggcggcgta cgggatttac tgctgaattt aaagcccaaa
    17161 gactttgatg tggccaccaa cgcaacccct gagcaggttc gccaagtatt ccgctctgcc
    17221 ctcatcatcg gccgccggtt tcgcattgtg catgtgcgtt tcgggcggga aattatcgaa
    17281 gtcaccacct ttcgcgccca ccaccaggaa tccagtaacg ctaaagacgc aaaacaatct
    17341 gaccacggtt tgctgttgct ggataatgtg tacggcgatc ttgaatccga tgcgttccgc
    17401 cgcgacttca ccgttaacgc cctctaccta gacccaaaca cccgccaaat tattgatttt
    17461 gcagagggca tagaagacct aaaacaacgg cagctgcgca ttattggcga ggcggaagcg
    17521 cgctaccggg aggatccggt gcgtatgctg cgcgcggtgc gctttgccgc taagttgggc
    17581 tttcgcttgg aggcaagctc tgagcgcccc attagcgcct tagcaccact gctggcgcac
    17641 attccgcctg cgcgcatgtt cgaggaagtg ctaaaacttt ttttaagcgg cagtgccacc
    17701 gcatgttttc acctgctgcg ccaatacgac ctgttttatt acctcttccc cggcactgaa
    17761 gaggtgctga aaacccaaga ccccatcagc gaagccattg ccgagcgcgt ggtgatcaat
    17821 accgataagc gtatccgcag tgacctgcgg gtgacccccg cttttatcta cgccgttttc
    17881 ctctggctgc ccctgcaaca cgcggtgaaa aagctccaaa gccaaggggt ttcgccccag
    17941 gatgcgctgc aaaaagccgg ttccgatgta atcgccaagc agctatcgcg cacggcgatc
    18001 cccaagcgtt ttttaattcc cctgcgggag atttgggccc tgcagtggcg cctacctaag
    18061 cgcgatggcg gtaaagcctt cggcctactt gagcacccgc gttttcgcgc ggcctatgat
    18121 ttcctcctgc tgcgcgaaga ggccggggaa aacttggacg gtttggggca ctggtggaca
    18181 cagtttcaaa atgccgatag cgccagccgc gaagaaatgg tgaaaggact agaaagcccc
    18241 ggcaaacgta aaacccgcaa gcgccgcccg aaaaaacgcg gctccggcgc aagcccagag
    18301 gctgcccagg acgacacgga atgagtttca cccgtgtcta catcggcctg ggcagcaacc
    18361 tgcaagcacc catcgcacaa ctaactcaag cctgcaaggc tttaaagcag ctcgcccccg
    18421 cggacgattt tttatgctca cctttttacc aaagcaaagc cgtggggcct gctcagcctg
    18481 attacatcaa tgcctgcgcc acctttagca cagagcttgc gccgctggat ttattagatg
    18541 ccctgcaggc cattgaagcg cagcaagggc gcacccgcag cctgcattgg ggcccgcgca
    18601 cactggattt ggacctgttg ctctacggct ccttatgctt agataacccg cgcttacagc
    18661 taccccaccc ttatttgaaa gagcgc
//
LOCUS       DAOQGS010000007        18359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1409_length_18359_cov_4.318947, whole genome shotgun sequence.
ACCESSION   DAOQGS010000007 DAOQGS010000000
VERSION     DAOQGS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 18359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18359
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_18359_cov_4.318947"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..988
                     /locus_tag="PKZ52_00595"
     CDS             221..988
                     /locus_tag="PKZ52_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HNG58289.1"
                     /translation="MHSEPSYNLFLVALSFLVSVFGSFVALTLIRAVRSSDSAGKLPW
                     IIAAAFALGGGAIWTMHFIGMLAYDMGIPVSYNGPLTLASLAIAVVVVGLGIWVVGTA
                     DKSTARLIVAGVLTGLGVASMHYTGMAAMNGMSKMSYEPFWFSLSIVIALVAATAALW
                     LAFNLDGQWQLIGASVVMGIAVCGMHYTGMYGMIMSHDPDTLAQLEGIKPMQMGLMIF
                     CFSMLLLVLSLIAALSQLNRKMYEALEAEDDEVVPSP"
     gene            1497..3209
                     /locus_tag="PKZ52_00600"
     CDS             1497..3209
                     /locus_tag="PKZ52_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNG58290.1"
                     /translation="MKILILGSAFSGLCQRVLRELILMGHQVDQHYGMDEPVLRQQLA
                     LFQPELIICPFLTHRIPDDIWQSHRCLIVHPGIEGDRGASSLDWAIQTQSDEWGVTLL
                     QADAEMDAGAIWGTTNFPLRSAAKTSVYKREVSSAAVNMIKKAVSDFADPSFQPRPLD
                     YNNPAVRGRLLPTMKQQDRAINWQQDTSALIAAKINAADTTPGVLDELEGVPAHIFGA
                     VLEPNLTGKPGDIIAIKHGAVCKATVDGAIWIRQAKTKAIAGLAPIKLPAEYLAKHIF
                     SSHKISALNTAGQQGIYKDIWVEHQGTVAYVYFDFYNGAANTEQCQRLVACLKEVKAG
                     PSKVVVLMGGENFFSNGIHLNTIEAAADPALESWLNINAIDDVVKEIIHSPNQVFLAA
                     LRNNAGAGGAIVALACDKIIGREGVVLNPHYQNMGLFGSEYWTYLLPKRVGGELAAAI
                     THQCQPMLMAEALDIHLVDELFQEDWHSFHNQLERFAHSLAAPQVHAAILRHKIDSRQ
                     KDEAKKPLEDYRQEELAKMRAIFDNPNSYYHEARRNFVYKQPPKKPCWVDSSCIAKLE
                     LTHS"
     gene            complement(3381..4262)
                     /locus_tag="PKZ52_00605"
     CDS             complement(3381..4262)
                     /locus_tag="PKZ52_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58291.1"
                     /translation="MRFTLRQIEVFLAIAHFENVSRAASYLAMSQSACSGALIDLERQ
                     YARPLFDRLGKRLQLNDRGRLLRPKAQALLEQAKSFEGDLQQQQDVGQLKLGATLTIG
                     NYLAVPMIKDFMARAPSARVSLHVANTQAVVQGLLNYDYDLGLIEGDINHRDLILLPW
                     RSDELVCFAVSHHPLTRQLSVSDGDLINAPWILREQGSGTRQAFERAMQGLLPQLNIV
                     LELEHTEAIKRAVEAGMGIACLSRISLADAFARGSLAPIAVPQRDFSRRLYVALHKHK
                     YTSPSVLQWLQLCGLAL"
     gene            4387..5163
                     /locus_tag="PKZ52_00610"
     CDS             4387..5163
                     /locus_tag="PKZ52_00610"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNG58292.1"
                     /translation="MSNLLKARVLTVHHWNDTLFSFTTTRDPGLRFRNGHFCMIGLEQ
                     PSGKPLMRAYSIASANYEENLEFFSIKVQDGPLTSQLQKIQPGDEILINPKTTGTLTT
                     DFLLPGKNLWLLATGTGLAPFMSIIKDPEVYAQFDKVILTHGVRICSELAYEQFITEE
                     LPNNEFFGEEVREKLLYYPTVTREPFRNQGRLTDLLKTGKLTADLGLPPINLEDDRFM
                     ICGSPSMLKDFCAILNEGGFKEVRGSDPGHYVVERAFVEQ"
     gene            complement(5660..7120)
                     /locus_tag="PKZ52_00615"
     CDS             complement(5660..7120)
                     /locus_tag="PKZ52_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG58293.1"
                     /translation="MQQSTQSKLNVPNSSQKPINNAQDWSPNCARVVGCAFFMVLLDA
                     SVIANALPTMAASFHVRALDLSQGMSVYWLALALFAPLSGWVADRIGGRRVFVISLCV
                     FIVASMLCGWVNNVEQFIAARVLQGAAGAFMVPVGRLLVLQTTPKQQLVRAMATIAQP
                     ALIAPVIGPLLGGIFVTYFAWPWIFYLNLPLGVIVLLFALRFIPPSAKRVGEALDKRG
                     FIFTALALIGLLYGLDTFAHASLPTYLSAVLCGAGIVMALVAARHFKQAKQPLLNMQV
                     AQVQTYALSNLWVGTLVRTGINATPFLLPLYFQQAMGKSALAAGGYMLAYFLGNFGMK
                     SASVVLIKRFGFRTILVANGVLAGVSIMLLGTLSIIGFDIVIAIILLLAGLSRSLQYS
                     ALNSLAFADVPDAQRSAGTTLSYMTQQISLMLGVALSVLILNLVPGVASLTQVAALPF
                     GWAFAAMGLLVVVASLGFLRLAAGAGAEVSGFVARS"
     gene            7239..7310
                     /gene="pqqA"
                     /locus_tag="PKZ52_00620"
     CDS             7239..7310
                     /gene="pqqA"
                     /locus_tag="PKZ52_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HNG58294.1"
                     /translation="MWTKPAYEDLRIGFEVTMYFSFR"
     gene            7437..8348
                     /gene="pqqB"
                     /locus_tag="PKZ52_00625"
     CDS             7437..8348
                     /gene="pqqB"
                     /locus_tag="PKZ52_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HNG58295.1"
                     /translation="MKIRVLGSAAGGGFPQWNCNCPNCAGLRQGTLKATARTQSSIAV
                     STDERHWVVFNTSPDIRAQLEAFTAIQPKQGLRDTGIAAIVFMDSQIDHTTGLLMLRE
                     GCPHEVYCTDCVYEDLTSGFPIISMLKAWNGGINRHRINTGEPFIIPAIKGLLITPIF
                     LTSKAPPYSPHRHNPQPGDNIGVLLTDQTSGKSLFYAPGLGAIEPHLIPYMQQVDCLL
                     VDGTFWQEDEMAITGVGTKLAREMGHLPQSGADGMIQALKQFPQARKILIHINNTNPI
                     LNEESWQRQELTAQQIEVAFDGMEIEL"
     gene            8532..9272
                     /gene="pqqC"
                     /locus_tag="PKZ52_00630"
     CDS             8532..9272
                     /gene="pqqC"
                     /locus_tag="PKZ52_00630"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HNG58296.1"
                     /translation="MAPTPWNKEEFEARLRAKGKRYHIYHPLNQAMNSGQCTPEQIRG
                     WVANRFYYQVTIPIKDAAILANCPDRDVRRLWIQRILDHDGQLNAQGEFSEGGIEAWL
                     RLGEACGLSREEILDQSHVLPGVRFAVDAYLNFAKNASWQEAACSSLTELFAPTIHQN
                     RLDNWPTHYPWIEPAGYQYFRNRLKEARRDVDHGLAITLDYFATRAQQERALDILQFK
                     LDVLWSIMDAITMAYVHNTPPYHNCEAI"
     gene            9281..9571
                     /gene="pqqD"
                     /locus_tag="PKZ52_00635"
     CDS             9281..9571
                     /gene="pqqD"
                     /locus_tag="PKZ52_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNG58297.1"
                     /translation="MSDTLNLIPTLNPHYRFQWEEAQNCYVLLYPEGMIKFNGSAGEI
                     LAQVDGTRTIGDIIKTLNAKFPGIDGLENDVVEFFHDAFAKRWLQHQDLGAE"
     gene            9851..10957
                     /gene="pqqE"
                     /locus_tag="PKZ52_00640"
     CDS             9851..10957
                     /gene="pqqE"
                     /locus_tag="PKZ52_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HNG58298.1"
                     /translation="MPTQSPPLWLLAELTYRCPLQCPYCSNPINFANIKDELDTDTWI
                     KVLHQARTLGAAQLGFSGGEPLVRQDLSQLIKEARTLGYYTNLITSGVGMDGARVEEF
                     KRAGLDHIQISFQASSEELNNYFAGSSKAFANKKAMAAEVKRQGYPMVLNFVLHRHNI
                     DQVEHILELSLELGADYVELANTQYYGWALHNRAQLLPSIEQLKKAEAATQAFRQKVG
                     NKMKIIFVVPDYYETRPKACMNGWGKLFLTITPDGTALPCHSARDLPIQFPNVRDLAI
                     KDIWYNSAGFNYFRGDDWMREPCRSCPEKEKDYGGCRCQAFYLTGDAAAADPVCDKSP
                     SHGEIRDAVAQAQVIATDEVPLVFRNVENSKGFA"
     gene            11120..11647
                     /locus_tag="PKZ52_00645"
     CDS             11120..11647
                     /locus_tag="PKZ52_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58299.1"
                     /translation="MKTSNFIYCIFTLISTNCFAFTNSDRFKEKSNSADDVVIVRKIS
                     GQLDCHLFRQEIQKICNDKGFSERAMAYNQRKRKDEYIVLSSIYGKKLPGEVLEFNFE
                     DIAYHQTILGQDYLMFIDEYRGTLRYHPCDVFEYELLKSILDKNPSIETLFNHLKEKY
                     PNGSCPPDPKFNHAD"
     gene            12143..12610
                     /locus_tag="PKZ52_00650"
     CDS             12143..12610
                     /locus_tag="PKZ52_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58300.1"
                     /translation="MLFIRLFTLIVLSGISLSSFAGLCAVGQVKEVGTWINPDANTSG
                     ITKAIFKEECRDASTTVCNGNICSTTSAVKLVYTSELWGKCHPTDCYWGKVDGVYTSG
                     RWLRFYYDHGFAKRTVWGQVWSGGINWLDLIVDTDFVDPARADYRFRAWMQRL"
     gene            12778..13461
                     /locus_tag="PKZ52_00655"
     CDS             12778..13461
                     /locus_tag="PKZ52_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58301.1"
                     /translation="MGANIKLMGAALLVLANNIAWYYATDDKQPAAVQTVTAPTKSTA
                     QATTQEPINTPSLTIKNAPIAQHTHIAPAAPKTMELEANLPARESEQLIADQAFSKET
                     FTFDSPEQKIATIQQLAPQGEDLIFLQKIIESNDSDEVKIAALERLNGQQHFGALNTA
                     LTVLNKNNTNLSMAALGIIKNSRDTSLIPQLRSLALNAKNEMLQQEINATIGHLENSL
                     TMGMDFANR"
     gene            13743..14672
                     /locus_tag="PKZ52_00660"
     CDS             13743..14672
                     /locus_tag="PKZ52_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58302.1"
                     /translation="MIKLNFTKNLHLTEMLGPNPYAHLSAASGLVKIGKWLFIVADDE
                     KHIAVFNGEGDSSGIAHPIMAGELPLDTEARKRVKHDFEAIAQVPNSKKLPYGGLLIL
                     GSGSKKQREQGVIIPFLAPPTDSKIPALGEACIIDLDPIYDALKDEPGKTNIEGAIIL
                     GKEILLFQRGNKNNRNAIIRFKWKEFLASATGEGKGTPDYTIQHFDLGAIDGVPLCFT
                     DATALTDGRLVFTAAAENAEEACVDGACMGSAIGIISTTGELISLKRVDKKVKLEGID
                     VQEESTELKLWLVSDPDNPQLPGQLFTAQIKRD"
     gene            14907..15734
                     /locus_tag="PKZ52_00665"
     CDS             14907..15734
                     /locus_tag="PKZ52_00665"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNG58303.1"
                     /translation="MSNSDYARIASAITYIQANVKAQPDLADIARHVHLSPYHFQRLF
                     SRWAGTTPKRFLQVLTLELGKQLLSQNYSLLDTAEHLGLSSPARLHDHFVQIEGITPG
                     EFKSQNAALIIRYGLAETLFGLLFVAQTPRGICRAVFCDNADATGEITALTEQWPRAH
                     LIHAPEVAQDFAKRMFEIKNPTCANIALHVQGTNFQLAVWRALLRIPVGTAISYGDLA
                     HQLGKPSAARAVGTAVGANPIAVIIPCHRVIQQSGALGGYRWGLVRKQAIQVWERVE"
     gene            complement(15906..17183)
                     /locus_tag="PKZ52_00670"
     CDS             complement(15906..17183)
                     /locus_tag="PKZ52_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG58304.1"
                     /translation="MTQDKFIWGSRRRFLKQLSAASLMPAAINSALGSSGLLWARGAL
                     AAGAAPKRLLTIHVTNGAVPGTWHALGEGSNYTLPEASSPFNPYKNKCIFFQNITGAG
                     GHGPFYQCVAGEKRDSIDVYLGRKIGASTPFSSIQLGVNSSGGLSRLNGNDIPFEMSP
                     LKAFNRLFPASSGSGDLRTLQKKGIIGANNAMINELSSKLGSAQKIRMQEHLASLEKL
                     SQRIDQAAIAGQGQSAACKSPVWDGSFRDEAELNSNITLRADLHADLIALAFKCDTTR
                     IATLMYSDSGATYLAPEVETGDLHGAIHGYKGLERFTAYRKYFSQRIVYLMDQLAKTP
                     DMDGRTLLDNTLIYITSDMGDGGSHTNANTPVVLAGGAGGALVSGRSIVYGSETRLER
                     MLDTICLAMGADITAADYPGYGGAAGPLTGLLV"
     gene            complement(17217..>18359)
                     /locus_tag="PKZ52_00675"
     CDS             complement(17217..>18359)
                     /locus_tag="PKZ52_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG58305.1"
                     /translation="AADKDAYKLTPYELASFLSYTYTGSTPDDTLLAAAKAGLLETDA
                     QINAQIARLLATPRAQQQLGDFVAQWLRTDEVLTVTKDPAKYPELTAPIRAAMAQEVR
                     NLFTQVVLGQGENYSHFFAADFTYANADLAKYYGLSGATGSALIKVPAGSQRGGILTT
                     GAFLAVNGDPDESSPIRRAVRIRERLLCQDIDPPPPGISVSREAAEKALKERWESGQM
                     TNRERYAALTAAPYCQTCHEKRINPLGFGLEDYDTIGRYRSSDLNHLAIDANGIWYGI
                     TQLDGGDIQTFNGARDLGQKLAASDNVQSCLVANSFRFAMGTGHQIIDEVNALAGTLS
                     AQELNDYSCAVNDLARNMIANGANAKAMLQSLGSLKLVRYRKEQNR"
BASE COUNT         4958 a         4848 c         4222 g         4331 t
ORIGIN      
        1 tccgcgcatt ttcgtaggtt tagtgcggcg aaggcaaaat agatcaggac gatctatttt
       61 gccgcccggt taatagttac ggttaaagtt gccgtacagt tacagtgtgc agcggctaaa
      121 actcatccca gggaatgcat caggctgcgc aatccagcgt gctaatattg gttgatttgt
      181 taataactcc gtcctttttc atttctctcc aggcgtcctt atgcacagcg aaccttccta
      241 caatttgttc ctcgtggccc tatcttttct cgtctctgtc ttcggttcat ttgtggccct
      301 cactcttatc cgagcggtgc gcagcagcga ttcagccggc aaactaccct ggattatcgc
      361 cgccgccttt gccctcggcg gcggcgccat ttggaccatg cattttatcg gcatgctcgc
      421 ctacgacatg ggcattcccg tttcctacaa cggccccctc acgctggcat ccctggccat
      481 tgccgtagta gtggttggct tgggcatctg ggtggtgggt accgcggata aatccactgc
      541 gcgcctgatt gttgctggtg tacttacagg gctgggtgtg gccagcatgc actacaccgg
      601 catggcggct atgaatggca tgagtaaaat gagttacgag ccgttttggt ttagcctatc
      661 gattgttatc gcgttagtcg cagccactgc ggccttgtgg ttggcgttta acctcgacgg
      721 ccaatggcaa ttaatcggcg ccagcgtggt gatgggcata gccgtatgcg gtatgcacta
      781 caccggtatg tacggcatga ttatgtccca tgacccagac accctcgccc aattagaagg
      841 cattaaaccc atgcaaatgg gcttgatgat tttctgtttt agtatgttgc tgctggtgct
      901 cagcctgatt gccgccctca gccaactaaa tcgcaaaatg tatgaagcgc tggaggcgga
      961 ggatgacgag gtggtgccct cgccctagcc acatatttat agttttacgc tactttcatt
     1021 tatcgctcat ttcgtttatc gctactttcg ttccgccgct tttcccctcg ctgcctgcaa
     1081 ccttcctctt aacacaagag gaaggccttt cccctagtac tgaagccgtt taatcgccgc
     1141 tcaatccaat tattttgcgc ggtaacatat cggcagcggg ctcttgactt cgcacgataa
     1201 ttcagacaat tcacccatta ggcaaaataa ttgccaactc ccccgtcaaa aatgcacttt
     1261 tttaccattt tataacccat tatttaatac taaataacga ataaagcttg caacccatcc
     1321 agaaactaat ctaaattcac ctggccaatc tttagtggcc aggatcatca aaacggaatt
     1381 tcatcacaat aaaaacatga cctggcgcct aatttgcaaa ataataaaaa tgtaacacgg
     1441 ctcctacagc ctagttaccc ggcatttctc cctatcgatg aatttaagga aaccccatga
     1501 aaattctaat actcggttct gcattcagcg gattatgtca gcgtgttcta cgcgaactca
     1561 ttttaatggg tcatcaggtt gaccaacatt acggtatgga tgagcctgtt ctgcgccaac
     1621 aactggcttt atttcagccg gaactcatta tttgcccttt cttaactcac cgtatccctg
     1681 atgatatttg gcaaagccac cgctgcttaa ttgtgcaccc aggcatcgaa ggcgaccggg
     1741 gggcaagctc cttagactgg gccatacaaa cccaaagcga cgaatggggc gtaaccctgt
     1801 tacaggccga tgctgagatg gacgcaggtg ccatttgggg caccacaaat ttcccgttgc
     1861 gcagcgcggc taaaacctct gtgtacaaac gtgaagtgag tagtgccgca gtcaatatga
     1921 ttaaaaaagc cgtcagtgat tttgctgacc caagctttca gccgcggcca ctggattaca
     1981 acaaccccgc tgtgcgcggc cgcctattac ccaccatgaa acagcaggat cgcgccatta
     2041 attggcaaca ggacaccagc gctttaattg ccgccaaaat aaatgccgcc gataccaccc
     2101 caggtgtatt agatgaactt gaaggcgtgc ccgcgcatat ttttggcgcg gtgcttgaac
     2161 cgaatttaac cggcaaacca ggcgacatta ttgccataaa acacggcgcc gtctgtaaag
     2221 ccacagtgga tggcgccatt tggatccgcc aagcgaaaac caaagccatt gcggggcttg
     2281 cgccgataaa attaccggcg gaatacttag ccaaacatat ttttagcagc cacaaaataa
     2341 gcgcgctcaa tactgctggc cagcagggta tctacaagga tatttgggtt gaacaccaag
     2401 gcacagtcgc ctatgtgtat tttgattttt acaacggcgc agccaatacc gaacaatgcc
     2461 agcgcttagt ggcctgcctt aaagaggtaa aagcaggccc tagtaaagtt gtggtattaa
     2521 tgggtgggga gaattttttc agcaatggca ttcatttaaa tactattgaa gcagctgccg
     2581 acccggcctt ggaatcctgg ctcaatatca acgccatcga cgatgtggta aaagaaatta
     2641 ttcacagccc caaccaagta tttttagccg ccctgcgcaa taacgccggt gccggtggcg
     2701 ccattgtggc tttggcctgc gacaaaatta ttggccgcga aggtgttgtg ctcaaccctc
     2761 actaccaaaa tatgggttta tttggctcgg aatactggac ctatttattg cccaagcgag
     2821 tgggcggtga attagccgct gctattaccc accaatgcca gcccatgtta atggcggaag
     2881 ccttggatat acatttggtg gatgaattat tccaagagga ttggcacagc tttcacaatc
     2941 aattagagcg ttttgcccac agccttgcag ccccgcaagt gcatgccgcc atactgcgcc
     3001 ataaaattga tagccgccaa aaagatgaag cgaaaaaacc attagaggac taccgccaag
     3061 aggaactcgc caaaatgcgc gcgatttttg ataacccaaa cagttattac cacgaggcgc
     3121 ggcgcaattt tgtctacaaa caaccgccaa aaaagccctg ctgggttgat tccagctgta
     3181 ttgcgaagct tgaacttacc cactcgtaac accttgcggc ctagaacctc cgtgaggact
     3241 ctagcagctt gttgaaatac tgccctgcta gttcttggcg tcgccgccta ggtatttcaa
     3301 caagctgcta ggcttacatt gtttgttttt aactatttaa tctgcgcata accccacagc
     3361 gggttgcctt ttcaacagac ctaaagcgcc aacccgcaca gctgtagcca ttgcaaaaca
     3421 ctggggctgg tgtatttatg tttatgcagc gccacataca agcggcggga gaaatcccgc
     3481 tggggcacgg caatgggcgc taggctgccg cgagcaaagg catccgccag ggaaattcgc
     3541 gataaacagg cgatccccat cccggcttct accgcccttt taatcgcttc ggtgtgttcc
     3601 agctccagca ctatatttaa ttgcggcaac aacccctgca tagcgcgctc aaaggcttgc
     3661 cgtgtgccgg agccctgctc ccgtaaaatc cacggggcat taattaaatc cccatcgctg
     3721 acgctcaact gccgagttaa agggtggtga gagacggcaa aacaaaccaa ttcatcactg
     3781 cgccaaggca ataaaattaa atcccggtgg ttaatatccc cctcaattaa gcccaggtcg
     3841 taatcgtaat ttaacaaccc ctgcaccacc gcttgggtat tggccacatg caaactcacc
     3901 ctggcgctgg gggcgcgggc cataaagtct ttaatcatgg gcacggccaa ataattgccg
     3961 atggtaagcg tggcgccgag ctttaactgc cccacatcct gttgctgctg caaatcccct
     4021 tcaaaacttt tggcctgctc caataatgcc tgcgcttttg gccgcagcag ccgcccccga
     4081 tcgtttaact gcaggcgctt acccaagcgg tcaaacaggg gcctggcgta ctgccgctcc
     4141 aaatcaatca gggcgccgga gcaggcggac tgggacatgg ccaggtaact cgcggcgcgg
     4201 gagacattct caaaatgggc aatggccaga aacacctcaa tctgccgcag ggtaaagcgc
     4261 atatctggat ttccgattaa ggttataggt ataagttgat ttaccgctat gctagtaaac
     4321 gggtattatt tatgcaaacg agcgcatcct ccgctgccct gcatttgatt cacctaggta
     4381 ccctccatga gcaacttatt aaaagcccgt gtactcaccg tacaccactg gaatgacacc
     4441 ctgtttagct tcaccaccac ccgggacccg ggcctgcgtt ttcgcaatgg ccacttttgt
     4501 atgattggcc ttgagcagcc cagcggcaaa ccgctcatgc gcgcctactc cattgccagc
     4561 gccaattacg aagagaattt ggaatttttc agcattaaag tacaagacgg cccattaacg
     4621 tcgcagctgc aaaaaatcca acctggcgat gaaattctca tcaaccccaa aaccaccggc
     4681 accctcacca ccgatttttt attgcccggc aaaaaccttt ggttactggc caccggcacc
     4741 ggccttgcgc cctttatgag catcattaaa gacccagaag tctacgctca gtttgacaag
     4801 gtaatcctta cccacggcgt gcgcatctgc agtgaattag cctatgaaca gttcatcact
     4861 gaagaattac ccaataacga attcttcggc gaagaagtac gcgaaaaatt attgtattac
     4921 cccacagtta cccgcgaacc gttccgcaac caaggccgcc tgaccgattt attaaaaacc
     4981 ggcaaattaa ccgccgattt aggcctgcca cccatcaatt tagaagacga ccgctttatg
     5041 atttgcggca gccccagcat gctgaaagac ttctgtgcga ttttaaatga aggcggtttt
     5101 aaagaagtgc gcggcagcga cccaggccac tatgtggtgg agcgcgcgtt cgtggagcaa
     5161 taagccaaag cgtaagtaat gcctgcgaaa aacccgaagc tagtcttcgg gttttttatt
     5221 tgcgcgatga caattgcctg cgaaaaaccc gaagctagtc ttcgggtttt ttatttgcgc
     5281 gatgacaatt ttataaggcg gttttaaata atcctaaaaa actcagctga atccgtagcg
     5341 agagcgactg agggttagaa tatgggcgtt tctttcaaaa aattgagcga aagcagtagc
     5401 ccccttacgg cgagttcaat tcatcactgc aggtcgctgc aaccaataac tcgccaggtg
     5461 ttttctggtt ggctagaaaa tcacgtatag caccaataaa aggtgctgca cagttacaaa
     5521 aaagcaacaa ataattacta gcagcctgtt gcaatactac cttgcaagcg tgggcggcgt
     5581 taaattttgg ctgggcgccc agccaaaatt tgccttgcca gagcctcgcc gcctaggtat
     5641 ttcaataagc cgctggctat taagagcgcg ccacaaaccc cgacacttcc gcgccagccc
     5701 ccgccgctaa tcgtaaaaac cccagagacg ccaccactac caataaaccc atggcagcaa
     5761 acgcccatcc aaacggcagc gcggcaacct gtgtaaggct ggcaacccca ggcaccagat
     5821 ttaaaattaa tacgcttaag gcaactccca gcataagcga tatttgttgt gtcatataac
     5881 ttaacgtagt gcccgccgaa cgctgagcat ctggcacatc ggcaaaggcg agggaattaa
     5941 gggcagaata ttgcaaggag cggcttaagc ccgccagcaa taaaataatc gcgatcacaa
     6001 tatcaaaacc gataatactc aaagtgccta acagcataat ggacacacca gccagcacac
     6061 cgttggccac taaaatagtg cgaaacccaa agcgtttaat cagcacaacg cttgcggact
     6121 tcataccaaa attacccaaa aaatacgcca gcatatagcc cccagccgcc agggcggatt
     6181 tacccatggc ctgctgaaaa tacaagggca ataaaaaagg cgtggcgttg atacccgtgc
     6241 gcaccaaagt gcccacccat aaattgctca gggcgtaggt ttgcacctgc gccacctgca
     6301 tatttaataa gggctgttta gcctgtttaa aatgccgcgc cgcaaccagc gccatcacaa
     6361 tgcccgcccc acataaaacg gcacttaaat aagtgggcag gctggcatgg gcaaatgtat
     6421 ctaaaccgta caacaaaccg ataagcgcca aagccgtaaa aataaagccg cgcttatcta
     6481 aggcttcacc cacgcgctta gccgaaggtg gaataaatcg cagggcaaaa agtaaaacaa
     6541 taacacctaa aggcaggttt aaataaaaaa tccacggcca ggcaaaatag gtaacaaaaa
     6601 tcccccctaa cagtggccca atcaccgggg caattaaagc cggttgtgcg atagtagcca
     6661 tagcccgcac taattgttgt ttcggcgttg tttgcagtac taacagcctg cccaccggca
     6721 ccataaatgc accggctgca ccctgtaaaa cccgcgcggc aataaattgt tcgacattat
     6781 taacccagcc gcacaacata gatgcaacaa taaatacaca taaggaaata acaaaaaccc
     6841 tgcgcccgcc aatgcgatcc gccacccaac ccgaaagggg cgcaaataaa gccagcgcca
     6901 accaatacac actcatgccc tggcttaaat ccagcgcgcg cacatgaaaa cttgccgcca
     6961 tagttggcag cgcgttggca attaccgatg catccagcaa caccataaaa aaagcacagc
     7021 ccactactcg cgcacaattg ggcgaccaat cctgcgcgtt attaataggc ttctgtgatg
     7081 aattcggtac atttaattta gattgagtag actgctgcat aacttcttct aaaaataatt
     7141 ggccagcgat tgacagcgcc agcaatatga gcgagccttg aagccttgtg taccacacac
     7201 aaacttttcc aaccttcacc ccatttgagg aaacacctat gtggaccaaa cccgcttacg
     7261 aagatttgcg catcggcttt gaagtcacca tgtacttttc attccgctaa ttgttcgctc
     7321 acctgcaggt tgcggcgctc gctatcaaag tgtacacaga ttcgcgcgcc gcgtagtggg
     7381 ttgcaaaccg cgcacacagc cacagcctgc aggcatcatc ccccctcccc gcaactatga
     7441 aaatccgcgt actcggctcc gccgccggcg gtggctttcc ccagtggaac tgtaattgcc
     7501 ccaattgcgc aggcctgcgc caaggcaccc tcaaagccac cgcccgcacc cagtcatcca
     7561 ttgctgtttc taccgatgag cgccattggg tggtatttaa cacctcgccg gatatacgcg
     7621 cacaactgga agccttcacg gcgattcagc ccaaacaggg cctgcgggat accggcattg
     7681 ccgccatagt gtttatggac agccaaatcg accacaccac cggcttatta atgctgcgcg
     7741 aaggctgccc ccacgaggtg tattgcaccg attgtgtgta tgaggattta accagcggct
     7801 tccctattat cagtatgctc aaagcctgga atggcggcat taaccgccac cgcattaata
     7861 ccggcgagcc ttttattatt ccggccatta aaggcctgct gatcacaccg atttttttaa
     7921 ccagcaaagc cccaccctac tccccccacc gccataaccc gcaaccgggg gacaacatcg
     7981 gcgttttgct taccgaccaa acatccggca aaagcctgtt ttacgccccc ggccttggcg
     8041 ccattgaacc ccatttaatt ccctatatgc agcaggtgga ttgtttattg gtggacggca
     8101 ctttttggca ggaagatgaa atggcaatta ccggtgtggg cactaaactc gcccgggaaa
     8161 tggggcacct gccgcaaagc ggcgcagacg gtatgatcca agcgctcaaa caattccccc
     8221 aagcgcgcaa aattttaatt cacatcaaca acaccaaccc cattttgaat gaagaatcct
     8281 ggcaacggca ggaattaact gcgcaacaga ttgaggtggc gtttgatggt atggagatag
     8341 agctttagcg ttttattcat atgaattagg tcaattatta ggttttacct caaaaacctg
     8401 caacacaaaa ttctaatttt aaagctccct cccctttaca aggggagggt cggggtgggg
     8461 ttaaaaacct gatttccaca caattcaata tcgctaaatt aaccgcccta aaaatgcgcg
     8521 agaaataata aatggcacca accccctgga acaaagaaga attcgaagcg cgcctgcgcg
     8581 ccaaaggcaa gcgctaccat atttaccacc cgcttaatca agccatgaat tctggccagt
     8641 gcacgcctga gcaaattcgc ggctgggtag ccaaccggtt ttattatcaa gtcactatcc
     8701 cgattaaaga cgccgccatt ttggctaact gcccagatcg cgatgtgcgc cgcctgtgga
     8761 ttcaacgcat tttggaccac gatgggcaat taaatgccca aggtgaattc tctgaaggcg
     8821 gtatagaggc ctggctgcgg ttaggcgagg cctgcggcct aagccgggag gaaattctgg
     8881 accagagcca tgtattaccc ggtgtgcgtt ttgcggtgga tgcctattta aatttcgcaa
     8941 aaaatgccag ctggcaagag gcggcctgtt catccctcac cgaattattc gcccctacca
     9001 ttcaccaaaa ccgcttagat aattggccaa cccattaccc ctggattgaa cccgccggct
     9061 accagtattt tcgcaaccgt ttaaaagaag cccgccggga tgtggatcac ggcctggcaa
     9121 ttaccttgga ttattttgcc acccgcgcac aacaagagcg cgcactggat attttgcaat
     9181 ttaaattaga cgtactctgg agcattatgg acgccatcac catggcctat gtgcacaaca
     9241 cgccgcccta ccacaactgc gaagccattt aagagccacc atgagcgata cattaaacct
     9301 aatccccaca ttaaaccccc actaccgttt tcagtgggaa gaggcacaaa attgttatgt
     9361 attactctac ccagaaggca tgattaaatt taacggcagc gccggagaaa ttctcgccca
     9421 ggtggatggc acacggacaa ttggcgacat tataaaaaca ttaaacgcaa aattccccgg
     9481 cattgatggc cttgaaaatg atgtagtgga gttttttcat gatgcatttg ccaagcgctg
     9541 gctgcagcat caagacttgg gcgcagaata attttcaaac ccggcaggcg ccgaaattaa
     9601 tgcccgcacc tgttgcgaat gtctttaatt aaacacctgg cattaactcc tagcccttta
     9661 ctagtagcct ggatgcagcg cagcgcaatc caagaatcca cgcgcaaacc ctggattccg
     9721 ctgcgctgca tccaggctac gaaccatctg ccccctcccc ttacaagggg agggctgggg
     9781 aggggttaaa ctcaatcgct cgttttaaat aatgccaata taactccccc taaaagccaa
     9841 ggtgacacaa atgcccacac aatccccacc cctgtggctc ctcgccgaac tcacctaccg
     9901 ctgcccccta caatgcccct attgctccaa cccaattaat ttcgccaaca taaaagacga
     9961 gctcgacacc gacacctgga taaaggtatt gcaccaagcc cgcacactcg gcgctgcgca
    10021 attaggtttt tccggtggcg aaccactagt gcggcaggac ttatcccaat taattaaaga
    10081 agcccgcacc ctagggtatt acaccaattt aattacctca ggcgtcggca tggacggcgc
    10141 acgggtggaa gaatttaaac gcgcaggcct tgaccatata caaattagtt ttcaagcaag
    10201 ctcagaggag ctgaataatt atttcgctgg cagcagtaaa gccttcgcca ataaaaaagc
    10261 tatggcagca gaggttaaac gccagggcta ccccatggtg ctcaacttcg tattacaccg
    10321 ccacaatatc gaccaggtag aacacatcct cgaattatcc ttagaattgg gtgcagacta
    10381 tgtggaatta gccaacaccc aatactacgg ctgggcccta cacaaccgcg cgcaattatt
    10441 gccatctatt gaacaactta aaaaagccga agccgccacc caagcattcc ggcaaaaagt
    10501 tggcaataaa atgaaaatta tttttgtggt gcccgattat tacgaaaccc gccccaaagc
    10561 ctgtatgaat ggctggggta aactcttttt aaccatcacc cccgacggca ccgccctacc
    10621 ctgccacagc gcacgggatt taccgattca attccccaat gtgcgggatt tagctataaa
    10681 agatatttgg tacaactcag caggctttaa ttattttcgc ggcgacgact ggatgcgcga
    10741 accctgccgc agctgcccgg agaaggaaaa agattatggc gggtgtaggt gccaggcgtt
    10801 ttatttaacc ggggatgccg ccgctgccga cccagtttgc gataaaagcc catcccacgg
    10861 tgagattcgg gacgcggtgg cgcaggctca ggttattgct acggatgagg tgcccttggt
    10921 gtttcggaat gtggagaatt cgaaggggtt tgcgtaggtt gggcagtcat gcccaacatt
    10981 tgggtgttta tggtaagaat atttattttt ttgagctggg tattactggc aaacctacgc
    11041 gcccctgtca aatccgcgcc ctacaagaac ataaccagtc gattgcagcc ccgcatttaa
    11101 acgaaaaaaa gagacaatca tgaagacttc aaatttcatt tattgcattt ttacacttat
    11161 atcgaccaac tgctttgcct tcaccaattc cgaccgcttc aaggaaaaat ccaacagcgc
    11221 tgacgacgtc gtaatcgtca gaaaaatttc cgggcaactg gattgccatc tcttccggca
    11281 ggagatacaa aaaatatgca acgacaaagg attttctgaa agagctatgg cttacaatca
    11341 aagaaagaga aaagacgaat acatcgtttt atcaagcata tacggcaaaa aacttcccgg
    11401 agaagtgctg gaatttaatt ttgaagatat tgcttatcac cagaccattc ttggccaaga
    11461 ttatttgatg tttattgatg agtatagagg gactctgcgc taccatcctt gtgacgtgtt
    11521 tgaatacgag ctcttaaaaa gcattctgga taaaaaccca tctattgaga ccctctttaa
    11581 ccaccttaag gaaaaatacc ccaatggaag ttgcccacca gaccctaaat tcaatcatgc
    11641 ggactaatat aacgcgcaac ttagattggt tatttaaggg gtaggttgaa agagcgcagc
    11701 aacttccgag atttaaatag caatctcttg caccgagcac cccatgtcgg aaacgggtaa
    11761 cttggattga ttttttgagg aacgcgtaac ttagattaat catttcagga gtaggctgga
    11821 agagcgcagc gacatccaac attaaaatgc taacaccttt cgctgcccca tgtcggaagt
    11881 cgctgcgctc ttccaaccta cgtgtaaaaa caaccgagcc acaaatactt aattactcga
    11941 ataaaatccc ttccaataaa aactgccaaa aatacatcat ggctcatcag taaaaacccc
    12001 ataaccagga aaataagggg aattattcac agccccagcg ccattaatta acagctccac
    12061 caccccacac cccctagcat agcggctcct ctggcagcgg ttttaggctg aggtttcctc
    12121 aactaatatt aggagcaacc ctatgttatt catacgctta ttcactttaa ttgttttatc
    12181 tggaatcagc ctgtcatctt ttgcaggctt gtgcgccgtt ggccaagtaa aagaagtggg
    12241 cacctggatt aatccggatg ccaatacctc cgggattacc aaggctattt ttaaagagga
    12301 atgccgggat gcctccacca ctgtgtgcaa cggcaatatt tgcagcacca cctcagcagt
    12361 aaaactggtg tacacctcgg agctttgggg taagtgccat cctaccgatt gttattgggg
    12421 caaagttgat ggcgtatata cctccggccg ctggttgcgc ttttattacg accacggctt
    12481 tgccaaacgc accgtgtggg gccaggtgtg gtccggcggc attaattggc tggatttaat
    12541 cgtggatacg gattttgtgg acccggctcg cgccgattat cgctttagag cctggatgca
    12601 gcggctgtaa tacaggggct ataacacagc acatttaata cagtgccttt aacaacgacg
    12661 tttaatctgg gccgctgtaa acgaggcggc ccagatttaa ttcaactcca cacaacctct
    12721 gtgcaggcgc tcagtattac tccgtgcgca gccaattaaa ttattaaagg tgaatttatg
    12781 ggagccaata taaaactcat gggcgctgcc ctattggtac tagcgaataa tattgcctgg
    12841 tattacgcca ctgacgataa acaaccagcc gcggtgcaaa cggtaactgc gcccactaaa
    12901 agcaccgcac aggcaaccac ccaagaacct ataaataccc ctagcttaac cataaaaaat
    12961 gcacccatag cacaacatac gcatatcgca ccagcagccc ctaaaaccat ggagttagaa
    13021 gccaacctac cagcacggga gagtgaacag ttaattgccg accaagcctt tagcaaagaa
    13081 accttcacct ttgatagccc agagcaaaaa attgccacca tccaacagct tgccccacag
    13141 ggggaggatt taattttttt acaaaagatt attgaatcca acgatagtga tgaggtaaaa
    13201 atcgctgctc ttgaacgcct aaatggccaa cagcattttg gcgcactcaa tacagccctt
    13261 actgtattaa ataaaaacaa taccaaccta agcatggcag ctctaggcat tattaaaaat
    13321 agccgcgaca ccagtttgat ccctcaattg cgcagcctcg ccttaaatgc caaaaatgaa
    13381 atgctgcagc aggaaattaa tgcgaccata ggccatttag aaaacagcct taccatgggg
    13441 atggattttg ccaatcgcta gtgttagtag ctgtacggaa gcaatgaata cacatcattt
    13501 cagaccgtca aaaagaggga catttttgac gagcctatag ggatgtattc acggcgtgtc
    13561 tgcaatgatg tgtattcatt gccggtgccc aggtagtagc tgacaagatg cgtattctgg
    13621 cacggggcag tatttaccta gcaatccaaa catacccaga aatccaaaca tacccggcaa
    13681 gattgatata ttattgccgc tgcaaactca tttagggcac cccacccatt gcagcggccg
    13741 ctatgattaa actcaatttt actaaaaacc tacatttaac cgaaatgcta ggccccaatc
    13801 cttatgccca tttaagcgcc gccagcggct tggttaaaat aggcaaatgg ctgtttattg
    13861 tggcggatga tgaaaaacat attgcagtat ttaatggtga gggggattcc tctggcattg
    13921 cccaccccat tatggctggc gaattgccgc tggatactga agcgcgcaaa agggtgaagc
    13981 atgattttga agccatagcg caggtaccca atagtaaaaa gctcccctat ggcggtttat
    14041 taattcttgg ctcgggttca aaaaagcagc gagagcaagg ggtaattatt cccttcctgg
    14101 cgccgcccac cgattcaaaa attccagcgc tgggggaggc ttgcattatt gatttagacc
    14161 caatttatga cgccctcaaa gatgagccag gcaaaaccaa tattgaaggc gctattattc
    14221 tgggcaaaga aatactgctc tttcagcgcg gcaataaaaa taatcgcaac gccattatcc
    14281 gttttaaatg gaaagaattt ttagcatctg ccacaggtga gggcaaaggc acaccggatt
    14341 acacaatcca acactttgac ttaggggcaa tagacggtgt gcctctgtgt tttaccgatg
    14401 ccaccgcact cacagatggc cgcctggttt ttaccgctgc ggcagaaaat gctgaagaag
    14461 cctgtgttga cggcgcctgc atgggttcag ctattggcat catcagtact actggggaat
    14521 taataagcct taagagggtg gataaaaaag tgaaattgga gggtatagat gtgcaggagg
    14581 aatccactga actcaaatta tggctggtga gcgacccaga taacccgcaa ctgcccggcc
    14641 agctttttac cgcacagata aagcgcgatt aaaaaaggta accaccaggg ccatacatct
    14701 gagcaagatt cgggtggcag gcggaagatc gttcaggcaa gcttactagc tgtaccgaag
    14761 caatgaaaga ccgtcaaaat gagggacctt tttgacgagc ctacttagga ttgtattcac
    14821 ggcgtgtctg aaatgatgtg tattcattgc cggagcctag gcaataagcg ccccaatacc
    14881 cgcccaatta cagccattaa tgatatatga gcaactccga ttacgcgcgc atcgccagtg
    14941 ccatcaccta tattcaagcc aatgttaagg cgcagccgga tcttgccgat attgcccgcc
    15001 acgtgcattt aagcccctat cacttccagc gtttatttag ccgctgggcg ggcactacac
    15061 ctaaacggtt tttgcaggta ttaacccttg agctgggcaa acaactgctg agccaaaatt
    15121 attccctcct ggacactgcc gaacacctcg gcctgagcag ccccgcccgc ctacacgatc
    15181 attttgtgca aatagaaggc attacaccgg gagaatttaa aagccaaaat gccgcgctca
    15241 ttattcgtta tgggttagcc gaaacattat tcggcctttt atttgtggcg caaaccccaa
    15301 gaggtatctg ccgggcggta ttttgtgata atgcagacgc aaccggcgaa attacggcct
    15361 taaccgagca atggccccgc gcccatttaa ttcatgctcc tgaagttgcc caggattttg
    15421 caaagcgcat gttcgagatt aaaaacccca cctgcgccaa tattgcccta catgtgcagg
    15481 gcaccaattt tcaattagcc gtgtggcgag cgctgctgcg cattcctgtg ggcacggcta
    15541 tttcctacgg cgatttagcc catcaattag gcaaacccag tgccgcgcgg gcagtaggca
    15601 ctgcggtggg agccaatcct attgccgtga ttatcccttg ccaccgagta attcagcaga
    15661 gcggtgcatt gggggggtat cgctgggggt tagtgaggaa acaagctatt caggtgtggg
    15721 agcgcgttga gtagctctta gaccttagtg taagaacgca cttaataatg tatttattct
    15781 cagcgcaatt aacagcgcac aatattactg cgtatgtcac acgggcttcg cgagcgaaca
    15841 attaaaccta aaaaccgtca caaaaaaaag ggccaaatgg cccttgaata aatccaatcg
    15901 tatttttaca ccaataaccc ggttaaaggc ccagcagccc cgccataacc tggataatct
    15961 gcggcggtga tatccgctcc catagccaaa caaatggtat cgagcatacg ctctaaccgt
    16021 gtttcactac cgtacacaat agagcggccc gaaaccaatg cgccgccagc gccaccggct
    16081 aacaccacag gggtgttggc attggtgtgg gagccgccat cgcccatatc gctggtgata
    16141 taaataagtg tgttatccag caaggtgcgg ccgtccatat ccggagtttt tgccaattga
    16201 tccattaaat acacaatgcg ctggctaaaa tatttgcggt aggcggtaaa tcgctccagg
    16261 cctttatagc cgtgaatagc accgtgtaaa tcgccagttt ccacttcggg ggctaaatag
    16321 gttgcgccgg aatcgctgta catcagcgtg gctatacggg tggtatcgca tttaaatgcc
    16381 agggcaatta aatcggcgtg taaatctgcc cgcagagtaa tattgctgtt gagctctgct
    16441 tcatcgcgga aactgccatc ccacactggg gatttacagg cggcactttg gccctggccg
    16501 gcaatggcgg cttggtcaat gcgctggctg agtttttcta aactagccaa atgctcttgc
    16561 atacgaattt tttgcgcgct gcctaattta ctggacagct cattaatcat ggcattattc
    16621 gcacctataa tgcctttttt ctgcagagtg cgcagatcgc cactgccact gctggcgggg
    16681 aataaacggt taaaggcttt taatggactc atttcaaagg gaatatcatt gccatttaag
    16741 cgcgataaac cgccgctgct attcactcct aattgtatgg atgaaaatgg tgtgctggcg
    16801 ccaattttgc ggcccaggta gacatcaata gaatcgcgtt tttccccggc cacacactgg
    16861 taaaacggcc cgtggccacc ggcacccgtg atattttgaa aaaatatgca tttattctta
    16921 taggggttaa atgggctgct ggcctcgggc aaggtgtaat tactgccctc ccccagggcg
    16981 tgccaggtgc cgggcaccgc gccgttggtt acgtgaatag tgagcaggcg tttgggcgca
    17041 gcacctgcag ccaatgcacc ccgcgcccac aataaacccg atgacccaag ggcgctattg
    17101 atggcggcgg gcataaggct tgcggcgctt aattgtttta aaaatcggcg gcggctgccc
    17161 cagataaatt tatcctgtgt catataggaa ccctaaatat attcaattaa aaccgcttag
    17221 cggttttgct ctttgcggta gcgaaccagt tttaaactgc ccaatgattg caacatggct
    17281 ttggcgttgg cgccgttggc aatcatgttg cgcgctaaat cgtttaccgc acagctgtaa
    17341 tcatttaatt cctgtgcact taaggtaccc gccagtgcat ttacctcgtc aattatttga
    17401 tggccggtgc ccatggcaaa acggaaacta ttggccacta aacagctctg cacattatcg
    17461 ctggcggcaa gtttctggcc taaatcccgc gcgccattaa aggtttgtat atcaccgccg
    17521 tctagttgcg taatgccata ccagatgccg ttggcatcaa tggctaaatg gttaagatcg
    17581 ctgctgcggt aacggccgat ggtgtcgtaa tcctccaaac caaaacccaa aggattaatg
    17641 cgtttttcat ggcaggtttg gcaataaggg gcggcggtta aggcggcgta gcgctcgcgg
    17701 ttggtcatct ggccggattc ccaacgctct tttaatgcct tttccgccgc ttcgcgggag
    17761 acgctaattc ccggcggtgg cgggtctata tcctggcaca acaggcgctc gcgaatgcgc
    17821 acggctctgc ggatggggga tgattcatcc gggtcgccat tgactgctaa aaaagccccg
    17881 gttgttaaaa tgccgccgcg ctgactgcca gctggcactt ttattaaggc gctaccggtg
    17941 gcaccactta agccgtagta tttggctaaa tccgcattgg cataggtaaa atcagcggca
    18001 aaaaagtggc tgtaattctc gccttggccc agcaccactt gggtaaataa attgcgcact
    18061 tcctgagcca tggcagcgcg gataggtgcg gttaattcag ggtattttgc cgggtcttta
    18121 gtgacggtta atacttcgtc ggtgcgcagc cactgggcga caaaatcccc caattgttgt
    18181 tgcgcgcggg gcgttgccaa caggcgggca atttgcgcat ttatttgtgc atcggtttct
    18241 aatagcccgg ctttagcagc ggctaataaa gtgtcatctg gtgtactgcc ggtgtaggta
    18301 taacttaaaa aactcgccaa ctcgtagggt gttaatttgt aggcgtcttt atcggcagc
//
LOCUS       DAOQGS010000008        17250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1523_length_17250_cov_4.610701, whole genome shotgun sequence.
ACCESSION   DAOQGS010000008 DAOQGS010000000
VERSION     DAOQGS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 17250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17250
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_17250_cov_4.610701"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="PKZ52_00680"
     CDS             <1..1526
                     /locus_tag="PKZ52_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HNG58306.1"
                     /translation="NDDLPEEPPKEPGEPPTFYDTVVVQEAKKLSLQNYERPVKDPSK
                     ALTQVDYSQYRAIRFNTEKSLWRDQSPFEVQFFHPGFLYQDPLTINTLENDLAATVNF
                     DRSLFRYDGPAAPLAETPLPSNNFAGFRVHYSLNSAKYKDEFMVFQGASYFRLVGPGQ
                     AYGLSARGLAIDTAEPTGEEFPYFREFWLKKPGPNENTLTIYALLDSPSLAGAYKFEV
                     KPGRRVEVEVEGTLFARKDVKKLGIAPLTSMFVFGENSVVHHDDYRKEVHDSDGLQIL
                     TGSGEWIWRPLQNPVNLHVSAMQDKSPKGYGLMQRDREFDHYLDVEAHYHQRPSLWVE
                     PIGKWGKGAVELVEIPTDSETNDNVVAYWISAKPLLAKQKKHFRYRLKVLNETPPNAN
                     LGRVLYTTTGWAALPGQANPPPKSKRLFVVDFYSGDLEGVSGDMPVVPNLTTSSGKVS
                     DLMLQKIPGLERWRAYFKLEPEANKPVDMRLSLTLNGKPITETWTYVWYPESLPKSK"
     gene            1545..3479
                     /gene="mdoH"
                     /locus_tag="PKZ52_00685"
     CDS             1545..3479
                     /gene="mdoH"
                     /locus_tag="PKZ52_00685"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HNG58307.1"
                     /translation="MTQAVYSLRLSKASRKWRLFLYLLLSSVLTWYGIYLMFDVLRAN
                     DLNILEGILLLLFSLNFAWISFAFWNAILGFLLNLFKRDPISLKKQIEIPPDTSPITT
                     RTAVVMPVYNEDTKRITAGFEATLRDLAATGQIAQFDFFMLSDTTKAPIAQAEREAWQ
                     ALQERLGPELSARCFYRRREKNVRRKVGNLDDFLTRWGGHYEHMIVLDADSVMTGDCL
                     LTLVRTMQANPDAGLVQTVPIPVRQDTFFGRFVQFAAVLYSPMLATGLAFWQTNTANY
                     WGHNAIIRVKAFMETCGLPTLPGKAPFGGDILSHDFVEAAFLRRGGWSTYLLPQLEGS
                     YEEVPSNLLEYAKRDRRWVEGNIQHLALLDTKGLKLISRMHFAMGAFAYLSSPLWLIM
                     LALGSIDAVTRAVTQNVFFGVGYQLFPNWPIAKPQTVHLMLIITAVLLLLPKLLAIII
                     TLRDRRQQFGGAIKLVLGALLETLFAILVAPVTMTFHAYFVLTVFLGQKVTWDAQARE
                     GLLVPWGEAFKRTLFSSLAALVWGGVTLYFSPLFFYWLLPILIGLVLAAPAVRYSSSL
                     EGGKRLRRWGLFLVPSEVNEVPALRSLEEVEAQILADTQSREGAPVPALPPEIHQEMP
                     VQTIERYLRKQKVVQTALQH"
     gene            4224..5855
                     /locus_tag="PKZ52_00690"
     CDS             4224..5855
                     /locus_tag="PKZ52_00690"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNG58308.1"
                     /translation="MKHNLLLIACLSALLSACGGENTSSSSSSSSSSSSSSSSSSSSS
                     SGGAINYWGDKCQSCHGAKGEGTDGYAVLTSAIINRYSTQAALAKVIGDSMPIGQEGT
                     CKDVCADTTAAQLRKWFPPGTSSSSSSSSSSSSSSSGRSSSSSSSSGAVVMPPIDPNG
                     APVQTPKPNADSLGGQTPAFKNQTRVPEQKSPYAYNTTTVTSSLSAPWALAILPDGRF
                     LITEKPGTLVIVTPEGKVSSDILGVPQVVYTGNSIGVQGGLLDVTLAPDFATSRTLFL
                     SYTAPSNGKQATAVARARLSNDETRLEDVKVILTQNPEWNNTLQFGSRFAWDREGHLF
                     ITFGDRSDTAIRTSAQDNSTLIGKVARVNTDGTPVAGNPFIGQSGKRPEIWSYGHRNP
                     QGAAIHPVTGELWTVEHGAKGGDELNRPEGGKNYGWPTITYGIDYSGTKLGEGTAKAG
                     MEQPVYYWDPVIAPSNLIFYKGDMFSGWNGNLLIGALSTSVKGLVRLVLDGNKVVAEE
                     RILNSINRIRDLEVDAAGAIWVVTDGGQLIKVTKR"
     gene            6031..6732
                     /locus_tag="PKZ52_00695"
     CDS             6031..6732
                     /locus_tag="PKZ52_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58309.1"
                     /translation="MGDNQNNQDYFEHAIRALEAEIANVGTHLAIDAGARQLYAKQIQ
                     AMAAELRAQASSGRISWAKAAEQAQEMRNLIMELIRSRSTPVGLAMAQRLKSEGKTLN
                     ELIARKVQQLYGNNAVFSKLTVAEQNAVYGEIVLSAGRSNPKVTAAMGRLSYLGKGMI
                     VISVALSVYAVATAEDKVDAAGRELATTGAGIAGGVAGGALAGLACGPGAPVCVTVGA
                     FVGGALAAFGVSLAW"
     gene            6738..7199
                     /locus_tag="PKZ52_00700"
     CDS             6738..7199
                     /locus_tag="PKZ52_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58310.1"
                     /translation="MRLVPAAELSTRLLTPADDQQLAQSQLILGGQIHPQVFTGAVLE
                     ACIQVLDNYLFFLSDDIPHEDSLHIYRLDAHFALHESLSIGAAYCTGAFVLRQLIAPA
                     QVEFNFLGEGNWCLTLTPEPFTHVPFFSDPPGVRRPFCVRTRLKLKFTARS"
     gene            complement(7500..9353)
                     /locus_tag="PKZ52_00705"
     CDS             complement(7500..9353)
                     /locus_tag="PKZ52_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNG58311.1"
                     /translation="MQKTQQRYLFNGLAAALVVFMAAFFALKLPLHFNNLRAAATSNP
                     PQLTPAHETDNPILNIQRLEEKKAEIAEGLAPVHTHHQPEDIVPIPTLVEPPIQLENP
                     ANHPAAAAAPATLSAQAAKISGDHMELAPIPKAEHDSGAGIKSPEFRDRFTGFTENPA
                     IQVTAAPVSTFSIDVDTAAYSFTRRQLNSGFLPAKDAVRLEEMVNYFPYQYPLPESKK
                     TPFKTSISVLPSPFKAGNQLVHIGIKGYQIPSADIPRSNLVFLLDVSGSMDAPDKLPL
                     VRQSMSVLLDSLKPDDTVAIVVYAGAAGTVLAPTAVKEKTKILAAINSLQPGGATAGA
                     EGIALAYQLAEANFNAKGVNRIILATDGDFNVGPSDDKDLKDLVERKREKGIYLSVLG
                     FGQGNYQDAMMQTLAQNGNGIAAYIDTLSEAQKVLVQEATSALFPIANDVKIQVEFNP
                     ATVAEYRLLGYETRALKRQDFNNDSIDAGEIGAGHSVTAIYEITPLKGGNPLIEPSRY
                     STKPQVEKTTLNEYGFLRMRFKTPGEAKSQLIETPILIDGPQNPALKAEAEFATAVAG
                     FSQLLRGSAYSGAMTYDNVIELAQRSKGPDEYGYRTEFIQLVRKAKIAKAL"
     gene            complement(9796..10485)
                     /locus_tag="PKZ52_00710"
     CDS             complement(9796..10485)
                     /locus_tag="PKZ52_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58312.1"
                     /translation="MKLHTRLKPLGALCLLSLTAFLGACGGDNTTGVIDKSGHFTQAR
                     TYVSEQPGGGRCATPDGCFAQMTFAPDGSGTIIFTDIANRVNYAVKKDVLTTNLEGLG
                     DIPKTLKFDVLDNARSLVRQDTGAVFNLKTAAIAVYAATGALACEPNTGISISQSAQT
                     LTNAKIAVESSYCGYLANIVRPAVCGIPTDKVYVHLIAQDQVAAAQKLGFSLANKDTA
                     SQVTQTPCPTN"
     gene            10630..11781
                     /locus_tag="PKZ52_00715"
     CDS             10630..11781
                     /locus_tag="PKZ52_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNG58313.1"
                     /translation="MSGPWEMPKAPHVCLFAEECLRLAHTLGASQGIARRTNPFWSDV
                     MPCSLCVHAAPLQGNSSPRRFTNDRPKPKSASGSGLRILWEVLFRKSKDSVPRVAIPV
                     LPITPAQLHAAPNGSIYRLGHSTVLLKLRDKFWLTDPVFAERASPFKWFGPKRFHAPP
                     ITLKDLPPIEAVILSHNHYDHLDRAAVLALATKTQVFLTPTGVGDTLIKWGIDHKKVR
                     QFAWWQGCEIAGIQFTATPAQHFSGRHLWDGNANLWASWVIVDGAQRLFFSGDTGYFA
                     GFKTIGERFGPFDLTLLETGAYNKSWAYVHMQPEETIQAHLDLKGRWLLPIHNGTFDL
                     SLHAWYEPFERICALATEANIPLATPKMGEALLLSDPQPGDLWWQELKA"
     gene            11836..12177
                     /locus_tag="PKZ52_00720"
     CDS             11836..12177
                     /locus_tag="PKZ52_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58314.1"
                     /translation="MEMEELQKLGEWLCRSLLARDFETIAKNLGYALAGEIPAVDAIK
                     NDFDSALKESGGALECLRYDVAIKPFASGTPGFFNLIECRFAFSNANQEILAEIIENE
                     RGCYLEQISCV"
     gene            complement(12274..12576)
                     /locus_tag="PKZ52_00725"
     CDS             complement(12274..12576)
                     /locus_tag="PKZ52_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNG58315.1"
                     /translation="MTQLLWHPQAVSDRQAIVETLAEESEEIALRWDQDLEGKLAQYA
                     TQPASLPAGRVPGTLELVLLPHYVLVAQAPTAENPLTVLRILHTSKTWPEANSPLA"
     gene            complement(12573..13013)
                     /locus_tag="PKZ52_00730"
     CDS             complement(12573..13013)
                     /locus_tag="PKZ52_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58316.1"
                     /translation="MAQACQNSPSQPSHTPAASQATSARVVGAKGFWSVYLRNAEGEW
                     PLVLGQTMRVFRHFESVVVQLKNRGIHQFQVDIQQDQAADKEVVISPMRPKILKRAHS
                     AEAYDAWFRAQVQQSLGCARRLLNNREASELMAEFKHSRLEATA"
     gene            13260..13799
                     /locus_tag="PKZ52_00735"
     CDS             13260..13799
                     /locus_tag="PKZ52_00735"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HNG58317.1"
                     /translation="MKTHLFIDCIAYSLMCLFVCLFMAAPIAGHAEDIKAPEAQAVQQ
                     VINTLAPQETDDTLVSLAGILGVFGGPVCVLIVLIVQHYRSQMRLADFRREAIGKLID
                     AGKDVPESLLFFQDLNGSNQPHKNLQRALVNIGFGLGVLVFLWAVHSLAAGTFGLIFI
                     GIGAAQFITWHLSRGTQLK"
     gene            13813..14349
                     /locus_tag="PKZ52_00740"
     CDS             13813..14349
                     /locus_tag="PKZ52_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNG58318.1"
                     /translation="MSLSDQELILRALQKHDQRAFAQLVVRYQSQLRIWCRRLCNGDA
                     ALGDDLAQEVFIKAWGALAAFKMEAKFSVWLYRIAFNAAASKWRRKSLDWCDVDEIPE
                     FNDEQDAAVELINERALHSALEQLTQPQQIALRLCYEDDLSHAQAASIMNIPLGSLKT
                     HINRGKQRLKQLLADWSE"
     gene            14351..14683
                     /locus_tag="PKZ52_00745"
     CDS             14351..14683
                     /locus_tag="PKZ52_00745"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HNG58319.1"
                     /translation="MTEPTQHSFDTQLKQHLRNSTAYIADDGFSHALMNRLPARSVWP
                     LRSLGLLIACLGLILAVVFAGPVLLAPVFYWAAGLTAVGVIKIGLVMGAFILLVTTAW
                     MARQLDWL"
     gene            complement(14780..15226)
                     /locus_tag="PKZ52_00750"
     CDS             complement(14780..15226)
                     /locus_tag="PKZ52_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HNG58320.1"
                     /translation="MKPILAIILSFFILTPATYCVAATTRQIVPPPIEHTAAGNGAID
                     QAFEQGRSDLQVTGRGTVFKVLADDNDGSRHQKFLVRLPSGLTVLIAHNIDLAPRINN
                     LKVSDSIEFYGEYEWNEKGGVVHWTHRDPKGRHPAGWLKHKGQKYQ"
     gene            complement(15331..16221)
                     /locus_tag="PKZ52_00755"
     CDS             complement(15331..16221)
                     /locus_tag="PKZ52_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58321.1"
                     /translation="MDRFTEMQVFVAVAETEGFAAGARKLGVSPPVATRAVADLEARL
                     GVKLFTRTTRYVRVTDAGKRYLDDCKRILSEINEADDAAAGINGEPSGHIAITAPVLL
                     GRMFVQPVIIEYLYRYPKMDLSALYLDRVVNLLEEGLDVGIRVGELADSSIRAIGVGT
                     VRRVLCASPDYIAQMGLPQTPEDLLEHRLVSSNGVNPSSEWKFRHEKAVRVKPRLNVT
                     TNESAIDAAVAGFGIIRQMSYQVAAELAKGRLQIVLSEYELDPLPVHILHRESRYPSA
                     KIRSFVDLLAERLRANKSLN"
     gene            16368..16901
                     /locus_tag="PKZ52_00760"
     CDS             16368..16901
                     /locus_tag="PKZ52_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNG58322.1"
                     /translation="MSRINTVSQETATAEQSALFTAIQGKLGMVPNFLKVFANSPAAL
                     RAFLGLHSIASEGSLDLATRERIALLVAQQNACEYCVSAHTAIGRKAGLNSAEMEANR
                     AGSSQDAKAAVAVKLARSLIEHQGDITTAELIEARNAGYTDGDIVEIITHVGMTLLTT
                     ILGKASRVEIDFPKVSL"
     gene            16927..>17250
                     /locus_tag="PKZ52_00765"
     CDS             16927..>17250
                     /locus_tag="PKZ52_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNG58323.1"
                     /translation="MARAFADITFTPSVKAAQSVYGSRENNRGFEEADDPRNSLTASE
                     ADFIQARDSFYQATVGENGWPYVQHRGGATGFVKVLDARTLGYADFRGNRQYISVGNL
                     NADERI"
BASE COUNT         3802 a         4578 c         4618 g         4252 t
ORIGIN      
        1 ccaatgatga cctacccgag gagccgccga aagaacccgg cgagccgccc accttttacg
       61 acacggtggt ggtgcaggaa gctaagaagc tttcgttaca gaattatgag cgcccggtaa
      121 aagacccctc caaagcgctg acccaggtgg attattccca ataccgcgcc atccgtttta
      181 atactgaaaa atccctctgg cgcgatcagt cgccgtttga agtgcaattt ttccaccccg
      241 gctttttgta tcaagatccg ctcaccatca ataccctaga gaacgatctg gcggccacgg
      301 ttaattttga ccgcagcttg ttccgttacg acggccccgc cgcccccctg gcggaaacgc
      361 cgctgccgtc taataacttt gccggttttc gcgtgcacta ctcgctcaac tccgctaaat
      421 acaaagacga gtttatggtg ttccagggcg cctcttactt tcgtttagtg gggccaggcc
      481 aggcctatgg tctgtctgcc cgcggccttg ccatcgatac tgctgaaccc actggcgaag
      541 agtttcccta cttccgtgaa ttctggctga aaaaacccgg ccccaacgaa aacactctga
      601 ccatctacgc cctgttggat agcccctcct tggcgggcgc ctataagttt gaagtgaaac
      661 ctggccgccg cgttgaggtg gaagtggaag gcaccctgtt tgcgcgcaaa gacgtgaaaa
      721 aactcggtat agcgccttta accagtatgt ttgtgttcgg tgaaaactct gtggtgcacc
      781 acgatgatta ccgcaaagaa gtgcacgact ccgacggttt gcaaattctc accggttccg
      841 gcgagtggat ttggcggccc ttacaaaacc ccgtcaattt gcacgtctcc gccatgcagg
      901 ataaaagccc taaaggctac ggcttgatgc agcgggatcg cgagttcgac cattatttgg
      961 acgtagaagc ccactaccac cagcgcccca gcctatgggt ggagcctatc ggtaagtggg
     1021 gtaagggtgc tgttgaattg gtggagattc ccaccgactc ggaaaccaac gacaacgtgg
     1081 tggcctactg gatctctgcc aaacctctgt tagccaagca gaaaaaacac ttccgctacc
     1141 gcttaaaagt gctcaacgaa acgcccccca acgccaattt gggccgcgtg ctctacacca
     1201 ccaccggttg ggcggcgctg cccggccagg ccaacccgcc gccgaaatcc aagcgtttgt
     1261 tcgtggtgga tttctacagt ggtgatctgg aaggcgtgag cggcgatatg ccggtggtgc
     1321 cgaacctcac caccagtagc ggtaaggtga gcgatttaat gctgcaaaaa attccgggcc
     1381 tggagcgctg gcgcgcttat ttcaaactgg agccggaagc caacaaaccc gtggatatgc
     1441 gcctgagcct gaccctcaac ggcaaaccta tcacggaaac ctggacctat gtctggtacc
     1501 ccgaaagcct gcccaaaagc aaataacctg aagttaactc ccctatgacc caagcggtgt
     1561 attctctgcg cttatctaag gcgtcacgca agtggcgcct ttttctatat ttgctgttat
     1621 ccagtgtgct cacctggtat ggcatttacc tgatgttcga tgtattgcgg gctaatgacc
     1681 tcaatattct cgaagggatt ttgttgctgc tgttttccct caatttcgcc tggatcagtt
     1741 ttgccttctg gaacgccatc ctggggtttc tgctcaattt gtttaagcgc gacccgatca
     1801 gtttaaaaaa gcaaatcgag attccgccgg atacctctcc catcaccacc cgcaccgctg
     1861 tggtgatgcc ggtgtacaac gaggatacca agcgcattac cgcaggcttt gaagccaccc
     1921 tgcgggattt agccgccaca gggcaaatag cccagttcga cttctttatg ctcagcgata
     1981 ccaccaaagc ccccatcgcc caggccgagc gggaggcctg gcaggctctg caggagcgct
     2041 tggggcctga gcttagcgcc cgctgttttt accgccgacg ggagaaaaac gtacgccgca
     2101 aggtgggcaa cctggacgac tttctcaccc gttggggcgg ccactatgag catatgattg
     2161 tgctggatgc cgacagcgtg atgaccggcg actgcctgct caccctggtg cgcaccatgc
     2221 aggccaaccc ggatgcgggc ctggtgcaaa ccgtgccgat ccccgtgcgg caggacacct
     2281 tctttggccg ttttgtgcag tttgccgctg tgctctacag ccctatgctg gccacgggcc
     2341 ttgccttctg gcagaccaat accgccaact actggggcca caacgccatc atccgcgtga
     2401 aggcatttat ggaaacctgc ggcctgccca ccctgccggg caaggcgccc ttcggcggcg
     2461 atattctcag tcacgacttt gtggaggcgg cctttttgcg ccggggcggc tggagtactt
     2521 atttgctgcc gcagttggaa ggctcctacg aggaagtgcc ctcgaacctg ttggaatacg
     2581 ccaagcgcga ccgccgctgg gtggagggca atattcagca tttggctctg ctcgatacca
     2641 aaggcttaaa gctcatcagc cgcatgcact ttgccatggg tgcctttgct tatctgtcct
     2701 cgcccctctg gctgattatg ctggccctgg gcagcatcga tgcagtaacc cgcgcggtga
     2761 cccaaaatgt gttttttggc gtgggttatc agctgttccc caactggccc atcgccaagc
     2821 cgcaaaccgt gcacctgatg ttaattatca ccgccgtgct gctgctgttg cccaagctct
     2881 tggccatcat catcaccctg cgggatcgcc gccagcagtt tggtggcgcc attaagctgg
     2941 tgctgggcgc gctgcttgaa accctgttcg ctattttggt ggccccggtc accatgacct
     3001 tccacgccta ttttgtgttg acggtatttt taggccaaaa agttacctgg gatgcccagg
     3061 cccgcgaggg tttgctggtg ccctggggcg aggcgtttaa acgcaccctg tttagctccc
     3121 tggcggcctt ggtgtggggc ggcgttaccc tctatttcag ccctttgttc ttctattggc
     3181 tgctgccgat tttgattggc ctggtactgg cggcccccgc cgtgcgttat tcaagcagcc
     3241 ttgaaggcgg caaacggctg cgccgttggg gcctgttcct ggtgccctcg gaggtgaacg
     3301 aagtgcccgc cctgcgtagc ctagaagaag tggaagcgca gatcctcgcc gatactcaat
     3361 cccgcgaagg cgcgccagtg cctgcgctgc cgccggagat ccaccaggaa atgccggtgc
     3421 aaaccatcga gcgctacctg cgcaaacaaa aggttgtgca gacggctctg cagcactagg
     3481 cctgggcgcc attcttagcg gtgctttgcg ccgctgttgt ttccccttct catctcttaa
     3541 ggaaatggcg ggcggccatt ttcagccttt gtgcctaaaa ttcccctcca ttggaggggg
     3601 ggcaggcgtg cctgtcgggg tggttgtggt ggtgggccct gagcatttcc atttggcgag
     3661 cagttgttgg cgtgccccca tggcagcagg tcgcccacaa aaaactacca ccccacccct
     3721 gcggggcacc cctccgaaga aggggaattt tgcgctgcct gcggcgagca acgcctgtta
     3781 cacctatccc ctcaagccta cgcccccagt gtcattaggt taataatcct ccagaaaata
     3841 actgtagggg cgccccttgt gggtgcccgc tgccgcgtat ttccgctcga tagcccagac
     3901 atgcccctag ggcgcccccc aactcatcac tccctaagtg ttctgcctcc aggcgcgggt
     3961 aacaatatgc tttgggggtg aataagaatt gtcagatgat atgtggaaaa cgtgcggcta
     4021 tctgccgtga aaattcacta aatggtggct aaatgaaagt ttcctgtgcg gttcttcgca
     4081 ctgctgccaa tcccttctaa aaccaccctc aaactgcggc ttaggtcgca ggttttatta
     4141 tcgtatttta tctacactcg ccccatgctc agtgccagtg ctgagatttc agcccgttct
     4201 ccctaattac gtcgaggttt gcgatgaaac acaacctgtt gctgatcgcc tgtttgagcg
     4261 cgttgttgag cgcctgtggc ggcgaaaata cgtcttctag cagttccagc tcttcttcta
     4321 gttcgtcgag ttcttccagc tccagcagct catcttcggg cggcgccatc aattactggg
     4381 gggataaatg ccagtcctgc cacggcgcca aaggtgaggg cacggatggt tatgccgtgc
     4441 tgaccagcgc catcattaac cgctacagca cccaggcggc cttggccaag gtaattgggg
     4501 attccatgcc catcggccag gagggcacct gtaaggacgt gtgtgctgat accaccgccg
     4561 cccagctgcg caagtggttc cccccaggca cctcttccag cagctcctca tcgtccagtt
     4621 ccagcagctc atcctcgggc cgctcctcct cttcttcgtc cagctcaggc gctgtggtga
     4681 tgccccccat agaccccaac ggagcgccgg tacaaacccc caaacccaat gccgatagcc
     4741 tcggcggcca aacgccagcg tttaaaaacc aaacccgcgt gcccgagcag aagagcccct
     4801 acgcctataa caccactaca gttacatcca gcctaagcgc cccctgggcc ctggcgatac
     4861 tgccggacgg ccgctttttg attaccgaaa agcccggcac cctggtgatc gtaacccctg
     4921 agggtaaggt ctccagcgat attctcggcg tgccgcaagt ggtgtacacc ggcaatagca
     4981 tcggtgtgca aggcggccta ttggatgtga ccttggcgcc ggattttgct accagccgca
     5041 ccctcttttt gtcctacacc gcgcccagca atggcaagca ggccacggct gttgcccgcg
     5101 cccgcctgtc caacgatgaa acccgcttgg aggatgtaaa agtgatcctc acgcaaaacc
     5161 ctgagtggaa taacaccctg cagtttggca gccgctttgc ctgggatcgc gaaggccatt
     5221 tgttcatcac ctttggggac cgctcggata ccgccattcg cacctcggcg caggacaact
     5281 ctaccctcat cggcaaggtc gcccgcgtga ataccgacgg cactccggtg gcgggcaacc
     5341 cctttatcgg tcaatccggt aaacgcccgg aaatctggtc ctatggccat cgcaaccccc
     5401 agggcgcggc gattcacccg gtcaccggcg agctgtggac ggttgagcac ggtgcgaaag
     5461 gtggggatga gcttaatcgc cccgagggtg gtaaaaacta cggttggccc accatcacct
     5521 acggcattga ctacagcggc accaagctgg gggagggcac cgccaaggct ggtatggagc
     5581 agccggttta ttattgggac ccggtgattg cgccctccaa cctgattttc tacaagggcg
     5641 atatgttctc cggctggaac ggcaacctgt taatcggcgc tttgagcact tcggttaaag
     5701 gcctggtgcg gctagtgttg gatggcaata aggtggtagc cgaagagcgc attctcaatt
     5761 ccattaaccg cattcgcgat ttagaagtgg atgccgccgg tgctatttgg gtggttaccg
     5821 acggtggcca actaattaaa gtcaccaagc gataggcttt accccacagt caacggcgcc
     5881 acagggcgcc gtttttttgc ccgttaagta ttcgcttgcg gtggattctg cggcccatac
     5941 ccacagctaa accgccgctg aggttacaat cgcttaacct gcttgcagtt gttatacagc
     6001 tgtagcgata acccttttga gagcggcacc atgggcgata accagaataa ccaagactat
     6061 tttgaacacg ctattcgcgc cctagaggca gaaattgcca atgtgggcac ccatctggcc
     6121 atagatgcgg gtgcccgcca gttgtacgcc aagcagattc aggccatggc cgcagagctg
     6181 cgcgcccagg ctagcagcgg gcgtatcagc tgggctaagg ccgctgaaca ggcccaggag
     6241 atgcgcaacc tgattatgga attgatccgc tcccgcagca ccccggtggg cctggctatg
     6301 gcgcagcggc tgaaaagcga gggcaaaacc ctcaatgaac taatcgcccg caaagtgcag
     6361 cagctttacg gcaataacgc ggtgttcagc aaactcacag ttgcggagca aaatgctgtg
     6421 tacggggaga ttgtcctctc ggcggggcgc tccaacccca aggtcaccgc cgccatgggc
     6481 cgcttatcct atttgggtaa gggcatgatt gtgatctcgg tggccctgtc ggtttatgcc
     6541 gtggccacag cggaggacaa ggtggatgcc gctggccgcg aattggccac tactggcgca
     6601 ggtatcgcag gcggtgtggc cggtggtgcc ctggcggggc tggcctgtgg gccgggggcg
     6661 ccggtgtgcg tgactgtggg cgcctttgtg ggtggtgctc tggcggcctt cggcgtcagc
     6721 ctcgcctggt aatccctatg cgccttgtgc ccgccgccga actatccacc cgcctgctca
     6781 cccccgccga cgaccagcaa ttagcccaga gtcaattaat cctgggcggc caaatccacc
     6841 cgcaggtttt cactggcgcg gtgctcgagg cctgtataca ggtgctggat aattatttgt
     6901 tttttttaag cgatgatatt ccccatgagg acagcctaca tatctaccgt ttagatgccc
     6961 atttcgctct gcacgagagc ctcagcatag gcgccgcgta ttgcaccggg gcatttgtgt
     7021 tgcgtcaact gattgccccg gcgcaggtgg aatttaattt tttaggtgaa ggcaattggt
     7081 gtttaaccct aacccctgag ccatttaccc atgtaccgtt tttttcggac cctcccggtg
     7141 tgcggcggcc tttttgtgtg cgcacgcgtt taaaacttaa gtttacggcg cgctcctgaa
     7201 taaagtatgg ggattattta gaaattgctc gccattctgg ttggcaaaaa aattccctgc
     7261 cgttggggcg atggcattaa gttaaggatc ttgagctgtt ttccagaaga tttcgccccc
     7321 tgccagaaac caccacccag cctcccccct tagaaagggg gaggagcaaa aaacctaaaa
     7381 cgcagtaatt ttgcatgttt tcaaggccgt tttgctctct ccccttttga ttatgggtaa
     7441 acgcaaaaat cagccaaaaa gtgtgcgaaa cgcttagcct tacgacattg catgttattt
     7501 caaagagcct tagcgatttt tgccttgcgc accaactgaa taaattcagt tctataaccg
     7561 tattcatccg gccccttgct gcgctgcgcc agttcaatga cattatcgta ggtcatagcg
     7621 ccgctgtagg cgctgccgcg cagcagttgg ctaaaacctg ccaccgcagt tgcaaattcc
     7681 gcttcagctt taagcgcggg gttttgtggg ccatcaatta aaatcggcgt ttctattaat
     7741 tgcgattttg cttcgccggg ggttttaaag cgcatacgca aaaagccgta ttcattcaac
     7801 gtggtttttt cgacttgggg tttagttgaa taacgactgg gttcaatcag cggattgccg
     7861 cctttaagcg gggtgatttc ataaatcgcc gtgacgctgt gcccggcgcc aatctccccg
     7921 gcatcaatgc tgtcattatt aaaatcctgc cgttttaaag cgcgggtttc gtagcctaac
     7981 aagcggtatt cggccaccgt ggcggggtta aattccactt gaatttttac atcattggcg
     8041 atgggaaata acgccgaagt cgcttcttgc accaaaactt tttgcgcttc gcttagggtg
     8101 tcgatatagg cggcaatgcc attgccgttt tgcgccaggg tttgcatcat ggcgtcttgg
     8161 taattgccct gcccaaaacc taataccgaa agataaatgc ctttttcccg tttgcgctcg
     8221 accaaatctt ttaaatcctt atcgtcgctg ggccccacat taaagtcgcc atcggtggct
     8281 aaaataatgc ggttaacacc tttggcatta aaattcgcct ccgccaattg ataggcaagt
     8341 gcaatgccct cggcacccgc tgtagcgccg ccgggttgta ggctgttaat agcggcgagg
     8401 attttggttt tttctttcac tgcggtaggc gctaatacgg tgcccgcagc gcccgcgtac
     8461 accactatgg ccactgtgtc gtcaggcttt aagctgtcca ataaaacact catagactgg
     8521 cgcaccaggg gcaatttatc cggcgcatcc atggagcctg aaacatctaa taaaaaaact
     8581 aaattgctgc ggggtatgtc tgcactgggg atttgatagc ccttaatgcc gatatgcacc
     8641 aattgattgc ccgctttaaa ggggctgggc agcacactga tgctggtttt aaaaggcgtt
     8701 tttttcgatt cgggcagcgg atactgatag gggaaataat tcaccatctc ttctaaacgc
     8761 acagcatctt tagccggtaa aaaaccgcta ttgagctgcc ttcgggtaaa gctgtaggcg
     8821 gctgtatcca cgtcgatgga aaatgtggat acgggtgcgg cagttacttg aatcgcgggg
     8881 ttttcagtaa aacctgtaaa acggtcgcga aattcaggcg atttaattcc tgctccgctg
     8941 tcatgttcag ctttaggaat cggcgccaac tccatatggt cgccgctgat ttttgccgcc
     9001 tgagcgctaa gcgttgcagg cgcagctgct gccgctggat ggtttgccgg gttttccaac
     9061 tggatgggag gctccaccag ggtgggaatg gggacaatat cctccggttg gtggtgggtg
     9121 tgtacaggcg ctaagccctc ggctatttcc gcttttttct cttcaagccg ctgtatattc
     9181 aaaatggggt tatcggtttc atgtgcagga gtaagctgcg gtgggtttga agttgcagct
     9241 gcgcgcaggt tattaaaatg caagggaagt ttgagggcaa aaaaggccgc cataaacacg
     9301 accagcgctg cagccaggcc attaaataaa tagcgttgtt gggttttttg catgggatac
     9361 tcctaccggt gggtgatgta ggagtaagac gagggtgggc gctaaatcct tggctttggg
     9421 gcaaaaaatt tattaactat ttggcatgac ttattaggct ttccgggttg ctctcttcac
     9481 gcgggcggca aattgagagg tctgaaggat agttgattcg cggtgtagca ggggtgaata
     9541 caggttattg cagaccgtca aaaagaggga cctttttgac gagcctacac ggatgtattc
     9601 acggcgtgtc tgcaataacc tgtattcacc cctgcggggg actattgagg ccttgcagca
     9661 gtaaagtgct cgcctcatta cctagcagct tgtcgaaata ctactttgcc agcacgtcgc
     9721 cgcctgagta tttcaacagg ctgctaagca cctgaaaacc cggcgattaa atctcaccgg
     9781 gtgtaaagca taaaattaat tagtggggca gggggtttgg gttacttggc ttgcggtatc
     9841 tttattggcg aggctaaagc ccagtttttg cgcggcggcc acttggtctt gagcaattaa
     9901 atgcacatac accttatccg tgggaatgcc gcacaccgca gggcggacga tattggctaa
     9961 ataaccgcaa taactggact ccacggctat ttttgcattg gtgagggttt gggcggactg
    10021 gctgatacta atgcccgtat tcggctcaca agccagtgcg cccgttgccg cgtacacggc
    10081 gatggcagcg gtttttaaat taaataccgc gcccgtatcc tgccgcacca ggctgcgggc
    10141 gttatccaag acgtcaaatt ttaaggtttt cggaatatca cccaggcctt caaggttggt
    10201 agtgagtacg tcttttttaa ctgcataatt cacccggttg gcgatgtcgg taaaaataat
    10261 cgtaccgcta ccatccggtg caaaggtcat ttgcgcaaaa cagccatcag gagttgcgca
    10321 gcggccgccc cccggttgct cgctgacata ggtgcgcgcc tgggtgaaat ggcctgattt
    10381 atcgataacg cccgtggtgt tatctccgcc gcaggcacct aaaaaggccg taagactgag
    10441 taaacatagg gcacctaagg gtttaagtcg tgtgtggagt ttcataaacg atccttaagt
    10501 ctggtgggcg gggtgggttg cagcatgcac ctttggcacg gggcgcgcca tgaaccggtc
    10561 aacaggttac atattttaac aattagccgc tttccatggg ccgccgcagg ggaggcggtt
    10621 ggctaagcga tgagcggccc ttgggaaatg cccaaggcac cccatgtttg cctctttgcc
    10681 gaggagtgct tgcgcctggc gcatacacta ggcgcctcac agggcattgc ccgccgtacc
    10741 aacccatttt ggagtgacgt tatgccctgt tctttatgtg tccacgccgc gccgttgcag
    10801 gggaattcca gcccgcgcag gtttaccaac gaccgcccta aacccaagtc cgcatcgggg
    10861 agcggtttgc gcatcttatg ggaggtgtta tttcgcaagt cgaaggattc cgtgccccgg
    10921 gttgctatcc cggtactgcc gatcacccca gcgcagttgc acgcggcacc caatggcagt
    10981 atttaccgct taggccactc caccgtgtta ttaaaactgc gcgataaatt ttggctgacg
    11041 gacccggtat ttgcagagcg cgcctcgccc tttaaatggt tcgggcctaa gcgctttcac
    11101 gcaccgccca taacgcttaa ggatttaccg cccattgagg cggtgatttt atcccacaat
    11161 cattacgatc atttagaccg cgcggcggtg ttggctcttg cgactaaaac ccaggttttt
    11221 ttaacgccga cgggggttgg cgatacgcta ataaagtggg gtatcgatca taaaaaagtg
    11281 cgccagtttg cctggtggca gggctgcgaa atagctggca ttcaatttac cgcaactcca
    11341 gcccagcatt tttccggccg tcatttatgg gatggcaatg ccaatctgtg ggcctcttgg
    11401 gtaattgtgg acggtgccca gcgcctattt ttcagcggtg atacgggtta ttttgcaggg
    11461 tttaaaacta tcggcgagcg ctttggccca tttgatctca ccctattgga aaccggtgcc
    11521 tacaacaaaa gctgggccta tgtgcatatg cagccggaag aaaccattca ggcccatttg
    11581 gatttaaaag gccgctggct gctgccaatt cacaatggca cttttgattt atcgctgcac
    11641 gcttggtatg agccgtttga gcgtatctgc gctttggcaa cagaggctaa tattccactg
    11701 gccactccca aaatggggga ggctctgttg ttaagtgatc cgcaaccggg ggatttgtgg
    11761 tggcaagaat taaaggccta aaagaggata agtagggcta ttattccttc tatttataaa
    11821 tctgtggaat aaattatgga aatggaagag ctgcaaaaac taggcgaatg gctttgtcgc
    11881 agtcttctcg cgagggattt tgaaacgatt gcaaagaatt tagggtatgc tctggctgga
    11941 gaaataccgg cggtggacgc aattaaaaat gattttgaca gcgcgttgaa ggaatcgggt
    12001 ggcgcccttg agtgtttaag atacgacgtt gcgattaaac cctttgcaag cggtacaccg
    12061 ggttttttta atttaattga atgcaggttt gccttttcaa atgccaatca agagattttg
    12121 gcagaaatta ttgaaaacga aagaggatgt tacttagagc aaattagttg tgtttaaacc
    12181 taattaaaga cttcctttga aacattcacg cgagctcaca agccgcaatt ctgatacaca
    12241 taaaaagcgc aaaattgcag ccttgttggg ctgctaagcc agcggcgagt tggcttccgg
    12301 ccaagtttta ctcgtatgca aaatccgcaa aacggttaac ggattttccg ccgtgggcgc
    12361 ctgggccact aaaacataat ggggtagcag cacaagctcc agggtgcccg gcactcgccc
    12421 ggctggcagg cttgccggtt gggtggcgta ctgagcgagt ttcccctcca aatcctgatc
    12481 ccagcgcagc gctatctcct cagattcctc cgccagagtt tccacaatcg cctggcgatc
    12541 gctcaccgcc tgggggtgcc aaagcaattg cgtcatgcgg ttgcctcaag cctgctgtgt
    12601 ttaaattccg ccattaattc actggcctcc ctattattta aaagcctgcg cgcgcagcct
    12661 aggctctgtt gcacctgggc gcgaaaccag gcgtcgtagg cctcggcgct gtgggcgcgc
    12721 ttgagaatct tcggccgcat ggggctgata acaacttctt tatcggcggc ttgatcctgc
    12781 tgtatgtcca cctgaaattg gtggatgccg cgatttttta gctgcacaac cacggattca
    12841 aaatgccgaa agacgcgcat ggtttggccg agaaccagcg gccattcgcc ttcggcattg
    12901 cgcagataca cgctccaaaa gcctttggcg cccaccacgc gggcactggt ggcctggctt
    12961 gctgccggag tatgggaggg ttgagagggg gagttttgac atgcttgcgc catgggtagt
    13021 acctctgcac ggttttgtta tgtcccgccg ccggtttggg ccgggggttt tgtaacagcg
    13081 tgttaacacc atttgcgggg cggatagtag cggctctgcc ccagctgttg tgtgaagtta
    13141 tgatgacagc ggataaccgg gcgacagcat taaggcttta agatatgcag aattaaggtg
    13201 aatccaaacc catggcaagg cgcgtcttag agatacagac aacaaacttg gagcacttta
    13261 tgaaaaccca cctttttatc gactgcatcg cctacagcct tatgtgtctg ttcgtgtgtc
    13321 tatttatggc cgcgccgatt gcaggtcatg ccgaggatat taaagcccct gaagctcagg
    13381 ccgtgcagca ggttattaat actctggcgc cacaggaaac cgacgatacg cttgtttcgc
    13441 ttgcgggtat tttgggagtt tttggcggcc cggtgtgtgt attgattgta ttaattgtgc
    13501 agcactaccg cagccaaatg cgcctggcgg attttcgccg cgaggccata ggtaaattaa
    13561 ttgacgccgg taaagacgtg ccggagagcc tattattttt tcaggattta aacggcagta
    13621 accagcccca taaaaatttg cagcgggctt tggtgaatat tggttttggt ttgggtgtat
    13681 tggtttttct ctgggcggta cacagcctgg cggcaggcac ttttggctta atatttatcg
    13741 gtattggtgc agcacaattt attacttggc atttatcgcg cgggacgcag ctgaaataat
    13801 ggagcggtaa ctgtgtcctt atccgatcag gagcttattc tgcgcgcgct gcaaaaacac
    13861 gatcagcgcg cgtttgcaca gttggtagtg cgctatcaat cccaactgcg aatatggtgt
    13921 cggcgcctgt gtaacggtga tgctgcgctg ggggatgatt tggctcagga ggtatttatt
    13981 aaagcctggg gggctctggc ggcatttaaa atggaggcta aattttccgt ctggttatac
    14041 cgcatcgctt ttaatgctgc cgccagtaaa tggcggcgca aaagcctgga ttggtgtgat
    14101 gtggatgaaa taccggagtt taacgatgag caggacgctg cggttgaatt aatcaatgag
    14161 cgcgcattac actcggccct agagcaatta acccagcccc aacaaattgc cctgcgcctg
    14221 tgttacgaag acgatttatc ccatgcgcaa gccgccagta ttatgaatat tcccttaggc
    14281 agtttaaaaa ctcatatcaa ccgcggtaaa cagcgtttaa aacaattgct ggccgattgg
    14341 agtgaataat atgactgaac ccactcaaca ctcgtttgat acgcagttaa aacagcattt
    14401 gcgcaatagc accgcctata ttgccgatga cggttttagc cacgcgctta tgaatcgtct
    14461 acccgcgcga tcggtgtggc cgcttcgctc attggggctt ttgattgcct gtttaggcct
    14521 gatactcgcc gttgtatttg cagggcctgt gttgttggcg ccggtttttt attgggctgc
    14581 ggggcttacg gctgtgggtg tgataaaaat tggcctggtt atgggcgcgt ttattttatt
    14641 ggtaacaacc gcctggatgg cgcggcagct tgattggctt tgaatactct gcggcagcct
    14701 ttgaagtttt ttgtgcctgg gtgccagtgg gcgcaaagaa ataggtgttg gaacagtgcg
    14761 cacgccgcca attgcttttt tattgatatt tttgcccctt atgttttaac cagccggccg
    14821 ggtggcgccc tttggggtct cggtgggtcc agtgcaccac accacctttt tcattccact
    14881 catattcgcc gtaaaactca atactatcgc tgacttttaa attattaatg cgcggcgcta
    14941 aatcaatatt gtgggcgatt aaaaccgtta gcccggaggg cagccgcacc aggaattttt
    15001 ggtgcctact gccatcattg tcatccgcca gtactttaaa tactgtgcca cgcccggtca
    15061 cttgtaggtc gctgcggcct tgttcaaatg cttgatcaat ggcgccattc cccgcagcgg
    15121 tgtgctcgat gggtggcggc actatttgcc ttgttgtggc tgccacacag taggtggcag
    15181 gtgtaagaat gaaaaaactc aaaatgatgg caagaatcgg tttcattatg ggcctgcgca
    15241 aaaaataaat gagagggtaa gtttaagtgt aatgtgcaac cctgcgacaa gggttggctt
    15301 aagcggttaa attgtgcgct cacacacctt ttaatttaaa gatttattcg cccttaatcg
    15361 ctccgccaat aaatccacaa aactgcgaat tttcgccgat ggatagcggc tttccctgtg
    15421 taatatgtgc acgggtaatg ggtctaattc atattcgctc aatactattt gcagcctacc
    15481 ttttgccagt tctgcggcca cctgataaga catttggcga atgatgccaa agcctgccac
    15541 agcagcatca atggcggatt cattggtggt gacatttaag cgcggtttga cgcgcacggc
    15601 cttttcatgg cgaaatttcc attcgctact ggggtttacg ccgtttgacg aaacaagccg
    15661 gtgttctaat aaatcctctg gtgtttgtgg caaacccatt tgcgcaatat agtcggggga
    15721 ggcgcacagc actcggcgca cagtgccaac acctatagcg cgaatactcg aatcggctaa
    15781 ttcacccacc cgtatgccca catccaaacc ttcttccaac aaattaacca ctcggtccag
    15841 atacagcgca cttaaatcca ttttcggata acggtacaga tattcaataa tgaccggctg
    15901 cacaaacatg cgcccgagta acaccggggc ggtaatagca atatgcccac tgggctcgcc
    15961 gttaatgcct gcggcggcat catcggcttc atttatttca cttaaaattc gtttgcagtc
    16021 gtctaaataa cgcttgcctg catcggttac ccgcacgtaa cgggtggtgc gggtaaacaa
    16081 tttcacacct aggcgcgctt ctaaatcggc caccgcgcgg gtggccaccg gtggggatac
    16141 gcccagtttg cgtgcaccgg cggcaaaccc ttccgtttcc gctacggcaa caaatacctg
    16201 catttcagta aatcggtcca acggcggctc ccaatattcc agttttagaa ataatcaatt
    16261 gctttgtgtg agtattcttc atttgggcgg cccttggcaa gatgccctca ctcgccaagc
    16321 agccgcttgg caagacaacc taaattcact acagaagaga aaatactatg agccgaatta
    16381 atactgtgtc ccaagaaact gccaccgctg aacaaagcgc cttattcact gccattcaag
    16441 gcaaattggg catggtgcca aatttcttga aagtatttgc caattcccct gcggcattgc
    16501 gcgcattcct cggtttgcac agtattgcca gtgaaggcag tttggatttg gcaacgcgcg
    16561 agcgcattgc tttattggtt gcacagcaaa acgcctgcga atattgcgtt tcagcccaca
    16621 ccgccatcgg ccgcaaagca ggcttaaata gcgcggaaat ggaagcaaac cgcgccggca
    16681 gcagccagga tgccaaagcg gcagtagccg tgaaattggc acgctcttta atagagcatc
    16741 aaggtgatat tactacggca gaattaattg aagcgcgtaa tgctggttat accgatggag
    16801 atattgttga aattattacc catgtcggca tgactctgct caccacaatt ttaggtaagg
    16861 ctagtcgagt ggaaattgat tttcctaaag ttagccttta attaaacaaa acaaccgggg
    16921 gttatcatgg cgcgtgcatt tgcagacatt acttttacac ccagtgttaa agcggcgcag
    16981 agtgtttacg gtagccgtga aaacaaccgg ggctttgaag aggcggatga cccgcgcaat
    17041 tccctgacgg cttcagaggc tgattttatt caggcgcggg atagttttta tcaagccacc
    17101 gtgggtgaaa atggttggcc ctatgtacag catcgcggcg gcgcgaccgg atttgtcaaa
    17161 gtgctggatg cgcgcacttt aggttacgcc gattttcgcg gtaatcgcca atatattagt
    17221 gttggcaatc tcaatgcgga tgagcgtatt
//
LOCUS       DAOQGS010000009        17218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1529_length_17218_cov_4.604615, whole genome shotgun sequence.
ACCESSION   DAOQGS010000009 DAOQGS010000000
VERSION     DAOQGS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 17218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17218
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1529_length_17218_cov_4.604615"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2346)
                     /gene="secA"
                     /locus_tag="PKZ52_00770"
     CDS             complement(<1..2346)
                     /gene="secA"
                     /locus_tag="PKZ52_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNG58324.1"
                     /translation="MIGNLLKKIIGTKNDRELKRMRKVVDQINALEASVSSLSDEELK
                     AKSAEFRAQHQAGKSLDAILPEAFAVCREASKRIMGMRHFDVQLIGGITLHEGRIAEM
                     RTGEGKTLMATLAIYLNAVSGRGVHVVTVNDYLARRDAEWMRPLYEGLGLSVGVILSM
                     QPQWEKREAYTADITYGTNNEYGFDYLRDNMVMRKEERVQRPLNFAVVDEVDSILIDE
                     ARTPLIISGAAEDSSELYRIMNSFIPELKPKVEGAEGTEGAGHYVVDEKTRHIELTEE
                     GHELVEQILAKRGLLAEGQSLYAAGNLNMLHHVHSALKAHKLFHRDVEYILQDNQVIL
                     IDEHTGRTMPGRRLSEGLHQAIEAKEGVTIQNESQTLASTTFQNYFRLYPKLSGMTGT
                     ADTESVEFRQIYNLDVVVIPTNKPIARKDLNDLIYLNMEDKFEAIVAEVKRLKDLNVP
                     VLVGTASVETSEEMSARFKAAGIKHEVLNAKQHEREADIIAQAGRPGAVTVATNMAGR
                     GTDIMLGGNCEAELAKLENPSEAEVARIRAEWKARHEQVLAAGGLHILGTERHESRRI
                     DNQLRGRAGRQGDPGVSRFYLSLEDNLMRIFASDRVRAIMNKLGMEKGEAIEHRMVTG
                     AIENAQRKVEARNFDYRKQLLEYDDVANDQRRVIYQQRNDLLDADTIEDAIAGIREDV
                     INDVISNYIPPQSMAEQWDIPALEQSLLADFGVEVPIAQWLKEDAKLYEEPLRRKIID
                     LMNSFYEEKAGRLASVIRGLERQVMLHVLDTLWKEHLLNMDH"
     gene            complement(2459..3367)
                     /locus_tag="PKZ52_00775"
     CDS             complement(2459..3367)
                     /locus_tag="PKZ52_00775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNG58325.1"
                     /translation="MKFILINHKRGRTYSVTFGFWAKALLALCGIGLPCGVAALLVLN
                     LSQVSRFLAQPEAAWTDPLSTALEARDQQAKVNALAMRVAELQAKLMRLDALGERISS
                     NAQLDLKEFDFSQAPGLGGPVSGQPARVYALSELNSLVDQLSARLESRQHQLEYLDEW
                     VSQQKFASDALVQGAPVKDGYRSSEFGWRTDPFTGQSEMHEGVDFSAAEGSEIAAVAS
                     GLVTWSAEKTGYGNLVEINHGNGFKTRYGHCKKNLVHLGDMVQKGQVIALLGNTGRSS
                     GPHVHFEVYKNNRVVDPASYIRTTVP"
     gene            complement(3567..4478)
                     /gene="lpxC"
                     /locus_tag="PKZ52_00780"
     CDS             complement(3567..4478)
                     /gene="lpxC"
                     /locus_tag="PKZ52_00780"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNG58326.1"
                     /translation="MIRQRTLKNAIRATGVGLHTGKQVTLTLRPAPIDHGIVFRRTDL
                     SPVVTIPAKAHFVGDTTLSTTLIKDGVRVSTVEHLLSAMAGLGIDNAIIELNADEVPI
                     MDGSAGPFVFLIESAGIQEQRAAKKFIRILKPVTVQDGDKTASFLPYEGFKVSFGIDF
                     DHPVFRDHKKAVSVDFSNASFVKEISRARTFGFMHDIEMLRAKGLAKGGSIDNAIVID
                     AGGIMNKDGLRYEDEFVKHKVLDAIGDLYLLGFGIIGEYSAFKSGHGLNNKSLRELLS
                     RTDAWEYVTCEDTAKAPISYIKPLIAA"
     gene            complement(4676..5860)
                     /gene="ftsZ"
                     /locus_tag="PKZ52_00785"
     CDS             complement(4676..5860)
                     /gene="ftsZ"
                     /locus_tag="PKZ52_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNG58327.1"
                     /translation="MFELVDNLPQNAVIKVVGVGGGGGNAVKHMINNHVEGVDFVCAN
                     TDAQALKNIEAHAVLQLGNNMTKGLGAGANPDVGRQAALEDRDRIAEVLRGADMVFIT
                     AGMGGGTGTGGAPVVAEVARELGILTVAVVTKPFPFEGRKRMAIAEEGIKHLQERVDS
                     LITIPNEKLLAVLGKTTSLLDAFKAANDVLLGAVQGIADLIIRPGMINVDFADVRTVM
                     SEMGMAMMGTGAARGDNRAREAAEAAIRSPLLEDVNLQGAKGILVNITAGMDLSLGEF
                     TLVGDTIEEFASADATVVVGTVIDPEMKDELRVTVVATGLGLVAKPSAATKAPDPIRA
                     QPQQAEVPKPAYAGAGIERPQGPLRAKQSTISVAQALDMREKDMDYLDIPAFLRRQSE
                     SQ"
     gene            complement(5922..7151)
                     /gene="ftsA"
                     /locus_tag="PKZ52_00790"
     CDS             complement(5922..7151)
                     /gene="ftsA"
                     /locus_tag="PKZ52_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNG58328.1"
                     /translation="MAQANGSKMIVGLDIGTSKIVAIVGEISPEGELSVIGLGSHKST
                     GLKKGVVVNIDSTVQAIQRAIEEAELMAGCQIHSVFAGIAGSHIRSLNSHGIVAIKDK
                     EVLNQDLERVIDAAQAVAIPADQKILHILPQEYLIDEQDGVKDPLGMSGVRLEAKVHL
                     VTCAINAAQNIEKCIRRCNLEVEDIILEQLASSYAVLTDDEKELGVCMVDIGGGTTDI
                     AIFTEGAIRHTGVIPIAGDQVTNDIAMALRTPTQYAEAIKIKYACALATLTNPDEMIQ
                     VPSVGDRPPRSLSRQSLAEVVEPRYDELFTLVQAELRRSGFENLIPAGIVLTGGTSKM
                     EGVIELAEEIFDMPVRLGIPQNVRGMRDIVTNPIYSTGVGLLLYGMKQKGMHKVASFG
                     PRESSWNRLKGWLARNF"
     gene            complement(7178..8080)
                     /locus_tag="PKZ52_00795"
     CDS             complement(7178..8080)
                     /locus_tag="PKZ52_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNG58329.1"
                     /translation="MMDVRRREVPPRRARRHSGQLGASPLRTSKPARQWSFRWLKPLL
                     LWLVYGGLFSGIVASIIWLDPKTRLENLMNRPIAGVSVESEFKYIAQQQTQQLVEQHL
                     SPTFLELDMLGLKNALEANPWIDKVIIARTWPDRLLVRIQEQKPIARWGTEGFINMRG
                     EIVRVADAAQLTSLPVIDCEERYAAEVMQQYQVIKNIVAGNGFTPVRLTLDKTLAWTL
                     EFSPALTIRLGREKTLEKLQNIAKILRTELSREVSRMSQIDMRYDNGFAVTWKDAPPA
                     EPLATSPAAPAANKPEQTPSSAEE"
     gene            complement(8077..9048)
                     /locus_tag="PKZ52_00800"
     CDS             complement(8077..9048)
                     /locus_tag="PKZ52_00800"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNG58330.1"
                     /translation="MNKFSTVALDFFADPNDSAALVRVLGRVAVVYGGTSAEREISLH
                     SGQAVIAALQAEGVDVIGVDIRQNPIAQLQAVKADRVFIALHGPGGEDGRMQALLEYL
                     QLPYTGSGVAASALCMDKQRTKQVWQACGLPTPEYAVLTNDTDWAAVFAQLGPEIIVK
                     PIHEGSSIGMMRVNSLEGLQQAFENAQQFDKGVLAERFIKGAEFTVALLNGVTLPPIG
                     LKPAEAFYDYHAKYFAEDTQYLCPCGLPLEAEARLKSLAAQAFAAVGAAGWGRVDVMA
                     DSAGNFYLLEVNTVPGMTSHSLVPMAAKAAGLSFNQLVLAITSQTLP"
     gene            complement(9061..10485)
                     /gene="murC"
                     /locus_tag="PKZ52_00805"
     CDS             complement(9061..10485)
                     /gene="murC"
                     /locus_tag="PKZ52_00805"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNG58331.1"
                     /translation="MADFPIHQVPEMRRIKRIHFIGIGGAGMSGIAEVLLNQGYSISG
                     SDLKISDVTKRLSAMGMQVFIGHAASNLKDVDVVVRSTAVNESNPELEAARAARIPIV
                     RRAEMLAELMRYRHGIAVAGTHGKTTTTSLIASILAAAGLDPTFVIGGLLNSAGTNAR
                     LGESRFLVAEADESDASFLHLQPMVSVVTNIDADHMETYGGSFSKLQQTFVEFLHNLP
                     FYGLAVMCGDDPVIKELLPQISRSVITYGFGADCDYQALDVVQTGRSQVFKIRCPGIA
                     EPVSISLNLPGRHNVLNATAAAAVAMEEGVSLAAIQKGLAEFSGVGRRFQVYGEFSLG
                     QGEPVMLVDDYGHHPREVAATIAAVRAGWPKRRLVMVYQPHRYTRTRDLFDDFAQVLS
                     SVDQLVLLEVYSAGEDKIEGADSRTLARSIRNRGSVDPVFVEQVDGVLDVLKPLLRPD
                     DIVITQGAGNVGSLAAILANNLMQ"
     gene            complement(10478..11548)
                     /gene="murG"
                     /locus_tag="PKZ52_00810"
     CDS             complement(10478..11548)
                     /gene="murG"
                     /locus_tag="PKZ52_00810"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNG58332.1"
                     /translation="MSQRYLIMAGGTGGHVFPALAVAKALMAQGHQVEWLGTVRGLEA
                     KVVPSAGIQLHCLNIAGVRGRGIRQLLLAPWLIVKSVFTVRQILRRFKPAVVIGLGGY
                     VAGPGGLAAFLSRIPLLIHEQNAVAGTTNRLLARLARRVLCAFPKVLPGAINVGNPLR
                     LNFYQQADPAQRFNQRDGAPRLLVLGGSLGAQALNQMVPKALALLPVEARPLVRHQTG
                     SQHAAGCELAYQERQLNARVEPFIDDVAEALAWADLVICRAGALTVAELSAVGVGAIL
                     VPYPHAIDDHQTANAQWLVAQQAALLAPQESLNAERLSRLLTELLPDRARLCAMATAA
                     RAAATPDATEAFVQHILELTHG"
     gene            complement(11545..12744)
                     /gene="ftsW"
                     /locus_tag="PKZ52_00815"
     CDS             complement(11545..12744)
                     /gene="ftsW"
                     /locus_tag="PKZ52_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNG58333.1"
                     /translation="MGPAAVIAPNAHRSLGPGQSPTARLRPRPVAASPQGLINWPLLI
                     LVLALISFGVVMVASASMDFAAKTYGDPWYFAKRHLLFLALGTAGAIVVFCIPSEIWN
                     RYAIGLLFLGLFLLVVVLVPGIGKSVNGSRRWLGLGGFGIQASEVAKFCFVVFFASFL
                     ARRTEEFQHSWSAFFKLIGILGAFVLLLLVEPDFGTSVVLSITAGAMMFVAGVPLFRF
                     IFLAASGVAGLAMIAIASPYRWQRLVTFLDPWAEQFSSGYQLVQSLIAFGRGSWTGLG
                     LGNSLQKLFFLPEAHTDFIFAIIAEEFGLLGAIVLIAAFVGLVWKVLAVSFRARAQDR
                     SFVCFASFGIGILLAAQSFINMGVASGLLPTKGLTLPFISSGGSSLIVCCAFLALVLR
                     MDAEMRR"
     gene            complement(12726..14105)
                     /gene="murD"
                     /locus_tag="PKZ52_00820"
     CDS             complement(12726..14105)
                     /gene="murD"
                     /locus_tag="PKZ52_00820"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNG58334.1"
                     /translation="MSLIASSHTTLVLGLGATGLSVARYLSRSGRPFAVADSRSAPPN
                     LAAFQAEFPSITPHLGALSAQLLCQFSQIILSPGIPREEPAVAAAIAAGVPVIGDIDL
                     FVQEAAAPIVAITGSNGKTTVTTLMGEMAKACGLDVGVGGNLGVPALDLLSPSRELYV
                     LELSSFQLESTRQLGAHAACILNLSDDHMDRYSAVPGCRSPMQAYHRAKQRIYFGAKH
                     IVANKEDPLTQPPLAEGVKCVQFSLSQPDLHDYGLLQEGAEVFLAKGLTPLLNTCELH
                     IRGRHNWANALAALALGEAAGLKREQMLATLKNFNGLPHRCEFVASKNGVDYFNDSKA
                     TNVGATLAAIAGMANRPRQIVLILGGDGKGADFSPLVKACQHSPTGESPVKAVVTLGR
                     DGPLLAQLVRPHLPVVEASDLAEVVRQAAALAEAGDLVLLSPACASLDMFKSYEARGQ
                     QFAEWVRQL"
     gene            complement(14155..15237)
                     /gene="mraY"
                     /locus_tag="PKZ52_00825"
     CDS             complement(14155..15237)
                     /gene="mraY"
                     /locus_tag="PKZ52_00825"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNG58335.1"
                     /translation="MLLWLAQQFEAQLHILGVVQYLTLRAILGVLTALGISLLAGPWF
                     IRTMESLQMGQSVRTDGPKSHLSKSGTPTMGGTLILFAICVSLLLWADLTNRFVWALL
                     FILVVYGLVGWVDDYRKVIEKNSKGLPAKWKYLWQSLGALAVGIYLYSTASLPSETQL
                     YVPFFKNVALDLGVFYILLVYLVVVGASNAVNLTDGLDGLAIMPTVMVGSALGIIAYL
                     VGNAKFALYLHIPYVPGTGELVVFCSALAGAGLGFLWFNTYPAQVFMGDVGALALGGA
                     LGLVAVMVRHEIVFFIMSGIFVLETVSVILQVASFKLTGKRIFRMAPIHHHFELKGWP
                     EPKVIVRFWIITVILVLFGLATLKLR"
     gene            complement(15238..16647)
                     /gene="murF"
                     /locus_tag="PKZ52_00830"
     CDS             complement(15238..16647)
                     /gene="murF"
                     /locus_tag="PKZ52_00830"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNG58336.1"
                     /translation="MMNLTSWAAAAQGTLVGENRSVSSVCTDSRTLQAGDVFFALKGP
                     NFDGHAYLAQVAAAGAAAAVVEQLDPSLGLPQILVADTVAALGKIASRWRQEHFHGTL
                     FAITGSCGKTTVKGMLKSICEQAGATLATAGNLNNHLGVPLTLMQLRPPHQFAVIEMG
                     ASGRGEIAYLTALARPDVALVNNVQPAHVEGFGSLAAIREEKLRIFAGLAENGTLVLN
                     LDDNYVREAYDEIAAYGQNKIGFSLQPSVPAGAPACRLSQALQAHSLPNGAWGFEWAS
                     GETHLPIELSVLGKHNIANALAAASMALAAGIAPAAIAAGLALFGGEKGRMQAKIAPT
                     GAILIDDTYNANPGSVAAAIAYLAERPRPRILVLGDMKELGQDARAQHRNIGQLAQAA
                     GVDQLYTVGDLSAAATEAFGPGAEHFPTQAALIAALKPVLNRATSCLIKGSRSARMEH
                     IVEGLLALDATPETQPERD"
     gene            complement(16648..>17218)
                     /locus_tag="PKZ52_00835"
     CDS             complement(16648..>17218)
                     /locus_tag="PKZ52_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNG58337.1"
                     /translation="LASLTTVLALHPEAWSLLPTLTASLQPVTGRMHLISSPHAAPKV
                     VVDYAHTPDALEKALAAVRVHATGKLWVVFGCGGDRDKGKRVLMAQAAEAGADRVVIT
                     SDNPRSEDPHAILADITQGLAHVPWLVESDRRAAIAAAIAAADVDDWVLLAGKGHETY
                     QLVGEQIIPMSDIAEAQAALALYTPSETL"
BASE COUNT         3834 a         5035 c         4779 g         3570 t
ORIGIN      
        1 gtgatccata ttcaacaggt gctctttcca aagggtatcg agtacatgca gcatcacctg
       61 gcgctcaagg ccgcgaatca cagaggctaa acgcccggct ttttcctcat agaagctgtt
      121 catcaggtcg ataattttgc ggcgcaacgg ctcttcatag agcttggcat cttctttaag
      181 ccattgggcg atgggcactt ccaccccaaa atccgccagc aggctctgct ccagcgcagg
      241 aatatcccac tgctcggcca tggactgggg cgggatgtaa ttggagatca catcgttgat
      301 gacatcttcg cgaatgcccg caatggcatc ttcaatggta tcggcatcca acagatcatt
      361 gcgctgctgg tagattaccc ggcgctggtc attggccacg tcatcgtatt cgagcaactg
      421 tttgcggtag tcgaagttac gagcttccac cttgcgctgg gcgttttcga tggcgccggt
      481 gaccatgcgg tgctcaatgg cctcgccctt ctccattccc agtttattca taatggcgcg
      541 cacccggtcg gaggcgaaga tgcgcattaa gttgtcctca agggacaggt aaaaacgcga
      601 taccccaggg tccccctggc gcccggcgcg accgcgcaac tggttatcga tgcggcggga
      661 ttcgtggcgc tcggtgccga gaatatgtag gccacctgcg gccagcactt gttcgtggcg
      721 cgccttccac tcggcgcgga tgcgggcaac ctccgcctca gaagggttct caagcttggc
      781 taattcagcc tcacagttcc cgccgagcat aatgtcagta ccgcgaccgg ccatgttggt
      841 agccacggtc acagcgccag gccggcccgc ctgtgcgatg atatccgctt cgcgctcgtg
      901 ctgtttggcg ttgagcactt cgtgtttgat gcctgcagcc ttaaaccgcg ccgacatttc
      961 ctcggaggtt tccaccgaag ccgtacccac caacacgggg acattcaagt ctttgaggcg
     1021 ttttacctcc gccactatgg cctcaaattt atcttccata ttgaggtaga taagatcatt
     1081 gaggtcttta cgggcgatgg gtttattggt ggggatgacc accacgtcca gattgtaaat
     1141 ctgccggaat tcaacagatt cggtatcggc tgtaccggtc atgccggaga gtttcgggta
     1201 gaggcggaaa tagttttgaa aggtggtact ggccagagtc tggctttcat tctgaatagt
     1261 cacgccttct ttggcttcga tggcctggtg caaaccttcc gataaacgac gaccgggcat
     1321 ggtgcggccg gtgtgctcat cgatcaggat cacctggtta tcctgcagaa tgtactcaac
     1381 atctcgatgg aataatttat gggccttcag ggctgagtgc acatggtgca acatgttcaa
     1441 attgcccgct gcgtacaggc tctggccctc tgccaacagg ccgcgcttgg cgagaatttg
     1501 ctccaccagc tcgtggcctt cttcggtcag ctcaatgtgg cgggtttttt catccaccac
     1561 ataatggccc gccccttcag tgccttctgc gccttcaacc ttaggcttaa gctcagggat
     1621 aaagctattc atgatgcggt acaactctga ggagtcctcc gccgcgccgg aaataatcaa
     1681 cggcgtgcga gcctcgtcga tcaggataga gtccacctca tccaccaccg caaaattcag
     1741 tggtcgctgt acccgctcct ctttgcgcat caccatgttg tcgcgcaggt agtcaaagcc
     1801 gtactcattg ttggtgccgt aggtgatgtc tgccgtatag gcttcgcgtt tctcccattg
     1861 gggctgcatg gacaaaatca cccctacact caggcctaag ccttcataca gcgggcgcat
     1921 ccattcggca tctcggcggg ctaagtaatc attgacggta accacatgca caccacgacc
     1981 tgaaacggca ttgagataaa tagccagggt cgccatcagg gttttaccct caccggtgcg
     2041 catctccgca atgcgcccct cgtgcagggt aataccgccg atcaactgca catcaaaatg
     2101 gcgcatgccc ataatgcgtt tactggcctc tcggcaaacg gcaaacgcct cgggcaggat
     2161 cgcatctagg gacttacccg cctggtgttg agcgcgaaac tcggcgcttt ttgccttcag
     2221 ttcctcatcg ctgagggagc tgacactagc ctctaacgca ttaatctgat cgacgacttt
     2281 gcgcatccgc ttcagttcgc gatcattctt agttccaata atttttttca gtaaattgcc
     2341 aatcataaaa tcgccatcaa gccacccagc cggggggcgg catagtaaaa gaaagtctat
     2401 ttaagggaaa tacacacagc agccattaca ggccgctttt agaaagcgtg gaatagagtt
     2461 agggaacagt ggtgcggata taagaggctg ggtcaacaac gcggttgttt ttatacactt
     2521 caaagtgtac gtgggggcca gaggagcggc cggtattgcc cagcagagca atcacctggc
     2581 ccttctgcac catatcgcca aggtgcacaa gatttttctt gcaatgccca tagcgagttt
     2641 taaagccatt gccgtggttg atttccacca aattaccgta gccagttttt tccgccgacc
     2701 aggtgacaag ccctgatgcc accgccgcta tttcactgcc ctcggcagcg gaaaagtcca
     2761 cgccctcgtg catttcggac tgaccggtaa aagggtcagt gcgccagcca aattctgagg
     2821 aacgataacc gtctttaacg ggtgcccctt gcactaaagc atcactggcg aacttctgct
     2881 gcgacaccca ttcatccagg tactcaagct ggtgttgacg gctttcgagc cgtgcactga
     2941 gctgatcaac caagctatta agctcgctaa gcgcatacac acgcgcaggc tgcccactca
     3001 cagggccgcc gaggcccggg gcctgactga agtcgaactc ttttaaatct aattgtgcat
     3061 tgctgctgat gcgctcaccc agtgcatcta ggcgcatcaa cttcgcctgc aactcggcca
     3121 cccgcatggc caaggcattc accttagctt gttgatcgcg ggcttcaagc gcagtgctta
     3181 ggggatcagt ccaagccgcc tccggctggg cgagaaaacg cgacacttgg gataggttca
     3241 acaccagtaa agccgccaca ccacagggca gcccgatacc acacagggcc agtaaagcct
     3301 tggcccagaa cccgaacgtc acactgtaag tgcggcctcg tttgtggttg attaatatga
     3361 acttcacgcc caaatcatcc tgctatctat tgtggttatt actggagcta gcgcttttgg
     3421 aggcgcggaa tatggcacag gcggcggcct ggttcaatag aataggtcat ggcacggcgc
     3481 tatttcggaa aaatctccac gaggcacacg aattttgggc gaaaaaaaac ccggcatagc
     3541 cgggctttgc aactattgaa tgcaacttaa gcggcaatca atggcttgat gtaagaaatg
     3601 ggcgccttgg cggtatcttc acaggtgaca tattcccagg cgtcggtacg gctgagcaat
     3661 tcgcgcaaag atttgttgtt aaggccgtga ccggacttaa acgcgctgta ctcaccgata
     3721 ataccgaagc ccaacaggta caaatcaccg atggcatcaa gcaccttgtg cttcacgaac
     3781 tcatcttcat accgcaaacc gtctttgttc atgatgcctc ctgcatcaat gacaatggcg
     3841 ttatcaatgc tgccgccttt agccaggcct ttggcacgca gcatttcgat atcgtgcata
     3901 aaaccaaagg tgcgggcgcg cgaaatttcc tttacaaaag acgcgttgga aaaatccacc
     3961 gatacggcct ttttgtggtc gcgaaataca gggtggtcga agtcaatacc aaagctcacc
     4021 ttaaaccctt catagggtaa gaagcttgca gtcttatcgc catcctgaac cgtgaccggc
     4081 ttgaggatac gaataaactt cttggctgcg cgctgctctt ggataccagc ggattcaatc
     4141 aagaatacaa atggcccagc gctgccatcc atgataggca cttcgtcagc attcaattca
     4201 atgatggcgt tatcaatgcc caaacccgcc atagcggaga gcaagtgctc aacagtggaa
     4261 acacgcacac cgtctttgat cagggtagtg gacagcgtgg tatccccaac aaaatgggcc
     4321 ttagccggaa tggtgacaac aggagaaaga tcagtgcggc gaaacactat gccgtggtca
     4381 atcggcgcag gacgcagggt cagcgtcact tgcttaccgg tatgcaaacc caccccggtg
     4441 gctcgaatgg cgttttttaa ggtgcgctgt ctgatcatct gtatctacct gtgcgaatac
     4501 cgcaatggct aaaggaataa atcgaggcta tctcgatact tcacagattc tagtctggcc
     4561 agatgaatcc aagctgaact ttcaggttca gttttcgcac ctaagctgct gatcccacac
     4621 aaaaaaagcc ccattaaggg gctttttcac aggcatacgg ctggcgctgc aaggcttatt
     4681 gagactcgga ctggcggcgc aaaaaggccg gaatatccaa atagtccata tccttctcgc
     4741 gcatatccaa ggcttgcgcc acagaaatgg tactctgttt ggcacgcaac gggccctgag
     4801 gacgctctat accggcacct gcgtaagcgg gcttgggtac ttctgcctgc tggggctggg
     4861 cgcgaattgg atcgggagcc ttggtggcgg cgctgggttt ggcaactaag ccaagaccgg
     4921 tcgccacgac agtaacccgc aactcgtctt tcatctccgg atcaataacg gtacctacca
     4981 ccacagtggc atcagcggag gcaaattctt cgatggtgtc gcccaccagg gtgaactcgc
     5041 ccaaggacaa gtccataccc gccgtgatat tgaccaaaat acccttagcg ccctgtaaat
     5101 taacatcttc caacagcggg ctgcggatag cggcttcggc ggcttcgcgg gcgcggttat
     5161 cgccacgggc ggcgccggtg cccatcatcg ccatgcccat ttccgacata acagtgcgca
     5221 cgtcggcaaa gtccacgttg atcatacctg ggcgaataat taaatccgcg atgccctgaa
     5281 ccgcgcccaa caaaacatcg ttagcagctt taaaggcatc gagcagactg gtagttttgc
     5341 ccagtacggc cagtaatttt tcgttcggaa tggtgattaa agaatccacg cgctcctgca
     5401 aatgtttgat gccttcctcg gctattgcca tgcgcttgcg gccttcaaat ggaaaaggct
     5461 tagtcactac ggcaacggtt aaaatgccca gttcgcgggc tacttctgcc accacgggag
     5521 caccgccggt gccggtgcca ccgcccatac cggcggtgat aaacaccatg tccgcaccgc
     5581 gcagcacttc agcgatgcgg tcgcgatctt ccaaagcggc ttggcggccc acatcggggt
     5641 tggcgcctgc gcctaaacct ttggtcatgt tgttgcccag ctgcaaaacc gcatgggctt
     5701 ctatattttt cagcgcttgg gcgtccgtat tggcgcagac gaaatctaca ccttctacgt
     5761 gattgttaat catgtgtttg accgcgttgc cgccgccacc accgacaccc actactttaa
     5821 ttaccgcgtt ttgcggcaag ttatcgacta attcaaacat tttcagctcc ccagatatcc
     5881 cctgaatttg ccattgtact caattgcttg cttggatggg attaaaaatt gcgcgctaac
     5941 caacctttta ggcgattcca tgaactctcg cgcggaccaa atgaggcaac cttatgcatg
     6001 cctttctgtt tcatcccata caacaacaat ccaaccccag tggaatatat ggggttggtg
     6061 actatatcgc gcataccgcg cacgttttga ggtataccca aacgcaccgg catatcaaaa
     6121 atttcctcag caagctcaat aaccccttcc attttagagg tgccgccggt cagtactatt
     6181 cctgccggga tcaggttttc aaagccgctg cggcgcagct cggcttgtac gagggtaaag
     6241 agttcgtcat agcgcggctc gaccacctcg gccagcgatt ggcgcgatag gctgcggggc
     6301 ggtcgatcgc cgacactggg tacctgaatc atttcgtccg ggttagtgag tgtggccagg
     6361 gcgcaggcgt atttgatttt gatggcttcg gcgtattgcg tcggggtgcg cagggccatg
     6421 gcgatgtcat tggtaacctg gtcaccggct atgggaatta cccccgtatg gcgaatggcc
     6481 ccttcggtga agatggcaat atccgtggtg ccgccgccaa tatccaccat gcacacgccc
     6541 agctcttttt catcgtcggt gagcacggcg tagctggagg cgagctgctc cagaatgatg
     6601 tcttccactt ccagattaca gcggcggata catttttcga tgttttgggc ggcgttgatg
     6661 gcacaggtca ccaaatgcac cttggcctct aagcgcacgc cggacatacc taatgggtct
     6721 ttaacgccgt cttgctcgtc gatcaaatat tcctggggca gaatatgcaa gattttttga
     6781 tcggcgggga tggccaccgc ttgggcggcg tctataaccc gctctaaatc ctggttcaaa
     6841 acttctttat ctttgatcgc gactataccg tgggagttga ggctgcggat atgggagcct
     6901 gctatgcctg caaacaccga gtgaatttgg cagcccgcca tcaattctgc ttcttctatg
     6961 gcgcgctgga tggcctgtac cgtggagtcg atgttcacga ccacgccttt tttaaggccg
     7021 gtggacttat gggagccgag gcctatgaca gacagctctc cttccggtga aatctccccg
     7081 acaatggcga cgattttgga tgtccctata tccaggccga cgatcatttt gctcccatta
     7141 gcttgagcca tgttttactc cagcgcctgt atgcgctcta ttcttctgcg gatgatggtg
     7201 tttgttctgg tttgttggca gccggtgccg cagggctggt ggccagaggc tccgcaggcg
     7261 gggcatcttt ccaggttacg gcaaagccat tgtcataacg catgtcaatc tggctcatac
     7321 gcgagacttc acgggataat tccgtgcgca aaattttggc tatattttgc aatttttcta
     7381 gggttttttc tcgccctaag cgaatggtta gcgctggcga gaattctagg gtccaggcga
     7441 gggttttgtc taaagtcagc cgcaccggtg taaagccgtt gcccgctact atatttttga
     7501 tcacctggta ctgctgcatc acttccgccg catagcgctc ttcgcaatcg attaccggca
     7561 gcgaggtcaa ttgcgcggca tctgctacgc ggacgatttc cccgcgcatg ttgataaagc
     7621 cctcagtgcc ccagcgggca atcggttttt gctcttgaat gcgcaccaac aaccgatcgg
     7681 gccaggtgcg ggcgataata accttgtcga tccagggatt ggcttccaaa gcgtttttaa
     7741 ggcccagcat atccagctct aaaaaggtcg gcgacagatg ttgctccacc aactgctggg
     7801 tttgctgttg ggcgatgtac ttaaattcgc tctccacact gacaccggca atgggccgat
     7861 tcatgaggtt ttccagccgc gttttaggat ccagccaaat aatgctggcc actatgccgg
     7921 agaacagacc gccgtacacg agccagagta aaagcggttt gagccagcgg aagctccact
     7981 ggcgcgcagg tttagaggtg cgcagaggcg aggcgcccag ctgcccagag tggcgtctgg
     8041 cgcggcgagg tggcacttca cggcggcgca catccatcat ggaagggttt gactcgtaat
     8101 ggcgagcacc aactgattaa aacttaagcc cgccgctttt gcggccatgg gcaccaggct
     8161 gtggctggtc atgcccggca cagtgttcac ctctaacagg taaaaattgc ccgcgctatc
     8221 ggccatcaca tccacccgcc cccaacctgc ggcgccgacg gcggcaaagg cttgggcggc
     8281 aaggctctta aggcgcgcct cggcttccag gggtaggccg caggggcaga ggtattgggt
     8341 atcctcggca aaatatttgg cgtgatagtc gtagaaggcc tcagcgggct taaggcctat
     8401 gggcggtaag gtgacaccgt ttaacagcgc cacggtgaat tcagcgcctt tgataaaacg
     8461 ctcggccaat acacccttgt caaattgctg ggcattttca aaggcctgct gcaggccttc
     8521 cagactattg acccgcatca tgccgatgct ggagccttcg tgaatcggtt tcacgatgat
     8581 ctccggcccg agttgggcaa atacagcggc ccagtcggta tcattcgtca gcaccgcgta
     8641 ctcgggagtg ggcaggccgc aggcttgcca gacttgtttg gtgcgctgtt tgtccataca
     8701 cagggcggaa gcggcaacac cgctgccggt ataaggcagc tgcaaatatt ccagcagcgc
     8761 ctgcatgcgg ccgtcttcac cgccggggcc atgcagggcg ataaacacgc ggtcggcctt
     8821 taccgcttgt aattgtgcga tggggttttg gcggatatcg acgccaatta catcgacacc
     8881 ttcggcttgc agcgcggcga ttaccgcctg gccgctgtgc agggaaatct cccgctcggc
     8941 cgaggtgccg ccgtacacca ccgccactcg ccccagcacc cgcactaagg cggcgctgtc
     9001 gttggggtcg gcgaagaaat ccaaggctac agtggaaaat ttattcatag cgaatacaca
     9061 tcattgcatc aaattattag ccaatatggc ggcgaggctg cccacgttgc cagcgccctg
     9121 ggtgatgaca atatcatcgg gccgcaacag gggtttgagc acatccaaaa caccgtccac
     9181 ctgctcgaca aacaccgggt ctacactgcc gcgattgcga atggagcggg ccaaggtgcg
     9241 cgagtcagca ccttcgattt tgtcctcccc ggcgctgtat acctccagca acaccaactg
     9301 atccacgctg gataacacct gggcgaagtc gtcgaacaga tcccgggtgc gggtatagcg
     9361 atggggctgg tacaccatca ccaagcgacg cttgggccaa ccggcccgca ctgcagcgat
     9421 ggtggctgcc acttcgcggg ggtgatggcc ataatcatcc accagcatca caggctcgcc
     9481 ctggcctagg gaaaactcac catacacctg gaagcgccgc cccacccctg aaaattccgc
     9541 caaacctttt tggatggcgg cgagacttac gccctcctcc atggccaccg cagctgcggc
     9601 cgtggcattg agcacattat ggcggccagg cagattcagg ctgatggaga caggttcggc
     9661 aattcctggg cagcggattt taaagacttg gctgcggcct gtctgcacta cgtcgagggc
     9721 ttggtagtcg caatcagcgc cgaaaccgta ggtgatcacc gagcgggaaa tctgcggcag
     9781 caattcttta atcaccggat cgtcgccaca catcaccgcc aggccataaa acggcaggtt
     9841 gtgcagaaat tccacgaagg tttgctgcag tttgctgaag ctgccgccgt aggtttccat
     9901 atgatcggca tctatgttgg tgacgaccga caccatgggc tgcaaatgca ggaacgaagc
     9961 atcgctctcg tccgcctcgg ccaccaaaaa ccggctctca cctaagcgcg cgttggtgcc
    10021 agcgctgttg agcaggccgc cgatgacaaa agtggggtca agccccgccg cggcgaggat
    10081 ggaggcaatc aagctggtcg tggtggtttt gccgtgggtg ccggcaacgg cgatgccgtg
    10141 gcggtagcgc atcaactcag cgagcatttc cgcccgccgc actataggaa tccgcgcggc
    10201 gcgggcggct tccagttccg ggttgctctc attcacagcg gtggagcgca ccaccacgtc
    10261 tacgtcttta aggttgctgg ctgcgtggcc gataaacacc tgcatgccca tggccgacaa
    10321 tcgcttagtg acatccgaga ttttcaggtc ggaaccggaa atgctgtagc cctggttgag
    10381 cagcacctcc gcaatgccgc tcataccggc gccgccgata ccaataaaat gaatgcgttt
    10441 gatgcggcgc atttcaggca cctgatgaat aggaaaatca gccatgggtt agctccagaa
    10501 tgtgctgcac gaacgcttcg gtggcatccg gcgtggccgc cgcccgcgcc gctgtggcca
    10561 tggcgcacag gcgggctcga tcgggcaaaa gttcagttaa taaccggctt aagcgctcgg
    10621 cgttgaggga ttcttggggc gccaatagcg ccgcttgctg tgctaccaac cactgggcgt
    10681 tggcggtttg gtgatcgtcg atggcatggg ggtatggcac taagatcgcg cccacgccca
    10741 cggcgctgag ctccgccacg gtgagggcac cggcgcggca gattaccagg tccgcccagg
    10801 ccagggcctc cgccacatca tcaataaagg gttctaccct cgcgttgagt tggcgctctt
    10861 ggtaagccag ctcacaaccg gccgcgtgct ggctgccggt ttggtggcgc accaggggcc
    10921 gcgcctccac cggcaacagc gccagggcct ttggcaccat ttggttgagc gcctgggcgc
    10981 cgaggctgcc gccaagcacc agtagccgtg gcgcgccatc gcgctggtta aagcgctggg
    11041 ctgggtccgc ttgttgataa aaattcagcc gcagaggatt gccgacatta atggcgccag
    11101 gcaaaacctt aggaaacgca cacaacaccc ggcgggccag gcgcgcgagc aaacggttgg
    11161 tagtgcccgc caccgcgttt tgttcatgaa tgagcagcgg aatgcggctt aaaaatgccg
    11221 ccaggccacc ggggcctgcc acataaccgc ccaggccaat caccacggcg ggtttaaagc
    11281 ggcgcagtat ctgccgcacg gtaaataccg atttcacaat cagccagggc gccaataaca
    11341 gctggcggat gccgcggccg cgcaccccgg cgatgttcaa acaatgcagt tgaatgcccg
    11401 cgctgggcac aactttagcc tccagccccc gcaccgtgcc cagccattcc acctgatggc
    11461 cctgggccat gagcgccttg gccaccgcga gggcgggaaa tacatggccg ccggtgccgc
    11521 ccgccataat caaatagcgc tggctcatcg gcgcatctcc gcgtccatac gcagcaccag
    11581 ggccaaaaaa gcgcagcaga caatcaggct gctgccgccg gagctaataa acggcagggt
    11641 gaggcctttg gtgggcaaaa gccctgaggc cacccccata ttaataaagc tctgggcggc
    11701 caggagtatg ccgatgccga agctggcaaa acagacaaac gagcgatcct gggcgcgagc
    11761 gcgaaaactt acggcgagga ctttccaaac caggccgaca aaagcggcaa tgagcacaat
    11821 ggcgccgagt agaccaaatt cctcagcgat aatggcaaag atgaagtccg tatgggcctc
    11881 cggcaaaaag aacagctttt gcaggctatt gcccagtccc aatcccgtcc aactgccgcg
    11941 cccaaaggca attagtgact gcaccagctg ataaccgctg gagaactgct ccgcccaagg
    12001 gtctaaaaag gtcaccagcc gctgccagcg atagggcgag gcgatggcga tcatggcaag
    12061 gccggcaacc ccggaggcgg cgaggaaaat aaagcgaaac aagggcaccc cggccacaaa
    12121 catcatggcc cccgcagtga tgctcagcac tacggaggtg ccgaaatccg gttcgaccaa
    12181 aagtaacaac acaaaggcgc ccaggatgcc gatgagctta aaaaaagcgc tccaggaatg
    12241 ttgaaattcc tcggtgcggc gggccaaaaa gctggcaaaa aacaccacaa aacagaactt
    12301 cgccacctcc gacgcctgaa tgccaaagcc gcccaatccc aaccagcgac gggagccatt
    12361 aacactttta cctatgcccg gcaccaatac cactaccaat aaaaacagac ccagaaataa
    12421 cagacctatg gcatagcgat tccagatctc gctgggaata cagaacacca ctatggcgcc
    12481 cgcagtcccc agggccaaaa acagtagatg gcgtttggca aagtaccagg ggtcgccata
    12541 ggttttagcg gcgaagtcca tggaggcgga cgccaccatc accacgccaa aggagatcag
    12601 cgccaagacc aaaatcagca atggccaatt gatcaagccc tgagggcttg cggccacagg
    12661 ccgcgggcgc aggcgcgcgg tggggctttg gccggggccg agactgcggt gggcgttggg
    12721 ggcaatcaca gctgccggac ccattcggca aactgttgac cgcgcgcttc gtagctttta
    12781 aacatatcca ggctggcgca ggccggcgat aacaacacca ggtcaccagc ctccgccagg
    12841 gcagcggcct gtcgcaccac ttcggccaag tcggacgcct ctaccaccgg cagatggggg
    12901 cgcactaact gcgccagcag cgggccatcg cggcctaaag tcaccaccgc cttcaccggg
    12961 ctttcacccg ttggcgagtg ttggcaggct tttaccagcg gcgaaaaatc tgcccctttg
    13021 ccgtcgccac cgaggatcag cacaatctgc cgggggcggt tggccatacc ggcgatggcg
    13081 gccagggtcg ctcccacgtt ggtggctttg gaatcattaa aatagtccac accgttttta
    13141 cttgcgacaa actcacagcg gtggggcaag ccattaaagt ttttaagggt ggcgagcatc
    13201 tgctcgcgct tgaggccagc cgcctccccc agggccaggg ccgccagggc attggcccag
    13261 ttgtggcggc cgcgaatgtg cagctcacag gtgttgagca gtggtgttag ccccttggcg
    13321 aggaatacct ccgccccctc ctgcagcagg ccataatcgt gtaaatccgg ctggctgagg
    13381 ctgaactgca cacacttcac cccttcggcc agaggcggct gggtgagtgg gtcttcttta
    13441 ttggcgacta tgtgtttcgc gccgaaatag atgcgctgct tggcgcggtg ataggcctgc
    13501 atggggcttc tacagccggg caccgcactg tagcgatcca tatgatcgtc gctgagattc
    13561 aaaatacagg cggcgtgggc gcccaactgc cgagtgctct ccagctggaa gctcgatagc
    13621 tccagcacat agagctcccg gctggggctt aataaatcca gggcgggcac acccagattg
    13681 ccgccgacgc ccacatccag gccacaggct ttggccattt cgcccatcag ggtggtgacg
    13741 gtggttttac cgttggaacc ggtgatggcg acgatgggcg cggccgcttc ctgcacgaat
    13801 aggtctatat cgccgatcac cggcacaccc gccgcgatcg ccgccgctac cgcaggctcc
    13861 tcccgaggga tgccggggct gaggataatt tggctgaact ggcacagcag ctgggcggat
    13921 agggccccta ggtgtggcgt aatgctgggg aattcggcct gaaatgcggc cagattaggt
    13981 ggcgccgagc gcgaatcggc cactgcaaag gggcgcccgc tgcggctgag gtagcgcgcc
    14041 accgaaagcc ccgtggcgcc taggcccaac acgagggtgg tgtgagagga ggcaattagg
    14101 ctcatagcag ggcgacttct cggctcatta cggggcggta catcggttta ttcatcagcg
    14161 cagttttagg gtcgcaaggc caaagagtac aaggatcacg gtaataatcc aaaaacgcac
    14221 aatcaccttg ggttcgggcc agccttttag ctcaaagtgg tggtgaatgg gcgccatgcg
    14281 gaaaatgcgc ttgccggtga gcttaaaaga cgccacctgc aaaatcaccg agacggtctc
    14341 aagcacaaaa atgccggaca taataaaaaa cacaatctca tggcgcacca tcacggccac
    14401 aagccccaag gcaccgccga gggctagggc gcctacatcg cccataaata cctgagccgg
    14461 ataggtgtta aaccacaaaa agccaagccc ggccccggcc agagccgagc agaacaccac
    14521 cagctcaccg gtgcccggca cataggggat atgcaaatag agggcgaact tagcgttacc
    14581 caccaggtag gcgatgatgc ccagggccga gcccaccatc actgtaggca taatggccag
    14641 gccatctaaa ccgtcggtga ggttcactgc attgctcgcc cccaccacta ccaggtacac
    14701 cagcaggata taaaacactc ctaagtccag agccacattc ttaaaaaatg gcacgtacag
    14761 ctgggtttcc gacggcaggc tcgccgtgct gtagagataa atacccactg ccaaagcgcc
    14821 caacgactgc cacaggtact tccacttggc gggtaggccc ttggagtttt tttcaatcac
    14881 cttacggtaa tcgtccaccc aacccaccag gccatacacc accaggataa acagcagcgc
    14941 ccacacaaag cggttggtta aatccgccca caacaacaaa ctgacacaga tggcaaataa
    15001 aatcagggtg ccgcccatgg tgggcgtgcc ggatttactc aagtggctct tggggccgtc
    15061 ggtgcgcacg gactggccca tttgtaagct ctccatggtg cggataaacc aggggccggc
    15121 cagcagcgag atacccaggg cggtcaacac acccaagatg gcccgcaggg tgaggtattg
    15181 caccacaccc aaaatatgta attgcgcctc aaattgctgg gcgagccaaa gtaacatcta
    15241 atcgcgctcc ggttgagttt caggggttgc atcgagggcc aaaagtccct cgactatatg
    15301 ttccatacgc gcactgcggg agccctttat caggcaactg gtggcgcgat tgagtacagg
    15361 ttttaaggcg gcaattaagg ccgcttgggt ggggaaatgc tcagcgccgg ggccaaaggc
    15421 ttcggttgcg gcagcgctta aatcccccac ggtgtagagc tgatcaacac ccgccgcttg
    15481 agccaactgg ccgatgttgc ggtgctgcgc ccgtgcatcc tgccccagct ctttcatatc
    15541 ccccagcacc aagatacgcg gcctcgggcg ctcagccagg tacgcgatgg cggccgccac
    15601 cgaaccgggg ttggcattgt aggtatcatc aatgaggatt gcacctgttg gcgcaatttt
    15661 cgcttgcatg cggccctttt ccccgccaaa gagcgccaaa cccgctgcaa tagccgcagg
    15721 agcaatcccg gcggccaagg ccatactggc ggcggccagg gcgttggcaa tattgtgttt
    15781 gcccagcaca ctaagctcta tgggcaggtg ggtttctccg ctggcccact caaaccccca
    15841 ggcgccattg ggcaagctgt gcgcctgcag cgcctggctt aagcggcagg ccggagcacc
    15901 tgcaggcact gatggctgca ggctaaagcc gattttattc tgcccatagg ccgctatctc
    15961 gtcataggcc tcacgcacgt agttatcatc cagattcaaa accagggtgc cgttctcagc
    16021 gagccccgca aaaatgcgca gtttctcttc acggatagcc gccagggagc caaaaccctc
    16081 cacatgggcg ggctgtacat tgttgacgag ggccacatcc ggcctggcca gggcggtgag
    16141 gtaggcgatt tcgccgcggc cgctggcgcc catttcgatc accgcaaatt ggtggggtgg
    16201 ccgcagctgc atgagggtca gcggcacccc caggtgattg ttcagattac ccgccgtggc
    16261 aagggtggcg cccgcctgct cgcagatact ttttaacatg ccttttaccg tggttttgcc
    16321 gcagctgccg gtaatggcaa acaaggtgcc gtgaaaatgc tcctgccgcc aacgactggc
    16381 gattttgccc agggcggcca ctgtgtcggc gaccaaaatt tgcggcagcc caaggcttgg
    16441 atccaactgt tccaccaccg ccgccgccgc ccctgccgcc gccacctggg ctaagtaggc
    16501 atggccatca aagttagggc cttttaaggc aaaaaacaca tcccccgcct ggagtgtgcg
    16561 actgtcggta cagacgctgc tgaccgaccg gttttcacct actaaggtgc cctgggcggc
    16621 cgcagcccaa ctggttaaat tcatcattta gagcgtctcg ctgggggtat aaagcgccag
    16681 ggccgcctgg gcctcggcta tatcactcat ggggataatc tgctcgccaa ccagctgata
    16741 ggtttcatgg cctttgcccg ccagcaacac ccaatcatca acatcggcag cggcaatggc
    16801 ggcggcaatg gcggccctgc gatccgactc cacaagccaa ggcacgtgtg caagcccctg
    16861 ggttatgtcg gcaagaatgg catgggggtc ttcgctgcga gggttatcac tggtgataac
    16921 cactcgatcg gcgccagctt cagctgcctg cgccatcagc acgcgcttac ccttatcgcg
    16981 gtcgccgccg caaccaaata ccacccacag cttgccagtt gcgtgcaccc gtacggcggc
    17041 aagggctttc tctagtgcgt ccggagtatg ggcgtaatcc actaccactt tgggtgctgc
    17101 atgcgggcta ctaatgaggt gcatgcggcc tgtaaccggc tgcaggctgg ccgtaagggt
    17161 tggcaagagt gaccaggcct caggatgcaa ggccaatact gtggtcaggc tcgccagc
//
LOCUS       DAOQGS010000010        16553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1600_length_16553_cov_4.955752, whole genome shotgun sequence.
ACCESSION   DAOQGS010000010 DAOQGS010000000
VERSION     DAOQGS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16553
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_16553_cov_4.955752"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1309
                     /locus_tag="PKZ52_00840"
     CDS             425..1309
                     /locus_tag="PKZ52_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58338.1"
                     /translation="MSNPVKRRVSNPRTFRVGIVGASVEAMNAFARIFSVTNFRNRIY
                     EAVPLMTNSRRLPDDVDFVILCTLNPTVLAAWDRGLIEGPAAARPQVRLSRRGSTQGE
                     QSPAGYFLESPINPSRLIKLLDTYTIKELNYLPEFEIGGDSTRLCEDAVAGLKLLNRP
                     EAIAAARREGLNKALVVDDSLAVRRQLEIEFGLLGVEVTSVDTAEAALGAALGCKFDM
                     IFLDVVLPSMDGYAACRRIKKIQANKNTPIILLTSKSSSFDRIKGALAGCDTYLVKPI
                     NHSDFERIINDQFPQPKE"
     gene            1314..1676
                     /locus_tag="PKZ52_00845"
     CDS             1314..1676
                     /locus_tag="PKZ52_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58339.1"
                     /translation="MTASKIMVVDDSAVDLQYLKLIIEGAGYQVVTASSGREAVEKAR
                     STRPDLILMDVVMEGGDGYEACRAITSDRETGKIPVIFVTSKSQKADRVWAELQGGRG
                     LISKPYSAEEIIRTIAAI"
     gene            1685..2155
                     /locus_tag="PKZ52_00850"
     CDS             1685..2155
                     /locus_tag="PKZ52_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNG58340.1"
                     /translation="MALGVPQVNPAQHVTLGARAYGFTVGGQGYMVSPGVYSELIKNP
                     SWSALPNVQHHFAGLLNVRGTIVPVFHLDCFINGTTPATAAPYVLVIGTLAQAAALVL
                     NEKPQAIDLSRSVQTDDYLHAPKAIAHCIKHSYRCDNQVWLELSHEVLFSTLAG"
     gene            2415..4478
                     /locus_tag="PKZ52_00855"
     CDS             2415..4478
                     /locus_tag="PKZ52_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG58341.1"
                     /translation="MNVFSSLRVSQKILLLVAWLALGFAALGVAYYWQVDQEAKVRET
                     AQNYSLFEKFLQQGRGHLQDFIANSYLGAASEELKASQEAFISTFNHAQLLAKKEPSL
                     PNPQTIVSAFEALQAASTPGGEAAPIQQRYAEEKIRTQFKILEAIVKQRAKPQELAAW
                     VELQRLHEGFMADRQEVSLASLQGFVRDFSTTQKSVLRPDAGLVHLLAALDDYAASFT
                     PEPVPAQQAGFDGRKYQPVLQQVVQLQQGLESQLIKFTASMDEQIQLVRAIFIALIFV
                     VVMGAAVGIYFIYKSIVFPLVHMQNVMQRINKGNSKARVKILEQDELGDLGRAFNQLL
                     DDRFQFLQDQFLENEILNNSIISLIRAVGLIARKDLTIHVPVSADITGTISDAINLLT
                     SETAKTLWQVKDISAQVNELADALQKQSRLVVQVAEDERKQVIATGKAMEISAKTMGD
                     IAAKAQNADKFAVMAIDNTQSARDTVSRSVSGILSIRETISETEKRIKRLGDRSQEIS
                     GIVNLINTIAERTHILALNASMHAASAGEAGKGFAVVADEVQRLAENAREATAEISSM
                     VNNIRIETADAVNTMNKLISQVAEGTRLAESAGAKMEDTVAVTRRLVEQVQLIAHSST
                     QQAELSNRVRDRATIIRSFTEKTGLQLTQQKQHTDSLKAFCETLVERVNSFTLPEHDK
                     EHSSV"
     gene            4560..7379
                     /locus_tag="PKZ52_00860"
     CDS             4560..7379
                     /locus_tag="PKZ52_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58342.1"
                     /translation="MTTSQPTAEQQELIGLLHSELSDLASYDLSPLHSGDEEAKNALL
                     DELFSHLDNLGHAARLVGLLGVERVCEHLKANFLIWQHQPWVDENSILMAGWCPLLIH
                     YLTSIGSEQENTAVSQLVEYVLDNRWPQGEHYEALQQAFSSSEIIADEDSPISQLPSF
                     VDEDMVSLAIDSDVNRDLLNGLLQELPEHSASFSRAVARLVEEQDTAALAIALRAAHT
                     VKGAANLVGIRGLANLMHYTEDLLELIVKQSGAVAQASVDLLEDTADALASISEYLIG
                     LGPKPAQLIELLSRVLTQLRQAYSGQWEYSSQALIPGVSSVQEPAPVLVDEQDTDTHQ
                     HHLTVTETMAQELLRIAGENQIGTNQVLTRIGQLVAGVKSAESYHQKIRALAASFDHL
                     VQLHGIKNTANIERGGDFDPLEMERFSELHSFTSQLQEVTTDSQEAIYHIEAQLKELT
                     NLVIEQRHRSSDAQNLLLHIRMLPVSLIAPRLARCVRQAARLTQKNVRFELQGEGVLV
                     DSRVLNTVVDPLMHLLRNAVDHGIESAPRRAELGKDSEGLIRVSFNRRGENLSIVVED
                     DGAGLDYARIEGLARERGILELYENPTRAELHKILLTPGFSTRHQVSQTSGRGIGLDV
                     VATQLRALKGSMSIQSALNRGTSFALEMPMSILSAHTLVIKMGSHKCSLVSRGVEQLI
                     YILPTDINWAEEKPQYIYNGETLDVLRIDDLAMMPGQNAVARYSALLITRRGEHGRVA
                     IAVESILASEDHIIKPLNKYSCKPAGVVGAAILGDGSVSPVLDLHEFPTLQMNDQEYQ
                     RWLRVKDEYQARFVPSSPKLVALVVDDSLSARRALAQFMTDLGLDVQTAKDGFEAIAV
                     MEKIKPDIVLVDLEMPRMNGLELTNHIRAGETDEHLPIVMVTSRVTDKHRQMAAQVGV
                     DAYLNKPWTEEDLMRLVQEQLPEMA"
     gene            complement(7482..9458)
                     /locus_tag="PKZ52_00865"
     CDS             complement(7482..9458)
                     /locus_tag="PKZ52_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HNG58343.1"
                     /translation="MFCFSPKALSLALAASFLCHTLNAQSATSSDVIDAVPEKSLEPI
                     VIDPLPVPIKIGTSTLSCTLSASSSTSSTYEISYKVANTGKNTVFNWQVQLPFNQAPR
                     IIKVANAETRGSPTVTASNIASNGTLKPGQATVFTLAGYHNGYFVPPSCIPYPPSNSS
                     SSSSSSSSSSSSSSSSSSSGSSSSSSSSSSSSSSSSGGVVINIDRSLFVHDQATLSAS
                     NITLSRVMDRLASQLSAANPSDTITGSTLFARLWDAQNPQGSQPATGIAQCNGLVNNW
                     PIDCRPVEGGQSRDPLTFINQYIPIALVNRFDLRDQASFTDCGEYRVIFANPISSRNF
                     IIFEAQVPNPTPGTASGCLPIQNLWAGLSSNNSAASRGAILEDFYFNGLPNANVRPVI
                     DSRNYAQNTGQIRTNQFMTGPWLLKEYKAGIQNGKNTIQVVSVKSNPVGRLFEVTNAD
                     PRAVSFRSDFVQNLPSLLDPNLATFFISVTNDAHNNGESHSQIPGGNDFLNHFAVSRG
                     SSFEAAISSRIVQAGSNLTVDQVLNRATAMTCGGCHQPSTFGIGSVGAVGPGQGWPDS
                     AGFVHVNEFASSGVFPLSPALTQVFLPARRTDMAAFLNASTAKVSLPIKVIPGITPAP
                     TPITPVIDAQELQLNSQNTTDNATSTTTRSKRAG"
     gene            9746..10834
                     /locus_tag="PKZ52_00870"
     CDS             9746..10834
                     /locus_tag="PKZ52_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNG58344.1"
                     /translation="MAEQAQGAVVHFVTGGFSGATQVARTLVAQANRSGVRTHLLVLR
                     RKRQTRQAQLDELTAQQIPFFVVPGWSHLATIIALVKLLKRIAPRVLIAHGFSEHLWG
                     RIAAIIAGVPVIIHVEHNSRERYTPLRLWLAKQLTKHTRWLVGVSEGVKNSLLALGFA
                     PEKCVFVNNGIDAERFARLPVVEFSQRKNAVIMCARFARQKDHETLIRAVAQLNHMGV
                     KIELALLGGGKSSLQKRAEQLVQSLGINEQVTFLGHSNQVPELLATYQIAALITHYEG
                     MPLALVEAMAAGCAVVASNVVGVKEILALGIGVAVEAKAVDQLAQVLADLLQNPIKAE
                     QIGNAARAYALEHFTQAKMLAGYEALIK"
     gene            complement(10937..11932)
                     /locus_tag="PKZ52_00875"
     CDS             complement(10937..11932)
                     /locus_tag="PKZ52_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNG58345.1"
                     /translation="MSPEPLPTPWLSILIPVYNVQDYLEACLQSVLSQADSGVEVIAL
                     DDVSTDNSLALLKQIQAQSSHPIRIIQHANNQGLSAARNSMLTEACGDYIWFLDSDDI
                     ICPGSLAALKTIIQNHTPDLVLCDFYMLRENTQLKHKLRGELHRKTFGGSENQLSSDK
                     NALFYHLYKTAQLHIWSKIAKRSLWGDDLRFPSGQLMEDQTVTPRLFLRANTYYYCPQ
                     VWIGYRQRPGSILSTPNRQRLIDSSISAKDVLKEWLQKHPDLTDQSRFTFAYTRAKMF
                     IGIARILRRSQQINDLNWHRQQFFDNSQCNLNWLIMQYIKRGWFLRLARLLYHLR"
     gene            complement(12013..15039)
                     /locus_tag="PKZ52_00880"
     CDS             complement(12013..15039)
                     /locus_tag="PKZ52_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNG58346.1"
                     /translation="MLPALTTPGLDALTEKFLHTLAALGFKGDIHSDYASRTVLATDN
                     SIYQVLPQAVLYPKDQQDLVYILELAANTTFQTLVIAPRGGGTGTNGQSLTPGVVVDM
                     SKHMNQILAIDPVARTARVQAGVVKDQLNAALKPHGLFFAPELSTSNRATLGGMINTD
                     ASGQGSVLYGKTRDHVISLTSVLLGGQVWQSQAMDEATLSEVCSRTDKIGQIHQLLQS
                     INHEQGALIKAKFPPLNRCLTGYDLAHIRDDEGRFNLNNILCGSEGTLGFISEATLNC
                     LPIPKHTALVVVTYPDFNAALRHANALMSASPASIETVDSTVLNLAMQDNAWQEVAEF
                     FPSSGSLVQGINLVEFIGDELEALEANLAAFAQRLDTEQQEKNACSGYSFVRGAGSVN
                     KIWGMRKKAVGLLGNAAGEARPVPFVEDTAVPPEHLADYIAEFRALLDARGLRYGMFG
                     HVDAGVLHVRPALDLKDPQQAQQIRAISDEVAALTYKYGGLLWGEHGKGVRSEYAPQF
                     FGELYPQLQRIKAAFDPHNQLNPGKIATPSAHLALLKIDEVSMRGEFDRQIGVQTWQG
                     FSEAVYCNGNGACYNWAANDPMCPSWKVSRDRVQSPKGRASLIREWLRQMAREGADPT
                     ALLAKPRHWLKEIIELPLRLRNRRRDTTDFSHQVKASMNTCLACKSCVGQCPIKVDVP
                     EFRSKFLALYHLRYPRPLKDYLVGSLEFILPWAAKVPRLYNGLMAFGPIKTLLSRVVG
                     FEDSPKLSGINLAQALSAQGVALATPQAIAALSPEQKARAVVLVQDAFTSYFETQVVI
                     DAALLLQKLGFYPLLAPFQANGKPLHVHGFLRAFKAVAARQAQTLNSLAQAQLPLVGI
                     DPAMTLCFRSEYSKYLDGQMPQVQLLQEFLHARRSEFGEIKCVADKSFKLFPHCTEKT
                     NAPSSTGQWQQVFKTLGLQLDVVATGCCGMSGTFGHESANLASSKALFAMSWQAPLHE
                     HGAQALVTGYSCRSQSLRQMGVKPLHPVQGLLQSLA"
     gene            15298..>16553
                     /locus_tag="PKZ52_00885"
     CDS             15298..>16553
                     /locus_tag="PKZ52_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58347.1"
                     /translation="MQSFLVQLRRPLWAVLVAAALLLGFLSHSLVIQLHNFSIKSAAG
                     EPSPINVPFLKQGTGAMDYVVKGELTLGWLAPSLYNVIPDDLLLKLVINGQEVDLSDQ
                     PYERLKDVNQGVTLNLGRFLHTGSNSIEVHFRDFSGDMGLTIKTSPLDVRVLGLFIAW
                     VLVASIAAVALLQCFGLSRVRSVLYWLIAAGSVIRVAYIFTYNPLDHIFSDPQRHWEQ
                     GTEVLRRDLMALTDPIGYQIYVGALAKLSVKLPELVAFYTSLLALLGAWFWYRFLREL
                     QGSKTLALAGWAFLSLLPSWTAIYAYFMQETLFIVLLGAALWATWRCRRKADAASFAA
                     MVFFWTAAGLTRGIAIPLAAVACTWLWFLQAGKVRKALYSSAILLFILGPLTARTYPL
                     VHHFAPHGMGQLNVIYAQSGKKVIEV"
BASE COUNT         3755 a         3971 c         4739 g         4088 t
ORIGIN      
        1 gagtaaccaa cataaacagc tatttggccg cgaccgctca accgccgcag tgcccgcagc
       61 ctgtgaactg tctgcagggg aagcgtatgc ccaactgggc gagtgcgggc ttagctgcgg
      121 gtaagaatgg tgcagtgatt cgcgcagaac ttcaataact gtttgaatgg taaataaata
      181 ttcgcggcgc gcctattatc ttgtagactt tgcccggcaa aaatttccgg cctgaactag
      241 gctggggttg aaggtggccg agttaaataa tctggatgtg ttactagagc gcttgatgct
      301 tgccttgtta ggcgtgtcgg ggagcgcctg gcaactgttt tgagttgtag tctgccatac
      361 agggggtgtg gtttctctgg cacagggttg gagtgcttca ctaccacgaa aattttggtt
      421 gatcatgtca aatcccgtaa aacgcagagt ttccaatccg cgcacctttc gcgttggcat
      481 agtgggcgcc tcggtggagg ccatgaatgc cttcgcacgc atctttagcg tcaccaattt
      541 tcgcaaccgc atttacgaag ccgtcccttt aatgaccaac agccgccgcc taccggacga
      601 tgtggacttt gttatcttat gtacccttaa ccccacggtg cttgccgcct gggatcgcgg
      661 cctcattgag gggcctgccg cagcccgccc ccaagtgcgc ctaagccgcc gcggcagcac
      721 gcagggggag caaagcccgg cgggttattt tttagaatcc cccatcaatc ccagccgcct
      781 aattaaattg ctcgacacct acacgattaa agagctgaat tacctgccgg aatttgaaat
      841 cggcggcgac agtacccgct tgtgcgaaga tgccgtcgcc gggctgaagt tgttaaatcg
      901 gccggaggcg attgccgccg cccgccggga gggcttaaat aaagccttgg tggtggatga
      961 tagcctcgcc gtgcggcggc aattagaaat tgaatttggc cttttgggtg ttgaagtcac
     1021 ctcggtggat acggccgagg ccgccttggg tgcagcgcta ggctgcaaat ttgatatgat
     1081 ttttttggac gtggttttac ccagcatgga tggctatgcc gcctgtcgcc gcattaaaaa
     1141 aattcaggcc aataaaaata ccccgattat tttactcacc agtaaatcct cttcatttga
     1201 ccgtattaaa ggcgcattag cggggtgtga cacctatctg gttaagccta ttaatcactc
     1261 cgattttgag cgaattatta acgaccaatt tccacagccg aaggaataaa tctatgacag
     1321 ccagtaaaat catggtggta gacgactcag ccgtagattt gcaatatctt aaattaataa
     1381 ttgaaggtgc cggttaccaa gtagtaaccg cctccagcgg gcgcgaagcc gtggaaaaag
     1441 cccgctccac acggccggat ttaatcttaa tggatgtggt gatggagggc ggcgacggct
     1501 atgaagcctg tcgcgcaatc acctccgacc gcgaaaccgg caaaatcccc gttatatttg
     1561 ttaccagcaa aagccaaaaa gctgaccgtg tatgggccga attgcaaggt gggcgcgggc
     1621 ttatttctaa accctattcg gcggaagaaa ttatccgcac gattgccgca atttaagttg
     1681 ttttatggct cttggtgtac cccaggttaa tcccgctcag catgttactt taggcgcacg
     1741 ggcctatggg tttaccgtgg gcggccaggg ttatatggtg tcgccgggtg tatacagcga
     1801 attaattaaa aatcccagct ggtcggcgct acccaatgtg caacaccatt ttgccggcct
     1861 attaaatgtg cgcggcacaa ttgtgccggt atttcatttg gattgtttta ttaatggtac
     1921 taccccggct actgcggcgc cctatgtgtt ggtgattggc accctggccc aggcggccgc
     1981 tttggtgttg aatgaaaaac cccaggcaat tgatttaagc cgtagtgtgc aaaccgatga
     2041 ttatttgcat gcgccgaaag cgattgccca ttgtataaag catagttacc gctgcgataa
     2101 tcaagtctgg ctggaattaa gtcatgaggt gttgttttcc accttggccg gttaatagcc
     2161 cggtgagcgt gtaggccgcc aaaatacaga gtgttttgtc agaataatta gttttttatc
     2221 agaataatta agggagcatc ggatcgattg gggttgttaa aatccgccgt aagctgcccg
     2281 cctgcgccgt ttttgagaac agatcatcct gcagaatgct cttaaataag ggaccgctaa
     2341 ttaggtgatg tatttgaaca tccacttaat tacagtttcc taaaagtgct gaacgtaacc
     2401 taaggttaga tcctatgaat gtgttctcaa gtttgcgtgt atcgcagaaa attctgctgt
     2461 tagtggcctg gctggcgtta ggttttgccg ctttgggggt cgcttattat tggcaggtgg
     2521 atcaagaagc caaggtgcgg gaaaccgcgc agaattacag tttatttgag aagtttttgc
     2581 agcaggggcg cgggcattta caggacttta ttgccaattc ttatttgggg gctgccagcg
     2641 aagagcttaa ggccagtcaa gaggcattta tcagcacatt taatcacgcc cagctccttg
     2701 cgaaaaaaga accctcgctg cccaatccgc aaactatagt cagcgctttt gaggcgctgc
     2761 aagcggcctc gacgccaggc ggggaagctg ctcccattca acagcgctac gcagaagaaa
     2821 aaattcgcac acaatttaaa atactagagg cgattgtaaa gcagcgcgcc aaacctcaag
     2881 agcttgccgc ctgggtggaa ttacaacgct tacacgaggg ttttatggcg gaccggcagg
     2941 aggttagcct ggccagtttg caaggcttcg tgcgcgattt tagtaccacg caaaaaagtg
     3001 ttttgcgccc ggatgcggga ttggtgcatc tattagccgc tttggatgac tatgccgcaa
     3061 gctttacccc ggagcctgtg cctgctcagc aggcagggtt tgacgggcgc aagtaccaac
     3121 cggttttgca gcaggttgta caattgcagc aggggctaga gagtcaatta attaaattta
     3181 ccgccagcat ggatgagcaa attcaattag tgcgcgccat ttttattgca ctgatttttg
     3241 tggtggtgat gggcgctgcg gtgggtattt atttcatcta taaaagcatt gtattcccgc
     3301 tggtgcatat gcaaaacgta atgcagcgta ttaacaaggg taactcaaag gcgcgggtaa
     3361 aaattctgga acaggatgag ctgggtgact taggccgcgc ttttaaccaa ttattggatg
     3421 atcgcttcca gtttttgcag gatcaatttt tggaaaatga aatcctcaat aactccatta
     3481 tttctttaat tcgcgccgtg ggtttaatcg cccgtaaaga tttaaccatt cacgtacctg
     3541 tatcggcgga tattaccggt actatttccg atgcgattaa ccttttaacc tcggaaacgg
     3601 caaaaaccct gtggcaggtg aaagatattt ccgcccaggt taatgagctt gccgatgccc
     3661 tgcaaaaaca atcccgcctg gtggtgcagg tggccgaaga tgagcgcaag caggtaattg
     3721 ccaccggtaa agctatggag atttccgcta aaaccatggg cgatatcgct gccaaagcgc
     3781 aaaacgccga taaatttgcg gtaatggcca tcgataatac ccagtctgcc cgcgatacgg
     3841 taagccgctc ggtgagcggt attttaagta ttcgcgaaac catttcggaa acggaaaaac
     3901 ggattaaacg tttgggggat cgctcccagg agatttccgg tattgtaaat ttgattaata
     3961 ccatcgccga gcgcacccat attttggcct taaacgccag tatgcacgcg gcgtctgcag
     4021 gtgaagcggg caaaggtttt gcggtggtgg ccgatgaggt gcagcgcttg gctgaaaacg
     4081 cccgggaggc cacggcggag atttcctcga tggtaaacaa tattcgcatc gaaacggccg
     4141 atgcggttaa taccatgaat aaattgattt cccaagtagc cgagggaacg cgcttggcag
     4201 aatccgcagg cgccaaaatg gaagatacgg tggcggtgac gcggcgcttg gtggagcagg
     4261 tacagcttat tgcccacagt tcaactcagc aggcggaact gtccaaccga gtgcgggata
     4321 gagcgactat tatccgcagt tttacggaaa aaaccggcct gcagcttacc cagcaaaaac
     4381 agcataccga ttccttaaaa gcgttttgtg aaactctggt tgagcgggta aacagcttca
     4441 cgctgcctga gcacgacaaa gaacattcgt ctgtataaat cagcttcgcg gtgcgagcgc
     4501 gaaggctttt gtcccgcacc agccctgcaa tgtttattgc gcaattggag catgcatcta
     4561 tgacgacgtc ccagcctacg gccgaacagc aagagttaat tgggcttttg cattctgagc
     4621 tgtctgattt agcttcctac gatttatctc ctttacacag cggtgatgaa gaagctaaaa
     4681 acgctttgtt agatgagtta tttagccatt tggataactt aggtcacgcc gcgcgcctgg
     4741 tgggtttgtt gggggtggag cgcgtatgcg agcatttaaa agcgaatttt ttaatttggc
     4801 aacatcagcc gtgggtggat gaaaattcaa tattaatggc cggctggtgc ccgctgttga
     4861 ttcactacct caccagtatt ggcagcgaac aggaaaatac cgctgtaagc cagttggtag
     4921 aatatgtgtt ggataatcgc tggccccagg gggaacacta cgaagcatta cagcaggcgt
     4981 tttccagttc agaaattatc gccgatgaag acagccccat cagtcaattg ccgagttttg
     5041 ttgacgagga tatggtgagc ctcgccatcg acagcgatgt caaccgcgat ttattaaacg
     5101 gcttattgca agaattacct gaacactcgg ccagtttctc ccgcgccgta gcgcgcttag
     5161 ttgaagagca agataccgct gccctggcca tcgccttgcg agcggctcac accgtaaaag
     5221 gcgccgccaa tttggtgggt attcgcgggc ttgcgaattt aatgcactac acagaagatc
     5281 ttttagaatt aattgttaaa caatctgggg cggtggcaca ggcatcggtg gatttattgg
     5341 aggataccgc cgatgccttg gcgagtattt ccgaatattt aattggccta gggcctaagc
     5401 ctgcgcagtt aattgaattg ttaagccgcg tattaaccca gttgcggcag gcttattccg
     5461 gccagtggga atacagcagc caggctctaa ttcccggtgt tagcagcgtg caggagcctg
     5521 ccccggttct cgtggatgaa caggatacag acacgcatca acatcattta accgtcaccg
     5581 agaccatggc gcaggagtta ttgcgcatcg ctggggaaaa ccaaatcggt actaaccagg
     5641 tattaacgcg tattggccag ttggtggcgg gggttaaatc ggcggagtcc taccatcaaa
     5701 aaattcgtgc ccttgcggcc agtttcgatc acttggtgca attacacggc attaaaaaca
     5761 ccgctaatat cgagcgcggc ggcgattttg accccttgga aatggagcgt ttcagcgagc
     5821 tgcacagttt taccagccag ttgcaagaag tcactaccga ctcccaagaa gccatttacc
     5881 acattgaggc gcaattaaaa gaacttacca acctggtcat tgagcagcgc caccgcagca
     5941 gcgatgcaca aaacctgttg ttgcatattc gcatgttgcc ggtatcgtta attgcaccgc
     6001 gtttagcgcg ctgtgtgcgc caggcagcgc gcttaacgca aaaaaatgta cgctttgaat
     6061 tgcaggggga aggtgtatta gtggacagcc gcgtgctgaa tacggtggtg gaccctttaa
     6121 tgcacttatt gcgcaacgca gtggatcacg gcattgaaag tgcgccgcgc cgcgctgaat
     6181 tgggcaaaga cagcgaaggt ttaattcgcg tgagctttaa tcgccgcgga gaaaatttaa
     6241 gcatagtggt ggaggacgac ggcgcgggat tagattatgc ccgtattgag gggcttgcac
     6301 gggagcgcgg cattttagag ctctatgaaa atcccacccg cgcagaacta cataaaatac
     6361 tgcttacgcc ggggttttct acccgccatc aggttagcca aacctcgggg cggggcatag
     6421 gtttggatgt ggtggccacc cagttgcggg ccttaaaagg cagtatgagt attcaatcgg
     6481 cgctcaatcg cggcacttcg tttgcgctgg aaatgcccat gagtatttta tctgcccata
     6541 ccctggtaat taaaatgggc agccataaat gttcattggt gagccggggc gtggagcagt
     6601 taatttatat tctgcccacg gatattaatt gggcggagga aaaaccccag tatatttata
     6661 acggtgaaac cctggatgtg ttgcgcattg acgatttggc catgatgccg gggcaaaatg
     6721 ctgtggcgcg ctacagcgct ttattaatta ctcggcgcgg tgaacacggc cgtgtggcca
     6781 ttgcggtgga gagtattctc gccagtgaag accatattat taaaccctta aataaatatt
     6841 cctgtaagcc tgccggtgta gtgggggcgg cgattttggg ggatggttca gtaagcccgg
     6901 tgttggattt acacgagttc cccaccttgc aaatgaatga tcaggaatat cagcgctggc
     6961 tgcgggtaaa agatgagtat caagcccgtt ttgtgcccag ttcgcccaaa ttagtggccc
     7021 tggtggtgga tgattcttta agtgcccgcc gcgctttggc gcaatttatg acggatttag
     7081 gtttggatgt acaaaccgca aaagacggtt ttgaagctat cgccgtgatg gaaaaaataa
     7141 agcccgatat tgtgctagtg gatttggaaa tgccgcgcat gaacggcttg gaattaacca
     7201 atcatattcg cgccggggaa accgatgagc atttgccgat tgtgatggtg acttctcggg
     7261 ttaccgataa acaccggcag atggcggcgc aggttggtgt ggacgcctat ctaaataaac
     7321 cctggacaga agaagattta atgcgtttgg tacaggagca attgccggag atggcttagc
     7381 acaatttatt aacgcggcgg gatttagtgc cgggttaata gttgtgtgct ggataaaaat
     7441 taaaaaggcc tgctgaataa tccagcaggc ccgatagcgc attaaccggc gcgtttgctg
     7501 cgggttgtag ttgaagtggc gttatccgta gtgttttggc tattcagctg cagctcctgc
     7561 gcatctataa ccggcgtaat gggcgttggt gcaggtgtaa tgcccggtat tactttgatc
     7621 ggtaggctca ccttggcggt gctggcattt aaaaacgctg ccatgtcagt gcggcgcgcc
     7681 ggtaaaaata cctgggttaa agccggtgaa agcggaaata cgccgctgct cgcaaattcg
     7741 tttacgtgca caaaccccgc tgaatccggc cagccttgtc ccggccccac ggcgccaacg
     7801 gaaccaatgc caaaggtgct gggctggtgg cagccgccac aggtcatggc ggtggcgcgg
     7861 ttgagcactt gatccacggt taaattactg cccgcttgca caattctact gctgatcgcc
     7921 gcttcaaatg agctgccgcg gctaactgca aaatgattga gaaaatcatt accgccggga
     7981 atttgtgaat gactttcgcc gttattgtgg gcatcgttag tgactgagat aaaaaaggtc
     8041 gccaaattag gatctaacaa gctcggtaag ttttgtacaa aatcgctgcg gaaactcacc
     8101 gctctggggt cggcattggt cacttcgaat aagcggccca ccgggttact ttttacgctg
     8161 acaacctgga tggtattttt accgttttga atgcccgcct tgtattcctt gagtaaccag
     8221 gggcctgtca taaattggtt ggtgcggatt tggccggtat tttgtgcgta gtttctgctg
     8281 tcgattaccg ggcgcacatt ggcattgggc aggccattaa aataaaaatc ctccaaaatg
     8341 gcgccgcggg atgcagcgga attattgcta cttaatccag cccacagatt ttgtatcggc
     8401 aaacagccgc tggccgtgcc gggtgttggg ttggggactt gggcttcaaa aataataaaa
     8461 ttgcgcgagc taatggggtt ggcaaaaatc acccgatatt ccccgcaatc ggtaaagctt
     8521 gcttgatcgc gcagatcaaa acgattgact agcgcgatgg gaatatattg attgataaag
     8581 gtaagtgggt cgcgcgactg gcctccttct acggggcggc agtcgatggg ccaattattc
     8641 accaggccgt tgcactgggc gataccggtg gccggttgtg agccctgcgg gttttgtgcg
     8701 tcccacaggc gggcaaatag ggtgctgccg gtaattgtat cgctggggtt ggcggcgctt
     8761 aattggctgg ccagccgatc cataacgcgg ctgagggtga tgttgctggc gctgagggtg
     8821 gcctgatcat gaacaaataa actgcggtca atattaataa ccaccccacc ggagctggag
     8881 gatgagctac ttgagctgga ggatgagctt gaacttgagc cgctgcttga gcttgagctg
     8941 cttgatgatg aactggagga cgaactagac gagctggatg atgagttaga gggcgggtag
     9001 ggaatacagc taggggggac aaaataacca ttgtggtaac cggcgagggt aaatacggtg
     9061 gcttggccgg gttttaaggt gccgttgctg gcgatattgc tggccgttac cgtggggctg
     9121 ccgcgggttt cagcattggc cacttttata atgcgcggcg cttgattaaa gggcagctgc
     9181 acctgccagt taaaaaccgt atttttgccg gtattggcaa ctttatagct tatttcatag
     9241 gtgctggagg tgctgctact agcggagagc gtgcagctga gtgtgctagt gcctatttta
     9301 atgggcaccg gtaagggatc gatcacaatc ggctctaggg atttttccgg tactgcgtcg
     9361 attacatcgc tactggttgc actctgtgcg tttaatgtgt ggcataaaaa gctggcagcc
     9421 agggctaggc ttaaggcctt tggggaaaaa caaaacatac aaaatcctct ttgtgatggt
     9481 aggttgcgcg cctcggttag cgcgttaaat aacttaacat gtgcttaatc ctgcgtgtgt
     9541 aaaaaagtgt atgtatgagc ggcgtggatt ttattacccc ggcggtaaac ttaatcgtcc
     9601 tgatcgagtt tgccttcgct ccgccggtgc ggcgtccttg ccgccgccat taacccggca
     9661 taaaaagccg ccgggttaat aatgcgctgc ggaaaatgcg tccttaagct acaatcgcgc
     9721 cctttgtttt ttaagcgtgg tgaatatggc ggagcaggcg caaggcgcag tggtacattt
     9781 tgttaccggt ggattttcag gcgctaccca ggtggctcgt accctggtgg cccaagccaa
     9841 tcgcagcggt gtgcgcaccc acctgctggt tctgcgccgc aaacggcaaa cccgccaggc
     9901 gcaattggat gaattaaccg cccagcaaat tccttttttt gtggtgcccg gctggagtca
     9961 tttagcaact attattgctc tggtaaaact gcttaaacgt atagcccctc gtgtattaat
    10021 tgcccatggt tttagcgaac atttatgggg gcgtattgcg gccataattg ccggtgtgcc
    10081 cgttattatt catgtggagc ataattcgcg cgagcgttac acccccctgc gtttatggct
    10141 ggccaaacaa ttaactaaac acacccggtg gttggtgggc gtttctgaag gggtaaaaaa
    10201 tagtttgctg gcgttagggt ttgcgccgga aaaatgtgtg tttgtgaata atggcattga
    10261 tgctgagcgc ttcgcacgat tgcctgtggt ggaattctct cagcgtaaaa atgccgtcat
    10321 catgtgcgcg cgctttgcgc ggcaaaaaga tcacgagaca ttaattcgcg ccgtggcgca
    10381 attaaatcat atgggcgtaa aaattgagtt ggcgctttta ggtggtggta aatcatctct
    10441 acaaaaacgt gccgagcagt tggtgcaaag cctgggcatc aatgagcaag tgactttttt
    10501 agggcattcc aatcaagtgc ccgaattatt ggcgacttat caaattgccg cattaatcac
    10561 ccattatgaa ggtatgccct tagcgttggt ggaggcaatg gcggccggtt gcgcggtggt
    10621 ggcaagcaat gtggtgggtg taaaagaaat attggccttg ggaatagggg tggcggtaga
    10681 ggcaaaagct gttgaccagc ttgcccaggt gttggcggac ttgctgcaaa acccgataaa
    10741 ggctgagcaa ataggcaatg cggcgcgtgc ctatgccctt gagcatttta cccaggcaaa
    10801 aatgctggcc ggttatgagg ccttgattaa ataaagtaat tgctaatttc acaagctctt
    10861 aagcttgcgt cttaatttta tgcaattatt ttaagtgcgt ccgcggactt tattggcgcg
    10921 tcctatggtt gacaccttag cgcagatgat agagcaggcg tgccaagcgc aaaaaccaac
    10981 cgcgttttat gtactgcata atcagccaat ttaaattgca ctggctgttg tcaaaaaatt
    11041 gttggcggtg ccagttgagg tcgttaattt gttggctgcg ccgtaaaatt ctggctatgc
    11101 caataaacat cttggcgcgc gtgtaggcaa aggtaaaccg gctttggtcg gttaaatctg
    11161 ggtgtttttg taaccattcc tttaacacgt ctttggcgga aatggacgag tcgataaggc
    11221 gttgcctgtt gggggtggat aaaatactgc cggggcgctg gcgatagcct atccacactt
    11281 gagggcaata ataataagtg tttgcccgca aaaataaacg cggtgttacg gtttggtcct
    11341 ccatcagttg gccgctgggg aagcgcagat catcgcccca taaactgcgt ttggcgattt
    11401 tgctccaaat gtgcagctgc gcggttttgt ataaatgata aaacagtgcg tttttatcgc
    11461 tgctgagttg attttcactg ccgccaaagg ttttgcgatg caactcgccg cgcagtttat
    11521 gtttaagttg ggtgttctcc cgcagcatat aaaaatcaca cagcactaaa tctggcgtgt
    11581 ggttctgaat aattgttttt aaggcggcga gactgccggg gcaaataata tcgtcggaat
    11641 ctaaaaacca aatatagtcg ccgcaggctt cggtaagcat gctgttgcgg gcagcgctta
    11701 agccctggtt attcgcgtgc tgaataattc gaatggggtg agaggattgg gcttgaatct
    11761 gtttgagcag tgcaagtgag ttatcggtgg agacgtcgtc cagggcaatt acctctacgc
    11821 cgctatccgc ctgggataac accgattgca aacaggcttc taggtaatcc tgcacgttgt
    11881 agacgggaat taaaatactc agccaggggg ttggcagggg ttctggggac ataggctggc
    11941 ctgggggagt agcggattat tggatggatt cggctgcgcc gggggagaaa caccggcggc
    12001 aagctatgaa ttttaagcca ggctctgcaa caagccctgc accgggtgca gcggtttcac
    12061 ccccatctgg cgcaggcttt ggctgcggca ggagtaaccg gtcaccaggg cctgggcgcc
    12121 gtgttcgtgc aggggcgctt gccagctcat ggcgaagagg gctttggagc ttgccaggtt
    12181 agcgctttca tggccgaagg tgccggacat gccgcagcag ccggtggcca ctacatccaa
    12241 ctgcagcccg agggttttaa atacctgctg ccattggccg gttgaactgg gtgcattggt
    12301 tttttccgtg cagtggggaa agagtttgaa gcttttgtcc gctacacatt taatctcgcc
    12361 aaactcgctg cggcgggcgt gcaaaaactc ttgtagcagt tgtacctggg gcatttggcc
    12421 gtctaggtat ttgctgtatt cgctgcgaaa acacagggtc atggctgggt ctatgcccac
    12481 caggggcagc tgcgcttgag ccaggctatt gagggtctgg gcctggcggg cggcgacggc
    12541 tttaaaggcg cgcaaaaagc cgtgtacatg caggggtttg ccattggctt gaaagggcgc
    12601 caagagcgga taaaagccga gtttttgcag cagcagggcg gcatcgatca ccacttgggt
    12661 ttcaaagtaa ctggtgaagg catcctgcac tagcacaaca gcccgggcct tttgctcagg
    12721 gcttaaggcg gctatggctt gcggggtggc aagggctacg ccctgggcac ttaaggcttg
    12781 cgcaaggtta atgccggaaa gcttagggct gtcttcaaaa cccactactc ggctgagcag
    12841 ggtttttata gggccaaagg ccatcaggcc gttgtaaagc ctgggcacct tggccgccca
    12901 gggcaggata aattccaggc tacccaccag ataatcctta agcgggcggg ggtagcgcag
    12961 atggtagagg gccaagaatt tactgcgaaa ctccggtaca tccaccttga tggggcattg
    13021 gcccacgcag gatttacagg ccaggcaggt gttcatgctg gcttttactt ggtgcgaaaa
    13081 atcagttgta tccctgcggc ggttgcgcag gcgcagaggt aattcgatta tttctttgag
    13141 ccaatggcgg ggtttggcca gcagggccgt agggtcagcg ccttcgcgtg ccatctgccg
    13201 cagccactcg cgtattaacg aggcgcggcc cttgggggat tgcacgcggt cgcggctcac
    13261 cttccagctg gggcacatgg ggtcgttggc ggcccagttg taacaggcgc cgttgccgtt
    13321 gcaatacacc gcttcgctaa agccctgcca ggtttgcacg ccaatctgcc ggtcgaattc
    13381 cccgcgcatg ctgacttcat cgattttaag cagggcaagg tgcgcgctgg gggtggcgat
    13441 tttgccgggg ttgagttggt tgtgggggtc gaaggcggct ttaatgcgct gcagctgggg
    13501 gtacagctcg ccgaagaatt gcggcgcgta ttcgctgcgc acgcccttgc cgtgttcccc
    13561 ccagagcagg ccgccgtatt tgtaagtgag ggcggctacc tcatcgctga tggcgcgaat
    13621 ctgttgggcc tgttgcgggt cttttaaatc cagggctggg cgcacgtgca atacacctgc
    13681 gtccacatgg ccgaacatgc cgtagcgcaa accccgtgca tccaacaggg cgcggaactc
    13741 ggcgatgtaa tcggccaggt gctcaggggg cacggcggtg tcctccacaa agggcaccgg
    13801 gcgggcttcg ccagcggcat tgccgagcag gcccacggcc tttttgcgca taccccaaat
    13861 tttgttcacg ctgcccgcgc cgcgcacaaa gctgtagccg ctgcaggcgt ttttctcttg
    13921 ttgttctgta tccaggcgct gggcaaaggc ggcgaggttg gcctccaatg cttccagctc
    13981 atcgccgata aactccacca ggttaatgcc ctgcaccaaa gatccgctgc tggggaaaaa
    14041 ttcggccacc tcctgccagg cgttatcctg catggccagg ttcaacacag tggaatctac
    14101 cgtttcaata ctggcagggc ttgcgctcat tagtgcgttg gcgtggcgca atgcggcgtt
    14161 aaagtccggg taggtgacta ccaccagggc tgtgtgtttg gggatgggca ggcagttgag
    14221 ggtggcttcg ctgataaaac ctaaggtgcc ctcggagccg cagaggatgt tgttaaggtt
    14281 aaagcggccc tcatcatcgc gaatgtgggc caggtcgtag ccggtgaggc agcggtttaa
    14341 gggcgggaat ttggctttga tcagcgcgcc ctgttcgtgg ttaatcgatt gcagcagctg
    14401 gtgaatctgg ccgattttat ccgtgcggct acacacttcg ctaagcgtag cctcatccat
    14461 ggcctgggat tgccatacct ggccgcccaa taacactgag gtgaggctga tcacatggtc
    14521 gcgggtttta ccgtacagca ctgagccttg gccggaggcg tcggtgttga tcatgccgcc
    14581 cagggtggcg cggttgctgg tggaaagctc cggcgcaaag aacaggccgt gaggttttaa
    14641 ggcggcgttg agctgatctt tcaccacccc ggcctgcacg cgggcggtgc gggccactgg
    14701 gtctatggcg agaatctggt tcatatgttt ggacatatcc accactaccc caggggtgag
    14761 ggattgccca ttggtgccgg tgccgccgcc gcggggggcg atcaccaatg tttgaaaggt
    14821 ggtattggcg gccagctcga gtatgtacac caggtcctgc tggtctttgg ggtagagcac
    14881 cgcttggggc agcacctgat aaatggaatt atcggtggcc agcaccgtgc ggctggcgta
    14941 gtcgctgtga atatcgccct taaatcccag cgccgctaag gtgtgtagga atttttcggt
    15001 gagtgcatca aggccgggag tggtaagggc gggcagcatg gctaaggtcg cagctaaaag
    15061 tgggcattct actgggcttg gcaagttcag cactagtatt tagggccgcc tggcagccac
    15121 agcactgtat acgtcaatcc agtgcctgcg gctgaatcgt gttctccaac ccttgcggcc
    15181 aaaaaatttt ccagaatgcc tattaggtac tgcggaaggc ccccggcggc ggttcaatcc
    15241 gcggcgcggg tgcgctaagc ttcctgcccc gtcaattaag gccgcccagg agcaagcatg
    15301 cagtcatttc tcgttcagtt gcgccgccct ctgtgggccg tccttgtggc cgccgcgctc
    15361 ctgcttggat tcttaagcca cagccttgtt atccagttgc ataatttcag cattaaaagc
    15421 gccgctggcg agcctagccc cattaacgtg ccctttttaa aacagggcac tggcgctatg
    15481 gattatgttg ttaagggcga gcttacattg ggctggctgg caccctcctt atataacgtg
    15541 atacccgatg acctgttgct aaaactggtg ataaacggcc aagaggtaga cctttccgat
    15601 cagccctatg agcgcctgaa agatgtgaac cagggtgtga cgctgaattt agggcgcttt
    15661 ttgcacaccg gtagcaactc aatagaagtt cacttccgcg atttcagcgg cgacatgggc
    15721 ctgaccatca aaaccagccc gttggatgtg cgggtgttag gcctgtttat cgcctgggtg
    15781 ttggtggcga gtatcgcggc ggttgccctg ctgcagtgct tcggcctgag ccgagtgcgc
    15841 agcgtcctct actggctgat cgccgcaggc agtgtgatcc gcgtggccta tatctttacc
    15901 tacaatccgt tggatcacat cttcagcgat ccccagcgcc attgggagca gggcacggag
    15961 gtgttgcgcc gggacctgat ggccttaacc gaccccatcg gctatcagat atacgtaggg
    16021 gcgctggcca aactgagcgt taaattgcct gagctggtgg cgttttacac ctccctgctc
    16081 gccctcctgg gcgcctggtt ttggtaccgt ttcttgcggg agctacaggg cagtaaaacc
    16141 ctggccttag ccggttgggc gtttttaagt ctgctgccct cctggacggc catttacgcc
    16201 tattttatgc aggaaaccct ctttatcgtg ctgctgggcg ccgccctctg ggccacctgg
    16261 cgctgccgcc gcaaagcaga tgcagccagt ttcgccgcca tggtgttttt ctggacggcg
    16321 gcgggtttaa cccgtggcat tgccattcct ttagcagcgg tggcctgcac ctggctttgg
    16381 tttttgcagg ctggcaaggt gagaaaagcg ctttattcaa gcgcaatttt gctgtttatc
    16441 ctcggccccc tcaccgcccg cacctatcct ttggtgcatc atttcgcccc ccacggcatg
    16501 gggcagctca atgtcatcta tgcccaatcc ggcaaaaaag tcattgaagt gaa
//
LOCUS       DAOQGS010000011        16193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1647_length_16193_cov_5.047713, whole genome shotgun sequence.
ACCESSION   DAOQGS010000011 DAOQGS010000000
VERSION     DAOQGS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 16193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16193
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1647_length_16193_cov_5.047713"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKZ52_00890"
     CDS             <1..337
                     /locus_tag="PKZ52_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNG58348.1"
                     /translation="VAPNTPLNQALEEISAKGLGMTTVVDESGALLGIFTDGDLRRAV
                     SRGADLNQASIGQLMSANPRRVALETMAVEALNLMEQYRITSLVVVDHQQHPVGVLHM
                     HHLIKAGLV"
     gene            338..865
                     /locus_tag="PKZ52_00895"
     CDS             338..865
                     /locus_tag="PKZ52_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNG58349.1"
                     /translation="MNEQLQARARAIRLLVLDVDGVLTDGRLYFGSDGEALKTFNTLD
                     GHGLKMLAASGVQVAIITGRNSPMVLRRAEGLGISRIKLGREDKFAALQELLNDEPCP
                     LEAIACMGDDYPDLAIMSRVGLALAPPNACDEVRVRAHWISTRPGGEGAVREACDLIM
                     RAQGTYFAALAPYLA"
     gene            873..1469
                     /gene="lptC"
                     /locus_tag="PKZ52_00900"
     CDS             873..1469
                     /gene="lptC"
                     /locus_tag="PKZ52_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNG58350.1"
                     /translation="MRLRTLSIIIATAAGLLLAIWLGRSASQGLSLPQLAPLVSQPNS
                     YALLRSTQSKHYNQKGELAYSYKAASMEYFRADISQTSERDYSLVQAPELTFFGDGLP
                     WFIHAKEGKITDQGDTLQVWGDVRVWQVNAAGQQTLLTTQSLTIKPRQQEITTADPVA
                     ITSPTGSMGAEGLTVNLETHILKLHSKVKGSHDPVSTR"
     gene            1447..1983
                     /gene="lptA"
                     /locus_tag="PKZ52_00905"
     CDS             1447..1983
                     /gene="lptA"
                     /locus_tag="PKZ52_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNG58351.1"
                     /translation="MIQFLRVSFTCLLLYSAPLWALQGDRSEPISIESDTAERDETKG
                     TTTYAGAVIMQQGSMKINADKVIIYSNKEKVTHIIATGKPVHYEQKPSEKQALVIAQA
                     NTLEYQIQDESLHLIDSAFLEQEGTNLSGNRIDYDVKNSVVKAGGETKNRERVRMVIN
                     PKVLNTDDKTDTKTDVKP"
     gene            1998..2723
                     /gene="lptB"
                     /locus_tag="PKZ52_00910"
     CDS             1998..2723
                     /gene="lptB"
                     /locus_tag="PKZ52_00910"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNG58352.1"
                     /translation="MKKLQALNLAKSYKKRQVVIDVSIEVEAAQIVGLLGPNGAGKTT
                     CFYMIAGLVKADKGRVLINGQDISRAPMHGRARQGLGYLPQEASIFRKLSVEDNILAI
                     LETRRELSSAQRQQILEQLIAEFHIGHIRKSLGMALSGGERRRVEIARALATDPQFIL
                     LDEPFAGVDPISVNDIKQIIIHLRERGIGILITDHNVRETLDICEKAYIVGEGHIIAA
                     GTPADVLNNAKVRQVYLGEHFHL"
     gene            2795..3265
                     /locus_tag="PKZ52_00915"
     CDS             2795..3265
                     /locus_tag="PKZ52_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58353.1"
                     /translation="MRLFLLMFLLLAGCSSIKPEKKSDEKISKVLLGDWTWISTFNGC
                     PEYGDVAFRSNGTYTMTTEDCLMADDGFGIFQYGWFVANNYICFVYDERQLLEIAPSS
                     KELKAFLKSKKDEGFNKDYCGWEVVKYSSGSVVVRYAREREGGVEKFFTLKKSH"
     gene            3347..3922
                     /gene="smrA"
                     /locus_tag="PKZ52_00920"
     CDS             3347..3922
                     /gene="smrA"
                     /locus_tag="PKZ52_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HNG58354.1"
                     /translation="MALDEDFEQFFGRDVKPLNIKPRADLKRAAAADEKAQALRRERA
                     QQELKKALDPLAGEPVELLAPLDIMSFVRPGVQTGVFRSLRLGKYPLDARLDLHKLSA
                     DQARGEVYQFIKDCMANEVRTALITHGKGEGRTDSALLKSCVNYWLPQMPEVLAFHTA
                     QKQHGSYGATYVLLKKSERQKQLTREQFTRR"
     gene            3935..4330
                     /locus_tag="PKZ52_00925"
     CDS             3935..4330
                     /locus_tag="PKZ52_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNG58355.1"
                     /translation="MLYPLYAQVVLTFLVLFWAFFTRIQAVRQREINFGYFRLMQGAA
                     PERMQKATRQFANLFEMPVLFYAVCLLCVFKGFADTAQVALAWIYVVCRSAQALIHLS
                     YNHILHRVVVFGLGNAVLLALWLRVMLHV"
     gene            4350..4964
                     /locus_tag="PKZ52_00930"
     CDS             4350..4964
                     /locus_tag="PKZ52_00930"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HNG58356.1"
                     /translation="MRLILVVLALLLSACSTLSPEECKQADWYLIGMTDAQKGYGKVR
                     IDEHRKACAKVQVTPDLFTYQRGHEKGQKTYCTTANGFTQGSAGAQYQGACEGFLAED
                     FLRGYRDGQELYQARSRVDQLYRDIDSYHRDLDRMGREIAEKQSQMAYESNSDRRRDL
                     LTRIQQLQNDVQYTQNNLYNAERERDPAEANYRRVQQRMRSFGY"
     gene            5029..5682
                     /locus_tag="PKZ52_00935"
     CDS             5029..5682
                     /locus_tag="PKZ52_00935"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNG58357.1"
                     /translation="MELITFLVDFILHVDVHLVELFNQYGLWIYAILFCIIFCETGLV
                     ATPFLPGDSLLFAAGALMAASNLNVHSLALLLWLAAVSGDSTNYTVGRFLGARLFANP
                     NSKIFRTDYLLKTQGYFDRYGGRTLIMARFMPIVRTFAPFVAGMGKMPFARFIRFSVL
                     GGFLWVFSFIYAGFYFGGIPAVRKNFTLLILGIIFVSFLPIFIELWRARLRGRQVAE"
     gene            complement(5701..6606)
                     /locus_tag="PKZ52_00940"
     CDS             complement(5701..6606)
                     /locus_tag="PKZ52_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG58358.1"
                     /translation="MHDYTTNRPGISRRQLLKAAAMGACSSAVPVAPMVWAKATEELR
                     IMSRGDVNSNYALGAIKLALGKAGVPFKLNISEGDFAPMRQRKELIEGGLDILWTATS
                     IDMEEVALPIRVPLYKGLLGHRILIVHKDNKNLFNQVDTWDQMLAYRFGQGRDWSDTT
                     IMRANGMKVVTAVKYDNLFLMADGKRFDAFPRGVHEPWAEIEKRPSLELAVDEHVMMV
                     YRMPFYLFVSPNKKSFAEDLYQGMLMAVDDGSFDEYFYTNPTVKMVLEKANLKNRVIF
                     NLKNPALPPKTPVDDARLWVNISKL"
     gene            6932..8044
                     /locus_tag="PKZ52_00945"
     CDS             6932..8044
                     /locus_tag="PKZ52_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58359.1"
                     /translation="MAQALKLFFSRHKVAQLAAAIAAVYPAFNSCAFMDFCCDGLEHL
                     ELMPRAQRLSAGLRRYLPADYEQAIEIIMLSLGPPLGAAENNGLEPFFYLPHVFFVRD
                     YGLGHFAVSMAAQYELTQRFTAEFSLRPYFETHYAACMALLVRWAQDPSEHVRRLVSE
                     GTRPRLPWAPKLARVIQQPQLTAPLLELLKDDPSLYVRRSVANHLNDVGKDHPDYMLQ
                     VLEGWAQDAPVPRQWLIKHALRSAVKRGNSRAIGVLGFAGADGVAVASVALTPAKPQL
                     GQTLVLRAQLALAADAKDALDVLVDVKMDFPGINGRRQKVFKITSGRLAPGAALPLHK
                     SISLQPMTTRTYYPGLFIFTLLVNGQPLPLIEVDIV"
     gene            8325..8702
                     /locus_tag="PKZ52_00950"
     CDS             8325..8702
                     /locus_tag="PKZ52_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58360.1"
                     /translation="MRTMHPKVLSIVLSLPCACVLAPLLMNVPWLHYFMGYSLYSHYE
                     NVFLLTAISVYGLAQLVIFRLRHGYWRAVFHRVAFYVVSAEAVLVLGLVSLALLWVVP
                     MALLLFSDSGREAWQPGARQVNI"
     gene            8905..13125
                     /locus_tag="PKZ52_00955"
     CDS             8905..13125
                     /locus_tag="PKZ52_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG58361.1"
                     /translation="MDVLRVLLCLTVVLVSARGFACSDTKELRFAHWMEESNSTNESI
                     GAVNTIAQDVHGVIWAGGQNGLARFNGSHFSLFRYSINDPSSISNSYVSAITSDSTGA
                     LWVATRAGLNRFDPRTERFQRFDRLNGALPADNINSLAVAGDRLWIGTTQGLWLLNPG
                     ASQAEEIIPLRGQDIRALFPAEGGELWLGTLNGLGLWQPEQARFTWRGNRALDVRALA
                     QDRQGNLWVGSFNHGLWRVNPDSDEWRSFGKEEGLIGQLIWALEFNQRGELWVASELG
                     GVSRFDPVQERFFASTWHSSIPASLASNKAKALKTDALGDLWVGLFPNGIDHASRFAN
                     QFCNYQGADDDRGLSHRSVLSLLPSAQNPADLWVGTEHGLNYFDASTGRFSHFLPEPG
                     VAGRLQSSAILTLAQGERPEEIWVSAWGSGVYRYNSQSRIFRQIPVRPGNAQALQTPY
                     VWAILRAQDGTFYLGGESGGLYIYRGGDLFESLPPVQNDPNSISSGFVRSLLQTRNGR
                     IFVGTPAGLDEYLPEKKAFAHWLRDEQGLFRIGASISVIFESADGQLWLGTEGAGLIR
                     FDPSSHAYRLFTQADGLPSDFIAAINQDKDGVIWLSTLRGIARLKGERFEALDKNLGP
                     VSNNFNRDASLYMPNGQLWFGSAEGLTHFDPLTLMTEAASPGSVHLEALRLLNKPQVI
                     GSSLLPEALDYVPEIVLGSDSPMVSIDFYALNYRAPQLNRFAYKLVGVDADFIDIGNQ
                     RSATYTHLPAGRFTFQVKAADSLGQWHPQVKELRIEIKPAWWRSYWAFALYGLIVSLA
                     LLALWRTQQRRRDLVQAQALNEKLRQLDQLKDTFLANTSHELRTPLNGIIGLAEALLE
                     GSQGPLSEGVHKTLKIISSSGRRLSYLINDILDFSKLSKRDLQLNTRALSLRPLLESV
                     LELVRPLIGDKPLLLFNEVPASLPNVLADPNRLQQILLNLIGNAVKFTPAGSVRVRAD
                     VVNGRVEIHVRDTGIGIDPKDLDAIFLEFSQVDSSEVRAQGGTGLGLAIAKRLVHLHG
                     GQLQVKSKLGQGADFYFALNIATDSAHVDNTPPEKAGGTLDINAATLEKTPDNSNTLL
                     YPKDIIGRFTVLVVDDDAVNRMVLTSILKLHQHRVIEVNSGQQALELLAKDNNIDIVI
                     LDVMMPIMSGFETAMRIRVQYPVHLLPIIFLTAKNYSDDLVRGFVAGGNDFLTKPVSK
                     QELLTRVTSHLRLLQINRTLEENLKTRNFEAASTQVELQALDTIIASLNREMNPQILL
                     KTLLNQMLLLVQHADGASLWQLSGTRGWICSAALALDHKYLGEQCFSVDEALVNYLTS
                     LGQSHQPIHALRDFKNTPLAPLYNFFEQPEHTLIAVAVAENNLVGYIALSYTGEPPTI
                     DEYLVNAINRIKAHATSVLLKANMMQLN"
     gene            complement(13140..13739)
                     /locus_tag="PKZ52_00960"
     CDS             complement(13140..13739)
                     /locus_tag="PKZ52_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNG58362.1"
                     /translation="MRISKRFAWGAALAPNLAFAHVTHSEAAGISGGLLHTLTGLDHL
                     AALWCLGALAALFASSAKQHAPNSLAPQWLWLPGLFALGALAGLYCALQGLAINPEPI
                     IGFSVLFWGVLLVVRTRLHLAVQAIAAVLFAFFHGLAHGPVVSEIQAPAGFYAGLSIG
                     FGLCFGAGFFATTYLTAATARKLAGLVSLAGLSYFFAAG"
     gene            complement(13780..14397)
                     /gene="ureG"
                     /locus_tag="PKZ52_00965"
     CDS             complement(13780..14397)
                     /gene="ureG"
                     /locus_tag="PKZ52_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNG58363.1"
                     /translation="MTKPPLRLGIGGPVGSGKTALVKTLCGELKDTYHIAVITNDIYT
                     KEDAEFLLRHEALDADRILGVETGGCPHTAIREDASMNLSAVEELQHRFPNLDLIIIE
                     SGGDNLAATFSPELSDLTLYVIDTGAGDKIPRKGGPGITKSDLLIINKIDIAPMVGAD
                     LGVMDRDAKKMRGAKPFIFTNLKDGTGVADVVRFIEQQGMLTPSK"
     gene            complement(14442..15146)
                     /locus_tag="PKZ52_00970"
     CDS             complement(14442..15146)
                     /locus_tag="PKZ52_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNG58364.1"
                     /translation="MVGTIINPSDSTQTASLMHNLHLLHLCSPALPIGAYAYSQGLEY
                     AIDSGWLKTPDAIADWLLGLMENTLAYTDLPILRRLYLAFAACDCAGANEWNNYLRAC
                     RESRELLLEDEQLGGALARLLKGLGVQVLELTAPPTYASQFAQAGVFWGIPLNSLCQG
                     FLWAWLENQVAAATKLVPLGQSQGQQILVSLMPHVPAVCAAAAVRQDAELGRGLPGLA
                     MASSLHESQYSRLFRS"
     gene            complement(15127..15615)
                     /gene="ureE"
                     /locus_tag="PKZ52_00975"
     CDS             complement(15127..15615)
                     /gene="ureE"
                     /locus_tag="PKZ52_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNG58365.1"
                     /translation="MSESSLQFTALLEAHALADVGTAGNLLVVASFSERQKSRHKTQT
                     QCGKPVGWFLPRGLVLKEGDVLLCRSGETIAIQAAPEDCSQVESSDALKLMTAAYHLG
                     NRHVPLQIGPGLLRYQRDHVLDAMLEGLGLPVHHEHLPFQPESGAYHGKSVLQLDGGH
                     HH"
BASE COUNT         3716 a         4113 c         4425 g         3939 t
ORIGIN      
        1 agttgcaccc aatacacccc tcaaccaggc gcttgaggag atttccgcca agggcctagg
       61 tatgaccacc gtggtggatg agagcggcgc cctgttgggc atcttcaccg atggcgacct
      121 gcgccgggcg gtgagccgag gggcggattt aaatcaggcc agcatcggcc agttgatgag
      181 cgcaaacccg cgccgggtgg cccttgagac tatggcggtg gaggccctca acctgatgga
      241 gcaataccgc atcaccagcc tggtggtggt ggatcaccag cagcaccccg tcggcgtatt
      301 acacatgcat catttaataa aggcaggctt ggtataaatg aacgaacaac tacaagcgcg
      361 ggctcgggcc attcgtttat tggtgttgga cgtggacggt gtgctcaccg atggccgcct
      421 gtatttcggc agcgacggcg aagccctgaa aacctttaat accctggacg gccacggcct
      481 gaaaatgctc gccgccagcg gcgtacaggt ggctattatc accggccgca acagccccat
      541 ggtgttgcgc cgggccgaag gcttgggcat cagccgcatt aaactcggcc gcgaggataa
      601 atttgccgcc ctgcaggagc tgctcaacga tgagccctgc ccattggagg ccatcgcctg
      661 catgggggat gactacccgg atttagctat tatgagcaga gtgggcctgg ccctagcgcc
      721 ccccaatgcc tgcgatgagg tgcgcgtgcg cgcccattgg attagcactc ggcccggtgg
      781 tgaaggcgcc gtgcgggaag cttgcgattt aatcatgcgc gcccagggca cctattttgc
      841 cgccctggca ccctatttag cctaagttgt ttatgcgcct gcgcacactc agcatcatca
      901 ttgccacggc ggccgggtta ctgctggcca tttggctggg ccgcagtgca agccaaggcc
      961 tgtcgctgcc ccagcttgcc ccactggtca gccagcccaa cagctacgca ctgctgcgct
     1021 ccacccagtc aaaacactac aaccaaaaag gcgagctggc ctacagctac aaagccgcca
     1081 gcatggaata tttccgcgcc gacatcagcc aaaccagcga gcgagattac tccttagtac
     1141 aagcccctga gctgactttt tttggcgacg gcctgccgtg gtttattcat gcgaaagagg
     1201 gcaaaataac cgaccagggc gatacattgc aggtgtgggg tgacgtgcgg gtatggcagg
     1261 tgaatgcggc cgggcagcaa acccttctca ccacccaaag cctcaccatc aaaccccgcc
     1321 aacaggaaat caccaccgcc gatcccgtcg ccatcaccag ccccaccggc agcatgggcg
     1381 ccgaagggct gacggtaaat cttgaaacac atattctcaa attacattcc aaggtgaagg
     1441 gcagccatga tccagtttct acgcgttagt tttacctgcc tgttgttata cagcgcgccg
     1501 ctgtgggcac tgcaggggga ccgcagcgag cccattagta ttgaatcgga caccgccgag
     1561 cgcgatgaaa ccaaaggcac caccacctac gcaggcgccg tcatcatgca gcagggcagc
     1621 atgaaaatta atgccgataa agtgattatt tacagcaata aagaaaaggt cacccatatt
     1681 attgccaccg gcaaacccgt ccattacgaa caaaaaccca gcgaaaaaca agccctagtc
     1741 atcgcccagg ccaatacctt ggaatatcaa attcaggatg aatccctgca tttaattgac
     1801 tcggcatttt tagagcagga aggcaccaat ttaagcggca accgcatcga ctacgatgta
     1861 aaaaattcgg tggtaaaagc cggtggagag acgaaaaatc gcgagcgggt gcgcatggta
     1921 atcaacccca aggtgctcaa caccgatgat aaaaccgaca ccaaaactga tgtcaaacca
     1981 tgagtagcag cgcggcggtg aaaaaattac aggcgttaaa tctggccaaa agctacaaaa
     2041 aacgccaggt ggttattgat gtctccattg aggtggaagc ggcgcaaatt gtcggcttac
     2101 tcggccctaa tggggcgggt aaaaccacct gtttttatat gattgcaggc ctggttaaag
     2161 ccgataaggg ccgggtgctc attaacggcc aggatattag ccgcgcccct atgcacgggc
     2221 gggcgcgtca ggggctgggt tatctgccgc aggaggcgtc tatttttcgt aaattatcgg
     2281 tggaggataa tatcctggcg attttagaaa cccgccgaga attatccagc gcccaacggc
     2341 aacaaatttt agagcagtta attgccgaat ttcatatcgg ccatattcgt aagagtttag
     2401 gtatggccct atccggcggt gagcgccgcc gggtggaaat tgcccgcgcg ctggccactg
     2461 atccgcaatt tattttattg gatgaaccct ttgccggtgt ggaccctatt tcggtaaacg
     2521 acatcaaaca aatcattatt cacctgcgcg agcgcggcat cggcatttta attaccgacc
     2581 acaatgtgcg cgaaaccctg gatatttgtg aaaaagccta catagtgggc gaggggcata
     2641 ttattgctgc gggtacgcct gcagatgtgc ttaataatgc caaggtgcgc caagtgtatt
     2701 tgggtgagca ttttcatttg taaaccatcc gctcggctga gcgtatattt tgcgatccat
     2761 tcgcattgtg agtttttttc gtaggggaaa ttccgtgaga ctatttcttc ttatgttttt
     2821 gcttttggct gggtgctctt cgataaagcc agaaaaaaag tctgatgaaa aaatttcaaa
     2881 agttcttttg ggtgattgga cttggatttc tacgtttaac ggttgtccag agtatggtga
     2941 tgttgcattc aggtccaacg gcacttacac aatgacgact gaagattgtt tgatggcaga
     3001 tgatggcttt ggaatctttc aatatggttg gtttgttgca aacaactata tttgctttgt
     3061 ttatgatgaa cgacaattgc ttgaaatagc gccatcgagc aaagagttaa aggctttttt
     3121 aaagagtaaa aaggatgagg gttttaacaa ggattattgt ggctgggagg ttgtaaaata
     3181 ttcttccggg tcggtggtgg tcaggtatgc ccgggagcgc gagggaggcg ttgaaaaatt
     3241 ttttactctt aagaaatcac actagcaatc aatatattgc attggcctac aaagatgggg
     3301 cacctagact gccgtcaaac cgcctgtaaa ttttgaggac tccaacatgg ccttggatga
     3361 agattttgag cagttttttg gacgtgatgt taagccgctt aacatcaaac cccgcgcgga
     3421 tttaaaacgc gcggcggcag cggatgaaaa agcccaagct ctgcggaggg agcgtgcgca
     3481 acaggaatta aaaaaagctc tcgacccatt ggcgggggag cctgtggagc tgttagcgcc
     3541 cctggatatt atgagttttg tgcgaccagg cgtgcagacc ggggtatttc gcagcctgcg
     3601 cttgggtaaa tacccattgg atgcgcggtt ggatttacac aaactcagcg ccgatcaagc
     3661 ccgcggcgag gtgtatcaat ttattaagga ttgtatggcc aatgaggtgc gcacggcctt
     3721 aattacccac ggcaaaggcg aggggcgcac agattctgcc ttattaaaat cctgcgttaa
     3781 ttattggctg ccgcagatgc ctgaagtatt ggcctttcac actgcgcaaa aacaacacgg
     3841 ctcctatggc gccacctatg ttttattaaa aaaatccgag cggcaaaaac agctgacacg
     3901 ggaacagttt actcgtcgat aaagtaggcc aggcatgtta tatccgctct atgcacaggt
     3961 tgtattaacg tttttagttt tgttctgggc tttttttacc cgcatacagg cggtgcgcca
     4021 gcgggaaatt aattttgggt atttccgttt aatgcaggga gctgcgccgg agcgcatgca
     4081 aaaagccacc cgccaattcg ccaatttatt tgaaatgccg gtgttgtttt atgccgtctg
     4141 tctgttgtgt gtatttaaag gttttgccga tacagcgcag gtggcgctgg cctggattta
     4201 tgtggtctgt cgcagcgcac aggcgctgat tcacctaagc tataaccaca tactgcacag
     4261 agtggtggtg tttgggctgg gtaatgcggt gttattagcc ctttggctgc gcgttatgct
     4321 acacgtttaa tttttctaag gcccagtcca tgcgattaat cctcgtcgtt ttagcgctac
     4381 ttctctccgc ctgctccacc ctcagccccg aagaatgtaa gcaggcggat tggtatttaa
     4441 tcggcatgac ggatgcgcaa aaaggttatg gcaaggtgcg catcgatgaa caccgcaaag
     4501 cctgcgctaa ggtgcaggta acccctgatc tatttaccta ccagcgcggc catgaaaaag
     4561 gccaaaaaac ctactgcact acggccaatg gttttaccca gggttcggcg ggcgcccagt
     4621 atcaaggtgc ctgtgagggt tttttagcgg aggatttttt gcgcggctac cgagacggcc
     4681 aggagttgta ccaagcccgc agccgagtgg atcaactcta tcgggatata gattcttatc
     4741 atcgcgacct tgaccgcatg gggcgagaaa ttgctgaaaa acaaagccag atggcctacg
     4801 aaagcaatag cgaccgccgc cgggatttat taacgcgcat ccaacaatta caaaatgatg
     4861 tgcaatacac ccaaaataat ttatacaacg ccgagcgtga gcgggacccc gccgaagcca
     4921 attaccgcag agtgcagcag cgtatgcgca gtttcggtta ttaagtggcg agctgctacc
     4981 tatggccgtg ggcaagaatt tctaatttaa tcatctgaga gtgtgactgt ggagctaata
     5041 acgtttttag tggattttat tctgcatgtt gatgtgcatt tggtggagct gtttaatcaa
     5101 tacggcctgt ggatttacgc aattttattt tgcattattt tttgcgaaac cggtttggta
     5161 gccacgccgt ttttgcctgg cgattcgctt ttgtttgccg ccggtgcact catggcggcg
     5221 agcaatttga atgttcacag cctggctttg ttgttgtggc tggcggctgt gtctggcgat
     5281 agcactaact atacagtggg gcggttttta ggtgcccgtt tatttgccaa ccccaactcg
     5341 aaaatattca gaaccgatta tttgctcaaa acccaagggt attttgatcg ctacggcggc
     5401 cgcactttga tcatggcgcg ctttatgccg attgtgcgta cctttgcacc atttgtggcg
     5461 ggcatgggta aaatgccttt tgcgcgcttt attcgcttta gtgtgttggg cggatttttg
     5521 tgggtgtttt cgtttattta tgccgggttt tattttggtg gtatcccagc ggtgcgcaaa
     5581 aactttaccc tgttgatttt gggtattatt tttgtgtctt ttttacctat ttttatcgag
     5641 ttgtggcgcg cccgccttag gggcagacag gtggccgagt agcggtgcgt tgcggcggtt
     5701 ttagagttta ctgatattca cccacaggcg ggcgtcatcg accggtgttt tcggtggcag
     5761 ggcggggttt ttgaggttga atatcacgcg gttttttaag ttggcttttt ccagcaccat
     5821 ttttactgtg ggattggtgt aaaaatattc atcaaagctg ccgtcgtcca cggccattaa
     5881 catgccctgg tagaggtctt cggcaaaact ttttttgttg ggagacacaa acaaataaaa
     5941 cggcatgcgg tataccatca tgacgtgttc atccaccgca agctcaaggc ttgggcgttt
     6001 ttcgatttcc gcccagggtt catgcacccc gcgcggaaag gcgtcaaagc gtttgccatc
     6061 ggccattaaa aataaattgt cgtatttgac ggcggtaacc accttcatac cgttggcgcg
     6121 catgatggtg gtatcggacc aatcccgccc ctgtccaaag cggtaggcta acatctgatc
     6181 ccaagtatca acctggttaa ataaattttt attgtcttta tgcacaatta aaatccggtg
     6241 ccccaataaa cccttgtaca aaggcactcg aatcggcagc gccacctctt ccatatcgat
     6301 ggaggtcgcc gtccagagaa tatccaggcc gccctctatt aattccttgc gctggcgcat
     6361 gggggcaaaa tccccttcgc tgatattgag tttaaagggc acgcccgctt tgcctaaggc
     6421 taatttgata gcgcctaagg cgtaattact gtttacatcg ccccgcgaca taatgcgcaa
     6481 ctcttcggtt gctttggccc acaccatcgg cgctacgggc acagcgctgc tgcaggcgcc
     6541 catggcggcg gctttgagca attggcggcg gctgataccg ggtctgttcg ttgtgtaatc
     6601 gtgcatggat gtgtagggtc ggcaagggtt ggactaaaag cacgggggtt ataaattaac
     6661 gttagcccga cgagggccga tattcaaata atggcagggc ttatgagcaa atattcgcga
     6721 aaaaaggctt aaacagcgcg aaagctccca gtgtgagctg ataaataatc tcaaagcctg
     6781 tgttgtgcga tggggaaaat acccagtttg atcggcgcat gatttgctgc gcgagagtct
     6841 atgaaagtag ctttgccgcc cagtcactaa ggtgttttta cgctgttttt gtatgcgctt
     6901 gcgcgcggga tttttaataa aaaaggttgt tatggctcag gcgttaaaat tatttttttc
     6961 ccggcataaa gttgcccaat tggcggcggc tattgccgct gtatacccgg cgtttaatag
     7021 ctgtgcattt atggattttt gttgtgatgg cttggagcac ttagagctga tgcctagggc
     7081 gcagcgttta agtgcagggc tgcgccgtta tttgccagcg gactatgaac aggctattga
     7141 aattatcatg ctctccttag ggccgccttt aggcgctgcg gaaaataacg gcttagagcc
     7201 gtttttttat ctgccccatg tgttttttgt gcgggattat ggcctggggc attttgcggt
     7261 ttccatggcg gcacaatatg aactcaccca gcgctttacg gcggaatttt ccctgcggcc
     7321 ttattttgaa acccactatg cagcctgtat ggcgctgctt gtgcgctggg cccaggaccc
     7381 aagcgagcat gtgcgccgtt tggtgagcga aggcacccgg ccgcgcctgc cctgggcgcc
     7441 caagctggca agggtcattc aacagccgca gcttacggcg ccgctactgg aattactgaa
     7501 ggatgatcca tcgttatacg tgcgccgctc ggtggccaat catttaaatg atgtgggcaa
     7561 agaccatccg gattatatgc tgcaggtgtt ggagggttgg gcgcaggatg cgccagtgcc
     7621 gcgccagtgg ttgatcaaac atgccctgcg ctcggcggta aaacgcggca atagccgcgc
     7681 tataggggtg ctgggttttg caggtgctga tggcgttgcc gtcgcgtcgg ttgcgctgac
     7741 accggccaaa ccacaactgg gccaaacgct tgtgctgcgc gcgcaacttg ccttggcggc
     7801 agatgcgaag gatgccttgg acgttttagt ggatgtcaaa atggatttcc ccggtattaa
     7861 tgggcggcgg cagaaggtat ttaaaatcac cagcgggagg ctcgcgcctg gcgctgcctt
     7921 gccactgcac aaatccatca gcctgcaacc catgaccacc cgcacctatt accccggcct
     7981 ttttatattc accctgctgg tgaatggcca gccgctgccg ttgattgagg tggatattgt
     8041 ttagtggccc gctagtagct cggcataaaa agccgccgtg ttaatcgtta ccaggcggtt
     8101 aagcctgcgg ccatagatta atttcgtatg gaaataaccg cttgagtgtt caagggctat
     8161 taaaaccctg cttatgcgct gcaaatcggc ttcgaacggt gcgcttaaaa gcaatattta
     8221 taaaaaccct caattcagat taaaacgctc caaggcgcca ccggttaatc ccaacaactt
     8281 atctacaatt ggaatattgc agtgagttaa aggaaagcca aatcatgcgc accatgcatc
     8341 caaaagtatt gagtattgtg ttgagcctgc cttgcgcatg tgtgcttgcg ccgcttttga
     8401 tgaacgtgcc ctggttgcat tatttcatgg gctattcgct ctactcccat tatgaaaatg
     8461 tttttttact cacggccatc agtgtgtacg gcttggcgca gctcgtgatt tttcgcctgc
     8521 ggcacggcta ctggcgggcg gtttttcacc gcgttgcatt ttatgtggtc tcggctgagg
     8581 cggtattagt gttgggtttg gtgagcctgg cgctgttgtg ggtggtgcct atggcgctgt
     8641 tactgttcag cgatagcggc cgggaggcct ggcagccggg tgcgcgccag gtgaatattt
     8701 agttttttac gaacaacgcc atgttgtgag tccttggccg gtctgccggc cttttttatt
     8761 tgttcgccgc taagctatta gcggctttta atctgggtgc ctggccatcc ggtggagtat
     8821 ttattcactt ttcgtgttaa tcgccgggtt aattgaaggc tgctgactat actggcataa
     8881 attaaccctg attttgcgtt gccgatggat gtactccgcg ttttactctg tttaactgtt
     8941 gtgctggtta gcgcgcgggg tttcgcctgt agcgacacaa aagagttgcg ctttgcccat
     9001 tggatggagg agtccaactc gaccaatgaa tccatcggcg ccgtcaacac catcgctcag
     9061 gatgtgcacg gggtgatctg ggccgggggg caaaatggcc tggcgcgttt taacggcagc
     9121 cacttcagct tgtttcgtta ctctattaac gatccttctt ctattagtaa ttcctacgtt
     9181 tccgccatca ccagcgactc aaccggggcg ctttgggtgg ccacccgcgc aggtctcaac
     9241 cgctttgacc cccgcactga acgcttccag cgctttgatc gcttaaatgg tgcgctgcct
     9301 gccgataaca tcaacagcct ggcggtggcg ggggatcgcc tgtggatagg cactacccag
     9361 gggctctggc tgttgaaccc cggcgccagc caagcagagg agataatccc gttgcgcggc
     9421 caggacatcc gcgccctctt tcccgctgag gggggcgagc tgtggctggg caccttaaat
     9481 ggcttggggc tgtggcagcc tgagcaggcg cggtttacct ggcgcggcaa ccgcgccctg
     9541 gacgtgcgcg ccctggcgca ggatagacag ggcaatctgt gggtgggcag ttttaatcac
     9601 ggcctgtggc gcgttaaccc cgatagcgat gaatggcgca gcttcggcaa agaggagggc
     9661 ttaattggcc agctgatctg ggcgctggag ttcaaccaac ggggtgaact ttgggttgcc
     9721 tcggagctgg gtggcgtgag ccgtttcgac ccggtgcagg agcgtttttt tgccagtact
     9781 tggcacagca gtattcccgc aagcctcgcc tcaaataagg ccaaagccct taaaaccgat
     9841 gctttggggg atctatgggt ggggttattt cccaatggca ttgaccacgc cagccgcttt
     9901 gccaatcaat tttgcaacta tcagggcgcc gatgacgacc gcgggctgag ccatcgcagt
     9961 gtgttaagcc tgttgcccag cgcgcaaaac ccggctgatc tgtgggtggg caccgaacac
    10021 ggcctgaatt attttgatgc cagcaccggg cgttttagcc attttctgcc cgaacctggc
    10081 gtggcggggc gtttacagtc cagcgccatc ctcaccctcg cgcaggggga gcgcccggag
    10141 gaaatttggg tgagcgcctg gggcagcggc gtgtaccggt acaacagcca aagccgtatc
    10201 ttccgccaga ttccagtgcg ccccggtaat gctcaggccc tgcaaacccc ctatgtttgg
    10261 gccatattgc gggcccaaga cggcaccttt tatttagggg gcgaaagcgg cggtctgtat
    10321 atttatcgcg gcggtgattt attcgagtcc ctaccgccgg tgcagaatga ccccaattcc
    10381 atcagcagcg gctttgtgcg cagcctgttg caaacccgca acgggcgcat ttttgtcggc
    10441 acccccgcag ggctggatga atatctgccg gagaaaaaag cctttgccca ctggctgcgg
    10501 gatgagcagg ggctctttcg catcggtgcg tcgatcagcg tgatatttga aagcgccgac
    10561 ggccagcttt ggcttggcac cgagggggcg gggttaattc gctttgaccc gagcagccac
    10621 gcctataggc tatttaccca agccgatggc ctgccctcgg attttattgc ggcgattaat
    10681 caagacaagg atggggtgat ttggctgagc accctgcggg gtatagcccg ccttaagggg
    10741 gagcggtttg aggcgctgga taaaaacctc ggcccggtga gcaataattt taatcgcgat
    10801 gccagcctgt atatgcccaa cggccagctg tggtttggca gcgcggaggg gctcacccac
    10861 tttgacccgc tcaccctgat gaccgaagcg gcatcccccg gcagcgtgca ccttgaggct
    10921 ctgcgtttac ttaataaacc tcaagtcata ggttctagcc tgttgcccga ggccctggat
    10981 tatgtgccgg aaattgtcct cggctcggac agccccatgg tgtccatcga tttttacgcg
    11041 ctgaactacc gcgcgccgca gttaaatcgt tttgcttaca agcttgtggg ggtggatgcg
    11101 gattttatcg atataggtaa tcaacgctcc gccacttaca cccacctgcc cgctggccgg
    11161 tttacctttc aagtcaaagc ggcggatagc ctggggcagt ggcacccgca ggtaaaagag
    11221 ctgcgcattg aaattaaacc cgcctggtgg cgctcctatt gggcgtttgc gctttatggg
    11281 ttgatagtgt ccctggcgct gttggccctg tggcgcacgc agcagcggcg gcgggattta
    11341 gtgcaggcgc aggcgttaaa tgaaaaactg cgccaattag accaattaaa agataccttt
    11401 ttagctaaca ccagccacga attacgcacc cccttaaacg gcattattgg ccttgccgaa
    11461 gccctgttgg agggctccca ggggccctta agcgaggggg tgcataaaac cttaaaaatt
    11521 atttccagct ccgggcgcag gctgtcttat ctcatcaacg atattttaga tttctccaaa
    11581 ttatccaaaa gagatttaca gcttaacacc cgcgccctaa gcctccggcc gctattggaa
    11641 agtgttttgg aactagtgcg gccgctcatt ggcgataaac cgctgctgtt atttaatgag
    11701 gtgccagcca gcctgcccaa tgtgttggcc gaccccaacc gcctgcagca gatactgtta
    11761 aatttaattg gcaacgcggt gaaatttacc cctgcaggca gtgtgcgggt gcgtgccgac
    11821 gtggtaaacg ggcgggttga aatccatgtg cgcgataccg gcataggcat agatccaaaa
    11881 gacctggatg cgatattttt ggaattttct caggtggata gcagcgaggt gcgcgcccag
    11941 ggcggcacag gtttgggcct cgccatcgcc aagcgtttgg tgcatttaca cggcggccaa
    12001 ttgcaggtga aatccaaact cggccagggc gcggattttt attttgcctt aaatattgcc
    12061 accgatagcg ctcatgttga taacactccg ccggaaaagg ctggtggcac ccttgatatt
    12121 aatgctgcca ccttggaaaa aacccccgac aacagcaaca cacttttata ccccaaggat
    12181 attatcggcc gcttcaccgt gttagtggtt gatgatgacg ccgtcaaccg gatggtgctt
    12241 accagtattt taaaattgca ccaacaccgc gtaattgagg tcaatagtgg ccaacaggcg
    12301 ttggaattat tagccaaaga caacaacatc gatattgtga ttttggatgt aatgatgccg
    12361 attatgagcg ggtttgaaac cgccatgcgc atccgcgtgc aatatcctgt gcacctattg
    12421 ccgattattt ttctcaccgc caaaaattac agcgatgatt tggtgcgcgg ctttgtggcg
    12481 ggcggcaatg attttttaac caagcctgtt tccaaacagg agttattaac gcgggtcacc
    12541 agccatttgc gcctgttaca aattaaccgc accctggaag aaaatctcaa aacccgcaac
    12601 tttgaagctg cttccaccca ggtggagctg caggcgctcg acacgatcat cgccagttta
    12661 aaccgggaga tgaacccaca aattctatta aaaacattgt tgaatcaaat gttgttgctg
    12721 gtgcaacacg ccgatggggc cagcctctgg caattaagcg gcaccagagg ttggatatgc
    12781 tcggctgcct tggcgctgga tcataaatat ttgggggagc agtgttttag tgtggatgaa
    12841 gccctggtga attatttaac ctccctaggc caatcccacc agccgattca cgccctgcgg
    12901 gattttaaaa atactccgct tgcgccctta tataatttct ttgagcaacc tgagcacacg
    12961 ttaattgcag tggcggtggc ggagaataat ttggtggggt acatcgcact gagttacaca
    13021 ggcgagccgc ccaccattga tgagtatttg gtgaatgcca ttaaccgcat caaggcccac
    13081 gccaccagcg tgttactaaa agccaatatg atgcaattaa attaagcgca ccaactggcc
    13141 taacccgccg cgaaaaaata actcaagccg gcaaggctca ccaatcccgc caatttgcgc
    13201 gcagtggctg cagttaaata agtggtggcg aaaaaacctg cgccaaaaca caacccaaag
    13261 ccaatgctaa ggcctgcata aaacccagct ggcgcctgta tttcactcac cactggcccg
    13321 tgggcaaggc cgtgaaaaaa cgcaaacaac actgcggcaa tagcctgcac tgcaaggtgc
    13381 aaacgggtgc gcacaacaag taacacaccc caaaataata cggaaaatcc aatgatgggt
    13441 tcggggttaa tcgccagccc ctgcagggcg caatacagtc ccgccagggc acccagggcg
    13501 aataaacccg gtaaccagag ccattgtggc gctaggctgt tgggtgcatg ctgttttgct
    13561 gaggatgcaa atagggctgc caaggctccc aaacaccaca gggccgccaa gtgatccagg
    13621 cccgtaaggg tatgcagcaa accgccgctt atacctgcgg cctcagaatg agtgacatgg
    13681 gcaaaagcca ggttaggggc gagggcagcg ccccaggcga agcgttttga tatgcgcata
    13741 aataatctcc tcaaaagggt tattggttaa atacggcttt tattttgacg gcgttaacat
    13801 gccctgttgt tcgataaaac gcaccacatc ggcaacgccg gtaccgtctt ttaaattggt
    13861 aaaaataaaa ggtttggcgc cgcgcatttt tttcgcatcg cgatccatca cccctaaatc
    13921 ggcgcccacc ataggcgcaa tatcaatttt gttgataatt aataaatccg atttggtaat
    13981 gccggggccg cctttgcggg ggattttatc gcctgcgccc gtatcaataa cgtacagagt
    14041 taagtccgac agttccgggc taaacgtagc cgctaaattg tcgccgccgc tttcaataat
    14101 aatcaggtct aaattcggga agcggtgttg taattcttcc accgccgata aattcatcga
    14161 agcgtcttcg cgaatcgcag tgtgggggca gccgccggtt tccactccta aaatgcgatc
    14221 cgcatctaaa gcctcatggc gcaataaaaa ttcggcgtct tctttggtgt aaatatcgtt
    14281 ggtaatgacc gcaatatggt aggtgtcttt taactcgccg cagagggttt tcactaatgc
    14341 agtcttgccg gagcccacag ggccgccgat gcctaaacgt agagggggtt tggtcacggt
    14401 gaaggtctca tcaaagtaaa tggtttaaaa ataaaatgtt ttcaggagcg aaacagccgc
    14461 gagtactggc tctcgtgcaa acttgacgcc atggcaaggc cgggcaaacc tcgccccagc
    14521 tcagcatctt gccgcaccgc cgccgcagcg caaacagcgg gcacatgggg catcaggctc
    14581 accagtattt gctggccctg gctttggccc aagggcacga gtttggtcgc ggctgccacc
    14641 tggttttcaa gccaggccca caaaaacccc tggcagaggc tatttaaggg aatgccccaa
    14701 aacacccccg cctgggcaaa ttggctggcg taagtgggcg gcgcagttaa ttccaacacc
    14761 tgtacaccta agccttttaa taaacgggcc agggcgccgc ctagttgttc atcctccagc
    14821 aataactccc ggctctccct gcaggcccgc aagtagttat tccactcatt ggcgcctgca
    14881 caatcacaag cggcaaaggc tagatacaac ctgcgcaaga taggcaggtc ggtgtaggcc
    14941 agggtatttt ccatcaaacc caatagccag tcggcgatgg cgtcaggggt tttgagccag
    15001 ccgctgtcga tggcgtattc caagccctgg gaataggcat aagcgccgat gggcagggcg
    15061 gggctacaaa ggtgcagcag atgcaggttg tgcatcaaac tggccgtttg ggttgaatca
    15121 gaggggttaa tgatggtgcc caccatcgag ttgcagaaca gatttgccgt ggtaggcgcc
    15181 ggattccggc tgaaacggca agtgttcatg gtgcacgggc agccctaaac cctccagcat
    15241 ggcatcgagc acatgatccc gctggtagcg caacaggccc gggccaattt gcaggggcac
    15301 atggcgattg cccaaatggt aggcggcggt catcagctta agggcgtcgc tgctttccac
    15361 ctggctgcag tcttccggcg cggcctgaat ggcaatggtc tcgccgctgc ggcataacag
    15421 cacatcgcct tcttttaaca ccagccctcg cggtaaaaac caacccaccg gtttgccgca
    15481 ttgggtttgg gttttatggc ggcttttctg ccgctcgcta aagctggcaa caacgagcag
    15541 attgcccgct gtgccgacat cggccagggc atgggcctcc aaaagggccg taaactgtaa
    15601 gctggattcg ctcatggggg tgtcctaagg gcggttaaaa cgggcgaagc aaatagctca
    15661 ccaaaggccg cgtatctaaa cctatttcgc cattcatatc cagctgtggg ggcctgccgt
    15721 taagttaagg atttttggcg cctgcacgcc ctaaaattcc catcccgtag aggggtgcag
    15781 gcttgcctgc cggggggggg ctgcggagaa ctctgtttta tttcaggtgg cttttatttc
    15841 tggtggcttt tatttcaagt gacatttgta tcaggtgaaa aggaaatctt taattgcagc
    15901 gggctgcgga caaaaaacag cagagtgatc taccacccca ccccttcggg gcaccccgcc
    15961 acaggagggg atttttggcc ctgcccaggg tgaaaaaagg ccgccaactc ccaacggaat
    16021 gatatggcgc gctggaatga gcgggctgtg ttggtttctg aaggctttgt ggagaggggt
    16081 tttccctagc agcctgttga aatactgcct tgcgagcgtg ggcggcgtta aattttgaca
    16141 aaaaattctc atttactgcg tgtaaactcg gcaattgctc ctgcattgct cta
//
LOCUS       DAOQGS010000012        16015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1674_length_16015_cov_5.142105, whole genome shotgun sequence.
ACCESSION   DAOQGS010000012 DAOQGS010000000
VERSION     DAOQGS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 16015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16015
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_16015_cov_5.142105"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKZ52_00980"
     CDS             <1..550
                     /locus_tag="PKZ52_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG58366.1"
                     /translation="LSLSLQPGEALLIQGPSGVGKTTLLRACAGLWPYAQGQLTLPEG
                     SSLFLPQKPYLPLGTLKEALFYPNSPSEQALAPVLEQVQLGHLVARLDDEDDWGRVLS
                     LGEQQRLAFARALLIRPAVVFLDEASSAMDEGLEYALYQLLRQALPQSIVVSVGHRSS
                     LLQFHPQRLHLSGGGAWQLETA"
     gene            complement(877..1593)
                     /locus_tag="PKZ52_00985"
     CDS             complement(877..1593)
                     /locus_tag="PKZ52_00985"
                     /inference="COORDINATES: protein motif:HMM:NF006118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG58367.1"
                     /translation="MNLHNKTILITGANRGIGKALVNAALSFGAKKVYASARTIANLP
                     SFNDARVVPLQLDITDAASIQAAAQQATDVNVLVNNAGVLAFASVLSGDFADLHQDMN
                     VNYFGTLAVTRAFLPLIERNGDGAIASISSIIGLASMAGVGGYSASKAALASAIQAMR
                     AELTPKNIAVFGIFPGPIETDMAREFDLPKTSAAQTAQNIFTQMEQGLEDIFPDAMSA
                     ELGELWKTNPKGLEKQFSGH"
     gene            1754..2677
                     /locus_tag="PKZ52_00990"
     CDS             1754..2677
                     /locus_tag="PKZ52_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58368.1"
                     /translation="MNTQALQLFVAVARSGSFAAVARQRNQDPSLVSRSIAQLEAELG
                     VRLFQRSTRKMLLTEAGARLLEKVQALLEELSGALEEVASSAHEPSGTLRMTASVTFG
                     HHCVLPLLKEFRTLYPKIKIDLVLSDDPLDLIASHIDLAIRLGPSISGNLIVTKLLDC
                     HYRVVASPDYIASSAPIVQPKDLTAHACLLFPERIFAPDWQFKRPGEEPVVVRVSGDL
                     ILSTGDALRQMALRGMGPALLPHWAILDALEDGRLVDLFPTYQAAAASFNSAAWLVYP
                     SRDYLPKKVRVMIDYLKTHLPSRYAINSVPA"
     gene            complement(2785..3606)
                     /locus_tag="PKZ52_00995"
     CDS             complement(2785..3606)
                     /locus_tag="PKZ52_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58369.1"
                     /translation="MSALLSHPVFTDPQLLTGLLDACVLDGSVSHQTELQAIIEALHQ
                     TPEDALTPPAQAVLTALLAHPAWGGRGLVYNHCAKRLGQAVRPLADLKETWFHQLQQH
                     FVGGSGHFLAGSRVLFGQSQLVQACSRSMKKTILGLRQAGRPFAALTTEAKEFLASNE
                     AMALEKANGSQRGFIFADGSLVLEHKDDRAPRIFDRFIVSASTSAQGGQLMTRWKLFG
                     QYVFGLPEHDTAHAELLLHEQVRLRLPLSLVAKAQNLGLAKGFYYGADWMETWSG"
     gene            3739..4731
                     /locus_tag="PKZ52_01000"
     CDS             3739..4731
                     /locus_tag="PKZ52_01000"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HNG58370.1"
                     /translation="MLQLLNHTPFAAQIGLFPNEEGVDTLYVVAKAHFNMANPLSLCD
                     EQTPPRMEDVYYGEPGLSSLKYPSDFHPGKAATDVFMLGHACSPDKQPVYYLDTSLSL
                     GNLHKTIRVWGARTWQGGALSQPQPFTHLPIIYENAYGGQQVQDGQVVGGEERNPIGK
                     GFLGRQERSTFDGTAAPQLDDPFNPLRQLGDTPEPASYGPRAPGWLPRRAFAGTYDEA
                     WQQNRAPFLPEDYSPRFMNSAHPDLIADGFLSGGEAVKILGMHPLGELQFQIPRLSLH
                     TEITMGAQTHSPNFVLESILLEPNQLRFSLTYKARLICDKQALTIRQVALHLQR"
     gene            4757..5137
                     /locus_tag="PKZ52_01005"
     CDS             4757..5137
                     /locus_tag="PKZ52_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HNG58371.1"
                     /translation="MPQTTFANMRGIVHKGSNGMSLVFPDVCKTPTPAGPVPIPYPNL
                     GKSSDTMKGPTSVKTDKQMPMVKGAVYIMSTGDEAGSAMGVISNKIKGECEFMMYSFD
                     VKFEGKNVCRMGDPLFHNNKNIMG"
     gene            5140..6288
                     /locus_tag="PKZ52_01010"
     CDS             5140..6288
                     /locus_tag="PKZ52_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58372.1"
                     /translation="MNAPVNAFAHIIQQMADNASFLWVLRGRQRREPQVHPEHIAELD
                     ARIQANLDGLVLYGEDAWAIAQANGEFPQGGEAFCLGYLAFSSEDVSKIQYATEFALQ
                     NEDTFKGLVSALAWLPGLKVHPWITQFFHSKNLDHKRLALAACRVRKEDPAGFLNTIF
                     EREDCINHPALLAEALKTAGVFKRGDLVQKLPGFLTHADTQVVFWAIYCCARLGQPEA
                     VKQLKPFCVTTEENLAYLAPRALFLSMYFLPLETARNWIGEWVEQGFLRQAVQACGLL
                     GDPSAIPWLLEQMAVRSLNRIAAEAIFLITAQQPAQAPAPEAIDSEEDEHLDDESLFW
                     ADPKRCAESHAQGSYTTFNNFKAQIQSGNLREPFLAMRYQKMAQWLRT"
     gene            6622..7665
                     /locus_tag="PKZ52_01015"
     CDS             6622..7665
                     /locus_tag="PKZ52_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58373.1"
                     /translation="MPSHAYIAATGMITPVGPNTAATHAAVAAGISAYADSNHYNKQS
                     HPMRMALIPEDALPPLNPALNVLGLSYRQQRLLRIAVPALLEAVSKQPLNEPAALFLA
                     LPESIPGCDFKTDLNFIAALEIQSGVLLNKAASQIFTSGRAGGFEAIAAAMAYLSQNG
                     NSVALVGGLDTYLDLLLLGILDQNNRILAEQITDGFAPGEAAGFLLVISDQAKKYLPF
                     APAGIIYPPGIALEPGHRYSDQPYRGEGLANAWSQAIENANIGPIDKIFSSLNGENIG
                     AKEIGVALLRNKNAFAAENKIEHPADCIGDIGAAFAPVLVGLACASNTATRVLHSCSS
                     EQGKRAAAITVSA"
     gene            7765..8544
                     /locus_tag="PKZ52_01020"
     CDS             7765..8544
                     /locus_tag="PKZ52_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HNG58374.1"
                     /translation="MTDIGEAIAVIVATPDSDEPCWACKKEPETQLQNNLNESPSSIG
                     EPENQLHNDSSELGKSLGFRPTWTIQVAESAAPGAEPISAEVTAAAHHLIPGNASLKK
                     CPQILDLIEASRGKIRSDIGYNVNSEQNGVWLPGSYGVNPDSVFKKKWSAFTNQQEYA
                     FKAMDRANAQFHDAHPEYSRQVIRALRSLADKITLTAPEKCGICGEKTEDKARPPYGL
                     VGRLNQISSMHRRFLRGPMRKWPIQSGYFTSRWSGLRKNSL"
     gene            8650..9219
                     /locus_tag="PKZ52_01025"
     CDS             8650..9219
                     /locus_tag="PKZ52_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58375.1"
                     /translation="MYYKLDSQFPMTDDNTYFDISDGVEIDGIASWARGEPFTATPPN
                     PIVIDIEKIGDSDDLSLRPIAFNDANLCIAIPEIVDALIACGVNNIQTYPAVLRDVTT
                     QKEYPYCAINIIGLIKAADLSKSDWINLDGEARMDTVFLDLALDTDKIRSYDLFRLYE
                     DTGTIIISEKVKKALEHFPDLIFIPVGKK"
     gene            complement(9604..10800)
                     /locus_tag="PKZ52_01030"
     CDS             complement(9604..10800)
                     /locus_tag="PKZ52_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNG58376.1"
                     /translation="MHPHSSTKIVKFTSIFAALLSMNAVGQALNEAINVQLSAAVSPC
                     SNLLGADDGGAVLTGGLLDICGRSSPFGSVPNSQGASTANTQSLAGDARGLLQNARGD
                     TGVSQEITANWSFFANIEHETLNHDATALADGFDSDALRVNAGVNYAFSANNHLGIAL
                     VSKTHEGDYQAGGDFSSDSIGARLIADFGLTENTFVQVLLGRDNTDLDRSRKATFFDT
                     ADGAVIYSLSASPKSAYSGTETEAAAVLGGSWLFGGTTLSPTLGVNWQQIDYDTHSEA
                     GNSGLEVTTYANTADFLQAIIGLQASWAVSADWGVWAPQIGASVINEFENKSRQLQVS
                     FTGDTRAKRFTYDTEEGDSSYMTLSAGSVFLLKNGVQFYLNAEHYVGFADYSRTVLSG
                     GLRWEL"
     gene            11040..>16015
                     /locus_tag="PKZ52_01035"
     CDS             11040..>16015
                     /locus_tag="PKZ52_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNG58377.1"
                     /translation="MPRQTLATALIKSLVLTLMLSTATGCGKGGGGGGGGNNSPSNSS
                     SSSTSSSSSSSSSSSSSGSSLKPTAVLSFSTPSVNPTSLPPNTTTAVVFFTQVSQNLE
                     QLSHPLVLEEINAEGALLGALGNLADDGKDGDERRGDGVYSLRVSLEAGAANKRFFRI
                     STLFNDKNQYSNLIPLWVSDVATSTRAPNLSLTLTDTQTQQEVFANEVVIELAAGVLP
                     TQAALDKLVAVVGGKVLGCIPSLNRCLVEVPTTTLDGLNQVLTTLRQQLGIQDAYLSA
                     RTTAQDSAVSSNLPCTGTDCQWYLQRIRALEAWQIAGGGDKARGVAVIDFGVNCALSV
                     LGCTSSQTDTIDHGTGVAAFIAGKPDNSRGTAGIAWNTSLYPYNILKGDGSQYQISEL
                     ITRSLSEPQVRVINISAGTAIDIKHLLRDTLCQATAQGRLTVVAAGNPVPVEGKCEGA
                     FIYPARFAQESVCPNGADLKSSLLVVGATDQENKLAQWQAGAYTRCSNLLYSNIYAPG
                     QDILGLSSDNTWTLKTGTSYASPLVAGAAALVWASNPQLTAAEVRSRILDNANPLSLT
                     QADERLRTSDARLDGKPLLDVYQAVSGKPLPAQVDNTPAPVVWSAVANASNEGKTAPV
                     VIKDINVPVPIKVEGGFYSIDGGPFTDIAGKIEPGQSLQLMAKEDGPAAQSTPINVSI
                     GGQTQQVVVTALPVNSSGSSTSSASSSSSSSSSSSSSAASSSSSSSSSSSSSGELNAS
                     SSNGSSSSSSSSTSSSSSSSSSSSSSSSTSSSSSSSSSSSSSNSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSGKSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSSSSSSS
                     SSSSSSSSSSSSSSSGINAVPVVLQISLVDSNGGSVVAGDTLTGSYFYLDAENDIEGA
                     SQFRWILNGTAISGATSLTYLVKDSDVGKKLAFEVTPKALTGSMVGIATRSSEVVVSS
                     RGPVFTSAPTASIPENKTGIVYTASATDPDNDPLSYSISAGEDKNLFTIDSSTGAVYV
                     RNPPNFEAPADADANNIYRFTVAVSDGQGGVATQDVEISVTDVAILQPQITFPTAGAD
                     LMEAKTQITVSGNLIDTEDGIVSARDISSMTVNGTAVVIDGMVPSRWSVAIPISKGYS
                     TIRAEVKAANGVTSTVQQPVSNNHPFTNPSALALDGDFAYVIDGEKVLAANIVDGSRK
                     VISANYTSGRGTGPNLVNAYALAVDTVNKRLFVSSQSSSNSVLAIDIATGNRTAVAPY
                     GNGVPDGLTFDAANNRLFAVYSKSVAQINVSTGVITPISNDTIGTGTKFSQLSGHDGV
                     IYDADNNRLIVLEANRGKFVSVDIATGNRVDLNPTNVTSGKKAFAFDKANNRILMVAP
                     TTSTFNSAYSLYTVNATTGVYSIMTDLAAGSGPVIADPLSLAFDSNNKRVLVTKQSTS
                     ALYGINLTTNARSVITGNNTNDGAVFYGRYLDIDTPRNRVIGVDSLDSLSTLFSIDLS
                     SGKKSPISNATIGAGLKFGGIQAMQVDIYYNAAFVLDKSSLSTCSLVYV"
BASE COUNT         4092 a         4340 c         3864 g         3719 t
ORIGIN      
        1 tttatccctc agccttcagc caggggaagc attactcatt caaggcccct ccggcgttgg
       61 caaaaccacc ttattgcgcg cctgcgctgg cctttggccc tatgcacagg ggcagctgac
      121 tctgcccgag ggcagcagcc tatttctacc gcaaaaaccc tatttacccc tgggcacctt
      181 aaaagaagca ctgttttacc ccaactcgcc cagtgagcaa gctctggcgc cggttcttga
      241 gcaggtacag cttgggcacc tggtggcgcg cctggacgac gaggacgact ggggccgtgt
      301 attaagccta ggggagcagc agcgcctggc cttcgcccgc gccctgttaa ttcgccctgc
      361 ggtggtgttt ttagatgagg ccagctccgc tatggatgaa ggcttggagt atgccctgta
      421 ccagctgctg cgccaggcgc tgccgcagag tattgtggtg agcgtcggcc accgctccag
      481 cctgttgcaa ttccaccccc agcgcctgca cttaagcggt ggtggcgctt ggcagctgga
      541 aaccgcttaa acccctggtt gcactctgct caattgccgc tacctaaaat tgccgccttt
      601 acaaaggcaa tgtgattaag ccttaggggc aacccttgtg gttgccctat tgtgcctcag
      661 ctaacgattg agattggcgc gcccacaggc aaccacccgc ccctgcgggg cacccctcca
      721 gaggagggga attttaacca ctgcttgcgg cgcgtttagc gtgcagcaca cccctagaga
      781 ttgctcctcc gaacggggcg ggctgataaa cctaaggacc tatttttaca ggtatagggc
      841 ccgccaaaag ggttacaagc caggacttta ttgccatcaa tgaccggaga attgtttctc
      901 taatcccttc gggttggttt tccataattc acccaattcc gccgacatgg catccgggaa
      961 aatatcctcc aggccctgct ccatctgggt aaaaatattc tgggcggttt gcgcggcgga
     1021 ggttttcggc aggtcaaatt cacgggccat atcggtttca atggggccgg ggaaaatacc
     1081 aaacacggcg atatttttag gcgttaattc ggcgcgcata gcttgaatag cggaggccaa
     1141 agccgctttg gaggcgctat aaccgcccac gcccgccata ctggcaaggc cgataataga
     1201 gctaatactg gcgatggcgc cgtcaccatt gcgctctatt agcggtaaaa aagcccgcgt
     1261 tacagcgagg gtgccaaaat aatttacgtt catatcctgg tgtaaatcgg caaaatcacc
     1321 gcttaaaacg ctggcaaagg ccaatacccc ggcgttattc accaatacat tcacgtccgt
     1381 agcctgttgc gcagcggctt ggatggatgc ggcatcggta atatccaatt gcaaaggcac
     1441 cacacgggcg tcgttaaaag aaggcaagtt ggcaatcgtg cgggcgctgg cgtaaacctt
     1501 tttcgccccg aagcttaatg ccgcattcac taaggccttg ccaatgccgc ggttggcgcc
     1561 ggtaattaaa atggttttat tgtgtaggtt catgggtcgc tccaaagctt agttgatggt
     1621 tgcgcaggct cactcgctgg gtgctgttta tcaatcggcg taccgcaaat gctgcgttgg
     1681 gtggcagttt attgaagact taagaatcat taaatgctat tttcagcaag acattaatga
     1741 gcaataggca cgaatgaaca ctcaagccct gcaactattt gtagcagtgg cccgcagcgg
     1801 cagctttgcc gccgtggccc gccagcgcaa ccaagaccca tccctagtgt cccgcagcat
     1861 cgcgcaattg gaagcggagt taggcgtgcg gctgtttcag cgcagcacgc gcaaaatgct
     1921 gttgacggaa gcgggcgcac ggctacttga aaaggtgcaa gcactattgg aggaattaag
     1981 cggtgcatta gaggaggtgg caagttccgc ccatgaaccc tctggcacct tgcgcatgac
     2041 ggcatcggtt acctttggcc accactgcgt actgccctta ttaaaggaat ttcgcactct
     2101 gtacccgaaa ataaaaatag accttgtatt aagcgatgac ccattggatt taattgccag
     2161 ccacatagat ttggccatac gcctggggcc gtctattagc ggcaatttaa tcgtcacaaa
     2221 attgctcgac tgtcattatc gtgtggtggc aagcccggac tatatcgcgt catcagcgcc
     2281 gattgtgcag cctaaagatt taaccgcaca cgcgtgttta ttatttcctg agcgaatttt
     2341 tgcgccggat tggcaattta aacggcccgg ggaagagccc gtcgttgtac gcgtcagcgg
     2401 cgatttaatt ttatccacgg gcgatgcact gcggcagatg gcgctgcggg gtatggggcc
     2461 tgcactactg ccccactggg caattttgga cgcgctggag gatggccgtt tagtggattt
     2521 attcccgact tatcaagccg ccgcagcttc atttaatagc gcagcctggc tggtttaccc
     2581 aagccgggat tatttgccga aaaaggtccg cgtgatgatt gattatttaa aaactcacct
     2641 gccgagccgc tatgcaataa attctgtacc ggcataaatt gctgatcggc caatcaagaa
     2701 tacgcattta aaagacagtc ttgacgcagg tgaacactaa aaatagtgcc cggcaactgc
     2761 actacattta ttaaataaac gcatttaacc cgaccaggtt tccatccaat ccgcaccgta
     2821 ataaaaacct tttgccaagc ctaaattctg cgccttggcc acaaggctca ggggcaagcg
     2881 caggcgcact tgttcgtgca ggagtaattc ggcatgggcc gtatcgtgct caggcaaacc
     2941 aaatacatat tggccgaata atttccagcg cgtcatcaac tggccgcctt gggctgaggt
     3001 gctggcggac acaataaagc gatcaaaaat tcgcggtgcc cgatcgtctt tgtgctccag
     3061 caccaggctg ccatcggcaa aaataaaacc ccgttggcta ccattggctt tttccagtgc
     3121 catggcctcg ttgctggcca gaaattcctt tgcctcagta gttaaggcgg cgaagggtct
     3181 gcccgcttgg cgcaggccca gaatggtttt tttcatactg cggctacagg cttgcaccag
     3241 ttgcgactgg ccgaataaaa cccggctgcc agccaaaaaa tgcccgctgc cgccaacaaa
     3301 atgctgttgc agctggtgaa accatgtctc ttttaaatcc gccaggggcc gcaccgcctg
     3361 gcccaaccgt ttggcacagt ggttgtagac caggccccgc ccgccccaag ccgggtgggc
     3421 caaaagagcc gttaacacag cttgcgctgg cggcgttaaa gcatcctccg gcgtttggtg
     3481 cagggcttca atgatggcct gcagctcggt ttgatgactt accgagccat ccagtacaca
     3541 ggcgtccaat aaacccgtta acaactgcgg atcggtaaat acggggtggc ttaacagcgc
     3601 tgacataggt gtctcctgtt tatgtcgttg attaataaat gatgtgaatg gcgcgggcct
     3661 ttagcgggcg ggatgacagc tggcagcatt gcccgcataa tcggtgccaa tccgtctcgc
     3721 tgcccttttg gatcgcccat gcttcaactg ctcaaccaca ccccatttgc cgcccagatc
     3781 ggcctgtttc ccaatgagga gggtgtggat accttgtatg tagtggccaa ggcccacttt
     3841 aacatggcca atccgttaag cctctgcgat gaacaaacgc ccccgcgcat ggaggatgtt
     3901 tattacggcg aaccggggct ttctagcctt aaatacccca gcgattttca ccccggcaaa
     3961 gccgccaccg acgtgtttat gctcggccac gcctgctccc ccgacaaaca accggtgtat
     4021 tacctggata ccagcctaag cctcggcaac ctgcacaaaa ccattcgcgt ctggggtgcg
     4081 cgcacctggc aagggggcgc tttaagccag ccgcagccct ttacccatct gcccatcatt
     4141 tatgaaaacg cctacggcgg gcagcaggtg caggacgggc aagtagtcgg cggcgaagag
     4201 cgcaacccga tcggcaaggg ttttttaggc cgccaggagc gcagcacatt tgacggcacc
     4261 gccgcccccc agctggacga cccgtttaac cccctgcgcc aattgggtga tacaccggag
     4321 cccgcaagtt atggccccag agcgccaggc tggctgcccc ggcgggcatt tgctggcacc
     4381 tatgacgagg cctggcagca aaatcgcgcg ccgtttctgc cggaagacta ctcgccacgt
     4441 tttatgaaca gcgctcaccc agatctcatc gccgatggct ttttaagcgg cggcgaggcg
     4501 gtaaaaattc tcggtatgca ccccctaggc gagctgcagt ttcaaattcc caggctcagc
     4561 ttacacactg aaattaccat gggcgcccaa acccacagcc caaattttgt gttggagagc
     4621 atattgctag agcccaacca gctgcgcttt agtctcacct acaaagcccg cttaatctgt
     4681 gataaacagg ccctcaccat tcgccaagta gccctgcacc tgcagcgcta gtgtaaaaaa
     4741 aactgtaagg atttttatgc cacaaactac ttttgccaac atgcgcggca tcgtccacaa
     4801 gggctcaaac ggcatgagcc tagtatttcc cgacgtgtgc aaaacgccca cacccgcagg
     4861 ccctgtgccc atcccctacc ccaacctcgg caaatcttcc gacaccatga aaggccccac
     4921 ctcggtaaaa accgataaac aaatgccgat ggtgaaaggc gccgtgtaca ttatgagcac
     4981 cggcgatgaa gccggcagcg ccatgggcgt gatcagcaat aagattaaag gcgaatgcga
     5041 atttatgatg tattccttcg atgtgaaatt cgaaggcaaa aacgtctgcc gcatgggcga
     5101 ccctttattc cacaacaata aaaacatcat gggttaagcg tgaacgcacc ggttaatgct
     5161 tttgcacata tcatccaaca aatggccgac aacgccagct tcctatgggt actgcgcggc
     5221 cgtcagcggc gcgaacccca ggtccaccct gagcacatcg cagaattaga tgcgcgcatc
     5281 caagccaatt tagatgggct agtactctac ggcgaggacg cctgggccat agcccaagcc
     5341 aacggcgaat tcccccaagg aggcgaagca ttttgtttgg gttatttggc gtttagcagt
     5401 gaagatgtaa gtaaaatcca atacgccact gaatttgcct tgcaaaatga ggataccttt
     5461 aaaggcttgg taagcgccct agcctggcta cccggcctta aagtgcaccc ctggattacg
     5521 cagtttttcc acagcaaaaa cctcgaccac aaacgcctag cgctggcagc ctgccgcgtg
     5581 cgcaaagaag atccggcagg gtttttaaac acgatttttg aacgggaaga ttgtattaat
     5641 catccggctt tattagccga ggcgttgaaa acggcggggg tttttaagcg cggtgattta
     5701 gtgcagaaat taccggggtt tttaacccac gccgataccc aggtcgtttt ttgggccatc
     5761 tattgttgtg ctcggcttgg ccaaccagaa gcagttaaac aattaaaacc attttgtgtc
     5821 accacagaag aaaacctagc ctatctagca ccgcgcgcgc tgtttctgtc tatgtacttc
     5881 ttgccgttgg aaacagcacg caactggata ggcgaatggg tagagcaggg ttttctgcgg
     5941 caagctgttc aggcctgcgg gttgctcggc gatccttcgg cgatcccttg gttgctcgag
     6001 caaatggctg tgcgcagctt aaaccgaatc gcagcagaag caatttttct cattacagcc
     6061 caacaacctg cacaagcccc ggccccggaa gctatcgact cggaggaaga tgaacatctt
     6121 gatgatgaga gtttattctg ggcagacccc aagcgctgcg cagaatctca cgcccagggc
     6181 agctacacca cgtttaataa ttttaaagcc cagatacaaa gcggcaatct acgcgagcct
     6241 ttcctcgcca tgcgctacca gaaaatggct caatggctga ggacttaaat aaatttaaaa
     6301 gttaataaaa ccaatccctg cggacgattt attaaggaag ctctgaaaaa tctaggcggc
     6361 gaagcgctac taggcgaatt tgccaaaaaa agcgggcgac tgcagggagg caggacgacc
     6421 ccatggaagg gggaggtaga atttcatcgg gaatgaaatc gaggtagagc aatgcaggag
     6481 caattgccga gtttacacgc agtaaatgag tatttttttg gcaaattcaa cgacgccagg
     6541 gcgcgtaaga taattttttc agaggttcct taacaattca cccgtgctat tcagtacaac
     6601 catttaccca ggtcgcgaac tatgccaagc cacgcctata ttgccgcaac tggaatgatt
     6661 acgccggttg gtcctaatac agcagccact catgccgccg ttgccgccgg tattagcgcc
     6721 tatgctgatt cgaaccatta caataaacag tctcatccca tgcgcatggc attaattccc
     6781 gaagacgcct tgccacccct taatcctgcg ctgaatgtat taggccttag ttaccgccag
     6841 cagcgcttat tgcgtattgc cgtacctgcg ctgctcgaag cggtaagcaa acaacctttg
     6901 aatgaacctg cagcgttatt tttagcactg ccggaatcaa ttcccggctg tgacttcaaa
     6961 accgacctca actttattgc cgcgttggaa attcaaagcg gtgttttact aaacaaagct
     7021 gccagtcaaa tctttacctc aggacgcgcc ggaggctttg aagcaattgc tgcagccatg
     7081 gcatatttat cgcaaaacgg taacagcgtt gcattagtgg gcggcttaga tacgtatctg
     7141 gatttattac tactcggtat tctcgaccaa aacaaccgca tccttgccga acagattacc
     7201 gatggttttg cccccggaga agcggctggt tttttgttgg tcattagtga tcaagcaaaa
     7261 aaatatttgc cctttgcccc ggcgggcata atttatccgc ctggaattgc cctagaacct
     7321 ggccatagat atagtgatca accctatcga ggtgagggtt tggccaatgc ttggtcacag
     7381 gcaatcgaaa atgccaatat cggccctata gataaaattt ttagtagttt aaacggtgaa
     7441 aatattggcg ccaaagaaat aggcgtggca cttttgcgca ataaaaacgc ttttgcagca
     7501 gaaaataaaa ttgagcaccc agctgattgt ataggcgata taggtgcggc ttttgctcct
     7561 gtgttggtgg gccttgcctg tgcttccaac acggcaacac gggtgttgca ttcgtgttca
     7621 tccgagcaag gaaaacgagc agcggcaatc accgtgtccg cgtaacacag atacattcaa
     7681 tttaatttat attcgtcagt aaaaattcct gagaatattt cataattcaa tattacccac
     7741 gaaaccatta gcgaggatat agttatgaca gatattggcg aggctatcgc cgttattgtt
     7801 gccacacctg actctgacga accctgctgg gcgtgcaaaa aagaaccgga aacacaactt
     7861 cagaataatt taaatgaaag cccaagctcc attggggaac ctgaaaatca attgcataac
     7921 gattcgagcg aactggggaa aagccttggc tttaggccca cctggactat tcaggtggct
     7981 gaatcggccg ccccaggtgc ggagcctatt tctgcagaag taacggctgc agcccatcat
     8041 ttaattcccg gcaatgcgtc attaaagaaa tgcccgcaga ttctcgattt aattgaagca
     8101 agccgcggca aaattcggtc ggacataggc tacaatgtta actctgagca aaatggtgtt
     8161 tggttacccg gcagctacgg cgtaaatcca gattcggttt ttaagaaaaa atggtctgct
     8221 tttaccaacc aacaggagta cgcatttaag gccatggata gggccaatgc tcaatttcat
     8281 gacgctcacc cagaatatag ccgccaggtc attcgtgctt tgcgctcact tgccgacaaa
     8341 ataaccctaa ccgcacccga aaaatgcggc atctgcgggg agaaaaccga agataaagcc
     8401 aggccaccct atggtttagt aggccggcta aaccaaatct catctatgca ccgtagattc
     8461 ttgcgaggcc ctatgcgcaa atggccgatc caaagtggat actttacgtc gcgctggtcg
     8521 ggattgagaa aaaattctct ttagccttag ctgcacatag ttcaaaaccg attaaattca
     8581 ccccccaaca ttaaaaacaa ccgacaatgt acccccggtt attgacccac ttatctccag
     8641 gagcaaagaa tgtattacaa actagatagt caatttccaa tgacggatga caacacctat
     8701 tttgacatta gtgacggcgt agaaattgac ggcattgcaa gctgggcccg cggagaaccg
     8761 tttaccgcaa ctccacccaa ccccatcgta attgatattg aaaaaatagg cgattccgac
     8821 gacctatcct tacgccccat agcctttaac gatgccaatt tatgcatcgc aatccccgag
     8881 atagtcgatg ctttaattgc ctgcggcgtc aataatattc agacttatcc ggcagtgttg
     8941 agagatgtca caacccaaaa agaatatccc tattgtgcca ttaacatcat cggattaata
     9001 aaagcggctg acttatcgaa atccgattgg ataaatctag atggcgaggc taggatggac
     9061 actgtttttt tagacctagc actcgatacc gacaaaatcc gcagctatga tctatttaga
     9121 ttatacgagg acaccggcac aatcattatc agtgaaaaag taaaaaaagc cctcgagcac
     9181 tttccagatt taatttttat ccccgtgggc aaaaaataaa accagccgaa tattttctgt
     9241 acgcaccaca aagattcgac ttgtccgccg cgaaaatgct gcagcgtcat aactacacca
     9301 cggataaaac ccacttcgcg atacgtttat agctcgcgct attattttta gaggccatcg
     9361 cattagaaca taaattcgaa gcgctttatt tcccgctccc gcgcaatagc gtgggagcga
     9421 gttcgcgatg ctattgcaca ttggctacag tccctgctca aaccaaacag cctgtactag
     9481 cgagcaaaaa aacaccacgc cctcaatcac cgcagctgga atcggttacc tcacccagca
     9541 acccactcca ttaacctcag cgaatatgta aattcaatgc agccttacca gtaaaaaatc
     9601 ctcctacaac tcccaccgca atccaccgct caatactgtg cgactgtaat cagcaaaacc
     9661 cacatagtgc tcggcattta aataaaactg cacgccattt ttcaacagaa atacactacc
     9721 tgccgacagc gtcatgtaac tgctatcgcc ttcttctgta tcgtaggtaa agcgtttagc
     9781 gcgggtatcg ccggtaaaac tcacctgtaa ttggcggctt ttattttcaa attcgttaat
     9841 aacacttgca ccaatttgcg gcgcccatac accccaatcg gcagaaaccg cccagctagc
     9901 ctgcaagcca ataatcgctt gtaaaaaatc ggcagtattg gcataggtgg tcacttctaa
     9961 accggaatta cccgcttcgc tgtgggtgtc atagtctatt tgttgccagt taacacccag
    10021 tgttggcgat aatgtggtgc caccgaataa ccaactgccc cccaacacgg cggccgcttc
    10081 ggtttcagtg ccactgtaag cggatttagg gctagcactg agcgaataaa tcaccgcgcc
    10141 atcggcagta tcgaaaaagg tagccttgcg gctgcggtct agatccgtat tatcgcgccc
    10201 cagtaaaacc tgtacgaacg tattttccgt taaaccaaag tccgcaatta aacgcgcacc
    10261 aatgctatca ctggaaaaat caccgcctgc ttgataatcc ccttcatggg ttttactgac
    10321 taatgcaata cctaaatgat tgttcgcact gaaggcgtag ttcacgcctg catttaccct
    10381 taatgcgtcg gaatcaaaac catcggctaa tgcagttgca tcatggttca aggtttcatg
    10441 ttcgatattg gcaaaaaaag accaattcgc tgttatttct tggcttaccc cggtgtcgcc
    10501 ccgcgcattt tgcaataaac cacgggcatc acccgcgagg ctttgggtat tggccgtgct
    10561 tgcaccctgg ctatttggaa cactgccaaa gggcgacgag cgaccgcaaa tatcgagcaa
    10621 accaccagtt aaaacagctc cgccatcatc ggcaccgagc aaatttgaac agggcgaaac
    10681 tgccgcactc aattgaacat taatcgcctc atttaaagcc tgcccaacgg cattcatgct
    10741 taatagggct gcgaaaatac tggtgaattt tacaattttt gttgatgagt gcgggtgcat
    10801 gatgaatcct ctttaggggg cgtggtgcgg cataaaaccc ggcatgtaaa cggaatttag
    10861 atggcaatac aagctttgtg tgagaacagg gccgcccaga gcgcgtgctt atttcaataa
    10921 gtacgcaata agtggcgtcg atatttgact cctctcacag atggggcatg ggaaagatta
    10981 ttatttgcgg cagcttgcta aactgcgcac cactcaagat ttactgcgat ggaagcacca
    11041 tgccccgcca aaccctagcc actgccctaa ttaaaagcct tgttttaacc ctaatgctgt
    11101 caactgccac ggggtgcggc aaaggcggtg gcggtggcgg tggcggcaat aatagcccca
    11161 gcaactcgtc gagcagttct acatccagca gcagctcctc ctccagcagt agctcatcct
    11221 caggcagcag cctcaagccc acagcggtgt tgtccttctc taccccatcg gtaaatccca
    11281 ctagcctgcc gcctaatacc accactgcgg tagtattttt tacccaggtc agccagaacc
    11341 tagagcagtt aagccatcct ctagtgcttg aagaaataaa tgccgaaggg gcgctgctgg
    11401 gggccttagg caacctggca gacgatggca aggatggtga tgagcgccgt ggcgatgggg
    11461 tttatagcct gcgcgtaagc cttgaggccg gtgcggcaaa caaacgcttt tttcgcatca
    11521 gcaccttatt taacgataaa aaccaatact cgaaccttat cccgctgtgg gttagtgatg
    11581 ttgccaccag cacccgggcg ccaaacttaa gcctgacgct gaccgacaca caaacccagc
    11641 aagaggtgtt tgccaatgag gtggtaattg agctggccgc aggggtgttg cccactcagg
    11701 ccgcgctgga caaactggtc gccgtcgtcg gcggcaaggt tttgggctgt attcccagcc
    11761 tcaaccgctg cctggtggaa gtgcccacta ccaccttaga cggccttaac caggttttaa
    11821 caacactgcg gcaacagctg ggtattcaag atgcctactt aagcgcccgt acaacggcgc
    11881 aagacagcgc ggtaagcagc aacttgccct gcaccggtac cgattgccaa tggtatttac
    11941 agcgcatccg cgctttagaa gcctggcaga tagccggggg cggcgataaa gcccgcgggg
    12001 tggcggtgat tgatttcggt gtgaactgcg ccctgagtgt gctcggttgc accagcagcc
    12061 aaaccgatac gattgaccat ggcaccggag tagccgcgtt tattgcgggc aaacccgata
    12121 acagccgagg cacagcgggc atcgcctgga ataccagcct ctacccctac aatattctca
    12181 agggcgacgg cagccagtat caaatcagtg aattaattac ccgcagcctc agcgagccgc
    12241 aggtgcgcgt aattaatatc agcgcgggta ccgctatcga tatcaaacac ctgctgcgcg
    12301 ataccctatg ccaagccaca gcccaagggc gccttacggt tgtagccgcg ggcaacccgg
    12361 tgcctgtcga gggcaagtgt gaaggggcat ttatttaccc agcgcgtttt gcccaagaga
    12421 gtgtttgccc caacggagcc gaccttaaaa gcagcttgtt agtggtagga gccaccgacc
    12481 aagagaataa attagcccaa tggcaggcgg gcgcttatac ccgctgcagc aacttgctct
    12541 acagcaatat ttacgcgccg gggcaggata ttctgggcct cagcagcgat aacacctgga
    12601 cccttaaaac cggcaccagt tatgcctccc ccttagtggc cggagccgct gccctggtat
    12661 gggcgagcaa cccgcaactc accgctgccg aggtgcgcag ccgtatcctc gataacgcca
    12721 atcctttaag cctgacccaa gccgatgagc gcctgcgcac cagcgacgcg cgcctagacg
    12781 gcaaacccct gttggatgtg taccaagcgg ttagcggcaa acccctgcct gcccaggtgg
    12841 acaacacccc cgcccccgta gtgtggagcg ccgtagccaa cgccagcaat gaaggcaaaa
    12901 cggccccggt cgtcattaaa gacatcaacg tgcccgtgcc tattaaagta gagggcggtt
    12961 tttattccat cgacggcggc cccttcaccg atattgcagg caagatcgaa cccggccaaa
    13021 gcctgcaact tatggccaaa gaggacggcc cagccgccca gagtacgcct atcaacgtca
    13081 gtatcggcgg gcaaacccag caggttgtgg tgacggcgtt gccggtgaac tcatccggtt
    13141 caagcaccag cagcgcgtcc agctccagca gttcttcatc aagttctagc agctctgcag
    13201 cgtctagctc atcaagctcc agtagctcat caagctcgtc gggagaactc aacgccagtt
    13261 catcgaatgg ctctagcagt tcatcgtcaa gctcgaccag ctcttcaagc tctagcagct
    13321 cttcctcaag ctcaagcagt tcgaccagct ctagttcatc gagcagctct agcagttcgt
    13381 cttcgaattc atctagctct tcatcaagct ctagttcgtc aagcagttca agctcaagca
    13441 gctcgtcaag ctccagtagt tcatcgtcca gctctagcag ctcatccgga aaatccagtt
    13501 ctagctccag ttcgtcaagc agctcatctt cgagttcatc gagttcaagc tcaagcagct
    13561 cttcatcaag ctctagctca tcaagttcca gcagttcatc atccagctct agcagctcct
    13621 caagctcttc ttccagctca agttctagtt cgtccagtag ctcctcaagc tccagcagtt
    13681 catcgtccag ctctagcagc tcctcaagct cttcttccag ttcaagctct agttcgtcca
    13741 gtagctcctc aagttccagc agttcatcat ccagctctag cagctcctca agctcttctt
    13801 ctagctcaag ctccagttcg tccagtagct cctcaagctc cagcagttca tcgtccagct
    13861 ctagcagctc ctcaagctct tcttctagct caagctctag ttcgtccagc agttccagct
    13921 caagtacctc gtccagctcc agcagttcat cttcaagctc aagctcctcc tcaagttcca
    13981 gctcatcgtc tagctcatca ggcatcaatg cggtacctgt agtgctgcag ataagcctgg
    14041 ttgatagcaa tggcggctct gtagttgcag gcgataccct tacgggttct tatttctacc
    14101 tagatgctga aaacgatatt gaaggcgcca gccaatttcg ctggattctg aacggcacgg
    14161 cgatcagcgg ggcaacctcg ctcacctact tagtaaagga tagcgatgtc ggcaaaaagc
    14221 ttgcgtttga agtcaccccc aaagcgttga cgggcagcat ggtgggtatt gcaactcgct
    14281 caagcgaagt agtggtgagc agccgcggcc cggtatttac ctctgctccc accgcgagta
    14341 ttcctgaaaa taaaaccggt attgtctata ccgcttcagc gacagatccc gataacgacc
    14401 cattgagtta ttccatcagt gcaggcgagg ataaaaatct atttactatt gatagctcca
    14461 ctggcgcggt gtatgtaaga aacccaccga attttgaagc tccagcggat gccgacgcta
    14521 acaatattta ccgatttact gttgcggtaa gcgacggcca aggaggagta gccacgcagg
    14581 atgtagaaat ttccgtaacc gatgtcgcta ttttgcagcc gcaaataacc tttcctactg
    14641 ctggcgcaga cctgatggaa gcaaaaacac aaattaccgt atctggtaat ttaatcgata
    14701 cggaagatgg catcgtcagt gcgcgagata ttagttcgat gacggtaaat ggtaccgctg
    14761 tggtcatcga tggcatggtt ccatcgcgat ggtctgtagc aattcccatt tctaaaggtt
    14821 atagcactat tcgcgcggaa gttaaggctg caaacggtgt taccagtacg gtgcagcaac
    14881 cggtgtcgaa taaccacccg ttcaccaacc catcggcgct ggcactagac ggtgattttg
    14941 cttatgtaat tgatggcgaa aaagtgcttg ccgcgaatat tgttgatggc tcacgaaaag
    15001 ttatatcggc taattacacc tcgggacgag gcaccggacc taaccttgta aatgcctatg
    15061 ctcttgcagt ggatactgtt aataagcgcc tgtttgtatc tagccagtcc tcatccaata
    15121 gcgtgttagc cattgatata gccacgggga accgcacggc ggtagctcca tatggcaatg
    15181 gggtcccgga tgggttgact tttgatgcgg caaataaccg attattcgct gtttacagta
    15241 aatcggtcgc acaaataaat gtcagcacag gcgtaattac acctatttca aatgacacca
    15301 taggaacagg cacaaaattc tcacaattaa gcggccacga tggggttatc tatgatgccg
    15361 ataacaaccg gttaattgta cttgaagcaa atcgtggcaa attcgtaagc gttgatatag
    15421 ctacgggtaa tagggtcgat ctcaacccaa ccaatgtaac cagcggcaaa aaagcgttcg
    15481 cttttgataa agccaataat cgaatattaa tggtagcgcc aaccacatcg acatttaatt
    15541 ctgcgtatag cttgtataca gtcaacgcca ctaccggtgt gtattcgata atgactgatc
    15601 ttgctgctgg atctggtccg gtgatagccg atccattatc gctggcattt gatagcaaca
    15661 acaagcgcgt attggttacc aaacaaagca catcagcact ttatggaatt aatttaacaa
    15721 caaatgcgcg cagcgttata actggcaata acaccaatga cggcgcggta ttttatggcc
    15781 gttatttgga tatcgacaca ccacgcaata gagttattgg tgtagacagt ttggactcgt
    15841 tatcaacttt attttctatc gatttgtcat cggggaaaaa gtcgccaatt tcaaatgcca
    15901 ctattggcgc aggcctaaag tttggtggca ttcaagcgat gcaagtagac atttattaca
    15961 atgctgcgtt tgtactagat aaaagctccc tgagcacctg ctcattggtg tacgt
//
LOCUS       DAOQGS010000013        15762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1704_length_15762_cov_4.717005, whole genome shotgun sequence.
ACCESSION   DAOQGS010000013 DAOQGS010000000
VERSION     DAOQGS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1704_length_15762_cov_4.717005"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1351)
                     /locus_tag="PKZ52_01040"
     CDS             complement(59..1351)
                     /locus_tag="PKZ52_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNG58378.1"
                     /translation="MPIAPQDDKPTKALTSRAIEAMKPASKELADSGENRGLRVTCGA
                     KGIKTFVYRYRSPLTRKLVQISIGHYPTVSLAEARLKCSELKLIRANGRCPATEQRER
                     AAVEAKINQEKNLSATFTVRDMVELYLTQHIEDRKVDGRVIPGARKSKGQSETRRTLY
                     GDAVRVLGDHPAANITRKDITSMVMAIIERGANVQAGSVLRELSAAYEYAIGLEKLPD
                     TFANPALLAKAGLRQAKIKLTSERGKRVLSEAELKKLLQWLPGSAYTTTQKNILRFTL
                     WTGCRTGEVCAAEWRDIDLDKKTWHLRDTKTGVERYVQLSHQAVEFLKVLSLTTGDYL
                     FPSIKGKKPLQQKQLTEQAWRLRTDGRMLDIEPWTPHDLRRTVRTGLSRLGCPNEIAE
                     AILGHSRSGIEGTYDLHKYEKECAHWLQVWADFQSNLL"
     gene            1561..4188
                     /gene="mutS"
                     /locus_tag="PKZ52_01045"
     CDS             1561..4188
                     /gene="mutS"
                     /locus_tag="PKZ52_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNG58379.1"
                     /translation="MNAIEPTDQHTPMMQQYLRIKAQHSTELVFYRMGDFYELFFDDA
                     KTASRVLDVTLTARGKTAGEPIPMCGVPYHAAESYIGKLVRNGYSIAICEQIGDPATS
                     KGPVERQVMRIVTPGTVSDEAFMDAGRDNLLVALYPAAEGFGLASLDMSSGRFSLMEL
                     KDSQEVQAELERLRPAELLIPDTFNNEQLALPVRGVRKRPIWEFELDQARRQLCQHFA
                     VRDLIGFGCDEAVQALCAAGALYSYAKETQRTHLGQIQSLQLESLAQTVAMDSATRRN
                     LEIDQNLKGGEEFTLFAQLNTCVTPMGARLLRRWLHQPLRDADKLTQRQDAIAALLAN
                     YQYEALRNELKPIGDLERILGRVALRSARPRDLSRLGQSLAALPSLLQLLHPLRSAPI
                     SALATQVGQYPALAELLQRALVENPPVVIRDGGVIAEGFDAELDELRSISTNAGDYLV
                     RLEQQEKARTGLSTLKVGYNRVQGYFIELSRGQAEQAPADYIRRQTLKGTERFITPEL
                     KAFEDKALSAKSRALAQEKQLYDELIERINEDLLPLQTTSNALCELDVLSSLAERAET
                     LRLCRPSFTTTAGINIREGRHLVVEAVLDTPFVANDLNLNRDQPMLIITGPNMGGKST
                     YMRQTALIVLMAQIGSYVPASEAHLGLVDKIFTRIGSSDDLAGGQSTFMVEMSETANI
                     LHNATDNSLVLMDEVGRGTSTYDGLSLAWACAEYLAQRVRALTLFATHYFEITRLPER
                     LPQVGNVHLAATEHQDRLVFLHHIQAGPASKSFGLQVAKLAGIPAAVIDSAKAQLAIL
                     EGTPTPVLSSSESPVATIEDNAVAEPKARRHKQVQRDIFSSAGKLDAYIAAINPDKLT
                     PLDALKLIYKLKEELELTA"
     gene            4298..4621
                     /locus_tag="PKZ52_01050"
     CDS             4298..4621
                     /locus_tag="PKZ52_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNG58380.1"
                     /translation="MTFVVGENCIKCKHTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVDAIFSEDELPEDQEVFLELNAELAQVWPNITEKKESPEDAEEWDGVPGKI
                     KYLQR"
     gene            4826..5098
                     /gene="rpsT"
                     /locus_tag="PKZ52_01055"
     CDS             4826..5098
                     /gene="rpsT"
                     /locus_tag="PKZ52_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNG58381.1"
                     /translation="MANTPQAKKRARQNDKARLHNASLRSMVRTYIKKVVNAIATGDA
                     QVAKAAYELAVPVIDRIADKGIIHKNKAARHKSRLNAQIKALSQAA"
     gene            complement(5460..5603)
                     /locus_tag="PKZ52_01060"
     CDS             complement(5460..5603)
                     /locus_tag="PKZ52_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58382.1"
                     /translation="MNSAKQAAARRNSLLLTAAACSADRRCGIPATAIHPAKFNPAQK
                     AAG"
     gene            5688..7265
                     /locus_tag="PKZ52_01065"
     CDS             5688..7265
                     /locus_tag="PKZ52_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HNG58383.1"
                     /translation="MRTLLLGTIGTLTAIGAPLSWAAAPTANNDFRTISVGASVTINA
                     LNNDSDPDGDLIRIASIGRPNVGTATLRQQDSAIVFTAPADFKGQVTFTYTLEDIPNA
                     STETAPQQTTGTIVINVVAFAGSTAGSNQQSVGQALVKACDALDSANSNTLAQGQLEL
                     KQRCAGLVYLQINNPSGLDEVVRQISPEETLALAKAATGATEAVSAALGKRLSSMGSG
                     NTFSLNGKSWERETGGAAGDDLRSSPWGLFATAQIETADKDRTQFENGFNYSANSLLV
                     GVDYRLSSDWVIGLAGGFGKNDMDFLNKDGNVTTDTQSAIFYHLLNFDAFSWDVQLGI
                     SNSSYDINRRIHYNDTQANEWTSAASTSGQQTFATTTLQYQFSYRALSLFPSLKVGLT
                     QSTLDAYGENNLGGYEVSIGEQKEKQTKIEAGLQGQYALNFSWGVLTPTASANFISRA
                     SSSKDPVTGTFAYGPLSEANFVMTPEEFDSSYYQVSLGTSAVFPNGFSAFITAQQTLG
                     YENFSSQQYSLGVRIEL"
     gene            complement(7747..9723)
                     /locus_tag="PKZ52_01070"
     CDS             complement(7747..9723)
                     /locus_tag="PKZ52_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HNG58384.1"
                     /translation="MKKFFLFNLLLLCLCACAQHQTPATQFKARADQATPVTALGSPD
                     GSIQVELVLSSEGRLSYSIRRHGQMVVLSSGLGLVLHHNEFVEGLSLVQIADIQPLQE
                     SYLLKHGKKHAVNNAANERVVRLRNKENNTLEVIFRVFNDGIGFKYRALAHGGAPQHF
                     LTELSQFTLPADAKAWLQPIAPAQTGYANTNPSYEEYYQMDIAADGAPVSAAGWAFPA
                     LFRSGDTWLAITEAGMDGHFHASRLQTDATSPGRYLIGQPSAPEVFTGGALLAPDAPI
                     IETPWRIIAMGSLKNLVESTLGTDFALPAKANMDFVQPGVVAWSWALLKDEGTRYNTQ
                     RAFIDYAARMHWPYVLIDALWDSQIGKLQMAQLAQYAASKGVGLILWYNSSGAWNTTP
                     QTPKNILIDRSRRLEEFAWLQRIGIKGLKVDFFAGDGQSMLAYYTQLAEDAAQFNLML
                     NYHGATLPRGLQRTYPNILTMEAVKGLEFITFSQAAADAAPSHMAMLPFARNLFDPMD
                     FTPTVLSSIPGINRRTRVGFELAVSVLFLSGWQHIAETPAGMAKVPHYVKNILRDLPV
                     SWDETRFVDGFPGRYAVIARRKGNQWYVAAINGQPNSIDLNLNLDFVGNQKGELIGDG
                     MQSNQFDRRIIKPGQAVSLALKPFGGFLIKYAEE"
     gene            complement(9942..10325)
                     /locus_tag="PKZ52_01075"
     CDS             complement(9942..10325)
                     /locus_tag="PKZ52_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNG58385.1"
                     /translation="MQQPTENPDSSLEMRINRRVGRGHHRNSLFNTCHLDEGEWTLMA
                     PGVEFKQAHTCARHDTYLWRLAPDTRLPAHHHDTDEESILISGDIWLDGEYCEPGAVH
                     FAPQGSSHQSIFSAQGALLVVKRYK"
     gene            complement(10339..10899)
                     /locus_tag="PKZ52_01080"
     CDS             complement(10339..10899)
                     /locus_tag="PKZ52_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNG58386.1"
                     /translation="MPELDTANCSDAALAAAIKERDPRALHQLYQLMSGKLLRLALRI
                     TQDKGAAEEVVEDVFVHAWQHMEQWQAHRGSLVAWLSMLCKSRALLYWRQPHAQKWDL
                     FADTPEYLEEGSAQADPFEWNIQLFAGPLKKAIAQLSPPEQQVLGALYRLGLTHEETA
                     QATGLPLGTVKSHARRAMARLQQSLV"
     gene            complement(10977..11396)
                     /locus_tag="PKZ52_01085"
     CDS             complement(10977..11396)
                     /locus_tag="PKZ52_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58387.1"
                     /translation="MPLSIKFSTHCLAALATSMLLVVPAHASSIRGPISFGPDARVKI
                     ECPGQPAFNNAVQSEVVGFGNFAQRVNIKLLYRPDLSADFNLVPGTDKYTKDYFVALN
                     SYLGNFPGPGYYAINMRNNNSFVVNLPANEEFGINCR"
     gene            11912..13597
                     /locus_tag="PKZ52_01090"
     CDS             11912..13597
                     /locus_tag="PKZ52_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNG58388.1"
                     /translation="MFFTNKPLWTPVLITGFLTMGLTACGSGSKSNPGPSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSGAALAANLLTSDGSFASGKMNFSDNPTDATLTYNGEFKA
                     VINKVSTESWHVQLTHGLAIKQSTTYTICFDAKAEAARSITFDIDAAGTPGYDSLIGS
                     QTAALTSDYQTIKYTFAATSTDDTARLAFNMGKSDVDVYLDNIGVFEGNNCGSFAAAP
                     LPGTPPIALAESGLIGNLANGKTQYDSKCAGCHGVAGAGASAPALNTNAVVRTKNGTA
                     YSLEGYVAAFMPKSAPGTCSGQCAADVAAYVKAGFNVPSKTTCSAGTGVICEDFEEAS
                     LNSRWTSVGTTELTVEEKSSGTKSLKISAIGGKVNFNGVDLDLSKFTGLAKNHYGRVM
                     LKTPAVSENAGDFTFIEASGPANVLATTRLAETNRAIYRARVDGNHESFMGNYDTSPY
                     GAPLTDCWSHADYVAVPKNTWACFEWRFDATTNTLTYWLNGTKLGMSVKDKATGSGAV
                     AGCAGHPLDDIWSGPASFDKLHIGIDQYHSTSKARTLYIDDVVVDTERVNCPN"
     gene            complement(13725..13883)
                     /locus_tag="PKZ52_01095"
     CDS             complement(13725..13883)
                     /locus_tag="PKZ52_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58389.1"
                     /translation="MSNLLIRLSVGVIFYNDYFDGRHTANLLAYLNEARIISYKLGYC
                     VGEAVQDF"
     gene            complement(13911..>15762)
                     /locus_tag="PKZ52_01100"
     CDS             complement(13911..>15762)
                     /locus_tag="PKZ52_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58390.1"
                     /translation="SAKYPRSEDRPDFTANVFPRCLAVITCLIPGIALSAAIIDGQGG
                     EYVWGVLAALILSSVLFLVIIVFRRELNERLRTKMGVRKSADPDTSPVFVWLSLGMTG
                     VSTLVAWIAPLWLGATLGTVPTLMLGLAGVVCVGSYLVFLTRKSSFPTVTFLVFTSVI
                     LSSCTPYYIRLSAPYELHQDNRQPGDTRPKLIDEFTRWKGLQGEKSKTLIVVAASGGG
                     ITAAYWTSSLLATFQDIDPEFAPRLFLISSVSGGSVGAAVFTQALTCEMNSADMRKKI
                     AAVLGEDLLTPVLGTALFTDMFFWAPGPSILAHYEVKPMDRAIVFETRLEEMWREHFS
                     EQKECSGLDQDFLKQRSLTGKWQPILMLNSTHQETGRPVIQSQVKLADEDLSVGRPFE
                     TAIDLFSVARHDLRISTAAHNSARFPLVSPAGQIRPEPGKPALKLGRLIDGGYFENDG
                     AYTLMQSLIALGNRLDNMQVVVIQISIDSPRPFEPDYKTAQPEFIADQVARAPHVAND
                     LLSPVFGFFVARDSHQEDVRDRLRAWTLARGQNGVYLHFRALIPEERRDKPDPVKPPL
                     GWRLSGGSQEQLLNRTLCLDRNYKEWEKLAPVLGDNKDKVQLCGPSKADY"
BASE COUNT         4038 a         4214 c         3773 g         3737 t
ORIGIN      
        1 gacttccgtt aaagagctct attcagagaa aaataagctt cttacatttc taaaaaatct
       61 atagaagatt gctctggaaa tccgcccaca cctgcagcca atgagcgcat tccttctcat
      121 atttgtgcaa atcataagtt ccctcaattc cactacgaga atgccccaaa atcgcttcag
      181 ctatttcatt cggacaaccc aaccgggata aacccgtacg cacagtgcgg cgcaaatcat
      241 gcggagtcca tggctcaata tccaacatac gtccatcagt tcttagccgc catgcctgct
      301 ccgtgagttg tttttgttgt agcggctttt tccccttaat tgatggaaac aaatagtcgc
      361 ccgttgttaa gctcagcact tttagaaatt cgacagcttg atgggataac tgtacatatc
      421 gctccacacc cgtttttgta tctcgcaggt gccaagtctt tttatcgaga tctatatcac
      481 gccactccgc cgcacaaact tcgccagtgc ggcagcctgt ccatagggta aatctgagta
      541 tatttttttg tgtagtggta taggctgagc ctggcaacca ttgcagtaat ttttttaatt
      601 cggcctcaga taaaacccgc tttccgcgtt ccgacgttaa tttgatcttt gcctgtctca
      661 accctgcttt tgccaataag gcaggattag caaatgtatc aggcagtttc tccaaaccaa
      721 tcgcatactc ataggcagca gacaattccc gcaatacgct accggcttga acattcgcac
      781 ctcgctcgat aatcgccatt accatcgagg tgatgtcttt acgagtgata ttcgcagcag
      841 gatgatcacc caacactctc actgcgtccc cgtagagggt gcgcctagtt tcactctgcc
      901 cttttgactt gcgggcaccg ggaataaccc gcccgtctac tttgcgatct tcaatatgct
      961 gcgtcaggta gagctcaacc atatctctta cagtaaaagt ggcggaaagg tttttttcct
     1021 gattgatttt ggcctctact gcagcacgct cacgctgttc agtagcagga caacgtccat
     1081 tggcgcggat caactttaac tctgagcact tgagacgagc ctctgccagt gagactgtgg
     1141 gataatgccc gatacttatt tgcactaatt ttctggtgag tgggcttcga taacgataaa
     1201 cgaaggtttt aatcccttta gcgccacacg ttactctgag gccacgattc tctcccgaat
     1261 cagctaattc ctttgaagca ggcttcatgg cctcaatagc tctactggtt agagctttgg
     1321 taggcttatc gtcttgaggg gcaataggca taattagtcc aagtctcaaa attagaaaga
     1381 aacatttaga ttagtatagg ttgtttgttc ttaggttagt atagctcaaa caacctacac
     1441 tttgacctat actaatttat gcgattacgg ggtgcatcag ggtatttcat ggtgtatcaa
     1501 ccgcctcacc aaaaattaaa tttaaacaat taaatcagca agttaaaaga taaatactca
     1561 atgaacgcaa tagagccaac agaccaacac acacctatga tgcagcaata cctgcgcatc
     1621 aaagcccagc acagcaccga gctggttttt tatcgcatgg gggattttta tgaattgttc
     1681 tttgacgacg ccaaaaccgc ctcccgcgta ttggatgtaa ccctcacggc gcggggtaaa
     1741 accgcgggcg agcctatccc catgtgcgga gtgccctacc acgcggcgga atcctatatc
     1801 ggcaaactgg tgcgcaacgg ctattccatc gccatctgcg aacaaatcgg cgacccggcc
     1861 accagcaagg gcccggtgga gcgccaggtg atgcgcatcg tcacccccgg caccgtcagc
     1921 gacgaagctt ttatggacgc aggccgggat aatttattgg tggcgctata ccctgctgcg
     1981 gaagggtttg gccttgccag cctggatatg agcagcggcc gcttcagcct tatggaatta
     2041 aaagacagcc aagaggtgca ggcggagctt gagcgtttgc ggccagcgga attgttaatt
     2101 cccgacacct tcaataatga acagctcgcc ctgccggtgc gcggcgtgcg caagcggccc
     2161 atttgggaat ttgagctgga ccaggcccgc cgccagctgt gccagcattt tgcggtgcgc
     2221 gacctgatcg gctttggctg cgatgaagcc gtccaggctc tctgcgccgc aggtgctttg
     2281 tacagttacg ccaaagaaac ccagcgcacg cacctgggcc aaattcaaag cctacagctg
     2341 gaaagcctgg cgcagactgt tgccatggac agcgccaccc ggcgcaattt ggagatagat
     2401 cagaacctaa aaggcggcga agaatttacc ctctttgccc agctcaatac ctgcgtcacc
     2461 cccatgggcg cgcggctgct gcgccgctgg ctgcaccaac ccctgcgcga tgccgacaaa
     2521 ctcacccagc gccaggacgc catcgccgcc ctcttggcca actaccaata cgaagccctg
     2581 cgcaacgagc ttaaacccat cggcgattta gagcgcattc taggccgcgt ggccctgcgc
     2641 tcggcccggc cacgggatct ctcccgctta ggccaatccc tcgccgccct gccgagccta
     2701 ttacagcttt tacatccgct aagaagcgcc cccatcagcg ccctggccac ccaggtgggg
     2761 cagtacccgg cccttgccga gttactgcaa cgcgccctgg tggaaaaccc gccggtggtg
     2821 attcgcgacg ggggcgttat agccgaaggc tttgatgccg agctggacga gctgcgctcc
     2881 atcagcacca acgccggaga ttatttggtg cgtttagagc agcaggaaaa agcccgcact
     2941 gggctttcca ccctcaaggt gggctataac cgggtgcagg gctactttat agaactctcc
     3001 cgcggccaag ccgaacaggc cccagccgac tatatccgca ggcagaccct aaagggcacc
     3061 gagcgcttta tcaccccgga attaaaagcc tttgaagaca aggcactctc agccaagagc
     3121 cgagccttag cgcaggaaaa acagctctac gatgagctga tcgagcgcat caatgaagat
     3181 ctcttgccat tgcaaaccac cagcaatgcc ctgtgtgaat tggacgtgtt aagcagcctc
     3241 gccgagcggg cggaaactct gcgcctgtgt cggccgagct ttaccaccac agcgggcatt
     3301 aatattcgcg agggccgcca cctggtggtg gaagcggtgt tagacacccc cttcgtagcc
     3361 aatgatttaa acctcaaccg cgaccagccc atgctgatca tcaccggccc caacatgggc
     3421 ggtaaatcca cctatatgcg ccagaccgcg cttatagtgc tgatggctca aataggctcc
     3481 tatgtgcccg cctcagaagc ccatttgggc ctggtggata aaatatttac ccgcataggt
     3541 tcatccgatg acttagcagg cggccaatcc acttttatgg tggagatgag cgaaaccgcc
     3601 aatattctgc ataacgccac cgacaacagc ctggtgttaa tggatgaagt gggccgcggc
     3661 accagcacct acgacggcct ctcactagcc tgggcctgcg ctgaatacct agcgcaacgg
     3721 gtgcgcgccc tcaccctgtt tgccacccat tattttgaaa ttacccgcct gcccgagcgc
     3781 ttaccccaag tgggcaatgt gcacctagcc gccacggagc atcaggatcg tctggtgttt
     3841 ttacaccaca tccaagccgg gccggccagt aaaagttttg gtttacaggt ggcaaaactt
     3901 gccggcattc ccgccgccgt gatcgacagc gccaaggcgc aactggccat tttagaaggc
     3961 acgcccacgc cggttttaag tagcagcgag agtccagttg cgactattga agataacgcg
     4021 gtggcagaac ccaaagcccg ccgccacaag caggtgcagc gagatatttt ctcctccgcc
     4081 gggaaactcg acgcctatat tgccgcgatt aacccggata aattaacccc gctggatgca
     4141 ctcaaactta tctacaaatt aaaagaggag ctggagctga cagcgtgaat tgccacaata
     4201 gcttttcaag cctaggcttg cttgcttaca atgcccgccg attcacaatc gacacctatt
     4261 tgtgccctgt gccacttaag cttacgaggt ttataaaatg acatttgttg tgggtgaaaa
     4321 ctgcattaaa tgcaaacata cggattgcgt tgaggtttgc cccgtagact gcttctacga
     4381 aggccccaac tttttagtga tccacccgga cgaatgtatc gactgcgccc tatgtgagcc
     4441 tgagtgccca gtggatgcta ttttctccga ggacgagctg cccgaagatc aagaagtctt
     4501 cctggagctc aacgccgaat tagcccaggt atggccgaac atcaccgaga aaaaagaatc
     4561 ccccgaagat gctgaagagt gggatggcgt gccgggtaaa attaagtacc tgcagcgcta
     4621 gaggttaaca gcctgagccc tgcgcaaaag tgcggccggg tgctcaggcc cgccctgccc
     4681 ctgcggtaaa aagttgacag cagaggcgaa acgcggataa taccgcgtcc ctgattgggc
     4741 ctagccgctt tttgcgaaat caagcagccc ttaatcggcc caaacaacga attttcacac
     4801 tcattttttt gtaggagcac ctgcggtggc caacactccc caagccaaaa agcgcgcacg
     4861 tcaaaacgac aaagcccgtt tacacaatgc aagcctgcgt tccatggttc gcacatacat
     4921 caaaaaagtc gttaacgcga ttgcaactgg cgacgctcaa gttgctaaag cggcctacga
     4981 attagctgtc cccgtgattg accgtatcgc cgataaaggc atcattcaca aaaataaagc
     5041 tgctcgccac aagagccgtt taaatgcaca aattaaggct ctaagccaag cagcttaatt
     5101 cacaccgctc aggtcgaatt aaactcaata aaaaagctga ctatgtcagc tttttttttc
     5161 gcctgttgtt tttaggtaac tatttttagc gcagcttatt tcggcacagt ttatttcagc
     5221 acaccttatt ttaccttcta agcgcgggcg gcaaattacg gcagatttaa ccaagcggca
     5281 gaatcgatcg cggtgcagca gtgatagatg ggatctgtat tcatcactgc gggggagtta
     5341 ttgagggctc acaacactca cgcattcgcc tggtgattgc tatgcgacac gagaaccacg
     5401 tccagccaca acaaaatagt gctgaataca tatgttttta catattaatt gccaactgat
     5461 taaccagcgg ctttttgtgc cgggttaaat tttgccgggt gaatagcggt ggcagggatg
     5521 ccgcaccggc gatcggcgga gcaagccgcc gcggttaata acaggctatt tctgcgcgcc
     5581 gccgcctgtt tggcactatt cacatagcct tttgcttgtc aaagggcgca aacacaccta
     5641 gcatccgccc ccattcccac ttttaattat ttaacacaga ggctcccatg cgcacactcc
     5701 tactgggcac catcggcaca ctcaccgcta tcggcgcccc cttaagctgg gcggcggccc
     5761 ccaccgccaa caatgacttt cgcaccatca gcgtgggcgc cagcgtcacc atcaacgcac
     5821 tcaataacga ctccgacccc gacggcgacc tgattcgcat tgccagcatc ggcaggccca
     5881 acgtgggcac cgccaccttg cgccaacagg acagcgccat tgtcttcacc gcaccggcgg
     5941 attttaaagg ccaggtcacc ttcacctaca ccctagaaga catacccaac gcctccaccg
     6001 aaaccgcgcc ccagcaaacc accggcacca tagtgattaa cgtagtcgcc tttgccggta
     6061 gcactgcggg cagcaatcag cagtcggtgg ggcaggcact ggtaaaagcc tgcgacgcgt
     6121 tagacagcgc caacagcaac accctggccc aaggccagct ggaattaaaa cagcgctgcg
     6181 caggtttggt ttatttacaa attaacaacc cgagcggcct tgatgaagtt gttcggcaaa
     6241 tttccccgga ggaaaccctc gccctcgcca aagctgccac tggcgccacc gaagccgtta
     6301 gcgccgccct cggcaagcgc ttaagctcca tgggcagtgg caatacgttt agccttaatg
     6361 gcaagtcttg ggagcgagaa accggcggtg cagccggaga tgatctgcgc agttcgccct
     6421 ggggcctatt tgccacggcg caaattgaaa ccgccgataa agaccgcacc caatttgaga
     6481 acggctttaa ttattccgcc aatagtttat tagtgggcgt tgactaccgc ttatcctcag
     6541 actgggttat tggccttgcc ggtggttttg gcaaaaatga catggatttt ctcaataaag
     6601 acggcaatgt caccaccgat acccaatccg ctatttttta tcacctgctt aatttcgacg
     6661 cctttagctg ggatgtgcaa ttaggcatta gcaatagcag ttacgatata aaccgccgca
     6721 ttcattacaa cgacacccaa gccaatgaat ggacatcagc ggcctccacc agcgggcaac
     6781 aaacgtttgc gactaccacg ctgcaatacc aattctccta ccgcgcgtta agcctatttc
     6841 ccagcttaaa agtgggccta acccaaagca ccctcgatgc ctatggggaa aataacttag
     6901 gtggttatga ggtaagcatt ggcgagcaaa aagaaaaaca aaccaaaatt gaagcgggcc
     6961 tgcaggggca atatgcgctg aattttagct ggggcgtatt aacacccacc gccagcgcca
     7021 attttattag ccgcgcctcc agcagcaaag atcccgtcac cggcaccttt gcctatgggc
     7081 ctttaagcga agcaaatttt gtaatgacac cggaggaatt tgattcgagt tattaccaag
     7141 taagcctcgg caccagcgca gtattcccca atggttttag cgcttttatt accgcgcaac
     7201 aaaccctggg gtatgagaat ttttcatccc aacaatactc gctgggtgtg cgtatagagc
     7261 tttaatagga gagaattatt cggccttggc ggcattgcca tggctacaga attttcagcc
     7321 ccaagtagcc tggatggagc gcaagcgaaa tccgggtttt aacctataaa ccgcagttga
     7381 actcgtagcg agggtgccta aggcgccgaa tatgggcgct tttttatcaa aatttggcga
     7441 aagcagtagt catctctacg gtgagtcaaa ttttgataaa aaagcgtgca gattcaagca
     7501 ttaggcagcc gtagtagagt tcaactgcgg tttttaggtt taatgcacaa ccccggattt
     7561 cgcttaagta aatgaggatt ttttcgccaa atttaacgcc gccagagcta gcaaggtagt
     7621 atttcaacaa gctgttaatg cacaactccg gatttcgctt aggctgcata cgatacaact
     7681 atttaatttg tacccattgc cacgctgtta gcgattgcac agataaaccc tgcagcatat
     7741 tcctgtttat tcctccgcat attttattaa aaacccacca aagggtttaa gtgctagtga
     7801 cacagcttgg cccggtttaa taatgcggcg atcaaattga ttgctctgca taccatcgcc
     7861 aatcaactcg cctttttggt tgcccacaaa atccagattt aaatttaagt ctatgctgtt
     7921 gggctggcca ttaatagcgg ccacatacca ttggttgcct ttgcggcgcg cgattaccgc
     7981 atagcggccg ggaaagccgt caacaaaacg ggtttcatcc caactcaccg gcaaatcgcg
     8041 caggatattt ttaacataat gcggcacctt cgccatgcct gcgggtgttt cggcaatatg
     8101 ttgccagccg gataaaaaca acacactcac agccaattca aaccccacgc gggtgcgcct
     8161 atttatgccg ggaatggacg agagaaccgt cggtgtaaaa tccatgggat caaataaatt
     8221 gcgggcgaaa ggcagcatgg ccatatggct gggggctgca tcggcggcgg cttgtgaaaa
     8281 ggtaataaat tccaacccct ttaccgcttc catagttaaa atattcgggt aggtgcgctg
     8341 taaacctcta ggcaaggtgg cgccgtggta attaagcatt aaattaaatt gggctgcgtc
     8401 ttcggctaat tgggtgtaat aggccaacat ggattgccca tcgcctgcaa aaaaatccac
     8461 ctttaggcct ttaatgccaa tgcgctggag ccaggcgaat tcctcgagcc tgcgtgagcg
     8521 gtcgattaat atatttttgg gtgtttgggg tgtggtattc caggcgcccg atgagttata
     8581 ccacagaatt aagcccacac ctttactcgc cgcatactgt gccagctgcg ccatttgcag
     8641 tttgccaatt tggctatccc acagggcgtc aattaataca tagggccaat gcatgcgggc
     8701 ggcataatca ataaatgcgc gctgtgtgtt gtaacgagtg ccttcatctt ttaatagcgc
     8761 ccaactccag gccactacac ccggctggac aaaatccata ttcgccttag ctggtaacgc
     8821 aaaatcagtt cccaaggtgg attccactaa attcttaagg ctgcccatgg cgataatccg
     8881 ccagggcgtt tcaataatag gcgcatctgg cgctaataaa gcgccgccgg taaacacctc
     8941 tggcgccgaa ggttggccaa ttaaatatcg gcctgggctg gtggcatccg tttgtaaacg
     9001 cgacgcgtga aaatgcccat ccatacccgc ttcggtaatc gcaagccagg tgtcgccgct
     9061 gcgaaataag gcaggaaatg cccagccagc ggcagatact ggcgcgccat cggcggcaat
     9121 atccatttga taatattctt cgtaggaagg gttggtattg gcgtaaccgg tttgtgcagg
     9181 ggctatgggc tgcaaccagg ctttagcgtc ggccggcaag gtaaattggc ttaattccgt
     9241 taaaaaatgt tgtggcgccc cgccatgggc cagtgcgcgg tatttaaacc caataccatc
     9301 gttaaatacg cgaaaaataa cctccagcgt attattttct ttattgcgca ggcgcaccac
     9361 acgctcatta gcagcattat taaccgcatg ttttttgcca tgcttaagca gataagactc
     9421 ctgcagcggt tgaatatccg ctatctgcac caggcttaaa ccctccacaa attcattatg
     9481 gtgtagcaca aggccgaggc cggacgacag cacaaccatc tgcccgtggc ggcgaatgct
     9541 ataacttaag cggccctcgc tggataacac caactccacc tgtatgctgc cgtccgggct
     9601 gccaagcgca gtgacgggcg ttgcttgatc ggcgcgagct ttaaattgtg ttgctggtgt
     9661 ttgatgttgt gcacaggcgc acaaacacag caacagaaga ttgaacaaga agaatttttt
     9721 catcggttaa ggccggctgt taaacgtaac aataaaagtc aacgttttat acatgcgcca
     9781 aaatcacgcc gcacaaaaca gcccgcaagt gttacacatt cagcctaaaa ataatgctgc
     9841 agctggcgac tgaacagcaa aaacccgcaa tcaatcacgc cgcccggccc actcagggca
     9901 aacagagaaa aacgcctgcc gcacaggcat gaaattgcgc gctatttata gcgcttaact
     9961 accaacagag cgccttgggc gctaaaaata ctctgatgac tgctgccttg gggcgcaaaa
    10021 tgcacggcgc ctggctcgca gtattcgccg tccaaccaaa tgtcgccgct aattaatatg
    10081 gattcttcat cggtatcgtg gtggtgagca ggcaagcggg tgtcgggcgc caaacgccat
    10141 aaataggtat cgtggcgcgc acaggtatgg gcctgtttaa attcaacccc tggcgccata
    10201 agcgtccatt caccctcgtc taaatggcag gtattaaata ggctattgcg atggtggccg
    10261 cggccaacgc ggcgattaat acgcatttct aaggagctgt cgggattttc agttggctgt
    10321 tgcataaatt cgctccgatt acacaaggga ttgttgcaag cgcgccatgg cgcggcgggc
    10381 atggcttttt accgtgccta aaggaagccc cgtcgcttgg gcagtttctt cgtgggttaa
    10441 ccccaaacgg tacagagcgc ctaatacttg ttgttccggc gggcttaatt gtgcgatggc
    10501 cttttttaac ggcccggcaa ataattgaat attccattcg aagggatctg cctgagcact
    10561 cccctcttcc aaatactcgg gggtatcggc aaataaatcc catttttgcg catggggttg
    10621 ccgccaatat aataatgcgc gggatttaca taacatgctt aaccaagcca ccaaactccc
    10681 cctgtgggcc tgccattgct ccatatgctg ccaggcgtgt acaaatacat cctccacaac
    10741 ctcttctgca gcgcctttat cctgtgtaat ccgcagcgca aggcgcagta atttccccga
    10801 catcaattga tataactgat gcaaggcgcg aggatcgcgc tctttaatgg ctgcagccag
    10861 cgcagcatct gaacaattag cggtatcaag ttcaggcata tttcccccca gtgacatcct
    10921 tcataaaaag gccacccgct cagaagcatt ttgtacacag gcggcctgta tggattttac
    10981 cggcagttaa tgccgaattc ttcgttagca ggcaaattca ccacaaaact attattgttg
    11041 cgcatattga tcgcgtaata accggggccc gggaaattgc ccagatagct atttaacgcc
    11101 acaaaataat ccttggtgta tttatccgtc cccggcacta aattaaagtc ggcgcttaaa
    11161 tccgggcgat ataacagctt aatattcacc cgctgggcga aattaccaaa ccccaccact
    11221 tcgctctgca ccgcattatt aaacgctggt tggcccgggc actctatttt gacccgcgca
    11281 tccggcccga atgaaatggg gccgcgaatg gagctggcgt gtgcgggcac caccaagagc
    11341 atagaagtgg caagtgcggc caagcaatgt gttgagaatt taattgaaag aggcataaaa
    11401 atctccaagt agaggataaa caacgtctgt tgctgcggcc gcattacctt tactatgcat
    11461 tcacggggca ctttggatgc acgctcttat gcgctatttt tctcatacta gtcttcagca
    11521 gaatgcgctg agtattaacc cggcggcttt tgatgccggg ttaataaatc acataaagcc
    11581 ggtatattgg cttattttta gctgcgcctt gccaatgcat tcgttaatca ccccgcagcc
    11641 taaagggata aaaccctgta gtcttttcta gcgggctaaa gtctgcgtat gcatcacaaa
    11701 atttattgta cccaggcgag tttggctggc tctttagttt gagaagtttt aatgtgtcag
    11761 cgttgctgta tcacgcgctg gcaaccccat cacaggcacc ccattctgct catatttaac
    11821 tcaattaagt agcgagcaga cattaacgat taataaaacg attgataaat aggtgcttta
    11881 ttcttccgta caataataga aaggtatttg tatgtttttt acaaacaagc cgttatggac
    11941 gccggtattg ataaccggct ttttaactat gggtttaact gcctgcggct ccgggagcaa
    12001 atcaaatccg ggcccaagct caagcagctc atcctccagc tcaagttctt ccagtagttc
    12061 ctctagcagc tcgtcaagca gctcgtcaag cagctcatcc ggcgccgccc tcgctgcaaa
    12121 tttactcacc agcgacggca gttttgcctc tggcaagatg aattttagcg ataaccctac
    12181 cgatgcaacc ctcacctaca acggcgaatt taaggcagtt atcaataagg tatctactga
    12241 atcttggcat gttcaattaa cccacggcct tgccatcaaa caatccacca cctacaccat
    12301 ttgttttgac gccaaagccg aggctgcgcg ctccattaca tttgacatag acgcagctgg
    12361 cacacctggc tatgacagct taatcggcag ccaaaccgcc gcgttaacca gcgattatca
    12421 aacgattaaa tataccttcg ccgccacctc caccgatgac accgcccgtt tagcctttaa
    12481 tatgggtaag agtgatgtgg atgtttattt ggataatatc ggtgtatttg aaggcaataa
    12541 ttgcggcagt tttgccgccg ccccgctgcc cggcacaccg ccgattgccc ttgccgaaag
    12601 cggtttaatt ggcaatttgg ccaatgggaa aacccaatac gacagcaaat gtgctggctg
    12661 ccacggcgtg gctggcgcag gcgcgtcggc acccgcttta aataccaatg cagtggtgcg
    12721 cacaaaaaat ggtaccgcct atagccttga aggctatgtt gcggcattta tgccgaaatc
    12781 agcgcctggc acttgctccg gccaatgtgc agcggatgta gccgcctacg taaaagcggg
    12841 ttttaatgtt cccagcaaaa ctacctgttc ggcgggaaca ggcgtaatct gcgaggattt
    12901 tgaagaggcg agccttaaca gccgctggac tagtgtaggc accaccgagt taacagttga
    12961 agaaaaaagc tcgggaacta aatcgctaaa aatctctgct ataggtggca aggttaattt
    13021 taacggtgtg gatttagatt taagtaaatt caccggcctg gccaaaaacc attacgggcg
    13081 cgtcatgtta aaaacacccg cagtgagtga aaacgcaggt gattttactt ttatcgaggc
    13141 atcaggccct gcaaatgtat tggccaccac tcgcttagcc gagacgaatc gcgccattta
    13201 tcgcgcccgt gtggacggca accatgaaag ttttatgggc aattacgaca cctcacccta
    13261 cggtgcaccc ctcaccgatt gttggagcca cgccgattat gtagccgtgc ctaaaaatac
    13321 ctgggcgtgt tttgaatggc gttttgatgc caccactaac actctcacct attggctcaa
    13381 tggcactaaa ttgggcatga gcgtgaagga taaggctact ggttcaggtg cggtagccgg
    13441 ttgcgcgggc catccgctgg atgatatttg gtccggccca gccagtttcg ataaattaca
    13501 catcggcatc gaccaatatc actccaccag caaagcgcgt acgctgtata tcgacgatgt
    13561 agtggtggat accgaacggg tcaactgccc gaattaatca ggtgctaagc gcgcaggatg
    13621 gtaattctgt gcgcctaaaa gccgatccag caatgggtcg gcttttttat gggggcagag
    13681 attaatccac acttggtttt cgttatgttt accagaggcc ttttttaaaa gtcttgcacg
    13741 gcctcgccaa cgcaataacc aagcttgtag ctaataattc tagcctcgtt caggtaggcc
    13801 aaaagatttg ccgtatgtcg gccatcaaaa tagtcattat aaaaaatgac accgaccgag
    13861 aggcgaatta aaagattact catttcccat accttctgca ctggatgccc tcaataatca
    13921 gcctttgaag ggccgcacag ctgtacctta tctttgttat cacctaacac gggggctaat
    13981 ttttcccatt ctttatagtt gcggtccaag caaagggtcc ggtttagtag ctgctcctgc
    14041 gacccgccag aaaggcgcca acccagagga ggtttaaccg gatctggttt atcgcgccgc
    14101 tcttcgggga tcaatgcacg aaaatgtaaa taaacaccgt tttggccacg agctaacgtc
    14161 caggcccgta ggcggtcgcg aacatcttcc tggtgtgagt ctcgcgcaac aaagaaaccg
    14221 aagactgggc ttagcaaatc gttggcaacg tgaggggcac gagcaacttg atcagcaata
    14281 aattctggct gcgctgtttt gtagtccggt tcaaacggcc ggggagaatc gatagagatt
    14341 tggatgacca ctacttgcat attatcgagt cggttgccta gagcaattaa agactgcatc
    14401 agtgtataag caccgtcgtt ctcgaaatag ccgccatcaa taagccgccc taatttcagt
    14461 gctggcttgc cgggctctgg ccggatctgc cctgcagggc taaccaacgg aaaacgagca
    14521 ctattgtgag ctgcggtgct gattctcaag tcgtgtctag caacggagaa taaatcaatc
    14581 gcagtctcaa atgggcgccc aacgctcaag tcttcatctg ccaatttgac ttgactttga
    14641 atgactgggc gtcctgtttc ctggtgagtg gaatttaaca ttaaaattgg ttgccacttc
    14701 ccggtaagtg agcgctgctt gagaaaatct tgatcaagac cggagcattc tttttgttct
    14761 gaaaaatgtt cgcgccacat ttcttcaagc ctagtttcga aaactattgc gcgatccatg
    14821 ggtttgactt cgtagtgcgc caagatcgaa ggaccaggtg cccaaaaaaa catatctgta
    14881 aatagcgctg tgccgagaac tggggttaat aaatcttccc ctaacacagc tgcaattttt
    14941 tttcgcatat cggccgaatt catttcacat gtcaaagctt gagtaaatac cgctgcgcca
    15001 actgaaccgc ctgatactga acttatgaga aacaaccgag gcgcaaattc agggtcaatg
    15061 tcttggaagg ttgcaagtag gctcgacgtc caataggctg cagtaatacc tccaccggag
    15121 gcggccacta ctattagtgt tttcgatttt tctccctgca accctttcca tcgagtgaac
    15181 tcatcgatta gtttgggcct tgtgtcaccg ggctgcctat tatcttggtg gagttcgtat
    15241 ggagcactta gtcgaatgta atagggggtg catgagctaa gaattacaga tgtgaatact
    15301 aaaaatgtaa cggtagggaa tgagctttta cgtgtcaaaa aaaccaagta acttccgaca
    15361 caaaccacac cagccaagcc taacattagg gttggcactg tacccaaagt agcgcctaac
    15421 cacaggggtg caatccatgc aaccagggtg ctaacacctg tcataccgag agacagccag
    15481 acaaatactg ggcttgtatc tggatctgcg cttttacgca cgcccatttt cgtgcgcaag
    15541 cgttcgttga gttcacgccg gaatacaatt attaccagga ataaaactga cgacaggata
    15601 agcgcggcca gtacccccca cacatattca ccgccctgac catctattat tgcagcggat
    15661 aaggcaatac ccggaattaa gcaggtaata acggcaagac agcgagggaa tacattggca
    15721 gtaaaatctg gccggtcttc cgaacgaggg tattttgcac tg
//
LOCUS       DAOQGS010000014        15667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1724_length_15667_cov_4.144120, whole genome shotgun sequence.
ACCESSION   DAOQGS010000014 DAOQGS010000000
VERSION     DAOQGS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1724_length_15667_cov_4.144120"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKZ52_01105"
     CDS             <1..603
                     /locus_tag="PKZ52_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58391.1"
                     /translation="VYVDLTSGDRTLISGEARGTGPNFNNCDHLLALDTANNRVFIGN
                     FRALIQVDLSTGNRVVISDPDTNVGSGVDLGSVRALSYDTVNKQVLATVGNGAIVKIN
                     PANGARGIITDANTGTGRTLYSNPGVAIANKNGTHAFIVDTISGFLQVDLLNGNRTLF
                     ADPYKMSTENLSCDFANQRCFSGDIVIDLISGERAAFQLR"
     gene            925..6873
                     /locus_tag="PKZ52_01110"
     CDS             925..6873
                     /locus_tag="PKZ52_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNG58392.1"
                     /translation="MAISIKIRAYFPLILLSLIALTACSKKSNSPPPAASTSSSSSSS
                     GSSKPQDVPAVFSGLSTSPMTIAPGAAEPVTFFTQVSSLAATGTLELEEVDAQGKLLA
                     TLGKLTDSGDLGDARRGDGIFSLQVTLQADQPVIRYFRAKTLVNGVPQVSSIMPLWVT
                     DIPTRTRLPNAGLTVTDPALNLSVYANELVIQLAPGRTPSLAELNALVVPLGGTILGC
                     IPNVARCLVAVATSSAEELAQKATALGQLPGITAAFTNVQTNSPTETIPLSQLPCSGE
                     QCHWYLKSIRAPEAWRISGGGDSARGVAVIDFGVNCALSLLGCANDQPATVEHGTGVA
                     ALVGAKPNQETGTSGVAWNTSLHPLNLLKGDGSQYKMSELIAHSLTQAQAKIINISAG
                     AAADSNNLLRDSLCQAADQGRLSVVAAGNPVPVDGLCESKHIYPARFASESQCSNGAD
                     LKASLLVAGALDQQNRLAQWQAGDYTRCSNLLHATIYAPGQDVLSLNNNNTWSLKTGT
                     SYATPLVSGAAALVWAANPQMTAAEVQTRLLTSAATLSANSADARTKTPDARLEGKRL
                     LDTFKAVNGATPATDSIITPQEFTFAPKSAPAGGEVTSEAVALVGITGPSPISIQGGY
                     YSINNGAFTDIASVILPGQTIRVLTRASNTAGETTQVKVSVGARSQTLSVTATGASVT
                     SPTPTSKPQALRAWDAAADFDPSANPNGVWRYGYNPGAVPGYDFKYFDQYTTELNALR
                     WQDKTYLTAGTPSIVLNTGNPAYYGTPAGQVALHPGPAAQADEAILRFIAPASGLYSV
                     KAQFFGGDFGESNAQVIKNAHLQAALASFPTTSGDPKFAEPRVQLNQGDSLDFVVGNL
                     GDYVGDTTPLSVQIALYELSPPSGSSNSSSTSSSSSSSSSSSSSSSGSSVNSSSSSSN
                     SSSSSSSSTSSSSSSSSSSSSSNSSSSSSSSSSSSSGGPSLLNTAVNLVRDEGGLHWY
                     DAHLFPTDDSEAYIHGALVGMQREEFYKLVGTEFIAEPSYIDGNTLILTANGWTNSKG
                     ILQVTQYNSDGSILLRSLDEGPATFKLAATETLDLSGQTVASFFSNYSDIPGLANRIN
                     PQSQFTAGAKLYKLRPTAINDYYEIWFNPGDESGRCPWDENKLAQNYGGNCETVDTWG
                     PNQSYEGAHTELNELFSADVAPNAEGFRSVWLPGNNLMHIGAQLLNDAAKTVRFYQLD
                     APNGPLLLATGSWSNSKLPYLSSDDGAIILPLPQAALDTGHFEKGVHNLLMVKQAGYI
                     RHGSVTLAGGAFQDSFNVLNGPAHSQVAEALKPSNASACSSTTDSIAHLTPDRTVRSI
                     ALAADGTAYLGGDFTRFAAATGSGVVLNATTGAPRNFPKVDGHVFAVVSDGAGGWYIG
                     GNFGAVGCEPRSNLAHITQDGTLSPWQPTTNGAVRSLVAFENSIAIGGEFNQVNGQSS
                     PYFALLDTSGTAQAITMHPDAPVYALARQENGNLVLGGEFTHIGTQTRPHLASISLMF
                     ISGRVFNLAELTDWSPQTNKAVNALAVAGDSVFVGGTFTEVNGEPVTAFAEITTEGLI
                     GPLTSALSFGGREPVVHALAIANGTVYLGGVFESINGSARNHIAAATLAGQLTDWNPQ
                     LTGNGIFSLATQGNTIYLGGDFSQVNGQTRSLLAAVDNQANLLPWDPNVGGYSVRALA
                     LTDGAVYAGGVFNVLGAGLPRTGLAAVSPTGAITEWNPAVNGVVNSLLLEGEQIYVGG
                     AFDRIGGVSRSNLAAIGTDGVLQSWAPKASGQIATMAIRGDVVYLGGGFEAIDDIPHY
                     FLAGVSKSGVVQDWHAWLNGWVNTLAFDGNTLYVGGYFYEAYGLFRNHVAAFDFTEQS
                     PRLTDWAPDVNGLVNTLTLMDNSIAVGGYFTEVNQQPRLRFAALDKQGALTPLDAKLN
                     GYNVYSLEAARNGIYIGGGFTQASGATRLNSVKLLPDGTLGQWYPGPRFEVHAIQQQE
                     GWVYRGGYGFFEIVRDLSEGAPDT"
     gene            complement(6950..7795)
                     /locus_tag="PKZ52_01115"
     CDS             complement(6950..7795)
                     /locus_tag="PKZ52_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNG58393.1"
                     /translation="MPATAVTVAAVQMVSEPNLAANLKKAAELLSEAAAQGAECAVLP
                     ENFALFGQAGSARQGLLESQGQGEILEFLAEQAKGLNLWLIGGTIPLACNQQGRWANE
                     HKSFASCCVFAPSGEQVSRYDKIHLFDAGVMDAVGSYRESAQFEPGSAPGWFEGPGGK
                     WGLAVCYDLRFPEYFRLLSEAGCHLLAVPSAFVYTTGQVHWEVLLRARAIENQCFVVA
                     SNQGGHHGGSRRTWGHSMIVNPWGDVLAVLPEGEGVISARLDLNELQALRARMPVLSH
                     RRIKT"
     gene            complement(8183..9043)
                     /gene="panC"
                     /locus_tag="PKZ52_01120"
     CDS             complement(8183..9043)
                     /gene="panC"
                     /locus_tag="PKZ52_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNG58394.1"
                     /translation="MHLFHHIKSLRQALQQARAEGKRIGFVPTMGNLHQAHLELVKIA
                     QSQADFVVASIFVNRLQFGLNEDWDRYPRTLDADCEKLRSVQCDVLFCPDEIEMYPNG
                     MDQQAKVILPTMADVLCGASRPGHFTGVTTVVSKLFNIVQPDIAVFGLKDYQQLAIIR
                     RMTEDLCFPVQLVPGPIVREADGLAMSSRNSFISAAERPRVTVLYQSLVQMAAAIEGG
                     ERDFKALEVQARAHIEAAGFRVDYVSICESKSLQPAAHDDTSLQLLGAMYTQGARLID
                     NIALELAAED"
     gene            complement(9121..10083)
                     /locus_tag="PKZ52_01125"
     CDS             complement(9121..10083)
                     /locus_tag="PKZ52_01125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG58395.1"
                     /translation="MASADEFLNEFHWVMDVLQNIDVGLVVLDEEFNVQLWNSFMQNH
                     SARSPSDVLGKKIFELFPELPEAWFRRKVQSVSLLRNSAFTTWEQRPYLFRFKNYRPV
                     TGRAEFMYQNSTIIPLSDVKGKIGHTCIILYDVTEIATNRLQLQRANHQLHIISRTDG
                     LTGLLNRKTWEEILQHEFKRFARYGHTCSLLMFDIDHFKRVNDTYGHPAGDEVIRQTA
                     AVVRNCIRDIDVAGRYGGEEFAVILTDTDAQGALIVAERLRKQVEANTVYYEDHVIHF
                     TISIGIAEVHSRMSEPTLWIDSADRGLYRAKRAGRNQCYIYEPE"
     gene            complement(10086..10697)
                     /locus_tag="PKZ52_01130"
     CDS             complement(10086..10697)
                     /locus_tag="PKZ52_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNG58396.1"
                     /translation="MTRTINLTEEQRDCLQEVTNVAMGQAGDNLARLLDTFVILSIPH
                     IAVMTPSDIAMALQSISSSDSVSGVCQGFIGGGIAGEAILLFNDTSFTDLAKLMNYED
                     VLDEQAERELLMDTTNVLFGACLKGISEQIDMAFSFGPPMVLGQHQRLDTLFDPATAM
                     WRQALVTEINYKLEGYNVNCDLLLIMTEDSIARLLLKLDYLIG"
     gene            10797..11441
                     /locus_tag="PKZ52_01135"
     CDS             10797..11441
                     /locus_tag="PKZ52_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNG58397.1"
                     /translation="MQFRIIPVTPYQQNCSLIWDPATMEGALVDPGGEPERLLAQSQQ
                     LGVNLRSILLTHGHLDHVGATAVLSQQLNIPILGPHPDDQFWLDDLDGQARWMGFKPV
                     ARFEPNRWLHEGDKVRIGSCELEVLHIPGHTPGHLVFVHRPSRLAWVGDVLFAGSIGR
                     SDFPRGDHDLLVRGIAEKLWPLGDDVRFIPGHGPMSSFGQERRSNPFVADKQFG"
     gene            11530..11904
                     /locus_tag="PKZ52_01140"
     CDS             11530..11904
                     /locus_tag="PKZ52_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HNG58398.1"
                     /translation="MTVVDPLGSDLDENYERFLAEAEATGCVWGLEGPDGWALCQSNL
                     NDELSVMPLWSQPEYAQIHCADEWASFKVTPISLEELLDDWLVGMHEDLTLVGPNWNQ
                     DMEGDEIEPLDLLAEFEDVDDS"
     gene            complement(12467..12859)
                     /locus_tag="PKZ52_01145"
     CDS             complement(12467..12859)
                     /locus_tag="PKZ52_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58399.1"
                     /translation="MSLQPAVSLPLVVDNSFASRPSDEVYWLRVLPPETSWRGQHPKI
                     WQLVHEFDICVLERLRQASLAGNVDFVLATRTSCSNGLRVITGHCYLSPAFSDLAQLE
                     LYLGEYLVDLLHMHFFGDPKIEQSIAFG"
     gene            complement(13034..13894)
                     /locus_tag="PKZ52_01150"
     CDS             complement(13034..13894)
                     /locus_tag="PKZ52_01150"
                     /inference="COORDINATES: protein motif:HMM:NF022665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HNG58400.1"
                     /translation="MSDSPSLLARALIPLGVIILLAVAAAIFLFYLQSQSPAVTKPLA
                     HLPAPEAAKAPAPAVPVAQVPPVEVPAAQTQLAPPAPEAPHVPRADAFSDTGTLWGAL
                     QAALAPKIAALLLPDEFVRKFTIAVYGLSEGRVVAQNRPYVSPEGLFAVAKLSDGPQA
                     QFRLLPQNAERYETYLEALRWANSDTGIALYERAYPQLQAAFEELGLGKRSFHQIAII
                     AIDNLLAAPDVTGELILEQPKTRYTYLDAELEKLPATHKLMLRIGAKHNQVVKQQLRQ
                     LRQRLVAIKF"
     gene            complement(13990..15393)
                     /gene="dnaB"
                     /locus_tag="PKZ52_01155"
     CDS             complement(13990..15393)
                     /gene="dnaB"
                     /locus_tag="PKZ52_01155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNG58401.1"
                     /translation="MSQADSSADFYSDAPPSGPQELTLPHSIEAEQAILGGLMLDASG
                     FDGVAEAVGEQDFFRPAHKKVFGIMLHLLEHEQPIDVVTVSEELQRRGELESVGGHSY
                     LIDLAANVAGNANLVAYARIVRERSTLRQLIQAATDISRASFNPGALAADELLQMAEK
                     KVLQIAEERPKEGGFIGVNQLLKTTVEKIDKLFRSESDITGLATGLVELDNKTSGWQP
                     GELIILAARPSMGKTALALNFVEAGIMTQPQPVLVFSMEMPADSLVMRMISSIGRIDQ
                     GRIRNGKLLEDDWPKLEMAARKMKDKLLFIDDTPGLTPAEVRARVRKIAREYGNPGLI
                     MLDYLQLMHVAGSTEGRTQEISEISRSLKALAKEYSCPVIALSQLNRGVESRPNKRPM
                     NSDLRESGAIEQDADVILFIYRDEYYNEESPDKGIAELIIGKQRNGEIGTCRAAFVGK
                     FTRFENLAPEYYQDFRE"
BASE COUNT         3744 a         4461 c         3979 g         3483 t
ORIGIN      
        1 gtgtacgtag acttaaccag cggcgaccgc actctgatct ctggtgaggc acgcggcact
       61 gggcctaact ttaataattg cgatcattta ctggcgttgg atacagcgaa taatcgcgtg
      121 tttatcggca attttagagc attaattcag gttgatttat ctacagggaa tcgtgtggtc
      181 atcagcgacc cggatacaaa tgtgggcagt ggagtcgatt taggcagcgt gcgcgccctg
      241 tcgtacgaca ctgtaaataa gcaggtttta gctaccgtcg ggaatggcgc aattgttaaa
      301 attaatcctg ccaatggagc aagaggtatt attacggatg ccaataccgg cactggccgc
      361 acactttata gtaaccctgg ggttgctatt gccaacaaaa acggcaccca tgcatttatt
      421 gtggacacta tctctggatt tttacaagtg gatctgctca atgggaatag aactctgttt
      481 gcagatccct acaagatgtc gactgaaaat ttatcgtgtg acttcgctaa tcaaagatgt
      541 ttttccgggg atattgtgat tgatttaatc agtggtgaaa gagcggcttt tcagcttagg
      601 taaacacatg ggctaatttg gtttttttgc tttgtttaag cgccaggatt cagttttaca
      661 cccgggcgat ttgaaaactt gagccattgg cagaaccaaa ccaatttagt cattgcgaaa
      721 acactaatcc aacccgtatt tactgcccga tccacaccca ggcccggctt tggtaaaacc
      781 tggtaacagc gcgctaaaaa cgggattttt aatcgggcaa tcgatcgccc tttaaatata
      841 accaagattt ttactcatag tttttcgagt ttaaatttga acaacccatt ccattcatat
      901 tgagttttta caccaggaac caacatggct atcagcataa aaattcgcgc gtatttcccc
      961 ttaatccttc taagtttgat agccctcact gcctgcagta aaaaatccaa ttccccaccg
     1021 ccggcagcat ccacatccag cagcagctct tcctcgggca gcagtaaacc tcaggatgta
     1081 cctgcggtgt tcagcggttt gtctacatcc cccatgacca ttgctccggg ggcggctgag
     1141 ccggtcacct tttttacgca ggtcagctcc ctcgcggcaa caggcaccct agagcttgaa
     1201 gaggtggacg cccagggcaa gctgctggcc acccttggca aactcaccga tagcggggat
     1261 ttaggcgatg cgcgccgggg cgatggtatt tttagcctgc aggtgacact gcaggcggac
     1321 caaccagtga tccgctactt tcgtgcgaaa accctggtaa atggggtgcc gcaggtttcc
     1381 tccattatgc ccctgtgggt caccgacatc cccacccgca cccgcctgcc caatgcgggc
     1441 ctgacggtaa cagacccagc tctgaattta agtgtttatg ccaatgagct ggtcatccag
     1501 ctcgcccctg gccgcacccc tagccttgca gagctgaatg ccctggtagt accgctgggc
     1561 gggacaattc tgggctgtat tcccaacgtc gcccgctgtt tagtggccgt ggcaacttca
     1621 agcgccgagg aactggcaca aaaggctacc gctctgggcc aattgcccgg tatcacagca
     1681 gcctttacta atgtacaaac caactcgccc acggaaacca tcccactctc ccagctaccc
     1741 tgcagcggcg agcagtgcca ctggtatctc aaaagcatcc gcgcccctga ggcctggcgc
     1801 atcagcggtg gcggtgacag tgcgcggggc gtggccgtga ttgattttgg tgttaactgt
     1861 gcgctgagcc ttttaggctg tgcgaatgac cagcctgcga ccgtggaaca cggcaccggt
     1921 gttgccgccc tggtgggcgc taagcccaac caagagacgg gcacatcggg ggtggcttgg
     1981 aataccagct tgcatccact caaccttctt aaaggcgacg gcagccagta caaaatgagc
     2041 gagttgattg cccacagcct gactcaagcg caagccaaaa ttatcaatat cagtgcgggg
     2101 gccgcggcag atagcaacaa cttattgcgc gactctctct gccaggcggc cgatcaaggg
     2161 cgcttaagtg ttgtcgctgc gggcaaccca gtgccggtgg atggcctgtg tgaatccaaa
     2221 cacatctacc cggcccgttt tgcaagcgag agccagtgca gcaatggcgc cgacttaaaa
     2281 gcgagcttgt tagtggccgg cgcactggac cagcaaaacc gcttggcgca gtggcaagcg
     2341 ggagactaca cgcgctgcag taacctgctg cacgccacga tttatgcccc cggccaagat
     2401 gtactcagcc tcaataacaa caatacttgg agccttaaaa ccggcaccag ttacgccacc
     2461 cccttggtat ccggggcggc ggccctggtt tgggcggcca atccacagat gactgcggcc
     2521 gaggtgcaaa cgcgcctgct caccagcgcc gccaccctca gcgccaacag tgccgatgcg
     2581 cgtactaaaa cccccgatgc gcgcctggaa ggcaaacgcc tgctggacac tttcaaagcc
     2641 gtgaacggcg caacaccggc caccgacagc atcatcacgc cgcaagaatt tacttttgcg
     2701 cccaaaagcg cgcccgctgg cggagaagtt accagcgaag ccgtcgcgct ggtcggtatt
     2761 accgggccaa gcccgatcag catccaaggc ggctactact caatcaacaa tggtgcgttt
     2821 accgacatcg ccagcgttat tctgccgggg caaaccatac gcgtgttaac acgggcctcc
     2881 aacaccgcag gggagacgac ccaggtcaaa gtgagtgtgg gcgcccgcag ccagaccctg
     2941 agcgtcaccg ccactggagc gagtgtgacg agcccaacgc ccaccagcaa accccaagcc
     3001 ctgcgcgcgt gggatgccgc cgccgatttt gacccatccg ccaaccccaa tggagtttgg
     3061 cgctatgggt ataacccagg cgctgttccc ggctatgact ttaagtactt tgaccaatac
     3121 accaccgagc taaacgccct gcgctggcag gacaaaacct acctcactgc gggaacgccc
     3181 agcatcgtat taaacaccgg caacccggct tattacggca ccccggcagg ccaggtggct
     3241 ctgcatcctg gcccagcggc gcaggcggac gaggccatct tgcgctttat cgccccagca
     3301 agcggcctct actccgttaa ggcacagttt tttggcggcg atttcggtga gagcaatgcc
     3361 caagtcatca aaaatgcgca ccttcaggca gcgctcgcaa gcttcccaac caccagcggc
     3421 gaccctaaat ttgccgagcc acgtgtgcaa ctgaaccaag gcgatagcct ggattttgtt
     3481 gtgggcaacc tgggcgatta tgtgggtgat accacccccc taagcgtaca aatcgccttg
     3541 tatgagctca gcccgcccag tggttcatcg aactcatcat ccaccagctc gtcctcaagt
     3601 agttcgtctt ccagcagttc atcgtcgagt ggctcctcgg ttaactcctc ctcgagcagc
     3661 tccaactcgt cttcaagcag ttcttcctca actagctcgt cctcgagtag ctcaagcagt
     3721 tcttcctcaa gtaattcaag ttcttcttcc agcagctcat cctcaagctc atcgggcggc
     3781 ccaagcctgc tcaatacggc ggttaattta gtgcgtgatg aaggcggcct gcattggtat
     3841 gacgcccacc tcttccccac cgacgattcc gaagcctata ttcacggggc actagtgggt
     3901 atgcaacggg aggagtttta caagttggta ggcacggaat ttattgccga acccagctac
     3961 atcgatggca ataccctcat cctcaccgcc aatggctgga cgaattccaa gggtattttg
     4021 caggtcactc aatacaacag tgacggctcc atcttgctgc gcagccttga tgaaggacct
     4081 gccacgttca aacttgccgc caccgaaacc ctggatctta gcggccaaac cgtggcgagt
     4141 tttttcagta actattcgga tatacctggg cttgccaacc gcattaaccc acaaagccaa
     4201 tttaccgccg gggccaagct atacaaactg cgccccaccg ccataaatga ctattacgaa
     4261 atctggttta accccggtga tgagagcggg cgctgcccgt gggatgaaaa caaactcgcg
     4321 cagaattacg gcggcaactg tgaaacggtg gatacctggg gccccaacca gtcctatgaa
     4381 ggcgcacata cggaattaaa cgaactgttc tctgccgatg tagctccgaa cgcagaaggt
     4441 ttccgctcgg tgtggctgcc gggtaacaac cttatgcaca taggcgccca gctgctcaat
     4501 gacgcggcta aaaccgtgcg cttttatcag ctcgatgccc ctaatggccc gcttttactg
     4561 gccaccggta gctggtcgaa cagcaaactg ccttacctca gcagtgacga tggggccata
     4621 atcctgccct tgccccaagc tgcgctggat accggccact tcgaaaaagg tgtacacaac
     4681 ttactgatgg ttaaacaggc gggctatata aggcacggtt cagtgacgct cgcaggaggc
     4741 gcgtttcaag acagttttaa cgtcttaaac gggcctgccc acagccaagt tgccgaggca
     4801 ttaaaaccca gcaacgccag cgcctgctcc agtacaaccg actccatcgc acacctaaca
     4861 ccggatcgca ccgtgcgctc cattgccctg gccgccgatg gcactgccta tttaggtggc
     4921 gactttaccc gtttcgccgc cgccaccggc tcaggggttg tactcaacgc caccaccggg
     4981 gcgccccgca actttcccaa ggtggatggc cacgtcttcg ctgtcgtgag cgacggtgct
     5041 ggcggttggt atataggggg gaactttggc gccgtaggct gcgaacccag aagtaacctg
     5101 gcccacatca cccaagacgg caccctgtcg ccctggcaac ccaccaccaa cggcgcggtg
     5161 cgctcactag tggcattcga gaactcaatt gccatcggcg gtgagttcaa tcaggtgaat
     5221 ggacagagct cgccttattt cgccctgctc gacacctcag gcacagcaca agcgatcacg
     5281 atgcacccgg acgccccggt ctacgcactg gcgcggcaag agaacggcaa cctggtgtta
     5341 ggcggcgagt tcacgcacat cggcactcaa acgcgccccc atctggcctc cataagcctg
     5401 atgtttatca gcggccgagt gttcaaccta gccgagctga cagactggag tccacagacg
     5461 aataaagcgg ttaacgcctt agccgtggcg ggcgatagcg tcttcgtcgg tggaaccttt
     5521 acggaggtaa acggcgagcc agtgaccgca ttcgccgaaa tcaccaccga ggggctgatt
     5581 gggccgttga cttccgcgct gagcttcggc ggcagggagc cggtggtgca tgcccttgcc
     5641 atagccaatg gcactgttta tctgggcggc gtgttcgaaa gcattaatgg atccgcccgc
     5701 aaccatatcg ccgccgcgac cctcgcaggc cagctcaccg actggaaccc gcagctgacc
     5761 ggcaacggga tatttagcct ggcaacacag ggcaacacca tttatttggg cggcgacttt
     5821 agccaggtta atggccaaac ccgctccctg ttagccgccg tggataacca agcaaatctg
     5881 ctgccctggg atccgaatgt cggcggctat agcgtccgcg cgctggcact gaccgatggc
     5941 gcggtttacg ctggcggtgt ttttaatgtg ttaggcgccg gcctgcctcg tacagggctt
     6001 gccgccgtat cgcccactgg cgccatcacc gaatggaacc ccgcagtaaa cggagtggta
     6061 aatagcctgc tgctagaggg cgagcagatt tatgtgggcg gagcctttga tcgcatcggg
     6121 ggtgttagcc gcagtaatct agccgccata ggtaccgatg gagttttaca gtcctgggct
     6181 cccaaagcct cgggccaaat tgccactatg gccataaggg gtgacgtggt ttatctgggc
     6241 ggcggcttcg aagccataga cgacataccc cattattttc tcgccggggt ttccaaaagt
     6301 ggcgtcgttc aagactggca tgcctggcta aatggctggg taaacaccct agcatttgat
     6361 ggcaacaccc tgtacgtggg cggctatttt tatgaagcct acggcctctt tcgcaatcat
     6421 gtggccgcct ttgattttac cgagcaatcg ccgcgtttaa ccgactgggc gccggatgtg
     6481 aatgggctgg tcaatacact taccctgatg gacaactcga ttgccgtcgg cggttacttt
     6541 accgaggtca accaacagcc tcgactgcgc tttgccgctc tggacaaaca aggcgcccta
     6601 acccctttag atgccaaact caacggctac aatgtctact cactggaggc ggctcgcaat
     6661 ggtatctata tcggcggggg ctttactcag gccagtgggg ccacgcggct taactcggtt
     6721 aaattgttac ccgacggcac gcttggccag tggtatccag gccccaggtt tgaggtacac
     6781 gccatccagc agcaagaggg ctgggtatat agaggcggct acggtttttt tgaaatcgtc
     6841 agggatctca gcgaaggcgc acccgatacc taaaaaacac cgcgcacagg gatgtgtggc
     6901 gtatgcggat gagtctcatg tacccgcccc gataaacgtc tgcgccttgt taggttttta
     6961 ttcgccggtg gctcaacacc ggcatgcgcg cccgcaacgc ctgtaattca tttaaatcca
     7021 accgcgcgct aatcacgccc tcgccctccg gcaacaccgc cagcacatcg ccccaagggt
     7081 ttacgatcat actgtgcccc caggtacggc ggctgccgcc gtggtggccg ccctggttag
     7141 atgccactac aaaacactgg ttttcaatgg cccgcgcccg cagcaatacc tcccaatgca
     7201 cctggccagt ggtgtacaca aaggccgacg gcaccgctag cagatggcaa cccgcctcgc
     7261 ttaacagacg aaaatattcc gggaagcgca ggtcataaca caccgccaaa ccccacttgc
     7321 caccagggcc ttcaaaccaa cccggcgcgc tgcccggctc aaattgcgcg gactcccgat
     7381 aactgcccac ggcatccatc acccccgcat cgaataaatg aattttgtcg taacggctca
     7441 cctgctcgcc actgggggca aacacacaac aactggcgaa ggatttatgc tcattggccc
     7501 agcggccctg ttggttacag gccaaaggaa tagtgccacc gatcagccac aggtttaacc
     7561 ctttggcttg ctcggctaaa aattccagta tttccccctg cccctggctc tccagtaatc
     7621 cctgcctagc cgaaccggcc tggccaaata acgcaaaatt ctccggcaac acggcgcact
     7681 ctgcgccctg ggcagcagct tcggataata attcagcggc ttttttaaga ttagcggcaa
     7741 ggttgggttc actcaccatt tgcacggcgg cgacagttac ggcggtggca ggcatgggta
     7801 cctcttaaac ctcaagttca cttaataact atagatgccg ttttttacag cgggacagat
     7861 cgcttaacgg caatttaatg cacgcggtca cctacgggat taagtgcatg ctgcaattta
     7921 ttgagaaaac atatactgtc actgccagct aagaaaactt taaaggcggt aatcagtatc
     7981 agggtgctta ttaacccggc ggctaaatag gatcgtcctg atctatttag ccttcgctgc
     8041 cctaaaactg gcgaaaatgc gtggattttc ttgttttagg gcagctcacg gcggtttgca
     8101 ccgccggtgc ggcatccacg ccgccattat taacccggca caaaatgccg ctgggttaat
     8161 atttaaagac gcccgactca gatcaatcct ctgcggctaa ttccagcgcg atattgtcaa
     8221 ttaaccgcgc cccttgcgta tacatagcgc cgagtaattg caaagaagta tcgtcatggg
     8281 cggccggttg caggctttta ctttcacaaa tactcacata atccactcgg aatcccgccg
     8341 cttcaatgtg cgcgcgcgcc tgcacttcca gcgctttaaa atctcgctcg ccgccttcaa
     8401 tcgccgccgc catctgcacc aggctttgat ataaaaccgt tacccgcggg cgctcggcgg
     8461 cggaaataaa actattgcgc gagctcatgg ctaaaccatc cgcctcgcgc acaatcggcc
     8521 ctggcactaa ctgtactggg aaacacaaat cctccgtcat acggcgaata atcgccaact
     8581 gctgataatc ctttaaacca aagacagcaa tatccggctg cacaatatta aataatttag
     8641 acaccaccgt cgtcacaccg gtgaaatgcc caggccggct cgcaccgcac aaaacatcgg
     8701 ccatggtggg caaaatcacc ttggcctgct gatccatgcc gttgggatac atttcaattt
     8761 catcggggca aaataaaaca tcgcactgca ctgaacgcag tttttcgcag tcggcatcca
     8821 gagtgcgcgg gtagcggtcc cagtcttcgt tgaggccaaa ttgcagacgg ttgacgaaaa
     8881 tactcgccac cacaaaatcc gcttgggact gggcgatttt gactaattcc aagtgggcct
     8941 gatgtaaatt gcccatggtg ggcacaaagc caatgcgctt gccctcggca cgggcctgct
     9001 gcagagcttg acggagggat ttaatgtgat ggaaaagatg cataggctgg cccgcagtta
     9061 aaatcgggcc gcatcttaac agcttgccgc ctaatgctta agccgtggcg gaattggcgc
     9121 ctattcaggc tcgtagatat aacactggtt gcgcccggcc cgtttggcgc ggtacaggcc
     9181 tctatcagca ctgtcgatcc acaaagtggg ctccgacata cggctgtgca cttcggcaat
     9241 acctatgcta atggtgaaat gaatgacgtg gtcttcgtaa tacaccgtat tggcttccac
     9301 ctgtttgcgc agccgctcgg ccacaattaa tgcgccctgg gcgtcggtat cggtgagaat
     9361 tacagcgaat tcctcgccgc cataacggcc cgccacgtct atatcgcgaa tacaattgcg
     9421 caccacggcc gctgtctgcc gaataacttc atcccctgcc gggtggccgt aggtatcgtt
     9481 gacccgttta aaatggtcga tgtcaaacat caataaactg caggtgtggc cgtagcgtgc
     9541 aaagcgctta aattcatgct ggagaatttc ttcccaggtt ttgcggttga gcaacccggt
     9601 gagcccatcg gtgcggctga taatatgcaa ttgatggttg gcccgctgca actgcaaacg
     9661 gttggtggca atttcggtta cgtcgtagag gataatacag gtatggccaa tttttccctt
     9721 tacatccgac aagggaataa tcgtactgtt ttgatacata aattccgcac ggccagtcac
     9781 cgggcgataa tttttaaaac ggaataaata cgggcgctgc tcccaggtgg taaacgccga
     9841 attgcgcagc agggataccg actgaacttt acggcgaaac caggcttcag gtaattcagg
     9901 gaacaactca aatatttttt tgcccaacac atcgctgggg gagcgcgcgc tgtggttttg
     9961 cataaagcta ttccacagct gcacattaaa ttcttcatcc aagaccacca ggcccacatc
    10021 gatgttttgc aaaacatcca tgacccagtg aaattcattt aaaaactcgt ctgcacttgc
    10081 cataactaac cgattaaata gtccaatttg agcaacaagc gtgcaattga gtcttccgtc
    10141 atgatcaaca ataaatcaca attgacgtta tagccctcta acttatagtt aatttccgtg
    10201 acaagcgcct ggcgccacat ggcggtggcc gggtcaaata gggtatccag ccgctgatgt
    10261 tgccctaata ccatgggcgg cccgaagcta aaggccatat caatctgctc ggaaatccct
    10321 tttaaacagg caccaaataa tacattggtg gtgtccatca acagctcacg ttcggcctgc
    10381 tcatccaata catcctcata attcatgagt ttggcgaggt cagtaaaact tgtgtcgtta
    10441 aacagtaaaa tcgcctcacc cgctatgccg ccgccaataa acccctggca gacaccggat
    10501 accgaatcag agctactaat agactgcagc gccatagcaa tatcggaagg agtcatcacc
    10561 gcaatatggg ggatggagag aatgacaaac gtatccagta gcctggccaa attatcgccc
    10621 gcctgcccca tcgccacatt ggtgacttcc tgcaggcaat cgcgctgttc ttcggtgagg
    10681 ttaatcgtac gggtcatgcc attcatggcc tgggttagga gctgtaatat ctattctagt
    10741 cggcgctagt tagaatgctg gcaaaattca caagaagtta tgcacacggc tttgttatgc
    10801 aatttcgaat tatccccgtt actccttatc agcaaaactg ctcacttatt tgggatcctg
    10861 ccactatgga aggcgcctta gtggacccgg gcggcgaacc tgaacgccta ttggcccaaa
    10921 gccagcaatt aggcgttaac ctgcgcagta ttttgctcac ccacggccac ctggatcatg
    10981 tgggagcgac agcggttttg agccaacaac taaatatccc gatcctgggg ccgcaccccg
    11041 acgatcaatt ttggctggat gatttagatg gccaggcgcg ctggatgggg ttcaaacccg
    11101 tggcccgctt tgagcctaac cgctggctgc atgaaggtga taaggtgcgc atcggcagtt
    11161 gcgagctgga ggtgttgcat ataccggggc acacgccggg ccacctggtg tttgttcacc
    11221 ggcccagtcg attagcctgg gtgggcgatg tattgtttgc cggctccatc ggccgcagcg
    11281 actttccccg cggcgatcat gacttactgg tgcgcggtat cgcagaaaaa ctctggccat
    11341 tgggggatga tgtgcgcttt atccccggtc acggccccat gtccagcttc gggcaggaac
    11401 ggcgcagcaa cccatttgtg gccgataaac aatttggcta aagcctcgca tagttcggcg
    11461 gcgttttagc caggaaagtt gtggtaataa ggttttattt cagtggttcc ccaacacgag
    11521 ggcaaggcta tgactgtggt cgatccatta ggtagcgatt tagacgaaaa ctacgaacgc
    11581 tttttggctg aggctgaagc caccgggtgc gtctgggggc tggaaggccc ggacggttgg
    11641 gccctgtgtc agtccaacct gaatgacgaa ctgagcgtaa tgcccctctg gtcgcaaccg
    11701 gaatacgccc aaatccactg cgccgacgag tgggcgagtt ttaaagtaac ccccattagc
    11761 ctggaagagc tattagacga ctggctagtg ggcatgcacg aggatctcac cctggtgggc
    11821 cccaattgga atcaagacat ggaaggcgat gaaattgagc ctttggattt attggctgaa
    11881 tttgaagacg tggacgacag ctaaccaggt ttttagtgtt ggcttggcgc aacacaatca
    11941 ctcataacaa acgggagccc gcatgaaggc gccgctggct tgaatattgt gtttaatgac
    12001 gtcgatattt gcggtggcat ttgtgtgccg caggtggacg ataaaattta acgccgtaca
    12061 acttgctcag gttaacctaa aaaccgcagc tgaacactac ttgggttttt gggtttaaac
    12121 ttttcaactt cagcaaggaa cccgcctacg tcacccgccc gacgaacaat ctgaataatt
    12181 tatttcaact accacctatt ttcacctatt tttatcggcg cctgcatact gtcgatcagc
    12241 ctaaatcaca gcaagaacac gctgattttt aatgcttatg gatttgtgct ttctcggttt
    12301 ttgcacacat ttgcgcacat aactgtcaat caagtgtgaa taggattggt agtttttgcg
    12361 gcgggattat ttttcttaat tcgcatctgg cttgaactct tagccgccct ataaaacact
    12421 cccgcgccca cctgcaataa aacgacacaa cgcgtgcgac acataatcaa ccaaaggcaa
    12481 tgctttgctc gattttagga tcgccaaaaa aatgcatgtg cagtaaatcc accaaatatt
    12541 caccgaggta caattctaac tgcgccaaat cggaaaaagc gggcgagaga taacaatggc
    12601 cggtaatcac ccgcaacccg tttgagcaac tggtgcgcgt cgccaacaca aaatccacat
    12661 tgcccgcaag gctggcctgg cgcaaacgct ctaatacaca aatatcaaat tcatgtacca
    12721 gctgccaaat tttcgggtgc tggccgcgcc agctggtttc aggtggcagg acgcgtagcc
    12781 aataaacctc atcgctgggg cggctcgcaa acgaattatc aacgacaagc ggcagcgaaa
    12841 ccgcaggctg caatgacatc acgggacacc ttcatttatt tatggttatt ggtgtgaccc
    12901 cattaaacgc ccatacccac ttgtttggcg tgaccacagt cacagccgcg cacgccattt
    12961 gcgtttgctc tatcacattt ttttacattt aaaaaatgcg ccagaatatc cctgagagcc
    13021 tgcaacaaag ctattaaaac ttaatcgcca ccaaacgctg gcgcagttga cgcagctgct
    13081 gcttaaccac ctgattgtgt ttggcgccaa tgcgcagcat taatttatgg gttgccggta
    13141 gtttttccag ttccgcatcc aaataggtgt agcgcgtctt cggctgctcc aaaatcagct
    13201 caccggtcac atccggggcg gcgagcaaat tgtcgatagc aataatggca atttgatgga
    13261 atgagcgctt gcccaacccc agctcttcaa acgccgcttg cagctgtgga taggcccgct
    13321 cgtagagcgc aatccctgta tcggagttgg cccagcgcag ggcctctaaa taggtttcgt
    13381 aacgctcggc attctgcggt aagaggcgaa actgcgcttg ggggccatca ctgagtttgg
    13441 caacggcaaa taacccctcg ggtgacacat aggggcgatt ttgcgctact acccggcctt
    13501 cactgaggcc atataccgcg atggtaaatt tgcggacaaa ttcatcgggc aataataaag
    13561 cggcaatttt aggcgcaagc gcggcctgca gagcgcccca caacgtgcca gtatcgctaa
    13621 aagcgtcagc gcgcggcaca tgtggcgctt caggtgctgg cggcgccagc tgggtctggg
    13681 cggcgggaac ctctacgggc ggcacctggg caacaggcac cgccggggcc ggtgctttgg
    13741 cggcctcagg ggccggtaaa tgggccaagg gtttagtgac cgcagggctt tgggactgca
    13801 aataaaataa aaaaattgcc gcagcgacgg ccaataaaat aatcacccct aagggaatca
    13861 gggcccgcgc cagcagagaa ggagaatccg acataattgc ctcttaagga ttaatgcatc
    13921 tgatcataaa tgctgaaccg ccattgaatc gattgggcgc cacaagttcc cgcgctagtg
    13981 ccttgccatt tactcgcgaa aatcctggta atactccggc gccaaattct caaagcgggt
    14041 aaatttaccc acaaacgctg cccggcaagt gccaatttca ccgttgcgct gtttaccgat
    14101 aattaattcg gcgatgcctt tatcggggct ttcctcgttg taatactcat cgcgataaat
    14161 aaataaaatc acatccgcgt cctgctcgat cgctccggat tcgcgcaggt ctgagttcat
    14221 ggggcgttta ttagggcgcg attccacgcc gcgatttaac tgcgacaacg caatcaccgg
    14281 gcaggaatat tctttagcca gcgcttttaa tgagcgggaa atttccgaaa tttcttgggt
    14341 gcgcccttcc gtgctgcccg ccacatgcat taactgcaaa taatcgagca taattaaacc
    14401 tggattgcca tattcgcgcg caattttgcg cacccgcgcg cgcacctcgg cgggggtaag
    14461 gcctggcgta tcgtcaataa acaataactt gtccttcatt ttgcgcgccg ccatttccaa
    14521 tttcggccaa tcatcttcca ataatttacc gttgcgtatg cgcccctgat cgatgcggcc
    14581 aatagaggaa atcatccgca ttaccagaga gtcggcgggc atttccatgc taaataccaa
    14641 caccggttgc ggctgggtca tgatgcccgc ctcaacaaaa ttaagcgcca aggcggtttt
    14701 ccccatggag ggccgcgccg ctaaaataat taattcacct ggctgccaac cggaggtttt
    14761 attatctaat tccaccaaac ccgtggcgag gccggtaata tctgattcag agcgaaataa
    14821 tttatcgatt ttttcaacgg tagttttaag tagctggtta acgccgataa aaccgccctc
    14881 tttggggcgc tcctcggcaa tttgcagcac ctttttttcc gccatttgca gcagttcatc
    14941 ggcggccaaa gcgccgggat taaaactcgc ccggctaata tcggtggccg cttgaattaa
    15001 ctgccgcagg gtcgagcgct cgcgcacaat gcgcgcatag gccaccaggt tggcattgcc
    15061 cgccacgttc gcggccagat caattaaata gctgtgcccg cccaccgact ccaactcgcc
    15121 ccggcgctgc aactcttcgg acacggtcac cacgtcgata ggctgctcgt gctccagcag
    15181 atgtagcatg ataccgaaca cttttttatg ggcgggacgg aaaaaatcct gttcccccac
    15241 agcctcggcc accccatcaa agccgctggc gtccagcatc aaaccgccca aaatggcctg
    15301 ctccgcttca atggaatggg gcagggtcag ctcttggggg ccgctcggcg gtgcatcaga
    15361 ataaaaatcg gcggatgagt ctgcttgaga cataacaacc tcaatgacaa tggagaacaa
    15421 agggtgagat tagaaatttg ccaaggcttt tggcaagcgc atcctggccg ccacaggggc
    15481 gccccataaa accgagtggc cagcgcataa aaccagcaca taaaaccaac acataaaaaa
    15541 aggcacgcgg gtcgccctgc gtgcctttag tgtatcgaaa gcaaaaactt attccgcttc
    15601 gattaccaat ttaactgctt gcataacgtc tacgtgcaac tgcacgtcga tgctgaactc
    15661 gccgatt
//
LOCUS       DAOQGS010000015        15558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1742_length_15558_cov_5.535187, whole genome shotgun sequence.
ACCESSION   DAOQGS010000015 DAOQGS010000000
VERSION     DAOQGS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15558
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_15558_cov_5.535187"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..692)
                     /locus_tag="PKZ52_01160"
     CDS             complement(297..692)
                     /locus_tag="PKZ52_01160"
                     /inference="COORDINATES: protein motif:HMM:NF021478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2195 family protein"
                     /protein_id="HNG58402.1"
                     /translation="MAILKFNCALALLLIASACTASPPATPKPGTIKIDNALDKCQQI
                     TGQAVDIASIPTLKLDFKTLASTAECGCKSMLNSYSVAIKLDGYSRRVLSGSFKFPNQ
                     SPLTLPLAAQPQLIKGQSLQITLGCEAPL"
     gene            complement(980..2839)
                     /locus_tag="PKZ52_01165"
     CDS             complement(980..2839)
                     /locus_tag="PKZ52_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNG58403.1"
                     /translation="MTDIIDIKSVDDEIYTCLDLDKPKSFFLFAGAGSGKTRSLVEVL
                     KRFRENNVHRLRTNAQQVAIITYTNAACDEIKRRLEFDPAFSVSTIHSFVWELIKPYT
                     NDIKLLLKVRLQDDINELEMAQRKGRAGSKAAEDRPRQIASKRKRLESLNTIRKFTYN
                     PNGENSGRDSLNHSEVIALTAELLSSRPLLQKILIRKFPVLLVDESQDTKKELIEALF
                     TVQRAHKTQFSLGLFGDIMQRIYTDGKADLGLAPLPDDWLTPKKIYNYRCPRRVVKLI
                     NKIRMDVDKNQQEPIKENEGFVRLFIADTSQPIDKAAFEYTVCQKMSDFTNDNLWADP
                     KENKTLTLEHHMAAQRGGFSNFFNALYGVDKLNTGLLDGTLSGITFFSEKILPLISSL
                     EVNNQFLVSRILNKYSPLMFKEQFEHSEKPKTELKKVKDAVGALYTLWENNSNPTLKN
                     ILKEISRSGLFKVPDSLATIADRQEKDFELAEDDLDPIIDAWDKALASSFNEFKSYVQ
                     YISDKSHFGTHQGIKGLEFPRVMVILDDDEARGFLFSYGKLFGAKTPTKADIDNQANG
                     KETSIDRTRRLFYVTCSRAEESLAIIAYTSEPNKIKNNVLAQGWFTEEEITTL"
     gene            complement(2872..5100)
                     /locus_tag="PKZ52_01170"
     CDS             complement(2872..5100)
                     /locus_tag="PKZ52_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG58404.1"
                     /translation="MKICHIDIQNFRKLKICRVELAGESTIFVGANNSGKTSAMDALI
                     MFLKKSKRRELSTTDITLSNWAAINKIASDWVTNASIEPPDLSLNAWRPLLPAIDVWL
                     NVDEFQIHCISHILPTLDWDGGLLGVRLILEPEDPETLYKSYMAAYSAAQETLTARKG
                     TKENKNNESLSTLTLWPTSMREFLDRKLQVFFSVNAYVLDPAHATSDPPQLLTALMEP
                     LESDPFDGLFKINIINAQRGFSDPNSGEGTANTDRRLSSQLRDYFNEHLDPSELPEAS
                     DLDALEAMEAARTAFDQKLKSSFSVAIGELEGLNYPGFSDPQIVLSSKVNPVEGLNHD
                     SAVQFKVVRSEDPSVNDLCLPEKYNGLGYQNLISMIFNLIRFRDEWMRVGKVGKREKI
                     GNTPIEPLHLMLIEEPEAHLHAQVQQVFIKKAYGVLRNQPKLGEKPQFSSQMVVSTHS
                     SHIAHETDFTGLRYFRRKLLASELEIPCAIVVSMSNIFGEGNDTSKFASRYLKTTHCD
                     LFFADAAILVEGPVERMLVPHFIRNHYPELERSYISMLEIGGSHAHRLRPLIEALGIP
                     TLVITDLDSIEKESTGKARPKKGQSLRTGNDTLKCWAPKKTTLDDVLSATPKEKTTAE
                     GLVRVAYPCLTKIQYLSGKNEEAIPYTFEDALVLENLTMFKAITDRKGLLKKMADAVT
                     KATLDEACEEMFIALGKNAKKAEMALELLYTTEPNLLKPPAYIKEGLDWLNTMLIRDA
                     IL"
     gene            complement(6165..6590)
                     /locus_tag="PKZ52_01175"
     CDS             complement(6165..6590)
                     /locus_tag="PKZ52_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNG58405.1"
                     /translation="MHPPCKCHPWPANDGNQFDTESLGKNPNAIQFNAMVYTGARPPT
                     SKPVSPTQEPSLQQLGFDSSQDFHVYTIEWTPKEATFLIDDVVYHRWNSRINLIGLPQ
                     NVLLTIWASNSPSWAGPVTSETVNAKAVYDWVEVWRYTP"
     gene            complement(6987..7844)
                     /locus_tag="PKZ52_01180"
     CDS             complement(6987..7844)
                     /locus_tag="PKZ52_01180"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNG58406.1"
                     /translation="MTKTIGNLAASIRQKLLNFSKTHHEPFQQVLVNFALERLLYRLS
                     ISNYSDRFVLKGALLFRLWFDLPQRPTRDIDFLGFGEADPEQIKTIFAQLIEIDAEDG
                     LIFSTESITAEPIRKAAGYPGIRVQLQASLDNARIPIQCDIGFGDAITSSAVSSEFPT
                     LLDLPAPVLRAYPLETVVAEKLEALIKLARFNTRLKDYFDLWVLSTAEQIDHAVLPKA
                     IATTFKRRQTSLPTTIPVGLTKSYATEKAQQWNAFLKRSKLEAPPLDEVVKELAAIYW
                     PLLEKSNHH"
     gene            complement(7841..8638)
                     /locus_tag="PKZ52_01185"
     CDS             complement(7841..8638)
                     /locus_tag="PKZ52_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58407.1"
                     /translation="MNAVIACCLQRENLFGLLLKTGGRSGGQEGSLPTKTTINNRFIL
                     LSTHMRVPYVYSINNIGLSHMPPQPHAILEKGKVHTTGDLISRLGLSHAQAFRVLQRW
                     QQEGFVVKVGRGLYQLAEDNQQQNPWLAAQHLVPNGVICLASALAFHELGTQSPPAVW
                     VAVASNAWRKQSDYPPLEWVHMGAEALNSGVEVHAMEGGNLRVFSIEKTLADCFKFRN
                     RVGLDVALEALKDAWASNRLNLSKLDAMAKICRVQNVLKPYVEALIV"
     gene            8706..9755
                     /locus_tag="PKZ52_01190"
     CDS             8706..9755
                     /locus_tag="PKZ52_01190"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNG58408.1"
                     /translation="MNSPSESDVLTQALLALKGETGMDFSVSGQVPSSSEADYLIQTP
                     SQSLAVEVKRNIRPAHLGAIIRRIKTLEPTGLLVADYLNPNIAKVLKENDVQYMDACG
                     NSYLNLPPIYVHISGQKPVLNTKQETNKTFDLSGLKVIYGFLCNRQLVDASYREIAEQ
                     TGIALGAVGKVLKSFVGAGFLIDQGKGKKRQLINRRKLLDRWVEAYPEKLKPKLFIGE
                     FIGDNGNWWKNIEIEKYDAYWSGEVAAAKYTDYLKPHVATLYVPEKSGSKLFAAARLR
                     KANNTTNTIDRLVHVYRPFWPERQNHEHTNYIRANTVHPILIYADLIASADTRNLETA
                     KRLYDSAIAEYIAED"
     gene            9718..10563
                     /locus_tag="PKZ52_01195"
     CDS             9718..10563
                     /locus_tag="PKZ52_01195"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNG58409.1"
                     /translation="MTAPSLNISQKIEPSVADLCAIVAGCAMRLQIPYLVVGASARDM
                     VLHYGYAAKVQRATADIDFALQVPSWAAFAALRRELIRNNFSETIATHRLIGPNNWPI
                     DIVPFGGVADERANIQWPPSGDFVMSVLGFVEALNHANIVRLSDMPILDVPVASPEGM
                     TMLKFIAWTDRAADARKKDAKDIAYLLTTYENIPAVSDQLYRELTLMELYSWDITLAS
                     AHQLGINVKQMALPQTRSVISDVLNKQSAKLSVEKLINEMCENPDQYARNAMLIDAFG
                     AGFFS"
     gene            10975..11115
                     /locus_tag="PKZ52_01200"
     CDS             10975..11115
                     /locus_tag="PKZ52_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58410.1"
                     /translation="MPERSARRLLSQLKSEGLLSETSSKSPLRWEIPEHAEPWYFPNL
                     TL"
     gene            complement(11223..12185)
                     /locus_tag="PKZ52_01205"
     CDS             complement(11223..12185)
                     /locus_tag="PKZ52_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNG58411.1"
                     /translation="MRKPHLIQILLAAAQTLLITSLVSCGGNTSSSSSSSSSSSSSSS
                     STSSSSTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGSKFTLLFRDDFNSFDSS
                     RWQLMTHSWAGNLALFSKDTVAVSNGMMTIKLAPAPSGTVVDGETKSFLGAEVRSTAT
                     INYGRVSARAKFAKGSAVVSALVSIYTPWPADDWNEFDMEMLGKNPNAIQFNAMVYTG
                     ARPPTSKPVSPTQEPSLQQLGFDSSQDFHVYTIEWTPKEATFLIDDVVYHRWNSRINL
                     IGLPQNVLLTIWASNSTSWAGPVTSETVNAKAVYDWVEVWRYTP"
     gene            complement(12389..13558)
                     /locus_tag="PKZ52_01210"
     CDS             complement(12389..13558)
                     /locus_tag="PKZ52_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNG58412.1"
                     /translation="MNAVLFNIHDVALLMRVGECSLLAVLFLAHRGAKPYCNLLLALF
                     LLLNAMIALHLLTLWGEAFRFLAFDFSPNVFFIFNFAYFLEGPILYWYIRSVLYKDFK
                     FAPTDILHLIPAAATPIILYFLYYCRPHEIKQGIALNFKDYGATEPLFDWIVHPEKIV
                     IVLYGIACLYQLFKYRKLLKHNYSDIERIDLTWLMLLIGGFMASWLCFLAAHIFGAFA
                     LAPMGEGLAVSANYVLFVLINILIFYSLIYSDLFEGVNEGKNENESAGENTSRDEITP
                     EQLAIIQNAMEKDKLYLNSRLTLEEFSKQTGLHPRLVSSVINRKLEQNFHEFVNRYRV
                     EEVKRVLCAPDSHALAITDIATNAGFNSKAAFNRFFKKFTDMTPTQFREQHQKPL"
     gene            complement(14138..>15558)
                     /locus_tag="PKZ52_01215"
     CDS             complement(14138..>15558)
                     /locus_tag="PKZ52_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HNG58413.1"
                     /translation="VMREAGYIDFLGREDGQVKVRGFRIELGEIEAVLQRHPEVQGAV
                     AKVIGTSPQDAYIAAYVVLAADHPPTIEDLTRYLAGFVPSYMLPSRWVPMDQLPLSAN
                     GKINRAALPNPEPVSHSRSQAAPASPMECLIARHWCEILGLAEVNLGDHFFELGGNSF
                     AAVRLVMALGEELGRDIPITQLVQAPILKDLAALLQAEHQEARAFSHLVDLLPRNPNC
                     PNAFWFHPSGGNVLCYQDLATALAGRFNLVGVQAAKGAKQAMFTSFNAMVASYLAAIR
                     TRQPQGPYYLGGWSMGGVIAYACAQHLIQVGEQVAALWLLDSPAPVERPVPDEQGLLT
                     WFLSDLLQQDTLPALPTLNCANPQLEAVLREIQQQGLLPRGNLAGLKDIYACFCANIR
                     LLHSYTASTLAACVPSFVAMAEQQVETRVAAQSATIWRHLIPTGRTEQLLACNHYGMV
                     SAPIIAKLQAQLCVAQDDVVSA"
BASE COUNT         4088 a         3611 c         3666 g         4193 t
ORIGIN      
        1 ttctgcaata agggtgggaa gttgttgttt aaacggcctc cctcatcttc gctgatgatt
       61 cttacagtaa tatcagctac ttctaaatat tacaccgcgc caaataaaat actcgcttga
      121 taaattgatg aaattgccct caaaagatcc cagaggaatt aacctgaaga acgcagttga
      181 acccgtagcg agagcgtcta aggcttagaa tatgggcgtt tttgtcaaaa aattgggtga
      241 aagcagtagc gcctctacgg tgagaccaat tttttgagaa aaacgttcag atccaattac
      301 agcggcgcct cacagccaag ggtaatctgc aggctttggc cttttattag ttgtggctgg
      361 gcggccaggg ggagggtgag gggcgattgg tttgggaact taaaactgcc ggataaaacc
      421 ctacgtgaat agccgtctaa cttaatagcc acgctgtagc tattgagcat agatttacag
      481 ccacactcgg ccgtgctggc aagggtttta aaatcgagtt ttaaagttgg tatagatgct
      541 atgtctacgg cttgcccggt aatctgttgg catttatcca aagcattatc aattttaatt
      601 gtgccgggct tgggggtcgc cggaggagac gcggtgcagg cgctggcaat taacaacaaa
      661 gctaatgcac aattaaattt tagtatcgcc ataggtgctg cctcctttgg aatagattac
      721 ttgcaaaata agctcagttt tcttcgtggt tttttgatat tcgcgcaaat gggtaaaaat
      781 aacgccttct acttcaatac aaccatggct ataccccctt gtggaatcgt gcagataaaa
      841 tccactgcgg ctaacggggg cgcagtgttt tttgttgtat tatccgccgc ctctaaccgc
      901 gcccggtttt tgccccaatt ggcccaatag ggctcgcaga ggcctgcgtt tgcgccgcgc
      961 ggaatcgttt gatgtatttt tacaatgtag taatttcttc ttcggtaaac catccttgcg
     1021 ctagaacatt attttttatc ttattaggct cactggtata ggcaataata gccagacttt
     1081 cttctgcccg actgcaagtt acataaaata aacgtctagt gcggtcaata ctggtctcct
     1141 tgccatttgc ctgattatca atgtctgctt ttgtaggggt tttagcaccg aataactttc
     1201 cataactaaa taaaaaaccg cgagcctcat catcatcgag aatcaccatg acacgaggaa
     1261 attcgagccc cttaataccc tgatgcgtgc caaaatgcga tttatcggag atatattgca
     1321 cataactttt gaattcatta aagcttgatg ctagtgcctt atcccaagca tcaataatgg
     1381 gatcaagatc atcttcggct agttcgaagt ctttttcctg cctgtccgca atagtggcta
     1441 gactgtcggg cactttaaat aggccgctac ggctaatttc tttgaggata ttttttaacg
     1501 ttgggttcga attgttttcc catagggtat acagtgcacc cactgcatcc tttacttttt
     1561 ttaattctgt tttaggtttt tcgctatgtt caaattgctc tttgaacatt aacggagaat
     1621 acttgttaag tattcgggat accaaaaatt gattattaac ttccaaacta ctaataagag
     1681 gcagtatctt ttcagaaaaa aaagttatgc cagataaagt cccatccaat aagccagtat
     1741 ttagtttatc gacgccatat agggcattga aaaagtttga aaacccacca cgttgtgctg
     1801 ccatatgatg ctctaaggtt agagttttat tttcttttgg atctgcccat agattgtcat
     1861 tcgtgaaatc agacattttt tggcaaacag tatattcaaa ggcggcttta tcgatgggtt
     1921 gactcgtatc cgcaataaat aaccttacaa aaccttcatt ttctttaatg ggctcttgct
     1981 gatttttatc aacatccatt cggattttat tgataagctt aacgactcga cgcgggcatc
     2041 tataattgta aattttcttc ggtgtcaacc agtcatcagg taatggagct aaccccagat
     2101 ccgctttgcc atccgtgtaa atgcgttgca tgatatcgcc aaaaagtcca agagaaaatt
     2161 gagttttatg tgcgcgttga actgtaaaaa gtgcttcgat taattctttt tttgtatcct
     2221 gactttcatc aaccaataag actggaaatt tacgtattaa gattttttgc agaagtggcc
     2281 gacttgagag caattctgcc gttaacgcta ttacctcact atgattaaga gaatctctcc
     2341 ccgagttttc tccattgggg ttgtaggtga atttccgaat ggtgtttagt gactccagcc
     2401 gttttcgttt agatgctatt tgtctcggtc tatcctcagc cgcctttgac cccgctcgcc
     2461 ctttgcgttg tgccatttct agctcattaa tatcatcctg aagccgtacc ttgagtaaca
     2521 atttaatgtc atttgtatat ggcttaataa gctcccaaac aaagctatga atggttgata
     2581 cgctgaatgc aggatcaaat tccagccgtc gtttaatctc atcacatgca gcattggtat
     2641 aagtgataat tgccacttgt tgtgcattgg tccgtaagcg atgaacatta ttttcacgaa
     2701 accgttttaa tacttcgacc aatgagcgag tttttcctga ccctgcaccc gcaaaaagaa
     2761 aaaaactttt tggcttgtca aggtcgagac aagtgtagat ttcatcatca acggatttta
     2821 tgtcaattat atctgtcatc gctcaccctc tggcataact aaagaaggag ttcataaaat
     2881 cgcgtccctg attaacatag tattcagcca atcaagacct tctttaatat atgcgggagg
     2941 tttaagcaaa ttgggctccg tggtataaag taattccagg gccatttcag ctttcttagc
     3001 attcttgcca agcgcaataa acatctcctc gcaagcttca tctagagttg ctttagtaac
     3061 ggcgtccgcc attttcttta agagaccttt cctatccgtt atggctttaa acatcgtgag
     3121 attttccaac actagagcgt cttcaaacgt gtagggaata gcttcttcat tttttcctga
     3181 gagatattga atttttgtaa gacagggata cgccacccga accaatcctt cggcggttgt
     3241 tttttctttg ggcgttgcgg ataagacatc atctaatgtg gttttttttg gcgcccagca
     3301 ttttaatgta tcgtttcccg ttctaaggct ttggcccttc ttaggtcgtg ctttaccagt
     3361 agattctttc tcaattgaat caagatcggt gataacaagc gtgggaatac ccaaagcctc
     3421 aattagaggg cgtaacctgt gtgcgtggct cccgccaatc tccaacatgg agatataact
     3481 tctctctaac tctggatagt gatttctaat aaagtgggga accagcattc gttcgaccgg
     3541 cccttctacg agaattgcag catcagcaaa gaataaatcg cagtgagttg tttttaagta
     3601 acgactcgca aacttcgatg tatcatttcc ttcaccaaat atattgctca tgctaacgac
     3661 tatggcgcag ggtatttcta gctcgcttgc cagcagtttt ctccggaaat agcgtagtcc
     3721 ggtaaagtct gtctcatgcg cgatgtgact agaatgcgta ctcaccacca tttgactaga
     3781 aaattgcggt ttctctccta attttggttg attacgcaac acgccatatg ccttcttaat
     3841 aaaaacctgc tgcacttgtg cgtgtaagtg tgcttccggc tcttcaatga gcattaagtg
     3901 caacggctct ataggcgtat taccaatttt ctctcgctta cctacttttc caacacgcat
     3961 ccattcatca cgaaagcgga tcaggttaaa aatcatagaa atcaggtttt ggtagcccaa
     4021 accattgtat ttttcaggta ggcataggtc gttcaccgat ggatcttcac ttcgaacaac
     4081 tttaaattga acagctgagt catgatttaa cccttcaaca ggattcactt tacttgacag
     4141 tacaatctgt gggtcactaa accctgggta attcagacct tctagttcac caatagcgac
     4201 gctaaaactg ctcttcaatt tctgatcaaa cgctgttctg gcagcttcca tggcctcaag
     4261 tgcatccaaa tcacttgctt ccggtagttc gctagggtct agatgctcat taaagtaatc
     4321 tcttaattgc gaggagaggc ggcggtcagt attggcagtt ccctcgcctg aattcggatc
     4381 agagaatcct ctttgagcgt tgatgatgtt gattttgaat aggccatcaa aagggtcgct
     4441 ttcaagcggt tccattaatg ctgttaacaa ctgaggtgga tctgatgttg cgtgtgcggg
     4501 atctaacaca taagcattta cagagaagaa gacttgtagc tttctatcta aaaattcacg
     4561 catcgacgtt ggccatagcg tcaatgtaga taaagactca ttatttttat tttcttttgt
     4621 gcccttgcgc gccgttaacg tttcttgtgc tgctgaatat gcggccatat aggatttata
     4681 aagggtttct ggatcttccg gctccagtat cagcctgacc cccaacaagc caccatccca
     4741 gtctaatgta ggcagaatgt ggctaatgca gtgaatttga aattcatcta cgtttagcca
     4801 aacatcgata gctggtaaaa gtggacgcca agcatttaga gataaatctg ggggttctat
     4861 agaagcatta gtgacccagt cactagcaat tttattaatt gctgcccaat tagataatgt
     4921 tatatctgta gtgcttaatt ctcggcgctt tgattttttc agaaacataa tcagcgcatc
     4981 catagcagag gttttcccgc tattgtttgc accgacaaaa atagtacttt cacccgccag
     5041 ttctacacga cagattttta gcttgcgaaa gttttgaatg tcaatatggc aaattttcaa
     5101 tttaaaaatc cttatatccg attgctattg tatatggctt agatttccca tgggaaattc
     5161 accaccattt aaccgcattg ctaattttaa atctgttgcc gtttttaacc aggtcgctaa
     5221 cctacaagtg cttatgtgtt tgccggtggc cgaatttgcg aagtactccc tacataaaat
     5281 ctgcttaaag gctattgcat cgaacttcgg taactggaat ctaagaagca gtcattccct
     5341 cagccgcgca tattctaatc agtctgctcc ttgatacagg ctaacctatc ctcagcaacc
     5401 ccatccgcaa agtatcaacc aaatattcaa taacctagaa aaattggcct gcggttttaa
     5461 acctttgtca gcctgcgagc cattcaacta taaaattaaa gaattccact caaacttgtg
     5521 tacgccattc cttgaccaat ccttaaattt acagtgctac ctaaaacctc ccttagcact
     5581 tgctagtgca acaaaagttt aacctcatca caaaacccgc caagacataa gaccattgac
     5641 tagcgtcagt ttggccaatc aaatcagcca accaaccaac caaccaaccc gaatattaaa
     5701 ctcgataata aacaggctag atttaaaagc aatcacaaat tcacactcaa gcttaggcca
     5761 aaggtgctcg agtgcacaac ggctcgcccc catcacattg gaacgccaag gcgtatgtgc
     5821 ctacaccgat gctaaatggg gttgcccact atttcaggtg gcagtaagcc cagtggctcc
     5881 cccaaaattc tattgggcga ccgcaagggt cgcccctacg atattgcccc gagcagaggc
     5941 tcaaatccct gcgcagcatt aaagacccac aggcgtaaca cctggagaac ttccaagctg
     6001 atgagcacta cccaacagtg taattcgaaa aatggctggc gttttattaa gaggtggcga
     6061 aaaagggcct gggtgttatt cccaaacccc aagtaaaaac aaaaaagccc ggacgcaaat
     6121 ccgggcttag aagtttcaac agcagagcgc gatgagaaat aggcctaagg cgtgtaccgc
     6181 cacacctcaa cccaatcgta cacagcttta gcattaaccg tttcgctagt aaccgggcct
     6241 gcccaactgg gtgagttgga tgcccagata gtgagtaaaa cattttgcgg caggccgata
     6301 agattgatgc gcgaattcca gcggtggtac accacatcgt caattaaaaa tgtcgcttct
     6361 ttgggtgtcc attctatggt gtacacatga aaatcctggc tggaatcaaa gcctaattgt
     6421 tgtaatgagg gctcttgggt gggggatacg ggttttgagg tgggtgggcg cgctccggta
     6481 taaaccatgg cattaaattg gatggcgttg gggtttttgc ccaggctttc cgtatcgaat
     6541 tgattgccat catttgcagg ccaagggtgg catttacagg gtggatgcat aagacccccg
     6601 cacctgttgc tcaataactt aaagcccgct gcggtgagac cttctgtgca tagactggac
     6661 gggaagctgc gatagcctgc gagtaaaaac tcagccccgc tgccgaaggc cggcttttta
     6721 tttcccagtg ccaaaagctg cgcgcgaagc cttattgcac agcggccatt catccggtca
     6781 tccatccggt catccatccg gccattaatg cggccaatag ttggcttaaa agcctccaca
     6841 attttaaaaa agtggtttac tatcaataca ttatttatta agtcatgcgg ccaaactaac
     6901 cggccagctt gcgcccttga cgcttgcagg agtcaaaccc tagttgtcgc caatatggcc
     6961 ttcctgccac cacttctgcc accaatttaa tggtgattag atttttccag caacggccaa
     7021 taaatagccg ctaattcctt taccacttcg tctagcggcg gtgcttcgag tttgcttcgc
     7081 ttcaaaaacg cattccactg ttgtgccttt tcagttgcgt agcttttagt gagcccgact
     7141 ggaattgtag tgggtaagga tgtttgccgt cgcttaaagg ttgtggcaat cgccttgggt
     7201 aaaacagcgt ggtcgatttg ttctgctgtc gataacaccc acaaatcaaa ataatctttt
     7261 aggcgggtat tgaagcgggc caatttaatc aacgcttcaa gcttttccgc taccactgtc
     7321 tctaacggat aggcgcgcaa tacaggcgca ggcagatcca gtagcgttgg aaattcgctg
     7381 gaaactgctg aggatgttat cgcatcacca aacccaatat cgcattggat gggtatgcgg
     7441 gcgttatcca ggctcgcttg taattgcacg cgaatacccg gatagccagc agcttttcta
     7501 atcggttctg cggtgatgga ttcagttgaa aaaatcaggc cgtcttcggc atcgatctct
     7561 atcaattgcg caaaaatagt tttgatttgt tctgggtcag cttcaccgaa gccaaggaaa
     7621 tctatatccc gcgtaggacg ctgtggcaaa tcaaaccaga gcctgaacag caaggcacct
     7681 ttaagcacaa atcgatcaga atagtttgaa atggaaagcc gatagagcaa ccgttcaagc
     7741 gcaaaattta ctaatacctg ttgaaagggt tcgtggtgtg tcttggaaaa attgagtagt
     7801 ttttgccgaa tggaagccgc tagattgcca atggttttcg tcatacgatc aatgcctcaa
     7861 cataaggctt tagcacgttc tgcaccctgc aaattttggc cattgcatcc agcttgctaa
     7921 ggttgagtcg gttgctggcc caagcatctt tgagggcttc cagtgcaaca tccagcccga
     7981 cgcggttacg gaacttaaaa caatccgcga gggttttttc aatggaaaac acccgcaaat
     8041 tgccgccctc cattgcatgc acctccactc cactgtttag cgcttctgcc cccatatgaa
     8101 cccattcaag aggcggataa tcgctttgct tgcgccacgc attacttgca accgccaccc
     8161 ataccgctgg tggtgattgg gtgcccagct cgtgaaaagc cagtgctgag gctaggcaga
     8221 tcaccccgtt aggcaccaga tgctgggcag ccagccaagg gttttgctgt tggttgtctt
     8281 ccgccaactg gtagagcccg cgtccaacct tcacaacaaa accctcctgc tgccaacgtt
     8341 gcagcaccct aaaagcttgg gcatggctta gcccaaggcg gctgataagg tctccggttg
     8401 tatgaacctt tcctttctca aggatggcat gaggctgagg aggcatgtga gacaagccta
     8461 tgttattaat tgaatataca tagggtactc tcatatgggt tgaaagcaat ataaatctat
     8521 tgtttatagt ggtttttgtt ggaaggctgc cttcctgccc accacttctg ccacccgttt
     8581 tcaacaacag gccaaataga ttttcccgct ggaggcagca agcgatcact gcgttcataa
     8641 ttacgccttg ttcatgaatc atgagcgtga tagtttaatg aacaaatgcc ccagagttat
     8701 ccaacgtgaa cagcccatca gaatctgacg ttttaacgca agcgctactg gctctaaagg
     8761 gagagaccgg gatggatttt tccgtgagtg gacaggtgcc ttcgagcagt gaagccgact
     8821 atttgatcca gacgccatca caatctctag cggttgaagt taagcgcaac attcggccag
     8881 cgcaccttgg cgctattatt cggcgcatca aaacactaga gcctacaggg ttgctggttg
     8941 ccgactacct caaccccaat attgccaaag tgttgaagga aaatgatgtc caatacatgg
     9001 atgcctgtgg caatagctat ttgaatttgc ccccaattta tgtacatatc tccggccaaa
     9061 aacctgtact aaacacaaaa caagagacca acaaaacatt tgatctcagc ggtttaaaag
     9121 tcatctatgg gtttttgtgc aatcggcaat tggttgacgc aagctaccgt gaaattgctg
     9181 agcaaactgg cattgcgctc ggtgctgtag gaaaggtctt aaagagtttt gtaggcgctg
     9241 gctttttaat tgatcaggga aagggtaaaa agcggcagct tattaatcgc cgcaagttat
     9301 tggatcgctg ggtagaagct taccctgaaa aattaaaacc aaaattattt attggggagt
     9361 ttattggtga taacggcaat tggtggaaaa acatagaaat tgaaaaatac gacgcttatt
     9421 ggagtggaga agttgctgcg gctaaataca ccgactactt aaagccacat gtggccacct
     9481 tgtacgtacc tgaaaaatca ggcagcaaac tttttgccgc tgcacgctta cgtaaagcga
     9541 ataacactac taacaccatt gatagactgg tgcacgtata ccgccctttt tggccggaaa
     9601 gacaaaatca tgaacacacc aattacataa gagcaaatac cgttcacccc attctcatct
     9661 acgccgattt aattgcctcg gcagataccc gcaacttgga aaccgcaaag aggttatatg
     9721 acagcgccat cgctgaatat atcgcagaag attgagccct cagtagcgga tctctgcgca
     9781 atcgttgcag gctgtgccat gagattgcaa ataccttact tagttgtagg tgcttctgcg
     9841 cgcgatatgg ttttgcatta tggttacgca gccaaagtac aacgagctac cgctgatata
     9901 gactttgcac tacaggttcc gtcctgggcc gcttttgcgg ccttgagaag agaattaata
     9961 agaaacaatt tttctgagac cattgcaaca caccgattga tagggccgaa taattggccc
    10021 atcgacatag taccttttgg tggcgtagca gatgaacgcg ccaatattca atggccgccg
    10081 tctggtgact ttgtgatgag cgtgctcggt tttgtcgaag ctttaaatca cgcaaatatt
    10141 gtgcgtctga gtgatatgcc catacttgat gtgcccgtcg cctcaccaga gggaatgaca
    10201 atgcttaagt tcattgcgtg gactgatcga gctgcagatg ccagaaaaaa agatgcaaaa
    10261 gacatcgcct acctgctcac aacttatgaa aacattcctg ccgtttcaga tcagttatat
    10321 cgcgaattga cactcatgga actttatagc tgggatataa ccttggccag tgcgcatcag
    10381 ctaggaataa atgtaaagca aatggccttg ccgcaaactc ggagtgttat ctcggacgtg
    10441 ctaaataaac agagcgctaa actatcagtg gaaaaattaa taaatgaaat gtgcgaaaat
    10501 ccagaccaat acgctcgtaa tgcaatgttg atagatgctt ttggtgcggg atttttcagt
    10561 taacgcagat gggttgattg tctttataca attcgcacgc gtttgtgtac tttgtttaaa
    10621 gctgcgcgcc tagccttatt gcctaagggg catccatccg gccattaatg cggccaatcg
    10681 ctggcttaaa agcctccgca attttaaaaa atgttttatt atcaatacat tatttatcaa
    10741 gcccggcgcc caaccaagcg gccaaatggg ctcctttatg gggtgcgcta tggctacatc
    10801 gataatggat attaggccgt ttatccccag tgatggatcg ctcgcgcaat cagcgttacc
    10861 tgataaagcc atagcgctta taagccatgc ggccaaactt accggccagc ttaattttat
    10921 atacggcatt cgcccagggc gaaattgaac gcgcgcgcat tggaattatg cgcaatgcct
    10981 gaacgcagcg cacggcgttt attgagccaa ttaaaaagcg aaggtttgtt gagtgaaact
    11041 agcagtaaat cgccgctgcg ctgggaaata cccgaacatg ctgaaccctg gtattttcct
    11101 aatttgacac tctaactcct gcattgaggg gctaacccag attttccgca cccaaaggaa
    11161 aaataaaaaa gcccggatac aaatccaggc tcttaagttt caacaacagg gtgtaagttg
    11221 aattaaggcg tataccgcca aacctcaacc caatcgtaca cagccttagc attaaccgtt
    11281 tcgcttgtaa ccggccccgc ccagctggtt gaattagaag cccagatagt gagtaaaaca
    11341 ttttgcggca ggccgataag attgatgcgc gaattccagc ggtggtacac cacatcgtca
    11401 attaaaaatg tcgcttcttt gggtgtccat tctatggtgt acacatgaaa atcctggctg
    11461 gaatcaaagc ctaattgttg taatgagggc tcttgggtgg gggatacggg ttttgaggtg
    11521 ggtgggcgcg ctccggtata aaccatggca ttaaattgga tggcgttggg gtttttgccg
    11581 agcatttcca tgtcgaattc attccaatca tctgcaggcc aaggggtata aatggagacc
    11641 aaggccgaaa ccacggcgga gcccttggca aatttagccc gtgcgctgac gcggccataa
    11701 tttatggtgg cagtggagcg aacttctgca cctaaaaatg atttggtttc accgtctacc
    11761 actgtgccgg aaggggcagg ggcgagttta atggtcatca tgccgttgct tacggcaacc
    11821 gtatctttag aaaacagcgc caggttgccg gcccaggaat gggtcattaa ttgccagcgg
    11881 ctggaatcaa aggaattaaa atcatcgcga aacaacagag taaatttact gccggaagaa
    11941 ctgctggacg agctggatga gctgcttgaa gaactggatg aagagcttga tgaactgctt
    12001 gaggaagaac ttgagctgct tgacgaggag ctggtggagc tacttgaggt agagctggaa
    12061 gagctgcttg aagacgaact ggatgaactg ctgctggatg tattgccacc acaggacaca
    12121 agggatgtaa tcagcagtgt ttgcgctgct gccagtaaaa tttggattaa atggggtttg
    12181 cgcatttttg ggccctgtat ttttatttgt ttgggtattt gcctggcgcc aggcaggaat
    12241 aactggcgcg ccccagagtt tggcggcgca aaaatgccgt gtaaatgagc agtttatgtt
    12301 ttgaatgaag tggtacgagg cattgcagcg gaattagggc ttattaaccc gcgccggata
    12361 tcattcaagt gcgttaaaac agaggagcct aaagtggctt ttgatgttgc tcacgaaatt
    12421 gtgtaggcgt catatcggta aactttttaa aaaaccgatt aaaagccgct ttgctattaa
    12481 atcctgcgtt ggtggctata tcggtgatgg ccagggcgtg tgaatcgggt gcgcacaaca
    12541 cgcgcttcac ctcctctacg cggtagcggt tcacgaattc gtggaagttt tgctcgagct
    12601 tgcggttgat tactgaggaa accaaacgag gatgcagccc ggtttgtttg gagaattctt
    12661 ccagcgtaag cctggagtta aggtaaagct tatctttttc catcgcattt tggatgattg
    12721 ccagctgctc tggggttatt tcatccctgc tggtgttttc gcctgcgctt tcattttcat
    12781 ttttgccctc gttaaccccc tcgaacagat ccgaataaat taggctgtaa aaaattagga
    12841 tattaataag gacaaagagg acatagttgg ccgaaacggc aaggccttca cccattggcg
    12901 caagcgcaaa ggcaccaaaa atatgcgcgg ccagaaaaca cagccaagag gccataaagc
    12961 cgcctatcaa caacattagc caggtcaggt caatgcgttc aatgtcggaa taattgtgtt
    13021 taagcagttt gcggtacttg aataattggt aaagacaagc gattccgtac agcactatca
    13081 cgatcttttc cgggtgcaca atccagtcaa acagcggttc agtagcgcca tagtctttaa
    13141 aattgagcgc tatgccctgt ttaatttcat gcgggcggca gtaatacaaa aaatacagga
    13201 ttatgggcgt tgccgctgcg ggtattagat gcagaatgtc ggtgggtgcg aacttaaaat
    13261 ctttgtagag caccgagcgg atataccaat aaagaatcgg cccttctaga aagtaggcga
    13321 aattaaagat aaaaaataca ttggggctaa agtcaaaggc cagaaatcga aacgcttccc
    13381 cccaaagggt gagcaagtgc aaggcaatca tcgcattgag cagcaggaac agcgccagta
    13441 ataaattgca gtagggtttg gcgccgcgat gggctagaaa caacaccgcc agcaggctgc
    13501 actcgccaac gcgcattagc agggccacat cgtggatatt aaaaagaacg gcgttcatgg
    13561 cttattgtta gatttatgga atatggcgtg aagctcagga cgtgaattgt gccttttcct
    13621 ctaggcgtat ggctatgcgt atttttaatc gctctataaa tgtgcggtgt ttaacaaaat
    13681 gttgggtatc ggttaatgca tttgcaggaa atggtacgtg aggtgtgttt tcttatacgg
    13741 cctgatgggg ttgctggtgt gcacaattgc ttgcctatca cttgctctct tctctacaac
    13801 tgtctttcaa acggcccagg cttggctgac ctcgccctca ttaaaggatt tgagcctatt
    13861 ttcatgccct tttccctccg ctagaaaccc cctcccggcc tcccccttat tctgagatga
    13921 gcatcattag ttttttattt gaaaaagacg aatttcaaac ggcccaattt ttggttggcc
    13981 gccgacaata attttccgat cttttacatc aagtgcactt taatcgtggt taaaggaatg
    14041 ggcgcgacct tcaggcctgc aggtgaggct gctggctctt cccaagtgaa aaaaaaccgc
    14101 agctattaac tgcggtttta attaacccat aaaaacctta agcagaaacc acatcatcct
    14161 gcgccacaca taattgcgcc tgcaacttgg caataatggg ggcgctgacc atgccgtagt
    14221 ggttgcaggc caaaagctgc tcggtgcggc cagtggggat gaggtggcgc cagatggtgg
    14281 cggactgggc ggctacgcgg gtttccactt gttgctcggc catggccaca aagcttggca
    14341 cgcaggcggc cagggtgctt gcggtatagc tgtgcagcag gcggatattg gcgcagaagc
    14401 aggcgtagat atccttaagg cccgccaggt tgccccgggg caaaaggccc tgctgctgga
    14461 tttccctgag tactgcctca agttgcgggt tggcgcagtt gagggttggc agggccggta
    14521 gtgtgtcctg ttgtaataga tcgcttagaa accaggtgag caggccctgt tcatcgggca
    14581 ccgggcgctc caccggggcg gggctgtcca gcagccacag ggcggccacc tgttcaccca
    14641 cctggataag gtgttgggcg caggcgtagg cgataacgcc acccatggac cagccgccca
    14701 agtaataagg cccctggggt tggcgcgtgc ggatggcggc taagtagctt gccaccatgg
    14761 cgttaaagct tgtgaacata gcctgcttcg cgcctttggc cgcctgtacc cccactaggt
    14821 taaagcggcc agctagggcg gtggctaagt cttggtaaca gagcacattg ccgccggagg
    14881 ggtggaacca gaaggcattg gggcagtttg ggttcctcgg cagcaggtcc accaggtggc
    14941 taaaggcccg ggcctcttga tgctcggcct gcaacagggc ggcaaggtcc ttcaagatgg
    15001 gcgcctgcac cagttgagta atggggatat cccgccctaa ctcttcccct aaggccatca
    15061 ccaagcgcac ggcggcgaag gagttgcccc ccagttcaaa gaagtgatcc cccaaattga
    15121 cctcggccag gccgagaatc tcgcaccagt ggcgggcgat taggcattcc atagggcttg
    15181 ccggggcggc ctggctgcgg ctgtggctga ccggctcggg gttgggaagg gcggcgcggt
    15241 tgattttgcc gttggcgctt aagggcaact ggtccatagg cacccagcgg ctgggcagca
    15301 tatagctggg gacgaatcct gcaaggtagc gggttaaatc ctcaatagtt ggcggatgat
    15361 cggcggcgag caccacatag gcggctatgt aggcgtcttg gggtgaggtg ccgattacct
    15421 tggctacggc accttgcacc tcggggtggc gctgcagcac cgcttcaatt tcccccagtt
    15481 caatgcgaaa gccgcgcact tttacctggc cgtcttcgcg gcctaaaaag tcgatgtacc
    15541 ctgcctcgcg catcacgc
//
LOCUS       DAOQGS010000016        15497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1755_length_15497_cov_4.534128, whole genome shotgun sequence.
ACCESSION   DAOQGS010000016 DAOQGS010000000
VERSION     DAOQGS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15497
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_15497_cov_4.534128"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /gene="fadA"
                     /locus_tag="PKZ52_01220"
     CDS             complement(<1..351)
                     /gene="fadA"
                     /locus_tag="PKZ52_01220"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602325.1"
                     /note="FadA; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadB; similar to
                     FadI2J2 complex; functions in beta-oxidation of fatty
                     acids; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNG58414.1"
                     /translation="MTDSNRDVVIVDYCRTPMGKAKNGCFRFQRADDLSAHVMKSLLE
                     RNPGLAPKDIDDVIWGCVMQREEQGFNVARNSLLLAGLPHSIPGQTVNRLCGSSMSAL
                     HAAAANIKAGVGDVY"
     gene            complement(361..2502)
                     /gene="fadB"
                     /locus_tag="PKZ52_01225"
     CDS             complement(361..2502)
                     /gene="fadB"
                     /locus_tag="PKZ52_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HNG58415.1"
                     /translation="MFSGQSLSLTRLENGLVQLTFDLQGESVNKFNQATSQELKAALD
                     ALSAEPGVRGLLLTSQKSVFFVGADITEFVPLFVQGSEAISKALRFNSQQFSRLEDLP
                     FPTVVAINGYAMGGGLEVCLACDYRIMASTAKIGLPETKLGIIPGWGGTVRLPRLAGL
                     DTAVEWIASGSEQTAAQALKARVVDGVVAPELLYQEALACLTALADGRLPYLPRRAHK
                     KAPLSLNNTESLLAFESSKAFIKAQAGRNYPAPVAAVKAMQEAAKLERDEALDVELKH
                     FVEMARTSVARALVGVFLADQQLGRKAKHWEKHADKKVAQAAVLGAGIMGGGIAYQSA
                     SRGVPIKMKDIAQAGLDLGINEATKLLSGRLEKGRITAGDMAATLNRITPCLDYQQFN
                     GVDIVVEAVGENPKVKRAVLQEVEAQVRDDAVLCSNTSTISITHLAETLKRPENFCGM
                     HFFNPVHAMPLVEVIRGEKTSDTTVARTVAYANALGKKAVVIKDCPGFLVNRVLFPYF
                     SGFSLLVKDGVDFQTIDGVMERWGWPMGPAYLLDVVGLDTAMHAQDVMAQGFPDRMQE
                     GYESSVVQLYKASRLGQKNQKGFYQYAPDKKGKPQKAVDPEVYGLIGQPHLKITDEAI
                     IARMMIPMVTELTRCLDEGIVESAFEADMALLYGLGFPPFRGGLFRWLDEIGLKQLFA
                     MAKPFTHLGPLYELPPSLQARLDQNQKFYSL"
     gene            complement(2691..3260)
                     /locus_tag="PKZ52_01230"
     CDS             complement(2691..3260)
                     /locus_tag="PKZ52_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG58416.1"
                     /translation="MSQALGLGEKLRALRQAQGLSQRALAQLAGLTHGNLSHIEQGKI
                     SPSVLTLEKILNALNIPIASFFAMAEPRAILPGADWPGAQEAGVHWSFMEVLAAASPT
                     YLVVHRLQPGAVQSRDFLRHRGVLAGCVVSGRLELSLDDTQHNLAPGDGFYFSHQRSF
                     RLTNKDSTLATWLGVSLLPAQLQPPVSAE"
     gene            complement(3257..5155)
                     /locus_tag="PKZ52_01235"
     CDS             complement(3257..5155)
                     /locus_tag="PKZ52_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HNG58417.1"
                     /translation="MANLPVIVGFGGYNAAGRSSGHRAYHRMVFESLDQRAQQETILS
                     LAALMKIAVWQEGTWTYQGNPCSAQACAQLCREAVLQGTLIRRIEANFFDPDRAVANK
                     SVRWEVAEGADVFILANKNIPEPLPADWIVEAIDDKTSRVRLTGPLDLFLPCAAPMAV
                     QAAGQLPSGFNPGDHYKSLNHPRGLQLAIMGASDAIHSVGIDWQTIVNAVQPDEIAVY
                     SSSVMSQMDSTGFGGMMQARTLGGRPTSKQLALGLNTMPADFVNAYVLGSVGSTGGVT
                     GACATFLYNLRIAADEIKAGKRRVALVGSSEAPIIPEVIEGYSAMGALATDANIAKLD
                     GSETADHRRSCRPFGDNCGFTIAESSQYVLLMDDALALELGADILGAVPGVFVHADGY
                     KKSISAPGPGNYITLAKAVGLAVQLLGEDAVRQGSFLQAHGSSTPQNRVTESRIFDKV
                     AVHFGIERWPLAAVKAYVGHSLGPASGDQLACTLGVFAEGVLPGIKTISAVAPDVAQG
                     RLAISNQDQKLTKPQAAFLNSKGFGGNNASALVIAPEAAFKLLAKRYSPAAIAAYESK
                     RAETRARAEAYFAAADKGDLQAIYRFGEALLQEDDLSFSEQGLHIKGFEHSIGLSSAE
                     GFEDWIKP"
     gene            5267..6256
                     /locus_tag="PKZ52_01240"
     CDS             5267..6256
                     /locus_tag="PKZ52_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG58418.1"
                     /translation="MTSVTFFLCDEMLASSITLPLEQLSAAADFSLAHRKKNNSPSFD
                     IRLASLDGLPKRAHSGVHLAAHCEITNIEHSDITYLPALWRNPQKLLKHNRALLPWLQ
                     EQHNRGNIIAGVGTGCCFMAEAGLLGHQTATTHWHYFDEFAAHYPKVKLLRDYFITRA
                     GNLYCTGSVNSTADLTIQFIRHYYSAAAANHVERHFFHEIRNSGLHVGSPQWDQPHRD
                     ELIAQAQAFLGEHLESALRMEQVAQHFGLSLRSFNRRFKAATAQTPLNYLQERRILLA
                     KELLKTSNLSIGEIALRCGHQDFAHFTGLFKKNLGTTPSQYRTTVRAKLFAVA"
     gene            6603..6917
                     /locus_tag="PKZ52_01245"
     CDS             6603..6917
                     /locus_tag="PKZ52_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNG58419.1"
                     /translation="MKSSADVVYRDFDSSPALTSIIEKRLEKLGRFSDSIMSTRVVLE
                     SPHNHKHKGKMFRACIEMAVKGSPVTVTHDDPSVHVAVRDAFQAAERKIKEVEERRRV
                     SH"
     gene            7290..8651
                     /gene="yegD"
                     /locus_tag="PKZ52_01250"
     CDS             7290..8651
                     /gene="yegD"
                     /locus_tag="PKZ52_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HNG58420.1"
                     /translation="MFIGLDFGTSQCAVTAATPNGLVSVPLEAQQPFLPSCIYTPERC
                     LISDFVAAAISQEKHEYMQSRHTPLQLVPETKRNLSIRHSREALLFGTDAMAQHMADP
                     IDGFFIKSPKSFLGANGLREDMVSFFEDVVTAMLLEIKQRAEAYLQHNITGAVIGRPV
                     NFQGINAEASNRQAQGILSTAALRAGFTQVEFLYEPLAAGLDFERKLTEDKTVLVIDI
                     GGGTSDCSMVRMGPNHRQKVNRQRDFLAHTGERIGGNDFDISLAREALMPYYGMNSPL
                     KNNLPMPVLPFFQATAVNDVAAQSAFYSDKMRLILEQLSRDTIEPDVFQRFIHLRENK
                     QNFALIRKAELAKIALSHNNNSEVDLTAYTTGLIVDIQHQQLVAASQILLDKVKHLAQ
                     EAINQAQQIPDLVYLTGGSAKSRLLKETLAQYLGTQMPLIEGDHFGSVVSGLGVWAGK
                     LAT"
     gene            complement(8986..12408)
                     /locus_tag="PKZ52_01255"
     CDS             complement(8986..12408)
                     /locus_tag="PKZ52_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58421.1"
                     /translation="MSYKNLKKPNFRFLPLLALAFSLGLAGCGSGGKKGEGLLGEPVP
                     KPPVTSSSSSGAASSTSSGGSTVGSYTDSETGSTVNVTVASEEGEVAFGVSKKITLTF
                     KDSAGKVFEPKGISVEASSNCITTLKGATISTPTLNSNVMTFSYTNQGCRGDDWVYFT
                     SGTGTAVAKLGSVKMVTIGESVASIQFIKADPSKIYVAGDSTPKESVLTFKVLGQGGK
                     PLENEPVVFSNSGTSGGITLITSSDNSDKDGLVEVRVKAGTVPTTVSIKALHEKSKVE
                     GFSGGLEVSAAMPVESTFAIGATKYNIRSYGRINDETTQIVARVSDSAGGYVPDGTTV
                     RFETREGGSIESCKTVNGTCTSTWIPSKKQPLDGRVQVMAYVQGVEEFKDNNSNNVFD
                     DGDTFVDLPEPFSDDNQNGQYDIGEFFADANNNGKRDVADGKWNGVNCKHSSLCAPAS
                     QLVNLSKQLTLFLSYGAFEICEPGDFAKTIWVKPKGVTTVGGMFISDGNGSASNEGHP
                     CKTGNPLPNGTKITFTPSGGSLKGGSGVFTVGADEGLPKGPLGVIYEAPDQAGPQFLT
                     MKIEIPGMLEGSRTWPIVVSDTPPDDSNNIPDANTAGTFIDPTLNQKVWVDIAAPAGQ
                     VMFDTSKEVTLTFKDEQSLPKTLTSAFSATSACIVSSRSQISAPNVSGNTVKFTYTAK
                     GCVGDDVVTFNAQGGGKTVTVGSYKFSAAGEAVGGITFVSASPTQIPISTTGSGSGVF
                     ATVTFKVLGQSGNGVAGENVRFSIVGEAGGVELVTLAAVSDLNGLVKTQVKPGTTPNN
                     VTVLATVESNGAKASSLGLAVASGLSAPGRFSIALSSFNPHAYNFDSGIDAVTVSATV
                     ADASGNPVIDGTLVNFVSPEGGLITPTCLTVKGRCAVGFQSTGIQPKDGRLRIAAYVK
                     GVEHFIDVNGNLIFDDNDIFDLNSDDTYEPYVDENDDGSYQLGEYFVDSVVNGVRDGP
                     DGKWNGLNCAHSSLCGAAKRLVDIQDTAVVNLSGYSGPTICQAGTFGNGSVPLTVAPG
                     GTLSLSGLYLSDGNPFAVNLSGACATGNSLPGGTKVTFAVSSGATLLSPKDPLTVTPY
                     ERQPNGSYYAVFTAPTTPGVVVLTMKIEVPPYVGSKDTAAYIYNYNWTIDVK"
     gene            12662..13318
                     /locus_tag="PKZ52_01260"
     CDS             12662..13318
                     /locus_tag="PKZ52_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HNG58422.1"
                     /translation="MYNLTPHEARVIGSLIEKALTTPDQYPLSLNSLTMACNQKTNRE
                     PVMELPEAQVQSTLDDLVKKHLVSPISAMGSRVVKYQHRFCNTQFSELQLSPGELAVV
                     CVLLLRGWQTPGELRTRTQRLHDFNDVLDVEKVLHHLLERSQPLVCRLEREPGKREHR
                     YAHLLGSDIHEQAQSSTAVPPAAERKQDELTALKEELAQLREEYVKLSERVSVLEGLF
                     "
     gene            complement(13451..>15497)
                     /locus_tag="PKZ52_01265"
     CDS             complement(13451..>15497)
                     /locus_tag="PKZ52_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNG58423.1"
                     /translation="IASRAEVIAKEEEKIALEQQARQFVDAAVNDMQNLLAKLYTATH
                     MDELKAMDVPKKIQELTQAISLAARNIHAAEQVKLFNDYKERAHNLFNKLSESGPVVQ
                     VLNQLQEAGNSETAHNLKRHVQAILQEAKDFQHEQVTPWLIETKQQFNAWLDTQKDKE
                     DQARQLVKDLADLTRKGVWAAEQGMVRKARGIYKDVQEKVQQAAEELPANITGKLQDF
                     EHLMQKLGDWHEFAVTPKKEDLIKQMQALSESKLAPNDLASKIHELQDQWKELSRGGQ
                     QNDESLWEQFQAASVQAYTPCKEYFERQTQERESNSAKRTGLITQLQAYLEQYDWQNA
                     VWKDVEKTLKLAREEWKTYWPVPRKLVKEQQAQFDALLDQLHGKIKTEYQANKNWKEK
                     IIAEAAELKDAVDLAAAAEKVKQLQQQWKSIGRSWPKDDQTLWDDFRKHCDAVFEKRK
                     QLYDEVNAQRDAVVEKAKDYLNQLKTWVESAPDAVASAKTHIDELKTQFEGLGELPRK
                     AAAELTSQYHHYSEQIKQALQQARSFAQAKAWNDVFNLADLLRRHECNRLASDDSCGD
                     SPELEAAWAAINSWPAGTQDVLSQRKQLGLDALRQKSQDAENQLLLLCIRAEILSQKD
                     TPEAFKAQRMAYQVEQLQLNFGKRDETMEALVLEWLATPAVADEAYQAAWQRFAACR"
BASE COUNT         3870 a         4140 c         3736 g         3751 t
ORIGIN      
        1 gtacacatcc cctaccccgg ctttgatatt ggccgccgcg gcatgcaggg cggacatgga
       61 cgagccgcac aggcggttaa ccgtttgccc ggggatactg tggggcaagc ctgccagtaa
      121 taggctgttg cgggcgacgt taaaaccctg ctcctcccgc tgcattacac agccccagat
      181 cacatcgtct atgtctttag gggcgaggcc ggggttgcgc tcaagcaggc ttttcatcac
      241 atgggcgctt aagtcatccg cccgctggaa gcgaaaacaa ccgtttttgg ctttgcccat
      301 gggggttcgg cagtaatcaa caatcactac atcgcgatta gagtcagtca cagcgcctcc
      361 ttacagagaa tagaattttt ggttttgatc aagccgcgcc tgcagagacg gcggcagttc
      421 atacaatggc cccaaatggg tgaagggctt ggccatggcg aataattgtt tcaagccaat
      481 ttcatccagc cagcggaaca acccgccccg aaacggggga aagccaaggc catagagcag
      541 cgccatatct gcctcaaagg cgctctccac tatgccttca tccaggcaac gggtaagttc
      601 ggtaaccata ggaatcatca tgcgggcgat gatggcttcg tcggtaattt ttaaatgggg
      661 ctggccgatt aggccataca cctcagggtc caccgctttt tgcggtttac cttttttatc
      721 cggcgcgtat tggtaaaagc ctttttgatt cttttggcct aggcgagagg ctttgtacag
      781 ctgcaccacg gaggattcat agccctcctg catacggtct gggaagccct gggccattac
      841 atcctgggcg tgcatggcgg tatcaaggcc caccacatcc agcagataag caggccccat
      901 gggccaaccc cagcgctcca tcacgccatc aatggtttga aaatccacgc cgtctttgac
      961 caacaaacta aagccagaaa agtaaggaaa taacacccgg ttcaccaaaa agccggggca
     1021 atcttttatc accacggctt ttttgcccaa tgcgttggca taggctacgg tacgcgccac
     1081 ggtggtatct gaagtcttct caccgcgaat aacctctact aagggcatgg cgtggacggg
     1141 gttaaaaaag tgcataccgc agaagttttc cggccgcttg agggtttccg ccaagtgggt
     1201 aattgagatg gtagaggtgt tggagcagag cacggcgtca tcgcgcacct gggcctccac
     1261 ttcttgcaga accgcgcgct tgaccttggg gttttccccc accgcctcaa ccactatgtc
     1321 tacgccgtta aactgctggt aatccaagca gggggtgata cggttgaggg tggcggccat
     1381 gtcacctgcg gtaatacggc ccttttccaa acggccgctg agcagtttgg tggcttcgtt
     1441 aatgcccaga tctaaaccgg cttgggctat atctttcatc ttgatgggca caccacggct
     1501 ggcggactga taggctatgc cgccgcccat gatgcccgcc cccagcactg ccgcttgggc
     1561 gactttttta tcggcgtgtt tttcccagtg tttagctttg cggcccagct gctgatcggc
     1621 cagaaatacc cctaccagcg cacgggccac ggaagtgcgg gccatttcga caaagtgttt
     1681 cagctccacg tccaaggctt catcgcgctc aagctttgcg gcttcctgca tggccttaac
     1741 cgccgccacc ggcgccggat agttgcgccc ggcctgggcc ttgatgaacg ctttggagga
     1801 ttcaaaagcc aacaaggatt cagtgttatt aaggcttaaa ggcgcctttt tatgcgctct
     1861 acggggcaaa taaggcaggc ggccatcggc caaagcggtg aggcaagcca gggcctcttg
     1921 atacagcagc tcaggcgcaa ccacgccatc caccacccgc gctttaaggg cctgtgcggc
     1981 ggtttgttca ctgcccgagg cgatccattc caccgcggta tccagccctg ccaaacgcgg
     2041 caggcgcact gtgccacccc aaccgggaat aatgccgagc ttagtttccg gcaggccgat
     2101 tttggctgtg ctggccataa tgcggtaatc gcaggctaaa cacacctcca ggccgccgcc
     2161 catggcatag ccgttgatgg ccacgacggt tggaaagggt aggtcttcca aacgggaaaa
     2221 ctgttggctg ttaaaccgca gggctttgct gatggcctct gagccctgga caaaaagcgg
     2281 tacaaactcg gtaatgtcgg caccgacaaa aaacaccgac ttctggctgg tcagcaacag
     2341 gccgcgcaca ccgggctcgg cgcttaacgc atccaaggcc gcttttaatt cttggctggt
     2401 ggcctgatta aacttgttta ccgattcacc ttgaagatcg aaggtaagtt gcactagccc
     2461 gttctctaaa cgggttagcg acagtgattg gccgctaaac atcgtgttat tgcctcgtta
     2521 agctgatggg atcgaaggtg ttggggacga ataacctcgc gcacaactga gccacaaaaa
     2581 atctggcgtt ctagccaaca agatggcgtg gcaaggagcg tgcaccattg tggctagtat
     2641 ggacgcttaa cagggtaaaa actagcaagg gcgccgccgg gcgggcaatt ttactcggcg
     2701 gataccggcg gctgcagctg ggcgggcaat agagaaaccc cgagccaagt ggcgagggtc
     2761 gaatccttat tagtcaggcg gaaactgcgc tggtgggaga aataaaaacc atctccgggg
     2821 gcgagattat gttgggtgtc gtcaaggctc agctccaacc ggccgctcac cacacagcct
     2881 gccagcacac cgcgatggcg cagaaaatcc cggctctgca ccgcccccgg ctgcagccgg
     2941 tgcacaacca aataggttgg cgaggctgcc gctagaacct ccataaacga ccagtgcaca
     3001 ccagcttcct gtgcccccgg ccaatccgcc ccaggcagaa tcgcgcgggg ctcggccatg
     3061 gcaaaaaaac tggctatggg gatattgagc gcattaagga ttttttctag ggttaatact
     3121 gaggggctaa ttttgccctg ctcgatgtgg gataagttgc catgggtgag gcccgccagt
     3181 tgcgccagcg cccgctggct caggccctga gcctggcgca gggcccgcag cttctcgccc
     3241 agccccagag cctggctcat ggcttaatcc aatcctcaaa gccttcggcg ctgctgagcc
     3301 caattgaatg ctcgaagcct ttaatgtgca ggccctgctc gctaaaactg agatcatcct
     3361 cctgcaacag cgcctcgcca aaacggtaaa tggcctgtaa atcgcctttg tctgccgcag
     3421 cgaaataggc ctcggccctc gcgcgggttt cggcacgctt gctttcatag gccgctatag
     3481 ctgcagggct gtagcgtttt gccaatagtt tgaacgcggc ctccggcgca atcacaaggg
     3541 cagaggcatt gttaccgcca aaacccttgg aatttaaaaa ggccgcttgg ggcttggtaa
     3601 gtttttgatc ctgattggag atggctaggc gcccctgggc cacgtcaggt gccaccgcgc
     3661 tgatggtttt aatacccggc agcacgcctt cagcaaatac ccccagggtg caagcaagtt
     3721 gatccccaga cgcggggccc agggagtggc ccacataggc cttcactgcc gctaagggcc
     3781 agcgctcaat accaaaatgc accgccacct tgtcgaaaat gcgggattcg gtaacccggt
     3841 tttgcggcgt gctcgaaccg tgagcctgca ggaaactccc ctgccgaacc gcgtcctccc
     3901 ccaacagctg cactgccaaa cccactgcct tggcaagggt tatatagttg ccggggccgg
     3961 gggcggaaat ggatttttta tagccgtccg catgcacaaa taccccaggc acagccccca
     4021 gaatatcagc gcccagttcc agggccaggg cgtcatccat tagcaacaca tactgcgagg
     4081 actcggcgat ggtaaagcca cagttgtccc caaatggtcg gcaagagcgg cggtgatcgg
     4141 cggtttcaga gccatccaac ttagcgatat tggcgtccgt ggccaatgcc cccatggcgc
     4201 tgtagccttc aataacttcc ggaataattg gcgcttcgct gctacccacc agggccaccc
     4261 ggcgtttacc ggctttgatc tcatccgccg ctatgcgcag gttgtacaaa aaggtcgcac
     4321 aggcgccggt gacgcccccg gtgctgccca cactgcccag cacataggca ttcacaaaat
     4381 cggcgggcat ggtattcagg cccagggcca gttgtttgga ggtgggcctg ccccccaggg
     4441 tgcgcgcctg catcatgccg ccaaaaccgg tgctatccat ctggctcatg acggagcttg
     4501 aataaacggc gatttcatcc ggctgcacgg cattcactat ggtttgccaa tcgattccca
     4561 ccgaatgaat agcgtcactc gcccccataa tcgcaagctg caaaccgcgc ggatggttga
     4621 gggatttata gtggtcgcca gggttaaaac cgctgggcaa ctgccccgcc gcttgcaccg
     4681 ccatgggtgc tgcgcagggc aaaaacagat ccaggggccc agttaagcgc acccggctgg
     4741 ttttatcgtc gatggcctca acgatccaat cggcgggcag aggctctggg atgtttttat
     4801 tggccaggat aaacacgtcg gccccctcag ccacctccca gcgcacggac ttgttcgcca
     4861 ccgcccggtc agggtcaaaa aaattcgcct caatgcggcg aatcaaggtg ccttgcaata
     4921 cggcttctcg gcacaactgg gcacaggctt gagcgctaca ggggttgccc tgataggtcc
     4981 aagtgccctc ctgccaaacg gcaattttca tcaaagccgc cagggacaaa atggtttcct
     5041 gctgggcgcg ctggtctaaa gattcaaaca ccatccggtg gtatgcccgg tgcccagagc
     5101 tgcggcccgc cgcgttatag ccgccaaagc ctacgattac cggtaaattc gccatagcac
     5161 acccacgaaa attagttagg tgcctagtgt atgggttgtt cggaaacaca cgcctaggca
     5221 tactggccaa ctaaccaagg cattcaggcc aaattgggcg gtaaagatga caagtgttac
     5281 tttttttctc tgcgatgaaa tgcttgcgtc gagcatcaca ttacccctag agcagctaag
     5341 cgctgccgcg gacttcagcc tcgcccaccg caaaaaaaac aattcccctt cttttgatat
     5401 ccgcctagcc tctttagacg gcctgcccaa acgcgcccac agcggcgtac acctcgccgc
     5461 tcactgcgaa atcactaaca tcgaacattc ggatatcacc tacttacccg ccctctggcg
     5521 caacccgcaa aaattactca aacacaaccg cgcactcctg ccttggctgc aggaacaaca
     5581 caacaggggc aatattattg cgggcgtagg cacggggtgt tgttttatgg cagaagcagg
     5641 ccttttgggc caccaaaccg ccaccaccca ctggcactat ttcgatgaat tcgcagccca
     5701 ctaccccaaa gttaaattac tgcgggatta ttttattacc cgtgcaggca acctctattg
     5761 caccggcagc gtaaattcca ctgcggattt aaccatccaa tttattaggc actattactc
     5821 agccgcagca gctaatcatg tggagcggca tttcttccat gaaatccgca acagcgggct
     5881 ccatgtcggc agcccccagt gggaccaacc ccatcgcgat gagcttattg cccaagcaca
     5941 agcctttttg ggagagcatt tagaaagcgc cctgcgcatg gaacaggtcg cccagcattt
     6001 tggcttgagt ttgcgcagct ttaaccggcg ctttaaagca gccacagctc aaacgccttt
     6061 aaattattta caggagcgta ggattttatt ggccaaggaa ttgcttaaaa ccagcaacct
     6121 atcaatcgga gaaattgccc tgcgctgcgg acaccaagat tttgcccatt tcacagggct
     6181 attcaaaaag aacctgggca ccacacccag ccaataccgc acaacagtgc gtgcaaaatt
     6241 atttgccgtt gcctaaattc acagggtaaa tctacaaatc gataactcgg cattgcgggc
     6301 cacaggcaaa aacagggaat agcgaaaagt tcctccttaa ctgcacattc agtgtgctcc
     6361 actcctcaca acaagcacgg tgacggctta gcttaattca tgccaaccga tctcataaac
     6421 tccttgaaat acctagtttt tttcaccatc cgcgcttaat accaaaaaag aataagcgta
     6481 gaaagaaacg attttaaaaa atatgaaaaa caggttgaat ttttatttca aaagtccatg
     6541 atgagtatgc ggcccgcgaa aaaaccaatt aaaaaccacc ataccgtgaa tggaggtaat
     6601 tcatgaagtc atccgcagat gttgtttatc gtgattttga ttcgtcccct gcattgacca
     6661 gcattatcga aaaacgcctt gaaaagttgg gtcgtttttc agattcgatt atgagtacac
     6721 gtgtagtact cgaaagtccc cacaaccaca aacacaaagg caagatgttc cgagcctgca
     6781 ttgagatggc cgtaaaaggc agcccggtta ccgtaactca cgatgatcca tctgtgcatg
     6841 tagcagttcg cgacgccttc caggcagccg aacgtaaaat taaagaagta gaggagcgta
     6901 gacgcgttag ccactaaaac taaatcaggc gcaagccaat tttagtagtc gctaaatttt
     6961 atatctctca gctcttaatt gagcccactc ggctgatatc ctcagccata aaagcccgct
     7021 gatgcgggct ttttattgtc tgaaaaaatc gatcgtcaca tttgggacta agaacctgta
     7081 acaacgattt ttaaaaattt accacatcag aaattaccta cacccagctc aattgggtta
     7141 tacctaggcc acaaaattta ccttaaaaaa tacttatttt ttccgctcat tggttggcac
     7201 actgcaaacc aagtggctcc aacagatgct tttaccttca attgtgcgta acatgccgac
     7261 cctttttaaa tcattacagg ggtattgtcg tgtttattgg cttagacttt ggaacctccc
     7321 agtgcgccgt gacggctgca accccaaatg gcttggtttc agtccctctg gaagctcagc
     7381 agccttttct cccgtcctgt atatatacgc ctgaacgctg tttaattagc gattttgtgg
     7441 cagctgcgat tagccaggag aaacacgagt acatgcaatc gcgccatacc ccactacaac
     7501 tggtacccga aaccaaacga aacctctcca tccggcacag ccgcgaagca ctgttattcg
     7561 gcacagatgc gatggcgcag cacatggcgg atccaatcga tggattcttc ataaaatccc
     7621 ccaaatcttt tctaggcgcc aacggcctac gcgaagatat ggtgagcttt tttgaggacg
     7681 tggttaccgc tatgctccta gaaataaaac agcgcgccga agcctattta caacacaata
     7741 ttacaggtgc cgtgataggt cgcccggtta actttcaggg cataaatgcc gaagccagca
     7801 accgccaagc acaaggaatt ttgtctaccg cagccctgcg tgcagggttc actcaagtag
     7861 agtttttgta cgaaccctta gccgccggtc tcgatttcga gcgcaagtta accgaagaca
     7921 aaaccgtgct ggtaatcgac attggcggcg gcactagtga ttgctcgatg gtgcgtatgg
     7981 ggccaaatca ccggcaaaag gtgaatagac agcgagactt tctagcccat acgggtgagc
     8041 gaataggggg caacgacttt gatataagcc tagctcgaga agcattgatg ccttattacg
     8101 gcatgaacag cccgctaaaa aataatttac ctatgccagt attgccattt tttcaagcta
     8161 ccgcagtgaa tgacgttgcg gcacaaagtg ctttttatag cgacaaaatg cgactgatac
     8221 tagagcaact aagccgcgat acaatcgagc cagatgtttt tcaacgattt atacacttgc
     8281 gagaaaacaa gcaaaatttt gcattaattc gcaaagcgga attagccaaa atcgccctta
     8341 gccataacaa taattcagag gttgatttaa cggcatacac aacaggttta atcgtagata
     8401 tccaacacca acaactagtt gcagccagtc aaattttgtt agacaaagtt aaacacctag
     8461 ctcaggaggc catcaaccag gcgcaacaaa tacccgattt ggtttattta acaggtggca
     8521 gcgccaagtc tagattatta aaggagactc tcgcacagta tctgggcacg cagatgcctt
     8581 taatagaagg ggatcacttt ggcagtgttg tatcaggact tggggtttgg gcggggaaac
     8641 tggcaaccta atctgaattg gctaaaccga ggaaacctcg tagattcaac ttcgaagcaa
     8701 atcaatgctg gtctaattaa atgatatttt aaaataagcg acagatgaga tttagctcaa
     8761 tcgtaaattt actaactgtc acggcttgga aaactccacc taatcacaac ccctctgaga
     8821 cctggaagga attaacacgg agcaagttat ttagctgcaa caccacagat ttattcaaca
     8881 aataattttt taacttgtcg gataataaaa taatgcccaa aaaataaaaa gccagtttga
     8941 cttccaaact ggcttttcaa aacctgacat accaaaagta tcaggctatt taacgtctat
     9001 cgtccagtta tagttgtaaa tataagcggc cgtatctttg ctgccaacat aaggcggtac
     9061 ttcaatcttc atggttaaca ctacgacacc aggagtcgta ggcgcggtaa ataccgcgta
     9121 gtaagaaccg tttggctgtc tctcgtaggg agtcactgtc aaaggatctt ttggacttag
     9181 caaagtcgca ccggaggaga cagcgaatgt taccttggtt ccgcctggca gagagttacc
     9241 cgttgcacat gctcccgaga gattcacggc gaatggatta ccgtctgaga gatataaccc
     9301 agacaaactc aaagtaccgc caggtgcaac tgtaagaggt acactaccat ttccaaaggt
     9361 tcccgcctgg caaatggttg gccccgaata ccctgacaaa ttaactacag cagtatcttg
     9421 aatatcaacc aatctttttg cagccccaca caaagatgag tgagcacagt ttaagccatt
     9481 ccattttcca tcaggcccat ctcgaacacc atttacgaca gaatcaacaa agtactcgcc
     9541 aagctgatag ctgccatcgt cattttcatc cacatacggc tcgtacgtgt catcagaatt
     9601 caagtcaaat atatcgttat cgtcgaatat aagattgcca ttcacatcaa tgaaatgctc
     9661 aaccccttta acataggcgg cgattcgcaa gcgtccatct tttggctgaa tgccagttga
     9721 ttgaaagcct acggcacacc gacctttcac tgtcaaacag gttggagtaa ttagcccacc
     9781 ctctggactt acaaaattca ccagcgtccc atcaataact gggttccctg atgcatccgc
     9841 aacggtcgcg ctaacggtga cagcatcaat tccagaatca aaattgtaag catgtgggtt
     9901 aaaactagag agcgcaatcg agaaccgccc tggcgcagaa agccctgacg caactgccaa
     9961 accaagtgag ctagcttttg caccattgga ttcaactgtt gccaacactg taacgttatt
    10021 gggcgtagtt ccaggtttta cttgagtttt aaccaagcca ttaagatctg aaacggcagc
    10081 cagggtaact aactctacac caccggcttc accaacgatt gagaatctaa cgttttcgcc
    10141 agctacgccg ttgccagatt gcccgagaac cttgaatgtt acagtcgcga aaactccgct
    10201 acctgaacca gtagtcgaga taggtatttg tgttgggctt gcacttacaa aggtgatacc
    10261 gcctacggcc tcaccagccg cggagaactt gtagctgccg acagttactg tttttccgcc
    10321 gccttgagca ttaaacgtaa ctacatcgtc gccaacacac ccttttgcgg tataggtgaa
    10381 cttaacagta ttcccagaaa cattcggagc cgatatctgc gaccgacttg agacgataca
    10441 agcagatgtt gcgctgaacg cactggtcaa ggtttttggg agagattgct cgtccttaaa
    10501 ggtaagggtc acctctttag aggtatcgaa catcacttgg ccagctggcg cagcgatgtc
    10561 gacccaaact ttctgattca gggttgggtc tatgaatgtt ccagcggtgt tcgcatctgg
    10621 aatgttatta gaatcgtccg gaggcgtatc gcttacaaca atcggccagg tcctagaacc
    10681 ttcgagcatt ccagggattt caatcttcat tgtcagaaac tgcggcccag cttgatcagg
    10741 tgcctcatag ataacaccca gtggaccctt tggaagtccc tcatccgctc cgactgtaaa
    10801 tactccactc ccccccttca aagaaccgcc gctcggagta aatgtaattt ttgtaccatt
    10861 gggaagtgga ttacccgttt tacacgggtg cccctcattt gaagctgatc catttccgtc
    10921 cgaaataaac atgccaccaa cggtcgttac accctttggc ttaacccaaa tggttttagc
    10981 aaaatcacct ggctcacaaa tctcgaacgc cccgtaactt aaaaataaag ttagttgctt
    11041 gctcaagtta accaattgag aagcaggtgc acagagcgag ctgtgtttac aattaacgcc
    11101 attccacttc ccatcagcga catctctttt gccattgttg ttggcgtcgg caaaaaattc
    11161 tcctatatca tactgaccat tttgattgtc atcagaaaaa ggctccggca aatcaacaaa
    11221 ggtatcaccg tcgtcaaaaa cattattgct gttgttatcc ttgaactctt ccacgccctg
    11281 cacataagcc attacttgaa ctcgcccatc taaaggttgc tttttagagg gaatccaggt
    11341 gctggtacag gtaccgttta cagtcttaca actctctatt gaaccgcctt cgcgagtttc
    11401 aaagcgcaca gtggtgccat caggcacata gccaccggca gagtcagaaa cacgagcaac
    11461 gatttgtgtc gtttcatcat tgatacggcc ataagatcga atgttgtatt tcgttgcgcc
    11521 aatcgcaaag gtagattcaa ctggcatggc agctgacact tccaggccac cagaaaatcc
    11581 ttcaactttt gatttctcat gtaaggcctt aatggaaact gttgtgggaa cagtaccggc
    11641 cttgactcga acctccacaa gaccgtcttt atcagagtta tcggaagagg ttattaatgt
    11701 aatgccaccc gaagttccgg aattactaaa aactacaggc tcattttcca gaggcttgcc
    11761 accttgacct aatactttaa aagtcaatac ggactcttta ggtgtagagt cgccggcgac
    11821 ataaatttta ctgggatcag ctttaataaa ttggattgaa gccacagatt cgccaatggt
    11881 aaccattttg acggaaccta attttgcaac tgcagtgcct gttccagagg taaagtaaac
    11941 ccaatcatct cctcgacagc cctgattggt atagctgaaa gtcatgacat tagagtttaa
    12001 tgtaggcgtt gaaatagtcg cgcccttcaa agtggtaatg caattcgaac tagcttcaac
    12061 ggaaatacct tttggctcaa atacttttcc cgccgaatct ttaaaagtga gcgtaatctt
    12121 cttgctgacg ccaaacgcaa cctctccttc ctcgctggca acggtaacgt ttacagtgct
    12181 accagtttcg gagtctgtgt aggaacccac ggtcgagcca ccggaactag tactcgaagc
    12241 tgcgccagaa ctagaactgg acgtcaccgg aggtttgggc acaggctcac ccaacaatcc
    12301 ctctcctttt ttgcccccac taccacatcc agccaaacca aggctaaacg ccagtgcaag
    12361 caggggtaaa aatcggaaat ttggtttttt tagattttta taagacatag ttaatcccag
    12421 tagttatgtc gatccagatt ggatactcgg gtcggcactt gcggcaaggt gtgaatcagg
    12481 gtttagcctc actacctgtg ccagccgaac ggcaaggcaa gggtcaatga agcataatca
    12541 ccctaaccac gcaacgcaag gcgagctggc catcttagac ttttttctta atacaaatct
    12601 cagtttacta gtgcgaaaca gttaagctac gctcattgac acccttcgct tggaacgctc
    12661 tatgtacaat ttaacccccc atgaagcccg agtgatcggc agcctcatcg aaaaagccct
    12721 gaccacacct gatcaatacc ccctctcgct caacagcctc accatggcgt gcaatcagaa
    12781 gactaaccgt gagccggtta tggaattgcc ggaggcccag gtgcagagca ccttggatga
    12841 tcttgtaaag aagcacttgg tatcacctat ttcggccatg ggtagccggg tggtgaagta
    12901 tcagcaccgc ttttgtaaca cgcagtttag tgaactgcag ttatcgccag gagagttggc
    12961 cgtggtgtgt gtattgttgt tacgcggctg gcagacgccc ggtgagctgc gcacccgcac
    13021 tcagcgcctg cacgatttta atgatgtatt ggatgtggag aaggtgctgc accatttgct
    13081 ggaacgcagc cagccgctgg tatgcaggtt ggaacgggaa ccgggcaaac gggagcaccg
    13141 ttatgcgcat ctattgggta gcgatattca tgagcaggcc caatcaagta ctgctgtacc
    13201 acctgcagcg gagcgcaagc aggatgaatt aaccgcgctg aaagaggaat tggcacagct
    13261 gcgagaagaa tatgtgaagc tcagtgagcg ggtgagtgtg cttgaggggt tgttttagcc
    13321 ctctaacata cctaataaca ggctataaag ctgaatcaga gatttagaac ccctccccgg
    13381 ccctcccctt tttaagggga gggagaagag cagctgagat ataacacgcc taaggagtag
    13441 gcgctgggat ttacctgcag gcagcaaagc gctgccaggc ggcctggtag gcctcatcag
    13501 caacagcggg cgtggcgagc cactccaata ccaaagcctc catagtttcg tctcgcttgc
    13561 caaaatttag ctgcagttgc tccacttgat aggccatacg ctgggctttg aaggcttccg
    13621 gggtgtcttt ttggctgaga atttcggcgc ggatacaaag taaaagcaat tgattttcgg
    13681 catcttggct tttctgccgc aaagcgtcta aacctagctg tttacgttgg gacagaacgt
    13741 cttgagttcc tgcaggccac gagtttatgg ctgcccaggc cgcttcaagt tctggcgaat
    13801 caccgcaact atcgtcgctg gccaagcgat tgcactcatg gcggcgcaac aggtctgcaa
    13861 gattaaatac atcattccag gccttagcct gtgcgaagct gcgggcctgc tggagcgcct
    13921 gtttaatttg ctctgagtaa tggtggtatt ggctggttaa ttccgcagcc gctttgcgcg
    13981 gtaattcccc taagccttca aattgagttt ttagctcatc tatatgagtt ttggcactgg
    14041 caaccgcatc gggggccgac tccacccagg tttttaattg gtttaaataa tccttggctt
    14101 tttccaccac tgcatcgcgc tgggcgttaa cctcatcata aagctgtttg cgtttttcaa
    14161 acacggcgtc gcaatgcttg cgaaaatcat cccacagggt ttgatcgtct ttcggccagc
    14221 tgcggccgat ggatttccac tgctgttgca gctgttttac tttttccgca gcggcggcta
    14281 agtccacggc atctttaagt tcagccgctt cggcgattat tttttctttc caatttttat
    14341 tggcttggta ttcggttttg attttgccgt gtaattgatc caataaggcg tcaaattgtg
    14401 cctgctgctc ttttaccaat ttgcgcggta ctggccagta ggttttccac tcttcccgcg
    14461 ccagcttcaa ggttttttca acgtctttcc acacggcgtt ttgccaatcg tattgctcta
    14521 aataggcctg caattgagtg attaaaccgg tgcgtttggc agaattgctt tcgcgctctt
    14581 gggtttgccg ctcaaagtat tctttgcagg gcgtgtaggc ttgcacggag gccgcctgga
    14641 attgctccca aaggctctca tcattttgtt ggccgccgcg gcttaattct ttccattgat
    14701 cctgcaattc gtgaatttta ctggccaaat cattgggcgc cagtttgctc tcgctcagcg
    14761 cctgcatctg cttaattaaa tcttcttttt tcggtgtaac ggcaaactcg tgccagtcgc
    14821 cgagcttttg catcaggtgc tcaaagtcct gcaatttgcc ggtgatatta gcgggtaatt
    14881 cttcagccgc ctgctggacc ttttcttgta catctttata aatgccgcgg gctttgcgca
    14941 ccatgccctg ctcggctgcc cacacacctt tgcgggttaa atcagctaag tctttaacca
    15001 actggcgggc ttggtcttct ttatcttttt gcgtatcaag ccaggcgtta aattgttgtt
    15061 tggtttctat aagccaaggc gttacttgtt cgtgttgaaa atcttttgct tcttgcaaaa
    15121 tagcctgtac atggcgctta agattgtgcg cggtttctga attgcccgcc tcctgcagct
    15181 ggttgagtac ctgcactacc gggccggact cggatagttt attaaagagg ttgtgggcgc
    15241 gctctttgta atcgttaaag agtttcacct gctcggccgc gtggatattg cgcgccgcca
    15301 aactaattgc ctgcgtgagt tcctgaattt ttttcggtac gtccatggct ttaagttcat
    15361 ccatgtgtgt ggcggtgtag agtttggcca ataaattctg catatcattg acagctgcat
    15421 ctacaaattg ccgcgcctgc tgctctaggg caattttctc ttcttccttg gcaataactt
    15481 ccgctcggct ggctatt
//
LOCUS       DAOQGS010000017        15329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1780_length_15329_cov_5.054995, whole genome shotgun sequence.
ACCESSION   DAOQGS010000017 DAOQGS010000000
VERSION     DAOQGS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1780_length_15329_cov_5.054995"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKZ52_01270"
     CDS             <1..896
                     /locus_tag="PKZ52_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNG58424.1"
                     /translation="YLLREASKIKRYEFMLTNSALYSIAMMARVLLVSRSGYYSWLDN
                     REIVSWRMQQRESIDALVKAAFEAGKGRYGAERIFHDLAEQDKPLDIKTIRKSLKRQG
                     LIAKAAKLFKVTTDSNHSLPVAPNLLERDFSAQQPNEKWVTDITYIQTTEGWLYLAVM
                     IDLYSRKIVGWSMSKHIDAQLVCDALIMALWRRKFPKDVIVHSDRGGQYASHAFRDLL
                     EKYSLRQSMSRKGDCWDNACAESFFHSLKVELIHGEPLQDGMQTREFVFEYIEVDYNR
                     YRRHSAIGFISPERFEARTVC"
     gene            1654..2073
                     /locus_tag="PKZ52_01275"
     CDS             1654..2073
                     /locus_tag="PKZ52_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG58425.1"
                     /translation="MNIEIHKNPSLVDIDFIKEGLKGFNKDFGTEDFMNPMAVFVKDE
                     FGNPSGGAMFCIQYTSCYIHLFWIEAGARGRGLGRAVLAAITQECKSYGVKDMFLDTF
                     SFQNTGFYERLGFKSVGCLYNYPKQGINKYFYHAVLI"
     gene            complement(2310..4139)
                     /gene="glmS"
                     /locus_tag="PKZ52_01280"
     CDS             complement(2310..4139)
                     /gene="glmS"
                     /locus_tag="PKZ52_01280"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNG58426.1"
                     /translation="MCGIVGAVSERNVANILLEGLKRLEYRGYDSAGLAIINEKHELK
                     IAKSAGKVSQLVSVVDETPMPGHLGIAHTRWATHGVPNDTNAHPHRSGDICVVHNGII
                     ENYKELRAELQQQGYKFNSETDTEVIVHLVHLNHQKCGSILEALQLTIPRLHGAYGVA
                     VMCAAEPNTLLVTRSGSPLVIGLGIDENFIASDQLALRQVTDRFIYLEEGDIAQITIK
                     HVHIYDKDLEPVERKITTITEAADAAEKGPYRHFMLKEIYEQPAAIKRTLEGRLSKTQ
                     ALEEAFGVAAKAIFDQVECVQIVACGTSYHSGMVAKYALEDWAGIPVYVDIASEFRYR
                     KVIVPKNALFVTISQSGETADTLAALRLAKASGYLGSLAICNVANSSLVRESDLCLMT
                     NAGPEIGVASTKAFTTQLVALQILGLVLGRRHGLTQEHEAKLVAALHELPRLSEQVLS
                     LNPVIEQVSLAFVEKHHALFLGRGIQYPVAMEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDSDMPVVAVAPHNDLLEKLKSNLEEVQARGGKLFVFADHDAGFTADANTTVINIP
                     HVPDSLEPIIYTLPLQLLSYHVATLRGTDVDQPRNLAKSVTVE"
     gene            complement(4170..4940)
                     /locus_tag="PKZ52_01285"
     CDS             complement(4170..4940)
                     /locus_tag="PKZ52_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNG58427.1"
                     /translation="MNKRNTQQRRRAIIELLNQTGEVTVEQLAQRFTTSEVTIRKDLA
                     ALEANGLLLRRYGGAMLLPQELMADPAVEKVSNRKLAIAKAAAELIRDHNRIIIDSGS
                     TTTALLLPELSQKQGLVVMTNSLSIANALRELEQEPTILMTGGTWDPKSEGFQGQLAE
                     QMLRSYNFDQFFIGADGLDLARGTTTFNELFSLSRVMAEVAREVVVLAESNKIGRKIH
                     NLELPWSMVHVLVTDNALTMEAKQSIEQQGVRVITATV"
     gene            5228..6388
                     /locus_tag="PKZ52_01290"
     CDS             5228..6388
                     /locus_tag="PKZ52_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNG58428.1"
                     /translation="MPTFIHQELALPNPAFNSPLVDVLTELEHLRRLQLTGTTPPPLF
                     FQLKHIFHMLESLGSARIEGNHTTLADYIESKLDGSAEHTDQLREMENIERAMEHVEQ
                     TISQGTVLAEHFIRELHAIAVEDLVREGDATPGAYRQGSVRISQSQHLPPDAFLVPQY
                     MQELAAFINEQHAPKYDLIKVALAHHRFGWIHPFGNGNGRVVRLLTYALLIKYGFNVQ
                     AGGRVLNPTAVFCNDRDKYYAMLAQADSGGKDDLEAWCTYVLEGILRELKKVDRLTDY
                     KYLRDKILAPAFTYARERKFITAQEESILRIAAKSGRTKAADLSEAMPQMSAAQRTYQ
                     IKKLVERKMLQPVSEGARQYTLGFSNNYLIRGVVKALSKEGFISATLDGEKS"
     gene            complement(6533..8392)
                     /locus_tag="PKZ52_01295"
     CDS             complement(6533..8392)
                     /locus_tag="PKZ52_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG58429.1"
                     /translation="MIRLFETRIKGTAEFAPATPPAKLAAFYWHYVRQTKWCYAAMVF
                     SVLLVALMDLLIPVMIGRLTEFLQGADRLAAITAQWPQLVAMAAILLLLRPMVQLFDV
                     AVRQNLLLPGTTSLVRWQSHWHVVRQSWPFFQNDFAGRIANRIMQTPNALRESIMSSI
                     RAGLFITTYGLSSLALMAWADLRLALPTFVWIGAYITFLRYFVPRLLALARISSEARS
                     TTMARVVDSYTNILTVKLFARAADEDQHVREAVDHHQDAMAKHMRVMTYYLWTLNILN
                     ALLITGTAAVGIFLWSQNMISAALVATAVPLAWQIANMSGWISFEVAGIFENIGTVQD
                     GMQSIAVPHTLIDAPEAKPLQISRGEINFAQVNFAYTPGRPVLQDFNLHVQAGERVGV
                     VGRSGAGKSTLINLLLRFHDINSGAILLDGQDIRSVTQESLRSAIGLVTQDTSLLHRS
                     IAANIRYGHPEATDDEVRAAAIKAQAHEFISNLVDWDGRTGFNAHVGERGVKLSGGQR
                     QRIAIARVILKNAPILILDEATSALDSEVEIAIQEQLLGLMDGKTVIAIAHRLSTIAR
                     MDRLIVLDQGRVVEQGTHAQLLAANGHYAALWQHQSGGFLPEQLPEDVWVQEA"
     gene            complement(8617..11715)
                     /locus_tag="PKZ52_01300"
     CDS             complement(8617..11715)
                     /locus_tag="PKZ52_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNG58430.1"
                     /translation="MIGWIIRTCLHNRVLVLLITLLLCLLGGRALHTTAVDAIPDLSD
                     VQVIIKTSYPGQAPNIVESQITRPLTSALLSVPGAHTVRGFSFFGDSFIYVIFKDGTD
                     TYWARSRVLDNLNQAAKKLPPSASAEIGPDASGVGWVYIYALHSKNNQQDLAQLRALQ
                     DNFLRYELQTLPGVAEVAPIGGMVKEYHIQPDLNKLMLYGIPLMHIKNAVTHNNRETG
                     LAAIELAEAETMLRATGTITSAASLGQTPLGIMDKNGAPLLLNQVAEIIEAPAMRRGL
                     ADLNGRGEVVGGIIVMRQGENAQKTIAAVKEKIAQITPALPAGVEVTPVYDRSQLISR
                     AVNNLWHKLAQELILLGILLLVFLLSVRSVFAALITLPIGIVLSFLLMHWQGINANIM
                     SLGGIAIALGAMVDGAIVMVENLQRRIATQKTAADHLQSVYDSAMEVGPTLFFSLLIV
                     TISFMPIFTLDGEEGRLFRPLALTKTYAMASAALIAITLMPVVLYYLSGKLPAGHVHA
                     ARLHTHLLILYQRLLKRCLVKPTPFLLCVGFIGVAALMPLKFLASEFMPPLDEGDLMY
                     MPTSAPGISIAKARELVQQTNKIISALPEVERVFGKAGRAETATDPAPISMLETFIQL
                     KPRSQWRSGKTTADLIAELNSSIQFPGVSNAWVMPIKTRLDMLATGLKTPLGIKIAGP
                     DINTLEQLGSQIESLLKELPQTRAAYSERSLGGRYLTLDINKQAAAAFGFSLDDIHEW
                     LQMSLGAMPIGETIEGAERYAITLRFAEDYRSTPERIENLPIMGKNGVLIRLMDVAQV
                     RLESGAHMLKSENGRLNNWIYIDIGDADVNRYVSDFNQRLQQQITWPTGYSLRWAGQY
                     ESMQRVRASLAFIIPLTLGIIALLLFVNFRSLCDVALLLASLPFALGGGLILVAALEF
                     KFSVAVAVGFIALAGLSIATSALMLQVLRAQIPAQLNCDINSLHKQIIDTASLRLRPV
                     LMTATAMMAGLMPVMFGSEPGSEVMERIAAPVIGGLFSSVLVTLFVLPVVYFRLQLKK
                     AN"
     gene            complement(11712..13175)
                     /locus_tag="PKZ52_01305"
     CDS             complement(11712..13175)
                     /locus_tag="PKZ52_01305"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG58431.1"
                     /translation="MSKTPPAVIAPLRRYRFSLLGIMALLVASIAWFAPWQATAPKPL
                     YWVSSMDPNYRSDKPGKCPHGMDLIPVYAEDAKATEQAGRVKINPAVQQQMAVRKVKV
                     ARGVLNQRLQSFGRVVADPSLVSPISPRSQGWVDMLFVSGEGEAVKKGQPLFSFYSPD
                     LIEAQERYLAARRQGAAQAMKAEEDLKDLQMDSISLARLKDQGLAQRSVIFHAPKDGV
                     VDMLKLREKAYFSPGQMVMAIGSMERVWVWLEVFASQGPLIAPRQAVSLTSQSLPGKV
                     WEGEVDAIAPDLDPKSRSLRLRVSLANPNMQLRPNMLMQGLISLPPRPPALLVSRQAV
                     IELDNQARLVMEAPEGTFKSVGVTLGARNEELVEVLAGVEEGDIVVTSAQFLIDAESS
                     KSSDLKRLQAPEVAPAYPPTWVKARISQLFPNERKIQLEHEAITAWQMPGMTMNFALT
                     DAINIEHFKLGDQLNVKITDGSPLFQIIDVKAADESP"
     gene            complement(13204..14607)
                     /locus_tag="PKZ52_01310"
     CDS             complement(13204..14607)
                     /locus_tag="PKZ52_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNG58432.1"
                     /translation="MAYLPPLLSALGRKRLGGLVIAAANLWAAHQVAAAQGAAIPPQS
                     AQQQEVDTARQELIKLEAALNQAVQAAERSNVADLAELKQRQALAERFVFRTSSRAEL
                     DQGKPAQTAQRLAGYRRLIEQYNLIEPSIAAEKRAELTRTDSKMYYQMRVRSALPPKA
                     LKAYGLMELARQERDQGKFKEALARWEQAETLVRESFNDHIAAMAKWRQDALKDAAVQ
                     EEKTRLQVEAWLKTAMVAIPGGGFLMGGSPESSDETPAHQVQVPAFSLSKTEVSFALY
                     DLCVASSSCYYVPPDEGWGRGNRPVINVSYQDINQQFLPWLNKLTGRSYRLPSEAEWE
                     YAARAGTSSEYWWGQNASCDQARFDGGAASPCWVKEGKNRGSLPVQSFKANGFGLFDV
                     HGNVWEWVEDCWHPNYQGAPTDGSPWLADNCAIRVLRGGAWDYPASGMRAANRYYFSH
                     TSRKSSYGFRLAISAKP"
     gene            14971..>15329
                     /locus_tag="PKZ52_01315"
     CDS             14971..>15329
                     /locus_tag="PKZ52_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNG58433.1"
                     /translation="MSDHTRPQPFCWRAHSISHPGSVRAANEDAVLCRDDVQLWAVAD
                     GMGGHNRGDLASNTLVRHLERTDASPSLADAVDALEDSLLNANRAVLNYAQTHFHGAI
                     MGSTFVAVLIRGNLGFVA"
BASE COUNT         3630 a         3842 c         4067 g         3790 t
ORIGIN      
        1 gctacctact tcgcgaagca tcaaaaataa aacgctacga atttatgctg acaaatagcg
       61 cgctatattc aatcgcgatg atggcgcgcg ttctgttggt ttcgcgaagt gggtattaca
      121 gctggcttga taatcgtgaa atagttagtt ggcgcatgca gcaaagagaa tcgattgacg
      181 cgttggtaaa ggctgcattt gaagctggta aaggtcggta tggtgccgaa cgaatttttc
      241 atgacttggc ggagcaagat aaaccgctcg atataaaaac gattcgaaaa agcctgaaaa
      301 gacagggatt aattgcaaag gctgccaagt tattcaaggt gacgactgac agcaatcatt
      361 cgctgccagt tgcgccaaac ctgttggaaa gagatttttc tgcgcagcaa cctaatgaaa
      421 aatgggtaac ggatattacc tacattcaaa caactgaagg ctggctgtat ttagcggtga
      481 tgattgattt atattcacgt aaaattgttg gttggtcgat gagtaaacat attgatgcgc
      541 aattggtttg cgatgcgttg atcatggcat tgtggcgacg caaatttccc aaagacgtta
      601 ttgttcacag cgaccgaggg ggccaatacg catcgcacgc gtttagggat ttactggaaa
      661 aatattcact caggcagagt atgagtcgca agggcgattg ctgggataat gcgtgtgcgg
      721 aaagcttttt ccactctctt aaagttgagc tgattcacgg cgaaccgtta caggatggaa
      781 tgcaaacacg cgaatttgtt tttgaatata tcgaagtaga ttacaatcgg taccgccgcc
      841 acagcgcaat tggctttatt agccctgagc ggtttgaagc aagaactgta tgttagttaa
      901 ctgtccgata ttggcggtag gggtcaaagg aatcatttgt tctgatttta aaaaatggtg
      961 gcagaaatgg tggcaggaaa gccttatttg ggtaattaaa attacataat tcattgattt
     1021 atatttaatt tatttaattc acaccacaaa caggtgcaga atattgaata aaaaaccgcg
     1081 ccgctaggga tgccgctgaa caaaccttaa ttcgcagttg attcgcaacc gtatatagag
     1141 ggatctggac gcggcgattt cccgccagcc acctatagga tcccatgtct ttgcagctcg
     1201 cgggcggaaa ataatcactc gtccaatagt gcctagcagc cctttgctgt cctatggaat
     1261 gatgtcggtt acattaagta aattatgcaa tagcttctag cagcttgttg aaatacttag
     1321 gcggcgaccc actggcaagg caaatttggc gaaaaaaccg aacgactgca tggatgcagg
     1381 aggtagagca atgcaggagc aattgccgag tttacaccca gtaaatgagg attttttcgc
     1441 caaatttaac gccgccaacg ctcgcaaggt agtattcaac aagctgctag agtatatgaa
     1501 tgggcaatcg gggttaagtg gggtttgttg gtcgcttaga tcccattacc taaccatcgc
     1561 gattttttga cgcacatcat cgttaagaaa ccaaacgata cgccgtcctt atctgttgcc
     1621 tattaattgg gcatttactt gaagcgagcc gatatgaata tcgaaatcca caaaaaccct
     1681 agcctagttg atattgattt tataaaagaa gggcttaaag gttttaacaa agactttggc
     1741 actgaagatt ttatgaatcc tatggctgtc ttcgttaaag atgagttcgg caatccatcg
     1801 ggcggtgcga tgttttgtat tcaatatacc agctgttata tccacctatt ctggattgaa
     1861 gcaggtgcac ggggtagagg attgggccgg gcggtgttag cggccataac ccaagagtgc
     1921 aagagctatg gagttaagga tatgttccta gatacgtttt cgtttcaaaa tacgggattt
     1981 tatgaacgtc taggatttaa gtcagttggc tgcctataca actacccgaa gcaaggcatc
     2041 aataaatatt tttatcatgc tgttcttatc taactgagct attaatttag tcgccgagag
     2101 tgcaagattt gtggcagcgt taaagaccag ggcgcgcagg attggctaaa tgcaggttgg
     2161 tggtgagcct gtgcccccaa cattccttcc aaactggaaa aagaacccaa tctaaacaag
     2221 ataaaattga tcgcaactct aagcttgttg agggctggaa tgttggggtt tgtgaactcc
     2281 cccccaaccc attaaaacct aaaaaacaat cattctacgg taacgctttt tgctaaatta
     2341 cgcggctgat ccacatcggt gccgcgcaaa gtcgccacat ggtaactgag caactgcagc
     2401 ggcagcgtat aaataatcgg ctctaaacta tcgggcacgt ggggaatatt aattaccgta
     2461 gtattggcat ctgcggtaaa acccgcatcg tggtcggcaa atacaaataa tttaccgccc
     2521 cgcgcctgca cttcttctaa gttggatttt aatttttcca acaaatcatt atgtggcgca
     2581 accgctacta ctggcatgtc tgaatccacc agcgctaatg gcccgtgttt taattcccca
     2641 gcggggtagg cttcggcgtg gatgtaggag atttctttta attttaaggc accttccata
     2701 gccacagggt attgaatacc ccggcctaaa aataaggcgt ggtgtttttc cacaaaggcc
     2761 aggcttactt gttcgatcac cgggtttaaa gataatacct gttcgctcaa gcgtggtaat
     2821 tcgtgcagag cggcgactaa tttcgcttcg tgttcttgag ttaaaccgtg gcggcggcct
     2881 aaaactaaac cgagaatttg cagggcaact agttgtgttg taaatgcttt agtagaagca
     2941 acgccaattt ctgggcctgc gttggtcatt aaacacaggt ccgattcgcg cactaaagag
     3001 ctattggcta cgttacaaat cgccaggctg cctaaatagc cgctggcttt agccaggcgc
     3061 aaggccgcta aggtatcggc agtttcaccg ctttgagaaa tagttacgaa taatgcgttt
     3121 ttcggcacta ttactttgcg gtagcgaaat tccgaagcta tatccacata taccggaatt
     3181 cccgcccagt cttctaaggc gtatttggcc accatgccgg agtggtaact ggtgccgcag
     3241 gcaacaattt gtacacattc cacttggtca aaaatggctt tggcggccac accaaaagct
     3301 tcttcaagag cttgggtttt gctcaggcgg ccttccagag tgcgtttaat ggcagcgggc
     3361 tgctcgtaaa tttctttgag cataaaatgc cgataggggc ctttttcggc ggcatcggcg
     3421 gcttcggtaa tggtggtaat tttgcgctcg acgggctcta aatctttatc gtaaatatgc
     3481 acgtgtttaa tggtgatttg ggcgatgtcg ccctcttcta aataaataaa gcggtcggtg
     3541 acttggcgca gggctaattg gtcggaggcg ataaaattct cgtctatacc caagccaatt
     3601 actaatgggc tgcccgagcg ggtaaccaat agggtatttg gctcggcggc gcacatcacg
     3661 gcaaccccat aggcgccgtg taaacgcgga atagttaatt gcagcgcctc taaaatagag
     3721 ccgcattttt ggtggtttag atgcactaaa tgcacaatta cttcggtgtc ggtttcggaa
     3781 ttaaatttat aaccctgctg ctgcagttcc gcgcgcaatt ctttgtagtt ttcgataata
     3841 ccgttgtgca ccacacaaat atcgccggag cggtgtgggt gagcgttggt atcgttgggc
     3901 acaccgtggg tggcccagcg ggtgtgggct atgcctaaat ggcccggcat gggggtttca
     3961 tccactacgc taactaattg ggaaaccttg cctgcggatt tggcaatttt taattcgtgt
     4021 ttttcgttaa tgatggccag gcccgccgaa tcatacccac gatattccag gcgttttagg
     4081 ccttctaata aaatgtttgc cacattgcgt tcgctcaccg cgccgactat gccacacata
     4141 agtaaatcct ctaattaaaa aataattaat taaaccgtgg cggtaattac ccgcacaccc
     4201 tgttgttcaa tactctgttt agcctccatg gtaagcgcgt tatccgttac taaaacgtgc
     4261 accatggacc aaggtaattc caaattatga attttgcgac ctattttgtt ggattcggcc
     4321 aacaccacta cctcgcgggc aacttctgcc atcactcggc tgaggctaaa taattcgtta
     4381 aaggttgtgg tgcctcgggc taagtccagg ccgtcagcgc cgataaaaaa ctggtcaaaa
     4441 ttgtaagagc gtagcatctg ttcggccaat tggccttgga agccctcgga ttttgggtcc
     4501 caggtgccgc cggtcattaa tatggtgggc tcctgctcca gttcccgcag ggcattagcg
     4561 atgcttaagg aattggtcat taccaccagg ccctgtttct gggataactc gggcaataaa
     4621 agcgccgtgg tggtgctgcc gctatcaatg atgatgcggt tgtggtcccg gatcagctcg
     4681 gcggcggcct ttgctatggc cagctttcgg tttgaaactt tctccaccgc tgggtctgcc
     4741 attagctcct gcggcagcaa catggcgccg ccatagcggc gcaggagcag gccattggct
     4801 tccagggcgg ctaaatcctt tcggatcgta acctctgagg tggtaaagcg ctgggccagc
     4861 tgctccaccg tcacttcccc ggtctggttt aacagctcaa taatagcgcg gcggcgctgt
     4921 tgggtgttgc gtttgttcat ggcggcggct attccggggt ggcggcgaag cttaacgcaa
     4981 aaagtttcga aatgaaagtt atgcctttgg ttttaggtgt cgggttgctt gcgggcttct
     5041 gggcaacctc caacagatgc aagaacgcaa gcttaacgac agagatgctt atttgagtct
     5101 attctattta ctgcaacagc cacttaaaga tatttacctg ccacataaat aataactaat
     5161 tgactttatt gatatttttt tattaaatta cagatgttat gatcttgccc tagagataac
     5221 tatcgatatg cctacattta ttcaccaaga gttggccctg cccaaccccg cgttcaactc
     5281 ccccttggtg gacgtactga cggagcttga acacctacgc cgtcttcagc ttaccggcac
     5341 aacaccgccg cctttgtttt ttcagctgaa gcatatcttc cacatgttgg agagcttggg
     5401 ttccgcacgc atagaaggca accacaccac cttagcggat tacattgaaa gcaagcttga
     5461 tggttcggct gaacatacgg accaactgcg agaaatggag aatattgagc gcgccatgga
     5521 acacgtggag caaactattt cccaaggcac agtgctagct gaacatttta tccgtgaatt
     5581 gcatgctatt gccgtggaag atttggtgcg tgaaggcgat gcaacaccgg gggcctaccg
     5641 ccaaggttct gtacgtattt cgcaatccca acacttgccg ccagacgcat tcttggtgcc
     5701 tcagtacatg caagaactgg ccgcttttat taatgagcag cacgcaccca aatacgattt
     5761 gattaaagtg gctttggcgc atcaccgttt tggttggatt catccatttg gtaatggaaa
     5821 tggccgcgtc gtacggttgt taacttatgc actgcttatt aagtatggat ttaatgtgca
     5881 ggctgggggc cgtgtactta atcccacagc tgtattttgc aatgatcgcg ataaatatta
     5941 cgccatgctc gcccaagctg attcaggcgg aaaagatgat ctagaagcat ggtgtactta
     6001 tgtattagaa ggcattttga gggaactaaa aaaagtggac cgcctgaccg attataagta
     6061 tttgcgcgat aaaattttgg ctcctgcctt cacctatgcg cgtgaacgga aatttataac
     6121 ggcgcaagaa gaaagtattt tacgcattgc agcaaaaagc ggtcgcacta aggcggctga
     6181 cttatctgaa gccatgccac aaatgtcggc tgcgcaacga acctatcaaa ttaaaaaatt
     6241 ggtagagcga aaaatgttgc agccagtaag cgaaggcgcg cgtcaatata ctcttgggtt
     6301 cagcaataat tatttaatac gcggcgttgt caaggcgctg tcaaaagaag gatttatttc
     6361 agctacatta gatggagaaa aatcataggg ggcgaagaaa aagtatcttc taagtagaag
     6421 agcttagtcc gtagaacacc ttggtacgcc ccttgtggtc ctcacgcgtt ttgagggcgg
     6481 aaagtccgat aggcaatccc acgcagagca tgggaacgag agtgaaagga aatcaagcct
     6541 cttgaaccca aacatcctcc ggcaactgct ccggcaaaaa cccacccgac tgatgttgcc
     6601 agagcgccgc ataatgacca tttgcagcga gtaattgggc gtgggtgcct tgttccacta
     6661 cgcggccttg gtccaacaca attaaacggt ccatgcgcgc aatggtggat aaacgatggg
     6721 ctatggcaat taccgttttg ccatccatta aacccaataa ttgttcttga atggctattt
     6781 ctacttcgct atctagcgcg gaggtggcct cgtctaaaat taaaataggc gcatttttta
     6841 atattacccg cgctatggcg atgcgctggc gttggccgcc ggagagtttt accccgcgct
     6901 cgcctacatg ggcattaaaa cctgtgcgcc catcccaatc caccaggttg gaaataaatt
     6961 catgggcctg ggcttttata gccgctgcgc gcacttcatc atcagttgcc tctgggtggc
     7021 cgtagcgaat attggcggcg atggagcggt gtaggagcga ggtatcctgt gttaccaggc
     7081 cgattgcact gcgcaaactc tcttgggtaa cgctgcgaat atcttgccca tccaacaaaa
     7141 tagcgccgct gttaatatcg tgaaagcgca gcaataaatt aattaaggtg gatttacccg
     7201 caccagagcg ccccaccaca cccacccgct cgcctgcttg cacgtgtaaa ttaaaatctt
     7261 gcaacacagg acgccctggc gtgtaggcaa aattcacctg cgcaaaatta atttccccac
     7321 gggatatttg cagcggttta gcttccgggg catcaattag cgtgtggggc acggcgatac
     7381 tttgcattcc gtcttgcacc gtgccgatat tttcaaaaat gcccgccact tcaaaagaaa
     7441 tccaaccgga catattggcg atttgccagg ccagcggcac ggcggtggcg accagcgcag
     7501 cgctaatcat gttctggctc cacaaaaata tgcctaccgc tgcggtgccg gtaattaata
     7561 aggcgttgag aatatttagc gtccacaggt aataggtcat cacccgcatg tgtttggcca
     7621 tggcatcctg gtggtggtcc accgcttcgc gcacatgctg gtcttcgtcg gcggcgcggg
     7681 caaataattt caccgtgaga atattcgtat aactgtccac tacccgcgcc atggtggtgg
     7741 agcgcgcttc ggaactgatg cgcgccaagg ccaacaagcg cggcacaaaa taacgcaaga
     7801 aagtgatgta agcgccgatc cagacaaaag ttggcagcgc caggcgcagg tccgcccagg
     7861 ccattaaggc taaggagctc agcccatagg tggtaataaa taaacccgca cgaatggacg
     7921 acataatact ttcgcgcagg gcattggggg tttgcatgat gcggttggcg atgcggccgg
     7981 caaaatcatt ttgaaaaaac ggccagcttt ggcgcaccac atgccagtgg gattgccagc
     8041 gcacaaggct tgtggtgccg ggtaataaca aattctgccg cacggctacg tcaaacagct
     8101 gcaccatggg ccgcagcaat agcaatatcg ccgccatggc caccaattgt ggccactggg
     8161 cggtaattgc agctaagcgg tcggcgccct gcaaaaattc agtgaggcga ccgatcatta
     8221 ccgggattaa caaatccatc agggccacca gcaacacact gaataccatg gcggcgtaac
     8281 accatttagt ctggcgtaca tagtgccaat agaacgcggc gagtttggca ggtggggtgg
     8341 cgggggcaaa ttcggcggtg cccttaatgc gggtttcgaa caatcggatc atggtggcgt
     8401 ggcggctaga tggagagtgc cgttagtgta gcgggtgtta gctgtagttt gcgcactcgg
     8461 atcgattgca catgatccca tgcacctgct ggaaattatc gggcgagtcc tttgctgatc
     8521 tccttcaata gttatggcgc ccgcagtgat tatcctgaca gcctgttgga ataccacctt
     8581 gcgagccttg acggcgttaa atctgtgcta gcgccgttaa ttcgcctttt ttaattgaag
     8641 cctaaaatac accaccggca atacaaacag cgttaccagc acactgctaa acaagccgcc
     8701 gataaccggc gcggcaattc gctccataac ttcgctgccc ggttcactgc caaacatgac
     8761 tggcatcagg cctgccatca tggcggtggc ggtcattaat accggccgca ggcgcaggct
     8821 tgcggtgtcg attatttgtt tgtgcaggct atttatgtcg caatttaatt gcgcgggaat
     8881 ctgggcgcgc agtacctgca gcattaaggc gctggtggcg atgctaagcc cggctaacgc
     8941 aataaaaccc accgctacag ccacggaaaa tttaaactct aaagcggcca ccaaaattaa
     9001 cccgccacct aaggcaaagg gtaggcttgc taataacagg gcaacatcac aaaggctgcg
     9061 gaaattaaca aataataaaa gggcaattat tcctagcgtg aggggaatta taaatgcaag
     9121 gcttgcgcgc acccgctgca tggattcgta ttgcccagcc cagcgcaggc tataacctgt
     9181 tggccaagta atctgctgtt gtaaacgctg attaaaatcg ctcacgtagc ggttgacatc
     9241 ggcatcgcct atgtcgatat aaatccagtt atttaaacgg ccattttcgc tttttaacat
     9301 atgggcgccg ctttctaagc gcacttgggc aacatccatt aaacgtatta atacaccgtt
     9361 tttacccata ataggcaggt tttcaatacg ctcgggggta ctgcgataat cctcggcaaa
     9421 acgcagggta atggcgtagc gctcagcgcc ttcgatggtt tccccaatgg gcatagcgcc
     9481 taggctcatt tgcagccatt cgtgaatatc atccaggcta aaaccaaaag ctgccgccgc
     9541 ctgtttattg atatccaatg tgaggtagcg cccacctaaa ctgcgttcgg aataagccgc
     9601 gcgggtttgc ggcaattctt ttaaaagaga ttctatttgg ctgcctaatt gctccagtgt
     9661 gttgatatcc gggcctgcaa ttttaatccc caagggggtt ttaagcccgg ttgcgagcat
     9721 atccaggcgg gtttttatcg gcataaccca ggcgttactc acaccgggga attgaatact
     9781 gctatttagc tcagcgatta aatccgccgt agttttaccg ctgcgccact ggctgcgggg
     9841 ttttaattga ataaaggttt ccagcatgct gatgggggct gggtcggtgg cggtttcggc
     9901 gcggcctgct ttgccgaata cgcgctccac ctccggcagt gcgctaatta ttttattggt
     9961 ttgttgtacc agctcgcggg ctttagcgat ggaaatgccc ggcgcgctag taggcatata
    10021 cattaaatcg ccttcatcca agggcggcat aaattcactg gccaaaaatt taagcggcat
    10081 taatgccgct acacctatga aacctacgca gagtaaaaat ggcgtgggtt ttactaaaca
    10141 gcgttttaat aggcgttggt aaagaataag caggtgtgtg tgcagccttg ccgcatgaac
    10201 atgcccagcg ggcagtttgc cgcttaggta atacaacacc accggcatca gggtaatggc
    10261 aataagggct gcgctggcca tggcgtaggt tttagtaagc gcaagggggc gaaataaacg
    10321 gccctcttca ccgtctaggg taaaaatcgg cataaaacta atggtgacga tcagtaaact
    10381 aaaaaataag gttgggccta cttccatagc gctgtcgtac accgattgca gatgatcggc
    10441 ggcggttttt tgtgtggcga ttctgcgctg gagattttcc accataacaa tggcgccatc
    10501 caccatggcg ccgagcgcaa tggcgatgcc gcctaaagac ataatattgg cgttaatgcc
    10561 ctgccagtgc atgagtaaaa aactcagcac aatacctatg ggcagggtaa tcagggcggc
    10621 aaaaactgac cgaacgctca ataaaaatac cagtaataaa atgcccagca agattaattc
    10681 ctgggccaat ttatgccaga gattattaac cgcacggcta atcaactggc tgcgatcata
    10741 aaccggtgta acctccacac ctgcaggtaa tgcaggagtt atttgggcga ttttttcttt
    10801 tactgcagca atagtttttt gcgcgttttc cccctggcgc atgacaataa tgccgccgac
    10861 aacttccccc cggccgttta aatccgcaag tcccctgcgc atggcggggg cttcaataat
    10921 ctcggccact tggtttaata aaagcggcgc gccgttttta tccataattc ccagcggcgt
    10981 ttggcctagg cttgctgcgc tggtgatagt gccggtggcc cgcagcatgg tttcagcttc
    11041 ggctaattca atggcggcaa ggcctgtctc gcggttgttg tgggtgacgg catttttaat
    11101 gtgcatgagg ggaatgccgt agagcattaa tttatttagg tccggctgaa tgtggtattc
    11161 ctttaccatg ccgcctatgg gtgccacttc agccaccccc ggcagggttt gtaattcgta
    11221 gcgcaaaaaa ttgtcctgca gggcgcgcaa ttgggctaaa tcctgttgat tatttttgct
    11281 gtgcagggcg tagatataaa cccaaccaac gccactggca tccgggccaa tttcggcgct
    11341 ggcgctgggc ggtaattttt tagccgcttg atttaaattg tccaacacgc ggctgcgggc
    11401 ccaataggta tccgtgccgt ctttgaaaat aacgtaaata aacgaatcgc caaaaaacga
    11461 aaacccccgc accgtgtggg cgccgggtac ggataacagc gcgctggtaa gcgggcgggt
    11521 gatttggctt tccactatat tgggcgcttg gcctgggtag ctggttttaa taatcacctg
    11581 cacatcggat aaatcgggga tggcatccac ggcggtggtg tgcagggcgc gcccgcccag
    11641 caaacacagc agcagcgtga tcaataaaac caacacccga ttgtgcaaac aggtgcgaat
    11701 aatccagccg atcatggcga ctcgtccgcc gcttttacat caattatttg aaatagcggt
    11761 gagccgtcgg tgatttttac gtttaattga tcgcccagtt taaaatgctc gatattaatt
    11821 gcgtctgtga gcgcaaaatt catggtcata ccgggcattt gccaggcggt aatagcttcg
    11881 tgttccagct gtattttacg ttcattggga aataattggc ttatgcgcgc cttcacccag
    11941 gtgggcggat aggcaggtgc cacttccggc gcctgcaggc gttttaaatc gctgctttta
    12001 ctggattcgg catcaattaa aaattgcgcc gaggtgacca ctatgtcccc ctcttcaacc
    12061 ccggccagca cctctactaa ttcttcattg cgcgcaccta aggtcacgcc gacgctttta
    12121 aatgtgccct cgggcgcttc cattaccagc cgcgcttggt tgtccagttc aatcacagcc
    12181 tggcgggaga ctaatagggc gggcgggcgc ggcggcaggc tgatcagccc ctgcatgagc
    12241 atattggggc gcagctgcat attggggttg gcaaggctca cccgcaggcg caggctgcgg
    12301 cttttggggt ctaagtccgg cgcaatggcg tccacctcgc cctcccacac cttgccgggc
    12361 aggctctggc tggtgaggct caccgcctgg cggggggcga ttaaggggcc ttggcttgcg
    12421 aacacctcca accacaccca cacccgctcc atggagccta tggccatgac catttggccg
    12481 gggctgaaat aggccttctc gcgcagtttg agcatgtcca ccacgccgtc tttgggggcg
    12541 tggaagatga cgctgcgctg ggccaggccc tgatctttaa ggcgtgccag gctgatgctg
    12601 tccatctgca ggtcttttaa atcctcctca gccttcatgg cttgggcggc cccttggcgg
    12661 cgggcagcca ggtagcgctc ctgggcctcg atgaggtctg gcgagtaaaa actaaacagc
    12721 ggctggcctt ttttgaccgc ttccccctcg ccgcttacaa acaacatatc cacccagcct
    12781 tggctgcggg ggctgatggg gctcaccagg ctcgggtccg ccaccacccg gccgaagctc
    12841 tgcagccgct ggttgagcac gccgcgggcc accttcacct tgcgcaccgc catctgctgc
    12901 tgcacggcgg ggttgatttt cactcgcccc gcctgctcag tcgctttggc gtcctcggca
    12961 tacaccggga ttaggtccat accatggggg catttgccgg gtttatcgct gcggtaattg
    13021 gggtccatgg aggaaaccca gtagaggggt ttgggggcgg tggcctgcca gggggcgaac
    13081 caggcgatgc tcgccaccag cagcgccata atccccagca gcgagaatct gtagcggcgc
    13141 agcggggcga taacggcagg cggggtttta gacatgacgt gttctcacgg gcaatgggca
    13201 gagctagggt ttggcactga tcgccaggcg aaagccgtag ctgctcttgc ggctggtgtg
    13261 gctgaagtag tagcggttgg ccgcgcgcat accgcttgcc gggtagtccc aggcgccgcc
    13321 tctgagcacg cgaatggcgc agttgtccgc aagccagggg ctgccatcgg taggcgcgcc
    13381 ctggtaattg gggtgccagc agtcctccac ccattcccac acattgccgt gtacatcaaa
    13441 caggccaaaa ccattggctt tgaaactttg caccggcagg ctgccgcggt ttttgccttc
    13501 ttttacccag caggggctgg cggcgccgcc gtcgaagcgg gcctgatcac agctggcgtt
    13561 ttgcccccac caatactcgc tgctggtgcc tgccctggcg gcgtattccc actcggcttc
    13621 gctgggcagt cggtagctgc ggccggtgag cttattcagc cagggcagaa attgctggtt
    13681 gatgtcctgg tagctgacat taatcaccgg ccgattgccc cgcccccagc cctcatccgg
    13741 cggcacgtaa taacagctgc tgctggccac acacaggtcg tacagggcaa agctgacttc
    13801 ggttttgctg aggctaaagg cgggcacctg cacctggtgt gcgggggttt catcactgct
    13861 ttccgggctg ccgcccatta aaaacccccc gccggggata gctaccatgg cggtttttag
    13921 ccaggcctcc acctgcaggc gggttttctc ctcttgaacg gccgcgtctt ttaaggcgtc
    13981 ctgccgccat ttggccatgg cggctatgtg gtcgttaaag gattcgcgca ccagggtttc
    14041 cgcctgctcc cagcgggcta gggcttcttt aaatttacct tgatcgcgct cctggcgggc
    14101 cagctccatc agcccatatg cctttaaggc cttggggggc agggcactgc gcacccgcat
    14161 ttggtaatac atcttggaat cggtgcgggt taattcggcg cgtttctccg ccgcaatgct
    14221 cggctcaatt aggttgtact gctcgattag cctgcggtat ccagctaaac gctgggctgt
    14281 ttgcgcaggc ttgccctggt ctaattcagc gcggctggag gtgcggaaca caaagcgctc
    14341 ggccagggct tggcgctgct taagctctgc aaggtctgcc acgttgctcc gttcggccgc
    14401 ttgcacggct tggttgaggg cggcttccag cttgattaac tcctggcggg cggtgtcaac
    14461 ttcctgctgt tgggcagatt ggggtgggat tgcggcgcct tgcgcggcgg caacttggtg
    14521 tgccgcccag aggttggcgg cggcgatgac cagcccgcca aggcgcttgc gcccgagggc
    14581 cgataagagc ggcggtagat aagccatgga gcccttctgc attatttttg tttaggtgga
    14641 attatcctac aaaaatcccg ggcgcctggc aggcatggct gggaggtttt ttaaagattg
    14701 tcgacccagt cctgatttat cgtccgatgg cgacaaatgc cgagttttgg cggccttttt
    14761 aagtcaaata gtcagatgaa atgcgccggt ataggggccg cttgagtagg tttttggcca
    14821 tttaagggtt tgccacggcc cttgaattcg gttcgcccgg tttcggtatt tttcagctaa
    14881 aaataactgc ccggagcgtg taaactctgc gccgcccgct ctacactcaa taaagccgct
    14941 ctaccttcgc tgtttgcaac gagatacccc atgtcagacc acacccggcc ccagcctttt
    15001 tgctggcgcg cgcattccat cagccacccc ggcagcgtgc gagcggccaa tgaggatgcg
    15061 gtgctgtgcc gggacgatgt gcagctgtgg gcggtggcgg acggcatggg gggccacaac
    15121 cggggggatc tggccagcaa taccctggtg cgccatttag agcgcaccga cgccagcccc
    15181 tccctggccg atgcggtaga cgccttagaa gacagcctgc taaacgccaa ccgggcggtg
    15241 ctcaactacg ctcaaaccca ttttcacggc gccatcatgg gcagcacctt tgtggcggtg
    15301 ctgattcgcg gcaatttagg ctttgtggc
//
LOCUS       DAOQGS010000018        15267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1787_length_15267_cov_5.861491, whole genome shotgun sequence.
ACCESSION   DAOQGS010000018 DAOQGS010000000
VERSION     DAOQGS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1787_length_15267_cov_5.861491"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..135
                     /locus_tag="PKZ52_01320"
     tRNA            52..135
                     /locus_tag="PKZ52_01320"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86..88,aa:Tyr,seq:gta)
     gene            240..313
                     /locus_tag="PKZ52_01325"
     tRNA            240..313
                     /locus_tag="PKZ52_01325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Gly,seq:tcc)
     gene            333..408
                     /locus_tag="PKZ52_01330"
     tRNA            333..408
                     /locus_tag="PKZ52_01330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:366..368,aa:Thr,seq:ggt)
     gene            399..1679
                     /gene="tuf"
                     /locus_tag="PKZ52_01335"
     CDS             399..1679
                     /gene="tuf"
                     /locus_tag="PKZ52_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNG58434.1"
                     /translation="MSSIIILKDPNPTFRGAQAMAKAKFERNKPHVNVGTIGHVDHGK
                     TTLTAALTRVCAETWGGQAVAFDGIDKAPEEKARGITISTSHVEYDSADRHYAHVDCP
                     GHADYVKNMITGAAQMDGAILVCGTTDGPMPQTREHILLSRQVGVPYIVVFLNKADLL
                     ADDCGGVGSDEYNEMLELVEMEIRDLLNTYEFPGDDTPIIAGSALMALEGKDDNELGV
                     SAVRKLVQALDSYIPQPERAIDKPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEVE
                     IVGIRATTKTTCTGVEMFRKLLDEGRAGENVGVLLRGTKRDEVERGQVLAKPGTIKPH
                     THFEAEVYVLSKDEGGRHTPFFKGYRPQFYFRTTDVTGACELPEGVEMVMPGDNIQMK
                     VTLIAPIAMEDGLRFAIREGGRTVGAGVVAKILA"
     gene            1989..2300
                     /gene="rpsJ"
                     /locus_tag="PKZ52_01340"
     CDS             1989..2300
                     /gene="rpsJ"
                     /locus_tag="PKZ52_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNG58435.1"
                     /translation="MTNQRIRIRLKAFDHKLIDASTQEIVETAKRTGAQVRGPIPLPT
                     RKERFTILISPHVNKDARDQYEIRTHKRLLDIVEPTEKTVDALMKLDLAAGVEVQISL
                     G"
     gene            2393..3037
                     /gene="rplC"
                     /locus_tag="PKZ52_01345"
     CDS             2393..3037
                     /gene="rplC"
                     /locus_tag="PKZ52_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNG58436.1"
                     /translation="MNIGIVGRKSGMTRLFTADGSSIPVTVIEVQPNRITQLKNLESD
                     GYTAVQVTVGSRKASRVVKAAKGHFEKAQVEAGASSWELRADSLEAFSVGGQITVEAF
                     SEGQIVDVTGQSKGKGFQGVIKRWNFSMQDATHGNSRSHRAPGSIGQNQTPGRVYKGK
                     KMAGHMGAERVTVQSLEIVKVDVERNLLLIKGAVPGAPGGDVIVRPAVKARKNG"
     gene            3056..3673
                     /gene="rplD"
                     /locus_tag="PKZ52_01350"
     CDS             3056..3673
                     /gene="rplD"
                     /locus_tag="PKZ52_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNG58437.1"
                     /translation="MEINIATANGAAAGTLSVSDVAFGREFNQDLVHQAVVAYMAGGR
                     QGSKAQKNRSDVSGGGRKPWKQKGSGRARAGSIRSPLWRGGGTTFAARPRNFDQKLNK
                     KMYRAAVQCILSELVRQDRLVLVEAFDVESPKTKSLLVKLNAMNLTNALIVGDAVSEN
                     LYLAARNLHNIDVRDAQAVDPVSLIRFDKVVVTVAALKKLEESLV"
     gene            3670..3966
                     /gene="rplW"
                     /locus_tag="PKZ52_01355"
     CDS             3670..3966
                     /gene="rplW"
                     /locus_tag="PKZ52_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNG58438.1"
                     /translation="MNQERIYKILLAPVVSEKTSVIAESSNQVVFKVIKNATKPEIKT
                     AIEKLFNVKVQNVQVLNVKGKTKRTRHGLGVKSDWKKAYVRLEAGQEFDLAVAE"
     gene            3980..4804
                     /gene="rplB"
                     /locus_tag="PKZ52_01360"
     CDS             3980..4804
                     /gene="rplB"
                     /locus_tag="PKZ52_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNG58439.1"
                     /translation="MTIVKRKPTSPGRRFVVSVVNADLHKGAPHAPLLEKKSKSGGRN
                     NAGRITTRHVGGGHKQHYRVIDFKRNKDGVPAVVERLEYDPNRTAHIALVCYADGERR
                     YIIAPKGLSAGDKIESGNAASIKVGNTLPLRNIPLGSNIHCVELKPGKGAQLARSAGA
                     SIQLVAKEGAYVTVRLRSGEMRKILSDCRATLGEVSNSEHNLRSLGKAGATRWRGVRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGVPTKGYKTRKNKRTTNMIVRRRTK"
     gene            4840..5115
                     /gene="rpsS"
                     /locus_tag="PKZ52_01365"
     CDS             4840..5115
                     /gene="rpsS"
                     /locus_tag="PKZ52_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNG58440.1"
                     /translation="MPRSLKKGPFIDHHLLKKVEVAVEKNDRRPIKTWSRRSMILPEM
                     VGLTIAVHNGKQHVPVSINEEMVGHKLGEFAATRTYRGHAADKKAKR"
     gene            5143..5475
                     /gene="rplV"
                     /locus_tag="PKZ52_01370"
     CDS             5143..5475
                     /gene="rplV"
                     /locus_tag="PKZ52_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNG58441.1"
                     /translation="MEVAAVLRGARLSAQKARLVADQVRGKGVEEALNVLTFSTKKGA
                     LLVKKVLESAIANAEHNEGADVDELKVSTIYVDEGLTMKRVKPRAKGRADRISKRTCH
                     ITVKVANK"
     gene            5489..6163
                     /gene="rpsC"
                     /locus_tag="PKZ52_01375"
     CDS             5489..6163
                     /gene="rpsC"
                     /locus_tag="PKZ52_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNG58442.1"
                     /translation="MGQKVHPTGIRLGIVKKHTSTWYAGNGQYADKLLIDLKVREYLQ
                     KSLAHASVSRIDIERPANTARVTIHTARPGIVIGKKGEDVEKLRAELSKRMGVPVHIN
                     IEEIRKPDLDAALTAQSIAQQLERRVMFRRAMKRAIQNAMRQGAEGVKIQVSGRLGGA
                     EIARTEWAREGRVPLHTLRADIDYSTAEALTTFGILGVKVWIFKGEIIGGIEEVEANQ
                     KKKGSK"
     gene            6176..6589
                     /gene="rplP"
                     /locus_tag="PKZ52_01380"
     CDS             6176..6589
                     /gene="rplP"
                     /locus_tag="PKZ52_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNG58443.1"
                     /translation="MMQPKRTKFRKQQKGRNRGLAQRGSKVSFGEFGLKATDRGRLTA
                     RQIEAARRAMTRHVKRGGKIWIRVFPDKPITQKPLEVRQGKGKGNVEYWVCQIQPGKV
                     LYEMEGVSEEMAREAFALAAAKLPITTTFVRRSVM"
     gene            6589..6780
                     /gene="rpmC"
                     /locus_tag="PKZ52_01385"
     CDS             6589..6780
                     /gene="rpmC"
                     /locus_tag="PKZ52_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNG58444.1"
                     /translation="MNAKDLSQKSVDELKALLLEQHQEQFKFRMQAVTGQLAQTHLLK
                     QNRRTIARIKTVLSQKAGK"
     gene            6783..7046
                     /gene="rpsQ"
                     /locus_tag="PKZ52_01390"
     CDS             6783..7046
                     /gene="rpsQ"
                     /locus_tag="PKZ52_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNG58445.1"
                     /translation="MSEVKTARTLTGRVVSDKMEKSVVVLIERRVKHPVYGKYISKSS
                     KLKAHDEHNECKVGDLVTIAESRPISKTKSWTLVKIDERAAAV"
     gene            7079..7447
                     /gene="rplN"
                     /locus_tag="PKZ52_01395"
     CDS             7079..7447
                     /gene="rplN"
                     /locus_tag="PKZ52_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNG58446.1"
                     /translation="MIQTQSYLEVADNSGARRVMCIKVLGGSHRRYANVGDIIKVSIK
                     EAIPRGKVKKGQVMKAVVVRTKSGVRRTDGSLIKFDDNAAVLLNNQDSPIGTRIFGPV
                     TRELRGEKFMKIISLAPEVL"
     gene            7474..7791
                     /gene="rplX"
                     /locus_tag="PKZ52_01400"
     CDS             7474..7791
                     /gene="rplX"
                     /locus_tag="PKZ52_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNG58447.1"
                     /translation="MRKIKRDDDVIVLTGRDKGKRGKVNKVLSDGKLIVSGIQIVKKH
                     QKPNPQAGVAGGIIEKEASIQASNVAIYNPSTKKADRVGFKILEDGKKIRIFKSTGAA
                     VDA"
     gene            7808..8347
                     /gene="rplE"
                     /locus_tag="PKZ52_01405"
     CDS             7808..8347
                     /gene="rplE"
                     /locus_tag="PKZ52_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNG58448.1"
                     /translation="MAKLKVQFKEEIAPRLMAELGLSNPMEIPKITKITVNMGVGEAV
                     GDKKVLEAAVGDLEKITGQKAVITKARKSIAGFKIREGWPIGCKVTLRSDKMYEFLER
                     LVSIAIPRIRDFRGISPKQFDGRGNFSMGVSEQIIFPEIDYDKIDRLRGLDICISTTA
                     RNDDEGRALLKAFNFPFKG"
     gene            8360..8665
                     /gene="rpsN"
                     /locus_tag="PKZ52_01410"
     CDS             8360..8665
                     /gene="rpsN"
                     /locus_tag="PKZ52_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNG58449.1"
                     /translation="MAKKSMIARETKRAKLVAKYAAKREALKTIVSSVTASEGEIWQA
                     ALQLQKLPRDASPSRIQRRCRVTGRPHAVYRKFGLCRHKLREAAMRGDVPGLVKASW"
     gene            8690..9082
                     /gene="rpsH"
                     /locus_tag="PKZ52_01415"
     CDS             8690..9082
                     /gene="rpsH"
                     /locus_tag="PKZ52_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNG58450.1"
                     /translation="MSMQDPVADMLTRIRNAQSVGKFSVTMPASKLKLSIAKVLQSEG
                     YIGSIEVSDDLKKVITLELKYFEGKPVISEVARVSRPGLRNYVAKTDLPTVRGGLGIA
                     IVSTSKGVMTDRAARAAGVGGEVICTVF"
     gene            9108..9641
                     /gene="rplF"
                     /locus_tag="PKZ52_01420"
     CDS             9108..9641
                     /gene="rplF"
                     /locus_tag="PKZ52_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNG58451.1"
                     /translation="MSRVAKNPVHLPSGVDVTLQGQSITVKGGKGTLNLNIHSSVEIV
                     REENVLTFVARNGDKDARAQAGCTRALVNNMVVGVSQGFEKKLQLVGVGYRAKATGSS
                     LSLTLGFSHPVEFEVPAGVSVETPTQTEVVLKSADKQLLGQVAAEIRSFRPPEPYKGK
                     GVRFADENVLRKEAKKK"
     gene            9653..10003
                     /gene="rplR"
                     /locus_tag="PKZ52_01425"
     CDS             9653..10003
                     /gene="rplR"
                     /locus_tag="PKZ52_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNG58452.1"
                     /translation="MNEKKESRLRRARSTRIRIRGLGVNRLSVHRTPRHIYAQIVSAT
                     GDVVLASASTLDKGISVEKTGNSSAAQSVGKLIAERAKAAGITSVAFDRSGFRYHGRI
                     KALADAAREAGLEF"
     gene            10014..10523
                     /gene="rpsE"
                     /locus_tag="PKZ52_01430"
     CDS             10014..10523
                     /gene="rpsE"
                     /locus_tag="PKZ52_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNG58453.1"
                     /translation="MSTNEKEKSVNDGLQEKLVQVNRVSKTVKGGRIFAFTALTVVGD
                     GNGRVGFGRGKAREVPIAIQKAMEAARRNMITVELSGDTLHYATKGRHGASKVFMQPA
                     SQGTGIIAGGAMRSVLEIAGVQNVLAKCYGSTNPVNVVRATFQALADMSSPEYVAAKR
                     GKSVDEILN"
     gene            10561..10743
                     /gene="rpmD"
                     /locus_tag="PKZ52_01435"
     CDS             10561..10743
                     /gene="rpmD"
                     /locus_tag="PKZ52_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNG58454.1"
                     /translation="MSKKLVKVTQTKSAAGRLAAHKACLIGLGLRRIGHTVEVEDTPS
                     VRGMINKVSYMLKIEG"
     gene            10747..11187
                     /gene="rplO"
                     /locus_tag="PKZ52_01440"
     CDS             10747..11187
                     /gene="rplO"
                     /locus_tag="PKZ52_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNG58455.1"
                     /translation="MRLNSLSPAPGRIQAPKRVGRGIGSGLGKTSGRGHKGQKARKSG
                     NPRPGFEGGQMPLQKRLPKYGFTSRVGRVSAEVRLDELNKVAGNEITLKTLLEADVIQ
                     AGIKRAKIFLSGEITKAVTVSGVAVTKGAKAAIEAAGGKVVVEE"
     gene            11196..12539
                     /gene="secY"
                     /locus_tag="PKZ52_01445"
     CDS             11196..12539
                     /gene="secY"
                     /locus_tag="PKZ52_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNG58456.1"
                     /translation="MAGPNALSMTNQKGLGELWARLRFLLLAILVYRIGTHIPVPGID
                     PEQVARLFNQNKNTILAMFNMFSGGALERMSILALGITPYISASIIVQMLTSVVPALE
                     QLKKEGDSGRRMISQYTRWLTVGLALVQSFIMVAGMHSQGMAYADLPMMSFYLVATTS
                     IVTGAVFLMWLGEQVTERGIGNGISMLIFAGIVAGLPRAAGQAIESARQGDLNWLLLL
                     VIAILAVAIVWFVVFIERGQRRITIHYAKKQQGRFAHTQQTSHLPLKINMAGVIPVIF
                     ASSLLLFPASIAQWFGQSSTGKISELFQEFALLIGPGQPLNFILFAGLIILFCFVYTA
                     MMYNPKEVAENLKRGGAYIPGIRPGEHSAKYIDTVLTRLTLVGAVYMAGVALLPQFLV
                     VATKVPFYLGGTSLLIVVVVVMDFMSQVQSHLMSHQYESVMKKANLKNYGSSSAR"
     gene            12552..12668
                     /gene="rpmJ"
                     /locus_tag="PKZ52_01450"
     CDS             12552..12668
                     /gene="rpmJ"
                     /locus_tag="PKZ52_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNG58457.1"
                     /translation="MKVRTSVKAVCRNCKIVRRKGVLRVICSAEPKHKQRQG"
     gene            12872..13228
                     /gene="rpsM"
                     /locus_tag="PKZ52_01455"
     CDS             12872..13228
                     /gene="rpsM"
                     /locus_tag="PKZ52_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNG58458.1"
                     /translation="MARIAGVNIPDNKHAVISLTYVYGIGRTTAKRICASTGIAEDAK
                     ISSLTEDQIDLIRNEVTKITVEGDLRREVSMSIKRLMDLGCYRGLRHRKGLPVRGQRS
                     KTNARTRKGPRKAIKK"
     gene            13260..13655
                     /gene="rpsK"
                     /locus_tag="PKZ52_01460"
     CDS             13260..13655
                     /gene="rpsK"
                     /locus_tag="PKZ52_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNG58459.1"
                     /translation="MAKPSAKSAPRKKVKKTVVDGVAHIHASFNNTIVTITDRQGNTL
                     SWATSGGSGFRGSRKSTPFAAQVAAERAGQAALEYGLKNLDVEVKGPGPGRESAVRAL
                     NNVGYKITNIVDVTPIPHNGCRPPKKRRV"
     gene            13667..14287
                     /gene="rpsD"
                     /locus_tag="PKZ52_01465"
     CDS             13667..14287
                     /gene="rpsD"
                     /locus_tag="PKZ52_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNG58460.1"
                     /translation="MARYIGPTCKLSRREGTDLFLKSGARALDSKCKLETAPGQHGQR
                     RGRLSDYGVQLREKQKVRRIYGILEKQFRGYYKEAARRKGATGENLLQLLECRLDNVV
                     YRMGFGATRAESRQLVSHKSLTVNGKVVNIPSYQVQVGDVVAVREKAKNQLRIQSALA
                     LSAQRADIDWIDVNTEKKEGVFKRVPDRGDLSADINENLIVELYSK"
     gene            14353..>15267
                     /gene="rpoA"
                     /locus_tag="PKZ52_01470"
     CDS             14353..>15267
                     /gene="rpoA"
                     /locus_tag="PKZ52_01470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNG58461.1"
                     /translation="MLLAVNEFLTPRHIDVTELAPTRARVVLEPLERGFGHTLGNALR
                     RILLSSMPGCAITEVEIDGVQHEYSAIEGIQEDVIEILLNLKNVAIVMHGKDHAVVTL
                     SKKGPGIVTAGDITVDQSVEIKNPDLLIAHITGDVALNMRLTITRGRGYQPADARESS
                     DDETRTIGRLHLDASYSPVRRLAYSVESARVEQRTDLDKLVIDLETNGTIDPEEAIRR
                     AATILQQQLAVFVDLEGEKESEPEKKEDLIDPILLRPVDDLELTVRSANCLKAENIYY
                     IGDLIQRTEVELLKTPNLGKKSLTEIKDI"
BASE COUNT         3980 a         3599 c         3929 g         3759 t
ORIGIN      
        1 gtcgggggtt cgattcctct cgccagctcc ataaaaaatc gaaaatattc cgcaggggtt
       61 cccgagtggc caaagggatc agactgtaaa tctgacacga aagtttcggt ggttcgaatc
      121 cacccccctg caccatatcc atgtcgagat gctcggcata acccgctgat ttaacgcttt
      181 ggttcaaacg ccctggcttg agttggcggt cacccggtta aatgggtggg ctaataaaag
      241 cgggcatagt ttagtggtaa aaccctagcc ttccaagcta gctatgcggg ttcgattccc
      301 gctgcccgct ccaaatgtgt gttgtgaatt atgctcatgt agctcagttg gtagagcaca
      361 cccttggtaa gggtgaggtc accggttcaa ttccggtcat gagctccatt attatcctta
      421 aagatccaaa tcccaccttc agaggagcgc aggcgatggc aaaggctaaa tttgaacgta
      481 acaaacccca cgtaaacgtc ggcaccattg gccacgtaga ccacggtaaa acgacgctga
      541 cagcagctct tactcgcgtc tgcgcagaaa cctggggtgg ccaagcggtt gcttttgacg
      601 gcatcgacaa agcgcctgaa gaaaaagccc gtggtatcac catctcgact tcacacgtgg
      661 aatacgactc agctgatcgt cactacgccc acgttgactg cccaggccac gccgactacg
      721 tgaaaaacat gatcaccggt gctgcccaga tggacggcgc gatccttgtg tgcggcacta
      781 ctgacggccc tatgccacaa acccgcgaac acatcctgct gtctcgccaa gtgggcgtac
      841 cctacatcgt ggtattcctg aataaagctg acctgttggc tgacgactgc ggcggcgtag
      901 gctctgacga atacaacgaa atgttagaac tggttgagat ggaaattcgc gacttgttga
      961 acacctacga attcccaggc gacgacactc ccatcatcgc aggctctgcc ctgatggctc
     1021 tggaaggcaa agacgacaac gaactgggcg tatccgctgt tcgcaaactg gtacaggcgc
     1081 tggattccta catcccacag ccagagcgcg ccatcgacaa gccattctta atgccaatcg
     1141 aagacgtatt ctccatctct ggtcgcggta ctgtagtcac tggccgtgta gagcgtggca
     1201 ttgtaaaagt gggcgaagaa gttgaaatcg tcggcattcg cgccaccacc aaaaccacct
     1261 gtaccggtgt tgaaatgttc cgcaaactgc tcgacgaagg tcgcgctggt gagaacgtcg
     1321 gtgttttgtt gcgcggcacc aagcgtgacg aagttgagcg cggtcaagta ttggccaagc
     1381 ccggcaccat caagccgcac acccatttcg aagcagaagt gtacgtattg tccaaagacg
     1441 aaggtggccg tcacacgccc ttcttcaaag gctaccgtcc acagttctac ttccgtacta
     1501 ccgacgttac tggcgcctgt gagctgccag aaggcgttga aatggttatg cctggcgaca
     1561 acatccaaat gaaagtgacc ctgatcgcac cgattgcgat ggaagacggt ctgcgctttg
     1621 cgatccgcga aggtggccgt actgtcggcg cgggcgttgt agctaaaatc ctcgcttaat
     1681 taatattctg cgttgaataa taaaaggggg ccgcaggccc cttttttatg tctgatagct
     1741 tttccgatag ccgtttttgt gcagatcgtg ttggcgcctc tggccgttga ttttctttat
     1801 ctttgaatag gctgcaggga tcggttagtg aaaaaagcat cccctgcaca gctcggcaaa
     1861 ataattgcaa ataaggcttg acggggtggg ggggcataca tagaattgcg cctccatttt
     1921 gtgggtgccc agttagatgt tttggcacag catgaaagtt ctttaaaaat tctggagttt
     1981 tggttcacat gacgaatcag cgtattcgaa ttcgcttgaa ggccttcgat cataaattga
     2041 ttgatgcctc cacacaagaa atcgtggaaa ctgcaaagcg taccggtgcc caagtgcgcg
     2101 gacctattcc gctgcctacc cgtaaagagc gctttaccat tttgatctcc ccgcatgtta
     2161 ataaagatgc gcgtgatcaa tacgaaatcc gcactcacaa gcgtctgtta gacattgttg
     2221 agccaactga aaagactgtt gacgcactta tgaagctgga tcttgcagct ggtgtggaag
     2281 ttcaaattag tcttggttaa aactccaagc cagccctgta aacagggtgt gtaacgctct
     2341 gaaatgggcg gccatagcgg gtaagagccc cgtacaccaa gaggtcataa aaatgaatat
     2401 cggtattgtc ggccgcaaaa gcggtatgac acgtcttttt acagcagatg gttcgtccat
     2461 ccctgttact gtgattgagg ttcagcccaa tcgcatcact cagcttaaaa acctagagtc
     2521 cgatggctat acagctgtgc aagtgactgt gggttctcgc aaagcgtctc gcgtcgttaa
     2581 agccgctaaa ggtcacttcg aaaaagccca ggttgaagcc ggtgcttcca gttgggaact
     2641 gcgtgctgat tccctagagg ctttctctgt tggcggtcaa attacggttg aagccttctc
     2701 agaaggtcaa atcgttgatg ttacaggtca gtccaaaggt aagggtttcc agggcgtaat
     2761 caagcgctgg aatttctcca tgcaggatgc gactcacggt aactctcgtt ctcaccgtgc
     2821 gccaggctcc attggtcaaa accaaacccc aggtcgcgtt tacaaaggca aaaaaatggc
     2881 cggtcacatg ggcgctgagc gcgtgactgt tcagagcctt gaaattgtta aagtcgacgt
     2941 agagcgcaac cttcttttaa taaaaggtgc ggttccaggt gctcccggtg gtgacgtgat
     3001 tgtacgtcct gcggttaaag cgcgtaaaaa cggttaattt ccgagggggt cgtccgtgga
     3061 aatcaatatt gcaacagcta acggtgctgc tgctgggacc ctgagtgttt cagacgttgc
     3121 atttggtaga gagtttaatc aagacctagt gcatcaagcc gtcgtcgcct atatggctgg
     3181 cgggcgtcag ggtagcaaag cacagaaaaa ccgctccgat gtttccggtg gcggcagaaa
     3241 accctggaaa caaaagggtt ccggtcgggc ccgtgcgggt agtatccgca gcccgttgtg
     3301 gagagggggc ggcaccacgt tcgcagctcg tccacgcaat ttcgatcaga aactaaataa
     3361 gaaaatgtat cgcgccgctg tgcagtgcat tttgtctgag ttggttcgcc aagatcgctt
     3421 agttctggtt gaggcatttg acgttgagtc gcccaaaacc aaatctttgt tggttaaatt
     3481 aaacgcaatg aatctaacta atgcgttaat tgttggcgac gctgtgagcg aaaatttgta
     3541 tttggctgct cgtaacctac acaacattga cgtgcgagac gctcaggcag ttgatcctgt
     3601 cagcttgatt cgtttcgaca aagtagtcgt gaccgtggca gccctcaaaa aacttgagga
     3661 gtctctggta tgaatcaaga gcgcatctat aagatcttgt tagcgcccgt ggtgtccgag
     3721 aaaacctcgg ttatcgcaga gtcgtctaac caagtcgtgt tcaaagtcat taagaatgcc
     3781 actaagcctg aaatcaaaac cgccattgaa aaattgttca atgtgaaggt gcaaaatgtt
     3841 caggtattaa acgtcaaagg taagactaag cgtactcgcc atggtttagg cgtgaaaagc
     3901 gattggaaaa aagcctacgt ccgtcttgaa gcaggtcaag aatttgacct agctgttgct
     3961 gaataaggag tgcgtagaca tgactatcgt taagcgtaaa cccacatctc ctggtcgtcg
     4021 ttttgtcgta agcgtcgtca atgccgatct tcacaagggt gctcctcatg cccctttgct
     4081 tgagaagaag agcaagtctg gcggtcgtaa caacgccggc cgtatcacca cccgccatgt
     4141 tggcggtggt cataagcaac actatcgagt gatcgatttt aaacgcaata aagatggcgt
     4201 tcccgctgtt gttgagcgct tggaatacga tccaaaccgt accgctcaca ttgcattggt
     4261 atgttatgcc gatggtgagc gccgctatat catcgcgcca aaaggtttga gcgcgggcga
     4321 taaaattgaa tccggcaatg ctgcatctat taaggtcggc aacactctgc cactgcgcaa
     4381 tatccccctg ggaagcaata tccactgcgt ggaattgaag cctggcaaag gtgctcagtt
     4441 ggcccgatcc gctggagcca gcatccagtt agtagccaaa gaaggtgctt acgttactgt
     4501 gcgtcttcgc agcggtgaaa tgcgcaaaat tctgtctgac tgccgcgcca ctttaggcga
     4561 agtgtcaaac agtgagcaca acctgcgttc cctaggtaaa gctggtgcaa cacgttggag
     4621 aggtgtacgc ccaaccgttc gtggtgtggc catgaaccca gttgatcacc cgcacggtgg
     4681 tggtgaaggt cgtacatcgg gtggccgtca cccagtgtct ccatggggcg ttcctacgaa
     4741 gggttataaa actcgtaaga acaagcgtac cactaacatg attgtgcgtc gtcgcaccaa
     4801 gtaattggcg acgattaact agctagcgag gaatttgcag tgccacgttc actgaagaaa
     4861 ggccctttca tcgatcatca cttgttgaaa aaagtggaag tcgccgtaga gaaaaacgat
     4921 cgtcgcccca tcaaaacctg gtcgcgccgc tcaatgatcc tgcctgaaat ggtaggtcta
     4981 acaatcgccg tccataacgg caaacagcat gtgcccgtgt ccattaacga agaaatggtc
     5041 ggtcacaagc tgggtgagtt tgcagcaaca cgtacctatc gtggtcacgc tgctgataaa
     5101 aaagctaagc gttaatcaga ccggcatacg agggtaatcg agatggaagt agcagcagta
     5161 ttacgcggtg cacgtctatc agcgcagaaa gctcgcctgg ttgcggatca agttcgcggc
     5221 aaaggtgttg aagaagcact gaatgtattg acgtttagca ctaaaaaggg cgctttgctg
     5281 gttaagaaag tgcttgagtc cgccatcgcc aacgccgagc acaacgaagg cgccgatgta
     5341 gacgaattaa aagtatccac catttatgtg gatgaaggtt taaccatgaa gcgggtcaag
     5401 ccgcgcgcga agggtcgagc tgaccgaatt tctaagcgca cgtgccatat caccgtcaaa
     5461 gtagccaaca aataagagag cggacgttat gggtcagaaa gtacatccga cgggtattcg
     5521 tctcggaatt gttaaaaagc acacttcgac ttggtatgca ggcaacggcc agtacgctga
     5581 taagttattg atcgatctta aagtgcgtga gtatttgcaa aagtcactcg cccacgcttc
     5641 ggttagccgc atcgatatcg agcgccctgc caacaccgct cgtgtgacta ttcacactgc
     5701 gcgcccaggt attgtgatcg gtaaaaaagg cgaagacgtt gaaaaattgc gcgctgaatt
     5761 atctaagcgt atgggtgtgc ccgtgcacat caatatcgaa gaaattcgca agccagattt
     5821 agatgcagcc ttaactgctc agagcattgc tcaacagtta gagcgccgcg ttatgttccg
     5881 tcgcgctatg aagcgtgcga ttcagaacgc catgcgccaa ggtgctgaag gcgtaaaaat
     5941 tcaagtgagc ggacgtttgg gcggggctga gattgctcgt accgaatggg ctcgagaagg
     6001 tcgtgtgccc ttacacacgc tgcgtgcgga tattgactat tccaccgctg aagcactaac
     6061 cacgttcggt atcctaggtg tgaaagtttg gatctttaaa ggcgagatca taggcggcat
     6121 cgaagaagtc gaagctaatc aaaagaaaaa aggttctaag taaggggtac gccatatgat
     6181 gcaacctaag cgtacgaagt ttcgtaagca acagaagggc cgcaaccgtg gccttgctca
     6241 acgcggctcg aaagttagct tcggcgaatt tggattaaaa gcgactgatc gcggccgttt
     6301 gacagctcgt caaattgaag cagcccgtcg cgcaatgact cgtcacgtta agcgtggcgg
     6361 taaaatttgg attcgtgtgt tcccggacaa gccaattacc cagaagcctt tagaagttcg
     6421 tcaaggtaag ggtaagggta acgttgaata ttgggtatgc caaattcagc cgggtaaagt
     6481 cctgtatgaa atggaaggtg tttcagagga aatggcgcgc gaagcgtttg cccttgccgc
     6541 tgcaaaactt ccaattacaa ccacatttgt tagacggtcg gtgatgtaat gaacgccaaa
     6601 gatctcagcc aaaaatccgt tgacgagttg aaagcgcttt tgttggagca gcaccaggag
     6661 caatttaagt tccgtatgca agctgtcacc ggacaactgg ctcaaaccca cttgttaaag
     6721 caaaaccgcc gcaccattgc gcgcattaaa actgttctgt cacaaaaggc aggtaaataa
     6781 ccatgagtga agttaaaacc gcacgaacac tcaccggccg agtagtgagt gacaaaatgg
     6841 agaaatccgt tgttgtctta attgagcgtc gcgtaaagca cccggtttac ggcaaataca
     6901 tcagcaaatc gtccaagtta aaagcacacg atgaacacaa cgagtgcaaa gtaggcgatt
     6961 tagttaccat cgcagagtct cgcccgattt caaaaactaa gtcttggact ttagtgaaaa
     7021 tcgatgagcg cgctgccgcg gtttaatcag cgctcaaatt aggtattgga gataaacaat
     7081 gattcagaca caatcctatt tggaagttgc tgacaatagc ggtgcacgtc gcgtgatgtg
     7141 tatcaaggtg ttgggcggtt cacaccgtcg ttatgccaat gtcggcgaca tcatcaaagt
     7201 gagcattaaa gaagcgattc cacgcggcaa agttaaaaaa ggccaagtga tgaaagcagt
     7261 tgtggtgcgc accaaatccg gtgtgcgccg tactgatggc tctttaatta agttcgacga
     7321 caacgctgca gtgttgctca acaaccaaga ctcgcccata ggtactcgta tctttggacc
     7381 agttacccgc gagctgcgcg gcgaaaaatt catgaaaata atttctttgg cgccagaagt
     7441 tctgtaagtg gcctgaggtt ttgaggatcg gttatgcgta aaattaagcg tgatgacgac
     7501 gttatcgttc tcactggccg cgacaaaggc aagcgcggca aagtaaacaa agtattgtct
     7561 gatggcaaat tgattgtatc tggtatccag atcgtcaaaa aacatcaaaa gcctaatcct
     7621 caagcgggcg tcgctggcgg aattatcgaa aaagaagcat cgatccaggc ttctaatgtg
     7681 gctatctata atccatcaac caaaaaagct gatcgcgttg gttttaagat tctggaagac
     7741 ggcaaaaaaa tccgtatttt caaatctacc ggcgctgcgg tagacgcata agcgcattga
     7801 gagagttatg gctaagttaa aagtccagtt taaagaagaa atcgccccgc gcctgatggc
     7861 cgaattgggt ttatccaatc cgatggagat accaaaaatc accaaaatca ctgttaatat
     7921 gggtgtgggt gaagcggtag gcgacaagaa agtgttggaa gctgcggttg gtgatttgga
     7981 gaaaattact ggtcaaaagg ctgttatcac taaggcgcgt aaatccatcg ccggtttcaa
     8041 aatccgcgaa ggttggccca ttggttgtaa agtaactctg cgcagcgaca aaatgtacga
     8101 attcttggag cgtttggtat ccatcgctat cccacgtatt cgcgacttcc gcggcattag
     8161 ccccaagcag ttcgacggtc gcggcaactt ttctatgggt gtcagcgagc aaattatttt
     8221 ccctgagatc gattacgata aaattgatcg cctgcgcggc ttggatattt gtatatccac
     8281 caccgcgcgc aatgatgatg aaggccgagc tctgctgaaa gcctttaatt ttccattcaa
     8341 aggttaatcg gggaatttta tggccaagaa atccatgatt gcgcgcgaaa ccaaacgcgc
     8401 aaaactagtt gcaaaatatg ctgcaaaacg tgaagccctg aaaactatag tcagcagcgt
     8461 aaccgcttct gaaggtgaaa tttggcaggc cgctctgcag ctgcaaaaac ttccacgaga
     8521 tgctagcccc tcgcgcattc aacgtcgatg ccgagtgact ggccgtccac atgctgtgta
     8581 tcgcaaattt ggcctttgcc gccacaaatt gcgtgaagct gccatgcgtg gtgacgtacc
     8641 agggcttgtg aaagctagct ggtaaactac cattccggag atttaattaa tgagtatgca
     8701 agacccagtt gccgacatgc ttacgagaat tcgtaacgct cagtcggtag gaaaattttc
     8761 tgtcactatg cctgcttcta agctgaagtt gagcatagct aaagttctgc aatctgaagg
     8821 ctacatcggt tccatcgaag tctcagacga cctgaaaaag gttattacct tggaacttaa
     8881 atatttcgaa ggcaagcctg ttatttctga ggttgcccgt gtaagtcgtc ctggtcttcg
     8941 caattatgtt gccaaaactg acctgccaac ggtgcgcggc ggtttaggta ttgccatagt
     9001 ttctacgagc aagggcgtaa tgaccgaccg tgctgcacgc gcagcgggtg ttggtggtga
     9061 agtgatttgc actgtctttt gatgaataga tttaacaggt aattgttatg tcacgtgttg
     9121 caaaaaatcc tgttcacttg ccaagcggtg ttgacgttac gctccaaggt cagtcgatca
     9181 ctgtaaaagg tggtaaggga acattaaatt taaatataca ttccagcgtg gaaattgtgc
     9241 gtgaagaaaa cgtactgact tttgtggccc gcaatggcga caaagatgcc cgcgctcaag
     9301 ccggttgcac acgcgcactg gtgaacaaca tggttgttgg cgtaagccaa ggttttgaga
     9361 aaaagttgca gttggtgggt gtaggttatc gtgctaaagc cactggttct tctcttagct
     9421 tgacgcttgg tttttcacac ccagttgaat ttgaagttcc agccggcgtt tcggtggaaa
     9481 ctccaactca aactgaagtg gtgttgaaaa gtgcagacaa gcaattgttg gggcaggtag
     9541 ccgctgaaat tcgcagcttc cgtccaccag agccttacaa gggcaagggt gtgcgttttg
     9601 cggatgaaaa cgtactgcgt aaagaagcta agaagaagta aggcttaaga ctatgaatga
     9661 gaaaaaagaa tcgcgtttgc gccgtgctcg cagcactcgc atcagaattc gtggcttagg
     9721 cgttaatcgt ctgagtgtgc accgcacacc tcgccacatc tacgcacaaa ttgtttcggc
     9781 aactggcgat gtggtattgg cttctgcctc aactctggac aaaggtatat ctgtcgagaa
     9841 aacaggcaac agctccgctg cccaaagcgt gggtaagttg atcgctgaac gtgcgaaagc
     9901 cgctggtatt accagcgttg cattcgaccg cagcggattt cgctatcacg gtcgcatcaa
     9961 ggcgcttgcc gatgccgcgc gtgaagctgg gttagagttc taagaggccg tctatgtcta
    10021 ctaatgaaaa agaaaaaagc gttaacgacg gccttcaaga gaagttggtg caagttaatc
    10081 gtgtatctaa aaccgtaaaa ggtggccgta tcttcgcctt taccgcgctg acagtagtcg
    10141 gtgatggcaa tggccgcgtc ggttttggcc gaggtaaagc tcgtgaagtg cctattgcca
    10201 ttcaaaaggc aatggaagcg gcgcgccgca atatgattac cgttgagttg agcggcgata
    10261 ctttgcatta cgctaccaaa ggccgccatg gtgcttccaa agtgtttatg cagccagcct
    10321 cgcaaggtac cggaatcatc gccggtggtg ctatgcgctc tgttttagag atcgcgggtg
    10381 tacaaaacgt actggctaag tgctacggct caactaatcc tgtaaacgtg gtgcgcgcta
    10441 cctttcaagc cctggccgat atgtcctcgc ctgaatacgt agctgctaag cgtggcaaat
    10501 cagttgacga gattctgaat taatctctcc acctgacgtg tcttaagcgg aatacttaat
    10561 atgtctaaga aattagtaaa agtaactcaa acgaaaagtg ccgccggtcg cctggctgca
    10621 cacaaagctt gtctgatagg cttgggcttg cgtcgcatcg gccacactgt ggaagttgaa
    10681 gacaccccat ccgttcgcgg catgatcaat aaagtcagct atatgctgaa aatcgagggt
    10741 taatccatgc gtctaaatag cttaagccca gctcctggcc gcattcaagc gcccaagcgt
    10801 gtaggtcgtg gcattggctc tggccttggt aaaacctcag gccgcggtca caaaggtcaa
    10861 aaggctcgca agagcggcaa cccgcgtcca ggttttgaag gcggtcagat gcctttgcaa
    10921 aaacgcttac ctaaatacgg ctttaccagc cgtgtaggcc gcgttagcgc agaagtgcgt
    10981 ttggatgaac tcaataaagt agccggtaac gaaattaccc tgaaaactct gttagaggct
    11041 gatgttatcc aagccggtat caaacgcgcc aagattttct tgtccggcga gatcaccaag
    11101 gctgtgactg tatcaggtgt tgctgttact aaaggcgcca aagcggccat cgaagctgcg
    11161 ggcggcaaag tagtggttga ggaataatcg tacacatggc tggccctaac gcattatcta
    11221 tgacaaatca gaaagggttg ggcgagctat gggctcggct ccgcttcttg ttgctggcca
    11281 tattggtgta ccgcattggt acccatatac cggtgccagg catagacccg gagcaggtag
    11341 cgcggttatt taatcaaaac aaaaacacca tccttgccat gttcaacatg ttctcgggtg
    11401 gtgcgcttga gcgtatgagt attttggcgc tcggtattac gccctatatc tctgcctcga
    11461 ttatcgtgca gatgctgaca tccgttgttc ccgcgctgga gcagttaaag aaagaaggtg
    11521 actccggccg ccgcatgatc agtcagtaca ctcgttggtt gacggttggc ctggcactcg
    11581 tacaaagctt tatcatggtc gccggcatgc attcacaggg catggcctac gccgatttgc
    11641 cgatgatgtc gttctattta gttgctacta cctctattgt gactggagcc gtgttcttga
    11701 tgtggctcgg cgagcaggtg accgagcgcg gtattggcaa cggtatctcc atgctcatct
    11761 tcgcaggtat tgtggcaggc ttgccacggg cagccggtca agcgattgag agcgcccgcc
    11821 aaggtgatct caattggctg ttgcttctgg taattgccat tctggctgtt gcaattgtgt
    11881 ggtttgtggt ctttatcgag cgcggccaac gccgaatcac cattcattat gcaaaaaaac
    11941 aacagggtcg ctttgcccat actcagcaga cgagccatct gcctttgaag atcaatatgg
    12001 cgggtgtgat cccggtgatt ttcgcctcaa gcctacttct atttcctgcc tccatcgcac
    12061 aatggttcgg tcagagctct acaggtaaaa taagtgagct atttcaggaa tttgctcttc
    12121 tcataggccc aggacaaccg ttaaacttca tcctctttgc cgggctgatt atcctcttct
    12181 gtttcgttta tacagccatg atgtataacc ccaaagaagt ggctgaaaac cttaaacgtg
    12241 gcggtgctta tatcccgggt attcgtccgg gtgaacattc cgccaaatac atagatactg
    12301 tgcttactcg tttgacgctg gtgggcgctg tatacatggc tggggttgcg ttattacctc
    12361 agttcctggt tgttgctacc aaggtgccgt tttatttagg cggcacctcg ttgctgatcg
    12421 tagttgttgt ggtgatggac tttatgtccc aagtgcaatc gcatttgatg tcccaccaat
    12481 acgagtcagt gatgaaaaaa gcaaatttga aaaactatgg cagtagctct gccagataat
    12541 tgaggttaac aatgaaagtt cgtacttctg ttaaagccgt ttgccgcaat tgtaaaattg
    12601 tgcgtcgcaa aggtgttctg cgtgttatct gcagcgccga acctaagcac aagcaacgtc
    12661 aaggctaatt tggccgcaag gccagcccga ttgagttgtt caatcggttt aggggtgtta
    12721 actgtcgcaa ggtaaattca actatttact ctgctaaaaa aattagtgat ttctataagt
    12781 taattcgaga aaccccaagc tttcttgggg tttcttttta cttgttaggg ttgctgttaa
    12841 cacacaaatt tttaccatct ggagttaaaa aatggctcgt atcgctggtg tcaacatacc
    12901 agacaacaaa cacgccgtca tttccctgac ctacgtatac gggattggcc gcaccaccgc
    12961 taagagaatt tgcgcctcta ctggcattgc cgaagacgct aaaatctctt ccttaactga
    13021 agaccaaatc gacttgattc gtaacgaagt tactaaaatc acagtagaag gtgatttgcg
    13081 tcgtgaagtg tctatgagta ttaagcgcct gatggacctc ggttgttacc gcggacttcg
    13141 tcaccgtaag gggcttcctg tgcgtggtca gcgttcaaag acaaacgctc gaactcgtaa
    13201 aggccctcgc aaggcaatta agaaataatt aaccctcgct actcatagga atatttccaa
    13261 tggcaaagcc aagtgctaaa tcagcaccgc gcaagaaggt caaaaagacc gtagttgacg
    13321 gtgttgccca tattcatgct tcatttaaca ataccattgt gaccatcact gatcgtcaag
    13381 gtaatacctt gagctgggct acttccggcg gatcaggctt ccgtggttca cgtaaaagta
    13441 ctccattcgc cgctcaggtt gctgccgagc gcgcaggtca agctgcactg gaatacggtt
    13501 tgaaaaacct ggacgtagaa gttaaaggtc ctggccccgg ccgtgaatcc gctgttcgtg
    13561 cgttgaataa tgttggttac aaaatcacta acatagtgga tgtaaccccc ataccgcaca
    13621 acggttgccg cccacccaaa aagcgtcgtg tataggagac tgattcatgg ctcgttatat
    13681 tggacctact tgtaaattat cccgccgcga aggcactgat ctctttttaa agagcggtgc
    13741 gcgtgcgtta gattcaaaat gtaaattaga aaccgctccc ggccaacacg gccagcgccg
    13801 cggccgtctg tctgactacg gtgtacagtt gcgtgaaaag caaaaagtac gtcgtatcta
    13861 cggaatcttg gaaaaacaat tccgcggcta ctataaagaa gctgcccgcc gtaagggcgc
    13921 aaccggtgaa aacctgttgc aactactgga gtgccgttta gacaacgtgg tgtatcgtat
    13981 gggttttggt gctactcgcg ctgagtctcg tcaattggta tcccataaat cgctgacagt
    14041 aaatggcaag gttgtgaata ttccctccta ccaagttcaa gttggcgatg tggttgcagt
    14101 ccgtgaaaag gcaaagaatc agttgcgtat tcaaagcgca ttggctctgt ctgctcagcg
    14161 cgctgacatt gattggattg acgttaatac cgagaaaaaa gaaggcgtgt ttaaacgcgt
    14221 gcccgatcga ggtgatttgt ccgccgacat caatgaaaac ctgattgtgg aactttactc
    14281 taagtaacgc ttgaatatct ctggcattta ttaacctggt tgcaaagcca ggttctaaca
    14341 taggtattaa ctatgctgtt agctgtaaat gaatttttga ctccgcgtca tattgatgtc
    14401 actgagcttg cgcctactcg cgcccgtgtg gttttagagc cactggagcg cggttttggt
    14461 cacacgttgg gcaatgccct gcgccgcatt ctattgtcat caatgcccgg ttgcgccatc
    14521 accgaagtcg aaatcgacgg tgttcagcat gagtacagcg caatcgaagg catccaagaa
    14581 gacgtaattg aaattctatt gaatttaaaa aacgtcgcta ttgtgatgca cggtaaagat
    14641 cacgccgttg taactctgtc caaaaaaggc ccaggtattg tcactgcagg tgatatcacg
    14701 gtggatcaaa gcgttgagat taaaaacccc gaccttttga ttgctcatat taccggtgat
    14761 gttgccctca acatgcgcct gactatcacc cgtggccgcg gttaccaacc tgctgatgcc
    14821 cgtgagagtt ctgacgacga gactcgcacc attggccgtt tgcacctgga tgcctcctac
    14881 agccctgtgc gccgcctggc gtattctgtg gaaagtgcgc gggttgaaca gcgtacagac
    14941 ttggataagt tggttatcga tttagagacc aatggcacca tcgacccgga agaagccatc
    15001 cgtcgcgcag ctactattct gcaacaacag cttgcagtgt ttgtggattt agaaggcgag
    15061 aaagaatctg aacctgagaa gaaagaagat ctgattgatc ctattctgct tcgcccagtg
    15121 gatgatttgg agcttacggt tcgctcggct aactgtctta aagcagagaa tatttactac
    15181 attggcgatt tgattcagcg cactgaagtt gagttgttga aaacgccaaa cctgggtaaa
    15241 aaatcactca ctgaaattaa agacatc
//
LOCUS       DAOQGS010000019        15171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1804_length_15171_cov_4.430537, whole genome shotgun sequence.
ACCESSION   DAOQGS010000019 DAOQGS010000000
VERSION     DAOQGS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1804_length_15171_cov_4.430537"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..818
                     /locus_tag="PKZ52_01475"
     CDS             198..818
                     /locus_tag="PKZ52_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58462.1"
                     /translation="MGFVWLILSALLLLLAAACLLNFPYLPLLFGLQGAAAHSLRGRI
                     LAKGLSVFPVLALTCVGLGWFLSPLAFLLPLIWLALIWILRPRSEPELTTRFANSEEN
                     RALLARTLSYELGQLAPAEAAGHYLLCTLQCPTEAIAQHICNLLAHLPQHSAPEIKPF
                     AEDTLCQLRIRLNSLEEAAVAALADEINQCAWGNQSIVSSISILRD"
     gene            824..1552
                     /locus_tag="PKZ52_01480"
     CDS             824..1552
                     /locus_tag="PKZ52_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58463.1"
                     /translation="MNTPQTPPLSLEGFYLYTHVSGLALQAFHIQMAQFQAAEEAGAA
                     PALPDAASLGAFLQNGYAAIDELSADIAAKLAQIPAAQLAELISQLPADLPALTRPLN
                     GAQFYDWLLFVLEQPASAAIYQLHHLFNAGPLLDEDPAETIREAIAFDDPALLDASAS
                     IELEHVQLAIQANSLNVLPQLLSASELTDEDSLNLLALALTFDGAMPTLLAALKPSAS
                     LLARLKTLHPDATNPALINYEAQL"
     gene            1549..2025
                     /locus_tag="PKZ52_01485"
     CDS             1549..2025
                     /locus_tag="PKZ52_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58464.1"
                     /translation="MNLWRHIILGVVSFSLSLSACSNPPAKSVEGFVVDGVNEYAFDF
                     AEQQPGAMISFHSKDFQKCTLSFAAKDFGEKFKASAQRLGEGNLDILCVNDEGNFILS
                     PSEPTSVAMEVMAVRPAVQVRVDFNLYNPRKKTWRKAEDIILNLSASQSKRLANNK"
     gene            complement(2080..3000)
                     /locus_tag="PKZ52_01490"
     CDS             complement(2080..3000)
                     /locus_tag="PKZ52_01490"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNG58465.1"
                     /translation="MQKILDTIYTTLSARTDRGQVASYIPELASVNPNQFGICVALAD
                     GRVFSAGDADVAFSIQSISKVFTLTLALGKLGDQVWQHVGREPSGDPFNSIVQLEKEQ
                     GKPRNPFINAGAIAICDAILMGHKPKEALGEIVQFVRFLADDTDIVFDAAVARSEAQT
                     GFRNASLANFMAAFGRIKHPVDWVLGTYFHHCAIAMSCRQLAQAGLFLVGDGANPVSG
                     VRVISAQRARRINSLMLMCGHYDGSGEFAYRVGLPGKSGVGGGILAIAPGKGAIAVWS
                     PGLDTIGNSKLGTQALELLVQHTGWSIFGH"
     gene            3611..4309
                     /gene="pnuC"
                     /locus_tag="PKZ52_01495"
     CDS             3611..4309
                     /gene="pnuC"
                     /locus_tag="PKZ52_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNG58466.1"
                     /translation="MPGILSLRSWSVFELIWIVLFVSAGTLVTIFTKDSLLNYAILLS
                     GIFCVVLAAKGNIWTYAFGLVNSVGYAYVSFTNGLYGEMALNILFFVPTNILGFLMWQ
                     RHQLKDSVAMRGLERAQFSYILLGCVALIAVLGFALSHIKTQNTPYINGISTTLSIVA
                     TILMMLRYKEQWVLYILLNLVTIVMWLIRLLNGSADGTIMILMWGAFLINAVYGYFIW
                     RNGAAVMPKAEPSI"
     gene            4306..5319
                     /locus_tag="PKZ52_01500"
     CDS             4306..5319
                     /locus_tag="PKZ52_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG58467.1"
                     /translation="MKTCGLTLGKYAPFHKGHQFVFDTALAEVDELVVLIYATDVIDI
                     PLSVRSRWIRQLYPNVSVIECWDGPKGYSTERAYEIAEENYIIKMLAGKEITHFYSSE
                     FYGDHVSRALGAVDRRVDTARCTVPISATQIRNAPFDMRALVDTIVYFDMLIKVVFVG
                     AMSTGKSTLVEALAKRYQTSFAAEYGREYWTEHQVNRRIALHEFDLIAKGHMEREKLA
                     ARDANKYLFVDTNAVTTFMYCQDYHGEVTPYLQRTALENPQRYDLWFLCEDDIPYDDT
                     WDRSGPQKRSVFQQKIIADLLERKIPFIRLAGSLDERINTVDRVLTRFGQYSNYFGEK
                     QLL"
     gene            complement(5508..6272)
                     /locus_tag="PKZ52_01505"
     CDS             complement(5508..6272)
                     /locus_tag="PKZ52_01505"
                     /inference="COORDINATES: protein motif:HMM:NF022784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HNG58468.1"
                     /translation="MGRSARNLLVIFIACVGAAAICWWQVEPAQAPKKTNKVQAEHYQ
                     DFWLWPGNSIPTNIPRINNLYILQGEFLGKGSQNNLRLTGILPSRITAKKIWLVFRVE
                     QALWSDAITGAIIHRIKMWEYQGNTVAGIQIDFDARSTALADYGNFLQLVRQELPAHY
                     QLSVTGLLDWSTGAYRSGLANISQTVDEVIFQTYQGRSTIPDFASYLQPISTLKQPFK
                     IGLIEGGLWQPQLPQVKALHHNPYFLGFVVFLNNRD"
     gene            complement(6242..8416)
                     /locus_tag="PKZ52_01510"
     CDS             complement(6242..8416)
                     /locus_tag="PKZ52_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58469.1"
                     /translation="MKLGLISFILFSTCAATTLPSFASSDSYDRSLLAHEPDPELTQM
                     GAQIGTAYLHPWNDTRINLSLLLQDQRPQATPTQSTQMPAFDDSLAPKSVGFYIEEQN
                     SSEAAHSAAVQTQLQRLKIPAEAIIQAQSRLNNWREGRCVSDSWSSLQQFLSQLELAG
                     LKEEETHALGLARLALAGSCPTSKAAPIEIELSTPAAKEFALYIKAAAAFYAGSFTDA
                     GEIFKSLSDAKAPWLHETAHYMLGRVYLNIAQQGAADSWGLFAKDAINTVHLSSASQA
                     FNTYLKRYPEGIYAASAKGLARKIAWLGQNQVELASMYGQEFKQSILNASYEGTISLI
                     NEIETHHWFLSNNNPQTASIFTREPNWRVPEFAAVSVIARQRIFDSSAAEKEQVPPFA
                     VSPDVIKSSLAEFKKNGQEKLGQYLLLSHQFFVQRNHTAVLEATTNIAITDKLTNVDF
                     SSLYLRGLALERTQQKDKAYALWKALASNSKNAGARIQSQIAFIIHLAENDKLIEAFA
                     ENSLITYAPIRKPAITYSANAQVLEYIMQAKHTSTEEKREALFQLVRKNLLHQQYNEA
                     LRIIKLYPLENYALGDTYNQDHTLHASATKLADNLQQYLDNPKDLMALVNLAGRYQNH
                     HITDRTQASLAPGRVGSFTAKWAGEIKSPLQVFQQVIADKNASAEVKAKALSGAINCF
                     RRIGANRCDAQDIPIAQRKAWFRTLKKTYGNSEWAEAQEIYW"
     gene            complement(8496..9200)
                     /locus_tag="PKZ52_01515"
     CDS             complement(8496..9200)
                     /locus_tag="PKZ52_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58470.1"
                     /translation="MRLKPLFYCLFLLATPCLADDQTEEVVVTGIRASQDEGMEIPAI
                     TLKKPADYFVQTVKLINDSRAPDLRKKEILDTISHIISASGKNKGIEVSYGKGFLEPV
                     KMDDETLQIIEDSKKSDTSKIDLAIKKANVANTPAKDQLNVLKKFIKDLKPVGRTLLE
                     PNDDIAVSIVNPERYRTEILAKIAEDTNKTKQALGAAGNCSTIVRGIQHRVEWDRISV
                     AEVMLFIRYENEVECK"
     gene            complement(9459..9956)
                     /locus_tag="PKZ52_01520"
     CDS             complement(9459..9956)
                     /locus_tag="PKZ52_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG58471.1"
                     /translation="MKIKHPKNPKRFMLILSAIVLLHLVVIYGLSSIQPKVDNSPKLV
                     YTSIYTDPNLTPTTFYIALPEAPCVKLQENAPAVTDELPKCLELRKAPELAAQLNQEA
                     YVQMDFTVDNTGKVIDPKVTKSCGNIEVDKAAQKHISETWQFAPCPPDDADGCKRSIK
                     FRWGL"
     gene            complement(10210..11037)
                     /locus_tag="PKZ52_01525"
     CDS             complement(10210..11037)
                     /locus_tag="PKZ52_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58472.1"
                     /translation="MRIFSKIKKALNLKDQELFRRWPASELEEKKFYIQNKPPEEFTT
                     EDRYLAAEWIFQRYLPEGDEPTPEKWTTTISNIRQKIDNNIHKKSAGELFKTAYAPAT
                     PYTLSKEDFLREIVPAFSKFNLLPDPYLKDGQDREIWESLFEFTQPHSNRHVEAKDTL
                     MHFCDPYLAAKLVQDLARTHTALRKAQIDGSKNVELVCTGKQCKACLAMNGKKFDVEE
                     LLNSFRGGNPLFPHQLVLDEEASWCTAPYFSPEIVLGADDNSDFHAWLIKHFETKNK"
     gene            complement(11082..11309)
                     /locus_tag="PKZ52_01530"
     CDS             complement(11082..11309)
                     /locus_tag="PKZ52_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58473.1"
                     /translation="MTGISVEFIGGTLDEQTRTILHKEELSILGYRLQIEVQPKGDEP
                     AFCIAVPIEWTSEQGHKAILKKILTKNPRNQ"
     gene            complement(12233..13159)
                     /locus_tag="PKZ52_01535"
     CDS             complement(12233..13159)
                     /locus_tag="PKZ52_01535"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNG58474.1"
                     /translation="MSFADRDGVIWFDGELIPWRDAKVHVLTHTLHYGMGVFEGVRAY
                     ATAQHGTAVFRLKEHTDRLFRSAHIMRMAMPYTKEQVNEAHKQVIRENNLTEAYLRPM
                     VFYGSEGMGLRADNLKAHVIVAAWNWPSYMSPEAKEAGIKVRTSSYTRHHVNISMCKA
                     KANGHYINSMLALREALDCGCEEALLLDAEGYVAEGSGENFFLVRDGVIYTPELTSCL
                     DGITRNTIFHLAAECGYTIKEKRITRDEVYIADEAFFTGTAAEVLPIRELDGRIIGEG
                     KRGPVTTRLQAMYFDAVKGKLENHFEWLAPTK"
     gene            complement(13272..>15171)
                     /gene="glnE"
                     /locus_tag="PKZ52_01540"
     CDS             complement(13272..>15171)
                     /gene="glnE"
                     /locus_tag="PKZ52_01540"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNG58475.1"
                     /translation="GIREIEFIAQAFQLLRGGREQPLQARSLIPVLNYLSEKGALPQK
                     IAEDLIAAYWFLRNTEHALQALEDRQTQALPTSPTEQARLAWVLGFAHWEAFYAALEQ
                     HRDCVRREFAAVVAAPNEAEEQTGDPLAAYQSFWLGLLGADDNLLHQLAAWGFSPEAD
                     ALGALRSLRQSPKILAMHAQSRERLDVFIPKLLRALRQAQQPQQALTRLLPFVEAVAR
                     RSAYLLLLIENPQALDQLVKLCDGSSWIAERLTKHPALLDELINPASLYHIPTQIELA
                     QELRQHMLRIDLTDLEAQMEGLRYFRWAQALKIAACEVTGVLPLMRVSDCLTYLAEVI
                     INYTYNLAKDEMISKHGRPTNLPESGELPFIIIAYGKLGGLELAHGSDLDLVFLHNSD
                     PNSQTDGAKPLEVSVFYPRLAQKMIHILATKTASGELYEVDVRLRPSGNSGPLVTSLS
                     AFEKYQRESAWTWEHQALVRARPVAGEPELQQAFVDLRLALLCQARDITKLTEDVRSM
                     REKMRTQLGSKKTDQAAGLFNLKQDAGGIVDIEFMVQYLALAWAHADPSLVRYTDNIR
                     ILGSLETTGRLAAQQAHQLINAYKEYRTLGHKLALQQAPTITQRQPLAEPIAQVCTLW
                     QQVIGPQE"
BASE COUNT         3514 a         3523 c         3891 g         4243 t
ORIGIN      
        1 gctacgcagt ggaaatttcc cacgatattt tggttaaact gctgcgtaaa ctcaaagtgg
       61 aattggccct ggcggccaaa atggccctgg cagcggccgg taaaataggc cgttccaccg
      121 agtttaaaat cattctggcc gccagcggcg ccgggtcttt ggggcttttg ttgttataag
      181 tgtaagcggg tgcgcgtatg ggttttgtgt ggttgatttt aagtgcgttg ttattgctgt
      241 tagccgcagc ctgcctgctc aatttcccct acctacccct cctgtttggc ctgcagggcg
      301 cggcagccca cagcctgcgg gggcgcatct tagccaaggg cttaagcgtt tttccggtgc
      361 tggccttaac ctgtgtgggc ctgggttggt ttttaagccc gctggcattt ttgctgccct
      421 taatctggct ggcattaatt tggatattgc gcccgcgcag cgagcctgaa cttacaacac
      481 gctttgccaa tagcgaggaa aatcgcgctt tattggcgcg cacacttagc tatgaattgg
      541 gccagttggc ccccgctgag gccgctgggc actatttatt gtgcaccctg cagtgcccca
      601 ccgaggcaat tgcccagcat atttgcaacc tgctggccca cctgccgcag catagcgcgc
      661 cggaaattaa accctttgct gaggatacgc tgtgccagct gcgcatcagg cttaacagcc
      721 ttgaggaggc cgctgtggcg gcactggcgg atgaaataaa ccagtgtgcc tggggcaatc
      781 aatccatagt atcgtccata tcgattttgc gagattaata gccatgaata caccccaaac
      841 cccgccactg agcctggagg gtttttacct atacacccac gtgagcggtt tggcgctaca
      901 ggcgtttcac atccaaatgg cgcagtttca agccgctgag gaagccggtg ctgcgcctgc
      961 gctgccggat gcggcaagcc tgggggcatt tttacaaaat ggctacgcgg ctatagacga
     1021 attaagcgcg gatattgcgg ccaaattagc gcaaatcccc gccgcgcaat tggccgaatt
     1081 aatttcccag ctgcccgccg acctgcccgc gcttacccgc ccattaaacg gcgcgcagtt
     1141 ttacgattgg ctgctgtttg tgttggagca acccgccagc gccgccattt accaattaca
     1201 ccacctattt aatgccggcc ccctgctgga tgaagaccca gccgaaacca ttcgcgaggc
     1261 catagccttt gacgacccag ctctgttgga cgcgagcgcc agcattgaac ttgagcatgt
     1321 gcaattagca attcaagcga actcgttaaa cgtgttacca cagttattat cggccagcga
     1381 attaacggat gaggactcat taaatctgct cgcgcttgcg ctcacctttg atggtgccat
     1441 gcctacatta ctggcggccc tcaaaccctc ggcgtcgcta ctggcgcgtt taaaaacctt
     1501 gcatcctgat gcaaccaacc ccgctttaat aaattatgag gcgcaactat gaacctttgg
     1561 cgccacatta ttttaggagt tgtaagtttt agtttgagct taagcgcctg cagcaaccca
     1621 ccggccaaat ctgtggaggg atttgtggtg gatggcgtga atgaatatgc ttttgatttt
     1681 gccgaacaac aacctggcgc catgatcagt tttcactcca aggattttca aaaatgcacc
     1741 ctaagttttg ccgccaaaga ttttggtgag aaattcaaag ccagcgccca gcgcttaggc
     1801 gaaggcaatc ttgatattct atgtgtaaac gacgaaggca attttatctt aagcccctcc
     1861 gagcccacct cggttgcaat ggaggtaatg gctgtgcgac ctgctgtgca ggtgcgcgtg
     1921 gattttaatc tttataatcc gcgtaaaaaa acctggcgca aggcggagga tataatcctg
     1981 aatttaagcg catcccagag taaacggttg gcaaataata aatagctgtt ttagttgatt
     2041 ggttgcaggt gcttcaccgc atatattttt cgcagtttgt taatggccga aaatagacca
     2101 gccggtgtgc tgtaccaaca attccagcgc ttgggtgcct aatttgctgt tgccaatggt
     2161 atcaagcccg ggcgaccaca cggctattgc ccctttgccc ggtgcaatag ctaatatgcc
     2221 tccgcccaca ccgcttttac ccggcaggcc aacccggtag gcaaattccc ctgagccgtc
     2281 atagtggccg cacatcaaca ttaatgaatt aatgcgccgc gctcgctggg cggatatcac
     2341 ccgcacaccg cttacaggat tggcgccatc acctactaaa aataaaccgg cctgggctaa
     2401 ttggcggcag ctcatggcaa tggcgcaatg gtgaaaataa gtgcccaaca cccaatctac
     2461 tgggtgttta atgcggccaa acgccgccat aaaattcgct aaggaagcgt tgcgaaagcc
     2521 ggtctgcgct tccgagcgcg ccaccgccgc atcaaaaact atatcggtat catccgccaa
     2581 aaaccgcaca aattgcacaa tttcccccaa tgcctctttg ggtttatggc ccattaaaat
     2641 ggcatcacaa atagctatgg caccggcatt aataaagggg ttgcgcggtt tgccctgttc
     2701 tttttctaat tgcacaatag aattaaacgg gtcaccggag ggctcgcgcc ccacatgctg
     2761 ccacacctgg tcccccaatt tacccagggc taatgtgagg gtaaaaacct tggaaatact
     2821 ctgaatcgaa aacgccacat ccgcatcacc tgcactaaat acgcgcccat ccgccaaagc
     2881 tacacagatg ccgaattggt tggggtttac gctggctaat tcggggatgt agctagccac
     2941 ctggccgcga tcggtacggg cggatagtgt tgtgtagatg gtatcgagaa ttttttgcat
     3001 ggcatgctcg cggcttatgt tttggcagag ttttacgctt tgtatcctcg ggcaagctaa
     3061 gtcaacgctt tatgaggtga gaaattctgc gcactccggg atggttttga ggtaaaaact
     3121 taaggcgtgt taaacaggca ttttgggtac tctgcctcca acctgcaagt gttaaatagg
     3181 cataggtatt taggctggaa attaatacaa agcctgatta ttcctgtata aaatgggccg
     3241 ctgtccccaa aaaaatatgt gttcgcgagc agtaaataaa tgattagagt catttaaaca
     3301 tgtgttcgcg tggtgaagtc cagcagggtg cccagcagga actattttgt ggagttgtat
     3361 ttccgtaaac tagttggcat gataattgtg gctgcaagct gagcttacta taaatgccaa
     3421 acctaggttc agtcgttgtg gcgcggcggc aatttacggt ccgaatatgg cgtcttatca
     3481 acgggtttgc tcgtgtaata ctcggcggaa gaataattaa taattaagaa cttaaagcgt
     3541 cgatgggttt gggtgctaaa ccacacacct gtggacgcat aaaattaaat atcactaagg
     3601 agcaagtagc ttgcccggta tcttaagtct gcgaagctgg tccgtttttg agcttatttg
     3661 gattgttctt tttgtgtctg ccggcacgct agtgacaatt ttcaccaagg actctctgct
     3721 gaattacgca atcctgttga gcggtatttt ttgcgtggta ctcgctgcaa aaggcaatat
     3781 ttggacttac gcgttcggcc tagttaatag tgtgggctat gcatatgtct cattcaccaa
     3841 tgggctttac ggtgaaatgg cgcttaatat actgttcttt gtgccgacga atattcttgg
     3901 ttttttgatg tggcagcgac accagctaaa agactctgtt gcaatgcgag ggcttgagcg
     3961 tgcgcaattt tcctatatcc tgctgggctg tgtagcctta attgctgttt taggtttcgc
     4021 cctctcgcac ataaaaaccc agaatacgcc ctatattaat ggcatttcaa cgaccctttc
     4081 gattgtggca actatattaa tgatgttgcg ctacaaagag cagtgggtac tctatatcct
     4141 gttaaaccta gtgacgattg ttatgtggtt gatacggctg ctcaacggca gtgctgacgg
     4201 gacgattatg attctaatgt ggggtgcgtt tctaattaat gcagtttacg gctattttat
     4261 ctggcgaaat ggtgcagctg taatgcctaa agcggagcct tcaatatgaa gacttgcggg
     4321 ctgacgctcg gtaaatatgc accctttcac aaagggcatc agtttgtttt tgataccgca
     4381 cttgcagagg ttgacgagct ggtggtgctg atttacgcca cggacgtaat tgatataccc
     4441 cttagtgtgc gcagtcgatg gattcgccag ctgtatccaa atgtgagcgt gattgagtgc
     4501 tgggatgggc ctaagggtta ttctacggaa agagcctatg aaatagcaga agaaaattac
     4561 atcataaaaa tgctggcagg caaagaaatt actcatttct attcaagtga gttttacggc
     4621 gaccacgtga gtcgggcgct cggcgcagta gatcgacgtg ttgatacggc tcgttgtacc
     4681 gtgcctatat cggcaacaca aatacgaaat gcgccgtttg atatgcgcgc gctggtcgac
     4741 accattgtgt atttcgacat gctgataaag gtggtttttg ttggcgccat gtccaccgga
     4801 aagtccaccc ttgttgaggc tttagccaag cgctatcaaa caagttttgc tgctgaatac
     4861 ggccgagagt actggactga gcatcaggtg aatcgcagaa tcgccctgca tgagtttgac
     4921 ctcatcgcca aagggcatat ggaacgggag aagctggcgg ccagagatgc taataaatat
     4981 ttatttgtcg ataccaacgc tgtgacaaca tttatgtatt gccaggacta ccacggcgag
     5041 gtaacgcctt atctgcagcg cacagcgctg gaaaacccgc agcgctatga cctttggttc
     5101 ctgtgcgagg atgatattcc ctacgatgat acttgggaca gaagcggccc gcaaaaaagg
     5161 tctgtatttc aacaaaaaat tattgcagat ttgctcgagc gaaaaattcc ctttataagg
     5221 cttgccggtt cattggatga gcgtattaat acggtagatc gggttcttac acggtttggt
     5281 cagtacagca attactttgg ggaaaagcaa ttactctgat cgagtaagga tgaataaaac
     5341 tagcgctaca atgtgccaca ggagtatcgg cgtgcctttg tttttgagat ggaattcggt
     5401 gagcttttct atctcccgat tttcgtgtaa tttcaataaa ttatttggtt gcatcgcatt
     5461 aatataatta attatagtgc ttcattctat aatttattgg tatttattta atcgcgattg
     5521 tttagaaaaa ccacgaagcc taaaaaatat ggattgtggt gcagagcttt tacctggggc
     5581 agttgtggct gccatagccc gccttctatt agcccaattt taaatggctg ttttaatgtc
     5641 gatataggct gcagatagct agcgaaatcg ggaattgtgg agcgcccttg gtaggtctgg
     5701 aatattactt catctactgt ttggctaata ttggcgaggc ctgaacggta agcgccggtg
     5761 gaccaatcca gcagccctgt aacgcttaac tgatagtgag ccggtagttc ttgccgcacc
     5821 agctgtaaaa aattgccata atctgcgagc gcagtacttc tggcatcaaa gtcgatttgg
     5881 ataccggcga cggtgttgcc ctgatattcc cacatcttaa ttcggtgaat tattgcccct
     5941 gtaatagcgt cactccatag ggcctgttcc actcgaaata ccaaccagat ctttttagca
     6001 gtaatccggc tgggcagtat tcctgtaagg cgcagattat tctggctgcc cttgcctaaa
     6061 aattccccct gcagaatata taaattattg attcgtggga tattggttgg gattgaattg
     6121 cctggccaca accaaaaatc ctgatagtgt tctgcctgca ctttattggt ttttttagga
     6181 gcttgtgctg gctccacctg ccaccagcaa atggctgcgg cgccaacaca ggcaataaaa
     6241 attaccaata aatttcttgc gcttcggccc attcgctatt tccgtaggtt tttttcaggg
     6301 ttctaaacca agctttgcgc tgtgcaatag ggatgtcctg agcgtcgcaa cggtttgcgc
     6361 caatccggcg aaaacaatta atagcgccgc taagggcctt agccttaacc tctgccgagg
     6421 catttttatc tgcaatcacc tgttgaaata cctgtaaggg tgatttgatt tcgcctgccc
     6481 atttggctgt aaaactgccc acccgccctg gagcgaggct cgcttgcgtt cgatcggtaa
     6541 tgtgatgatt ctgatagcgg cccgccaaat tcaccagagc cattaaatcc ttagggttgt
     6601 ccaggtactg ctgcaggttg tctgcgagtt ttgttgcgct ggcatgtagc gtatggtcct
     6661 ggttgtaagt atcacctaag gcgtaatttt ctaaggggta taatttaata atacgaagtg
     6721 cctcgttgta ttgctgatgc agcaagttct tgcgcacgag ttggaataac gcctcacgtt
     6781 tttcttcggt tgatgtgtgt ttggcttgca ttatatattc gagtacctgt gcattagccg
     6841 agtaggtaat ggctggcttg cgaatcgggg cgtaggtgat aagcgaattt tcagcgaatg
     6901 cttctatgag cttatcgttt tctgccaaat ggataataaa cgcgatttgg ctttgaatgc
     6961 gggcacctgc atttttagag ttgcttgcca acgctttcca gagtgcatag gctttgtctt
     7021 tttgctgagt tctttccagt gccaagccgc gtagataaag gctactaaaa tctacattag
     7081 taagcttgtc agttatcgcg atatttgtag ttgcctctag aacagcagtg tggttacgct
     7141 gcacaaaaaa ctggtgactg agtaataaat attgtcccaa tttttcttgc ccgttttttt
     7201 taaattcagc taacgaggac ttaatcacat caggggatac ggcaaaaggc ggaacctgtt
     7261 ctttttccgc tgcgctggag tcaaaaatac gttgccgggc tataactgac acagcggcaa
     7321 attccggaac gcgccagttg ggctcgcggg tgaaaatgct tgcggtttgg gggttattat
     7381 ttgataaaaa ccagtggtga gtttcgatct cattaattaa tgaaattgtc ccttcatagg
     7441 atgcgtttaa gattgattgc ttaaattctt ggccgtacat actggcaagc tccacctggt
     7501 tctgccccag ccaagcaatt ttgcgggcta agcctttggc ggaagcggcg taaatgcctt
     7561 ctgggtaacg ttttaaataa gtgttaaagg cttggctggc gctgcttaaa tgcacggtat
     7621 ttatggcatc tttagcaaac aggccccagc tatcggcggc accttgttga gcaatattta
     7681 aatagacacg gcctaacatg tagtgcgccg tttcgtgcaa ccagggtgcc ttggcgtcgc
     7741 tgagcgactt aaatatttcg ccagcatcgg taaagctccc ggcgtaaaaa gctgcggctg
     7801 cttttatata aagtgcgaat tcttttgctg ccggagtgga taattcaatt tcaatggggg
     7861 cggctttgct agttgggcag gagcccgcaa gcgctaatcg ggccaagcct aatgcgtgtg
     7921 tttcctcttc tttaagtcct gccaattcca attgactgag aaattgttgc aagctgctcc
     7981 aggaatcaga aacacaccgc ccttcacgcc agttattcaa acggctttgc gcctgtatta
     8041 tggcttcagc ggggattttt aagcgttgca gttgagtttg cacggcagcc gaatgcgcag
     8101 cctcacttga attctgttcc tcgatataaa acccaacgct ctttggcgcc aaactgtcgt
     8161 caaaggcagg catttgggtg ctctgtgtgg gtgtggcttg cggtctttgg tcttgcaata
     8221 gcagacttag attaatgcgt gtgtcattcc aggggtgcaa ataggcggtg ccaatttgcg
     8281 cgcccatttg ggtgagttct gggtctggct cgtgggccag cagggacctg tcataactat
     8341 cgctggaggc gaaacttggc agtgtagtag cggcacaagt gctaaacaga ataaatgaaa
     8401 ttagtccgag tttcattggg gagtccttaa tgggtaaatc gcggcggtat tagtatcaga
     8461 agggtgaagc ggcgcggcac accaattaaa acccgctatt tacattccac ttcattttca
     8521 tagcgaataa acaacattac ctccgccacg gaaattctgt cccactccac cctatgttgt
     8581 atgccacgca ctatagtgct gcagttgccc gccgcgccga gcgcttgttt ggttttgttg
     8641 gtatcttcag ctatttttgc caaaatctct gtgcggtatc tttctgggtt aacgatactt
     8701 accgctatat cgtcattcgg ttctaacaga gtgcggccga cgggttttaa atcttttata
     8761 aattttttca gcacattaag ctgatcttta gccggagtat tggcaacgtt tgcctttttt
     8821 attgccaaat caattttact ggtgtcactt tttttgctgt cttcgatgat ttgcagggtt
     8881 tcatcgtcca ttttcaccgg ctccaaaaac cccttgccgt aggatacttc aataccttta
     8941 tttttaccgc tggcgcttat gatatggctg atggtgtcga gaatttcttt tttacgcagg
     9001 tccggtgccc ggctgtcgtt aattagtttt accgtctgta caaaataatc ggcaggcttt
     9061 ttaagtgtta tagccgggat ttccattccc tcgtcctggc tcgcgcggat gccggtgaca
     9121 accacttctt cggtttgatc atcagctaaa cacggggtgg caagtaaaaa caggcaatag
     9181 aacaggggtt ttaatctcat gaggtaatcc gtctgttggg ttgagggtga gtgggctatt
     9241 gggtgcccag cgattgtagc gtaatttttt gaccgtgtag cctggatgca gcgtagcgta
     9301 atccgggtgg caatattaca aatataacag cggtagtgcc aacgcattaa cccggattac
     9361 gctgcgctgc atccaggcta ctgttggttg gaatttgatg cacgaaaaat ccaacaaaaa
     9421 atttgcaagt tgtgttcaaa attcgcttgg acggaaattt ataagcccca gcgaaatttt
     9481 atactgcgtt tgcagccatc ggcatcatct ggagggcagg gggcaaattg ccaggtttcg
     9541 ctaatatgct tttgcgccgc tttatctact tctatattcc cgcaggattt tgtcaccttg
     9601 ggatctatca ctttacccgt attatcgacg gtaaaatcca tctgcacata cgcctcttga
     9661 tttaattgcg ctgccaattc tggcgctttg cgcaattcca aacacttggg taattcatcg
     9721 gttactgcag gcgcattttc ttgcagtttt acacaggggg cttctggcag ggcgatataa
     9781 aaggtggtgg gggttaaatt cgggtcggtg tatatggatg tataaaccaa cttgggtgag
     9841 ttatcgactt ttggctgtat acttgataag ccatagatca ccaccaggtg aaggaggaca
     9901 atggcagata aaatgagcat aaaccgtttg gggtttttgg ggtgttttat tttcatgggt
     9961 tcgtcttttg tttgatctgg taggctaggt tcaatttagt tgtttgtcga aaattctatt
    10021 ttaaggatgt tcggaacaca tgtagggtgt gcaagctagc ttgcctacca attcagtttt
    10081 cagttcagcc ttgcttgggt gttgcgataa tttataacgt gcccaaaagt ggtgggcaac
    10141 aagttgccca ccctacgcgc tcatgtttat gtgagtgaat tgaaattttt ttcgattaaa
    10201 atatgcattt tatttattct ttgtttcaaa gtgtttaatt agccaagcat gaaaatctga
    10261 attgtcatcc gctccaagta caatctctgg tgagaaatat ggtgcagtgc accagctggc
    10321 ttcctcgtcg agaactaatt ggtgagggaa taaaggattc cctcctcgaa aggaatttag
    10381 cagttcttca acatcaaatt tttttccatt catggctaag caggctttgc attgcttgcc
    10441 tgtgcaaact aactccacat tttttgagcc atcaatctgc gctttacgca gtgcggtatg
    10501 ggttcgggcc aaatcctgaa ctagtttcgc cgcaaggtag gggtcacaga agtgcatcaa
    10561 tgtatctttt gcttcaacat gcctattaga atgtggttga gtgaattcaa ataacgactc
    10621 ccaaatctct ctgtcctggc catccttcaa ataaggatca ggcaatagat taaatttcga
    10681 aaatgcaggg actatttctc tcagaaaatc ctctttgctt agagtgtatg gcgttgctgg
    10741 tgcatatgca gttttgaaaa gttctccagc agattttttg tgtatattgt tgtcaatctt
    10801 ctgccgaatg ttacttatag tcgttgtcca tttttcaggc gttggttcat caccttctgg
    10861 taaatatcgc tgaaatatcc attcggcagc cagatagcga tcttcggtcg tgaattcttc
    10921 gggaggctta ttctgtatat aaaatttctt ttcttcaagc tcagaagccg gccatcgccg
    10981 aaaaagctct tgatctttta ggttgagagc tttttttatt ttgctgaaaa ttctcattgg
    11041 ggcccgccaa atgatttttt ggtttctaca ataatgatta attattggtt tcgagggttt
    11101 ttagtgagaa ttttttttaa aattgcttta tgcccttgct ccgaagtcca ttcgatgggg
    11161 acggctatgc agaaagcagg ctcgtcacct ttgggttgca cctcgatttg cagtcgataa
    11221 ccaaggatag acaactcttc tttatggaga atggtgcgag tctgttcatc tagagtgcca
    11281 ccgataaatt caacactgat acctgtcatt tgattcacct ttgtatgttt ttcaaatttt
    11341 cgcctttgag ggctgtgtac aacgtttatt tttagcttct tccacttgcc gaatttttgg
    11401 gtgcagctat tgggtcagtt ggctgggcga caattcccaa tacaataaat tattttgcta
    11461 gccttgagtt gctttaaatg tcatagttgc gacaattttt tatcgaagtt attttcatgt
    11521 tgggcattac tgcccaacct acttcttttt ctacatctaa atattcacgc tgcccgaggt
    11581 gaaacctctc tgcgaatctc cgtcccacgc tccagcttcg ccacctttaa atcataagcc
    11641 gtctgtaagt tcagccaaga ctggcctcct cgccaaaata ccgggccagc cgcaaagcgg
    11701 tgtcggccgt aatggagcgg cgttgcttgg tgactatatg cagacaggtg gcgggcgcgc
    11761 gtagggcctg ggcgagggcg tttacgctta attctagggg gattaattac tcctcccgca
    11821 gcatttcgcc tgggtggatg gggtgcattt tgttggatgc cataagaatc cttgctattg
    11881 agatggtgct gaattcaaaa agtgagttga tatgtaccta taccggcttt tgcttaaatt
    11941 gtgctgcccc tatgctctct gctcaaaacc cccacccagc ctcccccttg aaaaggggga
    12001 ggagctaaaa accttcgcac caaggcatta taagagcgct ccgggctttt ttgctccctc
    12061 ccctttttaa ggggagggtt ggggtggggt ttttggggag tgcaggatca ggtaaataaa
    12121 ggtacacaat gtttaaatta atgatcttgt cttggatagg attcgcaagc ttagtattta
    12181 tttaactata aaaaagccgc gttaaacgcg gctctcaaac aatctaacta aattatttcg
    12241 tcggcgcgag ccattcaaaa tgattttcca gcttcccctt caccgcatca aaatacatcg
    12301 cctgcaagcg cgtagtcacc ggcccgcgtt tgccttcacc aataatgcgc ccatccaatt
    12361 cgcgaatcgg taaaacttcc gccgctgtac cggtaaaaaa cgcttcatcg gcaatataaa
    12421 cttcatcgcg ggtaatacgt ttttctttaa tggtgtaacc acattctgct gccaaatgga
    12481 aaatagtatt gcgggtgatg ccgtctaaac agctggtgag ttccggggtg taaataacgc
    12541 cgtcgcgcac taaaaagaag ttttcgccag agccttcggc cacataacct tcggcatcta
    12601 ataacagggc ttcttcgcag ccgcagtcca gggcttcgcg cagagcgagc atggaattga
    12661 tgtagtggcc gttggccttg gccttacaca tggagatatt tacatggtgg cgggtgtaac
    12721 tgctggtgcg cactttaatg cccgcttctt tggcttccgg gctcatgtag gagggccagt
    12781 tccaggcggc cacaatgacg tgggctttta aattatcggc gcgcaggccc ataccttctg
    12841 agccgtaaaa caccatgggg cgcaaatagg cttcggtgag gttgttctcg cggatcactt
    12901 gtttgtgtgc ttcatttacc tgctcctttg tgtagggcat ggccatacgc atgatgtggg
    12961 cggagcggaa caggcggtcg gtgtgttctt ttaaacggaa gacggcggtg ccgtgttggg
    13021 cggtggcgta ggcgcgcacg ccttcgaata cgcccatgcc gtagtgcagt gtgtgggtga
    13081 ggacgtgaac cttagcgtcg cgccagggga ttaattcccc atcaaaccag attacgccgt
    13141 cgcggtcggc aaatgacata gtgaactcca ggaagcctgc ggcttgtttg tgactgtata
    13201 aaaggaacgg taaatacgtg tgttgcttag gtttaggcta ggtggctgga gttgcgtatg
    13261 caagcgggcc tttattcctg cgggccgatc acctgttgcc acagcgtgca tacctgcgcg
    13321 atgggctcgg ccaggggttg ccgttgggtg atggtgggtg cttgctgcaa agccagtttg
    13381 tggcctagcg tccggtactc tttgtaggcg ttgatcagtt ggtgtgcctg ttgggcggcc
    13441 aaccgtccgg tggtttctaa gctgcctagg atgcggatgt tgtccgtgta acgcacaagg
    13501 gatggatcgg cgtgtgccca ggccagtgcc aggtattgaa ccataaattc aatatccacg
    13561 ataccgcccg catcctgctt gaggttgaat aatcccgccg cctggtcggt ttttttgctg
    13621 cccaattgag tgcgcatttt ctcgcgcata ctgcgtacat cttcggtgag tttggttatg
    13681 tcccgcgctt ggcagagcag ggccaggcgt aaatccacaa acgcctgttg cagctctggc
    13741 tcccctgcca ctgggcgggc gcgcaccagg gcttggtgct cccaggtcca ggcggattcc
    13801 cgctggtatt tttcaaaggc gctaaggctg gtcaccaggg ggcctgagtt gccggagggg
    13861 cgcaggcgca cgtctacttc gtagagttcg ccggaggcgg ttttagtggc caggatgtga
    13921 atcatctttt gcgccaggcg cgggtaaaac acgctcacct caaggggctt ggcgccatcg
    13981 gtttggctgt tggggtcgct gttgtgcagg aagactaggt ctaaatccga gccgtgggcc
    14041 agttctaagc cgccgagttt gccgtaggca atgatgataa acggcagttc tccactttcc
    14101 ggcaggttgg tggggcggcc gtgtttactg atcatctcgt ctttggccag gttgtaggtg
    14161 taattgatga tgacttcggc gaggtaggtg aggcagtcgc tcacccgcat caggggcaat
    14221 acgccggtga cttcacaggc ggcaattttc agcgcttggg cccaacgaaa gtagcgcagc
    14281 ccctccattt gcgcttctaa atcggtgagg tcgatgcgca gcatgtgctg gcgcaattcc
    14341 tgggcgagtt caatttgcgt ggggatgtgg taaagggacg ccgggttaat taattcatct
    14401 aacagagccg ggtgtttggt gaggcgctcg gcgatccagc tgctgccatc gcagagtttt
    14461 accagctggt ctagggcctg ggggttttca atgagcaata acaaataggc gctgcggcgg
    14521 gccacggctt ccacaaaggg taacaggcga gtgagggcct gctggggctg ctgggcttgc
    14581 ctgagcgccc gcaataattt cgggataaac acatccagcc gctcccggct ttgggcgtgc
    14641 atggcgagga ttttcgggct ttgccgcaag ctacgcaggg cgcccaaggc atctgcttcg
    14701 gggctaaaac cccaggcggc cagttggtgc agcaggttgt catccgcgcc caacaggcca
    14761 agccagaaac tttggtaagc ggccaaaggg tcgccggttt gttcctccgc ctcattgggg
    14821 gcggccacca cggcggcaaa ctcgcggcgc acacaatccc ggtgttgctc tagggccgca
    14881 taaaacgcct cccagtgagc aaagcccagc acccaagcaa ggcgcgcctg ctcggttggg
    14941 ctggtgggca gggcttgggt ttggcggtct tcaagcgctt gtaaggcgtg ttcggtattg
    15001 cgtaaaaacc aataggcggc aattaaatcc tcggcaattt tttgcggcag ggcgcctttt
    15061 tcacttaaat aattgagcac gggaataagg ctgcgcgctt gcaagggctg ctcccggccg
    15121 ccgcgcaata attgaaacgc ctgggcgata aattcaattt cccgaatacc a
//
LOCUS       DAOQGS010000020        15027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1829_length_15027_cov_4.361007, whole genome shotgun sequence.
ACCESSION   DAOQGS010000020 DAOQGS010000000
VERSION     DAOQGS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1829_length_15027_cov_4.361007"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="infC"
                     /locus_tag="PKZ52_01545"
     CDS             complement(<1..447)
                     /gene="infC"
                     /locus_tag="PKZ52_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNG58476.1"
                     /translation="MREPAAAAKPRSKKAAINEQIEAKQVRLIGANGEQIGIVSIAEA
                     LAAAQAATLDLVEIAVDADPVVCKIMDYGKHLFELKKSKNAAKKKQKQQQIKEMKFRP
                     GTEEGDYQVKLRALIRFLEQGDKAKVSLRFKGRELAHQELGMEMMQR"
     gene            complement(493..2412)
                     /gene="thrS"
                     /locus_tag="PKZ52_01550"
     CDS             complement(493..2412)
                     /gene="thrS"
                     /locus_tag="PKZ52_01550"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNG58477.1"
                     /translation="MITVSLPDGSSRQFPSAVSVFDVAANIGAGLAKATLGGIVNGTE
                     VDASFLIESDASLVIITDKSDQALDLLRHSTAHLLAQAVKQLYPDAQVTIGPVIEDGF
                     YYDFAYSRAFTPDDLIKIEERMQALAAQDIPVVRRVLPRDAAVEYFKGLGEHYKAEII
                     QSIPANEDLSLYRQGDFEDLCRGPHVPSTGKLKFFKLMKVAGAYWRGDAKNAMLTRVY
                     GTAWSTKKDLDAYLHRLEEAEKRDHRKLGKKFDFFHIQEEAPGMVFWHPKGWTIYTAI
                     ETYMRKVQRDNGYQEIKTPQVVDRSLWERSGHWDKFQTGMFTVASEQRDYAIKPMNCP
                     CHIQVFNQGLKSYRDLPLRLAEFGSCHRNEASGTLQGLMRVRSFVQDDAHIFCSENMI
                     QTEVSAFTDLLYQVYADFGFSDVIIRLSTRPEQRVGSDEVWDKAEQALADALNAKQLP
                     FEYLPGEGAFYGPKIEFSLKDCIGRVWQCGTIQVDFSMPARLDAQFVAEDGSRQVPVM
                     LHRAILGSFERFIGILIEHYEGAFPTWLAPEQVAVLNITDNQADFAQTVAKSLQEKGF
                     RAFADLRNEKIGFKIREHTIQKVPYLLILGDQEVASGKVSVRTRTGTDLGTLSLDDFL
                     ALLTNDVANKSRVSH"
     gene            complement(2621..3706)
                     /locus_tag="PKZ52_01555"
     CDS             complement(2621..3706)
                     /locus_tag="PKZ52_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58478.1"
                     /translation="MDIRPRVLSVDNNLSNQKLVSSVLAADAEVYTASSGAQALRMLE
                     SLRPDLILLDIDMPGMDGFQTCRSMRANPDFALTPIVFVSGLTERDEQLTAFQCGGDD
                     FIAKPFDVEILRAKVGALIQRYRRQQFIRNPNADNQGFDHLRRVNHYLLGALKVSTLA
                     KLNDATLEALTALDLKAALYIHKKPELLASTCGSLTDLETQLLSQTKGAQMQALGGRF
                     MLGSAQAAWLIQNMPRAGSNEFARLHDALVIMFDAYSQKVQTLLVKFAQEVSPAPLSA
                     PLPVETLLNQLADLDDATDDAVSECLNQLQLLQKQTSGSLYERTQLAVLDTKLRRLQD
                     DHAKRCREFKLALNKARAATAVLLDSA"
     gene            complement(4201..4644)
                     /locus_tag="PKZ52_01560"
     CDS             complement(4201..4644)
                     /locus_tag="PKZ52_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG58479.1"
                     /translation="MATFRPANQSDCRWLWQVREHPSVAMQSLNPSPIPYIKHCRWFR
                     HLLRSQQRRLDIFIDDISGQRLGYIRAEPYQTGQLLSWAIAPEFHGQGLGKQMLSSWC
                     QAAETTLWAQIKAENSASKAIAAQARFTQIEEKNGIETWLKTLTY"
     gene            complement(4644..6770)
                     /locus_tag="PKZ52_01565"
     CDS             complement(4644..6770)
                     /locus_tag="PKZ52_01565"
                     /inference="COORDINATES: protein motif:HMM:NF040131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNG58480.1"
                     /translation="MVSAQESSPQNPLDQLQDLLRQAELQNLQLQLELRFKKNMAIFK
                     EAAPEIYHQFIHYEPKELRLLYTEDGHLNLVNYTLNNKPVYPGDPSEFIDKQVADFIA
                     LPSISSVNFAKSKIMNDAHIHAPLVNHLIDSYTPFHDSIKVTCNQPIGMFLMTGVGLG
                     YQLPQLLAKLDIYTLCIFDPHKDSFYASLHTIDWGEIIQYFLRPYRMIKLLVGMETQD
                     AMAELRLLTDRIGLHNVVFTFIYRHFGSSKEEEFITTYTKEFHLNATGTGFFDDEQVS
                     FAHTIANLNNQTPIFLHQSDKTTPLPPLLLVGNGPSLDKHIEFLQRNRSNAIVMSCGT
                     ALSSLSKRKIKPDFHVEMERNIDVKDWLEVGTEPEDREGVTLLCLNTCAPDVIKMFSD
                     ACIAKKPNDCGEIIIDDLVEQTCQGTAIRALPLCNPTVTNAGLSFALAMGFTEIYLVG
                     VDLGMSPEGDHHSTLSPYHALAAKTQIKDLSPLENTKTNYFIKGNFVEQVSTSVFLDS
                     TRVNMQILLTYYERTKGGVKVYNSNNGALIKGTIPTPLSDIKDFANLGNKAAIIQELK
                     QTHFIRPKESNSIQKSQVNGYLRNYFEMRKQIGLPKKIANRKVFYERITEIFNLVRQQ
                     RDKNSITTMLLRGSMHGYFTLMLRACLFQKNQHDYERAYEIGRKAYMTFLKQCYAIME
                     STPLKLDDTHDKNIIAARKILGNEKC"
     gene            6893..7180
                     /locus_tag="PKZ52_01570"
     CDS             6893..7180
                     /locus_tag="PKZ52_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebG family protein"
                     /protein_id="HNG58481.1"
                     /translation="MAVIAVWQCDRDGAMFQNKKDADEYDKMLELAESIGQFLEQNVP
                     GLSEAQTESIGLLLARRREALVKACKGRPEALFEPSEEVQVEPFAILPKKS"
     gene            7393..10020
                     /locus_tag="PKZ52_01575"
     CDS             7393..10020
                     /locus_tag="PKZ52_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNG58482.1"
                     /translation="MSEFVQGQRWVVDSEPELGLGIIMSVQARTVEVFFPQVETERLY
                     AKNQAPLTRITYGVDDTIKLKDGSSARVVQVLDNKGFLLYDLGGDRLVPETELAGDIQ
                     LNQPYMRLLTGQLDKSRWFYFRRQLDDALARTWNSRLNGLLGIRANLIPHQLYVAWAA
                     CEREQVRVLLADEVGLGKTLEAGMILTRLLKLERIGRALIVVPDPLQVQWLVELIRRF
                     SLRPELYAGTDHDFSIGQIHLIPQSVLAAQADLLLNAEFDCVIVDEAHHIQPATQNFS
                     VLERLAHLCPHVVLLTATPEQLGFNSHFARLQLLDPAKYTDIERFTQEEKSFAEINQL
                     IRQLPASRAQLVKRFDLDNELNDEDLIAQVLDCHGLGRVMFRNSRTQVKGFPARIAVP
                     HLLTEDTWPARFEWLAQFLKAHKQEKILVICHKQANVLEAETFLWQKHGLDAALFHEG
                     MNLVERDRAAAYFANQEEGAQVLLCSEIGSEGRNFQFSHHLVCLDLPDHPDILEQRIG
                     RLDRIGQQQDVNIHIPYAEHSLSAVQFNWYNQVLDCIAEQNPAAGVVHDEFWPHIVLN
                     GDNTNLFAKAQDKVRQLKQAISEGRDALLEMNSCREPRASQLVTGITEFEAKTPFELV
                     EMASSLFNFHFEETQNGAYSLIPADNMLIGSLPGIPLEGVEVTFSRELANNREDVMFI
                     TWDSPFISGLWEILHHSELGCASVALLPSKQLPPGKCLLEACFDILLQSAHGKECLPF
                     LSAFSVRALVLDISDKNLSTLLPEEALQQSLKSVDKKLARKIIQSKKDEIPSWYKRAE
                     AFAEASKQQIIDHACAEVNHFYQREIHRLQDLAKRGANINDAELQALKHRHAGILTAL
                     IEQSHLQLSAIRLIVTTEV"
     assembly_gap    11094..11103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11158..12765
                     /locus_tag="PKZ52_01580"
     CDS             11158..12765
                     /locus_tag="PKZ52_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HNG58483.1"
                     /translation="MEQHKLSMHKFPRRLISLLLAASLAGCTGAGTGVTFNTSLARTN
                     VNENTQSVFWTAKAQAGANANQIKYSISGPDAAAFNFNETTGALSFKVAPDYEKPADA
                     DGNNEYIINITAQVGQSTADQNLRVTVNDITQASLALVSPKANANVGQGVPIDVETVV
                     KVDDKESNGPLNSGKVTVNGVQLTQDSSDPTLWKGFVPVLSGVVQIQVEAVTADNLVL
                     KESTQWTNSPGALKPEFMALVPGGYLATIDTTNDFIAKIFLSNIPQWVEYAGNADLFS
                     AFTMFDFNSRYQTAYFAGKDQQLLAASLASAIPKMYFGGAVAGLVGLTYDSVGNRLLL
                     MTQTAQNGSQQFAILSIGLNATQGFANAKTSETRSTAARTTPVWSVPANTIQGKFKYL
                     SYHRASKTYIISDERTVNGTAQTTVQGFSDSGQKRFEARVGADSSVLAVDEAAGVVYL
                     AEKSGTPEARLKAISLANGDVSDLLASHGEHVIGGYTDLRMDNVNKKLYVGDAISDVI
                     YVVDLASKTIAELNHIPAFTGDPTLEN"
     gene            13053..13265
                     /locus_tag="PKZ52_01585"
     CDS             13053..13265
                     /locus_tag="PKZ52_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58484.1"
                     /translation="MLRRLKLEHTSLFNSVAVTQATLIGLATATTTGAAIILGEKASR
                     YKNERGENAGTYDNFLPHIHTFTVYH"
     gene            complement(13471..>15027)
                     /locus_tag="PKZ52_01590"
     CDS             complement(13471..>15027)
                     /locus_tag="PKZ52_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNG58485.1"
                     /translation="VVDATNLQLGLSLVFELIALKRPMILVLNQMDAARARGMVIDTQ
                     GLAQDLGLPVITSIAVKRDGVDELLAALKPDQAVGYPAPHPVAESSIESIHSFIQTLL
                     GKHIHQPASTPAWQNRLDTYVMHPIWGLAILLGILLVVFQAVFAWAEPFKEAIEWGVD
                     NLKTLAQEQLPTGVLRDFLSEGIIAGVGGVLVFLPQIIILFFFILVLEDSGYLARAAF
                     LLDKPMRGIGLSGRAFIPLLSSFACAVPGIMATRTIGDPKERWISIMVAPLMTCSARL
                     PVYTLIIAAFIPTQTVLGIFNLQGLTLFALYLAGVISGALIAWLLRKRKQGHSFPLLL
                     ELPNYRWPMPYHLWMGLRERAYIFLRRVGTIILALTVIVWFLSTYPAAPAEASGAAIE
                     YSFAGVIGQFIQPLFAPLGFNWQMCIALIPAMAAREIAVATLATVYAVSIETDVSGLG
                     ANLAASWSLPVALAYLAWFVYAPQCVSTIAVVRRETNSISSTGFFVIYLFALAYGAAL
                     ITYHLAQQFF"
BASE COUNT         3731 a         3431 c         3837 g         4018 t
ORIGIN      
        1 gcgctgcatc atttccatgc caagctcttg gtgggcaagt tcgcggcctt tgaatcgcag
       61 tgaaactttg gccttatccc cttgttcgag aaagcgtatc agggccctta atttgacctg
      121 ataatccccc tcttccgtcc ctggtctaaa tttcatttct ttaatttgct gctgtttttg
      181 cttctttttc gcggcgtttt tggacttctt taactcgaac agatgcttgc cataatccat
      241 aattttgcag acgaccgggt cggcatcgac tgcgatttcc actaagtcaa gggtggcggc
      301 ctgggcggcg gcaagggctt cagcgatgct gacaatccca atttgctccc cattggcgcc
      361 gattaagcgt acttgcttag cttcaatttg ttcatttatg gccgcttttt tggagcgggg
      421 tttggcagct gctgcgggtt ctcggatgct aataatgagt tctccagtga gttagtaaaa
      481 tccaatcagc cgctaatggc tgaccctgga cttgttagcc acatcgttgg taagtaacgc
      541 gaggaagtcg tcgagggaca gggttccaag atcagtgccg gtgcgcgtgc gaacgcttac
      601 cttgcctgag gccacctcct gatcgccaag gatcagaaga taaggaacct tctgaatggt
      661 gtgctcgcgg attttaaaac cgatcttctc atttctcaag tcggcaaagg cgcgaaagcc
      721 tttttcttgc aagctttttg caacagtttg cgcaaaatcc gcctgattgt ccgtaatgtt
      781 gagcacggcc acctgctcag gagcgagcca agtggggaaa gcaccttcat agtgctcgat
      841 caaaattccg ataaagcgct caaagctacc cagaatagcc cggtgcaaca tcactggtac
      901 ttggcggctg ccatcttcgg caacaaattg agcatctaag cgagcgggca tggagaaatc
      961 cacctgaata gtgccgcatt gccacactcg acctatacag tccttgaggg agaattcaat
     1021 cttggggccg taaaaggcgc cttcgccggg cagatattca aacggcagtt gtttggcatt
     1081 gagcgcatcg gcaagggctt gttcggcctt atcccacacc tcgtcactgc ccacgcgttg
     1141 ttcgggccga gtggataaac gaataatcac atcgctaaag ccgaagtccg catatacctg
     1201 gtagagcaaa tctgtaaagg ccgacacctc tgtttggatc atattttcgc tgcagaaaat
     1261 atgggcgtca tcctgcacaa aactgcgcac ccgcatcagc ccctgcaggg tgccggaagc
     1321 ttcattgcgg tggcaggagc caaattcggc taagcgcagg ggcaagtcgc ggtagctctt
     1381 taagccctgg ttaaacacct ggatgtggca ggggcagttc atgggtttga tggcgtagtc
     1441 gcgctgttct gaggcgacgg taaacatgcc ggtttgaaat ttatcccagt ggcccgagcg
     1501 ctcccatagg ctgcgatcca ccacctgggg cgttttgatt tcttgatagc cattatcccg
     1561 ctgcaccttg cgcatgtagg tttcaatggc ggtgtaaatc gtccagcctt tggggtgcca
     1621 gaacaccatg ccgggcgctt cttcttgaat atggaagaaa tcgaactttt tgccgagctt
     1681 gcggtgatcg cgtttttccg cttcttccaa gcggtgaagg taagcgtcta aatccttctt
     1741 ggtactccaa gctgtaccgt acacgcgagt taacatggcg tttttggcat cgccgcgcca
     1801 ataggcgccg gctaccttca taagcttaaa aaacttcaat ttacctgtgg atggcacatg
     1861 ggggccgcgg cagaggtctt caaaatcccc ttggcgatag agtgaaagat cctcattggc
     1921 cggaatgctc tgaataatct cagccttgta atgctccccg agccctttga aatactcaac
     1981 cgctgcatcg cgaggcaaca cgcggcgtac aacgggaatg tcctgagcgg caagggcctg
     2041 catgcgctct tcgattttta tcaggtcatc cggggtaaag gcgcgggaat aggcaaaatc
     2101 gtagtagaag ccatcttcaa tcaccgggcc tatggtgact tgagcatctg ggtagagttg
     2161 tttgacggcc tgcgctaaca agtgggctgt ggaatggcgc agcaagtcta aggcttgatc
     2221 gctcttgtct gtgataatga ctaagcttgc gtccgactcg ataagaaacg atgcatcgac
     2281 ctcagtgcca ttgacaatgc caccgagagt cgctttggca aggcctgcac ctatattggc
     2341 tgcgacatca aaaacggaaa cagcagaagg aaattgacgg ctagagccgt cggggaggga
     2401 tacagtaatc atgggattat ctcgttcagt ggtgacccct accaagggcc acatgagtaa
     2461 aagtgccgtc ctatttcatg taaacacgtc gtttgctgca gagaccacct taactagcgg
     2521 tgagttacag ccaacaagta gttacagttt agaagctagg tggcgggtag gcggcggatt
     2581 atacaaagct tttgctgcag cgccagggtt gtggctaaat tcacgcggaa tccagcaaca
     2641 cggcagtggc cgcccgcgct ttgttaagag caagtttaaa ttctcggcag cgtttggcat
     2701 ggtcatcttg caacctacgc aacttggtat ccagtaccgc cagctgggtg cgctcataca
     2761 aggagcccga ggtttgtttt tgtaagagtt gtaactggtt caggcactca ctcacagcat
     2821 catcagtggc atcatctaag tcggccaact gattgaggag tgtttcaacg gggagcggcg
     2881 cgctcagagg cgcagggctc acttcctggg caaacttcac caacagcgtt tgtacctttt
     2941 ggctgtatgc atcgaacatg atgaccagcg cgtcgtgtag gcgcgcaaac tcgttgctgc
     3001 cagcgcgggg catattttga atcaaccaag cggcctgggc tgaccccagc ataaaccgcc
     3061 cacccaaggc ctgcatctgt gcgcctttag tttggcttaa cagctgggtt tccaaatccg
     3121 tcaggctgcc acaggtggat gctagcaact ccggtttttt gtgaatgtag agggccgctt
     3181 taagatccaa agcggtgagc gcctccaggg ttgcgtcatt gagtttggcc aaggtgctga
     3241 ccttaagggc acccaataaa taatggttga ccctgcgcag atgatcgaag ccttggttgt
     3301 cggcgttggg gttgcggata aattgctggc ggcgatagcg ttgaattaat gcgcctactt
     3361 tcgcacgcag gatttccaca tcaaaaggct ttgcaataaa gtcatcgcca ccacattgaa
     3421 acgctgtaag ttgttcatcc cgctcagtta gtccagagac aaatacaatc ggggtcaggg
     3481 cgaaatcggg gttggcgcgc atactgcgac aggtttgaaa tccatccatg cccggcatat
     3541 ctatatccag cagaattaaa tccggccgca ggctctccag catccgcagg gcctgggcgc
     3601 cgctggatgc ggtatacacc tcggcatctg ccgccagcac actgcttact agcttttgat
     3661 tgctcagatt gttgtcaacc gacaggactc gggggcgaat atccataact gtacacctca
     3721 gtactactgc ttcttttgct ttttaaatgc gagtggctaa agctatgctg ggacgggcca
     3781 ctgactgggg tattcatcca gcttcgtata gaaaaatacg cagccaagaa ccgctcaagt
     3841 caaccgttac catgccacta tgggtacaag gtaacacaaa ttactcaggc ttcggcgcct
     3901 tagttgctct aatctgggta gtagttgagg ggtttcaacg gtcgatttta aattgcccta
     3961 ttcagcgggc aaatttaacg ccgctagagc tagcagggta gtatttcaat aggctgttac
     4021 ggggcattcg catagtgaga ggtgcttatt aagcgtgatg ccaaagacta ttactaccta
     4081 ggcaccggca atgaatacac atcatttcag acacgccgtg aatacatcct aagtaggctc
     4141 gtcaaaaagg tccctctttt tgacggtctg aaatgatgtg tattcattgc ttccgtacag
     4201 ctagtaggtc aacgttttca gccaggtttc aataccattt ttttcttcaa tttgggtgaa
     4261 tctagcctgc gccgctatgg cctttgaggc actattttca gcttttattt gtgcccaaag
     4321 tgtagtttca gccgcctggc accagctgga caacatctgc ttgcctaagc cttgtccgtg
     4381 aaattccggt gctatagccc atgataacaa ttggcctgtt tggtaaggtt cggcgcggat
     4441 gtagcccaat cgttggccgg agatatcgtc tataaaaata tccaatcgtc gctgttggct
     4501 ccgcagtagg tgccgaaacc agcggcaatg ttttatatag ggtatggggg agggattgag
     4561 cgattgcatg gcgacgcttg ggtgctcgcg cacctgccaa agccagcggc aatcgctttg
     4621 gttcgcaggc cgaaaagtgg ccattagcac ttttcatttc ctaaaatttt gcgtgcagcg
     4681 ataatatttt tatcatgcgt atcatccagt tttaatggtg tggattccat aatcgcatag
     4741 cactgcttca aaaacgtcat ataagctttg cggcctattt cgtaagcgcg ctcataatca
     4801 tgttggtttt tttggaataa acaagcgcgc agcataaggg taaaatagcc gtgcatactg
     4861 ccgcgcagta acatggttgt tattgagttc ttgtcccgtt gctggcgaac gagattaaat
     4921 atttccgtaa tgcgctcgta aaaaaccttg cggttggcga tttttttagg taggcctatt
     4981 tgtttgcgca tctcgaaata attgcgcaaa taaccattga cttggctttt ttgtatgctg
     5041 ttactttctt tggggcgaat aaagtgggtt tgttttaact cttgaatgat agccgctttg
     5101 ttacctagat tggcaaaatc tttaatgtcg cttagaggtg ttggaatcgt acctttaatt
     5161 aaggcgccgt tgttggagtt atagacttta acgccgcctt tggtgcgctc gtaataagtg
     5221 agcaggattt gcatgttaac ccgcgttgaa tccagaaata cactggtaga aacctgctca
     5281 acaaaattgc ctttaataaa ataattggtt ttggtgtttt ctaacggact taagtcttta
     5341 atctgtgttt tggcggctag cgcatggtag gggcttaagg ttgagtggtg gtcgccctcc
     5401 gggctcatgc ctaaatccac cccaactaaa tatatttcag taaaacccat cgccaatgca
     5461 aatgataaac ccgcattggt aaccgttggg ttgcacaggg gtaatgcgcg gatggcggtg
     5521 ccctggcagg tttgctccac caggtcgtcg ataataatct cgccgcagtc attgggcttt
     5581 ttggcaatgc aggcgtcgga gaacattttg atcacatcgg gagcgcaggt attaaggcac
     5641 agcagggtga caccctcgcg gtcttcaggt tcggtaccca cttctaacca gtctttcaca
     5701 tcaatattgc gctccatttc gacgtggaaa tccggtttaa ttttgcgctt agataaagaa
     5761 ctgagcgcag tgccacaaga cattacaatt gcgttggagc ggttgcgctg cagaaactca
     5821 atatgtttat ccagggatgg cccgttacct accagtaaca atgggggcag gggggtggtt
     5881 ttatccgatt ggtgcagaaa aataggcgtt tggttattta ggttggcgat ggtgtgggca
     5941 aaactgactt gttcatcatc aaaaaagccg gttcctgtgg cattcaggtg aaattctttg
     6001 gtataggtgg taataaattc ttcttctttg ctactgccaa aatgccggta aataaatgta
     6061 aacaccacgt tatgcaggcc aatgcggtcg gtgaggaggc gcaattccgc catggcgtct
     6121 tgtgtttcca tgccgactaa gagtttaatc atcctatatg gacgcaggaa atattgaata
     6181 atttcacccc aatcgatggt gtggagggat gcatagaagg aatccttgtg cggatcaaaa
     6241 atgcaaagcg tgtaaatatc cagtttcgcg agtaattgag gtagctgata gcccaagccg
     6301 acacccgtca tcaaaaacat gccaataggt tgattgcagg taacttttat ggaatcgtgg
     6361 aacggtgtgt agctatctat caaatgattg accagcggtg cgtgaatgtg ggcgtcattc
     6421 attatcttgc ttttggcaaa atttaccgat gaaatcgaag gcagagcgat gaaatctgcc
     6481 acttgtttat cgataaattc gctcgggtcg cccgggtaca caggtttatt attcagcgtg
     6541 tagttaacca aatttaagtg cccatcttca gtgtacagca ggcgcaattc ttttggctcg
     6601 taatgaataa actggtggta tatttctggg gcggcttctt taaagatggc catatttttt
     6661 ttaaaccgca attcaagctg aagctgcaaa ttttgcaatt cagcctggcg taatagatcc
     6721 tggagctgat ccaaagggtt ctgtggggag gactcttgcg ctgataccat gacaattttc
     6781 tggcggtgag gaagattctg ccagtagtgc agaatctgtg ccttagccgc ttgaccctgg
     6841 ctgtgataaa aagtactgta tgcgcacaca gtaattttgg tgaggcaaca caatggcagt
     6901 aatcgctgta tggcagtgtg atcgggacgg ggctatgttt caaaataaaa aagacgccga
     6961 tgaatatgac aaaatgcttg agctggccga gtccatcggg cagtttttag agcaaaatgt
     7021 ccccggtttg agtgaggcgc agacggagtc tatcggctta ttactggccc gccgccgcga
     7081 ggcgctggtt aaggcttgta aaggtcgccc tgaggcattg tttgagccta gcgaagaggt
     7141 acaggtagag ccgtttgcga tcttacccaa gaagagctaa gtaggctttt ctagcaaaat
     7201 ccctctcctg caggggtgaa tacagatcat ggcagacgat aattagaggg atctagttca
     7261 cgagctcatc acagtgccgc acagctggcg tttgttttat tttctggcgc ctgccgcaaa
     7321 aatccgtaca atcgccgcct tttgcattag cgcgcggagc ctcttcgtgc cgggtaatca
     7381 ggtgagtcag ttatgtcgga gtttgtgcag ggtcaacgtt gggtggtgga ttcggagccg
     7441 gaattgggtc tgggcattat tatgtcggtg caagctcgta ccgtggaggt gtttttcccc
     7501 caggttgaga ctgagcgttt gtacgctaaa aaccaagcgc cacttacccg cattacttat
     7561 ggcgttgatg acaccatcaa gcttaaagat ggcagtagcg cccgcgtagt acaagtgctc
     7621 gataacaagg gctttttgct ttatgacctg ggcggtgacc gcctcgtccc cgaaactgaa
     7681 ttagccggcg atattcagct caatcaacct tatatgcgcc tgctaacagg gcagctggat
     7741 aaatccaggt ggttttattt ccgccgtcaa ttagatgacg ctttggcccg tacctggaac
     7801 tcacgcctca acggcttact gggcattcgc gccaatctta ttcctcatca gctatatgtg
     7861 gcttgggctg cctgtgagcg cgagcaggtg cgggttttgt tggctgatga agtgggcctg
     7921 ggtaaaactc tggaggcggg catgattctg acccgcctgc ttaagcttga gcgtatcggg
     7981 cgggcgctta ttgtggtgcc tgatccactg caagtgcagt ggttggtgga gctgattcgc
     8041 cgttttagct tacgcccgga gctctatgcc ggtaccgacc atgatttttc tattgggcag
     8101 attcatttaa ttccgcagtc ggtgttagcc gcccaggcgg atttattatt aaacgccgaa
     8161 ttcgattgcg tgattgttga tgaggcccac catattcaac cggccacaca gaatttttcg
     8221 gtgttggagc gtttagcgca cctatgccct catgtagtgt tattaacggc aacgccagag
     8281 caattgggct ttaacagtca ttttgctcgc ctacaactgc tagacccggc taaatacacc
     8341 gacattgagc gttttaccca agaagagaaa tcatttgctg aaattaacca gcttatccgg
     8401 cagctgccag ctagtcgcgc ccagttagtg aagcggttcg acctggataa tgaattaaat
     8461 gatgaagatt taattgcaca agtgctcgat tgccatggtc tgggcagggt gatgtttaga
     8521 aattcccgta cccaagtaaa aggttttccc gctcgaatcg cggttcccca tctattaacc
     8581 gaggatacat ggcccgcccg gtttgagtgg cttgcgcagt ttttaaaagc acacaagcag
     8641 gaaaaaatac tagttatctg ccataaacaa gcaaatgttt tagaggcgga aacatttttg
     8701 tggcaaaaac acggcttgga tgcggcctta tttcatgagg gaatgaattt ggtggagcgc
     8761 gatcgggcgg ctgcctattt cgccaatcaa gaagaggggg cacaggtttt attatgttcg
     8821 gaaatcggca gtgaaggccg taattttcag tttagccacc atttagtctg tttggattta
     8881 cccgatcacc ccgatatttt agagcagcgt atcggccgtt tggatcgcat cggccagcag
     8941 caggatgtga atattcatat tccttatgct gagcactccc taagtgcagt gcaatttaac
     9001 tggtacaacc aggtgctcga ctgtattgca gaacagaatc cagccgcagg cgtggtgcac
     9061 gatgaatttt ggccgcatat tgttttgaat ggcgataata ctaatctctt tgcaaaagcc
     9121 caggataagg tacggcaatt aaagcaggcc attagcgaag gcagggatgc gctattagaa
     9181 atgaattctt gccgagaacc gcgggctagt cagttggtaa caggtataac tgaatttgaa
     9241 gcaaaaaccc catttgaatt ggttgaaatg gccagcagcc tatttaattt ccattttgaa
     9301 gaaactcaaa atggcgccta ttcgcttatt cctgctgaca acatgctgat tggttctcta
     9361 ccgggtattc ccttagaggg agtggaggta accttttccc gtgagctggc caataaccgc
     9421 gaagatgtaa tgtttattac ctgggattca ccttttatca gcggcctttg ggaaatattg
     9481 catcactcgg agctcggctg cgcatcggtt gcgttgttac ccagtaaaca attgccgccc
     9541 ggtaaatgtc tgctggaagc ctgttttgat attttactgc aatcggccca tggcaaagaa
     9601 tgtttaccct tcctatctgc cttttcagtg cgcgccttag tattagatat tagcgataaa
     9661 aacttatcca ccctattgcc agaggaagcc ttgcagcaat cgttaaaatc cgttgacaaa
     9721 aaactcgcac gcaaaattat tcaaagcaaa aaagatgaaa tacccagctg gtataagcgc
     9781 gcggaagcct ttgcggaggc cagtaaacag cagattattg accacgcctg tgctgaggtg
     9841 aatcattttt atcagcgtga aatacaccgc ttacaggact tggctaaacg cggggctaat
     9901 attaatgatg ctgaattgca agcgctcaaa catcgccatg cgggcatact cactgcacta
     9961 attgaacaga gccatttaca gctatcagca atacgcttaa tcgtcactac agaagtttag
    10021 gtttgatttt gctgttgatt actcgtcctt gggttggcga ttattctcgc ttataaggtg
    10081 ttgtaagcgt attccgctga atcaaggcgc ttctggttga gtatttggaa cgcatagcgg
    10141 gtgagtttta tctttgcgca gcttgtatgc cgatgagcct ggcttaagat tgcccgcccc
    10201 gcttatacct ataacctaaa ccctgcctgg gtgctccatt agccttgggt ggtacatatc
    10261 cagcctgcct taattacaat tcttttgcgg ttattcatgt ttgggtcaaa ctggcgttgt
    10321 aacttgccag tctgttcaat aatcgatctg ttcggattaa atttatacga ctggcgctgc
    10381 tatgtactcg tgctgttatg aggattgagt gtgcttgcgg gtaacccgtt aagctggtct
    10441 ataggggtgt ctgccgctca tagattactt cttattgtat gagtttttat aaatagttct
    10501 tatgtgggag tatattcccc aaataaatac gattttaagg atgaataaga tctgcttatt
    10561 tttgcttgga tactttattt tccttgctac atttcgccac tttatcgaat gctttaaggc
    10621 tgaaaataaa attgtttagg agttgaccgt gcgcaaacct gagctggcga atatgtgaat
    10681 ttatcgatac aaaagaagta accactcatg tacattcggc gctctcaatt tattaaatct
    10741 aaatgcgcca attttttgca tagcccatgc tttggccatg gcgctataag gtcgagctta
    10801 gctgcttgcc actgctttat ttatataatt attaacagcc cgtgatattt cccgcgggta
    10861 ttgccctcgc tgtgccgcac ttttgcgttg caagtgtgat taatagtgat gcgttgatgt
    10921 gtgagtacca caaagtcgac ctggtcttct ctttggctaa caaatcctca aatggctgac
    10981 gattgagggt aaatatgctg cttttacaga gcggctattt gattagtatt tcaacatggt
    11041 catgagtgcg agcctgctgc aggttacggg ggcgcaggag tgtttgaaga tgcnnnnnnn
    11101 nnngagattt ttttaataca taaatagata ttacttataa caaccactgg agttcctatg
    11161 gaacagcaca agctaagcat gcacaaattt ccgcgtcgtc tgatcagcct tcttttggct
    11221 gcttcattgg cgggctgcac cggggcaggt actggcgtta catttaatac gtcactggct
    11281 cgtaccaatg taaacgaaaa tactcagtcg gtattttgga ctgcaaaagc tcaagcgggc
    11341 gccaatgcta accaaattaa atacagcatt agcgggcctg atgctgcagc gttcaacttt
    11401 aatgaaacaa caggcgcgct gtcatttaag gtcgcgccgg attatgaaaa gcccgccgat
    11461 gctgacggca acaacgagta tattattaat attaccgccc aggttgggca aagcacggca
    11521 gatcaaaatt tgcgtgtgac ggttaacgat attacgcagg cctctttagc cttggtaagc
    11581 cctaaggcta atgccaatgt ggggcagggt gtgccaattg atgttgaaac agttgttaag
    11641 gtggatgata aagaatctaa cggcccatta aatagcggca aggttactgt caatggtgtg
    11701 caattaaccc aggattccag cgatccaacc ctttggaagg gttttgtgcc cgtgctttct
    11761 ggagttgtac aaatccaggt tgaggcggtg acggccgata atttagtgct gaaggagtcc
    11821 actcagtgga cgaacagccc aggtgcctta aaacctgaat ttatggctct ggtgccgggt
    11881 ggatatttag cgactataga tactaccaat gattttatcg ccaaaatatt cttatccaac
    11941 atacctcagt gggtagagta tgctggcaat gcggatttgt tttccgcctt tacgatgttc
    12001 gattttaatt ctcgctacca aacggcttat tttgctggta aagatcagca gttattggct
    12061 gcctcgttgg ccagtgcaat tcctaaaatg tattttggtg gtgctgtagc tggcttggtt
    12121 ggtttgacct atgacagtgt tggcaaccgc ctgttgctca tgacgcaaac agcgcaaaat
    12181 ggctctcagc aatttgctat tttgtccatt ggcttaaatg ctacccaggg ttttgctaat
    12241 gctaaaacca gtgaaacccg ttcgacagca gcccgcacta ctccggtatg gagcgtgcct
    12301 gccaatacca tccagggtaa atttaaatat ttaagttacc accgtgccag caagacctat
    12361 attatttccg acgagcgcac agtaaatggt acagcacaaa cgaccgtgca aggatttagt
    12421 gatagcggcc agaagcgttt tgaagcgcga gtgggagcgg acagttctgt tctagcggtt
    12481 gatgaggctg cgggtgtggt ttatcttgcc gagaaaagcg gtactccaga agctaggtta
    12541 aaagcaatta gtttagcaaa tggcgatgtt agcgacttgt tagcttctca cggtgagcat
    12601 gttattggcg gctacacaga tttacgcatg gataatgtaa acaaaaaact ctatgtaggt
    12661 gatgctatta gtgatgttat ctatgtggtg gatctggcgt ctaagaccat agcagagctt
    12721 aaccatatcc cggcttttac gggtgatcca acactggaga actaatattt aatcttgtta
    12781 ctactagccc cagtggatac tcctctgggg cttttttatg ggtgctgttt gtaggtttaa
    12841 ctgttcggta cccttattgg cgtgttaagt gctggcggca aattgagggg aatttagcca
    12901 agtggctaga atagattctg tgggtggagt attgagagtt cgaggcataa aagcgctcgc
    12961 ttgatagttt tcagtcggtg cgataggtat ttccagcacc aataaaaaag tgctgaatag
    13021 ttacggcttt ttaagcttcg gaattttagc tgatgcttcg tcgcctgaag ctagaacata
    13081 catcgctatt taacagcgtc gccgtcacgc aggctacact gattggcctt gccacagcca
    13141 ctacaaccgg agcagccatt attcttggcg aaaaagcgtc gcgctataaa aacgagcgcg
    13201 gcgagaatgc aggcacctac gataatttct tgccacatat tcataccttt actgtttatc
    13261 attaaggcta agtataaacc tgcttaagga aataggacgc ctttaagcag ggcatgcatt
    13321 aaaattgttt atcagtactt gatagcattc agtctgcagg ggaagatatt ttgcaaactg
    13381 gccgggcagc aaatcgcacc tggaagggcg ctgcagttgc gcttgtgcga tcgcaaatat
    13441 gcccgaattc agacttgatc agagtattct ttaaaaaaac tgctgcgcta gatggtaggt
    13501 gatcagcgcg gcgccgtagg ctaaagcaaa aagataaata acaaaaaaac cggtggaact
    13561 gatggagttg gtttcgcggc gcaccacagc aatagtagag acgcactgcg gcgcgtacac
    13621 aaaccaggcc aaataagcca aggcgacggg caagctccag ctggcggcaa ggttggcacc
    13681 tagcccgctt acatcggttt ctatactcac cgcataaacc gtggctaatg tggcgacggc
    13741 aatttcccgg gctgccatgg cgggaattaa agcaatacac atttgccaat taaatcccag
    13801 tggtgcaaac agcggttgta taaattggcc tatcacacct gcgaaactat attctatggc
    13861 ggcaccgctc gcctccgctg gcgcagcagg gtaggtagat aaaaaccaca caataacagt
    13921 gagggctaaa ataatggtgc ccacgcggcg caagaaaata taagcccgct cccgcaagcc
    13981 catccacaga tgataaggca tgggccagcg gtaattgggc agctctaaca ataaagggaa
    14041 ggagtgacct tgtttgcgtt tgcgcagcag ccaagcgatc agcgcgccac ttatgacacc
    14101 agcgaggtat aacgcgaata gtgttaggcc ttgtaagtta aaaatgccca ataccgtttg
    14161 tgtaggaata aatgcggcga ttatcagtgt atacacaggc aggcgcgcag agcaggtcat
    14221 cagtggtgct accatgatgc ttatccagcg ttctttgggg tcgccaatgg ttcgcgttgc
    14281 cataatgccg ggaacggcac aggcaaagct tgataataaa ggaataaatg cgcggccgga
    14341 taagcctata ccccgcatcg gtttatccaa taaaaatgct gcccgcgcca ggtaaccgga
    14401 atcttccagc accaggatga aaaaaaataa aataataatt tgcggcagaa aaaccaatac
    14461 accacctacc cctgcgatga tgccttcact taaaaaatcc cgcagcaccc ccgtgggtag
    14521 ttgttcttga gcgagggttt taaggttatc aaccccccat tcaatggcct ctttaaaagg
    14581 ctccgcccag gcgaataccg cttggaatac caccaataaa atccccagca atatcgcaag
    14641 cccccaaata gggtgcatca cgtaggtatc gaggcgattt tgccaagctg gcgtgctggc
    14701 gggttggtgt atatgtttgc ctagcagcgt ttgaatgaaa ctgtggatgg attctatgga
    14761 ggattccgcc accggatggg gggcggggta gcccaccgct tgatcgggct tgagtgctgc
    14821 cagtaattca tccacgccgt cccgtttgac ggcgatactg gtaatcaccg gcaggcctaa
    14881 atcctgcgcc agcccttggg tgtcgatcac cataccacgg gcgcgggcgg cgtccatttg
    14941 gttgagcacc aggatcattg ggcgttttag ggcaatgagc tcaaacacta ggctaaggcc
    15001 cagctgcaga ttggtggcat ccactac
//
LOCUS       DAOQGS010000021        15007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1836_length_15007_cov_5.615235, whole genome shotgun sequence.
ACCESSION   DAOQGS010000021 DAOQGS010000000
VERSION     DAOQGS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_15007_cov_5.615235"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(565..1563)
                     /locus_tag="PKZ52_01595"
     CDS             complement(565..1563)
                     /locus_tag="PKZ52_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HNG58486.1"
                     /translation="MKLGRNDPCHCGSGKKYKHCCLDGAAKQSGEIADELAQSIAMNP
                     NLSLDELNVLLAHKMEERNNRPLNEFCGLSPHQMMNWLHAPLGELSWVTINTPADLNT
                     CPVMRYLDLLVQEAIAQGGSFKATTRGNLPAKLVKQASAVLPEFAIAQYEREISISEY
                     AGNKEDNFAALHYTRVLADIAGIFTLKNGRFWLSQSAQQQYLSGGINSLFLPMLEVAV
                     SRFNWGYLDSWESQINLRTFWLFMLWRVYSHGSLQKMFKEVATAFPDLLAHLAAEGEE
                     SPERILPIMIHLRFVKRFLQYWGFVVHNPYLWFQATEATEQSQVLPLLKQTFVFAV"
     gene            complement(1594..2385)
                     /gene="eutC"
                     /locus_tag="PKZ52_01600"
     CDS             complement(1594..2385)
                     /gene="eutC"
                     /locus_tag="PKZ52_01600"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HNG58487.1"
                     /translation="MSDDLTTPDPWQTLKTLTSARIALGRVGTSLPTQEVLNFSVSHA
                     LARDAVHLPLDVSALDAAIRACGFATLQVHSNAADRHQYLLRPDLGRRLNQSSRAQLI
                     ASATDPVDFLLVVGDGLSALAVQTQVPPLLAEIRKLLPPHWNLGRVVIASQARVAIGD
                     EIGELLKARLVAVLIGERPGLSSPDSLGIYLTFNPKIGCVDAERNCISNVRPAGLSYA
                     KAAHTLIWLAKQAIHLQLTGVGLKDESDLEWPGLSQNPVLPSPGH"
     gene            complement(2405..3802)
                     /locus_tag="PKZ52_01605"
     CDS             complement(2405..3802)
                     /locus_tag="PKZ52_01605"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNG58488.1"
                     /translation="MTYQQQVGVTNYRFADLKTLMAKATPARSGDYLAGVAASTYAER
                     MAARLCLAAVPLKQFVQEALIPYEQDEVTRLIMDSHDAKAFAPISHLTVGDFRDWLLG
                     DKADTTTLAAIAPGITPEMAAAVSKLMRNQDLILVAQKCRVVTRFRNTIGLSGHLSSR
                     LQPNHPTDDARGIAASMLDGLLYGSGDAVIGINPASDSLPALKSLYHLVDDVITQYQI
                     PTQSCILTHVTNQIQLIEQGAPVDLVFQSIAGSEAANASFGINLSLLAEAQAAALSLQ
                     RGNLGNNLMYFETGQGSALSANAHHNLDQQTCEARAYAVARHFSPLLVNTVVGFIGPE
                     YLFDGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQDDMDTLMTLLASAGLTFL
                     MGIPGADDIMLNYQTTSFHDTLYLRKLLNLRPAPEFEQWLMAMQIMDNQGRVQPISPN
                     QALLQQPPRQLRSLA"
     gene            complement(4159..5523)
                     /gene="eat"
                     /locus_tag="PKZ52_01610"
     CDS             complement(4159..5523)
                     /gene="eat"
                     /locus_tag="PKZ52_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HNG58489.1"
                     /translation="MSSSLKSSLNAWHLWGLAVGLVISGEYFGWSYGWDKAGTLGFLV
                     TTILIALMYTTFIFSFTELTTAIPHAGGPFAYARRAFGPKMAFVAGFATLVEFVFAPP
                     AIAMAIGAYLNVQFPELNPKVFAIGAYLIFVALNIIGVRIAATFELVVTVLAIVELLV
                     FMGVVSPGFSFSNFVLNGWAGASEFSSAAIPGIVAAIPFAIWFFLAIEGVAMAAEEAK
                     EPAKTIPRAYIAGILTLVFLALGVMIFAGGVGDWKALSNINDPLPQAMKTVVGENSGW
                     LHMLVWIGLFGLVASFHGIILGYSRQIFALSRAGFMPAYLGAVNSRFQTPHRAIIAGA
                     LVGIAAILVDDMQFNGMTLTANLITMAVFGAIVMYIVSMLSLFALRKKEPFLTRPFAA
                     IAYPYFPAVALVLACFSLLTMIYYNLSLALFFFGLMAVAYVYFSLTQNLRDKSVDSLV
                     HSAT"
     gene            complement(5892..7613)
                     /locus_tag="PKZ52_01615"
     CDS             complement(5892..7613)
                     /locus_tag="PKZ52_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58490.1"
                     /translation="MEKYLQIYYRAFLVLLLALNLTGCPSATPEPEYPSRPPEALADG
                     NVAIELPDYFIAGQPYSIKLKLSGRSSYTIRGNLYVTDPQFNQNGSELGDLVNNYPIT
                     LDTSNPEAIISFVPRLNGPTWDSYGVTATFKGNDSDNYTLTVKSKQIPKYERILSAGS
                     AETIVAKTEKRLSAKTNYLLEQQIGEISNIAWKQIEGPTVNLSQLSIREVSFIAPDVT
                     TTTKLRFEITAPKGHSGQNWTAEKVIYVVPTNEWVKVVKISSDGTIAVREDNSAVVAY
                     GGSEYLNFILPRPYGDIKNIVNVYYEQWFLNNRAVVILYKDGTIDAIAFDDMKRPQFL
                     FEDRERKPISGIDSYSLNEALIFRANDIKDVGVAPYDSFSFQTWDDKVFSFVGNDGFS
                     EIRNTVTGYTLNDEGVLSGDILVNSYGRAPFSLKNIASFSPQNYSDFRPEGIGYISMD
                     NQVGFLSALPSEAVDVGPKIQNEVFTPSPFQKTPFVQIAVKGRYYESFLLEKNGELSS
                     YYKGRWQAYPYYSDIAELGNVALTKDGRVVLFEGTYQAAYVGSYLAKVSTGELPSPYN
                     IKAIISE"
     gene            8025..9434
                     /locus_tag="PKZ52_01620"
     CDS             8025..9434
                     /locus_tag="PKZ52_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNG58491.1"
                     /translation="MHDIICPHCNKAFKIDETGYADILKQVRDKDFEHQLHERLELAE
                     QEKRNAVELTKAQVTRELEKAAAAQEAQIQALKATIDAGELSQQLAVRDALSAVEKDR
                     DALAHELTALKQQMQAAAQLAQANILNEAQKVAASKDAEIQALQAKLAAADLTQKLAL
                     TEAVNAAEVERTTLKNTVQRLELEKNIAEQALKEKYEMQLKDRDAHIERLKDLKAKLS
                     TKMLGETLEQHCETEFNRIRATAFPRAYFEKDNDARSGSKGDYIFRDADEAGTEIVSI
                     MFEMKNENDETATKKRNEDFLKELDKDRNEKGCEYAVLVSLLEPESELYNSGIIDVSH
                     RYPKMYVIRPQFFIPIITLLRNAAGNALKFKTELAQVKAQNIDIAHFESALDEFKTGF
                     ARNYELASKKFKTAIDEIDKTIDHLQKTKDALLGSENNLRLANNKADDLTVKKLTKGN
                     PTMAAKFAELKHDTGPAND"
     gene            9634..10617
                     /locus_tag="PKZ52_01625"
     CDS             9634..10617
                     /locus_tag="PKZ52_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG58492.1"
                     /translation="MCSWQSVLTALFFTALTSISVCAADKPAAKTPAPTDPAHFIAPK
                     MQVFNLYGEGAIPNSKPVSNEETGADKVWIQKVSQPSIQVYLPAKGKATGASVVIFPG
                     GAYWGLTFDYEGVQQANYFVDHGIAAFVVKYRLPSDTWMIDKTIGPLQDAQQAMLFAR
                     QNARQWNLDADRIGAVGFSAGGHLAASLATHFNPPLINNPANINLRPDFLVLVYPVIS
                     MDAKVTHLDSRKALLGEKPTKARIEFFSAELQVNSQTPPTLLLHAVDDTLVDIKNSLL
                     FFEALKQKGVPVEAHFLERGEHGFIRIPRDRWQGLILQWLQQKGWLGAVAR"
     gene            complement(10794..12248)
                     /locus_tag="PKZ52_01630"
     CDS             complement(10794..12248)
                     /locus_tag="PKZ52_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNG58493.1"
                     /translation="MKRRSVLIAGASCGLSACINSDGRRIKPQDDSAIAWRKGFSGVS
                     QAFKPVPLILKGHWPQDAYGNFYRNGPARTERAGIVLRHWFEGDGMVHRFNIDKTGVT
                     HQSQFVATEKFLKEEAAGRFIYPSAATIIPGAYPPRNNDSLNSANTALLPWQGKLLAL
                     WEAGSAYEIDSVSLATLGVKTWRQDCAHVPFSAHPLKERDGSLWNFGYLPYLDKGKLA
                     LYRLDYRNLMVQMGVIDLQQNGYIHACAHTQDYLIFYLPPCLYEEGQTYLDSFKWRPI
                     MGSKILLVNKRDLTPRWLDAPPGFVFHFANAWQTGSSIHVALALYQNADLMLNAMAQL
                     RAGPKPSIDHAVLTYLKISLNTGSVALDSTEINLEFPSVDLTAEGATTIYGTHAASAR
                     QHFLHDSLVAVHLEQGLISRFCFGADTIVEEPVVLKTTQNTYLMATYFNMARQYSALT
                     LFNAKRLADGPIAVAQLPYVIPLGFHGCFIPASA"
     gene            complement(12255..12683)
                     /locus_tag="PKZ52_01635"
     CDS             complement(12255..12683)
                     /locus_tag="PKZ52_01635"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HNG58494.1"
                     /translation="MRYLGFSNLLISLGFSSLMAVNTKAFELNLIIDHISKSSGNLMI
                     ALSNSEASYNDKTAAFRALKIPANQPSQTIHFGDVPPGDYAIKLYQDENDNGQLDTNM
                     LGIPKEGYGFSNNGGAMGQPAFKDAKFPVQENTTITIHLR"
     gene            13085..14203
                     /locus_tag="PKZ52_01640"
     CDS             13085..14203
                     /locus_tag="PKZ52_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNG58495.1"
                     /translation="MVYPRKWLTLAGIFTWLLIAYVDIPRSRELIELLIISAGYLSFL
                     ACFILVVTGLSQKIAPLLTHGLLFGELAIALLLIFLGSGSLTPVLLVVWAGQLPDFIS
                     RSWAFVLVLISSLGLYALLAATQPNIDPLMTGLIFFGFQLFALLSSLSRVGEQHARQH
                     AEQLNQQLLATRVLLAQSSKRDERMRIARDLHDILGHQLTALNVQLELLSHQVPQALR
                     PSVVQSKGLAQELLDTIRAVVRDQRTGFTLDIGKAIETALINLPEVRLTVQGALQLDS
                     VVLAEQLVLCLQEGISNAVRHGRATQLELLLNQQDELIVIRLVDNGRGFTGKIIPGAG
                     LTGMSERLSVYQGQVKLIPQQQGCCLTLSCSNTGKNDD"
     gene            14196..14816
                     /locus_tag="PKZ52_01645"
     CDS             14196..14816
                     /locus_tag="PKZ52_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG58496.1"
                     /translation="MISVGLVDDHQLVRAGIAGLLALNNKTQVQWQADNGARALELVE
                     QQPVDIILADIRMPVMDGISLVKALRHKAILTPVLMLTTFDDSELFAQSLAAGANGFL
                     LKDVSLEKLIHSIEALVAGEFVSNAAMLNQWRQQQTNTSARICLTPREQHILQLLVGG
                     FSNKEIGRAVFLAEGTVRNHVSHILAKLDCRDRTQAVVKALEMGLV"
BASE COUNT         3989 a         3609 c         3604 g         3805 t
ORIGIN      
        1 aaggtcattt tgccatctgg cgcgggggtt ttacacaatt ctacgaagag ccaaattttt
       61 taagtattta tactactcag aataccggtt ctgaccatgt aatattcggc cccaaagact
      121 atgttttaga taacggtagt tcagcgaaaa aggccacata gggttggtaa tattccgcct
      181 taacggtgag ctttatgtag gtggagacgg cggcaatttt aaggctagcc aatttgatgc
      241 cagcggcatt gccggaacct atgagttaga tgtaaagaat agaaaagcct aagaagcctc
      301 aaaactaaag gttagtttta atttcccgtg caatggggat acctgcaaga aataatgcaa
      361 ctgctttttt gacaaggtaa cccgtaccaa gcgaatctat gtgcagattc tatcgctccc
      421 aaaccaccgc aagcggctaa tcatgcccgt tgcagttggt atgggagcga tggtatcgac
      481 cttgcagctt ggctaataac ttggcgataa acactgacta tagaaatact cataaaacgc
      541 gactaagctc aacgtcccca ccagttaaac agcaaaaaca aacgtttgtt ttagcaacgg
      601 caaaacttgg gattgttccg ttgcttctgt cgcctgaaac cagaggtaag gattgtgcac
      661 aacaaaaccc cagtactgta aaaagcgttt tacaaaccgc aggtgaatca taataggcag
      721 tatacgctct gggctttctt ctccttcggc cgccaaatgc gccaacaaat cagggaaggc
      781 agtggccacc tctttaaaca ttttttgcaa actgccatgg ctgtaaacac gccacagcat
      841 aaataaccaa aaggtccgca aatttatttg gctttcccag gagtcaaggt agccccaatt
      901 aaatcggctt acggcaactt cgagcatggg gagaaataag ctattaattc caccgctgag
      961 gtattgctgc tgggcggatt gcgataacca aaagcggcca tttttaagcg taaaaatccc
     1021 cgcaatatcc gctaatacac gggtgtaatg tagcgcggca aaattatctt ctttattacc
     1081 cgcatattca ctaatagaaa tttcccgctc atattgcgca atggcaaact ccggcaacac
     1141 agcactggcc tgcttcacca atttagcggg caaattgccg cgagtggtcg ctttgaatga
     1201 accgccctgg gctatcgctt cctgaactaa taaatctaaa tagcgcataa ccgggcaggt
     1261 attaagatcc gctggtgtat taatggtgac ccaggataat tcacccaatg gggcatgcag
     1321 ccagttcatc atttgatggg gcgaaaggcc gcagaattca tttagcgggc ggttattacg
     1381 ctcctccatt ttatgtgcta acaatacgtt gagctcatcc aaactcagat ttggattcat
     1441 ggcgatggat tgcgctaatt catctgctat ttcaccactt tgttttgctg ctccgtccag
     1501 gcaacagtgt ttatattttt tgccgctgcc acaatggcag ggatcgttgc gccccagttt
     1561 catatttgat tttttaaata aaaggtgtgt ttttcaatgc cctggcgaag gtagcactgg
     1621 gttttgcgat aagcccggcc attctaaatc actctcgtct tttaaaccta cgccggttaa
     1681 ttgcagatgt atggcctgct tggccaacca gattagggta tgggccgcct tggcgtagct
     1741 gaggcccgcc gggcgcacgt tggagataca gttgcgctcg gcatcaacgc agccgatttt
     1801 aggattaaaa gtgaggtaaa tgcccaggct atccggggag cttaggccgg gccgctcgcc
     1861 aattaatacc gccaccaggc gcgctttaag cagctcacct atttcatcgc caatggcaac
     1921 acgggcttgg ctggcaatta ccactcggcc taaattccag tggggcggga ggagcttgcg
     1981 tatttccgcc aatagtggcg gcacttgggt ttgtacggca agtgccgaaa ggccatcccc
     2041 cactaccagt aaaaaatcca ctgggtcggt agccgaggcg attaactggg cgcggctgct
     2101 ctgattaagg cggcgcccta agtctgggcg cagcaggtat tggtggcgat ccgctgcgtt
     2161 gctgtgcacc tgcaaggttg caaatccgca ggcgcgaatg gctgcatcaa gggcgcttac
     2221 gtccaagggc aggtgcacgg cgtcgcgggc caaggcgtgg ctcacactga aattgagcac
     2281 ctcttgggtg ggcaggctcg tgcccacacg gcctaaggcg atgcgggcgg aggtgagggt
     2341 tttgagggtt tgccaggggt cgggagttgt taaatcgtca ctcatgctag cggcctctta
     2401 tggttcatgc tagcgatctc aactggcgag gtggttgttg cagcagcgcc tggttagggg
     2461 aaattggctg tacccggccc tggttgtcca taatctgcat ggccattagc cactgctcaa
     2521 attccggcgc gggccgcaaa ttcagcagtt tgcgcaaata cagcgtgtcg tgaaagctag
     2581 tggtttggta gttgagcatg atgtcgtccg cccctggaat gcccatcaaa aaggtcagcc
     2641 cggcactggc caatagggtc atcagggtat ccatatcgtc ttggtccgct tcggcatggt
     2701 tggtgtagca gatatcacag cccatgggca ggccgagtaa tttgccgcag aagtgatcct
     2761 ccagccccgc gcgaataatc tgtttgccat caaacagata ttccgggccg ataaacccca
     2821 ccacggtgtt caccaataag ggtgagaagt gccgggccac cgcataggcg cgggcctcac
     2881 aggtttgctg gtccagattg tggtgggcat tagccgaaag cgccgagcct tggccggttt
     2941 caaaatacat aagattattg cccaggttgc cgcgctgaag gcttagggca gctgcttggg
     3001 cctcggccag tagcgacaaa ttaataccaa aacttgcgtt ggccgcttca ctgccggcaa
     3061 tggactgaaa caccaaatcc accggcgcgc cctgctcaat cagctgaatt tggttggtaa
     3121 cgtgggtgag aatgcaggac tgggtgggga tttgatattg ggtaattaca tcatccacca
     3181 ggtggtacag gctttttaat gcgggcaggc tgtcgctggc cgggttaatg ccaattaccg
     3241 catcgccact gccgtacagc agcccatcga gcatggaggc tgcaataccg cgggcatcgt
     3301 cggtggggtg gttcggctgc aggcggctgg ataaatggcc gctaaggccg atggtattgc
     3361 gaaagcgcgt gacgactctg catttttgcg ccaccaaaat taaatcctga ttgcgcatga
     3421 gtttgctcac tgcggcggcc atttccggcg taatgcctgg cgcaatcgcc gcaagggttg
     3481 tggtgtcggc tttatccccc aataaccaat cgcgaaaatc ccccacggtt aaatggctga
     3541 tgggcgcaaa ggctttggca tcgtggctgt ccataattag ccgggtaact tcatcctgct
     3601 cgtagggaat taatgcctcc tgcacaaatt gtttcagcgg tacagcggcc aaacacaagc
     3661 gcgcagccat gcgctcagcg taggtgctgg cggccacacc cgctaaataa tccccggagc
     3721 gtgcaggggt ggctttagcc ataagtgttt ttaaatcggc gaagcgataa ttggtaacac
     3781 ctacttgttg ctgataggtc atggtgtttg ccccgcccaa gtggcttatg cgctgcacgc
     3841 tgcaattatt aacccggcac taaatgccgc cggggtaata atcgctgttt gagacgctct
     3901 gaataattaa taggcctttt aataaagtca ctgttttaga atgccttaag gcagcttcct
     3961 tactgtcttt tcttcccatt agttaagcat cttattaacc cggcaaaata gatcgtcctg
     4021 atctattttg ccctcgctcc gataaaactg gcgaaaatgc gtggattttc ttgttttagc
     4081 ggagctcgcg gcggcttgcg cctttggcgc ggcatccttg ccgcagttat taacccggca
     4141 taaaaagccg ccgggttatt aagtcgccga gtgcactaaa ctatccactg atttatcccg
     4201 cagattttgc gtcaagctga agtacacata ggctactgcc atcaagccga agaaaaatag
     4261 cgccaagctc aggttgtaat aaatcatggt taacaaacta aaacaggcca ataccaaggc
     4321 aactgccggg aaatagggat aggcaattgc ggcgaagggg cgagttaaaa acggctcttt
     4381 tttgcgcagg gcaaataaac tcagcattga aactatatac atcacaatcg cgccaaacac
     4441 cgccatggtg attagattag cagtcaatgt cataccgtta aattgcatat cgtccactaa
     4501 aatcgccgca atccccacca gagcaccggc gataattgcc cggtgaggtg tctgaaagcg
     4561 ggaatttacc gcacctaaat aagcgggcat aaaacccgca cggctgaggg cgaaaatctg
     4621 ccgtgaatag ccgagaataa taccgtgaaa cgacgccacc aggccgaata aaccaatcca
     4681 taccaacatg tgtaaccagc cactattttc acccactacg gttttcatgg cttggggcag
     4741 cgggtcgtta atattggaaa gcgctttcca atcccccaca ccaccggcaa aaatcattac
     4801 ccctagggcc aaaaatacca gggtgaggat tccggcaata taagcgcggg gaatggtttt
     4861 ggccggttct tttgcttcct ccgccgccat agcaacgcct tcaatggcga gaaaaaacca
     4921 aatagcaaag ggtatagctg ccacaatacc cggtattgcg gcactgctaa actccgacgc
     4981 ccccgcccaa ccattaagca caaaattcga gaaggaaaac cccggcgaaa ccacgcccat
     5041 aaataccaat aattccacaa tagccagcac agttaccacc aactcaaagg tagcggctat
     5101 gcgcacccca ataatattta gcgccacaaa aattaagtaa gcaccgatgg cgaacacttt
     5161 tggattgagt tcaggaaatt gcacgttcag gtaggcgcct atggccatgg cgatagcggg
     5221 tggggcaaac acaaattcaa ccagggtggc aaaccctgcc acaaacgcca ttttggggcc
     5281 aaatgcccgc cgcgcgtacg caaatggccc accggcgtgg ggaatggcgg tggttaattc
     5341 ggtgaaactg aaaataaacg tggtgtacat cagggcaatt aaaatagtgg taaccagaaa
     5401 tcctagggtg ccagctttat cccaaccgta actccaaccg aaatattcac cggaaataac
     5461 cagcccaacc gccagccccc agagatgcca agcgtttaaa gacgacttta aggaagaact
     5521 catacgaacc cccaaaacaa tattagcgac ccgcattgag ccgcttccgg gttgttctga
     5581 gcattatttg taccagtggg attgttagcc cggagtgttg cagccggatt aataataggt
     5641 acaggatgaa agaccgacgg cgctggtcgc catcggctag acgctattac ccacatattc
     5701 gtgggggcag aggcaactag cgccgaaata tccattacgt gccgcggtga tttacccacc
     5761 taataaactt attgccggtt tatttagcaa ttgcgccttc aaaaacttat taaataagtg
     5821 ttcgattgcg cactaactaa tgcaaatccg gcaaaaagtc cttacacgcc aattcattag
     5881 gtggtttcag attactcgct aattatcgct ttaatattgt aggggctagg taattcaccg
     5941 gtgctgacct ttgccagata gcttcctaca taagcagcct ggtaagtgcc ctcaaacaga
     6001 acaaccctac catcttttgt taatgccaca ttacccagtt cagcgatatc actataatac
     6061 ggataggcct gccatctgcc cttgtaataa gaacttaatt cgccattctt ctcaagcaaa
     6121 aagctttcat agtaccgccc cttaacagca atctgaacga atggagtctt ttgaaacggt
     6181 gaaggagtaa agacctcatt ttgaattttc ggacctacat ccacggcttc actgggaaga
     6241 gcacttaaaa agcccacttg gttatccatc gaaatataac ctattccctc tggtctaaag
     6301 tccgaatagt tttgagggga gaaacttgca atatttttta atgagaaagg cgctcttcca
     6361 tagctgttaa cgagtatatc cccgcttaac actccttcat cgtttaatgt ataacctgta
     6421 acagtgtttc taatttcact aaacccatca ttcccaacaa aactaaatac tttgtcatcc
     6481 catgtttgaa aagaaaatga gtcatatgga gccacgccta catcttttat atcattagcc
     6541 ctaaaaatta aagcttcatt taatgaataa gaatcaatac ccgaaatagg tttgcgctca
     6601 cggtcttcaa ataggaattg cggccgcttc atatcatcaa aagctatggc atctattgtt
     6661 ccatctttat aaagaattac gacggcacgg ttgtttaaaa accactgctc ataatacaca
     6721 ttaacgatat tttttatatc accatacgga cgcggtaata tgaaattcaa atattcgctg
     6781 ccaccgtacg caacaactgc tgaattatcc tctctaactg caatagtacc atcggaagag
     6841 attttgacta ctttcaccca ttcattggtc ggtactacat agattacttt ttctgcagtc
     6901 cagttctggc cactgtgacc tttaggggcc gttatttcaa agcgtaactt tgttgtggtt
     6961 gttacgtcag gtgcaataaa actaacctct cgtatcgata attgactaag gttaactgtc
     7021 ggcccctcga tttgcttcca ggcaatattg gaaatctctc ctatctgttg ttccagtaaa
     7081 tagttggtct tggcgctaag acgtttttca gtttttgcga ctattgtttc tgcactgcct
     7141 gcactaagaa tccgttcata tttgggtatc tgcttgcttt ttacagtgag tgtataattg
     7201 tcagaatcat tgcctttgaa tgtcgctgtg acgccataag aatcccaggt cggaccattt
     7261 aagcgtggaa caaaactaat tattgcctcc ggattgcttg tatcgagtgt tataggatag
     7321 ttattgacta gatcaccaag ctcactaccg ttctgattaa actgtggatc agtaacatac
     7381 aaattgcctc ttattgtata gctgctacga cccgacaatt tgagtttaat agaatatggt
     7441 tggccagcaa taaaataatc gggtagttct atcgcaacat tgccgtcagc caaagcttca
     7501 ggcgggcgac ttggatactc aggttccgga gtggccgatg gacagcccgt tagatttaaa
     7561 gccagtaata gaactaaaaa agcgcggtaa tagatctgta agtatttttc cacgtcagat
     7621 tactcctgta tgatgatgat attaaaaacg tttggcaaag gaactacccc cagaattatc
     7681 tggacgtcat aaagagtggg taaagcctac agcagactaa attaaaaaga ttggcgctgt
     7741 ctgtacgtaa aattacgtag tggcggccaa taggaaggtt gagccgaaaa atttgggata
     7801 ttgttcacgc ttttttgcgc ttgcgattct gcctgctcct cccccctatg gactttttat
     7861 ttttgaatat ttgtttctgg cccttaagat tcatagccaa ttcattcagg ctattccggc
     7921 gccaaggtta tgcgcaggca tagtcagcat cgcacctcac cgcacttatc tactaggcgc
     7981 atctcgcgta caatcgccga tataagccga aaacgagcca acccatgcac gacatcatct
     8041 gcccccactg caataaggca ttcaaaatag acgaaaccgg ttatgccgat attttaaaac
     8101 aggtgcgcga taaagatttt gagcatcaat tacatgagcg attggaattg gccgagcagg
     8161 agaaacgcaa cgccgttgaa cttaccaaag cccaggtaac gcgggagctg gaaaaggctg
     8221 ctgccgccca ggaagcgcag attcaagcct taaaagcaac aatagatgcc ggtgagctta
     8281 gccagcaatt ggcggttaga gacgcattaa gtgctgtgga aaaagaccgg gatgccttgg
     8341 cccacgaact gacagcctta aagcagcaga tgcaggcagc ggcgcaatta gcccaggcca
     8401 atattttaaa tgaggcgcaa aaagtggccg ccagtaaaga cgcagaaatt caagccctgc
     8461 aggcaaaact tgccgctgcg gatttaaccc agaaactggc cttgactgaa gcggtaaatg
     8521 ccgctgaagt agagcgcact acgcttaaaa atactgtaca gcggctggag ttagaaaaga
     8581 atattgccga gcaggcctta aaagaaaaat acgaaatgca actaaaagac cgcgatgccc
     8641 atattgagcg gctaaaagat ttgaaagcca aactctccac caaaatgctg ggggaaaccc
     8701 tagagcaaca ctgcgaaaca gaatttaacc gcatccgcgc caccgccttt ccccgcgctt
     8761 attttgaaaa agataatgac gcgcgcagcg gcagcaaggg ggattatatt ttccgggacg
     8821 cagacgaagc tggcaccgag atagtttcta ttatgtttga gatgaaaaac gaaaacgatg
     8881 agacagcaac caaaaagcgc aatgaggatt ttttaaaaga gttggataaa gatcgcaatg
     8941 aaaaaggctg tgagtacgca gtgttggttt ctctcttgga gccagaaagt gaactctata
     9001 attccggcat tattgatgta tcccaccgct acccgaagat gtatgtgatc cggccgcaat
     9061 tttttatccc tattattacc ctgctgcgca atgccgcggg caacgcgcta aaatttaaaa
     9121 cggagctggc ccaggttaag gcgcagaata tagatattgc ccattttgag agcgcgttgg
     9181 atgagtttaa aaccggcttt gcgcgcaatt atgagctggc ctcaaaaaaa tttaaaaccg
     9241 ccattgatga aatcgacaaa accatcgacc atctgcaaaa aaccaaagac gccctgctgg
     9301 gctctgaaaa taacctgcgc cttgccaaca ataaagctga tgatttaacc gtgaaaaaac
     9361 tcaccaaagg caacccaacc atggcggcta aatttgccga gttaaaacat gatacaggcc
     9421 ccgccaatga ttaaatgctg cacagcaagt acgctattct aaggaacctc tgaaaaatta
     9481 ccttactcgc cctggcgtcg ttgaatttgc caaaaaaatg ctcatttact gcgtgtaaac
     9541 tccgcttttt ttggcaaatt cgcctagcca gcgcttcgcc gcctacattt ttcagaggtt
     9601 ccctaactag attaacaata agtagggttt attatgtgtt cttggcaatc tgtattaaca
     9661 gctcttttct tcaccgcact gacctctatc agcgtttgcg ctgcggataa acccgccgcc
     9721 aaaaccccgg cccctactga ccctgcacat tttattgcac caaagatgca ggtttttaat
     9781 ctgtacggtg aaggggccat cccaaattca aagccggtgt ccaatgaaga gaccggcgcg
     9841 gataaggtgt ggatacaaaa agtttcccag ccgagtattc aggtgtattt accggccaag
     9901 gggaaggcca ccggcgccag tgtggtgata tttcccggtg gggcctattg gggtttgacg
     9961 tttgattatg aaggtgtgca gcaggcgaat tattttgtcg atcacggtat tgctgcattt
    10021 gtagtgaaat accgtttgcc cagtgataca tggatgattg ataaaaccat cggccctttg
    10081 caggatgccc agcaggcgat gttatttgcc cgtcaaaatg cccgccagtg gaatttagat
    10141 gccgaccgaa ttggcgccgt agggttttcc gcaggtgggc atttagcggc gagccttgcc
    10201 acccatttta acccaccact gataaataac ccggctaata ttaatctgcg gccggatttt
    10261 ttagtgttgg tgtatccggt gattagtatg gatgccaagg tcacccattt ggattcgcgc
    10321 aaagccctgc tgggggaaaa acctaccaaa gcgcgcattg agtttttctc cgcagaatta
    10381 caggtgaact cccaaacccc gcccaccctt ttgttacacg ccgtggatga cacattggta
    10441 gatataaaaa attccctgtt attttttgaa gcgctcaaac aaaagggcgt gccggtggag
    10501 gcgcactttt tagagcgggg cgaacacggc tttattcgca tcccgcggga tcgctggcag
    10561 gggcttattt tgcagtggtt gcagcagaag ggttggctgg gtgcagttgc tcgctaaacc
    10621 ctgtgttatg gagtctctga acattacctg actcacccag gctaaattca acgacactag
    10681 ggcgagtgag gtaatttttc agaggttcct gaatacactt ttgcccagcc tagagaaata
    10741 cctaacagcg tggtatttca gcaggcagtt aagcctttaa tgtttttagg attctatgct
    10801 gatgcaggaa taaaacagcc gtgaaacccc aagggaatca catagggtaa ttgcgcaaca
    10861 gcaatgggcc catcggctaa gcgcttagca ttaaatagtg tcagggcgga atattgccgg
    10921 gccatattaa aataagtagc cattaaatag gtattttggg tggttttaag tacaacaggc
    10981 tcttcaacaa tggtatctgc cccaaaacaa aagcggctaa ttaatccttg ttccaggtgc
    11041 actgccacca ggctatcgtg caaaaaatgc tgccgagcgg aggcggcgtg ggtgccataa
    11101 atcgtggtgg cgccttcggc agtaagatca acgctgggaa attctaaatt aatctctgtt
    11161 gagtccagcg caactgaacc tgtatttaat gatattttca gataggttaa taccgcgtgg
    11221 tcaatgctgg gttttggccc agcccgcagc tgcgccattg cgtttagcat taaatcggca
    11281 ttttgataga gggcgagggc gacatgaatg ctggagccgg tttgccaggc attggcaaaa
    11341 tgaaaaacaa accctggcgg agcatccagc caacggggtg ttaaatcccg tttattgacc
    11401 agtaaaattt tactgcccat tattggccgc catttaaaac tatctaaata ggtttgccct
    11461 tcttcataca ggcagggcgg caggtaaaaa attaaataat cctgggtgtg ggcacacgca
    11521 tgaatataac cattttgctg caggtctatc acacccatct gcaccattaa attgcggtaa
    11581 tcgagccgat atagtgccaa tttccccttg tctaaatagg gcaaataacc aaaattccac
    11641 aggctgccat cgcgctcctt taaggggtgg gccgaaaaag gcacgtgcgc acaatcctgc
    11701 cgccaggttt taacccccag cgttgccagg cttaccgaat caatttcata agcagagccc
    11761 gcttcccaca gggctaataa ttttccctgc cagggcaata aggcggtatt ggccgaatta
    11821 aggctgtcat tattgcgcgg cggataggcg ccgggaatta tcgtcgctgc ggatgggtag
    11881 ataaatcggc cagcggcttc ttccttaagg aatttttccg tggcgacaaa ttggctttgg
    11941 tgggttacac ctgttttatc aatgttaaat cggtgcacca tgccatcgcc ttcaaaccaa
    12001 tggcgcagca caataccggc gcgctcagtg cgcgctgggc cgttgcggta aaaattgcca
    12061 taggcatctt ggggccagtg gcctttaagt atcagcggca cgggtttaaa ggcttggcta
    12121 actccgctaa aacccttgcg ccaggcaatc gccgaatcat cctgaggctt aattctgcgg
    12181 ccatcgctat taatacacgc gctaaggccg caagaggctc ccgcaattaa aacactacgg
    12241 cgtttcatag gggcttagcg caagtgaatg gtaatggtgg tattttcctg cacggggaat
    12301 tttgcatcct taaaagcggg ctggcccatg gcgccgccat tattagaaaa cccatagcct
    12361 tctttgggaa tgcctagcat attagtatcc aactggccgt tatcattttc atcctgatac
    12421 agcttaatgg catagtcacc agggggcaca tcgccgaaat gaatggtttg gctgggctgg
    12481 tttgcaggta ttttcagcgc tctaaatgca gctgttttgt cgttataact tgcctcggag
    12541 ttgctcagcg caatcatcaa attgccggat gatttgctaa tgtggtcaat tattaaattc
    12601 agttcaaagg ccttggtatt gacggccata aggcttgaga agcccaagct aattaataaa
    12661 ttgctaaaac ctaaatagcg catagtgcat ctccccaata taaatggttt tttattcttt
    12721 gcacggccac atcaaacccc tcaggccatg tgttgtctcg cccggttgtg ttcaggcttg
    12781 cgcttagaat acccagccgg gggcaaaagg tcctgtgtcc cttgtcattt tatgccgtga
    12841 caaatgtcct atagctgcgc ctgctaagat ggtttaggct gtagaattta ctcgagcagc
    12901 caagctcgat gcattcctaa cagcttgtta aagtactagc gtgcgagctt tggtggtgtt
    12961 aaatgtggcg aaaaatcctt atttactcag ggcaaaatct ggttttattt gccagagcgc
    13021 cgccgccttg atatttcaat aagcggctaa tgggtggccc caagcataac gaggcgatat
    13081 tttgatggtt tatccgcgca aatggttgac cttagccggt atttttacct ggctgctcat
    13141 tgcttatgtt gatatcccgc gctcacgtga gcttattgag ctattaatca ttagcgctgg
    13201 atatttaagt tttttagcct gttttatcct ggtggtcact ggcctcagcc agaaaattgc
    13261 gccgctactt acccacgggc ttttattcgg cgaacttgca attgcgctgc tgttgatatt
    13321 ccttggcagt ggttccctta ccccagtact tttggtggta tgggctgggc aattaccgga
    13381 ttttatcagc cgcagctggg catttgtttt ggtattaatc agcagcctag ggttatatgc
    13441 cctattggcg gcaacccagc ccaatattga tccgttgatg acggggctga tattttttgg
    13501 ttttcagtta tttgcacttt tgtcctcctt aagtcgcgtc ggcgaacaac acgcccgcca
    13561 acatgcagag cagttaaatc aacaattatt agctacgcgt gttttattgg ctcaatcgag
    13621 taaacgcgat gagcgcatgc gtattgcgcg agatttacat gatattttgg gccaccaact
    13681 caccgccctg aatgtacagc tggaattact tagccaccag gtgccacagg cactgcgccc
    13741 atcggtagtc caatcaaaag gccttgcgca agaattatta gacacaattc gtgcagtggt
    13801 gcgggatcaa cgcacaggct ttactctcga tattggcaaa gctattgaaa ctgcgcttat
    13861 taatctgccg gaagtgcgct tgacagtgca gggcgcactg caactggatt cggtggtgtt
    13921 agccgaacaa ctggtgctct gcttacaaga aggtattagc aatgcggtgc gccacgggcg
    13981 cgctacacaa cttgaattat tgctcaatca gcaggatgaa ttaattgtga taaggcttgt
    14041 ggataacggc cggggtttta ccggcaaaat tataccgggc gcaggtttaa ccggtatgag
    14101 cgagcgatta tcagtctatc agggccaggt taaattaatc ccccagcaac agggttgttg
    14161 cctcacccta agctgcagca acacaggtaa aaacgatgat tagcgttggg ctggtagatg
    14221 atcatcaatt agtgcgcgca ggtattgccg gtttattagc cttaaataat aaaacccagg
    14281 tgcaatggca ggctgacaat ggtgcccgcg cgctggaact tgttgagcaa caaccggtgg
    14341 atattatact tgcggatatt cgtatgccgg ttatggacgg cattagcctg gtaaaagccc
    14401 tgcggcacaa ggcaatatta acgccggtat taatgttaac cacgtttgac gatagcgaac
    14461 tttttgccca gagcttagcc gctggcgcca atggcttttt attaaaagat gtttccttag
    14521 aaaaactcat tcacagcatt gaggctctgg tggccgggga atttgtcagt aatgccgcca
    14581 tgcttaacca atggcgccaa cagcaaacca ataccagcgc gcgtatttgc cttacacccc
    14641 gcgagcaaca tattttgcag ttgcttgtgg gtggcttttc caacaaagaa attggccgcg
    14701 ccgtattttt agccgaaggc actgtacgca atcacgtgtc acacatttta gccaagctcg
    14761 actgccgcga ccgcacccaa gcggtggtaa aagccttaga aatgggcttg gtttgagcaa
    14821 tcggctcaat tcgcacaatc gctgcgattc ataacaacta ttaacccggc ggcaaaatag
    14881 atcgtcctga acttttttgt cttcgctgca ctaaaactgg agaaaatgcg cggatttgct
    14941 tgttttagtg cagctcgcgg cggtttgcac ctttgatgcg gcatccttgc cgcctttatt
    15001 aacccgg
//
LOCUS       DAOQGS010000022        14988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1840_length_14988_cov_6.317351, whole genome shotgun sequence.
ACCESSION   DAOQGS010000022 DAOQGS010000000
VERSION     DAOQGS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 14988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14988
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_14988_cov_6.317351"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..794)
                     /locus_tag="PKZ52_01650"
     CDS             complement(69..794)
                     /locus_tag="PKZ52_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNG58497.1"
                     /translation="MKGTVKTFLPEKNYGFIKGDDGKDYFFHGSEFIDKSQVAKICEE
                     VLVDFDQEATPKGYKAKKCSLVSPCDVLTYVTPDEVILSKTSSVQGWEIIELGSWTIN
                     GSSPDSPDSARKTIIDRAASIGANALINYEYYKTTGSQGNYKYSVHNVRGQAVTVAKK
                     NSIGKHHAEDLKGLNQRAEEMKIRLIERTKESKRFRNKAWWFLGFLSFLSLFIFPFVA
                     ILFLIIGYFIGQTNEYGRWLQRM"
     gene            complement(1027..1455)
                     /locus_tag="PKZ52_01655"
     CDS             complement(1027..1455)
                     /locus_tag="PKZ52_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58498.1"
                     /translation="MQKINYFSLSFIVCLCACATVHAGDSVPLSIDSVATKRAKNVLV
                     RVIQYNTDKSALRLESIKPIRHQLLDAVDVSAFTIEGRKYEFAKCDELSVNAVAVEAE
                     QVSAEFECFVPKAAGVIAKCTIPIKAEKFEAMSCIKSGRE"
     gene            complement(1461..2339)
                     /locus_tag="PKZ52_01660"
     CDS             complement(1461..2339)
                     /locus_tag="PKZ52_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58499.1"
                     /translation="MIKVAIHASVGSKGKNMEKDIKTVTALLNVYRRSLNQTSILMSA
                     KTSAELESAIGEFQSNYLKSKSPDNKVDAGGKTFAALKLFYQTFFKAIALTPPTYGEV
                     TWNSEGAEGGKYHSRCFTVPSTSSGLTVGRGYDMKEKSQAKIASDLTSIGLASTQVDT
                     IKKAAGLSGVAAEYCVVENDLLDFQLTPAQQLALFKISYDEQAKEVERICGLDKNVKD
                     YGKVDWVKLNSSIKDIAIDLKFRGDYTSASRKLIQKPIADNDLIEFKKQLKNSANWPN
                     VPKDRFDRRVKFLDEH"
     gene            complement(2512..14319)
                     /locus_tag="PKZ52_01665"
     CDS             complement(2512..14319)
                     /locus_tag="PKZ52_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58500.1"
                     /translation="MATNNNRLNLDFTAGGDIDLNKVAIATNGGYFTAAAKGTQKLSS
                     SPDVFAAGDVKAANASISTYGALGGGDISLTAKGKADLGEMNFSYDYLNGNNFNLIQV
                     GSLAVTAGTIVLNKDVDFNDSGGYSGQGVANVHTVNNVVTFDGATDKTTLNFTAGDAI
                     TLNASIIDKEYGDGRDALGVDLIAGTGAVTINKPIYTSGGSILVSGADIIQNALINTD
                     RANERTDLEVANGKNGNATRSEGGNVTLISDADISLNTITTDSVCTVGNCTGNLFVQS
                     RTSGKKISITQADNTFLTIHGTTDFSINGGTLNLTNGQNTFGTLGGSDEAQAGRAENK
                     TITLGKTGDIRLTGFGLLLGSADITGSARLTAAGAQQSLAQVADTTFKITGDTELTAN
                     TINLVNNGNDFATISVEAASSARIHDMNGLTVNRVNTPNFTLTTVDAALFDGSIITNG
                     QPVSITQAGAGGVELADGLIKNGTGAVKFTGTALSTGLRLLSNQDATWDLASKTLVWN
                     SKTFGFDGFSALIGGGGKDTVSLSPSMNAIDLENTSAAGFLFSNIEVFKATALNTSIK
                     GFKANSQWTIIDDKKGTIAKLGGKLTAFEGFNELISSDDASNTDLFTFNGNGAIGKIT
                     GQNADVVNLFSGAGGAGVDYDWFVTANTLNGLTQVSAQVKRSGQSTDVISSITGVGHI
                     DSSNGRDTVNASATFAGTIDLKAGVNSLTTDAASLLFSSSPLNMNGFVGVNDLNAKTQ
                     AARLKATTNNASATLNWTVNPAADALKGPLSGSITYVQSGQADQTLNFNQFAFLSGGA
                     NNDTFTLNALNAGTFTGGQINAGTPGSIATQDRLINRVEGTVWDLSATSLRGVTFSGF
                     NYLDGSGTDTLNGANTTNNWRMNNGQFTLNTATQLSGFNVLNGGSGKDIFTLNGNTTG
                     ITLRGNAGSDEFILADNSIQTGMLDGGAGMGADTERDTLTLNSGTNAWVVTAAKTGTV
                     NSNSFSGMELLAGGTGSDTLDTTIANPRFLLTGIGEGILQSKTPDSTSVVSTVTGMDT
                     VKNTGALTGLDSGTLTWGITGANKGVIKNAEQKTLINFDAVGALIGGDADDVFDFTAA
                     GASLTSGVVGGKGVNTLKAPNAINTWAMAEATGGVITAAGVASEVRFEGIQRLYGGDQ
                     ADTLDYSALPASSGDATRLTIKATDLIAIESVKGNGINVFLQAGDGSNTWTFNDANTG
                     VLNNALGFSQLAGVQGGAGQDRFVLNTSSVFSGTLDGGANTLNGQAGGEVVRDELSVD
                     AQSSEFVITAQGAGRLSFTNLTHTISSLTFTGIEALSGGDAVDRFTFSNALTGAANLD
                     MLLDGKGNQDSIELPSIQLTIALAAGVERTGNKGLVLAHFESIAADEATQSSLQAMNG
                     TALWAMDGRNSGTLTLTPDANSSSPNSSNSSATNPSSTSSSSTNASPSTSTRFSGFTH
                     LVGSGGTDTFNFNAPNANGVVQGSFTGTISGGEGDRIIARDTANEWTLMAVSDGNNTL
                     KTQSGEVYVADFKGVSILQGGSEQDRFRIRGNQNAISIYGGAGNDIFTLADGGMINDI
                     HGEEGMNTLEGRISGSMVWNFISATQGQLKEGALLLAEFDGIQNFKGRSANDTVNYFS
                     SPSGVRIKTWVYEDNQFNVVEDKITLIDMDVINAGADADTVRFKAAFDGVFNAGGGSD
                     TVELLAGGSVKRFTGGTEGADSTGDKIIGPVAGAVWQLNTDNNTLADKSGTPLVGNFQ
                     DVETLQADTTGEDTLASTLADNTWNLSSANAGVFNNAIHFNGFDYLIGSDLKDSFVFN
                     FIGTFGKSVDAGKGENSVRLNLVNRVALNWGDKAVNGVKGANTIIGSNSVESELSLLS
                     DVGLTWTIDRLDGGVISDGTSSFTFDQYKNIKGTHQTDDFRFTNAGAISASIDGGEGS
                     DSLLGLNRDNTWQLGQTFSITASGDASPYVTRATGIERLLGNSGNDTFEVNARVKASI
                     AGGEGDDRLVLASGSQLDGRFNGNGGDNTIESQLAASRWELSSVVDGVIFNGDEAALG
                     YNNANKMQALASGDNQLSFANLAADVQWVINGSTNERAAGQVSVVGQTTPASQFSGFN
                     HITGAKGADDVTLAAAIGDINLGEGNNRLRLQTGAKVNSLLAGTGRDELRSFAAANLW
                     QMLSATEGTLSTASAKVVGQFTGFDVLTGSGNDRLSGAAASNKWLLNGPGSGTLNTRL
                     SFAGFGHLQGNGGNDTFEVDAAGGITSIDGGDGDDSLSARNSANTWNFSRNDSGNLRA
                     TGEQANYLSFSGIETFTGAVAAADSISLDPQVTQVKLAAGGQAGDELIVNYRDSLSLT
                     SDGTRWLGISGVQQVTAQPLTPASSAQITSSASSASRWIINEENGGDWTVGVGTAGAQ
                     TTHFRGFDTLQGAAAADEYLLRNGRISTLIDGGTALPGDLQNDSLAGDSQNTYWTVTG
                     TGAGSLTYVAEDHPPVQHFANIETLIGGAANDVFRLAADGISINIRGGNGHESVDSIA
                     FNGALDLTLGQQDVVGVGISEIEAFRADETQPNILRSSAAGGSWTLTGANAGVLVSGG
                     ERLEFFNFQNLIGGKGNDLFTLDESARVTGLLSGGEGENSLRLSHTRDLAVALGAEGV
                     GDITGSQTIAHFEHINTLSLIPLAQNLSASLTAANQSNQWVLTDNQVTLNRFNYTGFT
                     QLIGQDGADDFLLNGKQTLSIDGKGGSDSLTLTSAQGTVVGWQLTGATGTLTAAVAGN
                     TELIAPLFSNIEQLIGGAGDDVFVLTNTGNRYSRFDGGAGNNSLSNSSANNQWQLGSL
                     PGDGSAVVDRLNNAQVVRIQTLLGSGSDSLLGSDDNTLWQVGRGGQLSLSQTNSREKR
                     SLAATGISRLQGGKGQDDFVLQETGSGSFARLDGGAVGADSVTGVNTSSVRDNLDARA
                     VAGDVQVNLNEVPANTSLAANQLVVSGLETLELNPAANNTLYGPTLANNQAISWHISQ
                     AKQGSFAPTQGAHGENTVSFSGVRNLIAGEGEDRFVLATAEGLGGDLDGGAGEDYLDY
                     SQASLAASQSSFDVVIGANTRLNNIEGVIGTNTSSMRLVVKGNGSRWVLGDLDAEGTT
                     SDGINDGSVIFDALGKTVKFIDFAALVGTPGEDYFDINGGFTGSITTGEGADSLAVRV
                     NPSRAQAITLINDASDRLSLTWEGIAPAAVKETYRQAANGGESIALESLNSDPSLQPF
                     TIDFSVLASINDRLAVDEMRVQTAFESAGRNRLSLSQNALQLSIDSASGPDTSLSDAK
                     PNEAKPNEVKPSDVKPSEAKIGTWFNVENKTNLILSGQVGDQLTLSGNSQLPGTLSLE
                     KFAISAQGEQRLGAGQIHFNSVAGIGSEQAAVQIATPLVQFNNLRGDAWVTSRGDTKL
                     ANLDGQGRLNLRVDGSIGQTQRLSYTRDLTLAASGAITLDNPDNRLSGALSLSAANTM
                     TLANGITSIRALNAEHFTYTGQDLTVTGPMVVAGTTELTSTGRIDARNPANHLARLQV
                     NSAGSAALTLGADSTLVVANVGGTLQAQADGLMVTGDITAAALELIAGQPSTGAGQLN
                     VSGPLHAPQINLTAGDIVLKTALQGSELNLNAQSVSLEKPLDLQGTFSGQVDSLQQAA
                     TAAINTLGAFTLNARGVLQLDGAITSGRTISITAGGAIQQGGTWQTEGLGTTGPTKPA
                     TADIVVTSGGTITQHTHASTESTGGITYEAAGAISLTSLVAKGAVEVNTSGGIIDANG
                     TATNISGPSVRLISGADITGLDLAVDSLVGQFAGSAKTINLRNDKTLRIDRLIAGTAA
                     ENTQTTIAVSRGDVIFNNDLSKNPDVAHLYAKPDLAREKTGVVNGNFSTGTLNILAEK
                     GRVKATGSVNSNQPDLVANNVNIFSALGVSTPGRPLVIYSAGKVLYTGTGLNWKPFAA
                     FGQRYEFSSDNALDISQLLLAAGDQLIEIEPLEDVNPAVFTDIDVYATGTTSLLLPAD
                     QRYDE"
BASE COUNT         3441 a         4156 c         3597 g         3794 t
ORIGIN      
        1 caggtgaacc ttgttcgatt tcgggtgata gaaacgggac taatcgctgg gattgtatcc
       61 gtggaccatc acattctttg aagccagcgg ccatactcat tggtttgccc aatgaaatac
      121 ccgataatta agaacagaat tgctacaaag ggaaaaataa ataagctcag aaaggagagg
      181 aatcccagaa accaccaagc cttatttcgg aaccttttgc tttcttttgt tcgctcgatc
      241 agtctaattt tcatttcctc cgcgcgctga tttagccctt tcaagtcctc tgcatggtgc
      301 tttccgattg agtttttctt tgcaacggtg acagcctgac cacgcacatt gtgcacggaa
      361 tatttgtaat ttccttgcga tccagttgtt ttgtaatact cataattaat taatgcattg
      421 gcaccgatgc tggccgccct atcgataata gttttccttg ccgaatcagg cgaatcaggg
      481 gaagaaccat taatagtcca acttccgagc tctattattt cccagccctg cacactagag
      541 gttttggaca aaataacttc atcaggggta acgtaagtca aaacatcaca aggactaacc
      601 aacgaacact tttttgcctt atagccctta ggcgttgcct cttgatcaaa atcgaccaac
      661 acctcctcac atatttttgc cacttgactc ttatcaataa attcactgcc atgaaaaaaa
      721 tagtccttgc catcatcccc tttaataaat ccataatttt tctctggcaa aaacgtcttc
      781 acagttcctt tcattgcaac ctctcattgc acaatacttg aagctttagt tgtggcacct
      841 gctggacaac tttaaattta cactatctac aataccaccc atgagcagac gaggcgcatt
      901 agcacgggca tgcatttcgt caacaccata aaacacccca attgctgagc acggggtgat
      961 atcacaccag gtgattaatc gcatttaatt gaacgcgaat acgctgaaca tctgccgaat
     1021 aaacattcac tcccgacccg atttaataca gctcatcgcc tcaaactttt ccgctttaat
     1081 tggaatcgtg catttagcga ttacgcctgc agccttggga acaaagcatt caaactcagc
     1141 gcttacctgc tcggcctcca ctgcaacagc attgactgaa agctcatcgc acttagcaaa
     1201 ttcgtattta cgaccttcga ttgtaaacgc gctcacatca acagcatcca gtaactgatg
     1261 ccgaatcggc ttgatactct ccagtcgcag tgccgactta tcggtattgt attgaatcac
     1321 acgaactaaa acgttcttag cgcgcttggt tgcgaccgaa tcaattgaca atggcacaga
     1381 gtcccctgca tgcaccgtcg cgcaggcaca caaacacacg ataaaagaga gagaaaaata
     1441 gttaattttt tgcatattaa ttagtgctcg tctaaaaatt taaccctacg atcaaaacga
     1501 tccttgggaa cattgggcca gttggcagaa tttttgagtt gctttttgaa ctcgatcagg
     1561 tcgttatcgg cgataggttt ttggatcagt ttacggctcg cagaagtata atcaccccgg
     1621 aatttcaagt cgatggcaat atctttgata ctggaattca acttgaccca atcgaccttg
     1681 ccataatcct taacattttt atccagccca cagatacgtt caacttcttt cgcttgctca
     1741 tcgtaactga tcttaaagag cgctaattgc tgagcgggcg tcaattgaaa atcgagtaaa
     1801 tcgttttcaa cgacacaata ttccgctgcc acaccgctca agccagctgc tttttttatc
     1861 gtatcgacct gcgtagaagc aagcccaatc gatgtcaaat cactggctat tttagcctgc
     1921 gatttctctt tcatatcgta gccccgccca acggttaatc cagacgaagt cgatggcacg
     1981 gtaaaacacc ggctatggta tttacctccc tctgcgcctt ccgaattcca ggtgacctcg
     2041 ccataggtgg gaggtgttag cgcaatcgct ttgaagaacg tctgataaaa aagcttcaga
     2101 gcagcgaatg ttttaccgcc tgcgtctacc ttgttatccg gcgactttga cttcagataa
     2161 ttagactgaa attcaccgat agcactttcc agttccgccg acgtcttagc actcatgaga
     2221 attgacgttt gatttaggct tcgtcgatat acattaagca gtgccgttac cgttttgatg
     2281 tccttttcca tgtttttacc cttgctgcca accgaggcat gaatagctac tttaatcata
     2341 ctaactccat ttttactgtg tttataaata taaaattcaa tcaggtctcc caaccgctga
     2401 gcgcccgatg aataaattta aaaattcacg cacggtacgc ccaatccttt tcgtaaaacg
     2461 cccgacactc aatcacttga attcacgata cgaatcgatc acccgttaga attactcgtc
     2521 atagcgctgg tccgctggca acaatagcga cgtcgtcccg gttgcgtata catcgatatc
     2581 ggtaaacacc gctggattta catcttctaa cggttcaatt tcgatcagtt gatctcctgc
     2641 ggccaacaat aactgcgata tatccaaggc gttgtcactg gaaaattcat accgttgccc
     2701 aaaggcggca aacggtttcc agtttaagcc agtgccggta tacagcactt taccggcact
     2761 gtagatcacc aatggacgcc ccggtgtact cacacctaag gctgagaata tattgacgtt
     2821 gttcgctacc agatcgggct ggttactgtt gaccgagccc gtcgctttta cccggccttt
     2881 ctcagcgagg atgttcagtg taccggtgga gaaattgccg ttcaccacac cggttttttc
     2941 tcgagccagg tccggcttag cgtacagatg ggccacgtcg ggatttttgc tgagatcgtt
     3001 attaaagatc acatcaccac ggctaaccgc gatggtcgtc tgagtatttt ccgctgccgt
     3061 accggctatc agtcggtcaa tgcgcagggt tttgtcgttg cgcaggttaa tggttttggc
     3121 gctgcccgcg aactgcccca ccaagctatc caccgcgagg tccagcccgg tgatgtcggc
     3181 gccgctgatt aggcgcacgc tggggccgga gatgttcgtc gccgtgccgt tggcgtcgat
     3241 gataccgcca gaggtattca cctccaccgc gcctttggcc actaggctgg tcaggctgat
     3301 ggctccggcg gcttcatagg taatgccacc ggtactttcg gtgctggcat gggtgtgctg
     3361 ggtgatagtg ccgccggatg tcacgactat atccgccgtg gcgggtttcg tcgggccagt
     3421 ggtacctagc ccctcggtct gccaggtgcc gccctgttga atggcacctc cggctgtgat
     3481 gctgatcgtt cggccactgg tgatagcgcc atccaactgc aataccccgc gtgcgttcag
     3541 ggtaaacgcg cccagggtat tgatggccgc tgtcgcagcc tgctgcaggc tatcgacttg
     3601 gccgctgaag gtgccctgta agtcgagggg cttttctaag ctgaccgatt gagcattgag
     3661 gttcagctcg ctgccctgca atgcagtctt taacacaata tcgccagcgg taaggttgat
     3721 ctgtggggcg tgtaatgggc cactgacgtt taattgaccc gcaccggtac tcggctgtcc
     3781 ggcaatgagt tccagtgcgg cggccgtgat atcgcccgtg accatcaagc catcagcctg
     3841 ggcctgtaag gtgccgccga cgttggcgac caccagggtg ctatccgctc cgagggtcaa
     3901 tgcggctgaa cctgcgctat tcacctgcag acgggctaag tggttggcgg ggttacgggc
     3961 gtcgatacgg ccggtactgg tgagctccgt agtacctgct accaccatag gcccggtaac
     4021 ggtgagatcc tggccggtgt acgtaaagtg ctccgcgttg agggcacgga tgctggttat
     4081 gccgttagcc aaggtcattg tattggcggc cgagagggac agcgcaccgg agagtcggtt
     4141 gtcgggattg tctagggtaa tggcgccact ggcggcgagt gttaggtcgc gggtgtagct
     4201 caagcgctgc gtctggccga tggagccatc gacgcgcaaa ttgagcctgc cctggccgtc
     4261 gaggttagcc aatttagtat cgccacggct cgttacccag gcgtccccgc gcagattatt
     4321 aaactgtaca aggggtgtag cgatctgcac agcagcttgc tcgctaccga tacccgccac
     4381 actgttaaaa tgaatctgcc ctgcccccag gcgctgttcg ccttgggcgc tgatagcaaa
     4441 tttctccaga ctcagggtgc ctggcagttg actgttgccg cttagggtga gttgatcgcc
     4501 cacctgcccg cttaaaatca ggttggtttt gttctccacg ttaaaccagg tgcctatttt
     4561 ggcttcactg ggttttacgt cgctgggttt tacttcattc ggctttgctt cattaggctt
     4621 tgcgtcacta agactggtgt ctgggccact ggcgctatcg atggacagtt gcagggcgtt
     4681 ttggctgagg cttaaacggt tgcggcctgc gctttcaaag gcggtctgca cgcgcatttc
     4741 atccaccgct aaacggtcgt tgatgctggc taaaacgcta aagtcaatcg taaagggctg
     4801 caggctcgga tccgaattga ggctttcaag cgcaatcgac tcgccgccgt tcgccgcttg
     4861 tcgatacgtc tcttttacgg cggcaggcgc aatgccctcc caggtaaggc tgagcctgtc
     4921 gctcgcgtcg ttgatgagcg tgatggcctg ggcgcggctg ggattgacac gtaccgccag
     4981 gctatctgcg ccctccccgg tggtgatgct accggtgaag ccaccgttga tgtcaaaata
     5041 atcctcgccg ggtgtgccga cgagcgccgc aaaatcaata aacttcacag tcttgccgag
     5101 tgcatcaaag atgacactgc catcgttaat gccatcgctg gtggtgccct cggcatctag
     5161 gtcgcccagt acccaccggg agccattgcc cttcaccacc aggcgcatgc tgctggtgtt
     5221 ggtgccgatc acgccttcga tgttgtttag gcgggtgttc gcgccgatca ccacatcaaa
     5281 actactttga ctggcggcca ggctggcttg gctatagtcg agataatctt cgccagcacc
     5341 accgtctagg tcacccccta aaccctccgc cgtcgcaagc acaaaacggt cttcgccctc
     5401 gcccgcgatc aggttgcgca ctccactaaa gctaaccgta ttctcgccgt gggcaccctg
     5461 cgtcggcgca aagctgccct gcttggcttg gctgatatgc cagctaatag cctggttgtt
     5521 ggcaagcgta gggccgtaca gggtgttgtt ggccgctggg ttgagttcaa gggtttcaag
     5581 gccggatacc accaactgat tggccgccaa gctagtattg gcaggcacct cgttgagatt
     5641 cacctgcaca tctcccgcca cggcacgcgc atccaggttg tcgcgcacac tgctggtgtt
     5701 cacgccggtt acactatcgg cgcccacggc accgccgtcc aggcgggcaa agctgccgct
     5761 gccagtctct tgcagcacaa aatcatcctg ccctttaccg ccctgcaagc ggcttatgcc
     5821 ggtggcggct aggctgcgtt tctcgcggct gttggtttgg ctaaggctga gctgaccgcc
     5881 ccggcccact tgccagaggg tgttgtcatc gctgccgagc aggctgtcgc tgccggagcc
     5941 cagcagggtt tggatacgca ccacttgggc gttattgagc ctgtccacta ccgcactgcc
     6001 atcgccgggc aaactgccca actgccattg gttattggca ctgctgttgg acaggctgtt
     6061 gttgcctgcg ccgccatcaa agcggctata ccggttgccg gtgttggtga gcacaaatac
     6121 atcgtcgccc gcgccgccga tcagctgctc gatgttgcta aacagcggag caatcagctc
     6181 tgtgtttccg gctacggcgg ccgtgagggt gccagtggcg ccggtcaact gccaacccac
     6241 caccgtgcct tgagcggagg ttagggtcag gctgtcgctg ccgcctttac cgtcgatgct
     6301 gagggtttgt ttgccgttga gcaggaagtc atccgcgccg tcttggccga tcaattgggt
     6361 aaagcctgtg tagttgaagc gattgagcgt cacctgattg tcggtcagca cccattggtt
     6421 ggactgattg gcggcggtga gactggcgct gaggttctgc gccaggggta tgaggcttag
     6481 ggtattgatg tgctcgaagt gggcaatcgt ttggctgccc gtaatgtcgc cgacaccctc
     6541 ggcgccgagc gccacggcga ggtcgcgggt gtggctgagg cgcaggctgt tctcgccttc
     6601 gccgccggac agcaggccag taacacgggc gctttcatcc agagtaaaca ggtcattgcc
     6661 cttgccgcca atgaggttct ggaagttgaa aaactccaag cgctcacctc cggagacaag
     6721 cacgcccgcg ttggcgcccg tgagcgtcca acttccccct gcagcagaag aacgcaggat
     6781 gtttggttgg gtttcgtctg ctctaaaggc ttcgatctca ctgatgccca cacccactac
     6841 gtcctgctga ccaagggtga gatccaacgc gccgttaaag gctattgagt ccacagattc
     6901 atggccgttg ccgccgcgaa tattaatgct aatgccatcg gctgcgaggc gaaatacatc
     6961 attggccgct ccaccgatca aggtctcgat gttggcgaag tgttgcaccg gtgggtgatc
     7021 ttccgcgaca taggtcagtg agcccgcgcc ggtgcccgtc acggtccaat aggtgttttg
     7081 gctgtcccct gccaggctgt cgttttgcag gtcgcccggc aacgcggtac cgccatcaat
     7141 tagtgtactg atccggccat tcctgagcag gtattcatct gcagcggcag ccccctgcag
     7201 cgtatcgaag cccctaaagt gcgtcgtttg agcacccgcc gtgccgaccc ccaccgtcca
     7261 atcaccgccg ttttcttcat tgatgatcca gcgactggcg gagcttgcgc tactggttat
     7321 ttgcgcacta gacgccggag taagtggctg ggcggtcacc tgctgcacac cactgatccc
     7381 caaccagcgg gtaccatcac tcgtgaggct gaggctgtcg cggtaattca cgattaactc
     7441 atctccggct tggccacccg ccgccaattt cacctgggtt acctgtgggt ctaggctgat
     7501 gctatcggcc gccgccacag cgccagtaaa ggtttcaatc ccgctaaaac ttaagtaatt
     7561 ggcctgctcg ccggtagcgc gcaggttgcc gctgtcattg cggctgaagt tccaggtatt
     7621 ggcgctgttg cgagcactta ggctgtcgtc accgtcgcca ccatcaatac tcgttatacc
     7681 gcctgcggca tccacttcga aggtgtcgtt gccgccattg ccctgcaggt ggccaaagcc
     7741 tgcgaagctg agcctggtgt tgagggtgcc gctgccgggg ccgttcagca gccatttatt
     7801 ggatgccgcc gcgccgctta agcggtcatt gcccgagccg gtcaacacgt cgaagccggt
     7861 aaactggccc accaccttag cgcttgcggt acttaatgtg ccctcggtcg cactgagcat
     7921 ttgccacaga ttcgcagcgg caaaacttct cagctcatca cggcccgtgc ctgctagcag
     7981 gctgttcacc ttggcgccgg tttgtagcct gaggcggtta ttgccctcac ctaaattgat
     8041 atcgccaatg gcggcggcga gggttacatc gtcagccccc ttggcaccgg tgatatgatt
     8101 aaagccgcta aattggctgg cgggagtggt ttggcccacc accgacacct gacctgcggc
     8161 gcgctcattc gtgctgccgt taatcaccca ctgtacgtct gctgccaggt tggcaaaact
     8221 taattggtta tccccactgg ccagtgcttg catcttgttg gcattgttgt accccagagc
     8281 ggcttcgtca ccattgaaga taacgccgtc caccacggag gataattccc agcggctggc
     8341 cgccaactgg ctctcgatgg tgttgtcacc gccgttgcca ttgaagcggc cgtccaattg
     8401 gctgccggag gcaagcacca aacgatcatc gccttcacca ccggcaatac tagctttaac
     8461 ccgagcattg acctcaaagg tatcgttgcc gctattcccc aacaaccgct cgatacccgt
     8521 agcacgggtt acataggggc tggcatctcc tgaggcggta atcgagaagg tttggccaag
     8581 ctgccatgtg ttatcgcgat taagccccaa caggctgtcg gagccttcgc cgccgtcgat
     8641 actggcgctg atagccccgg cattggtaaa ccgaaaatca tccgtctggt gtgtgccttt
     8701 aatattttta tactgatcga aggtaaagct cgaggtgcca tcggaaatga caccgccatc
     8761 taagcggtca atggtccagg tgaggccaac atcgctgagc aggcttaatt cactctccac
     8821 actgttggag ccaataattg tattggcgcc tttaacgcca ttcacagctt tatcgcccca
     8881 attaagcgca acgcggttaa ctaaattcaa ccgcacactg ttctcgcctt tgcccgcatc
     8941 cacgcttttg ccaaaggtgc caataaaatt aaaaacaaaa ctgtctttta aatccgagcc
     9001 gattaaataa tcaaagccgt taaagtgaat ggcgttattg aatacacccg cattggccga
     9061 tgataggttc caagtgttat ccgccagcgt agacgctaga gtgtcttccc cggtggtatc
     9121 ggcttgcagt gtttccacgt cttgaaaatt acccaccaag ggtgtgccgc ttttatcggc
     9181 gagggtgttg ttatccgtat ttaattgcca aacggcaccg gccacgggac cgattatttt
     9241 atcgcctgtg ctatcggcgc cctcggtgcc accggtaaaa cgctttaccg aaccaccggc
     9301 taataattcc accgtatcgc tgccgccacc ggcattaaaa acaccatcaa atgctgcttt
     9361 aaatcgcact gtatcggcat ccgcgcccgc attaatgacg tccatatcaa tcagggttat
     9421 tttatcttca accacattaa attgattatc ctcgtagacc caggttttta tacgcacccc
     9481 actgggtgaa ctaaaataat taactgtatc gttggccgag cggcctttaa aattctgaat
     9541 gccgtcaaac tcggccaaca gcaacgcgcc ctcttttaat tgaccctgtg tagccgagat
     9601 aaaattccat accatgctac cgctgatacg tccttccagc gtattcattc cctcctcacc
     9661 atggatgtcg ttaatcatgc cgccatcagc gagcgtaaaa atatcgttac ccgcaccacc
     9721 gtaaatactg atggcgtttt ggttaccccg aatcctaaag cgatcctgct cgctaccccc
     9781 ttgaagaata ctgacgccct taaaatccgc tacatacacc tcgccggatt gagttttaag
     9841 tgtattgttg ccgtcactta cagccattaa tgtccattcg ttggcggtat cccgcgcaat
     9901 aatacgatca ccctcaccgc cgctaatcgt gcccgtaaaa ctaccctgca ccacgccgtt
     9961 ggcattgggc gcattaaaat taaaggtatc cgtaccaccg ctgcccacta aatgggtaaa
    10021 accactaaaa cgtgttgagg tgctgggact ggcattggtc gaactggaac tggtggaact
    10081 gggattggtg gcactggagt tagaggaatt ggggctggag gaattagcat ccggggtgag
    10141 cgttaaggtt ccagaattgc gcccatccat cgcccacagg gctgttccat tcatggcctg
    10201 caaactgctt tgggtcgcct catccgccgc gatggattca aaatgcgcta acaccaagcc
    10261 tttattaccc gtgcgctcaa cacctgccgc aagggcaatc gtgagttgaa tgctgggcaa
    10321 ttcaatggaa tcctgattcc ctttaccgtc caaaagcata tcgagattgg ctgcaccggt
    10381 cagcgcgttg gaaaaggtaa accgatcaac cgcatcgccc ccactcaagg cttcaatgcc
    10441 ggtaaatgta agcgatgata ttgtatgtgt aagattggta aaactcagac ggcccgcgcc
    10501 ttgtgcggta attacaaatt cactggattg cgcatccaca ctcaattcat cccgcacgac
    10561 ttcgccaccg gcctggccgt tcagtgtatt agctccgccg tctaaagtgc cgctaaatac
    10621 gctgctggta ttcagcacaa aacggtcttg gcctgcaccg ccttgcacac ccgccaactg
    10681 gctaaaccct aacgcattat ttaaaacacc cgtattggcg tcattgaagg tccaggtatt
    10741 gctgccatcc cccgcctgta aaaatacgtt aatgccatta cctttcaccg attcaatcgc
    10801 gattaaatcc gtagccttta tcgtcaagcg cgtcgcatcg ccactgctgg caggcagagc
    10861 agaataatcc agggtatcgg cttgatcgcc accgtacaat ctttgaatac cctcaaaacg
    10921 cacctcagat gccacgcctg ccgctgtaat cacaccaccg gtagcctcag ccattgccca
    10981 ggtgttgatg gcattaggcg cttttaatgt attaacgcct ttgccaccga caaccccaga
    11041 cgttaaagac gcacccgctg cggtgaaatc aaacacatca tccgcatccc caccgattaa
    11101 tgcgcccaca gcgtcaaaat taattaaggt tttttgctca gcatttttaa tgacgccttt
    11161 attcgcaccc gtaatacccc aggtaagcgt gccgctatct aagcctgtta atgcaccggt
    11221 atttttaacc gtgtccatac cggtaaccgt gctaacaacg gatgttgaat cgggagtttt
    11281 tgactgcaga attccctcgc ctataccggt caataaaaag cgtggattag cgatggtggt
    11341 atccagcgtg tcactgcctg tgccaccggc taaaagctcc ataccactaa acgaattact
    11401 attcaccgtg ccggttttgg ctgccgtgac gacccaagca ttggtgcctg aattgagtgt
    11461 aagcgtgtcg cgctcagtat cggcacccat ccctgcaccg ccgtccagca ttccagtttg
    11521 aatactgtta tcggccagaa taaattcatc tgacccagcg ttaccgcgca gtgttatccc
    11581 cgttgtattg ccattaagcg taaaaatatc cttaccgctg ccgccgttca atacattaaa
    11641 gccgctcaat tgagtggccg tatttaacgt gaattgacca ttattcatac gccaattatt
    11701 ggtggtattc gctccattaa gggtatccgt gccactgcca tctaaataat taaagccact
    11761 aaatgtcacc ccccgcagtg aggtcgccga taaatcccat acagtgccct caaccctatt
    11821 aattaaccga tcctgtgtgg cgatactacc aggtgtaccg gcattaattt gcccaccggt
    11881 aaatgtgcct gcatttaatg catttaatgt aaaagtgtcg ttattcgcgc cgccggataa
    11941 aaacgcaaat tgattaaagt tcagggtttg atccgcttgc ccggattgta cataggtgat
    12001 agatccactt aacgggcctt taagggcatc cgccgctggg ttaaccgtcc agttcaaggt
    12061 agcgctggca ttattcgtag tggcctttaa acgcgcggcc tgggttttag cgttgaggtc
    12121 attaacaccc acaaacccat tcatgtttaa tgggctgctg gagaataaaa ggctcgcggc
    12181 atccgtcgtt aaactattaa caccggcttt taaatcaatg gtacctgcaa aagtcgctga
    12241 agcattaacg gtatccctgc cattactact atcgatatgg cccacgccgg taatagacga
    12301 aataacatcc gtactttggc cactgcgttt tacctgagct gatacctgcg taaggccatt
    12361 cagggtgtta gcggtaacaa accaatcata atccacccca gcgccgcctg cgcccgaaaa
    12421 cagattaacg acatcggcat tttggccggt aattttgcca atagcaccat tgccattaaa
    12481 cgtgaataga tcggtattcg aggcgtcatc cgagctgatt aattcattaa agccttcaaa
    12541 tgccgtaagt ttaccgccta attttgcaat ggtgcctttt ttatcgtcga taatcgtcca
    12601 ttgagaatta gctttaaagc ctttgatgct ggtgttcaac gcagtggctt taaacacctc
    12661 tatattcgag aataaaaaac cggcagctga tgtattttcc aaatcaatgg cattcatact
    12721 tggcgataag ctaaccgtat ctttgccgcc accaccgata agtgcgctaa aaccgtcaaa
    12781 gccaaaagtt ttactgttcc acactaatgt tttactcgct aaatcccagg tcgcatcctg
    12841 attgctcaac agccgtaaac ctgtgcttaa tgccgtgcca gtaaacttaa cggcacccgt
    12901 accatttttt attaagccat ctgccagctc aacgcctccc gcacccgctt gggtaatcga
    12961 gaccggttga ccgtttgtaa tgatagagcc atcaaacaag gccgcatcta cggtagtgag
    13021 cgtaaaattc ggtgtgttta cacggtttac tgtcaggcca ttcatgtcgt gaatacgtgc
    13081 cgaagatgcc gcctctacac tgatcgtcgc aaaatcgttg ccgttattaa caaggttaat
    13141 cgtgttggcc gttaattccg tatcgcccgt aattttgaac gtggtatcag ccacttgcgc
    13201 aagggattgc tgagcgccag ccgccgttaa acgcgcagaa ccagtaatat ctgcagaacc
    13261 cagcaacagc ccaaaccccg tcaaccggat atcgccggtt ttgcccagcg taatggtttt
    13321 attctccgca cgccccgctt gcgcctcatc gctaccgccc aaggtgccaa atgtattctg
    13381 cccattggtt aaattaagcg tgccgccatt aatactaaaa tccgtagtgc catgaatggt
    13441 taaaaaggtg ttatcggctt gagtaatact gatttttttg ccgctggtgc ggctttgcac
    13501 gaagagattt cccgtacagt tgccaaccgt acacactgaa tcggtggtaa tagtgtttaa
    13561 cgaaatatcg gcatcgctga tcaaagtaac attgccgcct tccgagcgag tggcattgcc
    13621 atttttgccg ttggccactt ctaagtcagt gcgctcatta gcgcggtcgg tgttaatcag
    13681 cgcgttttga ataatgtccg caccagacac caggatagaa ccgccagagg tataaatggg
    13741 tttattaatg gtgaccgcgc ctgtaccggc gattaagtca acccccaatg catcgcgccc
    13801 atcgccatac tctttgtcga tgatggatgc atttaaggtg attgcgtcgc cagcagtgaa
    13861 attaagggtc gttttatcgg tagcgccatc aaaggtcaca acattattta cggtgtgcac
    13921 attggcaaca ccctgccccg aataaccacc gctatcgtta aaatcaacgt ctttattcag
    13981 cacaatagtg cctgccgtta ccgccaggga acccacctgg attaaattaa agttattgcc
    14041 gttaagatag tcataactaa aattcatctc ccctaaatct gccttaccct tagcggtgag
    14101 gctaatatca ccaccgccca aagcgccata ggtactgatg ctggcattag cggctttaac
    14161 atcaccggca gcgaatacat cgggggagct ggacagtttt tgcgtgccct tggcggcagc
    14221 ggtgaaataa ccgccattgg tagcaatagc aaccttattt aaatctatat caccgcctgc
    14281 ggtgaagtct aaattgaggc ggttattatt ggttgccacc aaggtggtgg ctttgtcgga
    14341 gaattttata tcgccgtcgg cgttaagttt taacgtagag gttatgttgc caacattgtc
    14401 gaaagtaaaa ttaaaacttg gattattacc tgtgtaatca aaagtaatgt taccgacccc
    14461 agcaccagct ccagtccgaa cctcaacagt tcccccattt aataatgccg tattgatgtc
    14521 gccccatcga attacagacc tgctattgtt cgacgtatat acatttccaa ctgggcttac
    14581 attggtcgaa atatttccac taatggtcaa gttgtaggga tcaatcaacc atcccccagc
    14641 tacgccgttt attgctgata tgtcaggact gccttccaat atcaacccat tcaatcccga
    14701 cgtttctaca aatccaccat tgccgcttat cgcaccccct ttagcttcaa gctgtcccag
    14761 taaagttgcc gtatcggacg cgaaggcaat aatttttcct ccgtctcctc gctctagtgc
    14821 atttgatttc gtttgcgtgg ttgcaccaat atgtaaaaac tgagcattgc taatcagtgg
    14881 attagcccct gttttatcgc cgcccaataa aatctgccca ccgccattcg aacccgaagc
    14941 atctactcgg gcgctatcca ataggccaac tttctcaccg agcaattt
//
LOCUS       DAOQGS010000023        14779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1864_length_14779_cov_4.519764, whole genome shotgun sequence.
ACCESSION   DAOQGS010000023 DAOQGS010000000
VERSION     DAOQGS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 14779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14779
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_14779_cov_4.519764"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2545
                     /locus_tag="PKZ52_01670"
     CDS             <1..2545
                     /locus_tag="PKZ52_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58501.1"
                     /translation="PGVPANHAISRINGGANDGHIVFGFDLFSPIFTEPAISGSGTIT
                     SDYGFANNIALPVSNVTQGGDFLTLHHSGTPGSDEGRAVQFFDLLGGADGSLNQQRVA
                     DEVASHRWATGSVPIDVRPIALAINKGCLTIDAASNQVVSTTGTALGVNQAFFTARSG
                     MSINQLVTDTRTRAQSLPRRKADFQKFNLTRVGDIYLPGGGNGLIEEYGPTTPTGVST
                     SLSRIRQEIFRRPAGGSGDATIMGGKYVDREDYGYNTERVAMYRYFLEPLGVSVDKWS
                     MGVRGRSRTYTFADVFDDYLTPFTAGIEANLVAEPLAGLSVPTNCSSLIPAVNATLAA
                     LPGVNDVPTYTDVQRIFNKSCIECHGGLNYPPVSTLGVGYLDFSEDENAPFGHRLDRS
                     HGFAFGFVTNTPATSYLYQRITNTSEDCPSGMMPCGGPALPSTDQETIRRWIVGGAPL
                     TIGDPHITTVNGRNFDFQSSGEFVLLKGINEEIQVRQTPFGAEVPITDPNTGIAACPS
                     LSTAVALNVGGQRISYQPNISGEPDPKGMQLRIDGKLVSLTSSGINLANGGRIMRTPA
                     AGGVQVEFPGGSAVVITPYFWDYYQVWYLAVDVRNARSTEGVMGSVAPGNWLPALPGG
                     DLLGGLPASTSDRFDVLYSKFADAWRVDDKTSLFDYAPGTSSKTFMLPGWPKNGAKNC
                     YVPKIPGIAVREPVKPMDLKLAQQYCSTVKNPALRKACSQDVATTAEPAFAKPYLTVE
                     QLQLNNKPLPVELAYPKNNQTDVSEKTTFTWKPTLDKDGDKLTYRFCLWASGEKLTFD
                     RCTLLPEQKDLGFFVPSNLKVSQAYFWKVIAEDGKGGTTDSPTWRFAVK"
     gene            3420..6347
                     /locus_tag="PKZ52_01675"
     CDS             3420..6347
                     /locus_tag="PKZ52_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG58502.1"
                     /translation="MNTRAQLTDQPTRSPTDGRVPSFTRALLTNVVGVVIVLELVLLI
                     GMAWQAYTSTHQAARTKAQETQARVSERLRILIRAAEMTGESAERAARTPEISSATLR
                     STIEHSLAAFEQRPELSYLGIVLADNGAYGNLERTASGDILLWLFPGQDAKGEVIRSF
                     LQTRAGFKPHSQLPGYEYDPRTRPFYQAAVNSPSGETWMPTYQWIVHENTSESLWGFS
                     YVKALRSEDGRLLGVLDSDFDLPALNRFLRNLEQEYQTHIQVVELGPTHRLIGAASLK
                     QEPMPLSAELSALVKIAQADYVAKRPHGGEHHWVAANRLRLKGGLDWLVITSSPSPFI
                     EAPLLRQLYQVGAMGLLIALGLMWVSVRMARRFGAPLAELERQVNQIGLQELSLTTPL
                     SNTRAAQFRETQLLGQALDHLRLVVGQYVESKEQQAASLTLKSVIFDSSKTAILSLDD
                     KLQILEMNRAAERLFGRYREQFNGKDVRTLVHLNDPALSWELIRASKGIKTTQFKGPC
                     GVFDAEFRVANFKSGDTQVCTLFIDDISERKQAEARIRYLATHDDLTGLPNRTLIMDR
                     IVQAIRHARRAGSQLAILYLDLDRFKVLNDGYGHSFGDAVLKAAAERLGALVREGDSV
                     SRQGGDEFLILLTGLHSAADAYIATEKIVKQLDAPILVDNRKVHLSGSIGVSVFPHDG
                     DTPETLIDHADVAMYRAKDLGRNTYQFFTPEMSAETQWRVNLEHHLREAIAENQFTLA
                     FQPKVNLASGRIVGCEALLRWRHPKLGAVSPDQFIPIAEDSGLIVPIGDWVLRTACAQ
                     AKAWSDNCQKQPCIAVNISARQFLQQDVASWVIRTLEETGLPPNQLELEFTESLIAKD
                     VKKVIATFGQLRAIGVKLSIDDFGTGYSSLSYLKRFPLDTLKIDQSFVRDMLTDPEDA
                     AIVRAVISLAHSLEFNVIAEGVETEAHNAFLLQNECDEIQGYYFSKPLPAAEFEALLA
                     EAM"
     gene            6796..8976
                     /locus_tag="PKZ52_01680"
     CDS             6796..8976
                     /locus_tag="PKZ52_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG58503.1"
                     /translation="MKCPTLLPDEPERLQALARYGFEQGQMLKSLDPVVQIASHMFAM
                     PVAAVNMIGSDHVFFGAAVGIEYRDLSTLERSREVSFCAHAITQDEVMVVADASCDER
                     FFDNPLVTGNAQVRFYAGVPLRSPEGHALGALCILDTKPHPEFSTADAKRLAELAKMA
                     SDRLELRRIEVASTRFRPPFASYAANLPSAVIWFDEHLQLLEWNQPAADFLGFPLEAK
                     PGLTFDALLAPGARAGFRRLIADACAQASLEQVHFPEELTVLGAHGQEIPIGLVLLGW
                     REAGRMTFEVIFKDTSHEPTHTGLDDLTGLANRGTFYRRVENHLLQSPPDGAVLVIDL
                     DGFKDLNDTLGQGLGDDILRAVAERLSQSVDSGTLVARLGADEFAVLHPQPLTPAAAL
                     SLAQHLTLALTQPLSLRGQDVRISACCGLALAPQQAQEAIELVSNADLALFKAKTCGP
                     GRTQLFDPNLRQEAVTRRLYGLELHRAVDKGEFLLFYQPQINMADGTITGAEALIRWR
                     HPERGLLSPASFIHTLENGPLAATVGSWVLNEACAQAAHWRRMFNNDFRIGINLCGAQ
                     FYGGSLTEDVLATLARHGLPPAALELEVTENIVLANADLILDHLQQLRARGIGIAFDD
                     FGTGYASLSLLKSYPLSRIKIDQSFVRGIATSKGDASVVRAILDMARNFELETIAEGI
                     ETSEELDFLQREHCTEGQGYLFGKPMPAWQFETLLAADRQADIA"
     gene            9162..9542
                     /locus_tag="PKZ52_01685"
     CDS             9162..9542
                     /locus_tag="PKZ52_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58504.1"
                     /translation="MNLRTLNNQIRRSEAKIAIQLAKNNCHNKTQEINFEWLITWAIK
                     KANKKTPLWCGNVKIMSLYKTDSRQFYITATIHIGSELDTSNQLAEATLHGHMLLNGH
                     KNRLKSYLLTITHNDEEYKLHKPA"
     gene            10043..12625
                     /locus_tag="PKZ52_01690"
     CDS             10043..12625
                     /locus_tag="PKZ52_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58505.1"
                     /translation="MTIKNIKILLAVLLLSLEGIAELPCAEATTNVLGASPQRHDRFY
                     QGADKQFILRQYDLSGIGKVVSASSKWATMVSERFFVSSNHAHPSSGEVVRFYFSNNP
                     ADGFVDRTVAGGEQIKDSSGRAVGDLWLGYFSAPVPVNIALYPLLGSGMAYPPIDSKL
                     ILLGKANDGLQAELNPLNQRVGRNICVAHEIANHAYFYTYDPIGAADSLGVDENSVVA
                     GDSGAPTFILIDNKLVLVGTRWGFDASLKVDIDSSVIAKTTPLAQAIARLSANSEQLR
                     ILNIPARLTGAVLLEDFEAEQLGATTLIGSPTTTKHLITGTDAGGRAEVVARNNNQQL
                     KLTSPSDADTDSPKLFFETIATAISQGEISLRFRIDPISQQPPVSNKPILTLRAGDDI
                     GLNQIALDGPIAQAAIQIEFTDTGHLNITTADGSLLSEQQFTFGQEHFFAIKFNLSAK
                     NFSLYLDGDPLSANSVKQFTPISAVKKLDAIYVASRGLPNTSTTAYVDELHIDAPGGW
                     RSLEQTLALPVLGANLENDIFSSNYWQRVANTTNSILDVGAGKANAGAWRGFSRLGIN
                     GFNSSEIAQIIDLTASGLNKDIDKGGVTATYSAAGMSEQIGDYAHLGMEFLNADKVSI
                     DQAPQVAINDGKSRWAPLQLQHLVPAATRYLKLTAGVTRSAGITTDAYIDGLFSANLS
                     WVKTQAILPVDVQARDLEPAELINQYWQKLSNNTGVFIEFANVGINNTGTSGVARGGI
                     SGFNRAELAQTLDLTTSPYLIDIDAGKVFINASALGISYQVGDYAQLKLEFFDNTGIL
                     LATSPIVAVNDGQGQWAAMKITEYKVPIKTRKVRLVIGLTRSSGVTTDAYIDGPIKVF
                     FSRA"
     gene            complement(12656..13867)
                     /locus_tag="PKZ52_01695"
     CDS             complement(12656..13867)
                     /locus_tag="PKZ52_01695"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNG58506.1"
                     /translation="MAKIIGICELGGYLGHISKITSLLQRLHALGHEVVAVLPDISFV
                     GFMQAQEPPIAYVLGPKLPMPAFKTSRDPVNLSEVLLCAGLGTDSVLTPCAHVWRQLL
                     TLLKADLILADYAPTPLLAARSLGLPAVVLGTGFSVPPPVSPLPAFNRRLVIQEAALK
                     ASDEQLLAALNRYLAKFNAPLLNCVSDLFWQTAQVAITHAQELDHFAQGRPADTQYYG
                     LATQMPGGLAVPWPAGQGKRVFAYLKTEYTGLEYLLTLLAAMPVVAVVYISGAQAEHY
                     QTFATERLQIYLAPVDLTELMPQAEWLIYHAGSGMASQALVCGKPSILIPTHYEQLFT
                     AEALASRHLGYVLNPHWPGAQIEALVRQALQDIAMEQRCAALAGAGNNLGQDELVACI
                     DGVARHPCRHS"
     gene            complement(14027..14650)
                     /locus_tag="PKZ52_01700"
     CDS             complement(14027..14650)
                     /locus_tag="PKZ52_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNG58507.1"
                     /translation="MFGSFYADNFYLDTLATYGKNNYDMVRSVLDGAAVKSLANADTD
                     GSETSFGLGAGYQFDFKTMQLKLFTRLDYIDVGVDSYAEFSGDDTMLVAKINDFSIKS
                     FTSNLGVSYTWIINTSFGVLSPELGVDYEHQFKGDGFNVDGQFIGGEAGEFSISTFDK
                     DANYFNGNLALNAVFTQGVSAYLSYTGQFGRDKWSSDHYALGARMEF"
BASE COUNT         3618 a         4387 c         3677 g         3097 t
ORIGIN      
        1 gccaggcgta ccggccaacc acgctatcag ccgcatcaat ggcggcgcca acgacggcca
       61 tatagtgttc ggttttgact tgttttcgcc catatttacc gagcctgcta tcagtggcag
      121 tggcacaatc acttctgatt acggctttgc caataacatc gcgctaccag tctccaatgt
      181 gactcaaggc ggtgattttt taaccctgca tcactccggc acccccggca gcgacgaggg
      241 ccgtgctgta cagttttttg atttgctcgg cggcgccgat ggcagcctca accagcagcg
      301 agtggcagac gaagtggcca gccaccgctg ggcaaccggc agcgtgccca ttgatgtaag
      361 gcccatcgcc ctggcaatta ataagggctg cttgactatt gatgcagcca gcaaccaggt
      421 tgtcagcacc acgggcacag ccctaggtgt taaccaagcc ttttttaccg cccgcagtgg
      481 tatgagcatt aaccaattgg ttaccgatac gcgcacgcgc gcccagagct tgcctcgccg
      541 caaagcagac tttcagaaat ttaaccttac ccgcgtgggc gatatttacc tacccggcgg
      601 cggcaacggt ttaattgaag agtacggccc caccacccca acaggcgttt ctaccagcct
      661 cagccgtatt cgccaggaaa ttttccgtcg cccggctgga ggaagcgggg atgccacgat
      721 catggggggc aaatatgtag atcgggagga ctatggttac aacaccgaac gagtagccat
      781 gtaccgctac tttttagagc cgcttggggt atcggtggac aagtggtcca tgggcgtgcg
      841 gggtcgctcg cgcacctata cctttgccga tgttttcgat gactacctga cgccttttac
      901 cgcaggaatt gaagccaacc tagtcgctga acccctggca ggtttaagcg tacccaccaa
      961 ttgctccagc ctgattccgg cggtaaatgc cacactagcc gccttgccag gcgtaaatga
     1021 tgtgcccact tacacggatg tacagcgcat attcaacaaa agctgcatcg agtgccacgg
     1081 tggccttaac tacccaccgg tatccacttt gggtgttggg tacttggatt tttcagaaga
     1141 cgaaaatgcg ccttttggac atcgcctgga tcgctcccac ggatttgcat ttggcttcgt
     1201 aaccaatacc cctgcaacaa gttacctcta ccagcgcatt accaacacca gcgaagactg
     1261 cccatccggc atgatgccct gtggcggccc tgcgctacca agcaccgacc aggagaccat
     1321 ccgccgctgg attgtcggcg gcgccccgct caccatcggc gacccccaca tcaccacagt
     1381 gaacggccgc aattttgatt tccaaagcag cggtgaattt gtgttgctca agggcattaa
     1441 cgaagaaatc caagtgcggc aaaccccctt cggcgccgag gtgcccatca ccgaccccaa
     1501 caccggcatc gccgcatgcc ccagccttag cacagcggtg gccttgaatg tgggcggcca
     1561 gcgcattagc taccagccca atatcagcgg cgaaccggac cctaagggca tgcaactgcg
     1621 catcgacggc aagctggtaa gcctcaccag cagcggcatt aacttggcca acggcgggcg
     1681 cattatgcgc accccggcgg ccggtggcgt acaggtggaa ttccccggcg gcagcgccgt
     1741 tgtgatcacc ccttactttt gggactacta ccaggtgtgg tacctggcgg tagatgtgcg
     1801 caacgcccgc agcaccgaag gcgtgatggg ctcggtggcc cccggcaact ggctgcccgc
     1861 cctacccggc ggcgacctgt tgggcggcct gcccgcaagc acaagcgatc gctttgatgt
     1921 gctctacagc aagttcgccg atgcctggcg ggtggatgat aaaaccagcc tgttcgatta
     1981 cgcccccggc accagcagca aaacctttat gctgcccggc tggccgaaaa acggcgccaa
     2041 aaactgttat gtgcccaaaa tcccgggcat agcggtgcgc gaaccggtta aacccatgga
     2101 cctaaaactc gcccagcagt actgcagcac ggttaaaaac cccgccctgc gcaaagcctg
     2161 cagccaggac gtagccacca cggcggaacc ggcctttgcc aagccctatt taaccgttga
     2221 gcaattgcag ctgaacaaca aaccgctgcc ggtggaattg gcctacccga aaaacaacca
     2281 aaccgatgtg agcgagaaaa ccaccttcac ttggaagcca accctggata aagacggcga
     2341 taaactcacc taccgcttct gcctgtgggc ctccggtgaa aaactcacct ttgaccgctg
     2401 caccctgttg ccggaacaga aagacctggg cttctttgta cctagcaacc taaaagtgag
     2461 ccaggcctat ttctggaagg tgattgccga ggacggcaaa ggcggcacca cagacagccc
     2521 cacctggcgc tttgcggtta aataaccgtt caatggcctc ctttaagcag cctgcgggct
     2581 gctttttttt tgcctggacg atttggccat ggcgcaatag gggccttggt acgcaacaat
     2641 tgaaactcgg tacatggaac ccggtagcct ggatgcagca cactccgggt ttttggatgc
     2701 ctcggattgc gctgcgctgc atccaggcta caaggagcgg caaatcccgt ggataacact
     2761 gcctattaaa cgtaaaggta atgtaattag gttgaggctt gtcggcgcct gcacgcccta
     2821 aaattcccct cctgtggagg ggtggcaggc ttgcctgccg gggtggttgc gctggagaac
     2881 cccgttctat ttcaggggac agggaaatct tttacccata atttctctta cccatatgtt
     2941 cagcgggctg cgaaccaaaa acagcccggc gatctaccac cccacccctt cggggcaccc
     3001 ctccacagga ggggaatttt aggccctgcc catgttgtac ctgcttaaca ccgatcacac
     3061 cgcgccatta attaacctgc aaacaggtta aaaacgccta tcattaaccc tgaaatctca
     3121 cgggctaatt gacagcgttg acacctgccg cccagaatca aacgccctca gcacagcctt
     3181 cgcgtcagca gaccatgccc ggctcatggg gcgcggttaa tgggtaccca ttggcacaga
     3241 cgacaggccg cacgggcaac aacactataa gaataaaaac cggttaaaaa agtaaccgcc
     3301 tcggccttaa gtgtgggctg aagcgtaggt atcggtaacc cggcgtgcca acccacagga
     3361 caggttaccc aggatcagtt accggcaggc catccgctgc cccagcccaa taggtaacca
     3421 tgaatacgcg agctcagctt actgaccaac ccacgcgcag ccccaccgac gggcgggtgc
     3481 cctcgtttac tcgcgccctg ctcaccaatg tggtcggggt ggtgattgtg ttggagctgg
     3541 tattgctgat cggcatggcc tggcaggcct ataccagcac ccatcaagcc gcccgcacca
     3601 aagcccaaga gacccaagcg cgggtgagcg agcggctgcg cattctgatc cgcgccgccg
     3661 agatgaccgg cgaatccgcc gagcgcgccg cccgcacccc ggaaatctcc tccgccaccc
     3721 tgcgcagcac catcgaacat tccctggcgg cctttgagca gcggccggag ctgagttacc
     3781 tgggtattgt gctggcggat aacggcgcct atggcaacct cgagcgcacg gccagcggcg
     3841 acattttgct ctggctgttc ccaggccagg acgccaaggg cgaagttatc cgcagcttct
     3901 tgcaaacccg cgcaggcttc aagccccata gccaattgcc gggctacgag tacgacccgc
     3961 gcacccggcc cttctaccaa gcggcggtca acagccccag cggagaaact tggatgccca
     4021 cctatcagtg gatcgtccac gaaaacacca gcgaatccct ctgggggttt agctatgtaa
     4081 aagccctgcg cagtgaagat ggccgcctct taggtgtgct cgacagcgac ttcgacctgc
     4141 ccgccctcaa ccgctttttg cgcaatttgg aacaggagta ccagacccat attcaagtgg
     4201 ttgaactggg gccaacccac cgcctgatcg gcgcggccag cctcaaacaa gaacccatgc
     4261 ccctgagcgc cgagcttagc gccctggtga agatcgccca ggcagactac gtcgctaaac
     4321 gcccccacgg gggcgagcac cactgggtgg ccgccaatcg gctgcgcctt aaaggcggtt
     4381 tggattggct ggtgattacc tccagcccca gcccctttat tgaggccccc ctactgcgcc
     4441 agctctacca agtaggggcc atggggctgc tgattgccct gggcttgatg tgggtatccg
     4501 tgcgcatggc ccggcggttt ggcgcgccct tggcggagct ggagcggcag gtgaaccaga
     4561 ttggcctgca ggagctgagc ctaaccaccc cgctaagtaa cacccgcgcc gcgcaatttc
     4621 gcgagaccca gttgctaggc caagccctcg accacctgcg cctggtggtg ggccagtatg
     4681 tggagagcaa agagcagcag gccgcctcac tcaccttaaa aagcgttatt tttgactcct
     4741 ccaaaaccgc cattttgagc ctggatgaca agctgcagat tctggaaatg aaccgcgccg
     4801 ccgagcgttt attcggccgc taccgggagc aattcaatgg caaggatgtg cgcaccctgg
     4861 tgcacctgaa cgacccggcc ctcagctggg agctgatccg cgccagcaaa ggcattaaaa
     4921 ccactcaatt taaaggcccc tgcggcgtgt ttgatgccga gtttcgggtg gccaacttca
     4981 aaagcggcga cacccaggta tgcaccctgt ttatcgacga catttccgag cgcaaacagg
     5041 ccgaggcgcg cattcgctac ctggccaccc acgacgacct cacaggcctg cccaaccgca
     5101 ccctgataat ggaccgcata gtacaggcca tccgccacgc ccgccgggcg ggctcccagc
     5161 tggcgatttt gtacctggat ttagaccgct ttaaggtgct caacgacggc tacggccact
     5221 cctttggcga tgccgtcctc aaagccgccg ccgagcgctt aggcgcactg gtgcgcgagg
     5281 gcgactcggt ctcccgccag ggcggcgatg agtttttaat cctcttaacc ggcctgcaca
     5341 gcgcagccga cgcctacata gccacggaaa aaatcgttaa acagctggat gcccccatcc
     5401 tggtggacaa ccgcaaggtg cacctctccg gcagcatcgg cgtcagcgtc ttcccccacg
     5461 acggcgacac cccggaaacc ctcatcgacc acgccgacgt agccatgtac cgggccaaag
     5521 acttaggccg caacacctac cagtttttca cccccgaaat gagcgcagaa acccagtggc
     5581 gggtgaactt agagcaccac ctgcgcgaag ccatcgcgga aaatcaattc accctcgcct
     5641 tccagcccaa ggtgaatctg gccagcggcc gcattgtcgg ctgcgaggcc ctactgcgtt
     5701 ggcgccaccc caagctcggc gccgtctcac cggaccaatt tatccccatc gccgaggact
     5761 caggcctgat tgtgcccatc ggcgactggg tactgcgcac cgcctgcgcc caggccaaag
     5821 cctggagcga caactgccaa aagcagccct gcatcgccgt gaatatctcc gcccgccagt
     5881 ttttgcagca ggacgtggcc agctgggtga tccgcaccct agaagaaacc ggcctgccgc
     5941 cgaaccaact ggagctggaa ttcaccgaaa gcctgatcgc caaggacgtg aaaaaagtaa
     6001 tcgccacctt cggccagctg cgcgccatcg gcgtcaagct ctccatcgac gacttcggca
     6061 ccggctattc aagcctcagc tatttaaagc gcttccccct ggacaccctc aagatcgacc
     6121 aatccttcgt gcgcgatatg ctcaccgacc cggaggacgc cgccatagtg cgggcagtga
     6181 tctccctggc ccacagcttg gaatttaacg tcatcgccga aggggtagaa accgaggccc
     6241 acaatgcctt tttgctgcaa aacgaatgcg acgaaattca gggctactac ttcagcaaac
     6301 ccctccccgc cgccgaattt gaggccctgt tggcggaggc aatgtaaagg cgttaggcac
     6361 aatttagccg cctcttgaga acgccatgtc ttagatgtag aggtagcaaa cagggttttc
     6421 gccagcagtg ccttaaattc ccctcctttg gaggggtgac ccgaaggggc ggggtggtag
     6481 gacgctatgc tgtttcttgc gcagcccgct gcgctcctgt cgataaaaaa taggtaaaaa
     6541 aatttgctcg tcccgtggca atactcaggg gccgccagcg caaccacccc ggcaggcaag
     6601 cctgcccccc tccaaaggag ggaaatttta gggcgcccaa acccatgcca ctgcccagga
     6661 tagaagcacc cgcgccaccc tgcaccccca gctcagaatt atttaacacc gcacactttc
     6721 cacccgccat aacttttcta tactccaacg gcagtgctga ttcagagacg aaaatagcca
     6781 cacctaggag gccagatgaa gtgcccaaca ctgttgcccg atgagcctga acgcctacag
     6841 gccctcgccc ggtacgggtt cgaacaaggg cagatgttaa aaagcctcga tcccgtcgtc
     6901 caaattgctt cccatatgtt tgccatgccg gtggccgccg tgaatatgat cggctcggac
     6961 catgtgtttt ttggggcggc agtgggcata gagtaccggg atctcagcac cctcgaacgc
     7021 agccgggaag tctccttctg cgcccacgcc atcacccaag atgaagtgat ggtggtagcc
     7081 gacgccagtt gcgatgagcg ttttttcgat aacccactag ttaccggcaa cgcccaggtg
     7141 cgcttttacg ccggcgttcc cctgcgctcc cccgaaggtc atgccctcgg cgccctgtgc
     7201 atcctggata ccaaacccca cccagaattc tccaccgcag atgctaagcg cctggcggag
     7261 ctggccaaaa tggcctccga ccggctggag ctacgccgta tcgaagtggc cagtacgcgc
     7321 tttcggccgc cctttgccag ctacgccgcg aacctgccca gcgccgtcat ttggtttgat
     7381 gaacacctgc aactgctgga gtggaaccaa cccgcagctg attttttagg ctttcccttg
     7441 gaggccaaac cgggcctcac ctttgacgcc ctcttggccc ccggcgcccg cgcgggcttt
     7501 cgccgcctga ttgccgacgc ctgcgcccag gcatcgctag aacaagtaca tttccccgaa
     7561 gaactcacgg ttctcggcgc ccatggccag gaaatcccca tcggcctggt gctgctcggt
     7621 tggcgggaag cgggccgcat gacctttgaa gtcatcttta aagacaccag ccacgaaccg
     7681 acccacacag gcctggatga tctcaccggc cttgccaacc gtggcacctt ttaccgccgg
     7741 gtagaaaacc atctgctgca atcccccccg gacggcgcgg tgctggtcat cgacctggac
     7801 ggctttaaag accttaacga caccctcggc cagggcttgg gtgatgacat actgcgagcg
     7861 gtggccgagc ggttaagcca gagcgtcgac agcggcacct tagtcgcccg cttgggcgcc
     7921 gacgaattcg ccgttctgca ccctcagccc ctcacacccg ccgccgccct cagcctggcc
     7981 caacacctca ccctcgccct cacccaaccg ctcagcctgc gcggccagga cgtgcgtatc
     8041 agcgcctgct gcggcttggc cctcgccccc caacaggctc aggaggccat tgagttggtg
     8101 agcaacgccg acctcgcctt gtttaaggcc aaaacctgtg gcccaggccg cacccaatta
     8161 ttcgacccca acctgcgcca agaggccgtc acccgccgcc tctacggcct agaactgcac
     8221 cgggcggtgg ataaaggcga gtttttgctc ttctaccaac cgcaaatcaa catggccgac
     8281 ggcaccatca ccggcgctga agcactcatc cgctggcggc acccagagcg cggcctatta
     8341 agccccgcaa gttttattca caccctcgaa aacggccccc tggccgccac cgtaggctcc
     8401 tgggtactta acgaagcctg cgcccaagcc gcccactggc ggcgcatgtt taataatgac
     8461 tttcgcatcg gcattaattt atgcggcgcg cagttttacg gcggcagcct caccgaggac
     8521 gtgctcgcca ccctcgcccg ccacggccta ccgcctgcgg ctctggaatt ggaagttacc
     8581 gaaaacatcg tccttgccaa cgcggatcta attcttgatc acctacaaca actgcgcgcc
     8641 cgcggcatcg gcatcgcctt cgacgacttt ggcaccggct acgcctcctt aagcctactc
     8701 aaaagctacc ccttatcccg tattaaaatc gaccagagtt tcgtgcgcgg gattgccacc
     8761 tccaaaggcg acgcctcagt ggtgcgggcc attttagata tggcccgcaa tttcgaactg
     8821 gaaaccatcg ccgaaggtat agaaaccagc gaagaattag actttttaca gcgcgaacac
     8881 tgcaccgagg ggcagggtta tttatttggc aaacccatgc ccgcgtggca gtttgaaacc
     8941 ctgttggcgg cggatcggca ggcggatatt gcttagcatt ccgaggcatt tagcttcact
     9001 taggtatttc aaccaactag atgcaaaatg atcttcagac cattcattac ctagtcttcc
     9061 ccttccaagt tttgtcagcc atgttctgag tgacacagcg acgaaccttt aaaatctcta
     9121 atagaaatca accgattgag gtatggcaag tgagctgaac aatgaacctt agaacattaa
     9181 acaatcaaat tagacgttca gaagccaaaa tagccattca actagccaag aataattgcc
     9241 acaacaaaac acaagagatt aattttgagt ggctcattac ttgggctatc aagaaagcaa
     9301 ataaaaaaac accattgtgg tgcggcaatg taaaaattat gtctttatac aaaaccgact
     9361 caagacagtt ttacattacc gcaacaatcc acattggctc cgaattagac accagcaatc
     9421 aattagctga agcgacactc catggccaca tgctcctaaa tggtcacaaa aatcgtttga
     9481 agagttacct gctcacaatc acacacaatg atgaagaata caaactacac aaacctgctt
     9541 aatcgacagc attaactaca aaattcaata ccgatacacc ttagagagaa gctgaaacaa
     9601 cacccggaca atactcggca aatcaacacc aattattcca atagaataaa tacttctccg
     9661 agcggcacta ccgctaaaat tttttcctat agttatactg catcttttta tgtgcctgga
     9721 ggtaacttcc gcactgactg gaaactatcg caggtgtgtt ttagtgctgc gagccacaat
     9781 actccactcg tagtgattcg attctattac agaactaaac ccaccccaat atatctccca
     9841 cgcaggttga gcaaaaaggt ttgctgaaca tttactaatt cgtcacccaa tagaacgact
     9901 accaactcgt aacagaccct tactgttcag cgcaaactca aacggtattg atccggcaag
     9961 gcaaattaat aaggctgcaa tagccatgta ataaatctgg tgtataaacc tgtcgcaaag
    10021 tggatgcggt atcgggtgca acatgacaat aaaaaatata aaaatcttac tcgccgtatt
    10081 attgctgtcc cttgagggta ttgcagagtt gccttgcgca gaagcgacca ccaatgtgtt
    10141 aggcgccagc ccacagcggc acgaccgttt ttatcagggc gccgataaac aatttatact
    10201 gcggcagtac gatttatctg gcatcggaaa agtcgtcagc gcatccagca agtgggccac
    10261 catggtgagc gaacgctttt ttgtgtcgtc taaccatgcc caccccagca gcggagaagt
    10321 ggtgcgcttt tatttcagca acaatcccgc cgatgggttt gttgatcgca ctgtagccgg
    10381 tggcgaacaa atcaaagaca gtagcgggcg agcagtgggc gatctttggt tggggtattt
    10441 ttcggcccct gtgcccgtca atattgcgct gtatccgctg ctgggatcag gcatggccta
    10501 tccacccatc gacagtaaat taattctatt gggcaaggcc aatgatggct tgcaagctga
    10561 actcaacccg ttaaatcagc gcgtgggcag gaatatatgt gtagcccacg aaatcgctaa
    10621 ccacgcctat ttttatacct atgacccaat aggcgcggcc gattcattag gcgttgatga
    10681 aaattccgtg gtcgccgggg attccggcgc ccccacattt atcctgatcg ataataagct
    10741 cgtattagtc ggcacccgct gggggtttga tgccagctta aaggtggata tagattccag
    10801 cgtgattgcc aaaaccaccc ccctggccca ggcaattgcg cggctaagcg ccaatagtga
    10861 gcaattgcgt attttaaata ttcctgcacg attaactggc gccgttttat tagaagattt
    10921 cgaagcggaa caattaggcg caacaacatt aatcggcagc cctaccacca cgaaacacct
    10981 cattacaggt actgatgctg ggggccgcgc ggaggtagtc gcacgcaata acaaccaaca
    11041 actaaaactc acctccccta gtgacgccga taccgatagc ccaaaattat tttttgagac
    11101 aattgccacc gccataagcc aaggtgaaat ttcgctgcgt tttcgcattg acccaattag
    11161 ccaacagcct ccagttagca ataaacccat cttaaccctg cgcgcaggcg atgatattgg
    11221 gctaaaccaa atagccctcg acgggccaat tgcccaagcc gcaattcaaa tagaatttac
    11281 cgatacgggc catttaaata taacaactgc agacggcagc ttattatccg agcaacaatt
    11341 caccttcggc caagagcact tttttgccat taaatttaat ttaagcgcaa aaaactttag
    11401 cctttatctt gatggtgacc cgctgagtgc aaacagcgta aaacaattta ccccaataag
    11461 tgcggtaaaa aagttagatg ctatttatgt ggccagcagg ggtttgccca ataccagtac
    11521 cacggcctac gtagatgagc tgcatattga tgcaccaggc ggctggcgca gcctagagca
    11581 aacactggcg ctacctgttc tgggtgctaa tttagaaaat gacattttta gtagcaatta
    11641 ttggcagcgt gttgccaaca caactaattc tattttggat gtgggtgcag gcaaggcaaa
    11701 tgccggtgct tggcgcggtt tttcccgcct gggcatcaat ggctttaaca gctccgaaat
    11761 tgcacaaatc atcgacttaa cagcaagtgg cttaaacaaa gacatcgata aaggaggggt
    11821 tacagccacc tattctgccg caggcatgtc cgagcaaatt ggcgattatg cgcatttagg
    11881 catggagttt ttaaatgcag ataaagtgtc aattgaccag gcgccgcagg ttgccattaa
    11941 tgatggcaaa agccgctggg ctccattaca gcttcagcat ctggttccgg cggctacacg
    12001 gtatctaaaa ttaacagcag gtgttacgcg cagcgcagga attaccacag atgcctatat
    12061 agacggatta ttttctgcca acctgtcctg ggttaaaacc caagccattc tcccagtgga
    12121 tgtacaagcc cgcgacctag agcctgctga gctgattaat caatattggc agaagctcag
    12181 caacaacacc ggtgttttta tcgaatttgc caatgtcggc atcaacaaca ccggcacaag
    12241 tggcgtggcg aggggtggaa tatccggatt taaccgcgct gaactcgccc aaaccctcga
    12301 tttaaccact tccccctacc ttatcgatat tgatgcgggt aaggtattta tcaacgcctc
    12361 tgccttgggt atttcatatc aagtgggcga ttatgcgcag cttaaattag agtttttcga
    12421 taacacaggt attttactgg caacctcgcc catagtggca gttaatgatg ggcagggcca
    12481 atgggctgct atgaagatta ccgaatataa ggtgcccata aaaacccgta aagtgcgctt
    12541 ggtgattggc cttacacgct cttccggcgt cactacagac gcctacatag atggcccgat
    12601 aaaagtattt tttagccgcg cctaacctat taacccggcg gaatttagtg ccgagttaag
    12661 aatggcggca aggatgccgc gcaacaccat caatacacgc caccaactca tcctgcccca
    12721 aattatttcc cgcccctgcc aaagccgcgc agcgctgttc catggcgata tcctgcaggg
    12781 cttgtcgcac gagtgcttct atttgcgcgc ccggccaatg ggggtttaat acatagccta
    12841 aatggcggct ggctagggct tcggcggtaa ataattgctc gtaatgggtg ggaattaaaa
    12901 tggagggttt accgcacacc agcgcttggg aggccatacc gctgcctgcg tggtagatta
    12961 accactccgc ttgcggcatt aattcagtta aatccaccgg cgctaaataa atctgtagcc
    13021 gctcggttgc aaatgtctgg tagtgctcag cttgagcgcc actgatataa accaccgcca
    13081 ctaccggcat ggccgccaat aatgtcagca aatattcaag gcctgtgtat tccgttttta
    13141 aataggcaaa aacgcgcttg ccctgccccg caggccaggg cacggctaat ccaccgggca
    13201 tttgtgtggc tagaccataa tactgagtat ccgccgggcg cccctgggca aaatgatcaa
    13261 gctcctgggc gtgggtgata gccacctgag ctgtttgcca aaataaatcg cttacacaat
    13321 ttaataatgg agcgttaaat ttcgccaaat aacgatttaa cgccgctaat aattgctcgt
    13381 cggaggcttt taaggcggcc tcttgaatca ctaagcggcg gttaaatgcc ggcaacggcg
    13441 ataccggcgg cggcacgcta aaacctgtgc ctaataccac agcgggcagg cctaaactgc
    13501 gggccgctaa taagggtgta ggcgcataat cggccaggat taaatcggct tttaatagcg
    13561 ttaataattg ccgccacaca tgggcgcaag gggttaatac gctgtcggtg ccaagccctg
    13621 cacacagcaa tacctcgctt aaattgactg ggtcgcggga ggttttaaag gccggcatgg
    13681 gcaatttagg ccccaataca taagcgatgg gcggctcttg ggcctgcata aaccccacaa
    13741 aggaaatatc cggcagcacc gccaccactt catggcccag tgcatgtaag cgttgcaata
    13801 aagaggttat tttactgata tggcctaaat aaccacccag ctcacagata ccaatgattt
    13861 ttgccacagg tcgattccgg cattttaaaa atccctgcgc gtctgaagtg caggcataaa
    13921 aaggtttgtt gcaggatgtg ctttacaaac ggcataaaaa aacccggatt tcgcttgcgc
    13981 tccatccagg ctacttgctg cacataatca actgcgatta gctgctttaa aactccatgc
    14041 gcgcgcccag ggcgtagtga tcagacgacc acttatcgcg gccaaattgc ccggtataac
    14101 ttaaataagc cgatacaccc tgggtaaata cggcgttgag ggccaggttg ccgttaaaat
    14161 agttggcgtc tttatcaaag gtggaaatgg aaaattcacc agcttcaccg ccgataaact
    14221 ggccgtccac attaaagcca tcacctttaa attggtgctc ataatccaca cccaattctg
    14281 ggcttaacac accaaagctg gtattaataa tccaggtgta gctcacgcct aaattactgg
    14341 taaaggattt aatggaaaaa tcgttgattt tagccaccag catggtgtca tcaccagaga
    14401 attccgcata actatccacc cccacatcga tataatccaa gcgggtgaat aattttaact
    14461 gcatggtttt aaaatcaaac tgataacccg cgcccaaacc aaaactggtt tcactgccgt
    14521 ccgtatcggc attggccaag cttttaaccg cagcgccgtc taatacgctg cgcaccatgt
    14581 cgtaattatt tttaccatag gtggcaaggg tatcgaggta aaaattatcg gcgtagaagc
    14641 tgccgaaaat gctaaggaac ctctgaaaaa ttactcaaaa ttggtaaaat ggcacctttc
    14701 ctaccgccgg tgaacattat gagtcaatta acctttgccg aagcagaata cgccatcaaa
    14761 aagcgcacca ctcgccggg
//
LOCUS       DAOQGS010000024        14625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1893_length_14625_cov_5.621757, whole genome shotgun sequence.
ACCESSION   DAOQGS010000024 DAOQGS010000000
VERSION     DAOQGS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_14625_cov_5.621757"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKZ52_01705"
     CDS             <1..771
                     /locus_tag="PKZ52_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58508.1"
                     /translation="AQVGETYNWQQWTGTDLVLINNTKRDSAQIEFKVPGQRTSVPLI
                     LLRQTTTPDSERQDIFVIYRIGDCRLPYGVVFQDCLGNDFGPLKVSGRVTFGTTFYTF
                     PITEISQSSFSIEANPIYWREVLSSDASHAQVIDVTHRLFTGSSTYSGSFFIPFAEQQ
                     LVNWQAFAGGSLEFDLNIIDYGDSGEAITVSSGLIQHALSPQPLGLWRHISVPIPAAE
                     IWLSPLANDAPFGIFAQNQIRGGLHYQIDNIQFKKAAD"
     gene            complement(844..1746)
                     /locus_tag="PKZ52_01710"
     CDS             complement(844..1746)
                     /locus_tag="PKZ52_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNG58509.1"
                     /translation="MKPASAVLIVNKKARSGAELDIAMCLAILQAGGISAHVFSLGED
                     ELWQLDEWMAEADVLIVAGGDGSINHNLTRVIEAGKVLAIIPMGTANDFARNLGVPND
                     ARLACELIVAGNVAQLDVASVNGHHFVNVAHIGLGVSVTYELNDEVKKRWGILSYLRA
                     FISALKRMRTFRAKLQLDGRYKRYRLLHLSVGCGRFYGGGNVVDEHCYANNGWLNITG
                     LRKRSLFKLLISLPFIRFGRHRLISEAIVAKAKRLELTTRRPMEIHADGEPVGFTPAV
                     FELQRKALRVIYNVQEHHLMLSDA"
     gene            complement(2357..2872)
                     /locus_tag="PKZ52_01715"
     CDS             complement(2357..2872)
                     /locus_tag="PKZ52_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58510.1"
                     /translation="MSRIEAFLPTQTLLTLVAELAEKEGANIIFCRYKPTDQKPGFTV
                     VQAAPDLANKYLDGKYEQILLSLKGLPKHEQDWEFIERASAYLIEINGGRQDADNIGQ
                     TTIRLIDKKSKATKVFNGLKAAVKAISGVGVLVNGNPYPKIYYAQSLIASCPNLWVDI
                     ETKQLAVKLPG"
     gene            complement(2869..7065)
                     /locus_tag="PKZ52_01720"
     CDS             complement(2869..7065)
                     /locus_tag="PKZ52_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNG58511.1"
                     /translation="MMPIIKHFDPVMGIDIHIVAIPPVGPVPIPHPHIALVLDPMDYI
                     PIIGATVLVGGIPRGTAGTSGKVIPHIPMGGPFVKPPMNEDEIFMGSATVLADGSPLS
                     FTALPVLGCQDVGMISPIRLKKPKKTYGMVLPSSIVMGIPAGMPVLVGGPPTIDMMAM
                     AMKVGFAALGKAMKKLRALQKRSARFKKLSKAVHERAKKIMDKLGVPPNIRNKVHKAI
                     CTVTGHPVDVAAGKLFTDAVDITLPGPIAFKWERTWFSTSIYQGPLGHGWHHNYDLAL
                     MQENGALAIRLSDGRPVAFPILTGDEVSFNRDERLWLSRDGGGQGDGYALKTSDGDIY
                     YFHPSKLRPGLFNLTAMGHKAVFERILFYYDTNNRLSQIVDCAGRIIQLEYNRFNQIA
                     KIHLPHPNEVDKTFCAVQYYYENDNLVRVDDALGQPWSYAYQNNLLVQEIYRNGLNFY
                     FRFDEYSPAGRCIETWGDEGIYYRSIRYDLDNNITYVRDSLGYVSEYHHDLVLPHKIV
                     DPLKNVSSVQYNSHGQAVCEVDALGYKTLYEYDELGNIIQTTHADGSITRFAYDSRQN
                     LISLEELDGCPWYFEFNEFNQLSLEIDPLGNKTRFSYAGNQLYCITDPAENHYFFNYD
                     QQLNLIAVADGQGKQIQWQYDGLGNLLIATDPMGNQRQFTHDLLGRVIQVNEPDGNLR
                     TLKYDGLDNLILMHDRHSDVQLSYKGLSCLVARSQGGKQVNFRYDTEEQLIAIANEHG
                     QVFRFELDPNGAVTAEEGFDGLMRRHIRDPLGRVHKTLRPDQRFSIYQYDAMDRIIEV
                     HHHNGEQEKYSYRADGELMAASNSACTIEFERDALGNTLKEIRDGKYWVMSQYDALGY
                     RCALSSSMNFAQTSVRDKQGNLTQVGIANSNAEYIAAEYRRNELGLEIQRTMPGGIKS
                     RWQRDKFGRPVEHVISSGHKTHSHKTYLWGMDDRLLKIVESLKRETNFQHDILGNLTA
                     AHYNEQDFDVRLPDAVGNLFKTQSQRDREYGPAGQLLSKQEKDGITRYQYDAEGNLII
                     KYLPDGKSWRYSWSATGNLAKVIRPDGSEVCFTYDPLGRRISKTYRGKITHWVWDGNN
                     PLHEWVEWLNPRAKAADAPSLYKAPLQQQTDAVLQPLQPQAPPRLHAGSSDQPATWVF
                     EPESFTPMGKFVGNNFYPLVSDYLGVPVAMFDPDGQKIWSAEISLWGEMKNLTGDLHT
                     CPFRWPGQYEDEETGLYYNRFRYYDPDAGSYISQDPIGLLGGLQLYSYVGDPAAWIDP
                     WGLTKQCGPGTQRWKNKRTKSGRPYKKPGPKKSPSAPHNKKIREIIRQEKAKGKIHIG
                     GGSKTELIINTSGGKKPYRRMDASFRDPVTGNIEHHNVGKSNRGNGQPIARERDAISD
                     VQNHAPPADRDITFHSYGTT"
     gene            complement(7062..8393)
                     /locus_tag="PKZ52_01725"
     CDS             complement(7062..8393)
                     /locus_tag="PKZ52_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58512.1"
                     /translation="MNAIEQEVTILNQHWDEFILGEQAILHWLINPADTLLVNALVKI
                     KEQFDEESGTWFIQLTSPFATAAGYGYTLAEEFNRLIEQGLAEALEEEAQEETARETA
                     EAPELINAWQAPDLNTAQSGLHALFVSIERVLALFDKSLEQLTLVINPTGIKSLTEYC
                     LWWQQACHIARSYSTWPGKLKLLALDNGAQPSLTPVFAGFTDVAKTQTPPINLNAAAR
                     AVAEQTHDGTDSAQLRLLFLQMNAAITEQNSAELNSAAEAALEITRSNQWPDMTATVL
                     LTRAGGLLNFQQFPAAIIEYQQAQLFARMGIENAVPGCDKLLLQALLFEGTANFLAQH
                     LNQAVIAYEKAAQQAALLNDEWIALEAWRMAAFCSERLNQTERAWEQANFALAVGKNM
                     APEIRAQSTLGFVGQAMLRLKSNPQMQKDINATFSELLSSDWLEKLEGAAA"
     gene            complement(8445..10586)
                     /gene="tssI"
                     /locus_tag="PKZ52_01730"
     CDS             complement(8445..10586)
                     /gene="tssI"
                     /locus_tag="PKZ52_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNG58513.1"
                     /translation="MSLGFTPAALFTQETRHLRIQTGLGNDVLLLRRLSGTEGISQLF
                     QFELDLLSHDGAINPLNIVGDNVSICIQPGEGAARFINGFVKSFQYCGLEKRGLYAYR
                     AEVVPWFWFLSKRTDCRVFQNKSVKDIVEFIFKELGFFDYVFALMEVHKPLEYCVQYN
                     ESDLDFICRLLEQEGIYYFIEHQPDKNILHLSDSPHHYGDCVPTSAEHSSGIRRDEYI
                     RQWQHNYEYCSGAFAQTDFDFEKFTQSLLTQTATHLKLKNNSSFPQFAYPGSYRESDQ
                     GTALTKLRMQQEELGHERAHGASNIHSFEVGKKFTFKSDEAQADHNKSYVITQIQHTA
                     YNPSFLESEAQDPPYANQFVCIPAQTPFRPAFNTPRPRIDGVQTAVVVGPAGEEIYTD
                     QYGRVKVQFHWDRYGKKDETSSCWVRVATQWAGSKWGSITIPRLGQEVVVTFINGDPD
                     QPLIIGSVYNSANTPPYSLPDNKSMMGMKSRSSKGGDGGTYNELVIDDKKDAEEFRIN
                     AQKNYNMKVGKDASRHTVGNTTTKVDGNASTTVAGDTSHSTGGNDSFNVTGNRTAGVK
                     GNQDSTIQGNSTQTIVGNSDDSINGNSTLTVGSNNTQSIGANQELSVGTNQTVSIGVN
                     QETTVGANQTIQVGGKQDISALAQSVSITTNAELSALQISQSGTAQISLSVGPSSITI
                     TPAGITLSMGASSVSINPAGVMVSGPIIKLN"
     gene            complement(11238..11507)
                     /locus_tag="PKZ52_01735"
     CDS             complement(11238..11507)
                     /locus_tag="PKZ52_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG58514.1"
                     /translation="MHERREHPRIPLRVNIKIAHPDFGEKVVVTRNFSEGGLFIIVKP
                     TDLPPPGSIVKGQIQDMPEDAPVVDMRIVRVEKDGVGLQYLVDTL"
     gene            11656..12021
                     /locus_tag="PKZ52_01740"
     CDS             11656..12021
                     /locus_tag="PKZ52_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58515.1"
                     /translation="MPIRLLRPLCCLLVVSLSGCIKLHLLPEDSIQHSVDAGKNLIDQ
                     AKLARAGGKKMTLASEVKLSAAATQKAAEQACLAELKTRAAQASSKRAYELVSEKTHL
                     GKTDGIEIIGCEILIYVWN"
     gene            12364..12663
                     /locus_tag="PKZ52_01745"
     CDS             12364..12663
                     /locus_tag="PKZ52_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58516.1"
                     /translation="MKNIPSLSTVLASAFLASASLSVPAQSADFAIQPLAQGYQLAEN
                     AAAKPAEGKCGAATEGKSHEGKCGAEHKDDGEKKPEGKAKEGKCGEGKCGANKKG"
     gene            12900..13760
                     /locus_tag="PKZ52_01750"
     CDS             12900..13760
                     /locus_tag="PKZ52_01750"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNG58517.1"
                     /translation="MPNLMPNKLPSLKPQALWQGAGLGLHARHLPELLATPPAAVEFM
                     EIAPENWLTKGGQSARQLQALAERMPLVAHGLNLNLGGRRPLDKELILAIKGLHHSLN
                     LRFYSEHLSWCADEGNFYDLLPLPFTAEAVLHVAARIRQVQDMLECFIGVENISYYLP
                     QNPGDLSEPEFITAVLQEANCLLHLDVNNVYVNSINQGMDARAFIDAMPSEKIAYMHI
                     AGHSQEAADLIIDTHAAPVADPVWDLLAYTYATKGVVPTLLERDFNLPPLAELNQEVA
                     RISCVQHQYA"
     gene            13773..14516
                     /locus_tag="PKZ52_01755"
     CDS             13773..14516
                     /locus_tag="PKZ52_01755"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNG58518.1"
                     /translation="MQPPIYLPCRTSVLSLAEYQADFAEGLRSDSSKRQQAYNGRLGL
                     YKKLIFANLCSFIDSCFPVCRSLLSPAVWRQLCRAFLREHACQSPLFHQIPLEFISWL
                     QMPHRFRHNLEPAYLTHLAHYEYCELQIQTAPDINHYAKPNNCALFITPIQLVCYPYP
                     VHTIGPKNPAPALATTRLLLWRKADDSAGFAEITANIYCLLELLQHKQDLAEIFTHWA
                     PLCTPAASLDEFVASMQNLATLGVIEIHP"
BASE COUNT         3732 a         3686 c         3399 g         3808 t
ORIGIN      
        1 gctcaagtgg gtgaaaccta taattggcaa caatggacag gcactgacct cgtattaata
       61 aacaatacta agcgggattc agcgcaaatc gaatttaaag tgccagggca gagaacgtcg
      121 gtgccgctca ttctactcag gcaaaccaca acgccagact cagaaaggca agatattttc
      181 gtgatttatc gcatcggcga ctgcaggctc ccttacggcg tggtctttca agattgtttg
      241 ggaaatgact tcgggccgct gaaagtatca ggacgagtta catttggaac aacattttat
      301 acatttccaa tcacagagat ttcccaatcc agtttttcaa ttgaagctaa tcccatatat
      361 tggcgagaag ttctcagcag tgatgctagc cacgcacagg taattgatgt aacccaccga
      421 ttatttacag ggtcatccac ctacagcgga agctttttta ttccctttgc cgaacaacaa
      481 ttagtgaatt ggcaagcgtt tgcagggggc agtttagaat ttgatttgaa tatcattgac
      541 tacggcgata gtggtgaggc aataacggtc agttctgggc ttatacagca cgctctatcg
      601 ccgcaaccac tggggctgtg gcgccatatc agcgttccca ttccagcggc ggaaatctgg
      661 ttaagtccgc tagcaaatga cgcacctttt ggcatatttg cacagaatca aatacgcggc
      721 gggctgcact atcaaattga caatatacaa tttaaaaaag cagctgacta agttattcac
      781 aataaaatct cgcctatcgg gcgtttgctt gaaggtcgaa ggattgactt ggtgcagtaa
      841 cgcttatgcg tcgctcagca tcaagtgatg ctcttgcaca ttataaatta cccttaatgc
      901 cttacgctgc aattcaaaca ccgcaggagt aaaccccacc ggctcaccat ccgcatgaat
      961 ttccatgggc ctgcgggtag taagttcgag gcgctttgct ttggccacta ttgcctcgct
     1021 aattaatcga tggcggccaa agcgaataaa cggcagggaa ataagcagtt taaataggct
     1081 gcgttttctc aaacctgtga tattaagcca accattgttg gcatagcagt gttcatccac
     1141 cacattaccg ccgccataaa agcgcccaca accaacggat agatgcaata agcgatagcg
     1201 tttgtagcgg ccatccagtt gcaacttggc cctaaatgtt cgcatacgtt ttaatgcact
     1261 aataaacgcc cgcagatagc tcaatattcc ccagcgtttt tttacttcgt cgtttaactc
     1321 gtaggtaacg ctcacgccca gcccaatatg ggccacatta acaaaatgat ggccattgac
     1381 acttgccaca tcaagttgcg ccacattacc cgccactata agttcacagg ccaagcgagc
     1441 atcattaggc acgcctaaat tgcgcgcaaa atcgttggct gttcccatgg ggataattgc
     1501 caatactttg ccagcctcaa ttactcgggt taaattgtgg ttaatactgc catcgccgcc
     1561 cgccacaatc agcacatccg cttcggccat ccattcatcc agttgccaga gttcatcctc
     1621 gcccaggcta aatacatggg ctgagatgcc tccggcctgt aaaattgcca aacacatggc
     1681 gatatctaac tcggcccctg agcgggcttt tttattgacg atcaaaacag ccgaagcagg
     1741 cttcataaat actaaccatt ttaatggtgg ccctgcttgc taaagggcca gaaataagcc
     1801 tgcacaatac atatttgcac atgcacatgt atagatatat ttatagacgc caatgctagc
     1861 agcttattga aatacgtcgg ggcgacacgc tggcaaggta aatttggcga aaaacccgga
     1921 cgaatgcatg aatacaggag gtagagtaat gcaagagcaa ttgccgagtt gacacgcagc
     1981 aaatgaggat tttttcaaaa tttaacgccg cccacactcg cacggtagta tttcaacaag
     2041 ctgctagggt attaattttt aatgggcctg tatttatgct gaagcttacc actggcacaa
     2101 gcagattttt atagcgcgcc gtctcgacat cgaatagcgg tgtgtatagt tcggccaaat
     2161 agtcacttat attattgcct aatgtattat tgcctaatgc gatccctgca cgggatacgc
     2221 caggaatcat tggcagcgaa aatcaatcat agggtttgtt gcttactctc ataaatcaca
     2281 catgcagccc cgcacttccc gctaaatggg ctttctcagt ttccccgtgc ctgaatttaa
     2341 ttagaggcca ccctattcag ccaggcagtt taactgccaa ttgtttagtt tctatatcca
     2401 cccataaatt tgggcaagag gcaatcaagg actgcgcata atatattttt ggataggggt
     2461 tgccattaac taaaacaccc acaccactga tcgcttttac ggcggcttta agtccgttga
     2521 atactttggt cgctttactt tttttatcaa ttaagcgaat agtcgtctgc ccaatattgt
     2581 ctgcgtcctg gcggcctccg ttaatttcaa ttaaataggc cgatgctcgc tcaataaact
     2641 cccaatcctg ctcatgtttt ggcagccctt tcagggaaag caaaatctgc tcatatttac
     2701 cgtctaggta cttgtttgca aggtccggcg ccgcttgtac cacagtaaag cccggctttt
     2761 gatcggttgg tttgtagcga caaaatataa tattggcgcc ttccttctcg gcaagttcgg
     2821 caactaaggt caagagagtt tgggtgggta ggaatgcttc tattctgctc atgttgttcc
     2881 atacgaatga aaggttatat ctctgtcagc tggtggcgcg tgattttgca catcgctgat
     2941 ggcgtctcgc tcgcgggcaa tcggttgtcc gttgccgcga ttacttttac ccacattatg
     3001 gtgctcaata ttgccggtaa cagggtccct gaaggatgca tccattctgc ggtagggctt
     3061 tttgccaccc gaggtattaa tgatcaactc ggttttagag ccaccgccga tgtgtatttt
     3121 acccttagct ttttcctgac gaataatttc ccggattttt ttgttatggg gtgcgctagg
     3181 actttttttg gggccaggtt ttttatacgg gcggccagat tttgttcttt tatttttcca
     3241 cctttgtgta cctggcccac attgcttggt caatccccaa ggatcaatcc aggccgcagg
     3301 gtcgccaaca tatgagtaaa gttgcaagcc gccaagcaga ccaattgggt cttggctaat
     3361 atagctgccc gcatctgggt cgtagtaacg aaaccgattg taatacagcc cggtctcttc
     3421 atcttcatat tgcccaggcc agcgaaatgg gcaggtatga agatccccgg tcaggttctt
     3481 catctcgccc caaagggaaa tttcagccga ccaaattttt tggccgtccg ggtcaaacat
     3541 ggcaaccggt acgcccaaat aatcactcac taatggatag aaattattgc caacaaattt
     3601 ccccatgggc gtgaatgact caggctcaaa tacccaagtg gcaggctggt ctgaagaccc
     3661 tgcgtgaagc ctgggcggcg cctggggctg tagcggttgc aacaccgcat ccgtttgttg
     3721 ctgcaggggt gccttataaa gcgaaggagc atctgcggcc ttagcgcggg gatttagcca
     3781 ctctacccat tcatgcaatg gattattgcc atcccacacc cagtgggtaa ttttaccccg
     3841 ataagttttg ctgattctgc gccccagtgg atcataggtg aagcacacct cgctgccatc
     3901 cgggcgaatt accttagcca aattgccggt tgcactccag ctgtaacgcc aggattttcc
     3961 atccggcagg tatttaataa ttaaattacc ttctgcatca tattggtacc gagtaatacc
     4021 atctttttcc tgtttagaaa gcaattggcc agccgggcca tattctcgat cccgctgaga
     4081 ttgagtttta aataaattac ctaccgcatc gggtaagcgc acatcaaaat cctgctcatt
     4141 gtagtgtgca gcggttaagt tacccaaaat atcatgttgg aaattcgtct cacgtttgag
     4201 cgattcaaca atttttaaca gccggtcatc cataccccaa agataggttt tatggctatg
     4261 ggttttgtga ccactggaaa tcacatgctc gacaggccgg ccgaatttat ctcgctgcca
     4321 acgactcttt ataccgccgg gcatagtgcg ctgtatttct agccctaatt cgttgcgcct
     4381 atattcagct gcaatatact cggcattgct attagcgatg ccaacctggg ttaaattgcc
     4441 ctgtttatcg cgcacactcg tctgtgcaaa attcatggat gaactaagtg cacagcggta
     4501 tcccagggcg tcgtactggg acattaccca atattttcca tcgcgtattt cttttagggt
     4561 attgcccaaa gcatcacgct caaattcaat cgtacaagcg gagttgcttg ccgccatcag
     4621 ctctccatcc gctctgtagc tatatttttc ctgctcgcca ttatggtgat gaacctcaat
     4681 gattcgatcc atggcatcat attgatagat tgaaaagcgc tgatctggcc gcaaggtttt
     4741 atgcactcgc cctagcgggt cgcgaatatg gcggcgcatc agaccatcaa aaccttcctc
     4801 cgccgtaacc gcaccattag gatccagctc aaacctgaat acttgaccat gttcattggc
     4861 aatagcaatt aattgctctt cagtgtcata gcgaaagtta acctgcttgc ccccttggct
     4921 gcgggccacc aggcaactga ggcctttata gcttaattgc acgtcactgt gacggtcgtg
     4981 cattaaaatc agattatcca aaccgtcata tttgagcgtg cgaagattgc catccggctc
     5041 attcacctga atcacccggc ccaataaatc atgggtaaat tggcgttgat tacccattgg
     5101 gtccgtagca ataagcaaat tgcccaagcc atcgtattgc cattgaattt gcttaccctg
     5161 gccatcggca acagcaatca aatttagctg ctgatcatag ttgaagaaat aatggttctc
     5221 agccggatca gtaatgcaat acaactgatt acccgcataa ctaaaccgcg ttttattgcc
     5281 caaggggtct atttctaggc tgagctgatt aaattcatta aattcaaaat accatgggca
     5341 accgtcgagt tcttcaaggc ttataagatt ttgcctgcta tcataagcaa aacgagtaat
     5401 gctgccgtca gcatgggtag tttgaattat attgcccagc tcatcgtatt cgtagagggt
     5461 tttataccca agtgcgtcaa cctcacacac tgcctggccg tgggaattat actgaactga
     5521 ggaaacattc ttcagcggat ctacaatttt atgcggcaac actaaatcat ggtgatattc
     5581 actcacatac cccagcgagt cgcgcacata ggtaatatta ttgtctaagt cataacgaat
     5641 actgcggtaa taaatccctt catcccccca ggtttcgata cagcgcccgg ctgggctgta
     5701 ttcatcgaag cggaaataaa aattcaggcc gttgcggtag atctcttgca ccaacagatt
     5761 attttggtag gcatagctcc atggctggcc cagggcatca tccacccgca ctaaattatc
     5821 attttcataa taatactgta cggcgcagaa ggttttatcc acctcattcg gatgcggcag
     5881 gtgtattttg gcgatttgat taaaccggtt gtattccaac tgtattatgc gcccggcgca
     5941 atccacaatc tggcttaaac gattattggt gtcgtaataa aacaatatcc gctcaaaaac
     6001 cgctttatgg cccatagcgg ttaaattaaa caggcctggg cgcagtttac tggggtgaaa
     6061 ataataaata tcaccgtcgc tagttttaag cgcgtagccg tcaccctgac caccaccatc
     6121 ccgactcagc caaaggcgtt catcgcggtt aaaactgact tcatcgccgg taagaattgg
     6181 aaatgccacg gggcggccgt cacttaagcg aatggcgagc gcaccatttt cttgcattaa
     6241 tgccaggtcg taattgtggt gccagccatg gccgaggggg ccttggtaaa tagaagtgct
     6301 aaaccaggtt cgctcccatt taaaggcaat ggggcccggc agagtaatat ccactgcatc
     6361 ggtaaataat ttcccggcgg ccacatccac cgggtggccg gttacggtac aaatggcttt
     6421 gtgcacttta ttgcggatat tcggcggcac gcctaattta tccataattt ttttggcgcg
     6481 ctcgtgcacg gctttggaaa gttttttaaa gcgggcactg cgtttttgta aggcgcgcaa
     6541 ttttttcatg gctttaccga gtgcggcaaa acccaccttc atggccatag ccatcatatc
     6601 aatggtgggt ggcccaccta ccaatacagg cataccggcg ggaataccca tcacaatact
     6661 ggagggcaat accatgccgt aggttttttt cggttttttc aggcgaatgg gcgaaatcat
     6721 gcctacgtct tggcaaccca aaacaggcag ggcggtaaaa cttaaggggg agccatcggc
     6781 taataccgtg gcactgccca taaaaatttc gtcttcattc atgggcggtt ttacaaatgg
     6841 cccgcccatg ggaatatggg gaattacttt gcctgaagtg ccggcagtac cgcggggaat
     6901 accgccgact aaaacagttg cgccgataat aggaatataa tccatcgggt ccaacaccag
     6961 ggctatatgc gggtgtggaa taggcacagg ccccaccggt gggatggcga caatatgaat
     7021 atcaatcccc atcaccggat caaaatgttt aatgattggc atcatgctgc agcgccctcc
     7081 agtttttcca accagtcgct gcttaataat tcactaaagg ttgcattaat atctttttgc
     7141 atttgggggt ttgactttaa tcgcaacata gcctgcccca caaaacccaa ggtggattgc
     7201 gcgcgaatct ctggggccat atttttacct acagcaaggg caaaattcgc ttgctcccaa
     7261 gcgcgctcag tttgatttaa acgctcggaa caaaacgccg ccatgcgcca ggcttccaag
     7321 gcgatccatt catcgtttaa taaagcagct tgctgagccg ctttttcata ggcaattacg
     7381 gcttggttta agtgttgggc gagaaaatta gctgtacctt caaataaaag cgcttgtaat
     7441 aataatttgt cacagccggg aacggcattt tctatcccca tgcgggcaaa taattgcgcc
     7501 tgttgatatt caataatagc ggcagggaat tgttggaagt ttaataagcc acctgcgcgg
     7561 gttaataaca cagtggcggt catatccggc cattgattgc tgcgggtaat ctctagggca
     7621 gcttcagctg cgctatttaa ttcagcgcta ttttgctcgg taatagcggc gttcatctgc
     7681 aaaaataata ggcgcaattg tgcgctatcg gtgccgtcat gggtttgctc ggccacagcc
     7741 cgggccgccg catttaaatt aatgggcggc gtttgggttt tggccacatc ggtaaagcct
     7801 gcaaatacag gcgttaaact aggctgggcg ccgttgtcta gggccaacaa ttttagtttg
     7861 ccaggccaag tggaataact gcgggcaata tggcaggcct gctgccacca caggcaatac
     7921 tcggttaaac ttttaatacc ggtgggattg ataaccaggg ttaattgctc aaggctttta
     7981 tcaaatagtg ctaatacgcg ctcgatggat acaaacaatg catgcaaacc actctgggcg
     8041 gtgtttagat ccggcgcctg ccaggcattt attaattctg gcgcttcggc agtttcccgc
     8101 gccgtttctt cctgggcttc ctcttcaagg gcttctgcta gcccctgttc aattaagcga
     8161 ttaaattctt cagccaatgt atagccatag cctgcggcag ttgcaaacgg gctagttagc
     8221 tgaataaacc aggtgcccga ttcttcgtcg aactgttctt ttatttttac caaggcattt
     8281 accaacagtg tatcggcagg attaatcagc cagtgcaaaa tagcctgctc gccgaggata
     8341 aattcatccc agtgctggtt gagtatcgtt acctcttgtt caatggcatt catagcaggc
     8401 aggccctaac gcaagtcttt tttaacattc taattaggta ttggctaatt taatttaata
     8461 atcggcccac tcaccattac tccggcaggg ttaatcgaga cggaacttgc ccccatggat
     8521 agagtaatac ccgctggagt aatcgtaata ctcgacggcc cgacgctaag cgatatttgt
     8581 gcagtgccgg attggctaat ttgcaaggct gacaattcag cattggtagt aatggatacc
     8641 gactgagcca gggcgctgat gtcttgtttg ccacccactt gaatagtttg attagcgcct
     8701 acagtggttt cttggtttac gccgatggaa accgtttgat tagtgccgac ggataactcc
     8761 tgattagcgc cgatactttg cgtgttgttg gagccgaccg tcaaagtgct attgccgtta
     8821 atgctgtcgt cactattgcc cacaatggtt tgcgttgaat tgccttgaat ggtgctgtct
     8881 tgattgcctt taacccctgc agtgcggtta ccggtgacat taaaactatc gttaccgcca
     8941 gtggaatgag aggtatcgcc cgccacagtg gtggaggcat tgccgtccac tttggtagtg
     9001 gtattaccca ctgtatggcg gctggcatcc ttgccgactt tcatgttgta atttttttgc
     9061 gcattaatgc gaaattcttc cgcatctttt ttatcgtcga ttaccaattc gttgtaggtg
     9121 ccgccatcgc ctcctttgct ggagcgggat ttcatcccca tcatgctttt attatcgggg
     9181 aggctatagg gcggtgtatt agcgctgtta tatacgctgc caataattaa gggttggtct
     9241 gggtcgccat taataaaagt caccacaact tcttggccta aacggggaat ggtaattgag
     9301 ccccatttac tgcccgccca ctgggtggca actcgcaccc agcaggagct ggtttcatct
     9361 tttttaccgt agcggtccca gtggaattgt acttttacgc ggccgtattg atcggtataa
     9421 atttcttcac cagcaggccc caccaccacc gccgtttgca cgccgtcaat gcgcggcctt
     9481 ggggtattaa aagctggccg aaaaggcgtt tgggcgggaa tacatacaaa ttgattggca
     9541 tagggcgggt cctgcgcttc actttctaaa aagctcgggt tgtacgccgt gtgctgaatt
     9601 tgggtaatca cataactttt attgtgatcg gcctgggctt cgtcggattt aaaggtgaat
     9661 tttttgccca cctcaaaact gtgaatatta ctggcgccat gggcccgctc gtggcctagc
     9721 tcttcctgtt gcatacgcaa tttagtcagc gctgtgcctt gatcgctctc ccgatagcta
     9781 cccggataag caaattgtgg gaaggaactg ttatttttta atttcaggtg ggtggcagtt
     9841 tgcgtgagca gagattgggt aaatttttca aaatcaaaat cggtttgcgc gaaggcgccg
     9901 gagcaatatt catagttatg ttgccactgg cgaatatatt catcccggcg aatacccgat
     9961 gaatgttcgg cgcttgtggg cacacaatcg ccataatggt ggggcgaatc ggataggtgc
    10021 agaatgtttt tatccggctg gtgctcaata aaataataaa tgccctcctg ctccagcagg
    10081 cggcaaataa agtccaaatc ggattcgtta tattgcacac agtattcaag aggcttatgc
    10141 acttccatca gtgcaaatac ataatcaaaa aagcccagct ccttaaaaat aaactccaca
    10201 atatccttca cacttttatt ctggaatacg cggcaatccg tgcgtttgct taaaaaccaa
    10261 aaccagggca ccacctcagc ccggtaggca tataggccgc gcttttccaa cccgcaatac
    10321 tgaaagcttt taacaaagcc gttgataaac cgtgcagccc cctctcccgg ttgaatacag
    10381 atactcacat tatcgcccac tatatttaaa gggttaatgg cgccatcgtg ggataataaa
    10441 tccaattcaa actgaaataa ttgggaaatt ccctcggttc cgcttaagcg gcgcaggagt
    10501 aaaacatcat tgcccaggcc cgtttgaatg cgcaggtggc gagtttcctg ggtaaataat
    10561 gcagcgggcg taaaacctag cgacataaca gctcctaaag cttaggatga gtgagtttaa
    10621 tgccagagat ttagagtgca gccatgggca gcggcacggt taattaaaat tgccttaacg
    10681 gcttagccgc gtacacgctg gcaggacgcc ttaaggctat tagtaccggg gatctttacg
    10741 ggggaatacc ttgcacacgc agatgtacaa agtggctata caaaaaacta tgcaaaacaa
    10801 aagcgactat gcaaaggcga cgacgcaacc gattattgtg cactgagcgc ctgcgcgccg
    10861 ggccagtata tctctatgcc ttagcccgtc aagggtacta ttaccaggcg ggttgcgcgg
    10921 tggcggcggg attaatatcg tgcgatgtaa ttcattcagc ggggtagata atcctaaaaa
    10981 ctgcagttga actctactac ggccgcctaa tacttgaatc tgtacgcttt tttgtcaaaa
    11041 tttgactcac cgtagtggtg actactgctt tcgccaaatt ttgacaaaaa agcgcccata
    11101 ttcagcgtct taggcaccct cgctacgagt tcaactgcgg tttttaggat aatgagcgcc
    11161 cagggcggct ttgacgttta tagaacccca ggcggtatgg ggcaataatt caccttaggg
    11221 ccgcgtgcgc gcactgccta aagggtatca accaggtatt gcaggcctac gccgtctttt
    11281 tccacccgca caatgcgcat atccaccacc ggtgcgtctt cgggcatatc ttgaatttgg
    11341 cccttcacaa tagaacccgg cggcggtaaa tccgtaggtt ttacgatgat aaataatccc
    11401 ccctcggaaa aatttctcgt caccaccacc ttctcgccaa aatccgggtg ggcaattttg
    11461 atgttcacgc gtaggggaat tcgcgggtgc tcgcgccgct cgtgcatggt gttaatccgc
    11521 taggagaaga tttataaact gtaggctgga aatagcagcc tagcaaccag cccccaaggc
    11581 gggccgggca attaaaccgg ctcgctctga aggcagggta tactcacatt tttactcgga
    11641 cactaaggct ccaccatgcc catccgcctg cttcgccctc tttgctgcct gttagttgta
    11701 tccttaagcg gctgtattaa gctgcaccta ctgccggaag actccatcca acacagcgtg
    11761 gacgcgggca aaaatctgat cgatcaggcc aaactcgccc gtgcaggcgg caaaaaaatg
    11821 accctcgcca gcgaagtaaa attatccgca gcggctactc aaaaggcggc ggaacaggcg
    11881 tgcctcgccg aattaaaaac ccgcgccgcc caggcctcca gcaaacgcgc ctatgaatta
    11941 gtgtcggaaa aaacccacct tgggaaaact gatggcatag aaataattgg ctgtgagatt
    12001 ttaatttatg tgtggaatta acacgccaag ccataatttc tcgccacttc aatgcccagc
    12061 caatgccctg ccgcctgctg tgtcagctta ttaacccggc ggctttttat gccgggttaa
    12121 taactgcggc aaggatgccg cgccaaaggc gcaagccgcc gcgagctccg ctaaaacaag
    12181 aaaatccacg cattttcgca agtgtcagcg gagcgagggc caacgatcta tttgaccgcc
    12241 gggttaataa gtggcccgcg ccataccttt tggttatggg tcactgaaaa acaaccattt
    12301 ccctcttgtg cgcacaaatc ggatgatggc cccacatcat ccacaccaca aaaggtaaac
    12361 accatgaaaa acatcccttc attaagcacc gtattagcct ccgccttcct tgcttccgca
    12421 agtttaagcg tcccagccca gagcgccgat ttcgccattc aacccctggc ccagggctac
    12481 caactggcgg agaacgcggc ggcaaaacct gccgaaggca aatgcggcgc cgccaccgag
    12541 ggtaaatccc acgagggtaa atgcggcgct gagcataaag atgatggcga gaaaaaacct
    12601 gaaggcaaag ccaaagaagg taaatgcggc gaaggtaagt gcggcgccaa caaaaagggc
    12661 taggccataa aacaggcggc ctagtgccgc ttttttttta gtaacccggc agctttttat
    12721 gccgggttac taatcctgcg ctcggcggtg taaaccgccg cgcgccgcgt taaaacaaga
    12781 aaatccacgc attttcgtaa gtttaacgca gcgacggcac aatagatcag gacgatctat
    12841 tgtgccgtca ggttaataat agcaaccagg aggctgttca ctttttatga gcactgacta
    12901 tgccaaacct aatgccgaat aaactcccca gcctaaaacc ccaagccctt tggcagggcg
    12961 caggtttggg cctgcacgca cgccacttgc ccgaattact cgccacccca cctgcggcgg
    13021 tagagtttat ggaaattgcg ccggagaatt ggctgaccaa aggcggccaa agcgcgcggc
    13081 aattacaggc gcttgccgag cgcatgccct tggtggcaca cggtttaaat ttgaatttag
    13141 gcggcaggcg cccactggat aaagaattaa ttctggccat aaaaggcctg caccacagct
    13201 taaatttgcg cttttattcc gaacatttaa gctggtgcgc cgatgagggc aatttttacg
    13261 atctcttgcc cctgcccttt accgccgaag ctgtcctgca tgtggcggcc cgcattcgcc
    13321 aggtgcagga tatgctggag tgttttatcg gggtggaaaa tatttcctat tatttacccc
    13381 aaaaccccgg cgacttatcc gagccggaat ttattaccgc cgtgctgcaa gaggccaact
    13441 gcctgctgca tttagatgtg aataatgtgt acgtcaattc cattaaccaa ggcatggatg
    13501 cgcgcgcgtt tatcgatgct atgcccagcg aaaaaattgc ctacatgcat attgccgggc
    13561 acagccaaga ggctgccgac ttaattatcg acacccatgc agcaccagtg gcggacccag
    13621 tgtgggattt attggcctac acctatgcca ccaaaggcgt ggtgcccacc ctactagagc
    13681 gggattttaa tctgccgcct ttggccgaat taaatcagga agttgcgcgc atcagttgtg
    13741 tacaacatca atacgcttaa gggcaaaaat ctatgcaacc gcctatttac ctgccttgcc
    13801 gcactagcgt tttatcctta gctgagtatc aagctgattt tgccgaaggc ctgcgatctg
    13861 actccagcaa gcgccagcag gcctataacg gccgccttgg cctgtataaa aaattaattt
    13921 ttgccaatct gtgcagtttt attgatagct gtttcccggt ctgccgctcc ctactaagcc
    13981 ccgccgtttg gcggcaatta tgccgcgcat ttttgcgcga gcacgcctgt caatcgcctt
    14041 tgttccatca gattccgctg gaatttatca gttggctgca aatgccacat cggtttcgcc
    14101 acaatttaga gcccgcttat ttaacccatt tggcccacta tgaatattgc gaactgcaaa
    14161 tacaaaccgc gccggatatt aatcactatg ctaaacccaa taattgcgcg ctatttataa
    14221 cacccatcca gttggtctgt tatccctacc cggtgcatac catagggcct aaaaatcctg
    14281 cgcccgcctt ggccactacg cggcttttgt tgtggcgcaa ggcagatgac agcgcaggtt
    14341 ttgcagaaat taccgccaat atttattgcc tgctggaatt actacaacac aaacaggatt
    14401 tagctgaaat ttttacccac tgggcacccc tatgtacacc ggctgctagc ctggatgaat
    14461 ttgttgccag tatgcaaaat ttagcaaccc tcggcgtgat agaaattcac ccctaatggc
    14521 ttatacaaaa acgacttaaa caaaaacggc ttaaacaaac caaactggcc tagcagcttg
    14581 ttgaaatacg taggcggcga cgcgctggca aggcaaattt ggcga
//
LOCUS       DAOQGS010000025        14498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1914_length_14498_cov_5.008309, whole genome shotgun sequence.
ACCESSION   DAOQGS010000025 DAOQGS010000000
VERSION     DAOQGS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_14498_cov_5.008309"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKZ52_01760"
     CDS             complement(<1..843)
                     /locus_tag="PKZ52_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HNG58519.1"
                     /translation="MTETLIKVDLSTSPYDNEGIHNRWHPDIPMAATVKPGDDFILEC
                     YDWTGGQIANNDNADDVRDVDLSQVHFLTGPVGVEGAEPGDLLVVDILDIGAFSNSLW
                     GFNGFFSKKNGGGFLTEHFPEAQKSIWDFNGMFTSSRHVPGVEFAGLIHPGLIGCLPS
                     KEMLAEWNAREKALFDTEPERTPPLAALPYADTAHMGKLKGDAAKAAAAEGARTVPPR
                     EHGGNCDIKDLSRGAKVYFPVYVKGGGLSVGDLHFSQGDGEITFCGAIEMAGWIHLRV
                     NLIKD"
     gene            complement(891..1580)
                     /gene="urtE"
                     /locus_tag="PKZ52_01765"
     CDS             complement(891..1580)
                     /gene="urtE"
                     /locus_tag="PKZ52_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNG58520.1"
                     /translation="MLSLSDYTVSYGQSEVIHGIDFSVGKNEIVAVVGRNGMGKTTLM
                     KSLVGMIAHKAGQIILDGLNVSKLKSYERIGAGLAFVPQGRMIFSTMTVKENIQTGLA
                     TGRNTTIPSYLYDMFPVLDEMKSRRGGNLSGGQQQQLAIARALATEPKVLVLDEPTEG
                     IQPNIIKDMGNTLKKIRDQKGISILVSEQVLSFALDIADRILVMEKGIIVKNQLRSEV
                     NEAQIASYLSV"
     gene            complement(1591..2337)
                     /gene="urtD"
                     /locus_tag="PKZ52_01770"
     CDS             complement(1591..2337)
                     /gene="urtD"
                     /locus_tag="PKZ52_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNG58521.1"
                     /translation="MFMSQDFLLSIEGLTVSFDGFKAVNDLSLYVDEGEIRVIIGPNG
                     AGKTTVLDLICGKTRASSGSIKFRGKELTRLREHAIVHAGVGRKFQNPSIYDDLTVFE
                     NLEISFPRGHSVFGALAFKRDVEVIQSVEETAGMIFLQDSLYKTAGLLSHGQKQWLEI
                     GMLLIQKPKLLMLDEPVAGMSIAERKQTAELLHRITKNHSVLVVEHDMQFVEDIADRV
                     TVMHQGKVLSEGSMEKVKNDPKVKEVYLGH"
     gene            complement(2343..3479)
                     /gene="urtC"
                     /locus_tag="PKZ52_01775"
     CDS             complement(2343..3479)
                     /gene="urtC"
                     /locus_tag="PKZ52_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNG58522.1"
                     /translation="MMLSSTKTFMPKNDFIGFLVLAAILLVVFPLFLDAFRLNMVGKY
                     LTYAFVAVGLVLCWGYGGILSLGQGVFFGIGGYCMAMFLKLESSDPESTKIQSTPGIP
                     DFMDWNQITALPAFWEPFHSLPFTLLAVLFLPALFAYIIGVAMFKRRVGGVYFAIITQ
                     AVASILTILIIGQQGYTGGVNGITDLRTFTGWDIRTDSAKNILYFVNAALLIICLLVA
                     QWVRTSKFGRLLVAMREREDRVRFSGYDVSSFKIFIFCLAATFSAIGGAMFTLQVGFM
                     SPSFVGIVPSIEMVIFCAVGGRLSILGAVYGTLLINFSKTAFSESFPELWLFAMGALF
                     IAVVMAFPNGLAGIYQTYVAPKLINLLAQLGKPKPAKSVINSAH"
     gene            complement(3602..4531)
                     /gene="urtB"
                     /locus_tag="PKZ52_01780"
     CDS             complement(3602..4531)
                     /gene="urtB"
                     /locus_tag="PKZ52_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNG58523.1"
                     /translation="MFGDYSTAELLSIFAMQGFAGLSLFSVFVLMALGLAIIFGQMGV
                     INMAHGEFMILGAYITYLVSSLFSNYLPSLFSAYFFVAMIVAFFAAGALGFLVERVMI
                     RHLYKRPLDTLLATWGLSLIMQQTYRSVFGAREVGVSLPEWMMGSLHPTDSIEIPING
                     IFVMVLTLFISLAVYYWMYRSRSGGQVRAVVQNRPMAAAVGINTQRVDAMTFALGCGI
                     AGVAGSAFTMIGSTGPTSGQLYIVDTFLVVVFGGASSLLGTIASAFTISQAQSTMEFF
                     MSGSMSKVLTLLAIVIILMLRPQGLFSLRMRHH"
     gene            complement(4600..5871)
                     /gene="urtA"
                     /locus_tag="PKZ52_01785"
     CDS             complement(4600..5871)
                     /gene="urtA"
                     /locus_tag="PKZ52_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNG58524.1"
                     /translation="MLSQNCQSSSNRSTFIAQKSSHYLKKLSVLFSSALLCGASIAGT
                     TTAEVNTTGLAVTDSTVKVGILHSVTGTMAISETGSVQAEKLAIEQINAAGGVLGRKI
                     EFIQEDGASDWPTFAEKSKKLLVNDKAAAVFGCWTSASRKAVLPVFEQYNGMLYYPTF
                     YEGLEQSPNVIYTGQEATQQILAGIDWVVKEKGAKTFYLLGSDYIWPRTSNKIARKHI
                     EKLGLSVVGEEYYPLGHTQFNSVINKIKLKKPDVIYAIVVGGSNVAFYKQLKAAGIEL
                     SKQTLLTISVTEDEIQGIGGENIAGAFAAMKYFQSLDNDNNKAFVKAFKEKWGANVVI
                     GDVTQAAYLGPWLWKAAVEKAGSFDIDKIKKASPDIELKTAPEGYVKIHPNHHLWSKT
                     RIGLAQPDGQYKVVYETKELMEPNPFPAGYQ"
     gene            6193..6372
                     /locus_tag="PKZ52_01790"
     CDS             6193..6372
                     /locus_tag="PKZ52_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58525.1"
                     /translation="MDAFIAVSIAVNGVVFCAIARLDEKTFSCCGGFGQQNASVFSAQ
                     FGLNRCALKTVIRLI"
     gene            6378..9731
                     /locus_tag="PKZ52_01795"
     CDS             6378..9731
                     /locus_tag="PKZ52_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG58526.1"
                     /translation="MNAPQQIFRVRRQYNQWVNNQTLEDYALRFTAKHARRWSPLSVA
                     TTALGGISFLALEAIGAVNTLSFGVANTLLATCYVALIIFIMGTPICYYAAKYGLDID
                     LLTRGAGFGYLGSTITSLIYASFTFIFFALEAAIMALALEHLFNLPLFIGYVVCSLIV
                     IPLVTFGITLISQLQKFTQPVWIILQLLPFIALFSTQGDTLGQWINYPGTTGRGEVAI
                     MYWGAAAAVLFALVAQIGEQVDYLRFMPEKTPANRWQWWTNLLLAGPGWILLGAAKIA
                     AGSLLAYLAIGQGLNPVDASDPTHMYRVGFSYLTPSPTLALVLAAIFVMVSQIKINVT
                     NAYAGSIAWSNFFSRLTHSHPGRVVWLIFNVFISLALMELGIYHAFEATLGIYALIAL
                     AWLGSLFADLTINKPLKLSPQHIEFKRAYLYSINPVGVGSMVLASSLGVASYLGGLGE
                     ELKAWAPFITLGSTLLLVPGIAWLTGGRYYLARHHELPMAATHHTCCICQNTYEPEDI
                     ALCPAYNGPICSLCCSLDSRCNDACKPKHNTTQVMLGWLQKYLRSTINPAVGRLSVHF
                     LALFILLGIVTAVLLHLVMFNVQPTSLETRELLQHTLLKVFFILMIIAGVIIWLVVLA
                     HESRQVAQDESQRQNRLLLEEISAHRATDLQLQKAKEQAEAANNAKSRYLTGISHELR
                     TPLNAILGYAQLLEHDPEIPAAKREKISVMRNSSQHLEDLIEGLLDISKIESGRLDIR
                     RNKVNIRELFEQVVSLFRLQAEEKNLAFIYQLTSPLPERVITDEQRLRQILINLLSNA
                     IKYTQEGYVKLQVKYRNQVAEFSVEDSGVGIDESDREIIFKPFERIRRPGVPSVTGTG
                     LGLTITRLLVDIMGGELEVSANKPRGTRFTVWLMLSSVVDAEPSHTQTPQQPMGFLGA
                     KKTVVVVDDDPAHRGLIGDILIPLGFNVVECADAESLLKLRSQLAPDIFLLDVNLPGM
                     QGWELVHHLRRYFPEVPLVMLSANVHEEQGADLIHPLHTAYLLKPIKVAQLLGVLQKS
                     LNLTWQYAPLVAQRSGGQQTPALLGLSRAQIEQAVEWAKSGYFSALKNTFNEWEAQLG
                     ADNAHIISLKTLLHAMQFNRIIEYLSDYL"
     gene            9728..10639
                     /locus_tag="PKZ52_01800"
     CDS             9728..10639
                     /locus_tag="PKZ52_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNG58527.1"
                     /translation="MKTPEFTGPCVLIVDDSADTLSMLNDTLEQAGMNCLLALDAKQA
                     LSIAGRMLPDIILVDAIMPNTTGFDLCQQLKYERELHDIPVIFMTGLNDAESLVKAFS
                     VGAADFITKPINTAELIARMRVHIANTRIRQSAQSALDQAGHYVFAVNKTGALLWSTP
                     LVNQLLQQAGADDEWMNSQLPQQLKTWLARNPMQGNALVISAPEREFSLILLGHSGPG
                     EILLRLMPNQGLDESQVLREHLQLTQREADVLLWISRGKTNREMAQILEISPRTINKH
                     LEQVFKKLQVDNRTSAARVALEIFAQK"
     gene            complement(11500..12192)
                     /gene="aqpZ"
                     /locus_tag="PKZ52_01805"
     CDS             complement(11500..12192)
                     /gene="aqpZ"
                     /locus_tag="PKZ52_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNG58528.1"
                     /translation="MQKYAAELIGTYWLVLGGCGSAVLSAAFPSVGIGLLGVSLAFGL
                     TVLTMAYAIGPISGCHLNPAVSIGLWAAGRFPATQLAPYIGAQLVGAIFAGATLYLIA
                     SGKAGFDISAGFASNGFAEHSPGGYSLQAALVCEVVMTMIFLVVILGATDKTAPSGFA
                     PIAIGLCLTLIHLISIPVTNTSVNPARSTGVAVFVGGWAISQLWVFWLAPIAGALLGA
                     WVYKLIGEKNNS"
     gene            13090..>14498
                     /locus_tag="PKZ52_01810"
     CDS             13090..>14498
                     /locus_tag="PKZ52_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNG58529.1"
                     /translation="MQQTPTSDQDALGDDRHALLQAYGTDLLDAVPAGIIVLNEAGII
                     VYCNSEVARLFAYDLSELQGQPVETLMPQAYRQQHVQLRQDYFTQSTPRFMGLGRDLF
                     GQRKDGSQFPVEIGLRPLMVGMQQFVVASILDISERKKAHEQLATVIDSSPYGKLVVD
                     KSGVIRLVNHRLVDLFGYSKEQLLGQAIEILLPERYRDAHGGLLQHYFAKPSLRAMGE
                     GRDLTGRHRSGREIPIEIGLAPLDFTGELHVLVCITDITQRKRLESDLREANAQLEEF
                     TFVVSHDLKSPMRGIADLTEWIIEDLGTAVAPSVANNLQRIQTRIARMENLTEDLLLY
                     ARAAKRSKEVAQVKLPDLIKGILELLVLPEQAQVHVHFAIPELITAKAPIETVLRNLI
                     SNAIKHHHAPESFQLSISAEPAGSYLCIRVQDNGPGIPQAARERVFRLFQTLQGSGTG
                     LGGVGLAVAKRLTEAHGGR"
BASE COUNT         3762 a         3510 c         3560 g         3666 t
ORIGIN      
        1 gtctttaatt aaattcaccc gcaggtgaat ccaaccggcc atttcgatgg cgccacaaaa
       61 agtaatttcg ccatcgccct ggctaaagtg taaatccccg acggataatc caccgccttt
      121 tacatacaca gggaaataca ccttagcacc gcgggatagg tctttaatat cgcagttgcc
      181 gccgtgttcg cgcggtggca cggtgcgggc gccctcggcg gcggcggctt tggcggcgtc
      241 gccctttaat ttgcccatgt gggctgtatc ggcatagggc agggcggcaa ggggcggcgt
      301 gcgctcaggt tcagtatcaa acaaagcctt ttcccgcgca ttccattcgg cgagcatttc
      361 tttagagggt aaacaaccga ttaaacccgg gtgaattaag cctgcaaatt ccacccccgg
      421 cacatggcgg gagctggtaa acatgccgtt aaaatcccag atggattttt gcgcttcagg
      481 gaaatgctcg gttaaaaagc caccgccatt tttcttggaa aaaaagccgt taaaacccca
      541 taggctattg gaaaatgcac ctatgtctaa aatatccacc accaataaat cgccgggctc
      601 agcgccttca acacccaccg ggccagtgag aaagtgcacc tggctgaggt ccacatcgcg
      661 cacgtcatcg gcgttgtcat tattggcaat ttgcccgccg gtccagtcgt agcattccaa
      721 aataaaatca tcgccgggtt ttaccgtggc ggccatggga atatccgggt gccagcggtt
      781 gtgaatgcct tcgttatcat agggtgaagt ggataaatca acttttatta gtgtttctgt
      841 cataacaaaa ccctctaggt tggcgggaac ttaatgtgta ggccgtgcat ttaaaccgaa
      901 agataagaag cgatttgtgc ttcgttcact tcgctgcgca attggttttt cacgatgatg
      961 cctttttcca tcaccaaaat acgatcggca atatccaagg caaaactcaa tacctgttcg
     1021 gataccaaaa tagaaatgcc tttttggtcg cggatttttt ttaaggtatt gcccatgtct
     1081 ttaatgatat tgggctgaat gccctcggtg ggttcatcca acaccaatac cttaggctca
     1141 gtagccagag cgcgggctat ggctaattgc tgctgctggc cgccggataa attgccgccc
     1201 cggcgagatt tcatttcatc cagcactggg aacatatcgt agaggtaact gggaatagtg
     1261 gtattgcgcc cggtggcaag gccggtctga atattttctt tcaccgtcat ggtggaaaaa
     1321 atcatccgcc cttggggcac aaacgcaagg ccagcgccta tgcgctcata actctttaat
     1381 ttagaaacat ttaaaccgtc taaaataatt tgcccggctt tgtgggcaat catgcccacc
     1441 agggatttca tcaaggtggt tttgcccatg ccattgcggc ccaccacggc gacaatttca
     1501 tttttgccca ctgaaaaatc aataccgtgg atcacttcgc tttggccata actgaccgtg
     1561 taatcgctaa gacttaacat acagttcccc ttagtggccc agataaactt ctttaacttt
     1621 cggatcgttt ttcacttttt ccatggagcc ctcagagagc accttaccct gatgcatcac
     1681 ggttacccgg tcggcaatgt cttccacaaa ttgcatatcg tgctccacca ctaaaaccga
     1741 gtgatttttg gtaatgcggt gcaaaagctc ggcggtttgt ttgcgctcgg cgatggacat
     1801 gcccgccacg ggttcatcca gcatcaaaag tttgggtttt tgaatgagca acatgccaat
     1861 ttccaaccac tgtttttggc cgtggcttaa taaaccggcg gttttataca ggctgtcctg
     1921 taaaaatatc atcccggcgg tttcttctac actttgaata acctctacat cgcgcttaaa
     1981 cgccagggca ccaaaaacgc tgtggccgcg cgggaaggaa atttccaaat tttcaaacac
     2041 cgttaaatca tcgtaaatag acgggttttg gaatttacgg cccacgcctg catgcactat
     2101 ggcgtgctcc ctaaggcggg taagctcctt gccgcgaaat ttaatggagc cggaggaggc
     2161 gcgggttttg ccgcaaatta aatctaacac ggtggttttg ccggcgccgt tggggcctat
     2221 aatcacgcga atttcgcctt catccacata gagggataaa tcattcacgg ctttaaaacc
     2281 gtcgaaggaa accgttaggc cttcgatact gaggagaaaa tcttggctca taaacaggtc
     2341 tcctagtgcg cactattaat gacggattta gcaggcttag gcttgcccaa ttgcgccaac
     2401 aaattaataa gcttgggcgc tacataggtt tggtaaatac cggctaaacc attgggaaag
     2461 gccataacta cggcaataaa taatgcaccc atggcgaaca accaaagctc agggaaggac
     2521 tctgaaaatg ccgttttaga aaaattaatt aataatgtgc catatacagc ccctaaaata
     2581 gaaagcctgc cacccacggc gcagaagatg accatctcaa tggagggcac tatgcccaca
     2641 aaggatggcg acataaaacc cacctgcagg gtaaacatgg cgccgcctat ggcagaaaag
     2701 gttgctgcca ggcagaaaat aaatatttta aaactggaca cgtcgtagcc ggaaaagcgc
     2761 acccgatctt cccgctcgcg catggccact aataaacggc cgaatttgct ggtgcgtacc
     2821 cactgggcaa ccaacaggca gataattaac agcgctgcgt tgacaaaata gagaatattt
     2881 ttggcgctat cggtgcgaat gtcccagccc gtaaaagtgc gcaaatcggt aatgccgtta
     2941 accccgccgg tataaccctg ctggccgata attaaaatgg tcaaaataga ggccaccgct
     3001 tgggtgataa tggcaaaata tacgcccccc acccgccgct taaacatggc aacaccaatg
     3061 atataagcaa ataaagcggg caaaaataat acggctaaga gtgtaaaggg taagctgtgg
     3121 aagggctccc aaaatgcggg caaagcggta atctggttcc agtccataaa gtctggaatg
     3181 cccggcgtgg actgaatttt agtgctctcc ggatcggagg attcgagttt taaaaacatc
     3241 gccatacaat agccgcctat gccaaaaaac accccctggc caaggcttaa aatgccgcca
     3301 taaccccaac ataaaaccaa gcccaccgct acaaacgcgt aggttaagta tttgcccacc
     3361 atgttgagcc gaaaggcgtc taaaaataat ggaaaaacaa ccaataaaat ggccgccaac
     3421 accaaaaagc caataaagtc atttttgggc ataaacgttt tagtactgga taacatcata
     3481 aaaccacccc gtgtacgcga atcatgcggc tcacctagtt gagctggata ttgccgcgat
     3541 cttttattaa tccggcataa aaagccgccg ggttaataag ttatgcaaaa aattaaacca
     3601 tttagtgatg gcgcatgcgc agggaaaata atccctgtgg gcgcaacatc agaatgatca
     3661 caatagccag aagggtaagc actttagaca tggacccact catgaaaaat tccatggtgg
     3721 actgggcttg tgaaatggta aatgccgatg caatggtgcc taataaactc gatgcgccgc
     3781 cgaataccac cactaaaaag gtatccacta tataaagctg gccagaggtt gggccggtgg
     3841 agccgatcat ggtaaatgcg ctgcctgcca cacccgcaat accgcagcct aatgcaaagg
     3901 tcatggcgtc tacccgctga gtattaatgc ccaccgctgc agccataggc cggttttgca
     3961 ccactgcgcg cacttgtccg ccggagcgag agcgatacat ccagtaataa acggccagag
     4021 aaataaacag cgtcagcacc atcacaaaaa tgccattaat aggaatttca atggaatcgg
     4081 taggatgcag agaacccatc atccactccg gcaggcttac gcccacttcg cgggcaccaa
     4141 aaacagagcg ataggtttgc tgcataatta aacttaaacc ccaggtcgca agcagcgtat
     4201 ctaacggccg cttatataaa tgccggatca tcacccgctc gactaaaaat cccaaggcac
     4261 ctgcggcaaa aaaggctacg atcatcgcca caaaaaagta ggcggaaaat aaactcggaa
     4321 gataattaga aaataggctg gacaccaaat aggtaatgta ggcgcccaaa atcataaact
     4381 cgccgtgggc catattaatt acacccatct ggccaaatat aatggcaagg cccaaggcca
     4441 taagcacaaa cactgaaaaa agacttaagc ctgcaaaccc ttgcatagca aaaatagaca
     4501 ataactctgc tgttgaataa tcaccgaaca tacaccaccc cactgattgt gattaaaact
     4561 ggaaaatcgg gtgcagactc tgtctgcacc cgcaatctgt tattgataac cagctgggaa
     4621 ggggtttggc tccatgagtt ctttggtttc atacaccact ttgtattggc catcgggttg
     4681 ggcaaggccg atacgggttt tggaccagag gtggtggttg gggtgaattt ttacataacc
     4741 ttctggggcg gttttcagct caatatccgg ggaggctttt ttgattttat ctatgtcgaa
     4801 ggaaccggct ttttcaaccg cagctttcca cagccatggg cctaagtacg ccgcttgggt
     4861 tacatcgccg atcaccacgt tcgcgcccca tttttcttta aaggctttta caaacgcttt
     4921 gttattgtca ttatccaggc tttggaagta tttcatggcc gcaaaggcgc ctgcgatatt
     4981 ctcgccacca atgccttgga tttcatcttc ggttacggaa atagtcaaca gagtttgttt
     5041 gctcagctcg ataccggctg cttttaattg tttgtaaaag gccacgttgg agccgcccac
     5101 cacaatggcg taaatcacat cgggcttttt gagcttaatt ttgttgatca ccgagttaaa
     5161 ttgagtgtgg cccaaaggat aatactcttc acccaccaca cttaaaccga gtttttcaat
     5221 gtgtttgcga gcaattttgt tggaggtgcg cggccaaata taatccgagc ccaatagata
     5281 aaaggttttc gcaccttttt cttttaccac ccaatcaatc ccggcaagaa tttgttgggt
     5341 ggcctcttgg ccggtgtata tcacattggg ggactgctct aaaccttcat aaaatgttgg
     5401 gtaatacaac atgccattgt attgttcaaa caccggcaat accgccttgc gcgaagctga
     5461 tgtccagcag ccaaatacgg cggcggcttt gtcattcacc agcaattttt tggatttttc
     5521 agcgaaggtg ggccaatcgc tggcgccatc ctcttggata aattctattt tgcggcccaa
     5581 cacgccacct gcggcgttga tttgttcgat ggctaatttc tccgcctgta cagagccggt
     5641 ttcactgatg gccatggtgc cggtgacgga gtgcaaaata cccactttaa ccgtgctgtc
     5701 ggtaaccgca aggcccgtgg tgttgacttc cgcagtggtg gtgcctgcga tacttgcccc
     5761 acagagtagg gcgctgctga ataatacgga taattttttc agatagtggg aagatttttg
     5821 cgcgataaaa gtggagcggt tacttgatga ttgacaattt tgactgagca taattattcc
     5881 tcaaaagacc agcgggaaaa tcgaggtgtc atagttgatc aaaatactca ggcaaaccat
     5941 gcggcaaatg tcgcaggcac ctacgtcatt taccccaggt gcgtctaggg ctacttggag
     6001 tggtttttga cacaccgatt aacccggcac taaatgccgc ctggttaata agtagtttta
     6061 gcgagatggg cgaaatgacg cctggcaatt ttaaaaactc cctgcttata caagggttgg
     6121 tgagggcgta tttcaagaag cgattaatgg cgccccagct tcattggcac ggcactggca
     6181 gtggcagaat ctatggatgc ctttatagca gtctcaatag cggtgaatgg ggtggttttc
     6241 tgcgctattg caaggctgga tgagaagacc ttttcttgct gtggtggttt cggccaacaa
     6301 aacgccagcg tttttagcgc tcaatttggt ttaaaccgtt gcgcactaaa aacagtcatc
     6361 agactgattt aacttctatg aatgcaccac aacagatctt tcgcgtgcgc aggcaataca
     6421 accagtgggt gaataatcaa accctggaag actacgccct gcgttttacc gcaaaacatg
     6481 cccgccgctg gtccccctta agtgtggcca ccacggcctt aggcggaata tcgtttttag
     6541 cccttgaggc cataggtgct gttaatactt taagttttgg tgttgccaat accctgctgg
     6601 ccacctgtta tgtggcgcta attattttta ttatgggcac acccatttgt tattacgccg
     6661 ctaaatacgg cctggacata gacctattaa ccaggggggc aggttttggt tatttaggct
     6721 ctaccatcac ctcattaatt tacgcctcgt ttacctttat attttttgct ttagaggccg
     6781 ccatcatggc cttggccctt gagcatttat ttaatctgcc cttatttatt ggttatgtgg
     6841 tctgctctct tatcgttatt ccgctggtta cctttggcat aaccctgatc agtcaattgc
     6901 aaaaatttac ccagccggtg tggattattc tgcaattgct gccctttatt gccctgtttt
     6961 ccacccaggg cgacaccctc ggccagtgga tcaattaccc gggcactacg gggcgcggcg
     7021 aggtggcgat tatgtattgg ggcgctgccg ccgcggtttt atttgccctg gtggcgcaaa
     7081 ttggcgagca ggtggactac ctgcgtttta tgcccgaaaa aactccggcc aatcgctggc
     7141 aatggtggac taatttactt ttagctggcc ccggttggat tttattaggt gccgctaaaa
     7201 ttgcggccgg ttccctcctg gcctatttgg ctatcggaca ggggttaaat ccagtagatg
     7261 ccagcgatcc tacgcatatg tatcgggtgg gcttttctta tttaacgcca tcgcccacct
     7321 tggcactggt gttggcggcg atttttgtca tggtgtcgca aataaaaatt aacgtcacca
     7381 atgcttatgc aggctccatc gcctggtcga attttttctc ccgtttaact cacagccacc
     7441 cgggccgagt ggtgtggctt atttttaatg tatttatttc ccttgcctta atggagctgg
     7501 gcatttacca cgcttttgag gcaactttag gaatttatgc gctgatagct ttagcctggc
     7561 tcggcagttt atttgccgat ttaaccatta ataaaccact taaactcagc ccccaacata
     7621 tcgaattcaa acgcgcctat ttatattcca ttaacccggt gggcgttggc agtatggtgc
     7681 tggcgagcag cctgggcgtg gccagttatt taggtggttt aggtgaggaa ttaaaagcct
     7741 gggcgccttt tattaccctt ggcagcacat tgttattggt gcctggcatc gcttggttaa
     7801 ccggcggccg ctattatttg gcgcggcacc atgagctgcc aatggctgcc actcatcaca
     7861 cctgttgcat ttgtcaaaat acctatgagc ctgaagacat agcactctgc cccgcctata
     7921 acggccccat ttgttcattg tgttgttcgc tggattcgcg ctgcaatgat gcgtgcaaac
     7981 ccaaacacaa caccacccag gttatgttgg gttggttgca aaaatatctg cgcagcacca
     8041 tcaacccggc tgtaggtagg ctgagcgtac attttttagc gctgtttatt ttgttgggta
     8101 tagtgacggc cgtattgctg catttggtga tgtttaatgt ccaacccacc tcgctagaaa
     8161 cccgcgaatt attacagcat accctgctga aagttttttt tattttaatg attattgccg
     8221 gcgtgattat ttggctggtg gtgctggccc atgaaagcag gcaagtggcc caggatgaat
     8281 cccagcggca aaaccggtta ttgctggaag aaatcagcgc ccaccgcgcc acagatttac
     8341 aattacaaaa agccaaggag caagcggagg cggcaaacaa cgccaaaagc cgttatttaa
     8401 ccggcatcag ccatgagctg cgcacaccgc tcaacgccat attaggttat gcccagctgt
     8461 tggagcacga cccggaaata cccgccgcca aacgggaaaa aatcagcgtt atgcgcaaca
     8521 gcagccaaca cctagaggat ttaatcgaag ggctgttgga tatttccaaa attgaatccg
     8581 ggcgtttgga tattcgccgc aataaggtca atattcgcga gttatttgaa caggtagtgt
     8641 cgttatttcg cctgcaggcg gaagagaaaa acctggcttt tatctaccaa ctcaccagcc
     8701 cattgcccga gcgggtaatt accgatgagc agcggctgcg gcagatttta attaatttat
     8761 tgtccaacgc cattaaatac acccaagagg gctatgtgaa gttgcaggta aaataccgca
     8821 accaggtggc ggaatttagc gtagaagact cgggcgtggg catagatgag agcgaccggg
     8881 aaattatatt taaacctttt gagcgcatcc gcaggccggg ggtgccgagt gttaccggta
     8941 ctggcctggg gcttactatc acgcgcctgt tggtggatat catgggtgga gagctagaag
     9001 tttctgccaa taaaccgcgc ggcacccgtt ttactgtttg gttaatgctc tcctctgtgg
     9061 tggatgcaga acccagtcat acacaaacac cgcaacaacc catgggtttt ttgggcgcta
     9121 aaaaaaccgt ggtggtggtg gatgatgacc ccgctcaccg cggtttaatt ggcgatattt
     9181 taattcccct cgggtttaat gtggtggagt gcgcggacgc cgaaagcttg ctaaaactgc
     9241 gcagccaatt ggcgccggat atatttttat tggatgtaaa tttgcccggt atgcagggct
     9301 gggaattggt gcaccacctg cggcgttatt tccccgaagt gccactggtg atgttatcgg
     9361 ccaatgtaca tgaagagcag ggtgcggatt taatccaccc cctgcacaca gcctatctat
     9421 taaagccgat caaagtggcg cagttattgg gcgtgctgca aaaatcgctc aatctcacct
     9481 ggcaatatgc gcccttagtg gcccagcgca gcggcggcca gcaaacgcct gcgctactgg
     9541 gcttaagtcg ggcacaaata gagcaggcgg ttgaatgggc gaaaagcggt tattttagcg
     9601 ccctaaaaaa tacttttaat gaatgggaag cgcaactagg agcggataat gcccatatca
     9661 tcagcttaaa aacactgctg catgccatgc agtttaatcg cattattgaa tacctaagtg
     9721 attatttatg aaaaccccag agttcaccgg cccctgtgta ttaatagtcg atgattccgc
     9781 cgataccctt tccatgttga atgacacctt agaacaagca ggcatgaatt gtttattggc
     9841 gctggatgcg aaacaagcgc ttagcattgc tggccgcatg ttgcctgata ttattttggt
     9901 ggatgcgatt atgcccaaca ccaccggctt tgatttgtgc cagcaattaa aatacgaacg
     9961 ggagttacac gatataccag taatttttat gacagggctt aatgatgcag aaagtttggt
    10021 aaaggctttt tccgtcggcg cagccgattt tattaccaaa ccgattaata cggctgagtt
    10081 aatcgcccgt atgcgtgtgc acattgccaa tacccgcatt cgccagagcg cacaatcggc
    10141 gttggatcag gcgggccact atgtttttgc ggtgaataaa accggtgcgc tgttgtggag
    10201 tacccctttg gttaatcagc tattgcaaca ggcgggcgcc gatgacgagt ggatgaatag
    10261 ccaattgccg caacaattaa aaacctggct cgcccgcaac cccatgcagg gtaatgcctt
    10321 ggtaatcagc gcgccagagc gggaatttag tttgattttg ctcggccaca gtggcccagg
    10381 ggaaatatta ttgcgcctaa tgcccaacca agggctggat gaatcccaag tgctgcgaga
    10441 gcatttgcag ttaacgcaac gggaggcaga tgtgttgttg tggattagcc gtggcaaaac
    10501 caatcgagag atggcgcaaa ttctagaaat tagcccgcgc acgattaata aacacctgga
    10561 acaggtattt aaaaagttac aagtagacaa tcgcaccagc gcagctcggg tagccctgga
    10621 gatttttgcg caaaaataag gcttgtgtgg ggaatagcga aaaccggttg tacctaaacc
    10681 attcctttac tttaggctaa atcaaatcag tccctgggat ttagccgatt ttcgctatta
    10741 gtactggcat gaacactatg caccatcgca atccttaaat tatgggtggg tagatcctgt
    10801 acaggggtcc gttggtatcc ttcttaataa tcccggcggc tccttattaa cccggtggca
    10861 aaataagtcg ccctgatcta ttttgccttc gcttcgctaa aacttgcgaa aatgcgtgga
    10921 ttttcttgtt ttagctgagc ccgcagcagc ttgcgccgca ggtttggcat ccttgccgcc
    10981 atcatttacc aggcataaaa agccgccagg ttaagaataa tccctaaatt ttcttttttg
    11041 tccgcccgcc atcatattag ctggcggtaa accttggtgt tagattaggg ctcggcgctg
    11101 gtggggtctg tgcgaaaaat tgctctgtgc cggtgaatta ttcacctggc aggccgttga
    11161 aatacttgca gcttgttgaa atacgtaggc gccgacgcgc tgacaaggca aatatggcga
    11221 aaaaaccgga cgactgcatg gatgcaatgc aggagcaatt gccgacgact gcatggatgc
    11281 aggaggtaga gcaatgcagg agcaattgcc gagtttacac gcagtaaatg aggatttttt
    11341 cgccaaattt aacgccgcca acgcttgcaa ggtagtattt aaacaagctg ctaggccagg
    11401 acggggtgtt aatttaatgg tcttggagta aggggcgggc taataccgcc cctggtgggg
    11461 tgcgcgctgt aggtatgtat tttagaaccc ggcgaataat caggaattat ttttctcgcc
    11521 aattaattta tacacccagg cacctagcag tgcgcctgca atcggtgcaa gccagaacac
    11581 ccataattga ctgatagccc agccgccaac aaaaacggca acccctgtgg agcgcgccgg
    11641 atttaccgag gtgttggtca ctgggatgct aattaaatga attagggtta aacacaggcc
    11701 gatggcaatt ggggcaaaac cgctaggtgc tgttttatcg gtggcgccga gaataaccac
    11761 taaaaaaatc atcgtcatga ccacttcaca gactagggcg gcctgcaaac tgtatccgcc
    11821 gggggagtgc tcggcaaacc cattggaggc gaagcctgcg gagatatcaa aaccggcttt
    11881 gccgctggca attaaataca gggttgcacc ggcaaatata gcaccgacta actgcgcacc
    11941 tatataaggc gctaattgtg tggcgggaaa gcgccccgct gcccacaagc ctatggacac
    12001 ggccgggttt aaatggcagc cggaaattgg ccctatggcg taggccatgg ttaatactgt
    12061 gagaccaaaa gccagggata cacccaataa gccgatgccc actgagggaa atgccgcaga
    12121 caacaccgcg ctgccacagc cacctaacac caaccaatag gtaccgatta attctgctgc
    12181 gtatttttgc atggttaaaa cctcaaaggt gaagggctaa aaacaactat gaagatagag
    12241 gccgctggct gccaccaaaa tgttttgctt ggcaaacaag atgcgtcgct tgccaaaata
    12301 cccatgagcc tgaatttgat cataggcttc taattaatgc tgatgtgaat tgaaagctta
    12361 tcgagcagca attattcggc caaataacaa gcagcttttt catggttatt aacctgggat
    12421 aacagctaac tcgacataga atctaccggt ttttagctgc ttcactaaat gtcggtatac
    12481 tccttaattg tgacagaatg gcagggatac ttttttgttt gcccagattt tgctttcttc
    12541 ttcataggcc cctaggctca gtattaaagg gggttcgcag tcaacctata actattaatt
    12601 taacgcgcca tgagtgttta ggctttttaa acccgcggtt ttgcttgaag cgttattgaa
    12661 cggtgctata cctagagacg agcagaaggc cagcagcggt attagttagc ccgataaatt
    12721 tctgtgattc atataaccct agctgttgga aggctaattt aatggccttt aaggcctggg
    12781 gatgccttgc aggcgggcga ttttttggta aataatggcg catctaaaat ttaaagccct
    12841 atgcgccatg ggtgctttgg gggtatctta gcgcccctgg ctcgggctaa aacaacagcc
    12901 tcagttaaaa caatagctac tgctaaacag tagccacagc gataacaaaa acaataaacg
    12961 ggcccatgca tagccgtatt acacgcgcct cgcatggcgg ctaggtaaaa agcccatatt
    13021 taatcttaaa cccctgttgt atttgggaat aatgtacgcg agcactaagc caatctcggg
    13081 gaattctaaa tgcagcagac acccactagt gaccaggacg ccctgggcga tgaccggcac
    13141 gccctgctac aggcctatgg cacagactta ttggatgcgg tgcctgcagg cataatcgtg
    13201 cttaatgagg cgggtattat tgtttattgc aatagtgagg ttgcacgttt atttgcttat
    13261 gacttgagtg aattgcaagg ccagccggtt gaaaccctaa tgccgcaggc ctatcggcag
    13321 cagcatgtac agttgcggca ggattatttt acccagtcaa cgccgcgttt tatgggcctg
    13381 gggcgcgatc tttttgggca gcgcaaagac ggcagccagt tccccgttga aattggtttg
    13441 cgcccgttaa tggttggaat gcaacagttt gttgtggcat ccattttaga tattagcgag
    13501 cgcaaaaaag cccatgaaca attggccacc gttatagatt cctcccccta cggcaaattg
    13561 gtggtggata aatccggcgt tattcgtttg gtcaatcacc gtttggtaga cctatttggt
    13621 tattccaagg agcaactgct cggccaagcc atcgagattt tattgcccga gcgctacaga
    13681 gatgcccacg ggggcttgtt gcaacattat tttgccaagc cgagcctgcg cgccatgggt
    13741 gaggggcgcg atttaaccgg gcggcatagg tcgggccggg aaattcctat tgaaattggc
    13801 ctggcgcctc tggattttac cggggaatta cacgtcttgg tgtgtattac ggacattacc
    13861 caacgcaagc gcttggaatc agatttgcgc gaggccaacg ctcagttgga agaatttacc
    13921 ttcgtggtat cccacgattt aaaatccccc atgcgcggga ttgccgattt aacggaatgg
    13981 attattgaag acttaggcac cgccgttgcg ccctctgtgg cgaataattt acagcgcatt
    14041 caaacgcgta ttgcgcgcat ggaaaacctc accgaggatt tattgctcta cgcccgcgcc
    14101 gctaagcgct ccaaagaggt ggcacaggtt aagctacccg atctgatcaa gggtattttg
    14161 gaattattag ttttgcctga gcaggcgcag gtgcatgtgc attttgctat ccctgagctg
    14221 ataaccgcca aagcccccat agaaactgta ttgcgtaatt taattagcaa tgccattaaa
    14281 catcaccacg cgccggagtc attccagctg agcatcagcg cggagcctgc cggcagttat
    14341 ttatgtattc gtgtgcagga taacggccca ggtattccgc aggcggcacg ggagcgggtg
    14401 tttcgtttat ttcaaacttt acaaggctct ggtaccggtt tgggcggcgt gggcttagcc
    14461 gttgccaagc gcttaaccga ggcccacggc gggcgctt
//
LOCUS       DAOQGS010000026        14443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1922_length_14443_cov_5.518210, whole genome shotgun sequence.
ACCESSION   DAOQGS010000026 DAOQGS010000000
VERSION     DAOQGS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_14443_cov_5.518210"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKZ52_01815"
     CDS             <1..680
                     /locus_tag="PKZ52_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNG58530.1"
                     /translation="GKDQLNSPLSIGAIFTIGPYLFPHFIPQLQQIAPQMPLYIEESF
                     TATLRRRIRQGELDAIVVALPFTEPDVVTQPLYEERFVVLMAANHPLAQQEAIAPDAL
                     TDYNLLLLGEGHCFRDQVIQACPNLKDHIEDPSGRVRTASEGSSLETLKHMVASGLGI
                     TILPSTAASGPHLAEGLLVAKPFTDPAPTRTVALAWRASFPRLKAIDALRQSIARCHL
                     GAVSRGA"
     gene            706..2787
                     /gene="recG"
                     /locus_tag="PKZ52_01820"
     CDS             706..2787
                     /gene="recG"
                     /locus_tag="PKZ52_01820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNG58531.1"
                     /translation="MPSARGSGAIAVRGLKGVGDAMATRLAKLGIYSLQDLWFHLPLR
                     YLDRTQITPMGELSLNSSVVIQGWVHSAQVQQGRRRSLVVVIEDDSGQAVLRFYHFTA
                     QQVQRFSPGTPLRCYGDPRLGVSGLEFYHPELEWLADAEELPDLAQTLTPIYPLTEGI
                     SQNRMRQIMAAGLALLEQYPPEELLAAEINQRFGVANLAQALRFVHCPPIEAQVPALL
                     AGTHPYQQRLAFEELLAHNLAIWQSRLQAKHEQAPVIAPPGMLARQLLHGLPFALTQA
                     QERVSDEIRRDMAQSAPMLRMVQGDVGSGKTLVAAMACAQAVEAGYQVVIVAPTEILA
                     EQHKANFARWFEPLGVSVGWLAGKLSAGQKRSVSEQIGEGALQIVVGTHALFEEHVLF
                     KRLGLVIIDEQHRFGVAQRLSLRGKNPDGAFPHQLVMTATPIPRTLAMTQYSDLDLSI
                     IDALPPGRTPINTVLINQTKRPAVIERVRAQCEAGRQAYWVCTLVEESETLAASAAEA
                     TASQLQAALPQLRIGLVHGRLKSRDKEAVMAAFKAGELHLLVATTVIEVGVDVPNASL
                     MVIENPERLGLAQLHQLRGRVGRGSVASHCVLLYADPLSRNGRERLEVMRASSDGFVI
                     AEKDLALRGPGELLGTRQTGALQFRIAQLQRDSGLLEWVQACVAEVAGTDVEQRLIQR
                     WFARQQGFVNA"
     gene            complement(3373..3921)
                     /locus_tag="PKZ52_01825"
     CDS             complement(3373..3921)
                     /locus_tag="PKZ52_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNG58532.1"
                     /translation="MSVRSFKGVQPTVGERVFVDQAAVVIGDVVLGDDVSVWPCAVIR
                     GDMHRIRVGARTSVQDNAVLHITHASKFNPDGWPLTIGEDVTIGHGACLHGCTLGNRI
                     LVGIGATVLDGAVVEDEVIIAAGALVPPGKRLESGFLYVGNPCKQGRPLKDSERAFFT
                     YSAQNYCKLKDEYLREENGAAS"
     gene            4046..6094
                     /locus_tag="PKZ52_01830"
     CDS             4046..6094
                     /locus_tag="PKZ52_01830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNG58533.1"
                     /translation="MTNPLLVPQGLPAFAQIRPEHINPAIDSIINANKQHLATLLNEL
                     SAPSWATLAAPLEEAEDRLDKAFSPVSHLNSVANTPQLRDAYNSALQSLTAYHTALGQ
                     NNELYQAYQALADSNEFATLSQAQQQAINLALRDFRLSGVSLAAEKKTRFAEIKARLS
                     QLTSLFANQVLDATQNFFHTITDEAELAGLPPLLIQQAKQLAEQKNSPGWVFSLDIPV
                     YLQIMTQAENANLRQLFYTAYMTRASDCGPQAGQWNNQPVMEEILQLRLEMAQLLGFK
                     HYADYSLASKMASSPEQVLEFLYQLAQQSRPMAEQELAELTQWVQATYGKTDLAVWDV
                     PYYSERLKEARYSISQEFLREYFPLPRVLNGLFSTIEQLFKVSIAPMHSEDLWHSDAA
                     LYGLYRDGELIAQCYLDLYAREGKKGGAWMGQCQVRRQTQAGLQLPVAFLVCNFSAPV
                     GDTPSLLTHQEVTTLFHEFGHGLHHMLSKIDVAAVSGINGVAWDAVELPSQFLENFCW
                     QKSVLKDLSSHYKTGEPLGDEWLDKMLAAKNFQSALQMLRQLEFAIFDMRLHMEYGSS
                     SFTSVQNLLDEVRKQVAVIIPPEFNRFQLSFSHIFAGGYAAGYYSYKWAEVLSSDAFA
                     AFEENGLFDSATGEKFLQCILERGGAVEAMQCFKDFRGREPSIEALLRHSGIHHDSTH
                     "
     gene            6078..6311
                     /locus_tag="PKZ52_01835"
     CDS             6078..6311
                     /locus_tag="PKZ52_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HNG58534.1"
                     /translation="MTAPIKKRFIAGAVCPKCASMDTVFTYRFDDKDWRGCVNCDFNE
                     AMMFTPQVRELETRVNLDEATKAAQTQVIKILK"
     gene            6506..7867
                     /locus_tag="PKZ52_01840"
     CDS             6506..7867
                     /locus_tag="PKZ52_01840"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNG58535.1"
                     /translation="MNKNKLTLVLSLIFASQGVLANNTTFDEFSPLIGSVPAGSLPET
                     APFKLANPAFTQQAFVINNSPTNYGDNWDMIDTNRTGVDAGRYLFTPYETGSAGVMRV
                     DTWTNTAQTIVAPGTQGFVSGDASRWTPWGSYLTAEESWGTGSTKGRLFEVTNPLAAV
                     GSINFVNRTTSLPNISHEGLAFDNANNLYFIDEVDGGAVYKFTSTKPNASNGNDFFAA
                     GQTFALKADAGKTGAFSWAALTDVNGGLLPATASADLASSSFDGRKAADLVGATNYNR
                     PEDMDIQHGVNGEILYVATTTDHEVYAINLSTNSVSLFASRNTINLATGVAVGTAFTN
                     PDNIAIDADGNIYVVEDQPGGSADIWMGKDLNKDGDLTDAGEGLARWLALSTVGSEPT
                     GLYFDVFNPTVAYVNVQHADSDKDTMIKLTSPAPVPLPAAVWLLGSGMLGLMGVARRR
                     SKA"
     gene            8977..10470
                     /locus_tag="PKZ52_01845"
     CDS             8977..10470
                     /locus_tag="PKZ52_01845"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNG58536.1"
                     /translation="MSFISVESRVSGKPNRSNHTSEFSAGEFSVVILGDFSGRASRAV
                     SLPLSRLVPVDRDNFFDLFSAFDIKLKLPFDEQPIEFKDFEHLHPDYLLDQLAIFAKF
                     RQWQRKALKPEHFESLLEELRAAKVWDDQAASVQTDLQANAHTSAEKADMSADFLLEQ
                     VLNQGVEGSAQTDVEQLIRSIVAPYATLKADIRLSEVQNAINQAMAQTLRSILHHGHF
                     HNLEATWRSLYFFVRRIASDNGIKIWLLDISEAELAQASQEEPTQSALYDLLVKQRQA
                     AGQSAHNLIINLFAADSNLTSLNWLKTLAAIAAANNGACVGAANPNIAGCSNLIGMPD
                     PSQWQGPPEDFATAWQSFRASADAAHLALTAPRFLLRTPYGKKSSPIETFDFTEMITE
                     GNHQHYLWGNSGVLVGVVLAELWLNGKGVINSAAASQIDQLPIHVEKTAAGEQLIPSA
                     EIVILDSTVAALRAAGLLVVRSIYNKNAVLIPLLNSCAADGALLYSR"
     gene            complement(10689..13595)
                     /locus_tag="PKZ52_01850"
     CDS             complement(10689..13595)
                     /locus_tag="PKZ52_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG58537.1"
                     /translation="MSIKLNTLKFTPKILAQCVVCVMLAQAARADVSDAGIQGEVRTA
                     RAAGQIIEATNVDTGRVITTRTQADGHFILNGLAPGNYSLKLQGSDASQLLRLRVGQI
                     TQVVFDEKNTQTEEVVVTGIVEKASKGGAIGAQISTEQIKNLPQTNRNFLSAADLAPG
                     VAFVQEDDGTSSMRAGASTPSSINVFIDGVGQKDYVLQGGVSGQDSSRGDPFPQSAIK
                     EYTVITQNYSAEFDQISSAAVVAVTQSGGNELKGGVFADYTTDHWRAQKPREKLDRYK
                     TPSNQYQFGANISGPIIQDAVHFFITYEGKNYADPKEVVPGDGADFTKLPASLGAKIG
                     GVVADFKEDLVFGKLDFTVNEQHALELTAKYRQETELAGVGGTNTLEHATDRDNRDVR
                     MALGHKWRGDFFLNEFKITHEDTQFNPNPHSNGYGQIYEREEQKELLKVGGGEDFQDK
                     GQKGLGLQEDFTYSGFVGHNIKTGLKYKAVTLHATEQQPYNPQFYYYQGAVSQPNRVR
                     FGAPLKDIGTGSVEADNTQWGIYLQDDWRATDALALNLGLRWDYEESDVYLDYVTPAK
                     VVSGINSWDAIKKSDVPINDYIANGHNRSAFKDAIQPRVGFTYALNDEGLELFGGAGR
                     AYDRNLFDYLQLETTKATYPTYEYRFIAPGVPGACATLSANCIAWDNKYYATDNLYAL
                     ANSDGAGRELYLLPNELKVPYSDQFSLGVRSPVGDWNLQASIAHITTQDGFAWLLANR
                     RADGNFFNPGKTWGTPWGNGIPGYGNTLIGASGLETRNQSLYLQAEKPRAESNWSFSA
                     AYTYTDAEENRKFREYFALDYPKFSDYSFLPATSVPEHKLVMAGSYFLPRGFTLSAKW
                     KLESQKPITGIDCRAGWEDCKYASATPDTGELFGFNQFDISLKKTIIDDPRYFGSGVV
                     LRLDILNLANTRNDKGFNDWFGGAGEQKPDNFGTSDNTIAGPMRTMKIGLSVDW"
     gene            complement(14213..>14443)
                     /locus_tag="PKZ52_01855"
     CDS             complement(14213..>14443)
                     /locus_tag="PKZ52_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58538.1"
                     /translation="ALTLAKMRGFSCFSAARGESHLYRGIHADIINPALNAAGLIIIF
                     SKAVTLIRQRAKPQYLPAFITCAQQPVKISCH"
BASE COUNT         3427 a         3887 c         3701 g         3428 t
ORIGIN      
        1 ccggcaaaga ccagctcaat agccccttgt ccatcggggc gatttttacc atcggcccct
       61 atttattccc gcactttatt ccccagctgc agcaaattgc tccgcagatg cccctgtata
      121 tagaggagag cttcaccgct accctgcgca ggcgcatccg ccagggggag ctggatgcca
      181 tagtggtggc gctgccgttt accgagccgg atgtggtgac ccaacccctt tatgaagagc
      241 gttttgtggt gctcatggcg gccaaccacc ctttggctca gcaggaggcc atagcgccgg
      301 atgccctcac cgactacaac ttgctgttac tcggcgaggg ccactgcttt cgcgatcagg
      361 tgatccaggc ctgccccaat cttaaagacc atattgaaga ccccagcggt agggtgcgca
      421 ccgcctcaga gggcagttcc ttagaaaccc tcaaacacat ggtcgcctcg ggtttaggta
      481 ttacgattct gcccagcacc gccgccagcg gcccccactt ggcggagggc ctcttggtgg
      541 ccaagccctt taccgacccc gcgcccaccc gcaccgtggc tttggcctgg cgggcgagtt
      601 tccctcgctt aaaagccatc gacgccctgc gccagtccat cgcccgctgt catttgggcg
      661 ctgtgagccg gggcgcttaa gcccatggct gctcgcccgg ctgtgatgcc cagtgcgcgc
      721 ggctctgggg caattgccgt gcgcggctta aaaggtgtgg gcgatgctat ggcgacgcgc
      781 cttgcgaagc tcggcattta ttctctgcag gatttatggt tccacctgcc cctgcggtat
      841 ttggatcgca cccagattac ccctatgggg gagctgagcc tcaacagcag tgtggtgatt
      901 caaggctggg tgcattcggc ccaggtgcag cagggccgca ggcgcagcct ggtggtggta
      961 atagaagatg actccggcca ggcggtgctg cgcttttacc actttaccgc ccagcaggtg
     1021 cagcgcttta gccccggcac cccgctgcgc tgctatggcg acccgcgctt aggggtgagc
     1081 ggtttggagt tttaccaccc ggagctggag tggttggccg atgccgaaga gctgccggac
     1141 ttggcgcaaa ccctcacacc catttacccc ctaacagaag gcatcagcca aaaccgtatg
     1201 cggcagatca tggcggcagg ccttgccctg ctcgagcagt acccgccgga agaattatta
     1261 gccgccgaga tcaaccagcg ttttggagtg gcgaacctgg ctcaggccct gcgttttgtg
     1321 cactgccctc ccatagaggc ccaggtgccc gcattgctgg caggcaccca tccctaccag
     1381 cagcggctgg cctttgaaga gctgctggcc cacaacctgg ccatctggca atcccgcctg
     1441 caggccaaac acgagcaggc gccggttatt gccccgccgg gaatgctcgc ccgccagctg
     1501 ctgcacggcc tgcccttcgc gctcacccaa gctcaggagc gggtgagcga tgagatccgc
     1561 cgcgacatgg cccaaagtgc ccccatgctg cgcatggtgc agggcgatgt gggttcgggc
     1621 aaaaccctgg tggcggccat ggcgtgcgcc caggccgtgg aggcaggcta ccaggtggtg
     1681 attgtggccc ccacggagat tctggccgaa caacacaagg cgaattttgc tcgctggttt
     1741 gagcctttgg gtgtaagtgt ggggtggctg gcgggcaagc tctccgccgg gcaaaagcgc
     1801 agcgtcagtg agcagattgg cgagggcgcc ctgcagattg tggtgggcac ccacgccctg
     1861 tttgaagaac atgtgctgtt taaacgcctg gggctggtca tcatcgatga acaacaccgc
     1921 tttggcgtgg cccagcgctt aagcctgcgg ggcaaaaacc ccgatggcgc cttcccccac
     1981 cagttggtga tgaccgcaac ccccattccc cgcaccctgg ccatgaccca atacagcgat
     2041 ttggacctgt ccatcatcga tgctctgccc cccggtcgca cgcccataaa cacggtgttg
     2101 atcaaccaaa ccaagcggcc agcggtaatc gagcgggtgc gcgctcaatg tgaggcgggg
     2161 cgccaggcct actgggtgtg cacactggtg gaggagtcgg aaaccctcgc cgccagcgcc
     2221 gccgaggcca ccgccagcca attgcaagcg gcgctgcccc agctgcgcat cggcctggta
     2281 cacggccgcc tgaaaagccg cgacaaagaa gccgttatgg ccgcctttaa ggccggtgag
     2341 ctgcatttat tggtggccac cacggtgatt gaagtgggag tagatgtgcc caatgccagc
     2401 ctgatggtga ttgaaaatcc cgagcgtttg ggcctggccc agttgcacca gttgcgcggt
     2461 cgggtagggc gcggctcggt ggccagccat tgtgtgctgc tctacgccga cccgctctcg
     2521 cgcaatggcc gggagcgctt ggaggtgatg cgggcctcca gcgatggttt tgtcatcgcc
     2581 gaaaaagacc tggccctgcg cggcccaggt gaactgctcg gcacccggca aaccggcgcc
     2641 ctgcaatttc gcatcgccca attgcagcgg gacagcggcc tgcttgaatg ggtgcaggcc
     2701 tgcgtggccg aggtggcggg tacggacgtg gaacagcggc tgattcagcg ctggtttgcc
     2761 cgccagcagg ggtttgtgaa tgcgtgaggt gctgctttat tgtggccgac atgggtaaac
     2821 gaaggatcgt ctctgcgggc agggcctaaa attcccctcc ttcggagggg tgccccgcag
     2881 gggcggggtg gtggggcctg gtcgaggttc tggccagtaa caccctgcca catgggtaca
     2941 aaaatagtgt ctttaagcag ggaatttccc ggcccccgcc cgccgcaacc accccggcag
     3001 gcgcgtctgc cacccctcca aaggagggga attttagggc ctgcccgaat cctaaaggca
     3061 cccttgtgtt tgcctggcgt ctatcgatcg agagttacgc gggagtgggc acccacaagg
     3121 ggcgccccta cggctgcgta tttgccaatc ccttaatcca gtgacgcccg ccaaggtggg
     3181 aatttgcggt tcgcccaaag agagtccgtc gattattcat ttaacaccgt tgcttttaga
     3241 aggctgaggc tggtgggcgc gatcttttaa gctgcgagga aataattaat ggccattgca
     3301 tggcggctgg aggcaacccg gatttcgctg cgctgtatcc aggctgtgag taaacccacg
     3361 accaccctga gcttaagaag cggcgccatt ttcctcccgt aaatactcat cctttaactt
     3421 gcaataattt tgtgccgaat aggtaaaaaa cgcccgttcc gaatccttca agggccgccc
     3481 ttgtttgcac ggattgccca catacaaaaa gccgctctcc aagcgtttac ccggcggcac
     3541 cagggcgcct gctgcaatga taacttcatc ctccaccact gcgccgtcca acaccgtggc
     3601 gcctatgccc accagaatgc gattgcccag ggtgcagccg tgcaaacagg cgccgtggcc
     3661 gatggtgacg tcctcgccga tggtcagcgg ccaaccgtcg gggttgaatt tgctggcgtg
     3721 ggtgatgtgc agcaccgcgt tatcctgcac gctggtgcgg gcgcccacgc ggatgcggtg
     3781 catatcgccg cgaatcaccg cgcagggcca cacggagaca tcatccccca acaccacatc
     3841 gccgatcacc accgccgctt ggtccacaaa cacccgctcc cccactgtag gttgaacacc
     3901 cttaaaactg cgcactgaca tatccacctc acgaataaac tgagcctgtt aaggctactg
     3961 cggctgtatt ggagcagacc cgttagaacc gaccccatag aagcgactat gataggccat
     4021 gatcccaccg ttattggaag gcaccatgac taatcctctg cttgtacccc aaggcctacc
     4081 ggccttcgcc cagatccgcc cagaacacat caaccccgcc atcgacagca tcatcaacgc
     4141 taacaagcaa cacctagcca cgcttctaaa cgagttaagc gcccccagct gggccacgtt
     4201 ggcagcgccc ttagaagaag ccgaagaccg tttagataaa gccttctcgc cggtaagcca
     4261 tttaaattcc gtggccaata caccccaact gcgcgatgcc tacaacagcg ctttgcaatc
     4321 cctcaccgcc taccacacgg ccttggggca gaataatgag ctctaccaag cctaccaagc
     4381 ccttgccgac agcaacgaat ttgcaaccct ctcccaagcc caacaacaag ccattaattt
     4441 agccctgcgg gattttcgtt tatccggggt gagtttagcg gcggagaaaa aaacgcgttt
     4501 tgccgagatc aaagcgcgtt tatcgcaatt aaccagttta ttcgccaacc aggtgttaga
     4561 cgccacacaa aactttttcc acaccattac cgatgaagcc gaacttgctg gcctgccgcc
     4621 tttattaatt caacaggcca aacaattagc tgagcaaaaa aacagccccg gctgggtttt
     4681 tagcctggat attccggtgt atttacaaat tatgacccag gcggaaaatg ctaatctgcg
     4741 gcaattattt tataccgcct atatgacccg cgcctcagat tgcggccccc aggccgggca
     4801 gtggaataac caaccggtga tggaggaaat tctgcaactg cgcttggaaa tggcccagtt
     4861 attaggcttt aaacactacg ccgactattc cctagctagc aaaatggcga gctcccccga
     4921 acaagtgttg gaatttttat atcaactggc gcagcaatcg cgccccatgg ccgagcagga
     4981 attagccgaa ttaacccagt gggtgcaggc cacttacggc aaaactgatc ttgccgtgtg
     5041 ggacgtaccc tattacagcg agcggttaaa agaagcgcgc tacagcatct cccaggaatt
     5101 tctgcgcgaa tatttccctc tgccacgggt attaaacggt ttatttagca ccatcgagca
     5161 attatttaaa gtcagcatcg cgcctatgca cagcgaggat ttatggcaca gcgatgctgc
     5221 cctgtacggc ctgtatcgag acggtgaatt aattgcccaa tgttatttag atttatatgc
     5281 gcgggagggc aaaaaaggcg gcgcctggat ggggcagtgc caggtgcggc gccaaaccca
     5341 agcgggcctg caattacctg tggcgttttt agtctgtaat ttcagcgccc cggtgggaga
     5401 tacgccttca ctattaaccc accaggaagt gaccacccta tttcacgaat tcggccacgg
     5461 cctgcaccat atgctcagca aaatagacgt agccgcagtg agcggtatta acggtgtggc
     5521 ctgggatgca gtggagctac ccagtcaatt tttagaaaac ttttgttggc aaaaatccgt
     5581 attaaaagac ctctccagcc actataaaac cggcgagccc ttaggggatg aatggctgga
     5641 taaaatgctt gcagcgaaaa attttcaatc cgccctgcag atgctgcggc agttggagtt
     5701 tgccattttt gatatgcgcc tacacatgga gtatggcagc agcagtttta ccagtgtaca
     5761 gaatctttta gatgaagtgc gcaaacaggt ggcggttatc attccgccgg aatttaaccg
     5821 ctttcaatta tcctttagcc atatttttgc aggtggttat gccgctggtt attacagcta
     5881 taaatgggcg gaagtgctct cttcggatgc ctttgccgcc tttgaagaaa acggcttatt
     5941 tgatagcgcc accggggaga aatttctgca gtgtatttta gagcgcggcg gcgcggtgga
     6001 agccatgcaa tgttttaaag attttcgcgg ccgcgaaccc agcatcgaag ctttattgcg
     6061 ccacagtgga attcaccatg acagcaccca ttaaaaaacg ctttatcgcg ggcgccgtat
     6121 gccccaaatg cgcaagcatg gatacggtat ttacctaccg ctttgatgat aaagattggc
     6181 gcggctgcgt aaactgcgat tttaacgaag ccatgatgtt tacgccgcag gtgcgtgaat
     6241 tggaaacccg cgtgaatcta gatgaagcca ccaaggcggc acaaacccag gtgatcaaaa
     6301 ttttaaaata atcctaaaaa ccgcagggat tgcgcctggc aggttaaaaa ctgtcacgcc
     6361 tgccacaaaa ccattaaaaa accttcaaaa ttccgtcatt gcattttcat gatgtgtccc
     6421 tagcctcggt tgcggcagct atcgctgtgg ccgcagcgat gcgtgctcct ttgttgttaa
     6481 ctagatttta gcggagacat gcaccatgaa taaaaataaa ctgacactag tattaagctt
     6541 aattttcgca agccaaggcg tgttggccaa caacaccact tttgatgaat tttcaccgct
     6601 catcggcagt gtgcccgccg gttcactacc ggaaacagcc ccttttaaat tagccaatcc
     6661 cgcttttacc caacaggctt ttgttatcaa caacagcccc accaactacg gcgataactg
     6721 ggatatgatc gacaccaacc gcaccggtgt ggatgccggc cgctatttat ttactccata
     6781 tgaaaccggc tcagccggtg ttatgcgggt ggatacctgg accaatacag cacaaaccat
     6841 cgtagcgccc ggcactcagg gttttgtctc cggcgatgcc tctcgttgga cgccctgggg
     6901 cagctattta acggcggaag aatcctgggg taccggcagc accaagggcc gtttatttga
     6961 agtgaccaac ccacttgctg ctgtgggcag tattaatttt gttaaccgca ctacatcgct
     7021 accgaacatc tcccatgaag gtttggcttt cgataacgcc aataatttat attttattga
     7081 cgaagtagac ggcggcgcgg tgtataaatt tacctccacc aaacccaatg ccagcaacgg
     7141 caacgatttt tttgccgccg ggcaaacctt tgcccttaaa gctgatgccg gtaaaaccgg
     7201 tgctttttcc tgggcggcat taaccgatgt taacggcggt ttactgcctg ccaccgctag
     7261 cgccgattta gccagcagct catttgacgg ccgtaaagcg gcggatttag tgggcgccac
     7321 caattacaac cgcccggaag atatggacat tcaacacggc gttaatggcg aaattcttta
     7381 tgtggccacc accaccgacc acgaagtcta tgccattaat ctcagcacca attcagtaag
     7441 cctatttgcc agccgcaata ccattaacct cgccacaggt gttgccgtcg gcactgcatt
     7501 taccaacccc gacaatatag ctatcgacgc ggatggcaat atttatgtag tggaagacca
     7561 acccggcggc tcggcagata tttggatggg caaggattta aataaagacg gcgatttaac
     7621 cgacgcaggc gaaggcttgg cgcgttggct ggccctttct accgtgggct ccgaacccac
     7681 tggtttgtat tttgatgtat ttaatccaac ggttgcctac gttaatgtgc agcacgccga
     7741 tagtgataaa gacaccatga taaaactgac ctcacccgcc cccgtgccac tgcctgcggc
     7801 cgtgtggctg ctcggctctg gtatgctcgg tttaatgggc gttgcccgtc gtcgttcgaa
     7861 ggcttaagtg tgcggtcagc agggatgctg gtttttaaaa ttgcagcgcg gctttttaag
     7921 ccgcgctttt gtgtgctgtt ctgcgtacat tgatagccgc aaaataaaaa cctctagtta
     7981 caactcctta tctgggttcg gctttcattg tcggtttttt gcttaagtcg gcattaattg
     8041 gtgtgtaacc agggttgtga taaatatcag cggcttcatc ataatagccc cccatttcaa
     8101 ccgtcagctg attgttttta gctttaaaat tggccttgcc taaacggcca cacatttggg
     8161 ctgagaagaa tttagctcaa atctaaactg acagccaccc ttgaaaaatc ggtaactgta
     8221 agatcaggtc gagactaata caagtgattg ttggctgtat gacctcaagg cacagctggc
     8281 agtacgaaga cgctattacc cacgatgaca ttgcaattac cagcccaact ttcaccctaa
     8341 tatcagaaaa ccccgcagcc aatatcggaa atgcgcgatt caaccctgag gaacaaaaaa
     8401 ttattttgtt cagtggttca atcagaacta cgacgcaaat tgtagctata gaagggagcg
     8461 tggagactac taaatagcat ttattggtgg cgtcctaccc ataggaataa aactgcaatc
     8521 gccgccggag ataacagaat taatcacaac caaaatgatg gcctgtattg cgctgaataa
     8581 ggatctctac attatgatca gctttagcct ggattccctc cactacgaca atcgcaaaaa
     8641 ccacaacagc gaagaactcc tccatcaatt taaaattgat taattcgatg agtttctctc
     8701 ctttatccag gctacttatt ccgttgaagt ttaaaaatta gttgaacaac aggcaataat
     8761 gtaaattcca cgccccacta gcgatcagct aaacatctgc gctgcgcaac catttgctcc
     8821 tcagcctgcc gtgccaggca gcacatcagc aaagactgac actggcaagt ccagcaacac
     8881 gcaggcagct gtgctgcccg caaaatactg ccgctagttt cctccgcgag gggagtttgt
     8941 tatggtggtt gtttgccata ggcctaggag ctaaccatga gctttataag tgttgaatcg
     9001 cgcgtaagcg gcaaacccaa tagatcgaac catacaagcg agttttcggc aggcgagttt
     9061 tcggtcgtca tcttgggtga tttctccggg cgcgctagcc gggcagtaag cttaccgctc
     9121 agccggttag tgcccgttga tcgcgacaat ttttttgact tatttagcgc atttgatatt
     9181 aagctcaaac tgccatttga tgagcagcca atcgaattta aagattttga gcatttacac
     9241 ccggattatt tgctggacca attggccata ttcgccaaat tccgtcagtg gcagcgtaaa
     9301 gcgctcaagc ctgaacattt tgaaagcctg ttggaagaac tgcgggcggc taaggtttgg
     9361 gatgatcagg cggcaagtgt acaaactgat ctgcaggcca atgcccacac atctgctgaa
     9421 aaagccgata tgagtgccga ttttttattg gagcaggtgt taaaccaagg ggttgagggc
     9481 tcggcgcaga ccgatgtaga acaattaatt cgctccatag tcgcccctta cgctacctta
     9541 aaagcagata ttcgcctaag cgaagtgcaa aatgctatta accaagccat ggcgcaaacc
     9601 ctgcgcagca ttttgcacca cgggcatttc cataatctgg aagccacttg gcgcagtttg
     9661 tattttttcg tgcgccgcat cgccagcgat aacggtatta aaatctggct gctggatatt
     9721 agcgaggcgg aactggcgca agcgagccaa gaggagccca cacaatccgc tctttacgat
     9781 ttattggtga aacagcgcca ggcggcaggg caatcggccc ataatttaat tattaattta
     9841 tttgctgccg acagcaacct tacgtcttta aattggctta aaactcttgc ggcgattgcg
     9901 gcagccaata atggcgcctg cgtgggtgca gcaaacccga acattgctgg ctgcagcaat
     9961 ttaattggca tgcccgaccc ttcgcagtgg caggggccgc ctgaggactt tgccactgct
    10021 tggcaatcct tccgtgccag tgctgacgcg gcccatctgg ccctcactgc gccgcgtttt
    10081 ttactgcgca caccctacgg caaaaaatca tcccccattg aaacctttga tttcacagaa
    10141 atgattactg aaggtaacca ccagcattat ttgtggggca atagtggtgt tttagtgggt
    10201 gtggtgttgg cagagctgtg gcttaatggc aagggcgtta ttaattctgc ggcggcaagc
    10261 caaattgacc agctgcccat acatgtggaa aaaaccgcag caggagagca attaatcccc
    10321 agtgcggaga ttgttattct agatagtact gttgcggcat tgcgggcagc gggtttattg
    10381 gtggtgcgtt ctatttacaa taaaaatgcg gtattaatcc cgctattaaa tagctgtgcc
    10441 gccgatggtg cactgttata cagccgttaa actttaattg tagagaaaat aaaggcggcc
    10501 atcattcaga tgggccgcct ttttgttagg gaacctctga aaaatgtagg cggcgaagca
    10561 ctagctaggc gaatttggca aaaaaagcgg agtttacacg cagtaaatga gcattttttt
    10621 gccaaattca acgacgctag ggcgagtgag gtaatttttc agaggttcct tagggccttt
    10681 taaggtaatt accagtctac acttaagcct attttcatag tgcgcatagg gcctgcgata
    10741 gtgttatcac tggtgccgaa gttgtcgggc ttctgctcgc ctgcgccgcc aaaccagtca
    10801 ttaaagccct tgtcgttgcg ggtattggcc aggtttaaaa tatcgaggcg cagcaccaca
    10861 cccgaaccaa aataccgcgg gtcatcgata atggtttttt tcaggctgat atcaaattgg
    10921 ttgaagccga ataattcacc tgtatcgggg gttgcgctgg cgtatttaca atcctcccag
    10981 cccgcacggc aatcgatgcc ggtaatgggt ttttggcttt ccaacttcca tttagccgac
    11041 agggtaaatc cgcgcggcaa aaaataactg ccggccatca ctaacttatg ctctggcacg
    11101 ctggtggccg gtaaaaagct gtaatcggag aatttagggt aatctaaggc gaaatattcg
    11161 cggaatttac ggttttcttc cgcatcggtg taggtgtagg ctgcggaaaa gctccagtta
    11221 ctttctgcgc ggggtttttc cgcctgcaaa tagagggact gattgcgggt ttctaatccg
    11281 ctggcgccaa ttaatgtatt accgtagccg gggataccat tgccccaagg ggtgccccag
    11341 gttttaccgg ggttaaagaa gttgccatcg gcgcggcggt tggcgagtag ccaagcaaat
    11401 ccatcctgag tggtaatatg cgcgatactg gcctgcaaat tccaatcgcc cactgggctg
    11461 cgcaccccta agctgaactg gtcggaataa ggcactttta attcgttggg caataaatat
    11521 aattcgcgcc cggcgccatc gctgttagct aaagcataga gattatccgt cgcataatat
    11581 ttattatccc aagcaataca attggcgctt agggttgcgc aggcgcccgg tacgccaggg
    11641 gcaataaagc gatattcata ggtagggtat gtcgccttag tggtttctag ctgcagataa
    11701 tcaaataaat tgcgatcata ggcccggcct gcaccgccaa ataactccaa accctcatcg
    11761 tttaacgcgt aggtaaagcc cacgcgcggc tgaatagcat ctttaaatgc actgcggttg
    11821 tggccgttgg caatatagtc attaatgggt acgtcggatt ttttaatagc gtcccaggaa
    11881 ttaatgccgc tgactacttt tgctggagtt acataatcca aatacacgtc ggattcttca
    11941 tagtcccaac gcaggccgag atttaaagcg agggcgtctg tcgcacgcca gtcatcctgc
    12001 aggtatattc cccactgtgt gttatccgcc tccacactac cggtgcctat gtctttcagc
    12061 ggcgcaccaa aacgcacgcg attgggctga ctgacggcac cttgataata ataaaactgc
    12121 ggattgtagg gctgctgttc ggtggcgtgg agcgttactg ccttgtattt aaggccggtt
    12181 ttaatattgt gaccaacaaa accggagtag gtgaaatcct cttgcaggcc caagcctttt
    12241 tggcccttgt cttgaaaatc ttcaccgcca cccaccttta ataattcttt ttgctcttcc
    12301 cgctcataga tttgcccata accattgctg tgggggttag ggttaaattg ggtgtcttcg
    12361 tgggtaattt taaattcatt taaaaagaaa tcgccgcgcc acttatggcc taaagccatg
    12421 cgcacatcgc gattgtcccg atcggtggcg tgctccagcg tattggtgcc acccacgccg
    12481 gcaagctcag tttcttggcg gtattttgcc gttaactcca acgcatgctg ttcattgacg
    12541 gtgaaatcca attttccaaa cacaaggtcc tctttaaaat cggccacaac tccaccgatt
    12601 tttgccccca agctggcagg cagcttagta aagtcagcgc cgtcacctgg caccacctct
    12661 ttggggtctg cataattttt cccttcatag gtgataaaaa aatgcacggc atcttgaata
    12721 atagggcctg aaatattcgc gccgaattga tattgattgc tgggtgtttt atagcggtcg
    12781 agtttttcgc gcggcttttg tgcgcgccaa tggtcggtgg tgtaatcggc aaaaacaccg
    12841 ccttttaatt cattaccacc cgattgagtt accgccacta ctgccgccga gctaatttgg
    12901 tcaaattccg ccgagtagtt ttgggtgata accgtatatt ctttgatggc tgactggggg
    12961 aatggatcgc cccggctgga atcttgcccg ctcaccccgc cctgcaatac atagtctttt
    13021 tggccaacgc catcaataaa gacattgata ctgctgggcg tgctggcgcc tgcacgcata
    13081 ctgctggtgc cgtcatcttc ttgcacaaaa gcaacgccgg gcgccaaatc ggctgcgctt
    13141 aaaaaattgc gattggtttg cggcaggttt ttaatttgtt cagtagaaat ttgtgcgcct
    13201 atggcgccgc ctttgctggc tttttcaaca attcccgtaa caaccacttc ttccgtttgg
    13261 gtattttttt catcaaatac cacctgggta atttgcccca cccgcaaccg cagcagttga
    13321 ctggcatccg agccctgcaa tttaagggag taattgcctg gcgccaatcc gtttaaaata
    13381 aaatgcccat ctgcttgggt gcgcgttgta ataacacggc cggtatctac attggttgcc
    13441 tcaataatct gcccggcagc ccgggccgtg cgcacctcac cttgaatgcc cgcatcacta
    13501 acatcggctc tggccgcctg ggctaacatc acgcacacta cacattgagc gaggattttc
    13561 ggagtaaatt tcaaggtgtt tagttttatt gacataatga ttcctaatga ataaatagga
    13621 tgctctgatt aagtgtaaat tcgcgaatat tttgcaaaca cacactttcc tgtagctttg
    13681 gcgcgagcct actgcccgcg acattttcaa aacatctcgc cgtcagactt aatcagagat
    13741 tccaatatgg acgtaagact atgggctgca catgaattac tcggcagcta acagccggtt
    13801 aaaacatctg ggcgccggtg gttgggataa agttggcgag ccttatttgc acttgggcat
    13861 acagagcaca aaaatgcgcc gcctaattgc agctagaaaa gctatgttac gggagtgcgc
    13921 actgattgcc cgcccgcatc gcttggctac acctgaacaa cgataacaac actcagccgc
    13981 cacggattgc atccataact ggctaagctt tgcctaacca tagctccaga agccttatcg
    14041 gcggcgcgca gtataaacaa ttacagccat atgaaaacac gttaatgacg tgtgataaaa
    14101 gctggggggc ggtcatattg tgccatcaac acgggcggcc aagccgctga agatatattt
    14161 gtgggcctgc gcaataatca caaatgtaga tgtcacaagt tactcaaata cattagtgac
    14221 aacttatttt aaccggttgt tgggcgcatg ttataaaagc aggtaaatat tggggcttag
    14281 ctcgctggcg gatgagagta acggcttttg aaaatattat tattaacccg gcggcattta
    14341 gtgccgggtt aataatgtcg gcatggatgc cgcgataaag gtgcgactcg ccgcgagctg
    14401 cactaaaaca agaaaatccg cgcattttcg caagtgtcag tgc
//
LOCUS       DAOQGS010000027        14391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_1935_length_14391_cov_4.534668, whole genome shotgun sequence.
ACCESSION   DAOQGS010000027 DAOQGS010000000
VERSION     DAOQGS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1935_length_14391_cov_4.534668"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1401..1565)
                     /locus_tag="PKZ52_01860"
     CDS             complement(1401..1565)
                     /locus_tag="PKZ52_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58539.1"
                     /translation="MNRPLCCYFSLSYNTDSACWPEVAHRLASQYPTKTLKLRAASYP
                     PQTPNTRQAD"
     gene            1564..1692
                     /locus_tag="PKZ52_01865"
     CDS             1564..1692
                     /locus_tag="PKZ52_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58540.1"
                     /translation="MAKRRVLIVADSKTAQVRLRKMLERFDVDVTSIFKLRGDTDF"
     gene            complement(1818..2222)
                     /locus_tag="PKZ52_01870"
     CDS             complement(1818..2222)
                     /locus_tag="PKZ52_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG58541.1"
                     /translation="MPLKYILLSFFILLQTGCSCFTPEAEHGLKKISVAEVSKDIYGG
                     SPKFVILDANPESVRSSKGTVPNARLLSSYDQYRLDELPADKTTPLVFYCFNEACGAS
                     TQAANRALTSGYKNVSVMEGGITGWNKEHPRN"
     gene            complement(2317..3699)
                     /locus_tag="PKZ52_01875"
     CDS             complement(2317..3699)
                     /locus_tag="PKZ52_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNG58542.1"
                     /translation="MLKILPLIFLFSTATAFASSDEESNMSVEISEIADGIRSINDSD
                     YNKVDAAKVTLGQALFFDKELSGNRNISCSTCHSPITATTDGLSINLGEGAKGLSVSR
                     DPGKFPPEASAPLERGQRNAPALFDRGAKEFTVLMHDGRFQVDKKQPSGFLSPAGDLL
                     PLGFNNMLEVISIFAFTEIQEMLGQVGENEVADAGAAQPFTGIWSALVERIKKVPAYK
                     PLFAAAYPELGGDINKITIVHVGRAIAHFQSASFRASGEAPFDRYMRGDNQALSKEAV
                     RGLKVFVGKGNCVSCHSGPFFTDHKFHALGVPQIGPGFGGVGNDQREDFGREGVTQNA
                     SDRYKFITPPLRNVALTGPWGHDGAFNSLEAIVRHHLEPKKSLLNYNPEQRVLPSRED
                     LDKLDLIALMNPTTTSQIASRIEINGIRLSDSEFKDLMGFLNALTDPKAVDLRKTVPK
                     KVPSGATLAD"
     gene            complement(3725..4891)
                     /locus_tag="PKZ52_01880"
     CDS             complement(3725..4891)
                     /locus_tag="PKZ52_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNG58543.1"
                     /translation="MTCLHENLSALDKFYEEINSSIIDRDAKGEPISKAVFQRCTELG
                     LYSFGIHSSRSSCSPITWGKHLEQLGYRCLDSGLALLISLRAGLANTILSANKSALNH
                     AYQEGLRIGSIVPAFAYTDGADPFSFNTQAHKVEGGYVVNGKKLYVTGGATADLFVVY
                     ANKSIGSGTDLGVFLIQKESKGLKTEIADLAGLRTAGIATLILENCFVPIDHVLQEHD
                     GLSHVQKFLNERRLFLVSPLIGQMRKILEHTIDELSERIRYNAPLTSMQFVQAQLGRM
                     YTLYETAKASLYSGLLRQASSDFDTYWDPVNSSAKLFIVEAAIELVQISQRLLGGDGY
                     LRSQHVERFYRDFAGYIPGGGSQDTLLVDLGINAITKRQMLAIERRFSQDTTIN"
     gene            complement(4905..6056)
                     /gene="moeB"
                     /locus_tag="PKZ52_01885"
     CDS             complement(4905..6056)
                     /gene="moeB"
                     /locus_tag="PKZ52_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNG58544.1"
                     /translation="MPTTECKLQPEELGRYSRHLLLSEVGIDGQLKLKNAKVLLIGTG
                     GLGSPIALYLAAAGVGTLGLVDMDVVDLSNLQRQVIHNTKTVGTLKVNSAAASIEKLN
                     PFVKVNIYNEKFSSANAIDIARGYDIIIDGTDNFPTRYLVNDVSVLLGIPNIYGSIFK
                     FDGQVSVFWPGKGPCYRCLYPEPPPDGLVPNCAEGGVIGVLPGMIGTIQANEAIKVIL
                     GIGHTLINRLLILDALEMTFRELSISRRHDCEICGDQPTIHAPIDYEVFCGIDRGKTA
                     ESAQNINCTELANLLEESSDLILLDVRTRAERQIAKIEGSIHIPLDELKESVEILDKN
                     KLVIIYCKTGVRGRKAQDYLITEGFERVFNLEGGIVAWSAQIDQSIAAY"
     gene            complement(6097..6813)
                     /locus_tag="PKZ52_01890"
     CDS             complement(6097..6813)
                     /locus_tag="PKZ52_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58545.1"
                     /translation="MREHVVSIQKMIAEYSASVVVAGCSLEYKGPCLFHDGEVINIRT
                     HSSVLKKGILIQGNSDFFLDSHPLASFKIYFRPVNIGDHTSSAATSGHLNPDIQSLFT
                     PEERSDLMPQRFVKQNMSKIESEGMLIATHQEPFRLNRYAMDFADQWAFIESAAFCSV
                     SREALAISGGEHFDKLVTGISKPLQSYHLDLKKPYFLLDEGLVDTKVYILRDDIFYVH
                     RLMGTNNELHAVAIEQFNYK"
     gene            complement(6925..7191)
                     /locus_tag="PKZ52_01895"
     CDS             complement(6925..7191)
                     /locus_tag="PKZ52_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNG58546.1"
                     /translation="MNLAFSGTLLRFVNFQKNLHIEADSIEDALKKLANDYPQSAGVL
                     FDDAGQVRKVHQLFLNGKQVDPAQLSSPVKDSDRLDVLTAIAGG"
     gene            complement(7200..7766)
                     /locus_tag="PKZ52_01900"
     CDS             complement(7200..7766)
                     /locus_tag="PKZ52_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58547.1"
                     /translation="MKKIIAVSAMLLSCSSLSISVSANEEEVSAYKKLKGCYTVVRAT
                     MEESSVDASHVIGTYKFILVPKEGKKKTLTISGPVSGAEASGSHEEGESDGEIHGGHV
                     FGTKNRLGTFSSGGDEFEVTSAKCPDAEGNPKLIEGIETISFEKGTGIFSGLKKGIIP
                     FNTTFDACTDPSNPVGDLYATSGELCFE"
     gene            complement(9011..9241)
                     /locus_tag="PKZ52_01905"
     CDS             complement(9011..9241)
                     /locus_tag="PKZ52_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58548.1"
                     /translation="MANTTLMITTGASLQRDERTVRIMRIIKEERKNWYGPFFGRHQL
                     PRRKLAPYSLLKYYLARVGGVKFGEKSLIYCL"
     gene            9432..10832
                     /locus_tag="PKZ52_01910"
     CDS             9432..10832
                     /locus_tag="PKZ52_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58549.1"
                     /translation="MAKRRVLIVDDSKTAQVRLRKMLERFDVDVDTVNSAEEALGYLS
                     YRQPSVIFLDHHMEGMDGLAALKIIKANPGTALIPVIMYTSEQGDVYVGQARALGALD
                     IISKEVIKQVDLEKLMGSLNIYLIGAEPPEAEQVPHASTLDIVADLEEMPIAPAAASL
                     DELPPANDLARVQLQIGRLFEIHIAKVRQEIEDSTKFLMRRLSKEIQERSAKPYVRPV
                     TPVANPSNEPLPPIEVESEKERGISTFILALVLLGVAFLGYHLYINNAHLQLIAEKYD
                     VLQQQNAKQEALINRLFEQSVQGKAAPELDTNMMLETLSWASNMNNQVAFGEQALNDQ
                     RIYPVGELVGVLKAANFAGTVYLDIHLGNFCVVEDPSGRYILPEPESNIEDCVFLADK
                     QPQVPLDSHISVGFLNYVHSAPVLTEGPIELEFAMQGYSQPKASYPPKTSGTSAGEWN
                     EAARKNNRVSFSFARK"
     gene            complement(10900..12399)
                     /locus_tag="PKZ52_01915"
     CDS             complement(10900..12399)
                     /locus_tag="PKZ52_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HNG58550.1"
                     /translation="MSQPLTSITILGGGTAGWLAAAILSQEIGEQCHIHLIESPDIGT
                     IGLGESTVPPFVGLIKSLGLDETLFMQQTDATYKLGIEFVDWRVKHSRFFHPFGSTGA
                     KILRQEFYPCWLKAQEHGPVAPLEDFSPCQVMAKEARFFPPQLAQHTPIGGANYALHV
                     DAEHLVAALKQRCASRPFSAQAARVVSARQQDNGFIEALILDDGSEHKADFFIDCSGF
                     HSLLFSQFLKVPFCDWSRFLPCNRALATRAPSQARPHLYTQAKASLAGWRWAIPLRSR
                     TGMGYVYSSDFISDDKALVELCQAANLAPNEQQARVLPFATGHRQVFWQHNCLALGLA
                     AGFIEPLEATAIHLITRGLDFFLRFLPHKQCDPSLARAYNLRMNKDFAEVRDFVQLHY
                     VKSQRQDSAFWRYCQSLPISDSLHERMDLFTAQGLIDERSDDLFRPSSWLAIFEGMGI
                     RPRHNSPKVAHLSAELLHQELTKTAAAINTMVKTLPRQEEFWQSAAQAQ"
     gene            complement(12431..13366)
                     /locus_tag="PKZ52_01920"
     CDS             complement(12431..13366)
                     /locus_tag="PKZ52_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58551.1"
                     /translation="MTLNELRFFIALAKTQHFGKAAALCHVSQPNLSMGIKKLEAALG
                     LELFERSKLGVRLLPQAEPLLQCAQAALGQVQTLKDLAQQMGDHLHGPLKLGVQQGLA
                     PEWLPQVLGHLKWLAPHMPLVLQEAEAGALVANLRTGEIDTLLNCQLIEAPDTVSLKL
                     ADETYVALLPAQHPFHTATCLSLDEIAERLQIPAADSALVMSLQQLGLRSITLATVGI
                     ELLKQVTAADLAVTLLPKSSAQAAVAQNQFLAWLPVNPAPQRPLYLHWRSNFPRHKAI
                     DALKRALITSCSVFWDFTHWQEQDEPYSLADHSRW"
     gene            13387..13524
                     /locus_tag="PKZ52_01925"
     CDS             13387..13524
                     /locus_tag="PKZ52_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58552.1"
                     /translation="MPSSAGKGETRVDILPARQKINYTVRTTNTQPAVLIPGGPIYLG
                     K"
     gene            complement(13600..13947)
                     /locus_tag="PKZ52_01930"
     CDS             complement(13600..13947)
                     /locus_tag="PKZ52_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNG58553.1"
                     /translation="MPVYEYKCAEHGLFFELATMEEAHLPNQCPTCNKQSPRVIMVNP
                     ALFNLPPEVKKAHALNEKAQHAPEFSTKARREDDHQHKKGCGCQNKKSRTLLYTAQGE
                     KMFPSARPWMISH"
     gene            complement(14051..>14391)
                     /locus_tag="PKZ52_01935"
     CDS             complement(14051..>14391)
                     /locus_tag="PKZ52_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HNG58554.1"
                     /translation="ITPKYDDYLIFEGISVDEKGKQHYLDVHIAYRQACLNAIEYMTK
                     FGYSKAQAYAILGTAPVQGHISGVVDIPNACATLWLPTDIFAFDIKPNGDGPKKMVDG
                     SVDIPLSKDK"
BASE COUNT         3636 a         3328 c         3592 g         3835 t
ORIGIN      
        1 aatacatcca tgtaggctcg tcaaaaaggt ccctcttttt gacggtctgc actaaccggt
       61 agtcaccact gctacaccgc gaatcgatta tgccgaatgg ctaaatcctc ctgaaattgc
      121 cgcccgcgcg gaaaggggga gtaaagtgtg ctgaatagtt gcaaaacttt taatccgctt
      181 gcggtaagca gttatgggcc ggtgatatta cctatctgaa aacggctgaa ggctggatgt
      241 gtttggcgat tgaggtggat tattaatccg gcggcaaaat agatcgtcct gatctatttt
      301 gcctcggctg cactgacact tgcgaaaatg cgcggctttt cttgttttag tgcagctcgc
      361 ggcggttagc gcctttggcg cggcataatt gccgccgggt aaataatttt ccgcggattg
      421 tgggctggaa tattgagctg tgtataaatg ccgattgagt tggcaaggtt atgatcgaag
      481 tggtgaacct cgccaaccgc ctaacagatt attttaatgc tgcctgccag ccgttgctgc
      541 ctgcattttc agagattcct caggtgttac acgctggatc ggttgcacac agctagcggt
      601 gctgcgtcgc cgatcaaatt tgcaaacaat aaaattaaat agtcggtttt agcttgccaa
      661 tatattgctt aacgactttt ttcgaactat tagccctgcg gccctttatg ccaggttaat
      721 taggctagct gcgccgcagt taaaactcaa ggttcgcagc tgcctgatgg caaagattat
      781 tgaggccttt gcccttttca agctcgataa gtaggtgaga taagtcacat tttggactaa
      841 aggctattaa cccggcgccc caataaatcg tgatgattta ttggggcgcc gcttgtttga
      901 tgctggcgaa aatgcgtgaa ttttcgtgtt ttagcgtagc tcgcagcctg atacgccgca
      961 tccttgccgc ccctattaac ccaatgtcat taagctaagg cggaagcgca gcggcaaccc
     1021 atgtggttgc ccagtgcctg cctcggctat cgattagggt ttacggtgga acgggcatcc
     1081 gcaacgggtg cccctaaggc tgttttcgag ttgggaaaac cataacttac tgcctaggca
     1141 ccggcaatga atacccatca tttcagacac gccgtgaata catcctaagt aggctcgtca
     1201 aaaaggtccc tctttttgac ggtctgaaat gatgggtatt cattgcttcc gtacagctag
     1261 taatgacttt gcctactaac tcggcaaaaa agctaacccg acataaaagg ccgccgggtt
     1321 gctaaccgag gtctttaggg taaagccagg ttggcctgct aagcttggca gagtattttc
     1381 tctgcaattg catccaatca ttaatccgct tgccgggtgt tgggcgtctg cggcggataa
     1441 ctcgcagcgc ggagttttaa ggttttggtg gggtattggc ttgcaagcct gtgggcgact
     1501 tccggccagc aggcgctatc tgtgttataa cttagtgaaa aataacagca taagggtctg
     1561 ttcatggcca aacgccgcgt actgatagtt gctgactcca aaacagccca ggtacgcctg
     1621 cgcaaaatgc ttgagcggtt cgatgtggat gttacctcta tctttaagct taggggtgac
     1681 acagattttt gaatactacc ttggagctga atgtagtact tgcaaattta aatatttggc
     1741 ttcgaaagag atgaatgaaa aactgcccgc cgccctgggc ggggttgggc agtatttgtc
     1801 aaatcatcga tgtacagtta attgcgcgga tgttctttgt tccaccctgt aataccgcct
     1861 tccattaccg aaacattttt gtatccagaa gttaatgcgc ggtttgcagc ctgtgttgat
     1921 gctccgcacg cctcattaaa acaataaaac actaatggcg ttgttttgtc tgcgggtaat
     1981 tcgtcaagtc gatattgatc ataagatgaa agtaatcttg cgttaggaac tgttcctttg
     2041 ctgcttcgaa ctgactccgg attagcatcc aaaatgacga acttaggaga cccaccgtaa
     2101 atatcctttg aaacttcagc tacggaaatt ttttttaaac catgttcagc ctcgggagtg
     2161 aagcatgagc atccggtttg tagcagtata aaaaatgaga gtaagatgta ttttaagggc
     2221 ataaaagtct ctgtgtttgg cttttggttg tgcaaacttt taataggtgg tgatagagat
     2281 acggggtgtg atataacacc ccgcctcaat cgttgattaa tccgcaagag ttgccccgct
     2341 gggaactttt ttaggaaccg ttttgcgtaa atcgactgct ttgggatcgg taagtgcgtt
     2401 gaggaaaccc atcaaatctt taaattcgct atcgcttaag cgtatgccat ttatttcgat
     2461 tcgagatgca atttggcttg tggttgttgg gttcatcagt gcaattagat caagtttatc
     2521 taaatcttct cgagaaggta gcactcgctg ctcggggtta taatttaaaa gagatttctt
     2581 gggttcaagg tggtggcgaa cgattgcctc tagagagtta aatgctccat cgtgccccca
     2641 tggtccagtt aacgcgacgt ttcgcaatgg tggagtaata aatttgtatc tatcagacgc
     2701 attttgggta actccttcgc gtccaaaatc ttcacgttga tcgttgccaa caccaccaaa
     2761 tcctggtccg atctgtggga cgcccaatgc gtggaattta tgatcagtaa aaaaggggcc
     2821 gctatggcaa gatacacaat tgcctttgcc aacaaaaact tttaatcctc ttacagcttc
     2881 tttacttaac gcttggttgt ctccacgcat gtaccggtcg aagggggctt cacccgacgc
     2941 acgaaatgat gcagattgaa aatgagcaat cgctcgccct acatgaacaa tagtaatttt
     3001 attgatatcg cctccgagct ctgggtatgc ggcagcgaat agcggtttgt aagcaggaac
     3061 tttttttatt ctttcaacta gagcagacca aattccagtg aagggctgcg ctgcaccggc
     3121 atctgcaact tcattttcgc caacttgtcc tagcatttct tgaatttcgg taaatgcaaa
     3181 aattgatata acttcgagca tgttgttgaa gcccaagggc aagaggtccc ctgcaggaga
     3241 taaaaatccg ctgggttgtt ttttgtcgac ttgaaagcga ccatcgtgca tcagcacagt
     3301 aaattctttg gcgccgcgat cgaacaacgc aggcgcatta cgttggcccc gctccaatgg
     3361 agcggaggcc tctggtggaa atttgcccgg gtcgcggctt acgctaagac cttttgcccc
     3421 ctcgcctaga ttaattgata atccatcggt tgtagcagta atgggggagt ggcaggtgga
     3481 gcatgagatg tttctatttc cgcttaattc tttgtcaaaa aataaagcct gacctagagt
     3541 taccttagcg gcatccactt tgttgtagtc ggaatcatta atggatctaa ttccatccgc
     3601 tatttcacta atttcgacgg acatatttga ctcttcatcg gaagaggcaa atgccgtcgc
     3661 tgtggaaaat aaaaatatga gggggagtat ttttaacacg aaattatcct cttttccgta
     3721 gaatttaatt gattgttgtg tcttgtgaaa aacgtctctc aattgctaac atctgtcgct
     3781 tggtgattgc atttattcct aaatcgacca ggagagtgtc ttgggatccc cctccgggga
     3841 tgtatccggc gaagtctctg taaaatcgct ctacatgttg actgcgcaaa tatccatcac
     3901 cacctaaaag tcgttgcgaa atctgtacga gctcaatggc ggcttcaaca atgaatagtt
     3961 tggcactcga gtttactgga tcccaataag tatcgaagtc cgagctggcc tggcgtaata
     4021 gcccagaata taaagaggcc tttgctgttt cgtatagtgt atacatgcgg cctaattgag
     4081 cttgtacgaa ttgcatggaa gtcaacggcg cgttgtatcg aattcgttcg cttaattcat
     4141 ctatcgtatg ctctaatatt ttcctcattt gacctataag cgggcttacc aaaaaaagcc
     4201 ggcgttcatt gagaaatttt tgcacatgac ttagtccatc atgctcctga agcacgtgat
     4261 caattggtac aaagcaattt tctaaaatta aagtggcaat tcctgctgtg cgcaaacctg
     4321 ccaaatcagc aatctcggtt ttaaggcctt tggattcttt ctgaatcaag aaaacgccca
     4381 aatctgtccc gcttccaatt gatttgttcg cgtaaacaac gaacaaatct gctgtggcgc
     4441 caccggttac gtataatttt tttccgttga cgacataacc tccttccact ttgtgcgcct
     4501 gagtgttgaa cgaaaatgga tctgctccat ctgtgtaggc aaaagctgga actatactgc
     4561 caatccttaa cccttcctga taggcatgat tgagcgctga tttatttgcg ctgagtattg
     4621 tgtttgccag ccccgcgcga agtgaaatca acaaggctaa accagagtcc aggcagcgat
     4681 aaccaagttg ttcaaggtgt tttccccagg taatgggaga gcaggaagaa cgagagctat
     4741 gtatgccaaa cgaatacaga cctaattcag tgcagcgctg aaacacagct ttagaaatgg
     4801 gttcgccttt cgcgtcacga tctataatac tgctattaat ttcttcatag aatttatcaa
     4861 gcgccgaaag attttcatgc aggcaggtca taattatatt ccaatcagta agcggcaatc
     4921 gattgatcga tttgtgcgga ccaagccaca atgcctcctt caaggttgaa taccctctca
     4981 aacccttctg ttattagata atcctgcgct ttgcgccctc taacacctgt tttgcagtaa
     5041 attatgacca atttattctt atcaagaatt tctacggatt cttttagttc atctaatggt
     5101 atatggatag agccttcgat tttggcgatt tggcgctcag cgcgtgtgcg cacatctaat
     5161 agaatcaaat cggatgattc ctctagaaga tttgctaatt cagtgcaatt tatattttgc
     5221 gctgattcag ccgttttgcc tctatctata ccgcaaaaaa cttcataatc tattggggca
     5281 tgtatggttg gctggtcacc acaaatttca caatcgtgac gcctagagat tgacagttct
     5341 cgaaatgtca tttctaaagc gtcaagaatt agtaaacgat ttataagagt gtgaccaatg
     5401 ccgagtatga cttttatggc ttcattcgct tggatcgtcc ctatcatgcc aggtaaaaca
     5461 ccgataacac cgccttctgc gcaattgggc actaggccat ctggtggtgg ttcagggtaa
     5521 aggcagcgat aacaggggcc cttgcctggc caaaacacgc tcacctggcc gtcaaattta
     5581 aatatgcttc cataaatatt cggtattcct aataaaacac taacgtcatt cactaagtac
     5641 cgtgtaggaa agttgtcggt gccatctatg ataatgtcgt agccgcgagc aatatcaatt
     5701 gcatttgcgc tggagaactt ctcgttgtaa atattaactt ttacaaaagg gttcaatttc
     5761 tcaattgatg cggccgcgct atttactttt aacgttccta ctgtttttgt attgtgaatt
     5821 acttgtctct gtaggttgga cagatccacc acatccatat caaccaagcc taaagtgcca
     5881 acacctgcag ccgcaagata tagcgcgata ggtgagccta gcccgcctgt accgatcaac
     5941 agtacttttg catttttgag cttcaactgc ccatcaatac ccacttcgga caataataaa
     6001 tgccggctat agcgccccaa ttcctctggt tgcaacttgc attctgttgt gggcatggta
     6061 cacctctaat tggtcaatga ctgttcgtaa tgaacgctat ttgtaattga attgctcaat
     6121 tgcaacggca tgtaattcgt tatttgtgcc cattagtcta tggacataga aaatatcatc
     6181 tcgaagaatg tataccttgg tgtctacaag gccttcgtcg agtaaaaaat aaggtttttt
     6241 aagatcgaga tggtaggatt gaaggggctt agaaatgcct gtcaccaatt tatcgaagtg
     6301 ctctcctcct gatattgcta atgcctcgcg actaactgaa caaaaagcgg ctgactctat
     6361 aaaggcccat tggtcagcaa agtccatggc gtagcgattc agcctgaacg gctcttgatg
     6421 cgtggcaatt agcatccctt cactttctat cttgctcatg ttctgcttta caaaccgttg
     6481 gggcattaag tcgctacgtt cttctggagt gaagagcgat tgaatatcag ggtttagatg
     6541 gccagaggtt gctgcggagc tggtgtgatc gccgatattc acaggcctga aatagatctt
     6601 aaagctggca agaggatgag agtccaaaaa aaaatctgag ttaccctgga ttagaatgcc
     6661 tttttttagc accgaagaat gcgtgcgaat atttataact tcaccatcat gaaaaaggca
     6721 cgggcctttg tattccaacg agcagcccgc gacaactaca ctagcactat attctgcaat
     6781 catcttttga atactaacaa cgtgctctcg catccaacct acccaagaca cagaccctaa
     6841 ttgtagtaac tctctgcagt gtacttgcag aggtgtaaaa taagtcggac aggccgcgac
     6901 ttgggtaagt gagatgcgca taaatcaacc acctgcaata gcggttaata cgtccaatcg
     6961 atcagagtcc ttaacagggc tacttaactg agccggatca acctgttttc cgtttaaaaa
     7021 taactgatgg acttttctta cttgacccgc gtcatcgaaa agtacgcccg ctgactgagg
     7081 gtagtcattg gcaagcttct taagtgcatc ctcaattgaa tctgcctcaa tgtgcaaatt
     7141 cttctgaaaa tttacaaatc ttagtaaagt gccggaaaac gccaaattca tataatcacc
     7201 tattcgaaac agagttcacc tgatgtggcg taaagatcgc ccactgggtt tgatgggtcg
     7261 gtgcaggcgt cgaaggtcgt gttaaaaggt ataattcctt ttttcaaacc tgaaaaaatg
     7321 cccgtgcctt tttcaaacga aattgtttca atgccttcaa tcagttttgg attgccctct
     7381 gcatcagggc atttagcgct ggtaacttca aattcatcac cgccagaact gaatgtaccc
     7441 agtcgatttt tggtgccaaa tacatgacct ccatggattt caccgtcact ctctccctcc
     7501 tcgtgagagc cagacgcctc agccccactt acaggaccac ttattgttaa tgtctttttc
     7561 tttccttctt tggggaccaa tataaattta tatgtgccaa taacatgtga ggcgtctaca
     7621 gaagactctt ccattgtcgc gcgtactact gtgtagcagc ctttcaactt cttataggcg
     7681 cttacctctt cctcgtttgc tgataccgaa atactaaggc tagagcagga aaggagcatg
     7741 gctgaaacgg cgatgatttt tttcataatt aacccttttt taaagttgat ggaaagattt
     7801 acgtgtagct aaacgagagg acaagcccaa taattctagg taagaaatat gtctgctaag
     7861 ttgcagggcg cccaagaata agttaaatgc gtgcacactt attgtgcgct tgaaaatatt
     7921 tacttcgtat tgattcgaaa cgtatctggg attagcatgc agggtcttgg tgcattactt
     7981 taagggcgta catatgttgg caattggcag cgttgcaaaa caattgggct gcagtataga
     8041 aaccctgcga tactacgaga aaataggctt ggtgtctccc gcccatcgaa gtgacggacg
     8101 gcacagattt tacagcgacc agcaagtcaa agagctcaga tttatcatga gcgcaaaaaa
     8161 gcttggtttt tcgctcgaag aaattgcatc tttaataaaa cttggcaaaa cctctaagga
     8221 ttgtaggccg gtcttggctc tagtcgacca gcaaatcaac ataattcaag agaaaatatc
     8281 acagctacag cggcttaatg tcgctatgca caccctcgct actgagtgta gaagctgttg
     8341 cgcatttgat ccctccgcaa atgcctgcac tataattgaa gcgttaaata cataatttgc
     8401 attcaattaa atgtggctgg caagtctttt aagcatttaa aaaacagttt tcaaaattat
     8461 gtaattggag tgttacattt aaatttcaaa ttaacgcgtt tatttgtaag ttcttgatgt
     8521 aaccatacaa ggggtttttt tgaatttgaa tagataaaaa cgaaattaat ttattcgaat
     8581 ttgtttgtgt gacgcgcatc acgtttttga ttgtttctcc ttgtacctga agttgcttca
     8641 ggttttagag tccccgggtt taacttgatg ccaatagggg ctgtttatgc aaaattcgaa
     8701 atctaaaggc ttattcgtta ttggagttgc ttgcgcggcc tgttgtctgg tgccacttgc
     8761 agcattgata ggtgcgagtt ctttaatatc gttgagtgcg tatttccttg atgcgctttg
     8821 gtgcgcaatt cctttggtgg tggtaggtat agcgttattt tggcgcaagc gacaaaagaa
     8881 taattgttgc gtatctgaca ctaaatgcgg tgctggcgta tgtaacaacg aactgcccaa
     8941 acaataatta gcagcttgtt gaaataccta ggcggcgacg ctctggcaaa tttggcgaaa
     9001 aagccgcagt ttacaggcag tatatgaggg atttttcgcc aaatttaacg ccgccaacgc
     9061 gcgcaaggta gtatttcaac aagctgtaag gggccaactt gcggcgtggg agttggtggc
     9121 gcccgaaaaa tggtccgtac caattttttc tttcttcttt aatgatgcgc ataatgcgca
     9181 cagttctttc gtccctctga aggctcgcgc cggttgtaat catcagggtt gtattagcca
     9241 tgtcccagtc tcggctgatt gcagcggtca aaccttagtt ggcctgctaa gctagtgaaa
     9301 tggtcgggcc ttgaaccttc gaggaaaaat gccaagaaaa tcacgtcgtt aaaagcctgt
     9361 gcccaatatc cggccaacaa gggccaactg tgttataact tagcgaaaaa taacagcata
     9421 agggtctgtt catggccaaa cgccgcgtac tgatagttga tgactccaaa acggcccagg
     9481 tacgcctgcg caaaatgctt gagcggttcg atgtagatgt cgataccgtc aattccgccg
     9541 aagaagccct ggggtacctt tcctatcgtc aacccagtgt gatctttttg gatcaccaca
     9601 tggagggcat ggacggctta gcggcattaa aaatcattaa agccaacccg ggcaccgcgc
     9661 tgatcccggt gattatgtac acctccgaac agggcgatgt gtacgtgggc caggcccgcg
     9721 ccttgggggc cttggatatt atttccaagg aagtgattaa gcaggtggat ttagaaaagc
     9781 tgatgggcag cttgaatatc tacctaatcg gtgccgagcc gccggaggcg gagcaagtgc
     9841 cccatgccag cactctggat atagtggcgg atttagagga gatgccgatt gctccggccg
     9901 ctgcgagcct ggatgagttg ccgcccgcca acgatttagc gcgggtgcaa ttgcagatag
     9961 gccgcttgtt tgagatccac atcgccaaag tgcgccagga gattgaagac agcaccaagt
    10021 ttttaatgcg ccgcctgtcc aaagaaattc aagagcgctc agccaaaccc tatgtgcgcc
    10081 cggttacccc ggtggccaac ccctccaacg agcccttgcc gccgattgaa gtcgagtcgg
    10141 aaaaagagcg cggtatttcc acctttattc tcgccctggt gcttttgggc gtggcgtttt
    10201 tgggctatca cctgtacatc aacaatgcac acctgcagtt aatcgcggaa aaatacgatg
    10261 tgctgcagca gcagaacgcc aagcaggaag ccttaatcaa ccgcctgttt gagcagagtg
    10321 tgcagggcaa agcggcgccg gaattggaca ccaacatgat gctggagacc ttaagctggg
    10381 cctccaatat gaataaccag gtggcctttg gtgagcaggc gttaaatgac cagcgcattt
    10441 atccggtggg cgagttggtg ggggtgttaa aagcggctaa ttttgccggt accgtgtatt
    10501 tggatattca cctgggtaac ttctgtgtgg tggaagaccc cagcgggcgg tacatcctgc
    10561 cggagccgga gagcaatatc gaagactgtg tgttcctggc ggataagcag ccccaggtgc
    10621 ccttagatag ccacatatcg gtgggctttt taaattatgt gcactcagca cctgtattga
    10681 ctgaagggcc tattgagctg gaatttgcca tgcagggcta cagccagcct aaggccagtt
    10741 acccgccgaa aacctcgggc accagcgctg gggaatggaa cgaggcggcg cgcaaaaata
    10801 accgagtgag tttttcattt gcccgcaaat aaaatctgcg agtaggtaaa ttcaactcaa
    10861 caaagcaccc tttgaggtgc ttttttttgt ggccgggttt tattgagcct gcgccgcgct
    10921 ctgccagaac tcctcctgcc tgggcagggt ttttaccatg gtattgatgg cggcagcggt
    10981 tttggtcaat tcctggtgca gtaattcggc agataagtgg gccaccttgg gggagttgtg
    11041 gcggggcctg atgcccatgc cttcaaaaat ggccagccaa ctgctggggc gaaataaatc
    11101 gtcgctgcgc tcatcaatta gtccttgggc ggtgaataaa tccatgcgct cgtggaggct
    11161 gtcgcttatg ggcaggcttt ggcagtagcg ccaaaaggcg ctgtcctggc gctggctttt
    11221 tacgtagtgc agctgcacaa aatcccgcac ctcagcaaag tctttattca tgcgcagatt
    11281 gtaggcccgc gccaggctgg ggtcgcactg cttgtggggt aaaaagcgca ggaaaaaatc
    11341 taaaccgcgg gtaattaaat gaatcgccgt ggcctccaaa ggctcaataa acccggcggc
    11401 aaggcccagg gccaggcagt tgtgctgcca gaacacctgc ctgtggccgg tggcgaaggg
    11461 cagtacgcgg gcctgctgct cattgggcgc aagattggcc gcctggcaca gctccacaag
    11521 ggccttgtca tcgctaataa aatcgctgga atacacataa cccatgcccg tgcgcgagcg
    11581 cagggggatg gcccagcgcc aaccggctaa ggaggctttg gcctgggtgt ataaatgggg
    11641 gcgggcttgg ctgggggcgc gggtggccag ggcgcggttg cagggtaaga agcgcgacca
    11701 atcgcaaaag ggcaccttta aaaactggct gaatagcagg ctgtgaaaac cgctgcaatc
    11761 gataaaaaaa tcggctttat gctcactgcc atcgtccagt atcagcgctt cgataaaccc
    11821 gttgtcctgc tgccgggcgc ttaccacccg cgcggcctgg gcgctaaaag ggcggctggc
    11881 gcagcgctgt ttaagggctg caaccaggtg ttcggcatcc acatgcagcg cataattggc
    11941 gccgccgatg ggggtgtgct gggccagttg gggcgggaag aagcgcgctt ctttggccat
    12001 tacctggcag ggagagaagt cctccagggg cgccaccggg ccgtgttcct gggctttaag
    12061 ccagcagggg taaaactcct ggcgcaaaat tttcgctcca gtgctgccaa aggggtggaa
    12121 gaagcgcgag tgtttaaccc gccaatccac aaactcgatg cccaatttat aggtggcatc
    12181 ggtctgctgc ataaacaggg tttcatccag ccccagggat ttaatcaggc ccacaaaggg
    12241 cggcacggtg gattcgccaa ggccaatggt gcctatgtcc ggcgattcaa ttaaatgaat
    12301 atgacactgt tcgccgattt cttggctgag aatggcggcg gccagccagc ctgcggtacc
    12361 accgccgaga atggtgatgg aggttagcgg ttgcgacata acagatcggc ctggatgaac
    12421 atggggtggc ttaccatcgg gagtggtccg ccagggagta gggttcgtct tgttcctgcc
    12481 agtgggtgaa atcccaaaaa acgctgcagg aggtaatcag ggcgcgcttg agtgcatcta
    12541 tggctttatg gcgggggaag ttgctgcgcc aatgcaaata caagggccgc tggggcgcag
    12601 gattcaccgg cagccaggct aaaaattggt tctgggccac cgccgcctgg gcggaggatt
    12661 ttggcagcag ggttacggct aaatccgccg ccgtcacctg ttttaatagc tcaatgccca
    12721 ccgtggccag agtaatcgag cgcagcccca actgctgcag ggacatgacc agggcgctgt
    12781 cggcggcggg aatttgtaaa cgctcggcaa tttcatccag gcttaagcaa gtggcggtgt
    12841 gaaagggatg ttgggcgggc aacagcgcca cataggtttc atcggcgagc tttagggaca
    12901 cagtatccgg cgcttcaata agctggcaat tgagcagtgt gtcgatctcg ccggtgcgca
    12961 ggttggccac tagggcgccc gcttccgctt cctgcagcac caggggcata tgcggtgcca
    13021 gccacttcag gtggccgagc acctggggca accactccgg cgccaggccc tgttgcacac
    13081 caagttttaa cggcccgtgc aaatgatcgc ccatctgctg ggctagatct ttgagggttt
    13141 gcacttggcc gagggcggcc tgggcgcact gcaaaagggg ttcggcttga ggcaataaac
    13201 gcacgccgag tttactgcgc tcaaataatt ccagccctaa agcggcttcg agttttttga
    13261 tgcccatact taaattgggc tggctaacgt ggcacagggc ggcggccttg ccaaaatgtt
    13321 gcgttttggc cagggcgata aaaaagcgca attcgttgag cgtcatgggc gggcaaaagg
    13381 cgtagtgtgc caagttccgc aggcaaaggc gagacgcggg tagacatttt gcctgcccgt
    13441 caaaaaatta attacaccgt gcgcaccaca aatactcagc ctgctgtatt aatccccggc
    13501 ggcccaattt acttggggaa ataagaattt tatctcagcg gcaaaaacgc ttttattaac
    13561 ccggcactaa atgccgccgg gttaataata aacggcctct cagtggctga tcatccaagg
    13621 cctagcggag ggaaacattt tttcgccctg agcggtatac agcagggtgc gggacttttt
    13681 attctggcag ccgcagcctt ttttatgctg atgatcatct tcccgccgcg ctttagtgga
    13741 aaattccggt gcgtgttggg ctttttcatt gagggcgtgg gcttttttaa cttcaggcgg
    13801 caagttaaat aatgccgggt ttaccataat cacccgtggc gattgtttat tgcaggtggg
    13861 gcattggttg ggcaggtggg cttcttccat ggtggctaat tcaaaaaata aaccgtgctc
    13921 ggcgcattta tattcgtaga ctggcatagg taaaaaactc ctattgaaaa cggctggcag
    13981 atattaaaaa ggggaatccg gcggctaaac taatacagcg agcaaaaacc gccgcgattc
    14041 ccaaaaccga ctatttatcc ttcgacagtg gaatatccac tgaaccatcc accattttct
    14101 tcggcccatc gccattgggt ttaatatcga aggcaaaaat atccgtcggc agccacaggg
    14161 tggcgcaggc gttgggtata tccaccacgc cgcttatatg cccctgcact ggcgcggtac
    14221 ctaaaatggc ataggcctga gccttggaat aaccgaattt ggtcatgtat tcaatggcat
    14281 ttaaacaggc ttggcggtag gcgatgtgta catccaaata gtgttgtttg cctttttcat
    14341 ccaccgaaat accttcaaaa attaaataat catcgtattt gggggtgatg g
//
LOCUS       DAOQGS010000028        13996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2016_length_13996_cov_4.418191, whole genome shotgun sequence.
ACCESSION   DAOQGS010000028 DAOQGS010000000
VERSION     DAOQGS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13996
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_13996_cov_4.418191"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKZ52_01940"
     CDS             complement(<1..274)
                     /locus_tag="PKZ52_01940"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820100.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNG58555.1"
                     /translation="MSERLTLTTPDDWHIHLRDVDVLSHTVADCARTFRRAIVMPNLV
                     PPVTNAEEALAYGSRIKAQVPAGVDFEPLMVLYLTDQTSPATIRAAK"
     gene            388..1566
                     /locus_tag="PKZ52_01945"
     CDS             388..1566
                     /locus_tag="PKZ52_01945"
                     /inference="COORDINATES: protein motif:HMM:NF037805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNG58556.1"
                     /translation="MLCPAPLPVINPCRSRFWRAAVYLLCLLTGFGAVAARAITYSNF
                     IENGGWSSESSLFACKLIHTIPYLGKAEFEKPAGEPMVFHLLANNPSLKTGQAALVAA
                     PPVWKSSSGKRNLGFVPVKERKQQVEVQGFKPERMLAELFDGQELIISRKPRYGGDET
                     TRVAITPIGFRAAYRDYTACLAALLPVNFEQIQRTSIYFGSDQFELTPSEQNKLDNIV
                     TYLKADPIAQEFFIDGHTDSVGSQPDNLALAQKRAETVSRYLIKHGMPKDAFWVRWHG
                     ERYPVASNKDRLGRAKNRRVTIRLETEKTQAEDKGDSAPGGDTRPAEPGAPAAPVTAE
                     LPAAEPTPAAPAAAPAQAPTAAAPPATAAKPADTQTGAPVAPSAQAQPAAAPSEPGKI
                     "
     gene            1705..2424
                     /locus_tag="PKZ52_01950"
     CDS             1705..2424
                     /locus_tag="PKZ52_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNG58557.1"
                     /translation="MGRTFENRKAAMAKTAGMKTKIYSKFGKEIYVCAKSGGVDPSAN
                     LSLRRLIEKAKRSQVPGHVIEKALDKAKGVGGEVYTTVRYEGFGPGNCMVLVDCLTDN
                     NNRTIGDVRTCFNKCKAKLGAPGAVGHLFDHRAIFAFKHNDEDAVLEALMNHDVDVAE
                     IENEDGIITVIAPHTEFYKAKTALTEAFGDINMEMEEMSFVPQTHTELAGEDVQMMEK
                     FIDMLNDCDDVQEVYHNAIMN"
     gene            complement(2682..4154)
                     /locus_tag="PKZ52_01955"
     CDS             complement(2682..4154)
                     /locus_tag="PKZ52_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58558.1"
                     /translation="MPSLRPTYGQSPFPIWSELSYFESDTLSLRHRAQQNEPQALLAF
                     YILASGLRNLADYENSQRRVAAFIEGLPSDLRADSDRYRQALRLHQLMHQHFFVAPDS
                     RQQGGYDEDQSAIAAIFSTGQFNCISSSMLYIVLARYLEFNTRAAVLPSHVFVEINLG
                     DKGKAEVETTSPAGFGVAHDEAFYQAAAAWFKERGLPPSSLQDYQNRQLISPLQLAAL
                     SMLTQHTNAAHMSRSDSVRLGEISAFLDPNNAIAQSRRLQFYSSEVETLSEHGAWYDL
                     LRLYATTLADLRQIQPLFPDHKAIQHSIYWLHASALFAYAQTGNLEGFKTQVNTLLSL
                     PNHNQERALFETTLLDSSLHLLEYLGRQKQFEEALLFASGLEEHVHKNRKWGRNIATL
                     YNLWAIDRWQQQDWPGVVDVLEEYFTQPYQDASDTTAKKNLANGYRNWLGIYLATGDW
                     TGAKSVIESCSSKKLAVFACGELTAQLKSAMGLKSNKNEL"
     gene            complement(4362..4982)
                     /locus_tag="PKZ52_01960"
     CDS             complement(4362..4982)
                     /locus_tag="PKZ52_01960"
                     /inference="COORDINATES: protein motif:HMM:NF027431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4823 domain-containing protein"
                     /protein_id="HNG58559.1"
                     /translation="MSIQRQNCLVYKAVLSLVLGMGLAACTPQYSRELTRETLTAAHI
                     SDNYSLKRENNWVLPQQSKLFLAYPDVSLLDAEQPITRTQYQLDEQIKIHFTRRFAGS
                     ISQREPASLAQTFKDAQQINADFLLVPQLLAISANPYAEQAKKPARNAWQLNFRLYDA
                     RSKKLIDTLKMDAQQGLLNRRKQEPAELFAAGINQAAQALAGDWGK"
     gene            5175..6023
                     /locus_tag="PKZ52_01965"
     CDS             5175..6023
                     /locus_tag="PKZ52_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNG58560.1"
                     /translation="MLDLSPFFILDPQRPHRWVSRAQCPNFRGGLFGGQLLAHGLSAI
                     ANTYPDKPVHMLHTQFLAAGDTQSPIEYKVLCLRRGRSFSHFQIHARQKRRLIATMIA
                     ACHQGETGFTHQEPAPPLKPPELSHAQVLPVFEIDNWLLGHGEETPLEFVHAQGARLD
                     QSIEEDGASSCWLRIRAPLTQAQQLPALAFISDLGILASALIPHPTHLFHHNLAPASL
                     NHTLWLHSPQVDLNQWHLLTVTSPWAGHGRGLGQGYLYTAQGELIATATQEGLIRPVA
                     PPAKPV"
     gene            6067..6402
                     /locus_tag="PKZ52_01970"
     CDS             6067..6402
                     /locus_tag="PKZ52_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58561.1"
                     /translation="MERVTIADALRELEDLAGALDNAYWDTAQIQQKDVFYNLISMLH
                     SELNELAKLSVSDHYMAYEPITAPWRNATGKLRHLHSNLDGWIIRTKTATVLEQQLPQ
                     VIRMLTPPL"
     gene            complement(6479..7300)
                     /locus_tag="PKZ52_01975"
     CDS             complement(6479..7300)
                     /locus_tag="PKZ52_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58562.1"
                     /translation="MSVQKLIICVKWGEKFGPEYVNKLYGMARRHITGDFRLVCFTDN
                     PVGLRPEVESRPLPELGCPHPERTIGKWRKVVLWGEDVGGLSGPALFIDLDSVIIDNI
                     DGYFTHGSPDDVILARNWAKPFSRMGQTSIFRFPIGKNPQILANFRRDPQGTADKYHF
                     EQHYITSSVPGGIKFWPEDWTRHFRIHCLPYFPLRLFFAAPKPRKAKVVTFPGGPNPD
                     DVVIGRWDEGYPQFPTPWQHIKNGLAQPGGFKAKWRYIRHYVKRCPWIEEAWAEQ"
     gene            7479..8831
                     /locus_tag="PKZ52_01980"
     CDS             7479..8831
                     /locus_tag="PKZ52_01980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNG58563.1"
                     /translation="MRVAVIGVGYVGLVQAAVLADVGNQVTCVDINQARIDNLRRGIV
                     GIYEPDLDALVLKNVQDQHLFFTTDITQALKDCDIIFIAVGTPPGEDGSADLRHVLAV
                     ADSIGNFITEPKIIVNKSTVPVGTAHQVQQHILKALAARNISLDIEVVSNPEFLKEGC
                     AVADCMRPERIIIGTDNPQGLACELLQELYAPFNRNHDKLIVMDVRSAELTKYAANCM
                     LATKISFMNEMANLAELVGADIEMVRKGIGSDSRIGYQFIYPGCGYGGSCFPKDVKAL
                     IHTGEQQNYPLHLLQAVDSVNERQKTKLFNYLHLAFKGELKGKTIALWGLAFKPNTDD
                     MREASSLVVIKQLMAAGAQVQAYDPKAMEEAQKHIGANTAVRLLGTKEACLQGADALV
                     ICTEWQQFRSPDFEQIKHLLSAPVIVDGRNLYDPARMKKRGFVYYAIGRGESVQSIRD
                     "
     gene            complement(9142..9468)
                     /locus_tag="PKZ52_01985"
     CDS             complement(9142..9468)
                     /locus_tag="PKZ52_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58564.1"
                     /translation="MQNINLGRFLRVDVSGGQDITVQLLPDYSLEEEVLCCWDGDKLV
                     CAIGADEQPGGYSDQEYWGGMLTALQADPDNTVSVIKEGEYHNQDGSLLNYKIFKLQM
                     GEVILH"
     gene            complement(9575..10051)
                     /gene="comJ"
                     /locus_tag="PKZ52_01990"
     CDS             complement(9575..10051)
                     /gene="comJ"
                     /locus_tag="PKZ52_01990"
                     /inference="COORDINATES: protein motif:HMM:NF022486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComJ"
                     /protein_id="HNG58565.1"
                     /translation="MMAKLVIQIYLSYSQLCVFLTSLTNPFNDWSDRSFSQGFSWRDG
                     SVSFRALREDGDHQINLFIDEPIPGLVSNVQRAFRVPFETMDGHIEVASISDSACLEV
                     PPGKYCLQVEFLAVEDNGMPEVNVRLNKGGCGFELLKADDQIVMEGELDISAESAI"
     gene            complement(10048..>11319)
                     /locus_tag="PKZ52_01995"
     CDS             complement(10048..>11319)
                     /locus_tag="PKZ52_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNG58566.1"
                     /translation="SGKLMFSQNFNGCNVSRTAHIYLGNEAVAERTTRGHVEYMDLNQ
                     NGIDDCTDEKSGTNSAFNFDAPNLWQGLTQPEGPLIKLSRHQSHKQADAPLSAGMGWL
                     FLLPFLANYLARGRRFFKQVFFACFMAASGNALAAVTEEITYLHTDLSGSVKAASDKN
                     GNLLWRQEYTPSGAATLPTGQKIGFNNFQQDASGLVYMGARYYDPHIQRFLSPDPASQ
                     VTSVESNPMMVNRYAYANNNPVSYLDPDGNNPKLVLDFALNVGINMLVTGQPGFASAA
                     VETAKGAFNPLATVSKVKKLAEAVRAANKVMAERAVVGKVAKDIPISRAKYGEAADHI
                     ADAQKAGHPDVLEIARDGASANRGASIGGLSKVPGKQLDEYPPAMFKEGGAGASVRAI
                     SPKDNMAAGACIGNACRGLANGEKVRIKVVP"
     assembly_gap    11320..11419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11420..13970)
                     /locus_tag="PKZ52_02000"
     CDS             complement(<11420..13970)
                     /locus_tag="PKZ52_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58567.1"
                     /translation="MYDNPYVAAVKTKTVIDPITNKTGTWTYVFNQQAMYGQARDRGT
                     GAEFLIYLDRLTVEPPAGYPKKLYYHKSAYPYQGRRSGGQSIGKKTMEATFEISQNYL
                     VEAKTYQDGDGERIISDENFHLVGTSEYDDFTYAYSEPGEIVSRDKIEFIPLNGQMVD
                     RIRASHYSGAVEFDEFGQPTKVMESVRGAPSNGQFQLVPGRSLSNSILDPFTIVATTN
                     YYKYYSDKWLLGKVDYSYRSEVERGDGGLNELKFLNYSLTLFNDFGDLFFKYNSADGS
                     RFDYEYQPDTRLLARKSSPSGFDESYGNFRAGIPQSLTKQVGAGQSPISETSVVDFWG
                     QVGAFTNTHGYTKSYEYDDLGFIKKITPPRGNVTDVVRMGNRVTITRGNLTQVEDLDG
                     FGRVIAKTISGDGSSIKTTYEYDLLGQQVFASLPNSTQGKTTQYDGLGRVKRTEEPGY
                     VVENYYRDVDVLTNETIEGRDGVVQTLRRYSMLGTDYDDAKLVDVITVFDSYDPRDPV
                     GYYIAESTGYQRDLDGRLLAVLPMKIDSDGGRVSLGSLDKNPTFTYDWRGQLETTFNP
                     EEGLRYFSYDSDKPNNTNVTGIFDSNPYQNPDARKLFAMEYDNLGRMTKQTDLVTNLV
                     TDWFYDMDRLDGTSAGLSYENEDARVRKSYFYNENNYLTREITMPGAAFWNGPLLNPY
                     SLVYGYDNNDHMSFVRYPGNLQVNYKPDAFGRPTQALPYVTNVTYHPHGLIDTITYAN
                     GVVKSVGYDDQRQLPNSLSVKKANYLINSSQTLYGDGTIHTRSDNAQAGIHAGDVGGT
                     YTYRYDAKKRLRIATHDWGSNRLSYVYNFDERERIFMSPAGDVFDYDDQNRL"
BASE COUNT         3565 a         3769 c         3282 g         3280 t
ORIGIN      
        1 gcttggcggc gcgaatggtg gcggggctgg tttgatcggt caggtacagc accatcaagg
       61 gctcgaaatc cacaccggca ggcacctggg ccttgatgcg ggagccgtag gccagggcct
      121 cctcggcgtt ggtgaccggt ggcaccaggt tgggcataac aatcgcgcgg cgaaaggtgc
      181 gggcacaatc ggccacggta tggctgagca catccacatc gcgcaggtga atatgccaat
      241 catctggggt ggtgagagta aggcgctcgc tcataacagg ttccaacaac aaaatccgcc
      301 gcatgctacc ggaattaata cctaaagccc aggcgcttag ggccgataac ccgaacgaag
      361 cctaagactt aaattcgtgg aggccctttg ctgtgccctg cccctttacc tgtcattaac
      421 ccctgccgca gccgcttttg gcgcgccgcg gtgtacttgc tatgcctgct cacgggcttt
      481 ggggcggtgg cggcgcgggc cattacctac tcgaacttta tcgagaacgg cggctggagc
      541 agcgagtcgt ccctattcgc ctgcaaacta atccacacca ttccctacct tggcaaagcc
      601 gagttcgaaa aacccgctgg ggagcccatg gtatttcacc ttttggccaa caaccccagt
      661 ttaaaaaccg gccaagcggc actagtggcg gcgccgccag tgtggaaatc cagcagcggc
      721 aaacgcaatt tgggctttgt gccggtaaag gagcgcaaac aacaggtgga ggtacaaggc
      781 tttaaaccgg agcgtatgct ggcggaatta ttcgacggcc aggagttgat tatcagccgc
      841 aagccccgct acggcggcga tgaaaccacc cgcgtcgcca tcacccccat aggttttcgc
      901 gccgcctacc gggattacac cgcctgctta gccgccctat tgccggtcaa ttttgagcag
      961 atccagcgca cctccattta tttcggctcc gaccaatttg aactcacccc cagtgagcaa
     1021 aataaactcg acaacatagt gacctactta aaggccgacc ctattgccca ggaatttttt
     1081 atcgacggcc ataccgattc ggtgggcagc caaccggaca acctggccct ggcgcaaaaa
     1141 cgcgcggaaa ccgtgagccg ctacctcatc aaacacggca tgcccaaaga cgccttttgg
     1201 gtgcgctggc acggcgagcg ctacccggtg gctagcaata aagaccgcct agggcgggcg
     1261 aaaaaccgcc gggtgaccat ccgcctggaa acagaaaaaa cccaagcgga agataagggc
     1321 gattcggccc ccggtggcga cacccgcccg gctgaacccg gcgccccggc cgcccccgtt
     1381 acagcggaac tgcccgccgc agaacccact ccagctgccc ccgccgccgc accggcgcaa
     1441 gcacctacag cggctgcgcc acccgctacg gcggcaaaac ccgccgatac gcaaacgggc
     1501 gcaccggtag cgcccagcgc ccaagcacag ccagcagctg cacccagcga gcccggcaaa
     1561 atataagcgg cataccgatt cacctagaaa ccgcagcagg gcttaactgc ggtttctagg
     1621 tttaagacta gggcttaacg atagagacct gagcaggcat aattgccgca attttttgtc
     1681 ctactcttac agtcgagcat agccatggga agaaccttcg agaaccgcaa agccgcaatg
     1741 gcaaaaactg cgggcatgaa aaccaagatt tactctaaat tcggtaaaga aatctacgtc
     1801 tgcgccaaaa gcggcggcgt ggaccccagc gcgaacttaa gcctgcgccg cttaattgaa
     1861 aaagccaaac gctcccaggt gcccggccac gtgattgaaa aagccttgga taaggccaag
     1921 ggtgtaggcg gtgaggtgta taccaccgtg cgctacgaag gctttggccc cggcaactgc
     1981 atggtgttgg tggactgttt aaccgacaac aacaaccgca ccatcggcga tgtgcgcacc
     2041 tgttttaata aatgcaaagc caaactcggc gcccccggtg cggtgggcca tttattcgat
     2101 caccgggcga tttttgcctt taaacacaac gatgaagacg ccgtacttga agcgcttatg
     2161 aaccacgatg tggacgtggc cgaaattgaa aatgaagacg gcatcatcac ggttatcgcc
     2221 ccgcacacgg aattttacaa ggctaaaacc gcactcaccg aagccttcgg cgatatcaat
     2281 atggaaatgg aagaaatgtc gtttgtgccg caaacccaca cagaattagc cggtgaagat
     2341 gtgcaaatga tggaaaaatt catcgatatg ttaaacgact gcgatgatgt gcaagaggtt
     2401 taccacaacg ccatcatgaa ttaatggcct gcccgcgcac taaaaaagcg gctgtttagc
     2461 cgctttttta gtgcctgaag gattcccctt tggcacaact attcggatca aagatacaag
     2521 gttgggtaga gcctagcgaa acccaacaca acagcacata aacatggcca gaaaatatcg
     2581 ggcgctgttt gatttattcc aacgtaaaac cattttctcc gttattagaa gttgggttta
     2641 gcaagctcca cccaacctta ctacttgcca cctgctttta atcacaactc atttttattg
     2701 ctttttaaac ccatcgcact tttcagctgc gccgttaatt ccccacaggc aaacaccgct
     2761 aattttttac tgctgcaact ttcaatgaca cttttagccc ccgtccaatc ccccgttgcc
     2821 aaataaatac ccaaccagtt gcgatagcca ttggcgagat ttttctttgc cgtagtatca
     2881 ctagcatctt ggtagggctg ggtgaaatat tcctcgagca cgtccaccac accgggccaa
     2941 tcctgttgtt gccagcggtc gatggcccag aggttataca gcgtggcgat attgcggccc
     3001 catttacggt ttttatgcac atgctcttca agaccgctgg caaataatag cgcctcttca
     3061 aattgttttt ggcggcctag gtattccaat aagtgcaggc ttgaatccaa taaggtggtt
     3121 tcaaataatg cgcgctcttg gttgtggttg ggcaagctca atagagtatt aacctgggtt
     3181 ttaaaaccct ctagattacc ggtttgagca taggcaaata acgcactggc atgcagccaa
     3241 taaatgctgt gctggatggc tttgtggtcg ggaaatagcg gttgaatttg ccgtaaatcc
     3301 gccagcgtgg tcgcgtacaa acgcagcaaa tcgtaccagg cgccatgctc acttagggtt
     3361 tctacttcac tgctgtaaaa ctgcaagcgg cgggattgcg caatggcatt attggggtct
     3421 aaaaaagcgc taatttcccc taggcgcaca ctgtcactgc ggctcatatg ggcggcgttg
     3481 gtgtgttgag tcagcatgct taaagcagct aattgcagtg gtgaaattaa ttgccggttt
     3541 tgataatcct gcaggctaga cggcggcagg ccccgctctt taaaccaggc tgcggcggct
     3601 tgataaaagg cttcatcgtg cgcaacccca aagcctgcgg gggaggtggt ttcaacctcg
     3661 gctttgccct tatcgcccaa attaatttcc acaaatacgt gggagggcaa aacggcggcg
     3721 cgggtattaa attctaaata gcgcgcgagc actatgtaca acatgctgga gctgatgcag
     3781 ttaaattggc cggtggaaaa aatagcggca atggcggatt ggtcttcatc gtaaccgccc
     3841 tgctggcgag aatccggcgc cacaaaaaaa tgctggtgca ttaattgatg caagcgcagg
     3901 gcttggcggt agcggtcgct gtcggcgcgc agatcggacg gcagcccctc aataaacgcc
     3961 gcaacccgcc gctggctgtt ttcgtaatcg gctaaattgc gcaggcctga ggcgagaata
     4021 taaaacgcca acaatgcctg gggctcattc tgctgagcgc gatggcgcag gctgagggtg
     4081 tcgctttcaa agtaggagag ctctgaccaa atgggaaatg gcgattggcc gtaggtgggc
     4141 cgaaggcttg gcacctctgc ccgagcgggg ctcaccgcga gatgaaaaac aacaagaaga
     4201 taaaaaagcc cgcaggcaat tcgctttaac atacacacgc acaacaggag ccaaccggtt
     4261 ttcgggccaa tttacgctgg gttagttccc agcccagggc gactctaacg caatttctcc
     4321 tgcacagctg ccgcagcagc cacaaattgc caagcaacag cctatttgcc ccaatcaccc
     4381 gctagggcct gggcggcttg gttgatacct gcggcgaata attcggcggg ctcctgcttg
     4441 cggcggttta acaacccctg ctgggcgtcc atttttaagg tatcgatgag ttttttgctg
     4501 cgcgcatcat agagacgaaa attcaattgc caggcattgc gcgcgggttt ttttgcctgt
     4561 tcggcatagg gattagcgga gattgccagc agctggggca ccaataaaaa atccgcgttt
     4621 atctgctggg cgtctttaaa ggtctgcgcc aggcttgccg gttcgcgctg gctgatactg
     4681 ccggcaaaac ggcgggtaaa gtgaatttta atctgctcgt ccaattgata ttgggtgcgg
     4741 gtgatgggtt gttcggcgtc caacaggctg acatcgggat aggctaaaaa cagtttgctc
     4801 tgctgcggca atacccaatt gttctcgcgc tttaggctgt aattatcgct gatgtgcgca
     4861 gccgtcaaag tctcgcgggt taactcgcgg ctgtactgag gcgtgcatgc tgccaacccc
     4921 ataccgagca caaggctcag cacagcctta taaactaggc aattttggcg ctggatactc
     4981 atatgaatcc ccttacctgc tgagcggaaa ccgcctgcgc ttgctataac acagcctatg
     5041 ggccacattt gctagggtgt gaagtattta tccgggcctg tggccacagg tataaacacc
     5101 ccctcgcgcc caaagtatag gtgcccctcc ggcgcccgta agcccaatct cgccccctta
     5161 gcgaatttgc ccctatgctc gatttatcgc cattttttat tctcgacccg cagcgccccc
     5221 accgctgggt aagccgggcc cagtgcccga attttcgcgg cggtttattc ggcggccaat
     5281 tgctggccca cgggctgagc gcaatagcca atacctaccc cgacaaaccc gtacacatgc
     5341 tgcacaccca attcttagcc gccggggata cccaaagccc catcgaatac aaagtcttgt
     5401 gtttgcgacg ggggcgctcg ttcagccatt ttcaaattca cgcccggcaa aaacgtcgct
     5461 taattgccac catgatcgcc gcctgccacc agggggaaac gggttttacc caccaggaac
     5521 ccgccccgcc ccttaagccg cctgaattga gccacgccca ggtattgccc gtgtttgaaa
     5581 ttgataattg gctgctcggg cacggtgaag aaactccctt ggagtttgtc catgcccagg
     5641 gggcgcggtt ggatcagagt atcgaagagg atggcgcgtc cagctgctgg ctacgtattc
     5701 gcgccccttt aacacaggcc cagcaattac ctgccctggc gtttatctcc gacctgggca
     5761 tcctcgccag cgcccttatc ccccacccga cccatttgtt ccaccacaac ctggcccccg
     5821 ccagcctcaa ccacaccctg tggctgcaca gccctcaggt ggatttaaac cagtggcacc
     5881 tgttaaccgt gaccagcccc tgggctggcc acggccgggg ccttggccag ggctatttgt
     5941 acaccgcgca gggggaatta atagccacgg ccactcaaga gggattaatc cgccctgtcg
     6001 cgcccccggc aaagcctgta tgagcgacta tacttgtgaa acataaccta attgatgagg
     6061 taaccaatgg agcgcgtgac cattgcagac gctttgcgtg aattggaaga cttagccggt
     6121 gcgcttgata acgcctattg ggacactgcg caaattcaac agaaggatgt tttttataat
     6181 ttgattagca tgctgcacag cgaacttaac gagctggcga agctgagcgt atcggaccat
     6241 tacatggcct acgaacccat cactgcgccc tggcgcaatg ccaccggcaa gcttaggcac
     6301 ctgcacagca atttagatgg ctggattatt cgcaccaaaa ccgcaaccgt gttggagcag
     6361 caattacccc aggtgattcg catgttaacg ccgccgctgt aatttattta caacgccata
     6421 aaaaaagcca cgcagtcgcg tggctttttt tatggcgcgg tgtgtagttg tgtgctggtt
     6481 actgctccgc ccaagcctct tcaatccagg ggcagcgctt cacatagtgg cggatatagc
     6541 gccacttagc cttaaaacca cccggctgcg ccaagccatt tttaatatgc tgccaagggg
     6601 tgggaaattg cggatagccc tcatcccaac ggccgatcac cacatcatcc gggttgggcc
     6661 caccggggaa agtcaccacc ttggctttgc gcggcttagg cgcggcgaag aataggcgca
     6721 gcgggaaata gggcaggcag tgaatgcgaa aatgccgcgt ccaatcctcc ggccagaact
     6781 taataccgcc gggcacagaa gacgtaatgt agtgctgctc gaagtgatac ttatccgccg
     6841 tgccctgcgg gtcccggcgg aaattggcga ggatctgcgg attcttgcca atggggaagc
     6901 ggaaaataga cgtctgcccc atgcgggaaa acggcttggc ccagttgcgc gccaggatca
     6961 catcatccgg tgagccgtgg gtgaaatagc cgtcgatgtt atcgataatc accgagtcca
     7021 aatcaataaa cagggcaggc ccactcaagc cgccgacgtc ctccccccac aacaccacct
     7081 tgcgccactt gccgatggtg cgctccgggt gggggcagcc caattccggc agcgggcggg
     7141 actcaacctc cggccgcagg cccacggggt tatcggtaaa acacaccagg cgaaaatccc
     7201 cggtgatatg cctgcgcgcc atgccataga gcttattgac atactcaggg ccaaattttt
     7261 cgccccattt cacgcagata attaattttt gaacgctcat gtagatatac ttttttgggg
     7321 cggcagctaa gagcaccacc taaaaataaa aggccctaca ttacaggccc gaaaaggcaa
     7381 agggcgcgat tctatacaag cccccccgca aaagcaaaag cccagcaggc caggttttac
     7441 ggtagtattg gcgcttttta agcagcggaa atctttacat gcgcgtagca gtgatcggtg
     7501 tggggtacgt gggtttggtg caggcggcgg tgcttgccga tgtgggcaat caagtgacct
     7561 gtgtggatat caaccaagcg cgcatcgaca acctgcgccg gggcatagtg ggcatctacg
     7621 agccagacct agacgcgcta gtgcttaaaa acgtgcagga tcagcacctg tttttcacca
     7681 ccgatatcac ccaagccctc aaagactgcg acattatttt tatcgccgtg ggcaccccgc
     7741 cgggggaaga tggctcggcg gatttacgcc atgtattagc ggtggccgat tccatcggca
     7801 attttattac cgagccgaaa attatcgtca ataaatccac cgtgcccgta ggtaccgccc
     7861 accaagtaca acaacatatt ttaaaggccc tcgccgcgcg caacatcagc cttgatattg
     7921 aagtggtttc caacccggag tttttaaaag aaggctgcgc tgtggcggac tgcatgcggc
     7981 ctgagcgcat tattatcggc accgataacc ctcagggcct ggcctgcgaa ttattacaag
     8041 agctctacgc gccgtttaac cgcaaccacg ataaattaat tgtgatggac gtgcgctcag
     8101 cggaactcac caaatacgcc gccaactgca tgctggccac caaaattagt tttatgaatg
     8161 aaatggccaa tttggcggaa ttggtcggcg ccgatattga aatggtgcgc aaaggcatag
     8221 gctcggattc gcgcatcggc tatcaattta tttaccccgg ctgcggctac ggcggctcct
     8281 gttttcccaa agacgttaaa gcattaattc acaccggcga gcaacaaaac tatccgctgc
     8341 atttattgca agcggtggat agcgttaacg agcggcagaa aacaaaatta tttaattatt
     8401 tacacttggc atttaaaggc gaacttaaag gcaaaaccat cgccctttgg ggcctagcct
     8461 tcaaacccaa taccgatgac atgcgcgaag cttccagcct ggtggtgatt aaacaattaa
     8521 tggccgctgg tgcccaggta caggcctatg accccaaggc tatggaagaa gcgcaaaaac
     8581 acatcggcgc taacaccgcc gtgcgcttgc tgggcactaa agaagcctgc cttcaagggg
     8641 cggatgccct agttatttgc accgaatggc aacaatttcg ttcaccggat tttgagcaga
     8701 taaaacactt attatcagcc cccgttattg ttgacgggcg caacctgtac gatcctgccc
     8761 gcatgaaaaa acgcgggttt gtttattatg cgattgggcg gggtgaatca gtgcagagca
     8821 tccgcgacta aaattggctc tgtgcgtagg ttggaagggc gcagcgactt ccgacatttt
     8881 taggcgagca catgcacagt ttaagatcgc agtttagagg agatattcac atcgaagggc
     8941 gttgcactgt tccgaccgac tacaaaccca agaaacccca ggttaagagc tttaacactc
     9001 agacaaaaga ttggccagtt caattctggc cgagaaaaga atctcaccca ttagccgatt
     9061 aacttgaaaa aagtcttcgc aatttatact cacggtaaaa atccaatagg caacctgttg
     9121 attatgaatt aaactcataa tctagtgcaa aatcacctcc cccatttgca atttgaaaat
     9181 tttatagtta aggaggctgc catcttgatt gtgatactca ccctctttaa ttactgaaac
     9241 cgtgttatct gggtcagctt gaagcgcagt caacatacct ccccaatatt cttgatcaga
     9301 atagccgccc ggttgctcat cggctcctat agcacaaacc aatttatccc catcccaaca
     9361 acaaagaact tcttcctcca aggaatagtc aggcaacaac tgcacagtaa tatcttgccc
     9421 gcctgacaca tcaacacgta aaaaccgacc tagatttatg ttctgcataa aatccccatc
     9481 ttaaacatcc tcgccacaat caccttagtt tcaaatattg ggcatcaaaa ctttacaagc
     9541 taaacatcta aatgtttttc tctaacaccc aagctcagat tgcggattct gcactaatat
     9601 ccagctctcc ttccatcact atttgatcgt cagccttcaa taattcaaag ccacaaccac
     9661 ccttatttaa acgaacattt acctccggca ttccattatc ttcaaccgcc aaaaactcaa
     9721 cttgcagaca atatttgcca ggcggaactt ccaagcatgc agaatcagaa atgctcgcaa
     9781 cctctatatg accatccata gtttcaaatg gcactctgaa agctcgttgc acatttgaaa
     9841 caaggccagg aataggctca tcaataaata gattaatttg atgatcccca tcctcgcgaa
     9901 gcgccctaaa tgaaacgctt ccatccctcc atgaaaagcc ttgtgagaaa cttcggtcac
     9961 tccaatcgtt aaatggatta gttagggaag ttagaaacac acaaagctga gagtaagata
    10021 agtaaatttg aataaccaac ttagccatca tggaaccact tttatccgaa ctttctcgcc
    10081 atttgcaagc cctcggcatg cgttaccgat acacgcacct gccgccatat tgtctttggg
    10141 gcttattgcc ctaacactcg cacccgcgcc accttcttta aacatcgctg gagggtactc
    10201 atcaagctgt ttacctggta cttttgaaag cccgccaatt gatgcacctc tatttgctga
    10261 tgccccatca cgagcaattt ccaacacatc tggatgaccc gctttctgag catcggcgat
    10321 atgatccgca gcttcaccat attttgccct ggaaattggt atatccttcg caactttgcc
    10381 caccacagcc cgctcagcca taactttatt agctgcccga accgcctcag ctaatttttt
    10441 aactttgctg acagtagcca gtggattaaa cgccccctta gccgtttcca ccgctgcact
    10501 ggcaaaccca ggctgtccag tgaccagcat attaatgccc acattcaacg caaaatccaa
    10561 aaccaatttt gggttattgc cgtctgggtc taaataactt acgggattat tgttggcata
    10621 ggcgtatcgg ttcaccatca tcgggttaga ctcgacactg gttacctggc tagctgggtc
    10681 tggtgacaaa aagcgttgga tatgcgggtc gtaataacgt gcgcccatat acacaaggcc
    10741 actagcgtcc tgctggaaat tgttaaagcc tattttctgc cctgttggca gtgtggcagc
    10801 gccagacggt gtgtactcct ggcgccacag taaattgcca tttttgtccg atgcagcttt
    10861 gacgctgcca ctcaaatccg tatgcaggta ggttatttct tcagtaacag ctgccaaggc
    10921 attgccactt gccgccatga aacaggcgaa gaacacttgt ttaaagaagc ggcggccgcg
    10981 cgccaaataa ttagctagga atgggagcaa aaacaaccag cccattccgg cagacagagg
    11041 cgcatcagct tgtttatggg attggtgccg tgaaagtttt attaatggcc cttcaggctg
    11101 ggttaaaccc tgccacaaat taggggcatc gaaattaaat gcgctattag tgcccgattt
    11161 ttcatcggtg cagtcatcta tgccattttg atttaaatcc atatattcaa cgtgtccgcg
    11221 cgtagtacgc tccgctacag cctcattacc caaatatata tgcgctgtac gagagacgtt
    11281 acagccgttg aaattttgcg aaaacatcaa cttacccgan nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnt taaccgattt tgatcatcat agtcaaagac atccccagca
    11461 ggtgacataa aaatgcgctc acgctcatca aaattataaa catagctcag acggttacta
    11521 ccccaatcgt gcgtagcaat tctcaggcgc ttcttagcat catatcgata ggtgtatgta
    11581 ccccctacat ctccagcatg aatgccagct tgggcattgt ctgaacgtgt atgtatggtt
    11641 ccatcgccat agagtgtttg gctgctgtta attaaatagt ttgccttttt aacactcaga
    11701 ctattgggta actggcgttg atcatcataa ccaacacttt ttacaactcc atttgcataa
    11761 gtaattgtgt caatcaaacc gtgggggtgg taggtgacgt ttgttacata aggcaacgcc
    11821 tgtgtagggc gaccgaacgc atccggctta tagttgacct gcaaattccc cgggtacctc
    11881 acaaagctca tatgatcatt attgtcatag ccgtaaacca aggaataagg attcagcaaa
    11941 gggccgttcc agaaggcagc acccggcatc gttatctctc ttgtaagata gttattttca
    12001 ttgtaaaaat aagacttacg tacccgagcg tcctcatttt cataacttaa tcccgcactt
    12061 gttccatcca atcgatccat atcataaaac caatcggtca ctaaatttgt aaccaaatca
    12121 gtttgtttgg tcatgcgacc taaattgtcg tattccatgg caaataactt gcgcgcatcg
    12181 gggttttgat aaggatttga atcaaagatc ccggttacat tggtattgtt gggcttatcg
    12241 ctatcatagg agaaataccg aagcccctcc tcgggattaa atgttgtctc caactgccct
    12301 cgccagtcat atgtaaacgt aggattctta tccaaagatc ccaaagaaac gcgcccacca
    12361 tctgagtcaa ttttcatagg caaaacggca agtaaacgcc catcaagatc acgctgatat
    12421 cccgtcgatt cagctatgta gtaacctact ggatcacgcg ggtcgtagct gtcaaaaaca
    12481 gtgataacat caaccaattt tgcatcgtcg taatccgtac ccagcatcga atagcgtctt
    12541 agggtttgca cgacaccatc tcggccttcg attgtttcat tggttaaaac atcaacatcc
    12601 cggtaataat tttcaaccac atacccaggc tcttcagttc gttttacccg accgagacca
    12661 tcgtattgag tagttttacc ctgggttgaa tttggcagcg atgcaaaaac ttgctgcccc
    12721 agcagatcat actcataggt ggtttttatg gagctaccat cgccggagat tgtttttgca
    12781 atgacccgtc caaaaccatc cagatcctcc acctgagtca aattacccct cgtgatagtg
    12841 actctattcc ccatgcgcac aacgtcggtc acgtttcctc tgggaggtgt gatttttttg
    12901 ataaagccta aatcgtcgta ttcataactt ttcgtgtagc catgggtatt ggtgaaggct
    12961 cccacctgcc cccaaaaatc tacaacacta gtttcactaa tcggagattg tcccgcaccg
    13021 acttgttttg ttaatgactg gggaatccct gctcgaaaat taccgtaaga ctcatcaaat
    13081 ccagaggggc tagacttgcg cgcaagcaaa cgagtatcag gctgatattc gtaatcaaat
    13141 ctcgacccat ctgccgaatt atatttaaaa aagagatctc caaagtcatt gaataatgtc
    13201 aggctataat ttaaaaactt cagttcattc agtccgccgt cgcctctctc tacctcgctt
    13261 cgataagagt aatccacctt tccaagcaac catttgtcag aataatattt gtagtaattc
    13321 gtcgtcgcga caattgtgaa tggatctaaa atactgttcg acagagaacg ccccggcacc
    13381 aactggaact gcccatttga tggcgctcct ctaacacttt ccatcacctt agtcggttga
    13441 ccgaactcat caaactctac ggcgccgcta tagtgagagg ccctaattct gtccaccatt
    13501 tgcccgttta gcggaatgaa ttcaatctta tctctagaaa caatttcgcc tggttcacta
    13561 tatgcgtagg taaaatcatc atattcactt gtgccaacca aatgaaaatt ctcatccgaa
    13621 ataattctct ccccatctcc atcctgatag gtcttagctt caacaagata attttgcgaa
    13681 atctcaaagg tggcctccat tgtttttttg ccaatacttt ggccaccaga tcgccttccc
    13741 tgatagggat aggctgactt atggtaatag agtttcttcg ggtatcctgc gggcggttca
    13801 accgtcagac gatccagata gataagaaat tcagcgccag tgcctcgatc tctagcttgt
    13861 ccatacatag cctgctgatt aaatacatag gtccatgtgc ccgttttatt tgtaatcgga
    13921 tcaattacag ttttcgtttt cacagctgca acataggggt tgtcatacaa agtaaaactt
    13981 gggtgcagcc ttacgc
//
LOCUS       DAOQGS010000029        13973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2021_length_13973_cov_5.132562, whole genome shotgun sequence.
ACCESSION   DAOQGS010000029 DAOQGS010000000
VERSION     DAOQGS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13973
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_13973_cov_5.132562"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1349)
                     /locus_tag="PKZ52_02005"
     CDS             complement(69..1349)
                     /locus_tag="PKZ52_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG58568.1"
                     /translation="MHTNPIDETALYARVTWRLLPILFLCYVASYLDRVNVGFAKLQM
                     LNDLKFSETVYGLGAGIFFIGYFIFEVPSNIALHKVGARLWIARIMITWALVSAAMVW
                     VTTPLSFYILRFLLGAAEAGFFPGVILYLTYWFPAHRRGKITALFMTGVPISGVIGGP
                     LSGWIGASLPGVYGLAGWQWMFILEAIPSLLLGIVVIIVLADGIHSAHWLNDAEKTLL
                     DNNIRREEQSKSTLSARHMLADSKVWLMAGIYFCFVMGLYGVSFWLPTLIHNLGVENS
                     AEVGWLTAIPYAVASITMILLGHSADKHRERRWHLAVPALLGGAGLILSALWAHNTLL
                     AMAALSLATAGIISILPLFWSLPTAILGGVAAAAGIALINSLGNLAGFLSPYLVGWLK
                     DATASTDSGLFMLAGFLFLGAALTLTTPKQQVNA"
     gene            1567..1749
                     /locus_tag="PKZ52_02010"
     CDS             1567..1749
                     /locus_tag="PKZ52_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58569.1"
                     /translation="MARLFFMALCCALSACVAPEKDSNSQLWINEVVTDNDGVAIDEN
                     GQTSDFSSLLILAPRA"
     gene            1875..4514
                     /locus_tag="PKZ52_02015"
     CDS             1875..4514
                     /locus_tag="PKZ52_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNG58570.1"
                     /translation="MPFKLSAQGETLTLRNKLGESYQQVKIPALGINDSYSRFPSGKG
                     AFAICRYATPNRSNGTACQSVSHPGLTESVVFKPFDTAQWPSLTPPSLGINELALLPA
                     QFIEVKNFSQQFVDLTQFKLILTAYPPNAPLPGFNHHHSVPLPALSLAPGAIATIPID
                     GDQVQAIAQQAFNEGVAVLYDRNTQSPVDQVPFMHWPLNHALARQEHYPYRLRFCDNP
                     TPNQDGACAETQQRPIGNRSRGLYTPGDFAALAAGSGLNQIQSVKFVLDLQRQNAVHF
                     LGSREWPLHYTFVREIIDGDTRLNRCENEDYRLFMNGWARFSVENYFNNTTRRYHLGT
                     LSFYPNAQLANVEFTFGDEITAAQMRAAFYAVTAATANPFNWSLRPQDNPQVERARAI
                     EGTLPIVGPQAPFANLVFQGLAPGVAYGTLKIIPTTELTSASLGLRTIVITDDVPNDI
                     DFVGGLITETFQTPLAHVNILSQSRKTPNMALPKASQTPAIKQLIGTLVRLEVNDGGY
                     TLRPASLEEAEAFWDSRVAATPKLIPRLDSQFNSLLDLKNASFADLPRIGAKAAQMAE
                     LFKVDQRNSQCAEGAAFALPEGAFAVPMAFYLDHMRTSGAQTLLNQYLADRQFQTDLT
                     YRKAALAQVQEKILQSPVDPALLAQINSWAKARFGKKTLRFRSSSNTEDLAEFNGAGL
                     YESLSAKLDKPDESVEIALRTVWASLWNLRAYEERENSHVEQSQVAMAVLVHRSFPDE
                     RANGVAVGRNVLDVTRTDQYYFNIQAGEASVTNPAPGVVTEELIYQWPPRTPTLTYQS
                     YSSLLPNQRVITPTENRALACSLDAIQRHFRALLDPNGQDRWFTIETEFKFLGEERQL
                     LIKQARPYKLPPLDIPNDCREIE"
     gene            complement(4672..6075)
                     /gene="trkA"
                     /locus_tag="PKZ52_02020"
     CDS             complement(4672..6075)
                     /gene="trkA"
                     /locus_tag="PKZ52_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNG58571.1"
                     /translation="MKIIILGAGQVGGTLAANLASESNDITVVDRNETHLRELRDSLD
                     IGTVFGHASHPDVLLRAGIEDADMLIAVTSDDEINMVACQIAHNLFNTPTKIARVRSQ
                     EYVSYPGLFGKNAFPVDVIISPEQLVSDYIHRLVEQPGALQVLDFAQGLVQLVAVRAY
                     YGGPLVGQELRFIRKHMPNVDTRVAAVYRRNRPITPQGNTVIEADDEVFFIAAKEDIR
                     AVMSELRRLERPYKRIVIAGGGNIGMNLALKLETRYAVKVIEFNRTRATFLSEKLEHA
                     IVLNGSASDQNLLVEENIEDSDVFLAVTNSDEANIMASMLAKRLGARKVLTLISNPAY
                     VDLVQGGEIDIAISPQQATIGSLLTHVRRGDIVNVHSLRRGAAEAIEVIAHGDSKSSK
                     VVGKNIEDIDLPEGATIGAIVRKQKNAQGADIGHEVLIAHDRTRVESGDHVIVFLVDK
                     RYTRDIERLFQVGFSFF"
     gene            complement(6079..7410)
                     /gene="rsmB"
                     /locus_tag="PKZ52_02025"
     CDS             complement(6079..7410)
                     /gene="rsmB"
                     /locus_tag="PKZ52_02025"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNG58572.1"
                     /translation="MKRPALPQDVRALAALALHEVFDQGRSLSQVLPEYSQACPERDR
                     GLLKELCFGSLRYATTYLLVLQELIPTPLKRKDGDVQALLLMGLYQLEHTRIPDHAAL
                     SATVEASRLLHKDWAAGFINGVLRRYQRERELLAEKFAKNPNYRYAHPQWLLDRIRSA
                     WPDHWQEVLEANNQHPPMTLRVNSQKTDRATYLAKLAAAEIDASPCAYSPVGITLAEA
                     LPVEALPGFSEGEISVQDEAAQLAAYLVAPKAGERLLDACCAPGGKTCHLLEIAPAAD
                     LLAIDIDPTRLQRVTENLDRLGLEAQLKAWDLTDVNGWWDGREFDAILLDAACSATGV
                     VRRHPDIKHLRRASDLAPLAKLQQRLLKALWQTLKPGGRLVYATCSVLPDENSKQVQE
                     FLRLQPDAEHSELAVDWGIPQAFGRQLLPQAGGQDGFYYAVLHKAERSNGS"
     gene            complement(7407..8351)
                     /gene="fmt"
                     /locus_tag="PKZ52_02030"
     CDS             complement(7407..8351)
                     /gene="fmt"
                     /locus_tag="PKZ52_02030"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNG58573.1"
                     /translation="MPPAPLKIIFAGTPVFAALHLEALLGSCHEVVAVYTQPDRPAGR
                     GKQLQASPVKSLALQHQLPVCQVSSLKTPEAQAELAHWGADIMVVVAYGLLLPQAVLD
                     TPRLGCINVHASLLPRWRGAAPIQRAIEAGDASTGITIMQMDAGLDTGPMLAITPCPI
                     LPTDTATDLHDRLGSLGPAALLSALDDLACGRAAPQAQPSEGVTYAHKLTKEEAAIDW
                     TLDAQLIERRVRAFNPAPVAYFHLGQERVRVLGALLENGLSGVPGEIMRLDASGLYVA
                     CGSGGLRIETLQLPGKNPVAVKALINGSHPFALNQVLQ"
     gene            complement(8373..8876)
                     /gene="def"
                     /locus_tag="PKZ52_02035"
     CDS             complement(8373..8876)
                     /gene="def"
                     /locus_tag="PKZ52_02035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNG58574.1"
                     /translation="MAQLPILKFPDPRLRTKAKPVAQVDARVRRIIDDMLETMYAAPG
                     VGLAATQVNIHEQIIVIDVSENKDQPLVFINPRIETLDSALLGYEEGCLSVPGFYETV
                     ERPEHIKVHALDRDGKAFEMVPSGLLAVCIQHEMDHLSGKLFVDYLSSLKRNRIRAKL
                     EKQQKAP"
     gene            9031..10047
                     /locus_tag="PKZ52_02040"
     CDS             9031..10047
                     /locus_tag="PKZ52_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNG58575.1"
                     /translation="MKKRYLGLIAACLLSGHLWAESPKLRSDVPDTYTVVKGDTLWDI
                     SGRFLQNAWMWPEIWHVNPQISNPHLIYPGDVIKLVYIDGKPRLTLDRGIKKLSPQAH
                     VLDQNAAIATIPLDRIASFLSKARIVSPDELKKAPYVVSGESEHLLSGKGDKVYARGT
                     FDPDVSAYGIYREGQTFKDPKTRELLGVYAANIGGAKMRALDGDIATLLSTRSQEEIR
                     INDRLLVDDGQGIDSNFVPSAPASEIDGEIIFVEGGVTQVGKMNVVLLNRGVREGLAV
                     GNVLAVFKRGATVRDSQRGDRVTLPDERAGLVMVFKTFEKLSLGLILEAERPLSVSDK
                     VHNP"
     gene            10205..11389
                     /gene="dprA"
                     /locus_tag="PKZ52_02045"
     CDS             10205..11389
                     /gene="dprA"
                     /locus_tag="PKZ52_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNG58576.1"
                     /translation="MQMERALLMLQHLPNTTTNTYWLLLKAFPSLEYALEANPEKLHK
                     LLSEAAIAELLEHRRNPHGSRLTERVNRALDWCQREQVQLISINSDLYPAVLREIPKA
                     PALIYLKGRPQALALPQLAMVGSRSPSPSGVSIAREFAQHLAAGGLAITSGLALGIDQ
                     AAHEGALAAEGMTIAVMATGIDQVYPRRNQALAERIVAAGGALITEFPIGTAPQAAFF
                     PQRNRIISGLSAGVLVVEAAVKSGSLITARYALQHNREVFAIPGSIHNPLCRGCHALI
                     KEGAVLTESVDDIFAHLEGYLSLKWQELGLPSRPLSDAPSGLTLMERGELIENPLESV
                     VLAQLDFSPATLDELGERTGLPIGDLLTSLLTLELKGLASSFNGGYTRMAPTSLRPPL
                     LI"
     gene            11507..12070
                     /locus_tag="PKZ52_02050"
     CDS             11507..12070
                     /locus_tag="PKZ52_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNG58577.1"
                     /translation="MPASYHPRIQQAVRHLLVGGVLAYPTEAVWGLGCCLDDETAVAR
                     LLALKQRPVHKGLILVSGTLSHFDFLLERCSTEQRQTMQATWPGPNTWLVPHHNLVPA
                     WISGRHPSVAIRVSDHPIIGALSAALGGPLVSTSANPQGLTPARTALRSRVYFGSQVH
                     YCCGAVGRSNNPSQIRDLISGQIVRPG"
     gene            12147..13055
                     /gene="hemF"
                     /locus_tag="PKZ52_02055"
     CDS             12147..13055
                     /gene="hemF"
                     /locus_tag="PKZ52_02055"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNG58578.1"
                     /translation="MQAPDKHAVKNYLLGLQERICAQLAAADGAGSFVEDSWQRPEGG
                     GGRSRVLTDGTIIEKGGVNFSHVTGSQMPASATAHRPELAGRRFEAMGVSLVIHPNNP
                     YVPTSHANVRFFIAEKDGAEPVWWFGGGYDLTPYYGFDEDCQHWHQTARDACAPFGDS
                     VYADYKKWCDEYFFLKHRNEPRGVGGLFFDDLNAWGFERSFAFMQAVGDSYCQAYVPI
                     IERRKDLPFGEREKQFQLYRRGRYVEFNLVYDRGTLFGLQTGGRTESILMSLPPLVRW
                     EYNWTPAPGTAEAALYERYLMARDWV"
     gene            13058..13882
                     /gene="aroE"
                     /locus_tag="PKZ52_02060"
     CDS             13058..13882
                     /gene="aroE"
                     /locus_tag="PKZ52_02060"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNG58579.1"
                     /translation="MPTQRKPDRYAVVGNPIAHSKSPQIHSAFAAQTQQTMVYEKLLW
                     PLGGFAAEAQAFFSSGGAGLNVTVPFKEDAYAFAQQHTVRAQQAGAVNTLKRLENGQI
                     LGDNTDGAGLVADLKRLGWVLAHKKILLLGAGGAARGVIGPLLAENPVSITIANRTPS
                     KVAQLVTQFGNNPLMASSLEDLSGPFDIIINGISAGLHGEMPELPSRIWGPHSRAYDM
                     VYGPSRTPFLTLAAEQGAVALADGLGMLVGQAAEAFFLWRGLLPAVEPVLAMLRKV"
BASE COUNT         3013 a         3947 c         3933 g         3080 t
ORIGIN      
        1 gggcggcatc cttgccgcca ttattaaccc ggcactaaat gccgccgggt taatatttca
       61 acaagctgtt aagcgttaac ctgttgcttc ggcgtggtga gcgttaaggc tgcgccgagg
      121 aataaaaacc cggcgagcat aaataaaccg ctgtcggtgc tggcggttgc atcttttaac
      181 cagcccacta aataagggct taaaaagccc gctaaattcc ctaaggaatt aattaacgca
      241 ataccagctg ccgccgccac gccgcctaaa atagccgtgg gcaggctcca aaacagcggc
      301 aaaatactga ttatcccggc ggtggcaagg cttagcgccg ccatggccaa aagggtattg
      361 tgcgcccaca gtgcgcttaa tattaagcct gctccaccca gcagcgcagg cacggccaaa
      421 tgccagcgcc gctcccggtg tttgtcggcg ctgtggccta ataaaatcat tgtgatgctg
      481 gcaactgcat agggaattgc cgtcagccag cccacttctg ccgaattttc aacgcctaaa
      541 ttatgaatga gggtgggcag ccaaaaactc acgccgtaca gccccataac aaaacaaaaa
      601 taaataccgg ccatcagcca tactttgctg tcggctaaca tatggcgcgc cgaaagggta
      661 gatttgcttt gttcctcgcg gcggatattg ttatctaata aggttttttc tgcatcgttt
      721 agccaatggg cgctgtgaat gccatcggcc agaacaataa ttaccacaat gcccaataac
      781 agcgaaggta tggcctctaa aataaacatc cactgccaac cggccagccc gtaaacgccc
      841 ggcaggctcg cgccgatcca gccggacagc ggcccgccaa tgactccgga aataggcacg
      901 ccggtcataa aaagcgcggt aattttgccg cgccggtggg cgggaaacca ataggttaaa
      961 tacaaaataa caccggggaa aaacccagct tccgcagcgc ccaataaaaa acgcagaata
     1021 taaaaactta agggtgtggt cacccacacc atggcagcgg acactaacgc ccaggtaatc
     1081 ataatgcggg caatccacag gcgcgcaccc actttatgca gggcgatatt gctgggcact
     1141 tcaaaaataa aataaccgat gaaaaaaatc cccgccccca agccgtatac agtttcactg
     1201 aatttaagat cattgagcat ttgcaatttt gcaaaaccta cattgacgcg atctaaataa
     1261 gacgccacat aacagagaaa taaaatgggt aataagcgcc aggtgacacg ggcgtagagc
     1321 gctgtttcat cgatgggatt ggtgtgcatg ggggatgctc tggaagataa gacgttaaga
     1381 agataagacg ttaaatggcc cttggcctat caatataaag ggcctgggcc actgccggcg
     1441 cccagtaata aaaatgaatt tatcaggctt tgcgcacagt attacaaatt atcacagctt
     1501 cagtatgtcg ttttgatcaa ggttaaggta ggttccccca ttatttacca cttctggaga
     1561 aaccccatgg cgcgtctatt ttttatggct ctgtgctgtg ccttaagcgc ctgcgtggcg
     1621 ccggaaaagg acagcaatag tcagttgtgg attaacgagg tcgtcaccga caacgatggc
     1681 gtggccatag acgaaaacgg ccagaccagt gatttttcga gcttattaat actggcaccc
     1741 agagcctagc cttaagcgat tttgccattg ccgatgaggg cggcgtattc gccagcctac
     1801 ccgatgaact attagcgcct ggcggcatta tcaggctgtg ggcggatgat gatgcgaaac
     1861 tcggcagcta ccatttgccg tttaaattaa gcgcacaagg ggaaaccctg acgctgcgca
     1921 ataaactcgg tgagtcttat cagcaggtaa aaatcccggc actgggcatt aacgattcct
     1981 acagccgttt cccgtctggc aaaggggcgt ttgctatttg ccgctacgcc acacccaatc
     2041 gcagtaatgg caccgcctgc caatccgtca gccaccctgg gcttaccgag tcggtggtgt
     2101 ttaagccatt tgataccgcc cagtggccga gtcttacgcc gccgagtttg ggtattaatg
     2161 aattagcact attacccgct caatttattg aggtgaaaaa tttcagccaa cagttcgtgg
     2221 atttaactca atttaaatta attctcaccg cgtacccgcc gaatgcgccc ctgccgggtt
     2281 ttaatcacca ccacagcgtg cccctgcccg cccttagcct tgcccccggc gcaattgcca
     2341 ctattcctat agacggcgac caggtgcagg caattgctca gcaagccttt aatgaaggtg
     2401 ttgccgtgtt atacgaccgc aacacacaaa gccctgtgga ccaagtgccg tttatgcact
     2461 ggcccctcaa ccacgctttg gcgcgccagg agcattatcc ttaccgcctg cgtttttgtg
     2521 ataacccaac ccccaaccaa gatggcgcct gcgccgaaac ccagcagcgc cccatcggta
     2581 atcgcagccg cgggctttac acccccggtg attttgccgc gctggcggca ggcagtgggt
     2641 taaatcaaat tcagtcagtt aaatttgtgt tggatttaca gcggcaaaac gccgtgcatt
     2701 ttttaggttc ccgggagtgg cccctgcact atacgtttgt gcgggaaatt attgacggcg
     2761 atacccgcct taaccgctgt gagaatgaag actaccgctt atttatgaac ggctgggcgc
     2821 gcttttcagt ggagaattat tttaataaca ctacgcgccg ctaccattta ggcaccttaa
     2881 gtttttaccc caacgcccaa ttagccaatg tggaatttac ctttggcgat gaaattaccg
     2941 ccgcgcaaat gcgcgctgcg ttctacgccg tcaccgccgc cactgccaat ccatttaatt
     3001 ggtccctaag gccccaggat aacccacagg ttgagcgcgc ccgcgctatt gaaggcacct
     3061 taccgatagt tggcccccaa gcgccgtttg ccaatttagt tttccagggc cttgcgccgg
     3121 gtgtggccta tggcacccta aaaattattc ccaccaccga attaacctcg gcaagccttg
     3181 ggctgcgcac cattgtgatt accgatgatg tgcccaatga catagatttt gtcggcggct
     3241 taattaccga aacttttcaa acaccgcttg cccacgtaaa tattttaagc cagagccgta
     3301 aaacaccgaa tatggcccta cccaaggcga gtcaaacgcc ggctattaaa caattaattg
     3361 gcacgctggt gcgcctggag gtaaacgacg gcggatacac cctgcgcccg gcgagccttg
     3421 aagaagccga agccttttgg gatagccgcg ttgccgcaac gcctaaatta attccccgtt
     3481 tggatagcca atttaattcg ttgctggatt taaaaaatgc cagtttcgcg gatttaccgc
     3541 gcataggtgc caaggctgcg caaatggcgg aattatttaa agtcgatcag cgcaatagtc
     3601 agtgtgcaga aggtgccgca tttgccctgc ctgaaggtgc ctttgccgtg cccatggcgt
     3661 tttatttgga tcatatgcgc accagcggcg cgcagacgct gctcaaccaa tatttagcag
     3721 accggcaatt tcaaaccgat ttaacctatc gcaaagcggc ccttgcccag gtgcaagaaa
     3781 aaattctgca atcaccggtt gatcctgcac tgctggcgca aattaatagc tgggccaaag
     3841 cgcgttttgg caaaaagacc ctgcgttttc gcagcagtag taacacggaa gatttagcgg
     3901 aatttaatgg cgcggggtta tacgaatccc tcagcgccaa attagacaaa ccggatgaat
     3961 cggtggaaat tgccctgcgc actgtctggg cgagcctgtg gaatttacgc gcgtatgaag
     4021 agcgggaaaa ttcccatgtg gaacagagcc aagtggccat ggcggtgctg gtgcaccgct
     4081 cctttcccga tgagcgcgcc aatggtgtgg cggtggggcg caatgtgttg gatgtgaccc
     4141 gcacggatca atattatttt aatatccagg ccggtgaagc gagcgtgact aatcctgccc
     4201 ccggtgtggt aacagaagaa ttaatttacc aatggccgcc gcgcacccct acattaacct
     4261 accaaagtta cagcagtttg ctgcctaatc agcgggtaat taccccaacg gaaaatcgcg
     4321 ctttagcctg tagcctggat gcaatccagc gccactttcg cgcgctgctc gatcccaacg
     4381 ggcaggatcg ctggtttact atagaaacgg aatttaaatt cttaggtgaa gagcggcaat
     4441 tgctgatcaa acaagcgcgg ccctacaaac tgccgccgct ggatattcct aatgattgcc
     4501 gggagattga gtaaaggcca ttaaagcagc ggctagagcg tttttcggat aattaattcg
     4561 cggtgtggca gtgatgatac ggattatttc aaaatgtcaa aaggaccttg ttgacgagct
     4621 agggcgcgca aaagccttgc gcacgggtta ttggcctgca attgcttggg cttagaaaaa
     4681 cgaaaacccc acctggaata agcgctcaat atcccgcgtg tagcgcttat ccactaaaaa
     4741 tactatgaca tggtcgccgg actccacgcg ggttctatcg tgggcgatta atacttcgtg
     4801 gccgatgtcc gcgccctggg catttttttg tttgcgcact atggcgccta tggtggcgcc
     4861 ttcgggcagg tctatatcct cgatattttt gcctaccacc ttggaggatt tgctgtcgcc
     4921 gtgggcgatg acctcaatgg cctcggcggc gccccggcgc agggagtgta cgttgactat
     4981 gtcgccgcgc cgcacgtggg tgagcaggct gccgatggtg gcctgttggg gggagatggc
     5041 gatgtcgatc tcgccgccct gcactaaatc cacataagcg gggttggaaa tcagcgtaag
     5101 cactttgcgg gcgcctaggc gtttggccag catggaggcc atgatattgg cttcatcgga
     5161 gttggtgacg gccaaaaaca cgtccgagtc ttcgatgttt tcctctacca acagattttg
     5221 gtccgaggcg ctgccgttga gcactatggc gtgttcgagt ttttccgata aaaaggtggc
     5281 gcgggtgcgg ttaaattcaa tcaccttcac cgcgtagcgg gtttccagtt tcagcgctag
     5341 gttcatgcct atattgccgc cgcctgcgat cactatgcgt ttgtaggggc gctcaagcct
     5401 gcgcagctca ctcatcaccg cgcgaatatc ttctttagcg gcgataaaaa acacttcatc
     5461 gtcagcttca atcaccgtgt tgccctgggg agtgatgggc cggttgcggc ggtacacagc
     5521 ggcgacccgg gtgtccacat tgggcatgtg tttgcggata aacctcagct cctgccccac
     5581 cagcggcccg ccgtagtagg cccgcacggc taccagctgc actaaccctt gggcaaaatc
     5641 cagcacctgc agggcgccgg gttgctccac taagcggtga atataatcgc tcaccagttg
     5701 ttctgggctg atgattacat ccaccgggaa ggcatttttg ccgaacaggc cggggtagct
     5761 gacatactcc tgggagcgca cccgggcgat tttggtgggg gtgttaaaca ggttatgggc
     5821 gatctggcag gccaccatat tgatctcatc gtcgctggtg acggcgatca gcatgtcggc
     5881 atcctcaatg cccgcccgca gcaacacgtc cgggtgggag gcgtggccga acaccgtgcc
     5941 tatatccagg ctgtcgcgca gctcccgcag gtgggtttca ttgcgatcca ccacggtaat
     6001 gtcgttggat tcactggcca ggttggccgc aagggtgccg cccacttggc ctgcgccgag
     6061 gatgataatt ttcatagctt aactgccatt gctgcgctct gccttgtgca gaaccgcgta
     6121 ataaaaacca tcctggccgc ccgcttgggg cagcagttgc cgcccaaacg cttggggtat
     6181 gccccagtca accgccaact cactatgctc ggcgtccggt tgcaggcgca aaaattcctg
     6241 cacctgtttg ctgttttcat cgggcagcac cgagcaggtg gcgtacacca aacggccgcc
     6301 gggcttgagg gtttgccaga gggctttcaa caggcgctgt tgcagtttgg ccaggggcgc
     6361 aaggtcgctg gcgcggcgca agtgtttgat atccgggtgg cggcgcacca cgccggtggc
     6421 ggagcaggcg gcatccagca aaatggcgtc aaactcacgg ccatcccacc agccgtttac
     6481 gtccgttaag tcccaggcct taagctgcgc ttctagccct aagcgatcga gattttctgt
     6541 cacccgctgc aggcgtgtgg ggtctatatc tatagctaaa agatccgctg cgggggcaat
     6601 ttccagcaaa tgacaggttt tgccgccggg ggcgcagcag gcatccaaca ggcgctcacc
     6661 ggccttgggc gcaactaagt aggccgccaa ttgggccgcc tcgtcctgca cgctaatttc
     6721 accctcgcta aaacccggta gggcttccac gggcaatgcc tctgctaggg taatgcccac
     6781 agggctataa gcgcaggggc tggcgtcgat ctctgcggcg gcaagttttg ccaggtaggt
     6841 ggcgcggtcg gttttttggc tgtttacccg cagggtcatg ggcgggtgtt ggttgttggc
     6901 ctcaagcacc tcctgccaat ggtcgggcca ggccgagcgg atgcgatcga gcagccattg
     6961 ggggtgggcg tagcggtagt tggggttctt ggcgaatttt tctgccagta gctcgcgctc
     7021 gcgctgatag cgccgcaaca cgccgttgat gaagcctgcg gcccagtctt tatgcagcag
     7081 gcggctggct tccacagtgg cggaaagggc ggcatggtcg gggatgcggg tgtgttcaag
     7141 ctggtacagg cccatcaata acagggcttg cacatcgccg tctttgcgtt ttaagggtgt
     7201 ggggatcagc tcctgcagta ccagtaaata ggtggtggcg tagcgcaggc tgccaaagca
     7261 gagttctttt aacagccccc ggtcccgctc cgggcaggct tggctgtatt caggcaacac
     7321 ctggcttaag gagcggcctt ggtcgaagac ctcgtgcagg gctagagccg ccagggcgcg
     7381 cacatcctgg ggcagggcag ggcgcttcat tgcagtacct gattgagggc gaatggatgg
     7441 ctgccgttga tcagtgcctt cactgctacg gggtttttgc cgggcaactg caatgtctcg
     7501 atgcgcaggc cgccactgcc gcaggctaca tacaagccgc tggcgtccag gcgcatgatt
     7561 tcaccgggta cgccgctcag gccgttttcc aatagggcgc ctaacacccg cacccgctcc
     7621 tggcctaggt gaaaataagc cactggggcg gggttaaagg cccgcacccg gcgctcaata
     7681 agctgcgcat ctaaagtcca atcgatggcg gcttcttctt tggtgagttt gtgggcgtag
     7741 gtaacgcctt cgcttggctg cgcttggggg gcggctctgc cacaggccaa atcatccagg
     7801 gcgctaagca gggccgcagg ccctaggctt cccaagcgat cgtgcagatc agttgcggtg
     7861 tcagtgggca gaattgggca gggggtgatg gcgagcatgg ggccggtatc aagccctgca
     7921 tccatttgca taatggtgat gccagtgctg gcatcgcctg cttcgatagc tcgctggatg
     7981 ggcgccgccc cccgccagcg gggtaaaagg ctggcgtgta cattgatgca gccgagcctt
     8041 ggggtatcca gcaccgcttg gggcagcagc agcccatagg ccaccaccac cataatatcc
     8101 gctccccagt gggcaagttc cgcttgagcc tcgggggttt taaggctgct cacctggcaa
     8161 accggcagct ggtgttgcag ggcgaggctc ttcaccgggc tagcctgcag ctgtttgccg
     8221 cgcccggccg ggcgatccgg ctgggtgtag accgccacca cctcgtggca actgcccagt
     8281 agcgcctcca ggtggagagc ggcgaataca ggggtgccgg caaaaatgat cttaaggggg
     8341 gcagggggca tggctgatcc tgaaggggcg aattaagggg ctttttgttg tttttctaac
     8401 ttcgcccgga tgcgattgcg cttaagggaa gagaggtaat ccacaaataa cttgccgctt
     8461 aaatgatcca tttcgtgttg gatgcacacg gctaacaagc cgctgggcac catctcaaat
     8521 gccttgccgt cgcggtcgag ggcgtgcact ttgatgtgtt ctggccgctc aaccgtctcg
     8581 taaaagcccg gcacactcaa gcagccctct tcatagccca gcagggctga gtctagggtt
     8641 tcgatgcgcg ggttgataaa caccaggggt tggtctttgt tttccgatac atcaatcaca
     8701 atgatctgct cgtggatatt cacctgggtg gcggctaagc ccacaccagg ggcggcgtac
     8761 atggtttcaa gcatgtcgtc gatgatcctg cgcacgcggg cgtccacttg ggcaacaggc
     8821 ttagctttag tgcgcagcct ggggtcggga aactttagta tgggtaattg tgccattggc
     8881 ctttgtgaca aacgtctggt aaaaaggtga gttgcgctgc taccattgaa tgaatatcgc
     8941 gggtttgcag ggttctgtcc acgcgcctgg cggtggttgg gctattataa cgaacaacat
     9001 ccattggcct actggtacag gaccattcct atgaaaaaac gctacttggg cttaattgct
     9061 gcctgcctct tatcgggcca cctgtgggcc gaatcgccca agctgcgcag cgatgtgccc
     9121 gacacctaca cggtcgtcaa gggcgatacc ctgtgggata tctctggccg ctttttacaa
     9181 aacgcctgga tgtggcccga aatctggcat gtgaacccgc aaatctccaa cccccactta
     9241 atttaccccg gcgatgtcat caagctggtg tacatcgacg gcaagccgcg tttaaccctg
     9301 gatcgcggca tcaaaaagct ctctccacaa gcccatgtgt tggatcaaaa cgctgccatc
     9361 gccactattc ccctggatcg tatcgccagc ttcttatcta aagcccgcat agtctctccg
     9421 gatgagctga aaaaagcccc ctatgtggta tccggtgagt ctgagcatct gttgtccggt
     9481 aagggcgaca aggtttacgc tcgcggcacc tttgacccag atgtcagcgc ctatggtatt
     9541 taccgggagg ggcaaacctt taaagacccc aaaacccgcg agctgttggg tgtctatgcg
     9601 gccaatatcg gcggcgccaa aatgcgcgct ctggatggcg atatcgccac cctgctgtcc
     9661 acccgcagcc aggaggaaat ccgcatcaac gaccgcctgt tggtggatga cggccagggc
     9721 attgactcca acttcgtacc ctccgccccc gccagcgaga tcgacggcga aattattttt
     9781 gtagagggcg gagtaaccca agtgggtaaa atgaacgtgg tactgctcaa ccgcggtgta
     9841 cgtgaaggct tggcggtggg caatgtgcta gcggtgttta agcgcggcgc cacagtgcgc
     9901 gacagccagc gcggcgatag ggtcacccta ccggacgagc gcgcaggctt ggtgatggtg
     9961 ttcaaaacct ttgaaaagct gagccttggg ctcatcttgg aggccgagcg gcccctatcg
    10021 gtgtccgaca aggtgcacaa cccttaaata aaaaaccccg cttcggcggg gttttttatt
    10081 gccgaggtaa atttccggct caaaaatacc cgagcatcca tccgtcgccc gcgaggctgc
    10141 aagactttac ccgcgcgagt taacccgtta aggtcgcgtt tttgactgcc tgcgagggat
    10201 ttctatgcag atggagcgcg cgctgctgat gttgcagcac cttcctaata ccaccactaa
    10261 tacctattgg ctgctgctca aagcatttcc aagccttgag tatgccctgg aagccaaccc
    10321 ggaaaaactg cacaagctgt tgtctgaagc ggccattgct gagctcttgg agcaccgccg
    10381 caacccccac ggcagccgtt taaccgagcg ggtgaatagg gctttggatt ggtgccagcg
    10441 ggagcaggtg cagctcatct ccatcaacag cgacctgtac ccggctgtat tgcgggaaat
    10501 ccccaaggcc cccgcgctta tctatcttaa gggtcgcccc caagcgctcg cattgcccca
    10561 gctggccatg gtgggcagcc gcagtcccag ccccagtggc gtaagcatcg cccgcgagtt
    10621 tgcccagcat ctggccgctg gcggcctggc catcaccagc ggcctagccc tcggtataga
    10681 tcaagccgcc catgagggtg ccctggcggc cgagggcatg accatcgccg taatggccac
    10741 tggcatcgac caagtctacc cgcgccgcaa tcaggccctg gcggagcgca tagtggccgc
    10801 aggcggtgcc ctgattaccg aatttcccat cggcaccgcc cctcaggcgg cttttttccc
    10861 ccagcgcaac cgcattatca gcggtttaag tgccggtgta ttagtggtgg aggcggcggt
    10921 caaaagcggc tcattaatta ccgcccgtta cgccctgcaa cacaaccgtg aggtgttcgc
    10981 cattcccggc tccattcaca accccttatg ccggggctgc cacgccctca ttaaagaggg
    11041 cgccgtgtta accgagtcgg tggacgatat ttttgcccat ctggagggtt atttaagttt
    11101 gaaatggcag gagttgggct tgccctcgcg cccgctgtct gacgccccat cgggcctgac
    11161 cctgatggag cggggggagc tgatcgaaaa cccgttggag tcggtggtgt tggcccagct
    11221 ggactttagc ccggccaccc tagatgaact gggcgagcgt acggggctgc cgattgggga
    11281 tttgttaacc agcctgctca ccctggagct taaaggcctg gccagcagtt ttaacggcgg
    11341 ttacacccgc atggcgccca cctcattaag gccccccttg ctgatctaaa tcagctgcag
    11401 taagttggcg caccttcaac tcccagccct gtgttgccgc cctcaggcgc tactggcctg
    11461 ggcttgcgtc tgcccactgc gtattaaggt ctgctgcttg agcctattgc ccgcctctta
    11521 tcatccccgt attcaacagg ctgtgcgcca tctgctggtg ggcggtgtgt tggcctatcc
    11581 caccgaagcc gtgtggggct tgggttgttg tttagatgat gaaaccgccg ttgcccgcct
    11641 gctggccctc aaacagcgcc cggtgcacaa gggcttgatt ctggtgagcg gcaccctatc
    11701 ccattttgat tttttgcttg agcgctgtag cactgagcag cgccaaacca tgcaggccac
    11761 ctggccaggc cccaacacct ggttagtgcc tcaccacaat ttagtacccg cctggatcag
    11821 cggccgccac ccatcggtgg ccatccgggt gagcgaccac cccattatcg gcgctctgtc
    11881 agcggccctc ggcggcccct tagtatctac ctccgccaac ccacagggcc ttacccccgc
    11941 ccgcacggcc ctgcgcagcc gcgtgtattt tggcagtcag gtgcactact gttgcggggc
    12001 agtaggtaga tcaaacaacc ccagtcagat tcgtgatcta atcagcggcc aaattgttag
    12061 gccgggttaa gggccggaac ttatcgcggc cgcacaggcc actggcctca ttcgattatt
    12121 ttaaaaaggc gccctagggg acacgcatgc aagcaccgga taaacacgcg gtaaaaaact
    12181 atttgttagg gctgcaagag cgcatttgcg cgcagttggc cgccgccgat ggcgcaggca
    12241 gttttgtgga agacagctgg cagcggccgg agggtggcgg tggccgctcg cgggtgttaa
    12301 cggatggcac catcatcgaa aaaggcgggg tgaatttctc ccatgttacc ggcagccaaa
    12361 tgcccgcctc ggccaccgcc caccggccgg agttggcggg tcgtcgtttt gaagccatgg
    12421 gggtgtcttt ggtgattcac cccaataacc cttatgtgcc caccagccat gccaatgtgc
    12481 gtttttttat cgctgaaaag gacggcgccg agccggtgtg gtggtttggc ggcggttacg
    12541 acctcacccc ttattacggc tttgacgaag actgccaaca ctggcaccaa accgcccgcg
    12601 atgcctgcgc gccgtttggc gattcggtat atgccgatta caaaaaatgg tgcgatgaat
    12661 acttcttttt gaaacaccgc aacgagccgc gcggtgtagg cggtttgttc tttgatgatc
    12721 ttaacgcctg gggctttgag cgcagtttcg cctttatgca ggcggtggga gacagttatt
    12781 gccaggccta tgtgcccatc atcgagcgcc gcaaagacct gccctttggc gagcgggaga
    12841 aacaatttca actctaccgc cggggccgtt acgtggagtt taacctggtg tacgaccggg
    12901 gcactctatt cggcctgcaa accggcggcc gcaccgaatc catccttatg tccctgccgc
    12961 ctctggtgcg ttgggaatac aactggacgc ctgcgcccgg cacggcggaa gcggcgctct
    13021 atgagcgcta tttgatggcc cgcgactggg tgtaaccatg ccaacacaga gaaaacccga
    13081 tcgttacgcg gtggtgggca accccatcgc ccactccaaa tccccgcaga ttcacagcgc
    13141 atttgccgcg caaactcagc agacgatggt gtatgaaaaa ctgctctggc ctttgggtgg
    13201 ttttgctgca gaggcccagg cgttttttag tagtggcggt gcgggtttga atgtcaccgt
    13261 accctttaaa gaagacgcct atgcctttgc ccagcagcac acggtgcggg cccagcaggc
    13321 cggggcggtg aacacactca aacggctgga aaacgggcag attctcggcg ataacaccga
    13381 tggcgcaggc ctagtggccg atttaaaacg cttaggctgg gtcttagccc acaagaagat
    13441 tctcctcttg ggcgcaggcg gcgccgcgcg gggtgtgatt gggccgctgc tggcggagaa
    13501 cccggtgagc atcaccatcg ccaaccgcac cccctcaaag gtggcccaat tggtgaccca
    13561 gtttggcaat aacccgctta tggccagcag ccttgaagac ctgagcggcc ccttcgatat
    13621 catcatcaat ggcatatcgg caggcttgca tggcgaaatg cctgagcttc ccagccgcat
    13681 ctggggcccc cacagccgcg cctacgatat ggtctacggc cccagccgca cgccgttttt
    13741 aaccctcgca gcggagcagg gcgcggtggc tttagccgat ggcttgggta tgttggtggg
    13801 ccaggcggcg gaggcgtttt tcctctggcg ggggctattg cctgcggtgg agccggtatt
    13861 ggcaatgctg cgcaaggttt agggaacctc tgaaaaatta cctcactcgc cctagccacg
    13921 ttgaatttgg caaaaaaatg ctcatttact gcgtgtaaac tcggcaattg ctc
//
LOCUS       DAOQGS010000030        13918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2040_length_13918_cov_4.818943, whole genome shotgun sequence.
ACCESSION   DAOQGS010000030 DAOQGS010000000
VERSION     DAOQGS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_13918_cov_4.818943"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..922)
                     /locus_tag="PKZ52_02065"
     CDS             complement(95..922)
                     /locus_tag="PKZ52_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNG58580.1"
                     /translation="MKQITLLCALFCALALSACGKSGGSPSGSSSSSSSSSSSSSSGV
                     PYTYLGSVQKNLAITSAITGITYPYHVYLPYNYAISAKNYPIIYGTDAQWVFNHFSQT
                     LDARNKDVIFIGIEEGPLNSNRRFSDFLGPGVNDYMRFFKTEFAPLMESTYRFNHERT
                     FIGTSLGGLLGSTLLTREPIEEPYFKNYMLFDGSFFAIDVAGINDELSRYNASKKMNI
                     TLILTSANPGNEESVNGLQERYTARAYEGLTIYRRSFAIPHNDVANPSFDDTIDLLY"
     gene            1395..4112
                     /locus_tag="PKZ52_02070"
     CDS             1395..4112
                     /locus_tag="PKZ52_02070"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNG58581.1"
                     /translation="MIIVLIGIIAWLFYLLSRLNSLDAQVKNLSQELLKLKHQLSPQA
                     KNPPQEVQETPPVVVAPKKPLAEVINESLGSAPPELRELPQRQPLQASAPPQIASQQT
                     QATPTKPRPPITASAPRTPNGIARSVASLASFFTQGNPIVRVGMVVLFLGVSFLVKYA
                     AGQGLLPIELRLTAVIVIAAALLILGWKTRARAGGYGLVLQGGGIAVIYLTLFAAAKL
                     YGLIPLAGAFGLLFAVVMLGVALALVQNAQVLAILATAGGFLAPILTSSGSGSHVGLF
                     SFYLILNLGILAIALYKTWRLLNWVGFMFTFVITSLWGVLKYSPEVYASTQPFLAAFF
                     VLYLTVAILFSLKQPANLKGLVDGSLVFGLPLIAFGLQTALLKHTQYGLALSALALAV
                     IYIALAYGLARQYKSSQRVLIEAFLALGIGFATLAIPLALDASWTGISWALEACGLIW
                     VGLRQERLRPRSAGYLLQAAAAFSLVAINGVHTGDVPIIRGDFLALVLLAVSSFTTAY
                     LLHIYRTRVKALELMVASLALMFGTLWWMVAGIIELRAHTPSAQHFAGIIIFAALSAQ
                     VMVQLGLKLTWPQLNRCVFALLPFLILFCVQQFIIDSSELHPAQGFNSLAIILALAVG
                     YRFLWLQQASDVAAQKQQNVGLTTWHVFTAWWLFGLIFWEIAWQQQHVHLHGTGAALI
                     WFAAISLPIVALLTAAKRVFWPFNAYEEAYKNWVPAPLFLLATLWFIAACHKTGAAAA
                     NYVPLLNPLDLAQCAVILVFAYAAKEEFLAALGFSARAVQAVFLGLLGFIWINVVVLR
                     AVGHYQQIPYELSALWQALPVQMALSILWTACALLVMNLSRRWQQRQLWILGAGLLAV
                     VVIKLAAKDLSGSGTLAGIISFMVVGALMLLIGYLSPIPAKRTQAPNHE"
     gene            4105..5544
                     /locus_tag="PKZ52_02075"
     CDS             4105..5544
                     /locus_tag="PKZ52_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HNG58582.1"
                     /translation="MNKITRPPGLCALFLLFSAITAQAGTTPLFQINAEPGTFIQVPL
                     THQVYQFNPPSDAAFVVLDGKQNSLPSIMSAPSPNNTEPSVQIRSQQLAFFAVPAETS
                     TQALRQVLSTQNQVLNYGSKQISQHIETQDTLSTNTPDFYLIDLRNLEQGINALIIDW
                     DNKTQNSFLQVQVDASNNLQDWQKLTQASLVQVQEQEQSLIHNRIPVSIAKQAFAYLR
                     LSLMRGAEQLHIQKISAEERLYSAPPQELPQEHWSLAGTLAKEQTSLYRPSSQGAAYP
                     VRAFEFSRDESTPAEFLALNLGMQTYADRLRVLSRTEARGQWELIYQGIWFNTQMGEE
                     WQTSAPIKLGGNRAQFWRLEFAAQQAIDAPELLFSWHPTQLQFIANNAPPFVLGLSDD
                     NGSVNRAQVFHELLAKHSQQGSKAQWISGQLSPKTRPQAQAAALHNSPNNPNWRQWLF
                     WLALTGTVALLVFFALRLIKQLNLPANEP"
     gene            complement(5694..6281)
                     /locus_tag="PKZ52_02080"
     CDS             complement(5694..6281)
                     /locus_tag="PKZ52_02080"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNG58583.1"
                     /translation="MSKRILVIQGHPDKNSARLGSALLNAYAEGAREAGHQVRQLIVA
                     ELDFPLLRTQADWETGPVPAGLIQAQDDIFWAQHIVLFFPLWLGDMPALLKAFLEQVA
                     RPGFAFTSEGDNPFAHKGLKGRSARVVVTMGMPALVYRWYFRAHSLNSLERNILGFVG
                     IGPIDETLIGMVGAMQPKDRQKWLRKMALLGRQGI"
     gene            6763..7899
                     /locus_tag="PKZ52_02085"
     CDS             6763..7899
                     /locus_tag="PKZ52_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG58584.1"
                     /translation="MTRTTSPLKSILIGTGAVLVILAIIAGIKGGQIGFMIKQGQSFT
                     QPPETITSATAATQAWPNIFKAIGTVEAAEGITISAEVAGKVKRIAFKSGEYVRAGEV
                     IIEQEMGNEEAQLAAFTARLKLAQANFERLSQLKQKNTVSQSELDLARQQKESAQGDV
                     DNLKTTLQKKILRAPFDGRLGIRQVDLGQDLQVGAPIVSLQASNRVRVNFPAPQAWIV
                     QLKKGMAVSLDVGKGPDHLIKTSISAIGAEINPVTRNATVQASLENTDNQLIPGMGVK
                     VEVSLSEPREVLAIPGTAIVYAPFGDTVYVVEAGEKPNTLKARQQFIRTGEQRGDFVE
                     VLDGIKAGQKVVAAGAFKLFNGQAVVESTQPTPEYQLNPKPADK"
     gene            7913..11005
                     /locus_tag="PKZ52_02090"
     CDS             7913..11005
                     /locus_tag="PKZ52_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG58585.1"
                     /translation="MNFTDIFIRKPVLALVVSLLILLAGIQSWKDMTVRQYPRSDIAV
                     IKISTLYVGANAELVRGFVTTPIERAIASAGGIDYIASESAMSMSTVNVHLRLNYDPL
                     KAMTEISAKVNQVRGDLPPEAKIPTIVIEAADSQFASAYLSFSSTILDQNQITDYLTR
                     SVQPRLTAIAGVQKADVLGGRVFAMRIWLKSAQLAALNISPAEVRQALAANNVQAAIG
                     TTRGAYTQVNLSADTDLKNVEDFKKLVIKNTEEGVIRLEDVADVVLGAEDYSVEVGFS
                     GDTAVFMGVWVAPNANSLDVIAAVTKEMEAIKADLPTGLNGRVAYDGTKYINAAIHEV
                     FTTLTETLLIIIGVIFLFLGFSRSVIIPVLAIPLSLIGAIFLMKLFGFSLNLLTLLSI
                     VLSVGLVVDDAIVMVENIERHIQEGLKPFKAALVAARELGGPIIAMTVTLISVYVPIG
                     LQGGLTGTLFREFAITLAGAVTISAIVAITLSPMLGSRLLKPHKTIEAPLAKIFDRFQ
                     AYYSRKLEGVLHSRSGVYVFWVLISLTCIPMFLMSARELAPMEDQGVIFGIVDAPANS
                     TIDQSSFYAKQVNKTFMGVPETDFTFQITFPTGGFSGMVTKPWNERDRSVFEILPEVQ
                     QNLSQMAGVRILPITPPPLPGGGDFPVELVLSSTADIKEINDYALKLQQKMNASGQFA
                     FSLIDVKFDQPQYELAIDREKVADLGLNLQSVVNDLSTLLGGGYVNRFNMAGQSYKVI
                     PQVKRGERLTPDDLKNLYIHGPKGNLIRVDEIASIQHETVPRSINRMQQLNSVKISAV
                     PMGPIDGPLTWLENEARKILPKNYTIDHTGESRQLRREGNTFVPAFSFAMVMIFLVLA
                     AQYNSFRDPIVILAGSVPLALFGALIFTFLKIPAPMPYYTDAFTCTMNIYSQVGLVTL
                     VGLIARNGILVVEFANRLQEQGLAKIDAIRQAAVVRLRPVLMTSIATIAGHTPLIFAS
                     GAGAEARNSIGLVLVFGMTIGTIMTLFVLPAVYVLIAKDHAADKKHLQELEAEAEALA
                     "
     gene            complement(11179..11757)
                     /locus_tag="PKZ52_02095"
     CDS             complement(11179..11757)
                     /locus_tag="PKZ52_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58586.1"
                     /translation="MTFDHFAQWRQTLIEVRQSDHCSLELREFLAGKIHHLHRAIHLP
                     AEERVEALAIYVMHYVEQVPTQLKTLARALQQTEAMPNSEYFLQIACDFFTHRPELIA
                     QGGGWITLLAEAYLAHRVVEEIYDRASLQGYFPAITQATVQANIIVHGVLGEGFANQL
                     DLAVFYAVESLYPANRTPALSLVKNKASAPLH"
     gene            12051..13238
                     /locus_tag="PKZ52_02100"
     CDS             12051..13238
                     /locus_tag="PKZ52_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNG58587.1"
                     /translation="MSVSRLLGRLMNHLGACTLVLATLTGLTGCGDKVPVVKNDYAGN
                     SGAEYYDKMGCAGCHGRGPGEGSYPITEENAGAITRSYIAQQMPPVAEGEAPKCTGAC
                     AVETAAYMKAWFTTPSTSSSSSSSTGSTNSSSSSSSSSSSSSSTSSSSSSSSSSSSSS
                     SSGITSSSSTSTSNSTSSTSSTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGGDATIG
                     KSQFEGQCGSCHGNRGEGGTSPGIDPRLYTEADLTRIIPTVNGMEHSSCAAANGCAAN
                     TAAYLVKTVAPLLQSGENLYKTVSSGACFDCHGNKGTGGTNPKSGVICTYLQTDACFE
                     ADVAENTFDAIKADVDSMTAIGRSTSSTRPKLCTDTIPAGADCSQNVSLYLKSLGWKR
                     NRQ"
     gene            13351..>13918
                     /locus_tag="PKZ52_02105"
     CDS             13351..>13918
                     /locus_tag="PKZ52_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNG58588.1"
                     /translation="MRIFSSLAIYFIAALSITACVATSPSNPSTNSSSSSTSSSSTSS
                     SSTSSSSSSSSSTSSSSSSSSSSGGDALVGKNIFEASCASCHGDLGQGGTSTGLNPHL
                     YTQADLTRIIPSIDMNHSSCTLANNCAANTAAYLISLNEIINTGENLYKTLGNGACLN
                     CHGDKGLGGLDIGICSYAHGEDCPNEFVL"
BASE COUNT         3409 a         3683 c         3377 g         3449 t
ORIGIN      
        1 gccaaattcg cctagctagt gcttcgccgc ctacattttt cagaggttcc ttaagataat
       61 ttttgatttt tagtgctcaa tagttacaaa ttgcttaata gagtagatcg atcgtgtcat
      121 caaaagatgg attggccacg tcgttgtggg gaatggcaaa cgagcggcgg taaatagtca
      181 gaccttcata ggcgcgggcc gtatagcgct cttgcaagcc attgaccgac tcctcattac
      241 ccggattagc actggttaaa atcagggtaa tattcatttt tttgctggcg ttatagcggc
      301 tgagctcatc gttaatgccc gccacatcaa tggcaaaaaa tgaaccgtca aacaacatat
      361 aatttttaaa atagggctcc tcaatcggct cgcgggtcaa taaagtgcta cctaacagac
      421 cgcccaggga tgtgcctata aacgtgcgtt catgattaaa gcggtaggta ctctccatta
      481 aaggcgcgaa ttcagtttta aaaaagcgca tatagtcgtt cacacccggc cctaaaaaat
      541 cgctaaagcg ccggttggag ttgagcggcc cctcttcaat accgataaaa ataacgtctt
      601 tattgcgcgc atccagggtt tgcgaaaagt ggttgaatac ccactgcgca tcggtgccat
      661 aaataatcgg atagttttta gcggaaatag cgtagttata gggcaaatac acatggtacg
      721 gataggtaat gccggtaatc gctgaggtaa tggccagatt tttctgcaca ctgcctaaat
      781 aggtgtaagg caccccactg ctggatgaac tgctcgagga actactggat gaactactgc
      841 cgctggggct gccgccagac ttaccacagg cgctaagggc cagggcgcaa aacagggcac
      901 acaacagcgt tatttgtttc ataggcgttt taatccggtg acaaatatat taagcggcgc
      961 gcaggcaaaa taaaatcagt gtttacctgc atcactgcag cttatcacgc ttaattacac
     1021 ctgctgcccg atggcaaaac gccccaccat aagcaaaaac gccgacacac agcccaccta
     1081 acacctacca aaaaccgccg cacattcaac cgcccgccca tttatgcagg tttagaccct
     1141 cccctgcgcc ccctcctgat agcccgtcgt ttaaccgcgc ccatgagaca gccgcccctt
     1201 gcccgcaata attcccctaa tggtgagccg agggtaaagc ggcggtaagc tgcatttatt
     1261 agcccgacgg gctttttgtg gaggtttata acccggcggg gtggaatcca ttcctatagg
     1321 ccaaatcaga ttccactata atcaactccg cttagctccg gcgccgcgtt cactctccca
     1381 tcaggatctt cacaatgatt attgtgctca tcggtattat cgcttggctg ttttacctgc
     1441 tgagcaggct caacagttta gatgcgcagg ttaagaacct cagccaggaa ttattaaaac
     1501 ttaaacacca actatcaccc caggccaaaa acccgccgca agaagtacaa gaaacgccgc
     1561 ccgttgtagt tgcgcctaaa aaacctctgg ccgaggtcat caatgaaagc ctaggctcag
     1621 cgccaccgga attacgagaa ttaccgcaaa ggcagcccct gcaggcgagc gcgccgcctc
     1681 agatcgcctc gcaacaaact caggctaccc ccaccaaacc ccgcccccca atcactgctt
     1741 ctgcgccgcg cacacctaat ggtattgcgc gatcagtggc tagcctggcg agttttttta
     1801 cccagggcaa tcctattgtg cgggtgggca tggtggtgtt gtttttgggt gtgagttttt
     1861 tagttaaata tgctgccggg cagggtttat tgccgattga attgcgctta accgctgtaa
     1921 ttgttattgc ggcggcctta ttaattctgg gctggaaaac ccgcgcccgc gccggcggtt
     1981 atggtttggt attacaaggc ggcggtattg cggttattta tttaacgctg tttgccgccg
     2041 ccaaactcta cggcctaata cctttagctg gagcctttgg tttattgttt gctgtggtga
     2101 tgttaggggt tgccctggcg ctagtacaaa atgcccaagt actggccatt ttagccaccg
     2161 caggcggatt tctcgccccg attctcacct ccagcggcag tggcagccat gtaggcttat
     2221 ttagttttta tttaatttta aacctgggca ttttagccat tgcgctgtat aaaacctggc
     2281 gcctactcaa ttgggtgggt tttatgttta cctttgtgat taccagcctg tggggcgttt
     2341 taaaatattc accggaagtg tacgccagca cccagccctt tttagcggcg ttttttgtgt
     2401 tgtatttaac ggtggcgatt ttattttccc ttaaacaacc ggccaacctt aaaggcctgg
     2461 tcgatggctc cttggtattt ggcctgccgc tgattgcctt tggcttacaa acggccctgt
     2521 taaaacacac ccaatacggc ctcgccttaa gtgcattggc cctcgccgtt atttatatcg
     2581 ccctcgccta cggccttgcc cgccagtata aaagcagcca acgggtatta attgaagcct
     2641 ttttagccct cggcattggc tttgccaccc tcgccattcc ccttgcctta gacgccagtt
     2701 ggacgggcat tagctgggcg ctggaggcct gcggattaat ttgggtggga ttgcggcagg
     2761 agcgcctgcg gccgcgcagt gcaggttatt tattacaggc tgcggcggca ttttccctcg
     2821 tggcaattaa cggtgtacac acaggggatg tgcctattat ccgcggcgat tttctcgcgc
     2881 tggtgttatt agcggtcagc agttttacga ctgcttattt actgcatatt tatcgcacgc
     2941 gcgtgaaggc gttggaatta atggtcgcca gccttgcgtt aatgttcggc actctgtggt
     3001 ggatggtggc gggcattatt gaactgcgcg cccacacccc tagcgcgcag cattttgcag
     3061 ggattattat ttttgcggcg ttaagtgccc aggtgatggt acaactggga ttaaaactca
     3121 cctggccaca attaaaccgg tgtgtatttg ccctgctgcc gtttttaatt ctgttctgcg
     3181 tacaacaatt tattatcgac agctccgaac tgcatccagc ccagggtttt aatagtctgg
     3241 cgattattct tgccctcgcc gttggctacc ggtttttatg gctgcagcag gcatctgatg
     3301 tggccgcaca aaaacagcaa aatgttggct taaccacctg gcatgtattc accgcctggt
     3361 ggttatttgg gctaattttt tgggaaatcg cctggcaaca acagcatgta cacttacatg
     3421 gcacaggcgc ggcgcttatt tggttcgcag ccataagcct gcccatagtg gccctgctga
     3481 cagcagccaa gcgagtattc tggccattta atgcctatga agaggcctat aaaaattggg
     3541 taccggcgcc cttattctta ctggccacgc tgtggtttat agccgcctgc cacaaaaccg
     3601 gtgcggccgc cgccaattat gtgccactgt taaacccgct tgatttagcc cagtgcgcgg
     3661 tgattttagt atttgcctac gcagctaaag aggaattttt agccgcccta ggctttagcg
     3721 cccgcgctgt gcaagcggtt tttttaggcc tattagggtt tatttggatt aatgttgtgg
     3781 tgctgcgggc tgtgggccat taccaacaaa ttccctatga attaagcgcc ctgtggcagg
     3841 ccctgccggt gcaaatggcc ctttccattt tatggacggc ctgcgcttta ctggtaatga
     3901 acctgtcccg ccgctggcaa cagcggcaac tgtggatact cggggccggc ctattagcgg
     3961 tggtagtgat taaattagcc gccaaagatt taagcggttc tggcactctg gcggggatta
     4021 tttcgtttat ggtggtgggc gctttaatgc tattgattgg ctatttatcg ccgattccgg
     4081 ccaaacgcac acaggcgccc aaccatgaat aaaatcaccc ggccccctgg tttgtgtgca
     4141 ctttttttat tgtttagcgc aatcacagcg caggcgggca caacgcccct atttcagatt
     4201 aacgccgagc caggcacttt tattcaagtg ccgctgacac atcaggtgta tcaatttaat
     4261 ccgcctagcg atgcagcgtt tgttgtgctc gatggcaagc aaaactcact gcccagtatt
     4321 atgagtgcgc caagccctaa caatacagag ccgagcgtgc agattcgcag ccagcaactg
     4381 gcattttttg cggtacctgc agaaacatcc acccaggccc tgcggcaggt gttgagcacc
     4441 cagaatcaag tgcttaacta tggctccaag cagatttctc aacacataga aacacaggac
     4501 accctcagca ctaatacacc cgatttttat ttaatcgacc tgcgcaactt agagcagggc
     4561 atcaatgcat taattattga ttgggacaat aagacacaaa atagttttct gcaggtgcag
     4621 gttgatgcca gcaataattt gcaggattgg caaaaactca cccaggctag tttagtgcaa
     4681 gtacaggagc aagagcagag ccttattcac aaccgtattc cagtatccat tgccaaacag
     4741 gcctttgcct atttgcgctt aagccttatg cgcggcgctg agcaattgca tattcaaaag
     4801 attagtgccg aagagaggct ctacagtgcg cccccgcagg aattgccgca ggagcactgg
     4861 agtctggcgg gcacactcgc aaaagaacaa accagcctgt atcgcccctc ctcccagggc
     4921 gctgcttacc cggtgcgggc atttgaattt agccgcgatg aatccacccc ggcggagttt
     4981 ttagccctta atttaggcat gcaaacctac gccgatcgcc tgcgggtttt atcccgcaca
     5041 gaagcccgcg gccaatggga attaatttac caaggtattt ggtttaacac ccaaatgggc
     5101 gaggaatggc aaaccagcgc gccgattaaa ctcggcggca accgggcaca gttctggcgt
     5161 ttggaatttg ccgcgcagca agccatcgat gcgccggaat tactattcag ttggcaccca
     5221 acgcaactgc aatttatcgc caataacgca ccgccatttg tactggggct cagtgatgac
     5281 aatggcagtg ttaatcgggc gcaagtattt cacgagctgc tggccaaaca cagccaacaa
     5341 gggagcaagg cgcagtggat aagcggccaa ttaagcccta aaacccgccc ccaagcccag
     5401 gcggccgccc ttcacaatag ccccaataac cccaattggc ggcagtggtt attttggctc
     5461 gccctcacag gtacagtggc gctgttggta ttttttgcgc tgcggttaat aaaacaatta
     5521 aacctgccag ccaatgaacc ctaaaatcga cgcaattatt caacgtaact attcaacaca
     5581 ttttactcac ctctcctgcg cgggcgggaa attcaggagg atttagccat ccggcagaat
     5641 cgatttgcgg tgcaacagcg gtgacattaa gggctcgcat agttatttaa aaactaaata
     5701 ccttgccgcc ccagcagcgc cattttgcgc agccattttt gcctgtcttt aggctgcatt
     5761 gcgcccacca tgccaatcag ggtttcatca atgggcccga ttcccacaaa gcctaaaata
     5821 ttgcgctcaa gggaattaag gctatgggcg cgaaaatacc agcgatacac cagggcgggc
     5881 atccccatag taaccaccac ccgcgccgag cggcctttaa ggcctttgtg cgcaaaggga
     5941 ttatcgccct cactggtaaa cgcaaacccc ggccgcgcca cctgctctaa aaacgctttt
     6001 aataaggcgg gcatatcgcc aagccagagc ggaaaaaaca acacaatatg ctgcgcccaa
     6061 aaaatatcat cttgcgcctg tataagccct gctggcaccg gccctgtttc ccaatccgcc
     6121 tgggtgcgca agagcggaaa atccaactcg gccactatta actgccgcac ctgatgcccc
     6181 gcctcgcggg cgccttcggc ataggcgtta agcagcgcac tgcctaagcg ggcagagttt
     6241 ttatccggat gcccttgaat gacgagaatg cgtttactca taaaaactcc attgtagtta
     6301 tgcacattgg gcctgggcat atgggttgga ggccagcgga caggcgacgt gccgccgcag
     6361 gtatggctat aagtgcccac ttgatgggta ccataatcgc ccctccagag ggccgcttga
     6421 tgtagcgcaa tagcctgtac gccttttaca ctcgcctttg ccgccaaaga tcagaaaaat
     6481 aaaccgcctg cccccaagcc gaatagaggc agcgagccta ggagccaggc tgatgttatc
     6541 ggcacaattt tttacgttat aaagcgcaac aaacctttcg gtatggtgta ctgctgtgca
     6601 acaatcgccg gggcgttgcc tccctaaatc tcaacctaac ctctcgtgtc ttcccctccc
     6661 ctaaacagcc tgctttttta gcgcttgttt gcgggtaccg ctcggctttt aggttgggtt
     6721 ttctttttat acgttccggt ttggcccggt ttggacagct ttatgacaag aaccacatcc
     6781 ccgcttaaat ccatcttgat tggcacaggt gctgtgttgg tgatcctggc catcattgca
     6841 ggcataaaag gtggccaaat cggctttatg attaaacagg ggcaaagttt cacccagccc
     6901 cctgaaacca tcaccagcgc caccgccgct acacaggcct ggccgaatat ttttaaagcc
     6961 ataggtacgg ttgaggccgc cgaaggcatc accatatccg ctgaggtagc cggtaaagtc
     7021 aaacgcattg cgtttaaatc cggcgaatat gtgcgcgccg gtgaagtgat tatcgagcag
     7081 gaaatgggca acgaagaggc ccaattggcc gccttcaccg cccgcttaaa actggcacag
     7141 gccaacttcg agcgcttaag ccagttaaaa caaaaaaata ccgtctccca aagcgagttg
     7201 gacctagccc gccaacaaaa agaatccgcc cagggtgatg tggacaactt aaaaaccacc
     7261 ctacagaaaa aaatcctccg tgcgcccttt gatggccgcc tgggtattcg ccaggtggac
     7321 ctagggcaag acttacaagt gggcgccccc atcgtgagtt tgcaggccag caaccgcgtg
     7381 cgggtgaact tccccgcccc tcaagcctgg atcgtgcaat tgaaaaaagg catggcagtg
     7441 agtttggatg tgggcaaggg gccagaccac ctgattaaaa ccagcatcag cgccataggc
     7501 gccgaaatta atcccgtcac ccgcaacgcc accgtacagg cgagccttga aaataccgac
     7561 aatcaactga tccccggcat gggagtaaaa gttgaagttt ccttaagcga accccgtgaa
     7621 gtcttggcaa tacccggcac cgccattgtg tatgccccct ttggcgatac ggtttatgtg
     7681 gtagaagccg gtgaaaagcc caacacccta aaagcccgcc aacaatttat ccgcaccggc
     7741 gaacagcgcg gggattttgt ggaagtgtta gatggtatta aagccggaca aaaagtagtg
     7801 gcggcaggtg cgtttaaatt atttaacggc caggcggtgg tagagagcac ccagccaacg
     7861 ccggaatacc aattaaatcc caagcctgct gataaataac tgaggctgcg ccatgaattt
     7921 caccgatatt tttattcgta aaccggtgct ggccttggtg gtcagcctgc taattttatt
     7981 ggccgggatc caatcctgga aggatatgac cgtgcggcaa tacccgcgct cggatatcgc
     8041 cgttattaaa atttccactt tatatgtagg tgcaaacgcc gagctggtgc gcggctttgt
     8101 caccacgccg attgagcggg ccatagcttc agctggcggc attgactata ttgcctctga
     8161 aagcgccatg agcatgtcca ctgtaaatgt gcatttgcgc ttaaattacg acccgctcaa
     8221 agccatgacg gaaatttccg ccaaggtaaa tcaggtgcgc ggcgatttac cgccggaagc
     8281 aaaaatcccc accattgtga ttgaagcggc ggattcacaa tttgcctccg cttatttaag
     8341 tttcagctca accattctcg atcaaaacca aattaccgat tacctcaccc gctcggtgca
     8401 accgcgttta acggcaattg ccggtgtgca aaaagcggat gtattaggtg ggcgcgtgtt
     8461 tgccatgcgt atttggctta aaagcgccca actggcagcc ttaaatattt ctcctgccga
     8521 agtacgccaa gccctggccg ccaataacgt gcaagcggcc ataggcacca cccgcggcgc
     8581 ttacacccag gtgaatttaa gtgctgatac ggatttaaaa aacgtcgagg attttaaaaa
     8641 gctcgttatt aaaaataccg aagaaggcgt gatccgctta gaggatgtgg ccgatgtggt
     8701 attaggcgcg gaggattaca gtgttgaggt gggtttctcc ggcgacaccg ccgtatttat
     8761 gggtgtgtgg gtggccccta atgccaactc cctggatgtt attgccgccg tcaccaaaga
     8821 aatggaagcg attaaagcgg atctgcccac cggtttaaat ggccgggtgg cctatgacgg
     8881 cactaaatat attaatgccg ctattcacga agtttttacc accctcaccg aaactctgtt
     8941 gattattatc ggcgtaatat ttttattcct aggctttagc cgctcagtca ttattcccgt
     9001 cttggcgatt ccactttcgc tcatcggcgc catcttttta atgaagttat tcggcttctc
     9061 actcaattta ttaacccttt tatccattgt gttatccgta gggctggtag tggatgacgc
     9121 tattgtgatg gtggaaaata tcgagcggca tattcaagaa ggtttaaaac cctttaaagc
     9181 cgctttggtt gccgcgcggg agttgggcgg gccgattatc gccatgaccg ttacgctcat
     9241 ttccgtatac gtacccatcg gtttacaagg cggtttaacc ggcaccctgt tccgggaatt
     9301 tgccattacc ctcgctggcg cagtgaccat ttccgccatt gtggccatta ccctttcacc
     9361 tatgctgggc tcgcggctgt taaaacccca taaaaccatt gaagcgccgc tagccaaaat
     9421 attcgaccgc tttcaagcct attacagccg caaactggaa ggcgtgttgc actcgcgctc
     9481 aggggtgtat gtgttttggg tgttaatctc cttaacctgc attcctatgt ttttaatgtc
     9541 cgcccgcgag cttgccccta tggaagatca gggagtaatt tttggcattg tggatgcgcc
     9601 agccaactct acgattgatc aatccagctt ctacgccaag caggtgaata aaacctttat
     9661 gggcgtgcct gagacggact tcacctttca aatcaccttc cccaccggcg gtttctccgg
     9721 catggtgacc aaaccttgga atgagcggga tagatcggta tttgaaattc tgccggaggt
     9781 gcaacaaaac ctaagccaga tggcgggcgt gcgcattctg ccgattacgc cgccacccct
     9841 gcccggcggc ggtgatttcc cagtggaatt ggtattaagc tccacagcgg atattaaaga
     9901 aattaacgat tacgccctta aactacagca aaaaatgaat gccagcggac aatttgcgtt
     9961 cagtttaatc gatgtgaaat tcgaccaacc ccagtatgag ctggccatcg accgggaaaa
    10021 agtcgccgat ttaggcctta atttacaatc ggtggtgaac gacttaagca ctctattagg
    10081 cgggggttat gtcaaccgct ttaatatggc gggccaaagt tacaaagtca ttccccaagt
    10141 aaaacgcggt gagcgcctaa cgccggatga tttaaaaaac ctctatatcc acgggccaaa
    10201 aggcaattta attcgcgtgg atgaaattgc cagcatccaa cacgaaaccg tgccgcgctc
    10261 tattaaccgt atgcaacagt taaattcggt gaaaattagc gccgtaccca tgggccccat
    10321 cgacgggcca ttaacctggt tggaaaacga agcgcgcaaa attctgccga aaaactacac
    10381 catcgatcac actggcgaat ctcgccaatt gcgccgggag ggcaatacct ttgtgcccgc
    10441 ctttagcttt gccatggtga tgatcttttt ggtgttagcg gcccagtaca acagctttcg
    10501 agacccgatt gtgattctgg ccggttcagt gcctttggct ttattcggcg cgcttatttt
    10561 taccttctta aaaatccccg cccccatgcc ctattacacg gacgccttta cctgcaccat
    10621 gaatatttat tcccaggtgg gcctggtgac gttagtaggc ctaatcgccc gcaacggtat
    10681 tttggtggtg gaatttgcca accgcttaca ggagcagggc ctggcaaaaa tcgatgccat
    10741 tcgccaggcg gcagtggtgc gtttacggcc ggtattaatg accagcatcg ccaccatcgc
    10801 aggccacaca ccacttattt ttgccagcgg tgcaggggct gaagcgcgca actccatcgg
    10861 cctggtattg gtatttggta tgaccatcgg caccatcatg accctgtttg tgttgcctgc
    10921 ggtgtatgta ttaattgcga aggatcatgc cgctgataaa aaacatttgc aggaactcga
    10981 ggcggaagca gaggccctcg cttaaatcca gccaacaaaa aagccacctt cgggtggctt
    11041 ttttgttttt aaggccaata atatccacgg ctcgaaaaca gcgctattgc agaggaatta
    11101 tcagtaaccc aactaccgaa tggcgcttta acacttaatc ctaaaaacct tagttaaact
    11161 cgcagcgagg tttttaggtt aatgcaaagg tgcagaggct ttgtttttta ccaggctgag
    11221 cgcaggcgta cgatttgccg ggtataaact ctccaccgca taaaacaccg ctaaatccaa
    11281 ctgattggca aacccctcgc ccaacacccc gtgcacaata atattggcct gcacggtggc
    11341 ttgggtgatg gccgggaagt atccctgcag tgaggcgcga tcatatattt cttccaccac
    11401 ccggtgggcc aaataggctt cagccaagag cgtaatccaa ccgccgccct gggcaattaa
    11461 ttccggtcgg tgggtgaaaa aatcgcaggc aatttgcaga aaatattcgc tattgggcat
    11521 ggcttcggtt tgctgcagcg cgcgggcgag ggtttttaat tgggtgggca cctgctctac
    11581 ataatgcatc acatagatag ccaaggcctc cacccgctct tctgctggca ggtgaatggc
    11641 ccggtgcaaa tgatgaattt ttcccgccaa aaattcccgc aactcaaggc tgcagtgatc
    11701 ggattgacgt acctcaataa gcgtttggcg ccactgggca aagtgatcaa aagtcataac
    11761 aattcagggg tccagcgcca aaacgtctta tgggtgcaca aattctgtgc ggcttattga
    11821 aaaataaccc aatccttcgt gaattcaagg cgttaagcta agtatgcgac gaatggaaat
    11881 agtcctcctg gacgaaagcc aacccccgcg gcccggagac tgtgcgccaa cgcacacacc
    11941 accctattga ggtgcggggc gctcacgcta aaagcagtat aaaatgaggg gatcgttttt
    12001 tagttttgtg ctggccctgc agcacatatt tgtcagattt ggagttgagt atgtccgtgt
    12061 cccgcctgtt aggccgccta atgaatcacc tcggcgcctg cactcttgtg ctcgcaaccc
    12121 tcaccggcct taccggttgt ggtgataagg ttccggtggt taaaaatgat tatgccggta
    12181 attcgggggc tgagtattac gacaaaatgg gctgtgctgg ctgccacggg cgcgggccgg
    12241 gggaagggtc ttatccaatc actgaagaga atgccggggc aattacacgt agttacatcg
    12301 cgcaacagat gccgccagta gcagaaggcg aagcacctaa gtgcaccggt gcttgtgcag
    12361 tggaaactgc agcctatatg aaagcctggt ttaccacgcc cagcaccagc tctagttcca
    12421 gcagctcaac cggctcaact aactcgtcct cctcaagctc tagctcctca tccagctcca
    12481 gtagtacttc gtcgagttca agcagttctt catccagctc tagctcgtca tctagtggca
    12541 taactagctc atcctccact agtaccagca actccactag ctcgacatct agcactagtt
    12601 cgtcgtcaag ctcctcttct agttcttcca gctctagctc ttcctcaagt tcatcctcta
    12661 gttctagctc ttcatcgagc agcggtggag atgcaactat tgggaaaagc caatttgaag
    12721 ggcaatgcgg cagttgtcat ggcaatcgtg gtgagggtgg tacttccccg ggaattgatc
    12781 cccgtcttta caccgaagct gacctaacca gaattatccc cacagttaat ggtatggaac
    12841 atagcagctg cgcggctgcc aatggctgcg cggcgaatac agccgcttac ctggttaaaa
    12901 ccgtcgctcc gctattgcaa agcggtgaga acctatacaa aactgttagt agtggggcct
    12961 gtttcgattg ccatggcaat aaaggtactg ggggaaccaa ccctaaaagc ggcgtaatct
    13021 gtacctacct tcaaactgat gcgtgctttg aggcagacgt tgcagaaaat acctttgatg
    13081 ctatcaaagc agacgtagac tccatgactg caattggtcg ctctacctcg agcacgagac
    13141 ctaaactatg cacggatacc attcctgctg gagctgattg ttcgcaaaac gtatccttgt
    13201 atttaaaatc cctcggctgg aaacgtaacc gccaataatt ccaaagctaa aataatcagc
    13261 tgcccaaaca cccagacttt cattggtctg ggtgtttttt atttagatac taaatttttt
    13321 cattcaaaca tttacctttc agggatttaa atgcgtattt tttcttcgtt agcaatttat
    13381 tttattgccg cgctgtccat aaccgcgtgt gtggctacta gcccctccaa cccatcgacc
    13441 aactcaagct catcctccac ctcaagttct agcacatcct ccagttctac ctcgtcgagt
    13501 tctagctcgt cctccagcac cagctcttct agttccagct catcgtccag cggcggtgac
    13561 gcgctcgttg gtaaaaatat ttttgaagcg agctgtgcta gctgccatgg cgatcttggg
    13621 cagggcggca caagcactgg attaaaccca cacttgtata cgcaagccga tttaacacgc
    13681 attattcctt caattgatat gaaccacagc agttgcaccc tcgccaataa ctgcgcagcc
    13741 aatacagccg cctatttgat ctcgctgaac gaaataatta atacaggcga gaatctttat
    13801 aaaacccttg gcaatggcgc ctgcttaaat tgtcatggtg ataaagggtt gggcggattg
    13861 gatattggta tttgcagtta cgcccatggc gaggattgcc cgaatgagtt cgtattaa
//
LOCUS       DAOQGS010000031        13740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2069_length_13740_cov_5.198246, whole genome shotgun sequence.
ACCESSION   DAOQGS010000031 DAOQGS010000000
VERSION     DAOQGS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13740
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2069_length_13740_cov_5.198246"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKZ52_02110"
     CDS             complement(<1..721)
                     /locus_tag="PKZ52_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HNG58589.1"
                     /translation="MSFRTKNGHEYLTYGQGNGQNHKSLGRRSPDTERQLLAFTQGKE
                     HAGAQLDAIQTRLAERAPQLKAVRLGRILAPLAQVIREFDVHGMIGSKLLVAGTHAIT
                     AYEAMAGHFFDAELTATEDLDLVWRQGKNVEILVKNSESFALLKHLKTVDSSYTVNTE
                     RTFQARNNKGLIIDFISDEEGVANAPKEYLKPIALPGQEWLIKAATLSVVAIDTNGLP
                     VKLAVPDPRVFALHKVWVSQRA"
     gene            complement(945..2105)
                     /locus_tag="PKZ52_02115"
     CDS             complement(945..2105)
                     /locus_tag="PKZ52_02115"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNG58590.1"
                     /translation="MRNETQQLVRWLTEQVLPRYRTHASIPGLPAGMWASFEALKANG
                     EPNPALNLRVRVQARQIFVYCRAQKMGWASDVEPMVRGLAEFIGHYAVTECRSDGYVH
                     LLKPDFSLADSKKDLYDHAFFTLAYGAIYDTFGDKNAHRKARRIIKWVNKEWAAANGG
                     WQEGDYSAPWRRQNPHMHWLETFLFLYEASGKIEWAERAKTVFALFKQHFYEPKAQVL
                     LEFFDSDWSVAPGAIGEAVEPGHMLEWVWLLRWYERLMDEDTSHYANALWHKALAIGL
                     DSEGLVYDQVTADGRVTEATKRLWPMTELIKAGVSQARAGVEGAEAVAAKAIADLQRF
                     YLTTAVPGIYLDKLGAANEVVDDGAPASTLYHLIGAAIEAQNYLNSPASSLS"
     gene            2357..3430
                     /locus_tag="PKZ52_02120"
     CDS             2357..3430
                     /locus_tag="PKZ52_02120"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNG58591.1"
                     /translation="MTSPTVDDLNVNSQEILISPAELKAELPASERAIETVASGRQVV
                     RDILDRKDHRLIVVIGPCSIHDPIAAMDYARRLKTLAAEVSDCLYIVMRVYFEKPRTT
                     VGWKGLINDPYLNDSFKIQDGLHVGRKLLLDISELGLPCATEALDPISPQYLHDLISW
                     SAIGARTTESQTHREMASGLSSAVGFKNGTDGSLTVAINALQSVSSPHRFLGINKEGR
                     VSVVHTRGNAYGHVVLRGGNGKPNYDSVSVALCEKELLAAGVPNNIMVDCSHANSNKS
                     HELQPLVMDNVANQIIEGNTSIIGLMVESNIGAGNQKIPADLSKLTYGVSVTDACIDW
                     PTTESALHSMRSKIKAVMHARGK"
     gene            complement(3499..4350)
                     /locus_tag="PKZ52_02125"
     CDS             complement(3499..4350)
                     /locus_tag="PKZ52_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNG58592.1"
                     /translation="MTSRPLDEPLHFSSQEYDEFCAFLQSSCGIDLGHNKQYLVATRV
                     RRIMVSLQLTKLAQLTELIKKVDQRQVRQSVIDAMTTNETFWFRDTYPFDYFGAKLLP
                     RLQAQKPGSTLKVWSAACSYGQEPYSLSMVVEEFNKRAGGKSLAVDILATDLSSAVLE
                     HARTGVYDRISIARGLTTERKTQFFEAVDEDNWRLKTNVKHRVQFRPLNLQDSFYLLG
                     KFDVIFCRNVLIYFSQELKTEILRKLHGQLHPGGFLFLGSSEGLTGVAELFEMVDCAP
                     GLAYKAI"
     gene            complement(4596..5528)
                     /locus_tag="PKZ52_02130"
     CDS             complement(4596..5528)
                     /locus_tag="PKZ52_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheV"
                     /protein_id="HNG58593.1"
                     /translation="MASILDSVNQRTQLVGQNRLELLLFSLGGNQLYGINVFKVKEVL
                     QCPVLSKIPKRHPVVRGVAHIRGGTIPIMDLRQATGGMPLENAQNCFVIITEYNRSTQ
                     GFLVRSVERIVNMNWGAIHPPPKGAGRDNYLTAVTEVEGRLVEIIDVEKILAEVSPLP
                     EHVSADVIKESESNAVQSRHVLIVDDSAIARKQIQKVVESLGLKTVVLKDGQEALNYL
                     LAMLDEGKDPIKELLLVISDIEMPEMDGYTFTAEVRANPRLSNLHVILHTSLSGVFNE
                     AMVRKVGANDFLAKFHPDELAKRVNVRIKELDGH"
     gene            6420..7148
                     /gene="flgA"
                     /locus_tag="PKZ52_02135"
     CDS             6420..7148
                     /gene="flgA"
                     /locus_tag="PKZ52_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNG58594.1"
                     /translation="MSTCGFARAGLTAFFCLFIHTIAQAQDLFEEIEPLRNKAQQLLI
                     SQLQPTAGTATRDIEVKMLPLDPRLRLSRCDVPVDYQINSPVATATSATVKISCSGTV
                     HWSLYAPAQIAVFGETAVASHSLERGSLIQPGDFHFVRQNLSQIGANYVDPTAVIVGQ
                     ELKRSMREGEPLRLSYLELPKIVQRGDRVTLEAQALGLNVAAPGLAMANGKVGERIQV
                     KNAQSNKIVDALVIAPGRVRVKYE"
     gene            7268..7603
                     /gene="flgM"
                     /locus_tag="PKZ52_02140"
     CDS             7268..7603
                     /gene="flgM"
                     /locus_tag="PKZ52_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNG58595.1"
                     /translation="MVIDPSNSNFGKIPIKKGYSGTVSQERAQATASANNSASAQSNK
                     PTAAAADNVSLSAKAQSLARLESRVAQAPEVDRKKVEAIKQSIAEGRYKVNPEQIAKA
                     MLDAERQGY"
     gene            7874..8359
                     /locus_tag="PKZ52_02145"
     CDS             7874..8359
                     /locus_tag="PKZ52_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNG58596.1"
                     /translation="MQTESLQQIADSIDSDIIACEHLIHLLHHERDLLAERAFEPLDL
                     IIQEKADWLKKLEHSARLRQACLEASGLAQQADPNSAFLHWLTANNQQQLAQRWERLK
                     ELLNQCKEANEVNGKLIHRGQATHQTLVGILRGQDHQTMLYTGKGVKKNTGIAAPIGK
                     A"
     gene            complement(8563..8934)
                     /locus_tag="PKZ52_02150"
     CDS             complement(8563..8934)
                     /locus_tag="PKZ52_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNG58597.1"
                     /translation="MAVFSQFPIAVTVVLLICSNVFMTFAWYGHLKHLQEKPWIIAAL
                     VSWGIALFEYLLQVPANRMGAQVLNLGQLKILQEVITLMVFVPFAVFYMRESFRWDFV
                     WAGLCLLGAVFFIFRERIMGG"
     gene            9242..10693
                     /gene="gnd"
                     /locus_tag="PKZ52_02155"
     CDS             9242..10693
                     /gene="gnd"
                     /locus_tag="PKZ52_02155"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNG58598.1"
                     /translation="MKPLSDIGLVGLAVMGENLILNMASKGFTVTAYNRTADKVQNFL
                     DGRAQGKTIRGAFSIEELVNSLAKPRKIMLMVKAGKPVDDMIEQLIPHLEPGDIIIDG
                     GNTHYPDSTRRTHHLASKGLLYIGTGVSGGEEGALTGPSIMPGGAKAAWPHVKDILQG
                     IAAKVADGTPCCDWVGDDGAGHFVKMVHNGIEYGDMQLICEAYQIMKDLLGMSADEMH
                     QVFKDWNEGELNSYLIEITRDILGYKEDGAALVDSILDTAGQKGTGKWTGVIALDLGV
                     PLTLITEAVFSRCLSAQKDERVAASSLLSGPTPSFTGDKQAFIEDLRQALLAAKIVSY
                     AQGYVLMREAAKDYNWNLNYGGIALMWRGGCIIRSSFLGNIKEAFDANPELTNLMVTP
                     YFRDKLQACQAGWRRVMAAAISQGISAPAMTAALSYFDGYRTARSPANLLQAQRDYFG
                     AHTYERTDKPRGEFFHTNWTGRGGNTASTTYNV"
     gene            10754..11101
                     /locus_tag="PKZ52_02160"
     CDS             10754..11101
                     /locus_tag="PKZ52_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNG58599.1"
                     /translation="MANEHWVCRADDIACGTAKGFCIAEERFFILNQGGEFRAYRNRC
                     PHLNIPLEWQEDSFLCKDTDLVRCATHGALFLPENGLCVSGPCAGQSLEPLTIALRRD
                     GIYWLQAKPTTES"
     gene            complement(11098..11817)
                     /gene="sfsA"
                     /locus_tag="PKZ52_02165"
     CDS             complement(11098..11817)
                     /gene="sfsA"
                     /locus_tag="PKZ52_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNG58600.1"
                     /translation="MILTPPLQQGVFLRRYKRFLADITSASGEPITIHCPNTGSMRGC
                     LEPASPCWYSTSANPKRKLAATWEIATTPLGHLAGINTGRANALVGEAIKAGLIPQLT
                     GWQDLTAEVRYGEENSRADWCLSLAGQRCFVEVKNVTLLEPELAGAEGCGHFPDAVSE
                     RGSKHLRELIRMVEQGERALLVYCVQHSGINAVAPAAHIDPTYAKWCARAKEAGVEFV
                     ALKAQLSATEILLTHQLPVRV"
     gene            12157..12594
                     /gene="dksA"
                     /locus_tag="PKZ52_02170"
     CDS             12157..12594
                     /gene="dksA"
                     /locus_tag="PKZ52_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNG58601.1"
                     /translation="MPELQNGSFSLHGFAPYVEKAGEEYMSAEQKKHFKNLLLAWKRE
                     LMEEVDRTVSHMKDEAANFPDPADRASQEEEFSLELRTRDRERKLIKKIDKTLELLET
                     EEYGYCDTCGVEIGIRRLEVRPTATQCVDCKTLAEIKEKQIGG"
     gene            12733..13614
                     /gene="gluQRS"
                     /locus_tag="PKZ52_02175"
     CDS             12733..13614
                     /gene="gluQRS"
                     /locus_tag="PKZ52_02175"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNG58602.1"
                     /translation="MPPSYLGRFAPSPSGPLHFGSLVCALASYLDAKQHGGQWLLRIE
                     DIDPPREVPGAAESIVRSLERHGLVWDGPVLWQSQRSAHYEAVLEDLRRAGCIYLCSC
                     TRKRLESLGHRYDGYCRHHPASLPAAWRLKVAEDTQIEFTDRHLGQQVEHLGAQGDFI
                     IHRKDGLFAYALAVVVDDWQQGITHVVRGSDLLSATGNQIYLFNLLGAPAPRYAHIPV
                     ITNSSGLKLSKQNHAPALDAATPGRNLWRALWALGAKPPVELAEAAPSQVIDWGQQHW
                     RLSQLPPGLNCPEAQLP"
BASE COUNT         3404 a         3845 c         3417 g         3074 t
ORIGIN      
        1 ccgcgcgttg cgacacccat actttatgca acgcaaatac tcgaggatca ggcacggcta
       61 atttaacggg taagccgttt gtatctatgg caaccacact gagcgttgcc gccttaatta
      121 gccattcttg ccctggcaac gcaatcggtt ttaaatactc tttgggtgcg ttggcaacac
      181 cttcctcgtc actaataaaa tcaattatca agcctttgtt gttgcgcgcc tgaaatgtgc
      241 gttcggtatt aaccgtatag ctgctatcaa ccgtttttaa gtgttttagc agtgcaaaag
      301 attcagagtt tttaaccagt atttctacat ttttaccctg gcgccaaacc aaatctaaat
      361 cttccgtggc agttaattcc gcatcaaaaa agtgacctgc catagcttca taggcagtaa
      421 ttgcgtgggt gcctgccact aacaattttg agccgatcat cccatgcaca tcaaactcgc
      481 ggatcacttg cgccaaaggg gccaaaatac ggccgagcct gaccgctttg agttgtgggg
      541 ccctttctgc taggcgggtt tgaattgcat ccaattgcgc gcccgcatgc tctttgccct
      601 gggtaaacgc gagtaactgt cgttcggtgt caggggatcg cctaccgagc gatttatgat
      661 tttgcccatt accctggccg taggttaaat actcgtgccc gttttttgtt cgaaacgaca
      721 tccaacctgt gaaattcccg gctaattttt tagccgctag ccactgctca tactaattag
      781 cagcatctgc caacaagcga ttttgatcgg cgttaaactc gatgaaccct ctcaccctct
      841 acatttccca cctaattttt atatttttgt gtattaggtg ggaaaccgct cattcgcaag
      901 gatttaaata actcaaaacc ctgtaccacc tccttactgc tgtactaact taaagaagac
      961 gctggtgagt tgaggtaatt ttgcgcttca atcgccgcac caattaaatg atacaaagta
     1021 ctcgccgggg cgccgtcatc aactacctca ttggccgcgc ccaacttatc caaataaata
     1081 ccgggcacgg ctgttgttaa ataaaaacgc tgtaaatctg ctatggcctt ggcggccacc
     1141 gcttcggcgc cttctacacc agcgcgggct tggcttacgc ccgctttaat cagctccgtc
     1201 atgggccaca ggcgtttggt ggcctctgtg actcggccat cagcagtaac ctgatcgtac
     1261 accagcccct cgctatccaa accgattgcc agagccttgt gccagagggc attggcgtaa
     1321 tgggaggtat cttcgtccat caagcgctca taccagcgca gcagccacac ccattcgagc
     1381 atatggccgg gctccaccgc ctcacctatg gcacctggcg ccacgctcca atcgctatca
     1441 aaaaactcca acaacacctg ggcttttggc tcgtaaaaat gttgcttgaa taaggcaaaa
     1501 acagtctttg cccgctcagc ccactcgatt ttgccggagg cttcatagag gaataaaaag
     1561 gtttccaacc agtgcatatg ggggttttgg cggcgccagg gcgcgctgta gtcgccctct
     1621 tgccagccgc cattagcggc agcccattct ttattgaccc acttgataat gcggcgagct
     1681 ttgcggtggg cgtttttatc gccgaaggtg tcgtaaatgg ccccataggc gagggtaaaa
     1741 aaggcgtggt cgtagagatc tttcttgcta tcggccaggc taaagtccgg tttgagcagg
     1801 tgtacatagc cgtcgctgcg gcattcggtc acggcgtaat ggccgataaa ttcagccagg
     1861 ccccgcacca tgggctctac atcgctcgcc caccccattt tttgcgctcg acaatacaca
     1921 aatatctgcc gggcctgtac ccgcactctt aggtttagcg ccgggttagg ttcaccgttg
     1981 gccttgagcg cttcgaaact cgcccacata ccggcgggca acccaggtat agacgcgtgc
     2041 gtgcggtagc gcggcaggac ctgctcggtt aaccagcgca ccagctgttg ggtttcgtta
     2101 cgcataccaa cctcatttat ccagttattt ataatcaacg ccccatgctc tgggtattgg
     2161 cctaggctta agaatcgggc gaggttacaa acttaaaccc cacaggtaaa gtaaatgcta
     2221 tgcccgaaag ccctcattca ccacccaggc cggcaactat ggccgcccgg tggggaacag
     2281 aaaccgccca ttaaggcgag ttttcagtac aatgaaccca catcaccaat gccaatggcc
     2341 agacgagaac atctgcatga ccagcccaac tgttgacgat ctcaacgtaa actcccaaga
     2401 aattctgatt tccccggctg aattaaaagc cgaactgccc gccagcgagc gcgccattga
     2461 aaccgtggcc agcggccgcc aagtggtgcg ggatattctc gatcgcaaag atcaccgctt
     2521 aatcgtcgtg ataggcccct gctccatcca cgaccccatc gccgccatgg actatgcgcg
     2581 ccgcttaaaa accctcgccg cagaagtgag cgactgcctg tacattgtga tgcgcgtgta
     2641 ttttgaaaaa ccccgcacca ccgtgggctg gaagggttta attaacgacc cctacctaaa
     2701 cgattccttc aaaatccaag atggcctgca tgtaggccgc aaactgctac tggatatttc
     2761 cgaactcggc ctgccctgcg ccaccgaagc gctggatcca atctcaccgc agtatttgca
     2821 cgatcttatc tcctggtccg ccattggggc gcgcaccact gaatcacaaa ctcaccggga
     2881 aatggccagc ggcctgtcct ccgccgttgg ctttaaaaac ggcaccgacg gcagcctcac
     2941 tgtcgccatc aatgcattgc aatcggtctc cagcccccac cgctttttag gcatcaataa
     3001 agagggccgc gtctctgtgg tacatacccg cggcaacgcc tatggccatg tggtgctgcg
     3061 cggtggcaac ggcaaaccca attacgattc ggtaagcgta gccctgtgcg aaaaagaatt
     3121 actggcggcg ggtgtgccca ataacatcat ggtggattgc agccacgcca actccaacaa
     3181 aagccacgaa ctgcaaccct tggtaatgga caatgtggcc aaccaaatca tcgagggcaa
     3241 tacctcgatc ataggtttaa tggtggagag caacattggc gctggcaacc agaaaatccc
     3301 ggcggattta tccaaattaa cctatggggt ttccgtcacc gacgcctgta tcgattggcc
     3361 caccacggaa agtgccctgc actccatgcg cagtaaaatt aaagccgtga tgcatgcgcg
     3421 cgggaagtaa cacaaaaacg gggctgcaaa gccccgtttt tgtttgtata gctaactgaa
     3481 gagcagcgca atcagctgct aaattgcttt ataagccagc cccggcgcac aatcgaccat
     3541 ctcaaataat tctgccacgc ccgtgagccc ctctgaggag cctaaaaaca aaaacccacc
     3601 tgggtgcaac tgcccgtgca gcttgcgcaa aatttcggtt ttgagctcct gggaaaaata
     3661 aatcagcacg ttgcggcaga agataacgtc gaacttaccc aacaaataaa aagaatcctg
     3721 caggttgagc ggacgaaact gtacgcggtg ttttacgttg gtttttaatc gccaattgtc
     3781 ctcatccacc gcttcaaaaa attgtgtttt gcgctctgta gtcaatccgc gcgcaataga
     3841 gatgcggtcg taaacacccg tgcgggcgtg ctccaacact gcactggata agtccgttgc
     3901 caaaatatcc accgctagcg atttgccgcc agcgcgctta ttaaattcct ccaccaccat
     3961 actcaacgaa tagggctcct gcccgtaaga gcaggcggca gaccagactt ttagggtgct
     4021 gcctggcttc tgcgcttgca acctgggcag caatttagcg ccgaaatagt cgaacggata
     4081 ggtatcgcga aaccaaaaag tttcattggt ggtcatggcg tcgatgacac tctgccgcac
     4141 ttgccgctga tccacttttt taattaactc cgtcagctgc gcaagcttag ttaattgcaa
     4201 gctcaccata atacgccgca cacgggtggc cactagatat tgtttattgt ggcctaaatc
     4261 aataccgcag gaactctgca aaaatgcaca aaactcgtcg tactcctggg aggaaaaatg
     4321 caaaggctca tcgagcgggc gcgacgtcat aacacacccc acaccgtttt atgcacctgc
     4381 gagcaagccg gtgcacagcc gcgtcccggc ctgccaggcc ctattgccca gaataaatta
     4441 cagccgcttg cgacttcctt taactccgtc attcgcgatc ccgccctcat attggagaaa
     4501 gtttaataac caggacttgc tgccctgttt aacggcctgt tgcaacacca ccttgccagc
     4561 tctggctgca attgcgcgtt gcaataagcg gttaattaat gcccgtctaa ttctttaatg
     4621 cgcacattca cacgcttggc taattcatct ggatggaatt tcgcgagaaa atcattggcc
     4681 cccactttgc gcaccatggc ctcattaaat acaccactca aagacgtgtg caatatcaca
     4741 tgtaaattag ataagcgcgg attggcgcgc acctcagcgg taaaggtata accgtccatc
     4801 tccggcattt cgatatcaga aataaccagc agcaattctt tgataggatc cttgccctca
     4861 tccaacatcg ccagcaaata attgagcgcc tcttggccat cttttaacac tacggtttta
     4921 agacctaagg attccaccac tttttgaatt tgtttacggg caatggccga gtcatcgaca
     4981 attaatacat ggcgactttg caccgcgttg ctttcgcttt ctttaatgac atcggcactg
     5041 acgtgctccg gtaatggcga tacttcagcg aggatttttt ccacgtcaat gatttccacc
     5101 aagcggcctt ccacttcggt gacggccgtc aaataattat cacggccagc gcctttgggc
     5161 ggcggatgaa ttgcgcccca attcatattg acgatgcgct ccacgctgcg cactaaaaac
     5221 ccctgggtag agcggttgta ctcggtaata ataacaaaac aattttgcgc gttttccagc
     5281 ggcatgccgc cggtagcctg gcgcaaatcc atgatcggaa tggtgccgcc gcgaatatgc
     5341 gccacaccgc gcaccacagg atggcgcttg ggaattttgc tcagcaccgg gcactgcaac
     5401 acttctttca ctttaaacac attaatgccg tagagctggt tgccgccgag gctgaacaac
     5461 agcaattcca accggttttg accgactaat tgcgtgcgct gatttacgct atcgagaata
     5521 ctcgccataa ccgctccaag cgattgcaca gaaaatattg caacggtatc caagcagaat
     5581 taaggctact ggcagcccac cctaactgtt aaaccgtctc cgctgctcta ttgatgttta
     5641 tcccatagat agtactaaag ggcacctccg gaaattcact tttcgcgtcc tcgctgtgtt
     5701 gttttcgtgc tcaatcggtc atttatatcg cataaactcc ctcatgccgc gcgaaaatac
     5761 cttgcgatca ctgcgaaaag tgaatttcga aggccccctt aaggaaaatc tgaatgatta
     5821 atccagacgc tcctgtatgg ctttaacaca tcaaagaggc acctaactgc ctaagtgtag
     5881 tcaagactca tggccacaca gaaaaacaat ggccaattct gcaaacaaaa gctcgtagca
     5941 taaaacggca aattgtccac tgaataaccg agctgacaga gacaagcttg catgtcatgg
     6001 cgcaggcaca accatttaac ctaaaaattc agttaaaccc gtggcttggt tttgcgagcg
     6061 aattggcgca aaaagggtgt cgaataaaaa atctaaccgg tacactgcac cctagcagcc
     6121 tggtgaaatg cctagggaac ctctgaaaaa ttaccttact cgccctggcg tcgttgaatt
     6181 tgccaaaaaa atgctcattt actgcgtgta aactccgctt tttttggcaa attcgcctag
     6241 ccagcgcttc gccgcctaca tttttcagag gttccctagc aaggtagtat tgcaacagac
     6301 tgctagcatg caaaaggcac agcaattgcg taatccaatg gatacctaca ataacgacaa
     6361 aaatccgtca tctacgccca gtagatttgt cttgccaacc taagcacagg ttaacaatta
     6421 tgagcacatg tggttttgcc cgcgcaggtc ttacggcatt tttctgcctc ttcatacaca
     6481 ctatcgccca ggctcaagac ttatttgaag aaatagagcc gcttcgcaac aaggcgcaac
     6541 agctgttaat aagtcaatta cagccaacag cgggtaccgc cacgcgggat atcgaagtca
     6601 aaatgttgcc gctggatccg cgcctgcgtt tgagccgctg cgatgtgcca gtggactacc
     6661 aaatcaattc ccccgttgcc accgccacct cggcgacggt caaaataagc tgcagcggca
     6721 cggtgcattg gagcctttat gcgcccgcgc aaatcgccgt cttcggtgaa accgccgtgg
     6781 cctcccacag cctggagcgc ggcagcctca tccagcccgg cgatttccat ttcgtgcggc
     6841 aaaacctcag ccaaattggc gccaactatg tggacccaac tgcagttatt gtcggccagg
     6901 agttaaaacg ctccatgcgg gagggtgaac ccttgcggct gtcctatctg gagctgccta
     6961 aaatcgtcca acggggcgat agggttacac ttgaggccca ggccctgggc ttaaacgtag
     7021 cggcaccggg actggccatg gccaatggca aggtgggcga gcgcattcag gttaaaaatg
     7081 cccaatccaa taagattgtg gatgccctgg taatcgctcc gggtcgtgtg cgggttaaat
     7141 acgaataata gcgatggcaa gcggggcttt tgcgggtatg aagatttttt gctatcggcc
     7201 ctaaaggatc tacccagatg gccgataacc cgatgaacct acacgaatac gctgcggcga
     7261 tatgattatg gtaattgatc ctagcaactc aaatttcggc aaaatcccga tcaaaaaggg
     7321 atattccggc actgttagcc aggagcgtgc acaggctacg gcaagcgcta ataacagcgc
     7381 ttcggcacag agtaacaaac ccacagctgc ggctgcggac aatgtctcct taagtgccaa
     7441 agcccaatcc ctcgcgcgct tggaaagccg ggtcgcccag gcgcctgagg tggaccgcaa
     7501 aaaggtggaa gcaattaaac aatccatcgc tgaaggccgc tataaagtaa accccgagca
     7561 gatcgccaaa gccatgctag acgccgagcg gcagggttat taggcggcgg actcttttgg
     7621 tggcggggca ccatgccctg cctgccccct caaattaggt tttaaaaacg ccgcaacagc
     7681 tttatagggc tcaacacttt ttggcgccca gttttttaag cctagctata cttgagtgca
     7741 gcttcacttt tttgcgtttt tgcgcgtccg gatgtatttg ccatttacta ccgcttaaca
     7801 gccgtaggct ggattggcgc gcattgacat cagtactggg tataaaaaag ctaatcacca
     7861 ctcgggcaca gctatgcaaa cagaatccct tcagcagatt gccgactcca tcgacagcga
     7921 cataatcgcc tgcgaacatt taattcatct actgcatcac gagcgggatt tattggcgga
     7981 acgagccttt gagccgttgg atttaattat ccaagaaaag gccgactggc tgaaaaaatt
     8041 ggagcattcc gcaaggctca ggcaagcctg cctagaggcc agcggccttg cccaacaggc
     8101 cgatccgaac agcgcgtttc tccactggct cacggcaaat aatcagcaac agctggccca
     8161 gcgctgggag cggttaaagg aattactcaa ccagtgtaaa gaggccaatg aggttaacgg
     8221 caagcttatt caccgcggcc aggctacaca ccaaactcta gtgggtattt tgcgcgggca
     8281 ggatcatcaa accatgctct ataccggcaa gggcgttaaa aaaaataccg ggatagcagc
     8341 gcccattggt aaagcataag ttaatgcatc aatgacctga cttgcgcaca cgaaaatgcc
     8401 ccctggtgaa aaacgaagtt gacaccaatt gggctcagta gctctgatgc ccccgagtaa
     8461 ttacttctgc aacttacccg ccgctgggcg gcaaaaaatt aaatcctctg atgattaaat
     8521 gattgcctat ccaatgcaaa tttctccgca ggacaaacag gtttaaccac ccataatgcg
     8581 ctcgcgaaaa ataaaaaata ccgcaccgag cagacataac cccgcccaga cgaaatccca
     8641 gcggaaactc tcgcgcatat aaaaaacagc gaagggcaca aataccatca gcgtgataac
     8701 ttcctgcaga atttttaatt ggccgagatt taatacctgg gcgcccatgc ggttggcggg
     8761 cacttgcaat aaatattcaa acaaagcgat gccccagctc accagcgcag caataatcca
     8821 gggtttttcc tgcagatgtt ttaagtgccc ataccaggca aaggtcataa aaacattgct
     8881 gcaaatcaat aatacgacag taactgctat gggaaattgg gaaaaaacag ccataaaaaa
     8941 ctcattgatg atggtggatt tttacgccgc taagacagcg ctggcgcagc gctaaatagc
     9001 cgagggcggc agcgattttg cgcaggccag aagggctttt ggcgcacttt ataaagggct
     9061 ttttgattcc ctaagcgggg gataagtggt atggtacccg cccttgtcgc agggctaaag
     9121 cgcaccgcat tgcttcactt taacgctaag caaaatgggc actttgttac ataccctcca
     9181 gcaaaattcg cggcagtgtg cgctgccaat tcatatcact tatacgatcc atgtgtagcc
     9241 tatgaaacct ctttccgata taggccttgt cggcctcgcc gtgatgggcg aaaacctcat
     9301 tcttaatatg gccagcaaag gctttactgt gacggcctat aaccgcacgg ccgataaagt
     9361 acaaaacttc ctcgacggcc gcgcccaggg caaaaccatt cgcggcgcct tttccatcga
     9421 agaactcgtc aactccctgg ccaagccgcg caagattatg ttgatggtaa aagcgggcaa
     9481 gccggtggat gacatgatcg agcagctaat cccgcatctt gaacccggcg acatcattat
     9541 cgatggcggc aacacccact acccagattc cactcgccgc acccaccacc tggcgtccaa
     9601 gggcctgctg tacattggta ccggtgtatc cggcggtgaa gaaggcgctt taactggccc
     9661 ctccatcatg cccggcggcg ccaaggcggc ttggccccat gtgaaagata ttctgcaagg
     9721 cattgcggct aaggtcgccg acggcacccc ctgttgcgat tgggtgggcg atgacggcgc
     9781 aggccacttt gtgaaaatgg tgcacaacgg catcgaatac ggcgatatgc agttaatctg
     9841 cgaagcctac caaatcatga aagacctgct ggggatgagc gccgatgaaa tgcaccaggt
     9901 atttaaagac tggaacgaag gcgagctaaa cagctattta attgaaatta cccgcgacat
     9961 cctcggctac aaagaagacg gtgctgccct ggtagatagt atccttgata ccgcagggca
    10021 aaaaggtacc ggcaaatgga ctggggtgat cgcccttgac ttgggtgtac ccctgacgct
    10081 gattaccgaa gcggtcttct cccgctgttt atccgcccag aaagacgagc gcgtggccgc
    10141 cagcagtctc ctgagcggcc caacgccgag ctttacgggc gacaaacaag cctttattga
    10201 agatctgcgc caggccttgc tcgccgccaa aattgtctcc tacgcccagg gttatgttct
    10261 gatgcgcgaa gcggctaagg attacaactg gaacctcaac tacggcggca tagccttgat
    10321 gtggcgcggc ggctgtatca tccgctcatc cttcctaggc aatattaaag aagcctttga
    10381 tgccaacccg gagctgacca acctgatggt cacgccttac ttccgcgata agctgcaggc
    10441 ctgccaagcg ggctggcgcc gggtgatggc ggcggctatt agccagggca tcagcgcccc
    10501 agcaatgaca gcggccttaa gttacttcga cggctaccgc accgcccgct cccctgccaa
    10561 cctgctgcag gcgcagcggg attatttcgg cgcccatacc tatgagcgca ccgataaacc
    10621 ccgcggcgag ttcttccaca ccaattggac cgggcgcggc ggcaacacgg cctccactac
    10681 ctacaatgtg taaaccctaa agccccaccc ggggcttttt tttcgagcag gttttcagta
    10741 attgaaggtg tatatggcca acgagcactg ggtatgccgg gcagacgata tcgcctgcgg
    10801 tactgcaaaa ggtttttgta ttgcggagga gcgttttttt atcctcaatc agggcggcga
    10861 gtttcgcgcc taccgcaacc gctgcccgca cttaaacatc cccttggaat ggcaggaaga
    10921 cagtttcctg tgtaaagaca cggacctggt gcgctgcgcc acacacggcg cgttgtttct
    10981 gcccgaaaat ggcctctgcg taagcggccc ctgcgccggg caatccttgg agcctctgac
    11041 tattgctctg cgccgggacg gcatttattg gctgcaggct aagcccacaa ccgagtctta
    11101 aacccgcaca ggcaattggt gggtaaggag aatttccgtg gccgaaagct gagctttcag
    11161 ggcaacaaat tccacccccg cttcttttgc gcgcgcacac cacttggcgt aggtggggtc
    11221 tatgtgagcg gcgggggcca cggcgttgat gcccgaatgc tgtacacaat acaccagcag
    11281 ggcgcgctca ccctgctcca ccatgcggat cagctcccgc aggtgtttac tgccgcgctc
    11341 gctcactgca tcggggaagt gaccgcagcc ctcggcgccg gctaattctg gttccagtaa
    11401 agtgacgttt ttaacctcca caaaacagcg ctggcctgca aggcttaagc accagtccgc
    11461 ccggctgttc tcctcgccgt agcgcacttc ggcggtgaga tcctgccagc cagttaactg
    11521 cggtattagt ccggctttaa ttgcctcccc caccaaggcg ttggctcggc ctgtattaat
    11581 gcccgccaaa tggcccaaag gcgtggtggc gatctcccag gtggcggcga gtttgcgttt
    11641 ggggttggca cttgtggaat accaacaggg gctagcgggt tccaaacagc cgcgcatgct
    11701 gccggtattg gggcagtgaa tggtgatggg ctcgccgctt gcagaggtaa tatcggctaa
    11761 aaaacgctta tagcgccgca aaaatacacc ttgctgtaaa ggaggggtca atatcatggt
    11821 gacaatctta agcttttgac tatgctgatc caccaggaat ccctggcggg aagtgggggt
    11881 tagggggttc actcaacgcc ctatgatacc ccagcgcccc cgtcaaaatg cctgcgcggc
    11941 ggttttccgt ggaggcaaca gaagtggtgg cagaggctta gggaatctgg tagcgtttgc
    12001 gccccttaga taaccatgaa tctaaacgcc gcagttaacc tccgctgggg ttattaggtt
    12061 gaaaaagacc gggccgacct catattgggg ccgtccttac ggaaagtatt gtccccaagg
    12121 atcgctttca tcgcacagac aagaggctcg ctcacaatgc ccgaattaca aaacggtagt
    12181 ttttcgttac acggttttgc cccttatgtt gaaaaggctg gcgaagagta catgagtgcc
    12241 gaacagaaaa agcactttaa gaatttgctg ctggcgtgga agcgtgagct gatggaagag
    12301 gtggaccgca ccgtaagcca tatgaaagac gaggccgcca acttcccgga cccggccgac
    12361 cgcgccagcc aagaagaaga atttagcctc gaactgcgca cccgcgatcg cgagcgcaag
    12421 ctgattaaaa aaatcgataa aaccctggaa ttgttggaaa ccgaagagta cggctactgc
    12481 gatacctgcg gggtggagat cggtattcgc cgcctagaag tgcgccccac cgcgacccag
    12541 tgtgtggatt gcaaaaccct ggcggaaatt aaagaaaagc agatcggcgg ttaaacaccg
    12601 gtttgcaggc ataggtgaat cccgctgtgc aatacaacat aaaagagggc catgagccct
    12661 tttttgttgt gcccgcaaac tgcctctgtg tctacaacct gtaactctgc ctgtaacagg
    12721 cccgccacgg ttatgccccc atcgtaccta ggccgcttcg ccccctcccc ctccggcccc
    12781 ctgcattttg gctccctggt gtgcgccctc gcctcttacc tggacgccaa acaacacggc
    12841 ggccaatggc tgctgcggat tgaagatata gacccgccga gagaagtgcc gggagcggcg
    12901 gagagtattg tgcgcagctt ggagcgccac ggccttgttt gggatgggcc agtgctctgg
    12961 cagagccaaa ggagcgccca ctatgaagcg gtgctggagg atttgcgcag ggccggttgc
    13021 atctacctgt gcagctgtac ccgcaaacgc ttggaatcct taggccaccg ctacgatggt
    13081 tattgccgcc accacccggc aagcttgcct gccgcctggc gcttaaaagt tgctgaggat
    13141 acccagattg aattcaccga ccggcatttg ggccagcagg tggagcattt gggcgcccag
    13201 ggggatttta ttattcaccg caaagacggc ctatttgcct atgccctggc ggtggtagtg
    13261 gacgactggc agcagggcat cacccatgtg gtgcgtggca gcgatttact tagcgccacg
    13321 ggcaatcaaa tttatctgtt caacctgtta ggtgcgccag caccgaggta cgcccatatt
    13381 cccgtcatta ccaattcatc aggcttaaag ctcagcaaac aaaaccacgc cccggctttg
    13441 gatgcggcaa cgccggggcg caacctgtgg cgggctttat gggccttagg tgccaagccg
    13501 cctgtggagt tggcagaggc ggcgccgagc caggtgatcg actgggggca acaacactgg
    13561 cgtttaagcc agttaccgcc gggcttaaat tgccccgagg cacagctgcc atagcacact
    13621 ctgtaagttg agaatttttg gagccagcac ggcctaaaat cctctcctgt gacggcaatg
    13681 tcattaagtt aatgatttgc cagcttgcaa acagccttag gggcgcccct tgtgggtgcc
//
LOCUS       DAOQGS010000032        13653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2090_length_13653_cov_4.807251, whole genome shotgun sequence.
ACCESSION   DAOQGS010000032 DAOQGS010000000
VERSION     DAOQGS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2090_length_13653_cov_4.807251"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKZ52_02180"
     CDS             complement(<1..994)
                     /locus_tag="PKZ52_02180"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58603.1"
                     /translation="MHTPFTTPQQRRTWPALLGLLLLSAGLAQGALAANVRVMKQGIG
                     SGTVSSTTAGINCGADCDEAYGGGLTITLTATPAAGSMFAGWEGDCAGLGSCVVNTNL
                     ARSVRAQFVPITPIPTLASFTPEGIQTFLTDNPSITTPAQFISALPAEFKQNWLLMSR
                     SESLQTGTAAMPRILMPSANTQFVFSLGLATHSAYPGAHPNAIEYMQWDPAEKNFRFH
                     EIVLAPVGAMGPVPARSRGVNIDDDKCTRCHSTRNVLNFTMDPGTTGDPVGLVKAKNK
                     PNWDTYDSWAGLMPFNRDRIYQGSVEAAAFRKIFNMWNWRSNEPVRAVLEQLQLQ"
     gene            1617..2054
                     /locus_tag="PKZ52_02185"
     CDS             1617..2054
                     /locus_tag="PKZ52_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG58604.1"
                     /translation="MSTSLIMRQFIRHPISIPIQVHPIDHPAASVAVQNLSAGGLCFV
                     TDTPVAVGSMVEFVIPNLEPDYHGDGIVVWRKDTAPNLFSVGMCFITDDEYYRTRMVE
                     QVCRIEAYRRELAEQGRQLTPEAAAEEWIARFAAKFDDSHGIN"
     gene            3182..7021
                     /locus_tag="PKZ52_02190"
     CDS             3182..7021
                     /locus_tag="PKZ52_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM system kinase/cyclase fusion protein"
                     /protein_id="HNG58605.1"
                     /translation="MPHLQTNSALINSHSILGYTIVDCLGSGGFGQVFKAIKLNTQQT
                     VAIKVLNLGPQLSDEARKRQVARFNRETHLCALLDHPHIVRLLDRGQTAEEHLFAVFE
                     YVEGQTLQDYLKSGGLPAQEAADIMLQVLDALAHAHQKGIIHRDIKPANIMLTRIGKG
                     IFAKVLDFGIGTFAQGVLPLEHTQLTLTLETLGTPAYSAPEQLRGERATPKTDLYLWG
                     LVFLECLTGKPAVNGSSIASLLHKQLNDLALPIPQYIAVHPLGGIFRRVLSKKTHTRA
                     GNAAELYQEVKTINFAALVSPPAQTAPVHLALEALETPTVIDPWGPAITGQRQRKQIT
                     VLALRFELGAQILDQDVEIIDTLLRDQKYQCTDIIERYCGFFSGSLSNLVLAYFGHPQ
                     ATDNDARLCARAALDIVGHIRQCQLPGANIRAHIGIHTGLVISTNQEAELDGICVDTA
                     VQLSFAAPANSIQCSHATYQMLSMHLDFTHQNQCYFLSGERQVEAFGFLRSHQSSHEL
                     VGRKEELAHVQHFLAQPTPSYLHIHGEAGIGKSRLVFEVRSNAQAYQHYIAQCLPEHQ
                     YSGLYPILRLVQLESQILNTTAAQAVQQLRARLERMNLPANALTLLCIWLNLPLPTEQ
                     PASIEALEEQKQTLFKTLYALLHGSATEPTAKLFIIEDLHWADPLTLEFLAYSHSQSG
                     SLASPHKWIGTSRTAFPIEAACSQRQTLAINKLTPHQIQLLVSILLERQTLSLRLIDL
                     IIERTDGVPLYVEELTRMLERKGLIYRLNGIVDLTSPQAIEQVPSSLKDSLQQKLDYL
                     SMSKETAQLAAVIGRTFGYTLLLQASPHPEEKVQQDLKELISQDLIVLQRHVHGDRYI
                     FKHALVRDAAYESIPSPQRKLLHHKVAQCLEASEESIDNTILAQHWAKAGDFVKASHY
                     TIAQANEALKRSSASEAIVLAQQLEQWAANIPDATRSEHQLATYRLFTTAYMESKGWA
                     SDEVKHYTEASIAILKQQNKAEEIVPQLWWKVLGGVVAGARAPLAPTIAEMDLIFDSV
                     NAINKSAIRCAQGFYYFAEGRRDKCISCFKDSIACYDSSIDIAHQQTFGFDVAVFAKA
                     TLGRTYVDMNEQALAIQNVTQAVQEARSHNNIPSLGIALMYYAIVFQQYGNKPQVQQA
                     AGELLALAEKHDMVVYHLYGQMLYDWAVGTTENAAEILQKLTEMGSFFAVGQFQSFYA
                     DTLIENQYYKEAIFLIERCLTINNEHNEHFYTPHLLSRQLYCLAATDAGSKQIQLVET
                     ALTERLKETPVPFLNLQ"
     gene            7040..8128
                     /locus_tag="PKZ52_02195"
     CDS             7040..8128
                     /locus_tag="PKZ52_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMA_0021/BMA_0022 family TOMM bacteriocin"
                     /protein_id="HNG58606.1"
                     /translation="MAHQNTLMTVRTNVLRAIAFAWENKDDLENGKFYGKVGLDYMLS
                     KAKASPIGLTSLENMESVNIQFMKPKDTLKYNPGSEGYSKDWIKEPNQEIITICLPSC
                     PTKQNGDTFTPLEKASALMKYYELSPHFFHESSFANNNSISVVPKLALNVNTGLSALN
                     FFPGTHHALLEKEQILRGLQAIKFANIEQFEKDINYGNEIINALQAEENREKLATIQK
                     ELARNRNKDKLGLSEDGFLSFGSIVLQIIAESWKNKDFQNKYDFDMHQNEIEKYDNEV
                     NTLLTKEFQFESLWDMKIRFVFTKEGEFKDSIDRSSFGLSNDRARINGVRTLIEVVLP
                     PCPDNDDDILPALAAYNCIGPRYPFTCS"
     gene            8163..10202
                     /locus_tag="PKZ52_02200"
     CDS             8163..10202
                     /locus_tag="PKZ52_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HNG58607.1"
                     /translation="MSAHCTPYWNPSFICEPHNEAWVLISEGNHYSINGQEAQILDDL
                     IQNNGQLYHYLFAQPDTLTQVKRLRFWDYVRQLNLVLECSPTQPQATNSAGAILPLLN
                     TQGFLPYVVLDPISDPDWLTYAPQAPWVLIKPFGAQPSIGPIFTGEGPCPQCLHTRLL
                     NNQPARRWQQFKRGLTLPPAVPMQDQTPHLAKIADFFNQYLAAAARNPAHAHCMWVLN
                     KDLNAYTEHRVATLPQCPRCGDPHLTQHTFTAPINLTPVFANADLDGGYRTQNREDLL
                     QELKHCISPVTGVLTELTHISAEKAEQLMIYQAAYSTQVVEENFVQLSLGKGVANTQA
                     QLSALGEAFERKAAQYQGDEPLRYALPQELPGKAYLPQELAAFSSAQYQFFAQWEQAN
                     LQQPQWVKPYAADTPIYWCPAWNLTRHRPVYFPAAFCLADTPFDDAHYSIYTHNGNSA
                     GASIEEAILQGALELIERDAAAIWWYNRIPRPQIALEVIAPAQQKIIANTLGNEWDYW
                     LSDISHDLPAVSCVAVGQHKIDGRYVLGFGCHLEVSIAAQRALTELYQLICIKDKVSG
                     PFDFKAIKPHPFLYPRANTAIKYSGDYCARQNVSLHSHIIDLIEALQANQLDLCVVNY
                     SRADVPLKTLKVVAPGLCHFWPQLATPRLYQVAVKLGWLSHPLTEAELNPQALYL"
     gene            10212..11267
                     /locus_tag="PKZ52_02205"
     CDS             10212..11267
                     /locus_tag="PKZ52_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNG58608.1"
                     /translation="MILSAAPATSRHSIKHILLCGSLYGRVYLDALYKTRHLRLAAIL
                     ARGSRTSTLLAEQAGVASITYLDQLATTPDAAIVAVGSEAGFHLAQACLKRRIPVLLE
                     HPVLPSHCSDLLALAQAMDVRLHINSHFSLLPPAQEFIQLCRALNSQAQPLSVQISCN
                     SRTLFSLLDIAQAIFGLFNFEALCSDALDAEEHYYQMRFKLNKIPCIVHYQNWRSAQD
                     DSLDSPLGHQCTITYPQGIMQLNSSFGPCLWFPLMAATTGVAYPAYSSLSRLDQAPTH
                     ADIIAWREDANIQAIESLITPDEDQFTQTPVYLTHLCQNWHTLFTAHGFKVAPPQVNK
                     LTDIFFTAKQLVCSAKR"
     gene            11273..12226
                     /locus_tag="PKZ52_02210"
     CDS             11273..12226
                     /locus_tag="PKZ52_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG58609.1"
                     /translation="MATLINSITGATIKLLPVHLFGRLAETCHTVFDNHYISRVHATI
                     SWTGCTWILRDSSINGTYIDRVLAAGTTQHVLALNSQLQFGNLDADPWQVACLAPPVT
                     MLVPLHPNLPYIELDKIIALPSEQEPAVVISEIAPGDWLCESEMGQALLKSGDLVGSA
                     NHIWRLVIANPFQDTIVDPWPIEDACTEVRLHFHASQNEEHVQLKIHIGQTIIDLQER
                     THHYVLLLLARQYTQDTEAQRIETERGWIDKEQLCRQMGVDLNHLNILIFRFRKQLMN
                     ALPPHVQPPSIVQTRVGQVRIQCAEISITGGLPTHTQHQSS"
     gene            12485..>13653
                     /locus_tag="PKZ52_02215"
     CDS             12485..>13653
                     /locus_tag="PKZ52_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58610.1"
                     /translation="MKHKVQNLLLFFALSQAQTWADGTPPSQPKCIKPERPALPSLND
                     KDLNDSIKLANWVKTASGQYNQYLVTTKEGKSISCANLAAELSNTTLELGSEINSTKN
                     KKLTALNTFADTCSQELAYLLAQEKASLGPDDCKIEVVNSDIDKLNSAQVQLKAEQGL
                     LSQSVDPDQPVKNFSKEQLQSLLNGDKFADIATEKPYYGVLEIGASLMPKYNKEGNNE
                     GFQDANYYAALKLNNRWALKDTKTPYLNWFKPSWVAYQEMNVLFYSAPIACEKNSEPA
                     TNDSPDDPALKSIEESQGSCNKATDIGALKFKDVSHTVNASANFSFLYANKDNIGDWE
                     IGFNARVGLLNREKLSPDLDSVSTYSSGGVEFRLNDFLGKNIAGKTYRNGLPRFV"
BASE COUNT         3722 a         3406 c         3129 g         3396 t
ORIGIN      
        1 gctgcagttg taactgttca agtacggccc gtaccggttc attactgcgc cagttccaca
       61 tattaaaaat tttgcggaag gcagctgcct ccaccgagcc ttggtaaatg cggtccctat
      121 taaagggcat gaggcccgcc cagctatcgt aggtgtccca gttgggtttg tttttggcct
      181 tcaccaggcc caccgggtcc ccggtggtgc ctgggtccat ggtgaaattc aacacattgc
      241 gggtggagtg gcagcgggtg catttgtcat cgtcgatatt gaccccccgg ctgcgggcgg
      301 gcaccggccc cattgcgccc acaggcgcga gaacaatttc atggaagcgg aagtttttct
      361 ccgctgggtc ccactgcatg tattcaatgg cattggggtg ggcgcctgga taggcgctgt
      421 gggtggcaag gcccaggcta aatacaaact gggtattggc gctcggcatc aaaatacgcg
      481 gcatggcggc ggtgccggtt tgcaggcttt ccgagcggga catcaacagc cagttctgtt
      541 tgaactcagc gggcaaggcg ctgataaatt gcgccggggt ggtaatgctg gggttatcgg
      601 taaggaaggt ttgaatccct tcgggtgtaa aacttgccag ggtgggaatg ggggtaatgg
      661 gcacaaactg cgcccgcacc gagcgggcaa ggttggtgtt aaccacacag ctgccaagcc
      721 ctgcgcaatc cccctcccaa ccggcaaaca tagagcctgc tgccggggtg gcagtgaggg
      781 tgatagtaag ccctccgcca taggcttcgt cgcaatcggc gccgcaattg ataccggcgg
      841 tggtggagct gaccgtaccg ctgccgatgc cttgtttcat cacccgcaca ttggcggcta
      901 gggcgccctg agcgaggccg gcgcttaaca gcaggaggcc caacagtgca ggccaggtgc
      961 gccgctgttg gggggtagta aatggtgtgt gcatattcct tctccaagag cttaaaaatt
     1021 gaatgggctc gccacttatt aaatgcagat gtttaaggcg agcacgcagc tgtgtgcacc
     1081 cagtgccgcc aagggcgggc ttgtgatgct cgttgatagt gagctgctgg cgtaacagac
     1141 gctgtggaga aatagcgttg gtatttgtta actattcaca tggagtaaat ggcagggaac
     1201 tttttggcaa ttaatcggcg cgggtttttt cactttgtga atatgatttt tgtgtgtggg
     1261 ttctattcac ctgtgcggca tccttgctgc gcttattgaa ccaacgtcat tacattaaga
     1321 attggggcgc aaatgtgggg cggaattatc tggttggccc ggatgaagcg cgaagcgtaa
     1381 tgctgagtcg ctttgaaatc ccggattacg ctacgctaca tccaggctac aaaaatcgca
     1441 ggtaattctt aacttaatga cattacctgt cggcggcgca taaaaagctc acccagcata
     1501 aaagctgcct gataataaat agagcgattg gtgctactta tcgtgtttag taatagcaat
     1561 acaatttcat ccgcgcacag ttgcctgcat ataattaaat ctcggcagga cacgtcttga
     1621 gcacttcact aattatgcgc cagtttattc gccaccccat ttcgatcccc atccaagttc
     1681 accctattga tcatcctgcg gccagcgtcg cggtgcaaaa cctaagcgcg ggcggtttgt
     1741 gttttgttac cgatacaccg gtggcggtgg gctcgatggt ggagtttgtc attcccaatc
     1801 tcgagccgga ttatcatggc gatggcatag tggtgtggcg caaagatacg gcgccgaatt
     1861 tattttcagt gggtatgtgt tttatcaccg atgatgaata ttaccgcacc cgcatggtag
     1921 agcaggtgtg ccgcattgaa gcctaccggc gggagcttgc cgaacagggc cgccaattaa
     1981 ccccagaggc cgccgccgaa gaatggattg cgcgctttgc ggctaagttt gatgattccc
     2041 acgggattaa ttaagtgcag gattattgcc gcattggtgc cgtacacatt ttgcgggttg
     2101 ttaaatctaa aaaccttagt agagttcaaa tgcggttctt aggttaaaag taagggcacc
     2161 ttcggagctt caattttcgc accctcgcgg cgtcgttttc gcgctcaatc ggtcactttt
     2221 accacataaa ctccctcatt cggctttggc attctgcgtc gctctacctc ctacatccat
     2281 gcagtcgtcc gcgcaaaaat tccttgcgat tatcacgaaa attaaatttc cggaggtgcc
     2341 cgtaagtaac ttattcagca cccttttatt gtggctggac gtgactcccg tgccgaatag
     2401 aaatcaacag gcgaatgcgt gagtgctgtg agccctgtga gccctcagta ctccccttca
     2461 gtggcgaata caggttgttc agacacgccg tgaatacatc cgtgtagact agtcaaaaag
     2521 gtccctcttt ttgacggact gaaataacct gtagtcaccc ctactgcacc gcgaatcgat
     2581 tctgtcgttt ggctaaatac acctaaattt gccgtccacg cttcagaggg gaataaggtg
     2641 tgctcaaaag ctacaaaagt aattgcgtgt tgcaaatgcg atgctgtggg gttttattaa
     2701 cccggcggca aaatagatag ccctgatctg ttttcccttc gctgtgctga cacttgcgaa
     2761 catgcgtgga tattcttttt taagcgcagc tcgcggcggc ttgcgccgca ggcgcggtat
     2821 ccttggccct tttattaacc cggcataaaa agtcgccggg ttaatgctgc cggtgtaatg
     2881 gactgtaatg tatctttaac tcgctagagt ttacagtgca ttccatctgc gaaagctatt
     2941 gcggcaccat ggctcaccac ttgcataaag agctgatgca cttgtgctgc tgtacggcaa
     3001 gagtgcattg atggcgttaa cataagtttg cctcaaacac ggcaattcac tgcctgccaa
     3061 acaagtcctt cacataagtt gttatttgga cataactagc tccctcggct ttgagccggc
     3121 aggtatagca tccgcgcaaa ccaggccaaa acaggtgtta attaaataaa cgggatcctc
     3181 tatgccacac ctacaaacca atagtgcctt aattaattca cactccatat tgggctacac
     3241 catcgtagac tgcttaggca gtggtggttt tggccaggtg tttaaagcga ttaaactcaa
     3301 tacccagcaa accgttgcga taaaagtatt aaacctgggc ccgcaattga gcgacgaagc
     3361 gcgcaagcga caggtggccc gctttaatcg ggaaacccat ttgtgcgcac ttttggacca
     3421 tccacatata gtgcgcctac tggacagagg ccaaactgct gaggaacacc tatttgccgt
     3481 atttgaatat gttgaagggc aaacgctgca ggattattta aagtccggtg gcctgccagc
     3541 acaagaagcg gcagatatta tgttgcaagt gctggatgca ctggcacacg cacaccaaaa
     3601 aggtattatt catcgcgata tcaaacccgc caatattatg cttacacgca tcggcaaagg
     3661 catctttgcc aaggttttgg attttggcat aggcacgttt gcacaaggcg tgctgccgct
     3721 tgaacatacc cagctaacac taacattaga gaccctgggc acaccggcct acagcgcccc
     3781 cgaacagttg cgcggggagc gcgccacgcc caagaccgac ctgtatttat gggggctggt
     3841 gtttcttgaa tgcttaaccg gcaagcctgc cgtaaacggc agcagcatcg cctcgctact
     3901 gcataaacaa ttaaatgacc ttgcgctgcc cattccacaa tatattgcag tgcatccgct
     3961 gggcggaatt ttcaggcggg tattgagtaa aaaaactcac acgcgagcgg gcaatgccgc
     4021 cgaactgtat caggaagtta aaaccattaa ttttgccgca ctggtatcgc cgccagcgca
     4081 aaccgcccca gtgcatttag cacttgaagc actagaaact cccaccgtta tagacccctg
     4141 gggcccggcc attacaggcc aaagacaacg caaacaaatc acggtacttg cgctgcgttt
     4201 tgagttaggt gcacaaattc tcgatcaaga cgtggaaatt atcgatactt tgctgcgcga
     4261 tcagaaatac caatgtacgg atattattga acgctattgt ggtttcttct ccggttcact
     4321 gagcaaccta gtgttagcct attttggtca tcctcaagcg acggataacg acgcccgttt
     4381 atgcgctcga gcggcactgg atatagtcgg ccatattcgt caatgccaat tacctggggc
     4441 gaacattagg gctcacatcg gcatccatac ggggcttgtc atctcaacga accaagaggc
     4501 cgagcttgat ggcatatgcg ttgataccgc cgtgcagctg tcctttgcag cgcccgctaa
     4561 tagcattcaa tgttctcacg ccacctacca aatgctgtcc atgcatttag attttacaca
     4621 ccaaaaccag tgttattttt tatccggcga gcgccaagta gaagcatttg gctttttgcg
     4681 cagccatcaa agcagccacg aattggtggg gcgcaaggag gaattagcgc atgtgcaaca
     4741 ctttctggcg caacccaccc ctagctattt acatattcac ggagaggcgg gcattggaaa
     4801 atcacgcttg gtttttgaag tgcgcagcaa tgcacaggcc tatcaacact atattgcgca
     4861 atgtttgcct gagcaccagt attctggttt ataccctata ttgcgcttgg tgcaactgga
     4921 gagccagata ctaaacacaa cagcggcgca agccgtacaa cagttgcgcg caaggctgga
     4981 gcgcatgaac ctgccagcca atgcgctcac gcttttatgt atttggctga atttaccgct
     5041 cccgacggag caaccggcga gcatagaggc gctggaagaa caaaaacaaa ctttatttaa
     5101 aactctttat gcgctattac acggcagcgc caccgaacct acagccaaat tatttatcat
     5161 cgaagatctg cactgggcag atccattgac actggaattt ttagcctatt cacacagtca
     5221 atcaggctca cttgcttcgc ctcacaaatg gattggcact tcacgcacag catttccaat
     5281 cgaagctgca tgcagtcagc gccaaacact ggccatcaat aaattaaccc cccaccagat
     5341 tcaattgctg gtatctattt tattagagcg gcaaaccctt tcgctgcggt tgattgacct
     5401 tatcatcgag cgcaccgacg gtgtgccact ctatgttgaa gagctaaccc gcatgctcga
     5461 acgcaaaggg ttaatttacc gtttaaatgg cattgttgat ttaactagcc cgcaggcaat
     5521 tgaacaggta cccagcagct taaaagattc cctacagcaa aaattggatt atttaagcat
     5581 gagcaaggaa accgcccagc tcgctgccgt catcggccgc acttttgggt acaccctgtt
     5641 gctgcaagca agccctcacc cggaagaaaa agtacagcaa gacctaaaag agttaataag
     5701 ccaggattta attgttctgc agcgccacgt acacggggat cgctatattt ttaaacacgc
     5761 cttggtgcgt gatgccgcct atgaaagtat cccaagccca caacgtaaac tactccacca
     5821 caaagtggcg cagtgcctgg aggcgagcga ggaatctatc gacaatacta ttttggctca
     5881 gcattgggct aaggcggggg attttgttaa ggctagccac tacacaattg cccaagccaa
     5941 tgaagcttta aaacgctcat ccgctagtga agccatagta cttgcacagc aattagaaca
     6001 atgggcagcg aatattcccg acgctactcg cagcgagcat caactggcaa cctataggct
     6061 ctttacaacc gcctatatgg aatcaaaagg ttgggcctca gatgaagtta agcactacac
     6121 agaggcctct atcgcaattc ttaaacaaca aaataaagct gaagaaatag taccgcaact
     6181 ctggtggaag gtattgggtg gagtggttgc cggagcgcgt gctcccctag caccgacgat
     6241 agctgaaatg gatttaattt ttgatagtgt gaatgcaatt aataaatctg ccatacgctg
     6301 cgcgcaagga ttttattatt ttgcggaagg gcgtcgcgat aaatgtataa gctgttttaa
     6361 agactccatt gcctgctacg attccagtat agatatagct caccagcaaa catttggctt
     6421 tgatgtggcc gtgtttgcta aggctaccct aggtcgcacc tatgtagaca tgaacgaaca
     6481 ggcattagcg attcaaaacg tcacccaagc ggtacaagag gcgcgatccc acaacaacat
     6541 cccgtcacta ggcattgcat taatgtatta cgcaattgtc ttccagcaat atggcaacaa
     6601 accgcaagtc cagcaggctg ctggtgaatt gctggctttg gcggaaaaac atgacatggt
     6661 ggtgtatcac ctctatggcc agatgctcta cgactgggcc gtcggcacca ccgaaaatgc
     6721 agcggagata ttacagaagc taacggaaat ggggtctttt tttgccgtgg ggcaatttca
     6781 gtctttttat gccgacactc tgatagaaaa ccagtattac aaagaggcaa tttttttaat
     6841 tgagcgatgc ctgacaatca acaatgaaca caatgaacat ttttatacac cgcatttgct
     6901 ctcacgacaa ttatattgct tggcagccac ggatgcgggc agcaaacaaa tacaactcgt
     6961 agagactgct ttaacagaaa gactaaaaga aactccagta ccttttttaa acctccaata
     7021 accaacaagg gtcacattta tggcacatca gaacacactc atgaccgtca gaacaaacgt
     7081 tcttagagca atcgcttttg catgggaaaa taaagacgac ttagaaaatg gaaagtttta
     7141 tgggaaggtg gggcttgatt atatgctaag caaagccaaa gcgagcccga taggcttaac
     7201 cagcctagaa aatatggaat ccgtgaatat tcaattcatg aaacctaaag atactctcaa
     7261 gtataatcca ggctcggaag gttattcaaa agactggatc aaagaaccaa atcaggaaat
     7321 cattaccatt tgccttcctt catgccccac aaaacaaaat ggtgatacgt ttaccccgct
     7381 agagaaagct agcgcgctca tgaaatatta tgaactttcc cctcatttct ttcatgaatc
     7441 atcctttgcc aataacaatt caatttcggt ggttccaaag cttgccctca atgtgaatac
     7501 aggcttatca gctcttaatt ttttccccgg aacacatcat gctctcctag aaaaagagca
     7561 gattttacga ggcctgcagg caataaaatt cgcaaacatc gaacaatttg aaaaggatat
     7621 caactatgga aatgagatca taaatgcgtt acaagctgag gaaaacagag aaaaattggc
     7681 caccatccaa aaggaattgg ccagaaatag aaataaagat aagctgggcc tttcagaaga
     7741 tggttttctt tcattcgggt ctatagtact tcagatcatc gcagaatcct ggaaaaataa
     7801 agatttccaa aataaatatg actttgacat gcaccaaaat gagatcgaaa aatatgacaa
     7861 tgaagttaac actttgctta caaaggaatt ccaatttgaa tctctttggg atatgaagat
     7921 acgctttgtc tttacaaagg aaggcgaatt taaagatagt atagatcgga gttcatttgg
     7981 tctatcaaat gatagggccc gaatcaacgg ggtgcgcaca cttattgaag tggtattgcc
     8041 accttgccct gataatgatg atgatattct tcccgcccta gccgcctaca actgcatagg
     8101 cccgcgctac cccttcacct gctcctaaac caatccccta agcaaatttc cgagtaaacc
     8161 ctatgagcgc ccactgcacg ccttattgga atcccagttt tatctgtgaa ccccataacg
     8221 aagcctgggt tttaattagc gagggaaatc actacagcat caacgggcag gaagcccaaa
     8281 ttttagatga tctaatccaa aataatggac agctttacca ctatttattt gcacaacccg
     8341 atacccttac ccaagtcaaa cgcctgcgat tttgggatta tgttcgccaa ttaaaccttg
     8401 ttttagagtg ttcaccaact caaccgcaag ccactaattc ggcgggagcc attttgcccc
     8461 tgctcaatac ccagggcttt ttgccttatg ttgtactcga ccccattagc gacccagact
     8521 ggttaaccta cgccccgcag gccccctggg tattaataaa accctttggc gcacaaccga
     8581 gtatcggccc tatttttact ggcgagggcc catgccctca gtgtttacac acgcggttac
     8641 tcaacaatca gcctgcgcgc cgctggcagc aatttaaacg cggcttaacg ctaccgcctg
     8701 cagtgcccat gcaagaccaa acaccccacc tagccaaaat tgccgatttt tttaaccagt
     8761 atttagctgc cgccgctcgc aacccagctc acgcacactg catgtgggtg ttaaataagg
     8821 atttgaatgc ctacacagaa caccgggttg cgaccttacc tcagtgcccc cggtgcggtg
     8881 acccacacct aacacagcac acctttactg cccccatcaa tctaacaccg gtctttgcca
     8941 atgccgattt agatggcggc taccgcactc aaaatcgcga agatcttttg caggaattaa
     9001 aacactgcat tagcccggtc accggcgtac tcacggaatt aacacatatc agtgcagaaa
     9061 aagcagagca gttaatgatt tatcaggccg cctattccac ccaagtagta gaagagaatt
     9121 ttgtgcaatt atctttaggc aaaggtgttg ctaacactca agcacagtta agtgcgctgg
     9181 gggaagcctt tgagcgcaaa gcggcccagt accaaggtga tgagcccttg cgttacgcat
     9241 taccccaaga gttaccaggt aaagcctatc taccgcagga attggcagcg tttagctcgg
     9301 cgcaatatca gttttttgcg caatgggagc aggcaaacct gcagcaaccg caatgggtaa
     9361 aaccctacgc cgccgataca cctatttatt ggtgcccggc gtggaattta actcggcaca
     9421 ggccagtgta ttttcctgct gcattttgtt tggccgatac cccctttgac gatgcccact
     9481 attctatcta cacccacaat ggcaattcgg cgggcgccag tattgaagag gctattctgc
     9541 aaggagcctt agagttaatt gagcgcgatg cagcggccat ctggtggtac aaccgtattc
     9601 cgcgcccgca aattgcttta gaggtaatag caccggccca gcaaaaaata attgccaata
     9661 ccttaggcaa cgagtgggat tattggttat cggatatttc ccacgatttg ccagcggtta
     9721 gttgcgtagc cgtcgggcag cataaaatag acggccgcta tgtgttaggc ttcgggtgtc
     9781 atttggaagt tagtatcgct gcacaacggg cacttaccga gctgtatcaa ttaatttgca
     9841 ttaaggataa ggtaagtggc ccctttgatt ttaaagccat taagccccac ccatttttgt
     9901 acccgcgcgc caatacagct attaaatatt caggcgatta ttgcgcccgc caaaatgtaa
     9961 gtttacacag ccatatcatt gatctgatag aagcgttaca agcgaatcag cttgacttat
    10021 gtgtggtgaa ttattctcgc gcggatgttc cgttaaaaac gctcaaggtt gtggcgccag
    10081 gtttatgcca cttttggcct caattggcca cgcctaggtt atatcaggtg gctgttaagc
    10141 tcggctggtt aagccacccg ctaaccgaag cggaattaaa tcctcaagcc ctttatttat
    10201 aaggtacacc aatgatttta tccgccgccc cagccacgtc tcgccactcg attaaacaca
    10261 ttttactgtg cggcagtctc tacggccgtg tgtatttaga cgcgttatac aaaacgcgtc
    10321 atttacgctt ggccgctata ctggcgcggg gaagccgtac atcgactcta ctggccgaac
    10381 aagcaggtgt ggcatcaatt acctatttag atcaactagc caccacaccc gatgcagcca
    10441 tagtggccgt tggaagtgaa gccggttttc atttggcgca ggcctgttta aaaagacgta
    10501 ttcctgtgtt gcttgaacac cccgtattac ccagccattg cagcgatttg ttagccttgg
    10561 ctcaggcaat ggacgtgcgc ctgcatataa acagccattt tagtttatta cctccggccc
    10621 aagagtttat tcaattatgt cgagcgctca actctcaggc gcaacctctc agtgtacaaa
    10681 tcagctgcaa tagccgcacc ttattttcct tgctcgatat tgcgcaagcc atttttggct
    10741 tatttaattt tgaagcactc tgtagcgatg ctctagatgc cgaagaacat tattatcaga
    10801 tgcgttttaa actcaataaa ataccctgca ttgtgcacta tcaaaattgg cgcagcgccc
    10861 aggatgatag tttagattca cccctcggcc atcaatgcac tattacctac ccgcaaggta
    10921 ttatgcaatt aaatagcagc tttggcccat gcctgtggtt tccactgatg gccgccacta
    10981 caggggtggc ctatcccgcc tattccagtc taagccgcct ggatcaagcg cccactcacg
    11041 cggatataat cgcctggcgg gaagatgcga atattcaggc aattgaatca ttaattaccc
    11101 ccgatgaaga ccaatttaca cagacacccg tctatttaac tcacctgtgc caaaactggc
    11161 atacactgtt cactgcacat ggatttaaag tagcgcctcc acaggtcaac aaactaacag
    11221 atatattttt tacagccaaa caattagtgt gcagtgcgaa gcggtaaaat tcatggctac
    11281 gctcatcaac tcaattaccg gtgcaaccat taaactgcta ccggtgcatc tgttcggccg
    11341 gttggcggaa acctgccata ctgtttttga caatcattat atttcccgtg tacacgccac
    11401 cattagctgg accgggtgta cctggatact cagagacagc agtattaacg gcacctacat
    11461 tgatcgtgtc ttggctgcag ggaccactca gcatgtttta gcactcaaca gccaactaca
    11521 atttggcaat ttagatgccg acccatggca agttgcctgt ttagcgccgc cagtgaccat
    11581 gctcgtcccc ctgcatccga atttacccta tattgaacta gacaaaataa ttgccctgcc
    11641 cagcgagcaa gaacctgcgg tagtaatttc ggaaattgcg cccggtgatt ggttgtgtga
    11701 aagtgaaatg ggccaagcat tacttaaatc aggtgattta gttggctctg ccaaccacat
    11761 ttggcgcttg gtaatagcca atccattcca agatacaata gtcgatcctt ggcctatcga
    11821 ggatgcgtgc acagaagtgc gattgcactt tcacgccagt caaaatgaag agcacgtgca
    11881 attaaaaata cacattggtc aaactattat tgatttacaa gagcgcacac accactatgt
    11941 gttgctgtta ttagcccggc aatatacaca ggacaccgaa gcacagcgca ttgaaaccga
    12001 acgcggatgg attgataaag agcagctatg ccgacagatg ggcgttgatt taaaccacct
    12061 caatatatta atttttagat tccgcaagca gttaatgaat gcgctgccac cccacgtgca
    12121 accgccgtca attgtacaaa cgcgtgttgg tcaggtacgt atccagtgtg ctgaaatttc
    12181 aattacgggc gggcttccga cacacaccca acatcaaagc agctagtcag taagccgctc
    12241 gctcttcagg caataaatct gcctcataac aagaattgag aatctctcag tgattggttg
    12301 caaggccatg taatcatgtg ccgatccacg gcacaaaacg gcagattttt agtcccaaat
    12361 atctttaatc agccacgtct gaagcagtct tctaaatgtg ccgacatttg taataccgtg
    12421 taatcgacag gcatagatag cggattaaca atggcgcatc caattcataa ctatggggtg
    12481 cgtcatgaaa cataaagtac aaaatttact gttgttcttt gctttaagcc aggctcaaac
    12541 atgggccgat ggcacaccac cttcgcagcc aaaatgcata aaaccagagc gtccggcgtt
    12601 gccgtcactc aatgataaag acctgaacga ctccataaaa cttgctaact gggtaaaaac
    12661 ggcatccggc cagtacaatc aatacttggt aaccacaaaa gaagggaaat ccattagctg
    12721 cgccaacctc gccgcagaat tatcaaacac cactcttgaa ttgggctcag aaattaacag
    12781 cacaaagaat aagaagctga ccgcgctgaa cacatttgct gacacctgct cacaggaact
    12841 tgcgtattta ttggcgcaag aaaaagcatc cctagggcca gatgattgca aaattgaggt
    12901 tgttaatagt gatatcgaca aactaaattc ggcgcaagta caactaaaag cagagcaagg
    12961 cttgctatct caaagtgtag accccgacca acccgtcaaa aatttttcta aggagcaatt
    13021 acaatctctg ctcaacggcg ataaattcgc cgacatagcc acagaaaagc cttattatgg
    13081 cgtgttagaa ataggtgcaa gtttaatgcc caagtataac aaagaaggca acaatgaagg
    13141 gtttcaagat gctaattatt acgcagcact taagctcaac aatcgttggg cgctaaaaga
    13201 tacaaaaact ccttatctaa attggttcaa accctcatgg gttgcctacc aggaaatgaa
    13261 tgtgcttttc tattcggcac ccatcgcctg cgaaaagaat tccgagcccg ccactaacga
    13321 ctcgccggac gatccggctt taaagtccat cgaagaatca cagggttcat gtaataaagc
    13381 cactgatatt ggcgctctaa aattcaagga tgtttcccac accgtaaatg cctcagccaa
    13441 cttctcattt ttgtatgcca ataaagacaa tatcggcgat tgggaaatcg gctttaacgc
    13501 tcgggtaggg ctattaaatc gggagaaact cagtccagat cttgattcgg tttccaccta
    13561 tagttcaggt ggggttgaat tcaggctgaa tgacttttta ggtaaaaata tagctggaaa
    13621 aacttatcgc aacggtttgc ctcgttttgt ttt
//
LOCUS       DAOQGS010000033        13496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2123_length_13496_cov_4.628376, whole genome shotgun sequence.
ACCESSION   DAOQGS010000033 DAOQGS010000000
VERSION     DAOQGS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13496
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_13496_cov_4.628376"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKZ52_02220"
     CDS             complement(<1..744)
                     /locus_tag="PKZ52_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58611.1"
                     /translation="MENTLTHLAFLAGAGLSLALTITLWFTRKSRALHPSIALATAVQ
                     SVWLFSLAYWYAPGSWPSQVWVSSLECLHYSTWLLTLARIIPFYCERPLPTLYRFFIL
                     GLSLVFVFANGINAFWPEASPFNTQRLMVWQGLVLSIGCLVTIEQLYRNITSVRLLKL
                     VALNLAAMFIYDIYLYAQDLIFDNASNFFAQVRATVTIAACTFMMLATLVLKHHNAQP
                     PQLVLSRPIAFYSTSLIIAGTLLSAISFAG"
     gene            complement(875..3139)
                     /locus_tag="PKZ52_02225"
     CDS             complement(875..3139)
                     /locus_tag="PKZ52_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HNG58612.1"
                     /translation="MFKHTSKPGSFDVITPKGREPMQPSKLLGQIRTQTLLGCEQLLA
                     HMFSAVDDLFYDLSKRASSNNEANLYFEAMREIRVNREGTAKRFLHQVGSAFAHLADR
                     ERFGSAPAKEHSNINLSLVEGDDLEIDLAKTTMISSARALFNDDLYHLTARLNELLPL
                     LDIDENSNPLDPEQLTKAFIIACQEQLHINIKARLIVFKLFEKHLLKQLGHIYHDANQ
                     LLIEAGLLPELARSVKKHAPQMPATSLDELTGQGSHTASAMARTPPQAAPFFMGQEAL
                     GLLLSAVRQSTASSPAGTAMPAINCFIFTSNPGPVMSGQELATRLSQSQPVVEEKIAQ
                     APRNVIGDIVSDLLASNDPELPHALDQDDENVINLVAMFFDQILADDNLPSVVQSLIC
                     RLQIPILRMAIKDSSFFDNPEHPARLLVNSLCSAGLELDDSRPLDKDPAYKKMLDVVQ
                     QICAHQQQDESVFAEARAQLVDLLEKEKRKSGLVEQRTVQAEEGQYRMLQARLVAQNL
                     IYEKIKDQALPDEISEFLCGQWLQVLVFLHLRFGEDSAQWMGAEQSIIDLVWLSHDHK
                     DQRSQQRRERMLPELLDRLDQGLEGVITDAVERNQLVDKLEAILKAVGAKQLDGRQFH
                     SLTSLQKEELGRGESAQKSWQEMSVIERQMARHEELFNQYYVEAKEMPIGTWIIYADE
                     TGRLQRCKLSSKIDSDTYLFVNRLGFKVLEKSRKRLALDLQNGRARVINTQPFFDRMM
                     TLIVNRLENAQGDT"
     gene            3484..4059
                     /locus_tag="PKZ52_02230"
     CDS             3484..4059
                     /locus_tag="PKZ52_02230"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNG58613.1"
                     /translation="MVLMLDNYDSFTYNLVHYIQELGFGVRVVANDEWSLEQVGDFAP
                     SHIVISPGPCTPNEAGISLQVIARFGGLVPILGVCLGHQAIGQALGGQVVRAPRAVHG
                     KTSLVHHDGKGVFAGVPNPFSAVRYHSLVLAEDNLPAELEVSAWTESPNEPAVIMGVR
                     HKFWPLEGVQFHPESILSEHGHQLLQNFLRL"
     gene            4099..5145
                     /gene="trpD"
                     /locus_tag="PKZ52_02235"
     CDS             4099..5145
                     /gene="trpD"
                     /locus_tag="PKZ52_02235"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNG58614.1"
                     /translation="MQIKEALSRLVEGGSLKQAQMAAVMRQIMTGEATAAQIGGFLVA
                     LKIKGETIDEIAGAVQVMRELATPVAIAPGRVPLVVTCGTGGDGSPLFNVSTAAAFVV
                     AAAGGRVAKHGNRSITSVSGSADVLEAAGVHLGLTPEQVARCVEQLGVGFMFAQAHHS
                     AMKHAIGPRKELSLRTIFNILGPMTNPAGVKRQVLGVYSRSLCNPLAQVLGRLGSEHV
                     LVVNADDGLDEISLAAPTHIAQWKDGVVSEFTIAPEDFGIERQSLDGLKVSSAAESLS
                     LIKDALGKRATERGRAAADMVALNAGAAIYVSGLADSLAQGIVMAEDTIATGLALEKL
                     TDLAEMSQVLASAG"
     gene            5220..6020
                     /gene="trpC"
                     /locus_tag="PKZ52_02240"
     CDS             5220..6020
                     /gene="trpC"
                     /locus_tag="PKZ52_02240"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNG58615.1"
                     /translation="MSHQPPTILRRIIDRKWAEIQERSQKRSLADLERIAGQAPKGRG
                     FEQALIERIERKEPAVIAEIKKASPSKGVIRERFDPVEIARSYEAAGAACLSVLTDVD
                     FFQGADAYLQAAKEATGLPAIRKDFMVAPYQIAEAKVLGADAILLIVAALAPSELIAL
                     NTYARSLNLDVLVEVHNRAELELALDLPNALVGINNRDLHSFNVSLNTTYDLLPMIPQ
                     GKYVITESGIFTRDDVAAMNAQGVFGFLVGESFMRAPVPGEKLRELFF"
     gene            complement(6099..6734)
                     /gene="crp"
                     /locus_tag="PKZ52_02245"
     CDS             complement(6099..6734)
                     /gene="crp"
                     /locus_tag="PKZ52_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HNG58616.1"
                     /translation="MVSVSLSPHIRNVETFLAHCHRRRYPAKSTLIYAGDKSDSLYYI
                     IKGSVTVLIEDEEGREMIVAYLNDGDFFGEMGLFDEKDARSAWVRAKSECEIAEISYA
                     KFQEISETHPEFLFALGSQMARRLRATTRKVGDLAFLDVTGRVARTLLDLCKEPDAMT
                     HPDGMQIKITRQEIGRIVGCSREMVGRVLKTLEDQGLVAVKGKTMVVFGTR"
     gene            7106..7528
                     /locus_tag="PKZ52_02250"
     CDS             7106..7528
                     /locus_tag="PKZ52_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNG58617.1"
                     /translation="MQATVKWVDGTLMVGESGSGHAVVMDGPPDHGGRNMGLRPMEMI
                     LLGVGGCASFDVISILKKSRQQIVDCHCELSAERAEGVPSPFTKIHMKFIVTGRELKP
                     AQVEKAVQLSAEKYCSASIMLRAAGVAISHEYEVVEAI"
     gene            complement(7545..7997)
                     /locus_tag="PKZ52_02255"
     CDS             complement(7545..7997)
                     /locus_tag="PKZ52_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG58618.1"
                     /translation="MQVILPIQQQEFNLPVSLAAQPDLLDIANYYQTGRGNFWVALAN
                     DRVIGTIGLRDLGNGQGALRKMFVACEFRGAPLGVANLLLNTLIEWAKARKISDVYLG
                     TTEYFHAAHRFYEKNLFIAIARESLPPSFPLMAVDTIFYQRSLLSCAR"
     gene            complement(8059..8688)
                     /gene="coq7"
                     /locus_tag="PKZ52_02260"
     CDS             complement(8059..8688)
                     /gene="coq7"
                     /locus_tag="PKZ52_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNG58619.1"
                     /translation="MNLRDQLISQFDSALRSLTGGTEPLLRPSPAAGMAETELNAQEK
                     RHAAGLMRVNHSGEVCAQGLYQGQALTARLGDTRDAMQRAAQEEIDHLAWCEARLNDL
                     NARPSLLNPLWYGLSFGLGAAAGLVGDKISLGFVAATEEGVCQHLNEHLKSLPPEDHK
                     SRAIVNQMLVDEHHHEQTALAAGGVSLPAPVKALMRLTAKAMTRSSYRI"
     gene            complement(8937..10883)
                     /locus_tag="PKZ52_02265"
     CDS             complement(8937..10883)
                     /locus_tag="PKZ52_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58620.1"
                     /translation="MYSHSKLAQRSWPKFSLLACYSLLSASFFCANLTHALTPSNSSS
                     SSSSSSSGGAEDSSSGGVCEPQDLFIHDSSSQSVAGAIAEGDCSQPNDPQSLFDRFTF
                     TLQQGQHIAFSYDIGVTPVHIKVLDSQGVTRGESSGGYASIPAKGTMLALEAGTYSLE
                     VAFVDPAVSGQYFINVVTSNPAPALIDQETLAAKCSSIWRLAPINERLFLPITCSSGS
                     LGTDRFYIHFSLDLPEESDIALDLASADFAPYLTLTNEQGEKLLQAQSEVNKPLRVPA
                     ENYLKLPAGKYALELTTALPYTWGAFNFTFTNLNWQGTTSSSSSSGLTSSSSSSSSSS
                     SSTSTSTSSSSGWWSNSSSSTSSSGWGNSSSSSSGLASSSGGWASSSTSSSGFGGSSS
                     STSSSSSSGRSSTSSSTSTSSSSSGAAGCNQLLKLNSVVKGELHSGPCLMGPEENLRS
                     FQNYIVHLTPGQPVVFALGATFDSVLSLKDARGRTLLSNNGDGNGARIPASGSYSVQK
                     AGDYLVQVTTKEVRTLGSFQLEAKTAADPCAAVKPLIAGAFMSGSLEQGDCRTTKGGQ
                     FIDRYSFKGQNKTQVMLELDAKFPSKLTLRGPDGKVLASDSTTIPHRGFLKLPKGSSG
                     EYTVEVSSSKPATGGYVLQFHQKN"
     gene            11504..12112
                     /locus_tag="PKZ52_02270"
     CDS             11504..12112
                     /locus_tag="PKZ52_02270"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNG58621.1"
                     /translation="MSKLIMGLDGKQRCSWCSSVTEFIPYHDKEWGFPVGDDVRLFEK
                     LCLESFQSGLSWRTILAKRENFRAAFAGFNFNKVARFTQKDITRLLQDEGIVRHRGKI
                     DAVINNAQCAVDMVKKEGSLAAYFWRFAPKTHPAEIAQTQSTSVESIALSKELKKLGW
                     KFVGPTTVFAFMQAMGLINDHVEGCWVRSEVEKARKKFVQPK"
     gene            complement(12603..>13496)
                     /locus_tag="PKZ52_02275"
     CDS             complement(12603..>13496)
                     /locus_tag="PKZ52_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58622.1"
                     /translation="PTIALGCLVALIPYGYFAFENSAGLTTRFKELSFIDDDISTLAK
                     LSHFVHNYGKYFSFEFLLLKGDENLRHAIGYAGEIFFTTFILAIIGIFTFLKTHDRQR
                     IFLRLLVINLAFAPFAAALTNGSAHALRGMLGPVYMAYLACYGFNFLLQHTSKFSLRI
                     SKGLLWILSIEVACFLFAYFVVYPGRSLKATEDYDSLSRLQEAVHRAPDKIYFTGASY
                     ANLRFFLEFIDNPKSIPFEIKGDEVTPKPNECVVYHDYNKIEERLGTPVYEKKSVYSA
                     NWLQKMIGIEDVPHGVRLRCY"
BASE COUNT         3096 a         3260 c         3820 g         3320 t
ORIGIN      
        1 acccgcaaag ctaatcgccg acaacagggt gccggcgatg attaaagagg tggaataaaa
       61 cgcgatgggc cgcgacagca ccagctgggg cggttgtgcg ttgtggtgtt tgagcaccaa
      121 ggtggcgagc atcataaagg tacaagcggc aatggtcacg gtggcgcgca cttgggcgaa
      181 gaaattgctc gcgttatcga atattaaatc ctgcgcgtag aggtaaatgt cgtagataaa
      241 catggcggca agattgaggg cgacgagctt gagcagccgt acgctagtga tattgcggta
      301 gagttgttcg atggtgacca ggcagccgat ggataacacc aggccctgcc acaccatgag
      361 cctttgggtg ttaaacgggc tcgcttccgg ccaaaaggcg ttgatgccgt tggcgaacac
      421 aaacactagg ctcagcccca gaataaaaaa tcggtaaagg gtgggcagag ggcgttcgca
      481 gtagaagggg ataatgcggg ctagggtgag tagccaggtg ctgtagtgca ggcattctaa
      541 gctgctcacc caaacctggc tgggccaact gccgggggca taccagtagg ccaggctgaa
      601 cagccagaca ctctggacag ccgtggccag tgcaatgctt gggtgcaagg cgcgggactt
      661 gcgggtaaac cacagggtga tggtgagggc cagagagagg cccgcgccag ctaaaaacgc
      721 caaatgggtt agggtgtttt ccatgtgtgt gcagccagtc agtgcggtgt ttggctcggc
      781 gaagggtgcc aatgtctggc ttgttgactc gtttatccgg caagtccacc gctgttttaa
      841 tattgtgtac cgcgcaagat taaccggtga gagcttaggt atcgccttga gcgttttcca
      901 ggcgattcac aatcaacgtc atcatgcggt caaagaacgg ctgggtattg ataacccgcg
      961 ccctgccgtt ttgcaaatcg agagccaaac gcttgcggct tttttccaac actttaaaac
     1021 ccagccggtt cacaaacagg taggtgtcgc tgtcgatttt ggatgagagt ttgcagcgct
     1081 gcaggcgacc ggtttcatcg gcgtagataa tccaggtgcc gatgggcatt tccttagcct
     1141 ccacataata ctggttgaac agttcctcgt gacgggccat ttgccgctcg atcacgctca
     1201 tctcctgcca ggatttttgc gcgctctcgc cgcggcccag ttcttctttt tgtagggatg
     1261 tgaggctgtg gaattggcgg ccatccagct gtttggcgcc cacggctttg aggatggctt
     1321 caagcttgtc caccagctgg ttgcgttcta ccgcatcggt gattacgccc tcaaggcctt
     1381 gatctaaacg atccagcagc tctggcagca tacgctcccg gcgctgttgg gagcgctggt
     1441 ctttatggtc gtggctgagc cagaccaggt cgataatact ctgttcagcc cccatccact
     1501 gggcgctgtc ctcgccaaag cgcaggtgta aaaacactag cacctgcagc cattggccgc
     1561 agagaaattc gctgatttca tcgggcaggg cctggtcttt gattttttcg tagatcaggt
     1621 tttgcgccac caggcgagcc tgcaacatgc ggtactggcc ttcctctgcc tgcacggtgc
     1681 gctgttcaac caggccggat ttgcgttttt ctttctccag caggtcaacc agctgggcgc
     1741 gggcttcggc aaagacagat tcatcctgct gctgatgggc gcagatctgc tgcaccacat
     1801 cgagcatttt tttgtaggcc gggtctttgt ccagtgggcg gctatcgtcc agctcaaggc
     1861 ctgcgctgca caggctgttc accaataagc gggcggggtg ctctgggtta tcaaagaagc
     1921 tgctgtcttt gatcgccatg cgcaaaatgg ggatctgcag gcggcagatg agggattgca
     1981 ccacgctcgg caggttgtca tcggccagga tttgatcgaa aaacatggcc accaggttga
     2041 tgacgttttc atcgtcctga tccagcgcgt ggggcagctc gggatcgttg ctggccaata
     2101 gatcgctgac gatatcgccg atgacattgc gcggcgcctg ggcgattttt tcctccacca
     2161 ccggttggct ttggctgagg cgcgtggcca gctcctggcc gctcatcacc gggcctgggt
     2221 tgctggtgaa gataaaacag ttaatcgctg gcatggctgt gcccgctggg ctgctggccg
     2281 tgctctgtcg caccgctgag agcaacaggc ccaacgcctc ttggcccata aaaaagggcg
     2341 cggcctgtgg cggcgtgcgg gccatggcgg aggcggtatg ggagccttgg ccggtaagtt
     2401 catccagtga agtggcgggc atttgcggcg cgtgtttttt tactgagcgg gccagttcag
     2461 gcagcaaacc cgcttcaatc aatagttgat tggcgtcgtg gtagatgtgt ccgagctgct
     2521 taagtaggtg tttttcaaac agcttaaaaa caattaagcg cgctttgata ttgatgtgca
     2581 gctgttcttg gcaggcgatg ataaacgcct tggtcagttg ttctgggtct agcgggttgc
     2641 tgttttcgtc tatatccagc aggggcagca gctcattaag ccttgcggtt aggtggtaga
     2701 ggtcgtcgtt aaaaagcgcg cgggcgctgc tgatcatggt ggttttggcc aggtcgattt
     2761 ctaaatcatc gccctccacc aaggagagat taatattgct gtgttctttg gcgggagcag
     2821 agccaaagcg ctcgcgatcg gccaaatggg caaaagcgct gcccacctga tgtaaaaacc
     2881 gtttggcggt gccttcgcgg ttgacgcgaa tttcccgcat agcttcgaaa tagagatttg
     2941 cctcgttgtt gctggaggcg cgcttggaaa ggtcgtaaaa aagatcgtca accgctgaaa
     3001 acatgtgggc gagcagttgc tcgcatccca atagggtttg cgtgcggatt tgccccaata
     3061 atttggacgg ctgcatgggc tctcggcctt tgggggtaat cacatcaaac gatccgggct
     3121 ttgaggtgtg cttgaacatg ggtagtatct gctaagcgac aataagtctg ggctaacggg
     3181 tgctttgagc aaattctatt ccttttttcc ggcatctcct accgcgctct cagattaaat
     3241 cggcgtagcc tgctaggtct ttgcattaca aaggaatttt acactgcact gaatcgggtg
     3301 ccgccatctg cagtactact ggcgcacagc tcacctctgg ggctgtaaac accatcgacg
     3361 agacctgcgt cacatttatt gtttttataa acaccgcgcc cgcctctggc ttggccgtgc
     3421 gggcgtgtac actttgcact tgcgccttgg ggtaaggctt taggttttta acgggagcct
     3481 gctttggtac tcatgctcga taactacgat tcatttacct acaacctggt gcactacatt
     3541 caggagcttg gctttggggt tagggtggtg gctaatgatg agtggtcgct tgagcaggtt
     3601 ggggactttg cacccagcca tatcgtgatc tcgccggggc cttgcacacc caatgaagcg
     3661 ggcatttctc tgcaggtgat tgcaaggttt ggcggtttgg tgcctatttt gggggtttgc
     3721 ctggggcatc aggccatagg ccaggctctc ggcgggcagg tggtgcgggc acccagggcg
     3781 gtgcacggca aaaccagcct tgttcaccac gatggtaagg gagtttttgc gggcgtgccc
     3841 aatcccttta gcgccgtgcg ctaccactct ctagtcttgg ctgaggataa tttacccgca
     3901 gaactcgaag tgagcgcttg gacagaaagt ccaaatgaac ctgcggttat catgggtgtg
     3961 cgacataaat tctggccttt agagggcgtg cagtttcacc ctgaatccat cctcagcgag
     4021 cacggccatc agctgctgca gaattttttg cgcctatagg cgcgcaggtt tttagcttta
     4081 acaatagggc agtatttcat gcagattaaa gaagcattat cgcgcctggt ggaaggcggc
     4141 tcgcttaagc aagcgcagat ggccgctgtc atgcgccaaa ttatgaccgg cgaggcgacg
     4201 gccgcccaaa ttggcggttt tttggtagcg ctgaaaatca aaggcgaaac cattgatgaa
     4261 attgccggtg ctgtgcaggt gatgcgggag ctggccaccc cagttgccat agcgccgggc
     4321 cgggtgccat tggtggtcac ctgcggtacc ggcggagacg gttcgcccct gtttaatgtc
     4381 tccaccgctg cagcctttgt ggtggccgca gccggtgggc gagtggcgaa acacggcaat
     4441 cgctctatca cgagcgtatc gggcagtgcc gatgtgttgg aggcggccgg tgtgcatttg
     4501 ggcctaaccc ctgagcaagt ggcccgctgt gtggagcagt tgggggtggg ttttatgttt
     4561 gcccaggccc atcactcagc catgaaacac gctatcggcc cccgcaagga actcagcctg
     4621 cgcaccattt ttaacatcct cggccccatg accaatccgg cgggggttaa gcgccaagtt
     4681 ctgggcgtat acagccgcag cctgtgcaac cctttggccc aggtgttggg gcgtttgggc
     4741 agtgagcatg tgttggtggt gaatgccgat gatggtttgg atgaaattag cctggccgcc
     4801 cccactcata ttgcccagtg gaaggatggg gttgtcagtg aatttacgat tgcgccggag
     4861 gattttggta tcgagcggca gagcctggat gggcttaagg tgagttctgc agccgagtca
     4921 ttaagcctta tcaaagatgc cttgggcaag cgcgccaccg agcggggcag ggcggccgcc
     4981 gatatggtcg cccttaacgc cggggcggct atttacgtct ccggccttgc cgacagcctc
     5041 gcccagggaa tcgtgatggc ggaggacacc atcgccacgg gcctggcatt ggagaaatta
     5101 actgatttgg ccgagatgtc ccaagtgttg gccagcgccg gttaagcggc ccgtgcagcc
     5161 ctaagcgggc cgtgccacaa tgggcggccc ttttaatcat ccagttgtag agaaataccg
     5221 tgagccatca gccgcctacc attcttcgcc gcatcatcga ccgcaaatgg gctgaaattc
     5281 aagagcgcag ccagaagcgc agcctggctg accttgagcg tatcgcaggg caggcaccga
     5341 aggggcgcgg ttttgagcag gctctgattg agcgcatcga gcgcaaagag cccgccgtga
     5401 ttgctgaaat caaaaaagcc tcccccagta aaggcgttat ccgcgaacgc ttcgacccgg
     5461 tggaaattgc ccgctcctac gaagccgcag gcgccgcctg tttgtcggtg ttaaccgatg
     5521 tggatttttt tcagggggca gacgcctatt tgcaggcggc taaagaggcc accgggctgc
     5581 ccgccattcg caaggatttt atggtggcgc cttatcaaat tgctgaggct aaggtgcttg
     5641 gcgccgatgc tattttgctg attgtggctg ccttggcgcc cagcgaacta attgcgctga
     5701 atacctatgc ccgcagcttg aacctggatg tattagtgga agtacacaac cgcgctgagc
     5761 tcgagcttgc gctggatttg ccgaatgcgc tggtgggtat aaataaccgg gatttacaca
     5821 gttttaacgt gtccctcaac accacctatg acctgttgcc catgatcccg caaggcaagt
     5881 atgtaattac cgagagcggc atttttaccc gcgacgatgt tgcggctatg aatgcgcagg
     5941 gcgtatttgg atttttggtg ggcgagtctt ttatgcgcgc ccctgtgccg ggggagaaat
     6001 tgcgcgagct gtttttttaa cttaaaaaat aacatcggaa actaaaaagg ccgcccaagc
     6061 acacttgggc ggccttttta gttgtcgcag cgattggctt aacgggtgcc aaacaccacc
     6121 atggttttgc ccttcaccgc taccaaccct tggtcttcca gggttttaag tacccggccc
     6181 accatctcgc gagagcagcc cacaatgcgg cctatctcct ggcgggtgat tttaatttgc
     6241 atgccgtccg ggtgggtcat ggcgtcgggc tctttgcaca ggtcaagcag tgtgcgcgca
     6301 acccggccag tcacatccaa aaacgccaaa tcccccacct tgcgggtggt ggcgcgcagg
     6361 cggcgggcca tttgcgaacc caaggcgaag aggaattccg gatgggtttc ggaaatctct
     6421 tggaatttgg cataactaat ttcggcaatt tcgcactcgc tcttggcgcg aacccaagcg
     6481 ctgcgtgcgt ctttctcgtc gaacaggccc atttcaccga agaaatcccc atcgttgaga
     6541 taggccacaa tcatttcccg gccctcttcg tcctcaatta aaacggtgac tgagcctttg
     6601 atgatgtaat acagggaatc gcttttatcg ccagcgtaaa tcaaggtgct tttggcggga
     6661 tagcggcggc ggtggcagtg agctaaaaat gtttctacgt tacgaatatg cggggagagg
     6721 gagactgaaa ccaagatgcg atcctcgtga ctagaagaat tcgattgtta ttgttgtgta
     6781 ggcctgtgct aagtaatcag cataaggccg tcttctatcc ttataccgcc aaaactctaa
     6841 cctgcgcaac aattaaccga gcctattcat tagtgaattg cttacattat agacgctgtt
     6901 aaaaaatccg gcggtatacc ggacagatta tgcgctcggc gtgccaggct caccagcatt
     6961 cccagtacat aatgttagat agtccgcata aaaaatgcca aattaaccga acacattggc
     7021 agccctacaa attgatgggg cttcgtgacg cagacctaag gccctgataa tctagcttct
     7081 tttctttatt tgggcgggtt gggttatgca agcgacagtt aaatgggtag atggcaccct
     7141 gatggtgggt gagtcgggtt caggacacgc ggtggtgatg gacggcccgc cggaccatgg
     7201 cggtcgcaat atgggtttgc ggcccatgga aatgattttg ttgggggtgg gcggctgcgc
     7261 ctcctttgac gtaatcagca tcctgaaaaa atcccgccaa cagattgtgg actgccactg
     7321 tgagctcagt gctgaacgcg ccgaaggtgt gccttcgccg tttaccaaaa ttcacatgaa
     7381 atttattgtc accgggcggg agttaaaacc tgcgcaggtg gaaaaagccg tgcaattatc
     7441 ggcggaaaaa tattgctcag cttccattat gctgcgcgcc gcaggcgttg ctatcagcca
     7501 tgaatatgaa gtggtggagg caatctaata tttgcagcgt ttttttatct ggcgcagctg
     7561 agcaggctgc gctgataaaa aattgtgtct accgccatca gcggaaaact tggcggtaaa
     7621 gactcacgtg caatagcaat gaagaggttc ttctcgtaaa atctgtgggc tgcgtgaaag
     7681 tattcagtgg tgcctaaata cacatcgctg atttttctcg cttttgccca ttctataaga
     7741 gtgtttaata ataaatttgc cacacccagc ggcgcgcctc gaaattcgca ggcgacgaac
     7801 attttgcgca gggcaccctg tccattgccc aaatccctca agccgatggt gccaatcacg
     7861 cgatcattcg ccagcgccac ccaaaaattt ccccgcccgg tttggtaata attggctata
     7921 tccagcaaat ctggctgagc agccaaactg accggcaggt taaattcctg ctgctgaatg
     7981 ggcaaaatta cctgcactat tcctgctgta tgcgcagggg tgcatgaaat tacgcttatc
     8041 accgcgctat ttacgcaatt aaattctata gcttgagcgg gtcatggcct tggcggttaa
     8101 acgcatcagc gcttttactg gcgcaggcaa gctgacgccg cctgcggcca gggcggtttg
     8161 ctcatggtga tgttcgtcca ccaacatttg gttcactatg gcgcgggatt tatgatcctc
     8221 agggggcagg gattttaaat gctcgtttaa atgctggcag acgccttctt cggtggccgc
     8281 cacaaacccc aaacttatct tatcgcccac caatccggca gctgccccca atccgaatga
     8341 caggccatac caaagcgggt tgagcaggct ggggcgggcg ttaaggtcat ttaagcgcgc
     8401 ttcacaccag gccaggtggt caatttcctc ctgggctgcc ctctgcatag catcacgggt
     8461 atccccaagg cgcgcggtga gggcttggcc ctggtagagg ccctgggcgc acacctcgcc
     8521 gctgtggttt acccgcataa ggcctgcggc gtggcgtttt tcttgtgcat ttaattccgt
     8581 ctcagccatg cctgcggcag ggcttgggcg caataggggc tctgtaccgc cggttaagct
     8641 gcgcagagcg ctgtcaaatt ggctgatgag ttgatcgcga aggttcataa aacgcccctg
     8701 aaaaattatt cggcattgta cctaagtgca caggctgaat gagtgtgcag cacgccaagc
     8761 gactatcaga acaggctcag tctacaggca agtgacttta cgctctgagg gtatgggcag
     8821 acctaagtag gcgccctcgc taaaaatatt gcagtgcttt gctccaaaca caaaaaccca
     8881 ggcaattaaa aattgcctgg gttttgctcg atccttcttg gcgttaaaaa ggcgatttag
     8941 ttcttttggt gaaattgcag aacatagccg ccggttgccg gtttgctgga ggaaacttca
     9001 accgtatatt cgccactgga gcccttaggc agttttaaaa accccctatg gggaattgtt
     9061 gtggagtcgc ttgccagcac ttttccgtcc ggcccgcgca gtgtgagctt cgagggaaat
     9121 ttagcgtcta gctccagcat cacttgggtt ttgttttggc ctttaaaact atagcggtcg
     9181 ataaattggc cgccttttgt cgtccggcag tctccttgct ctagggagcc actcataaac
     9241 gcaccggcga ttaagggttt tactgctgca cagggatcgg cggcagtttt cgcttccagc
     9301 tggaatgagc ctagggtgcg aacctctttg gtggtgacct gcacaaggta atcccccgcc
     9361 ttttgcacag aataactacc gctggcggga atgcgcgcgc cattgccgtc accgttgttg
     9421 ctcagcaagg tacggcccct ggcatctttc agcgacagca ccgaatcaaa ggttgcgccc
     9481 agagcaaaca ccaccggttg gccgggggtt aaatgcacta tgtagttttg gaaggagcgc
     9541 aagttctcct ctggccccat taaacagggg ccagagtgca attcaccttt gaccactgaa
     9601 ttgagtttga gcagttggtt acagccagcc gcgcccgaac tggagcttga ggttgaagtg
     9661 cttgaggagg tggacgagcg gccggagctt gaagaggagc ttgttgaact gcttgagcca
     9721 ccgaaacccg atgatgaggt ggatgaactt gcccagccac cggatgaact cgccaagcca
     9781 ctggagctgg aggatgaatt gccccaaccg cttgagctgg tactgctgga ggagttgctc
     9841 caccagccac tgctgcttga tgtgcttgtg gacgtgctgc tggagcttga gcttgagctg
     9901 gaagacgagc tggttaaacc gctggatgag ctgctggaag tggtgccctg ccaatttaaa
     9961 ttggtgaacg tgaaattaaa cgctccccag gtgtagggca atgcggtggt gagttctaac
    10021 gcgtatttcc cggctggcaa tttaaggtaa ttttcagcgg gaacgcgtag gggtttatta
    10081 acctcacttt gggcttggag cagcttctcg ccctgctcgt tggtcagggt tagataaggt
    10141 gcgaagtcgg cactcgcaag atccagagca atatccgatt cctcaggaag gtcgaggctg
    10201 aaatgaatgt aaaaacgatc agtgcctaag ctgccggagg agcaggtaat gggtagaaac
    10261 aagcgttcat ttattggtgc cagtctccaa attgatgagc atttagcggc cagagtttct
    10321 tgatcaataa gggccggagc aggattgctg gtaacgacgt taataaaata ctggccgctt
    10381 accgcaggat caacaaacgc cacttcgagg ctataggttc ctgcctcaag ggcaagcata
    10441 gtgcctttgg cgggaatgct cgcgtaaccg ccgctggatt cgccgcgtgt aacgccctgg
    10501 ctatcgagaa ctttaatgtg cacgggcgta acgccgatgt cgtaagaaaa cgcaatgtgt
    10561 tggccctgtt gaagggtaaa tgtgaagcgg tcaaataaac tttgcggatc gttgggctgg
    10621 ctgcaatccc cttcggcaat ggcacccgca acgctttggc tgctgctgtc atgaataaag
    10681 agatcttgtg gttcgcagac gcccccggat gaactgtctt cagcaccgcc ggaagaactt
    10741 gatgagctgc tgctggatga gttactgggg gtaagcgcgt gggttaaatt agcgcaaaaa
    10801 aatgatgcgc ttaataggct ataacaggcg agcaaactaa attttggcca gctgcgctgc
    10861 gccagtttag agtgtgagta catgtgattt tccttcgatt gatatgagcg tctgcgaatt
    10921 gatcagacaa gaagaggcaa tgttcactca aaaacctcgc gatcattaca cctgtgaagc
    10981 acttattggc gcgggctatc cgtcacaaaa agcggtctaa ctactgcgca tttttaacat
    11041 tcatcaagct tttacctggg gaatctccgc ttgtctccgc ttgttaatgg ggcgcaagtc
    11101 ataaatgcct gccattgcag gcatgctgtg catacatcca ttgtgaattt gccaaaaaat
    11161 tcctcttttt gacggcccga aatagcagct attcattccg ggtggctaag cagtcaattg
    11221 aaggcaggac tattgtgaag ccctcagggg tgtgaaacct ttgcgggcgc aagcacgcgc
    11281 cttaggctac acagatgcac ctgtgtattg tcaactcgtt gcaaatgcag gtttaattag
    11341 agcattctgc gtgggtgcct gaattagcca aaaatttttt tgccggtgcc gtaggtgctg
    11401 aatattcacg tgtgaattgg gcgccccgta tttatcttga ggatttacgg cgcgcaaaaa
    11461 cgctcgccaa gggttttgat attgtgcata tataaggggt tttatgagta aattaataat
    11521 gggtttggat ggcaagcagc gctgcagttg gtgttcgagc gtcacggaat ttatccccta
    11581 tcacgataag gagtgggggt ttcccgtggg cgatgatgtg cgtttatttg aaaaactctg
    11641 tttggagagt tttcaatcgg gcctgagctg gcggacaatt ttggccaaac gggagaattt
    11701 ccgcgccgca tttgcaggct tcaattttaa taaggttgcc cgctttaccc aaaaagacat
    11761 aacccgctta ctgcaggatg aaggcatagt gcgccaccgg ggtaaaattg atgcggtgat
    11821 taataatgca cagtgcgcag ttgatatggt taaaaaggaa ggctccttag ccgcttattt
    11881 ttggcgtttt gcgcccaaaa cccatccggc tgaaatagcc caaactcagt ccacgtctgt
    11941 tgaatccatt gcattatcca aagaactaaa aaaactcggc tggaaatttg tggggccgac
    12001 taccgtgttt gcgtttatgc aggcaatggg gttgatcaat gatcatgtgg aggggtgttg
    12061 ggtgcgttcc gaggtagaaa aggctcgtaa aaaatttgtg cagcctaaat aattgagtaa
    12121 gtctattcga gatttgggaa ttttcgtttt taactgaagc tgcaattcat tttgtggcag
    12181 ccccgatcaa ggggcgtgga tgctcgggat ttgaactgcg gaatcctgga taaaatagat
    12241 tttttgtttt cttgggcaag tttaaaaata aatttctgtc cctggtgtta aggggtggca
    12301 gctgcgtggc tttaaagttc aaagccagat tggagtgaga aaaatagtag tggcaagagc
    12361 caaaaaatag ggcgctaggc ttattgtcgc atatcttgtt aattggatat aatccgaaat
    12421 gctttcgggc cgctcaatag acaaatgaac caaaagtaat gtatttcttt gcggtcaaac
    12481 agagagtctt atggatagga acttgtatta ggcctgctat ccggtgcaat agaaatcagg
    12541 gcgtgtacgg tggtgcaggc taaggattaa tatgtggccg cagcaaaaag cacacgctca
    12601 ttttaatagc agcgcagccg cacgccatgg ggaacatctt caatgccaat cattttttgt
    12661 agccaattgg cgctgtaaac ggattttttt tcatagacag gagtgccgag acgttcttct
    12721 attttgttat agtcgtgata aacgacacat tcattgggtt tgggggttac ctcatcgccc
    12781 tttatttcaa agggaatgct ctttggattg tcaataaatt caagaaaaaa acgcagattt
    12841 gcataggatg cccccgtaaa ataaatttta tcgggagctc gatggactgc ttcctgtagg
    12901 cgcgataacg aatcgtaatc ttcggttgct tttaagctgc gcccaggata aacgacaaaa
    12961 taagcaaata gaaaacacgc gacttcaatt gagagaatcc acagtaggcc tttgcttatc
    13021 cggagtgaaa atttgctcgt gtgctgcaat aaaaagttga agccataaca agcgagatag
    13081 gccatgtaaa ccggccccag cattcccctg agcgcgtgag cgctaccgtt agtcagcgcc
    13141 gcggcaaagg gtgcgaacgc gaggttaatg actaaaaggc gtaaaaaaat acgttgccta
    13201 tcatgtgttt ttaagaatgt gaatatgcca atgatagcta atataaatgt tgtgaaaaat
    13261 atctcgcccg catagccgat ggcgtggcga agattctcat cgccttttag aagtaaaaat
    13321 tcgaaggaga aatatttacc ataattatgt acaaaatggc tcagcttcgc cagggtggaa
    13381 atatcatcgt caatgaatga aagttccttg aagcgagtgg tcaagcctgc agagttttca
    13441 aatgcaaaat aaccataggg tatcaaagcg acaaggcagc caagtgcaat agtggg
//
LOCUS       DAOQGS010000034        13385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2149_length_13385_cov_4.685446, whole genome shotgun sequence.
ACCESSION   DAOQGS010000034 DAOQGS010000000
VERSION     DAOQGS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13385
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_13385_cov_4.685446"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKZ52_02280"
     CDS             complement(<1..231)
                     /locus_tag="PKZ52_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58623.1"
                     /translation="MLAKRMRPLLGCFVEIALPVAADDAIFTAAFAQIELIHQLLSFH
                     SSESDLTRLNRANGKPVLCHPLSLQCLRLARAI"
     gene            complement(240..791)
                     /locus_tag="PKZ52_02285"
     CDS             complement(240..791)
                     /locus_tag="PKZ52_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNG58624.1"
                     /translation="MRNVPFFIMIPALAAVTSTAGHYVYAEDYLTVQQAQQSLFPGAT
                     GFSEAPIALSDAQRDEIKKIAGVGQRQAVQKTWRAEKNGELLGWFMVDDVIGKHDFIT
                     YATAISAQGGVLGVEILSYRETHGDQIRQSQWRQNFTGKTLADGFKLGKDIPNISGAT
                     LSCRNVTDGVKRLLAIYQIALKA"
     gene            complement(1008..1898)
                     /locus_tag="PKZ52_02290"
     CDS             complement(1008..1898)
                     /locus_tag="PKZ52_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58625.1"
                     /translation="MSTHTLRASLLAACFWSAAAMADENLFGYVKGSEVLPQGAIEFY
                     QVVTQRSDKGVGHYTAYNTTTELEYGVSDRFTWAGEIKGQQIDTSGLLIDGYMPGDEN
                     YGLRYAGIETSAKYNFLSPALDDIGLSALLGFEYLTLDPHSGRKKDSISISLDFQLQK
                     YFLEGQVVWVANVGTEGTRATRAPIDNLPEDFEWPTAMEVEVEFMAGTGVSYRFAPSW
                     FVGLEALYETEYETEVAMERQSLFAGPSLHYGGEKYWLTLTWMQQIEGGGETFEGQPD
                     GLHLIEKTKHEARLKLGLNF"
     gene            2102..2518
                     /locus_tag="PKZ52_02295"
     CDS             2102..2518
                     /locus_tag="PKZ52_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNG58626.1"
                     /translation="MEDIIEKLRANAQDVPVPLDLPDEDQLLDIEEALLTPLPKSLRA
                     FLLTVSDLVIGSLEPATAADPHAHTYLADMAADAWERGLPRHLLPFCQSGSLYYCINL
                     EDAVVVWSPASGESDAEWEDIWHWAEAVWLPSGQRA"
     gene            2731..4584
                     /locus_tag="PKZ52_02300"
     CDS             2731..4584
                     /locus_tag="PKZ52_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58627.1"
                     /translation="MSFLAEHLFSILAISLLAAFSVASGVQRLRLQRKLKSLQAAPDK
                     HSIITHFLNEQDSKTQRLKNTSLLAMPGLNKKALRMRHAYLKIESRMLESHTYNSRDY
                     YIGLVTHLTQLLKILDGDHEHQEEIANSLHQAAESIQLSASERAEIIECIETLLQRAH
                     SAQLDSAELHALGKRVLAILKQQDDPKQRQRTAKAYALSELQKKTLQQTDQLKHVAHT
                     NRNHNQQLISRWEDTNNAAQLLEKAREFEQENHRLSMQISLLQHELNSSRRSAFGQTQ
                     EDVQEAASLADQLIEFNEQEIRKLKNTIRSQKKIIFDLQESLEKHTSISPQEREEYTE
                     QLAQLQRNLAQAETCIEMLEQELAQLRERFNSVKHLEEAAQSEALKAQLSAITEAIKP
                     ATTEASQQKNELAFIQEAIAADSLEDLSLLIYQYYLDQQHNPVLLITHKNRRIEMCGN
                     GNFSPKQKNSLAGLRPNESTYDRSSGQLHFQYQYIGGILTLPPNSSAEQENALKIARI
                     ADRVIDKIMANHANRQLKKQIEAGSNIAKQLARDIENAYDQQINTCKTVIFDNFKNIQ
                     NLLQSKNATPTQIASVKQSEQDILEELMANNSYRIKLRREFINLVKKIEDK"
     gene            complement(4651..4851)
                     /locus_tag="PKZ52_02305"
     CDS             complement(4651..4851)
                     /locus_tag="PKZ52_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58628.1"
                     /translation="MAKSKCPHCQHEAFELVERDDIKDSKFKIMFIQCVACATVVGTT
                     DFYNVPSLLEKLARKLGVELHD"
     gene            complement(4937..7033)
                     /gene="fusA"
                     /locus_tag="PKZ52_02310"
     CDS             complement(4937..7033)
                     /gene="fusA"
                     /locus_tag="PKZ52_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNG58629.1"
                     /translation="MTDLSTYRNIGIFAHVDAGKTTTTERILKLTGRIHKIGEVHEGE
                     STTDFMVQEAERGITIQSAAVSCFWKGHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGV
                     FCGSGGVEPQSETNWRYANDSKVSRLIFVNKLDRIGADFLRVTEQVKKVLGAKPLIMT
                     LPIGREDTFVGVVDLLNRKAYVWDESGQPENYSVQDIPADMVDDAEMYRDQLVETAVE
                     MDDDLMMAYMEGEQPSIDDIKRCIRKGTRELAFFPTYCGSAFKNKGMQLLLDAVVDYL
                     PAPHEVNPQPLTDEEGNPNGQYAIVSADEPFRALAFKIMDDRFGALTFVRIYSGKLAK
                     GDTILNSATGKSERVGRMVEMQANDRIELSNAEAGDIIAIVGMKNVQTGHTLCDPKHE
                     CTLEAMVFPEPVISIAVAPKDKGSTEKMGIAIGKMVAEDPTFRVETDQETGETILKGM
                     GELHLDIKVDILKRTYGVELVVGQPQVAYRETITREVEDSYTHKKQSGGSGQYGKIDY
                     RIRPGEANTGFTFKSSVVGGSVPKEFFPAIEKGFKSMMDTGPLAGFPVLDVEIELFDG
                     GYHAVDSSAIAFEIAAKSAFRQSMPKAGPQLLEPIMKVDVYSPEDNVGDVIGDLNRRR
                     GMITSQEANITGVRIKANVPLADMFGYISTLRTMTSGRGQFSMEFSHYSPCPNNVADA
                     VIAKEKEKKAAAAKNK"
     gene            complement(7304..8185)
                     /locus_tag="PKZ52_02315"
     CDS             complement(7304..8185)
                     /locus_tag="PKZ52_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNG58630.1"
                     /translation="MLDALALLIIKALGHLPIAWARGLGAGLGRLALLFNIPMVRVTR
                     RNIAYCLGHLPPPEQARLVRASVLETARLAVESTVIWRCERATLDPLIKAVHGREHVE
                     EAKAAGRGVIILAPHLGNWEVLGRYLDRLGEMTLMFQPQKLPKVSDYIKAGRGRFGAT
                     LVATDRRGLAQMVAALRKGGLSGVLPDQTPRDDNSGLLAPFFNRPAFTMTLVCKLIQN
                     TGCGAVFGYAKRVPGGFEIYFNRAPAGLFSEDLAEAVAALNQGVSEAVLQVPEQYQWE
                     YKRFKHNGEKTLYDNLV"
     gene            8391..9359
                     /gene="glyQ"
                     /locus_tag="PKZ52_02320"
     CDS             8391..9359
                     /gene="glyQ"
                     /locus_tag="PKZ52_02320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNG58631.1"
                     /translation="MTPAPDVSTFQGLILALQQYWAEQGCVILQPLDMEVGAGTFHPA
                     TFLRAIGPETWNTAYVQPCRRPADGRYGENPNRLQHYYQFQVILKPSPSNIQDLYLGS
                     LQRLGLDTQIHDVRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECYPV
                     SGEITYGLERIAMYLQNKESIFDLVWTINPQGEPVTYGDVFHQNEVEMSHFNFTHANV
                     DFLFQSFDVYEAEAKRLMGQNLPLPAYEMVMKASHAFNLLDARQAISVTERQRYILRV
                     RTLAREVAQAYFDARLALGFPLAPAALRDEVIARVTAERKTQEVNA"
     gene            9359..11476
                     /gene="glyS"
                     /locus_tag="PKZ52_02325"
     CDS             9359..11476
                     /gene="glyS"
                     /locus_tag="PKZ52_02325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNG58632.1"
                     /translation="MSSHQFFVELGTEELPPKALLSLQEAFATHVQDALKAAGISFSE
                     LKAFASPRRLALRIEGLAANTPRQALTHWGPPAKMAKDEAGNPSKAALAFADKLGVSF
                     DQLQVANDGKIDKLSFQAEVGGEPVRDLMAGFISAALAALPIPKRMRWGSSRNEFVRP
                     ALWLVMLWDNQVLPAQILGLNAGNSTRGHRIHAPNPITLSEASQYETVLKNDGFVLAD
                     FAARRSLIVELVNAEASKLGGTAVIDADLLDEVTALVEWPVALAGRFEERFLSVPAEA
                     LVSSMKEHQKYFHLVDAKGALLPYFITLANLASRDPTQVIAGNEKVIRPRLSDAAFFF
                     TTDCQTPLATQREKLKTVMFQAQLGSMFLKSERVAHLAGVMADLLGWDVRLAQRAGEL
                     SKADLVSKMVYEFAEMQGIAGYYYALNEGEPQEVAQAIKEQYLPRFAGDELPKSHTGC
                     LLALADRLDTLVGIFGLGQIPTGSKDPFALRRASLGVLRILVEKRLDLDLKVLLQEAI
                     DQHKLPRPGGNISREVVVTLGPRADTLDTALAYILERLASVYSDQGISPEIYQAVSAK
                     ALSNPLDIHHRIQAVAAFSQLPQAASLASANKRVSNILAKLPEAPADAINPSLLQLPA
                     EQALAAALSQAQADVGPLLQDGEYQTALTQLASLQAPVDAFFTDVMVMADDLALRNNR
                     LALLQQLRDLFWQVADISCLVVK"
     gene            11490..12242
                     /locus_tag="PKZ52_02330"
     CDS             11490..12242
                     /locus_tag="PKZ52_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNG58633.1"
                     /translation="MLTWLRSALFSIVYIPSTVFYGAATQLVRWLPPSILPPAKLHRF
                     VITWCRLNVEWLRITVGARYQVSGLEHLQNLPGPVMVLAKHQSTWETLFLQLLFFPAI
                     TVLKRELLSIPFFGWGLRGLRPVAIDRSNPRDALKLVKERGLEQLAAGNNMLLFPEGT
                     RTQLGERGKYARSGADIAISAGVPVIPVALNSGKCWPPKQLRKYPGLISVVIGAPIPT
                     EGRTSKELIEQVENWIEGELARIAAEEAQAGR"
     gene            12402..13307
                     /locus_tag="PKZ52_02335"
     CDS             12402..13307
                     /locus_tag="PKZ52_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HNG58634.1"
                     /translation="MAPSKSLLLSLGFSLSASFAHASLLDYNLALQNNLTGAVHVEGM
                     SFIGGNLTATQLSEFNSGNDTPVGTFDGLEVGGKVSGQVKILNGETAVYKTKDAAAQI
                     QCVGSVNCATGGVDLTAKKQALTSEMSSLSSSYKALSSNASAVKNGNQIKLQYTGSDS
                     LAVFNLTASDLFFQNSSIELALGSASKAIINVSGNLSVSNTNLTGAWNYSNTLWNFYD
                     ATSLNFNAMAVKGSVLAFGADVFDGGGFDGSLYAKSYTNSSLREIHGFFWTPPTTNTV
                     PEPEMFGLMLGGLGLLGLARLRRRA"
BASE COUNT         3178 a         3844 c         3464 g         2899 t
ORIGIN      
        1 gatggcgcgg gccaaacgca ggcattgcag gctcaatgga tggcagagaa ccggcttgcc
       61 gttggcgcga tttaagcgcg ttaaatcact ctcagaggag tgaaaactta acagctggtg
      121 gattaattca atctgcgcaa atgcggcggt aaaaatggcg tcatccgccg ccactggcaa
      181 ggcaatttcc acaaaacacc ccagcaacgg gcgcatgcgt tttgcgagca tcggctccat
      241 taagccttta aagcaatctg ataaattgcc agtaagcgtt taacgccatc ggtgacgttg
      301 cggcaggaca aggttgcgcc actaatattg ggtatatctt tgcccagttt aaaaccatcc
      361 gcgagggtct taccggtgaa attctgccgc cactgactct ggcgaatttg atcgccgtgg
      421 gtttctcgat aactgagaat ttccaccccc aacacaccac cttgagccga aatcgccgtg
      481 gcgtaggtga tgaaatcgtg tttacctata acatcatcca ccataaacca gccgagcaat
      541 tcaccatttt tctccgcccg ccaggttttt tgcaccgctt gccgctgccc aacaccggct
      601 atttttttta tttcatcccg ctgtgcgtca ctgagcgcaa taggcgcctc actaaaacct
      661 gtggcgccgg gaaataggct ttgctgagct tgctggaccg tcaaataatc ttctgcgtag
      721 acatagtgtc cggcagttga tgtcaccgca gcaagcgccg ggatcataat aaaaaacggc
      781 acattgcgca taaaatctcc tccgcaaatt gagtgtgtag gctgctgtaa ttaacagcct
      841 cttaaaatat ctgcctgcaa aaaacacacg gtctttaaac aggcaattaa tttggccttt
      901 tgtgtggcat tagggtaatg gcggcaactt aagcgcaagg gcgacaactt aagtgaatgg
      961 ccagtgcaaa attgttccgg ccgcacaaaa tccgcctgga tgcgccacta aaaatttaat
     1021 cccagtttca agcgcgcttc atgcttggtt ttttcaatta aatgcaagcc gtcgggctgt
     1081 ccctcaaagg tttcgccacc gccttcaatt tgttgcatcc aggtgagcgt aagccaatat
     1141 ttttcgccgc cataatgtaa gctcggcccg gcaaataaag actggcgctc catagccact
     1201 tctgtttcat actcagtttc atagagcgct tcaaggccga caaaccaact gggcgcaaag
     1261 cgataactca cgccggtgcc cgccataaat tcaacttcaa cctccatggc ggtgggccat
     1321 tcaaaatcct cgggcaaatt atcaatcggt gcccgcgttg cccgcgtacc ttcagtgccc
     1381 acattagcca cccagacaac ctgcccttcg agaaaatatt tctgcaattg aaaatccagg
     1441 gagatactga tggaatcttt tttgcggccc gagtgtgggt ctagggttaa atactcaaac
     1501 ccgagcagtg cagaaaggcc aatatcatcg agcgcagggc ttaaaaaatt atattttgcc
     1561 gaggtctcaa ttcccgcata gcgcaggcca tagttttcat cgcccggcat ataaccatca
     1621 attaataaac cggaagtatc aatttgctgg cctttaattt cgcccgccca ggtaaaacga
     1681 tcgctaacac cgtattccaa ttcggtggtg gtgttgtacg ccgtgtagtg gccaactccc
     1741 ttatcgctgc gctgggtcac cacttgataa aattcaatcg cgccttgcgg cagtacttca
     1801 gaacctttta cataaccaaa caggttttca tcggccatgg ctgcagccga ccaaaaacag
     1861 gcggccaata aggatgcgcg cagagtatga gtagacacaa taatctcacc ttaggaatca
     1921 taaacagttg ccagactatc acaggcgatg ttcgatttca tatgatacct attctcattt
     1981 gatccatcgc aaacttcttc gcccttattt actctgccct gcctggggca aagtccctgc
     2041 gggggtggta catttggcct gcctcaacca ttgccaacta acccagtaaa gagaacaacc
     2101 cgtggaagac atcatcgaaa agctgcgcgc caacgcgcaa gacgtgccgg tacccttgga
     2161 tctgcccgac gaagatcagt tattggatat cgaagaggcc ctgctcaccc cattgcccaa
     2221 aagcctgcgg gcatttttat taaccgtcag cgatttggtc atcggcagct tggaacccgc
     2281 caccgccgcc gacccccacg cccataccta tcttgccgac atggccgctg atgcctggga
     2341 gcgcgggctg ccccggcacc tgctgccttt ttgccagagc gggagcctgt attactgcat
     2401 taacttagag gacgcagtgg tggtctggag ccctgccagc ggggagtccg acgccgaatg
     2461 ggaggatatt tggcactggg cagaggcggt gtggttgccg tctggacagc gcgcttaggg
     2521 gcctgccaag cagagcaggt cgtgagtttg aactacactt ggggagtatg gcgcccgcaa
     2581 ggcgccgcct ggcctgttgc cgcgtagtgg gtgaaacagc gggcaggctt cgcaattgta
     2641 ctcaaccgag gtatttagga ttatgcgcgc cagcccacgc ccgcccgccg caaggctcgc
     2701 aaccgtcaac accgttaagc caaggccacc atgagtttct tggcggagca tctatttagc
     2761 atcctcgcca tcagcctgct ggccgcgttc agcgtggcta gcggggtgca gcggctgcgc
     2821 ctgcagcgca aactcaaaag cctgcaagcc gccccggata aacacagcat catcactcat
     2881 tttttaaatg agcaggacag caaaacccaa cgcttaaaaa acaccagcct gctggccatg
     2941 cccggcctta ataaaaaagc cctgcgcatg cgccatgcgt atttaaaaat tgaaagccgc
     3001 atgctggaga gccacaccta taacagccgg gattattaca tcggcctagt tacccattta
     3061 acgcagttgt tgaaaattct cgatggcgac cacgaacacc aagaagaaat tgccaacagc
     3121 ttgcaccagg cggctgaatc tattcagtta tcagctagtg agcgcgccga aattatcgag
     3181 tgtattgaaa ccctattgca gcgcgcccat agcgcccaat tggacagcgc cgaactgcat
     3241 gctctgggca agcgagtgct ggccattctc aaacaacagg acgaccccaa acagcgccaa
     3301 cgcaccgcga aagcttacgc actaagcgaa ttacagaaaa aaaccctgca gcaaaccgac
     3361 cagctaaaac acgtggcgca caccaatcgc aaccacaatc aacaattaat cagccgctgg
     3421 gaagacacca acaacgccgc gcaattattg gaaaaagccc gggaatttga gcaggaaaat
     3481 caccgcctga gcatgcaaat tagcctatta caacacgagc ttaacagcag ccgccgctcc
     3541 gcgtttgggc agacgcaaga ggatgttcaa gaggcggcaa gccttgccga tcagttaatt
     3601 gaatttaatg aacaggaaat acgcaaactc aaaaatacca tccgttcgca gaaaaaaatt
     3661 atttttgacc tgcaggaatc cctggaaaaa cacacctcca tcagccccca agagcgcgag
     3721 gagtacaccg agcaattggc gcaactgcag cgcaatctgg cacaggcaga aacctgcata
     3781 gaaatgctgg aacaagaatt agcccaattg cgcgagcgct ttaatagcgt taaacactta
     3841 gaagaggccg cccagagcga agccctcaag gcgcagttaa gcgctattac agaagcaatt
     3901 aaacccgcca ccaccgaggc ctcgcagcaa aaaaatgagc tggcatttat ccaagaagcc
     3961 atagcagcgg attcgctgga ggatttatcg ctactgattt accagtatta tttggaccaa
     4021 caacacaacc ccgtgctatt aattacccac aaaaatcgcc gcatcgaaat gtgtggcaac
     4081 ggcaacttta gccccaaaca aaaaaacagc ctggcaggcc tacggcccaa cgaatccact
     4141 tatgatcgca gcagcggcca gttacatttc caatatcagt atattggcgg catattaacc
     4201 ctaccgccca actccagcgc cgagcaggaa aacgccctca aaattgcccg cattgccgac
     4261 cgcgtcatcg ataaaatcat ggccaaccat gctaaccggc agctgaaaaa acaaattgaa
     4321 gcgggcagta atatcgcaaa acaactggct cgggatattg aaaatgccta cgaccaacag
     4381 attaatacct gtaaaaccgt cattttcgac aactttaaaa atattcaaaa cctgttgcaa
     4441 agcaaaaacg ccacccccac acaaattgcc agcgttaaac agtccgagca ggatatttta
     4501 gaagagctaa tggccaacaa tagttaccgc atcaagctgc gccgagaatt tattaatttg
     4561 gtaaaaaaaa tcgaggacaa ataacgccgc tctcgcctct gcccctgccc gtttgcgcac
     4621 cgctcgggtt taaacctaaa aaccgcaggt ttaatcgtgc aactcaacgc ccagcttgcg
     4681 ggccaacttt tctagcaaac tcggcacatt gtaaaaatct gtagtgccca ccactgtggc
     4741 gcaggcgacg cactgaataa acataatttt aaatttggaa tccttaatat catcccgctc
     4801 caccaattca aacgcctcat gctggcagtg ggggcatttt gacttagcca tacaaacacc
     4861 tcttctggta aaaaaataac ggacaaaaaa aagccccggc gcgcaggcga cggggcttag
     4921 cttaacctag gcgctactac ttgtttttag cagcggcggc ttttttctct ttctctttgg
     4981 cgatgaccgc atcggctacg ttgttcggac atggtgagta gtgcgagaac tccatggaga
     5041 actgaccgcg accggaagtc atggtgcgca gggtgctgat gtaaccgaac atatctgcca
     5101 ggggaacatt agccttgatg cgcacgccag tgatgttggc ttcttggctg gtgatcatgc
     5161 cgcggcgacg gtttaagtca ccgataacat cacccacgtt gtcttctggg ctgtacacat
     5221 ccactttcat gatgggttca agcagttgtg gaccagcttt aggcatggat tgacggaagg
     5281 cgcttttggc ggcgatttca aacgcgatgg ccgaggagtc tacggcgtgg tagccaccgt
     5341 caaacaactc aatctccaca tctaatacag ggaagcctgc caaggggccg gtatccatca
     5401 tggacttaaa gcctttttca atggcgggga agaattcttt tggaacgcta ccacccacaa
     5461 ctgaagactt aaaggtaaag ccagtgttgg cttcgccggg gcggatgcgg tagtcgatct
     5521 taccgtactg gcccgaacca ccggattgtt tcttgtgggt gtagctgtct tccacttcgc
     5581 gggtgatggt ctcgcggtag gctacctgcg gttggcccac taccagttcc acaccatagg
     5641 tgcgctttag gatgtccact ttaatatcca agtggagttc acccataccc ttaaggatgg
     5701 tttcgccggt ttcttggtcg gtttccacgc ggaaggttgg gtcttcagcc accatcttgc
     5761 cgatggcgat acccattttc tcggttgagc ctttgtcttt aggcgcaacg gcgatggaga
     5821 tcaccggctc tgggaacacc atggcttcca gcgtacattc gtgtttcgga tcacacaagg
     5881 tgtggccggt ttgcacgttt ttcataccca cgatggcgat aatgtcgccc gcttcggcgt
     5941 tggataattc aatacgatcg ttggcttgca tttccaccat gcggcctaca cgctcggatt
     6001 taccggtggc ggagttcaag atagtgtcgc ctttggccaa tttaccggag taaatacgca
     6061 cgaaggtgag ggcgccgaaa cggtcgtcca tgattttgaa cgccagggcg cggaagggct
     6121 cgtcggcaga cacaatggcg tattggccat tggggttgcc ttcttcgtcg gtgagcggct
     6181 gtgggtttac ttcgtgtggt gcaggcaggt aatccactac cgcatccaac aataactgca
     6241 tacctttgtt tttaaaggcc gaaccacaat aagtggggaa gaaggccagc tcgcgggtac
     6301 ccttgcggat gcagcgcttg atgtcgtcga tggagggctg ctcgccttcc atgtaggcca
     6361 tcattaaatc gtcgtccatc tcaacggcgg tttccaccag ttgatcgcgg tacatttcgg
     6421 catcgtcaac catgtcggca gggatatctt gcacactgta gttttcaggc tggccggact
     6481 catcccacac ataggctttg cggtttaaca ggtcaaccac gccgacgaag gtgtcttcgc
     6541 ggccgatggg caaggtcatg attaagggct tggcgccgag gacttttttc acctgctcgg
     6601 ttacgcgcag gaagtccgcg ccgatacggt ccagtttgtt tacgaagatc aaacgggaca
     6661 ctttagagtc gttggcgtag cgccagttgg tttccgattg cggctcaaca ccgccggagc
     6721 cacagaatac gccgataccg ccgtccaata cttttaagga gcgatacact tctacggtga
     6781 agtcaacgtg gcccggggta tcgataacgt taaagcggtg gcctttccag aagcagctca
     6841 cggcagcgga ttgaatggta atgccgcgtt cagcttcctg caccatgaag tcagttgtgg
     6901 attcaccttc gtgaacctca ccaattttgt gaattcgacc ggtcagctta agaatgcgct
     6961 cggtggttgt ggtcttgccg gcatctacgt gggcgaagat acctatgttt cggtaggttg
     7021 ataagtcagt catagcactc tctataagct ggggtgaaaa ttgggcagca caatacacga
     7081 aatcgtgcca atttgcccaa atattgcgaa gcacctgggt aaaggctggc ttttttctcg
     7141 aattatgccg agttagccgc gtttgttaac cgcgactggg cacagtgtgg aggggttttg
     7201 cgacaaaacc cggacaggcc tgtaatccgg gtgagcgcat ttaagcagat tgcgcgccct
     7261 aaggtgggtt tatcttccct accccgcccc caacctagtc gcgctaaacc aggttgtcgt
     7321 agagggtttt ctcgccattg tgtttaaagc gcttgtactc ccactgatat tgctcgggca
     7381 cttgcagcac cgcctcactc accccttggt taagtgctgc caccgcctcg gccagatcct
     7441 cactgaagag gcctgcgggg gcgcggttaa aataaatttc aaagccgccg ggcacgcgtt
     7501 tggcgtagcc gaacactgcg ccgcagccag tgttttgaat gagcttgcag accagcgtca
     7561 tggtaaaggc cgggcggtta aaaaacggcg ccagcaaacc gctgttgtca tccctggggg
     7621 tttgatcggg taacacccct gaaagcccgc cctttcgcag ggccgccacc atctgggcca
     7681 ggccgcgccg gtcggtggcc accagggttg cgccaaagcg gccgcggcca gcttttatat
     7741 agtcactcac cttgggcaac ttttgcggct ggaacatcag ggtcatctcc cctaatcgat
     7801 ccaagtaacg gcccagcacc tcccagttgc ccagatgagg ggcgaggata atcacccctc
     7861 ggcctgcggc cttggcctct tcaacatgct ctcggccgtg cacggcttta atcagcgggt
     7921 ccaaggttgc gcgctcacag cgccaaatca ccgtgctctc caccgccagg cgcgctgtct
     7981 ccagcaccga ggcgcgcacc aggcgcgcct gctcaggggg tggcaaatgg cccaggcaat
     8041 aggcgatatt gcgccgggtg acccgcacca tggggatgtt aaacagcagc gccaagcgcc
     8101 ccaggcccgc ccccagcccc cgcgcccagg cgatgggcaa atggccgagg gctttaatga
     8161 tcagcagggc taaggcatca agcatagggt taaggtatcg agcatagagt taaggccagc
     8221 acaaaaaggc gccattgtgc caagcgtgtg ggttttggca acgggctttg tgaaggaatg
     8281 ggtgtagggc tttcttggtg aatagatccg aaccccctat aatcgccgcc tttttatctt
     8341 ttggccgccg caaggccttt atgtaccgct tttaacccca ggagggttta atgacgcccg
     8401 ctcccgatgt atcaacattt caaggtctca tcctcgccct gcagcaatac tgggccgaac
     8461 agggctgtgt gatcctgcag ccgctggata tggaagtggg tgcaggcacc tttcacccgg
     8521 ctaccttttt gcgcgccatt gggcctgaaa cctggaacac cgcttatgtg cagccctgcc
     8581 gtcgccccgc cgatggccgc tacggtgaaa accccaaccg actgcagcat tactaccagt
     8641 ttcaggtgat tctcaaaccc tcccccagca atatccaaga cctctaccta ggttccttac
     8701 agcgcctagg gctggatacc cagattcacg atgtgcgctt tgtggaagac aactgggaat
     8761 cccccaccct cggtgcttgg ggcttaggct gggaggtgtg gctcaatggt atggaagtga
     8821 cgcaatttac ctacttccag caggtgggtg gcctggagtg ttatccggtg agcggcgaga
     8881 tcacctatgg cctggagcgc atcgccatgt acctgcagaa caaagaatcc atttttgact
     8941 tggtgtggac gattaaccca cagggtgagc cagtcaccta cggcgatgtg ttccaccaaa
     9001 acgaagtgga gatgagccac ttcaacttta cccacgccaa tgtggacttt ctgttccaaa
     9061 gctttgacgt atacgaggcg gaagctaaac gcctgatggg ccaaaacctg ccattgcccg
     9121 cctatgaaat ggtgatgaag gcgagccacg cttttaacct gctcgacgcc cgccaggcca
     9181 tctccgttac cgagcgccag cgctatatcc tgcgggtgcg caccctggcc cgggaagttg
     9241 cccaggccta cttcgacgcc cgcctggccc tcggcttccc cctcgctcct gccgctctgc
     9301 gcgatgaagt tatcgcccgt gttactgccg aacgcaaaac ccaagaggtc aacgcctaat
     9361 gtctagccat cagttttttg ttgaactcgg caccgaagag ttacccccca aggcattgtt
     9421 aagcctgcaa gaggcctttg ccacccacgt gcaagacgcc ctaaaagcgg cgggtatcag
     9481 ctttagtgag ttaaaagcct tcgccagccc ccgccgcttg gccctgcgca ttgagggact
     9541 ggccgccaat accccacgcc aagccctcac ccattgggga ccaccggcca aaatggctaa
     9601 ggacgaggcg ggcaacccca gcaaagccgc cctcgccttt gccgacaaac tcggcgtgag
     9661 ctttgaccaa ctgcaggtgg ccaacgacgg caaaatcgac aagctcagct tccaggccga
     9721 agtgggcggc gagccggtgc gggatttaat ggccgggttt atcagcgcgg cgcttgccgc
     9781 tctgcccatt ccaaaacgta tgcgctgggg ttccagccgc aacgaatttg tccgccctgc
     9841 tctgtggctg gtaatgcttt gggacaacca agtgctgccc gcccagatcc tgggtttaaa
     9901 cgcaggcaat agcacccgcg gccaccgcat ccacgcgccc aatcccatta cattgagcga
     9961 agccagccag tacgaaactg tgcttaaaaa tgacggcttt gtgctggcgg attttgccgc
    10021 ccgccgcagc ctgattgtgg aactggtgaa tgccgaagca agcaagcttg gcggcactgc
    10081 cgtaattgac gccgacctgc tcgatgaagt caccgccctg gtggagtggc ccgtggccct
    10141 ggccgggcgt tttgaagagc gctttttaag tgtgcccgcc gaagccttgg tgtcctccat
    10201 gaaggagcac caaaaatact tccacctggt ggatgccaaa ggcgcattgc tgccctactt
    10261 cattaccctg gctaacctgg caagccgcga ccccacccag gtcatcgccg gtaacgaaaa
    10321 ggttatccgc ccgcgcttaa gcgacgcggc gtttttcttc accaccgact gccaaacacc
    10381 cctggccact cagcgggaaa aactcaaaac cgtgatgttc caagcccagt tgggcagcat
    10441 gttcttaaaa agcgagagag tggcccattt agccggtgtg atggccgatt tattgggctg
    10501 ggatgtgaga ctcgcccagc gcgcaggcga gctctccaaa gcggatttgg tcagtaaaat
    10561 ggtgtatgaa tttgccgaga tgcagggtat agctggttat tactatgccc tcaacgaagg
    10621 tgagccgcag gaagttgccc aggcaataaa ggaacaatat ctgccacgtt ttgctggcga
    10681 tgaactgccc aagagccata ccggctgcct gctggcacta gccgatcgac ttgacaccct
    10741 agtgggtatt tttggtttag gccaaattcc cacaggctcc aaagacccgt ttgctcttcg
    10801 ccgagcgtct ctgggggttc tgcgcatttt ggtcgaaaaa cggttggatt tagacctaaa
    10861 ggtgttgctg caagaggcta tcgatcaaca caaactgcca cggccaggcg gtaatatttc
    10921 ccgtgaagta gttgtgactt tggggccgcg cgccgacact ctcgacaccg ccctcgccta
    10981 tattctggag cgcttagcca gtgtgtacag cgaccaaggt ataagccctg agatctacca
    11041 agcggtcagc gccaaggcct taagcaaccc cttggacatt caccaccgca tccaagccgt
    11101 ggcggccttc agccagttgc cccaggcggc aagcctcgcc tccgccaaca aacgggtctc
    11161 caatatactc gccaaactgc ccgaagcccc ggccgatgcc ataaacccca gcctgctgca
    11221 actgcccgcc gaacaggccc tagccgccgc cctaagccaa gcccaggcgg atgttggccc
    11281 cctgctacaa gacggcgaat accaaaccgc gctcacccaa ctggccagct tacaggcacc
    11341 agtggacgcc ttttttaccg atgtaatggt gatggcggac gatctggccc tgcgcaacaa
    11401 ccgcctggct ctgttgcaac agctgcggga tttattctgg caggtggcgg atatttcctg
    11461 tttggtggtg aagtgagcct gagtgcccca tgttgacctg gctgcgctcg gcgcttttct
    11521 ccatcgttta tatccccagc accgtgttct acggcgccgc cacgcaactg gtgcgctggc
    11581 tgccgccgag tattttgccg ccggccaagc tgcaccggtt tgttattacc tggtgccgcc
    11641 tcaacgtgga gtggctgcgc attaccgtgg gggcgcgcta tcaggtgagc gggcttgagc
    11701 atctgcagaa tttgcccggc ccggtgatgg tgctggccaa acaccagagc acctgggaga
    11761 cgctgttttt gcagctgctg ttcttccccg cgattacggt gttgaagcgc gaattgctgt
    11821 cgattccgtt ttttggctgg gggctgcggg gtttgcggcc ggttgccatc gaccggagca
    11881 acccgcggga tgccttaaaa ctggtaaaag agcgcggcct tgagcaattg gcggcgggta
    11941 acaatatgtt gttattcccc gagggcaccc gcacccaact cggcgagcgc ggcaaatacg
    12001 cccgcagcgg cgccgatatc gccatcagcg cgggcgtgcc cgtcatcccg gtagccctca
    12061 actcggggaa atgctggccg cctaaacaac tgcgcaagta cccaggcctg atcagcgtgg
    12121 tgatcggcgc tccaattccc accgagggac gcactagcaa ggagttgatc gaacaggtgg
    12181 agaactggat tgaaggtgaa ttggcccgta tcgctgcgga agaggcccag gcaggccggt
    12241 aaacaaacca cccttgcgca ggcttaaagt gtcgtcaaaa ttaacactct tagggccacc
    12301 atcagccaat ttgctattta aatggctgtt attattggca atataaataa gagcacacat
    12361 cctgctaggg tggagctccc gtccacccat gagatgtacc catggcaccg tccaaatccc
    12421 ttctgctaag cctcggcttt agcctttccg ccagcttcgc ccacgccagc ctgctggact
    12481 acaacctcgc cctacaaaac aacctcaccg gtgctgtgca cgttgagggt atgagtttta
    12541 tcggcggcaa cctcaccgcc actcagctct ctgaatttaa cagcggcaac gacacccctg
    12601 tcggtacctt tgacggcctg gaagtcggcg gcaaggtgag cggccaggtg aaaatcctca
    12661 acggcgaaac cgccgtgtat aaaaccaaag atgccgctgc tcagatccag tgtgtgggca
    12721 gcgtcaactg cgccactggc ggcgttgacc taacggcaaa aaaacaggcg cttaccagcg
    12781 agatgagcag cctctccagc agctacaagg ccctcagcag caacgccagc gctgttaaaa
    12841 acggcaacca aatcaaactg caatacaccg gcagcgacag cctggcggta tttaacctca
    12901 ccgcctcgga tctgtttttc caaaactcca gcatcgagct ggccctgggc agtgccagca
    12961 aagcgattat caacgtctcc ggcaacctca gcgtttctaa caccaatctc accggtgcct
    13021 ggaactactc caacaccctg tggaatttct acgacgccac cagcctgaac tttaacgcca
    13081 tggcggtaaa aggctcggtg ctggcctttg gcgcggatgt atttgacggc ggcggctttg
    13141 acggctccct gtacgccaag tcctacacca actcaagcct cagggaaatc cacggcttct
    13201 tctggacacc gcccaccacc aacacagtgc cggaaccaga aatgttcggc ctgatgttgg
    13261 gcggcttggg cctgcttggc ctggctcgct tgcgccgccg ggcataaacg ctcgacataa
    13321 aaaaaccggc ctaggccggt ttttttatgg gcggtattta cgcgggtagc tccagcgccc
    13381 cgcgc
//
LOCUS       DAOQGS010000035        13363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2152_length_13363_cov_4.390216, whole genome shotgun sequence.
ACCESSION   DAOQGS010000035 DAOQGS010000000
VERSION     DAOQGS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13363
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_13363_cov_4.390216"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PKZ52_02340"
     CDS             complement(<1..133)
                     /locus_tag="PKZ52_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNG58635.1"
                     /translation="MSRYALVGNPNCGKTSLFNRLTGANAKVANYPGVTVERRSGFVQ
                     "
     gene            complement(148..453)
                     /locus_tag="PKZ52_02345"
     CDS             complement(148..453)
                     /locus_tag="PKZ52_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNG58636.1"
                     /translation="MQAVTAEYLSPDLSTPVSRLSLTECSKGQHVMLTELRPNPAFGD
                     HDNAVTARMKALGFLPGVQLTVIGYGLFGRDPLAVQINGTKFALRRAEAHKLIVIPL"
     gene            689..1096
                     /gene="flgB"
                     /locus_tag="PKZ52_02350"
     CDS             689..1096
                     /gene="flgB"
                     /locus_tag="PKZ52_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNG58637.1"
                     /translation="MAISFRNSVGVYESALKLRAARAEILAGNLANADTPGYQAKDFN
                     FADALKNQLAGAPNSEDLRLTQPLHQEGLAGAGSGGMDVQFRTPTQPSLDGNTVEEQV
                     EHAEFMQNSLEYQTSFTLLNSRFRGLMSAIKGE"
     gene            1099..1542
                     /gene="flgC"
                     /locus_tag="PKZ52_02355"
     CDS             1099..1542
                     /gene="flgC"
                     /locus_tag="PKZ52_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNG58638.1"
                     /translation="MGLTTIFDIAGSGMNAQSLRLNTTASNIANADTASSSADKTYRG
                     RHPVFAASFPQMLGHPENANAIDSPPESLGVAVKGIVESDAPLQPRYEPENPLADEQG
                     YVYYPNVNVVEEMTNMISASRSYQVNVEVANAAKQMVQKILTLGQ"
     gene            1740..2453
                     /locus_tag="PKZ52_02360"
     CDS             1740..2453
                     /locus_tag="PKZ52_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNG58639.1"
                     /translation="MSDISGLGSAIDALSSANKYKKDEKSPNELGQDAFLQLMISQLK
                     NQDPLSPQDNGEFVSQLAQFSSVEGLDKLNNNFDTFANKFVSNQALQASTLVGRTVTV
                     PGKTTALEPGGIVTTSAELPAGSSNFTLAIYKNNGGLAQTIDFDKVDGDELKVAWNGR
                     DAVVNGKAVTIPESFKGVDPGIYTFKLSALVDGKRTELDNNVTANVNSVTLGKNGTLT
                     LNLAGAGSVSLSDVKEINE"
     gene            2702..5158
                     /locus_tag="PKZ52_02365"
     CDS             2702..5158
                     /locus_tag="PKZ52_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNG58640.1"
                     /translation="MPFNTALSGIRAASNDLNITGNNIANASTTGFKSSRAEFGDVYA
                     SSILGSGSNPIGSGVRLQDVAQNFKDGNIAFTENELDLAVKGSGFFVVQKGGEQFYTR
                     AGLFSMDKDGYIVNNVNARLQGFDADDNGSIGGLQTDLRIQTSNLAPRPTTNVESNVN
                     LDSSEKVKQSVGKTLSTTGNAVGVTQGGLEQATSTTATGNAFTLGGIGNNFTTTPMTF
                     DVELTGAGANNGTVSINLSSARGVPATITSFNELRTLAGVINAQLSQPVPPQTAIDAT
                     AVAKDDGGGNYHLEFVSRTAGEGSQIRVLNSSANAAQLGLPAPAATLVSTAGIPKVTN
                     GYPKQSIELVDPKGKITTFQASSGATAADTAAKLGALTGVEATATTQATLSAANYVNS
                     TGSMVLTLNSVALTGDTLPAIADQINALTHGTLPGISAAVDAATGDLIITSSTGGDLH
                     FQISSPNDGDTVQIIGNSAAPPQILEVDNNATLNSPQALPASINSVVIGGTINIVLDE
                     GYSLQNANPPSVGLFGPLVTNAFTPITINAFDPVDVDTFNHSTTQTIFDSLGNAHQMQ
                     QYFVREKYDVNDPTTAPNHWKMYVLIDGKDVGDPDTTLPPPGNTLPTRASYDIFFNAD
                     GSINPSLTDDILISNWTPVDVNGNANGALGPQNVLAGGSVIIPDPPTSSNFVIDMGKS
                     TQFGSKFSVNHVKQNGYTTGRLSGLNIDAGGVIFARFTNGESQVLGQVALSNFSNPQG
                     LQPVGDTMWAENYESGAPNIGTPGSGALGAIQSGALEESNVDLSSELVNLIIAQRNFQ
                     ANSKTIETANQVTQTIINLR"
     gene            5372..6172
                     /gene="flgF"
                     /locus_tag="PKZ52_02370"
     CDS             5372..6172
                     /gene="flgF"
                     /locus_tag="PKZ52_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNG58641.1"
                     /translation="MNSNGVKRQINASEVVDKALYIAMTGAKHNMRGQTSHANNLANS
                     NTDGFKADFAQARSMPVYYGAGMPTRAYALAELGGTNFTSGTLNQTGRDLDVAIHGEG
                     FIAVQGRDGQEAYTRAGSFEIDSQGILRTAGGLMVLGNGGPITIPPAQKVEFGEDGTI
                     SVHAQGQTPSALVQVDRLKLVKPDLATVSKGPDGLFHVLSNPPGAVLPADASVKVIGG
                     SLENSNVNAVHELTGLLTLARQYEMHVKMMATVEKDSESAARLLQLNA"
     gene            6188..6973
                     /gene="flgG"
                     /locus_tag="PKZ52_02375"
     CDS             6188..6973
                     /gene="flgG"
                     /locus_tag="PKZ52_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNG58642.1"
                     /translation="MHAALYVSKTGLSAQDKQLSTIANNLANASTVGFKRDRAVFEDL
                     LYQIQRQPGAQTTQETQLPSGLQLGVGARVVGTQKQFTEGSLQVTEQSLDVAVDGRGF
                     LPIQQADGTIAYTRNGQLHLNREGQMVTADGLLLQPTIAVPNNANTVTIGTDGTVTAK
                     VVGEPEPQAIGNIQLVDFVNPAGLQAIGSNLFVQTGSSGEPIQGTPGENGLGQLKQGM
                     LENSNVNIVEEMVNMITTQRAYEMNSKVVSTADQMLQYITQNL"
     gene            6984..7661
                     /gene="flgH"
                     /locus_tag="PKZ52_02380"
     CDS             6984..7661
                     /gene="flgH"
                     /locus_tag="PKZ52_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNG58643.1"
                     /translation="MNKWHCICCILSALILSACTVSKPERPDDPYYAPKLVPTQGRAA
                     PQDGSLYTDAGLDLFSDRKARRPGDIITIVLKESTVSQKKSNLNSTKDTAASLDNPTL
                     LGSPLGLGKLDLTTGINAKRDFKGKGDAAQSNNLQGNISVTVSDILPNGVLVVRGEKW
                     LTLNRGDEYIRISGLVRPDDIGPDNTVLSTRVANARISYSGTGAMADSSQMGWLSRFF
                     NSGLWPF"
     gene            7686..8804
                     /locus_tag="PKZ52_02385"
     CDS             7686..8804
                     /locus_tag="PKZ52_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNG58644.1"
                     /translation="MMGHTHKTAQIILIVFATLCSLGAYAERIKDIASVQGVRSNQLV
                     GYGLVVGLDGTGDQTNQAPFTAQSFSAMLKQFGITVPEGAKFQLKNVAAVAVNAELPA
                     FAKPGQNIDVTVSSLANAKSLRGGTLLLTPLRAVDGNVYAIAQGNLVVGGFGVEGKDG
                     SKVTVNIPSAGRIPNGASVERTVPNQFAGGDNIVLNLHTADFTNAKRVAQSINDLVGP
                     NIAQAQDSVSISVQGPKNPNQRVEYLSLLENIEIEMADSAARIVINSRTGTIVVGKNV
                     RVSPVAVTHGSLTVTVKEDVQVSQPNALANGQTAVVPNSNVGVTQEKSPMFKFSPGPT
                     IEDIVKAVNQVGAAPGDLMAILEALKQSGALKAELVVI"
     gene            8805..9911
                     /gene="flgJ"
                     /locus_tag="PKZ52_02390"
     CDS             8805..9911
                     /gene="flgJ"
                     /locus_tag="PKZ52_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNG58645.1"
                     /translation="MATPNLPGAATSAGDIYTDVNSLAKIKNVKDPKAALSKVATQFE
                     SMFIGMMLKSMRQANAAFEQDGLFESKDSLFYRDMYDQQLSIELAKGRGMGIAKALER
                     QLSPMVSKAALPHSASAVAQDPNHLSASTSNEQTDNNDERRNLEWRRVIATSAPALIA
                     RNSSEAGEIEKNTPTYVEPLTHSDWLDPYLTPPVGAVQKPIDVNIKLTLNSPQDFIDQ
                     LLPYAKVAARDLGVNPLVLIAQAAMETGWGKRMVSDAAGNSSNNLFNIKANSAWNGDA
                     VEVATLEYKNGTPRKELAQFRQYSSIADSFKDYVNLLQNSPRYQAALAVANDAKQFLR
                     ELHGAGYATDPLYSQKIFSLFQTIADGRYKNDEE"
     gene            9915..13088
                     /gene="flgK"
                     /locus_tag="PKZ52_02395"
     CDS             9915..13088
                     /gene="flgK"
                     /locus_tag="PKZ52_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNG58646.1"
                     /translation="MASDLLGLSVSGLRASQTALNTTGHNIANANTEGYSRQRVEVAT
                     NPASYTGVGYIGNGVSVESIERVANQYVTAQVRTDAILFNEVDTFHKQVGALDNLLSN
                     EATGLSSALESFFNAMQNGSNDPTSIPARQLIISESANLADRFNSIYSRMDDIRSTVN
                     SAMDIVVGQVNALSSSIASLNLKITDARGVGSGNEPNDLLDQRDEALRKLSELVGIQV
                     FDQGNGQLNVLAGTGQSLVVGTRAQPLLIQADPSDAKQKQIFMQTELATMPVNVTGGQ
                     LGGLIKFRDEALNSGFNQLGRIALAMSTSLNTQHHQGITLDNQFGKDLFTDMNDPSLA
                     AGRVHSFATNTPPDDQQLGLYIEDMGKVGTSNYEVSIEGGGLFRIKRSSDGAEVASGL
                     MPGRFPFSAQFDGLKLQFNGGTFKDGDHFSLLPTQDAARVFKKTIVNPQELAFANPLS
                     TETALGNIGSGKISSGSVLSLVDKNSQPLKLFSEPGKMSPPLVVVFRSETTYDILDNT
                     DPGNPVQLDPPIRNQIYVPGSENKLFPEDKFVKTFTTQGDLLGLPEGRRPVVQAAVLP
                     AGIAPNFAQTDFSSPAAQFSFSVTVSETLNGSQDGVFNVRINQPAITDNTSLLQSINS
                     QLSSAGVRAFIADDGSLAFKLQNPGYGNITLGGYNADPDGAGGSATAGTANNLLGFNI
                     EAGSFTSVGNAQGTAGFGALNNGYPAEVVSISKASPIAGGTPTVYDVAIGLNASAKTI
                     ASSLNNIPGVSANAFTQATITGLNITHTEPLQLQLNGENLIPYVYDSLQSKFVIPDTV
                     PDPVANIDDFNDFIAAQINGNTNLQASGIFANAGVNATTGRPEIRVYAKFGDDLAVAF
                     TAAAGESIAVNDGENPDLTLTGQGNGLANAIAVGGQLDVAMNDDLTISTFADESLIFG
                     DTKVASYSQSNYLGIQASINGSPKTGDKFVLDFNHNAALDNRNALDLVNLQSKKTMAG
                     GLASFGEGYGAVVEAVGIQTNAAKINSEAAKQVLAQTTQLRDSLSAVNMDEEAANLVK
                     FQQLYSANARVIQTARELFNTLLQSFG"
BASE COUNT         3576 a         3040 c         3351 g         3396 t
ORIGIN      
        1 cctgcacaaa gccagatcta cgttctacgg taaccccagg gtagttagca acttttgcgt
       61 tggcgccagt taaacggtta aacagggatg ttttgccgca gttgggattg cccaccaacg
      121 catatcggct catggtgtac gcctaaatta aagagggata acaatcagct tatgggcttc
      181 tgcgcggcgc agggcgaatt tggtgccgtt gatctgtacc gccagagggt cgcggccaaa
      241 taagccatag cctataactg ttaactgcac cccaggcaaa aagcccaacg ccttcatgcg
      301 ggccgtaact gcgttgtcat ggtcgccaaa agcaggatta gggcgcagtt ctgtgagcat
      361 cacatgttgg cctttcgaac actctgtgag gctcaagcgg gaaacaggtg tggataggtc
      421 tggagatagg tattcggcag ttacggcttg cataggaata acctgcagat gaaattcatt
      481 atcatatgct atacgcacct agttgatccc acaagtgact tgagtcaaat cccgcagata
      541 tttaaagctt tgtctcccta agggctgcgt ttgcggcagc ttagggggcg actggtgtaa
      601 tcggcacgtc agagtctgtt aaaaacaata aattttctat ggcacagcca ttgctctagc
      661 ccctaataac tccttggggg acaaaattat ggcgatttct tttcgtaatt cagtgggtgt
      721 gtatgaatcc gctttaaagc tgcgcgctgc gcgcgccgaa atattggcgg gcaatctcgc
      781 caatgccgat acgcctggct accaggcaaa agattttaat tttgccgacg ccctgaaaaa
      841 tcagctggcc ggagcgccca atagcgaaga tttacggctt acgcaaccac tgcatcaaga
      901 agggctggcg ggcgcgggca gtggggggat ggatgtgcag tttcgcacgc ccacacagcc
      961 atcccttgat ggcaataccg tagaagagca ggtagaacac gctgaattta tgcagaacag
     1021 tttggaatac caaaccagtt ttacgctgtt aaatagccgt tttcgaggct taatgtctgc
     1081 cattaaaggt gaataattat gggcctaacc accatatttg atattgccgg ttccggcatg
     1141 aatgcgcaga gcctgcgctt aaataccacc gcgagcaata ttgccaatgc cgataccgcc
     1201 tcatccagcg cggataaaac ctaccggggg cgccatcctg tgtttgctgc cagttttccg
     1261 cagatgctgg ggcatcctga aaatgccaat gcaattgaca gcccgccgga gagccttggc
     1321 gttgccgtaa aaggaattgt ggagagcgat gcgcccttgc agccgcgcta tgaaccggaa
     1381 aaccctttag ccgatgaaca gggttatgtc tattacccaa acgttaatgt ggtggaagaa
     1441 atgaccaata tgatttccgc ctcacgctcg taccaggtga atgtagaggt agccaatgcg
     1501 gccaaacaaa tggtgcagaa aattttaacc ttgggccaat aattgcagta cctattacaa
     1561 catcttaaat aaacgcggaa atacaggttt ttaaacggat gttggctatc aagcggcaaa
     1621 ttatttccgc ccggatggca aaagcggcag ggattatcca gttgtgcggc aaatacagag
     1681 ctgtaaatcg agtgagtgaa taggtgtgtg ccaagtgaat agtatggggg ggaatcatta
     1741 tgagtgatat ttcaggctta gggtctgcca ttgatgcgct gtcgtcggcc aacaaatata
     1801 aaaaagatga aaaatcccct aatgagttgg ggcaggatgc ttttttacag ttgatgatat
     1861 ctcagctgaa aaaccaagat cctctcagcc cccaggataa tggcgagttt gtctcgcaac
     1921 tggcgcaatt tagctcggta gaagggctgg ataaactaaa taacaatttt gataccttcg
     1981 cgaataaatt tgtttccaac caagccttac aggcctccac cctggtgggg cgcacagtta
     2041 ctgtgccagg taaaaccact gcattagagc ccggcggcat agtgaccacc agtgctgaac
     2101 tgccagccgg ttccagtaat tttactttgg ctatttataa aaataatggc ggccttgcgc
     2161 aaacgattga ttttgacaag gtagatggcg atgaattaaa ggtcgcttgg aatgggcgcg
     2221 atgcggtagt taatggcaag gcagtcacta taccggagag ctttaaaggc gttgacccgg
     2281 gaatttatac cttcaaactc agcgctttgg tcgatggcaa gcgcacagag ttggataata
     2341 atgttacggc taatgttaat tccgtaaccc tgggtaaaaa tggcacctta acattaaatt
     2401 tggcgggtgc cgggtcggtt agtttatctg atgttaaaga aattaatgaa tagagcatgc
     2461 tgtggttaag cgtgaagttg agaatattgt gaaaatgaac atgtactgtc ccgtttgcgt
     2521 tatgttcaca ataatcctgt ttccacactt aattagacgt tctcagtatg ggtttttgcg
     2581 aggctgccgc gaggctgctt aaaaggccca gactgtaagc aagcaattta ataccagtgg
     2641 tgttgcttag ggaaaagtta cttattcaca gggccaattg atgtaaggct gggagacgta
     2701 tatgcctttt aatactgcac taagcggcat tcgcgccgct tcaaacgact taaatattac
     2761 tggcaacaat atcgccaatg cctccaccac ggggtttaag tcctcgcggg cagaatttgg
     2821 cgatgtctat gccagttcta tactgggctc tggcagcaac ccaattggta gtggtgttcg
     2881 tttgcaggat gtcgcgcaga attttaagga tggcaatatt gccttcaccg aaaatgagtt
     2941 ggatttggcg gtaaaaggct ctggtttttt tgtggtgcaa aaaggcggcg agcaatttta
     3001 tacgcgagca ggtttattta gtatggacaa ggatggctat atagttaata acgtaaatgc
     3061 gcggttgcag gggtttgatg ccgatgacaa cggcagtatt ggcggattgc aaaccgactt
     3121 gcgtattcaa accagcaatt tagcgcccag gccaacgacc aatgtggaat cgaatgtgaa
     3181 tttggattcc tcagaaaaag tgaaacaatc ggtaggtaaa acattgtcaa ccaccggcaa
     3241 tgccgtgggt gtaacccagg gtggtttaga gcaggcaacc agtacaacag caacgggcaa
     3301 tgcatttacc ctcggcggca taggtaataa ttttacaacc acacctatga cattcgatgt
     3361 ggaattaacc ggagccggag ctaataacgg caccgtctca attaatttat cttcggctcg
     3421 cggagtgccc gccaccatta ccagttttaa tgagttgcgc acattagcag gtgtgattaa
     3481 tgcgcaatta tcccagcctg tgccaccgca aacagcgatt gacgccacag cggtggctaa
     3541 ggacgatggc ggtggcaact atcatttgga attcgtatct cgcaccgccg gtgaggggtc
     3601 acaaataagg gtgcttaatt ccagcgctaa tgcggcccaa ttgggcctgc cagcaccagc
     3661 tgcgacttta gtgtcaactg ccggtatccc caaagtgact aacggttacc cgaaacaatc
     3721 tattgagctt gttgacccca aggggaaaat tactacattt caagcctcaa gtggcgcgac
     3781 tgcagcagat accgccgcaa aattgggtgc attaacggga gttgaagcta cagcaactac
     3841 gcaggctacc ttatcggcag cgaattacgt caattcaaca ggttctatgg tgctgacatt
     3901 aaattccgtg gcgctcacgg gggatacctt acctgctata gcggatcaaa ttaatgcatt
     3961 aacccatggc acattgccgg ggattagcgc tgcggtagac gcagccacag gtgatttaat
     4021 tattacctca agcactggcg gtgatttaca ttttcaaatt agcagcccta atgatggcga
     4081 cacagtgcaa attattggca attcagctgc gcctccgcaa atattggaag ttgataacaa
     4141 cgccacctta aattcacccc aggctttgcc cgccagtatc aactctgtgg tgattggtgg
     4201 caccattaat atagtgttgg atgaaggtta ttctttgcaa aatgccaatc cgccatctgt
     4261 tgggttgttc ggacctttag taaccaatgc gtttaccccg atcaccatta acgcatttga
     4321 tccagttgat gtggatacct ttaaccactc tactactcag acgatttttg atagcttggg
     4381 taatgcccac cagatgcagc aatattttgt gcgggaaaaa tacgacgtca atgaccccac
     4441 tacagccccc aatcattgga aaatgtatgt attaatcgac ggcaaagatg tgggcgaccc
     4501 ggacacaacc ttgccgcccc ccggcaatac tttgccgact cgcgcctcct acgacatatt
     4561 ttttaatgcg gacggctcca ttaatccctc tcttacagac gatattttaa tttccaactg
     4621 gacgcctgtg gatgttaacg gtaatgccaa cggcgccctc ggcccacaaa atgttttggc
     4681 gggcggcagt gtgataattc ctgacccacc gaccagctca aattttgtca ttgatatggg
     4741 taagtccacg cagtttggta gtaaattttc cgtcaaccat gtaaaacaaa acggttatac
     4801 cactgggcgt ttatccgggc tgaatattga tgctggcggc gttatttttg cccgtttcac
     4861 taatggcgaa tctcaggtgt tagggcaggt ggccttgtct aatttttcca atccgcaggg
     4921 attgcagccg gttggcgata ctatgtgggc cgagaattat gagtcgggcg cgccgaatat
     4981 aggcaccccc ggttccggcg cgctgggtgc tatacagtct ggtgctctag aggagtctaa
     5041 tgtggattta tcctcggaat tagtaaacct aattattgcc cagcgcaact tccaggcaaa
     5101 ctctaaaacc attgaaacgg ctaaccaggt gacgcaaacc attattaatc ttcgttaata
     5161 acagccacag gcgcaggcct gggccataaa taataagaat ccaaaaatta cttttcgaaa
     5221 agcccgcttg cgggcttttt tcatctcgcg catggggttg tcgcaaatgc aagcgtgcat
     5281 ccctgtagct ctgccgcaag cctcctgttt tattaacact caatttcctc cttcatatgc
     5341 gctgctatag gaatggcacc gctcttgcaa tttgaatagc aacggcgtta aacgccaaat
     5401 aaatgcgagt gaagttgtgg ataaggctct gtatatagcg atgactggcg caaaacataa
     5461 tatgcgcggg cagaccagtc atgccaataa tttggcgaat agtaataccg acggttttaa
     5521 agccgatttt gctcaggccc gcagtatgcc ggtttattac ggcgcaggta tgcctacgcg
     5581 ggcctatgca ttggcagaat taggcggtac caattttacc tcaggaacat taaatcaaac
     5641 cgggcgcgat ttggatgttg ctattcacgg cgaaggcttt attgccgtgc agggccgcga
     5701 tggacaagag gcatatacac gggcgggcag ttttgaaatt gatagccaag gtattttacg
     5761 cacagccggt ggtttaatgg tgctgggcaa tggtgggccg attacgattc ctccagcgca
     5821 aaaagtagaa ttcggtgaag acggtactat cagcgttcac gcacaaggcc aaacaccctc
     5881 cgcattagta caggtggatc gccttaaatt agtcaaaccg gatttggcta ctgtgtcaaa
     5941 aggccctgac ggtttattcc atgttttatc caatccgccc ggtgcggtat tgccagcgga
     6001 tgccagtgta aaagtaatag gcggtagttt ggaaaatagc aatgtcaatg ccgtacatga
     6061 attaacggga ttgttgacgc tcgcgcgcca gtacgagatg cacgtaaaaa tgatggcaac
     6121 ggtggaaaaa gattctgaaa gtgcagcgcg tttgctgcag ttaaacgctt aattatcgag
     6181 gcattttatg cacgcggcac tttatgttag taaaaccggt ttatcggccc aggataaaca
     6241 actctcaacc atcgccaata accttgcaaa cgcatcgacc gttggtttta aacgcgatag
     6301 agctgtattt gaagatttgc tctatcaaat tcagcgtcaa cccggcgcgc aaaccaccca
     6361 agaaactcag ttgccttccg gtttgcaatt aggcgttggt gcccgtgttg tcggaacaca
     6421 aaaacaattt actgaaggta gtttgcaggt aacagaacag tcattagacg tggcggtgga
     6481 tgggcgcgga tttttaccta ttcaacaggc ggatggcacc attgcataca cgcgcaatgg
     6541 gcaattacat ctcaaccgcg aaggccaaat ggtgacggca gatgggctgc tgctgcaacc
     6601 cacaattgcc gttcccaata atgctaatac cgtcaccatc ggcacagacg gaactgtaac
     6661 tgcaaaagtg gtgggtgagc ctgagcccca agccattggt aatattcaat tagtggattt
     6721 tgtgaatccg gcaggtttac aagctatagg cagtaattta ttcgtgcaaa ccggctccag
     6781 tggcgagcct atacaaggca cccccggtga aaatggtttg gggcaattaa aacagggaat
     6841 gctggaaaat tctaacgtga atattgtaga agaaatggtc aatatgatta ccacccagcg
     6901 cgcctatgag atgaattcta aagtcgtctc tacggcagat caaatgctgc aatatattac
     6961 gcaaaatctt tagtgagttt tttatgaata agtggcattg catctgctgc attttaagcg
     7021 cgctgatatt atccgcctgc acggtaagta aacccgagcg gccggatgac ccttattatg
     7081 cgccgaagct ggtgcctacc caagggcgcg cggcacccca ggatggcagc ttgtacaccg
     7141 atgcggggtt ggatttattt tccgatcgca aagctcgccg cccaggggac attattacga
     7201 ttgtgctaaa agaatccaca gtttcacaaa agaagtcgaa tttaaattcc actaaagata
     7261 cggcagcatc cttggataac ccgactttat taggttcccc cctggggctt ggaaaattag
     7321 atttaactac aggtattaat gctaaacggg attttaaagg caagggtgat gctgcgcaaa
     7381 gcaataattt gcaaggcaat atttcggtga cggtgagcga tattttgccc aatggtgtgt
     7441 tggttgtgcg cggagaaaaa tggctaaccc tgaaccgggg tgatgaatat atccgcatta
     7501 gcggcctggt gcggcctgac gatattggcc ctgataacac agttttatcc acccgtgttg
     7561 ccaatgcgcg tattagttat tccggcactg gcgcaatggc ggatagtagc caaatgggct
     7621 ggcttagccg atttttcaac agcgggctgt ggccatttta acggcggcga ttgcaagagg
     7681 tatttatgat gggacataca cacaaaaccg cgcagattat tctgattgtt ttcgccacac
     7741 tgtgtagctt aggcgcttat gccgagcgga taaaagatat agcctcggtg caaggggtgc
     7801 ggtccaatca gttggtgggc tacggcttag tggtgggcct ggatggtacg ggcgaccaaa
     7861 caaaccaggc accttttacg gcgcaatctt tttccgccat gttaaaacaa tttggtatta
     7921 ccgtgccgga aggcgccaag tttcaattaa aaaatgtcgc ggcggtggcg gtgaatgcgg
     7981 aattacccgc ttttgctaaa ccggggcaaa atattgatgt gacggtatca tcccttgcca
     8041 acgcaaaaag tttgcgcggc ggcaccttgt tattaacacc gttgcgcgca gttgatggca
     8101 atgtatatgc aattgcgcag ggcaatttag tggtgggggg atttggtgtt gaaggcaaag
     8161 acggctccaa agttacggta aatattccca gcgctgggcg cattcccaac ggcgcttcag
     8221 ttgagcgcac cgtgcccaat caattcgctg gtggcgacaa tattgttttg aatttacaca
     8281 ccgctgattt taccaatgct aaacgggtgg cacagagtat taatgattta gtcggcccca
     8341 atatcgcgca ggcacaggat tcagtatcca tcagtgtaca gggccctaaa aatcccaatc
     8401 aacgggttga atatctgtcg ctattggaaa acatcgaaat agagatggcc gatagtgccg
     8461 cgcgcatcgt aattaattcc cgcacgggca ccatagtggt gggtaaaaat gtgcgtgttt
     8521 cgccggttgc cgttacacac ggctcactca cggtgacggt aaaagaagat gtacaggtga
     8581 gccagcccaa tgctttggcc aatggccaaa ccgcagtggt gcccaatagc aatgtaggcg
     8641 ttacccagga aaaatcgccc atgtttaaat tctcccctgg gccaacaatt gaggacatag
     8701 taaaagcggt gaatcaagtg ggcgcagcgc ccggtgattt aatggctatt ttagaggcgc
     8761 taaaacaaag tggcgcatta aaagcagaat tggtagtgat ttaaatggcc acgcctaatt
     8821 tgccaggtgc agcaacttct gctggggata tttataccga tgtaaactcc ttggcaaaga
     8881 taaaaaatgt caaggacccc aaagcagcct taagcaaggt ggctacacag tttgaatcca
     8941 tgttcatcgg catgatgctt aaatccatgc ggcaggccaa tgcggcattt gagcaggatg
     9001 gattatttga atccaaagat tcgctgttct accgcgatat gtacgatcaa caattatcca
     9061 ttgagctggc caaagggcgc ggtatgggca tcgctaaagc attggagcgc cagttatcgc
     9121 ccatggtatc gaaagcagcc ttgcctcatt cagcttctgc tgtagcacaa gatcccaacc
     9181 atctttcggc cagtacgagt aacgaacaga ctgataataa tgatgagcga cgcaatctgg
     9241 aatggcgccg agttattgcg accagcgctc ctgctttaat tgcgcgtaat tccagcgaag
     9301 cgggtgaaat agaaaaaaat acaccgactt atgtagagcc gcttacgcac agcgattggt
     9361 tagatcctta tttgactcca ccagttggcg ccgtacaaaa gcccattgac gttaatatca
     9421 aattaacgct taacagtccc caggatttta tcgaccaatt attgccctat gccaaagtgg
     9481 cagcccgtga cctaggtgtt aatccgctgg tgttaattgc ccaagcagcc atggagaccg
     9541 ggtggggaaa acgcatggtg agcgatgctg ccggtaactc cagcaataat ttatttaata
     9601 taaaagccaa ttcggcctgg aatggcgatg cggttgaagt ggcgaccttg gaatataaaa
     9661 atggtacacc gcgcaaagaa ttggcgcaat ttaggcaata ttcctccatc gctgatagtt
     9721 ttaaggatta tgtcaacctg ctgcaaaaca gcccccgcta tcaagccgca ctagcagttg
     9781 ccaacgacgc caaacaattt ttacgggagc tacatggcgc aggttatgca acagatccac
     9841 tatacagtca aaaaatcttt agtttatttc agaccatagc ggatggccgc tataagaatg
     9901 atgaagaata acctatggct agcgatcttc tcggcttaag tgtaagtggt ttgcgcgcca
     9961 gccaaacggc gcttaatacc actgggcaca atattgccaa tgccaacacg gaaggttact
    10021 cccgccagcg agtggaggtt gccactaatc ctgccagcta tacaggcgtg ggttacattg
    10081 gtaatggcgt ttcggtggaa tccattgagc gtgtggcgaa tcaatatgtc accgctcaag
    10141 tgcgcacaga cgccatttta tttaatgaag tggatacttt tcataaacag gtgggcgcct
    10201 tggataattt attgtccaat gaggcaaccg gtttatcctc cgcgttagaa agttttttca
    10261 acgccatgca aaatggcagc aatgacccca cctctattcc tgcacggcaa ttaattatca
    10321 gcgaatctgc caacctggcc gatcgtttta atagcattta ttcgcgcatg gatgatatcc
    10381 gtagcacggt taattccgcc atggatatag tagtggggca ggtaaatgcc ttgtcatcga
    10441 gtatcgcttc cctcaattta aaaattaccg atgcccgcgg tgtgggttcc ggcaatgagc
    10501 cgaatgattt gttggatcag cgggatgaag cgctgcgcaa attatcggaa cttgtaggta
    10561 ttcaggtatt tgatcaaggc aacggccaat taaatgtctt ggcgggcaca ggccaaagct
    10621 tggtggtggg cacccgcgcg caaccgctat taattcaggc agacccaagc gatgccaaac
    10681 aaaaacaaat ttttatgcaa accgaattgg cgaccatgcc ggttaatgtt accggcgggc
    10741 agttgggcgg cttaattaaa tttcgcgatg aagcccttaa ttcaggtttt aatcagctag
    10801 ggcgcattgc tttggctatg agcactagct taaataccca gcatcaccaa ggcatcacct
    10861 tggataatca attcggcaaa gacttattta ccgatatgaa tgatccgagt ttggctgcag
    10921 gccgagtaca ttcgtttgct actaatactc cgccggatga ccagcagttg ggcttgtata
    10981 tcgaggatat gggcaaagtg ggcaccagca attacgaggt gtctattgag ggcggtggtt
    11041 tatttcgcat taagcgcagc agcgatggag cggaggttgc cagcgggttg atgccggggc
    11101 gctttccgtt tagtgcgcag ttcgacggat taaaattaca atttaatggt ggcaccttta
    11161 aagatgggga tcatttttca cttctgccca ctcaggacgc cgcacgggtt tttaaaaaaa
    11221 ccatcgtaaa cccccaggag cttgcctttg ccaatccact ttcaaccgag acggcgctgg
    11281 gtaacatagg cagtggcaaa atcagttctg gctcagtttt atccttggtg gataaaaaca
    11341 gtcaaccgtt aaaattattt tcagaacctg gcaaaatgag cccgcctttg gtagtggtgt
    11401 ttcgatcgga aactacctac gatattttag acaatacaga tcccggcaat ccggtgcagt
    11461 tagacccgcc tattcgcaat caaatttatg tgccgggcag tgagaataaa cttttccctg
    11521 aggataagtt tgttaaaaca tttactaccc agggtgattt actcggcttg ccggagggca
    11581 ggcggcctgt ggtacaagcc gccgtattgc cagccggtat cgcgccaaat tttgcgcaaa
    11641 cagatttctc atcacccgcc gcacagtttt cgttttcagt tactgtttca gaaaccctaa
    11701 acggtagcca ggatggtgta tttaatgtgc ggattaacca gcctgctatt acagataata
    11761 caagcttatt gcagtctatt aatagccagc tatcgagcgc gggtgtgcgt gcatttattg
    11821 ccgatgacgg cagcctcgca tttaaattgc aaaatcctgg ttacggtaat atcaccctgg
    11881 gcggttataa tgccgaccct gatggtgcgg gtggcagtgc taccgcaggc actgctaata
    11941 atctgttggg ttttaatata gaagcgggca gttttaccag tgtgggcaat gcccagggta
    12001 cagcgggttt tggagcgtta aataatggct atcccgccga agtagtttct atctctaaag
    12061 cttcgccaat tgctgggggt acacctacag tttacgatgt ggcgattggc ttaaatgcat
    12121 cggcaaaaac aattgcatcc tcactcaata atatacccgg tgtttcagcc aatgctttta
    12181 cccaggcgac tattacaggc ctaaatatta cccatactga gccgctgcag ttgcagctca
    12241 atggtgaaaa cctcatccct tatgtctatg attcgctaca aagtaaattt gtgattcccg
    12301 atacggtgcc agacccagtt gcgaatattg acgattttaa tgattttatc gctgcgcaaa
    12361 ttaatggcaa caccaattta caagccagcg gcatttttgc taatgcgggt gtgaacgcta
    12421 ccaccgggcg gccggaaatt cgcgtctacg ctaaatttgg cgatgattta gctgtggcgt
    12481 ttacagcggc agcaggtgaa tccattgcgg ttaatgacgg cgaaaaccct gatcttaccc
    12541 ttacagggca gggcaatggt ttagcgaatg ccatagcggt gggcgggcag ctggatgttg
    12601 ccatgaacga tgatctaact attagcacct ttgccgatga gagtttaatt tttggcgaca
    12661 ctaaagtggc aagctatagc cagagcaatt atttaggtat acaagcgagt attaatggct
    12721 cgcctaaaac cggcgataag tttgtgcttg attttaatca taacgctgca ttggataacc
    12781 gcaatgccct tgatttagta aatttacagt ctaaaaaaac catggcaggt ggccttgcca
    12841 gtttcggtga aggttatggc gctgtggttg aggcggtggg cattcaaacc aatgctgcaa
    12901 agattaattc agaagcggct aaacaagtcc ttgcccaaac cacgcaattg cgggactcgc
    12961 tctcagcggt aaatatggat gaagaagcgg ccaaccttgt aaaatttcag cagttgtatt
    13021 cggccaatgc ccgcgtgatt caaaccgcac gagaattatt caatacgcta ttgcagtcat
    13081 tcggttaaat ctaaaaacag cagccgggtt taaatagtgt ttttaggtta gatagcgctg
    13141 agttaatttc gctgtaactg tttggtacac ttctttagca cacgttattc cccacttccg
    13201 cgcgggcggc caatgctgga ggatttagtt atacggcaca atcaatttgc ggtggagcag
    13261 cggtgactag gggttagtac aaaccgccaa aaaggtcttt cttattagcc cggcggctaa
    13321 atagatcgtc ctgatctatt tagccttcgc tgccctaaaa ctg
//
LOCUS       DAOQGS010000036        13262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2173_length_13262_cov_4.214280, whole genome shotgun sequence.
ACCESSION   DAOQGS010000036 DAOQGS010000000
VERSION     DAOQGS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2173_length_13262_cov_4.214280"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKZ52_02400"
     CDS             complement(<1..255)
                     /locus_tag="PKZ52_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58647.1"
                     /translation="MKIWIQACAVLILASQMTACGGGDSSAAPSSNTEVNTPVQAAPT
                     AAESKALADTYNAAQTLWANTAPTNYHYSYKEGGQGYKFDA"
     gene            933..1107
                     /gene="ssrS"
                     /locus_tag="PKZ52_02405"
     ncRNA           933..1107
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKZ52_02405"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1145..1759
                     /locus_tag="PKZ52_02410"
     CDS             1145..1759
                     /locus_tag="PKZ52_02410"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNG58648.1"
                     /translation="MQNPCTENSPCKTSLRRQLRTYRRALSPAHQRRAALRLARNFLH
                     LPALKQARSFALYIASDGEIDPSVLLKRLTAMGKQIYLPRLNRAATAMQFVRFKAGEP
                     LQRSRLGVLQPAPGAGAIALHRLDIVALPLVGFDGLGNRLGMGGGFYDRALQARGRRP
                     LLVGLAHAGQQLARLPVEPWDVPLDWIATDRRLLRCPPVRRLMG"
     gene            1822..2580
                     /gene="cysZ"
                     /locus_tag="PKZ52_02415"
     CDS             1822..2580
                     /gene="cysZ"
                     /locus_tag="PKZ52_02415"
                     /inference="COORDINATES: protein motif:HMM:NF003433.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HNG58649.1"
                     /translation="MQNLLRHSGPGYFLLGLKLIWQPPLRAFVIWPVLLNIALFAASI
                     GLLLHGLGQWLDGFLSGLPAWLDFLRWLLWPLIALCVLAAYGYGFNLITTLIGAPFYG
                     LLAQKVQVLLGHPAPPDEPFAQLLRRTFAREWQKLCYFVPRSLILVALSLLALLIPGL
                     NLVMPLLFFIWGAWCLAIQYCDYAADNQLQSFAGLKQQLGQHKALSLSFGGSCAGASM
                     LPVFNILAIPAAVAGGTALWLGRDKLSVPAGGLL"
     gene            2577..3434
                     /locus_tag="PKZ52_02420"
     CDS             2577..3434
                     /locus_tag="PKZ52_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013203448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HNG58650.1"
                     /translation="MKVPARLQPLVADGIIDEVLYPLKSGKEAQIFVVRADGEIVCAK
                     VYKEASQRSFKQAAQYQEGRTYKGGRRSRAMAKNTRFGQAEAEKAWLNAEVEALKCLA
                     AAGVRVPTPYSYVDGVLLMELIQDESGDPAPRLDDVAFPEDIAADYHRHMMGEIAKML
                     CAGLVHGDLSEFNVLVDAYGPVIIDLPQAVDAARNNNAKAMFMRDINNMTQYFGRYNP
                     SLLSTRFGEEIWQLYQHGELTPASELTGVFTEVLTEVDLDAVLSVIEHAKAEEAERQA
                     RLLADQNDD"
     gene            3450..4820
                     /locus_tag="PKZ52_02425"
     CDS             3450..4820
                     /locus_tag="PKZ52_02425"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNG58651.1"
                     /translation="MTDTPRFYWGTATAAYQIEGAWLAGGKGLSIWDAFAHTPGKVEA
                     GHTGDTACDHYHRWAEDIALMQQLGVNAYRFSIAWPRLFPQGRGSQNPEGVAFYNQLI
                     DGLVAAGITPFVTLYHWDLPLTLQLEQDGWLNRQTAEDFAAFAQFCFGAFGDRVRHWI
                     TFNESWCTAVLGYGIGYFAPGRKSPDEPYVAGHNLLIAHGLAVEAFRTGGFAGQIGLA
                     SNCDWREPLSDSPEDKAAAQRSLEFFYGWFTDPVVFGDYPAVMRERLGARLPQFSPAD
                     QARLKGSVDFLGLNHYTTHLASALPPSAHAIAPEAGNGGMAADQGVYLSCDPTWPRTD
                     MGWFVVPWGFRKMLNWVHQRYPNLPIYVTENGCANPHPEEETSQADRFRVDFLQGYLG
                     ALEQARKQDGVPVQGYFCWSLLDNFEWAYGYSKRFGLIRCDFASGARTPKNSFYFYQH
                     YLRQHL"
     gene            4817..5170
                     /locus_tag="PKZ52_02430"
     CDS             4817..5170
                     /locus_tag="PKZ52_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNG58652.1"
                     /translation="MSQAADTPSPCMSCGACCAYFRVSFYWGEQQSAGGLVPDELTQK
                     ISPHLSCMQGTGSKPARCVALTGDIGQSVACSIYAQRSTTCREFAMSGENGEHNPECD
                     KARAFHGLAPLVWRV"
     gene            complement(5470..6789)
                     /locus_tag="PKZ52_02435"
     CDS             complement(5470..6789)
                     /locus_tag="PKZ52_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNG58653.1"
                     /translation="MTATPLLSAQSLSAQLKATKQRKGEIARQFKTLTAGTPEHHAAL
                     AAMQAITAELKALEADITALSAPQAPPEPQAKPQPLPALTLIDNTASHLAPFHCRALA
                     PQEWGLWDAYVAKVCSANNYHYAIWLQLIKEQFGHEGIIWVAFDEQGRIIGGIPLVIF
                     ASQLFGRFAVSVPYFNYGGVLSEFFNVAQELIATAAVLCEQLNLRHIEVRCMQQGLMP
                     NGHCSSKKASLILPLPTNQSQLEQQLGAKVRAQYKKALEHTPQFTIGKSELLAEFYSV
                     FAQNMRDLGTPVYAKSWFARLLEEPSLHCHIALVHMYGKPVSAGFLIGHGNMLEIPWA
                     STVRKANGWNANMWMYRQILGFAIEQGYQFFDFGRSTQDAGTFKFKRQWGAEPYSHYW
                     YYLLPPGGAMPGLNPDNPKYKLIINIWKKLPVWLTKIIGPPIVANLP"
     gene            complement(6871..7872)
                     /locus_tag="PKZ52_02440"
     CDS             complement(6871..7872)
                     /locus_tag="PKZ52_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNG58654.1"
                     /translation="MSNLADYIYTDPRIERLLTGLLGSWATIFTLHRPPAASGAYEGI
                     SPEFLAQALSFVKARGYEFVSVDELVARALRGDSLRRCLCFTLDDGFADQLEQLVPVL
                     LRYEAKPTLFVITDLIDGAAWPWDNQLAHLCWHAPAGLYSLELSGQAVAVDLSSPEAR
                     RKTRRQLTRLAKALQRPAIEALLGQLQACVGFSIPQAAPPEYQAATWEQLRWAEAAGL
                     RIGCHARSHFTFNALSDGEVAAELAHAKARLCAELVQPSEVFCYPSGTQNDFASHHEP
                     LVQQAGFLAAVSTLSKNTCAQAIQQAPYRIQRIGMPQQLNHFVRYISWFEYLRGRMA"
     gene            complement(7883..8881)
                     /locus_tag="PKZ52_02445"
     CDS             complement(7883..8881)
                     /locus_tag="PKZ52_02445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNG58655.1"
                     /translation="MLKPFIKSLAQQAAFAAGPQRWHTGAPQLWVLMYHRILPADDAR
                     CKAEEPGMCVTPEVFAMHLAQLRRYFTPMFLSDWVAKSRRGEALPARACAVTFDDGWR
                     DNFEFALPLLEQARVPATLFAVAEKIGTDFQFWPNLVANLLLQGALPQLQAHPIFQAL
                     HEAVPGLATGVNRERLALAIHFLKRYPDQEIFTALAQLNGQSLAAPLPRALMDWHELA
                     QMQASGWVEIGSHTCTHRRLTSALDESQLRYEIQHSQSLLQERLGRAVNLFCFPNGDY
                     SPTALQIVEETYQAAVTTQRGIAHVGQNLHELKRIGLHNDISATPVAFGARLSAWM"
     gene            complement(9030..11639)
                     /locus_tag="PKZ52_02450"
     CDS             complement(9030..11639)
                     /locus_tag="PKZ52_02450"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG58656.1"
                     /translation="MNGLMRALLVITPLLCLSACDRSAKQKVDESQRHLNTARAYQQQ
                     GQWRAAMIEARNAASKAEQPVEGLALLADIYMQIGANQAAAELLEKIARDDLALNIQW
                     AKALIAERKYQSAQKVLLGLPAAVRNNSAEFHQLMVQVGALEGDFALAQEHLSQLEQT
                     ADSPAAVAFWRAQLMAAQGDWSGAQALLKQWVEQKPQDQEAWVLLGQIALTANQLEEA
                     EAYLSKALALVPTGDMLSAAKARVLNLLTEVLTRLGRSSEAYHYQKMLAEANPEGAAA
                     QQKFNQALQLYQKGDFDQAQAVISELKKQFPQDKNTNTLLGLVQLQQGDTKQAQAIFD
                     ATIDPETAAPRVLQAAALSRIRNQQIDEALELLKKAAQNQPKSAEILATYGIALLDKD
                     PANAEAIEWLDKSLQLQQNQPKIYMAKARYFAGQNKMDEAVAQLRTALAQDPQHFETQ
                     QILFQLLFANKQLDFITQIINSLPESLAGRKYFWQGWLALQQKNYPQALSAFEQAVPL
                     SGAEKYLPLAGSAQVFQAQADWARAFPAWQQVIAAAPGFIPAYEHYLAAAKQVHQLEQ
                     AEVFLAAQSRTDENWQANWVLAQWFAANNDIDKAIVNAEEVHRKAMQLPSIKQLLVQL
                     YTQKAQLLKSSDILHSRDWINKALKLQPENPAILTFLVELELADEKIAQARAIIAEYA
                     KGNAEPALLNFLNGLVLLAEGKKAEALALLQVSFTAQPMDKSGELIYAILNSNDAAKA
                     VEFLAQWRKALPQSARPLLIQALAAQGAGDKAAAISAYEQALQLQAPSVLALNNLAWL
                     YFETKDARAIETAKRAVALAPNAAEVLDTYGWILVQTGNKAEGIRWLEKAKTLAPNLP
                     EILKHLEEAKGGK"
     gene            complement(11663..12952)
                     /locus_tag="PKZ52_02455"
     CDS             complement(11663..12952)
                     /locus_tag="PKZ52_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58657.1"
                     /translation="MVYQRSITLSLLCSACVSAWGAPAQDHFSADLSMESIFTDNALK
                     TSGDGLNERQDTLSVGLSGQQINRWSSFKLGYSADRTYFSQASQEDANTLEGDAQWVL
                     GKEYDWFNLTLQDSRRKVLRSNVATPLLSNTGERELLTLKPQLRLHVSPVDQIILGVS
                     REAVRLKRLAAADANRDTADLVWQHSLSATDQLLVSINQGESTRKDAEEADYKYQIAR
                     LGYQAQLSHLAYGIQLGYQNTKARGQEEFGKPNYNLDISYDKNGHNLSIGASSAVTDT
                     LFGDGNGASLDETLMQDSLSQNYDLVKLSQANLSWTGPKLWRGLRLSAQAQYSNHDYY
                     VLPEDYTDRAVSLSINYSFAARTHGNLRLEHSESRFDQVGKLSDYQEDNVLLRISHDF
                     TKALSADFLLGTIKRHSDDALSEYDELRTGVSVRYQF"
     gene            complement(12963..>13262)
                     /locus_tag="PKZ52_02460"
     CDS             complement(12963..>13262)
                     /locus_tag="PKZ52_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNG58658.1"
                     /translation="SGIERLRVIPSGGSSESAAEFFNSKRMEVLVAEIKERYSDRFIV
                     LDAPAIQQSTEARILAQYCDFSMLVVPFGKVVTDEVLSAIDALGKEHFAGLVFNS"
BASE COUNT         2918 a         3737 c         3591 g         3016 t
ORIGIN      
        1 ggcgtcgaat ttgtagccct ggccgccttc tttgtagctg tagtggtagt tggtgggggc
       61 ggtgttggcc cagagggttt gggcggcgtt gtaggtgtcg gccagggcct tagactctgc
      121 cgcagtgggg gcagcttgca cgggggtgtt cacctcggta ttgctgctag gggcggcgct
      181 ggagtcgccc ccgccgcagg cggtcatttg actggcgagg atcaacactg cacaggcttg
      241 tatccagatt ttcataaccg ctgcaccttc aacatctcta aaaaaatcgc cctgctgtaa
      301 caggtggtca cattttaaac agaagagccg aaagaaaccg gactttattg gcaatttaat
      361 caccaattag actaataaat aatcttttgc gggttttagt tatatcaacc ccctgctaac
      421 caatgtcatt gcgttaaggc gcaatagtag gggcggccct gtggttgggc tgcgtgagcc
      481 tgggctatgg gccaggattt atctgggaac gggcacccac aaggggtgcc cctacagctg
      541 tgtgtgcgct gagaaatcct tacctcaaac tgccctgcgg gataaggctt gggccataga
      601 ggcccttaag ggtttaactt aaggccctag ggtaaatact acaggcaaaa aaaagcccct
      661 atgcagagca gaggggcaaa gaccattagg agtgaaacaa tgaggaaaac ctcggctgcc
      721 acctgcgtca aacaggtttc agtactttga gtattgcagc ggatggcgga atgtctagcc
      781 gagggttaca tcaaatagca gcaacagatt gaaaacccta acgtttttgt tctagtactg
      841 atagcccata aacagcgtaa agggggaatt atatttttta gctacatggg gccgaaaagc
      901 ttggcggcgg cgtcagcttt gttatacttc aatcactcct ggggtgttcg ccagccgact
      961 aatccctgag ccgataaatt tacttggagt tctcgcgcat taccctgtga gcatgtccac
     1021 tcgatggaaa gcctaatcgg taacagagtc ctcccccttg aactgtcggg ttcaaggtat
     1081 agttgtcagc ggcatcccag gctttatttc ccgccttctc tatcccttct agtgtttcgc
     1141 cccagtgcaa aacccttgta ccgaaaacag cccctgtaaa acatccttaa gacgccagtt
     1201 gcgcacctat cgccgcgctc tttcgcccgc ccatcagcgc cgcgccgcct taaggcttgc
     1261 gaggaatttt ctgcacctgc ccgcccttaa gcaggcccgc agcttcgcct tgtatatcgc
     1321 cagcgacggc gagatagacc ccagtgtgtt gctcaaacgc ctaaccgcca tgggtaaaca
     1381 gatttatttg ccgcgcctta accgggccgc cacggccatg cagtttgtgc gctttaaagc
     1441 gggcgagccg ttgcagcgca gccgtttggg tgtgctgcag cccgcccccg gcgctggcgc
     1501 tattgcgctg caccggttag atattgtcgc cctgcccctg gtggggtttg acggcttagg
     1561 taaccgctta ggcatgggcg gcggttttta cgaccgggcg ctgcaggcgc ggggccgccg
     1621 cccgcttttg gtgggccttg cccatgcggg ccaacagctg gctcggctgc cggtagagcc
     1681 ctgggatgtg cccctggatt ggattgccac cgaccggcgc ctgctgcgct gcccgccggt
     1741 tcgcagattg atgggataac ggtaggcacc gcgagcggca gtttgtatag tggcggcagt
     1801 tttagttacc gcgtggagtt tatgcagaat ttgttgcgcc attctgggcc tggatatttt
     1861 cttctggggc ttaaactcat ctggcaaccc ccattacgcg catttgtcat ttggccggtg
     1921 ctgctgaata tcgccctctt tgccgccagc atcggcctgc tgttacacgg cctcgggcaa
     1981 tggttggatg gctttttaag cggcctgccc gcctggctgg actttctgcg ctggctgctc
     2041 tggcccttaa ttgccctgtg tgtactggcc gcctacggct atgggtttaa cctcatcacc
     2101 acgctcatcg gagcgccctt ttacggcctg ctggcgcaaa aggtacaggt gttattgggc
     2161 cacccggcgc cgccggatga acccttcgcg caactgctca ggcgcacctt cgcgcgggaa
     2221 tggcagaagc tgtgttactt cgtgccgcgc agcctcatct tagtcgccct atctctgctg
     2281 gcgctgctga ttcccggctt aaacctagtt atgcccctgc tgttttttat ctggggcgcc
     2341 tggtgtttgg ccattcaata ctgcgattac gccgccgaca accagctgca gagctttgcc
     2401 ggccttaaac agcagctggg ccagcacaag gccctcagcc tgagttttgg cggcagctgc
     2461 gcaggcgcca gtatgctgcc ggtttttaat atcctcgcca ttccagccgc cgtggccggt
     2521 ggcaccgccc tttggttggg gcgcgataaa ctcagtgttc cggcaggagg cctgctatga
     2581 aagtacccgc ccgcttacag ccgctggtgg ctgacggcat tattgatgag gtgttatacc
     2641 cattaaaatc cggcaaagaa gcgcagattt ttgtggtgcg cgccgacgga gagatcgtct
     2701 gcgccaaggt gtacaaagag gcttcccagc gcagttttaa acaggccgcc cagtaccaag
     2761 agggccgcac ctacaagggc ggccgccgca gccgcgccat ggcgaaaaac acccgcttcg
     2821 gccaggcgga ggcggaaaag gcctggttaa acgccgaggt ggaagccctt aagtgcttag
     2881 cggcggccgg tgtgcgggtg ccaaccccct acagctatgt ggatggggtg ctgctgatgg
     2941 agctgatcca agatgagagc ggcgacccag cgccgcgctt ggacgatgtg gccttccccg
     3001 aggacatagc cgccgattat caccgccata tgatgggcga aattgccaaa atgctctgtg
     3061 caggcttggt ccatggcgac ctgtccgaat ttaacgtgct ggtagatgcc tatggaccgg
     3121 tgattatcga cctgccccag gcggtggatg ccgcccgcaa taacaacgca aaagccatgt
     3181 ttatgcgcga catcaacaac atgactcagt acttcggccg ctataacccc agcctgctca
     3241 gcacgcgctt tggtgaggag atctggcagc tgtaccagca cggcgaactc acccccgcaa
     3301 gcgagcttac cggcgtcttc acagaggtgt taaccgaagt ggatttagat gcggtgctga
     3361 gcgttatcga acacgccaag gccgaagagg ccgagcgcca ggcgcgcctg ctggccgatc
     3421 aaaacgacga ctaacccgcg cccgccgcca tgaccgacac accgcgcttt tactggggaa
     3481 ccgccaccgc cgcctatcag attgaaggcg cctggttggc aggcggcaag ggcctgagca
     3541 tttgggacgc cttcgcccat acccccggca aggtggaggc gggccacacc ggcgacaccg
     3601 cctgcgacca ctaccaccgc tgggccgaag acatagccct gatgcagcaa ttgggcgtta
     3661 acgcctaccg cttttccatc gcctggccgc gcctgttccc ccaggggcga ggcagccaga
     3721 acccagaggg cgtcgccttc tacaaccaac ttattgacgg cctggtggcg gcgggtatta
     3781 ccccctttgt caccctctac cactgggatt tacccctaac cctgcagctg gagcaggacg
     3841 gctggctgaa ccggcaaacc gccgaagact tcgcagcctt cgcgcagttt tgttttggcg
     3901 cctttggcga ccgggtgcgc cactggatca cctttaacga aagctggtgc acggcggttt
     3961 taggctatgg cataggttac ttcgcccccg gccgtaaaag cccggatgag ccctatgtgg
     4021 cggggcacaa tttgttaatt gcccatggtt tggcggtgga ggcgtttcgc acagggggct
     4081 ttgcggggca aataggcctg gcgagcaact gcgactggcg ggaacccttg agcgacagtc
     4141 cagaggataa ggccgccgcc cagcgcagcc tggaattttt ttacggctgg tttaccgacc
     4201 cagtagtgtt tggcgattac cccgccgtga tgcgtgagcg cttaggcgcg cggctgccgc
     4261 agtttagccc tgcggatcag gcgcggctaa agggctcggt ggattttctc ggcctcaacc
     4321 actacaccac ccatttagcc tccgccctgc cccctagtgc tcacgccata gcacctgagg
     4381 ccggcaacgg cggcatggcg gcggaccaag gggtttactt aagctgtgac ccaacttggc
     4441 cgcgtaccga tatgggctgg tttgtggtgc cctggggctt tcgcaagatg ctcaactggg
     4501 tacaccagcg ctacccgaat ttgcccattt acgtaacgga aaacggctgc gccaaccccc
     4561 accctgagga agagaccagc caggcggatc gcttccgagt ggacttttta cagggctacc
     4621 tcggcgcctt agaacaggcc cgcaagcagg atggcgtgcc ggtacagggc tatttttgct
     4681 ggtcattgct ggataacttt gaatgggcct acggctacag caaacgcttt ggccttatcc
     4741 gctgcgattt tgccagcggc gcgcgcaccc ccaaaaacag cttttatttt taccaacact
     4801 acctgcgcca acacctatga gccaagccgc tgatactccc tccccctgca tgagttgcgg
     4861 cgcctgctgc gcctattttc gcgtgtcgtt ttattggggg gagcagcaga gcgccggtgg
     4921 cctagtgccc gatgagctga cgcaaaaaat cagcccccat ttaagctgta tgcaaggcac
     4981 cggcagcaaa cccgcccgct gcgtggccct gacgggggac atcggccaga gcgtggcctg
     5041 cagcatctac gcgcagcgct ccactacctg ccgggagttt gccatgagcg gcgagaacgg
     5101 tgagcacaat ccggagtgcg ataaggccag ggcctttcat ggcctggcgc ctttggtgtg
     5161 gcgggtttaa ttcgccccct tcggaggcga tgccattaaa ttaagcgttt tgtagccttg
     5221 tttacttgct gtgcgcatgc ccgcaaaccc caccccagcc ctccccttta aaggggaggg
     5281 agcagaatta ccttggacgc cagcaaaatg cctcggtttg aggtttttac tctccccttg
     5341 caaggtaatg ttattaagtt tatgactttg acccaacccg aggctctgcc ctcagcacag
     5401 gacaattcgc aatttttccc acgcgaacct attaggcaaa aagtgcgcta atcaaattgt
     5461 ctaagggcct tacggcaaat tagccacaat cggcgggccg attatttttg taagccagac
     5521 cggtagtttt ttccaaatat taattattaa tttgtatttt ggattatcgg ggtttaaccc
     5581 cggcatagcg ccgcctggcg gcaacagata ataccaataa tggctatagg gctcggcacc
     5641 ccactggcgt ttgaatttaa acgtgcccgc atcctgggtt gagcggccga aatcaaaaaa
     5701 ctgataaccc tgttcaatag caaaaccgag tatttgccgg tacatccaca tattggcatt
     5761 ccagccgttg gctttgcgca ccgtggacgc ccagggaatt tccagcatat tgccgtggcc
     5821 gattaaaaac ccagccgaca ccggtttgcc gtacatgtgc accagggcga tatggcaatg
     5881 caaactcggc tcctccagca gacgtgcaaa ccaacttttg gcgtacaccg gcgtgcctaa
     5941 atcccgcata ttttgcgcaa atacactgta gaactcggct aataattcac ttttgccgat
     6001 ggtaaattgc ggtgtgtgct ccagggcttt tttgtattgg gcgcgcactt tggcgcctaa
     6061 ttgctgctcc aactgcgact gattcgtggg cagaggcaaa attaatgagg cttttttgct
     6121 ggagcagtgg ccgttcggca tgaggccctg ctgcatacaa cgcacttcaa tatggcgcaa
     6181 attaagctgc tcacacaaca cggcggcggt ggcaattaat tcctgggcca cattaaaaaa
     6241 ttccgacaat acgccgccat aattaaaata gggcaccgaa accgcaaagc ggccaaataa
     6301 ttggctggca aaaatcacca aaggaatgcc gccgataatg cgcccctgct catcaaaggc
     6361 cacccaaata attccctcgt ggccaaattg ttcttttatc agctgcagcc aaatggcgta
     6421 gtggtaatta ttagccgagc agacttttgc cacgtaagca tcccacagcc cccactcctg
     6481 aggtgcaagc gcccggcagt gaaagggtgc taagtggcta gcggtgttat caatcagggt
     6541 cagggccggt aggggctggg gtttagcctg aggctccggg ggcgcctggg gggcgctaag
     6601 cgctgttata tccgcctcca gagcttttaa ttccgccgtg atggcctgca ttgcggccag
     6661 cgccgcgtga tgctctgggg tgcccgcggt gagggtttta aattggcggg cgatctcacc
     6721 tttgcgctgc ttggtggctt ttagctgtgc gctgagggat tgagccgaaa gtagtggagt
     6781 ggcggtcatg gattaagctg caagtatagg gcctccattc tagccagttc tgccccgcct
     6841 tagctaatcg gcttgggcca cgcagcccat ttaggccatg cggccgcgca ggtattcaaa
     6901 ccaggaaatg tagcgcacga agtggttgag ctgctggggc atgccgatgc gctgaatgcg
     6961 atacggcgct tgctgaatgg cctgggcgca ggtgtttttt gagagagtgg agacggcggc
     7021 taaaaaaccg gcctgctgca ccaatggctc atggtgggat gcaaaatcat tttgggtgcc
     7081 ggaggggtag cagaaaacct ctgacggctg cacaagctca gcgcataagc gcgccttggc
     7141 gtgggccaac tccgcagcca cctccccatc gctcagggcg ttaaaggtga aatgggaacg
     7201 ggcgtggcag ccgatacgca ggcccgccgc ctccgcccag cgcaactgct cccaagtggc
     7261 cgcttgatat tctggcgggg ccgcctgggg aatgctaaac cccacgcagg cctgcagttg
     7321 cccaagcaaa gcctctatgg ctgggcgctg cagggctttg gccaaacggg ttaactggcg
     7381 gcgggttttg cggcgcgcct ctgggctgga taaatccacc gcgacagcct ggccggacag
     7441 ctccagagaa tacaggcccg ccggggcgtg ccagcacaaa tgggccagct ggttatccca
     7501 gggccaggcc gcgccatcga ttaggtcggt gatgacaaac agtgtcggtt tagcttcata
     7561 acgcagtaaa accggcacca gctgctccag ctgatcggca aagccgtcat ccaaggtgaa
     7621 acacaaacaa cgccgcaggg aatcgcccct gagcgcgcgg gccactaatt catccaccga
     7681 gacaaattca tagccccgcg ccttaacaaa acttaacgcc tgggcaagaa attccgggct
     7741 gatgccctca taggcgccgc tggcggcagg cgggcgatgg agggtaaaaa tggtcgccca
     7801 actgccgagc agccctgtga gcaagcgctc aatgcggggg tcggtgtaga tgtagtctgc
     7861 aagattcgac acctagaact ccttacatcc aagcagacaa acgcgcccca aaggcgacgg
     7921 gtgttgcgct gatatcattg tgtaagccga tacgcttaag ctcatgcagg ttttggccca
     7981 catgggctat gccgcgctga gttgtcacgg cggcttggta ggtttcctcc actatctgca
     8041 gggcggtcgg tgaataatcg ccgttgggaa aacaaaataa gttcacggct cgccctaaac
     8101 gctcttgtaa aagactctga ctgtgttgaa tctcatagcg tagctggctt tcatccaaag
     8161 cgctggtcaa gcgccggtgg gtacaggtgt gggagccgat ttccacccaa ccgctcgcct
     8221 gcatctgggc aagctcatgc caatccatca aagccctcgg caggggcgcc gctaagctct
     8281 gcccattgag ctgtgcaagg gcggtaaaaa tctcttgatc ggggtagcgc tttaaaaagt
     8341 ggatagcaag ggccaggcgc tcgcgattca cacccgtggc aaggcctggc accgcctcgt
     8401 gtaacgcctg gaaaatgggg tgcgcctgca actgcggcag tgcgccctgc aacaataaat
     8461 tcgcaactag attgggccag aattgaaagt cggtgccgat tttctccgcc accgcaaaca
     8521 gggtcgcagg cacacgggcc tgctccaaca gcggcagcgc gaactcgaag ttatcccgcc
     8581 agccatcatc aaaggtgacg gcgcaggccc gcgcaggcaa ggcctcgccg cggcggcttt
     8641 tagccaccca gtcagataaa aacatcgggg tgaaatagcg ccgcagctgc gccagatgca
     8701 tggcgaacac ctccggtgtt acacacatac cgggctcttc tgccttacag cgggcatcat
     8761 cggcggggag aatgcggtgg tacatcaaaa cccacagctg gggtgcacct gtgtgccagc
     8821 gctgcggccc ggcggcaaag gccgcctgtt gggcgaggga tttgatgaat ggcttgagca
     8881 tcacgaacct tgtggatcaa aacgggctac tgcagaaccg ttaacttggc gcaaaaatag
     8941 gcaaacatca agaaaaaact ggcccgaaag accagtttgg ggaaaacctc acagcctgcc
     9001 cagaggcggt gaggcgcctg agcagggatc tatttcccgc ccttagcttc ctcaagatgc
     9061 ttgagaatct ccggcaggtt gggggctagg gttttggcct tttccagcca gcgaatcccc
     9121 tcagctttat tgccggtttg caccagaatc cagccgtagg tatccaacac ttccgccgca
     9181 ttcggcgcca gtgcaacggc ccgtttcgcc gtctctatag cgcgggcgtc tttggtttca
     9241 aaatacagcc aggctaaatt attcagtgcc aaaaccgatg gcgcctgtag ctgcagcgct
     9301 tgctcatacg cgctgatggc cgccgctttg tcgcctgcgc cttgggccgc cagcgcttgg
     9361 atgagcagtg gacgggcgct ttgcggcagc gctttgcgcc attgggctaa aaattcaacg
     9421 gctttggcgg catcattgga attgagaatg gcataaatta attccccaga tttatccatg
     9481 ggctgcgcgg taaatgacac ttgcaataag gccagcgcct cggctttttt cccttccgcc
     9541 aaaagcacta agccattgag aaaattcagc agcgcaggct cggcattgcc cttggcatat
     9601 tcggcgataa tggcccgcgc ctgagcaatt ttttcatccg ctaattcaag ctccaccaaa
     9661 aacgtcagaa tcgcaggatt ttccggctgt aatttaagcg ctttattaat ccaatcgcgg
     9721 ctgtgcaaaa tatcggagga ttttaatagc tgggctttct gcgtgtataa ctgcaccaac
     9781 agctgtttga tactgggcaa ctgcatggct ttgcggtgaa cttcttcggc gttcacaatg
     9841 gctttatcaa tatcgttatt cgcggcaaac cattgcgcca atacccaatt ggcttgccag
     9901 ttttcatccg tgcggctttg cgcggctaaa aatacttccg cctgttctaa ttggtgaacc
     9961 tgtttggccg ccgctaaata atgctcataa gcaggaataa aaccgggggc cgcggcaatg
    10021 acctgctgcc aggcgggaaa tgcccgcgcc caatccgctt gagcttgaaa cacctgtgcc
    10081 gaaccggcca agggcaggta tttctcagcg ccggacagcg gcaccgcctg ctcaaacgcg
    10141 ctcaaggctt gggggtaatt cttttgctgc agtgccagcc aaccctgcca aaaatatttg
    10201 cgcccagcca aagactccgg cagtgaatta ataatttggg taataaaatc cagctgttta
    10261 ttggcaaaca acaactgaaa taaaatctgc tgagtttcaa aatgctgggg atcttgcgcc
    10321 agcgccgtgc gcaattgcgc caccgcctcg tccattttat tttgaccggc aaaataacgc
    10381 gcctttgcca tataaatttt tggttgattt tgttgcagct gtaaactttt atccaaccac
    10441 tcaatcgctt cagcattggc ggggtcttta tccaacagcg caatcccgta agtggccaaa
    10501 atctccgccg acttaggctg attctgcgcg gcttttttta acagctccag cgcctcatca
    10561 atttgttggt tgcgaattct gctgagcgcc gccgcctgca gcacccgcgg cgcagcggtt
    10621 tccgggtcga tggtcgcatc aaaaattgcc tgcgcctgtt tggtatcgcc ctgctgtaac
    10681 tgcaccaggc ccaataaggt attggtattt ttatcctgcg gaaattgttt ttttaattca
    10741 ctaatcaccg cctgggcctg atcaaaatca cccttttggt acaactgcag cgcctgatta
    10801 aacttctgtt gcgcagcggc gccctcgggg ttcgcctcag cgagcatttt ttgatagtgg
    10861 tatgcctcgc tggagcgccc taggcgggtt aacacttccg tcagtaaatt taatacacgg
    10921 gctttagcgg cacttaacat atccccggtg ggcactaagg cgagcgcttt acttaaatag
    10981 gcttcagcct cctcaagctg gttggcggtt aaggctattt gccccaataa aacccaggct
    11041 tcttgatcct gcggtttctg ctcaacccac tgtttcagca gcgcctgggc gccactccaa
    11101 tccccctgag ctgccattaa ctgggcgcgc caaaaagcta ctgcggcggg gctgtcagcc
    11161 gtctgttcca actgggataa atgttcctgg gccagggcaa aatccccctc aagcgcgcct
    11221 acctgtacca ttaactggtg aaattcggct gaattgttac gcacagccgc gggcaaaccg
    11281 agcaatactt tttgcgccga ctggtattta cgctcggcaa ttaatgcttt tgcccactgt
    11341 atattaagcg ccaaatcatc tcgggcaatt ttttccagca attccgccgc tgcctgattc
    11401 gcgccgattt gcatataaat atccgccagc aaggctaaac cttctaccgg ttgttccgct
    11461 ttactggcgg cgttgcgcgc ttcgatcata gcagcccgcc actggccctg ctgctgataa
    11521 gcgcgcgccg tgtttaaatg ccgttgtgat tcatcgacct tttgtttcgc gctgcgatca
    11581 caggcgctca agcaaagtaa tggtgtgata accaataaag cgcgcatcaa tccgttcatg
    11641 tttattatta acctggccag catcaaaatt gatagcgaac ggaaactccg gtgcgcaatt
    11701 catcgtattc gcttaaggca tcatcggaat ggcgcttaat ggtgccaagc aaaaaatccg
    11761 cacttaaggc tttagtaaaa tcgtgagaga tacgcagcag aacattatct tcctggtaat
    11821 cgctgagttt tcccacctga tcaaacctag attcactgtg ctccaagcgc aaattaccat
    11881 gggtgcgagc agcaaagctg taattgatcg atagtgacac agcgcgatcc gtataatctt
    11941 ccggcaaaac ataataatca tgattggaat attgcgcctg cgcacttaaa cgcaacccgc
    12001 gccacagttt tggccccgtc caactcagat tggcctggga gagtttcacc aaatcgtaat
    12061 tttggctgag cgaatcctgc atcagagttt catccaatga agcgccgttt ccatcaccaa
    12121 ataaagtatc cgtaacggcg gagctggcgc ctatagatag gttgtggcca tttttatcgt
    12181 aactgatatc aagattgtaa ttgggtttgc caaattcctc ttggccccgc gccttagtat
    12241 tttgataacc aagctgaatg ccgtaggcca aatgacttaa ctgagcttga taacctaggc
    12301 gagcaatctg atatttataa tccgcctcct ccgcatcctt gcgcgtgctt tctccctgat
    12361 tgatcgatac caataattga tccgtggcac ttaaactatg ttgccaaacg aggtcggcgg
    12421 tatcacgatt ggcatcagcc gcagccaagc gttttaatcg gacggcttcg cggctgacgc
    12481 caagaataat ttgatccacg ggcgagacgt gcaaacgcaa ttgaggcttt agcgtcaata
    12541 attcccgctc cccggtgtta cttaacaagg gcgtagcaac attggagcgc agcacctttc
    12601 gtctggagtc ctgcagggtt aaattgaacc aatcgtactc tttaccgagc acccactggg
    12661 catcaccctc gagggtattg gcgtcttctt gggaggcttg cgaaaaatag gtgcgatctg
    12721 ccgagtaacc cagtttgaaa ctactccagc gatttatttg ctggccactt aaccctaccg
    12781 aaagcgtatc ctgccgttca tttaatccat cacctgaggt ttttaatgca ttgtcggtga
    12841 agatactctc catgctcaga tcggcactaa agtgatcctg ggctggcgcc ccccaagcac
    12901 tcacgcaagc gctacacaat aagctcaagg ttatcgatcg ctgatacacc ataattccaa
    12961 ccttaactat taaataccag gcctgcaaaa tgctctttgc ctagggcgtc gatggccgat
    13021 aacacttcat cggttactac tttgccaaag ggcaccacca acatactaaa atcacaatac
    13081 tgcgccaaaa tgcgcgcctc agtggactgc tgaatagccg gcgcatctaa aacgataaaa
    13141 cggtcggagt agcgctcttt tatttccgcc accaaaacct ccatgcgttt agaattaaaa
    13201 aattctgccg ccgattcact ggagccgccc gaggggataa cgcgcaagcg ttcaatacct
    13261 ga
//
LOCUS       DAOQGS010000037        13249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2176_length_13249_cov_4.594285, whole genome shotgun sequence.
ACCESSION   DAOQGS010000037 DAOQGS010000000
VERSION     DAOQGS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2176_length_13249_cov_4.594285"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PKZ52_02465"
     CDS             complement(<1..935)
                     /locus_tag="PKZ52_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNG58659.1"
                     /translation="MLKSRAAIATAPGQPLTVTEVDVAPPKAGEVLVKIIASGVCHTD
                     AYTLSGSDPEGIFPAILGHEGGGIVVEVGAGVTSVAVGDHVIPLYTPECGECKFCLSG
                     KTNLCQKIRATQGKGLMPDGTSRFSLDGKPIFHYMGCSTFSEYTVLPEISLAKVNKAA
                     PLKEVCLLGCGVTTGMGAVLNTAKVKPGDTVAIFGLGGIGLSAVIGATMAKAGRIIAI
                     DINERKFDLARKLGATDCINPSHYDKPIQEVIVDLTDGGVDFSFECIGNVNVMRSALE
                     CCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRG"
     gene            1190..2017
                     /locus_tag="PKZ52_02470"
     CDS             1190..2017
                     /locus_tag="PKZ52_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNG58660.1"
                     /translation="MRVWTFANQKGGIGKTTTCVALGGLAADLGLRVLMVDLDPQGSL
                     SSYFKLNPDQTAASSLSLFQERKQLSADFIGQLIINTPFKNLCLLPASMGLATLERQA
                     IGPEGMGLVIKKSLDLIADDFDLALIDCPPALGVLMVNALAACDLLIMPVQTEFLALK
                     GTERMLNALKMINRSRSSQMPYLMVPTLYDRRTSASVTSLRTLRHTYGNAVWPGRIPI
                     DTRLRDASKMGLPPHLVAEESQGIDSYRSLLGYLLPQKELKALRAQASTAWPTAPRI"
     gene            1993..2922
                     /locus_tag="PKZ52_02475"
     CDS             1993..2922
                     /locus_tag="PKZ52_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58661.1"
                     /translation="MAHRAEDLIGQYLTDLFAPAAEAAAPERARPVEPAPAEKPEPLT
                     PGPSPAALKPIQASAGDKHPPQAPVYSQASPANLPSPAEVTLAEPARQQLQQLLNSAW
                     QAPVQVQDPVQGQSQAIAPVAPVAPEPTPPIAPIDPPAVAAPHINASAVAGKPAWATK
                     TFDTLLVDVAGLKLAIPLVVLGHIFQVQDGFSHLPGQGPLFLGTLNTPRGPLRVINTA
                     LLVMPERYEAAFVEQVQFVVSLAAESAKGQPWQLALAVNRVAQPVSLACDDVVWRGER
                     SKRPWLLGTVKSQMCALLDVEVIGQMLVGAASA"
     gene            3006..3503
                     /locus_tag="PKZ52_02480"
     CDS             3006..3503
                     /locus_tag="PKZ52_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNG58662.1"
                     /translation="MATNVSASLSKSKTSDDPILQWVTFHLAGETYGVNVMQVQEVLR
                     YTEIAPVPGAPSYVLGIINLRGNVVTVIDTRHRFGLEPGDMTDNTRIVIIEAEKHVVG
                     ILVDSVAEVVYLRQSEVESAPNVGNEESAKFIQGVCHKNNELLILIELNKLITDEEWG
                     EIGAF"
     gene            3543..3950
                     /locus_tag="PKZ52_02485"
     CDS             3543..3950
                     /locus_tag="PKZ52_02485"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNG58663.1"
                     /translation="MDPWVSGLLILSGLSAFGALAVLFVKKQQKLSGEITKCLMQMEA
                     MEKSIAMQNSIAIGMGQRILSLEQSLKAASSARGEPAAAEDFSYTQAMQMFDRGLDAA
                     TVSASCGISRSEAQLMELIRKQGKAPRFTSEPV"
     gene            3947..4462
                     /locus_tag="PKZ52_02490"
     CDS             3947..4462
                     /locus_tag="PKZ52_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58664.1"
                     /translation="MMRWLYALITAQCLSGCTLYSSDHQTQFADAEGRVPAQLFSRLG
                     QQSASKPWVLKQLGQPMAVNSLGQGAQVYTWALTRNEISERSFFLLYKSKHTKNQQNF
                     LHLVYSGDELLKHWLDRDAVVDTKALLNSRDLQRLAVSNLEKTASAPSNYSPPERPVV
                     TRITPATDSDE"
     gene            complement(5034..5753)
                     /locus_tag="PKZ52_02495"
     CDS             complement(5034..5753)
                     /locus_tag="PKZ52_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58665.1"
                     /translation="MINIVFEAIEQHKSTLIDHPFCQNLRSYSDNNIDAYRFVPHMAF
                     FVLGFKDMLKNLQVNYPKNPVDHMLNHHCHEDSDHWQWFLEDLKTLKLGVEKWGGDIG
                     GALALIWSDADFPVRNQVYRVMHHLFQCRSTHEKLILVECLEAAFAAFVVNLNYLTQR
                     SGHFKTLKYFGEHHFTEESSHAMGSWLNEGGKAPVAPDNAHQIRTDTMLAMVDDIFAG
                     FNQVFTIWDKAWVEETILEAA"
     gene            6935..7516
                     /gene="sodB"
                     /locus_tag="PKZ52_02500"
     CDS             6935..7516
                     /gene="sodB"
                     /locus_tag="PKZ52_02500"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HNG58666.1"
                     /translation="MAIELPALPYAKNALAPYISEETLEFHYGKHHQTYVDKLNGLLP
                     GSEFEGKSLEEIVKTSSGGVFNNAAQIWNHTFYWHCLAPNAGGEPTGAIADAIVASFG
                     SFAAFKEQFNTSAVNNFGSSWTWLVKKSDGSLAIVNTSNAATPLTDASTKPLLTVDLW
                     EHAYYIDFRNARPKYLEAFWALVNWDFVNKNFA"
     gene            7617..8354
                     /locus_tag="PKZ52_02505"
     CDS             7617..8354
                     /locus_tag="PKZ52_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58667.1"
                     /translation="MIERREPTLSGRTEADEPLTRIPANTRPSSASAGRAPVSTGSSP
                     LAPFAVLLALASLALGAFFYWQLTQFQQQAVQQASLLTAAENRVSELEKRLNSTGETS
                     EQSLAALQDKLKTNANEVAKLWDAQNQLKSKTTAALEETNTTVNKQIQAALRDLEGKS
                     KQAVTDLQSELKVLQDLVQAQQSSSSRDDEARKELASKVSDLSKRLKAVESDLGERVR
                     NTENAIESIDAFRLQVNREINKLKGGQ"
     gene            9890..10198
                     /locus_tag="PKZ52_02510"
     CDS             9890..10198
                     /locus_tag="PKZ52_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNG58668.1"
                     /translation="MSINSSLSSDGRTLTIAVDGRFDAGSLDEFRSCYESAVRDGPIR
                     FNVDLKRAEYLDSSALGMLLVLRDYAGGESASITISNCSPEVKKIFAISSFEKLFTIQ
                     "
     gene            10250..11968
                     /locus_tag="PKZ52_02515"
     CDS             10250..11968
                     /locus_tag="PKZ52_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNG58669.1"
                     /translation="MSKKIPQSPIKVLIADDSPVDRFILQTMLERLGYTVVLAEDGQQ
                     AIEQFLVEKPDLVFLDVLMPNVDGVEAASSIKEAAGEDFIPVIFLTSLVDAPSLARCL
                     DAGGDDFLSKPYNQIIIEAKIKAFSRMRVMNRTLSLQKNLIERNNKHLLQEQTVAKQV
                     FDRIANTSSLNLSIIRYYMSPMAVFNGDVLLADISPTGSLMVLLGDFTGHGLPAAIGS
                     MPLASIFYGMVNKGFGMSEILREINGKLNKTLPMGLFCCATFLDINLRKKRLLVWSGG
                     LPDGAIYRAGGKTVERLASRNLPLAILSNQSFKAEAVRYVLEPGDRVLLWSDGIQEAR
                     NTEGVMFGEERLNALLEQELDNIHIFDDILQKVQHHIGEGERDDDVSLVEVRFPDEKE
                     AAEVEKQGSRGRGCLQDWSLDFRLEASSLRVFDPMPLLLHITSEVPGLRQSNSVLFTV
                     ISELYNNVVEHGVLGLKSSIKQSPQGFAEYYRLRKEGLENLPKDCFVILEFSHKVDPG
                     GGVLRIRVRDSGAGFCPDETVATPGGVPIYHGRGFALLRSMCESITVLPPGNCVEAQF
                     RWLAED"
     gene            11973..12323
                     /locus_tag="PKZ52_02520"
     CDS             11973..12323
                     /locus_tag="PKZ52_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNG58670.1"
                     /translation="MAADASLNAEMLDELRELLAENFNELINRYIADSQTRFNTLHTA
                     IAQADFKVIYYEAHGIKGSSRNMGANILAEIMARLEALGQSQNPQGLAEVFADAQAEF
                     AKVVVGLEAFRTAE"
     gene            12489..>13249
                     /locus_tag="PKZ52_02525"
     CDS             12489..>13249
                     /locus_tag="PKZ52_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNG58671.1"
                     /translation="MPTIVRQDDIIASVADALQFISYYHPIDFIQALHQAYLREENPA
                     AKDAMAQILINSRMCAQGHRPICQDTGIVTVFVEMGMNVLVEGTMSFDDIINEGVRRA
                     YMQPDNILRASILADPDGARKNTGDNTPAVIHYRLVPGDTIDIHVAAKGGGSEAKTKF
                     AMLNPSDSVVDWVLSVVPQMGAGWCPPGMLGIGIGGTAEKAMLLAKEALLEPVDIQEL
                     RERGAANRSEQLRLELFDKVNALGIGAQGLGGLTTV"
BASE COUNT         3155 a         3153 c         3469 g         3472 t
ORIGIN      
        1 ccccgccata cccggccggt gactaattga aaggggcggg tgctgatttc ttgcccggcg
       61 cctgccacgc caataattac cgactccccc cagcctttgt ggcagcactc taaggctgag
      121 cgcatgacat tcacattgcc gatacattca aaggaaaaat ccacgccgcc atcggttaaa
      181 tccacaataa cctcttgaat gggtttgtcg taatggctgg ggttgatgca atcagtggcg
      241 ccgagtttgc gggctaaatc gaacttacgt tcattaatat cgatggcgat aatgcgcccg
      301 gccttggcca tggtggcgcc aatcacggcg gataaaccta tgccgcccaa gccaaaaatt
      361 gccactgtat cgccgggttt tactttggcg gtatttaaca ccgcgcccat gccggtggta
      421 acaccgcagc cgagcaaaca cacttctttt aggggcgcgg ctttattgac tttggcgagg
      481 gaaatttccg gcagtacggt gtattcagaa aacgtagagc agcccatgta atgaaaaata
      541 ggtttgccat cgagggaaaa acggctggtg ccgtcgggca ttaacccctt gccttgggtg
      601 gcgcggattt tttggcacag gttggttttg ccggataaac aaaatttaca ctcgccgcac
      661 tcgggggtgt agaggggaat tacatggtcc ccaactgcta cggaggtaac gcctgcgcca
      721 acttccacca ctatgccgcc accttcgtgg cccaaaatcg ccgggaaaat cccctccggg
      781 tcgctgcccg atagggtata ggcgtctgtg tggcacacgc cgctggcgat gattttcact
      841 aatacctctc cggccttcgg cggcgctaca tcaacttcag tgacggtgag gggctggcct
      901 ggggcggtgg caatggcggc gcgggattta agcatggggc ggattccaga tggggttaaa
      961 cctaaaaaat caggccttgc ctaggacgaa tccgtagggc gtggatctgc acagttttta
     1021 ggtggaagcc cggattatgc ctagccttgg gttgctttaa aaggcgcgga ttgatgaggg
     1081 aactgcgcgc tttggctttc aaaccagcag ctgcttagaa gataaaaatt cccaccgtat
     1141 gaaaaaccgc aggctatggt ttaaggagaa taatgaagca ggtgatgcta tgcgtgtgtg
     1201 gaccttcgcc aaccaaaaag gcggcattgg caaaaccaca acctgtgtag ccctgggcgg
     1261 tttggccgct gatttagggc tgcgggtgtt gatggtggat ttggaccccc aggggtcttt
     1321 aagcagttac tttaaattaa acccggacca aaccgccgcc agcagcctaa gtttgttcca
     1381 agagcgcaaa cagctgtcgg cggattttat cggccagctc atcattaaca cgccgtttaa
     1441 aaacctctgt ttattgcccg cctccatggg ccttgccacc ctggagcgcc aagccatagg
     1501 ccctgaaggc atgggcctgg taattaaaaa aagcctggat ttaattgccg atgattttga
     1561 cctggcactt atcgactgcc cgcccgccct gggggtttta atggtgaacg ccctggccgc
     1621 ctgcgattta ttaattatgc cggtgcaaac agaattttta gccctcaaag gcaccgagcg
     1681 catgctcaac gccctcaaga tgatcaaccg ctcccgcagc agccaaatgc cctatttaat
     1741 ggtgcccacc ctctatgacc ggcgcaccag cgcgtcggtg acgagcctta gaaccctgcg
     1801 ccacacctat ggcaatgccg tttggccggg gcgcatcccc attgataccc gcctgcggga
     1861 cgccagcaaa atgggcctgc cgccgcacct ggtggcggag gaatcccagg gtattgatag
     1921 ctaccgcagc ctcttgggtt atttattacc gcaaaaagag cttaaagccc tcagggcgca
     1981 ggcgagcaca gcatggccca ccgcgccgag gatttaatcg gccaatactt aacggacttg
     2041 tttgcccctg ccgctgaggc cgcagcgcct gagcgcgccc gcccggttga gcctgcccca
     2101 gcagagaagc cagagccact cacaccgggt ccatccccag ccgcgctgaa gccaatccaa
     2161 gcatcggctg gggataaaca tcctccgcag gcgcccgtat attctcaggc ttccccggcg
     2221 aatttgccta gcccagcgga agtcaccctt gctgagcccg cccgccagca gctgcagcaa
     2281 ttgctcaact ccgcctggca ggcgccggtt caggttcaag acccggtgca ggggcaatcc
     2341 caggcgattg cccctgtcgc tccggtagcg cctgaaccta ccccacccat tgctccgatt
     2401 gatcctccag cagttgccgc gccgcacatt aacgcctcgg cagtcgccgg caaacccgct
     2461 tgggccacca aaaccttcga caccctgtta gtcgatgtgg ccgggttaaa gctcgccatt
     2521 ccgctggtgg tgttggggca tatttttcag gtgcaggacg gcttttccca tttgcccggg
     2581 caggggccgt tatttttagg caccctcaat accccccgcg gcccgttgcg agtgattaat
     2641 accgcgttat tagtgatgcc cgagcgctac gaggccgcct ttgtggagca ggtgcagttt
     2701 gtggtgtccc tggcggctga gtccgccaag gggcagccct ggcaactggc gctggcggtt
     2761 aaccgcgtgg cccagccggt aagcttggcc tgtgacgatg tggtctggcg cggggagcgc
     2821 agcaagcgcc cttggctgtt aggcacggtt aaatcacaga tgtgtgccct gctggatgta
     2881 gaggtcatcg gccagatgtt agtcggggcg gcaagcgcct aggccaaact ggcgcgccct
     2941 gcacccttga ctatagttaa gaaggatttt ttgaattttt aagtgccgcc aggcaagagg
     3001 attttatggc gaccaacgtc agcgcaagtc tcagtaaaag caaaaccagt gatgatccaa
     3061 ttctgcagtg ggtgacgttt catctcgccg gggagaccta tggcgttaat gtaatgcaag
     3121 tgcaggaagt gttgcgctac accgaaatag ccccagtgcc cggcgcgccg tcctatgttt
     3181 taggcattat taatttgcgc ggcaacgtgg ttacggtgat tgatacccgc caccggtttg
     3241 gcttggagcc gggggatatg acggataaca cccgcattgt gattatcgaa gcggaaaaac
     3301 acgtcgtggg tattttggtg gatagcgtcg ctgaggtggt gtatctgcgc caaagcgaag
     3361 tcgagagcgc gccgaatgtg ggtaatgaag aaagtgcgaa gtttatccaa ggcgtctgcc
     3421 acaagaacaa cgaattatta attttaattg aattaaataa actgattacc gatgaagagt
     3481 ggggcgagat aggcgcgttt taacctgtat cgcctctaag ctgttttgag caggagttga
     3541 ttatggaccc ttgggtcagc ggtcttttaa tcttaagtgg cctaagtgcg tttggcgcac
     3601 tggcggttct atttgtcaaa aaacagcaaa aactatccgg cgaaatcacc aagtgcctaa
     3661 tgcaaatgga agccatggaa aaatccatcg ccatgcaaaa ctctattgcc atagggatgg
     3721 ggcagcgcat tttatccctt gaacagagcc ttaaagcggc cagcagcgcc cgtggcgagc
     3781 cagccgctgc agaagatttt agttacaccc aggccatgca aatgtttgat cgcggcttag
     3841 atgcggcgac ggtttccgcc agctgtggta tttcccgctc cgaagcacag ctgatggaat
     3901 taattcgcaa gcaaggtaaa gcaccgcgct ttacctcgga gccagtgtga tgcgttggtt
     3961 atatgcgctt attaccgccc agtgtttaag cggttgtacc ctgtattcaa gcgatcatca
     4021 aacccaattt gccgatgccg aggggcgcgt gccagcgcaa ttattttctc gtttggggca
     4081 gcagtccgcc agcaaaccct gggtattaaa acaattgggg caacctatgg cggtgaatag
     4141 cctaggccag ggcgctcaag tgtatacctg ggcgctcacc cgcaacgaaa ttagtgagcg
     4201 gtcgtttttt ttgttgtaca aaagcaaaca cacaaaaaac cagcaaaatt ttttacactt
     4261 ggtttatagt ggtgatgagt tactcaaaca ctggttggac cgcgacgctg tggtggacac
     4321 caaagcactg ttaaatagcc gcgatctgca gcggctggcg gtgagcaatt tagaaaaaac
     4381 cgcttctgct ccttctaact attccccacc cgaacgccca gtggtaaccc gcattacgcc
     4441 ggcgacagat agcgacgaat aggttgggtg gcgcactcgc tgtatattta agcactatct
     4501 gaaaaatagg cgctgccaaa aatgctcgat gctattacag ggcatatttt tattgttgct
     4561 ggcagtgcct catacgcgcg atgcctgtgc catcaaaacc ttcctgccta tcggtgccta
     4621 agtattgcaa caggctgcta gcaattgttt atcactattt agcacttttt taaccaagat
     4681 aaaaatccgt ccaagtccct ttaataaaca ctccagcgcc tcgctagggc tcacatcttt
     4741 gcaaaacacc gcaagtgaca aaaggcaaat aactgaatag ttacgattat tttaattaat
     4801 tttcgcacaa gctatttctt tggtgtgcac ctggctgccc aggttggagc cctgcagttg
     4861 cttatattgt gggatattcg tgcgggcgcc caaggggttt acttgtgatg cttgagaatc
     4921 ctcagcttaa ttgagcctaa tggtgtggca ataacccaca aagcctagcc tctgttgccg
     4981 tctatttatc gtaatcggct gttagagcct agctttgcgg gcgaagggat ttattaggcc
     5041 gcttctagaa tggtttcttc cacccaagct ttatcccaaa tagtaaatac ttgattaaat
     5101 cctgcaaaaa tatcatccac cattgccagc atggtgtctg tgcgtatttg atgggcgtta
     5161 tccggcgcca cgggcgcttt gccgccttca tttaaccagc tgcccatggc gtgggaggat
     5221 tcttcggtaa agtgatgttc gccaaaatat ttaagagttt taaaatggcc agagcgctgg
     5281 gttaaataat taaggttgac cacaaaggcg gcaaacgctg cctccaggca ctccaccaat
     5341 atgagttttt cgtgggtgct gcggcattga aataagtgat gcataacccg gtacacctga
     5401 ttgcgcactg gaaaatcagc atccgaccag atcaaagcca gcgcgccacc aatatcgccg
     5461 ccccattttt ctacacctaa tttaagcgtt tttagatcct ccaaaaacca ttgccagtgg
     5521 tcactatctt cgtggcagtg atggttaagc atgtgatcga cagggttttt ggggtagtta
     5581 acctgcagat tttttaacat gtctttaaaa cctaaaacaa aaaatgccat gtgtggaaca
     5641 aaacggtagg catctatatt gttatccgag taagatcgta ggttttggca aaaagggtgg
     5701 tcaatcagtg ttgatttgtg ttgttcaatg gcttcaaaaa cgatattgat catattaact
     5761 cctaaaaaag gccagaaagc gcattaaaat cagttatcta tctcgcgctt aactcaatta
     5821 gagcccgtgg cacttatcat cgttgtgcta ttgggtgccg tgctctagtt aaaataaagc
     5881 ataagactct ggcactactg atattgattc gaaataggat ttccctaggt ttaattatat
     5941 tttgaagtaa aggctatagt gcggcaagag tggtgattgt tttttttaag caaaaaaagc
     6001 aatagtgcgg cgacttaatt ttgactttat ttatattttt tctgccgttt gggcggttag
     6061 ataaacatgt taaatctatt ggtgctctat gcgcggtatt gggtttgcta tttgatttgg
     6121 attatttatg cgcaacaaat ctaaaggtgt tctgcgttgt tcggttgtta ttaacccggc
     6181 ggcaaaatag atcgccctgg tctattttgc cgcccggtta ataaagtgcg tagagggaat
     6241 aggcttgtca cttccgttcg gatcagtcag ttacatctgg gatgttaatt tgcgctcacc
     6301 cctaaaaatg cctgtcatca tcataaaagt gaaataattg cagtgctcat ttgccgaata
     6361 gaaatgattg agccggtacc cggcatttaa gcaagccaac aattggctgc ttcatttgca
     6421 accattaaat atggtggggg ccgagcaaga tgtcgtttta tcctaaaaac cgcagtaggg
     6481 tttaaccgtg gtctttaggt ttattggcag gctggtggtt gagaggccgc ttggatccgc
     6541 tgttagccag ggctcaccaa tatgccctgc tgcacttatg cgtattttga ttgactgcgg
     6601 gcaggcaaaa tacgcctggt caccccagct agcgaaagtg agcggttgtt gctcctaggc
     6661 gcggggtgcg tagtcagcgg ggcctacttc tggtaaggtg gcccggctgg agtcgggcct
     6721 tttttgtgaa caaggccttg aattggccag cttcggccgc aaattggggg gcaacccatg
     6781 gcgggcttct caaaagtgta tcagcgaccg ttgcgccaat agttgtgcag ccgagcgggc
     6841 gcttcgggcg ggctgcaggg ttttcccatt ttccctcggt gctatacctt ggcaccctag
     6901 ttaccccaac tcccacaaat tggaagatga tcttatggct attgaattac ccgcattgcc
     6961 ctacgccaaa aatgcattgg ctccttatat ttcagaagaa accttagaat ttcactacgg
     7021 caaacaccac cagacctatg tagacaagct aaatggcctg ctgcccggtt ctgagtttga
     7081 aggtaagagc ttagaagaaa tcgtgaaaac atccagcggt ggcgtattta ataatgctgc
     7141 gcaaatttgg aaccacacat tttactggca ctgtttagcg cctaatgctg gtggtgagcc
     7201 taccggtgcc atagcagatg ctattgttgc ttctttcggc tcttttgccg cattcaaaga
     7261 gcaatttaat acctcagcgg tgaataattt cggctcctct tggacttggt tggtgaaaaa
     7321 gtccgatggt agcctggcca ttgtgaatac cagcaatgcc gcgacgcctt taactgatgc
     7381 ctccactaag cctttattga ccgtagattt gtgggaacac gcctattaca ttgattttcg
     7441 caatgcgcgg cctaaatatt tagaagcctt ctgggcattg gttaactggg attttgtaaa
     7501 caagaatttt gcttaatttc aggcgctttg catgaggagg ctttggcctc cttttttctt
     7561 tattgttatt cacacccgcc aatttacact cctgattagt atacaaggct taaagcatga
     7621 tcgagcgtcg agaacccact ctttctggcc gcacagaagc agacgaaccc ttaactcgca
     7681 tacccgccaa tacgcggccc tctagtgctt cggccgggcg cgcacctgtt agcacaggct
     7741 catcgccctt agcaccgttt gccgttttgt tggccttagc gagtttggca ttgggcgctt
     7801 ttttttattg gcagttaacc cagtttcaac agcaagccgt acagcaggcc agtttgctaa
     7861 cagcggcaga aaaccgcgtc agtgaattgg aaaagcgttt aaattcaact ggtgaaacct
     7921 ctgaacaatc cctcgctgcc ttgcaagata agttaaaaac taatgccaac gaagtggcta
     7981 agctgtggga tgcgcaaaat cagttaaagt caaaaacgac ggcggctcta gaagagacca
     8041 acactacggt taacaaacaa atacaagcag ccctgcgcga cttagagggt aaatccaagc
     8101 aagccgtaac cgacctgcag tcggagttaa aagtgctgca ggatttagtg caggcccaac
     8161 aatcttcttc cagccgtgat gatgaagccc gcaaagagtt ggccagtaaa gtatccgatc
     8221 tgtcaaaacg cctcaaagcg gtggaatctg atctgggtga gcgagtgcgc aacaccgaaa
     8281 acgccatcga atctattgat gcatttcgtc tgcaggtcaa tcgtgaaatt aataagttaa
     8341 aaggtgggca gtagcgcgtt ttacctgatt acttcggcag cctattttta gcagcctgtt
     8401 gaaatactac cttgcgagcg ttggcggcgt tataaattga caaaaaatcc tcatttaaag
     8461 cgtgtagact tggcaattgc acttgcattg ctctaactcc tgcattcatg cagtcgtccg
     8521 gttttttgcc aaaatatgcc ttgccagagc gtcgccgcct acgtatttca acaagctgct
     8581 agtgtgttga tacacatgcg ggccagcttt ttaatcgcct tcgatagaca cccctgacgg
     8641 ccgaaactca gcaaatttac cctgacagag cgtcgccgcc gacctattca ccacgctgct
     8701 aaaccaaagg ctgcttgccc tcatggtttt gctctctcaa tcagagtttc cttaaactcc
     8761 gctttttgca gctcatctca cctgtgtgca tacctacgga tgcagcatgc tgtttgctgg
     8821 ataattcctt gcatttgtat attgcttatg ccccaatggc ggctgtttgc tttaatttaa
     8881 cgaggtaatt aattggggcg agagtggaga gcgctgggat tagtcaaccc tttgcttgag
     8941 ctttagaggc ggccacgcat attcagcgcc ttaggcaccc tcgccacaag ttcaactgcg
     9001 gtttttaggt taaaaattat tgatgcttat gggcctagtc gctatttggt gggtgcccag
     9061 tagcagctca ttgaaagctg tgtcgctagc aggcatccgc cggttacatc tgaatgcgtc
     9121 aatgtttgca attaccgaat taaaacttcg gcacgggtcg cccgccaggg gcaatttcat
     9181 aatgctcgtg ctcccgagca ttagcaaaga gttttcttat aagtcttaat cgtgtgggtg
     9241 gcagggcggt gtgtggcgat ccttttgtag gataattatc acctgcctat taaagatggc
     9301 ctgtattggg gctttgacgc acaaggatag ttggtaagcc cctgatttag gtgaaatgtt
     9361 cacacgtact tgatatatcc gtgtggctta aatcgcaggt ctactaactg cgccggtttt
     9421 tgagcgtctt actaggctgt tgccactggg tattttaagc cgagtatcct tagcatttct
     9481 gtgcagacct aaggtgtgga tcagggcagc gttggctgct tagttataag tattatgttt
     9541 gctataacca gggagccaat aaacacgggt ttagcacatt aaatgccatg cgattaattt
     9601 cctccagcct ggcagttaag ttgataactt tggtctatgc tgacgttaat aagctgcctc
     9661 aggcgataga tgcttagttg atttaaaccg gttatagttg ccgccctgca ggataggctg
     9721 ggagaagagg gcagctgggc tggcagtgcc gctaggttgg ctgcttataa tttcgggaca
     9781 agagtgaatg tattgggtca aggcggctta cgtgccggca ctgatctggt ttataagggg
     9841 ctacccgccc cctgagcgtt tggttaaata tctgttggag gactaatcta tgtcaataaa
     9901 ctcatcactt tcttcggacg gacgcacact gacaattgcg gttgatggcc ggtttgatgc
     9961 aggttcattg gatgaattcc gttcgtgtta tgaaagtgcc gtgcgcgatg ggccgatccg
    10021 ttttaatgtg gatttaaaac gggctgagta tttggatagc tctgcgctgg gaatgttgtt
    10081 ggtattgcgc gattatgccg gtggcgaaag tgcgtcgatt acgatttcca attgctcccc
    10141 tgaagttaag aaaatttttg ctatttcgag ttttgaaaag ttgtttacga ttcaataaat
    10201 aggcgatgta tcgtccgatt tagctcggtg ggattagcgc ggggttacag tgagtaaaaa
    10261 aattcctcaa tcgccgataa aggttttaat tgctgacgac tcgccggtcg atcgttttat
    10321 ccttcaaaca atgttggagc gcctcggtta taccgtggtt ctggcggagg atggccagca
    10381 ggcgattgag cagtttttag ttgaaaagcc cgatttagtt tttttagatg tattgatgcc
    10441 caatgtggac ggggtggaag cggcatcaag tattaaggaa gctgcagggg aggattttat
    10501 ccctgtgatt tttttaacat ccctggtgga tgccccttct ttagcgcgct gtctggatgc
    10561 cggtggcgac gattttttat ccaagcctta caaccagatt atcatcgaag ccaaaattaa
    10621 ggcttttagc cgcatgcggg tgatgaacag aaccctcagc ctgcaaaaaa atctcatcga
    10681 acgcaacaat aaacatctgt tacaagagca gacagtagcc aaacaagtat tcgatagaat
    10741 cgccaatact tcctcgctta atctttccat tattcgttat tacatgtcgc ctatggcggt
    10801 gtttaatggc gatgtgttgt tagcggatat cagccctacg ggcagtttaa tggtattgct
    10861 gggtgatttt accggccacg ggttacccgc tgccattggc tccatgccgc ttgcgtctat
    10921 tttttatggc atggtgaata aaggttttgg catgtcagag atcttgcggg aaattaatgg
    10981 caagctcaat aaaacgctgc cgatggggct attttgttgc gccacctttt tagatattaa
    11041 tttgcgtaaa aagcgcttgt tggtgtggag cggcggtttg cccgatggag caatttatcg
    11101 cgctggtggc aaaacagttg agcgcttggc ttcgcgcaat ttgcccctgg cgattttgtc
    11161 caaccagagt tttaaagcgg aagcggtgcg ttatgtttta gagccggggg atagggtttt
    11221 actctggtcg gatggcattc aagaggcgcg caatacagaa ggcgttatgt tcggtgagga
    11281 gcggctcaat gctcttttgg agcaggaact cgataatatt catatattcg acgacatact
    11341 gcaaaaagtg caacaccata taggggaggg cgaacgggac gatgatgtgt cgctggttga
    11401 agtgcgcttc cccgatgaaa aagaagccgc agaggtggaa aaacaaggtt ctcgtggccg
    11461 cggctgccta caggattggt ctttagattt tcgcttggaa gccagctctt tacgtgtctt
    11521 tgaccccatg cctttgctgc tgcatataac ctctgaagtg ccgggtttgc ggcagagtaa
    11581 ttcggtgtta tttactgtaa tttctgagct ttacaataat gtcgttgaac atggtgtgtt
    11641 ggggttaaaa tccagtatta aacaatcgcc ccagggcttt gcggaatatt atcgcctgcg
    11701 caaagagggt ttggagaatt tacccaaaga ctgttttgtg attttagaat ttagccacaa
    11761 ggttgatcct ggtggtggtg tgttgcgaat tcgggtgcga gatagcggtg cgggcttttg
    11821 cccagatgaa accgtggcga cacccggcgg tgtgccgatt tatcacggca ggggtttcgc
    11881 gttgttgcgt agcatgtgtg aatccatcac cgtgttgccg ccgggcaatt gtgtagaagc
    11941 tcaatttcga tggctggcag aggattaata gtatggcggc tgatgcgtcc ttaaatgcgg
    12001 aaatgctcga tgaattacgc gaactcttgg cggaaaattt taatgaatta atcaaccgct
    12061 atattgccga ttcccaaaca cgttttaata ccctgcacac cgccattgcc caagcggact
    12121 ttaaagtaat ttattacgag gcccatggca tcaaaggcag cagccgcaac atgggggcca
    12181 atattttggc tgagattatg gcgcggcttg aagcccttgg gcagagccaa aacccacagg
    12241 gattagcaga ggtttttgct gatgcacagg cggagtttgc aaaggttgtt gtggggttag
    12301 aggccttccg cacagccgaa tagttctccg cccctagtta tttcgacaga ccgctaaatt
    12361 aaactgtgta gccaccggcg gggcttgctt ggtaggtcgg ccattgcagc ggctgtcttt
    12421 tttatccttt gagccttgcg cttgggcgtg tagaattctg gccgaatccg tcctgttgag
    12481 aaagcccaat gcccactatc gttcgccaag acgacatcat cgcaagcgtt gccgatgcgt
    12541 tgcaatttat ctcctactat catcctatcg attttattca agcccttcat caagcctatt
    12601 tgcgcgaaga aaaccccgcc gcaaaagatg ccatggcgca aattctgatt aattcccgca
    12661 tgtgtgccca gggccatcgg cctatttgcc aggataccgg catagttaca gttttcgttg
    12721 aaatgggcat gaatgtgctg gttgaaggca ctatgagctt tgatgacatt attaacgaag
    12781 gcgtacgccg tgcttatatg cagccggata atattctgcg tgcgtctatt cttgccgacc
    12841 cggatggagc cagaaaaaat accggcgata acacacctgc cgtcattcac tacaggcttg
    12901 tacctggcga tactattgat attcatgtgg cggccaaagg cggtggttcg gaagcgaaga
    12961 ccaaattcgc catgctcaac cccagtgatt cggtggtgga ctgggtgtta tccgtggtgc
    13021 cgcagatggg ggccggctgg tgtccaccgg gtatgctggg tatcggcata ggtggcacag
    13081 ctgagaaagc catgttgctg gccaaagagg ctttgttgga gcctgtggat attcaggagc
    13141 tgcgcgagcg cggggcagct aatcgctcgg agcaattgcg cttggaatta ttcgataagg
    13201 tcaacgcgct gggcataggc gctcagggtc tcggtgggtt gaccactgt
//
LOCUS       DAOQGS010000038        13166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2189_length_13166_cov_4.560445, whole genome shotgun sequence.
ACCESSION   DAOQGS010000038 DAOQGS010000000
VERSION     DAOQGS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13166
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_13166_cov_4.560445"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PKZ52_02530"
     CDS             complement(<1..1251)
                     /locus_tag="PKZ52_02530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNG58672.1"
                     /translation="MSAQVHGAVKEKSTNTKTNVSASANTNTKTNKSTANNKKSSQPT
                     AKNTNKSAVRPTKSNKPTPVKPAAAKAVSTKDDIAWAQGVGAINKFKPDGSKPYYKQF
                     EDYVREVIAPRVPGAALTMVADGQVQVLEGFGTRAVGSTLPVNADTVFRLASVSKTFA
                     SGAAAVAVRDKLLSWDTTVQSKVPYVEFSNPNYGKQLTLKDILAQRSGLPKHTYTHLI
                     EENVPYPETLERLKYVNFACPPRKCYSYQNVVYSLSGDMIKAVSGKSYETYTTEKLFR
                     PLNMQTASFGLNAFDASPNKAMPHVWRSKGWSPTGSVDHNYYSVGPAAGVNASIRDMS
                     QWLLAQLGHKQDVLPVSLLNDMQQKITPNTLGDSHYADRNDLTNTHYGLGWRIFDFDG
                     QKNFAHHGGWVQGYRAEVVFNRQLQ"
     gene            1808..>3176
                     /locus_tag="PKZ52_02535"
     CDS             1808..>3176
                     /locus_tag="PKZ52_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNG58673.1"
                     /translation="MGRVWLLVCITFLLAACGEGKSSRVDQSKAALTPEQAAAREAQN
                     RQQAGQGLNISNPQKPTGNELGVSAGVPLFSDANLIVDVDGQVKGVAERVLWQQVSGP
                     KAIILNPQQAHTGIIAPDVEQVSELGFRLAAANGALVAVDELVLTVRPLSNPAKVVSV
                     EQEESLESLLFTIKLNKPAQTATAVRCITFDGTALAGSDYVAVDKLITFAPGEQEQTL
                     RVALIKDSVREQNEFFNLQVSGGESAEDAFSARAVGVIRETSSAPVVITEKPTPTPTD
                     IAQNQLKGTTGFVRVHIAWADAASVVALTVQDPCAHWLSAVNTASNCQDKLGRFEVFE
                     GVPEANIVWAQAPVGQYSVRLKHSSGTGSAYTVHVFWGNNSRVLKGVIGSGDTDTIFQ
                     FEITADELKNTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSS"
     assembly_gap    3177..3276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3277..5930
                     /locus_tag="PKZ52_02540"
     CDS             <3277..5930
                     /locus_tag="PKZ52_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HNG58674.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSGTPLPENFVGGEHVQITSNLTAKENYSLAMAHDL
                     AFDPKAFAGFTPANNEARGEFSITLDGVYQIDGFALWNDINANGEGVKNYSLRFYDEF
                     NNLLTERTGAGTPGDPNSLNDKGFTLQQFDGLIDSVAKIEFIVLDSFTQIEIRELGFT
                     GKKIRGVNFPASFIGYPHIRPATNLSVVEPYLLNQLADGILYETSANPAVVFNGFTSN
                     ESEGDIELYLDKAYTLSEFVLWNDINVQAEGISKFKLLFWDANGEPLGETASTNVSEN
                     QRNEGRFGFRPVSGVSRVTLQVLASHNISGYHRIEIRELGFIGVADKPDITITNETRS
                     AQGITITGQVDWGQGNRYQRVQAQLGGEPLNLFDHGLQFVEAQGAPWDKALARAQQLL
                     GNNWHPATITSAAELNNVSSLLDANQRYGDVYLGAYLDRGDNIQPYSAHWINGEPWGY
                     TAWGNSDGVPEPNDTGNNHYLTLRRGSSGWAWNDLNLQFDLGNVQGILMEQSAPSVTL
                     TDTAATTVFQLFIPNSYIHSDSLSLAFTDSAGRTNKIAYKLKNSSASTGVVLDAGTDF
                     FSRDNFALRGSLNGTVTGLKLRVNNSALPMSEYKIINQPLVSIDGVVPPEGWHLATIT
                     SAAEHQYLIRLLNASIQANQVVIGSSDQYYIGGRRNDGLWYWNTQELFNYQGWAPGEP
                     SGDGPALVLSGRQNWLWNDLPQLDANSQGYILERNAAAASIVEDAFDLVIPGGSLTAG
                     LNQVWLETVNAAGIPVNEPIYLTYAPD"
     gene            6529..7497
                     /locus_tag="PKZ52_02545"
     CDS             6529..7497
                     /locus_tag="PKZ52_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG58675.1"
                     /translation="MLRVSQLKRAYGDFIAAQGVSFTITKGEIVGLLGHNGAGKTTVM
                     KMLSGCLEPSGGEILFENRPLSLHLKAAQQKIGYLPESLPVYPELSVVDYLDYAADLK
                     GIDPAQKQREIKRVIEACELNDKLLSPISTLSRGYKQRVGVAQALLGKPSLLILDEPT
                     NGLDPQQTLHMRELIRALAQDATVILSTHIMQEVDALCDRVLMMRGGKLVIDAKLATL
                     RQSRYVRIRSSAPAPTIQTILAALPDVQTVELLNQDATPGSIRLEIKAGANCEAVCAA
                     AARSIVSQGADLFEVRAETRDLETLFREVNSQTKLDPVQEALNHAA"
     gene            7487..10366
                     /locus_tag="PKZ52_02550"
     CDS             7487..10366
                     /locus_tag="PKZ52_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNG58676.1"
                     /translation="MQPKTTLRRIAQKEITLFFSSPIAYLFLAAFAAVTLFTFFWGKA
                     FFARNIADVRPLFEWMPLLLIFLASTLTMRMWSEERRSGTLEHVLTQPVPLWHFVVGK
                     FLGCMALLAIALLITLPLPITVAILGDLDWGPVITGYVATLLLGAAYLSIGLCVSARS
                     ENQIVSLISAVAVCGFFYLLGSKTLTDFFGIQVAEWLRVLSTGARFDAITRGVLDLRD
                     LYYYLSLIAVFLALNTFALERERWAATGNQHHHRAWQSITALVLANALGANLWLGQIH
                     SLRLDATEGKQFSISPATEHYLSQLQEPLLMRGYFSGKTHPLLSPLVPQIRDLMKEYA
                     VKSNGKVRVEFVDPASNPELEEEANQKYAINPVPFQVADRYQAAVVNSYFNVLVQYGD
                     EYQVLSFRDLIEVKTASEPNIEVQLRNPEHDLTRAIKKVLHSYQAGGNLFDTVKGELV
                     FNAYISPEAKLPKELQAFKNLVQTHLKKQQEKSANRLQVNYLDPEADGGKLGEKIGKE
                     FGFKPMATNLLSSERFYFYLTLSQGSQVVSIPLEDLSEASFERNLNAAIKRFASGFTK
                     KVALVTPEPEFNNPHGAFGGPQPGAQFNQLEQFLGSELNIIHEDLKDGSVNGEADVLL
                     VTAPKNLSDTQLFAIDQFLMQGGTVILASAPYSANVSQRSISLDKTRSGLESWLEQKG
                     IKLDEHLIMDKQSVPFPIPVQRSVGGFSVQEMRMMDYPYFLDLRGEGLNSSHPITNGL
                     PQLTMAWASPIILDAQKQTGLNITPLMHSSSQAWLGDGTKVMPELNGNLLSGFTPSGE
                     QKSHLVGVIAQGKFTSWFAGKNSPLLQSAAGTPAAGATNGATLNRVIEQSPDSARIIL
                     FSSNDFLTDSILSMAGVANGGQYLNSLQMLANSVDYAVEDDGLASIRSRGNFNRTLPA
                     MEESSQAFWEYLNYGLALAALLAVGLIQRYIKRAREHHYQVWMAH"
     gene            10379..11395
                     /locus_tag="PKZ52_02555"
     CDS             10379..11395
                     /locus_tag="PKZ52_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNG58677.1"
                     /translation="MNQSVFVPNQLTQVKMTSALGQWQKRLGGLLAAQLILAGAIFAW
                     QEARQPDVHSEPLMAVKQNEVDKLVITEGQNSVTLQKVNGQWQLPQVQQLPVDEQKLT
                     GLLDKLANTQLTWPVATTGSSHERFEVSEQKFQRKINAYKADQSVVEFYLGTSPSFKK
                     VHLRKQGDKTVYAVGLSAFDFYSTAPNWLKKDLLALADVQTINGSDFSLRKTANGWSL
                     EGSNDSVDKNKADDLAAAFAKLAVLEPADNLPNGEPRNLTVTAAGQPYQFTLVKADNQ
                     YFIRRGDKPQVFKISQADYDRLTKPVKNDLLAKPTTTGANLDPVGQMLQQSTEGIFKA
                     KPAQ"
     gene            12078..>13166
                     /gene="nifB"
                     /locus_tag="PKZ52_02560"
     CDS             12078..>13166
                     /gene="nifB"
                     /locus_tag="PKZ52_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNG58678.1"
                     /translation="MQLTVLGQDESSAPAASSCASGSCGSSNDQMTALPDAIREKVQN
                     HPCYSEEAHHYFARMHVAVAPACNIQCHYCNRKYDCSNESRPGVVSEVLTPEQAVKKT
                     KAVAAAIPQMTVLGIAGPGDPLANPTRTFETFRMLSEQAPDIKLCVSTNGLALPDSVE
                     ELAKHNIDHVTITINCVDPEVGAKIYPWIYWNNKRIHGVKAAKILIEQQQKGLEMLVA
                     KGILVKVNSVMIPGVNDEHLKEVSRIVKQKGAFLHNVMPLIAEAEHGTFYGIMGQRGP
                     EPEELQDLQDSCAGDMNMMRHCRQCRADAVGLLGEDRGEEFTMDKIEVMDVDYEAAME
                     KRAVIHAGILEELNEKQAKRERLAAVNMP"
BASE COUNT         3250 a         2977 c         3308 g         3531 t
ORIGIN      
        1 ttgcaattga cgattaaaca caacttcggc tcgataaccc tgtacccagc cgccgtggtg
       61 ggcgaagttt ttctggccat caaaatcaaa aatccgccag cctaggccgt aatgagtgtt
      121 ggttaaatcg tttcgatccg catagtgaga atcacccaat gtattggggg taattttttg
      181 ctgcatatca ttgagtagac ttaccggcaa aacatcttgt ttgtgaccca attgggccag
      241 cagccattgg gacatatcgc ggatagaggc atttacaccg gcggcgggcc ccaccgaata
      301 ataattgtga tcgacgctgc cagtgggtga ccagcctttg ctgcgccata catgtggcat
      361 ggctttattg ggcgaggcat caaaggcgtt caaaccgaat gaagcggttt gcatatttag
      421 cgggcggaat aatttctccg tggtgtaggt ctcatagctc ttgccgctga cggctttaat
      481 catatcgccg cttaagctgt acaccacgtt ttgatagcta taacacttgc gcggcgggca
      541 ggcgaagttc acgtatttta ggcgttctaa agtttcgggg tagggaacat tttcttcaat
      601 taaatgggtg taggtgtgtt tgggcagccc cgagcgctgg gccaaaatat ctttaagggt
      661 taattgcttg ccgtagtttg gattgctgaa ttccacatag ggcactttgc tttgtacggt
      721 ggtatcccag ctgagtaatt tatcccgtac ggcgacagcc gcagcgccgg aggcaaatgt
      781 tttggaaact gaggccaatc gaaacaccgt atcggcattt accggtagtg tgctgcccac
      841 ggcgcgggtg ccaaagcctt ctaatacttg tacctggccg tccgctacca tggtgagggc
      901 tgcgccgggt acgcgagggg caatgacttc gcgcacatag tcttcaaatt gtttgtaata
      961 gggttttgaa ccatcgggtt tgaatttgtt aattgcaccc acgccctggg cccaagcaat
     1021 atcatcttta gtggaaaccg ctttggccgc tgcaggtttt acaggtgttg gtttgttgct
     1081 tttagtcggg cgaacagcgg atttattggt atttttggcg gtgggttggc tggatttttt
     1141 gttatttgcg gtgcttttat tcgttttggt attcgtattg gcgctcgccg atacattggt
     1201 ctttgtgttg gtggattttt cttttacggc cccatggacc tgtgccgaca cgcctgtaag
     1261 gctcaggagc gtgaaaataa aaaaaggcga aaatttatgc ttaatcataa tcgtgtgagg
     1321 gataccacag tggcgctaat cgggatgttg ttattggtcg aatgacccgt gccgctaagt
     1381 ttagccgatt ttttggcgcc aggcgtctta ttcatgtgct ggcaaataga ttgtcctgaa
     1441 gtttgccttt ggcccaccca aagggtcaaa aatgtgtgga cttttgtctt tggcgggggc
     1501 gctatagcaa agttgccgat ctggcagccc tgttgccgtt atttgccgtc ctcaatagcg
     1561 ctttatatcc cggggtttcc caaacgggtt gcagcaagga ggaagggaat ttttaccaag
     1621 aactgcgcat attcggcgtc ttaagcaccc tcgctacggg gccagctgcg ttttttgggt
     1681 ttaataaaaa tagcgaaaaa taagcttttt tagattcttc ggcgtcatct gctgaagatt
     1741 agggaggtgt catagattta ttagggtaaa caattaaaat gccccgcact atttttaagg
     1801 gggctctatg ggacgcgttt ggctgttggt ttgtattact tttttacttg ccgcttgtgg
     1861 tgaaggcaag tcatcccggg tggatcagtc gaaggcagcc cttacccctg agcaggccgc
     1921 cgcccgcgag gcgcaaaacc gccaacaggc cgggcagggg ctcaatatca gcaaccccca
     1981 aaaacccact ggcaatgaac tgggcgttag cgctggagtg cctttgttca gcgatgccaa
     2041 tttaatcgtt gatgttgatg gccaggtaaa gggcgttgcc gagcgggttt tatggcaaca
     2101 ggtgagcggc cccaaggcga ttatcctcaa tccgcaacaa gcccacaccg gcattattgc
     2161 gccggatgtg gagcaggtaa gtgaactggg ctttaggctt gcggcagcaa atggtgcgct
     2221 agtggctgtt gatgaactag tgttgacggt gcgcccctta agcaacccgg ccaaggtagt
     2281 aagtgttgag caggaggaaa gccttgaaag cctgctgttt acgatcaagc tgaataaacc
     2341 tgcgcaaact gcaactgcag tgcgctgcat tacttttgat ggcactgcct tagcaggttc
     2401 tgattatgtg gcggtggata aattaattac ctttgccccg ggggagcaag aacaaaccct
     2461 gcgtgtggca ttaattaaag actctgtccg cgagcaaaat gaatttttta atctgcaagt
     2521 aagtggcggc gaatcggcag aggatgcgtt tagtgcgcgg gcggttgggg tgattcgcga
     2581 aacatcttca gcaccagtgg tcatcactga aaaacctaca cccactccaa ctgatattgc
     2641 acaaaaccag ttaaagggta cgacaggttt tgtgcgcgta catattgcct gggcggatgc
     2701 cgccagtgta gtcgctctga cggtgcaaga cccctgtgca cattggttga gcgcggtaaa
     2761 taccgcgagt aattgccaag ataaattagg ccgttttgaa gtatttgaag gtgtgccaga
     2821 ggccaatatt gtgtgggccc aggcaccggt tggtcaatat agtgtgcgcc taaaacacag
     2881 cagcggaaca ggtagcgctt acaccgtaca tgttttttgg ggcaataata gccgcgtgtt
     2941 aaaaggtgta attggcagtg gcgatacaga cactattttt cagtttgaaa ttactgccga
     3001 cgaattaaag aacacatcga gttcttccag tagttctagc tcttcaagta gctctagttc
     3061 ttcaagcagt tctagttctt caagcagttc tagttcttca agcagttcta gttcttcaag
     3121 tagttctagt tcttcaagta gttctagttc ttcaagtagt tctagttctt caagtannnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctag ttcttcaagt agttctagtt
     3301 cttcaagtag ttctagttct tcaagtagtt caagctcttc aagcagttcc agttcttcaa
     3361 gtagttctag ttcttcaagc agctctagtt cttcgagcag ttccagttct tccagcagtt
     3421 ccagttcttc cagcagttcc agttcttcaa gtagttctag ttcttcaagc agttccagtt
     3481 cttcaagtag ttccagttct tcaagcagtt ctagttcttc aagcagttct agttcttcaa
     3541 gtagttctag ttcttcaagt agctctagtt cttcaagcag ttctagctct tcaagtagtt
     3601 ctagctcttc aagtagttcc agctcttcaa gcagttctag ctcttcaagc agctctggaa
     3661 cacctctgcc ggaaaatttt gtgggcggcg agcatgtgca gattacgtct aatctgacag
     3721 cgaaagagaa ttattcattg gccatggccc atgatctggc ttttgaccct aaggcatttg
     3781 ctggttttac acctgctaat aacgaagcgc gcggggaatt tagtataacg ctagacggtg
     3841 tgtatcagat tgatggattt gcgttgtgga atgatattaa tgccaatggc gagggcgtga
     3901 agaattatag tctgcgtttt tacgatgaat ttaataatct gcttaccgag cgaaccggtg
     3961 ccggaacccc tggcgaccct aacagcctaa acgacaaggg ctttaccttg cagcagtttg
     4021 acggcctaat tgattcagta gccaaaattg aatttattgt gctggactcg ttcacgcaaa
     4081 ttgaaatacg cgagctgggc tttactggca aaaaaatacg gggcgttaat ttccctgcct
     4141 cgttcatcgg ttatccgcat atccgcccgg ccacaaactt atcggtggta gaaccctatt
     4201 tattaaacca gctggcggac ggaattctct atgaaaccag tgcaaacccc gctgtagtgt
     4261 ttaatggctt taccagtaat gagtctgaag gcgatataga gttatatctg gacaaagcct
     4321 atacgctcag tgaatttgta ttgtggaatg atatcaatgt gcaagcagag ggtatttcta
     4381 aatttaaatt attgttttgg gatgccaatg gagagcccct gggagaaacc gcctcaacta
     4441 atgtgagcga aaatcagcgc aatgaaggca gatttggttt ccgccctgtg tcgggtgtaa
     4501 gccgggtgac tttacaggtg ctggcttcac acaatattag cggctatcac cgcattgaaa
     4561 ttcgcgagtt gggttttatt ggtgttgccg ataaaccgga tattactatt accaatgaaa
     4621 cccgcagcgc tcaagggatt acgattactg gccaggtgga ttgggggcaa ggcaatcgct
     4681 atcagcgggt gcaggcacaa ttaggcggcg agccattaaa cctatttgac cacggcttgc
     4741 aatttgttga agcccagggc gccccttggg ataaggcgtt agcccgcgcc cagcaattac
     4801 tgggcaataa ttggcatccc gccactatta catctgcggc tgagcttaat aatgtgtctt
     4861 cactgttaga tgccaatcag cgttatggtg atgtttattt aggggcatac cttgatcgcg
     4921 gcgacaatat tcagccctac tccgcccact ggattaacgg tgagccctgg ggttacaccg
     4981 cctgggggaa tagtgatggc gtccccgagc ccaatgacac cggcaataat cactatttaa
     5041 ccctgcgccg aggttcatcc ggttgggcgt ggaatgattt gaatctgcaa tttgatttag
     5101 gcaatgtgca aggtattttg atggagcaat cagcgccctc ggtcaccttg accgacacgg
     5161 cagcgaccac agtatttcaa ttatttatcc ccaatagcta tattcacagt gatagtttga
     5221 gcctcgcgtt taccgatagc gctggccgca ccaataaaat tgcttataaa ttaaaaaata
     5281 gcagcgcttc tactggagtt gtgttggatg ccggtaccga tttttttagc cgagataatt
     5341 ttgcgctgcg aggcagttta aatggcactg taaccggctt aaagttgaga gtgaataata
     5401 gtgccctgcc catgagtgaa tataaaatta ttaaccaacc gctggtttct attgatgggg
     5461 ttgtgccgcc agaggggtgg catttggcaa caattaccag tgccgctgaa caccaatatt
     5521 taattcggct attaaatgcg agtattcagg ccaaccaagt cgtaattggc agcagtgatc
     5581 aatactacat aggcggcaga agaaatgacg gcctatggta ttggaataca caagaactgt
     5641 ttaattacca aggttgggcg cctggtgaac ccagtggcga cggcccggct ttggtattaa
     5701 gtggtcggca aaattggctt tggaacgact tacctcagtt agatgcaaat tcccaaggtt
     5761 atattctgga acgcaatgca gctgcggcgt cgattgttga ggacgcattt gacctggtaa
     5821 ttcccggcgg aagtttaacc gcaggcttaa atcaagtctg gttggaaacg gtgaatgctg
     5881 cgggcatccc ggttaacgaa cctatttatc tcacctacgc acctgactaa cagcttgttg
     5941 aaatactgcc ttgccagcgc gtcgccgcct aactatttca acaagctgct aatgttattg
     6001 ggtttgccag gccgttaagc atttgccatt ttattggggc aatcccatac gcaaggggcg
     6061 ccacctgttc gttgttgcca attactggcg ttgcggcgat tgctttggca cctggggagc
     6121 tgagtttggt ggcgcgatga ctgggtgtaa attccccttc gccacctctt gaactttacg
     6181 cacctaaccc tatctatagc cccggtttta ctttggccgc tgtttggcga ggggctcgtt
     6241 agagctttta tggcgcgcgg ccattggcag ttctacctta tttaattcac tttgattgtc
     6301 attacgattg ttttgtgggg cttctcccgt caacgaggaa aaaacaatgt ataacgcgat
     6361 cactcgctca cacttattgc ctagattacg cctgtttttc catctcggca tcccgcccgg
     6421 ttaaattcca catgtaattg atttcctttt tggtgtcttt tagagccaaa ggattttatt
     6481 cgccattcac ttttcaaata attttaatcc acacgatttg gaggctatat gctgcgcgtg
     6541 tcgcaactta agcgtgccta tggtgatttt atcgccgccc aaggggtgag ctttactatt
     6601 accaagggtg aaatagtcgg tttgctcggc cacaacggtg ccggtaaaac cacggtgatg
     6661 aaaatgctca gtggttgttt ggaacccagc ggcggcgaga ttttatttga aaaccgccca
     6721 ctctccctgc atttaaaagc cgcgcaacaa aaaataggct atttgccaga gagcctgccg
     6781 gtgtacccgg aattatcggt ggtggattat ttagattatg ccgctgactt aaaaggtata
     6841 gatcctgccc agaagcagcg ggaaattaag cgggttattg aggcgtgtga attaaacgat
     6901 aagctcttaa gccctattag caccctatcg cgtggctata aacagcgggt tggagtcgct
     6961 caagcccttt taggcaaacc cagcctcctt atattggatg agccaaccaa tggtttagat
     7021 ccacaacaaa cgctgcatat gcgtgaatta attcgcgccc tcgcacaaga cgccacggtt
     7081 attctctcca cccacattat gcaagaagtg gatgcgctct gtgatcgcgt gctgatgatg
     7141 cggggcggta aattagtgat cgatgccaaa ttggcaactt tacggcaaag ccgctatgtg
     7201 cgtatccgca gtagtgcacc tgcacccaca atacaaacca ttttggctgc actacctgat
     7261 gtgcaaacgg tggagctatt aaatcaagac gccacacccg gcagtatccg cttggaaatt
     7321 aaagccggtg cgaattgtga agcggtgtgc gccgccgccg caagatcgat agtgtcccag
     7381 ggcgctgacc tatttgaggt gcgagctgaa acccgcgatt tagagacatt atttagagaa
     7441 gtcaactcgc aaacaaaact agaccctgtt caggaggcgt taaaccatgc agcctaaaac
     7501 aactctgcgg cgcattgccc aaaaagaaat taccttgttt ttttcatcgc cgattgcgta
     7561 tttatttttg gcggcttttg cggctgtaac cctgtttaca tttttttggg gtaaagcctt
     7621 ttttgcccgc aatattgccg atgtgcggcc cttatttgag tggatgccac tgttgctgat
     7681 ttttttggcg agcacactca ctatgcgcat gtggagtgaa gagcgccgca gcggcacatt
     7741 agaacatgta ttaacacaac ctgtgcccct gtggcatttt gtggtgggaa aatttttagg
     7801 ttgcatggcc ctgcttgcaa ttgcactgct tatcacactg cctttgccca taacggtagc
     7861 cattttagga gatcttgatt gggggccggt tatcaccggt tatgtggcaa ccctattgtt
     7921 gggcgccgct tatttaagta tcggcttatg tgtgtctgcc cgcagcgaga atcaaattgt
     7981 tagtttgatt agtgctgtag cggtgtgtgg ctttttttat ttgctcggca gcaaaactct
     8041 taccgatttt tttggcatcc aagtggctga gtggctgcgc gtgttaagta cgggcgcgcg
     8101 ctttgatgct attacacggg gtgtgttgga tctgcgcgat ctttattatt acttaagttt
     8161 aatcgcggta tttctcgctt taaatacctt tgcgctagag cgtgagcgct gggcggctac
     8221 gggcaatcaa catcatcacc gagcttggca gagtattacc gctttggttt tggccaacgc
     8281 gttgggtgct aacctctggt tagggcagat tcacagcctg cgtttagacg caactgaagg
     8341 caaacagttt tctatctccc ccgccactga acattatctt agccaattgc aggaaccctt
     8401 gctgatgcgc ggctatttta gtggcaaaac acatccgctg ttatcgccct tggtgcccca
     8461 aattcgcgac cttatgaaag agtacgcagt aaaaagtaat ggcaaggtga gggtagaatt
     8521 tgttgaccct gccagcaatc ctgaattgga ggaggaagcc aatcaaaaat acgctattaa
     8581 cccagtgccc ttccaagtgg cggatcgcta ccaagccgca gtggtgaact cctattttaa
     8641 cgtgttggtg cagtacggtg atgaatatca ggtgctcagc tttcgcgatt taattgaagt
     8701 aaaaaccgct agtgagccca atatagaagt gcaactgcgc aatcccgaac acgatttaac
     8761 ccgcgcgatt aaaaaagtgc tgcatagcta tcaagccggt ggcaatttgt ttgacacagt
     8821 aaaaggtgag ttggtattta atgcttatat ttcacctgag gctaagttac ccaaagagtt
     8881 acaggccttt aaaaacctag tgcaaaccca tttgaaaaag cagcaggaaa aatccgccaa
     8941 ccgcttgcag gtgaattatt tggacccgga agccgatggc ggcaagttgg gtgagaaaat
     9001 tggcaaagaa tttggcttta agcctatggc caccaattta ttaagcagtg agcgattcta
     9061 cttttatctc accctttccc agggcagcca agttgtctct attccgctag aagatttaag
     9121 cgaggcgagc tttgagcgca accttaatgc ggcgataaaa cgctttgcca gcggctttac
     9181 taaaaaagtt gccctggtaa caccggagcc tgaatttaat aatccccacg gtgcatttgg
     9241 cggcccgcag cctggtgccc agtttaatca gcttgaacag tttttaggta gtgagctgaa
     9301 tattatccat gaggatctaa aagacggctc agtgaatggt gaggcggatg tattgctggt
     9361 aacagcgcct aagaatctta gtgataccca gttgtttgcc attgaccaat ttttgatgca
     9421 aggcggtaca gtgattctcg ccagtgcgcc ctattcagcc aatgtcagcc agcgcagcat
     9481 tagcctggat aaaacccgct ctgggctgga atcctggctg gagcaaaaag gtattaagct
     9541 ggatgagcat ttaattatgg ataaacaaag tgtgccgttt ccgattcctg tacagcgcag
     9601 cgtgggcggc tttagtgtgc aggaaatgcg tatgatggat tatccgtatt ttctcgacct
     9661 gcgcggtgag ggcttaaatt catcccaccc cattaccaat ggcttgccgc agctaaccat
     9721 ggcttgggca tcgccgatta ttttggatgc ccaaaagcaa acgggtttga atataacccc
     9781 gttgatgcat agttcgtccc aggcctggtt gggggacggc actaaggtga tgcccgaatt
     9841 aaacggcaat ttactcagtg gttttacgcc cagtggcgaa caaaaatctc atttggtggg
     9901 agtaattgct cagggtaaat tcacctcctg gtttgccggt aaaaattctc ccttgctgca
     9961 atcagccgct ggcacccctg ctgcgggcgc aacaaatggc gctaccttaa accgcgttat
    10021 tgagcaatcg cccgattccg cgcgcattat tttatttagc tccaatgact ttttaaccga
    10081 ctctatcctc tccatggccg gtgttgccaa tggcggccag tatttaaatt ccctgcaaat
    10141 gctggctaac agcgtggatt atgcggtgga agatgatggc ctcgccagta tccgcagccg
    10201 aggtaatttc aaccgcacct tgccggctat ggaggagagc agccaagcct tttgggaata
    10261 cctcaattat ggcttggcac tggctgcgtt gctcgccgtt ggcttaatcc agcgctatat
    10321 aaaacgcgcc cgcgaacacc actatcaagt ttggatggca cattaagagg tgaatcctat
    10381 gaatcaatca gtatttgttc ccaatcagct aacacaagta aaaatgacat cagccttagg
    10441 gcagtggcaa aagcgcctag gagggttgct ggcggcgcaa ttaatattgg caggcgctat
    10501 tttcgcatgg caagaggcta ggcagccgga tgttcatagt gagcctttaa tggcagtaaa
    10561 acaaaacgaa gtagataagt tggttattac ggaggggcaa aattctgtga ccctgcaaaa
    10621 ggtaaatggc cagtggcaat taccgcaggt gcaacagcta cccgtagatg agcaaaaatt
    10681 aacagggcta ctggataaat tggctaatac ccaattaacc tggccggtgg ctactactgg
    10741 cagcagccac gagcgttttg aggtgagtga gcaaaaattc caacggaaaa ttaacgccta
    10801 caaggccgat cagtcggtgg tggaatttta tttgggcacc tctccgagct ttaaaaaagt
    10861 ccatctgcgc aagcaggggg ataaaacagt ttacgccgtg ggtttaagtg cgtttgattt
    10921 ttacagcaca gcgcccaatt ggctgaaaaa agatttactt gctttggctg atgtgcaaac
    10981 catcaatggc agtgatttta gtttgagaaa aactgccaat ggctggagct tggaaggctc
    11041 aaatgacagt gtggataaaa ataaggccga cgacctagca gctgcgtttg caaagcttgc
    11101 ggtgcttgag ccagcggata acctgcccaa cggtgaaccg cgaaacctaa cggtgacagc
    11161 cgcaggccag ccctaccaat ttactttggt gaaggcggat aatcagtatt ttatccgtcg
    11221 cggcgataaa ccgcaggtat ttaaaattag ccaggcggat tatgatcgcc tgactaagcc
    11281 ggtaaaaaat gatttgctcg caaagcccac taccacaggt gcgaacctcg atccggtggg
    11341 gcagatgttg cagcagtcaa cggagggtat ttttaaagcc aagcctgcac aatagtgcct
    11401 gccgatattc gttttacgct tcgcaataat tttatggcct ctgtttagag gcctttttta
    11461 ttttggttgc tgttgttttg gattttgtaa ctattccgca ctgcggggag tactgtgggt
    11521 tcacaacact aaagcattcg cctggtgatc tctattcggc acacgaataa cgtccagcta
    11581 caaaaatggt gccgcccaca aaataatgct gctgaaaaaa tagtgcttaa aatagttgct
    11641 agatttttag atggctcatt gcccgagccc accgcgtttt accagactaa tttcctgtat
    11701 cggtctgcgc aattcccggt ggccacacat tgagatgagc cagcttttaa agtctatacc
    11761 tgtatccgtg gtgtaggtct gcaagaatta ccgatcaagc cttttaccgt ctttttgtaa
    11821 aaacccagtg gcctactggg ttttttgtgc ctgcaatctg tgtcgtgtat gccacaaggc
    11881 ctgcgtggct ttgttgtgtc aactgccaca aactccctct gcatcgaata aaaatcataa
    11941 taaaaacagc tggttaaaag ttgggcatag gcgttgcaac gcccagtgtg cagcagtaac
    12001 tgtaaacgca gaagccgcca ggctctgcct aacctttaaa tagtccgccc atagcggcgt
    12061 gaataggagt tcttgcaatg caactgacag tgcttggaca agatgaaagt agcgcacctg
    12121 cagccagcag ctgtgcttcc ggttcctgtg gcagcagcaa cgaccagatg acggccttgc
    12181 ctgatgctat tcgggagaaa gtgcaaaatc acccctgtta ttctgaagaa gctcaccact
    12241 atttcgcgcg tatgcacgtt gcggtggctc ctgcctgcaa tattcagtgc cattattgta
    12301 atcgcaaata cgattgctct aatgaatcgc gcccgggtgt ggtgtctgaa gtattaaccc
    12361 cagagcaagc ggttaaaaag accaaagccg tggccgccgc aatcccacaa atgaccgtgt
    12421 tgggaattgc tggccccggt gatccacttg ccaacccaac gcgcacattt gaaaccttcc
    12481 gtatgctctc tgagcaggcg ccggatatta aattgtgtgt ttccaccaac ggccttgccc
    12541 tgcctgattc agtggaagaa ttagccaagc acaatattga ccacgtcact atcaccatca
    12601 actgtgttga tcctgaagtg ggtgcgaaaa tttacccgtg gatttactgg aataacaagc
    12661 gcattcacgg cgtaaaagcc gccaaaattc tcattgagca acagcaaaaa ggtttggaaa
    12721 tgctggtggc gaaaggaatc ttggtaaaag tgaattccgt gatgattccc ggtgttaacg
    12781 atgagcattt aaaagaagtc agccgcatcg ttaaacaaaa aggtgcgttt ttacataacg
    12841 tcatgccgct gattgccgag gccgagcacg gtaccttcta cggcattatg ggccagcgcg
    12901 gccctgagcc ggaagaatta caggatctgc aggattcctg tgctggcgat atgaatatga
    12961 tgcgccattg ccgtcaatgc cgcgccgatg cagtaggttt gctcggtgag gaccgtggtg
    13021 aagaatttac catggataaa attgaagtga tggatgtgga ctatgaagcg gctatggaaa
    13081 aacgcgcagt tattcatgcc ggtattttgg aagaactcaa tgaaaaacaa gccaagcgcg
    13141 aacgtttggc ggccgtgaat atgcca
//
LOCUS       DAOQGS010000039        13064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2204_length_13064_cov_4.866016, whole genome shotgun sequence.
ACCESSION   DAOQGS010000039 DAOQGS010000000
VERSION     DAOQGS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13064
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_13064_cov_4.866016"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PKZ52_02565"
     CDS             <1..1436
                     /locus_tag="PKZ52_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNG58679.1"
                     /translation="GDPMTLVARGAALYAATIGFKNTRQADATAQAHTAKNACELWLQ
                     YPSVCADVQPDIMGRILDAKHFAPARVQLIRDDGQWESNPIELDENGVFFISVYLVAA
                     KNNKFLIKLLDKKGGVIPAMPDTIAIVHGLTISDPPLSRSIGVALANGQRRSFIDRGT
                     PLPAKRSFLQYSAETLVPGSGQQLNIPIVQGERAQARFCRKVGNLVIPAAQLQQQLPA
                     GSPIEITIEVDRGGNLKASALIVDQKKLITSVAEILTPSADPTALQVGFNQVQARLLQ
                     QQREAFARRDQAAISVLDQLAKKLHGVNKDLLLASGDQDAALRAQRNLMEVEAELETF
                     ESRDQLEELMRECEIVYLNTRHQVDQYGNEVEKKMLADCEVRFNKAIELKRVQEVERL
                     IEQMSNLFNSAYRRNPDFWIDQFHAVCSRLHEATHLAKANQLAAEGRRYLADSNLAAI
                     ESCVRQLWNLIPDERLSGEENHQSGIQ"
     gene            1489..1833
                     /locus_tag="PKZ52_02570"
     CDS             1489..1833
                     /locus_tag="PKZ52_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58680.1"
                     /translation="MSAEFASTPLFSAWPFWVLELDPAADNRSIERAFQKFTGSLQLN
                     IANAKNYMTPLGLRPRDEFLLREARAILTNPDQRALAEFWYMPAKVQAAAPTQQVDTK
                     PKLDWREILRTP"
     gene            2042..3400
                     /locus_tag="PKZ52_02575"
     CDS             2042..3400
                     /locus_tag="PKZ52_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58681.1"
                     /translation="MKDHTHPPTAPKDVWVWLTLFPVVILAYLSVFVLDQLSIPSLIA
                     LVVVFSIASLILFPSLFAQASIYLGWVKVSYWLGRIAYNRHQKDLFAGALFFGWRAAH
                     HAKGDKKSQGHEWLAQRLNNYRGTLGSGTMLVHAMMKAEPLAYKDLRAQFIWLQGINK
                     NWLPENIARAAFRFILARACREEDFPEIRAQARYWQLVCKNRFAQWVDMYHWSLGADV
                     IPRMLKLKITWLYFWVGNTQLRSFLPHLFKQSAAPTTSQMNLAQLKEAELNLFQSGAK
                     NSQWINDAWRSYSAGPMADEWLPRIKALGSFNENEVLSRLQQSVLDLISLRSGEGDLD
                     SEAAMAERDNGFKLLQIKIAAMVNRSEQNKMMTGIQEYEEFLALLRVAERLGKDDHSR
                     AQVFFHIRSPVWNWMVELWNNTKNRPLAFLAGNYMAPLAREFGDTDAYEFFSGIVGHK
                     FT"
     gene            complement(3596..3931)
                     /locus_tag="PKZ52_02580"
     CDS             complement(3596..3931)
                     /locus_tag="PKZ52_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNG58682.1"
                     /translation="MSLPPCPACKSEFTYQDGGLYICPECAHEWSDAPAEASDTRQVR
                     DAFGTVLNDGDSVTVIKDLKVKGSSLVIKVGTKVKGIRIVDGDHDIDCKIDGVGAMSL
                     KSEFVKKAN"
     gene            4016..5245
                     /locus_tag="PKZ52_02585"
     CDS             4016..5245
                     /locus_tag="PKZ52_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNG58683.1"
                     /translation="MLNHIWAGLILLALASAALQALTGADAQVLARMTQALIDCGKLG
                     FELAIGLTGMLCLWLGLLAIAEAAGLVALLSKGLRPLFVHLFPQVPKDHPAMGSMSLN
                     IAANMLGLDNAATPLGLRAMAQLQTLNPHPEQATSAQTLFLIINTASVTLLPVSVLTY
                     RAQLGAAQPADVFLPLLLTSLSATLIGVIVAGFCLRLNLRHPVVLAYGAGLFALIATV
                     TLSLNGASSEYMQQVSQYFSQGLLLITVAGFLLAGVVKKVPVYEVFINGAKEGFSTAV
                     TLIPYLVAMFLAISLLRASGSLDMALHGVKTCVNFFGFDARWVDALPTMILKSLSGSG
                     ARASMLDVMRVQGADSFAGRLVSIIQGSSETTFYVLTVYFGSVGIRRCGIALGCALVA
                     DVAAAVLGVIYCYGFFG"
     gene            complement(5542..6537)
                     /locus_tag="PKZ52_02590"
     CDS             complement(5542..6537)
                     /locus_tag="PKZ52_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNG58684.1"
                     /translation="MSHLSAFHSDIKTILEQARGKARSAVNAAMVEAYWLIGQRIVQE
                     EQQGKLKAEYGKRLIEDLSTALTSDFGKGFSYANLYNCRQFYLTFPKQDILYTVCREL
                     SWSHLRLIMRVESPQAIDYYCKEAREQNWTVRQLERNIKSQSFQRLLSTQSAQSSTSG
                     TSAHLEFIKDPYVLEFLQLPETGQLKENKLEQAIIDELQKFLLEMGKGFSFVARQMRI
                     STETSHFFIDLVFYNYLLKCFVIIDLKTGKLTHQDIGQMDMYVRMFDDLKRGEDDNPT
                     IGIILCDSKDETIVKYSVIKENQQLFASKYQRILPTEEELIAEIEREKRLIKGSV"
     gene            complement(7056..10769)
                     /locus_tag="PKZ52_02595"
     CDS             complement(7056..10769)
                     /locus_tag="PKZ52_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNG58685.1"
                     /translation="MKLIAEKYRTIKPTINYLNDPIIITQAWKKTHGYIRSFNWYADT
                     LALDISALGIEENAHKWAEQIKKGSLLHPIELVPAAKSEPWIVDEVKGWVPKSRDDKK
                     PPLRPLAHITIRDQTWASAVMLCLADAVETAQGDCSNTNFSFKSAQENKVYSYGNRLV
                     CDWQDERAWFRWGNSDTYRKFFTDYQNFIERPIAIGKQISESSADQENVYVVSFDLSK
                     FYNKIDRTVLIERLKALSREYGNDYDDMFWQAVARITNWQWRDDSLKYAKKIKEFSTE
                     LGLPQGLVASGFFANAYLVEFDRAIGENINRYISSNLSITLHDYCRYVDDLRLVIHSE
                     STDIAALSDELNIWIGKIFKKTGNKALSINPKKTKITKLSDLDNAGSISSRIKMIQSE
                     LSGPADRDILESSSGLLEGLLNIESDTKIDLDALKENKKSDIKLVQLTQFDHDIRPDT
                     LKRFAANRLESIMRSKRKIDAHSNENADADYFIKSDNESELLAKKLILAWMKDPSLGL
                     VLRKAIEIFPNPDMFEPVIEAIYSRSTYGKHANKITCILMDYLLADLFRCAADFHGYF
                     QTVSYPNSANPSGILELISRFAQKTTSSKSSKKFVARQALMLLGVLNKPILVERFEDS
                     IQQTLHAILAGSLPKRFQQETIALFEVASQITDNTDSYASILIEHIEKTGTDKINAII
                     PFAKRGGPFWLSLWKILDKREELLDVKEQLLWAKPSSFVKRSASKQRLAHIICSEENG
                     FEHEAALLKLAIGLVKLACNNPKAISASPNSIMIDKEKITSWIELWHKDIEVKCILVG
                     KSLSDPRFSIPSWIETVDGKPEKERAIIYWVGSILRASVLGGVDFTGNRWKKGKTITY
                     KGLRTSWYKRRMGMMHSPEKIIGEFATVTSWFSELLMVSLQWPGFQSSYVSNLDIGEI
                     ASLEDFLVCLKSRLKKLNDMYCRSSYLPLLPTRIDRPKNDKDNFRLVTVQQLLPKTND
                     FSLADPKLNNPITRAKHREHIMAICQLTYKTLEAKLATDTDKSKPAADLIVFPEVAIH
                     IDDQDIIKRLADKTQSMIFAGLVFTDHNGKLVNIARWFIPHYKEGARQWIIRDQGKQY
                     MTPNEKLLGVEGYRPCQHLIEVHGHPEGPFKLSGAICYDATDISLAADLRDKTDLFVV
                     AAHNSDVATFDSMAAALHFHMFQHVVITNIGEYGGTTIQAPFKLPYHRLICHVHGSNQ
                     ISINMADLDLAAFRRNTKTHKEIKSKPAGIKNR"
     gene            complement(11013..12182)
                     /locus_tag="PKZ52_02600"
     CDS             complement(11013..12182)
                     /locus_tag="PKZ52_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNG58686.1"
                     /translation="MERGITGRYQSSIAGGIACQAFIPEPLPPKPPLQLNEKLQSRIN
                     LAMLAMGRLDAISTLLPDAHLFLYSYVRKEAVMSSQIEGTQSSLSDLMLFEMEGSPGV
                     PMDDVQEVSCYVNALNLGVQRTNEGFPISFRLLSELHSALMTSGLGINKSPGEFRTNQ
                     VWIGGHRADEAIFVPTPPQEIANCWADLERFINDLPETTDPLIKAALTHVQFETIHPF
                     MDGNGRLGRMLIPLILVSAKVLKQPLLYLSVFFKKHRQVYYERLQQVRLTGDWENWLL
                     FFVDAVSATATQAVATAQQLYALREQHQQQVMALGRIGPSAQQVLNVLFEKPIANINT
                     LVALSGLTPATVGKVMDKLSQPELALVRECTGQKRNRIYAYSAYIDILNQDLNSL"
     gene            12548..>13064
                     /locus_tag="PKZ52_02605"
     CDS             12548..>13064
                     /locus_tag="PKZ52_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58687.1"
                     /translation="MFKILRLLVLLACAQGAFCAAPSSGDYDGDGAVDVLDVWPTNFA
                     ASVDTDADGAPDFWTSGCDVNCQAQSGLYLDAFPSDRRFAVDENKNGIPDGYREYCDP
                     VCHGPLTAEEITTDVPDADKDGILDNVDTDDNNDGVTDADSDSNGLIDIYTIEQLDAI
                     RYSLRSHSRKID"
BASE COUNT         3163 a         2916 c         3129 g         3856 t
ORIGIN      
        1 atggcgaccc tatgacctta gttgcacgcg gcgccgcttt atacgcagcc accatcggtt
       61 ttaaaaacac gcgacaggca gatgccactg cgcaggcgca cacggccaaa aatgcctgcg
      121 aattatggct gcaatacccc agtgtatgcg ccgatgtaca gccggacatt atggggcgta
      181 ttcttgatgc caaacatttt gcccccgccc gcgtgcaatt aatccgcgac gacggccaat
      241 gggagagcaa ccccattgag ctggatgaaa acggcgtatt ttttatcagt gtgtatttag
      301 tggcggcgaa gaataataaa ttcttaataa aattattgga taaaaaaggc ggcgttatcc
      361 ccgccatgcc agacacaatc gccattgtgc acggtttgac gatttccgat ccacctctat
      421 cgcgctccat tggcgttgct ttagctaatg gccagcgccg cagttttatt gatcgcggca
      481 caccgctgcc cgctaaacgc tcatttttac aatattcagc ggaaacctta gtgcccggca
      541 gcggccagca attaaatatt cccattgtac aaggtgagcg tgcccaggcg cgcttttgcc
      601 gtaaagtggg caacctggtt attcccgcag cgcaattgca gcagcaatta ccggcgggct
      661 cgcccattga aattaccatt gaggtggatc gcggcggtaa tttaaaggcc agcgctttaa
      721 ttgtggatca gaaaaaactc atcaccagcg ttgccgaaat tctaaccccc agcgccgatc
      781 ccaccgcttt gcaggtgggt tttaatcagg tgcaagcgcg cttattgcaa caacagcggg
      841 aggccttcgc ccggcgggat caagccgcaa taagtgtgct ggatcaattg gcgaaaaaat
      901 tacacggcgt gaataaagac ctgttattag ccagcggtga tcaagacgca gccctgcgcg
      961 cccagcgcaa tttgatggag gtggaagcgg aattagaaac ctttgaaagt cgcgatcaat
     1021 tagaagagct gatgcgcgaa tgcgaaattg tttatttaaa tacccgccac caggtagatc
     1081 aatacggcaa cgaggtggaa aagaaaatgc ttgccgactg cgaagtgcgt tttaataaag
     1141 cgattgagct aaaacgcgtg caagaggtgg agcgtttaat tgagcagatg tccaacttgt
     1201 ttaattccgc ttataggcgc aacccggact tttggattga tcaattccac gccgtgtgct
     1261 cacgcttaca cgaggccacc catttggcta aagccaatca gttagcggcg gaggggcgcc
     1321 gttatttggc ggattccaac ctggcggcta ttgaatcctg tgtgcgccaa ttgtggaatt
     1381 taattcctga tgagcgtttg tcgggcgagg aaaatcacca gtctggcatt caataatgtt
     1441 caggtttttt tgcgcctatc aaacaataaa cctgagtttt aagccactat gagcgctgaa
     1501 tttgccagta cacccctatt ttcagcctgg ccattttggg tgctggagct ggacccggcg
     1561 gcggataacc gcagtattga gcgggcattt caaaaattta ccggcagcct gcaattaaat
     1621 attgccaacg cgaaaaatta tatgaccccg ctgggcttgc gcccgcgaga tgaatttctc
     1681 ctgcgggagg ctcgcgccat tctcaccaac ccagaccaac gtgcactggc agaattttgg
     1741 tatatgccag ctaaggtgca ggcggcggcg ccaactcaac aggttgatac caaaccaaag
     1801 ttagattggc gtgaaatttt aaggactcct taatggcagg tggcataggc gtaatcatcg
     1861 caattattat tcgtttcctg cacacctgcg gccaggctca ctatggtact aatccgccta
     1921 caggcttgaa atacttaggc tgtttgatga tgcgtgcgtt attaaggata tttccgtgag
     1981 cgggatcggt cttataatcg cgatattttt aatagtgcga tttttgataa ataaaaacgc
     2041 cgtgaaagac catacacacc cgccaactgc tccgaaagat gtgtgggtct ggctgacgtt
     2101 atttccagta gtcattcttg cttatttatc tgtatttgtg cttgaccaac tctcgattcc
     2161 gagtttaatt gcattagtag tggtgttttc aattgctagc ctaattttgt tcccatcttt
     2221 atttgcacaa gccagtattt atttaggctg ggtaaaagtg agttattggc tggggcgcat
     2281 tgcctataac cgccatcaaa aagacctgtt tgctggtgct ttattttttg gctggcgcgc
     2341 cgcgcaccat gccaaagggg ataaaaaatc ccagggccat gagtggcttg cgcaacgatt
     2401 aaacaattat cgcggcacac ttggttctgg cactatgctg gttcacgcca tgatgaaagc
     2461 agaacccctg gcctataaag acctgcgagc gcaatttatt tggctgcagg gcattaataa
     2521 aaattggctg cccgagaata tcgcccgtgc ggcatttcgg tttattttgg cccgcgcctg
     2581 tagggaagaa gatttccccg aaattcgcgc gcaagcgcgc tattggcagt tggtatgtaa
     2641 aaatcgcttc gctcaatggg ttgatatgta ccattggtcc ctcggggccg atgttattcc
     2701 gcgcatgcta aaacttaaaa taacatggct gtatttttgg gtgggcaata cccaattgcg
     2761 cagcttttta ccgcatctgt ttaaacaaag cgctgcccca accaccagcc aaatgaattt
     2821 agcccagctt aaagaggccg aattaaactt atttcagtcg ggggccaaaa atagccagtg
     2881 gataaacgac gcctggcgca gctatagcgc aggccccatg gccgatgagt ggctgccgcg
     2941 tataaaagcc ctgggcagtt ttaatgaaaa cgaagtgctc tcccgcttgc aacaatccgt
     3001 attggattta attagcctgc gcagtggcga aggggattta gatagcgagg cggccatggc
     3061 ggagcgggat aatggtttta agttgctgca gattaaaatt gccgccatgg ttaaccgctc
     3121 ggagcaaaat aaaatgatga cgggcattca agaatatgag gagtttttag ccctgttgcg
     3181 ggtggccgag cgcttaggca aagacgatca cagccgcgcc caggtgtttt ttcacattcg
     3241 ctcaccggtg tggaattgga tggtggaatt atggaacaac accaaaaacc gccccctggc
     3301 gtttttagcg ggtaattata tggcgccttt agcccgcgag tttggcgata ccgatgccta
     3361 tgaatttttc tccggcatcg ttggccataa atttacctga tggcggcgcc agtttttgca
     3421 tctccccata cttgttattc tctttcccgc gctccgcgtg ggaatgcata tagtaaggcc
     3481 taaaattccc ctcctgtgga ggggtgcccc gcaggggcgg ggtggttcat ggctattttt
     3541 ttgccaacca acatgcagtt aaaaaacgtc cgtgttctat ggctatcaca ggctgttaat
     3601 tcgccttctt aacaaattcc gacttcaacg acatagcccc cacgccatca attttgcaat
     3661 cgatatcgtg atccccatcc acaatgcgaa taccttttac tttagtgccc actttaatca
     3721 ccaaagacga gcctttaact tttaagtctt taataaccgt gacactatcg ccatcgttga
     3781 gcactgtgcc aaaggcatcg cgcacttggc gggtgtcgct ggcctcagca ggggcatcgc
     3841 tccactcgtg ggcacattca gggcagatat acaggccgcc gtcttggtag gtgaattcgg
     3901 acttacaggc ggggcaggga ggtaaactca taaaaaacct tttaaaaaga gagaatgcaa
     3961 tgcgcctgca aagtataagg gttttagcgc cgcacctaaa ccgcaggggc tatttatgtt
     4021 gaaccatatc tgggccggcc tgattctgct ggccctcgca agcgccgccc tgcaagccct
     4081 cacgggggca gacgcgcagg tattagcccg catgactcaa gcccttatcg actgcggcaa
     4141 actcggcttt gagctcgcca tagggcttac cggcatgttg tgtttgtggc tggggttatt
     4201 ggccattgcc gaagccgcag gtttagtggc gctgctctcc aaaggcctgc ggcctttatt
     4261 tgtacattta ttcccgcagg tgccaaaaga ccatccggct atgggcagca tgagtttaaa
     4321 tattgccgcc aatatgttgg gcctggataa cgccgctacc cctttgggcc tgcgcgccat
     4381 ggcgcaatta caaaccctca acccccaccc cgagcaggcc accagcgcac aaaccttatt
     4441 cttaattatt aataccgcat ccgtaaccct gttgccggtc agcgtactca cctaccgcgc
     4501 ccaattaggt gcagctcagc ctgcggatgt atttttacca ctgttattaa ccagcctaag
     4561 cgcaacatta attggagtaa ttgtagcggg cttttgtttg cgccttaacc tgcgccaccc
     4621 tgtggtttta gcctatgggg cagggctgtt tgcgttaatt gccaccgtta ccttaagcct
     4681 taacggcgcc agcagcgagt atatgcagca ggtttcccag tattttagcc aaggcttgtt
     4741 attaattacg gtggcgggtt ttttattggc tggtgtagtt aaaaaagtcc cggtgtacga
     4801 agtatttatc aatggcgcta aagaaggctt tagtaccgcc gtgacactaa tcccttattt
     4861 agtcgccatg tttttagcca tcagcctgtt acgcgcaagc ggcagtttgg atatggcttt
     4921 gcacggcgta aaaacctgtg tgaatttttt cgggttcgat gcccgctggg tagatgccct
     4981 gcccaccatg atattaaaaa gcctctccgg cagcggcgcg cgggccagta tgctggatgt
     5041 tatgcgtgtg cagggggcgg attcgtttgc cgggcggttg gtgagtatta ttcagggcag
     5101 tagtgaaact acgttttatg ttttaactgt gtattttggt tcggtgggga ttcggcgttg
     5161 tggcattgcg ctgggctgtg cgctggtggc ggatgtggcg gcggcggtgt tgggggttat
     5221 ttattgttat gggttttttg ggtgagcaac caagcgaaag ttgcaaatca ataattgttc
     5281 gggagttttg ggtgagtgtc gcgtctttcc tgtcgcgtcc tcggtcaagt tacgcataga
     5341 cgtggtggaa acccagctcg ccttgaaccg tcaaggattc cttgatagtt gcaaccgagg
     5401 tcagcttggc ggttgtcgcc gtaggtaacc attggtttac tgttgatagc tgatattcca
     5461 ttcaacgctg tttcggcact aaggttcgcg cgctgttgcc ctgattctgg ctatcttcgc
     5521 tggtgtagag gataagtagg gtcatacgct gcccttaatt aagcgtttct cacgctcaat
     5581 ctctgcaatt aactcctctt cagttggcaa gatgcgttga tatttagagg caaatagctg
     5641 ttggttttct ttgatcaccg aatatttaac gatggtttca tctttgctat cgcacaggat
     5701 aataccgata gtggggttat catcttcgcc gcgctttaga tcatcaaaca tgcgcacata
     5761 catatccatt tggccgatgt cctgatgagt cagtttgccg gtttttaaat cgatgatcac
     5821 aaaacacttg agtagatagt tataaaacac taagtcgata aaaaaatgac tggtttcggt
     5881 gctgatgcgc atttgcctgg ctacaaacga aaagcccttg cccatttcca gcaggaattt
     5941 ttgcagttca tcaataatgg cttgctctag tttgttttct tttagttgac cagtttcggg
     6001 cagttgcaaa aactccagta cataggggtc tttgataaat tccagatgtg ccgaggttcc
     6061 agaggtggaa gattgcgcgg actgggttga cagcaggcgc tgaaagctct ggctcttaat
     6121 gttgcgctcc agttggcgta ccgtccaatt ttgctctctg gcttctttac aataataatc
     6181 aatggcttgc ggcgactcca cgcgcataat caagcgcagg tggctccagc ttaattctct
     6241 acacactgtg tagagaatgt cttgtttggg gaatgtcagg tagaactggc ggcagttgta
     6301 caaattggcg taggagaagc ctttgccaaa atcgcttgtt aaagcagtag acaggtcttc
     6361 aatcaagcgt ttgccgtatt cggctttaag cttgccctgc tgttcttctt gtacgatgcg
     6421 ctggccaatt agccaatagg cttccaccat ggcagcattt actgccgagc gcgccttgcc
     6481 gcgcgcttgc tcgagtatgg tttttatatc gctgtgaaag gcgcttaagt gactcatatt
     6541 accagcccgt agttatatag gatcggtggc gcaaagacaa aaacggcagt atgtaatcgc
     6601 gataatcgtc agtgttcaca atgatttgcc ctaattcata tggagtgttt ttaatcatgg
     6661 ctatcttccg tataatacaa attggtaatt ttaatgagct ttttaggggg tgtttaaagt
     6721 gaatcatggt tttactggtt gcaaagtttc ctttatttca gacatcgttc tatatgggcg
     6781 caaataaact acggctgctt tgaatcgttt gtcattgtcg cgtaccattt tctagtgttg
     6841 atcctgtggg ttgcttgtca tcattgtttt taattttata actgggttct ctatatctaa
     6901 acgaaggttt tttctggctc ttggctcttg gctcttggga agctggtgtc gttttggtca
     6961 ttattgagtt ttgtgctttt aattgggttg gtgtgcatat tgtttgggtc ggaaaggtga
     7021 ttcggatttc cgattttcca tttgtgctgg ttgatttatc tattttttat acccgctggc
     7081 tttgatttta tttctttgtg tgtttttgtg tttcgtctaa atgctgcaag atctaaatct
     7141 gccatattaa tagatatctg attgctccca tgaacatgac aaatgaggcg atggtaagga
     7201 agtttgaatg gtgcttgaat tgtggttcca ccgtattcgc cgatatttgt aatgactaca
     7261 tgttggaaca tgtggaaatg taacgcggca gccatgctgt caaaagtggc aacgtctgaa
     7321 ttgtgtgcgg ccaccacgaa taaatccgtt ttgtccctta aatctgcagc aagactaata
     7381 tcggttgcgt catagcaaat agcacctgat aatttaaatg gaccttcggg gtgaccgtgt
     7441 acctcaatta aatgctggca aggtcgatag ccttcaactc ctaaaagttt ttcatttggg
     7501 gtcatatatt gtttgccctg atccctgatt atccattggc gagctccttc cttgtaatgc
     7561 ggaataaacc atctggcaat atttactaat tttccattgt ggtctgtgaa aacaagaccg
     7621 gcaaaaatca ttgactgtgt tttgtctgca agacgcttga taatgtcttg atcgtctatg
     7681 tgtatagcca cctcagggaa tacgattaag tcagctgcag gcttactttt gtcagtatct
     7741 gtagcgagct tggcttctaa agttttatat gtgagctggc agattgccat gatatgttcg
     7801 cgatgttttg cgcgagtgat aggattattt agcttaggat ctgcaaggga gaagtcattt
     7861 gtttttggaa gtaattgctg aacggtgacc aatctaaaat tatctttgtc attttttggg
     7921 cgatctatac gggttggtaa taatggtaag tatgaagaac gacaatacat atcatttagt
     7981 ttttttaatc gagatttcag gcacaccaga aaatcttcca aactggcaat ttctcctatg
     8041 tctagattgc tgacatagga tgattgaaat cctggccact gcaagctcac cattaataat
     8101 tcagagaacc agctggtaac tgtggcgaac tcaccaataa ttttttctgg agagtgcatc
     8161 atccccattc ttcgcttgta ccagctggtg cgtagtcctt tataagtgat cgtctttccc
     8221 tttttccatc gatttcccgt gaaatccaca ccgcccaaga ctgatgcgcg aagtatagat
     8281 ccaacccaat atatgattgc tctttctttt tctggcttgc catctaccgt ttctatccag
     8341 ctgggaatgg aaaaacgagg gtcactcaat gatttgccaa ctaaaatgca tttgacttct
     8401 atgtctttat gccaaagttc aatccatgac gtgatttttt ctttgtcgat cattattgaa
     8461 tttggggagg ctgaaattgc ttttggattg ttgcaggcta atttgactag gccaattgct
     8521 aattttagca gtgctgcctc atgttcaaaa ccattttctt cagagcaaat tatatgcgcc
     8581 aatctttgct tggacgcgct gcgttttacg aaacttgaag gtttggccca aagtaattgt
     8641 tcttttacat caagtaattc ttctcgttta tctaaaatct tccagagcga tagccaaaac
     8701 ggcccccctc ttttcgcgaa cggaataatt gcattaattt tatctgttcc ggttttttcg
     8761 atatgctcaa tcaatatcga tgcataacta tctgtattgt cagtgatttg ggatgctact
     8821 tcaaataaag caatggtctc ttgttgaaat cttttaggga ggcttccagc caaaatggca
     8881 tgcaatgttt gttgaatgga atcttcaaat cgttccacta aaatgggttt atttagaaca
     8941 cccaaaagca ttaaagcttg ccgagctaca aatttcttgc tgcttttgct tgaggttgtt
     9001 ttttgagcaa agcgagatat aagttctaga attccagatg gatttgctga attggggtat
     9061 gacactgttt gaaaatagcc atgaaaatca gcagcacagc ggaagagatc tgccaataga
     9121 taatccatca agatacaagt aatcttattt gcgtgttttc cataagtgct gcgggagtaa
     9181 atggcctcga tcacaggctc aaacatatct ggatttggga atatttctat cgctttccgc
     9241 aagaccagtc caagtgaagg gtctttcatc caagctagta tcagtttttt ggcgagcaac
     9301 tcactttcgt tgtcactttt tataaaatag tcagcatctg cattttcatt gctgtgcgcg
     9361 tcaatttttc gtttgcttcg cattatggat tcgagcctgt ttgctgcgaa ccttttcaag
     9421 gtgtctggac gtatgtcatg gtcaaactgg gttagctgaa ctagttttat atctgatttt
     9481 ttattttctt ttaatgcatc aagatcaatt tttgtatcgc tttctatatt caataggcct
     9541 tctaacagcc cagaagagct ttcaagtatg tccctgtcag cagggccgct aagctcgctc
     9601 tgaatcatct tgattcgact ggatatgctc ccagcattat ccagatcaga aagttttgtt
     9661 atttttgttt ttttggggtt tatgcttaaa gctttgtttc cagttttttt aaagattttc
     9721 ccaatccaaa tatttagctc gtcggaaagg gctgcaatat ctgttgattc gctgtgtatc
     9781 actagccgta aatcatctac gtagcggcag tagtcatgaa gagtaattga taaattggat
     9841 gagatgtatc tgttaatgtt ttcgccaatt gccctatcga attcgacgag gtatgcgttc
     9901 gcaaagaaac ctgaggcaac taaaccttga ggtagtccga gctcagtaga aaattctttg
     9961 atttttttgg catattttag agagtcatcg cgccattgcc aattggtaat tcttgcaacg
    10021 gcctgccaga acatatcatc gtagtcgttc ccatattcgc gtgacaatgc ttttaatcgc
    10081 tctatgagaa cggttctgtc aattttgttg tagaattttg aaaggtcaaa actgactacg
    10141 tacacgtttt cttggtcagc acttgattct gatatctgct ttcctattgc tattggtctt
    10201 tctataaaat tttgatagtc ggtaaaaaat tttctatagg tatcactatt tccccagcga
    10261 aaccacgctc tttcgtcctg ccaatcacaa actaggcgat tgccataact ataaacttta
    10321 ttttcttgcg cagatttgaa gctaaaattg gtgttgctgc aatcaccttg agccgtttca
    10381 acagcgtcag ccaagcataa cattactgcg ctagcccaag tttgatctcg gatagttata
    10441 tgtgctaatg gtcgaagagg gggcttcttg tcgtctctgg attttggaac ccaaccttta
    10501 acctcatcaa ctatccaagg ctcgcttttt gcggcgggca ctaactcaat agggtgcagt
    10561 aagcttcctt tttttatctg ctctgcccat ttatgcgcat tctcttcaat ccctagagcc
    10621 gaaatatcca aggctagagt gtcggcatac cagttaaagc ttcgaatata tccgtgggtt
    10681 tttttccatg cttgggtaat tattataggg tcattcaaat aattaatagt tggtttaatg
    10741 gttctatatt tttcggctat caatttcatt ctaatgtgcc cttgctaatg ttttaattga
    10801 taggtaacca tggctcttgg gagatatgtg acaaatttat aagcgcattg tttcttctct
    10861 gcgaagaatg cgcacatcga ccataacatt tgattcctgt atggctgctc gttggtgcag
    10921 accatgcttc cgttgtgaag acacatattt tgaatcgctg taattcattg catatcatct
    10981 cgcaaggcct ctggtgcttt tgcaccctat ctttataggg aattcaaatc ctgattcaaa
    11041 atatctatat aagccgaata agcataaatt cggttacgtt tctgcccggt acattcgcgc
    11101 accagcgcaa gttccggttg gctgagttta tccatcactt tacccactgt ggcgggggtt
    11161 aagccactga gggccacaag cgtgttgatg ttggcgatgg gtttttcaaa tagaacattt
    11221 aacacctgtt gcgccgaggg gcctatacgg cccaatgcca ttacctgttg ttggtgttgc
    11281 tcgcgcaggg cgtagagttg ttgtgcggtg gccacggctt gggtggcagt tgcgctgacg
    11341 gcatccacaa aaaacagcag ccaattttcc caatccccgg ttagccgcac ttgctgtaat
    11401 cgctcgtaat aaacctgccg atgtttttta aaaaacaccg ataaatacag cagcggttgt
    11461 ttcagcactt ttgccgatac cagaattaat ggaattaaca tgcgccccaa gcggccattg
    11521 ccatccataa atgggtggat ggtttcaaat tgcacatggg taagggcggc tttgatcagc
    11581 gggtctgttg tttcgggcag gtcgttaata aaccgttcta ggtctgccca gcagttggct
    11641 atttcttgcg gtggcgtggg gacaaatatc gcctcgtccg cccggtggcc accaatccac
    11701 acttggttgg tgcgaaattc gccggggctt ttattaatgc caaggcctga cgtcattaag
    11761 gcactgtgta attcgctgag caggcgaaag ctgatgggga agccttcatt ggtgcgctgt
    11821 acgccaaggt tgagggcgtt tacgtaacag gacacctcct gcacatcatc catgggtaca
    11881 cctggggagc cttccatttc aaacagcata aggtcgctta aggaggactg ggtgccttct
    11941 atttgcgagg acatcaccgc ttctttgcgc acatagctat ataaaaataa gtgggcgtcg
    12001 ggcagtaggg tgctgatcgc atccaggcgg cccatggcta acatggccaa attgatgcgg
    12061 ctttgcagct tttcattcaa ctgcaaaggg ggcttaggcg gcagtggctc ggggataaat
    12121 gcctggcagg cgatgccgcc tgcaatactg ctctggtagc gaccggtgat accgcgctcc
    12181 atgggggctc caagttaaaa taagaaggtg cgctaattta acttatccgg caagataaaa
    12241 taagggctgc tcgtagttta ggttagccaa tgccaaaagt gtggaattga tagacctaag
    12301 gccgcgcatt gctttggttg aagcacctcg ttcagcagcc tgccaattgc caaccgcctc
    12361 acacatccag cctccttctt cccatcgccg caacttcccg cccttccact tcgtacccct
    12421 gttaaccgtt attacctcca gtagtcactc gcagtaggtg caagttaagg gggtgatttg
    12481 ttgttaccag tacgcctgta ttaaccccat tgatacaacc attggaattt cattatggat
    12541 tacgcttatg tttaaaattc ttcgtttatt ggttttgtta gcctgtgccc aaggcgcttt
    12601 ttgcgctgcg ccaagcagtg gcgattatga cggtgatggt gctgttgatg tgcttgatgt
    12661 gtggcctaca aatttcgccg catcggtgga tacggatgcg gacggcgcgc ctgatttttg
    12721 gactagcggc tgcgatgtaa attgccaggc gcaaagtggt ttatatctgg atgcatttcc
    12781 atcggatcgg cgctttgctg ttgatgaaaa caaaaatggc attccagatg gctaccgaga
    12841 atactgcgac ccggtgtgcc acggtccatt aactgcggaa gaaataacca cggatgtacc
    12901 cgatgcagat aaagatggca ttttggataa tgtggatacg gatgataata acgatggtgt
    12961 aacggatgcc gatagcgatt caaatggatt aattgatatt tacactatcg agcaattgga
    13021 tgccattcgc tacagtctaa ggagccacag ccgtaaaatt gatt
//
LOCUS       DAOQGS010000040        13008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2216_length_13008_cov_4.351810, whole genome shotgun sequence.
ACCESSION   DAOQGS010000040 DAOQGS010000000
VERSION     DAOQGS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_13008_cov_4.351810"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKZ52_02610"
     CDS             complement(<1..660)
                     /locus_tag="PKZ52_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNG58688.1"
                     /translation="MKCVKYGLLILLMQAGSCLAAQQLMPSPAQIEQFKSLSAEEKQA
                     LARSVGIDPASISTNSAQPQIREPEAAPRAAPDKKQAPDAAKAKDAKVKNIGDKDANL
                     KSVESLEDESLNENPVVDVKKPAKDQTVTLKPFGADLFSNGPTSFTPASDIPVPADYV
                     MGPGDNIVIQLYGKENNAYSLTINREGQVQFPEIGPVSLAGLSFSQAQTVLNEVVEKQ
                     MI"
     gene            complement(685..2178)
                     /locus_tag="PKZ52_02615"
     CDS             complement(685..2178)
                     /locus_tag="PKZ52_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNG58689.1"
                     /translation="MRSARRLLFTHAIVACGWFSPLGDLQAAPWAEPNDRALRHAIEV
                     LRDTGLLTGPITAWPIPWQTLAEALDKLPPHELTEQQQQAVSLLNGAVRNADRFKITS
                     QISTATYPRALGGFGDTEREKSAAAISLEAMGEVFAINLSATYSSDASDGDTWRADNS
                     YLSARVGNWGLGIGALERFWGPAWQDSLILSSNARPSPGLFIQRLSPDAFETPWLSWL
                     GPWQLTTFVNQLESARDYSHTKFWGLRITHSPFSFLELGYSRTALFGGTSRPESLSVI
                     TDLLLGRDNRGGDGIADDASNEPGNQLGGFDWRASAHLDNYSGAWYGQVIGEDESGGT
                     PSRNIGTMGLELATATATISHRIWLEGSNTLMRFDRDGMANGTYEHAIYTSGYRYHGR
                     SMGASSDNDSQVYSLGGLHSFEDGDLITWRLGKAYLNRDGSNAPLPGGNPLSERALTL
                     LIADASYQHSLSETSQIIVGGQYLEQPIDARADVLDSNVYITLKSYW"
     gene            2302..2499
                     /locus_tag="PKZ52_02620"
     CDS             2302..2499
                     /locus_tag="PKZ52_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58690.1"
                     /translation="MMPRAAHTNGSAAIAAMSLFHGGMGYEFGMSFGLIHCLVLFVGA
                     ANSGWLAILSKKFPASIFFVD"
     gene            2723..2935
                     /locus_tag="PKZ52_02625"
     CDS             2723..2935
                     /locus_tag="PKZ52_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNG58691.1"
                     /translation="MATRELGTVKWFNNARGYGFITRGESTDDIFVHYRNIRGEGYRS
                     LAEGQKVEFEVHEGEKGLQADDVACL"
     gene            3188..6652
                     /gene="mfd"
                     /locus_tag="PKZ52_02630"
     CDS             3188..6652
                     /gene="mfd"
                     /locus_tag="PKZ52_02630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNG58692.1"
                     /translation="MSTASLVVAPSLPKKIGDKLTWGNAQGSGRAWAIAEAACAQASS
                     GLVICTNMADAYRLELELSLFLKDRPNAPKLSHFADWETLAYDNFSPHQDIISTRMRS
                     LYELAKGERVLVVASVSTLLQRLSPKNYILGNSLVLKVGEKLPLARLREQMVQAGYNH
                     VDTVYQHGEFAIRGGLLDIFPMGASIAYRIELFDDEVETLRPFDPETQRTTGTIECVE
                     LLPAKECPLDRASVTRFKNRWMELFNSDDPRRCPIYQDVASGLSPAGVEYYLPLFFDE
                     TASLFDYLPSDTCTYVLSDLDLSLEQFWVDATGRYESRKGDRLRPLVPPRQLFLSPEE
                     FYAGLKPLSRINLLAAPAAERAGHANLPLQSLTNLAIDARAIEPLGALELFLAESNQR
                     ILFCAETSGRREAVLDQLAKLNFKPVAVDSIFAFSQGNSQYAITLADLSSGINLPHIR
                     VIAEAELFGLRIQQKRRRSKAQDDHDSIIKNLTELKMDAPVVHIDHGVGRYRGLQSIE
                     VDGQTAEFLVLEYAEQAKLYVPVANLHVISRYSGSDDDHAPLHRLGGELWAKAKRKAA
                     EQIRDVAAELLEIYAKREARKGFAFEDPEEAYQQFAASFPFEETPDQEAAINAVRQDL
                     ISAKPMDRLVCGDVGFGKTEVAMRAAFIAAQNSKQVLVLVPTTLLAQQHYENFKDRFA
                     DWPVNVDVISRFRSGKETEEVRERLASGKVDILIGTHKLLSKEVKAPNLGLLIIDEEH
                     RFGVRQKEALKALRAEVDILTMTATPIPRTLNMAMNGIRDLSIIATPPAKRLSIKTFV
                     RESDEQTIKEAVLREIMRGGQVYFLHNEVKTIEKTAADLAVLIPEARIQIGHGQMRER
                     ELEQVMSDFYHKRFNVLLCTTIIETGIDVPNANTIIIDRADKFGLAQLHQLRGRVGRS
                     HHQAYAYLLTPNAKNMTEDAHKRLTAIMEAQDLGAGFTLASHDLEIRGAGELLGEDQS
                     GQIQTIGFSLYMDMLERAVKALREGKEINLDEPPQSPVDINLRIPALLPDDYLPDVHT
                     RLILYKRMAGLKTPRDIDDMQIEMIDRFGLLPEPAKNLLRVAALRIKCEQLGIVKIEA
                     NRSFGRIEFGPKTQIDPLLLVKLVQSQPQHYKFDGATHLRFNYDMETSQERLITTENT
                     LKALGARD"
     gene            6673..7344
                     /locus_tag="PKZ52_02635"
     CDS             6673..7344
                     /locus_tag="PKZ52_02635"
                     /inference="COORDINATES: protein motif:HMM:NF022425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HNG58693.1"
                     /translation="MHRNFSRVTLLILGISLSALASAQSLYQVEVIIFERKGLVRTLG
                     ASQTTDGRNLAYLENAQVLKTQEPSDAQTSPAFTPLADSELTLKPQATSLERSKQMRV
                     LLHQAWLQPIGRQPIGVILAGGAPFGPWHELAGTLQLSLDKTLQLNSALWYAQFMPDN
                     GHPITNPITIPLPPEQGALSDAKTEGFFATLAVPFSFKQNIKPGVTTYVDHPIFGLII
                     KVQPL"
     gene            complement(7891..8583)
                     /locus_tag="PKZ52_02640"
     CDS             complement(7891..8583)
                     /locus_tag="PKZ52_02640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG58694.1"
                     /translation="MTHLKHKWALITGSSRGIGQQIALGLAAQGCNIILHGRDSASVQ
                     KTRALLAGFEIEIAEACGPLDSPESVAAIIAAVNAGPGRVDILYNNAAIMTPGRTMWD
                     IPHSDWLQTFQVNLYAVVQLCNAFAPAMAARGFGRIINITSGIKDQPDLTVYSVSKGA
                     IDKYTQDLAAQMRGTNVLVSHVDPGWLRTDLGGPNAEHAVETVLPGALMPALLPDNGP
                     NGQFFAAQDYRA"
     gene            9273..10574
                     /locus_tag="PKZ52_02645"
     CDS             9273..10574
                     /locus_tag="PKZ52_02645"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008889 - glycerophosphodiester
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNG58695.1"
                     /translation="MPRTLMNTAAMLLLTLALPMVAQADRNADENRFDDERNLDHAWS
                     REGGYPSLSVQLGPRPFYLVEDMDASPLKRKLQACALGKAIYKPTQFSIGHRGAALQF
                     PEHTQQSYEAAAKMGAGIIECDVTFTKDKQLVCRHSQCDLHTTTNILAVPELAAKCRQ
                     PFVPADELTGKPASAECCTTDITLAEFKSLSGKMDGFNPKAKTPAEYLQGTPAWRTDL
                     YASQGTLMTHAESIQLIKKLGAKFTPELKSASVAMPYEGTYSQAMYAQQMIDEYKAAG
                     INPRDVFAQSFDGADIRFWIKNEPKFGRQAVYLDDANTPAELPSAADLKALKAEGVKI
                     VAPPLWALVKLDNKNKIVPSDYALNAKAAGLEIITWSFERAGPLKNGGGWYYQTVNSA
                     LNNDGDMMEVLDVIARQVGVIGVFSDWPASVSFYANCMGLE"
     gene            complement(10882..12078)
                     /gene="rluF"
                     /locus_tag="PKZ52_02650"
     CDS             complement(10882..12078)
                     /gene="rluF"
                     /locus_tag="PKZ52_02650"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF007784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HNG58696.1"
                     /translation="MPSQSSTRLNKFISDSGICSRREADRLIEQGKVYINGKKAKIGD
                     VVYPGDRVNANGQLLEEKSAEDFIFIAFNKPVGVTSTTERSDKTNIIDYINHSQRIFP
                     IGRLDKDSQGLIFLTNNGDIVNKILRAGNRHEKEYLVTVNKPITTQFIDGMGNGVPIL
                     GEMTKKCQVSQETPYIFRIILIQGLNRQIRRMCEYFGYEVTKLERVRIMNIRLKGLPL
                     GDWRDLDEKEIASVFKMLEHSSSEETGKKTTFKPKIDRPTKNTANTATKAPATQKQTA
                     AQKSAPGYKPSESKIPKPSGKALPFHEHAASKKNARNKNTSNPFSQSGTTAEKAVLGN
                     KATRRSADWKKLANSQQKPTGGKPTNIKGPKSAPAKHSSAQGAGKNKASTKPYKSSGR
                     PGSSKK"
     gene            complement(12163..>13008)
                     /locus_tag="PKZ52_02655"
     CDS             complement(12163..>13008)
                     /locus_tag="PKZ52_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNG58697.1"
                     /translation="NSKGKILMPNPFYQIYEGAALLAGCQPVFLNCTQESNFLPDFDA
                     VSSDTWRDCQVLFICTPGNPTGKIIPLATLQKLIALADEYNFIIASDECYSELYYGDK
                     PPGLLEACASLGRTDYRRCVVFHSLSKRSNLPGLRSGFIAGDAHILQQYLLYRTYQGC
                     ALPVPTQLASQLAWQDEVHVEANRAAYAEKFATAQNILSPHIELTEPEGAFYYWLKTP
                     IKGEDFARALFSAQNVTVLPGAYLSRVCQGINPGEDYVRMALVAEINECREACERLRD
                     FLHAG"
BASE COUNT         3025 a         3140 c         3447 g         3396 t
ORIGIN      
        1 gatcatttgt ttttctacca cctcatttaa tacggtttgc gcctggctaa aactcaaacc
       61 ggcgaggcta acggggccta tctcaggaaa ttgcacctgg ccttcgcggt tgatggtaag
      121 ggagtaggcg ttgttttctt tgccatacag ttgaatcact atgttatcgc cagggcccat
      181 aacatagtcc gcaggcacag gaatatcgga ggcgggggta aagctggtag ggccattgct
      241 gaacagatcg gcaccgaagg gttttagggt gacggtttga tctttggcgg gttttttcac
      301 atcaacgact gggttttcat tgaggctttc atcctccaaa gactcaacac tttttaggtt
      361 ggcgtctttg tcgcctatgt tcttaacctt ggcgtccttg gctttggcgg cgtctggagc
      421 ttgttttttg tcgggagctg cgcgcggggc cgcttcgggt tcccgaattt gcggctgcgc
      481 actgttagtg ctaatgctgg ctgggtcgat acccaccgag cgggccaggg cctgtttttc
      541 ctccgccgag agggatttaa attgttcgat ttgggctggc gatggcatca gttgctgggc
      601 ggcaaggcag ctgcccgcct gcatcagcag gatcaaaagg ccgtatttaa cacacttcat
      661 agagggtccg ttaggtttgg tcatttacca gtagcttttt aaggttatgt aaacattgga
      721 gtcgagcaca tccgcgcgcg cgtcgatggg ctgctcaagg tattggccgc ccacaatgat
      781 ttggctggtc tcggataaag aatgttggta actagcatct gctattaaca gcgttaatgc
      841 ccgctcgctt aaggggttgc cgccgggtaa gggagcattg ctgccatccc ggttgagata
      901 agccttgccc aaacgccagg tgatgaggtc gccgtcttca aaactgtgta agccacctag
      961 gctatacact tgggagtcat tgtcgctgct ggcacccatg ctgcggccgt ggtagcggta
     1021 gccgctggtg taaatggcgt gttcgtaggt gccgttggcc ataccatcgc ggtcaaagcg
     1081 catcagggtg ttggatccct ccagccagat acgatggctg atggtagcgg tagcggttgc
     1141 caactcaagc cccatggtgc cgatattgcg ggagggggtg ccgccggact cgtcctcgcc
     1201 gatgacctgt ccgtaccagg cgccgctgta attatccaag tgagcgctcg cccgccaatc
     1261 gaagccgccc agttgattgc ccggttcgtt ggaggcgtca tcggcaatgc catcaccgcc
     1321 gcggttatcc cggcccagca gtaagtcggt aatgacactt aaggactcag gccgggaggt
     1381 gccaccgaac agtgcagttc ggctataacc aagctctaaa aaactgaacg ggctgtgggt
     1441 aatgcgcagc ccccagaatt tggtatggct gtaatcccgc gccgattcca gttggttgac
     1501 gaaggtagtc aattgccaag ggcccagcca gctgagccag ggggtttcaa atgcgtccgg
     1561 cgagaggcgc tggataaaca ggccggggga ggggcgggca ttgcttgaga gaatcaagct
     1621 gtcctgccac gccggccccc aaaagcgctc aagcgcgcct atgcccaggc cccagttgcc
     1681 cacccgggcg cttaaatagc tattgtctgc acgccaggtg tcgccatcgc tggcgtctga
     1741 gctgtaggtt gcggagaggt taatggcaaa tacttccccc atggcctcaa gggatatggc
     1801 ggcggcggat ttttcccgtt ccgtatcacc aaagccaccc aaagcgcggg ggtaggttgc
     1861 agtggatatt tggctggtga ttttaaaccg gtcggcattg cgcaccgcac cgttgaggag
     1921 ggagaccgcc tgttgctgtt gctcagtgag ttcgtggggc ggcaacttat cgagcgcttc
     1981 tgcgagtgtt tgccagggta tgggccaggc tgtgatcggc ccggtgagca ggccagtgtc
     2041 gcgcagcact tcaatggcat ggcgcagcgc ccggtcattg ggttccgccc agggggccgc
     2101 ctgtagatcg cctagggggc tgaaccagcc gcaggcaaca atggcgtgtg tgaagagcaa
     2161 tcgtctggct gagcgcatgg gcgtggctgc aaaaaagggc gcatcttaga ggcttttggg
     2221 gccgagggga atgtttgcgt gttagatggc ggcaaactga gcccgcctcg ggggtagggc
     2281 aagtgggtaa taggcgcgcg cgtgatgccg agagcggcgc acacaaacgg cagcgccgcc
     2341 attgcggcta tgtcgttgtt ccacgggggc atggggtatg agtttggcat gagttttggc
     2401 ctgattcatt gccttgtatt gttcgttggg gcagcaaata gtggctggct ggcaattttg
     2461 tcgaaaaagt ttccagcttc gatatttttt gttgattagt atcactttta aaatgtgatt
     2521 catatcttat tttgtggcgc tgataaacaa attggcgcgt caattaacct gtttcccagt
     2581 cattctgtag acaagtgtaa aaaattatcc tatttttcca ctgctttagt agggaagggg
     2641 tgcggctaaa caggcttgtc gccttcattc cttttgatga aactaggttt taattttaat
     2701 aataaagatg aggtacggaa gtatggcgac tcgtgaatta ggtacggtca aatggtttaa
     2761 caacgcgcgc gggtacggat ttattacgcg tggagaatct acggacgata tctttgttca
     2821 ctaccgcaat atccgcggcg aggggtaccg atccctagcc gaagggcaga aagtagagtt
     2881 cgaagtgcac gaaggggaga aaggtttgca agcggatgat gtagcctgcc tttaatttag
     2941 ggcgacaggc ataattgtaa aaacccaggt tacctgggtt ttttttcgcc ggtcatttaa
     3001 ccacctccta atactcgaag gcggcctacg tggtttttta agcgtatcaa ttaattagat
     3061 tccatattcc aacccggcaa gtgtttgatt tgatgctcgc cacccagggg ctgcccctag
     3121 tgccaaaata ggccggagct tcctgctaga atgccgccct cgttttcccg gccctaagac
     3181 cctcgccatg agtacagctt ctcttgttgt tgccccctcc ttgcctaaaa aaatcggcga
     3241 taagcttacc tggggcaatg cccagggcag cgggcgggcc tgggccatag ctgaggccgc
     3301 ctgtgcacaa gcctccagcg gcctggtgat ctgtaccaat atggccgatg cctatcggct
     3361 tgagctggaa ttgtctttat tcctgaagga tcgccctaat gcccccaaac tcagtcattt
     3421 cgccgactgg gaaaccctcg cttacgataa tttttccccc caccaagaca tcatctccac
     3481 ccgtatgcgt agcctgtatg aactggccaa gggcgagcgg gtattggtgg tggccagtgt
     3541 tagcaccttg ctgcagcggc tgagccctaa aaattacatt ctgggcaaca gcctggtact
     3601 taaagttggg gaaaaattgc ccctggcacg cctgcgggag cagatggtgc aggcggggta
     3661 caaccatgtg gacacggtgt accaacacgg cgagttcgcc attcgcggcg gcctattgga
     3721 tatctttccc atgggtgcca gcatcgccta ccgcattgaa ttatttgatg acgaagtgga
     3781 aaccctgcgc cccttcgacc cggaaaccca gcgcaccact ggcaccatcg aatgcgttga
     3841 actcctgccc gccaaagagt gcccccttga tcgcgcttcg gtaacccgtt ttaaaaaccg
     3901 ctggatggaa ttgttcaaca gcgacgaccc gcgccgctgc cccatatacc aggatgtggc
     3961 ctcaggcttg tctccagccg gtgtggaata ttatctgccg ctgttttttg acgagacggc
     4021 aagcctattt gactacctgc cctcggacac ctgcacctat gtgttaagtg acttggattt
     4081 aagcctggag cagttttggg tcgatgccac cggccgctat gaaagccgca agggggatcg
     4141 cctgcggccc ttggtgccgc cccggcaatt atttttaagc ccggaagaat tctacgcagg
     4201 ccttaaaccc ttaagccgta ttaacctgct agccgccccc gccgctgagc gggccggcca
     4261 cgccaatttg cccttgcaga gcctaaccaa tctggccatt gacgcccgcg ccattgagcc
     4321 tttgggggcg ctggagttat ttttagccga aagcaatcag cgcattttat tctgtgcaga
     4381 aacatcgggc cgccgggaag cggtgttgga tcagctggca aaattaaact ttaaaccggt
     4441 tgcagtggat tccatttttg cttttagcca aggcaatagc caatacgcca ttaccctcgc
     4501 cgatttaagc agcggtatta atctgccgca catccgcgtg attgccgagg cggaattatt
     4561 tggcctgcgc attcagcaaa aacgccgccg cagtaaggcc caggatgacc acgacagcat
     4621 cattaaaaac ctcaccgagc taaaaatgga tgcgccggtg gtgcatattg accacggcgt
     4681 gggccgctac agaggtttac aaagtattga agtggacggc caaacagcgg aatttttagt
     4741 gctggaatac gccgaacagg ccaagttata tgtgccggtg gccaatttac atgtaattag
     4801 ccgctattcc ggctcagatg acgatcacgc gcctctgcac cgtttaggtg gtgagctttg
     4861 ggccaaagcc aagcgcaaag ccgccgagca aattcgcgat gtggcggctg aattgttaga
     4921 aatttacgcc aagcgcgaag cgcgcaaagg ttttgcattt gaagatccag aagaggccta
     4981 tcaacaattt gctgccagtt tcccgttcga agaaactccc gatcaagaag cggcaattaa
     5041 tgcggtgcgg caggatttaa tcagcgcaaa acccatggat cggctagtgt gcggcgatgt
     5101 ggggtttggc aaaactgaag ttgccatgcg ggcagccttt atcgccgcgc aaaattccaa
     5161 acaggtttta gtcttggtgc ccaccaccct gttggcacaa caacactatg aaaattttaa
     5221 agaccgcttt gccgattggc cggttaatgt ggatgtgata tcccgttttc gcagcggcaa
     5281 agaaaccgaa gaagtgcgcg agcggctcgc cagtggcaaa gtggatatat taattggcac
     5341 ccataaatta ctcagcaaag aagttaaagc gccgaattta gggctgttaa ttatcgacga
     5401 agaacaccgt tttggcgtgc ggcaaaaaga agctttaaaa gcattacggg ccgaggttga
     5461 tattctcacc atgacggcca cacccatacc gcgcaccctt aatatggcca tgaacggtat
     5521 tcgcgattta tcgattatcg ccacgccacc ggctaagcgg ttatccatta aaacatttgt
     5581 gcgcgaaagc gatgagcaaa ccattaaaga ggcggtgctg cgggaaatta tgcgcggcgg
     5641 ccaggtgtat tttttacata atgaagttaa aaccattgaa aaaaccgctg cggatttagc
     5701 ggtgttaatt cctgaggcgc gcattcaaat cggccacggc caaatgcgcg agcgcgagct
     5761 tgagcaggtg atgtcggatt tttaccacaa gcgttttaat gtgttgctct gtaccaccat
     5821 tatagaaacc ggtattgatg tacccaatgc caataccatt attatcgacc gcgcggataa
     5881 gtttggcctt gcccagttac accaattgcg cggccgggta gggcgctctc accaccaggc
     5941 ctatgcgtat ctgctcacgc cgaacgctaa aaacatgacc gaagacgccc ataaacgcct
     6001 aaccgccatt atggaggcgc aggatttagg cgccggtttt acccttgcct cccacgattt
     6061 ggaaattcgc ggcgccgggg agttgttggg ggaagatcaa agtgggcaaa ttcaaaccat
     6121 tggtttttcc ctttatatgg atatgctgga gcgagcggta aaagctctac gcgaaggcaa
     6181 agaaataaat ttagatgaac cgccccagtc cccagtggat attaatttgc gtatcccagc
     6241 tttattgccg gatgattatt tgccggatgt gcacacgcgg ttaattcttt ataaacgtat
     6301 ggcaggttta aaaaccccgc gggacataga cgatatgcaa attgaaatga ttgaccgctt
     6361 cggccttcta cctgagcctg ctaaaaacct attgcgggtg gcggccttaa gaattaagtg
     6421 cgagcagtta ggtattgtaa aaattgaagc caatcgctca tttggccgta ttgaatttgg
     6481 ccctaaaact cagattgacc ctttattatt agtaaaatta gtgcaatccc aaccccagca
     6541 ttataaattt gatggcgcca cgcatttgcg ttttaactat gatatggaaa ccagccaaga
     6601 gcgcttaatc accaccgaaa acacccttaa agccctcggc gcccgcgatt agtgcccgac
     6661 agaggaattc ctatgcaccg caatttttcc cgtgtcacgc tgttgatatt aggtataagc
     6721 ctgagcgcct tagcgagcgc gcaaagcctt tatcaagtgg aggttattat ttttgagcgc
     6781 aagggtttag tgcgcactct aggggcgagc caaaccaccg atgggcgtaa cttggcctat
     6841 ttagaaaatg cccaagtact aaaaactcag gagcccagcg atgcacaaac ctcgcctgcg
     6901 tttacccctc ttgccgacag cgaattaacc cttaagccac aggccacaag ccttgagcgc
     6961 tccaaacaaa tgcgtgtgtt actgcatcag gcctggctgc aaccgattgg ccgccaaccc
     7021 ataggtgtga tcctcgccgg cggcgcgccc tttggaccct ggcatgaatt agcgggcacc
     7081 ttgcaattaa gcctggataa aaccctgcaa ttaaacagcg ccctgtggta cgcccaattt
     7141 atgcccgata acggccaccc gattaccaac cccattacca ttcctttgcc gcccgagcag
     7201 ggcgctctta gcgatgctaa aacagaaggt ttttttgcca ccttggcggt gccgtttagt
     7261 tttaaacaaa acattaaacc tggtgtaacc acttatgtgg accatccgat ttttggctta
     7321 ataattaagg tgcagccgct gtgatgagcg gttgtatttg gcatgggagt tttggtgggc
     7381 gctaagctgc gattcgcagc taaacgagtt tgcacaaagg tgtggagtag gcacacagga
     7441 tatgaccgtt gattgctatt ttttgtaccg ccagaattta gaaagtcatt ttgcagaagt
     7501 tccttgttgt cattaagtga ttgtatggaa gaagaatgaa taatcggcgg gcggctagcg
     7561 tccttgtgca gggtataacc attctggcgg caattgctct tttggtttgg ttcttggggt
     7621 cttgcctgtt gagaattgtc ggtatggcaa tgtgtataat cggtttgttt cagattagat
     7681 attgcaatca gtgggacgat attttggaga ggtatctgcc ttggaatcat ctcaggcatc
     7741 atgcgtttgt ttatttgggc tccggcatta ttcttgtggt ggtaggtgtt cacttaataa
     7801 tttattgcca cttcttaagc ggcgacgctc agcctaaatt tgccttgcca gaccgtcgcc
     7861 gcctatgtgt ttcaacaagc tgctaagcaa ttatgctcgg taatcctgcg ccgcaaaaaa
     7921 ctgcccattg gggccattat ccggcaataa agcaggcatc agtgcaccgg gtaatacagt
     7981 ttccaccgcg tgctccgcat taggcccgcc taaatccgtg cgcagccaac ctgggtctac
     8041 atggctgact aacacgttgg tgccgcgcat ttgggcggca aggtcttggg tgtatttgtc
     8101 tatggcgcct tttgacacac tataaacagt taaatccggc tgatctttaa tacccgaagt
     8161 aatattaata atgcgcccaa agccgcgcgc tgccatggcc ggtgcgaacg cattacacaa
     8221 ttgcaccacc gcatataaat tgacttgaaa ggtttgcagc cagtcgctgt ggggtatatc
     8281 ccacatggtg cggcccgggg tcatgatggc ggcattgtta tagagaatat ccactcggcc
     8341 ggggcctgca ttcacagctg caataatagc ggctacgctc tcggggctgt ctaatgggcc
     8401 gcaggcttcg gctatttcaa tttcaaaccc ggctagcagc gcccgtgttt tttgaacgct
     8461 ggctgagtcc ctgccatgca aaataatatt gcagccttgg gcggcgagac ctaaagcgat
     8521 ttgttggcct atgccgcggc tggagccggt aataagtgcc catttatgtt ttaagtgtgt
     8581 catagatatt ctcgtataat acgattagtg tgtttctggc tttgggagta atttatttaa
     8641 tcgtcaattg ctaataatta tcagtgcttt cattgttgtg tgagatcaca caaactcata
     8701 gcgaatttca caagaaggta tttctgcttt tccattgtat cgcaactctt gtgactgtcc
     8761 tgttcgcgat gtctcaagct gactggtttg cgaaatggcg tgaattttct agctgaagtt
     8821 atatgctggt gaatacctta agcctatttt atgtgtattg gaataacatt gggcgaagtt
     8881 tttcgggatt tatttgtggg ataaatccta tttcaattta tacctgaata gaggcatgct
     8941 gattaatttg agttgcgcta ttaaaaggtc tgcgtatgta gaatcgattt tcatggagct
     9001 tcaatcgtgt atgcataagc aaataaaatc atatggtttt attgaaaata agatgcggct
     9061 cgaattaatc ccaatgccat ttaattttag gctaaggtct gttgagtatg gataatttat
     9121 ccatttgtgt ttgccggaga gcgcttatgg tcgagtgttt gaggtgcaca taattaactt
     9181 gtcataaaac tggcaagtct tcgtcacaat cctggggtag ggttctgggg ccttaaggcg
     9241 tcgctattca tccaccactc gaggattaac tcatgcccag aacccttatg aacacagctg
     9301 ctatgctgct gctcactctg gccctaccca tggttgcaca ggccgaccgc aacgccgatg
     9361 aaaatcgttt tgacgatgag cgaaatcttg atcacgcctg gagccgcgaa ggcggttacc
     9421 caagcctgtc cgtgcaattg gggccgcgcc cgttttattt ggtggaggat atggatgcga
     9481 gccctttaaa acgcaaatta caggcctgtg cgctgggtaa agccatctat aaacccaccc
     9541 agttttccat cggccatcgc ggtgcggcct tgcaatttcc tgagcatacc cagcaatcct
     9601 atgaggctgc cgccaaaatg ggcgccggta tcattgagtg cgatgtgacg tttaccaagg
     9661 ataaacaatt ggtttgccgt cactcccaat gcgatttaca caccaccacg aatattttgg
     9721 cggtgccgga attggccgct aaatgccgtc agccttttgt gcctgccgat gagctaaccg
     9781 gcaaacctgc atctgccgaa tgttgtacca ccgatattac tctggcggag tttaaaagcc
     9841 tgagcggcaa aatggatgga tttaacccca aggccaaaac accggctgaa tatctgcagg
     9901 gcacaccggc ctggcgcacc gatttatacg ccagccaagg cacgcttatg actcatgcgg
     9961 aaagtatcca gctgataaaa aaactcggtg ctaaattcac cccagaatta aaatcggcca
    10021 gtgtcgccat gccctatgaa ggaacctaca gccaagctat gtatgcccag caaatgattg
    10081 atgaatacaa ggcggcgggc attaatccgc gggatgtttt tgcgcaatcg tttgatgggg
    10141 cagatattcg tttttggatt aaaaacgagc ctaaatttgg ccgccaagcg gtgtatttag
    10201 atgatgccaa tacaccagca gaattgccca gcgcagctga tttaaaagca ttaaaggcag
    10261 aaggggtaaa aattgtggcg ccacctctat gggcccttgt taagctggat aataaaaata
    10321 aaattgtgcc ctctgattat gcgctgaacg ctaaggcagc aggtttggag attattacct
    10381 ggtcatttga gcgcgcaggc ccgcttaaaa atggcggcgg ctggtattat caaaccgtaa
    10441 actcggcatt aaataatgac ggtgacatga tggaagtatt ggatgtcata gcgcggcaag
    10501 tgggggtgat cggcgtgttt tctgactggc cagccagcgt aagtttttac gccaattgca
    10561 tggggttgga atagcgcccc aggtgtagat tatgtgtaac tattcagtac ttatttattg
    10621 taactggaca tgattctcgc accgactgga atccagcaga cgaatgcttt aatgcgcaag
    10681 ccccgcagtg atgaatgcag gtcatttcag acgcgccgtg aatacatccg tgtaggctcg
    10741 tcaaaaaggt ccctcttttt gacggtctgc aataacctgc cttcagccct gctgcaccgc
    10801 gaatcgattt tgccgcttgg ctaatttccc ctcaatttgc cgtccgcgtg gcgagggtga
    10861 caaggtgtta aatagttacc attatttttt agaagatcca ggccgccctg agcttttgta
    10921 aggtttggtc gaggctttgt tttttccagc accctgggca ctgctgtgtt tagcgggggc
    10981 agacttaggc cctttgatat tggttggttt tccgccagtg ggtttttgct ggctgttagc
    11041 cagttttttc cagtcggcac ttctgcgggt ggctttgttg cctaatacgg ctttttcagc
    11101 cgttgtccca ctctggctaa agggattgct tgtattttta ttacgggcat tttttttact
    11161 cgcggcgtgt tcgtggaatg gtagcgcttt gccgctgggt ttgggtattt tgctttccga
    11221 cggtttatat ccgggcgcag atttttgtgc agctgtttgt ttttgtgtcg ctggcgcttt
    11281 ggttgctgtg ttggcggtgt tttttgttgg tcgatctatt ttgggtttaa atgtcgtttt
    11341 ttttcctgtc tcctcggatg aagagtgctc taacatttta aatacagagg cgatctcctt
    11401 ttcatccaga tcgcgccaat cccccagagg caaacccttt aggcgaatat tcataatgcg
    11461 tacccgttcc aatttggtga cttcatagcc aaaatattcg cacatcctgc ggatctgcct
    11521 gtttaacccc tgaattaaaa taatgcgaaa aatataaggt gtctcctggc tgacctggca
    11581 ttttttggtc atctcgccaa ggatcggcac gccattgccc atgccgtcaa taaactgtgt
    11641 ggtgatgggt ttattgactg tgaccaggta ttctttttcg tggcggttgc ctgcgcgcaa
    11701 tattttattg actatatcgc cgttgttggt gagaaaaata agcccctgcg agtctttatc
    11761 cagccgcccg atggggaaga tgcgctggct gtggttgata taatcaatga tattggtttt
    11821 atcgctgcgc tcggtggtgc tggttacgcc caccggttta ttaaaagcaa taaaaataaa
    11881 atcttctgcg gatttttcct ccagcaattg gccgttggcg ttgaccctgt cacctgggta
    11941 taccacatcg ccaattttgg cttttttacc attgatatac accttgccct gttcaattaa
    12001 gcggtccgcc tcccggcggg agcaaatgcc tgaatcgctg ataaatttat ttaggcgtgt
    12061 agatgattga cttggcatag gcgtttatta aggtgcaaat gaaaaaggtg aagaagtctt
    12121 tacaggagta tgcaaggccc gctgaaagcc aggtaggcag ttttatcccg cgtgcagaaa
    12181 atcccgcaag cgctcacagg cctcacggca ttcgtttatt tccgccacca gcgccatgcg
    12241 tacatagtct tcgcccggat taatgccttg gcagacgcgc gataaataag cccctggcaa
    12301 taccgttaca ttttgcgcgc taaaaagggc gcgggcaaag tcttcgcctt taatcggcgt
    12361 ttttaaccaa taataaaaag cgccttccgg ctcggttagt tctatgtgcg ggcttaaaat
    12421 attctgcgcg gtggcgaatt tttccgcgta ggcggcgcgg ttggcttcta cgtggacttc
    12481 atcttgccag gctaattggc tggctagctg tgtgggcacc ggcagggcgc agccttggta
    12541 ggtgcggtag agtaaatatt gctgcagtat gtgcgcatcg cccgcaataa accccgagcg
    12601 taggcccggt aaattggaac gtttggatag actgtgaaac accacgcagc ggcggtaatc
    12661 cgtgcgccca aggcttgcgc aggcttctaa tagtcctggt ggtttatcac cgtaatacag
    12721 ctcggaataa cactcatcgg aggcgataat aaaattatat tcatccgcca gcgcaattaa
    12781 cttttgcagt gtggccaggg gaataatttt accggtggga ttgccggggg tgcagataaa
    12841 taacacctgg cagtcccgcc aggtatcact gctgacggcg tcaaaatccg gtaaaaaatt
    12901 gctttcttga gtgcaattta aaaataccgg ttggcagccc gccaagaggg ccgcgccttc
    12961 atagatttga taaaaagggt tgggcattaa aattttgccc ttagagtt
//
LOCUS       DAOQGS010000041        12729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2291_length_12729_cov_5.529825, whole genome shotgun sequence.
ACCESSION   DAOQGS010000041 DAOQGS010000000
VERSION     DAOQGS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_12729_cov_5.529825"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /gene="gspJ"
                     /locus_tag="PKZ52_02660"
     CDS             complement(<1..372)
                     /gene="gspJ"
                     /locus_tag="PKZ52_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HNG58698.1"
                     /translation="MPPNTPRGFTLIEVLIAIVIMAIIATLTGQALHTATTSSDATND
                     AVKRLNSVDRTWTLLEADLRNTLPRAIVPSMGEPIPPLIANRSSDYWLRVFRGGVVNP
                     LHAPRSELVRIGYRLTDNVLWR"
     gene            complement(381..779)
                     /gene="gspI"
                     /locus_tag="PKZ52_02665"
     CDS             complement(381..779)
                     /gene="gspI"
                     /locus_tag="PKZ52_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNG58699.1"
                     /translation="MNGVKNKHTSRQASLGFTLVEVMVALIIVAMAVPALLMRMQSMA
                     NSSIHNRNVTTAHWVAENRLVEVMLTRDMERREPRGREAGDMMMSGNKWDWNVETVDT
                     KNSEFPGFEMTVRAAPQGQETMADLVVYLF"
     gene            complement(760..1359)
                     /gene="gspH"
                     /locus_tag="PKZ52_02670"
     CDS             complement(760..1359)
                     /gene="gspH"
                     /locus_tag="PKZ52_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HNG58700.1"
                     /translation="MGSVAMPKPQRGFSLMELMAVLLVVGLALGIVTVSVGHGGPEKQ
                     LYDKLDQFLIQSDFAVEHAVVSGEALGILLEPPDWQVAANPELDRDEIGWKYRWQILA
                     EKGWQDVPNMPAVSLPPDIEIDVTINKKRWDWHAKTTDKKSPVISISPSGEITPFVLE
                     IKHALVRDFVQHIELSEEGTIVWREAAEDEKKRDERRKK"
     gene            complement(1365..1811)
                     /gene="gspG"
                     /locus_tag="PKZ52_02675"
     CDS             complement(1365..1811)
                     /gene="gspG"
                     /locus_tag="PKZ52_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNG58701.1"
                     /translation="MTIKREGGFSLIEIMVVIVIMGLLLSVVGPAVFQNISQAKTETA
                     KAHMASMKTALQSYRLDNYTYPTTEQGLQALVTKPTTEPVPRKWRSEGYMEKLPLDPW
                     DRPYVYTMPSESHPFDIVTLGADGQPGGEGEDADIGYWDEPGEKKE"
     gene            complement(1874..3106)
                     /gene="gspF"
                     /locus_tag="PKZ52_02680"
     CDS             complement(1874..3106)
                     /gene="gspF"
                     /locus_tag="PKZ52_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNG58702.1"
                     /translation="MGAFNYQALDANGKTVKGVIEGDSDKQVRALLRQRQLRPLEVQA
                     AQQRVPIGTNAKPKRFGFSSAPKLGYKDIALVTRQLASLIQSGLPLDEVLQATATQSK
                     KPAVKSLMLQVRARVMEGLSLAQAMSEAPKAFNNLYRAMVRAGESAGFLGPVLDQLAD
                     YTERSQEARQKIQMAMIYPIVLMVVCCGVVTGLMAFVVPKLIGLFKNSKKDLPFITDI
                     LIASSNFIVNYGVFLLIGIIGLVVLFKHLLKEPSRELRWHKFLLKWPLIGELIRTSEA
                     ARYSSTLGLLVKSGVPLLEGLRIASQVLGNRHIQKASLAVAVSVQEGMSFNKALDQCG
                     EFPPLLVQMVASGEANGQLAEQLLHVAANQERELSFTLGTLMGLMEPAMILIMAGIVG
                     FVVMAIMLPIFQLNQMVH"
     gene            complement(3109..4587)
                     /gene="gspE"
                     /locus_tag="PKZ52_02685"
     CDS             complement(3109..4587)
                     /gene="gspE"
                     /locus_tag="PKZ52_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNG58703.1"
                     /translation="MENESALAQPVAFTKLPFAYASANQVLVEENRILHTEATSHRVL
                     IELRRHLGRSFTAEELDLPAFKARLTQSYQSGDGEALQAVEDMGAEMDLSALAEDIED
                     GELLSGEGDAPVIRLINAILSQAVTEKASDIHIEPYEDRVSIRFRVDGVLKEVLSPKP
                     QLGPVLVSRLKVMARLDIAEKRVPQDGRITVKLAGHGVDLRVSTMPSAHGERVVLRIL
                     DQAAGAISLDKLNMPTRVLEDFKTVLAKPHGIILVTGPTGSGKTTTLYSGLSHLNTRA
                     RNILTVEDPIEYVLPGIGQTAVNTKVDMTFARGLRAILRQDPDVVMIGEIRDRETAAI
                     AVQASLTGHLLLSTLHTNTAIGAVMRLQDLGIEPFLLSSSLEALMAQRLVRKLCNECK
                     QSHVATDAECERINIPKGSVIYSPVGCEKCDYRGYKGRTGIYELIPVDDVLRQLIHEG
                     VGEQQMLAHARKFSRPIDADGRDKVLAGITSIEELLRVTVTS"
     gene            complement(4598..6622)
                     /gene="gspD"
                     /locus_tag="PKZ52_02690"
     CDS             complement(4598..6622)
                     /gene="gspD"
                     /locus_tag="PKZ52_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNG58704.1"
                     /translation="MKNIIKMSAACVLSVAVSINTAWAEQTWTVNFKDSDIQEVVKFV
                     ADVTGRTMVVDQRVRGRVKVISAKPLNEAELLQLFRTVLEMNDFTMIDVEGVSRIIPL
                     KDARSSPIPVLNQGASDKGYITKVIQLKNIAAAKVLPVIRPLIPQHAHLAAYDPSNSI
                     VIADEAENIDRIIDLIERIDKSAMPVTDIVELRYANATDMVQTLTNLDKVDSQNLPQN
                     ALRITADKRNNAVLVTGEDVQRQRVKALIAKLDKPKAQAGNVRVVYLNYAKAKSVAET
                     LTKVVQNMSKLAPPGAPEGGGQPQAPISATVEADEATNALLITASGDALVALLGVIER
                     LDIRRAQVLIEAIIVSMDDGKARELGVQWMARNKDGMFGGSGLDDKRGAAAATAAGAA
                     LKAGENPNAEQLAGLAGSVLSFPGQILGVLGTNQSDDFAVLLNALEKDTKNNILSKPS
                     ILTTDNTEAKFSVGKKVPFKTGSYSGTGSNTGGGSNSFSSPFTTIQREDVGITLKVKP
                     HVNEGNKILLDIAQEISDVYADPAASDLVTNQKKLETQVLAANGETIVMGGMIEDKNT
                     RTEQRVPVLGRIPVVGRLFRSNSKDNSKNNLMIFLRATVIRDDEALRGATAEKYDYIR
                     QQQLGQRKIGAFRGEDKTLSVLPELVQPELQKEPVIVTPGAAKADAPSEQ"
     gene            complement(6633..7583)
                     /gene="gspC"
                     /locus_tag="PKZ52_02695"
     CDS             complement(6633..7583)
                     /gene="gspC"
                     /locus_tag="PKZ52_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HNG58705.1"
                     /translation="MVQLSQFADTPLVARLSKESHKVVSFLSKVPTERWRAIVLALAL
                     IWSVQSLAKLFWLVMPQPKSGPEQLVPTNVNAGVQAKAPLNIDGLTSAHLFGDYDAEK
                     MRAAQADQQAGENVNQITQLDLKLQGVIMASDPEKSWAIIGEENSQQLFKIGDSIPPA
                     NGVTLEKVEDLRVILNNNGKREALWLYGEDGMNVASSRQAAAAPPPPPPPANVQQATV
                     TQEQLQSATSIGDVVRFMVATEGGKMIGYKVRPGRKRELFDQVGLKNDDIVISVNGIE
                     VNEPQKVREVYQALKTASEANLQVLRDGGTQSIQIRMSSG"
     gene            7983..9575
                     /gene="gshA"
                     /locus_tag="PKZ52_02700"
     CDS             7983..9575
                     /gene="gshA"
                     /locus_tag="PKZ52_02700"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNG58706.1"
                     /translation="MPAKHFIAQLAEPANRSLLSGILRGIEKEGLRVDATGHLSHTAH
                     PQGLGSALTHPYITTDYSESLLELITPPCHRLDTLLKWLNDLHRETYQQLGAEYIWSN
                     SMPCILPQEHAIPIAQYGSSNRGTMKSIYRLGLGHRYGRTMQTVAGIHYNFSLPEAFW
                     ALLQQAEEDHGLLEDFISRRYFDLIRNFRRHYWLLVYLFGATPAVDASFLAGREHQLE
                     HLGTDTFFLPFATSLRMGDLGYQSRAQESLHVCYNNSRSYVQSLVTAINTPYPDYEKK
                     GVIGTDGRYQQLNTGLLQIENEFYSAIRPKRTAKSGETALSALCRRGVEYIEVRCIDI
                     DPFSASGVNAEQLRFIDAFLLHCLLSPSAATSPQETREILHNQQQVVSKGRAPGLNLT
                     INGQQTTLTEAANQLLDSIKPWAELLDMSGDGSLVSASWQRQKDKLLDPSTTPSAQAL
                     AAIKSTGSFKQWALSCAKANQQAFTKDASAHHDSPYFAALAENSLRQQEQEETADAAV
                     PFTDYLAKYYAQYHACCRGTIE"
     gene            complement(9798..10241)
                     /locus_tag="PKZ52_02705"
     CDS             complement(9798..10241)
                     /locus_tag="PKZ52_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNG58707.1"
                     /translation="MIGIFCCKPVQKKLAVLLLCAATVAVPAFANSGGGAAEGAAGAV
                     YVTFKPPLVTNYGGAGRIKYVKADMSIRVENAAVADSVQHNMPLIRNNLLNIMAKQTD
                     ESLGGQPGKEAMRQEALKEIQAILKQEANLEGVLDLYFNSIVVQK"
     gene            10341..10835
                     /locus_tag="PKZ52_02710"
     CDS             10341..10835
                     /locus_tag="PKZ52_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNG58708.1"
                     /translation="MPLPSIRYTFLIIALGTLGLMLTGYYMQFVMKLIPCPLCITQRF
                     FIIAVGITALAAFFHQPQKLGRRLYASLGAILAIIGGGFSSRQVWLQSLPPDQVPACG
                     PDLSYLIENFPLKEAFMVLLRGDGNCAEVTWTFLGVSIAGWTLVAFSGLMLINLWQWV
                     RAER"
     gene            11172..11630
                     /gene="rsd"
                     /locus_tag="PKZ52_02715"
     CDS             11172..11630
                     /gene="rsd"
                     /locus_tag="PKZ52_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HNG58709.1"
                     /translation="MLENCKTARERWGGVSVIIDRWLQERQDLLVLFCALSETSSTET
                     QANRLPQFFQLLVDYVSAGHFEVYEQLVQEGREFDDQEGLKESESLFDKIQETTDALL
                     DLNDKYQETDDLDALKEDLSKLGEYLESRFELEDRIIEVLHVSHKDRVIG"
     gene            complement(11810..12643)
                     /locus_tag="PKZ52_02720"
     CDS             complement(11810..12643)
                     /locus_tag="PKZ52_02720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNG58710.1"
                     /translation="MNTPFLRKSCLLSVAMASAVFLAGCNKDQDKAAAKPEAAPAQVA
                     LNTMEEKVSYIVGFRMASQAKASGFTLDKKTLSQAITDAQEGKDAQIPQEEQQKIMTE
                     FQQQQETKHQEEMKKVGEENLKKGQDFIAENGKKEGVKTTASGLQYKVIKASEQKDAK
                     SPKPEDTVKVHYHGKLIDGTVFDSSVDRGEPVTFPVNGVIPGWVEGLQLMKVGDKFEL
                     AIPANLAYGAGGTGPIPPNSALVFEVELLEINPKQEEAPANPHGKAAESAKPAEAKSA
                     K"
BASE COUNT         3015 a         3584 c         3078 g         3052 t
ORIGIN      
        1 ccgccacaac acattgtctg tgaggcgata gccgatgcgc accagctcac tgcgcggcgc
       61 gtgcaggggg ttaaccacac cgccgcgaaa cacccgcagc cagtagtcgc tggaacggtt
      121 ggcaatcaaa gggggaatag gctcgcccat ggagggcaca atggcgcgcg gcagggtatt
      181 gcgaaggtcg gcttccaaga gggtccaggt gcggtctacg gaatttaacc gcttcaccgc
      241 atcgttggtt gcgtcgctac tggtggtggc ggtgtgcaat gcctggccgg tcaaagtggc
      301 aataatcgcc ataatcacga tggcgattaa tacttcgatc agcgtaaacc cacggggggt
      361 gttgggcggc atgtatttca tcagaacaga taaaccacca aatcagccat ggtttcctgc
      421 ccctggggag ccgcccgcac ggtcatttca aaaccgggaa attcagagtt tttggtatcc
      481 accgtttcaa cattccagtc ccacttattg ccagacatca tcatatcgcc agcctcccgg
      541 ccgcgcggct cgcgccgctc catatcgcga gtgagcatga cctccactaa acgattttca
      601 gccacccaat gggccgtagt gacgttgcga ttgtggatgg aggagttggc catggattgc
      661 atgcgcatca agagggcggg caccgccatg gcgacgataa tgagcgcaac catcacctcg
      721 accagcgtga agccgaggct agcctggcgg gaggtgtgct tattttttac gccgttcatc
      781 acgctttttc tcatcttccg ccgcctcgcg ccaaacgatt gtcccctctt cagacagctc
      841 aatatgctgc acgaaatcac gcaccaacgc gtgtttgatt tccaaaacaa agggcgtaat
      901 ttcgccactg ggcgatatag aaatcaccgg ggatttttta tccgtggttt tagcgtgcca
      961 gtcccagcgt tttttattga tggtaacgtc gatttcaata tccggcggca gggaaacagc
     1021 cggcatattc ggcacatcct gccagccttt ttccgccaga atttgccagc gatatttcca
     1081 gccgatttca tcgcgatcca gttcagggtt ggcagctacc tgccaatccg gcggctccag
     1141 caaaatcccc agggcctcgc cggacaccac cgcgtgctca acagcaaaat cgctttgaat
     1201 taaaaattga tccaatttgt cgtaaagctg tttttccggc cctccgtggc ccaccgatac
     1261 ggtcactatc cccaatgcca ggccgactac taataacacg gccatcaact ccattaagga
     1321 gaagccccgc tggggcttgg gcattgcgac tgaccccatg ggttttattc ctttttctct
     1381 ccgggttcat cccagtaacc tatgtcagca tcttcgcctt caccaccggg ctggccgtcg
     1441 gcgcccaggg tcactatgtc aaaggggtgg ctttcgctcg gcatggtgta cacatagggt
     1501 ctatcccagg ggtctagcgg gagtttttcc atgtagcctt ctgagcgcca tttacgcggc
     1561 acaggctcag tggtgggttt ggttaccagc gcctgcaggc cctgctcggt ggtgggatag
     1621 gtgtaattgt ccaagcgata agattgcagc gccgttttca tggaggccat gtgggccttg
     1681 gcggtttcgg tttttgcctg cgagatattc tggaacacag caggccccac cacactcagc
     1741 aacaggccca taattacgat cactaccatg atctcgatca ggctgaatcc gccttcacgt
     1801 ttaatagtca tatttccccc taaaggaatg ttcgaattga aaagaagagt ggtattccta
     1861 aaccttagga tgcctagtgc accatttggt ttaattggaa aataggcaac atgatagcca
     1921 tcacaacaaa gccaacgata cccgccatga tgagaatcat cgccggttcc atcaggccca
     1981 tcaaggttcc taaggtaaaa gacagttcgc gctcttggtt ggccgcaaca tgcagcagct
     2041 gttcagcgag ctgaccattg gcctcaccgc tcgcaaccat ttgcactaac agaggtggaa
     2101 actcgccgca ctggtccagg gctttattaa aactcatacc ctcttgaaca gacacagcca
     2161 cagcaaggct ggctttttga atatgccgat tgcccaaaac ctggctggca atccgcaagc
     2221 cttctaacaa tggcacgccg cttttcacca gcagccctag ggtgctggaa taacgcgccg
     2281 cctccgaggt gcgaataagc tcaccgatca gaggccattt caacaaaaac ttatgccagc
     2341 gcaactcgcg gctgggctct ttgagcaaat gtttaaacag tacaaccagg ccgataatac
     2401 cgatcaacaa aaacacaccg taattaacaa tgaaattact gctagcgatc agaatatcgg
     2461 taataaaagg caggtctttt ttactgtttt taaacagccc tatcagcttc ggcaccacaa
     2521 acgccatcaa gccggtgacc acaccacaac acaccaccat caaaacaatt gggtaaatca
     2581 tcgccatttg gattttctgg cgggcctctt ggctgcgctc ggtgtaatcg gccaattgat
     2641 ccaacaccgg gcctaaaaag cccgccgact cccccgcgcg aaccatggcg cgatagagat
     2701 tattaaacgc cttgggcgct tcagacatgg cctgagccag cgaaagcccc tccatcacgc
     2761 gagcgcgcac ctgcagcata aggcttttaa cagcgggctt tttgctttgc gtggccgtcg
     2821 cctgcagcac ttcatccaaa ggcaggcccg attgaattaa cgaggcgagc tgccgcgtca
     2881 ccagggcaat atccttgtaa cccaatttgg gcgcgctgga gaagccaaag cgcttaggtt
     2941 tggcattggt gcctatgggc acccgctgct gggccgcctg tacctccagc gggcgcaatt
     3001 gccgctggcg caacagggcg cgcacctgtt tatccgaatc cccttcaatg acacccttga
     3061 cggttttgcc attggcatcc agcgcttgat agttaaatgc acccatggct aggaggtcac
     3121 cgtcacacgc aataattctt caatgcttgt gatcccagcc agcaccttat ccctgccgtc
     3181 ggcatcgatg ggccgagaga atttgcgcgc atgggccagc atctgctgct cgcccacccc
     3241 ttcgtgaatc agctggcgca agacatcatc aactgggatt aactcgtaaa taccggtacg
     3301 gcctttataa ccgcgataat cacacttttc gcagcccaca gggctgtaaa tcacactgcc
     3361 tttgggaata ttaatgcgct cgcattccgc atcggtggcc acatgggact gcttgcattc
     3421 gttacacagc ttgcgcacca gccgctgcgc cattaacgcc tcaaggctcg acgacaatag
     3481 gaacggctca atacccaggt cctgcaaacg catgacagca cctatggccg tgttggtgtg
     3541 cagtgtggat aacaataagt ggcctgttaa actcgcttgc acagcgatgg ccgccgtttc
     3601 ccggtcgcgg atctcaccga tcattaccac atccgggtct tgccgcaaaa tcgcccgcag
     3661 tccgcgcgca aaggtcatat ccactttggt attcaccgct gtttggccga tccccggcaa
     3721 cacgtattca atggggtctt ccacggtgag aatattgcgc gcccgagtat ttagatggct
     3781 taaacctgag taaagggtag tggttttacc cgaaccggtt gggccggtta cgaggataat
     3841 gccgtgaggc ttagccaaca cggttttaaa atcttccaac acccgggtcg gcatgttgag
     3901 tttatccaat gagatagcgc ctgccgcctg gtcaagaata cgcaatacca cccgctcgcc
     3961 gtgagctgag ggcatagtcg atacccgcag gtccacgcca tggcccgcaa gcttcacagt
     4021 gatacggccg tcctggggta cccgtttttc agcgatgtcc aagcgcgcca taacttttaa
     4081 acgcgacacc aacaccggcc ccaactgggg tttgggggaa agcacttctt ttaacacgcc
     4141 atccacacga aagcggatgg aaacacgatc ctcgtagggc tcaatgtgaa tatccgaggc
     4201 tttttccgtt acagcctgag acaaaatagc gttgattaaa cggatgaccg gcgcatcccc
     4261 ctcgccactt aataactcgc cgtcttcaat gtcctcagcc agagcagata gatccatctc
     4321 cgcgcccata tcctccacag cctgcaaggc ttcgccgtca ccgctttggt aggactgggt
     4381 gaggcgcgct ttgaaggccg gcaaatccaa ctcctccgcg gtaaaactgc gccctaagtg
     4441 gcggcgcaat tcgattagca ccctatggct cgtggcttcg gtatgcaaaa tgcggttttc
     4501 ctccaccaaa acctggttag cggaggcata ggcgaagggg agcttggtaa aagctaccgg
     4561 ctgcgctagt gcagactcat tttccataga acccccgtta ctgctcgctc ggagcgtcag
     4621 ctttggcggc accgggggta acaatcaccg gctctttttg aagctcaggc tggactaatt
     4681 ccggcaatac cgaaagcgtt ttatcctcac ctctgaaggc cccaatcttg cgctgaccca
     4741 attgctgctg gcgtatgtag tcgtactttt cagcggtggc gccgcgaagg gcttcatcat
     4801 cacgaatcac ggtagcccgc aggaaaatca tcaaattatt tttactattg tctttcgagt
     4861 tgcttctaaa cagtcgcccc acaaccggaa tgcgacctag cacgggaacc ctctgctcgg
     4921 tgcgagtatt tttatcctcg atcataccgc ccataacgat ggtctcaccg tttgcggcca
     4981 gcacctgagt ttcaagtttc ttctggttcg tcactaaatc tgacgcagca ggatcggcat
     5041 agacatcaga tatttcctga gcgatgtcca aaagtatttt gttaccttca ttaacgtgcg
     5101 gttttacctt caacgtaata cctacatctt cacgctgaat cgtcgtgaac gggcttgaga
     5161 agctattaga gccgccaccg gtattgcttc ccgtacctga atagctgcct gttttgaagg
     5221 gaaccttttt accgacagaa aacttagctt ctgtgttatc agtagtgagg atggaaggtt
     5281 tggaaagtat attgttctta gtatcctttt ctaaggcgtt caacagcaca gcgaagtcgt
     5341 cactctgatt agtacctagc acccctagta tctgtccagg aaatgacagc acgcttcccg
     5401 ccaagcccgc aagctgctcc gcgttcggat tctcccctgc cttcaacgca gctcccgcgg
     5461 cggtagccgc cgcagcgcca cgcttatcat ctaagccgga accgccgaac atgccatcct
     5521 tattcctcgc catccactgg acccctaact cacgcgcctt gccatcgtcc atgctgacaa
     5581 taatcgcctc tatcaaaacc tgcgccctgc gaatatccaa gcgttcaata actcccaaca
     5641 aagcaactaa tgcatcgccg gaagcggtaa ttaacaacgc attagtggcc tcatccgctt
     5701 ccaccgtggc agaaataggc gcctgtggct gaccgccacc ttcgggcgca ccggggggcg
     5761 ctaacttgct catgttctgc acaacttttg tcagcgtctc ggcaaccgac ttagccttgg
     5821 cataatttaa ataaaccaca cgcacattgc ccgcctgagc tttgggctta tctaacttgg
     5881 caatcaacgc tttaacgcgt tggcgctgaa catcttcacc ggttaccagt accgcgttat
     5941 ttcgtttgtc agcagtgatg cgcagggcgt tctgcggtaa attttgtgaa tcgactttat
     6001 ccaggttggt gagggtttgc accatatcgg tggcattggc atagcgcagt tcaactatgt
     6061 cggtaaccgg catggcggat ttatcgatgc gttcaatcaa atcaatgatg cggtcaatgt
     6121 tttccgcttc gtcggcaatc acaatggagt tagacggatc gtaggcggcc aaatgggcat
     6181 gctgtggaat tagcgggcgg attaccggga gaaccttagc ggccgcgata ttcttcaact
     6241 ggatcacttt cgtgatatag cctttgtcgc tagcgccctg gttcagcacg ggaatgggcg
     6301 acgagcgcgc gtcttttaag ggaatgatgc gagagacgcc ttccacatca atcatggtga
     6361 aatcgttcat ttccagaaca gtgcgaaaca actgcagcaa ttcagcctca tttaaaggct
     6421 ttgccgaaat tacctttact cgaccgcgaa cgcgctgatc caccaccata gtgcgaccgg
     6481 ttacgtccgc tacaaacttg acgacctctt ggatatcgga gtctttgaaa ttaacggtcc
     6541 aggtctgctc agcccaggcc gtgttaattg aaaccgcgac gctcaataca cacgcggcac
     6601 tcatttttat tatgtttttc aaaatgaact ccctaaccag aactcatacg gatttgaatg
     6661 gattgggtgc cgccatcacg cagcacctgt aaattggcct cactggcagt tttcaacgcc
     6721 tgatagactt cccgcacctt ctgcggttcg tttacctcga taccattgac cgagataaca
     6781 atatcgtcgt ttttcaggcc cacttgatcg aacaattcgc gcttgcgccc tgggcgaacc
     6841 ttatagccga tcattttgcc gccctcggtc gccaccataa agcgcacaac atcaccgata
     6901 gaggtggcgc tttgcagctg ttcctgtgtg accgtagcct gttgcacatt ggccggtggg
     6961 ggtggcggtg ggggcgccgc tgccgcttgg cgagaagagg ccacgttcat accgtcttca
     7021 ccgtacaacc acagtgcttc gcgcttgccg ttgttattaa gaattacccg taaatcctct
     7081 accttttcga gggttacacc attggcaggc ggaatgctgt cgccaatttt aaatagctgc
     7141 tgagagtttt cttcgccaat gatggcccag gatttttccg ggtcactggc cataatgaca
     7201 ccctgcagtt tcaaatcgag ctgggtgatt tgattaacat tttcaccggc ttgctgatcc
     7261 gcttgggcgg cgcgcatttt ttcagcgtca tagtcgccaa acagatgggc actggtgaga
     7321 ccgtcgatat tcaagggggc tttggcttgt acgccagcgt ttacgttcgt aggcaccagc
     7381 tgctctgggc cacttttggg ctgcggcatc accaaccaaa ataacttggc taagctctgc
     7441 accgaccaga tcaacgcgag tgccagcact atggctcgcc aacgctcggt cggaaccttg
     7501 ctcaggaaag acacaacctt gtgggactct ttagagagcc tggccaccaa aggcgtatcc
     7561 gcgaattggg ataattgcac catttttctc cgtcaggggg ttagtaaggg ccgctgtcga
     7621 tgccatttaa gccgagtaac aggccgggcg ccggatgtag cctaacacgg cacatgacac
     7681 caacacatcg tacgcctact aaaatggttg ttattagtac gcctaaatat gtcccatccg
     7741 cgcgcttact gtaaacactt aaaaacaaat atagccttta aattccacaa tttgggctat
     7801 accgctcatc ctgtgattgc tgttttacag cattcttatg agccgtcatg aggcctttac
     7861 ctgggacgcg cagcctaggc ttaagtttag cccttcgcag caatcccgct aaataaatcc
     7921 tccccccttg taaagggcgg tacttaaccc aattttcggc cgatcacatt tgaggtcatc
     7981 ccatgcccgc caaacacttt attgcccagc tggctgaacc tgccaaccgc tcgttgctca
     8041 gcggcatact gcggggtatt gaaaaagaag gcctgcgggt ggatgcaacc ggccatctat
     8101 cccacactgc acatccgcag ggcctgggtt ctgctcttac acacccctac atcaccaccg
     8161 attacagcga atccctgctg gagctcatca ccccgccctg ccaccgctta gacaccctgc
     8221 tgaaatggct gaatgattta caccgcgaga cctatcagca gctgggcgcg gagtacattt
     8281 ggagcaattc gatgccctgc atcttgcccc aggagcatgc cattcccatc gctcagtacg
     8341 gctcgtccaa tcgcggtacc atgaaaagca tttaccgcct cgggctcggc caccgctatg
     8401 gccgcaccat gcaaaccgtt gccgggattc actacaattt ttcgctgccg gaggcatttt
     8461 gggcactgtt acaacaggca gaagaagatc acggcctgct ggaggatttt atctctcggc
     8521 gctacttcga tttgattcgc aatttccgcc gccactattg gcttttggtc tacttatttg
     8581 gcgccacgcc cgccgtggat gcgagcttct tggcaggccg agagcaccaa ctggagcacc
     8641 taggtaccga tacgtttttt ttgccctttg ccacctcgct cagaatgggg gacttaggct
     8701 accaaagccg cgcccaggag tccctgcatg tgtgttacaa caacagccgc tcctatgtgc
     8761 aatcgcttgt taccgccatt aataccccct atccggacta cgagaaaaaa ggggtgatag
     8821 gtacggacgg gcgctatcaa caactcaata ccggcctgtt gcaaattgaa aatgagtttt
     8881 acagcgccat tcgccccaag cgtacagcca agagcggtga aacagccttg agcgccctgt
     8941 gcaggcgcgg cgtggaatac attgaagtgc gctgcatcga catagatccg ttctccgcca
     9001 gcggtgtaaa tgctgagcaa ctgcgtttta tcgacgcgtt tttgctgcat tgtctgctct
     9061 cgcccagcgc ggcaacctcc ccgcaggaaa cacgcgaaat actccacaac cagcaacagg
     9121 tcgtaagtaa agggcgcgca cccggcctca acctcaccat caacggccag cagacaaccc
     9181 ttacagaggc cgccaatcaa ctactggaca gtattaaacc ctgggcggaa ctcctagata
     9241 tgagcggcga tggcagcctg gttagcgcca gctggcagcg acaaaaagac aaactgctcg
     9301 atccttctac aacaccatct gcccaagcgc tggcagctat caaatcaaca ggcagcttca
     9361 agcagtgggc gcttagctgc gcaaaagcca atcaacaggc ctttacaaag gatgcatccg
     9421 cccaccacga cagcccttat tttgcagcgc ttgcagaaaa ttctctgcgg cagcaagagc
     9481 aggaagaaac agcggacgca gctgtgccct ttacagacta tttggccaag tattacgcgc
     9541 aataccacgc ctgctgcaga ggcactattg agtagaggaa acttcggtta attaatgttt
     9601 tgaagccttc gcaggcagga agctcacagg gagttacaga ggttgaacta gtgcgttttc
     9661 acaatcactg ttgctcaaag gctcctgaag aatttaataa caacgggcca atacatggcg
     9721 ttaacagctc cgtccgcaac ttttttaaca cccatttaga gcggcctaac cgcgcgcgaa
     9781 agtgaaacgc accgccgtta tttctgcacc actatgctgt tgaaatagag atccaaaaca
     9841 ccttcaagat tggcttcctg cttgaggatg gcttgaatct ccttcagcgc ctcctggcgc
     9901 atggcttctt tacccggctg gccaccgagg ctctcatcgg tttgtttcgc cataatattg
     9961 agcagattgt tgcgaatcag cggcatgtta tgctgcacgg aatcagcaac agccgcattt
    10021 tccacccgaa tcgacatatc ggctttgacg tatttaatgc gccccgcgcc accataattg
    10081 gtcaccagcg gcggcttaaa ggttacataa acggcgccag cggccccctc agccgcaccg
    10141 ccaccgctat tggcaaaggc cggcaccgcg accgtggcgg cgcacagcag taacaccgct
    10201 aatttttttt gcaccggctt gcaacaaaat atgccaatca ccgcctaatc cccctaaaat
    10261 tgcgctccct taaatactag accagttctg cccaacccat cagcgcgaat taatccacca
    10321 ccagagcgga aatacacacc atgcccctac cttcaattag gtacacattt ttaatcatcg
    10381 cattaggtac cctcggcttg atgttaactg gctactacat gcagttcgtc atgaaattaa
    10441 ttccctgccc gctgtgcatt acccagcgct tttttattat cgccgtgggc atcaccgcct
    10501 tagccgcttt ttttcaccaa ccgcaaaaac tcggtcggcg cctatatgca agcctaggcg
    10561 ccatcttagc gattattggt ggcgggtttt ccagccgcca agtctggctg cagagcttac
    10621 cccccgatca agtgcccgcc tgcgggcctg atttaagtta tttaatcgag aatttcccac
    10681 ttaaagaggc ctttatggtt ttgctgcgcg gcgatggtaa ttgcgccgag gtcacctgga
    10741 catttctcgg cgtgagcatt gcggggtgga cactcgtagc gtttagcggg ctcatgctga
    10801 ttaacctctg gcagtgggtt cgcgctgagc gctgatgccc aaccctggga tgaataaggc
    10861 cattaaatac acgcaacagt taataaattt cattccgcgc aagaatcttt gcgctaagcc
    10921 ttatgcggtt gtatcgctcg ccagctgcaa tcagcgcgag cattacttcc gcggcttctt
    10981 ttttaaatct caatagagcc tgattggatt caactgctaa actctaaact caggccttgc
    11041 gataggcgct tgcgatagcc acgaagtctt ggataggcac tggggccttg cgatagccac
    11101 taaagtcccc taatgtggaa gcccaagtgt tccactccat ttaaccccgt cactgttgag
    11161 gattctcctc catgctagaa aattgcaaaa ccgcccgcga gcgctggggt ggtgtaagcg
    11221 tcatcatcga tcgctggcta caagagcggc aagatttatt ggttctcttt tgtgccctga
    11281 gcgagaccag ctcaactgaa actcaagcca accgactgcc gcagtttttt cagcttttag
    11341 tggattatgt ttcagccggg cacttcgaag tatatgagca actagtacaa gaaggccgcg
    11401 agttcgatga tcaagagggc cttaaggaga gcgaaagcct gttcgataaa attcaagaaa
    11461 ctacggacgc cctgctggat ctaaacgaca aataccaaga aaccgacgat ttggatgcgc
    11521 ttaaagagga tctatctaaa ctcggtgaat accttgaatc gcgctttgaa cttgaagatc
    11581 gaattatcga agttctgcat gtgtcccata aagaccgagt aataggctaa taacacagct
    11641 cattagtatt gataaaggcc gctgatagcg gcctttttta tgcgtgcgca taaatatgta
    11701 aatcaagcgg gcgattgatc accgcaaagc gccccaaggc gagcaatgat gaatataccg
    11761 cacacaaaaa agcccgccaa aaggcgggct tttttgtgca atccgctgct tacttagcgg
    11821 atttggcttc agcaggttta gctgactcag ccgccttgcc gtgtggatta gcaggagctt
    11881 cttcttgctt aggattaatt tccaacaact caacttcaaa aactaaagcg gagttgggtg
    11941 ggattgggcc agtgccgcca gcgccgtaag cgagattggc agggatggcc agttcaaact
    12001 tgtcgcccac tttcatcaat tgcaaacctt caacccagcc aggaattaca ccgttgacag
    12061 ggaaagtaac aggttcaccg cggtcgacgg agctgtcaaa cacagtgccg tcgattagct
    12121 tgccgtgata atgaacctta acggtatctt caggcttagg agatttggcg tctttttgct
    12181 cgctggcctt gatgactttg tactgcaaac cgctagcagt agttttcaca ccttctttct
    12241 taccgttctc agcgatgaaa tcctggcctt ttttcaggtt ttcttcaccg acttttttca
    12301 tttcttcttg atgcttagtt tcttgttgtt gctgaaactc agtcataatt ttttgctgct
    12361 cttcctgagg gatttgagcg tctttacctt cttgcgcatc agtaatcgct tggctcaagg
    12421 tctttttatc cagagtaaag ccgctagctt tggcttgaga cgccatgcga aagccaacga
    12481 tgtaggaaac tttctcttcc atggtgttaa gagcaacttg agcgggcgcc gcttcgggtt
    12541 tggcagcagc cttgtcctgg tctttgttac aaccggccag aaaaacagcg ctcgccatgg
    12601 caacgctcaa caaacaggac ttgcgcaaga acggagtatt cataaattta aattcccaat
    12661 gaaatgaatt aaagagcttg atttcgaatc tgccaggacc cacacggcac caggcggcat
    12721 gttgaaggg
//
LOCUS       DAOQGS010000042        12450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2361_length_12450_cov_4.439371, whole genome shotgun sequence.
ACCESSION   DAOQGS010000042 DAOQGS010000000
VERSION     DAOQGS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_12450_cov_4.439371"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKZ52_02725"
     CDS             <1..609
                     /locus_tag="PKZ52_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG58711.1"
                     /translation="AETLPAGIEFEWTELTYQEVLAGNTAIYILPLCLLLVFLVLAAQ
                     YESLALPLVVVSIVPLSILSALFGVWVSGGDNNIFTQVSLFVLAGLASKNAILIGEFA
                     RELEIRGMATYQAAITASRMRLRPILMTSFAFIMGVVPMVFSTGAGAEMRSVMGIAVF
                     AGMLGVTFFGLFFTPIFYVLARKLEGKKAAVPAPLINSEYSL"
     gene            606..2093
                     /locus_tag="PKZ52_02730"
     CDS             606..2093
                     /locus_tag="PKZ52_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNG58712.1"
                     /translation="MMRLSVGALALVVQGALLLGCSAFKPLNTQVPAQQAAALAAQVS
                     LPRQAQAKGDTYNWWVQFNDATLNQLMQDAWAHNNSLQIASSALDAARALYQNAQRDR
                     LPSTQMSASEIRRGLPQAQTPQGSARINTQVQAQFDSQWELDLFGRLRNQAAFAKAQA
                     SWAEADLAAVRSSISAEVAAVYVQLRSAQLQLRIAAENRQTQAQTFDLTKAYAQVGRS
                     DALDVARAEAQFQLTQANEQQFSAQVNTHINRLAVLTGNPMPALKEKLAAYQNLPNVP
                     AQFDLGDTQALIAARPDIRKAEQKARAALANYNVQVADLYPSIQLTGGLGFVAADWNS
                     LGNSGTDSFYFGPRLRWGAFDFGRVQARIKAADAQSQIALRQLDETLLTALEEIDNAM
                     VDFGQEELRRNGLQKAQAASLQAAKAAREKFTLGVGDLSTVLDVERSHLAVSAEYAQS
                     EARLLLDLIRIYKSLGTGRPGNQQDAVARSAMDEDSARKSQVLVF"
     gene            2603..3748
                     /locus_tag="PKZ52_02735"
     CDS             2603..3748
                     /locus_tag="PKZ52_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HNG58713.1"
                     /translation="MQQSRIDVLDALRGYALMGLFLIHMVEYYEIYWLNPIPHPLNDA
                     LFMVFGGKAFAIFAFLFGVSFYILLGNQQARGVDFRGRFIWRLSLLLAMGYVHSLLYG
                     GDILQVLALCGLCLVPLWRAPHFLVLCLSVFFLLQGPLWLECVALKLFPTAAAAPLNE
                     PDAVLKVYAEGSFAQLIITNAVLGNERKWAFMLESGRLWTVLGMSLLGFLLARLRVFS
                     NYAERARQFTIALFICVLAGALFFWVQLYIKEHNSGLGNLGAIASAYFNLAVTLGSVC
                     LFVLLYLSPLQKVLRYLAAPGRMTLSIYLAQSLVCVPIFYGFGLAAWAFLGQVNSFLL
                     GLLLWLLQIVLASLWFKHYRYGPMEWVWRAATFMRRDIPFRLKRTDG"
     gene            complement(4015..4875)
                     /locus_tag="PKZ52_02740"
     CDS             complement(4015..4875)
                     /locus_tag="PKZ52_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58714.1"
                     /translation="MSKPEPSHKDKHQLVAELEALHRSLSPEANIPVLVEAVLDSEEE
                     VPDLAVQIDLVDSLLAEQPAAKKAPAAELQLDLGYDLVDAADTPLPEDPNANESLSQV
                     HNDPLNYTGDDLFINHEDRESHFTQEPVDAEDQDEPPLLELEDALPSAEAVIPTTPDS
                     SAAKLDTVFKALSNPTDDQDDENPFLPAHLREKLRRSKATLIEEIQRSSDAVHQSSAL
                     IKAYTSDSQLKHRDRATTPIQPQKESLKEAEPNQALIDELVAEFLPQIEAKLRQKLAE
                     QLAHKPPRSN"
     gene            complement(4926..5345)
                     /locus_tag="PKZ52_02745"
     CDS             complement(4926..5345)
                     /locus_tag="PKZ52_02745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNG58715.1"
                     /translation="MSGRADFYVLPAGEERHHFACRLIEKAYKLGNKVWLRLDEQQLA
                     ALDDALWTFKPEAFVPHAAFGPDAKLEDCPVWLMTQLPDGDCDLLVNLASTPLEPPPS
                     AGRVVELVTQDAHILQLTRQQYARYKALNWVINTHKL"
     gene            complement(5347..6819)
                     /locus_tag="PKZ52_02750"
     CDS             complement(5347..6819)
                     /locus_tag="PKZ52_02750"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNG58716.1"
                     /translation="MELSSKPLPLQTSKTGCAIVAVFADKTFSDDYKRLDSTSQDTIN
                     RLIKAGDFTFKPGQMVWINTPAKAAYQRLLLVCLGASASELSLKDYTQSLQAVARTLA
                     TRPVGSAILYLNGIEGLSSHTLGFWAAEVFSKQAYRYTTTKKPKDGVHDLKSLHLSLP
                     KNQNKAANQGILEGEAVGHGINLARQLGNLPGNICTPTFLAQEAERLAKTDKKFSVKI
                     LDEKALSKLGTGSLLSVSRGSAEPPRLIVLEYKGKTSKQPHVLVGKGVTFDTGGISLK
                     PGAGMDEMKFDMCGAASVLGTLAALSQMQAAVHVVGIIAAVENMPGSRATKPGDVVTS
                     LSGQTIEILNTDAEGRLILCDALTYAERYKPKTVVDIATLTGACVVALGHHASGLFAN
                     NQALANALLSAGEAAQDRAWQLPLWDDYQKGLESNFADMANIGGREGGSITAACFLSR
                     FTKAYSWAHLDIAGTAWHSGGAKGATGRPVGLLVHYLLNA"
     gene            7028..8113
                     /gene="lptF"
                     /locus_tag="PKZ52_02755"
     CDS             7028..8113
                     /gene="lptF"
                     /locus_tag="PKZ52_02755"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNG58717.1"
                     /translation="MILFRYLAREMLQSMAAVSLVLLLVILSGRFVRYLAEAAAGKLD
                     AEVVFTLLSFRLPGYLELILPLGLFIAILLAYGRLYIDSEMTVLSACGVSEGTILRYS
                     LLCGLAVAALVAFFSLFLSPLGARAAEDLLAAQRNRTEFETLKPGRFHRLSNSAGVAY
                     VQEVDEGGKTLRQVFLAGKDQGSDALSVMTAKTGTTVMDDTSGKRYLVLGQGVQYRGS
                     PGQANYEEVEFATYQQYVPQPSMDAKPKKPSDMLSTLQLIKAADKEAKAALQWRLSMP
                     LLVLIVVALAVPLARTQPRSGRYVKLIPAIVLYVIYLVLANAARGAMEAGKSHLPYVL
                     WWVHGGFALLALVLFNPKRLMFWRAAQ"
     gene            8110..9171
                     /gene="lptG"
                     /locus_tag="PKZ52_02760"
     CDS             8110..9171
                     /gene="lptG"
                     /locus_tag="PKZ52_02760"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNG58718.1"
                     /translation="MTLLHNYIGRSVAGAIFIVLLVVLALQGISELVDQLGQMHGAYN
                     FSEVLVYVAFSLPAGIYDYLPLAALVGCLVGLGILASSSELVVMRAAGVSLLQILLSV
                     MRPVLGFIMLGVFLGEFVTPFTDQYGESRKALLLGRDSAITSGQGMWNREGNEYMHFA
                     ALLPNGDLIGVSRFEFSDEGRLKRASYAKTAEFQRGQWQERDGQFTELGTNQIQAGTF
                     DKRPWDSALNPTLLQVLMLGKDNLPMVRLYSYAQYLDEQGQDGKEFWLAFWKKALQPL
                     AITSLVMIAISFIFGPLRQVAMGSRVFGGVMVGIAFRTSQDLLGPSSLLFGFSPLFAV
                     LLPIALCAVIGLFLLRRGA"
     gene            9281..10084
                     /locus_tag="PKZ52_02765"
     CDS             9281..10084
                     /locus_tag="PKZ52_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter"
                     /protein_id="HNG58719.1"
                     /translation="MDQFSLLLSQGAAQAWWFLPTALGIGALHGLEPGHSKSLMAAFI
                     IAVRGTWQQAVLLGLCVAVSHSLVVWLIAAAGLYYGPAAQALLSEARLSQISGVLVIG
                     VALWMLWNLLRWRHSAHHTGCDHNHHDHDHDHDHDHDHHAHAEPKAALSAHDLAHARD
                     IERRFGSGQASLGQIVLFGLSGGLIPCPASIAVLALCLQAKQVVIGFALVAAFSLGLA
                     LTLMLSGLVAALGVKHASARFPFINRIGAYAPWLSVSLMLVLGSLLVMR"
     gene            complement(10285..11817)
                     /locus_tag="PKZ52_02770"
     CDS             complement(10285..11817)
                     /locus_tag="PKZ52_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNG58720.1"
                     /translation="MKQIKNTLVLGMLLGLAGCGGAAPNSDGSGSSSGAITSPGSSSS
                     SPSTGSTSGSTAPSSSGTPSSSGAPSSSGTPSSSGSPSTGSSSGSTTPSSSGLSGASG
                     TSSSSGGATASSSSGSSVPSSSSGSGTGAVQAGTLTAGDYDDVLNFTLYQDYISDYLQ
                     TASASVAVPYVDITQKIPVKILDASGAAAKGAKITLSTEEKVLASLRTPADGVVNIYP
                     VFDQLPASFQINITPPDGGAAITKTLLREQLTPGNKIEITLPTTVAANTNIDVTLVLD
                     TTNSMADEIKYLQAELAQVFNDIAADYAGASIRVGLVAYRDEGDIYVHKEFPFTEDLT
                     RFNADLASLFAGGGGDAPEAMDQGMNGALSLNWRENSTKIMLLVADAPPHNYEIAATW
                     KAALKAREQQIHIVPVGASGLGKRAEFLMRSMAALTHSRYIFLTDDSGVGNSHDEPSV
                     DCYVVTRLDASIRRVLASLIKGKREEPTSAEIIRTQGNYNQGVCEPAKPTSLPTQPAV
                     SE"
BASE COUNT         2461 a         3041 c         3613 g         3335 t
ORIGIN      
        1 gcggaaacct tacccgccgg tattgaattt gagtggacgg aattaaccta tcaggaagta
       61 ttagcgggta atacagccat ttatattttg cccttgtgtt tattgttagt gtttttagta
      121 ttggcggccc aatacgaaag tttggcgctg cctttggtag tagtttccat tgtgccctta
      181 tccattttgt ctgctttgtt tggtgtgtgg gtttccggtg gggacaataa tattttcacc
      241 caggtgagtt tgtttgtgtt ggcggggctg gctagtaaaa atgcgatttt aattggggaa
      301 tttgcccgcg aactagaaat acgcggtatg gccacctacc aagcggctat taccgccagc
      361 cgcatgcgtt tacggcccat tttaatgacg tctttcgcct ttattatggg tgtagtgcct
      421 atggtatttt ccaccggggc aggcgctgaa atgcgcagcg tgatgggaat tgctgttttt
      481 gctgggatgt tgggtgtgac tttcttcgga ctatttttta cccccatttt ttatgtctta
      541 gcgcgcaaac tggagggtaa aaaagctgcc gtccctgcgc ccttaatcaa ttcggagtat
      601 tccctatgat gcgtttatcc gtaggtgcgt tggctttagt tgtacagggc gcgttgctgt
      661 taggctgcag tgcatttaag ccattgaata cacaagtgcc cgcgcagcaa gccgccgcat
      721 tggccgcgca ggtgtcgctg cctcggcagg cccaggccaa aggcgatacc tataattggt
      781 gggtgcaatt taatgatgca accctaaacc aattgatgca ggacgcctgg gcgcacaata
      841 attctctgca aattgccagc agtgcattag atgccgcccg cgcactttat caaaatgcgc
      901 agcgggatcg tttgcccagc acccaaatgt ccgccagtga aattaggcgc ggcttgcccc
      961 aggcgcaaac accacagggc agtgcacgca ttaatactca agtgcaggcg cagtttgata
     1021 gccagtggga gttggattta tttggtcggc tgcgtaatca agcggcgttt gccaaggcgc
     1081 aagcaagctg ggctgaagcg gatttagccg ctgtgcgcag cagtattagt gcagaagttg
     1141 ccgccgtgta tgtgcaattg cgcagcgcgc aattacaact gcgcatagcg gctgaaaatc
     1201 gccaaaccca agcgcagacc tttgatctca ccaaagccta tgctcaagtg gggcgcagcg
     1261 atgctttgga tgtggcccgc gctgaagcgc aatttcaatt aactcaagcc aatgaacaac
     1321 aatttagtgc tcaggtgaat acccatatta atcgcttggc ggtattaacc ggtaacccca
     1381 tgcccgcact taaagaaaaa ttagcggctt atcaaaattt acccaatgta cctgcgcaat
     1441 ttgatttagg tgatacccag gccttaattg ctgcgcggcc ggatattcgc aaagcggagc
     1501 aaaaagcccg tgcggcgctg gccaattata atgtgcaggt ggcggactta tacccgagta
     1561 ttcaattaac cggcggtttg ggttttgtgg cggccgattg gaatagcctg ggtaattccg
     1621 gtacggatag cttctatttt ggtcctcgtt tgcgctgggg tgcatttgat tttggccgtg
     1681 tgcaggcgcg tataaaagct gcggatgcac agagtcaaat tgctttgcgt caattggatg
     1741 aaaccctgct caccgcatta gaagagattg acaatgccat ggtggatttt ggccaggaag
     1801 agctgcgccg caacggtttg caaaaagctc aagcggcgag tttacaggct gcgaaagccg
     1861 cgcgagaaaa attcactttg ggggtagggg atttgtccac tgtgttggat gtggagcgca
     1921 gccatttggc cgtgagtgcc gaatatgcgc aaagtgaagc gcgtttatta ttggatttaa
     1981 ttcgtattta taaaagtttg ggtacgggaa gaccgggcaa tcagcaggat gctgtggcgc
     2041 gctcggctat ggatgaagac tctgcgcgca agagccaggt attggttttt taacgccggg
     2101 gtgacgggag tgagtttctc gttattcgtt tctcattttg ggctctgcgt gggaacgagg
     2161 aatcccaccc cagccctccc cttgaaaagg ggagggggcc aaaacgcctt gtgcactagc
     2221 acactgcctt ggtttgaggg ttttttgctt ctattctttt tgctgctctc cttttgggtt
     2281 agcgcctagc ctaatccgcg ggcgtgcaca aatcccggat tacgccagtc tccatcctgg
     2341 cccccagata tttcctcttc agcttttcgc ctctgttact taacttaatg atattgcttg
     2401 aaaaggcctg gctgcgaggg tgccgtattt ttattaagca actgcttatc cagcgccggt
     2461 tgtttattgc ttaataacgc ggccaagtct catttttagc tgcacgctgg cctgacgatt
     2521 caaccgccca gggctataat tttgccgata ccctaactgt gaaccggttc ggcttgggcc
     2581 gatttttggc ggacaattta ctatgcaaca atcgcgaatt gatgtgttag atgcgctgcg
     2641 cggctacgcg ctgatgggtt tatttttaat ccatatggtg gagtattatg aaatttattg
     2701 gttaaaccct ataccccatc cgctcaacga tgcgctgttt atggtatttg gcggcaaggc
     2761 gtttgctatt tttgcgtttt tatttggtgt gagtttttat attttgcttg gcaaccagca
     2821 ggcgcgcggg gtggactttc gcggtcggtt tatctggcgg ttgagtttat tattggcgat
     2881 gggctatgta cacagcttgt tatacggcgg tgatatttta caagtgctgg ccctgtgcgg
     2941 cctatgttta gtgcccctgt ggcgcgcgcc acattttttg gtcctgtgtt tgagtgtgtt
     3001 ttttctgttg caggggccgc tgtggttaga atgtgttgcg ctgaaattat tccccaccgc
     3061 tgcggccgcg ccgcttaatg agccggatgc ggtattaaaa gtctatgccg aaggcagttt
     3121 tgcacaatta attatcacca atgccgtgtt gggtaatgag cgcaagtggg cgtttatgct
     3181 ggagagtggg cgcttgtgga cggtgctggg catgagcctg ttgggttttt tactggcgcg
     3241 gctgcgggta ttttctaatt acgctgagcg tgcgcggcaa ttcaccattg ctttatttat
     3301 ctgtgtgctg gctggggcgc tgtttttttg ggtgcagttg tatattaagg agcacaatag
     3361 tggccttggc aatttaggcg ctattgccag tgcgtatttt aatctggcgg taaccctggg
     3421 cagtgtttgt ttatttgtgt tgttgtatct gtcgccactg caaaaagtgt tgcgttattt
     3481 agcggcgccg gggcgtatga cgttgagtat ttatctggcg cagtcgcttg tatgtgtgcc
     3541 gattttttac ggctttggtt tggcggcttg ggcgttttta ggccaggtga atagtttttt
     3601 attgggcctg ctgttgtggc tgctgcaaat agtcttagcc agcctttggt ttaaacatta
     3661 ccgctatggg cctatggagt gggtgtggcg cgcggcgacg tttatgcgcc gggatattcc
     3721 gtttaggtta aagcgcactg acgggtaata agttggcaat aaaaatggca ataaatggcg
     3781 actgtattag cggaatttta ggtttaggag ccactgatta acctgtatct tcaagaatac
     3841 ggaccgacct gaggttcctt taacggatag acccggggta tctttaacat atcgacctga
     3901 ggtctcttta acagaccaac cgggggtttc tttaacggca gcgctagtta ctcagtaaat
     3961 gattgttctg gcacctatgc cgtacgcggc gccgcaagct gcgttacccg gtgattaatt
     4021 agagcggggc ggtttatgcg ctagttgctc cgctaatttt tgccgcagtt tcgcttcaat
     4081 ctgcggtaaa aactctgcta ccaattcatc aatcagggcc tggttgggtt cggcttcttt
     4141 aagtgattct ttttgcggtt gtattggcgt tgtggcgcgg tcccgatgtt ttaattggct
     4201 gtcgctggtg taggccttaa ttagcgccga gctttggtgt acggcatcgc tggagcgctg
     4261 aatttcttca attaaggtag ctttactgcg gcgcaatttt tcccgcaggt gcgccggtaa
     4321 aaacgggttt tcatcgtcct ggtcgtcggt gggattactg agggctttaa aaacggtatc
     4381 cagcttagcg gctgaggagt cgggtgttgt gggaatcacc gcctcggctg agggcagggc
     4441 gtcttccagt tccaacagcg gcggctcatc ctgatcttca gcgtccaccg gttcctgggt
     4501 gaagtggctt tcgcggtctt cgtggttaat aaataaatca tcgccggtgt aattaagcgg
     4561 gtcgttgtgc acctggctta agctttcgtt ggcattgggg tcttctggca ggggggtatc
     4621 ggcggcgtcg accaggtcgt agcctaaatc cagttgcagc tcggccgctg gtgccttttt
     4681 cgccgctggt tgttcggcaa gcagggaatc gactaagtct atttgcaccg ccaagtccgg
     4741 cacttcctct tcactgtcca acaccgcctc caccagcacg gggatattgg cctctgggct
     4801 gaggcttctg tgcagggctt ccagctcggc aactagctgg tgtttgtctt tgtggctggg
     4861 ttctggtttt gacataagcg tcgctacagt gagagaacgt ggtttaagga acctggttta
     4921 aggcgctaaa gcttgtgggt gttaataacc cagtttagcg ctttatagcg ggcatattgc
     4981 tggcgtgtca gctgcaatat gtgcgcatcc tgggtgacca gttcaactac ccggcccgca
     5041 ctaggcggcg gctccagcgg tgtagaggcg agattcacca acaggtcaca gtcgccatct
     5101 ggcagttggg tcatcagcca gacggggcag tcctctagct tggcgtctgg cccaaaggcg
     5161 gcgtggggta caaaggcttc cggtttgaag gtccagagag cgtcatcaag ggccgcaagc
     5221 tgttgctcat ccaggcgcag ccacaccttg ttgcctaatt tataggcctt ttcaattaac
     5281 cggcaggcga agtggtggcg ctcttcgcct gcgggcagca cataaaagtc ggcgcggccg
     5341 ctcatcttag gcgttgagta ggtagtgcac caacagcccc accgggcggc cggtggctcc
     5401 tttggcgccg ccggagtgcc aggcggtgcc ggctatatcc aggtgggccc agctgtaggc
     5461 tttggtaaaa cggcttaaaa agcaggcggc ggtgatggag ccgccctcgc ggccgccgat
     5521 attggccata tcggcaaagt tgctctccag gcctttttgg taatcatccc aaaggggcag
     5581 ttgccaagcg cgatcctggg cggcttcgcc cgcactgagt agcgcgttgg cgagggcttg
     5641 gttgttggca aacaggccgc tggcgtgatg gcccaaggcc accacacagg cgccggtaag
     5701 agtggctata tccaccacgg ttttgggttt gtagcgctcg gcgtaggtga gggcatcgca
     5761 gaggatcagg cggccttcgg catcggtgtt gagaatttca atggtttggc cggacaggga
     5821 agtgaccaca tcgccaggtt tggtggcgcg gctgccgggc atgttttcta cagcggcgat
     5881 aatacccacc acatgcacgg cggcctgcat ctgggagagg gccgccaggg tgcccagcac
     5941 cgaggcggcg ccgcacatgt caaatttcat ttcatccatg cccgcgccgg gttttaggct
     6001 gatgccaccg gtatcaaagg ttacgccctt gcccaccagt acatggggct gtttgctggt
     6061 tttgcctttg tattcaagca caatcaggcg cggcggttcg gcggagccgc gggataccga
     6121 caacagcgag ccggtaccga gtttgctgag ggccttttca tcaaggatct tcacgctgaa
     6181 ttttttatcg gttttggcca gacgctcagc ctcttgggca agaaacgtcg gcgtgcagat
     6241 attaccgggc agattgccca gctggcgggc gaggttaatg ccatggccca ctgcttcacc
     6301 ctcaagaatg ccttggtttg ccgccttatt ttggtttttg ggcaggctta agtgcaggga
     6361 tttaaggtcg tgcacgccgt ctttaggttt tttggtggtg gtgtagcggt aggcctgttt
     6421 gctaaatact tcggcggccc aaaaacccag ggtgtggctg ctcaggcctt ctatgccgtt
     6481 taaatacaat atggctgagc ccaccgggcg ggtagcgagg gtgcgggcaa cggcctgcag
     6541 gctttgggtg tagtccttca ggcttaattc gctggcactg gcgcctaggc agactaacaa
     6601 cagccgttgg taggcggctt tggcgggagt gttgatccag accatttggc cgggtttgaa
     6661 ggtgaaatca ccggctttaa tcaggcggtt gatggtgtct tggctggtgc tatcgaggcg
     6721 tttgtaatcg tcgctgaagg ttttatcggc aaaaaccgcc acaatggcgc aaccggtttt
     6781 gctagtttgc aacggcaatg gcttagagga tagctccata aatcgggcac ctgagagttg
     6841 gaagtgggtg ggtagcgggt agactttaag tgggccttgc caccataatc gccggcctgt
     6901 gtagccgctc agtgtagcct attggctcag ccttaggcac atcctggcag cggtgatttc
     6961 ctctctttgc ctgctagccg ttgtaggtgc agcatcaaag gtcccttcgg ttaactaggt
     7021 tcctgctgtg attttgtttc gttacctcgc ccgcgaaatg ctgcagagca tggcggcggt
     7081 gagcttggtg ttgttattgg tgattttaag tggccgcttt gtgcgctacc tagcggaggc
     7141 cgcagcgggc aaactggatg ccgaggtggt gttcaccctg ctgagctttc gcttgcctgg
     7201 ctatttagag cttattctgc cgctgggttt attcatcgct attttgctgg cctatggccg
     7261 cctgtatatc gatagcgaaa tgaccgtgtt gtccgcctgt ggcgtaagcg aaggcaccat
     7321 tttgcgctac agcctgctgt gtggtttggc ggtggcagcc ctggtggcat ttttctcgtt
     7381 atttttaagc cctttgggtg cgcgggcggc ggaggactta ctggccgccc agcgcaatcg
     7441 cacggaattt gaaaccctca agcccggccg cttccaccgc ttaagcaaca gtgccggtgt
     7501 ggcctatgtg caggaggtgg acgagggcgg taaaaccctg cggcaggtat ttttagccgg
     7561 taaagaccag ggcagcgatg cgctttcggt gatgaccgcc aagaccggca ccacggtgat
     7621 ggatgacacc agcggcaagc gctatttggt gctgggccag ggggtgcagt atcgcggctc
     7681 gccgggccag gccaattatg aggaggtgga gtttgccacc taccagcagt atgtgccgca
     7741 gccctccatg gatgccaaac cgaaaaaacc cagcgatatg ctctccaccc tacaactaat
     7801 aaaagctgct gataaagaag ctaaggctgc cttgcagtgg cgcctctcca tgcctttatt
     7861 agtgctgatt gtggtggccc tggcggtgcc tttggcgcgc acccagccgc gctccggccg
     7921 ctacgtcaag ttaattcccg ccattgtgtt gtatgtcatt tacctggtgc tcgccaatgc
     7981 cgctcgcggt gctatggagg cgggtaagag ccatttgccc tatgtgctct ggtgggtgca
     8041 cggcggtttt gcgctactgg ccttagtgtt attcaaccct aaacgcctga tgttttggag
     8101 ggcggcccaa tgacactgct gcacaactac ataggccgca gtgtggccgg ggcgattttt
     8161 atcgtgctgc tggtggtgct ggctctgcag ggtatatcgg aattggtaga ccagttaggc
     8221 cagatgcacg gcgcctataa tttttctgaa gtgctggtat atgtggcctt tagcctgccc
     8281 gccggtattt acgactacct gcccctggcg gctttggtgg gctgtttggt ggggctgggg
     8341 atcctcgcca gcagcagcga actggtggtg atgcgggcgg ccggggtgtc cttgctgcaa
     8401 attctgctct cggtgatgcg cccggtgctg ggctttatta tgctcggggt gtttctgggc
     8461 gaatttgtta ctccttttac cgaccagtat ggcgagtccc gcaaggccct attattgggg
     8521 cgggattcag ctattacctc cggccaaggc atgtggaacc gggagggcaa tgaatatatg
     8581 cactttgccg ccctgctgcc caatggcgat ttgatcggcg tgagccgatt tgagtttagc
     8641 gacgagggcc gcttaaaacg cgccagctac gctaaaaccg ccgagttcca gcgcggccaa
     8701 tggcaggagc gggacggcca gtttaccgag ttgggcacca atcaaattca agcgggcacc
     8761 tttgataaac gcccctggga ctccgctctc aaccccactc tgttgcaggt attaatgctg
     8821 ggcaaagaca acctgcccat ggtgcggctg tattcctacg cccagtacct ggacgagcaa
     8881 ggccaggacg gcaaggaatt ttggctggcg ttttggaaaa aagccctcca acccctggcc
     8941 atcaccagcc tagtgatgat cgctatttcc tttattttcg gccccctacg gcaggtggcc
     9001 atgggctcgc gggtgtttgg cggcgtgatg gtggggattg cctttcgcac cagccaggat
     9061 ctgctcggcc ccagcagttt gctgtttggc ttctcgcctt tatttgctgt gttgctgccc
     9121 attgccttgt gtgcggtgat cggtttgttc ctcttgcggc ggggcgccta aatcttgctg
     9181 cggcgcatgg cttaagaggc ttattgtttt aaggctgagc taatgcagtg gggttacgct
     9241 tgccggtcta tcaatcatta gccaattgcc ctgaggtgtt atggatcagt tcagcctgtt
     9301 attaagccag ggcgctgccc aagcctggtg gtttttgccc accgccttag ggataggcgc
     9361 cttgcacggg ctggaacccg gccactccaa atccctgatg gcggcattta ttatcgccgt
     9421 gcgcggcacc tggcagcagg ccgtgctgct ggggctgtgt gtggcggtat cccacagcct
     9481 agtggtgtgg ttgattgccg cagcgggcct ctattacggc ccagcggccc aggctttgtt
     9541 gagcgaggca cggctcagcc aaataagcgg cgtcttggta attggggtgg cgctgtggat
     9601 gctgtggaat ttactgcgct ggcggcacag tgcacaccat accggttgcg atcataatca
     9661 tcacgaccac gaccacgacc acgaccacga ccacgaccat catgcccatg ctgagcctaa
     9721 ggccgcgctg tcggcccatg acttggccca tgcgcgggat atagagcgcc gctttggcag
     9781 cggccaggca agccttgggc aaattgtctt gttcggcctc agtggaggcc ttattccctg
     9841 cccagcttca attgctgtat tggccctctg cctgcaggct aaacaggtag ttataggttt
     9901 tgcactggtg gcggcgttta gtttggggct ggcactcacg ctgatgctga gcggcttggt
     9961 ggcggcctta ggggttaaac acgccagtgc gcgttttcct tttattaacc gcatcggcgc
    10021 ttatgcgcct tggttgtcgg taagcctgat gttggtgttg ggtagtttgt tggtaatgcg
    10081 ctgaattatt aaataaatcg tcctgatcta ttttctcgtt gttccgctga cacttaccaa
    10141 aatgcgggga ttttttgttt tagcgatgct agtggcagct tgtgccctca atgctgcttt
    10201 cttgctgcca ttattaaccc ggcataaaaa gctgccgagt taaaaaaaat accgggctta
    10261 agcccggtat ttttttgccg gtaattattc acttacggca ggttgtgttg ggaggctggt
    10321 gggtttagcc ggttcacaca cgccttggtt gtaattgcct tgggtgcgaa taatttcggc
    10381 gctggtaggc tcttcgcgct tgcctttaat tagactggcc agcacgcggc gaatcgatgc
    10441 atccaggcgc gtcaccacat aacaatccac actgggttca tcgtggctat tacccacgcc
    10501 cgagtcatcg gttaaaaaga tatagcggct gtgggtgagg gcggccatgg agcgcattaa
    10561 aaattccgcc cgtttgccta aaccactggc acctaccggc actatatgaa tttgttgctc
    10621 gcgggccttt aaggcggcct tccaggtggc ggcaatttcg taattatggg gcggcgcatc
    10681 cgctaccagc agcattattt tagtgctgtt ttcccgccaa ttaaggctca gcgctccgtt
    10741 catgccttgg tccatggcct ccggcgcatc gccaccacca ccggcaaata ggctggctaa
    10801 atcggcatta aaacgcgtta aatcttcagt aaaaggaaat tctttgtgca cataaatatc
    10861 gccttcatcg cgataggcca ctaagcctac gcgaatggag gcgccagcat aatccgctgc
    10921 aatatcatta aatacctggg ctaattctgc ctgtagatat ttaatttcat cggccatgct
    10981 attggtggtg tccagcacca gcgtcacatc aatattggtg ttagctgcaa ctgtagtggg
    11041 cagggttatt tctattttat tgccaggcgt taattgctcg cgcagcagtg ttttagtgat
    11101 cgccgcgcca ccgtccggtg gggtgatatt tatttgaaac gaggcgggta attgatcaaa
    11161 caccggataa atattcacta ccccatcggc aggtgtgcgc aggctggcta acactttttc
    11221 ctcagtgctt agggttattt tggcaccctt ggctgctgcg ccgctggcat cgagaatttt
    11281 cactggaatt ttttgtgtga tatccacata aggaaccgca acactagcac tggcggtttg
    11341 cagataatca ctgatgtaat cctgatacag agtgaaattg agtacatcgt cataatctcc
    11401 tgcggttaag gtaccggctt gaactgcacc cgtgcctgag ccggatgaac tgctgggtac
    11461 actgctaccg ctactgctgc tggcggtggc gccgccggag cttgagctgg tgccacttgc
    11521 accgcttaag ccactgctgg aaggcgtggt tgagccggag gaagagccag tactgggcga
    11581 accactactg ctgggagtac cgctactgct aggagcaccg ctgctgctgg gtgtaccact
    11641 gctgctgggt gctgtggagc ccgaggtgga acccgtggag ggcgagctgg aacttgaacc
    11701 ggggctagtg atagcgccgg aactggatcc gctgccatcg ctattggggg ctgcgccgcc
    11761 gcagcctgcc aatcccaata acatacctaa cactaaggta ttttttattt gcttcataat
    11821 ctgctccgtt gtgagtgccg ttcatctggc cacaacggta ttcaatttaa cgctgaaaat
    11881 atgtacgtaa atttacgtat ccagataatt ctgtgaccca tgtccgcatg gtttttagga
    11941 ttactaaccc ggcggcaaaa tagattgtcc tgatccattt tacctgccct gtgctgggtg
    12001 catcgaacac gccaataaat ttactcaaaa accccgtggc gcatcggtta atacgtattc
    12061 tacgcgccga aggcggattt ttggcgttat tcaaacttag gccccccacg ctcgcccagt
    12121 gcggcggatt attgcggtta aaacggataa atagccatgt ggccgtgtga attggtctat
    12181 aactaaaggt ggcaaaccgc acaggggcgc aggtacttga tcgcttcaag ttaatcctag
    12241 ttgctgcaag tcagccgcgt taagttaacc gcctcagaat aggcgcctct tgcacagcct
    12301 ttggttgcgg aatatgttca aaaaccctca agccgtttta gggttcaagc agcaggctac
    12361 ctgcccgcga cggttttgaa aatagccatt caatacgggc tgcaaaggtg ttattgggaa
    12421 ataaaatgat ggaaacgtgc tgcaagcaat
//
LOCUS       DAOQGS010000043        12273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2394_length_12273_cov_4.249386, whole genome shotgun sequence.
ACCESSION   DAOQGS010000043 DAOQGS010000000
VERSION     DAOQGS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_12273_cov_4.249386"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1487)
                     /locus_tag="PKZ52_02775"
     CDS             complement(105..1487)
                     /locus_tag="PKZ52_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNG58721.1"
                     /translation="MTALHALIIDDEPDICELLQLTLARMQVSADTATTVKEAKALLV
                     SRDYQLCLTDMKLPDGNGIELVRWINQQQLNLPVAVITAHGNTDAAVEAMKAGAFDFI
                     SKPVELSTLRKLVASALQTHQLPGGETQPAGLFGRSACMQQLENSVRKLARSQAPVYI
                     FGESGSGKELAARFIHQLGPRANKPFVPVNCGAIPKELMESEFFGHKKGSFTGAHVDK
                     TGLFKAADGGTLFLDEVADLPLDMQVKLLRAIQEKAIRVVGGSQEETIDVRILSATHK
                     NLEDEVSAGNFRQDLFYRLNVIQLKVPPLRERPEDIADLCQLFLANIAKEMQLPAPKL
                     SKDALKMLLAYEFPGNVRELENILERAFTLCEGDSIEAADLHLQTSHTPPKIRKQTER
                     ASAGNTDHFARALEFPTLDEYLAEIEKEVLMGALEKARWNKTLAAKELNITFRSLRYR
                     LQKLGLDDDA"
     gene            complement(1490..3070)
                     /locus_tag="PKZ52_02780"
     CDS             complement(1490..3070)
                     /locus_tag="PKZ52_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG58722.1"
                     /translation="MAPHAIAPLTHSKPLLNIYYAFRIGLAFILLLLFLSGRTGRALG
                     GLYPDVFFWTCATWIIVATLSLAFFPAQKLLHSLHRITTFLVLDVIALLLLIHASGGV
                     DSGFGYLLLVIVAISSIFMRQRMAMMFATMVSLLMIAQTLYLVESLNLGGKPIFSAGV
                     LGILLFITAFTFQRLTQKIQLSNEQAIQQRLYAEHLQRLAQAIVARMRTGIIVLDNDN
                     KIELMNTAASQLLGANINAQLDDNPALSQLKKYLSAEDEPGLRQQRSEAGLEVRFSRA
                     HLNVGDLKHQVVFVEDTRTIAQQAQQLKLASLGRLTASIAHEIRNPLGAISHAVQLLQ
                     ESDAITNMDKRLCDIIYQHAKRVNQIIENTLALSRRKDPQTSLIDLHPWLEQFINNYS
                     SHQQANITLDLEPGPLPLRIDTTNLQQILVNLVDNGLRYSKQQTGEAKLLLRGGINQS
                     NDNVFLEVIDFGPGIDGEQLAQIFEPFYTTEELGTGLGLYICKELCEINQASLYYFKT
                     SRGESCFRIDFPHHLRMI"
     gene            3470..5077
                     /locus_tag="PKZ52_02785"
     CDS             3470..5077
                     /locus_tag="PKZ52_02785"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNG58723.1"
                     /translation="MSMFTAAMAQINPWVGDIAGNCSKIIAVSRSVAEQGAADWVIFP
                     ELTLTGYPPEDLLLRPALKPRIEAALARLCAELPPELAVVVGYPKVTADGVFNAAGIL
                     YQGQIQLEYCKQCLPNYQVFDEKRYFTAGAAMPVWQHKGSCVALSICEDIWHPGPAAQ
                     AKAAGAQVLINLNASPFHRGKQRTREDVVAERCAQTGLAVVYVNQVGGQDELVFDGGS
                     FAMNSQGQLLARADYCVEQTVLVNLGAPGSIAAPPSDLASVYSALVLGVRDYVRKNRF
                     KGAVLGLSGGIDSALTLAIAADALGPEQVDAVMMPFRYTSDMSQTDAALQASLMGVRY
                     QSISIEPIFSAFEAALKPAFHNAAADKTEENLQARSRGVILMALSNKTGSLVLTTGNK
                     SEMAVGYATLYGDMAGGLDVLKDVPKTLVFALARYRNSLNPVIPERVITRPPSAELAP
                     GQKDEDSLPPYEVLDQILALYIEEDASAADIIKRGFAAETVKKVLRMVDLNEYKRRQA
                     AIGIRISQRGFGRDRRYPITNGWALDD"
     gene            5120..6346
                     /gene="uxuA"
                     /locus_tag="PKZ52_02790"
     CDS             5120..6346
                     /gene="uxuA"
                     /locus_tag="PKZ52_02790"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNG58724.1"
                     /translation="MHETWRWFGPKDPITLEKIRQAGAKGIVTSLHHIPTGEAWPLED
                     VLARKQQIEAAGLEWSVVESIPLHNEIKLRTGNFRQLIKQYQQSIQAVGEAGVKTVCY
                     NFMPVVDWTRTNLNYRLDNNAEALRFEMTDFAAYDVYMLQRQGAAGSYSPEVLSRAKS
                     RFDAMSAQERALLERNIIAGLPGGEGSYNRDGVRAAIEEFIALGEGQFRANLIAFLQE
                     IIPVAEEYGVNMCVHPDDPPFSLFGLPRVVSTAADVRAIFTAVPSRHNGLTLCAGSFG
                     ARGDNDLVAIAREFGPVVHFVHLRNVKREEDGSFFEAEHLDGDNDMVGLISALLDEQA
                     RRKAMGDANWQIPMRPDHGHLLADEIGQPGVNPGYSYVGRLKGLAELRGVIVALEAVR
                     KTPGVYAPQVFGPPSN"
     gene            6836..7597
                     /locus_tag="PKZ52_02795"
     CDS             6836..7597
                     /locus_tag="PKZ52_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNG58725.1"
                     /translation="MKVKSAKPKNFNAQVVEYIGSRILHGQYNEGEKLPVESELSREL
                     GVSRPILREATKILSSKGLLRAKPKVGTIVTERKHWNMLDSDVLGWLIESKPPVEFLD
                     MLFDARSAIEPRAAELAALKATEADLEDIRLAYEDMARVESIYESFEPDIRFHQAILA
                     ATHNEVVMYIGHTLHNALAISFSLTTWDKELYMVAVAKHKAIYDAIAERNPQKAGDAV
                     RHLLMASREDFNLRQAEEGPVPALSELDGLFSEVE"
     gene            7774..8544
                     /locus_tag="PKZ52_02800"
     CDS             7774..8544
                     /locus_tag="PKZ52_02800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG58726.1"
                     /translation="MNLTNRYPSLAGTLVLITGGATGIGAGMVEAFCLQRARVVFLDI
                     LQPEAEQLVNKVAALDGATAPEFFYCDLADIPALQAVIASIEQRFGPIGVLINNAARD
                     TRHDYSTVSAAYWDEMLSVNLRHVYFAIQAVSAGMKSLGGGSIINFGSVSWYRCQANL
                     TCYATSKAALEGMTRSFARDLGVDGIRVNTLIPGWVMTERQLRDWVNAETLSEIQKAQ
                     CLKPNVQVEDVCALALFLASNDSKMCTAQNFIVDGGWI"
     gene            9613..12198
                     /locus_tag="PKZ52_02805"
     CDS             9613..12198
                     /locus_tag="PKZ52_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNG58727.1"
                     /translation="MVLRSQFSVLCCAVLAVACAQTSVKPQGALHPGGYFAAPGVNVF
                     AFNSPPGGLFFDAKTSGVEIIHHGERTATNGDVRMFATPEQWDGMGQLVSRKVDEQAK
                     TITTQLAYPELGFNYSVVVAAYGQGYKVSVYLDQPLPQSLVNKAGFNMEFLPSVYWDK
                     AYLMDTKAGQFPRAASNANPMLMSADGKTEPSPLAVGKHLVLAPEDAQQRVAIHEAIG
                     DNTLMLFDGRRKAQNGWYVVRSMIPQGKTGKVIEWDIDIKSTPNWIREPVIGFSQVGY
                     HPAQTKRAIIELDEHDKATPSITLLRINTDGSKTPVQKAVATPWGNYLRYQYAVFDFT
                     QIKEPGLYQLQLGAQTTPAFPIGNEVLANSWQPTLDFFMPVQMDHMRIKEAYRTWHGL
                     SHMDDALQAPPNYNHFDLYAHNAQLDTQYKPFEHIPGLNIGGWYDAGDFDIRTQSQYE
                     TVVGLVHAFEEFSIPRDNTTISQAKRYVQINRPDGVPDILQQIEHGTLALIAQHRALG
                     HAIPGIVEGRLYQYPHLGDGASKTDNKIYNKALDPHAASRKVGENYEPSTVDPTPFEA
                     LMPENGLESGRFDDRWAFTSNTSPLNYGSAAALAAASRVLKTINPALAQECLTTALKV
                     WQTEAKRSPNTYHVGNTTGGKLEDEQLSAAVELLLTTRDSQYATAIRTLLPSVRERFY
                     GGNVELLVLAAPFMDAEFNQTLRSLAETYVTRAKQMTSENPYGVPITRGSWAGNGVVM
                     GFGIANFWLHKKFPDLVDADWVYRPMSYIVGTHPDSNYSFVSGVGAQSQTKAYGNNRA
                     DFSFIPGGVVPGVLVLAPDYPENKSDWPFFWGQNEYVIPMASHYIFLSHAAAALAAAQ
                     EKATP"
BASE COUNT         2946 a         2869 c         3281 g         3177 t
ORIGIN      
        1 aggatgtatt cacggcgtgt ctgaaatgat gtgtattcat tgccggtgcc taggtggtaa
       61 cccggcggca tttagtggcg ggttaatagc attgcaacac gctgttaggc atcatcatcc
      121 aagcccagtt tttgtaagcg gtagcgcaag gagcgaaagg tgatatttaa ttccttggct
      181 gccagagttt tattccagcg ggctttttcc agcgccccca tcaaaacttc tttttcaatt
      241 tccgctaaat attcatctag ggtaggaaac tccagcgcgc gggcgaaatg atcggtgttt
      301 cccgccgatg cgcgttcagt ctgtttgcgg attttagggg gtgtatggct ggtttgtaag
      361 tgtaagtcgg cggcttcgat actgtcgcct tcacataggg tgaaggcgcg ttctaaaata
      421 ttttcaagtt cccgcacatt gccggggaac tcataagcca ataacatttt taaggcatct
      481 ttagataatt taggggcggg caattgcatt tctttggcga tattggctaa aaaaagctga
      541 cataaatcgg cgatatcctc gggccgctcc cgcagtggcg gcacttttag ttgaatgacg
      601 tttaagcggt aaaaaagatc ctgccggaaa ttgcctgccg atacttcatc ctctaggttt
      661 ttatgggtgg ccgataaaat gcgcacgtcg atggtctcct cttgggagcc gcccaccact
      721 cgaatggctt tttcctgaat agcgcgcagc agtttaactt gcatatccag gggtaaatcg
      781 gccacctcgt ctaaaaataa agtgccgcca tccgccgctt taaataaacc ggttttatcc
      841 acatgggcgc cggtaaagga gccttttttg tggccaaaaa attcactttc catgagttct
      901 ttgggaatgg cgccgcagtt aacgggcaca aaaggtttgt tggctcgcgg gccgagttga
      961 tgaataaacc gtgcggccaa ttctttaccg ctgccggatt cgccaaatat ataaaccggt
     1021 gcctgggagc gggctaattt gcgcacgctg ttttccagtt gttgcataca ggcggagcgg
     1081 ccaaatagac cggccggctg agtttcgccg ccaggtaact ggtgggtttg cagggcgctg
     1141 gcgacgagtt tgcgcagtgt acttaattcc accggtttgg aaataaaatc aaatgcaccg
     1201 gctttcatgg cttccacggc agcgtctgtg ttgccgtggg cggtgatcac ggccaccggt
     1261 aaattcaatt gttgctgatt aatccagcgc actaattcga tgccattgcc gtcgggcaat
     1321 ttcatatcgg tcaggcataa ttgataatcg cggctcacca atagcgcttt agcttctttt
     1381 acggttgtcg cggtatcggc gctaacctgc atacgcgcta aggtgagttg cagtaattca
     1441 caaatatccg gttcgtcgtc aataataaga gcgtgcagtg cagtcataat taaatcatgc
     1501 gtaaatgatg ggggaagtcg atgcgaaagc aactctcgcc acggctggtt ttaaaataat
     1561 aaaggctggc ttggttaatt tcacataact ctttacagat gtataaaccc agcccggtac
     1621 ccagttcttc cgtagtgtaa aagggttcaa aaatttgtgc taattgttcg ccgtctatac
     1681 cggggccgaa gtctatcacc tctaaaaaaa cattgtcatt gctctgatta atgccgccgc
     1741 gcagcagcaa ttttgcctca cctgtttgct gtttgctata gcgcagcccg ttatccacta
     1801 aattcactaa tatctgctgc aaattagtgg tgtcgatgcg caggggcaag gggccgggct
     1861 ctaaatctag ggtgatattg gcctgttggt ggctggagta attgttgata aattgctcaa
     1921 gccaggggtg taaatcgatc aggcttgttt gcggatcttt gcggcgggag agggcgaggg
     1981 tgttttcaat aatttggttt acccgtttgg cgtgttggta aattatgtcg cacaggcgtt
     2041 tatccatgtt tgtgatggcg tctgattctt gtagcaattg cactgcgtgt gaaatggccc
     2101 ccaaggggtt gcgaatttcg tgggcaatgc tggctgtgag gcggcctaag gaggctaatt
     2161 taagttgctg ggcctgttgg gcgatggtgc gggtatcttc aacgaatacc acctgatgtt
     2221 taagatcgcc gacatttaaa tgcgcgcggc taaagcgcac ttcaaggcca gcctcgctgc
     2281 gctgctgacg taaaccaggc tcgtcctctg cgcttaggta ttttttcagc tggctcaacg
     2341 ccggattatc atcgagctgg gcattaatat tggcgccgag caattgggat gcggcggtat
     2401 tcattagttc aattttattg tcgttatcta gcacaataat gccggtgcgc atgcgtgcca
     2461 ctatggcttg ggccagccgt tgtaagtgtt cggcgtatag gcgctgttga atcgcttgct
     2521 cgttgctaag ttgaattttt tgtgtgaggc gctgaaatgt aaaagcggtg ataaataata
     2581 aaatgcctaa tacaccggca gaaaaaatcg gtttgccacc cagatttaaa ctctccacca
     2641 aatacagtgt ttgcgcaatc atcagcaggc ttaccatggt ggcaaacatc atcgccatgc
     2701 gctggcgcat aaaaatgctg ctgatggcga caatgaccaa taataaatag ccaaaacctg
     2761 agtcaacgcc gccgctggcg tgaattaata agagtagggc aataacatcc aatactaaaa
     2821 aagtggtgat gcggtgcagg gagtgcagca gtttttgggc gggaaaaaag gccaggctta
     2881 gggttgcaac tataatccag gtggcgcagg tccagaaaaa aacatctgga tataacccgc
     2941 ctaaggcccg cccggtgcgg ccacttaaaa ataacagcaa taaaataaac gccaggccaa
     3001 tgcgaaaggc atagtagata tttaataggg gtttgctgtg ggtgagcggt gcaattgcgt
     3061 gtggtgccat ggcgaatccg atgttaaaag gtgcagattt ttgctgctgc gtcggtttat
     3121 tatggtttat tgccctgaga aatccctgcc gctggataat cattaccgcc aatatccgtg
     3181 tatctgcggt gtgctttttt accaaggaat tttaaaactg ggggcttatt cacccggcgg
     3241 caaaataggt cgtcctgatc tattttgcct tcgctgcact aaacttacga aaatgcgcgg
     3301 attttcttgt tttagtgcag ctcgcggcgg ctcgcacctt tggcgcggca tccatgccgc
     3361 cattattaac ccggcactaa atgccgccgg gttaataaga agcccatgta tggggtaaaa
     3421 tgccccgctt aagcccccaa ggcggctaat gctggagtat gtgtagtata tgtcaatgtt
     3481 cacggccgct atggcgcaaa taaatccctg ggttggggat attgcaggta actgcagcaa
     3541 aattattgcc gtcagccgct cggtggctga gcagggggcg gcagactggg tgatttttcc
     3601 tgagctgacc ttgaccggtt atccgccgga ggatttgttg ctgcggccgg cccttaagcc
     3661 ccgcattgaa gcggccctgg cgcgtttatg cgccgagctg ccgcctgagt tggcggtggt
     3721 ggtgggctat cctaaagtca ccgccgacgg tgtattcaat gccgccggta ttctctatca
     3781 aggccaaatt caattggaat attgcaagca gtgcttgccc aattatcagg tcttcgacga
     3841 aaagcgttat ttcacggccg gtgccgccat gccagtttgg cagcacaagg gcagttgtgt
     3901 ggcgctctcc atttgtgaag atatttggca tccgggccct gccgcccaag caaaagccgc
     3961 tggcgcccag gtgctgatta acctcaatgc gtcgccgttt catcgcggca aacagcgcac
     4021 ccgggaggac gtagtcgccg agcgctgcgc gcaaacgggc cttgcggtgg tgtatgtaaa
     4081 ccaagtgggc ggccaggatg aattggtatt tgatggcggc tcctttgcaa tgaatagcca
     4141 aggccaattg ttagctagag ctgactattg tgttgaacag actgtgcttg tgaatcttgg
     4201 cgcccccggc agcattgcag cgccgccgtc ggatttggcc agtgtgtaca gcgcgctggt
     4261 attgggggtg cgcgactacg tgcgcaaaaa ccgcttcaaa ggagcggtgc tcggcctctc
     4321 cggcggaata gattccgccc tcacactggc tatagccgct gatgcattgg gcccggagca
     4381 ggtagatgcg gtgatgatgc cgtttcgtta cacctcggat atgagccaaa ccgatgccgc
     4441 tttgcaggcc agtttgatgg gggtgcgtta tcaaagtatt agcatcgagc ccatattcag
     4501 cgcatttgaa gcggccctca aaccggcctt tcacaatgcc gctgcagata aaaccgaaga
     4561 gaacctacag gcccgcagcc ggggtgttat tttgatggcg ctgtccaaca aaaccggcag
     4621 cctagtttta actaccggca ataaaagcga gatggcggtg ggctatgcca ccctttatgg
     4681 ggatatggct ggggggctgg atgtattaaa agatgtgcct aaaaccctgg tgttcgccct
     4741 ggcccgctac cgcaatagcc tcaatccggt gattcctgag cgggtgatta cccgcccacc
     4801 ttcggctgag ttggcaccgg ggcaaaaaga tgaagactct ctgcccccct atgaagtgct
     4861 cgaccaaata cttgccctct atatagaaga agacgccagt gcggcggata ttatcaagcg
     4921 agggtttgct gccgagacgg tgaaaaaagt tctgcgcatg gtggatttaa atgagtacaa
     4981 acgccgccag gcagccatag gtattcgcat cagccagcgc ggctttggcc gcgatcggcg
     5041 ctatcctatt accaacggtt gggcgctgga cgactaaaat cctttgtctt cacaactaac
     5101 atccattaag tgagccccta tgcacgagac ctggcgctgg tttggcccta aagaccccat
     5161 caccctagag aaaatccgcc aggcgggcgc caagggtata gtcacctcct tgcaccatat
     5221 acctactggt gaggcctggc ctctggagga tgtattggcg cgcaaacagc aaattgaggc
     5281 agcaggattg gagtggtcgg tggtggagag cattcccctg cacaatgaaa tcaagctgcg
     5341 caccggcaat ttccggcagc tcatcaaaca gtaccagcag tcaattcaag cggtggggga
     5401 ggcgggggtt aaaaccgttt gttacaactt tatgccggtg gtggattgga cccgcaccaa
     5461 tcttaattac cgcttggaca ataatgccga agccctgcgc tttgaaatga cggattttgc
     5521 cgcttatgac gtgtatatgc tgcagcgcca gggcgccgct ggcagctaca gcccggaagt
     5581 tttgagccgt gccaaatccc gttttgacgc catgagcgcc caggagcgcg cccttttaga
     5641 gcgcaatatc atcgccggtt tgcccggcgg agagggctct tacaatcgcg acggcgtgcg
     5701 cgcggccatt gaagaattta tcgccctggg cgaggggcaa tttcgcgcta atttaatcgc
     5761 cttcctgcag gaaattattc cggtggctga ggaatacggg gtgaatatgt gtgtgcaccc
     5821 ggacgatccg cctttttcat tattcggcct accccgtgtg gtttccaccg ctgcggatgt
     5881 gcgggccatt tttaccgcag tgcccagccg ccacaatggt ttaaccctct gcgcaggctc
     5941 ctttggtgcg cggggcgata acgacctagt ggccatcgcc cgagaatttg gcccagtggt
     6001 acattttgtc cacctgcgca atgtgaaacg ggaggaggac ggctcctttt ttgaagccga
     6061 gcacttagat ggcgacaacg atatggtggg tttaattagc gccctattag atgaacaagc
     6121 ccgtcgcaag gcgatggggg acgccaattg gcaaattcct atgcgcccgg atcacggcca
     6181 tttattggcc gatgaaatcg gccagccggg ggtaaaccca ggttattcct atgtggggcg
     6241 tttaaagggg ttggcggaat tgcgcggtgt tatagtcgct ctagaggccg tgcgcaaaac
     6301 gccgggtgtt tatgcgcctc aggtttttgg cccgcctagc aattagatgt atcggcgcgt
     6361 tttgccatta tgcacctgca gtatttttat ttttgcgggc atgtgtaagt agaaaattgg
     6421 cgggccagtt taaccgctgg ttgtaatact aggctgccag agcgccgccg cctgtgtatt
     6481 tcaaccagcg gttagaggtg cttattgaga atcattttaa tctgacgatt taaattcaaa
     6541 ggtacgagag taaaagtaaa attgaattta agctggctgg gcgcgctaaa attaatttca
     6601 tacgaggtaa aagtgaaaat gaacttaagt cgttggctcg attttggcct taaaaaagtc
     6661 ggtaaaaaaa cggcggccag tatttttcgg ttgatttagg acaaaaatgt atatggatac
     6721 ggatgatcaa ttagtgctgc gtctcagaat cactaaccat ttgttgtcag atatttgctg
     6781 taaaacgata tggcatataa tgcagacggt tcaattataa taatttacac atcctatgaa
     6841 agttaaaagc gccaaaccta aaaacttcaa tgcccaggtg gtcgagtaca tcggctcgcg
     6901 tattttgcac ggccagtaca acgagggaga aaaactcccg gttgagagcg agctgtcgcg
     6961 ggaattgggt gtgagccgcc caattctgcg ggaggcgaca aagattttat cctccaaagg
     7021 gttgttaagg gccaaaccca aggtgggtac cattgtgacc gagcgcaagc actggaatat
     7081 gctcgatagc gatgtgctcg gttggttgat tgaatcaaag ccccccgttg agtttttgga
     7141 catgttgttt gatgcccgct ccgccatcga gccgcgcgcg gcggaattgg ccgcattaaa
     7201 ggccactgag gccgatttag aagatattcg cctcgcctat gaagatatgg cgcgggtgga
     7261 atccatctac gagtcattcg agccggatat tcgttttcac caagctatct tggccgccac
     7321 ccataatgaa gttgttatgt atatagggca cacattacat aacgcgctgg ctatttcatt
     7381 cagtctcacc acctgggata aagagctgta tatggtggcg gtggcaaaac acaaagccat
     7441 ttacgacgca attgccgaac gcaatccaca aaaagccggt gatgccgtgc gccacttgtt
     7501 aatggcatcc agagaggact ttaatttacg ccaagctgaa gaggggcccg tgccggctct
     7561 gtcggaattg gatggattgt tctctgaagt tgaataggtg ggatctagcc acttctttta
     7621 ccctcagccg agggcgggta cgcggggtgt tttaggttaa actaatggta aatgctctta
     7681 ttggctcgtg tgccaaactc ggtgcgaatt gtctgccggt ggtggccccc gcgggcctat
     7741 gtgcaaagct cccattgatt tcgagtccac ttcatgaatt taacgaatag gtacccgagc
     7801 cttgcgggca ctttggtatt aattaccggt ggcgccacgg gcattggcgc aggtatggtg
     7861 gaggcgtttt gcctgcagcg tgcgcgggta gtttttctgg atattttgca acctgaagcc
     7921 gaacaattgg ttaataaggt tgccgcttta gatggcgcta ctgcgccgga attcttttac
     7981 tgtgatctag ccgatatccc cgccttgcag gcagttattg cctcaattga acagcgcttt
     8041 ggccctattg gtgtattaat taataacgcc gcccgcgaca cccgtcacga ctatagcacc
     8101 gtgtctgcag cctattggga tgaaatgctc agcgtgaatt tgcggcatgt gtattttgct
     8161 attcaagctg tcagtgccgg gatgaaatca ctgggaggcg gttcgattat taattttggc
     8221 tcagtcagtt ggtaccgctg ccaggctaat ttaacctgct atgcgactag taaagcggcc
     8281 ctagagggca tgacccgcag tttcgcccgc gatctaggtg ttgatggtat tcgcgtcaat
     8341 acattaatcc ccggttgggt gatgaccgag cggcaattgc gcgattgggt aaatgccgaa
     8401 acactcagtg aaatacaaaa agcccaatgc ctaaagccta atgtgcaagt ggaagacgtg
     8461 tgcgccttgg cattattttt agcctccaat gacagcaaaa tgtgcacagc acaaaatttt
     8521 attgtggatg gtggttggat ttaatggtct ggtgacattt taagcctgtg ggctgcaggg
     8581 cggtgtttca acaggttgtt attaacccgg cgtcttttta tgccgggtta ataactgcgg
     8641 caagccgccg cgagctccgc taaaacaaga aaatccacgc attttcgcca gttttagcgg
     8701 agcgagggcc aaatagatca ggacaatcta tttggccgcc ggatcaataa gtggacagat
     8761 attatctgtt gattgtaact gttcggcccc tcgttattgt agctggatat aattcccaca
     8821 ctgagtgaaa acgattgggc gcgcgcttta gcgctgcaag ccctcaatac tccctccgca
     8881 gtgatgaata caggttattt cagacacgct gtgaacacat cggtattggc tcgtcaaaaa
     8941 ggtccctctt tttgacggtc tgaaataacc tgtattcacc actgccgcag cttgaatcta
     9001 ttatcctaaa aactgcagtt gaactggtag ctagggtaac gagacgctcg atatatgcag
     9061 ttcttgtaaa aattcggtga acacagtatt caacactgcg gtgagtcgaa tttttaccaa
     9121 aaaccctgca gattcattcc tcaagcagcc ccagtagagt ttaactgcgc tttttcggat
     9181 tatgtcgcat tgtaagccac ctcaatttgc cgcttgcgcg aagagagcac atggagagag
     9241 taatgaggtg tgctgaatag ttgcgtttaa gttttatcgc ttgccatggc tttgtcttat
     9301 aaattaattg cgtagcaatt ttttcttacg aatttcccgc ctgctttttt attgttgctc
     9361 ccttggttat agtgcagcgt ttttacaggt tttaacggct agtgtgaatt tcacgctata
     9421 ctcaaatttc cgctctgtgc cgtgttttgc aggttgcaat gcgctgcctg ctgcatgtgt
     9481 caactggctg tacatgctaa aaccactctc tgtaacggcc ttagcttaaa gtcaaataat
     9541 aaatatcttt tattcatccg gcataaaatg ccgccgggtt aataattcag tactttttat
     9601 cggagtctac atatggtttt gcgatctcaa ttcagtgtgt tgtgctgcgc ggtattggca
     9661 gttgcctgtg cacaaacatc ggtaaaaccc cagggggctc tgcatccggg ggggtatttt
     9721 gcggcgccgg gtgtgaatgt gtttgccttt aatagcccgc cgggcggatt attttttgat
     9781 gctaaaacca gcggtgtgga aattattcat catggtgagc gcacggccac caatggcgat
     9841 gtgcgtatgt ttgccacccc cgagcagtgg gatggcatgg gccaattggt gagccgaaaa
     9901 gttgatgagc aggcaaaaac cattactacc caattagctt atccagagtt gggttttaat
     9961 tattccgtag tggtggcggc ttacggccag ggttataaag tgagtgtgta tttagatcag
    10021 ccgctgccac aatccttggt aaataaagcg ggctttaata tggaatttct gccgtcggtt
    10081 tattgggata aggcctatct tatggatacc aaagcgggcc aatttccccg cgctgcctcc
    10141 aatgctaatc caatgctgat gagtgccgac ggcaaaacag aaccctctcc actggcggtg
    10201 ggtaaacatt tggtattggc cccggaggat gcccagcaac gtgtggctat tcatgaagcg
    10261 ataggagata acacattaat gttattcgac ggtcgccgca aagcacaaaa cggctggtat
    10321 gtggtgcgct cgatgattcc ccagggcaaa accggcaagg tgatcgaatg ggatattgat
    10381 attaaatcta cacccaactg gattcgcgag ccggttatag gtttttcgca agtgggctat
    10441 cacccggcgc aaaccaagcg ggcgataatt gaattagacg aacatgataa agccacgcca
    10501 tccataacct tgctgcgtat taataccgat ggcagtaaaa caccggtgca aaaagcagtg
    10561 gccaccccct gggggaacta tttgcgctat caatacgccg tatttgattt tacccaaatt
    10621 aaagagccag gtttgtatca gttgcaattg ggcgcgcaga caacaccggc atttcccatc
    10681 ggcaatgagg tattggccaa tagttggcag cccacattag acttttttat gccggtgcag
    10741 atggaccata tgcgtataaa agaagcctac cgcacctggc atgggctttc ccatatggat
    10801 gacgctctgc aggcgccgcc caattacaac cattttgatt tgtatgcaca caatgctcag
    10861 ctggatacac aatacaagcc gtttgaacat attccggggc tcaatattgg cggctggtat
    10921 gatgcgggtg attttgatat tcgcactcaa tcccagtacg aaaccgtggt gggtttagtg
    10981 catgcatttg aagaattttc gattccgcgc gataacacca ccatcagcca agctaaacgc
    11041 tatgtgcaaa ttaatcgccc agatggagtg ccggatattc tgcagcaaat tgaacacggc
    11101 accctggcct taattgccca gcatcgcgct ttgggccacg ctattcccgg tattgtagaa
    11161 gggcgtttat accagtaccc gcatttgggc gatggtgcga gtaaaacgga taataaaatt
    11221 tacaataaag cattagaccc tcacgccgct agccgcaaag tgggcgaaaa ttacgagccc
    11281 tcaacggtag atccgacacc ttttgaggct ctaatgcctg aaaatggttt ggaaagtggc
    11341 cgttttgatg atcgttgggc gtttaccagc aatacctcgc cattaaatta tggctccgct
    11401 gcagccttgg ctgccgctag ccgcgtgtta aaaaccatta acccagccct tgcgcaggaa
    11461 tgtttaacca cggcccttaa agtgtggcag accgaggcaa aacgcagccc taatacctac
    11521 cacgtaggca acaccaccgg cggtaaattg gaggatgaac aattaagtgc tgcggttgaa
    11581 ttattgttga ccacccgcga tagccaatat gccaccgcta ttcgcacatt attgccatcg
    11641 gtgcgggagc gtttttatgg cggcaatgtg gaattattgg ttttggcggc gccgtttatg
    11701 gatgccgaat ttaatcagac cttgcgcagc ctggcggaaa cctatgtaac acgggccaaa
    11761 caaatgacca gcgaaaaccc ctatggagtg cctattacac gcggcagttg ggcgggtaat
    11821 ggcgtggtga tgggatttgg tatcgccaat ttctggctgc acaaaaaatt ccctgattta
    11881 gtggatgccg actgggtata ccggcctatg agttatattg tgggcacaca cccggattcc
    11941 aattattcat ttgtatccgg tgtgggggcg cagtcgcaaa ccaaggctta cggcaataat
    12001 cgggcggatt tttcttttat tcccggtggc gtggtgccag gtgtgttggt attagcgcct
    12061 gattatccag aaaacaaaag tgattggccg tttttttggg ggcaaaatga atatgtaatc
    12121 cccatggcca gccactatat attcttaagc catgcggcgg cggcattggc ggcggcacag
    12181 gagaaagcaa ccccctaaat aggagccagg gtttttagct gaagtcatct aacatttgct
    12241 gcccgcacga aagaggagca taaggtatgt tga
//
LOCUS       DAOQGS010000044        12237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2404_length_12237_cov_5.193482, whole genome shotgun sequence.
ACCESSION   DAOQGS010000044 DAOQGS010000000
VERSION     DAOQGS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_12237_cov_5.193482"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1355)
                     /locus_tag="PKZ52_02810"
     CDS             complement(180..1355)
                     /locus_tag="PKZ52_02810"
                     /inference="COORDINATES: protein motif:HMM:NF023472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HNG58728.1"
                     /translation="MRALISAFLISLITYNAFGADAPAPAAAPSPEQAAQEPAPAAAP
                     PAPPPPPLPALSVRREQQQGNLLKQWYGEQTVSLNTPDGDFVAIWQDDTSGKPLGALL
                     MLHDASQMPDWPAQLQPLRQYLSQHGWATLSISLPEPDPRVLPPRPEPPKLPPPAETA
                     KEGDAAKAEANKEEKKPAEDKPQEKTAEKAEPGETKEVFNDTTGKIGDGSLPPAQPPK
                     QEVLPSPPAEPIAQARISVALALLKEKKQFNNALLGVGLGAARAAYFALQHQGDPAQQ
                     APDGAPLTPPFQALLLINPRMQIEAVAGFNLTQALTNPKLPVLDVSYSAHPATPNSAA
                     LRKAAARKNQLAAFEQHVILEPEGSDGGEEQLNRIVRGFLMKYAKGKQIKGGEEKEP"
     gene            1447..2139
                     /gene="rpe"
                     /locus_tag="PKZ52_02815"
     CDS             1447..2139
                     /gene="rpe"
                     /locus_tag="PKZ52_02815"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNG58729.1"
                     /translation="MQVTASPDPKPYWIAPSILSADLARLGEDVARVLQAGGDLVHFD
                     VMDNHYVPNLSFGPMILKALRNYGIDAPMDVHLMVEPVDALIESFAAAGANYISFHPE
                     ASRHVDRSIELIRSQGAKAGLVLNPATPLSLIEPVLHKLDMLLLMSVNPGFGGQKFIP
                     YVLEKIAAARALIDAKGLNTRLEVDGGITAQNIQALARAGADTFVAGSAIFGSADYFT
                     AINAMRTELAGV"
     gene            2370..4022
                     /locus_tag="PKZ52_02820"
     CDS             2370..4022
                     /locus_tag="PKZ52_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HNG58730.1"
                     /translation="MHSSFLSSRTKISACAHASVSSNSVIARMFKLAALFCLIALAGC
                     DGGAIVFDAGSDGRAEVDENTSGVVWTAKATVAGTANRAKLVYELKGVDAAAFSINST
                     NGAISFKQPADFEMPVDADKNNEYLFSIEAKADTKSAVQQVALRVKNVTQPVVELIKP
                     QPFENVGTGEPVEVETQVKFYDAESNTPVADGNITLNSMPMEAAGEDAKIWKSKIAVA
                     EGGVELNIAASASTVGAINIKEKLLNKRNAANVSFPWAVQGDWMMVAGGGLDILTSFD
                     LTSGELVEPGTGLYYPLGQFDRPSVTCHPFLLVCYGIVYGSNSADLIRINYAPAAQLQ
                     ISIGQLSSPLQGNLVGLGVDAEHQRMVFVSEMLGKLNISARPLDEYGNLLGENDLFLF
                     SLPSDTFKNGHKQFAVHGRSNAFVFTEQRSLNGVLQSFIRGFSEAGVQRFEVQLGANI
                     SNLVVHESAGLIYFAENNLGSGAQLKAIDIATGVVQNMIDEARGVPHGAFTGLALDQV
                     NDRLYVADSISDSIYQVDLKNKSMGELPYRFIPYAKPPGIED"
     gene            complement(4179..4832)
                     /locus_tag="PKZ52_02825"
     CDS             complement(4179..4832)
                     /locus_tag="PKZ52_02825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNG58731.1"
                     /translation="MTLALFDLDNTLLGGDSDHAWGMFLVAKGLVDANEYSAANDRFY
                     AQYQAGTLDINAYLAFALAPLTRFSSEELGALHQEFMAGFIEPLILPKALALLDQHRS
                     LGHRLVIITATNRFITGPIATRLGVQDLLATEPEMINGRYTGNISGVPCFQGGKVVRL
                     QQWLKEQGEDLAGSYFYSDSANDLPLLEAVDHPVAVDPDARLREWAEGKGHLVMSLR"
     gene            5117..5602
                     /locus_tag="PKZ52_02830"
     CDS             5117..5602
                     /locus_tag="PKZ52_02830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNG58732.1"
                     /translation="MIDADGFRPNVGIMLSNSEGQLLWARRVGGQDAWQFPQGGIKQH
                     EDSETALFRELNEEVGLQASDVKILACTKGWLRYHLPARFVRQQEPVCIGQKQKWYLL
                     QLLSDDSRLNVNACNPPEFDAWRWVNYWYPLNQVVAFKREVYRRALKELAPFVAPAHT
                     P"
     gene            5613..7892
                     /gene="ptsP"
                     /locus_tag="PKZ52_02835"
     CDS             5613..7892
                     /gene="ptsP"
                     /locus_tag="PKZ52_02835"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNG58733.1"
                     /translation="MLTALRSIVQEVNAARDLASVLNIIVARVKAVMNTEVCSVYLRD
                     GSGAYVLMATEGLNPDSVGRVRLALNEGLVGLVAAREEPINLEHAENHPKFQYFPETG
                     EERYSAFLGVPIIHHRTVMGVLVIQQQPRRKFDEGEEAFLVTMSAQLAGVIAHAEATG
                     AITIGMENSGGEVRFQGVAGAPGVAIGQAVVISPLADLKAVPLQRIDNIPEELAFFNQ
                     CLAAVRKDVKHLGEELKARLNREEQVLFDAYLGMLDDASLGGEVRSRISQGLSAQYAW
                     SDVILEHVKTFSSMHDPYLQERASDVHDLGSRVLAYLQQSNEKIQVYPDNTILVGEEL
                     TASALGEVPFGKLAGIVSVRGSSNSHVAILARSMGIPTVMGAVDLPFTKLDRRELIVD
                     GYHGCVFSDPSAGLKERYQDIAKDDQELYRLLESLKELPCETLDRHRVALWVNTGLMA
                     DAILSLERGAEGVGLYRTEVPFMLRDRFPSEEEQRLAYREQLQAFAPHPVTMRTLDIG
                     GDKALPYFPIAEDNPFLGWRGIRVTLDHPEIFLAQVRAMLKASEDLNNLRILLPMISS
                     IPELEIAQMLIYRAYDELVEEGYRISMPPIGVMIEVPSAVYLAKEMAARADFLSVGSN
                     DLTQYLLAVDRNNPRVADLYHSLHPAVIRALQQVVEDSHSQGVPVGICGELAGDPSAA
                     LLLMAMGFDSLSMSATNLLRVKSVIRGVKLADAQALLAEVKQLPDTESINRLIQRAYN
                     EAGLTWLTKPDMPGQVKTI"
     gene            8096..8908
                     /gene="lgt"
                     /locus_tag="PKZ52_02840"
     CDS             8096..8908
                     /gene="lgt"
                     /locus_tag="PKZ52_02840"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNG58734.1"
                     /translation="MLTYPKIDPVAFSLGPLKVHWYGLMYLVGFTVSWYLAKKRSRRA
                     DLVVREHQVDDLIFYGAMGVVLGARIGYVLFYKPGEFFADPSMIIRVWEGGMSFHGGL
                     LGVIIAMLLYCRKIGQKFLDVMDFVAPFCPVGLFFGRIGNFIGAELYGRAADVPWAMV
                     FPTDSEHLPRHPSQLYEAGLEGLLLFIILFVWYSAKPRPRGAVCSLFVLLYGCFRFFV
                     EFFRQPDAHINFEWFGWMSRGQELCLPMIFIGGGVFIWAHLNARKTQLQLGK"
     gene            8912..9745
                     /locus_tag="PKZ52_02845"
     CDS             8912..9745
                     /locus_tag="PKZ52_02845"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNG58735.1"
                     /translation="MQQYLDLMRHVYEHGITRSDRTGTGTKSVFGYQMRFDLAAGFPL
                     VTTKKVHLKSIIHELLWFLQGATNIKYLQDNGVKIWNEWATAEGELGPVYGAQWRSWA
                     GPNGEVIDQISQVIEQIKHKPHSRRLIVSAWNPALLPDESRAPHENALEGKMALPPCH
                     TLFQFYVLDGKLSCQLYQRSGDIFLGVPFNIASYALLTMMVAQVTGLALGEFIHTLGD
                     AHLYLNHLEQVQTQLSRAPLPLPQMVINPAVTDIFKFTFDDFRLENYQSHPAISAPIS
                     V"
     gene            9784..10299
                     /locus_tag="PKZ52_02850"
     CDS             9784..10299
                     /locus_tag="PKZ52_02850"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNG58736.1"
                     /translation="MPKLAIICAMSLNRVIGRNNQLPWHLPADLQFFKRTTLGKPIIM
                     GRKTYESIGRPLPGRTNIVITRQQHLAYEGVHLATSLPMAIDKARQAAAMAEQKEFFV
                     IGGADLYAQALPLAQALYITQVQAHIDGDAYFPEWDKSAWQLIRSEHHAVDDANQYAM
                     EFQHFEPAIGI"
     gene            10550..11950
                     /gene="ppnN"
                     /locus_tag="PKZ52_02855"
     CDS             10550..11950
                     /gene="ppnN"
                     /locus_tag="PKZ52_02855"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HNG58737.1"
                     /translation="MHNPHSANSHKKPLKIKAARVTPEHSLDVLSQLEVMHLAKSSHT
                     NMYETFRRCALAVLNTGEETDDTSKILEAYDDFSIEVIQQERGIKLEIHNAPANAFVD
                     GEMISGIKEHLFSVLRDLIYISNELQHSRLLDMENSEGVTNSVFHILRNAGLFNPQNE
                     VNLIVCWGGHSINEVEYQYTKKVGYALGLRGLDICTGCGPGAMKGPMKGATIAHAKQR
                     INGRYIGITEPGIIAAESPNPIVNKLIIMPDIEKRLEAFVRAGHGIVVFPGGAGTAEE
                     ILYILGILLNPANADMPFPLVFTGPKESADYFKQIHEFIGLTLGPQAQRLYTIIIDDP
                     DQVATYMQARMRDVQAFRRDTQDAYYFNWVIAVDEELQRPFKPTHANMTQLNLHKNQE
                     PHRLAAQLRKAFSGIVDGNVKDGGIKAVKEFGPYEIHGEKAIMQPLEALLRSFVAQKR
                     MKIPTQEYIPCYKIVS"
BASE COUNT         2737 a         3128 c         3339 g         3033 t
ORIGIN      
        1 cctacacgga tgtattcacg gcgtgtctga aataacctgt attcatcact gcgggaatta
       61 ctgagggctc gcagcacccg cgcatttgcc cggtgatttc tgtgcggcac gggaataagg
      121 tccagctaca ctaaaacggt gctgagtccc ttcgttttcg gcaattcaga tgcggcaacc
      181 taaggttctt tttcctcccc gccttttatc tgtttaccct tggcgtattt cattaaaaat
      241 ccgcgcacta tgcggttgag ttgttcctcg ccgccgtcgg aaccctccgg ctctaaaatc
      301 acatgctgct caaatgccgc taattggttt ttgcgcgccg ccgctttgcg cagggcggcg
      361 ctgttggggg tggcggggtg tgcgctgtag ctcacatcca gcaccggcag tttgggattg
      421 gtgagggcct gggtgaggtt aaagcccgcc accgcctcaa tctgcatgcg cgggttaatc
      481 agcaacagcg cctgaaacgg cggcgttaag ggcgcgccat cgggggcctg ctgggccgga
      541 tcgccctggt gttgcagggc aaaataggcg gcgcgggctg cccccaggcc cacccccaag
      601 agggcgttat taaactgttt tttttctttc agcagagcca gggcaacact gatgcgggcc
      661 tgggcgattg gctcggctgg tgggctgggt agcacctcct gcttcggcgg ctgggcgggg
      721 ggcaggctgc cgtcgccaat tttgccggtg gtatcgttga acacctcttt ggtttcacca
      781 ggctctgctt tctcggcggt tttttcctgc ggtttatcct cagccggttt tttttcctct
      841 ttattagctt ccgctttagc ggcatctccc tctttggcag tctcagccgg gggcggcaat
      901 ttcggcggct ccgggcgcgg tggcagtacc cgtgggtccg gctccggcag gctaatagaa
      961 agtgtggccc agccgtgttg gctgaggtac tggcgcaggg gttgtaattg cgcgggccag
     1021 tccggcatct ggctggcatc gtgcagcatg agcagggcgc ccaggggttt accgctggtg
     1081 tcatcctgcc agatcgccac aaaatcccca tcaggcgtgt taaggcttac cgtctgctcg
     1141 ccgtaccatt gcttgagtaa attaccctgc tgttgctccc ggcgcacact taaggcgggt
     1201 aggggcggtg gcggcggagc tgggggcgcg gctgccgggg caggctcctg ggcggcctgt
     1261 tctggactgg gggcggcggc tggggcaggc gcatcagcgc caaaggcgtt gtatgtgatt
     1321 aggcttatga gaaaagcgct gatgagtgct cgcattctgg ctacaatccc ctttccagcg
     1381 cccgctcctg ggctttttag aatttgctaa acgatactgc catcagcttg gcataaggcg
     1441 gcgaagatgc aagtaacagc ctcacccgat cccaaaccct actggattgc cccctcgatt
     1501 ttgtcggcgg acctcgcccg gctaggggag gatgtagcgc gagtgctgca ggcggggggc
     1561 gacctggtgc attttgatgt gatggataac cactatgtgc ccaacctcag cttcggcccg
     1621 atgattctca aggccctgcg caactacggt atagacgcgc ctatggacgt gcatttaatg
     1681 gtggagccag tggatgcctt aattgaatcc ttcgccgccg caggcgctaa ttacattagt
     1741 tttcacccag aggcgagccg ccatgtggat cgctccatcg aattaattcg cagccagggt
     1801 gcaaaagcag gcctagtatt aaaccccgcg actcccttaa gtcttattga gcctgtattg
     1861 cataaactcg atatgctgtt attaatgagt gtaaacccag gctttggcgg acaaaaattt
     1921 attccctacg tattagaaaa aatcgccgcc gcccgcgcat taattgacgc aaaaggctta
     1981 aatactcgct tagaagtaga cggcggtatc accgcgcaga atattcaagc cctcgcccgc
     2041 gcaggggcgg atacctttgt ggcgggttcg gcaatttttg gttcggcgga ttattttacc
     2101 gctattaatg ctatgcgcac tgagttggct ggggtgtaat aaatacgcgt aagcgcccgc
     2161 ttttacctct tataaaattc attttttggc ccgttttctt tgttacccag tcctcaaaat
     2221 aacgcgaata cgtaaattta cgtatttccc ctgaaaactc ccaagcgtaa agtccagcct
     2281 attgcttcgt ataagcgcgg ggataggcgg gatcattcct gccctcgtca atctggaaca
     2341 ggttttttgc aaccgccttg ggagtttaca tgcacagtag ttttttatct tctcgcacca
     2401 agatctcggc ttgcgctcat gcgtcagtca gctctaactc tgttattgct cgtatgttta
     2461 agctcgcagc cctattttgt ttaatagctt tagcagggtg cgatggcggt gcgattgtgt
     2521 ttgacgctgg cagcgatggc cgcgcggaag ttgacgaaaa tacttcgggc gtagtttgga
     2581 cggcaaaagc tactgtggcg ggcacggcta atagggctaa attggtttat gagcttaaag
     2641 gcgttgacgc cgcagcgttt tcgattaatt ccaccaacgg cgccattagt tttaaacagc
     2701 ctgccgattt tgaaatgccg gtggatgcgg ataaaaataa tgaatatcta ttttccatag
     2761 aggccaaggc ggataccaaa tcggcagtgc agcaggttgc attgcgagtt aaaaatgtca
     2821 cgcaaccagt ggtagagctt ataaagccgc agccctttga aaatgtgggc actggcgaac
     2881 cggtggaggt ggaaacccag gttaaatttt acgatgcaga atccaatacc cccgttgctg
     2941 acggcaatat caccctgaat tcaatgccca tggaagcagc tggtgaagat gcaaaaattt
     3001 ggaagagtaa aatagctgtg gcagaagggg gtgttgagtt aaatatagcg gcttcggcaa
     3061 gcaccgtcgg cgcaattaat attaaggaaa agcttttgaa taaacgcaat gccgcaaacg
     3121 tgagttttcc gtgggcagtg cagggggatt ggatgatggt ggccggtgga gggcttgata
     3181 ttttaacgag ttttgatctt acatccggcg aattggttga gccgggcacc ggcctttatt
     3241 atccgttggg gcagttcgat aggccgagtg taacctgtca tccatttctg ctcgtatgtt
     3301 atggcatcgt ttacgggtca aacagtgccg atttaattcg cattaactat gctccggccg
     3361 cgcagctcca aatctcgata ggtcaactat cctcccctct gcagggaaac ctagtcggct
     3421 tgggtgtaga tgctgaacat cagcgtatgg tttttgtgtc tgagatgctt ggaaaattaa
     3481 atatctcggc acgtccgctt gatgagtatg gcaacctttt gggcgagaat gatttgtttt
     3541 tgtttagctt gccaagtgat acttttaaaa acggtcataa gcagttcgcg gtgcatggcc
     3601 gctcgaatgc ttttgttttc acggagcagc gaagcctcaa tggtgttttg caatctttta
     3661 ttcgagggtt tagtgaggct ggtgttcagc gctttgaagt gcaattgggc gcgaatattt
     3721 cgaacttggt tgtgcatgaa tctgcagggc taatatattt tgccgaaaat aatctagggt
     3781 ccggcgcaca attaaaggcg attgatattg caactggagt tgtgcagaac atgattgatg
     3841 aggcgcgtgg agttccccat ggcgccttca ctgggttggc attggatcag gtaaatgatc
     3901 gactctacgt agcagattcc ataagtgatt cgatttatca agtggatcta aaaaacaaat
     3961 ccatgggcga attgccttat aggttcattc cctatgctaa acccccgggc attgaagact
     4021 aataagatgc tattttaacg ctcacgaaca ctctgtcgat ttctatagcc tttgttatag
     4081 aaatcgacga gcctggatgg ctgcaataaa ttcagttgta acgcgccaat taactttatt
     4141 cttgctcgga aaatcctaaa actttgagtg cgacgctctc aacgcaagct cataaccaaa
     4201 tgccccttcc cctctgccca ttcccgcaag cgcgcatctg gatccactgc caccgggtgg
     4261 tccaccgcct ccaacagcgg taaatcatta gccgagtcgc tgtaaaaata actgcccgct
     4321 aaatcctccc cctgctcctt aagccattgt tgcaggcgca ctaccttgcc tccttgaaaa
     4381 caaggcacgc cgctgatgtt gccggtgtag cggccattga tcatttccgg ttccgtggcc
     4441 aataaatcct gcacacccaa acgcgtggca atgggcccgg taataaagcg gttggtggcg
     4501 gtgatgatga ctaggcggtg gccaaggctg cggtgttgat cgagcagcgc cagggctttg
     4561 ggcaaaatca acggctcaat aaaacctgcc ataaattcct gatgcagagc accaagctcc
     4621 tcgctgctaa aacgggtaag gggggcgagg gcgaaagcta aataggcgtt gatgtctagg
     4681 gtgcctgcct gatactgcgc gtaaaagcgg tcgttggcgg cactgtattc atttgcgtct
     4741 accaggccct tggccactaa aaacatgccc caggcgtggt cgctgtcgcc gcctaataag
     4801 gtgttgtcta aatcaaataa cgctagggtc acaaaaactc caaaaaataa ggggatatgg
     4861 ctgggggttg gcgcggcttt ggctatgctt gccttagggc ccataggggt gagctaagct
     4921 gctgtgcagc ataaagattt tcttccaaca ggcggaaatt caggtacatt accaggctaa
     4981 accagcagtt cctaattgtg gcggcggcag cgctaaaagt gaatgcgtaa accctttagg
     5041 gcgcaggcac caccagcgtt tggggcgatc tgtgccgcca ctttggcaaa ctttatacgt
     5101 aaattgagag gccccagtga tagacgccga tgggttccgt cccaatgttg gcattatgct
     5161 tagtaactcc gaagggcaat tgctctgggc tcgtagggtc ggggggcagg atgcctggca
     5221 gtttccgcag ggcggtatta aacagcacga agacagtgaa actgctttat ttcgcgagtt
     5281 aaatgaagaa gttgggctgc aggcgagcga cgtgaaaatt ctcgcctgta ccaaaggctg
     5341 gctgcgttac cacctgccgg cgcggtttgt gcgccagcag gagccggtgt gtatcggcca
     5401 gaaacaaaag tggtatttgt tgcagttgct cagtgatgac agccgcttaa acgtcaatgc
     5461 ctgtaacccg ccggaatttg acgcctggcg ctgggtgaat tactggtatc cgctgaacca
     5521 ggtggtggcg tttaaacggg aggtctaccg ccgcgcactg aaagaattgg cgccctttgt
     5581 ggcgcccgca cacacaccct aagggcaccc ttatgctcac cgcgctacgc agtattgttc
     5641 aggaggttaa cgccgcccgg gatcttgcct cggtgctgaa tattattgtg gcgcgcgtaa
     5701 aggcggtgat gaacactgag gtgtgctcgg tgtatctgcg cgatggcagc ggcgcctatg
     5761 tgctgatggc caccgagggc ttaaacccgg attcagtggg ccgggtgcgc cttgctctca
     5821 atgagggctt ggtgggtttg gtggcggcgc gggaagagcc catcaacctt gagcacgcag
     5881 agaatcaccc caaattccag tacttcccgg aaaccggtga agagcgctac agcgcctttt
     5941 taggcgtgcc gattatccat caccgcacgg tgatgggggt gttggtcatc cagcagcagc
     6001 cccgccgcaa gttcgatgag ggcgaagaag cctttttagt caccatgtcc gcccagctgg
     6061 ctggtgtgat cgcccatgcc gaggccactg gcgctattac catcggtatg gaaaacagcg
     6121 gtggcgaggt gcgctttcag ggcgttgcgg gtgcccctgg tgtagccata ggccaggcgg
     6181 tggtgatctc cccgctggcg gatttaaaag cggtgcccct gcagcgcatc gacaatatcc
     6241 cggaagaatt agcctttttt aatcagtgtt tggccgccgt gcgcaaggat gtcaagcatt
     6301 taggtgaaga gttaaaagcg cgcttaaacc gcgaggaaca ggttctattt gatgcctatt
     6361 tgggcatgct cgatgacgcc tcgttgggcg gtgaagtgcg ctcgcgcatc agccagggct
     6421 taagcgctca atacgcctgg agcgacgtga tcctagagca cgtcaaaacc ttctccagca
     6481 tgcacgaccc ttacctgcaa gagcgcgcct ccgatgtgca cgacttaggc agccgggtgc
     6541 tggcctatct gcagcagtcc aatgaaaaaa tccaggtcta cccggataac actattttgg
     6601 tgggggagga gctaaccgcc tcggccttgg gtgaggtgcc gtttggcaaa ctcgccggta
     6661 tcgtcagtgt gcgcggctcc agcaactccc acgtggctat tttggcccgc tccatgggca
     6721 tccccacggt tatgggggcg gtggatttac cgtttaccaa gctggatcgc cgcgagctga
     6781 tcgtcgatgg ttaccacggc tgcgtcttta gcgacccttc ggcggggtta aaagagcgct
     6841 accaagacat agccaaggac gaccaggagc tgtaccggct gctggaatcc ttaaaagaat
     6901 taccctgcga aaccctggat cgccacagag tagccctgtg ggtaaacaca ggcttgatgg
     6961 ccgacgctat tttgtccttg gagcgcggtg ccgaaggcgt gggcctgtac cgtaccgaag
     7021 tgccctttat gctgcgggat cgctttccct cggaggagga gcagcgcctg gcctaccgcg
     7081 agcagttgca agcctttgcg ccccacccgg tcaccatgcg taccctggat ataggcgggg
     7141 ataaggcctt gccctatttc cccatcgccg aggacaaccc gtttttgggc tggcgcggca
     7201 tccgggtgac gctggatcac ccagagatat tcttagccca ggtgcgggcc atgctgaagg
     7261 cgtcggagga tttaaataat ctgcgcattc tgctgcccat gatctcctcc atcccggagc
     7321 tggaaatcgc tcaaatgctg atttaccgcg cctatgacga actggtggaa gagggctatc
     7381 gcatcagtat gccgcccatc ggcgtgatga tcgaagtgcc ctcggcggta tatttggcga
     7441 aagaaatggc ggctcgggcg gattttctct ctgtcggcag caacgactta acccagtatc
     7501 tgctggcggt ggatcgcaac aacccccgcg tggcggatct ctaccactcc ctgcatccgg
     7561 cagtgattcg cgccctgcag caggtggtag aggactccca cagccagggc gtgccggtgg
     7621 ggatctgcgg tgagttggcc ggggacccat ccgcggcact tctgttaatg gccatggggt
     7681 ttgatagcct gtccatgagc gccactaacc tgctgcgggt caagtccgtc attcgcggcg
     7741 ttaagctagc ggacgcgcag gccctgttag ccgaggtgaa acagctgccg gataccgagt
     7801 ccatcaaccg gttgatccag cgggcgtaca acgaggccgg gctcacttgg cttaccaagc
     7861 cggatatgcc ggggcaggtg aaaactatct aatatccgcc agcgctatac ccttaggttt
     7921 aaggcaccct tttgttttgt tggtggttgc cgtcccattg tttaccgctg aactaaatgc
     7981 cgctgagtga ttcgattaag ctctgctttc gatccagcat ggctgctagc gattcgagca
     8041 tgaatataat taagctgggt tttaccccaa agaacttgcc attttaggaa ccataatgct
     8101 gacctacccc aaaattgatc ctgtagcctt ttccctcggc cccttaaaag tccactggta
     8161 tgggcttatg tatttagtgg gttttaccgt ctcctggtat ttggccaaaa aacgcagccg
     8221 ccgtgcagat ttagtggtgc gcgagcacca ggtggacgat ttaatttttt acggcgccat
     8281 gggcgtggtg ttgggtgcgc gcattggcta tgttttattt tataaacccg gcgaattttt
     8341 tgccgatccc tcgatgatta ttcgcgtctg ggagggcggt atgtctttcc acggtgggtt
     8401 attgggcgtg attattgcga tgctgttgta ctgccgcaaa atcggccaaa aatttttaga
     8461 tgtgatggac tttgtcgcac ctttttgccc ggtgggcctg ttttttggcc gtatcggtaa
     8521 ttttatcggt gctgaattat acggccgcgc cgctgatgtg ccctgggcca tggtatttcc
     8581 caccgattct gaacacctgc cgcgccaccc ctcgcaactg tatgaagccg gattagaagg
     8641 cctgctgtta tttattattt tatttgtatg gtattccgcc aagcctcgcc cccgtggcgc
     8701 tgtgtgctcc ctatttgtgt tgttatacgg ctgcttccgc ttttttgtgg agtttttccg
     8761 ccagcccgat gcgcatatta attttgaatg gttcggctgg atgtcgcgcg gccaggaatt
     8821 gtgtttgccg atgattttta ttggcggcgg cgtatttatt tgggcgcacc tcaacgcccg
     8881 caaaacgcag ctacagttgg gtaaataaac catgcagcag taccttgatt taatgcgcca
     8941 tgtgtacgaa cacggcatta cccgcagcga ccgcaccggc accggcacca aaagtgtatt
     9001 tggctatcaa atgcgctttg atttagccgc aggcttcccg ctggttacca ctaaaaaagt
     9061 gcatttaaaa tccatcatcc atgaattgct gtggtttttg cagggcgcta ccaatattaa
     9121 atacctgcag gataatggcg taaaaatttg gaatgaatgg gccacggcgg agggtgaatt
     9181 aggcccggtg tacggcgccc agtggcgctc ctgggcgggg cctaatggcg aggtgattga
     9241 tcaaatcagc caggtgattg agcagattaa acacaagccc cattcgcggc gcttaattgt
     9301 ttcggcctgg aacccagcgc ttttgcccga tgaatcccgc gccccccatg aaaatgcgct
     9361 ggagggcaaa atggctttgc cgccctgcca cacactgttt cagttttatg tgttggacgg
     9421 caaattatcc tgccagctct accagcgcag cggcgatata tttttagggg tgccatttaa
     9481 tatcgcctcc tacgccctgt taaccatgat ggtggcccag gtgacgggct tagccttggg
     9541 ggaatttatt cataccctcg gcgatgccca cctatattta aaccatttag aacaggtgca
     9601 aacgcagtta agccgcgcgc cactgccctt gccgcaaatg gtaattaacc ctgcggttac
     9661 ggatattttt aagttcacct tcgatgattt tcgcctagaa aattaccaat ctcaccctgc
     9721 tattagtgcg ccgatttccg tttgatggtg tatcggcgcc ttttttatta ctgtgtttaa
     9781 tctatgccta aacttgccat tatttgcgct atgtcgctca accgagtgat tggccgcaac
     9841 aatcaattgc cctggcattt acccgccgat ttacagtttt ttaaacgcac caccctgggt
     9901 aagccgataa ttatgggccg caaaacctat gaatccatcg gccgcccttt gccgggccgc
     9961 accaatatag ttattacccg ccagcaacac ttagcctatg agggggtgca cctggccacc
    10021 agtttgccca tggccattga taaggcccgc caggcagcag ccatggccga gcaaaaagaa
    10081 ttttttgtaa ttgggggggc ggacctgtac gcgcaggcct tgccccttgc ccaggcgctc
    10141 tatatcaccc aggtgcaggc ccacatagac ggcgatgcct atttcccaga gtgggataaa
    10201 agtgcctggc aattaatccg cagcgagcac cacgcagttg atgatgccaa tcagtatgca
    10261 atggaatttc agcattttga gccagccatt ggtatttgat ccgcatgttg cactcgcccg
    10321 ttttgtcgcg ccgctacagg gcaaccatcg cggtgggctg tgggccacac ggcggcgtaa
    10381 aagcggctag acttaagcta acccttgtaa attaaccctt tgaattgacg cgtgttttaa
    10441 aaatcaaagc gattgcggcg cagcataagg cgccctctgc ctgcacttgc acggatggtc
    10501 accagcaagt ctgcggcttc aaataaaaca caaaaattag gatgttgcta tgcacaaccc
    10561 acactctgcg aacagccaca aaaaacccct taaaattaag gccgcccgcg taacccctga
    10621 acacagtttg gatgttttat cgcaattgga agtgatgcac ctggctaaat ccagccacac
    10681 caatatgtat gaaaccttcc gccgctgcgc tttggcggta ttaaataccg gcgaggaaac
    10741 cgacgatacc agtaaaatac ttgaagccta cgatgatttt tccatcgagg tgattcaaca
    10801 ggagcgcggt attaaattag aaatccacaa tgcgcccgcc aatgcctttg tggatgggga
    10861 aatgatcagc ggtatcaaag aacatttatt ctccgtgctg cgggatttaa tttatatcag
    10921 caacgaattg cagcacagcc gtctgctgga tatggaaaac agtgagggtg ttaccaactc
    10981 ggtatttcat attttgcgca acgcaggcct gttcaacccg caaaatgaag tgaatttaat
    11041 agtctgctgg ggcggccact ctattaatga agtggaatac caatacacca aaaaagtcgg
    11101 ttacgcttta ggcctgcgcg ggttggatat ttgtaccggc tgcggccccg gcgctatgaa
    11161 aggccccatg aaaggtgcca ccattgccca cgccaaacag cgtattaacg gccgttatat
    11221 aggtattacc gagccgggta ttatcgctgc ggaatcgccc aacccgattg tcaataaact
    11281 cattattatg cccgatatcg aaaaacgcct agaggcgttt gtacgcgcag gccacggtat
    11341 tgtggtattt ccagggggcg caggcacggc ggaagaaatt ctgtatattt tgggcatttt
    11401 attaaacccc gccaatgccg atatgccgtt ccctctggtg tttacggggc cgaaggaatc
    11461 ggcggattat tttaaacaaa tccacgaatt tatcgggcta accctcgggc cccaggccca
    11521 gcgtttatac accattatta tcgacgaccc ggatcaagtt gctacctaca tgcaagcgcg
    11581 catgcgcgat gtgcaggcgt ttcgccgcga tacccaggat gcttattatt ttaattgggt
    11641 gatagccgtg gatgaagaat tacagcgccc ctttaaaccc acccacgcca atatgacgca
    11701 attaaacctg cataaaaacc aagagcccca ccgcttggcg gcgcaattgc gcaaggcatt
    11761 ttcggggatt gtggatggca atgtaaaaga cggcggcatt aaggcggtga aagaatttgg
    11821 cccctatgaa attcacggcg aaaaagccat tatgcagccg ctagaggcct tgttgcgctc
    11881 attcgtagcg caaaaacgca tgaaaatccc cacccaggaa tatattccct gctacaagat
    11941 tgtcagttaa gcaactaagg cctaagggtt tgttgaaata cctaatggcc tattgcaaga
    12001 cgtcctggtt aagttttccg tatagcaagg attttttacc agtgcagcag cgggttgtta
    12061 aaaaacacag ccgcgttgtt attaaggcaa aagcccgcta cagcgtaagt tggaagagcg
    12121 cagcgacttc cgacatctat ccgccagtat aaactctggg cacggcaagg cctcggcagg
    12181 atgtcggaag tcgctgcgct cttccaacct tgtatctctc agcaaagcgg attaact
//
LOCUS       DAOQGS010000045        12147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2428_length_12147_cov_4.723619, whole genome shotgun sequence.
ACCESSION   DAOQGS010000045 DAOQGS010000000
VERSION     DAOQGS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_12147_cov_4.723619"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKZ52_02860"
     CDS             <1..451
                     /locus_tag="PKZ52_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNG58738.1"
                     /translation="ESVRADWTQLAKEFQTTLYEKIFTEQDYSDYIQTTVKQVLAGEH
                     NKDLIFRKRLRRPLSSYQKTTPPHIQAARKYQTWTGEILKQGDWIDYVMTVNGAEPLG
                     VDEDFALLKSPIDYEFYINKQLKPIADSILYFLGGKMQAGPAQLALF"
     gene            complement(614..>956)
                     /locus_tag="PKZ52_02865"
     CDS             complement(614..>956)
                     /locus_tag="PKZ52_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNG58739.1"
                     /translation="PAIEPRKAVMREAAQRGFATATDLADYLVRKGIAFRDAHEIVGK
                     AVGYGVQTQKDLAEMTLAELQVFSSEIQDDVFAVLTLEGSVAARDHIGGTAPNQVRFA
                     ITQAKAELASR"
     assembly_gap    957..970
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<971..2021)
                     /gene="argH"
                     /locus_tag="PKZ52_02870"
     CDS             complement(<971..2021)
                     /gene="argH"
                     /locus_tag="PKZ52_02870"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNG58740.1"
                     /translation="MSDSSQSTSTLWGGRFSEATDAFVQRFTASVNFDKRMYRQDIQG
                     SIAHATMLASVGVLSEAERDAIIQGLSDIQKEIEAGTFNWSVELEDVHMNIESVLTAR
                     IGITGKKLHTGRSRNDQVATDIRLYLRDEIDVIAREITRLQQGILQLAEANTDTIMPG
                     FTHLQTAQPVTFGHHLMAWYEMLSRDYGRLMGCRKRLNQSPLGAAALAGTTYPINRNY
                     SAELLGFNHPTRNSLDSVSDRDFAIEFSSFAAILMTHLSRASEELILWTSAQFNFINL
                     PDRFCTGSSIMPQKKNPDVPELVRGKSGRVNGNLIALLTLMKSQPLAYNKDNQEDKEP
                     IFDTVDTVRDCLRAFA"
     gene            2159..3208
                     /locus_tag="PKZ52_02875"
     CDS             2159..3208
                     /locus_tag="PKZ52_02875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNG58741.1"
                     /translation="MSEVTRSPLHATVQGDFLPNLCTLEAVFRLVILGELLALALVLA
                     DLGVKEFDLAVFGMLSLMVQWVVLVSAAGLCRLAPWFRKLKPWQSTLLAYAWILTAST
                     AIGFAALYIQVESLKLLWPSLATQILITMVIAGVALRYFYVQQQLQEQIKAELRARIQ
                     ALQSRIRPHFLFNSMNSIASLIAIDPERAEAMVEDLSDLFRASLSEPGLVPLAKELDI
                     CRRFVRIEQTRIGERLKMVWEIDPSALEVKIPSLLLQPLIENAIYHGIGPLPEGGEVK
                     VSARLEQDQCWLEVSNPLPDPSTLNTKGNGLALENIHRRLSAYFAGKTGWQTQIDQGR
                     YVLCVQFPAKITESA"
     gene            3282..4022
                     /locus_tag="PKZ52_02880"
     CDS             3282..4022
                     /locus_tag="PKZ52_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNG58742.1"
                     /translation="MHVLVVDDELLARQRAMRMIESLDDYEVVGEAGNGQAALAAIAD
                     LDPDIVLLDIRMPGEDGLEAARKIAEMDEPPAIIFCTAYDEYALEAFSTLAVGYLLKP
                     LQKDALKNALEKARRLNKVQRQELPEAESAPREAARKHIAARTRRGVELILIDDISSF
                     VADQKYVTVRHKGAETLIDDTLKDLEREFAGRFIRIHRNCLVAIHLIEKLERIPGGQY
                     ELHLKDTPYRPVVSRRHVAGVKELLSSL"
     gene            4746..5168
                     /gene="hpaR"
                     /locus_tag="PKZ52_02885"
     CDS             4746..5168
                     /gene="hpaR"
                     /locus_tag="PKZ52_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HNG58743.1"
                     /translation="MMNTTVEELPILLQKAREYAVNTMRNTLATHDLTEQQWRVIRST
                     YESGELNAQELARKSAILGPSLSRILNRLEEDGILNRKVSAADQRELNISLTAKGKRL
                     HNKVLPTLNTQYDEIGKQIGNAKLEQLAGLLNYVISLQ"
     gene            5390..6319
                     /locus_tag="PKZ52_02890"
     CDS             5390..6319
                     /locus_tag="PKZ52_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNG58744.1"
                     /translation="MAGTSLLVLIDDIAAVLDDVAVMTKVAAKKTAGVLGDDLALNAE
                     QVAGIRAERELPVVWAVAKGSFINKAILVPAALLISAFANFLIGPLLMLGGAYLCFEG
                     VEKLAHKWLHPVKDDHITKLEAALDDPQVDMVAFEAQKIKGAVRTDFVLSAEIIVITL
                     GTVALSPFSQQLVVVTLIALLMTVGVYGFVALIVKLDDIGVHLMQKSPHRSSLSYRFG
                     ELLLTAAPKLMKFLSIAGTVAMFLVGGGILSHNLAPIHHAVEALQSALVNLPLAGLWQ
                     LLATSAADCITGVLAGVIALLGVKGLGKIRGRN"
     gene            complement(6322..6789)
                     /locus_tag="PKZ52_02895"
     CDS             complement(6322..6789)
                     /locus_tag="PKZ52_02895"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNG58745.1"
                     /translation="MLTPAHQSSPPRSAPLWRRFAALGYDSLILMALSMGYGALITSV
                     KVALGTPGHDYQPMLKSPLYFLGWLSVLIGFFLYSWCRSGQTIGMRAWRIQVIPQDPT
                     QKALSVKQALARCLFGAISMLGLGAGYWYSWFNREGNTWHDAMTHSKVVLIPK"
     gene            6996..7169
                     /locus_tag="PKZ52_02900"
     CDS             6996..7169
                     /locus_tag="PKZ52_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58746.1"
                     /translation="MGLDVLDHATEVSGQGYEEVNVDKDTPKVDTDSRRRLEDKLEEM
                     RLRKELREYDFDI"
     gene            complement(7613..7957)
                     /locus_tag="PKZ52_02905"
     CDS             complement(7613..7957)
                     /locus_tag="PKZ52_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNG58747.1"
                     /translation="MRYSTFFASALLASSLLAATASQAAPVLDSLRVIPPVQELNLPE
                     PPAAPVLDLLVGNEFSPIQLALPDLTLDRGPITRPSKPRFQVPEPNGLFLLSLGVFSM
                     GVLRLRTKNQTL"
     gene            8404..8748
                     /gene="fliE"
                     /locus_tag="PKZ52_02910"
     CDS             8404..8748
                     /gene="fliE"
                     /locus_tag="PKZ52_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNG58748.1"
                     /translation="MVDRVDINRLLLEMRTLKSQTQAFGGVSPVEGKMAPQGTTGVAF
                     GDVMSQAVNKVNEVQQASSSLSESYIRGDRSVDISDVMIASQKASVAFSAMTQVRNKL
                     VDAYKDIMNMPV"
     gene            8762..10459
                     /gene="fliF"
                     /locus_tag="PKZ52_02915"
     CDS             8762..10459
                     /gene="fliF"
                     /locus_tag="PKZ52_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNG58749.1"
                     /translation="MAASETAAGAEPRSGNYLIQGFNNLNLLRQAGLMVGLAASVAIG
                     FAVVLWTQGEDYRPLYGSLEHLDSKEVTQILESNEIRFKVDENSGALLVGAKDVHKAR
                     LKLAEAGIPNNNNSVGFELLDKEQPLGTSQFMEGARYRRSLEGELARTIASVTSVKSA
                     RVHLAIPKASVFVRDNHVPSASVFVELFPGRGIDPNQVRGIANLVASSVPELKPENVT
                     VVDQKGALLSVGVEDAKLTVASQHLDYTRKVENDMVLRVRRLLSPILGDDNFKTEVSA
                     DLDFSEVEQAQESFNPDLPALRSEQTSEEIKKGPGADGGIPGALTNQPPASAAAPEVA
                     KTGADGKTQTTSTEQSNSRTQATRNFELDRTVSFTKHEKGKLKRISLAVVVNDKVVTD
                     PATGQKAKMRWSDAELERLAALVRDAVGYSAARGDSVNVMNEAFIDADGTETFAQPWW
                     QTDWFKSIAKQGAGIFIILVLIVGLLRPVLKSLASSGLQSRAEEEAKEMEALRAAGVD
                     GFDSLSDETVTLTGGDSLALPSPEQSYEQQLNAVKALVAEDPGRVAQVIKRWINENE"
     gene            10452..11495
                     /gene="fliG"
                     /locus_tag="PKZ52_02920"
     CDS             10452..11495
                     /gene="fliG"
                     /locus_tag="PKZ52_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNG58750.1"
                     /translation="MSKNAPAPADGAEGRPPSKLTRTEQAAILLMSVGEVTAAEILKH
                     MGPKEVQRLGTAMTQLKDVQQYEVEVVLSNFMAEVRTQTGLGLGSDSYISNMLVTALG
                     EDKANGLIDRILLGGNTTGLDTLKWMEARSVADIIRNEHPQIQAIVLAYLEADQSAEI
                     LTYFPEKVRLDVMMRVAALDSVQPSALQELNNILEKQFSGAAGSQTKQMGGYKTAAEI
                     MNNLDSSIEGELMNAIKEIDEDLGTQIADLMFVFDNLKDVDDRGIQALLREVSSEVLI
                     LALKGADEDLREKIFKNMSKRAAELMRDDLETKGPVKISEVEGAQKEILTIARRMADA
                     GEINLGGSGETMV"
     gene            11565..>12147
                     /locus_tag="PKZ52_02925"
     CDS             11565..>12147
                     /locus_tag="PKZ52_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNG58751.1"
                     /translation="MSKLPGRIPFEDVEKFRNWAVPKVSGQVLASAEREAKKAQQANL
                     PKQEHETVEEIAAEQVSLGPMTAEQLAQITDEASREGFNQGYEEGLEKGLATGERQGQ
                     EQGKTQALAEWSGRLKAQVEQLAATCEALLDPLGQQHQRLTEEMLNLSVLLAEQLLQR
                     ELSQNPAAYWGWLQEALAQLPAGRKDASLFLPAE"
BASE COUNT         2808 a         3055 c         3472 g         2798 t
ORIGIN      
        1 agaatccgtg cgggcagatt ggacgcaact ggccaaagaa tttcaaacca ccctgtacga
       61 gaaaatattc accgagcaag actacagcga ttatattcaa actaccgtta aacaggtgtt
      121 ggcaggcgag cacaataaag accttatctt ccgcaaacgc ctgcgccgcc ccttaagcag
      181 ctatcaaaaa accacaccgc cccatattca ggcggcgcgc aaatatcaaa cctggacggg
      241 tgaaatatta aagcagggag attggattga ttatgtgatg acagtgaatg gcgctgagcc
      301 attaggtgtt gatgaggact ttgctttgtt aaaaagcccc attgattacg agttttacat
      361 caataaacaa ttaaagccta ttgccgatag cattttgtat tttttgggtg gcaagatgca
      421 ggctgggcct gcgcagttgg cgttatttta atacgctaca gtgttttgct gtcggatgtc
      481 ggtaaagggc cgctgtggaa attcattcgc agtgcatggc gcacttaagt gcgccctact
      541 aagcctctct aaatgtgaat tttaaacgcc tacaacacca ggggaaaaaa tccaccatcc
      601 agcagactgt aaatcaacgg ctcgccaatt ccgctttagc ctgagtaatg gcaaaacgca
      661 cctgattggg cgctgtaccg ccaatatgat cccgcgcggc aactgagcct tccagcgtta
      721 atactgcaaa tacatcatcc tgaatttcgc tggaaaacac ctgcaattcc gcaagcgtca
      781 tttcggccaa gtctttttgc gtttgtacac cgtaacccac tgctttaccg acaatttcat
      841 gggcgtcgcg aaaggcgata cctttgcgca ctaaataatc ggctaggtcc gtggcggtag
      901 cgaagccgcg ttgggccgct tcgcgcataa cggctttgcg cggttcaatg gcggggnnnn
      961 nnnnnnnnnn cggcaaatgc ccgcaggcaa tcgcgcactg tatccaccgt gtcaaaaatg
     1021 ggctctttat cttcttggtt atctttgttg taggccaagg gctggctttt catgagggtg
     1081 agtaatgcga ttaaattgcc gttcacccgg ccacttttgc cgcgcactaa ttctggcacg
     1141 tctgggtttt ttttctgcgg catgatggag gagccggtac agaagcggtc gggcaaattt
     1201 ataaaattaa attgcgccga ggtccagaga attaattctt cactggcgcg ggataggtgg
     1261 gtcattaaaa tcgccgcaaa gctactaaat tcgatagcaa aatcccggtc gcttacggaa
     1321 tccaaggaat tgcgggtggg gtggttaaaa cccagtaatt cagcgctgta attgcggtta
     1381 atggggtagg tggtgccagc taatgccgcc gcgcccagtg ggctttggtt gaggcgtttg
     1441 cggcagccca ttaaacggcc gtaatcccgg ctgagcattt cataccaggc cattaaatgg
     1501 tggccgaagg tgacgggctg ggcagtttgt aaatgggtaa aacccggcat gatggtatcg
     1561 gtattggctt cggccaattg cagtatgccc tgttgcaatc gggtgatttc ccgtgcaatc
     1621 acatcaattt catcgcgtaa atacaggcga atatcggtgg ctacctggtc gttgcgggag
     1681 cggccggtgt gcagtttttt accggtaatg ccgatgcggg cagtaagtac cgattcaata
     1741 ttcatgtgca catcttctaa ctctaccgac cagttaaaag tcccagcttc aatttctttt
     1801 tggatatcac tcaggccctg aataatggca tcgcgctcag cctcgctaag tacacccacc
     1861 gaggccaaca tagtggcgtg ggcgatggag ccttggatgt cttggcgata catgcgttta
     1921 tcgaaattaa ctgaggcagt gaagcgttgc acaaaggcgt cggtggcttc gctaaaacgg
     1981 ccaccccaaa gggtgctagt ggattgtgat gagtcgctca tagggctcct gaggtcggcg
     2041 gtgggcgcaa aaagcggcta ttatccgcta ctttgccgct aaaccccagt gcgcagcgca
     2101 gtggctcggc ggcataagcg ctttatgtgg attgctgaac ctgttgagga gtaaacccgt
     2161 gtctgaagtt acccgctcgc cgctgcacgc cactgtgcaa ggggattttt tgcccaacct
     2221 gtgcaccctg gaggcggtgt ttcgcctggt gattctcggc gagttactgg ctttggcgct
     2281 ggtgctcgct gacttgggcg ttaaggagtt tgatttagcc gtgttcggca tgttaagcct
     2341 tatggtgcaa tgggtggtgt tggtgagcgc ggcggggttg tgccgcttgg cgccttggtt
     2401 tcgcaaatta aaaccctggc aatcaacgct gttggcctac gcctggattt taaccgcctc
     2461 caccgccatc ggctttgccg cgttatatat tcaggtggaa agcttaaaac tgctgtggcc
     2521 gagtttggct acccaaatct tgattaccat ggtaattgcc ggagtggccc tgcgttattt
     2581 ctacgtgcag cagcaattgc aggagcaaat caaagccgag ctgcgggcgc gtattcaggc
     2641 tctgcagtcc cgcatccgcc cgcattttct ctttaatagt atgaattcca tcgccagcct
     2701 gatcgctata gacccggagc gggccgaagc catggtggag gatctgtcgg atctgttccg
     2761 agccagtttg agtgaaccgg gcctggtgcc tctggctaaa gagttggata tctgccgccg
     2821 ctttgtgcgc atcgagcaaa cccgtatcgg cgagcgctta aaaatggtct gggagataga
     2881 cccctctgct ctggaggtaa aaattcccag cctattactg caacccttga ttgaaaacgc
     2941 catctaccac ggcatcggcc cgctgccgga aggtggcgag gtgaaggtga gcgcccgcct
     3001 ggagcaggac caatgttggc tggaagtcag taacccactg ccagacccaa gcaccctgaa
     3061 caccaagggc aatggcttgg ccctggaaaa tattcaccgc cgtttaagtg cctatttcgc
     3121 aggcaaaacc ggctggcaga cacaaattga ccaagggcgt tatgttttgt gcgtgcagtt
     3181 tccggccaaa atcacggaaa gcgcctaaac tcttcagatg tgtttggcga ccttaagtag
     3241 ccttaacctg tgtcctaaga atagagtgag tgatccaacc tatgcatgtg ttggttgtcg
     3301 acgatgagct gttagcccgc caacgggcga tgcgtatgat tgaatccctc gatgattacg
     3361 aggtagtagg ggaggctggc aacggccagg ccgccctggc ggctattgcg gatttagacc
     3421 cggatatagt gttgttggac atacgtatgc ccggtgaaga cggcctggaa gccgcgcgta
     3481 aaatcgctga aatggatgaa ccgccagcca tcattttttg caccgcctac gacgaatacg
     3541 ccctggaggc ctttagcacc ttggccgtgg gttatctgct caaacccttg caaaaagatg
     3601 ccctgaagaa cgcattggaa aaggcccgca ggctgaataa ggtacagcgc caggaattgc
     3661 ctgaagccga aagcgccccc agagaggccg cccgcaaaca catcgccgct agaacccggc
     3721 gcggcgtgga gctgatttta atcgacgata tcagcagttt tgtcgccgac cagaaatacg
     3781 tcacggtacg ccacaagggt gctgagacgc tcatcgacga taccctcaaa gacctggagc
     3841 gggagtttgc aggccgcttc attcgcattc atcgcaattg tttagtggcg attcatctaa
     3901 ttgaaaagct tgagcgtatt ccaggggggc agtacgagct gcacctgaaa gatactccat
     3961 accgcccagt tgttagccgc aggcatgtgg ctggcgttaa agagttgctc tcaagccttt
     4021 aacctaaaaa ccgcagttgt actctactac ggccgcctaa tgcttgaatc tgcacgcttt
     4081 tttgtcaaaa tttgactcac cgtagtggtg actactgctt tcgccaaatt ttgacaaaaa
     4141 agcgcccata ttcagcgcct taggcgccct cgctacgagt tcaactgcgg tttttaggtt
     4201 taaggccatg ctgttgattc ctgccggggc caattgagaa tgctttggat cccttgccgt
     4261 tcttcttaac ctcttttgca taaatagttg tagttgaact gactgtatca tcggatggtt
     4321 gaagtggccc cggcgagagg gttttcttaa ggagacctta agggcaggcg gtcagtatgc
     4381 cgccccgcca ataccgggcc attgttcgtg tcttgacgtg aatgtgtgca ctgacgaggc
     4441 ggcgagccag agcgccaggc gcttgacggc taaggcgggt ccgatccgct gttaaccatt
     4501 ggccccaagt ggttaataag tttattagtt cacaaaataa ataacgcagc acaaatgttc
     4561 taaaaaaaat gcatttatgg gtggcgcaca aatcattctt tggttaatat gtgaacagta
     4621 gtcagatggt agcgatgaag tgctcctgta gcccggttct ccaccgaggt tccatggtct
     4681 tttaaggccc tgaccatgga ttttgctgga aacccctatt tatcaatccc gaagatgtaa
     4741 ccgctatgat gaatactact gttgaagaac ttcccatcct gctccaaaaa gcccgcgagt
     4801 acgcggttaa caccatgcgc aataccctcg ccacccatga tttaaccgag caacaatggc
     4861 gagtcattcg ctccacctat gaaagcggtg agttaaatgc acaggagctt gcccgcaaga
     4921 gcgcaatttt gggccccagc ctgtcgcgga ttctcaaccg gttagaggaa gacggcatac
     4981 tcaatcgcaa agtgtccgcc gctgatcagc gcgagttaaa tatctcgcta acggccaagg
     5041 gcaagcgctt gcacaataag gtattaccca ccttaaacac ccagtacgac gaaattggca
     5101 agcagattgg caacgccaag ttggaacagc tggcaggtct gctcaattac gttatttcac
     5161 tgcagtgata ctattgattc tctcagctca gaaagcccgg ttcgccgggc ttttttattg
     5221 gcggatctga tccttttctt gttccgcgct cctttgtgtt tatctccttt gaatagacgt
     5281 ttgttggtga ggcgagatca ttcgtggtga tttggctttt ggaaccctgt gattaggcgg
     5341 cttgcgcaat cttaagtaca ctgacgcgct taattttctt gggggttcta tggccggtac
     5401 aagcctattg gtgttaattg atgatatagc ggcggtgttg gacgatgtgg cggtgatgac
     5461 caaagttgcc gctaaaaaaa ccgccggcgt gctgggggac gacctggcgt taaatgcgga
     5521 gcaggtggca ggcatacggg ctgagcggga gctgccggtg gtttgggccg ttgccaaagg
     5581 ctcgtttatt aacaaggcta ttctggtacc ggccgcccta ctcattagcg cctttgccaa
     5641 tttcttgatc ggcccattgc tgatgctggg cggggcctac ctgtgttttg aaggggtgga
     5701 gaagcttgcc cacaagtggt tgcaccctgt taaggatgat cacatcacta aacttgaagc
     5761 cgctttagat gacccacagg tggatatggt ggcgtttgaa gcgcagaaaa ttaagggtgc
     5821 ggtgcgcacg gactttgttc tttcagcgga aattattgtc attaccctcg gcacagtggc
     5881 tttaagcccc ttttcccaac agctggttgt ggtgacccta attgcactgc tgatgaccgt
     5941 cggggtatac ggttttgtag ccttgatcgt caaacttgac gacataggtg tgcacctaat
     6001 gcagaaaagc ccgcaccgat ccagcctgtc ttatcgcttt ggtgaattgc tgttaacagc
     6061 cgcgcccaag cttatgaagt tcctctccat cgccggcacc gtggcgatgt ttttagtcgg
     6121 tggcggcatc ctcagccaca acctcgcgcc cattcaccac gcggtggagg ccctgcaaag
     6181 cgccctggta aacctgccct tggcaggcct ttggcagctg ctggcgacca gcgcggctga
     6241 ctgtatcacc ggtgtgttag cgggtgttat cgcgctcttg ggggttaaag gcttagggaa
     6301 aatacgcggc cgcaactagg tttactttgg gatgagcact accttcgagt gcgtcatggc
     6361 gtcgtgccag gtgttgccct cgcggttaaa ccagctatac cagtaacccg cccccaagcc
     6421 gagcatactg atcgcgccga acaggcagcg ggccaaggct tgtttaacgc tgagggcttt
     6481 ttgggtgggg tcttggggaa taacttggat gcgccaggcg cgcataccaa tggtttgccc
     6541 ggagcggcac cagctgtaga gaaaaaaccc gattaacacg cttagccagc cgagaaaata
     6601 tagcgggctt tttagcatgg gttgatagtc gtgccccggc gtgcccaggg ctactttgac
     6661 actggtaatt aacgcgccat atcccatact taaagccatc aggatgaggc tgtcgtaacc
     6721 taaagcagca aagcgccgcc agagtggggc gctgcgaggt ggcgaagatt ggtgtgcagg
     6781 tgtgagcata aatgtgtcga aaaggcgcca gtttaacggg ttggtaaagc cctgacatcc
     6841 ttgcggttta cttctgtaaa taagccaatt taagagttaa aaattatttg tatttttttc
     6901 tcgtttaggc atactcgcgc gaactgaggt gcacctgagc ctgtgggagc aagtggtgcg
     6961 acttttttat taatatcgct acaggagctt atgttatggg tcttgacgtt cttgatcatg
     7021 ccacagaggt tagtggtcag ggttatgaag aagtgaatgt tgataaagat acgcccaagg
     7081 tggatacgga ttctcgccga cggcttgagg ataagttaga agagatgcgg ctgcgtaaag
     7141 aattgcgcga atacgatttc gatatttaac caatcccctc tccaattaaa ggctgtgttt
     7201 cacagccttt tttgtttctg ccgccgttat tttcctcagc tcttgtttat tcctgttagc
     7261 ccggctaggc catcgctcaa aggcatctac gcatttgccc agtgagtgct cgttaatgcc
     7321 cggcaaactc caccaggccg gggcaatgta gttaagttaa ggattccctg tacagcactg
     7381 ggagcgccct ttgtgggtgc ccgatcccgc gtaaattctt atctgcagct gaggcaaaaa
     7441 ctgggcaacc acgagggttg cccctacgga attgcctgaa ccttatgaca gtggttagca
     7501 aggaggtcaa ttgaagggaa aacgcgcccg cacgcgcagg ggcaaggtat taacttaagt
     7561 ggttgcaggc tgggggggag ttgatggcgt ccttgccatc gcaatggagg gattaaaggg
     7621 tttgattttt ggtgcgtagg cgcaatacac ccatactaaa tacgcccaag ctcaacagaa
     7681 acagaccgtt gggttctggg acttgaaagc gcggtttgct ggggcgagtg atcgggccgc
     7741 gatcaagggt gaggtcgggg agggcgagct gtatgggtga aaactcgttg ccgaccaaca
     7801 gatccagtac aggggctgcc ggtggttccg gcaggttaag ctcctgcacg ggtgggataa
     7861 cacgcaggct gtcgaggacg ggagcggctt ggctggcggt tgcagccagc aggctgctgg
     7921 caagcagggc ggaggcaaaa aaggttgagt agcgcatggg tgacaaatcc ttaagatggc
     7981 ttgggtggcg tcatcttagc agtatccagt ccatagtaaa aaagctattt aaaaacaatt
     8041 aattaagtgt tgtttttggt cctgccttga tgagcgtgtt agatttctcg acacttaacc
     8101 tgcgcataaa aagtcagcac ctgaggcccg ccgagtaggc catatgtgtc agcctcaggc
     8161 gcctggttgc ctaacctgct agatgttgcg ccggttcaaa gccccctccg attagcccgc
     8221 taaaaaccct tctccctcat cttgtttacc cctcaacccc atcccacggc ttttgcgccg
     8281 cagcgaaaac ttcgacaccg aacctaagac taaggtgtgg atttttggcc taaaaaatgc
     8341 tcctacggta tgagttcaat aaattaggcc aaaacgacag aattttgtcg ccccggagtg
     8401 cttatggtcg accgagtaga catcaaccgc ttgctgttag aaatgcgcac cctaaaaagc
     8461 cagacccagg ccttcggtgg ggtttcgcct gtggagggga aaatggcccc acagggcacc
     8521 acaggtgttg cctttggtga tgtgatgagc caggcggtga acaaggtgaa tgaagtgcag
     8581 caggcgtcga gcagtttgtc ggaatcctat attcgcggcg atcgcagtgt ggatatttcg
     8641 gatgtgatga tcgcctccca gaaggcctcc gtggctttta gcgctatgac tcaggtgcgc
     8701 aataagctgg tggacgccta caaagacatt atgaatatgc cggtttaagg cggggtgagt
     8761 tgtggctgct agcgaaactg ccgccggagc tgaaccgcgc tccggcaatt atttaatcca
     8821 gggctttaat aaccttaatc tgctgcgcca ggccggcctg atggtggggc tggccgcctc
     8881 ggtggcgatt gggtttgcgg tggtgctttg gacccagggt gaggattacc ggccgctgta
     8941 tggctctttg gaacacttgg attccaagga agtaacgcaa attctcgaat ccaacgaaat
     9001 tcgctttaag gtggatgaaa actcaggggc gctgttggtg ggcgccaaag acgttcacaa
     9061 ggcccgcttg aaattggccg aagcgggtat tcccaataac aataattccg tcggctttga
     9121 gctgctggac aaggagcagc cccttggcac cagccagttt atggaagggg ctcgctaccg
     9181 ccgcagtttg gaaggggagc tggcgcgcac catcgccagt gtcacctcgg tgaaatcggc
     9241 acgcgtacat ttggctattc ctaaggcttc ggttttcgtg cgcgacaacc atgtgccttc
     9301 ggcttcagta tttgtggagt tgtttcccgg ccgcggtata gaccccaacc aggtgcgcgg
     9361 tatcgccaat ttagtggctt ccagtgtgcc ggaattaaag cccgaaaacg ttacggtggt
     9421 ggatcaaaag ggcgctttgc tctcggtggg ggtggaagat gccaagctca cggtggcctc
     9481 ccagcatttg gattacaccc gcaaagttga gaatgacatg gtgctgcggg tgcgccggtt
     9541 attaagcccc attctgggcg acgataattt taaaactgag gtgagtgcgg atttggattt
     9601 cagcgaggtg gaacaggccc aggaatcctt caacccggat ctgcccgccc tgcgcagcga
     9661 acagaccagc gaggagataa aaaaaggtcc aggggcggac ggcgggattc ccggtgcgct
     9721 taccaatcag ccccccgcct cggcggcagc gccggaagtg gcgaagaccg gcgccgatgg
     9781 caaaacccaa accaccagca cggagcaatc caactcccgc acccaagcca cccgcaattt
     9841 tgagttggac cgcacggtga gtttcaccaa gcatgaaaaa ggcaagctca agcgcatcag
     9901 cctggcggtg gtggtgaacg ataaggtggt aaccgaccct gccacagggc aaaaagccaa
     9961 gatgcgctgg agcgatgcgg aactggagcg cttggcggcc ctggtgcgcg atgctgtggg
    10021 ttactcagcg gcgcgggggg atagcgtgaa tgtgatgaac gaggcattta ttgacgcgga
    10081 cggcaccgag acctttgcgc agccctggtg gcaaaccgat tggtttaaat ccatcgcaaa
    10141 acaaggcgcg ggcatattta tcatcctagt attaatcgtg gggctgctgc gccctgtgct
    10201 caaaagcctc gcttcgtccg gtttgcaatc ccgcgccgag gaggaggcca aagagatgga
    10261 ggccctcaga gctgctggcg tggatgggtt tgattccctc tctgacgaga cggtgaccct
    10321 caccggcggc gactccctgg ccctgcccag cccagagcag agctatgagc agcagcttaa
    10381 cgccgtgaaa gcgctggtgg ctgaagaccc cggccgcgtg gcccaagtga tcaaacgctg
    10441 gattaacgaa aatgagtaag aacgcacctg cacccgccga tggcgctgag ggcagacccc
    10501 ccagcaaact cacccgcacc gaacaggcgg ccattttgct gatgtcggtg ggggaggtga
    10561 cggcggcgga aatcctcaaa cacatggggc caaaggaggt gcagcgcctg ggcaccgcca
    10621 tgacccagtt aaaagatgtg cagcagtatg aggtggaagt ggtgctctcc aactttatgg
    10681 cggaggtgcg cacccaaacc ggcctgggtt tgggctcgga cagttatatc agcaatatgc
    10741 tggtcaccgc tttgggcgag gacaaggcca atggcttaat cgaccggatt ttacttggcg
    10801 gcaataccac tggcttggat actctcaaat ggatggaggc ccgctcggtg gcggacatta
    10861 tccgcaacga gcacccgcaa attcaggcca ttgtgttggc ctatttagag gccgatcaat
    10921 cggccgaaat cctcacctac ttccccgaaa aagtccgctt ggatgtgatg atgcgggtgg
    10981 cagcgctgga ttcggtgcag ccgagcgccc tgcaggagct taacaatatt ctggagaagc
    11041 agttctccgg cgcggcgggc tcacaaacca agcagatggg cggctacaaa accgctgccg
    11101 agattatgaa caacctagac agctccatcg aaggcgagct gatgaacgcc atcaaggaga
    11161 tagacgagga tttgggcacc cagatcgccg atttgatgtt tgtcttcgac aacctcaaag
    11221 acgtggacga ccggggcatt caggccctgt tgcgggaggt gtcatccgaa gtacttattc
    11281 tcgctctcaa gggtgccgac gaagacctgc gggagaagat cttcaaaaac atgtccaaac
    11341 gggccgccga acttatgcgg gacgatttgg aaaccaaggg cccggtcaaa atctccgagg
    11401 tagaaggcgc gcagaaggaa attctcacca ttgcgcggcg catggccgac gccggggaga
    11461 tcaacctggg tggctccggc gagactatgg tgtagccatg ctggtggggc tgtttaacgc
    11521 tgggccttgc tgcccgttct tttcctgttg gcaggtagcg ccctatgtct aaattaccgg
    11581 gccgcattcc ctttgaggat gtagagaagt ttcgcaattg ggcggtgcct aaggtgagcg
    11641 gccaggtgct ggccagcgcc gagcgggagg cgaaaaaagc ccagcaggct aacctgccca
    11701 agcaggagca cgaaaccgtg gaggagattg ccgccgagca ggtgagcctc ggccccatga
    11761 ccgccgaaca actggcgcag atcaccgacg aagccagccg ggaaggcttt aaccaaggct
    11821 atgaagaggg cctggaaaag ggcctggcca ccggcgagcg ccagggccag gagcagggta
    11881 aaactcaggc tctagccgag tggagcgggc ggctgaaagc ccaagtggag caactggcgg
    11941 ctacctgcga agccctgttg gatccattgg ggcagcaaca tcagcgcctt accgaagaaa
    12001 tgttgaattt aagtgtgctg ctggcggagc agctattgca gcgggagctg agccaaaatc
    12061 ccgccgctta ttggggctgg ctgcaagaag cccttgccca actgcccgct ggccgcaagg
    12121 atgccagcct atttttacct gccgagg
//
LOCUS       DAOQGS010000046        11991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2469_length_11991_cov_4.575905, whole genome shotgun sequence.
ACCESSION   DAOQGS010000046 DAOQGS010000000
VERSION     DAOQGS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2469_length_11991_cov_4.575905"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..368)
                     /locus_tag="PKZ52_02930"
     CDS             complement(84..368)
                     /locus_tag="PKZ52_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58752.1"
                     /translation="MLGPDSALSEDQQRQFLLAKLHQKQTFNTWLDKHNGPELTSAAL
                     TEALDTYKNNYLQDIRGVINDKQFRLIQDYEKVQFDQMGRSLRISTGLDP"
     gene            complement(411..980)
                     /locus_tag="PKZ52_02935"
     CDS             complement(411..980)
                     /locus_tag="PKZ52_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58753.1"
                     /translation="MKISLVALAACICLLSVLVFSMWRQNVQLQIHLDELSEQLSRKA
                     AEVKSAEQTWQNQLQKAAELLADAKSENANLQLQLLQLRGEEKTPVTVGTQALLSRIE
                     DNNLGDVIAHKYQYFFPGLSLSASEKDQLKRLLLDRERVLNAATSGYFSDSLDVGTKV
                     AHQQALVADIDAQIALILDDREYAEFELF"
     gene            complement(1184..1777)
                     /locus_tag="PKZ52_02940"
     CDS             complement(1184..1777)
                     /locus_tag="PKZ52_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58754.1"
                     /translation="MTYSARAVLILVLVVLVGCAQTLRTRVNSFRAPEPISTSASVAV
                     VPSESSLAQSLEFGLYKSQLERALVKQGFALAAPQQAQLHAILSYSVSRVADTGDNGV
                     RTGVMVGSNRGSFGSNVMILDGARKSGWYERKVGVVLERNNNERTRLYEINAQSEGAC
                     GVLSAVVPDMLTALFNQFPMANGAVKFVSVPVSEKCQ"
     gene            complement(1777..2268)
                     /locus_tag="PKZ52_02945"
     CDS             complement(1777..2268)
                     /locus_tag="PKZ52_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNG58755.1"
                     /translation="MINGQILVAYQNGDNVIRMLGDVRLTLCISFDNFIAQMFDARPF
                     RSVIFDLREATCIDSTTLGLMAKIAITSRKFGFKKPLVLTANPSINRLLETMGFNDIF
                     EMVSEAKLPANLETILDATDGENVDEEQVRCRVIEAHKILMQLNSSNLSQFKALVDAL
                     EKQ"
     gene            complement(2265..3458)
                     /locus_tag="PKZ52_02950"
     CDS             complement(2265..3458)
                     /locus_tag="PKZ52_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNG58756.1"
                     /translation="MISDLRTLLVIDHDESVRHTIVAMLQDSDYGVHLETSSQGGLQW
                     FIAHRPDLVLVDLTMPDIDGINLMLALREQDNAVSVIVTSAVGSARDVVEALRKGATD
                     YLIKPIQNIDFLTHAVNRALVSRDLRRENEQYKHDLERANLELRDYVRILECDQQAAR
                     QVQRNLLPATPAKVAGVTLEHKVIPSLYLSGDFVDYGGIQNRYVAFYLTDVSGHGASS
                     AFVTIWLKPLVRRLFKDCRSQEEEVNLLRDPSLLVQYVNKELLHSHFGCHLTCFVGVL
                     DTHERVLSYVTAGHLPLPILVEGGRARYLDGKGKPVGIFADNRWEVFHEQVADGFNLV
                     VFSDGILDTLPADGLLEREAYLLERMQGLEGGLGGVLARLNLTAERAGPDDIAILSLE
                     QRGRL"
     gene            3777..4082
                     /locus_tag="PKZ52_02955"
     CDS             3777..4082
                     /locus_tag="PKZ52_02955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG58757.1"
                     /translation="MELSTRNYQEKRSFIRMKLDCPAKILLESEELTGVCSNLSGGGM
                     LLVLDAPINLGTECVVSISSHFGHGPMLRAKTKVIRAMSCDGKCHAGLSIVELLDNQ"
     gene            complement(4182..4925)
                     /locus_tag="PKZ52_02960"
     CDS             complement(4182..4925)
                     /locus_tag="PKZ52_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNG58758.1"
                     /translation="MGHLLRRAGLAVAVVIWGAAGAPAALAAAGGQETNDPWEGYNRK
                     AFAFNDFFDRWLLKPVVKGYKFIAPAPVETGISNAFENLLEPASVLNDILQWKWKAAG
                     RDLGRFVVNSTAGVVGLWDVADMMGWKKPTSSEDFGQTLRTWGVPTGPYFVIPFLGPS
                     TLTEAFVATPVDFFTHPVSYTESVRAYNSLSALKIIDKRSQLLAAEELISGDRYIFIR
                     EGFLQNRAFNAADGQVIDDFGADTDLKDF"
     gene            complement(5031..6146)
                     /locus_tag="PKZ52_02965"
     CDS             complement(5031..6146)
                     /locus_tag="PKZ52_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNG58759.1"
                     /translation="MSSKLRSLIALSCLAPAGAMADAPTVYGRINLTAQQNSTETLQF
                     VSASDTVTTTKSDTTTLENNVSRIGFKGSEKFADLGLEGFYLAEYAVYADDGVDGTDP
                     NTTSIRQRNIYFGLKGSFGAAQAGYFDTPLKSVQNKVDLFNDLQGDINNYITVNDYRR
                     KNSVMYTTPKMSGFTGYIDLILSEGNSKEQDAINKEKGRDNAASLALTYEQGGFYGAI
                     AFDKNVVVVESNKNLNDESQVYRLVAQYTLGAWQLGALYDNHDATTYTAAADNKSLVG
                     KENQGGWIVSSQYTLGQWALKAQYGQSDILRGAEVERDADVLSLGADYKLSKNTRLFG
                     FYTTESADVSWQKTKSTGTVTASQGNDYFALGGELIF"
     gene            complement(6839..10729)
                     /gene="hrpA"
                     /locus_tag="PKZ52_02970"
     CDS             complement(6839..10729)
                     /gene="hrpA"
                     /locus_tag="PKZ52_02970"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNG58760.1"
                     /translation="MTQCDVFDQTACLLVDVPKLQQIARTIEQRKKAGQPFDRLQQQF
                     TALLEQSAQTLSARRAYTEVIEFPTQLPISEKREEIAALIAAHQVVVLAGETGSGKTT
                     QLPKICLTLGLGQRGLIGHTQPRRLAARAVAERIAAELNSPLGQRVGYQVRFSDLTAP
                     NSQIKLMTDGILLAEIQTDPLLLRYDALIIDEAHERSLNIDFILGYLKRLLPKRPDLK
                     IIITSATIDLQRFSRHFHGAPIIEVSGRTYPVEIRYRPSITEEDQIDAIVSAAEEILQ
                     LPKHAGDILVFLSGEREIRDTAKALRALNRPDLIILPLYARLSNAEQNRIFAQARGRK
                     IILATNVAETSITVPGIGYVIDPGFARISRYSHRSKLQRLPIEAISKASANQRAGRCG
                     RVASGICIRLYDEQDFNNRADFTDAEIMRTNLAAVILQMLSIGVGDIRKFPFVDPPDA
                     RLISDGFKLLEELNAVTPQGQLTAVGRALAKLPIDPKLARILLAANQRGCLTEMLIIT
                     TAMANQDPRERPADKQQASDEKHRRFWHADSDFLTWVALWEYIEEQRQALTASQFKKL
                     CEKEFLSWLRLREWRELHHQLRLACKEAGFKENTQPASYDAIHKALLAGLSANIGVKS
                     EEPSEQDYLGARNRKFFIFPASSQFKKRPKWILAANLLETSKVYGHTVAKIDPEWLLE
                     TAEHLLKHHYYQPHYAHQTGQVMAYDRITLYGLVIKDKHRINYAHINPQETREVFIRQ
                     ALVEGGYGRNPPHKIGEFYRHNLACIEEVQGLEAKSRRKDILVDDEEIFTFYAERLPE
                     TIANLASFEHWRKTCEREQPRLLFIPMARLKRHDAQAITEAQFPKILAVDGMELPLSY
                     HFEPGAADDGVSVHVPIDILHNLPQGPLDWLVPGLIRDKLIALIKCMPKQWRKRFVPI
                     PDAVDKILARISLGKRSLLEVLSEELQRLAGEKLPAEVWADIDIDPFYVMNIKVMDDQ
                     NRLIAQGRDLADLRLKYRANVQQSLQTAGDQVERSGITRWDFGVLEAESTLMRGKVRI
                     RAYPALIDQGASLDLKLLDNPLEAAWLSAKGLSRLALLAMPDACKYLHKNLFKKTSIL
                     LSTLGLGTPAQVVDDLLLAAAYQAYFSQHDTPRNAEAFTQLLVARSALTKTAVEYEHV
                     LEAIFTQVLALKNQMKTAKNALAVFDTVAAINEQISHWLYPGFIFATPFLQLQQMPRY
                     ISALQTRFEKAQQSPLKDKQCNQEVLIFWQKHQQLWQQKGPAVYFHNSAWQEYRWLIE
                     EFRVSLFAQQLKTRVPASAKRLTKAWEALLEMGW"
     gene            10870..>11991
                     /locus_tag="PKZ52_02975"
     CDS             10870..>11991
                     /locus_tag="PKZ52_02975"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNG58761.1"
                     /translation="MRLYIAEKPSLGRALAAVLPGPQRKQDGFIQTADGACVTWCIGH
                     LLELAEPEDYDASLKTWRLEALPFIPKQFRFKAKRQTAKQLAVVKKLIKSAKEIVHVG
                     DPDREGQLLVDEVLWEARLTAAQLSQVKRLLINDLNPAAIGRALTQVRPNSDFYALSQ
                     SALARTHADWLYGLNMTRAYSILGQSKGARGVLSVGRVQTPVLGLVVRRDEQIAAFRP
                     HDYYEVIAEVEFNGTPAARVNAKWLPSEACAPFCDTEGRVLSKPLCEHVIKRIQGKPA
                     LVDEFTTQRKNQNAPLPYSLSSLQIDAAKQLKLSAQTVLDLCQSLYENHQLITYPRSD
                     SRHLPQEHWHEAANIINTIRHNAQPLAAAAAQTDCTLRSK"
BASE COUNT         3125 a         3108 c         2801 g         2957 t
ORIGIN      
        1 tttggcaaat tcaacgacgc cagagcgagt aaggtaattt ttcagaggtt ccctaagtac
       61 ttgttagccg acttgactgt tgactagggg tctaggcctg tggaaatacg caggctgcgc
      121 cccatctgat cgaactgcac tttttcgtag tcttgtatta aacgaaattg tttgtcgtta
      181 ataacaccgc gaatatcctg caaataatta tttttataag tatccaaggc ctcagttaag
      241 gccgcgctgg tcagctctgg gccattgtgt ttgtcgagcc aagtgttaaa ggtttgcttt
      301 tgatgtaatt tagccagcaa aaattgccgc tgctggtctt cgcttaaagc gctgtctggc
      361 cctaacacgc gggctatttg ttttacctga tactgcttaa aacctgaatc ttaaaataat
      421 tcaaattcag cgtattccct atcatccaaa atcagcgcaa tctgcgcgtc tatatcggca
      481 accaacgcct gctgatgggc aaccttagta cccacatcca acgaatcaga aaaatacccg
      541 ctggttgcgg catttaatac ccgctcacgg tccagcaaca accgcttaag ttgatctttt
      601 tcactggcag aaagcgatag gccagggaaa aaatactgat atttatgggc gatgacatca
      661 cctaaattgt tatcttcgat gcggctcaat aaggcctgag tcccaactgt gacaggcgtt
      721 ttttcttcac cgcgcaattg cagtaactgc aactgcaaat tggcattttc acttttggca
      781 tcagccaata actctgcagc tttttgcaat tgattctgcc aagtttgctc agcgcttttc
      841 acctcagcag ctttgcggct caactgttcc gatagttcat caagatggat ttgcaattga
      901 acattttgcc gccacatgct gaacaccaac acacttaaca aacatataca ggccgctaaa
      961 gccactaaac ttattttcat agccaactca agtgcgagcg aatcaaaaca acctcacagg
     1021 taatacgcat cggattgatt gcctaattta ctaatttaac agtcggcacg agaaatttga
     1081 tcgccgcaca ccaaacagct aaaaaggcct gcataaagcc tatatgctgc agcgtactca
     1141 atataaattg cagtgaaaaa tcgttacgca accaatactt ttattattga cacttctctg
     1201 ataccggtac cgaaacaaat ttaacggcac cattagccat tggaaattgg ttgaaaagcg
     1261 cggttaacat atcgggtacc accgccgata agacaccaca agcgccttca ctttgcgcat
     1321 ttatttcata caagcgagta cgttcattat tattgcgctc aagtactaca cccactttgc
     1381 gctcgtacca acctgatttg cgcgcgccgt ctaaaatcat aacattgctg ccaaaactgc
     1441 cgcgattgga tcccaccata acacctgtac gcacaccatt atcgccagtg tcagcgacac
     1501 ggctcactga ataggacaag atagcgtgta attgcgcctg ttgtggcgcc gcaagtgcaa
     1561 acccttgctt aactagcgcc cgctccaatt ggcttttata caaaccaaac tccaatgatt
     1621 gcgccaggct actctcagac ggcaccacgg caacgctagc cgaggtgctg ataggttcgg
     1681 gcgcccgaaa actattgacc cgcgtgcgca gggtttgcgc acagccgact agaaccacaa
     1741 gcaccagaat cagaaccgcg cgagctgagt aggtcattat tgtttctcca atgcgtccac
     1801 cagcgctttg aattgactga ggttggacga gttcaactgc atcagaattt tatgcgcttc
     1861 gatcacacga caacgaactt gctcctcatc cacattttca ccgtctgttg catcgagaat
     1921 cgtttctaaa ttagcgggta atttcgcttc ggaaaccatt tcaaaaatat cgttaaaacc
     1981 catggtctca agcaaacggt taatactggg attggcagtg agcaccagcg gctttttaaa
     2041 gccaaatttg cgggatgtga tcgcaatttt cgccatcagg ccaagggtag tactgtcgat
     2101 acaggttgct tcacgcaaat caaaaataac cgaccgaaat ggccgcgcat caaacatttg
     2161 ggcaataaaa ttatcaaacg aaatacacaa cgtaagccgc acatcgccca gcatccgaat
     2221 gacattgtcg ccattttggt aggccaccaa aatttgacca ttgatcataa acgcccccgc
     2281 tgctccaaac taagtatagc tatatcgtct ggcccggcac gttcggcggt taaattcaaa
     2341 cgcgccaaaa ctcctcctaa cccaccttcc aacccctgca tacgctccag caaataagcc
     2401 tcccgctcca gcagcccgtc cgccggcagg gtatctaaaa ttccgtccga gaacaccacc
     2461 agattaaaac catcagcaac ctgctcgtgg aatacttccc atcgattatc ggcaaaaatt
     2521 cccaccggtt tgcccttgcc gtctagataa cgggcgcgcc cgccctctac gaggatgggc
     2581 agaggcaaat gaccggcggt gacataactg agcactcgct catgagtatc caatacgccg
     2641 acaaaacagg ttaaatggca gccaaaatgg ctgtgtaaca actctttatt cacatactgc
     2701 accagcaagc tggggtcgcg cagcagattc acttcttctt cctggctgcg gcagtcttta
     2761 aacaggcgcc taaccagggg tttcaaccag atggtcacaa acgccgacga ggcgccatgg
     2821 ccggagacgt cagttaaata aaaagccaca tagcggtttt ggatgccgcc ataatcgacg
     2881 aaatcaccac ttaaatagag ggagggaata accttgtgtt ccaatgtgac accggccact
     2941 ttggcaggag tggccggtaa taggttgcgc tgaacttggc gagcggcctg ctgatcacac
     3001 tcgaggatac gcacataatc cctcaattct aggttggccc gctccagatc gtgcttgtat
     3061 tgttcgttct cgcgccgtaa atcgcggctt actaaggctc ggttgacggc gtgggttaaa
     3121 aaatcaatat tttggatggg cttaataagg tagtcagtgg cgcccttacg cagagcctcc
     3181 accacatcac gggcgctgcc cacggcggag gtcacaatga ccgaaaccgc attgtcctgc
     3241 tcccgcaggg ccagcatgag attaatgcca tctatatccg gcatggttaa atcgactaac
     3301 accaaatctg gccggtgagc aatgaaccac tgtaatcctc cctgacttga cgtttccaaa
     3361 tgcacgccat aatcactatc ttgcaacata gcaactatag tgtggcgaac gctctcgtca
     3421 tggtcgatga cgaggagtgt tctgaggtcg ctgatcatta atcacctata cgacggggat
     3481 aatggtgtat ctcgaagggc gctaacagta ctcctctccc ttagcccccg caagcgacaa
     3541 acagcaattg cagccccccg caacttaagc cagtttcgat aatagtgacg tggtgcaccc
     3601 aagtgtgccc aaagcggata tgggtatcat tacccacctc cagcgctgga aaaaatcacc
     3661 ctttaggcta aggtaaaggc tgtacgtcca caggcggatc atcgctaggt aaaagcctga
     3721 gtgtttttac gcaaacagtg gcgcattaaa cctaagatca tccgactggg gggtttatgg
     3781 agttaagtac gcggaattat caagaaaagc gcagtttcat tcgcatgaaa ctcgactgtc
     3841 cggccaagat tttgctggag agtgaggaat tgacaggggt ttgttccaac ctcagcggcg
     3901 gcggaatgct cttggtgctg gatgccccca ttaatctagg tactgagtgt gtcgtcagta
     3961 ttagctctca cttcgggcac ggccccatgc tcagggccaa aaccaaggtc atccgcgcca
     4021 tgtcctgcga tggcaagtgc cacgcgggtc tctctattgt ggagctgttg gacaatcagt
     4081 gacgaaaagc caggccggca tctattcaat aaagagtttc tattcacccg gaagaatgct
     4141 ggcgcggcct ccgcttgggg ccgtgctgac accagcctaa attaaaaatc cttaagatcc
     4201 gtatccgcac cgaaatcatc aattacctgc ccatccgcag cattaaaggc gcggttttgc
     4261 agaaaacctt cgcggatgaa aatataccga tccccgctga tcagctcttc cgccgccagc
     4321 agctgactgc gcttgtcgat aatttttagc gccgagagac tgttataagc gcgcaccgac
     4381 tcggtataac tcactgggtg ggtgaaaaag tccactgggg tagcaacgaa ggcttcagtg
     4441 agggtggaag gcccaagaaa tgggatcaca aaataagggc cagtggggac cccccaggtg
     4501 cgcaaggtct ggccaaaatc ctcgctgctt gtcggctttt tccagcccat catatccgcc
     4561 acatcccaca gcccaacgac tcccgcagtg ctgtttacca caaaacggcc taaatcacgc
     4621 ccggcggctt tccacttcca ctgtaaaatg tcgttaagca cgctggccgg ctccagtaaa
     4681 ttctcaaacg cattgctaat gcctgtctcc acgggagctg gtgctataaa cttgtagccc
     4741 ttcacaacgg gctttagcag ccagcgatca aaaaaatcat tgaacgcgaa ggccttgcgg
     4801 ttgtatccct cccaagggtc attggtctct tggccgcctg ctgccgcaag tgccgccggc
     4861 gcgcctgccg ccccccaaat taccaccgca actgccaacc ctgcccgacg caataaatgt
     4921 cccatgcccg ctaacctaaa gatgtgaatt tgcgcggatt ttacttgatg ctgggagtga
     4981 aaacaaaaaa gggcccgaag gccccataaa aaacactggc gagcgtttaa ttaaaaaatc
     5041 aattcgccgc caagtgcgaa gtaatcatta ccttggcttg cggtaacggt gccggttgat
     5101 ttggtttttt gccagctaac atcagcgctt tcggtagtgt aaaaaccaaa taaacgcgtg
     5161 tttttgctca gcttataatc agcacccagg cttaatacat ccgcatcacg ctcaacttca
     5221 gcgccacgca aaatgtctga ttgaccatac tgcgctttaa gtgcccattg gccgagagtg
     5281 tactgagagg aaacaatcca gccgccctgg ttttctttgc caactaacga cttgttatcg
     5341 gccgctgcgg tgtaagtggt ggcgtcgtgg ttatcgtata aagcgcccaa ttgccaagcg
     5401 cccagcgtgt attgcgcaac caagcgataa acctggcttt catcatttaa atttttatta
     5461 gactcaacca cgacaacatt tttatcaaaa gcaatagcgc cgtaaaaacc gccctgttca
     5521 taggttaacg ccagtgacgc tgcattatca cggccctttt ctttattaat agcgtcctgc
     5581 tctttgctat tgccttcgct gagaattaaa tcaatataac ctgtaaaccc gctcatttta
     5641 ggcgtggtat acataacaga atttttgcgg cgataatcat taacagtgat gtagttgttg
     5701 atatcgcctt gcaaatcgtt aaacaaatca actttatttt gtactgattt taatggcgta
     5761 tcaaaataac ccgcttgcgc ggcaccaaag ctacctttta agccaaagta aatattgcgc
     5821 tgtcggatag atgtggtatt gggatcggta ccgtctacac catcgtcggc atatacggcg
     5881 tattcagcta gataaaatcc ttccaatcct aaatcggcga atttttcgct gcctttaaag
     5941 ccaatgcgcg agacgttatt ttcaagtgta gtggtatctg acttagtggt tgttactgta
     6001 tcgcttgcac taacaaattg cagtgtttcg gtggaatttt gctgtgcggt taggttgatg
     6061 cggccataca cggtaggggc atccgccata gcacccgcag gcgccaaaca agataaagcg
     6121 atcaatgaac gcaatttaga actcatgctt tttctccagg taaaattaaa acgctctagc
     6181 agtcttatcg ctgccgttgg cgcgcagatt atgaattatt tgttactgtt atgttacaaa
     6241 accgcatgct ggccacattt ctttgcctct agcctcgcca tacgctaaaa cacagcctac
     6301 ttgccctcct cgcctataca gctcaccagc ccagttatgg tttataagac aatatgtttg
     6361 ctgttaaccc atgcatctaa caatcggagc gatccagagc cacaggaagg agcctgggaa
     6421 tagctaaagg tgtgtgtcag cagcggccag acatagattg tccagcttta agccctatgc
     6481 agacagctaa tacttggtga cacaccgtcc tgcggtgcgc cactgcattg catggtagga
     6541 atacggcgtg acaatagcgc caccgaattt tacttaacct ggaaacatat tgccatttaa
     6601 atatggtaat tagagataac gcgtaacgcc catgattaat tccgcgcaca atgcagaata
     6661 aattaagcca aatacacaaa gttgtgactt atcgcaagca ttgcacgccg cgcatgatat
     6721 aggtgagcgc taaacctgcc gggcgctgtt tcacagcatg ttaagtgaca cgtcaaaaat
     6781 acattggttt ttgcctgggc cttaccacac gccgccagcc cttatagtat taaaaacact
     6841 accaacccat ttccaatagg gcctcccagg cttttgtcaa acgtttggcg gaggctggca
     6901 cgcgggtttt taattgctgg gcaaagagcg acacgcgaaa ctcttctatt aaccagcgat
     6961 attcctgcca ggcagaattg tgaaaataga ctgcggggcc tttttgttgc cacagctgct
     7021 ggtgtttctg ccaaaaaatc agtacttctt gattgcactg tttatccttg agcggacttt
     7081 gctgggcttt ttcaaaacgt gtttgcagtg cactgatata gcgtggcatt tgttgcaact
     7141 gcaaaaaggg ggtggcaaaa ataaatcctg gataaagcca atggctgatt tgctcattaa
     7201 tagcggccac tgtatcaaac accgccaaag cgtttttggc ggttttcatt tgatttttta
     7261 gcgctaaaac ctgtgtaaat atggcctcta acacatgctc atactcaaca gctgttttgg
     7321 ttaacgcaga gcgcgccacc aataattgtg taaaagcctc ggcgttgcga ggagtatcgt
     7381 gttgactaaa ataggcctga tacgctgcgg ccaacaataa atcgtctacc acctgtgcag
     7441 gcgtacccag ccctaaagta gacagtaaaa tactggtttt tttaaataga tttttatgca
     7501 gatatttgca ggcatcgggc atagccaata gagccaagcg gctgaggccc ttggcgctta
     7561 accacgcggc ttccagagga ttatccagca atttaagatc gagactggcg ccttggtcaa
     7621 ttaaagccgg ataggcgcga atacgcactt tgccgcgcat caaggtggat tcagcttcca
     7681 aaacaccaaa atcccagcgc gtaattccag aacgctccac ctgatcaccc gccgtttgta
     7741 aactctgttg cacattggcg cggtatttaa ggcgcaaatc ggccaaatcg cggccctggg
     7801 caatcaaacg gttttgatca tccatcacct taatattcat gacataaaat ggatcaatat
     7861 caatatcggc ccatacctca gcgggcaatt tctcgccggc aaggcgctgc aattcttcgc
     7921 ttaatacctc cagcaaagag cgtttgccaa ggctaatgcg ggccaatatt ttatcgacgg
     7981 cgtctggaat gggcacaaag cgtttacgcc attgcttcgg catacattta ataagcgcga
     8041 ttagtttgtc gcgaattaaa ccgggcacca gccaatcaag ggggccctgt ggcaggttat
     8101 gcaaaatatc aatgggcaca tgtaccgata cgccatcatc cgcagcgcct ggctcaaaat
     8161 ggtaacttaa cggcaattcc attccatcca ccgctagtat tttggggaat tgcgcttcgg
     8221 ttatggcttg ggcgtcgtgg cgctttaacc gcgccatggg aataaataat aaacgcggtt
     8281 gttcccgttc gcaggtttta cgccaatgct caaaacttgc caaattggct atggtctctg
     8341 gcaaacgctc ggcgtagaag gtaaaaattt cctcatcgtc caccaaaata tccttgcggc
     8401 gggatttcgc ctccaaaccc tgtacttctt cgatacaggc gagattatgg cgataaaatt
     8461 cgccgatttt atggggtggg ttgcgcccgt agcccccttc caccaacgcc tggcgaataa
     8521 acacctcacg ggtttcctgc gggttaatat gtgcgtagtt aatcctatgt ttatctttaa
     8581 ttaccaaccc gtagagcgta atgcggtcgt aggccatgac ctgaccggtt tggtgggcgt
     8641 aatggggttg atagtagtga tgttttaaca gatgttcggc ggtttctaac agccattcag
     8701 ggtctatttt ggccaccgta tggccgtaga ctttactcgt ttctaataaa ttcgccgcca
     8761 gaatccattt tgggcgtttt ttaaattgtg aggaggcggg gaaaataaaa aatttacgat
     8821 tgcgcgcccc taaataatcc tgctcgctag gctcctcgga tttaacacca atgttggcac
     8881 ttaggcccgc cagcagtgct ttgtgtatcg cgtcgtagga ggccggttgg gtattttctt
     8941 taaaacccgc ctctttacag gctaagcgca attgatggtg taattctcgc cattcgcgca
     9001 ggcgcaacca ggataaaaat tctttttcac agagtttttt aaattgactt gcggtaagcg
     9061 cttggcgctg ctcctctata tattcccaga gcgcaaccca ggttaaaaaa tcggaatcgg
     9121 cgtgccaaaa acgtcgatgt ttttcgtcgc tggcttgttg tttatcggcg ggtcgctcgc
     9181 gcgggtcttg gttggccatg gcggtggtga taatgagcat ttccgttaaa cagccgcgct
     9241 gattagccgc caataaaata cgcgcaagtt tcgggtctat gggcagtttg gccagggcgc
     9301 ggccaaccgc cgttaattgc ccctgcggcg taaccgcgtt taactcttcg agtaatttaa
     9361 acccatcgct gattaaccgc gcatccggtg gatccacaaa ggggaatttg cgaatatcac
     9421 ccacaccaat agagagcatt tgcaaaataa ccgccgcaag attagtgcgc ataatttctg
     9481 cgtcggtaaa atcggcgcgg ttattaaaat cttgctcatc atataaacgg atacaaatac
     9541 cgctggctac ccgcccgcag cggcctgcgc gctgattggc gctggctttg gaaatagcct
     9601 caataggtaa acgctgcaat ttactgcggt ggctataacg gctgatacgg gcaaatcccg
     9661 ggtcaatgac atagcctata ccgggcacgg taatggaggt ttccgcaaca ttagtagcca
     9721 aaataatttt gcggccccgc gcctgggcga aaatgcgatt ttgttcagca ttggacagcc
     9781 tggcataaag cggcaggata attaaatcag gccggttcag cgcgcgcaga gctttagcgg
     9841 tatcgcgaat ttcgcgctca ccacttaaaa acaccaaaat atcgccagcg tgtttaggca
     9901 gctgtaaaat ttcctcagca gcactcacaa tcgcatcaat ttgatcctcc tcggtaatag
     9961 aagggcgata gcgaatttct accgggtagg tgcggccgga aacttcgatt atcggtgcgc
    10021 catgaaaatg gcgagaaaaa cgctgtagat ctatggtggc agaggtgatg ataattttta
    10081 aatccggccg cttaggcagc aggcgcttta aataccccaa aataaaatca atatttaaac
    10141 tgcgctcgtg ggcttcatca ataattaacg catcgtaacg cagcagtaag ggatcggttt
    10201 gaatttcggc cagcaatatg ccgtcggtca ttaattttat ttggctattg ggtgcggtga
    10261 ggtcgctaaa acgcacctga taacccaccc gttgccctaa gggggaatta agttccgccg
    10321 cgatgcgctc cgccactgcg cgagcggcca agcggcgcgg ctgcgtatgg ccaattagac
    10381 cgcgctggcc caagccaagg gtaaggcaga ttttaggcag ctgggtggtt ttacccgagc
    10441 cggtctcccc tgctaacacc accacctgat gagcggcaat taatgcggca atttcctcgc
    10501 gtttttcgct gatgggcaat tgggtgggaa attcaatcac ctcggtgtac gccctgcgcg
    10561 cactgagcgt ttgcgccgac tgctccagca gagcggtaaa ctgctgctgt aacctgtcaa
    10621 acggctggcc cgcttttttg cgctgttcga tagtgcgggc gatttgctgc agcttgggca
    10681 catcgactag taaacaggca gtttggtcaa aaacatcaca ctgggtcata gaactttcta
    10741 aggcacgaaa ttagccgcca attatcgcct aatctcgcct ataaacctac ggcaacggcc
    10801 caggcgtgcg ggggtgacag cgcggccatt cctcggcata ctgtatatgg atacagacag
    10861 gagagcccta tgcgccttta catcgccgaa aaacccagcc tcgggcgcgc cctggcggcg
    10921 gtgttacccg gcccgcaacg caaacaggat ggctttattc aaaccgctga tggagcctgt
    10981 gtcacctggt gtatcggcca tttattagag ttagcagagc ctgaagacta tgacgcgagc
    11041 ttaaaaacct ggcgcttgga agccctgccc tttatcccta aacaatttcg ttttaaagcc
    11101 aaacggcaaa ccgccaaaca attagccgta gttaaaaaat taattaaatc cgccaaagaa
    11161 attgtccacg tgggcgaccc ggatcgcgaa gggcaattat tggtggatga agttttgtgg
    11221 gaggcacggt taactgcggc acaattatcg caggtaaagc ggctgttaat taacgattta
    11281 aaccccgctg ccatcgggcg cgcccttacc caagtgcggc ccaacagcga tttttacgcc
    11341 ttgagccaat cagccttagc gcgcacccac gcggactggt tgtacggcct gaatatgacc
    11401 cgcgcctaca gtattctcgg ccagtccaag ggcgcgcggg gtgtgctctc ggttggccgg
    11461 gtgcagacgc cggtattggg cctggtggtg cggcgggatg agcaaatcgc cgcatttagg
    11521 ccccatgatt attatgaggt catcgctgag gtggagttta acggtacacc agcagcgcgg
    11581 gtgaatgcca agtggctgcc ctccgaagcc tgcgctccct tttgtgatac tgaggggagg
    11641 gttttatcca agcccctgtg tgaacacgtc attaaacgca ttcaaggtaa acccgccctg
    11701 gtggatgaat ttaccaccca gcgcaaaaat caaaatgctc ccttacccta cagtttatcc
    11761 agcttgcaaa ttgatgcagc caaacaatta aaattatcgg cgcaaaccgt tctagattta
    11821 tgccaatcac tctatgaaaa ccaccaatta attacctacc cgcgctcaga tagccgccat
    11881 ttaccgcagg agcattggca cgaagccgcc aatattatca acactattcg acacaatgct
    11941 cagccgcttg cggccgctgc cgcccagaca gattgcacac tgcgcagcaa a
//
LOCUS       DAOQGS010000047        11831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2512_length_11831_cov_5.407948, whole genome shotgun sequence.
ACCESSION   DAOQGS010000047 DAOQGS010000000
VERSION     DAOQGS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_11831_cov_5.407948"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKZ52_02980"
     CDS             <1..207
                     /locus_tag="PKZ52_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58762.1"
                     /translation="VICTPALLIFRRRKTHDQDTPKQQAPEAPKGKIQTGLRGRGGAL
                     GWLFVICTPALLIFRRRKTHDQAR"
     gene            191..1240
                     /locus_tag="PKZ52_02985"
     CDS             191..1240
                     /locus_tag="PKZ52_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNG58763.1"
                     /translation="MTKHANLTALALVLAALPAAGALASECTDGIAKTGDGYKHCQGL
                     YLGAGVGISKLKPRVVDLPEELDDTTDTVVPNLILGYDINDDWSIEATYADQGEATFK
                     SGAAVDYKHWALSGLYYLPSNLPGLSAYAKAGVGQLKTGVARGRPDGGPFEVEQVNST
                     QVSLGLGGEYRFSNDWGLRAEALFVDKDSSQLTLNVIKRFGRDYTPPAPKPAPAPPAP
                     VAPPAPKVLTTPECKELKSLFEQVYFAVDSAELTPASLGVLQKIATTLKPFPHVALEI
                     SAHTDSQGSDAYNLALSERRAQSVVSNLSSQGVQNLSAKGYGESRPVADNKTAPGRAK
                     NRRVEMAATGGKLCE"
     gene            complement(2027..2860)
                     /locus_tag="PKZ52_02990"
     CDS             complement(2027..2860)
                     /locus_tag="PKZ52_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58764.1"
                     /translation="MATCYHCGCSIRPNEGYRRKVQTGESTRVYSSRNPGSSYGQSFG
                     LRTLCQDCAIAIDQQHAIANQKGKIAGISAAVGLITAGITFLNIRSFIDNDTPRLIFA
                     FFILGGPGFCLYFLLLLFRNSNQGENSSSQSYASVQPESMASQISNVDLNLYIAKIRT
                     VGEKVEALGISFFGVYGLERKQENMQRLGDIIFSILPPRHHGDIDVWEDHLLKTTYNR
                     FLNEFSVATNPINFEVLLSIFPGESDESEYSYLSRLNAVVREIMPIIHPEIDVESEGQ
                     S"
     gene            3109..4182
                     /locus_tag="PKZ52_02995"
     CDS             3109..4182
                     /locus_tag="PKZ52_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNG58765.1"
                     /translation="MSHDILFKELSNDSLSFKNRIWMAPLTRCRAGLEHIPNTLMAEY
                     YAQRASAGLIIAEATMAMEGNSAFWQEPGIYNQAQIDGWKLVTDAVHAKGGKIFLQIW
                     HGGRACHPALNGGKQPVAPSAIAIDDEVHTPEGKLPQVVPKELKDDEIPAIIQGFKQA
                     AINARAAGFDGVEVHGANGYLLDEFLRDGANKRSGPYGGPIENRARLLLEVIAAVCEV
                     WPSQHVGVRISPLNSYNSMRDSDPVALTAWLAQRLNDFNLAYLHVMRSDFFQAQSGDV
                     LTPARQHYKGVLVANMGYSAEEAAAAISAGLLDAVAFGVPFLANPDLPARIKTGAPLN
                     TPDQSTFYTQGAEGYTDYPALGE"
     gene            4451..4906
                     /locus_tag="PKZ52_03000"
     CDS             4451..4906
                     /locus_tag="PKZ52_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58766.1"
                     /translation="MLAVLGQKKVYEQIPELFLPTVAGQLKSIKDETKPLSPSEKFRT
                     ISLAPTSGDWLTNGEAAYRVLNERAEFNGLSLEINKNCFGNPKEFIALGKNTIGKGSK
                     AVKLDDTEMLMWRWKDPDINMVRTLEINASQSNYSIAVKRDPDSSGAGG"
     gene            complement(4998..5306)
                     /locus_tag="PKZ52_03005"
     CDS             complement(4998..5306)
                     /locus_tag="PKZ52_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNG58767.1"
                     /translation="MPTPIHIKERLFLALAQIPAGRVITYGELAKRAGMPNGARLVGR
                     ILGELPEDGGLPWHRVINAQGKLSFPPGEQTYYRQKQLLMQEGVEFVRDKIKLSVYGV
                     "
     gene            complement(5363..5740)
                     /locus_tag="PKZ52_03010"
     CDS             complement(5363..5740)
                     /locus_tag="PKZ52_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNG58768.1"
                     /translation="MSEEQYSQQYSDEGFWQKVKTTARSAGVAVLENGLKMYYAARDD
                     KTPAWAKATLYSALGYFICPIDAIPDMLPVVGFSDDLGVLIAAAATTAAYITDEHGAK
                     AKELVKRWLPGEKSSQTDIKSAD"
     gene            complement(5808..6668)
                     /locus_tag="PKZ52_03015"
     CDS             complement(5808..6668)
                     /locus_tag="PKZ52_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNG58769.1"
                     /translation="MIIAACKYPLETLGSFSEFAHKQTLLLAQAKNQGAAIAVLPEYL
                     ALELAGFTSERGDLAASLLAIQEFWPHWQELFTRLAQQFKMWICAGTFLVADGQGRYV
                     NRAGLFSPAGTIGWQDKILLTGFEKTCQLIAPGNSLQVWQTGSIYTGIAVCYDCEFPL
                     GIRAQRQAGAQLMLVPSCTDTLAGAHRVRIGCQARALENRMAVACATTAGQLPWSPAL
                     DMNTGEAAILVPMDIGLPDNGIAAQTGGDNNWAIADVDFTALQFPAAAQVANDSDWPD
                     QITQASVQTL"
     gene            complement(6665..7270)
                     /locus_tag="PKZ52_03020"
     CDS             complement(6665..7270)
                     /locus_tag="PKZ52_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HNG58770.1"
                     /translation="MPTTKILTASGAAIAPYLADLARLRIEVFRHWPYLYEGNMDYEL
                     AYLKTYVSSPRSICVLVQAEGKIVGASTGLPLIDETAEFQAPFMCAGWDIQKLFYFGE
                     SVLLPAYRSQGLGHEFFNYRQQHAQGFNCFTHTTFAAVERPLNHPLRPTNYTDHAEFW
                     RKRGYTHQPQLAMQLSWPEIGAAEASTKTLSFWLRPEPVQL"
     gene            complement(7741..8268)
                     /locus_tag="PKZ52_03025"
     CDS             complement(7741..8268)
                     /locus_tag="PKZ52_03025"
                     /inference="COORDINATES: protein motif:HMM:NF020904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HNG58771.1"
                     /translation="MLDVQRLKHMMQTHQVASPPDLVGVCAEEIARLQARLGLTFPAS
                     YQQFLLTCGRSAGLLSPWVAFYFDDLVEIREEFNEHLGELIDPFDVPANALVFAQADA
                     IFDYFFCDGSEDPPVYRLNFQTQNPSCERCAASFSAYLEALVLASDKEQWLDDLVEDE
                     TYSEASCDGDEDNLL"
     gene            8577..9377
                     /locus_tag="PKZ52_03030"
     CDS             8577..9377
                     /locus_tag="PKZ52_03030"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HNG58772.1"
                     /translation="MASPLSVILSPMAACLSLLAANGSAAEVAWQGPNNGWVYDGLTN
                     KSDTARVAYPTPPIDRGRVRERSMIAGRLKSDLPQPHTLAVNGNPLPLYTDADGNFAR
                     PYALGPASNSLSLATSDGAQVKRVQFYDSNKSRPQARIRLVLGWDDPQAEIDMHVITP
                     DGQRAYYGDPILKEGGGLDPDGVDGPGPEIFSLASPLRGRYLVYVNYWGNLNNNGYNF
                     TAGSNRNDIITCQITLVFNEGTVDEKRENFIVPLRSIGQLQLVKEFNY"
     gene            complement(9561..10091)
                     /locus_tag="PKZ52_03035"
     CDS             complement(9561..10091)
                     /locus_tag="PKZ52_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58773.1"
                     /translation="MASRQDIVNQALWEANYDHWKPTDDEMAEIFTTAGLNAPSKSDL
                     KKFRTAPGNISINGQSIAWCGIFATYVLKKWGGIDVKWSNGGIVGSGVNKTWNRDGMR
                     PGDVAVVRGKLDSKGRGVHHHFIITAIDYAANTMESVDGNSTNNEIVWHTNKHISYTG
                     ADKDSYTPYCHYKLKI"
     gene            10420..11466
                     /locus_tag="PKZ52_03040"
     CDS             10420..11466
                     /locus_tag="PKZ52_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58774.1"
                     /translation="MIIRQANIQLAARHSYQELREVRERLTVRTGAANGQGQNTGPVQ
                     VETPLMQAAPPAASAPVLGARQSQTLDLRGAASANERLQLMIIARLYQQITGEELQIL
                     TPDQLQAAAASVSLEIPTAPVAPSGPALVYERQTRSTEVEKMHFAAQGQVVTQDGKSI
                     SFNAELFMSRSFTNTTSLTLIDGQPVMKDPLVINFDGTGAELERTRFAFDLDSDGTEE
                     QIANLRPNSGYLALDKNGDGVINNGRELFGPTSNNGFAELAAYDEDNNGFIDEGDSVY
                     SQLRIWQRDSNGASRLLALGDRQIGAIYLGHAMSPMQLKAADNTSLGEVVSSGVYLRE
                     DGSTGLVQQINLAV"
BASE COUNT         3010 a         3275 c         2917 g         2629 t
ORIGIN      
        1 gtgatctgta cacccgcact tttaatcttc cgcagaagga aaacccatga ccaagacacc
       61 cccaaacaac aagcgcccga ggcccccaaa ggcaagattc aaaccggctt aagaggccgg
      121 ggcggcgccc tgggctggtt gtttgtgatc tgtacacccg cacttttaat cttccgcaga
      181 aggaaaaccc atgaccaagc acgctaatct caccgccctc gcccttgtcc ttgcggccct
      241 cccggccgca ggggctttag cctcagagtg tacggacggc atagctaaaa ccggcgatgg
      301 ctacaaacac tgccaaggcc tttacctggg cgccggggtg ggcatcagca agctcaagcc
      361 acgggttgtg gacctgcccg aagagctgga tgacaccacc gatacagtgg tgcccaacct
      421 gattctcggc tacgacatca acgacgattg gagcattgaa gccacctacg ccgaccaggg
      481 cgaggccaca ttcaaatccg gcgccgccgt agactataaa cactgggcct taagtggttt
      541 gtattacctg cccagcaatt tgcccggcct gtctgcctac gccaaagccg gtgtcggcca
      601 gctgaaaacc ggcgtcgccc gcggccgccc agacggcggc ccgtttgagg tagagcaggt
      661 caacagcacc caagtgagcc tgggcctggg cggtgaatac cgctttagca acgactgggg
      721 cctgcgcgcc gaagccctgt ttgtggacaa agactcaagc cagctcacct taaatgtgat
      781 caaacgcttt ggccgggatt acaccccacc cgcccccaaa ccagcaccgg caccgcccgc
      841 ccccgtagcg ccgccagcac ctaaggtact caccaccccc gagtgcaaag agcttaagtc
      901 cctgtttgag caggtctact tcgcggtgga ttccgccgag ctgaccccgg cctccctggg
      961 cgtgctgcaa aaaatcgcca ccaccctaaa acccttcccc catgtagcgt tggaaatctc
     1021 cgcccacacc gacagccaag gctccgacgc ctacaacctg gcgctctccg agcgacgggc
     1081 acaatcggtt gtgagtaacc tgagcagcca gggggtacaa aacctctctg ccaagggtta
     1141 cggcgagagc cgcccggtag cggacaacaa aaccgccccc ggccgcgcca aaaaccgccg
     1201 cgtggaaatg gcggccaccg gcggcaaact ctgcgagtaa gcccctcgcc ggacaccccg
     1261 acttccccct tggaaagggg gagccaaaac taaggcactt tgcttaaatt taggtatctt
     1321 ttctctcccc aaatcgcata ttgcaagagc aatgcgcaaa gcgaaactgc ctgtaggcaa
     1381 atcacaagga agtgatttgt gcgcgggagg gttcgttcca caacaaccct attaatactt
     1441 aacgccttaa atctccgcct ccaggctcca ggctccactg atcctgcgac caaaatcttt
     1501 aagtgacaca ggtatctaga gacaaccctc gtgttgcccg gcttttacct tggctaccca
     1561 tcgccgagtt cagtggcggt ttttgaaccg tagttttatt ggatactttt tcgcagcaga
     1621 agcataacac cacgacttaa acagattcta agtgccgaag cggtgctgca acgatttgtt
     1681 agatatttaa atttacactg catccgaata ttcaattatt ggactaagcg tactaattca
     1741 atcaggcttt gtagcaaagt aacttcttgc atcctgatct gctctcttta gccacaagag
     1801 aaaatcagaa gccattgcgt tgtttttgaa aaagacgtgt actccatttg gggaaaaagt
     1861 aaagctatct ggaatttggt ctgagcttgc atgtatgagg cacagaggac aaagctccac
     1921 gtaaaactga aactttgcaa catcaaaaac aagtgaatat gtttctatct ctccgcaatt
     1981 attggatttt tattagatgc acttatgtag aaatgaatag ttatcttcat gactgcccct
     2041 cagattcgac gtcgatttcc ggatggatta tgggcattat ttctctcacg acagcattca
     2101 gtcgagatag atatgaatat tccgattcat ccgactctcc tgggaatatc gataagagca
     2161 cttcaaaatt aatgggattt gttgcgacag aaaattcatt taagaagcga ttataggttg
     2221 tcttcagaag atgatcctcc caaacatcaa tatccccatg atgccgaggt gggagaatac
     2281 taaaaataat atcgccgagg cgctgcatat tttcctgttt tctctctaac ccgtagaccc
     2341 caaagaaaga tatgccaagg gcctcaactt tctcaccgac agtgcgtatc tttgcgatgt
     2401 ataaattcaa atcaacattt gagatttgtg acgccatact ctctggctga acagacgcgt
     2461 aagattgact tgatgaattt tcgccttggt tagagtttct gaataacaaa agcaagaaat
     2521 acaagcaaaa tcctgggcct cccaaaatga aaaaggcaaa tatcaatcga ggggtatcat
     2581 tgtcaataaa tgaacgaata ttgagaaatg tgataccagc ggtgatgaga ccaacggctg
     2641 cggaaatacc tgctattttc cctttttgat tggcaatcgc gtgctgttgg tcaatagcga
     2701 ttgcgcaatc ttggcaaagt gttcgaagcc caaaggactg accatatgaa cttccgggat
     2761 tccgcgaaga ataaacgcgt gtggactctc ccgtctgaac tttccgacga tacccttcgt
     2821 ttgggcggat agaacatccg cagtgataac aagtagccat tttttcccta gtaataatgg
     2881 aggatgagcg ggcagactaa caggaatcac acaggcgtcg ataccccaaa aaggcgcgcc
     2941 taaccgtcgc ctatgggaag aaattctcaa gacaactccc cccttgaatc ccccacttcc
     3001 tcctccatca tgctctaacc ctgcagcccc acagacgcag atccaaccac tcagcccctg
     3061 cgtaagcggc ccaaccccct agccaataga caacccagag acacgcccat gagccacgac
     3121 atattgttta aagagctaag caacgacagc ctctccttca aaaaccgcat ctggatggcc
     3181 cccctcaccc gctgccgggc gggccttgag cacatcccca ataccctgat ggccgagtac
     3241 tacgcccagc gcgcctcggc gggcctaata atcgccgagg ccaccatggc catggagggc
     3301 aactccgcct tctggcagga acccggcatt tacaaccaag cgcagattga cggctggaag
     3361 ctggtgaccg acgcagtgca cgccaaaggc ggcaagatct tcctgcagat atggcacggc
     3421 ggccgcgcct gccacccggc cctcaacggc ggcaaacaac ctgtggcccc cagcgccatc
     3481 gccatagacg acgaagtgca cacccccgag ggtaaattgc cccaagtggt acccaaagaa
     3541 cttaaggacg acgagatccc cgccattatc cagggcttca aacaggccgc cattaacgcc
     3601 cgcgccgcag gcttcgacgg cgtagaagtg cacggcgcca acggctattt attggatgaa
     3661 ttcctgcgcg acggcgccaa caagcgctcc ggcccctacg gcggccccat cgaaaaccgc
     3721 gcccgcctat tgctagaagt aatcgccgcc gtctgcgagg tgtggccgag ccagcacgta
     3781 ggcgtgcgca tctccccgct caatagctac aacagcatgc gcgatagcga tccggtggcc
     3841 ctcactgcat ggctcgccca gcgcttaaac gactttaacc tcgcgtacct gcacgtgatg
     3901 cgcagcgact tcttccaagc ccaaagcggc gatgtactca cccccgctcg gcagcactac
     3961 aaaggcgtat tggtggccaa tatgggctac agcgccgaag aagccgctgc cgccatcagc
     4021 gcaggcttgc ttgacgccgt cgcctttggc gtgcccttct tagccaaccc agacctgccc
     4081 gcccgcatta aaaccggcgc gccgttaaat acccccgatc aaagcacttt ttacacccaa
     4141 ggggcagagg gttacacgga ttatccagcg ctgggggaat aggtattgtt aataggcgca
     4201 ttaattaaat tggagtaaat gtgaaaaggt ttgtgtacta atagatgggc acaaaccttt
     4261 atattaaaaa ggcaaaaact ctcgggcatg aatgaatatg cgcaacacaa aggtgtttac
     4321 tccccttagc attgttttgg gctttctaat ttccgctatt tgcagcgcgc aacaaccgcc
     4381 taccgcaaac acaaaacagc cagagtcgcc aacaatacct gcagcaacaa aatgcccggc
     4441 tgtagctgag gtgctcgccg tcctcgggca gaaaaaagtt tatgaacaaa tacccgaatt
     4501 attcttaccc accgtcgctg gccaattaaa atcaattaaa gacgaaacca aacccttaag
     4561 cccaagcgag aaattccgca ccatcagcct cgctcccact tctggcgatt ggctgaccaa
     4621 tggcgaggcg gcctaccgag tattaaatga gcgcgccgaa tttaatggcc tatcactgga
     4681 aattaataaa aactgttttg gcaacccaaa ggaatttatt gcccttggca aaaacactat
     4741 aggcaaaggc agcaaagcgg ttaaattaga cgacacagaa atgctgatgt ggcgctggaa
     4801 agacccggat attaatatgg tgcgcacatt ggagataaac gcgagccaga gtaattattc
     4861 catcgccgtt aaacgagacc ctgactccag cggcgcaggc ggttaacgct ctccctacct
     4921 tctatttagt tgggccttag tgcccaactt tatgcatcct tgactcgtca acagaaaacc
     4981 gtgcttctga tgtgttttta aaccccataa acagacaatt taattttatc ccgcacaaac
     5041 tccacccctt cctgcattaa taattgtttc tgtctgtaat aggtttgctc ccctggcgga
     5101 aacgacaatt tgccctgagc gttaattacc ctatgccagg gcaaaccacc atcctccggc
     5161 aattccccca aaatcctccc cactaaacgc gcgccattgg gcattcctgc gcgcttggct
     5221 aattcaccgt aggtgatgac gcggccggcc ggaatttggg ctaaggcgag aaaaagcctt
     5281 tcttttatat gaataggtgt aggcatgcta attaaaaaat caaatactgg attaaatcag
     5341 agtgtgacaa atagatacag ttttaatcag ccgattttat atctgtctga ctagactttt
     5401 cacccggcaa ccagcgctta accaattctt tcgcctttgc accatgctca tcagtaatgt
     5461 atgccgctgt agtggcggcc gctgcaatta aaacacccaa atcatcacta aaccccacca
     5521 ccggcagcat atcgggtatg gcatcaatgg ggcagataaa ataacccagg gcgctgtata
     5581 aagtggcttt tgcccaggca ggcgttttat cgtccctggc ggcgtaatac atttttaagc
     5641 cattttctaa aactgcgacg cctgcgctgc gggcggtggt ttttactttt tgccaaaaac
     5701 cttcgtcact gtactgctga ctgtattgtt cttccgacat aaaagcccca aaaaaatatt
     5761 aagaataatt caagaaatca taatctaggc tgcatacgcc tgtatattca cagcgtttga
     5821 acactcgcct gggtaatttg atcgggccag tcggaatcgt tggccacttg tgcggcggcg
     5881 ggaaattgca acgcggtaaa atccacatcg gcaatggccc agttattatc accaccggtt
     5941 tgcgccgcta tgccgttatc cggcaggcct atatccatgg gcactaatat cgccgcttcc
     6001 ccagtattca tatccagcgc tgggctccag ggtaattgcc cggcggtggt ggcgcaggct
     6061 acagccatac ggttttccag ggcgcgggcc tggcagccaa tgcgcacccg gtgggcgcca
     6121 gcgagggtgt cggtacagct gggcaccagc attaactgcg cacccgcctg ccgctgggcg
     6181 cgaataccaa ggggaaattc gcagtcgtaa cacacggcta tgccggtata aatcgagccg
     6241 gtttgccata cctgcaatga attgccaggc gcaattaatt gacaggtttt ttcaaagccg
     6301 gttaacagaa ttttatcctg ccaaccaata gtgcccgccg gtgaaaataa tccggcgcga
     6361 ttaacataac gcccctgccc gtccgccact aaaaaagtac cggcgcagat ccacattttg
     6421 aattgttggg ccaggcgggt aaataattct tgccagtggg gccaaaattc ttgaatagcc
     6481 agtaatgatg ccgccaaatc gccgcgctcg gaggtaaaac ccgccaattc caaagctaaa
     6541 tattctggca ataccgctat ggcggcgccc tggttttttg cctgggctaa taataatgtt
     6601 tgcttgtgtg caaactcact aaaactgccg agggtttcta gcggatattt acaggcggca
     6661 ataatcatag ctgcaccggc tcaggccgca gccaaaaact taaggttttt gtgctggcct
     6721 cggcggcgcc gatttccggc cagcttaatt gcatagctaa ttgcggctgg tgggtatacc
     6781 cgcgcttgcg ccaaaattct gcgtggtctg tgtaattggt tgggcgcagc ggatggttta
     6841 agggccgctc cactgcggca aaggtggtgt gggtaaagca gttaaagcct tgggcgtgct
     6901 gctggcgata attaaaaaat tcgtggccca ggccttggct tctatatgcg ggcaataaaa
     6961 ccgattcgcc aaaataaaac agtttttgta tatcccaacc ggcgcacata aacggcgctt
     7021 gaaattctgc tgtttcatca attaaaggca aacccgtgga ggccccgaca attttgccct
     7081 ccgcctgcac taatacacaa atactgcgcg gggatgatac ataggttttt aaatatgcaa
     7141 gctcgtagtc catattgcct tcatagagat aaggccagtg gcgaaatact tctatgcgca
     7201 ggcgggcgag gtcggcaaga tagggcgcga tagcggcgcc tgaggcggta aggatttttg
     7261 tagtaggcat ggcgataagc taaacgggag ggcgcataac cttaacagtt tggggaacgc
     7321 ttaagcaaaa cccgttaaag cgggggctct aaactggatt cattgggctc attataagta
     7381 actattcagc acctttttac tttagctggg cgcatattgc cgcactgact ggaaactatc
     7441 aggcaagagc tttgcgagtc cttatactgc gcctgcagtg atgaatacag gttattgcag
     7501 acacgccgta aatacatcca tcatataggc tcgttaaaaa ggtccctctt tttgacggcc
     7561 tgaaataacc tttattcacc acgactgcac cgcgaatcga tactgccgat tggccaaacc
     7621 cgccaaaatt tatcgcccgc gcttaagaag gtaacaaggc gtgctgaata gttattaata
     7681 tatttaacat gcgcccatat gcgtgcaaca ggaattactt aagcaattag cctctaacgc
     7741 ctacagcaaa ttatcctcat ccccatcaca actcgcctca gaataggttt catcctccac
     7801 caaatcatcc aaccactgct ctttatcaga agccagcacc agggcttcta aataggcgct
     7861 aaaacttgcg gcgcagcgct cacagctggg gttttgggtt tggaaattga ggcgatatac
     7921 cggcgggtct tcgctgccgt cgcaaaaaaa ataatcaaaa attgcatcgg cctgggcaaa
     7981 tactaaggcg ttagcgggca cgtcaaacgg gtcgattaat tcgcctaaat gctcgttaaa
     8041 ttcttcgcga atctccacta aatcgtcaaa ataaaacgcc acccaaggag acaataaacc
     8101 ggcggaacgg ccgcaggtga gtaaaaattg ttggtagctg gcggggaagg taagccctaa
     8161 acgcgcctgc aggcgggcaa tttcctccgc gcatacgccg actaaatccg gggggctcgc
     8221 cacctgatgg gtttgcatca tgtgctttaa acgctgtaca tcgagcacgc actacctctc
     8281 gttaggggca ccagccatag attgttaaac taaaaggcct aaaacaaagc cgcgaggtta
     8341 aatagtaaca acgacaccta ggacatagta ggagctatgc caaaattaga ccaataggat
     8401 ttagcgatta gcagccacgg gcagataact tgcggttgcg cacagtttgc tcgtccgggc
     8461 ttatagccct taaaaacctc gatatgcggt gggccagcca gtgctaacac cttatcctta
     8521 gctgctatgc ttggccccta actttttccc catcggctat tacagaagga attcccgtgg
     8581 cctcacccct aagtgtcatc ttgtccccca tggcggcctg cttaagcctg ctcgccgcca
     8641 acggctctgc cgctgaagtt gcctggcaag gccccaacaa cggctgggta tacgacggcc
     8701 tcaccaataa atccgacacc gcccgcgtcg cctaccccac cccgcccatc gaccggggta
     8761 gggtgcgcga gcgcagcatg attgcaggcc gccttaaaag tgatttgccc cagccccaca
     8821 ccctggccgt taatggcaac cccttgccgc tctacaccga cgccgacggc aattttgccc
     8881 gcccctacgc cctcgggccc gcctccaaca gcctaagcct tgccaccagc gacggcgccc
     8941 aggtaaagcg ggtgcagttt tacgacagca ataaaagccg cccacaggcg agaattcgcc
     9001 tggtgttagg ttgggacgac ccccaggcgg aaattgacat gcacgtcatc acccccgacg
     9061 gccaacgggc ctactacggc gaccccattt tgaaggaggg cggcggcctg gacccagacg
     9121 gcgtagacgg ccccggccca gaaattttca gcctcgcctc acccttgcgg gggcgttatt
     9181 tggtgtacgt caattactgg ggcaatttaa ataacaacgg ctacaacttc acggcgggca
     9241 gcaaccgcaa cgacattatt acctgccaaa ttaccttggt gtttaatgag ggaacggtag
     9301 atgaaaagcg cgaaaacttt attgtgccgc tgcggagtat tgggcagttg cagcttgtta
     9361 aggaatttaa ttattaatta ctcaggcacc aatcgtttat agaccttgtt attggtgcac
     9421 cgtatggatg aataagggct ggcggccaaa atgcgtcagc gaatatttaa cggcgtgttg
     9481 aataggtggc agcgccgatt aaaactccag cgcgccattc aaccttgccg atcaattatt
     9541 cactgccaca aaaaacaggg tcaaatcttc aacttataat ggcaatacgg cgtgtaagaa
     9601 tccttatctg caccggtgta agagatatgt ttgttggtgt gccaaacaat ttcattattg
     9661 gtggaattgc cgtccactga ctccatggtg ttagccgcat aatctatagc ggtgataata
     9721 aaatggtggt gcacgccgcg ccctttagaa tcgagtttgc cgcgcaccac ggctacgtca
     9781 cccggacgca tgccgtcgcg gttccaggtt ttattcacgc cggaacccac aataccgccg
     9841 ttggaccatt tcacatcaat accgccccat ttttttaata cataggtggc gaaaataccg
     9901 caccaggcga tgctttggcc gttaatggaa atattgcccg gtgccgtgcg gaattttttt
     9961 aaatccgatt tgctgggggc attgaggcct gcggtggtaa aaatttctgc catttcatcg
    10021 tcggtgggtt tccagtggtc gtagttggcc tcccacagtg cttggttaac tatgtcttgt
    10081 cgtgatgcca tggtgcactc caattaatta gggaaaagaa agctcggcac ggggatgcct
    10141 aacgggttct acgcaggcgt aaggcgcggc aatcttaccg aaattctacc ggctcggtgt
    10201 gtatcaaacg ggttttatcc ctcatttatt gacctcgcat aaaaagccgc cgggtcaata
    10261 aaatacgcgg gcaaattagt gtggcgaacg cacaagccac tgcacccttt gcggccatag
    10321 aaccctttgg cgcccaaatc atccctcttg cgcccaatca gccttaaatc ccaccgcgcg
    10381 ggcgccgata acaggccatg taaccacggg tgtcctgcca tgatcattcg ccaagcaaat
    10441 attcaactgg ccgcgcgcca cagttaccag gagctgcggg aggtgcgcga acgcttaacg
    10501 gtgcgcacgg gggctgccaa tgggcagggc caaaatactg gccctgtgca ggtggagacg
    10561 ccgctgatgc aggctgcccc acctgccgcg agcgcgccgg tattgggggc gaggcagagc
    10621 caaacgcttg atctgcgggg ggcagccagc gccaatgagc gtttgcagtt gatgatcatc
    10681 gcccgcttgt atcaacagat taccggcgag gagttgcaga ttttaacgcc ggatcagctg
    10741 caggcagcag cggccagcgt cagcctggaa atccccaccg caccagtagc ccctagcggc
    10801 ccggcgctgg tgtacgagcg gcaaacccgc tcgaccgagg tggaaaaaat gcactttgcc
    10861 gcccagggcc aggtggtgac ccaagatggt aaaagcatta gctttaacgc cgagctgttt
    10921 atgagccgca gctttaccaa cactacgtcc ctaaccctga tcgatggcca accggtgatg
    10981 aaagacccgc tggtgattaa ctttgatggc accggtgccg agcttgaacg cacgcgcttc
    11041 gcctttgatt tggacagcga cggcactgag gagcaaattg ccaacctgcg ccccaatagc
    11101 ggttatttgg ctctggataa aaatggcgat ggggtgatta acaacggccg cgagctgttc
    11161 ggccccacca gcaataacgg ctttgccgag cttgctgcct atgatgagga taacaacggt
    11221 tttatcgatg agggcgactc ggtgtacagc cagctgcgca tctggcagcg cgacagcaac
    11281 ggcgccagcc gcctgttggc cctgggcgat aggcagatcg gggcgattta tttaggccac
    11341 gccatgagcc ccatgcagct aaaagcggca gataacacaa gcttggggga ggtggtgtcc
    11401 tccggggttt atctgcggga ggacggcagt acggggttgg tgcagcagat taatttggcg
    11461 gtttaggggg cttggtggcc gtctggtatt taattggccg tcgttggagg catgacaatt
    11521 agtaaaggat tgcctgccat ttctgtagcc tggatggagc gtagcctaat ccgggatttc
    11581 tggcggatcc cggattaggc tacgctccat ccaggctacc cagatagttt gatcccaaat
    11641 ttgtccctct attccttacc ccatcgtgca aagcaatgtc actaattaag cgttacggag
    11701 cattttttgg ctgcctatta gtttaaaccc cactccagcc ctccccttta aaaggggagg
    11761 gagcaagatc gcctgaagcg ccagcaaaat accttggttt gaggcttttg gcgcctcccc
    11821 cttttaaagg g
//
LOCUS       DAOQGS010000048        11770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2531_length_11770_cov_5.018779, whole genome shotgun sequence.
ACCESSION   DAOQGS010000048 DAOQGS010000000
VERSION     DAOQGS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_11770_cov_5.018779"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2255
                     /locus_tag="PKZ52_03045"
     CDS             138..2255
                     /locus_tag="PKZ52_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNG58775.1"
                     /translation="MSNLSSLQVPNLAAAEVAAWRQALSIALTAPSCAALLSLWLPMP
                     LALTGAGLVCLLLGGQWLLARTEGDQPRILLSSQWSLLASLLICLAVIWWQGPMAALA
                     AALSLGTGWAWFGYERAFMAQLLSSGLSLLLALMLGQDEQLALLGFIYLAGLWLCLLP
                     PQPAATPPSGSLLKPTKTQLIFWGSRIPAPLLLLALSTLLFLLSPRLQGAAWLPMKTT
                     APLQLGGGQAIANREFLPTRNRLQQEWLLTVAADTQSPAAGDFAAFGGRFSLEQLPAQ
                     AASGPVLARVKSPQALNLQVSRFDYFDGSQWINSGGQAEAVPIPQTGLSLSADSRLQL
                     AISWLQPSSSALAVPGGWQFLQAPTAALLLGAQSLALPQIPPAGFSYQVGSHGLSLDG
                     HSIAQRDDLLHPNYLAVPEALSGPLQDWLRTNLPTLAEPWQKALWLEHHLQTRYGANR
                     AAVLPSYKRDPVLFYLNEQGAGRSETAASALTLLLRSLGIPARLSCGWALQQRNPFTG
                     LFDLTAANAHCYSEAHINNHGWVELEPSSLFAARPSAGRLSLGVIEWQRTLTSPWRAL
                     LTGFSTLALIALFAAVLWRLWPTVQKSPALIRWRLKRLAKTRLTSDDPAAESLANLIN
                     ACKLIGYQFPPGSGIYPWACLWQTLLPSFDAERYCADFYHHHFGLNTQGDICTHHQTL
                     LKHLCELPWPELTRWVTPHTQAA"
     gene            2445..3299
                     /gene="rpoH"
                     /locus_tag="PKZ52_03050"
     CDS             2445..3299
                     /gene="rpoH"
                     /locus_tag="PKZ52_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNG58776.1"
                     /translation="MGTYLKTLDMLAPGQNLNAYVQAVNGFSVLSAEEEKALAEELYY
                     HQNLEAARKLVMANLRFVIHIAKSYAGYGLAQSDLVQEGNVGLMKAVKRFNPEKGVRL
                     ISFAVHWIKAEIHEFILRNWRIVKIATTKAQRKLFFNLRGQKKRLGWLSYDEAKAVAQ
                     DLNVEVKHVQEMEGRLFASDAAFDAGVDEDDDTAYQAPVNYLEDRRFDPAAMLEDSDW
                     ETSSVNSLEVAIEQLDERSRDILQRRWLNEDKATLHDLAAHYGVSAERIRQLEKNAMK
                     KVRTMMEA"
     gene            complement(3815..4297)
                     /locus_tag="PKZ52_03055"
     CDS             complement(3815..4297)
                     /locus_tag="PKZ52_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNG58777.1"
                     /translation="MAIYLIYEGIKGNVTADGYKDHIQISSLQFGVGRGVSMEPGNLA
                     NREATRPSISEVTITKAADNSVTDFFRESVVGSAGKTAKFKFVQTGTDKVVEYMNIDL
                     FDTIVSGYSISASSDGDPVESISISFSKIEVNYNDFDTANKNSNPQRVGYDLATAKPI
                     "
     gene            5286..6611
                     /locus_tag="PKZ52_03060"
     CDS             5286..6611
                     /locus_tag="PKZ52_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNG58778.1"
                     /translation="MTIKTSSLHFSALSKGLLKAVALATTLAAATAQANVPAISVSGK
                     QVLFDGKAGSIAGHSLFWSNTGWGGEKYYNASVVSYIKNTYKSQLVRVAVGVEGSGSL
                     VTGSAATATAPATQPDPSNKTRAFAVIDAAIANDMYVIVDWHTHNAEKYQSQAISFFR
                     EVASKYGTNNHIIYEVYNEPLQVSWSNVIKPYAEAVIGEIRAIDPDNLIIVGTPSWSQ
                     NVDEAAQNPITRFKNIAYTLHFYAGTHKSELRAKAQTALDRGIALFVTEWGAVNADGD
                     GAVNSSETNTWMNFLRSNNISHANWALNDKKEGASFLNVGASATGGWNDSNLTESGRL
                     VASLIRGWPGVSTTNSSSSSSSSSSSSSSSSSSSSSTGRPSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSGGDTGAVAGALGWLEGLSLLLLAPLAWRRKARA"
     gene            complement(6664..7599)
                     /gene="epmA"
                     /locus_tag="PKZ52_03065"
     CDS             complement(6664..7599)
                     /gene="epmA"
                     /locus_tag="PKZ52_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HNG58779.1"
                     /translation="MFASTASISLLKKRAQLLQHLRAFFYARNVLEVDVPSLGAFTVT
                     DPNLDAISCQVGSNIRYLQTSPEFFMKRLLAMGSGDIFYLGKAYRQDEQGRRHRPEFT
                     LLEWYREGFTDEDLIAETLALLSELSPGTPIQRIDYGALFSECTGLNPHRACAEELET
                     FARARLQVDFSNEPVHFWLDLIFSYLVEPNLADGLVVVEDYPLCQAALARVMPNRRGD
                     KVAKRFEIFWDRLELANGYWELTDACEQARRFAADNLSRQTQHKPLIEPDNRLLAALA
                     EGIPDCAGIALGVDRLLMRLSGCDDIAQVMPFADA"
     gene            complement(7606..8175)
                     /gene="efp"
                     /locus_tag="PKZ52_03070"
     CDS             complement(7606..8175)
                     /gene="efp"
                     /locus_tag="PKZ52_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNG58780.1"
                     /translation="MASYSTNDFRGGLKVMLDGDPCSIIENEFVKPGKGQAFNRVKLK
                     NLKTGRTLEKTFKSGDTLEGADVVDRDMQYLYNDGEFWHFMEVETFEQVQADAAAVGD
                     SIKWLKEQDNCTVTLYNGSPLAVAAANHVELEIIETDPGLRGDTATGGTKPAKLSTGA
                     VVKVPLFLSTGEVIRVDTRTGEYLGRVNK"
     gene            8227..9231
                     /gene="epmB"
                     /locus_tag="PKZ52_03075"
     CDS             8227..9231
                     /gene="epmB"
                     /locus_tag="PKZ52_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HNG58781.1"
                     /translation="MIPRTIATWHIKSWQEELSQLVRSPADLFARLNLDPALLPAANK
                     AAQAFPVRATESYLARIKPGDPNDPLLRQILPLDAELSSPADYSRDPLHELESNPVPG
                     LVHKYAGRVLLVASGACAINCRYCFRRHFPYEANTPGREDWQAALNYIRADTSLEEVI
                     LSGGDPLAVSDKQFAWLLQQLDAIPHVSRLRIHSRLPIVLPSRISPEWVDIVRSSRLD
                     KVLVIHANHPQELDASVQAALGRLAAIGVMLLNQSVLLAGVNDEAPVLADLSRTLFAF
                     GVLPYYLHLLDKVDGAAHFAVPDEKAAELKQALMQTLPGYLVPRMVREIAGASSKTPF
                     "
     gene            9552..11186
                     /locus_tag="PKZ52_03080"
     CDS             9552..11186
                     /locus_tag="PKZ52_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58782.1"
                     /translation="MTDTFDPLLMLRSLGLPEADATQLNFCGGARQAKVAQWVDDLKA
                     TQVSQTGVALYNALPEVLRLKADPLAKYEILETLRPAVQQCIAGLALTYLHQPVTLSE
                     SAQKTAIVAQALEKHLIDGYSQVVLELASANKSNNYGRHVFICAVHRALTSLGLLFCR
                     SFQLYTQVPRGLWRHLHSLYRTAEAYDLDDLTANDPILGAPLLSVREAYLRVLAAGVA
                     RLNQVAPMEAMRVFHALGNWAGQIRLEPMVADDPNVAFWLNLDADRPPLGRNRYLKTQ
                     GGDVRSLDFSRILDQLQRLASAAPEAGRSSDDLPISLLNHLCQAWEGETHRDMDRRGT
                     AVMAKVAVGLFECHYQLAGERTFDEFMQEPEDIEEVEVTLPSFANLSSKNDQKEQAHT
                     GLGQVFDVQIINQSSGGYCLQWQADTEARLEAGDLIAIREQGRRSWNLCMVRWLRRLK
                     DGPQVGLKRLANNPQPIALSARLDDGDYCPFMRGFWLPGTQAPGGKTVLAPSLPFQAG
                     HNIRVKVDGNITTGRLDQCTGGSAKVRHFTVSMNSG"
     gene            11303..>11770
                     /locus_tag="PKZ52_03085"
     CDS             11303..>11770
                     /locus_tag="PKZ52_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58783.1"
                     /translation="MIPATLRLLIIHDSRSEAERLISLLRNAGRQVRAQWVESEEALV
                     KLLQEASWDLLVVGDTAKVLSASDAIKQVRRLARDVPVILLTEEGSATTVVDGLKLGA
                     ADVVCMDEDQHLLLVINREVTNREERQGRRYAERRLKEVERRNQQLLVAPFDFF"
BASE COUNT         2658 a         3493 c         3104 g         2515 t
ORIGIN      
        1 gatcctctgg cagttgctgt tcgatgaaga acgctttgtg caccccttaa gccgcagccg
       61 ccactttcac cggcgcttaa gcgcctacca ctggcagtgg gtaatcgaac cgcaacaacc
      121 cttagcgcgg ctgttttatg tccaacctta gcagcctgca ggtgccgaat ctggccgcag
      181 ctgaagttgc cgcctggcgc caagccctca gcatcgccct tacagcccca agctgcgccg
      241 ccctgttaag cctgtggctg cccatgccct tagccttaac cggtgcaggc ttagtttgcc
      301 tgctgctggg cgggcaatgg ctgctggccc gcacggaagg ggatcagccg cgcatcctgt
      361 taagctccca atggagcctg ctggccagcc tgcttatttg cctggcggtt atctggtggc
      421 aggggcccat ggccgctctg gccgccgctt taagcctcgg caccggctgg gcctggttcg
      481 gctacgagcg ggcctttatg gcgcaactgc tcagcagtgg cttaagcctt ctgcttgccc
      541 tgatgcttgg gcaggatgag cagctcgccc tgctgggttt tatttacctg gccggcctct
      601 ggttatgcct actgccgcca cagccagctg ccacgccccc atccggctca cttctaaagc
      661 ctactaaaac ccaactgatc ttttggggca gccgcatccc ggcaccgcta ctactgctgg
      721 cgctgagcac gttgttattt ctgctcagcc cccgcctgca gggggcggcc tggctgccca
      781 tgaaaaccac cgccccccta cagctggggg gcgggcaggc cattgccaac cgcgagttcc
      841 tgcccacccg caatcgcctg cagcaggagt ggctactgac agtggctgcg gatacacagt
      901 cacctgccgc tggcgatttt gccgccttcg gcgggcgctt tagccttgag caattgccag
      961 cacaagccgc cagcggccca gtgctggctc gggtgaaaag cccccaagcg ctgaacttgc
     1021 aagtcagccg cttcgattat ttcgacggca gccagtggat caacagcggc gggcaggccg
     1081 aagccgtccc cataccgcaa accggcctga gcctgtcggc agacagccgc ctgcagctgg
     1141 caattagctg gttgcagccc agctcaagcg ccctggcggt gcccggcggc tggcaatttc
     1201 ttcaggcgcc cacggccgcg ctgctgctgg gggcacaaag ccttgccctg ccgcagattc
     1261 ccccagccgg ctttagctac caagtcggca gccacggatt atcactagac gggcacagca
     1321 tcgcccagcg ggacgacctc ctacacccca actatctcgc cgtgcctgag gcactgagcg
     1381 gcccgctgca ggactggctg cgcaccaacc tgcccaccct tgccgaaccc tggcaaaaag
     1441 ccctctggct ggagcatcac ctgcaaaccc gttatggcgc caatcgagca gctgttttgc
     1501 cctcctacaa gcgcgacccg gtgttgttct acctcaacga acagggcgca ggccgcagcg
     1561 aaaccgccgc cagcgcgctc accctgttgc tgcgcagctt aggtattccc gcaaggctca
     1621 gctgcggctg ggccctccag cagcgcaacc ccttcaccgg cctgttcgac ctcaccgccg
     1681 ccaacgccca ctgctacagc gaggcccaca tcaacaacca cggctgggtg gaattagaac
     1741 cctccagcct gttcgccgcc cgcccctcag ccggtcgttt aagcctgggc gttatcgaat
     1801 ggcagcgaac tctcacaagc ccctggcgag cactgctcac cggttttagc acccttgcgc
     1861 tgatcgccct ttttgctgca gtcctttggc gcctgtggcc caccgtgcaa aaaagccccg
     1921 cccttatccg ctggcggctt aaacgcctag ccaagacccg ccttacctct gacgaccccg
     1981 ccgccgagtc cctcgccaac ctcatcaacg cctgcaaact gatcggttat cagttccccc
     2041 ccggcagcgg catctatccc tgggcctgcc tgtggcaaac cctactgcca agcttcgatg
     2101 ccgagcgcta ttgcgccgat ttttatcacc accacttcgg cctaaacacc caaggcgata
     2161 tctgcacgca ccaccagaca cttctcaaac acctgtgtga gttgccctgg cctgagctta
     2221 cccgctgggt aacgccgcac acccaggcag catgagcgcg cccttagcct gcgccaattt
     2281 tgctaaagta gcccgccttg atcgagcgta aagccgccag tgaactattc agccataggt
     2341 gcttcaaagc gcttgactat tgtgcctgcg cccccagata gcctggctgg cgaggccacc
     2401 actggagtgc tacactcccc agcagattta caggaggatc agtaatgggt acatacctta
     2461 aaaccctcga tatgcttgcc cccgggcaaa acctcaacgc ctatgtgcag gccgtaaatg
     2521 ggttttctgt gctgtcggcg gaggaggaaa aagccctcgc cgaagagctt tattaccatc
     2581 aaaacctaga agccgcccgc aaactggtaa tggccaacct gcgctttgtt atccacatcg
     2641 ccaaatccta tgcaggctac ggcctggccc agagcgacct ggtgcaagag ggcaatgtgg
     2701 gtttgatgaa ggcggtgaaa cgctttaacc ctgaaaaagg cgtgcgcctg atttcgtttg
     2761 cagtgcactg gatcaaagcg gaaatacacg aatttatcct gcgcaactgg cgcatcgtca
     2821 aaatcgccac caccaaagcc cagcgcaaac tgttctttaa cctgcgcggc cagaaaaaac
     2881 gcctaggctg gctcagctac gacgaagcca aggccgtcgc ccaggattta aatgtagaag
     2941 tcaaacacgt gcaggaaatg gaaggccgcc tgtttgcctc cgatgccgcc tttgatgccg
     3001 gtgttgatga agacgacgat accgcttacc aagcgccggt taactaccta gaagaccgcc
     3061 gttttgaccc tgctgccatg ctcgaagact ccgactggga aacctccagc gtgaacagct
     3121 tggaagttgc catagaacag ctcgacgagc gcagccgcga tattctgcag cgccgctggt
     3181 taaatgaaga taaggccaca ttgcatgatt tggcggctca ctatggtgtc tcggctgagc
     3241 gcattcgcca gctagagaaa aacgccatga aaaaagtgcg caccatgatg gaagcctaag
     3301 cctgcattaa caggccattt caaacctcaa tacccgctct taagcgggta tttttttgcc
     3361 caagtttttg gctgcaaatg ataagagcaa aacccaaagt tgctaaaagg catttattct
     3421 gcgattgagt gcaggcgtaa aaaacgtaat tcttctgtac tttttagtgt aactgggcgt
     3481 gatacatgcg ccgactcaga accatcgggc gagcgctttg gtgctgcgga cccgcagtga
     3541 taaatacagg ttattgcaga cacgctgtga acgcatccgt gtaggctcgt caaaaaggtc
     3601 cctctttttg acggtctgca ataacctgta ttcaccactg ccacaccgcg aatcgattgc
     3661 cgcacggcta aatccccctt aatttgccac ccgcttagga gtggttaatt aacaaggcgt
     3721 gctaaatagt aaaaaataga gggaaggacg ggcaaagaag aggatgtcat gaaaagaaaa
     3781 aagctcgccc cgcaagcggg gcgagcaagc ccaattaaat gggtttagca gtagccagat
     3841 cgtaacccac gcgctgagga ttagagtttt tattggcggt atcaaaatca ttgtaattta
     3901 cttcgatttt agaaaagcta atgctgatgc tctccaccgg gtcgccatcg ctcgaggcgc
     3961 taattgaata gccgctcaca atggtatcga acaaatcgat attcatatat tcaaccactt
     4021 tatcagtgcc ggtttgcaca aatttaaatt tggcagtttt accggcagaa cccactacag
     4081 attcacgaaa gaaatctgta accgaattat ccgccgcttt ggtaatggtg acttcactaa
     4141 tggatgggcg agtggcttca cggtttgcca aattgcctgg ctccatgctc acaccgcggc
     4201 ccacgccaaa ttgcagtgaa gaaatttgaa tatgatcttt gtaaccatct gcagttacgt
     4261 tgcctttgat gccttcgtag ataagataaa tagccatggt agttctccgc tgaaaaatta
     4321 attttgtgta gtgtgaaatt taataagtga aaatttttta tttgtcactt ggtgagagtg
     4381 atactagaca aagcctcgag cacccacaat agggtgaatt tcagcacccg cgcagccagc
     4441 ctccaattgt taaaaaagcc gtcagttagt ggccacaaac ccataaaaat ggtctcgatt
     4501 tttcagccaa tacctgcggc atgaatttag gcctttagca aatcgccaca ccacactcca
     4561 ttgacctgct gtgccttagg ggtgctaaaa aatagttttt aatggggtgc ggcaggttag
     4621 taaacggcat tttgagcgcg caaaatattt ttatcgaaca tctcaatatg tgacacgcat
     4681 cacattaacg actgacaact gagcggctag cggcagcctt gatcaacacg aggcaccttc
     4741 caccccgcgt gtacacaatc cggcacaggg cactcagtag acccagtcct gcgtaatggc
     4801 ccaatgggca aacagtatga tcattgtgaa aattaactga gcctttttgc cctgctccat
     4861 accctctttg tgctaactgt aaatgtttta acttaatacc cgccgtttgc ttaggcttcc
     4921 tgccaaccat ttgttgaaag caaattaaat aggcagcatt ttgcagccgg ctattaaccc
     4981 gacggggcaa caattaaatc tgagggctaa aacatcgcgg ggaagcttgt gacggtgcca
     5041 tagggatgtg attacgcgcg tgcacactat ttgagaattc ggacttaatc agagcgcgcc
     5101 taaatatttt ttacagcaat ataagttatt gagaagtgtg tcatatctta ttaactaacc
     5161 gtcgcttagt aaaaatcaac cagattctct actctgtgca ttcaatttac tggcggctat
     5221 accactgggc ggccagcttt ttagggctat tgtttaaacc aaaaaatacg cacagcgagc
     5281 atgctatgac gataaaaact tcttccttac atttttctgc cttatcaaag ggattattaa
     5341 aagccgttgc tttggcaaca acccttgccg ccgccaccgc gcaggctaac gtgcccgcta
     5401 tctctgtcag cggcaaacaa gttttattcg acgggaaagc tggcagcatc gcaggccata
     5461 gtttgttttg gagcaatacc ggctggggcg gcgagaagta ttacaacgct tcagtggtgt
     5521 cttatattaa aaacacctat aaatctcagc tagttcgcgt ggctgttggt gttgagggct
     5581 ccggcagctt ggtgaccggc tcagcagcaa ctgcaacggc gcctgccaca caaccggacc
     5641 cgtccaacaa aacccgcgcc tttgccgtca tcgacgccgc aattgctaac gatatgtacg
     5701 tgatcgtgga ttggcacacc cacaacgccg aaaaatacca aagccaagct atttcttttt
     5761 tccgcgaagt ggcctccaaa tacggcacca acaatcacat catttacgag gtgtacaacg
     5821 agccgctgca ggtgtcttgg agcaatgtca taaagcccta cgccgaagcc gtaattggcg
     5881 aaatccgcgc aattgacccg gacaacctaa ttattgtggg cacgccgtct tggtcgcaaa
     5941 atgtggatga agctgcgcaa aaccctatta cgcgctttaa aaacatcgcc tacaccctgc
     6001 acttttacgc tggcactcac aaatctgaac tgcgcgccaa agcgcaaacc gcacttgatc
     6061 gcggtattgc cttattcgtc accgaatggg gtgcggtgaa cgccgatggt gatggcgccg
     6121 tgaactccag tgaaaccaac acctggatga acttcctcag aagcaacaac atcagccacg
     6181 ccaactgggc gttgaacgat aaaaaagagg gcgcctcctt cttgaatgtg ggtgcaagcg
     6241 ctaccggggg ctggaatgac agcaacctaa ctgaatccgg ccgtttggtg gcaagcctaa
     6301 ttcgcggctg gcccggcgtg agtacgacta acagctcaag ctcaagtagc tcatcgtcaa
     6361 gttcgtccag ctccagcagc tcttcatcga gctccacggg gcgccccagc agctcttcgt
     6421 caagcagctc ttcaagctct agcagttcat caagctcaag ctccagcagt tcatcaagct
     6481 cctcttccag ctcctcaagc tccagctccg gtggcgacac aggcgcggtg gccggtgctc
     6541 tgggctggtt ggaaggttta agcctgctgc tattagcgcc tttagcctgg cgccgcaaag
     6601 ctcgcgctta aatttggttc aataaaaaaa acccgcctcg gcgggttttt tttatgcctg
     6661 cccctatgcg tcggcgaagg gcattacctg ggctatatca tcgcaaccgc tcaagcgcat
     6721 gagcagacga tcaaccccca gtgcaatacc tgcgcagtca ggaataccct ccgccagggc
     6781 ggcaagtagc ctgttatccg gttcgatcag cggtttatgc tgggtttgac ggctcaggtt
     6841 atccgccgca aagcgcctgg cctgctcgca ggcgtcggtc agctcccagt agccattggc
     6901 aagctcaagc cgatcccaaa aaatctcaaa ccgtttggct accttatcgc cccgccgatt
     6961 gggcatcacc ctagccagcg ccgcctggca caggggatag tcctccacca ccaccaggcc
     7021 atcggcaaga ttcggctcca ccaggtaact aaaaattaag tccaaccaaa aatgcaccgg
     7081 ctcgttgcta aagtccacct gcagccgggc gcgggcaaag gtttcaagct cttcggcaca
     7141 agccctatgg gggttgaggc ccgtacactc gctgaataag gcgccgtaat caatgcgctg
     7201 aatgggtgta ccagggctga gctcgctcaa tagagcgagg gtttcggcaa ttaaatcctc
     7261 gtccgtaaag ccctctcggt accactccag cagggtgaat tccggcctat gcctgcgccc
     7321 ctgttcatcc tggcggtagg ctttgcccag gtaaaaaata tccccgctgc ccatggccaa
     7381 caaccgcttc atgaagaatt ccggcgaggt ttgcaagtaa cggatattgc tccccacttg
     7441 gcaactaatg gcgtccaggt tggggtcggt gacggtgaat gccccaaggc tgggaacgtc
     7501 aacttcgagc acattgcgag cataaaaaaa cgcgcgcaag tgctgtaaca gctgcgcgcg
     7561 tttttttaac agggaaatgc ttgcggtaga ggcaaacaca caggcttatt tgttgacgcg
     7621 acctaggtat tcgccggtgc gggtgtccac ccgtatgact tcgcccgtgc ttaaaaacag
     7681 cggtactttt actacggcgc cggtggagag tttcgcaggc ttggtgccgc cagtggcggt
     7741 atcaccgcgc aggcctgggt cggtttcaat gatttccagc tccacgtggt tggcagcggc
     7801 aactgctaag ggtgagccgt tgtagagggt gacggtgcag ttatcttgtt ctttcagcca
     7861 tttgatggaa tcgcccaccg ctgcggcatc agcctgtacc tgctcgaagg tttccacttc
     7921 cataaagtgc caaaactcgc cgtcgttgta gaggtactgc atgtcgcggt ccaccacatc
     7981 ggcgccttct agggtatcgc cagacttaaa ggttttttct aaggtgcggc cggttttgag
     8041 gtttttcagt tttacgcggt tgaaggcttg gcctttgccg ggcttaacaa actcgttttc
     8101 aatgatggaa caggggtcgc cgtcgagcat cacttttaag ccgccgcgaa aatcgttggt
     8161 agaataacta gccattttgc cctcaactcg gtttaatcaa acatttgggt gataaaaaag
     8221 gcgcacatga taccccgaac catcgctact tggcacataa aaagctggca ggaagagtta
     8281 agccagttgg tgcgcagccc tgcagacctc ttcgcccgcc taaacctcga tccggccctg
     8341 ttgccggcgg cgaacaaggc cgcccaggcc tttcccgtgc gggcgaccga gtcctaccta
     8401 gcgcgtatca agcccggaga ccccaacgat ccgctactgc ggcagatcct gcccttggat
     8461 gcagagctct ccagccccgc cgactacagc cgcgaccccc tccacgagct tgagtccaac
     8521 ccggtgccgg gccttgtgca caagtacgca ggccgcgttt tgctggtggc cagcggcgcc
     8581 tgcgccatca actgccgcta ctgttttcgc cgccatttcc cctatgaggc caacaccccg
     8641 gggcgcgagg attggcaggc ggctctgaac tatattcgcg ccgatacttc cctagaggag
     8701 gttattctca gcggcggcga cccgttggcg gtgagcgata aacaatttgc ctggttacta
     8761 caacagctgg atgccatccc ccatgtgagc cgcctgcgta tccatagtcg tttgcctatt
     8821 gtgttgccca gccgcataag ccccgaatgg gtagatattg tgcgcagcag tcgcttggac
     8881 aaggtgctgg tgattcacgc caaccacccc caggaactcg acgccagcgt acaagcggca
     8941 ctggggcgct tagcggccat cggggtgatg ctgttgaatc agagcgtgct gctggcgggc
     9001 gtcaacgatg aagcgccggt gctcgccgat ttgagccgaa cattatttgc ctttggcgtg
     9061 ctgccctatt acctgcattt gctggacaag gtggatggtg cggcccactt tgcggtaccg
     9121 gatgaaaaag ctgcggaact caaacaggct ttgatgcaga ccttacctgg ctatttggtg
     9181 ccgcgcatgg tgcgggagat cgcaggcgcc agctcgaaaa cgccttttta attcggcggc
     9241 tatacttggt tagagtccga ggtatgattg accagatcgc ttggaacttc gtcaaggggc
     9301 gccgcaaaga cgtatcgggc gccgcagcta gagtattttc gcagtgccgc tgggaataaa
     9361 gctgtacccg tgcggctaaa aagttattct gcaggggtta gaaccgtggc gttgcctgca
     9421 cttgccggct cacacaccat aactgggtat ccgacgagcc tggtgtagat gtgcgtacgc
     9481 gcagccctaa caacaataat taattaccga tgggacgcgt taacttaatg gatgagctaa
     9541 aggtacaacc gatgacagac acttttgacc ctttattaat gcttcgctcc ctgggacttc
     9601 ccgaggcgga tgccacgcaa ttaaattttt gcggcggcgc ccggcaagcg aaggtcgccc
     9661 agtgggtaga cgacctaaaa gccacccagg tatcccaaac cggcgtggcg ctttataacg
     9721 ccctgccgga ggtgctgcgc ttaaaagcgg atccactggc taaatacgag attttagaga
     9781 ccctgcggcc tgcggtgcag cagtgtatcg ccgggctcgc cctgacgtat ttgcaccaac
     9841 cggtgaccct atcggagtcg gcgcaaaaga ccgccatagt ggcccaggcc ttggaaaaac
     9901 acttgatcga tggctactcc caggtggtgc tggagctggc ctcggcgaac aaatccaaca
     9961 actacggccg ccatgtgttt atatgcgcgg tacaccgggc gttgacctcc ctcggcctgt
    10021 tgttttgccg ctcctttcaa ctctacaccc aggtaccgcg cgggctctgg cggcacctgc
    10081 acagcctcta ccgcacagca gaggcctacg acctcgacga cctcaccgcc aacgacccaa
    10141 tcctcggcgc cccgctgcta agtgtgcgcg aggcttattt gcgggtactg gccgccggtg
    10201 tggcgcgctt aaatcaggtg gcgcccatgg aagctatgcg ggtgttccac gccctgggca
    10261 actgggcggg gcagattcgc ctggaaccca tggtggccga tgaccccaat gtggccttct
    10321 ggctcaatct cgatgccgat cgcccgcccc tagggcgcaa ccgctaccta aaaacccagg
    10381 gcggcgacgt gcgctcactg gatttcagcc gcatcctaga ccagctacag cgcctggcca
    10441 gcgccgcccc cgaggcaggg cgcagcagtg acgacctgcc catttcactg cttaaccacc
    10501 tctgccaagc ctgggaggga gaaacccacc gggatatgga tcgacgcggc accgccgtaa
    10561 tggccaaagt cgcggtgggc ctatttgaat gccactatca actggctggc gagcgcacct
    10621 tcgacgagtt tatgcaagag cctgaagaca tagaggaagt ggaagtaacc ctgccgagct
    10681 tcgccaatct cagcagtaaa aacgaccaga aggaacaggc gcacaccggc ttagggcaag
    10741 tcttcgacgt gcaaataatc aatcaaagca gcggcggcta ttgcctgcaa tggcaggcgg
    10801 acaccgaagc gcgcttagag gcgggcgatc tcatcgccat ccgcgaacag gggcggcgca
    10861 gctggaacct gtgcatggtg cgctggctgc gcaggctcaa agacggcccc caggtggggc
    10921 ttaaacgcct cgccaacaat ccccagccca tcgccctcag cgcccgcttg gacgacggcg
    10981 actactgccc ctttatgcgc ggcttttggc tgccgggcac ccaagcgccc ggcggcaaaa
    11041 cggtgctggc cccctcccta ccctttcaag cggggcacaa catccgggtg aaggtagacg
    11101 gcaatatcac caccggccgc ttagatcagt gcaccggggg cagcgcaaaa gtgcgccact
    11161 tcacggttag catgaactca gggtaatcct gactgggtta aattgtggtt ttcaatatca
    11221 agcgatcgcc caagtttgat aggatgttcg gccctgatcg cctctttccg ccttcaaact
    11281 cttctggata cctgctgcga atatgatacc cgccaccttg cgattactca ttatccacga
    11341 cagccgcagc gaagcggagc gcctcatcag cctgctgcgc aacgccgggc gccaggtgcg
    11401 cgcgcagtgg gtggaaagcg aggaggccct ggttaaactg ctgcaagaag ccagttggga
    11461 tctattagtg gtgggcgata ccgccaaggt gcttagcgcc tcagacgcca tcaaacaggt
    11521 gcgccgcctc gcccgcgatg tgccggtaat cctgctcact gaagagggct ctgccaccac
    11581 tgtcgtggac gggctcaaac tcggcgccgc cgacgtggtg tgcatggatg aggatcaaca
    11641 cctgctgctg gtgatcaacc gggaggtcac caaccgagag gagcgccagg gccgccgcta
    11701 tgccgagcgc cgcctaaaag aagtcgaacg gcgcaaccag cagctgctgg ttgcgccgtt
    11761 cgacttcttt
//
LOCUS       DAOQGS010000049        11619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2566_length_11619_cov_5.677707, whole genome shotgun sequence.
ACCESSION   DAOQGS010000049 DAOQGS010000000
VERSION     DAOQGS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2566_length_11619_cov_5.677707"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(689..1162)
                     /locus_tag="PKZ52_03090"
     CDS             complement(689..1162)
                     /locus_tag="PKZ52_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNG58784.1"
                     /translation="MKNSKNINLPSSLDSHIGFWLRLVSNQVSARFRALVETQAGCSV
                     SEWVALRVLYDQPACTHGELIDSLGMTKGATSKVISRLEAQGLVKRVLAAGSNREQLI
                     VLTTAGKKLVPRLAALADENDSHFFATLSAAEKSALMAALQKLVAPHHPPGPPID"
     gene            1422..2090
                     /locus_tag="PKZ52_03095"
     CDS             1422..2090
                     /locus_tag="PKZ52_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNG58785.1"
                     /translation="MPTLSLRLSALSLCAVLFSGCATEHSRTLDTPKPPVAASNYQGQ
                     KPLISVGKFDNRSSFMNGIFSDGVDRLGSQAKTILITHLQQSGRFNVLERDNMAEAAQ
                     EAQLMKKTQTTLGARFVLTGDVTEFGRKEVGDQQLFGILGRGKQQIAYAKVSLNLVDV
                     QTSAVVFSSQGAGEYSLSQREVVGFGGTSSYDATLNGKVLDLAIREAVSKLAQGVEQG
                     AIKP"
     gene            2304..2624
                     /locus_tag="PKZ52_03100"
     CDS             2304..2624
                     /locus_tag="PKZ52_03100"
                     /inference="COORDINATES: protein motif:HMM:NF027394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HNG58786.1"
                     /translation="MSGCASQPTLYRWGDYDAQVYSHFKNTGSLEEQIAALEKTLATG
                     KQTQNPGPGIYAHLGLLYAKQGREDLMRKSWEKEKALYPESAAYLDFLMNRKKPSLPA
                     GAKP"
     gene            2621..3265
                     /locus_tag="PKZ52_03105"
     CDS             2621..3265
                     /locus_tag="PKZ52_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HNG58787.1"
                     /translation="MKQLLTMLALMWLTGCATQAPYNYSAFKAANPRSVLILPPNNHS
                     PDVNAPSAVLAQMTRPIAEAGYYVIPVTLMEASFRENGVYSGAEAQAINIKKLHEIFA
                     ADTALYTDITEYGNTYRLISAETAVTLRAKLVDLRTGITLWEGSARASSSEQNNNSGG
                     GLAGLLIQAAVQQVVNQLADNSYTFAGIASTRLLTPSPNGLLYGPRSPKYQPGK"
     gene            complement(3305..4288)
                     /locus_tag="PKZ52_03110"
     CDS             complement(3305..4288)
                     /locus_tag="PKZ52_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNG58788.1"
                     /translation="MSPQPHNLHFKSISYQALKPGRKLIILGAVHGNEVCGTQAITRI
                     MAEIDRGDIILRQGKVTFVPITNPLAYAKNERSGDRNLNRNLFPSENPQDFEDHIANW
                     LCPLLAEHEILLDLHSFHTPGLPMVMMGPENNSGTLEAFKFAEEENALAVRLGVNRFV
                     DGWLDTYAKGVERRQQLNIHPQDRQHLLNTDVRYGVGTTEYMREQGGYALTLECGQHA
                     DPKAQDIGYQAIINTLVYLGFIDGDEPVPVAEKQSLRLYEVIDRNDPADQFIQEWKSF
                     DEIKAGAPVGRRADGTLVCAESDGFIVFPNPKAQPGQEWFYLARPTDRLSD"
     gene            complement(4300..5121)
                     /locus_tag="PKZ52_03115"
     CDS             complement(4300..5121)
                     /locus_tag="PKZ52_03115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNG58789.1"
                     /translation="MSKTAVTLIHQGASAIDLVDLIASHCALSKAAIKRGLTFGGGWI
                     KPAGQSAFKRCRKAKASIKPGDTCAFYFDEHLIAQQWPQPEVIFEHEHFGIWYKPRHQ
                     VTQGSKYGDANSLERQINQLRQKPVWLVHRLDSAACGLVLFAYTAAACAKLNNLLQNQ
                     QIHKIYQAQVLGQIAASGRIDVPLDGKNALTEFTRIAVDEHSSLVSIRLHTGRLHQIR
                     RHFAALGHPLLGDPRYGSSNAHSDGLKLVASELRFTDPISRTPLSITLPREYCLF"
     gene            complement(5125..5991)
                     /gene="murI"
                     /locus_tag="PKZ52_03120"
     CDS             complement(5125..5991)
                     /gene="murI"
                     /locus_tag="PKZ52_03120"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNG58790.1"
                     /translation="MSQSSTDVRRHHVLVFDSGLGGLSIAREIIAAEPHCHISYLADT
                     AYFPYGTKSDGELITRIEAVIEKALLLLAPDIVVIACNTASTLALSHLRQRFNLPFVG
                     VVPAIKPAAGLSATKTLGLLATPATVSRPYTAQLIADFAGDCRVIRFGSHSLVATAEA
                     SLCGVAPAPLQIEQELAGLFAQTAGGDIDTIVLACTHFPLLTHSLKQAAPHIRHWVDS
                     GAAVARQVGVWLRQVPPSAKVCGAVQFFSTQAYGQLQLPQVQTLLGREVSVDLSLLLP
                     APNAAQARHSNH"
     gene            complement(6032..6580)
                     /locus_tag="PKZ52_03125"
     CDS             complement(6032..6580)
                     /locus_tag="PKZ52_03125"
                     /inference="COORDINATES: protein motif:HMM:NF040117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HNG58791.1"
                     /translation="MPTKALTETAAATQGGLAPSEVVIGTLVALDATGRALVSFFWGD
                     QLMEQVPALTTQIIDNRSLGRQVALLFAAGNQQQPVIMGFIHSPLDVVIGQTISGSEQ
                     ASELVETELFPSPAQFTPAERSANLDGKRLVFEAEEEVQIKCGEASINLYKDGRVVIR
                     GRNLISRASAVNRILGGSVQVN"
     gene            complement(6570..8606)
                     /gene="tssI"
                     /locus_tag="PKZ52_03130"
     CDS             complement(6570..8606)
                     /gene="tssI"
                     /locus_tag="PKZ52_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNG58792.1"
                     /translation="MAKLSQDNRLMAISDFGLGKDAFLATDFTGTEFLCGLFEYEVEV
                     LSDIHDLTPDKVVGKRGTVSVQNDQKRKFNGFVRSVTMGETQGHDYRRYRFVLVPWLW
                     FLGKTQGNRIFQNKTTKDIISKVFSDLGFNDFEFRGCATAPREYCVQYGESDLNFVLR
                     LIEEEGFVYYFKHTDAKHQLIISDAFHSPEKLPETNLEYSQGSNNNAQIFSWNRSYQF
                     RKGAWTLNDYAFKTPPKDLKANSKTVSNFVDNKKYEHYEYADLGLHDQGSASSRVKKR
                     LEAEEVARDLTKASTNCSSFFAGGTFKVAKHSHATEKGEFTITEIQHFITEHSYFQEG
                     GNTSNYENHLVCMPADLPYRPALVHQKPVMRGPQSAIVVGPKGEEIYVDSFGRIKVQF
                     IWDREGKKDENSTCFVRVMQIWAGNTWGASFIPRIGHEVIISFLDGDPDKPIVTGSVY
                     NGDNKPPYESKTQSGIKTRSTKSGTGANFNELRFDDKIGSEQVYMHAEKDFDSVVENN
                     QTRQVGNDRSLTVGHDESTSIGHDRTESVGNNESITIGNNRDKTVGKNQTESIGANKT
                     ITVGGNHTESISGKVVIDVGGTHTETVTGNITISTDGNFSETVKGTYTESVTKDYSVK
                     AKTITLKADDSITFQTGSAKITMSKNGDITISGKNVTVKGSGDVVLKGSKVSAN"
     gene            complement(8641..9057)
                     /locus_tag="PKZ52_03135"
     CDS             complement(8641..9057)
                     /locus_tag="PKZ52_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58793.1"
                     /translation="MRYVAAIIVNLLCLNISAEAQTQVLCLDKIVPASAEINPAIKMA
                     GWDVLVSDMPIWLTNISVYDGPVVQNAALVPNAKKNKEEYWDFKNHPGALFLSCEYAN
                     GAFKLTKPLPAGVTACKASVKKSSSTGMLQGGFICW"
     gene            complement(9057..9506)
                     /locus_tag="PKZ52_03140"
     CDS             complement(9057..9506)
                     /locus_tag="PKZ52_03140"
                     /inference="COORDINATES: protein motif:HMM:NF033857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSL0067 family protein"
                     /protein_id="HNG58794.1"
                     /translation="MQIFEREYLSLAVWNIGNLSMTYIYSSVEDLHGMPKVGTKHCVA
                     LVQHYAKAPVTGLWKEGAVVLGTSTLKKGTVIATFVNGKYANLPQGNHAALYISQDSG
                     GIWVMDQWKDDVKKPTISKRYIRKLGQNADGSYLDPSNNAGAYRVVE"
     gene            complement(9600..10082)
                     /locus_tag="PKZ52_03145"
     CDS             complement(9600..10082)
                     /locus_tag="PKZ52_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNG58795.1"
                     /translation="MAIYLLYEGIKGNVTADGYKDHIQIASLQFGVGRGVSMEPGNLA
                     NREATRPSISEVTISKSADNSVTDFFRESVVGSAGKTAKFKFVQTGADKVVEYMNIDL
                     FDTIISGYSISAPGDGDPMESISISFSKIEVNYNDFDTANKNSNPQRVGYDLATAKPI
                     "
     gene            complement(10507..11358)
                     /locus_tag="PKZ52_03150"
     CDS             complement(10507..11358)
                     /locus_tag="PKZ52_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNG58796.1"
                     /translation="MTTDAQKLLWIGCGELAGQTLPLLGHYAITGLSRRYKPYLAQHT
                     FWQADLNAQNLSPPLGEQNFSAIVITLAPGSYSAEAYRSTYWLNTQYIVQQLGQNAKP
                     LLVFVSSTSVYAQSDGEWVDETSPAAPVSPAAQWLLQAEQVIASSGCPFCILRPSGIY
                     GPGRDYLIRQVLQGNGGSEQFTNRIHSLDLARLLAFVLERYRRGEPVPPYLLASDDLP
                     VSSQTLRQWLAQQLGVADLTPSQEPPTRGGNKRISNRLLHTLGFALRYPTYQDGYPEL
                     CQRAKTM"
     gene            11386..>11619
                     /locus_tag="PKZ52_03155"
     CDS             11386..>11619
                     /locus_tag="PKZ52_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58797.1"
                     /translation="MTLTELRYIVVLTQEQHFGRAAERCNVSQPTLSIAVKKLEDELG
                     TILFERSKTRVYPTPLGEQIVEQAQKVLEQAAAI"
BASE COUNT         2832 a         2867 c         2927 g         2993 t
ORIGIN      
        1 cctggtagga ctgcaccccc gcctgcatca gtttgccgat aacctcacca aaatgcatgc
       61 ggccttcgtt tgaggcggta aatgttgcgc gaataatgtg ttctggtgtc tgtgtcatgg
      121 ggataaccta aatatcgaag gaaaataatg tgtaactatt caataccctt attgcccgct
      181 ccgcatgggc ggcaaatttg gggggattta ttaacccagc ggcagaatca attctgcagg
      241 cggagtattg agagcactca agcactcgcc cgatagtttc cagcaattgc tgggaattgc
      301 gcccggtttc agtttaaaag ttctgaatgg atacactatt ttttagttga tccgcagctg
      361 taaatacctt aaatgagctc tcgctgataa ttcagatcct gatgcaccta ttagcctgct
      421 ataaaaagct aaggaacctc tgaaaaatta ccttactcgc cctggcggcg ttgaatttgg
      481 caaaaaaatg ctcatttact gcgtgtaaac tccgcttttt ttgccaaatt cgcctagcca
      541 gagcttcgcc gcctacattt ttcagaggtt ccctaacccg ctataaaaag ccgccgagtt
      601 aataggtttg gcggtggtag agccacagca aagccctttt gcgtttggat gctcttcaca
      661 attttgacct taaacagaaa attcttaatt aatctatcgg cgggccaggt gggtggtggg
      721 gtgcgactaa tttttgtagc gccgccatta atgctgactt ttccgccgca gataaagtgg
      781 caaaaaaatg gctgtcattt tcatcggcca gcgcagccaa gcggggcact aattttttcc
      841 cagctgtagt cagcacaatt aattgctcgc ggttactgcc ggcggctaat acacgcttaa
      901 ccaagccctg cgcctctaac cggctgatga ccttggaggt agcgcctttg gtcataccca
      961 ggctatcaat aagctcaccg tgagtgcagg cgggttgatc atagagaacc cgcagcgcca
     1021 cccactcgct aaccgagcaa cctgcctgcg tctccactaa agcgcgaaat cgcgccgaca
     1081 cctgattcga taccagccgc agccaaaaac ctatgtggct gtctaagctg ctgggtaggt
     1141 taatattttt actgttcttc atcgaagatt ataggtttcc acggaaacat aacgacatat
     1201 tagtttccgt ggaaactcgt gtcaagcctg tgacaggtgg ccggttcggt ggcgacggcc
     1261 ttcgcgaaca cccccgcggg atgcaaccgc tcaatcgcag cagaatgcca aaaaccggtt
     1321 acaattgtgc tctcatttgc ggttgcgggc ttggtcagaa ccttcacgcc gttttacact
     1381 ccatagcact ctaccctcca acctcattag aaggacacat tatgccaact ctctctttgc
     1441 gattaagcgc ccttagcctg tgcgctgtat tgttcagcgg ctgcgccacg gagcattccc
     1501 gcaccctgga tacccccaag ccgccggtgg ctgccagcaa ctaccagggg caaaagccgc
     1561 ttatttccgt cggtaaattt gataatcgct ctagttttat gaacggcata ttttccgatg
     1621 gtgttgaccg tttaggcagc caagccaaaa ccattttaat tacccacctg cagcagtcag
     1681 gccgttttaa tgtattagag cgcgacaaca tggccgaggc cgcccaagaa gcgcagttaa
     1741 tgaaaaaaac ccaaactacc ctaggcgcac gttttgtatt aaccggggat gtgactgagt
     1801 ttggccgcaa agaagtaggt gatcagcaat tatttggcat cctcggccgt ggcaaacaac
     1861 aaattgccta cgccaaagtc agcttaaatt tagtggatgt acaaacctca gcggttgttt
     1921 tctccagcca aggcgcgggt gaatacagcc taagccagcg cgaagtggtg ggatttggcg
     1981 gcacctccag ttacgatgct actcttaacg gcaaggtgtt ggatttagcc attcgcgaag
     2041 cggtgagtaa attagcccag ggtgtggagc agggcgccat aaagccctaa gcttttattt
     2101 tgtaggctga actagcctct aattaatcac tagccattaa ttagaggcta cttaactaaa
     2161 catcccgtta acctcgcccc aaggggcgtt tagttttaac cattttatta atacaaggaa
     2221 cactcaacgt gttgacacat tttataaatg tacacttggt aagagggcgc cgtttgtggg
     2281 cgcctttttt tgcgcttgtg cttttgagcg gctgtgccag ccagcccacc ctgtaccgct
     2341 ggggggatta tgatgctcag gtttatagcc attttaaaaa taccggcagc ctggaagaac
     2401 aaatcgcggc gctggaaaaa accctcgcca ccggcaagca aacccaaaac ccagggccag
     2461 gtatttatgc ccatttaggg cttttatatg ccaagcaagg ccgggaggat ttaatgcgta
     2521 aaagctggga aaaagaaaaa gctctttacc ccgaatctgc cgcttatttg gattttttaa
     2581 tgaatcgcaa aaaaccatcg ctgccagcgg gagccaagcc atgaaacaat tgttaactat
     2641 gcttgcactt atgtggttaa ccggctgcgc cacccaagcg ccctataatt attcggcgtt
     2701 taaagcggct aacccgcgct ccgtacttat tttaccgccg aataatcact ctccagatgt
     2761 gaacgccccc agtgcagtat tagcacaaat gaccaggccg attgctgaag cgggttatta
     2821 cgtaataccg gtaaccctaa tggaggcgtc ttttcgtgaa aatggtgttt atagcggtgc
     2881 tgaggcacag gccattaata tcaaaaaatt acacgaaata tttgccgccg atacggcctt
     2941 atatacggat attaccgaat acggcaacac ctaccgctta attagcgcgg aaaccgcggt
     3001 tactttgcgg gcaaaactgg tggatttgcg caccggtatc accctgtggg agggcagtgc
     3061 gcgggcctcc agcagcgagc agaataataa ttccggcggc ggtttggcgg ggttattaat
     3121 tcaggcggcg gtgcaacagg tggtgaatca gctggcggat aatagttaca cctttgccgg
     3181 cattgccagc acgcgcctat taacccccag ccccaacggt ttgttatacg gcccgcgctc
     3241 acctaaatat caaccaggta aataggtatt taaggcgcgg ctatagacgg cagtctattg
     3301 gcggttaatc gcttaaacga tctgtgggcc gcgctaaata aaaccattct tgaccgggct
     3361 gggctttagg attgggaaac acaataaaac cgtcactctc ggcacacact aatgtgccat
     3421 cggcgcgccg ccccaccggt gcgccagctt taatttcatc aaaacttttc cattcctgaa
     3481 taaattgatc cgccggatca ttgcgatcaa tcacttcata taagcgcagg gattgttttt
     3541 cggcgacggg tacaggctca tccccatcaa taaaacctaa atacaccagg gtattaataa
     3601 tcgcttggta accaatatcc tgagctttag ggtcggcgtg ttggccgcat tctaaggtga
     3661 gggcataacc gccttgctcg cgcatatatt cagtggtgcc tacaccgtag cgcacgtcgg
     3721 tatttaaaag atgctggcga tcctgcgggt gtatatttaa ttgctggcgg cgctcaacac
     3781 ctttggcata ggtgtctaac cagccgtcca caaaacgatt taccccgagg cgcacggcta
     3841 aagcattttc ttcttcggca aatttaaacg cttctaaagt gccgctgtta ttttctggcc
     3901 ccatcatcac catgggtaag cctggcgtat gaaaggagtg taaatccaac aaaatttcgt
     3961 gctcagccaa taaggggcac aaccaattgg cgatgtgatc ttcaaaatcc tgcggatttt
     4021 cgctggggaa taaattgcga tttaaattgc gatccccaga gcgctcattt ttagcatagg
     4081 ccagtgggtt ggtaatgggc acaaaggtga ctttgccctg gcgcagaata atatcgcccc
     4141 ggtcaatttc cgccatgatg cgggtaatcg cttgggtgcc gcagacttca ttgccgtgta
     4201 cggcgccgag gataatgagt ttgcggccgg gttttaatgc ttggtaactg atggatttaa
     4261 aatgcagatt gtgcggttgt ggcgacataa aaatacttcc taaaataaac aatattcacg
     4321 aggcagtgtg atactcagcg gcgtgcggct aatcggatcg gtaaaacgta attcactggc
     4381 caccaatttt agcccgtcag aatgggcatt agagctgccg tagcgggggt cccctaataa
     4441 ggggtggccg agggcggcaa aatggcggcg aatttgatgc aagcgcccgg tgtgcaggcg
     4501 aatggacacc aggcttgagt gttcatccac ggcaatgcgg gtaaattctg tcagggcgtt
     4561 ttttccatct aagggcacat caatgcggcc gctggcagca atctgcccta atacctgcgc
     4621 ctgatagatt ttatggatct gctggttttg caataaatta tttaacttgg cgcaggcagc
     4681 tgccgtgtac gcaaataata ccaggccaca ggcggcgcta tctaagcgat gcactaacca
     4741 aaccggtttt tgccgcagct gatttatttg ccgctccaga ctgttggcat cgccatactt
     4801 gctgccctgg gtgacttgat ggcggggctt ataccaaatg ccaaaatgct catgttcaaa
     4861 aatcacttca ggctgcggcc actgctgggc aattaagtgc tcatcaaaat aaaatgcaca
     4921 ggtatcgccg ggtttaatac tggctttagc tttgcggcag cgcttaaatg cgctttgccc
     4981 ggccggttta atccagccgc cgccgaaggt taacccgcgt ttgatggccg ctttcgaaag
     5041 agcgcaatgg ctggcaataa gatctactaa atcaattgcg ctggcgccct gatgaatgag
     5101 ggtaactgca gtttttgaca tattttaatg gttactgtgg cgcgcttgcg cggcattggg
     5161 ggcgggcaat aataggctta aatccacact cacctcgcgc cctaataatg tttgtacctg
     5221 cggcagttgt aattgcccat aggcttgggt gctaaaaaat tgcaccgccc cgcacacctt
     5281 ggccgagggc ggcacttgcc gcaaccaaac ccctacctgc cgcgccaccg ccgcgccaga
     5341 atccacccag tggcgaatat ggggcgccgc ttgcttaagg ctatgggtta acagggggaa
     5401 gtgcgtgcag gccaaaacaa ttgtgtcgat gtcgccgccc gccgtctgcg caaataggcc
     5461 cgctaattcc tgctcgattt gcaaaggcgc aggggcaacg ccgcacaggg aggcctcggc
     5521 ggttgccacc aggctgtggg aaccaaagcg gatgacgcga caatcaccgg caaaatcggc
     5581 aattaactgg gcagtgtaag ggcggctaac cgtggcgggg gttgccagca ggccgagggt
     5641 tttagtggca cttaagcctg cagcgggttt tatcgccggt acaaccccca caaagggcag
     5701 gttaaagcgt tggcgtaaat ggcttagggc cagggtgctc gcggtattac aggcaattac
     5761 cactatatcg ggcgccagca gaagaagggc tttttctatc accgcttcaa tgcgcgtaat
     5821 taattcccca tcacttttgg tgccgtaggg gaaataggcg gtatcggcaa gataactgat
     5881 gtggcagtgc ggttcagcgg cgataatttc ccgcgcaata cttaaaccgc cgagccccga
     5941 atcaaaaacc aggacatggt ggcggcggac atcagttgaa gactgggaca taggcctggg
     6001 caaaagccgc cattatgcag caactaccgg ctcaattcac ctgcaccgag ccgcccaaaa
     6061 tgcggtttac ggccgaggcg cggctaatta aattgcggcc gcgaattacc acccggccat
     6121 ctttatacag attaatactc gcctcgccgc atttaatttg cacttcttct tcggcttcaa
     6181 ataccaggcg ttttccgtct aaattggcgc tgcgctcagc gggggtaaac tgcgccgggg
     6241 acgggaataa ctctgtttca accagctctg aggcctgttc gcttcctgag atagtctggc
     6301 cgattaccac atccaaaggc gagtgaataa aacccataat taccggttgc tgctgattgc
     6361 ccgctgcaaa taacagcgct acctgccggc ctaaactcct gttatcgata atttgggtgg
     6421 tcagtgcagg cacctgctcc atcagttgat cgccccagaa aaaactcacc aacgcccgcc
     6481 cagtagcatc cagtgccact agggtgccaa tcaccacctc agagggggca agccctccct
     6541 gagtggccgc tgcggtttca gttagggctt tagttggcac tgaccttact cccttttaac
     6601 accacatcac cagagccttt aacggtgacg tttttaccgg aaattgtaat gtcgccattt
     6661 ttgctcatgg tgattttggc ggaaccggtt tgaaaggtaa tagagtcatc ggccttaagc
     6721 gtaatggttt tggcttttac agaataatcc ttagtgacag attctgtgta agtgcctttt
     6781 acggtctcgc tgaaattgcc atcggtgctg atcgtaatat taccggtaac ggtttccgta
     6841 tgggtgccgc ccacatcaat gacgactttg ccgctaatgg attcggtgtg gttgccgccg
     6901 acggtaatag ttttattggc accaattgat tcagtttgat tttttccaac ggttttatcg
     6961 cggttattgc cgatggtaat ggattcatta ttacctacgg attcagtgcg gtcgtggccg
     7021 atgctggtgg attcatcatg gccgacggtg aggctgcgat cgttgcccac ttggcgggtt
     7081 tggttgtttt ccaccaccga atcaaaatct ttttccgcgt gcatatacac ctgctcgctg
     7141 ccgattttgt catcaaagcg caattcatta aaattcgccc cggtgccgga cttagtggag
     7201 cgggttttaa tgccgctttg ggttttcgat tcatagggcg gtttgttatc gccgttatac
     7261 actgagcctg tgactatggg tttatccggg tcgccatcta aaaagctaat aatgacttca
     7321 tggccgatgc gcggaataaa cgatgcgccc caggtattac cggcccaaat ttgcattacg
     7381 cgcacaaaac aggtggagtt ttcgtctttt ttaccttccc tatcccaaat aaattgcacc
     7441 ttaatacggc caaaactatc aacgtaaatt tcctcacctt ttggccccac cactatggcc
     7501 gattgagggc cgcgcataac gggtttttgg tgcaccaagg cgggccgata gggtaaatcg
     7561 gcgggcatac acaccaggtg gttttcgtaa ttgctggtgt tgccgccttc ctggaaatag
     7621 gagtgttcgg taataaagtg ttgaatttcc gtaatggtga attcgccttt ttccgtggcg
     7681 tgggaatgtt tggccacttt aaaggtgcca ccggcaaaaa agctgctgca gttggtggag
     7741 gctttggtga ggtcgcgggc aacttcttcc gcctctaggc gttttttcac ccggctgctg
     7801 gcgctaccct gatcgtgcag gcctaaatct gcgtattcgt agtgctcgta ttttttgttg
     7861 tcaacaaaat tgctgacggt tttggaattg gcttttaaat ctttgggcgg cgttttaaat
     7921 gcataatcgt tgagtgtcca tgcgcctttg cgaaattgat aggagcgatt ccagctaaaa
     7981 atttgcgcat tattattgct gccctgggag tattctaagt tggtctcggg cagtttttcg
     8041 ggcgaatgga aggcgtcgct aataattaat tggtgcttgg catcggtgtg tttaaagtaa
     8101 taaacaaagc cttcttcttc gattaaacgc aacacaaaat tgagatcgct ctcaccgtat
     8161 tgcacacagt actcgcgcgg ggcagtggca cagccgcgaa attcaaaatc gttaaagcct
     8221 aaatcgctaa aaaccttgct gataatatct ttagtggttt tgttttggaa aatgcgattg
     8281 ccttgggttt tacctaaaaa ccacagccag ggcaccagca caaagcggta gcggcggtaa
     8341 tcgtggccct gggtttcccc catggtaacg ctgcgcacaa aaccgttgaa tttgcgtttt
     8401 tggtcatttt gaaccgatac ggtgccacgt ttaccgacga ctttgtccgg tgttaaatcg
     8461 tggatatcac ttaacacttc cacttcgtat tcaaataaac cgcagagaaa ttctgtgccg
     8521 gtgaaatctg tggccaaaaa ggcatctttg cccaagccaa aatcactgat ggccattaag
     8581 cggttgtctt gtgataactt tgccatatca attaccctaa attgttgggt gaatatttcc
     8641 ctaccaacag ataaaaccgc cttgcagcat ccctgtggac gatgattttt ttacgctcgc
     8701 tttacaggct gttacgcctg cgggaagcgg tttagtcagt ttaaatgccc cattggcata
     8761 ttcacagctt aaaaatagtg ccccaggatg atttttgaag tcccaatatt cttctttatt
     8821 tttctttgca ttcggcacaa gggcagcatt ttgcaccact ggaccgtcat aaacacttat
     8881 gttcgtcagc caaatgggca tgtcacttac gaggacatcc cagccagcca ttttaattgc
     8941 agggttgatt tcagctgatg cggggacaat cttatccaga cacaaaacct gggtctgagc
     9001 ttctgcgcta atattcaagc acaagaggtt aacgataatt gctgccacgt agcgcattat
     9061 tcaaccaccc ggtaagcgcc cgcattatta ctgggatcta agtagctgcc atcggcgttt
     9121 tggcccaatt tgcgaatata gcgcttagaa atggttggct ttttaacgtc gtctttccac
     9181 tgatccataa cccaaattcc gccagagtct tgggaaatat ataaagccgc atggttgcct
     9241 tgaggtaaat tcgcatattt accattcaca aacgtagcga taacggtacc ctttttgagg
     9301 gtagatgtcc ccaatacaac cgcaccttct ttccaaagcc cagtcaccgg tgccttggcg
     9361 taatgttgca ccagagcgac acaatgtttc gtgccgactt ttggcattcc atgtaaatcc
     9421 tcaaccgaag aataaatata ggtcatagat aaatttccaa tattccaaac cgccaaacta
     9481 aggtattcac gctcaaaaat ctgcatttgc gcttgtgatc cagttaaaac cattggccga
     9541 aaaaaaagcc tagaaaatct ctaggccttt ttagggcaaa gtggtttacc caccaagcat
     9601 tagattggtt tagcagtagc caaatcgtag cccacacgtt gtgggttaga gtttttgttc
     9661 gccgtgtcga agtcgttgta gttaacttcg attttggaga agctgatgct aatgctttcc
     9721 attgggtcgc cgtcaccagg agcgctgatg gagtagccgc tgataatggt atcgaacagg
     9781 tcgatattca tgtattcaac aactttgtcg gcgccagttt gtacaaactt gaacttggca
     9841 gttttacccg ctgaacccac tactgattca cggaagaagt cagttacgga gttgtcagct
     9901 gatttagaga tggttacttc actgatagaa ggacgtgtgg cttcacggtt tgccaagttg
     9961 cctggctcca tgcttacacc gcggcctaca ccaaattgca gagaagcgat ttggatgtgg
    10021 tctttgtaac cgtctgcagt aacgttgcct ttgatgcctt catataaaag ataaatagcc
    10081 atggaattac tccttcgaac atgattaatg gggtttgtgt ttatttttgc gttaaacaaa
    10141 aattaggttt tagccgctga aggctgtttt taccgaggct ttttaaccat cagcgccttg
    10201 gtgagagtga tactagcgga attcattttt cacaacaacc ggcaattttc ccgcttctaa
    10261 ggcccccata agcccctgtt aaaattatcg gcagttagcg aacgctaacc cagccgggtt
    10321 cgcagctttt ggctttgtta agcggatgcg gtaaacactg gcgccaaggg gggccaggct
    10381 cagccctctg cacagcctat ggccacaggc ggcagcaaaa tgaaaaaaaa cgtcacgaat
    10441 tgagtaagcc tgaggatatt tcacaggaaa ataaattttt atcaggtgaa gaaaatgtga
    10501 cttatgtcac attgtttttg ctctttgaca gagttctgga tagccgtcct gataggttgg
    10561 atagcgcaac gcaaacccaa gtgtgtgcag cagccggttg ctgatgcgtt tattgccgcc
    10621 tcgggttgga ggttcttggc tcggggtaag atctgcaaca cccagttgtt gggccagcca
    10681 ctgccgcagg gtttggctgc tcaccggcag gtcgtcgctg gcgagcaaat agggtggaac
    10741 tggctctccc cggcggtaac gttccagcac aaaggccagc agccgggcta aatccaggct
    10801 gtgaatgcgg ttggtgaatt gctcgctgcc gccattgccc tgcagcactt ggcggatgag
    10861 atagtcccgc cccgggccgt aaataccaga ggggcgcagg atacaaaagg ggcagccgga
    10921 gctggcaatc acctgttccg cctgcagcag ccattgggcg gcggggctta caggcgctgc
    10981 ggggcttgtt tcatccaccc actccccatc gctttgagcg tacacgctgg tgctggagac
    11041 aaacaccaac aaaggctttg cattctggcc cagctgctgc acaatgtact gggtatttaa
    11101 ccagtatgtg gagcggtaag cctctgcgct gtaggagcct ggggcgaggg taatcacaat
    11161 agcgctaaaa ttttgctcgc ccaggggcgg gctaaggttt tgggcgttta agtccgcctg
    11221 ccaaaaggtg tgctgtgcca ggtagggttt atagcggcgg ctgaggccgg tgatggcgta
    11281 gtggcccagc agcggcaggg tttgcccggc cagttcgccg cagccaatcc ataaaagttt
    11341 ttgagcatct gttgtcatgg gagattagat aatgtgggtt aacgtatgac cttaactgaa
    11401 ttgcgctaca tagtggtatt aacccaggag caacattttg gccgcgccgc tgagcgctgc
    11461 aatgtctccc agcctacttt aagcatcgcg gttaaaaagc tggaggatga actgggcact
    11521 attttgtttg agcgctctaa aacccgtgtt tacccaacgc ccttggggga gcagatcgtc
    11581 gagcaggcgc agaaggtatt ggagcaggcg gcggccatc
//
LOCUS       DAOQGS010000050        11614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2567_length_11614_cov_5.081754, whole genome shotgun sequence.
ACCESSION   DAOQGS010000050 DAOQGS010000000
VERSION     DAOQGS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2567_length_11614_cov_5.081754"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKZ52_03160"
     CDS             <1..834
                     /locus_tag="PKZ52_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNG58798.1"
                     /translation="TRGAVRSDFGQYWNQITPENEGKWGSVEGTRDQYNWGPLDNIYK
                     YARMAGIPVKAHTFVWGAQAPTWATNNSVSATDMAAEIEEWIRDYCTRYPDTAMIDVV
                     NEAVQGHQPAAYAQRAFGNNWIQKAFQLARQYCPNSILILNDYNTFQYQHQQFIDLAK
                     PLAQGGYIDAVGMQSHDVTNYPAASLKTQLDNIYNQLKVPIYISEYDIGLTNDQQQLQ
                     NFQNHMPVLWAHPAVKGITIWGYVEGSTWITGSGLMSANGQARPSLTWLMNYIKQNPK
                     Q"
     gene            1033..2718
                     /locus_tag="PKZ52_03165"
     CDS             1033..2718
                     /locus_tag="PKZ52_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HNG58799.1"
                     /translation="MSALLISKVIRGLRPISWQGLLSSVVLTSFLTACTGDNPPQTTS
                     SSSSSSSSSSSSSSSSSSSSSSSGRTSSSSSSSSSSSSSSSSSSTSTSSGIPAGSTLV
                     YAVNAGGPAVTLNGVQYQADRFSTAGTANSTADTVQGGTLYQTERYGTFKYEVPVTNS
                     TYSVKLHFAELYQTTRAARIFSVSVEGQPAVQNIDLFDMVGQDTSYDVVINNIRVSDE
                     KLTIDLTASVDNATISGFAIYSNAGGKFVEPPITDPGGGGCDVPSSIRWTSSAAPVIN
                     PRNGDASVKDPSIVYYNNKYHVFATVYNGGYKSMYTSFADFSQAATGTYQSFAPGGSS
                     TVAPQVFYFAPQNKWYIFTQWPAKYTTSSSLESGWSAPKTLWPGNDQYGGALDYWVIC
                     DSANCYLYFFKDDGKMYYVSTTIANFPNFNVSQVKVANVQGAGGQSILFEAGNVYKLK
                     GVNKYLLLVEGWGSAESKRFFRSWTSTSLDGPWVPHKTSESDPFAGLNNVTFPSGAWS
                     GQISHGEMIRAGYDEKMEIDACKMQFLYQGANLNGYTGTYDARPYKLGLLKAN"
     gene            complement(3147..4163)
                     /locus_tag="PKZ52_03170"
     CDS             complement(3147..4163)
                     /locus_tag="PKZ52_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58800.1"
                     /translation="MLALTRTIHPINCSAIGCTILVYDKNDEAVDLIKSLSADGIIVA
                     PEHEDMGLLETIAQLSLTQSDICAVFISEEFDSEGLTGIEIATRIHSTKSNIPIFMRL
                     AGNRTVNDLPPEHHQAITGYYNTSSPERLKKITDHFLYGFYFPNYLVHIFIEAGLLTL
                     NSAIKNCEIKSSVPFLIYDYTLATEFTSILPIQLPFGNGAITFSINESDALNLIEHEH
                     TALKRTQNSNDHCNQFISEITNQFWGKVRRMCQLAYGNEQHRAPVNIPIIVNHKQKYV
                     NFGNHTPQLCFRYLLIKNHLIPEAINVEFKIIFNSILRPKDFYELIPRTTNPIDDDHF
                     EAFP"
     gene            4468..4818
                     /locus_tag="PKZ52_03175"
     CDS             4468..4818
                     /locus_tag="PKZ52_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58801.1"
                     /translation="MLIDDSAVMVNEVSSFLKGHGIDSLIACNGLEGFLLLKEHKDIP
                     LAIVDINMPVMDGLTMIERVRQELPDCSTQFIMLTTEFDRVSKERGKELGVRGWIIKP
                     FRGESVIGTIKKFI"
     gene            4820..7222
                     /locus_tag="PKZ52_03180"
     CDS             4820..7222
                     /locus_tag="PKZ52_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG58802.1"
                     /translation="MAGHIGVLLHSLPVPMLLCDCEGIVLDINQRALAVLTANPVGVH
                     LDQLLDFSRSDLVGCRTFEEVAEAMRVRSAELNVISLPINRPPVSFITVSFSFLDEGE
                     ETFILVGINDVSERFSLIKAFEYQQNLLDNILSTSTDALVVFDGRGCIELFSPAAESM
                     FGRTSTEMIIDEVHTLFDAQCHQRVNTIFNHLKSMECTDEILVFEDLLPVNSLGEVFP
                     ASITFSRSKKESNPLFFMVVSDKSLFQRFINSVNDAYIKTDSAGYIIDLNNKAENLFH
                     YDRRTLISKHISFLGIKRGQSQDVVSDIAYLIDGKGEEDYFAADKRGASLVLNLTAWP
                     QEINNVRLNNLIVRDISQKKLAEKQLILSAFTDSLTGLSNRANFNRVLAEQIKEAEGT
                     GKPFFLLVIDLDKFKEVNDSFGHDYGDRLLKAASKRLLSCVRDHDLVSRMGGDEFTIV
                     LREIDTEADLLKVAQRILRTFRREFAIKEKRIFVSASVGVAAYPQDAQYAERLLKSAD
                     MAMYAAKRAGKDAFRLFTREMYVEYERHKLIERALQKAIVNNELALHFQPKISYGRQE
                     VVGFEALLRWTTPELGFVSPLEFIPIAEENGCIIDLTRWVITQAVRSMAAWAQQCPYF
                     NDRRLMISVNISVDHFKHDLYGDLQNILEREQFDPHLLEIEITEGTLLTSASEVVKTL
                     NMISALGVQISIDDFGTGYSSLQYLKNFHLDTIKIDRAFVRDIFADPHNVLIVESIIS
                     IAKRMNLLLVAEGVESYAEIQHLVALGCDVFQGYFYSKPLPASEIPLFLASFTPVSDP
                     HS"
     gene            7565..8005
                     /gene="mraZ"
                     /locus_tag="PKZ52_03185"
     CDS             7565..8005
                     /gene="mraZ"
                     /locus_tag="PKZ52_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNG58803.1"
                     /translation="MFQGSHAINMDAKGRIAVPAKCRESLASVCQGRVVLTAHTQDRC
                     VLLYPEPEWEQILPKIEALPSFNKAALRAQRLLIGYATPLELDSAGRLLIPPTLRDYA
                     GLDKNLMLVGLGKKFELWDEAAWLNTLADDLEGELPPEMLSLSL"
     gene            8002..8970
                     /gene="rsmH"
                     /locus_tag="PKZ52_03190"
     CDS             8002..8970
                     /gene="rsmH"
                     /locus_tag="PKZ52_03190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNG58804.1"
                     /translation="MSQELHCSVLLRESVDALVQDANGIYIDCTFGRGGHTREILQRL
                     GPSGRLLAFDKDPEAIAYGRQVFAGDGRLQLIHSSFAELSAVVSELNLHGAINGVLMD
                     LGVSSPQLDQPERGFSFMQDGPLDMRMDTTTGQNAAQWINSAAEAQMMQIFFDYGEER
                     FARRIARAIVARRQKKPFERTKDLADVVAAANPKWEREKHPATRVFQAIRIFINKELD
                     DLQQGLSAAFEIVKPGGRVAVISFHSLEDRIVKRFFKEGAKGEDFPKGLPIRADQINR
                     TMELVTKPIKAGSEELKNNVRARSAVLRVAEKLSPVGQEPLNKNKY"
     gene            8973..9248
                     /gene="ftsL"
                     /locus_tag="PKZ52_03195"
     CDS             8973..9248
                     /gene="ftsL"
                     /locus_tag="PKZ52_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNG58805.1"
                     /translation="MSRFLIIALLWMGCLGSALAVVYESHLARKATAGLENTRRELNA
                     LQVESGQLMLEKSSMAAYARVESVAGEQLKMVNPSNVRFIALPKDAL"
     gene            9283..10986
                     /locus_tag="PKZ52_03200"
     CDS             9283..10986
                     /locus_tag="PKZ52_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNG58806.1"
                     /translation="MIRLLAKWRLTLLCALLGIMPGAMIWHLAQLQVLPGQERGYDFL
                     QQQGQSRTLRQEKISAYRGMITDRNGELLAVSTPVQSIFVNPQKVDSKDFPRLAKALK
                     LTTVEIEKKIAANADRKFIYLARKLPPHDAEEITQLQIPGVGVEEEYERYYPAGEVAA
                     HLVGFTNIDDHGQEGMELALDSWLTGSPGTKEVIKDLKGNVVKELGLKKSAVAGKDVQ
                     LSIDLRLQYVAHKALKAQVEERGALSGSVVVLNAKTGEILAMANQPSFNPNNRTNVPL
                     NNLRNRAITDVVEPGSTIKPFTVMAALETGRYKPDTIINTSPGYLQVGSKTLKDKHNL
                     GPIDLTTIIQKSSQIGISKLALDLEPQSVRDLLHKSGFGEITGVGFPGESAGVLPSHA
                     KWQPIERATLAFGMGMSATPLQLAQAYLMIANQGRFHSVSLLKKDAKVLDDKKPEQIV
                     SPKVAAQMNEMLKTVVQPGGTAYRARLESYSFAGKTGTAHKVDATGYAENKYVAIFAG
                     MAPADNPEIVSVVVINEPTKGSYYGGDVAAPVFAKVADGSLRLLNVPPALPRQVAAAS
                     P"
     gene            11019..>11614
                     /locus_tag="PKZ52_03205"
     CDS             11019..>11614
                     /locus_tag="PKZ52_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNG58807.1"
                     /translation="MPNIDAACARLEASGLQLDSRQVVPGDVFFALAAEPLRSTHITQ
                     ALQKGARWIVVDDALPLPSLAADRLLPLPSPKANLAQWAADFYQHPSRAMTLVGITGT
                     NGKSTTAFLLAQLLEAVTNQKGGVIGTLGFGHWCATLTDTGKTTPDAIILQRILRRLV
                     DEGITQVAMEVSSHGLDQGRVAGGHFAGAVFTNLSRDHL"
BASE COUNT         2831 a         2685 c         2988 g         3110 t
ORIGIN      
        1 actcgcggcg cagtgcgctc tgatttcggt cagtactgga atcaaattac gcctgagaac
       61 gaaggtaaat ggggctctgt ggaaggcact cgcgaccaat ataattgggg gccgctcgat
      121 aacatttata agtacgctcg tatggcgggt attcctgtta aagcgcacac atttgtgtgg
      181 ggcgcgcagg caccaacttg ggcgactaat aactctgtaa gcgcaaccga tatggctgct
      241 gaaatcgaag agtggatccg cgattactgc acccgttacc ccgacaccgc tatgatcgac
      301 gtggtgaacg aagcggtgca aggacaccag cctgcagctt acgctcagag ggccttcggt
      361 aataactgga ttcaaaaggc tttccaattg gctcgccaat actgtcctaa ttcgattctg
      421 attttgaatg attacaacac tttccaatat cagcatcagc aatttatcga cctggccaaa
      481 ccacttgccc aaggtggtta tattgatgcc gtgggtatgc agtctcacga tgtgactaac
      541 taccccgctg cctcattgaa gactcaattg gataatattt acaaccaatt gaaagtgcct
      601 atttatattt ctgaatacga tattggttta actaacgacc aacaacaatt gcagaatttc
      661 caaaatcaca tgcctgtatt gtgggcgcac ccagcggtca agggtataac catttggggg
      721 tatgtagaag gcagcacttg gattaccggc tccggcttaa tgtctgccaa tggccaagcg
      781 cgtccttcgt taacctggtt gatgaattac atcaaacaga atcccaagca gtagttttca
      841 taggtaatct cagctagccc tcttagtgga taaggacatc cacctttttt ccataaattc
      901 attagtattt cgtgtttttt gataaaacga taaaaagagg tgggttgagt gtgaagccac
      961 cgaatccctc ctggcattga tgtgggttat acaatgcagg taaatattca tcaaaacatt
     1021 gagatttgta acatgagtgc acttttaatt agcaaagtga ttcgtggcct aaggcccatt
     1081 agttggcagg gcttattatc gtcagtggtg ttaacgtctt ttttaacagc ctgcaccggt
     1141 gataatccgc cacaaacaac ctcgtcaagt tcgtcttcca gttcatcgag cagctcatcg
     1201 tcttctagca gttcatcgag tagttcctcc tcgggtagaa ccagcagttc atcaagcagt
     1261 tcatcctcca gtagctcatc cagctcatct agttccacgt ccacatcaag tggcatccca
     1321 gctggctcaa ctttggttta tgccgttaat gccggtggcc ccgctgttac actcaatggt
     1381 gtgcaatacc aggcggatcg tttttctacc gctggcaccg ccaattcaac tgcggacaca
     1441 gttcaaggcg gcacccttta ccaaaccgag cgttacggca ccttcaaata cgaagtgcca
     1501 gttaccaatt ctacctacag tgtaaaactg cactttgccg agctgtatca aacaactcgc
     1561 gccgcacgta tttttagcgt gagtgtagaa gggcaaccag cggttcaaaa tattgattta
     1621 ttcgatatgg ttggccaaga cacttcctac gatgtggtga taaacaacat tcgtgtgtcc
     1681 gacgagaaac tcaccattga tcttaccgcg agcgtggata acgcaactat cagtgggttt
     1741 gcgatttatt caaatgcggg cggcaaattt gttgagccac ctatcaccga tccaggtggt
     1801 ggcggctgcg atgtgcccag ctcaattcgc tggacatctt cagccgcacc cgtgattaat
     1861 ccgcgcaacg gtgatgcgtc ggttaaagac cccagcattg tgtattacaa caataagtac
     1921 catgtatttg ccacggttta taacggcggt tacaaatcca tgtacaccag tttcgccgat
     1981 tttagtcagg cggctaccgg cacttatcaa tcatttgctc ctggtggcag ctccaccgtg
     2041 gcgccacaag tgttttattt tgccccgcaa aataaatggt acatcttcac ccagtggcca
     2101 gcgaaataca caacgtctag cagccttgaa tccggttgga gtgcacctaa aaccctgtgg
     2161 cccggcaacg accaatacgg cggcgctttg gattattggg tgatttgtga ttctgccaat
     2221 tgttatctct acttctttaa agacgatggc aagatgtatt acgtgagcac aaccattgcg
     2281 aatttcccta actttaacgt cagccaagtt aaagtggcca atgtgcaggg cgctggtggg
     2341 cagagtattt tgttcgaggc cggcaatgtt tataagctta aaggcgtgaa taaataccta
     2401 ttgttggtgg aaggctgggg ctccgctgag tcgaagcgtt tcttccgctc ttggacatcc
     2461 actagtttag acggcccatg ggtgccccat aaaacatcag aaagcgaccc atttgccggt
     2521 ttaaacaatg tgactttccc atcgggcgct tggagtggac aaattagcca cggcgaaatg
     2581 atccgcgccg gttacgatga aaaaatggaa attgatgcct gtaaaatgca gttcttgtat
     2641 caaggtgcca acctaaacgg ttataccggc acctatgatg cacgccccta caaactcggt
     2701 ttgttaaagg ctaactagtt tgcacaccac accaagcctt gcggggtttg gtgtggtttt
     2761 ccgcttgatt ccccctcttt tttccttttt tttctcattc cttgcttcgc tcaaagcccg
     2821 ccaaagcatt ttacgccaac ctgtcggcca agttttaatc caagccgaag gcattaagct
     2881 gttggtttga tagggcgcac ccttgctatc gcgagtcgag tatacatagg cccttattaa
     2941 cccgaaggca aaatagatcg tcctgatcta ttttgccttc gccgcactag acctatgaaa
     3001 atgcgcggat tttcttgttc tagtgtggct cgcggcggtt tgcacctggg gcgcggcatc
     3061 cttgccgcct ttattaaccc ggcactaatt gccgccgggt taataattac gctcttttcc
     3121 ggagtaggcg ctaaatcacc gggttgctat ggaaaagctt cgaagtggtc atcgtctata
     3181 gggttagtgg ttctcggtat cagctcataa aaatctttgg gccttaagat gctattaaaa
     3241 ataattttaa attccacatt gatggcttct ggaattaaat gatttttaat taaaagatag
     3301 cgaaaacaca gttgcggcgt atggttaccg aaattaacgt atttttgttt gtggttaaca
     3361 atgatgggga tgttaacagg agccctgtgt tgttcattgc cgtaggccag ttggcacatg
     3421 cggcgcacct tgccccaaaa ctgattggtg atctcgctga taaattgatt gcagtggtcg
     3481 tttgagtttt gggtgcgttt aagcgcggta tgttcgtgct caataaggtt cagcgcatcg
     3541 ctctcattaa tggagaaggt aatagcgcca ttgccaaagg gcagctgaat gggcaggatg
     3601 ctggtaaatt cagtggctag ggtgtaatcg taaattaaaa acggcacaga gcttttaatt
     3661 tcgcagtttt taatggcgga gttaagcgtg agtaatccgg cttcaataaa aatatgcacc
     3721 agataattgg ggaaataaaa accatataag aaatggtcgg taattttttt tagtcgctca
     3781 gggctgctgg tgttgtagta gccggtaata gcctgatggt gctccggtgg caaatcgttt
     3841 actgttcggt tgcctgccaa gcgcatgaaa ataggtatat tgcttttggt gctgtgaatc
     3901 cgcgttgcta tttcaattcc tgtcaaacct tcactatcaa attcttcgct gataaaaacc
     3961 gcgcagatat cactttgcgt gaggcttagc tgcgcaatgg tttccagcag gcccatgtct
     4021 tcatgttcgg gggccacgat gatgccgtca gccgaaagtg acttaatcaa gtccacagct
     4081 tcatcgtttt tatcgtatac caatatcgta cacccaattg cactgcagtt aatggggtga
     4141 atggttcgcg tcaatgctag catggtgcag cctttatata gctattacag tttggatgtt
     4201 gatagtgtta accatattgc cagtgtgcat tgattgatag ctttgcagtc cattgaatac
     4261 tacttcgcca gtgttggccg cccgtgtagt gcaacgggct actcaatacg cggggttgca
     4321 gcctgtgaca tgcccaattt taccagtgta gtttatatat tgaggttttc gtacaacccg
     4381 gccatcttga tctaaaataa acaagcaggc ggcgtatggg cctgttatat caattaaaag
     4441 ggtgggggta agtctatggc gcaagtcatg ttgatcgatg actcggctgt gatggtaaac
     4501 gaggtctcct cgtttctaaa aggacatggg attgactcgc tcattgcctg caacgggttg
     4561 gagggattct tgttgctcaa ggaacacaaa gacatcccgc tggctattgt tgatattaat
     4621 atgcctgtga tggacggcct caccatgatt gagcgggtgc ggcaggagct acctgattgc
     4681 agcacccaat ttattatgtt gactaccgaa ttcgaccgcg tatccaaaga gcgcggcaaa
     4741 gagctgggcg tgcggggatg gatcattaaa cctttccgtg gtgaaagtgt gatcgggacc
     4801 atcaagaagt ttatttaata tggccggtca cattggcgtc ttgctccact ccttacctgt
     4861 acccatgtta ctgtgtgatt gcgagggaat tgtccttgat ataaatcagc gcgccctagc
     4921 ggttttaacc gctaacccgg ttggagtgca tttagaccag ttattggatt ttagccgttc
     4981 cgacctggtg ggctgccgaa ccttcgagga ggttgccgag gccatgcgcg tgcgcagcgc
     5041 cgaattaaat gttattagcc tgcccatcaa tcgccctccg gtgagtttta tcaccgtgtc
     5101 tttttcattt cttgatgagg gcgaggagac gtttattctg gttggcatca atgatgtcag
     5161 tgagcggttt agccttatta aggcctttga ataccagcaa aatttactcg acaatatatt
     5221 gtccacctcc accgatgcgc tggtggtatt tgatgggcga ggctgcattg agttgtttag
     5281 cccggcggca gaatccatgt ttggccgcac ctccactgaa atgattatcg atgaagtgca
     5341 tactttattt gatgcccagt gccatcaacg cgtgaataca atatttaatc atctgaaatc
     5401 catggagtgt actgatgaga tattggtttt tgaggattta ttgccagtta attcccttgg
     5461 tgaggtgttt ccggcttcaa tcaccttttc gcgctctaag aaagaatcga accccttgtt
     5521 ttttatggtg gtgtcggata agagtctatt tcagcgcttt atcaattcag ttaatgatgc
     5581 gtatatcaaa acggattcgg ctgggtatat tatcgacctg aataataaag cggaaaattt
     5641 atttcattac gatcggcgaa cccttatcag caagcatatt agtttcttgg gtattaaacg
     5701 cggccagtcc caggatgtgg taagtgatat agcctacctg atcgatggta agggggaaga
     5761 agattatttt gccgccgata aacgcggcgc tagcctggtg ctaaatttaa ccgcgtggcc
     5821 ccaagagatt aataatgtcc gtctgaataa tttaattgtt cgcgatatta gccagaaaaa
     5881 acttgccgaa aaacagttga tcctgtccgc ttttaccgac tctttaaccg ggttgtccaa
     5941 tcgcgctaat tttaaccgcg ttttggccga gcagataaaa gaggctgagg gtaccggtaa
     6001 gccttttttc ttgctggtta tcgatctgga taaattcaaa gaggttaatg atagttttgg
     6061 ccacgactat ggtgatcgtt tattaaaagc ggcctctaaa cgcttgttgt cctgtgtgcg
     6121 cgaccacgat ttggtttcgc gcatgggcgg cgatgaattt accattgtgt tgcgtgaaat
     6181 tgataccgag gcggatttac tcaaggtggc gcagcggata ttgcgcacct ttaggcgcga
     6241 gtttgctatt aaagaaaaac gtatttttgt ttcggccagt gttggtgtag ctgcgtatcc
     6301 gcaggatgcg caatatgcag agagattact gaaatctgcc gatatggcta tgtatgccgc
     6361 taaacgcgcg ggtaaggatg cgtttcgctt atttacgcgg gagatgtacg ttgagtacga
     6421 gcgccacaaa ttaattgagc gagcgctgca gaaggcgatt gttaataacg aattggcgtt
     6481 acattttcag cctaaaatct cctatggccg ccaagaggtg gtagggtttg aagccctgtt
     6541 gcggtggaca acccccgagc ttggctttgt ctcgcccttg gaatttattc ccattgcaga
     6601 agaaaacggc tgcattattg atttgacccg ctgggttatt acccaggcgg tgcgcagtat
     6661 ggccgcatgg gcgcagcaat gcccctattt taatgaccgc aggcttatga tctcagtgaa
     6721 tatttccgtg gatcatttca agcatgattt atatggggat ttacagaata ttttagagcg
     6781 cgagcaattt gacccgcatc tgctggagat tgaaattacc gagggaacac tgctgacctc
     6841 ggcatctgaa gtggtgaaga cactcaacat gatcagcgcc ttgggagtgc agattagtat
     6901 tgatgacttt ggcaccggtt attcctcgtt gcagtattta aaaaactttc atttagacac
     6961 catcaaaatc gaccgcgcgt ttgtgcggga tatttttgcc gacccgcaca atgtattaat
     7021 cgttgaatcg atcatttcca tcgccaagcg gatgaatttg ctgctggtgg ccgaaggagt
     7081 ggaatcctat gcagagatcc aacatttggt agccttaggc tgcgatgtat ttcaaggtta
     7141 tttctattct aaacctctac ccgccagtga aattccactg tttttagcct cctttacgcc
     7201 cgtaagcgat cctcactcct aagctgttcc gcagattaac cccagctacc cgtgctgagg
     7261 ttcctatcgt tgcattaatt catcagccgc atcccagtga tttttacatg cccccgccgg
     7321 tgtgggcggt caaaaacacg tctcgcttaa accttcattg gcttccttta gccctgcctg
     7381 ccgcctctaa ccgtacaaat tgcgaccaaa gcgtgcacta acttacgcct tggcttgcca
     7441 atttccgcgg ctttctgccc gctcgccttg actttactag gttcccgcat atagtgtatc
     7501 gaagtggtaa aaagtgtttt tttgtggagg ttggtggggt aaatgagctt gttggtgggg
     7561 atgcgtgttt caaggtagtc acgcaatcaa tatggatgcg aagggccgca tcgcggtacc
     7621 ggctaaatgc cgggagtccc ttgcgtctgt atgccagggg cgcgtggtgt tgacggcaca
     7681 cactcaagat cgctgtgtac tgctctaccc ggagcccgag tgggagcaaa ttctgcccaa
     7741 gatcgaagcc ttacccagtt ttaacaaagc ggccctgcgt gcccaacgcc tgttaattgg
     7801 ttatgcaaca ccccttgaac tcgatagcgc cgggcgcttg ttaattccac ccaccctgcg
     7861 cgattacgca ggcctagata aaaatttgat gctggtgggg ttggggaaaa aatttgaatt
     7921 gtgggatgag gcggcttggc ttaacacctt ggcggatgat ttagaaggcg aattaccgcc
     7981 tgaaatgttg agtctttctt tgtgagccag gaattacatt gttcggtttt gctgcgcgaa
     8041 tcggtggatg cactggtgca ggatgccaac ggtatttaca tcgattgcac atttggccgc
     8101 ggcggccata cacgggaaat attgcagcgc ttggggccta gcggccgttt gttagccttt
     8161 gataaagacc ctgaagccat tgcatatggt aggcaggtat ttgcaggtga tgggcgtttg
     8221 cagcttattc acagcagttt tgctgagctt tctgcagtgg tgagcgaatt aaatttgcac
     8281 ggcgccatta atggtgtgtt gatggattta ggtgtttctt cgccgcaatt agaccagcca
     8341 gagcgcggtt ttagttttat gcaggatggc ccgttggata tgcgtatgga taccacaacc
     8401 ggccaaaacg ccgcccagtg gattaacagc gcagcagaag cgcagatgat gcagattttt
     8461 tttgattatg gcgaagagcg ttttgcccgc cgtatagcaa gggccattgt ggcgcggcgg
     8521 cagaaaaagc cctttgagcg caccaaggat ttagccgatg tggtggccgc cgccaaccct
     8581 aaatgggagc gggaaaaaca cccggctacg cgggtttttc aggccattcg catttttatc
     8641 aataaagaat tagacgattt gcagcagggc ttaagtgccg cctttgaaat agtgaaaccg
     8701 ggtgggcgcg tggcggtgat tagttttcac agcttggaag atcgcatcgt taagcgtttt
     8761 tttaaggaag gtgctaaagg cgaggatttt cctaaaggct tgcctattcg cgccgaccaa
     8821 attaaccgca ccatggaatt ggtgaccaag ccgattaagg ccggtagtga agagttaaaa
     8881 aataatgtgc gtgcccgcag cgccgtgttg cgggtggcag aaaaattaag cccagttggg
     8941 caggaaccct tgaataaaaa taagtattag tcatgagccg ttttttgatt attgcactgc
     9001 tgtggatggg ctgtttaggg tccgccttgg cggttgtgta tgaatcccac ttggcgcgca
     9061 aggccactgc cggtttggaa aatacccgcc gggaattaaa tgccctgcaa gtggaatccg
     9121 gccaattaat gttagagaaa agctccatgg ccgcctatgc gcgggtggaa agcgtggcgg
     9181 gcgagcaatt aaaaatggtt aaccccagca atgtgcgttt tattgctctg cctaaggatg
     9241 cgctatgaaa aagaacaact gtgttttagt taaggacact ccatgattcg tttgcttgcc
     9301 aaatggcgat tgacattatt gtgcgcgctg ctgggcatta tgcccggcgc catgatttgg
     9361 catttggccc aattgcaggt attacccggc caagagcgcg gctacgattt tctgcagcag
     9421 cagggccaat cgcgcacctt gcggcaggaa aaaatcagtg cctatcgcgg catgatcacc
     9481 gatcgcaatg gcgaattgct ggcggtgagc acgccggtgc aatcgatttt tgtcaatcct
     9541 cagaaggtgg acagcaaaga ttttccgcgc ctggctaagg ccttgaaatt aactacggtt
     9601 gaaatcgaga aaaaaattgc cgccaatgcg gaccgcaaat ttatttattt agcgcgcaaa
     9661 ctaccgcccc acgatgccga agaaattacc cagctgcaaa tacctggtgt gggggttgaa
     9721 gaggaatatg agcgttatta cccggcgggt gaggtagctg cgcatttagt aggttttacc
     9781 aatattgatg accacggcca agagggtatg gaattggcgc tggatagctg gttaaccggc
     9841 agcccaggca ctaaagaagt gattaaggat ttaaaaggca atgtggtaaa agagctgggc
     9901 ctgaaaaaat cggcggtggc cggtaaagat gtgcagctga gcattgattt gcgcttgcag
     9961 tatgttgccc ataaagcctt aaaagcccag gtggaagagc gtggcgcgct atcgggcagt
    10021 gtggtggtgt taaatgctaa aaccggtgaa atactggcca tggccaatca gccgagtttc
    10081 aaccccaata accgcaccaa tgtgccctta aataatttgc gcaaccgcgc cattaccgat
    10141 gtggtggagc ccggttccac cattaaacct tttacggtaa tggccgcgct ggaaacgggc
    10201 cgctataaac cggacacaat tattaatact agccccggtt atttgcaggt gggcagcaaa
    10261 acgctaaagg ataaacacaa tttaggccca attgatttaa ccaccattat tcaaaaatcc
    10321 agtcaaatag gtatcagtaa attagccctg gatttagagc cgcaatccgt gcgcgattta
    10381 ttgcataaaa gtggttttgg tgaaattacc ggggttggat ttcctgggga gagcgcaggt
    10441 gtgttgccca gccacgctaa atggcagccc attgagcggg ccactttggc ctttggtatg
    10501 ggtatgagtg caacgccttt gcagctggcg caagcttatt tgatgattgc caatcaaggg
    10561 cgatttcatt ctgtcagcct gttaaaaaag gatgccaaag tgctggatga taaaaaacct
    10621 gagcaaattg tctcccccaa agtggcggca cagatgaatg aaatgcttaa aaccgtggta
    10681 caacccggcg gcacagccta tcgagctcgg ttggagtcct acagctttgc cggtaaaacc
    10741 ggcacagccc acaaggtgga tgccaccggt tatgcggaga ataaatacgt ggccatattt
    10801 gcgggcatgg cacctgcgga taatcctgaa attgtctccg tagtggtcat caatgaaccc
    10861 accaagggca gctattacgg aggtgatgtg gccgcgccgg tgtttgccaa ggtggcggat
    10921 ggctcgttgc ggttactcaa cgtgcctccg gcgctaccgc gccaagtggc ggccgcaagc
    10981 ccatgacgca tacctacaca aggcccctcg gggccttttt gccaaatatc gatgccgctt
    11041 gcgcgcgcct tgaagccagc ggattgcagc tcgacagtcg ccaggttgta cctggcgatg
    11101 tgttttttgc cctcgccgca gagcctttgc gcagcaccca cataacccag gcgttgcaaa
    11161 aaggcgcccg ctggattgtg gtggatgatg cgctgccgct gcccagcctg gcggcggata
    11221 ggctgttgcc gctgccatca cccaaggcga atttggccca gtgggcggcg gatttttacc
    11281 agcacccgag ccgtgccatg acgcttgtcg gcatcactgg caccaatggc aaatccacca
    11341 cggcgttttt attggcccag ttgctcgaag ccgtcaccaa tcagaagggt ggggtgattg
    11401 gcactctagg ctttggccat tggtgtgcga cgcttaccga caccggcaag accacccctg
    11461 atgccatcat cctgcagcgt attttgcgcc gcctagtaga tgagggcatt acccaggtgg
    11521 ccatggaagt ctcctcccac ggcctggacc agggccgggt agcggggggg cacttcgcag
    11581 gtgccgtgtt taccaatctc agccgcgatc atct
//
LOCUS       DAOQGS010000051        11558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2591_length_11558_cov_4.833609, whole genome shotgun sequence.
ACCESSION   DAOQGS010000051 DAOQGS010000000
VERSION     DAOQGS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2591_length_11558_cov_4.833609"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKZ52_03210"
     CDS             complement(<1..382)
                     /locus_tag="PKZ52_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNG58808.1"
                     /translation="MRFTKIISLAALLLAAPYALAGKVAVLNIESAVLQTEAAKKAEK
                     ALQARADYAALVAKAESLKAEFENLAKEEKTKGETWPADKKQETKKKLKSLNDDYQEA
                     VKKIQGEQQKVVMGIMQEMQPKMKA"
     gene            complement(411..3062)
                     /gene="bamA"
                     /locus_tag="PKZ52_03215"
     CDS             complement(411..3062)
                     /gene="bamA"
                     /locus_tag="PKZ52_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNG58809.1"
                     /translation="MKSKTSFKCGFVILSLAAALPSFAWAESFRISDIRVEGLQRVSA
                     SPVFAAMPLQVGDTADEDSINAAISSLFATGFFSDIQVLHEGGVLIVAVKERPAISEV
                     KLDGNKAIKTEQLNKVLKDQGIAVGQIFQKDTLDGLVHELERQYVAQGRYGAKVEAKV
                     VEQPNNQIKLDIKIDESKVSGIVNIQFVGNNTFDDKTLKNLFDLKEKGWFSFLSGNNH
                     YAKEKFKGDIEKLESYYLDRGYLDFKVVSSQVALSPDKKSVFITLNLHEGDIYKVNKV
                     SLAGDPILPEESIKMVLLLAKGDTFSQNLMTTTSEYITTLLGNAGYTNAKVEGIPQRN
                     EENKTVDIDFFINPGQRVYVRRVNFKGNTRTTDEVLRREMRQMESASAIKAKIEQGKV
                     RLERLGFFKSVEVENKEVPGSADLVDVEYKVEEQPSGSINGSIGYGQGTGAIFQAGLE
                     EKNWLGTGKEVGVAVSYNKYVTQYSINYNDPYFTPDGVSRGISLYYSKRDYGYSNVAD
                     YTTNNYGLSVNFGYPISEIQRVNFGMGFDHTEVKLQGYAGQEIIRSPGEVDLTQSAFT
                     YINSSNLYKLADKDPDGDGLYEDISYVDGLVAPQNLRISEPGFIDLYGDVYNSLNVSA
                     SWLRSTLNRGILATRGNQESLTLKVGLPSTDLQYYKLEFNGQYFQPLTENLTLRFKTD
                     LGFGDGYGDMDRYPFFNNFFAGGFGSVRGFRRYTLGPQSSPSLSYARQGTTFVADEGN
                     AGLKTVGSSFVMCDDVTLTNCGFDGEVGKLATNAQDTGGTRAYGGNVLVEFGAELLFP
                     LPFIEDQRSFQTVLFVDAGNVFDTHCGELQENCSNVDLSRLSSSYGLGLSWLSGFGPL
                     TFSISRPIHKNETDRTEVFQFSLGAGF"
     gene            complement(3164..4522)
                     /gene="rseP"
                     /locus_tag="PKZ52_03220"
     CDS             complement(3164..4522)
                     /gene="rseP"
                     /locus_tag="PKZ52_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNG58810.1"
                     /translation="MALFDFLYTLSMFVLVLIVLVGVHEYGHFQVARLCGVRVLRFSI
                     GMGKPFFSFYDKQGTEFCLAPIPLGGYVKMLDEAEGPVAASERHLSYSQKSPWARIAI
                     LAAGPMANLLLAMVVFAFLGIRGIQSLAPEIFEVKPQSVAAAAGLKVGEEIIAVDGVP
                     TTTQEAVFEQLLRRLGESGTLSLTARTTDNSEHTYTIPLRDWLQGTHEPDPFEDLGFE
                     LYRRQILPVVGQVVAHGAAERAGLKAEDKLISLDGTAIESWQQWVSLIKQSANKPLTV
                     RIERDGLLQDLVVTPDGEQQQGQVIGRIGVYPKLAPIPERLKRLRTFSVGEAIVYGFE
                     KSFSQASLVLLSIKKLVVGEISTKNLSGPIGIAKVAGDSARAGFIYYLGFLAQLSVYL
                     AVLNLLPIPVLDGGHIVYCLLEAAKGSPVSEKLKLYSAQIGLALLGCVMIVAFYNDIL
                     RL"
     gene            complement(4529..5737)
                     /gene="ispC"
                     /locus_tag="PKZ52_03225"
     CDS             complement(4529..5737)
                     /gene="ispC"
                     /locus_tag="PKZ52_03225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNG58811.1"
                     /translation="MTLLDDTLGRPQRVTVLGSTGSIGVNTLDVLSQHPERFEVFALT
                     AHQQIDKLIEQCLRFTPRVAVVPSADAAQKLQAALRQADCPTQVLQGEAGLCAVASDA
                     AVDTVVAAIVGAAGLRPTMAAVNGGKRLLLANKEALVMAGGLFMQAAERSGAQVLPVD
                     SEHNAIFQCLPYPLNSLQQAGVKRIVLTGSGGPFRLLPLEQLPFVSPEQACKHPNWAM
                     GRKISVDSASMMNKGLEFIEACWLFSARPSEIDVLLHPQSIIHSMVEYQDGSVLAQMG
                     QPDMRTPIAHCLAWPQRIASGVAGLDFLSLGALEFAAPCEQRYPALSLAKAAMGAGGL
                     APAALNAANEIAVEAFLGGQINFVQIAQLVDAVMQSWRNSEPGALEEVYAADLSARDA
                     ALRHLSKIRQ"
     gene            complement(5734..6537)
                     /locus_tag="PKZ52_03230"
     CDS             complement(5734..6537)
                     /locus_tag="PKZ52_03230"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNG58812.1"
                     /translation="MLKQRVVTALVLSAVLLGVLLAAPIGVFNACMALVCALAAWEWA
                     ALALLPAWARGLFVGAILAAAWFIFTHAPQSLALAWSCALGVWVLALFWVSRYPRLTN
                     LWANKPVRSLIGLGALLPTLLAFWYLRQQEQGIGWVVLLLCVVATADSGAYFTGRAFG
                     KHKLAPSVSPGKSWEGVLGGWAGALLLAAGLSLFSPSLSFLTCLILVMPAAMISVLGD
                     LLESMLKREAGVKDSGSLLPGHGGFLDRIDGLTAAAPVFALSYVLVLGS"
     gene            complement(6530..7273)
                     /gene="uppS"
                     /locus_tag="PKZ52_03235"
     CDS             complement(6530..7273)
                     /gene="uppS"
                     /locus_tag="PKZ52_03235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNG58813.1"
                     /translation="MSREGLNHIAIIMDGNNRWAKREGRQGIAGHKAGVERIRDVLAA
                     ARKRGVSTLTLFAFSSENWRRPPVEVDALMGLFFTYLKKEARELKDEGVNLRVIGNRA
                     RFNKTLLGAIEEAEAIASGGQDTLVIAADYGGRWDIAQAANKAIAAMGWGEIPNSDLS
                     EEILDRYMSTAALPPLDLLIRTGGEFRISNFLLWQAAYAELYFSPLLWPEFDGAALNA
                     AVDDFHQRQRRYGYTSEQIEAMSKAGPSA"
     gene            complement(7435..7992)
                     /gene="frr"
                     /locus_tag="PKZ52_03240"
     CDS             complement(7435..7992)
                     /gene="frr"
                     /locus_tag="PKZ52_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNG58814.1"
                     /translation="MISEIKKDAAERMKKTVEGLTVTFNKIRTGRAHPSLLDTVYVSY
                     YGSEMPLSQVANISVLDARTLSISPWEKAMVPAIEKAILKSDLGLNPSTMGELIRLPM
                     PALTQETRKGYIKQAKGEAEAAKVAIRNIRRDANGSFKDLLKDKAISEDDERRAEEEI
                     QKLTDKYVAEVDATLAVKEKELMEI"
     gene            complement(7996..8724)
                     /gene="pyrH"
                     /locus_tag="PKZ52_03245"
     CDS             complement(7996..8724)
                     /gene="pyrH"
                     /locus_tag="PKZ52_03245"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNG58815.1"
                     /translation="MATMRDRKYKRILLKLSGEQLMGDEGFGIDPKVLNKMALEIGQL
                     VGIGVQVGLVVGGGNLFRGAALNKAGLDRVTGDHMGMLATVMNSLALRDALERSNIAC
                     TVMSAIPMSGVVEHYDRRRAIRLLENGEVVIFSAGTGNPFFTTDSAACLRGIEVEAEI
                     VLKATKVDGVYTANPMKDPTATRYERLSYDQALAERLGVMDLTAICLCQEHKMPVRVF
                     KMDKVGALLNIVVGQDEGTLIEEA"
     gene            complement(8867..9739)
                     /gene="tsf"
                     /locus_tag="PKZ52_03250"
     CDS             complement(8867..9739)
                     /gene="tsf"
                     /locus_tag="PKZ52_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNG58816.1"
                     /translation="MSISTNVVKELRERTGLGMMECKKALEASGGDIELAIENLRKVS
                     GLKAAKKAGRTAAEGIVAIKVAADNSSGLVLEVNSETDFVARDENFLNFANSVLTKAF
                     EGKQTDIAALMDGDLEAARQALVQKVGENISVRRATIVGSAGAVVGAYVHSNNRIAVL
                     VELAGGSAELAKDVAMHVAASSPQVVNPDDMAAEIIEKEKEIIRAQPDMAGKPAEIVE
                     KMMGGRISKFLKEVSLVEQPFVKDPEVTVGNLVKKAGAKVVRFVRFEVGEGIEKETTD
                     FAAEVAAQVQASKG"
     gene            complement(9875..10603)
                     /gene="rpsB"
                     /locus_tag="PKZ52_03255"
     CDS             complement(9875..10603)
                     /gene="rpsB"
                     /locus_tag="PKZ52_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNG58817.1"
                     /translation="MPTVSMREMLQAGVHFGHQTRYWNPKMGKFIFGARSKIHIINLE
                     QTVPAFNSALETVKQMAAQKKKILFVGTKRAAQKTIAEQARRAGMPFVSQRWLGGMLT
                     NYKTIRASIRRLRDLETQSKDGTFEKLTKKEALMRTRDMQKLESSIGGIKDMGGLPDA
                     LFVIDVDHERIAVQEANKLGIKVIGIVDTNSNPDGIDIIIPGNDDAIRAIKLYVAAMA
                     DACIEGSAGKNEFVEVTDESADQA"
     gene            10779..11210
                     /locus_tag="PKZ52_03260"
     CDS             10779..11210
                     /locus_tag="PKZ52_03260"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNG58818.1"
                     /translation="MEYLYLALLSFAAGSILPLPSEALLIPLLHSSQNPWLLVATASI
                     ANTLGALTNTLLGRYLRHLAHRPWFYFSPAQMQRAQDYFRRFGLPCLLFTWLPLLGDL
                     FALAAGLLRAPWLISIVLISLGKSLRYCSVAYLVLQTQGEG"
     gene            11266..>11558
                     /locus_tag="PKZ52_03265"
     CDS             11266..>11558
                     /locus_tag="PKZ52_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNG58819.1"
                     /translation="MSQSTPTDRQVLSVSQLNGQVRQLLEALPLLWVEGEISNLSRPS
                     SGHWYFTLKDANAQIRCAMFKNRNLAVRCAVKDGDKVLLRAKVSLYEGRGDYQ"
BASE COUNT         2688 a         3249 c         2895 g         2726 t
ORIGIN      
        1 ccgccttcat tttaggttgc atctcttgca tgatgcccat cacgactttt tgttgctcgc
       61 cttgaatttt cttcacggct tcttggtagt catcattgag ggactttaat ttctttttgg
      121 tctcttgttt tttatccgca ggccaggttt cgcctttggt tttttcttct ttagccagat
      181 tttcaaattc cgcttttaaa gattccgctt tggcaaccaa ggctgcgtaa tcggcgcgcg
      241 cttgcagcgc tttctcagct tttttggccg cttccgtttg caacactgcg ctttcaatat
      301 tgagcacagc caccttgccc gccagggcat aaggggcagc caacaataaa gcagccaaag
      361 aaataatttt tgtaaaacgc ataaaaaact cccaaaaaat aaataaaaaa ttaaaaaccc
      421 gcgcccaagg agaactggaa cacctcggtg cgatctgttt cgtttttgtg gataggcctt
      481 gagatactaa atgtcaaagg gccgaaaccg gacaaccaac taagccccaa gccgtaagac
      541 gaactcaaac gacttaaatc tacgttggag caattttctt gcaactcacc gcaatgggta
      601 tcaaacacgt taccggcatc gacgaataaa acggtttgaa atgagcgctg gtcttcgata
      661 aagggcaagg ggaataataa ctcagcgcca aattccacta acacgttgcc accataggcg
      721 cgcgtaccac ctgtgtcttg ggcgttcgtg gcgagcttgc caacttcgcc gtcaaatcca
      781 caattggtta gggtgacatc gtcacacata acaaacgaag agcccacagt cttcagcccc
      841 gcattgcctt catcggcaac aaaggttgtg ccctggcgag cataactgag acttggcgag
      901 gactgcggcc ccagggtgta gcgacgaaag ccgcgcacag aaccaaaacc accggcaaag
      961 aaattattaa aaaacggata gcggtccata tcgccgtagc catccccaaa ccctaaatca
     1021 gtcttaaatc gcagcgtcag gttttctgtc aaaggctgga agtattggcc attaaactcc
     1081 aatttatagt actgcagatc cgtcgagggt aagccgacct ttagagttag ggactcttgg
     1141 ttgcctcgcg tggctaaaat gccgcggttt aatgtgctgc gcagccaact ggcgctgaca
     1201 tttaaagaat tgtatacatc gccgtacaag tcgataaaac caggctctga aatgcgcaaa
     1261 ttctgcggcg ccaccaagcc atccacgtaa gaaatgtcct catataaacc atctccatca
     1321 ggatccttat ccgcaagttt gtataaatta ctgctattaa tataagtgaa ggcagattga
     1381 gttaagtcca cctcgccggg tgaacgaatt atttcctgcc cggcatagcc ctgcaatttc
     1441 acttcggtgt ggtcaaagcc cataccgaaa ttaacccgtt gaatttcgga aatggggtaa
     1501 ccgaagttga cgcttaagcc gtaattattg gtggtgtaat cggcgacgtt ggagtagccg
     1561 taatcgcgct tgctgtaata cagggaaata ccgcggctga caccatcggg cgtgaaatag
     1621 gggtcgttgt aattaatact gtactgcgtc acgtacttgt tgtagctgac cgcaacaccc
     1681 acttctttgc ctgtacctaa ccagtttttc tcttctaaac cggcttggaa aatagcgccc
     1741 gtgccctgcc cataaccgat actgccgtta atagagccgg agggttgttc ttcaacttta
     1801 tattcaacat ctaccaagtc ggcagagcct ggcacttctt tattttccac ctctaccgat
     1861 ttaaaaaacc ccaaccgctc taaacgcacc ttgccctgtt caatttttgc tttaatggct
     1921 gaggcagatt ccatttggcg catttctcgc cgcaatactt catcggtggt gcgggtgtta
     1981 cctttaaaat taacgcggcg cacatatacg cgctgccctg ggttaataaa aaaatcgata
     2041 tcgacggttt tattttcttc gttgcgctgc ggaatgcctt caactttggc gttggtataa
     2101 cctgcattac ccagtaaagt ggttatgtat tctgaggttg tggtcattaa attctgcgaa
     2161 aaggtatcgc ccttggccaa caacagaacc attttgatgg actcttccgg caggatcggg
     2221 tcgcctgcga gggaaacctt gttgacctta taaatatcgc cttcgtgaag attaagggta
     2281 ataaaaacgc tttttttgtc cgggcttagg gccacctgcg acgagaccac tttgaaatcc
     2341 aggtagccgc gatcgaggta ataagattct aacttttcaa tatcgccttt aaacttttct
     2401 ttggcgtagt gattattgcc acttaaaaag gaaaaccagc ctttttcttt taagtcaaaa
     2461 aggtttttta gggttttatc gtcgaaggtg ttattgccga caaactgaat attaacaatg
     2521 ccggaaactt tgctctcgtc gattttaata tccaatttga tttggttgtt cggctgttca
     2581 acaaccttag cctccacctt ggcgccgtag cgaccctggg ctacatactg gcgctctaat
     2641 tcgtgcacca acccatccag ggtatctttt tgaaagattt gccctacggc aatcccttga
     2701 tctttgagca ctttatttaa ttgttcagtt ttaatggctt tgttgccatc gagcttcact
     2761 tcgctgatgg ccgggcgctc tttaaccgca acaattagca caccaccttc atgtaacacc
     2821 tgaatatccg agaaaaatcc agtggcaaat aacgagctaa tcgctgcatt aatggagtct
     2881 tcatcggcgg tatcccccac ttgcaacggc atggcggcga acaccgggct tgcggacacg
     2941 cgctgcagtc cctcaacgcg aatatcgcta atccgaaaac tttcggccca ggcaaaagaa
     3001 ggaagtgcag cggccaagga caaaatgacg aagccgcatt taaaggaggt tttggatttc
     3061 acagaggtaa aaatctttgt taatgttttt gtttgacagc cccgttatcg ggcggctgat
     3121 gtgcgttggc gcggataatt aatgaggatt taacgcatgg gagttacagc cgcaggatgt
     3181 cgttataaaa cgcgactatc atcacgcagc ccaatagagc cagtccaatt tgagcactgt
     3241 agagcttaag cttttcggat accggtgacc cctttgccgc ttccagcagg caatacacta
     3301 tgtgcccccc atctaacacg gggatgggca atagattcag cacagccaaa tacacactca
     3361 attgcgccaa aaagccgaga taatagataa atccggcccg ggcggaatcg cccgccactt
     3421 tagcaatgcc tatcgggccg ctcaagtttt tcgtggatat ttcccccacg acgagcttct
     3481 tgatggatag cagcaccagg cttgcctggc tgaagctttt ttcaaagcca tacacaatcg
     3541 cctcgcccac cgaaaaggtt cgcaggcgtt ttaagcgctc ggggataggg gcaagttttg
     3601 gatagacgcc gatgcggccg atcacctggc cttgctgttg ttcgccatcc ggggtgacta
     3661 ccaaatcctg tagcaaccca tcgcgctcaa tacggactgt tagaggctta ttggcgctct
     3721 gtttgataag gctcacccac tgctgccagg actcaatggc ggtgccatct aagctgatca
     3781 gcttatcttc ggccttaagg cctgcccgct cggcggcgcc atgggcgaca acctgaccga
     3841 ccacaggcag aatctgccgc cggtacagct caaagcctaa gtcctcgaag ggatccggct
     3901 catgggtgcc ctgcagccaa tcgcgcaggg gaatggtgta ggtgtgttcg ctattgtctg
     3961 tggtgcgcgc ggtgaggctc agagtcccgc tctcacctaa gcgccgcaac aactgctcaa
     4021 acaccgcctc ttgcgtagtg gttggcacgc cgtcaacggc gatgatttcc tcaccgacct
     4081 taaggccagc tgcagctgcc accgactggg gttttacctc gaagatctcc ggggcgaggg
     4141 actggatgcc gcgaatgcct aaaaacgcga acacaaccat cgccagcaac agattcgcca
     4201 tggggccagc agccaggatt gcgatgcgcg cccagggact tttttggctg tagcttaaat
     4261 gccgctcgct cgcggccacc gggccttcgg cctcgtccaa cattttgaca tagccgccga
     4321 ggggaatagg tgccaggcaa aactccgtgc cctgtttatc gtagaagctg aaaaagggct
     4381 tgcccatgcc gatggaaaaa cgcaacaccc gcaccccgca caggcgcgcc acttgaaagt
     4441 gaccgtattc atgcacgccc accagcacaa tcagcactag gacgaacatg ctcagggtgt
     4501 agagaaaatc gaatagcgcc atcaagggtt actgccttat cttggataga tgccgaaggg
     4561 ccgcgtcgcg ggcgctcagg tcggcggcat agacttcctc tagggcgccg ggttcactat
     4621 tgcgccagga ttgcatcacg gcatccacta actgggcgat ttgtacaaaa ttgatttggc
     4681 cgcccaaaaa ggcttctacg gcaatttcat tggcagcatt gagcgctgca ggcgccaaac
     4741 cacctgcgcc catggccgcc ttagcaaggc tgagggccgg gtagcgctgc tcgcaggggg
     4801 cggcaaattc cagggcgccg aggctgagaa aatccagccc ggcaacacca gacgctatgc
     4861 gctggggcca ggccaaacaa tgggcgatgg gtgtgcgcat atccggctgc cccatctgcg
     4921 ccagcaccga gccgtcctgg tactccacca tggagtggat aatgctttgc gggtgcagca
     4981 gcacatcgat ttcactgggg cgggcgctga acagccaaca ggcttcaata aactccaggc
     5041 ccttgttcat catgctggcc gaatccacgg aaattttgcg ccccatcgcc cagttggggt
     5101 gtttgcaggc ctgctcgggg ctgacaaagg gcaactgctc tagtggcaag aggcgaaacg
     5161 gcccgcctga gccggtgagc acaatgcgct ttactccggc ttgctgaaga ctattgaggg
     5221 ggtacggcag gcactgaaag atggcgttgt gctcggagtc caccggcagc acctgcgcac
     5281 cagagcgctc agccgcctgc ataaagagcc ctccggccat gacaagggct tctttgttcg
     5341 ccaacagcag ccgtttgccc ccatttaccg ccgccatggt gggcctaagc ccggcggcgc
     5401 ccactatggc ggccaccact gtatccacgg cggcatctga tgccaccgca cacagccccg
     5461 cctctccctg caacacctgg gtggggcagt cggcttgacg caacgccgcc tgcagcttct
     5521 gggccgcatc agcgctcggc accaccgcca cacggggcgt aaagcgcagg cactgctcaa
     5581 tcaatttgtc gatttgctga tgggcggtga gtgcgaagac ttcaaagcgc tcaggatgct
     5641 ggctgagcac atccagggta ttcaccccga tggagccggt ggagcccagc actgtcaccc
     5701 gctgcgggcg ccctaaggtg tcatctagta gcgtcacgag ccgagcacca acacataact
     5761 taaggcaaat accggcgccg cagcggtaag gccgtcgatg cgatctaaaa agccgccgtg
     5821 ccccggcagc agagaaccgg aatccttaac ccccgcctcc cgctttaaca tgctttccag
     5881 caaatcaccc agcaccgaaa tcatcgccgc aggcatcacc agaattaggc aggtaagaaa
     5941 actcagcgag gggctaaaca gggataagcc tgccgccagt aacagcgccc ccgcccagcc
     6001 gcccagcaca ccctcccagg atttacccgg cgacacgctg ggggcgagct tatgtttgcc
     6061 aaaggcccgg ccggtaaaat aggcgccgct atcggcggtg gccaccacgc acagcaacaa
     6121 gaccacccaa cctatgccct gctcctgctg gcgcagatac caaaaggcca acagggtggg
     6181 caagagtgcg cccaggccaa ttaagctgcg cacgggtttg ttggcccaca ggttggtgag
     6241 gcgcggatat cggctcaccc aaaataaggc cagcacccac acccccaggg cacagctcca
     6301 cgccagggca aggctttgcg gggcgtgggt aaaaataaac caggcggcgg caagaatagc
     6361 ccctacaaac agcccccgcg cccaggcggg caacagggct aaggcggccc actcccaggc
     6421 ggcgagggca cacaccagcg ccatacaggc attaaaaact ccaatggggg ccgcgagcaa
     6481 aacccccaaa agcacggcgc tgagcaccaa agccgtcacc actcgctgct taagcactgg
     6541 ggcctgcctt actcatggct tcgatttgct ctgaggtata gccatagcgg cgctggcgct
     6601 gatgaaaatc atcaacggcg gcatttaagg cggcgccgtc aaactcaggc cacaacagcg
     6661 gggaaaaata caactccgca taagctgcct gccagagtaa aaaattgctg atgcggaact
     6721 cgccgccggt gcgaataagc agatccaaag gcggcagggc ggcggtggac atgtaccgat
     6781 cgagtatctc ctcgctcaga tcgctattgg gaatctcccc ccagcccata gctgcgatgg
     6841 ccttgttagc ggcctgggct atatcccaac ggccgccgta atcggcggcg atgaccaggg
     6901 tgtcttggcc gccggaggcg atggcttcag cctcttcgat agcgcccaaa agggttttgt
     6961 taaagcgcgc ccgattgccg atcacccgca aattcacgcc ctcgtcctta agctcgcggg
     7021 cctctttttt cagataggta aaaaatagcc ccatcagggc gtccacttcc accggcggac
     7081 ggcgccagtt ttccgagcta aaggcaaata gcgtcagggt gcttacgccc cgcttacgag
     7141 cggccgccag cacatcgcgg atacgctcaa cccccgcttt atgaccggcg ataccctgcc
     7201 gcccttcgcg cttggcccag cggttattgc catccatgat gatggcaata tgatttaacc
     7261 cttcacgact caatgattac ctgccttctg gtacttttta accccgcgca acggcaaaaa
     7321 ccttcgcccg ctggcgcttg agcgtcactc caactatggg cacgcgcacg gtgcggccct
     7381 aaaaaacaaa aagccttttg ccgccagtag ctcggcaaaa ggcttggggc ctaattaaat
     7441 ttccattaat tctttttctt tcaccgccag ggttgcgtcc acttccgcta catatttatc
     7501 ggtgagtttt tgaatttctt cctcggcgcg gcgctcatcg tcttcgctga tggctttatc
     7561 ttttaataga tccttaaagc tgccgttggc atcgcggcga atattgcgga tggccacctt
     7621 ggccgcctcc gcttcgccct tggcctgttt gatataaccc ttgcgggttt cttgggtcag
     7681 cgctggcatg ggcaggcgga ttaattcgcc catggtggat gggttaaggc ccaaatccga
     7741 ttttaaaata gctttttcaa tggccggcac catggctttt tcccaggggc taatggagag
     7801 cgtgcgcgca tccaataccg aaatattcgc cacttgggat aagggcattt cgctgccgta
     7861 ataggagaca taaaccgtat cgagcaggct gggatgagcg cggccggtgc ggattttatt
     7921 gaaggtgaca gttaagcctt cgacggtttt tttcatgcgc tcggcggcgt cttttttaat
     7981 ttcactaatc atacattagg cctcttcaat taatgtacct tcatcctgcc ccaccactat
     8041 attcagcaga gcaccgactt tatccatctt aaaaacccgc accggcattt tatgttcttg
     8101 gcacaaacaa atagcggtta aatccatcac ccctaagcgc tcggctaggg cttgatcata
     8161 actgagacgc tcgtagcggg tcgccgttgg gtctttcatc gggttggcgg tatacacgcc
     8221 atccacctta gtggccttca agacaatttc cgcttccact tcgatgccgc gcaagcaggc
     8281 ggcagaatcg gtggtaaaaa atggattgcc ggtaccggcg gagaaaataa ccacttcgcc
     8341 attttccagc aagcgaatag cgcggcggcg atcgtagtgc tccaccacac cgctcatggg
     8401 aatggccgac attacagtac aggcaatatt cgagcgctcc aaggcatcgc gcagggcgag
     8461 ggaattcatt accgtagcca gcatccccat gtgatcgccg gtaacgcgat caaggccggc
     8521 tttgttcagc gccgcgccgc gaaataaatt gccgccgccc accactaaac ccacctgtac
     8581 acctatgccc accagctggc caatttccaa agccatttta ttgagcactt tcgggtcaat
     8641 accgaaccct tcatcaccca ttaattgctc gccgctgagt tttaacaaaa tacgtttgta
     8701 tttgcgatca cgcatagtgg ccatggaaaa ttccccttgt tgtgtcgaac attttgcgca
     8761 aaatgccgca gcggccagca gcttgcgcaa aatgtttaac ccttaaaacc ttaataataa
     8821 aaaggcggga acaacccgcc ttttttagtt ggctctcaat ggttgattaa cccttggaag
     8881 cctgaacttg cgctgccact tcggcggcga agtcggtggt ttctttttca atgccctcac
     8941 ccacttcaaa acgcacgaaa cgcacaacct tggcaccggc ttttttcacc aggttgccca
     9001 cggtgacttc tggatcttta acgaagggct gttcaaccag gctgacttct tttaagaatt
     9061 tgctgatgcg accgcccatc attttttcaa cgatttcagc aggcttaccg gccatatccg
     9121 gttgtgcgcg gataatttct ttttcttttt caattatctc agcggccata tcatctggat
     9181 taaccacttg cgggctagac gcggctacgt gcatagcaac atctttggcc aattcggcgc
     9241 tgccgccagc caattccacc agtacagcaa tgcggttatt gctgtgtaca taggcgccta
     9301 ctacggctcc tgcgctgcca acaatagtgg cgcggcgcac ggaaatattc tcacccactt
     9361 tttgcaccaa ggcttggcga gcggcttcta aatcaccatc cattaaggct gcgatatcag
     9421 tttgtttgcc ttcaaaggct ttagttaaaa cgctgttggc aaaattcagg aagttttcat
     9481 cgcgagctac gaagtcagtt tcgctgttta cttccaacac caaaccgctg ctgttatcgg
     9541 cggcgacttt aatagccaca atgccttcag ctgcagtgcg gccagctttt ttcgcggctt
     9601 ttaatccgct aactttacgc aggttttcga tagctaattc aatatcgcca ccgctggctt
     9661 ctaaagcctt tttacattcc atcatgccaa ggccggtgcg ctcacgcagc tctttaacca
     9721 cattggttga aatactcata atttctttcc tctaataaat cgcttaattg atcgccgtgc
     9781 cttttctaaa aggctttagt cgtaacaaac tactcgtaac aaaaaggggg catgccccct
     9841 ttttcgtact gcctatttgc caagtgcggg cggattaggc ttgatcggct gattcgtcag
     9901 tgacttcaac aaattcattc ttaccggcag aaccctcgat gcaggcatcg gccatggccg
     9961 ctacgtacaa tttgatggcg cgaatggcgt cgtcgttgcc ggggataatg atgtcgatgc
    10021 cgtctgggtt gctgttggta tccacgatac caattacttt aatacccagt ttgttggctt
    10081 cttgcacagc gatgcgctcg tgatcaacgt cgataacgaa caaagcatcg ggcaggccgc
    10141 ccatatcttt aataccaccg atggatgact ccagcttctg catgtcgcgg gtgcgcatta
    10201 aagcttcttt tttggtcagt ttttcgaagg tgccgtcttt gctttgagtt tctaaatcgc
    10261 gcaggcggcg gatagaggcg cggatggttt tgtagttggt gagcatgcca cctaaccagc
    10321 gctggctaac aaacggcata ccggcgcggc gggcttgttc tgcgatggtt ttttgtgcgg
    10381 cgcgcttggt gccgacaaag aggatttttt tcttctgcgc ggccatttgt ttaacagtct
    10441 ctaaggcgct gttaaaggcg ggcacagttt gctcaaggtt gatgatgtgg attttgctgc
    10501 gcgcaccgaa gatgaattta cccatctttg ggttccagta gcgagtttgg tgaccgaagt
    10561 gaacgcccgc ttggagcatc tcacgcatag aaacggttgg catgataaaa gtaccttaaa
    10621 tgtattgggt taagcctcca cagaccccac cgttcaaccc gcaaggggca cccagaacgc
    10681 gtgtgacgga atgtgtgtga cataatggat gacctgccga attggcaggg cgcgctttat
    10741 accacaacac cccccaaaat taaaggcccg agggtttgat ggagtacctt taccttgccc
    10801 tcttaagctt tgccgcgggc agcattttgc ccctgccgtc cgaagcgctg ctcattcctc
    10861 tattacacag cagccagaat ccttggctat tggttgcaac cgccagcatt gccaataccc
    10921 tgggcgccct tactaacacc ctacttggcc gctacctgcg ccatctggcc cacaggccct
    10981 ggttttattt cagccccgca cagatgcagc gggcgcagga ttattttcgg cgcttcggcc
    11041 tgccctgcct gctgtttact tggttgccgc tgctcgggga cttgtttgcc ctggcggcgg
    11101 ggctgctgcg ggcgccctgg ctcatcagta ttgtgctgat cagcctgggc aaaagcctgc
    11161 gttactgctc ggtggcctac ctcgttttgc agacacaagg cgagggttaa ttttgctggc
    11221 aacatcgcct agactggcgc catcccccaa tcgaacccct ccgccatgag ccaatccacc
    11281 cccaccgacc gccaggtcct cagcgtcagc cagttaaacg gccaggtgcg ccagctatta
    11341 gaggcgttgc cgctgttgtg ggtggaaggg gaaatcagca atttatcccg cccctcctct
    11401 ggccactggt acttcaccct gaaggatgcc aatgcgcaaa ttcgctgcgc catgtttaaa
    11461 aaccgcaacc tggccgtgcg ctgcgcggtg aaagacggcg acaaagtact gctgcgggcc
    11521 aaggtgagct tgtatgaggg gcgcggcgac tatcagtt
//
LOCUS       DAOQGS010000052        11426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2626_length_11426_cov_4.885322, whole genome shotgun sequence.
ACCESSION   DAOQGS010000052 DAOQGS010000000
VERSION     DAOQGS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2626_length_11426_cov_4.885322"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..584)
                     /locus_tag="PKZ52_03270"
     CDS             complement(153..584)
                     /locus_tag="PKZ52_03270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG58820.1"
                     /translation="MTQRITDTAWPAHQLAISQLRRAVFIDEQQVPEALEWDDKDEAA
                     RHFCLWQGETLAAYARLLMLEGNTGKVTRMAVSQSQRGVGLGQHLVQHIIAWARQAGL
                     KTLVLEAQVQAQGFYERQQFVASGEVFWDAGIEHRHMALRL"
     gene            complement(565..1689)
                     /locus_tag="PKZ52_03275"
     CDS             complement(565..1689)
                     /locus_tag="PKZ52_03275"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNG58821.1"
                     /translation="MTTPFTHLGSLSVVDFLADYWQKKPLLIRQALPGFISPLAPEEI
                     AGLALEEEVESRLVIETPKAGKPLQSDWHLEHGPLPDERFATLPTSHWTVLVQAVDQI
                     FPAVQELLNQFRFIPNWRLDDVMVSYATTGGGVGPHFDYYDVFLLQGQGRRLWRVGGI
                     VNDATPLREDTDMKILTDFASQEEWVLEPGDLLYLPPNVAHWGIAQSDDCVTYSVGFR
                     APSESEMVLGFSQFVAEELSADQRFRDPEFPLQEDPGEIKPWVIEKFKQHLQALLHNE
                     AQLTEWVGRFATEVKRQSPPSAEVLEGLRLSPACRAAYVLQDDGVLLFVNGELANCSL
                     ALAKAICSYGPIEPEQYGPEDRELIEQMLDAGWLNDSADH"
     gene            1883..1966
                     /locus_tag="PKZ52_03280"
     tRNA            1883..1966
                     /locus_tag="PKZ52_03280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1917..1919,aa:Leu,seq:tag)
     gene            2036..3349
                     /gene="tig"
                     /locus_tag="PKZ52_03285"
     CDS             2036..3349
                     /gene="tig"
                     /locus_tag="PKZ52_03285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNG58822.1"
                     /translation="MQVSLETTSSLERRLTVGVPADVVDGEVNRRLENAARSARINGF
                     RQGKVPMSVVRQRFGQGIRQEVLGDVINRSLDDAFRKENLRPAGRPQIEPKQLGAGKD
                     VEFVAVFEVYPTVELQDLTGQAITRYSAEISETDVDEMIEILRKSQAKWEPVSRPAAT
                     GDRVNIDFVGTQDGAAFDGGSAQGHNLQLGSNTMIPGFEDAIVGLSAGETKDVALTFP
                     ADYHVDSLKGANVNFAITLNSVSAEQLPEVDDIFFATFGITEGGIERFRTDVKNNMEN
                     EKNKASKNRLKNDVFSALLAINPVEVPKSLVNEEIDALRNQALQQYGQLDTSKFDVRA
                     LMPDELFREQAQRRTSLGLLISEIIVKNNLKPDGDRVRSLIESAAASYEDPQGVIEYY
                     YATPNLLASVESAAIEDQVVDLLIGTMQVNDVVVPYQELIRPTAQ"
     gene            3604..4257
                     /gene="clpP"
                     /locus_tag="PKZ52_03290"
     CDS             3604..4257
                     /gene="clpP"
                     /locus_tag="PKZ52_03290"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNG58823.1"
                     /translation="MRDFYLPPSSVQTPQSALVPIVIEQTARGERSFDIYSRLLKERV
                     IFLVGPVEDYMANLVVAQLLFLEAENPDKDIHLYINSPGGSVTAGMSIYDTMQFIKPD
                     VSTMCIGQACSMGAFLLNAGAKGKRYILPNARTMIHQPSGGAQGQASDIHIQAQEILK
                     IRERLNTLMAFHAGRSVEEIARDTERDNFMSAEESKNYGLIDEVLASRDALSKDTKK"
     gene            4374..5666
                     /gene="clpX"
                     /locus_tag="PKZ52_03295"
     CDS             4374..5666
                     /gene="clpX"
                     /locus_tag="PKZ52_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNG58824.1"
                     /translation="MTDHNSDTEDNGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVD
                     LCNDIIREEVHENTSEASSDRLPTPKEISATLDEYVIGQTAAKKVLAVAVYNHYKRLR
                     LGGKKKGKEDVELGKSNILLVGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVG
                     EDVENIIQKLLQKCDYDVEKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLK
                     LIEGTIASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKIIRDRSEKGGIGFSA
                     VVKSKDGSKNIGDSLKSLETEDLVRYGLIPEFVGRLPVVATLEELDKAALVAILSEPK
                     NSLVKQYKKLFEMEGVEIDFREDALRAVAEKAMERKTGARGLRSIMEAVLLDTMYRIP
                     SETGIAKVVVDETVIKGESVPLLVYENAAVPNKRAAEE"
     gene            6030..6944
                     /locus_tag="PKZ52_03300"
     CDS             6030..6944
                     /locus_tag="PKZ52_03300"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNG58825.1"
                     /translation="MPLKIKFFFLMTCVLGLNALAYFYIAEKIHLHQLRAQAQTVVAN
                     VEAFGAWVANSGRVWVKANNSESYLSREEYTVHDGELAQKVVFFSKNPALAQREFADV
                     VMHSSSPTKFRMTSENVMNPNNEPDGFEMRALQAINKGHLNEFTGVYEGAYRYAKPIY
                     HTANCLKCHGDTANAPEDVLKRYGSQHGFGFKEGDLAGIISVSIPTEPLWTNLHQYIG
                     PMEIGLILSTLVLSLVCVHIWVSRPLKQLTTAADNLSLNTGLFNQSTAPLSAAANEIT
                     ALQKSVGRLGTATLHALKKWRDAQQKNT"
     gene            7076..8959
                     /locus_tag="PKZ52_03305"
     CDS             7076..8959
                     /locus_tag="PKZ52_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG58826.1"
                     /translation="MIQLNNLGLQLGTKSLLNNANLTIFPGQKWGVIGVNGSGKSSLF
                     KLLLGQLQADTGEIKIPKDWRLAHMAQEVANSERTALDYILDGDSQYRAIENSIANSE
                     DGERLAALYQQFETIDGYTAPARAARLLSGLGFKPADAQRSVSDFSGGWRIRLNLAQA
                     LMCPSDLLLLDEPTNHLDLDATFWLEAWLQAYKGTLLMISHDRDFLDNCIQAIASFEG
                     QQLISYSGNYSAFERQKAERLAQQNIAFHKQQERMAEIADFVRRFRAKASKAKQAQSR
                     LKELERMEKLAPAHSDSEFSFRFPNPEREVQTLLSLANASLGYEQAVVTKVQVSLFGS
                     SRIGLLGHNGAGKSTLLKSLAGALPLLAGERTESPHLRIGYFAQHQLEALDIHASAAL
                     HLQRLAPKASEQSIRNFLGSFGFIGERALETIEHFSGGEKARLALAIVAWQKPNLLLL
                     DEPTNHLDLEVRQALNDALSAFEGAIVVVSHDRHLLRNTLDEFWLVADGKVAAFEGDL
                     DDYHQWLIAERKTAEETPAAKNSTTDNNKKEVRQHAAARREQLKPITQAIRSIEKKMD
                     LLQKQIQEKETILAEPSLYQQDPTKAQHTAQEVQKLRDELNACEESWLAHQDELERLN
                     TPD"
     gene            complement(9158..9709)
                     /locus_tag="PKZ52_03310"
     CDS             complement(9158..9709)
                     /locus_tag="PKZ52_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58827.1"
                     /translation="MLVNIFNSAEVWHPWIGGYYAWLKSFYHFEPVFRGGYIQHPREI
                     PVTLLSATPQQVTAADDKQAGPADCAAESKAAESKEAGICFWTHEQPHVQDKVVLGID
                     LDNNHFIGKGSVVRVQPENDGNWVCVKIDPEDAFAIRMAEQLCQIEHYRSDNKQYLDR
                     TMSQDEAAQEWIAKYAALFSAHI"
     gene            complement(9812..10375)
                     /gene="rnhB"
                     /locus_tag="PKZ52_03315"
     CDS             complement(9812..10375)
                     /gene="rnhB"
                     /locus_tag="PKZ52_03315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNG58828.1"
                     /translation="MKIAGVDEAGRGPLAGDVVAAAVILDPAKPIKGLADSKKLSEKR
                     REQLFDEIIDKSLAFAIARASPAEIDQINILNASLLAMQRAVDGLALVPDEVLVDGNR
                     LPKWTYPSRAIVGGDSSVEQISAASILAKVTRDRDLCILDQTYPGYGFAGHKGYPTKA
                     HIEALTRLGITPVHRLSYGPVKKLLKP"
     gene            complement(10372..11418)
                     /gene="lpxB"
                     /locus_tag="PKZ52_03320"
     CDS             complement(10372..11418)
                     /gene="lpxB"
                     /locus_tag="PKZ52_03320"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNG58829.1"
                     /translation="MFEGIGGSRMIEQGFKSFFPQDRLAVMGFVEPLKRLPELLRMRK
                     TLADYFVQKIPDVFIGIDSPDFNLHLEMILRNRGIRVAHYVSPSVWAWRQGRIKKIQT
                     AVDLMLTLFPFENAIYERHHIHAVCVGHPLADSIPLDDQTLVARQALGIAPDAQVLAV
                     LPGSRQAEVERLGAMFFEVASRLAADNPALIILCPAANPKRLEQLKVLLAGRDLKVNV
                     QLGNSHQLMAAADAVLMASGTTSLEAMLLKKPMVIAYKVAPVSYWIYKRLVKVSMIGL
                     PNLLAGEKIVPEFIQDAATTDNLCQALGELLRNRGAHQRQKEAFLRLHQSLRRDANQQ
                     AANAIQGLIETSLL"
BASE COUNT         2886 a         3167 c         2837 g         2536 t
ORIGIN      
        1 cccgccgtca gggcctcccc ctgcagggag taaaacccct ccgttatgcg caggctcgcc
       61 tcactcatat atcagtacct tcatttacac acttaaaaag ggcttcggcc ccagctttca
      121 ataacacctg cgcgacaaac acccgcctcg gcctaaagcc ttaacgccat gtgccggtgc
      181 tcgatgcccg catcccaaaa cacctcgccg ctggccacaa actgctggcg ctcgtagaag
      241 ccctgggcct gcacctgtgc ctccaacacc aaagtcttaa ggcccgcctg ccgggcccaa
      301 gcgataatgt gctgcaccaa atgctgcccc aaacccacac cgcgctggga ctggctcacc
      361 gccatgcggg tcactttacc ggtattgccc tctagcatta ataagcgggc ataggcggcg
      421 agggtctcgc cctgccacag gcaaaagtgc cgagcagcct cgtccttatc gtcccactcc
      481 aaagcctcgg gaacctgttg ctcgtcgata aaaaccgccc ggcgcagctg gctgatggcc
      541 aactggtgcg caggccaggc ggtatcagtg atccgctgag tcatttaacc agcccgcgtc
      601 tagcatctgc tcaatcaact cccggtcctc tggcccatat tgttcaggtt cgatgggccc
      661 atagctgcag atggccttgg ccagggcgag ggagcaattg gccagctcgc cattcacaaa
      721 taacagcaca ccatcatcct gcaacacata ggcggcccgg caggcggggc tcagtcgcag
      781 gccctctaac acttcagcgc tgggcggcga ctggcgtttc acctcggtgg caaaccggcc
      841 cacccactcc gtcagctgag cctcattgtg caaaagcgct tgcaggtgct gtttaaattt
      901 ctcgattacc cagggtttaa tctcacctgg gtcttcctgc agtggaaatt ccgggtccct
      961 aaagcgctga tccgccgaca gctcctcggc cacaaactgg ctaaagccca gcaccatctc
     1021 gctttcactg ggggctcgaa agcccacgga ataggttaca caatcatcac tctgggcgat
     1081 cccccaatgg gccacattgg gcggcaagta caacagatcc cctggctcta acacccactc
     1141 ttcttggctg gcaaagtcgg tgaggatttt catatccgta tcctctcgca ggggcgtagc
     1201 gtcattaaca atcccgccca cccgccacag gcgcctgccc tggccctgca gtaaaaacac
     1261 atcgtaataa tcaaaatgcg gccccacgcc gccgccggtg gtggcgtagc tgaccattac
     1321 atcgtccagc cgccagttgg ggataaatct aaattggttt aatagctctt ggacggcggg
     1381 gaagatctga tccaccgcct gcaccaacac cgtccagtgg gaggtgggca aggtggcaaa
     1441 gcgctcatcc ggcagagggc cgtgttccag gtgccagtcg ctctgcaggg gcttgcccgc
     1501 tttaggcgtt tctattacca gccgggattc cacctcttcc tcaagggcga ggcctgcaat
     1561 ctcctccggc gccaggggcg agatgaaccc cggcagggcc tggcgaatca gcaggggctt
     1621 tttctgccag tagtcggcta agaagtccac aaccgaaagt gagcctaggt gagtaaaggg
     1681 agttgtcata agcgccgcgc aaaaaccgcc attgtgcccc atttggctca ttcatcccag
     1741 cacagcggcc aaagcccgtg attttttctt gccatggcct taaacgctgt ggcagaatcc
     1801 tgctccctca aatttgggat ggcggatgcg cggcaattgc gcactagagc cccatcacag
     1861 cgttctgagg tgttcatagc gagcggtcgt ggcggaattg gtagacgcac tgggtttagg
     1921 ttccagcgcc tttggtgtga gagttcgagt ctctccgacc gcaccactat aaacacctcc
     1981 ctcttaaatc atccgaatta cacaaaatta ctgaatttac ttaacgaggc acagcatgca
     2041 agtttcgctt gaaacaacat caagtctgga gcgccgcctg accgtaggag tccctgcgga
     2101 tgtggttgat ggcgaagtaa accgccgcct agaaaatgct gccagaagcg cacgcatcaa
     2161 cggcttccgc caaggcaaag tgcccatgag tgtcgtgcgt cagcgcttcg gccaaggcat
     2221 ccgccaggaa gtcctcggcg atgtcatcaa ccgctctttg gatgacgcct tccgcaaaga
     2281 aaacctgcgc cccgctggcc gcccccaaat cgaacccaaa caattaggcg cgggcaaaga
     2341 cgttgagttt gtcgccgtct ttgaagtcta tcccaccgtt gaactgcagg atttaactgg
     2401 ccaagcaatc actcgctact ccgccgagat cagcgagacc gacgtggatg agatgatcga
     2461 aatcctgcgc aaaagccaag ctaaatggga gccagtttcc cgccccgccg ccaccggtga
     2521 tcgcgttaat atcgactttg tcgggaccca agacggcgcc gccttcgacg gcggcagcgc
     2581 ccagggccac aacttgcaac tgggctctaa caccatgatc ccaggctttg aagacgccat
     2641 agtgggcttg tccgctggcg aaaccaaaga cgtcgcgctc accttcccag ccgactacca
     2701 cgtagatagc cttaagggcg ccaacgtcaa ttttgccatc accctaaata gcgtctcagc
     2761 cgagcagcta cccgaggttg atgacatctt cttcgccacc tttggcatca ccgaaggcgg
     2821 catcgagcgc ttccgcaccg atgttaaaaa caacatggaa aacgagaaga acaaggcgtc
     2881 caaaaaccgc ctgaaaaacg acgttttctc cgctttgctg gctattaacc cagtggaagt
     2941 tcccaaaagt ctggtcaacg aagaaatcga cgccctgcgt aatcaggcgc tacagcagta
     3001 cggccagctg gataccagta aattcgacgt gcgcgccctg atgccggacg aactctttcg
     3061 cgaacaagcc cagcgccgca cctctctggg ccttttgatc tcggagatca tcgtcaaaaa
     3121 caacttaaaa cccgatggcg atcgcgtacg cagcctgatt gaaagcgccg ccgcttccta
     3181 cgaagaccct cagggcgtaa ttgaatacta ctacgcgacc cccaacctgt tggccagcgt
     3241 tgaatctgca gcgattgaag atcaggtggt ggacctcctg atcggcacca tgcaggtcaa
     3301 tgatgtggtt gttccatatc aagaattgat tcgtcccacc gcgcaataat ctgcctctgc
     3361 tggcggcgag ccctaagctc ccgccagcgt ttacccgctt ttaacaggcg gttgagcggc
     3421 acttttttgt cagggataca gcctcttgac tacagaataa tcgccccaat ggcgcggttg
     3481 tggctacgga ctttttctgg aatatctttg tttctcgggt taagcttgga tgtgctcagc
     3541 catctcacac accagccgat agcggactgt aaaccttaag acagaaccaa ggatatcctt
     3601 gatatgcgcg atttttacct accaccctct tccgtacaaa cgccccaatc tgccctagta
     3661 cccatcgtaa ttgagcagac cgcacggggt gaacgctcct ttgacatcta ctcccgcctg
     3721 ttaaaagagc gcgtgatttt tttggtgggc ccagttgaag actacatggc caacctggtt
     3781 gtggcccaat tgttattttt agaagccgaa aaccccgata aagacattca tctatatatc
     3841 aattcaccgg gcggctcggt aaccgcgggc atgtcgattt acgacaccat gcaatttatc
     3901 aagcccgatg tcagcaccat gtgtatcggc caggcctgca gcatgggcgc gtttttgctc
     3961 aacgccggag ccaagggcaa acgctacata ctgcctaacg ctcgcaccat gattcaccag
     4021 ccctccggcg gcgctcaggg ccaggcgtct gacattcata ttcaagcaca agagatttta
     4081 aaaattcgcg agcgcttgaa taccctgatg gcgtttcatg ccggccgcag cgttgaagaa
     4141 attgcccgcg acaccgagcg cgacaacttc atgagcgccg aggagtcgaa aaactacggc
     4201 ctgattgacg aagtgctggc aagccgtgac gcacttagca aagatacgaa aaaataaacc
     4261 agcgcgcagt tcaaagtgga tatgcgcgca accaatgggt ttaaaagagc gattcgggca
     4321 ccatataagc ctgctatttt tgacccaatt ttttaatttg gcggagaggt ttaatgaccg
     4381 accacaacag tgataccgaa gacaacggca agctgctgta ctgctcattt tgtggcaaga
     4441 gtcagcacga ggtgcgcaag cttattgctg ggccgtccgt atttatttgc gacgagtgcg
     4501 ttgacctgtg caacgacatt attcgcgaag aggtgcatga aaacaccagc gaggccagca
     4561 gcgaccgttt gccgacgcca aaggaaattt cagccaccct cgatgaatac gtaatcggtc
     4621 aaacggcggc gaaaaaagtc ctcgccgttg cggtgtacaa ccactacaag cgcctgcgct
     4681 tggggggcaa gaaaaaaggc aaagaggatg ttgaactcgg caaaagcaac atcctgctgg
     4741 taggtcccac cggtagcggt aaaaccctcc tggcagagac gctggctcgc ctgttaaacg
     4801 tgcctttcac catcgccgat gccaccaccc tcaccgaagc cgggtacgtg ggtgaagacg
     4861 ttgaaaacat catccagaag ctgctgcaaa agtgtgatta cgacgttgaa aaagctcagc
     4921 agggaatcgt atacatcgac gagatcgaca agatttctcg caaatccgac aacccgtcga
     4981 ttacccgcga tgtatccggc gaaggtgtac agcaggcgct cttgaagctc attgaaggca
     5041 ccattgcttc tgtgccaccc cagggcggtc gcaagcaccc acagcaggag tttttacagg
     5101 tcgataccag caatattctg tttatttgcg gcggcgcatt tgccggtctc gacaagatca
     5161 tccgcgatcg ttccgaaaaa ggcggtatcg gcttttcagc tgtagttaag agcaaagacg
     5221 gctcgaaaaa catcggcgac tctttgaaga gcttggaaac cgaagacttg gtacgctacg
     5281 gcttaatccc tgagtttgtc ggccgcttac ccgtggttgc aacccttgag gagcttgata
     5341 aggccgccct cgtggccatt ttaagcgagc ctaaaaactc cctggttaaa cagtataaaa
     5401 aactgtttga aatggaaggc gtagaaattg attttcgcga agacgctctg cgcgctgtgg
     5461 ccgaaaaagc catggagcgc aaaaccggcg ctcgcggcct gcgctccatc atggaagcgg
     5521 tgttgctgga taccatgtac cgcattccat cggagacggg tattgccaag gtagttgtgg
     5581 atgaaaccgt cattaagggt gaatccgtgc ctctgttggt ttatgaaaat gccgccgtgc
     5641 ctaataagcg cgccgccgag gagtaaatca tcgacacatc aaggccctac ggggcctttt
     5701 ttataggtgt gcttttttaa gagtgtaata aacactttca gctttaccta aaaatccctc
     5761 ccgtcattaa acctgcgtat ttcctggata ggccccggca cctctcttag cgggttcccc
     5821 aaaaacaact tctaacaaag cttgcggcag gattacagca aatgtacgga aatgtcgatt
     5881 cgtccgcgca aactgtgcct acacttagac gcatcagaca ttctgtcccc tatttaaaaa
     5941 tggaagtcgt gcatttggtt ttatactatt aatttagtga ttttagactg tgggctgcag
     6001 ggcgggcagc cagttgcgga gattacgacg tgccgttaaa aattaaattc ttctttctaa
     6061 tgacttgtgt tttaggcctg aatgcactgg cttattttta tatagccgaa aaaattcacc
     6121 tgcaccaact gcgtgcccaa gcgcaaacag tggttgccaa tgtagaggcg tttggcgctt
     6181 gggtcgccaa ttccggccgt gtgtgggtaa aagccaataa ttccgagagt tatttaagcc
     6241 gcgaagaata tacggtacac gatggtgaat tggcgcaaaa ggtggttttt ttcagtaaaa
     6301 accctgcctt ggcgcaacgc gagtttgccg atgtggtaat gcactcgtca tcgcccacta
     6361 aatttcgcat gacatcggaa aatgtcatga acccgaataa tgaacccgat ggttttgaaa
     6421 tgcgtgcatt gcaggcgatt aacaaaggcc atttaaacga atttaccggt gtttatgagg
     6481 gcgcctaccg ctacgccaaa ccgatttacc acaccgccaa ttgccttaaa tgccatggcg
     6541 ataccgccaa tgcaccggag gatgtgctca agcgttatgg cagccagcat ggttttggtt
     6601 ttaaagaggg tgatctggca gggatcatca gcgtttctat acccactgag ccgctctgga
     6661 ccaatctaca tcaatacata ggccccatgg aaataggctt gattttaagc accttagttc
     6721 tgagccttgt gtgcgtgcat atttgggtca gccggccact taagcaactc accactgcag
     6781 cggataattt atccttaaac accggcttgt ttaatcaatc gactgcgcca ctttccgctg
     6841 ccgccaacga aataacagcg ctgcaaaaat ctgtggggcg cttgggtact gccaccttgc
     6901 acgccctaaa aaaatggcgc gatgcccagc aaaaaaatac ctaagccggt tgcgctgatc
     6961 ggcaagcgcc cccaatagtc agccatcgaa atttgacagt aaatacggca ggcaaaaacc
     7021 tgcggctttg gtgtactctt gccgccctcg cccactttag ccgctattaa ttcccatgat
     7081 tcaattgaac aaccttggcc tgcaactggg caccaagagc ctcttaaaca acgccaatct
     7141 aacgattttc ccaggacaaa aatggggggt cataggtgtt aatggcagcg gtaaatccag
     7201 cttatttaaa ttattactcg gccaattaca ggcggacacg ggcgagataa aaatccctaa
     7261 ggactggcgc ttagcccaca tggcccaaga ggtagccaat tctgagcgca cggcactgga
     7321 ctatattctc gatggcgaca gccagtaccg cgctatagag aactctatag ccaacagtga
     7381 agatggagaa cggctcgcgg ccctgtatca acagtttgaa accatcgacg gctataccgc
     7441 ccctgcccgc gcggcgcgtt tattatcggg tttgggtttt aaacctgccg acgcccagcg
     7501 cagcgtcagc gatttttccg gcggctggcg catccggcta aatctagccc aggcgcttat
     7561 gtgccccagc gatttattat tgcttgatga acccaccaac cacttggatt tagacgcaac
     7621 cttttggctg gaagcttggc tgcaagccta caaaggcaca ctgctgatga tttcccacga
     7681 ccgggatttt ctcgacaact gcatccaagc catcgccagt tttgaaggcc agcaattaat
     7741 cagttacagc ggtaattatt cggcctttga gcggcaaaaa gccgaacgtt tggcgcaaca
     7801 gaatattgcc tttcacaaac aacaagagcg catggcggaa attgccgatt ttgtacggcg
     7861 ttttcgcgcc aaagccagca aagccaaaca ggcgcaaagc cgcttaaaag aattggagcg
     7921 catggaaaaa ttggcgcccg cccacagcga ctcggaattt agttttcgtt tccccaaccc
     7981 cgagcgcgaa gtgcaaaccc tcttaagcct tgcaaacgca tcattgggct atgagcaggc
     8041 ggtggtaacc aaagtgcaag tttcgttatt tggcagcagc cgtatcggtt tactggggca
     8101 caatggcgcc ggtaaatcca ccctgcttaa atccctagcc ggggcgctgc cgttactcgc
     8161 cggtgaacgc acggaaagcc cgcatctacg catcggctat tttgcccaac accagttgga
     8221 agccttggat attcacgcca gtgcggccct gcatttacaa cgcctggcac ccaaagccag
     8281 cgaacagagt attcgcaatt ttttaggcag ctttggcttt atcggcgagc gcgccttgga
     8341 aaccatcgaa cacttctccg gcggtgaaaa agctcgcttg gcgctagcaa ttgtagcctg
     8401 gcaaaaaccc aatttattat tgctcgacga acccaccaac catttagatt tagaagtgcg
     8461 ccaggccctt aacgatgcct tatcagcatt tgaaggcgcc atagttgtgg tctcccacga
     8521 ccgccattta ctgcgcaata cgctggatga attttggttg gtggccgatg gtaaggtggc
     8581 agcttttgaa ggcgatttgg atgattacca tcaatggtta atcgccgagc gcaaaacagc
     8641 tgaggagaca ccggcggcaa aaaattctac gaccgataat aataaaaaag aagttcgcca
     8701 acatgcggcg gcgcgacggg agcaattaaa acccataacc caggcaattc gcagcatcga
     8761 gaaaaaaatg gatctgctgc aaaaacagat tcaggaaaaa gaaactattt tggccgaacc
     8821 aagcctgtat caacaagatc caaccaaggc ccagcacacc gctcaagaag tgcaaaaact
     8881 gcgcgatgaa ttaaacgctt gtgaggagtc ctggctggcc catcaagatg aattagagcg
     8941 cttaaatacg cccgattaaa acgcaaggaa tttacacagt aaaatcccgc tgcgaacacc
     9001 taaagttttc aggattgaaa aaagaattag cgacacaaaa cggccgccaa agagaaggcg
     9061 tacgaaagac gaggcaagct aaagggacgg acaaggaggc agtagagcga agagacaaca
     9121 acaatacagc ggcaactaac aggcttggca ggcgcagtta aatatgggca gaaaataaag
     9181 cggcatactt agcgatccac tcctgggcgg cctcatcctg cgacatagtg cggtctaaat
     9241 actgtttgtt gtcgctgcgg tagtgttcaa tctggcataa ctgctcggcc atacgaatgg
     9301 caaacgcatc ttcaggatca atttttacgc atacccaatt gccgtcgttt tcaggctgca
     9361 cgcgcacaac actgcctttg ccaatgaagt ggttattgtc cagatcaatg cctagtacca
     9421 ccttatcttg cacgtggggt tgttcatggg tccaaaaaca aatgccggct tctttactct
     9481 cagctgcttt actctcagca gcacagtctg cagggccggc ttgtttatca tcggccgccg
     9541 tcacctgctg gggagtggcc gataaaagtg tgactggaat ttctcgcggg tgctgaatat
     9601 aaccgccgcg gaaaacaggc tcaaaatggt agaagctttt cagccaagcg tagtaaccac
     9661 caatccaagg gtgccacacc tcggcactgt taaatatatt aactaacatc cagataaccc
     9721 caacaatgac tcgcactgtc aatcaccgat atctaatgta tagctaattt tgtgccggaa
     9781 cttattttct tttaagcggt gcgaccaacg gttagggctt gaggagtttt tttaccggtc
     9841 cataggataa gcggtgtacc ggggtgatgc ctaacctggt taaagcctcg atatgggcct
     9901 tggtggggta gcctttgtgg cctgcaaagc cataacctgg gtaggtttga tcgaggatac
     9961 ataggtcgcg atcgcgggtt accttagcca aaatcgacgc ggcgctaatt tgttcgacac
    10021 tgctgtcacc accgactatg gcccgcgatg gataagtcca cttcggcaag cggttgccgt
    10081 caactaatac ttcatctggc accagtgcaa gcccgtccac ggcccgctgc attgccagta
    10141 aactggcgtt aagtatatta atttgatcaa tttccgcagg gcttgcccgg gcgatggcaa
    10201 acgccagtga cttgtcaatt atttcgtcaa acagctgttc gcggcgtttt tcactgagtt
    10261 ttttcgaatc cgccaggcct ttaatgggtt ttgcgggatc taaaataacc gcagcggcca
    10321 ccacatcacc cgccagcggc cctcgcccag cttcgtcaac accggcaatt ttcatagcaa
    10381 agaggtctct attaagcctt gaatcgcgtt ggccgcctgt tgattggcgt cgcgccgcaa
    10441 actttgatgc aggcgtaaaa aggcttcttt ttgacgctga tgggcgcctc tattgcgaag
    10501 cagttcaccc agggcctgac acaaattgtc agtggttgcc gcatcttgta tgaactcggg
    10561 gacaattttt tccccggcca ataaattcgg caacccgatc atactcacct tcacaagacg
    10621 cttgtaaatc caataactca caggcgctac cttgtaggca attaccatgg gttttttcag
    10681 caacatggcc tcaaggctgg tggtgccaga tgccatcagt accgcatcgg cagccgccat
    10741 taactggtgg gagttgccca gctgcacatt aactttgagg tctcgccctg ccaacagcac
    10801 tttcaactgc tccaggcgtt taggattggc ggcggggcaa agaataatta acgctggatt
    10861 gtccgccgcc aaccggctgg ccacttcaaa aaacatagcc cccaagcgct ccacctccgc
    10921 ttgacggctg ccaggtaata ccgctaaaac ctgcgcgtca ggcgctatgc ccagggcttg
    10981 ccgggcgact aaggtttgat cgtccaaggg aatcgaatcc gccagcgggt ggccgacaca
    11041 tacagcatgg atgtggtggc gctcgtaaat ggcattttca aacggaaaca gggtaagcat
    11101 taaatccacc gctgtttgta tttttttaat gcgcccctgc cgccaggccc aaacggatgg
    11161 gctgacataa tgcgccacgc gaatgccccg gttgcgcagg atcatttcca aatgcaaatt
    11221 aaaatccggc gaatcaatac cgataaatac atccggtatt ttctgcacaa aataatcggc
    11281 gagggttttg cgcatgcgca gaagttccgg caggcgcttt aagggttcca caaagcccat
    11341 taccgctaaa cgatcctgcg gaaaaaaact tttaaacccc tgctcgatca tacggctgcc
    11401 accgatacct tcaaaaatgg catccg
//
LOCUS       DAOQGS010000053        11314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2647_length_11314_cov_4.545430, whole genome shotgun sequence.
ACCESSION   DAOQGS010000053 DAOQGS010000000
VERSION     DAOQGS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2647_length_11314_cov_4.545430"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="PKZ52_03325"
     CDS             complement(<1..1380)
                     /locus_tag="PKZ52_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1595 domain-containing protein"
                     /protein_id="HNG58830.1"
                     /translation="MKKILPLLIASLVAAGCSGTGPSSSSSSSGSSSSTSSSSSSGST
                     SSSSSSSSSSSSSSSSSRPSSSSTSSSSSSTSSTGGGTGDVAAGKAVVKSACLGCHKD
                     PEEDGKFIGVFSFDVNRFTYHTMDDYQGKGYTGSSQSDLANFIGNEMLGCGGACTDAQ
                     NAAAYLWSIRGQAPVVTQYACDTSAPVLYGRRDLKLLTSYEYHNSLQALFKKPLPDDF
                     STQNRASDDIKVGRMPNHTLEKVSEGRLTAYDNNAEELAKWALATTGALDFDCLDKTT
                     CANNFIDKFAYYAFRRPLTSAERTEYRDIIGTPKDVKTGLQWAIRSVLMSPQFLYRSE
                     LGKTVAEARTYKPEQPTDADVVQGEPLTTITMPEVTYGTDGFATYGAYKYVGLTPSYT
                     WTGDDIVVVSVKSGGSGNGNFGITINNGTFKQETVVDSNKTITLRVKGITGAGKYVQT
                     YNQSGNAIGV"
     gene            complement(1868..2767)
                     /locus_tag="PKZ52_03330"
     CDS             complement(1868..2767)
                     /locus_tag="PKZ52_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNG58831.1"
                     /translation="MQPISLTTRYVPMFKSTVLKSLNYLALGCSCITLLACSHKPQFT
                     LQQVWKTAGFLVPESVLLVGEGAQQRIFVSQIDGQGGDADGKGAIAEINSDGALIDAN
                     WISGLNAPKGMAAFNNRLYVADLTELVIIDIAQKQIIQKLPLPQAKFANDVAVDKNGV
                     VYISDTMAHRVYKFADGNASVLVKDTDNANGLWVTDSGLMINCAEELKFYDFQQQKLT
                     TLESGFSKNLDGIAALDDKHFLLSVWAGKIYEYTLGGGKAVLLDSTEQKINTADIALD
                     QKSRVLYVPNFLNNTVTAYKVLP"
     gene            complement(2890..5208)
                     /locus_tag="PKZ52_03335"
     CDS             complement(2890..5208)
                     /locus_tag="PKZ52_03335"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNG58832.1"
                     /translation="MKTYLLGLSISCALLGCSGGKPTEQTKVDYNQLKATTQSDTRLD
                     TPSKTTTEAHLKNGLRLQLNQSNTYLRDSVTAGGATGNPVPVSSPESTNFSATNVHIA
                     GVDEADVAKYDGQYWYWAANYQQGMRIFKAGSDSKAELISEQAATPQLTTQGIYLQQT
                     QGKTTHVVSLNSEYGNIGPIWLPAINVGIAQPAIATDALIARPFYYRPQNGKLVIEWS
                     DVSQVAKPAVTRQLTLQGRLMDSRKIGDVLYVFTRFDPWLDELKPAELNSAQQDANEQ
                     ILQKTALVDLMPKYNFDQQAQKPLTDNCMVPAGVDSHQGFPSLVHITAVDLKTQTLLG
                     STCINAPMQSFTMNEQHAFLTAHAAGLNGVNKTFIHQFTLSEKGASYSASGSVAGFIN
                     GDLSFWLNEKDGYVRVLSSDLSNQDTQHRISVLKEQKGELTTLAQLPNTQHPEKIGKP
                     GESIYGARFVGDKAYVVTFRRTDPLYVVDLKTPSEPFIAGELQVPGFATYLHPVGENY
                     LFTLGQSADDTGRVTGIKTELIDVSNPAKPISTKALYFGTNASNADALYDLHAVAVLP
                     QTNGDTRFAFSINNYDQNYQWAYSGVYLFSVAGVANNKADLTHDGVVITEARSSQQYA
                     PKYYGNGRSLLNGDAVYYGVGGSVWASNWNTPKQVTGPIPAPKPQPQPDPIACTADVR
                     AGLDIHVYTSGAAACDTSIEIQDGDYRERPTGYYPGDSRTECVYSGAAERAGSYSITA
                     KLKGFATQTLQTQVSSDRCHVITEQVTIKLAP"
     gene            5487..6203
                     /locus_tag="PKZ52_03340"
     CDS             5487..6203
                     /locus_tag="PKZ52_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNG58833.1"
                     /translation="MRIIDMYVCRLAEFSDLDGEVAAQFLDVQEHARFSRFKFDRHRQ
                     AFLASRVLQKTALAQRLKCPIADLAFDFGPQGKPALQRGINPGGVEFNLTHSDTWALL
                     AVDQYPLGIDTENMQRNTNILSIARHNFHPREIAFLTAQAAEPQWGFYYWMLKEAYIK
                     YLGTGLSQSLKEFYFTWQPLQFGSETPLPVPAACVFNWAADAVAALCYQPGEVHLRLQ
                     QLSPQGTWTPLEIQLLVQTQ"
     gene            complement(6319..6963)
                     /locus_tag="PKZ52_03345"
     CDS             complement(6319..6963)
                     /locus_tag="PKZ52_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNG58834.1"
                     /translation="MSVQIIGVPFSNLVRSVLLCCEEKNIPYSLSMQHQGQPIGLKSA
                     ALNALNPIGKIPVLVHDDFVLSETQSILRYLDAQFPAPGLQGKTPRERALIDQWCAVF
                     NQQSDLIFVRHYLLEWVFPKGENGQLRQDVIDNATPAVKEHLALLSAALGTQHFWVGD
                     ALSLADLLLLPMLDYLLALPNGPALFAAAENLSAYTQQMRQRISSSKILQAPKG"
     gene            7123..7689
                     /locus_tag="PKZ52_03350"
     CDS             7123..7689
                     /locus_tag="PKZ52_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG58835.1"
                     /translation="MGWSAHHKTASRETILLAAAELFARYGYDAVAIDRVMAQAGMTR
                     GAFYAHFTSKSDLYAQAMLLAGQRLVAHSLHLGMNFEEFVCTYLTPGQLAMAPLACPL
                     ACLVTDVAPREERVKIVYGQLLAGFVSHLQRLNPRLTQARAAACASSLIGAQVVARAV
                     HTQELAKNILLSAQHTILEHFADNKNEA"
     gene            complement(7730..7876)
                     /locus_tag="PKZ52_03355"
     CDS             complement(7730..7876)
                     /locus_tag="PKZ52_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58836.1"
                     /translation="MRTDVLSLAAIIFVVGVLISGLGVTNVLHKKTTPSAELQQGFAL
                     TQPE"
     gene            complement(8066..9610)
                     /gene="ilvA"
                     /locus_tag="PKZ52_03360"
     CDS             complement(8066..9610)
                     /gene="ilvA"
                     /locus_tag="PKZ52_03360"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNG58837.1"
                     /translation="MAAFASLTMPQSYIKRILNARIYDLAVETPLEFAPLISERCQNQ
                     VWLKREDLQPVFSFKIRGAYNKLLQLTPEQLAKGVVAASAGNHAQGLALAAAHLGVKA
                     TIVMPRTTPAIKVNAVKRRGATVELQGDSYDEAAAFAKQLVQQQGMVYIHPFDDPDVI
                     AGQGTVAMEILRQHPHPLDAVFIPVGGGGLCAGMAAYIKYLRSDVKVFAVESEDSACL
                     AAAMASGERSVLSQVGLFADGIAVAQIGEETFRVLRECIDGVITVSSDEICAAIKDIF
                     EDNRSITEPAGAAALAGLKKYVAREGVSGQSFIAIDSGANTNFDRLRYISERTEISEN
                     REAILAVTIPERPGSYLEFCTLLGRRAITEFNYRFQNAQENAHIFVGLQISPERGDRQ
                     DLIQRLRQQGYEVTDLSDNEMAKLHIRHMVGGRAPGDLSDELLMRFEFPERPGALLQF
                     LTKLAGRWNISLFHYRNHGAAFGRVLVGMQVPADSRQALITALEELGYPFWDESQNPA
                     YRLFLA"
     gene            9739..10569
                     /locus_tag="PKZ52_03365"
     CDS             9739..10569
                     /locus_tag="PKZ52_03365"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNG58838.1"
                     /translation="MLLSSPLVNVAWLSEQLDAANLVLLDASMAPPGQPTYAPVQVIP
                     GARRFDFDKQVCDQNSALPHMMPAAEQFTEAAQALGINSDSTIVVYDHLGLFASPRAW
                     WMFKSMGFDAVYVLDGGLPAWVLAGLPTLERYSPALTPGNFTARYSADYFVDKTQVFA
                     ALDNPAISILDARPSGRFAGTQAEPRPGLRSGHMPGAFSLPFTAVQSGGQLLGAQQLA
                     ELLGAPRPLICSCGSGVTACVLALAAVVAGFREVAVYDGSWSEWGDPQWQLPVVTESA
                     "
     gene            10677..11006
                     /locus_tag="PKZ52_03370"
     CDS             10677..11006
                     /locus_tag="PKZ52_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58839.1"
                     /translation="MKKFHLALGVADIAASVEDYSERLQSQPALVVPGEYALWRTAHL
                     NFSVRKVAAEQVGQLRHLGWEDPHAEAFSCSEDCNGVGWEHFNLQQQLDEIALAWPGS
                     VKGSGRV"
     gene            11099..>11314
                     /locus_tag="PKZ52_03375"
     CDS             11099..>11314
                     /locus_tag="PKZ52_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNG58840.1"
                     /translation="MTLILGIDPGSRKTGYGLILQQGQRLNYCASGVIRPPEDAPLSE
                     RLKVIFDGISLLIAQHSPAQFAIEQIFM"
BASE COUNT         2474 a         2844 c         3183 g         2813 t
ORIGIN      
        1 caccccaata gcgttgccgc tctgattgta ggtttgcacg tatttaccgg caccggtaat
       61 gcctttcacc cgcagggtaa tggtcttgtt gctatccacc acggtttcct gtttgaaggt
      121 gccgttgttg atggtgatac caaagttgcc attgccggag ccgccggatt taaccgacac
      181 caccactata tcgtcaccgg tccaggtgta gctgggggtg aggcccacat acttataggc
      241 gccgtaagtg gcaaagccgt ctgtgccgta ggtcacctcg ggcatggtaa tggtggtgag
      301 gggttcaccc tgcactacgt cggcgtcggt gggttgctca ggcttgtagg tgcgcgcctc
      361 ggcgacggtt ttacccagct ccgagcggta gaggaattgc ggtgacatca acaccgagcg
      421 aatggcccat tgcaagccgg ttttgacatc tttaggtgtg ccgataatgt cgcgatactc
      481 cgtgcgctca gccgacgtca gcgggcggcg gaaggcgtag taggcgaatt tatcgataaa
      541 gttattggcg caggtggttt tgtctaggca gtcaaaatcc aaggcacctg ttgtggccaa
      601 cgcccacttg gccagttctt cggcgttgtt gtcataggcg gtcaagcggc cttcagacac
      661 cttctccaac gtatggttgg gcatgcggcc cactttgatg tcgtcactgg cgcggttttg
      721 ggtggagaaa tcatccggca ggggcttttt aaacagcgcc tgcagggagt tgtggtattc
      781 gtaggaggtc aacagtttta aatcccggcg gccgtacaac acaggcgcgc tggtatcaca
      841 ggcgtattgg gtcactaccg gcgcttggcc gcgaatggac cataaataag cggcggcatt
      901 ttgtgcatcc gtacaggctc cgccgcagcc gagcatttca ttgccgataa aattggccag
      961 atccgattgc gacgaacccg tatagccctt gccttgataa tcatccatgg tgtggtaggt
     1021 aaagcggtta acgtcaaaac taaacacgcc tatgaatttg ccgtcttcct ctggatcttt
     1081 atggcaaccc aaacaggcgg atttaaccac tgctttacct gcggccacat cacccgtgcc
     1141 gccaccggtt gaggaggtgg agcttgatga agacgaggtg gacgagctgg agggccgcga
     1201 tgacgacgag ctggatgaac tcgacgaact ggatgagctg gatgaacttg tcgagccgga
     1261 ggagctagat gaacttgtcg agctagaaga gccggaagac gacgaagagc tggacgggcc
     1321 agtccccgaa cagccggcgg ccaccaggct ggcaataagc agaggaagaa tttttttcat
     1381 agtgtttccc ttaatctagt ctgtgccatt tacccttcgg cttaagccaa tgcgggaggc
     1441 aagacacata tttataggta attcagcaac aatattaagt gtctgggcgg cccccagcgg
     1501 ctggcctgaa tgagcgttga ggcgtttggc agtggggtaa tttcccgcat ttaaatgggg
     1561 ctactccgct gaccaaggcg tgtaaaaacg cccgctcggt aaattcaggc tcaaacctag
     1621 agtaaccgat tgcagggggt gaaaatggta atagtcgatg tttctagaag ctaccttggg
     1681 gtagttgaat gaattttctg gggggttgat cttagttaaa gaaaagcgcc gagtgagcgg
     1741 cgctttttag gttaataacc cgtggctgca ggttctgtca gatggggaaa atacacctgt
     1801 attgcggcct aaattctgct gattctattg cggcaccgcg taaaaaacgc aacggctaag
     1861 gccagtgtca gggcaatacc ttgtaggctg tcactgtatt gtttaaaaaa ttcggcacgt
     1921 ataacacccg cgatttttga tccagcgcaa tatcggcagt attaattttt tgctctgtcg
     1981 aatcgagcaa aactgccttg ccgccgccga gggtgtattc gtaaattttc cccgcccaca
     2041 cgctcagcag aaaatgttta tcgtccagtg ccgctatgcc gtccaggttt ttgctaaagc
     2101 cgctctccaa ggtggtgagt ttttgctgct gaaaatcgta aaactttaat tcctcggcac
     2161 aattaatcat cagcccactg tcggtaaccc ataagccatt ggcattatcc gtatctttta
     2221 ccaatacact ggcattgcca tcggcaaatt tgtatacccg gtgggccatg gtgtcggaaa
     2281 tatacaccac gccgttttta tccacggcca catcattggc gaattttgcc tggggcaagg
     2341 gcagtttttg gatgatttgc ttctgcgcaa tatcaatgat aaccagctca gttaaatcgg
     2401 ccacatacaa gcggttatta aacgcggcca tgcctttggg ggcatttaag ccgctgatcc
     2461 aattggcatc tatgagcgcg ccgtcggaat taatttcagc aatcgcgcct ttgccatccg
     2521 catcgccgcc ttggccgtct atctgggaga caaaaatgcg ctgttgtgcc ccttcaccca
     2581 ctaataaaac ggattctggt actaaaaaac ctgctgtttt ccacacttgt tgcaaggtaa
     2641 attgcggttt atggctgcag gcaagcagcg ttatacagct gcaaccgagg gcgaggtaat
     2701 tgagagattt aagtacagtg gatttaaaca tgggcacata ccgagtggtg agcgatatag
     2761 gctgcacagt gtactgcact taaacgggta atcggcttat aggccgaata caaaaagccc
     2821 ggcacatggc cgggcaaagt tattaacccg gcggcatttg gtgccgggtt aatacatggc
     2881 gcctattttc tacggtgcaa gtttaatcgt gacttgttct gtaatcacat ggcagcgatc
     2941 actgctcacc tgtgtttgca gggtttgggt ggcaaaaccc tttaatttag cggtgatgct
     3001 gtagctgcca gcgcgttcag ccgcgccgga atagacacat tctgtgcggc tgtcgccggg
     3061 atagtagccc gtggggcgct cgcgataatc accgtcctga atttcgatgc tggtatcaca
     3121 ggcggcagcc ccgcttgtgt acacgtgaat atccaggccc gcgcgaacat cggcggtgca
     3181 ggcgatggga tccggttgag gttgtggttt aggggcggga atcgggccgg tcacttgttt
     3241 gggggtgttc cagttgctgg cccagacgct gccgccgacg ccgtaataaa ccgcatcacc
     3301 gtttaacagg ctgcggccat tgccgtaata tttcggcgca tattgttggc ttgagcgcgc
     3361 ctcggtaata acgacaccgt cgtgggttaa atcggcttta ttgttggcaa cgcctgcaac
     3421 cgagaataaa taaacgcccg aataggccca ctgataattt tggtcgtaat tattaatgga
     3481 aaaggcaaag cgtgtatcgc cattggtttg gggtaatacg gctactgcgt gtaaatcgta
     3541 gagtgcatcg gcattgctgg cgttagtgcc aaaatacaga gctttggttg aaatcggttt
     3601 ggccgggtta ctcacatcaa ttaattcggt tttaatgcct gttacacggc cggtgtcatc
     3661 ggcactttgg ccgagcgtaa acagataatt ctcccccact gggtgcaagt aggtggcaaa
     3721 gcctggcacc tgtaattcac cggcaataaa aggctcactc ggtgttttta ggtcgactac
     3781 atacagcgga tcggtgcggc ggaaggtgac cacataggct ttatcaccca caaaacgggc
     3841 gccgtaaata ctctcgccgg gtttgcctat tttttctggg tgttgtgtgt tgggcagttg
     3901 agcaagcgtg gttaattcgc ctttttgctc ttttaagaca ctaattctat gttgggtatc
     3961 ctggttgctt aaatcgctgc tcaacacccg cacataaccg tctttttcat ttaaccaaaa
     4021 cgataaatcg ccattgataa aaccggccac actgccgctg gcggaataac tggcgccttt
     4081 ctcgctcagg gtaaattggt gaataaaggt tttattcacg ccgttaaggc ccgcggcatg
     4141 ggcggttaaa aaggcgtgtt gttcattcat ggtaaaactc tgcatgggcg cgttaataca
     4201 ggtgctgccc aagagggttt gagtttttag atccacggcg gtgatatgca ccaggcttgg
     4261 aaagccctgg tggctgtcaa cccctgcggg caccatacaa ttatcggtga ggggtttttg
     4321 cgcttgttgg tcgaagttgt acttcggcat taaatcaacg agtgcggttt tttgcagaat
     4381 ttgttcgttg gcatcttgct gggcggagtt aagctcagcc ggttttagct catccagcca
     4441 ggggtcaaag cgtgtgaata catacaacac atcacctatt ttgcgcgagt ccatcaagcg
     4501 cccttgcagg gttaattgcc gtgtgacggc aggcttggcg acttggctga catcgctcca
     4561 ctcgatcact aatttgccat tttgcgggcg gtaataaaaa ggccgcgcaa tcagggcatc
     4621 ggtggcaatc gccggttgcg ctatgcccac attaatggcg ggcagccaaa tagggcctat
     4681 gttgccgtac tcgctattta ggcttaccac atgggtggtt ttgccctggg tttgctgcag
     4741 ataaatacct tgggtggtga gttgtggtgt cgccgcttgc tcgctgatga gctcggcctt
     4801 gctgtcgcta ccggccttaa atatacgcat accctgttgg taattggccg cccaatacca
     4861 atactggccg tcgtatttgg ccacatctgc ttcatccaca ccggcgatat gcacattggt
     4921 ggcggaaaaa ttagtgctct cagggctgct gacgggaacc ggattgcccg tggcgccgcc
     4981 agcggtaacg gagtctctta ggtaggtgtt actttggttg agttgcagac gcaggccatt
     5041 ttttaagtgg gcctctgtgg tggttttgct gggggtgtcc aggcgagtgt ccgattgggt
     5101 ggtggcttta agttggttgt agtccacctt ggtctgctcg gtgggtttgc cgcctgagca
     5161 gcctaacagc gcacagctga tactcagccc taaaaggtaa gtcttcatga taatctccca
     5221 aatgcgagtt gttgcgggtg tcttttctac ttttaatcta ctgctaagtt ctaagggtgc
     5281 tacttctgcg gattctgctg tgagtgcagg ccaagggccc gcgcggtaag ccacccaact
     5341 ggcgacaagg ggccgcccgc tgcatgtttg gctgctggca tactagaaag gctcagttgt
     5401 aaaaactgtt aacaaattgt aacggcgggt ggttttgcgg ggtggttcac tgtttggccc
     5461 cgcgcgccgt tttgctgcgg gaatctatgc gaattattga tatgtatgtc tgccgcctgg
     5521 cggaatttag cgacctggat ggtgaggttg cggcgcagtt tttggatgtg caggaacatg
     5581 cgcgcttttc gcggtttaag tttgaccggc atcgccaggc atttttagcc agccgggtac
     5641 tgcaaaaaac cgctttagcg caaaggctaa aatgccccat tgcagatctg gccttcgatt
     5701 ttggccccca gggcaagcct gcattgcagc ggggcattaa ccccggcggc gtggaattta
     5761 atttaaccca cagcgatacc tgggcgctgt tggcggtgga tcaatacccg ctgggtatcg
     5821 acacggaaaa tatgcaacgc aataccaata ttctcagtat tgcccggcat aactttcacc
     5881 ctcgagagat tgcattttta acggcacaag ccgctgagcc tcaatggggt ttttattatt
     5941 ggatgttaaa agaggcctac ataaaatact taggcacagg gcttagccaa tccttaaaag
     6001 aattttattt tacctggcaa cccttgcagt tcggcagcga aacccctctg ccagtgcctg
     6061 cggcctgcgt atttaactgg gcggccgatg cagttgccgc cctgtgttat cagccgggtg
     6121 aagtacacct gcgcctccag cagttatcgc cgcagggcac ctggacacca ctggaaatac
     6181 agctgctagt gcagacccaa tagggtttgg ccgcctgtat ttgcaattat tttgccggta
     6241 tttttggcag cgatacggct tatttaatag ctgcttttga atatttccag agcgccacaa
     6301 ttattctggc gtgcagaatt aacccttggg cgcctgcaaa attttgctgc tgctaattct
     6361 ttggcgcatc tgttgggtgt acgcagagag attttccgcc gcagcgaata atgccgggcc
     6421 atttggtagg gctaataaat aatccagcat gggcagtagg agtaaatcgg caaggcttag
     6481 ggcgtcgccc acccaaaagt gttgggtgcc cagggcggca cttaataggg ctaggtgttc
     6541 cttcactgca ggggttgcat tgtcgattac atcctggcgc aactgaccat tttccccttt
     6601 cggaaatacc cattccaaca gatagtggcg cacaaaaata agatcgcttt gttgattaaa
     6661 tacggcgcac cattggtcga tcagggcgcg ctcacggggc gttttccctt gcaggccagg
     6721 tgcgggaaac tgcgcgtcta aataccgcaa aatactttgt gtttcgctga ggacaaaatc
     6781 atcgtgcact aataccggta ttttccctat agggttaagc gcatttaacg ccgccgactt
     6841 aagcccaatg ggctggccct ggtgctgcat gctaaggctg tagggaatat ttttttcttc
     6901 gcagcataat aaaacgctgc gcacgaggtt gctaaaagga acaccaataa tctgtacact
     6961 catgtctaac tccagtaaac gggctgttgg gtgcgcccta aacgccattc cactaaatgg
     7021 cgcgggtgag ggaaggttaa tgcccggcac catatgttca agaacataag gtgaaaaccg
     7081 gttaagctca ggttttatgg gttacctaat gggtggaatt caatgggttg gagtgcacat
     7141 cacaaaacag cctcgcggga aactattttg ctggccgctg cagaattatt tgcccgttac
     7201 ggatacgacg cggtggctat agaccgggtg atggcccagg cgggtatgac gcgaggagcg
     7261 ttttacgccc acttcacgtc taaaagcgat ttatacgccc aggctatgtt gctggcgggg
     7321 cagcgcttgg tggcgcatag cctgcatctg gggatgaatt ttgaagaatt tgtctgcacc
     7381 tatttaacgc cagggcagct ggccatggcg cccctggcat gcccactggc ctgtttggtg
     7441 accgatgtgg cgccgcgtga agagcgtgtc aaaatagttt acgggcagtt gctggcgggt
     7501 tttgtcagcc atttgcagcg tttaaatccg cgcttaactc aggctcgggc ggcggcttgc
     7561 gccagcagtt taatcggggc tcaggttgtc gcccgtgcgg ttcatacgca ggaattagct
     7621 aaaaatatat tgttgtcggc gcagcacacc attcttgagc attttgcgga taacaaaaat
     7681 gaagcctaat aacgcgttag aaatctgtcg ataaacgaca tatggagaat tattcaggct
     7741 gggtgagggc aaagccttgt tgcaattctg ccgagggggt ggtttttttg tgcagtacgt
     7801 tggttacccc taaaccgctt attaacacgc ctacaacaaa aatgattgct gcaagggata
     7861 atacgtcagt tcgcatcgct ggtagcctcg caattggtga atatgaacgc tgggtaaacg
     7921 tgtgcgcttt atacagtttt ttttcgcact gcacaatcgg ctgtgcggaa atttctgcaa
     7981 gaaaaataaa cgcttgctca caatgagcaa aaaatccgcc ccctgggtta ggtggggtag
     8041 ggggcgctgg tgaggtgggg gcgctttagg ccaaaaataa tctataggcc gggttctggc
     8101 tttcatccca aaacggataa cccaattcct ccaaggcggt aattaaggct tggcgcgaat
     8161 cggcaggcac ctgcatgccc accagcacac ggccaaacgc ggcaccgtgg ttgcggtagt
     8221 gaaacagtga aatattccag cggccggcta atttagtcag aaattgcagc agcgcgccgg
     8281 ggcgctcagg aaattcaaag cgcatcaata attcatcgct caaatcaccc ggcgcccgcc
     8341 cgcccaccat gtggcgtatg tgcagcttgg ccatttcatt gtcggaaaga tccgtcactt
     8401 catagccctg ttggcgcagc cgctggatta aatcctggcg atcgccgcgc tccggggaaa
     8461 tttgtaaacc gacaaaaata tgggcgtttt cctgcgcgtt ttgaaagcgg taattaaatt
     8521 cggtgatcgc gcgcctgccc agcagggtgc aaaactccaa ataactgccg gggcgttcag
     8581 gaatggtgac ggccaaaatc gcttcgcgat tttcgctaat ttccgtgcgc tcagagatat
     8641 accgcaaacg gtcaaaattg gtgttggcgc cggagtctat ggcgataaaa ctctgcccgc
     8701 tcaccccttc gcgggcgacg tattttttta acccggcgag agcggcggct ccggcaggct
     8761 cggtaatcga gcggttatct tcaaaaatat cttttatcgc tgcgcaaatt tcatcggagc
     8821 tgacggtaat gacgccatca atacactcac gcagcacgcg aaaggtctcc tcgccgattt
     8881 gcgccacggc aataccatcg gcaaataaac ccacttgaga cagtacgctg cgctcgccgc
     8941 tggccatggc cgcagctagg cacgccgaat cttccgattc caccgcaaat acttttacat
     9001 cgctgcgcag atatttaatg taagcggcca tgcccgcaca caaaccgccg ccacctaccg
     9061 ggataaacac tgcatccagc gggtgggggt gctggcgcag aatctccatg gccactgtgc
     9121 cctggcctgc gattacatcc gggtcatcaa acgggtggat gtacaccatg ccctgttgct
     9181 gcaccagttg tttggcaaaa gcggccgcct cgtcatagct gtcgccctgt aattcaacgg
     9241 ttgcaccccg gcgtttaacg gcatttacct taatcgcggg ggtagtgcgc ggcatgacga
     9301 tggtggcttt aacgcctagg tgggctgcgg ctagcgccaa gccctgggcg tggttgccag
     9361 ccgacgcggc caccacgcct ttggccaatt gctccggtgt taattgcaat aatttgttgt
     9421 aggcgccgcg aattttgaag gaaaacaccg gctgcaggtc ttcgcgtttg agccatacct
     9481 gattctggca gcgctcagag atgagcggcg caaattccag gggggtctct acggctagat
     9541 cgtagatgcg ggcatttaaa atacgcttga tgtaggactg gggcatagtc aaactggcaa
     9601 aagccgccat ggtaacgcga ttgaccgcca aggtgccaaa cctgcggcga aaaaccttat
     9661 ttgcaaccgt accgtgtccc cgcctggggt gctgttccca gcctaagcgc ttcccattca
     9721 accacctttg gagtgcccat gcttctttcc tctccccttg tcaacgtcgc ttggctgagc
     9781 gaacaactcg atgccgcgaa tttggtgctc ctagatgcca gtatggcgcc gccgggccag
     9841 cccacctatg cgcctgtgca ggtgattcca ggtgcgcgca ggtttgattt tgataaacag
     9901 gtgtgtgatc agaacagtgc tctgccccat atgatgcccg cggctgagca gttcactgag
     9961 gctgcgcagg ctttaggcat caattcagat tcaaccatcg tggtgtacga ccacctgggc
    10021 ttgttcgcca gcccaagggc ctggtggatg ttcaaaagca tggggtttga tgctgtgtat
    10081 gtcctcgacg gtggtttacc cgcttgggtg ctagccgggc tgccgaccct tgagcgctac
    10141 agcccggcgc ttacgcctgg gaatttcacc gcccgttata gcgccgatta ttttgttgat
    10201 aaaacccagg tgttcgcggc gctggataac cccgccatct ccatcctcga tgcccgtccg
    10261 agcgggcgtt ttgccggcac ccaagcggag ccgcgccctg ggctgcgttc aggccatatg
    10321 ccgggggcgt tttccctgcc gtttaccgcc gtgcaaagcg gtgggcagtt gctcggggcg
    10381 caacagttgg ctgagctctt aggtgccccc cggccgctga tctgcagttg cggctccggc
    10441 gtgaccgcct gcgtactggc tctggctgcg gtggtggcag gctttaggga ggtggcggtg
    10501 tacgatggca gctggagtga gtggggcgac ccccaatggc agctgccggt ggttactgaa
    10561 tccgcttgac cacaggatcg ctgggtttat gggcgcgcag tttcatctat gctgtttgct
    10621 taagtaagcc ttagccttac ggcgcgggca gtgattattt tgttaagggg aagacagtga
    10681 aaaaatttca cctggccttg ggcgtggcgg atatagccgc cagcgtcgaa gactattccg
    10741 agcgtttgca gtcccagccc gcccttgtgg tgccgggcga atacgccctg tggcgcacgg
    10801 cccatctgaa tttttcggtg cgcaaagttg ctgctgagca ggtggggcaa ttgcgccatc
    10861 ttggctggga ggaccctcac gcggaggcct tcagctgtag cgaagattgc aatggtgtgg
    10921 gttgggagca ctttaatctg cagcaacaat tagacgaaat cgcgctggct tggcccggct
    10981 cggtcaaggg ctcggggcgt gtttgagctt cggcgcgctg gccatcaagg tgatggtccg
    11041 tgcggttgac tccagcgcgg ggcggcacga cactgcgagc ctttttagga aacctgctat
    11101 gacgttaatt ctcggtatag atccgggctc gcgcaaaacg ggctatgggc tgatactgca
    11161 gcagggccag cggctcaact attgcgccag tggcgttatt cgtccaccgg aagatgcgcc
    11221 cctaagtgag cggctgaaag taattttcga cggtatctcg ctgcttatcg cccagcacag
    11281 ccccgcccag ttcgccatcg agcaaatatt tatg
//
LOCUS       DAOQGS010000054        11287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2657_length_11287_cov_5.118412, whole genome shotgun sequence.
ACCESSION   DAOQGS010000054 DAOQGS010000000
VERSION     DAOQGS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2657_length_11287_cov_5.118412"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="atpA"
                     /locus_tag="PKZ52_03380"
     CDS             <1..1172
                     /gene="atpA"
                     /locus_tag="PKZ52_03380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNG58841.1"
                     /translation="LTDAIEKIAPGVIARQSVDQPVQIGLKAVDAMVPIGRGQRELII
                     GDRQTGKTALAVDAIINQKGSGIKCIYVAVGQKASSVASVVRKLEEHGAMSYTTVVAA
                     TASDPAAMQFLAPFAGCTMGEYYRDRGQDALIIYDDLTKQAWAYRQISLLLRRPPGRE
                     AYPGDVFYLHSRLLERAARVNADYVEAFTKGEVKGQTGSLTALPVIETQAGDVSAFVP
                     TNVISITDGQIFLESNLFNAGIRPAMNAGISVSRVGGAAQTKIIKKLSGGIRTALAQY
                     RELAAFSQFASDLDEATKAQLEHGQRVTELMKQKQYAPQSIAQMGVLLYAANEGYLKD
                     IEVAKLGSFESSLLAYMQNNHAELMDGIVATGNYNDDIEKSIKAALTNFKATQTW"
     gene            1200..1490
                     /locus_tag="PKZ52_03385"
     CDS             1200..1490
                     /locus_tag="PKZ52_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58842.1"
                     /translation="MKACSVIFLSMVAISASAEEASNLTITNFGVQGAGGGYFRVAQP
                     LVPACLFDVIYIANTNYLSAVLAAKMAGKKLSRVAYSKEANGTCNASLIEIE"
     gene            1592..2452
                     /gene="atpG"
                     /locus_tag="PKZ52_03390"
     CDS             1592..2452
                     /gene="atpG"
                     /locus_tag="PKZ52_03390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNG58843.1"
                     /translation="MASGKEIRTQIGSIKNTQKITKAMEMVAASKMRKAQDRMEKGKP
                     YASRMRAVIGHLAHACSEYKHPYLQARSVKRVGYIVISSDRGLCGGLNINVFKRVINH
                     SRPLAASNIGVDMCLIGAKAGAFFKSIGGKILASVRDIGEEPSLTKLIGSVKVMLDAF
                     EEGKIDQLFLVSNDFVNTMTQQPNVMQLLPLMPDDMEQSNHKWDYLYEPEAKILLDGL
                     LRRYIESQVYQAVVENAACEQAARMVAMKNATENAGEIIDELKLAYNKARQAAITQEL
                     SEIVAGAAAV"
     gene            2506..3906
                     /gene="atpD"
                     /locus_tag="PKZ52_03395"
     CDS             2506..3906
                     /gene="atpD"
                     /locus_tag="PKZ52_03395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNG58844.1"
                     /translation="MSSGRIVQIIGAVIDVEFPRDAVPQVYDALTVSGKGLTLEVQQQ
                     LGDGVVRAIAMGSSEGLSRGLTVINSNNPINVPVGIETLGRIMDVLGNPIDECGPIGE
                     QARASIHRKAPAYDELSASEELLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIATEHSGLSVFAGVGERTREGNDFYHEMQEAGVVNVEEFAKSKVAMVYGQMNEP
                     PGNRLRVALTGLTMAEKFRDEGKDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPT
                     LAEEMGVLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIAAK
                     GIYPAIDPLDSTSRQLDPLIIGQDHYDVARGVQSVLQRYKELKDIIAILGMDELSEAD
                     KLTVNRARKIERFLSQPFHVAEVFTGSPGKYVSLKDTIAGFKGLLAGEYDHLPEQAFY
                     MVGSIDEAVEKAAKFK"
     gene            3933..4355
                     /locus_tag="PKZ52_03400"
     CDS             3933..4355
                     /locus_tag="PKZ52_03400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNG58845.1"
                     /translation="MAKAIKCNIVSAENEIYSGEAELLIAATTEGDVGVGFGHSQLLA
                     ALKPGPVRVLKGGDEEIYYVSGGFLEVQPSVVTILADTALRADDMDEAAALEAQKMAQ
                     QAMHNQSGELDYSRAAVQLAEAAAQLRTLQAIRKKLNK"
     gene            4426..7170
                     /locus_tag="PKZ52_03405"
     CDS             4426..7170
                     /locus_tag="PKZ52_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58846.1"
                     /translation="MKRIQLGSVACFLTCALSAPSVFAFKLKTHMWVGQQVINDLEDN
                     GRIDIQLRERQISLPVREEIKAAILNNKQAYLMGNIGPDASPDVIVGQTVVHPGVPGG
                     WKTNDWLKHLLVSSTTNPTAKAYTYGYLGHASADVFAHTYVNQYSGDIFELFDGETLV
                     EKRHVMLEGFIEKYTPPLTDYRGNALGPYYSPQNVQVNSALATFLRNALIYDPNVQYQ
                     YKLSVGASHLAGVARYRNRIQEAANSEVWKSLDAKIVQLIASYYEIPLSDAEAKQIIA
                     WKEDLAHGADKIGLDNLERKGAAVLADLEHDAANVAIDNLKSQLNALHSAQSKIISLE
                     QQVLDARAKVFTQATEAVACDILDDVLGVDSWKDLILPGYSFISDLFGSDPRPEKIYV
                     NLSGGGAVKYIQDDLKMLQERAVTFQASADYQIEFNGFVTLWAASDIRQAYEARENMR
                     MISLVLSAGLADTDTYEFKTYTNGSEYLSSIEHGLIYSKGSTGSYVKSDDSFCHKVTF
                     VAEKIDGAANQLIIDGQKALLSEQQKAVDAGLSVANELIKINDAKNQILIAAIELKNI
                     FAAGGIVPKNPMQALLQNWVNDIDQQMSLYVQATAKSMINTMDPSTSAAEPLVEWFKC
                     FDSVFLGVPQPICTVRNSIADLNKAVGVVQGILAKTAGPAASELYQLKENLIKAAKGE
                     VEELAKKKINAAIPPELKQLIDILEADVDDQKLNDLYSSLESESVFKSILLMPNVASA
                     VKSDMAIANNQFDPVKFAAIYDAVVLAKLTLLDRAGLVELANTAGAQMRDGTNPFANV
                     DNIISDAVANIDGNHQWMDYSPPLAKMHGPFTNMPVYSTAAGFVPWKADVRDALFRRL
                     FKGPISKEIDGMGAAFVSGIIASDYPYHTCAANPFPGSEKDQLCIVIPLLPILGLLLN
                     "
     gene            7322..7666
                     /locus_tag="PKZ52_03410"
     CDS             7322..7666
                     /locus_tag="PKZ52_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58847.1"
                     /translation="MLKKILLGLVFFVASTAHAGSWITSDGTAKITKIQTEGGLTVVW
                     FDSIDRWVSADGCTVQGKAILQDDTKNGDRQYAALLAAFATGKPIMMFGSGCVSAWGT
                     TFPKIWSVISVM"
     gene            8026..8598
                     /locus_tag="PKZ52_03415"
     CDS             8026..8598
                     /locus_tag="PKZ52_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNG58848.1"
                     /translation="MNEQHTNMATNLFSRLIFSSRWLQLPLYFGLIIAQCAYVYLFVH
                     ELWVLVSTVSTLGEQQIMLIVLGLIDVVMISNLLVMVIVGGYETFVSRLRLDGHPDQP
                     EWLSHVNATVLKVKLAMAIIGISSIHLLKTFIEAGNIGMAGAKFTEAGVMWQTIIHMA
                     FIVSAIGIAYTDWLMQKVSSSAEASHHHVQ"
     gene            8921..9439
                     /locus_tag="PKZ52_03420"
     CDS             8921..9439
                     /locus_tag="PKZ52_03420"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNG58849.1"
                     /translation="MTEGILWILRSGARWKDLPAHYPSPSTCWRRLQYWEESDSWLKA
                     WRKFLRQLDRKSLLNWEEVFSDDSFASAKKRGPEVGKTKRGKGSKWIVVVDGEGIPLG
                     GTVTSASPAEVTLIEPLLDVTYGKSKIKRLIYDKAADSDPLRKSLSNRNIELVCPHRN
                     NRKKPPTQMAGR"
     gene            complement(9635..10675)
                     /locus_tag="PKZ52_03425"
     CDS             complement(9635..10675)
                     /locus_tag="PKZ52_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011497995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HNG58850.1"
                     /translation="MLQTTNPIIKHKAGLLNLAEELGNVSKACKIMGFSRDTFYRYQE
                     LAQEGGVDALMNKSRRTPNHKNRVDETVEQAVLAHAIAQPAHGQHKTSNELRKLGVFV
                     SGSGVRSIWLRHNLENFKKRLKALEDKVAAEGILLSDEQIAALERKKMDDEVCGEIET
                     HHPGYLGSQDTFYVGNLKGVGRIYQQTFVDTYCKIAFAKLYTSKTPITSADLLNDRVL
                     PYFEQHDLPMLRILTDRGTEYCGRMDQHDYQLYLAINGIEHTKTKAHSPQTNGICERF
                     HRTILQEFYQVVFRKKLYSRLDDLQRDLDEWIDYYNEERTHQGKMCCGRTPLATLLEG
                     KHIWAEKNLAQI"
     gene            complement(11028..>11287)
                     /locus_tag="PKZ52_03430"
     CDS             complement(11028..>11287)
                     /locus_tag="PKZ52_03430"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNG58851.1"
                     /translation="YHERISAALDCAFYFAHPYSSWQRNSNESTSGMLRQYYPKNTDF
                     KLVDDEHLRRVINELNKRPRKVLGFRTPMDLMNNQLAAPAA"
BASE COUNT         2980 a         2415 c         2799 g         3093 t
ORIGIN      
        1 aattaaccga cgcgattgaa aaaattgccc cgggtgtaat tgcccgtcaa tcggttgacc
       61 agccggtgca aatcggtctt aaagccgttg atgccatggt tccaatcgga cgcggtcagc
      121 gcgaattaat tatcggcgac cgtcaaaccg gtaaaacagc ccttgcggtc gatgccatca
      181 tcaaccaaaa aggctccgga attaaatgta tttatgtggc cgtaggccaa aaggcctctt
      241 ctgttgcgtc ggttgtgcgc aaactcgaag agcacggcgc tatgtcttac accactgtag
      301 ttgcggctac tgcttctgac cctgcagcta tgcaattttt ggcacctttt gctggttgca
      361 ccatgggtga gtactaccgc gatcgcggcc aagacgcgtt aattatttat gatgatttaa
      421 ccaagcaggc ttgggcctat cgtcaaatct ccttgttatt gcgccgccca cccggtcgtg
      481 aagcctaccc aggggacgta ttctatttgc actcacgttt attagagcgc gcagcaagag
      541 taaatgccga ttacgtagaa gccttcacta agggtgaagt taaaggccaa accggttctt
      601 taactgctct gccagtaatt gaaactcaag ccggtgacgt atctgcattc gtacctacca
      661 acgtaatttc cattaccgat gggcagatct tcctggaatc taacctgttt aacgcaggta
      721 ttcgccctgc gatgaacgcc ggtatttcgg tatcccgtgt gggtggtgct gcgcaaacta
      781 aaatcatcaa gaaactctcc ggtggcattc gtaccgctct agcgcagtac cgtgaattag
      841 cggcgttttc tcagtttgct tcggatttgg acgaagccac taaagcgcag cttgaacacg
      901 gtcagcgcgt aactgaattg atgaaacaaa aacaatatgc accgcagtcc atcgcgcaga
      961 tgggtgtgtt gttgtacgcc gctaacgaag gttatttaaa agatatcgaa gttgccaaac
     1021 tcgggtcttt tgagtcctcg ctgcttgcct atatgcaaaa taaccatgcc gaacttatgg
     1081 acggcatagt ggctactggc aactacaacg atgacattga gaagagcatt aaagctgctt
     1141 taaccaattt caaagccacc caaacctggt aatgacacgg aacggaatag gttgacccaa
     1201 tgaaagcatg tagtgttatt tttctgtcga tggttgcgat ttcagcgagc gccgaagaag
     1261 cttcgaattt aactatcacg aacttcggag ttcagggggc agggggtggg tattttcgag
     1321 ttgctcaacc tctagttcca gcttgtttat ttgacgtaat ctacattgca aacaccaatt
     1381 atttgtctgc agttttagcg gcaaaaatgg cgggtaaaaa gctttcaagg gttgcctatt
     1441 ccaaagaggc gaatggtacc tgcaatgcct cgctcataga aatcgagtga gtgatgacag
     1501 tactcaattc gcttcggtgt gttggtgtgc tgtttagggt tgacggacag taaacggtat
     1561 gtcgtttaag atacgcacag cgagataaac aatggcaagc ggaaaagaga tacgcaccca
     1621 gatcgggagt attaaaaata cgcagaaaat caccaaagcc atggaaatgg tggcggcgag
     1681 taaaatgcgt aaggcgcagg atcggatgga aaagggcaaa ccctacgcca gccgtatgcg
     1741 cgcggtaatt ggccatttgg cccacgcctg ttctgaatac aaacaccctt atttacaagc
     1801 gcgctcagtt aagcgggtcg gttacattgt tatttcctcc gaccgcggtt tgtgcggcgg
     1861 tttaaatatc aacgtattta agcgtgtgat caaccacagc cgtccattgg ctgccagtaa
     1921 tatcggcgtg gatatgtgtt taatcggcgc taaagccggt gcgtttttca aaagcatcgg
     1981 cggtaaaatt ctggcctctg tgcgcgatat aggtgaagag ccttcgctga ccaaattaat
     2041 tggcagcgta aaagtaatgc tcgacgcttt tgaagaaggc aagatcgacc aattattttt
     2101 ggtgagcaac gactttgtca acaccatgac ccagcaaccc aatgttatgc agttattgcc
     2161 attgatgccg gatgatatgg agcagagtaa tcacaagtgg gattatttgt acgagccgga
     2221 agcaaaaata ctgctggatg gtttattgcg ccgttatatc gaatctcaag tgtaccaagc
     2281 cgtggttgaa aacgccgcct gtgaacaggc agcgcgtatg gtggcgatga agaatgccac
     2341 cgaaaacgcc ggcgaaatta tcgacgaatt aaaacttgcg tataacaagg cgcgacaagc
     2401 ggcaattacc caagagcttt cggaaattgt tgccggtgct gccgcggttt aattcgcgtc
     2461 ggcctttaac gaattatttt ttatttttag gtagaggaaa ctcacatgag tagcggacgt
     2521 atcgtgcaaa ttatcggcgc ggtaatcgac gtggaatttc cacgcgatgc tgtaccccag
     2581 gtttacgacg ctttaacggt aagcggtaaa ggcctgaccc tcgaagtaca acaacagttg
     2641 ggtgatggcg tagtgcgcgc aattgccatg ggttcatccg aaggcctaag ccgcggatta
     2701 accgtaatta acagcaacaa ccctatcaat gtacccgtag gtattgaaac cctcggccgc
     2761 attatggacg tgttgggcaa ccccatcgat gagtgcggcc ccatcggcga acaagcgcgc
     2821 gcgtctattc accgcaaagc acctgcttat gatgaattaa gcgcgtcaga agaattgctt
     2881 gaaaccggta ttaaagttat cgacctggta tgcccatttg ctaagggtgg taaggttgga
     2941 ctgttcggtg gtgccggtgt aggtaaaact gtaaacatga tggaattaat taacaacatc
     3001 gccaccgagc actcaggttt atcggtattt gccggtgtgg gtgagcgtac tcgtgaagga
     3061 aatgacttct accacgagat gcaagaagcc ggcgttgtta acgttgaaga atttgccaaa
     3121 tctaaagtgg cgatggtgta cggccagatg aacgagccac cgggcaaccg tttgcgcgtg
     3181 gccttaaccg gtttgaccat ggcggagaaa ttccgcgacg aaggcaaaga cgtattgttg
     3241 ttcgtggaca atatttaccg ttataccctg gccggtaccg aagtgtccgc actgttaggc
     3301 cgtatgcctt ctgcggtggg ttaccagccg actctggcgg aagaaatggg tgtattacaa
     3361 gagcgtatta cttccactaa aaccggttca attacctcta tccaagcggt atacgtacct
     3421 gcggatgact taactgaccc gtcgcctgcc accaccttcg cgcacttgga ctccacagta
     3481 gtattaagcc gtgatatcgc tgctaaaggt atttacccgg cgattgaccc actggattcc
     3541 acttcgcgtc agctggaccc attaattatc ggtcaagatc actacgacgt ggcccgtggc
     3601 gtacagtcag tattacagcg ctacaaagaa ttaaaagaca tcatcgccat tttgggtatg
     3661 gatgaattat ccgaagcgga taaactcacc gtaaaccgcg cccgtaaaat tgagcgtttc
     3721 ttgtcgcagc ctttccacgt agcggaagta tttaccggct cacccggaaa atacgtgtca
     3781 ttaaaagaca ccattgcggg ctttaaaggt ttgctggccg gtgaatacga ccacttgccc
     3841 gagcaggcgt tttacatggt cggctccatc gacgaagccg ttgaaaaagc cgctaaattt
     3901 aaataatcgc tatttagcgt gaggaattga gcatggctaa agcgattaaa tgcaacatcg
     3961 tcagcgccga gaatgaaatt tattccggcg aagccgagtt gttaattgcc gctaccaccg
     4021 aaggcgacgt aggtgttggc ttcgggcact cacaattatt ggcggcctta aaacccggcc
     4081 cagtgcgtgt tttaaaaggc ggtgacgaag aaatttatta cgtgagcggc ggttttttag
     4141 aagtgcagcc aagcgtagtg actattctgg ccgatactgc cctgcgcgcc gacgatatgg
     4201 atgaagccgc tgcgttagaa gcgcaaaaaa tggcgcaaca ggcgatgcac aatcaaagcg
     4261 gggagttgga ttattcccgt gcggcggtgc agttggctga agcagccgcg caattgcgta
     4321 cactgcaggc aattcgcaaa aaattaaaca aatagaattt ttatttgtgt tcaataagca
     4381 gcttaggctg cttttttttt aaaatatatc taggaagaat ccagaatgaa acgtattcaa
     4441 ttgggatcag ttgcttgttt tctaacctgt gctctgagcg caccctctgt atttgctttt
     4501 aaactcaaaa cccatatgtg ggttggacag caagttatca acgatttaga agataatggc
     4561 agaattgata ttcagctgcg ggagcgtcaa atatctttgc ccgtgcgcga ggaaattaaa
     4621 gcggcgatat taaataataa acaggcctat ttgatgggca atatcggccc tgatgcatca
     4681 ccggatgtta ttgtcgggca aaccgttgtg catcctggtg tgcctggcgg ctggaaaact
     4741 aatgactggt taaaacattt actggtcagc tccaccacaa atcccacggc aaaggcttat
     4801 acatatgggt atttggggca tgcctcggcg gatgtttttg cgcatactta tgtgaatcag
     4861 tattcaggcg atatatttga attgtttgat ggtgaaactc tagttgaaaa acgccacgta
     4921 atgcttgagg ggtttatcga aaaatataca ccacctctaa ccgattatcg cggcaatgcg
     4981 ttaggacctt attatagtcc acaaaatgta caggtgaatt ctgcgctggc gacattcttg
     5041 cgcaatgcat tgatttatga tcccaatgtt caataccaat ataaactctc agtaggtgct
     5101 tcccacttgg cgggtgttgc gcgttacagg aatcgcattc aagaagccgc caatagtgag
     5161 gtgtggaaat ccttggacgc taaaatagtc caacttattg ccagttatta cgaaattccc
     5221 cttagcgatg cggaagcaaa acaaattatt gcgtggaaag aagatttggc ccatggtgcc
     5281 gacaaaatag gcttggataa tttggagcgc aagggggctg cggtattagc tgacttggag
     5341 catgatgcgg cgaatgttgc tattgataat ttgaaaagcc aattaaatgc actgcacagt
     5401 gcacagagta aaattatctc gcttgagcag caggttttgg atgcgcgcgc taaagttttt
     5461 acccaagcaa ctgaggcagt agcttgtgat attttagatg atgttttggg ggtggattcc
     5521 tggaaggatc tgattttgcc aggatattct tttatatctg atctgtttgg ttccgaccct
     5581 cgacccgaaa agatatatgt aaaccttagc ggaggcgggg cggttaaata tattcaggac
     5641 gatctaaaaa tgttgcagga acgagctgtt acctttcagg ccagtgcaga ctaccaaatt
     5701 gagtttaatg gatttgtgac actctgggcg gcttcagaca tcagacaagc gtatgaagct
     5761 agagagaata tgagaatgat ctcattggtg ttaagtgcag gattggcgga tacagataca
     5821 tatgagttca aaacttatac caatggcagc gagtacttaa gctccattga gcatggcttg
     5881 atttattcaa aaggtagtac aggtagctac gtaaaatcgg atgattcctt ttgtcacaag
     5941 gtcacgtttg tcgctgaaaa aatagatggc gctgccaacc aattaatcat tgatgggcag
     6001 aaggcgcttt tgtccgaaca gcaaaaagcg gtggatgcgg gactgagtgt agcaaatgaa
     6061 ctaattaaaa ttaatgatgc caaaaatcaa attctcattg ctgccataga attaaaaaat
     6121 atctttgccg cgggcggcat agtgccaaag aatcccatgc aggcattatt gcaaaactgg
     6181 gtcaatgata tagatcagca gatgagttta tatgttcagg ctacagcaaa aagcatgatc
     6241 aatacgatgg acccatccac gtccgccgca gagccgctag ttgagtggtt taaatgtttt
     6301 gattcggtat ttctaggtgt gccgcagccg atttgtactg tgcgaaatag catagcggat
     6361 ctaaataaag cggtcggcgt ggttcaggga attcttgcta aaactgcggg tccggcggcc
     6421 agtgagttgt accagctcaa ggaaaacctg attaaagcgg cgaaaggaga agttgaagaa
     6481 ttagctaaga agaaaatcaa cgcggctatt ccgcccgagc taaaacagct gattgatata
     6541 ttagaagcag atgtcgatga tcaaaaactg aatgacctct attcgtcgct ggaatctgaa
     6601 agtgtattca aaagtatatt gctgatgccc aatgtcgcca gcgctgtaaa aagtgatatg
     6661 gctatagcca ataatcagtt cgacccggtg aaattcgcag cgatttatga tgctgtcgtt
     6721 ttggctaagt tgactttatt ggatcgcgca ggattggttg agcttgcaaa tacggcaggt
     6781 gcgcaaatgc gcgatggcac taatccgttt gccaatgttg acaatattat ttctgatgcg
     6841 gttgctaata tagacggaaa tcaccaatgg atggactact cgccgccact agctaaaatg
     6901 catggtccgt ttacgaatat gcccgtgtac tctacggcgg caggatttgt gccttggaag
     6961 gcggatgtgc gggatgcgtt gttccgaagg ctctttaaag ggccgattag caaggaaatc
     7021 gatggtatgg gggcagcatt tgtaagtggc atcattgcct cagactaccc atatcacacg
     7081 tgcgcagcca atccatttcc tggaagtgaa aaggatcaat tgtgcatagt aattccgctt
     7141 ttgcctattt tggggttgtt acttaattag tagtggataa gaaaataatt gagcacagta
     7201 tttgagcgca tcggtatttc tgtcccattg attttgaagt gcgcctgctc atcgatttgg
     7261 gcatttcact attgcggccc cctgttttgg ggctgtggtt tttcattcag gagagtttta
     7321 tatgttaaaa aaaatattgc taggattggt tttttttgtg gcttcaacgg cgcacgcagg
     7381 tagctggatt acatccgacg gcacagcaaa aatcacaaaa atccagacgg aaggtggatt
     7441 aaccgtggtt tggtttgact ctattgatag gtgggtttca gcagatggtt gtaccgttca
     7501 agggaaagct attttgcagg acgatactaa aaatggtgac cgtcaatacg cagcactctt
     7561 agcagcgttt gctacaggca aaccaattat gatgtttggg tccggatgtg tttccgcgtg
     7621 gggtactaca ttccctaaaa tctggagtgt tatttctgta atgtagtggt tatttgctta
     7681 tgaataattt ctaatggcgg aaaattgttt tctagttcgg atttcatctc gcgttttaaa
     7741 ctgaaacgtt gcaacactgt tgtaacctgt gagctttgta tcgtcgcagc acattgtgat
     7801 taaaggcact tgtacatagc cgctgggcat ttttgggttc aatttatcta aggcgctgaa
     7861 aaaccttcgt ttaggttgcg cgccgcctca actaggtgtt gccagcttac ctatcagggc
     7921 acatctgtca cccgccgcaa tttgtcggtg cgtgaggcag tgttttacct gcggacattt
     7981 tgaggataaa agctagtttt ccaaatgatt taactggatt gagttatgaa tgaacaacat
     8041 acaaatatgg caaccaacct tttttcacgg ttgattttta gttcccgctg gctgcagttg
     8101 ccgctttatt ttgggttgat tattgcccag tgtgcttatg tatacctgtt tgtgcatgag
     8161 ttgtgggtat tggttagcac ggtttccacc ctgggtgagc agcagattat gctgattgtg
     8221 ctggggttga ttgatgtggt gatgatttcc aacctattgg tgatggtaat tgtcggcggc
     8281 tatgaaactt ttgtctcgcg cttgcgctta gatggtcatc cggatcagcc cgagtggctc
     8341 agccatgtaa atgccacggt tctcaaagta aaattggcca tggccataat cggcatttcc
     8401 tcaattcatt tattaaaaac ctttattgaa gcgggtaata ttggcatggc gggggctaaa
     8461 tttactgaag cgggtgtcat gtggcagacg attattcata tggcatttat tgtctcggca
     8521 attggcattg catataccga ttggttgatg caaaaagtca gctccagtgc cgaggcctct
     8581 catcaccatg tacagtaact gttaatttaa gggtttgatg gtgctttgcc actcctttta
     8641 gctccgtcct ggtttcattt ctagcgcggt tgtaaatgga gaggttgatt ttactcgatc
     8701 cccctttctt atttttgaca atacctgtgg aaatttagtt ttcgcatttt tgcctagaag
     8761 ctgtttcata acttgaaatc tagtaccctg gccgtttctt gtcgagatat ggtagtgggt
     8821 atgagtcgat tcgcgtcaga attaacagac acacagtgga aacaaattga accttgctta
     8881 ccgccgttaa agcgagggcg aggagggccg aagcctatcg ataaccgaag gcattttatg
     8941 gattttgcgc tctggggcgc gctggaaaga cctgcccgca cattatccat ccccaagtac
     9001 gtgttggcgc aggttgcagt attgggaaga gagcgattct tggttaaaag catggcgaaa
     9061 gtttttaaga caattggacc gaaagtcgtt attaaattgg gaggaagtgt tttcagacga
     9121 cagctttgct tctgcaaaaa aaaggggccc cgaagttggc aaaacaaagc gcggcaaggg
     9181 ttcaaagtgg atagtggtgg tcgacggcga aggtattcct cttggaggca ccgttacctc
     9241 ggcatcacca gcggaggtaa cactcattga gcctctgctt gatgtgacgt atggaaaaag
     9301 caaaataaaa cgacttatat acgataaagc ggcagactca gatccgctgc gaaaaagcct
     9361 gagcaaccgg aatatcgaat tagtttgccc acacagaaac aatcggaaaa agccgcccac
     9421 acagatggca gggcgttaag acgatatgct cggcggtgga aggtcgagcg cacatttgca
     9481 tggctcggca attttcgcag actcgtggtt cgctgggaaa aacatgtgag cgtttatagg
     9541 gtttttttca atattgcatg catgttgatt acattaagtg tattagctaa gacccgatct
     9601 gacagttacc gatttttcaa gggtgcctgt cagtttagat ttgagctaaa tttttctcag
     9661 cccaaatgtg tttgccctcc aataaagtgg ctaaaggtgt tcgcccgcag cacattttac
     9721 cttgatgggt tcgctcttca ttataataat cgatccattc atctaaatcc ctctgtaaat
     9781 catccaatct gctatagagt tttttgcgga agacaacctg gtaaaactct tgcaaaatgg
     9841 ttctatgaaa tcgctcgcag atcccattgg tttgcggcga gtgagccttg gttttggtgt
     9901 gttcaatgcc attaatggcc aaatacagct gataatcatg ctgatccatg cgcccacaat
     9961 attccgtccc tcgatccgtc aatatgcgca acattggcaa atcatgctgc tcgaaatacg
    10021 gcaatacacg gtcatttaat aaatcggctg acgtaatcgg cgtcttgctg gtgtagagct
    10081 tcgcaaaagc aattttacaa taggtatcaa caaaggtttg ttggtaaata cgaccaaccc
    10141 ccttgagatt gccaacgtag aaagtgtctt gggaacccag gtaaccagga tggtgcgttt
    10201 cgatttcgcc gcacacttca tcatccattt ttttccgttc cagagctgca atctgctcat
    10261 cgctcaataa aataccctca gcagcaactt tgtcctctaa cgcttttaag cgctttttaa
    10321 aattctctag gttgtgcctc agccagattg agcgcacgcc actgccggag acaaatacgc
    10381 ccaatttgcg cagttcattg ctcgttttgt gctggccgtg ggccggttgc gcaatggcgt
    10441 gcgctagaac ggcctgctca accgtttcat cgacccggtt tttgtgattt ggcgtgcgac
    10501 ggcttttgtt catcagcgca tcaacgccgc cctcttgggc cagctcttgg taccgataaa
    10561 aggtgtcccg tgaaaagccc atgattttac aggcttttga cacattgcct aattcttctg
    10621 ccaaattcag cagcccggct ttatgtttaa tgatgggatt ggtagtctgt agcatgagag
    10681 ttacctttgt gttttggtta ggattcgaca cccatattca aaatcggtaa ctctcttctt
    10741 tcaagccgaa gtgtcagatc aggtcgagac taatacacat taagtaagtt atgaaatagc
    10801 ttctacgttt tttgtgatct gtggtcattt ttgtaaatcg caaaaacaca tttttcgatt
    10861 ttttacccgc ttgtcaattg cccccgcaaa atattcatgc ctgcagtctt agctggagca
    10921 tccttaaggt attgtatttc gagagtattc atcagtattc gttggggcaa tttgcataac
    10981 tagcctggct tttgaccggc ggattcaacc ctgaggtgca tcattcatta agcggccgga
    11041 gccgccagtt ggttattcat taaatccatc ggtgttctaa atcctaaaac cttgcgcggg
    11101 cgcttgttta gctcattaat tacccgtctt aaatgctcgt catcaactaa cttaaagtcg
    11161 gtatttttcg gataatattg ccgcagcatg ccgctggtgc tttcgttcga gttgcgctgc
    11221 caagacgaat acggatgggc aaaataaaat gcacaatcca aggcggctga tatacgttca
    11281 tgatacg
//
LOCUS       DAOQGS010000055        11204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2690_length_11204_cov_4.273657, whole genome shotgun sequence.
ACCESSION   DAOQGS010000055 DAOQGS010000000
VERSION     DAOQGS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_11204_cov_4.273657"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..>3512)
                     /locus_tag="PKZ52_03435"
     CDS             complement(301..>3512)
                     /locus_tag="PKZ52_03435"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HNG58852.1"
                     /translation="SSSSSSSSSSSSSSTSSSSSSSSSSSSSSSSSSSTSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTGAPIVTITAPQSPVIVRGDFKVNAN
                     LTLSPNSKLSSLVVLVNNQAQIISQYQFIAAANKSWQTIQTDIPTGWHLAIINNQEEQ
                     DYLAGELTSRFPNAAANSSFIMGARQNANGGWLWADNTMVNYSAWWFGQPALQSLAVG
                     VMQKDQNWRWSAVATTQTQFIAGYILERESSEIEPSFIASPTAGSAEFVVKQNGLASG
                     SNTLVLQAKDSNGQVGTASLIATNRPNTTPVITQVTPDFSQPQTDAITVQGYVSTYGK
                     TKIATTNWSLTLPTANPVASFKTDASGNFSFDLPSNLLIPTGNLLTIKAVNDANQTGQ
                     GQYTFDFAPTKPSISLALKDGDTLSKAQLSGTLDLPTQQTAKLEISINNGSPQLLNAS
                     QTSGVFTLAFTTAGLKPADNTLLITATSNLGAVTQVQLQKIAVAPPTVTISAPSANQT
                     VNGTVHITGTVNSIFALSTIQLAVDAINQANIINTAQSPNFDFAIPINTLSAGGHTAT
                     VTAVASVAGAAAFSGSQTRAFIYALPPKLNLATPVEGSTVTDFVSVSGNFDLGAQSLK
                     ALDLWINGAAAISITQYITGNQYNYYVSPSQLNDGINSVAVSITDSQGLSSAVKTEFI
                     YKKPSVATLLLTPVNGGSASGDVLVSGKVYSAAALTSATLTTNNQTSKNILSSINNGG
                     DFSVVMKSSEILGNTTPSAQGNSIRLSLNASSTESKSQATSSFTYFQNPNFVPGGDLV
                     VINDLDPFSAYLSSANNREFALNLINYTNPGKPRAAAKQIVFDAEYGTASDNYLSLSK
                     YYQDNNWSVTIFQTETSSAPVTTKFYKDIPSDVKVIFLWEPRAPFTAEKVAALKQFAQ
                     EGGRVVFMGEGPLHYSAIDSVENPLLKQLGTSTLAGKEYSTFSGALTVSATPHQLTTG
                     IKQIRVNVPLELSLGATDFPLLTDADGKVVIAVTRIDGN"
     assembly_gap    3513..3612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3613..>3798)
                     /locus_tag="PKZ52_03440"
     CDS             complement(<3613..>3798)
                     /locus_tag="PKZ52_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58853.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGS
                     SSSSSSSSSSSSSSSSS"
     assembly_gap    3799..3898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3899..6095)
                     /locus_tag="PKZ52_03445"
     CDS             complement(<3899..6095)
                     /locus_tag="PKZ52_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNG58854.1"
                     /translation="MFKSLPSRWLLAPLFALVISGCGEGKSSRVDPAKAQLTPAQAQA
                     REEAERVKAGNDLQVANPTKPSANSSLSVDAGPDIFSDANLLVDLQGSAPSDVSFART
                     RWVQIDGPQASLVNADQLQTQVLVPDVSQYTKLSFRLSASTADQQVASDTLDLFIYPL
                     NNAAKVVSSGQAENTGRVDFTVRLNKPASQVTELNYTTADGTAKAGSDYVPVAAKLVF
                     NPGEQEKIVSVTVIDDKTREKGEYFSLRVIGGTAQAPFNAIGVAVIQDNETGTSPPTS
                     PKPTPSPNADKPQNQLSSQAGQVRVNLVWSDAASRLELAVQDPCAHWIAPNRFEAECL
                     GARGVFEELSSLDPQQNIFWPQVAPVGRYTVRLTHVAGLGSDYTARVYWGENSTQLTG
                     SIGNGDSQDIFSFDVTEADAGTASSSSSSSASSAASSSSSSSSSSSSSSSSSSSSSSS
                     SSTSSSSGSDLVGNMLGNTLVITRSYPTQTTPYTTWNPTSVSTRVTAGTADQIHWNAG
                     PNQTEHALIDPEANEIKFILPEPSVYLADTDVFDGFVLSGFDNPIRSASLIDNTTGME
                     IALTHTAQEVYLNLKGQYPAGTGFTVRIIFDPKVIGSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSASSSSSSSSSSSSSASSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS"
     gene            complement(6754..7545)
                     /locus_tag="PKZ52_03450"
     CDS             complement(6754..7545)
                     /locus_tag="PKZ52_03450"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG58855.1"
                     /translation="MLKIVFADPRFEAYWLTDKQVAIGSGDDNQLILNDPSISPRHAR
                     ILATSDGFVIKDNGSHLGTYVNGEPVSQAKIAAGDQLRLGEIKLTLVDPMASLDGVKS
                     GLRSWSLVACSNFLSGQEFPLPGATDGSTRLTLGRGKDCDLVFPGTHLSRQHLAITLR
                     EDHLELEDLNSANGTFVNETRIHTSKAVPGDKLRLDVYTFKIIGPSPAEAKTQLRAPA
                     RRGDLKPTSPGNRIDPVEPTQSSWPLLASIGFLGVFIALAVYLFV"
     gene            7919..8239
                     /locus_tag="PKZ52_03455"
     CDS             7919..8239
                     /locus_tag="PKZ52_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNG58856.1"
                     /translation="MESQQLPRVVIHYCTLCQWQLRAAWMAQELLSSLSGELFEVALR
                     PGQGGVFEIWVNEHLVWERKRDGGFPGAKELKQRVRDVACPEKILGAHLERGAEPTPS
                     GTQE"
     gene            8256..10814
                     /locus_tag="PKZ52_03460"
     CDS             8256..10814
                     /locus_tag="PKZ52_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNG58857.1"
                     /translation="MAHSHFTHAWVSWVIKNPRWTLLLLVVLTLAAAAGLPRFKLDAS
                     ADSLTLEADKDLDYFRQVSKQFNSGDFLVVTYTPKQDLFSDAALDHLGKLHAELAAIE
                     GVATVNSILNVPLLYSPKQNLREIAKQPRTLLPQDPTQTPTDRALAKHEFLTSPIYRQ
                     LILSPDGQTTALQLNLSVDKTLIDLVSQRDALLTKRHQEGLNQSEEQLLSKVAAEYLQ
                     LRTQADARSHARVAKVREVVGRYKNEAQIFVGGLTMITADMVDYIRSDLMIFGTASFL
                     FMVLILAVLFRNLRYVLIPIASCVMSIVMMLGFLAWIDWRLTVISSNFVALLMIISLT
                     VTIHLVMRYREYVASHPSWSREQLIMATLSFMAMPSWNNTLTTVVAFASFVTSGIRPL
                     IDFGWMMSIGLMVALVLSFVTLPAFLMLLPKDNAAPAVHPLTKNRRSISAWCGVLVEK
                     CGPGIFWGGLLLGALSFWGIAKLEVENRFIDYFHADTEIHQGLSLIDNKLGGTTSLDI
                     IITPKPASSGPGGDADPFADPEKEADPFNNDEDAFADGDPLSENDAKTSVWMTVGGME
                     YVQKIHDYLETLPEVGKVQSLATLYKVGKDINGSLNNFELALMEKSLPEAVSRVLFNP
                     YLDKSTDQTRITLRVKDSYPGLKRAELIERIRNHISALDGMDIQEVRYSGLLVLYNNM
                     LQSLYTSQIATLGWSFVVIFIMFLVLLRSFLLSVVATVPTAISAAVMLGGMGFAGIPL
                     DMMTITIASITVGIGVDNTIHYLHRFEEELQLDGDYLATMHRCHASIGHAIYHTNNII
                     VFGFSIMVLSSFIPTIYFGALTGVAMMVALAGTMFFLPQFILMIKPFKVFKTAA"
     gene            complement(10910..>11204)
                     /locus_tag="PKZ52_03465"
     CDS             complement(10910..>11204)
                     /locus_tag="PKZ52_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNG58858.1"
                     /translation="WESSTADIATRFGYTPERDPYIKGITKERNDQLSARYESLFKLF
                     IKHKDKIDRVTFWGVSDDMTWLNDFPIKGRTNHPLLFDRTHQPKDVYFRLLQL"
BASE COUNT         2524 a         2557 c         3085 g         2838 t
ORIGIN      
        1 ctattcagca tctttttatg tggctggagg taattcctgc actgactggg aactatcgcg
       61 tgcgtgtttt agtgctgtga ccccgtaatc cacagctaca ccgcgaatcg attatgccgg
      121 atggctaagt cctcctgaat ttgccgcccg cgcggaagag gggagtaaag tgtgctgaat
      181 agtttcagtt taataagaga tgttcttaat cagcagagcc tggaggttgc ttcagaccgt
      241 caaaaatagg gttgtttttg acggtctgat aagggcgtat aggcgggttt tggcaatatt
      301 ttaattgccg tcgatgcgag tgacagcaat caccactttt ccgtctgcat cggttagcag
      361 cgggaagtca gtggcgccta ggctcaattc gagcggcaca ttaacgcgta tctgtttaat
      421 acccgtagtg agttggtgag gtgttgctga cacagttaac gcgccggaaa atgtcgaata
      481 ttctttgcct gcgagggtgc tggtacccag ttgttttaag agcgggtttt ctacgctgtc
      541 tatggcggag tagtgcagcg ggccttcacc cataaatact accctgccgc cttcttgggc
      601 aaattgtttt aaagccgcta ctttttcagc ggtgaacggc gctctaggct cccataagaa
      661 gattactttt acgtcgctgg gaatatcttt gtagaatttt gttgtcaccg gcgctgagga
      721 tgtttctgtc tggaagatcg tcacactcca attattgtcc tgataatact tagacagcga
      781 taggtaatta tcgctagctg tgccgtattc cgcatcaaat acaatctgtt tagcggcagc
      841 acggggcttg ccggggttgg tgtaattaat gaggttgagg gcaaattcac ggttgttggc
      901 cgaggataaa taggcactaa agggatctaa gtcgttaatt accaccaggt caccgcccgg
      961 tacaaaatta gggttttgaa aataggtaaa ggagcttgtc gcctggcttt tactctcggt
     1021 ggaggacgca tttaagctta ggcgaatgct attgccctgc gctgatggcg tggtattgcc
     1081 cagtatttct gaggatttca taaccaccga aaagtccccc ccattattaa tactgctgag
     1141 aatattttta gaggtctgat tattggtggt tagggttgca cttgtgagcg cagcggctga
     1201 gtatactttt ccgctcacca gcacatcacc actggcgctg ccgccgttga cgggggttag
     1261 cagcagggtt gctacgctgg gttttttata gataaattcc gtttttactg cgctggataa
     1321 gccttggctg tcggtaatac tcaccgcgac ggaattaatg ccgtcattta attggctggg
     1381 ggaaacataa taattgtact gattcccagt tatgtattga gtaatgctaa tggccgctgc
     1441 gccgttaatc caaaggtcga gggctttaag tgactgtgcg cccagatcaa aattgcctga
     1501 gaccgataca aaatcggtaa ccgttgagcc ttctacgggc gtggctagat tgagtttggg
     1561 gggcagtgca taaataaagg cacgggtttg cgagccgcta aatgcagcgg cgcccgccac
     1621 gctggcaaca gcggtaactg ttgcggtgtg gccgccggct gaaagcgtat ttattggaat
     1681 ggcaaaatca aaattagggc tttgcgcagt gttgataata ttggcttgat taatagcatc
     1741 aaccgccagc tgaatcgtgc tcagcgcaaa tatagaattg actgtgccgg taatatgcac
     1801 ggtgccatta accgtttgat tagcgcttgg tgcgctaatc gtaacggttg gtggcgccac
     1861 cgctattttt tgcaactgca cctgtgtgac ggcgcccagg ttagaggttg ccgtaatcag
     1921 cagtgtattg tcggcgggtt ttaatccggc agtggtaaat gccagtgtaa acacgccgct
     1981 ggtttggctc gcgtttaaca gctgggggct gccattatta atgctgattt ccagcttggc
     2041 tgtttgttgt gtgggcagat caagggtgcc ggataattgc gctttgctca gggtgtcgcc
     2101 atctttaagg gcaaggctga tagagggttt ggttggtgca aaatcaaacg tatattgccc
     2161 ttgtccggtt tgattagcat cgttaacagc tttaatggtg agcagattgc ctgtgggtat
     2221 taacaggttg ctcggcaggt caaaactaaa attgccgctg gcatcggttt taaacgaggc
     2281 aactggattg gcagtgggca gcgtaaggct ccaattagta gtggctattt tggttttacc
     2341 ataggtgctt acatagccct gcaccgtaat cgcatccgtt tgcggctgtg aaaaatccgg
     2401 tgttacttgg gtgattaccg gtgttgtatt gggccgattc gtcgcaatta acgatgctgt
     2461 tcccacttgg ccattactgt ctttagcttg cagaactaat gtgttgctgc cagaagccaa
     2521 cccattttgt ttgacgacaa attcagcgct gcctgcagtg ggcgacgcga taaaactcgg
     2581 ctcaatttcg ctgctttcac gctctaaaat ataaccggca ataaattggg tttgagtagt
     2641 ggcaactgcg gaccagcgcc aattctgatc cttttgcata acgcccaccg ccaaactttg
     2701 aagcgcgggc tgtccgaacc accaggcgga ataattcacc atggtattgt ccgcccacaa
     2761 ccagccgcca ttggcatttt gtctggcgcc cataataaag ctgctgttag ctgcagcatt
     2821 cgggaagcgg ctggttaatt cgccagctaa ataatcctgc tcttcttggt tgttaataat
     2881 ggctaaatgc cagcctgttg gaatatcagt ttgtatggtt tgccaacttt tattcgctgc
     2941 ggcaataaat tgatattgag aaataatctg cgcttgatta tttaccagca caacaaggct
     3001 gcttaatttg ctgttggggc tgagggttag atttgcgttc actttaaaat cgccccgcac
     3061 aattaccggc gattgcggcg cagtaatcgt gacaattggc gcaccggtcg agctgctgga
     3121 ggaagagctt gagctgctgg atgaacttga gctgctggac gagctggaac tagaactgct
     3181 cgatgaactt gagctagtgg atgaactaga gctggaactg ctcgaagagc ttgagctact
     3241 ggacgagcta gagctagaac tgcttgatga acttgagctg ctggatgagc tagagctaga
     3301 actgctcgat gaacttgagc tagtggatga gctggagctg gaactgcttg aagagcttga
     3361 gctactggac gagctggagc tagaactgct tgatgagctt gagctagtgg atgagctgga
     3421 gctactggac gagctagaac tggaactgct cgaagaactt gagctagtgg atgagctgga
     3481 gctactggac gagctagaac tggaactgct cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnaagaactt gagctactgg atgagcttga actggaactg cttgaagagc
     3661 tggagctacc ggatgagctg gaactggagc tactggatga gcttgaactg ctcgaagagc
     3721 tggagctact ggatgagcta gagctggagc tgcttgaaga actcgagctg ctggacgagc
     3781 tagaactgga gctgcttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     3901 cttgaagaac tcgagctgct ggacgagcta gaactggagc tgcttgaaga actggagcta
     3961 ctggacgagc tagaactgga gctactcgaa gaacttgagc tactggatga gcttgaactg
     4021 gaactgctcg acgaactaga actggagctg ctggacgagc tagaactgga gctgcttgaa
     4081 gaactggagc tactggatga gctagcgctg gaactgctcg aagagcttga gctgctggat
     4141 gagctagcgc tggaactact cgaagagctt gagctgctgg atgagctaga actggaacta
     4201 ctcgaagagc ttgagctact ggatgagcta gaactggaac tactcgaaga gcttgagcta
     4261 ctggatgagc tagaactgct ggatgaggag ccgataactt ttgggtcaaa aattatcctc
     4321 acagtaaaac ctgtgccagc cggatattgg cccttaaggt tcaaataaac ttcttgcgct
     4381 gtatgggtta gcgcaatctc catccctgtg gtgttatcaa ttaggcttgc gcttctaatc
     4441 ggattatcaa accccgagag tacgaagccg tcaaaaacat cggtatccgc aagataaacc
     4501 gatggctcag gcagaataaa tttaatctca ttggcttctg gatcaatcag tgcgtgttcg
     4561 gtttgattgg ggccggcatt ccagtgaatt tggtctgcag tgcctgcggt tacgcgcgtt
     4621 gatacggagg ttggattcca tgttgtgtag ggtgtggttt gggttgggta acttctggtt
     4681 atcaccaagg tatttcccaa catgttgcca accaagtcag agccagagga tgagcttgtg
     4741 ctgctggacg agctggaact tgagctactg gacgagcttg aggaagaact agaagaacta
     4801 gaagagctag agcttgcggc cgatgaggcg cttgagcttg acgagctgga ggcggtgccc
     4861 gcgtctgcct cagtgacatc gaaactaaaa atatcctggc tgtcgccatt gccgatggag
     4921 cctgtcagct gggtgctgtt ttcaccccaa taaaccctgg ccgtatagtc agagcccagg
     4981 cctgccacgt gggttaagcg cacggtgtaa cgccccaccg gtgccacctg cggccagaag
     5041 atattctgct gggggtcaag gcttgagagt tcctcaaaca cgccgcgggc acctaggcat
     5101 tcggcctcaa agcggttggg ggcgatccag tgggcgcagg gatcttgtac ggccaactcc
     5161 aagcggctgg ccgcatccga ccacaccagg ttcacccgca cctggccagc ctggctgctc
     5221 agttggtttt gcggtttatc ggcattgggg gagggggtgg gtttggggct ggtgggcggt
     5281 gaggtgccgg tttcgttatc ttgtatgacg gcaacgccga tggcattaaa gggggcttgg
     5341 gccgtgccac caatgacccg cagggaaaaa tactcgcctt tttcacgggt tttatcgtcg
     5401 ataaccgtta ctgagacaat tttttcctgc tcgcctgggt tgaacactaa cttggccgcc
     5461 accggcacat agtcgctacc ggctttggcg gtgccatccg ccgtggtgta gttcaattct
     5521 gttacttggc tggcgggttt attcaggcgc acggtgaaat ccacccggcc ggtattttcc
     5581 gcctggccgc tgctcaccac cttggcggcg ttattcaggg gatagataaa cagatcgagg
     5641 gtatcggagg ccacctgctg gtcggcggtg gaggcgctga ggcgaaaact gagtttggtg
     5701 tattggctta catccggcac cagcacttgg gtttgcaact ggtcggcatt gacgaggctg
     5761 gcctgggggc cgtctatttg cacccagcgg gtgcgcgcaa agcttacatc gctgggcgct
     5821 gagccttgca agtccaccaa taaattggca tcgctgaaaa tatccggccc cgcatccacc
     5881 gaaaggcttg aattggcgct gggtttggtt gggttcgcca cctgcagatc gttacccgcc
     5941 ttgacgcgct cggcctcctc tcgcgcctgg gcctgtgccg gtgtgagctg ggccttagcg
     6001 ggatcgacgc ggctactttt gccctcaccg cagccgctga taacgagtgc gaacaggggg
     6061 gcgagcagcc agcggctggg cagggacttg aacatggcag atcctcaaca aatgctttta
     6121 aggggcaaac ctaaaaattt cgttaaatcg ggtgtagctg ttggctgggc agcgctggtg
     6181 gaactattcc ctggtgcaaa atggccgtgc ggcagcgggg ttttatacaa actgctgagg
     6241 tgaaatccgt ttgtatttac tttttgatca ccccacagcc tgaccctcag cccgagcaca
     6301 acacaaaaag gtgcgccctg ccgcacggat gggcaaaaat agtaggtaat taatatggct
     6361 aaactattaa gcgggcaccc atagccgcca acttaaagcg gctgtgttca ccaatctgcg
     6421 cctaagtcgc gcaaacttcg cggcctggag cggcaggttt tagcggcggc aggctaagcc
     6481 tgccaatcct ctgcaatttc catggtaaaa agttgcgcta gtgtaggaag cttgccttga
     6541 aattgccaga aattggtcta gcgcacttat tcgattatct aatactgcaa tgggcgccgc
     6601 agccagtggg ttttttacct gatgaccggg ctaaaaaaag ctgacgaact gtttaacagc
     6661 gcgtgggtaa ttcacaaaaa gaatgtgacg gctgccgcga tgatggttta tgcccactgc
     6721 tgcgcaaagg aactgctgtt tgcctgcggg cacctaaaca aacaaataaa ctgccagggc
     6781 aataaatacc cctaaaaagc cgatgctggc cagcaggggc cagctcgatt gggtgggttc
     6841 caccgggtcg atgcgattgc ccggtgaggt gggttttaaa tcgccccggc gggccggtgc
     6901 gcgcagttgg gttttggcct cagcggggct ggggccgatt attttgaagg tgtacacgtc
     6961 caggcgcagt ttatcgccgg gtacggcttt actggtgtgg atgcgggttt cgttgacaaa
     7021 ggtgccgttg gcggaattta aatcttccaa ctccaggtgg tcttcacgca gggtaatggc
     7081 taggtgttgg cgggaaaggt gagtgccggg gaacaccaaa tcacaatcct tgccgcgccc
     7141 aagtgtcagg cgggtgctgc cgtccgtggc gccgggcagc gggaattcct ggccggataa
     7201 aaaattcgaa caggccacta acgaccaact gcgcaggccc gatttcactc cgtccaggct
     7261 cgccataggg tccaccaggg tgagttttat ttcccctaac cgcagttgat ctccggcggc
     7321 aattttggct tgggagaccg gctcgccatt tacataggtg cctaagtggc tgccattgtc
     7381 tttgattacg aacccgtcgc tggtggcgag aatacgcgcg tggcgggggc tgattgaagg
     7441 gtcgttgaga atcagctgat tgtcgtcacc actgccgatg gctacttgtt tgtcggttag
     7501 ccagtaagct tcaaaccgcg ggtctgcaaa tacaattttt aacatagtcc cgaaaaccta
     7561 aagttgtacc cttagtttag gtagcaaaac cgcagagtat aggctgtagc acagcgtaaa
     7621 tggcttggtg ccgccgctgc agatgagtaa agcccttggc gtcctattca gtggggcggc
     7681 cattatgcca aagcttgctg ctagcgcaca gttatcacgg cggtggtatg tgtgagggtg
     7741 cgtgcaaaaa agtgacagcg cctgtggttg atctgtgcag gctgtaacga tctaacacat
     7801 ttttctgtgg tgccgagata aattcgctag aatggcgacc ctttttggcc cgttctacgc
     7861 tcggtgccat caacatctgc ccattaaaac accgccccct ttacgcgccg aggtaattat
     7921 ggaatcgcaa caattgccca gagtggttat tcactactgc accctgtgtc aatggcaatt
     7981 gcgcgcggcc tggatggcgc aggaactgtt gagcagtttg tccggcgagt tatttgaagt
     8041 ggccttgcga ccggggcagg gcggtgtgtt tgagatttgg gtaaatgaac atttggtgtg
     8101 ggagcgcaaa cgggacggcg gctttcccgg tgcaaaagag cttaaacagc gggtgcgcga
     8161 tgtggcctgc ccggaaaaaa tattaggcgc tcacctagag cgcggcgcag aacccacgcc
     8221 gtcgggcacg caggaataag gaacacataa caattatggc gcacagtcat ttcacccacg
     8281 cctgggtttc ttgggtaata aaaaatcccc gctggaccct gctgctcctg gtggtgttaa
     8341 ccctcgccgc agccgcaggt ttgccgcgat ttaagctcga tgcctcggcg gactccctca
     8401 ccttagaggc cgataaggat ttagattatt tccgccaagt gagcaaacaa tttaattccg
     8461 gcgatttttt agttgtcacc tatacgccta agcaggattt attctccgac gccgcgttgg
     8521 atcacttggg caaattacac gctgagcttg cggccatcga aggggttgca acggtaaatt
     8581 cgattttaaa tgtcccgctg ctatacagcc ccaaacaaaa cctgcgggaa atcgccaaac
     8641 agccgcgcac cctgttacct caagatccca cgcaaacgcc aacggataga gcgctggcca
     8701 aacacgaatt tttaaccagc ccaatttacc gccaattaat cctcagcccc gatgggcaaa
     8761 ccaccgctct gcagctgaat ttaagcgtgg ataaaaccct gattgatttg gtcagccagc
     8821 gcgatgcact gcttaccaaa cgccatcaag agggattaaa tcaaagcgaa gagcaattgt
     8881 tatctaaggt ggccgctgag tatttacagc tgcgcaccca ggcggatgcg cgctcccatg
     8941 cgcgggtagc caaagtgcgc gaagtggtgg gccgctataa aaacgaggcg caaatatttg
     9001 tcggcggcct caccatgata accgccgata tggtggatta tattcgcagc gatttaatga
     9061 tttttggcac cgcatcgttt ttatttatgg tgctgatatt ggcggtgtta tttcgcaacc
     9121 tgcgctatgt attaatcccc atcgccagct gtgtcatgtc cattgtgatg atgctggggt
     9181 ttttagcttg gatcgactgg cgtttaacgg tgatctccag caattttgtc gccctattaa
     9241 tgattatttc cctcaccgtg accattcatt tggtgatgcg ttatcgggaa tatgtggcga
     9301 gccatcccag ttggagccgg gagcagctga ttatggccac tctttctttt atggccatgc
     9361 ccagctggaa taatacgctc accaccgtgg tggcatttgc ctcgtttgtt actagcggta
     9421 ttcggccgtt aattgatttt ggctggatga tgtccatcgg tttgatggtg gcgttagtct
     9481 tgtcgtttgt aactctgccc gcgtttttga tgttattacc aaaggataat gcagctcccg
     9541 ccgtgcatcc gctcaccaaa aatcgccgct ccatcagcgc atggtgtggt gtattggtgg
     9601 aaaaatgcgg cccgggtatt ttttggggcg gcttgttatt aggggcgctg agtttttggg
     9661 gaattgctaa attggaagtg gaaaaccgtt ttatcgatta tttccacgcg gatacggaaa
     9721 tccaccaggg cctgtcttta atcgataata aactcggcgg caccaccagc ttggatatta
     9781 ttatcacccc aaaaccggct agcagtggcc caggcgggga tgccgatcct tttgccgacc
     9841 cggaaaaaga agccgacccg tttaataatg atgaagacgc cttcgccgat ggcgacccgc
     9901 tgtcggaaaa cgacgcgaaa accagtgtat ggatgacggt gggcggcatg gagtatgtgc
     9961 aaaaaattca cgattatttg gagaccttgc cggaggttgg taaggtgcag tccctcgcca
    10021 cgctgtataa agtgggcaag gatataaacg gcagcttaaa taattttgag ttggcattaa
    10081 tggaaaaatc cctgccggag gcggtcagcc gggttttatt taacccttat ttggataaat
    10141 ctacggatca aacccgcatc acgctgcgcg tcaaagattc gtaccctggc cttaaacgcg
    10201 ccgaattaat tgaacgcatc cgcaaccata ttagcgctct tgacggcatg gatattcagg
    10261 aggtgcgcta tagcggcttg ctggtgttgt acaacaatat gttgcagagc ctgtacacct
    10321 cacaaatcgc caccctgggt tggtcgtttg tggtgatatt tattatgttt ttggtgctgc
    10381 tgcgctcttt tttactctcg gtggtggcca ctgtacccac ggctatttcc gctgcggtta
    10441 tgctgggcgg tatggggttt gccggtattc cactggatat gatgaccatt accatcgcct
    10501 ccattaccgt gggtataggc gtggataata ccatccacta tttgcatcgc tttgaagagg
    10561 agttgcaact ggatggcgat tatttagcga ccatgcatcg ctgccacgcc tccatcggcc
    10621 atgctattta ccacaccaat aatattattg tgtttgggtt ttccattatg gtgctctcat
    10681 catttattcc caccatttat tttggcgccc tcacgggtgt ggccatgatg gtggctttgg
    10741 cgggcactat gtttttcttg ccgcaattta ttttgatgat taagccattt aaggtgttta
    10801 agacggcagc ttaaattgat aatgcaatcg gctattaaaa aatagtcaca aaaaacccgg
    10861 cgcaggccgg gttttttgtg ggcgctgttt gggtggccga ttagatgctt tataactgca
    10921 ataaacgaaa atacacatcc ttaggttggt gggtgcgatc aaataataag gggtgattgg
    10981 tgcggccttt aatggggaag tcgttcaacc aggtcatatc atcggaaaca ccccagaagg
    11041 tcacccggtc gattttatcc ttgtgtttaa tgaataattt aaacaaagac tcataacggg
    11101 cactcaactg atcgttgcgc tctttagtga tacctttgat ataggggtcc cgctcaggcg
    11161 tatagccaaa gcgggttgct atatcggcgg tggaggattc ccac
//
LOCUS       DAOQGS010000056        11099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2729_length_11099_cov_4.632923, whole genome shotgun sequence.
ACCESSION   DAOQGS010000056 DAOQGS010000000
VERSION     DAOQGS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_11099_cov_4.632923"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKZ52_03470"
     CDS             <1..483
                     /locus_tag="PKZ52_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HNG58859.1"
                     /translation="THDQEEAMEVADRIVVLNKGNIEQIGSPDDIYNNPASPFVYDFI
                     GQVNLFHSRVDNGWAHIGDYKLAAPEFNGVQNQNAFAYIRPHDIELSHEDSEGAIAAT
                     VAYVSTAGAVLRIELKHSLSDEIIHVELPRSQERALDLRPGASVFAKPTSSKVFLQPA
                     "
     gene            1285..2031
                     /locus_tag="PKZ52_03475"
     CDS             1285..2031
                     /locus_tag="PKZ52_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58860.1"
                     /translation="MSLHDLTQHFNDLLKQQQELLPHLPNTYFYWREGEFFARFGSQN
                     FGNQFIPILSAADKKIIGYESRLWVRTALGSAIPVDDVFDALTNLDEVVFLDRITRTL
                     QALNFSVLPEHNGALLSLGIQPRHILGVATGHGRTFDTILGDCGLATAKVILHTSIPA
                     PEHFAHFRRALFSYSAFGYRVGMHIKSAQDWERYQEWNLTPDYLFLPADVPLDFKKFA
                     GPQIIRQIDGGYELVDEALAEASAPSSAQE"
     gene            complement(2319..2735)
                     /gene="arfB"
                     /locus_tag="PKZ52_03480"
     CDS             complement(2319..2735)
                     /gene="arfB"
                     /locus_tag="PKZ52_03480"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNG58861.1"
                     /translation="MLWITPKLYLPMSCIEWTAVRAQGSGGQNVNKVSSAIHLRLDIA
                     ACPLPAEMKERLLAISDKRLTREGVLIIKAQQHRTQELNRADALERLTQWLREGIHQD
                     PQRIPTRPKASARYKRLASKAHRSECKSLRRRPVGE"
     gene            3402..5516
                     /gene="malQ"
                     /locus_tag="PKZ52_03485"
     CDS             3402..5516
                     /gene="malQ"
                     /locus_tag="PKZ52_03485"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNG58862.1"
                     /translation="MEHFDKLFYWHGIADSYYNYRGDLVTVPFENKLNIINAMGVDTS
                     DPEKTAQDAYRLDVAPWQQWLLDFSVCYQGPEQVFFLQVQPGELELEFEFDLQQNGLS
                     LASGTVVPAQFAEVGDYAFEGVRYSRRAIPLPALPLGYHTLSVSCGGKTQSACLAVAP
                     PKAFVSPALQKGERVVGIIVQLYTLTSSRNWGIGDFTDLALLIRKAAALGVQMIGLNP
                     LHALLEPVDHNCSPYSPSDRRFNNPLYIDPEQESDFRLDIKNFAQELKLLRQEKNVPY
                     GPVHKLKYACFRQMFEVFCQVELGSNSQRDQAFQLFVETQGTALEDFCIYQVAQELSL
                     PVPWEDEAAKSAFIAEHRSKAQFHAYLQWLADKQLAACQQLAESLGMGIGLMRDLAVG
                     ANGSGSEVSSHLDLFCREAAVGAPPDPLAEQGQNWGLPPMDPASLRLSRFSHFITLLR
                     TNMAHCGALRIDHAMSLLRLWWCPPGTTADHGAYVYYPFHELMALLKLESHLNSCMVI
                     GEDMGVVPPQLRQALYDGDIFTNKLFYFEKDPQGQFKHPDHYEAHSLAMVTNHDVPTL
                     AAWWNGQDLLLRQELKLYPPEVNLGDLQAGRRYEKSQLLNWLIFLGFALPGAFEAVLD
                     GPLTWELCALILKGANRCRSQLFVVQLEDLELLDTPVNVPGTCFEYPNWQRKLAKPLD
                     AVFADAQVGQLLRDLVAARAAS"
     gene            5548..7734
                     /gene="glgB"
                     /locus_tag="PKZ52_03490"
     CDS             5548..7734
                     /gene="glgB"
                     /locus_tag="PKZ52_03490"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNG58863.1"
                     /translation="MTTAVATTAVEALIHAYAEDVFSILGLHERQGRWHVTCFLPDAL
                     QVEVVDEAERPLGALNLIHKDGVFDGPVNLAARAPYKLRVHYAAGVYIKEDPYRFGSS
                     LTPLDLYLFGEGTHERLYRFMGAHVISQEGVWGTRFCVWAPNAQRVSVVGEFNFWDGR
                     HHMMRKHIPSGVWELFLPGVSAGALYKFEIRCRDGRVLPQKADPYGFAAQQPPETASQ
                     VVAPHFAWSDELWRSSQAQRKSLQEPLSIYEVHLGSWKRVPEEGNRYLTYKELAQQLI
                     PYVKSLGFTHIQLMPISEFPFDGSWGYQPIGLFAPTSRFGSPDDFRFFVNACHQADLG
                     VLIDWVPGHFPSDGHGLAQFDGTPLYEHADHRQGYHPDWNTYIYNYGRCEVANFLYSN
                     ALYWFDEFHVDGLRVDAVASMLYLDYSRNAGEWIPNAFGGRENLEAIDLLRRVNSKVY
                     EHYPHALMIAEESTAWPGVSQPVYNGGLGFGYKWNMGWMNDTLRYMHKEPVHRQYHHH
                     DMTFSLLYAFSENFILPLSHDEVVHGKGSLLDKMPGDGWQKFANLRAYFGYMWTHPGK
                     KLLFMGGEFAQGREWNHDASLDWHLKELECHAGISRLIGDLNHLYRQYPALHYSDARA
                     DGFEWMDADASHLSIFAYLRKATDLAPAVLVICNFTPVLREDFRLGVPEAGFYKEVLN
                     TDAAIYGGCDQGNGGGLHTEAVPSHGRPHSLKLKVPPLATLVLSLV"
     gene            7849..9957
                     /gene="glgX"
                     /locus_tag="PKZ52_03495"
     CDS             7849..9957
                     /gene="glgX"
                     /locus_tag="PKZ52_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNG58864.1"
                     /translation="MKTDKKHTIEQGRPYPLGSSVVPGGVNFALFSQHAEEVELCLFS
                     PDGQTEVARLLLPKCSNQIWHGFVPGLSAGAVYGYRVYGPYDPHRGLRFNPNKLLLDP
                     YAKALVGDFIWHDSHYGFTTSEPAQDLTYDDRDNAHWMIKGQVVAKVPPPTPLPAPIA
                     WEDTVILEAHVKGLTYKHPAIPLEERGTYKALAHPVMIEHYRKLGITSLELLPVHSFI
                     HDHFLIKRNLTNYWGYNTLNFFTPQRAYAASADVAEEFRQMVSALHEAGIEVILDVVF
                     NHTAEGSQLGPTLSFRGIDNSNYYTLLQQDARFYINDTGCGNTFNVRHPKVLQLVLDS
                     LRYWACDLGVDGFRFDLATVLGREAGGFEQGCGFLDALAQDPALAGKKMIAEPWDIGP
                     GGYQLGNFPPGWSEWNDRYRDVLRQFWRGDRGVLPELARRLHGSAELFEYAGRSPQAS
                     INFITSHDGFTMADLVAYNQRHNLANKEFNNDGHHANFSYNHGEEGPSNNPHINALRQ
                     RQQRNLLASLIFSQGVPMLLAGDELGHSQGGNNNAYCQDNATTWLNWAEADQQLVAFV
                     ARALSIRRAHPVLRTPFYIHKPDELGAASGFDIHWLDRQARPMRDADWQEQNLHSLQW
                     MLETEDAEACKQAVLLLLNADERTQTFYLPLGWRWECLLDSRHPAGEPVMAYLPKGSA
                     FMVHDKSLLLFFGQSLECSL"
     gene            9954..10556
                     /locus_tag="PKZ52_03500"
     CDS             9954..10556
                     /locus_tag="PKZ52_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58865.1"
                     /translation="MTPSRCGRDVINRIFTLGLFAWIATGDAYGLSSVRTQPVVLSKS
                     EFSLLEAVACQQHHQVAAVDIKAESNVDNPKSKTAVVTCASKQDFMGQAANNIVYCGA
                     KGARWECQKGPLRVQVPLQGRQVKLYLMRVAPQEAFEGLAKVITLQFQGYFIDKIVGD
                     SCDVSKNKFPAEIEFHCDGGKFVLSKYCLDTNCPRILSVN"
     gene            10730..>11099
                     /locus_tag="PKZ52_03505"
     CDS             10730..>11099
                     /locus_tag="PKZ52_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HNG58866.1"
                     /translation="MKSKKTAFTLGIDAISLATDVDRHYNFSLGRCDEAPVSNYVHHA
                     LSLSVRDRLMERWRKSREQEQRTKPKRVFYLSLEFLLGRTLSNAIKNLAMEASTQEAL
                     HELGMTLEDVRENELDAGLGN"
BASE COUNT         2331 a         3026 c         3108 g         2634 t
ORIGIN      
        1 acccacgatc aagaagaagc catggaagtg gccgatcgca ttgtggtgtt aaataaaggc
       61 aatattgaac agatcggctc gccagacgat atttacaata atccggcaag cccctttgtg
      121 tacgatttta tcggccaggt gaatttattc cacagccggg tggataatgg ctgggcccat
      181 attggcgatt ataaattggc ggcgccggaa tttaatggtg tgcagaatca aaatgcattt
      241 gcttatattc gcccccacga tattgaatta tcccacgagg attctgaggg cgccatcgcc
      301 gccaccgtcg cctatgtgag cactgctggt gctgtgttgc gcatcgagtt aaagcacagt
      361 ttgagtgatg aaataattca tgtagaactg ccgcgctccc aagagcgagc attggattta
      421 cgccccggcg ccagtgtatt tgccaaaccc accagctcca aggtgttttt acaacctgcg
      481 taatatgtgc cgctgtgctc tacactgagg cagcggcggc ttttatttgt atgcaaagca
      541 taagttaagt tgttttgtgc cctacacatt tttagttgtt gcacgcgctt tatggccatc
      601 tccatgccgg tggcaaatgg agggcaattt agccttgcgg cataattaat tcgcggtgca
      661 gcaggagtga atatcgaggg ctcgcaacac ttagggaacc tctgaaaaat taccttactc
      721 gccctggcgt cgttgaattt gccaaaaaaa tgctcattta ctgcgtgtaa actccgcttt
      781 ttttggcaaa ttcgcctagc cagcgcttcg ccgcctacat ttttcagagg ttccttagca
      841 tccgctggat ggtttcgagt tggcgcgtga atcactaaaa gttgaaagga gccgaattgt
      901 taccgcgttt tttaatataa gctaagaagc aaattatatt tatcataatc taatcgttcc
      961 gctattatta aagcagtgta tacaccgcga caattataaa acgccatgga tgccttttca
     1021 tctgtacttt ttataattag tcatttcgcg tcctggttat caggatgctg ttgcgatggt
     1081 tgttgcgctg tttttttgtc tatttagtag atgctactac tcaatgctca actactacgc
     1141 aatgctccac tactaagcag tgcccaagaa cgggaacttc tgatcacttg atgtgttcaa
     1201 tgccactccg tgcgtttttg aaaacatcaa ttaatctggg catccttttt ttggctgcgc
     1261 accatccgcc gacacaggag ttctatgtcg ctacacgatt taacccagca ttttaacgat
     1321 ttgcttaaac agcagcagga gttgttgccc catttgccca atacctattt ttattggcgt
     1381 gaaggtgagt tttttgctcg ttttggcagc cagaacttcg gcaatcagtt tattcctata
     1441 ctctcggcag cggataaaaa aattatcggc tacgaaagcc gcctgtgggt gcgcacggcg
     1501 ctgggttcgg ctatacccgt tgatgacgta tttgatgcac tcaccaattt agatgaggtg
     1561 gtgtttttag atcgcattac ccgcacctta caggcgctta atttttccgt tctgcctgaa
     1621 cataacggcg cattattatc attgggaatt caaccccggc atattttggg cgtggcaacg
     1681 ggccatggcc gtacctttga tactatttta ggggattgtg gcttggctac cgccaaagtc
     1741 atattacaca ccagcattcc cgccccggag cattttgcgc attttcgccg cgccttattt
     1801 agctatagcg cgtttggtta tcgtgtgggg atgcatatta aatcggcgca ggattgggag
     1861 cgttaccaag agtggaattt aaccccggac tatttatttt tgcctgcaga tgtaccgctg
     1921 gattttaaaa aattcgccgg gccgcaaatt attcgccaaa tagacggtgg ttatgagctg
     1981 gttgatgagg cgcttgcaga ggcttcggcg ccgtcatctg cgcaagagta gcctgactat
     2041 tattcgcggt gaatttgtgc ttgttaacaa atttctaatt aaatctgtat ttgggaatat
     2101 tttgacatca cacgaaagcc tggtgccgat ttttgcctgc tgttgtattt acccaaaaac
     2161 ccgtcaaccc ttctgttaaa aagctaatgt cgtttaattt aggcggggta gtaggggcaa
     2221 cccttgtgat tgcccagtgt gcgacttggc taaagctcaa tatttacgtc gaagcgggca
     2281 cccacaaggg gcgccccttc cgcccagctg aatactcctc actcccccac cggcctgcgc
     2341 cgcaggcttt tgcactccga tctatgagct ttgctcgcta agcgtttgta gcgggcggag
     2401 gctttggggc gggtggggat acgttgcggg tcttggtgga tgccctcgcg cagccattgg
     2461 gtgaggcgtt caagggcgtc ggcgcggttg agttcttggg tgcggtgctg ttgggctttg
     2521 atgataagta cgccctcgcg ggtgaggcgt ttgtcgctga tggccaggag ccgttccttc
     2581 atttctgcgg gcagggggca ggcggctatg tccaggcgca ggtggatggc gctggagacc
     2641 ttattaacgt tctggccgcc gctgccctgg gcgcgcacgg cagtccattc gatgcagctc
     2701 atgggcaaat agagtttggg ggtaatccac aacatagggg gcagcttcgc aggcttttgc
     2761 tcagtgtggc agccggtggc ggtttaggcg aggggcaatg tcattaagct cagcgcctcc
     2821 taagatagtc gatagccgta ggggcgcccc ttgagggtgc ccgatcccgc gaagattccg
     2881 atggatcgct cagatccaac agggcaaccg caaggcttgc ccctacagcc tgttttaacc
     2941 taaggactta aacgaattgc cttgctaaac agccaattca aaccggtttt ggtttgccgc
     3001 aaaccggctg gggcggagtc tgctacattt atgttctttt gccgcgctgc gatgctacat
     3061 tttaatgcgt taatgaccag gtgatttcgg gcttgcaggg ccgtgcccta aagtggtaat
     3121 ttcggtgcgc agtgttggtt tattgatttg cttaatttgt gtacagatgt gggtgtaaat
     3181 agcagcttgt ccctgtgggg gtttgagcca gccgcagggc gggatctatt cggcggctgg
     3241 ggccgcgttt tggtgctgct tttgcactga gatttgtttg ccgctggcag cggcatgtag
     3301 cgtgcggcta ttttttgcgc cttttttagg ggctttttta tttagagtct ggggccatag
     3361 cgtgtgtatg gcgttaatta acttggtttg caggctgctt tatggagcat tttgataagt
     3421 tgttttactg gcacggtatt gccgattcct attacaacta ccggggggat ctagtcacgg
     3481 ttccctttga aaacaagctc aatattatca acgccatggg tgtggatacg tccgacccgg
     3541 aaaaaaccgc ccaggatgcc taccgcttgg atgtggcccc ctggcagcag tggttgctgg
     3601 atttttccgt ctgttaccaa ggccccgagc aggtgttttt cctgcaggtg cagccgggtg
     3661 agttggagct ggagttcgaa tttgacctgc agcagaacgg cctgagcctc gcctcgggca
     3721 ctgtggtgcc cgcccagttt gctgaggtgg gggattatgc gtttgaaggc gtgcgctata
     3781 gccgccgggc catccccctg ccagccttgc ccttgggcta tcacacctta agtgtcagct
     3841 gcggcggtaa aactcagtcc gcctgtttgg cggtggcgcc gcctaaggcc tttgtaagcc
     3901 ccgccctgca gaagggtgag cgggtggtgg gcattattgt gcagctctat acgctcacct
     3961 ctagtcgcaa ctggggcata ggggatttta ccgacctggc gctgttgatc cgtaaggccg
     4021 cagccctggg tgtgcagatg attggcctta accccttaca cgctttactg gagccggtgg
     4081 atcacaactg cagcccctac agcccatcgg atcggcgctt taataacccg ctctatatag
     4141 acccagagca ggagagcgat tttcgcctgg atattaaaaa cttcgcccag gagctaaagc
     4201 ttctgcggca ggagaaaaac gtcccctatg ggccggttca caagctcaaa tacgcctgtt
     4261 tccggcagat gtttgaggtg ttctgccagg tggaattagg cagcaactcc cagcgcgatc
     4321 aagccttcca gttatttgtg gaaacccagg gcactgcctt agaggatttt tgtatttacc
     4381 aggtggccca ggagttaagc ctgccggtgc cctgggagga cgaagcggcc aaatccgctt
     4441 ttattgccga gcatcgcagc aaggcgcagt ttcacgctta tttacagtgg ttggcggata
     4501 aacaattggc cgcctgccag cagttggctg aatccctcgg catgggcata ggcctgatgc
     4561 gggatttggc ggtgggcgcc aatggctcag gttctgaagt gagcagccac ctggatttat
     4621 tctgccggga ggccgccgta ggcgcgccgc ctgacccctt agccgagcag ggccagaact
     4681 ggggcctgcc gcccatggac ccggccagcc tgcgcctgtc ccgctttagc cactttatta
     4741 ccctgttgcg aaccaatatg gcccactgcg gcgccctgcg catcgaccac gccatgagcc
     4801 tgttgcgcct ctggtggtgt ccgccgggca ccaccgccga ccacggtgcc tatgtgtatt
     4861 acccctttca tgagctgatg gcgctgttaa aactggagag ccacctcaac agctgcatgg
     4921 tgatcggcga ggacatgggt gtggtgccgc cccagttgcg ccaggccctt tacgatgggg
     4981 atatattcac caacaagttg ttttattttg agaaggaccc ccagggccag tttaagcatc
     5041 cggaccatta cgaggcccac tccctggcca tggtaaccaa ccacgatgtg cccaccctcg
     5101 ccgcctggtg gaatgggcag gatttgctgt tgcgccagga gcttaagctt tatccgccgg
     5161 aggttaacct aggggatttg caggcgggcc gccgctatga aaaatcccag ctgctcaact
     5221 ggctgatctt tttaggtttt gccctgcccg gtgcgtttga ggcggtgctg gatggccccc
     5281 ttacctggga gctgtgcgcg ctgatcctca aaggcgccaa ccgctgccgc tcccaattgt
     5341 ttgtggtgca gctggaggat ttggaattat tggatacccc ggtgaatgtg ccgggcacct
     5401 gttttgagta ccctaactgg cagcgcaagc tcgccaaacc cttagacgca gtttttgctg
     5461 acgcccaggt gggccagctg ctgcgtgacc tagtggccgc acgggccgca tcctaactac
     5521 acacctgcta tggggaacct atttcacatg acgacagccg tcgccactac cgccgttgag
     5581 gcactaattc acgcctatgc cgaggatgta ttttcgattt tagggttaca cgaacggcag
     5641 ggccgctggc acgtgacctg ttttttaccg gatgccctgc aagtggaggt ggtagacgaa
     5701 gccgagcgcc ccctgggtgc cttaaacctc atccataaag acggtgtatt tgacggcccg
     5761 gtgaatttag ccgcccgggc gccctacaaa ctgcgggtgc attacgccgc aggggtatac
     5821 ataaaagaag acccctaccg cttcggctcc agccttacac cgctggattt atacctgttc
     5881 ggagagggca cccacgagcg cctctaccgc tttatgggcg cccatgtgat cagccaagag
     5941 ggggtttggg gcactcgttt ttgcgtatgg gcacccaacg cccagcgggt ttccgtggta
     6001 ggggagttca acttttggga tggccgccac catatgatgc gcaaacacat tcccagcgga
     6061 gtgtgggagc tgttcctgcc cggcgtgagt gccggtgcgc tgtataaatt tgaaattcgc
     6121 tgccgggatg gccgggtgct gccgcaaaag gcggaccctt acggctttgc cgcccaacag
     6181 ccgccggaaa ccgcctccca ggtggtggct ccccacttcg cctggagcga tgagctctgg
     6241 cgcagcagcc aagcccagcg caagagcctc caggagccgc tctccattta cgaggtgcac
     6301 ctaggttctt ggaagcgggt gccggaggag ggcaatcgct acctcactta taaggagctg
     6361 gcccagcagt taatccctta tgtgaagtcc ctaggcttta cccatattca gctgatgccc
     6421 atcagcgaat ttccctttga tggctcctgg ggttatcagc ccatcggcct cttcgccccc
     6481 accagccgct tcggttcgcc ggatgatttt cgcttttttg tgaacgcctg ccaccaggcg
     6541 gatttagggg tgttgatcga ctgggtgccc ggccacttcc cctccgacgg ccacggcctg
     6601 gcccagtttg acggcacgcc cctgtatgaa cacgccgatc accgccaggg ctatcacccg
     6661 gattggaaca cctatatcta caactacggc cgctgcgagg tggccaactt cctctacagc
     6721 aacgccctgt attggtttga tgagttccat gtggacggcc tgcgggtgga tgcggtggcc
     6781 tccatgctgt atttggatta ctcccgcaat gccggtgagt ggattcctaa cgcctttggc
     6841 ggccgcgaga acctagaggc catcgatctg ttgcggcggg tgaatagcaa ggtgtatgag
     6901 cactaccccc acgccttgat gatcgccgag gaatccaccg cctggcccgg cgtgtcccag
     6961 ccggtataca acggcggctt aggctttggc tacaagtgga atatgggctg gatgaacgac
     7021 accctgcgct acatgcacaa ggagccggtg caccgccagt accaccacca cgatatgacc
     7081 ttcagcctgc tctacgcctt cagcgagaat tttattctgc ccttaagcca cgatgaggtg
     7141 gtgcacggca agggttcgct gctggacaaa atgcccggcg acggctggca gaagttcgcc
     7201 aacctgcggg cctacttcgg ttatatgtgg acccatccgg gcaaaaagct gctgtttatg
     7261 ggcggtgaat ttgcccaggg gcgggagtgg aaccacgacg caagcctgga ttggcacctc
     7321 aaagaactag agtgccacgc gggtatctcc cgcctgatcg gcgatttgaa ccatctctac
     7381 cggcagtacc cggccctgca ttacagcgat gcccgcgccg acggttttga atggatggac
     7441 gcggacgcct cccatctgtc gatttttgcc tatttgcgca aagccactga cctggccccg
     7501 gctgtgcttg tgatctgcaa ctttacgccg gtactgcggg aagactttcg tttaggcgtg
     7561 cctgaggcgg gattttacaa agaggtgctc aacacggatg cggccattta cggcggctgc
     7621 gatcagggca acggcggtgg gctacacacc gaagctgtgc ccagccacgg caggccccat
     7681 tccctgaagt tgaaagtgcc gcccttggcg accctggtgt tgagcctggt ttagccgagg
     7741 ggttagttaa ttttttgcaa gagtgagcag tgaagacatg cgccagtacg actgcgcaaa
     7801 agggcaggga gcggccaagg tgatagtgct cgggtgaggt tgcagtaaat gaaaaccgat
     7861 aaaaaacata ccattgaaca gggcaggccc tatcccctcg gcagcagcgt ggtgcccggc
     7921 ggggttaatt tcgcgctctt ctcccaacac gccgaggagg tggagctgtg ccttttctcg
     7981 ccggatggcc agacggaagt ggcccgcctg ctgctgccta agtgcagcaa ccaaatttgg
     8041 cacggctttg tgccgggcct cagcgcgggt gcggtgtacg ggtaccgcgt ttacggcccc
     8101 tacgaccccc accggggcct gcgctttaac cccaacaaac tgctgctgga cccctatgcc
     8161 aaggccctgg taggggattt tatctggcac gactcccact acggcttcac caccagcgag
     8221 cccgcccaag atctcaccta tgacgatcgg gacaacgccc actggatgat caaaggccag
     8281 gtggtggcca aagtgccgcc accgaccccc ttgcccgccc ccattgcttg ggaggacacg
     8341 gtcattttag aggcccatgt caaaggcctc acctacaaac acccggccat cccccttgag
     8401 gagcgcggca cctataaggc cctggcgcac ccggtcatga tcgagcacta ccgcaaactc
     8461 ggcattacca gccttgagct gctgccggta cacagtttta ttcacgatca ctttttaatt
     8521 aagcgcaacc tcaccaacta ctggggctat aacaccctta atttcttcac cccccagcgg
     8581 gcctacgcgg caagcgcgga tgtggcggag gagtttcgcc agatggtgag cgccctgcac
     8641 gaggcgggca tagaggtgat tctggatgtg gtgtttaacc acaccgccga gggcagccag
     8701 ctggggccga cgttaagttt tcgcggcata gacaacagca actactacac cctgctgcag
     8761 caggatgcgc gcttttatat caacgacacc ggctgcggca acaccttcaa cgtacgccac
     8821 cccaaggtgc tgcaactggt gctggattcc ctgcgctact gggcctgtga cttaggggtg
     8881 gacggctttc gctttgacct ggctacggta ctgggccggg aggcaggcgg ctttgagcag
     8941 ggctgcggct ttttagatgc cttggcccaa gacccagcct tagccggtaa aaaaatgatc
     9001 gccgagccct gggacattgg ccccggtggc tatcagctgg gaaacttccc gcccggctgg
     9061 agcgagtgga acgaccgcta tcgggatgtg ctgcggcagt tctggcgcgg ggatcgcggc
     9121 gtgttgccgg agctggcccg ccgcctgcac ggctcggcgg aattattcga atacgcaggc
     9181 cgcagccccc aggccagtat caacttcatc accagccatg acggcttcac catggcggat
     9241 ctggtggcct ataaccagcg ccacaacctc gccaataaag aatttaataa cgacggccac
     9301 cacgccaact tcagctataa ccacggcgag gaggggcctt ccaacaaccc ccacattaac
     9361 gccctgcgcc agcgccaaca gcgcaaccta ttggccagcc tgatcttcag ccagggggtg
     9421 cccatgctgc tggcggggga tgagctcggc cacagccagg gtggcaacaa caacgcctac
     9481 tgccaggaca acgccactac ttggctcaac tgggccgagg cggatcagca actggtggcc
     9541 tttgttgccc gcgccctctc catacgccgc gcccacccgg tgctgcgcac gccgttttac
     9601 atccacaaac ccgatgaact cggcgccgcc agcggctttg atattcactg gctagaccgc
     9661 caggcgcggc ctatgcggga tgcggattgg caggaacaga acctgcatag cctgcagtgg
     9721 atgcttgaga cggaggatgc cgaggcctgc aagcaagcgg ttttgctgct gctcaacgcc
     9781 gatgagcgca cacaaacctt ttacctgccc ttaggctggc gttgggaatg tctcctcgat
     9841 agccgccacc cggcagggga gccggtgatg gcctacctgc ccaagggcag cgcttttatg
     9901 gtgcacgaca aatcgctgct actcttcttt ggtcagtccc ttgagtgcag cctatgaccc
     9961 ctagccgatg cgggcgggat gtcatcaaca ggattttcac cctgggacta tttgcctgga
    10021 tcgcaacggg agatgcctat gggctcagtt cagtgcgcac acaaccggtg gttttatcaa
    10081 aatctgaatt tagccttttg gaggcggtgg cctgtcagca acaccatcag gtggccgctg
    10141 tggacattaa ggcggaatcg aatgtggaca accccaaatc gaaaacagcc gtggtgacct
    10201 gtgcatctaa gcaggatttt atggggcaag cggctaataa cattgtgtac tgcggtgcta
    10261 agggtgcgcg ctgggaatgc caaaaaggcc ccctgcgggt gcaggttccc ctgcaggggc
    10321 ggcaggtaaa actgtattta atgcgtgttg caccccagga ggcgtttgag ggtttggcaa
    10381 aagtcatcac attgcagttt caaggctatt ttatagacaa aattgttggc gactcctgcg
    10441 atgtttcgaa gaataaattt cccgctgaaa ttgaatttca ctgcgacggt gggaagtttg
    10501 tgctttccaa atattgcttg gataccaatt gcccaaggat tctttcggtt aattaatgga
    10561 tcagcaaaaa aaaacagagc aatacatgca tcagcaacag gcgataaata agcgataaat
    10621 aagtgataaa taaaaagtaa cgatacacct atgtagtcgg gccctgccct gaaatgttgt
    10681 cgggctctgc cctgaagttg tctaactctt aaatagctag ggattcccta tgaagtcgaa
    10741 aaaaaccgca tttaccctcg gcattgacgc tataagcttg gctacggatg tagaccgcca
    10801 ctacaacttt tccctcggcc gctgcgacga agcgccggtc tctaattatg ttcaccatgc
    10861 actgagctta agcgtgcgcg atcgcttaat ggagcgctgg cgcaaatccc gcgagcagga
    10921 acagcgcacc aagcccaaac gcgtgtttta tttatcccta gaatttttat tgggccgcac
    10981 cctcagcaac gccattaaaa acctggccat ggaggccagc acccaagagg ccctgcatga
    11041 gctgggcatg accttagaag acgtgcgcga gaacgagctg gatgcgggcc tgggcaatg
//
LOCUS       DAOQGS010000057        11085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2734_length_11085_cov_4.352675, whole genome shotgun sequence.
ACCESSION   DAOQGS010000057 DAOQGS010000000
VERSION     DAOQGS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2734_length_11085_cov_4.352675"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKZ52_03510"
     CDS             <1..533
                     /locus_tag="PKZ52_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG58867.1"
                     /translation="ATAIVREREQGNMEFLITTPIHPVELMLGKIIPYVLAGLVQSSI
                     ILSLGHSLFGVPINGSLLALFIVVITFILASLTLGLIISTLVQTQLQAMQMTVFILLP
                     SILLSGFMFPYEAMPVVAQYVAELLPATHFMRCIRAVVLRSASLGDLAFDLSWLVLFC
                     FAGLLVASLRFKKRLD"
     gene            complement(612..1463)
                     /locus_tag="PKZ52_03515"
     CDS             complement(612..1463)
                     /locus_tag="PKZ52_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNG58868.1"
                     /translation="MSNLTTRTLTQASYRLGKCLPWVAALAAMSWMSSTWAGEFKWPK
                     KYQAAVSLSYDDTLDSQLDNAVPSLNKFNIKGTFYLIEAAPALQTRLEAWRKVAQQGH
                     ELGNHTLFHQCSKSQPGRDFVLEKNNLDKISLDQMVDQVKLANTFLFMLDGKTERTMT
                     TPCLDKKVANGENYVEAVRTLFVGIKNTGGSGVTEDMNTLDPAYVSVTLPSNITGKEL
                     IAIVEQAVAKGTMANFTFHGINGDYLTVSNQAHEELLSYLAAHKDTIWTDTFINIMKY
                     VRKERKI"
     gene            complement(1583..3220)
                     /locus_tag="PKZ52_03520"
     CDS             complement(1583..3220)
                     /locus_tag="PKZ52_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNG58869.1"
                     /translation="MKLVLALLFSLCPLWAYAEGGAPDHTLAIIMFVVFVAITLGITY
                     WAKNRTKSAADFYTAGGGITGFQNGLAIAGDYMSAASFLGIAGLVYGSGYDGLIYAIG
                     FLVGWPLVLLLIAEPLRNLGRYTFADVASFRLQQSPIRILAASGSLITVAFYLIAQMV
                     GAGKLVELLFGLPYWVAVVTVGVLMTLYVVFGGMLATTWVQLIKAVLLLSGASLIAFL
                     VMAQFGFSFEQLFASAVGVHKKGAAIMGPSALVTSPVSAISAISLGVALMFGTAGLPH
                     ILMRFFTVRDAVNARKSVVYATGFIGYFYILTFIIGFGAIVLLIQHPEFFKDGALIGG
                     TNMVAIHLSNALGGELLMGFISAVAFATILAVVAGLTLAGSTAICHDLYAKVVLKGVH
                     NEKKEMLASKIATVGLGVMAVGLGIIFEKQNVAFMVGLAFCVAASCNFPILLLSMYWK
                     GLTTRGALWGGGSGLVLALVLVIIGPTVWVDALGNKEAIIPIKNPALFSVSLAFFMCW
                     LMSITDKSAQASAEKAQFDDQNVRAQTGLGIHVSGSH"
     gene            complement(3217..3540)
                     /locus_tag="PKZ52_03525"
     CDS             complement(3217..3540)
                     /locus_tag="PKZ52_03525"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNG58870.1"
                     /translation="MSPSPQSPNASNILEHPLYIQLLKTRKKITRPLTIAGLLSYYGL
                     IVAGAFYPKELGQPLFEGSVTSWGIVLGFAVIIGCLIITSIYVYYANKYIEPILKQLK
                     EQVKP"
     gene            complement(4349..4996)
                     /locus_tag="PKZ52_03530"
     CDS             complement(4349..4996)
                     /locus_tag="PKZ52_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNG58871.1"
                     /translation="MDDGLKQRIVGALVLLALLVIFLPVLFDRNRVEPVDPTSQIPPP
                     VSVPAAPINAPQVANVAEADMAPPPAQMLVPAENLAEKPEQIAAQTAKLEAAKTAKND
                     AAKADSKNTLKLTKSESVKPVLEAPKLNNKGSAEAWLLQAASYKTAAQAKKLRDDLAA
                     KGFNAYTRELTTPKGLVVRVFIGPKFDKATALEQKKRIDELFKLNVLLLPYTPSN"
     gene            complement(4989..6305)
                     /locus_tag="PKZ52_03535"
     CDS             complement(4989..6305)
                     /locus_tag="PKZ52_03535"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNG58872.1"
                     /translation="MPSLPLTALVPRFTQLADWLAWQETLHPKVIDLGLERVRAVAAR
                     LDAELGGGLLAPKARTVLIAGTNGKGSCVASLTALVGAAGGKVGCYTSPHLLRYNERI
                     AINGQEVSDADLCRAFAAIDRARGEISLSYFEFGTLAAYWLMAQAELDVWVIEVGLGG
                     RLDATNILDADIAIITSIALDHEAFLGNTRELIAAEKFGIVRPQSCLICAELDPPESL
                     KQGAARLNCPAAWAGRDFSWSQHEDGAVDIRLPPPGTLQVPHLQLPIPSVAAALMAFA
                     QLGYALDPAVLVAVLSGLRLAGRWQRFVWQGVDILLDVAHNPASTQALAAYLAREGGA
                     FVLLMGMMADKNIAASLKPLNKAAHLIAVGLPTPRAAAAAALAASAQALGFNTSAYVN
                     LADALPQALELCRSHSARLVICGSFYTLADAYPALAELGACEVQCG"
     gene            complement(6305..7168)
                     /gene="accD"
                     /locus_tag="PKZ52_03540"
     CDS             complement(6305..7168)
                     /gene="accD"
                     /locus_tag="PKZ52_03540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNG58873.1"
                     /translation="MSWLDKIVPAVRAVRAGTSKVPEGLWKKCGKCDAVLYRPELEKN
                     LDVCPKCDHHMRISARRRLDIFLDPQGREELATDVLPIDRLKFKDIKRYKERLTAAQK
                     ETGEKDALIAMKGTLKGMPVVAVAFEFAFHGGSMGYVVGERFTRAAQVALKERCPLVC
                     FSATGGARMQEALISLMQMAKTSAVIERMKQAGVPYISIMTDPVYGGVSASLALLGDI
                     NAAEPGARAGFAGQAIIEQTIRQKLPKGFQRAEFLLEHGAIDMIIHRKDMVERVGNLL
                     AKFTRRAPLSA"
     gene            complement(7207..8013)
                     /gene="trpA"
                     /locus_tag="PKZ52_03545"
     CDS             complement(7207..8013)
                     /gene="trpA"
                     /locus_tag="PKZ52_03545"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNG58874.1"
                     /translation="MNRINQTLAKLNGEGRKALATYLVCGDPKPAFTLTAMHALVAAG
                     ADIIELGVPFSDPMAEGPTIQKAHERALAFNTSLRTSLEVVTAFRSTNPHTPVVLMGY
                     ANPVERMGYDTFVQAAVEAGVDGLLTVDLPPEEAEDFNTKMRAAGLETIYLVAPTTAP
                     ERVRAITAAATGFVYYVSLKGVTGAGNLDVTDVASKLAGIRSTTQTPVLVGFGIKDAA
                     SAKAVAANADGVVVGSALVELMAVPSSDDALLQALTAKVRDIRQGLDSLS"
     gene            complement(8017..9234)
                     /gene="trpB"
                     /locus_tag="PKZ52_03550"
     CDS             complement(8017..9234)
                     /gene="trpB"
                     /locus_tag="PKZ52_03550"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNG58875.1"
                     /translation="MSSHIPADFSAVPDARGHFGPYGGRFVSETLIYALDQLEAIYQR
                     LKNDPQFIAEFDRDMAHYVGRPSPLYFAESWTRKVGGAQLFLKREDLNHTGAHKINNT
                     IGQALLAKHTGKQRVIAETGAGQHGVATATVAARLGLECVVYMGEEDVRRQSLNVYRM
                     KLLGATVVPVTSGSKTLKDAMNEAMRDWVTNVDSTFYIIGTAAGPHPYPQLVRDFQSI
                     IGREARAQSLAQTGRLPDALVACVGGGSNAIGLFHPFLSDSSVAMYGVEAGGLGIDTG
                     KHAAPLTAGIPGVLHGNRTYLMEDENGQIIETHSVSAGLDYPGVGPEHAWLKDLGRVN
                     YVAVTDDEAMAGFRRLTRAEGIMPALESSHAIAYGEKLAKTLPEDAIVIVNLSGRGDK
                     DILTVAKLDGIEV"
     gene            complement(9275..9895)
                     /locus_tag="PKZ52_03555"
     CDS             complement(9275..9895)
                     /locus_tag="PKZ52_03555"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNG58876.1"
                     /translation="MAVRIKICGITRLEDARALEQLGADAQGLVFYAKSPRYLADLAQ
                     ARALADAAGPFVTSVGLFVNPAASWVNQVLSQVPLQLLQFHGDESNDFCAAFARPFIK
                     ALRMKPGLDLAAEMGQYPDAQGFLLDTYKPGVPGGTGEAFDWSLSPKTCPKAWVLAGG
                     LTPDTVAAALSQTAPYGVDVSGGVESAPGVKCHTKLHDFIQRARGQ"
     gene            complement(10064..10930)
                     /gene="truA"
                     /locus_tag="PKZ52_03560"
     CDS             complement(10064..10930)
                     /gene="truA"
                     /locus_tag="PKZ52_03560"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNG58877.1"
                     /translation="MSNVARYTPGCELKPGMTPPENAQRWALIVEYLGRDFNGFARQA
                     TAPNTVQQRLETALSKVACEEIQIICAGRTDAGVHATAQVVHFDTTRPRPSKAWVLGA
                     NSYLPASIRVHQALPVSWHFHARFSALARTYRYLLASALVPSATLADQVSWTRFALDH
                     TAMQQACGYFMGEQDFTSFRTVQCQAKSPVRRIEHIQLQAVNGLLVLEITANAFLHHM
                     VRNIMGSLIEVGRGARPPEWIAEVLAARDRSVAAPTAQPGGLYLVDVAYPPEFAIPKL
                     AKGPLLLPQQLS"
BASE COUNT         2610 a         3076 c         2977 g         2422 t
ORIGIN      
        1 ccgccaccgc catagtgcgc gagcgagaac agggcaatat ggaattttta attaccacgc
       61 ctattcaccc ggtggaatta atgctgggta aaattattcc ctatgtactg gccgggttgg
      121 tgcaatcatc aattattctc agcctcggcc acagcttatt tggcgtaccg attaatggca
      181 gtttgctggc tctgtttata gtggtgatta cctttatttt agccagcctc accctgggct
      241 taattattag caccctggtg caaacccagt tacaagccat gcagatgaca gtatttattt
      301 tattgccgtc tattttgtta tccgggttta tgttccccta cgaagctatg ccggtggtag
      361 cccagtatgt agcagaacta ttacccgcca cccattttat gcgctgcatt cgcgccgtgg
      421 tgctgcgctc ggcaagcctt ggcgaccttg catttgattt aagctggtta gtgttatttt
      481 gttttgccgg tttgttggtg gcatccctgc ggtttaaaaa gcgtttggat taatcaccac
      541 acaatcaagc gccgccgcaa ataaaaaaac cggcctgggg ccggtttttg caaatctagc
      601 agagtattac tttaaatttt ccgctctttg cgcacatatt tcatgatatt tataaacgta
      661 tccgtccaga tagtgtcttt gtgggccgcc agataactta ataactcttc atgggcctga
      721 ttagaaaccg ttaaatagtc gccattaata ccgtggaagg taaaattagc catggtgcct
      781 tttgccaccg cctgctccac tatggcaatt aattctttgc cggtgatatt agacggcagg
      841 gttacactga cataggcagg atcgagcgta ttcatatcct cagtcacacc gctgccgccg
      901 gtatttttaa tcccgacaaa taaagtgcgc accgcctcta cataattttc gccattggcg
      961 acttttttat ctaaacaggg ggtggtcatg gtgcgttcgg ttttgccatc gagcatgaat
     1021 aaaaaggtat tggccaattt aacctggtcc accatttgat ccaggctgat tttatccaga
     1081 ttgttttttt ccaacacaaa atcccgcccc ggctgggatt tagagcactg gtgaaacagc
     1141 gtgtgattgc ccagctcatg gccctgctga gccaccttgc gccaagcttc taggcgggtt
     1201 tgcaacgcag gcgccgcttc tatcaaataa aaagtgccct taatattaaa tttatttaaa
     1261 gacggcactg cattatctaa ctgagaatcc agcgtatcgt cataagacag gctaactgcc
     1321 gcctgatatt tcttcggcca tttaaattcg cccgcccagg tgctgctcat ccaactcatg
     1381 gcggccagcg ccgccaccca gggcaggcat ttgcccaatc ggtaagacgc ctgcgtcaag
     1441 gttcgtgtgg ttaaattact cattaattat ctcccgcata ggatggtgat gggcaaaaac
     1501 gcgctgtgta tcactatagt gcccaacacc caaaacacaa aaaaaacggc gcctaaggcg
     1561 ccgctttact tgccatcagc ccctaatggc taccgctgac atgtatgccc aagcccgttt
     1621 gcgcgcgcac attttggtcg tcaaactgcg ctttctccgc acttgcctgg gccgatttat
     1681 cggtgatgga catcagccag cacataaaaa acgccaagct cacggaaaac aacgcggggt
     1741 ttttaatagg aataatggct tctttattac ccagtgcatc cacccaaacc gtcggcccaa
     1801 taatcactaa caccagcgcc aacaccaatc cgctgccgcc gccccagagg gcgccacggg
     1861 tggttaagcc cttccaatac atgcttaata acagaatggg gaaattacag ctggccgcca
     1921 cgcaaaaggc taagcccacc ataaaggcca cgttttgttt ctcaaaaata atgcccaggc
     1981 ccaccgccat aacacctagg cccaccgtgg caattttaga ggcgagcatt tcttttttct
     2041 cgttgtgcac acccttgagt actaccttgg cgtagaggtc gtggcaaatg gccgtggagc
     2101 ccgccaaagt aaggcccgct accacagcca aaatagtggc gaaggctact gcggaaataa
     2161 agcccattaa taattcaccc cccaaggcat tggataggtg aatggccacc atattagtgc
     2221 cgccgattaa ggcgccgtct ttaaaaaatt ccgggtgctg aatcaataag acgatggcgc
     2281 caaagccgat gataaaggtg agaatataaa aatagccgat aaagcccgtg gcatatacca
     2341 ccgatttacg ggcgttcacc gcatcgcgca cagtaaaaaa acgcatcaaa atatgcggca
     2401 accctgcggt gccgaacatt aaggccaccc ccaaggagat agcggaaata gccgataccg
     2461 ggctggttac cagggcgctg gggcccataa tggcggcacc ttttttatgc acacctacgg
     2521 cggaggcaaa taattgttca aaactaaagc caaattgcgc cataactaaa aaagcaatta
     2581 aggacgcgcc gcttaacaat aacaccgctt taattaactg cacccaggtg gtggccaaca
     2641 taccgccaaa cactacatac agcgtcatta acacacccac ggttaccacc gctacccaat
     2701 agggcaaacc aaataacaac tcgactaatt taccggcccc caccatttgt gcaatcagat
     2761 aaaacgccac cgtaattaac gagccgctgg cagcgagaat gcgaataggc gattgttgca
     2821 aacggaacga ggccacatcg gcaaaggtgt aacgacctaa attgcgcaag ggttcagcaa
     2881 taagcagtaa taccagcggc caaccaacta aaaaacctat ggcgtaaata agaccgtcat
     2941 aacccgagcc gtacactaag cctgcgatac cgagaaatga cgcggcggac atataatcgc
     3001 ccgcaatagc caaaccattt tgaaacccgg taataccgcc accggccgtg taaaaatccg
     3061 ccgcgctttt agtccggttt ttggcccagt aggtaatccc tagggtaatg gctacaaaca
     3121 caacaaacat aataatggcc agggtgtgat ctggcgcacc gccttcggca taggcccata
     3181 atgggcacag gctaaataat aaagcgagca ctaatttcat ggcttcacct gctcttttaa
     3241 ttgtttcaat ataggctcga tgtatttatt ggcgtaatac acataaatgg aggtaataat
     3301 taaacagcca ataatgacag caaaaccgag cacaattccc caggaggtca ccgaaccttc
     3361 aaacagtggt tggccgagtt ctttagggta aaaggctcct gccacaatta aaccgtaata
     3421 ggaaagtaag cccgcaatgg ttaatggcct ggttattttt ttgcgagttt ttaacagctg
     3481 aatatagagc ggatgctcca agatgttcga tgcgttaggc gactgcggtg atggcgacat
     3541 aaaaccctca ggggctagca ggtggatggg ataagcggca aacacaaccg cgcgctgcag
     3601 cttacacgtt aagttttcag ctgtcagcag accctattcg caaaacccat caatacaaca
     3661 aggccacagg tagcaagcgc aggcacgcga agaaatgtaa caaatagtgg gcggacactg
     3721 actgagcact taaacctaac aaccttagtt aacagcggaa attctggtta aatctgaatt
     3781 cgtactagcg cgcagatagg cccatagatc tttgtagcgc tattttgcag gtaaaaattg
     3841 tcagaggatt tacgaggggc ttagagtcaa atagaaatta tttcccggcc actaaaagtc
     3901 gttacaacta taaaacctgt aacctcagtt gatccgcagc gcggaatctt cattttttaa
     3961 tgtgaaaatt attcgcagct tttttgtgta gctagcggtg attcgcgcat ccacgggaag
     4021 ccattaagta ggtgctttag ttctgcgggc atttcatact acgcccgcac ttgtgaatgc
     4081 aatgtcattt agttaaggat taactgccat ttctcaagcc tggatggagc ttggcaatat
     4141 aggcgagcca aatccttaac ctaaggattt ggctcggcgg gattgatgtc ggaagtcgct
     4201 gcgctcttcc aacctacgcc tgaacgggtt tatgccttaa tttaagacgc cggggataga
     4261 tatgggctta ttaaattggc ggcaaaatag atcgtctctg ctctattttg ccgccaattt
     4321 aataagcgat ggatgtgcag aggggtaatt aattactggg ggtataaggc agcagcagca
     4381 cattgagttt aaataactca tcgatgcgtt ttttctgctc tagggcggtg gccttatcaa
     4441 atttagggcc gataaaaacg cgcaccacca ggcctttggg ggtagttaat tctcgcgtat
     4501 aggcattaaa acccttagca gctaagtcat cccgcaattt ttttgcttgg gccgccgttt
     4561 tgtagctggc cgcttgtaat aaccaggctt cggcagagcc tttgttattg agtttagggg
     4621 cttctaaaac cggttttaca ctttcgcttt tggttaattt cagggtgttt ttgctgtcgg
     4681 ctttagcggc gtcatttttg gccgttttgg ccgcttcgag tttggctgtt tgggcggcga
     4741 tctgctcggg cttttccgct agattttctg ccggtaccag catctgggcg ggcggtggtg
     4801 ccatgtccgc ttccgccaca ttcgccacct ggggcgcatt aataggcgcc gccggaacgc
     4861 ttaccggcgg ggggatttga ctggtggggt ccaccggttc cacccggttg cgatcgaaca
     4921 atacgggcaa aaaaatcacc aataacgcca ataacaccag tgcgcccact atgcgctgct
     4981 ttagcccgtc atccacactg cacctcacag gcacctagct cggccagtgc cgggtaggca
     5041 tcggctaggg tgtagaagga gccgcagatc accaagcggg cactatggct gcggcacagc
     5101 tccaacgctt ggggcagtgc atcggcaagg ttcacatagg cgctggtgtt aaaacctaag
     5161 gcctgggcgc tagctgccag ggcagcggca gcggcagccc gaggggttgg caggccgacg
     5221 gcaattaagt gggcagcctt attgaggggt tttaagctag cggcgatatt tttatcggcc
     5281 atcatgccca tcagcaacac aaacgctccc ccctcacggg ctaggtaagc ggccagggct
     5341 tgggtagaag ccgggttgtg ggccacatcc agcagaatat caacgccctg ccaaacaaac
     5401 cgctgccagc gcccggccag gcgcaacccg ctcaacaccg ccaccagcac agccggatca
     5461 agggcatatc ccagctgggc aaaagccatt agggccgcag ccacgctcgg tatgggcaac
     5521 tgtaaatggg gtacctgcag ggtgccgggc gggggtaaac gaatgtctac tgccccgtcc
     5581 tcgtgctgtg accaggaaaa atcgcgcccc gcccaggcgg ccgggcaatt aagccgcgct
     5641 gcgccctgct taaggctctc cggtgggtct aactcggcgc aaatcaaaca actctgcggg
     5701 cggactatgc cgaatttttc tgcggcgatt agctcccgcg tattgcccaa aaaggcctcg
     5761 tgatccaggg cgatggaggt gatgatggca atgtcggcat cgagaatatt ggtggcatcc
     5821 aagcggccac ccaaacccac ttcgatcacc caaacatcca actcggcctg ggccatcagc
     5881 caataggctg ccagggtgcc aaattcaaaa taactcaggc tgatctcgcc gcgggcgcgg
     5941 tcgatggcgg cgaaggcgcg gcacagatcg gcatcgctca cctcctggcc gttgatggcg
     6001 atgcgctcgt tgtagcgcag cagatggggt gaggtataac acccgacctt gccgcccgcc
     6061 gcgcccacca gcgcagtaag actggccaca caggagccct tgccgttagt cccggcgatt
     6121 agcacggtac gggccttagg agccagcagg ccgccgccca attcggcatc cagccttgcg
     6181 gccacagcgc gcacccgctc cagacccaaa tcaatcacct tggggtggag ggtctcctgc
     6241 caggccagcc aatcggccag ttgggtaaaa cgcggcacca atgccgttaa gggcaggctc
     6301 ggcattaagc actcaaaggc gcgcggcggg tgaatttagc cagcaagttg cctacccgct
     6361 ccaccatatc cttgcggtga ataatcatgt cgatggcgcc gtgctccaac aaaaattctg
     6421 cgcgttgaaa gcctttaggt agcttctgcc gaatggtttg ttcgatgatg gcttgaccgg
     6481 caaaacccgc ccgggcgccc ggctccgcag cgttgatatc ccccaacagg gcaaggcttg
     6541 ccgatacacc accgtatacc gggtcggtca tgatcgagat atagggcaca ccggcctgtt
     6601 tcatgcgctc gatcaccgcg ctggttttgg ccatttgcat gagggagatc agcgcctcct
     6661 gcatacgggc gccgccggtg gcggaaaaac acaccagggg gcagcgctcc tttagcgcca
     6721 cttgggcggc gcgggtgaac cgctcgccca ccacgtatcc catggagcca ccgtggaagg
     6781 cgaactcaaa ggctaccgcc actacgggca tacctttaag ggtgcctttc atggcgatta
     6841 aggcgtcttt ttcgccggtt tctttttggg ccgccgtcag gcgctctttg tagcgtttga
     6901 tatctttaaa tttaagccga tcgatgggca acacgtcggt tgccaactcc tcccggccct
     6961 gggggtcgag aaaaatatcc aggcgcctac gggcgctgat gcgcatgtgg tggtcgcact
     7021 tggggcagac gtcgaggttt ttttccagct ccgggcggta caacaccgca tcgcacttgc
     7081 cacatttttt ccacaggcct tccggcactt tgctggtgcc tgcgcgcacc gcgcgtacgg
     7141 cgggcacaat tttatctaac caactcatat cgttctctat attctcaggt ggcaaaggcg
     7201 tgtccattag gacaggctat caaggccttg gcggatgtcg cgcaccttgg cggtaagcgc
     7261 ctgcaacaaa gcgtcatcac tactggggac ggccatcaac tcaaccaggg cgctgcccac
     7321 caccacacca tcggcattgg cggcaacggc tttggcggag gcggcgtctt taataccaaa
     7381 gcccaccaac acgggggttt gggtggtgct gcgaatgccc gctaatttac tcgcgacgtc
     7441 agtcacatcc aaattgcccg cgcccgttac gcctttgagt gacacgtaat agacgaaccc
     7501 tgtagccgcc gccgtaatcg ctcgcacccg ctcaggcgca gtggtgggtg ccaccaaata
     7561 aatggtttcc aggcccgccg cccgcatttt tgtattgaag tcttcggcct cttccggcgg
     7621 caaatccacc gttaacaggc catctacgcc agcttcgaca gccgcttgca caaaggtgtc
     7681 gtagcccatg cgctcaaccg ggttggcata gcccatcagc accacggggg tgtgagggtt
     7741 ggtgctgcga aatgcggtta ccacctccag gctggtgcgc aggctagtgt taaaggccag
     7801 ggcgcgctca tgggcttttt gaatggtggg gccttctgcc atagggtcgg aaaagggtac
     7861 gcccagttca atgatatctg cacccgccgc caccagggcg tgcatggcgg tgagggtaaa
     7921 ggcgggtttg ggatcgccgc agaccagata agtggcgagc gctttgcgcc cctcgccatt
     7981 aagcttagcg agggtttggt tgatacgatt catgctttaa acctcaatgc cgtcgagctt
     8041 ggcgacggtc aatatatctt tatcgccgcg gccagagagg ttgacgatca ctatggcgtc
     8101 ctccggcagc gttttagcga gtttttcgcc ataggcgatg gcgtggctgg attcaagggc
     8161 gggcataatg ccttcagcgc gggttaagcg cctgaagcct gccatggctt cgtcgtccgt
     8221 caccgccaca taattcacgc ggccgagatc ttttaaccag gcgtgttcag ggccaacgcc
     8281 ggggtaatct aagcctgccg acaccgagtg agtctcgata atctggccgt tttcatcttc
     8341 catcaaataa gtgcggttgc cgtgcagcac accgggaatt ccggcggtga ggggcgcggc
     8401 gtgtttgccg gtatcaatac ccaggccacc ggcctcaacg ccatacatgg ccacgctgct
     8461 gtcgcttaag aagggatgga acaggccgat ggcgttggag ccaccgccca cacaggccac
     8521 cagggcatcc ggcaggcgac cggtttgcgc caggctctgg gcgcgggcct cgcgaccgat
     8581 aatggattga aaatcccgta ccaactgcgg ataaggatgg gggcctgcgg cggtgccgat
     8641 gatgtaaaag gtgctatcca cgttggtcac ccaatcgcgc atggcttcat tcatggcgtc
     8701 tttgagggtt ttagagccgg aggtgaccgg caccactgtg gcgccgagaa gcttcatgcg
     8761 gtacacattc agggactgac ggcgcacgtc ttcctccccc atgtagacca cacattcaag
     8821 ccccaaacga gccgccaccg tagcagtggc cacaccgtgt tggcccgcgc ccgtctcggc
     8881 gataacccgc tgtttgccgg tgtgcttagc cagcagggcc tggccgatag tgttattaat
     8941 tttatgggcg ccggtgtggt ttaaatcctc gcgttttagg aataactgcg cgccgcccac
     9001 cttgcgggtc cagctctcgg cgaaatacag gggcgaaggg cggcctacgt aatgggccat
     9061 atcgcggtcg aattccgcga taaattgcgg gtcgtttttc aagcgctggt agatggcctc
     9121 taactggtcc agcgcgtaaa taagcgtttc cgacacgaag cggccgccgt aggggccaaa
     9181 atgaccgcgg gcatccggca cggcggagaa atctgcaggt atgtgtgagc tcacaagtcg
     9241 attccgtagt aaagaaatag gttcggcgaa aggcttattg cccccgcgcc cgttgaataa
     9301 aatcgtgcaa tttagtgtgg catttaaccc ccggcgccga ctccaccccg ccgctgacat
     9361 ccaccccata gggtgcagtc tgagagaggg ctgcggccac cgtgtcaggg gtgaggccac
     9421 ccgctaacac ccaggccttc gggcaggtct tagggcttaa gctccagtca aacgcctcac
     9481 cagtgccgcc gggcaccccc ggtttgtagg tatccagcag aaagccctgg gcgtccgggt
     9541 attgccccat ctcagcggcc aggtctaagc cgggtttcat acgcaaggct ttgataaaag
     9601 gtcgagcaaa ggcggcgcag aagtcgttgc tttcatcgcc gtggaactgc agcagctgca
     9661 atggcacctg gctgagcacc tgattcaccc aactggcggc agggttaaca aacaggccca
     9721 cgctggtaac aaaaggcccg gcggcgtccg caagcgcccg cgcctgggcc aaatcggcga
     9781 gataacgggg gctcttggcg taaaacacca ggccttgggc atccgcaccc agctgttcga
     9841 gggcgcgggc gtcttctagg cgagtgatgc cgcaaatttt aattctgact gccaaatcga
     9901 gcctcacagc aaaaccgaac cctaagcgca ggtgcgccgc aacatccggg caaaaagtac
     9961 taaagggcgg cgatagtacc agacttagcc taaacctaaa aatctctgtt taacgccgcc
    10021 actgcggccc gctatcgccc gcgtgcggta ctacaggccg ccctcagctg agctgctgcg
    10081 gcagtagtag tgggcctttg gcgagcttag ggatggcaaa ctcgggcgga taggcgacat
    10141 ccaccaaata aagcccgccg ggctgggcgg tgggtgccgc cactgagcgg tcgcgggcgg
    10201 ccagcacctc ggcaatccac tccggcgggc gggcgccgcg tcccacctca ataaggctgc
    10261 ccatgatgtt gcgcaccata tggtgcaaaa aggcattggc ggtaatttct aacaccaaca
    10321 ggccgttcac agcctgcagc tgaatatgct cgatgcggcg caccgggctt ttagcctggc
    10381 actgcacagt gcgaaacgag gtgaaatcct gctcccccat aaagtagcca caggcctgct
    10441 gcatggcggt gtgatccaag gcaaagcggg tccaactcac ctgatcggcc aaggtggcgc
    10501 tgggaaccag ggctgatgcc aataaatacc ggtaggtgcg ggccagggcg gaaaagcgcg
    10561 cgtggaagtg ccagctcacc ggcagggcct ggtgcacgcg aatgctggcg ggcaaataac
    10621 tattggcccc cagcacccag gctttactgg gccttgggcg ggtggtatca aagtgcacca
    10681 cctgggcagt ggcgtgcact cccgcatccg tgcgcccggc acagattatc tgaatttctt
    10741 cacaggccac cttactcaag gcggtttcca aacgctgctg aaccgtattg ggcgcggtgg
    10801 cctggcgggc aaaaccgtta aagtcgcggc ccagatattc cacaatcaat gcccagcgct
    10861 gggcgttttc cggtggcgtc atgccaggct taagttcgca gccgggtgta tagcgcgcga
    10921 cgttactcac ctctgcctcc cggcggtttt tataggtaca acttcaggca aactcgacgg
    10981 caattcaccg ggtacataag tatttagcaa cataaagacg cacataaaaa aggccccgca
    11041 gcaggctgcg gggccttgat aagatagtga agtagttagc tgatg
//
LOCUS       DAOQGS010000058        11031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2757_length_11031_cov_4.109512, whole genome shotgun sequence.
ACCESSION   DAOQGS010000058 DAOQGS010000000
VERSION     DAOQGS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11031
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_11031_cov_4.109512"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1845)
                     /gene="ubiB"
                     /locus_tag="PKZ52_03565"
     CDS             complement(223..1845)
                     /gene="ubiB"
                     /locus_tag="PKZ52_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNG58878.1"
                     /translation="MFESSQRLLTILRVFARYQLLELLPEEYQKPWLKLLFACFKRDA
                     LKLPRGERLRLACQDLGPIFVKFGQLLSTRPDLIPPDICLSLNQLQDRVAPFPSEQFV
                     ALVEEALGQPVAVLFSEFSLEPLASASIAQVHCARLHSGEQVVIKAVRPGIESTIHSD
                     LSLMLVLARWIAALNSDGKRLKPVEVVEEYRNTILDELDLMREAANAAQLKRNFTPPS
                     QLYVPKMYWDYCRTNVLVMERIWGVQVTDIKGITAAGVDLKTLAELGVEIFFTQVFDH
                     NFFHADMHPGNVYVSTNNPRSPHYIALDMAIMGSLTREDQYYLARNLLAMFRRDYRQV
                     AELHVVSNWVPKNTSVAAFESAIRSVCEPIFAKPLKDISFGQALISLFQTARRFNMEI
                     QPQLVLLQKTLLNIEGLGRQLYPELDLWATAHPFLERWVQRRYHPKTLWKELKRQAPD
                     WLEQLPTLPQLSLDALHSAKTLAAIAPDVQALARSLEQRERRRPWRAAAAVLCALSFT
                     ALALRFGGGFGLGDWLVLCLGFLGVMLAIISR"
     gene            complement(1838..2473)
                     /locus_tag="PKZ52_03570"
     CDS             complement(1838..2473)
                     /locus_tag="PKZ52_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNG58879.1"
                     /translation="MSLPLIGAGLRSALEAALNSALSLDPASQRRLEDLAGKSVQLEI
                     TDLRLNWCLHLNYPLLLLEAGAETAANSRLSGKLQDFVGLALKDSASLSHSGVSHAGD
                     IQLLNTCIQLFKNLELDWAGVVSEHLGPLPGALVDQLYEACGTLKQTLAKLPPFLGDY
                     VQNELQLVPSKPQLDLFTQDIHELRSRADRLTARIERLDARLDQERQKPHV"
     gene            complement(2470..3219)
                     /gene="ubiE"
                     /locus_tag="PKZ52_03575"
     CDS             complement(2470..3219)
                     /gene="ubiE"
                     /locus_tag="PKZ52_03575"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNG58880.1"
                     /translation="MERDEITHFGFQSVKAGEKAAKVASVFHSVAAKYDVMNDVMSLG
                     IHRLWKRLTIEAAGARAGHKILDIAGGTGDLTAQFARIVGPGGEVVLADINASMLGVG
                     RDKLIDQGLVNNLSFVQADAQHLPFPDNYFDCITIAFGLRNVTDKDLALASMFRVLKP
                     GGKLLVLEFSKPHNPLLAKAYDQYSFHLLPLMGKLVANDADSYRYLAESIRMHPDQAT
                     LKTMVLNAGFARCEVHNFTGGIVALHKGFKA"
     gene            complement(3355..3966)
                     /locus_tag="PKZ52_03580"
     CDS             complement(3355..3966)
                     /locus_tag="PKZ52_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNG58881.1"
                     /translation="MKNLVAVMTALALTSQASASVLTEDFSDVPGWESNWFGTQSNAK
                     NYYGVKGFGNATRNSNQDGLWIDDNDGISHESDLTILFNDVFAASLTSFSLDLASYIT
                     DTNIVFFDALGNNLATQAVTPTYGAMQLPGLYQSFTVSSATGIGGFSLINFANTSVEG
                     NVSIDNLLAITNDAPSVSVPEPTSLILLGLGALGLIAQRRRLV"
     gene            4209..6443
                     /gene="flhA"
                     /locus_tag="PKZ52_03585"
     CDS             4209..6443
                     /gene="flhA"
                     /locus_tag="PKZ52_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNG58882.1"
                     /translation="MAESIFTHLRGLPSTMTRDSALAGFRGLWHGNLAVPILLLMLLG
                     MMILPMPPFLLDAFFTFNIALSIVVLLVSIYSLRPLDFAVFPTILLVATLLRLALNVA
                     STRVVLLEGHKGGDAAGKVIEAFGEVVIGGNYAVGLVVFIILMIINFVVVTKGAGRIS
                     EVSARFTLDAMPGKQMAIDADLNAGLIDQDEARKRRVEVAAEADFYGSMDGASKFVRG
                     DSIAGILILFINIIGGLIVGLVQHHLEFQDALQKYILLTIGDGLVAQIPSLLLATASA
                     IIVTRVNTTQDMGSQVFSQMFASPKALGVSAVILFIMGSIPGMPHVAFIGLGMVCAGL
                     AYFIHFRSQQAEVVEEGRPQGMPRPAPAPMGGSGNAPMNTGIAALEHRASAQSETVEV
                     SWDDVTAVDILGLEVGYRLIPLVDKNQRGELLGRIKGVRKKMSQDLGFLVPPVHIRDN
                     LELLPSAYRITLMGVSLGEGEVFPDRHMAINPGQVFGPLPGAKTQDPAFGLEAIWIDE
                     SLKDQAQTLGYTVVDASTVVATHLNQIIQKHTHELLGHEEVQKWLDLLAEKSPKLVEE
                     LVPGTISLNTLLKVLQNLLREQVAIRDRRSIAEALAASGDKSQDIATLVGHARVALAR
                     QIVQNIVGSDLSIPCITLEPSLEHLLLQSYQQAQKAGADGSSFIEPRLAERLNNSLVK
                     AAQKQEAQGKPQVLLVAAPIRAMLAGFVRYNLPDMQVLAYNEVPDNKQITIEATVGAE
                     AKPQ"
     gene            6463..8043
                     /gene="flhF"
                     /locus_tag="PKZ52_03590"
     CDS             6463..8043
                     /gene="flhF"
                     /locus_tag="PKZ52_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNG58883.1"
                     /translation="MTVKRFVAPDMRRALDLVRQEMGPEAIILSSKRIKEGVEILTAD
                     QLDIPTRGYDARKQFGEQFDSDLDRPLISDRDWQPDTRLPAKPQAPLAPPVNSQAERA
                     VALAKEIEAARARMLAAKKSAQAANRPIGELLAGQRAEPSLPPAAASETVARFDKPNR
                     IEPRVSGLSPAETADPFEFDFGFDQLVAVKPAPLQDEARPDGQLANLAAQVGQLQERA
                     PVARSDDTRLNALQAEIADMRSLLERHLRHWPASTEEFTSKPSAGHYALGQQLQRLGI
                     GAELVERLCQHCANQPRLNLAWREALNELVARLPVQSTDPIDQGGVFAFVGPTGVGKT
                     TTIAKLAARYVLAHGQGKVALVTTDTYRVGAQDQLRSLGRILNVPVRIIDQERALPSV
                     LASLKAYPLILIDTAGFRQGDPLLREQEAILAACPSVQRVLVMAGNSQVQTLKASAHA
                     YKARRLAGCVLTKIDETASLGEAISVVIQQAIPLLYTTFGQEIPKDIAVASAQSLVAK
                     AVNLAKQESAQESSVGLS"
     gene            8149..8976
                     /locus_tag="PKZ52_03595"
     CDS             8149..8976
                     /locus_tag="PKZ52_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNG58884.1"
                     /translation="MMASRPVKVVAITGGKGGVGKTNLSVNLSIALAEMRRRVVLLDA
                     DLGLANVDVLLGLQARFTLADVLSGERDLREVMLNGPAGLKIVPASSGLQNMVKLSAQ
                     EHAAIVHAFSELSDQIDVLVIDTAAGISDTVTSFVRAAQEVVIVVCDEPSSITDAYAL
                     MKLLNTEHGITRFKVIANMTRTLQEGSALFNKLNAVCERFLDASLQYLGPVPFDENVR
                     KAVQKRKALLEFAPRCKASQAIRFIAQRIDQWPVQNSARGHLEFFFERLVASASGGM"
     gene            8994..9710
                     /locus_tag="PKZ52_03600"
     CDS             8994..9710
                     /locus_tag="PKZ52_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNG58885.1"
                     /translation="MRFAAVNQEPGVDIEAFAPLVKKIAYHLLARMPASVQLDDLIQA
                     GMLGLIEAAQKYDDSKGASFETYAGIRIKGAMVDEMRRGDWVPRSVHRNARKISQALA
                     SLEASLGREACDAEVAAHLEMSIEEYQALLADTQGSRLFSYNEDIDDEESCASADDSS
                     APLQDPAKSVAQTHLQSSLAAAIAELPEREQLVLSLYYEQEMNLKEIGLVLGVSESRV
                     SQIHSQAALRLKGKLSGWAR"
     gene            10088..10351
                     /locus_tag="PKZ52_03605"
     CDS             10088..10351
                     /locus_tag="PKZ52_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58886.1"
                     /translation="MKRLGFCSWVVLVGLISGNAMAAQSLSEAKKIVADYQILREACS
                     DATGAKRLECMARLSSASDSYREAKTFVMASSGSTQLKIAKAH"
     gene            10699..>11031
                     /locus_tag="PKZ52_03610"
     CDS             10699..>11031
                     /locus_tag="PKZ52_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNG58887.1"
                     /translation="MYVYDEHDSKIIHERVAQFRFQTQRFLDGKLKPEEFLPLRLQNG
                     LYVQRLAPMLRIAVPYGMLNAVQLRKLAYICRHYDKGYCHATTRQNIQLNWPQLEEVP
                     DILAELAQV"
BASE COUNT         2376 a         3148 c         3161 g         2346 t
ORIGIN      
        1 actggcaata ttgggggagt ccatatatgg aagagcgcag cgacttccga catccaaccg
       61 cccgcataaa ctctgggcaa ggcaaggcaa ggcaaggcaa ggcaaggcaa gcagggactt
      121 ggcgggatgt cggaagtcgc tgcgctcctc caacctacgc tttggcaggc gttttcctta
      181 acgccgtaaa tttgcacgca agcgcaaacc tcgccgcaat tcttaccggc taataatcgc
      241 caacatcaca cccaaaaacc ccaggcacag caccaaccaa tcacccaaac caaagccgcc
      301 gccaaaacgc agggcgaggg cggtgaagct cagcgcacac aacacagcag cggcggcccg
      361 ccagggccgt ctgcgttcgc gctgttcaag gcttctggcc agggcctgca catccggcgc
      421 tatagcagcc agagttttgg cgctgtgcaa ggcgtctagg ctgagctgag gcagggtggg
      481 caattgctcc agccagtccg gcgcctggcg tttgagttct ttccagaggg ttttcgggtg
      541 gtagcgcctt tgcacccagc gctctaaaaa cgggtgggcg gtggcccaga ggtccagctc
      601 cgggtagagc tggcggccta agccttctat gttcaaaagg gttttttgca gtagcaccag
      661 ctggggctga atttccatat taaagcggcg ggcggtttgg aatagggaga tcagcgcctg
      721 gccgaaggag atgtccttca agggtttggc aaaaatcggc tcgcataccg agcgaatggc
      781 ggattcaaac gccgccaccg aggtgttctt gggcacccag ttggacacca catgcagctc
      841 ggccacctgg cggtaatccc ttctgaacat ggccagtaaa ttgcgcgcca ggtaatactg
      901 atcctcccgc gtaaggctgc ccataatggc catgtccagg gcgatgtaat ggggggagcg
      961 ggggttattg gtgctgacgt agacattgcc cgggtgcata tcggcgtgga aaaaattgtg
     1021 gtcaaacacc tgggtgaaaa atatctccac gcccaattcg gcgagggttt ttaagtccac
     1081 cccggcggct gttatgcctt tgatgtccgt cacctgaacc ccccagatgc gctccatcac
     1141 cagcacatta gtgcggcaat aatcccagta cattttgggt acatacagtt ggctaggggg
     1201 ggtgaagttg cgctttagct gggcggcgtt ggccgcttcg cgcattaaat ccagctcatc
     1261 caaaatggtg ttgcggtatt cctccactac ctctactggt ttaagccgct tgccgtcgga
     1321 gttgagcgcc gcaatccagc gggccagcac cagcatcagg cttaaatcgc tgtggatggt
     1381 ggactcaata cccggccgca cggccttgat caccacctgc tcgccactgt gtaagcgggc
     1441 gcagtgcacc tgggcgatgg acgcgctggc taaaggctcc agggaaaact cgctgaacag
     1501 aaccgcaacc ggctggccta gggcctcttc caccagggcg acaaattgct cgctggggaa
     1561 gggcgccact cgatcctgca gctggttcag gctcaagcaa atatccggcg gaattaagtc
     1621 tggccgggtg gagagcagtt ggccgaactt aacaaaaatc ggccccagat cctggcaggc
     1681 taggcgcagc cgctcaccgc ggggaagttt gagggcatcg cgcttaaaac aggcaaacag
     1741 cagtttaagc cagggctttt ggtactcctc gggcagcagc tcaagcagct ggtagcgggc
     1801 gaagacgcga agtatggtca gcaggcgttg tgaactttca aacatgcggt ttttggcgtt
     1861 cctggtccag gcgggcatca aggcgctcga ttctggcggt cagtcggtca gcgcggctgc
     1921 gcaattcgtg aatatcctgg gtgaacagat caagctgggg tttgctcggc accagttgca
     1981 gctcgttctg aacatagtcg cctaagaagg gcggcagttt tgcgagggtt tgttttagcg
     2041 tgccgcaggc ctcatacagt tgatccacca aggcccccgg cagcgggcca aggtgctcgc
     2101 tcaccacccc ggcccaatcc agctcaaggt ttttaaacag ctgtatgcag gtgttgagca
     2161 gttgaatatc cccggcgtgg ctgacgccgc tgtggctaag gctcgccgaa tccttcaggg
     2221 ctaggcccac aaagtcctgc agcttgccac tcaagcggct gttggcggcc gtctctgcgc
     2281 ccgcctccag cagcaacaga gggtagttaa ggtgcaggca ccagttaagg cgcaggtcgg
     2341 tgatctccag ttgcacggat tttccggcca agtcctccag cctgcgctgg ctcgccgggt
     2401 ccaggctcag ggcagagttg agggcggctt ctaaggcgct gcgcaaaccg gcgccgatga
     2461 gcggcaggct catgctttga agcctttgtg cagggccact atgccgccgg tgaagttgtg
     2521 tacctcgcag cgggcgaagc ctgcgtttaa taccatggtt ttgagggtgg cttgatccgg
     2581 gtgcatgcga atgctctccg ccagatagcg ataactgtcg gcatcattgg ctaccagctt
     2641 gcccattaag ggcagcaggt ggaacgagta ctgatcgtag gccttggcca gcagggggtt
     2701 gtggggtttg gagaactcca gcaccagcag cttgccgccg ggtttgagca cccgaaacat
     2761 agaggctaag gccaggtctt tatcggttac gttgcgcagg ccaaaggcga tggtaataca
     2821 gtcgaaataa ttgtcgggga agggcaggtg ctgggcgtcc gcctgtacaa aagagagatt
     2881 attcaccagg ccttgatcga tgagtttatc ccgccccaca cccagcatag aggcgttgat
     2941 atccgctaat accacctcgc cgccggggcc gactatgcgg gcaaactgag cggttaaatc
     3001 cccggtgcca cctgcgatat ccagaatttt atggcctgcg cgggcgccgg cggcctcgat
     3061 ggtcaagcgc ttccagagcc tgtgaatgcc tagggacatg acatcgttca tcacatcgta
     3121 cttagcggcc accgagtgga agaccgaggc gactttggcg gctttttcgc ctgcttttac
     3181 cgattggaat ccaaagtggg tgatttcatc acgctccata cacaggcccc tcgttcaaaa
     3241 ggcggcattc taaccctaat cggccgcagt ttctctttag gccatttcct gccgcaaaac
     3301 aaaaacggca gcaaccttgc gggttgctgc cgtttcacgg gcgtttggag ggagttaaac
     3361 caggcggcgg cgctgggcga tgaggccaag ggcgcccaag cccaacagaa tcaggctggt
     3421 gggttcgggc actgaaacac tgggggcgtc gttggtgatg gccagtaggt tatcaatact
     3481 gacattgccc tcaacgctgg tgttggcgaa gttaatcagc gagaagccgc caatacccgt
     3541 ggccgagctc acggtgaagc tctggtagag gccgggaagc tgcatagcgc cgtaggttgg
     3601 cgtcacggct tgggtggcaa ggttattacc cagggcgtca aaaaatacta tgttggtgtc
     3661 ggtgatgtaa ctggctagat ccaggctgaa gctggtgagg gaggcggcga atacatcgtt
     3721 gaataggatg gtgagatcgc tctcgtgact aatgccgtcg ttgtcgtcga tccacaggcc
     3781 gtcttggttg gagttgcggg tcgcgttgcc gaagccttta actccatagt aattcttggc
     3841 attggactgg gtgccaaacc agttggattc ccagccgggc acatcgctga agtcttcggt
     3901 taaaacgctg gcggaagcct gggaagtcag tgccagggcg gtcatcacgg caactagatt
     3961 tttcataacg ggagtctcgg ctgaggttcg gggatggttg ccaccagcaa tatgggaacc
     4021 ataaatatat tttgtatgaa aaacaatcta ttaaagacca taaaagacat gggtgacagc
     4081 ttaaatgtta agcagatcga cactgaaaat gacttttttt aggcgccgtc agaggtggga
     4141 atacattttg ccctaacccc gcaaatgacg aggtttcgtc aattagttgt cgctggggtt
     4201 tacgggctat ggccgaatcg atttttacgc atttacgagg cttgccctcc accatgacgc
     4261 gggactccgc cctggcgggg tttaggggcc tgtggcacgg caatttggcg gtgccgattc
     4321 tgcttttgat gctgctgggc atgatgattc tgcccatgcc gccgttcctg ttggacgcct
     4381 ttttcacctt caatattgcc ctctccatcg tggtgttact ggtcagtatt tacagcctgc
     4441 ggcccctaga cttcgctgtt ttcccgacca ttttgctggt ggccaccctg ctgcgcctgg
     4501 ccctcaacgt ggcctctacc cgggtggtgt tgctggaagg ccacaagggg ggcgacgccg
     4561 cgggtaaggt gattgaggcc ttcggtgagg tggtgatcgg cggcaactac gcggtgggtt
     4621 tggtggtgtt catcatcctg atgattatca atttcgtggt ggtcaccaag ggcgcaggaa
     4681 ggatttcaga ggtgagcgcg cgctttacct tggacgccat gcccggtaaa caaatggcca
     4741 tcgacgcgga tttaaacgcg ggcctgatcg accaagacga ggcccgcaag cgccgggttg
     4801 aagtggccgc cgaggcggat ttctacggct ccatggacgg tgccagcaaa tttgtgcgcg
     4861 gcgactccat cgccgggatc ttgattctgt ttattaacat catcggcggg ttaattgtcg
     4921 gcctggtgca acaccacttg gaatttcaag acgcattgca aaaatacatt ctgcttacca
     4981 tcggtgacgg cctggttgcg cagattcctt cgctactgct ggccaccgcc tccgccatta
     5041 tcgtcacccg ggtgaatacc acccaggaca tgggctccca ggtattcagc cagatgttcg
     5101 cctcccccaa agccctgggg gtttcggcgg ttattctgtt catcatgggc tccattcccg
     5161 gtatgcccca tgtggcgttt atcggcctgg gaatggtctg cgcgggcctg gcctatttca
     5221 ttcattttcg cagccaacaa gctgaggtgg tagaagaggg ccgcccccaa ggcatgccgc
     5281 gcccggcgcc tgcgcccatg ggcggcagtg gcaatgcgcc tatgaatacg ggcatagcgg
     5341 ctttagagca ccgggcttcg gcgcagtcgg aaaccgtgga ggtgtcctgg gatgatgtca
     5401 ccgccgtgga tattctcggc cttgaggtgg gttatcggct gattccactg gtggataaaa
     5461 accagcgtgg ggagctcttg gggcgaatca aaggcgtgcg caaaaagatg tcccaggact
     5521 taggctttct ggtgccgccg gtacacattc gcgacaacct ggagctgctg cccagcgcct
     5581 accgcatcac cctcatgggg gtgtccctag gggagggcga agtattcccc gaccggcata
     5641 tggccattaa ccctggccag gtgtttggcc ccctgccggg cgctaaaacc caggacccag
     5701 cctttggctt agaagccatt tggattgacg aatccctcaa ggatcaggcg caaaccctcg
     5761 gctacacagt ggtggacgcc agcaccgtgg tggctaccca cctcaaccaa attatccaaa
     5821 aacacaccca cgagctgctc ggccatgaag aagtccaaaa atggctggat ttgctggccg
     5881 aaaaatcccc caagctggtg gaggagctgg tgcccggcac catcagcctg aataccctgc
     5941 tgaaagttct gcaaaacctg ctgcgggaac aggtggccat tcgcgaccgc cgctccatcg
     6001 ccgaagcctt ggcggcctcc ggcgacaaaa gtcaagatat cgccaccctg gtgggccacg
     6061 cccgcgtggc gctggcccgt caaatcgtgc aaaacatcgt gggcagtgac ctttccatcc
     6121 cttgcataac acttgaacca tccctggaac acttgttgct tcagtcctat cagcaggcac
     6181 aaaaagctgg cgccgatggc agctccttta ttgaaccgcg cctggccgaa cgattgaaca
     6241 actccctcgt taaggccgcg caaaaacaag aagcgcaagg caagccgcaa gtactgctgg
     6301 tggccgcgcc cattcgcgcc atgctggcag gcttcgtgcg ctacaacctg ccggacatgc
     6361 aagtgctggc ttacaacgaa gtacccgaca acaaacaaat caccatagag gcgaccgtag
     6421 gtgcggaagc caagccgcag taacgcggcg gaggaatgag tcatgactgt aaagcgtttc
     6481 gtagcacccg atatgcgcag ggccctggat ttggttcgcc aagaaatggg gccggaggcc
     6541 atcatcctct ccagtaagcg cataaaagag ggtgtggaaa ttctgacggc ggatcagctg
     6601 gacattccca cccgcggcta cgacgctcgc aaacagttcg gcgagcaatt cgactccgac
     6661 ctcgaccgcc ccctgatctc cgaccgggac tggcagccgg atacccgtct gcccgccaaa
     6721 ccccaagcgc ccctggctcc ccccgtcaac tcgcaagctg agcgggccgt ggccctggcc
     6781 aaagaaattg aagctgcccg cgcccgtatg ctggcggcga aaaaatccgc ccaggcggcc
     6841 aatcgcccca tcggtgaatt gctggccggt cagcgcgccg agccaagcct gccgcccgcg
     6901 gccgccagcg aaaccgtcgc gcggtttgat aagcccaacc gtattgagcc gcgggtatca
     6961 ggcctcagcc cggcagagac cgccgacccc ttcgaatttg attttggctt tgatcagctg
     7021 gtggcggtta agcctgcccc cctgcaagac gaggccaggc ccgatgggca gttggcgaat
     7081 ttggccgccc aggtgggcca gctgcaggag cgagccccgg tggcccgcag cgacgacacc
     7141 cggctcaacg cactacaggc ggaaatcgcc gatatgcgca gcctgctaga gcgccatttg
     7201 cgccactggc ccgcctccac cgaagaattc accagtaagc ccagtgccgg gcactacgcc
     7261 ctcggccagc agttacagcg tttgggcata ggtgcggagt tggtggagcg cctgtgccag
     7321 cactgcgcca atcagccccg gttaaatctc gcctggcggg aagccctcaa cgaattggta
     7381 gctagattgc cggtgcagag tacggacccc atcgaccaag gtggcgtgtt tgcctttgtc
     7441 ggccctaccg gcgtgggcaa aaccaccacc atcgccaaat tggccgcccg ctatgtactg
     7501 gctcacggcc agggcaaggt ggcccttgtc accaccgata cctaccgggt gggcgcgcaa
     7561 gatcaactgc gctccctagg ccgcatcctc aatgtaccgg tgcgcattat cgaccaagag
     7621 cgagcattgc ccagcgtgct ggcgagcctt aaagcctatc cactgattct cattgataca
     7681 gccgggttcc ggcagggcga cccactgctg cgggagcaag aggctatttt ggccgcctgc
     7741 ccaagcgtgc agcgggtatt ggtgatggcg ggcaacagcc aagtgcaaac cctcaaagcc
     7801 agcgcccacg cctataaggc ccgccgctta gctggttgcg tgttgaccaa aattgatgag
     7861 accgcaagcc tcggcgaggc catcagcgtg gtcatccaac aggccattcc tctgttgtac
     7921 accaccttcg gccaagagat ccccaaagac atagccgtgg cctccgccca gtctttagtg
     7981 gccaaggcgg tgaatctggc caaacaggaa agcgcacagg agagcagcgt ggggcttagc
     8041 tagggcgggt gcggaattta gagcgcccaa gagcaaagca tctatactca aaaacagcaa
     8101 cacccggcgt ggtctgcccg gtgagccaac agaaaggttg taagttgcat gatggcaagt
     8161 cgtcccgtaa aagttgtcgc gatcaccggt ggtaagggtg gcgtgggcaa aactaatctc
     8221 tccgtcaacc taagcatcgc cctggccgag atgcgccgcc gggtagtact gctggatgcg
     8281 gaccttgggc ttgccaatgt ggatgtatta ttggggttgc aggcgcgttt taccctggcc
     8341 gatgtgctca gcggcgagcg agacttgcgc gaggtgatgc tcaacggccc ggccggtttg
     8401 aaaattgtgc cggcctcctc gggcctgcaa aacatggtga agctctccgc ccaggagcac
     8461 gccgccatag tgcacgcctt cagtgaactc tctgatcaaa tcgacgtgct ggtcatcgac
     8521 accgccgcag gtatttccga caccgtcacc agctttgtac gcgccgccca ggaagtggta
     8581 atcgtggtgt gcgatgagcc ttccagtatc accgacgcct atgcgctgat gaaattactc
     8641 aacaccgaac acggcattac ccgctttaaa gttatagcta atatgacccg caccctgcag
     8701 gagggcagcg ccctgtttaa taaactcaac gccgtctgcg agcgcttctt agacgcttct
     8761 ttacagtatt tagggccagt gccctttgat gaaaacgtgc gtaaagccgt gcaaaaacgc
     8821 aaagccctgc tggaattcgc cccccgctgt aaggcctcgc aggcgattcg ttttattgcc
     8881 cagcgcatcg atcaatggcc ggtgcagaac agcgctcgcg gtcatctgga attctttttt
     8941 gagcggctcg ttgcctccgc cagtggtggc atgtgacagc agcgcggctt tccatgcgtt
     9001 ttgcagcggt taatcaggag ccaggagtgg acatcgaagc ctttgccccg ctggtaaaaa
     9061 aaatcgccta tcacttactg gcgagaatgc ctgccagcgt gcaattggac gatctgattc
     9121 aggcgggcat gttgggccta atagaagctg cacaaaaata tgacgacagc aaaggcgcaa
     9181 gctttgagac ctacgcgggc attcgcatca aaggcgccat ggtggatgaa atgcgccggg
     9241 gcgactgggt gcctcgctca gtacaccgca acgcccgcaa aatcagccaa gccttggcga
     9301 gcctggaggc aagcctaggg cgtgaggcct gtgatgccga agtggctgct catcttgaaa
     9361 tgtccatcga agagtaccag gcccttttag ccgacaccca aggcagccgc ctatttagct
     9421 acaacgaaga catagacgat gaagaatcct gcgcatccgc cgatgattcc agtgctccgc
     9481 tgcaggatcc ggccaagtcc gtagcgcaga cccatttgca atcctccctg gccgccgcca
     9541 tagctgaatt gcccgagcga gagcagctgg tgttatccct ctactacgaa caagaaatga
     9601 accttaagga gatcggcctg gttctggggg tgtccgaatc ccgcgtcagc caaatccact
     9661 cccaggccgc cctgcgcctg aaaggcaaac tctccggctg ggcgcgctaa tccccaaacc
     9721 cccagcccat tttagggatt tgccgctaat taccctaaaa actgcagtca acctcgctac
     9781 gagttcaact gcggttttta ggattatcag cgctttcaat gctacacgcc gtcctctgcc
     9841 cgcctcatcc cgtaagattt tccttaagct cgcgccgcac ctgtttcccc cgatgagaaa
     9901 attggtaaca aagtatgagc gttaacctct aatggttaat aagacctcac cgccttgtaa
     9961 ggcccgttaa tgctttagtt gggacaatta ccctgctaac taatttattt gctgcaattg
    10021 caaaatgttt cttttctacc cgtttttctg cgcctatact ttgctcaatt cggtcttgga
    10081 ggtgcttatg aaacgcttag gtttttgttc ttgggtagtc ttagtagggc tgataagtgg
    10141 caatgcgatg gccgcgcagt cgttgagtga agccaaaaaa atcgttgctg attatcaaat
    10201 attgcgcgag gcctgctccg atgccaccgg cgcaaaacgc cttgagtgca tggcccgctt
    10261 gtcctcggcc agtgatagct accgggaggc taaaaccttc gtcatggcaa gttctggtag
    10321 tacccaactg aaaattgcca aagcccatta aggcttctat caagcctagg tgtatccagc
    10381 ccgagggatc acctgcctag acccacacct tcttatacgc tcctcgccaa tacgatgccg
    10441 tggtattggc tccgggcatc tcttcttcct tcttggaaac taaatctatc gtgcctgtgc
    10501 ctaaattcag gctgtacctt gggcgctatg aggtgggtta tgcgccggtc aatttttagg
    10561 tttctctgta tcctgtattt acccttttgc atgcaccgct ctactcgccc ctagtcttta
    10621 aatcctgtat tctgcaggcc cttacgagcc ctgcctgtgc tgggcgtgac gcattttttc
    10681 aaataggtat ttctcaccgt gtacgtttac gacgaacatg acagcaaaat tattcacgag
    10741 cgggtggctc agttccgctt tcaaacccag cgtttcctag acggcaaatt aaagcctgaa
    10801 gagtttttgc cgcttcggct gcaaaacggc ctctacgtgc aacgtcttgc tcctatgctg
    10861 cgcatagctg tgccctacgg catgttgaac gccgtgcaac tgcgtaagct cgcttatatt
    10921 tgccgacact acgataaggg ctactgccat gccaccacac ggcagaatat tcagctcaac
    10981 tggccgcaac tagaggaggt gcccgacatc ctggccgagt tggcccaagt g
//
LOCUS       DAOQGS010000059        11030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2759_length_11030_cov_4.713166, whole genome shotgun sequence.
ACCESSION   DAOQGS010000059 DAOQGS010000000
VERSION     DAOQGS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_11030_cov_4.713166"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PKZ52_03615"
     CDS             <1..1026
                     /locus_tag="PKZ52_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG58888.1"
                     /translation="PLQLKAFSLEALIKDIANHYAVQGTQECVLKFSQNPSIATWVIA
                     DEGKIKQIIYNLINNALKHSQCNTIECSLDCVSVHNNQGELQLDIRDNGKGVERAKIS
                     QLTRRFEQNEYSQKGGVGLGLSIVIEMLAAMDGSLEIDSDTGKGFSTRCTIPIECCAA
                     QASEPTVATAPPANAIAATDTHLIMPADSSHSRTPVEPAATPETPATINDYFVLIVED
                     NPVNQMVIEGMLKKLELPFKTAANGAQTRALYRQFKGKFACILMDIQLPDASGLDLIR
                     EIRAEDDPVKILVISAFAFNEDEETAKRAGADGYLRKPYHFDHFKNALTVLYQTQAPT
                     KPTAANL"
     gene            complement(1205..2926)
                     /locus_tag="PKZ52_03620"
     CDS             complement(1205..2926)
                     /locus_tag="PKZ52_03620"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNG58889.1"
                     /translation="MRTSQYLIATLKETPADASVISHQLMLRAGMIRKLASGLYSWLP
                     LGLRVLRKVEAIVRTEMNNAGAQEVLMPVVQPAELWQESGRWEQFGPELTRFKDRHSN
                     GFCLGPTHEEVITDLIRSEINSYKQLPANFYQIQTKFRDEVRPRFGVMRAREFIMKDA
                     YSFHPSHDCLLKTYEVMHQTYCAIFQRIGLNFRPVLADTGSIGGTGSHEFHVLADSGE
                     DDIVFSDASDYAANIEMAEALAPSVQNEPKRSAQTVATPGCKTIDEVCSFLQVSADKT
                     LKTLIVLGAADEAGKQPLIALVLRGDHQLNEVKASKLKQLAVPLTFASDQRIADELGL
                     PVGYLGPLQLGIPVIADRAAAAAINFVCGANTAEHHVVDANWGENAAFTEVADIRNVV
                     KGDPSPCGKGQLDIKRGIEVGHIFQLGTKYSEAMKARVLDENGKEQTMVMGCYGIGVS
                     RVVAAAIEQNHDEKGIIWPHAIAPFHVALIPINLHKSPAVAEVCEALYQQLTAAGIEV
                     LFMDEAKARLGAMLADAELMGLPHRLVVGDRGLEQGVVEYKGRTAVDSENIAIADVLA
                     TIKNRML"
     gene            3249..3683
                     /locus_tag="PKZ52_03625"
     CDS             3249..3683
                     /locus_tag="PKZ52_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNG58890.1"
                     /translation="MFELHPQLKADSYLIGKFSLSWVLLHKDATYPWVILVPAREDLR
                     EIHHLDSDDQISLMRESCHLSEVMVDLFAPKKLNVAAIGNIVSQLHLHHVARFEQDPA
                     WPKPIWGLLPATGYSEELRLQRINQLRSALVGEGFIAQDHWS"
     gene            complement(3963..4382)
                     /locus_tag="PKZ52_03630"
     CDS             complement(3963..4382)
                     /locus_tag="PKZ52_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNG58891.1"
                     /translation="MAAPKAKKPAPKKAPAKEAVKKVAKKVEAKPAKALAGVKERFSK
                     AELLTHIAEATSLSKKDVAGVIDTLANVIEAHIGKKGCGEFVLPGILKVATVKKAAKK
                     ARKGINPFTGEETTFKAKPASIAVKIRPLKKLKAMVE"
     gene            4987..5280
                     /locus_tag="PKZ52_03635"
     CDS             4987..5280
                     /locus_tag="PKZ52_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNG58892.1"
                     /translation="MPFYEYQCTACHHELEALQKLSDAPLSLCPACNANTLVKKVSAP
                     GFRLAGGGWYETDYKSGNKKNLVDKGESGTSASAEKPPAAAKEPAPAASSSKE"
     gene            5304..7076
                     /gene="aspS"
                     /locus_tag="PKZ52_03640"
     CDS             5304..7076
                     /gene="aspS"
                     /locus_tag="PKZ52_03640"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNG58893.1"
                     /translation="MRSVYCGALRAADIDKEVQLCGWVDTRRDHGGVIFIDLRDRDGI
                     VQVVFDPDAKSSFELANRVRSEYVLQVRGKVRARSPNTVNKNMGTGEIEVYGLELAIL
                     NTAETPPFPLDASNVGEDVRLRYRYIDLRRNQMQNNLRFRSKVTNLMRNYLDANGFLD
                     IETPILTRATPEGARDYLVPSRTHEGKFFALPQSPQLFKQLLMVAGYDRYYQIAKCFR
                     DEDLRADRQPEFTQIDIETSFMNEEQIMAVTEGMIRDLFANLIQVDLGVFPRMPFSEA
                     MEKYGSDKPDLRIPLQLVEIKDLMDEVEFKVFAGPAKDPKGRVTALNVPGAGEKLSRK
                     QIDDYTKFVSIYGAKGLAYIKVNDINDLEEGLQSPIVKHLPPQVRREILNRVNAQNGD
                     IIFFGADNGKIVSEALGALRCKLGADLDLYTCEWAPLWVVDFPMFEVTEQGLSACHHP
                     FTSPSCSIEELKANPAPALSRAYDMVLNGTELGGGSIRIHDQNLQQAVFEILGITPEQ
                     QREKFGFLLDALKFGAPPHGGLAFGLDRLIMLMTKSDSIRDVIAFPKTQSAACLMTDA
                     PGVVDTLQLRELHIKVNTKETKNT"
     gene            complement(7209..7559)
                     /locus_tag="PKZ52_03645"
     CDS             complement(7209..7559)
                     /locus_tag="PKZ52_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNG58894.1"
                     /translation="MSEKTIFSRIIDGEIPSQFIYQDELCVCIKDIAPQAPVHLLISP
                     RKPIPRLADATAEDKAILGHLMWAAGEIARQQGVAEAFRLIVNNGAGAGQTVFHLHLH
                     LMAQRQFHEGQMWE"
     gene            complement(7581..7787)
                     /locus_tag="PKZ52_03650"
     CDS             complement(7581..7787)
                     /locus_tag="PKZ52_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNG58895.1"
                     /translation="MIDKKLLSILVCPVSKAPLTWVADSNELVCLASSLAYPVRDGIP
                     VMLESEARQISLEEKEALTAKANI"
     gene            complement(7831..8694)
                     /locus_tag="PKZ52_03655"
     CDS             complement(7831..8694)
                     /locus_tag="PKZ52_03655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNG58896.1"
                     /translation="MVNYLKLYWLFLIVALSFSAQASAAEITTQTKIGPVKIAGLWQQ
                     GELLHGQAPSGAQVEFLGQKPVILPDGRFLLGLDRDAPARVSIRVIHKGQAWEQAQAV
                     VPRQYNLQRVNGVEDKYVNPDPAQTARIAREAKLIAQARARRDAFEAAWQPFNWPAKG
                     PISGVYGSQRIFNGVPKRPHYGIDIAGPVGAKVFAPQAGLVTLAEADLFYSGGTIILD
                     HGLGLTSTCMHLSKVLVKSGERVKQGDLIGLIGKTGRVTGPHLDWRMNWHNQQIDPEP
                     LVRGKPMPEVN"
     gene            complement(8911..10824)
                     /locus_tag="PKZ52_03660"
     CDS             complement(8911..10824)
                     /locus_tag="PKZ52_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG58897.1"
                     /translation="MPLLKLDNLSLAYGQQVLFDSIQLLLNKGDRLCLVGRNGVGKST
                     LLKVIEGLVESDGGSRWLDTAVKVAGLSQDLPEANDERVLDYVASGLAQLGQDLAQYQ
                     RCIEAPDADLNLLAQLQSRIEAADGWSLQNRIEQVLSRLELSANDRLGDLSGGWRRRV
                     ALARALVCQPDVLLLDEPTNHLDIKAIEWLEAEIKQFSGAVVFITHDRAFLRAVANRI
                     GELDRGKLAIWDGNYTDFLAFRERQLAEEEKHNAEFDKRLAKEEIWIRQGIKARRTRN
                     EGRVRALKAMRQERAERRSQLGTVKMALQNEGNSGKLVAEFSDVCFSYGDKLLIDRYT
                     GVITRGDKVGLIGPNGVGKSTFLKLITEELKPTSGEVKLGTNLAVAYFDQLRDQLDLD
                     KNAIDNVAEGRDFIEINGQNRHCISYLSDFMFTGERARTPIRALSGGERNRILLAKLF
                     SKPANVLIMDEPTNDLDAETLELLEELLVDYKGTLLLVSHDREFLDNVVTSTLGFEGN
                     GKLADYVGGYQDWQRQGGKWADTSRAAKPQKSLEKSAAVAPPAKTAESPAKKPASKLS
                     YKLQRELDALPGQIEQWEAELLELSNQMAQPEFYNRSPEAIAAASQVIADKEAALQVA
                     YQRWDELENLKGE"
BASE COUNT         2711 a         2902 c         2656 g         2761 t
ORIGIN      
        1 cctttgcaat taaaagcatt tagcttagag gcgctgataa aggatatagc caaccattat
       61 gcagtccagg gtacacaaga atgtgttttg aaattcagcc aaaatccctc tatagccact
      121 tgggtaattg ctgatgaagg caaaattaag cagattattt acaacctaat taacaacgct
      181 cttaaacaca gtcagtgcaa cacgattgag tgttccctcg attgtgtctc tgtacacaac
      241 aaccagggcg agttgcaact cgacatccgc gataacggca agggagtgga gagagccaaa
      301 attagccagc taacccgtcg ctttgagcag aatgaatatt cgcagaaagg cggcgtgggc
      361 ttaggcttga gcatagtgat tgaaatgtta gccgcaatgg atggcagctt agaaattgac
      421 agtgatacag gtaaaggatt tagcacccgc tgcaccatcc ctattgaatg ctgtgcggca
      481 caggcaagcg aaccaaccgt tgcaaccgcg ccaccggcaa acgcgattgc ggccacagat
      541 acccatctca tcatgcccgc cgattcgtcc cactcccgca caccggtgga gcctgccgca
      601 acaccagaaa ccccagcgac tattaatgat tattttgttt tgatcgtgga agataatccg
      661 gtcaatcaaa tggttattga aggcatgtta aaaaaactgg aactgccctt taaaacggcg
      721 gcaaatggcg cccaaacgcg cgcgctctac cgtcagttta aaggaaaatt tgcctgtatt
      781 ttaatggata tacagctgcc cgatgccagc ggcctggatt taattaggga aattcgcgcg
      841 gaggacgacc cggtgaaaat actggtcatc agcgcctttg catttaatga agatgaagaa
      901 acggccaaaa gggctggcgc agacggttat ctgcgcaaac cctaccactt cgaccatttt
      961 aaaaatgcct tgacggtttt ataccaaacc caggccccca ccaagccaac tgccgccaat
     1021 ctttaagccg gttataaata tttcgcacaa tttcacgcgg gacgaatttg gggggaatta
     1081 gtcaagcggc attctcgatt ctgccgctta gctaaatctg tcttaatttg ccacccgcgc
     1141 ttacgagggt aataagctgt gcttaatagt tacgagcagg gtggtatttc aacaagctgt
     1201 taaactagag catacgattt ttaatggttg ccagcacatc ggcgatagcg atgttttcac
     1261 tgtcaactgc cgtgcgccct ttatactcaa ccacgccctg ctcaaggccg cgatcaccca
     1321 ccaccaggcg atggggcaag cccatcaact ccgcatcagc gagcatagct cccaaacgag
     1381 ctttggcttc atccataaat aaaacctcga tgcctgccgc ggttaattgt tggtacaagg
     1441 cttcacagac ttcggcgacc gcaggggatt tatgtaaatt aataggtatc aacgccacat
     1501 gaaatggcgc tatggcatgg ggccaaataa tgcctttttc atcgtgattt tgctcaatgg
     1561 cggcagccac cacacgggaa acgccgatgc cataacaacc catcaccata gtttgttcct
     1621 tgccgttttc atccaatacc cgggctttca tggcttcaga atatttggtg cccaattgga
     1681 aaatatggcc cacctcaatg ccgcgtttaa tatcgagttg gccttttcca caggggctgg
     1741 ggtcgccttt tactacgttg cgaatatcgg caacttctgt aaaggcggca ttctcacccc
     1801 agttcgcatc cactacatga tgctcggcag tatttgcgcc acagacaaaa ttaattgccg
     1861 cagctgctgc acgatcggca atcaccggga tacccagctg caatggcccc aaatagccca
     1921 ctggcaaacc caattcatcg gcaatccgct gatcgctggc aaaggttaag ggcaccgcca
     1981 gttgtttaag tttgctggct tttacttcgt ttaattggtg atcaccacgc aataccaacg
     2041 cgattaacgg ctgtttgccc gcttcatcag cagcacccaa cacaatcaac gttttcagcg
     2101 ttttatcggc agacacctgt aaaaagctgc acacctcgtc aattgttttg cagccgggtg
     2161 tggccactgt ttgtgcagat cgctttggct cattttgaac actgggagca agcgcctcag
     2221 ccatttcaat gttggcggca taatcgctgg cgtcactaaa aacaatgtca tcctcaccgc
     2281 tgtccgctaa aacgtgaaac tcgtgggaac ctgtaccccc gatggagcct gtatcggcta
     2341 atacagggcg aaaattaagg ccgatccgct gaaaaatagc gcaataggtt tgatgcatta
     2401 cctcataggt ttttaacagg caatcgtggg aggggtgaaa cgaataggcg tccttcataa
     2461 taaattcccg cgcccgcatt acgccgaagc gggggcgcac ttcatcgcga aattttgttt
     2521 gaatttgata aaaattagcg ggcaattgtt tgtagctatt aatctcggag cgaattaaat
     2581 ccgtgatcac ctcttcgtgg gtgggcccca aacaaaaacc attgctgtga cgatctttaa
     2641 agcgcgtaag ttcaggacca aattgctccc agcggccaga ctcttgccac aattccgccg
     2701 gttgcacaac gggcatcaaa acttcctggg caccagcatt gttcatttca gtgcgcacaa
     2761 ttgcctctac tttgcgcagc acacgcagcc ctaagggcaa ccaagaataa aggcccgatg
     2821 ctaatttgcg aatcatgccc gcgcgcagca tcagttggtg gctaataacg ctggcatcag
     2881 caggggtttc ttttagtgtg gcgattaagt attgactggt gcgcatgggt ttctcattat
     2941 gactaaaata ggctttttta aaggcggttg atattcgcag ccgcttttga tgggttttct
     3001 gcggtaatgc gtggcgtaca gggctgtact gctaaatgtg atttaaccac atgaataagg
     3061 ctaaccactc actccacggg catggtacct agtccgcagg tctcacctcc aatgcacttg
     3121 gcctacttta taggtagaac taaagcaccc tatcactgat agtgcaaacc tgcgatattt
     3181 caacgggtac attagcggtt ggctttgcag ctattgtatt tgacacagca cataggagta
     3241 cagaacccat gtttgaatta caccctcagt taaaagcgga cagctatttg atcggtaaat
     3301 tttcgctctc ctgggtgctt ctccataaag atgccaccta tccctgggtc atattggtgc
     3361 ccgcacggga ggatttgcgg gaaattcacc atttagatag tgatgatcaa atatccctga
     3421 tgcgcgaatc ctgccattta agcgaggtga tggtggattt attcgcgcct aaaaagttaa
     3481 acgtcgccgc cattggcaat attgtgagcc aattgcattt acaccatgtt gcgcgctttg
     3541 aacaggaccc cgcctggcct aagcccatct gggggctttt gcccgccaca ggttatagcg
     3601 aagaactgcg cctgcagcgc atcaatcaac tgcgcagcgc tttggtgggt gaggggttta
     3661 tagctcaaga tcactggagt taggcaatgt gtatggctgc cagtgttaaa cctaaaaacc
     3721 gcagttaaat cctactgcgg tttttaggtt aaaacatagg ctgtttacgc tgaataaaca
     3781 ggcaaataca cagcaatgcg cctacacggg agcagacagc aggaggaaat gagcccagat
     3841 tagcaggcaa aaaaaatgct gcgtaaggca gcattttttt agtctggctt ttaggaagcc
     3901 actaaagttg aaaccgctcg gcgcttttga gcctgtagac agctaaaaaa cgctcgatca
     3961 gcttattcaa ccatagcttt gagttttttc agtggacgga ttttaaccgc aatactggct
     4021 ggcttggctt tgaatgtggt ctcttcgcca gtgaacggat tgatgccctt acgcgctttt
     4081 ttagcggctt ttttcacagt ggccactttt aaaatgcctg gcagtacaaa ctcgccacag
     4141 ccttttttgc caatgtgcgc ttcgattaca ttggccagtg tatcaataac acctgccaca
     4201 tcttttttgc tcaggctagt cgcttcagca atgtgtgtga gcaactcagc tttagaaaag
     4261 cgctctttaa caccggcgag cgccttggcg ggtttagctt cgactttttt agcaactttt
     4321 tttacggctt ctttagctgg agcttttttg ggagcgggtt ttttagcttt gggggctgcc
     4381 atggtatgaa cctcaatggt ttttggaatg gaaatacgac gttattttat gcaatattta
     4441 tagagtattg tattcattca agcaaccccg cgcgtgcaaa aagcatagaa aaacgcacat
     4501 attcaaccgc aataagcaaa ataagccacc cgcactgctc tttttagggc atttttctgc
     4561 tctgttgcac cgcctgtaac acccataacg gataaaaagt cttcaatcaa agcgcctctt
     4621 atcctgctta cgcaaaatat tgcgtggcaa ttcgcagtgc cactgacaat aaccaaaggg
     4681 tgtaaatctg ctgtaacgca tcttttccac cgctcgatgg gatatacgca aacacctaag
     4741 gggcacaagt gcacagcccc aagcctgctg ccgcgatgat tatgcaaaca ccggtaaacc
     4801 cctgtttcca aaggcttcca aagatgccgc attcaggggc gtggactcag gtgcaacaag
     4861 ccctattttt agtcgccatt agcggtgttt tgtgggtggg cggctcatgt ataatgcgcg
     4921 ccccgcgccc cagcatgggc ctgcgcggcc gtgatttttg tttccccatc aagaccgagg
     4981 tttcgcatgc cgttttatga gtaccaatgt accgcctgcc accacgagct agaagcgctg
     5041 caaaaattaa gtgatgcacc gctcagcttg tgcccagcgt gcaacgccaa cactctagta
     5101 aaaaaagtat cagcgccggg atttcgttta gccggcggcg gctggtatga aacagactac
     5161 aaatcaggca acaaaaagaa tttagtggac aagggcgaaa gcggtacgtc cgccagtgct
     5221 gagaaaccgc cagccgccgc gaaagagcca gcgcctgcgg cctcgtccag caaagaataa
     5281 actccatcga ataggtacaa accatgcgca gcgtttattg tggcgccctc cgggccgccg
     5341 atatagataa agaagtccag ttatgcggtt gggtggacac ccgccgcgat cacggtggtg
     5401 ttatttttat cgatctgcgc gaccgcgatg gtattgtgca ggtggtattt gacccggacg
     5461 cgaaaagcag ttttgaactc gccaatcgcg ttcgctctga atatgtatta caggtgcgcg
     5521 gtaaagtgcg cgcccgctcc cccaacaccg tcaataaaaa tatgggcact ggggaaattg
     5581 aagtctatgg ccttgagttg gccattctca acacagcgga aactccaccc ttcccccttg
     5641 atgccagcaa tgtaggtgaa gatgtgcgcc tgcgttatcg ctacattgat ttgcgccgca
     5701 atcagatgca aaacaacctg cgctttcgct ccaaagtcac caatttaatg cgcaattatc
     5761 tcgacgcaaa tggctttttg gatatcgaaa cccctatcct cacccgcgca acgcccgagg
     5821 gcgcgcggga ttatttagtg ccctcgcgca cccacgaggg aaaatttttt gctctgcctc
     5881 aatcgccgca attgtttaaa caattgttaa tggtggccgg atacgatcgc tattaccaaa
     5941 tcgccaaatg tttccgcgat gaagatttgc gggctgaccg ccaaccggaa tttactcaga
     6001 tcgacatcga aacctcgttt atgaacgagg agcagatcat ggccgtcacc gaaggtatga
     6061 tccgcgattt attcgccaac ctgattcagg tggatttagg tgtattcccc cgcatgccat
     6121 tttctgaagc catggaaaaa tacggctcag acaaaccgga cctgcgcatt cccctgcagc
     6181 tagtggaaat taaagacctg atggatgagg ttgaatttaa agttttcgca ggccctgcca
     6241 aagatcccaa aggccgcgtt accgctttaa atgttccagg tgccggcgag aaactctccc
     6301 gcaaacaaat cgacgattac accaaatttg tcagcattta tggcgccaag ggccttgcct
     6361 atatcaaggt gaacgacatt aacgatctgg aagaaggcct gcagtcgccg attgttaaac
     6421 acttaccgcc ccaagtgcgc cgggaaattt taaaccgcgt caatgcccaa aacggcgata
     6481 ttattttctt cggcgccgac aacggcaaaa ttgtctctga agccctcggc gcattgcgct
     6541 gtaaattagg tgcggattta gacctgtata cctgcgaatg ggcgccgctg tgggttgtgg
     6601 atttccccat gttcgaagta accgagcagg gtttaagcgc ctgccaccac ccgtttacct
     6661 caccctcctg cagcattgaa gaacttaaag ccaaccccgc ccctgctctg tctcgcgcct
     6721 acgatatggt attaaacggt acggaacttg gcggcggctc cattcgtatt cacgatcaaa
     6781 acctgcagca ggcggtgttt gaaattctcg gcattacgcc cgaacagcag cgggagaaat
     6841 ttggcttctt gctggatgcg ttaaaattcg gcgcgccacc ccacggcggt ttggcgtttg
     6901 gtttggatcg actgattatg ttgatgacca aatccgactc cattcgcgat gttatcgcct
     6961 tcccgaaaac ccaatccgct gcctgtttaa tgaccgatgc accgggggtg gtggatacac
     7021 tgcaattgcg cgagctgcat attaaggtaa ataccaaaga aaccaagaac acttaaacca
     7081 ctcataaaaa atccccgcca cctgtaaagg ccgcggggat tttttttgcc ctaagagttt
     7141 aattcagcct agattgcaag catcctacac aactgcaatt gcagccggaa aagcttaatt
     7201 aacctgaatt attcccacat ctggccttca tgaaattgtc tctgggccat taaatgcagg
     7261 tgcagatgaa acaccgtctg ccccgcccca gcgccattgt tgacaattag acgaaaggct
     7321 tcagccaccc cctgctggcg ggcaatttct ccggccgccc acattaaatg ccccaggatc
     7381 gctttatctt ccgccgtagc gtccgccagg cgcgggatgg gtttgcgggg gctaattaat
     7441 aagtgcacag gcgcttgggg tgcaatatct tttatacaca cgcatagctc atcttggtaa
     7501 ataaattggc tgggaatctc gccgtcgata atgcggctga aaatagtttt ctcactcatg
     7561 gcgtgcccct gtgttaggcg ttaaatatta gcttttgctg tgagggcttc tttttcctca
     7621 aggcttattt ggcgcgcttc agattccagc atcaccggaa tgccatcgcg caccggatag
     7681 gcaagggagc ttgccaaaca caccagctcg ttggaatcgg ccacccaggt taggggcgct
     7741 ttgctcacgg ggcagaccaa aatactcagt aattttttat cgatcataca atcacactct
     7801 tgttgccgct tttaacattt tgccgggtaa ttaattgacc tcaggcatag gtttaccccg
     7861 caccaaaggc tcagggtcta tctgctggtt gtgccagttc atgcgccagt ctaaatgggg
     7921 gccggtgacg cggccggttt tgccgatcag gccaattaaa tcgccctgct tcacccgctc
     7981 accgctttta actaacacct tgcttaaatg catgcaggtg gaagtcagcc ccaggccgtg
     8041 atctaaaata atagtgccgc cggaataaaa caaatccgct tcggccaggg ttactaaacc
     8101 ggcctgcggc gcaaatactt ttgcccccac cggccctgct atatcaattc cataatgggg
     8161 ccgcttgggc acaccattaa aaatgcgttg gctaccgtat acgccgctga tggggccttt
     8221 ggcgggccaa ttaaaaggtt gccacgcagc ttcaaatgca tcccggcggg cgcgggcctg
     8281 ggcaattaat ttagcttcgc gggcgatgcg cgcggtttgc gctgggtctg gattaacgta
     8341 tttatcttcc acgccgttca ctcgctgcag attatactgg cggggcacaa cggcttgtgc
     8401 ttgctcccaa gcctggcctt tatgtatcac ccgaatactc acccttgcag gtgcatcgcg
     8461 atcaagccca agcaaaaaac gcccgtcggg caggataacc ggcttttggc ctaaaaattc
     8521 cacttgggcg ccgcttggcg cttggccatg taacaattcc ccctgctgcc aaagcccagc
     8581 aattttaacc gggcctattt ttgtctgtgt tgtgatttct gccgccgaag cctgggcaga
     8641 aaaacttagt gccacgatca gaaaaagcca atacagtttt aaataattaa ccatggttta
     8701 acaccctgac tgaagacagt cattcgtatc tggcgggcca aatgcccaat tatcagccgg
     8761 agaaaatgca gtttttgatc ggctcatagg catgcattca cggcctgtct gcaatgactt
     8821 agactgattg cctgcatcta acaggcggat caaattgaac gcctttttca gttctattaa
     8881 cccggcactc aatactgccg ggttaataga ttattcgccc ttaagatttt ctaattcatc
     8941 ccaacgctga taagccacct gcaaagctgc ttctttatcg gcaatcactt ggcttgccgc
     9001 tgcaatagct tcaggggagc ggttgtaaaa ttccggctgg gccatttggt tggataattc
     9061 caacagttcc gcttcccatt gctcaatttg cccgggcagt gcatccaatt cacgctgcaa
     9121 tttataactt aatttactcg ctggcttttt cgcaggtgat tcagccgttt tcgccggggg
     9181 cgcaactgca gcggattttt ccaggctttt ttgcggcttg gcggcgcggc tggtatctgc
     9241 ccatttgccg ccctggcgct gccaatcctg ataaccgccc acgtagtcgg caagcttgcc
     9301 attgccttca aagcccaggg tggaggtgac gacattatct aaaaattccc ggtcgtggct
     9361 caccagtaat aaagtgcctt tatagtccac caataattct tccaataatt ccagggtttc
     9421 cgcatccagg tcgttggtgg gttcgtccat gattaacaca ttggcgggtt tgctgaataa
     9481 ttttgccaat aaaatacggt tgcgctcacc gcctgacagg gcgcgaatgg gcgtgcgggc
     9541 gcgctcgccg gtgaacataa aatcgcttaa ataactgatg cagtggcgat tctggccgtt
     9601 gatttcgata aagtcgcggc cctccgccac gttgtcgatg gcgtttttat ccaggtctaa
     9661 ctgatcgcgc agctggtcaa aataggccac ggcgaggtta gtaccgagtt ttacctcacc
     9721 gctggtgggt ttaagctctt ctgtgatgag ttttaaaaag gtacttttac cgactccgtt
     9781 ggggccgatt aaacccacct tatcgccacg ggtgataaca ccggtatagc ggtcaattaa
     9841 taatttatcg ccgtaggaaa aacacacatc actaaattcc gccaccaatt tgccggagtt
     9901 gccttcattt tgcagagcca ttttaactgt gcccaattgg ctgcggcgct cggcgcgctc
     9961 ttggcgcatg gcttttaggg cgcgcacgcg gccttcattg cgggtgcggc gggctttaat
    10021 gccctggcga atccaaattt cttccttcgc caggcgctta tcaaattcgg cgttgtgttt
    10081 ttcttcctcc gccaattggc gttcgcgaaa ggcgagaaaa tccgtgtaat tgccgtccca
    10141 aatggcgagc ttgccgcggt ctaattcgcc gatgcgattg gccaccgccc gcaaaaatgc
    10201 ccggtcgtgg gtgataaata ccacggcgcc ggagaattgt ttgatctccg cttccagcca
    10261 ctcaatggct ttaatatcca gatggttggt gggctcgtcc agcaggagca cgtccggctg
    10321 gcacactaag gcccgcgcca gggccacccg gcggcgccaa ccgccggaga gatcccccaa
    10381 gcggtcgttg gcggagagct ctaaccggct gagcacctgt tcgatgcggt tttgtaaact
    10441 ccaaccatcc gccgcttcga tgcggctttg caattgggct aagaggttta aatccgcgtc
    10501 cggcgcttcg atgcagcgct ggtattgggc gaggtcctgc cctagttgag ccaagccaga
    10561 ggccacatag tccagcaccc gctcatcgtt ggcctccggt aaatcctggc ttaagcccgc
    10621 aactttaact gccgtatcca accagcggct gccgccatcg ctctccacca agccttcgat
    10681 gactttaagc agagtggatt tgcccacccc attgcggccc accaaacaca ggcggtcacc
    10741 cttgtttaac agcagttgga tagaatcgaa taacacctgc tggccatagg caagcgacag
    10801 gttatcgagc ttaagaagcg gcataagagg cctttgatgg ggtaaattaa gccgcgcatc
    10861 ttgccgcaga ccgcccttaa aggccagaaa taggcttaaa tacttgcgat tgagcactgc
    10921 ctggtcgaac cttttatggc agcatgtatc cttagtcatc gctttgcccg gaagtacgac
    10981 agcctatgcg cctctaccgt ttgctttgtt tgctgattgc ctccctttgc
//
LOCUS       DAOQGS010000060        11011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2768_length_11011_cov_4.566083, whole genome shotgun sequence.
ACCESSION   DAOQGS010000060 DAOQGS010000000
VERSION     DAOQGS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_11011_cov_4.566083"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5474)
                     /locus_tag="PKZ52_03665"
     CDS             complement(<1..5474)
                     /locus_tag="PKZ52_03665"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNG58898.1"
                     /translation="MKNSQNPSAVFNLATTPAGSLSLALCVAFASQGAWSATCPTRPA
                     CNVSQNNFSGNLAAGQSLCISGTFTGNINNLAAGATIYVPAGATFNPASIQNPAGSII
                     NCGSASLPSITLNTGFNFSNHGTADFKSNINWNGKGTFFNDAGAKINLRTTFQMKGNS
                     TITNDGEILSSGDFSSEPGTSITNTGYIRLTGGNFNPDGTVVNSGFVQTDEFININPN
                     SELINNCSFISQKGFNNNSPKTRNNGYIFVTGVGNNNDLVQNNKPFTQGPNAVVVGTR
                     FFNNGAITGGGKYYFTGDTRNQAAFGEDGGGINFYDTTRTSGKIFDSQAPNPHSSVTA
                     NAFVPPTTDAVVTSCSRNVAPVKPVITINIIAGDDIINAQEDDNAVAISGATTDAPNG
                     SLIQLEINGKTYSGTVSNNSWNILIPAADAQLFDASEIASAQVSLADNSASSDKATRT
                     VAHTTELPSINIDIVAGDDIINTTEDDTAVTVKGTTSLAENGSTVKALINGKQYSASV
                     SNNSWQFNLPAVDAQALKADEIITATVTNAAGNTSQPASRPIKHSTALPSVTIDVVAG
                     DDIINAQEDDTNVVISGTSQLAENGSSVSFTLNGKNYTATVNNNTWSTSIPAADAQAL
                     RPSETITARVTNLAGDTSLPANRTIAHNFIIPTLSINTIAGDDIINLTEDNSAVTISG
                     TTTNVENGQTVTLQVNGKNYSAQVNNNIWVTNMPAADAQALAASNIVNGQVVNLAGNS
                     ALANRSVKHSLAEPSVDIDLIAGDDIINTEEDNNPVLISGSTTLVENGQSVKVTLNGK
                     NYSATVNNNSWQFFLPAVDAQALRANERVTATATNLAGDVSEPASRDIQHSQANPSIK
                     INTVAGDDIINSTEDDLPVIISGTTTLVENGRSLSLVINGKNYSALVNNNAWSVAISA
                     QDAQALDASEIISADVSNNAGDKASATRSISHSTQTPSISINTIAGDDIINASEDDSA
                     VIISGTTSLVENGAVVAITLNGKNYTATVNNNLWSMSLPALDAQALGALDKVVARVVN
                     NAGDEANAERPLSHSGTAPTISINIIAGDDIINTTEDDAAVVISGKTTLIEDGQKVTL
                     IINGKTYEAVVTNNTWSTQMPATDAQALKAAEKVTASTNNKAGDKALGERAINHSLIA
                     PTISISPIAGDDIINATEDDAPVLISGTTTLVEDGATLTVTVNGKNYSALVSNNSWSI
                     SMPALDAQALSASEIVKAEVSNKAGDKATAERAISHAAIAPTLSINAVAGDDIINASE
                     DDAPVLISGKTTSVEDGAKLSLTLNGKNYEAVVNNNAWSISLPASDAQALDASELVAA
                     KVSNKAGDLAEASRTISHSGTAPSIIINVIAGDDIINTSEDDASVVISGTTTLVEDGQ
                     AVQLSINGKNYLAIVTNNTWSTAISPADAQALKAAEIVSAQVTNKAGDKADATRSISH
                     SVSAPSISISPIAGDDIINTSEDDAAVVVSGKTTLVEDGAKVVLTINGKQYEAIVSNN
                     NWSLLIPAIDAQALDATELVSAQVINKAGDKASTERTITHSGTAPTISINTVAGDDII
                     NTLEDDNSVTISGTTSLVEDGRAVELSLNGKNYIALVTNNSWSTDISAADAQALKAAE
                     TISAKVSNKAGDSANTNRAITHSLAAPTISINPIAGDDIINAAEDDQPVTISGKTTLV
                     EDGAKVVISINGKTYEATVSNNSWAIALPAADAQALDASETVNAQVINKAGDKANTER
                     ALTHSGTAPTLSINIVAGDDIINTTEDDSSVTISGVTTLVEDGQAVQLTLNGKNYLAV
                     VNNNQWSTAISPSDAQALNAAEKIAATVNNKAGDSANADRA"
     gene            complement(5783..6028)
                     /locus_tag="PKZ52_03670"
     CDS             complement(5783..6028)
                     /locus_tag="PKZ52_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58899.1"
                     /translation="MKRNSKLKITALNKLALAAGIVLLCGCDSRNSLKHLDSQKLKAL
                     ARDCKVAPPSAPGQIIACQNIERECTRRADKGLGACL"
     gene            complement(5988..6719)
                     /gene="phoU"
                     /locus_tag="PKZ52_03675"
     CDS             complement(5988..6719)
                     /gene="phoU"
                     /locus_tag="PKZ52_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNG58900.1"
                     /translation="MDNLHIDRHISQQFNADLEHLRTHLLELGGIVEEQVANAVKALE
                     TADSALAEQVLQTETEVDQREISLDHECTLILARRQPAASDLRLVLMVTKTTHDLERM
                     GDEADKIAKMALALNEDGASPRGYIELRHIGSNVLKLVNMALDAFARYDAQMAVRVVR
                     DDNQVDLEYRSALRELITYMMEDPRSISRVMNILWALRALERIGDHARNIAENVIYLV
                     HGMDVRHISLDEIEKKLEAENHGAE"
     gene            complement(6745..7548)
                     /gene="pstB"
                     /locus_tag="PKZ52_03680"
     CDS             complement(6745..7548)
                     /gene="pstB"
                     /locus_tag="PKZ52_03680"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNG58901.1"
                     /translation="MKTKREGSTVGKPFVDAAKLAVRGVNVFYGAKQAVFGVDLDIAK
                     NQVIAMIGPSGCGKSTFLRCLNRMNDTIESCRIEGSICLDGEDIYESKLDVVPLRARV
                     GMVFQKPNPFPKSIYDNVAYGPKIHGLASRRADLDEIVETSLRRAGLWEEVKDRLDAP
                     GTGLSGGQQQRLCIARTIAVSPEVILMDEPCSALDPIATAKIEELIAELSQNFTIAIV
                     THSMQQAARVSDRTAYFHLGKLIEVNETQKVFTNPDHELTEAYITGRIG"
     gene            complement(7670..8950)
                     /gene="pstA"
                     /locus_tag="PKZ52_03685"
     CDS             complement(7670..8950)
                     /gene="pstA"
                     /locus_tag="PKZ52_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNG58902.1"
                     /translation="MNNSPSKQAQLVAKSLAKRYSAERRFKIYGVVSIAVGLLALLCL
                     FIGIISSGIGGFTQTKIRLQLELNAEELQITNPKDPEQIKAADFQPVVRKALSERFPE
                     VEGREAKRSLYSMLSMGAPAEVQAKVIANPSLLGSGIEMWLTADDDVDTYYKSIGGKT
                     FTDRLSEKEIAWLQDLITKGELKRHFNWSFFTSGDSAEPEMAGILGTLLGSIFTLSVT
                     LALAFPIGVAAAIYLEEYAPRNKFTDFIEVNINNLAAVPSVIFGLLGLAIFINLFHLP
                     RSVPLVGGLVLTLITLPTIIISSRAAIKSVPPSIREAALGIGASKMQMILHHVLPLAM
                     PGMLTGAIIGMAHALGETAPLLMIGMVAFIMDTPGGVMSPATVLPVQIFLWSDSPERA
                     FVEKTSAAIMVLLVFLICMNTLAVWLRKRLERRW"
     gene            complement(8943..10346)
                     /gene="pstC"
                     /locus_tag="PKZ52_03690"
     CDS             complement(8943..10346)
                     /gene="pstC"
                     /locus_tag="PKZ52_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNG58903.1"
                     /translation="MNSASLLILLGLMAAGAFVLGRGKSLALVRQGDGSLGKLNSLPF
                     YYGTLTALWCIIPSALLVLVWNLFDSSLIEHLVLKPLPASIVGDNGERAALVYNQILN
                     LAAGHLESEQIDPQLLAAVSHLNQLRTTNTWAMASAGLCVGIFSALLVWLRIAPQLKA
                     RAQVEVIFRWALLVCASLAVLTTVGIILSVLFESIRFFKHVSVFNFLFGLEWSPQMAI
                     RADQTGSSGSFGAVPLFLGTLLIAALALLVAVPTGLMSAIYLSEYASRRTRSIVKPAL
                     EVLAGVPTVVYGFFAALTVAPLVRDLGLGLGLDQWITISSESALAAGLVMGVMIIPFV
                     SSLSDDVINAVPQSLRDGALGLGSTRSEVIKQVVLPAALPGIVGSILLAASRAIGETM
                     IVVMAAGMAANMTANPFDAVTTVTVQIVTLLVGDQEFDSPKTLAAFALGLVLFAVTLC
                     LNYLALHIVRKYREQYE"
     gene            complement(10436..>11011)
                     /locus_tag="PKZ52_03695"
     CDS             complement(10436..>11011)
                     /locus_tag="PKZ52_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNG58904.1"
                     /translation="WKDVNGALPATAISVLGPPPTSGTRDSFVELAMEGGCNSFPFLK
                     ALKDTKKEEYQAACGAIREDGAYIEAGENDNLIVRKLEENVNALGIFGYSFLEQNADK
                     VQGSKIGGKEPTFETIADGSYVVARPLFIYVKKQHIGMVPGIKEFIAEFTSEAAWGDE
                     GYLSEKGLIPLPKKDRTAAAANAQALEAMKL"
BASE COUNT         2419 a         2698 c         3053 g         2841 t
ORIGIN      
        1 gcgcgatctg cattggcgct atcgcccgct ttgttattga ctgtggcggc aattttttcg
       61 gcggcattta aggcctgggc gtcgctgggg ctaatggcag tagaccactg attattattc
      121 actaccgcca aatagttttt gccattcagt gttaattgca ccgcctgacc atcttccacc
      181 aaagtggtta caccgcttat ggtgactgag ctgtcatctt ctgtggtgtt aataatgtca
      241 tcgcccgcaa caatattaat gctaagggtt ggcgctgtgc cggagtgcgt tagggcgcgc
      301 tcggtgttgg ctttatctcc ggctttatta atcacttggg catttaccgt ttcgctggca
      361 tcaagcgctt gggcatccgc cgcaggcagg gctatggccc agctattatt gctcaccgtg
      421 gcttcgtagg ttttgccatt aatgctaata acaaccttag cgccgtcttc caccaaagtg
      481 gttttaccgc tgatggtgac gggttggtca tcttcagcag cattaataat atcgtcgcca
      541 gcaatggggt tgatgctaat ggtgggggct gcaaggctgt gcgtaatagc acggttggtg
      601 ttggcactgt cccccgcttt attgctcacc ttggcgctaa ttgtttccgc cgcttttaag
      661 gcttgtgcat cggcagcgga tatatcggtg gaccaactat tattagtcac cagggcaata
      721 taatttttgc cattgaggct taattccacc gcccggccat cttccactag gctcgtggta
      781 ccgctgatgg tgacggaatt atcgtcttcg agggtattaa taatatcgtc gcctgccacc
      841 gtattaatac tgatggtagg tgcggtgccc gagtgggtaa tggtgcgctc ggtgctggct
      901 ttatcaccgg ctttattaat cacttgggca ctgactaatt cagtggcatc taaggcttgc
      961 gcatctatgg ccggaattaa cagtgaccaa ttgttattgc tcacaattgc ttcgtattgt
     1021 ttgccgttaa tggtgagtac aactttcgcg ccgtcttcca ctaaagtagt tttaccactt
     1081 acgacaacgg ctgcgtcatc ttcgctggta ttaataatat cgtccccggc gatagggctg
     1141 atactaatgc tgggtgcgct cacactgtgg ctaatagagc gtgttgcatc ggctttatct
     1201 ccggctttat tagtaacctg tgcgctgaca atttccgcgg cttttaatgc ttgtgcgtcg
     1261 gcggggctga tggcggtaga ccaagtgttg ttggtgacta tggctaaata atttttgcca
     1321 ttaatactga gttgaaccgc ttggccatct tccactaatg tcgtggtgcc gctaatcacc
     1381 accgatgcat catcttcgct ggtattaata atatcgtcac cggcaataac attaataata
     1441 atgctcggtg cggtgcctga gtgactaatg gtgcggctgg cttcggccag atcaccggct
     1501 ttattgctca ctttggcggc aaccaattca ctggcatcta aggcttgggc gtcgctggca
     1561 ggcagggaaa ttgaccaggc attattatta actaccgcct cataattttt gccattgagc
     1621 gttaggctta attttgcgcc gtcttccacc gaggtggttt tgccgctaat aagtaccggt
     1681 gcatcatctt cactggcatt aataatatcg tcgcccgcaa ctgcgttaat gctcagggtg
     1741 ggggcgatgg cggcatggct aattgcacgc tcagcggtgg ccttatcgcc cgctttattg
     1801 ctcacttctg cttttactat ttcgctggcg ctcaacgcct gagcgtccag ggcgggcatt
     1861 gaaatagacc agctattatt gctcaccaag gcagaataat ttttgccatt taccgtaacc
     1921 gttaaggtcg cgccatcttc cactaaagtg gttgtgccgc taattaatac tggagcgtca
     1981 tcttctgtgg cattaattat gtcatcgccc gcaatggggc tgatgctaat agtgggggca
     2041 attaaactgt ggttaatggc gcgctcgcct aaggctttat cgccggcctt attatttgtg
     2101 ctggcagtaa ctttttccgc tgcttttaat gcctgggcat ccgtggccgg catttgggtt
     2161 gaccaagtat tattagttac aacagcttcg taggttttac cgttaataat taaggtaact
     2221 ttttggccgt cttcaatcag ggtggtttta ccgctaatga ctacagctgc atcatcttcc
     2281 gtagtattaa taatgtcgtc accggcgata atattaatgc tgatggtagg cgcggtgccg
     2341 gagtggctca ggggccgctc agcgtttgct tcatcaccgg cattattcac cacacgggca
     2401 acaactttat ccagagcgcc gagggcttgt gcatccagtg caggcaggct catggaccaa
     2461 agattattat tcaccgtggc cgtgtaattt ttaccgttta aagtaatggc aacaaccgcg
     2521 ccgttttcca ccaggctagt cgtaccgcta ataatcacgg cagaatcgtc ttcgctggca
     2581 ttaataatat cgtcgcctgc gatggtatta atgctgatgc tgggggtttg tgtgctgtgg
     2641 ctgatggagc gcgtggcgct ggctttatca ccagcgttat tactcacatc ggcgctaata
     2701 atttcgctgg catccagggc ctgggcatcc tgggcggaaa tagctaccga ccaggcatta
     2761 ttattaacca gggcggaata atttttacca ttaataacga ggctaaggct gcgaccattt
     2821 tccaccaagg tggtggtgcc gctgataata accggcaggt catcttcagt gctgttaata
     2881 atgtcgtccc cggccaccgt attaatttta atgctggggt tggcttgtga gtgctgaata
     2941 tcgcgggagg caggctctga aacatcgccc gctaaattag tggcagtagc cgttacccgc
     3001 tcattggcgc gcagggcttg agcatcaact gcaggcagga aaaattgcca gctattgtta
     3061 ttaaccgtgg cgctgtaatt tttgccatta agggtaactt ttacgctttg gccattttcc
     3121 accaatgtgg tggagccgga aattaatacc gggttgttgt cttcttcggt attaataata
     3181 tcgtccccgg caatcaggtc gatatccaca ctcggctcag ccaagctgtg ttttacgctg
     3241 cgattggcaa gagcgctatt gcccgccaga tttaccacct gaccattaac gatgttgctt
     3301 gcggccaggg cttgggcatc ggcagcgggc atgttggtaa cccaaatatt attgttcacc
     3361 tgggcagaat aatttttacc gtttacctgc agcgtaactg tttggccatt ttccacatta
     3421 gtggtggtgc cgctaatggt taccgcgctg ttatcttcgg ttaaattaat aatatcgtcc
     3481 ccggcaatgg tattaatgct cagggtggga ataataaaat tgtgggcgat ggtgcgattg
     3541 gcgggcagtg aagtgtcacc ggctaaattg gtaacacggg cggtaatggt ttcgctgggg
     3601 cgcagggcct gtgcatcggc ggcgggaatg ctggtggacc aggtattgtt attcaccgtg
     3661 gccgtgtaat ttttaccatt gagggtaaaa cttacgctgc tgccattttc ggctaattgg
     3721 ctggtgccgc taattacgac attggtgtcg tcttcctggg cattaataat gtcatcgcct
     3781 gccactacat caatagtaac gcttggtaga gcagtgctgt gtttaatggg gcggctggcc
     3841 ggttgcgatg tgttgcccgc agcgttggtt acggtggcgg taataatttc atcggcttta
     3901 agtgcttggg catcgacggc gggtaaatta aattgccagc tattattgct tacgctggcg
     3961 ctatattgtt tgccgttaat cagcgcctta acggttgagc cattttcagc aaggctggtg
     4021 gtgcctttaa cagttaccgc cgtgtcatct tccgtggtgt taataatgtc atcgcccgcc
     4081 actatatcaa tattaatgct cggtaattct gtggtgtgtg caactgtgcg ggtggcttta
     4141 tcgctgctgg cggaattatc cgccaagctc acttgggcgc tggcaatttc gctggcatcg
     4201 aataattggg cgtcggcggc gggaattaaa atattccaac tattgttgct tacagtgccg
     4261 gaataggttt tgccattaat ctccagttga attaacgagc cgttgggagc atcggtggtg
     4321 gcgccgctga tggccacggc gttgtcatct tcctgggcgt tgatgatgtc atcgcccgca
     4381 ataatattaa tagtaataac gggtttaacc ggtgctacat tgcgcgaaca gctggtaacc
     4441 accgcatcgg tagtcggtgg tacaaatgca ttggcggtta cgctggagtg gggattgggg
     4501 gcttgggaat caaaaatttt cccgctggtg cgggtggtat cgtaaaaatt aatgccgccg
     4561 ccatcttcac caaaggctgc ttggttgcgg gtatcgccgg taaaataata tttaccgccg
     4621 ccggtaatgg cgccgttgtt aaaaaaccgc gtgcccacca ccactgcatt ggggccttgg
     4681 gtaaagggtt tgttgttttg caccaagtcg ttattgttgc ccacgccggt tacaaaaata
     4741 taaccattgt tgcgggtttt ggggctgttg ttgttaaagc ctttttgcga gataaaactg
     4801 cagttattaa ttaattcgga attggggttg atattaataa attcgtcggt ttgtacaaaa
     4861 cctgagttca ccaccgtgcc gtcggggtta aaattgccgc cggttaagcg aatataaccg
     4921 gtgttggtga tgctggtgcc gggctcgctg gaaaagtcac cgctacttaa aatttccccg
     4981 tcgttggtaa tcgttgaatt acctttcatt tgaaaggtgg tgcgcaggtt tattttggct
     5041 ccggcatcgt taaaaaaagt gcccttgccg ttccagttaa tattgctttt aaaatccgct
     5101 gtgccgtggt tagaaaaatt aaaacccgta tttaaggtaa tagaaggtag gctggcgctg
     5161 ccgcagttga taatactgcc cgctgggttt tgaatactcg ccgggttaaa ggtggccccg
     5221 gcgggtacat aaatagtcgc ccctgctgcc aaattattaa tattgccggt aaaggtgccg
     5281 gagatacaca ggctctgccc ggcagctaaa ttgccggaga aattattttg cgacacatta
     5341 caggcgggcc gagtagggca ggtggccgac caggcgcctt ggcttgcgaa ggcaacacac
     5401 agggcgagac ttaaggatcc ggcaggggtg gtggcaaggt taaaaacggc agagggattt
     5461 tgcgaattct tcacgggggg ttcctaaaat gtatacgcgc aagtgtggat ttactacggt
     5521 tgggcgggcg gaatctactt gaactggggt gaataaacaa ctaatgggtc acatgattac
     5581 taggctttta ttaccggcgc aatacagcag ataaagtgtg atcagattca tgttatgtgc
     5641 ttgtgatggg ttgattcaac gcaatcccat gcctagcggg ttaaataccg cgcgggggct
     5701 ggctgggggt taatctgtga tctacgtcgc ggcggcgttg cggacggtag ctgggttgat
     5761 tgccgctgtt tgtcggttga ctttacagac aggcgcccag gcccttgtct gcacgccgtg
     5821 tgcattcacg ttcgatgttc tggcaagcaa taatctgtcc aggggcgctg ggcggtgcaa
     5881 ctttgcagtc gcgggcgagg gctttgagtt tttggctatc caggtgttta aggctgttgc
     5941 ggctgtcgca gccgcacagc aacacaatac ctgccgccag ggctaattta ttcagcgccg
     6001 tgattttcag cttcgagttt cttttcaatt tcatcgaggg aaatatgccg cacgtccatg
     6061 ccgtggacca ggtaaataac gttctcggcg atgttgcggg cgtgatcacc aatacgctcc
     6121 agcgcgcgca gggcccataa aatattcatc acccgggaaa tcgagcgcgg atcttccatc
     6181 atgtaggtaa ttaattcgcg cagagcggag cggtactcca aatccacctg gttatcatcg
     6241 cgcactacgc gcaccgccat ttgggcgtcg tagcgggcga aggcatctaa ggccatattg
     6301 acgagtttta acacgttgga gcctatgtgg cgcagctcga tgtagccgcg cggggaggcg
     6361 ccgtcttcat tcaaggccag ggccatcttg gcgattttgt ccgcctcatc gcccatgcgc
     6421 tccaggtcgt gggtggtttt ggtaaccatt aataccaggc gcaaatccga agcggcgggc
     6481 tggcggcggg cgagaatcag ggtgcattcg tggtccaggg aaatctcccg ctggtccacc
     6541 tctgtctcgg tttgtaatac ctgctcggcc agggcactgt cggcggtttc cagggctttc
     6601 acagcattgg ccacctgctc ctcgacaatt ccacccagct ctaacaggtg agtgcgcagg
     6661 tgctcaagat ccgcgttaaa ttgctgggag atatgtcgat cgatatgtag gttatccatg
     6721 gtgtcaggtc tcaattcgca caggctagcc gatgcggccg gtgatatagg cttcagtcaa
     6781 ttcgtgatcg gggttggtaa atactttttg ggtttcatta acctcaatga gcttacccaa
     6841 atgaaagtaa gcggtgcggt cggaaacccg cgcggcctgc tgcatggagt gcgtcactat
     6901 ggcgatggtg aaattctggc tcaattcggc aattaattct tctatttttg cggtggcgat
     6961 ggggtcgagg gcggagcagg gctcgtccat caaaatcact tccgggctta cggcgatggt
     7021 gcgggcgata cacaagcgct gctgttgacc gccggagagg cccgtgccgg gtgcgtctaa
     7081 gcggtctttg acctcttccc acaggcccgc ccggcgcaaa ctggtttcga cgatttcgtc
     7141 taaatcggcg cggcggctgg cgaggccgtg aattttaggg ccgtaggcga cattgtcgta
     7201 aatgcttttg gggaagggat tgggtttttg aaacaccatg ccgacgcggg cccgcagggg
     7261 cactacgtct aatttggatt cgtaaatatc ctcgccgtcc aaacaaatac tgccttcaat
     7321 gcggcagctt tcgatggtgt cgttcatgcg gtttaagcag cgcaaaaagg tagatttgcc
     7381 gcagccggag gggccgatca tagcgatcac ttggtttttc gctatatcca aatccacgcc
     7441 aaaaaccgct tgttttgcgc cgtaaaaaac atttaccccg cgcactgcga gttttgcggc
     7501 atcaacaaag ggttttccaa cggtgctgcc ttcgcgcttg gtcttcatct tcggttttaa
     7561 ctcattcaag gttgcattgg tgggcgtgat cggcagcggt tcagcctgcg cgggcgcttt
     7621 gttcataaca aggggttcaa gtacttcact cataatgcac tcctaataat taccagcgcc
     7681 gttccagccg cttgcgcagc cacaccgcca gtgtattcat gcaaattaag aagaccaaaa
     7741 gcaccataat ggccgcggag gttttttcca caaaggcgcg ctcgggactg tcggaccaca
     7801 aaaatatttg taccggtaat accgtggcgg gcgacatcac accgccgggg gtatccataa
     7861 taaacgccac catgccgatc atcagcagcg gtgcggtttc gcccagcgcg tgggccatac
     7921 cgataatagc gccggtgagc atgcctggca ttgctagagg taacacatgg tgcaaaatca
     7981 tttgcatttt ggaggcgccg atgcccaagg ccgcttcgcg aatgctcggc ggcacggatt
     8041 taatggcggc gcggctggaa ataataatgg tgggcagggt aattaacgtg agcaccaggc
     8101 cgccgaccaa aggcactgag cggggcaggt gaaataaatt aataaaaatg gccaggccca
     8161 agaggccgaa aatcaccgag ggcaccgccg ccaaattatt aatattgacc tcgataaaat
     8221 cggtgaattt attgcgcggg gcgtattcct ctaaataaat cgcggcggcc acaccgatgg
     8281 gaaatgccag cgctaaagtg accgacaggg taaaaatgct gcccagtaag gtgcctaaaa
     8341 taccggccat ttccggctcg gcggaatccc cagaggtaaa aaagctccag ttaaagtgcc
     8401 gctttaattc gcctttggta attaaatcct gcagccaggc gatttctttt tcgctcaggc
     8461 gatcggtaaa tgttttaccg ccaatacttt tgtaataggt atccacatcg tcatcggcgg
     8521 ttagccacat ctcgatgcca ctgccgagca aactggggtt ggcgatgact tttgcctgca
     8581 cttccgccgg ggcgcccatg ctcagcatgg aatacagtga gcgtttggcc tcgcggccct
     8641 ccacctcggg gaaacgctca ctgagtgctt tgcgcaccac aggttgaaag tcggcggctt
     8701 taatttgttc tgggtctttg ggattggtga tctgcaattc ttcagcattt aattccaact
     8761 gcaggcggat tttagtttgc gtaaaaccgc ctatgccgct gctgataatg ccgatgaaca
     8821 aacacagcag ggccagcaag cccacggcaa tggacaccac gccataaatt ttaaaacgcc
     8881 tctccgcgct gtagcgcttg gccaggcttt tggcgactaa ttgcgcctgt ttagaaggac
     8941 tgttattcat actgttctcg gtatttgcgc actatgtgca gcgccaggta attcaaacaa
     9001 agtgtcacag caaacagcac caaacctagg gcaaatgccg ccagggtttt ggggctgtca
     9061 aattcttggt cgcccaccaa gagggtgaca atttgcaccg taaccgtggt gaccgcatcg
     9121 aaggggttgg cggtcatatt ggcggccata ccggcggcca tcaccacaat catggtctcg
     9181 ccaatggcgc gggaggcggc caataaaatg gagcctacga tcccgggcag ggcggcgggc
     9241 aacaccactt gtttgataac ttctgagcgc gtggagccca ggcccagggc gccatcgcgc
     9301 agggactggg gcacggcgtt gataacatcg tcggacaggg acgacacaaa cggaataatc
     9361 atcaccccca tcaccaggcc tgcggccagg gcgctctctg agctgatggt gatccactga
     9421 tcaagcccga ggccgaggcc taaatcgcgc accaagggtg ccaccgtaag ggcggcgaaa
     9481 aagccgtaca ccacggtggg caccccggcc aatacttcga gggcgggttt gacaatactg
     9541 cgggtgcggc ggctggcgta ttcggagagg taaatagcgg acataagccc ggtgggtacc
     9601 gccaccaaca gggccagggc ggcgatcagc agggtgccaa ggaacagggg cacggcgccg
     9661 aagctgccgg agctgccggt ttgatcggcg cggatggcca tctgcgggct ccactccagg
     9721 ccgaataaaa agttaaacac cgacacgtgt ttaaaaaagc ggatggattc aaacagtacc
     9781 gaaagaataa tgcccacggt ggtgagcacc gcaaggcttg cacacaccag cagtgcccag
     9841 cggaaaatca cctccacctg ggcgcgggct ttcagttgcg gcgcgatgcg cagccagact
     9901 aacagggcgg aaaagatgcc cacacacaac ccggcggaag ccatggccca ggtgttggtg
     9961 gtgcgcagtt ggtttaaatg gctaacggcg gccaacagct gcgggtcgat ctgctcgctc
    10021 tcgaggtggc ccgccgccag gttcaatatt tggttgtaca ccagggcggc gcgctcgccg
    10081 ttgtcgccca ctatgctggc gggcagaggt ttgagcacca agtgttcaat caggctgcta
    10141 tcaaataggt tccacaccag caccaagagg gcgctgggga tgatgcacca tagggcggtg
    10201 agggtgccgt agtaaaaggg cagggaattg agcttgccta aggagccgtc cccctgccgt
    10261 accagggcta aggatttacc gcgccctaac acaaaggcgc ctgcggccat taggcccagc
    10321 agaattaaca gggaggctga attcatagtt gaagtgcgct tgttttaagg ttaattcaca
    10381 acacaaaagc ccggaagatc cgggcttttg tgtttttcgg gttacatacc gatcactaaa
    10441 gtttcatggc ttcaagtgct tgagcattag cggcggccgc tgtgcggtct ttcttcggca
    10501 gcgggatcag gcctttttca gacagataac cctcatcacc ccaggccgct tcgctggtga
    10561 actcagcgat aaattcttta ataccgggta ccatgccgat gtgttgtttt ttcacgtaga
    10621 taaacaacgg gcgggctacc acatagctgc catcggcgat ggtttcaaag gttggctctt
    10681 tgccgccaat tttggaaccc tgcaccttgt cggcgttttg ctctaggaag ctgtaaccga
    10741 aaatacccag ggcatttacg ttttcttcca gtttgcgcac gatcaggttg tcattttcgc
    10801 cggcttcgat ataggcgcca tcttcacgga tagcaccaca ggccgcttgg tattcttctt
    10861 tcttagtatc tttgagggct tttaagaacg gaaagctgtt acagccgcct tccattgcca
    10921 actcaacgaa ggagtcgcga gtgccggaag taggtggggg gcctaacacg ctgatggcgg
    10981 tggcaggcag ggcgccgttc acgtctttcc a
//
LOCUS       DAOQGS010000061        11008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2771_length_11008_cov_4.096960, whole genome shotgun sequence.
ACCESSION   DAOQGS010000061 DAOQGS010000000
VERSION     DAOQGS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_11008_cov_4.096960"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKZ52_03700"
     CDS             complement(<1..259)
                     /locus_tag="PKZ52_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG58905.1"
                     /translation="MHRSLLHSSLLSTAAFANYAASFSVGALYALVVPTLSLHLAQAY
                     QVSAWWIGVFFIGKALASIFFAQLIARRSDRLKDRRPLIAAA"
     gene            complement(259..1050)
                     /locus_tag="PKZ52_03705"
     CDS             complement(259..1050)
                     /locus_tag="PKZ52_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNG58906.1"
                     /translation="MVKPSGPKGDSAEYREKKRLLARMLTIYGRNPVLEALQDERIKV
                     FRLHLADSNKPNAMLSDMQRLAKQKGAEVLWHSKQALSRISKNSQQDQGVALDVITEG
                     FCHEDEWLTALPEHFELMAVDGVTNPQNLGMIIRSLCASPLAGLLLPEKGCAKLDALV
                     IKASAGTLFRAPIIHTADLAACLTRLKQQGCKVFGLDAEGSSTIGSFQAPTRCVLVMG
                     NESTGLSPQVKALCDGFISIPMQRGVESLNVSIAASLIAFRSLLN"
     gene            complement(1043..1813)
                     /locus_tag="PKZ52_03710"
     CDS             complement(1043..1813)
                     /locus_tag="PKZ52_03710"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNG58907.1"
                     /translation="MAIALSVNLNKIALLRNSRSGNYPDPMAFARIALTEGAKGLTVH
                     PRPDQRHVRPEDVYNCRALLSAYSDCEFNIEGNPFAAPMGSYPGLLPLVEATRPEQCT
                     LVPDTNSQLTSDHGFDLRKDGERLKPVLAQLHNWGVRVSLFMDPDIEQIRLAAALGAD
                     RIELYTGPFAEAFLHQAPNLAELIDRYAAASAEAKALGMGVNAGHDLNLENLPSLYAL
                     PCLQEVSIGHALTVDALIMGFGPAVAAYVALCDGYPHG"
     gene            complement(1908..2540)
                     /locus_tag="PKZ52_03715"
     CDS             complement(1908..2540)
                     /locus_tag="PKZ52_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HNG58908.1"
                     /translation="MKRLKTILAPWVGALGLLWLGLQAAQAETLYISDVCYTSLRAGQ
                     TNDSKVVHSGLKTGTPVTLLEAPAESDWSRVKTDTGQEGWVRRQFLLTGPTAQMQLDK
                     LKTDTANRPAPVDPTAEVPAACQSYAAELASLKALSSDAVNLNKRYQDLLAEHEMAKT
                     TLDSLRAENERLKGSTQHTQWIYGGILVAAGILLTLLIQALKPSRRSEWV"
     gene            complement(2559..2858)
                     /locus_tag="PKZ52_03720"
     CDS             complement(2559..2858)
                     /locus_tag="PKZ52_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNG58909.1"
                     /translation="MYYAIISQDVPDSLPLRLSARPAHLARLQALAADNRLLIAGPHP
                     AIDSASPGEAGFSGSLVVAEFTSLAEAQRWADADPYITAGVYAQVVVKPFLKVLP"
     gene            complement(2860..3585)
                     /locus_tag="PKZ52_03725"
     CDS             complement(2860..3585)
                     /locus_tag="PKZ52_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HNG58910.1"
                     /translation="MAAAPAPQENMLVNLLINVAAPSIILSKFSGEHSLGPTWGLIVA
                     LAFPIGYGLWDFIRRRKVNFFSAIGVFSTFLTGGMTLLKLPPEYVAIKEASIPLLFGL
                     ATLGSLYTPFPLVRTFLYNEKVLQVERVNHALKHYGTEQAFERTLVVASWMLAGSFFL
                     SAVLNYGLAKYLLVSAPGTEAYNAELGKMTALSFPVITIPSMLVLVGALWFLLNRIKS
                     LTHLKLEEIFNDGQADSKTTADM"
     gene            3646..4488
                     /locus_tag="PKZ52_03730"
     CDS             3646..4488
                     /locus_tag="PKZ52_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNG58911.1"
                     /translation="MIYDLHTHSNASDGFLSPADLVARAQAKGVNALALTDHDPLAGF
                     SQAAAAAQQADISLIPGIEFSTQWLGRSIHIVGLGFNPAAASITQGIKRQATSRLERA
                     QTIGEKLAKAGVPDAYAGAQALAGDAVLGRPHFAQYLVACGAVKDVASAFKRYLGAGK
                     MGDVKQLWPGVEEAVEWITAAQGVAVLAHPDKYDMTRSKLCQLLGQFREAGGQALEVI
                     SGKQVNTLTDKLARLAGQFSLAASCGSDFHVPNQPWQELGAFGKLPDFCQPVWQLLPG
                     HLSS"
     gene            4491..5111
                     /locus_tag="PKZ52_03735"
     CDS             4491..5111
                     /locus_tag="PKZ52_03735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNG58912.1"
                     /translation="MALLLQIHPDNPQERLIKQAVEIIRRGGLVVYPTDSAYALGCHI
                     GDKLALDRIRAIRQLDKHHNFTLVCKDLSELATYARVNNSAFRLLKNHTPGPYTFILD
                     ATGEVPRRLMHPKRKTIGLRVPSHPITQALLAELGEPIMTSSLIMPGEELPLTDPYDI
                     RELLDHHVELVIDGGFCGLDPTTVVDLTEGEPVLVRQGRGDASAFT"
     gene            5121..5933
                     /locus_tag="PKZ52_03740"
     CDS             5121..5933
                     /locus_tag="PKZ52_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNG58913.1"
                     /translation="MHSALPSEPAAKPLAIVKGEAWQKLPKDLYIPPEALAVSLSQFE
                     GPLDLLLYLIRKHNLDILDIPVLSITQQYLAYIEAIDAARVELAADYLVMAAYLVEIK
                     SRLLLPRPPVAEDWGDVEDDPRAELVAKLLEYQQLQAVAEQLDALPRWQRDILPVQVY
                     IPPTQWPTRWVPLTASELAQAWSAVAERHRLNGQHVIEAEVFSTRARMGQLLIRLRSA
                     NAWQPFTGLLIAAEGRAGVVVTFIALLELIKEGYVQAEQTADELRLCAHEPN"
     gene            5920..6600
                     /gene="scpB"
                     /locus_tag="PKZ52_03745"
     CDS             5920..6600
                     /gene="scpB"
                     /locus_tag="PKZ52_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNG58914.1"
                     /translation="MSPTSSELTSQSSTAADGKSSAASAGPKSLVEAAIFAAVEPIPT
                     RQLLKLWGEAEDESHRQQLQEILSALQADYQGRGVELVQVASGWRFQVPKPVAQLLEP
                     LYQEKPKRLSRVILETLAIIAHRQPITRAEIEALRGVAVSAEVLKNLIERQWICSLGP
                     KEIPGRPMQYGTTDEFLDYFNLQSLAQLPSLLPPAPAEPNALSDISAGDTAQLINDPR
                     PDENQPEP"
     gene            6610..7857
                     /locus_tag="PKZ52_03750"
     CDS             6610..7857
                     /locus_tag="PKZ52_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNG58915.1"
                     /translation="MTDEKLQKVLATAGFGSRREMERVIDAGLVTVNGVTATLGSRVK
                     AGDKLIIDGHRYQVPDRKETQDELRVLLYNKPEGEICSRNDPEGRPTVYDRLPPLSTG
                     RWISVGRLDFNTSGLLLFTNNGELANKLMHPSSGIDREYLVRVQGEVSKEQLQQLRDG
                     VQLDDGLAKFTDITIGGGKGANTWFYCTLMEGKNREVRRLWEAQGCRVSRLKRVRYGQ
                     ILIPSYVRMGQWVELGLAETTLLCDAAGIKPPAKKTLTRNEFLQRERREQRLRASPGK
                     PVAVKSRAHRSRAEREAFIARASSDPHAKPGIQGRADTAKTKTRPEETKPAKGGAGRA
                     KPAGKTGKETGSARVEAGKTTAGPRTASTEARSTQTDKPRSVKAPQSKSASAARSASP
                     KPNGQSQSKPAAPRPRSPRSKKD"
     gene            8397..9647
                     /locus_tag="PKZ52_03755"
     CDS             8397..9647
                     /locus_tag="PKZ52_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNG58916.1"
                     /translation="MAEVKQLVLGAAVAAALGSTAAYAGFSVSGTQLLEGNGRAFVMR
                     GINHAHTWYSTRTPKAMADIAATGANTVRVVLSDGQQWSKTPASEVANIIKLCKDNKL
                     ICMLEVHDGTGYGEQSAAGTVAKIAQYWVEIAPVLKGQEDYVLVNIANEPFGSGVAAS
                     VWIDAHKAAIQTLRNAGLTHTIVVDAPAWGQDGNEAMLANAKTVAAADTRKNTIFSVH
                     MYEVYQSRSKVENYLTKFLSSNNLPIIVGEFGVENNGNQVDVESILAVAQQYKIGYLG
                     WSWSGNGSCCVPLDIVKDFNPATPSTWGTRLISGTNGIKQTSVVASIYGGVPSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSGSGRTSSSSSSSSSSSSSGSSSSSGGNAAGS
                     FDSLGLWGLCLAALGLGRRRKAVV"
     gene            complement(9779..>11008)
                     /locus_tag="PKZ52_03760"
     CDS             complement(9779..>11008)
                     /locus_tag="PKZ52_03760"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNG58917.1"
                     /translation="LAFSVTFPGRPRLQESLLRSVAFITLRWFTIASLIIFLGYATRS
                     LNLFHKDAIANWFWIGPLAQVGSHLLFRAASPWLLKLQGQSRSAVIVGMNDQGIELAK
                     RIKSNPYSNAHLVGFFEDRDPARVSQYNIWPFLGTTAQLSHFCKEHHIDLIYLSLPMA
                     TQDRILKILDDLRDTTASIYFVPDLFVTDLIQGNISQVANMPVVSVCETPFTGVNGLV
                     KRISDIVLASLILILIMPVLIAVAIAVKYSSPGPIIFKQRRYGLNGEEITVYKFRSMT
                     VCENGSEVKQAQRNDARVTRVGAFIRKTSLDELPQFINVLQGRMSIVGPRPHAVAHNE
                     LYRKQIKGYMIRHKVKPGITGWAQVNGLRGETETLDKMQARIEFDLDYLRNWSVALDV
                     KIILMTAIMMFRQPQAY"
BASE COUNT         2455 a         3134 c         3238 g         2181 t
ORIGIN      
        1 tggcggcggc gatcagcggg cggcggtctt ttaggcggtc ggagcggcgg gcgatcagtt
       61 gggcgaagaa aatactcgcc agggccttgc cgataaaaaa caccccaatc caccaggcgg
      121 atacctgata agcctgggcc aaatgcaagc tgagggtggg cactaccaag gcgtagaggg
      181 cacctacgga aaacgacgcc gcgtagttgg caaaggcggc ggtgctgagt aaagagctgt
      241 gcagaaggga gcggtgcatt agttgagtag gctgcgaaag gcgatgagac ttgcggcgat
      301 gctcacgttt aaggattcca ccccccgctg catggggatg ctaatgaacc cgtcgcagag
      361 tgccttcacc tggggggaaa gccctgtgct ttcattgccc attaccaaaa cacagcgggt
      421 tggcgcctgg aagctgccaa tggtggagct gccctcggca tccagcccga acaccttaca
      481 gccctgttgt ttcaggcgtg tgaggcaggc ggctaaatcc gctgtgtgaa taatgggggc
      541 gcgaaagagg gtgcccgcgc tggctttaat caccagggcg tcaagcttgg cgcagccttt
      601 ctccggcagc agcagcccgg ctaaggggga ggcgcacagg ctgcggataa tcatgcccag
      661 gttctgaggg ttagtaaccc cgtccactgc catcagctca aagtgctcag gcagggcggt
      721 gagccactcg tcttcgtggc aaaagccctc agtgatgaca tccagggcaa ctccctggtc
      781 ttgctggctg ttttttgaaa tccttgagag ggcctgtttg ctgtgccaaa gcacttcagc
      841 gcctttttgt ttagccaggc gttgcatatc gctcagcata gcgttgggtt tgttgctatc
      901 ggccagatgc aggcggaaca ccttgatgcg ctcatcctgc agggcttcta acaccgggtt
      961 gcggccgtaa atggtgagca tccgggcgag caggcgcttt ttctcccggt actcggcgga
     1021 gtcgcctttt gggcctgaag gtttaaccat ggggatagcc atcgcaaagg gccacatagg
     1081 cggccactgc ggggccaaag cccattatga gcgcatccac cgtcagggcg tggccgatgg
     1141 aaacctcctg taaacagggc agggcgtaga ggctgggtag gttttcaagg tttaaatcgt
     1201 ggcccgcatt tacccccatg ccgagggcct tggcctcggc gctggcggcg gcgtagcggt
     1261 cgatcaactc ggcaaggttg ggggcctggt gcaaaaaggc ttcggcgaag gggccggtgt
     1321 aaagctctat gcggtccgcc ccgagagctg cagcaaggcg aatctgctct atatccgggt
     1381 ccataaacag gctcacccgc acaccccagt tgtgcaattg ggcgagcacg ggttttagcc
     1441 gctcgccgtc cttacgcagg tcaaaaccgt ggtcagaggt gagctggctg ttggtgtccg
     1501 gcaccaatgt gcactgctct gggcgggtgg cctccactaa gggtaaaagg ccagggtagc
     1561 tgcccatggg ggcggcgaag ggattgcctt cgatgttgaa ttcacagtcg ctgtaagcgc
     1621 tgagcagtgc gcggcagttg tacacatcct ccggccgcac atggcgctgg tcgggcctcg
     1681 ggtgcaccgt caggcccttg gccccttcgg ttagggcgat gcgggcaaag gccatggggt
     1741 ctggatagtt gccgctgcgg gaattgcgca gcagagcaat tttgttgagg ttaaccgaca
     1801 gggcgatggc catggttaaa agctccagaa aaaggcggtg ctgtaaataa aaacggctcc
     1861 aaggggagcc gattggggtt actcagctgg gcagtgaggt taccggctta cacccactcc
     1921 gaacgacggc tgggtttgag ggcctggatc aacagggtca acaaaatccc cgccgccacc
     1981 agaataccgc cgtaaatcca ctgggtgtgc tgggtgctgc ccttgaggcg ctcgttctcc
     2041 gcccgcaggc tatctagggt ggttttggcc atttcatgct cggccaataa atcctggtag
     2101 cgtttattca ggttcaccgc atcgctggag agagccttaa ggctggctaa ctctgcagcg
     2161 tagctttggc aggcggcggg tacttccgct gtagggtcca cgggggccgg gcggttggcg
     2221 gtgtcggttt tgagcttatc taattgcatc tgggcggtgg ggccggtcaa taaaaactgc
     2281 ctgcgcaccc atccttcctg gccggtgtcg gttttcaccc ggctccagtc ggattccgcc
     2341 ggggcttcta gcaaggttac cggggtgccg gttttaaggc cggagtgcac cactttgctg
     2401 tcgttggttt ggcccgcccg cagggaggtg taacacacat cagagatgta cagggtctct
     2461 gcctgggcgg cctgcaggcc cagccacaat aggcccaggg cacccaccca gggtgcgagg
     2521 atggttttaa gtcgtttcac aaataagaac cttacccgct aggggagtac ttttaggaag
     2581 ggtttcacca ccacctgggc gtacacaccg gcggtgatat aagggtccgc atcggcccag
     2641 cgctgcgcct cggccaggga ggtaaattcc gccactacta aactgccact aaaacctgcc
     2701 tcccccgggc ttgcgctatc gatggccggg tgggggccag cgatcagcag ccggttgtca
     2761 gccgctagcg cctgcaggcg cgccaggtgg gcgggccgtg cactcaggcg caggggcagg
     2821 ctgtcgggta cgtcttggct gatgatggcg tagtacatgt tacatgtctg cagtggtttt
     2881 agaatcggcc tggccgtcgt taaaaatctc ttccagcttt aagtgcgtca ggcttttaat
     2941 gcggttgagc agaaaccaca gggcgcccac cagtaccagc atggagggaa tggtgatgac
     3001 cgggaaactc agcgccgtca tcttgcccag ctcggcgttg taggcttcgg taccgggggc
     3061 actgacaagc agatatttgg ccaagccgta attgagcacg gcggagagaa aaaacgagcc
     3121 cgccagcatc caggaagcca ccactagggt gcgctcgaag gcctgttcag tgccgtagtg
     3181 tttaagggca tgattcaccc gctccacctg caacactttc tcgttgtaca aaaaggtgcg
     3241 caccaggggg aagggcgtat acagggagcc taaggtggca aggccaaaca gcaggggaat
     3301 gctggcctct ttgatggcca catattcagg gggcaattta agcagcgtca tgccgccggt
     3361 taaaaaggtg ctgaacaccc cgatggcgga gaaaaaattc accttgcggc ggcggataaa
     3421 atcccagagg ccatagccga tggggaaggc cagggcaaca attaagcccc aggtagggcc
     3481 taagctgtgc tcaccgctga atttactgag tatgatgctg ggggccgcca cgttaatcag
     3541 tagattgacg agcatgttct cttggggggc gggggcggca gccatagaag gttccgaaga
     3601 tgtggcccag gggcgggcgc aggttacagc aaaaatgagg cgcacttgat atacgattta
     3661 cacacacata gcaatgcctc cgatggcttt ttatcgcctg cggacttagt ggcgcgcgcc
     3721 caggctaaag gggtcaacgc cctggccctc accgaccacg accccctggc gggtttcagc
     3781 caggccgcag ccgccgccca gcaggcggac atcagcctaa tccccggtat tgaattttcc
     3841 acccagtggc tcggccgctc cattcatatt gtcggtttgg ggtttaaccc ggcggcggca
     3901 tccataaccc agggcatcaa gcgccaggca accagccgat tagagcgcgc ccagaccatc
     3961 ggggagaagc tcgccaaagc gggggtgccg gatgcctacg ctggggccca ggccctggcg
     4021 ggcgatgcgg tgctcggccg cccgcatttt gcccagtatc tggtggcctg cggcgccgta
     4081 aaggatgtgg ccagcgcgtt taagcgctac ctgggggcgg gcaaaatggg cgatgtcaaa
     4141 caactctggc ccggcgttga agaagccgta gagtggatta ctgccgccca aggggtagcc
     4201 gtgctggccc acccggacaa atacgacatg acccgcagca aactctgcca gttgctcggc
     4261 cagtttcggg aggcgggggg gcaggcgctg gaggtgatca gcggcaaaca ggtgaacacc
     4321 cttaccgaca agctggcccg cttagccggg cagttttcct tagcggcctc ctgtggcagc
     4381 gactttcatg tgcccaatca accctggcag gaattaggcg catttggcaa actgcccgac
     4441 ttttgccaac ccgtctggca actattgccg ggccatctat cgagttgact atggcgttac
     4501 tgttacagat tcaccccgac aacccccaag agcgactcat taagcaggca gttgaaatca
     4561 ttcggcgcgg cggcttagtg gtgtacccca cggattcagc ctatgcctta ggctgccaca
     4621 taggcgataa gctggctctg gatcgcatcc gcgccatccg ccagttggat aaacaccata
     4681 acttcaccct ggtgtgcaaa gacctttcgg agcttgccac ctatgcgcgg gtaaacaact
     4741 ccgcctttcg cctgctcaaa aaccataccc ccggccccta tacctttatt ttggatgcta
     4801 ccggcgaagt gccccgccgc ctgatgcacc ccaagcgcaa aaccatcggc ctgcgcgtgc
     4861 ccagccaccc cattacccag gcgctgctgg cggaattggg ggagcccatc atgacctcat
     4921 cgctaatcat gccgggggag gagcttcccc tcaccgaccc ctatgacatc cgcgagctgc
     4981 tggatcacca cgtggagttg gtgatcgacg gcggcttttg cggtttggac cccaccacag
     5041 tggttgacct caccgagggg gagccggtat tggtacggca aggccgcggc gacgccagcg
     5101 ccttcaccta attcgccgcc atgcacagcg ctctgcccag tgagcccgcc gccaaacccc
     5161 tggccatagt caagggcgaa gcctggcaga agctacccaa ggatttatac atacccccgg
     5221 aggccctggc ggtcagcctc agccagtttg aggggcccct ggatctactg ctctacctaa
     5281 tccgcaaaca caacctggac attctcgata taccggtgct gagcatcact cagcagtatt
     5341 tggcctacat agaagccata gatgccgccc gggttgagtt ggcggcggac tacctggtga
     5401 tggccgccta tttggtagag attaaatccc gcctgctgct gcctcggccc ccggtggcgg
     5461 aggactgggg ggatgtggaa gacgaccccc gggcggagct ggtggcgaaa ttgctggaat
     5521 atcagcaact gcaggccgtc gccgagcagt tggacgcgtt gccgcgctgg cagcgggata
     5581 ttttgccggt gcaggtatac atacccccca cccaatggcc aacccgctgg gtgcccctga
     5641 ccgcctccga gctggcccag gcctggagcg cggtagcaga gcgccatcgc ttgaatggcc
     5701 agcatgtgat tgaggctgaa gtgttcagca cccgcgcccg tatgggccag ctgctcatca
     5761 ggctgcgcag cgccaacgcc tggcaaccct ttactgggtt attaatcgcc gccgaaggcc
     5821 gcgctggcgt agtggtgacc tttattgctc tgttggagct gatcaaagag ggctatgtgc
     5881 aggcggaaca aaccgcagat gagttgaggt tgtgcgccca tgagcccaac tagctctgaa
     5941 cttaccagcc aaagcagcac tgcagcagac ggcaaaagca gcgcggcttc agccggccca
     6001 aagagcctgg tagaagctgc catctttgcc gccgtcgagc ccatccccac ccgccaatta
     6061 ttaaagctgt ggggcgaggc tgaggatgaa tcccaccgcc agcagctgca ggagatcctg
     6121 agcgcattac aggcggacta ccaaggccgc ggggttgagc tggtgcaggt ggccagcggc
     6181 tggcgctttc aagtgccaaa gccggtggcc cagctacttg agcccctgta tcaagaaaag
     6241 cccaaacggc tttcccgtgt tattctggag accctggcca tcatcgccca ccgccagccc
     6301 atcacgcggg cggagattga agctctgcgg ggtgtggcgg tgagcgctga ggttctgaag
     6361 aatctcatcg agcgccagtg gatctgcagc ctcggcccca aagaaatccc cggccgcccc
     6421 atgcaatacg gcaccaccga tgaattcttg gattatttta atctgcagag ccttgcgcag
     6481 ctgccgagcc tgctgccgcc agccccagca gagccaaacg ccctgagcga catatctgca
     6541 ggcgatacgg cccaactgat taacgacccc aggcctgatg agaatcagcc cgaaccatga
     6601 agaaccccta tgacagatga aaaattgcaa aaagtcctcg ccaccgcagg cttcggctcc
     6661 cgccgtgaaa tggagcgcgt catcgacgcg ggcctggtca ccgtcaacgg tgtcaccgcc
     6721 accctcggca gccgggtaaa agcgggcgat aaactgatta tcgacggcca ccgctaccag
     6781 gtgcccgacc gcaaagaaac ccaagatgaa ttgcgcgtgc tgctctacaa caagccggag
     6841 ggggaaatct gcagccgcaa cgaccctgag ggccgcccca cagtctacga ccgcctaccg
     6901 cccttaagca ctggccgctg gatctccgtg ggccgcttgg acttcaacac cagcgggctc
     6961 ctgctgttta ccaataatgg ggaattggcc aacaaactca tgcacccttc ctcaggcatc
     7021 gaccgggaat atctggtgcg ggtgcagggg gaggtcagca aagaacaact gcaacaactg
     7081 cgcgatggcg tgcaattaga cgacggcctg gccaaattca ccgacatcac tatcggcggc
     7141 ggtaaaggcg ccaacacctg gttttactgc accctgatgg agggtaaaaa ccgcgaagtg
     7201 cgcaggcttt gggaggccca gggctgccgg gtcagccgct taaaacgggt gcgctacggg
     7261 cagattctga tcccctctta tgtgcgcatg ggccagtggg tggagcttgg cttggcggaa
     7321 accaccctct tgtgtgacgc cgccggtatc aaacctccgg cgaagaaaac cctcacccgc
     7381 aatgaatttc tccagcgcga gcgaagagag cagcgcctgc gggcgtcccc tggcaagccg
     7441 gtggcggtta aatcccgcgc ccaccgcagc cgcgctgagc gagaggcctt tatcgctcgc
     7501 gccagtagcg atccccacgc caaacccggc attcaggggc gagcagatac ggcaaaaacc
     7561 aaaacgcgcc cggaagagac caagccggcc aaaggcgggg cggggcgcgc taagcctgcg
     7621 ggaaaaactg ggaaggaaac tgggagcgcc agagtagagg cgggaaaaac cacggcaggc
     7681 cctagaacgg ccagtacaga ggcgagatca acgcaaactg acaagccccg gtcggtaaaa
     7741 gccccccaga gtaaatccgc ctctgcagcc agatctgcga gccctaaacc caatgggcaa
     7801 tctcaatcaa aacctgcggc cccgcgccca agatcgccac ggtctaaaaa agactgagct
     7861 gccaagttac cggcggtgcg agccttcttt aggtttcgct tgccccagct atcggggcga
     7921 gcacgcccgg ggcgaccttc gcagtcgccc cggctaattc cctccagctg ccaaatgccc
     7981 tgcaagatta cttgcgccaa gcccagcaca gactgagttg tgacttttgt gctccggaat
     8041 tgatagttca ccatgcccac aggttagggc acccgtcata ccaacctcct ttaaacccca
     8101 aagctgcagg ccttaataca cattattaaa tgatttaatc cactgctatc ccttagcaca
     8161 tttgtgtaag gcgtgattaa gttggctgat tctctccttg cacgatcggg cacccatcat
     8221 aaaatgtaac cgtttactta aggccgcctg ggcgagtttt tacgccctgg cggatgttga
     8281 gaaacactaa gagggctatg ggcgggcact tataaacccg ccctatctaa ttaaacgcgc
     8341 cactagctgc caaggctgct tgatgagtgc gtacacaaaa caacaatgag gtctttatgg
     8401 ctgaggtaaa acaactggtt ctgggcgctg ctgtggcagc cgccctaggt agcactgcgg
     8461 cgtacgcggg ctttagcgtc agcggcaccc aactgctgga aggcaatggc cgcgcttttg
     8521 ttatgcgcgg tattaaccac gctcacacct ggtacagcac acgtacccct aaagccatgg
     8581 ccgatatagc ggccacgggg gcgaacacgg tgcgggtggt tttaagcgac ggccagcaat
     8641 ggagtaaaac accggccagt gaggtggcga acatcatcaa gctgtgtaaa gacaacaaac
     8701 tgatttgtat gttggaagtc cacgacggca ccggttatgg agagcaaagt gcggcgggca
     8761 ccgtagccaa aatcgcccaa tactgggttg aaatcgcccc ggttttaaaa ggccaagaag
     8821 actatgtact ggttaatatc gccaatgagc cgtttggcag cggtgtggcg gcctcggttt
     8881 ggatcgacgc ccataaagca gccatccaaa ccctgcgcaa tgccggtctc acccatacca
     8941 ttgtggtgga tgcccccgcc tgggggcagg atggcaatga ggccatgctg gccaatgcca
     9001 aaaccgtggc agctgcggat acgcgcaaaa acaccatctt tagcgtacat atgtacgagg
     9061 tctaccaatc gcgcagcaag gtggaaaact accttacaaa attcctcagc agcaataatt
     9121 tacccattat cgtcggtgag tttggggtgg aaaataacgg caaccaggtg gatgtggagt
     9181 ctatattggc cgtcgcccaa caatacaaaa tcggttattt gggctggtcc tggtccggca
     9241 atggcagctg ctgtgtacct ctggatatag tgaaagactt caaccccgca acccccagca
     9301 cctggggaac acggttaatt agcggcacta atggcattaa acaaacatcg gtggtggcga
     9361 gtatatatgg cggtgtgcca tcgagttcct cgagcagctc atcctcatcc agctcatcgt
     9421 ccagtagttc atcttcaagt agcagctctt cctccagcag ttcctcttcg agtagctcag
     9481 gctcaggtcg aacctctagc tcgtccagct caagtagctc atcaagttcc agcggctcct
     9541 caagctcaag cgggggtaat gctgccggca gctttgacag tctggggctt tgggggctgt
     9601 gtttagccgc attggggctg ggccgccgcc gtaaggcagt ggtttaaggc gcgggccaaa
     9661 acagggaggg cggcctgaca attattcgaa aggaagttgt tgccaaaagg caatcctaaa
     9721 aaccgcagcc tcgctgcaag ttcaactgcg gtttttaggt ttaactaagt ttttacgctc
     9781 aatacgcctg gggctggcgg aacatcatta tggccgtcat gaggattatt ttgacatcca
     9841 gcgccaccga ccaattgcgc agataatcca ggtcaaactc aatgcgcgcc tgcattttat
     9901 cgagggtttc ggtttcaccg cgcaggccgt taacctgagc ccagccggtg ataccgggtt
     9961 taaccttatg gcggatcatg taccctttga tttgtttgcg gtacagctca ttgtgggcca
    10021 ccgcgtgggg gcgtgggcct acaatactca tgcgcccctg cagcacatta ataaattgcg
    10081 gcagttcatc cagcgacgtt ttgcgaataa acgcacccac ccgtgtaacc cgtgcgtcat
    10141 tgcgctgggc ttgtttcacc tcactgccat tttcacatac ggtcatggag cggaatttat
    10201 acacagtaat ttcttcgcca tttaaaccgt agcggcgctg tttaaaaata atcggccctg
    10261 gcgatgaata tttcaccgca atcgctacgg caatgagcac aggcattatc agaatcaaaa
    10321 tcaggctggc cagcactata tcgctgatgc gtttcaccaa accattgacg ccagtaaaag
    10381 gggtttcgca taccgaaacc accggcatat tagccacctg gctgatattg ccctgaatta
    10441 aatcagtgac aaataaatcc ggcacaaaat aaatggatgc ggtggtgtcc cgtaaatcgt
    10501 cgagtatttt taaaatacga tcctgggttg ccatggggag ggataggtaa atcaggtcga
    10561 tatggtgttc tttacaaaaa tggctcagct gcgcagttgt gcccaaaaat ggccaaatat
    10621 tgtattgaga tacccgcgca gggtcgcgat cttcaaaaaa accgaccaag tgcgcattgc
    10681 tgtaggggtt gcttttgatg cgtttggcca gctcaatgcc ttgatcattc atgcccacaa
    10741 tcaccgcact gcggctctgc ccctgtagct tgagtagcca gggtgaagcg gcgcgaaaca
    10801 gcaggtggct accgacttgg gcgagcggcc caatccaaaa ccagttggca atagcatcct
    10861 tgtggaacag gtttaatgag cgggtggcat aaccgagaaa aataattaac gatgcaatcg
    10921 taaaccagcg cagggtaata aacgccacgc tgcgcagcag gctttcctgc aggcgtggac
    10981 gccccggaaa ggtcaccgaa aatgccag
//
LOCUS       DAOQGS010000062        10967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2788_length_10967_cov_4.877474, whole genome shotgun sequence.
ACCESSION   DAOQGS010000062 DAOQGS010000000
VERSION     DAOQGS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_10967_cov_4.877474"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKZ52_03765"
     CDS             <1..635
                     /locus_tag="PKZ52_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNG58918.1"
                     /translation="LDKKVVLKSGGYLVIEQTEAMTVIDVNTGAFVGHRNLEETIFKT
                     NLEAAAAIARQLRLRNLGGIIVIDFIDMAEEEHRRQVLRTLEKCLERDRAKSSIHGVS
                     HLGLVQMTRKRTRESLSQILCEPCPACQGRGLLKSAETVCYEMFREILREARMYDNKK
                     FLVLASQVVVDRLLEEESANVADLEAFIGRPVEFQVESLYNQEQYDIVLV"
     gene            639..4679
                     /locus_tag="PKZ52_03770"
     CDS             639..4679
                     /locus_tag="PKZ52_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNG58919.1"
                     /translation="MLLSTLRRLRRLARYFWGSLAVVVIVLAVVVSIGRKLLPMAHEY
                     RADIAAFASRHLGVQVQIGAIEGAWGGFRPRLKIRDLNVQATSGEPIVSAELLETQID
                     LPSLLWDWRLAFRKVQFTGVKARFNQHDDGRWSVNGLALSAPRQSEFSLRDPLDIFLF
                     GRRIQLNNARLDFNFRTGHATQVVLPQILLENDDDFHRLKLGFAVDKDQKALSLVVEG
                     RGDPRDNEHFAAKGYLSLEHFPMEKVVAATALAAWQHLDQGRWSDGARVNLKLWFDGN
                     LAKGFALTGTMQAQDLPLKLPANLRLPERTEANISGRWQADAGWQLVLGQLQLHWPEV
                     SSPPVNISLGGKLGESLLLAMDRFDITAWRQLASRLELHHPVLDGLAPEGVLSNIRLR
                     QASAEEGYFDLEANLTDVGVYSINGSPQIRHVDGYLHTSALGGTLDLASQTAFSMHYP
                     SVYKEPLDYDSAQGQLRWALDSANKQVLVSSSRLSLRGAEGPAEGHLYLALPTEKTPT
                     SEPIMELVVGLKNSQAAYHKKYVPYTVNPKLLGWLDQAIKEGHLIDGGFIYRGSLLKT
                     PITSRSMQLYLNVDNARLAFDPHWPELTDVKARLIMDDSDLRVDVSEGTLAGNKIHDT
                     QVWLSREPGLTLALEGQMQGSSQAALDLLKHSPVATLAEDALRQLDLTGPLQGAIALR
                     VPLAEGGASWQEVAVEVQENHLDLVGLNLALSDVNGSLRYRSEDGLTSPGIMAKLWGE
                     PISAEIKAGEQGNLIEARGPVQVAALNQWLKRPELNYAQGRAEVASRIQLPNFKGGGD
                     RALQLEFTSDLRGVAFNAPAPLGKTAAEALPFKGLMRLDKTSGEDTYHFSIGPQADIS
                     LRRRAGKLDAVGVRLLEGTPRPLQSGHTLVDIAYPQATLTDWQDFTLGYIALLTLANP
                     PAAPSAPAPKTSRKTAHAQHMEVTPEAASETSLPVDLHLELNNLQAGSLALTPVHAQL
                     RQEAANWNLSFNTPSAQGTLTYRANSPSSPWLLQLAALNLPESANRKDDGKCTPYNTS
                     GDYVLPPSSLADINPASLPPAQVHIERITQGGKPIGAVSFNIEPIAQGVKLSQLRGRV
                     YAMQLLGFNRPDAELIWIQKEGKNTTSFDARLRTVNLADVLVAMDEPASITSAAASLN
                     SSLTWEGLPDQGGMASYLGFVQMDIQKGAFIKGVNSGANPLLKLLGFMNFDNLGRRLR
                     FDFADLNSQGMSFDQVSGRIDFNRGQLVLAEPLKVESASTQLKLAGTLDVATQNVNAT
                     LVATLPVSGNLTLAAAMTGALPVAAGVFIAGKIFKKQLDKASSLKYKVRGPWQEPDIA
                     LQTIFDDEAGIEVEAPKKIRPRKISK"
     gene            5006..8782
                     /locus_tag="PKZ52_03775"
     CDS             5006..8782
                     /locus_tag="PKZ52_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNG58920.1"
                     /translation="MGKIFIGVKDLLDMCIRPSRLFLLLSLVISSLVLPRFAYALYVS
                     SATSDHTVYFLFSAPNIIERYDMAAQEELGPIALPGVPTAMAISGAHAFVALHRELYA
                     LDLETEELEYVRGFSEDVTSLVILGENLYATTQSGANTSVLALADHHLIETHADFYNG
                     KQFLGSEKNQAIYFRSVGVSPSDIRKFKVLTNGGLSSSQDSPYHGDYPDASIVYLNAS
                     ESRVYDNSGIAYFAADLTYAGSLGGPVDTLTFIGDNPIVARGTKLQVYSASNAPQGEI
                     TLATKPTVLAAWGNTVFAFTTSPHMKATAVALDQFALPKPGEAVNPKDLAYTPEFIDS
                     DGEDKIYLVDKETLSVFVYSTSQKQYIASWPMLAPPTWASYSAAHKRLYLGFANGEIK
                     YFATDVETPKEQHLITLPGPISGLLSAGNYVFAHDPSGAWGTHYLINAAGSIVSSVEW
                     RNYSAQYVYNPQLGRIYHHRDGTSPNDIEWSDFNTQTGIWGREGDSPYHGDSLQIRLP
                     LRVSADGQLLINGAGQILDASSLVILNSLPTNLKDALWLGDNLITVTESGGAIQFWNR
                     SFVPLKSYAYAGVQNIRLFNLAGKLSLVLQKAGIPDFKLIDLQNLPDTDGDGLDDLSD
                     NCELVVNADQSDFDKDRMGDACDLDDDNDGLSDVDEIKLGLNPLDGTDLSWDNDGDGF
                     SNSVEILLGSDFNQAQSVPKAISHMEEKFDNGPGLGFKLNTGTKPWIWSASAGLEQSG
                     GLMSAPGALGDSVTEFTSVFEAGALSIQAKAEGNYLDLIDLEITVNGQSSRYRLNAGW
                     QKFNIAIPKGLKTIQFKTRSDYSSFLDLSNTLVKIDQFIFDKDTDNDGVPDVADNCPN
                     VYQLSQSDYDGDGFGDQCDPDPYVVTPRTDGDGDSILDVFDNCPSVANPDQSDVDGDK
                     LGDACDAIDNRPKDIDNDGIIDQFDNCPTVANPTQGNLDGDSRGDACDEDADGDGVLG
                     SIERLYPFMSDSNPQDTQLDSDQDGVDNGFEIAHGTDPGQFNQFPINNLLDYFPLQEA
                     VYTYASEFNFFEINYTKLDSFNEIVAHLPGGSVERYSASAQGVIMTSASEYVGVLGQS
                     LGGKYVGNVVMPKALKLGESVVFAAKRYTLDSQSGEPDNFSVHIRLLDMGYQPVNGRS
                     LPSITLERTEYSNGKLISARKNFYIKGIGLGEFNATPLVSYEAKKIEVAPPVIEPKPD
                     PKPAPQPEPKPTTPAQPVQDKVAGSLDAWIVLGLMVVGLRRKKLVIGNFSKSYS"
     gene            10771..>10967
                     /locus_tag="PKZ52_03780"
     CDS             10771..>10967
                     /locus_tag="PKZ52_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58921.1"
                     /translation="MLTGQIKLTSQTVGQLKRLYSNLGYGLAFICHTYGGFMFYRYSK
                     ILSAVFKAVAAVGLLGLSLPG"
BASE COUNT         2680 a         2786 c         2812 g         2689 t
ORIGIN      
        1 ccctggataa aaaagtggtg ctcaagtcgg gcggctattt ggtgatcgaa cagactgaag
       61 ccatgacggt gatcgacgtt aacaccggcg cttttgtggg ccaccgcaat ttagaagaga
      121 ccatttttaa aaccaattta gaggccgccg ccgccattgc ccgccagctg cgcctacgca
      181 atttgggcgg cattattgtc atcgacttta tcgacatggc cgaggaggag caccgccgcc
      241 aagtgctgcg caccctggag aaatgcctgg agcgggaccg cgccaaaagc tccatccacg
      301 gcgtctctca cctgggcctg gtgcagatga cccgcaagcg cacccgagag tccttaagcc
      361 aaatcctctg cgaaccctgc cccgcctgcc agggccgggg gctgttgaag tcggcggaga
      421 ccgtctgcta tgaaatgttc cgcgagatcc tgcgggaggc gcgcatgtac gacaacaaaa
      481 agttcctcgt gctggcctcc caggtggtgg tggatcgatt gttggaggag gaatccgcca
      541 atgtggcgga cttggaggcg tttatcgggc ggccggtgga attccaagtg gagagcctct
      601 acaaccaaga acaatacgac atagtgttgg tgtaaccggt gctgctctcc acccttcgcc
      661 gcctgcgccg cctggcccgc tacttttggg ggagcctggc ggtggtggtg attgtgctgg
      721 cggtggtggt aagcatcggc cgtaaactct tgcccatggc ccatgaatac cgggccgaca
      781 tagccgcttt tgcctcccgc cacctggggg tgcaggtgca gattggcgct attgaagggg
      841 cctggggagg ctttcggccg cgcctgaaaa tacgcgacct caatgtacag gccaccagtg
      901 gtgagcccat cgtcagcgct gaacttttag aaacccaaat cgacctgcca agcctattgt
      961 gggattggcg cctggccttt cgcaaagtgc agttcaccgg tgttaaagcg cgttttaacc
     1021 agcacgatga cggccgctgg agcgttaacg gcctggcgct gagcgccccg cgccaaagtg
     1081 aatttagcct gcgggacccg ctggacatct tcttattcgg caggcgtatt cagctgaaca
     1141 acgcccgcct agattttaac tttcgcactg gccacgctac tcaagtggtg ctgccgcaaa
     1201 ttctgctgga aaacgatgac gactttcacc gcttgaagct tggctttgcg gtggataaag
     1261 accaaaaggc cttaagcctg gtggtggagg ggcggggcga cccgcgggac aacgagcact
     1321 ttgccgctaa gggctattta agcctggagc acttccccat ggaaaaggta gtggccgcca
     1381 ccgccctggc cgcctggcag catttggatc agggccgctg gagcgacggc gcacgggtga
     1441 acctaaaact ctggtttgac ggcaacctgg ccaagggctt tgcacttacc ggcaccatgc
     1501 aggcccagga tttacccctc aaattgcccg ccaacctgcg cctgcccgag cgcaccgagg
     1561 ccaatatctc cggccgctgg caggcggatg cgggctggca gctggtgctg gggcagctgc
     1621 aactgcactg gccggaagtg agttcaccgc cagtgaatat aagcctgggg ggcaagctgg
     1681 gggaatccct gttgctggcc atggaccgct ttgacatcac cgcctggcgg caattggcca
     1741 gccgcttgga gctgcaccac ccagtgctgg atggcctggc cccggaaggg gtgctcagca
     1801 atatccgcct gcgccaggcg agtgccgagg agggttactt cgacctggag gccaacctca
     1861 ccgatgtggg tgtatacagc atcaacggca gcccgcagat tcgccatgtg gacggttatc
     1921 tgcacacctc ggccctgggc ggcaccttgg acttagcctc gcaaaccgcc ttttccatgc
     1981 attacccttc cgtctacaag gagcctttag actacgactc tgcccagggc cagctgcgct
     2041 gggctttgga tagcgccaat aaacaggtgt tggtgtcctc ctcccgcctg agcctgcgcg
     2101 gcgccgaagg ccctgctgag ggccatttat acctggcgct gccgacagaa aagaccccca
     2161 cctctgaacc cattatggaa ttggtggtgg gccttaaaaa ttcccaggcc gcctaccaca
     2221 agaaatacgt gccctatacg gtcaacccca agctgcttgg ctggttggat caggctatta
     2281 aagaaggcca cttgattgac ggcggcttca tctaccgggg ctcactgctc aaaaccccca
     2341 tcacctcccg cagtatgcag ctgtatttaa acgtggataa cgcccgcctg gccttcgacc
     2401 cccactggcc ggagctgacc gatgtgaaag cccgcctgat catggacgac tccgacctgc
     2461 gggtggatgt gagtgaaggc accctggcgg gcaataaaat ccacgacacc caagtgtggc
     2521 tcagccgcga gccgggcctc accctcgcct tggaagggca gatgcagggc agcagccaag
     2581 ccgccctcga cctgttaaaa cacagcccgg tggctaccct cgccgaagac gccctgcggc
     2641 agttggacct caccggcccg ctgcagggcg ccatcgccct gcgggtgccc ctggccgaag
     2701 gcggtgccag ttggcaggag gtagccgttg aggtgcagga gaaccacctg gacctggtcg
     2761 gtttaaacct ggcccttagc gatgttaacg gcagcctgcg ttaccgcagc gaagacggcc
     2821 tcaccagccc aggcatcatg gccaaactgt ggggtgaacc tatcagcgcc gagattaaag
     2881 cgggcgagca gggcaatctg atcgaagcgc gcggcccggt gcaggtggct gccctcaacc
     2941 agtggcttaa gcgcccggaa ctcaactacg cccagggccg cgccgaggtg gcaagccgca
     3001 ttcaactgcc caactttaaa ggcggcggtg atagagccct gcagctggaa ttcacctccg
     3061 acctgcgcgg tgtggcgttt aatgcccccg cacctctggg caaaaccgcc gccgaagcgc
     3121 tgccgtttaa agggcttatg cggctggata aaaccagtgg cgaggacacc taccacttca
     3181 gcattggccc ccaggccgac ataagcctgc gccgccgtgc cggtaagctc gacgccgtgg
     3241 gggtgcgcct gctcgagggc acccctcgcc ccctgcaaag cggccacaca ctggtggata
     3301 tagcctaccc tcaagctacc ttaaccgact ggcaggactt taccctgggc tacatagcgc
     3361 tattaaccct cgctaacccc ccagcagcgc ccagcgcccc agcccccaaa accagccgca
     3421 aaaccgccca cgcccagcac atggaggtaa ccccagaggc cgccagcgag accagcctgc
     3481 cggtggatct tcacctagag cttaacaacc tgcaggcggg cagcctcgcc ctcacccccg
     3541 tgcacgccca gctgcgccag gaggcggcca attggaacct aagctttaat accccgagcg
     3601 cccagggaac cctcacctac cgcgccaata gccccagctc accctggttg ctgcaactgg
     3661 ccgccctcaa tttaccggag agtgccaacc gcaaagacga cggcaaatgc accccctata
     3721 acacctccgg cgactatgta ctgccgccct catccttagc cgatataaac cctgcgagcc
     3781 tgccgcccgc ccaagtgcat atcgagcgca tcacccaggg cggcaaaccc ataggcgcgg
     3841 tgagttttaa tatcgagccg attgcccagg gcgtgaaact cagccaattg cgcggccgcg
     3901 tgtacgccat gcaattgctc ggctttaacc gcccggatgc agaactcatc tggattcaaa
     3961 aagagggcaa aaacaccacc agcttcgacg cccgtttgcg cactgttaac ctcgccgatg
     4021 tattagtggc catggacgaa ccggcctcca ttaccagcgc tgccgcgagc cttaattcca
     4081 gcctcacctg ggagggcctg cccgatcaag gcggtatggc cagttactta ggttttgtgc
     4141 aaatggatat tcaaaaaggc gcgtttataa aaggcgttaa cagcggcgct aatccattat
     4201 taaaattatt aggttttatg aatttcgata atttaggccg cagattacgt tttgattttg
     4261 ccgatttaaa ttcccagggc atgtccttcg atcaggtcag cggccgcatc gattttaatc
     4321 gcggccaatt agttttagcc gaacccttaa aagtggaatc cgcctccacc caattaaaat
     4381 tagcgggcac tctggatgta gccacccaaa acgtcaacgc cacattggtt gccaccttac
     4441 ccgtctccgg caacctaacc ctcgctgccg ccatgaccgg tgcactacct gtggcggcgg
     4501 gggtatttat tgccggcaaa atatttaaaa aacaattaga taaagcctca agccttaaat
     4561 acaaagtgcg cggcccctgg caggagccgg atattgcctt gcagactatt tttgatgatg
     4621 aggcggggat tgaagtggag gcgcctaaga aaattcggcc gcgtaaaatt tcaaaataga
     4681 tataagcaat cattgcattt ggcgataggc caaccgagca gaagtcatgg ggctgcaaaa
     4741 ttgccttatc agtcaaatcg atgtcttcaa taccccctcg taatttttca gttcctatgt
     4801 cttggggctg atgcgctaag cacatcaaat acagtgaatt taatttactt gccggtggca
     4861 atagcctgca tttttggcta ccatgcggcg cccttaaagg attctaaggc aatcattagc
     4921 gaatccttgt aagtggcgtg ccgatgctga tgtcggcaaa tttactccca cattacaatc
     4981 gattctcaat agcattggag gccacatggg taaaattttc atcggggtta aggacctgct
     5041 tgacatgtgc atacgcccgt cgcgcttgtt tttactgctc agcttggtaa tttcgtctct
     5101 cgttctgcca cgttttgcgt acgcactgta cgtaagctcg gcaactagtg atcacaccgt
     5161 atactttctt ttctctgcgc cgaatatcat cgagcgttac gatatggctg cgcaggagga
     5221 attaggacct atcgctctgc ctggtgtgcc aacagcaatg gcaatttccg gcgcgcacgc
     5281 gtttgtcgcc ctgcatcggg aactctacgc gctggatctt gagacggaag aattagagta
     5341 tgtgcgcggc ttctccgaag acgtcacctc gctcgttatt ttgggtgaga acctgtatgc
     5401 gaccacccag tcgggtgcta ataccagtgt ccttgccctt gctgatcatc atttaattga
     5461 aactcatgcg gatttttaca acggcaaaca atttttgggt tctgagaaaa accaagcgat
     5521 ttattttcgc agcgtaggcg tatcgccatc ggatattcga aaatttaaag ttttaaccaa
     5581 tggtggcctc tcgtcctctc aggatagtcc gtaccacggc gattaccccg atgccagcat
     5641 tgtctattta aatgcaagtg aatccagagt gtacgataac tcaggaatcg catatttcgc
     5701 cgcagactta acctacgcgg gcagcctcgg cggacctgtt gacaccctga catttattgg
     5761 tgataacccg attgtggcgc gcggcaccaa gttacaagtg tattcagcca gcaacgcccc
     5821 gcaaggggaa attacgcttg ccactaaacc cactgtactc gcagcgtggg gtaatacggt
     5881 atttgcattt actacctctc cccatatgaa agctacagct gtggctcttg accaattcgc
     5941 gttacctaaa ccgggtgaag ctgtaaatcc aaaagatttg gcatacactc cggagtttat
     6001 tgacagtgat ggtgaggata aaatttattt agtcgataaa gaaacactca gcgtatttgt
     6061 ttacagtaca tctcagaaac aatatatagc tagttggcca atgcttgcgc cgcctacttg
     6121 ggccagttat tccgccgctc ataagcgctt atatttgggg tttgcaaatg gcgaaattaa
     6181 gtatttcgca actgatgttg aaacgcccaa agagcaacat ctcattacat tacctgggcc
     6241 aatctctggc cttttatccg caggtaatta tgtgtttgcc cacgatccat cgggtgcctg
     6301 gggaacgcat tatttaataa atgccgcagg atcaattgtg agctctgttg agtggcgtaa
     6361 ttatagtgct caatacgtgt ataacccgca attgggccga atttaccatc accgagatgg
     6421 gacatcgccc aatgatattg agtggtctga cttcaatacc caaactggta tctggggccg
     6481 agagggcgac tctccctatc atggcgattc tttgcaaatt cgcttgcctt tacgtgtttc
     6541 agccgacggc caactattaa ttaatggcgc agggcaaatt ctagatgctt ccagtctggt
     6601 gattttaaat tcacttccta ctaacctgaa ggatgcgttg tggctgggcg ataaccttat
     6661 aacggtcact gaatcaggcg gggccatcca attctggaat agaagttttg tacccttaaa
     6721 atcctacgcc tacgcgggtg tgcaaaatat tcgtttgttt aatttggcgg gcaaactcag
     6781 cctagtactg caaaaagctg gcattccaga ctttaaatta atcgatttac aaaatctgcc
     6841 agacaccgat ggcgatggcc tggatgactt aagcgataat tgtgagttag tagtcaatgc
     6901 cgatcaaagt gattttgata aagaccgaat gggcgatgca tgtgatttgg acgacgataa
     6961 tgacggcctt agcgatgttg acgaaattaa actcggcctg aatccgctcg atggaaccga
     7021 tctgtcatgg gataatgacg gtgacggttt ttctaatagt gttgagattc ttttaggtag
     7081 cgattttaac caggctcaat cggtgcctaa agcaattagc catatggaag aaaaattcga
     7141 taatggcccc ggccttggtt ttaaactgaa cactggtacc aagccctgga tctggagtgc
     7201 aagtgctggc ctggagcagt caggtgggct tatgtccgct cccggagcac taggggattc
     7261 agtgactgaa tttaccagcg tatttgaagc gggtgcttta agtattcaag cgaaagcaga
     7321 gggtaattat cttgatttaa ttgaccttga aattaccgta aatggccaga gcagccggta
     7381 ccgtttaaac gctggctggc aaaaattcaa tattgccatc cctaaggggc tcaaaacgat
     7441 ccaatttaaa acgcgatctg attattcttc atttttagat ctatcaaaca ctctggttaa
     7501 aatcgatcag tttattttcg ataaagacac tgacaatgac ggtgtgccgg atgttgccga
     7561 taattgccca aatgtttatc agctgtcgca atccgactat gacggtgacg gcttcggtga
     7621 tcagtgcgat ccagatccct acgtggttac gccgagaacc gacggggatg gcgattcaat
     7681 tttggatgta tttgataact gcccgagcgt tgccaatccc gatcagtccg atgttgatgg
     7741 cgataaactt ggcgatgcct gtgacgccat cgataatcgc ccaaaggaca tcgacaatga
     7801 cggcataatc gaccaattcg ataattgccc aactgtcgcc aaccccactc aagggaattt
     7861 agacggcgat agcaggggcg atgcgtgcga tgaagatgcc gatggtgacg gcgtgttagg
     7921 cagtattgag cggttgtacc cgtttatgag tgatagcaat cctcaagata cacaacttga
     7981 tagcgaccaa gatggtgttg acaacggttt tgaaatcgct catggcactg atcctgggca
     8041 gttcaatcaa ttcccaatca ataacctttt agactatttc ccgttgcagg aagctgtata
     8101 tacctacgcc agtgaattta acttctttga aattaactac acaaagttgg atagttttaa
     8161 cgaaatagtc gcacacctgc ccggtggttc agttgagcgt tatagtgctt ctgctcaagg
     8221 ggtaataatg accagtgctt ctgaatatgt cggtgtttta gggcagtctc taggtggaaa
     8281 atacgttggt aacgtcgtga tgcccaaggc attaaaatta ggggaatcgg ttgtttttgc
     8341 agctaagcga tataccctcg attcgcaatc gggagagcct gataatttta gcgttcatat
     8401 ccgtttgctt gacatgggtt atcaacctgt aaatggccga agtttaccga gcattacact
     8461 ggagcggact gagtattcaa atggtaaatt gataagtgcc cgaaaaaact tttatataaa
     8521 aggcattggt ctgggtgaat ttaacgctac accgctcgtc tcctatgaag ctaagaaaat
     8581 tgaggtggca cctccggtaa ttgaaccaaa accggatcca aaacctgcac cccaaccgga
     8641 gccaaaaccc acaactccag cacagcctgt gcaagataag gtcgccggaa gtttggatgc
     8701 atggatcgta ttggggctga tggtggtagg tttgcgacga aaaaaattag tcattggaaa
     8761 tttttctaaa tcgtatagtt gaggtatcag ctatacacac caccgataaa ggggcgcagt
     8821 tgcgcccttt ttttcttgat gatcaccagg tattaatcgc agaagatcag cgttcttacg
     8881 gggcaagtga ttgactaaaa aggaaaataa tactgccttg atgacgaggt gagggcgcga
     8941 aaagtgagtt tctagtggtg acctaagtgg ttgctaaaaa ttgcaggttg ttctcgggaa
     9001 gctgcttcag ctcctctcta tatcgctatt aaaaatacct gttgaataat ttagatgggt
     9061 acgctctaga attcgagggg ctccatctct taaattctta ggctgaggga atagaatagg
     9121 tagctatata agacctgttg tccccattcg tcttgtgaca aataagccgc ctaagtgccg
     9181 tctgtattca aaatgcaagg tatgcctctc tctgggcatt aatgagagta taccttgcat
     9241 tcgctaggga attacttgtg attcaataca gcacattgag gaacgctggg gtcaaaaaaa
     9301 catttttgtg ggaatccccc ggggagctgt atacagtata gcaggcaggt cggcgatctt
     9361 aacgctttca gagggctaaa aagctgacca aaatttgcgt ttgcctgggg tgtctcattt
     9421 ggtaatttac ggtctaattc tgcggctgaa gaaaaacatg ataacaataa aaaccctaca
     9481 aggctgattg agcgagcgaa atgttgcact ttcatagtga ttcctatttt gtgttgagtg
     9541 gttagtccaa gttgggctta ggtgagcaaa agattagctt gtatcgccta agctcgagta
     9601 ttgtgcggct atttaataat gttgtctagc atgttttcaa tctaatttat gtggtaatta
     9661 ggtcgaatag agcgcggcga aacccaacct ctaatgaggg agaaagttgt tttgcgttgc
     9721 tataagttta acgacgcccc atattattct ggttgtgaaa tgcattgtgt tgagttttgc
     9781 tgccttctgt tcaacctact tactaggttt aactctggcg gtgaaccaaa cataatcttg
     9841 ttaatggctg agtgcttaac gatgaagtct ttaagattag tctcttgttt cgtggttatt
     9901 gcatattttg tggtctacat gttcttaacg gcgtttggcc gatatgagga taacctaaca
     9961 acactgtcaa aaatttatca tgattgtttg tgtatttctg atttggaact ttggcaaccc
    10021 aaatacgtat tgtttacccg ctacaatggg cgaatatatg caaatacttt gggtctgatt
    10081 tattcgccat taatattttt agatcagaaa tgttggcaca aggataaaga gtttaatctt
    10141 gattaacttt gcgtgggggt ttatgccttg taacggatcg tcttgtgtag ccgtagatgc
    10201 cagcgtgttc atcaaaagag gatggccaca aaaaatcaaa cgcgtaaccc gcattaaatt
    10261 gtaattttgt agggcaatga gatggactcc ccctttctac tttcgctaaa actgtgtttt
    10321 tagtgtggca ctttgatgcc gggttgttag agagggggag tccatctcat tgccctactg
    10381 aacgccaatt aatacgggtt acgcgttgct ggcattcaac gctaaatcaa cccagccgta
    10441 tcattgagcc aacccgcata accctgtttg attacaccga aggcggcaag cttaaatctc
    10501 aaatcgtaaa acctcgccca gctggagtgg taaattgctt aaccccctta tcctgagcga
    10561 gttacattta gcaggtaaaa aggttccgag agcagcctag ccctgccgct aggctgggag
    10621 cttcgcccag agtgttgatt tttgccatag taggtaattg attcaacgtg acagaccttt
    10681 cgttcaaata ttacctttcc ggcaggtaat ttattcgata gccaatattt tcatattgca
    10741 actggcaaaa ggttgagttg actacaccag ttgctaactg ggcagataaa attaaccagt
    10801 caaactgtcg gccagttaaa acgtttatac agcaacttag gttatggttt agccttcatt
    10861 tgtcacactt atgggggctt tatgttttat cgctatagca agatcctgtc tgcggtattt
    10921 aaggcagttg cggcagtggg gctgttggga ttgtcgctgc cggggca
//
LOCUS       DAOQGS010000063        10946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2795_length_10946_cov_4.445322, whole genome shotgun sequence.
ACCESSION   DAOQGS010000063 DAOQGS010000000
VERSION     DAOQGS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_10946_cov_4.445322"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKZ52_03785"
     CDS             complement(<1..468)
                     /locus_tag="PKZ52_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNG58922.1"
                     /translation="MDDYLLNAHIDTLAIRAGIARTAEGEHSEPIFLTSSYVFASAEE
                     AAARFDGTTPGNVYSRYTNPSVRTFEERLAALEGGEAAVATASGMAAILSTCMALLKS
                     GDHVVCSRSVFGTTSVLFTKYLAKFGIATDFVSLTDLTAWQQAIKPNTRLLFLE"
     gene            complement(587..2101)
                     /gene="purF"
                     /locus_tag="PKZ52_03790"
     CDS             complement(587..2101)
                     /gene="purF"
                     /locus_tag="PKZ52_03790"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNG58923.1"
                     /translation="MCGIVAMVGKNNVNLALYDALTMLQHRGQDAAGIMTCHNGRLAQ
                     QKSVGLVQDVFHSRHMQRLVGNMGIGHVRYPTAGSSGPALAQPFYVNSPYGIALAHNG
                     NLTNTEQLSNDLFLSDLRHVNTDSDSEVLLNVFAHELHELGKLRPTYEDIFKAVTGVH
                     KRIRGGYAVVAMIANYGVLAFRDPNGIRPLVFGKRTTDLGDEYMVASESVALDVLGFK
                     LIRDVAPGEAIFITPQGDLFTRQCADNPRLSPCIFEHVYFARPDSIMDGVSVYKARLR
                     QGEKLADKILRLRPNHDIDVVIPIPDSSRVAGQALAQKLGVKFREGLVKNRYIGRTFI
                     MPGQQQRKKSVRQKLNPIDLEFRGKNVLLVDDSIVRGTTCQQIIQMARDAGAAKVYFA
                     SAAPAVKFPNVYGIDMPSAKELIAHDKSEEEVATAIGADWLIYQDINDLIAASAEGNG
                     SIPRFDCSVFDGEYITGDVSQAYLNKLAADRNDAAKTGRDLGQGQSAIVGMDNS"
     gene            complement(2131..2628)
                     /locus_tag="PKZ52_03795"
     CDS             complement(2131..2628)
                     /locus_tag="PKZ52_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNG58924.1"
                     /translation="MIWVDWVILGIFGLSSLIGLVRGLVKEAMSLVVWALALFIATHF
                     KAQAAVLLERYIQTPSLREMAAFGGLFIATLFIGALVNFVLGQIVKMTGLGGTDRVLG
                     ALFGAGRGFLVVMAIIIFLPPLIPIDKDNWWHQSRLIPEFQAFEDSARTIFSQMAEAY
                     HRLTS"
     gene            complement(3138..8024)
                     /locus_tag="PKZ52_03800"
     CDS             complement(3138..8024)
                     /locus_tag="PKZ52_03800"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNG58925.1"
                     /translation="MRSTCFNMRSGLIASLLAMHSLAFGLPVVSDYDAVPPLLAESAK
                     PMVMLAVSKDHQLFYKAFTDFDDLDADGIVDTTYKNSFEYAGYFDSYKCYQYDTTLKY
                     FVPISITADKYCAGTNLWSGNFLNWATMTRVDEVRKVLYGGLRAIDTATDTVLERSYL
                     ANDAHSFAKYYEGDDLEKLTPFTLAASCGTAPASKAAPGYATYKACRLARGITLCNTT
                     RYSGVLLSQAVTSPPLMRAVSGNYSLWAANERMQCLFTGESTAGGGNGNDPDATGIFA
                     AATNPAASAVVPQRDGVKVGDYSVKVQVCVSEALRGQEDCKSYPSGNLKPVGILQQFG
                     DDGSALFGLMTGTYKKNKSGGVLRKNVSTFSDELNVATDGTFKAAPATGGIVNSLNQL
                     RLLNYNFNSGSEGTYNSSDNCNWGKNSFSNGECTNWGNPFAEIMLECYRYMAGKTPDS
                     AFDADDTSYFAGLTRQTTWSNPQNATTACANLNVIGFNASTVSYDGDELGKVTDLATA
                     KTAQQLTKTVGDGENITGKYFFVGENGGSGSSDNNQICTAKLVSDLGLAEGTCPDAPR
                     LDGSYRLAGLAYQAHIADLRPDIVDKQSVSTYGVTLSPALPKVTVVNPSNNKVVSILP
                     ACRNNSVSGNCAIVDFKIVSQSVTATEARGTFYVNWEDSEQGGDYDQDMSGVISYVLT
                     STSLTVTSDVFGESTPNAMGFGFVVSGTTEDGFHALSGINSYVGYECTGSGCTGSQAA
                     ASKTFTLGATSVAGSLVSLLQQPLFYAAKWGGFRDSNASNTPDLVTEWDRKNNSTGNT
                     GADGIPDNYFFAIDPKQLKNNLTSILISILERTSSGTSAALVSNTGAGEGALFQALYN
                     PRVTDASGATNVNWVGSLNAYFIDRWGNMRDDNAAPYGTLTADDSILDIYYDPYAKRT
                     YFQRYQITNTGARGSPVGPADDILKLKPIWSANSNLASLTDLTTQRSYSAVASSGRYI
                     FTGIDRTGDGQIGWDAWVNEVEPFVPATFPNGATDNQYRLLGLTSANKADAPNLVNFI
                     RGQEGLPGFRNRTVDYANTGQPKPWLLGDIVNSSPVSVGRPNSSYDVSFGDDTYLEYK
                     LKYEKRRQVVYVGANDGMLHAFNAGFYDPNTLTYKLSSGKGEVEHPLGAELWAYVPYN
                     LLPHLQWLAKADYPHVYYMDGPVKSYDVNIFTPDATHPGGWGTIIVAGMRFGGGDFTL
                     DPNSDPDASSADDITLRSGYVIMDVTDPEQPPVLIGELANYTLGFATPDVTVMKMRAP
                     NSGDGSYSSPAINRWFLVLGSGPSGYDATTKSKALNTAETSGNASLVIYDLQAKSIHS
                     SLYGVQTAAFVGGITAADWNQDYVDDDLYFGTVSGTPAAPSGKLFRVVPTVSGSSVTF
                     ASSLVMNTNQPVSAAPTTLTDHLNNFWVYFGTGRFFVVDDNNSSSQQSYYGIKEPKAA
                     GVMTNAAVTTSSLINTTDIQVFADGAVRSKSTGSSPVTLSNGGSAGAFYDVVDEVSKV
                     SGWYFNFSRARSRNTNKSVVADQSLIFSEYQPSGLKCQPEGQGYLNAPHLFAGIPGYF
                     APIGKDSSVQFNGKDLVNLSASLGLGAPSAPQIHQNSNGVRNAVVQTSTGQITWQTIT
                     AGAVQGKRETWREILINW"
     gene            complement(8140..8769)
                     /locus_tag="PKZ52_03805"
     CDS             complement(8140..8769)
                     /locus_tag="PKZ52_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58926.1"
                     /translation="MTLQPKRLTTIGSQRGAALVVSLVLLAVVTGMQNSTSELKMATA
                     AQDHATAFAAAEAALTQVEQQLAMSPPDREKLLSTCTGTGCFNKTCNDGLCFQGTYIN
                     VDSEFDCKVSDPTGKIAVIDFWSDATLNIWKTASRYKKIKIERLDADVKYITEFLCYV
                     KRDATTPFSGAAGEKNNGAPLFRITAFAEGNGLRGRVMLQSTYKVLAGH"
     gene            complement(8831..9985)
                     /locus_tag="PKZ52_03810"
     CDS             complement(8831..9985)
                     /locus_tag="PKZ52_03810"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNG58927.1"
                     /translation="MDTRINYLPKHSSFSHPASAANAQGFSIVELLIAVTIGLIILSG
                     VINVVVKSKTNKMQQDEVAFIQDNARFVVETLTSEIRMAGYMGCASPTTSLIANSIDG
                     NFFNFVGTGGIEGYDGQTSVAGFPSPLNTLAKVGSDAFIVRRGDSDELGVESHVAASA
                     TISLYKQHSFPPGTPLMIADANCRYVGLFQVSSPNANALPANKIVHNTGAVTSNCTKI
                     IRGSFVCTPSCTAVSCNGYGTTTGSYSPGSKVMRFISRAYFIGPSTVMADMPALKRQS
                     LVMDSTLTTRSEEIAQGVEDMQLLYGVDTNADGDVNQYRTAAQMDVDGSGAIDGKDWD
                     KVKSVRISLVFRSQNPVAPSNQTQVINGVTYNDRYLRQAVNSTVVIRNRM"
     gene            complement(9990..10472)
                     /gene="pilV"
                     /locus_tag="PKZ52_03815"
     CDS             complement(9990..10472)
                     /gene="pilV"
                     /locus_tag="PKZ52_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNG58928.1"
                     /translation="MRTELKPKGNTRQHGSSLLEVMVALVVLGVGLLGVLAMQARSSQ
                     YGQSTYYYAQAVFLANDIADAMRTTPAVAGGYNILFDDPIPNAKTCESTTISCSAGEL
                     RDWNLNKWLTNVARFLPQGKASIQAVGDNTVITVRFNDARAQVDGSEKFFEYVLVTDV
                     "
BASE COUNT         3023 a         2766 c         2527 g         2630 t
ORIGIN      
        1 ttccaaaaat aacaggcgtg tattgggttt aatcgcctgc tgccaggccg ttaaatccgt
       61 aaggctgaca aaatccgtag cgataccaaa tttggctaaa tatttggtaa acaatactga
      121 ggtagtgcca aatacactgc gcgagcacac cacgtggtcg ccggatttta atagcgccat
      181 acaggtactt aaaatagctg ccatgcccga ggcagtcgcc accgccgctt caccgccttc
      241 taatgccgcc aagcgttctt caaatgtgcg cacactgggg ttggtatagc gcgaatagac
      301 attgccaggt gtagtaccgt caaaacgcgc agcggcttct tctgccgagg cgaacacata
      361 actggaggtt aaaaaaatgg gctccgagtg ttcgccctct gcggtgcgag caatgcctgc
      421 gcgaatcgcg agggtgtcga tatgggcgtt gagcaaataa tcatccattg gtttacctac
      481 taataattaa ggcgggaaaa atgaatatgc ttagcaaccg ctaattaatg agtattttcg
      541 acactattca ctaccagcag cgctttaaca gaccaaccaa agccggttaa gaattatcca
      601 tccccacaat ggcgctttgc ccctgcccca aatcgcggcc ggttttagcc gcatcgttgc
      661 gatcagccgc taacttattt aaataggcct ggctgacatc accggtgatg tattcaccgt
      721 caaataccga gcaatcaaag cgcggaatcg agccattgcc ctcggcggaa gcggcgatta
      781 aatcgttaat atcctggtaa attaaccagt cggcacctat agcggtggcg acctcttctt
      841 cacttttatc gtgggcgatt aattccttag cgctgggcat atcaatgccg tacacattgg
      901 gaaatttaac cgctggcgct gccgaggcga aatacacttt ggccgcaccg gcatcccgcg
      961 ccatttgaat aatttgctgg caggtggtgc cgcgtacgat ggaatcatcc accagcaaca
     1021 cgtttttacc gcgaaattct aaatcaatag ggttgagttt ttgccgtaca gattttttgc
     1081 gctgttgttg gcctggcata ataaaggtgc ggcctatgta gcggtttttc accaagcctt
     1141 cgcggaattt aacaccgagt ttttgcgcta aggcctggcc tgccacgcgg gagctatcgg
     1201 gaatgggaat taccacatct atatcgtggt tggggcgcag gcgcagaatt ttatcggcca
     1261 gtttttcccc ctgccgcaag cgcgctttat aaaccgatac accatccata atggaatcgg
     1321 ggcgggcaaa atacacatgc tcaaaaatgc agggcgataa acgcgggttg tctgcacatt
     1381 ggcgagtgaa taaatcacct tggggcgtaa taaaaatggc ctcgccgggc gccacatcgc
     1441 ggattaattt aaaacctaat acatccagcg caacgctctc ggaggccacc atatattcat
     1501 cgcctaaatc cgtggtgcgt ttgccaaata ccaatgggcg aataccgttg gggtcgcgaa
     1561 aggctaatac gccgtaattg gcgatcatgg ccaccaccgc gtagccgccg cgaatgcgtt
     1621 tgtgcacgcc tgtgacggct ttgaaaatat cttcgtaggt ggggcgcaat ttacccaatt
     1681 catgtaactc atgggcaaat acattcagca atacttccga atctgaatcc gtattaacgt
     1741 ggcgcaaatc cgataaaaat aaatcgttgc tcaactgctc ggtattagtg agattgccgt
     1801 tgtgggccaa ggcaatgccg tagggggaat taacgtaaaa aggctgcgcc aacgccgggc
     1861 ctgagctgcc cgctgtgggg tagcgcacat ggccgatgcc catattgccc acgaggcgct
     1921 gcatgtggcg gctgtggaaa acatcctgca ccaagcccac ggatttttgc tgggccaacc
     1981 gcccgttatg gcaggtcatg atgccggcgg cgtcttggcc gcggtgctgc aacatggtga
     2041 gtgcatcgta caatgccaaa ttaacattgt ttttgccgac cattgcgact atgccacaca
     2101 taaaataacc tcaatagaac tatctggaaa ttacgacgta agacgatgat aggcttctgc
     2161 catttggctg aagatagtgc gagcactgtc ctcaaatgct tgaaattcgg gtatgagccg
     2221 cgattgatgc caccaattat ctttgtcgat gggaattaat ggagggagaa aaataataat
     2281 ggccatcacc actaaaaatc cgcgcccggc accaaacagc gcacctaaca ctcgatcagt
     2341 gccacctagg cccgtcatct tcacaatttg gccgagcaca aagttcacca gggcgccgat
     2401 aaacagcgtg gcaataaaca acccgccaaa agcggccatc tcgcgcaggg agggggtttg
     2461 gatatagcgc tctaatagaa cggcagcctg ggccttaaag tgggtggcaa taaaaagcgc
     2521 taaggcccac accactaggg acatggcttc tttaaccagg ccgcgcacca ggccgatcag
     2581 gcttgataaa ccaaagatgc ctagaattac ccaatcgacc caaatcatcg tgccgttgcc
     2641 acccctcgtt gaaagggggc gattctacca gagggggagc caaaggcgaa ccgatttaca
     2701 ttcaagggcc cctatgcctg gcaagccaag taagagcagc cctgcccgca ataaccagct
     2761 tgcactgagg tgcagtaaaa gcacacgcgc cgccgtgcgg cgagacagac aggccgatga
     2821 ggtttgtatg catgaatgat gggcacatgc ctgcggctta agcggtgtac tgcctgccgg
     2881 ggtcgattct ggaaatagct agggaacgtc tgaaaaatgt aggcggcgaa gttctggcta
     2941 ggcgaatttg gcaaaaaaag cggagtttac aagcagtaaa tgagcatttt tttgccaaat
     3001 tcaacgccgc cagggcgagt aaggtaattt ttcagaggtt ccctagtagc agctcctcct
     3061 tgcaactcct tagcaccccg gcggcaaaat agatcttcct catttattat cccgccgagt
     3121 taacaaataa gtgcggatta ccaattaatc agaatctccc gccaggtctc ccgtttaccc
     3181 tgtaccgcac ctgcagtgat ggtttgccag gtaatttgcc cagtggaggt ttgcactacc
     3241 gcattgcgca cgccgttaga gttttgatga atctgcggtg ccgaaggcgc gcccaatccg
     3301 agtgaagcgg acaaattcac taaatcctta ccattaaatt gcacactgga atccttacct
     3361 atgggggcaa aataaccggg aatacctgca aataaatgtg gcgcatttaa ataaccctgg
     3421 ccttcaggtt ggcattttaa gccgctgggt tgatattcgc tgaaaattaa tgactggtcg
     3481 gccactacgg atttattagt gttgcgcgag cgggcacgac tgaaattaaa ataccagcca
     3541 gatactttac tcacctcatc caccacatca taaaacgccc cagcagaacc gccattactt
     3601 aaggtaaccg ggcttgagcc agtggattta gagcgaacag caccatcggc gaaaacctga
     3661 atatcggtag tgttaattaa gctgctggtg gtgacggcgg cattcgtcat aacacccgcc
     3721 gctttaggct ctttaatccc gtaataactt tgttgtgaag atgaattatt gtcatctacc
     3781 acgaaaaatc gccctgtgcc aaaatatacc cagaaattat tcaggtgatc cgtcaaagtc
     3841 gttggcgctg cgcttacggg ttggttggta ttcatcacca aagaactggc aaatgtcact
     3901 gacgagccag acacagtggg caccacacgg aataatttac ccgatggcgc tgcgggcgtg
     3961 ccgctgaccg taccgaagta cagatcgtcg tcgacataat cctggttcca atccgccgct
     4021 gtaatgccgc caacaaatgc agcggtttgc acaccgtaca atgatgaatg gatcgatttt
     4081 gcttgcaaat cgtagataac aagagacgca ttaccggagg tttcagccgt atttaaggcc
     4141 ttagattttg tcgtggcatc ataaccacta ggccctgaac ccaacaccaa aaaccaacgg
     4201 ttaatagccg gagaagaata ggaaccatcg ccactgttag gtgcacgcat tttcataacg
     4261 gtaacatccg gggtagcaaa acccagcgtg taattggcaa gctcaccaat taataccggt
     4321 ggctgctcag gatcggtaac atccataatc acataaccgg aacgcagggt aatatcatcg
     4381 gcactactgg catctgggtc actattaggg tccagagtaa aatcgccacc accaaaacgc
     4441 ataccggcaa caataatcgt accccagccg ccggggtgag ttgcatccgg ggtaaaaata
     4501 ttcacatcat aactttttac cgggccgtcc atataataaa cgtgggggta atcagccttg
     4561 gctaaccatt gcaaatgggg taataaatta taaggaacat aagcccataa ttcggccccc
     4621 agcggatgct caacttcacc cttgcctgaa gataatttat aagtcagcgt atttggatcg
     4681 taaaaacctg cattaaacgc atgcaacata ccgtcgttag cgccaacata aaccacttgg
     4741 cggcgttttt catatttaag tttatattcc aagtaggtgt catcgccaaa cgaaacatcg
     4801 taactggaat taggcctgcc cacactgacg ggtgatgaat taactatatc gcccaataac
     4861 caaggcttag gctgcccggt gttggcgtaa tcaacggtgc gattgcggaa acccggcagg
     4921 ccttcttggc cgcgaataaa attcactaaa ttcggtgcat cggctttatt ggcactggtt
     4981 aagcccaata atcgatactg attgtcagta gcgccattgg ggaaagtggc tggcacaaaa
     5041 ggctcaacct cattcaccca agcatcccaa ccaatttgac catcgccagt gcggtcgata
     5101 ccggtaaaaa tatatcggcc actgctggca acagccgaat aactgcgctg agtagtaaga
     5161 tcggttaaag acgcaagatt actattggcg ctccaaatgg gttttaattt taaaatatca
     5221 tccgctgggc ccactgggct gccgcgcgcg ccagtattgg ttatttgata gcgctgaaag
     5281 taagtgcgtt ttgcataggg gtcataataa atatctaaaa tgctgtcatc cgccgttaag
     5341 gttccataag gcgcggcatt atcatcccgc atattacccc agcggtcgat aaaataagca
     5401 ttcagtgaac ccacccaatt gacattggta gcaccgctgg catctgtcac acggggatta
     5461 tacaaagcct gaaataaagc cccctcaccc gccccagtgt tggaaaccaa ggctgcagag
     5521 gtaccggatg atgtgcgctc tagaatactg attaaaatag aggttaaatt atttttaagc
     5581 tgcttaggat cgatggcaaa aaagtaatta tcgggaatgc catcggcgcc agtgttaccc
     5641 gtggaattgt tttttctatc ccactcggtt actaaatcag gcgtattaga cgcattggaa
     5701 tcgcgaaaac caccccattt agcggcataa aataatggct gttgcaacag gctcaccagg
     5761 gaaccggcta cagaggttgc acctaaagta aacgttttac tcgcagcggc ttgtgaaccg
     5821 gtgcaacctg aacctgtaca ttcatacccc acataactat ttataccgga taaagcgtgg
     5881 aaaccatctt cagtagtgcc actcaccaca aaaccgaacc ccatagcatt gggtgtcgat
     5941 tcgccaaata cgtcgctcgt gacagttaat gaagtgctgg taagcacata gctgatcaca
     6001 ccactcatat cctggtcata atcaccgccc tgttcgctgt cctcccaatt gacataaaaa
     6061 gtgccgcgcg cctcagtagc cgtcaccgat tggctaacaa ttttaaaatc cacaatggca
     6121 cagttaccgg acacactgtt gttgcggcaa gcaggtaata ttgaaaccac tttattatta
     6181 ctggggttaa ccaccgtaac cttaggcaag gcgggcgaga gtgtaacgcc gtaagtagaa
     6241 acactttgtt tatcgacaat atccgggcgt aaatcggcaa tatgggcttg ataggccagg
     6301 cccgccaaac ggtaagagcc atccaaacgg ggtgcatccg ggcaggtgcc ttcggccaag
     6361 cctaaatccg acacgagttt agcggtacat atttgattgt tatccgatga gcccgaaccg
     6421 ccattttcac cgacgaaaaa atatttaccg gtaatatttt caccatcccc cacagttttc
     6481 gtcagctgct gggccgtttt ggcagtggct aaatcggtaa ccttccctaa ttcatcgcca
     6541 tcgtaggaca ctgtactggc attaaaacca atcacattta aattcgcgca ggcggtggtg
     6601 gcattttgcg ggttggacca ggtggtctgc cgcgttaagc cagcaaaata actggtgtca
     6661 tcggcatcaa atgcgctatc gggggttttg cccgccatat agcggtaaca ttccagcata
     6721 atttccgcaa acgggttgcc ccagttggta cattcaccgt tgctaaagct atttttaccc
     6781 caattacagt tatcactgga attataagta ccctcggaac ctgaattaaa attgtaatta
     6841 agcaagcgca attgattgag cgagttgaca ataccgccag tggcgggtgc tgctttaaaa
     6901 gtaccatccg tagccacatt caactcatca ctaaatgttg aaacattttt gcgcaatacg
     6961 ccgcctgatt tattcttttt ataggtgcct gtcattaaac caaacaaggc cgagccatca
     7021 tcgccgaact gctgcagaat acctaccggt tttaaattac cgctgggata agacttgcaa
     7081 tcctcctgtc cgcgcagtgc ttcactgaca cacacctgca cttttactga ataatcgccc
     7141 accttaacgc catcgcgctg gggaacaacg gcactcgcag caggatttgt tgcggctgca
     7201 aaaataccgg ttgcatccgg gtcgttacca ttgccgccac ccgctgtgga ctcaccggta
     7261 aataaacact gcatgcgttc attggccgcc cacagcgaat aattaccact tacagcacgc
     7321 attaatggtg gagaagtcac cgcttgcgac agcaagacac cgctataacg cgttgtgtta
     7381 cacagcgtaa taccgcgcgc caaacggcac gctttatagg tggcataacc gggcgccgct
     7441 ttactcgctg gcgctgtgcc gcagctagcg gcaagcgtaa atggcgttaa tttttcaagg
     7501 tcatcgcctt cataatactt ggcaaaactg tgcgcatcat tggctaaata agagcgttct
     7561 aaaaccgtat cagtggcggt atcaatagcg cgcaagccgc cgtacaatac tttgcgcacc
     7621 tcatccacac gggtcatggt agcccaattt aaaaaattac cgctccaaag attagtcccc
     7681 gcacagtatt tatcggcagt gatactaata ggcacgaaat acttcagcgt agtatcgtac
     7741 tgataacact tataactatc gaaatacccg gcgtattcaa aagaattttt ataggtggta
     7801 tccactatgc catcagcatc taaatcgtca aaatcggtaa atgctttgta aaaaagctga
     7861 tgatcttttg acaccgccag catcaccata ggctttgcgc tttccgccaa tagcggaggt
     7921 acagcatcgt aatcactgac caccggcagc ccaaaggcca gggaatgcat tgccaataat
     7981 gatgcaatta acccactgcg catattaaaa catgtactgc gcattagcct taataaccta
     8041 gccataccac acctcaatca cagccccaca gggcgtgttt acacgcaaaa aaataatgaa
     8101 tgttctaaag attaattagc aaaacctaac agcacctgct tagtgcccag ccaaaacctt
     8161 ataggttgat tgcaacataa cgcgcccgcg cagaccattg ccctccgcaa aggcggtgat
     8221 acgaaacaaa ggcgcaccgt tgttcttttc cccggccgca ccagaaaacg gtgtggtggc
     8281 gtcgcgcttc acgtaacata aaaattcagt gatgtatttg acatcagcgt ctaggcgttc
     8341 aattttaatt ttcttataac gcgatgccgt tttccaaata ttcagcgtgg catcgctcca
     8401 gaaatcgata acagcaattt tgccggtggg gtcactcacc ttacaatcaa actcggaatc
     8461 aacattaata taggtgcctt gaaaacacag gccatcattg caggttttat taaaacaacc
     8521 ggtgccagtg caggtactga gtaacttttc tcgatcgggt ggcgacatag ctagttgctg
     8581 ttcaacttgt gttaatgccg cctcagctgc ggcaaatgcc gtggcatgat cttgagccgc
     8641 cgtggccatt tttaattctg aggtggaatt ttgcatgccg gtgacaaccg ccagtaacac
     8701 caggctcacc actaaagcag cgccgcgctg actgccgatt gttgttaggc gcttaggttg
     8761 caatgtcata ttcagttagc ctcaaactcg cggcgggtag ttggcgttaa aagcacaatt
     8821 gcacggtaat ttacatgcga ttgcgaataa ccacggtaga attcaccgcc tggcgcaaat
     8881 agcggtcatt gtaggtaaca ccattaataa cctgtgtttg attgctgggt gcaacaggat
     8941 tttgcgaacg aaagaccaag gaaatacgca cgctcttaac cttatcccaa tctttgccat
     9001 ctatagcacc gctgccatct acatccattt gcgctgcggt ccggtactgg tttacatcgc
     9061 cgtcggcatt agtatctaca ccataaagca actgcatatc ctccacgcct tgggcaattt
     9121 cttccgatcg tgtggttagg gtgctatcca tcaccaacga ctggcgtttt aaagcaggca
     9181 tatccgccat aactgtagaa gggccaataa aataggcgcg ggagataaag cgcatgactt
     9241 ttgaaccggg gctataactg ccagtggtag tgccataacc attgcaactg acagccgtac
     9301 aactcggtgt acatacaaaa ctgccgcgaa taattttggt gcaattgcta gttaccgcac
     9361 ctgtattgtg aacaattttg tttgcaggca acgcattcgc attaggtgaa ctcacttgaa
     9421 ataaacccac gtaacggcaa ttggcgtcag caatcattaa aggcgtaccc ggtgggaagc
     9481 tgtgttgctt ataaagcgaa atagttgccg aggccgccac atgtgattca acacctaatt
     9541 catcactgtc gccacggcgt acaataaagg catcactacc cacctttgcc agggtgttca
     9601 gagggctggg aaatccagcc acactggttt gaccatcgta gccttcaatg cccccggtgc
     9661 cgacaaaatt aaaaaaatta ccatcaatcg aattggcaat taatgaggtg gtaggcgatg
     9721 cacaacccat atagcccgcc atgcgaattt ctgaagtaag cgtttccact acgaagcggg
     9781 cattatcctg aataaatgca acttcatctt gctgcatttt attggttttt gatttcacca
     9841 ccacattgat aacgcccgag agaatgatca aaccaatggt tactgcaatc agcagctcaa
     9901 caatagaaaa accttgcgca tttgccgcag atgcaggatg cgaaaaagac gagtgcttgg
     9961 gtaaataatt tatgcgcgta tccatggctt tacacatcgg taaccaagac gtattcaaag
    10021 aatttttcac tgccatccac ctgggcgcga gcgtcgttaa agcgcacggt aattacggtg
    10081 ttgtcgccca cggcttgaat tgaggcctta ccctggggta aaaatcgcgc tacgttagtc
    10141 aaccatttat ttaaattcca atcccgcagc tcacctgcac tgcatgaaat agttgtgctc
    10201 tcacaggttt tggcattggg aatagggtcg tcaaaaagaa tgttatagcc gcctgccacg
    10261 gcaggtgttg tgcgcatggc atcggcaatg tcgttggcta aaaataccgc ctgggcataa
    10321 taataagtgc tttgaccata ttggcttgaa cgcgcctgca tggctaaaac ccctaataac
    10381 cccacaccca agaccaccag cgccaccata acctccaata gtgaagatcc gtgttggcgt
    10441 gtatttcctt tcggtttcag ctcagtacgc ataatcgtat ctcgattgac tcatccactc
    10501 ataacactag agcaggattt atgcggtcgc cacgggcaat gacgggcggt gtcaattatt
    10561 gacaagctgc gcacactcag aaatcaagcg gccgctttat tattaaaaaa taaaataacg
    10621 atcgtttgat tataagtgca tgggcatcta atgggacacg aggattaacg gtcattttct
    10681 tcgatacata ggttgtgatt tatcgataca cagttgccta tcaaccagta cttaatccgg
    10741 catacaccgg cgcctcggtt acagccagat tttaaatatg agtgcgcaaa attgattaac
    10801 tgcgctataa gcggcgccga aaatttggcc catcgaaaca aatttatctt tgatcaaagg
    10861 agcgcgccaa atttccgaca agctataaaa acccctttga tacatcaata gcagataaat
    10921 cgaaggtggt gaaccttgct aaatca
//
LOCUS       DAOQGS010000064        10905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2807_length_10905_cov_5.635392, whole genome shotgun sequence.
ACCESSION   DAOQGS010000064 DAOQGS010000000
VERSION     DAOQGS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2807_length_10905_cov_5.635392"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            812..2701
                     /locus_tag="PKZ52_03820"
     CDS             812..2701
                     /locus_tag="PKZ52_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HNG58929.1"
                     /translation="MLHNSLIRAISPKLFGSIVVASSIALLTACGGTTSSSSSSSSSS
                     SSSSSSTGGNNSSTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGIGSSDTAVVDPA
                     TYTGSIALTQQAKGKPGASLQTTSNPFADSYFYLSPDISTMMDYSLKQVSDASVIDKI
                     KYIQRQPSAIWMDSTATIYGDPTAGRRSLEGHLDAAVAQQKFFEMKDGQKSPMTIVII
                     IYNLPDRDCAAFASNGQLYEIGKPKNPQPNNGMTKYRDEYIRGITAVLSKKQEYKDLR
                     IVALLEPDSFPNMITNTKLNPDGPSLVWPELTTANGTTYCDTLLTYSSPGLATGLGVY
                     GRGLQIAIEELYKVGNVYTYLDIGHAGWLGWDDSANNQSNLKRAVAGFQSLIKGANTD
                     GIDGFKKVKGFASNTSGYTPVEEPLISNAEADRQALQDWYEWNKSVDELSYIDAFNTR
                     FRAQQPGFQPGFIIDTARNGWGFANRPKPGTGTKGTDSSKRVDKRAHRGHWCNVNNAG
                     VGEVPKAAPISSKSYLHAFFWMKPPGEADGISFDVTKYPKGSAAYNALDAIDKAVVDS
                     ASDPKYAGKALDTMCTPGGIRDGKKEVNVVPALSPHAGGWFHKQFLMLINNAYPALGT
                     SDYGK"
     gene            complement(3027..3590)
                     /locus_tag="PKZ52_03825"
     CDS             complement(3027..3590)
                     /locus_tag="PKZ52_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58930.1"
                     /translation="MLLDEYQKLERLQNTGVKGATTEANTFAFRGDLVLEQGDIADAQ
                     GRRLPPKSVVKQAALLITNDKIRFLCGNVHDLAGLEMLHGLYAEDYAPDICLFMYAEN
                     IKEKIKVSLNGFTYTCLPIEEMAVWSALMDDLRLDKEDFKGCSNEDKVIVMYRGAVDF
                     QVKGDVLPFSDALGLRVAATKSQRGPV"
     gene            complement(3784..5775)
                     /locus_tag="PKZ52_03830"
     CDS             complement(3784..5775)
                     /locus_tag="PKZ52_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNG58931.1"
                     /translation="MSAHLFLRPQNFYLMRRRIFLRAAQVCSCLMLALLSPVSFADVK
                     LPRLISDGLIIQRDAPIRIWGWADEGEQVQVTLGDKTQTVSTKAGIWQAQFKALPANT
                     QVNIDIQGKNHLRVINVVAGDVWIAAGQSNMETNLNRVAPRYPDLINRTQLPMIREFR
                     VPVAYSFKGPQADFTQGQWKTALPHNLPEFSAVGFFFARDLFAQLNVPIGIILIAVGG
                     SPAEAWMSTEALKAYPHYLAAYSRFTDDSALQNTLAQDKARVDAWYQNLNAEDPGLKA
                     NPAWFSQQVNFATWPTLQVPGMWQAQGIDFKNGVTWLKKNIQLTPAQTQQAATLWLGT
                     LVDADQVYINGEAIGQTAYRYPPRIYTVKPGILKAGINTITVRLTSTSAEPGFVPDKP
                     YKLQLGSEEVNLTGQWHYQIGHRAGAYPSTTTLHYQPASLFNAKLAPLLKTPVKGVLW
                     YQGESNTGRAEEYAGLFADLIRDWRQQFAKPKLPFLFVQLPNFLAANSAPSESQWAQT
                     REAQRQALKLPHTAMAVAIDVGEWNDIHPLHKQPVGERLSLLAQSLVYGNKDLIAQSP
                     MPVSAKAKKGQVVIKFASANNALQLCSGEQLSHFALAGADKKFVWASAKIINNTVVAR
                     DERIGAPKFVRYAWADNPQGANLCGAGHLPASPFELPVH"
     gene            complement(5845..6144)
                     /locus_tag="PKZ52_03835"
     CDS             complement(5845..6144)
                     /locus_tag="PKZ52_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HNG58932.1"
                     /translation="MTDNAELENRYWDLVDQLIGQANEACEHLDPSAVSAALLNAAAR
                     FNAFAVATASLDRNEFSEEIEPALQHLTGRYRELLRDNLEDYREHYKVYIRPNEA"
     gene            complement(6366..6779)
                     /locus_tag="PKZ52_03840"
     CDS             complement(6366..6779)
                     /locus_tag="PKZ52_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HNG58933.1"
                     /translation="MRQDSLYGKFAKRTANLCGKPPTFALAVAVIVVWLVSGPLFHFS
                     DTWQLVINTGTTIITFLMVFLIQNTQNRDTEALQLKLDELIRATRGAHNALLDLEELE
                     LADMEDFKQRYIQLAKHARAALESGEDDTDTPEAV"
     gene            complement(7077..7928)
                     /locus_tag="PKZ52_03845"
     CDS             complement(7077..7928)
                     /locus_tag="PKZ52_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG58934.1"
                     /translation="MESSEAEFIIGDMHFAAKHWGNPAGKKILALHGWLDNAASFDFL
                     APLLPDYHWVCLDLAGQGKSDHRPHFGAYNIWQDVTEVFAVADALGWERFSLVGHSRG
                     AVIAGLACGTFPERIEHLALIEGVASFISREREAPELLASAYLSLKTQAARHQSMHPS
                     LEAAIAVRVDGKFPVSLADAQALAARGVEPFEGGYTWAYDYKLLANSEVRFTLGQLNA
                     FFARIKQPVHLLIAEQGIIIQHAEVQRWLATQTHICQTQLPGGHHLHMSENYQAVGQW
                     LRQVLNS"
     gene            complement(8022..8480)
                     /gene="sixA"
                     /locus_tag="PKZ52_03850"
     CDS             complement(8022..8480)
                     /gene="sixA"
                     /locus_tag="PKZ52_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNG58935.1"
                     /translation="MELFILRHAEAGLDAATDFDRTLTELGKHQLSQVVGRHQEALAS
                     ISQVWVSPYVRTQQTLAQIRHWLPAHVPIETTEALTPESPVRGLLPLLERSSGQRVLL
                     VSHQPLVGEAVAKLCGLEPGAYRLGTSALTHIELPLVGFGQGRVRFCDQP"
     gene            complement(8483..8758)
                     /locus_tag="PKZ52_03855"
     CDS             complement(8483..8758)
                     /locus_tag="PKZ52_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNG58936.1"
                     /translation="MRLIYMLLFAVCLQVASFVMWVVVIVQFVFALITGEDNSNLRHF
                     GRCLSRYIHQSLLFLTYNSEAKPFPFSDWPTCQDDAPEPKLPPSDTL"
     gene            complement(8908..9933)
                     /locus_tag="PKZ52_03860"
     CDS             complement(8908..9933)
                     /locus_tag="PKZ52_03860"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNG58937.1"
                     /translation="MLDASSSLVYRVAVLGGGSFGTALANIIARNGHTTHLWMRTQEQ
                     VLRCQQEGENSKYLPGYALEPSLMLTSDLAASLKGADIVVIAIPSQSFRSVAAALAPL
                     LPEHALVVSTTKGLEGDGFTLMSEVLRQELPGARIGVLSGPNFAKEIIQNQYTASVVA
                     SPYPEVCERLPKVLSSPSFRVYSNPDMFGVELGGALKNIYAVITGMAAAQGCGQNTLA
                     LILTRSLAEMSRFANKLGANPKTFLGLAGMGDLILTCTSDLSRNYRVGYAIGQGASLE
                     QALGKIGGQVAEGINTLKIVHEKARALDVYMPLVRGLHAVIFEGQELSLIVRGLMTAA
                     AASDVET"
     gene            complement(10084..>10905)
                     /locus_tag="PKZ52_03865"
     CDS             complement(10084..>10905)
                     /locus_tag="PKZ52_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58938.1"
                     /translation="PCPTGVYKTYSNQCASDAAKAKFISNGECGELEGKPYIEEPTAC
                     TLQYDPVCAATTDNAPCKTIPCPAVVHKTFGNACEARVAKASVIQTGECGKLEGTPVT
                     KLSGACTADAPGACAKTTSTIVCVTTPCPTHVYTTFSNRCEAERVLATVIGDGPCGSL
                     QGVTAGGEPPVKLVDSLPSPSSAKAANVHFKGDVLTLTLGYSGCGPQHFDLYIGKAFL
                     KSKPVQVNFVFKAQREETCDAYFTTEFSYDLLPLKQLYGAEHGEIVLPGIGSYVF"
BASE COUNT         2557 a         2913 c         2906 g         2529 t
ORIGIN      
        1 attgctctac ctcctgcatc catgcagtcg tcggcaattg ctcctgcatt gctctgcatc
       61 catcaatttt gaacaaacct acacgtacat ctgcgcactg cgcatacgga cttaattagc
      121 ggccccttat caacctgccc cagataaggc gcgtgcttat taagggcttt attttgcaaa
      181 taaattacgc gcagatatgg caagcaagag acgctgtgat gaggtgcgcc acggcacggt
      241 aaaacactca ccgcgcataa ctcggcgaat gcgcaacgat aatcgtctga gacacaagtg
      301 accgacacgg ttgtggccca gctcatacca ttactgccgt gctgcaagcg cacatttgag
      361 gatgcgctga taaagcctga attcggcaat agtgtaaaaa cagacaaggt gctcccttca
      421 gcttcatcgc gaatttccta gctgcggcgg ctgcaaatac ccgcactgcc gactggatca
      481 gaaattcctt aaaccttact aaaactgcgc tgtggttcta gcaggtaaat gaatttctgc
      541 tcggcgcgca atatgacaca ggtcgctttt ctcaccgaaa tcctatcaaa caccccctat
      601 gcaaacccct aaagtgtgac gacaccgacg gaacccaggt gtattcgctg agacaataag
      661 cccaggtgga cttttcccag ttcatttttc gcagcactgc aggtgatagg ctcaattttc
      721 ccgacagttt gtgggtacag gtggggcgca ttgatcgata ccagcaatct tggctgtcta
      781 aataacaaaa acgttttttg cggagaaaaa catgttgcac aactctctta ttcgcgcgat
      841 cagccccaag ctgttcggca gtattgtggt ggcgagctcg atcgcactgc tcactgcttg
      901 cggcggtact acttcaagta gctcttccag ctcttcttct agtagctcgt caagctcttc
      961 aactggcggc aacaactcta gtacgagctc atcgtcttcc agttcatctt ctagctctag
     1021 ctcttcttct agctccagct ccagttcttc ttctagctcc agctcatcca gcagttccgg
     1081 tatcggcagc tcagatacag ctgtagtgga tccagcaacc tacaccggta gtatcgctct
     1141 gacccaacaa gccaagggca agccaggcgc cagtttgcaa actaccagca acccattcgc
     1201 tgactcctac ttctacctga gcccagacat cagcaccatg atggattact cgctcaagca
     1261 agttagcgat gcctccgtga tcgacaagat caaatatatc cagcgccagc ctagcgctat
     1321 ctggatggac tctactgcca ctatttacgg tgatccaacc gctggccgac gctcattaga
     1381 aggccacttg gatgccgctg ttgctcagca gaagttcttt gagatgaagg acggccaaaa
     1441 gtcgcccatg accatcgtaa tcatcatcta caacctgcct gatcgcgact gcgccgcctt
     1501 tgcatccaat ggccagctgt atgaaatcgg taaacccaaa aatcctcaac ctaacaacgg
     1561 catgaccaaa taccgcgatg agtatatccg cggcattacc gctgttttga gcaaaaagca
     1621 ggagtacaaa gacctgcgta tcgttgctct gttggagccg gattcattcc ccaacatgat
     1681 caccaatacc aaattgaacc cagacgggcc ttctctggta tggcctgaac tgactaccgc
     1741 taatggcacc acctactgcg acactctgtt gacctattct tctccaggcc tggcaactgg
     1801 ccttggtgta tacggtcgcg gtctgcaaat cgctatcgaa gagttgtata aagttggcaa
     1861 cgtctacacc tacttggata tcggccacgc tggctggtta ggttgggatg actctgcaaa
     1921 caatcaaagc aacctgaagc gcgctgttgc tggcttccag agcttgatta aaggcgccaa
     1981 caccgacggc atcgacggct tcaaaaaagt taaaggtttt gcctctaaca cctctggtta
     2041 cacgccagta gaagaaccgc tgatttctaa cgcagaagct gaccgtcaag ccctacaaga
     2101 ttggtacgag tggaacaagt cagttgacga gctgagctat atcgacgcct tcaacactcg
     2161 tttccgcgca caacaacccg gtttccaacc cggcttcatc atcgacaccg cgcgtaacgg
     2221 ttggggcttt gccaatcgtc ctaagcccgg cactggcacc aaaggcaccg acagcagcaa
     2281 gcgtgttgac aaacgcgctc accgcggcca ctggtgtaac gtgaacaatg ccggtgtagg
     2341 tgaagtgcct aaggctgcgc caatttctag caaatcctac ttgcacgcat tcttctggat
     2401 gaagccaccg ggtgaagcag acggtatctc ctttgacgta accaaatatc cgaagggctc
     2461 tgccgcttac aacgctcttg acgcaattga caaagcggtt gttgatagcg cttccgatcc
     2521 taagtacgca ggcaaggcgc tggacaccat gtgtactcct ggcggcattc gtgacggcaa
     2581 gaaagaagtg aacgtggttc ctgctctgtc acctcatgcc ggtggctggt tccacaagca
     2641 attcctgatg ctgatcaaca atgcttaccc agcacttgga acaagcgatt acggcaagta
     2701 atggctgaat ctggctaaca aaaacccctt ggcaaaacca aggggttttt tttggactgc
     2761 gcttttgggt ttaagggttt ttggagcctg cacgcactaa aattcccctc ctagggaggg
     2821 gtagcaggct tacctgcagg ggttacggtg gcgggcgctg ttttttgagg cgagggaatt
     2881 tttaaccttt agggccgcga ataaacccaa gccgagacct accaccccac cccttcgggg
     2941 cacctctcca aggaggggaa ttttaggccc tgcccgtggc caaaaacggc cgccgggcta
     3001 aaagcgcagt gagctctgct agggttttaa accgggccgc gctggctttt ggttgccgcc
     3061 acccgcaagc cgagggcatc gctaaagggc agcacatcgc ctttcacttg aaaatccacc
     3121 gcgccgcgat acatcacaat gaccttgtct tcattgctgc agcctttaaa gtcttcttta
     3181 tccaaacgta aatcatccat taaggccgac cagaccgcca tttcttcaat gggtaggcag
     3241 gtgtaagtga aaccgttgag ggaaactttg attttttcct taatattttc ggcgtacata
     3301 aataggcaga tgtcgggcgc ataatcctct gcatacaggc cgtggagcat ttctaagcct
     3361 gccaagtcgt gcacattgcc gcataaaaaa cgaattttat cgttggtaat taatagcgcc
     3421 gcctgtttta ccacggattt aggcggcagc cgtcggccct gggcgtcggc aatatccccc
     3481 tgctctaaca ctaaatcacc gcgaaaggca aaagtgttcg cttccgtggt ggcgcctttc
     3541 acgccggtat tttgtagacg ttctaatttt tggtattcat cgagcaacat aaaaaacctt
     3601 tggcctaata tcagtaaatt ggtatgtggc ctcagcaata tatgtgcgca attgatgggt
     3661 tgttgtttgc agcctaaaaa taggagtttt gtacccacag gcacccccac ggcatgtcgc
     3721 attcgcaaca aaggttgtgc aatggtttaa gccttgtggt gaatgcgaca aaccggcggg
     3781 agattaatgg actggcaatt caaagggcga tgcgggtaaa tgcccggcgc cacacaggtt
     3841 ggcaccctgg gggttatccg cccaggcata acggacaaat ttaggcgcgc caatacgctc
     3901 atcgcgtgca actaccgtgt tgttaattat tttagcgctg gcccaaacga attttttatc
     3961 cgccccagca agcgcaaaat ggcttaattg ttcaccacta cacagctgta gcgcattatt
     4021 agcactagcg aatttaatca ctacttggcc tttttttgct ttggcgctga ccggcattgg
     4081 gctttgggcg attaaatctt tgttgccgta caccaggctt tgtgccagca ggcttaagcg
     4141 ctcgccaacc ggctgcttgt gtaatgggtg aatatcgttc cactcgccca catctatggc
     4201 caccgccata gccgtatgag gcagttttaa tgcctgccgc tgcgcctcgc gggtttgcgc
     4261 ccattggctt tcactcggcg cgctgttggc cgccaaaaaa ttgggtaatt gcacaaataa
     4321 aaacggcagt tttggcttgg caaattgctg gcgccaatcg cgaattaaat ccgcaaataa
     4381 accggcatat tcctcagcgc gcccggtatt ggattcgcct tgataccaga gcacaccttt
     4441 taccggagtt tttaacagag gcgctaattt ggcattaaat aaactggccg gctggtagtg
     4501 gagggtggtg gtgctggggt aggccccagc gcgatgtcca atttggtagt gccactggcc
     4561 ggttaaatta acctcttcac tgcctaattg cagcttatag ggtttatccg gcacaaaacc
     4621 cggctcggcg gaggtgctgg tgaggcgcac cgttatagtg ttgataccgg cttttaaaat
     4681 gccgggtttt accgtgtaaa tgcgcggcgg gtagcggtac gcagtttggc ctatggcctc
     4741 gccattaata taaacctgat cggcatctac tagtgtgccc aaccataaag tggcagcttg
     4801 ctgcgtttgg gcgggggtga gttgaatatt ttttttaagc caggtgacac catttttaaa
     4861 atcaatgccc tgcgcttgcc acataccagg aacctgaagt gtgggccaag tggcgaaatt
     4921 aacttgctgg ctaaaccacg ccgggttggc ttttaggcct gggtcttcgg cattaagatt
     4981 ttggtaccac gcatccacgc gggccttatc ctgggccagt gtgttttgta gcgccgaatc
     5041 gtcggtgaat cggctgtaag ccgctaaata atgcgggtag gctttaaggg cctcagtgct
     5101 catccaggct tcagcaggag agccgccgac tgcgatgagg ataatgccta taggcacatt
     5161 taattgcgca aataaatcgc gcgcgaaaaa aaatcctacg gcagaaaatt cgggcaaatt
     5221 gtgcggcagg gctgttttcc actggccctg ggtaaaatcc gcctgggggc ctttaaatga
     5281 ataggccacc ggcacgcgaa attcgcgaat catcggcaac tgagtgcggt taattaaatc
     5341 tgggtaacgg ggcgccaccc gattgaggtt ggtttccata ttcgattgcc cggcggcaat
     5401 ccacacatca ccggccacca cattaatcac gcgcagatga tttttgccct gaatatcaat
     5461 attcacctgg gtgttagcag gcagcgcttt aaattgggcc tgccaaatac ccgccttagt
     5521 actcacggtt tgggttttat cgcccaaggt aacctgcacc tgctcgcctt catccgccca
     5581 gccccagatg cgaatgggcg catcgcgctg gataattaac ccgtcggaaa ttagccgcgg
     5641 taatttaaca tcggcaaaac tcaccgggct caaaagtgcg agcattaaac agctgcacac
     5701 ttgggccgca cgcaaaaaaa tgcggcgacg cattagataa aaattctgtg ggcgcaaaaa
     5761 taagtgtgcg gacattaaaa accctcgcgt ttttataggg cggaacggat tagcgtgctc
     5821 ggggggggga taaaggcgct gcaatcaggc ctcattgggg cgaatataaa ccttatagtg
     5881 ctcgcgataa tcctccaaat tatcgcgcaa taattcccga tagcggccag ttaaatgctg
     5941 tagggcaggc tctatttcct cactaaattc attgcgatct aaggatgcgg tagcgacggc
     6001 aaacgcgtta aaacgagcgg cggcgttgag tagggcggca cttacagcgc tgggatccag
     6061 atgctcgcag gcttcattgg cctggccaat taattgatcc actaaatccc agtagcgatt
     6121 ttctaattcg gcattatcgg tcatgtttat ttattccatt gtaactattt agcatctttt
     6181 tatgtatttg gcggtaattc ccgcgcttgt tggcaactat cgcatgcgtt tttttgtgcg
     6241 gtaagctctc gatacttcac ccgcatagat gactacaggt tagtgcagac acctctgcta
     6301 caccgcgaat cgattgtgcc caggtgtgct gaatcgttac attccagttt aactgggcac
     6361 atggtttaca cagcttcggg ggtatctgtg tcgtcttcac cgctttccag ggccgcgcgg
     6421 gcgtgtttgg ctagttgaat atagcgctgt ttaaaatctt ccatatcagc cagctccagc
     6481 tcttctaaat cgagcagtgc attgtgggcg ccgcgggttg cgcgaattaa ttcatcgagt
     6541 tttaactgca gcgcttcggt atcgcggttt tgggtatttt gaattaaaaa taccattaaa
     6601 aatgtgatga tggtggtgcc ggtgttaatc accaattgcc aggtatcgct gaaatgaaat
     6661 agcgggccgc tcaccagcca gaccacaatg acagctacag caagggcaaa ggtgggcggt
     6721 ttgccgcaca ggttggcggt gcgtttggca aattttccgt acagactatc ttggcgcatg
     6781 ggcatctcac cgatgggttg aacctaaaaa ccgcaattaa accctactgc agttcttggg
     6841 ttaaaaacag atttttaagt attaacccgg cggtgcaaac cgccgtgagc tgcgctcaaa
     6901 caaggaacgc attttcgcca gttttagggc agcgaaggct aaatagatca ggacgatcta
     6961 tttagccgcc gggttaatag gtgcgccaag gttaacggtt attactccta aaatgcacat
     7021 acgctctgcg tcttaagcac tctggctaaa cagccaaaaa acctggtgcg caattattaa
     7081 ctgtttaaca cctgccgcaa ccactggccc acggcctggt agttttcgct catatgcaaa
     7141 tggtggccgc cgggtaattg ggtttggcag atatgggttt gagtggcgag ccagcgctgt
     7201 acctcggcgt gctggataat aatgccctgc tcggcaatta acagatgcac cggctgtttg
     7261 atgcgggcaa aaaaggcgtt gagctggccg agggtgaagc gcacctcgga attggccagc
     7321 aatttgtagt cgtaggccca ggtgtaaccg ccctcaaacg gctccacacc gcgggcggca
     7381 agggcctggg catcggcgag ggagaccggg aatttaccgt ccacccgcac ggcgatggcg
     7441 gcctcaaggc ttgggtgcat agactggtgg cgggccgctt gggttttcag gcttaaatag
     7501 gcgctggcca gcaactctgg cgcctcccgc tcccggctga tgaagctagc caccccttcg
     7561 atcagcgcta ggtgttcgat gcgctcggga aaggtgccac aggcgaggcc tgcaatcacc
     7621 gcaccgcggg aatgccccac caggctaaag cgctcccagc cgagggcgtc ggcaacggca
     7681 aacacttcgg tcacatcctg ccaaatgttg taggcgccaa agtgggggcg atgatcactc
     7741 ttgccttggc cggccaagtc caaacacacc cagtggtagt ccggcagcag tggcgctaaa
     7801 aagtcaaaac tggcggcgtt atccagccag ccgtgcaggg cgagaatttt tttgcccgcc
     7861 gggttgcccc agtgtttagc ggcgaaatgc atgtcgccga tgataaattc tgcttcgctg
     7921 gattccatgg ggctcttgtc gttggtctgg ggcctaagcc ccatcgaggg gaaaaattac
     7981 ggccgcagct tcaagctgca ggccttcagg gggcaaccag attatggctg atcacaaaag
     8041 cgcacacggc cctggccaaa ccccactagg ggcagctcta tatgggtgag tgcgctggta
     8101 cccaagcggt acgcgccggg ctccagtccg cagagtttag ccaccgcctc ccccaccagg
     8161 ggttgatggc tgaccagtaa cacccgctgg ccgctcgagc gctccaataa aggcagcagg
     8221 ccgcgcaccg ggctttccgg ggtgagggcc tcggtggttt caatgggcac atgggcgggt
     8281 aaccagtggc ggatctgggc cagagtctgc tgggtgcgca cataggggct cacccacacc
     8341 tggctgatgc tggccaaagc ctcttgatgg cgccccacca cctgactgag ctggtgtttg
     8401 cccaactcag tcagggtacg gtcaaaatcc gtagcagcat ccagccccgc ttcagcgtgg
     8461 cgcagaataa acagctccac ggttagaggg tatcgctggg gggcaatttg ggttcggggg
     8521 cgtcgtcctg gcaggtgggc cagtcggaaa agggaaaggg tttggcttcg ctgttatagg
     8581 ttaaaaacag cagggactgg tgaatataac gggataagca gcggccgaag tggcgcaggt
     8641 tggaattgtc ttcgccggtg atgagggcaa acacaaattg cacaatcacc accacccaca
     8701 tgacaaacga ggccacctgt aaacacaccg cgaacagcaa catataaatc aggcgcatcc
     8761 agtgggtggg cgataaaaga ttgtttttaa tttgttggtt atccacggcg aagtcctctt
     8821 aaggatgggg tgcggttcat tattaaggca gttatttttc ggtacgtcta gccattggcg
     8881 caggctgcgg gcaaatcggc gcccccatca ggtttccaca tcgctggcgg cggcggccgt
     8941 catcaacccg cgcactatca gggagagttc ctggccctcg aagatcacgg cgtgtaggcc
     9001 gcgcactaag ggcatgtata cgtccagggc gcgggctttt tcatggacaa tctttagcgt
     9061 gttaatgcct tcggccacct ggccaccgat tttacccagg gcttgctcca aacttgcgcc
     9121 ctggcctatg gcatagccca cccggtaatt gcggctgagg tcggaagtgc aggtgagaat
     9181 taaatccccc atgcccgcaa gccccaaaaa ggttttcgga ttggcgccga gcttgttggc
     9241 gaagcggctc atttccgcca ggctgcgggt gaggattaac gccagggtat tctgcccaca
     9301 gccctgggcg gcggccatgc cggtgataac ggcatagata ttttttaagg cgccgcccag
     9361 ctccacgccg aacatatccg ggttgctgta cacccggaag gagggcgagg acaacacctt
     9421 cggcagccgc tcgcagactt cgggataggg gctggctacc acgcttgcgg tgtattggtt
     9481 ttgaataatt tctttggcga agttaggccc gctcaacaca ccgatgcggg cgccgggcaa
     9541 ctcctgccgc aacacttcgc tcatcagcgt aaaaccatcc ccctctaggc ccttagtggt
     9601 actcaccact aaggcatgtt ccggcaataa aggcgctagg gccgcggcca cggagcgaaa
     9661 ggactggctg ggaatggcga tcacgactat gtcggcgccc ttaaggctgg cggcaagatc
     9721 gctggttaac ataaggctcg gctccagggc gtaaccgggc aggtatttgc tgttttcgcc
     9781 ctcctgctga cagcgcagca cctgctcttg ggtgcgcatc cacaggtggg tggtgtgccc
     9841 gttgcgggcg attatattgg ccagggcggt gccaaaactg ccgccgccga gcacagcgac
     9901 gcgatagaca agggatgaag aagcatcaag cataggatct ataaagtatt tttatttagc
     9961 tgcgcagtgt acccgctgcg ccgccaacct aaaacccgcc ccgtgcacag gcctacaaga
    10021 ctgttacagg cgggcaaaaa aaagccgggc taggcccggc aaggtgcaac cacattggca
    10081 gaattaaaaa acgtaggaac ctatacccgg cagcacaatt tccccatgct cagcgccgta
    10141 gagttgtttt aaaggcaata aatcgtagct aaattcagtg gtgaagtacg catcgcaggt
    10201 ctcctcccgc tgggctttaa acacaaaatt cacctgcacc ggtttgctct ttaaaaaggc
    10261 tttgccgata tacaaatcaa aatgctgggg gccgcagccg ctgtagccga gggtaagcgt
    10321 taagacatcg cctttaaagt gcacgttagc tgccttggca gaagaagggc tgggcaggct
    10381 atccaccaat ttcactggcg gctcgccgcc tgcagtcacg ccttgcaggc tgccgcaggg
    10441 gccatcgcct ataacggtgg ccaacacacg ctcagcctcg cagcggttgc tgaaggttgt
    10501 gtacacatgg gtcgggcacg gcgtggtcac acagacgata gtactagtgg ttttcgcgca
    10561 tgcgcccggt gcatccgcag tacaggcgcc actcaatttg gtgaccggtg tgccctctaa
    10621 tttgccgcat tcgccagtct gaataacgga cgctttggcg acgcgagcct cacaggcatt
    10681 gccaaaggtt ttatggacga cggccgggca gggaatggtt ttgcagggtg cgttatcggt
    10741 ggtcgcggcg cacaccgggt cgtactgcag ggtgcaagcg gtgggctctt caatataggg
    10801 tttgccctct aactccccgc actcgccatt gctgataaat ttggctttgg cggcatcaga
    10861 ggcgcactgg ttggaatagg ttttatacac accggtgggg caagg
//
LOCUS       DAOQGS010000065        10899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2810_length_10899_cov_4.907414, whole genome shotgun sequence.
ACCESSION   DAOQGS010000065 DAOQGS010000000
VERSION     DAOQGS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_10899_cov_4.907414"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1165
                     /locus_tag="PKZ52_03870"
     CDS             128..1165
                     /locus_tag="PKZ52_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNG58939.1"
                     /translation="MSSNTPVIPPGYGEWLTQLKADIAQSRNRAALAVNGELIQLYGR
                     IGRDILERQQAQGWGAKVIDRLANDLKTAFPDMRGFSSRNLKYMAFFAQLCPTLQFGQ
                     QPAAQLPWFHIVTLLTKVNTEAAREWYAQQAVKSGWSRSTLEINIKNQLQQRQGAAVT
                     NFAARMPATDSALAVETLKDPYLFDFLGLGNDAHEREIENGLIQHITRFLLELGAGFA
                     FVGRQFRLEVAGDEFFIDLLFYHTRLKAYVVVELKATDFKPEHAGQLNFYLAAVDAQI
                     KADDDKPTIGLLLCKQQNRLVAEYALSGIDKPIGIAQYQLLRELPEILVKSLPSIEEI
                     EAEFAASLPDD"
     gene            1237..6195
                     /locus_tag="PKZ52_03875"
     CDS             1237..6195
                     /locus_tag="PKZ52_03875"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNG58940.1"
                     /translation="MSALQTLLDTFRNTALTEREKGAYFESLVKVYLLNEPSYKDLFN
                     GKVFLWEEWRKYWIAQGNSDPQSDAGIDLVAVEDVAENPRIFAIQAKFYAEDSKIRKG
                     DGIDSFLSAMGKNPYTNGLLFITTSKASTHVIEGMKKRDKPVNIISLFDLESSQIDWS
                     QYQSTKAPVLKAKKSLRAHQSEALTNVLEGFKTVDRGKLIMACGTGKTFTSLKIAEAV
                     AGKDKRVLFLVPSLSLLSQTLTEWTQESTTPLHSFAVCSDSDVGKKRGKDEDAVQMLM
                     HELRYPATTEPARLAAEMTKRHDADHMSVVFSTYHSIEVISKAQQQFNLADFDLIVCD
                     EAHRTTGATFDGDDESAFVKVHDANFIRAGKRLYMTATPRIYSDIAEAKAEKADAKLY
                     GMNNEQWYGKELYVLSFSEAVKQGLLVDYKVLVLAMDESHISSSLQKILKDENNQIKV
                     EDAAKIVGCWKALSKQDTREDLTDDTNPMRRAVAFCQVIEYQTGAKTHKISSKKIASM
                     FKAVVEEYKASNPDDTNTAATLKCEASHIDGGMSASEKDAQLNWLKAEVPDNTCRILS
                     NVRCLSEGVDVPALDAVLFLTPRNSQVDVVQSVGRVMRLAEGKKRGYVVLPVVIPAGM
                     APEEALNDNKTYKVVWQVLQALRSHDDRFDAMVNKLDLIGKDISKMEVIAVSDKIAKK
                     AEKSSTKPHEHGHGTFGIGNTNKDYDLEGQIKEQSILKFEIGEIERAIYAKVVQKCGN
                     RSHWEDWANDIAKIARTHITRITTILADLDNAQERAVFEEFAKELRDDLNDSISDGEI
                     IEMLAQHLITKPVFDALFSNFSFAQHNPISQAMQGVLEVLEKHNLGKEADTLEKFYAS
                     VKMRAEGIESAEGKQKIVVELYDKFFRNAFPKMTERLGIVYTPVEVVDFIIHSINHIL
                     QTEFGQTLGSKGVHIIDPFTGTGTFITRLMQSGLIKSEELPHKYQHEIHANEIVLLAY
                     YIAAINIEAVYHSIVGGDYQPFEGICLTDTFQLYEKEDLVDQILVKNSARRKRQKKLD
                     IRVIMGNPPYSAGQEDENDNNKNIDYPYLDQRISETYIEKSSKKMGKSKSYDSYIRAF
                     RWASDRIGDSGVIGFVTGSGFAEKPAMDGVRKVLHEEFSSIYVLNMRGDIRKNILSKG
                     KAKEGENIFSAGSTTGISITILVKNRSSKSRGEIYWFDIGDDLKTDEKLAILRELAGI
                     SGISSSNKWKIIEPDAHNDWIKQRDENFMNFISLGNKADKGSINLFLTYSMGLKTNRD
                     SWVYGQSRKVLEENIRGMIDFYNSELNRLQGLPEKDVHEEINRDKSKIKWTTDIQSDL
                     VKGRKHDVDFNDFRISLYRPFMKSWVYANAKWNWTRHLMPKYFPNESSENLVICASGV
                     GARAGFSALICNTILDLQVVDNGQCFPLYLYESSEESYDIDFEGTGDLFATKANMHAA
                     GKSGYTRKDAITDSGLKYFQDAYPSEKISKEDIFYYVYGLLHSPDYRESYADNLTKEL
                     PRIPCVKTAQGFWHFSQAGRDLAALHLNYETVEPYPVTLEITGKTPLADEDYRVEKMK
                     YPKKGKDKDLTTLIYNHRITLKGIPLEAYDYIVNGKPALDWVVERQCVKTDKDSGIVN
                     DANDWAIETMGNPKYPLELFMRVITVSLETMKIVNALPKLDI"
     gene            6301..6738
                     /locus_tag="PKZ52_03880"
     CDS             6301..6738
                     /locus_tag="PKZ52_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58941.1"
                     /translation="MKTENDFYISDIKAIERDLYRNGNANVPKFTDKDGNIRPQDAKL
                     KTDSATGIAYVFPSHAGVSAHEIFDGGKNRWKIKAKTAIPKGLALAFDLVTPGHVHIV
                     ATRPMTQTEYIGLLELMFKDAERITNILAELALNKSKLHEKRA"
     gene            6741..6992
                     /locus_tag="PKZ52_03885"
     CDS             6741..6992
                     /locus_tag="PKZ52_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58942.1"
                     /translation="MSENTITVDWSECIVLFNSLLRNIEQLREIVDADGIDDDELYEA
                     EEELNDYVILLAHLKNRYSNLADKGELSPGLKKRLAAVS"
     gene            7194..7412
                     /locus_tag="PKZ52_03890"
     CDS             7194..7412
                     /locus_tag="PKZ52_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58943.1"
                     /translation="MTEEKEDEYFIHKRALGELFDSKFDLNVNLWRGRNPHNSDAIFY
                     PILKAFKLSNGNLRPEDIKTYEKTASFG"
     gene            7654..7899
                     /locus_tag="PKZ52_03895"
     CDS             7654..7899
                     /locus_tag="PKZ52_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58944.1"
                     /translation="MLDLGDLATIIRLIEIELFQLQKKIEGDDLEESDNAAEIIVYTD
                     NLAVKLKNMYEALWDQNSGYPPYPALVADYRKYPPFL"
     gene            7921..8076
                     /locus_tag="PKZ52_03900"
     CDS             7921..8076
                     /locus_tag="PKZ52_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58945.1"
                     /translation="MIYQQIAKEAHDWAIETMGNPKYPLELFMRVITVKPGNNENVNG
                     LPKLEME"
     gene            complement(8089..8352)
                     /locus_tag="PKZ52_03905"
     CDS             complement(8089..8352)
                     /locus_tag="PKZ52_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58946.1"
                     /translation="MLSGLWRLIIEGRLDLSFTVIERHYVRLEKLATGLLQISLSGFH
                     SGAGGDRFDEKIFKGPLARMIREALSYIQLKIKALKVGFILFK"
     gene            complement(8321..10165)
                     /locus_tag="PKZ52_03910"
     CDS             complement(8321..10165)
                     /locus_tag="PKZ52_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNG58947.1"
                     /translation="MAIIVPNINSGSVKMTSGERRFGQRLAQLLEDDYLCWFDVPVGN
                     SRRYPDFIILHPSRGLLFLEIKDWKLSSLHSINHHTAQLLTPQGLITTANPIEQARQS
                     CYQVIDHLKRDPMLRQSSGKHQGNLACPFGYGAVFTHITRKQIQTACMNGGEAVLPMH
                     LIICQDEMLEKTDPEEFQQRLWAMFNYQFSHSLTLPQIDRMRAHLFPEIRIPATNNDL
                     FAPQEQAAQPVTAIPDIIKVMDMQQELLARSFGEGHRVVHGVAGSGKTLILGFRCWYL
                     AQLHSNPILVLCYNISLAAKLRALMRDKAIDHQVQVYHFHDWCGQQLRSYQVDLIKSP
                     QPIWERQVLSVIKGVECGHIPRAQYSAVLIDEGHDFEADWLKLIVQMIDPATNSLLLL
                     YDDAQAIYTQKSRLNFTLSSVGIQARGRTSILKLNYRNTREILHFAFEFAQNYWQPTT
                     ADEDAVPLVKPDAAGNSGLPPVFKLLPVATQELTFISACLRKWHQDGVAWADMAVIYC
                     HHSTGRALLRTLNNDKIPVNWLGSSQSRRSYNPAEDKVVLITRQSSKGLEFPRVIVAG
                     IGALNDDESVQADEARLLYVAMTRAQEYLLLTASRINQYVKRFMAFDN"
     gene            10405..>10756
                     /locus_tag="PKZ52_03915"
     CDS             10405..>10756
                     /locus_tag="PKZ52_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58948.1"
                     /translation="MVIVPRILTLFTLMCLLGLTGCGSGNKSKPDTNTTSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGETKPNTSSSSSGIIVSSSSSSSS
                     SSSSSSSSSSSSSSS"
     assembly_gap    10757..10856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3173 a         2457 c         2537 g         2632 t
ORIGIN      
        1 agcaacaact tgcccatgca gttaaagatg ccaatgggcg cgcctataac cgcactaaac
       61 acctgccgga acggcaagaa atgatgcagg cttgggcgga ttatttggac aaactgaggg
      121 aaacccaatg agcagcaata ccccggtaat acccccaggt tacggtgaat ggctaaccca
      181 gttaaaagcg gatatagccc aatcgcgcaa ccgcgccgcg ctggcggtga atggagaact
      241 aatccaactc tatgggcgca ttggccgcga tattttagag cggcaacagg cccaaggctg
      301 gggggcaaag gttattgatc ggttagccaa tgatttaaaa acagctttcc ctgatatgcg
      361 cggcttttcg agtcgcaacc tcaaatacat ggcattcttc gcgcagcttt gccctaccct
      421 ccaatttggg cagcagcctg ctgcccaatt accctggttc cacatagtca cgctgttaac
      481 aaaagttaac actgaggcgg cgcgggagtg gtacgcacag caagccgtga aatccggctg
      541 gtcgcgcagc acccttgaaa tcaatattaa aaaccaacta cagcaacgcc agggcgccgc
      601 cgtcaccaat tttgccgccc gtatgcctgc aactgattcg gccttggccg ttgaaacatt
      661 aaaagaccca tacctattcg attttttagg cctgggtaat gacgcccatg agcgggaaat
      721 tgaaaacggc ctaattcagc acatcacccg ttttttgtta gagctgggcg caggctttgc
      781 ctttgtaggg cggcaattcc gcttagaggt ggcaggggat gaatttttta ttgatctgct
      841 tttttaccac acgcggctga aggcttacgt agtggtggag ctaaaagcca ctgactttaa
      901 acccgaacac gcgggccaac ttaattttta cctcgccgcg gtggatgcgc aaataaaagc
      961 ggacgacgat aaacccacca ttggcctgct gctgtgcaaa caacaaaacc ggctagtggc
     1021 tgaatatgcg ttgtcaggta ttgataaacc cattggtatt gcccagtacc agttgctgcg
     1081 tgaattgcca gaaatattag tgaaaagcct gcccagtatt gaagaaatag aagcggaatt
     1141 tgcggccagt ttgccagatg attaacaggc aaacacgcca accgttaaac ctctcaaata
     1201 attaaaccaa caaacaatag aatcacggaa cagaacatgt ctgcactgca aaccctgctc
     1261 gataccttcc gcaacaccgc cctaactgag cgggagaaag gcgcctattt tgaaagcctg
     1321 gtaaaggtct atttacttaa cgagccttcc tataaggacc tgtttaacgg caaagtattt
     1381 ttatgggaag agtggcgcaa gtactggatt gctcaaggca atagcgaccc gcaaagtgat
     1441 gccggtatcg acttagtggc agtggaagac gttgccgaaa acccgcgcat attcgccatt
     1501 caagccaagt tttacgcgga agattccaaa atccgcaaag gcgacggcat agactccttt
     1561 ttaagcgcca tgggcaaaaa cccctacacc aatggccttt tgtttatcac taccagcaaa
     1621 gccagcaccc atgtgattga aggaatgaaa aagcgggata agcccgtcaa tatcatttcc
     1681 ctattcgatt tggaaagcag ccaaatagat tggagccaat accaatccac aaaggccccg
     1741 gtattaaaag ccaaaaaatc cctaagggcc caccaatctg aagcactcac caatgtgctc
     1801 gaagggttta aaaccgtaga ccgtggcaaa ttaatcatgg cctgcggtac gggcaaaacc
     1861 tttaccagcc taaaaatcgc ggaggccgtg gcaggcaaag ataagcgggt tttatttttg
     1921 gtgcctagcc tctcgctact gtcacaaacc cttaccgaat ggacacagga aagcaccaca
     1981 cccctgcaca gttttgcggt gtgttccgat agcgatgtgg gtaaaaaacg cggtaaagat
     2041 gaagatgccg tgcaaatgtt aatgcacgaa ttgcgctacc cggccaccac tgagcccgcc
     2101 cgcctggccg ctgaaatgac caaacgccac gatgccgacc acatgagcgt ggtatttagc
     2161 acctaccact ccattgaggt aatcagcaaa gcccagcagc aatttaacct ggcagatttt
     2221 gatttaattg tttgcgatga ggcccaccgc accactggtg ccacttttga tggcgatgat
     2281 gaaagcgcct ttgtaaaagt gcacgacgcc aattttattc gcgccggcaa acgcctttac
     2341 atgacggcca cacctcgcat ttatagcgat atagctgaag cgaaagcaga aaaggcagat
     2401 gcaaaacttt atggaatgaa taatgagcaa tggtatggaa aggagcttta cgtcctttca
     2461 ttttccgaag ctgtaaaaca aggcctatta gtggactata aggtgctggt tttagctatg
     2521 gatgaaagcc atatcagcag cagcctgcaa aaaattctca aagatgaaaa caaccaaatt
     2581 aaggtcgaag acgccgcaaa aatcgtgggt tgctggaagg cgctcagcaa acaagataca
     2641 cgagaagatt taacggatga caccaacccc atgcgccgcg ccgtggcctt ttgccaagtg
     2701 attgaatatc aaaccggtgc caaaactcac aaaatcagtt ccaaaaaaat cgcctctatg
     2761 ttcaaggcgg tagtggagga atacaaagcc agcaacccgg acgacaccaa taccgctgca
     2821 acacttaagt gcgaagcctc ccacatagat ggcggtatga gtgccagcga aaaggacgcc
     2881 cagctcaatt ggctaaaagc cgaagtaccc gataacacct gccgcatttt aagtaatgtg
     2941 cgctgtttat ccgaaggggt agacgtaccg gctttagatg ccgtgttatt ccttaccccg
     3001 cgcaactccc aagtggatgt agtgcaatcg gtagggcgtg taatgcgctt agccgaaggt
     3061 aaaaagcgcg gttatgtggt gctgcctgtg gtgattccgg cgggcatggc gccggaggag
     3121 gcacttaacg acaacaaaac ctacaaggtc gtctggcaag tattacaagc cttgcgctct
     3181 cacgatgacc gctttgatgc catggtgaat aagctggatt taatcggcaa agatatttcc
     3241 aaaatggaag ttattgccgt tagcgataaa attgccaaga aagccgagaa aagttcaaca
     3301 aaaccccacg agcacggcca cggcaccttt ggcattggca acaccaataa agactatgat
     3361 cttgaagggc aaataaaaga acaatccata ttgaagtttg aaattggtga aattgagcga
     3421 gccatttacg ccaaggtggt gcaaaaatgc ggcaaccgca gccactggga agattgggcc
     3481 aatgatatag ccaaaattgc ccgcactcat atcacccgta taaccactat tttggctgac
     3541 cttgataacg cccaggagcg cgccgtattt gaagagttcg ccaaagagct gcgcgatgac
     3601 cttaacgaca gcattagtga tggcgaaatt atcgagatgc tggcccagca tttaattacc
     3661 aagccggtat ttgacgccct ttttagcaac ttcagttttg cccagcacaa ccccatatcc
     3721 caggccatgc agggcgtgtt agaagtgctt gaaaaacaca acctgggcaa agaggctgac
     3781 acactcgaaa aattctatgc cagtgtaaaa atgcgtgctg agggtatcga gagcgccgaa
     3841 ggtaaacaga aaattgtggt ggagctttac gataagtttt ttcgcaatgc cttccccaaa
     3901 atgacggaac gcctaggcat tgtgtataca ccggtagaag tggtggattt tattattcac
     3961 agcatcaatc atattctgca aacggaattt ggccaaaccc tgggcagcaa aggtgtgcat
     4021 attattgacc cttttaccgg caccggcact tttatcaccc gcctaatgca aagtggcctg
     4081 attaaatcag aagaattacc ccataagtac caacacgaaa tccacgccaa tgaaattgta
     4141 ttactggcct attatattgc ggccattaat attgaggcgg tgtaccacag tattgtggga
     4201 ggtgactacc agccgtttga ggggatttgc cttacggata catttcagct gtatgaaaag
     4261 gaagatttgg tagaccagat cctggtgaaa aatagtgcgc gacgtaagcg gcagaagaag
     4321 ctggatattc gggtgatcat gggaaatccg ccgtattcgg ctgggcagga agatgaaaat
     4381 gacaacaaca aaaatataga ttatccgtat ctcgatcaaa gaatttcgga aacttatatc
     4441 gaaaaatcat caaaaaagat gggaaagagc aagagctatg actcatatat aagagcattt
     4501 cgctgggcga gcgaccggat tggtgacagc ggtgtaatag gatttgttac aggcagtggc
     4561 tttgcagaaa agccagcaat ggatggagtg aggaaggtgc ttcatgagga attttccagt
     4621 atatatgtct taaatatgag gggtgatata aggaaaaata tactaagcaa aggcaaagcc
     4681 aaagaggggg aaaacatatt ttccgccggt agcacgactg gaatatcaat aactatttta
     4741 gtgaaaaaca ggtcatcaaa aagcagaggt gaaatatatt ggtttgatat aggtgatgat
     4801 ctcaagactg atgaaaagct ggctattctc cgcgagcttg ctggaatttc aggaatctcc
     4861 agctccaaca aatggaaaat aattgagccg gatgcacata atgactggat taagcagcgc
     4921 gatgaaaatt ttatgaattt tatatcgcta ggaaataaag cagataaagg gtctataaat
     4981 ttatttttga cttattctat ggggcttaag acaaaccggg atagctgggt atatggtcaa
     5041 tcgaggaaag tactggaaga aaacattaga ggtatgatcg atttttacaa ttcagaatta
     5101 aatagattgc agggattgcc agaaaaagat gtccacgaag aaataaatag agataagtcg
     5161 aaaataaaat ggacaacaga tattcaatcc gatctagtaa aggggaggaa acacgatgtg
     5221 gattttaatg acttcaggat ttctctgtat cgtccgttta tgaaatcatg ggtatatgca
     5281 aatgccaagt ggaattggac taggcacttg atgccgaagt acttcccaaa tgaaagttct
     5341 gaaaatttag taatttgcgc tagcggcgtt ggggccagag ccggtttctc agcgcttatc
     5401 tgcaatacta ttcttgatct tcaggttgtc gataatggcc aatgtttccc gctgtacttg
     5461 tacgaatcct ccgaagaaag ctatgacatt gattttgaag gcacaggtga tctgtttgcc
     5521 acgaaagcaa acatgcacgc cgctggcaaa agcggataca cccgcaaaga tgccatcacc
     5581 gatagtggcc taaaatattt tcaagatgct taccctagcg aaaaaatcag taaagaagat
     5641 attttctatt acgtctacgg ccttttgcat tcacccgatt accgtgagag ctacgccgat
     5701 aatttaacca aagagttgcc ccgcatcccc tgcgtaaaaa ccgcccaggg cttttggcat
     5761 ttttcccaag ccggtcgcga tttagccgcc ttgcacctca actatgaaac ggtagagcct
     5821 taccccgtaa ccctcgaaat aaccggcaaa accccactgg ccgacgagga ttaccgggtg
     5881 gaaaaaatga aatacccaaa aaaaggtaaa gacaaagacc tcaccactct aatctacaac
     5941 caccgtatta cccttaaagg aattccgctg gaagcctacg actacatagt caacggcaaa
     6001 cccgccctgg attgggtggt agagcgccaa tgcgtaaaaa ccgacaaaga cagcggcata
     6061 gttaacgacg ccaacgactg ggccatagaa accatgggca accccaaata ccccctggag
     6121 ctgtttatgc gggtaattac cgtaagcctg gaaaccatga aaattgtaaa tgccttaccc
     6181 aaacttgata tttgattgga acatattgtt agataagtac aggtgcttga agcggctgca
     6241 tttggattac gtgctccggt ttgtgcatga gcgaattgaa aaccagcgag gatgaccaca
     6301 atgaaaacag aaaatgattt ttatatctca gacattaaag ctatagagcg cgatttgtac
     6361 cgaaacggca atgccaacgt gcctaaattc acggataaag atggcaatat acggcctcaa
     6421 gatgccaagt taaaaaccga cagcgccacc ggaattgcct acgtgttccc ttcacacgca
     6481 ggggtgtcgg cccatgagat ttttgatggt ggtaaaaacc gctggaaaat aaaagccaaa
     6541 acggccatcc ctaaaggttt ggctttggcg tttgacttgg tgacacctgg ccacgtccac
     6601 attgtggcaa cacggcctat gacacaaaca gaatatattg gcttgcttga gttaatgttc
     6661 aaagacgctg agcgcatcac taacattttg gctgagctgg cattaaacaa aagtaaatta
     6721 cacgaaaaaa gggcgtaacc atgagcgaga atacaattac tgttgattgg tctgaatgta
     6781 ttgtgctgtt taattcctta cttagaaata tcgaacaatt gagagaaatt gttgatgctg
     6841 atggaattga cgacgatgaa ctgtacgagg cagaggaaga gcttaatgat tatgtgattt
     6901 tattggcgca tttaaaaaat cgctattcta acttggctga taaaggtgag ttatcaccgg
     6961 gtttaaaaaa acgattggcc gcagtgagtt agcgggtcag cgactgggcc attgaaacca
     7021 taggcaatgc ccgctacccg ctagagttat ttcagcgcgc aatcacggta agccttgaga
     7081 cgatgaaaat agtgaatgct atgccgattg gatatttgat ctgcgcatca tttaaagctg
     7141 tctagttgct gcatgtgcag cgtttttagt ttgttagcaa aatgaggttg tttatgactg
     7201 aagaaaagga agatgaatat tttatccata agcgcgcact tggcgagttg tttgactcaa
     7261 agttcgacct gaatgtgaat ttatggcgag gccgaaaccc tcacaacagc gatgcaattt
     7321 tttatccgat tctaaaagcg tttaagttgt cgaatgggaa ccttcggccg gaggatatta
     7381 aaacctatga aaaaaccgcg agctttgggt gaaggcgaat agtggcggca tctcgctttt
     7441 tgatcgcagc ggaataccat ccaaaaactg ggagtattat aaattgcttg caggcaccga
     7501 cattccctat ggcttggtta ttactaaaga cgactataag cagaatttta aagctaccca
     7561 ttacaccata agaccaaact gggacatgcc cgtggctaaa ttctgcatat tgctcgataa
     7621 attagcagcc aaactcgtga aggaggttct ctaatgttag acctaggtga tttagcaacc
     7681 attatccgcc ttatcgaaat tgaactgttt caactgcaaa agaaaattga gggggacgac
     7741 ttggaggagt ctgataatgc tgctgaaata attgtttaca ccgacaattt agcggttaaa
     7801 ttgaaaaaca tgtatgaagc gctttgggat cagaattcag gttacccacc atacccagca
     7861 ttggtagctg actatagaaa atatccacct tttctataag ctaaaagcat aaaaacaggg
     7921 ttgatatacc agcagatagc taaagaggcc cacgactggg ccatagaaac catgggcaac
     7981 cccaagtacc cgctggagct gtttatgcgg gtgattaccg ttaagcctgg aaacaatgaa
     8041 aatgtgaatg gcctgccaaa actggagatg gagtaaatga cttactcgtc atttgaagag
     8101 tatgaagccc accttcaaag ccttaatttt tagctgtatg tagctcaagg cctcacgaat
     8161 catccgcgcc agtgggcctt tgaaaatctt ttcatcaaag cggtcaccac ctgcgccact
     8221 atgaaagcct gatagcgaaa tttggagcaa tcccgtagcc aatttctcaa gccttacata
     8281 atgcctttca ataacagtaa aggataaatc cagccgcccc tcaattatca aacgccataa
     8341 accgcttaac atactgattt atgcgggagg cggttaataa taaatattct tgggcgcggg
     8401 tcatggctac gtataacagc ctggcctcat ccgcttgaac gctttcatcg tcattgagcg
     8461 cacctatacc ggccactatt acgcggggga attctaggcc tttactgctt tggcgtgtaa
     8521 ttaaaacaac tttatcttcc gccgggttgt agctgcggcg gctttggctg ctgcccagcc
     8581 aattaaccgg gattttgtcg ttattaaggg tgcgcagtaa tgcgcggcct gtcgagtgat
     8641 ggcagtaaat tacagccata tccgcccagg ccacaccatc ctgatgccat ttgcgtaaac
     8701 aagcgctaat aaaggttaat tcctgtgtag ctacaggcaa taatttaaat actggcggca
     8761 ggcctgaatt acctgcggcg tcgggtttaa ccaggggcac tgcatcctcg tcggcggttg
     8821 ttggctgcca ataattttgc gcgaactcaa aggcaaaatg cagtatttcg cgggtgttgc
     8881 ggtaattgag ttttagaata ctggtacggc cgcgcgcttg aataccgacg ctggataagg
     8941 tgaaattcag gcgggatttc tgggtgtaga tcgcttgagc atcgtcatag agcaacaata
     9001 aagaattggt ggccgggtcg atcatttgca caattaattt taaccagtcc gcttcaaaat
     9061 catggccttc atcaattaat acggcactgt actgcgcgcg aggaatgtgc ccgcactcga
     9121 caccttttat aacgcttaac acttgccgct cccaaatggg ttgcggtgat ttaataagat
     9181 ccacttggta gctgcgcaat tgttggccgc accaatcgtg aaagtggtag acctgcactt
     9241 ggtgatcgat ggctttatca cgcatgaggg cgcgcagttt tgccgctagg gaaatgttgt
     9301 aacacaagac taatatgggg ttggaatgga gttgtgccaa ataccaacag cgaaagccta
     9361 aaattagggt tttacctgag cctgccaccc cgtgtaccac cctatggcct tcgccaaaac
     9421 tcctggccaa taactcctgt tgcatatcca taactttaat gatgtccggt atggccgtta
     9481 caggttgggc ggcctgttcc tgtggtgcga ataaatcgtt gtttgttgca gggatacgaa
     9541 tttccgggaa tagatgtgcg cgcatacgat ctatttgcgg cagcgtcaaa ctgtgggaaa
     9601 actgataatt aaacatggcc caaaggcgtt gctgaaattc ctctggatca gttttttcca
     9661 gcatttcatc ctgacagata attaaatgca tgggcaaaac tgcttcaccg ccgttcatgc
     9721 aagccgtttg tatttgtttg cgcgtaatat gggtgaacac ggcgccataa ccaaacgggc
     9781 aggctaagtt gccttggtgt ttaccacttg attgccgcaa cataggatcg cgttttaagt
     9841 gatcaatgac ctgataacag ctctgccgcg cctgttctat aggattagct gtggtaatta
     9901 aaccctgcgg cgtgagcaat tgtgctgtgt gatggttgat agagtgtagg ctggagagct
     9961 tccaatcttt tatttctaaa aataacaggc cccggctcgg gtgcaaaata ataaaatccg
    10021 ggtagcggcg gctattgccc accggcacgt caaaccaaca cagataatca tcttccaata
    10081 attgcgccaa gcgctgacca aaacgcctct cgccgctggt catttttacg ctgccagaat
    10141 ttatattggg gacgataata gccatttatg taccgagcgc tgcttgttgg gcacattaaa
    10201 tttaacccga caattgtgat gagaggccgt tgattattgc acaaaagcat gctgaatcgc
    10261 cgctcattaa tgatgcacaa ttagccgccg ctcgactcct atttacacca ggcgggcggc
    10321 gaaacttcgg cttaaacatg caggtttagc ttattgcaga atcactttat actgccgctt
    10381 ttcaatcaat atggaaggtt acttatggtt attgtgccta gaatacttac gctcttcaca
    10441 ctcatgtgcc tgctgggttt aaccggctgt ggcagtggca acaaatccaa accggatacc
    10501 aacactacaa gctccagcag cagctcctct tcaagctctt catcctccag ctcttcctcc
    10561 agcagctctt cctccagcag ctcttcctcc agcagctctt cctccagtag ctcttcctcc
    10621 agtagctcaa gttcatcagg cgaaaccaaa cccaatacct caagctcgtc atcggggata
    10681 attgtttcta gcagttcttc aagttcttca agttcttcaa gttcttcaag ttcttcaagt
    10741 tcttcaagtt cttcaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcag
    10861 ctcgtctagc tcaggtgctc tggtaaaaac tgaggtgag
//
LOCUS       DAOQGS010000066        10829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2833_length_10829_cov_4.880546, whole genome shotgun sequence.
ACCESSION   DAOQGS010000066 DAOQGS010000000
VERSION     DAOQGS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2833_length_10829_cov_4.880546"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKZ52_03920"
     CDS             <1..602
                     /locus_tag="PKZ52_03920"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG58949.1"
                     /translation="KAEMKEWIEHNLKLVHMDHAMHKRPGEISGGMKQRVGIARALAM
                     EPKVLLLDEPFGALDALTRAHMQDSLMEIQRDLKNTVIMITHDVDEAVLLSDRIVMMT
                     NGPAARVGEILEVDLPRPRDRLALAEDPHYNHLRQEVLRFLYEKQRKTEDGLAAPSAA
                     AKSDSKKNSSELVPEIKKIPTAEPDKKVINLHTHKDHVA"
     gene            768..2057
                     /locus_tag="PKZ52_03925"
     CDS             768..2057
                     /locus_tag="PKZ52_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNG58950.1"
                     /translation="MSSFPFPKSLLCALITCAPMTTYAGEDAFYDALSGGKASGNFRL
                     RYESVDVTSPTVKDAEALTLRSRLGYETAPLAGFTAMAEFEDTRIIGAFDEYAPNKAG
                     FAAIVDPEQTELNQAFVRYRGISKLDVIAGRQKILLDNQRFVGNVGWRQNEQTFDALT
                     LNYKGISDWSFYYAYIGAVQGIAKIKPNTNFSFDTDDHLLNIQYSGFQLGKITAYAYL
                     LNNQALDLNQLNPALRYQANDTLGLRFDGAYVMPTTLPLRLLYTAEYAQQTLTNPALV
                     EKDMDYYYLDLGIAYTTKQGPLVFKVYEESLGSDKGTQGFQTPYATKHAFEGWADMFL
                     GTPTTGVVYDTATLSMDMTALGIKWLINYGTYKQDEGNKDFGDEINAQVLKAFGKKYT
                     IGLKYADYSADKAIATINPVTPNIDTKKVWLWGEMAF"
     gene            complement(2253..3323)
                     /locus_tag="PKZ52_03930"
     CDS             complement(2253..3323)
                     /locus_tag="PKZ52_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58951.1"
                     /translation="MTTTTEEITLKTATASRRKIVRIISTTAVAMPVLNLLGCGGDTS
                     SGSSSSSSSSSSSSSSSSSSSGRSSSSSSSSSSSSSSSSSSSSSSSSSSSTSSSGGGA
                     ITAANLAKGGTKNIKIPFPPENPFKNGIFSTPLTCSKPTGTQILGPCYLDLGQFREDI
                     SDGEAGVPIIFAFQVLDKNCKPMPGLAVHIWHTNCKGQYSADNTKGTPGAGWTPKVCS
                     GETPLTSTTTFPASEVQRAYKSTWHRGATSTDMEGVAYFKSCFPGWYGGRTNHVHFRF
                     VQNNKELLVTQLGFSDALCDEIHKNHPDYIGKIQDRTMLSISGRQQDTTFSGEPKTQW
                     IMDAYRNSDGSLTISKTIILNV"
     gene            complement(3770..6163)
                     /locus_tag="PKZ52_03935"
     CDS             complement(3770..6163)
                     /locus_tag="PKZ52_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58952.1"
                     /translation="MIINLKKQSLAYGGVVLLALGLTACAGPGESSSSSSSSSSSSSS
                     SSSSSSSSSSGRSSSSSSSSGRSSSSSSSSSSSSSGKPGNGPKVGYSVLDNGNGGVNY
                     EPVDLVNLYEDTGYDRAKSIEAFKATLYPELKKFGCGACHNSAIAQEYGGQQAPMHTD
                     TNPELAHDYALTRVNFKDPENSRFVVRLKLERHNCPPSLGTCADGAKAMLTAINAWKD
                     KVKDMLPETPRGVPASTKISEAEMKQWIAEDKAKIPAADQKYIVYTSLHELHNEGLTA
                     DQLNVVRVGLSKALNSTARWAPAVVNPTDVNGKGILYKFDIRVYWGYNQGVKELLFGG
                     SDDDLAFGNGKKDYKGTAVTASVQNKKYNFTGKTVKDPEHALRIWERILHGNVEGAVT
                     SGTIPPYIDGFKGTRKTNAAGEYVDINGFEWVEAAQLTYTITRPDVYNAIMANPMFAD
                     EMESHLGMDNSKGMDSYDYMLVKDAITVDSRLLFRANRKGDQATDPRTGNKSFYWKSF
                     DVFSGQLASGRDRSIFDVYDDPTGKDIRFPWWANPIPKFVLWESVADDTKYFSHVASL
                     NQANQGGQLANFIDAATAGCDPQTSFAGGNFRNCRHYTGKGGFMSSAAEIIYNLPNGL
                     NGYYLLGGHNQRRVDAFINIVRDPRIIIDAKDDIASQTGFEFSKNSGRGGANDPRLNV
                     GSSCIGCHVDGMNRMNNDIRLWLDKAPDLLPKGPYGADKWKTDAATVKRVRELYKENS
                     VMKAQVEEDRKIFLEAMADIKHEMVWGENKNVYVEPVIWTVEYVQRVKYKYKQTTSN"
     gene            7262..7564
                     /gene="ureA"
                     /locus_tag="PKZ52_03940"
     CDS             7262..7564
                     /gene="ureA"
                     /locus_tag="PKZ52_03940"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNG58953.1"
                     /translation="MELSPRDKDKLLIFTAALLAERRKARGLKLNYPESVAFISAAIM
                     EGARDGQTVAQLMSYGKTLLTREDVMEGVAELILEVQVEATFPDGTKLVTVHEPIV"
     gene            7621..7929
                     /locus_tag="PKZ52_03945"
     CDS             7621..7929
                     /locus_tag="PKZ52_03945"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNG58954.1"
                     /translation="MIPGEYQIAPGAIALNTGRPCVRVSVANTGDRPIQVGSHYHFYE
                     VNPALVFDRPTTLGFRLNIAAGTAVRFEPGQSREVELVALAGLRKVYGFRGDVMGDLP
                     "
     gene            7926..9623
                     /gene="ureC"
                     /locus_tag="PKZ52_03950"
     CDS             7926..9623
                     /gene="ureC"
                     /locus_tag="PKZ52_03950"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNG58955.1"
                     /translation="MSTMSRAAYADMFGPTVGDRVRLGDTDLWIEVEQDFAIYGEEVK
                     FGGGKVIRDGMGQSQASCADTPDTVITNALILDHWGIVKADVAIKNGRISGIGKAGNP
                     DIQPGVTINIGPGTEVIAGEGQILTAGAIDAHIHFICPQQIEEALMSGVTTMIGGGTG
                     PATGTNATTCTPGPWHMARMLQSLEAFPMNFGLLGKGNASLPTALHEQIAAGAMGLKL
                     HEDWGTTPASIDTCLTVAEECDIQVAIHTDTLNESGFVEDTIAAFKGRTIHTYHTEGA
                     GGGHAPDIIKACGLNNVLPSSTNPTRPYTINTVDEHLDMLMVCHHLDPSIPEDVAFAD
                     SRIRKETIAAEDILHDLGAFSMISSDSQAMGRVGEVIIRTWQTAHKMRAQRGSLAGDV
                     ADNLRAKRYIAKYTINPALAHGISHEVGSVEVGKLADLVLWKPAFFAVKPSLIIKGGM
                     IAAAPMGDANASIPTPQPVHYRPMFGAYGRACRATSVSFVSQAALDAGIAGSFELERR
                     LVAVKGCRSVNKAQMVHNSYQPVMEVDPQSYEVRADGVLLTCEPADVLPLAQRYFLF"
     gene            complement(9791..10183)
                     /locus_tag="PKZ52_03955"
     CDS             complement(9791..10183)
                     /locus_tag="PKZ52_03955"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNG58956.1"
                     /translation="MNPIDETPTPAGELALQTLVLPKDTNSNGDVFGGWLMAQMDLAG
                     AIGAREVAKGRVTTVAVGSMTFLRPVPVGATVSCYCDVLEVGRSSIKTRVEVWVKHYD
                     SERQKVTEGEFVYVAIDNAGRTRPVPRG"
     gene            10461..>10829
                     /locus_tag="PKZ52_03960"
     CDS             10461..>10829
                     /locus_tag="PKZ52_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNG58957.1"
                     /translation="MKKLLTALACASSFTLLAPAALAARDNISAVGSSTVFPFAKVVA
                     ERFGRKGNFKAPKIEPTGTGGGFKEFCKGIGVEFPDIANASRRIKQSEYDACQAAGVK
                     EIVEIKIGFDGIVLANSKKAP"
BASE COUNT         2567 a         2875 c         2870 g         2517 t
ORIGIN      
        1 ccaaggctga aatgaaagaa tggattgagc acaatttaaa actggtgcat atggatcacg
       61 ccatgcacaa acgccccggc gaaatttccg gcggcatgaa acagcgcgta ggcatcgccc
      121 gcgcgctggc catggaaccc aaagtattat tgctcgatga acccttcggc gccttggatg
      181 ccctcacccg cgcacacatg caagattctt taatggaaat tcaacgggat ttaaaaaata
      241 ccgtgattat gattacccac gatgtggacg aagccgtatt gctgtcggac cgcatcgtca
      301 tgatgaccaa tggcccggcc gcgcgagtag gtgaaatcct cgaagtggat ttaccgcgcc
      361 cccgcgatcg cttagccctg gctgaagacc cccactacaa ccatttacgc caagaagtat
      421 tgcgcttcct ctatgaaaaa cagcgcaaaa cggaggacgg cttagcggct cccagcgctg
      481 cggccaaatc ggattccaaa aaaaactcta gcgagctagt gcccgagatt aaaaaaatcc
      541 ccaccgccga gccagacaaa aaagtcatta atttacacac ccataaggat cacgtggcgt
      601 aacacccaca caccccaata ttgctgcggc cttggccgca ggcacaaaaa tagtaactag
      661 cggctgccct gatggcagcg cgcacggtgc tttgcgccct taaaacctgg cttttagcca
      721 cggaataaaa attaattcat caccttatga tttatgcgga gtgagcaatg agcagttttc
      781 cttttcctaa aagccttttg tgcgcattaa taacctgtgc ccccatgaca acctatgccg
      841 gtgaagatgc attttacgat gccttaagcg gcggcaaagc ctcgggtaat ttccgcctgc
      901 gctatgaaag tgtggatgtc accagcccca ctgttaaaga cgccgaggcc ctcacgctgc
      961 gcagccgttt gggttatgag acagcgccgc tggcgggctt tacggccatg gcagaatttg
     1021 aagacacgcg cattattggc gcttttgatg aatacgcgcc gaataaagcc ggttttgccg
     1081 ccatagtaga cccggagcaa accgagctta accaggcgtt tgtgcgttat cgcggtattt
     1141 ctaaattaga tgtgatcgcg ggccgccaaa agattttgct ggataaccag cgttttgtgg
     1201 gcaacgtcgg ctggcggcaa aacgaacaaa cctttgatgc gcttaccctt aattacaaag
     1261 gcatcagcga ttggagtttt tattatgcct atatcggcgc tgtgcagggc atcgccaaaa
     1321 taaaaccgaa taccaatttc agcttcgaca ccgacgatca cctgctgaat attcaataca
     1381 gcggttttca acttggtaaa atcaccgcct atgcctattt attaaataac caagcgctgg
     1441 atttaaacca gctcaacccg gccctgcgct accaagcaaa cgacacccta ggtttgcgct
     1501 ttgacggcgc ttatgtaatg cccaccaccc tgccgttgcg tttgttatac accgccgaat
     1561 acgcccaaca aacccttact aatccagctt tagttgaaaa agatatggat tattattatt
     1621 tggatttggg catagcctac accaccaaac aaggcccttt agtatttaaa gtctatgaag
     1681 agagcctagg ctcggataag ggcacccaag ggtttcaaac cccctacgcc accaaacacg
     1741 cctttgaagg ctgggccgat atgttcttag gcacccccac cactggcgtg gtgtacgaca
     1801 cagcaacgct ttccatggat atgacggcgc tgggcattaa atggctgatt aattacggca
     1861 cctacaaaca ggatgaaggc aacaaagatt tcggtgacga aattaatgcc caggtgttaa
     1921 aagcctttgg taaaaaatac acaatcggcc ttaaatacgc cgattattct gcggataaag
     1981 ccatagcaac aattaatccg gtcaccccga atatcgatac caaaaaagta tggctttggg
     2041 gcgaaatggc gttttagcct aacaacctcg gttgtacccc cagcagtaag gatgctccgc
     2101 tatcaatgag tgagatgaca gagttaattg cccatgcctc agttgcacag gttcaactga
     2161 ggttttttat gcgtaaaaaa agcgaagcac ttgtacggag cttcgctttg aattttgtac
     2221 cccaggctga gaatctcagc ccaaagtttc aattaaacat tgagaataat agtttttgaa
     2281 atggttaagg agccgtcaga atttcggtag gcatccataa tccattgagt tttaggctct
     2341 ccggagaagg tggtgtcctg ttggcggccg ctgatgctga gcatggtgcg gtcctggatt
     2401 ttgccaatgt aatccgggtg atttttatga atttcatcgc aaagagcatc agagaagcct
     2461 aattgggtta ccagcaattc cttattgttc tgtacaaaac ggaagtgcac gtgattagtg
     2521 cggccgccgt accaacctgg gaaacaggac ttaaagtagg ctaccccttc catatccgtt
     2581 gaagtggcac cacggtgcca ggtagattta taggcgcgct gcacttcgct ggcggggaag
     2641 gtagtggtag aggttaaagg tgtttcaccg gaacacactt ttggcgtcca accggcgccg
     2701 ggagttcctt tggtgttatc cgccgaatac tggcctttac agttggtgtg ccaaatatgg
     2761 accgccaaac cgggcatggg cttacagttt ttatccagta cttggaacgc aaaaatgatg
     2821 ggtacgcccg cttcgccatc ggaaatatct tcgcgaaatt gccccaggtc taaataacaa
     2881 ggccccaaaa tttgcgtgcc ggtgggttta gaacaggtca gcggtgtaga aaaaataccg
     2941 tttttaaagg ggttttcggg cgggaaggga attttgatat ttttagtgcc gcccttggcc
     3001 aaattggccg ccgtaatagc gccgccgccg gagctggagg tgctgctgga gcttgagctg
     3061 ctggaagacg aactagagct gctggaggat gagctggagc tactggacga cgaactggag
     3121 ctgcggccac ttgagctgct cgaactggag ctggagcttg aactgctact ggagctagaa
     3181 cttgagccgc tggaggtgtc gccgccgcag ccgagcaggt tgagcaccgg catggcaacg
     3241 gcggtggtgc tgataatgcg gacaattttg cggcgggagg ccgtggcggt tttaagggtg
     3301 atttcttcgg tagtggtggt cattgtatgt accctatgca ggcagatgtg ttctgcggct
     3361 aattattgtg agaattactc ggcgaaccca acaacacggg gcaaatacgg gattgccagc
     3421 tgctaatgat atttggcagc acgttgatgt tcggtaccgt aagcgtgatc aacagctcct
     3481 atggtcacag gcacgttacc ataacgctct gcggcaaaag gcgtatgcac gcacaggtaa
     3541 ataggcacct gattcgcatt ttgtattatt tataactcaa atcccattca aatccctagg
     3601 ctggggtcaa tttaggtcat agtgttaaac ctgaacatta acgcgatgct gcaagattct
     3661 cttctggacg ggcgttcgtt acctcgttca taagggcaga aaaaaggggc atgcctcaac
     3721 cagcgccgcc cctatcatct atttagtgtt tcgcacctgt tcggcgaacc tagttagatg
     3781 tggtttgctt gtacttgtat ttaacccgct gcacgtattc aacggtccaa ataacgggct
     3841 ctacgtagac gtttttgttc tcgccccaaa ccatctcgtg cttaatgtcc gccatggctt
     3901 cgaggaatat tttgcggtcc tcttccactt gagccttcat cacggagttt tctttgtaga
     3961 gttcgcgcac gcgtttaaca gtggccgcat cggttttcca cttatccgcc ccataggggc
     4021 ctttgggcaa taaatcgggc gctttatcaa gccacaggcg aatatcgttg ttcatgcggt
     4081 tcataccatc tacgtggcag ccgatacagg agctgcccac gttcaagcgc gggtcattgg
     4141 cgccgccccg gccgctgttt ttgctgaact caaaaccggt ttgcgaggcg atatcgtcct
     4201 tggcatcaat aatgatgcgc gggtcgcgca ctatattaat gaaggcatcc acccggcgtt
     4261 ggttgtggcc gcccaacagg taataaccat ttaaaccatt gggcaggttg taaatgattt
     4321 ccgctgcgga actcataaaa ccgcccttgc cggtgtaatg gcggcagttt ctaaagtttc
     4381 cgccagcaaa gctggtttga gggtcgcaac cggcggtggc cgcgtcgata aagttcgcca
     4441 gctggccacc ttggtttgcc tggttgaggc tcgctacgtg gctgaagtat ttagtgtcat
     4501 cggcaacgct ttcccacaac acaaacttag gaatgggatt ggcccaccag ggaaagcgaa
     4561 tatctttacc ggtagggtcg tcgtacacat caaaaatgct acggtcgcgg ccactggcta
     4621 gctggccgga gaacacatca aaagacttcc agtagaagct cttattgccg gtgcgcgggt
     4681 cagtggcttg atcgcctttg cggttggcgc ggaataacag gcgagaatcc acggtaatgg
     4741 cgtctttcac cagcatgtaa tcgtaggagt ccatgccctt gctgttatcc atgcccagat
     4801 gggattccat ttcgtcggcg aacatagggt tggccatgat ggcgttgtac acatcggggc
     4861 gggtgatggt gtaggtcagc tgggccgctt ctacccattc aaagccattg atgtccacat
     4921 actcccccgc cgcgttggtt ttgcgggtgc ctttaaagcc gtcgatatag ggtggaatgg
     4981 tgccagaggt gacggcgcct tcgacgttac cgtgcagaat acgctcccaa atgcgcagtg
     5041 cgtgctcggg gtctttaacg gttttaccgg tgaaattgta ttttttgttt tgcacagagg
     5101 cggtaacggc ggtgcctttg tagtcttttt tgccgttgcc aaaggccaag tcatcgtcgg
     5161 agccgccaaa taacagctct tttacgccct ggttgtaacc ccagtaaacg cggatatcaa
     5221 acttatagag gatacctttg ccgtttacgt cagtggggtt tacaaccgct ggcgcccagc
     5281 gggcggtgga gttgagggct ttggagagcc ccacgcgcac cacattgagc tgatcagccg
     5341 ttaagccttc gttatgcagc tcgtgcaggg aggtgtagac gatgtacttt tggtccgctg
     5401 cggggatttt ggctttgtcc tcggcgatcc actgtttcat ttccgcttcg gaaattttcg
     5461 tgctggccgg cacgccgcgg ggtgtttccg gcagcatgtc tttcaccttg tctttccagg
     5521 catttatggc ggtcaacatg gctttggcgc cgtcggcaca ggtgcccaat gagggtgggc
     5581 agttgtggcg ctccaatttt aagcgcacca caaaacggga gttttcaggg tctttaaaat
     5641 taacccgcgt gagggcgtaa tcgtgggcca actcggggtt ggtatcggtg tgcatgggcg
     5701 cttgctggcc gccgtattcc tgcgcaatag cggagttatg gcaggcgcca cagccgaatt
     5761 ttttcagttc agggtagagg gtagccttaa aagcttcgat ggatttggcg cggtcgtagc
     5821 cggtgtcttc ataaaggttg accaggtcca ccggctcata gttaacgccg ccattgccgt
     5881 tatccaacac tgagtaaccc accttcgggc cattgccggg cttaccggaa ctgctgctgg
     5941 atgaactgga ggacgaactg gaggagcgcc cgctgctgga tgaagagctg gaggagcgac
     6001 cggaagagct ggaggatgaa ctggagctgg aggacgagct ggaactggag cttgagctgc
     6061 tggaactact ggattctcca ggtcctgcgc atgcggtaag gcccaatgca agcaaaacaa
     6121 ctccgccata ggcaagcgat tgctttttga ggttaataat catttttttg ccctgttgct
     6181 gatttatttt ttagtgatta gcgatgcttg aaaacgcgct ggatgtgcaa ccaaaaatta
     6241 cctgcacgca ccacgcttaa gcggctttgg caaactacca cctttaaatt cacccaaagg
     6301 cgttgatgct cacagtttaa ttggtaatag atcataattt tcagctttat aatacaaata
     6361 attttccagc cgaaaattac ccgccaaaaa agcaaaaaac acggcaaaaa tagctattta
     6421 ttgttttatc gtcatacctt aaaaacaccc caaaatacgg gcgtaatttt tcgcagcctt
     6481 atgcaaaaac gctctactta aaatttacgc ctgcgcattt tgcaacccaa tagtcttacc
     6541 atttgaattt ttattgcgac tgccaagctt gcgtaaataa aaattcaagc gcccgcgcaa
     6601 gtgaaaatat tcagcatctt tttaatagcg ctgaacttaa ttgccgcact ggctgaaact
     6661 atttggcgag tgtttgagtg ctgccaaccg cccgcgtaga gaggctaatt agctgtacta
     6721 aatagttaca agtcactttt ggtgtctgct gaattactac ctagccacca gcaatgaata
     6781 cacatcattt cagaccgtca aaaagaggaa cctttttgac gggcctacac ggatgtattc
     6841 acggcctgtc tgaaatgatg tgtattcatt gcttccgtac agctagtagt tgagcctgat
     6901 ggggatattt agaaatagcg cgttaatttc ttgcctgcat aagcgttcaa cagacctaaa
     6961 gcgagcctaa aacccaccgc cggaagacgt actcttggca cgcccagatg gggtgagcgg
     7021 gcggcctttg aggggtgagt tcaaagcctg tgttagtagc gaacctaggg gcctaaggca
     7081 caagttctgc atattgtggt ggggtggtca gatggtgcac tctatgcctg caggctgagt
     7141 ctgcgcgggc atgccttgcc aaaagaaggc tcaatgttgc gcaagccctg ctgacttgcg
     7201 ccctgcaatg gcccacagtt cctaattttt taggtgtttt tatttcatag gtattttgcg
     7261 catggaactc tccccccgcg acaaagataa attgctgatt ttcaccgccg ctctgctggc
     7321 cgagcgccgc aaagcgcggg gcttaaagct taattacccg gaatcggtgg cctttatcag
     7381 cgccgccatt atggaaggcg cccgcgacgg ccaaaccgtg gcccagctga tgagttacgg
     7441 taaaacactc ttaacgcggg aggatgtgat ggaaggagta gccgaactga ttctagaagt
     7501 acaggtggaa gccaccttcc ccgatggcac aaaactggtg accgtacacg aaccgattgt
     7561 ttaactccct ccattcatgc cgcccttgca gctctagcgg ctaaaaataa aaggtgccct
     7621 atgattcccg gcgaatacca aattgccccc ggcgcgattg cgcttaatac cggccgcccc
     7681 tgtgtgcggg tgagtgttgc caacaccggg gatcgcccca tccaggtggg ctcccactac
     7741 catttttacg aggtgaatcc cgccttagtc ttcgaccgcc ccaccacgtt gggctttcgc
     7801 ttaaatattg cggcgggcac agcggtgcgc tttgagccgg gccagagccg cgaggtggaa
     7861 ttggtggcac tggcggggtt gcgcaaagtg tatggctttc gcggcgacgt gatgggggat
     7921 ctgccatgag cactatgagc cgcgccgcct atgcggatat gtttggcccc acggtggggg
     7981 atcgggtgcg tttgggcgat accgatttgt ggattgaagt ggagcaggac ttcgccatct
     8041 acggcgagga agtgaaattc ggcggcggca aggtgattcg cgatggcatg ggccaaagcc
     8101 aagccagctg cgccgacaca ccggataccg tcatcaccaa cgccctgatc ctcgatcact
     8161 ggggcatagt caaggccgat gtggccatca aaaatggtcg catcagcggc ataggcaaag
     8221 cgggcaaccc ggatattcag ccgggcgtca ccatcaacat agggccgggc actgaggtga
     8281 tcgcaggcga agggcaaatc ctcaccgcag gcgccataga cgcccacatc cattttattt
     8341 gcccccagca gatcgaagag gcccttatga gcggggtcac cactatgatc ggcggcggca
     8401 ccgggcctgc caccggcacc aacgccacca cctgcacccc cggcccctgg cacatggccc
     8461 gtatgctcca aagcctagag gccttcccca tgaacttcgg cctgttgggc aagggcaacg
     8521 ccagcctgcc cactgccctc catgaacaaa tcgctgcggg cgccatgggg cttaaattac
     8581 acgaagactg gggcaccacc ccggcgtcca ttgatacttg ccttacagtg gcagaggagt
     8641 gtgatatcca agtggccatc cacacggata ccctcaatga gtccggcttt gtggaggata
     8701 ccatcgccgc ctttaaaggc cgcactattc acacctacca caccgaaggc gccggcggcg
     8761 gccacgcgcc ggacatcatc aaagcctgcg gccttaacaa cgtattaccc agctccacca
     8821 accccacccg cccctacacc attaatacag tggatgagca cctggatatg ctgatggtgt
     8881 gccaccacct ggacccgtcc atccccgaag acgtggcctt tgccgattcg cgcattcgca
     8941 aagaaaccat cgccgccgaa gacatactgc acgacttagg cgccttttct atgatcagct
     9001 ccgactccca ggccatgggc cgagtgggtg aggtgattat tcgcacctgg caaaccgccc
     9061 ataagatgcg cgcacagcgc ggcagcctgg ccggcgacgt ggcggacaac ctgcgggcca
     9121 aacgctacat cgccaaatac accatcaacc cagccctagc ccacggcatc agccatgagg
     9181 tgggctcggt ggaggtgggc aaattggcgg atttggtgct gtggaaaccg gctttttttg
     9241 ccgttaaacc cagtttaatt attaaaggcg gcatgattgc ggcggcgccc atgggggatg
     9301 ccaacgccag tattcccacc ccgcaaccgg tgcactaccg gcccatgttc ggcgcgtacg
     9361 gcagggcctg ccgggcaacc tcggtgagtt ttgtcagcca ggcggctttg gatgcgggca
     9421 tagcaggcag ctttgaactg gagcggcggt tggtggcggt gaagggctgc cggagcgtga
     9481 ataaggcgca gatggtgcac aacagctatc agccggtgat ggaagtggac ccgcagtcct
     9541 acgaagtgcg cgccgacggc gtactgctca cctgcgagcc tgccgatgta ttgcctttgg
     9601 cccagcggta ttttttgttt taacaggtgc tgggggcaga aactcgggga gcctagcgta
     9661 atcgggtata ggttattgct gtcggggcgc cctgtatgtg attcactgag gattagagtt
     9721 caaggcgaaa cgggcaccca taagaggtgc ccctacagcc attggtcacg cggacttaac
     9781 acgcccgccg ctaacctctc ggcaccggcc gggtgcggcc agcgttgtcg atggccacat
     9841 acacaaactc accttccgtg accttttgcc gctcgctgtc atagtgtttc acccacacct
     9901 ccacccgggt tttaatggag gagcggccca cctccagcac atcgcaataa cagctcaccg
     9961 tggcgcccac cggcaccggc cgcagaaagg tcatgctgcc caccgccacg gtggtaaccc
    10021 ggcccttggc tacttcccgc gcgccaatgg ctccggctaa atccatctgc gccatcaacc
    10081 agccgccaaa tacatcgcca ttggaattgg tatctttcgg caacaccagc gtttgcaagg
    10141 ctaattcacc agccggggtc ggggtttcgt caatgggatt catagagggc tccaggtgtg
    10201 tagcgacggg gagtggcaag agcaacacac ctcactatag cgcgatatgc tgcaccgcaa
    10261 aagccaagat acctgtgatt gatacccaaa cttgacccct ttcaaaaacc caatgcctac
    10321 aacctcgcct gggtcacaag cgagccttgc ccagggcggc ttcatgaata tgcaacaaaa
    10381 ctgtcataat caccccctag catgtgcaac agggcgctca agcgccttct gttaaccacc
    10441 tatccttgag gggatttttt gtgaagaaac tgctgaccgc tcttgcctgt gcttcaagct
    10501 ttactctgct cgcgcccgcc gccctggccg cccgcgacaa tatttccgcc gtgggctcct
    10561 ccactgtatt cccatttgcc aaggttgtag ccgagcgttt tggccgtaaa ggcaacttta
    10621 aagcccccaa aattgaaccc accggcaccg gtggcggatt taaagaattc tgtaaaggta
    10681 tcggcgttga attccccgat atcgccaatg cctcgcgccg catcaaacaa tccgagtacg
    10741 acgcttgcca agcagcgggt gttaaagaaa ttgtggaaat caaaatcggc tttgacggca
    10801 tagtgctggc caacagcaaa aaagccccc
//
LOCUS       DAOQGS010000067        10776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2845_length_10776_cov_4.164817, whole genome shotgun sequence.
ACCESSION   DAOQGS010000067 DAOQGS010000000
VERSION     DAOQGS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_10776_cov_4.164817"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKZ52_03965"
     CDS             <1..735
                     /locus_tag="PKZ52_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNG58958.1"
                     /translation="EANLADLIAKVKEENADLGVAFDGDGDRLVVVTSTGQIIWPDRL
                     LMLFAKDILARNPGADVVFDVKSSRLLTSVVASQGGRPIMWKTGHSPMKAKVQETGAL
                     LGGEYSGHIFIKDRWYGFDDGMYAAARLIEIMSLREQSLDDIFAEFLPLPSTSEYRVK
                     VAEEEKISIVQRLIEEGDFGDAKLITLDGVRVEYPYGWGIVRPSNTESCLTLRFEAET
                     EADIHKLKALFTRELRKIDNRISFAW"
     gene            786..1682
                     /gene="argB"
                     /locus_tag="PKZ52_03970"
     CDS             786..1682
                     /gene="argB"
                     /locus_tag="PKZ52_03970"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNG58959.1"
                     /translation="MTAPKDASQIARVLTEALPYIQRFTGKTIVVKYGGNAMDGAEME
                     NSFARDIVLMKQVGMNPIVVHGGGPQIGELLTKLNIQSHFVDGMRVTDSATMDVVEMV
                     LGGSVNKHIVNLINRNGGQAIGVTGKDGNLINARKMEVTRYCPELKAPEIIDIGQVGE
                     VRSINRKVIDLLVQSDFIPVIAPIGVGSDGTSYNINADLVAGKVAEVLQAEKLMLLTN
                     VSGLLDKQGQVLTGLTTARVDELISDGTIYGGMLPKIQCALDAVKNGVHSAHIVDGRV
                     AHAVLLEIFTDAGVGTLITNRH"
     gene            complement(1830..6554)
                     /locus_tag="PKZ52_03975"
     CDS             complement(1830..6554)
                     /locus_tag="PKZ52_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG58960.1"
                     /translation="MNRLPYVPYPLKKLTCALATISIIPTLWAAPEQGQIIGGQGSIE
                     LQGKNTLIQQASERLSINWQNFDIKSDERVQFIQPAESSIAFNRILSNKGSLIQGRID
                     ANGQVVLINPNGIIFTETASVNVGALVASGLDINNEEFLNSQFTLNALEGASGQVINS
                     GLLNAATGGSINLLGQRVENSGLISANLGSVHLAAGKEAVLTFERDGLIGVRVSEAVL
                     QNDLGVNAAVINKGDITAQGGKVLLTASVSKNIFSQAVNVGELNQAKSAVVNADGSFS
                     LGAGADVENTGAINVGAGAAVVLGENITHSGTINADNTLGDAGSIELNATKTTLNTGA
                     SSLSARANKQGQGGDIKMLGQQVGLLDEAQVDASGAEGGGQILIGGDKTGANPLINNA
                     EFTHISEQAQVRTDALHTGSGGKLIVFAEDSAAIYGHLSARGGDHGDGGFIETSGLHG
                     LEVWNSPDISAPAGVAGHWLIDPYNIEIVNSAVLRPDRISTSTNATQTQSLFTSRNDA
                     AQIGWNSIRNVLDNGSGGTVEIKTSGGSNAGGTIKVAANFDFSTLRSSAPQSGFNASL
                     ILTADKNIEFTDSVTYLRASTNNRLNMEFNAQSGINFNRANLATNGGTLSARTQNGSI
                     TSKATLSTARSGETNNNSGKIQLNAATGSIVLNTITSAGSHVFTSTGRGGSAGAIDLQ
                     ANQGAITISGTLSNNAGYNRVNSARHGSGAAVNLSAANITTQAITTSATSTRINASEQ
                     VSLGAFNSNTPCSGLCSGDLIIDAAPMSAGINLAQSAAWQVKGATQLNLGTAGQANLP
                     QVNNQFDARNSGKTLGITADSAVITSRADVHLTDTRVNSLALHTLGNLNQAAGSRIYV
                     SGDASLSAENISLSEANNDWSVLHNLTGVNSVALSDLNGLVIDGLDTPLLTLNTPAEA
                     ALSGALRLHNTTLITQHGPGGLRLNGGISRTGTGELQLRATAPGAPLITAGAEDTLWH
                     LTAENQGTLAIKGEYFGQFAGFSALIAVGSGQNVLSGADQDNLWRIFGNATGSIGAVG
                     FSGMSHLQGGAAADRFELQHNSDTSVLTYDGGEGEDTLVNNWLPLNSWHMTGENQGYI
                     SGAAETVRVKAFANIEHLQGLGDRLYGPEGDNQWAISATNAGSLNTQLSFSGMAELIG
                     GSGSDRFAFSDRKFITEGISGGAGVDEVVAAAADWSVSAQGSGFFAYPNSTARQSFRD
                     LEVLTSTAPGTLTGPVAGGTWRIRGNNSVTLGNLTFNGMASLQGNSGADRFELQRDSD
                     TTVLTINGGEGRDELVNNWLPFNTWYITGANEGYVSGAQNRLRVKGFADIERIQGYDD
                     RLRGPDADNQWDITATNGGLLNGALTFAGFTELRGGAGSDHFQITAGKYITEGIFGGA
                     GADDLSVRESTSESSGNAWTITAAGRGNLVFDYLNRFNQQAFYEIETLRGAEAADIFR
                     VRSTGIAMTLDGGANYDQVEFTGQQDITLGQPTSEGINLIDIESLSGDPLTENTLRSA
                     SSGGQWELQPGHLTSAGLAVDFSNFSHLIGGGDGDHNPLFVSPSLNALILREGEYNGQ
                     IVFGASHIKFDNISLLQPIQVD"
     gene            complement(7623..8735)
                     /locus_tag="PKZ52_03980"
     CDS             complement(7623..8735)
                     /locus_tag="PKZ52_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNG58961.1"
                     /translation="MRPFLVRLHRYIGLLIAGFLLLCGFSGALLAWQEELDRWLNPHW
                     YQTSPNHQPRPALDLVEQFEREHPGIEVTSLPLLIKPGQPLKVWVQARPPAAGLGFNQ
                     VFIHNQSAQLLGARTWGKVRLDRAHLIPLLYKFHYSLLIPGQWGIWLLGMVAVLWFLD
                     CFSALAISFPRLDLWRKSLRFRLSSGTPKRVFDLHRSSGVWLWLLLTPFACTAISMNL
                     QQELMRPVVGIFSPLAPSPYDRPLPGGELPPLSRALVVQKAQAAIPAGHNAAPASLSC
                     ISALNLCSVEFRYTPERLINSRVFLDARDGAITQVQGENDSPGDKFLSLQFALHSGQI
                     AGTPGRLIVTLLGLAIAALCSTGILIAWRKYRARRG"
     gene            8959..9492
                     /locus_tag="PKZ52_03985"
     CDS             8959..9492
                     /locus_tag="PKZ52_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNG58962.1"
                     /translation="MAENATIYKLTLTLADLDRQVYGDYNLTLALHPSETVERMMVRV
                     LAFCCRAEPNLAFTKGLSSTEEPALWRKHDNGTLLEWIDVGQPTPERVKKASNQAQRV
                     SIFSFGRGVEVWWKANAAAIKALPKVTTYYLAAEDVRALAALADKTMKLTVTLTEGLA
                     YVANAQESVTLTVLEQQ"
     gene            complement(9620..10507)
                     /locus_tag="PKZ52_03990"
     CDS             complement(9620..10507)
                     /locus_tag="PKZ52_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNG58963.1"
                     /translation="MSEATFYGALEVGGTQCNALIVDEHRKVCAQFHIPTTDPTPTLE
                     QIADFFRKQQQTFPIKALGIGCFGSLDVNPNSPGYGTILNAPKTHWSQVALRQFLSEQ
                     LQVPVLITTHENAAAMGEYFWGHARGLKNFIYVSVGTGISGGVFVDGKPMQGIGHPEI
                     GHMLVPRHRKDLQFFGCCKFHGHCLEGLASGTAMAKRWGAQAQQLRADSPAWALEGFY
                     LGVMCHNLCMHFCPQKIIIGGGVMAHGGLITLVQQEFMSELNGYSPSHLIHHVEDFVV
                     LPKLGNQVGLVGALGLVLH"
BASE COUNT         2394 a         2908 c         3031 g         2443 t
ORIGIN      
        1 gaggcgaacc ttgcggattt gatcgccaag gtgaaagagg aaaacgcaga cctaggtgtg
       61 gcctttgacg gcgatggcga ccgcctggtg gtggtgacga gcaccgggca gattatctgg
      121 ccggaccgtt tattgatgtt gttcgccaag gatattctgg cccgcaaccc cggcgccgac
      181 gtggtcttcg acgtgaaaag ctcccggcta ctcaccagcg tggtggccag tcaagggggc
      241 cgccccatta tgtggaaaac cggccattcc cccatgaaag ccaaggtgca ggagaccggc
      301 gcgctgttgg gcggtgagta ctccgggcat atctttatta aggatcgctg gtacggcttt
      361 gacgacggca tgtacgccgc cgcccgcctc atcgaaatca tgagcctacg ggagcagagc
      421 ctggacgata ttttcgcgga attcttaccc ctgccgtcca cctctgaata ccgcgtaaag
      481 gttgccgaag aagaaaaaat ctctatagtg cagcgcctga tcgaggaggg ggattttggc
      541 gacgccaagc tcattaccct cgatggcgtg cgtgtggaat acccctacgg ttggggcata
      601 gtgcgcccct ccaataccga aagctgcctc accctgcggt ttgaggcgga gaccgaggcg
      661 gacatccaca aactcaaggc gctgttcacc cgcgagctgc gcaaaatcga caatcgcatc
      721 tccttcgcct ggtaagccca agtggtggcc gttgggctgg aggaggcaag gtttaagagg
      781 tttatatgac agcacccaaa gacgccagcc aaattgcccg ggtattaacg gaagccctgc
      841 cctatattca gcgcttcacc ggcaaaacca tcgtagtgaa atacggcggc aatgccatgg
      901 acggagcgga gatggaaaac tccttcgccc gcgacatagt gctgatgaaa caggtgggca
      961 tgaaccccat cgtagtgcac ggcggcggcc cgcaaattgg cgagctgctc accaagctga
     1021 atattcagtc ccactttgtg gacggcatgc gcgtcaccga tagcgccacc atggacgtgg
     1081 tggaaatggt attgggcggc agcgtcaata aacacatagt caacctcatc aaccgcaatg
     1141 gcggccaggc cataggcgtt accggcaaag acggcaacct aatcaacgcc cgcaaaatgg
     1201 aagtcacccg ctactgcccg gagctaaaag cccccgaaat tatcgatata ggccaggtgg
     1261 gggaggtgcg ctcaatcaac cgcaaagtga tcgacctgtt ggtgcagagc gatttcatcc
     1321 cggtgattgc ccctatcggc gtgggcagcg acggcacctc ctacaacatc aatgcagact
     1381 tagtggcggg caaggttgcc gaagtattgc aggcggaaaa acttatgctg ctcaccaacg
     1441 taagcggcct gctggataaa cagggccagg tgctcaccgg ccttaccacc gcgcgggtgg
     1501 atgagttaat tagcgacggc accatttacg gcggcatgtt gccgaaaatc cagtgcgccc
     1561 tagatgcggt gaaaaacggc gtacacagtg cccatatagt ggatggccgt gtagcccatg
     1621 cggtgctgct ggagatcttt acggatgcag gggtgggcac tttaattact aaccgccact
     1681 aaacctcacc gcttagcggg tgaagagtta tccgctaagc gagtcaaagg tattagcgct
     1741 tcctctgcgc cgatcaacca acgcttttca ccttctcccg ctaaggcctg tttggcggtc
     1801 gggcaggcct tgcatcgctt ccagagtgtt taatccacct gaataggctg cagtagggaa
     1861 atgttatcga acttgatgtg gctagcgcca aagacgatct gcccgttgta ctcaccttca
     1921 cggaggatca gagcattgag cgatggggac acaaagagcg gattgtgatc gccatcgccg
     1981 ccgccaatca gatgggagaa attactaaaa tcaaccgcca gccctgctga ggttaaatgc
     2041 cccggctgca gctcccactg cccgccgcta ctggcagagc gcagggtatt ctccgtcaga
     2101 ggatcgccgc tgaggctttc aatatcgatc agattaatac cctctgaggt tggctgcccc
     2161 agggtgatgt cctgctgccc ggtgaattcc acctggtcat aattggcgcc gccatcgagg
     2221 gtcatggcga tacctgtgct gcgcacccga aaaatatcgg cggcctcagc gccccgcagg
     2281 gtctcgatct cgtaaaacgc ctgttgatta aagcggttga gataatcaaa taccaggttg
     2341 ccccggcctg cagcggtaat cgtccaggca ttgccggagg attcgctggt gctttcgcgc
     2401 acgcttaagt cgtccgcacc cgcgccgcca aaaataccct cggtgatgta tttgccggca
     2461 gttatttgga agtggtcgct gcccgcgccg ccgcgcagct cagtgaaacc ggcaaaggtg
     2521 agggcgccgt ttaagagacc gccgttggtg gcggtgatgt cccattggtt gtccgcgtcc
     2581 ggcccgcgca ggcgatcgtc ataaccctga atgcgctcga tgtcggcaaa gccttttacc
     2641 cgcaggcggt tttgcgcgcc gctcacataa ccctcattgg cgccggtgat gtaccaggta
     2701 ttaaatggca gccaattgtt caccagctca tcgcggccct caccgccatt gatggtcagc
     2761 actgtggtgt cgctgtcgcg ctgcaattcg aagcgatccg cgccgctatt gccctgtaag
     2821 cttgccatgc cgttaaaggt gaggttgccg agggttacgc tgttattgcc gcgaatgcgc
     2881 caagtgcccc ctgcaaccgg gccggtgagg gtgccagggg cagtgctggt taaaacttcc
     2941 aggtcgcgaa acgactggcg agcggtagaa ttcgggtagg cgaaaaagcc gctgccctgg
     3001 gccgatacag accaatcagc ggcggccgcc accacctcat ccacgccagc tccgcctgag
     3061 atcccctcgg taataaattt cctgtcgcta aaggcgaagc gatcgctgcc gctgccgccg
     3121 attaattccg ccatgccgct aaagctgagc tgggtgttga ggctgcctgc gttggtggcg
     3181 ctgatggccc actggttatc gccctctggg ccgtacaggc ggtcgcccaa gccctgcagg
     3241 tgctcgatat tggcaaaggc ttttacccgc acagtctcgg cggccccgga gatatagccc
     3301 tggttttccc ccgtcatatg ccaagagttt aacggcagcc agttgttgac cagtgtgtcc
     3361 tctccttcgc cgccgtcata ggtcagcacg gaggtatcgc tgttgtgttg cagttcgaag
     3421 cgatctgccg ccgcgccgcc ctgcagatgg ctcatgccgc taaaacctac cgccccgatg
     3481 ctgcctgtgg cgttgccgaa gatgcgccac aggttatcct ggtcggcccc gctgagcaca
     3541 ttctgcccgc tgcccacggc tattagcgca ctaaaaccgg caaattgccc gaagtattca
     3601 cccttaatgg caagcgtgcc ttggttttcg gcggtaaggt gccacagggt atcctctgcc
     3661 cccgcagtga ttaagggtgc ccccggcgca gtggcgcgca gctgtaattc gcctgtgccc
     3721 gtgcggctga tgccgccatt taagcgcagg ccgcccgggc catgttgggt aattagggtg
     3781 gtgttatgca gcctaagcgc gccgcttaat gcggcttcag cgggggtgtt aagcgtaagc
     3841 agcggtgtat cgagcccgtc gatcacaagg ccgttgagat cgcttaacgc gacggaattg
     3901 acccctgtga ggttgtgcag cacagaccaa tcattattgg cctccgataa actaatgttt
     3961 tcagcactta agctggcatc gccactgaca taaatacgcg aacccgccgc ctggtttaaa
     4021 ttccccaggg tgtgcagggc gagagaattt acgcgggtgt cggttaagtg cacatcggcg
     4081 cggctggtga tgacggcgct gtcggctgtg atgcccaggg ttttaccgct attacgtgca
     4141 tcaaattgat tattgacctg cggcagattg gcttgcccgg ccgtgcctaa atttaattgg
     4201 gttgcgccct tcacctgcca agccgccgat tgcgcaagat taattccagc gctcatgggc
     4261 gcggcatcaa taattaaatc gccgctgcac aggccgctgc aaggggtgtt ggaattaaac
     4321 gcgccgagac tgacctgctc gctggcatta atacgggtgc tagtggcgct ggtagtaatg
     4381 gcctgggttg taatgttggc ggcggataaa ttaacggcag cgcccgaacc gtggcgagcg
     4441 ctattaacgc ggttgtagcc tgcgttattg ctcagcgtac cgctgatagt aatggcgcct
     4501 tgattcgctt gtaggtcgat ggcgcctgca ctgccgccgc gccctgtgct ggtaaatacg
     4561 tggctgcctg cgctggtgat tgtattgagc acaatagaac cggtggccgc atttaattgg
     4621 attttaccgc tgttattatt ggtttcgccg ctgcgcgcgg tactgagtgt cgctttactg
     4681 gtaatagaac cgttttgggt gcgggccgag agtgtgccgc cgttggtggc aagattggcg
     4741 cggttaaaat taatgccgct ttgggcatta aattccatat tcagccggtt atttgtcgag
     4801 gcgcgcagat aagtaacgct atcggtaaat tcgatatttt tatcggcggt taaaattaaa
     4861 ctggcattaa atccactttg cggcgcactg ctgcgcaggg tggaaaaatc aaaattagcg
     4921 gccactttaa tagtaccacc ggcattgctg ccgccgctgg ttttaatttc caccgtaccg
     4981 ccgctgccgt tatccaacac gttgcgaata ctattccaac caatttgcgc cgcgtcattg
     5041 cgggaggtaa atagcgattg ggtttgggtg gcgttagttg acgtactaat tcgatccggc
     5101 cgcagaacag cgctatttac aatttcaata ttatacgggt cgattaacca gtggcccgca
     5161 acgcctgcag gggcgctaat atctggcgag ttccacacct caaggccgtg taagccagag
     5221 gtttctataa aaccgccatc gccgtgatcg ccaccccggg cggataaatg gccgtaaata
     5281 gcggcgctgt cctcggcaaa tacaattaat ttgccgccgc tgcctgtgtg cagcgcatcg
     5341 gtgcgcacct gcgcctgctc gcttatatgg gtaaattcgg cattgttaat gagagggtta
     5401 gcacctgttt tatcgccgcc aattaatatc tgcccgccgc cctcagcgcc ggaggcatct
     5461 acctgcgctt cgtctaacaa cccaacttgc tggccaagca ttttaatatc accgccctgg
     5521 ccttgcttat tggccctagc gcttaagctg ctagcacctg tgtttaaagt ggttttggtg
     5581 gcatttaatt caatggaacc agcatcgccc agggtattat cggcgttaat agtgccgcta
     5641 tgggtaatgt tttctcctaa cacaacggct gcgcctgcgc ccacattaat cgcgccggta
     5701 ttctccacat ccgcacctgc gccgagacta aaactgccgt cggcattcac caccgcgctt
     5761 ttagcctgat ttaattcacc cacattcacc gcttggctaa aaatattttt actcacacta
     5821 gcagttaata atactttgcc gccctgagcc gtaatgtctc ctttattaat aacggcggca
     5881 tttacaccca aatcattttg cagcaccgcc tcgctcaccc gcacaccaat taatccgtcg
     5941 cgttcgaatg taagtaccgc ttctttgcca gcggcaagat gcacactacc taaattagcg
     6001 ctgatcaagc cgctattttc cacccgttgg ccgagcaaat taatgctgcc gccggtggcg
     6061 gcgtttaaca gcccgctatt aataacctgc ccactggcgc cttccagggc atttagagtg
     6121 aattggctgt tgagaaattc ctcgttatta atatccaaac ccgaagctac caaagcgccc
     6181 acattgacgc tggcggtttc agtaaaaata atgccgttgg gattaattaa caccacctgg
     6241 ccattggcat caattcggcc ctgaatcagc gagcctttgt tacttaatat gcggttaaag
     6301 gcgatggagg attccgctgg ctgaataaac tgcacgcgct catcgctttt aatatcaaaa
     6361 ttctgccaat taatactcag gcgctcgctg gcctgttgaa taagcgtatt tttgccctgc
     6421 agctcaatgc tgccttgccc gcctatgatt tggccttgct ccggggctgc ccagagcgtt
     6481 ggaataatgc ttatggttgc gagggcgcag gtgagttttt ttaatggata gggtacgtag
     6541 ggcaatcggt tcatagcggg gactcatagc ggtgtgaagg taaaaattaa ttattcgtcg
     6601 tgcctaaagg cgtaggtgaa tgtttaatta attgttcggc atctgctctg aacgcttgcc
     6661 tacctggttg gcgaagatgg cggtgccatg agtgcaggtc gcttgcctgt gccggggcgt
     6721 aggtggcacg cagcaagaaa aataacagct caggcattgc gcccagttgt tatttatcaa
     6781 ttggtgttta attaaaaatg agtggcccat gttatttttc cttttgcctg ttgtgtcacg
     6841 ggaacagtag atatttaccg attcgttttt caattctgcg ggttgccacc cccgccaaca
     6901 cccgcaatag cttttggctc agcgggcatt ttattaaata gcgtttggct atccccgtgt
     6961 tcaatttata tagcgaatta ggtgttctgc actgcgaaaa tacagtgagc gaaaatcact
     7021 gtagacaagc tcaaaagcga tagcaacgta aaaattaccc agctatcaat ggcgtatatg
     7081 aaatcgacgg ttagtgctta ctaagcgcta tgggctcaag gccccgcagc tgagaagaag
     7141 gcttattccc gagccgatca aaatgactga attgagccta aaaatattta tttcggcgtt
     7201 ttttcacaaa gttaatttgt gagcgaattg ccattttctc ctcctgttgt gcacagtagg
     7261 gcgggggatg cagtgcatgg attcaggtgc tgttcgttcg agactggccg tctggggcaa
     7321 attattaacc cggcgggtta atcttaagag atgaacttaa gcgatggcac aattttttag
     7381 aatgcagacg caagtggaac agggcaattt tttatcctaa aaatcgcagt agagttcaac
     7441 tgcggttttt aggtttattg caggcctagg ctatggcgga tgcttgagcc ttaaaaacac
     7501 gatttatttc acctgtgccc attggttggg gatagtagat gaaggaccac tgcagaaaaa
     7561 agtatggagc tttgtcagcg ctgcacctat aggctatggg tgcattttca atctggcagg
     7621 cctcagccgc ggcgcgctcg gtacttgcgc catgcgatca aaatacctgt actgcagagt
     7681 gctgcaatcg caagtccaag tagtgtaacg atcaaccggc caggggtgcc cgcaatttgg
     7741 ccgctgtgca gcgcaaactg caggctcaaa aatttgtcgc cggggctgtc gttttcccct
     7801 tgcacctggg taattgcgcc atcgcgggca tccaaaaaga cgcggctatt gataagccgc
     7861 tcgggtgtat agcgaaattc cacactgcag agattaagcg ctgatataca gcttagtgat
     7921 gcgggcgcgg cgttatggcc ggccgggatt gccgcctggg ccttttgcac cacgagcgcc
     7981 cggctgagcg gcggcagctc gccacccggc agggggcgat cataggggct gggggctaag
     8041 gggctgaaga tgccaaccac aggccgcatc aattcctgct gcagattcat gctaatagcg
     8101 gtgcaggcaa atggcgtcag cagcaaccaa agccaaacgc cgctggagcg gtgcaggtca
     8161 aatactcgtt tgggggtgcc gctcgatagg cgaaagcgca gggacttgcg ccatagatcc
     8221 agcctgggaa aggaaattgc caaggcgcta aaacagtcta gaaaccacag caccgccacc
     8281 atgcccagca gccaaatgcc ccattggccc gggatcaaca gggaataatg aaatttgtat
     8341 agcaggggga tcaggtgtgc ccggtcaagt cttaccttac cccaggttcg cgcccctaat
     8401 aattgagcgc tctggttgtg aataaacacc tggttaaagc ccaaaccggc tgcgggtggg
     8461 cgcgcctgca cccagacttt tagcggctgg ccgggtttta tcagcaaggg taggctggtg
     8521 acttcaatgc ctgggtgctc gcgctcaaat tgctcgacta gatccagagc gggccggggc
     8581 tggtggttgg gggatgtttg ataccagtgc gggtttagcc agcgatcaag ctcctcctgc
     8641 caggctaaaa gcgcgccgct aaagccgcat agcagcaaaa atcccgcaat caaaaggccg
     8701 atatagcggt gaaggcgaac aagaaatggg cgcatagggg cagagttaat aaaatagaag
     8761 tacgccagcc ttggcatagg cgcggccatt ttggtgggtt tgcactttta tgctcggcga
     8821 gccagcaggg cgtcattcta gccgaagccg cctgcgggca agggtaagat ctgccctcaa
     8881 ttccccccgt tttaggccgc gccaaatccc agcaactgag gtactctgcc gccgcacaca
     8941 attttctgtg agaaccccgt ggccgagaac gcaaccatct acaaactcac cctcacgctt
     9001 gcggatttag accgccaggt gtacggcgat tacaacctta ccctggcgtt gcatccgtcg
     9061 gagactgtgg agcgcatgat ggtgcgggtg ctggcgtttt gctgtcgggc cgagccgaac
     9121 ctggccttca ccaaggggtt gtcttccacc gaggagcccg ccctgtggcg caaacacgat
     9181 aacggcacgc tgctggagtg gattgatgtg ggccagccca ccccggagcg ggtgaaaaag
     9241 gcctccaatc aggcccagcg ggtgagtatt tttagttttg ggcgcggggt agaggtgtgg
     9301 tggaaagcca acgccgccgc catcaaggcg ctgcctaagg tcaccactta ttacttggcg
     9361 gcggaggacg tcagagccct tgcggccctg gcggataaaa ccatgaagct caccgtcacg
     9421 ctcaccgagg gcctagctta tgtggccaat gcccaggaga gtgtgaccct gacagtgctg
     9481 gaacagcaat gagctggcgc tcgggaaatc agtaggcgct caaactcccc gctcaagctc
     9541 aagctcaagc tcaagctcaa gctcaacgct tagccttaag cctaaaaacc gcagtagagt
     9601 tcaactgcgg ttttaaggct tagtgcagca caagccccag cgcccccacc aatcccacct
     9661 ggttgccaag ctttggcagc accacaaaat cctccacatg gtgaattaaa tggctggggc
     9721 tgtagccgtt gagctcactc ataaactcct gttgcactaa ggtgatcagc cccccatggg
     9781 ccataacacc gccgccgata atgatctttt gcgggcaaaa atgcatacac aggttgtggc
     9841 acataacgcc tagataaaac ccttccaagg cccaggccgg gctgtcggcg cgcagttgtt
     9901 gcgcctgggc gccccagcgt ttggccatgg cggtgccgga ggcgagcccc tccaagcagt
     9961 ggccgtggaa tttacagcag ccgaagaact gcagatcctt gcggtggcgg ggcaccagca
    10021 tatggccgat ctccgggtgg cctatgccct gcatgggttt gccatccaca aacacgccgc
    10081 cgctgatgcc ggtgcccacc gatacataaa taaaattctt caaaccccgc gcatggcccc
    10141 aaaaatattc ccccatggcg gcggcgtttt cgtgggtggt gatcagcacc ggtacttgca
    10201 attgctcgct taaaaactgc cgcagagcca cctggctcca gtgggttttc ggtgcattga
    10261 ggatggtgcc gtagccgggg gagttggggt ttacatctag ggagccgaag cagccgatgc
    10321 cgagggcttt gatggggaag gtttgctgct gtttgcggaa gaagtcggcg atttgttcca
    10381 gggtgggagt ggggtctgtc gtggggatat gaaattgggc gcagactttg cggtgttcat
    10441 cgacgatcaa ggcgttacat tgggtgcccc ccacttccag ggcaccgtaa aaggtggctt
    10501 ctgacatagc ggtctcgttg caggttgggt accgggcacc cggcgcacca gggtggcttg
    10561 ggcacggttt ctaaccttga gtataggcga ggctggcaaa agtgattatt tccttaacaa
    10621 gcaggccatg gggaaaatgc caaacttttt tcttattttt tcggcatagg tcgccttaaa
    10681 atttagaaca aaacggtcta caaatcggaa tcctaaggct gttcggtaag ggcggctcaa
    10741 gggcgcactg ctgcgggtga gcaaccactt atcccc
//
LOCUS       DAOQGS010000068        10770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2849_length_10770_cov_4.964069, whole genome shotgun sequence.
ACCESSION   DAOQGS010000068 DAOQGS010000000
VERSION     DAOQGS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_10770_cov_4.964069"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PKZ52_03995"
     CDS             <1..627
                     /locus_tag="PKZ52_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNG58964.1"
                     /translation="LCQLIFHPGLSTAEKITNLSGRGVGMDVVKRNIESLRGTIEIET
                     ELGVGTTMSICLPLTLAIIDGFHVAAGKIDFIVPQNTILECIDLNTLSHTQEQNCVNL
                     RGEQVPFIRLQELFRLERQEHVQEKLVVVQFGEKRAGIAVDSLFGETQTVVKPMGPIF
                     QALKGIGGSSLLGTGTVALILDVQQLIQFAISKEYERNAHSIAGIQES"
     gene            629..1141
                     /locus_tag="PKZ52_04000"
     CDS             629..1141
                     /locus_tag="PKZ52_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNG58965.1"
                     /translation="MSVQRKAKKAIVHAVATKQFLTFRIGNEQYGLELAQTREIIEYG
                     GITSVPLMPNFLRGVINLRGEVVPVIDLAVRLGRKPVEVQKRTCIIVIELNNNEQNHV
                     LGLLADAVSEVIEIDDDNIEDAPSFGANIRADFIQGIAKRDDEFVVLLDANNTLSIRE
                     LAHLVEADLQ"
     gene            1157..2722
                     /locus_tag="PKZ52_04005"
     CDS             1157..2722
                     /locus_tag="PKZ52_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG58966.1"
                     /translation="MLKNMTLKAKILMLSALIVLGLSVLGVSAYVQMRQFNAVVNETN
                     VHVLKRSSILAEVQGAAVEFKTQVQEWKNILIRGNAPEQYARYLEGFGQSAQGVQEHL
                     QKTLAMQKEEHLDTAEVERLLKEHSQLGVNYRDALKKFDQNDANTGKLVDKLVSGMDR
                     EASKQMEALAAATSAGFAQYLRESNQKTEQIYADTLRLLVLLCLCASLVIVGVMAVIF
                     RDLFVILGGEPAYTAQVVSQVAEGYLNTKVQLKPGDQGSLLASVANMQKRLADIIGDV
                     SRSADALSSAAEQVNATAQSLAKGASVQAASVEETSASMEEMSASIAQNTDNAKATNS
                     MAQRASQDAATGGEAVMSTIAAMQKIAERISVVDDIAYQTNLLALNAAIEAGRAGEHG
                     RGFAVVASEVRKLAERSQIAAQEISTLAIDTVKRAEHAGKMLNEMVPAIRKTADLVQE
                     IAAASNEQNSGVTQINSAIAQVNQTLQQNAAASEELSATSEEMSTQAISLQEAMTYFK
                     LFNRDIRAHSMRM"
     gene            2926..4557
                     /locus_tag="PKZ52_04010"
     CDS             2926..4557
                     /locus_tag="PKZ52_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG58967.1"
                     /translation="MTVAKKMMFLIGAGLLGIFILAGAAQIQIEKVFEETNYTNENVL
                     PSMILLAKIQNDIYRTRIRLNRHILNTDLSQSKQIEQTMNEAIANVRSGFKDYEAMLA
                     DDKDKELLKAETDSFERYLKHIPAIIAVSEKNQTDQARDLMEASVQDAQGASKAVDDH
                     MQYNIQIAENAKNRALASKKTAALVSIIVASLTLISIAVIGFFITRSLLRQLGGEPAY
                     AVDIVGRVADGDLNVQVNLKPGDDSSMLAAIKNMTERLIEVISDVRGSADALASASEE
                     VNATAQSLAKGASIQAASVEETSASMEQMSASILQNNENASVTDGMAQKSARDAVSGG
                     EAVAATVAAMQKIAERIGVIDDIAYQTNLLALNAAIEAGRAGEHGRGFAVVASEVRKL
                     AERSQVASQEIGQLATDSVKRAELAGVLLQDIVPSIRKTADLVQEIAAASTEQTTGVN
                     QINAAMGQVNQTMQQNAAASEELSSTAEEMTAQAMRLQEAMAYFKLANDRHHELAHGS
                     RALKNKMTGAKPLAVKTKSAYALEDDADRHFGRFD"
     gene            4660..5478
                     /locus_tag="PKZ52_04015"
     CDS             4660..5478
                     /locus_tag="PKZ52_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNG58968.1"
                     /translation="MLLEKAHIMPSDQEFMAFQKLILERIGIFLPMSKKALLMNRLWK
                     RLSAHKLNSYHEYYCFIVSKKGSQELNLALELMTTNETYFFREQKHFDYLANDILPQF
                     KPRDTLRIWSAAASTGEEPYSIAMLLADRCAANWELLCSDVNSQVIEHAKIGIYPNLR
                     VKNIPEHYLRRFCRKGSGPQEGFVRVVADLRERVEFFTLNLNVPFARELGLFDVVFLR
                     NILIYFENDVKEQVLNRVASTLKPNGILFIGHSESLHGVTNKFSPIKPAIYKRA"
     gene            5497..6009
                     /locus_tag="PKZ52_04020"
     CDS             5497..6009
                     /locus_tag="PKZ52_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNG58969.1"
                     /translation="MAKTPNYMRLNIHPGEWYVGARYHSIYTVLGSCVSVTAWHPELK
                     LGGMCHFILPTLPRGLKDIKVNAGRYANTALEMMKLALSAQAPLTEFQLGLFGGSDTI
                     THFDVGKNNVIYAQQWLAAEQLNANRIDIGGRTSRSVTLHISSGVLIVKHHNMPIINP
                     LPAGINTKRL"
     gene            6012..7052
                     /locus_tag="PKZ52_04025"
     CDS             6012..7052
                     /locus_tag="PKZ52_04025"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNG58970.1"
                     /translation="MGIEVLVVDDSAVMRQVLREILNEAPDINVYAAAPDPIFALQKM
                     QGQWPDVIVLDIEMPRMDGLTFLRKIMAERPTPIIICSSLAKKGTELSLQALAAGAID
                     VITKPQLGVKDFLLTAKQLLWTTVRGAATARMARHRVEREPVAVAQPPVVADLVALTS
                     TTDRITVIGTSTGGTQALEEILVSVPRTAPGIAVVQHMPELFTAAFAQRLNSICTVEV
                     KEASSGDRLIPGRVLIAPGGHHLEIQRSGAQYIAKVFRGPAVNRHCPSVDVLFRSAAV
                     AAGRNATGIIMTGMGDDGARGLLDMRQAGARTIAQDEASCVVFGMPKEAINLGAAKET
                     LSLSKIAKVLES"
     gene            7055..8287
                     /locus_tag="PKZ52_04030"
     CDS             7055..8287
                     /locus_tag="PKZ52_04030"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNG58971.1"
                     /translation="MKTSLAELHILIADDSNTQRQFAQMHCNNLGIEHIYLAADGMES
                     LHILQNNAIDAVLIDLEMPIMDGVELIHAIAQHKLSPYIIIVSAKDPILIASVGTMAE
                     AEGLRVLGTFKKPLQTEMLQCSFLHLLTALQALNYDNLVPAPNPAQMAVTGLELSQAI
                     NNNEIFLAYQPKLSVQGLLLCGVEALARWQHPEKGLIAPVVFIPMAERLGLISALTQK
                     LFVLALEEKRKWQRMGFHFHLAFNLSPYSVSDEGLADWIVATAKQYDVPPQEIIFEIT
                     ENALLNELACAIRTLARLRLKGFSIAIDDYGTGFANAQQLSRVPATELKLDRSLVHNV
                     SQRPQQLTILSSSIELGKKLNLTLVAEGVENRDDYNVLANLGVDQVQGYYFSRPLYPD
                     DLLNFVKTRLSEIRRATQ"
     gene            complement(8376..8834)
                     /locus_tag="PKZ52_04035"
     CDS             complement(8376..8834)
                     /locus_tag="PKZ52_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNG58972.1"
                     /translation="MDKFDKAILATLQRQGRISNQELADAIGLSPSPCLRRLRALEEA
                     GFIDSYGARLNGRKLGLTLTAFIQISLDRHTPERFAAFEKKLTKLPEVLECHLITGQQ
                     ADYLLKVIVKDMDAYQHFLLNKLTTIEGITGVHSSFVLKSPVQDNHLPLD"
     gene            8967..10646
                     /gene="leuA"
                     /locus_tag="PKZ52_04040"
     CDS             8967..10646
                     /gene="leuA"
                     /locus_tag="PKZ52_04040"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNG58973.1"
                     /translation="MLKNPAEKYRPFKGVALPDRTWPNRQITRAPIWMSTDLRDGNQA
                     LMDPMNVTTKLRFFKHLVAMGFKEIEIGFPSASQIDWDFARLLIEGKHIPQDVTIEVL
                     TQARLDLIEKTVASVAGAKQVILHMYNPVAPAFRRIVYNTDQAGVKAIAEQGTRWVKE
                     ITARYPQTQWTYQYSPEVFSGTEIEFAKEVCDAVSAIWQPTPERKMIINLPATVEMGT
                     PNTYADQIEWMHRYLDNRASIILSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGE
                     RTGNVDLVTLAMNLYSQGIHPGLDFSNMDQVRQLVEECTQLPVHPRHPYAGELVFTAF
                     SGSHQDAIKKGFAQQQPGSVWEVPYLPIDPQDVGKSYDAVVRVNSQSGKGGVSFLLQQ
                     EAGFNLPRRMQIEFSAIVQRVSDNTGREVKSSDIVALFEQEYFAQTKPFVYHYNEIKA
                     VNASDDPVRVDIHGAFRQEAVVYSGTGNGPIDAAANALSQFTGQSIQVVDYHEHALTT
                     GSQSQAVCYLEVKVGGDKAFFGVGVDKNIIAAAIKALFNGVNRKQKYSQAC"
BASE COUNT         2864 a         2387 c         2775 g         2744 t
ORIGIN      
        1 ttgtgccagt taatttttca ccccggttta tctaccgctg aaaaaattac caatttatcg
       61 ggccgtggtg tgggcatgga tgtggttaag cgcaatatag aatccctgcg cggcactata
      121 gaaattgaaa ccgagttggg cgtaggcact actatgagta tttgtttgcc gctcaccctg
      181 gcgattattg acggctttca tgttgcagcg ggcaagattg attttattgt gccgcaaaat
      241 accatccttg aatgcatcga cttaaacacc ctcagccata cccaggagca aaactgcgtg
      301 aatttgcgcg gcgagcaggt gccttttatc cgtttgcagg aattatttcg gcttgaacga
      361 caagagcatg tgcaggaaaa attagtggtt gtacagtttg gcgagaaacg cgcaggcata
      421 gccgtggata gtttgtttgg cgagacccaa accgtagtaa aacccatggg gcctattttt
      481 caggcgctta aaggtatcgg tggctccagc ttgctgggga caggaacagt ggcactaatt
      541 ttagacgtac aacaactaat acaatttgcc attagtaaag agtatgagcg caacgcccac
      601 tcaattgccg gcattcagga gtcataatat gagcgtacaa cgaaaagcaa aaaaagccat
      661 tgtgcatgcc gttgccacca aacaatttct cacctttcgt atcggcaacg agcagtatgg
      721 ccttgaactg gcgcaaaccc gcgagattat tgaatacggc ggtattacta gcgtgccatt
      781 aatgcccaat tttttacgcg gtgtaattaa cttgcgtggc gaggttgtgc cggtgattga
      841 tttggctgtg cgcttagggc gcaaacctgt ggaagtgcaa aaacgcacct gtattattgt
      901 gattgagctc aataacaacg aacaaaacca tgtgttgggt ttgttagcgg atgccgtaag
      961 tgaagtgatt gaaatcgacg atgacaatat tgaagatgcg ccgtcttttg gggccaatat
     1021 tcgcgctgat tttattcagg gaatcgccaa acgcgatgat gaatttgtgg tgctgctcga
     1081 tgccaataat accttatcaa ttcgcgagct agcccatttg gtggaggccg acttacaata
     1141 aaaaaagggg tacaccatgt taaaaaatat gacgttaaaa gcaaaaatac ttatgctcag
     1201 cgccttaatt gtgttgggcc tatccgtctt aggtgtttcc gcctatgtgc agatgcggca
     1261 atttaatgcg gtcgttaatg agaccaatgt acacgtttta aagcgctcga gcattttagc
     1321 tgaggtgcag ggcgcggctg ttgaatttaa aactcaagtg caggagtgga aaaatatttt
     1381 aattcgcggt aacgcgccgg aacaatatgc ccgttatttg gaagggtttg gccagtcggc
     1441 gcaaggtgtt caggaacatt tgcagaaaac ccttgccatg caaaaagagg agcatctgga
     1501 tactgcagaa gttgagcgtt tgttaaaaga acacagccag ctgggcgtta attatcgcga
     1561 tgcgctcaag aagttcgatc aaaacgatgc gaatacaggt aaattagtcg ataaattagt
     1621 cagcggcatg gatcgcgaag ccagcaaaca aatggaggcg ctggcagctg cgacctcggc
     1681 aggttttgca cagtatttgc gcgaatccaa ccaaaaaacc gaacagattt acgcggatac
     1741 attgcgctta ttggtgttgc tatgtttatg tgcttcgctg gtgatagtcg gtgtgatggc
     1801 ggttattttt cgggatctgt ttgttatttt aggcggcgag cctgcttaca cggctcaggt
     1861 tgtctcccaa gtagcggaag gttatttaaa taccaaagtg cagcttaaac ctggcgatca
     1921 aggttcgctg ttggccagtg tggccaatat gcaaaaacgc cttgctgata ttatcggcga
     1981 tgtctcgcgc tctgctgatg cattatcttc agctgcagag caggtaaatg cgacggcgca
     2041 gtctttggcc aagggcgctt cggtgcaagc ggccagcgtt gaggaaacct ctgcctctat
     2101 ggaagagatg tccgcatcca ttgcacaaaa tactgacaat gcaaaagcca ccaatagcat
     2161 ggcgcaacgg gcatcccagg atgcggccac cggtggtgaa gccgttatga gcaccattgc
     2221 cgccatgcaa aaaatcgccg agcgcattag cgttgtcgat gatatagcct atcaaactaa
     2281 tttattggca ttaaatgccg ccatagaagc aggccgggcc ggtgaacatg ggcgcggttt
     2341 tgcggtggta gcatcggaag tgcgcaaact tgccgaacgc agccaaattg ccgcacaaga
     2401 aattagcacc ctagccatag atacggtgaa acgcgcagag catgcgggga aaatgttaaa
     2461 tgaaatggtg cctgcgattc gcaaaactgc ggatttagta caggaaattg ccgccgcctc
     2521 caatgaacaa aattcaggcg taactcagat taacagtgcc atagcgcagg ttaaccaaac
     2581 actacaacaa aatgcggctg cctctgagga gcttagtgcc acctccgaag aaatgagcac
     2641 ccaggcaatt agcttgcaag aagctatgac ttatttcaaa ttgtttaacc gcgatatccg
     2701 cgctcatagt atgaggatgt aattgtgctc gcgcgggtat atcgaagcag aatagatact
     2761 ttaactaaaa gaacttctga ttaattagca ttttgggagc cgtcgtgggc agaaagcccg
     2821 cgacgaagct acggggaagc ccctgtgtgt ttctaaaaaa ctctaattta tcagagattc
     2881 ctaaatagta tttagttatt tcacctaagt attagggagt caattatgac cgttgcaaaa
     2941 aaaatgatgt ttttaatagg tgctggattg ttaggtattt ttatacttgc cggggcggcg
     3001 cagatacaaa ttgaaaaggt gtttgaagaa accaactaca ccaatgagaa tgtgttgccc
     3061 agtatgatcc tgctggcgaa aatacagaat gatatttacc gcactcgtat tcgcctgaac
     3121 aggcatatct taaacacgga tctatcccag tcaaaacaaa ttgaacagac gatgaatgaa
     3181 gctattgcca acgtgcgcag tggttttaaa gattatgaag caatgcttgc tgatgataag
     3241 gacaaagaac ttctgaaagc ggagactgat agctttgagc gctatctaaa acatatacct
     3301 gcaattattg ctgtgtctga gaaaaatcaa acagatcaag ctcgggattt aatggaggca
     3361 tctgtgcaag atgctcaagg cgcttcaaag gccgtggacg atcatatgca atataatatt
     3421 cagatcgccg aaaatgctaa aaatcgtgcg ttagccagta aaaaaactgc cgctctagtg
     3481 tccataatag ttgccagcct gacattaatc tcgattgcgg ttatagggtt ttttattacg
     3541 cgcagcttgt tgcgccagtt gggcggtgag cccgcctatg cggtggatat tgttgggcgg
     3601 gttgccgatg gtgatttaaa tgtgcaggta aatttaaagc ccggtgacga ttctagtatg
     3661 ctggccgcca ttaagaatat gaccgagcgt ttaattgaag tgattagtga tgtgcgcggc
     3721 tccgccgatg cgctagcatc cgcatcagaa gaggtaaatg ccactgcaca atccctagct
     3781 aaaggtgcat ccattcaagc cgcgagcgtg gaagaaacct cagcttccat ggaacagatg
     3841 tcggcgtcaa ttttacaaaa taatgaaaat gccagcgtaa ccgacggcat ggcacaaaaa
     3901 tcggcccgcg atgccgtcag cggtggcgag gccgtagcgg caacggttgc ggctatgcaa
     3961 aaaatagccg agcgcatagg cgtaattgat gatattgcct atcaaaccaa tttgctcgca
     4021 ttaaatgccg caattgaagc gggtagagcg ggggagcatg gccgcggttt tgccgtggta
     4081 gcgtcagagg tgcgcaaatt agctgagcgc agtcaagtgg cttcacaaga aattggccag
     4141 ctggctaccg acagcgtgaa gcgggcggaa cttgctgggg ttttattaca ggatattgtg
     4201 ccgtcgattc gtaaaacagc ggatttagtg caggaaattg ccgctgcctc aactgagcaa
     4261 actaccgggg tcaatcaaat taatgccgct atggggcagg tgaatcaaac catgcagcaa
     4321 aatgccgccg cctcggagga gctcagttca acggcggaag aaatgaccgc ccaagctatg
     4381 cgcctgcagg aggctatggc ttattttaaa ttagcgaacg accgccacca tgaattagct
     4441 catggttctc gcgcactcaa aaataaaatg accggagcta aacccctggc ggttaaaaca
     4501 aaatccgcct acgcactaga ggatgacgct gaccgccact ttggccgttt tgattaagcg
     4561 atctgtgtaa gctattggca tgaaattagt tttttcacat acaacctggc ggcttatgca
     4621 tcttgcatta gaccaatatg ctcgtaccat gagggtgcca tgttgctaga aaaagcccat
     4681 ataatgcctt ctgatcagga atttatggcc ttccagaaat taattttgga acgaataggc
     4741 atatttttgc ctatgtcgaa aaaggctttg ttgatgaacc gcttgtggaa aaggctcagc
     4801 gcccacaagc tcaatagcta tcacgaatat tattgtttta tcgtcagtaa aaaaggcagc
     4861 caagagttaa atctggccct tgagttgatg accaccaatg aaacctattt tttccgcgag
     4921 caaaaacatt ttgattattt ggctaacgat attttgccac agtttaaacc ccgcgatacc
     4981 ttgcgtattt ggagcgcggc ggcttcaacc ggtgaagagc cctatagtat cgctatgttg
     5041 cttgccgatc gttgtgcggc taattgggag ctgctttgct ccgatgtcaa cagccaggtg
     5101 attgaacacg ccaaaatagg tatttacccc aatctgcgtg ttaaaaacat acccgagcat
     5161 tatttgcgta ggttttgccg caagggcagt ggccctcagg aggggtttgt gcgcgtagtg
     5221 gcggatttgc gcgagcgggt tgagtttttt acgctcaatt taaatgtgcc gttcgcccgt
     5281 gagttgggct tatttgatgt ggtatttctg cgcaatattc ttatctattt tgaaaatgat
     5341 gtaaaagagc aagtattaaa tcgcgtggcg agcacgttaa agccaaatgg cattttattt
     5401 atcggtcatt cagaaagcct acatggtgta acaaacaaat tttcacccat taagcctgct
     5461 atttataagc gtgcttaaag aaagcttggt gatgatatgg cgaagacgcc taattatatg
     5521 cgcttaaata tccacccggg ggagtggtat gtgggcgcga gataccacag tatttatact
     5581 gtgctgggct cgtgtgtatc cgtcaccgcg tggcaccctg aattaaaact aggcggcatg
     5641 tgccatttta ttctgccaac actgccgcgc ggtttaaaag atataaaagt taatgctggg
     5701 cgctacgcga atactgccct ggagatgatg aaactggcgc tgtcagcaca ggcgccactg
     5761 actgaatttc agctgggtct atttggcggc agcgatacca ttactcattt tgatgtaggt
     5821 aaaaataatg tcatttatgc gcaacaatgg ttggccgctg agcagctaaa cgctaaccga
     5881 atagacatag gtggccgtac ctcgcgctcg gtcacattgc atataagctc aggtgtgttg
     5941 attgtgaaac accacaatat gccaattatt aatcccctgc ccgctggcat taacacaaag
     6001 agactttaac tatgggcata gaagtgttag tggtagacga ctccgctgtg atgcggcaag
     6061 tgttgcggga aattctcaat gaggcgccgg atattaatgt gtatgcagcg gcgccggacc
     6121 caatatttgc cctacaaaaa atgcagggcc aatggccgga cgttatcgtc ttggatatag
     6181 aaatgccgcg catggatggt ttgacatttt tacgtaaaat tatggcagag cgccctacgc
     6241 ctataattat ctgttcttcg ctggcaaaaa aaggtaccga attatcgctg caagccctgg
     6301 ccgcgggcgc catagacgta attaccaaac cacaactggg cgtaaaagat tttctgctca
     6361 ccgctaaaca attattgtgg actactgtgc gcggtgcggc cacagcccgc atggcccggc
     6421 acagagtgga gcgcgaacct gtagccgtgg cacagccgcc cgtcgtagct gatttagtgg
     6481 cattaaccag cactacagat cgcataaccg taataggtac atctaccggg ggcacccaag
     6541 cgctagaaga aatattagtg agtgtgccgc gcacggcacc cggtattgca gttgtgcagc
     6601 acatgccgga attatttacc gccgcatttg cccagcgttt aaacagtatt tgcacggtgg
     6661 aagtaaaaga ggcaagttcg ggggataggt taattccggg ccgggtgctg atagctcccg
     6721 gcggccacca tttagaaatt caacgctctg gtgcgcaata tattgcaaaa gtatttcgcg
     6781 gcccggcggt aaatcgccac tgcccatcag tggacgtgtt atttcgctct gcggcagtgg
     6841 ccgctgggcg caacgccacc ggtattatta tgacgggcat gggcgatgac ggcgcccgcg
     6901 gtttattgga catgcggcag gctggcgccc gcaccatcgc acaagacgag gcatcctgcg
     6961 tggtatttgg tatgcctaaa gaagctatta acctcggtgc cgccaaagag accctatccc
     7021 tatctaaaat tgctaaggtg ttagagagct gactatgaaa acatctttag ctgaactaca
     7081 catattaatt gccgacgaca gcaacaccca gcggcagttt gcccagatgc attgcaataa
     7141 cttgggcatc gaacatattt atttggccgc cgatggtatg gagtccctgc atatattgca
     7201 gaacaatgcg attgatgcgg ttttaattga tttagaaatg cctattatgg atggcgttga
     7261 acttattcat gccatagctc agcataaact ctcgccctat attattatcg taagcgccaa
     7321 ggatcctatt ttaatcgcaa gtgttggcac catggcggaa gctgaggggc taagagtgct
     7381 gggcactttt aaaaaacccc tgcaaacaga gatgctgcag tgtagctttt tacatttatt
     7441 aaccgccctg caggcactga attacgataa tttagtgcct gcacctaatc ctgctcagat
     7501 ggccgtaacc ggcctggaac tcagccaggc aattaacaat aacgaaattt ttttagccta
     7561 ccagcccaag ctaagtgtgc aaggcctact gttgtgtggc gtagaggcgc tggcgcgctg
     7621 gcaacaccct gaaaaaggct taattgcccc agtggttttt attcctatgg ccgagcgctt
     7681 aggcctgatt agcgcactga cgcaaaagtt atttgttcta gccctagagg agaaacgcaa
     7741 atggcagcgg atgggttttc attttcatct ggcatttaat ttatcgcctt attctgtatc
     7801 cgacgaaggt ttggccgatt ggattgttgc cacagccaaa caatacgatg tgccgccgca
     7861 ggaaattatc ttcgagatta ccgaaaatgc tttgttaaat gaattagcct gcgccatacg
     7921 cacccttgct cgtttgcgtt taaaaggttt tagtattgcc attgatgact atgggacggg
     7981 ctttgccaat gcccaacaat tatcacgcgt gcccgccacg gaattaaaac tggaccgctc
     8041 gctggtgcac aacgtatccc agcgcccaca acagttgacg attttatcaa gctcgattga
     8101 gctgggtaaa aagttgaatt taaccttggt tgccgaaggt gttgaaaacc gcgatgatta
     8161 taatgtgttg gcgaatttag gggttgacca ggtgcagggc tattatttct ctcgcccttt
     8221 atacccggac gacctgctaa attttgttaa aacccgcctg agcgaaatcc gccgggcgac
     8281 acaataatgg gcgctcttta attgctgtaa tattttattg tcctctgtca ctttgcatcc
     8341 agagctaacc tgacgctgct acttttattc catttttaat ccaggggcaa atgattatcc
     8401 tgcaccgggg atttgagtac aaacgaggaa tgcaccccgg taatgccctc aatcgtggta
     8461 agtttattca gcaaaaaatg ctggtaggca tccatgtctt tcacaatcac ttttaataaa
     8521 tagtccgcct gctggccggt aattaaatgg cactcgagca cctctggcaa tttagtcagc
     8581 tttttttcaa acgccgcaaa gcgctctggc gtgtggcggt ccaggctaat ttgaataaag
     8641 gcggtgaggg ttaggcccag tttgcggcca tttaaacggg cgccgtagct gtcgataaaa
     8701 ccggcttctt ccaatgcccg caggcggcgc aggcaggggg aaggggagag gccgatggcg
     8761 tcggccaact cctggttgct gatgcggccc tggcgctgca gggtggctaa tatggcttta
     8821 tcaaatttat ccatggcaga ttattgctca taatgtatta ttgaggattt atactgccaa
     8881 atctacgccg attcaagccg aataagcaag ttaaatgcgc ggccgtgccg ctatactctt
     8941 cttaacctaa ttgaggagct agcgccatgt taaaaaaccc agctgaaaaa tatcgcccgt
     9001 ttaaaggtgt ggccttaccc gatcgcactt ggcccaaccg acagattacg cgcgcgccta
     9061 tttggatgag cacggatctg cgcgatggca accaagcctt aatggaccct atgaatgtaa
     9121 cgactaaatt gcgttttttt aagcatttgg tggctatggg ttttaaagaa attgaaattg
     9181 gttttccatc ggcatcgcaa atcgactggg attttgcccg cctgttaatt gagggcaagc
     9241 atattcccca agacgtaacg attgaggtat taacacaagc gcgcttggat ttaattgaaa
     9301 aaaccgtagc ctctgtggcg ggggctaagc aggtgattct gcatatgtac aatccggtgg
     9361 cgcccgcctt ccgccgcatt gtgtacaaca cggatcaagc aggggtgaaa gccattgccg
     9421 agcagggcac tcgttgggtg aaagagatta ccgcccgtta cccgcaaacc cagtggacgt
     9481 atcaatattc gccggaggta ttttctggta ctgaaattga atttgccaaa gaagtgtgcg
     9541 acgcggtgag tgcaatttgg cagcctacgc ccgagcgcaa aatgattatc aatttgcccg
     9601 ctacggtgga aatgggcacg cccaatacct acgcggatca aattgaatgg atgcaccggt
     9661 atttagacaa tcgcgccagt attattttga gcgtgcaccc gcataatgac cgcggcacgg
     9721 cggtggcagc ggcggaatta gccgttatgg cgggagcaga ccgggtagaa ggctgtttat
     9781 ttggcaatgg ggagcgcacc ggcaatgtgg atttggtgac cctggcgatg aatttatact
     9841 cccaggggat tcatccaggg ttggattttt ccaatatgga tcaggtgcgg caattagtag
     9901 aagaatgcac acaattgccg gtgcatccgc gccatccgta cgccggggaa ttggtgttta
     9961 cggcattttc cggctcccat caagatgcca ttaaaaaagg ctttgcccag caacagcctg
    10021 gcagtgtgtg ggaagtgccc tatttgccca tcgacccaca ggatgtgggc aagagttacg
    10081 atgcggtagt gcgggtgaat agccaatcag gcaagggcgg cgtgagtttt ttattgcagc
    10141 aggaagcggg ttttaattta ccgcgccgga tgcaaattga atttagcgct attgtgcagc
    10201 gggtgagtga taacaccggc cgggaagtga aaagtagcga tatagtggct ttgtttgagc
    10261 aggaatattt tgctcaaacc aaaccgtttg tgtatcacta caatgaaatt aaagcggtga
    10321 atgccagtga tgaccctgtg cgggtggata ttcacggcgc ttttcgccaa gaagccgtgg
    10381 tttatagcgg taccggcaat ggccctatag atgccgccgc caatgcttta agccaattta
    10441 ccggccagag tattcaggtg gtggattacc atgaacacgc cttaaccact ggctcacaaa
    10501 gccaggcggt gtgttatttg gaggtaaaag tgggcggtga taaggcattt tttggtgtgg
    10561 gcgtggataa aaatattatt gctgcggcga ttaaggcttt gtttaatggt gtgaatcgca
    10621 agcagaaata tagccaggcc tgttgatgcc agttaaatag acactgctga tttctctggt
    10681 tggtcacctg tgtgtgcatt tcaatgggat cacaccgttt ggaacggttt atcccggttt
    10741 tgcacggcgc cgcgataact aaaaactttt
//
LOCUS       DAOQGS010000069        10600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2923_length_10600_cov_4.979896, whole genome shotgun sequence.
ACCESSION   DAOQGS010000069 DAOQGS010000000
VERSION     DAOQGS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_10600_cov_4.979896"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKZ52_04045"
     CDS             complement(<1..727)
                     /locus_tag="PKZ52_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNG58974.1"
                     /translation="MTIFEFVAENMAIGIWGVLMLPLLILWRAGSACTWPLAKSVGGI
                     NLLLTLALWIAALQGPLVNGVLRLGFLQATLLVLVSFLGWIIYRYAASNFRGDSDGPR
                     FLRWLTFTLLAVQTLILVDHLVLFWTAWVAVSLSLHQLLLFYPNRPRALLAAHKKFLI
                     ARTAEALLALSIGLIYSHFGSFSLSFTMAAIAQIPAADVPWQLMVAGVCLALVALLKC
                     AQLPLHGWLIQVVESPTPVSALLH"
     gene            839..1720
                     /locus_tag="PKZ52_04050"
     CDS             839..1720
                     /locus_tag="PKZ52_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG58975.1"
                     /translation="MSKLNYHHLYYFWRVAKLGNLTKTAVNLHISQSALSAQIRNLES
                     TMGVELFIRDGRQLRLTDSGQSAYVYAEEIFKRGEELESLLSKGIQPDLLTVRIGVLT
                     TMSRNFVESFIEPLIRRPNTKYILHARGQVNLLNAVANHEFDLALTNIQVLGTNKELW
                     QCQLLARQPVAIVGPPDLPLGKVFSNRYREHEWVAPVTESPVRSAFDSFCAQHQFKPR
                     IVAEADDMAMLRLLARDTGALAVMPGVVVKDELQQGKLVNYLTLPNVYENFYAVTVKR
                     QIQNQLISELINNAIKL"
     gene            1734..2846
                     /locus_tag="PKZ52_04055"
     CDS             1734..2846
                     /locus_tag="PKZ52_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HNG58976.1"
                     /translation="MQNPTPNPNLLSRCITWLPHLAIALIVVSLALAFAWVAGWLTPD
                     QLTPALFTNAIETSNGQVHRGFRRAHAKGVCVAGYFEGNGQGVELSSARLFAPVRTPF
                     IGRMSIGGGSPYGLDNKARVRSMALQLNSDDGQQWRMAMNSFPFFAVSSVEAFYEQTA
                     ANAPDPATGKPDPAKVAAFAAAHPEFARFAEWAKSTPWSTSWANTTYNSVNTFRLSNP
                     AGEQHHVRWSMQPQTAFEAMSPEEREAADDNFLTEDLMARLAHEPLRWDMVVTIAAPG
                     DSITDPSKTWPEDRRKVTVGSVVIESSSPQTTGACRDINYDPLILPTGVDGSEDPLLA
                     ARSAVYSVSFNRRQHDIATGKADAAIGQASSQETSP"
     gene            2843..3484
                     /locus_tag="PKZ52_04060"
     CDS             2843..3484
                     /locus_tag="PKZ52_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNG58977.1"
                     /translation="MNDTVNVTENHDSLNHAESRPDPRHFNLTARVLHWSMALMILAM
                     LFVGIGMVTSLGLRPTLISIHQPLGIAILLLVIVRLINRLRHGTPPLPADLPTVQVFA
                     AKASHWLLYTLMFAMPLVGWSMLSAGGYPVTMFSGFVLPAIAPHNPTIYAVLRSAHTW
                     LALLLFATVLMHLAAALFHAWVRRDDVFASMARGDLGSKASVDTNDYDNSINR"
     gene            complement(3773..4699)
                     /locus_tag="PKZ52_04065"
     CDS             complement(3773..4699)
                     /locus_tag="PKZ52_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HNG58978.1"
                     /translation="MIKKRSKLSRRDLDFIDKSIEISDSPASIENATFMFLPFCQLGF
                     PRSSTEEVFFTRSCGKMEIKIHDGTNGTISKIPFGSAARLILAYVCSHAVRFKTPAIE
                     LGNSAAEFMRKIGLTSSGGKKGSLTSVKSQLFNIHVCHIEVKYANGKKIIIFDGPIFR
                     DCLAVANFENGWRTHLHLNENFYEVLVEQKNCLPIDSRAVTALKRSALAMDIYFMLAE
                     RLHRAKQKPSILYWKNLRDQFAHEYADNDNGKRSFRRNFIAALRKVLMVYPGAKVKLC
                     SGGIEIQKSPPPIPNKKLGSTKAQFVDKPSID"
     gene            complement(4696..5712)
                     /locus_tag="PKZ52_04070"
     CDS             complement(4696..5712)
                     /locus_tag="PKZ52_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58979.1"
                     /translation="MNKYSHLDQSTKLAIDSFLKTAIKMAKRFDKSNPSEDTRNEYMK
                     IAKQLLLKKAKNKQSLWQAIADTRSKNTYYKRIAAISFYILQIGSESTKTLMNEYCPI
                     ADNNIKKAAVDVMELFNIIETSLKITFSKRLSKRSALLGLPSDWMEQICNYNENSKYR
                     DAFYVAALTGCRPSELKKGIVATYKLSSDGESELTFKIFGSKVTADSGQEWRTITYRN
                     LEIIGAFSKFLASIKAEVTKRIHIESPINFTQEVRRIAKHLWPDHKSPVTAYCFRHQF
                     SANLKSLKIGDDTSKALGHRSTKTRRLYGTASQARGRPLEISISSSHDLKQHPAKGND
                     GVEP"
     gene            complement(5705..5929)
                     /locus_tag="PKZ52_04075"
     CDS             complement(5705..5929)
                     /locus_tag="PKZ52_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HNG58980.1"
                     /translation="MNAKTQIIRPAALAKQLNVSLTTIWRWRLQGIIPQPVVLGSRII
                     GWDQSTIDEWLQKLKDKIEKSDKKGGGKNE"
     gene            5988..7226
                     /locus_tag="PKZ52_04080"
     CDS             5988..7226
                     /locus_tag="PKZ52_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58981.1"
                     /translation="MNIFDVIKTLLILLAGINMLDYNQLELEIDKVEKKLKSLRKRAI
                     KDGQSLLLEEKVGEIFVGDAIRRHPIDNAMILHLMQDLKILQKCKSKPPKYLLDVVAI
                     VGGFKELLLEIHRITIYRQTLNNEEYKNYLMHLSEVLQLCKTYAFKRTAKIIHSTLEC
                     CLCWRAVKPGSTQYCVAHQEDKMLRQRDQRRLYAAIMSRNDEHSAELKRRLASKSGRK
                     RLHFRKHYWSSFFAEDLTPFQLKKNTKISIPSGKEWREDAQLFLDYLKDRYIYWNEYI
                     RRVSPRDSKDWVSWLGKIRKRLDPKCPPVFTAKDIESTEEYPVNIWLCPRGDIEDWAL
                     LLRIVQRFESYHVLHSAPKKRGPQIGTVPKNDLLREKIKKIANKQIKKIGKIKAVAIA
                     DELSISPQRVSVLLKELGLR"
     gene            complement(7231..8463)
                     /locus_tag="PKZ52_04085"
     CDS             complement(7231..8463)
                     /locus_tag="PKZ52_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNG58982.1"
                     /translation="MNDAQLKAKLKSGQPGKFTVDRGLYFRVSEEGNGFWMLRYSIND
                     KRREFVFARYGRPPEGMTLAEARLESARLRSLVNQGIDPAAEKKRSQLITLKTVNDVA
                     HDWIGECRKRLENPHIPERVYRNDISPAIGELNIDRVTALDVLGIIRKINESGRPTIA
                     NDALHYCKQIFNHGIKLGLLQTNPSLAFSIKDAGGIEQSRDRALTVDELRVVFRVFRE
                     HADVFTRENYLSMAILVILGVRKGELIAATWDEFDFTAKTWHLPPGRTKTSAEIIIPL
                     PDQVITWLHELKIRSVNSAYVFPARRASKRREYISDDTLNHALAKLFGLKVDAKKKPL
                     PNYLGEAGIEHFVVHDLRRTCRSLLAANGIPSHIAERCLNHKLRGVEGIYDRYDYMDE
                     RRKALAIIADQLAPLVEA"
     gene            complement(8643..9503)
                     /locus_tag="PKZ52_04090"
     CDS             complement(8643..9503)
                     /locus_tag="PKZ52_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNG58983.1"
                     /translation="MPRSMTGFARIEEQQPWGQIICEIRSVNHRYLEPSIRMPDLLRR
                     LEIPLRDQLRSQLNRGKVEASIFIKWDDSANQAVNLDAARNLIDLAQSLSQHILHPAP
                     INPLEVLRWPGVLQTPSLDQDALDTAVLNVFNRTLSELQANRSREGAELAQLIEQRLV
                     QIQTGVNQVKEFLPKLQQTQQEKLTAKLAALAVEIDRERLAQELVYLAQKTDVAEELD
                     RLETHLIEVRHCLKQKEPVGRKLDFLMQELNREANTLSSKSSASETTQIAVELKVLIE
                     QMREQVQNIE"
     gene            9790..10512
                     /gene="rph"
                     /locus_tag="PKZ52_04095"
     CDS             9790..10512
                     /gene="rph"
                     /locus_tag="PKZ52_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNG58984.1"
                     /translation="MQRPSGRASHQLRPISITRNYTKHAEGSVLVCFGDTQVLCNVSV
                     TEGVPRFLKGTGQGWLTAEYGMLPRSTGSRMDREAARGKQGGRTLEIQRLIGRALRAA
                     VDLKKLGEYTLVVDCDVIQADGGTRTASITGACVAMVDALNFLQRKGQLKTDPLLGIV
                     AAVSVGVYQGEPVLDLDYLEDSAADTDLNLVMNEAGGIIEIQGTAEHGAFSEAQLHSM
                     LSLGKAGIADLIELQKQALAEG"
BASE COUNT         2638 a         2430 c         2611 g         2921 t
ORIGIN      
        1 cgtgcagcaa tgccgatacc ggcgtaggtg actccaccac ctgaatcaac cagccatgca
       61 ggggcaactg ggcgcattta agtagggcaa ctaatgccaa acaaacaccg gccaccatga
      121 gttgccaagg cacatccgcc gcagggattt gcgcaatcgc tgccatggtg aaagacaacg
      181 agaagctgcc aaagtggctg tagataagcc cgatgctgag tgccagtaac gcctcggcag
      241 tgcgtgcaat caggaacttt ttgtgtgccg ccagcagtgc acgcgggcga ttgggataaa
      301 acagcagtag ttgatgcaag cttaggctca ccgctaccca agcggtccag aagagcacca
      361 ggtgatcgac cagaattaag gtctggaccg ccagcagcgt aaaggtgagc cagcgcagga
      421 agcgaggtcc atcgctatcg ccgcgaaaat tactggctgc atagcggtag ataatccagc
      481 ctaaaaagct caccagtacc aacagggtgg cctgcaaaaa accgaggcgc agcaccccgt
      541 taaccaaagg gccttgcaac gccgctatcc acaaagcgag cgtcaataac aggttgatgc
      601 ctcccacgga ttttgccagt ggccaagtgc aggcgctgcc cgcgcgccac aaaatcaaca
      661 gtggcaacat gagcacgccc caaattccta ttgccatgtt ctcggcgaca aactcgaata
      721 tcgtcatgtc ttccaccttc tttttgtgta ggtgggggca ttctgggcta gcctaatcca
      781 taggtaaaat acataattat cgacatatca ttcacttaaa tagaacgatt ggcgcgctat
      841 gagcaaactt aactaccatc acctgtacta tttttggcgc gtagccaagt tgggcaacct
      901 gaccaagact gccgtcaatt tgcatatctc tcaatccgca ctctcggcgc agattcgcaa
      961 tctggaatcg actatgggtg ttgagctatt tatccgcgac ggtcgccaat tgcgcctgac
     1021 tgacagtggg cagagtgcgt atgtatatgc cgaggagatt ttcaaacgcg gcgaggagct
     1081 ggaatcgctt ttgagcaagg gaatacagcc ggatttgctc accgtaagaa ttggtgtgtt
     1141 aacgactatg tcgcggaact ttgtggagag ttttattgag ccgctgattc gccgtccaaa
     1201 tacaaaatat attttgcatg cgcgcggcca agtcaatttg cttaacgcgg ttgccaacca
     1261 cgagttcgat ctcgcgctca ccaatattca ggtgctggga accaataagg aattgtggca
     1321 atgccaatta cttgcgcgtc agccggtggc gattgtcggc ccgccggact tgcccttggg
     1381 aaaagtattt tccaatcgct acagggagca cgagtgggta gcaccggtga ccgaaagccc
     1441 ggtccgctca gcattcgata gcttttgtgc acaacaccag ttcaagccac gcattgtcgc
     1501 cgaggctgac gacatggcta tgctgcggct actcgctcgc gatacaggcg ccctagcagt
     1561 gatgcccggc gttgtagtga aagatgaatt acagcaaggt aagttagtga attatctaac
     1621 cttgcccaac gtatatgaaa atttttatgc agtaacggta aagcgacaaa tacaaaatca
     1681 gttgataagt gagctgatta ataatgcaat caagctgtag atgagaaacg tccatgcaaa
     1741 accctacacc caaccctaat ctattatcgc ggtgcataac ctggcttcca caccttgcta
     1801 ttgcactcat cgttgtctct ctcgcgctcg cgtttgcgtg ggtggctggg tggcttacgc
     1861 cagaccagct taccccggct cttttcacca atgcgattga gaccagcaac ggacaagtcc
     1921 atcgcggttt ccgtcgcgcg catgccaaag gcgtgtgcgt ggcaggttat tttgagggta
     1981 atggccaggg cgttgagctc tccagcgcgc gcctgttcgc gccggtacgc acaccgttta
     2041 tcggacgcat gtcgattggc ggcggctcgc cctacgggtt ggacaacaag gcgcgcgtgc
     2101 gcagtatggc actccaactc aatagcgacg acggccagca atggcgcatg gcgatgaata
     2161 gctttccatt ttttgcggta tcgtcggttg aggcgttcta cgagcaaaca gcggccaatg
     2221 cgcccgatcc tgcaacaggc aagcccgacc ccgctaaagt ggccgcgttt gcagcggcac
     2281 atccggagtt cgcgcgattc gcggagtggg ccaagagcac gccttggtca accagttggg
     2341 cgaatactac atacaacagc gtcaacacct ttcgcttgag caaccctgcc ggtgagcagc
     2401 atcatgtgcg ctggtcaatg cagccgcaga cagccttcga ggctatgtcg cctgaagagc
     2461 gagaggctgc cgacgataac tttcttacag aagatctcat ggcgcgactc gcgcatgaac
     2521 ccttgcgctg ggacatggtg gtgacaatag ctgcaccggg tgattcgatt acagatccat
     2581 cgaaaacctg gccggaagat cgccggaaag tgacagtggg cagtgtggtg atcgagagtt
     2641 catcgccaca aactaccggc gcgtgccgcg atattaatta cgatccgcta atactgccaa
     2701 cgggcgtaga tggctctgaa gatccattgc tggcagcacg ctccgctgtc tattcggtct
     2761 cattcaatcg tcgccagcat gatatagcta ccggcaaagc tgacgctgcc atcggtcagg
     2821 cctcttcaca ggagacttct ccatgaatga caccgtgaac gtgactgaga atcatgattc
     2881 cctaaaccac gcagagtctc gtcctgatcc gcgccatttc aaccttacag cgcgcgttct
     2941 gcactggagt atggcactaa tgattttggc gatgttgttc gttggcatcg gcatggtcac
     3001 gtcgctcggt ttgcgtccta cgctaatcag catccatcaa ccgcttggca tcgctatttt
     3061 gttgttagtg attgtgcgat tgatcaatcg cctgcgccac ggcacgccgc cattgcctgc
     3121 cgatctgccc accgtgcagg tgttcgcggc caaggcttca cactggttgc tgtacacgct
     3181 gatgtttgcc atgccgttgg ttggctggtc aatgctgtcg gcaggcggtt atccggtgac
     3241 gatgttcagt ggtttcgtac taccagccat cgcgccccat aaccccacca tatatgccgt
     3301 attgcgcagc gctcatactt ggctggcgct gctgctattt gccacggttc tgatgcattt
     3361 ggcggcggcg ttgttccacg cttgggtgcg ccgcgatgac gtgtttgcaa gcatggcacg
     3421 aggagacctt ggttccaagg catcagtgga cacaaatgat tatgacaatt caatcaatag
     3481 gtgatgccct gtgacacaaa tagggaaaga gacttcttga aactaattat gttttgggtg
     3541 tgatgcgaaa cgatgtagtg aaagatgaat tgcagcaagt ctggtcgtcg aattttttat
     3601 taacaaataa tcatctagat ataggttgta tataggtaaa caaatttata ggtttgttta
     3661 caggtagtgg cgggttccta attctgcgta aagtttcttc tagttctgcg cgaacaacgt
     3721 cggagaaaat ccttcttctg cgacccgtcg taattaaatt tgcgtgaatg gatcaatcaa
     3781 tacttggctt atccacaaat tgcgctttag ttgagcctag ttttttattg gggataggcg
     3841 gcggtgattt ttgtatctct ataccacctg agcacaattt cacttttgca ccggggtaaa
     3901 ccatcagtac tttgcgaagc gcggcaatga aatttcgtct aaaactccgt ttaccgttat
     3961 cgttatctgc atattcatga gcgaattgat cacgcagatt tttccaataa agtatcgatg
     4021 gtttttgttt ggcacgatgt aatctttcag caagcataaa atatatatcc atagctaagg
     4081 cggaccgctt tagagcagtt actgctcggg aatcaattgg caggcagttt ttttgctcca
     4141 caagaacttc ataaaaattt tcatttagat gtagatgagt tctccagccg ttctcaaaat
     4201 ttgcaacagc tagacagtcc ctgaaaattg gaccatcgaa aattatgatc tttttaccgt
     4261 ttgcatattt aacttcaata tggcaaacat gaatattaaa gagctgtgac tttacgcttg
     4321 tcaggctgcc ttttttgccg cccgatgatg ttagcccaat ttttctcatg aattcggcgg
     4381 cgctatttcc caattctata gctggtgttt tgaatcgaac ggcatgacta caaacataag
     4441 ccagaattaa tcttgccgcg gatccaaacg gaattttaga gatagttccg tttgttccat
     4501 cgtgaatttt tatctccatt ttcccacagc ttcttgtaaa gaaaacctcc tcagttgagg
     4561 agcgcggaaa tcccaactgg caaaatggca aaaacataaa agttgcattt tctatcgaag
     4621 ctggcgaatc agagatttct attgatttat caataaaatc taaatctctt cttgataact
     4681 tagatctttt tttgatcatg gctctactcc gtcatttcct tttgcgggat gctgttttag
     4741 atcatgcgac gatgaaatac taatttctaa cggccttcct cttgcttgag atgctgtgcc
     4801 gtaaagccgc cgtgttttgg tagatctatg tccgagcgct ttgctagtat cgtcaccgat
     4861 tttcaaactt ttcaggtttg cggaaaactg atggcgaaaa caatatgcag tgactgggct
     4921 cttatgatca ggccaaagat gttttgctat tcgtcgtact tcttgtgtga aattgatcgg
     4981 gctttcaata tgtattctct tcgtcacttc tgcctttata gacgcaagaa acttcgagaa
     5041 agcccctatt atttcgaggt tgcgataggt tattgttcgc cattcttgac ccgagtccgc
     5101 agtaactttt gagccaaaaa ttttgaaggt tagctctgac tcaccatcag agctcaattt
     5161 atacgttgcc acgatccctt tttttagttc gctcgggcga caccccgtta atgcagcaac
     5221 ataaaatgca tctcgatatt ttgaattttc attgtaattg caaatttgtt ccatccaatc
     5281 gcttggcagt cccagtaacg cagatcgctt agatagtctt tttgaaaaag ttattttaag
     5341 gctagtttca ataatattga aaagctccat cacatcaacg gcggcttttt ttatgttgtt
     5401 atcagcgata gggcaatatt cgttcatcag tgtctttgtt gactctgaac caatctgcag
     5461 aatatagaag ctaatagccg caatccgttt gtaataggtg tttttgcttc gtgtatcagc
     5521 tatggcttgc cacaagcttt gcttgttttt tgcttttttt aaaagtagtt gctttgctat
     5581 cttcatgtat tcgttccgtg tgtcttccga tggattgctt ttgtcaaacc tttttgccat
     5641 ttttatggct gtttttaaaa atgaatcgat agctagtttc gttgattggt caagatgcga
     5701 atatttattc atttttacca cctccttttt tatcggattt ttcaattttg tctttcagct
     5761 tttgtagcca ttcgtcgatt gttgattgat cccaaccaat gatgcgtgaa cccagaacta
     5821 cgggttgtgg aatgatgccc tgtaagcgcc agcgccaaat agttgtgagt gatacgttaa
     5881 gctgtttagc cagagctgcc gggcgaataa tttgagtctt tgcgttcata ttgaatcctc
     5941 tgcatatgtg cgaaattgca tgagcattct tgatttgttc tgaattcatg aatatatttg
     6001 atgtaatcaa aactctattg attttattag cgggcataaa catgcttgat tacaaccaac
     6061 ttgaacttga aattgataaa gtcgaaaaga agctcaagtc ccttagaaaa agggctatca
     6121 aagatggtca gtcactgctg ttggaagaaa aggttggcga gatttttgtt ggtgatgcga
     6181 ttcgtcgcca tccaattgac aatgctatga tcttgcatct catgcaagat ttgaaaattt
     6241 tgcagaaatg taaaagcaag cccccaaaat atttactgga tgttgttgct atagttggag
     6301 ggttcaagga acttttgtta gagatccacc gaataaccat ctatcgccag acactgaaca
     6361 atgaagaata taaaaattat ttaatgcatc tgtctgaagt tctacagctc tgtaaaacct
     6421 atgcgtttaa gcgtactgcc aagatcattc attcaactct tgaatgttgt ttgtgttgga
     6481 gagcggtaaa accaggttct actcaatatt gcgtcgcgca ccaagaagat aagatgcttc
     6541 gtcagcgaga tcagcgtcgt ctgtatgctg caattatgtc ccgtaatgac gagcactctg
     6601 ctgagttaaa acgaaggctt gcatcaaaat ctggaagaaa aagattgcac tttagaaaac
     6661 attattggtc tagttttttt gccgaggatt tgactccttt tcagctgaaa aaaaatacta
     6721 aaatatcaat tccatcagga aaagagtggc gtgaggatgc tcaattattt ctcgattacc
     6781 ttaaagacag gtacatatat tggaatgaat atattcgtag agtttcgcca cgcgattcta
     6841 aagattgggt aagttggctg ggtaaaatcc gaaaacgatt agatccgaag tgtccgccgg
     6901 tattcacagc aaaggatatt gaatctacag aagagtatcc ggtgaatatt tggctttgcc
     6961 ccagaggcga cattgaagac tgggcactgc tgttaagaat agtgcaacgt tttgaatcct
     7021 atcacgtcct tcactctgca cctaaaaagc gaggccccca aattggcact gttccaaaaa
     7081 atgatttatt gcgagaaaaa attaagaaaa tagcaaataa acaaataaaa aagatcggga
     7141 aaataaaagc cgttgccatc gctgatgagc tctcaataag ccctcaacgc gtttcagtcc
     7201 ttctaaaaga gttaggcttg agatgacttg tcatgcctca accaatggtg caagttggtc
     7261 tgcaataatt gccagtgctt tgcgacgttc atccatgtaa tcatatcgat cataaatacc
     7321 ttccacgccg cgcaatttat gattaaggca acgttctgcg atgtgactcg gaatgccatt
     7381 cgcagccaga aggcttctac acgttctacg taagtcgtga acaacaaaat gttctattcc
     7441 tgcctcacca agataattcg gtagaggttt tttcttcgca tccactttca atccaaatag
     7501 ttttgccagc gcgtgattaa gagtgtcgtc agaaatatat tctctgcgct tgctcgcccg
     7561 ccgtgcagga aatacgtatg cagagtttac tgacctaatc ttgagctcat gcagccaagt
     7621 gatcacttgg tcgggtagcg ggataatgat ttcagcactt gtttttgtac gccctggagg
     7681 taggtgccaa gtttttgcag tgaaatcaaa ttcgtcccaa gttgcagcaa ttagctctcc
     7741 tttgcgcact cccaatatca ccaagatggc catggacaaa taattctcgc gcgtgaaaac
     7801 atcggcatgc tcccgaaaca cgcggaagac aactcttagt tcatcgactg ttagtgcgcg
     7861 atctcttgat tgctcaattc cgcctgcatc tttaatgcta aaagcaagtg acggatttgt
     7921 ttggagtagg cctaatttga tgccgtggtt aaaaatttgt ttgcaatagt gaagggcgtc
     7981 attggcgata gtcgggcggc cgctttcatt aatcttgcga ataattccga gcacatccaa
     8041 cgctgtgaca cgatcaatgt tgagctcgcc gatggcggga gaaatgtcat tgcgatacac
     8101 ccgctcaggt atgtgagggt tttccaggcg cttgcgacac tccccaatcc aatcgtgagc
     8161 gacatcatta actgttttga gtgtgatgag ctgagagcgc tttttttcgg cagcaggatc
     8221 tatgccctga ttgaccagtg agcgcaagcg tgcggactct aaccgagcct cggcaagtgt
     8281 catgccttct ggtgggcgac catagcgagc aaaaacgaat tcccgtcttt tgtcattgat
     8341 gctgtagcgc agcatccaga atccgttgcc ttcttcactt acacggaagt agagaccgcg
     8401 atcaactgtg aatttacctg gttgacctga tttaagtttg gcttttaatt gtgcgtcgtt
     8461 catggctttc agctaagtgg tggcaggttt ttcaagctta cacgaaactt tgaaatctgc
     8521 cacctattct gccaccacta acttgcaatc atgagaggta ttctgaaatc ttgtgaaatc
     8581 taaaaattaa ttaagttatt gatttgtatg taaaatttta cttcatgaaa ttgcatgaat
     8641 tatcactcaa tattctgcac ctgctcgcgc atctgttcga tcaacacttt taattccacg
     8701 gcaatttggg tggtttcact ggcgctggat ttagacgaga gcgtattggc ttcgcgattt
     8761 aattcctgca ttaaaaaatc caatttacgc cccaccggtt ctttttgttt taggcagtgg
     8821 cgcacttcga tgaggtgggt ttccaggcgg tctaattctt cggccacgtc ggttttttgc
     8881 gctaaataca ctaattcctg ggctaggcgc tcgcggtcta tttctacggc tagggcggcc
     8941 agtttagcgg tgagtttttc ctgttgggtt tgctggagtt tgggtaggaa ttcttttact
     9001 tggtttacgc cggtttgaat ctgcaccagg cgctgttcaa ttaattgggc tagttcagcg
     9061 ccttcgcggc tgcggttggc ctgcagctcg cttaaggtgc ggttaaatac atttaatacg
     9121 gcggtatcta gggcgtcttg gtccaggctt ggggtttgta atacaccggg ccagcgcaat
     9181 acttccagcg ggttaatggg ggcggggtgg agtatgtgtt ggcttaggga ttgggcgagg
     9241 tcaatcaggt tgcgggcggc gtctaggttc accgcttggt tggcgctgtc atcccattta
     9301 ataaaaattg aggcctccac tttgccgcga tttaattggc tgcgcagttg gtctcgcagg
     9361 gggatttcta agcgcctgag caaatccggc atacgaatgg agggctctaa atagcggtgg
     9421 ttaacgctgc ggatttcaca aataatttgg ccccagggct gttgttcttc gatgcgggca
     9481 aagccggtca tgctgcgcgg cataggcggg tctcttgggt taaattgtta ataggtttga
     9541 tgatcggtag cctggatgcg gcagaacccc ggatttcgct gcgctgcatc caggctgcga
     9601 gttgtataac cggcgcaaac cggttaaggc cgtcgcgccg gagtgagcgg ggccatgtgg
     9661 ggtggagtct agcattagac gctttgcccc agggtggtcc ggtcaatgtt aaacacggca
     9721 aattgagggc aaaacttcac cgccaactgg ctataatgcc cgcccatttg tttaaggctt
     9781 gaatgattca tgcaacgacc cagcggccgc gccagccacc aattgcgccc catcagcatc
     9841 actcgcaatt acaccaaaca cgctgaaggc tcggtgttgg tgtgttttgg cgatacccag
     9901 gtgctgtgta atgtctcggt aactgagggg gtgccgcgtt ttttaaaggg cacggggcag
     9961 ggctggttaa cggcggaata cggcatgttg cctcgctcta ccggcagccg tatggaccgc
    10021 gaagcggccc gtggcaaaca gggtggtcgc accctggaaa ttcagcgttt aattggccgc
    10081 gccctgcggg cggcggtgga tttaaaaaaa cttggtgaat acaccctggt ggtggattgt
    10141 gatgtgatcc aagccgacgg cggcacccgc acggcgtcaa tcaccggtgc ctgcgtggcc
    10201 atggtggatg cgttaaattt tttacagcgc aaaggccaat taaaaaccga cccattgctg
    10261 ggtattgtgg cggcggtgag tgtgggtgtg taccagggtg agccggtgtt ggatttagat
    10321 tatttagaag actccgccgc tgatacggat ttaaatttag tgatgaacga agcgggcggc
    10381 ataattgaaa ttcaaggtac ggcggagcac ggcgcgttta gtgaggcgca attgcacagt
    10441 atgttgagct tgggcaaggc gggaattgcg gatttaattg agctgcaaaa acaggcattg
    10501 gcggaaggtt aggtttataa aaaggcgggt taaactcgcc ttttttggct cttcgtagaa
    10561 ttgtgtaaaa cccccgcgcc agatggaaaa atggcctccc
//
LOCUS       DAOQGS010000070        10548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2945_length_10548_cov_3.680263, whole genome shotgun sequence.
ACCESSION   DAOQGS010000070 DAOQGS010000000
VERSION     DAOQGS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2945_length_10548_cov_3.680263"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="purB"
                     /locus_tag="PKZ52_04100"
     CDS             <1..1126
                     /gene="purB"
                     /locus_tag="PKZ52_04100"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNG58985.1"
                     /translation="TTNHDVKAVEYFIKEAFKGNSELEAINEFVHFACTSEDINNLSH
                     GLMLKAGRDQVLLPAMQEIVHAIAAMAKEYAAVPMLSRTHGQTASPTTVGKEMANVAY
                     RLARQITQIKQVQLLGKINGAVGNYNAHLSAYPDINWQAQAQHFIESLGLTFNPYTTQ
                     IEPHDYIAELFDVIARFNTILIDFNRDIWGYISLGYFKQKTIAGEVGSSTMPHKVNPI
                     DFENSEGNLGLANAVMLHMAAKLPISRWQRDLTDSTVLRNMGVGFGYCEIAYASALKG
                     IRKLEINTPVIAADLDSAWEVMAEPIQTVMRRFAIEEPYEKLKALTRGQKITQAIMQS
                     FVEQLDIPPTAKAQLKALTPSTYVGNAEQQALDLLAQLPQ"
     gene            1233..1307
                     /locus_tag="PKZ52_04105"
     tRNA            1233..1307
                     /locus_tag="PKZ52_04105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Arg,seq:cct)
     gene            1360..2073
                     /locus_tag="PKZ52_04110"
     CDS             1360..2073
                     /locus_tag="PKZ52_04110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNG58986.1"
                     /translation="MNILLLNPDDLNPTGTVTLRGPKLEHLNNVLKVIPGKLINVGLK
                     NSWLDQAVVEAINSEFVQLRLLGNQQPPPPALPCSLILGLPRPRMLQRSLQTIATMGV
                     EKLLLIASERVEKSFWQTPLLKPAAIQEQLEIGLEQAKATQVPGVILCPNWREFAHTL
                     LPALSSQCLNKIIAHPGDAPRATGLADGQSLVAVGPEGGFVDSEVALFKELGFSQVQL
                     GARILRVETAIPVLLAKLF"
     gene            complement(2172..3851)
                     /gene="rpsA"
                     /locus_tag="PKZ52_04115"
     CDS             complement(2172..3851)
                     /gene="rpsA"
                     /locus_tag="PKZ52_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNG58987.1"
                     /translation="MSESFAELFEESLKTVDMVPGSIVTGVVIDIDSDWVTVHAGLKS
                     EGVIPAEQFYDENGKLTLSIGDEVQVALETVEDGFGETRLSREKAKRAESWKVLESAH
                     AAEEVVKGIINGKVKGGFTVDVAGIRAFLPGSLVDVRPVRETTHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVMEQANSDERDELLATLQEGMAVKGVVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRIKHPSEIVTVGDEIEVKVLKFDRERNRVSLGLKQLGDDPWAAITQRYPE
                     GAKVKARITNLTDYGCFAEIETGVEGLVHVSEMDWTNKNIHPSKVVQLGDEVEVMILD
                     IDEERRRISLGIKQCQENPWDAFSKQFAKGDKISGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNETGEEAVRKFKKGDELETVVLAIDPERERISLGIKQLEQDPFTVYVGTYDK
                     GSIVKGTVKEVDAKGAVIVLADDVEGTLKASDLSREKVEDARNVLKEGDVVEAKITNV
                     DRKNRTIILSVKAKDVDDEKSSIKDLKQSEQVAPATIGDLIKAQIESQQNN"
     gene            complement(4101..4385)
                     /locus_tag="PKZ52_04120"
     CDS             complement(4101..4385)
                     /locus_tag="PKZ52_04120"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HNG58988.1"
                     /translation="MLRFLVWLKRLFVLLWLLVLVFLSAAITADNPQLITLHLFGKVY
                     EASAGFIFMVSLGVGALLGIILLLPRLWLLQRKVRKLLKRLEQANDHPAR"
     gene            complement(4385..5053)
                     /gene="cmk"
                     /locus_tag="PKZ52_04125"
     CDS             complement(4385..5053)
                     /gene="cmk"
                     /locus_tag="PKZ52_04125"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNG58989.1"
                     /translation="MIPVITIDGPSGAGKGTVSRLVAERLGFTILDSGALYRLTALAA
                     MGQGVDLADQHGLAALARTLDIEFSLRNGQVVILLAGREVTDAIRAEDVGMGASKVAA
                     LPAVREALLQRQRDFRQLPGLIADGRDMGTVVFPDAPLKIFLTASPAVRAKRRYDQLQ
                     QQGLTADLEQITADIEKRDAQDAQRAVAPLRPADDAKLLDSTALSIQEVLDAILQYWR
                     EVQA"
     gene            complement(5215..5919)
                     /gene="pyrF"
                     /locus_tag="PKZ52_04130"
     CDS             complement(5215..5919)
                     /gene="pyrF"
                     /locus_tag="PKZ52_04130"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNG58990.1"
                     /translation="MSVASPLIVALDYADAKAAYALAERLDPRYCRVKVGKELFTAAG
                     PQAVETLQAKGFECFLDLKFHDIPATVAKAVAAAAQLGVWMVNVHASGGLPMMQEAKA
                     ALGKFSSPPLLIAVTVLTSMSQEVFAQTGFAGAIADRVSFLARLAAEAGLDGVVCSAL
                     EARAIKQQAGAALLAVTPGIRPLGADNQDQSRVLTPSAALEAGSDYLVVGRPITQAAD
                     PAQACYELYQSLNLDA"
     gene            complement(6000..7862)
                     /locus_tag="PKZ52_04135"
     CDS             complement(6000..7862)
                     /locus_tag="PKZ52_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNG58991.1"
                     /translation="MIQDLRENLKGTVAVVIGAVVLLAMIVTGQEVSKTSFTDTVASV
                     NGDDISLKDLRRAMTQEKIRLKNQFGMPDDAEQLKDENLKQPALTSLMRQRAVIQAAQ
                     RAGMGVSEVVVKDQIKQAFTQDKQFNAQGFNAYLANFGFTQATLLQQESETYIVRQLL
                     SGLSDTAFITQQDVELLAGIVGQKRSFSAVTIPKEKAAKVTPTEEEIAQYYQDNQSSF
                     SEPEKVSLQYLEVSVAQLAKSQAVTEEEIKAAFDKELAEFKSEPELSIAHILIEAGKN
                     SAESDAKIAQVQAKINAGEAFDALAKTYSDDLGSKELGGDLGVMTGDSFPAEFKTAVA
                     ALKEGQVSGPLKSSAGTHFVKLVKKTQLVPPTLEGRRSALAQQLATDKAAEKFVDQIK
                     SLEEATFGKSDLASAATALGLNIQETELFAKTGAAGITSEPLVLEAAFAKDVLEQGQI
                     SKVLELPQERAVVVRLKEHKPSHVKALAEVRGAIVETLVQQKTAAQLQQLAKALSDKL
                     RAGAKPEDAAKELGYTFAQFEKQDRFKSTADREILQYAFGMVRPAAQPQVENFATRSG
                     GQVVISLTAATNGTVADLEAQQLAGIKSQVVQQKSTDDIAAYEGAHFSAAKVKM"
     gene            complement(7963..8277)
                     /locus_tag="PKZ52_04140"
     CDS             complement(7963..8277)
                     /locus_tag="PKZ52_04140"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNG58992.1"
                     /translation="MFNHYVPNNKRGCYVNKSELIDAIAASADISKAAASRALDAVTE
                     SIMGALKKGDQVALVGFGTFAVKERAARTGRNPQTGAPIDIAAAKIPSFKPGKALKDA
                     VN"
     gene            complement(8423..>10548)
                     /gene="lon"
                     /locus_tag="PKZ52_04145"
     CDS             complement(8423..>10548)
                     /gene="lon"
                     /locus_tag="PKZ52_04145"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNG58993.1"
                     /translation="RAQIESVDESERCFKAHCTTIPADKVYTREEETMARGLLKRFEH
                     YVNFSKKVPVEVLNSLNGIDEPGRLVDTIAAHMTLDLGQKQRVLELFKVGERLEHLIG
                     LMDAEADLYQVEKKIRGRVKKQMEKSQREYYLNEQLKAIQKELGELGEDAGEGDEFER
                     RILDAKMPKAAEDKTRAEINKLKMMSPMSAEASVIRSYVDWMLNVPWNKRSKINMDLA
                     HAEKVLEEDHYGLTEVKERILEYLAVQKRVKKIKGPVICLVGPPGVGKTSLGESIARA
                     TNRKFVRMALGGVRDEAEIRGHRRTYIGSLPGKLLQKIAKTGVKNPLFLFDEIDKMGM
                     DHRGDPASALLEVLDPEQNNKFNDHYLEVDYDLSDVMFVCTSNTMNIPGPLLDRMEVI
                     RIPGYTEDEKLNIAMRYLLPKQRKNCGLRDDELKVEESALRDIIRFYTREAGVRSLDR
                     EIAKLCRKVVTENVKSKKSAPRVLTSEHLEHYLGVKKTDYGRADTEDRIGQVTGLAWT
                     QVGGELLTIEIASVRGKGRLVKTGSLGDVMQESIQAAFTVVRSRAKVLGIAPDATEKH
                     DIHIHVPEGATPKDGPSAGIAMCTALVSALTRIPVRADVAMTGEITLRGQVLKIGGLK
                     EKLLAAHRGGIRTVLIPGDNEADLREIPDNIKADLIIKPVRHIEEVLAVALTHAPAGF
                     SDDEFAALESKAFDLPKDSPGISTH"
BASE COUNT         2597 a         2839 c         2476 g         2636 t
ORIGIN      
        1 aacaaccaac cacgatgtaa aagcggtgga atattttatt aaagaagctt ttaagggcaa
       61 tagcgaatta gaagccatca atgagtttgt gcactttgcc tgcaccagcg aagatatcaa
      121 taatttaagc catggcttga tgttaaaagc aggccgtgat caggtgttgc tgcccgccat
      181 gcaggagatt gtgcacgcaa ttgccgccat ggcaaaagag tacgcagctg tgcccatgtt
      241 atcgcgcacc catggacaaa cggcttcccc caccactgtg ggtaaagaaa tggccaatgt
      301 ggcctatcgt ttagctcgac aaattacgca gataaaacag gtacagctgt tgggtaaaat
      361 taatggcgcg gttggcaatt acaacgcgca cttatctgcc tatcccgata ttaattggca
      421 ggcgcaagct caacacttta tcgaatccct cggcctaacc tttaacccct acaccacgca
      481 aattgaaccc cacgattata ttgctgagct ctttgatgtc atcgcccgct ttaacactat
      541 cttaattgat tttaaccgag atatttgggg ttatatttcg ctgggttatt tcaagcaaaa
      601 aaccattgca ggcgaagtgg gttcctccac tatgccacac aaagtaaacc ccatcgattt
      661 tgaaaattca gagggcaatt taggccttgc caatgcggtc atgttgcata tggcggcaaa
      721 actgcctatt tcccgctggc agcgggatct cacggattca accgttttgc gcaacatggg
      781 tgtaggtttt ggctattgcg aaatcgctta cgcatccgcg ctcaagggca ttcgcaagct
      841 ggaaattaat acccctgtta ttgcagccga tttagattcg gcctgggagg ttatggcaga
      901 gcctattcaa acggttatgc gccgcttcgc tattgaagag ccctatgaaa aactcaaagc
      961 acttacccgc gggcaaaaaa taacccaagc gattatgcag tcgtttgttg aacaattaga
     1021 tattccgccc accgccaaag cgcaattaaa agcacttacg ccaagcactt atgtgggcaa
     1081 tgctgagcaa caagctctgg atttattagc tcaactgcca caataaactt tcagcactgg
     1141 gattttctaa tgcagctgca cttggtgagc aacactccct agtgcagcgg gcagagttat
     1201 tggtagaatt accgccctcc agcgctgttt atgttcccgt agtttaatgg ataaaacgag
     1261 gccctcctaa ggcttagata caggttcgat tcctgtcggg aacgccattt cgccatcaac
     1321 accgccctgc cctacttagc cgaatacgcg gccattccta tgaatatctt gctattaaac
     1381 ccggatgatt taaaccccac cggtaccgtc actctgcgcg gcccaaaact tgagcattta
     1441 aataatgtgc taaaagttat accggggaaa ctcattaatg tcggccttaa aaattcttgg
     1501 ttggatcaag ccgtagtgga ggctataaat tccgaatttg tccagttgcg tttattgggc
     1561 aatcaacaac caccaccacc cgcgctccct tgcagtttaa ttttaggcct gccgcgcccg
     1621 cgcatgttac agcgcagcct gcaaactatc gccaccatgg gcgtggaaaa attattgttg
     1681 atcgccagtg agcgggtgga aaaaagtttt tggcaaacgc ccctattaaa accagcggcg
     1741 atccaagaac aattagaaat cggccttgaa caagccaaag ccacccaagt acccggggta
     1801 attttatgcc ccaattggcg ggaatttgcc cacactttat tgccagcact cagcagccaa
     1861 tgcctcaata aaattatcgc ccacccaggt gatgcgccgc gcgcaacagg tttagcggat
     1921 ggccagtctt tagtggctgt tgggccagaa ggggggtttg tggacagtga agtagcgctt
     1981 tttaaagagt tgggttttag ccaagtgcaa ttgggtgcgc gcatattgcg ggtggaaaca
     2041 gccatacccg tactcttggc gaaactattt taaatgggtt cattttttta aattggacag
     2101 acaggaaaac ggcataaaaa caacaggcga aaaaaaaagc ggcccaaagg ccgccttttt
     2161 aaacacgaca attagttgtt ttgttggctt tcgatctgag ctttgatcaa atcaccgatg
     2221 gtagccgggg ccacttgctc ggattgttta agatctttaa tagagctctt ctcgtcatca
     2281 acatctttcg cttttacaga aagaataata gtgcggtttt tacgatcaac gttggtgatc
     2341 ttagcttcaa ctacatcacc ttctttgagc acattgcgcg cgtcttcaac tttttcacgg
     2401 cttaggtcag aggcttttaa agtgccttca acatcatcag ccagaacaat tactgcgccc
     2461 ttagcgtcta cttctttaac agtaccctta acgatgctgc ctttgtcgta ggtgcctacg
     2521 taaacagtaa atggatcttg ctcaagctgc ttaataccta aggagatacg ctcgcgctct
     2581 gggtcaatgg ccaatacaac ggtttccaac tcgtcgcctt tcttgaattt acgcacggct
     2641 tcttcgccag tttcattcca agaaatatca gacagatgca ccaaaccgtc gatgccgcca
     2701 tcaagaccga taaagatacc gaagtcagtg atggatttga ttttgccgga gattttatcg
     2761 cctttagcaa actgcttgga gaacgcatcc caggggtttt cttggcattg tttgatacca
     2821 agggaaatac ggcgacgctc ttcgtcgatg tccaaaatca tcacttcaac ttcgtcgcct
     2881 aactgcacaa ctttgctggg gtgaatgttt ttgttggtcc aatccatttc agaaacgtgc
     2941 accagacctt caacaccggt ttcgatttca gcgaaacagc cgtagtcagt aaggttggta
     3001 atgcgcgctt taaccttggc accttctggg taacgctggg tgatagcagc ccatggatcg
     3061 tcgcccaatt gtttaagacc taaagataca cggttgcgct cacggtcaaa cttaagaact
     3121 ttaacttcga tttcatcacc aacagtaacg atttcgcttg ggtgtttaat acgcttccaa
     3181 gccatgtcgg taatgtgcaa caggccgtct acgccgccca ggtcaacgaa ggcaccgtaa
     3241 tcggtgaggt ttttaaccac acccttaacc gccatacctt cttgcagggt agccagcaac
     3301 tcatcacgct cgtcggagtt ggcttgctcc ataaccgcgc ggcgagagac cactacgtta
     3361 ttgcgttttt ggtcgagctt aataacttta aattcaagct ctttgccctc tagatgggta
     3421 gtttcgcgca ctgggcgcac atccactaaa gagccaggta agaaggcgcg aataccggca
     3481 acatccacag tgaagccgcc tttaacctta ccattgataa tacctttaac cacttcttca
     3541 gcggcgtggg cggattcaag tactttccaa gactctgcgc gcttggcttt ttcacgagaa
     3601 agacgagttt caccaaaacc gtcttcgacg gtttccaaag ccacttggac ttcgtcaccg
     3661 atgctcaggg ttaacttgcc gttctcatcg tagaattgct ccgcaggaat aacgccttcg
     3721 gatttaaggc ctgcgtgtac agttacccaa tcgctgtcga tatcaataac aacaccagta
     3781 actatgctgc ctggaaccat atcgacggtt tttaggctct cttcaaataa ctctgcaaaa
     3841 ctctcgctca ttacattacc tatgtttatt tagcttatgg cggatgcaaa catctgcctg
     3901 ccgctttacc agtcagcggg ccggttaatg aaacaagagc gccgccggta actggttggc
     3961 gcacgctcct aagcccattt gcacaggcaa aaaaggcgcg gggaaactac caaactatag
     4021 gagggatatc aacgactatt tgccaatgac cgcaaatact ttgctttaag aacactgatg
     4081 aagtgctgcg aaaagaatat ctagcgcgcg gggtgatcgt tagcctgctc aaggcgctta
     4141 agcaacttac gcaccttgcg ctgcagtagc cacaatctgg gcaacaacag gataatcccc
     4201 aacaatgcgc caacccccaa ggacaccata aaaatgaagc cggcgctggc ctcgtagact
     4261 ttgccgaata aatgcagggt gattaattgc gggttgtcag cggtaatagc ggcactcaaa
     4321 aacaccaaca ccaacaacca gagcaataca aacaggcgtt taagccaaac caggaagcgc
     4381 agcattaggc ttgcacctcc cgccaatact gcagaatcgc atccaatacc tcctggatgg
     4441 acagggcggt gctatcgagc aacttggcgt catcagcagg ccgcaggggc gcaaccgcac
     4501 gctgggcatc ctgggcatca cgcttttcta tatcagcggt tatttgctca aggtcagctg
     4561 tgaggccctg ctgctgtaat tggtcgtagc ggcgctttgc tcgcaccgca gggctggcgg
     4621 ttaaaaatat ttttaatggt gcatcaggga ataccaccgt ccccatatcc cggccatcgg
     4681 caataaggcc cggcaactgg cgaaaatccc tctggcgctg caataaagcc tcccgtacgg
     4741 caggtaaagc cgcaaccttt gaggcgccca tgcccacatc ttcagctcgg atagcatcag
     4801 taacctctcg gccagccaag aggataacga cctgcccatt gcgcagggag aattcaatat
     4861 ccagggtgcg cgccaaagcg gcgaggccgt gttggtctgc cagatccact ccctgcccca
     4921 tggccgctag cgcagtcaaa cggtataggg cgccgctatc taaaatggta aaccccaaac
     4981 gctccgccac taaacggctc actgtaccct tgcctgcgcc actggggccg tcaattgtaa
     5041 ttacagggat cataaacaag ccttaataag tggacttttc gtgaattaat ctgctgacca
     5101 aatttgcacg cggaaaaatg gaaaaaacac tcaagtgctt cctgccgtat aggttgcagg
     5161 ccctatgccc gcgacgcatt caaaataccc acgcctgcaa atatcagcag gcgcttacgc
     5221 atccagatta agggattggt aaagctcata acaggcctgt gccgggtcgg ccgcttgggt
     5281 gatgggccgc ccaaccacca agtaatcact gcccgcctca agggcggcgc tgggtgtaag
     5341 cacccgggac tgatcctggt tatcagcgcc caatggccga atgccgggag tcaccgccaa
     5401 caacgcagca cccgcctgtt gtttaatcgc acgggcttcc aaggcagaac acaccacccc
     5461 atcaagcccc gcctcagcag caaggcgtgc taaaaaactc acccgatcgg cgatggcccc
     5521 tgcaaagcct gtctgcgcaa atacctcttg agacatgctg gttaacactg tcacggcgat
     5581 gagaagcggc ggtgaagaga attttcccaa ggcagctttg gcctcctgca tcatgggcaa
     5641 gccgccgctg gcgtgtacat tcaccatcca aacacccagt tgcgccgccg cagccaccgc
     5701 cttggcgaca gttgccggta tatcgtgaaa tttaagatcc aaaaaacatt caaaaccctt
     5761 ggcctgtagg gtttccactg cctgggggcc agcggcggta aacagttcct tacccacctt
     5821 cacacgacaa tatcgggggt ctaaccgttc ggccaaagcg taggccgctt ttgcatcggc
     5881 gtaatccagc gcaacaatta ggggcgaggc cactgacatg ggtacaactc caggggtaaa
     5941 aataaaaaag cccaacccag gtatatgagt tgggctttaa tgtggactac ttctttaaat
     6001 tacattttta cttttgcggc tgaaaaatgt gcgccttcat aggcggctat atcatcggtg
     6061 cttttttgtt gcacaacctg ggatttaata ccggccaatt gctgagcctc aagatcggca
     6121 accgtaccgt tggtggcagc cgttaggcta atcaccactt gaccgccgct acgcgtagca
     6181 aaattttcca cctgtggctg ggcagctggg cgcaccatac caaaggcgta ttgcagaatc
     6241 tctcgatcag ccgtggattt aaagcgatcc tgcttctcaa attgtgcgaa ggtgtagccg
     6301 agttctttag cggcatcttc aggttttgca cccgcacgca gcttatcgct cagggcctta
     6361 gccagctgct gtaattgcgc tgcggttttc tgttgcacta atgtctccac aatcgcaccg
     6421 cgcacttcgg ccaaagcttt cacatggctg ggtttatgct cttttaaacg cactaccaca
     6481 gcgcgctcct ggggcaattc taaaacctta ctaatttggc cctgctcaag cacatccttg
     6541 gcaaaggcgg cttctaacac tagtggctct gaggtaatac cggcagcacc tgttttggca
     6601 aacagctcgg tctcttggat atttaggcct agcgcggtag cagcgctggc taaatcgctt
     6661 ttaccaaagg tagcttcttc taaggattta atttgatcga caaatttctc ggcggcttta
     6721 tcggtagcaa gctgctgggc gagggctgag cggcgacctt caagggttgg cggcacaagc
     6781 tgggttttct tcaccaattt aacaaaatga gtcccagcgc tggatttaag tggcccagaa
     6841 acctggccct cttttaatgc cgcaacagcc gttttaaatt cggcaggaaa actatcgccc
     6901 gtcataacac ccaggtcacc accgagttct ttggagccca ggtcgtcgga ataggttttg
     6961 gccaaggcgt caaaggcctc ccccgcattg atcttggctt gtacctgagc gattttggca
     7021 tcactctcgg cgctgttttt acctgcctca atcaggatat gggcaatact caactcaggc
     7081 tcagatttaa attcagccag ttctttatca aaagccgctt tgatttcttc ctcagtcacc
     7141 gcctggctct tcgccaactg agccacagat acctcaaggt attgcaagga gaccttctct
     7201 ggctcactaa aactgctttg attatcctgg tagtactgag caatctcctc ttcagtaggg
     7261 gtgacttttg cggccttctc tttaggaatg gtaacggcgc taaaggagcg cttctggccg
     7321 acaatacccg ctaacaactc aacatcctgc tgggtgataa aggctgtgtc gctgagcccg
     7381 ctgagcagct ggcgcactat gtaggtttcc gattcttgct gcagcaaagt cgcttgggtg
     7441 aaaccgaaat tggccaggta ggcgttaaaa ccctgggcgt taaattgttt atcttgtgta
     7501 aaggcctgct tgatttgatc tttaacgacc acttccgaca cgcccattcc cgcacgctgg
     7561 gccgcttgga tcacagcgcg ctggcgcata aggctggtga gcgccggttg cttgaggttt
     7621 tcgtctttga gctgctctgc gtcatcaggc ataccaaact ggttctttag gcggattttc
     7681 tcctgggtca tggcgcggcg cagatccttc agtgagatat catcaccatt cacactcgcc
     7741 acagtgtcgg taaacgaggt tttagaaacc tcttgtcccg ttactatcat ggctaacaga
     7801 actaccgcac caattacgac tgccacggtg ccttttaaat tttcacgaag gtcttggatc
     7861 atagagggaa attccaacag agcattttcg atttaccgac aataaaaagg cgcatcaatg
     7921 cgcccttttg acatccacca ccgccttgcg gcggcaaatg aattaattga ctgcgtcttt
     7981 caacgcctta cctggtttaa aggaagggat tttggcagcc gctatgtcga taggtgctcc
     8041 ggtttgagga ttgcggcccg tacgtgcagc acgctctttt acagcaaagg ttccaaagcc
     8101 cactaaagca acttgatcac ccttttttaa tgcgcccatg atggactcag ttaccgcgtc
     8161 caatgcacgg ctagcagcgg ccttagaaat atctgcagaa gcagcaatgg catcgattaa
     8221 ttcggactta ttcacgtaac aacccctctt attattaggt acgtaatggt taaaaattct
     8281 tcagtcttac aactcaacaa tgaatatgta caacaacccc gagccttagt tagatccaag
     8341 gaggttgcga tttataccaa caggcgccag accggtcaag aaaactctca aggaaatccg
     8401 aaacggcgcc tcatttagcc ggttagtggg tacttatgcc gggggaatct ttgggtaaat
     8461 cgaacgcctt gctctctaag gctgcgaatt catcatcact aaacccagcg ggtgcatggg
     8521 ttaaggctac cgctaatacc tcttcaatgt gacgcacagg tttgatgatg agatccgcct
     8581 tgatgttatc gggaatttcc cgtaaatcgg cttcgttgtc gcctggaatc aacaccgttc
     8641 taatcccccc gcggtgggcc gccaataatt tttctttcaa cccaccgatc ttcaatacct
     8701 gcccgcgcag ggtgatctcc cccgtcatgg ctacatcggc acgcaccgga atacgggtta
     8761 acgccgacac tagggcggtg cacatagcga tacctgccga tggaccatcc ttaggggtag
     8821 cgccttcggg gacgtgaata tggatgtcat gtttttccgt cgcatcaggg gcaataccca
     8881 gtactttcgc ccgcgagcga acaaccgtaa atgccgcctg tatggactcc tgcataacat
     8941 cgcccaggga gccggttttt accaatcggc ctttgccgcg caccgaggcg atctcgatgg
     9001 ttagcagctc tccacccacc tgggtccagg caaggcctgt aacctggccg atgcggtctt
     9061 cagtatcggc gcggccataa tcggtctttt taacgcccag gtaatgctcc aggtgttctg
     9121 acgtgagtac tctaggcgcg ctctttttag acttaacatt ttcggtcact accttgcggc
     9181 acagcttggc aatttctcga tccaaactgc gcacaccggc ctcacgggta taaaagcgga
     9241 taatgtcccg cagggcgctc tcttccacct ttaattcatc atcgcgcagt ccacagtttt
     9301 tgcgttgttt gggcaacaaa tagcgcatgg cgatgttgag cttttcatcc tctgtatagc
     9361 cgggaatacg gatcacctcc atgcgatcca gcaacggccc ggggatgttc atggtgttgg
     9421 aagtacacac aaacatcaca tcggagaggt cgtaatccac ctctaaatag tggtcattaa
     9481 atttattgtt ttgctctgga tctaatactt ccaaaagggc cgaggcgggg tcaccgcgat
     9541 gatccatacc catcttgtcg atttcatcga acaaaaacag cgggtttttc acgccggttt
     9601 tggcaatttt ctgcaacaac ttgcccggta aggagcctat gtaggtgcgg cggtggccgc
     9661 gaatttccgc ttcatcgcgc acgccaccca gggccatgcg cacaaacttg cgattggtgg
     9721 cgcgggcgat ggactcaccg agggaggttt taccaacccc cggtggcccc actaaacaaa
     9781 tcactgggcc tttgattttt ttcacgcgtt tttgcacggc caaatattcc aagatgcgct
     9841 ctttcacctc agtcaatccg tagtgatctt cttccaacac cttttccgcg tgggccagat
     9901 ccatgttgat tttgctgcgc ttgttccagg gcacattcag catccaatcc acataggagc
     9961 gaataaccga agcctccgcc gacatggggg acatcatctt taatttatta atttccgcgc
    10021 gggttttatc ctccgccgct ttgggcattt tcgcatcgag aatgcgccgc tcgaattcat
    10081 cgccctcccc ggcgtcttca cccagctcac ccagctcttt ctgaatggcc ttgagctgtt
    10141 cgttgaggta atactcgcgc tggctttttt ccatctgttt tttaacgcgg ccgcgaattt
    10201 ttttctccac ttggtaaagg tccgcctcag catccatcaa accgatcaaa tgctccaggc
    10261 gctcaccgac tttaaatagc tccagcacac gctgtttctg ccccaaatcc aaggtcatgt
    10321 gcgcagcgat ggtatccacc aggcggccgg gttcatcgat accattgagg gagttgagaa
    10381 cctctacggg cacttttttg ctgaagttta catagtgctc aaagcgcttt aacaaccctc
    10441 gcgccatggt ctcctcttca cgggtgtaga ccttatcggc gggaatagtc gtgcaatggg
    10501 ctttaaagca gcgctcggat tcatccaccg attctatctg cgcacggc
//
LOCUS       DAOQGS010000071        10485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2960_length_10485_cov_4.759252, whole genome shotgun sequence.
ACCESSION   DAOQGS010000071 DAOQGS010000000
VERSION     DAOQGS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_10485_cov_4.759252"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PKZ52_04150"
     CDS             complement(<1..1642)
                     /locus_tag="PKZ52_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNG58994.1"
                     /translation="MQQRIRIGDILVAQQIISQDQLRLALEGQKNTGNKLGKQLIVMG
                     FIEENTLLSFLSKQLNYPFVELKQFRFDRDLIQTLPETYARRYRVLVLKRDSDGILLG
                     MADPTDIFCLDELQKVLRAPIRPAVVRESELMDILDTAYSRASEIASLAEQLDEELGT
                     DAADLTGLLQSAEDLDAPVVKLLQKVFEEALSTRASDIHIEPDESVLRIRQRIDGVLI
                     EQVMNEKRISAALVVRLKLMASLDISEKRLPQDGRFNLKIGHRNIDVRMSTMPVQFGE
                     AVVMRLLDHSAGVRKLDAVGMPPAYVARFRQIITRPHGLVLVTGPTGSGKTTTLYGAI
                     SELNTAERKIITVEDPVEYRLSRISQVQVHDKIGLNFAKVLRACLRQDPDVLLVGEIR
                     DSESAEIALRAAMTGHMVLSTLHTNDAVGSALRLIDMGVDAYLVASSLKAIVAQRLVR
                     VICDVCSQPHTPDEQDLLVLRQYQRYLGQKPLNLRKGQGCPHCYNTGYHGRAGVFELL
                     EINSVMANALRDNSVRQFNDAAHQHPNYRPLAASALDLA"
     gene            complement(1988..3394)
                     /locus_tag="PKZ52_04155"
     CDS             complement(1988..3394)
                     /locus_tag="PKZ52_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58995.1"
                     /translation="MSLINDVLEQIDARAPVAEPERVVIEPTAYTDSATKFFTLSPHL
                     KIGALALCGFLSGYGSLALFNVLASPAQIKNTTQNDTGLIFPAARQLHSNLDANTANA
                     NAPNEMTLTGQLNPINPVAPQTAQAEPDEETTMLGQAKQYFLADRLTYPPLHNAYQLY
                     REILLKNPNNLAALEGIASIKQRYLALTTEAINQGVWEKAQRYIERAEFVGVDRQELN
                     RLRAQLQGLMPAETAASGDTRTAEPVNIGSEQNSVLASENRPAAPVGEQEQLTLNPPS
                     GNRLHRNNTGGALPVSEQAFLASLVEDKDSERLALDFIKSHGNSADTVRWLARRWVSA
                     QQWLPLLDLMSSPSAINPAERDVFRAQALLGLKNYQELIVWLSATQEGEQPELQRILA
                     VALQKTGRETDAMAIYQHLVARHPDNSGLWLALGLSADGLGNNTVAREAFFRAQQLGG
                     HSKTVSDFLAKKLAAAPR"
     gene            complement(3432..4346)
                     /locus_tag="PKZ52_04160"
     CDS             complement(3432..4346)
                     /locus_tag="PKZ52_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG58996.1"
                     /translation="MYLKFFGFKELPFSLTPDTSYFYAYHSHQNALNTLLVALHNSEG
                     FIKIVGEVGTGKTLLGRLLLKKLHTSRFVTAYIPNPHLSPDELKLTLAQEIGVPDCTS
                     VPHFQLLGHISRRLIELAVQKKRVVLVLDEAQTMPRETIESLRLLTNLETEKRKLLQV
                     VMLGQPELDDLLARKDLRQLKQRIVFSETLKPLGFSALCSYIRFRLNAANPTAAHLFN
                     RRALVVLFFASGGVPRLINLLAHKSLWSAFGRNQLRVTWQDVRTAVQDTPEARPVARW
                     LLGGNAAKYWLTYGLAAASGALVQWLGY"
     gene            complement(4349..6091)
                     /gene="mshL"
                     /locus_tag="PKZ52_04165"
     CDS             complement(4349..6091)
                     /gene="mshL"
                     /locus_tag="PKZ52_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HNG58997.1"
                     /translation="MHWLQASIKSDTAALGNLKCWRAAWLGMVLCGLLSGCANTKNAS
                     TQLDAEIDKLTEQDKAPVPQEVQNAVLDNRLDARSGPRRFDVAVNNMAARTFFNALAA
                     DASVNIVTDPDVTGAVSLQLRQVTLDEVLDVTRDMFGYEYHKQGGIYTVYARQMRTQV
                     FQINNIDVTRRGQTDTQVNIGSTASNSSSNASTPSSGGESAAKETTSSENNSGARVQT
                     KHESHFWADLQNSLEAIVGSEDGRSVIVNPAAGLVVVKALPKDVNAVRNFLEKSELSI
                     KRQVILETKILEVRLNNGFEAGVNWNAISGQLVYGYNVQPGFGINGDGATVDRWRNWG
                     DVNRSYTDAQGVDTTIGSTYAEKTGGVFASMLQVNDISKLLSLLKTQGDVQVLSSPRV
                     STVNNQKAVIRVGSDEYFVTGITNNTTSSAAATTNSPNIELASFFSGISLDVTPQIAD
                     NGDVILHVHPMVSDVQDQQKNLTVGDQKFSLPLALREVRESDSVVRARSGQVVVLGGL
                     MQETSKKLNGKRPLLGDIPVLNTLFKTKSSSGNKTELVILMRPVVVSENTWQDQLKEE
                     SERFKVLTEELRKR"
     gene            complement(6134..6496)
                     /locus_tag="PKZ52_04170"
     CDS             complement(6134..6496)
                     /locus_tag="PKZ52_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58998.1"
                     /translation="MNRRIYGCILLAACWYVQAEEPPLKDPTTPFNSVRNAPVNNERA
                     PVKNAGLRWVLDGVLISDDGKKALVNGRWYNERDQINGWQLVQVETAAVVLKQAGKTQ
                     RILLYPSLTKNTLTLKPE"
     gene            complement(6489..7082)
                     /locus_tag="PKZ52_04175"
     CDS             complement(6489..7082)
                     /locus_tag="PKZ52_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG58999.1"
                     /translation="MVFELAILGPAQRKHSQLAADAETQKKQFESLKAEEVVLTKVLT
                     TDPALKLKKEYEQLSLQLESLDKEIIDLSAGLVSAGQLAAMLQEVLVLTDGLALEGLT
                     TEPPLSIPLPSDGVKSQDKQAKPTTTPAAQLRIYKQYIALKLKGNYFQILNYLKRLEA
                     TRWHFYWEALFYQVSQHPEATVEIRVYSLSIERPDHE"
     gene            complement(7183..7830)
                     /locus_tag="PKZ52_04180"
     CDS             complement(7183..7830)
                     /locus_tag="PKZ52_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59000.1"
                     /translation="MQQVNLYLKELRPKFDPFAARPLLMFTGFFVCCLLLWAGFAHTR
                     VNNMQAVVDTVVADVRNEEAKLSRLKVLGKPGDKTQLEDSASQVRTAIDNREFIKRLI
                     SSNTFGNDRGFSVLLTALSKAALKDLFLREFGVAAGGRDVYLHGITTRVDAVPEYIKI
                     LQQDAAMAKVNFGVLKLLKAAGGQIEFSSGLWDEQVMGAKPGDDPQAAAATGQGR"
     gene            complement(7832..8758)
                     /locus_tag="PKZ52_04185"
     CDS             complement(7832..8758)
                     /locus_tag="PKZ52_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59001.1"
                     /translation="MLLRVNQAQPQSLVGLELQADGIAVAVKVVKSQIPHIARLAFLP
                     GEDEKQRFAQLRDYVKQNKLKNKPCNLVLQQSRYQLYLLEAPDVPAAELANAMKWRLK
                     DLVNQPLDQLAIDVFHVPNDGARGNRKMIYVVVVNKSYLQELIEFVQQSELKLLAIDI
                     AELALRNLAIHLVDPQFDQRGVAIARVRQGAGSVSIYRAGDMYLSRQFDLAYGGGLLD
                     DLPAESLALELQRSVDYYERQMGQLSPAVVYLGGENIADEKITASLRKILSVPLQFLD
                     FSGAVTMESEFESGIMQLGLGALGAAMRQEFA"
     gene            8901..9365
                     /gene="trmL"
                     /locus_tag="PKZ52_04190"
     CDS             8901..9365
                     /gene="trmL"
                     /locus_tag="PKZ52_04190"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNG59002.1"
                     /translation="MIHIVLFEPEIPPNTGNIIRLCANTGCQLHLIEPFGFALDDKKL
                     RRAGLDYAEWKDIKIYKDWEVFLRELAPPRWFAITTKGSQNYTKVAFAAGDALVFGPE
                     SRGLPPAILDSAAPERRLRIPMRPGSRSMNLSNAASVMVYEAWRQLDFNGAL"
     gene            complement(9398..9706)
                     /locus_tag="PKZ52_04195"
     CDS             complement(9398..9706)
                     /locus_tag="PKZ52_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNG59003.1"
                     /translation="MTEHAIQQKLHAAFSPSVLQVENESHHHSSRPGNNTHFKVTLVS
                     DAFSGLTSVARHRAVYAVLKEELSGGVHALALHTYAPDEWQGDSPASPRCMGGEKKPN
                     "
     gene            9830..10045
                     /locus_tag="PKZ52_04200"
     CDS             9830..10045
                     /locus_tag="PKZ52_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59004.1"
                     /translation="MSSSIIFVNRRKGRDRRQDADPCKEMPLDLFHRKRRKSKERRDT
                     SRTLTDDYYASMQKTLASLQNEQTGKP"
     gene            10123..>10485
                     /locus_tag="PKZ52_04205"
     CDS             10123..>10485
                     /locus_tag="PKZ52_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59005.1"
                     /translation="MSLLDPKELKLVVTLLQRQRMAMAKTQATLHDEKQSAELAATLK
                     LLDGALNKLVASGTKSVANPTPSPLPAKKPIKRREPIPLADALVLVADDSIDSMMMLR
                     GILSDLGIKKIDSVKDGRE"
BASE COUNT         2715 a         2845 c         2269 g         2656 t
ORIGIN      
        1 cagctaaatc cagggcggaa gccgccagcg ggcgataatt agggtgctgg tgggcggcat
       61 cgttaaattg ccgcactgaa ttatcccgca gcgcattcgc catgacgcta ttaatttcca
      121 ataattcaaa cacccccgct cggccgtggt accccgtgtt ataacagtgc gggcagccct
      181 gccccttgcg cagattcaaa ggcttttggc ccaaataacg ctggtattgt cgcaatacca
      241 ataaatcctg ttcatccggc gtgtggggct ggctgcacac atcacaaata acccgcacca
      301 agcgctgcgc cacaatggct tttaaactcg acgccaccaa ataagcatct acccccatat
      361 caattaggcg cagcgccgaa ccgaccgcat cattagtatg cagagttgat aacaccatat
      421 ggcccgtcat agcggcgcgc agtgcaattt ccgcagattc agaatcccga atctccccca
      481 ccagcaacac atctggatct tgtcgcaaac acgcgcgcaa tactttcgca aaattaagcc
      541 caattttgtc gtgcacctgc acctggctta tgcggcttaa tctgtattcc actgggtctt
      601 caacggtaat tattttgcgc tcggcagtat ttaattccga aatggcgccg tacaacgtcg
      661 tagttttacc gctgccagta ggcccagtta ctaatactaa gccgtggggc ctagtaataa
      721 tttgccgaaa ccgcgccaca taagcaggcg gcattcccac tgcatccaat ttacgcacgc
      781 ctgcactgtg atccaataac cgcatcacca cggcctcgcc aaactgcacc ggcatggtgg
      841 acatacgtac atcgatattg cggtggccaa tttttaaatt aaaacggccg tcttggggca
      901 ggcgtttttc agaaatatcc aagcttgcca tcagctttaa gcgcaccact agtgcagcgc
      961 taatgcgttt ttcattcatc acctgctcaa ttaatacgcc atcgatacgc tggcgtatgc
     1021 gcaatactga ttcatccggc tcaatatgaa tatccgacgc ccgcgtgctc aatgcctctt
     1081 caaatacttt ttgcaataat ttaacaactg gcgcgtctaa atcttccgcc gattgcaaca
     1141 aaccggttaa atccgccgca tccgtgccca gctcttcatc taattgctcg gctagggaag
     1201 caatttcgct ggcgcggctg taggcggtat cgagaatatc catcaattcc gattcccgca
     1261 ctacggcagg acggatcggc gctcgcaata ccttctgcaa ttcatctaga caaaaaatat
     1321 cagtagggtc cgccattccc aaaagaatgc catccgaatc ccgtttaagc actaataccc
     1381 ggtaacgcct tgcgtaggtc tccggcaggg tttgtattaa atcgcgatca aaccgaaatt
     1441 gttttaattc tacaaacggg taatttaact gcttggacaa aaacgaaagt agggtattct
     1501 cttcaataaa ccccatcaca ataagctgct tgcccaactt attgccggta tttttttgcc
     1561 cttccaaggc taagcgcagc tgatcctgac tgataatctg ctgcgcaact aaaatatcac
     1621 ctatgcgtat acgctgctgc atagcatctt taaccccatt gttaccaccc aattaaacgc
     1681 ctagcagcgt gttggaatac tcaaaatcca agcctgacgg cgatcctctt actagctgta
     1741 cggaagcaat gaatacacat catttcagac cgtcaaaaag agggaccttt ttgacgagcc
     1801 tacacggatg tatttacggc gtgtcagaaa tgatgtgtat tcattgcggg tgcctagtta
     1861 gaaagcgaag cgcccagcac tcaaccactc agcgcaaacc gcaacaatta ctactcaatt
     1921 gccccacttc aatttcattc cccataaaca agcgcaatgg ctcgccacat ccacagccaa
     1981 tatcctacta acgcggtgca gcggctaatt ttttcgccaa aaaatcgctg accgttttac
     2041 tgtggccacc caattgctgg gcacggaaaa atgcctcccg cgctacagta ttgttaccta
     2101 atccatcggc acttaatccc agcgctaacc aaagcccgga attatccggg tggcgtgcaa
     2161 ctaaatgttg gtaaatcgcc atagcatccg tttctcggcc agttttttgt aaggccacag
     2221 cgagaattcg ctgcaattct ggctgctcac cctcttgggt tgcgctgagc caaacaatta
     2281 attcttgata attcttcagc cccaatagcg cctgggcgcg aaacacatcg cgctcggcag
     2341 gattaatagc actgggcgaa ctcataagat ccaataaggg cagccactgc tgcgcactga
     2401 cccaacgccg cgctaaccaa cgcaccgtat cggcagaatt gccgtggctt ttaataaaat
     2461 ctaacgccaa acgctcactg tctttatcct caaccaggct tgccaaaaac gcctgctcac
     2521 tcacgggcag agcaccgcct gtgttatttc tatgcaaccg attgccagag ggcgggttta
     2581 aggtaagttg ttcctgctcg cccacaggcg ctgcaggcct attctcactg gctaaaacac
     2641 tattttgctc tgagcctata tttaccggct ccgctgtgcg cgtatcaccc gaagcagccg
     2701 tttccgctgg catcaaccct tgcaactgtg cgcgcaaacg atttaactcc tgcctgtcaa
     2761 cgcctacaaa ttccgcccgc tcaatatagc gctgcgcctt ttcccatacc ccttgattaa
     2821 tcgcttccgt tgttaaagcc aaataacgct gtttaatact ggcaattccc tctaaagcgg
     2881 caagattatt aggatttttt aataaaattt cccgatacag ttgataggcg ttatgcaatg
     2941 gtgggtaggt taagcggtct gccaaaaaat attgtttggc ttgtccaagc attgtagttt
     3001 cttcatcggg ctcagcttgg gcagtttgcg gcgccacagg gttaatgggg tttaactgcc
     3061 cagttaacgt catctcattt ggcgcattcg cattggccgt attagcgtca agatttgaat
     3121 gcagctgacg cgctgcagga aaaataagcc cagtatcatt ttgtgttgtg tttttaattt
     3181 gcgcagggga tgctaaaaca ttaaaaagtg ccaaggagcc ataaccggat aaaaaaccac
     3241 acagagccaa cgcacctatt tttaggtgtg gtgagagtgt aaaaaatttt gttgctgaat
     3301 ccgtataagc ggtgggttca attacaacac gctcaggctc agccacaggc gcgcgagcat
     3361 caatttgctc taatacgtcg ttaattaagc tcacgtttat cctcctgcgc cggttttaag
     3421 cgggcctaca tttaatagcc cagccactgc actaaggcgc cgctggcagc ggctaatcca
     3481 taggtcagcc aatatttcgc tgcattgccg ccgagtagcc aacgggccac gggccgagct
     3541 tcgggcgtgt cctgtacggc ggtgcgcaca tcctgccacg ttactcgcag ttgattgcgg
     3601 ccaaaggccg accaaagtga tttgtgggcc aataaattaa ttagccgcgg cacaccacca
     3661 ctggcgaaaa acaacaccac taacgcgcgc cggttaaata aatgcgcggc ggtgggattg
     3721 gctgcattta aacgaaaccg aatataagaa cacagtgccg aaaaccccaa gggcttgagt
     3781 gtttctgaaa acacaattcg ctgctttaat tgccgcaaat ccttgcgtgc caataaatca
     3841 tccagctctg gctgccccaa cattaccact tgcaataatt tacgtttttc agtttctaaa
     3901 ttagtcaaca accgcaggga ttctatggtt tccctcggca tggtctgagc ttcatctaaa
     3961 accagcacaa cacgtttttt ttgtactgcc agctcgatta aacggcggct gatatggcct
     4021 aataattgaa aatgcggcac cgaagtacaa tcgggcacac cgatctcctg ggccagggtt
     4081 aatttaagtt catcagggct taggtgtgga ttgggaatat aagcggtaac aaagcgagaa
     4141 gtatgaagct tttttaataa caggcgcccc aaaagggttt ttccagtacc cacctcgccg
     4201 acaattttta taaaaccttc gctgttatgg agggcaacca gcaaggtatt taaagcgttt
     4261 tgatggctgt ggtaggcgta aaaataactg gtatccggcg ttagcgaaaa gggcaattct
     4321 ttaaagccaa aaaattttag gtacatacct acctcttgcg caattcttca gttaatacct
     4381 taaatcgctc cgactcctct tttaactgat cctgccaggt attttcactg accactaccg
     4441 ggcgcatcaa aatcactaac tctgttttgt taccggatga actcttggtt ttgaacaggg
     4501 tgttcaacac tggaatatca cccaacaaag gccgcttgcc attgagtttt ttactcgtct
     4561 cttgcattaa cccgcccaac accacaacct ggcctgagcg tgcgcgcacc actgaatcac
     4621 tttcccgcac ttcgcgcagc gctaacggca gtgaaaattt ttgatcgcct acggtaagat
     4681 ttttttgttg atcctgcaca tcgctcacca tagggtgaac atgcaaaatc acatcgccgt
     4741 tgtcggcaat ttgcggcgtt acatccaaag aaataccgct aaaaaatgac gccagttcaa
     4801 tattgggtga attggtcgta gccgctgcac tggaagtagt attgttggta atgccggtga
     4861 caaaatactc atccgatccc acccgaataa ccgctttttg gttattcacc gtgctgacgc
     4921 gagggctgga taacacctgc acatccccct gggttttaag caaggataat aatttggaaa
     4981 tatcattcac ctgtagcata gaggcaaaaa cgccgcccgt tttctccgcg taagtactgc
     5041 cgatggtggt atcaacgccc tgtgcatcgg tgtaactgcg gttaacatcc ccccaattac
     5101 gccagcggtc caccgttgca ccatcaccat taatgccaaa acccggttgc acattgtagc
     5161 catacaccag ctggccgcta atggcattcc agttaacacc ggcctcaaag ccattattta
     5221 accgcacctc cagtattttg gtttccaaaa tcacttgccg tttaatactt aactcagatt
     5281 tttccaaaaa attgcgcaca gcgttgacgt ctttaggcaa ggctttcacc accaccaaac
     5341 ctgccgctgg attcacaata accgagcgcc catcctccga gccgacaata gcctctaggg
     5401 aattttgtaa atccgcccaa aagtggctct catgtttggt ttgcacccgc gcaccggaat
     5461 tattttcact gctagtggtt tcttttgcgg cggactcgcc gccactggat ggtgtagatg
     5521 cattgctgga tgaattactg gcggttgaac ctatattaac ctgagtatcg gtttgtccgc
     5581 ggcgagtgac atcgatatta ttaatttgaa atacttgcgt gcgcatttgc cgcgcatata
     5641 cggtatagat gccgccctgc ttatgatatt catagccgaa catatcgcgg gtaacatcta
     5701 atacctcatc caaagtcacc tggcgcaact gtaaactcac cgcgccggtg acatccgggt
     5761 cggttactat attcacactc gcatccgccg ccagcgcatt aaaaaaggtc cgagccgcca
     5821 tattatttac cgccacatca aaacgacggg gaccgctgcg tgcatccagg cgattatcca
     5881 gtaccgcatt ttgcacttct tgaggcacag gcgccttatc ctgttcggtt aatttatcta
     5941 tttcggcatc taactgcgtt gaggcgtttt tagtattggc gcatccgctg agcagcccac
     6001 acaacaccat acccagccag gccgcacgcc aacactttaa gttgcccagc gccgccgtat
     6061 cgctttttat acttgcctgc agccaatgca taacacaccg ctgcgtccat ttttcacacc
     6121 acatgagatt aaattactcc ggcttaagag ttagcgtatt tttagtcagg ctgggataaa
     6181 gtaaaatgcg ttgggttttg cctgcttgtt tcagcacaac agctgcagtt tccacttgca
     6241 cgagctgcca accatttatt tgatcgcgct cgttatacca gcgcccattt accagcgctt
     6301 tttttccatc gtcactaatc agcactccgt ctaacaccca gcgtaggcca gcatttttga
     6361 caggtgcacg ctcattattc acgggcgcat tgcgaacact attaaaaggt gtagtggggt
     6421 cttttaaagg cggttcttcc gcctgcacat accagcacgc cgctaataat atacagccat
     6481 aaatgcgtct attcatggtc aggtcgctca atagataagc tataaacacg aatttccaca
     6541 gtcgcctctg ggtgctggct cacctgatag aaaagtgcct cccaataaaa atgccatcgg
     6601 gttgcctcca aacgtttcag ataatttaag atttgaaaat aattaccctt caatttaaga
     6661 gcgatatatt gcttgtaaat acgcaactgc gccgcaggtg tagttgtagg tttagcctgt
     6721 ttatcttggc tctttacgcc atcactcggc aacggaatag acagcggcgg ctccgttgtt
     6781 aagccttcca atgccaggcc atcggttagc actagcacct cttgcaacat tgcggcaagc
     6841 tgccctgccg aaaccaaacc tgccgataaa tcgataattt ctttatccag ggattcaagc
     6901 tgcagactta attgttcata ttcttttttt aacttcagcg caggatcggt ggtgagcact
     6961 tttgttaaca ccacctcttc agctttaagt gattcaaatt gttttttttg tgtttcagca
     7021 tctgcagcca gctgggagtg cttacgctgg gcaggcccga gtatcgccaa ctcaaacacc
     7081 atgtaaatgg ccgcaaaaat agaaaacgcc accaatagac gctcgcgcac aagcaaacca
     7141 ttaaatattt tttcccagtg ttttaatcgc gcgtgcatat tactaacgtc cctggcctgt
     7201 ggctgccgcc gcctgaggat catcacccgg cttggccccc atcacctgct catcccataa
     7261 ccccgaacta aattcaattt gcccaccagc ggctttgagc aattttaaaa cgccgaaatt
     7321 taccttcgcc atagcagcat cttgctgcaa aattttaata tactcaggga ctgcatccac
     7381 tcgcgtggtg atgccgtgca aatacacatc tcgcccgccc gcagccacgc caaattcacg
     7441 taaaaacagg tcttttaatg ctgctttgga taaagccgtg agcagaacag aaaaaccgcg
     7501 gtcattgcca aaggtattac tactgattaa ccgcttgata aattcacggt tatcaatcgc
     7561 cgtgcgcact tggctggccg aatcttccag ctgggtttta tcccccggct tacccaatac
     7621 ttttaaacgg cttaatttgg cctcttcatt gcgcacatcg gccactacag tatccactac
     7681 cgcctgcata ttgttgacgc gcgtgtgagc aaaaccggcc cacagcaaca agcaacagac
     7741 aaaaaaacct gtaaacatca acagcgggcg cgcggcaaac gggtcaaact taggccttaa
     7801 ttcctttaaa taaagattga cctgttgcat gctaggcaaa ctcctggcgc atagccgcgc
     7861 ctaatgctcc taagccaagc tgcataatgc cactttcaaa ttcactctcc atagtgacgg
     7921 caccggagaa atctaaaaac tgcagcggca ctgataatat tttgcgcaag ctcgccgtaa
     7981 ttttttcatc cgctatattt tcacccccaa ggtacaccac agcagggctt aactgtccca
     8041 tctgtcgctc gtaataatcc accgaacgct gtaattccaa cgccaacgac tctgcaggta
     8101 aatcatccaa caggccgccg ccgtaggcta aatcaaattg ccgagataaa tacatatcac
     8161 cggcgcggta aatggacacc gagcctgcac cttggcgcac acgcgctata gccacaccgc
     8221 gctggtcaaa ttgtggatcc accaaatgaa tggccaaatt acgcagagcc aattctgcaa
     8281 tatcaatggc caataattta agctcgctct gctgaacaaa ctcaattaat tcctgcagat
     8341 aacttttatt gaccaccaca acataaatca ttttgcggtt gccgcgagcg ccatcgttag
     8401 gcacgtgaaa aacatctatc gccaattgat ccaacggctg attaaccagg tctttaagac
     8461 gccacttcat cgcattggcc aactctgccg caggtacatc cggcgcttcg agcaaataca
     8521 actgatagcg actttgctgc aacaccaagt tgcacggttt gttttttaat ttattttgct
     8581 taacatagtc acgcagctgc gcaaaacgct gtttttcatc ctcccccggt aaaaaggcaa
     8641 ggcgggctat atggggtatt tggcttttga caacttttac cgctacagct atgccatctg
     8701 cttgcaactc caatccgacg aggctttgag gctgagcctg atttactctt aacaacacgg
     8761 cgtagacccc tttttcatcc tgcgcaagtt ttttgttgtt attttctcaa ttaccggcac
     8821 ataaaattga tatttgacga ttttccttta gtatagccag cactttggat ttcgctcttt
     8881 ttctgcagtt ttacttatat atgattcaca tcgtgttatt cgaacccgaa attccaccca
     8941 atacgggtaa tatcattcgg ctatgtgcca atacgggatg ccaactgcat ttaattgaac
     9001 cctttgggtt tgcactggac gataaaaaac tgcggcgagc cggattagac tatgccgagt
     9061 ggaaagacat taaaatttat aaggattggg aggtgttttt gcgggagtta gcgccgcccc
     9121 gctggttcgc gatcaccaca aagggctcgc agaattacac caaggtggca ttcgcagcgg
     9181 gcgatgcctt ggtatttggc ccggaatcgc gcggcttgcc tccggcaata ttagacagcg
     9241 cagcgccgga gcggcgcttg cgaattccca tgcggccggg cagccgcagc atgaatctat
     9301 ccaacgccgc ttccgttatg gtctatgagg cctggcggca attggatttt aacggcgcct
     9361 tgtaaaacac tatgttcaaa gcatggcact tgaggcatta attaggcttt ttctccccac
     9421 ccatacaacg cggcgaggca ggcgaatccc cctgccattc atccggcgcg taggtatgta
     9481 gtgccagcgc atgtacaccg ccgctgagct cctcttttaa tactgcataa acagcgcggt
     9541 ggcgggccac ggaggttaaa ccgctaaagg cgtcactcac caaagtaact ttaaaatgcg
     9601 tgttattgcc cggcctgctg ctgtggtgat ggctctcatt ttcaacctgt aaaacactgg
     9661 gtgaaaacgc cgcgtggagt ttttgctgga ttgcgtgttc ggtcataggg tctttctcac
     9721 gatctggttt aataggacaa aaattttatc caggccattt tctcagctag catttatgat
     9781 gaaagcggcc tgtgatgagc tgttgagtcc attcgtattt ggggatgtta tgagttcgag
     9841 cattatattt gttaatcgcc gcaaaggccg agaccgccgt caggacgccg atccctgcaa
     9901 agaaatgccc ctggatctat tccatcgcaa acgccgcaaa tccaaagagc gccgcgacac
     9961 cagccgcacc cttacggacg actactacgc ctctatgcaa aagaccttgg ccagcttaca
    10021 aaacgagcaa actggcaagc cctagcctac tgcacagcca aggccaattc gtcaggaatc
    10081 gactcatttc atacccggag gtgcgcgtga gcgaggtaca taatgtcgct gcttgacccc
    10141 aaagagctta aattggtggt cactctgctg cagcgacaac gcatggcaat ggccaaaacc
    10201 caggcaactc tgcacgatga aaaacagagc gccgaattag ccgccaccct gaagctgctg
    10261 gacggtgcgc tgaacaaact tgttgcaagc ggtacaaaat ccgtcgcaaa ccccacaccc
    10321 agcccgctgc cagccaaaaa gcctattaaa cgcagagaac ccattccttt agctgatgca
    10381 ctggtactcg tcgcggacga cagcatcgat tccatgatga tgttgcgggg tattttgagt
    10441 gatctgggca tcaaaaaaat agacagcgtc aaggacggcc gcgag
//
LOCUS       DAOQGS010000072        10478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2966_length_10478_cov_5.004701, whole genome shotgun sequence.
ACCESSION   DAOQGS010000072 DAOQGS010000000
VERSION     DAOQGS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2966_length_10478_cov_5.004701"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PKZ52_04210"
     CDS             complement(<1..1087)
                     /locus_tag="PKZ52_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNG59006.1"
                     /translation="MGRVFFSLYGLILATLVGAGFILDQLVGATDEDALHPAERVATD
                     LAASHSSQLPYTTAEGWTLERLSGADLTDNQTLSELKTKKSLAVASVDSPSGIYYRDV
                     FRYFADNHIIHLRYAVPHAPEPWQYRAILLVVYLFIGLAVFSWLWPLMRDLRRLALQT
                     QQLDFAEAFCGLTLKKTSPLFALSEAFCGMSQRLGSVLASYKEMTYAVSHELRTPLAR
                     MKFALTMAQDQMANCAVPRNVQEQWQSLWLDVNEMDALVNQLLNYASFEQENRALQVQ
                     TENMQDFLADLVQRARKASPHLTISLHCEPCVQVAADWQLMERVMINLLENAARFAAT
                     CIEVRATTPGELIVVSVSDDGPGVPSSE"
     gene            complement(1090..1794)
                     /locus_tag="PKZ52_04215"
     CDS             complement(1090..1794)
                     /locus_tag="PKZ52_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG59007.1"
                     /translation="MTTATPCQILLVEDDLRLANLIAEYLTGQGFSVHTETSGLRVAR
                     ITADICPDLIILDISLPGKNGLQICQDIRGDYQGPIMFLTARTENAEQIQGLESGADD
                     YVLKPVEPQLLLARIKALLRRYGSDAAAAQQEICLGALQINLKLRTASRAGEALSLSS
                     HEFDLLVTFAKHAGQVLSREYLFSRVYSRPYDGLDRTIDVRISQLRKKLGETPDAAHI
                     IKTIWGRGYLLVADAL"
     gene            complement(1794..2144)
                     /locus_tag="PKZ52_04220"
     CDS             complement(1794..2144)
                     /locus_tag="PKZ52_04220"
                     /inference="COORDINATES: protein motif:HMM:NF022895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3019 domain-containing protein"
                     /protein_id="HNG59008.1"
                     /translation="MGFATTAAALEHNIELTLTPRLCVLGEAEQECGGTVTLRWQAPA
                     ALNTCLYQEGRAEPLHCWQQVALGEFTLAPKANRSLRFYLAAANGSILADGTFQVLQQ
                     QNAHRKRRNPWSFY"
     gene            complement(2185..3054)
                     /locus_tag="PKZ52_04225"
     CDS             complement(2185..3054)
                     /locus_tag="PKZ52_04225"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HNG59009.1"
                     /translation="MRNLAVRYLLLISLLGQSLAAAAGAPAKTLCQAPCTSEGGWELS
                     VGLGLGMSTNPLKGGSDIPLLVRPRLSYYGERVFLDNYEAGVMLWENSSQQINLLVLP
                     SLEQLYFRRWQYASGGFSLDPDFIKGEGAAESTGDKPPHLHHRHTSALGGFEYNASLG
                     GFFDGLEFNWQFLEELSGYHAGREMRLAISRDFLTYWRASLGANFQSRDYINYYFGVS
                     ASEASPLFSRYTPNSGGWSKLVRVEYLKPLSPHLAIKGGAALKIFSSAVADSPLLEES
                     SVYSLFLGGVYYF"
     gene            complement(3199..4305)
                     /locus_tag="PKZ52_04230"
     CDS             complement(3199..4305)
                     /locus_tag="PKZ52_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59010.1"
                     /translation="MTHLVAKTIALTALSVALTACGTGKDASAKTTNTQENVVDQRAV
                     GITEDNLETVLGTAFEITAQASPGGNESNNYPPYADGNLGDGLANIGNAQANSRIADT
                     ATGIDLTTTKANCNAGGTASGAVGLDTANLGANGSTDFLKLYVDFSFNECKTAQFWLN
                     GKATVSLSSQSGILSNNNDSTLLIKLGADLKRFYVAQPTKNGKQAGRSLDITGKVEFS
                     ISNPERKYIRAELNGVPGSSLIYQEKGKSDQLVQLNEFESYLQSSMLDASYSIGVKGN
                     MLSMGKATANSKSLPTSRLKIYTEKTITGQGFAVPRDGVLVVESDKEIARFEILNQVD
                     LKYTLDKGKDGTIDKQVDTTWSEFISQNTGLKFD"
     gene            complement(4515..5228)
                     /locus_tag="PKZ52_04235"
     CDS             complement(4515..5228)
                     /locus_tag="PKZ52_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HNG59011.1"
                     /translation="MPKHAYHSLRQMRLERATKPFNARGARLIRCDTCLLAQSHCICA
                     HTTPMQCGVDWVLLMHPDEVLKPTNTGRLLVALFPAATHVFEWSRVDPPADLLTLIAD
                     PGRLNLLVFPTDDALAPAQAQHQAQQNSQRLTLILLDGTWKQARKMFNRTAWLAGLPS
                     VRLPLSASRYALRKAAHDGCLSTAEAAIGLLDCLDMPAANALYSYFGLFNEAYVHSRG
                     VRPTSPTYTDSMSARDGSD"
     gene            5291..5749
                     /locus_tag="PKZ52_04240"
     CDS             5291..5749
                     /locus_tag="PKZ52_04240"
                     /inference="COORDINATES: protein motif:HMM:NF036804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HNG59012.1"
                     /translation="MPTTKSLCPCGSQQPLENCCARFWRGQPICAPEELMRARYSAFA
                     LGQVEFLMATLHPSRHQPDELTQLNKSLANTQWLALHVLAAEGNSVEFTAFFRQQNKL
                     GQLHERSRFVFEEGRWWYTDGDFLPPLKWERNQMCWCGSGKKYKKCHACD"
     gene            5816..6082
                     /locus_tag="PKZ52_04245"
     CDS             5816..6082
                     /locus_tag="PKZ52_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNG59013.1"
                     /translation="MKATLTQELNMIENHSLALELPEYKDTIHRLKLSDAHFAKLFDE
                     YHATDKEIHRIEQGAEVSSDDYIGNLKKQRLHIKDTLFSMLRSA"
     gene            6666..7463
                     /locus_tag="PKZ52_04250"
     CDS             6666..7463
                     /locus_tag="PKZ52_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNG59014.1"
                     /translation="MGKKSHAGRHHPTYHGDPSYETIHPKSMRKRNRLDDNIVEIRSR
                     SQQVETAYSPPRSTQPFRARTPSQQRYINAINNHMLTFGIGPAGTGKSYCAGALAAEA
                     LESGRIERIILTRPAVESGESLGFLPGDLDQKFSVYIDAFRDILNERLGAGTVDYCLR
                     HGRIVAAPLAFMRGKTFNSKTFVILDEAQNTSPAQMKMFLTRIGEDCKVVINGDIQQS
                     DIKGPNGLADAVVRLGHLENVYIHEFERRDIVRSGLVRDILDSYELA"
     gene            8533..8609
                     /locus_tag="PKZ52_04255"
     tRNA            8533..8609
                     /locus_tag="PKZ52_04255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8567..8569,aa:Arg,seq:tcg)
     gene            8687..9652
                     /locus_tag="PKZ52_04260"
     CDS             8687..9652
                     /locus_tag="PKZ52_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNG59015.1"
                     /translation="MNKLASLQRWLKATRHWLYPLIALALAALVYEGVVKLLKKLDLN
                     QVWLSIQDTPPHSLVLALAATAVSYVALMGYDFGSLRLVGAKVPAKKIAKTAFMAYAL
                     GNSLGAGMLTGGALRMRHYMAEGASAGQVTRAIVINALSFGLGIGVLGAMGILWQSDK
                     VEEVIHLPALLLQILAVVFLLSLGSWGFFGHRLQGLKLFGRELPLHPGHRFLSLQLAV
                     SCLDIVASGFALWILLPPGAVSFELFIVFFAIAITLGVLSHVPGGLGVFEALMLLCIG
                     KDVGPEALTAALLVFRGLYFLLPLVLAFLLIALEEWWMARRADKA"
     gene            complement(9673..>10478)
                     /locus_tag="PKZ52_04265"
     CDS             complement(9673..>10478)
                     /locus_tag="PKZ52_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59016.1"
                     /translation="QSPLSFDLQGDMRSIKLANYASYFAALYQESPKEGELDGTITIS
                     SAANLFKLEGNVVLSELRTVGDGNLARQGLDMTLGRAFERLRGESQQVTLDWRFTTDL
                     GKDKDPLKTALAKSFKNTLLRMAQNDWRSAGVTNLAGLGNNPEGPLSFDSFRYSPHER
                     DLSPDQERRLKDLAALVNRRPKEKLKLCAIATSVEWSALYNHGAPLVPGSLVADDQLQ
                     YLIDLSAARVRSIKVQLGELGIGSQRFISCEPKVEMGAKMMSYVSVEMI"
BASE COUNT         2427 a         2632 c         2804 g         2615 t
ORIGIN      
        1 gttcgctgct gggcacaccg gggccgtcgt cgctgacgct gacaacaatt aattcgccgg
       61 gcgtggtggc gcgcacctca atacaagtgg cggcaaaccg cgcggcgttt tccaataaat
      121 taatcatcac ccgctccatt aactgccaat ccgccgctac ctgtacacag ggttcgcaat
      181 gcaacgaaat ggttaagtgc gggctggcct tgcgggcgcg ctggactaaa tcagcgagaa
      241 aatcctgcat attttccgtc tgtacttgta aagcgcgatt ttcctgctcg aagctggcgt
      301 aatttaataa ttgattcacc agcgcatcca tttcatttac atccagccaa aggctctgcc
      361 actgttcctg cacattgcgt ggcacggcgc agttagccat ttgatcttgc gccatggtca
      421 gcgcgaattt catgcgcgca aggggggtgc gcaattcgtg ggataccgca taggtcattt
      481 ctttgtagga ggccaacacc gaacccagcc gctggctcat gccgcaaaac gcctccgata
      541 gcgcaaacaa aggcgaggtt tttttaagtg tcaggccgca aaacgcctcg gcaaaatcga
      601 gctgctgagt ctgcaaggct aggcggcgta aatcccgcat caagggccac agccagctaa
      661 aaacggccag gccaataaat aaatacacca ctaataaaat cgctcggtat tgccagggct
      721 ccggtgcgtg gggcacggca tagcgcaggt gaataatatg gttgtcggca aaatagcgaa
      781 aaacatcgcg ataataaatt ccgcttgggc tatccacact ggcaacggca aggctttttt
      841 tggtttttaa ttcgcttaag gtttggttgt cggtcaggtc ggcgccgctt aagcgctcaa
      901 gtgtccagcc ctcggcggtg gtgtagggta attggctgct gtgactagcg gccaaatccg
      961 tggccactcg ttctgccggg tgcaaggcat cttcatcggt ggcgcccact aactgatcga
     1021 gaataaaacc tgcgcccacc aaggtggcca gaattaaccc gtagagggaa aaaaacacgc
     1081 gccccatggt tacaatgcgt cggctaccaa taaatagccg cgcccccaaa tggttttaat
     1141 gatgtgggct gcatccggtg tttcgcccaa ttttttgcgc agttgcgaaa tgcgtacgtc
     1201 gatggtgcga tccaagccat cgtagggacg ggaataaacc cggctaaata aatattcgcg
     1261 gctgagcacc tggcctgcgt gtttggcaaa ggtgactaat aaatcaaatt catggctgga
     1321 taaacttaag gcttcaccgg cgcggcttgc tgtgcgcagc tttagattaa tctgcagagc
     1381 gcctaagcaa atctcctgct gcgcggcggc ggcgtcgctg ccgtagcggc gcaataaggc
     1441 tttaatacgg gctaataata gctgcggttc caccggtttt aacacgtagt cgtcagcgcc
     1501 ggattcaagg ccctggattt gttcggcgtt ttccgtacgg gcggttaaaa acataattgg
     1561 cccttggtag tcaccgcgaa tatcctggca aatctgcagg ccgtttttac ccggcaaact
     1621 aatgtcgaga ataattaaat ctgggcaaat atccgccgtt atacgtgcaa ccctaagccc
     1681 cgacgtttcc gtatgcacac taaaaccctg gccggttaaa tactcggcaa ttaaattggc
     1741 caggcgcagg tcatcttcca ccaataatat ttggcagggg gtagcggttg tcattaataa
     1801 aagctccaag gattgcgccg tttgcggtgg gcgttttgtt gttgcaacac ttgaaatgtg
     1861 ccatcggcca aaatactgcc attggcggcc gctaaataaa atcgcaggct gcgattggct
     1921 ttgggggcca gagtgaattc accgagggcg acttgttgcc agcagtgtaa aggctccgcg
     1981 cggccttctt gatacaaaca ggtattaagc gccgcaggtg cctgccagcg cagcgtaacc
     2041 gtgccgccac attcctgttc ggcttcgccc aacacacaca ggcgcggtgt gagggttagt
     2101 tcgatgttgt gctccagtgc ggctgctgta gtggcgaaac ccaaaacact cagcgcaaca
     2161 aggcccctgt gcgcaaggag atgtttaaaa ataataaaca cctcctaaaa aaagactgta
     2221 taccgatgat tcttccagca gcgggctgtc ggccaccgcc gatgaaaaga tttttaatgc
     2281 ggcgccacct ttaatggcca ggtgtggact cagcggtttt aaatattcca cccgcacaag
     2341 tttggaccag ccgccgctat tgggcgtgta tcggctaaat aatgggctcg cctcactggc
     2401 gctaacgccg aaataataat taatgtagtc tcggctttga aaattcgccc ccagacttgc
     2461 gcgccagtag gttaaaaaat ctcggctgat ggccaggcgc atttctcggc ctgcgtggta
     2521 accggataat tcctccaaaa attgccagtt aaattccagc ccgtcaaaaa aaccacctag
     2581 ggaggcgttg tattcaaaac cacccagagc actggtgtgg cggtggtgta gatggggagg
     2641 cttatcgcct gtggactctg ccgcaccctc acccttgata aaatcaggat ccaaagaaaa
     2701 accgccggaa gcgtattgcc agcggcgaaa atacaattgc tctaaactcg gcagcaccaa
     2761 taaattaatt tgttgcgacg aattttccca cagcataacc ccggcttcat aattatcgag
     2821 aaatacccgt tcgccgtagt agctcaagcg ggggcgcact agcagaggaa tatcactgcc
     2881 acctttgagc gggttggtgc tcatgccgag gccgaggccg acactcagct cccagccccc
     2941 ttcgcttgtg cagggcgctt ggcacaatgt tttggcgggc gcccctgctg cagccgctag
     3001 gctttgccct aacagcgata tcagtaataa ataacgaaca gctaaattgc gcacacagca
     3061 ggctcaataa aaaggcgaat gcgcagaatg ccggtgcggg agggtttttg taagggcatt
     3121 tttggtaata aattgtaacc aaggcttcaa aacgcaaaaa gccatccgag gatggctttt
     3181 tgcagagctg gcttttgatt agtcgaattt aaggccagta ttttggctga tgaactcaga
     3241 ccaggtagta tcaacttgtt tgtcaatagt gccatctttg cccttatcta gggtgtattt
     3301 cagatcaact tggttaagga tttcaaagcg ggcaatctct ttgtcgcttt caaccaccaa
     3361 tacaccatct ctgggaactg caaagccctg acccgtaata gtcttttcgg tgtaaatctt
     3421 taaacgagag gtgggcaggc ttttgctgtt ggcggtggct ttgcccatgc tgagcatgtt
     3481 gcctttaacg cctatggagt agcttgcatc taacatgctt gattgcagat aggattcgaa
     3541 ctcattgagc tgtaccagct ggtcggactt acccttttct tgatagatca gcgatgaacc
     3601 aggcacacca tttaactcgg cacggatgta tttgcgctca gggtttgaaa tcgaaaactc
     3661 cactttacct gtaatatcca agcttcgacc tgcctgctta ccgtttttag ttggctgggc
     3721 aacataaaaa cgttttaagt cggcgccgag cttaattaaa agagtgctgt cgttgttgtt
     3781 ggagagaata ccggactgag aagagagaga cacggtcgcc ttaccattca gccagaactg
     3841 cgcggtctta cactcgttga agctgaaatc cacataaagc tttaaaaaat ctgtagagcc
     3901 attggcgcct aagttcgcgg tgtctaatcc caccgcacca ctggcggtgc cgcctgcatt
     3961 acagtttgct ttggtggtgg taaggtcaat accggtggcg gtgtcagcta tgcggctgtt
     4021 ggcttgagca ttacctatat tggcaaggcc atcaccgagg ttgccatccg catatggagg
     4081 atagttattc gactcattgc cgccggggct ggcttgagca gtgatctcaa atgcggttcc
     4141 caacacagtt tccaggttgt cttctgtaat ccccacggcg cgttgatcga caacattttc
     4201 ttgggtattg gtggttttcg cgctggcgtc tttaccagta ccacaagcgg ttaaagcaac
     4261 gctcaatgca gtcagagcga tggtttttgc aactagatga gtcatgcctc gtctccatgg
     4321 agagaatttt atatcgtgga gcgagtataa gcctttgcgc gtcaataaaa ggcaaagcat
     4381 ttggcatttt ggatggactt acttagcgtt actaacaagt cgctaccgtc ggcctaaggc
     4441 ttacgtcgcg tgcaggcggc agtataccga tctgggtgag tatttactgg cttttttagc
     4501 cgctgtaatc gcggttagtc gctgccatcg cgcgcactca tggagtcggt ataggttggg
     4561 cttgttgggc gcacaccgcg gctgtgtacg taagcctcgt taaacaagcc gaaataactg
     4621 tagagggcgt ttgctgcagg catatctagg caatccagca ggccaatagc cgcttcagca
     4681 gtggataaac agccgtcgtg agccgcttta cgcagggcgt atcgggaggc cgataagggc
     4741 aggcgaacac tgggtaaacc tgcgagccat gcagtgcgat taaacatttt gcgggcttgc
     4801 ttccaagtgc cgtccagcaa aattagcgtc agccgctggc tgttttgttg cgcttgatgc
     4861 tgcgcctggg cgggtgccag agcgtcatcg gtggggaata ccaataggtt tagacgcccc
     4921 gggtcagcga ttagggtcag caaatccgct ggcggatcca cccgcgacca ctcaaataca
     4981 tgggtggcag cggggaagag tgccaccaaa aggcgcccgg tattggtggg ttttaacact
     5041 tcatccgggt gcattaataa aacccaatcc accccacact gcataggggt tgtgtgcgcg
     5101 caaatgcaat ggctttgtgc cagcaggcag gtgtcgcagc gaattaaccg ggcgccacgg
     5161 gcattaaagg gtttggtggc gcgttcaagg cgcatctggc gcaggctatg gtaggcgtgt
     5221 ttaggcatgg caaaatgcgc aagtctaatc gttttgagtg ccgcaacggc atgtgctggg
     5281 gagttttatt gtgcctacca ccaaatcgct gtgcccctgc ggcagccaac agccccttga
     5341 aaattgttgt gcccgcttct ggcgcgggca gccgatttgc gcacctgagg agttaatgcg
     5401 cgcccgttac agtgcctttg cattggggca ggtggagttt ttaatggcga ctttgcaccc
     5461 cagccgtcat cagccagatg aattaacgca attaaacaaa agcctggcta atacccaatg
     5521 gctggcgttg catgtgttgg ctgccgaggg caatagcgtg gaatttaccg ctttttttcg
     5581 ccaacagaat aagctcgggc aattgcacga gcgctctcgt tttgtattcg aagaggggcg
     5641 ctggtggtat acagacggcg attttttgcc gccactaaaa tgggagcgca accagatgtg
     5701 ttggtgcggc agcggcaaaa aatataaaaa atgccacgcc tgcgactaaa aaacgcattt
     5761 gcaaagcagg taaaggttga tatgtgtcaa tgagccgttg cggtaggttt gcacaatgaa
     5821 agcaacactc acccaggagc ttaatatgat tgagaaccac agcctcgccc ttgaattacc
     5881 cgaatacaag gacactattc atcgtttgaa attaagcgat gctcatttcg caaaattatt
     5941 tgatgagtat cacgcaacgg ataaagaaat tcaccgcata gagcaggggg ctgaagtttc
     6001 ctcggacgac tatattggaa acttaaaaaa gcagcgctta catatcaaag atacactctt
     6061 ctccatgttg cgcagcgcct gaccaaatgc gggttagttc ttatttgaaa tcctggcagg
     6121 aaataggttt gggggtttct gtaagtgcat ccctaaaatt cgcaggccca ggcagatttt
     6181 gtttggggct gcgcgtgaat tttattagcc gctacgaatg ccccgcctgc tacagcctat
     6241 taacgcctct ctctcgtccc tatctgctgc ctgccttata acctagtcct taaactttgg
     6301 tttaaaaata aatgcgcaat ctattggaca agcggcattg aatcgaccta atatcagacg
     6361 gaagggagtt aagttgtatc gccccaggca cagggttaat gcctgtatgc aggttccttc
     6421 tgcatagtgt gtaaagtgtt gtatggagca ggcggaacag tttgtgcaga taagtttctg
     6481 tttgtacgcg gccgctgtaa tacacctgca atgggcgctg ggtaaattgt gttgggctag
     6541 tttcaataaa ccaaattgtc ttaagggggt taaatcaagg tttacatttg agctgacagc
     6601 tcagcttaaa tcgaggctcg ttttgctttt caactaccac attcacctct aggggacatg
     6661 aatttatggg taaaaaatcg catgccggac gccaccatcc aacgtatcac ggcgatccga
     6721 gttacgaaac catacatcca aaatcaatgc gcaaacgcaa cagacttgac gataacatag
     6781 tcgaaattag atcccgctct caacaagtcg aaaccgccta ctcccctcct cgctctaccc
     6841 aacccttccg cgctagaact ccctcccaac aacgctacat caacgccatt aataatcaca
     6901 tgctgacatt cggaattggc cctgccggta ccggtaaaag ttattgtgct ggcgcattgg
     6961 cggctgaagc gctggaatcg gggcgcattg agcgcattat tttaactcgc ccggcggtgg
     7021 aatccggtga aagcctcgga tttttgcccg gcgatttaga tcagaaattt tcggtatata
     7081 tcgatgcctt tcgcgatatt ttaaatgagc gcttgggcgc aggtaccgtg gattattgtt
     7141 tgcgccacgg ccgtattgtg gctgcaccct tggcatttat gcgcggtaaa acctttaata
     7201 gcaaaacctt tgtgattctc gacgaggcgc aaaatacctc gcctgcgcaa atgaaaatgt
     7261 ttttaacgcg cattggtgag gattgcaaag tggtgattaa cggtgatatt cagcagagcg
     7321 atattaaagg tcccaatggc ttggccgatg cggtggtacg cctagggcac ctggaaaatg
     7381 tgtatatcca tgaatttgag cgccgcgata tagtgcgcag cggtttggtg cgggatattc
     7441 ttgatagcta cgagctcgcc taaaaggccg cccttgggtg gcattttaat agcctgaccc
     7501 ggtgagataa ttgccgggtt tttagctgaa ttttttagcc ccgtggcaag atttttttgt
     7561 gcgggtgttt tttgcggtgc gtgtgattgc atcgcaagat ttcttatccc ctaaaaaagc
     7621 gaacatgcct ttaggcgata gttaaagttt tttaactatt cagcttagct tctttttatt
     7681 gtttctctta attgtattca attgacataa ttcccccgct gacagaaaaa cctcaggcga
     7741 gtgctacggc tgcgagccct taatacttag cccgcagtgg tgaatacaga ttgttgcaga
     7801 cacgccgtga atacatccgt gtaggcgcgt caaaatggtc cgtctttttg acggcctgta
     7861 ataacctgta ttcaccactg ctgcaccgcg aatcaattct accgctcggc taaatcctcc
     7921 aaaatttatt gtccgcgcgg aatagggtag taaagtgtgc tgattaaggt gtgctgatta
     7981 aggtgtgctg attaaggtgt gctgattagg gtgtgctgta tagctgcaag actttaagat
     8041 tttcgtagcg ctatttgggc gctcgctccg attataaaaa tctcgccaag gtatttctgt
     8101 tcgcgcggca atacaaagga ttattcttaa gccaggtgaa cgcctattgt gaacactcaa
     8161 gcgggagctt ccggctgagg gtagttttgt ccagataaaa agacggtaaa cgcagcggtt
     8221 ttccaaaagg ctggagccaa aaggttagag ccaaaagttt ggagtcaaaa cgttaaggta
     8281 tggtccgtca atttccgaag cctgctccac agggcaagtt cataatggcg tagttgcatc
     8341 gatcaacttg cccgcgcact ttccagcacc cagtcagtac accttggcgc ttagaaacta
     8401 ttcaattaaa gccaagccca gggctgtgct acctaggcgc cattatcgct cggggcagcc
     8461 ctagaggact ggttttactc gtaaaaaagt tttgcccaaa ttcaattttc ccgccatact
     8521 tcgcggcctt acgcacccgt agctcagctg gatagagtag gtggcttcga accacttggt
     8581 cgggagttcg aatctctccg ggtgcgccat atctttaaag ccctctttgc gagggctttt
     8641 ttttaggcat tgcgcctttg tgactaggtt ttatgcgcgg gtctcaatga acaaactggc
     8701 atccttgcag cgttggctta aagcgactcg gcactggctt tacccattga tagcacttgc
     8761 gctggccgcc ctagtgtatg aaggggtggt gaagcttttg aaaaagctcg atctgaacca
     8821 ggtttggctg tccatccaag atacgccccc ccactccctg gttttggcgc tggccgccac
     8881 tgcggttagt tacgttgccc taatgggcta cgactttggc agcctgcgtt tggtgggcgc
     8941 caaggttccc gctaaaaaaa tcgctaaaac cgcctttatg gcctatgccc tcggcaacag
     9001 cttaggggcg ggtatgctca ctggcggcgc gctgcgcatg cgccactata tggcggaagg
     9061 cgccagcgca gggcaggtga cgcgagcaat tgtcatcaac gcgctgagtt ttggtttggg
     9121 cattggtgtg ctcggcgcca tggggattct ctggcagagc gataaggtgg aagaggttat
     9181 tcacctgccg gcgctgctgc tgcagatact ggccgtggtg tttctgctca gcctcggctc
     9241 ctgggggttt tttggccatc ggctgcaggg gctcaagctg tttggcaggg agctgcccct
     9301 gcaccccggc caccggtttt tatccctgca gttggccgtg tcctgcctgg atatagtggc
     9361 ttcaggcttc gcgctgtgga tcttgctgcc gccgggggcg gtcagttttg agctgtttat
     9421 tgtgtttttt gccattgcca tcactctggg cgtgctcagc catgtgcccg gcggtttagg
     9481 ggtgtttgag gccctcatgc tgctgtgcat cggcaaggat gtgggcccgg aggccctcac
     9541 tgcggcactg ctggtgttcc gcggcctgta tttcctgctg ccgctggtgc tggcgttttt
     9601 gttgatcgcc ctggaagagt ggtggatggc gcgccgcgcc gacaaggcct agggttgccg
     9661 acggcctgag tgttaaatca tttcaacgct gacgtaggac atcatcttag cgcccatctc
     9721 caccttgggc tcgcagctga taaagcgctg gctgccaatg cccagctccc ccagttgtac
     9781 tttgatactg cgcacccgcg ccgccgacag gtcgatgagg tattgcagct ggtcgtccgc
     9841 cactaggctg cctggcacaa ggggggcgcc gtggttgtag agggccgacc actccaccga
     9901 ggtggcaatc gcacagagct tcagtttttc tttcgggcga cggttcacca gggcggctaa
     9961 gtctttcaga cggcgctcct gatcgggcga gaggtcccgc tcgtggggac tgtagcggaa
    10021 ggaatcaaaa ctcagtggcc cctccgggtt gttgcccagg cccgccaaat tcgtcacccc
    10081 cgccgagcgc caatcgtttt gcgccatgcg cagcaaggtg tttttaaaac tcttcgccag
    10141 ggcggttttg agcgggtctt tatccttgcc gagatccgtt gtaaagcgcc aatcgagggt
    10201 gacctgctgg ctctcgccgc gcagccgctc aaaggcgcgg cccaaggtca tatccagccc
    10261 ctggcgggct aagttgccat cccccacggt gcgcagctca gagagaacaa cattaccctc
    10321 taacttaaac aagttcgcag cactgctgat ggtgatggtg ccatccagct cgccctcctt
    10381 cgggctctcc tggtacaagg cggcgaaata actggcgtaa ttggccagtt tgatactgcg
    10441 catatcgccc tgcagatcaa aggacagggg gctttgga
//
LOCUS       DAOQGS010000073        10469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2970_length_10469_cov_4.730459, whole genome shotgun sequence.
ACCESSION   DAOQGS010000073 DAOQGS010000000
VERSION     DAOQGS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10469
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2970_length_10469_cov_4.730459"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKZ52_04270"
     CDS             <1..344
                     /locus_tag="PKZ52_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HNG59017.1"
                     /translation="LQALTRDHSRVQELADQGRPDPHAVGANIITRALGVDEFLPMDM
                     SLFSAQHGDTFLLCSDGLYNVLDEATLIKGLSLETNQLACQYFLDTALQIGANDNVSA
                     VVLRAVPWSSP"
     gene            341..550
                     /locus_tag="PKZ52_04275"
     CDS             341..550
                     /locus_tag="PKZ52_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59018.1"
                     /translation="MSQPSFADLLKDLAAQYNAGRLTFEQFRSLRRALLRDAEAQYLA
                     PPPPPFGDHYTPPANDDITVVPPPG"
     gene            complement(769..1959)
                     /locus_tag="PKZ52_04280"
     CDS             complement(769..1959)
                     /locus_tag="PKZ52_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59019.1"
                     /translation="MTAIRGQCLLVVACYMASAGAIGEAPTGADLPLPQACVSGFTSQ
                     DTASSSGQISAEDNTSNQLPPRVSQWLRAQHWQAGYQLWQRCRSGLAGPEAAACAGLP
                     AQERICQGPQDGLAFLAMHRHILQSLRAAWPSYRDELASWRRFPEGSDYPADLQAAFT
                     PWPSATARAAQAVDNLRSLPRAQLLARWPSEGAFGQWLQCGSGAGGLAVDSLYGALLG
                     NSGLELSSHRFHSSVYWRYLAWIDRAWDAYRAKLGKTPDDPQLQALLIQQCQQFTALA
                     QRASQLNPPPQPPAQPLPPLFVAGALNPNRASSWVSVLGEVQRVTSGSAGQVLIQLDM
                     HLRGAQPLWLASTTALAGIQSGQRLRIGGYLQPAASLDSSGQLAARTQAVYLVQVESL
                     QTIQ"
     gene            complement(1966..3468)
                     /locus_tag="PKZ52_04285"
     CDS             complement(1966..3468)
                     /locus_tag="PKZ52_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59020.1"
                     /translation="MKIHPGSADPRSLVGIGIVTLALAACTGTPAGSSSSSSSSSSSS
                     SSSSSSSSSASSSGRSSSSSSSSSSSSGRASSSSSSSSSSGVTSVDHCLTGFKPHFTN
                     GHIGSSYFEHTLNGQTDVSVQPAVYDYMYDNGWQDAHVLWHQARTCGGFGGANGINGL
                     PSACNFKDLLPKQNDCQGAANGLDFLSGHRLMRQQLIELWPDNAEQFTGWSKFPRTAA
                     DFPPAVRPYFKAWGSEILKAADIADNIEQHLSEFPTEGDLGTWISCAIKPGGGSAADF
                     STARNLHFALHNNGVPVNNQKHAANNNNVNIDAYLFWKLHGWIDNVWDRYRAAKNKTA
                     NDADYKAEMLEQCREMDKWRQIGVDTRGSKGHPNQDADFVPNVKESGFFHEKIRPALE
                     SQAANCASCHGAGEEAGLRLGYQITSTEIVEKLIDRPSAYAKDYKLVVPGNPEKSWLY
                     LKASGLSRDSGVVCQGNASCSKVMPGLSGQNIQDLYDWIKSGAPAPTILP"
     gene            complement(4151..4747)
                     /locus_tag="PKZ52_04290"
     CDS             complement(4151..4747)
                     /locus_tag="PKZ52_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNG59021.1"
                     /translation="MTAYVLLPGYGNSLDGHWQELWLGQLSNSHWAELGGTGAEESAP
                     EALGSWERPDRALWVARLQRLVNSLDEPVVLISHSLGGLTIAHWAAEHPSTCSKILGA
                     FMVAVPDPQRADFPSAISGYTNPPQAPLPFPSLMVTSSDDPYASIARSTEFAAAWGAQ
                     TWDIGKAGHINVARGFGPWPEGLTALARWQASLARAQK"
     gene            complement(4758..5018)
                     /locus_tag="PKZ52_04295"
     CDS             complement(4758..5018)
                     /locus_tag="PKZ52_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNG59022.1"
                     /translation="MSVTLAPSDSLAKLKTIIVTHLKRLESADQLPEDAELASLGLDS
                     MTAMNLLFDIEDQFGVAFDESLLTPEVFKTCRSLHNALVTLI"
     gene            complement(5038..6285)
                     /locus_tag="PKZ52_04300"
     CDS             complement(5038..6285)
                     /locus_tag="PKZ52_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59023.1"
                     /translation="MNTPYQLDFHRISQLAERFGTPLYLYELTQLEAAIARLKAALPA
                     GATLYHSLKSNPLPALLQASVAQGCKPEICSTGELAVALASGCAAEHFLYSGPGKSPG
                     ELRAALKAGVRQFSVESWGDLTRLKQQAEAFGGGELRVLLRVNPAEGIQAGLAMSGSA
                     TQFGFEEADVIAGKGRLAELGPHLTPIGFHVYYGTQIPNEDSLLASFTSAIACCERLS
                     EALNLRPQVLDLGGGFPWPYASAGEGADFSQLAERLTPVLAGRNWTQNAALWFESGRY
                     ITAACGTWVSRVLDVKTGKDGQRFVVMDSGINHLGGMAGLGRVVRPYTGFISGRATDD
                     ADKAAVVGPLCSPLDRLASQAPLGDVQVYDLLAIPNAGAYGLTASLTGFLSHPAPVEL
                     VLRRGEAQGVFQLRSGHESLNLL"
     gene            complement(6300..7778)
                     /locus_tag="PKZ52_04305"
     CDS             complement(6300..7778)
                     /locus_tag="PKZ52_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNG59024.1"
                     /translation="MRTLFTRFSQAAEQFGSAPYLKNDFQQVSYQAAFGLVCSLAQQM
                     AASGVGQWDRVLLVCPNHIHVPIAVLAINALGAIAVVVNDQTTQASLNNILKETQPKL
                     IFGHTSQALRWAEQSDWPVWPIDTLSLTPTSTLPVVAPACSQDPALMIYTSGSTGKPK
                     GVVLSQDNVLFASEQIQARLQYRSDDTIGLFLPLSFDYGLYQTFLAFLSGACLYVSDA
                     SKAGPALAGTLQQQGVTVFPGVPHLYNLLTQFLIRKKQMLAGIRLCTNTGAHLPASQI
                     ELLQQWLPNCQVAPMYGLTECKRVAILRPEEVASKPGSVGRPLDDTCAYIVDDAGNLL
                     PPGEVGELVIVGRHVGLGYWQAPEETALRYRQHPCGIGRALFSGDNFRQDAEGYLYYV
                     GRRDEQIKRQGFRINRLEIEDTALRLPGLINACLIQSDNRLVLFVGFMQESMAISERE
                     ILAFLSRELEPYKVPDAIEVLEQFPVSSNGKINRKAIEAAFV"
     gene            complement(7787..8713)
                     /locus_tag="PKZ52_04310"
     CDS             complement(7787..8713)
                     /locus_tag="PKZ52_04310"
                     /inference="COORDINATES: protein motif:HMM:NF023015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HNG59025.1"
                     /translation="MFIKPDVMAVAQKLTSSSLKNSFDTFNRVDYQSKLNPDALQFPE
                     EFLSLYHHPIYGDLTDEQKWKLSLLETVNFFSANIFGEQHLISHMEKKIYRNKFVGED
                     AISSEYAQHFIHEENSHTFMLAGYCVRYHGGVMPNRSIAMADPKLSPYGEDLLFFART
                     YVLEMFLGYMNRLARERDDIDDTVRQIHYFHLLDEVRHIAWDRAMVEGNLWEMRERGL
                     EHEIPQIKELVKTYAKVSFNSLYNPRIYKDIGLENPTRLAMEAAATDQRQQQVDKWSQ
                     GFENYLCKIGLYGAEDLRQPYCAKAAQHTADA"
     gene            9126..9380
                     /locus_tag="PKZ52_04315"
     CDS             9126..9380
                     /locus_tag="PKZ52_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59026.1"
                     /translation="MLLLRELSAIVLLMVFLTLSASCIFIEFSLRNAIIAACCLLAAF
                     LIWPSKRRGQRQDDFWLFDLAELLIEGPVLLISALAKLFD"
     gene            complement(9648..9926)
                     /locus_tag="PKZ52_04320"
     CDS             complement(9648..9926)
                     /locus_tag="PKZ52_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59027.1"
                     /translation="MWHHLEEKDQEKARQLSFRLGDPITIAIAKGYKDAAVFSRFDME
                     NLVTHFYFSPQAAGIAKSVGAKPCPTPLPADTGNVSIGDSFAFDRLFK"
     gene            complement(9965..10288)
                     /locus_tag="PKZ52_04325"
     CDS             complement(9965..10288)
                     /locus_tag="PKZ52_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59028.1"
                     /translation="MGFFDFLDPVKSTGNWVAASAKRITKEDWVKARNNISPLLSQMV
                     RMRPGTPQAVEAAIYKNEIDSLSKLGVAILVAEAVSINPADISEIQSKISTILKNKGI
                     PQDLI"
BASE COUNT         2156 a         2863 c         3110 g         2340 t
ORIGIN      
        1 aactgcaggc cctcacccgc gaccacagcc gggtgcaaga actggccgac cagggccggc
       61 cagaccccca cgcggtgggc gccaacatca tcacccgggc cctcggtgtg gatgagtttt
      121 tacccatgga tatgagcctg ttcagcgccc agcacggcga caccttttta ctctgcagcg
      181 atgggctcta taacgtctta gacgaagcca ccctcatcaa gggcctgagc cttgagacca
      241 accaactggc ctgccagtat tttttagata ccgccctgca gatcggcgcc aatgacaatg
      301 tgtccgccgt ggtgctgcgg gccgtaccct ggagcagccc atgagccagc caagcttcgc
      361 cgatttgctc aaggatctcg ccgcccaata caacgccggg cgcctaacct tcgaacaatt
      421 ccgctccctg cgccgggccc tactgcgcga cgccgaggcc caatacctgg ccccaccccc
      481 gccgcccttt ggcgatcact acacaccgcc tgccaacgac gatattacgg tggtgccgcc
      541 gccgggttga cggctggcgg attttgatct tttaacctta ggttctgatc atcagaacct
      601 gggctttttc tgacgtttag gcggattcaa caggagtaat tagctcgaag gcacaagcca
      661 ctgagagctg gggctctctg attcgactca cacccagagc attcttttga ttagttccag
      721 tgattagctc caggttcgcc ctttcccgct cgttaatgac aagcaggcct attgaatcgt
      781 ttgtaatgac tccacctgca ccagatacac agcctgcgta cgcgctgcca actggccgct
      841 cgaatcgagc gaagctgccg gctgcaggta gccgccgatg cgcaggcgct ggccgctttg
      901 gatgcccgcc agcgcagtgg tactggcaag ccacaggggt tgggcgccgc gcagatgcat
      961 atccagttgg ataagcacct gccccgccga gccactcgtt accctctgca cctcacctaa
     1021 gacactgacc cagctgctcg ctcggttggg gttgagtgcg cccgccacaa acagcggggg
     1081 caagggctgc gccggtggct gtgggggcgg gtttagttgg ctcgcccgct gggctagagc
     1141 ggtgaactgc tggcactgtt gaatcagcag cgcttgcaac tgcgggtcgt ccggggtttt
     1201 gccgagtttt gcgcggtagg catcccaggc gcgatcaatc caggccaggt agcgccaata
     1261 cacggagctg tggaacctgt gggagcttag ctccaggcct gagttgccga gcagggcgcc
     1321 gtacaggctg tctaccgcaa gtcccccggc accgctgccg cactgcagcc actggccgaa
     1381 ggcgccttcg ctgggccagc gggccagcag ttgggcgcgg ggcaggctgc gcaggttgtc
     1441 caccgcctgg gcggcccgcg ctgtggcgct gggccaggga gtgaaggccg cttgcaggtc
     1501 ggcggggtag tcgctgccct cggggaagcg gcgccagctc gccaattcat cccgatagct
     1561 gggccaggca gcgcgcaggg actgtaaaat atggcggtgc atggccaaga aggccaggcc
     1621 gtcctggggg ccttggcaga tgcgctcctg tgccggtaac ccggcgcagg ccgctgcttc
     1681 agggcctgcc aagccgctgc ggcagcgctg ccagagttga tagcccgcct gccagtgctg
     1741 ggctctgagc cattggctta ccctcggcgg cagctggttg ctggtgttgt cctcggcgga
     1801 tatttgcccg ctgctggagg ctgtatcctg tgacgtgaaa ccactgacac aggcctgggg
     1861 cagaggcaaa tcggcgccgg tgggcgcctc gccaatggct ccggcactgg ccatataaca
     1921 ggccactacc aacaggcact ggcctctgat cgcggtcaac accacctagg gcaggatggt
     1981 gggggccggg gcgccgcttt taatccagtc gtacagatcc tggatgttct ggccgctcaa
     2041 acccggcatc accttagagc aactggcgtt gccctggcac accacaccgc tgtcgcggct
     2101 taggccagag gcttttaagt acagccagct tttctccggg ttgcccggca ccaccagctt
     2161 gtaatccttg gcataggcag agggccggtc gatgagcttc tcgacgattt cggtggaggt
     2221 gatttggtag cccaaacgca ggcccgcctc ttctccggcg ccgtggcagc ttgcacagtt
     2281 ggcggcttga gattccagcg ccgggcggat tttctcgtgg aagaaaccgg actctttcac
     2341 gttcggcaca aagtcggcgt cctggttggg gtggcctttg ctgccgcggg tatccacgcc
     2401 tatctgccgc cacttatcca tctcccgaca ctgctcaagc atctcagcct tgtagtcggc
     2461 gtcgttggcg gttttgtttt tggccgcccg gtaacggtcc cacacgttgt cgatccagcc
     2521 gtgcaatttc cagaacaaat aggcatcgat gttgacgtta ttgttgttgg cggcgtgttt
     2581 ttggttgttc accggcacgc cgttgttgtg gagggcaaag tgcaggttgc gggcggtgga
     2641 gaaatccgcc gcgctgccgc cgccgggttt aatggcgcag gagatccagg tgcccaaatc
     2701 cccctcggtg gggaattcgc tgaggtgctg ctcgatgtta tcggctatat ccgctgcttt
     2761 gagaatctca gagccccaag ccttgaagta ggggcgtacg gcagggggga agtcggcggc
     2821 ggtgcggggg aatttgctcc agccggtgaa ctgctcggcg ttatccggcc agagttcaat
     2881 cagctgttgg cgcatgaggc ggtggccgga taaaaagtcc aggccgttgg cggcgccctg
     2941 acagtcgttt tgtttgggca ataagtcttt gaaattacag gcgctgggca ggccgttaat
     3001 gccgttggcg ccgccaaaac cgccgcaggt gcgggcctgg tgccacagga cgtgggcatc
     3061 ctgccagccg ttgtcgtaca tatagtcgta caccgcaggc tgcaccgata cgtcggtttg
     3121 gccgttgagg gtgtgttcaa agtagctgct gccgatatgg ccgttagtaa agtgcggctt
     3181 aaagcccgtc aggcagtggt ccaccgaggt gacgccggag ctggagctag aactggacga
     3241 gctgctggcg cggccggagc ttgaggagct ggaactagaa ctggatgaac tgcgcccgga
     3301 gctggaagcg cttgagctgg atgaactgga gctagaggat gaggagctgg acgagctgga
     3361 gcttgaggag ctaccagcgg gggtgccggt acaggcggca agtgcgaggg tcactatacc
     3421 tatgcccaca agcgagcgcg ggtcggccga ccctgggtgg attttcataa tgcaagccct
     3481 ataagttttt tgttttttaa tatgtcaaaa gcccggtcgc tctgcggcta ccatcgccct
     3541 aggtgcaaag taaacccctc tctgccacgg gtctggggca tttatcacat atatctttac
     3601 tgtgcgacaa atcgccatac tttgtcggta gaggatgaac tgcatcacag gtgcaaatcc
     3661 ccgtgaatac agagtttttg tcatgccata aaacaaaaag gcttgttttt aaggtttttt
     3721 tggcgggagc cgacgggctg gaatctggtg tgagctggtt ttgggtcgtc tcagggtgga
     3781 tggccgtggc cgcaagcgca cgcctgtgat cgagtatgtg cggttaagag ttaggcgcac
     3841 tggggtaggc gatgggtgtg gatgcgggca aggccaaaca ttggccgtct gtaccggttt
     3901 gcgcggggca atgtcattcg gtttgggaaa ttggcctatc ttgcatggca cttttccctc
     3961 cttagtcctt ccgcccaaac cccctccccg gcctccccct taaaaagggg gaggagtaat
     4021 aaaccttaca ttggggcatt ttgcttggcg aggcgttttg ccccctcccc ttgttaaggg
     4081 gagggctggg gaggggttaa tggaatgcat attgatattg gaactagctg gggagcctgg
     4141 ctggagccac ctacttctga gcgcgggcta gacttgcctg ccagcgcgcc agggcggtga
     4201 ggccctccgg ccaggggccg aagccacggg cgacgttgat gtgccctgcc ttgcctatat
     4261 cccaggtctg cgctccccag gcggcggcga attctgtgct gcgggcgatg gatgcgtagg
     4321 ggtcgtcgct gctggtcacc atcaggcttg ggaagggtaa gggtgcctgg ggtggattgg
     4381 tgtagccgct aatagcactg gggaaatccg cccgctgagg gtcgggcacc gccaccataa
     4441 aggcgccgag gatcttgctg caggtgctgg ggtgctcggc tgcccagtgg gcgatggtaa
     4501 gcccccccag gctgtggctg atgagcacta ccggttcatc caggctgttg acgaggcgct
     4561 gcagacgtgc aacccacagg gcgcggtcgg gccgctccca gctgccgagg gcttcgggtg
     4621 cgctctcttc tgcaccggtt ccgcccaatt cggcccagtg gctattgctc agttgcccca
     4681 gccacagctc ctgccagtgg ccatcgaggg agttgccgta gccggggagt agtacatagg
     4741 cggtcatggg gtgctctcta aatcagggta acaagcgcgt tgtgcaggct gcggcaggtt
     4801 ttaaacactt ccggggtgag cagggattca tcaaaggcca caccgaattg atcttcgata
     4861 tcaaacagca gattcatagc cgtcatggaa tccaggccca ggctggcaag ctctgcatct
     4921 tccggcagct gatccgccga ttcaaggcgc tttaagtggg tgacgataat agttttgagc
     4981 ttagcgaggc tgtcgctggg ggcgagggtt actgacatat aagttcctga aggggtatca
     5041 cagtagattt aaggattcat ggccgctgcg cagttggaaa accccttggg cttctccacg
     5101 gcgcaggacc aattcgaccg gggccgggtg gcttaaaaaa ccggtgaggc tggcggtgag
     5161 accataggcg ccagcgttgg ggatggcgag caaatcatat acctgcacat cccccaaggg
     5221 ggcttgggaa gctaggcgat ctaaggggct gcataggggg cctaccacag cggctttgtc
     5281 agcatcatcg gttgcccgcc cgctgataaa ccccgtatag ggccgcacca cccggcccag
     5341 gcccgccatg ccgcccaaat ggtttatccc tgagtccatc accacaaagc gctggccgtc
     5401 tttgccggtt ttcacatcca gtacccggct tacccaggtg ccacaggcgg cggtgatgta
     5461 gcggccggat tcaaaccaaa gggcggcgtt ttgcgtccag ttgcgccctg ctaacacggg
     5521 tgttaagcgc tcggcaagct gactaaagtc cgccccctcc cccgcgcttg catagggcca
     5581 ggggaagccg ccgcccaagt cgagaacttg cgggcgcagg ttgagggcct cgcttaagcg
     5641 ctcgcagcag gcgatggcgc tggtaaagga ggccaacagg ctgtcttcat tggggatttg
     5701 ggtgccgtag taaacgtgaa agccgatggg ggtaaggtgc gggcccagtt cagccaggcg
     5761 ccctttgcct gcaattacat ccgcctcttc aaaaccaaat tgggtggccg agccactcat
     5821 ggccaggccc gcttgaatgc cttccgccgg gttcacccgc agcaacaccc gcagctcgcc
     5881 gccgccaaag gcctcagcct gttgcttaag gcgggtcaaa tcgccccagg attccaccga
     5941 aaactgccgc accccggcct taagcgccgc ccgcagctcg ccgggggatt tacccggccc
     6001 ggagtacaaa aaatgctccg cagcacagcc gctggccagc gccaccgcaa gctcgccagt
     6061 ggagcagatt tcaggcttac agccctgagc caccgaggcc tgcaacaggg cgggcaaggg
     6121 gttggattta aggctgtggt agagggtggc cccagctggc aaggcggctt ttaggcgcgc
     6181 gatagcagct tcaagctgag tgagttcgta gaggtacaag ggcgtgccaa agcgctcagc
     6241 cagttggctg atgcggtgga aatccagctg gtagggggta ttcataggta caccgccact
     6301 catacaaagg ccgcctcaat ggctttgcgg ttaattttgc cgttgctcga cacggggaat
     6361 tgctccagca cctcaatggc atcgggcact ttatagggct ccagctcacg gcttaaaaag
     6421 gccagaatct cccgctcgct aatcgccatg gactcctgca taaaacccac aaacaacacc
     6481 aggcggttgt cggattgaat caaacaggca ttgatcaagc ccggcaggcg cagggcggtg
     6541 tcttctattt ctaggcggtt gatgcgaaag ccctggcgtt tgatctgctc gtcccggcgg
     6601 cccacgtaat acaaataacc ctcggcgtcc tggcggaagt tgtcgccgga aaacagcgcc
     6661 cggcctatgc cgcaggggtg ttggcggtag cgcagggcgg tctcctccgg tgcctgccaa
     6721 taacccaggc ccacatggcg gcccacaatc accagctccc ccacctcccc tggcggcagc
     6781 aggttgcccg cgtcatccac tatgtaggcg caggtgtcat ccaaggggcg gcccacggag
     6841 ccgggtttac tggccacttc ctctggccgc aaaatcgcca cccgtttgca ctcggtcagc
     6901 ccgtacatgg gggccacctg gcagttgggc agccactgct gcaacagctc aatttgcgag
     6961 gcgggcagat gggcaccggt attggtgcac aggcgaatgc ccgccaacat ttgtttttta
     7021 cggatcaaaa actgcgtgag caggttgtac aggtggggca cacctgggaa taccgtaacg
     7081 ccctgttgct gcaatgtgcc cgccagagct ggccccgcct tagaggcgtc actcacatac
     7141 aggcaggcgc ctgagaggaa ggccaaaaac gtctggtata ggccataatc aaacgacagg
     7201 ggcaaaaaca ggccgatggt gtcgtcgctg cggtactgca ggcgggcctg gatctgctcg
     7261 ctggcgaaca acacattatc ctggcttaac accacaccct taggcttacc cgtggagccg
     7321 gaggtgtaga tcatcagcgc cgggtcttgg ctgcaggcgg gggccaccac aggtaaggtt
     7381 gaggtgggtg taaggcttag ggtgtcgatg ggccacaccg gccagtcact ctgctctgcc
     7441 cagcgcaggg cttggctggt gtggccaaaa attaacttag gctgggtttc cttaaggata
     7501 ttgttaaggc ttgcctgggt ggtttgatca ttcaccacca ccgctatggc ccccagggca
     7561 ttaatggcca gtacggcgat gggcacatgg atatggttgg gacataccag cagcacccga
     7621 tcccactggc ctacacctga ggccgccatc tgctgggcga ggctgcacac caggccaaaa
     7681 gccgcttggt aactcacctg ctggaagtcg ttttttaaat agggggccga gccgaattgc
     7741 tcggccgctt ggctaaaacg ggtaaacagc gtgcgcatac aaccccctag gcgtcggcag
     7801 tgtgttgggc ggctttggcg cagtaaggct ggcgcaggtc ttcggcccca tacaggccaa
     7861 ttttgcacag gtagttttca aagccctggc tccacttatc cacctgctgc tggcgctgat
     7921 ctgtggcggc ggcttccatg gctaggcggg tggggttttc aaggcctatg tctttgtaaa
     7981 tgcgcgggtt gtacaggctg ttaaacgaca ccttggcgta ggtttttacc agttctttaa
     8041 tctgggggat ttcatgctca aggccgcgct cgcgcatttc ccataaattg ccttccacca
     8101 tggcgcggtc ccaagctatg tggcgcacct catctaacag gtgaaagtag tgaatttgcc
     8161 gcaccgtatc gtctatatcg tcccgttcac gggccaagcg attcatgtag cctaaaaaca
     8221 tttccagcac ataggtgcgg gcaaaaaata ataggtcttc gccgtagggc gagagttttg
     8281 ggtctgccat ggcgatgctg cggttgggca taacgccgcc gtggtagcgc acgcaatacc
     8341 cggccagcat aaaggtgtgg gaattttctt cgtggataaa atgctgggcg tattcactgg
     8401 atattgcatc ttcgccgaca aatttattgc ggtagatttt tttctccata tgggaaatta
     8461 aatgctgctc gccaaaaata ttggcggaga aaaaatttac cgtttctaac agcgataatt
     8521 tccacttctg ttcatcggtg agatcgccgt aaatcgggtg gtggtagagg cttaaaaatt
     8581 cttccggaaa ttgcagggca tccgggttaa gtttgctttg gtaatcgaca cggttaaacg
     8641 tatcaaagct gttttttaag gagctgctcg ttaatttttg ggcaacagcc atcacatcgg
     8701 gtttaatgaa cataaaacac ctcaagagcc taacgcgttt aatggtgtgc gcaaaaacca
     8761 gtggcctgct gcgcagaagt cggtgggatg atagggggtt aaggggaagc ggttaagggc
     8821 tggcctgtca gttattcgct taatgagtgt cagcaagtgg gcctgctaaa tgccacctag
     8881 cgcgcgtttt agcctgcccg gcgcgcgcgg ccgggccgca accgttatgc ttagccgact
     8941 aggtgcagct ccaattacct gccactgagc ggccaaatga gcggagccga gattgtatga
     9001 gccctgccag aatgcaggcc aaagcggtcg cttatgctct agcggcctca ataggtgcaa
     9061 aatttacagc gccaccccca actatataac agggcatgct gtgatgagcc catggaggag
     9121 aaaaagtgtt attactgcgg gaattatcgg caattgtttt gttaatggtg tttttaaccc
     9181 ttagcgccag ctgtattttt atagagttta gtttacgcaa cgccattatc gccgcctgtt
     9241 gtttactggc ggcgttttta atctggccga gtaaacgcag ggggcagcgg caggatgatt
     9301 tttggttatt tgatttggca gaattgttaa ttgaagggcc ggtattatta atttcggcgt
     9361 tggctaaatt gttcgattaa tggggcttgt ggggcgttta ggctgtggaa ttaattgagt
     9421 tcgcggttta ataaaacggc acccccctgt gccgcctcta tcgccaagca cagcagaaac
     9481 ttttactgag taaaatttct ccgataatgg ttaggtttaa gcacagtgtt cgatactatc
     9541 gcaaccaggc aaaaatccaa caatttagtt tgtcctttaa acgagccagc aggcactgac
     9601 taactggatt gatggggcat gttgcagccc agccaatggg gtggggctta cttaaaaagg
     9661 cggtcaaagg cgaacgaatc gccgatgctg acattgccag tatctgccgg taacggagtt
     9721 gggcatggct tggcaccaac actcttggct attcctgcag cctgagggct aaaataaaaa
     9781 tgcgttacta aattctccat gtcaaacctt gaaaacacgg cggcatcttt ataccccttt
     9841 gcgatagcaa tcgtgattgg gtccccaagt ctaaaactta attgccgtgc tttttcttgg
     9901 tctttctctt caaggtgatg ccacataaaa atacccctat atattattag agaataattg
     9961 atatttatat taagtcttga ggaatgcctt tattttttaa tatggttgat attttacttt
    10021 gtatttcaga aatatcagca ggattgatac taacagcttc ggcaacaagt atggccacgc
    10081 ctagctttga aagtgaatct atttcatttt tataaattgc agcctccaca gcctgaggag
    10141 ttccaggcct catacgtacc atctgagaaa gcaatggaga aatgttattt cttgctttta
    10201 cccaatcttc ctttgtaatt cttttggcag atgcagcaac ccaattgcct gtagacttta
    10261 ctggatctaa aaaatcaaaa aatcccattt tctctcctca ttgcttaaat gcatcataac
    10321 gacagtactc tctagacttc ttcttcattt ctctaattct gtggcacgcc taaacctaac
    10381 attacattaa taaagatttt gcaattggcg gcgggccgct tggcctatat cgccgccgtc
    10441 ttgggcagca aattcaaagt agcggcggg
//
LOCUS       DAOQGS010000074        10459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_2975_length_10459_cov_4.621492, whole genome shotgun sequence.
ACCESSION   DAOQGS010000074 DAOQGS010000000
VERSION     DAOQGS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_10459_cov_4.621492"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1032)
                     /locus_tag="PKZ52_04330"
     CDS             complement(85..1032)
                     /locus_tag="PKZ52_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNG59029.1"
                     /translation="MSKIVVAALYKFVALPDFAALREPLLEFCIRQDLKGTLLLAHEG
                     INGTVAGSRGAIDALIAYLRQDPRLADIEYKESFEDQQAFYRMKVKLKKEIVTMGIEN
                     IDPNKDVGTYVAPKDWNALIADPDVVLVDTRNYYEYAIGTFKNAINPNTTTFREFPNY
                     VASHLNPQQHKKVAMFCTGGIRCEKSTAYLKQQGFSEVYHLKGGILKYLEEVPADQSL
                     WEGECFVFDNRVAVNHNLEKGQYDQCHGCRHPITAADKESEFYLEGVACPRCYHQQTP
                     DQRQRFAERQKQIQLAKARNQTHVGAPPPARQKISAPKS"
     gene            1252..1728
                     /locus_tag="PKZ52_04335"
     CDS             1252..1728
                     /locus_tag="PKZ52_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNG59030.1"
                     /translation="MCSLYEWRLSEVYMLTISLLVGSVTGNAQAAAEAMAARLTQRGF
                     SVVLIRDGRLPEEAGPWLICTSTTGSGDIPDTIWPFYQRLTKGLYLPEQSFAVLALGD
                     SAYAHFAQAGRDIYSALIDCAAKPLADIYCIDAIYETNPSACALEWLETWIDKLHA"
     gene            1721..2242
                     /gene="fldB"
                     /locus_tag="PKZ52_04340"
     CDS             1721..2242
                     /gene="fldB"
                     /locus_tag="PKZ52_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HNG59031.1"
                     /translation="MRKKIALFYGSSTCYTQMAGEKIAQCLGAAQVDVFNVADEPLAS
                     CQFYEFLIFGIPTWDYGELQEDWEEIWPELDALNFSGKQVALYGLGDQQGYPEWFLDA
                     MGYLHAKLIQRGATCVGFWPVAGYEFEVSQALTPDGNYFVGLALDEETQFELSEARIS
                     QWCTELKTLWNLA"
     gene            2261..>3052
                     /gene="mnmD"
                     /locus_tag="PKZ52_04345"
     CDS             2261..>3052
                     /gene="mnmD"
                     /locus_tag="PKZ52_04345"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNG59032.1"
                     /translation="MDADTPCCAAIVWDANGEPISQQFADVYFAKEQGLDESRYVFIG
                     QNNLPQRFAALAEDAVFIIGETGFGTGLNFLATLEVWRQYAPPSAQLHFISAELYPLK
                     HADLQRALALWPQLSECAEELLSTYPPVFSDECNKGYFRLSLAGNIHLTLLFADATQA
                     FNQWVPQYLGKQLMPAQHNLSTGGKSWAVDAWFLDGFAPSKNPAMWQVDLFRALANLS
                     KPGTTLATFTAAAIVKKGLQQAGFAWQKQRGFGRKRDMLTAVCLPF"
     assembly_gap    3053..3070
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3071..4280
                     /gene="mnmC"
                     /locus_tag="PKZ52_04350"
     CDS             <3071..4280
                     /gene="mnmC"
                     /locus_tag="PKZ52_04350"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNG59033.1"
                     /translation="RHNSGHHINWHLTRRHQETGKRVLVIGGGMAGCHTAFALAQRGF
                     SVTLTDAQAIASGGSGNAQGIVYATLSHTPGPFADFNLAAFLFACNYYRHTQLFARAG
                     AQTGVLDLFNDASELADIAGRFRGNAQWVRPVDSCEASALANVPILSSALFYPRAGWL
                     NPPQLCQQLTAHNNIRVLEHSAVSALDYAHNQWHTSVGAFDQVIIAAAQDCLEFSSAQ
                     AIKIKAIRGQVTAVNKVAELNCVICGDGYIAPAQGSIMHTGASFNPKSASLAILPEDT
                     ALNLANAARLSPVFEQLHPVSDRAGLRCVTPDYLPIVGPLPKPEFLQQFSAYNSNRWA
                     YVDTPAAFYPQLFIVTGLGSRGLTYSPIAAQIIACLIAGEPLPISPELFKFIHPARFW
                     VRDLIRGVLP"
     gene            complement(4290..5705)
                     /gene="hemN"
                     /locus_tag="PKZ52_04355"
     CDS             complement(4290..5705)
                     /gene="hemN"
                     /locus_tag="PKZ52_04355"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNG59034.1"
                     /translation="MHTIQTMHTPLTWDESLIKRFDLSGPRYTSYPTAPHFSPAFNLE
                     DWRAAALRSDLSQAPLSIYCHIPFCETVCYYCGCNKIITANKKRALPYLAALKTEITL
                     QAQYFNNQRPVVQFHFGGGTPTYLSDDQLTDLMAHFRKHFNFATEDQLEASIEIHPQT
                     VTPERLAALKKLGLNRISLGIQDFNAEVQKAVNRFNSAEEVAHLCQAARRLDFKSISM
                     DLIYGLPKQTPASFSATVDSIIALKPDRLSLFSYAHMPHLFKVQQQIIASDLPSSRDK
                     LSMLHLSIDKLQAAGYRYIGMDHFALPDDELAQAQDAGQLHRNFQGYSTRGGCDLLAF
                     GVSAINQVNDTYAQNFKDITAYQNALEQGQLPLHKGLRLSRDDIIRKAVINQLICHFH
                     LQYQAIEARFGLVFKDYFADALAELAPLAEANLCRLSDTGIHITDPGRLLIRRVCMAF
                     DAYLADQNQEPEQAKRYSKII"
     gene            5835..6323
                     /locus_tag="PKZ52_04360"
     CDS             5835..6323
                     /locus_tag="PKZ52_04360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNG59035.1"
                     /translation="MSDPLAQSLKLGMRRLASSVCIVTAANEQGEAQAMTVSSVTSVS
                     AEPASLLVCVNRDSRFAPLLQAGLDLVVNVLHSRHQGLAQLCANPAQFDQRLNDKAWH
                     WQGALPYLADAEAVFFTRVDTLLPYGTHFVVVAQVIQVNVSGATLSPLLYADGSYRSL
                     GR"
     gene            6325..6933
                     /gene="rrtA"
                     /locus_tag="PKZ52_04365"
     CDS             6325..6933
                     /gene="rrtA"
                     /locus_tag="PKZ52_04365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HNG59036.1"
                     /translation="MLRFIPWIFIALILASGLLPEAICEQLDFNPERIKQGEYWRLLT
                     GQWVHFGFNHSLMNAAGLAIVQYSFLDELPLRGWLWVQGAVLSAVAFGLLVFNPEIGV
                     YRGYSGAFIGVLCFSLLHFWQRAPWVALIFFGGLSIKILLEHLPNYDIHYLQSLIGVA
                     VAVDAHLYGYIMGILAQSIYWVWQLLHRKSVAADARENQGEA"
     gene            complement(7254..10325)
                     /locus_tag="PKZ52_04370"
     CDS             complement(7254..10325)
                     /locus_tag="PKZ52_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG59037.1"
                     /translation="MTRLHFRFMDARPALGWQLIRTGCAGLLLISILLVLCGQARANQ
                     APRDNMQFDHPFSQDAEDALNALGIIRFITQDQAGYMWFVFRQGLARYDANRLKPYLV
                     VDEHNTTPIIPSSFTNLVEDKNGVLWIGGEGGLAFYTRQKDRFELVTAANTRWKLPDT
                     KITALAVDKNNRLFIGGDAGLAVVSADRNHVDFYSSAVCGLGNSLIRSLYVARDQTLW
                     IGTTNSGLHAFNADSGNFKHWHIDLGAINSPADDDILRIVDDYDGTIWAATLSMGLAK
                     MNPDQNSFSLYTRDNQAGSLKSNSIRDLLVDTDNTLWVASDHGGLARFEPSTGQFITQ
                     EYSPFNPAGLTSNQVRSLFQDRDGNYWVGTFPASINYYDQRKSRFRVFTQHQNGANYL
                     LSQGILAVHEDKTGTMWIGSEGGLTQLNPADHSVKNYTANPADPNALLFNAITSITED
                     AQGFLWASSWSGGLHKFDRASGNFKRYTPNKNLPNSLPSLYAWQLAINKQGDLWVSFS
                     DRGALARYRPASDDFEHFPHQPQEENSPSADEIWAVLPDKSGNLWLGTRLGLDYFNLT
                     SKQFSHFTLGAGRLNCATVLALMEASDGRIWLGTDGGGINIYDPKNNNFVYLTRQNGL
                     PSGSVTSLLQDPRGYVWAGTLNGLARIDTHTLAIDTLRKSDGLAGDNISRNALKLDSK
                     GYIWVGSTTGLTQFNPDTLKAPLPAPQVVLTQLTINNQVQIPSAAHSVLTQSLEFTQS
                     LQLAYSSKILQVEFSALSYNTPQRNLFAYKLEGFDQEWNPSGNQHQATYTNLDPGTYV
                     LRVKAANSDGVWNDQGRALQIIINPPPWRSSWAYLIYTLALAIIIVAARQLAKQRKAL
                     EQYKALSTVDTLTGIYNRAGIAAIVEGLFINSEIKQGVSLIMLDVDHFKRINDRRGHE
                     AGDRILQELVALVNRNIRSGDYFARWSGEEFILLCPSTSLAHAKKLAEKLRLACANYL
                     FEANSAPLAVTLSIGIASCKPTDDFSQLLKRADTALYRAKNSGRNCIEVED"
BASE COUNT         2398 a         2429 c         2759 g         2855 t
ORIGIN      
        1 ttgccaaatt cgcctagcta gtgcttcgcc gcctacattt ttcagaggtt ccttagggcg
       61 atttagccaa tcggtttatt caatttaact tttcggggcc gatatttttt gccgcgcagg
      121 gggaggcgcg cccacatggg tttggttgcg cgctttggcc aattgtattt gtttttgccg
      181 ctcggcaaag cgctggcgtt gatcgggtgt ttgttggtgg taacagcgcg ggcaggcgac
      241 gccttctaaa taaaattcgg attctttatc ggcagcggtg atggggtgcc tgcagccgtg
      301 gcactgatcg tattggcctt tttctaagtt gtgattaacc gctacgcgat tatcaaacac
      361 aaaacattca ccttcccata ggctttgatc ggcgggaact tcttcaaggt attttaaaat
      421 gccgccttta aggtgataca cctcagaaaa accctgctgt tttaaatatg cagtggattt
      481 ttcgcagcgg atgccgccgg tgcaaaacat cgctactttt ttgtgttgct gggggtttaa
      541 atggctggcg acgtaattgg ggaattcgcg aaacgtggtg gtgttagggt tgatagcatt
      601 tttaaaagtg cctatggcat attcataata attgcgcgta tctaccaata cgacatctgg
      661 gtcagcaatt aaagcgttcc aatctttggg cgcaacataa gtgccgacgt ctttattggg
      721 gtcgatattt tcaatcccca tggtgacaat ttcttttttg agttttacct tcatgcggta
      781 aaacgcctgt tgatcttcaa acgattcttt atattcaata tcggctaagc gcgggtcttg
      841 gcgcaaataa gcgatcaacg catcgatggc gccgcggcta ccggctacgg tgccattaat
      901 accctcatgg gccagtagta aagtgccctt taaatcctgg cggatacaaa actctaatag
      961 gggttcccgc agcgcggcaa aatcgggcag cgcaacaaat ttatacaggg cggcaacaac
     1021 tattttgctc ataactcata cctagtgtgt tagggcgcgc attgtaatca gcctattggc
     1081 gaaaatctag ccaataaatt cccgcttaca ccgctgtttt ggctattggc gaatactcgc
     1141 ttaagccctg gtattttcct gtatggtcgc ctgggcaggc actgcaaaaa gccgtaccat
     1201 tgcgggcttt tttatcaact taggtggcca tgctcaagag gcgcaagcat agtgtgcagc
     1261 ctgtatgaat ggcgcctgtc ggaggtgtat atgctgacga ttagcctatt ggtgggcagc
     1321 gtgaccggta acgcccaggc ggcggccgag gcgatggcgg cgcgtttaac ccagcggggt
     1381 tttagcgttg ttttgattag agatgggcgc ctgccggaag aggctggccc ctggcttatt
     1441 tgcacatcaa ctaccggctc gggggatatt cccgatacga tttggccgtt ttaccaacgc
     1501 ttaactaagg gcttgtattt acccgagcaa tccttcgccg ttctggccct gggcgatagt
     1561 gcttatgcac attttgccca agcaggccgc gatatctaca gcgcattaat cgactgcgcg
     1621 gccaagcccc tggccgatat ttattgtatt gatgccattt atgagaccaa ccccagtgcc
     1681 tgtgcacttg aatggctcga gacctggatc gataaactcc atgcgtaaaa aaattgcttt
     1741 attttatggt tcgtccacct gctacacaca aatggccggg gaaaaaattg cccagtgttt
     1801 aggcgctgcg caggtggatg tgtttaatgt ggctgacgag cccttggcga gttgtcaatt
     1861 ttacgaattt ttaatattcg gcattccaac ttgggattac ggcgaattgc aagaggactg
     1921 ggaagagatt tggccggagt tggatgctct taatttcagc ggtaaacaag tggccttata
     1981 cggtttgggg gatcagcagg ggtatcctga atggtttttg gacgctatgg gttatttgca
     2041 cgcaaaatta attcagcgcg gcgcaacctg cgtggggttt tggccggtag ctgggtacga
     2101 gtttgaagtc agtcaggccc tcacgccgga cggcaactat tttgtcggcc ttgctctgga
     2161 tgaagaaacc caatttgagc tgagtgaagc gcggattagc cagtggtgca cagaattaaa
     2221 aaccctctgg aatttagcgt gaaatattgg ggcactaggc gtggatgctg atacaccttg
     2281 ctgtgccgct attgtgtggg acgccaatgg cgagccaatt tcccaacaat ttgccgatgt
     2341 ctattttgcc aaagagcagg ggcttgatga gagccgctat gtatttattg ggcagaataa
     2401 tttaccccag cgttttgccg ctttggcaga agatgcggta tttattattg gcgaaacggg
     2461 ttttggcact ggcttaaatt ttctagccac tttagaagta tggcgccagt atgcgccgcc
     2521 atcggcgcag ctacatttta tatcggccga gctgtatcca cttaagcacg cggatctgca
     2581 gcgcgcccta gcactttggc ctcaattgag tgagtgcgcc gaagaattgc tcagcaccta
     2641 tccgccggtg tttagcgatg aatgcaacaa gggttatttt cgcttaagtt tagcgggcaa
     2701 tattcattta acgctgttat ttgccgatgc aactcaggca tttaatcaat gggtgccgca
     2761 gtatctcggc aagcaattaa tgccagccca acataattta tccaccggcg gtaaatcctg
     2821 ggccgtggat gcttggtttt tagatggttt tgcaccgtcg aaaaatcctg ccatgtggca
     2881 agtagattta tttcgagccc tcgcgaatct gagtaaaccg ggtactacac tggccacctt
     2941 tactgcggcg gccatagtta aaaaaggcct gcaacaagca ggttttgctt ggcaaaaaca
     3001 gcgcggcttt ggccgcaagc gcgatatgtt aacggcggtt tgccttccgt ttnnnnnnnn
     3061 nnnnnnnnnn ccgccacaat agcggccatc atattaattg gcatctaacc cgcaggcatc
     3121 aagagacggg aaaacgtgtt ttagttattg gcggcggtat ggccggttgc cataccgcgt
     3181 ttgcgctggc acagcgcggg tttagcgtta cgcttaccga tgcacaagcg attgccagtg
     3241 gcggctcggg taatgcccaa ggcatagtgt acgcaaccct gtcccacacc ccagggccat
     3301 tcgccgattt taatttggcg gcgtttttat ttgcctgtaa ttattatcgt cacacacaat
     3361 tatttgcccg tgcaggggcg caaaccgggg tgttggattt atttaatgac gccagcgaat
     3421 tggccgacat cgctgggcga tttcggggta atgcgcagtg ggtaaggcct gttgattctt
     3481 gcgaggcaag tgctcttgcc aatgtgccga ttttatcttc ggcacttttt tacccgcgcg
     3541 caggctggct aaatccgccg cagttgtgcc agcaattaac cgcccataat aatattcgcg
     3601 tgcttgaaca ctcggctgtt tctgcccttg attatgcgca taaccaatgg catacctctg
     3661 tgggtgcatt tgatcaggtg attattgctg ccgcccaaga ttgcctggaa tttagcagcg
     3721 cacaggccat aaaaattaaa gccattcgcg gccaggttac ggctgtgaat aaagtggccg
     3781 agttaaactg tgttatctgc ggcgatggct atattgcgcc agcgcagggc agtatcatgc
     3841 acacaggcgc aagttttaac cctaaatccg caagcttagc cattttacct gaggatacgg
     3901 cccttaattt ggccaatgcg gcgcgtttat cgccggtgtt tgagcagtta caccccgtca
     3961 gcgatagagc cggtttgcgc tgcgtaacgc cggattattt acctattgtg gggccgttgc
     4021 ccaagcctga atttttgcag cagtttagcg cttataacag caaccgctgg gcctatgtag
     4081 acaccccagc ggcgttttat ccgcagttat ttatcgttac aggtttaggc tcgcggggcc
     4141 taacctatag ccctatagcg gcacagatca tcgcctgttt aatcgcaggt gagcccctgc
     4201 ccattagtcc agaattattt aaatttattc acccggcgcg cttttgggtg cgcgatttaa
     4261 ttcgcggcgt attaccctaa aatcccatac taaatgattt tggaatagcg ttttgcctgc
     4321 tcgggttctt ggttttgatc agctaaatag gcatcaaaag ccatacagac gcggcgaatt
     4381 aataagcggc ctgggtcggt aatatggatg ccagtatcgc tgagcctgca taagttggcc
     4441 tctgccaagg gggctaattc ggcgagcgcg tcggcaaaat agtctttaaa taccaggcca
     4501 aaacgcgctt ctatggcttg gtattgcagg tgaaaatggc agattaactg gttgataacg
     4561 gctttgcgga taatatcatc ccggctgagg cgcaggcctt tatgaagtgg taattgccct
     4621 tgttctaagg cattttggta ggcggttata tctttaaaat tttgtgcata ggtgtcattt
     4681 acctgattga tggccgatac gccaaacgcc aacaaatcgc agccgccgcg ggtggagtag
     4741 ccctggaaat tgcggtgtaa ttggcctgca tcctgggctt gggctaattc atcatcgggc
     4801 agggcaaaat ggtccatacc tatatagcga taacccgctg cctgtagttt gtcgatactt
     4861 aaatgcagca tgctgagttt atcgcggctt gagggtaagt ctgaggcgat tatttgctgt
     4921 tgcaccttaa atagatgcgg catatgggcg tagctaaaca gtgataagcg gtccggtttg
     4981 agggcaataa tgctatcgac tgtggcgcta aaactcgcag gcgtctgttt gggcaggcca
     5041 taaataagat ccatgctgat ggatttaaaa tcgagcctgc gcgcggcctg acatagatgc
     5101 gccacctctt cggcactatt aaagcgattc accgcctttt gtacctcggc attaaaatct
     5161 tgaataccca aactgatgcg atttaaaccg agttttttaa gcgcggccaa gcgctcgggg
     5221 gtcactgttt gcgggtgtat ttcaatgctg gcttcaagtt gatcttctgt tgcaaaatta
     5281 aagtgtttgc gaaaatgcgc cattaaatcg gttaattggt catcacttaa ataggtgggt
     5341 gtgccgccgc cgaaatgaaa ttgcactacg ggcctttggt tgttgaaata ctgtgcttgc
     5401 agggtaattt ctgtttttag cgccgctaaa tagggcaggg cgcgtttttt attggcggtg
     5461 attattttat tgcagccgca gtaataacac acagtttcgc aaaatggtat gtggcaataa
     5521 atagacaggg gcgcttggct tagatcgctg cgcagtgccg ccgcgcgcca atcttccagg
     5581 ttaaaagccg ggctaaagtg aggcgcagta gggtaagacg tgtagcgggg gccgctcaaa
     5641 tcaaaacgct tgattaggga ttcatcccag gtgagtggcg tgtgcattgt ttggattgtg
     5701 tgcatatggc ccctgcgtgc aaaaacagca gcataaacat ttgccggcac gggcccttga
     5761 tgccggtcaa ctggccgtag gcagcatagg cctggcgcgg tattatgacg gcctttttta
     5821 gttggatcct gtctatgagt gaccccttgg cccaaagctt aaaattggga atgcgccgtt
     5881 tggcttccag cgtgtgcatt gttacagccg ccaatgagca gggtgaagcc caggcgatga
     5941 cggtcagttc agtgacttct gtgtcggcgg agcctgcttc tttattggtg tgcgtcaatc
     6001 gcgacagccg ctttgcacca ttgctacaag cgggtttaga tttggtggtc aatgttctgc
     6061 atagtcgcca tcagggcctt gcgcaactgt gcgctaaccc cgcccaattt gatcagcgcc
     6121 taaatgataa agcctggcat tggcaggggg ctctacccta tttagcggat gccgaagcgg
     6181 tattttttac ccgtgtggat accctattgc cctacggcac ccattttgtg gtggtggccc
     6241 aagtgatcca agtgaatgtc agtggcgcca cgctttcgcc gctgttgtac gccgatggca
     6301 gttaccgcag tttaggccgt taatatgctg cgttttattc cctggatttt tatcgccctc
     6361 atactggcta gtggcctttt acctgaagct atatgtgagc agttggattt taatcctgag
     6421 cgaattaaac agggtgaata ctggcggtta ttaaccggcc agtgggtgca ttttggattt
     6481 aaccacagcc ttatgaatgc ggcgggcctg gctattgtgc agtacagctt tttagatgaa
     6541 ttgcctttgc gcggttggct ttgggtgcaa ggcgcggttt tatcggcggt ggcctttggg
     6601 ttgttggtat ttaatcccga aattggtgtg taccgcggtt actccggggc atttatcggc
     6661 gtgttgtgtt tttccctgct gcatttttgg cagcgcgcgc cttgggtggc attgattttt
     6721 tttggcggct tgagcattaa aatcctgtta gagcatttac ccaattacga cattcattat
     6781 ttgcaatcgc ttattggcgt tgcagtggcg gtagatgccc atttatacgg ctatataatg
     6841 ggcattttgg ctcagtcgat ctactgggtg tggcagttat tacacagaaa gtctgtggcg
     6901 gcggatgcca gagagaatca aggcgaagca taatttgctc gcaactattc gacacttatt
     6961 gccagtatct ttttttgtaa ctggacgtga agctcctgcc gaatagaaac caccaggcgt
     7021 gtgcgtgagt gctgtcggcc ctcagtactc ccccgcagcg atgaattcag gttatttcag
     7081 acgcgccgta aatacatccg tgtaggctcg tcaaaaaggt ccctcttttt gacggtctga
     7141 aaccgtgtgt actcattgct tccgtgcagc gagtaagagg gtaattaagc tatacctttc
     7201 aataaggaca ttttcagctt tcctcattga ttattgccaa cccggtagta ggcttagtct
     7261 tccacctcaa tacaattgcg cccagaattt ttagcgcggt agagggctgt atcggcccgt
     7321 ttgagcagtt ggctaaaatc atctgtaggt ttgcagctgg caataccgat gcttagcgtg
     7381 acggcaaggg gcgcactatt ggcttcaaat aaataattgg cgcaggccag gcgcagtttt
     7441 tccgccagtt tttttgcatg ggcaagactc gtactggggc ataataaaat aaattcttcc
     7501 ccgctccagc gcgcaaaata atcgccgctg cggatattgc ggttcactaa agctacgagt
     7561 tcctgcaaaa ttctatcgcc cgcctcgtgg ccgcgccggt cgttgatgcg tttaaaatga
     7621 tctacatcca gcataataag gcttacgccc tgttttattt cgctgttgat aaacaagcct
     7681 tctactattg cagcaatgcc cgcgcggtta taaataccgg tgagcgtatc caccgtagat
     7741 agggctttat attgctctag ggctttgcgc tgttttgcca attgccgtgc ggccacaata
     7801 atgatggcaa gtgccagggt ataaattaaa taagcccagc tgctgcgcca gggtggcggg
     7861 ttaataataa tctgcagcgc gcggccttga tcattccaaa cgccatcgct gttggcggct
     7921 tttacccgta atacataggt gcctgggtct aggttggtgt aggtggcttg atgttggttg
     7981 ccacttggat tccattcttg atcgaagccc tctaatttat aggcgaataa attgcgctgg
     8041 ggtgtgttgt aacttaacgc ggagaattca acctgcagta ttttagatga gtatgccagc
     8101 tgtaatgatt gggtgaattc aagggactga gttaatacag aatgagccgc gctgggaatt
     8161 tgcacctgat tgttaatagt caattgtgtg agtaccactt ggggcgcagg caagggggct
     8221 ttgagtgtat cgggattaaa ctgcgttaag ccggtagtgg agcccaccca aatataacct
     8281 ttactatcga gttttaatgc gttgcggctg atgttatcgc ccgccaagcc gtcgctttta
     8341 cgcagtgtat cgattgccag ggtgtgggtg tcgatgcggg ccaagccatt tagggtgccc
     8401 gcccacacat aaccgcgcgg atcttgcaaa aggctggtaa cactgcctga aggcaaacca
     8461 ttttggcgtg tgaggtacac aaaattattg ttttttgggt cgtaaatatt aatgccgccg
     8521 ccgtcggttc ccagccaaat acgcccatca ctcgcctcca tcagggctaa tacagtggcg
     8581 caatttagcc tgcctgcacc tagggtaaaa tggctaaatt gtttgctggt caaattaaaa
     8641 taatccaaac ctaaacgggt ccccaaccat aaattaccgc ttttatcggg caatactgcc
     8701 catatctcat cggcgctggg ggaattttct tcctggggct gatgggggaa gtgttcaaaa
     8761 tcatcactgg ccgggcgata gcgtgccagg gcgccgcgat cgctaaaact aacccataaa
     8821 tcaccctgct tattaatggc cagctgccag gcatagaggc tgggcaaact attaggcagg
     8881 tttttattgg gtgtgtagcg cttaaaatta ccgcttgcgc gatcaaattt atgcagcccg
     8941 ccggaccaac tgctcgccca caaaaagccc tgggcgtctt cggtaataga tgtaatggca
     9001 ttaaataaca aggcattggg gtcggctggg ttggcggtgt aatttttaac gctgtgatca
     9061 gcggggttta attgcgtaag gccgccttca ctgcctatcc acattgtgcc ggttttatct
     9121 tcatgcacgg ctaaaatacc ctggctgagt aaatagttgg cgccgttttg atgctgggta
     9181 aatactcgaa agcggctttt gcgctggtcg taataattaa tgctggcggg aaaggtgccc
     9241 acccaataat taccatcgcg gtcttgaaat aaactgcgca cttgatttga ggttaggccc
     9301 gccgggttaa acgggctgta ttcctgagtg ataaattgtc ccgtgctggg ctcaaaccgc
     9361 gccagcccac cgtgatcgct ggccacccag agtgtattgt cggtatcgac taataaatcg
     9421 cgaatggaat tactttttaa actgccggcc tgattatccc gcgtatacag gctaaaacta
     9481 ttttgatctg gattcatttt ggccaagccc atggaaaggg tggcggccca aatagtcccg
     9541 tcgtaatcgt caacgatgcg caatatatcg tcatcggcag gggagttaat tgcgcctaaa
     9601 tctatatgcc agtgtttaaa attgccgctg tcagcgttaa acgcatgcag gccgctgttt
     9661 gtggtgccga tccatagggt ttgatcgcga gccacataca aggagcgaat aaggctgttg
     9721 cctaacccac acacagcgct ggaataaaaa tccacatggt tacgatcggc cgaaaccact
     9781 gctaaacccg cgtcaccacc gataaataaa cgattatttt tatccaccgc cagggcggta
     9841 attttagtgt cgggcagctt ccagcgggta ttagcggcag tgaccagttc aaagcgatct
     9901 ttttgccggg tataaaaggc taagccacct tcgccaccaa tccacaacac gccattttta
     9961 tcctccacta aattggtaaa ggagctggga ataataggcg ttgtgttgtg ctcatccact
    10021 actaaatagg gttttagccg gttggcatcg tagcgggcta ggccctggcg gaacacaaac
    10081 cacatgtatc ccgcttgatc ctgggtaata aagcgaataa tgcccaatgc attgagggca
    10141 tcctctgcat cttggctgaa ggggtggtca aactgcatgt tgtcgcgggg ggcttggtta
    10201 gcgcgggcct gcccgcataa caccagcaaa atcgagatta ataaaaggcc tgcacagccg
    10261 gtgcgtatca attgccaccc cagagcgggg cgcgcatcca taaagcgaaa gtgaagcctt
    10321 gtcatgacct atgtctaaga ccttataggg gcgtctgccc atggcgatta ttgaaagcgg
    10381 cccattgctc agttaaagcg gcgcctttaa cagcttgttg aaatactacc ttgctagctc
    10441 tggcggcgtt aaatttggc
//
LOCUS       DAOQGS010000075        10273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3043_length_10273_cov_5.491290, whole genome shotgun sequence.
ACCESSION   DAOQGS010000075 DAOQGS010000000
VERSION     DAOQGS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3043_length_10273_cov_5.491290"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKZ52_04375"
     CDS             <1..287
                     /locus_tag="PKZ52_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59038.1"
                     /translation="GESVEVEVLNLLWSIVEPAKQTPDLAVHYAAIRISKLPIFTLRA
                     YHSVGGQRVYWHPLQQTFSSFQTPDTVIAQAHAAYPAEDIIKRWVRDNLK"
     gene            291..1565
                     /gene="mobH"
                     /locus_tag="PKZ52_04380"
     CDS             291..1565
                     /gene="mobH"
                     /locus_tag="PKZ52_04380"
                     /inference="COORDINATES: protein motif:HMM:NF041494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HNG59039.1"
                     /translation="MLKQTNELNLSALPILGADQLLKRVNIDSRLNDWRTLMAFHDTA
                     FNDLIQSPFFRYVEAIQLCPASEADHHAGPGGLLAHTYDVISLALKRRRGLQLPLGGT
                     VEQIRDQRHLWTYAIFAAGLLHDIGKLMGSIRICLRFRNNTFMLWTPQDKPLKEFEQA
                     VSYSVEFVKTPYSYHTQIALTLFDFLPQTGRAWLARDQTIMMQLCAYLRGDRFESGVI
                     GEVLEQADMSSTAAAQLRPAQLRFSNRPSTIERLIHFVRQWIAEDVIRINKNGGMAWC
                     TGEYIYIVCRALADKIIKHCDDANITDLPRDPVRLYDIFQDYGFAVPTPDAKAIWNVV
                     ISCPEYTHRFTCLKFKLRRLVVPTRIPAPFVGDIKEVFGEAKKQKPIPTTAVSETFPE
                     SADSIVAECEEADAITLDNAAESNSSQEGGGR"
     gene            1522..2430
                     /locus_tag="PKZ52_04385"
     CDS             1522..2430
                     /locus_tag="PKZ52_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNG59040.1"
                     /translation="MQLNQIHHRKGGGDEPIESAQEIEQETTHSLDDPKLQSDQAAVV
                     DKESESNSIHHESGELLGTLGDDSLEDSEEVFSDTVDAIFPDQEADFAETLPETAALE
                     GEVIFAAVAHDEKHSESASDDDQDAGANQETADVSSVKKLQKKSLDKKFGKYLVSGKP
                     SFEPGVVAIDADQVAYMFIRWLRENILNKSIAMNNTEALVHIMEDCVLTLAPGIFKCF
                     LEVHGIDGEANFKALGRKVRLIRKHIPAPDGLNIHPVYAQGKNKTTKLHGWKFPFNLF
                     FDDPSTIPAANIYIKNEPGFTGSKKS"
     gene            2545..3507
                     /locus_tag="PKZ52_04390"
     CDS             2545..3507
                     /locus_tag="PKZ52_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNG59041.1"
                     /translation="MKFLDLAREYSDLNVFTPDTQLTLLRAARLFVNRTGIEHCEEIG
                     RDHVIQFKAVTLSRARPITYNGYLRYLRLIGDYAVDQNLMDKNVFRYAKTAPTGKKIP
                     KTQSIDSVMAALQHLEAGGYDYEPIWFWQAVIKCLYYTGIRRKQLVNIEVGDIDFKAS
                     SLKLNYEGSKTHREWYIPIHSDLTATLHKLQDELVRVLGRSLRPTDTLFNLAFLSNRY
                     CRDPRRIDGLRPRAITDFFKRIKRNSGICVSAHRFRHSLATELCNPIDGQADILSAQV
                     MLGHTSLQTTRVYVQTSVAQIGHSLNKIPTLNTKLSSEVPPMPI"
     gene            3776..4147
                     /locus_tag="PKZ52_04395"
     CDS             3776..4147
                     /locus_tag="PKZ52_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNG59042.1"
                     /translation="MKIIGIISTKGGVGKTTLTANLGAILADMGQKVLLVDADPQQTL
                     SRFYAIRDQTPFGLTQLFKSSNPAKCISTTVIDGLDIVIGDDKKSDGVIPALLNESLA
                     NIQNLAAALHQVKLRYVVGTD"
     gene            4590..7313
                     /locus_tag="PKZ52_04400"
     CDS             4590..7313
                     /locus_tag="PKZ52_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG59043.1"
                     /translation="MRKILILGMLSAMLAACGGGGSSSSSSSSSSSSSSSSSTSAGKT
                     SSSSSSSSSSSSSSSSSSSSSSGNISSSSSTGGFSGDAARGKTTFASRCARCHKDDNG
                     DGYFGSDEASGDSGIAVIDPDSLGNPASDYPAYDGRAVTQLAAFIQNSMGPVANCNAQ
                     CAQDAAAYIWSLKDGQKGPGAAAVSCSNPGAIHYGKRVLHVLTSYEYHNSLSKLFALP
                     LPNDYSSAGKVGLDTEVARLPNHSIEAINESRLNKYYDNAIEIADWAMATRGALPFAC
                     DDAVQCSTDFINNFAYVAYRRALTSVEQSEITSIFTGAPTLEDGLKWALRTVLMSPNF
                     LYRSELGTKVADLLKNPVVSQGTKYEFQGTPKTFSTLSIQRYNRISVTATSNPGYQWT
                     GKDLIAITVKGEKSPMNNWPVLTIGTGYDNSGVLKRLTIDHTGYRTYYITIDSLPSGP
                     VYFLNDASFANNDYQMPPLQISSISFGVLGQSKPVQDDVAKLTAADQNAYVLDPYEYA
                     SALSFALTGSAPSKNLLDAAVAGELVDVVKREAQVDALIDSALGKAHVERLAGKWFRT
                     DGLSEKTRNDAEFTPAVRQSMMQEIREIYKHVFYNGNFPSMYSGDYTFLDSTLSTFYK
                     LPGGGDAPGSFRMVSTANTQRGGVIASGAYMAYNAHMDYSSPIQRSAHFRQDVLCQSI
                     PLPVNLEDSQEREAAAQLVKSRVEAGDITTTDFYDIQTNIPSSSCATCHNAIINPLFG
                     MDDFDHVGRLRPKVNNKVVQDGLIFVNGVAVPKGKANLDITQVNNGSYLYAYNVVGTL
                     SGAKADAAKASGDGLKFNGAKDLGKVIVQNDLPGISACLIEKSTRYALGYSLDKAFLD
                     PIENGRYGISNTQEAEFACIKNELNTAYARAKSPRDVVKAAVMSDTFRFRK"
     gene            7373..8668
                     /locus_tag="PKZ52_04405"
     CDS             7373..8668
                     /locus_tag="PKZ52_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG59044.1"
                     /translation="MNDSKKLPQPNDTIAEAQRKDVHKSRRKFLDLVAKSGIGSATLK
                     YSSLLGGVMTARYASAADAPKRVVYLYIHSGSPDDWRPSSASAISTNQSSYHYGPASY
                     NVAQICHFRGVDTEVGGHGGARQALGGDYSRNTVDADLGAILGPRSYYSSIYLGSNAT
                     DSGTGAGVIISSAGQPEDDPRRALSRYFGAVPETSKDDTYILSFDAQKRALENIKKKL
                     STDEYNRVQTHVTAIEKIESRIADLANATAPDLNACKPTLPGTYDDTTNAAMVAHAKL
                     QADILVAAFKCDLTRVGVLQIGNHNGAGWTYKGFDGHSAAHSGGASVWNPMMRDKFEV
                     PAYFIKKLMDTSDSTGQPLINSTAFVQVTCFGNGLSHASTNAPFLLATKMPSFKPGFS
                     SKGTASTSRDFHAEVAKGLGVDPKGLKLGGATGGDVGLV"
     gene            8805..10184
                     /locus_tag="PKZ52_04410"
     CDS             8805..10184
                     /locus_tag="PKZ52_04410"
                     /inference="COORDINATES: protein motif:HMM:NF019554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59045.1"
                     /translation="MTQFNFPPGILVCPVCAQQVVADRCGGCNTEFYSLGEIPCLFTT
                     GIQQKNLWQHQMAMMESQGADALEHLDYIMQGYDLSRLTRDRLEQARAAMSHSLTVIL
                     GQLKNAGLQSVRDPVFEQMRVDNPTEYYHHILRDWAWDAVPSRHFKTHVNDANLERVL
                     NVWPNPAPGKMLFLGAGAGRLSWDLHRILKPEFTIAADINPFLLSCAHSLIKARESIT
                     LPELYTYPQIGFPYAKSWLMEPPQDPDGLHRNWYALGADVWNMPLQEALMDSVVTSWL
                     LDVTGGDVKDLIAVVDYLLKPGGYWINTGPLLYSAQLNFDLKYSAEEILEFADMAGFD
                     VEELSVEEAEHMASPLNARYHHEQLFSFSARKRTQLKPLPEPGNTSEWLTPAWLVMHH
                     IPVPKIQFNCQIGHEFIARVLDLVDGKRSIYLIAQLVQPHLPEGVDAKEAVVALFGQI
                     LEGMAGLGE"
BASE COUNT         2614 a         2537 c         2516 g         2606 t
ORIGIN      
        1 ctggcgaatc cgttgaggtt gaagtcctga atctgctgtg gagtattgtc gagcccgcca
       61 aacagactcc cgatttggca gtccactatg ccgccattcg catttctaag ctgccgattt
      121 ttacgcttcg agcctatcac tctgttggag gccagcgggt ttattggcac ccgctgcaac
      181 aaacattctc cagcttccaa acgccggata cggtcattgc ccaggcgcac gcagcatacc
      241 cggctgagga cataattaaa cgatgggtga gagacaacct gaaatagctt atgttgaaac
      301 aaacgaatga gctgaattta tctgccttac ccatattagg tgccgatcaa ttattaaaac
      361 gggttaacat tgattcgcgc ctaaatgact ggcgaaccct gatggcattt catgacacag
      421 cgttcaacga tctgatccaa tctccctttt ttcgctacgt tgaagccatt cagctgtgcc
      481 ccgcaagtga agctgatcat cacgctgggc ctggtgggct gcttgcacac acctacgatg
      541 ttatatcact ggccctaaaa cggcgtcgcg gtctacaact gccactcggc ggcaccgtag
      601 aacaaatccg tgatcaacgg cacctatgga catatgcgat ttttgcagca gggttgttac
      661 acgacatcgg caaattgatg ggctccataa ggatttgttt gcgctttcgt aacaacacat
      721 tcatgttgtg gacgcctcaa gacaagcccc tcaaagaatt tgaacaggct gtctcctaca
      781 gcgttgaatt tgtaaaaacc ccttatagct atcacacaca aattgctttg accctatttg
      841 attttttacc tcaaacaggt agagcctggt tggcccggga tcagaccatt atgatgcagc
      901 tttgtgcgta tttgaggggg gatcgttttg aaagtggtgt gattggtgaa gtccttgaac
      961 aggccgatat gtcgtccact gcagcagcac aattaagacc tgcccagtta cgttttagta
     1021 atcgcccttc aacaattgag cgcctgattc actttgtccg gcagtggata gccgaggatg
     1081 tcatcagaat taataaaaac ggaggtatgg cttggtgtac tggagagtat atttatattg
     1141 tgtgtcgggc gctggccgat aaaatcatta agcattgcga cgatgccaat attaccgatt
     1201 tacctcgcga ccctgtgcgg ctatacgata tttttcaaga ttacgggttt gccgttccca
     1261 ctcctgatgc caaggctatt tggaatgttg ttatatcctg ccctgaatat acccatcgat
     1321 ttacctgcct caaatttaaa cttcgtaggc tagttgtacc cactcgcatt cccgcaccgt
     1381 ttgttggtga tattaaggaa gtatttggtg aagctaaaaa gcaaaagcca attcccacta
     1441 ccgcagtttc agaaacattt ccagaatctg cagattcgat agtagccgaa tgtgaagagg
     1501 ctgacgcgat tacattagat aatgcagctg aatcaaattc atcacaggaa gggggggggc
     1561 gatgagccta ttgaatctgc gcaggaaata gagcaggaaa ctacgcattc acttgatgac
     1621 cctaaattac aaagcgatca ggcagctgtg gtggataaag aatctgagtc aaatagcatt
     1681 catcatgaat ctggggaatt gctgggcaca ctgggtgatg attcacttga ggattctgaa
     1741 gaagtgtttt cggatactgt agacgcaatt tttcctgatc aggaagctga ttttgcagaa
     1801 acgcttcccg aaactgcggc ccttgaaggt gaagtcattt ttgcagcagt ggcgcacgat
     1861 gagaaacatt ccgaatctgc atctgatgat gatcaggacg ctggcgctaa tcaggaaact
     1921 gccgatgtat ccagcgtcaa gaagctgcag aaaaaatcac tggataaaaa attcggcaaa
     1981 tatttagtgt caggcaaacc gtcatttgag ccaggtgtag tggccattga tgctgatcaa
     2041 gtcgcctata tgtttattcg atggctgcgc gaaaacattt taaataaatc catcgcgatg
     2101 aataacactg aggccctggt tcacatcatg gaagactgtg tactgacatt agccccgggg
     2161 atttttaaat gctttttgga ggtacatgga atcgatggcg aggccaactt taaggccctg
     2221 ggccgtaaag tacggttgat tcggaaacac atcccggcac cagacggctt gaacattcac
     2281 cccgtctacg cccaggggaa aaataaaaca accaagctcc atggctggaa attccctttt
     2341 aatctatttt tcgacgatcc gagcactata ccggcggcca atatttatat aaaaaacgag
     2401 ccaggcttca ccgggtctaa aaaatcttaa atggcgcacc tttcaacata agcaattttt
     2461 ggcaataatg acgtcacagc taatagcgaa tagctattaa tttgtggtgg catgatgcca
     2521 aattattatt agaaggtgac aagaatgaaa tttttggatt tagctcgtga atactctgac
     2581 ctgaatgtgt ttacccctga tacccaactg accttactga gagcggcacg actctttgta
     2641 aaccggacag gtatcgaaca ttgcgaggag attggacgag atcacgttat tcagttcaag
     2701 gccgtgacgc tgtcccgtgc ccgaccaata acctacaacg gctacctgcg gtatttacgc
     2761 cttataggtg actatgctgt agatcaaaac ctaatggaca aaaatgtctt tcggtatgcg
     2821 aagacggcgc cgacagggaa aaaaatcccc aagactcagt cgatagactc tgtaatggct
     2881 gcgttacagc accttgaagc tggcggctat gactacgaac ccatatggtt ttggcaggcc
     2941 gttatcaagt gcctttacta cacgggcatt aggcggaaac agctggtcaa tattgaagtg
     3001 ggggacattg attttaaggc cagtagcctg aagcttaact atgaaggttc gaagactcac
     3061 agggagtggt acatacccat ccactcagac ttaactgcca cactccataa actgcaggat
     3121 gagcttgtga gggtgcttgg taggagcctc cgcccaaccg atactctatt taatctggct
     3181 tttttatcca atagatactg ccgggatccg cgcaggattg atgggcttag gccgagagcc
     3241 attacagact tttttaagcg gatcaaacgc aactccggca tatgcgtgtc ggcacaccga
     3301 tttcggcact cgttagccac tgagttgtgc aatccgattg acgggcaggc cgatatttta
     3361 agtgcccagg tgatgctcgg gcatacttcg ttacagacca ccagagtcta cgtccaaacc
     3421 tcagtggccc agattggcca ctcgctcaac aagatcccca cgctgaacac gaagctttcc
     3481 agtgaggttc cgcccatgcc gatataaaca aatatttgac aaaagttcca ttcaagcgca
     3541 agaattgaac ttgcgcttgt tgatgtttaa cggcacatga gacaacagat caataattga
     3601 ccgatttaca gccatttggc gagcacttat agctccggca aacacaacac tcataatgct
     3661 ggggtcgcga gttcgagtct cgccctagcc accatcaggt ggccccgatg tgttccagta
     3721 ctgcggtaga aaaataagtg cagatctaca atcactcaga ttgaggtcta accttatgaa
     3781 aattatagga attatttcta ccaaaggcgg tgtgggcaaa accacactca cggcaaacct
     3841 aggggccatc ttggctgata tgggccagaa ggtacttctg gtagacgccg atccccagca
     3901 aaccctctcc cgtttttacg ccatccgcga ccaaactcct tttgggctga ctcagctatt
     3961 caagtcatct aatcccgcca aatgcatatc gacgaccgtc attgatggcc tcgatattgt
     4021 cattggcgac gataaaaagt cggatggcgt cattcccgct ctgctaaatg agtctttggc
     4081 aaatattcag aacctggctg ccgcacttca ccaggtaaaa cttcggtacg tagtaggtac
     4141 tgattgacac cgagggagcg ccccacaaaa ttctagatgc tgttacctac gcggcagact
     4201 aattgcgctt aagaattcag gtagcctatt ccggtgtgtt ttcgttgtta gcggttcaat
     4261 gaatatcact gcatattcgc aggcatcgaa catgctcacg ccaaaagtgt tatcccacgc
     4321 ctactaccta ctactaactg gttgaactgg ttgtatggaa ggagcggtca actgggcata
     4381 ataaaggcgt taaatcacat atatgacaag tgaaatttat ggcaaatcct ttttacaagc
     4441 atgactataa gtgagtgttg ctgggatcct tgtcacggtt cagaaaccta aactttctta
     4501 atatacgcac tagaaatttt caagtttcag tcgtcagtct cgatatgcgt gagctggtgg
     4561 cggtcgtcaa atgaggagct ttatcgataa tgcgtaagat attaattttg ggaatgttga
     4621 gcgcaatgct cgccgcctgt gggggagggg gtagttcatc ctccagcagt tcatcaagca
     4681 gtagctcgtc gagcagcagc tcaacatcag ccggcaaaac tagcagctct tcttcgagca
     4741 gcagttcatc gagtagcagc agctcctcct cgagcagcag ttcgtcgggc aatatatctt
     4801 catctagctc tactggtggc ttctcagggg atgcagcgcg aggaaagacg acattcgctt
     4861 cccgctgcgc caggtgccac aaagatgaca acggcgatgg ttattttggc tccgatgaag
     4921 cttcaggtga ttcaggtatc gcagttattg accctgatag tctgggcaat cccgcctcgg
     4981 attacccagc ctatgatggc cgtgcagtga ctcaacttgc cgccttcatt caaaactcaa
     5041 tggggccagt ggcgaattgt aatgctcaat gcgcccagga tgctgctgct tatatttggt
     5101 cattaaaaga cggccagaag gggccagggg cggcggcagt ttcctgctct aacccaggcg
     5161 ctattcacta tggcaagcgg gtgttacatg tcttgacgtc ttatgagtat cacaactccc
     5221 tcagcaagct gtttgcctta cccttgccca atgattactc ctcagctggc aaggtaggcc
     5281 tagacactga agtggcgcgg ctgcccaacc attcaatcga agcaatcaac gaaagccgct
     5341 tgaacaagta ctacgacaac gcaattgaaa ttgcggattg ggccatggcc actcgcggcg
     5401 ctttaccatt cgcctgtgac gatgctgtcc aatgttcaac ggatttcatc aacaactttg
     5461 cctatgtggc ttaccgtcgt gctttaacca gtgtggagca gtcggagatc acctccattt
     5521 ttactggtgc ccccacgctt gaagacggtt tgaagtgggc cttacgcacc gtattgatgt
     5581 cgccgaactt cctctatcgc tcggagttgg ggaccaaggt tgccgattta ctgaagaatc
     5641 cggttgtgtc ccaaggcact aagtacgagt tccaaggtac gccaaaaacc ttctccacgt
     5701 tgagcatcca gcgttataac cgcatcagcg ttaccgccac ctccaacccc ggctaccagt
     5761 ggacaggtaa agacctaatt gcgatcacgg ttaaaggtga aaagagcccc atgaacaatt
     5821 ggccggtgct caccatcggt accggttacg acaactcggg cgtgcttaag cgtctgacta
     5881 tcgatcacac cggttacaga acctactaca tcaccattga tagcctgccg agcggccctg
     5941 tttatttcct aaacgacgca agctttgcca acaacgatta ccagatgcca cctctgcaga
     6001 tctcatccat ctcgtttggt gtgctgggac agtcgaaacc cgtgcaggat gatgtcgcta
     6061 agttgacggc cgctgatcaa aacgcttacg tgctcgatcc ctacgaatac gcctctgcgc
     6121 tgtcatttgc cttgacgggt tctgcgccct ccaagaactt gctggatgct gccgtcgccg
     6181 gggagttggt cgatgtggtt aaacgtgaag cgcaagtgga tgcgctgatt gactctgcgc
     6241 tgggcaaggc tcacgtcgag cgcttggccg gcaagtggtt ccgcaccgat ggcctgagcg
     6301 agaagactcg taacgacgcg gaattcaccc cagccgtgcg tcaatccatg atgcaagaga
     6361 ttcgtgagat ctataagcat gttttctaca acggtaattt ccccagcatg tattcagggg
     6421 attacacctt tctggatagc acgctttcca ccttctacaa attgcccggt ggtggcgatg
     6481 cgcctggttc gttccgtatg gtgagcacgg ccaatactca gcgtggtgga gtcatcgcct
     6541 ccggtgctta tatggcgtac aacgcccata tggattacag ctcacctatc cagcgctcgg
     6601 ctcatttccg tcaggatgtg ctttgtcagt ctatcccgct acccgtcaat cttgaggact
     6661 ctcaagagcg tgaagcggct gcgcaattgg tgaaatcgcg ggttgaagca ggtgatatca
     6721 ctacaacgga tttctacgat atccaaacca atatcccaag ctcttcttgt gccacctgcc
     6781 acaacgccat cattaacccg ttgttcggta tggatgactt cgaccacgtt gggcgcttgc
     6841 gtccgaaggt caacaataaa gtggtgcagg atggcctgat attcgtcaat ggtgtagctg
     6901 tgccgaaggg caaggctaat ttggatatca cccaggttaa taatggtagc tacctgtacg
     6961 cctacaatgt tgtcggtact ttatctggcg ctaaagcgga cgctgccaaa gcgtctggcg
     7021 atggcttgaa gttcaacggc gctaaagacc ttggcaaggt gattgtccaa aacgacctgc
     7081 ctggcataag cgcgtgtttg attgaaaaat ccacacgtta cgccctcggt tatagcttag
     7141 acaaagcctt tctggatcct atcgagaacg gacgctatgg aattagtaac acccaggagg
     7201 cggaatttgc gtgtatcaaa aatgagctca acaccgccta tgcacgagcg aagagccccc
     7261 gtgacgttgt gaaagccgct gtcatgtctg atacattccg tttccgaaaa taacaagtgt
     7321 tgctgcggga tcttttgatc ccgctgtaat ggccaacgat aggtttatga atatgaacga
     7381 ttcgaaaaaa ttaccgcaac caaacgacac aattgcggaa gctcagcgca aagatgtgca
     7441 taagtcacgt cgtaaattct tagatttggt tgctaaatcg ggcattggct cggcaacttt
     7501 gaagtactca agtttactgg gtggggtgat gaccgctcgc tatgcctctg cggcagatgc
     7561 tcctaagcgc gttgtctatt tgtatattca cagcggctcg cccgatgatt ggcgcccaag
     7621 ctcggcgtcg gctatatcca ccaaccaatc gtcttatcac tacggtcccg ccagctataa
     7681 cgtcgctcaa atctgccatt tccgcggcgt tgatactgaa gttggggggc atggcggtgc
     7741 tcgtcaggcg ctgggtggtg actattctcg caacacagtt gatgctgatt tgggcgcgat
     7801 tctcggcccc agaagttatt attcatccat ttatctgggc tcgaatgcaa cggattccgg
     7861 taccggggca ggtgtaataa tcagtagtgc tggccagcca gaggatgacc cacggcgcgc
     7921 actaagccga tacttcggtg cagtgccaga gaccagtaaa gacgacactt acatactgtc
     7981 gtttgatgcg cagaaacgtg cgcttgagaa cattaaaaag aagctgagca ccgatgaata
     8041 taaccgagtg caaactcacg ttacggccat cgaaaaaatt gaaagccgta ttgcggatct
     8101 tgccaacgct acagccccag atctaaatgc ctgtaaacca actctacctg gaacctatga
     8161 cgacaccacc aatgccgcca tggttgccca cgcaaagttg caggctgata ttctagttgc
     8221 ggccttcaaa tgtgacttga cccgtgtggg cgtactgcag attgggaacc acaacggcgc
     8281 aggctggacc tataaaggtt ttgatggcca cagcgcggcc cactctggtg gcgcgtctgt
     8341 gtggaaccca atgatgcggg ataaattcga agtgccggct tacttcatca agaaattgat
     8401 ggacacctct gattcaacgg ggcagccgct aattaacagc actgcgtttg tacaggtgac
     8461 ctgtttcggc aatggtctct cccatgcctc taccaatgcg cctttcttat tggcgacaaa
     8521 gatgcccagc tttaagcctg ggttttcatc aaaaggaact gcttccacca gtcgcgattt
     8581 tcacgcggaa gttgctaagg gcttgggggt tgatcccaaa gggcttaagc tcggtggtgc
     8641 caccggtgga gacgtcggtt tggtttaagc gcgactaatc cagcgaaaat taactagtgt
     8701 caaactgaag ccctgccaga ttatttggca gggctttttt gttatggggt tttccaggcc
     8761 cctatttttc tattctccac tttaatctct atggatttac ctttatgacc caatttaatt
     8821 tcccgcctgg aatattggtc tgtccagtat gtgcacaaca agtagtcgct gatcgatgcg
     8881 gcggatgcaa cactgaattt tattccttgg gggaaattcc ctgtctattc accacaggca
     8941 ttcagcaaaa aaacctgtgg cagcatcaaa tggcgatgat ggaatcccaa ggtgccgatg
     9001 cgctcgaaca tctcgactac ataatgcagg gttatgacct atcgcgtcta acccgggatc
     9061 gattggaaca agccagagcc gctatgagcc atagtctcac tgtgattctg gggcagctta
     9121 aaaatgcagg tttgcaaagc gtgcgagacc cggtattcga gcaaatgcgc gtggataacc
     9181 ccaccgagta ttaccaccat attttgcgcg actgggcctg ggacgccgtg cccagccggc
     9241 attttaaaac ccacgttaac gatgccaacc tagagcgcgt gttaaatgta tggccaaatc
     9301 ctgcgcctgg gaaaatgttg tttttaggcg ccggggcggg gcgcttaagt tgggatttac
     9361 accgaatact caagcctgaa tttaccatcg ccgccgatat aaaccccttt ttactcagtt
     9421 gcgcccacag cttaattaaa gcgcgcgaat ctataactct gccagagctt tatacctatc
     9481 cgcagatcgg atttccctac gcaaaatcat ggttgatgga accgccgcag gacccagatg
     9541 gactacatcg caactggtat gccctcggtg ccgatgtctg gaacatgccg ttgcaggaag
     9601 cgttgatgga ttcggttgtc acctcttggt tattggatgt gaccggcgga gacgttaagg
     9661 acctgattgc tgttgtggac tatttgctca aacccggcgg atattggatc aatacaggcc
     9721 ctcttttata ttctgcgcag ctgaatttcg accttaaata ttccgccgag gaaattcttg
     9781 aatttgccga catggctggc tttgatgttg aagagctgag cgttgaggag gctgaacaca
     9841 tggcctcccc cctaaatgcg cgctaccacc atgagcaatt attttctttt tctgcccgca
     9901 aacgaacgca actcaaaccc ttgcctgagc ctggtaacac cagcgagtgg cttacaccag
     9961 cttggttagt gatgcatcat atcccggttc ctaagattca atttaattgc caaattggcc
    10021 atgaatttat tgcgcgggta ttggatctgg ttgacggcaa aagaagcatt tatctgatcg
    10081 ctcaattagt gcaacctcat ttgccggagg gcgtagacgc aaaagaggcg gtggtagctt
    10141 tatttggaca gatattggag ggtatggctg ggctggggga atgacagagc tgcttaattg
    10201 ccatctagct cacaaatgct tacttgctgc cattgttgta cgcactgatt ctgggtttca
    10261 tcccggtaca gcc
//
LOCUS       DAOQGS010000076        10146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3082_length_10146_cov_5.017739, whole genome shotgun sequence.
ACCESSION   DAOQGS010000076 DAOQGS010000000
VERSION     DAOQGS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_10146_cov_5.017739"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..930
                     /gene="tagF"
                     /locus_tag="PKZ52_04415"
     CDS             211..930
                     /gene="tagF"
                     /locus_tag="PKZ52_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HNG59046.1"
                     /translation="MSLTIGLYGKLPGHGDFVARNLPNNLVQIWDGWLQAFIGSSQER
                     LGQDWLNVYLTSPIWRFGFSSGVLDEQQWLGILMPSVDKVGRYFPFSVLVPVPGDLSL
                     LDILCEQSEWLNPVEEAALHALDEGLLVDELLARIGTLNLPTYLRRQWFNQGYQAGPI
                     QVPCADVQMNSAATGLLEGLLATSYSSHSIWTTQGSDRIDACIAIAPGLPAVSSGTAL
                     LDGNWKHWQWMIRMGLNINLA"
     gene            1239..2264
                     /locus_tag="PKZ52_04420"
     CDS             1239..2264
                     /locus_tag="PKZ52_04420"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNG59047.1"
                     /translation="MSEYSLILGGYTDGPGQGIYRMGYNLQQEIFSAAQLVLASHNPS
                     IGLKNGDLWYVVEEAEPGKIHVFDAGNNWQPLLTLNCGGASPCHLACNTGSGHLAVAN
                     YMGGSVSIFALDKNGLPQGAPFIIQHTGRSVTARQEAPHAHYVEFAVNKAGQLGLYAV
                     DLGLDQVLRYPLLVGNQWGAGTIVFKAQAGDGPRHFARHPHNGKLYLLNELSSTVSVI
                     DVADEQWTVQQRISTLPDNYQGENIAAHISISDNGQFIYTSNRGHHSIAVFAVQPDGT
                     LESIQIKPCGGLWPRYFTLLNAAARLIVAHEHSDSVVAFAIEPDGCLRGPQAVGAVPK
                     PTFVAQA"
     gene            3068..3931
                     /locus_tag="PKZ52_04425"
     CDS             3068..3931
                     /locus_tag="PKZ52_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59048.1"
                     /translation="MVIKSVSFSSARSVAALLTLAGIVLHQVMPLKSLSLYPSSAFKS
                     ELYGLKRENGQSSVRWIDESRQLFYCDFKPADPYSCGYTLALGADATHGLDLSDYDGL
                     KLTLRYSGDAPRIRIFLRNYNPVFDKSDDPGLSSKFMAVTIRTADLTASTYVPMKEFS
                     VGDWWIRDFDVPREHSAPEFTNVTYLGVDFVFHSRNEIQVQQIELVGAWIKKETLYLL
                     IILMWLALIVAEGINTGYGLYRKSTLASERIDRLLSEYKNLELQKREFEARSVTDVLT
                     GVLNRAGCSSF"
     gene            3916..4380
                     /locus_tag="PKZ52_04430"
     CDS             3916..4380
                     /locus_tag="PKZ52_04430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG59049.1"
                     /translation="MQQFLKQLFDGDLNGSIGVLLLDVDHFKAVNDQQGHDAGDRVLK
                     EVALLVAHNIRLTDVFGRWGGEEFILICPQMPAERLIALAEKLRIAISEYRFEADRRP
                     LHLSMSIGATLARANESIDTVLKRADLALYEAKNSGRNKVVFKPNMLSEPTL"
     gene            complement(4690..6030)
                     /locus_tag="PKZ52_04435"
     CDS             complement(4690..6030)
                     /locus_tag="PKZ52_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG59050.1"
                     /translation="MQRRSLIKAIAAGSLTAATPLACLLARNALAAGNTAPIRTIFLF
                     HPNGCVPDIFFPKAGSRTLPAMTAPLQSVYDNLVFIDGIGYAGPDYTHEGGAFKCLTG
                     RMTRDDKFSSIDVLMGKEDWANRASNGISVPSVQMGVATFWGNDDTKRISHDNGTTLD
                     SVCDPRIMYPRLFGGGANATGVEAQMLSIAKNDLNRLRSTLGSLEKARMDQHTEALSV
                     LEARFRASSNTALNSCTKPDISPAPQASGLDTDEALSQTDVLDLASGLQQDIAVAALA
                     CGITRTIAFSYGVSVCPVKVPGSQLGDHDASHSDANTHTKSKIWWMGQVAKFIQKLAN
                     TPEGTGSLLDNTILVAVSDLAHGNYHNHYRIPMFLASGKNNNVGLVTGRSLDWRSARA
                     ATAKVQGDQNATDRSINHTDVLDTVRQVAGYTSFQMPNKEGDVLLGWKGNKEPR"
     gene            complement(6047..7996)
                     /locus_tag="PKZ52_04440"
     CDS             complement(6047..7996)
                     /locus_tag="PKZ52_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG59051.1"
                     /translation="MRNSPTRTTLLPIARCVSLLPWAGALLLSLSLAACGSGSAPNNS
                     GTQSSSGSSSSSSTSSSNSSSSSSSSSSSSSSSSSGVVLSGAALYESKGCSGCHGADG
                     LKPSQPIAFNNYSQASLISKIAASMPANAPGSCSGSCAEAVGTYLWSLKPAQAACNNE
                     AALPRRVRLLTKFEYINTINDLFGRTDAGYFAGNVGSDTEVHGFDNNVAANTITLGRM
                     DGFWSAAENAAKTVSLSRYLNSCPSTGAASCFVENFGRRAFRRPLSAEEKADYISLFN
                     QGASRDAGARYVVQTMLVSPNFLYRTELGQSGVLTQYEIASLLSYTFWGSTPDDTLLD
                     KAANNQLANTDQLKQTVDSLLKSTKAQQQFAHFGRQWLRVESVTSVARDAALFPDFNW
                     EIASHMDTELNLFLQEVLLQAGYTMADFVSTNFTFVNPSLEAYYGLPASTGSAFHKVS
                     LTNRPGGILFNGALLTRNAKFFETHPIQRGLFVRNRLLCHQLGTPPPNVGLVAPFNPN
                     LPTRERFARHTANASCASCHQYIDEIGFAFENYTAVGQYRAVESNGAPVDATGRITGL
                     NHLGDTDSHAFNNLPELAQILATEGFNTLSACAAEQFTRLAQGVNEPDSCTLANTVKR
                     WNPSARSLRDLWIEIVAAQSFTQRQ"
     gene            8650..9045
                     /locus_tag="PKZ52_04445"
     CDS             8650..9045
                     /locus_tag="PKZ52_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59052.1"
                     /translation="MKQKRFFLIALITMAACACSSEKHTFEMPVEEVAELKGFILKGV
                     SLSGTVKSGCITNENQLSIKRNGQVIYTEKMRLLQVMSLKDNETFNGEAYTGERIVIY
                     IPDAKKDAILPGDVVISTVSSCANGPVRK"
     gene            9675..>10146
                     /locus_tag="PKZ52_04450"
     CDS             9675..>10146
                     /locus_tag="PKZ52_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNG59053.1"
                     /translation="MNINGVKKFFTKGKSLAALVGVASFMSYSAANACTGVLNNSNNA
                     VGTYNGFYYSFWKQENANRVSVTCGDAGYYKVDWSNVFNWVGGMGWKPGSARIVNYRG
                     TFNSGRNRSSNNSYLALYGWTRPPVEVEYYVVESYGSYNPASCGGSGGVAGGGGS"
BASE COUNT         2391 a         2342 c         2853 g         2560 t
ORIGIN      
        1 acgcgtcgcg cattgagaaa caggcgagcg gcagttacat agtgcgcttt tctgtgccgg
       61 ataccattgt gcaggggtta acccatgata ttgcctatga agtgcggccc aaaagtgcca
      121 ccaatatttt tcggcgggat gtgttaactg gctttagatt gccagaaact ccttaagggc
      181 ctgcgggcca atttaacgct tgaactgatt atgtcgctta ctataggtct ttatgggaaa
      241 ttacccggcc acggtgattt tgttgcgcgc aatttaccca acaatttagt gcaaatttgg
      301 gatggttggc tgcaggcgtt tattggctcc tcccaagagc ggctcggcca ggattggtta
      361 aatgtgtatt taaccagccc aatttggcgg tttggatttt cctcgggtgt tttagatgaa
      421 cagcagtggc tgggtatttt gatgcccagt gtggataagg tgggccgtta ttttcccttt
      481 agtgtgttgg tgccggtgcc gggggattta tccctgttgg atattttgtg tgagcagtct
      541 gaatggttaa atccggtgga ggaagccgct ttacacgctt tagatgaagg cctgttggtg
      601 gatgaattat tagcccgtat cggcacctta aatttaccca cttatttgcg gcggcagtgg
      661 tttaatcagg gttatcaagc gggccccata caagtgccct gcgccgacgt gcaaatgaat
      721 agcgccgcca cgggtttatt ggagggctta ttggccacca gttattccag ccacagcatt
      781 tggaccaccc agggctctga tcgcattgat gcctgtatcg ctattgcacc ggggctgcct
      841 gcggtgagca gcggcacggc gctgctggat ggcaattgga agcattggca gtggatgatt
      901 cgtatggggc tcaacattaa ccttgcttag cagccaattg agcctagcct aataattgcc
      961 ccgctgttaa gcgtggccaa ttccttattc gttcaatcac gctctttaat taactcagcg
     1021 gcaaaataga tcgtcctaat ctattttgcc ctcgccgcac taaacctacg aaaatgcgcg
     1081 gattttcatg ttttagtgcg gctcgcggcg gtttgcacct ttgatgcggc aaccttgccg
     1141 cctttattaa cccggcacta attgccgccg ggttaatata tattcacatt gtctttgcgg
     1201 gcgttattgc gccgcacacc tgtttttgag atttatatat gtctgagtat tcattaattt
     1261 tgggtggtta cacggatggg cctgggcagg gtatttatcg catgggctat aacctacaac
     1321 aggagatatt ttctgctgcc caattagtat tggctagcca caatccctct atagggttaa
     1381 aaaatggtga tctgtggtat gtagtggagg aggcagagcc cggtaaaatt catgtgtttg
     1441 atgcgggcaa caactggcaa ccattattaa cgctcaattg tggcggcgcc tccccctgtc
     1501 atttagcctg taatactggc tcgggccatt tggcggtggc taattatatg gggggcagtg
     1561 tgagtatttt tgcgctggat aaaaacggcc tgccgcaagg agcgcctttt attattcagc
     1621 acacaggccg cagtgtaacg gcacggcaag aggcgcccca cgcccactat gtggaatttg
     1681 ccgtgaataa agccgggcag ttggggcttt atgcggtgga tttaggtttg gatcaggtgt
     1741 tgcggtatcc gcttttagtg ggcaatcagt ggggggcggg cacaatagta tttaaagccc
     1801 aagcgggcga tggcccccgc cattttgctc gtcacccgca caatggcaaa ctctatttgc
     1861 tcaatgaatt aagtagcaca gtttcggtta ttgatgtggc cgacgagcaa tggacggtgc
     1921 agcagcgcat cagtacgctg cctgataact accaaggcga gaatatcgct gcgcacatca
     1981 gtattagcga caacggccaa tttatttata cctccaatcg aggccatcat agtattgctg
     2041 tttttgccgt acagccggat ggtacccttg aatctattca aatcaagccc tgcggtggtt
     2101 tatggccgcg ttattttacg ctgttaaatg cggctgcgcg tttaattgtg gcccatgaac
     2161 attccgattc cgtggtggct tttgccatcg aacctgatgg ctgcctgcgc gggccccaag
     2221 cggtaggtgc ggtccctaaa ccgacatttg ttgcgcaagc ataaccttga gtagagatgc
     2281 aaagtggcct ttgattaagg ctaaaaataa ctattcagca catcttatta ttaacccggc
     2341 ggcatttagt gccgggttca taagctcttt cacgcggacg gcatattttg caggatttag
     2401 ccaggcggca gaatcgatgc gcggtacagc agtgatgaat acaggttatt tcggaccgtc
     2461 aaaatgaggg accttttgac gagcctactt attaacccgg cggcaaaata aatcgtcctg
     2521 atctattttg ccttcgctgc actaaactta cgaaaatgcg cggattttct tgttttagtg
     2581 cagctcgcgg cggctcgcac ctttggcgcg gcatccatgc cgccattatt aacccggccc
     2641 taaatgccgc cgggttaata gaatgaattc acggcgtgtc tgaaataact tgtattgatc
     2701 actgcggtgg gagcattggg tgcttgcagt tacaaggcat tcgtctgata gtttgccgtc
     2761 gatgcgggaa ttacatccag ttacattaaa aaggtgttga ataattaggt ctaaaaaatt
     2821 acctattttc aaaccttgcc agcacttgcc tatagcttaa gttgcagcct ttatacgcgc
     2881 acaatttcag aatattccgc ctcgagacaa aaccgaagaa tccttaagct acaaattacc
     2941 agcggctttg tggcgcataa cacattctgt gtaccagccg atagaaaact gccagtgact
     3001 ttgtgattat ggcggccagc ggccctgctt ttggcgccgt gcgagtgggg cctgtgcagg
     3061 gtaataaatg gttattaaat cggtaagttt ttcctcggcg cggtcggtgg cggcgctgct
     3121 tacactggca gggattgtat tgcaccaggt gatgcccctg aaaagcttat ccctgtaccc
     3181 gagctcagca tttaagagtg agttatatgg ccttaagcgc gagaatggcc aatcctccgt
     3241 gcgctggatc gatgaaagcc gccagctttt ttattgtgat tttaaacctg cagaccccta
     3301 ttcctgcggc tataccctcg ccttaggcgc agatgccacc cacggcttgg atcttagcga
     3361 ttacgacggc ctaaaattaa cgcttcgcta cagcggggat gcgccgcgta tccgcatttt
     3421 tttgcgcaat tacaacccag tgtttgataa aagcgacgac ccgggtttat ccagtaaatt
     3481 tatggcggtg actattcgca ccgccgattt aactgcatcc acctatgtgc cgatgaaaga
     3541 atttagtgtg ggtgattggt ggatccgcga ttttgatgta ccacgggagc attcggcgcc
     3601 ggaattcacc aatgttactt atttgggtgt cgattttgtt tttcacagcc gcaatgaaat
     3661 tcaggtgcag caaattgagt tggtaggcgc ctggatcaaa aaagaaaccc tgtatttgct
     3721 gattattttg atgtggctgg ccttgattgt tgccgagggt attaatactg gctacggcct
     3781 ctaccgtaaa tcaaccctgg catccgagcg catcgaccgt ttattgagcg agtataaaaa
     3841 tttagaactg caaaagcggg aatttgaagc caggtcggtg acggatgtat taaccggtgt
     3901 gttaaatcgc gcggggtgca gcagttttta aaacaattat ttgatggcga cctgaatggc
     3961 tctatcggtg ttctgttgct cgatgtcgac cactttaaag cggtgaatga ccagcagggg
     4021 catgacgcgg gcgatcgcgt attaaaagaa gtggcattgc tggttgcaca caatatccgt
     4081 ttaaccgatg tatttgggcg ctggggcgga gaggaattta ttttgatttg cccgcaaatg
     4141 cccgccgagc gtttaattgc cctggcggaa aaacttcgca tcgccattag tgaatatcgt
     4201 tttgaagcgg acaggcggcc gctgcattta tccatgagca tcggcgcaac cctggcccgt
     4261 gccaatgagt ccatcgacac agtattaaaa cgggcggatt tagcattata cgaggctaaa
     4321 aatagcgggc gaaataaggt ggtttttaaa cccaatatgc tcagtgaacc tacgctttaa
     4381 tcgaaaaatt gccgttaaac cctactgtgc ccgcagtggt gactagaggt taacgcagac
     4441 acgccgtgca tacatccgtg taggctcgtc aaaaaggtcc ttctttttgg cggtctgcac
     4501 tacctctagt caccattgct acaccgcgaa tcgattgtgc cgcccgcgcg gacgagggta
     4561 ataaggtgtg ctaaacagtt aaattcggcg tcttaaacat tctcgctacg ggttcaactc
     4621 tggatgttag gtttaaacgc attggtaact gaggctgtga ggttaaccga gggttttgcc
     4681 gagccatttt taacgcggct ctttattgcc tttccaaccg agcaatacat caccctcttt
     4741 attaggcatt tgaaaactcg tataaccggc cacctgtcgc actgtatcca gcacatcggt
     4801 gtggttgata ctgcggtccg tggcgttttg atcgccctgt actttcgccg tggcggcgcg
     4861 ggcgctgcgc cagtctaagg agcggccggt gaccaagccc acattattat ttttgccgct
     4921 ggctaagaac atagggatgc ggtagtggtt gtggtaattg ccgtgggcaa ggtccgacac
     4981 cgccaccaaa atggtgttat ccaataaaga gcctgtgccc tccggcgtat tggcgagttt
     5041 ctgaataaat tttgccacct ggcccatcca ccaaattttt gatttggtgt gggtgttggc
     5101 gtccgagtgg gaggcgtcgt gatcgccaag ttgggagccg ggcactttca ccgggcagac
     5161 ggacacgccg tagctaaagg cgatggtgcg ggtgatgccg caggcgaggg cggctacggc
     5221 tatgtcctgt tgcaggccgg aggctaaatc cagcacatca gtttgtgaga gggcttcgtc
     5281 tgtatccagg cctgaggcct ggggagcagg gctgatatcg ggcttggtac agctgttaag
     5341 ggcagtatta ctgctggcgc gaaagcgcgc ttccaatacc gacagcgctt ccgtgtgctg
     5401 atccatgcgg gctttttcca ggctgcctaa agtgctgcgc aggcggttta aatcgttttt
     5461 ggcgatggac agcatctggg cttccacgcc cgttgcattg gcgccgccgc cgaataaacg
     5521 cgggtacatg atgcgcgggt cgcagacgga atccagggtg gtgccgttgt cgtggctgat
     5581 gcgtttggtg tcgtcgttgc cccagaaggt ggccacgccc atttgcaccg acggcacact
     5641 gatgccgttg ctggcccggt tggcccaatc ctctttaccc atcaacacgt cgatggagga
     5701 gaatttatcg tcccgggtca tgcggccggt taggcattta aaggcgccgc cctcgtgggt
     5761 gtagtcaggc cccgcgtagc cgataccgtc gataaacacc aggttgtcat aaacgctttg
     5821 caagggcgcg gtcatggcgg gcagggtacg gctgccggct ttaggaaaga aaatatccgg
     5881 tacacagccg ttggggtgaa agagaaaaat agtccggatg ggggctgtat tgcctgcggc
     5941 cagggcgttg cgggccagca agcaggctaa tggcgtggcg gcggtgaggg agcctgcggc
     6001 tatagcctta atgagtgaac gtctttgcat ggggcgaatt cctaggctac tggcgctgag
     6061 taaaagattg ggcggcgacg atttcaatcc acaggtcccg cagggagcgg gcactgggat
     6121 tccagcgctt gacggtattg gcaagggtgc agctatccgg ctcgttcacg ccctgggcta
     6181 agcgggtgaa ctgctcggcg gcgcaggcgg agagggtgtt aaacccctcg gtggccagga
     6241 tctgggcgag ttccggcagg ttgttaaagg cgtggctgtc ggtatccccc aggtggttaa
     6301 ggccggtgat gcggcctgtg gcatccaccg gggcgccgtt ggattccacc gcccggtact
     6361 ggcctaccgc cgtgtaattt tcaaaggcaa agccgatttc atctatgtac tgatggcagg
     6421 aggcgcagct ggcgttggcc gtgtggcggg caaagcgctc gcgggtgggc aggttcgggt
     6481 taaacggcgc cactaggccc acattggggg gcggggtgcc cagctggtgg cacagcaggc
     6541 gattgcgcac aaacaggccg cgctgaatcg ggtgggtttc aaagaacttg gcgttgcggg
     6601 tgagcagagc gccgttaaaa agaatgccgc cgggccggtt ggtaaggctc actttatgga
     6661 atgcgctgcc cgtgctggcg ggcaggccgt aataggcctc taagctgggg ttaacaaagg
     6721 tgaaattggt agagacaaaa tccgccatgg tgtaaccggc ctgcagcaag acttcctgca
     6781 ggaacaggtt caattcggtg tccatatggc tggcaatttc ccaattaaaa tccgggaaga
     6841 gggcggcgtc gcgggccacg ctggtgactg actccacccg cagccattgg cggccaaaat
     6901 gggcaaactg ttgctgggct ttggtgcttt ttaacaggct atcgacggtt tgtttaagct
     6961 gatccgtgtt tgccaattga ttgttggcgg ccttatccaa aagcgtgtca tcgggggtgg
     7021 agccccaaaa ggtatacgaa agcaggctcg ctatttcata ttgggtcaat acaccgcttt
     7081 gcccaagctc cgtgcggtac aaaaagttgg gggagaccaa catggtttgc accacgtagc
     7141 gggcaccggc gtctcggctg gcgccttggt taaacaggct gatgtaatcg gctttttcct
     7201 ctgcacttaa gggccggcga aaggcgcggc ggccaaaatt ctccacaaaa caactggcgg
     7261 cgccggtgct ggggcagctg tttaaatagc gggagaggga aaccgttttg gcggcatttt
     7321 cagccgcgct ccaaaaccca tccatgcgcc cgagggtaat ggtgttggcc gccacattgt
     7381 tatcaaagcc gtgcacctcg gtatccgagc ctacgttgcc agcaaaatag ccggcatcgg
     7441 tgcggccaaa taaatcgttg atggtgttga tgtattcaaa cttggttaac aggcgcaccc
     7501 ggcggggcag ggccgcttca ttgttgcagg ccgcttgggc gggctttagg gaccacaaat
     7561 aagtgcccac cgcctcggcg caactaccgc tacagctgcc cggcgcattg gcgggcatgg
     7621 aggcggcgat tttgctgatt aaactggcct gggagtaatt gttaaacgcg atgggctggc
     7681 tgggtttgag gccgtcggcg ccgtggcagc cgctacagcc tttggattca taaagggccg
     7741 caccacttag aacaaccccc gaggagctgc tgctggatga acttgaagaa cttgaactcg
     7801 acgaactgga attcgaggag cttgtactgg acgagcttga actgccgctc gaactttgtg
     7861 tgcccgaatt attcggagcg gagccgctgc cgcaggcggc caggctcagg cttaacagga
     7921 gcgccccagc ccagggcaag agcgatacgc agcgcgcgat ggggagcagg gtggtacggg
     7981 tcggtgagtt tctcatgagc aaaacgccta aacgggctga gcggtgaata atgggcagag
     8041 gcttagggct ggtggccact acacggctgg gcggccgcta caaagcgggt tattggcaaa
     8101 ttcttaccct cgtgtccgat gccgagggct tagccagttc aatctgatta ggctcagtgt
     8161 aaaccctaat cacaccaagg cttgtagcca gtttgaatat ttgcgaacgc aatcagtact
     8221 ttttactgag tattgaacgc cgaacccttt acatcccatt accttcaaat aatcagacaa
     8281 aggtatgtgg ttgtcagtgg taaatggtat ggggggctag gcagtggcag gtttagccgt
     8341 tcacattcaa cgcagtggga atactgaatg aggcgctcat ggggggtaaa ttcggcagct
     8401 ctcgtgaatg aaaacgaggc aatgcccaaa tagcgcattg tttaacaaaa agaattgtgc
     8461 atttgggcag cctgtacaag ggacaaaata aagtcggtac tatttccgcc ttggatctgg
     8521 ggctgtttac tttggctcgg tatttttcat cgtgtttgtg atttgcacat tggggcggag
     8581 ccaagaaaga atccagctgt gtaaactgat ccgctcaaca aatttagcta aagaaatgcg
     8641 agtaaaacaa tgaagcaaaa acgattcttc ttaattgcac taataacaat ggcagcctgt
     8701 gcatgtagtt cagaaaagca tacctttgaa atgcccgtgg aagaggttgc agagttaaaa
     8761 gggtttattt taaaaggtgt ttctttgtct ggcacagtca aatccggctg tatcactaat
     8821 gaaaatcaat tatcgatcaa gcgcaatgga caggtaattt acacagaaaa aatgcgttta
     8881 ttgcaagtta tgagcctcaa agacaatgag acttttaatg gcgaggccta cacaggtgag
     8941 cggatcgtta tatacattcc agacgctaaa aaagacgcta tcttgcctgg tgatgtagtc
     9001 attagtactg tttcttcctg tgcaaatggg ccggttagaa agtaatttca caattcatat
     9061 ggattttgtt gcttggtaca aaagtgggaa aacgcctggc tgcaaacggt ttcaaatgaa
     9121 taaatattgg cttaattttc cgggcaaaca tttattcggt gggtaaaagt caattaatcg
     9181 acatggcaat gtaatttttc acgctaataa gagatgtttt gcgtaaatta aattgtcaga
     9241 ccggaaccaa ttcacagcgg tgattaagag gtctgcgtta agctgaacgg aaatctaaag
     9301 gtgaaaacgt atctcgtagt tcgcaagact atatgaggga gatcgttaaa cctaggctgg
     9361 tgaggcatcc gcgaacgtga aaagcgctta aggtaattga aaataacagc attaagcggc
     9421 gggtaaaatt ctggataaaa catctcttgc agcgattttt ttcttcgcct ctcaattaat
     9481 aaattgcatt ggctgaagat tggaaaaact aaaagaaacg cccaaaaaca ggccagatac
     9541 tttgggaaat gtagtgacat gcgaaagtca gcaggctggc ttaagcagat aacttaaaca
     9601 gataattaag gcatgcccta aagcgcgcac attaaaccgg caaaacagat caatcgatat
     9661 aaaaaggggt tcctatgaac attaacggtg tcaaaaaatt ctttacaaag ggaaaaagcc
     9721 tagcggctct ggtaggggtg gcgtctttta tgagctactc tgctgcaaac gcctgtaccg
     9781 gcgtgcttaa caactcaaac aacgctgtgg gcacatacaa cggcttctat tattcgtttt
     9841 ggaagcagga gaatgccaac cgagtgagcg ttacctgtgg agatgccggc tactacaagg
     9901 ttgattggag caacgtattc aactgggttg gcggcatggg ttggaagcca ggttcggcga
     9961 gaatcgttaa ttaccgcggt acgttcaaca gtggccgcaa ccgcagttcg aacaactctt
    10021 atcttgcgct ctatggctgg actcggcctc cagtagaagt tgagtattac gtagttgaga
    10081 gttacggttc ctacaacccc gcaagctgcg gcggcagcgg cggtgttgct ggcggtggtg
    10141 gcagtg
//
LOCUS       DAOQGS010000077        10128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3090_length_10128_cov_4.813164, whole genome shotgun sequence.
ACCESSION   DAOQGS010000077 DAOQGS010000000
VERSION     DAOQGS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_10128_cov_4.813164"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="PKZ52_04455"
     CDS             complement(<1..1841)
                     /locus_tag="PKZ52_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNG59054.1"
                     /translation="MGEQRNYAALDWVVGEIGETLKEARQALEAFVEDPRDVSRLRFC
                     LTHIHQVLGSLQMIEFHGAALITEEVELLAQSLIHNTVTNVHEAQEVLMRALLQLPIY
                     LEHVQRFKDDHPGVLLPLLNDIRAVRKQPFLSESGLFTPDLSRLHHIQDERHPVLQDR
                     AKLIQALKKLREMYQYAAASLLKDIKISENLEYIDKVCSRVELMSRGTRAFALWQAAC
                     GVVENLRADEIELSAAVRSLLRRLARELKILAESAPAAFDAPVDENLLRNLLYYTARA
                     ERLGPHGQTLRQKYHLEKALNDGAETGRHFHKTMISAPDPEAIRSVIAALQEELNTVK
                     HILGMTLAGQAGVSDMEEILPIMKRLADTLAVLAIPELRKFAQDQYDQLAHSCRTGSI
                     DPEQLANLAGRIVEIEHKLDAVSKSAGKRDLANLDGRQIEVDSAKETVIRESRLGLEQ
                     CKEGIVEFYASKWDRNYLAAAPDLLANIRGGLSMIGEGRAAAALDACSRFIQEQFLSV
                     EYIPDAASLTALANALEGVDYYLERILEPTVVSDVTRFLILAEDNLRQLSYPAKGRER
                     AEPAAAAEGAKTPGPSPYKHLIAQADLPPAQVILAENNQDEGADSAPV"
     gene            complement(1859..2704)
                     /locus_tag="PKZ52_04460"
     CDS             complement(1859..2704)
                     /locus_tag="PKZ52_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNG59055.1"
                     /translation="MIWSLQAVPEISDQEFALWSKLLEERAGIYLTAQQRTFLQTQVV
                     MRMRELGLSDFTSYYQLIVDGVAGMVEWSRLIDRLVVKETSFFRHTPSYEFVAQHLAS
                     HLGQAAQHSYEVWSVGCSTGEEPYSLAMVLAEGFAAAHREPLFGITATDISRAALSLA
                     KNGIYSERKLDFIPQALRQKYLLAVEDGRFQIHRSLRERLCFNQANLLDINDMPAVQV
                     DLIYCQNLLVYFRRWLREKILNAFVQRLKPGGALVIGLGEVLDWSHPSMQRAPFDAAQ
                     AYIHK"
     gene            complement(2724..4952)
                     /locus_tag="PKZ52_04465"
     CDS             complement(2724..4952)
                     /locus_tag="PKZ52_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG59056.1"
                     /translation="MKIESSSLISRFKDNPALSILIVLLGCFGVLIAVMIYIFLRDAK
                     QDAEHERHVAELRAYSYRLTALSRDATAGNKAEFKTLRQVISDMDKTWKALQSADNET
                     KVQLADQLAAFESVWSRTKQSSEVITNNEDSIILMHDVAKTLNEALPELQAEHDNIVE
                     LLLENKASAEQVSVAQRQSWFAERIGRNVDRMLRGGLDADKAADEFKLDASRFGKVLE
                     AMKNGDASMGIAKITNTQAVESLDRISKLFAFVSGSIKDIYEKSPDLFSARQSTNAIL
                     DGSPLLQQRLTELSDQIKSLPSKRKVNNTAILFVVGCAFVLLVIIGLLLNNATNKRLK
                     ITAETNERNQNAILRLLDELADLADGDLTATATVTEDFTGAIADSINYTIDQLRVLVS
                     RINDTSANVSAASEETRQTALHLARASEHQAQEIAGASAAVNEMAVTIDQVSANAAES
                     ARVAERAVSIAFNGAKVVQNTIHGMDTIREQIQDTAKRIKRLGESSQEIGDIVSLIND
                     IADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSATATKQIEALVKTIQNDT
                     TAAVNSMEQTTAEVVRGARLAQDAGVALEEIEAVSANLAELIQNISNAARQQSTSAGH
                     ISNTMNVIQEFTSQTSAGTSATATAIGRLAQMAIELHESVAGFKLPEEMNSAAGKRLR
                     AAPSAAANLAPAAVAAATVAAVSHGPEETEAEFEFSLDEDVFADGGDGVNPFATDDLR
                     KT"
     gene            complement(5059..5604)
                     /locus_tag="PKZ52_04470"
     CDS             complement(5059..5604)
                     /locus_tag="PKZ52_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNG59057.1"
                     /translation="MTDASAFDALTSLVSLSKQHAVGLPAQVDTTPRWSGIGFSLLGR
                     KFIAPMRQIAELLEVPASITRLPGVHPWVVGLSNVRGRLLPLFDMAYFFGGKSLGHRK
                     QQRVLVIETEAYFSGLIVDQAFGMQHFTADKFNETVEAVPEALAPCVRGSYTDPSGIS
                     WAVFDMGTLVKDPRFTNASLT"
     gene            complement(5612..5974)
                     /gene="pilH"
                     /locus_tag="PKZ52_04475"
     CDS             complement(5612..5974)
                     /gene="pilH"
                     /locus_tag="PKZ52_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilH"
                     /protein_id="HNG59058.1"
                     /translation="MARVLIVDDSPTETYKLTSMLEKHGHQVLTAGNGADGVALAKAQ
                     LPDVVLMDVVMPGLNGFQATRQLNKAPETTKIPVIIVTTKDQETDRVWGMRQGAKAYL
                     TKPIEEKELISVIAHVSN"
     gene            complement(5994..6389)
                     /gene="pilG"
                     /locus_tag="PKZ52_04480"
     CDS             complement(5994..6389)
                     /gene="pilG"
                     /locus_tag="PKZ52_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HNG59059.1"
                     /translation="MDVNLEHLKVMVIDDSKTIRRTAETLLKKVGCTVVTATDGFDAL
                     AKIADTRPDIIFVDIMMPRLDGYQTCALIKNNSEFKSTPVIMLSSKDGLFDKAKGRIV
                     GSDQYLTKPFSKGELLGAIEAHVKTTSAG"
     gene            complement(6484..6639)
                     /locus_tag="PKZ52_04485"
     CDS             complement(6484..6639)
                     /locus_tag="PKZ52_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59060.1"
                     /translation="MKAPAIAVVNEQLNRRQAKITSHRLTLAVGVGAKFVNLTYFLDI
                     QILMESP"
     gene            6763..7719
                     /gene="gshB"
                     /locus_tag="PKZ52_04490"
     CDS             6763..7719
                     /gene="gshB"
                     /locus_tag="PKZ52_04490"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNG59061.1"
                     /translation="MTQLRTLAVVMDPIEGINFYKDTTLALLLAAQQQGFELWYAQLQ
                     DLYAQNGSAYGSLRQLQVFQDANQWYQLGERREVPLGDIDAILMRKDPPFDNQYIYAT
                     YILEQAQHQGALVVNNPRSLRDCNEKFFATHFPACCPPHLVSRDIARLRAFHAEHQDV
                     IYKPLDGMGGSRIFRAKADDPNVSVILESLTQDGREYIMAQRYIPEITQGDKRILVVD
                     GEPIPYALARIPAQGETRGNLAAGGKGVPVPLTERDHWIVQQVAPSLKEKGLLFVGLD
                     VIGDYLTEINVTSPTCAREINKVYPTDIGSLLVNAITRRLGS"
     gene            7729..8601
                     /locus_tag="PKZ52_04495"
     CDS             7729..8601
                     /locus_tag="PKZ52_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNG59062.1"
                     /translation="MATLDPTVETGDRLSFTLFLAIGLHALVLLGVGFTVVHGSKVAP
                     TLNVTLATHSTEAPKEADFLAQTNQEASGTADKPKELTAAEDAVFADPNVQQTTPIEM
                     HKTTEANSNPDEVVKTQADSSQRVASNDNPTLEPAAETREADDIETPPETAEKAALMA
                     KRDRLNQQEAMRPRIRRMTAVSAIASKDAEYLNQWNETVEAVGNRHFPKEALNNRQFG
                     ELRLAVQINPNGTIDHVEILEGAPHSILNQAALQIVHLAAPFKAVPAEVMAGNDKLEI
                     IRTWKFEITGLSTN"
     gene            8695..9273
                     /locus_tag="PKZ52_04500"
     CDS             8695..9273
                     /locus_tag="PKZ52_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNG59063.1"
                     /translation="MPIESSISFGDNLRNHLLIATPSLKDPHFSHTVTYICDHSAEGA
                     MGIVLNRPLDLNIGQVFEELNLPLGERICQAPVLSGGPVNTQRGFVLHNEEGQWDSSL
                     KVTDGICLTGSKDILTALAVGQGPTHALFALGHAGWSAGQLEDELATNAWLTVPASYE
                     LLFCTPIELRWSAAAKHLGIDPNLISPQLGHG"
     gene            9279..9719
                     /gene="ruvX"
                     /locus_tag="PKZ52_04505"
     CDS             9279..9719
                     /gene="ruvX"
                     /locus_tag="PKZ52_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNG59064.1"
                     /translation="MDRPRSALLFDFGLKNIGLAYGQTISQTARELPALKARDGIPQW
                     EAIGALLKEWQPNLLLVGLPLNMDGSESELSGRARTFGNRLNGRFNMPVEMVDERLST
                     REAKWEAATRGHKGNYREAPIDSIAARIILEAWLRNTPADIAGP"
BASE COUNT         2160 a         2961 c         2801 g         2206 t
ORIGIN      
        1 acaggggcac tgtcagcgcc ctcgtcctgg ttgttttcag ccagaataac ctgcgcaggg
       61 ggcagatccg cctgagcaat caaatgtttg tacggcgacg ggccaggtgt tttggcccct
      121 tcggcggccg ccgcaggctc ggcgcgctcg cggcccttgg cggggtaact gagctggcgc
      181 aggttgtctt ccgcgaggat caaaaagcgc gtcacatcgc tcaccacggt gggctcaagg
      241 atgcgctcta agtaataatc gaccccttct aacgcattgg ccagggcggt taaggaggcg
      301 gcgtcgggga tgtattcaac gcttaaaaac tgctcttgga taaaccggct gcaggcatct
      361 agggccgcag cggcgcggcc ttcgccgatc atactcaagc cgccgcgaat attggccagc
      421 aggtccggcg cggcggccag gtaattgcga tcccacttgg aggcgtaaaa ctccactatg
      481 ccttctttgc actgctccag gcccaaacgg ctctcgcgga tgaccgtttc tttggcgctg
      541 tccacttcga tctggcggcc gtcgaggttg gccagatccc gcttgcccgc gcttttgctg
      601 accgcatcga gcttgtgttc gatctccact atacggccgg caagattggc cagctgctcc
      661 gggtcgatgc tgccggtgcg acagctgtgg gccagctggt cgtactggtc ctgggcgaac
      721 ttgcgcagtt cagggattgc cagcaccgcc agggtatcgg ccaggcgctt cataattggc
      781 aggatttctt ccatatccga cacccccgct tgccctgcga gggtcatgcc cagtatgtgt
      841 ttaaccgtgt tgagctcttc ctgcagggcg gcgatgacgc tgcggatggc ctctgggtcc
      901 ggggcgctga tcatggtttt gtggaagtgg cggccggttt cggcgccgtc attgagggcc
      961 ttctccaggt ggtacttctg ccgcagggtt tggccgtggg ggcctaaacg ctccgcccgc
     1021 gccgtgtaat acaacaggtt gcgcaacagg ttttcatcca ccggcgcatc gaaggccgcc
     1081 ggggcgcttt ccgccagtat tttcaattcc cgcgccaggc ggcgcagcag gctgcgcacg
     1141 gcggcggaga gttcaatttc atcggcgcgc aggttttcca ccaccccgca ggccgcctgc
     1201 cagagggcaa aggcgcgggt gccccggctc atcagctcca cccgggagca gaccttatcg
     1261 atgtactcca ggttttcgct gattttgatg tctttaagca ggctcgccgc ggcgtattgg
     1321 tacatctcgc gcagcttttt gagggcttga atcaacttgg cgcgatcctg cagcaccggg
     1381 tggcgctcgt cttgaatgtg gtgcaggcgg cttaaatccg gcgtaaacag gccggattcg
     1441 cttaaaaacg gctgcttgcg caccgcccga atatcgttga gcaggggcaa taacacgccg
     1501 gggtggtcgt ctttaaaacg ctgcacatgc tctaggtaga taggcagttg cagcagggcg
     1561 cgcatcagca cttcttgggc ttcgtgcacg ttggtaacgg tgttgtgaat caacgactgg
     1621 gccagcagct ccacctcttc ggtgatcagg gccgcgccgt ggaattcgat catttgcagc
     1681 gagcctaaca cctggtggat atgggttaga caaaaacgca gacgtgacac atcccgcgga
     1741 tcctccacga aggcttcaag ggcctggcgc gcctctttga gggtttcgcc tatttcacct
     1801 accacccagt caagggctgc gtagttgcgt tgttctccca ttgatggagt cccgttcttt
     1861 atttgtgtat ataagcttgt gccgcgtcga agggcgcgcg ctgcatggac ggatgggacc
     1921 agtccaatac ctcgcccagc ccaattacca acgcgccgcc gggtttaagc cgctgcacaa
     1981 aagcattgag aattttttcg cgcaaccagc ggcgaaagta aaccaacagg ttttggcagt
     2041 aaatcaaatc cacctgcacc gctggcatat cgtttatgtc caacagattg gcctggttaa
     2101 aacacagccg ctcgcgcagg gagcggtgta tttgaaagcg cccatcctcc accgccaaca
     2161 agtacttctg ccgcagggcc tggggaataa aatccagttt gcgctcgctg tagatgccgt
     2221 tcttcgccag cgacagggcc gcccggctga tgtcggtggc ggtaatgccg aataggggct
     2281 cccggtgggc agcggcaaac ccctccgcca gcaccatagc cagggagtag ggctcctcgc
     2341 cggttgaaca ccctacgctc cacacctcat aactgtgctg ggctgcctgc cccaggtggg
     2401 aagctaagtg ctgggcgaca aattcgtagg acggcgtatg ccgaaagaag ctggtttctt
     2461 ttaccaccag gcggtcgatc aggcgcgacc actccaccat gcccgccacg ccgtccacga
     2521 tcagctggta gtaactggta aagtcgctca agcccagctc gcgcatacgc atcaccacct
     2581 gggtctgcaa aaaggtgcgc tgttgggcgg ttaaataaat accggcgcgc tcctctagga
     2641 gtttgctcca aagggcaaac tcctggtcgg aaatctccgg tactgcctgc aatgaccaga
     2701 tcatattcta ggctggcgca gccttaggtc ttacgcagat cgtcggtggc aaaggggttc
     2761 acaccgtcgc caccgtctgc aaatacatct tcatccaacg aaaactcaaa ctccgcctcg
     2821 gtctcttcag gcccgtggct cacggcggct acagttgctg cagccacggc ggcaggggct
     2881 aggttggcgg ccgcactggg ggcggcgcgc aggcgtttac cggcagcgga gttcatctcc
     2941 tcgggcaatt taaagcccgc caccgattcg tgtaactcga tggccatctg cgccaagcgg
     3001 ccgatggccg tagcggtggc gctggtaccg gcggaggttt gtgaggtgaa ctcttggatc
     3061 acgttcatgg tgttggagat gtggccagcg gaggtcgatt gctggcgagc ggcgttggag
     3121 atgttttgga tcaattcggc caggttcgcc gataccgctt cgatctcttc cagagcaaca
     3181 cccgcgtctt gcgccagacg ggctccgcgc accacttctg cggtggtctg ctccatggag
     3241 ttcaccgccg cggtggtgtc gttctgaatg gtttttacca gggcttcgat ctgtttggtg
     3301 gcggtggctg agcgttccgc caggcgctgt acctcgtccg ctaccaccgc gaagccacgg
     3361 cctgcatcgc ccgccatgga tgcttggatc gccgcgttca aagccagaat gttggtttgg
     3421 tcggcgatgt cgttgatcag gcttacgatg tcgccgatct cctgggagga ctcacccaag
     3481 cgtttaattc gtttagcggt gtcttgaatc tgctcgcgga tggtatccat accgtggatg
     3541 gtgttctgta ccaccttggc accgttaaag gcgatggata ccgcccgctc cgccacccgg
     3601 gcggactcag cggcgttggc cgatacctgg tcgatggtga ccgccatttc gttcaccgcc
     3661 gccgaagcac cggcgatctc ctgggcctgg tgctccgagg cgcgggctag atgcaaggcg
     3721 gtttgacggg tttcttccga ggctgccgat acgttggcgg aggtgtcgtt aatgcgcgac
     3781 accaacaccc gcaattggtc gatggtgtag ttgatagagt cggcgatggc gccagtgaaa
     3841 tcctcggtca ccgtggcggt ggcggtgaga tccccgtccg ccaggtccgc caattcatcg
     3901 agcagacgca aaatcgcgtt ctggttgcgc tcgttggttt cggcggtgat ttttaagcgt
     3961 ttgttggtgg cgttgttcaa caacaagccg atgatcacca gcagcacaaa cgcgcagccc
     4021 accacaaaca ggatggcggt gttattcact ttgcgcttgg agggcaggct tttgatttga
     4081 tcagacagct cggttaagcg ctgttgcagc aatgggctgc cgtccaaaat ggcgttggtg
     4141 gactggcggg cgctaaagag atccggcgat ttctcgtaga tgtctttaat ggagccactt
     4201 acgaaggcga agagtttaga gatgcgatcg aggctctcca ccgcctgggt gttggtgatt
     4261 ttggcgattc ccatgctggc gtcgccgttt ttcatggctt ccagcacctt accgaaacgg
     4321 ctggcgtcca gtttgaattc atcggcggcc ttatccgcat ccaaaccgcc gcgcaacatg
     4381 cggtctacgt tccggccgat gcgctcggca aaccaggatt ggcgctgggc caccgatacc
     4441 tgctcagcgg aggctttgtt ctctaacagc agctcaacga tattgtcgtg ttcggcctgt
     4501 agttccggca gggcttcgtt aagggttttg gccacatcgt gcatgaggat gatggaatcc
     4561 tcgttgttgg tgattacctc agagctctgc ttggtgcggc tccacacgct ttcaaacgcc
     4621 gccagctggt cggccagctg taccttggtt tcgttatcgg cgctttgcag agccttccag
     4681 gttttatcca tatcggaaat cacctggcgc agggttttaa attccgcctt gttgccggcg
     4741 gtggcgtccc gggagagggc cgttaggcgg taggagtagg cgcgcaactc agcaacatgc
     4801 cgctcgtgtt cagcatcctg tttcgcatcg cgcaggaaga tgtaaatcat caccgcgatc
     4861 agaacaccaa aacaccctaa taagacgatt aaaatcgata aggccggatt atctttaaat
     4921 cgcgatatca agctgctgga ttcaattttc ataggttgct ccgctggtta caccgcccga
     4981 attgccctgt gtgtgctggc attcagtcat ttattgttag taaagcaatg ctttagcacc
     5041 gcttctatct cgtgttgttt aggtcagaga ggcgttggta aaacgcggat ccttcactaa
     5101 ggttcccata tcaaaaacgg cccaggagat accggatggg tctgtgtagg agccgcgcac
     5161 gcagggcgcc agggcctccg gtaccgcctc caccgtttcg ttgaatttat cggcggtaaa
     5221 gtgctgcatg ccaaaggcct gatcgacgat caagccgctg aaataagcct cggtttcgat
     5281 cactaatacc cgctgttgct tgcgatggcc gagggatttg ccaccaaaaa aatacgccat
     5341 atcaaacagt ggcaagaggc gcccgcgcac attggaaagt cccaccaccc atggatgcac
     5401 accgggcagg cgggtaatac tggcgggcac ttccaacaat tctgcaatct gccgcatggg
     5461 ggcgataaat ttgcgcccca gcagggaaaa accgatgccg ctccaacggg gcgtagtatc
     5521 cacctgggca ggcaggccca ccgcatgttg tttggagagg gagactaggc tcgtcagggc
     5581 atcaaaagca gaagcgtcgg tcataggcgg cctagttgct gacgtgggcg atcacactga
     5641 tcagttcttt ttcttcgatg ggcttggtga gataggcctt agccccctgc cgcatacccc
     5701 atactcggtc ggtttcctga tccttagtgg tgacaataat gaccgggatt ttggtggttt
     5761 ccggggcctt attgagctgg cgagtggcct gaaagccatt gagccccggc atcaccacgt
     5821 ccatcagcac cacatccggt agctgggctt tggccagggc gacgccatcg gcgccattgc
     5881 ccgcggtaag cacctggtgg ccatgtttct ccagcatact ggtgagtttg taggtttcag
     5941 tgggtgaatc atcaacaatc aagacacgcg ccatggttac ctccgatcct taatcagcct
     6001 gcgctggtgg ttttaacatg ggcttcaatt gcaccgagca attcgccctt gctaaagggt
     6061 ttggtcaagt actgatcgga gccgacgatg cgccccttgg ccttatcaaa caagccgtct
     6121 ttactggaca gcatgatgac cggggtggat ttaaattcgc tgttgttttt gatcagcgcg
     6181 caggtttgat aaccatccaa acgcggcatc atgatgtcga caaaaataat atccgggcgg
     6241 gtatcggcga tttttgccag ggcgtcaaag ccgtctgttg cggtgacaac ggtgcagccg
     6301 actttcttta acaacgtctc tgcggtgcga cgaatggttt tgctatcgtc gatgaccatc
     6361 accttaagat gttctagatt cacgtccata gacagactct ataccagtgg ttttaataaa
     6421 ttatcgccat agaaagacta tccatcatag ccttttggcg ggcttgctct tttaacacag
     6481 ttgtcagggc gattccataa gtatttgaat atcaagaaaa tacgtcaaat taacgaattt
     6541 agcgcccacg cccaccgcta aagttaagcg atgagacgta attttggcct gccgccgatt
     6601 taattgctca ttcactacag ctatagcagg tgctttcaaa cgataccttt tataatcgac
     6661 caactttagc tattccgcca cgcggccagc agcttttttc gccctctggc aacccttaca
     6721 cagcaagcct tggaacccta aggcgatttg cgaggaaaac ctatgacaca gcttagaaca
     6781 cttgccgtag tgatggaccc catcgagggg atcaatttct acaaagacac tacgctcgct
     6841 ttattattgg cagcccagca acagggtttt gagctgtggt acgcccagct gcaggacctt
     6901 tatgcgcaaa atggtagcgc ttatggcagc ctgcgccaac tgcaggtatt tcaggatgcc
     6961 aaccagtggt accaattggg cgagcggcgg gaagttcctt taggcgacat agacgccatt
     7021 ttgatgcgca aagacccgcc gtttgataac cagtatatct acgccactta tattcttgaa
     7081 caggctcagc accagggcgc cctggtggtg aacaacccgc gcagcctgcg cgactgcaac
     7141 gagaagtttt ttgccaccca tttccccgca tgttgcccac cccatttagt cagccgcgac
     7201 atagcccgcc tgcgcgcctt ccacgccgaa catcaggatg tgatttacaa acccctagac
     7261 ggcatgggcg gcagccgcat cttcagagcc aaagccgacg accccaacgt cagcgttatt
     7321 ctggagagcc tcacccagga cggccgcgaa tacatcatgg cccagcgcta catacccgag
     7381 atcacccagg gcgataaacg cattctggtg gtggacggtg agcccatccc ctatgccctg
     7441 gcccgcattc ccgcccaggg cgaaacccgc ggcaatctcg ccgctggcgg caagggcgtg
     7501 ccggtgccgc tcaccgagcg ggatcattgg attgtgcagc aggtcgcgcc tagtcttaag
     7561 gagaagggat tactctttgt aggcctggac gtgatcggcg attacctcac tgaaatcaac
     7621 gtcaccagcc ccacctgtgc ccgcgagatc aacaaggtct accccacgga cataggcagc
     7681 ttgttagtca acgccatcac tcgacgctta ggatcttaac ctgcacccat ggccaccctc
     7741 gacccgactg tagaaaccgg cgaccggtta agctttaccc tcttccttgc catcggcctg
     7801 cacgccctgg tgctgttggg tgtagggttt accgtggtgc acggctctaa agtggcgccc
     7861 actctcaacg taaccctcgc cacccacagc accgaagccc ccaaagaggc ggatttttta
     7921 gcccaaacca accaagaggc cagcggcacc gccgacaaac ccaaggagct gaccgccgcc
     7981 gaagacgccg tatttgccga tccaaacgtg cagcagacaa cccccatcga aatgcacaaa
     8041 accaccgagg ccaacagcaa cccggacgag gtagttaaaa cccaggccga cagcagccag
     8101 agggtggcca gcaacgacaa tcccacccta gagcccgccg ccgagacccg cgaggccgac
     8161 gacatcgaaa ccccgccgga aaccgctgaa aaagccgccc tcatggccaa gcgggaccgc
     8221 cttaaccaac aggaggccat gcgcccgcgc attcgccgca tgacggcggt ctccgccatc
     8281 gcctccaaag acgccgaata cctcaaccag tggaacgaaa ccgtagaggc cgtgggcaat
     8341 cgccacttcc ccaaggaggc cctcaacaac cgccagtttg gcgagctgcg cctggcggtg
     8401 cagatcaacc ccaacggcac catcgaccac gtggagattc tcgagggcgc cccccacagc
     8461 atcttgaacc aggcggcctt gcaaatcgta catttggccg caccctttaa agcagtgcct
     8521 gcggaagtga tggccggcaa tgacaagctg gaaatcatcc gcacctggaa atttgaaatt
     8581 accgggctct ccaccaatta gcccgctatt gttttaatga acagacagcc gttttagtga
     8641 attcgcaaaa aggggccctg ggcccgcaag gctctaaggg cctgggagtt ttacatgccg
     8701 attgaatcca gcatcagctt tggggataac ctgcgcaacc acctgttgat cgccaccccg
     8761 agcctgaaag acccgcactt ttcccatacc gtcacctata tttgcgacca cagcgccgag
     8821 ggcgccatgg gcatagtgtt aaaccgcccg ctggatctca acatcggcca ggtatttgaa
     8881 gaattaaacc tgcccttagg cgagcgcatc tgccaagcgc cggtgctctc aggcggcccg
     8941 gtgaataccc agcgcggctt tgtgctacac aacgaagagg gccaatggga ctccagcctt
     9001 aaggtcaccg acggcatttg cctgaccggc tcgaaggata tactcaccgc cctggccgta
     9061 ggccaaggcc ccacccacgc cctctttgcc ctcggccacg ccggctggag cgcaggccaa
     9121 ctggaggacg agctggccac caacgcctgg ctcaccgtgc ccgcctccta tgaattgctg
     9181 ttttgcaccc cgattgaatt gcgctggagt gcggcggcca aacacctagg catagacccg
     9241 aacctcatct ccccccaatt gggccacggc tagtggcggt ggatagaccc cgctcggccc
     9301 tgttatttga cttcggcctt aaaaacatcg gcctggccta tggccaaacc atcagccaaa
     9361 ccgcccgcga actgcctgcc ttaaaagctc gcgacggcat cccccagtgg gaggctattg
     9421 gcgccctgct caaagaatgg cagcccaacc ttttgctggt gggcttgccg ctgaatatgg
     9481 acggcagtga aagcgaactg agcggccgcg cccgcacctt cggcaaccgt ttaaacggac
     9541 gctttaatat gccggtagaa atggtggacg agcgcctctc cactcgggaa gccaaatggg
     9601 aggccgccac ccgaggccat aaaggcaact accgagaagc acccatcgat tccatcgcgg
     9661 cgcggattat tttagaggct tggttaagaa atacgcctgc cgacatcgca gggccataat
     9721 gcattcaggc tgttactgct cttaatagct cgcggccgaa taaagccaat taattcgttt
     9781 caattaaaat ccgcacaaac acgctagcca attaaactga caagggcttt tattagcaaa
     9841 ttaacgagaa aactcctagg tgcaattttt accattccga tacattttgc acccgaggct
     9901 taaaaacgcc gagttattaa gctttgaatt tccacaagaa cattttttcg ttcaaacttt
     9961 tattccatca aagtgggcat accggccggt aaaattttgt taccccagcc ccacgcacga
    10021 tatttttggc ggcgaagcgt tttattggtc tgcacggctt taaataaaaa aaggggctct
    10081 tcgtagaatt gtgtaaaacc cccgcgccag atggcaaaat gaccttcc
//
LOCUS       DAOQGS010000078        10117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3093_length_10117_cov_4.965514, whole genome shotgun sequence.
ACCESSION   DAOQGS010000078 DAOQGS010000000
VERSION     DAOQGS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3093_length_10117_cov_4.965514"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2545)
                     /gene="mprF"
                     /locus_tag="PKZ52_04510"
     CDS             complement(<1..2545)
                     /gene="mprF"
                     /locus_tag="PKZ52_04510"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HNG59065.1"
                     /translation="MNTKTLLTGLSVLLCSLIIGLAAWQWHPVALSDIYQSFLQISLR
                     QWCLAALFTCASYGFSLLGDYWHLRHEPQLSRRDLVLAGFIGETFSQNTAGLARQWDS
                     SRYRLYPDSPASKQAISQLKRQNSASFILGFISLWFATQTIALANQQLFFSGSFLLQL
                     LFTAAAALALIALALLRFSAYTPTLLPADWCQSVKQLKLYSIATAVAHIVCAAAVLWQ
                     LMPSAYLPHTLHLSAYLAALTASIVGVTPAGVGVFEGSYWLLLGGYQGPSLIAALLAY
                     RLMYHIIPLCFSGVFLFVYEWRTVYSSRSSVQRFLRFAHLPLAVVNYSFPKILSLLLL
                     IAGLILLVSGNTSALDDRLEVLMDIIGLPFIELSHLVGSIVGVVLLFLARAVSLRIRS
                     AYYITVYLLCLGIVASLLKGIDYEEALALLAVLALLIPSKHHFYRDSALNIHLLNRHW
                     WILISVIVAFSVWVGFFCYKEVEYSHELWWQFELDSHASRFLRAQLLLIVLGLCGAIW
                     FFTQKKPYQAPLPSEEEVLAAESLVQQSANSDHFLALTGDKFYFWSATQKAFIAYIPT
                     AAYWYAMSDPCGDAAEFPALISAFREAADKVGAKIVFYRVTTAHLPIYIDMGLHLVKL
                     GEEAKINLAEFHTQGAARASMRNLLNKMPRDGWQFEVIPAEKFGAIWPAIAEISKAWL
                     HEKNAKEKSFSLGRCDYNYLSCCDTAIVRNAERIVAFMNILRPDNKSEMSIDLMRYHP
                     DAPKSVMEYLTLSVIFWAKAEGYQAFNLGMAPLSGLDNDRLASLWHKVGAYVYTFGSE
                     FYGFVGLRAYKEKFAPLWEPRYLAVPSSLHLPKILLSLVALISGAPKNSR"
     gene            complement(2624..2851)
                     /locus_tag="PKZ52_04515"
     CDS             complement(2624..2851)
                     /locus_tag="PKZ52_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010645784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNG59066.1"
                     /translation="MASPKTLGPHEYLWINDECINCDMCVPQCPSESIALAEKHYQIN
                     PATCIGCEDYYHTPTCIEVCPIDCILILPTA"
     gene            complement(2838..4133)
                     /gene="yegQ"
                     /locus_tag="PKZ52_04520"
     CDS             complement(2838..4133)
                     /gene="yegQ"
                     /locus_tag="PKZ52_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNG59067.1"
                     /translation="MSHTELLSPAGSLKALRLAFAYGADAVYAGQPRFSLRVRNNEFD
                     LDNLASGINTAHDLGKKLYVVTNIAPHNAKLQNFIGDMAPVVDLKPDAMIVSDPGIIA
                     LMREHFPHMPLHLSVQANTMNWAAVKFWQSIGIERVILSRELSINEIAEIKQRCPEIE
                     LEVFVHGALCMAYSGRCLISSYVNKRDANQGACTNACRWNYQTHSAQESEQDLIPVQT
                     YTPEPKSIFVEQAEHPGELFALEEDEHGSYLFNSKDLRAVEQVEKLIAAGVDSLKIEG
                     RTKSPYYVARTAQVYRRAIDDASAGRAFNPELLSELDGLANRGYTAGFLQRHTPVSLQ
                     NYQQGSSRGESQQLVGEVIAQLGPQELQIDVKNRFTQQDQLLLLTPEGNYRFNPEAII
                     NKQGDTIEVAPGSGHIVRLMVPHSVNGEHAMLVKIHGEP"
     gene            complement(4202..5020)
                     /locus_tag="PKZ52_04525"
     CDS             complement(4202..5020)
                     /locus_tag="PKZ52_04525"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNG59068.1"
                     /translation="MPTPLTDTRAPMPVVYLPHGGGPLPLLQDPAHQSLSLYLQSLGQ
                     LPTPEVILIISAHWEAPSAQFTKAERPELIFDYSGFPPETYQYHYPAPGAPERAAAWA
                     ERLNAAQIAAGTSARGWDHGCFVPLLLSHPQANIPVVQLSLIRGLNPSEHIALGAALA
                     PLRHEGVLIIGSGLSFHNMAVFFAPSPSNRERSEAFDTWLNESLCGEHLSHQQRCERL
                     INWQAAPQARFCHPREEHLLPLHVCLGAAQASPQAQRSYSDILMGVKVSGFTWW"
     gene            5276..5890
                     /locus_tag="PKZ52_04530"
     CDS             5276..5890
                     /locus_tag="PKZ52_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG59069.1"
                     /translation="MDLIKFFQSGGFFMYPILLVMTLGIAIAIERFIYLVVTQRATEA
                     LWKRLLPMLKARDVERAHQAVENQKTPLARVFNYGFARLLNRASRETVEAAMEEGVME
                     VTPLMQERTHYLATLANIATLLGLLGTIIGLIDAFTAVSTADPAQKAELLSASIAVAM
                     NTTAFGLVAAIPLLLMHSFLATKTAKLIDNVEMATVKCLNLTME"
     gene            5896..6477
                     /locus_tag="PKZ52_04535"
     CDS             5896..6477
                     /locus_tag="PKZ52_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG59070.1"
                     /translation="MLKFSRKNRLEQVADIDVTTFMNLMVILVPFLLVTAVFSRMTVL
                     QLQLPSKDAESGAAAPVDIQLQLELTPSEWILRSNDTDEITRLNAPHNYNEWRRFEEA
                     LVEIKSRFPEEKAITLFFDRQVPYKSMLEVMDHVRQTQIVTGAQAQWVELFPEIALGR
                     HEPAEPAVAAPSEQAPAAEPAAPPAPGAGEAQP"
     gene            6474..6968
                     /locus_tag="PKZ52_04540"
     CDS             6474..6968
                     /locus_tag="PKZ52_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG59071.1"
                     /translation="MSQSRRARRMERHHQLAKGPGLNLVSLMDIFTILVFFLLVNSAG
                     PQNLPSLKDLRMPSVVSLNPPAETLVLAVTKERVLLQGEPIGAWQQWQTDGPALLQAL
                     GQALQQNAAKTSAPEQGRPITLMADESVPYEVIEKLIDLCKEQDYRQIAFAANLKAAP
                     QGQP"
     gene            6968..7966
                     /locus_tag="PKZ52_04545"
     CDS             6968..7966
                     /locus_tag="PKZ52_04545"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNG59072.1"
                     /translation="MAQSATLALHRYGLDWQPNNSSDRTFYLIAGLCLGASLLLSLVI
                     ANTQVPPKPVQTQAPVAPRIVNYMANRPKPVIDIPPPKPKPAPIVAPAKPKPRTEPAP
                     ASVVQQPVQPVEPAPTASLGRNRPAATTTDPNKASARAKAQSAGLLALGNQVASVLDT
                     APSTQALAQTASTQTAQAASTTNVTDHIGSNRKLTLDANATATATSTRALDQQAVQAS
                     AQQLKQSLNTAAAGPATSGTGRSDKDIAGVFAQNQGGLNSLYERARRQIPGLAGKLVL
                     ELSIAADGSVSAIKVLASELNNPELEGKIISRVKQFKFAAGKAQNAVKYAMEFVPG"
     gene            complement(8214..9857)
                     /locus_tag="PKZ52_04550"
     CDS             complement(8214..9857)
                     /locus_tag="PKZ52_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNG59073.1"
                     /translation="MKKHSFQTACLALVSTLALNNCLLAAETSEVSREQVDNAVKQLT
                     QDAANIIEKGCGASNAGGTQREKAGVNCKIPGLAVAVVYKDEVLYAGGFGVREINTVC
                     ATKNDSAAFKAQCAVNADTVFQLASVSKPISASVVAALISQKRITWDSKLADLTPEFH
                     MYDPWVTSEITIRDMFSHRSGLPKHANDLLEDLDYGRAAMLSHLRFIKPASSFRSTYA
                     YTNAGLTQGAIAAATAYGLDWADAAEQTIYKPLGMASTSSRFKDFMARPNKATNHMLV
                     DGQWRHKEQRNPDAQSPAGGVSSSVNDLTKWMRMELNKGKFENNQVVAEEPLAETQFP
                     QIVSGKNHNHQPTFYGLGWGVAYDEQKRTYLSHSGGFALGAATHLLLIPEAQLGLVVL
                     TNAAPIGVAEGLAKNFTDSVFYGKPQNNWLHIFTEVFKDPKVIGIETLSNYTVRPTKP
                     TPAQKNSAYSGRYGNNPWIGDVVIEEHNGKLALIIGPNAKKLPLSHFDRDTFTFATVG
                     ENAAGLSGVTFTMGADGKAQSLLIESLNQQGQGQLARLP"
BASE COUNT         2515 a         2625 c         2639 g         2338 t
ORIGIN      
        1 cgcgcgaatt tttaggtgca ccggaaatta gcgccaccaa acttaataat attttaggca
       61 aatgcaggct ggagggcacg gctagataac ggggctccca taatggggcg aatttttctt
      121 tataggcgcg caggcccaca aagccataaa actcactgcc aaaggtataa acataggcac
      181 ccaccttatg ccacagggag gctaggcggt cattatcaag acctgacagg ggcgccatcc
      241 ctaaattaaa cgcctggtag ccttcagctt tggcccaaaa aataacgcta agggtgaggt
      301 attccatcac cgatttaggc gcatccgggt ggtagcgcat taaatctatc gacatttcgc
      361 ttttattatc cggtcgcaaa atattcataa aggctactat gcgctctgca ttgcgtacta
      421 tggcggtatc gcagcaggat aaataattgt aatcacagcg gccgagggaa aaactttttt
      481 ccttggcgtt tttctcgtgc agccaggctt tggatatttc ggcaatggcg ggccaaattg
      541 cgccgaattt ttctgccgga ataacttcaa actgccaacc gtcgcggggc attttattta
      601 atagattgcg catggaggcg cgggccgccc cctgggtgtg aaattccgcc aggtttattt
      661 tcgcctcttc gccgagtttt accaggtgta agcccatatc gatataaatc ggcaaatggg
      721 cagtagtaac gcggtaaaaa acaattttag cgcccacctt atccgccgct tcgcgaaagg
      781 cgctaataag tgcgggaaat tctgcggcat cgccgcaggg gtcactcatg gcgtaccagt
      841 aagccgctgt gggtatatag gcgataaagg ccttttgtgt tgccgaccaa aaataaaact
      901 tatccccagt gagggcgagg aaatggtctg aattggctga ttgttgcacc aggctttctg
      961 ctgctaatac ttcttcttcg ctgggcaggg gcgcttggta gggttttttc tgggtgaaaa
     1021 accagatagc gccgcacagg cctaaaacga ttaataataa ttgcgcgcgc aaaaagcgcg
     1081 aggcgtggct gtccaactca aattgccacc acagctcgtg gctgtattcc acttctttgt
     1141 aacagaagaa ccctacccaa actgaaaacg ccacaatcac actgatgagt atccaccagt
     1201 ggcgattaag caggtgaata tttaatgcgc tgtcccggta aaaatggtgt ttgctcggaa
     1261 ttaacagggc gagcacggct aacaaggcca aggcttcttc gtaatctatg cctttaagca
     1321 gagacgcaac aatacctaag cacaataaat acaccgtaat gtagtaggcg gagcgaatgc
     1381 gcaggcttac agcgcgggct aaaaataaca acactacacc cacaatactg cctaccaggt
     1441 gggacagttc aataaacggc aggccgataa tatccatcaa cacttccagc cgatcatcca
     1501 gggcgctggt attgccagag actaataaaa tcagcccagc aattaacaac aataaagaga
     1561 gaattttcgg gaaactataa ttaaccaccg ccaagggtaa atgggcaaag cgcaaaaagc
     1621 gctgcacgct gctgcgggat gaataaaccg tgcgccattc gtagacgaat aaaaacacgc
     1681 cggagaaaca cagggggata atgtgataca tcaagcgata agccagcagg gctgcaatca
     1741 ggctgggccc ttggtagccg cctaataaca gccagtagct gccttcaaaa acgccgacgc
     1801 cagccggtgt tacgccgaca atactcgccg tcagtgccgc taaataggcg gataaatgca
     1861 gggtatgcgg caggtacgcg ctgggcatta attgccacaa caccgctgcg gcacacacaa
     1921 tatgcgccac cgccgtggcg atagaataca gttttaattg tttaacactc tggcaccagt
     1981 cagcgggcaa aagcgttggt gtgtaggcgc taaagcgcag tagggcgagg gcgataagcg
     2041 caagtgcggc ggcagcagta aatagcagtt gcagtaaaaa actgccgcta aaaaataatt
     2101 gctggttggc aagggcgatg gtttgggtgg caaaccacaa actgataaac cctaaaataa
     2161 aactggcgct attctggcgt tttaattggc tgatggcctg tttactcgcg ggggaatctg
     2221 ggtagagcct atagcgggag gaatcccatt ggcgggctaa gcctgcggta ttttgactaa
     2281 atgtttcgcc gataaatcct gccaatacta aatcccgccg cgataattgc ggctcgtggc
     2341 gcaggtgcca ataatcgccc agcagggaaa acccgtagga ggcacaggtg aagagtgctg
     2401 ctaaacacca ttggcgcaga ctaatctgta aaaagctttg ataaatatcg ctcagtgcca
     2461 ccggatgcca ttgccatgcg gcaaggccga tgatcaggct acaaagtaaa acagatagcc
     2521 cggttaggag cgttttggta ttcatctaag gcctctttgg gcggtttttg tagcggtaaa
     2581 tggccccatc ataaaaaaca gcagccggtg ataaacaggc cgcttaagct gtgggtaaaa
     2641 ttaaaataca gtcgataggg cagacttcaa tacaggtggg tgtgtggtaa tagtcttcac
     2701 agccgatgca ggtcgccggg ttaatttgat agtgtttttc agccaaggcg atggattcac
     2761 tggggcattg gggtacacac atatcgcagt ttatgcactc gtcgttaatc cacaagtatt
     2821 catgggggcc gagggtttta gggctcgcca tggattttca ccagcatggc gtgttcgcca
     2881 ttaacactgt ggggaaccat taaacgcaca atatggccgg aaccgggggc tacttctata
     2941 gtgtcgccct gtttattaat aattgcctcg gggttaaagc gataattacc ctccggtgtt
     3001 agcagtaata attgatcctg ctgagtaaaa cggtttttca catcaatctg taattcctgc
     3061 ggccccagtt gggcgatgac ttcacccact aattgctggc tctctccacg gcttgagccc
     3121 tgctgataat tttgcagaga cacaggtgtg tggcgctgta aaaagcctgc ggtgtagccg
     3181 cgattggcca ggccgtctaa ttcgctgagc aattccgggt taaaggcgcg cccggcgctg
     3241 gcatcgtcaa tggcgcgccg gtacacctgg gcggtgcggg ccacataata aggggatttg
     3301 gtgcggcctt cgattttaag tgagtccacc ccggcagcga tgagtttttc cacctgttcc
     3361 accgcgcgca gatctttgga attaaataaa taactgccgt gttcatcctc ttccagcgca
     3421 aataattccc ctgggtgttc ggcctgttcc acaaaaatac ttttaggttc cggcgtatag
     3481 gtttgcacgg gaattaaatc ctgctcggat tcttgcgcgc tgtgggtttg ataattccag
     3541 cggcaggcat tagtgcaggc gccttgattg gcatcgcgtt tattcacata actggagatt
     3601 aaacagcggc cggaataggc catacataag gcgccgtgca caaatacttc caattctatc
     3661 tccgggcagc gctgtttaat ttcggcaatt tcattaatgc ttaattcgcg ggataaaatc
     3721 acccgttcaa tgccgatgct ctgccaaaat ttaacggctg cccaattcat ggtgttggct
     3781 tgcacggata aatgcagcgg catatggggg aagtgctcgc gcattaacgc gataatgcct
     3841 gggtcggaaa caatcatggc gtcgggtttt aaatccacca ccggcgccat atcaccaata
     3901 aaattctgca atttggcgtt gtggggcgcg atattggtga ccacatataa ttttttgccg
     3961 aggtcgtggg cggtattaat cccgctggct aaattatcca aatcaaattc gttattgcgt
     4021 acccgcaggc taaaacgcgg ctgcccggcg tacacagcat cggcgccgta ggcaaaggcc
     4081 aggcgcaagg cttttaaact gcctgcgggg gataataatt cggtgtgcga cataaaaaat
     4141 acactagatg gggcgcaaag cgcttaaaaa agcggcggat tttacgcgcc ttgccaatgg
     4201 gttaccacca agtaaaaccg ctaaccttga cgcccatcaa aatatcgctg tagctgcgtt
     4261 gggcttgagg gcttgcctgt gcggcaccca ggcaaacatg caggggcaac aggtgctcct
     4321 ccctcgggtg gcaaaagcgc gcctggggcg ccgcctgcca attaattaag cgctcacagc
     4381 gctgttggtg gcttaaatgc tcgccgcaca ggctctcgtt cagccaggta tcaaaggcct
     4441 cgctgcgctc gcgattgctg ggcgagggcg caaagaacac agccatatta tgaaaagaca
     4501 acccagagcc aatgattaac accccttcat ggcgcaaagg cgccagggcc gcacctaggg
     4561 caatatgctc agaaggattc agcccgcgaa tgagggatag ctgcaccacc ggaatattcg
     4621 cctgcgggtg gctcagcagc agcggcacaa aacaaccgtg gtcccagccg cgcgcggagg
     4681 tgcccgcggc aatttgggcg gcattaaggc gctctgccca ggccgccgcg cgctcaggcg
     4741 cacctggcgc cgggtagtgg tattggtagg tttccggtgg gaagccgctg taatcaaaaa
     4801 ttaattccgg ccgctccgct tttgtaaatt gtgcactggg cgcctcccag tgggcgctta
     4861 tgataagtat cacctcaggt gtgggcagct ggcccaggct ctgtaaataa aggctgaggc
     4921 tttgatgggc tgggtcttgc agcaggggca gggggccgcc gccgtggggt aaatacacca
     4981 caggcatggg ggcgcgagtg tcagtaagcg gcgttggcat agggggtgtg ctccagtgga
     5041 attaatcggt tagcacctgc agtatcggct tttttcgcag taaataaagg ttatttttcg
     5101 ccgccgggaa gggttttgct ggccgaggca ttgatttaga atgcccgggc ttttaaggct
     5161 tggcctgtaa ttggcaaggc aattttgatc cccagccgag ggacacgagt ttccaaggcc
     5221 ccccactttc ccctctgtcg cgtcgcgcat gttttgagtt tttaaggtaa aaattatgga
     5281 cctcattaag ttttttcaat cgggtggatt ttttatgtac ccgattttat tggtcatgac
     5341 cctcggcatc gcaatcgcga ttgaacggtt tatttattta gttgtgaccc agcgcgccac
     5401 cgaagcactc tggaagcgtt tattacctat gttaaaagcc cgcgatgtag agcgcgccca
     5461 ccaagcggtg gaaaaccaaa aaacaccttt ggcccgcgta tttaattacg gttttgcgcg
     5521 tttattaaat agagccagcc gggaaacagt agaagcggcg atggaagagg gcgtgatgga
     5581 agtaacccca ttaatgcaag agcgcaccca ctacttagct acccttgcca atattgcgac
     5641 cctattaggc ctgcttggca ccatcatagg tttgatcgac gcctttaccg cagtctccac
     5701 tgcagaccca gcgcaaaaag ccgaattatt atcggccagt atcgccgtgg ccatgaacac
     5761 caccgccttt ggcctggtag cagccattcc gctgctatta atgcactcgt ttttagccac
     5821 taaaacagcc aaattaattg ataacgtaga aatggccacg gttaaatgcc ttaacctcac
     5881 catggaataa accggatgtt aaagttttcc cgcaagaatc gcttggagca agtggcggat
     5941 attgatgtca ccaccttcat gaatttgatg gttattttgg tgccgttttt gttggtcact
     6001 gcggtatttt cccgcatgac ggtgctgcaa ttacagctgc cttccaaaga tgccgaaagt
     6061 ggcgccgcag ctccagtgga tattcagcta cagttggaat taaccccttc cgagtggatc
     6121 ttgcgctcga atgataccga tgaaattacc cgtttaaatg cgccacacaa ttacaacgag
     6181 tggcgccgct ttgaagaagc cctagtggaa attaaaagcc gctttcctga ggaaaaagcc
     6241 atcaccttat tttttgaccg ccaggtgccc tataaaagca tgctggaagt tatggaccat
     6301 gtgcggcaaa cccaaattgt taccggcgcc caggcccagt gggtggaatt attccccgag
     6361 atagcccttg gccgccatga gcctgctgag cccgcagttg ctgcccctag tgaacaagca
     6421 ccagcagccg aaccagcagc gcccccggcg cccggggccg gggaggcaca gccatgagtc
     6481 aatcccgtcg cgcccgccgc atggagcggc accatcaact ggccaaaggg ccgggcctta
     6541 atctcgtttc cttgatggat attttcacca tcctggtgtt tttcttactg gtgaattctg
     6601 ctggccctca gaacctgccc agccttaaag acctgcgcat gccctcggtg gtgagcctca
     6661 accctccggc agaaaccctg gtgctggccg tcaccaaaga gcgggtgctg ctgcagggtg
     6721 agcccatagg cgcctggcag cagtggcaga cggacggccc ggcactgtta caagccctgg
     6781 ggcaggctct gcaacaaaac gctgctaaaa cctcggcccc tgaacagggc cgccccatca
     6841 ccctgatggc cgatgaaagc gtgccctatg aagtgatcga aaaacttatc gacctctgta
     6901 aagaacagga ttaccggcaa atagcctttg cggccaattt aaaagccgcc ccgcaaggac
     6961 aaccctaatg gcccaatctg caacacttgc gctgcaccgt tacggcctgg actggcagcc
     7021 caacaacagc agcgaccgca ctttttacct tattgccggg ctgtgtctgg gcgccagcct
     7081 gttgttatcg cttgtgatcg ccaacaccca ggtgccgcct aagcctgtgc aaacccaagc
     7141 gccggtggcg ccgcgcatcg tcaattacat ggccaaccgg cccaagccgg tgatcgatat
     7201 tccgcccccc aagcctaagc ccgcccccat tgtggccccg gccaagccca aaccccgcac
     7261 ggagcctgcg cccgcttctg tggtgcagca gccggtgcaa ccggtggagc ctgcgcccac
     7321 tgcaagcttg ggccgcaacc gccctgcggc caccacgacg gacccgaata aagcctccgc
     7381 ccgcgccaaa gcgcaaagcg caggcctttt agccctgggc aaccaggtgg caagcgtgct
     7441 ggatacagcc cccagcaccc aagccctggc ccaaaccgcc agcacccaaa ccgcccaggc
     7501 ggccagcacc acgaatgtta ccgaccacat cggcagcaac cgcaagctca ccctagacgc
     7561 caatgccaca gccaccgcca ctagcacccg cgctttagat caacaggcgg tacaggcctc
     7621 cgcccagcag ctcaaacaat ccctcaacac cgccgccgca ggccccgcca cctccggcac
     7681 cggccgtagc gacaaggaca tagccggtgt atttgcgcaa aaccaaggag gccttaacag
     7741 cctctacgag cgcgcccgca ggcagatacc gggtttggcg ggtaaattag tgctggaact
     7801 gagcatcgcc gccgacggca gcgtaagcgc cattaaagta ttagccagtg aactgaataa
     7861 cccggagctg gaaggcaaaa ttattagccg ggtaaaacaa tttaaattcg ctgcgggcaa
     7921 ggcgcagaat gcggttaaat atgcgatgga gtttgtgccg gggtgagttt aatcctccaa
     7981 tctgcaaaaa atataaacgg ctaagggcgc gcaattgtga agccgtgccg cttaaaagca
     8041 atttaattgc ctgcgtgcta gctggctgca caattaattt tgcaggtgtt gtttattttt
     8101 aaatggccca cctaaccgtc tgttaaacta ccttgtcagc ttgttgccgc caaggtagtt
     8161 caacaggctg ctaagctgta tcagcgctgg catttaaaat tttttgcaat tcattacggt
     8221 aaacgcgcca gttgcccctg cccctgttga tttaaactct caatcagcag gctttgcgct
     8281 ttgccgtcgg cgcccatggt gaaggtaact cccgacaggc ccgctgcatt ttcacctacg
     8341 gttgcaaatg taaaagtgtc ccggtcgaaa tgagacaaag gtaatttttt ggcattgggg
     8401 ccgataatta gcgctagttt gccattgtgc tcttcgataa caacatcgcc aatccacgga
     8461 ttatttccat atcgaccgct gtaagccgaa tttttttgcg ccggtgtagg tttggttggg
     8521 cgaacggtgt agttggataa tgtttcgatg ccgataactt tagggtcttt aaaaacttca
     8581 gtgaaaatgt gcagccaatt attttgtggt ttgccgtaaa aaacgctgtc ggtaaaattt
     8641 ttggccaaac cttctgcaac acctatgggt gctgcgttgg ttaaaaccac taggcccaat
     8701 tgcgcttctg gaattagcag taggtgcgtt gccgcgccta acgcaaaccc gccagaatgg
     8761 gaaagatagg tacgtttttg ttcgtcgtac gcaacgcccc agcctaaacc ataaaacgtc
     8821 ggttgatggt tgtgattttt acccgacaca atttgcggaa attgtgtttc cgcaaggggc
     8881 tcttccgcaa caacttggtt attctcaaat ttccccttat taagctccat gcgcatccac
     8941 ttggttaaat cattgacgga cgaactcacg ccaccggctg gagattgcgc atcaggattg
     9001 cgttgttctt tatgccgcca ctggccatcc accagcatat gattggtagc cttattgggg
     9061 cgcgccataa aatccttaaa gcgggagctg gttgaggcca tgcctaaggg tttatatatt
     9121 gtttgttcgg cagcatcggc ccagtcgagc ccataggcag tagccgctgc aatagcacct
     9181 tgcgttaagc ctgcgttggt atacgcgtaa gtggagcgaa aactgctggc gggttttata
     9241 aaacgtaaat ggcttaacat ggccgcgcgc ccataatcca aatcttccaa taaatcgtta
     9301 gcgtgcttgg gtaaaccgct gcggtgggaa aacatatcgc gaatggtgat ttcactggtt
     9361 acccaagggt cgtacatgtg aaactcgggg gtcaaatcgg caagtttgct atcccaggta
     9421 atacgttttt ggctaattaa agcggccaca actgatgcgc taatgggttt tgaaacagag
     9481 gcgagttgaa acacggtgtc agcattaacg gcgcattgtg ctttaaaagc cgcgctgtca
     9541 ttttttgttg cacaaacagt attaatttca cgcacaccaa agccaccggc gtaaagcact
     9601 tcatctttat agaccacagc taccgccagg ccaggaattt tgcaattcac accagccttt
     9661 tcccgttgtg taccaccggc attgcttgcc ccacaaccct tctcaataat attcgccgcg
     9721 tcttgtgtta actgcttaac ggcattatcc acttgctcgc gggagacctc agaggtttcg
     9781 gctgcgagaa gacaattatt gagggcgagt gttgatacaa gggcgaggca ggctgtttgg
     9841 aaactatgct ttttcatttt gaagccttag atgataggcg ttggcgggca tggtggttgg
     9901 taattaacgg gttgggcagt ctaacaaagt tggcagagga ttatgctcgc caggcttgaa
     9961 aaatccgcag ggagctctgg taaaagttgg gctagcccgt aggttggaag agcgcagcga
    10021 cttccgacat tcggtgctcg ctccaggcat taacccctaa gcgtcggaag tcgctgcgct
    10081 cttccaacct tgtatctctc agcaaagcgg attaact
//
LOCUS       DAOQGS010000079        10102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3106_length_10102_cov_4.537076, whole genome shotgun sequence.
ACCESSION   DAOQGS010000079 DAOQGS010000000
VERSION     DAOQGS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3106_length_10102_cov_4.537076"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(757..1494)
                     /locus_tag="PKZ52_04555"
     CDS             complement(757..1494)
                     /locus_tag="PKZ52_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59074.1"
                     /translation="MKQSCEVNSVWSDAESELILWNEQQRGLGRIELPGRSGLFHSAL
                     LGVDIVENQLLIDMPFPSPPIDLVLPHTQCKLSFIKQQQLLQLVVQVEERLLYEGRTA
                     LLVKILQREFHCDRRAGARIGFARGQWPLVRMQIPMVEQVRARVINLSSGGALLNIFG
                     RHDTSIQAKAQVAMKLELTDGTCIDVKAAIKSVSYYRRPCQHTQFRVQFLAMSERDAR
                     KLDLFIAYQLDQAGKEITFASELEAAY"
     gene            1759..2568
                     /gene="ttcA"
                     /locus_tag="PKZ52_04560"
                     /pseudo
     CDS             1759..2568
                     /gene="ttcA"
                     /locus_tag="PKZ52_04560"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            2689..3126
                     /locus_tag="PKZ52_04565"
     CDS             2689..3126
                     /locus_tag="PKZ52_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG59075.1"
                     /translation="MSKKIIVPLVTLLLAGAGTLSLKPCIIPGPICEAHMEKVGAQKD
                     KDNPYYLSFTTPWLAQRANILYPAYAMDRGLTGVTELELRIDSDGKLLEKSISESSGH
                     PILDKAALAAADTFVFNVAGFGNVLFPYSKELKIRFRLDDGPH"
     gene            complement(3263..3481)
                     /locus_tag="PKZ52_04570"
     CDS             complement(3263..3481)
                     /locus_tag="PKZ52_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HNG59076.1"
                     /translation="MNRSGESGSVPARHNRFFQQHDYWYYRTREGIDIGPFDNQEEAQ
                     RGAREFIDYVVNHNPDFIDILSRYKAAA"
     gene            3849..4679
                     /locus_tag="PKZ52_04575"
     CDS             3849..4679
                     /locus_tag="PKZ52_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59077.1"
                     /translation="MTYTARLLVAISSLAPLAAVADDNCRGQTPLEISYCTLVARGAT
                     GLPALYEFRKNTPDTQYLLLKRPAAKLAVALPAPPQKAKPPAVVTAPAVQPSTIPPSN
                     PTVAQAPGPLPARRKIPSAESTTGCQLANETIRCGNQQFALQWNKPKGALAADALGPG
                     NRLIFPPEPKNSAAQNDYLLSCFALYVHKMLQLGLGNTTLSFDKFAAIYATAKTEHFD
                     FAARFERMYHFLKEERKTLQPPKGFGHRPPTSLQDCQPINDNLWSCQTAEQHWIFAKI
                     "
     gene            4957..5637
                     /gene="rpiA"
                     /locus_tag="PKZ52_04580"
     CDS             4957..5637
                     /gene="rpiA"
                     /locus_tag="PKZ52_04580"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNG59078.1"
                     /translation="MLAPDILKQQVATAALEYLLPRLSPDSIIGIGTGSTANCFIDQL
                     AQHKDRFAATVASSDASEARLSNLGIKVLALNEVSHLVAYVDGADETNPRLELIKGGG
                     AALTREKIVAAAADEFICIADASKWVPVLGRFPLPVEVIPMASSYVARQISKLGGRPV
                     LRAGVVTDNGNHIIDIHDLHIHEPKTLETQLNQIAGVVTNGLFALQGADVLFLAGQDG
                     IKTFRASP"
     gene            5730..6965
                     /gene="serA"
                     /locus_tag="PKZ52_04585"
     CDS             5730..6965
                     /gene="serA"
                     /locus_tag="PKZ52_04585"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNG59079.1"
                     /translation="MSQPKTSLGKDKIKFVLLEGVHQSAVDALHKAGYTNIDYVKTAL
                     AGSELIERIKDAQFVGLRSRTQLTAEVLAQCPKLIAVGCFCIGTNQVDLHAAQERGVV
                     VFNAPYSNTRSVAELTIAQAILLLRDVPRKNALCHRGVWDKSAAGSYEIRGKTLGIIG
                     YGAIGSQVSVLAEGLGMKVVFFDVISKLPMGNAHQAASLEALLSQADIVSLHVPELDS
                     TKNLMGAAQLALMKPGAILLNASRGTVVDIDALAQAIESKHLSGAAIDVFPVEPKGND
                     EEFLTPLRAFDNVILTPHIGGSTMEAQENIGLEVADKLIKYSDNGTTTSSVNFPEVAL
                     PQQANVHRLLHVHQNVPGVLTAINQVFSQYNINIVGQYLQTKESMGYVVMDINTSYSE
                     DALEQLRNVPGTVRVRVLF"
     gene            6977..7372
                     /locus_tag="PKZ52_04590"
     CDS             6977..7372
                     /locus_tag="PKZ52_04590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNG59080.1"
                     /translation="MNYAFKLDFKVRDYECDLQGVVNNSVYQNYLEHARHEYLLANGF
                     NFAQLSAEGVNLVVVRAELLYKRPLKSGDEFWVGLTAEKISKIRGAFKQDIYRTQDGA
                     LMLSATIEWTALNHQGRPFHYSPLDALIK"
     gene            complement(7392..8114)
                     /locus_tag="PKZ52_04595"
     CDS             complement(7392..8114)
                     /locus_tag="PKZ52_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNG59081.1"
                     /translation="MTNSSVKTPQVEPDLEAGAVPTISIDCVVFGFHQGQLNVLLVKY
                     AEGFRAGGWGLPGDWILQHESLDAAAMRVLSSLTGVTDIYLEQLRAFGSVDRYPGSRV
                     VTIAFTALVRQEDYVLIAGAAASAVHWRPVSDCPDLALDHSEIFAEGLRSLRRQLRHE
                     PIGFNLLPHKFTLLQLQELYEAVLGIKLDKPNFRRKMMHMNLLIPCNEKQVSVAHRAA
                     ALYRFDETVYRELQQNGFNFSL"
     gene            complement(8302..9066)
                     /locus_tag="PKZ52_04600"
     CDS             complement(8302..9066)
                     /locus_tag="PKZ52_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNG59082.1"
                     /translation="MLYSRTQGQGEPVILLHGLFGSMENLGALARPLAEHFAVHSVDL
                     PNHGRSPHTDSTDLPAMANAVAAWMDAQGIHKAHIIGHSLGGKTAMELALSQPARISG
                     LVVIDIAPVHYPPHHNEVFKGLSQVNPAQIQQRGEAEALMLPFVPETAVRSFLLKNLV
                     KEADGFRWRMNLPVISRDYPALIAGNRAGVYTGPVLFVKGGNSDYVTSAHKSEIMARF
                     PSAQLKIVPDTGHWLHAEKPDLVSGLTLRFLQGEHT"
     gene            complement(9072..9410)
                     /locus_tag="PKZ52_04605"
     CDS             complement(9072..9410)
                     /locus_tag="PKZ52_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HNG59083.1"
                     /translation="MPVSPLYKVIFYNQNQVFEVFARAIYQSDMYGFIEVEEFVFGER
                     SGLLVDPCEEKLKAEFASVKRSYIPMHSIIRIDEVEKEGSVKVTEIKSGDKIAHFPFP
                     TLVPKPSQDQ"
     gene            9681..>10102
                     /locus_tag="PKZ52_04610"
     CDS             9681..>10102
                     /locus_tag="PKZ52_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNG59084.1"
                     /translation="MNEYDSSRMRDLLGDSHQMVATDNPEEADVLLVNTCSIREKAQE
                     KLFHQLGRWKHYKDKNPNLIIGVGGCVASQEGGAIAKRAPFVDIIFGPQTLHRLPEMI
                     DQPRSKGAVVVDISFPEIEKFDRLPQPDAEGPTAFVSI"
BASE COUNT         2574 a         2752 c         2430 g         2346 t
ORIGIN      
        1 tttccactga ttaacagtct taacagggta cgcagctccc aaaatgagtc ccgatgaatc
       61 aagccccatg tttatttaaa tatagctgca tgccaaaatt cgtggtgaag tgcaattatg
      121 aaatcttgct ctattcaata ggcattttgc tcaatctcat tatatttaag cgccaaaata
      181 atggcttttt ctagctagga acgaaaaatc tgccccgcag ccaaagaaat ggcccttggg
      241 cgaacacagg tgcaggcttg cataggaata tttaaccggg ggatttcaat cagcgtgagt
      301 tgagagcttc aacgtctctg cgccagcctg aataggtgaa tttttgcaaa cacacaagcg
      361 tgctcttgtg gctaataatc cggcggcaaa ggtaaaccag aggatgcttt agcaaaatac
      421 gtttggcggc acccaaggca atcaataacg aatgtaacta ttcagcacac tttactcctt
      481 tcttccgcgc tggcgccaaa ttcagcagga tttagccatc cggcataatc gattcgcggt
      541 gtagcagtgg tgactaccgg ttagtgcaga ccgtcaaaaa gagggacctt tttgacgagc
      601 ctacacggaa gtattcacgg cgtgtctgca ctaaccgata gtcaccactg cgggtgctct
      661 attacggggg cacagcacta aaacacacac gcgtgagttc ccagtcaggg caggaattac
      721 ccacagccac ataaaaagat gctgaatagt taaaaactaa tacgccgcct ccaactcgct
      781 ggcaaacgtt atttctttac ccgcttgatc cagttgatag gcaataaata aatccaattt
      841 gcgcgcgtca cgttcgctca tcgccaaaaa ctgcacccta aattgcgtat gctggcaggg
      901 tctgcggtag tagctcaccg atttaatcgc ggctttaaca tcaatacagg tgccgtccgt
      961 taattctaat ttcatagcaa cctgagcctt agcctgtatg gaggtatcgt gtctgccaaa
     1021 aatattcagc agcgcgccgc cggaggacag gttaatcacc cgtgcgcgca cctgctccac
     1081 catgggtatt tgcatccgca ccaacggcca ctggccgcgc gcaaaaccaa tgcgcgcccc
     1141 cgccctgcga tcacaatgaa attcccgctg gagtattttc actaacaatg ccgtgcgccc
     1201 ttcgtaaagc aaccgctcct ccacttgaac aactaactgc agcaattgct gctgcttaat
     1261 aaacgataat ttgcactggg tatggggcag cactaaatcg ataggcggcg atggaaaggg
     1321 catatcaatt aataattggt tttccactat atccacaccg agtaacgcgc tgtgaaataa
     1381 accggagcgg cccggcaact caatgcgacc caagccccgc tgctgttcat tccataaaat
     1441 taattccgac tcggcatcac tccagaccga attaacctca cacgattgtt tcacctctgc
     1501 acgagaccct tttaaccatt gacttaaacg ctcgaacact acgaaaactc ccactgaaaa
     1561 cctaagagac caactgacga tggccgcgct caggtggggc tatcgcaagg cttatgccaa
     1621 gggctgtatc aagctccagc gcccatttac tggtaggcgg cgcggatttt taccggcatg
     1681 aagcggcagg cctttgccgt accagcggca agcccttaca attgccgcct tttgcggtaa
     1741 taggtcgccc ccatgcttat gtccacgcgc gcgcgcaaat cccagtacga aaataacaag
     1801 ctgcaaaagc gcctgaggcg cttggtgggc tctgcaattg cggactacaa catgattgag
     1861 gcgggcgaca aggtgatggt gtgcctctcc ggcggaaaag actcctacgc catgttggat
     1921 atattgctgt cactgcaacg ggcggcgccg gttagttttg aaattgtggc ggtgaatatg
     1981 gatcagaagc agcccggttt tcctgaggag gtgctgccaa attatttaag cgccttgggg
     2041 gtgccctttc acatcattga aaaagacacc tactcagttg ttaaatccat tattccggaa
     2101 ggcaaaacca cctgtggttt atgctcccgc ctgcgccgcg gcaccctgta tgggtttgcc
     2161 gaatccatcg gcgccaccaa aattgccctg ggccaccaca aagacgacat agtggaaacc
     2221 ctgtttttaa atttgttttt tgccggccgc ctcaaagcca tgccacctaa attattggcc
     2281 gatgacggcc gcaatattat aatccggcca ctggcgtatt gccgggaaga agatttaatt
     2341 gaatttgcag cggtgaaaga attccccatt atcccctgca acctgtgcgg ctcccaggag
     2401 aatctgcagc ggcaatccat aaaaaccatg ctggccagct gggaaaaaca atacccaggc
     2461 cgcattgata atattttcgg cgccctgcaa aaggtgtcac cttcgcaatt ggccgataat
     2521 aatttatttg attttattaa tttgcattta aaccgcagcg gggaactcac cgaaagtgac
     2581 gacgcctgca cactcacaaa taataccgcc acgcctgctg cgcaatctgc cgatgtgcag
     2641 tattttcgcg catttgacct tgaataaccg cctgtaaaga ttgtacccat gtctaaaaaa
     2701 attatagtgc ccttagttac ccttttactt gctggtgcag gcaccctgag cttaaaaccc
     2761 tgcattatcc ccggccccat atgcgaagcc catatggaaa aagtaggcgc ccaaaaggat
     2821 aaggacaatc cttattatct gtcctttacc acaccctggc ttgcccagcg ggcaaacata
     2881 ctctaccccg cctatgccat ggatcgcggc cttacgggtg tcaccgaatt agagctgcgc
     2941 attgatagcg acggcaaact gctggaaaaa tctattagcg aatcctctgg ccaccctatt
     3001 ctcgataagg ctgccttagc cgccgccgat acatttgtat ttaacgtcgc agggtttggc
     3061 aatgtgttat ttccctactc gaaggaatta aaaatacgct tccgattgga tgacgggccg
     3121 cactaaaact agcaattgcc gcagttgcgg ttcgaggcga tagagaaggg ggaattacca
     3181 gcgggtaaaa aaccatttga cgagaattat tgttatggct cgttacaaat ggcactgcat
     3241 acctaaatta actggcaggc ggttacgcgg ccgctttgta gcggcttaaa atatcaataa
     3301 aatccgggtt atggttcacc acataatcaa taaattccct cgcgccccgc tgggcttcct
     3361 cctgattatc aaacggccct atatcaattc cctcgcgggt gcggtagtac caataatcat
     3421 gttgctgaaa aaaacggttg tgcctggccg gcacagaacc cgattcgcct gaacgattca
     3481 taatgaaata cccttgggag tccaaatatt aaatataccg gccggacggc aaactccttg
     3541 attcctttcg atgacagcac cttttattgc attcacgcca tttgccgtga aaacccaagg
     3601 ccgcgcattt accgattaat ccctgcagtt gctagcacat taaccatcat tgagtcagct
     3661 aagccgctgc tcaatatcaa tgagtttagc ctgtgatcaa ttcttgtgta gcgaaaagtt
     3721 agcttatgta attattacca tggcgtttgg ttatagctaa gcatgctcga ctgaacccaa
     3781 tatctggcac ctttgcggcc tttactctgc gcgctaccat agccctccgc tttattgact
     3841 cactcccgat gacctacacc gcacgcctgt tagttgccat ttcctcgctc gcaccattgg
     3901 ccgccgtggc cgatgacaac tgtcgagggc aaacaccctt agagataagt tattgcacgc
     3961 tggtagcacg cggcgctaca gggctgcccg cactgtatga atttcgcaaa aatacgccgg
     4021 atacacagta tttattatta aaacgcccag cggcaaaatt ggccgttgca ctgcctgcgc
     4081 cccctcaaaa agccaaaccc ccggcagttg taacagcgcc agcggttcaa ccgagcacca
     4141 tcccaccgtc aaacccaact gttgcacaag cgccggggcc gctcccggcg cgccgtaaaa
     4201 ttcccagcgc tgaatccaca accggctgcc agctagccaa tgaaaccatt cgttgcggta
     4261 accagcagtt tgccctgcag tggaataaac ctaaaggcgc acttgcagcg gatgcgttgg
     4321 ggccgggcaa tcgcttaatt tttccccctg aacctaaaaa ctccgcagca caaaacgatt
     4381 atctgctcag ctgctttgcc ctttatgttc acaaaatgct gcagctaggg cttggcaata
     4441 ccaccttaag tttcgataag tttgcggcca tttatgcgac agccaaaacc gaacactttg
     4501 attttgccgc ccgctttgag cgcatgtacc acttcttaaa agaagagcgc aaaaccctgc
     4561 aaccacccaa aggcttcggc caccgccccc ctaccagcct gcaggattgc caacccatca
     4621 acgacaacct gtggagttgc caaacagccg agcagcattg gattttcgca aaaatctaat
     4681 ttaaacctaa aaaccgcagt tgaactcgta gcgagggcgc ctaaggcgcc gaatatgtgc
     4741 gcttttttat caaaatttgg cgaaagcagt agtcatctct acggtgagcc aaattttgat
     4801 aaaaaagcgt gcagattcaa gcattaggca gccgtactcg agttcaactg cggtttttag
     4861 gttaaagcca gccggttttt aacgctgaag tttccaaccc gcccgcctgt ggtttgggta
     4921 aaatgcgcac ctttcttaat ggttaatagg cccatcatgc tcgcgccgga cattctcaaa
     4981 caacaggtag ccaccgccgc cctagagtac cttttacctc ggctgagccc ggatagcatt
     5041 atcggcatag gcacaggttc caccgccaat tgttttatcg atcaattggc gcagcacaaa
     5101 gaccgcttcg ccgccaccgt cgccagctca gatgccagtg aagcaaggct tagcaacctg
     5161 ggcattaagg tgttggccct caatgaggtc agccatttag ttgcctatgt ggacggcgcc
     5221 gatgagacca acccgcgttt agagttaatt aaggggggcg gtgccgccct aacccgggaa
     5281 aaaattgtcg ccgccgccgc ggatgaattt atctgtatcg ccgacgcctc taaatgggta
     5341 ccggtattgg ggcgctttcc gctgccggtg gaagtaatcc ctatggccag tagttatgtg
     5401 gcccggcaaa taagcaaact cggcggcagg cccgttttgc gcgccggtgt ggttactgac
     5461 aatggcaacc acatcatcga cattcacgat ttacatatcc acgagcctaa aaccttagaa
     5521 acgcagctca accaaattgc cggggtggtt accaatggtt tgtttgccct gcaaggcgcg
     5581 gatgtgttgt ttttagcagg ccaagacggc attaaaacct tccgcgccag cccctaaacc
     5641 accggcgctg cggcgcggtt actcataagt tggcggccct gcctgtggta gaatcgccgc
     5701 ctttttgttg tccaccactg gaagtcccca tgagccaacc taaaacctcc ctcggcaaag
     5761 acaaaattaa attcgtactg ctagaaggcg tacaccagtc ggcggtggac gcactgcaca
     5821 aggccggtta caccaatatc gattatgtaa aaacggctct ggcgggctct gagttaatcg
     5881 agcgcattaa ggatgcccag tttgttggcc tgcgctcgcg cacccagctc actgcagagg
     5941 tgttggcgca gtgtcctaaa ttaattgccg tgggctgttt ttgcattggc accaatcagg
     6001 ttgaccttca cgccgcccaa gagcgcggcg tggtggtatt taacgcgccc tactccaaca
     6061 cccgcagcgt tgccgaactc accatcgccc aggccatatt attgctgcgg gatgtgccgc
     6121 gtaaaaacgc cctctgccat cgcggtgtct gggataaatc ggcggcgggc tcctacgaaa
     6181 ttcgcggcaa aaccctcggc attatcggct acggcgccat tggctcccag gtgagcgttt
     6241 tggctgaagg cctgggcatg aaggtagtgt tctttgatgt gatcagcaag ttgcccatgg
     6301 gtaatgccca tcaggccgca agccttgaag cacttttaag tcaagcggat atcgtcagcc
     6361 tgcacgtgcc cgaactcgat agcaccaaaa atttaatggg cgctgcccaa ttggcgctga
     6421 tgaaacccgg cgcaatattg cttaacgcca gccggggcac ggtggtggat attgacgcgc
     6481 tcgcccaagc catcgaaagc aaacacttaa gcggcgcggc cattgatgta ttcccagtgg
     6541 aacccaaggg caatgacgaa gaatttttaa cgccgctgcg ggcctttgac aatgtgattt
     6601 taacgcccca catcggcggc tccaccatgg aagcacagga gaatataggc cttgaagtgg
     6661 cggataaact aattaaatac agcgataacg gcaccaccac cagctcggtg aatttcccag
     6721 aagttgcgct gccccagcag gccaatgtgc accgtttatt acacgtccac caaaatgtgc
     6781 ccggtgtatt aaccgccatt aaccaggtat tttcccaata caacattaat atcgtcggcc
     6841 aatacctaca aaccaaagag agcatgggtt atgtggtgat ggatattaat accagctaca
     6901 gcgaagacgc cctcgaacaa ttgcgcaatg tgcccggcac tgtgcgcgtg cgcgttttat
     6961 tttaagagcg agcgcaatga actacgcttt taagttggat tttaaagtgc gcgattacga
     7021 atgcgacttg caaggcgtag tcaacaacag tgtgtatcaa aattatttag aacacgcccg
     7081 ccatgaatat ctgctcgcaa acggctttaa ttttgcgcag ctttccgcag agggcgtgaa
     7141 tttagtggtg gtgcgagcgg agttgctcta caaacgccca cttaaaagcg gcgatgaatt
     7201 ttgggtgggg ctaacagctg agaaaatttc gaaaattcgc ggtgcgttta agcaggatat
     7261 ttaccgcaca caggatggtg cactcatgtt aagcgccact attgaatgga cagccctgaa
     7321 tcaccagggc cgcccctttc attattcgcc gttagatgca ctcattaaat agcagcacat
     7381 ttaagagcaa tttataagga gaagttaaaa ccgttttgct gtaattcgcg gtaaacggtc
     7441 tcgtcaaacc gatacaaagc cgccgcccgg tgcgccaccg atacctgttt ttcattgcaa
     7501 ggaattaaca ggttcatgtg catcattttg cggcggaaat tgggtttatc cagcttgatg
     7561 cccaatacag cttcatacaa ttcctgcagc tgtaataggg taaatttatg cggtaacagg
     7621 ttaaagccaa tcggttcatg gcgcagctgc cggcgcaaac tgcgcaaacc ttccgcaaaa
     7681 atttcactgt gatccagtgc caagtcaggg caatcactca ccggccgcca atgcaccgca
     7741 ctcgccgccg cacccgctat taatacatag tcctcttgcc gcaccaaagc ggtaaatgca
     7801 atggtcacta cccggctgcc aggataacga tccacgctgc cgaaggccct taattgttcc
     7861 aggtaaatat cggtaacgcc ggtaaggctg ctaagcaccc gcatggccgc tgcatcgagg
     7921 gattcgtgct gtaatatcca gtcgccaggc aaaccccagc caccggcacg aaagccttcc
     7981 gcgtatttaa ccagtaatac attcagctgg ccctggtgaa agccaaacac cacacagtcg
     8041 atggaaatag tcggcaccgc gcccgcttca agatccggtt ctacctgggg agtcttaacg
     8101 ctagagtttg tcaaagtatg tctcctggct gcagggaatt cggctgtata ttagatgata
     8161 ttgcagttta aatctgataa aagtattaca tttatgccca ttggtaaaac cgtttggcgc
     8221 gccgcagcag ccagcgccgg gtaaccagct gcaattgaac ttgtgacctg tgtgcaattg
     8281 ctcgggcaga cgcccctacc gctaggtgtg ctcgccttgc aaaaaccgca gtgttaatcc
     8341 gctaactaaa tcgggttttt cagcgtgtaa ccaatggcca gtatcgggga caattttaag
     8401 ctgcgccgac gggaagcgcg ccattatttc gctcttgtgg gcgctagtaa catagtcgga
     8461 atttccgcct ttcacaaata acaccgggcc tgtatacaca ccggcccggt tgccggcgat
     8521 gagcgcgggg taatcccggc tgatcaccgg caggttcata cgccaacgaa acccgtccgc
     8581 ttcttttacc aagttcttca ataaaaagct gcgcacggcg gtttccggca caaacggcaa
     8641 cataagcgct tccgcctcgc cgcgctgctg aatctgggcg gggttcacct ggcttaagcc
     8701 tttgaagact tcgttgtgat gggggggata gtgcactggg gctatgtcaa tcaccaccag
     8761 gccactgatg cgcgcaggct ggcttagcgc caattccatg gccgttttac cgccgaggga
     8821 atggccgatg atatgggcct tgtgaatgcc ctgggcgtcc atccaagccg ccaccgcatt
     8881 ggccatggca ggcagatcgg tactgtcggt gtgggggctg cggccgtggt tgggcaaatc
     8941 cacactgtgc acggcaaagt gctctgccaa gggccgggcc agagcaccta aattttccat
     9001 ggagccaaac aggccgtgca gcaagataac cggctcgccc tgcccttggg tgcgcgaata
     9061 gagcaccgga attattgatc ctggctgggt ttgggcacca gcgtagggaa cggaaagtgg
     9121 gcgattttat cgccggattt aatctcggtt acctttactg agccctcttt ttccacttcg
     9181 tcgatgcgaa taatcgagtg catggggata taagagcgtt taaccgaggc gaactccgct
     9241 ttgagttttt cttcacaagg gtccaccaac aggccgctgc gctcgccaaa tacaaactcc
     9301 tccacctcga taaaaccgta catatcgctt tggtaaatgg cgcgggcaaa tacctcaaat
     9361 acttggtttt ggttgtaaaa aataactttg tagagcggag agacgggcat aattggaata
     9421 ctcattggct tattgccttt aacaagggcg ccttcaaaaa agctagccgc ccattgtggg
     9481 ggctgcgggc aaaattacaa gcgcgaaaaa ccatgggaaa gcgcaaggca catcagtata
     9541 atgccgcgcg caatttttaa gcacctatca acaggccacc tatgtccgac cttccatctc
     9601 acgccctgtc gccccttaac accaccggct cacaagcccc tggtgtaaag aagttattta
     9661 tcgaaaccca cggctgccag atgaatgaat acgattcgtc gcgcatgcgg gatctgctcg
     9721 gggacagcca tcagatggtc gccacagaca acccagaaga ggccgatgtt cttttagtca
     9781 acacctgctc tattcgggaa aaagcccagg aaaaactctt tcatcaattg ggccgctgga
     9841 aacactacaa agataaaaac cccaacctga ttatcggcgt gggcggctgt gtggccagcc
     9901 aagaaggcgg cgccatcgcc aaacgcgcgc cctttgtgga tattattttt ggcccccaaa
     9961 ccctgcaccg cctgcctgag atgattgacc aaccccgcag caaaggtgct gtggtggtgg
    10021 atatttcctt cccggaaatt gaaaaatttg accgcctgcc tcagccggat gccgaaggcc
    10081 ccaccgcttt tgtctccatc at
//
LOCUS       DAOQGS010000080        10017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3136_length_10017_cov_5.126882, whole genome shotgun sequence.
ACCESSION   DAOQGS010000080 DAOQGS010000000
VERSION     DAOQGS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3136_length_10017_cov_5.126882"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1928)
                     /locus_tag="PKZ52_04615"
     CDS             complement(327..1928)
                     /locus_tag="PKZ52_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNG59085.1"
                     /translation="MNIRRLASLHILLVTHLLLIACSGQPGNSSSGSSSSSTSSTSSS
                     GRPSSSSSSSSSSSGLAVAPRCASRESNAFNSFAQIVPGKVEAENFNPKQNVDTTEGN
                     LGKVYRLDTNVDIKEIAGGYALGWMDKNEFISFTVYVQTAGAYDVYLRAGATGAGRTL
                     RLEQCYDLLLSRIDIPTVEAPGQFKIFKAGTVQLTAGSQMISVRTGNLEGIELDWLHL
                     GPYAGPIDPVSTSSSSSSSSSSNSSTSSGTIAHKFFVGNTTTNWQVRSDFAQYWQQIT
                     PENEGTWASVEGTRDTYNWQRLDTIYTYAKEHNILSKHRAFLWGAGLPAWLTNLNSAD
                     AALEIEEWISEFCHRYPDIELIEVVNEPSAGHAPTAIFQRALGSDWITKSFQLARKYC
                     PNSVLMLNDYNLERYSNADFIALVMPAVKSGYVDAIGLEAHNLEGFAAAELSASLDDL
                     WNKLQLPMYITEYDSKGTDAEQLKNYQAQFPVFYNHPHIKGITLWGYVFGKTWVTGTG
                     LIQDDGTPRPAMTWLMDYIKTHPKN"
     gene            2225..2440
                     /locus_tag="PKZ52_04620"
     CDS             2225..2440
                     /locus_tag="PKZ52_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59086.1"
                     /translation="MGSISALFKADGAQAGGNYCVSGGLRPIQRGLVRIARIIRVRVL
                     ENSADISKRWLHVLHFALFALFARSCF"
     gene            2876..3208
                     /locus_tag="PKZ52_04625"
     CDS             2876..3208
                     /locus_tag="PKZ52_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG59087.1"
                     /translation="MATPRAYSQYAQDAALLLGEHIKLARKRHKWSELALAERAGISR
                     ATLQKIESGDMGCAIGLYFEAAALVGINLFEQDSQPLTKAIEQVRDKIALLPKRIKSK
                     TRVIHDDF"
     gene            3198..4466
                     /locus_tag="PKZ52_04630"
     CDS             3198..4466
                     /locus_tag="PKZ52_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNG59088.1"
                     /translation="MTSKNRNKTHEAFVWVWLANETKPIVAGRLEAINRTVLFNYGKS
                     YIERINDTAPAMSLYEPELPLRAGELPLLDGLSMPGCIRDAAPDAWGRRVIINKKLGL
                     KGKNTNTAELDELTYLLESGSDRIGALDFQRSPTEYVPRAAANVPLSELLESAERVEK
                     GFPLTPELDLALYHGSSIGGARPKALIEDQGNKYVAKFSSQDDLYNVVKAEFIAMRLA
                     KLAGLTVAPVQLVRAANKDVLLIERFDREKTAHGWTRKTMVSALTLFGLDDMMARYAS
                     YEKLAEIIRHRFTHPKETLHELFSRLVFNILCGNTDDHARNHAAFWDGKYLSLTPAYD
                     ICPQGRAGNEASQAMLISGNKNLSQLKNCLETAHNFFLSEQAARRIFTRQKDIIENNW
                     AVVCDEAELSAVDRALLWQRQFLNPFSVEE"
     gene            4810..5247
                     /locus_tag="PKZ52_04635"
     CDS             4810..5247
                     /locus_tag="PKZ52_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59089.1"
                     /translation="MIRLIFIFASVLLTASCSKSGSASSKAAETPMTFEEAFKFVDKQ
                     IAEKDPTIIEYLGRRMAFENSISLDFKGNCYAIPGKTVTLVSRINQAGVIDLVIADAE
                     NERSECFKNTYLNTRFDSPPVSPIYRFNIMLGEDGLPERFKNL"
     gene            complement(5425..8157)
                     /locus_tag="PKZ52_04640"
     CDS             complement(5425..8157)
                     /locus_tag="PKZ52_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG59090.1"
                     /translation="MTLNSKNVQLPQYLTRFSLVYCAYLVTAWLGLSVPFVSDTVTLF
                     WLPSGISVAALFRWGWKIIPAIFLAAFTINVVKGADIIAGSIIACGNSLGPLVAASLL
                     KQWRCNLLRLKKYTALKLIAAAILGTLISALIGAGTLAFTQVIPSNKIAITALVWWLG
                     DSLGVVLCAPLLISLSHKSLHAVVRQPRATIVFCVVALATALICFPLNHLINGSPLPI
                     VFITFVCVAWAALTLGLIGTASCTIVFSFIAIWSSTEQLGPFVFADQQMSYWTIWIYT
                     IAMTIFGMMITSAHGEIIDTSEQLHLANTELLAQQQELQAINNALSIAAADAINAQDF
                     IASLLKSLGQVLGADYLLVSLVDATKTQATTHTFYCDGEIKPNFTYPLHHTPCEDSLA
                     QSFCFIPQDVQSLYPEDEMLVQEGIESYIGVVIQNIQGTSIGILTALSRKKIEFKPQF
                     QSLMKIYSERIAGEIRRADDQEKIFNLAFYDPLTLLPNRRLLQERLKLITALSTRTGQ
                     YGAILFIDIDHFKFLNDTLGHHVGDQLLTLVAQRISSIIRSTDLAARLGGDEFVVIFD
                     NLGTTGELAALEAKKRAEELHALISQPYPLQNTVHHCTISLGVNMFLGSQNSTDDLLR
                     HADLAMYHAKDSGRNAIRFFDPHMQLQMELRSSIESDLRAALKSETQLVPYYQIQIDS
                     AGAAIGAELLLRWLHPTKGLIAPADFIPIAEQAGIITQIGHKVLQMACSQLVHWQAKA
                     QFSALKLAVNVSPIQLNQNNFVADTWDLIKASGIDPRLLTLELTESSLTNNIDASIAK
                     MRALQEQAISFAMDDFGVGYSCLSHLKRLPLHQLKIDRSFIRDIASDPNDRVIVRTII
                     AMAKNLELDVLAEGVETLEQKHYLIEHGCNTFQGYYFGRPVPIHEFEAMRAQFG"
     gene            9109..9966
                     /locus_tag="PKZ52_04645"
     CDS             9109..9966
                     /locus_tag="PKZ52_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59091.1"
                     /translation="MMDNDDIWITYSEKSNSSIVVGTQIFVSLMLLVAIAKIGALPSA
                     KGWALVISLPLIWLFLLNLSGTQYTAVNFIVHNIVEDRTLFGSVCTRLISFSDITFFA
                     YDPVNGHNRLKIILRSGKTHFFNFGNILSPRYQRLISAIDASNPNPYRSAVQLDDASV
                     ACLQIAPIHFTPDQITLDVKNQKIIVGGKPETVPVEIRFSDISNVIVLIDVDSDPADE
                     TKTFNRYCLCAILNSFEKIELTDYETDRQGDEVRDKMWAARLHVNAYLERAHGKRVTR
                     SDFWDCVGP"
BASE COUNT         2678 a         2295 c         2381 g         2663 t
ORIGIN      
        1 gttttaattt cccgtgcaat ggggatacct gcaagaaata atgctactgc ttttttgaca
       61 cggtaccttg cacccaggga atctatgtgc agattccatc gctcccaaat caccgcaagc
      121 ggctaattat ggccgttgcc gttgccgttg ccgttggtat gggaggatgg tatcgacctt
      181 acggattagc cgtgtagtgc gggtcatttg acccgcgcgg tttcgtcctt tgccattaag
      241 gaaataattt tactttttaa agtgtgtttg aatttgaatt aaatctgcgg gttacgcctt
      301 cggctaaccc gcactacgcg tgcccactaa tttttagggt gggttttaat gtaatccatt
      361 agccatgtca tagctgggcg cggtgtgccg tcgtcttgaa ttaatcccgt acccgtcacc
      421 caagtcttac cgaacacata accccatagg gtgatacctt taatatgcgg gtggttgtaa
      481 aacacgggga attgcgcctg ataattttta agctgctctg catcagtacc tttactatcg
      541 tattcggtaa tgtacatagg caactgcaat ttattccaaa gatcatccaa tgatgcggat
      601 aattccgctg ctgcaaaccc ttccaagtta tgagcttcca ggccgattgc atccacatag
      661 ccacttttta ctgccggcat taccagggca ataaaatcag cattggaata gcgctccaga
      721 ttataatcgt tgagcattaa cacggaattt gggcaatatt tgcgggccaa ttgaaatgat
      781 ttagtgatcc aatcactgcc cagcgcccgt tggaatattg ccgtgggagc atggccggca
      841 gagggttcat tcactacctc aatgagctca atatcggggt agcggtgaca aaattcgctg
      901 atccattctt caatttccag cgcggcatcg gcagaattta aattggttaa ccatgcaggc
      961 aaacctgcgc cccagagaaa agcacggtgt ttagataaaa tattatgctc ttttgcatat
     1021 gtatagattg tatctaaacg ctgccaatta taggtgtcgc gagtgccctc tactgatgcc
     1081 caggtacctt cattttcagg ggtaatttgc tgccaatact gggcaaaatc actgcgcacc
     1141 tgccagttgg tagtagtatt accgacaaaa aatttatgcg cgatagtgcc actgctggta
     1201 ctgctatttg aactgctaga agaactggat gaactgctgg tgctaacagg atcaatcggc
     1261 ccggcatagg gccccaggtg cagccaatcc aattcgatgc cttctaggtt gcccgttcgc
     1321 actgaaatca tttgtgagcc tgccgtcagt tgtactgtgc ccgccttaaa gattttaaat
     1381 tgccctgggg cttctactgt tgggatatca atacgactga gcagtaggtc gtaacattgc
     1441 tctaatctga gcgtcctgcc tgcgcctgta gcacctgcgc gcaaataaac atcgtaggcg
     1501 cctgccgtct gcacataaac ggtaaaactt atgaattcat ttttatccat ccaacccaag
     1561 gcataaccgc cggcaatttc tttaatatca acattggtat ctagccggta taccttaccc
     1621 aggttgcctt cggtggtgtc aacattctgc ttggggttga aattttcagc ttccaccttg
     1681 cccggaacaa tctgcgcaaa actattaaat gcattgctct cccgggaggc acagcggggc
     1741 gcaactgcca gccccgagct actgctagac gatgaactac tgcttgaagg gcgtccgcta
     1801 ctggatgtag atgaagtact actggaggac gaaccggagg aactattgcc cggctgccca
     1861 gaacaagcga tcagtaataa atgggtaacc aataaaatgt ggagggacgc taaccggcgt
     1921 atgttcatag gtgaaccttc ctaactgttt aatttattgg gtgttttatt atcgtgttcg
     1981 gcacctatag gcgtactaag tcggcaaata caggctgcag tgcgcgaacc ctagcatagg
     2041 ccatgggaga tttttttccg ttgactaatg cacagagttt gctatttgta taacaaataa
     2101 ggaggggtgc ctggccatgg ctggcacctt gctgggggca tctaacaccc ggagaatttt
     2161 tgcgaaaatt atatgaatca agaaccaatg gatttaatgc caggagaggg acggcccata
     2221 cgctatgggt tctatctctg ctttgtttaa agccgatggc gcgcaggccg gtggaaatta
     2281 ctgcgtatcg ggtggcctga ggcctattca aaggggcctg gtgcgcatag ccaggattat
     2341 tcgtgtcaga gtccttgaaa actctgctga tattagcaag cgctggcttc atgtcttgca
     2401 ttttgccctt tttgcattat ttgcacgcag ctgtttttaa ggcagattat ttagggttgg
     2461 cccctgcgtt aaatacgaaa taggcacaac taataatttt caccaaacgg caatttgctt
     2521 cgctaaagcg gtgatagagc actgtatcca tcggctttta tggcgccgca gcctttttat
     2581 gggttcggct atgtgtagat agatgttcgt ccaaatgaaa gtattggttc ggctatattt
     2641 agcgggaacc caatacctaa tacccaatac tggcgagcgt acgaacccag acatcccgag
     2701 cctagcatta ccatcggatg actattaaag cagtcattat gcgcaataat ggctattatt
     2761 ttagccattt ttattgatca attaaccatc atatgctaga ttgaatagca tactggtcat
     2821 agatacattt aatgaccata aagatagtca tttttttaga taagtgagcc tcacagtggc
     2881 aacaccaaga gcatactctc aatacgcgca ggatgccgcc cttttactgg gtgagcatat
     2941 caagcttgcg cgcaaacgcc ataagtggtc tgaactcgcg cttgcagagc gagcagggat
     3001 ttcgcgcgcc accttacaaa aaattgaaag cggtgatatg ggctgcgcaa tcggcctgta
     3061 tttcgaggct gccgcacttg tcggcattaa cctgtttgag caggattcac agcctttgac
     3121 gaaagccata gaacaggtca gagataagat cgccctcctc cccaagcgca tcaaatcaaa
     3181 aactagggtg atacacgatg acttctaaaa acaggaacaa aacccatgag gcctttgtat
     3241 gggtatggct ggcgaatgaa accaagccaa ttgttgcggg aaggctggaa gccataaatc
     3301 gcacagtgtt gtttaactat ggtaagagct atattgaacg cattaatgac accgcgccag
     3361 caatgtcact ttatgaacct gaattaccgc ttcgggccgg tgagctgcct ttgcttgatg
     3421 gtttgtccat gccaggctgc attcgtgatg ccgcacccga cgcgtggggc cgcagggtca
     3481 tcatcaataa aaagctggga ttaaagggta aaaataccaa cacagcagaa ttggatgaac
     3541 tgacgtatct gctggaatca ggctctgatc gtatcggcgc attagatttt cagcgctcac
     3601 ccacggaata tgtaccccgc gcagcagcga atgttccgct gagtgaatta ctagaatccg
     3661 cagagcgtgt agaaaaaggt tttccattaa cgccagaact tgatcttgcg ctctaccacg
     3721 gcagctcaat cggcggcgcg agacccaaag ccctgataga agatcagggc aacaaatatg
     3781 tcgcaaaatt ttcttcacag gatgatttat acaatgtagt taaagccgaa tttattgcta
     3841 tgcgcttggc aaaattggcg gggcttacgg tcgcccccgt tcaactggta cgcgccgcaa
     3901 ataaagatgt gttgttaatc gagcgatttg accgtgaaaa aacagcgcat ggctggacac
     3961 gtaaaaccat ggtttctgcg ctcaccctct ttgggctgga tgacatgatg gcacgctacg
     4021 caagttatga aaaacttgca gaaattatca ggcatcgttt tacacatccg aaagaaacgc
     4081 tgcatgaact tttttcgcgt cttgttttta atatcttgtg cggcaatacc gatgaccatg
     4141 cacgaaacca tgcggcattt tgggacggca agtatctcag ccttacaccc gcttacgata
     4201 tatgccccca agggcgcgca ggcaatgaag catcacaagc catgctcata tcaggcaata
     4261 aaaacctgag ccagcttaaa aactgccttg aaacggcgca taattttttt ctatccgaac
     4321 aagccgcacg ccgcattttt acaagacaaa aagatattat tgaaaataac tgggcggtgg
     4381 tttgtgatga agccgagctt agcgcggtag acagagcctt actttggcag cgtcagtttc
     4441 taaatccatt ttccgtggaa gagtaaacag gctcgtcagc agctatgtag agcaggtctt
     4501 tgacccgcac ggctttgtgc atggttattg aggaaataat ttcacttgtt tggcgtgtgc
     4561 ttaaatttta attaaacatg tgggttacgc cttcggctaa cccgcgccac gtactaatta
     4621 taggccgaaa cagatgcagc aagctggctg gcagagctag tgtaaatttt aatcaccaag
     4681 aaatcacaat tgtgttgcaa taaacctaac tgcggcagat attttgctgg caacaaatat
     4741 gtattatgct gggtttcgct atgctccacc caaccaactt tccatattta attgcggtaa
     4801 cattttcaga tgattcgatt aatttttata tttgcctcag ttttacttac agcgtcttgc
     4861 tccaaatctg gcagcgcctc gtctaaagct gccgaaaccc caatgacctt tgaagaggca
     4921 tttaaatttg tcgacaaaca aattgcagaa aaagacccga ctataatcga atatttgggg
     4981 aggcgcatgg cgttcgaaaa tagcatcagc ttggacttca aagggaattg ttatgctatt
     5041 ccaggcaaaa cggtcacctt agtttcgcgc atcaaccaag ctggcgttat agatttggtt
     5101 atcgccgatg cagagaatga acgtagtgaa tgtttcaaga atacctattt aaatacacgg
     5161 tttgatagcc caccggtttc cccaatttac cgctttaata ttatgctagg cgaagacggc
     5221 ttgcccgagc ggtttaagaa tctttaacag aatcatcggg cacaactcaa tgggtcaacg
     5281 gcgttaacag gacttgcgga aatgctcgtt aaaattttga gaaaatttag cgttttaact
     5341 tcaagcaata agcgctggcc cgtagtttgt tcaacgctta ataatccggt gacatctagt
     5401 cacgccttaa taaataagcc gccatcaacc aaattgcgcc cgcatagctt caaactcatg
     5461 aataggcacg gggcggccga aataatagcc ttgaaatgtg ttgcagccat gttcaattaa
     5521 ataatgtttc tgctccagtg tttccacgcc ttctgctaat acatccagct cgaggttttt
     5581 agccatggca ataatagtgc gcactatgac tctatcattg gggtcggagg ctatatcgcg
     5641 gataaacgag cggtcaattt taagctggtg cagtggcagg cgttttaaat gggataggca
     5701 cgaataaccc acgccaaaat catccatggc aaagcttata gcctgctctt gcagggcgcg
     5761 cattttggca atactggcat cgatattatt ggttaaagag ctttcggtaa gctccaaggt
     5821 gagcaggcgc gggtcgatgc ccgaggcttt aattaaatcc caggtatccg ccacaaaatt
     5881 gttttggttt aactgaatgg ggcttacatt aactgccagt tttaaagcgc taaattgtgc
     5941 cttcgcctgc cagtgcacta actgactaca cgccatctgt aataccttgt ggccgatctg
     6001 ggtgattatt cccgcctgct cggcaatggg aataaaatcc gcaggcgcaa taagcccttt
     6061 agtggggtgc agccaacgca gtaacagctc tgcaccaatg gcggcaccgg cgctatcaat
     6121 ttgaatttga taataaggca ccagttgtgt ttcgcttttt aaggctgcgc gcaaatcact
     6181 ttcaatggat gagcgcaact ccatttgtaa ctgcatgtgc gggtcgaaaa agcgaatggc
     6241 attgcgcccg ctgtctttgg cgtggtacat ggcaagatcc gcgtggcgca acaaatcatc
     6301 ggtgctgttt tggctgccca gaaacatatt aacacccagg ctgatggtgc aatggtgcac
     6361 ggtgttttgc agcgggtagg gctggctgat caaagcgtgt aattcttcgg cgcgtttttt
     6421 cgcttctaag gcggcaagtt ctcctgttgt acctaaattg tcaaaaatga ccacgaattc
     6481 gtcgccgcct aggcgggcgg ccaaatcagt gctgcgaata atactgctaa ttctttgcgc
     6541 caccagcgtc aataattgat cgcccacatg atggcccagg gtatcgttga gaaatttaaa
     6601 gtgatctata tcgataaata aaatcgcgcc gtattggccg gttcgggtgc tcagagcggt
     6661 aatgagtttc agccgctcct gcagcaggcg ccggttgggc aacagcgtga gcggatcata
     6721 aaaagctagg ttgaatatct tttcttggtc atccgcgcgt ctaatttccc cggcaatacg
     6781 ttcggaatat attttcatca aggattgaaa ctgcggttta aactcaattt ttttgcggct
     6841 caatgctgta agtatgccta tagaagtgcc ctgtatattt tgtattacca cccctatata
     6901 actttctata ccttcttgca ccagcatttc atcttcaggg tataggctct gcacatcctg
     6961 tgggataaaa caaaaagatt gcgccagcga gtcttcacag ggtgtgtgat gtagcgggta
     7021 ggtaaaattg ggttttattt caccgtcgca gtaaaacgta tgggttgtcg cttgggtttt
     7081 cgttgcgtcc accaagctga ccagtaaata atccgcgcct aatacctggc ctaaagattt
     7141 taatagtgaa gcaataaaat cctgcgcgtt gattgcatct gcggcggcta tggagagcgc
     7201 attattaatt gcttgcagct cctgttgttg cgccaataat tccgtattgg ctaggtgtaa
     7261 ttgttcgctg gtgtcgataa tttccccgtg ggcagaggta atcatcatgc cgaaaatagt
     7321 catggcgatg gtgtagatcc aaatagtcca ataactcatt tgctgatcgg caaatacaaa
     7381 tgggcccaac tgttctgtag acgaccaaat ggcaataaaa ctaaacacaa tggtacaact
     7441 ggcggtgcca ataaggccta aggttaatgc cgcccaagcc acgcagacga aggttataaa
     7501 tacaatcggc agtgggctgc cgttgataag gtgattcaag ggaaaacaaa tgagcgccgt
     7561 cgccagtgcc accacacaaa atactatcgt agcgcgcggc tgccgcacta ccgcgtgcag
     7621 cgatttgtgt gacaggctaa ttaataacgg ggcgcataat acaacgccta aactgtcgcc
     7681 aagccaccaa actagggcag taattgctat tttattgctt ggtattacct gggtaaatgc
     7741 cagcgtaccg gcgccaatga gcgccgaaat aagcgtgccc aatatggcgg ctgcgattaa
     7801 tttaagtgcc gtatattttt tcagacgcaa aaggttgcag cgccattgct tgagtaaaga
     7861 agcagcaact aacggcccca gactattgcc acaggcaatg atagaccctg caataatatc
     7921 cgcgcctttg acgacgttga tggtaaaagc ggctaaaaaa attgccggaa ttattttcca
     7981 accccagcga aatagcgcgg ccacgctaat cccactcggc aaccagaaca gcgttactgt
     8041 atcgctgaca aacggcacgc taaggcccag ccacgcggtg actaagtacg cgcaatagac
     8101 cagactgaag cgggttagat attgaggcaa ttgaacgttt ttagagttta atgtcattgg
     8161 cctttacgtg gcgattgaga ttggttgatt ggaatttaag ggttgcggtg aagggtggaa
     8221 attccatttt tgttttgccc ctaagcaaat gcaaacatta aaatttagcg atgagaacaa
     8281 ggtatttatc ccacgggtgc agagggctgc agtggtcata gcacctaaat ccatttaggc
     8341 gtgcgtcgtt ctacctccta ccgcgatcat cttgcaaagc agccctaaat cccttgccat
     8401 ggtgtgcaaa cagaaacctc agttaagtgt tcggatcctc tagcagctgc taggtgctat
     8461 agggggcaag ttttgatcca tttggattac cctaaaaacc tcagctgaac ccgttgcgag
     8521 ggtgattggg gcgccgaaaa tacagggtct aactgaggtt tttaggatta tagttgagga
     8581 tcctaagcgc acaaacttcg caggccgtta gcccttggtt ggagaaggta gacatgcccg
     8641 gtcgattttt acccgcgtgt agaggaaact gggcaatgtc actaaggtaa gccctatcgc
     8701 gctcgctcgt ttcctcctgc gcgaaatcgc aggatccaga tcctctgtgt agaagtgccg
     8761 tatgtttggc tgatgagcac ctaaacccag cttgcttttg gccctatacg gctacccaaa
     8821 tggatcgtct gacttcgcgc aggagaacgg cctccccctt gcaaggccgt aggctggggt
     8881 gattatccca accggtaagt cggattaatc gttggtcgga aaagccgcag gcgccttccg
     8941 acattttggg gcgagcaata tgcttgattt aaaatccaaa ttattgaaaa taatcaacgt
     9001 cggaaggcgc tatgcttttc cgactcgctt cgagtgccgt aggcggtaga gatttagaat
     9061 cgttgagatg tttaatcaat tatatatttt gctgacgggt gcctgcacat gatggataac
     9121 gacgatatat ggataacata cagcgaaaaa tcaaattctt ctattgtggt aggtacccag
     9181 atatttgtaa gccttatgct gttggtggca atcgccaaga taggggcttt gccatcggca
     9241 aagggttggg cgctggtgat atcgctgccg cttatttggt tatttctact aaatttgtca
     9301 ggcacgcaat acacagcggt taattttatt gtccacaaca ttgtggaaga caggacatta
     9361 tttggctcag tctgtacgag actaatttca ttcagtgata taacattttt tgcctatgat
     9421 cctgtcaatg ggcacaaccg cctgaaaatt atactacgat ctggaaaaac acattttttt
     9481 aattttggca acattttgtc gccacgttac caaagactaa tatctgccat cgacgccagc
     9541 aacccaaacc cgtatagatc agctgtccag ctggatgatg catcagttgc atgcctgcaa
     9601 attgcgccca ttcatttcac cccagatcaa atcacacttg atgttaaaaa tcagaagata
     9661 atagtcgggg gcaaacccga aactgtgcca gttgaaatac gtttttccga cataagcaat
     9721 gtgatagtac tcattgatgt agattccgac ccagctgatg aaactaaaac ttttaatcga
     9781 tattgcctat gcgctatttt aaattcattc gagaaaattg aattgacgga ttacgaaacg
     9841 gaccgccaag gtgacgaggt gcgcgataaa atgtgggcgg ctcgcctaca tgttaatgct
     9901 tatctcgaga gggcccacgg caaacgcgta acccggagtg atttttggga ttgcgtaggt
     9961 ccatgagatg gactccccct ctctaacaac ccggcatcga agtgccacac taaacac
//
LOCUS       DAOQGS010000081         9995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3144_length_9995_cov_4.959356, whole genome shotgun sequence.
ACCESSION   DAOQGS010000081 DAOQGS010000000
VERSION     DAOQGS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_9995_cov_4.959356"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            498..2222
                     /locus_tag="PKZ52_04650"
     CDS             498..2222
                     /locus_tag="PKZ52_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG59092.1"
                     /translation="MKAPVSTPRTILLICNNAVTASHITNLLNIPNDNWDELCVVTSL
                     EAALQHIRKQPPLCCLLDTQVKTLSDQTTVVKALRREYASDNLPLLLINPTDGEDDAG
                     QTLAQGAQDYLTLNTMTAAQLSSAIHNAIHTSQLQKQLNNLAHYDSLTGLLNRNLLYN
                     RLSHAVGRCNRYGHSCAVLVIDLNNFKPVNEIYGYDVGDKVLQYVAACIRDNCRSTDS
                     PARMGGDEFVVLLEHVDEQTGQLITEKIVQAIQTPFDVNGISIQLSACAGLATYPVAA
                     SVEELLKHADEALVLAKNGQDQNWVSFSNEHKERWTRQQILERELPKAIANGELALVY
                     QPIVCAKTHQLRRLEALSRWPRSDFAVGAPELINMIDRLQLIEPFHEWLFHTAFKQMH
                     QWKQEAINADICLNIPANYCYRQSIIHTIEQAFSLYHIEPSRVELEITESTLMLYPER
                     SVDLLQRLHDRGIRIAVDDFGTGYSSMAYLTLLPLDTLKIDKQFFIENSKNDRNRKVI
                     EAVTALGHSLGLDIIAEGVETSTELELAKQVGCDLLQGYYFGRPQFAQGSWAEYTQHF
                     NHIAPHTK"
     gene            2475..3800
                     /gene="argA"
                     /locus_tag="PKZ52_04655"
     CDS             2475..3800
                     /gene="argA"
                     /locus_tag="PKZ52_04655"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNG59093.1"
                     /translation="MTNAPDDYVRWFRHSSPYINAHRDKTFVIMLPGNCIEQANLSNI
                     ISDITLLNSLGIRLVVVHGARHQIDEQLAEKNVRSQFHQGLRITSKSDIDQVVAAVGA
                     TRLWLEAGFSNGLPNSPMHGAKIRVRSGNFITAMPQGVIDGVDFQHTGKVRSVDKTGL
                     ISVLATGAIGLISPLGYSATGEIFNLSFADAAVSVAAAIQADKLIAYNDEGRIIDKDG
                     KEYRELTRLQCEKFLIDQPIHSRDNTYFSLRACLKACDSGIARAHVISANEDGSLLKE
                     LFTRDGSGTMVYRDNYETIRLATIEDVVGILNLIEPLEAEGILVKREREILEREIHFF
                     TVMEKDGTIIGCAALYPYSENNAGELACMAVHPDHRRDGRAAKLLKHIERQAVSINLN
                     RLFVLTTQTAHWFIEQGFTACAIDDLPMERRKLYNYQRQSKVFVKTLSA"
     gene            3907..5622
                     /locus_tag="PKZ52_04660"
     CDS             3907..5622
                     /locus_tag="PKZ52_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG59094.1"
                     /translation="MPIGYASLPRMKIEASTPLNDLRQIDIIKIYNKKKCLIIDDFPE
                     IRGNLKRTLRTFGAESVDTAADGEEAVRLCSQNKYDIVICDYNLGVGKDGQQLLEEVR
                     FLRVLLMTSLFVMLTGETSREMVLGALECQPDDYITKPYTEASLKMRLNRAIIRHEAL
                     LPIKQAISDGDYRKAVERCNEFIDKDHQYAPDCIKMKGQLLFLLRQLKEAHQLYESVL
                     AKKPLVWARLGMGKTLLAMGKFDAAENMLEGIIREDERYIEAHDLLADLYTQKNDLIS
                     AQKAVERATHVSPKSVHRHRRLAEVAELNGDDEAAIRSHQNAIRWGVNSCHESPQDYF
                     NYARKVAEHTTGDKSAEAKQLMKQANMFLDRARKRYEGNPEVNAQASMVEAQMFNAQG
                     NEAKAQEHMIKAKNMFNNLRNPSVHTSLEFARTLYSMNEEEQARSILTTVAARHPNDA
                     NLMRIIDGITGEPISDNGKQVAAKLTKTGITAYDSKDYENAINVFNEAIAAYPKHIGL
                     NLNLVQAIVAYTEVSNPSDRLEKMCRRSMRAIGNIPPDHKQFKRYAFIQKQLAKYYPD
                     AVMAA"
     gene            6147..6548
                     /locus_tag="PKZ52_04665"
     CDS             6147..6548
                     /locus_tag="PKZ52_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59095.1"
                     /translation="MKNPQGFTLVELLVGIAIVAILAAVAVPSYSSFVFSGELKTAQS
                     DAKALALQFENRYQRALAYPTVTASTNLSTTFSGWAPASKKFTFSAVGTSTAYTITGA
                     GSGSGYSGCSVVLKEDGTCTVSGCSKGNGSC"
     gene            6548..7036
                     /locus_tag="PKZ52_04670"
     CDS             6548..7036
                     /locus_tag="PKZ52_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59096.1"
                     /translation="MKRSAGFTLVEILIAVALIATLAFAVVPLGNSWTSSAESVNTKG
                     VLSQAISRAKSSAMRNGNKISETNAVTAVCVTSGLVEVREASGSVSSAHCASGGGNTT
                     WSNKIGKTLSIKNNSQTLSCMCFSTKGLLVTSPSNCSSCATTTTFEFSGSGIENETIA
                     IY"
     gene            7014..7658
                     /locus_tag="PKZ52_04675"
     CDS             7014..7658
                     /locus_tag="PKZ52_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59097.1"
                     /translation="MKPLPFTKEPLKNVGGEALARRICQKKRSLHTVNEHFFGKFNDA
                     RASKVIFQRFPKSTRISRDYPNVNFRLDHTANKFMFGSSKIVLTPKFQRGDMLIEALI
                     GLVLMAIVAVGVTTITSKVEKGKREMSVQDLAVSQLQELLVKNGNGSINLCDSTPETI
                     TLPDETITVAASGCTTVSATINGVAFTDLKQPIVLSATSSKNGAKYTVGGVSGQ"
     gene            7684..8310
                     /locus_tag="PKZ52_04680"
     CDS             7684..8310
                     /locus_tag="PKZ52_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59098.1"
                     /translation="MQRNSLFQQGSTLISLLIGMLISLLCIGGSLTLYKNLVRVAAET
                     QSDATYDGQLALSMLTARLELQNAGFGIAGPGTTHIATQTNSSSSSLYWRYKIGSQYY
                     CKGLQDIAVGSNRELRLIDASTSACTETAALTSLTYTNSQTLGEFKNYASPVVSFTVS
                     AQSCWPYGVGKVAPHYQVVISANTAAKQFNSAINASTYTYCLPNIYPT"
     gene            8323..>9995
                     /locus_tag="PKZ52_04685"
     CDS             8323..>9995
                     /locus_tag="PKZ52_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59099.1"
                     /translation="MSASHFIHRSTPKRQRGIATVLIVVLVGLGLTATSMGIMHSVRS
                     TQQKQIASHAITHSQAGAWTGVEVFRRYLASLTTTQLTALALNQSIDFTLPGTTNVVN
                     AVISNRTAPSGSDTTYKLTTRLTYSDIAAKSSAALEVIYEFSPPANNTGVTLSGELNF
                     HRSTTLGGGITVYDAGGTNTGKFNVDGNLDLSSIGLTGVVNLNATGNITLGSSVPALN
                     VKANGVVTLTGSASVYQVEALGGVNITAGVANIVKSNGRVQLSAGNTTEVSTTGNVDV
                     AGWGTHPKISANGNYTHTASATVTLANVGGTSSITTTSNPTLTTLNSIGNVTCSSNSW
                     NNFTSIKTKGTATNCKTGASLQTAQSALTVPTVTALTPYTMTKPRIDVWSLKSSANYI
                     FEYVSGKIRVTTKNVNGLTDGIYYLGVHTYNTTVLYDALCQSVTVAGVCVESSATTKT
                     VCNGQSISNPCFSYDTTLSRFTINSKNVAPGVMWFKGSLLLNNGNYYNTFLATNGIEV
                     SGTTTVTSVNYGGYSNSCSVVYQGAVSFFTGLYPLNFCNTSTSSLIALPL"
BASE COUNT         2762 a         2597 c         2229 g         2407 t
ORIGIN      
        1 gtcaaaaaga gggacctttt tgacgagcct acacggatgt attcacggcg tgtccgcact
       61 aacctgtagt catcactgcg gggctctatt acgggggcac agcactaaaa cacccacgca
      121 tgagctccca gtcagtgcag gaattacccc cagccacata aaaagattct gaatagttac
      181 aatacttatg gagcgctgca atttagtttg cattcgttaa acgcggatgc tgctaggcgc
      241 tggcaaaaaa cacctcaaac caagcatttt tataactatt tatacaccta acgtcttgcc
      301 ttagacgcca ataaattccc acccattaca cagcttgagc ccaacttgca gcgcggctta
      361 gctaggctat gctcttgatt acatcgccca atttcgctct cgtttggctg gagtcaacaa
      421 cacgtggtat caagtactct atctgaaata gtaattggct gcgatcatgg gccacaggct
      481 tgctcaagta ttcagctatg aaagcgcctg tcagcacccc gcgcactatt ttgctgatct
      541 gtaataatgc ggttacagcc agtcatataa ccaacttgct gaatattcct aatgataatt
      601 gggatgagtt atgcgtggtg acaagcttag aagcggcact gcagcatatc cgcaaacagc
      661 cgccactgtg ctgcctgctc gatacccagg taaaaaccct gagcgatcaa acaacagtgg
      721 tgaaagccct gcgacgggaa tatgccagcg ataatttgcc attgctgctg attaacccca
      781 ccgacggcga ggacgatgcc ggccaaaccc tcgcccaggg cgcacaagat tatttgactt
      841 taaataccat gaccgccgcg caattatcca gcgccattca caatgccatt cacaccagcc
      901 aattacaaaa acagctgaat aatcttgccc actacgacag cctgaccggt ttattaaatc
      961 gcaatttact ctataaccgt ttatcccacg ccgtggggcg ctgcaatcgc tacggtcaca
     1021 gctgtgcggt gttagttatt gacttaaata attttaaacc cgtcaatgaa atttacggct
     1081 acgacgtagg cgataaagta ttgcaatatg tagccgcctg catacgcgat aattgccgca
     1141 gtacagacag cccagcccgt atgggtggtg atgaatttgt ggttttatta gagcatgtag
     1201 atgaacaaac cgggcagctc atcaccgaaa aaattgtgca ggctattcaa actccgtttg
     1261 acgtaaacgg catcagtatt caattaagtg cctgtgcagg gcttgctacc taccctgtcg
     1321 cggcaagtgt tgaggaatta cttaaacacg ccgatgaggc gctagtgctc gccaaaaatg
     1381 gccaagatca gaattgggtg agtttttcca acgagcataa agaacgctgg acacgccaac
     1441 aaatactgga gcgggaatta cccaaagcca tagccaatgg cgaattggcg ctggtgtacc
     1501 agcccattgt atgcgccaaa acccatcaat tgcgccgctt agaagcctta agccgctggc
     1561 cccgcagcga ttttgccgta ggtgcaccgg aactgattaa tatgatcgac cggctgcaat
     1621 taattgagcc gtttcacgaa tggttattcc acacggcatt taaacaaatg caccagtgga
     1681 agcaagaggc gattaacgct gatatatgcc tgaatattcc cgccaattat tgttatcggc
     1741 aatcgattat tcacaccatt gaacaagcct tttcccttta tcatatcgag ccctcccgcg
     1801 tggaattgga aattaccgag tccaccttaa tgctctaccc cgagcgcagc gtggatcttt
     1861 tgcagcgcct acacgataga ggcattcgca tagccgtgga cgattttggc actggctatt
     1921 cctcgatggc ttatcttacg ctgttaccct tggatacatt aaaaattgac aaacaatttt
     1981 ttattgaaaa ttccaaaaac gatcgcaacc gcaaagtgat tgaagcggtg actgcgctcg
     2041 gccacagcct aggcttagat attattgccg agggagtgga aaccagtacc gaattggaat
     2101 tggccaaaca ggtaggctgt gatttactgc agggctatta tttcggtcgc ccgcaatttg
     2161 cccagggcag ttgggctgaa tatacccagc actttaatca catagcgccg cataccaaat
     2221 agcgccatcg atcaaataac acagcgatcc attagcgcat tcgatcgatt agcaccgcca
     2281 aatcagagct gcgcccacaa tattgcgccg cgcggtaacg cgctgccgca ggcaggtgcc
     2341 accctccctc caacgcgctc ccagcaagcg cccaatccgc accagatgcc aagaaattgc
     2401 acagaaaccc gccccatccg cttactgagc taggtataat cagccgcttt gccctgtacg
     2461 tttgagacac cctaatgacc aatgcacccg atgactacgt tcgctggttt cgccattcct
     2521 ccccctacat taatgcgcac cgggataaaa cctttgtgat tatgctgccg ggcaattgta
     2581 ttgagcaggc gaatctgtcg aatattattt ccgatattac cttgctcaat agtttgggca
     2641 ttcggcttgt ggtggtgcat ggtgcgcggc atcaaatcga cgagcaatta gcggaaaaaa
     2701 acgtgcgctc gcagtttcac caggggctgc gcattacctc taaaagcgat atagatcagg
     2761 tggtggccgc cgtgggcgct acacgcctgt ggttggaagc gggtttttcc aacggcttgc
     2821 ccaactcccc catgcacggc gccaaaatac gcgtgcgcag cggcaatttt attaccgcta
     2881 tgccccaggg ggtcattgac ggtgtggatt ttcaacacac aggcaaagtg cgctcggtgg
     2941 ataaaaccgg cctcatcagc gtactggcta ctggcgccat cggccttatt tcaccgctgg
     3001 gctattccgc cactggcgaa atttttaatt taagttttgc cgatgccgcc gtcagcgtcg
     3061 ccgccgccat tcaagcggat aaattaatcg cctataacga tgagggccgc attatcgaca
     3121 aagacggcaa ggaataccgc gaattaactc gcctgcaatg cgagaaattt ctcatcgatc
     3181 agcccataca cagccgggat aacacctatt tttccctgcg cgcctgcctg aaagcctgcg
     3241 atagcggcat cgcccgcgcc catgtgattt ccgccaatga agacggctcg ttattaaagg
     3301 aattatttac ccgcgatggc tccggcacca tggtgtaccg cgataattac gagaccattc
     3361 gcttggcaac gattgaagat gtagtcggca tcttaaattt aattgagcca ttagaagccg
     3421 aaggtatttt ggttaaacgg gagcgggaga tactcgagcg ggagattcat ttttttactg
     3481 ttatggaaaa agacggcacc attataggct gcgccgcgct gtacccttac agcgaaaaca
     3541 atgccggtga actggcctgc atggccgtgc atccggatca tcgccgggat ggccgcgccg
     3601 caaaactgct aaaacacatt gaacgccaag ccgtgagtat taatttaaat cgcctatttg
     3661 tgctcaccac tcaaaccgcc cattggttta ttgagcaggg ttttaccgcc tgtgccattg
     3721 acgacttacc catggagcgg cgtaaactct ataactacca acggcaatcc aaagtgtttg
     3781 taaaaaccct aagcgcataa tacttcaccc ctccggcgca caggcgccgg attgctctct
     3841 cgcctacgcc tccctgctcc gccgccaggt attgttaata atgcctgcta cactgatggt
     3901 taaaccgtgc ctattggtta tgcgagtcta cccaggatga aaattgaagc cagcacccca
     3961 ctgaatgatt tacgtcagat cgacattatt aaaatttaca acaagaaaaa gtgtctaatt
     4021 attgacgact tcccagaaat acggggcaac cttaaaagga cgttgcgcac gtttggcgca
     4081 gaatccgtgg ataccgccgc cgatggcgaa gaagccgtgc gcctgtgcag ccaaaacaaa
     4141 tacgatattg taatttgtga ttacaacctg ggtgtaggta aagacggcca acaattattg
     4201 gaagaagtgc ggtttttacg agtcctctta atgactagct tatttgtaat gctcacgggt
     4261 gaaacctcgc gggaaatggt attaggcgcc ttggagtgcc aaccggatga ctacatcacc
     4321 aaaccctata ccgaagccag tctaaaaatg cgcttaaatc gcgccatcat tcgccatgag
     4381 gcactgcttc ccatcaaaca agcgatctcc gatggggact atcgcaaggc ggtcgaacgc
     4441 tgtaacgaat ttatcgataa agaccatcaa tacgcgcctg attgcatcaa gatgaaaggc
     4501 cagttgttat ttttactgcg ccaactaaaa gaagctcatc agctgtatga atccgttttg
     4561 gcgaaaaaac ccttggtgtg ggcgcggctg ggtatgggta aaacattatt agccatgggt
     4621 aaatttgatg ccgcggaaaa tatgctcgaa ggcattatcc gcgaagacga gcgttatatc
     4681 gaagcccacg atctattagc cgacttatat actcagaaaa acgatttaat cagtgcgcaa
     4741 aaggcagttg aacgagccac ccatgtatca cccaaatccg ttcatcgcca ccgccgctta
     4801 gcggaagtag ccgaattaaa tggtgatgac gaggccgcca ttcgctccca ccaaaacgcc
     4861 atccgctggg gcgttaattc ctgccatgaa tccccgcaag actattttaa ttacgcccgc
     4921 aaagtggccg agcacaccac cggcgataaa tccgccgaag caaaacaatt gatgaaacag
     4981 gccaatatgt ttttagatcg cgcccgtaag cgctatgagg gcaacccgga agttaacgcc
     5041 caggccagca tggtggaagc acaaatgttt aacgcccagg gcaatgaagc aaaagcccaa
     5101 gagcatatga tcaaagctaa aaatatgttt aataacctgc gcaatccttc ggtgcacacc
     5161 agtttagagt tcgcccgcac cctctattca atgaatgaag aggaacaagc ccgcagtatt
     5221 ttaacgactg tggccgcccg ccaccccaac gatgcgaatt taatgcgcat tatcgatggc
     5281 atcaccggag agcctattag cgataacggc aaacaggtcg cagccaaact caccaaaacc
     5341 ggcattaccg cctacgattc caaagattat gaaaatgcca ttaatgtatt taacgaagcc
     5401 attgccgctt atcctaaaca tatcggctta aatctaaacc tggtacaggc tatagttgcc
     5461 tacacggaag tgagcaaccc cagcgatcgc ctagaaaaaa tgtgccgccg cagtatgcgg
     5521 gccatcggca atattccacc agaccacaaa cagtttaagc gctatgcatt tatccaaaaa
     5581 caattagcca aatattaccc cgacgcggtc atggccgcct aaattgccgg ttattcgcaa
     5641 actctgctct tagctgggca cggctgttgg ccgcgcccag tttactcaac acaaagcatc
     5701 ctctcgccag cgcctaccat tcatcggtgc tcatgcattt taaataacgc ctataaatac
     5761 ttacccacag cgcgccaaaa aaactctgtt tttaccttgc cagcattgtc tggttagcgc
     5821 accacttttc gctgccaact cattgacatc gacacgaaaa aatctaaacg gcatttttta
     5881 acgctaaata attggcgccc aaccccaggt ctgggcaatt ttttagttat taaatctaat
     5941 ccaaacgcct taaaaacatc agtcttatac cctccttaca aatagaaact gcaatcgaga
     6001 ttatttattc atatccacta tatgctcttt gtatttaaag gctatgccat caatacttta
     6061 gctaaagatc acaatgatcg gagcgccaaa cgagtccgag gcatgtgcct cactctctgc
     6121 tagccgataa aaaagacgag actcacatga aaaacccaca aggatttacc ttagtcgaat
     6181 tattagttgg tatcgccata gtggctattt tggccgctgt agccgtgccg agctatagca
     6241 gttttgtatt ttccggtgaa ttaaaaaccg ctcaatccga tgcaaaggcc ctcgccctgc
     6301 agtttgaaaa ccgctaccaa cgggcgctgg cctaccccac ggtcaccgcc agcacgaatt
     6361 tatccaccac cttttccggc tgggcacctg cttccaaaaa atttaccttt tccgccgtgg
     6421 gcactagtac ggcctacacc attacaggtg cgggctctgg ctcaggttat agcggttgta
     6481 gcgtcgtgct gaaagaagat ggcacttgca cggtttccgg ctgcagcaaa ggtaatggga
     6541 gctgctaatg aaacgctcgg caggttttac actggttgaa atattaattg ccgttgcatt
     6601 gattgccact ttggcttttg cagtcgtgcc actcggcaat tcatggacca gcagcgccga
     6661 atcggtcaat accaaaggag ttttatctca agcgatcagc cgtgcgaaat cgtccgccat
     6721 gcgcaatggc aataaaattt ctgaaaccaa cgcggtgact gcggtatgtg tgaccagcgg
     6781 cctagtggaa gtgcgtgaag cgagcggctc ggtgagcagc gcacactgcg cctcaggtgg
     6841 tggcaatacc acctggtcta acaaaattgg caaaacctta agtattaaga acaacagcca
     6901 aacacttagc tgtatgtgtt tctccaccaa gggcttatta gttacctcac cgagcaactg
     6961 cagcagctgc gccaccacca caacgtttga atttagcgga tcaggcattg aaaatgaaac
     7021 cattgccatt tactaaggaa cctctgaaaa atgtaggcgg cgaagcgctg gctaggcgaa
     7081 tttgccaaaa aaagcgcagt ttacacacag taaatgagca tttttttggc aaattcaacg
     7141 acgccagagc gagtaaggta atttttcaga ggtttcctaa atccactcgc attagccgag
     7201 attatcccaa tgtaaatttc cggcttgacc atacagccaa taaattcatg ttcggcagca
     7261 gcaaaattgt attgacgccc aaattccaac gcggcgatat gctgatagag gctttaattg
     7321 gcttggtgtt aatggccata gtagccgtcg gtgtaaccac tattacctct aaggtggaga
     7381 aaggcaaacg ggaaatgtcg gtacaggatt tggcggttag ccaactccag gagttattgg
     7441 tcaaaaacgg taatggcagt attaatctct gcgatagcac acctgaaaca atcaccctac
     7501 cggatgaaac catcacggta gccgcctcag ggtgcacaac cgtttccgcc actattaatg
     7561 gcgttgcttt tactgattta aaacagccca tagtgctctc agccaccagc agtaaaaacg
     7621 gcgcaaaata cactgtaggt ggtgtttctg ggcaatgaaa tcgtgtcgcc aactaggtta
     7681 accatgcaac gaaatagtct atttcaacag ggctcaactc taattagctt gctaatcggc
     7741 atgttaattt cgcttttgtg tattggcggc agcctcacac tctataaaaa tcttgtccgt
     7801 gtggccgccg agacccaaag tgatgccacc tatgacgggc aattagcact ctcaatgctc
     7861 accgcgcggc tggaattaca aaatgcgggc tttggcattg ctggcccagg tacaacccat
     7921 atcgccacac aaaccaatag ctcgagcagt tctctgtatt ggcgctataa aataggcagt
     7981 caatattatt gcaaaggctt acaagatata gccgtaggca gcaatcgaga attgcgctta
     8041 atcgatgcca gcacctctgc ctgcactgaa acggcagccc tgacctctct tacttatacc
     8101 aacagccaaa ccctaggtga atttaaaaat tacgccagcc cagtggtgtc atttactgta
     8161 tccgcgcaat cgtgttggcc atatggcgta ggtaaagttg cacctcatta ccaagtggta
     8221 atttcagcca acactgctgc aaaacaattt aattctgcca ttaatgcatc cacttatacc
     8281 tattgtttac ccaatattta tccgacctaa ttagggcttg ccatgtctgc gtcacatttt
     8341 attcaccgct cgacacctaa gcgccaacga gggattgcca ccgttttaat tgttgtatta
     8401 gtggggctgg gattaactgc cacctctatg ggtattatgc attcggtgcg tagcacccaa
     8461 caaaaacaaa ttgcctccca tgctattact cattcgcagg caggggcctg gacaggggta
     8521 gaagtatttc gccgctattt agcgtcgctc acaacaacgc aattaaccgc cttagcctta
     8581 aatcaatcca ttgattttac gctgccaggc accaccaatg tagtaaatgc cgtcatttca
     8641 aaccgcaccg ctccctcagg ctccgacact acatataagc tgacaacgcg cttaacctac
     8701 agcgatatag ccgctaaaag tagcgccgca ctggaagtta tttatgaatt ttcaccgcct
     8761 gccaataata ctggcgtgac gctcagtggc gaattaaatt tccaccgcag taccaccctt
     8821 ggcggcggca tcaccgtcta cgacgcgggg ggcactaaca caggtaaatt taatgtcgat
     8881 ggtaatttag atttgtccag cattggttta accggtgtgg tgaatttaaa tgccaccggc
     8941 aatattaccc ttggcagctc cgtaccagct ctaaatgtaa aagccaacgg ggttgtaacc
     9001 ctgaccggtt cagcctctgt ttatcaggta gaagcactag gcggcgttaa tatcacggcg
     9061 ggggttgcca atattgtcaa gagcaacggc cgggtgcaat taagcgcagg taacactaca
     9121 gaggtttcaa ccaccggtaa tgtggatgta gctggctggg gcacgcaccc gaaaattagc
     9181 gcgaatggca attacaccca caccgcaagt gccacagtga ccctggcgaa tgtaggcggc
     9241 accagttcga ttaccaccac ctcaaatccc acgctcacaa cactcaatag tattggcaat
     9301 gtcacttgtt caagtaatag ctggaataat ttcaccagca ttaaaaccaa gggtacagca
     9361 accaactgta aaacgggtgc cagcttacaa actgcacaat cggcgctgac ggttcccaca
     9421 gtcacagctt taactcctta cactatgacg aaaccgcgca ttgatgtgtg gtcgcttaaa
     9481 agctcggcaa attatatttt tgaatacgta tcaggcaaaa taagagtcac caccaaaaat
     9541 gttaatggcc tgactgacgg catttattat ttgggtgtgc atacctataa caccacagtg
     9601 ctgtacgacg cactttgcca atctgtaacc gtagcaggag tttgcgtcga gagtagcgcc
     9661 acaaccaaaa ccgtttgcaa tggacagtca attagcaatc cgtgttttag ttacgacaca
     9721 acactcagcc gctttaccat caattctaaa aacgtagcgc cgggtgtgat gtggtttaag
     9781 ggcagtttgc tgttaaataa cggcaattat tacaatacct ttctcgccac caacgggatt
     9841 gaagtttccg gcaccaccac cgtgacatca gtaaattatg gtggttatag caatagctgc
     9901 agcgtagttt accagggtgc cgtcagcttt tttaccggtt tatatcctct gaatttttgc
     9961 aataccagca catcgagttt aattgcactg ccact
//
LOCUS       DAOQGS010000082         9980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3150_length_9980_cov_4.705290, whole genome shotgun sequence.
ACCESSION   DAOQGS010000082 DAOQGS010000000
VERSION     DAOQGS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_9980_cov_4.705290"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKZ52_04690"
     CDS             <1..232
                     /locus_tag="PKZ52_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59100.1"
                     /translation="AVGAPATNQISGRIVCLSQVPGKSAVALDTQLDDGNGDTGKMRA
                     FLGTAGTNTAPSNTALGAAYNETSEYTICLQM"
     gene            complement(447..1901)
                     /locus_tag="PKZ52_04695"
     CDS             complement(447..1901)
                     /locus_tag="PKZ52_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNG59101.1"
                     /translation="MKIKALVTALLAAAAAPSFALVPGAEGNSNPVLHVYGTGATAID
                     KQLKQLVKTICKAGTLDIFTDDVTKPEGNKYAAYYCVVSPTEVPGISATYNLFIHKNS
                     NGGSFNGVGPVADSLIEDQMVIAGNCGAAAAGSDAGTPVWACNKANVDNTQLPDFGIA
                     DVEPGLWAKLGQSFTASNLVSTAMNGNTFGIPVTKALRDALQTAQGLPSGSELAADQP
                     NLSRADVASILAGNITSWDQFKVNGVALPANSLVAPVNKRVQVCRRVDTSGTQAQANV
                     FFHNYPCDSSASKTANDNTSFSSTADGKTADDNLNPLPATSAVVHQFDGSGGVSDCLS
                     VLQDANAWGVGILSLERSSSKYRFVKIDGALPTLKNVANNTYGEWYTSTIQWRAASAP
                     NAPTGDKLKVLELIRNDASSPAILAQINASFTQSWTTGPGEVGLVALPYKSSVTNKVF
                     SVANPVATASREGKTGTGIPNSCKTPLIVNNAEI"
     gene            complement(1971..2768)
                     /locus_tag="PKZ52_04700"
     CDS             complement(1971..2768)
                     /locus_tag="PKZ52_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNG59102.1"
                     /translation="MKIKALLAAALVATSSASFASVDDGIDPATLTGGELILAAWDDV
                     AKKSILFDTGVTFKQILDAPAPFSVNLNTIDATYKSFFNNNFTNVYWNAYVGSWYGDA
                     VGYQDAKYFGAAYTSKDPDFEIANPLGYNDVVALITGPGEAVSRFNENFVSPKDTTAT
                     SVNRSYKANDATAPNYVGNLTQLWGDTQGQSNGRGTSAKAGEAIAFVYNGTADFDTGF
                     VKKLGTLKFDPTTDSLRINTPLPAAAWLLMSGIAGFGAIARRRKQQA"
     gene            complement(3442..5835)
                     /locus_tag="PKZ52_04705"
     CDS             complement(3442..5835)
                     /locus_tag="PKZ52_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG59103.1"
                     /translation="MRLRASLSLSLCLSAIILVAGCDNKTDRKTRYLNQGKEQLEAKN
                     YEKARISFKNVLQIDPNDQEAMYQFGKLSEAKQEFQQAFGYYNKVVELNPNHIGANLA
                     LTKFYLAANVLDKAENTLSTAETAEPNNLDAKVLRAALFVRHNNNERGRDIVNEVLNT
                     NPTHRDALAMLAGMHSDAGRDDEAQATLDRALETYPDNATFLTLQSRLYLKRGQADLA
                     WDTYEKIAQLEPDNFTKTTEIVKIMLQNQQRERALGLLDKFISNNPENTDAKVAKVEL
                     LQLFGRKDDALALLDTYIKQNPKEFIFGLTKASYALQAGQRSQGIEQLTLISRDAAGT
                     PSELRALNILARVYLEDKQLEQAKAAVDTVLSKSAKDLDALEVRGILALNRKDFTQAI
                     GDFRAVIADKPTQTKIYQALANAHTANGEAELALTVLRDGVKANPTNITLHIELANQL
                     KRANDIKGAESHFEEAVKLDSHNAKALDGLVNIYLEQKKGAAIRTYSEPLQADTELGL
                     LTRYYTALSYSVDDKPQKALELFDALLKEKADFIEVVSARAKTMLQMGQNAAARNWLR
                     QQLPNLPKDQAVIYNLMGELALNDKEYSQAIENFQQAIKLQDTWAIPYSHLANAQRKA
                     NNTAAAIATLQAGLGKQPNHLLLLNDLASLYEADKQPDKAIQLYDTFYKNNGQPEIIG
                     NNLAMLLATYRTDSQSNQLALSIADQLRSSKNPLYLDTAGWVYFKQGRIIDAKTILLD
                     AQSKFDTPVIHYHLGAVYLAEKDKTSAKYHLEKAQTDKSNYPGRSEVDSLLNTLAQE"
     gene            complement(6005..7636)
                     /locus_tag="PKZ52_04710"
     CDS             complement(6005..7636)
                     /locus_tag="PKZ52_04710"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNG59104.1"
                     /translation="MILFICFIVAMLLSLSFIPPLIKFSPSLKLIDAPNERKVHQKLI
                     PRAGGIAIVCGALIPMLIWLPAQPSNSSLFLAIIVLLAFGIWDDRADLDYRLKLLGQC
                     LSAAIVILGADIRIVTLPFLPDMQLPLIMSQILSLVVLVGVINAINLVDGLDGLAGGS
                     TLVGFSLIAFLSMQAGDLGLTLISLSVVGALMGFLRFNTHPAIIFMGDSGSQFLGFIA
                     GITSLRLTQQADVGISPLFPLLLFAIPIFDTCAVIARRLAQGRSPFSPDRNHIHHRLL
                     AAGLDHYETVVLIYVLQGLLALLGFYFRYHTDIELLLILVSVLIVLFGGLNWCDRHPN
                     RLGRLLAPHMAVTPTRHQLKDGLLLLGRYCLAFLLCALLVQSALFTAVRNSDLFILIV
                     GLTLLFLGAGFINRSQPTLAWPERMGIYVLCSTAIFFYYHIPVDFTAQKSLIFLAFLG
                     IFLFTIIGLQFSRRHSFELSPTDYLVIFITLTLAFYPVADPQTRNLTISISFLIGLFY
                     TMEYVLNSHIFSTVQLRAGLSISCILTLFALQRLI"
     gene            complement(7646..9232)
                     /gene="xrtD"
                     /locus_tag="PKZ52_04715"
     CDS             complement(7646..9232)
                     /gene="xrtD"
                     /locus_tag="PKZ52_04715"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HNG59105.1"
                     /translation="MHPNHRTPTVYWHSPLTLWLSFATAFTALAWVFATGLENLWFNW
                     HHKEEYSHGILLPFISLFLIWQKKHDIAAEPFVGSWAGLGLFVLGTLGCFAGAMSTLF
                     PVMQYSLLISLGGLTLAITGWRAFRFFIVPLLILLLSVPLPNFIYKNLSFELQLLSSS
                     IGVHFIRLFNIPVYLEGNVIDLGTMKLQVVEACSGLRYLFPLMTLGFIAAHFFKVPLW
                     KKILVFLSTIPITVLMNSLRIGIIGVTVEWWGQAMAEGFLHDFEGWVVFMGCTAILFA
                     EMALIARLSTPRAHLIDLLMIELPTDRVKQAVAEQNRTLPLSYVIACALALVVSAVAY
                     FTPEAGLKTISRAPFSSFELELGHWSGVRAPIEQRYIDELKFDDYLSVDYKNNHQDAI
                     NLYVAFYNSQSAGQSAHSPRSCIPAGGWEIQNAATINLPNVKSPGVDLEINRLQVQKG
                     DTRLMVYYFFNQRHRHLTNEFLVKWYLMWDAITLHRKDGALVRISFVTDRNTTLEQAD
                     QRAIDFLTLAYPKLNRHIPD"
     gene            complement(9239..>9980)
                     /locus_tag="PKZ52_04720"
     CDS             complement(9239..>9980)
                     /locus_tag="PKZ52_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNG59106.1"
                     /translation="HILARGGKADKLSVIYNGVDLSLFDCAEVQSKADAAQYFGVHYE
                     PSMFFAAYVGTHGMAHHLETILEAAEILKDHPSIRFLMAGDGSEKAKLVKIKQEKSLH
                     NVIMLDQLPKADMPRLWQLVDTSLVLLKKSDLFKTVIPSKIFESMAMKRPIVLGVEGE
                     VADIINESESGICITPEDASQLADAVEHLHDHRAYAELLGENGLAYVARTFERSQLAL
                     QFMHILEGIDRRRREMFSPSPLASRQLP"
BASE COUNT         2694 a         2228 c         2534 g         2524 t
ORIGIN      
        1 cgccgtcggc gcgccagcga ctaatcaaat ttccggccgc atcgtctgcc tcagccaagt
       61 gccaggcaaa tccgccgtgg cattagatac tcagctggat gatggcaatg gcgacaccgg
      121 taaaatgcgc gcatttttag gtacggcagg taccaatact gcacccagta ataccgcgct
      181 tggcgcagcc tataacgaaa cctctgaata tactatttgc ctacaaatgt aattaagtcg
      241 ctgcaggcag aaagcccgta gcggcccgcc tggggaagct gcagaggata tatttagcga
      301 ttgcagacta tttacctatt caggttttat cagagcattt tgttaacttt aaaaaataaa
      361 aaagccagca ttgtgctggc ttttttattt ccttaaggat ttgcactcgc catccctggc
      421 ctttaagagt cgattgatta aaacgcttag atttcagcgt tgtttacaat cagaggagtc
      481 ttacatgagt ttggaatgcc agtaccagtt ttgccttcgc gagaagcggt ggctactggg
      541 ttggcaacgc taaatacttt gttggttaca gaagacttat aaggcagagc cactaagcct
      601 acttcacctg ggccagtggt ccaagattga gtgaacgaag cattgatttg cgccaaaata
      661 gcagggcttg aagcatcgtt acggatcagc tcaagcactt ttaatttgtc gcccgtagga
      721 gcgttggggg cagaagcggc gcgccattgg atagtagagg tgtaccactc gccgtaggtg
      781 ttgttagcca cgtttttcaa agtaggcagg gcgccgtcaa ttttaacgaa gcggtatttg
      841 ctgctgctgc gctcaagcga caggatacct acaccccagg cattggcatc ttgtaacacg
      901 cttaaacaat cagaaacgcc acctgaacca tcaaactggt gtaccacggc agaagtcgct
      961 ggcagtgggt tcaggttgtc atcagcggtt ttaccgtcgg cggttgagct gaaagaagta
     1021 ttgtcgttgg cagttttgct ggccgatgaa tcgcaaggat agttgtgaaa gaacacgtta
     1081 gcctgagctt gagtacctga ggtatcaacg cggcggcata cttgtacacg cttgttcact
     1141 ggagcaacta aagagttggc tggcagagct acgccgttca ctttaaattg atcccagctg
     1201 gtgatgttac cggccaggat actggctacg tcagcgcgag acaggttggg ttggtcagcc
     1261 gctaactcag aaccgcttgg caggccttga gcagtttgca gcgcatcgcg cagagctttg
     1321 gtaacaggaa taccaaaggt attaccgttc atggcggtgc ttaccaagtt agaagccgta
     1381 aagctttgac ctaattttgc ccacaagccg ggctcaacgt cggcaatacc gaagtcgggc
     1441 agctgagtgt tatcaacgtt ggctttgtta caggcccata ctggagtgcc agcgtcgcta
     1501 cctgcggcag cggcgccaca gttaccggcg atcaccattt gatcttcgat taaagaatca
     1561 gctacagggc caacaccgtt gaaagagcca ccgttggagt ttttgtggat aaacaggtta
     1621 taggtagcgc tgatgccggg cacttcggta gggcttacta cacagtagta ggcagcgtat
     1681 ttgttgcctt caggcttagt tacgtcgtca gtgaagatat ccaaagtgcc agctttacaa
     1741 atggttttaa ccaattgttt taattgtttg tcgatggcgg ttgcacctgt gccatagacg
     1801 tgtaaaacgg ggttgctatt gccttctgcg ccgggcacta aagcaaaaga tggggcggca
     1861 gcggcggcca aaagggcggt aacaagagct ttaattttca tgggaatctc caaaattttg
     1921 ggcaaacaga aaccggcggc ggaccgccgg tgggaaacta aacgcttaaa ttaagcttgt
     1981 tgcttacgac gacgagcaat agcgccgaaa cctgcgatgc cagacattaa caaccaagca
     2041 gcggctggta atggagtgtt gatgcgcagg gaatcggtag taggatcaaa tttcaaagtg
     2101 cccaattttt ttacaaaacc tgtatcaaaa tctgcagtgc cgttgtagac gaaagcgatg
     2161 gcttcaccgg ctttagcgct ggtgccacgg ccgttagatt gaccctgtgt gtcgccccac
     2221 aattgagtca ggttgcccac gtagttaggt gcagttgcat cattggcttt gtagctgcgg
     2281 ttaacggaag tggcagtagt atctttgggc gatacaaaat tctcgttaaa acggctaaca
     2341 gcttcgcctg gaccagtgat taaagccacc acatcgttgt agccgagtgg gttagcaatt
     2401 tcaaagtctg gatctttaga cgtatacgca gcaccgaagt attttgcatc ttggtaacca
     2461 acagcgtcgc cgtaccagct acctacatag gcgttccaat aaacgtttgt aaaattgtta
     2521 ttgaaaaagc tcttataggt tgcatcgata gtgttaaggt taactgagaa aggtgcagga
     2581 gcgtcaagga tttgcttaaa cgtcacacct gtgtcgaaca ggatagattt ctttgcaacg
     2641 tcatcccatg cagcaagaat cagctcgcca ccagttaagg ttgccggatc aataccatca
     2701 tccacgctag cgaatgaagc agaagaagtt gccactaaag cggcagctaa taaggcttta
     2761 attttcatgt ttgactccat aaagggtttt ggaatttcag gttgttaata caacttagag
     2821 atttcgtccc gaggtcctta taaaaatcat gagcctaaaa agtgactcgg tgcaatagtc
     2881 cgttgcagct gggcggtgcg ttataacgct tgcgcatact cacaaaagtt ggttaaggtt
     2941 caatggctaa tttatgaatt ttttcgcatg aaattgttac caatatgaca gaggccgagc
     3001 agagctaaac cgcgtagggc aagacgcagc tgccacgttt tacacttgga gacagcctta
     3061 aatacgggtg gaggaacgcg gtggagacaa gaggtttgat ggcgctagga tcaaggaggc
     3121 taatatcaag ctgccagaag cttaataatt agaaaataaa gcgttcgctg atggggacga
     3181 attggtagcg gtcgcacgcg cgtataggta cataccagcg gcttaatttg caggcgtacc
     3241 cgctatggtt gcaaaataat attgaatcca aatattaacc ttgcggcaga atggttcgcc
     3301 atgatccatt gtgccctcat tccgccgaca cttgcgaaca cgcttgaatt ttcgtgtttt
     3361 agcggagcgt gtggcacagg tgcggcatcc ctgccgccat taaaatctgc gcctgccgta
     3421 tcacagcgct tgtgcgcgtg attattcctg ggcaagtgta ttgagtagcg aatctacttc
     3481 actgcggcca gggtaattgc ttttatccgt ttgggctttt tccaaatgat atttggccga
     3541 tgttttatct ttttcagcca gataaactgc gcctaggtgg tagtgaatta ctggagtgtc
     3601 gaatttgctt tgtgcatcta gcaaaatggt ttttgcatca ataatacgcc cctgtttaaa
     3661 ataaacccag cctgcggtat ccaaatacaa agggttttta ctgctgcgca gttggtcggc
     3721 aatgcttagc gccagctgat tgctttggct gtcggtgcgg taggtggcta ataacatggc
     3781 taaattattg ccgataattt ccggctggcc attgtttttg tagaaggtgt cgtacagttg
     3841 aatggctttg tccggttgtt tgtccgcctc atagagagag gctaaatcgt tcagcaataa
     3901 taagtgattg ggttgtttgc caaggcctgc ttgcaaagta gctatggcgg ctgccgtgtt
     3961 gttggctttg cgctgggcgt tggccaagtg agaataggga atggcccagg tgtcttgtaa
     4021 tttaatggcc tgctggaaat tctctatggc ctgtgaatac tccttatcgt taagcgccaa
     4081 ttcacccatt aaattgtaga taacggcttg atccttcggc agattgggca attgctggcg
     4141 cagccaattg cgcgccgccg cattctgccc catttgtagc atggtttttg cccgtgcaga
     4201 cactacctcg ataaaatcgg ccttttcttt tagcagggca tcaaataatt ccagtgcttt
     4261 ttggggtttg tcgtcaacgg agtaggacag ggcggtgtaa taacgggtca gcaggccaag
     4321 ttcagtatcc gcttgcaatg gctctgaata agtgcgaatg gccgcgccct ttttctgctc
     4381 caaataaata ttaaccaaac cgtcgagggc tttagcattg tgtgaatcga gttttaccgc
     4441 ttcttcaaaa tggctttcgg cgccttttat atcgttagcg cgtttgagtt gattggccaa
     4501 ttcaatatgc agcgttatat tagttggatt ggctttaacg ccgtcgcgca gaacggttag
     4561 ggctaattcg gcttcaccat tggccgtatg agcattggca agagcctggt aaattttagt
     4621 ttgcgtcggt ttatcggcaa tgaccgctct aaaatcgcca atggcttggg tgaaatcctt
     4681 gcgattaagc gccaaaatac cgcgcacttc taatgcgtct aaatctttcg cacttttgct
     4741 gagcaccgta tccactgccg cttttgcctg ttccagctgt ttatcctcca ggtaaacgcg
     4801 ggccaatata ttcagcgcgc gcaattcgct gggcgtgcca gcggcatcgc ggctgatgag
     4861 tgttaattgt tcaattccct gggagcgctg gcctgcttgt aaggcgtagg acgctttggt
     4921 caagccaaaa atgaattctt ttgggttttg tttgatatag gtatccagca gtgccagggc
     4981 gtcatctttg cgcccgaata attgcagcaa ttccaccttg gcgaccttgg cgtcggtatt
     5041 ttcagggttg ttgctaataa atttatccag taggcccaat gcgcgctcgc gctgctggtt
     5101 ttgcagcatt attttgacta tttcggtggt tttggtgaag ttgtcgggtt ctagctgagc
     5161 aattttttca taggtatccc aggctaaatc cgcttggccg cgttttagat ataaacggct
     5221 ctgcagagtt aaaaatgttg cattatcggg gtaggtttct aacgctctat ccaatgtagc
     5281 ttgcgcttca tcatctcgcc cggcatcgga gtgcataccg gcaagcatgg cgagtgcatc
     5341 ccggtgagtc gggtttgtat tgagcacttc attaacaata tcccggccgc gctcgttatt
     5401 attgtggcgg acaaataaag ccgcccgtaa aacttttgcg tccaggttat taggctcggc
     5461 cgtttctgca gtgctcaagg tgttttcggc tttgtccaat acgttggcgg ctaaataaaa
     5521 tttggtcagt gccaaattgg cgccaatatg attgggattt aattccacca ctttattgta
     5581 gtagccaaat gcctgctgaa actcctgttt ggcttcactg agtttaccaa actgatacat
     5641 ggcttcttga tcattcggat caatttgcaa gacattttta aaactgatgc gggccttttc
     5701 ataattttta gcctccagct gctctttacc ctggttgagg tagcgggttt tgcggtcggt
     5761 tttattatcg cagcccgcca ctagaataat tgcgctaagg cagaggctaa gggataaaga
     5821 agcacgcaaa cgcatagggc acctgtgaaa agtttaaatg ggcgaaaaat ctatatgggt
     5881 agataggctc agtggataga tggccttgat acacggctgt tgcaccatga gggctaagct
     5941 tagtcagtct gtgttgcaga gccgtgacaa tcgtgacaga accggcagaa agggtacggc
     6001 tgacctagat taagcgttgc aatgcaaaaa gcgttaaaat gcagctgata ctaaggcccg
     6061 cccgcaattg cacggtggag aatatatggc tatttagcac atattccatg gtgtaaaaca
     6121 gaccgattaa aaagctaatg ctgatggtga ggttgcgggt ttgcggatcg gccaccgggt
     6181 aaaaagccag tgtaagcgta ataaagatca ccaaataatc cgtcgggctg agttcaaagc
     6241 tgtgacggcg ggagaattgc aagccgatga tggtaaataa aaatataccc aagaatgcca
     6301 aaaatataag ggatttttgt gcggtaaagt ccacgggtat atggtaataa aagaatatag
     6361 ccgtggagca taatacatag atgcccatcc gctcgggcca ggccagggtg ggttggctgc
     6421 ggttgataaa acctgcgccg aggaataata gcgtcagccc aacaattaaa ataaataaat
     6481 cgctgttgcg cacggcggtg aagagcgcac tttgtaccaa tagggcacag agtaaaaacg
     6541 ctaaacaata acgccccaat aacaacaggc catctttgag ctggtggcgg gttggagtga
     6601 cggccatatg gggggcgagc aggcggccta agcgattagg gtgtcgatca caccaattta
     6661 aaccaccaaa taacacaatt aacacgctta ccaggattaa cagcagttca atatcggtat
     6721 gatagcgaaa ataaaaaccc agtagtgcca gtaagccctg caaaacatag atgagcacaa
     6781 cggtttcgta gtgatcaagg cccgcagcta acaagcggtg atgaatatga ttgcgatcag
     6841 gggaaaaagg cgagcggccc tgggctaagc ggcgggctat aacggcgcag gtatcaaaaa
     6901 tcggaatggc aaataacaat agcggaaata atgggctaat acctacatcg gcttgctggg
     6961 ttaagcgcag ggaggtaata ccggcgataa aacctaaaaa ttggctgccg gaatccccca
     7021 taaaaataat ggctggatga gtattaaagc gcaaaaaccc cattaatgcg cccaccacgc
     7081 ttaacgatat tagcgtgagc cctagatcac ccgcctgcat gctcaaaaat gcaattaggc
     7141 taaaacctac cagggttgag ccaccggcta aaccatccaa gccatctacc aaattaatgg
     7201 cattaataac gcccaccaaa accaccaggc tcagtatttg gctcattatt agaggcaatt
     7261 gcatatcggg caaaaagggc agtgtaacta tgcgtatatc cgcccccaga ataacaatag
     7321 cagcgcttaa acactggccg agcagtttaa ggcgataatc caaatccgcc cgatcatccc
     7381 aaataccaaa tgccagcagc acgattatgg ccaggaacaa gctgctatta ctgggctggg
     7441 cgggcagcca aataagcatt gggattaatg caccgcagac tatggcgatt ccccctgcgc
     7501 ggggaataag tttttggtgt actttgcgtt cattgggtgc gtcaattaat tttaagctgg
     7561 gcgagaactt tatcagaggc ggaataaagc tcaggctcaa cagcatggca acaatgaagc
     7621 aaataaataa aatcacgcgg gcgccttaat cgggaatatg gcgattcagc ttaggatagg
     7681 cgagggtaag aaaatcaatg gcccgttgat ccgcctgttc cagcgtggtg ttgcgatccg
     7741 tgacaaaact aatgcgcacc agagcgccat ctttgcgatg cagggtaatg gcatcccaca
     7801 tcaaatacca tttaactaaa aattcgttag tgaggtggcg atggcgctgg ttgaaaaaat
     7861 aatacaccat taatcgagta tcgccttttt gcacttgcaa gcgattaatc tcaaggtcca
     7921 ccccgggact ctttacattg ggtaaattaa tcgtagcggc gttttgaatt tcccagccgc
     7981 cggctggaat acaggagcgc ggcgagtgtg ccgattgccc tgcgctttgg gaattataaa
     8041 aagctacata caggttgata gcatcctggt ggttattttt ataatccacc gataaataat
     8101 catcaaactt aagttcgtct atgtagcgct gctctatggg cgcacgcacg ccggaccaat
     8161 gtcccaactc aagctcaaag gaagagaaag gagcgcgaga aattgttttt aaacccgcct
     8221 cgggtgtgaa ataagccacc gcggaaacca ccaaggccag cgcgcatgca atcacataac
     8281 tcaagggcaa ggtgcggttc tgttcggcta ctgcttgctt tactcgatcg gtgggcagtt
     8341 cgatcatgag caaatcaatt aggtgggcgc gaggtgtgga taaacgagca attaaagcca
     8401 tttcggcaaa cagaatggcg gtacatccca taaataccac ccagccttca aaatcgtgca
     8461 aaaacccctc ggccatggcc tgcccccacc attccacggt cacgccgata ataccgatgc
     8521 gcagactgtt cataagaacc gtaataggaa tggtggagag gaataccaaa atttttttcc
     8581 acagggggac tttaaaaaag tgtgcggcga taaagcctaa ggtcatcaga ggaaataaat
     8641 atctaaggcc tgagcaagct tcaaccacct gtaatttcat ggtgcctaaa tcaatgacat
     8701 tgccctctaa atagacggga atgttgaata gccggataaa atgcacccct atagaagacg
     8761 ataaaagctg tagttcaaac gatagatttt tgtaaatgaa gttgggtagc ggaacgctga
     8821 gtaaaagaat cagtagtggc actataaaaa atcgaaatgc ccgccagcca gttatggcaa
     8881 gcgttaatcc gcccagggaa atcaataatg agtactgcat taccgggaat aaggtgctca
     8941 tggcaccggc aaaacaccct agggtaccaa gtacaaatag ccccagcccc gcccaggaac
     9001 ccacaaaggg ctcagcggca atgtcgtgtt ttttctgcca gattaaaaac agagaaataa
     9061 acggcaataa aataccgtgg ctgtattcct ccttgtgatg ccaattaaac cagaggtttt
     9121 caaggcctgt tgcaaaaacc caggccagag ccgtgaaggc ggtagcaaaa ctcaaccaga
     9181 gggttaaggg tgaatgccaa taaacggtgg gggtgcggtg gtttggatgc ataaaactct
     9241 agggcaattg gcgtgaggca agggggctgg gtgaaaacat ttcgcggcga cgacgatcta
     9301 tgccttctaa aatatgcata aattgcagag ccaactggct gcgttcaaag gtgcgcgcaa
     9361 cataggccag gccattctcg ccgagtaatt cagcataggc gcgatggtca tggaggtgtt
     9421 caactgcgtc ggctaactgg ctggcatctt caggggtaat acaaatgccg gattctgatt
     9481 cattaataat atcggcaacc tcaccttcta cacccaaaac tatgggccgt ttcatcgcca
     9541 tgctttcaaa aattttggag ggaattactg ttttaaataa atcagatttc ttgagcagta
     9601 ccaggctggt atccaccaat tgccagaggc ggggcatgtc ggctttgggc agttgatcca
     9661 gcataataac attatgcagc gacttttcct gcttaatttt gactaatttg gccttttctg
     9721 aaccgtcacc ggccattaaa aagcgaattg atggatggtc tttaagaatc tcagcggctt
     9781 ctaaaatagt ttctaaatga tgggccatac catgagtgcc tacataagcg gcaaaaaaca
     9841 tggagggttc atagtgcacg ccaaaatact gagctgcgtc ggctttagat tgcacctcgg
     9901 cgcagtcaaa aagacttaaa tcaacaccgt tataaattac cgacaattta tcagccttgc
     9961 cgcctcgggc caggatatgt
//
LOCUS       DAOQGS010000083         9979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3152_length_9979_cov_3.964027, whole genome shotgun sequence.
ACCESSION   DAOQGS010000083 DAOQGS010000000
VERSION     DAOQGS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_9979_cov_3.964027"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PKZ52_04725"
     CDS             <1..707
                     /locus_tag="PKZ52_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNG59107.1"
                     /translation="CNAGDHGQPFATNFNPEINIREVTAKGRYWENGKWVEIDAMSIK
                     DVYNFPAGIGEKDIYLLYHEELESIVKHFPEIKRARFWMTFGQSYLKHLEVLQNIGMT
                     SITPVMYNGVEVIPLQFLKSVLPDPSSLGPLTKGKTCIGCVAKGIKDGQEKIIYIYNI
                     CDHEECYAEVQSQAISYTTGVPAMIGAKMMLEGKWKKPGVWNMEQFDPDPFMADLNSY
                     GLPWEVVELKTTGLNV"
     gene            710..1882
                     /gene="nspC"
                     /locus_tag="PKZ52_04730"
     CDS             710..1882
                     /gene="nspC"
                     /locus_tag="PKZ52_04730"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNG59108.1"
                     /translation="MANPLNTLVNFDLNRVPSPCYVVDEAAIEHNLQILHRVQEESGA
                     KVLLALKAFSMFAVSHLVKKYLTGTCASGLFEARLGKEEFGGEVHTYAAAYRPEDMEA
                     ILAVSDHVVFNSFTQWQRYKEQALNAQKTNPKLSFGVRINPEHSEGAVAMYDPCAPCS
                     RMGIPIAAFEGQDLTGLDGLHFHTLCEQDFEPLRRTLDVIETKFAHILPTLKWINFGG
                     GHHITRADYQVDALIARIKNFQQKYAVQVYLEPGEAIALNSGIFVAEVLDTSWNGMNL
                     AILDASATCHMPDVLEMPYRPRIPGSQEPGVLPHTYRLGGPSCLSGDVIGDYSFAKPL
                     QVGDRLMFEDMAIYTMVKNSTFNGIPLPAIALYNSVTDELRIVKQFGYEDFRERLS"
     gene            2249..3178
                     /locus_tag="PKZ52_04735"
     CDS             2249..3178
                     /locus_tag="PKZ52_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNG59109.1"
                     /translation="MHKKITDLSPAELENLSFDLLLKLGIKNLVWRTPGRDGGRDLQG
                     EFFIEDISGFTNRQSWYVDCKRYNTSVSWPVVWEKISYAESNSADALLIVTTTSLSPQ
                     AVDEVNKWNEKRNRLAIRFWNSVDLESRLKPYPEISIKYGLANNPEESKGLAILPLTR
                     ILLKYSNSAHSCHVFGYGQGADRLNVVYSISELIANRLNDFDRDNSLSIYPYKSKEDS
                     FDWIRGDEIIETLGFDKYAFRALSSYVYEITPGNELRISERNGSAYIEVGQSIPANTQ
                     EDIIDISLLSNFQISFEELGIILTKSKVLHAQK"
     gene            3165..3893
                     /locus_tag="PKZ52_04740"
     CDS             3165..3893
                     /locus_tag="PKZ52_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNG59110.1"
                     /translation="MHKNDQTKHLIANITHQAINPLNGVLGTIDNIIDGTISDDRRNQ
                     RLGSARAQLEYTITLIRNLAYFAEYGGEEPQTKKNSMKTTVIPQNLIEGAMFFQEQAD
                     NKKMKIRVENKHIQNAIKGDPDLLRQVFMNIVDNYIKYGREGCTVIVNHWIQRKTNDI
                     IIKFEGESIPFSSDENIFGLGVRGKDAEGETSSGSGLGLHICKLIVEKIFGGKISAEY
                     ISGRKSSVFEIRIPNGFITEGRRS"
     gene            3890..4327
                     /locus_tag="PKZ52_04745"
     CDS             3890..4327
                     /locus_tag="PKZ52_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG59111.1"
                     /translation="MIELSRHVLIMDDEPHLLDWLEEYLESKEYEVHQVTNVQDAISK
                     LDINIYRMVVLDLNVPASAEYLEKLKAKGDLYVEYRGLYVAEMARTKGHRGRQVVVYS
                     VHDSDKASLVCNKIGVTYLVKGRPRQFKKELDDILSYAPQQNT"
     gene            4569..5003
                     /locus_tag="PKZ52_04750"
     CDS             4569..5003
                     /locus_tag="PKZ52_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59112.1"
                     /translation="MPISNLSIYHNIATESYSNLKFRIEKEKISKEGGSNGHAINYDP
                     DLNTFRQSMITIVFAGMWLEQNIYEEILSRTGLDIVRRYNSKEYREWLIRLEIVSAEV
                     LDEIDAFMSAREILMNSTIVNDKSIETAQKEASRAQRIIASI"
     gene            5196..5552
                     /locus_tag="PKZ52_04755"
     CDS             5196..5552
                     /locus_tag="PKZ52_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59113.1"
                     /translation="MSNKPLWIISSKARPLEGCPIKFDNSEFYFVEGLVPANSTSEAQ
                     APLAHALRDEMFELAEVLSCKPYVASEWEDTEQFPEIEEAARKSAQTGNLAFALFISE
                     EPKENHDPDDDDVDWS"
     gene            5565..6263
                     /locus_tag="PKZ52_04760"
     CDS             5565..6263
                     /locus_tag="PKZ52_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59114.1"
                     /translation="MDINTTAAMSMAEGHPRYAMDVSMVFGGKTERDRVLRTTFDQLG
                     VWKTKYADVGISKYEPEKYVTFKEAATIINPATKKEEIWVFGIDSTKADDIVTAVEIA
                     TDKYKTPPGDFLRNIYIKNLNAEREHEIRHQGGLQALVNANKKLYTGVSDAIREAARK
                     LRITGELNLWVVSSNVNEKIPKTDLHNALREGGAEAVGVAKKPFDMRSGANEGKGAAA
                     LIKGTNLHLAKFRV"
     gene            6659..7222
                     /locus_tag="PKZ52_04765"
     CDS             6659..7222
                     /locus_tag="PKZ52_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59115.1"
                     /translation="MIETKNNTALSPVFLHYLIKSTSCVCLTIALISPQYVSASESPK
                     LLIDGFGEAEFQPEYSGPTQDGEPYVARRWWRRTNGDVIVSVAEFDFKKPRSPKSWLR
                     YWKTSHACVAQPVKDVKSLPDLGTQLTIAGVCKGGDSFAMHILHIDSTIVELNVASMD
                     YRRSQSELIQLLGSFLSRVHSKPASEK"
     gene            8202..8801
                     /locus_tag="PKZ52_04770"
     CDS             8202..8801
                     /locus_tag="PKZ52_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HNG59116.1"
                     /translation="MYDQYDALIFDMDGTLVDSGQLHEFGWTTMLNKYAIPIDRQLMR
                     SLAGVPTKGTIEILLEKFNLKRAASLDEMNAFKEQVVHENMHKYVKPTALLGVAQKYH
                     GIKPMAVGTGAYTQEAAAILELCGLYKYVSVVVGADQVINPKPAPDTFLRCAELLGVD
                     PKQCVVFEDSKLGLQAAASAGMVGIDVLLVHAITNEYFV"
     gene            complement(8872..9120)
                     /locus_tag="PKZ52_04775"
     CDS             complement(8872..9120)
                     /locus_tag="PKZ52_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59117.1"
                     /translation="MATFNGIPCNFGAAAALAPKRYGIRCISSDPIRQLRFFKGTCQF
                     EPEEFLRVTVLSPSEYRKQQQSGSFAVNENLQSGSPIG"
     gene            complement(9123..9320)
                     /locus_tag="PKZ52_04780"
     CDS             complement(9123..9320)
                     /locus_tag="PKZ52_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG59118.1"
                     /translation="MRNNLKDLRSARGWSQAELAEALQVSRQTINAIETGKYDPSLPL
                     AFKIAAIFGLAIEGIFFPVDL"
     gene            complement(9310..9708)
                     /locus_tag="PKZ52_04785"
     CDS             complement(9310..9708)
                     /locus_tag="PKZ52_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59119.1"
                     /translation="MGGSKQYVKEFAWVMGTYGLLLPLCLWRIYNGASGIGKYAWAIL
                     PTLPLLALIAVIAREIARMDEFMQRVHVLSFGITLAITGAVTTLWGFLEIAGLPPISI
                     IYVFPFSVSTWGIINCLMFRHYASVNADAQ"
BASE COUNT         2983 a         2237 c         2241 g         2518 t
ORIGIN      
        1 actgcaacgc cggcgaccac ggccaaccct ttgccaccaa cttcaacccg gaaattaata
       61 tccgcgaagt aaccgccaaa ggccgctact gggaaaacgg caagtgggta gaaattgatg
      121 ccatgtccat caaagatgtg tacaacttcc ccgccggaat tggcgagaaa gatatttatt
      181 tgttgtacca cgaggaactc gaatccatcg ttaaacactt cccggagatc aaacgcgcac
      241 gcttttggat gaccttcggc cagagttatt taaaacacct agaagtgctg caaaacatcg
      301 gcatgaccag cattacgccg gttatgtaca acggggtgga agttatcccg ctgcaatttt
      361 taaaatccgt attgcccgac ccttccagcc tcgggccttt aaccaaaggc aaaacctgca
      421 tcggctgtgt tgccaagggt attaaagacg gccaagaaaa aattatttat atctacaaca
      481 tttgtgacca cgaagagtgt tacgccgagg tgcaatccca ggctatttcc tacaccaccg
      541 gcgtgcctgc catgattggc gcgaagatga tgttagaagg caaatggaaa aaacccggtg
      601 tatggaacat ggaacaattt gacccagatc catttatggc agatttaaat agctatggcc
      661 taccctggga agtggtggaa ttaaaaacca caggattaaa cgtttaatta tggccaaccc
      721 gttaaatacc ctggttaatt ttgatttaaa ccgcgtaccc agcccctgtt atgtggtgga
      781 tgaagctgca atagaacaca atttgcaaat tcttcaccgc gtgcaagaag agagcggcgc
      841 caaagtatta ttggctttaa aagccttttc tatgtttgcc gtcagccact tggtaaaaaa
      901 gtatttaacg ggcacctgcg ccagcggctt atttgaagcg cgcctgggta aagaagaatt
      961 cggcggcgaa gtacacacct acgccgccgc ctaccgcccg gaggatatgg aggcgattct
     1021 cgccgtatcc gaccatgtgg tatttaattc ttttacccag tggcagcgct ataaagaaca
     1081 agccttaaac gcgcaaaaaa ccaaccctaa attatccttt ggtgtgcgta ttaaccccga
     1141 gcactccgaa ggcgcagtcg ccatgtacga cccctgcgcg ccttgctccc gcatgggtat
     1201 tcccatcgcc gcatttgaag gtcaagacct aaccggttta gatggcctgc attttcacac
     1261 cctgtgtgag caagacttcg aacccctgcg ccgcaccctg gatgtaatcg aaactaaatt
     1321 tgcgcatatt ttgccaacac taaaatggat taatttcggc ggcggccacc acatcacccg
     1381 cgcggattac caagtagacg ccttaattgc gcgcattaaa aatttccaac aaaaatacgc
     1441 cgtacaggtg tatttagaac ccggcgaagc catagcgtta aatagcggta tttttgtggc
     1501 cgaagtgtta gacaccagct ggaacggcat gaacctagcc attttagatg cctccgccac
     1561 ctgccacatg cccgacgtat tagaaatgcc ctaccgcccg cgcatacccg gcagccagga
     1621 gccgggtgta ttaccccaca cctatcgttt aggcgggccg agttgtttat ccggggatgt
     1681 gattggcgac tatagctttg ccaaacccct gcaagtgggt gacaggttaa tgtttgaaga
     1741 catggccatt tacaccatgg taaagaacag cacctttaat ggcattcccc tgccggcgat
     1801 tgctttgtat aactcggtta ccgatgagct gaggattgtg aagcagtttg ggtatgagga
     1861 ttttagggag aggctttctt aatttttgga atgggagttg ctgtaaaaaa accgcctttt
     1921 agggcggttt tttttgggga agtggatttg ggttgggtgc ttgttcgagg tgggcatttg
     1981 acgtataaag accaagaatc actttagttt tgcgtcatca ccacttctga ccctagcgga
     2041 tcaaagcgag tgtccgttgc aaatacgatc gcagagcact gtaattgttt gaaaagcaat
     2101 acctaccata ttttgaaaac tttattgcat agagtttggt gactttatta ccaatggatg
     2161 gttttcggtg aatttaccag ttaggcagtg cgacaaacgg cagacaacgt cgattaaaac
     2221 atttacattc cagagaaaca cagtcgaaat gcataaaaaa ataacggact tatcccctgc
     2281 agagctagaa aatttaagtt tcgacctttt gttgaaactc gggataaaaa atttagtgtg
     2341 gaggacacct ggaagagatg gaggtcgaga tcttcaaggc gagtttttta tcgaagatat
     2401 ttctggcttt accaatcgcc aaagctggta tgtcgattgt aaacggtaca acacctctgt
     2461 cagctggcct gtggtatggg aaaagattag ctacgccgaa tcaaactcag cagacgctct
     2521 attgattgtt acaacaacct ctttatctcc acaggctgta gatgaagtaa ataaatggaa
     2581 cgaaaagaga aatagactgg caattcgatt ttggaactcg gttgacctgg aatctcgact
     2641 aaagccatac ccagaaatat caattaaata tggtcttgct aacaacccag aagaatcgaa
     2701 aggtttagcc attcttcctt tgactagaat actattgaag tattctaact cagctcactc
     2761 ttgccatgtg tttggatatg gtcaaggagc agacagatta aatgtcgtat attcaatttc
     2821 agagctaatt gccaacagat taaatgactt cgatagagat aattctcttt cgatttatcc
     2881 ttacaaatcc aaagaagatt catttgactg gattagaggt gatgaaataa ttgaaactct
     2941 cggtttcgat aaatatgcct ttagggcatt atcatcctac gtttacgaaa tcactccggg
     3001 aaatgaatta agaatatccg aacgaaatgg ttcggcttac attgaagtag gacaatccat
     3061 accagccaat acacaagaag atataatcga catatctctc ctttccaatt ttcaaatttc
     3121 tttcgaagag ctcggaatta ttctgacgaa gtcaaaggtt ctgcatgcac aaaaatgacc
     3181 aaacgaaaca tttaatagca aacattactc accaagcgat taacccccta aatggtgtac
     3241 taggaacaat agacaacata attgacggaa ccatttctga cgacagaaga aatcagaggc
     3301 ttggcagtgc aagagcccaa ctggaatata cgattacttt aattcgcaat cttgcttatt
     3361 tcgccgagta cggcggggaa gagcctcaga ccaaaaaaaa ctcaatgaaa acgacggtaa
     3421 ttccccagaa cctcatcgaa ggtgccatgt tcttccaaga gcaagccgac aacaaaaaaa
     3481 tgaaaattcg cgtggaaaat aagcatattc agaacgcgat taaaggcgac cccgaccttc
     3541 ttaggcaggt ctttatgaat attgttgata attatattaa atatggaaga gaaggctgca
     3601 cagttatagt taatcattgg attcaaagaa agactaatga catcataatt aaatttgagg
     3661 gcgaaagcat cccatttagt tcggacgaga atatttttgg ccttggagta agaggaaaag
     3721 atgcagaagg agaaacgtct tcaggctcag gactggggct acatatttgc aagttaattg
     3781 ttgagaagat atttggcgga aaaattagtg ctgagtatat atccggccga aaatcttctg
     3841 tatttgaaat tcgaatccca aatggcttta ttactgaggg tcgccgttca tgatagagct
     3901 ttcaagacat gtattgatta tggatgatga gccacacctt ttagactggc tagaagagta
     3961 tttagaaagc aaagaatacg aagtgcatca agtgactaat gtacaggatg ccatttcaaa
     4021 acttgatata aatatatatc gcatggtcgt gctggatctg aacgttcctg catcagcgga
     4081 gtatcttgaa aaattgaaag ccaaaggcga tctttatgta gagtatcgcg ggctttatgt
     4141 tgcagagatg gctagaacaa aaggacatag aggacgccaa gttgttgtgt attcggttca
     4201 tgactccgat aaagcctcct tggtatgtaa taaaattggg gttacatact tggtaaaagg
     4261 caggcctcgc cagttcaaaa aagagctcga tgacattcta agttatgccc cgcaacaaaa
     4321 cacatagact tgcggatcaa gggttagaga ccttgaaaaa ataactgaaa aaccaactca
     4381 tcgataccac taaaatatca tagataaata atacggaacg cgtaacccgt acaaaccatt
     4441 ttcgtagggc gcaccttggt gcgccgaaac attgagatgc gctgactcca aatggcgtac
     4501 cgaggtacgc cctacgagct atcactttat agtcaaatcg tagttcaccg cttgcccaag
     4561 agaaaaatat gccaatctca aatttatcga tttaccataa catagctaca gaatcgtact
     4621 cgaacctgaa attcaggata gaaaaagaga aaatttctaa ggaaggagga tcgaatggac
     4681 atgcaattaa ttatgacccc gacttaaata cttttaggca atcaatgata actattgtgt
     4741 ttgcaggaat gtggcttgag cagaatattt atgaggagat tttatcgaga acaggccttg
     4801 atatcgtaag gagatataat tcaaaagagt atcgagaatg gcttattcgt cttgaaattg
     4861 tcagcgcgga ggtattggat gaaatagatg cgtttatgtc ggccagagag attttaatga
     4921 attcaacaat tgtcaacgat aaatcaattg aaaccgctca aaaagaagca tctcgtgccc
     4981 aaagaattat agcgagcatc taaaatgtat tggtcttgaa gtccacgcgg cgatagctcg
     5041 taggttgggc agtaatgccc aacatttgta atagacctat gaagccaaat catatttacg
     5101 acaatgtgtt gggcattact gcccaaccta cgagctgccc gggcccctat aagataaatt
     5161 tgcaacgttt acatcaaaca taatgaggag tgtgtatgag caacaaaccc ctatggatta
     5221 tttccagcaa ggcacggcct ttagaaggct gtccgataaa atttgataac tccgagtttt
     5281 attttgttga aggcttagta cccgcaaact caacaagcga agctcaagca cctttagctc
     5341 atgcactgcg cgatgagatg tttgaattag cagaagtcct atcctgcaag ccttatgttg
     5401 ccagcgagtg ggaggataca gagcaattcc ccgagatcga ggaggcagca cgcaaatcgg
     5461 ctcaaacagg taatttggca tttgccctgt tcatatccga agagccaaaa gagaaccatg
     5521 acccagatga cgatgacgtg gactggtcgt aaggaggctt atttatggat atcaacacta
     5581 ctgcagcaat gagcatggcc gaaggccacc ccagatacgc catggatgtg tctatggtat
     5641 ttggcggcaa aactgaaaga gaccgagttt taaggaccac atttgaccaa ctgggcgttt
     5701 ggaaaaccaa atacgcagac gtaggcatct cgaaatacga acccgaaaaa tatgtgacgt
     5761 ttaaagaggc cgccacaata atcaacccag cgactaaaaa agaagagatc tgggtgtttg
     5821 gaatagattc aacaaaagcc gacgatattg taaccgcagt ggaaattgcc accgataaat
     5881 acaaaacccc tccaggcgat tttttgcgga atatctatat taagaatctc aatgccgagc
     5941 gagaacacga aattcgccac caaggcggtt tacaggcatt agttaatgcc aataaaaaac
     6001 tttacaccgg tgtttcagac gctattcgcg aagctgcacg caaacttcgc ataactggtg
     6061 agctgaatct gtgggtggtt agcagcaatg taaacgaaaa aatccccaaa acagaccttc
     6121 acaacgcgct tagggaaggc ggcgccgaag cggtaggtgt tgccaaaaaa ccttttgata
     6181 tgcgcagtgg tgcaaatgag ggcaaggggg cggcggcact tatcaagggt acaaatttac
     6241 atttggcgaa attccgcgtc taaaaattaa ttcccgagat cgcaacgtag cttgctgggc
     6301 ggaaatgccc ggcatttaca atcaagctcg tagataccat ctctccccat actgccgcaa
     6361 gcattaattc cagttgctag cggcagtatg gggagagatg gtatctacga gcttaacgcg
     6421 caacctgatc gaggagtcag aacgcgtaac cagtacaacc cattttcggg ggggcgcacc
     6481 ttggtgcgcc gaaacattga gatacgctga ctccaaatgg cgtaccaagg tacgccctac
     6541 gaggaacata aggccaaaaa ttattccaag ttacgcgttt aaataagtat catctagttg
     6601 ggcaccactt gcatcgggag tggaccaaaa ttctgaggct aacaactcga gcaattgcat
     6661 gattgagact aaaaataaca ccgccttatc accagtattc ctgcattatt taatcaaatc
     6721 caccagctgc gtgtgcctga ccatcgcgct aatcagcccc caatacgtat ctgcaagtga
     6781 aagtcctaag ctgcttatcg atgggttcgg tgaagccgag tttcagcctg aatactcggg
     6841 cccaacccag gacggagaac cctatgtggc acgtaggtgg tggcgccgca caaacggtga
     6901 cgtgatcgtc tcagtcgccg aatttgattt taaaaaacct agatcaccca aatcctggct
     6961 tcgatattgg aaaacctctc acgcctgtgt tgcacagccg gtaaaggacg tgaaatcttt
     7021 acctgaccta gggacacaac taaccatagc tggagtttgc aaagggggcg attcattcgc
     7081 aatgcatata ttgcacattg actccaccat tgtagaacta aatgtagcct cgatggatta
     7141 caggcgttca caaagcgaac ttatccaatt gctgggcagc ttcttgtcgc gtgtacattc
     7201 taaacctgcc tctgaaaaat aactcgtaag gtgggttcgc gaagcaaccc accatcagta
     7261 acatacgcag tgatcaaggg gtcagtgagg ggtgttcaaa ttctgtgtca ccgagtatta
     7321 agacgctaac gcgcttcgct tgttgcctcc ccgaaagtca cagtgggacc tgctccttcg
     7381 ccaatcaccg aaatagataa attaattttt ggtggtatgt aaaaataaat attgacatcg
     7441 agtgggaatc acttaaaaat taccgaagcc agctgagctt cgaccagcat ttactcgcaa
     7501 ctacccttaa gggtattgca ttttaattta gccatcagct tttctagatg gccatactaa
     7561 gcgacagcct tttagagctg ttgtatttcg atgaagccat attttcatta tttttaaatt
     7621 atggctccta ccaagcgaca gtctttctag ctgttgtatt ttaatgcaac catattctta
     7681 tacttctaat tttatggctt ctacgacgcc acatcctacc ccgtaggtgt tgccttttgc
     7741 ctagtcggcg gtgctttttc ggcggcgacg actcattaac gaagctcctt tttacgttgt
     7801 taaaagggag ccgcatagat ggtaacgggg tcccatcttt ccaatgcatt gtacagtcat
     7861 tttagatcac tcagaccctt caccatttga ttggagcgcc atgtggaatc tttaatcaaa
     7921 gtcatcacct tagcaacaca aattccagac acaaagttag cgttaattat tgcgttcact
     7981 agcgtggtag gcatttggtc gatagtgatg tttagaaaaa gataaatatt cttaaaaaaa
     8041 ggggggcgcc ttatagcgct ctcttgtctc acctttggtt acacattcaa gcacttcact
     8101 ataattaata gcgatttgat acacgtcata tcccacctat tccgccctac gggctgatcc
     8161 aaccgaggag ttgaattaac cggcaacaat gagagcacaa gatgtacgac caatacgatg
     8221 cactaatttt tgatatggac ggcaccctgg tggatagcgg ccaactacat gaatttggct
     8281 ggactacaat gctgaacaaa tacgcgatcc ctattgaccg ccagttgatg cgttcattgg
     8341 cgggtgtgcc caccaaaggc accattgaaa ttctgctgga aaaatttaat ctcaagcgtg
     8401 ctgccagcct tgatgaaatg aatgcgttta aagagcaggt tgtgcacgag aatatgcaca
     8461 aatatgtaaa gcctactgct ctgctgggag ttgcacagaa ataccacggc ataaaaccta
     8521 tggcagtcgg aacgggtgcc tacactcagg aagccgcagc cattttagaa ctgtgcggct
     8581 tgtataaata cgtaagcgtt gttgtgggcg ctgatcaggt aataaatcca aagcccgctc
     8641 ccgatacctt tttacgctgc gcagagttat taggcgttga ccccaaacaa tgcgtagttt
     8701 ttgaggattc aaaactcggc ttacaagcag ccgctagcgc aggcatggtt ggtatcgacg
     8761 tgttgttggt acatgcgata actaacgagt atttcgtgta aggcgcagct ggcacaatcc
     8821 cagcacttca gtgcgccctt tgagtgtgac gtcgccacgc ggaatcattt attaaccaat
     8881 tggcgaaccg ctttgcaaat tctcattaac cgcaaaactt ccagattgct gttgttttcg
     8941 gtattcgctg gggcttaata cggtcacgcg caggaattcc tcaggttcaa actgacaggt
     9001 acccttgaaa aatcgtaact gccggatcgg atctgagcta atacacctta tgccatagcg
     9061 ttttggcgcc aaggctgccg ccgcgccaaa attgcaaggg atgccattga atgttgccac
     9121 gcttataaat cgacagggaa aaaaatccct tcaatggcca ggccaaaaat tgccgcaatt
     9181 ttaaaggcca aaggcaagct tggatcatac ttgccggttt ctatggcatt gatggtttgc
     9241 cgcgatacct gtaaagcctc cgccaattcc gcctgcgacc agccgcgtgc gctgcgcaaa
     9301 tcttttagat tattgcgcat cggcattgac gctcgcgtag tgcctaaaca tcaggcaatt
     9361 aatgatcccc caagtgctta ccgaaaaggg aaatacataa ataatcgaaa tcggtggcaa
     9421 accggcgatt tctaaaaacc cccatagtgt ggttacggcg ccggtaatag ctagagttat
     9481 accaaaactc agcacgtgaa cccgctgcat aaattcatcc atacgggcaa tttcccgcgc
     9541 aattacggcg attaatgcca gtagcggcaa ggtaggcaat attgcccagg catatttgcc
     9601 aataccgctt gccccgttgt aaatacgcca taaacacagc ggcagcaata ggccataagt
     9661 acccattacc cacgcaaatt cttttacata ttgtttcgag ccgcccatac tgcctccaag
     9721 tcaagtttac tttacatcaa tttagccggt gtgccgatgc ttgtcaagct tactttacac
     9781 aaagagttcg caactaggca gtcaatgcag ccagcagctc tgccaatggc tgttgaataa
     9841 atatcaattt gtgaccattc agcacacatt attaccctcg ccacacgggt ggcgaattga
     9901 ggggagattt attaacccgg cggcaattag tgccgggtta ataaaggcgg caaggatgcc
     9961 gcgtcaaagg tgcaaaccg
//
LOCUS       DAOQGS010000084         9908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3187_length_9908_cov_5.446666, whole genome shotgun sequence.
ACCESSION   DAOQGS010000084 DAOQGS010000000
VERSION     DAOQGS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9908
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3187_length_9908_cov_5.446666"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1138)
                     /locus_tag="PKZ52_04790"
     CDS             complement(317..1138)
                     /locus_tag="PKZ52_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HNG59120.1"
                     /translation="MPLGAVSQAIQLLPLIVLGLIAFGFYLRRQIICRQQLQGVRWLQ
                     AMRILLAHIQKHRGLSAGVMSGDRSLLPQLEEVQQLISRDLLHIADVGDWVKDNVNWL
                     AVTAHWARLAGRLHSMGLLNAIDQHNRLIKNILVFIDDIAAEHYLTKDVRGAPNNWRE
                     LLAAAESVGQARALGTALTAGGRYFEFALKTRAELIQVMLQLQEMLEKTDLPLAQQQA
                     VNELLNYIKTHILVNEQSSAADFFARATTCLDSILLAFDTELSQVQQRLSNVRST"
     gene            complement(1560..2957)
                     /gene="thrC"
                     /locus_tag="PKZ52_04795"
     CDS             complement(1560..2957)
                     /gene="thrC"
                     /locus_tag="PKZ52_04795"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNG59121.1"
                     /translation="MNYISTRGKAPKLSFEDVVLTGLASDGGLYVPESIPQFSQTEIA
                     SFRSLSYQELAYKIIAPFVDGEIDEANLKAIIAKSYAQFRHGAIAPLVQIGQNEWILE
                     LFQGPTLAFKDFALQFLGNLLDFILAKRQQKVVVMGATSGDTGSAAIEGCRYCENVDI
                     FILHPHNRVSDVQRRQMTTVLAPNVHNIALQGNFDDCQNMVKASFSDQSFLPAGRQLV
                     AVNSINWARIMAQIVYYFYAALALGAPDRKVSFSVPTGNFGDIFAGYLAKRMGLPIDQ
                     LIIATNSNDILHRCISANDHSKKPLIHSLSPSMDIMVSSNFERMLFELYNRDGAAISQ
                     LMDDFKAGSMSLSQEVLASAQELFASYKLDDAEMLSVVAEVFNSSEYLLDPHTAIGVQ
                     AARKTRRDLSVPMVCLATAHPAKFPEAIEKSGVGVVPQLPHYMQDLFSREERCAVLPD
                     DIKAVQGFMQARLGV"
     gene            complement(2985..4289)
                     /locus_tag="PKZ52_04800"
     CDS             complement(2985..4289)
                     /locus_tag="PKZ52_04800"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNG59122.1"
                     /translation="MKTVRIGICGLGTVGSGTFNVIQRNGQEINARAGCEIRIVQVGC
                     RKDNPSCDTHDVAVTRDIFEVAKNPDVDILVELIGGTTTAKTLIETAIAHGKHVVTAN
                     KALIAQHGNELFAMARAKGVSIAFEASVAGGIPIIRILKEGLSANRIEWLAGIINGTG
                     NFILTEMKDKGRSFESVLAEAQQLGYAEADPTYDVEGIDAAHKLAILGSLAFGMPLQF
                     TRVYTEGISKIAPVDVSYAHELGYRIKHLGIARKHADNRVELRVHPTFIPEKRLIASV
                     DGVSNAVVVKGDAVGSTLYCGPGAGAEPTGSAVISDIIELSRTLLAPPEYRAPYLGFQ
                     DDQLNSFSILDIEEIETSYYLRMSAADQPGVLSKITQILSDAGISIEALIQKQPKDDD
                     TQVPVILLTNKVLEKNLNRAISLIEALDTVVGPVVRIRVESL"
     gene            complement(4483..5298)
                     /locus_tag="PKZ52_04805"
     CDS             complement(4483..5298)
                     /locus_tag="PKZ52_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNG59123.1"
                     /translation="MKLTSSLLTGAICLALAAGAMAADKPKQVELPIGVTGQNAAAPA
                     NEGSLEEIKQRINKTLQGAKPDLSVSDVQRSSIPGVYKVSLGQGPAIYTNADGTSFIV
                     GDFYRINGAEVVNVSDEERNGLRAKAVAEIKKDDMIIFSPEGKPKASIYVFTDVDCGY
                     CQMLHKNVGKMNELGIEVRYLGYPRAGLNTASFNKLASAWCAADKKAALTKLKNREEI
                     PNNVCPNNPIAAQFQLGQQLGVSGTPALVFENGQMVPGFVEPERLAQMLNIKG"
     gene            complement(5550..5912)
                     /gene="rplS"
                     /locus_tag="PKZ52_04810"
     CDS             complement(5550..5912)
                     /gene="rplS"
                     /locus_tag="PKZ52_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNG59124.1"
                     /translation="MSSKNKIIQELENEQLKTDLPSFAPGDTVVVHVRVIEGERERLQ
                     AYEGVVIAKKNRGLNSAFTVRKISHGVGVERTFQTHSPQIGSIEVKRRGDVRQAKLYY
                     LRDLAGKAARIKEKLTSK"
     gene            complement(5962..6720)
                     /gene="trmD"
                     /locus_tag="PKZ52_04815"
     CDS             complement(5962..6720)
                     /gene="trmD"
                     /locus_tag="PKZ52_04815"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNG59125.1"
                     /translation="MNIAVLSLFPEMFSAFANLGVTGRAVKQGLIKLHCISPRDFAFD
                     RHQTVDDRPFGGGPGMVMMVEPLRKALQQAKDMLAASGALQSKVIYLSPQGKRFDQSA
                     AERLSTEPALIFVAGRYEGVDERFVQRYVDEEWSIGDYVLSGGELPAMVMVDAMARLL
                     PGVLGAAESAVQDSFVSGLLDCPAYTRPEEYDGDRVPEVLVSGDHKKIANWRLQQSLG
                     RTWERRPDLLEKLELSPEQLKLLAQYQAGRKSDT"
     gene            complement(6767..7294)
                     /gene="rimM"
                     /locus_tag="PKZ52_04820"
     CDS             complement(6767..7294)
                     /gene="rimM"
                     /locus_tag="PKZ52_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209063.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNG59126.1"
                     /translation="MNSKLVVGQIVGVFGIKGWLKVKSFTEPADNLLHYAPWYLGLPQ
                     GLKQFAIQDYAQRPQGAVVKLKGIDDRDQALALGKASIYVERDQLAELAVGEYYWSQL
                     IGLRVVSLFGGNEQDLGVVHSLLETGSNDVLVVRGDGTSIDQGERLIPYLPGQFVIEV
                     NLALGIIRVDWDPEF"
     gene            complement(7337..7588)
                     /gene="rpsP"
                     /locus_tag="PKZ52_04825"
     CDS             complement(7337..7588)
                     /gene="rpsP"
                     /locus_tag="PKZ52_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNG59127.1"
                     /translation="MVSIRLARGGSKKRPFYHVTVTHSEKALGGSFIERLGFFNPVAR
                     GKEERLRIDLERLDFWVKNGAQCSDRVAKLAKDAAVAAA"
     gene            complement(7730..9100)
                     /gene="ffh"
                     /locus_tag="PKZ52_04830"
     CDS             complement(7730..9100)
                     /gene="ffh"
                     /locus_tag="PKZ52_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNG59128.1"
                     /translation="MFESLSDRLTRSLRKITGKARLTHDNIQDTLREVRKALLEADVA
                     LSVVKDFINEVRKRAIGQEVSRALNPGQQFLKIVEQELTQMMGEANEKLNLAMQPPAV
                     VLMAGLQGAGKTTSVAKLARFLKDKEKKKVLVVSADVYRPAAIKQLETLAAEVSAEFF
                     PSSSDQNPIDIARNAIAHGRKIFADVVIVDTAGRLHIDEALMDEIKALHAAINPIETL
                     FVVDAMIGQDAVNTAKAFNDALPLTGVVLTKADGDARGGAALSVRHITGKPIKFLGVG
                     EKVDALEPFYPDRLASRILGMGDVLSLIEDVEQKLDKAKAEKLIQKVKKGKSFDLEDL
                     RDQLQQMQNLGGMGAILGKLPGMGDITAAAEKADVGKQFKQMEAIISSMTPGERRDPE
                     LINNSRKRRIAMGSGTDIQDINRLLKQHKQMAKMMKKFKGGGMSKMLRGIGGMMGGGT
                     PPGLGR"
     gene            9333..>9908
                     /gene="ccsA"
                     /locus_tag="PKZ52_04835"
     CDS             9333..>9908
                     /gene="ccsA"
                     /locus_tag="PKZ52_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNG59129.1"
                     /translation="MRRLTQRQPLSSGLISGFICLALSLNAWAIFEQIHVATGYQFGF
                     FKVAPLFFWAANLLIFLSSLKRPLHSLLLFTLPMSLTASLVSLLVSNSSATPIALGPG
                     LLTHVLLSLLAYSLMLAALVQALLLAYQNQKLRSKHPGGWLRLLPPLQTMEALLFELL
                     WAGELVLTLAIISGGLFVENLLAQHLAQKTVF"
BASE COUNT         2472 a         2743 c         2328 g         2365 t
ORIGIN      
        1 gaaaagcccc attctaactc attctagcca gcggaattct cacgggccgg ggattaaaaa
       61 cgtctcggca taactattaa cagcctgttg gaatacgcca gctgcgacga gtaattaagg
      121 attttttgcc gaaatttaac gccgccagaa gtagcgaaat agtgtgtcag cgggctgccc
      181 ctgcgtcagc ctgctaggcg acacgaatcg gcaagactca ttcagcagag atttctcgcc
      241 gctcagtatt aaaacctaat gagaccgcat cctattggca attgaagcgc agaataaaaa
      301 cccatgggca ggcgggttaa gtactgcgca catttgataa gcgctgctga acttggctaa
      361 gctctgtatc gaaagccaat aaaatactat ccaaacaggt tgtggcacgg gcaaaaaaat
      421 cggcggcgga tgattgttcg ttcactaaaa tatgcgtttt aatatagttt aataattcgt
      481 tcacggcctg ctgctgggcc agcggtaaat ccgttttttc cagcatttcc tgcagttgca
      541 acatgacctg aattaattcg gcgcgggttt ttaacgcaaa ttcgaagtag cggccaccgg
      601 cggttaatgc ggtacccagc gcccgcgcct gccccaccga ttccgcggcc gccaatagct
      661 cacgccaatt attgggagca ccgcgcacat ctttagttaa atagtgctcc gctgcaatat
      721 catcaataaa caccaaaata tttttaatca gccgattgtg ttgatcgatg gcatttaaca
      781 aacccatgct gtgcaggcgg cccgccaaac gtgcccaatg ggctgtgacc gccaaccaat
      841 ttacattgtc tttcacccaa tcacccacat cggcgatatg cagcagatcg cggctaatta
      901 actgctgcac ttcttccagt tgcggcagca atgagcgatc accactcata acaccagccg
      961 acaagccgcg atgtttttga atgtgcgcaa gtaaaatacg catagcctgc aaccagcgca
     1021 ccccctgcaa ttgttggcgg cagataattt ggcggcgcaa ataaaaacca aaggcaatca
     1081 agcctaaaac aattaaaggc aataattgaa tggcttggct tacagctccg aggggcatag
     1141 gcgtgggctc aagggattgc caatggcaac tgcaattccc gctccaaagg ctaggcagca
     1201 taagaggctg cgccttaaca ggtcgtacac ctaggatgct gtagcgagca gcggacaagg
     1261 cgcgcgccaa tcagaccgaa tagacgcgcc aaaataaagc caggaaaaag aatgcacgcc
     1321 cagcattggg gcgtgaaatt gagtgcaggc cagatactta acatcccctg gattaagtgt
     1381 ttagcctatc tattaaacgt gtgtttacag gctatgcaaa ccctacagct ggctaaaaaa
     1441 ctccatctac tgctgcgttt accatttttt acagaaccca gaatttacct taccaaagcc
     1501 tgcactccta gtgatgcaat cagagtgaat acacccagca ttaaacagta gcagagccgc
     1561 tacacaccta aacgcgcttg cataaaaccc tgcactgctt ttatatcatc gggcaatacc
     1621 gcgcagcgtt cttcgcggct aaataaatcc tgcatatagt ggggcaactg tggcaccaca
     1681 cccacaccgg atttttcaat cgcctcaggg aatttggccg ggtgtgcagt ggctaaacac
     1741 accatgggca cagataagtc gcggcgcgtt ttacgcgccg cctgcacacc aatggctgtg
     1801 tgggggtcca ataaatattc gctgctgtta aatacttcag ccactacgct gagcatttct
     1861 gcgtcatcca atttataact ggcaaataat tcctgcgctg aggccaatac ttcttgagat
     1921 aagctcatgc tgcccgcttt aaaatcatcc atcagctgcg aaattgcggc gccatctcgg
     1981 ttatacaatt caaacaacat gcgctcgaaa ttgctggaca ccataatatc catgctgggc
     2041 gataaagagt gaattaacgg ctttttgctg tggtcgttag cgctgatgca acgatgcaaa
     2101 atatcattgc tattggtagc tatgattaat tggtcgatgg gcagccccat acgcttagcc
     2161 aaatagcccg cgaaaatatc accaaaattg ccggtcggca cactaaagct gactttgcga
     2221 tccggcgcac ctaaggccag ggctgcgtaa aaataataaa caatctgcgc cataatacgc
     2281 gcccagttga tggaattaac cgccaccaac tgccgcccgg ccggtaaaaa actctgatcg
     2341 ctaaaactcg ccttcaccat attttggcaa tcatcaaaat taccctgcag cgcgatgtta
     2401 tgtacgttcg gcgcaagcac cgtggtcatc tgccgccgct gcacatcgga aacacggttg
     2461 tgtgggtgca aaataaaaat atccacattt tcacaataac ggcagccttc gattgccgcc
     2521 gaacccgtgt cgcccgaggt ggcgcccatc acaacaactt tttgttggcg cttggcgaga
     2581 ataaaatcca ataaattacc caaaaactgc agggcaaaat ccttaaatgc aagcgttggg
     2641 ccctggaaca actccagaat ccattcgttc tggccgatct gcaccaaagg cgcaatcgcc
     2701 ccatggcgaa attgggcgta ggacttggct ataattgctt ttaaattcgc ctcatcaatt
     2761 tcgccatcaa caaacggcgc aattatttta taagccagct cttgatagct cagactgcga
     2821 aaactcgcga tttccgtttg ggaaaattgc ggaattgatt caggcacata aaggccgccg
     2881 tcggacgcaa gcccagttaa tacaacatct tcaaacgata acttgggcgc tttgccacgg
     2941 gtactgatgt agttcacaaa ccaattctca ttaaattaaa gtaattacaa agattccacg
     3001 cgaatgcgca caaccgggcc gacaactgta tccaaggctt caattaagct gatcgcacgg
     3061 ttgaggtttt tttccaacac tttattggtc aataaaatta ccggcacttg tgtatcatcg
     3121 tctttgggct gtttttgaat cagcgcttcg atactaattc ccgcgtcgga taatatctgg
     3181 gtaatttttg ataatacccc cggctgatca gccgccgaca tacgcagata ataggaggtt
     3241 tcaatttctt ctatatccag gatagaaaaa ctattgagct ggtcatcttg gaaccccaaa
     3301 tagggcgcgc ggtattctgg cggcgccagc agcgtgcggc ttaattcgat aatgtcggaa
     3361 ataaccgccg aacctgttgg ctcagcgccc gcacctggcc cgcagtaaag cgttgaaccc
     3421 accgcatccc ctttaacgac tacagcattg gaaacgccgt caacgctggc aattaagcgt
     3481 ttttctggaa taaatgtcgg gtgcacccgc aattccacac ggttgtcggc atgtttgcgg
     3541 gcaattccca aatgtttgat gcgataaccc aattcatggg cgtaagagac atccaccggg
     3601 gcaattttac tgatgccttc tgtataaacg cgggtgaatt gcagcggcat gccaaacgcc
     3661 aaggagccta aaatagccag tttatgggcg gcatctatgc cttctacatc gtaggtgggg
     3721 tcggcttcgg cgtagcctaa ttgctgcgcc tcagccaaaa cactttcaaa agagcggccc
     3781 ttatctttca tctcggtgag aatgaaatta ccggtaccat taataatacc tgccaaccat
     3841 tcaatgcgat tggccgatag cccctcttta agaatacgaa taatcggaat accacctgcc
     3901 accgaagcct caaacgcaat actcacccct ttggcgcggg ccatggcaaa tagctcatta
     3961 ccatgttggg caatcagtgc cttgttggcg gttacgacgt gtttgccgtg ggcgatggcg
     4021 gtttcgatca gggttttagc agtggtggtg ccgccaatta actccacgag aatatcaacg
     4081 tcggggtttt tagcgacttc aaaaatatcc cgcgttaccg ccacatcgtg tgtatcacaa
     4141 ctgggattat ccttgcggca gcccacttgc actatgcgaa tttcacagcc agcgcgagcg
     4201 ttgatctctt gcccgttgcg ctggatgacg ttaaacgtgc cggaacctac tgttcctaaa
     4261 ccacaaatac caattctgac ggtcttcaac ctaaacctct gcaattttgg ggcggatcgg
     4321 gcaaaaagac cgccacctta caaagcccga ttacccctgt caacgagcag atcacagcga
     4381 ttactgacag cctttataag aacctaagcc agctcaaggc taattgcgga gcaacaaaaa
     4441 aggccccgca gggcctttta aaaattgctt tgcatcaagg cattagccct tgatattgag
     4501 catctgcgcc aaacgctcag gctccacaaa gcccggtacc atctggccat tttcaaacac
     4561 taacgcaggt gtgccggata cacccaattg ttggcccaat tgaaattgag cagcgattgg
     4621 gttgttgggg catacgttgt tggggatttc ttcgcggttt ttaagcttgg tcaatgctgc
     4681 ttttttatca gcggcgcacc aggcagatgc caatttatta aatgatgcgg tgttgagccc
     4741 tgcgcgcggg tagcccaggt aacgcacttc aatgcccagc tcattcatct tgccaacgtt
     4801 tttatgcagc atctgacagt agccgcaatc tacgtcggta aatacataaa tagatgcctt
     4861 gggcttgccc tctggcgaga agataatcat gtcatctttc ttgatctcag ccactgcctt
     4921 ggcgcgcagg ccattacgct cctcatcgga cacattaacc acttcagcgc cattaattcg
     4981 gtaaaaatct cccacgataa aactggtgcc atcggcgtta gtgtaaatag ccggcccctg
     5041 gcctaaagaa accttataca caccgggaat tgatgaacgc tgcacatctg atacggataa
     5101 atcgggtttg gcaccctgca aggttttatt gatgcgctgt ttaatctctt ccaagctgcc
     5161 ctcgttagct ggggctgcgg cgttttgccc ggttacaccg atgggcaatt ccacttgttt
     5221 gggtttgtcg gcggccattg cgccggcggc caaggccaag cagatagcgc cggtcaatag
     5281 agaggatgtc agcttcataa gggatctcaa ataataaatg tgattcggtt gcgagggcgc
     5341 aaagccgtat ggatcgccac agccttgcgg atggtggagg tgcgcatgag aatagcacca
     5401 gaccaagcaa atatgttttg gttcagttgg caacttgcgc tatcgcaaaa ccaccgcagc
     5461 cactagcctg cctcgcaccc gtttaaagag gtaaggtcac attcgcggca aacaaaaaag
     5521 gcccctaagg gcctttgtta actaccaagc tacttgctcg ttaatttttc tttgattcgc
     5581 gctgctttac cagccaggtc gcgcaagtaa tagagcttag cttgccgtac atcgccgcga
     5641 cgcttcactt cgatagaacc aatttgtggg ctgtgagttt ggaaggtacg ctcaacaccc
     5701 acgccgtggg aaattttgcg cacggtaaac gcagagttta agccgcgatt tttcttggca
     5761 attaccacgc cttcataggc ctgtaaacgc tcgcgctcac cttcaattac acgcacgtgt
     5821 acaacaacgg tatcgccagg tgcaaagctt ggcagatcgg ttttgagctg ctcgttttcc
     5881 agctcttgga tgattttgtt cttagaactc atagcatgct cccaacaatt tagcttcaca
     5941 gctaacaaat aaaaattcag tttaggtatc ggatttacgc ccagcctgat actgcgctag
     6001 cagcttcaac tgttcagggc tcaactccag tttttcaagc aaatccggcc tgcgctccca
     6061 ggttcgcccc agggactgtt gcaaacgcca attcgcaatt tttttatgat cgccagacac
     6121 caaaacctcc ggtacgcgat caccatcata ttcttccggc cgggtatagg cggggcaatc
     6181 caacaagccg cttacaaacg agtcctgcac cgctgactca gcggcaccta atacacccgg
     6241 tagtagacgc gccatcgcat ccaccatcac catggcggga agctccccac cactcaacac
     6301 ataatctccg atagaccact cttcatctac atagcgctgg acaaaacgct catccacacc
     6361 ctcgtagcgg ccggccacaa aaatcaacgc gggctcggtg ctgagccgct cagctgcaga
     6421 ctggtcaaac cgtttaccct gtgggcttaa ataaatcacc ttgctctgca aggctccact
     6481 cgccgccagc atgtctttcg cttgctggag cgctttgcgc agcggctcca ccatcatcac
     6541 cataccgggg ccgccaccaa aggggcgatc atccactgtt tggtggcggt caaaggcaaa
     6601 atcccgcggc gaaatacagt gcagcttaat caagccctgc tttaccgccc gcccggttac
     6661 gcctaaattc gcaaaggcgc taaacatctc gggaaacaag ctcagcactg caatgttcac
     6721 aagcaggctc ctgctatcta cttacctatt ttcaaattac ctatttttaa aactcgggat
     6781 cccaatccac tcggatgata ccgagtgcaa ggttcacctc aataacaaac tggcccggca
     6841 gataagggat caaccgctcg ccctggtcaa tcgaggtgcc atcgccgcgc actactaata
     6901 catcgtttga gccggtttcc agcaggctgt gcaccacacc caagtcctgc tcattaccgc
     6961 caaacaggct aaccacccgc aagccgatca actggctcca gtaatactca ccaacggcaa
     7021 gttcggctaa ctgatcgcgc tccacgtaaa tgctcgcctt acccagagca agcgcctggt
     7081 cgcgatcatc aatacctttg agcttaacca cggccccctg ggggcgctgg gcatagtcct
     7141 gaatagcgaa ctgcttaagc ccctgtggca gaccaagata ccagggcgcg taatgcaaca
     7201 aattgtcggc aggctcggtg aaggatttaa cctttagcca acccttaatc ccaaagaccc
     7261 cgacaatctg acctaccact aacttggaat tcatggccgc ggccgcagat cctcaagcac
     7321 cgcactccaa tgccgattaa gcagcggcaa cggcggcgtc tttagccaat tttgctacgc
     7381 ggtcagagca ctgggcgccg tttttcaccc agaaatccaa acgctctaaa tcaatgcgta
     7441 aacgctcttc tttaccgcgg gctaccgggt taaaaaagcc cagacgctcg ataaaacttc
     7501 cgcccagagc tttttcgctg tgggtcacag ttacgtggta gaaagggcgc ttttttgagc
     7561 cgccgcgcgc taaacgaatg cttaccatgg gtattttcct gtcggaatac aaaaatcaat
     7621 caaattataa gcgggccgcc caatatctta agggggaggc ctgcgaaagg cggcgaagtc
     7681 taatggaaag acttaagcct gtacagcccc aaggctatat ttgtgcggac taacgcccaa
     7741 ggcccggcgg tgtgccgccg cccatcatgc cgccaatgcc gcgcagcatt ttggacatgc
     7801 cgccaccttt gaattttttc atcatcttgg ccatctgctt gtgctgcttg agcaggcggt
     7861 tgatgtcttg gatatcggtg ccagacccca tggcgatgcg gcgtttgcgg gaattattaa
     7921 tcagctccgg gtcgcgccgt tcgccggggg tcatagagct gataatggcc tccatctgct
     7981 taaactgttt acccacatca gctttttcag ccgcagcggt gatatcgccc atgcccggca
     8041 gcttgccgag aatagcgccc ataccgccca ggttttgcat ctgctgcagc tggtcgcgca
     8101 ggtcttccag gtcaaaactt ttacccttct taaccttctg gatcagcttt tcagccttgg
     8161 ccttatccag cttttgctcc acatcttcaa tcagcgacaa cacatcaccc ataccgagaa
     8221 tgcgcgaggc caagcggtcg gggtagaaag gctcaagggc atccaccttt tcacccacac
     8281 cgaggaattt aatcggcttg ccggtaatgt ggcgcacgga aagcgccgcg ccgccacggg
     8341 cgtcgccatc ggctttagtg agcaccaccc ccgtcaatgg cagcgcatca ttaaaggcct
     8401 tggcggtatt caccgcatct tggccgatca tggcatccac cacgaacagt gtctcaatgg
     8461 ggttaatcgc cgcgtgcagc gccttaatct catccataag ggcttcatct atatgcaggc
     8521 gtccggcggt atccacaata accacgtcag caaagatctt gcggccatgg gctatggcat
     8581 tgcgggcgat atcgatgggg ttttggtcgc tgctggaggg gaaaaactcg gcggatactt
     8641 cagcggctag agtttctaac tgtttgatgg ccgccgggcg gtacacgtcg gcactcacca
     8701 cgaggacttt tttcttctct ttatctttta agaaccgcgc aagtttagct accgaagtgg
     8761 ttttacccgc cccttgcaaa cccgccatta acaccaccgc aggtggttgc atggccagat
     8821 tcagtttttc attggcctcc cccatcattt gggtaagctc ttgctcaacg attttcagaa
     8881 actgctggcc gggatttaag gcgcgcgaaa cttcttggcc gatcgcccgc ttgcgcactt
     8941 cattaatgaa gtccttcacc acggacaggg ccacgtcggc ttccaaaagt gccttgcgta
     9001 cctcccgcag ggtatcttgg atgttgtcgt gggtgaggcg ggctttgccg gttattttgc
     9061 gcagggatcg cgtcaaccgg tcggacaaac tctcaaacat agtagcttca ctaatagaag
     9121 aaaaaagaaa ggcggcgatt atacgggctt aggttaagaa aaccactctc gacgcacaaa
     9181 ccgccgactg ttgggcgcgc cagcaaacct tgccaaaggg gctctgggcg cactaccctt
     9241 tacggctttt cacccacccc tatccgcgcg gccacgtcat gatacccacc agtctttcga
     9301 ttctgtgcta cagcggcgcg tggatactgt taatgcgcag gttaacccag cgccaaccgc
     9361 tatcctcagg cctgattagc gggtttatct gtttggccct gagcctaaat gcctgggcta
     9421 ttttcgaaca gattcatgtc gccaccggct atcaattcgg cttttttaaa gttgcaccgc
     9481 tatttttttg ggcggccaat ttattgattt ttctcagcag ccttaagcgc ccattgcaca
     9541 gcttgctgct atttaccctg cccatgagcc tgaccgcaag cctggtcagc cttttagtct
     9601 ccaatagctc ggccactccc atcgccttgg ggccgggttt attaacccat gttttgctat
     9661 cgctgctggc ctacagcctg atgcttgccg ccctagtaca ggccctgctg ctggcctatc
     9721 agaaccagaa actgcgcagc aaacatccgg gcggctggct gcgcctgttg ccaccgctgc
     9781 aaaccatgga ggcgctgttg tttgagttgc tgtgggcggg ggagctggtg ttaaccctgg
     9841 cgattatttc cggcggtttg tttgttgaga atctgttagc tcaacacctg gcgcaaaaga
     9901 ctgtattt
//
LOCUS       DAOQGS010000085         9829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3218_length_9829_cov_4.800389, whole genome shotgun sequence.
ACCESSION   DAOQGS010000085 DAOQGS010000000
VERSION     DAOQGS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3218_length_9829_cov_4.800389"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1916)
                     /locus_tag="PKZ52_04840"
     CDS             complement(75..1916)
                     /locus_tag="PKZ52_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNG59130.1"
                     /translation="MPHAHAEFDYIVVGSGAGGGPLAANLAKAGKKVLLLEAGGDDEP
                     YNYQVPVFHPNASEDESLRWDFFVRHYADDAQQQRDSKMTAEKNGVLYPRSGTLGGCT
                     AHNAMITVYPHNSDWDYIASITGDQSWRSDNMRNYFQRFENNRAHPFKRLWAMLGINP
                     ARFGFNGWLHSSMPNPGTDILKDKQLLELLKDSAVSALKAIGRPVESIGRGLKGLLDP
                     NDWRLVKQSAEGLRYTPLATKNGKRMGTRDYIRAVAKEYPDHLVVRTHALVSRVLLDE
                     NNTAIGVEYQIGKHLYGADPRANGQAPEGYEQEFAKREVILSGGAFNTPQLLMLSGIG
                     PEEELARHKIPLRVNLPGVGRNLQDRYEVGIVSEMNQPFSLLEGSTFRAPEQGETPDP
                     QFKDWLEGKGVYTTNGAVLAAILRSSPERAEPDLFVFALAGNFYGYFPGYSKIITEGH
                     KYFTWAILKAHTNNTGGVVKLRSNNPRDVPDINFHYFDEGTDTAGEDLESVINGVEFV
                     RKMTAGASDVIKQEIIPGHEVSSREAIGQWIKDNAWGHHASCTCKIGADDDPMAVLDS
                     QFRVRGVKNLRVVDASVFPKIPGFFIVSCVYMISEKAADVILANEKA"
     gene            complement(2600..4750)
                     /locus_tag="PKZ52_04845"
     CDS             complement(2600..4750)
                     /locus_tag="PKZ52_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59131.1"
                     /translation="MTKKTSLILAGLGVIGLIAWWASQKPDPVDTPKAEGKTPADFPQ
                     TASSWYDAMDDGVSLTDEERKGRNTWLIWTGGNQEFWNHMATHSFGTVDLLKILDSRK
                     RSERFDWYGVINDPDMQQANKPDAYGLWLDTPKGDLPKDVDPKVYGLPSGIVGLRLYP
                     NPAFDEKAKAAWNAERYYSDQDYYYNPKLVRPYMVGMSCGFCHVSFHPNHPPENPAEP
                     KWANLSNNIGAQYMWISRVFGYDLKPDNFIWQIFNTMQPGAIDTSFIATDNINNPRTM
                     NAIYNVGARLGVGQVEKQGPQNMHFEGQKSEMPVPHILKDGADSVGILGALSRVYVNI
                     GQFHQQWFENHNPILGGKPQTPFPVEKAQKNSVYWQSTAERVGPLAAFFLKAAGPMPL
                     AATAEGKALLTEDEDTLKQGKLVFADNCASCHSSKQPKAAKGSDEYKTQMRELVLEDD
                     FLENNYLSTDERIPVTELKTNACSALATNAIKGHVWDNFSSDTYKNLPAVGPIEVQHP
                     LTGATFKYTPPGGGPGYTRVASLVATWASAPFLHNNSVGTYNQDPSVQGRVKAFDDAI
                     DKLLNPEKRLGLASVYRTTEESWLRVSPSFIPKFLQPLLGELKDDDGNIAIGPIPKGT
                     PVNLLVNIDMTLDDVEKIKNNLKVLLKVKKTLKEIHKNNLTGDAAAEALKPLVDDLIS
                     ISKCPDYVTDRGHYYGSDLSAADKHALIEYIKYF"
     gene            complement(5184..5630)
                     /locus_tag="PKZ52_04850"
     CDS             complement(5184..5630)
                     /locus_tag="PKZ52_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNG59132.1"
                     /translation="MQWTPHVTVATVVEQNGKFLLVHEQSDGRAVYNQPAGHWDEHET
                     LFEAARRETLEETGWVVELSYLLGISHYISPINGFTYLRITFVATPLEQIAEAKLDAD
                     IIEAVWLSLDEIQTRQTHLRSPLVLNDINRYLRGEQFPLSLIQHIS"
     gene            complement(5636..6253)
                     /locus_tag="PKZ52_04855"
     CDS             complement(5636..6253)
                     /locus_tag="PKZ52_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNG59133.1"
                     /translation="MATLILFNKPFAVLSQFSQDGDKTTLAQFIPVPGVYPAGRLDFD
                     SEGLLLLTDNGQLQHQIAHPSQKWCKTYWVQVEGIICAAALEQLRQGVVLNDGKTLPA
                     EAWAMPEPAIWPRNPPIRERKSIPTSWLCLRIKEGRNRQVRRMTAAVGYPTLRLIRTQ
                     IGDYELGALTPGAYTELHLDDGAAVKPTRRRPQKARTPSIHQHRK"
     gene            6356..7582
                     /locus_tag="PKZ52_04860"
     CDS             6356..7582
                     /locus_tag="PKZ52_04860"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNG59134.1"
                     /translation="MNKIKVANPVVELDGDEMTRIIWSFIKNKLILPYLDLDIKYYDL
                     GMESRDATDDQITIDAAEAIKKYNVGIKCATITPDEARVEEFKLKQMWKSPNGTIRNI
                     LDGTVFREPIICSNVPRLVPGWTQPICIGRHAFGDQYRATDFVTKGKGKLTITFQGED
                     GNTISHEVYNFKGDGIALAMYNTDESIRGFARSCFNMALTKKWPLYLSTKNTILKKYD
                     GRFKDIFAEVYEQEFLYSFKAAGITYEHRLIDDMVAACLKWNGAFVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLVTPDGKTMEAEAAHGTVTRHYRQHQQGKPTSTNPIASIFAW
                     TRGLAHRAKLDDNAELTRFCRALEEVCVETVESGKMTKDLAVCIYGDAVTKDQYLTTE
                     GFLDALDANLKAKLSA"
     gene            8713..9090
                     /gene="clpS"
                     /locus_tag="PKZ52_04865"
     CDS             8713..9090
                     /gene="clpS"
                     /locus_tag="PKZ52_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNG59135.1"
                     /translation="MGNLEILQLPLVGSHRSNLPLDDDAGLAVESQKPKLKRPPLYKV
                     IMLNDDYTPMEFVVEVLEVFFGMDREKATSVMLAVHTQGKAVCGIYSRDVAETKAAQV
                     NQCARDHEHPLLCEIEAVEDEDP"
     gene            9115..>9829
                     /locus_tag="PKZ52_04870"
     CDS             9115..>9829
                     /locus_tag="PKZ52_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNG59136.1"
                     /translation="MLSKELEVTLNTAFKGARSKRHEFMTVEHLLLALVDNESAANVL
                     RACGANLSALRQDLIEFVDSTTPLIPESDSERETQPTLGFQRVLQRAVFHVQSSGKSE
                     VTGANVLVAIFSEQESQAVYYLKQQSIARIDIVNFITHGITKVSGSSGNSHPENNHQQ
                     EHHEDESSVGPDSLGTNPLDSFATNLNELASQGRIDPLVGRDYEVERVVQILSRRRKN
                     NPLLVGESGVGKTAIAEGLA"
BASE COUNT         2454 a         2258 c         2444 g         2673 t
ORIGIN      
        1 gcagacagga gtccctcagc acaaaccgcg cggatgaaat gtggccgctc catggctggt
       61 tcggctgcgg atatttatgc tttctcgttg gccaaaatga catccgcagc tttttcactg
      121 atcatgtaaa cacatgaaac gataaaaaag ccgggtattt ttgggaatac agaagcatcc
      181 acgacacgta gattttttac accgcgcacc ctaaattgac tgtctaaaac cgccattggg
      241 tcgtcgtcag cgccaatttt acaggtgcaa gaggcgtggt ggccccaggc attgtcttta
      301 atccattggc cgatggcctc gcggctggag acctcatgtc ctggaatgat ttcttgttta
      361 atcacatcgc tggcgcctgc agtcattttg cgcacaaatt ccacgccatt gataactgac
      421 tccaaatcct ctcctgcggt atcagtgcct tcatcaaaat aatgaaaatt aatatccggt
      481 acatcccgtg gattattgct gcgcaatttc actaccccac cggtgttgtt ggtgtgagct
      541 tttaaaatgg cccaggtaaa atatttatgg ccttcggtaa tgattttaga ataaccgggg
      601 aaatacccgt agaaattacc tgccagggca aatacaaata aatccggttc ggcgcgctct
      661 gggcttgagc gtaatatggc agctaatacg gcgccatttg tggtgtaaac ccctttgcct
      721 tccaaccaat ctttaaactg tgggtcgggt gtttcgccct gctcgggggc gcgaaaggtg
      781 gagccctcta gcagggaaaa gggttgattc atttcgctga caatacccac ttcgtaacga
      841 tcttgtaggt tgcgcccgac gccgggtaaa tttacccgca ggggtatttt atggcgggct
      901 aattcttcct ctgggcctat gccggataac attaacaatt gcggagtatt aaatgcaccg
      961 ccggaaagaa tcacttcgcg cttagcaaat tcctgttcat atccttccgg tgcttgcccg
     1021 ttcgcacgcg ggtcagcgcc atataaatgt ttgccaattt gatattcgac gccgatcgcc
     1081 gtgttatttt catccaataa cacacggctt accaatgcgt gagtgcgcac cactaaatga
     1141 tcgggatatt cttttgcgac cgcgcgaatg tagtcgcggg tgcccatgcg cttaccattt
     1201 ttggttgcca ggggtgtata acgcaggcct tcagcacttt gtttcaccaa acgccagtca
     1261 ttgggatcta gcaggccttt taaaccgcgg ccgatagatt caactgggcg cccaatggct
     1321 ttaagtgctg acacggcaga atcttttagc aattcaagca actgtttatc ttttaaaata
     1381 tcggtgcccg gattgggcat actggaatgt aaccaaccat taaaaccgaa acgagcagga
     1441 ttgatcccca gcatggccca caggcgttta aatgggtggg cgcgattatt ttcaaagcgc
     1501 tgaaaataat tgcgcatatt atcgctacgc caggattgat caccagtgat cgaggcaatg
     1561 tagtcccaat cgctattgtg agggtatacg gtaatcatgg cgttgtgggc cgtacatcca
     1621 cctaaggtgc ccgagcgcgg gtacagcacg ccgttttttt ctgccgtcat tttgctgtcg
     1681 cgctgctgtt gtgcgtcgtc tgcgtaatgg cgtacaaaaa aatcccacct taggctttca
     1741 tcttctgaag cgttagggtg aaaaaccggc acctgataat tatagggttc atcgtcaccg
     1801 ccagcctcca gtagcagtac ttttttacct gccttggcta ggttcgctgc aagggggccg
     1861 ccgcctgcac ctgagcctac aacaatgtaa tcaaattctg cgtgggcatg gggcatatag
     1921 attctccgaa aattaagctg tgtttgcggt atatataggg ataaatacaa ccagatgttg
     1981 attagatacg gctgttttta atttaagggc acctctgatc aaaaggggtt ttcagaaata
     2041 caccagcaag ttacttcgaa accgtcgcag gctctggcct gcgacggttt cgaaaacacc
     2101 cattaatcag agattccttt atcagctggc ataaagcttg tgggccaatg ctaaaaaata
     2161 cagctcggtg tacaaaatat cttatcttta gaacctggtt ataggttctc tagtaataat
     2221 tcgggggcta aatatggatt caaccattcc cagattttat tccgtggcaa taatccttat
     2281 gcggccccac ttaatcagcc tgcgaggcca aactcttttt taggcatttt tatcaaaatg
     2341 gcccttgagt gtgttgagaa tgtccagcaa tagggcgggg cttggcgaaa tatggcagcc
     2401 caaacctaac agtttgctaa atattacctt gcgagcgtgg gcggcgttaa atttagcaaa
     2461 aaatcccaat tactgtgtgt aaactccagt cttttcgcca aatttgcctt gccagagcct
     2521 cgccgcctag gtatttcaac aagctgcaaa gatggttaat agccgttaga gggaaacagc
     2581 acatccttgt gtgccagtgt taaaagtatt tgatgtattc aatcagcgcg tgtttatccg
     2641 ctgcggatag atccgagcca tagtagtggc cgcgatctgt cacatagtca gggcatttgc
     2701 taattgagat taaatcatcc accaaaggct ttaaggcttc ggccgcagca tcgccggtta
     2761 ggttgttttt gtgaatttct tttaaggtct ttttaacttt taataacact tttagattgt
     2821 ttttaatttt ctcaacatca tccagtgtca tatctatatt caccaaaagg ttaactggcg
     2881 tgcctttggg aatggggcct atagctatat tgccgtcgtc atcttttaat tcacccaata
     2941 aaggctgtaa gaacttcgga ataaaactag gggagacacg cagccagctt tcttctgtag
     3001 tacggtaaac cgaggctaaa ccgagacgtt tttcggggtt gagtagctta tcaatggcgt
     3061 catcaaaggc ttttacgcgg ccttgcacgg atggatcttg gttataggtg cccacggaat
     3121 tattgtgtaa aaacggcgcg cttgcccaag tggccactag gcttgccacc cgtgtgtaac
     3181 ctggcccacc gccgggcggt gtgtatttaa aggtggcacc ggtaagcgga tgctggactt
     3241 ctatggggcc tacagccggt aggtttttat aggtatccga ggaaaaatta tcccacacat
     3301 ggcctttaat agcgttagta gcaagtgcac tacaggcatt ggtttttaat tcggtgacgg
     3361 gaatacgctc gtcagtagac aaataattat tctctaaaaa atcgtcctct aaaaccaatt
     3421 cacgcatttg ggttttgtat tcatcggagc ctttggcggc tttgggttgt ttgcttgagt
     3481 ggcagctggc gcagttgtcg gcaaaaacta atttgccctg ttttaatgtg tcttcgtctt
     3541 cggttaataa ggctttaccc tcagccgttg ctgccagcgg catggggcct gccgctttta
     3601 agaaaaacgc cgccagtggg cctacgcgtt ctgctgtgga ttgccaataa acggaatttt
     3661 tttgcgcttt ttcaaccggg aaaggggtct gtggtttgcc acctaatatg gggttgtgat
     3721 tttcaaacca ctgttgatgg aattggccga tattgacata aacacggctg agcgctccga
     3781 gaataccgac cgagtcggcg ccatctttta agatatgggg caccggcatc tcactttttt
     3841 gcccttcaaa atgcatattt tgcgggcctt gtttttctac ttgccctacg cccaagcggg
     3901 cgcccacgtt ataaatagca ttcatggtgc gtgggttgtt aatgttatcg gtagcaataa
     3961 atgaagtatc aatggcgccg ggttgcatgg tattaaaaat ttgccagatg aaattatcgg
     4021 gttttaaatc gtagccaaag acgcggctaa tccacatgta ctgcgcgccg atattattgg
     4081 agagattggc ccatttaggc tcggctggat tttccggtgg atgattgggg tggaatgaaa
     4141 cgtggcaaaa tccgcagctc atgcccacca tataggggcg gactaatttg gggttgtagt
     4201 aataatcttg atcgctgtag tagcgctcgg cattccaggc cgctttggct ttttcgtcaa
     4261 acgcagggtt gggatagagg cgcaggccaa cgatgccgga gggcaagccg tagactttgg
     4321 ggtctacatc tttgggaaga tcgcccttgg gagtatccaa ccaaagcccg taggcatcgg
     4381 gcttattggc ctgttgcatg tcagggtcgt tgatcactcc gtaccagtca aagcgctcgg
     4441 aacgcttgcg cgaatcgagt attttcaaca aatcaaccgt gccaaaactg tgggtggcca
     4501 tgtggttcca gaattcttgg ttgccaccgg tccaaattag ccaagtattg cgccccttgc
     4561 gctcttcatc agtgagggag acgccgtcat ccatggcgtc ataccaactg gatgcggttt
     4621 gtgggaaatc agctggggtt ttgccttcag ctttaggggt atctacagga tcgggttttt
     4681 ggctggccca ccaggcaatt aggccgataa cacctaagcc tgcgagtata aggctggttt
     4741 tttttgtcat tttaaactcc aactcttgcg cgcgatcggg tgcaatccag atctggcgta
     4801 tttattgatc aatagagggg tggcagataa acagcggcat actgcagggc agcaaggcgg
     4861 ctatcatata gtgcttaact aattaattca aaactctaac ccgggcagat tgttattaat
     4921 cgcgggattt aatagagcgt taggaagttt aagtgcagat gtgtaaaaag tgctgtgaac
     4981 tattaacctg cgtatcggcg gctaaattta accggctacg tcaattgatt aattcgatag
     5041 gcagtattgc tggtgtgcat gcaatgcaag tacttactgc agcggtattt tagtgatgtt
     5101 gtgttaattg gtgttatggc agccatcggg gcgccgggcc tgtgatagtc gcccactatc
     5161 cccgctacat gatcagaata tccttaacta atgtgttgga tcagcgacag aggaaactgc
     5221 tcgccgcgca aataacgatt gatatcattc aatactaaag ggctgcgcag atgggtttgg
     5281 cgggtttgaa tttcatccaa gctcaaccaa actgcctcaa taatgtccgc atctaattta
     5341 gcctctgcaa tttgctcaag cggcgtggca acaaaggtaa tgcgtagata ggtaaagcca
     5401 ttgatggggg aaatgtaatg gctaattccc aataaataag aaagttcgac cacccaaccg
     5461 gtttcttcta gtgtttctcg gcgcgcagct tcgaataagg tttcatgttc atcccagtgg
     5521 ccagcgggtt ggttatacac agcccgacca tcggattgtt cgtgtactaa caaaaattta
     5581 ccgttttgtt cgaccacggt ggcgactgtg acatggggag tccattgcat agcgattatt
     5641 tccggtgttg gtgaatagag ggggtacgcg ctttttgagg gcgacggcgt gtaggtttga
     5701 cggccgctcc atcgtctagg tgtagttccg tataagcacc aggcgtgagg gcgcccaatt
     5761 cgtagtcgcc aatttgggtg cgaattaagc gcagagtcgg gtagccgact gcagcggtca
     5821 tacggcgcac ctggcgatta cgtccttctt taatacgcaa gcatagccag gaggtcggta
     5881 tggatttgcg ctcgcgaatg ggcgggttgc gtggccagat ggcgggctct ggcatggccc
     5941 atgcttccgc gggcagtgtt ttaccgtcgt ttaacacgac cccttggcgc aactgctcca
     6001 aggcagcggc gcagatgatg ccctccactt gaacccaata ggtcttgcac cacttttggg
     6061 agggatgggc aatctggtgt tgtaattgcc cgttatcggt caataataaa agcccttcag
     6121 aatcaaaatc caaacgcccg gccgggtaca cgccgggcac ggggataaat tgcgccaagg
     6181 ttgttttatc accatcttgg ctaaattgtg agagcacagc gaagggcttg ttaaatagaa
     6241 ttaatgtagc catggggtca ggggttattt cagctcaggg ctcaatgcta tactcgccgg
     6301 gtttttttac cgttccacaa gcacaggctg ttagccgtta aaaataggaa gactcatgaa
     6361 taaaatcaaa gtagccaatc cggtagtcga gttagatggc gatgagatga cacgcatcat
     6421 ctggagcttc atcaaaaaca aactgattct gccctacctc gatttagaca tcaaatatta
     6481 cgacctgggc atggaaagtc gcgacgccac tgacgaccaa attaccattg atgccgctga
     6541 agccatcaag aagtacaacg taggtattaa gtgcgccacc atcactcccg acgaagcgcg
     6601 ggttgaagaa tttaagctca aacaaatgtg gaaatcccct aacggcacca tccgcaatat
     6661 tctcgatggt actgtatttc gcgagccgat tatttgtagc aacgtacccc gtttagtacc
     6721 cggctggaca caacctattt gtattggccg ccacgccttt ggcgatcagt accgcgcgac
     6781 cgattttgtc accaagggca agggtaaatt aacaattacc ttccaaggcg aagacggcaa
     6841 caccatttcc catgaagtct ataacttcaa aggtgacggc atcgcgcttg cgatgtacaa
     6901 caccgacgaa tcgattcgcg gttttgcgcg ctcatgtttt aacatggctc ttaccaaaaa
     6961 atggccttta tacctctcca ctaaaaacac cattttgaag aaatacgatg gccgttttaa
     7021 agatattttt gccgaagtat acgagcaaga attcctctat tctttcaaag ccgccggcat
     7081 cacttatgag caccgtttaa ttgacgacat ggtggccgct tgtttgaaat ggaacggcgc
     7141 atttgtgtgg gcctgtaaaa attacgacgg cgacgtgcag tcggatacag ttgcccaggg
     7201 ctttggctcc ttaggtttga tgacctctgt gctggtaacc cccgatggca aaaccatgga
     7261 agctgaagcg gcccacggca ccgtaacccg ccactaccgc cagcaccagc agggcaaacc
     7321 tacctccacc aacccgattg catcaatctt tgcctggacc cgtggccttg ctcaccgtgc
     7381 caaactagac gataacgccg aacttacccg tttttgccgc gcgctggaag aagtctgcgt
     7441 ggagactgta gagtccggca aaatgaccaa agacttggcc gtatgtattt atggcgatgc
     7501 ggttaccaaa gatcagtacc tcaccactga aggcttttta gatgccttgg atgcaaattt
     7561 aaaagccaaa ttgtcggctt gataggttag cggtaaacaa aggccagctc gaatcttatc
     7621 gagctggcct tttttattgt gcttcgcatt taaaataaat cctctcagca aagaaattct
     7681 tttttcaaag cctctaaaac gggattttgt attttgttgt ggaaggagct tgtagtgggg
     7741 ttttagggat gacgtggcgt atttctgaac atactaatag gccagcggtt ccccaaatcg
     7801 ccctgcctat atgcgttgga aattcgcttg aagcggcgat cccgcgatta aaaaggggct
     7861 ctcagcgcgt cgtagcgaga tggcgtgtga tttttagcct gaagccgatt gagcgtgaca
     7921 tcaaaagagc gcagaaaata ctgtttaaat gtgtacttct cgcgcagatt ttaaccccct
     7981 taattgtttg tgtgctctag gttttctttg gtgcgccccc atctacgcca aggcccgtgc
     8041 aggctgctcc tttgaattca gatgtaatca gagccagctg agcctatggg caagcctagg
     8101 tggctgcaaa aaagttacta aaatcggcac attctccctt ttttggtcta agagtggatt
     8161 tggatataaa gcgttgttgc caagaggtgt accttggcaa gcgtgttttg ggcgctagag
     8221 tttccgccgg tgtattgagt atgagtgcgt ctgggcattc atctgattaa gtctaaattt
     8281 gggaatatgt cgaaagcgca caagttcctt cccatagttc ggttgcggcc tgcctgttcg
     8341 aatatcctaa tgcaaaggtt tcctccacca agcaatctaa tctgcactgg taaggcctta
     8401 ttagaggctg cctcgtataa atggcagcac ggcacttgtt tttatgcccc aggccccaat
     8461 tgttttgctt tgaaaacgct ctttttattc aaattttttt gcaacataaa gctgggcggg
     8521 tgttctatgt atagcgacct ggtcttaaac atgagaaatc cctttcaaat ggtgcataaa
     8581 gtgatcgttt tatcatcaaa taggccttga agtgacacag ttctgactgt tttatgcaca
     8641 tttttgcgga tgttttgctg ctagtgcgat agaatcagaa ctcgtttgct tagctgatat
     8701 acacgggttc tcatgggtaa tctcgaaatt cttcaactac cattagttgg aagccatcgt
     8761 tcaaacctac cgttggatga cgatgctggc ctagcagtgg aaagtcaaaa gcccaagctc
     8821 aaaaggccac cgctgtataa ggtaattatg cttaacgacg attacacacc aatggaattt
     8881 gtcgttgagg tgctggaagt gttttttggt atggatcgtg aaaaggctac tagcgtcatg
     8941 ctggcggtac acacacaagg aaaggctgtc tgcggcatct attctcgaga tgttgccgag
     9001 actaaggcgg ctcaggtcaa tcagtgtgct cgagatcatg aacatcctct gctgtgtgaa
     9061 attgaagcag tggaggatga ggatccctag atttataaaa ggttaggtgt tgcgatgttg
     9121 agtaaagaac ttgaagttac cttaaatact gcgtttaaag gtgcacgttc caagcgtcat
     9181 gaattcatga cggttgagca cttgttattg gcgttagttg acaatgaatc cgcagcgaat
     9241 gttctgcggg cttgtggggc taacctcagc gcactccgtc aggatctgat cgaatttgtc
     9301 gattccacga cacctttgat acctgagtct gatagcgagc gcgaaactca acctacctta
     9361 ggttttcagc gtgtgctgca gcgcgccgta ttccatgtgc aatcctccgg caagagcgaa
     9421 gtaaccggcg ccaatgtgtt agtggccatt ttcagcgagc aggagagcca agctgtttat
     9481 tacttgaagc aacaaagtat tgcgcgcatt gatatagtca attttattac ccacggcatt
     9541 actaaagtat ctggttcatc gggtaattct caccctgaga ataatcatca gcaagaacac
     9601 cacgaagatg aatcgtctgt tggcccagat tctttgggca caaatccgtt ggatagtttt
     9661 gccactaatt taaacgaatt ggcttcccaa ggccgcattg accccctagt gggccgcgat
     9721 tacgaagttg agcgcgtggt gcaaatacta tcccgtcgcc gcaaaaacaa tccactcttg
     9781 gtgggagaaa gcggtgtagg taaaaccgcc atagccgaag gcttagcaa
//
LOCUS       DAOQGS010000086         9826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3221_length_9826_cov_5.220244, whole genome shotgun sequence.
ACCESSION   DAOQGS010000086 DAOQGS010000000
VERSION     DAOQGS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_9826_cov_5.220244"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1727
                     /locus_tag="PKZ52_04875"
     CDS             249..1727
                     /locus_tag="PKZ52_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG59137.1"
                     /translation="MALKLAHKLLLTHVLIIAVLATVFLTFSYLSNQSLVSDAMNGID
                     ETVMEELAPMLSQHYQTEGGFNALLKDPDHWRGLVDGTFFKVFFSLSPAPSSKPISSA
                     SGDKNQPQTPSFAPASNLPACEPPFPTFLQRLALLDKDQQVLIAAPKAAAEYYQTPIK
                     LQGEIIGWLRVGKINVDSLPYAHYFFKHQLRMALWAIACAAALALLLGYFLSRQITGP
                     LMTIASETQKLASRDFSVNIQVHTGDELQALAQHINVIAGEIKYFNQRQKQWLMDISH
                     ELRTPITILYGEVTAILDNVTKADSNTIKSLQEEVVHLKRLVDDLHALSVLDDGEFHL
                     SCEPIELGELIQQQASRYAERFETLGLELHIHCAGEVVWVNADRDRLGQVLRNLLENC
                     LRYTHAPGVVSLNLHVHASAVHLILEDSGPGVPAEVLAKLFDRLYRVDAARTRAGGGA
                     GLGLAICKTIILAHAGSIEAFLTEQGGLGVRIILPRTRVEAV"
     gene            1724..2398
                     /locus_tag="PKZ52_04880"
     CDS             1724..2398
                     /locus_tag="PKZ52_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG59138.1"
                     /translation="MSQIILVVEDEIKIAELIGKYLTLEGYEPILVDNGVDALAIFKQ
                     RQPALVILDVMLPGMNGLSVCERLRAVSDVPIILLTARVQDVDRLIGFASGADDYVCK
                     PFNPQELMARVRAILRRIKPAAAPTTLSFDKITIDQAQHGVWVSGVKVQLTQIEFKLL
                     LQMLEQPAKVFSREELLLSSRGKYTESYERTIDFHVKNLRKKINIDAGSNYIHTVYGL
                     GYKLIG"
     gene            2681..3967
                     /locus_tag="PKZ52_04885"
     CDS             2681..3967
                     /locus_tag="PKZ52_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG59139.1"
                     /translation="MLLFPAQRGIGLANTACTPRTLRGKVPGVQDEVFMRTGLINSIT
                     EVEASLWQQLWATDYPFIQHDFMALLEDSKCVGGRSGWTPRHILIWQEDRLVAAMPGF
                     IKTHSYGEYVFDWSWADAYGRHGLEYYPKWINAMPFTPATGPRLAIAPGADGAAVMQA
                     LNEAWQELLKQGAHSLHSLFPDNASLPRLPAAWHTRWGCQYHWFNNGFRDWQDFLAQC
                     NSRKRKMMVKERQKVQALNLTIAVKSGTELTGEDWQAFYGLYQNTYAKRSGRPGYLTQ
                     DFFTGLQAILPGQIHRVDVSRSGQLIAAALYFSDGETLYGRYWGCSEEHDGLHFEACY
                     YQGIELAIKLGLKRFDPGAQGEHKIARGFTPVLTCSKHLLAHAGFHEAVGEFCREEQM
                     GIERYIQSARQALPFKEGYVCVEPGILQKNKSEIIY"
     gene            4155..4394
                     /locus_tag="PKZ52_04890"
     CDS             4155..4394
                     /locus_tag="PKZ52_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59140.1"
                     /translation="MQSLLGRWLGLLCLALAAYCFMQVGQGLMAGEITKLSKYSQEII
                     QRAAQPERFWIAIGFWTIGATILLVTGIKKITRRD"
     gene            4913..6610
                     /locus_tag="PKZ52_04895"
     CDS             4913..6610
                     /locus_tag="PKZ52_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNG59141.1"
                     /translation="MSNDDHSKFRLVLFTPYHEGFFYGEIINHVRLLCKLKNYALDVI
                     ATHSFGEYHSNFWLANVGAVIIVRNAISPNFAAQLSARGIPCVSIAYDYFPAPIHMVC
                     SDNRQGVKLAFEHLIAQGHKRIAFIGDLTQYDLRKRYESYCEEHSRHNLLLDEELSFF
                     TQNTTYLGGFNAATEFVKRDCDATAVIAAAGLLAEGFKSRLNDISPMLYESLEIVAFD
                     VMPVQILSNKSTMAIDLNLMFIAHNCITIIERIRAGHDVEHQTKIDCRLVTLPAFGVA
                     APDDLAPCLEDPSFTNSPYVKSLITNMYEWSMAIAGTGLSDVMSLSPLFDQLLKMGLL
                     FRLVKGAGAVESARLIKVCKRRETLLMDKYDPANQCLSADVQNHFADHFEHAEYELTT
                     SIPIVINGKLWGIFTACGSNEIGDSRNNYLALLCYLDLSIQFLTKNIISSLDAAADKN
                     PATAAPVAEHGGVVTWDFAKGVVEWSDEALAFLGLTSPLEKNIYRYMELDDRVHEDDQ
                     SALRAGLYKLHEQAVPLHINVRMKSKSGGYLLIRIDGQADAARQLMLFELRQELGAL"
     gene            6639..8534
                     /locus_tag="PKZ52_04900"
     CDS             6639..8534
                     /locus_tag="PKZ52_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59142.1"
                     /translation="MSYPWVWAILFLATLGVLLICLAKLRQQQKDLAEAKHANSISSE
                     EQDKNIVLAFLVDQFDRTKRLNDNVYFRHDELDRHACSLRSAYLKIEEKSLKNNADHR
                     RYHLAINKSLVKLLKIIRDDAIKTIDVEGVSDRSLHEALDGSKLSAQEKSELLNQIEE
                     FCEQLKSGAIGVEFRKKRYKQLLAMIKNVENTEERVAMKKLLLLAESADASFEHYSNL
                     SESIFNAGSDVAALQGVLDGSGPGLSSQVQAQLDALGKENMKLYSQVQMLRQWSKENK
                     KLAYPEGVSLSSATINDVSEEIINKHEEELKALKATVRNQKLTIFELENSLDEYRKSE
                     HAKGAKSDYEQEFGALTRRLTATEDLISEQEKNVGRMRTGLEALNGTEAIGEERETLM
                     QCIEKLKKEVTEAKEAYTSKNVEFDFISELIAVGSLEDLALLVFQYFCDQKCDPFLLI
                     SHKGRNIEMSATGALSVKDKTIINAMMPNDASKDDERFQLQFRYHNFGGILRFLPGST
                     ENYDSCEALLKMAKITDAFIEKISSGQNMRGLKKQLEQCSNDVKQLAQTVDIGFEQIC
                     QAHRQLISNHITRGQNLLRVKSATAAQIACLKQMETDMLEDMAPNGSVRIKMRKEFLA
                     LVKKIEQ"
     gene            complement(8776..>9826)
                     /locus_tag="PKZ52_04905"
     CDS             complement(8776..>9826)
                     /locus_tag="PKZ52_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59143.1"
                     /translation="IGAVYTALGDENGMPSDGTSHAYQELARALSGYNGSLDEALTDA
                     NLLKSVAKNAEQAVQKRYQDKELPLPSQSGDEAKFDVIVERLQALAPLINQLLPPVKN
                     TTAEDARAGARAVEVLGVKAVNEKEKDPATDRAFKFFRDNAAANQELLAGLGSEQADI
                     SQLTEHMFSDEDVKNPQGIKHLGQVDASAVPFNSVAGKQLHVADMDLGQKPNNLKDIE
                     FELYFAGDGAASAGSFTACAKYIKKAKADGSLGKGNSRGELIKGFWSQMDNQNKTHNY
                     SLLLTFEFLGAKYQAIMKSVGSRTIDGTLYQALRFDNNDEYRIWYSAKGLEAQTTLPT
                     SSSECQSRLPSRVGI"
BASE COUNT         2494 a         2175 c         2523 g         2634 t
ORIGIN      
        1 aaatacagat gtttaaacta gagccacacc agcattgcag taatttaatg cgatatatct
       61 gaggagttta gccttcgagt ggattctaaa gtctgattgt gagttttttg tgaggaactg
      121 gtgtggacaa aatgggcctg ccaccagctg tgattgagta gagatttggt ggcccgcttg
      181 cttccactat ggggctttgc catattgatt acactatagg aaatcaaaaa caattttggc
      241 ggtaccccat ggcactaaag cttgcacaca aattattgct gacccatgta ttgattattg
      301 cggtactggc aacggtcttt ttaaccttta gttatttgtc aaaccaatct ttggtgagtg
      361 acgccatgaa tggcattgac gaaaccgtta tggaggaatt ggcgcccatg ttaagccagc
      421 actatcagac tgagggtgga tttaatgcct tgttaaaaga tcctgatcat tggcgcggtt
      481 tagtggacgg aacttttttt aaggtatttt tttcactttc gccagcaccg tccagcaaac
      541 caattagctc agcttcgggc gataaaaacc agccgcaaac gccgagtttt gcaccggcat
      601 ctaacctgcc agcttgtgag cctccctttc ccactttttt gcagcgcctg gcgctgttgg
      661 ataaagacca gcaggttctg attgcagcgc ctaaagcagc cgcagagtat tatcaaacac
      721 caattaaact ccaaggtgaa ataatcggct ggctgcgggt gggtaaaata aatgtcgatt
      781 cattgcccta tgcgcattat tttttcaaac accaattgcg tatggcgctg tgggcgattg
      841 catgtgcagc tgcgttggcg cttttattag gatatttctt atctcggcag atcaccggcc
      901 cattaatgac aatcgctagc gaaacccaaa aattagccag tcgggatttt agcgtcaata
      961 tacaggtgca tactggcgat gagctacagg ccttggcaca gcacataaat gtgatagctg
     1021 gtgagattaa atattttaat cagcgccaga aacagtggtt aatggatatt tcccacgaat
     1081 tgcgtacgcc gattaccatt ttatatggag aggtaaccgc aattttagac aatgttacta
     1141 aagctgattc caacaccatt aaatccttgc aggaagaagt cgtacattta aaacgcctgg
     1201 ttgacgattt gcatgcacta tccgtattgg acgatggtga atttcactta tcctgtgagc
     1261 ccatagagtt gggtgaatta atccaacaac aggcgagccg ttatgcggag cgatttgaaa
     1321 ccctcggcct tgagctgcat atccactgcg ctggggaagt tgtttgggtt aatgctgacc
     1381 gggatcggct ggggcaagtt ttgcgcaatc tgctggagaa ttgcctgcgg tatacccacg
     1441 cacctggcgt agtgagttta aatttgcacg tgcacgccag cgcagtgcat ctaattttag
     1501 aggattcagg gccgggtgtg ccggcggaag tattggcgaa attatttgac cgcctttatc
     1561 gggtggacgc agcacgcacc cgcgctggcg gcggggcggg gctgggattg gccatttgca
     1621 aaaccatcat actggcccac gccggtagta tcgaggcatt tttaaccgag cagggtggct
     1681 taggggtgag aataatattg ccacgcacgc gagttgaagc tgtatgagcc agataatatt
     1741 agtggtggag gacgaaatta aaatcgccga attaatcggt aaatatttaa cccttgaggg
     1801 ctacgagccc atattggtcg ataatggcgt cgacgctttg gctattttta aacaacggca
     1861 accggccctg gtgattttag atgttatgtt gccaggtatg aatggtttga gtgtctgtga
     1921 gcgattgcgt gctgtatcgg atgtgccgat tattctgtta acggcgcggg tgcaagatgt
     1981 tgaccggctc atcggctttg ccagtggcgc cgacgactat gtgtgtaagc catttaatcc
     2041 gcaagaatta atggcgcgag tgcgggctat tttgcggcga ataaaaccag cggctgcacc
     2101 tactacgcta agttttgaca aaattaccat cgatcaggcg cagcacggtg tatgggtgag
     2161 tggcgtaaaa gtgcaactga cgcagattga atttaaattg ctgctacaga tgttggagca
     2221 gcccgccaaa gtatttagcc gtgaggagct gttgctttca agccgtggta aatacaccga
     2281 atcctacgag cgcaccattg atttccatgt gaaaaatctg cgcaaaaaaa ttaatatcga
     2341 cgcgggttcg aattacattc ataccgttta tggcttgggc tataaactca ttggataacc
     2401 tgctgccctt tattgccctg gcggcaaaat agattgtctt gatttgtttc accttcgctg
     2461 ggctaaaact agcgaaaagg tgcagaattt tttctggcgc cgctcgcggc ggcgctagca
     2521 ccgatataac aaccttaccg cgttttttta aaccgccacc aaatggtgct gggttaatag
     2581 caatgtggtt acggggctga tttcctattg ggccagaagg gcatcccttg cgggcgcgca
     2641 gtcgagcgta catcctgcca atacgcctag caattttggt gtgctgcttt tccctgcgca
     2701 aaggggcata ggtcttgcca atacagcctg tactcctcgc acattgcgcg gtaaggtgcc
     2761 gggcgtacaa gatgaggtat ttatgcgcac gggtttaatt aacagtatta cagaggttga
     2821 ggcgagcctt tggcagcagc tctgggccac tgactacccg tttatccaac acgattttat
     2881 ggccctgtta gaggacagca agtgcgtggg tggccgtagc ggttggacac cccggcatat
     2941 tctgatctgg caagaagaca ggctggtggc cgccatgccc gggtttatca aaacccattc
     3001 ctacggtgaa tatgtgttcg attggagctg ggccgatgct tacggccgcc acggcttgga
     3061 gtattacccc aaatggatca acgccatgcc cttcaccccc gccactggcc cgcgcctagc
     3121 catagctcca ggcgccgatg gggcggccgt tatgcaagcg cttaatgaag cctggcagga
     3181 gttgctaaaa cagggggcgc acagcctgca cagtttattt cccgataacg ccagcctgcc
     3241 gcgcttacct gccgcttggc acacccgctg gggctgccaa tatcactggt ttaacaatgg
     3301 ctttagggat tggcaggact ttttagccca gtgcaacagc cgcaaacgca aaatgatggt
     3361 gaaagagcgg caaaaggtgc aggcgttgaa tttaaccatc gcggttaaat ccggcacaga
     3421 attaacgggc gaggattggc aggcatttta tggtttgtat caaaacacct acgccaaacg
     3481 cagcggccgc ccaggttatt taacgcagga tttttttacc ggtttacagg ccattttgcc
     3541 cggccaaatc caccgtgtgg acgttagccg cagcggccaa ttaattgcgg cggcattata
     3601 ttttagcgat ggcgaaacac tttatgggcg ctattggggc tgcagcgagg aacacgacgg
     3661 cctgcatttt gaggcctgtt attaccaggg cattgaattg gcgattaaac tcggccttaa
     3721 acgctttgat cccggcgccc agggtgagca caaaattgcc cgcggcttta cgccggtatt
     3781 gacctgttcc aaacacctct tagcccatgc ggggtttcat gaggcggtgg gggaattttg
     3841 ccgggaggag cagatgggta ttgagcgcta tattcaatcc gcccggcaag cgctgccctt
     3901 taaggaaggt tatgtctgcg tggagccggg tattttgcaa aaaaataaat cggaaattat
     3961 ttattaacgt cctcctttaa aaagtgggaa tattctgcag tgggtgcgtt tgcaaagtgt
     4021 ttacgtatgc aggcgttatc agagcgctct caataaaagg tgttatgctg tgtcaattgc
     4081 attgctgcat gggatgctaa tgtatttaaa tcggcggcta ataatcgctt gttcaaataa
     4141 aaacgagaat atacatgcaa tcattattag gtcgatggtt agggttatta tgtttagctt
     4201 tagctgctta ttgttttatg caggtggggc agggattaat ggcaggggaa attactaaat
     4261 taagcaaata cagccaggaa attatccagc gcgcagcgca gcctgagcgt ttttggattg
     4321 ccatagggtt ttggactata ggagcaacta tattgctggt aacgggtatc aagaaaataa
     4381 cccgcaggga ttagcctatt gaacctaaaa ccgcagcagg gtttaactgc ggtttttagg
     4441 ttgaaatacc gccttgccag agccctctgt atttgaccgg gggtgcatga tcaagcgcgg
     4501 cctgcagaat aactcaagtg agctattata agaagacgcg aatagtgtga tgcgtttggc
     4561 gtcattttaa tagtgaatct gttggccctg caggcctgca atgagctttt attataagcg
     4621 ccagaggatt agggggtgcg aaggattctg agcagtacag atgaccggca ggctcggata
     4681 cttacggcat tatcactcat gccactatcc gcacttttta tatttttatt tcggtcagta
     4741 ttgttaccgc gtgtggcggt aaaactcata gatcccacct gttaagcgga gagcacaagc
     4801 cgcatcctgc actttttacg catctatttt ataacggctg ctaataccca tggctaagtg
     4861 cccatggtat tggctactgg aatggttgaa tcctaagggt aagtgctgct ttatgtctaa
     4921 cgatgatcac agcaaatttc ggctggtttt gtttacgccc taccatgaag gttttttcta
     4981 cggcgagatt attaaccacg tgcgcttgct gtgtaagtta aaaaattatg ccttggacgt
     5041 aattgccacc catagttttg gtgagtacca cagcaatttt tggctggcca atgtgggggc
     5101 tgttattatt gtgcgcaatg caatcagccc caattttgcc gcgcaattaa gtgcgcgcgg
     5161 tattccgtgt gtgtcaattg cctatgacta cttccccgcg cccattcata tggtgtgcag
     5221 cgataaccgc caaggcgtta agctggcctt tgaacactta attgcccaag ggcacaagcg
     5281 tattgctttt atcggtgact taacgcaata cgatctgcgc aaacgctatg agtcctattg
     5341 tgaggaacat agtcggcata acttgctgtt agatgaagag ctgtcttttt ttacgcaaaa
     5401 cactacttat ttgggcggct ttaacgccgc cacggaattt gtcaaacgcg actgtgatgc
     5461 caccgctgtt attgccgcgg ccggcttgtt ggcggaaggg tttaagtccc gtttaaatga
     5521 catcagccct atgctctatg aatccctaga aattgtcgcc tttgatgtaa tgccggtgca
     5581 gattttatcc aacaaatcta ctatggcgat tgatttaaac ctgatgttta tcgcccataa
     5641 ttgcatcacc atcatcgagc gtattcgcgc aggccatgat gtggagcatc aaaccaaaat
     5701 tgactgccgc ctagtaacac tgcccgcttt tggagtggcg gcgccagatg atttagcccc
     5761 ctgtttggaa gaccccagct ttaccaactc cccctatgta aaaagcttga ttaccaatat
     5821 gtatgagtgg tccatggcga ttgcgggcac tgggttgagt gatgtgatga gcctgtcgcc
     5881 actgtttgat caattattaa aaatggggtt gttgtttagg ctggtgaaag gtgcaggcgc
     5941 cgttgaatcc gcccgcttaa ttaaggtgtg caaacgtcgg gaaacacttt taatggacaa
     6001 atatgaccca gccaaccaat gtttatctgc ggatgtgcag aatcattttg cagaccattt
     6061 tgaacacgcc gaatacgaat taaccaccag tattcctatt gttattaatg gcaaactgtg
     6121 gggcatattt accgcctgtg gcagcaatga aattggtgat agccgcaata attatttggc
     6181 gcttttatgt tatttggatt tgtcgataca gtttttaact aaaaatatca tctcctcttt
     6241 agatgccgca gctgacaaaa atccagccac tgctgccccc gtggcggagc atggcggtgt
     6301 ggtgacatgg gattttgcca agggtgtggt ggaatggagc gatgaggcgc tagccttttt
     6361 aggcttaacc tccccattag agaaaaacat ctaccgctat atggagctgg atgatcgcgt
     6421 gcatgaagat gaccaaagcg ccttgcgcgc ggggctttat aaactgcatg aacaggctgt
     6481 gcccctgcac attaatgtgc gtatgaaatc aaaatcaggt gggtatttat taattcgtat
     6541 tgatgggcag gcagatgcgg cccgccaatt aatgctgttt gagctccggc aagagctggg
     6601 tgcattgtaa gcttatgcaa tcgctaatgg cagtattcgt gagttacccc tgggtgtggg
     6661 ccattttgtt tttggctacc ttgggggtgt tattgatctg cctagccaaa ttgcgccagc
     6721 agcagaagga tttagccgag gccaaacacg ccaacagtat tagcagtgaa gagcaggata
     6781 aaaatatcgt cctggcattt ttggtggatc agtttgatcg aaccaaacgc ctcaatgata
     6841 atgtgtattt tcgccacgat gaactcgacc gccacgcctg ctccctgcgc agcgcctatt
     6901 taaaaattga agaaaaaagc ctaaaaaata acgcagatca tcgtcgatat cacttggcta
     6961 ttaataagtc tttggtgaag ctgctaaaaa ttattcgcga tgatgcaatc aaaacgattg
     7021 acgtggaagg ggtgtcagat cgctcgctgc acgaggcatt ggacggctcc aaactctcgg
     7081 cgcaggaaaa aagtgagttg ctcaaccaga tagaagagtt ttgtgagcaa ttaaaatcag
     7141 gtgccatagg tgttgagttt agaaaaaagc gctacaaaca gcttttagcc atgattaaaa
     7201 acgttgaaaa taccgaagag cgcgtggcga tgaaaaaact gctgttgctg gcggaatcgg
     7261 cggacgcaag tttcgagcat tattcgaatt tatcggagtc gatctttaat gcgggctcgg
     7321 atgtggccgc actgcaaggt gtacttgatg gctctggtcc ggggctcagt agccaggtgc
     7381 aggcgcaatt agatgccctc ggcaaagaga atatgaagct ttattcccaa gtacaaatgc
     7441 tgcgccagtg gtctaaggaa aataaaaaat tggcttaccc agaaggggtg agcctcagct
     7501 cagccactat taatgatgtt tccgaagaaa ttattaataa acatgaggaa gagctcaaag
     7561 ccttaaaagc tacggtgaga aaccaaaagc tgactatttt tgaacttgaa aatagcttgg
     7621 atgaataccg taagtcagag catgccaaag gcgctaagtc tgactatgag caggagttcg
     7681 gcgcattaac tcgccggtta accgccacgg aagatctcat tagcgagcag gaaaaaaacg
     7741 ttggccgtat gcgcaccggg ctggaagcct tgaacggcac cgaggctata ggcgaagagc
     7801 gcgaaacact tatgcagtgt attgaaaagc tgaaaaaaga agtaacggaa gccaaagagg
     7861 cctataccag caaaaacgta gagtttgatt ttattagtga gctgatcgcg gtgggctccc
     7921 ttgaggactt ggctttgttg gtatttcaat atttttgtga ccaaaaatgc gacccgtttt
     7981 tgctgatcag tcataaaggc cgcaatatcg aaatgtctgc cacgggggca ctgtctgtaa
     8041 aggataaaac cattattaac gctatgatgc ccaatgacgc ctccaaggac gatgagcgct
     8101 tccagctgca gtttcgttac cacaattttg gcggcattct gcggtttttg ccgggcagca
     8161 cggaaaatta tgattcctgt gaggcgctgt taaaaatggc caaaattacc gatgctttta
     8221 ttgaaaaaat atccagcggg caaaatatgc gcgggctaaa aaagcagttg gagcagtgct
     8281 ctaatgacgt gaaacagttg gcgcagacgg ttgatatagg ctttgaacaa atatgccagg
     8341 cgcaccggca gttaatatcg aatcatataa cacgcgggca gaatctattg cgtgtcaaaa
     8401 gtgccaccgc cgcgcaaatt gcctgtttaa agcagatgga aaccgatatg ttagaggata
     8461 tggcgcccaa cggcagtgtg cgtataaaaa tgcgtaaaga gtttctggcg ctggttaaaa
     8521 aaatcgagca ataattataa acgtgcaggc tgggttgatg gcgtcattac cctgttctga
     8581 aggttttttg ctgctgccct catcaaggca atgtcattag gttaatggcc attaggttaa
     8641 ggattagctg ccatttttcg cagcctggat ggagcgcagc gctccatcca gactacccag
     8701 atagtcctcc cctaaatttg cgcccctatt cctcacctta agggtattgc cgccacaaca
     8761 taaaatgtgt tgctgctaaa ttcccactct agatggcaaa cggctttggc actcgctgct
     8821 ggaagtaggt aatgtagttt gtgcctctaa acctttggcg ctgtaccaaa tgcggtattc
     8881 atcattatta tcaaaccgca gggcttgata tagggtgcca tcaatggtgc ggctgccgac
     8941 ggatttcata atggcttggt attttgcgcc caggaattca aatgttaaca gtaggctgta
     9001 attgtgggtt ttgttttggt tatccatttg gctccaaaat cctttaatta actcaccccg
     9061 cgagttgccc ttgcccaggc tgccgtcggc cttggccttt ttaatgtatt tagcacaagc
     9121 ggtaaagcta cctgcactcg ctgcgccatc gcctgcaaaa tacaattcaa attctatatc
     9181 ttttagattg ttgggttttt ggcccaagtc catatcggct acatgcagtt gtttgccggc
     9241 gacggaatta aatggcacgg cactcgcgtc cacctggccc agatgtttga tgccttgggg
     9301 gttttttaca tcctcatcgg aaaacatatg ctcagtcaat tggctaatat cagcttgttc
     9361 gctgcccagg cccgccaata attcttgatt ggcggccgca ttatcgcgga aaaatttgaa
     9421 tgcgcggtcg gtggcgggat ctttttcttt ttcgttgacg gctttcacac ccaatacttc
     9481 aacggcgcgt gcaccggcgc gggcgtcttc ggcagtggta tttttaactg ggggtaatag
     9541 ctgattaatt aagggcgcaa gcgcttgtag gcgctccaca ataacatcga attttgcctc
     9601 gtcgccactt tgagaaggca gaggtaattc tttgtcttga tagcgttttt gcacggcttg
     9661 ctcggcattt ttagccacgc tttttaataa attggcgtcg gtgagcgctt catccagcga
     9721 gccgttgtag ccgctaaggg cccgtgcgag ttcttggtag gcatggctgg tgccatcgct
     9781 gggcatacca ttttcatcgc ccagcgcggt atacacggca ccaatt
//
LOCUS       DAOQGS010000087         9815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3227_length_9815_cov_5.066393, whole genome shotgun sequence.
ACCESSION   DAOQGS010000087 DAOQGS010000000
VERSION     DAOQGS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_9815_cov_5.066393"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKZ52_04910"
     CDS             <1..377
                     /locus_tag="PKZ52_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNG59144.1"
                     /translation="VELKVRTQNIGNALFGQSGGFFLMEASGQGQLAVSGFGSMFQLN
                     VEPGKDVIIDNAHVVCWDANLHYEISVTTGSGSGGGLGGMLGNLVNSATSGEGIVLRF
                     SGAGKVYICSRNRKSFIELFKP"
     gene            417..1010
                     /locus_tag="PKZ52_04915"
     CDS             417..1010
                     /locus_tag="PKZ52_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNG59145.1"
                     /translation="MSINLQKGQKISLDKEAGVALTKIIMGLGWDVVQPEKKGLFGFL
                     GGNKEVDIDLDASCILFDEQNNAVDAVWFRQLRSKDGSIQHTGDNRTGAGDGDDEQII
                     VNLTQVPANVKSLVFTVNSFTGQNFSQIANATVRLVNAANNTEVARFNLSGLGNYNAQ
                     IMAKLYRHNGEWKLHAIGETANGRTFNELMPAIAPHL"
     gene            1852..2877
                     /locus_tag="PKZ52_04920"
     CDS             1852..2877
                     /locus_tag="PKZ52_04920"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNG59146.1"
                     /translation="MTRVWFNKTFSSLAAALRLIRQGDSAQQYRILCSNTNRHAPAFL
                     AADEHFIEPKGLLGMEYAKWCLQFCAEQKIAIFVPGKEASVIANLHQEFLQVGTRVLS
                     TASSDNLRLINDKARFCQSLDLPLAPAAEFRAVETLAQFDAAFEELRSRHHKLCIKPS
                     SSVFGLGFAVLDEKRSSAELLLEGVPYHINLQDLRRGLEKMGAFKKMLVMEYLPGHEY
                     SVDCVADKGRLITAVPRRKSLISGRGQTIDLREDILTSAAKLAADYQLNGFFNAQFRE
                     RTQGPLGLLEINARLSGGVAMACLAGPNLPYIGIAGFDRGFDQIEIPPIRNGIRVMEI
                     AQATELL"
     gene            2874..4028
                     /locus_tag="PKZ52_04925"
     CDS             2874..4028
                     /locus_tag="PKZ52_04925"
                     /inference="COORDINATES: protein motif:HMM:NF026947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HNG59147.1"
                     /translation="MNAQSPAPLHISLPTGELQLTLTQGAGRLDELLGFGARANAKRG
                     FLFVSKILAKHWPVKPAVMRAVHDELAAQVPNLPGPIVFIAMAETGVGLGQGVFEAYK
                     RLQPERACLFVHSTRYPMEGRAVFEFAETHSHAPRQYLHLPTDDALQSLFRFARSLVL
                     IDDEASTGNTFVNLARACRRYCPDLSHVHLATAANFMSAAARAECPAAFELQVSMAAL
                     IEGQYEFTPNPIEVSAPAAQAAPCPQTCGAKAEFGRLGTERVLDVPHSLALQLSTDLE
                     PDAKILVLGTGEFMHWPFLLGQCLEDLGHAVWVQSTTRSPILTWGAVQHSLTFKDNYG
                     EGVPNYVYNLAPGLYDEILICHETPENDALRELAERLGGRLVDGSCTPSR"
     gene            4242..5015
                     /locus_tag="PKZ52_04930"
     CDS             4242..5015
                     /locus_tag="PKZ52_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59148.1"
                     /translation="MKTFLFTDLDDTLFQTLAKCPPGQELVAAAFLADGRANSFSTPR
                     QRALLELFAAQATLIPTTARDLAAYQRVTLPISFTSYSIINFGGLVLRPDGSLDAAWY
                     NLMQADMAKAKAGLQDIIARLDAYCLHQGLPNRARLISDAGIEFFALLKDPEKNLSRL
                     QALEEAVIQPWLSQDGKDFVLHTNGNNHAILPRTLDKSRAVAYVSDLLRAEHGEILTL
                     GMGDSRSDGPFLALCDYAITPNNSQLANSSFNQFDWVMP"
     gene            5012..6127
                     /locus_tag="PKZ52_04935"
     CDS             5012..6127
                     /locus_tag="PKZ52_04935"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF022649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HNG59149.1"
                     /translation="MSSPSYTPFSGSYLAEDVQFLVTLLPPQPVVTVAQKERLIQSGA
                     RHYSQMLSPEAPPSAAYQALFEAACALNAERMAEDCLTLAALIAARKPGPLNLLSLMR
                     GGTPLGVIVGRLLRHWLKRPAAHFSLSILRDKGLDTQALRHVLKQGAAPASLVFLDGW
                     TGKGVIAKELAKSVAAFNAAHGTAIDTGLYSLVDLAGVSLGAASTADYLLPSSILNAT
                     VSGLLSRSLLRADLAPDAWHGCVYYEQLKGADVSRAFVDLIYERALARAACLSPAAWA
                     ERAADTGHLAAVSQRFLQACQTTYGVRDINLIKPGIGEATRVLLRRAPRLIILRASRA
                     PEVAHLRLLAAEKAVPIIEDPHLPYQAVSLIKSSPDG"
     gene            6120..7025
                     /locus_tag="PKZ52_04940"
     CDS             6120..7025
                     /locus_tag="PKZ52_04940"
                     /inference="COORDINATES: protein motif:HMM:NF026954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HNG59150.1"
                     /translation="MAKITTLGASLYVPATHKSLAQIASGDLRSVIFCTEDAINGDEL
                     PAALANVQAVLPLLPSDGSRQRFVRVRNLDVLDGLLAMPGIERLSGFVLPKATRHNFP
                     AYFAAVRHTQHLLMPTLETVEVFDDQEMLLFRACLQQPEVQPRILALRIGGNDLLALL
                     GIRRPRTLSIYRTPLGLVIAKLATLFRPHGFALTAPVFEHLDNTGLLAQELEEDLAHG
                     MVGKTAIHPSQVALIDQHYRVPHTDIEMALRVMDAAAPAVFKMHDSMCEVATHSAWAR
                     GVVERAKIFGVQQEESSELKMNIQA"
     gene            complement(7268..7744)
                     /locus_tag="PKZ52_04945"
     CDS             complement(7268..7744)
                     /locus_tag="PKZ52_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HNG59151.1"
                     /translation="MYQPPADVLEKALGIALIAHAGQKDKAGAPYILHPLRLMMTADE
                     PDTQVTALLHDVLEDSQLTEEDLNQAGIPAHIAAAVASLTRQIDETYEEFIERIAQDP
                     LARRVKILDIQDNLNLKRLKSVNKADLMRVTEYHRALQYLLEVEAQEAEMEDDLDD"
     gene            8024..9790
                     /locus_tag="PKZ52_04950"
     CDS             8024..9790
                     /locus_tag="PKZ52_04950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG59152.1"
                     /translation="MLSPGALAQALPQVAYLPDDGFSLDTIALTPTERWQPLAAGHLS
                     LGFNPRPHWLRIQLKASPTAAFLQLAYPHLDYVRLYQVHNGQLVKQVSTGDMLPFAMR
                     PLAHRYFVFPIAASSDPQTLYLWVQTSGLLQVPLKLVSREELAQGDQLYLLTQGLYYG
                     FFLAASLFAFFLCLAHRDTTYGLFSLLVLAYAGVEALYSGLGYGYLWPNLPALNQMTM
                     PICLSFAVAMAARFSLRVFRLQAGQRGYGAGQGLFWGALLLMALTPLLPVARALQACL
                     LLIGLHCCLQTGLGLYWLRRKQAGGGWYCASWAIFSLGTLAAALNYWGLLADGTSMFG
                     LNQAASLLQVVLLTFMVCYRVKIDYSNRLSAEFAARAEQSRAIEQEQAQAQQLAKAVE
                     EKTAALQAALCEVSKLNMELSKQTITDSLTGLFNRRHFDHLLDSEVRRAAREHRPISL
                     VLIDIDHFKKINDTYGHPVGDDCLIQLARLLRQVVRQPPDIVFRFGGEEMALLLPGSD
                     AAGAQVVAEKICSLVAASEFCYPAGCLQLTLSLGVGSLIPSQNTQPREVFELADEALY
                     LAKNRGRNRVACLTHSDRIAAN"
BASE COUNT         2059 a         2949 c         2656 g         2151 t
ORIGIN      
        1 gggtggaatt aaaagtgcgc acgcaaaata tcggcaatgc ccttttcggc caatccggcg
       61 gcttttttct catggaagcc agcggccagg gccaattggc ggtgtccggt ttcggctcca
      121 tgtttcaatt aaatgtggaa ccgggcaaag atgtcattat tgataacgcc cacgtggtgt
      181 gttgggatgc caatttacat tatgaaattt ccgtcactac cggaagcggt agcggcggcg
      241 gtttaggcgg tatgctgggc aacttagtca acagcgctac cagcggcgaa ggcatagtgc
      301 tgcgctttag cggcgctggc aaagtgtata tctgctcgcg caaccgcaaa tcatttatcg
      361 aattatttaa accctaatat cctacccaac accccttaat tttacggagc aaccctatgt
      421 ccattaattt gcaaaaaggt caaaaaattt ccctcgataa agaagcgggc gtcgccctca
      481 ctaaaattat catgggcctg ggctgggacg tggtacagcc agagaaaaaa ggcctctttg
      541 gctttttagg cggcaacaaa gaagtggata tcgacctgga tgcctcctgc attttatttg
      601 atgaacaaaa caacgccgta gacgccgtgt ggttccgcca actgcgctcc aaagacggct
      661 ccattcaaca cactggcgac aaccgtaccg gcgccgggga cggcgatgac gaacaaatta
      721 ttgttaacct cacccaagtg cccgccaatg taaaaagcct ggtttttacc gttaacagct
      781 ttactggcca gaatttctcg caaatcgcca acgccaccgt gcgtttagtt aacgccgcca
      841 acaatactga agtggcgcgc tttaatttat cgggcctggg caattacaac gcgcaaatta
      901 tggcgaagct ttaccgccac aacggcgagt ggaaattaca cgccataggt gaaactgcca
      961 atggccgcac ctttaatgaa ttaatgcctg cgattgcacc gcatctttaa tcgcaccgca
     1021 tctttaaatc acagcccaaa taaatacgct ccggcattgg tgcattgccc ggagcgttta
     1081 tcgaggctgc gttttatgca cccggcatgc tcaacctaaa aaccgcagtt gaactctact
     1141 acggccgcct aatgcttgaa tctgcacgct tttttgtcaa aatttgactc accgtagtgg
     1201 tgactactgc tttcgccaaa ttttgacaaa aaagcgccca tattcagcgc cttaggcacc
     1261 ctcgctacga gttcaactgc ggtttttagg ctcaaacctc gccgacgatt acctctgcat
     1321 gatgattaac gccgccacgg gcaaccctaa ggaaatcaca tgaattatga cgcgcgtagc
     1381 caattggctt tggaggagct acacaatacc gggatcactc aatcgagggc ccttcctcca
     1441 ctgtggaaat tggcaacccg ctgtggcttg cagccgcgcc cgccccatta caatagtttt
     1501 ttacacaaca ctttatcctt tgggatttat tttggggttt tctggggcac aactatgtat
     1561 ttattggtct ggcgcactca accaggctct tggcttgtat attcctctct cgcactgctc
     1621 gcgggagctt tatttgggct ctgtatggcc acgcattttc gctatggttt taaaaaacac
     1681 aaactaacgc catgggaaca gctcggcgcc aaaaacgtgg gccaggccga cactgcaacc
     1741 cactaattac gacgggctca ataacagcac tgatacctac ctgcactaca ctcccggcaa
     1801 tcatttggcc gaacactttt agatgtaccg cattttcatg agacacctta aatgacccga
     1861 gtctggttca acaaaacctt ttcctctttg gccgccgccc tgcggttaat tcgccagggc
     1921 gacagcgccc agcagtaccg cattttatgc agtaacacca atcgccatgc cccggccttt
     1981 ttagccgcag atgaacattt tattgagcct aagggtttat tggggatgga atatgctaag
     2041 tggtgtttgc aattttgcgc tgagcaaaaa attgccattt ttgtccctgg caaagaggcc
     2101 agtgtaatcg ccaatctgca ccaggaattt ttgcaggtgg gcacgcgggt tttatccacc
     2161 gccagtagcg ataatttgcg cttaattaat gacaaagccc gcttttgcca aagccttgat
     2221 ttgccgctcg cgcccgcagc ggaatttcgc gcggtggaga cgctcgcgca atttgatgcg
     2281 gcgtttgaag agttgcgcag ccgccatcac aaactctgta ttaaaccctc atcctccgta
     2341 tttggcttag gctttgccgt attggatgaa aaacgctcca gcgctgaatt gttattagaa
     2401 ggtgtgccct accacatcaa cttgcaggac ctgcgccggg gtttggaaaa aatgggcgca
     2461 tttaaaaaaa tgctggtgat ggaatattta cccggccacg aatacagcgt ggattgtgtg
     2521 gcggataaag gccggttaat taccgccgtg cccaggcgta aatcgcttat ttctggccgc
     2581 gggcaaacca tcgacctgcg cgaggatatt ttaaccagcg ctgccaaact cgccgccgat
     2641 tatcaattaa acggcttttt taacgcccaa tttcgcgagc gtacacaagg gccgctgggg
     2701 ctgctggaaa taaatgcccg cttatccggc ggcgtggcta tggcctgttt agcaggcccc
     2761 aatttgcctt acatcggcat tgccgggttt gatcgcggct ttgatcagat cgaaatcccg
     2821 cctattcgca acggtattcg cgttatggaa attgcccaag ccacggagct tttgtgaacg
     2881 ctcagagccc cgcgccccta cacatcagcc tgcccaccgg tgaattgcag ctaacactta
     2941 cccagggcgc cgggcgcctg gacgaactat tgggttttgg cgcccgcgcc aatgccaagc
     3001 gggggttttt gtttgtcagt aaaattctgg ccaaacactg gccggtaaaa cctgccgtga
     3061 tgcgcgccgt gcacgatgaa ctggccgccc aggttcctaa cctgccgggc cccattgtgt
     3121 ttatcgccat ggcggaaacc ggcgtgggat tgggccaggg ggtatttgaa gcctataaac
     3181 gcctgcagcc tgagcgcgcc tgcctatttg tgcacagcac ccgctacccc atggaaggca
     3241 gggcagtgtt tgaatttgcc gaaacccaca gccatgcgcc gcgccagtat ttgcacctac
     3301 ccacagatga cgcccttcaa agcctgtttc gctttgcccg cagcctggtg ctaatcgacg
     3361 atgaggccag caccggcaac acgtttgtaa acctcgcccg cgcctgccgc cgctattgcc
     3421 cggatctaag ccatgtacac ctggccacgg cggcaaattt tatgtccgct gcggcccgcg
     3481 ccgaatgccc agctgctttc gagctgcagg taagcatggc ggcgctgatt gaggggcagt
     3541 atgaattcac ccccaacccc atagaggtga gcgcccctgc cgcccaggcc gccccttgcc
     3601 cccagacctg cggggcgaag gcggagtttg gccgcttggg caccgagcgg gttctcgacg
     3661 tgccccacag cctggccttg caactgagca ctgatcttga gccggacgcc aagatactgg
     3721 tgctgggcac tggcgaattt atgcactggc cttttttgct cggccagtgt ttagaggacc
     3781 taggccatgc cgtctgggtg caatccacca cccgctcccc catcctcacc tggggcgccg
     3841 tgcaacacag cctcaccttt aaggataact acggcgaagg ggtgcccaat tatgtgtata
     3901 acctggcgcc gggactctat gacgaaatac tgatttgcca cgaaacccca gagaatgacg
     3961 ccctgcggga gttggcggaa cggctggggg ggcgtttagt tgatggttca tgtacaccta
     4021 gccggtaaag ggcaaggtca ctgggttggg gccgacaggg ctatgccatg gggcttcctc
     4081 ctggagcctc ggctatgaat caggatttac ccgggcaccc acaaggggcg cccctacggc
     4141 cgttggttag cgaggacatc cttagttcca acattgtgta aaaaggcaat aacacaggct
     4201 cattgccctc ataataataa gcccagcctg cgagattcgc tgtgaagaca tttttattta
     4261 ccgacctgga cgacaccctc ttccaaaccc tcgccaaatg cccgccgggg caggagttgg
     4321 tggcggcggc ctttttagcc gacggccgcg ccaattcctt tagcaccccc cgccagcgcg
     4381 cgctgcttga gctcttcgcc gcccaggcca cccttatccc caccaccgca cgagacctgg
     4441 ccgcgtacca aagggtaacc ctgcccatca gctttaccag ctacagcatt atcaacttcg
     4501 gcggcctggt gttaaggccc gatggcagcc tagatgccgc ttggtacaac ctcatgcagg
     4561 cggatatggc caaggccaag gcgggtttgc aggacatcat cgcccgctta gatgcctatt
     4621 gcctgcacca gggcctgccc aaccgcgctc gcttgattag cgacgccggt atcgaatttt
     4681 ttgctctgtt aaaagacccg gagaaaaacc taagccgcct gcaggcccta gaagaggcgg
     4741 tgattcagcc ctggctgagc caggacggga aggattttgt cttgcacacc aacggcaata
     4801 accacgcaat tctgcctaga accttggata agtcccgcgc cgtggcctat gtgagcgatt
     4861 tattaagggc tgaacacggt gagattttga ccttaggtat gggcgatagc cgctcggacg
     4921 ggccgttttt agccctctgc gattacgcca tcaccccaaa caacagccag ttggccaaca
     4981 gcagttttaa ccagttcgat tgggtgatgc cgtgagcagc cccagctaca ccccctttag
     5041 tggcagttat ttagcggagg atgtgcagtt tctcgtcacc ctgctgccgc cccagcctgt
     5101 ggtgacggtt gcccaaaagg agcggctgat tcagtccggc gcccgccatt acagccaaat
     5161 gttatcccca gaggcgccgc cctccgccgc ctaccaagcc ctcttcgaag cggcctgcgc
     5221 cctgaatgct gagcgcatgg ccgaggattg tttaaccctt gccgccctaa ttgccgcgcg
     5281 caaaccgggg ccgcttaacc tgctctccct catgcgcggc ggcacgcccc taggcgttat
     5341 tgttggccgc ctgctgcgcc attggcttaa gcgcccggct gcgcatttca gcctatccat
     5401 cctgcgcgat aaaggcctgg acacccaggc gctgcgccat gtgctcaagc agggcgcggc
     5461 ccctgcatcc ctagtatttt tggacggctg gaccggcaaa ggcgttattg ctaaggagct
     5521 ggcaaaatcc gttgccgcat ttaatgccgc ccatggcacc gccatagaca ctggcctcta
     5581 cagcctggtg gatttagcag gggtgagcct gggcgccgca agcacggccg attacctgct
     5641 gccgtcgtcg atcctaaacg ccactgtatc cggcctgctc agccgctccc tgctcagagc
     5701 cgatctagcc cccgatgcct ggcacggctg cgtgtactat gagcagctga agggcgctga
     5761 tgtctcgcgt gcgtttgtgg acttaatcta tgagcgcgcc ctcgcccgcg ccgcctgcct
     5821 aagccccgct gcctgggccg agcgcgctgc cgataccggg catttagcgg ccgtttccca
     5881 gcggttttta caggcctgcc aaacaaccta tggcgtgcgt gatataaacc tgatcaaacc
     5941 cggcataggc gaggccaccc gagtactgct gcggcgcgca cccaggctga ttatcctgcg
     6001 ggcgagccgc gcgcctgagg tagctcacct gcgtctgttg gcggctgaaa aagccgtgcc
     6061 gatcattgaa gacccccatt taccttacca agccgtatcc ttaattaaga gttcccccga
     6121 tggctaagat caccacccta ggcgcctcct tgtatgtgcc cgccacccat aagagccttg
     6181 cccagatcgc cagcggcgat ctgcgctcgg tgattttctg cactgaagac gccatcaatg
     6241 gcgacgaatt gccagccgcc ctcgccaatg tgcaggccgt gctgcccctg ttgcccagcg
     6301 acggcagccg ccagcgcttt gtgcgggtgc gcaatttgga cgtgttagat ggactcttgg
     6361 ccatgcccgg catcgagcgc ttgagcggct ttgtgctgcc caaagccacc cgccacaact
     6421 tcccggccta ctttgccgcc gtgcgccaca cccagcacct attgatgccc accctggaga
     6481 cggtggaggt gtttgacgac caggaaatgc tgttatttcg cgcctgtttg cagcagccag
     6541 aagtgcagcc gcgcattctc gccctgcgca tcggcggcaa tgacctgctc gccctcctgg
     6601 gcattcgccg cccgcgcact ttgagtatct accgcacccc cttgggcctg gtgattgcca
     6661 aactcgccac cctgtttcgc ccccacggct ttgccctcac cgcccccgtg tttgagcacc
     6721 tggataacac cggcttactc gcccaagagc tagaggagga tttggcccac ggcatggtgg
     6781 gcaaaaccgc catacacccc agccaagtgg ctcttatcga ccagcactac cgagtgcccc
     6841 acacggatat cgaaatggct ctgcgggtga tggatgcggc ggcgccggcg gtttttaaaa
     6901 tgcacgattc catgtgtgaa gtggccaccc acagcgcctg ggccaggggt gtggtggagc
     6961 gagcgaagat ttttggtgtg cagcaggaag agagcagcga gttaaaaatg aatattcagg
     7021 cttagattgg gcggtttttt gcccccgacc ggcaagacgc agacggagcg cgctgggccg
     7081 agcaagctgg actgagaagg ctcggctagg tgcaccgcgc cgaactaggg agcctttaga
     7141 aaaatgcagg cggcgaagca ctaactaggc gaatttggca aaaaagcgcc gtttacatgg
     7201 agtaaatgag cattttttgc caaattcaac gacgctaggg cgagtgaggt aattgttcag
     7261 aggtccccta gtcatccagg tcatcctcca tctccgcctc ctgggcctcc acctccaaca
     7321 ggtactgcag ggcgcggtgg tattcagtaa cgcgcataag atccgccttg ttgacggact
     7381 ttaagcgctt gagattgagg ttgtcttgaa tatcgagaat tttcacccgg cgggccaagg
     7441 ggtcctgggc gatgcgctcg ataaattcct cataggtttc atcaatttgc cgggtgaggg
     7501 aggccacggc agcggcgata tgagcgggga tgcccgcctg attgagatcc tcctcggtca
     7561 gctgtgagtc ctccaacaca tcgtgcaaca gggcggtcac ttgagtgtcc ggctcatctg
     7621 ctgtcatcat gagccgcagg gggtgcaaaa tgtagggcgc gcccgccttg tccttctgac
     7681 ctgcgtgggc gatcagcgcg atgcccaaag ctttctctaa aacatcggca ggtggttggt
     7741 acataaaaac tccccaggtg taggtcgtgc cgttcaggcg cggcagtata tagacccgat
     7801 ggaataaatt cacttcgcag gcaagccgac actcagggag cggcagtcta tgcaacctta
     7861 gtctggcaga cgtgatccag ctaacaataa aggcttagtt tcgaactgtt tagggcggcc
     7921 aaatggcgta tattgccgcc ccgaatccaa cacgccaggt gagttgtcga tggactttgg
     7981 ggccgcccta aaacactgtt tgtgccttgt gctctgctgg cttttgctaa gccctggggc
     8041 cctggctcag gcattgcccc aggtggccta tctgcccgac gacggcttca gcctcgacac
     8101 catcgccctc acccccaccg aacgctggca gcccctcgcc gcaggtcacc tctccttagg
     8161 ctttaacccg cgcccccact ggctgcgcat ccagcttaag gccagcccaa cagcggcatt
     8221 tttgcagctg gcctaccccc atctggatta tgtgcggctg taccaagtgc acaacggcca
     8281 gctggttaaa caggtgagca ccggcgacat gctgcccttt gccatgcggc ccctggccca
     8341 ccgctatttc gtgttcccca tagccgccag cagcgacccg caaacgctgt acctctgggt
     8401 gcaaaccagc ggcctcttgc aagtacccct aaaactggtg tcccgggagg agctggccca
     8461 gggagatcaa ctctacctgc tcactcaggg cctttattac gggtttttcc tggcggccag
     8521 cctgtttgcg tttttcctct gcctggccca ccgggatacc acctatggcc tgtttagcct
     8581 gctggtgctg gcctacgcag gggttgaggc gctctacagc ggcttaggct acggctacct
     8641 ctggcccaat ctgcccgccc tcaaccaaat gaccatgccc atttgcttaa gctttgccgt
     8701 cgccatggcg gcgcgctttt ccctgcgggt gtttcggtta caggcggggc agcgcggcta
     8761 tggcgcaggc cagggcctat tttggggcgc cctgctgttg atggccctca cgcccctgct
     8821 gccggtggcc cgagcgttac aggcctgcct gctgctgatc ggcctgcact gctgcttgca
     8881 aaccggcctc ggcctgtatt ggctgcgccg caagcaagcg ggcggcggct ggtattgcgc
     8941 cagctgggcg attttctccc tcggcactct ggccgccgcc ctcaactact gggggctgct
     9001 ggccgatggc acctccatgt tcggcctcaa tcaagcggcg agcctgctgc aggtagtgct
     9061 gctcaccttt atggtgtgtt accgggtgaa aattgattac tccaaccgcc taagcgccga
     9121 atttgccgcc cgcgccgagc agagccgcgc catcgagcaa gaacaggccc aggcccagca
     9181 gttagccaaa gccgtggaag aaaaaaccgc cgccctgcaa gccgccctgt gcgaggtgag
     9241 caagctgaac atggaactct ccaaacaaac catcaccgac agcctcaccg gcctgttcaa
     9301 ccggcggcat tttgaccacc tgctggacag cgaagtgcgc cgcgccgcga gagagcacag
     9361 gccgatcagt ttggtgctca tcgacataga tcactttaaa aaaatcaacg acacctacgg
     9421 ccacccggtg ggggacgact gcctgattca gctggcccgc ctgctgcgcc aggtggtacg
     9481 gcagccgccg gatatagtct ttcgcttcgg aggggaggaa atggcactct tgttgcctgg
     9541 ctctgacgct gcaggcgcgc aggtagtggc ggaaaaaatc tgcagtttgg tggcggcgag
     9601 tgagttttgt taccccgccg gatgccttca gttgaccctg agcctaggcg taggcagcct
     9661 cattcccagc caaaacacac agcctcggga ggtgtttgaa ttggcggatg aagcgctcta
     9721 cctggccaaa aatcggggtc gcaaccgcgt agcgtgttta acccacagcg atagaatagc
     9781 ggcgaattag gctggcgatt ttggcgccgc tcgcg
//
LOCUS       DAOQGS010000088         9759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3255_length_9759_cov_4.512263, whole genome shotgun sequence.
ACCESSION   DAOQGS010000088 DAOQGS010000000
VERSION     DAOQGS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9759
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3255_length_9759_cov_4.512263"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /gene="rplM"
                     /locus_tag="PKZ52_04955"
     CDS             complement(<1..97)
                     /gene="rplM"
                     /locus_tag="PKZ52_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007737.1"
                     /note="in Escherichia coli this protein is one of the
                     earliest assembly proteins in the large subunit; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNG59153.1"
                     /translation="MKTISANAQTVQREWYILDASGKTLGRIAAEI"
     gene            complement(241..1359)
                     /gene="zapE"
                     /locus_tag="PKZ52_04960"
     CDS             complement(241..1359)
                     /gene="zapE"
                     /locus_tag="PKZ52_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNG59154.1"
                     /translation="MSSSPTAPSLTPLARYQQDAQRPGFIADASQAAAITALNDLFVR
                     LVNAQATRQSLWHSLFSSGKAKEPPIGVYFWGGVGRGKTYLMDVFFDALPFERKMRTH
                     FHRFMRRVHAELTELKGQKNPLEQVADRLAKETCVICFDEFFVTDITDAMLLSGLLSA
                     LFKRGVCLVATSNIVPDGLYPNGLQREKFLPAIALLNRYTQVINVDGGIDYRLRTLQQ
                     AALYFYPLSEQSQLELANHFQRLSHGAPQQPVTLTIEGRQVMALSEWEDVVWFEFKAI
                     CEGPRSQQDYIVLAKEYHTILLSGVPQLGAANDDAARRFVYLVDEFYDRGVKLIISAA
                     EPIASLYTSGKLSFEFARTQSRLLEMQSEEYLASPHKP"
     gene            1490..1963
                     /locus_tag="PKZ52_04965"
     CDS             1490..1963
                     /locus_tag="PKZ52_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HNG59155.1"
                     /translation="MYTLELVVIISLAALLFGLGLGVLLGRLKFGQSSSNQLVNRLQQ
                     AEAELENYQHQVAKHFVDTSQRISDLTQSYRDLHQHLADGAARLASPDISRKLLEAAE
                     AKNLAAEVIDQSAVSLEPPKDWAPKIPGQKGGLSDEFEDDDKKAPPVFTPPNYQP"
     gene            1975..3159
                     /locus_tag="PKZ52_04970"
     CDS             1975..3159
                     /locus_tag="PKZ52_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNG59156.1"
                     /translation="MRKALEYLVWPLLTGLLVAVIALVLFPQLRSPQTVVEPTALAPQ
                     NFAAGFSGPVSYAEAVKRAAPAVVNIYTRTKIQERRRRLIEDPLYRHFFNSADLGRQE
                     RMQSALGSGVIVSESGYLLTNIHVIRGADEVVVQLQDGRETQATLVGVDEENDLAVLH
                     IKADKLTAIHIGAPRNAQVGDVVLAIGNPFGMGQTVTQGIISATGRHGLGISTLENFI
                     QTDAAINPGNSGGALIDTQGNLLGINTAILDRLGNSSLDGVGLAVPADTALLSLKDIV
                     EMGKVVRGWLGVEAQPVTPLIAQQLNLQALNGVVVTAIFNNGPAHKAGLTPGDIILKV
                     NGQAVGNGTLGMQEILKARPGQAMAIEIWRQGELKTLNAVLGSKPDLKEQEGAALNAN
                     KE"
     gene            complement(3211..3588)
                     /gene="fliS"
                     /locus_tag="PKZ52_04975"
     CDS             complement(3211..3588)
                     /gene="fliS"
                     /locus_tag="PKZ52_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNG59157.1"
                     /translation="MNTQAALKSYAKVQYRSNVDVASPHKLIDMLFDGAIERIAQAKG
                     AMQFGDIALKGQKISSAISVVGGLRESLNTEAGGELAHNLDNLYVYIQRILTNAHSKN
                     DIKLLDEAATLLGELQSAWKQIG"
     gene            complement(3660..6683)
                     /locus_tag="PKZ52_04980"
     CDS             complement(3660..6683)
                     /locus_tag="PKZ52_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59158.1"
                     /translation="MRGLSGVSFLVCVVWLLCSPCSLAATCASLFPDGLQSHTASTGR
                     ITLNSGAVVNNSGSVLTGPLITNNAGSGSCGTGITCTISGAASPAQSMTISGGTGASG
                     AINVTSGTPSFAAGNYTSLSISNTYSLTFSTSGGNYYFTGDWTLGDSTTITFAPGNYY
                     INGNIYFNGTGSKQLRIGSTGTVNIFVNGQVVMGGVVTTPSVSSRYLLIYANGSIDVN
                     GASTIHAFLYSHNSTVTLNSTAVVYGGLSGSNVTLNGAQTVYYESTEISNAAFGSFCT
                     ASAASLNNFLIDVGGGTGSNCAASTINITARDSSNATLTTYAGTVTISTSTANGDWAK
                     TATAADAYGTLTAGATDSGAATYVFNTSDAGTISINLTDTHAESLTISLNDSSAGVTS
                     TSSTLAFSRNAFVVTSTDSLASDIIAGRTHSLRVTMMKQDTGGTSCSAASDYNVSGVK
                     AWLTRASSDPAGTAPNLINSSSASVALPNAKPGSNNVTLNFSSGIANFTLSTSDVGKY
                     ALNFADSSNSYASSEITGSSSTYVVRPFGFYIAVTSNPAASSDSGSVFTTAGTNFTVS
                     VTAKAYSAADDANSDGLPDNHADTNAANNANLADNTTLTKFGAETPAETITLAAALRL
                     PSGGVDPGLSDGDATASDGRVISSFSSGVGSTTQVYFGEVGIIEIAASITDGDYLGAG
                     TTPTTNMVSRSGYVGRFKPYQFAASSVSMTSFCSASTAFNYLGKDFTANLTLAAQNQQ
                     ASTTQNYTSTFVKLTSAGYSLKARDTTSATQLTSRMQLSSLSGTWSSGQVAASVSMNM
                     ARSSAPDGPYTTTKVGVTISDTDSVSLRSADITFDSDANGSNDALLFGQSAFYFGRLR
                     LDDAFGPETANLNVNFQTDYWTGSIWSRNTADSCTTIATSAITYPFGAINIAANRSVT
                     IGAGSTTGNYTSLAGSAVNFSSGDAGHYFSAPGSGKTGSFLVDVDLTSYAWLRFDWNN
                     DANYSNDTALPSARYTFGTYRGNDRTLFWQDSSVP"
     gene            complement(6683..7123)
                     /locus_tag="PKZ52_04985"
     CDS             complement(6683..7123)
                     /locus_tag="PKZ52_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshP"
                     /protein_id="HNG59159.1"
                     /translation="MCPRAYSTVNQQAGFLIPTALFIVVALGALAIAIARMAGSANSM
                     ALREALSAQAFFAAESGVQLGLYNLYNGATTRAQTTANCTALDVASQSFTAAGLKACS
                     VSFDCATSTDAGNTRSFYLITATASCGSGDLATSRVLNVKASMQ"
     gene            complement(7113..7940)
                     /locus_tag="PKZ52_04990"
     CDS             complement(7113..7940)
                     /locus_tag="PKZ52_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59160.1"
                     /translation="MPTNLISSRRFAAVGERKGRQRGFTLVELIFVLVILGLVGAIGS
                     SFLVDTMDMYQRTTNRVKVMQKGRVAIEQMSRYLRAAVPNSVRISSTGKCLEFVPVVA
                     GGYYMNQLADSENGGTASSSVVIAPLSYSGSAATQLIVAPLSSADIYTTASTSSRATV
                     SAVGANSITLSGAKLFTRNSPARRYYLASNPKRFCLLSGSLYRYSGYGFLTAALDDSN
                     PGGSADLMLNQLNQSASNFSLSAGSEQRNTSIGIQLSLTAGSETVVLRHQVLVRNVP"
     gene            complement(7918..8394)
                     /locus_tag="PKZ52_04995"
     CDS             complement(7918..8394)
                     /locus_tag="PKZ52_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59161.1"
                     /translation="MGCNSAHKQLIYRQTRGFTLVELLFFLVVVAIGLGGLLKVFNDA
                     VLHSVDPVLRVKAVEKTQALLDEILARKFAENTPTGGYPPCTSCTAISVDSGYDDVGD
                     YNGYSDSSDTGLAVSVTVVDAGSEIGVTNTNARRITVTTLMSDGSKVVLSAYKLNF"
     gene            complement(8677..9132)
                     /locus_tag="PKZ52_05000"
     CDS             complement(8677..9132)
                     /locus_tag="PKZ52_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59162.1"
                     /translation="MGMRGDYCNSAGFTLIELIAVLVIVGVVSVAAVGKFMSPDLYAL
                     QAARDQTLLCIQAAQQLAMTQSRSVRVLLSGTTLDVRKDANNDGVFAGGESVVLGAVT
                     YPISLSQGVSVSTSTLNFNSLGEVAASTITLTQQSRTLTINVSATGFAR"
     gene            complement(9273..9677)
                     /locus_tag="PKZ52_05005"
     CDS             complement(9273..9677)
                     /locus_tag="PKZ52_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNG59163.1"
                     /translation="MGKQQSGFTLIELIAVIVILGILAATAVPRFVNLQGEAATAATR
                     GVAGSIESASALNHAVDVAVAAGLTDTTTDPFYNVDNCQDGALLLQNGALPSGYSIAS
                     AAVADGATVTCTLTGPSSTTATFTIIGAAGGL"
BASE COUNT         2538 a         2537 c         2449 g         2235 t
ORIGIN      
        1 caatctcagc ggcgatacgg cctagagttt tgcctgaagc gtcgaggatg taccattcac
       61 gttgtactgt ttgagcattg gcgctaatag tcttcatgtt gttggccttt aacgtaggta
      121 ctaaaacccc caaaattacc tttggggagg tggagccggg gcggcaaatc gccaccccaa
      181 aaaaagagcg cggatactat aggagaaatt ctcccatatc aagtaaaaga gtggtggctg
      241 ttagggttta tgagggctgg cgagatattc ctcactctgc atttccagca aacggctttg
      301 ggtgcgggca aattcaaagc tcaatttgcc gctggtatat aaagaagcaa taggctctgc
      361 ggcggagata atcagtttta ccccacggtc gtagaattca tcaaccaagt atacaaaacg
      421 cctagcggcg tcatcgttag ccgcccccaa ctggggaacg cccgagagca agatggtgtg
      481 atactccttg gcgagcacta tgtaatcctg ttgggagcga gggccttcgc aaatagcttt
      541 aaattcaaac cagaccacat cctcccattc actcagcgcc attacctgcc gcccttcaat
      601 cgtaagggtt acgggttgtt gaggcgcgcc gtgggatagg cgctggaagt gattggccag
      661 ctccaattgg gattgttcgc tgagaggata aaaatacagt gccgcctgtt gcagtgtgcg
      721 cagccgataa tcgatgccgc catccacatt aataacctgg gtatagcgat ttaacagcgc
      781 aatggccggt agaaacttct cccgttgcaa gccgttaggg tagagcccat cgggcactat
      841 gttggaggtc gccaccagac acaccccgcg tttaaacagc gccgataaca accccgaaag
      901 cagcattgca tcggtaatat cggtaacaaa aaattcatca aaacaaataa cgcaggtttc
      961 cttggcgagg cgatcggcca cttgctccag gggatttttt tgccctttaa gctcagtaag
     1021 ttcggcgtgg acccggcgca taaagcggtg gaagtgggtg cgcatcttgc gctcaaaggg
     1081 cagcgcatca aagaacacat ccatcaaata ggttttgcct cgccctaccc caccccaaaa
     1141 ataaacacct ataggaggct cttttgcctt gccggaggag aataggctgt gccagagcga
     1201 ttggcgtgta gcctgggcgt tgacgagcct gacaaataaa tcattcaagg ctgtaattgc
     1261 tgctgcctgt gaggcatcgg cgataaaacc cggccgttgg gcgtcctgct gatagcgggc
     1321 taatggcgta agtgaggggg cagtgggcga gctggacatc ggcgggataa ccacttaaaa
     1381 agtcggcaac tttacaaagc ccctcgcacc tgtgcaattg atattggcca cccaccgcgg
     1441 caaatacagt atcatgcccc ttcttatctc tttgttttag gagccatttg tgtacaccct
     1501 cgaactggtt gtgattattt ccttggcggc gctgttgttc ggacttgggc tgggtgttct
     1561 gcttgggcgc ctgaaattcg gccaaagcag cagcaatcaa ctggtcaacc gcctgcaaca
     1621 ggccgaagcg gaactggaga attatcagca ccaagtggcc aagcattttg tggatacctc
     1681 acaacgcatc agcgacttaa cccagagtta ccgcgattta catcaacact tggccgacgg
     1741 cgccgcccgc ctagcaagcc ccgatattag ccgcaaactc ttagaagccg ccgaagcgaa
     1801 aaacctggcg gcagaggtaa tcgatcaatc cgccgtaagc cttgagccgc ctaaagactg
     1861 ggcacccaaa atacccggcc aaaaaggcgg cctaagcgat gagtttgaag acgacgacaa
     1921 aaaagccccc cctgttttta cccccccaaa ttaccagcct taagcgcaag ccccgtgcga
     1981 aaagcgcttg aatatctggt gtggccgctg cttaccggcc tgttggtagc ggtgattgcc
     2041 ctggtgttat ttccgcaatt gcgctcaccc caaactgtgg ttgagccaac tgcccttgcg
     2101 ccacaaaact tcgcagcagg ttttagcggg cctgtttcct acgccgaagc ggttaagcgc
     2161 gcggcgcctg ccgtggtcaa catctatacc cgcaccaaaa ttcaagagcg caggcggcgc
     2221 ctgattgagg acccgctgta ccgccatttt tttaactccg ccgacttagg ccgccaggag
     2281 cgtatgcaat ccgcccttgg cagcggtgtg atcgttagtg aaagcggcta tttgctcact
     2341 aatatccatg taattcgcgg cgccgatgag gtggttgtgc agctgcagga tggccgagaa
     2401 acccaggcta cattagtggg tgtggatgaa gaaaatgacc tggcggtgtt gcatattaag
     2461 gccgataaac tcaccgcaat tcacattggc gcaccgcgca atgcgcaggt gggggacgta
     2521 gtgctggcca tcggcaaccc ctttggtatg gggcaaacgg ttacccaagg cattatttcc
     2581 gccactggcc gccatggttt gggtatcagc accctagaga atttcattca gaccgatgcg
     2641 gccattaacc ccggcaattc aggcggcgcc ttgatcgaca cccagggtaa tttactcggt
     2701 attaacactg ctattttgga tcgtttgggc aattccagcc tggatggcgt tggccttgcc
     2761 gtacctgccg acacggcgct gctgtcgtta aaggatattg tggagatggg taaggtggtg
     2821 cgcggttggt tgggtgttga ggcccagcct gtcacgccct taattgcgca acagctcaac
     2881 ctgcaagcgc tcaacggtgt agtggtgacg gccattttta acaatggccc agcccacaag
     2941 gcaggcctca cccccggcga tattatcctt aaagttaatg ggcaggcggt gggcaacggc
     3001 accctcggca tgcaggaaat attaaaagcg cggccgggcc aggccatggc aatcgaaatc
     3061 tggcgccagg gtgagcttaa aaccctcaac gccgtgctgg gcagcaagcc ggatttaaaa
     3121 gaacaagaag gcgccgcgct gaacgcaaat aaagaataag gcgcccatag gcgccttaaa
     3181 aaatgtgcag gtttttactc gccagagaaa ctagccaatc tgcttccaag ccgattgtaa
     3241 ttcgcctagc aaggtagccg cttcgtccaa taatttaatg tcgtttttgc tatgggcatt
     3301 agtgagaatg cgttgaatat agacgtaaag gttgtccaga ttatgggcca actccccacc
     3361 ggcttcggta tttaaactct cccgcaagcc gcccaccacg ctaattgcac tgctaatttt
     3421 ttgtcccttc agcgctatat cgccaaactg catagcccct tttgcctggg cgatgcgttc
     3481 aatagcgccg tcaaataaca tatcgatgag cttatggggt gaggccacat caacatttga
     3541 gcggtactga accttagcgt acgactttaa tgcagcttgt gtattcacag atggctcctc
     3601 aaacacggta tgtcctcctg ttatcggccc agggggcaaa aactttaggg acacagcaac
     3661 tagggcaccg aagaatcctg ccaaaacagg gttcgatcat tgccacggta ggtgccaaac
     3721 gtataacgcg cgctgggcaa cgcggtgtcg ttgctgtagt tggcgtcgtt attccaatca
     3781 aatcgcaacc aggcataact ggttaaatcc acatccacca aaaacgaacc ggttttaccg
     3841 ctgccgggcg cggaaaaata atgcccagca tcaccggagg aaaaattaac ggcactgcca
     3901 gcaaggctgg tgtaattgcc ggtagtactg cctgccccga tggtgaccga gcggttagcg
     3961 gcaatattaa ttgcgccgaa cggataggtt atggcgctgg tggcgatggt ggtgcaactg
     4021 tcggcagtgt tgcgcgacca tatcgacccc gtccaatagt cggtttggaa attgacattt
     4081 aaattggctg tttccgggcc aaaggcatca tctaatcgca agcggccaaa ataaaaagcg
     4141 ctttggccga ataacaaggc atcgttgctg ccgttggcat cagagtcaaa cgtaatatcg
     4201 gcgcttctta aacttactga atccgtatcg ctaatcgtaa cccccacttt ggtagtcgtg
     4261 taagggccgt cgggcgcact gctgcgcgcc atattcatac tgacacttgc ggccacctgc
     4321 ccgctactcc aagtgccgct taaactcgat aactgcatgc ggctggttaa ttgggtggca
     4381 ctggtggtat cgcgcgcttt taagctgtag cccgccgagg ttaattttac gaatgtgctc
     4441 gtataatttt gtgttgtgct cgcctgctga ttttgcgcag cgagcgttaa attagcggtg
     4501 aaatccttac ctaaataatt aaaagccgtg gatgcgctgc aaaaagacgt catgcttacg
     4561 gacgaagctg caaattgata gggtttaaag cggcctacgt atccagagcg gctcaccata
     4621 ttggtagtag gtgtagtgcc tgcacctaaa taatcaccat cagtaatact cgcggcgatt
     4681 tcaattattc caacttcgcc aaaatacact tgtgtagtgg aacctacgcc actgctaaaa
     4741 ctactgataa cacggccatc actggcagtg gcatcgccat cgcttaaacc tgggtctacg
     4801 ccgccagaag gtaaacgcaa agcggcagca agcgtaattg tttctgcggg ggtttcggca
     4861 ccgaatttag tgagggttgt gttatcggcc aaattggcat tattggcagc gttggtatcg
     4921 gcgtgattat ccggcaggcc atcgctattg gcatcatcgg cggcggaata cgcctttgcc
     4981 gtgacactaa cagtaaaatt agtgcccgcg gtggtaaata cgctaccgga atcagaagat
     5041 gccgccgggt tggatgttac cgcaatataa aatccaaacg gccgcaccac ataagtggat
     5101 gaagacccgg taatttctga actggcgtaa gaattactgc tgtcggcaaa attaagcgca
     5161 tattttccta cgtctgaagt ggaaagagtg aaattagcga tgccgctgga aaagtttagc
     5221 gtgacattat tggagccggg cttggcattg ggtagggcca ccgacgcact actgctatta
     5281 attaaatttg gcgccgtacc ggctgggtcg gaactggcgc gcgtcagcca ggcttttacg
     5341 ccactgacgt tataatcgct agcagcggaa caactggtgc cgccggtatc ttgtttcatc
     5401 atggtcaccc gcaaactgtg ggtgcgcccg gcaataatat cgctggctaa actatccgtg
     5461 ctcgtcacca caaatgcatt gcggctaaat gctagggtgc ttgatgtgct ggttactcct
     5521 gcggagctgt catttaaact aatggttaaa ctctccgcgt gggtgtcggt taaattaatg
     5581 gaaatagtgc ccgcatcaga agtgttaaat acataagtag ccgcaccgga atccgtagcg
     5641 cctgccgtta atgtgccata ggcatcggcg gcggttgcgg tttttgccca atcgccatta
     5701 gcggtggaag tagaaatagt caccgtaccg gcgtaggtgg ttaaggttgc attactagaa
     5761 tcccgcgccg tgatattaat ggttgaagca gcgcagttac tgcccgtacc accgcctaca
     5821 tcaattaaaa aattgtttaa actcgccgcc gaggccgtgc aaaaactgcc gaaggccgcg
     5881 ttgctaattt cagtggattc gtaataaacc gtctgagcgc cgtttaaagt tacattggag
     5941 ccagacaaac cgccgtatac aactgcggtg gaattaagtg ttacggtgct attatgggag
     6001 tataaaaacg cgtgtatagt actggctcca ttcacatcta tactgccatt ggcataaatt
     6061 aataaataac gtgacgatac actcggggtc gtcaccaccc ctcccatcac aacctggcca
     6121 ttcacaaaaa tattgaccgt gcccgtgcta ccgatgcgca attgtttact gccagtgccg
     6181 ttaaaataaa tattgccgtt gatgtaataa ttccccggcg caaaggtaat ggtggtgcta
     6241 tcgccaagtg tccaatcgcc agtgaaataa tagttaccac ccgaggttga aaaagtcagg
     6301 ctgtaggtat tgctgataga taaagaggtg taattccccg ccgcaaaact gggtgtgcca
     6361 ctggtgacat taatagcgcc actggctccg gtgccaccgg aaatagtcat tgattgcgcg
     6421 ggagaagccg cgccgctaat ggtacaggta ataccggtac cacaactgcc ggaacctgca
     6481 ttattggtaa ttaaagggcc agttaatact gaacccgaat tatttactac ggcgccggaa
     6541 tttagcgtaa tgcggcctgt gctcgccgtg tgggattgca aaccatcagg aaataaactc
     6601 gcgcaagtag ccgctaaact acacggcgag cacaacagcc aaaccacaca caccaaaaat
     6661 gaaacaccgg ataatccgcg cattactgca tactcgcttt gacatttaac acacggctgg
     6721 tggccaaatc accgctacca cagctggcgg tagccgtaat taaataaaaa ctgcgcgtat
     6781 tgcccgcatc ggtgctggtg gcgcaatcga aacttaccga acaggctttt aaacctgcag
     6841 cagtaaacga ctgcgaggcc acgtctaacg ccgtgcaatt tgcggtagtt tgcgcccgcg
     6901 tggtggcgcc gttatacagg ttgtataaac ctaattggac acccgactcc gccgcaaaaa
     6961 atgcctgcgc acttaaggcc tcccgcaggg ccatgctatt ggcggaaccc gccatacggg
     7021 cgatagcgat agccagcgcc cctaaggcaa ccacaataaa gagcgccgtg gggattaaaa
     7081 aacccgcctg ttgattcact gttgaatagg ctctagggca cattgcgcac caatacctgg
     7141 tggcgcaata ccaccgtttc gctgcctgcc gttaaagata attgaatgcc gatagaagta
     7201 ttgcgctgct cgctcccggc tgagagggaa aaatttgacg cactttggtt taattgattc
     7261 agcattaaat cggcactgcc gcctgggttt gaatcatcca gtgccgcagt tagaaatcca
     7321 taaccgctgt aacggtacaa actgccgctg agcaaacaaa atcgcttcgg gtttgaagcc
     7381 aaataatagc ggcgcgcagg ggaattacgg gtaaataatt ttgcgccgga gagcgtaata
     7441 ctatttgcac ccaccgcgct gacagtggcg cgggaggaag tgctggcggt ggtataaata
     7501 tctgcggagg ataaaggcgc aacaattaat tgcgtagcgg ccgagccgga atagctcaac
     7561 ggcgcgatca ctacagagct tgaagctgtg ccgccatttt cactatccgc taattgattc
     7621 atataataac caccggccac caccggcacg aactctaaac atttaccggt ggaactaata
     7681 cgcacggaat tgggcacggc ggcacgcaga tagcggctca tttgctcaat agcaacgcgg
     7741 cctttttgca tcactttcac gcggttagtg gtgcgctgat acatatccat ggtatccacc
     7801 aaaaagctac taccaatagc gcccactagc cctaaaatta ccaaaacaaa aattaattcc
     7861 accagcgtaa aaccccgctg ccgccctttc ctctccccaa cagctgcaaa acggcggcta
     7921 gaaattaagt ttgtaggcac ttaacaccac cttagatccg tcactcatta gagttgtaac
     7981 ggtaatgcga cgggcatttg tattggtaac gccgatttca ctgcccgcat ctaccaccgt
     8041 aacactcacc gcaaggcctg tatcgctgct gtcgctgtag ccattgtaat cccccacatc
     8101 atcgtagccg ctatctaccg atattgcggt acaactcgtg cagggcgggt atccgccggt
     8161 tggtgtattt tcagcaaatt tacgggcgag aatttcatct aataaggctt gggttttttc
     8221 taccgcttta acacgcagta ccgggtcaac cgaatgcaag acagcatcgt taaagacctt
     8281 cagcaaacca cctaaaccaa tggcgactac cactaaaaaa aacagcaatt ccaccagcgt
     8341 aaaaccgcgt gtttggcgat aaatcagctg tttgtgtgcg ctgttgcaac ccatagttta
     8401 accagcaaaa tgccaaggct cggcacccat gtgcgcccgt attattattg tgtggatagc
     8461 gttaactgaa aatgtgcgca aacacattca acgcaatacg cgtgataaat acccctgatg
     8521 ccgcacatta aaatccaaca acatctttaa ccgcctgttg aaacgacaaa cacagcgctt
     8581 cgctttaagt gtagcaagca attgggcgga gacgagaaga aaagaaaata aggcaccaag
     8641 gtgctcaggc ggcctattaa aatccacaag cctctgctag cgcgcaaacc ctgtggcact
     8701 tacgttgatt gtaagtgtgc gcgattgctg cgtgagcgta atggtgctgg cagcaacttc
     8761 tccgaggcta ttaaaattga gcgtactggt ggaaacactc acaccttggc tgaggctaat
     8821 aggataggta acggcgccga gcaccacact ttcaccaccg gcaaaaacac cgtcattatt
     8881 agcgtctttg cgtacatcaa gggttgtacc actgagcaaa acacgcactg agcgcgattg
     8941 ggtcatggcc agctgctgcg cggcttgaat acacaatagg gtttgatccc gtgccgcctg
     9001 caaggcataa aggtccggcg acataaattt acccaccgcc gccaccgata ccacacccac
     9061 aatgactaac acagcgatta attcaatcag cgtaaatcct gcgctgttgc aataatcccc
     9121 tctcatgccc atgcgctccg cactatgctt tgtacataat gtagaccata aaaaaggctt
     9181 gggttaaccc caagcctttt tcggtacgtc attacactga gcgccgttaa taccgcagcc
     9241 catagctgcg gtattaaatc aaaatacctg cgctacaaac ctccggccgc accaataatg
     9301 gtaaatgtgg cggttgtact gctgggcccg gttagggtac aggtaacggt tgcaccatcg
     9361 gccactgcgg cagaagcaat gctataaccc gagggcaaag caccattttg cagaagtaga
     9421 gcgccgtctt ggcaattatc cacgttataa aatggatcgg tagtggtatc ggtaagccct
     9481 gcggctaccg caacatcaac agcatggtta agcgctgatg cactttcaat actgccagct
     9541 acacctcgag tggcagccgt agcggcttca ccctgcaaat taacaaaacg cggcaccgca
     9601 gtggctgcca gaatacctag aataacgata acagctatca gctcaatcaa cgtaaagccg
     9661 gattgttgtt tgcccattgt gtgaatctcc acctctaaaa tttaatatag ccactcctaa
     9721 accccctgta agcaatcctt ttccactgat taacagtct
//
LOCUS       DAOQGS010000089         9713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3274_length_9713_cov_13.116898, whole genome shotgun sequence.
ACCESSION   DAOQGS010000089 DAOQGS010000000
VERSION     DAOQGS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3274_length_9713_cov_13.116898"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..2141)
                     /locus_tag="PKZ52_05010"
     CDS             complement(219..2141)
                     /locus_tag="PKZ52_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNG59164.1"
                     /translation="MTCKSLLQALLLASVLGSSTTWAGVLDMPDATELPDAEKKSLLK
                     DMDIPSVKERDPNPEGGPRLNVKEFRIQGLEDDPDFNISKAELIKRVEAIRFDLMGEG
                     EKTKSGYTLKELAEMADLIAQIEEESADQHVGPVEVQKLVFLIREQRRRRGITLGMIE
                     TVADTITQYYRERGFILAKAYIPQQQVRDGVVSLNIMLGKLGSVEVVNAKRINQSLLK
                     RTFNGALDQPVTADNTEENLYLINDIPGVYAQGYFEPGDQVGDTRLKLNVTKEGWFDT
                     SVRIDNHGAKTTGENRVYGEFVLHNPSTLGDEIQLGALRTEEDSSYGAIHYSLPIYTP
                     RLRVKVGASQNDFVSRINFGESSAAGVEYSGSAKVTDADISYHFARGRKLNSSLGLQF
                     TSIKTNIDLSNDKIPVVDTKLDNLSLNYHIDYLHEKRRSLHQFDIGVTSAKETDANRV
                     NTANSGEGELLRDSNAQFFYYNYSLLSFFKNPIGQSDLRLVVNHSLQYAGKALSASNK
                     FDLSGPNRARAFELNHFVADDAAHVAADIIFNTPKWLQFGGPEGSSQNGLTPFLLSDV
                     AYGVTYPEFSTSEKSNALVADLGLGLKLNTEWGLRGNLAWAVQVTDKIKADANEITKD
                     KSTSKNHLYLEFMYSY"
     gene            complement(2306..2743)
                     /locus_tag="PKZ52_05015"
     CDS             complement(2306..2743)
                     /locus_tag="PKZ52_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNG59165.1"
                     /translation="MRATQRYKSLPYNPALKARARALRRAGNLAEVLLWGQLKNKQLL
                     GLDFDRQKIIGNYIVDFYCAEQDLVIEIDGYSHEHTAQQDTRRDKYLTALGLRVVHLL
                     DSDVKQNLEPVMDFLHHYLNAPPSCEPPRPYGAPLQGRGIPSD"
     gene            complement(2967..9692)
                     /locus_tag="PKZ52_05020"
     CDS             complement(2967..9692)
                     /locus_tag="PKZ52_05020"
                     /inference="COORDINATES: protein motif:HMM:NF039490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59166.1"
                     /translation="MELLANGSVASFTGGGQGADAVGDKIIGPVAGAVWQLNADKNTI
                     ADKDGTQLVGNFQQVETLQADSVGEDTLASTLADNTWVLASANSGVLNSTVNFSGFDY
                     LTGSDLKDSFIFNFVGSFGKGVDALKGDNTVQLNLVNRVELDWGDKIVNGVKGANKII
                     GSNSVDSELSLVSAVGLTWTIDRLDGGAISDGSSTFNFDLYKNIKGSKLTDDFRFVNA
                     GRISSSIDGGEGTDSLQGLNRDNAWQLGQTLSITALGDNSAYVTNAIGIERLLGNAAK
                     DTFAVNARVKASISGGEGDDRLVLAAGSQLEGSFNGNGGDNTIESQLSASRWDLSSVV
                     NGVIYNGDEIALRYSSANRLVALPSGDNQLSFANLTADLTWVINGSTNERAAGQVSVV
                     GQSTPASQFSGFNHITGAKGADDLTLAAAIGDINLGEGNNRLTLQTGAKATQVSAGAG
                     SDELRSFAAANLWQMLSATEGKLTTASAEVVSLFTGFEVLTGSGNDRLSGAAAPNKWL
                     LNGPGSGTLNTTLTFTGFGRLQGNGGNDTFEVDAAGGMASIDGGDGDDSLTARNSANT
                     WSFSRNDSGSLRATGEQANYLGFSGIETFTGAVAAADSISLDPQVSQVKLAAGGQAGD
                     ELIVNYRDSLNLSSDGTSWLGVSGVKKVTAQPLSPTRSAQLTSTASLASRWVINEDNG
                     GDWTVGVGSTSALTTHFSGFDKLQGSATADEFILSNGRISTLIDGGTALPGAPRNDSL
                     AAENKYTYWTVTGTGAGSLTYFAANNPPVQQFTGIETLIGNGANDEFRLLADGISINI
                     RGGNSTGFDKVIFNGALDITLGQQDVVGVGIAEIEAFEADQTKQNTLRSSSAGGTWTL
                     SGENSGVLVSGSERLSFSNFQNLLGGKGADLFTLDETARVTGQLSGGEGDNTLRLSHN
                     RDLTASLSADGAGDLAGAFAIARFDHINTLAVTALAPNLSATLTAANQPNQWVVTDNA
                     VVLNGLNYTGFTQLNGQEGVDDFVFNGKQTLSIDGKNGSDSLTLNSAQGTLVNWQLTG
                     AAGAVTAGNAGNPVLIAPLFANLERLIGGAGDDAFVLNATGNRYSFFDGGAGNNRLAS
                     SAANNQWQLGALTSDASPVIDHLNAAELLRIQTLQGSGSDTLLGSDDNNLWQLARGGQ
                     VSLAQSTATGKRSLEASGMGRVQGGKGQDDFVLQETGSGSFARMDGGEVGADSVTGVN
                     TSSVRDSLDARAVSGDVQVNLNEVPASSSLAANQLVVAGIETLQLNSATNNTLYGPTL
                     PNKQAISWQITQAKQGSFAPKQGAHGENTVTFSGVSNLIAGAGEDRFALATADGLGGD
                     LDGGDGTDYLDYSQASLAASQSSFDVVIGDNTRLKNIEGVIGKNTSTMRLVVKGNGSQ
                     WLLDDIDGDATTSDGNNDGRVTLANGKSVDFVDFAALVGTQGVDEFKINGGFNGSITT
                     GEGADSLLVSVNPARAQSIKLINDASDSLSLSWQGAAPSGVKETYKQALSGRESISLE
                     SLNPALGLQPFAIDFSALASINDRLAVDEMRVQTAFESAGFNRLSLTNNALQLAIDST
                     DTANPSAAKIGTWFNVENKTNLVLSGQAGDQLSLSGNSQLAGILSIEKFAISAQGEQS
                     LGAGQINFNSVGSIGSEQKAVRIATPVVQFNNLRGDAWVTSSGDIKLASLDGQGKLNL
                     RADGSIGQTQALSYTRDLTLAASGAINLDSADNRLTGGLSFTSANAINLANGLTNLSA
                     LSAEQFTYSGQDLTASGPMVVAGTASLTSTGSIEALNAANRIARLQVNAGGSAKLALG
                     LDATLVGAKVGGALQAQANGLEVAGDLAAASIDLNAGVPATGAGQLKVNGQLSAPQIA
                     LSAGDIALKTQLQGTDLSLTAKSVSLEKTLDLQGGFTATVDSLQQAAAAAITTKGAFS
                     LAASGALQLDGAISSGRDIDITSGGNLVQSGAWRTEGLAAGGDTKAASVNVVVKSGGS
                     ITQQASASTQSAGNISYEAGGTIKLTSLNAKGEVLVNATAGIEDVNGTATNITGSTAR
                     LISGGNINGLDLSVDSLVGQFAGSAKTVSLRNDKTLRIDRLVTNTSEAGAVTTISVSK
                     GDVIFNNDASKNPEVASLYAKPDLAKEKTGVVNGNFSTGTLNILAEKGRVKATGSVSS
                     IQPDLVANTVNIFSALGISTPGRPLVIYGSTVLYTGTGLNWKPYNAFGNRFEFTSDNA
                     LDLSQLLLAAGDQLIEVEPLEDVNPAVFTDVRNYSADNLSIMLPADQRYEE"
BASE COUNT         1778 a         2754 c         2883 g         2298 t
ORIGIN      
        1 acatactcat taaagttgcc gtgcacgcgg atggcccgct gcaataactc ctgcttgccg
       61 tccccaaggg ccgccttgaa tttaccgcgc aggtaaatgg tgcgaaaggt taacaccata
      121 tacagcagcg caagtacacc ggcataaata cccactatag ccatacaacc tcctcaatta
      181 attgccggat tttaccgcca tggcttttgc ccggcgcgtt agtagctata cataaattcc
      241 aggtacagat gatttttact ggtgctttta tccttggtga tttcattggc atccgctttt
      301 attttatcgg taacttgcac cgcccaggcc aggttaccgc gcaggcccca ctcggtattg
      361 agttttaagc ccaatcccaa atccgcaact agcgcgttac ttttttccga ggtgctaaat
      421 tctgggtagg ttaccccata ggccacatcc gacagtaaaa aaggcgtgag gccattttgg
      481 ctggagcctt ccgggccgcc aaattgcagc catttgggtg tgttaaaaat aatatcggcg
      541 gccacatggg cggcatcgtc ggcgacaaaa tggttcagct caaaggcgcg ggcgcggttg
      601 gggccggaga ggtcgaattt gttgctggcg gacagagctt taccggcgta ttgcaggctg
      661 tggttaacca ctaaacgcaa atccgactgg ccgatggggt ttttgaaaaa gctgagtagg
      721 gaatagttgt aataaaaaaa ctgcgcgttt gaatcccgca acaattcacc ttcgcccgaa
      781 ttagcggtgt taaccctatt ggcatcggtt tcttttgcag aggtcacgcc tatatcgaat
      841 tgatgtaggc tgcggcgttt ttcatggaga taatcaatat ggtaattcag ggagagatta
      901 tcgagtttgg tatccacaac cggaatttta tcattactta gatctatatt ggttttaatg
      961 gaggtaaatt gcaggccgag ggaactgttg agtttgcggc cacgggcaaa atgatagcta
     1021 atatccgcgt cggtcacctt ggcggagcct gaatattcca cacccgctgc ggaggattcg
     1081 ccaaagttaa tgcgcgagac aaaatcgttt tgcgaagcgc ccacttttac tcgcaggcgc
     1141 ggggtgtaaa tgggcaggct gtagtgaatg gcgccgtagc tgctgtcttc ttccgtgcgc
     1201 agggcgccca gctgaatttc atcgccgagg gtggagggat tgtgcagcac aaattcgccg
     1261 tatacgcggt tttccccggt ggttttcgcg ccgtggttgt cgatgcgcac gctggtgtca
     1321 aaccagccct ctttagtcac gttgagtttt aggcgcgtat cccccacttg atcgccgggt
     1381 tcaaaataac cctgcgcgta cacaccggga atatcgttaa ttaaatataa attttcttca
     1441 gtgttgtcgg cggtgaccgg ttgatccagg gcgccgttaa aggtgcgctt gagtaaactt
     1501 tggttaatgc gtttggcgtt gaccacttct accgagccta atttgcccag cataatattc
     1561 agcgacacca cgccatcgcg cacctgctgc tggggaatat aagctttggc gagaataaag
     1621 ccgcgctcgc ggtagtattg ggtgatggtg tcggcgacgg tttcgatcat gccgagggta
     1681 ataccccggc ggcggcgctg ctcgcggatt agaaatacca gcttttgcac ttccactgga
     1741 cctacgtgtt ggtcggcgct ctcctcttca atttgcgcga ttaaatcggc catttcggcc
     1801 agctccttaa gggtgtaacc ggatttggtt ttttcccctt cgcccattaa atcaaaccga
     1861 atagcctcca cccgtttaat taactcggct ttggaaatat taaagtccgg gtcgtcttct
     1921 aaaccctgaa tgcgaaattc tttgacgttt aaacgcgggc cgccctcggg gttggggtcg
     1981 cgctctttaa cgctggggat gtccatatct tttaataggg attttttctc ggcgtccggc
     2041 agttcggtgg catccggcat gtccagcaca ccggcccagg tggtgctgct gcccagtaca
     2101 cttgccagga ggagcgcttg cagaagagat ttacaggtca tggaaattcg ctagttaggt
     2161 tgtacggttg gaaagcggcg caaccacccc gccccttgcg gggcacccct ccgggggagg
     2221 ggaatttagg gcgtgctcat tgtgtgttag ctgcctcgtc tcctttaggg cttaccccta
     2281 gaggccgggc gtgttgtgca cggcgctagt cgctgggtat tcccctcccc tggaggggtg
     2341 ccccgtaggg gcggggtggt tcgcagctgg gcggtgcatt gaggtaatgg tgtaaaaagt
     2401 ccatcacggg ctccaaattc tgtttaacgt cactatcgag taggtgtacc acccttaacc
     2461 ccaaggcggt taggtactta tcccgccggg tgtcttgctg tgccgtgtgt tcgtggctgt
     2521 agccatcaat ttcgattacc aggtcttgtt ctgcgcagta aaagtccact atgtaattac
     2581 cgatgatctt ttggcggtcg aaatctaggc ctaatagctg tttgttcttg agctgccccc
     2641 acagcagaac ctccgctaag ttgcccgctc ggcgcagggc gcgggcgcgc gccttgaggg
     2701 ccgggttgta gggcagagac ttgtaacgct gggttgctcg catgggtacg gctccttgtt
     2761 ggcaatgcca ccggttcacc accgtgcccc ttgcggggca cccctccagg ggaggggaat
     2821 ttgggaggcg gtgcttgcgg ttgttcttaa accaccccgc cccttacggg gcacccctcc
     2881 agtggagggg aatttagggc gtgctgcttt cgatttctct ttaaaccacc ccgccccctc
     2941 cggaggaggg gaatttaagg tgggggttac tcttcgtagc gctggtcggc gggcagcatg
     3001 atggagaggt tgtccgccga gtagttgcgc acgtcggtaa ataccgccgg gtttacgtct
     3061 tctaagggtt ccacttcgat cagctgatcc cccgccgcga gcaataactg ggagaggtcc
     3121 agggcgttgt cgctggtgaa ttcaaagcgg ttaccgaagg cgttgtaggg tttccagttc
     3181 aaacctgtgc cggtgtacag cacggtgctg ccgtagatca ccagagggcg gcccggggtg
     3241 ctgataccca gcgcggagaa gatgttcacc gtattggcca ccaggtcggg ttgaatggag
     3301 ctgaccgagc cggtggcttt cacgcggccc ttttccgcca ggatattgag tgtgccggtg
     3361 ctgaagttgc cgttcaccac accggttttc tctttggcta ggtccggctt ggcgtagagg
     3421 ctggccactt cggggttttt gctggcgtcg ttgttaaaga tcacatcgcc cttgctgacg
     3481 gaaatggtgg tcactgctcc tgcttcgctg gtattagtga ccaagcggtc gatgcgcagg
     3541 gttttatcat tgcgcaggct gacggttttg gcgctgcctg cgaactggcc gaccaggcta
     3601 tccacggaca gatctaagcc attgatattg ccaccgctaa tgaggcgggc ggtcgaaccg
     3661 gtgatgttgg tggcggtgcc gttgacgtct tctatgccag cggtggcatt gaccagcact
     3721 tcacccttgg cattcagact ggtcaacttg atagtgccgc ccgcctcata gcttatgttg
     3781 ccggcgcttt gggtgctggc gctggcctgc tgggtaatgc tgccgccgga tttcaccacc
     3841 acattcaccg aggcggcctt ggtatcgccc cccgcagcca ggccttccgt gcgccaggcg
     3901 ccgctttgca ccaggttgcc gccgctggtg atgtctatgt cccggccgct gctgatggcg
     3961 ccatccaact gcagggcgcc gctggcggct aggctaaagg cccctttggt ggtaatggcc
     4021 gccgccgcag cctgctgcag gctatctaca gtggcggtga agccgccctg caggtcgagg
     4081 gttttctcaa ggctcaccga tttggcggta aggctcaggt cagtgccttg cagctgggtt
     4141 ttcagcgcta tgtcgcctgc gctgagggca atttgcggtg cgctcagctg gccgttgacc
     4201 ttgagctgcc cggcacctgt agcgggcaca ccggcgttga ggtcgatgga ggcagcagcc
     4261 agatcaccgg ccacttcaag cccgttggcc tgtgcctgca aagcgccgcc tacttttgcg
     4321 cccactaagg tggcatcaag gcccagcgcg agtttggcgg agccgccagc gttcacctgc
     4381 aggcgggcga tgcggttggc ggcgttcagc gcctcgatgc tgccggtact ggtaagcgag
     4441 gcagtacctg ccaccaccat ggggccgctc gcggttaaat cctggccgct gtaggtgaac
     4501 tgctcggcgc tgagggcgct gaggttggta aggccgttgg ccaggttaat agcgttggcg
     4561 gaggtgaagg acaggccacc ggttaggcgg ttatcggcgc tgtccaggtt aatggcgccg
     4621 ctggccgcca atgtgaggtc gcgagtgtag ctcagggcct gggtctggcc gatgctgcca
     4681 tcggcgcgca gattgagctt gccctgtcca tcgaggctgg caagtttaat atcgccgcta
     4741 ctggttaccc aggcatcgcc ccgcagatta ttaaactgca ccacaggggt ggcaatccgc
     4801 acggcttttt gctcactgcc gatgctgccg acactgttga aattaatctg cccggccccc
     4861 aggctctgct cgccctgggc gcttatagcg aatttttcga tgctgagtat gcctgccaat
     4921 tggctgttgc cgctgaggct gagctgatcc ccggcttggc cgcttaacac caggttggtt
     4981 ttgttctcca cgttaaacca ggtgccgatt ttagctgcgc tggggttggc tgtatcggtg
     5041 ctatcaatgg ccagttgcag ggcgttgttg gtgaggctta agcggttgaa gcctgcgctt
     5101 tcaaaagcgg tttgtacgcg catttcatcc accgccaggc ggtcgttgat gctggccagg
     5161 gcgctaaagt cgatggcaaa gggctgcagg ccgagggccg ggttaaggct ttctaggctg
     5221 atggactcgc ggccggacag cgcttgtttg taggtctcct tcacaccgct gggcgccgcg
     5281 ccctgccagc tgaggcttag gctatcgctg gcgtcgttga tgagcttgat ggactgggcg
     5341 cgggcggggt tgacgctcac tagtagggaa tccgcgccct cgccggtggt gatgctgccg
     5401 ttaaagccgc cgttgatctt aaattcgtcc accccttggg tgcccaccag ggctgcaaaa
     5461 tccacaaagt ccacgctctt gccgttggcg agcgtcaccc ggccatcgtt gttgccatcg
     5521 ctggtggtgg catcgccgtc gatgtcatcc agcagccact gggagccgtt gcccttcact
     5581 accaggcgca tggtgctggt gtttttgccg atcacgcctt cgatgttttt caggcgggtg
     5641 ttatcgccga ttaccacatc aaagctgctt tggctggccg ccaggctcgc ctggctgtaa
     5701 tccagatagt cggtgccgtc gccgccgtcc aggtcgccgc cgaggccatc ggcggtggcc
     5761 agggcaaaac gatcttcccc ggcgcctgca atcaggttgc tcacgccgct aaaggtcacg
     5821 gtgttttcgc cgtgggcgcc ttgtttgggc gcgaagctgc cctgtttggc ctgggtaatc
     5881 tgccagctga tagcctgttt gttgggcaga gtggggccgt agagggtgtt gttggtggcg
     5941 ctgttaagct gcagggtttc aataccggcc accaccagtt ggttggcggc caggctgctg
     6001 ctggcgggca cttcattgag gttgacctgc acatcgccgc tgacggcgcg ggcatccagg
     6061 ctgtcgcgca cgctgctggt gttcacgccg gtgacactgt ccgcgcccac ttcgccgccg
     6121 tccatacgcg caaagctgcc gctgccggtt tcctgcagca caaaatcatc ctgccctttg
     6181 ccgccttgca cacggcccat gccgctcgct tccaggctgc gcttgccggt ggcggtggac
     6241 tgcgccagcg acacctggcc gccgcgcgcc aactgccaga ggttgttgtc gtcgctgccc
     6301 agcagggtat cgctaccgga gccttgcagg gtttggatgc gcagcagctc cgccgcgttg
     6361 aggtggtcaa tgacggggct ggcgtcactg gttaaggcgc ccagttgcca ctgattattc
     6421 gccgcactgc tggccaggcg gttgttgccc gcgccgccat cgaagaagct gtagcggttg
     6481 ccggtggcat tgagcacaaa ggcatcgtct ccagcgccgc cgatcagtcg ctccagattg
     6541 gcaaacagcg gtgcaatcag caccgggttg ccggcgttgc ctgcggtcac cgcgcctgcg
     6601 gcgccggtaa gctgccaatt caccaaggtg ccctgggcgg agttgagggt gagggagtcg
     6661 ctgccgtttt tgccgtcgat gctgagggtt tgtttgccgt tgaagacaaa atcatccacg
     6721 ccctcctggc cgttcaactg ggtgaaacct gtgtaattaa ggccgttgag cacaaccgcg
     6781 ttgtcggtga ccacccattg gttgggctgg ttggccgctg tgagcgtggc acttaggttg
     6841 ggcgccaggg ccgtcaccgc cagggtgttg atatgatcaa agcgggcgat ggcaaaggcg
     6901 ccggccaaat ccccggcgcc gtcggcgctg aggctggcgg taaggtcgcg gttgtgggac
     6961 aggcgcaggg tgttgtcgcc ttcaccgccg gagagctggc ccgtcacgcg ggcggtttca
     7021 tccagggtga ataggtcggc gcctttaccg cccagcaggt tttggaagtt ggagaagctc
     7081 aaacgctcgc tgccggacac cagtacgcct gagttttcgc cacttagggt ccaggtgccg
     7141 cctgcagagg aggaccgcaa ggtgttttgt ttggtttggt cggcttcaaa ggcttcaatt
     7201 tcagcgatgc ccacacctac cacatcctgc tggccgaggg tgatatccag ggcgccgttg
     7261 aagatcacct tgtcaaaccc ggtgctattg ccgccgcgaa tattaatgct gatgccatcg
     7321 gccagcaggc ggaattcatc gttggcgccg ttgccgataa gggtctcaat accggtaaat
     7381 tgctgcaccg gtgggttgtt ggcggcaaaa taggtcagcg atcccgcccc cgtgccggtc
     7441 accgtccaat aggtgtattt gttctccgcc gccaagctgt cgttgcgcgg ggcccccggc
     7501 agcgccgtgc cgccgtcaat cagggtgcta atgcggccat tgctgaggat aaattcatcc
     7561 gccgtggcac tgccctgcag tttatcgaag ccgctgaagt gggtggtgag agcgcttgtg
     7621 ctgcccacgc ccaccgtcca atcgccgccg ttgtcttcgt tgataaccca gcggctagcc
     7681 aggctagccg tgctggtgag ctgcgcactg cgagtggggc ttaagggctg ggcggtaact
     7741 tttttcacgc cgctgacgcc gagccagctg gtgccatcgc tgctgaggtt gaggctgtcg
     7801 cggtaattga cgatcaactc atccccggct tggccaccgg ccgccaattt cacttggctc
     7861 acctgcgggt ctaggctgat gctatcggcc gccgctacag caccggtaaa ggtttcaatc
     7921 ccgctaaaac ctaagtagtt ggcctgctca ccggtggcgc gcaggctgcc gctatcattg
     7981 cggctaaagc tccaggtgtt ggcgctgttg cgggcggtga ggctgtcgtc gccgtcgccg
     8041 ccgtcaatgc tcgccatacc gcctgcggca tccacctcga aggtgtcgtt gccgccattg
     8101 ccctgcaggc ggccaaagcc tgtgaaagta agcgtggtat tgagggtgcc gctgccgggg
     8161 ccgttcaaca gccatttgtt cggtgccgcc gcgccgctta agcggtcatt gccggagccg
     8221 gtcaacacct caaacccggt gaataggctc accacctcag cgcttgcagt agttaacttg
     8281 ccctcggtgg cactgagcat ttgccacaga ttggcggcgg caaaacttct caactcatcg
     8341 ctacccgccc ctgcgctgac ctgtgtggcc ttggcgccgg tttgcagcgt gaggcggtta
     8401 ttgccctcgc ctaaattaat atcgccaatg gccgcggcga gggttagatc gtctgccccc
     8461 ttggcgccgg tgatatggtt aaagccgcta aattggctgg cgggggtgct ttggcccaca
     8521 accgacacct ggcctgcggc gcgctcatta gtgctaccgt tgatcaccca ggttaaatcc
     8581 gctgtcaggt tggcaaaact taattggtta tccccactgg gcagggccac cagcctgttg
     8641 gcgctgctgt agcgcagggc gatttcatcg ccgttataaa taacgccgtt caccaccgag
     8701 gataaatccc agcggctggc gctcagctgg ctctcgatgg tgttatcgcc accgttgccg
     8761 ttaaaactgc cctctaattg gctgcccgcc gccagcacca aacggtcatc cccctcaccg
     8821 ccgctaatgc tggcttttac ccgcgcattc acggcgaagg tatccttcgc tgcgttgccc
     8881 aataaacgct caatgccaat tgcgttggtg acataggcgc tgttatcccc cagggccgtg
     8941 atgctgaggg tttggcccag ctgccaggcg ttatcgcgat taagcccctg caggctgtcg
     9001 gttccctcac cgccgtcaat acttgagctg atgcgcccgg cgttaacaaa ccggaagtca
     9061 tcggtaagct ttgagccctt aatattttta tacagatcaa aattgaatgt gctgctgcca
     9121 tcgctgatgg cgccgccgtc taagcgatca atcgtccagg ttaaacccac cgcgctcacc
     9181 aggcttaatt cgctatcgac gctgttagag ccaataattt tattggcgcc ttttacgcca
     9241 ttaacaattt tatcgcccca atccagctct actcggttga ctaaattaag ctgcaccgtg
     9301 ttgtcaccct taagtgcatc cacgcctttg ccaaaactgc cgacaaaatt aaagataaaa
     9361 ctgtctttta aatccgagcc ggttaaataa tcaaacccgc tgaaattaac agtactgttc
     9421 aacacgccgc tattagcaga agcaagcacc caggtattat ccgccagggt ggacgcgagg
     9481 gtgtcttcgc caacactatc cgcctgcaga gtttccactt gctggaaatt acccactaac
     9541 tgggtaccat ctttatcagc aatggtattt ttatcggcat ttaattgcca aacagcaccg
     9601 gccaccgggc cgataatttt atcccccaca gcatccgcgc cctggccgcc accggtaaaa
     9661 ctggccaccg aaccattggc taataattcc aaagtatcgc tgccgccacc ggc
//
LOCUS       DAOQGS010000090         9707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3279_length_9707_cov_3.781807, whole genome shotgun sequence.
ACCESSION   DAOQGS010000090 DAOQGS010000000
VERSION     DAOQGS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3279_length_9707_cov_3.781807"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1402
                     /locus_tag="PKZ52_05025"
     CDS             209..1402
                     /locus_tag="PKZ52_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG59167.1"
                     /translation="MKNTQGLKATLAKTSSRLVESSYVLGRLPGLCPWQTVISKELSG
                     ISLFNFKHLGCAPGSTYCPCDTMGTPRGSPGAVMKIRDLDLTDTPINQEHTALGLPPI
                     ERVPLHPVIRAAVVFAVFTLVGLLVLGAAQLFGTGWQPGVDTIAATFKDSLFWNALLV
                     GLLAQIVDGALGMAYGLTSTSFLLATGASPALATASVHMAEVFTTGVSGISHAKFGNV
                     DKKLFLRLLIPGIIGGILGALLVTQIDGKTLKPFITVYLLLMGIYVLSKAFRKQIAAR
                     QSEPKHVAKLALLGGFVDTSGGGGWGPVVTTTLVGTGHDPRTTIGSVNFAEFFLTLTS
                     AASLFVLVDETIWKVVAGLIIGGLFAAPFAAYACKKFSTRTLLILVGLLISGISLFNL
                     YKALA"
     gene            complement(1457..2416)
                     /locus_tag="PKZ52_05030"
     CDS             complement(1457..2416)
                     /locus_tag="PKZ52_05030"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNG59168.1"
                     /translation="MNKQLLLGVGLGVIIATAGTALALRDKAPDFAEVLEVTPIKTTI
                     EPEYAQVVSVTPKRDPNEPEFADVLNVKPLKERGSSQEVCRDQVVTHRRPVQDDNRVL
                     GTATGAIVGGLLGHQVGGGRGKDAATAVGAIAGGVAGNRIQGNMQRNDTYQTTERRCS
                     TQRGQDKVVGYEVTYSFDGVEDVVEMDHKPGRTIPVVNGELVLDQRDTGRYKVKSSKP
                     SQFEVVYSYKGREDMIVLDRDIPVGTRVPVDNGLVDFKAQESGRVLVPREEIRGYDVV
                     YRPDGEKNASVIRLPHQVTVGSRLPMKDGKIQVAVEDTMNRAL"
     gene            2823..3764
                     /locus_tag="PKZ52_05035"
     CDS             2823..3764
                     /locus_tag="PKZ52_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNG59169.1"
                     /translation="MNAPATDLSAQTTHVDAFCAAYRQRQDALSLLKQLNQLAKAVQR
                     HRGMSMALLAGNTVFQGDFILLQKQFERRLAALEAFAELTGGLFTARDKENLHNAWAT
                     IRVDWQQDEVIDNFELHSHFIEQLHGMMVSIGKAVERPVSMLVAEAENSPLPAEDAQA
                     YPRLFKQIELTHFVCAQVPAMIEQIARIRGLATYAAAKGVCDYHHDRKLRYVISCTRA
                     QHEKSRHQAERLETLLAGGIPSLPLIKTYELKLMFLMSTVEKDILSGGTIATTSHQVF
                     KLASEIIDVYVKVVEDGLSLLSRWQEEDLEAWLQMVI"
     gene            complement(3978..4877)
                     /locus_tag="PKZ52_05040"
     CDS             complement(3978..4877)
                     /locus_tag="PKZ52_05040"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNG59170.1"
                     /translation="MTSPQILPIRSPFRVLALLLLTCLLLSGCTVKLAYHFLDWGLQY
                     KLNHYLSLSNDQSQQAKHAIKEFHYWHQRHELPSYVEFLKDARNRLAGPPLTPEEISA
                     YRERLKKFGEQSLDQLLPSISSLLKTLDDKQKQQLFKTLDEDQKDYQDLYLKPPMEDI
                     RTALKNDALKSIKKYMGRLSDAQKARISAWSDAVLPFGAAASAEQDKWEVLMRQLLDA
                     PSRSDYSAQLKNLMLYDFSAWESAHRQIMEHNQTISYELMADMLNSRSREQNASLLEK
                     LDVYIADCEDLIAQAQEEDNKKN"
     gene            5054..5329
                     /locus_tag="PKZ52_05045"
     CDS             5054..5329
                     /locus_tag="PKZ52_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59171.1"
                     /translation="MINSVMQQGSVGMQQSQARMLTNAQEIASSAKSSPQQAQAADVA
                     PVKAVERAPSVEEALLDLQQNRQLFDASAKVFAAGSQSLGRLLDTSA"
     gene            5339..6151
                     /locus_tag="PKZ52_05050"
     CDS             5339..6151
                     /locus_tag="PKZ52_05050"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HNG59172.1"
                     /translation="MLSLSSSPSLAPQPAAYPQVGQIPVGQESPQQRLQRIRPVEQTP
                     SAVSAGEQKPKPYGAKADQPVDALADQDKRKQARQAADEKVAQAELAQLKQLAARDRE
                     VRAHEQAHKAVAGSYGGAIHYEYQRGPDGNRYAVGGEVSIDTAPESSPEATLAKARVI
                     ARAASAPAEPSDQDRAVAAQAAQMEQQALQQLAQQRLDARSPGASSEDPPETGPVPGA
                     GASREVSDAAKAGADEKNERADKTVGQEQGLPKRGGKYGPNVAASLHFHVQA"
     gene            6367..6855
                     /locus_tag="PKZ52_05055"
     CDS             6367..6855
                     /locus_tag="PKZ52_05055"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNG59173.1"
                     /translation="MNDSIYQAPQVELLDTERPVYKASRLRRLFANLIDTFCMSVIVL
                     PAMYFGGLLELATSASLSWSYTLQSGTLWALVFLLFNGLLLVQRGQTIGKLVLSIKIV
                     MQDHSALTVPTLAIRYGVYFLGNLIPLIGPLFGLINVLFIFGEERRCLHDRAAKTIVV
                     NV"
     gene            7848..>9707
                     /locus_tag="PKZ52_05060"
     CDS             7848..>9707
                     /locus_tag="PKZ52_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNG59174.1"
                     /translation="MFRAAKPSRTVWFWALLAGVFSLVSSAALLWNNHRLGQVRFTQA
                     QSQALTAITQQLGAYDALLAQAAALPAQAALPAQAALPGRALPPGILEATAKPSANGL
                     SWQVAAANVPGSPLVAGANLSAWLSSAPSKTSQNFADLTALKGAQPGLVIALRGCESA
                     LPCARWWLAYIPLATLPGALEPGWQLLQEDTKALKPIWPAALKGVLAKEQVQFFTWRN
                     NRYALHLRPPAWPLWGSVLALLASLGIAALTGALLWAREQVSSHQRQQARSQALEEVC
                     EEAMFSIRPDGKLASWSRGAILLWEYGPREVIGRPLAQLFSGPGLNQALAPLAKGKAV
                     RGVELKAKSAKGQAFPVRLNAVPIHNGAGQVVGAALSVADLRPQHSAQTRLLAQEKML
                     AHSMALLEKNNERCFTLEQARRHLQTVLDAVPFVIGYWDKNQINQVANKAHHGRFGVE
                     AAEIPGMSLQELLSAEDYEQQFGRVQAALAGMPQTYEYTQVNEATGAFSHYLGQYLPD
                     TSKTGVRGFYVIEQDITELVEGNLRLGRALEENDVLLTTINNQLLYSVTDAHGVIQDV
                     NDNYCAALGFARTELLGQTHRLVNSGVHPKEFWQGLWESLLAGKPWRAELCNRT"
BASE COUNT         1977 a         2567 c         2871 g         2292 t
ORIGIN      
        1 attcaatcat aaatagagtc tccgcgtgag gtgctaccct cggttgggtt gaggcgcgct
       61 ggccgcgctg gggcaatggg gccaccagtg cgaatgggca caaaagtgta gtggagagac
      121 tgcaagccta caaaaggtta agtctaacga ggtaaagaca atttaatact gagttaaatg
      181 tgcagatgcc cttggcgccc ttgtactgat gaaaaacacg cagggtttaa aagcgacctt
      241 ggcaaaaaca tcaagccgct tagtggaatc aagctatgta ttagggcgct tgcctggcct
      301 ctgcccctgg caaacggtta taagcaaaga attatccggt atttccctgt ttaattttaa
      361 acacctaggc tgcgcgccag gttcaaccta ttgcccctgc gacaccatgg gcacaccaag
      421 gggctcacct ggagcagtaa tgaaaatccg cgatttagac cttaccgata ccccgataaa
      481 ccaagagcat actgccctgg gcctgccgcc gatcgagcga gtgcctttgc acccagtgat
      541 ccgcgcggcg gtggtatttg cggtttttac tttggttgga ttgttagttc tgggcgcagc
      601 gcaattgttt ggcaccggtt ggcagccggg tgtggatacc atcgcggcca cgtttaagga
      661 ttcgttattt tggaatgctt tattggtagg cctgttggcg caaattgtag atggcgcatt
      721 gggcatggcc tatgggctca cttccaccag ttttttattg gccactggcg cctccccggc
      781 attggctact gcttctgtgc atatggcaga agtgtttacc accggagtat ccggtatttc
      841 ccacgcgaaa tttggcaatg tggataaaaa attatttttg cgtttattaa taccgggcat
      901 tatcggcggg attttaggtg ccctcttggt gacgcaaatc gacggcaaaa cccttaagcc
      961 ttttattacg gtgtatttat tgctgatggg tatttatgtg ttgagtaaag cctttcgcaa
     1021 gcagattgcc gcccgccaga gtgagcctaa acatgtggcc aaactagcct tgttgggcgg
     1081 ttttgtggat acctccggcg gtggcggctg ggggccggtg gtaacaacca ctttggtggg
     1141 cacaggccac gacccgcgca ctaccatagg ctcggtgaat tttgcggagt tttttctcac
     1201 cctgaccagt gccgcatcac tatttgtatt ggttgacgaa accatttgga aggtagtggc
     1261 cgggttaatt atcggcggct tattcgccgc accttttgcc gcttacgcct gtaaaaaatt
     1321 ctcaacccgc accttactta ttttggtggg tttgttaatt agcggtattt ccctattcaa
     1381 tttatataaa gcgctggcgt aaaagttagg cgcaaaaaaa agcccggata aatccgggct
     1441 tttgcttaag gaatgattaa agagcgcggt tcatggtgtc ttcgacggcc acttggattt
     1501 tgccatcttt catgggcaag cggctgccaa cggtaacttg gtggggcagg cgaatcacgc
     1561 tggcgttttt ctcgccgtcg gggcggtata ccacgtcgta gccgcgaatt tcttcacggg
     1621 gtactaacac ccggccagat tcttgagctt taaagtccac caggccgtta tcaaccggta
     1681 cacgggtgcc aacgggaata tcgcgatcca gcacgatcat atcttcgcgg cctttgtagc
     1741 tataaaccac ttcaaattgg ctgggcttgc tgctttttac tttgtagcga ccagtatcgc
     1801 gctgatctaa caccagctca ccattgacca ctggaatggt gcggccgggt ttgtggtcca
     1861 tttctactac gtcttcaaca ccgtcaaagc tgtaggtaac ttcatagcct acaaccttat
     1921 cctgaccgcg ttgggtagag cagcggcgct cggtggtttg ataggtgtcg ttgcgctgca
     1981 tattgccctg gatacggtta cccgctacac cgccagcaat cgcgcctacg gcggtggccg
     2041 catctttacc gcgcccgccg ccgacttgat gacccagcag gccgccgacg atggcgccag
     2101 tggcggtgcc taatacgcgg ttatcatcct gtacagggcg gcggtgggtg accacttgat
     2161 cgcggcagac ttcctgggat gatccgcgct ctttaagggg tttaacattt aaaacatcgg
     2221 cgaactctgg ttcgttgggg tcgcgtttcg gggttacgct aaccacttga gcatactccg
     2281 gttcaatggt ggttttgatc ggagtcactt ctaatacttc agcaaagtcc ggggccttat
     2341 cgcgcagagc cagtgctgta ccggcggtgg cgataatcac tcccaaacct acacccagta
     2401 aaagttgctt gttcataatt tgtttcctca aggaaggtta actgcgcttt agttgtacca
     2461 catttggtgc gagcggcaag gggcgatttt gcgtaaatgt tacctgattc tcgccctgag
     2521 gtgatttgtg ggtaacagct ggtgtaactt ttggtaactt gtctcatttt tcacggcaaa
     2581 ctgccgtttt tactgcgctg cagcgcagat tctgcacctg ttctggcgtt tttaaccccg
     2641 gcgctcggcc tgccgactgt tacctcggta gcagtttgcc cgtaaagata tttttgggag
     2701 ggacttgtcc ctatggcatg ggggttgctg aaggcttgag gagaaagcac cgcgcgagaa
     2761 aaaagacata aattagcgct tatggttttt tgcctcgcac caccatgaat cagactatgc
     2821 ctatgaatgc cccagctacg gacctttccg cacaaaccac ccatgtggat gccttctgtg
     2881 ctgcctatcg tcagcgccag gatgctcttt cgttacttaa gcagctcaat caactggcca
     2941 aggccgtgca gcgccaccgc ggcatgagca tggctttgtt ggcgggcaat acggtatttc
     3001 agggcgattt tattctgctg caaaaacaat tcgagcggcg cttggctgct ttggaggctt
     3061 ttgccgagtt aaccggcggc ctttttacag cacgggataa agagaattta cacaacgcct
     3121 gggccactat tcgggtggat tggcagcagg atgaggtgat tgataacttc gagctgcact
     3181 cccattttat tgagcagttg cacggcatga tggtgtccat cggcaaagcg gtggagcggc
     3241 cggtatcgat gttggtggct gaagcggaaa attcgccgct accggcggag gatgcccagg
     3301 cctatccgcg tttgttcaaa cagattgaat tgacccattt tgtctgcgcc caggtgcccg
     3361 ccatgatcga gcaaattgcg cgcattcgcg gcctggctac ttacgctgcc gctaaaggcg
     3421 tgtgcgatta tcaccacgac cgcaaattgc gctacgtcat ttcctgcacc cgcgcccaac
     3481 acgaaaaatc ccgccaccag gctgagcgcc tggagacttt gctggcaggt ggaattccct
     3541 ctttacccct tataaaaacc tacgaattaa agctgatgtt tttaatgtcc acggtggaaa
     3601 aagacatact ctccggcggc accatcgcca ccaccagcca ccaggtgttt aaattggcca
     3661 gtgaaattat tgatgtgtat gtaaaggttg tggaggacgg cctgagtttg ttgagccgct
     3721 ggcaggagga ggatttagag gcttggttgc agatggtcat ttaatttctg ccgaataact
     3781 aaggaacctc tgattaagtc tggagcggca gggatatgtg tgttttttca aaacagtcgc
     3841 gaatgtaggc ttggtattta atcagaggtt ttaagaaggc tatacctgtg gggacagcca
     3901 aaattcatcc cgccaggaga ggggggcaac agcaggtgat ggggtggaat gatgtgcgcc
     3961 accggtggcc tgagggttta gttttttttg ttatcttctt cctgcgcctg cgcaattaaa
     4021 tcctcgcagt cggcaatata cacatccaat ttttcaagca aagacgcatt ttgctcgcgg
     4081 ctgcggctgt tgagcatgtc ggccatgagt tcatagctga tggtttgatt gtgttccatg
     4141 atctgtcggt gggcgctctc ccaagcggaa aaatcataca gcatgagatt ttttaattgt
     4201 gctgagtaat cgctgcggct gggggcgtcg agcagctggc gcattaatac ctcccattta
     4261 tcctgctcgg cagatgcggc ggcgccaaaa ggcaatacgg cgtcgctcca ggcgctgatg
     4321 cgggcttttt gggcgtcgct taagcggccc atgtattttt taatggattt taaggcgtcg
     4381 ttttttaagg cggtgcggat atcctccatg ggcggtttta ggtagaggtc ctggtagtcc
     4441 ttttggtctt catcgagggt tttaaatagt tgttgttttt gtttgtcatc cagggttttt
     4501 aataagctac tgatgctcgg cagtaattgg tctagggatt gttcgccaaa tttttttaag
     4561 cgctcgcggt acgcgcttat ttcttccggg gttaacggcg ggcctgccag gcggttgcgg
     4621 gcgtctttta agaattccac gtagctgggc aattcatggc gttgatgcca gtagtgaaat
     4681 tctttgatgg cgtgtttggc ctgttgggat tggtcgttac tgagggataa ataatgattt
     4741 aacttgtatt gcaggcccca atccaaaaaa tggtaggcca gttttaccgt gcaaccgctc
     4801 aacaataggc aggttaataa cagcagtgct aatacccgaa acggggagcg aataggaaga
     4861 atttggggtg aggtcattaa tcgggtggcc gggttgaagg taggtgaaag caagcaaata
     4921 gccgctgcca aggattattg tgtttttaat aaaaattccg tcttaaagtt ttgcccaatt
     4981 ctgccgccgg gggtgcaagt ccatactaac cttaatgcat gagccagaga tttaagcgcg
     5041 ggttgaggtg ggtatgatta attcagtaat gcagcagggc agtgtgggca tgcagcaaag
     5101 ccaggcgcgc atgctaacaa acgcccaaga gattgccagc agtgctaaga gttcaccgca
     5161 gcaagctcag gcggcggatg tggcgccagt gaaagcggtg gagcgggcgc cctcggtgga
     5221 ggaggcatta ttggatttac agcagaaccg ccaattgttt gatgcctcgg ccaaggtctt
     5281 cgccgccggt agccaatcct tggggcggct gctggatacc tctgcttaag gattttttat
     5341 gttgagcctg tcatcatcgc cgtcccttgc gccgcaaccg gccgcctacc cgcaagtagg
     5401 gcagattccg gtggggcagg agtcgcccca gcagcgcttg cagcgtattc gcccggtgga
     5461 gcaaaccccc tctgcggtgt cggctggcga gcaaaagcca aaaccctacg gcgccaaggc
     5521 ggatcagccg gtggatgctc tagcggatca ggataaacgc aagcaggccc gccaagcggc
     5581 ggatgagaag gtggcgcagg ctgagcttgc gcagttgaaa cagctggcgg ctcgcgaccg
     5641 cgaagtgcgc gcccatgagc aggcccataa agcggtggcg ggcagttacg gcggcgctat
     5701 tcactatgaa tatcagcgcg ggccagatgg caaccgctat gcggtgggcg gtgaggtgtc
     5761 gattgatacc gcgccggagt cttcaccgga ggccacgctt gccaaggcgc gggtgattgc
     5821 gcgggcggcc agcgccccgg cggagccctc ggatcaagac cgtgcagtgg cggcccaagc
     5881 cgcgcaaatg gagcaacagg cgctgcagca attggcccag cagcggctgg atgcccgcag
     5941 ccccggcgcc tcatcagagg acccgcctga aaccgggcct gtgcctggag cgggcgctag
     6001 ccgggaggtt tccgatgccg ctaaagccgg tgctgatgaa aaaaatgagc gcgcggataa
     6061 aactgtcggc caggagcaag gccttcccaa gaggggtggc aaatacgggc caaatgtggc
     6121 ggccagcttg cattttcacg tgcaggctta actcggttag aaggcctggc actgtcggca
     6181 ggcttgactg ggccagtagg ctcagctcgg cagccctcca ccgactttat taaaggtgat
     6241 tatctgccga ggccgcctcg gtatgtgcgt gggcatgcgt atgtaggccg gttgagctac
     6301 agcccttaac tgcgtaggct ggtcgagcgg ctgatggccg cagaccctca cctaccagga
     6361 tcccctatga acgattccat ttaccaagcc ccccaggttg agctgctcga caccgagcgg
     6421 cccgtgtata aggccagccg tttgcggcgg ttgtttgcca atcttatcga tactttttgt
     6481 atgtcggtga tcgtgctgcc ggccatgtat ttcggcggat tgctagagct tgctaccagc
     6541 gcctcgctgt cctggagcta taccctgcaa tcgggcaccc tctgggcgct ggtgtttttg
     6601 ctgtttaacg gtttgctgct ggtgcagcgc gggcagacca tcggcaagct ggtgttgtcg
     6661 atcaaaatag tcatgcagga tcactccgcg ctgacggttc ctaccctggc tattcgctat
     6721 ggggtgtact ttctgggcaa ccttattccc ctgatcggcc ccttatttgg cttgatcaat
     6781 gtgttgttta tcttcggcga agagcgccgc tgcctgcacg accgggcggc caaaactatt
     6841 gtggtgaatg tttagcggtt acagactcac cccaaacatt ccgctcgcgt cttgatctag
     6901 cctggattgc gctgcgctgc atccaggcta ccctcagggg aggcttatgc ttagctgcaa
     6961 ggccctccct ttatcaaaac ctgccggtcg ctgcccggat attccgcgaa gcggttgagt
     7021 acctataccc cctaactaac cccctcagcc cgccgccaaa cctcgccgcc gagtgttttt
     7081 agacgctcgc ggcaggttgg agctgccaat tcctatgttt acacaagggc gctgcagctt
     7141 ttttgtgcgt gcaggcagta aaaacatagc tccgcctatg tttttactgc ggcatccttg
     7201 tatcgcagac ccattagcta tcttttttgc tcggcgagct gagtttcaac tgcctcgggg
     7261 tagtgatgaa gtgggtgggg gtactggcga agtggggggg taggcaatgt cattaaattg
     7321 ggagggggta gtaacccttg tggttgccct gtgttgatca ggatttgcgg ggagtgggca
     7381 cccacagggg gcgcccctac agctgttggc gcgttgggta ggtaaacgcc ccgccgcagg
     7441 gccatgttat tagggtaaga ggtggaatcc gcacggccta aaattctcct cccatgaagg
     7501 gggcggtgtg tctgccgggg tggttgcggt ggcgcggcct gtgcctttcc atgggatgag
     7561 gaaatttttt accgtatagc agccgatcat gaacgataca catcaccacc ccgcccctgc
     7621 ggggcacccc tggcgaggga ggggaatttt aggctctgcc gcagaagttt cagtctgcag
     7681 gcccttatct gcaccacctc gccttccaag cacatgcgcc cggaccaacg ccgccgcccg
     7741 tatgcagatg cgtgtagggc gccgtgggcg aaatgcctgg cttgggtcta tggttaagga
     7801 agctatagac cttattcccg cgctgccgca aggagccaat agtccgtatg ttcagggctg
     7861 ctaaacctag ccgaaccgtt tggttctggg cgttgcttgc tggtgtgttc agcctggtga
     7921 gcagtgccgc tctgctgtgg aataaccacc ggcttgggca ggtgcggttt acccaggccc
     7981 aaagccaagc ccttacggct attacccagc agctgggcgc ctatgatgcg ttgcttgccc
     8041 aggccgccgc gctacctgcc caagctgcgc tgcctgccca agccgcgctg cctgggcgcg
     8101 ccctgccgcc gggtattctg gaggctaccg ccaagccctc tgccaatggc ctcagctggc
     8161 aggtggcggc ggccaatgtg ccgggcagcc cgctggtggc gggggctaat ctatccgcct
     8221 ggctaagttc tgcgcccagc aaaaccagcc aaaactttgc cgatctcacc gccctaaagg
     8281 gcgcccagcc ggggctggtg attgccctgc gcggctgcga gagcgcgctg ccctgcgccc
     8341 gctggtggtt ggcctatatt ccgctggcta ccttgccggg ggccttggag ccgggctggc
     8401 agctgctgca ggaggacacc aaagcgctta aacccatctg gcctgccgcg cttaaaggcg
     8461 tgctggcgaa ggagcaggtg cagtttttta cctggcgtaa caaccgctac gccctgcact
     8521 tgcgcccacc cgcctggccc ctgtggggca gtgtgctggc gctgctggca agcctgggca
     8581 ttgccgcctt aaccggtgcc ctgctgtggg cgcgggagca ggtgagcagc caccagcgcc
     8641 agcaggcgcg cagccaggcc ctggaggagg tgtgtgaaga ggctatgttc agcattcgcc
     8701 cggatggcaa gcttgccagc tggagccgtg gggctatttt gctctgggag tacggccccc
     8761 gcgaggtgat cgggcggcct ttggcgcagc tgttcagcgg ccctgggctc aatcaggccc
     8821 ttgcgcccct ggccaaaggc aaggcggtgc ggggggtgga gttaaaagct aaaagcgcca
     8881 aggggcaggc cttcccggtg cgcttaaacg cggtgcctat tcataacggg gctggccagg
     8941 tggtgggcgc cgccctcagt gtggcggacc tgcgccccca gcattcggcg caaacccgcc
     9001 tgctggccca ggagaaaatg ctcgcccaca gcatggcgct gctggagaaa aacaacgagc
     9061 gctgcttcac cctggagcag gcccgccgcc accttcaaac ggtgctggat gcggtgccgt
     9121 ttgtgatcgg ttattgggat aaaaaccaaa tcaatcaggt ggccaacaag gcccaccacg
     9181 gccgcttcgg ggtggaggcg gcggagattc ccggcatgag cttgcaggaa ttgctcagcg
     9241 ccgaggatta cgagcagcag tttggccgcg tgcaggcggc cctggcgggt atgccgcaaa
     9301 cctatgaata cacccaggtg aacgaggcca ccggggcctt cagccactac ctgggccaat
     9361 acctgccgga caccagcaaa accggcgtgc gcggctttta tgtgatcgag caagacatca
     9421 ccgaattggt ggagggcaac ctgcgcttgg gccgcgccct ggaggaaaac gacgtcctgc
     9481 tcaccaccat caacaaccaa ctgctctact ccgtcaccga tgcccacggg gtgattcaag
     9541 acgttaacga caactactgc gccgcccttg gctttgcgcg cacggagtta ctggggcaaa
     9601 cccaccgttt ggtcaattct ggggtgcacc ccaaggagtt ttggcagggc ctgtgggaga
     9661 gcctattggc gggcaaaccc tggcgggcgg agctgtgcaa ccgcact
//
LOCUS       DAOQGS010000091         9666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3301_length_9666_cov_4.639476, whole genome shotgun sequence.
ACCESSION   DAOQGS010000091 DAOQGS010000000
VERSION     DAOQGS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_9666_cov_4.639476"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1611)
                     /locus_tag="PKZ52_05065"
     CDS             complement(115..1611)
                     /locus_tag="PKZ52_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNG59175.1"
                     /translation="MSIIRLTASDGRPAEFVDQVRASGGMKDVYFSPSEKYVVAFYRN
                     PLDEASRERLAAITGPYRERIFNQTGGDTWRHSFCWPTATVEYQGRTGVVVPFYEANF
                     FFAQGSRNQDMLGIKGREKESKWFTSASNRARFLAPEELGTWLSQLRVSLSLARAVRR
                     LHAAGLAHSDLSYKNVLIDPITGRACIIDLDGLVVPGKFPPEVVGTPDFIAPEVVASQ
                     HLSKGDPARKLPSINTDLHALATLIYMYLLLRHPLRGDNSHHEDPVQDELLAMGEQAL
                     FIEHPSNRANRVNPANLKAYELPWKNPAQTPYTLTGPYLSQLFERAFIDGLHNPQARP
                     GADEWEIALVKTLDLIQPCTNTSCPQAWYVFDNSQSPACPFCHTPYKGQLPVLNLYST
                     WGEGSFRPDNHRLLVYSNQSLFPWHTNRDIFPNEKLTSEQKQRQAYCVLHEGQWWLVN
                     EALPELTLVNSQTPIPIGDKIALADGQQLLFSKQKGGRLAVVQMVKGA"
     gene            complement(1608..2855)
                     /locus_tag="PKZ52_05070"
     CDS             complement(1608..2855)
                     /locus_tag="PKZ52_05070"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNG59176.1"
                     /translation="MASWFKKPGSEHLAAPAPVSSFSLPNGRVGQAYRLELASLIPRF
                     KAALALEAPAASGLALDGSEITGTPLAAGEFSLQLRYLAQGQEQSSLARLQLAVVQDP
                     KNLWKNLPSDSAGLFASADTQVQQQAGLIGASTRGRSHAHVGGYRDDAFAIEALASGW
                     QIVIVADGAGSARYSRRGAELICQTAASSLRAQLTGPIADSLSASSRAWAQEPQSEEL
                     QQRLKTQLAALLGQAAYQALAAIHAQASELADSQVKDFASTALIGLCRHFPTDGRGPG
                     GSLCACYWVGDGAAAVYSQGRGVRLLGLGDSGEFAGQTRFLDASAVSNEVITQRTCFA
                     WAEDLTAFILMSDGVSDAKFPTAASLASLPHWDTLWAELNPLLSGVGAAEALLEWLNF
                     WSPGNHDDRTLVLALPPGQKEPL"
     gene            complement(3252..4295)
                     /locus_tag="PKZ52_05075"
     CDS             complement(3252..4295)
                     /locus_tag="PKZ52_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerY-C metal binding domain-containing protein"
                     /protein_id="HNG59177.1"
                     /translation="MRRLPIFFLLDVSESMVGDNLRQLQVGLERLVKSLRTDPHALET
                     VHLSVLAFAGKTRTLAPLTELAMFYPPRLPIGAGTCLGVALEHLMDELNRNLIKTTPL
                     QKGDWRPVVYLMTDGKPTDDPSGALERWRREFASRVTLVAIAIGKHASLTTLEQLTDN
                     VLLLDASTDEEFRRFVDWVTQSVVAQSRSLSANAVSLAKLDEGILTKIGLDKSAAVVD
                     EDFVVLTGRCQTSKLPYLIKYERLSKQVNSLAFNLRLDNYHLAGVHALEPDYYEMSDN
                     RVMTRTVNTDELVGAPGCPHCGNRIGFAMCSCGQIMCIAGEGPAVCPSCSKTCNFGYD
                     PDGEGFDVTRGRG"
     gene            complement(4310..4960)
                     /locus_tag="PKZ52_05080"
     CDS             complement(4310..4960)
                     /locus_tag="PKZ52_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNG59178.1"
                     /translation="MSTRRLPVYLLLDTSGSMRGEPIASVNVGLRSMLTSLRQNPYAL
                     ESVYLSIITFDSSVQQVLPLTALPQLQLPEIVCPNSGATFLGEALEFVCDRLAQDVVK
                     ASSEQPGDWRPLLFIMTDGKPSDTLAYEEAIPRVQAAGFAAVIACAAGPKSVPDQLKE
                     LTNQVVSLDTMDSASFASFFQWVSAAVVAGGQNDGQTSAGQAGTLPPPPPEINLVF"
     gene            complement(5218..6402)
                     /locus_tag="PKZ52_05085"
     CDS             complement(5218..6402)
                     /locus_tag="PKZ52_05085"
                     /inference="COORDINATES: protein motif:HMM:NF026041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HNG59179.1"
                     /translation="MAAGGPLWSMPLKGVSLRYMHEQANRLTIGLETEPKAVACSACG
                     SKAVVGYGRLVWRIRDLPIENKAVLLEIARRRLRCKDCKVTFNEPVANIAEAHRTTTR
                     LAQRLWQLGLYYPFTALAPKFGLDEKTLRGIFSDRFALALGQLNRQVLSQAPRRVVLH
                     RPLIQRQQRLLWLNADLDTLVDLNPLLTADRLEVSLKRLGLLVPGAHSELWLPPDLAL
                     LHLLKAQLPVPSLVKLRLHPWALRQLCLSLAAQARGDELAAALVALAQAPSAQAARQA
                     LEQLLAAPRPAGPPKDIARALAELASLQALLGEQALAAFDAPALPLDSLLTQLDERLA
                     GSLAKRSFDAVCALLLLDKSLQVSARTGLAREGRRLDYQKAHYGTSLVRLLAKLAQLL
                     PL"
     gene            6671..7705
                     /locus_tag="PKZ52_05090"
     CDS             6671..7705
                     /locus_tag="PKZ52_05090"
                     /inference="COORDINATES: protein motif:HMM:NF010620.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNG59180.1"
                     /translation="MKHFTWSFIITIVLMALAGWWGYSRGGLPGALQALLITSILAVM
                     EVSLSFDNAVVNASVLRTWNAFWQKLFLTVGILIAVFGMRLVFPIAIVAVATGLGVME
                     VMDLALNNPDEYSRQLTDHHPQVAAFGGAFLMLVFLNFLFDEEKDVHWLGWIEEKFGS
                     HGNEGLSTLLTMLAVYGASTLVPEAIKLSVLMSGIGGIAIYLAVNYLSGLLEAKEEVS
                     HTGEVILKGSIGGFLYLEVLDASFSFDGVIGAFAITKDVVIIMIGLAIGAFFVRSMTI
                     FLVRQGTLEAYVYLEHGAHYAIGILAAIMLLSVTYHIPEWFTGLSGVAFIGLSLWSSI
                     RHRRLEAAKA"
     gene            7716..8294
                     /locus_tag="PKZ52_05095"
     CDS             7716..8294
                     /locus_tag="PKZ52_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004235930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNG59181.1"
                     /translation="MAISLRKGRNLNLSKTDPSLRHLRLGLGWNANADKDADFDLDAS
                     VFMLTTEGKVRGDGDFIFYGQLRSSCGGVEHTGDNRTGLGDGDDEMLSIRLAKIPLEI
                     SRLVVAVTIHEAEARGQNFGKVEDAFIRLVNLDTGTELARFDLTEDYSNQTALIFGEL
                     YRAGDDWKFKAVGQGFAGDLRALALLHGVDVQ"
     gene            8396..8971
                     /locus_tag="PKZ52_05100"
     CDS             8396..8971
                     /locus_tag="PKZ52_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNG59182.1"
                     /translation="MAISLQKGGNVNLSKEAPSLKKLIVGLGWDPRATDGAAFDLDGS
                     AFLLKGDGKVRSDADFIFYNNLKSADGSIVHLGDNTTGQGDGDDEQLTIDLGVVPADV
                     ERVVVAVTIHDAEARRQNFGQVGKAYIRVLNADGNKEIARFDLSEDGSTETAMIFGEV
                     YRAGAEWKFKAVGQGFAGGLGPLARSYGINA"
     gene            9056..9301
                     /locus_tag="PKZ52_05105"
     CDS             9056..9301
                     /locus_tag="PKZ52_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNG59183.1"
                     /translation="MLELRPNCECCDKDLPPDSLDAMICTFECTFCRSCATTVLAGTC
                     PNCSGNLVARPVRPAAQLHRYPASSLRVVKPGGCPSP"
     gene            9337..>9666
                     /locus_tag="PKZ52_05110"
     CDS             9337..>9666
                     /locus_tag="PKZ52_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNG59184.1"
                     /translation="MTVFTPTGDVDPFLHVAMQRGDKIYCESGAMVMMDATLDLKGKM
                     GGGFGAALMRTFANGESFFQQHIEAVRGDGDCLLSPAMPGAMDVVEVGKNRYLLNDGA
                     FVAATSGV"
BASE COUNT         1903 a         2840 c         2941 g         1982 t
ORIGIN      
        1 agcgccagca aaataccttg gtttgaggct tttggcgcct ccccctttta aagggggagg
       61 ctgggagggg gttatgggca gggcacccgc attctgcaaa cagaccaaaa acgctcaagc
      121 gcctttcacc atctgcacca ccgccaagcg cccgcccttt tgtttggaga acagcagttg
      181 ctggccgtct gccagggcga ttttgtcgcc gatgggaata ggggtttggc tgtttaccag
      241 ggtgagttcc ggcagggctt cgtttaccag ccaccactgg ccctcgtgca atacacaata
      301 agcctgccgc tgtttttgct cggaggtgag tttttcgttg gggaatatgt ctcggttggt
      361 gtgccagggg aagagggatt ggttggagta gaccaacaag cggtggttgt ccggccggaa
      421 gctgccctcc ccccaggtgg agtagaggtt gagcaccggc agctgccctt tgtacggcgt
      481 gtggcagaag gggcaggcgg ggctttggga gttgtcgaac acataccagg cttgcgggca
      541 gctagtgttg gtgcagggct ggataagatc cagggttttc accagggcga tctcccactc
      601 atcagcgccg gggcgggctt gggggttgtg cagcccgtcg ataaatgccc gttcaaacag
      661 ctggctgagg taggggccag ttagggtata gggggtttgc gccgggtttt tccagggcag
      721 ctcataggct ttgaggttgg cggggttcac ccgattggcg cggttgctgg ggtgttcaat
      781 aaacagcgcc tgctcgccca tggccaagag ctcatcctgc accgggtctt cgtggtggct
      841 gttgtcaccc cgtaatgggt ggcgcaacag cagatacatg tagatgaggg tggctagggc
      901 gtgcaggtcg gtgttgatgg agggcagctt gcgggccggg tcgcccttag atagatgttg
      961 gctggctacg acttccgggg cgataaaatc cggcgtgcct accacctcag gggggaattt
     1021 accgggcact actaggccgt ccaaatcgat aatacaggcg cggcccgtga tggggtcgat
     1081 gagcacgttt ttgtaactta ggtccgagtg ggccaggccc gccgcgtgca ggcggcgcac
     1141 ggcgcgggcg agactcaagc tcaccctcag ttgacttagc caagtgccca gctcttcagg
     1201 ggctaaaaag cgcgcgcggt tgctggcgga ggtaaaccac ttgctctctt tttcgcggcc
     1261 ttttatgccc aacatgtcct ggttgcgcga gccctgggcg aagaaaaaat tcgcctcgta
     1321 aaacggcact accaccccag tgcggccttg gtattccacg gtggcggtgg gccagcaaaa
     1381 gctgtggcgc caggtgtcgc cgccggtttg gttgaaaatg cgctcccggt aagggccagt
     1441 gatggccgcc aggcgctcgc ggctggcctc atccaagggg ttgcggtaaa aggccaccac
     1501 gtatttttcg ctgggagaga aatacacatc cttcataccg ccgctggcgc gaacttgatc
     1561 gacaaattcg gcggggcggc cgtcgctggc ggtgaggcgg ataatgctca taggggctcc
     1621 ttttggcctg ggggtaatgc cagcaccagg gtgcggtcgt cgtggttgcc ggggctccaa
     1681 aaatttaacc attccagcaa cgcctctgca gcgcctacgc cgctgagcag tgggttaagc
     1741 tccgcccaga gggtgtccca gtggggcaag ctggcaaggc tcgctgcagt ggggaatttg
     1801 gcgtcgctga cgccatcgct catcaggata aacgcggtta aatcctccgc ccaggcaaaa
     1861 caggtgcgct gagttatgac ttcgttgctg acggcactgg catctaaaaa ccgggtttga
     1921 ccggcgaatt ccccgctgtc gccaaggccc aacagcctta ccccgcggcc ctggctgtaa
     1981 accgcggcag cgccatcccc cacccaataa caggcgcaca ggctgccgcc ggggcctcgg
     2041 ccatcggtgg ggaagtgacg gcacaggccg atcagggcgg tgctggcgaa gtcttttact
     2101 tgggaatcag ccagttcgct cgcctgggcg tggatagccg ccagggcttg ataggcggcc
     2161 tggcccaaca gggcggccag ttgagtttta agacgctgct ggagttcttc gctttgcggc
     2221 tcttgggccc aggctctgct gctggcgctt aaggagtcag cgatagggcc ggtaagttgc
     2281 gcgcgtaaac tgctcgcggc agtttggcag atcagctcag ccccccgccg ggagtaacgg
     2341 gcggagcctg cgccgtccgc cacaatcaca atctgccagc cgctcgcgag ggcctcaatg
     2401 gcaaaggcgt cgtctcggta accgcccaca tgggcgtggg agcggccccg cgtgctggca
     2461 cctatcagcc ccgcctgctg ctgcacctgg gtatcggcgc tggcaaacag gcctgcactg
     2521 tcgctgggca ggttcttcca aaggtttttc gggtcctgta ccaccgctag ctgcaggcgg
     2581 gccaggctgc tctgctcctg gccctgggct aaatagcgca gctgcaggct gaactccccg
     2641 gctgccaggg gggtgcccgt tatttcgctg ccatcaagcg caaggccaga tgcggcgggg
     2701 gcttccaggg ctagggcggc cttaaagcgg gggataagac tggccagctc cagccggtaa
     2761 gcctggccta cccggccgtt gggcaggcta aaactgctta cgggcgcagg cgccgccagg
     2821 tgttcgctgc cgggcttttt aaaccatgag gccatggggg gctcctgtgg ggcatttaat
     2881 tgagcgagga gataacgaat attttccgca ccgggcggag tctgatctgc ctcttttggg
     2941 cggtgtctcg ggggtaatca ttttttgtag cctggatgga gtgcagcgta atccgaggtg
     3001 gtctgagcat cccggattac gctgcgctcc atccaggcta cccatctaaa ccccctcccg
     3061 gcctccacct taaaaagggg gaggagctaa aaagccttaa accaagtact tttgctcggt
     3121 ttaaggcttt ttgctccctc cccttgtcaa ggggagggct ggggaggggt aaaaaacggg
     3181 ctcaggcatt cgcctagatt tctcccctgc aggtaatgtc aggagtgcac agccaatgcc
     3241 aggctggttt attatccccg cccccgcgtc acatcaaacc cctcaccatc tgggtcataa
     3301 ccaaaattgc aggttttgct gcaactgggg cagacggctg ggccctcccc ggcaatacac
     3361 ataatctgcc cacagctgca catggcaaag ccgatgcggt tgccgcagtg ggggcagccg
     3421 ggggcgccca ccagttcgtc ggtgttcacc gtgcgggtca tcacccggtt gtccgacatc
     3481 tcgtaataat ccggctccag ggcgtgtacc ccggccaaat ggtagttatc caggcgcagg
     3541 ttaaaggcca gggagttaac ctgtttagag agccgctcgt acttgatgag atagggcagc
     3601 ttgctggttt ggcagcggcc ggtgagcacc acaaagtcct cgtccaccac cgccgcgctt
     3661 ttatccaggc caattttggt gagtatgccc tcatcgagtt tggccaggct caccgcattg
     3721 gcagaaaggc tacggctttg ggccaccacc gactgggtca cccagtccac aaagcggcga
     3781 aattcctcgt cggtactggc atccagcagc aacacgttgt cggtcaactg ctcaagagtg
     3841 gtaagggaag cgtgtttgcc gatggcgatg gccaccaggg ttacacggga agcaaattcc
     3901 cgccgccagc gctcgagggc cccgctgggg tcgtcggtgg gtttgccgtc ggtcatcaga
     3961 tacaccaccg gccgccaatc gcccttttgc agcggggtgg ttttgatcag gttgcggttt
     4021 aactcatcca tcaaatgctc aagcgccacc cccaagcagg tgcctgcgcc tatgggcagg
     4081 cggggcgggt aaaacatcgc cagctccgtt aagggcgcca gggtgcgggt tttacccgcg
     4141 aaggccaaca cggaaagatg cacggtctcc agggcgtggg ggtccgtgcg caggcttttc
     4201 accagccgct ccaggcccac ctgcagctgg cgcaggttgt cccccaccat ggattcggac
     4261 acatcgagca gaaaaaatat gggtaaacgg cgcatagcgg gctccttgtt taaaaaacga
     4321 gattaatttc cggcggcggt ggcggcaggg tgcccgcctg cccggctgag gtttggccgt
     4381 cattctggcc accggcgacc acagcggcgc tcacccattg gaagaaagag gcgaagcttg
     4441 cgctatccat ggtgtccagg ctcaccacct gattggtgag ttctttgagt tgatccggca
     4501 cgctttttgg gcctgcggcg caggcgatca ccgccgcaaa acccgccgcc tgcacacggg
     4561 ggatggcctc ttcataggcc agggtatccg agggtttgcc gtcggtcatg ataaataaca
     4621 gggggcgcca atcgccgggc tgctcgctgc tggccttcac cacatcctgc gccaagcggt
     4681 cgcagacaaa ctccagggcc tcgcctaaaa acgtcgcgcc ggagttgggg cagacaattt
     4741 ccggcagttg caactgcggc agggccgtca gcggtagcac ctgttgcacg ctggaatcaa
     4801 aggtaatgat gctcaaatac acgctctcca gggcgtaggg gttttgcctg aggcttgtaa
     4861 gcatactgcg caggcccaca ttgacggatg caatgggctc accgcgcatg gagccggagg
     4921 tgtccagcag cagataaacg ggtagacggc gggtggacat aggcgggctc ctgaagaata
     4981 acaagtgatg gcgctggcag ggcggcgcag ttcaaccgcc atacaaccgg cgccagaaaa
     5041 ccacacttag cggctgcaat tcaaccgcct gggttcacag tccaggcggt ttgcaggcaa
     5101 tgtcatcatt aaatgcactt gccctgagcc tgctgagggc ttggatcagg atttgcgctg
     5161 gagcgggacc cgcaaggggt gcccctacgg cggttcctta gcctaataac agtgggttta
     5221 cagcggcagc agctgggcaa gtttagccag caggcgcaca agggaggtgc cgtagtgggc
     5281 cttttgataa tccagccgcc ttccctctct ggccaggccc gtgcgggcgg atacttgcag
     5341 gcttttatcc agcagcaaca gggcgcacac cgcatcaaac gaacgctttg ccaaactgcc
     5401 cgccaggcgc tcatccaact gggtaagcaa actatccaag ggcaaagctg gcgcgtcgaa
     5461 ggccgccagg gcctgctccc ccaacagcgc ctgtaggctc gccagctccg caagcgcgcg
     5521 ggctatgtct ttaggcgggc ccgcaggcct gggcgcggcc agcagctgct caagcgcctg
     5581 gcgcgccgcc tgggcgctgg gcgcctgggc cagggctacc agcgccgccg ccagctcatc
     5641 gccgcgagct tgtgccgcaa ggctcaggca cagctgccgc aatgcccagg ggtgcaagcg
     5701 cagttttacc aggctcggca caggcaactg cgccttgagc aggtgcaaca gggccagatc
     5761 cggcggcagc cagagttcgc tatgggcgcc cggcaccagc aagcccaagc gcttgaggct
     5821 cacctccagg cggtcggcgg tgagcagggg gttgagatcc accagggtat cgaggtcagc
     5881 gttcaaccac aaaagacgct gctggcgctg gatcaggggc cggtgcaaca ccacccgccg
     5941 gggcgcttgg cttaggacct ggcggtttaa ctggcccaag gcgagggcaa agcggtcgct
     6001 aaaaatcccc cgcagggttt tttcatccag gccaaattta ggcgccaggg cggtgaaggg
     6061 gtaatacaag cccaattgcc acaggcgctg ggccaggcgc gtggtggtgc ggtgggcctc
     6121 ggcaatattg gccaccggct cgttgaaggt gactttacag tccttacaac gcaggcggcg
     6181 gcgggcgatc tccagcagca ccgctttgtt ctcaatcggc aggtcgcgga tgcgccacac
     6241 caggcggcca taacccacca ccgctttgct gccgcaggcg ctgcacgcca cagctttagg
     6301 ctcggtttcc aggccgatgg tgaggcggtt ggcctgctcg tgcatatacc gcaggctcac
     6361 cccctttaaa ggcatactcc aaagcggccc acctgcggcc ataacaccac ctcatcaatc
     6421 aggatttttt tatgtatttt ataggcgata actcgcccgt tatctatgaa ttaagccata
     6481 aataaaccgt ttaaattcgc ttaagcctag caaagcatca agtatatcca taaattattc
     6541 cgcaatagtg caagcgccca agcgccttgg caagcgcggg cgttgtgcta acctccccaa
     6601 cacctaagca caccccgccc gcctgggcgg acggttttat ttgcgccttt cttcggagct
     6661 gcccacctca atgaaacact tcacttggtc ctttatcatc accatcgttc tgatggccct
     6721 cgccggctgg tggggctata gccggggcgg cctgcccggc gccctgcaag cgctactcat
     6781 caccagcatt ttggcggtga tggaagtctc cctctcgttt gataacgcag tagtgaacgc
     6841 ctcggtgctg cgcacttgga acgccttctg gcaaaaactc ttcttaaccg tcggcatact
     6901 gattgcggta ttcggtatgc gcctggtgtt ccccatcgcc atagtggcag ttgccaccgg
     6961 ccttggggtt atggaggtta tggacctggc cctgaataac ccggatgagt actcccgcca
     7021 gctcaccgat caccacccgc aggttgcggc cttcggcggt gccttcttaa tgctggtatt
     7081 tttaaacttc ttgtttgatg aggaaaaaga cgtccactgg ctcggctgga tcgaagagaa
     7141 attcggcagc cacggcaacg aaggcctctc cacgctgctc accatgctgg cggtctacgg
     7201 cgcctcaacc ctagtgcccg aagccattaa gttatcggtg ttgatgtccg gtatcggcgg
     7261 tatcgccatc tatctagcgg tgaactacct cagcggtttg ctcgaagcta aagaagaagt
     7321 cagccacacc ggcgaagtca ttttaaaagg cagcatcggc ggctttttgt acctggaagt
     7381 gctcgatgcc tcgttcagtt ttgacggcgt gatcggcgcc tttgcgatta ccaaagatgt
     7441 ggtgattatc atgatcggcc ttgcgatagg tgcgtttttc gtgcgctcca tgaccatctt
     7501 tttggtccgc caaggcacct tggaggccta tgtttaccta gagcacggcg cccactacgc
     7561 cataggtatc ctggccgcca ttatgttatt gagcgtcact taccatattc ccgagtggtt
     7621 taccggcctc tccggtgtgg cgtttatcgg cttgtccctc tggtcgtcca tccgccaccg
     7681 ccgcctagag gcagctaagg cctagggcgc gcagcatggc gatcagcctg cgcaaagggc
     7741 gcaatttaaa cctctccaaa accgatccca gtctgcgcca cctgcggctg gggctggggt
     7801 ggaacgccaa cgccgataag gacgcagact ttgacctgga cgccagcgtt tttatgctca
     7861 ccaccgaggg caaggtgcgc ggtgacgggg actttatttt ttacggccaa ctgcgttcaa
     7921 gctgcggcgg tgtggagcac acaggcgata accgcaccgg ccttggcgat ggcgacgatg
     7981 aaatgctcag tatccggctg gccaaaatcc ctctggagat tagccgcctg gtggtggccg
     8041 tcaccatcca cgaggccgag gcgcgcgggc aaaattttgg caaggtggaa gacgcgttta
     8101 tccgcctggt taatttggac acgggaaccg aactggcccg cttcgatctg accgaggatt
     8161 attcaaacca gacggcgctg atttttggcg agctgtaccg cgctggcgat gattggaaat
     8221 tcaaggccgt ggggcaaggg tttgcgggcg atttgcgcgc cctggctctg cttcacggcg
     8281 tggatgtgca gtagcgccct ggccttcaag ccttaggcgc gccgcataaa caagttgtat
     8341 tgattgactg aacttactta aaccgcacta ccctcaacct ctaggagcac tgattatggc
     8401 tattagttta caaaaaggcg gcaacgtcaa tttaagcaaa gaagccccaa gcctgaaaaa
     8461 actcatcgtc ggcctcggct gggacccacg ggccaccgac ggcgccgcct tcgacctcga
     8521 cggcagcgcc ttcctgctca aaggcgacgg caaagtgcgc tccgacgccg actttatctt
     8581 ctacaacaac ttaaaatccg ccgacggctc catcgtgcac ctaggcgata acaccaccgg
     8641 ccaaggcgat ggggacgacg agcaattaac cattgaccta ggcgtagtac ccgccgacgt
     8701 tgagcgcgtg gtggtggctg taaccatcca cgacgccgaa gcgcgccgcc agaacttcgg
     8761 ccaagtgggc aaagcctata tccgcgtgtt aaacgccgac ggcaacaaag aaatcgcccg
     8821 ctttgacctc tctgaagacg gctccactga aaccgctatg atcttcggtg aagtctaccg
     8881 cgctggcgcc gagtggaaat ttaaagccgt cggccaaggc tttgccggtg gccttgggcc
     8941 cttggcgcgc tcttacggca ttaacgccta agggcttcct ccctgcaccg ccagtatccg
     9001 gcttgccggg tctaggcggc gcagggtttt accgtctgcc tgcgaggcca agagcatgtt
     9061 agaactgcgc cccaactgcg agtgctgcga taaagacctg ccccccgaca gcctcgacgc
     9121 catgatctgt acctttgaat gcaccttttg ccgcagctgt gcgacaactg tgttagcggg
     9181 cacctgcccc aactgcagcg gcaacttagt ggcccgcccc gtgcgcccgg ccgcccagct
     9241 gcaccgctac ccagcctcga gcctgcgggt agttaaaccc ggcggctgcc caagccctta
     9301 aatttatcaa ccccatttgc cccacggagt aaggttatga ctgtgtttac ccccactggc
     9361 gacgtcgatc cgtttttgca tgttgccatg cagcggggcg acaaaattta ttgcgaatcc
     9421 ggcgccatgg tgatgatgga cgccaccctg gatttaaaag gcaaaatggg cggcggcttt
     9481 ggcgcagccc ttatgcgcac cttcgccaac ggcgaatctt tttttcaaca gcatattgaa
     9541 gcggtgcgcg gcgacggcga ctgtttatta tcccccgcca tgcccggcgc catggatgta
     9601 gttgaagtgg gtaaaaaccg ctatttatta aatgatggag cctttgtggc cgccacttcc
     9661 ggggtg
//
LOCUS       DAOQGS010000092         9647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3317_length_9647_cov_5.272415, whole genome shotgun sequence.
ACCESSION   DAOQGS010000092 DAOQGS010000000
VERSION     DAOQGS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_9647_cov_5.272415"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PKZ52_05115"
     CDS             <1..1269
                     /locus_tag="PKZ52_05115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNG59185.1"
                     /translation="APAVVTPPQQAPAVAEANKKTASAETSSIRVGIDKIDSLINMVG
                     ELVITQSMLGQLGSELNLAAAPKLMEGLSQLAQNTRELQESVMRIRMLPISFAFSRFP
                     RMVRDLGQTLGKKIHIELNGENTELDKTVMEKIGDPLVHLVRNAVDHGIELPEVRLAK
                     GKPEEGHILLNAYHQSGNVIIEITDDGKGLDREAITQKAIEKNIISAQEAANFTDEQV
                     YDLIFQPGFSTAQTVSDVSGRGVGMDVVKRNIQALNGVVEISSQPNKGSRIRIRLPLT
                     LAILDGQLVRVNDHIYIFPLVAIVESVQHRVERMRHIAGGCSVFKLREDYVPVLHLKD
                     VFNLEGDRQRDEPQLMVVVESEGEKVGIVVDELLAQQQVVIKSLEQNYKKVEGVSGAT
                     ILGDGTVALILDIQGIVHLAGLRQAHQHIA"
     gene            1342..1881
                     /locus_tag="PKZ52_05120"
     CDS             1342..1881
                     /locus_tag="PKZ52_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNG59186.1"
                     /translation="MQTPSFDLHHNHAEQNAASATSQYLTFIMNHEEYGVDILCVQEI
                     RGWETATPLPNAPKHIKGVINLRGTIVPIIDLRQCFGLPAIDYTAVTVVIVLKVQQAG
                     VSRVVGIVVDGVSDVYSLADKDLRQAPDLGHAVDMHFVKGLVSIENKMVILLNIDQLL
                     NIEDMPNLSGLHEHLAGVC"
     gene            1899..4619
                     /locus_tag="PKZ52_05125"
     CDS             1899..4619
                     /locus_tag="PKZ52_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG59187.1"
                     /translation="MFNFFKSGEHKSDSNCYEHAQLVEALYQAQAVIELDPDGNILSA
                     NELFLSVVGYSLAEVQGRHHRIFIFKDEQQSPEYSRFWARLNSGERISAVFKRVDKLG
                     KVIWLQASYNPIRDAAGRVYKVVKFAQDITKTRNMANLASALKSCQANVMLADNDLSI
                     VYTNDTVVEMLRARETQLKTALPRFSVANLVGSNVDVFHAHPSHQRTLLQGLREPYNT
                     EIKVAGLTFGLIATPWFDDSGVRLGTLVEWQDKTDQLARDEAERKINVENVQIRRALD
                     VCDTSVMIADANLNIGYMNQSVRSMMERNERALREILPGFNVANLLGSSIDQFHKNPQ
                     HQRKLLENMHSEYRTDINVAGLIFGLIATPIYDGTGKRMGTVVEWDDKTERVARENEA
                     KRLADENARVRQALDNVSTNVMIADENANIVYLNSAVGAMMRKAESDLRRALPNFDAN
                     RLMGMNIDAFHRNPAHQRDILKNLRTTYHGKAEVGGRTFTVIANPVMQGDKRIGTVVE
                     WADRTAEIAMEKEIDAMILAAGQGDFTKQISLDGKEGFFASISKGLNSLVSTVEIALN
                     DMLRMLGAMAKGNLSERITREYAGVFGQLRQDANFTADKLTEIISKIRQSAGSIHTAA
                     NEIAQGNADLSQRTEEQASSLEETASSMEEMTSTVRQSAENAQKANELALQAQSKAQT
                     GGVVVNRAVQAMEDINAASKKIADIISVIDEIAFQTNLLALNAAVEAARAGEQGRGFA
                     VVAGEVRNLAQRSAGAAKEIKELIRDSVIKVQDGRELVNESGKTLGEIVSAVEVVCQM
                     MRDIASAAREQTAGIEQVNTAVSQMDEMTQQNAALVEEATAASQSMVDQANDMGQLVS
                     FFNVGQVAGMAAATHAARHVAPVKAGAKKAKAVKPVKSVHPSASDEWEDF"
     gene            4954..5799
                     /locus_tag="PKZ52_05130"
     CDS             4954..5799
                     /locus_tag="PKZ52_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNG59188.1"
                     /translation="MTEFLSLQQRDREFPMSQANFNLIKNLAYTWTGIKLSDHKENMI
                     YGRLARRLRALNMADFDQYCALLQSEDEQEKREFINSITTNLTSFFREPHHFDFLKNQ
                     LLPGLLQRNQSTRKIRIWSAGCSTGEEPHSIAMVVASIAELKHWDVKILATDLDSNVL
                     ERASGGIYSLGRFDAFPSSYKQFIKKSADGEQVKIADSVRGLIRFNQLNLLQAWPMKG
                     SFDIIFCRNVVIYFDLETQRKLFNRYADKMVDKGHLFIGHSENLHKISDRFLSLGRTI
                     YEKIK"
     gene            5834..6469
                     /gene="cheD"
                     /locus_tag="PKZ52_05135"
     CDS             5834..6469
                     /gene="cheD"
                     /locus_tag="PKZ52_05135"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HNG59189.1"
                     /translation="MATVILPKPLAGFEHVYRNWDGHWQCPAAKILPGECYVSTQEEV
                     IHTVLGSCISVCIRDRKLGVGGMNHFMLPVQEGERGITRQNSINPALCYGNWAMEFLI
                     NAILKQGGRREHFEIKMFGGGRVLTGMTNIDIGARNIEFAREYLRQEKFTLAAEDVGD
                     VCPRKVVYFPKTGAVKLRRLVHTANDSIERRERDYLLSMNKKPTTTDVELF"
     gene            6471..7535
                     /locus_tag="PKZ52_05140"
     CDS             6471..7535
                     /locus_tag="PKZ52_05140"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNG59190.1"
                     /translation="MPKAIKVLIIDDSALLRAILREMINSDRRFSVVAEAKDPYEARE
                     LIKQHDPDVLTLDVEMPRMNGITFLSNLMRLRPMPVVMISTLTQEGAPATLEALELGA
                     VDFLPKPQSGGDSALEVYRELILEKLYWASKANVVVDIRAGAKPKNDLASLTHNRNLK
                     AGFICAIGASTGGTEAIKHVLEALPDYSPPIVITQHIPAAFSTSFARRLDSLCQIKVY
                     EAEHNQAIEAGAAYLAPGHSHLRLLKTPKGYVCRLDQADPVNRHRPSVEVLFDSVAGQ
                     AGANSMGIMLTGMGADGAQALLRMRQAGAATVAQDEASSVVWGMPGAAVKCGAVEKIL
                     PLEKIPRQILQYAFNLKALG"
     gene            complement(8307..>9647)
                     /locus_tag="PKZ52_05145"
     CDS             complement(8307..>9647)
                     /locus_tag="PKZ52_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252959.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNG59191.1"
                     /translation="EQGLGWSNAFGSGKGVHTTTSGIEGAWKPNPTQWDNGYFDMLFG
                     YDWELTKSPAGAHQWTPKNCKPEHMIPDAHDPSKRFPPMMTTADLSLRFDPAYEKIAR
                     RFHQNPAEFADAFARAWFKLTHRDLGPRSRYLGKLVPAETLLWQDPIPAVNHPLVDAQ
                     GVVQLKQRVLASGLSISALVATAWASASTFRGSDKRGGANGARIRLSPQNSWAVNQPA
                     QLAQVLATLTGIQEAFNLEQTGGVQISLADLIVLAGSAAIEEAAQRAGVSIQVPISLG
                     RMDASSEQTDVESFQVLEPVADGFRNYAAKGFESIAAELLIDKAHLLTLSAPEMTVLV
                     GGLRVLNANTNQNPQGVFSQRPQTLSNDFFTHLLDMNIKWHKVPGEHYLAGKNRRSGE
                     LVWTASVVDLIFGSNSQLRAIAEVYAQNDNSEKFVKDFVAAWTKVMNLDRFDLQ"
BASE COUNT         2423 a         2092 c         2672 g         2460 t
ORIGIN      
        1 gctcccgctg ttgtaacacc gccgcaacag gcgcctgcag tggctgaggc aaacaaaaaa
       61 accgcctcgg cggaaacctc ttccatccgt gttggcatag ataaaatcga cagccttatt
      121 aatatggtgg gtgagttggt gattacccaa tccatgttgg gccagctcgg cagtgaatta
      181 aatctagcgg ccgcgcctaa attaatggaa ggcttaagcc aattggcgca aaacactcgc
      241 gagctgcaag aaagtgttat gcgcattcgc atgctgccaa ttagttttgc ttttagccgt
      301 tttccgcgca tggtgcggga tttgggccaa acattgggga aaaaaattca cattgaatta
      361 aatggcgaaa ataccgagct tgataaaacc gtcatggaaa aaattggcga cccgctggtg
      421 cacttggtgc gcaatgcggt ggatcacggt atagagctgc ccgaggtgcg cttagcaaaa
      481 ggcaagcctg aggaggggca cattttactt aatgcctatc accaaagcgg caacgtaata
      541 attgaaatta ctgacgatgg caaaggcctg gaccgtgaag cgatcaccca aaaagccatc
      601 gaaaaaaata ttatctccgc ccaggaggcc gccaatttta ccgatgagca ggtgtacgat
      661 ttaatttttc aacctggctt ttccaccgcg caaacagtga gtgatgtgtc cggccgcggc
      721 gtcggcatgg atgtagtgaa gcgcaatatt caggccctta acggtgtggt ggaaatttcg
      781 tcacagccga acaaaggcag ccgaattcgc atccgcctgc cgctgaccct ggcgattctc
      841 gacggccaat tggtgcgggt gaacgaccat atttatattt tcccactggt agcaattgtg
      901 gagtcggtgc agcaccgggt tgagcgtatg cgccatatag ccggaggttg cagcgttttt
      961 aaattgcgcg aagattatgt gccggtattg cacttaaaag atgtgtttaa tttagagggc
     1021 gaccggcagc gggatgagcc ccagttaatg gtggtagtgg aatcggaagg tgaaaaagtg
     1081 ggaattgtgg tagatgagct tttggcccaa cagcaagtgg taattaaaag cctggagcaa
     1141 aattacaaaa aggttgaagg tgtttccggc gcgaccatat taggcgatgg caccgtggcg
     1201 cttattttag atattcaggg gattgtgcat ttggcgggcc tgcggcaggc ccatcaacat
     1261 atcgcctaaa aaataatggc gcctggggct gttggctttg gcgctggatg caattaattg
     1321 aatgcttact gaggatgccc tatgcagacc ccctcatttg atttacacca taaccacgcg
     1381 gagcaaaacg ccgccagcgc caccagccaa tatctaactt ttattatgaa tcatgaagaa
     1441 tatggcgtgg atatactgtg cgtgcaggaa attcgcggct gggaaacggc aacgccactg
     1501 cccaatgcgc ccaagcatat taaaggggtt attaatttgc gcggcaccat agtgcccatt
     1561 attgatttgc ggcagtgctt tgggctgcca gctatcgatt atacggccgt tactgttgtg
     1621 attgtattaa aagtgcaaca ggctggggtt tctcgcgtgg tggggatagt ggttgacggt
     1681 gtttcagatg tttattcact ggcagataaa gatctgcggc aagcaccgga tttggggcat
     1741 gcagttgata tgcattttgt aaaaggcctt gtgagtatcg aaaataaaat ggttatactt
     1801 ttaaatattg atcagttgct caatattgaa gatatgccaa atttgtctgg cttgcatgaa
     1861 catcttgctg gcgtatgtta atttaacggg aggatcccat gtttaatttt tttaaaagcg
     1921 gtgaacataa aagcgattca aattgttatg agcatgccca gttggttgaa gctctatacc
     1981 aggctcaggc ggttatagag cttgatccgg atggaaatat tctaagcgcc aatgaattat
     2041 ttttatcggt tgtaggatat tcacttgctg aggtgcaggg gcggcatcac cggatattta
     2101 tatttaaaga cgagcagcag tcgccggaat atagtcgttt ttgggcgcgc ttgaacagcg
     2161 gtgagcggat aagtgcagtg tttaagcgcg ttgataagtt gggtaaggtt atttggctgc
     2221 aggctagtta taacccgatc cgagatgcgg ctggccgagt ttataaagtg gtcaaatttg
     2281 cccaggatat tactaaaacg cgcaatatgg ccaatttggc gtctgcactt aaatcctgcc
     2341 aggccaatgt tatgttggcg gataatgact tatccatcgt ttatacgaat gatacggtag
     2401 tggagatgtt gcgggccaga gagacacaat taaagactgc tttgcccagg ttttcggtgg
     2461 ctaacttggt gggttccaat gtggatgttt ttcatgccca ccccagtcat caacgcacat
     2521 tattacaggg attgcgcgag ccttataata ctgaaattaa agtagctggt ttaacctttg
     2581 gccttattgc caccccctgg tttgatgact ctggagtacg gcttggcacg ttggtggagt
     2641 ggcaggataa aacggatcag ttggctcgcg atgaggcaga gcgaaaaata aatgttgaaa
     2701 acgtccagat tcgtcgtgcg ctggatgtgt gtgatacctc tgtcatgatt gcagacgcta
     2761 atttaaatat tggctatatg aatcagtctg tacgcagcat gatggagcga aatgagcgcg
     2821 cgttgaggga gatattgccg ggctttaatg tggccaattt gcttggaagc tcgattgatc
     2881 aattccacaa aaatccccag catcagagaa aactactaga gaatatgcat tctgagtacc
     2941 gaactgatat taatgtggcg gggttaattt ttggattgat agcaaccccc atttacgatg
     3001 gcaccggtaa gcgaatggga actgtagtgg aatgggacga taaaactgag cgtgttgcac
     3061 gggaaaatga agctaagcgc ctagctgatg agaacgcacg agttcgtcaa gcgctggata
     3121 atgtaagtac gaatgtaatg attgcagacg aaaatgccaa tattgtgtat ttaaactccg
     3181 cagttggcgc catgatgaga aaagctgagt cggatctcag aagagcatta cctaattttg
     3241 acgctaatcg tttaatgggt atgaatatag atgctttcca ccgaaatcca gctcatcaac
     3301 gagatatatt aaaaaacctg cgcacaactt accatggtaa ggctgaggtg ggcggtcgga
     3361 cgtttacggt aatcgctaac ccagttatgc aaggcgataa aagaatcggc acagttgtgg
     3421 agtgggcgga tagaacggct gaaatcgcca tggaaaaaga aattgatgca atgattttgg
     3481 cggccgggca gggggatttt accaagcaga tttccctgga tggcaaagag ggcttttttg
     3541 ccagtatcag caaagggtta aattccctgg tgagcaccgt tgagattgcg ctaaatgata
     3601 tgttgagaat gttgggcgcc atggccaagg gtaatttgtc tgagcggatt acgcgggaat
     3661 acgctggtgt atttggccag ttgcgccaag atgctaattt taccgccgat aagttaacag
     3721 aaattatcag caaaattcgc cagtcggcag gttccattca taccgccgcc aatgaaattg
     3781 cgcagggtaa tgccgattta agccagcgca ctgaagagca ggccagctct ttggaagaga
     3841 cggcttccag tatggaggag atgacgagta ctgtgcgtca gagtgctgaa aatgcacaaa
     3901 aagctaatga attagctctg caggcacaga gtaaggcgca gacgggagga gttgttgtta
     3961 accgggccgt gcaagcaatg gaggatatta atgccgccag taaaaaaatc gccgatatta
     4021 ttagtgtgat tgatgaaatc gctttccaaa ctaatttgtt ggcgttaaat gcggcagttg
     4081 aagcggcccg tgccggtgag cagggacgcg gctttgctgt ggtggcaggc gaagtgcgca
     4141 atcttgccca gcgctcggcg ggggccgcaa aagaaattaa agaattaatt cgcgatagcg
     4201 ttattaaagt gcaagacgga cgggaattgg tcaatgaatc gggtaaaacc ctcggtgaaa
     4261 ttgtctctgc cgttgaagtg gtgtgccaga tgatgaggga tattgccagc gcggcgcggg
     4321 agcaaaccgc aggtattgag caggtgaata cagctgtttc acaaatggat gaaatgacgc
     4381 aacaaaatgc cgctttggtt gaagaggcca ccgccgctag tcaatcgatg gtggaccaag
     4441 ccaatgatat ggggcaattg gtgagctttt ttaacgtagg ccaggtggcg ggtatggccg
     4501 ctgccaccca tgctgcgcgg catgtggcgc cggttaaagc gggtgctaaa aaagccaagg
     4561 ccgtaaagcc agtaaaatca gtgcatccat ccgcgtcgga tgagtgggaa gatttttaat
     4621 ttttgtgttg ttcagtacct atttattggt gctggatgga attcttgtgc cgataggaaa
     4681 cttcaggcga gtgtttagtg ctgcaccgca aagcgattat ggctaagtcc tcctcaattt
     4741 actgcccgtg gatagagagt tataaggtgt attattaacc cggcggcatt tagtgccagg
     4801 ttaataaatc gttttagtgt tcttagatag gcacgtcagg ctcagtggtt ttttgaagca
     4861 caacatgttc ggccccttac tgcctctgtt gtttggcccg cgtgaaggtg gagctaaatg
     4921 tttggcattt aaaccgagga ctcagcacgg cctatgacag agtttttatc cttgcagcag
     4981 cgcgatcgtg aatttcccat gtcgcaggcc aattttaatt taattaaaaa tttggcttac
     5041 acatggacag ggattaaatt gtcggatcat aaagaaaaca tgatctacgg gcggcttgcc
     5101 cgcaggctgc gggcgctgaa tatggcggat tttgaccagt attgtgcgct gttgcagtct
     5161 gaagatgagc aggagaagcg ggagtttatt aactcgatta ccactaatct cacgtcattt
     5221 ttccgcgagc cccatcactt tgatttttta aaaaaccagt tgttgccggg gcttttgcag
     5281 cgcaaccagt ctacccgtaa aattcgtata tggtcagctg gctgcagcac tggcgaagaa
     5341 cctcactcca tcgccatggt ggtggcttct attgccgagc ttaaacattg ggatgttaaa
     5401 attcttgcca cagatcttga ttctaatgtg ttggagcggg ccagtggcgg catttatagc
     5461 ttgggccgct ttgacgcgtt tcccagtagc tacaagcagt ttattaaaaa atcggcggat
     5521 ggcgagcagg taaaaattgc tgatagtgtg cgcgggttga ttcgttttaa tcagctgaat
     5581 ttattgcagg cctggcccat gaagggctcg ttcgatatta ttttttgccg caatgtggtg
     5641 atttattttg atttggaaac ccagcgaaaa ctgttcaacc gctacgcgga taaaatggtg
     5701 gataaaggcc atttgtttat cggtcactcg gagaatttgc ataaaatctc tgaccgcttt
     5761 ttaagccttg ggcgcaccat ttacgagaaa ataaaataag ccacctgggg tggtgcgagt
     5821 gattaaatag tgtatggcga cagtaatatt acctaagcct ttggcgggct ttgagcatgt
     5881 ataccgcaat tgggacggcc attggcaatg cccggcggct aaaatattgc ccggtgagtg
     5941 ttatgtgagc actcaggaag aagtgatcca caccgtgttg gggtcttgta tctctgtgtg
     6001 tattcgcgac cgcaaattgg gtgtgggcgg tatgaaccat tttatgttgc ccgtacagga
     6061 gggtgagcgg ggcattacgc ggcaaaattc cattaacccc gccctgtgtt acggcaactg
     6121 ggctatggag tttttgatta acgctattct aaagcagggc ggccgccggg aacattttga
     6181 gataaaaatg tttggcgggg ggcgggtatt aaccggcatg accaatattg atataggtgc
     6241 gcgcaatatt gagttcgccc gtgaatattt gcggcaggaa aaattcaccc tggcggcgga
     6301 agatgtgggg gatgtatgcc cgcgtaaagt tgtgtacttc cctaaaaccg gcgccgttaa
     6361 attgcgccgc ttggtgcaca cagccaatga ttcgattgaa cggcgcgagc gggattattt
     6421 gttgtcgatg aataaaaaac ccactactac cgatgtggag ttattttagt atgcctaaag
     6481 caatcaaagt gcttattatt gatgactcgg cacttctgcg cgctatcttg cgtgaaatga
     6541 ttaattccga tcgacgtttt tctgtagtgg cggaagccaa agacccctat gaggcgcggg
     6601 agctaataaa acaacacgac ccggatgtgt tgaccttgga tgtggaaatg ccgcgtatga
     6661 atggcattac ctttttaagt aatttaatgc gtttgcggcc catgccggtg gtgatgattt
     6721 caacccttac tcaggaaggg gcgccggcaa ccctggaggc gctggaatta ggtgccgtgg
     6781 attttttgcc aaaaccccag tccggtggcg atagtgcatt agaagtgtac cgcgagctta
     6841 ttttagaaaa actttattgg gcgtccaaag ccaatgtggt tgtggatatt cgggcgggcg
     6901 ctaagcctaa aaacgatctt gcgagcttga cccataatcg caatttaaaa gccggattta
     6961 tttgtgccat cggcgcatcc accggcggca cagaagcgat taaacacgtg ctggaagcat
     7021 tgcccgacta ttctccaccg attgttatta cccagcatat accggcggca tttagcactt
     7081 cattcgcccg ccgcttggat agcctgtgcc aaataaaagt gtatgaggcc gaacacaatc
     7141 aagccattga ggcgggggct gcttatttag cgccgggcca tagccatttg cgtctattaa
     7201 aaacgccgaa aggttatgtg tgccgtttgg atcaggccga tccggtaaac cggcataggc
     7261 catctgtgga agtattattt gattcggtgg ccgggcaggc cggtgccaac agcatgggta
     7321 ttatgctcac cggaatggga gcggacggcg cgcaagcact tctgcgcatg cggcaggccg
     7381 gtgctgccac agttgctcag gatgaagcga gttcagtggt gtggggtatg ccaggggcgg
     7441 cggtgaaatg tggcgcagtg gaaaaaattc tgccgctgga aaaaatccct cgtcaaatcc
     7501 tgcaatacgc gtttaattta aaagccttgg ggtgaggaat agcacttaat tagcggtgct
     7561 ttaaaaatta tttaactatt cagcaacttt ttatgtggct ggaggtaatt cctgcactga
     7621 ccgggaacta tcgcatgtgt gttttagtgc tgtgccctcg caatccagcg tccgcagcgg
     7681 tgactacagg ttagtgcaga cacgccgtga atacatccgt gtaggctcgt caaaaaggtc
     7741 cctctttttg acggtctgca ctaacctgta gtcaccactg ctacaccgcg aatcgattat
     7801 gccagatggc caaattctcc tgaatttgcc gcccgcgcgg aagaggggag taaagtgtgc
     7861 tgaatagtta caaaattatt tactctgcac taactgccgc cgggttaata attgggataa
     7921 tgtgccgtag gctgggctat ggatcctgcg acttcgcgca ggatgacaat cctagacact
     7981 gcaaggcgag tcggcaaaac gcgtcatcct gcgcgaagtc gcaggatcca aacccaaaag
     8041 ccagcgagaa caatgttcaa cagccagtta ccttttttgt aagaagccgc cgattaacag
     8101 cttgttgaaa tacgtaggcg gcgacgcgct ggcaaggcaa atttggcgaa aaaatcctca
     8161 tttactgcgt gtaaactccg gttttttcgc caaatttaac gccgccaacg ctcgcaaggt
     8221 agtatttcaa caagctgtta aatcaggcag cggaaatcgt gtgccgcccc agcctgtcac
     8281 aaactggggt tggttttccg ttatgtttat tgcagatcaa accgatccaa attcatcacc
     8341 ttagtccagg ctgccacaaa atccttaacg aatttttcgc tgttgtcgtt ttgtgcataa
     8401 acctcggcaa tggcccgcag ttgtgaatta gaaccaaaaa ttaaatctac cacactggcc
     8461 gtccacacca attccccgct gcggcgattt ttgcccgcta aataatgctc gcctggcacc
     8521 ttgtgccatt tgatattcat atccaataaa tgggtgaaaa aatcattgct aagggtttga
     8581 ggcctctggc tgaatacacc ctgggggttt tggttggtat tggcatttaa tacccgcagg
     8641 ccacccacca gcacggtcat ttcaggggcg ctcagggtga gtaaatgggc tttatcaatt
     8701 aataattcag ccgctatgct ctcaaaccct ttagccgcgt aattgcgaaa gccgtcggcc
     8761 acaggttcaa gcacctggaa tgattccacg tccgtctgct cgctgctggc atccatacgg
     8821 ccaaggctaa taggtacttg aatagatact ccggcccgtt gggcggcttc ttcaatggcg
     8881 gcgctgcccg ccagcacaat taaatccgcc agggatattt gcacgccgcc ggtctgctcg
     8941 aggttaaagg cttcttgaat gccagtgagg gtggcgagca cctgggctag ttgcgcaggc
     9001 tgatttaccg cccagctgtt ttgcgggctt aagcgaatgc gcgcgccatt ggcgccgccg
     9061 cgtttgtcgc tgccgcgaaa ggtcgaggcg ctggcccagg cggtggccac cagggcggag
     9121 atggatagtc ctgacgccag tacccgctgc tttaactgga caacaccttg ggcgtccacc
     9181 agggggtggt ttaccgcagg gatggggtct tgccagagca gggtttcggc gggcactaat
     9241 ttacccaggt agcggctgcg ggggcctagg tctcggtggg tgagtttgaa ccaggcgcgg
     9301 gcgaaggcat cggcaaattc cgctgggttt tggtgaaagc ggcgggcaat tttttcatag
     9361 gccgggtcaa agcgcaggga gagatccgcg gtggtcatca tgggcgggaa gcgcttggat
     9421 gggtcgtggg cgtccgggat catatgttcg ggtttgcagt ttttcggcgt ccactggtgg
     9481 gctccggcgg ggcttttggt gagttcccag tcgtagccga acaacatgtc aaagtagccg
     9541 ttatcccatt gcgtggggtt tggtttccag gcgccttcaa tgccgctggt ggtggtgtgc
     9601 acgcccttgc cgctgccaaa ggcattgctc cagcccaggc cctgctc
//
LOCUS       DAOQGS010000093         9611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3332_length_9611_cov_4.830159, whole genome shotgun sequence.
ACCESSION   DAOQGS010000093 DAOQGS010000000
VERSION     DAOQGS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_9611_cov_4.830159"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /gene="lspA"
                     /locus_tag="PKZ52_05150"
     CDS             <1..387
                     /gene="lspA"
                     /locus_tag="PKZ52_05150"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNG59192.1"
                     /translation="EYEGQSQPYTFFFSLSLHYNHGAAFSFLAQEGGWQRWFFMGIAA
                     AVSLGLGYWLARLSRQKVIEALGLSLVLGGALGNLYDRVTLGYVVDFLLFHWGDAYFP
                     AFNVADMAICAGAAALVWDSLRKKGE"
     gene            390..842
                     /locus_tag="PKZ52_05155"
     CDS             390..842
                     /locus_tag="PKZ52_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNG59193.1"
                     /translation="MTTSLPIQAGMQVTLFFALRLPDGEVIDSNFDQQPATFVMGDGN
                     LLPGFERVLMGLHSGERKDFVIAPEHAFGQRNPNNLQKIERRLFSTELNLEPGLVVNF
                     ADAQNTELPGVVADVDDRLVTVDFNHPLAGQAIEFDVHILNVQPATSH"
     gene            855..1787
                     /gene="ispH"
                     /locus_tag="PKZ52_05160"
                     /gene_synonym="lytB"
     CDS             855..1787
                     /gene="ispH"
                     /locus_tag="PKZ52_05160"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNG59194.1"
                     /translation="MQIKLANPRGFCAGVDRAIDIVNRALDVFGAPIYVRHEVVHNKF
                     VVEQLKERGAVFVDELVDVPDDVIVIFSAHGVSQSVRQEAAARGLRVFDATCPLVTKV
                     HLEVAKYSEDGWECVLIGHAGHPEVEGTMGQYTNAHGGAIYLVENEADVAVLPVKNPE
                     KLAYVTQTTLSMDDTARVIDCLRARFPNIQGPRKDDICYATQNRQDAVKQLALETQVV
                     LVVGSPNSSNSNRLRELAERCGAKAYLIDGPQDLQAHWFAQANTVGITAGASAPEVLV
                     QSVIHGLKQYGAQMPEELAGRVENVSFSLPKELR"
     gene            2001..2306
                     /gene="ihfB"
                     /locus_tag="PKZ52_05165"
     CDS             2001..2306
                     /gene="ihfB"
                     /locus_tag="PKZ52_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNG59195.1"
                     /translation="MTKSELIDRIATAQDQLSPKDVEIAVKLLLEFMSQSLAKGERIE
                     IRGFGSFALHYREPRVGRNPKTGEAVALEGKYVPHFKPGKEMRDRVNESIKQKKRRR"
     gene            2569..2910
                     /locus_tag="PKZ52_05170"
     CDS             2569..2910
                     /locus_tag="PKZ52_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59196.1"
                     /translation="MYESKRQNPITQAKFIRRLFRQLAWVFILLAFSLLIGMSGYVRF
                     EGLSWVDAFLNASMLLGGMGPVNPPQSFAGKLFAGFFALYAGLVFIVAAALLFTPLLH
                     RLLHHFHWDQE"
     gene            3333..3869
                     /locus_tag="PKZ52_05175"
     CDS             3333..3869
                     /locus_tag="PKZ52_05175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNG59197.1"
                     /translation="MKIIAFAASSSRQSINKQLITYAAGLLQGVEVEILDLNDYELPL
                     FSVDREAQLGHPQLAKDFLAKIAASQGLIISFAEHNSTYTAAYKNLFDWCSRLEKKVF
                     ADKPAVFLATSPGGRGGASVLSEALASAGRFGAQVVASLAVPNFADNFDAAAGVLTHA
                     ELNQQLVAAVGALQAARG"
     gene            complement(3859..5175)
                     /locus_tag="PKZ52_05180"
     CDS             complement(3859..5175)
                     /locus_tag="PKZ52_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNG59198.1"
                     /translation="MSLDVISVNYLGKTVGAASFDTQTGLGAFEYTAEFLDTGIELSP
                     LKMPLAKRIYTFPELDHATFKGLPGLLADSLPDDFGNAVLNAWMASKGKQAEQITPLQ
                     RLKYTGKRGMGALEYAPATTLKNLNASQPVAIESLVTIAQEVLDSRANFKLELNKQGQ
                     EDREAMLALLSVGMSAGGARPKAVLAFNKDFTQVRSGQTKAPAGFTHYLMKFDGVREH
                     NQNNETFGDPMGYGAMEYVYYLMATSCGIHMMPCSLLPEGKRRHFITQRFDRIGNEKL
                     HIQTLNGLAHVNYKMPGSFSYAEIFAVARQLRLTALDAEQLFRRMAFNIIARNHDDHS
                     KNSAFILRDKTWQLAPAYDLAYSYKPGSPWVNSHWMSLNGKRDNFTRQDFYSLESLSP
                     LFGRAKINTIIDEITQQVANWPSLAREHEVPATLSREIAKNLRLNL"
     gene            complement(5183..5521)
                     /locus_tag="PKZ52_05185"
     CDS             complement(5183..5521)
                     /locus_tag="PKZ52_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG59199.1"
                     /translation="MISQSMPPAAVAEALGERLKQARLNADLTQARLAELAGVTRKVV
                     LNAEKGKAQLEAFVALILALNLAEHLDKFLPPQTISPVQLAKLQAKQRKRASRAERNP
                     IAHPTPTSDW"
     gene            5866..6258
                     /locus_tag="PKZ52_05190"
     CDS             5866..6258
                     /locus_tag="PKZ52_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59200.1"
                     /translation="MHTYVAFTSDMFQPFLPESAQVNPHCYGAELAWWLCQQLAGKGF
                     VTSYPNSEDWGWFIEYAVDDYEYWLCCGNVPGASNRWQIYLDCKPKGLFGRDKAPVAL
                     AEDLLTALVDLLATTSGVGELIWRDSSE"
     gene            7353..8681
                     /locus_tag="PKZ52_05195"
     CDS             7353..8681
                     /locus_tag="PKZ52_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59201.1"
                     /translation="MLLLSTEKARAVYFFDQAEIVGQLLLPEFESMLDGFVPSKEWAL
                     RTMQAVYVEINHRLLITAAVFFKVGFDDHGWIDGSWNMPLADLAKRGQAGPDLGAGPI
                     HMATFSQCPIDLYKQLLWDPEVKGRQNHLGQLKKAVSRNRLGICFVDDGQDDPNKPDN
                     LVAAMGERLQTVLHQHLNQRDDTGIKNVLAKVIDEQARIPTLVPSSEDLKERLELLEQ
                     MVQERDEQLAHLERQLAEAQQQLVQATEHSQTLSNKAEGLRNYYEHKIIRMEQQQLSL
                     AAQAQLSAASDSEIAAAVHEATQDFAELLQRREIELLYRSEREEQLLNQLREQNEHPS
                     AADERIFDYLQMLNDHGVRFVHYRAGLTPIQIPVGELAEFLKSATAYTAQKLGVTEAL
                     YNAWLSHFLMPVCSAATVNGPCAASIYRVEEPRHFIPGVSDCCTEHQPKT"
     gene            8753..>9611
                     /locus_tag="PKZ52_05200"
     CDS             8753..>9611
                     /locus_tag="PKZ52_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNG59202.1"
                     /translation="MYLSSPSPLYNLAQRAQVQPVAWSCARAANCRIWIKRDDLLHAA
                     VSGNKLFKLFHHLMQAKKCAARGVISFGGAYSNHLYALARVAKALNLPAVAIIRAGDA
                     DLNNPTLSELRAQGMQLLPISREQYRRKMEPAFSAELTTRYADFYLIPEGGAGVLGAQ
                     GFADYAEAVSAQLRAQDITPDSVWLPAGTGTSVAGLASRWHNVQAVGALAITDPKPYT
                     QAVEAWTQQLTGSAGVHRVNWHGFDRPFGKIPASILDVCREFWCETGVLFDPIYGGKG
                     VLALQKAFAQ"
BASE COUNT         2223 a         2154 c         2657 g         2577 t
ORIGIN      
        1 gaatacgagg gccagtccca gccgtatact ttctttttta gtttgtcctt gcattataac
       61 cacggcgccg cgtttagttt tttggctcaa gagggcggtt ggcagcgctg gttttttatg
      121 ggcatagcag ctgcggtgag cttaggcttg ggttattggc tggcgcgttt gtcgcggcag
      181 aaagtgatcg aggcgctggg gttgtcgtta gtgttgggcg gcgccttggg caatttgtat
      241 gatcgcgtga cgctcggtta tgtggtggat tttttattat tccactgggg ggatgcgtat
      301 tttcccgcgt ttaatgtggc ggacatggcc atatgcgcag gcgccgctgc cctggtatgg
      361 gattctctgc gtaaaaaagg cgaatgatta tgaccactag cttgcccatt caagccggta
      421 tgcaagttac tttatttttt gccctacgcc tgcccgacgg tgaggtaatc gacagcaatt
      481 ttgaccagca accagctacc ttcgtgatgg gggatggcaa tttattaccg ggttttgaac
      541 gtgtattaat ggggttgcac agcggcgagc gcaaagattt tgtgatcgcc cccgagcatg
      601 cattcgggca gcgcaaccct aataatctgc aaaaaattga gcggcggtta ttttccactg
      661 aattaaattt agagccgggt ttagtggtta atttcgccga cgcgcaaaat accgaattac
      721 ccggtgtggt ggctgatgtg gatgatcgct tggtaacggt tgattttaat caccccttag
      781 ctgggcaggc catcgagttt gacgtgcata ttctcaacgt gcagccagcc acatctcatt
      841 aggaaagttt ttgcatgcaa attaaattag ccaacccccg tggcttttgc gcaggtgtcg
      901 atcgcgccat agatattgtc aaccgcgcct tagacgtatt tggcgcaccc atttatgtgc
      961 gtcatgaagt ggtgcataac aaatttgttg ttgaacaatt aaaagagcgc ggcgctgttt
     1021 ttgtggatga gctggtggat gtgccggatg acgtgattgt tattttcagc gcccacggtg
     1081 tatcccagtc tgtgcggcaa gaggccgcag cgcggggcct gagagtattt gatgccacct
     1141 gcccattggt gactaaggtg caccttgaag tggccaaata cagcgaggat ggttgggagt
     1201 gtgtgctgat cggccacgcc gggcacccgg aggttgaggg cactatgggg caatatacca
     1261 acgcccatgg cggcgccatt tatctggtgg aaaatgaagc ggatgtagcg gttttaccgg
     1321 taaaaaatcc tgaaaaactc gcctatgtga cgcagactac tctttctatg gatgacacag
     1381 cgcgggtgat tgactgcctg cgggcgcgct tccctaatat tcaggggccg cgcaaagatg
     1441 atatttgtta tgccacacaa aaccgccaag atgcggtaaa gcagttggct ttggaaactc
     1501 aggtggtgtt agtggtgggg tcacccaata gctctaactc caaccgtttg cgggagttgg
     1561 ctgagcgctg cggtgctaag gcttatttga ttgatggccc ccaggattta caggcccatt
     1621 ggtttgcaca ggccaatacg gtgggcatta cggccggcgc ctctgcaccg gaggttttgg
     1681 tgcagagtgt tattcacggc ctgaagcaat acggcgccca aatgcctgaa gaattggccg
     1741 ggcgggtgga aaacgtcagt ttttcactgc ccaaagaatt gcgttagggc ggcttttgcg
     1801 ccgcttgcgt acaggcatct gaagggctga ttaaaaatcc ctgtggcctc tgtgcacata
     1861 atctcgccag caggcttctt ttattcgaaa acttgatgta taacaggcgc ttaagataag
     1921 tttacccggt gcgcacagat tcttcggcgg gctctgtgag cggtagcaac aaatctttaa
     1981 tgggatgcgg cgcaatgtct attaccaagt cagagctgat tgatcgtatt gctacagcac
     2041 aggaccaact ctcgcccaag gatgttgaga ttgcggtcaa gttactactc gaatttatga
     2101 gccaatccct cgccaagggc gagcgcattg aaattcgcgg ttttggcagt tttgccctgc
     2161 attaccgcga accccgcgta gggcgcaacc ccaagaccgg tgaggccgtt gccttagaag
     2221 gcaaatacgt tccccacttt aaacccggta aagaaatgcg cgatcgggtc aatgaaagta
     2281 tcaaacagaa aaaacgccgc cgctaatatc tcctccaccc atcgctttcg gcaagtgtat
     2341 tacgagggct ttgccctcag accattcgca gccatggtat ttatttttcc ctcagttatt
     2401 ctttgtcagt tttgaataat tgcacgcgca gtttcgaagg gttcgtctaa tctgcgagtt
     2461 agtcagccta tttaaccaaa gagcgaaaaa agcttatagc gtcgatgctc acctgacggg
     2521 tggttctcct gtctgcattt ttcattatat tgagtgttga ttgattttat gtatgaatcc
     2581 aagcggcaaa atcccattac tcaagcaaaa tttatccgtc gattgtttcg gcaattggct
     2641 tgggtgttta ttctcttggc cttttcgctg ttaattggaa tgagtggtta tgtgcggttt
     2701 gagggcttaa gttgggttga tgcattttta aatgcctcta tgttgttggg cggaatgggg
     2761 ccggtaaatc cgccgcaatc atttgccgga aaattattcg caggcttctt tgcgctttat
     2821 gcggggttgg tgtttattgt ggctgcggcc ttgttgttta caccgctttt gcaccgtctg
     2881 ttacatcatt tccattggga tcaagaatag tctacgattc ccggtatttt tgtgtttggg
     2941 taatctgcgc ttctggtaag cttgggctag ccagctaagg ctgggttgca cactaaattt
     3001 atggtttttg gggtggtgtt tcccctctgt ttgattggat gatgtttatg aaaggtctta
     3061 agttgctagg tatttctttg attgtggcag ggggttgggc cctggcttat ggtggtttta
     3121 gttataccca agataacact gcaatcaaac ttggtcccct tgagcttgcc gtaaaagaaa
     3181 agaaaaccgt agatataccc ctatgggtgg gggttggggc gcttttgggt gggggtttat
     3241 gcctggtggt gggttttcgc agccgtccgt aattgcggtg gtttgggctg attttttgat
     3301 aatttcttgg gttggaagac gaggtgagtg atatgaaaat tattgcattt gcagcgagca
     3361 gcagtcggca gtcgattaat aaacaattga tcacctatgc ggctggtttg ctgcagggcg
     3421 ttgaggttga aatactcgat cttaatgatt acgaattgcc attatttagt gttgaccgcg
     3481 aggcgcaatt gggccatccg caattggcga aggatttttt ggccaaaata gccgccagcc
     3541 aaggcttaat tatttccttt gcggagcata acagcaccta tacagcggcc tataaaaatc
     3601 tttttgattg gtgttcgcgc cttgagaaaa aagtctttgc ggataaaccc gctgtctttc
     3661 tggctacttc accgggtggg cgcggtggtg ccagtgtgtt aagcgaagca ctcgcgtctg
     3721 ctgggcgctt tggtgcccag gtggtggcga gcctagcggt gcctaatttt gccgataatt
     3781 ttgatgccgc ggccggtgtg ttaactcacg cggagttaaa tcagcagttg gtggctgccg
     3841 tgggggcatt gcaggctgct agaggttaag gcgcagattt ttggctattt ctcggcttag
     3901 cgttgccggt acttcgtgtt cgcgggcgag gctcggccag tttgccacct gttgggtaat
     3961 ctcatcgatg attgtattta ttttggcgcg gccaaacagt gggcttaagg attccaggct
     4021 gtaaaaatct tgccgggtga agttgtcccg tttgccattg aggctcatcc aatggctatt
     4081 gacccagggg ctgcccggct tatagctgta ggctaagtcg taggcggggg ctagttgcca
     4141 ggttttatcg cgcaaaataa aagcactatt tttggaatga tcatcgtggt tgcgggcaat
     4201 tatattaaat gccatgcgcc taaatagttg ttcggcatcc aaagccgtta aacgcagttg
     4261 gcgggccacg gcaaaaattt ccgcatacga aaatgacccc ggcattttgt aattgacgtg
     4321 tgctaagcca ttgagggttt gtatatgcag tttttcattg ccgatgcgat caaaacgctg
     4381 ggtgatgaaa tggcggcgct tgccctctgg caatagagag cagggcatca tgtggatgcc
     4441 gcagctagtg gccattaaat aataaacata ctccatggcc ccataaccca ttgggtcgcc
     4501 aaaggtttcg ttgttttgat tgtgttcccg tacaccatca aatttcatta aataatgagt
     4561 aaaccccgca ggtgctttgg tttggcctga gcgcacttgg gtgaagtctt tattaaatgc
     4621 cagtactgct ttagggcggg cgccgccggc actcatgcct acggataaaa gtgccagcat
     4681 agcctcgcgg tcttcttggc cctgtttgtt taattccaat ttaaaattgg cgcggctatc
     4741 aagcacttct tgggcgatag tgactaggga ttcaatggct acgggctggg aggcgttgag
     4801 gttttttagg gtggtggctg gagcatattc caaagcgccc atgccgcgtt tacccgtgta
     4861 ttttaggcgc tgcaaggggg ttatttgctc cgcttgttta cccttgctcg ccatccaggc
     4921 gtttaatacc gcgttgccga agtcatctgg cagcgagtcg gctaacaggc caggcagccc
     4981 cttaaatgtg gcgtgatcca gttcaggaaa ggtatagatg cgtttggcca agggcatttt
     5041 caggggggag agttctatgc ccgtgtcgag aaattccgcc gtatattcaa aagcacccag
     5101 gccagtttgg gtatcgaaac tcgccgcacc gacggtttta cctaggtaat taacgctaat
     5161 aacatctagg gacatgagtc agctaccagt cagaggtggg tgtcgggtgg gctattggat
     5221 tgcgctcggc gcgggatgcg cgcttacgtt gtttggcttg taatttggcc agttgcacgg
     5281 gggagatggt ttgggggggg aggaatttgt ctaagtgctc ggcaagatta agtgcgagta
     5341 tgagtgcgac aaaagcctct agctgcgctt tgcctttttc agcgtttaac accaccttgc
     5401 gggtaactcc ggcaagctct gcaagccttg cttgagtcag atcggcattc aggcgagctt
     5461 gtttgagtcg ctcaccgagc gcttctgcaa cggcggcagg gggcatggat tgactgatca
     5521 taatgttacc aaaatggaat tttaaccgtt aaatctatct taatgtaacc ataatagcat
     5581 cttatgagaa ggtaattgga ttctctgtcg attgagttcc tattttggat cattaagtga
     5641 tttatttgcc tttaatgtgc taaaaattgg atattgtatc gggtggtatt agattgggtt
     5701 tggcgctcgg taccatttgc agggactggg cgtaattggc tggctgaagc ccggggcgcg
     5761 agagactgtt gtgtggagcc aaagcgcagc gagtggctag cgcgataggt ttgcaggtgt
     5821 tgcggggctt gccactattg tttgcttaca aatcggggga agtttatgca cacctacgtg
     5881 gcatttacat cagacatgtt tcaaccgttt ttacccgaat ccgcgcaggt taatccccat
     5941 tgttacggcg cagaattggc gtggtggctc tgccagcaat tagccgggaa ggggtttgtt
     6001 accagttatc ccaacagtga agattggggc tggtttattg aatacgctgt ggatgactat
     6061 gagtattggt tgtgctgcgg caatgtgccg ggggcgtcca atagatggca aatttaccta
     6121 gactgcaaac ctaaaggctt gtttggcaga gacaaggctc cggttgcatt ggccgaggac
     6181 ttattaaccg cactggtaga tttattggcc actaccagtg gggtaggcga attgatttgg
     6241 cgtgacagct ctgagtagat atctctccat cgatttacca gcctgttaaa ttgcgcttat
     6301 gaattgagga tgctttgatc actgagcgag tacaaatgag tattgctatt ttgactcgca
     6361 cctgaaagaa acctaaaaac gaatttggtc tgcgcatttt tgatgcaagg caatcaattc
     6421 ttgcggcgcg ctctggggga aagtggtgag gcgcctgaag cggttgcagc aacaagcggg
     6481 gtgagccaac tccaagcatc gttgctgaaa atgcaccaat cttggtctag gccacatttg
     6541 gctgaggatt tttcagcaaa catctgtgat tttttacccg caaaaatttg cacgcggtca
     6601 ctgagcctag cgtacacatg atctcgcccc ttctttcaaa gcgtaaaaca acaaaccagt
     6661 cacacggtgt gtagttgtcc gtgaacttgg cgagtccttt gctactctgt aaacaaaccg
     6721 tcacacgcta acggcagtat tgcggtgagg gtaggtgagg tttaaccacc tcaaacaaca
     6781 cgtacttagc agtagatact gaaaaccgag gaccaaagga gcctaaaact atgcaagatt
     6841 atattgaaga actattagaa attacctgct ccgtcaacga tgaagacgag caatttagtt
     6901 ttgatgatta ttcggaagtc taaagccatt ttgctgcccc ctcacagagt gagtggccaa
     6961 gaggcaataa acgatagttc agcctatcgt tgcgcgtgat cgttaatctg atccggtgtg
     7021 tgatcaaatg tgatctaaac agtgacaaaa atagtcccgc gcgacaaagc ccaagcgttc
     7081 aggtgtcgag cccaagctta ggcctgtagg taaaagccct gcgcatataa caatcgcgtg
     7141 tgcgcagggc ggccagtaaa actgtgaatt gatttatatt taccgcttta taggccagag
     7201 ttagaaggat aaatgctaag gaagcgtact atagttagtg cgcagcgtgc cctaggggct
     7261 gaagttggcg cgctgtttag gctatagtgc cgctttgagt gctggtaatc ccctgctgca
     7321 cttgtcccta gatgtggaat cgcgattatc ctatgttgtt gctctcgact gaaaaagcac
     7381 gcgcggtata ttttttcgat caggccgaaa tcgtcggcca acttctcctc cctgaattcg
     7441 aatccatgtt agatggtttt gtgccgtcta aagaatgggc tttgcgcacc atgcaggcgg
     7501 tgtacgttga gattaaccat cgcctgctta ttaccgctgc agtatttttt aaagtggggt
     7561 ttgatgatca cggttggatc gacggctcat ggaacatgcc cctagcggat cttgccaagc
     7621 gcggccaagc cggcccagac cttggcgcag gccccatcca catggccacc ttttcccaat
     7681 gccccatcga cctatacaaa cagctgttgt gggacccgga agtaaagggg cgtcaaaacc
     7741 acctaggaca attaaaaaag gcggtatcgc gcaatcgttt agggatttgt tttgttgacg
     7801 atggccagga tgaccccaat aaaccggata atttggtggc ggctatgggg gagcggctgc
     7861 aaacggtgct gcatcagcat ttgaatcaac gggatgacac gggtattaaa aatgtcctgg
     7921 cgaaagtaat tgacgagcag gcgcgcattc ccaccctggt acccagtagc gaagatttaa
     7981 aagagcgttt ggaattactt gagcaaatgg tgcaggagcg ggatgagcag ctggcgcatt
     8041 tagagcgcca actggccgag gcccagcaac aattagtgca ggccactgag cacagccaga
     8101 cgctgagcaa taaagctgaa gggctgcgca attattatga gcacaagatc attcgcatgg
     8161 agcagcaaca attaagcctt gccgcacaag cccaattaag cgcggccagt gatagcgaaa
     8221 tagcggcggc ggtgcatgaa gccacgcagg attttgccga actgttgcag cgcagagaaa
     8281 tagaactctt ataccgcagc gaacgagagg agcaattgct caaccagttg cgcgaacaaa
     8341 atgaacatcc cagtgcagcc gacgagcgta tttttgatta tttgcaaatg ttaaatgacc
     8401 acggcgtgcg ttttgtacat taccgcgcag gccttacccc gatacaaatt ccggtggggg
     8461 aattggcgga atttttaaaa tctgccacgg cctatacagc gcaaaaattg ggtgtaacag
     8521 aagccttata taacgcgtgg ctgagccatt ttttaatgcc agtgtgcagc gccgcaaccg
     8581 tcaatggccc ctgtgcggcg agtatttatc gcgttgaaga accgcgccat tttattcctg
     8641 gcgtgagcga ttgttgcact gaacatcagc ctaaaacgtg atttttattc ctattgtggc
     8701 aacaggcagt taaaacccat cgccggttaa ttagctgccg cccgcccaga ccatgtattt
     8761 atcttccccc tccccacttt ataaccttgc acaacgggcg caggtacagc cagttgcctg
     8821 gtcgtgcgcg cgggcggcca attgccgcat ctggattaag cgcgatgatc tattacatgc
     8881 agccgtcagt ggcaataaat tatttaaatt atttcatcac ttaatgcagg caaaaaaatg
     8941 tgctgcacga ggtgtaatta gctttggcgg cgcctattca aatcacctct acgctctggc
     9001 gcgagtggcc aaagcgctta atctgccagc cgttgcgatc attcgcgcag gcgatgcgga
     9061 tctaaacaat ccgaccctca gcgaactgcg cgcccagggt atgcagttgt tgccgatcag
     9121 ccgagagcaa taccgccgca aaatggagcc cgcatttagt gcggaattaa caacgcgcta
     9181 tgcggatttt tatctaattc ccgaaggtgg ggcaggcgtg ttgggggctc agggttttgc
     9241 cgattatgca gaggcggtat cggcccaatt gagggcacag gacataaccc cggatagcgt
     9301 ttggctgcct gcgggcactg gcaccagcgt agccggtttg gcgtcccggt ggcacaatgt
     9361 gcaggcagtt ggcgccctgg ccataaccga tcctaagccc tacacccagg cagttgaagc
     9421 ctggacgcag cagttaactg gctctgccgg tgtacacagg gtgaactggc acggttttga
     9481 taggcccttc ggcaaaatac ccgcatctat actggatgtg tgccgagaat tttggtgtga
     9541 aaccggtgtg ttgtttgacc ccatttatgg cggcaagggg gtgttagccc tgcaaaaagc
     9601 ctttgctcag g
//
LOCUS       DAOQGS010000094         9566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3349_length_9566_cov_5.019031, whole genome shotgun sequence.
ACCESSION   DAOQGS010000094 DAOQGS010000000
VERSION     DAOQGS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_9566_cov_5.019031"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..595)
                     /locus_tag="PKZ52_05205"
     CDS             complement(59..595)
                     /locus_tag="PKZ52_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HNG59203.1"
                     /translation="MSSLQDQLLKAGLIDSKKAKTIQKEQRKQTKVQHKSKEKIPDPI
                     KAAAEEARLEKQQKDQALNAQRNEEAHKKAIVAQIKQLIELHKLPRSGEIAYNFTDGK
                     TIKKILINAALQKALLNGQLAIAKLGERYELVPYPIANKIIERDSSFIISQAQLGLSA
                     AAGDDPYADYQIPDDLMW"
     gene            complement(608..1324)
                     /locus_tag="PKZ52_05210"
     CDS             complement(608..1324)
                     /locus_tag="PKZ52_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNG59204.1"
                     /translation="MSTPSVIASLTTFLEDFTGRFADVFEPHRALGAIVGGLSAPVEL
                     GQDELLELVLNQATDSAAAVLANAQRAAQWAAIVDGVEDMMNAGPWLLEQYSPLTTPP
                     GAEVAQWCDGYLQAYWYCEDVWQEFYAVWQELAAESDDEDLNNVAEFHPVLVNMLFLI
                     AHWDSALEEMENLAELPENINELFAEIAEGVTHFFQLMREMAASIEGDKTPYTKPATP
                     NRNDLCPCGSGKKFKKCCLN"
     gene            1705..1911
                     /locus_tag="PKZ52_05215"
     CDS             1705..1911
                     /locus_tag="PKZ52_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNG59205.1"
                     /translation="MSTMTGTVKWFNESKGFGFISPESGPDVFAHFSAIQGDGFKTLA
                     EGQKVRFTVTEGKKGPQAENITKI"
     gene            2113..2505
                     /locus_tag="PKZ52_05220"
     CDS             2113..2505
                     /locus_tag="PKZ52_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNG59206.1"
                     /translation="MGAIAMHSIEELTLYLREFARARDWEQFHNPKNLCMALSIEVAE
                     ACEHFQWLTPEQAQNLNSNQKEAVALELADIQMYLVRLADQLGIDIWQAINTKTQLNE
                     AKYPAALVRGNAAKYTAYQQDLTSNKTS"
     gene            2519..3196
                     /locus_tag="PKZ52_05225"
     CDS             2519..3196
                     /locus_tag="PKZ52_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNG59207.1"
                     /translation="MWLVTDLAGNECLPWLPRRIFCIGRNYADHAREMGHNPDLEPPF
                     FFTKPLTSLCPASGNWQLPDFSRQVEHEVELVIGLRVPPEQGRNISAEGAQSWIAAAG
                     LGIDMTCRDLQREAKQAGRPWDIAKGFDAAAPLTPLVQLDAAGLNTLGAMQLKVNGEL
                     RQTGHWQEMLWPPGELIAHIARYFALQTGDLIFTGTPAGVGPLKPGDHLTATLAGLPH
                     SVDLRIY"
     gene            3295..5316
                     /gene="rep"
                     /locus_tag="PKZ52_05230"
     CDS             3295..5316
                     /gene="rep"
                     /locus_tag="PKZ52_05230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HNG59208.1"
                     /translation="MSKLNPRQHEAVRYIDGPCLVLAGAGSGKTSVITRKIAYLIEDC
                     GLSARHIAAVTFTNKAAREMKERVGKLVKRKDAQGLTVSTFHNLGLNIIRREYKALGY
                     KPGFSILDTEDARSILKELMLRDGDLNTEILDAVQNQISNWKSALTTPQHALEKAENA
                     REQTYALVYERYLQALQAYNAVDFDDLIRLPAHLFLGNEEVLQRWQQRIRYLLVDEYQ
                     DTNSSQYMLVQKLIGARGALTVVGDDDQSIYAWRGARPENLSQLKQDFPNLHVIKLEQ
                     NYRSTARILKVANQVISNNPHEFNKTLWSDLGVGEMLRVIRCPTEDAEAERVANEILS
                     QKLRRQAQFRDFAILYRGNHQSRLIELKLQQYQIPYNISGGTSFFAKTEIKDIMAYLR
                     LLVNPADDNAFLRVINTPRRQIGTNTLEALGRYATEREISLLDACQEVGIQTLITGKS
                     LDRVRHFADWLQKVRENCHNHDPVAAIREMVEDIDYESWLHQNASSPKVAEKRLENVW
                     YLVDSIKNQLERNEDDSKTHEENLNAAIAKLLLLDLLERQEEEDTSDRVQMMTLHASK
                     GLEFPHVFLVGLEEELLPHRNSIDSGNIEEERRLMYVGITRAQRTLTLTLAGTRKQFG
                     EKQETTPSRFLDEMPPEDVEKEGFGNATPEQVEKKGKETISNIMRLFDE"
     gene            5335..6231
                     /locus_tag="PKZ52_05235"
     CDS             5335..6231
                     /locus_tag="PKZ52_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNG59209.1"
                     /translation="MLNELFAVMAPTLVGTLIGWIWAKKSVPFPADFVSKIVMYIGTP
                     CLFMSVMSQVHVSVDEVSKMAGAFALGLGFMALVSVAIIRLMGLEMRAYLPPLIFPNN
                     GNMGLPICLFAIGQEGLALALAPFMVMVLAQFTLGLFIVAPNDGSVWRRCRDILVHPI
                     IISMGISVLLLTQYLTLPRWISNTVDVMGGFAIPLMMITLGVSLARFRLTLWFKTLFF
                     SVLRVVGGAIIGIVICQWLNITGNEKIVITLQMAMPMAVMNYVIALEYDRKADEVAAM
                     VLMSTLVGFLVLPILIYWLKLT"
     gene            complement(6276..6773)
                     /gene="tpx"
                     /locus_tag="PKZ52_05240"
     CDS             complement(6276..6773)
                     /gene="tpx"
                     /locus_tag="PKZ52_05240"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNG59210.1"
                     /translation="MATVTLKGNPFSTVGELPAVGSQAPNFTLVQADLSEITLASLSG
                     KKVLLNIFPSIDTPTCALSVRTFNESAANLANTQVLCVSADLPFAGKRFCGAEGIERV
                     ATASSFRSDFGRSYGVAFANGPLAGLLSRSVVVIDEQGKVLYTEQVAETADEPNYAAA
                     LAALG"
     gene            6980..8131
                     /gene="argE"
                     /locus_tag="PKZ52_05245"
     CDS             6980..8131
                     /gene="argE"
                     /locus_tag="PKZ52_05245"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HNG59211.1"
                     /translation="MASQSTRYIQRLAQLVKLPSVSSTDARWDMSNRAVVELLAQWLE
                     PLGFNCQIHEIANAVGKFNLIAQRGSGPGGLVLAGHTDTVPCNPERWAQDPFTLSERD
                     GRLYGLGATDMKGFFPCILAAVEQLQHSELKQPLIILATADEESTMAGARALQQQGLP
                     AARAAIIGEPTGLTPIRMHKGILLHAVEVIGQAGHSSNPNLGTSALEAMHSVTSELMA
                     FRAELQHKYNHPSFSFPKPSLNLGCIHGGDNPNRICGRCELLFDLRPLPGMDSQALKD
                     ELKQRLIPIAARTHTQINLKELSAGIEPFEEGAESELVKQVETWSGHHSAAVNFATEA
                     PFLQQLGMQTVVCGPGYIDQAHQPDEYIPLEHIPLTVDFLLKAIGHYCL"
     gene            complement(8270..8605)
                     /gene="nirD"
                     /locus_tag="PKZ52_05250"
     CDS             complement(8270..8605)
                     /gene="nirD"
                     /locus_tag="PKZ52_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNG59212.1"
                     /translation="MNTTVQNHWQAVCKAEDIGNCLGVRALIEGQQVAIFNVNNEFYA
                     LDAIDPFSNAAVLSRGIVGDLKGQLVVASPVYKQHFNLRTGECLEDPSVKLATYAVRN
                     NQGVIELAV"
     gene            complement(8620..>9566)
                     /locus_tag="PKZ52_05255"
     CDS             complement(8620..>9566)
                     /locus_tag="PKZ52_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
                     /protein_id="HNG59213.1"
                     /translation="WNELIMQDKLAPLQDTNDHFMANLQKNGTYSIVPRIAGGEITPD
                     KLIVLGQVAKKYNLYTKITGGQRIDLFGAQQHELPLIWRELVDAGFETGHAYGKSLRT
                     VKSCVGSTWCRYGVLDSVGMAIRLEDRYKGLRSPHKLKFAVSGCTRECAEAQSKDVGV
                     IATENGWSLYVCGNGGMKPRHADLFATGLSDEQLVKYIDRFLMFYVRTADRLQRTSVW
                     MDNLEGGLNYLKSVILDDKLGLGAELEQEMQAVVNTYQCEWKSTIEDAEKIKRFRTFV
                     NSDAADNTVVFVEEREQIRPATEEELVRFVSSNELEPA"
BASE COUNT         2422 a         2485 c         2327 g         2332 t
ORIGIN      
        1 ccatttacgg cggcaaccgg gccgcctgat agtgtctgcc acgggttttt aacaaggttt
       61 accacattaa atcatcggga atttgataat ccgcataagg gtcatcacct gctgcagcgc
      121 ttaaacccag ttgggcctgg ctaataataa aactgctgtc gcgctcgatt attttattgg
      181 caatcgggta tggcaccagc tcgtagcgct cacccagctt cgctatagcc aattggccat
      241 ttaacagcgc tttttgcaaa gccgcattaa tgagaatttt tttgatggtt ttaccgtcgg
      301 taaaattgta ggcaatctca ccgctgcgcg gcagtttatg cagttcaatt aattgtttga
      361 tctgcgccac tatggctttt ttgtgggcct cttcattgcg ctgggcgttt agagcctggt
      421 ctttctgttg cttctccaaa cgcgcttctt cagcagctgc tttaataggg tcagggattt
      481 tttccttgga tttatgctgt actttggttt gcttgcgctg ttccttttga atggttttgg
      541 cttttttact atcaattaag cctgctttga gcagttgatc ttgcagggaa ctcatctgtg
      601 ccttccatta atttaaacaa cattttttaa actttttgcc gctgccgcag gggcatagat
      661 cattgcggtt gggtgtggca ggttttgtat agggcgtttt atcgccttca atactggcgg
      721 ccatttcgcg cattaactgg aaaaaatgcg taacgccttc ggcgatttcg gcaaataact
      781 cattgatatt ttccggcagc tccgccagat tctccatttc ctcaagggcg ctatcccaat
      841 gggcaattaa aaacagcata ttcaccaaca ccgggtgaaa ttccgccaca ttattcaaat
      901 cctcatcgtc agactcagcc gctaactcct gccagaccgc ataaaattcc tgccacacat
      961 cttcgcagta ccaataggcc tgcaaatagc cgtcacacca ctgggctact tcagcaccgg
     1021 gcggggtggt aagcgggctg tattgctcca acagccaagg gcctgcattc atcatgtcct
     1081 ccaccccgtc aacaatggcc gcccactggg cagcccgctg ggcattagct aaaacagctg
     1141 ccgcactgtc ggttgcctgg ttgagcacca attccagcaa ctcatcctgg cccagttcaa
     1201 ccggcgcact cagcccgccg acgatggcac ccagcgcccg gtggggctca aatacatcgg
     1261 caaaacggcc ggtaaaatcc tctaaaaaag ttgttaggga ggcgataaca gaaggggtag
     1321 acataggaga ccacgcagct aaaagctcaa gcatacctca agtggctgag cagggcagcg
     1381 cagccattgg gtgcaggtag caaatctgtg tgacatttgt gtgcaatacg cggaatttcc
     1441 cgcataaata gccgtatttt tttgcctagc tgaacggtta gtgaaactta gcgccagttc
     1501 aacaaataaa gattgactaa acgggcaaaa ggtttaacct agcctcgaag ctagaaggtg
     1561 cggctttttt tgtgtttacc ggtacgatgt tcgatttgta gtaactcgtc ttgtttcctc
     1621 acgcaatccc tctctttggc tcaaccctgg catcaaacac caggtgacga aacttcaaat
     1681 tcttacattt ttaaggtagt acatatgtcc acaatgaccg gcactgttaa atggtttaac
     1741 gaaagcaaag gttttggttt tatttctcca gaatctggcc ctgatgtgtt tgcccacttt
     1801 agcgcaatcc aaggcgacgg cttcaagact ttagcagaag gccaaaaagt tcgttttacc
     1861 gttactgaag gtaaaaaagg cccacaggcc gagaacatca ccaaaatcta atttaaaccc
     1921 tttcggccag gtgttaaaac atctggctga tggagttaaa gcgggcctta tagagcaatc
     1981 cttaaagcct gcgccttggt aagcgttaac ctgttaaaac cgaggcctgt aatttagtag
     2041 ttggattata aaaagggtaa ggcctaggct ttaccctttt ttgttgtctg catgtttcac
     2101 tcggcgccag ttgtgggagc aatcgcgatg cactccattg aagaattaac actctatctg
     2161 cgtgaattcg ccagggcccg cgactgggag caatttcata accccaaaaa cctatgcatg
     2221 gccttaagta ttgaagtggc cgaagcctgt gaacatttcc agtggctcac cccggagcaa
     2281 gcgcaaaacc tcaattccaa tcaaaaagag gcggtggcat tggagctggc ggatattcaa
     2341 atgtatttgg tgcgcctggc ggatcagctg ggcattgata tatggcaggc catcaatact
     2401 aaaactcaac tgaatgaagc taaatacccc gccgcattgg tgcgcggcaa cgcggctaaa
     2461 tacactgcct atcagcagga tctcacctca aataaaacct cttaatttag ggcctaatat
     2521 gtggttagtt accgatttgg caggcaatga atgcctgccc tggctgcccc gacgcatttt
     2581 ttgcatcggc cgcaattacg ccgatcacgc gcgggaaatg ggccataacc ccgatctaga
     2641 accgcctttt ttcttcacca aacccctcac cagcctgtgc cctgccagcg gcaattggca
     2701 gctgccggat ttctcgcgcc aggttgaaca cgaggttgag ctggtgatcg gccttagggt
     2761 gccgcccgag caagggcgca acattagtgc tgagggcgcc caaagctgga ttgccgctgc
     2821 gggcctgggt atcgatatga cctgtcggga tctgcagcgg gaggctaaac aggctggccg
     2881 cccgtgggat atcgccaaag gctttgacgc cgccgctcca ctcacccccc ttgtgcaatt
     2941 ggacgctgcc gggcttaaca cactgggcgc catgcagtta aaggttaatg gcgaactgcg
     3001 ccaaacgggc cactggcagg aaatgctctg gccgccgggt gaattgatcg cgcatattgc
     3061 ccgctatttt gccttgcaaa ctggcgatct tatttttacc ggcaccccgg caggcgtcgg
     3121 cccacttaaa cctggcgacc acctcaccgc cactctggct ggcctgcccc acagcgtgga
     3181 tttaaggatt tactaaaacc cgctgggaac cggcaaaaac accggcaacc gcgacaatcc
     3241 cccacaacta aatgggcata aggcgtaaaa taactctttt gccctgaggt gttcgtgtcc
     3301 aagttaaacc cccgccaaca tgaagccgtg cgctacatcg atggcccttg cctggtattg
     3361 gcaggggcgg gcagcggtaa aaccagcgtg atcacgcgca aaattgccta cttaattgaa
     3421 gactgcggcc tgagcgcccg ccatattgcc gcagttacct ttaccaacaa agccgcccgg
     3481 gaaatgaaag aacgggtggg taaattggtc aagcgcaaag acgcccaggg ccttacggtt
     3541 tccaccttcc acaatttggg cctgaatatt atccgccggg aatataaagc cctcggctat
     3601 aaaccggggt tttcaatatt agataccgag gacgcccgca gtattttaaa agagctgatg
     3661 ctgcgggacg gggatttaaa tacggaaata ctcgacgcag tacaaaacca aatttccaac
     3721 tggaaatccg ccctcaccac cccgcagcac gcacttgaaa aggccgaaaa tgcccgcgag
     3781 caaacctatg cactggtgta cgagcgttat ttacaggcgc tgcaggccta caacgccgta
     3841 gattttgacg atttaatccg cctgcccgcg cacttatttt taggcaatga agaagtttta
     3901 cagcgctggc agcagcgtat tcgttattta ttggtggatg aataccaaga caccaactcc
     3961 agccaatata tgctggtgca aaaactaatc ggcgcgcgcg gcgccttaac tgtggtgggc
     4021 gatgacgatc aaagtattta cgcctggcgc ggcgcccggc cggaaaacct cagccaatta
     4081 aaacaggatt tccctaattt acacgtcatc aaactggagc aaaattaccg ctccaccgcg
     4141 cgcattttaa aagttgccaa ccaggtgatc agcaacaacc cccacgagtt taataaaacc
     4201 ctctggagtg atttaggcgt gggtgaaatg ctgcgggtca ttcgctgccc cacggaggat
     4261 gccgaggccg agcgggtggc taatgaaatt ctcagccaaa aactcaggcg tcaggcgcaa
     4321 tttcgcgatt tcgcgatttt atatcgcggc aaccatcaaa gccgtttaat tgaacttaaa
     4381 ttgcagcagt atcaaatacc ctacaacatc agcggtggca ccagtttttt tgccaaaact
     4441 gaaattaaag acatcatggc gtacttgcgt ttattggtga atcctgccga cgacaacgcc
     4501 tttttacgcg ttattaatac cccccgccgc caaattggca ccaacaccct ggaggcctta
     4561 ggccgctacg caacggagcg ggaaattagc ctcttagatg cctgccaaga agtgggcatc
     4621 caaacgctga ttacgggcaa aagccttgat cgcgtgcgcc acttcgctga ttggctgcaa
     4681 aaagtgcgcg aaaactgcca caaccatgat cccgtggcgg ctatccgcga aatggtggaa
     4741 gacatagatt acgaatcctg gctgcatcaa aatgccagct cgccgaaagt agcggaaaaa
     4801 cgcttagaaa atgtttggta tttagtggat tcgattaaaa atcaattgga gcgcaatgaa
     4861 gacgatagca aaacccacga agaaaattta aacgcggcca tcgcaaaatt attgttgctg
     4921 gatttattgg agcgccaaga ggaagaggac accagcgatc gggtgcaaat gatgaccctg
     4981 cacgcctcta agggcttgga atttccccat gtgtttttag tagggctaga agaggaatta
     5041 ttgccccacc gcaactccat tgacagcggc aatattgagg aagagcgcag gcttatgtat
     5101 gtgggcatta cccgcgcaca gcgcacactc actctgacgc tggccggtac gcgcaaacaa
     5161 tttggcgaaa aacaagaaac tactcccagt cgatttctcg acgaaatgcc ccctgaagat
     5221 gtggaaaaag agggctttgg caatgctacg cctgagcaag tggagaaaaa aggcaaggaa
     5281 actatttcca acatcatgcg cttattcgat gaataatccc ggactcatcc ctctatgcta
     5341 aatgaattat ttgccgttat ggcgcccacc ttggtcggca ccttaatcgg ctggatttgg
     5401 gcgaaaaaat cagtgccgtt tccagcggac ttcgtctcta aaattgttat gtacataggc
     5461 acaccctgcc tgtttatgtc ggtgatgagc caagtccatg tcagcgtgga cgaggtgagc
     5521 aaaatggccg gcgccttcgc cctggggctc ggctttatgg cgctggtgag cgtggcgatt
     5581 attcgcttga tgggcctgga aatgcgcgct tatctgccgc cgctgatatt ccccaataat
     5641 ggcaatatgg gtttgcccat ttgtttattt gcgataggcc aagagggcct ggctctggct
     5701 ttagcgcctt ttatggtgat ggtactggcg caatttaccc tcgggctatt tattgtggcg
     5761 ccgaacgacg gcagcgtatg gcgccgctgc agggatattc ttgtccaccc cattattatt
     5821 tccatgggca ttagcgtgtt gttattgacc caatatttaa ccttgccgcg ctggatcagc
     5881 aataccgtag atgtcatggg cggttttgcc atacccttga tgatgattac actgggtgtg
     5941 tcactggcgc gttttcgcct gacgctatgg tttaaaactc tattttttag cgttctgcgc
     6001 gtcgtgggcg gcgcgattat tggaattgta atttgccagt ggctcaatat taccggcaat
     6061 gaaaaaattg taattaccct gcaaatggcc atgcccatgg cagtaatgaa ttatgttatc
     6121 gccctagaat atgaccgcaa agccgacgaa gtggcagcga tggtgttaat gtccaccctc
     6181 gtcggctttt tggttttacc tattttaatt tattggttga aactgacgta aaaaacacag
     6241 cgcccaacag caccaacctg cgggctttag tgcgcttaac ccaaggctgc cagggcggca
     6301 gcgtaattgg gttcgtcggc ggtttcggcc acttgttcgg tgtacaacac cttaccctgc
     6361 tcatcaatca ccactaccga gcggcttaac agtcccgcca agggcccatt cgcaaatgct
     6421 acgccgtagc tgcggccaaa atcggagcga aagctggatg cggtggcaac gcgctctata
     6481 ccctcagcgc cgcaaaaacg tttaccggca aagggcaaat cggcggagac acacaacact
     6541 tgggtattag ctaaattggc cgcactttca ttaaaggtgc gcacagacaa ggcgcaggtg
     6601 ggtgtatcaa tggatgggaa aatattcagc agaacttttt ttccgctgag gcttgccagg
     6661 gtaatttcgg ataaatccgc ctgcaccaag gtaaaattgg gcgcttgact gcccacggca
     6721 ggtaattcgc ccacggttga aaatggattg ccttttaatg ttacagttgc cataaaaacc
     6781 cctatagagt gttgggaatt aattgaacgg cgtgcaaaat gccgggcccg ataataccgg
     6841 gaatatccca gccgtcctac gcttttctga aatagcgcgg cagaattctt gtgccctgct
     6901 cgcacgtggg gtttaaactg tgcggccatt aaccgctata ctcagcgcaa ttccacgccc
     6961 ataccccaag gcaattttta tggcctcgca atcgacccgc tatattcaac gtctggcgca
     7021 attagtgaaa ttaccttccg taagttccac ggatgcacgc tgggatatga gtaatcgcgc
     7081 cgtggtggaa ttattagcgc aatggttaga gccgttagga tttaactgcc agattcacga
     7141 gattgccaat gcggttggga aatttaattt aatcgcccag cgcggcagcg gccccggcgg
     7201 tttagtgctg gcgggccaca cggatacagt gccgtgcaac cctgagcgct gggcgcagga
     7261 tccatttacc ctcagcgagc gcgacgggcg cctgtatggc ctgggcgcta ccgatatgaa
     7321 agggtttttc ccctgcattc tcgccgcagt ggaacagctg cagcacagcg agttaaaaca
     7381 gccgttaatt attttggcta ccgccgatga agagtccacc atggctggtg cccgcgcgct
     7441 gcagcaacag gggttacccg ccgcgcgcgc cgccattatt ggcgaaccca caggccttac
     7501 acccattcgt atgcacaagg gcattttatt acacgccgtt gaagtgatcg gccaggccgg
     7561 gcattcttct aatcctaatt taggcaccag cgcccttgaa gccatgcaca gtgttaccag
     7621 cgaattaatg gcctttcgcg ccgaattgca acacaaatat aatcacccaa gtttttcttt
     7681 tccaaaaccc agtttaaatt tgggctgtat ccacggcggc gataacccca accgcatctg
     7741 cgggcgctgc gaattattat ttgatttgcg ccccctgcct ggcatggata gccaagcgtt
     7801 aaaagacgaa ttaaaacagc gcttaattcc tatcgctgcg cgtacacaca cgcaaattaa
     7861 tctaaaagaa ttatcagcgg gtatcgaacc ctttgaagaa ggcgccgaaa gcgagttggt
     7921 aaagcaggtg gaaacctgga gcgggcatca ttcagcggca gtgaatttcg ccacggaggc
     7981 acctttttta cagcagttag gcatgcaaac cgtcgtgtgc gggccggggt atatagatca
     8041 ggcccatcaa ccggatgaat atattccgct agaacatatt ccgctgacgg tggatttttt
     8101 attgaaagcg atcggccatt attgccttta atgctcagcc aaaagacaaa tactaagggt
     8161 gcgaaagcac tggatacagc cgattactgc aaaataaaga tataaaaaag ggcggccgga
     8221 gccgccaaaa gctgaaagta gaggatgact ccactatcag aggaggggtc tatacagcca
     8281 attcgattac gccctggttg ttgcgcaccg cgtaggtggc caacttcaca ctgggatctt
     8341 ccaaacattc accggtacgc aaattaaaat gctgcttgta cacgggcgag gcaaccacca
     8401 actggccttt taaatcgccg actataccgc gcgataacac ggcagcatta ctaaaagggt
     8461 cgatggcatc caacgcataa aactcgttat taacattaaa aatggccact tgctgtcctt
     8521 caatcaacgc acgcacacct aaacaattgc caatgtcctc tgccttacac accgcctgcc
     8581 aatgattttg cactgttgta ttcatagtta actccacttt taggccggtt ctaattcatt
     8641 gctgctaaca aaacgcacca actcttcttc ggtggcgggg cgaatttgct cccgctcctc
     8701 gacaaacacc acggtgttat cggcggcgtc cgaattgaca aaggtgcgga agcgtttaat
     8761 tttttcagca tcttcaatag tgcttttcca ctcacactga taggtattca ccaccgcttg
     8821 catttcctgt tcaagctcgg cacccaaacc taatttatcg tccagaataa cgctctttaa
     8881 ataatttaac ccgccttcga gattatccat ccacacggat gtgcgctgca ggcgatcggc
     8941 agtgcgcaca taaaacatta aaaagcggtc gatgtatttg accaattgtt catcactcag
     9001 gcctgtggcg aataaatccg cgtggcgcgg cttcataccg ccgttgccgc acacatatag
     9061 gctccaaccg ttttcggtgg caataacacc cacatcttta ctctgggcct ccgcacactc
     9121 gcgggtgcaa ccggaaacgg caaattttaa tttatggggg gagcgcaaac ctttgtaacg
     9181 atcttccaaa cgaatggcca tacccaccga atcgagcaca ccatagcggc accaagtgct
     9241 gcccacacag gattttaccg tgcgcagcga tttgccatag gcgtggccag tttcaaaacc
     9301 ggcatccact aattcccgcc aaattaacgg taattcatgt tgttgagcac cgaataaatc
     9361 gatgcgttgg ccgccggtaa ttttggtgta cagattatat tttttcgcca cctggcccag
     9421 cactatgagt ttatctgggg taatttcgcc accggcaata cgcggcacaa tggagtaggt
     9481 gccgtttttt tgcaggttgg ccataaagtg gtcgttggta tcctgcaggg gcgcaagttt
     9541 gtcctgcata attaattcat tccaac
//
LOCUS       DAOQGS010000095         9527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3370_length_9527_cov_5.716322, whole genome shotgun sequence.
ACCESSION   DAOQGS010000095 DAOQGS010000000
VERSION     DAOQGS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3370_length_9527_cov_5.716322"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKZ52_05260"
     CDS             complement(<1..472)
                     /locus_tag="PKZ52_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG59214.1"
                     /translation="MKNNHSTLSRRIFLRGAGGITLGLPLLDAFSAKHAEAATVTDRF
                     ALFVVGANGVAMADSSRGEPEKFWPSQTGPLTYDSLRADDQQRQRTVGMFADYASRLL
                     VVRGVDQPWGSAGCDHQSGDNMCLTSAKIIGGGNSSLAGGESIDNRIAPLTVPTG"
     gene            complement(482..2914)
                     /locus_tag="PKZ52_05265"
     CDS             complement(482..2914)
                     /locus_tag="PKZ52_05265"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNG59215.1"
                     /translation="MNLTSPLRQLLLSGVCTLLLSGCGAQPAATSSSSSSSSSSSSGS
                     SSSSSSSSSSSSSSGGAAPSFRWERAFGINVGGDEVVTASGYRYEKDRDFVGGTVTST
                     VDAIANTSDDALYQTERYGNYSYQLPVAAGRYRVTLKMAEIYQTAIAARKFSVSVEGQ
                     QVVSNIDLFASFGHDVAHDVESPIVQVSDGNLSIVVSATVDNGTLAAIEVFREEKVAD
                     GENPGSVSIGKGLYTTQCETCHGASGEGSGAYPALTLPSCEACTSYKELVSKIETSMP
                     LRGALCKGSCATDVAAYILATFTEPKLIVPRIARLTHFQWKNAVQDLLYLDAPPNEQV
                     ESFSPDAFVGYDTNATQLRVSSTLRGDYRNAAEALAAQVAASTTAINRLVPQGAPTEA
                     TARARAFINDLGLRAHRRPLTTAESDRYYALFQKGPQLFPELGAFAGGVRLVVEAVLQ
                     SPYFLYRTELGQVTSNGRIPLTGYEVAAKLALAITGSGPDKLLLDAAASGALDPGKAT
                     TAVADHAERLLKTPRAQATALHLHTQTYALSRYSIIQRNPANYPQFGAGTVTSMKKSA
                     ELFFQSIFDENLGVRAILTSPNAYVDANLAPFYGLPAGFGSEFQKVDLSAQGRAGFLM
                     QLGFLTLFAGEYQPDPIHRGVFINEHVLCVNVGRPAANIPPLPEVKPGQTNRDRINAV
                     TGPGTCGAGCHATVINPLGFAFENYDPVGRFRSTDNGLPVKAADVYQNNGFEAQFANA
                     NELTAQLAESDLAHQCYAEHWLSYLYGRQAVHGDDALLTQLAAKSKSQNLSAKGVIRA
                     LVQSEYFLTREP"
     gene            3552..4892
                     /locus_tag="PKZ52_05270"
     CDS             3552..4892
                     /locus_tag="PKZ52_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNG59216.1"
                     /translation="MKIRQLIPFSLFTLLIACGGEPGTGSSSSSSSSSSSSSSSSSSN
                     STSSSSSSSSSSSSSSSSSSSSSSSSSSGVAGSLVYAIDVGGSSPMTLDGVRYEADRF
                     SSGGTVNSTTDPISGTTNSALYQTERYGTFSYSIPVSKATYSVVLNLVELYHTSAGSR
                     SFSVVVEGQTVATGLDIFSKVGHDSAYVLRANNIPVTDGALTIQLTSQVDNATLAGLA
                     IYSADGGRLEEPPAPTICPASGPCKILPLGDSITDGVGGSGGAYRVELFRQAVLNNKN
                     ITFVGSKSNGPTTVEGKSFPRSHEGNSGYSINQIANLVPSPALNGDPNIILLMIGTND
                     SWVEPNNNGAQAMAQRLGALMDKIINSKPNALLAVALITPRNDYAKDWANSYNAVIPG
                     VVQTRINQGFNVILVDLYKNFPSNGLGSDNLHPNDTGYKFLGQTWYNAIKGYLK"
     gene            5312..6139
                     /locus_tag="PKZ52_05275"
     CDS             5312..6139
                     /locus_tag="PKZ52_05275"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNG59217.1"
                     /translation="MTDVDFTKTQQQDHSPAPATAHPRQRRVRAIFISDVHLGTKDSK
                     AALLNEFLKHYRSENLYLVGDIIDGWKMRAGVYWHRDFNKVIRRLLKLAKRGVSIKYI
                     TGNHDEFLRKHANHRFDNIHLINRCEHIDARGKCFLVLHGDQFENVTHAHRWLHYIGD
                     YGYDFLMWTNRWFNHWRAKYGFGYWSFAGFLKQHIQTAQTYILNYEDAVARGASHKGF
                     QGVICGHIHQAAHKNLQGITYLNTGDWVESCTAVVEELDGSWRLVYWLQERQAFINP"
     gene            complement(6445..8049)
                     /locus_tag="PKZ52_05280"
     CDS             complement(6445..8049)
                     /locus_tag="PKZ52_05280"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNG59218.1"
                     /translation="MMQYKKNILMVAAENDALPNAKVGGIGDVVRDIPIALAREECQV
                     QVVLPDYGHFSKMPSTEKVAAFAVNFAGKQEEVHLFKVKGKQLLKTYLDEVGPAITYW
                     ALGHSYFSPCGDGAVYCNDGSERPFATDASKYAFFCAAVAQVCVQGGFGALDVIHLHD
                     WHAALILVLREFDPAYASLKAIPTVYTIHNLSLQGVRPFAGDGSSLETWYPHLNYERS
                     RICDPRATHCINPMRAGITLADKVHTVSPSYAQEIQRPSFTARGVYGGEGLEQDLILA
                     NAQGKLVGILNGCEYPEGRKYKIENKNNLANIIIESLIGWVSKNPLVLSCHWVAQKRV
                     HLWLQNKERGFIVTSVGRITGQKVRLFKEIVDKSTGATLLDTILERLGTNGAFFLLGS
                     GTAEYEQFFLEVSARHPNFIYLQGYSDALSHALYNNGDLFVMPSSFEPCGISQMLAMR
                     AGQPCLVHAVGGLNDTVQDGITGFSFSGDDSVQQAQNCAKKFDEILTMHRDTPKTFQK
                     IATNAQAQRFSWEQVAKDYIEKLYAS"
     gene            complement(8427..8816)
                     /locus_tag="PKZ52_05285"
     CDS             complement(8427..8816)
                     /locus_tag="PKZ52_05285"
                     /inference="COORDINATES: protein motif:HMM:NF018574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HNG59219.1"
                     /translation="MSVEIPGKKIFLQHLETMFVNIESGPKWDMTQPMLWGYFFTHSE
                     PGRLEASIEKLKAYGLQLVAISLADKKTDDAPDVFRLHMAEVRAHSPQSLDERNNELY
                     LFAHSEGIDAYAGMDVKPVNKAVFEQA"
     gene            complement(8929..>9527)
                     /locus_tag="PKZ52_05290"
     CDS             complement(8929..>9527)
                     /locus_tag="PKZ52_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNG59220.1"
                     /translation="EKDATTEGSDHDFGKDIIPSIISKHDVQAFRFKEQDGERAYWRD
                     VGTLDAFWEANMELISPTPSLNLYDHHWPIWTYQTQLPPAKFVFDDDTRRGYAVDSMV
                     SGGCIISGGKVKRSLLFSDVHVHSYTSIEESVILPEVEVHRHVKIRRAIIDRACVIPE
                     GMEIGYNHDEDRARGFRVTKKGIVLVTPEMLGQRGGAR"
BASE COUNT         2159 a         2449 c         2611 g         2308 t
ORIGIN      
        1 cgccagtggg cacggtgagt ggggcgatgc ggttgtcaat agactcgccg cccgccaggg
       61 acgaattgcc gccgccaatg atcttggcgg aagtcaggca catgttgtcg ccggattggt
      121 gatcgcagcc agcgctgccc cagggttgat cgacgccgcg aaccaccagc aggcgcgatg
      181 cataatcggc gaacattcct accgtgcgct gcctttgctg gtcgtccgcc cgcagtgagt
      241 cgtaagtgag tggcccagtt tggctgggcc aaaacttctc tggctcgcct cggctcgaat
      301 ccgccatggc cacgccattg gcgccgacca cgaacagtgc aaatcggtct gtgaccgtgg
      361 ccgcttccgc atgcttagcg ctaaacgcat ccagtagcgg caggccgagg gtaattccgc
      421 ccgccccacg caggaaaata cggcgcgata gtgttgagtg gttattcttc atacttaact
      481 cctaaggttc gcgcgtcaga aaatattcac tttggaccag ggcgcggata acacccttgg
      541 ccgacaggtt ctgcgacttc gacttggctg ccagttgggt caaaagcgcg tcatcgccgt
      601 gcaccgcctg gcgtccgtag aggtaggaaa gccagtgctc ggcataacac tggtgggcca
      661 ggtcactctc tgctaactgc gcggttaatt cattggcgtt ggcaaactgt gcctcgaagc
      721 cattgttttg gtagacgtct gcagccttga ccggcaggcc gttgtcggtg gacctgaacc
      781 tgcccactgg gtcgtagttt tcgaacgcga agcccagcgg gttgatcacg gtggcatgac
      841 agcctgcgcc gcaggtgcct ggcccggtga ccgcgttgat gcggtcgcga ttggtctggc
      901 cgggtttaac ctcgggaagc ggtggaatgt tcgcagccgg cctacccacg tttacacaca
      961 gcacgtgttc gttaataaac acgccgcgat ggattggatc gggttggtac tcgccagcaa
     1021 acagggtgag aaagcccagc tgcatcagaa agcctgcgcg cccttgcgct gacaggtcca
     1081 ccttttggaa ctcgctgcca aagcctgctg gcaagccgta gaagggggct aagttcgcgt
     1141 ccacataggc gttgggcgag gtaagaattg ccctgacgcc caggttttca tcgaagatag
     1201 attggaaaaa taactccgcc gactttttca tcgaagtgac tgtcccggcc ccaaattgtg
     1261 ggtagttggc ggggttgcgc tgaataatgc tgtagcggga gagtgcgtag gtctgagtgt
     1321 gcaagtgcag ggcggtggct tgcgcgcggg gcgtcttgag caaccgctcg gcgtgatcgg
     1381 caactgcagt tgtggccttg ccaggatcga gtgcgcccga agcggcagcg tcgagcagaa
     1441 gcttatcggg gccgctgccg gtaatggcca gcgcgagttt tgcagccacc tcgtagccgg
     1501 ttaggggaat gcggccattg ctggtcactt gccccagctc cgtccggtac agaaagtagg
     1561 gggactggag gactgcttca actaccagcc gaactccgcc agcgaacgca cccagttctg
     1621 gaaacagctg tgggcctttt tggaacagtg cgtagtagcg gtcggactca gccgtggtca
     1681 gcgggcgacg atgggctcgc agccctaggt cgttgataaa cgcccgcgcg cgggcggtgg
     1741 cttccgttgg cgcaccctgg ggcacaagcc ggttgatcgc cgtggtgctt gccgccacct
     1801 gggcggctag tgcttctgcg gcattgcggt agtccccgcg caaagtgctc gatacacgca
     1861 gttgcgtagc attggtgtcg tagccgacga aggcatccgg ggaaaagctc tccacctgct
     1921 cattgggcgg cgcgtctaaa tataggaggt cttggacggc gtttttccat tgaaaatgcg
     1981 tcagcctggc aatgcgcggg acgatgagtt ttggctctgt aaacgtcgcc agtatgtaag
     2041 ccgccacgtc ggtggcacag gagcccttac acaatgcgcc gcgcagcggc atcgaagttt
     2101 cgattttaga aacgagttct ttataggatg tgcatgcttc acacgagggc agcgtcagcg
     2161 cagggtaagc gccggagccc tcaccactag cgccatggca ggtttcacac tgggtggtgt
     2221 ataggccttt accgatagaa actgaacctg ggttttcccc atcggcaact ttttcctcac
     2281 gaaaaacctc gattgccgca agcgttccgt tatcgaccgt tgcggaaacc acaatggata
     2341 aattgccgtc gctcacctga acaattggcg attcaacatc gtgggccacg tcgtggccaa
     2401 acgaggcgaa caagtcgata ttgctgacaa cttgttgccc ttcaaccgac acactaaatt
     2461 ttcgcgcggc aatggccgtt tgataaattt ccgccatttt taacgtcacg cgatagcggc
     2521 cagccgccac cggtaactga tacgaataat taccgtagcg ctcggtttga tagagcgcgt
     2581 catcgctggt attagcaatt gcatcgacgg tactcgtaac ggtgccgccg acaaagtctc
     2641 tatctttttc gtaccggtaa cccgatgccg ttacaacctc gtcacccccc acattaattc
     2701 caaaagcccg ttcccaacga aaactaggag ccgctccacc tgaagacgat gaggacgatg
     2761 aagaagacga actagaggaa gaggagccgc tggacgaact actgctggag ctggaggaag
     2821 aactcgttgc ggccggttgg gcaccgcagc cgctgagcag cagtgtgcag accccgctca
     2881 agagtagctg ccttaaaggg ctggttaaat tcatagagga cgccttttta ttgttcgaat
     2941 ggggaatgca ggctccagat ggcggtgcgg cggaagctct gtttaattgt caaatgataa
     3001 gtctttatat caagggcaaa acagccgcca aatagtcaaa acgcctataa agcagcgttt
     3061 tttttgtgag gacatgggca tttttaacaa atgacatttt tatcggccgg tgaaaaacac
     3121 ccaatggcat aagtctgctc catgttatgc ctttggggtt tgacgctggg gtcgagagca
     3181 atcgctgctg gggcgaggga caaaagattt gcgcagcgtt gataggatta gtagctgcaa
     3241 cagtgaaaag gaaaacgcgg tgcgatcgcg aaaaaactct gcgccatcaa ggcgcgattt
     3301 tttaggttaa atggttcgaa ggcgcaggcg tttgcaataa cctgagctgc ctcctcccct
     3361 catctcttac cccatatcgc cctgatcttt tatcagattt ttgcacagcc tctcagactg
     3421 cccaggcaag ttcgaggatg ataatcgtat gaccataata tttttggctg gccgcatcgg
     3481 atgtgtttga ggagtagctg cgcggctcac aataacgatt aacaaagccc actactttgg
     3541 aggacttcac cttgaaaatc cggcaattaa ttcccttctc gctgtttacc ctgttgatcg
     3601 cctgcggtgg cgagccaggt actgggtcct cgtcctccag tagctcatcc agttcatcgt
     3661 ctagctcatc cagttcttcc aattcaactt ccagctcgtc aagttcgtct tcgagctctt
     3721 caagttctag ctccagctca tccagctcca gcagttcttc cagcagtggt gtggcaggca
     3781 gtttggtgta cgccatagat gtgggcggct ccagcccgat gaccctggat ggggtgcgct
     3841 acgaggcgga tcgtttctca tccggcggca cggttaactc cactacagat cctatttccg
     3901 gcaccactaa ttccgcgctg taccaaacgg agcgttacgg cacctttagc tactcgattc
     3961 ccgtcagcaa agccacctac agtgtggtgc tcaacctggt ggagctatac cacaccagcg
     4021 cgggcagccg cagcttcagt gtggtggtgg aagggcagac ggtggcaacg ggtttggata
     4081 ttttcagcaa agtgggccac gacagcgcct atgttctgcg ggccaataat atcccggtaa
     4141 ccgacggcgc cttaaccatt cagctcacct cccaggtgga taacgccacc ctggccggtt
     4201 tagctattta ttctgccgac ggtggccgtt tggaagagcc accggcaccc accatctgcc
     4261 ccgccagcgg cccgtgcaaa attttgcccc tgggggattc cattaccgat ggtgtgggcg
     4321 gcagtggcgg cgcctaccgg gtggaattat tccgccaggc ggtgctgaat aataaaaaca
     4381 tcacctttgt gggctctaag tccaatggcc ccaccactgt tgagggcaag agcttcccgc
     4441 gcagccatga aggtaatagt ggatattcaa ttaaccaaat cgccaatcta gtacccagcc
     4501 cggcccttaa cggcgatccg aatattattc tgctgatgat tggcaccaac gactcgtggg
     4561 tggaacctaa taacaatggg gcacaagcta tggcgcagag attgggcgcc ttgatggaca
     4621 aaatcatcaa cagcaaaccc aacgcgctgc tcgcggttgc cctaatcaca ccgcgcaacg
     4681 actacgccaa agactgggcc aattcttata acgcggtcat tccaggggtt gtgcaaacgc
     4741 gtatcaacca aggctttaat gtcattctgg tggaccttta caaaaatttc cctagtaatg
     4801 gcctgggatc ggacaacctg caccccaacg atacgggcta caaattttta gggcagactt
     4861 ggtataacgc tattaagggt tatttaaagt aatttaaggg caaatttttt agctggcact
     4921 caatttaaat tctgtggtta aacctaaaaa tcgccttagg gtccaaccgg ggcttttaga
     4981 ttaaagacga gtggccaggt aagtatcttt tccttaacta ttcacccagc ggctttttat
     5041 gccgggttag ctttttatat ccggtgagta cggcggcaag gaagccgcac caaaggcgca
     5101 agccgccgcg agttgcgtta aaaacacgaa aacccatgca tattcgcgcg catcagtgcc
     5161 gcgaaagcaa aatataccag gacgatctat tctgccgccg ggttactacc taggcaccag
     5221 caaagaatcc acctagtgtt gattgcttgc cctaaacgct ttgcacaccc aatttcatca
     5281 agctgtcatt tcgccaccgc acactcacgt gatgacagat gttgatttca ccaagaccca
     5341 gcaacaggat cactcgcctg cacctgccac ggcccatccc aggcagcgcc gtgtgcgggc
     5401 tatttttatt tctgatgttc acctgggcac caaagacagc aaagccgccc tgctcaatga
     5461 atttttaaaa cactatcgca gcgaaaacct gtatttagtg ggcgatatta ttgacggctg
     5521 gaaaatgcgc gcgggggtgt attggcatcg agattttaat aaagtgattc gccgcttatt
     5581 gaaattagcc aagcgcgggg taagcattaa atatattacc ggcaaccacg atgaattttt
     5641 gcgtaaacac gccaatcatc gctttgacaa tatccattta atcaaccgct gcgaacatat
     5701 cgacgcacgg ggcaaatgtt ttttagtgct gcacggtgat caatttgaaa acgtgaccca
     5761 tgctcaccgc tggctgcatt atatcggcga ttatggctac gattttttaa tgtggactaa
     5821 ccgttggttt aaccactggc gcgcaaaata tggctttggt tattggtctt ttgcaggctt
     5881 tttaaaacag catattcaaa ccgcacaaac ctatatcctc aattatgaag atgccgtggc
     5941 tcgcggggca agccacaagg ggtttcaagg agttatctgc ggccacatcc atcaagccgc
     6001 acacaaaaac ctgcagggga ttacctactt aaacactggc gactgggtgg aatcgtgcac
     6061 agctgtagtt gaagaattgg atggcagttg gcgtttagtg tattggctgc aggagcgcca
     6121 ggcttttatt aatccgtaag ctgggataac aaagcacccc accattgctc ttgcggcggt
     6181 gaattattat tttgagcgcc gcaattgagg ccgagaaatt aaatgggggg cgcttaattg
     6241 cggaggaagg tgtagggtgg attcggagcg aagcgacaac ccaccactgc cccgcgactc
     6301 tcactcaaaa tgataattct tagtggggat cacgatggtg ggttgcttcg cgaacccacc
     6361 ttacgcgttg ccactattga atggcaaacc cagatttcgc ttgcgccaca tccaggccac
     6421 ccagtgtgtt tatttttagg cgcctcagct cgcgtataat ttttcgatgt aatccttcgc
     6481 cacctgctcc caagagaagc gctgcgcttg ggcgttggtg gcgatttttt ggaatgtttt
     6541 gggggtgtcg cggtgcattg ttaaaatttc atcgaatttt tttgcgcagt tttgcgcttg
     6601 ttgcacgctg tcgtcgccgc taaaactaaa gccggtgata ccatcctgca ctgtgtcatt
     6661 taagccgccc acggcgtgca ctaaacaggg ttggcctgcg cgcatggcca gcatttggct
     6721 gatgccgcag ggctcgaagg agctgggcat aacaaataaa tcgccgttgt tatacagggc
     6781 gtgagagagg gcgtcggagt agccttgtaa ataaataaaa ttcgggtggc gggcgctcac
     6841 ctctaggaaa aattgttcgt attccgccgt gccactacct aaaagaaaaa aagcgccgtt
     6901 ggtgcctagg cgctccagta tagtatccag caatgtcgcg ccggtggatt tatccacgat
     6961 ctctttaaat aaacgcactt tttgcccagt aatacgcccc acagaagtga cgataaaacc
     7021 gcgctcttta ttttgcagcc acaggtgtac ccgtttttgc gccacccagt ggcagctgag
     7081 cactaagggg tttttgctca cccagccgat caggctttca ataataatat tggcgagatt
     7141 atttttattt tcgattttgt atttgcgccc ttccgggtat tcgcagccat ttaatatgcc
     7201 cactaatttg ccctgggcgt tggcgagaat taaatcctgc tctagccctt cgccgccgta
     7261 tacgccgcgg gcggtaaaac tcggccgctg tatttcttgg gcgtagctgg gggagacggt
     7321 gtgtaccttg tcggcgaggg taatgcccgc ccgcatgggg ttgatgcagt gggtggcgcg
     7381 ggggtcgcag atgcggctgc gctcgtaatt taaatggggg taccaggttt ccaacgagga
     7441 gccgtcgcca gcaaaggggc gcacgccctg cagggataaa ttgtgaatgg tgtaaacagt
     7501 gggaatggct tttaaactgg cgtaggccgg gtcaaattcc cgcagtacta aaattaatgc
     7561 ggcgtgccag tcgtgcaggt gaatcacatc taaagcgcca aagccgcctt gcacacacac
     7621 ctgggcaacg gcggcgcaaa aaaaggcgta tttgctggcg tcggtggcaa aaggccgctc
     7681 ggagccgtcg ttgcaataca cagcgccatc gccacagggg gaaaaataac tgtggcccaa
     7741 ggcccaatag gtaatggctg ggcctacttc atctaaatag gtttttaata attgtttgcc
     7801 cttaactttg aataaatgca cttcctcttg tttgcccgca aaattcaccg caaaggcggc
     7861 gactttttcc gtgctgggca ttttagagaa atggccgtag tcgggtaata ccacttggac
     7921 ttggcattct tcacgggcca gggctatggg aatatcgcgc actacatcgc ctatgccgcc
     7981 gactttggcg ttgggcagag cgtcgttttc agcggcgacc atgaggatat tttttttata
     8041 ctgcatcatt ttatttcgag tctaattagt aggctttgct tgctcgcaac cggttcgatg
     8101 attgagtgcg taccggtttt tacggcgggt tgtttttacc taacaaccac agttgaactc
     8161 gtagcgaggg cgactaaggc gctgaatatg cgcgcttttg ggtaaaaatt gggtgaaagc
     8221 agtagtcgct gctttggcga gtccaatttt taccaaaaaa cgtgcagatt caagcattag
     8281 gcagccgcag tagagttcaa ttgtggtttt taagtttaag taaagcgtgt atttgggttg
     8341 gtttaaatgg cgtgttgcac aattgagact taggaccggt ttggttgtta ttaaacgccg
     8401 ctaaacgcta tttttagtgg cgctatttag gcctgttcaa agacagcttt attcaccggc
     8461 tttacatcca taccggcata ggcatcaatg ccttcgctgt gggcaaatag gtacagctca
     8521 ttattgcgct catctaagga ttgcgggcta tgtgctctaa cctccgccat atgcagccgg
     8581 aacacatcgg gcgcgtcatc cgttttttta tcggcaagcg aaatagcaac caattgcagg
     8641 ccgtaggctt tgagtttttc tatcgatgcc tcaaggcggc cgggttccga gtgggtaaaa
     8701 aaataccccc acagcatggg ctgtgtcata tcccatttgg gcccgctttc gatattcaca
     8761 aacattgttt ctaaatgctg gaggaatatt tttttgccag gaatttcaac cgacattatt
     8821 ttctcttcct tatctcataa gtattgagct taggccttat tgaccctgcc gggattaacc
     8881 cggatttcgc ttgcgcttca tccgggctgc cgtgtaagtg cagggaattt aacgcgcccc
     8941 gccccgttgg ccgagcattt caggggtgac taacactatg ccttttttgg tgacgcgaaa
     9001 gccgcgggcg cggtcttcgt cgtggttgta gccaatttcc atgccctcag ggatcacaca
     9061 ggcgcggtcg atgatggcgc ggcggatttt gacgtggcgg tgcacttcta cctccggcag
     9121 gattaccgat tcctcaatgc tggtgtagga gtgcacatgg acatccgaga agagcaggga
     9181 gcgtttcacc ttgccgccgg aaataataca gccgccggac accatggaat ccaccgcgta
     9241 gccgcgtcgg gtgtcgtcgt cgaagacgaa tttggcgggt ggtaattgag tttggtaggt
     9301 ccagatgggc cagtggtggt cgtagaggtt taacgagggc gtgggggaga taagctccat
     9361 gttggcttcc cagaaggcat ccagcgtacc cacatcgcgc cagtaggcgc gctcgccgtc
     9421 ctgctcttta aagcggaagg cctgtacatc gtgtttgctg atgatagacg ggatgatgtc
     9481 tttgccgaag tcgtggtcgg agccttcggt ggtggcgtct ttttcca
//
LOCUS       DAOQGS010000096         9518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3374_length_9518_cov_4.782838, whole genome shotgun sequence.
ACCESSION   DAOQGS010000096 DAOQGS010000000
VERSION     DAOQGS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_9518_cov_4.782838"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PKZ52_05295"
     CDS             <1..1336
                     /locus_tag="PKZ52_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HNG59221.1"
                     /translation="SPSIYPTGVTRYNPSKAYNSYVLFSGQDRKTHLIDLNGNEVRQW
                     NYYGFPPVFIDPKLVGGKKGHVLLQLTGETGGRANAGDPQAIGEVDWDGKIIWQWSGV
                     KSQDAYANGNISTDASGIGNNIRHHHDWRKLPNGNTLILVNYQRPVTGFKAESLLEDG
                     IYELTPKGEIVWQWAAFDHFNELGFSEASQKLIRESLSRNDKKVPFDYLHFNNMSVVG
                     PNKWFDGGDTRFHPDNIVVCAREANFTAIIDKKTGKIVWRIGPDYPPITTPNKLPRPV
                     DQITGQHDAHIIPKGLPGEGNLLVFDNQGEAGYPPVKLNTQPGSRVLEIDPIKNEIVW
                     QYTGVDSGAPNYTFYSSYISSARRLPNGNTLIDEGMNGRFIQVTPQGEIVWEYISPYF
                     SQANPKDEKPTINNSVYRAQPVPYDWAPAGTPHSEKPLKALDVSQYRLPVEK"
     gene            1339..2769
                     /locus_tag="PKZ52_05300"
                     /pseudo
     CDS             1339..2769
                     /locus_tag="PKZ52_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(3022..3765)
                     /locus_tag="PKZ52_05305"
     CDS             complement(3022..3765)
                     /locus_tag="PKZ52_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59222.1"
                     /translation="MKISKILIVVSSVLLLNSNVFGAEPAKEVNKPPAPAGNPGAAPI
                     SPEEAKKRFSFFVTSAGLGKGADLGGLAGADAHCQKLASSVGAGDKTWHAYLSTQAEG
                     NKPAVNARDRIGKGPWYNVKGALIANDLAHLHGDTLDLARLGSNLSRNQVFTEKGDKI
                     PGAGDTPNQHDILTGSKTDGTAFTDTADHTCKNYTSSAVDGSAQVGHFDRTGGGNTSW
                     NSAHGSRGCGQENLVATGGAGLFYCFAVN"
     gene            complement(3993..4445)
                     /locus_tag="PKZ52_05310"
     CDS             complement(3993..4445)
                     /locus_tag="PKZ52_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59223.1"
                     /translation="MNTSLKIFTLLCALSASAVSFAEDANQKPKVKYKTPELTVAQVD
                     EWLGKPEQVLLVDIRRPDELISFGSFPVFLSVQFKDLEKLIPYIPKERSIITVSNHAN
                     RAFAAGDLLSSKGYKVIGAAGSENYEKSGGKQVTKIEKPAPKPATEQK"
     gene            complement(5337..6104)
                     /locus_tag="PKZ52_05315"
     CDS             complement(5337..6104)
                     /locus_tag="PKZ52_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59224.1"
                     /translation="MFSFFTPKPIVSPASAAWIEACFAWANEHFDGHKFLHNTRLIQP
                     TNQDFPGRVDSPTAMAATVLNHVMNYSGVNQWPLRLAHLTAEAPSLPPLLPLNPNQRS
                     GDLAAQQWAPGEFLPVYFHPEQCKQPGDLAATFATVLAQHMIIQARQTPPGGLEFFSN
                     AAEVLAVFLGFGVLLANSAYNFRGSCAKCYNPMANRQAELTEAEVVYALALFINSKNI
                     PPKEATRHLKPHLKSLLKKADRYIAWQRAENKANLIS"
     gene            complement(6267..7844)
                     /gene="guaA"
                     /locus_tag="PKZ52_05320"
     CDS             complement(6267..7844)
                     /gene="guaA"
                     /locus_tag="PKZ52_05320"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNG59225.1"
                     /translation="MSTNIHAQRILILDFGSQYTQLIARRIREIGVFSEIRAFDMSED
                     EITAYQPAGIILAGGPESVTEGASPRAPACVFTLGVPVLGICYGMQTMAEQLGGKVES
                     SSVREFGYAQVQVHGQAQILADIKDHLGAQGESLLDVWMSHGDKVVAMPEGFALMAST
                     PSCAIAGMYHPQKHLYGVQFHPEVTHTLQGKRLFEHFVLQICRCPAVWTPANIVEDAI
                     AKVRAQVGSDQVLLGLSGGVDSSVVAALLHKAIGPQLTCVFVDNGLLRKNEGDQVMDM
                     FAKNMGVRVIRADAEDLFLSKLQGENDPEKKRKIIGAAFIDVFDQEAKKLNGINWLAQ
                     GTIYPDVIESAAAKTGKAHVIKSHHNVGGLPDTMKMQLVEPLRELFKDEVRKIGLELG
                     LPYDMVYRHPFPGPGLGVRILGEVKKEYADILREADAIFLEELHQAGWYHKTSQAFAV
                     FLPVKSVGVVGDGRRYEWVIALRAVETIDFMTARWAHLPYELLEKVSNRIINEISGVS
                     RVTYDVSSKPPATIEWE"
     gene            complement(7917..9392)
                     /gene="guaB"
                     /locus_tag="PKZ52_05325"
     CDS             complement(7917..9392)
                     /gene="guaB"
                     /locus_tag="PKZ52_05325"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNG59226.1"
                     /translation="MLRIAQEALTFDDVLLIPGYSEVTAKDVSLKTSLTRKISLNIPL
                     VSAAMDTVTESRLAIALAQEGGIGIIHKSMSIEQQAAEVHAVKKFEAGVVRAPITIES
                     SASINDLIKLTRQHRISGVPVMEKGNLVGIVTGRDVRFESNLNATVASIMTPKEKLVT
                     VKEGASADEVRGLLHRHRIEKVLVVDDAFNLTGLITVKDINKAETYPNACKDDLGSLR
                     VGASVGTSPDTDDRVAALVAAGVDVLVVDTAHGHSKNVLDRVRKIKREYPHIQVIGGN
                     IASADAALALVEAGADAVKVGIGPGSICTTRIVTGVGVPQISAIANVAEALRGTDVPV
                     IADGGIRFSGDIAKAIVAGAHVVMMGSMFAGTEEAPGEVELYQGRTYKSYRGMGSLGA
                     MAKTQGSSDRYFQDSSQGMEKLVPEGIEGRVPYKGPLQAIVHQLMGGVRAAMGYTGSQ
                     DLPTMRTKPQFVRVTTAGVGESHVHDVSITKEAPNYPVSGR"
BASE COUNT         2459 a         2398 c         2272 g         2389 t
ORIGIN      
        1 aagccccagc atttacccca cgggggtcac ccgctacaat cccagcaaag cctataattc
       61 ttacgtattg ttctcaggcc aggatcgaaa aacccatctt atcgatttaa atggcaacga
      121 agtgcgccag tggaattatt atggctttcc accggtattt atcgatccta aattggtagg
      181 cggtaaaaag gggcatgtgt tattacaatt gacgggtgaa actggtgggc gcgccaatgc
      241 cggtgaccct caagctattg gcgaagttga ttgggacggc aaaattattt ggcagtggag
      301 cggtgttaaa agccaggatg cctatgccaa cggtaatatc agcaccgacg ccagcggtat
      361 aggtaataac attcgccacc atcacgactg gcgcaaattg cccaatggca acactttaat
      421 tttggtgaat taccaaagac ctgtgaccgg ttttaaagcg gaaagcctat tggaggacgg
      481 catttatgaa ctgaccccga aaggagaaat tgtttggcag tgggcggcgt ttgatcactt
      541 taacgaattg ggctttagcg aggcgtcgca gaaactaatt cgcgagtcac tctcgcgcaa
      601 cgataaaaag gtaccttttg attatttaca ctttaataat atgtcggtgg tggggcccaa
      661 caaatggttc gatggcgggg atacgcgctt tcatccggat aatattgtgg tctgtgctcg
      721 cgaagcaaac ttcaccgcca taatagacaa aaaaactggc aaaattgtct ggcgcatcgg
      781 cccagattac ccccctatta ctacacccaa taaattaccg cgcccagttg atcagataac
      841 cggccaacac gacgcccaca ttatcccaaa aggcttgccc ggcgaaggca atttattggt
      901 ttttgataac cagggtgagg ctgggtatcc gccggtaaaa ttaaatactc aacccggttc
      961 acgggtatta gaaatcgacc cgattaaaaa tgaaattgtt tggcaatata cgggggttga
     1021 ttccggggcg cctaattata cgttttacag ctcatatatc agcagtgctc gccgcttacc
     1081 caatggcaat actctaattg atgaaggcat gaatggccgc tttatccagg taacaccgca
     1141 aggggaaatc gtctgggaat atattagccc ctactttagc caggccaacc cgaaggatga
     1201 aaaacccacc atcaacaatt cagtctatcg cgcccaacct gttccctacg actgggcacc
     1261 cgctggcact ccgcattcgg aaaaaccgct aaaagcgctt gatgtcagcc aatatcgcct
     1321 acccgtggaa aaataaccat gagctctcgc cgtaccttgc gccgacgttt attgcgcaat
     1381 ttatcttttt tttccttggc ggcagttagt ggtggtgtgc agtctgcact agctgccacc
     1441 aagaaaaacg ccgccagcaa accaaatatt atttttattc tcgccgatga tttgggtttt
     1501 gctgatttaa gcatttatgg acaaactgac ttcgccactc ccaatttaga tcggttggcc
     1561 caagagggcg tgcgttttaa ccaggcctat gcaaattctg ctgtgtgctc ggcgacccgc
     1621 tttggcctta tcaccgggcg ctatcaatag cgtttgcgca gcggccttga agagcccctg
     1681 cgcggcgata cccaggccct cggcctgccg ccatcccatc ctacactgcc ctcgttattg
     1741 aaaaaagccg gttataaaac ggcgctgttt ggcaaatggc atttgggtgc actgcccaag
     1801 tttagtccgc taaaaagtgg ttacgataaa tttttcggca acttcggtgg tgccattgat
     1861 tatttcactc ataaaaatgg caccgatgca aaagccaaat tcgatctata cgaaaatgaa
     1921 actccagtgc agcaagtggg ttattacacc gatttaattg ctgaacatgc ccaagcttat
     1981 attgctgaac aaaacggcaa aaaacccttt ttcctctcct tacattttac cgccccccac
     2041 tggccctggg aggggccgga tgatcaggcg gtagccaact cactttccaa ccttggccat
     2101 tatgatggcg gcagcctggc cacctacgca aaaatggttg gctccctaga cgatgctgtc
     2161 ggcaaggtat taaccgcatt aaaagaaaaa aacatcgctg acaatacgat tgtgattttc
     2221 accagtgata acggcggcga acggttttcc cgcacctggc ccctaagcgg gcaaaaatcg
     2281 gaactgttgg aaggcggtat tcgtgtaccc gccattgtgc gctggcccgc ccaagtaaaa
     2341 tctggccgcg tgagcgagca ggtaattatc agtatggatt ggctgccgac actgctagca
     2401 gctgcaggcg gtgcgcccga tacaaagttt aaacccgatg gcgagaatat tttaccggcg
     2461 attttagata cagcaccgcc ctacccgcgc actttattct ggcgctacaa agccaacgag
     2521 caagcagcca tccgcttggg tgactggaaa taccttaaaa ttgctggcaa tgaattctta
     2581 tttaatttgg cccaggatgt acgcgagcgg gcaaatcttg cacaaaaaca gccggaaata
     2641 tttaaccaac tgaaacagcg ctggcaggat tggaataata ccatgctgcc tattacagca
     2701 gatgtgcgct cctacaagat cgatagcaag tcgcaagcgg atcactatcg cggacaagac
     2761 ggtctttgag cagatattta ttagcaccga gttttgcctg tttagcattg cagctgcttt
     2821 actacagaac tcggttttaa aagcattcaa acatttaact gtcgtttgat tgccaggcaa
     2881 tttttctcaa actggagata tttatctata atatgattgt ggacaactag gttgctacga
     2941 cagcggaaat taaattttcg cttgcggatt cagaggcccc taggatttaa actgtagaat
     3001 tcatccttga attcactgtg atcaattcac cgcaaaacaa taaaatagtc cggcgccacc
     3061 tgttgcaacg aggttctctt ggccgcagcc tcggctgccg tgcgcggaat tccaagaggt
     3121 attgccacca cctgtgcggt caaaatggcc tacctgtgca gatccatcaa ctgccgaact
     3181 cgtgtagttc ttgcaggtgt gatcggcggt atcggtaaag gccgttccat ccgtctttga
     3241 gcccgtcaaa atatcgtgct ggtttggtgt atcgccagcg ccgggtattt tatcgccttt
     3301 ttcagtgaac acttggttgc ggctaagatt acttcccagg cgcgcaaggt cgagtgtatc
     3361 gccatgcaga tgcgcaagat cattggcaat taaagccccc tttacgttat accaaggccc
     3421 tttgcctatt ctgtctcttg catttaccgc gggtttgtta ccttccgcct gagtgctcaa
     3481 ataggcgtgc caggttttgt caccagcgcc cacgctggat gccaattttt gacaatgggc
     3541 atcagcgccc gcaaggccac ccaaatcagc gccttttccc aatcctgcac tggtaacaaa
     3601 aaagctaaag cgttttttcg cttcttctgg gctaatgggt gcagcacctg ggttgcccgc
     3661 aggcgcaggt ggtttattta cctctttggc tggctccgcc ccaaacacat tagaattgag
     3721 caacaacaca ctactgacaa caattaatat tttagatatt ttcatgcaac tccaccgatg
     3781 gatagattga aacaaatcaa caataacttt ttatttcaaa ataataaaaa attcatttga
     3841 tttttagttt acacaagata aaaccgatcg ccttaaaggc tgaatgcctg caacaagcct
     3901 aacctcaaca cagcgcactg ctcagcagag caacccagca acggctaaac cattgcgggc
     3961 catagcattt atcagcccga gggatctatt tattattttt gttcagtggc aggtttggga
     4021 gctggttttt ctatttttgt cacctgctta ccgccggatt tttcatagtt ctcggagcct
     4081 gcagcgccaa tgactttata gcctttgctg gagagcagat cgcccgcagc aaaggcgcga
     4141 ttagcatggt tcgatacggt gataatactg cgctctttgg gtatgtaggg aataagtttt
     4201 tccaagtctt taaattgaac gctcaaaaat accgggaaac tgccaaaact aattaattca
     4261 tccgggcggc gtatatctac taaaagcact tgctcaggct tgcctaacca ctcgtccacc
     4321 tgtgcgacgg tgagctccgg agtcttatat tttacctttg gcttttgatt cgcgtcctcg
     4381 gcgaatgaga cggctgatgc agagagcgca cacaataggg tgaatatttt taaagaagtg
     4441 ttcatgactg taattccaga ttaaagttaa taactgaccc atttagaatg gtaaatgcaa
     4501 atacaatttt gattgtgaat tttttgcggg gtcagcaaag caattagtat tccaaatcag
     4561 cccatgcctt tagctgagca aaaatagatc attttggcct tttatctgat tcacatcagc
     4621 tgataattaa gcagcctagc ccttcggatg attatcctgt ttttatacaa acagttggca
     4681 gctgaaattt caacaggact cttattgaga cttacccatg gttatggcca ctctaattca
     4741 gcaggaatta ttgaacatag tacaatcaca caattacttg cttgcaattt attcgcttct
     4801 agatttaatc acggcgctct aaaatcatgg agccgccaag catagttata tattttctgc
     4861 taggagccat cttattaacc cggcactaaa cgccgccggg ttaatattta gcattaacgg
     4921 ttacacgcgc agtccaagac ggctcaggcg cgaatagaac aaacacccgg aatgacattt
     4981 tttaacagcg cgattaagat gccacacaga taacgcgcag caacttatgc attgcagtaa
     5041 aagagcattt attgttatta ggccgggctt gcgttcgggt aaaaatgctt tcagataatt
     5101 gatatgcggc aaaagtatct agccatagga gagatacggg atatttatac aaggcaggcc
     5161 acagtttatt tttggccgtc agacatgcat cttaaggtta ctttttataa gcaacctccg
     5221 attaattgat cttttcggaa cccttgtgtg cagaaagccc tgcggcgcgg ctacgagaat
     5281 gccgcccaaa tcagagcatc ccgttgatac ctaagcagtg ggaaaacgat attccctcag
     5341 ctgataaggt ttgccttatt ttcagctcgc tgccaagcaa tatagcggtc ggcctttttc
     5401 agcagagatt ttaagtgggg ttttaaatgg cgtgtggctt cttttggcgg gatattttta
     5461 ctattgataa acagtgccaa ggcgtacacc acttctgctt ccgttaattc cgcttggcga
     5521 ttggccatgg ggttataaca cttagcgcag ctaccgcgaa agttataggc ggaattggcc
     5581 agcagcacac caaaacctaa aaatactgct aacacctcag ccgcattact aaaaaactcc
     5641 aacccacccg gcggggtttg gcgggcttgt attatcatat gttgggctaa tacggtggca
     5701 aaggtggcag ccaagtcgcc gggttgtttg cactgctcag ggtgaaaata tacgggcaag
     5761 aattcaccgg gcgcccattg ctgggcggcc aagtccccac tacgctgatt ggggttgagg
     5821 ggcaacaatg gtggcaggct gggtgcctcg gcggttaaat gagccaagcg caatggccat
     5881 tggtttacac cgctgtaatt catgacatga tttaacacag ttgcagccat ggccgtgggg
     5941 gaatccactc ggcctgggaa atcttgatta gtcggctgaa ttaaacgcgt attgtgtaaa
     6001 aacttgtggc cgtcaaaatg ttcattggcc caagcaaaac aggcttcaat ccaagcggcg
     6061 ctggctgggg atactatggg cttgggtgtg aaaaagctaa acatggcacg gcctaaaaca
     6121 attccaaata aatctgccct tctgcagatt tattgaaggg ttgaaagcgg gttgacccgc
     6181 cgcccaaaac actgataata aaacccgacc ggctaacgag taggtctgct gagcacccga
     6241 caaaaaacgc gcccaacaaa ttaaaattat tcccactcga tagtggcagg cggtttactg
     6301 ctcacatcat aggtaacgcg ggatacgccg gaaatttcat taataatccg gttagagacc
     6361 ttttccaata attcataggg caaatgcgcc cagcgggccg tcataaaatc aatggtttcc
     6421 accgcacgca acgcaataac ccattcatag cggcggccat cgcccactac acctacagat
     6481 ttaaccggta aaaacaccgc aaaggcttgg ctggttttgt gataccaacc ggcttggtgt
     6541 aattcttcta aaaagattgc atcggcttcg cgcagaatat cggcgtattc ttttttcacc
     6601 tcacctaaaa tacgcacacc caggcccggg cctgggaagg gatggcggta caccatgtcg
     6661 tagggcaggc ccaactccaa gcctattttg cgcacttcgt ctttaaataa ttcccgcagc
     6721 ggttcaacta attgcatttt catagtgtcc ggcaagccgc ccacattgtg atgggattta
     6781 atcacatggg ctttaccggt tttggcggca gccgactcaa tcacatcagg gtaaatggtg
     6841 ccctgggcca gccagttaat gccgttgagt tttttagctt cttgatcgaa tacatcgata
     6901 aacgccgcgc caatgatttt gcgttttttc tccgggtcgt tttcgccttg taatttactt
     6961 aaaaataaat cctcggcatc ggcgcgaatt acccgcacac ccatattttt agcgaacata
     7021 tccatcactt gatcgccttc gtttttgcgc aacaaaccgt tatccacaaa tacacaggtt
     7081 aattgcgggc cgatggcttt gtgtaacagc gctgccacca cggaggaatc cacaccgccg
     7141 gataaaccca ataacacctg atcactccca acttgggcgc gcactttagc gatggcatct
     7201 tccactatat tggcaggcgt ccacacggcg gggcagcggc aaatctgcag aacaaaatgt
     7261 tcaaataaac gcttgccctg cagggtgtgg gtcacttcgg ggtgaaattg cacgccgtat
     7321 aaatgttttt gcgggtggta catgcctgca atggcgcagc tgggagtaga cgccatcaga
     7381 gcaaaacctt ccggcatggc aaccacctta tcgccatggc tcatccaaac atcaagcagg
     7441 ctttcaccct gagcgcctaa atgatctttg atatccgcca gtatctgagc ctggccgtgc
     7501 acctgcacct gggcgtaacc aaactcacgc acgctggagg attccacctt gccgcccagt
     7561 tgctccgcca tggtttgcat gccgtagcaa atgcccaata caggcacgcc tagggtaaat
     7621 acacaggcag gtgcgcgggg cgatgcgcct tcggtaactg attccggccc gcccgctaaa
     7681 ataatacccg ccggttgata agcggtaatt tcatcttcgc tcatatcaaa ggcgcgaatt
     7741 tcagaaaaaa cgccaatttc acgaatgcgg cgggcaatta attgagtgta ctgggagccg
     7801 aaatctaaaa ttaaaatgcg ctgggcgtga atattggtgc tcataaattc tcttcgtggt
     7861 gataaaaaga aaacccgcca aagcgggttt tagtcaaaac aaaaggggcc aattatttaa
     7921 cggccactga ccgggtaatt gggcgcttct ttagtgatgc ttacatcgtg cacgtggctt
     7981 tcgcccacgc ctgcagtggt cacgcgaaca aactgcggtt tggtgcgcat ggtgggcaaa
     8041 tcctgggagc cggtataacc catagcagcc cgcacgccgc ccattaattg atgcactatg
     8101 gcttgcagag gccctttgta gggaacgcgg ccttcaatac cctcgggcac cagtttttcc
     8161 ataccctggc tggaatcttg gaagtaacgg tcggatgaac cctgggtttt cgccatggcg
     8221 ccgagggagc ccatgccacg gtaggattta taggtgcggc cttgatacaa ctcgacctcg
     8281 ccaggagctt cttctgtgcc tgcgaacata gagcccatca tcaccacatg ggcaccggcc
     8341 actatggcct tggcgatatc gccggaaaag cgaataccgc cgtcggcaat taccggtaca
     8401 tcagtgcctc gcagagcttc tgccacattg gcaatggcgc tgatttgcgg tacgcctacg
     8461 ccggtcacaa tgcgggtggt acaaatggag ccagggccta tgcccacttt tactgcgtca
     8521 gcgcccgctt ccactaaggc caaagcggca tcggcgctgg cgatattgcc gccaatcacc
     8581 tgaatatgcg gatattcacg cttaattttg cgcacgcgat cgagcacatt tttcgaatgg
     8641 ccgtgggcgg tatccaccac taacacatcc acaccggctg ccactaatgc ggctacacgg
     8701 tcgtctgtat cggggctggt gcccacactg gcacccaccc gcaaactgcc caggtcatct
     8761 ttacaggcat tggggtaggt ttcggctttg ttaatgtctt ttacggtgat aaggccggtg
     8821 aggttaaagg catcatccac cactagcact ttttcaatcc ggtggcgatg cagcaggccg
     8881 cgcacctcat cggcggaggc gccctcttta accgtcacta gtttttcttt gggggtcatg
     8941 atggatgcca cggtagcatt gaggttggac tcaaagcgca catctcggcc ggtcactatg
     9001 cccaccaaat tgcccttttc catcacgggt acaccggaga tgcggtgctg gcgggtaagc
     9061 ttgattaaat cgttgatgga agcagaagac tcgatggtaa tgggggcgcg caccacaccg
     9121 gcttcgaatt ttttcactgc atggacttca gccgcctgct gctcgatgct catgctttta
     9181 tgaataatac cgatgccgcc ctcttgggcc agggcaatgg ctaggcggga ttcggtcacc
     9241 gtatccatgg cggcggagac caagggaata ttcaggctaa tcttgcgggt taaagaggtt
     9301 tttaggctca cgtctttggc ggttacttca gagtaaccgg ggataagcag cacatcatca
     9361 aacgttaacg cttcttgagc gatccgtaac atagccaaaa cctcacctag gggcgtcgaa
     9421 aataaaaccg gcgattatag gcggctgcgg gtaaaagctc aaaccttcag caggtggcca
     9481 gcctattcct gcgggtttgc atgggcggcg tactacag
//
LOCUS       DAOQGS010000097         9491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3393_length_9491_cov_4.640420, whole genome shotgun sequence.
ACCESSION   DAOQGS010000097 DAOQGS010000000
VERSION     DAOQGS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3393_length_9491_cov_4.640420"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..393
                     /gene="ccoM"
                     /locus_tag="PKZ52_05330"
     CDS             265..393
                     /gene="ccoM"
                     /locus_tag="PKZ52_05330"
                     /inference="COORDINATES: protein motif:HMM:NF041600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit CcoM"
                     /protein_id="HNG59227.1"
                     /translation="MYLDDVVVSGIIILVLTCVFLGGVGWFVRKHMIEDAKSSGEQ"
     gene            complement(448..1062)
                     /locus_tag="PKZ52_05335"
     CDS             complement(448..1062)
                     /locus_tag="PKZ52_05335"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNG59228.1"
                     /translation="MQQQLIAMLELQEAMNNKVHPHWREQGYAWHRAIWVESAELLEH
                     YGWKWWKKQTPDHDQVALELVDIWHFGLSMQLQSGAPLELVAEDLAKGLRAPSGPQEF
                     NAAVEHFVAKTLLRQGFPVAEFAQLLQLSQLPFVRLYSLYVGKNILNFFRQDHGYKEG
                     TYKKIWQGREDNEHLIDILTRIDVHATDYKEQIYAALKAIFITA"
     gene            1156..1692
                     /locus_tag="PKZ52_05340"
     CDS             1156..1692
                     /locus_tag="PKZ52_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNG59229.1"
                     /translation="MTRSATRLAKWSLLDHRLFYFVHRYSHSRLFYWWARLCSASGDG
                     LIYGAAAVGLWRLQMFNPLKVLALGFALERCVYLIAKPVFRRNRPAAALTHYKAFIQP
                     ADTFSFPSGHSSAAFLFATVWHIADAGYSFIAFIWAANIAFSRVMLGVHFISDVIAGA
                     IIGVLIGMGCYQLLEVQA"
     gene            1689..2795
                     /locus_tag="PKZ52_05345"
     CDS             1689..2795
                     /locus_tag="PKZ52_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNG59230.1"
                     /translation="MKILYGVQGTGNGHISRARALNRELKLLGAEIDFIFSGRNANDY
                     FALDEFPKRAIYHGLHLAYTGGKVDFVQTWQKNKLPLVYSEINALDTRSYDLVISDFE
                     PITAWAAKLQRTRCLTLGHQYAFNHAVPIADETLFSRQLLKAVAPSELALGLHWYHFD
                     QPILPPIIQNLSSSACERGYYLVYLPFEDRQDIIDFLEPLESHSFICFGPYRSVEKVS
                     NILFRPCSANDFHPALCGARGVISNAGFELTSEALQLGIKLLVKPLQGQMEQVSNARA
                     LVELGLGLAMSRLDPRQLHYWLNNFQPRKITYPNVARAIAQWINKGNITLEPNRHLSR
                     QEAQKTIARLAADLWSQTDFSATPWLISRRKNAA"
     gene            complement(2906..3718)
                     /locus_tag="PKZ52_05350"
     CDS             complement(2906..3718)
                     /locus_tag="PKZ52_05350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNG59231.1"
                     /translation="MIVDSHCHLDRLDLAAHQQTLPQLIANARERGVAGMLCVCISED
                     NKDAVLAIAQEFDSVVASVGAHPSDVKDKVHSVATLVEWASAPKVVALGETGLDYYYS
                     ADYAGIQQESFQNHLVAAAQLQLPVIIHTRQAQQDTLRLMRDYADFTSSGVMHCFTED
                     WAMAKAALDMNFYISISGIVTFKNASDLREVVKKIPLERLLIETDSPYLAPIPYRGKS
                     NIPQYVREVADFIADLKGIHKEELYKTTSANFLRLFNKSAAHMPELVAYVNS"
     gene            complement(3802..4155)
                     /locus_tag="PKZ52_05355"
     CDS             complement(3802..4155)
                     /locus_tag="PKZ52_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG59232.1"
                     /translation="MQALGGSARNGILSLTIRDKAVLYAAYMPFIKNGGLFIPTNKSY
                     SLGDEVFMLLNLMDEPEKIPVAGKVVWITPKGAQGNRAAGIGVQFNADSEAASKKIET
                     YLAGTLESDRPTHTM"
     gene            complement(4281..5246)
                     /gene="holB"
                     /locus_tag="PKZ52_05360"
     CDS             complement(4281..5246)
                     /gene="holB"
                     /locus_tag="PKZ52_05360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNG59233.1"
                     /translation="MVSYPTDVLPWLQPIWQQLTQMMQLNRLPAGLLLTAAEGVGAAE
                     FARAFARILLCEAPREMNSCGVCAGCLLLANHNHPDIFILSPEEDSKVIKIAQIRALN
                     EFIANTAQQSGRKVVVIDPADAMNINAANALLKNLEEPIGQTYFILVSVNPSRLLATI
                     KSRCTRIALPTPLWAEGLAWLNQQGIEAAEAKLASAHGAPLRVVKWQQEGYFDQCAAL
                     AGHLEQYLTARQPLSYIAKPIAQLGVALVLDQCMAWVHSALVQPFKPQTLAVASLAEL
                     DEKDLLRLYESLQQKKRLLLAGVNLNAQLVVEELLVELGSLFKLA"
     gene            5337..5975
                     /locus_tag="PKZ52_05365"
     CDS             5337..5975
                     /locus_tag="PKZ52_05365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNG59234.1"
                     /translation="MIDVIYQDEHLIAVNKPSGVLSVPGLVCEDNLLDRVLKQFPNSR
                     TVHRLDMNTSGLLLFAQSYPAQKALNQLFAKGHIKKRYKAVVAGLVEADAGEIHLPLI
                     ADWPNRPRQKIDWQVGKTSHTFFRTLKRCPITQSTLLDLFPITGRSHQLRVHCLGIGH
                     PILGDQLYHLEHSHLQAPRLMLHAEHISLQHPITSEPLSIHCPDEFSALSCA"
     gene            complement(5962..6588)
                     /gene="tmk"
                     /locus_tag="PKZ52_05370"
     CDS             complement(5962..6588)
                     /gene="tmk"
                     /locus_tag="PKZ52_05370"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNG59235.1"
                     /translation="MSGFFISLEGVEGVGKSTNLTFIADWLKQQAVDFILTREPGGTE
                     LAEAIRTLLLTPREEAMAVDTELLLVFAARAQHVAEKIRPALARGQTVISDRFVDATY
                     AYQGFGRGLPLATIESLHQLILKNFLPHLTIYLDLDVETGLARARQRGELDRFERETA
                     AFFERVRAGYLARVQQDPKRFAVIDASLPLEQVQANIAQVLANYFKRS"
     gene            complement(6585..7634)
                     /gene="mltG"
                     /locus_tag="PKZ52_05375"
     CDS             complement(6585..7634)
                     /gene="mltG"
                     /locus_tag="PKZ52_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNG59236.1"
                     /translation="MIKQVIKPILLLLLGLGLCAMGATFWALQWMQTPTETAAAPLVF
                     EINRGESTAQIAGRLAQAGRVDWPLVWRAYAHWVEAKSLQAGEYELPAQTSPLSLLHQ
                     LQRGEVIKYSVTFPEGITYKEFVDILKQQPKLRHEVASKPIEEHKKILQFSYESLDGW
                     FFPDTYAFLAGDSDKDILLQAHKKMQQSLERAWRSRAENLPYMSASEALIMASIIEKE
                     TGLASEREKIAGVFVRRLKNKMRLQTDPTVIYGLGDSYTGNLTQKDLQTPTPYNTYLI
                     DGLPPGPIANPGLGALQAALHPAEGDELYFVAKGDGSHEFSATLAEHNKAVEHYQRTN
                     RSEHYQSAPSGKAAK"
     gene            complement(7639..8508)
                     /gene="pabC"
                     /locus_tag="PKZ52_05380"
     CDS             complement(7639..8508)
                     /gene="pabC"
                     /locus_tag="PKZ52_05380"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNG59237.1"
                     /translation="MTAPSIQSDATPITWVNGVRCGQVAIDDRGLAYGDGLFETMRYQ
                     AGALPLLPGHLERLTASAQRLNIAVSEAQVMSELELVLNDLATSAEGPIGALKLIVTR
                     GAGVGYTPAPEGRPNYYWLYRRCIEAVAMARQGVALQVSPLRLSRSALLGGLKHLNRL
                     EYVLAAQEPLVAEPPAAASQWLLLDEHGSVIEALTHNIFWLRDGVLFTPQLSHSGVAG
                     VMRQWLLQRAATETRAVTIGTFNLADVLGADEVFICNSLRGIWPVVQIEQTHFNSGPI
                     TRHYQSAIDSLWQ"
     gene            complement(8525..9163)
                     /locus_tag="PKZ52_05385"
                     /pseudo
     CDS             complement(8525..9163)
                     /locus_tag="PKZ52_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
BASE COUNT         2264 a         2528 c         2382 g         2317 t
ORIGIN      
        1 ttttttggca aattcaacga cgccagagcg agtaaggtaa tttttcagag gttccacagc
       61 ttaaacgctt gaccctagtc aaaccccatc ctatggcgac ttctatactt caggtgtaga
      121 agtcgttttt gtttattagg cacatggttc ctgcttgaga gttggcccct gtgcgccttt
      181 agacattcac caataagcgc cttcatcgca tagccaatgt attcggcttt tcttgtttga
      241 taaatttaat tggagcgcgt gcacatgtat ttagacgatg tggttgtatc tggaattatt
      301 atcctggttt tgacctgtgt atttttaggc ggcgttggct ggtttgtgcg caaacatatg
      361 atcgaagatg caaaatcaag cggcgagcaa taatcgccgt tggtgtgcgg ccaatctgca
      421 gcacacgcac attcacattg ccctaaccta cgcagtgata aaaattgctt tgagcgctgc
      481 gtaaatttgc tccttataat ctgtagcatg tacgtcgatg cgcgttaaaa tatcgatcaa
      541 atgttcgttg tcttctctgc cctgccatat ttttttgtag gtaccttctt tataaccatg
      601 gtcctggcgg aaaaaattca gaatattttt acccacatat aagctatata accgcacaaa
      661 tggcagctgg ctcaactgca acaattgcgc aaattccgct acgggaaatc cctgtcgcaa
      721 taaagtcttc gccacaaaat gttcaaccgc cgcattaaat tcctggggcc cgctcggtgc
      781 gcgcaggcct ttggctaagt cctcggcaac caactccaac ggcgcccctg attgcaactg
      841 catactcaaa ccaaaatgcc aaatatccac taattccaag gctacttgat cgtgatctgg
      901 ggtttgtttt ttccaccatt tccagccgta atgctccaac aactccgcag actccaccca
      961 aattgcgcga tgccaggcat agccctgctc acgccaatga ggatgaacct tgttattcat
     1021 ggcctcttgt aactctaaca tcgcgattaa ttgctgctgc ataaaaccct cataagccta
     1081 aagtgcaatc tttgtgtcat aaatgtccta tagcttaaca aacaacccac ccatgcacca
     1141 aagattgagg ttgctatgac acggtcagcg acacggttag ccaagtggag cctgctagat
     1201 caccggctgt tttattttgt gcataggtac agccactccc ggctttttta ttggtgggca
     1261 cggctgtgtt cagctagcgg cgatggctta atttacggcg ccgctgcagt gggtttgtgg
     1321 cgtctgcaga tgtttaatcc acttaaagta ctggcattag ggtttgcgct tgagcggtgt
     1381 gtttacctaa ttgccaaacc cgtttttcgc cgcaatcgcc cagcggcggc acttactcac
     1441 tacaaggcat ttatccaacc ggcggataca tttagctttc cgtccggcca tagttctgca
     1501 gcatttttat ttgccactgt gtggcatatt gcggatgcag gttacagttt tatcgcgttt
     1561 atatgggcgg ctaatattgc attttccaga gtgatgctcg gtgtacattt tatttctgat
     1621 gttattgctg gggcgatcat tggtgtgtta atcggcatgg ggtgttatca attgcttgag
     1681 gtgcaagcat gaaaatactc tacggagtac aaggcactgg caatgggcat atctcccgcg
     1741 cgcgggcgct caaccgcgaa cttaagctgc taggcgccga aattgatttt atttttagcg
     1801 gccgcaatgc gaacgattat tttgcccttg atgaatttcc caaacgagct atttaccacg
     1861 gcttgcacct agcgtataca ggcggcaagg tggattttgt acaaacctgg cagaaaaata
     1921 aactgccatt ggtatacagc gaaataaatg cactggatac acgcagctac gatctcgtca
     1981 tctcggattt tgaacctatc acagcttggg ctgctaaatt acaacgcacc cggtgcctta
     2041 cattaggcca ccaatatgcg tttaatcatg ccgttcctat cgctgatgag actttattta
     2101 gccggcaatt gttaaaagcc gtggcccctt ctgagctggc tttgggcctg cactggtatc
     2161 attttgatca gcctattttg ccgccgatta ttcaaaacct cagctcgtcc gcctgcgagc
     2221 gcgggtatta tttggtatat ctgccgtttg aagatcgcca agatattatt gattttttag
     2281 agccgctgga atcacacagt tttatttgtt ttggccccta cagatcggta gaaaaagtta
     2341 gcaatatttt atttcggcct tgcagtgcaa acgattttca cccggcactg tgtggtgcgc
     2401 ggggcgtcat tagcaatgcg ggttttgagt tgaccagcga agcgttgcag ttgggtatta
     2461 aattattggt aaaacccctg caggggcaaa tggagcaggt ttccaatgct cgcgcactgg
     2521 ttgaactggg cttaggctta gccatgtcac gcttagaccc acggcaatta cactattggc
     2581 tcaacaactt tcagccgaga aaaattacct acccaaacgt ggctcgcgcc attgcccagt
     2641 ggatcaacaa gggtaatatc actctggagc ctaaccgaca cttaagccgc caagaagcgc
     2701 aaaaaacgat tgcgcggtta gccgccgatc tttggtcgca aacagatttt tcagcaacac
     2761 cctggcttat atcgcgccgt aaaaacgccg cctaatacca cccctccgcc ctcataaacg
     2821 caaagtatac tcgggttaag cctacactcg tgaatagcgt gcaaacgcaa aaaccaaaga
     2881 gaattctacg caggcataac ggcgtttagc tattcacata agccaccaat tcaggcatat
     2941 gggctgcgga cttattaaat aaccgcaaaa aattcgccga agttgttttg tacaactcct
     3001 ctttatgtat acctttaagg tcagcgataa aatcagccac ttcgcgcaca tattggggaa
     3061 tattggattt gcctcgatag ggaatgggcg ctaaataggg tgagtcagtt tcaattaata
     3121 gccgctccag ggggattttt tttaccactt cgcgcaggtc gctggcgttt ttaaaagtga
     3181 caatacccga aatggaaata taaaaattca tatccaaggc ggctttagcc attgcccaat
     3241 cttcggtaaa acagtgcatt acacctgaac tggtaaaatc cgcgtaatcg cgcattaatc
     3301 taagcgtatc ttgctgcgct tggcgggtat gaataataac cggcaattgt aattgcgccg
     3361 ccgcgactaa atgattttga aaactttcct gctgaatgcc ggcataatct gctgagtaat
     3421 aataatccag gccggtttcc cccaaggcga caaccttagg tgccgaagcc cattccacta
     3481 gggttgcaac actgtgcacc ttatctttta catccgaagg atgggccccc accgatgcca
     3541 ccactgagtc aaactcttgg gcgatggcga gtacagcatc tttattatct tcactaatac
     3601 acacgcacaa catgcctgcc acaccgcgct cacgggcgtt ggcaattaat tgcggcagtg
     3661 tttgctggtg agcggcaaga tctaaacgat ccaaatggca gtgggaatca acgatcataa
     3721 ttatctgcac cacaaaaaaa ggcgctaatt ttgcgccttt taatgaatga agtcgatgcc
     3781 gcgctaggcg gatttagcct atcacatggt gtgggtcggt cgatccgatt cgagagtacc
     3841 ggctaaatag gtttcaattt ttttcgacgc cgcttcgcta tcggcattaa attgcacacc
     3901 tatacctgcc gcccgattac cctgggcacc cttaggggta atccatacaa ccttacccgc
     3961 aacagggatt ttttcgggct catccatgag gtttagcaac ataaacacct catcccctag
     4021 ggaataagat ttattggtag ggataaataa accaccgttt ttaataaacg gcatataggc
     4081 cgcgtacaac acggccttgt cgcgaatggt taatgagagt atcccgttgc gggcgctacc
     4141 acccaatgct tgcatgtgag cctcgtattc tttttcaata attgccgaca ttcaacctgg
     4201 cgcgaaaacc gctgacacct tagcgcgggg ggttggttgt gtccaggtac aaaacaactg
     4261 gccgtgtcgc acttctaagg ttaggccagt ttgaacagac tacccaattc tactaataat
     4321 tcttctacca ctaactgagc atttagattc acaccggcga gcaataggcg ttttttctgc
     4381 tgcaggctct cgtacaggcg caacaggtct ttttcatcta actcagcaag ggacgccacc
     4441 gcgagggttt gcggcttaaa aggttgcacc agcgcgctgt gtacccaggc catgcactga
     4501 tccaacacta aagccacgcc caattgtgcg atgggtttgg cgatatacga gaggggttgc
     4561 cgggctgtta aatactgctc taaatgcccc gccagggcgg cacactggtc aaaatagccc
     4621 tcctgttgcc acttaaccac gcgcaggggc gcgccgtggg cgctggcaag ttttgcctct
     4681 gccgcctcaa tgccctgctg atttaaccaa gccaggccct cagcccaaag gggggtgggg
     4741 agcgcaatgc gggtacagcg gcttttaatg gtggcgagca gccgcgatgg gttgacgctc
     4801 accaaaataa aataagtttg gccgatgggt tcttctaggt ttttcagcag tgcgttggcc
     4861 gcgttgatat tcattgcatc ggccggatca atcaccacca ctttgcggcc gctttgttgc
     4921 gcggtattgg caataaactc attgagggcg cgaatttgcg caattttaat taccttggaa
     4981 tcctcctccg gcgagaggat aaaaatatcc gggtgattat ggttggcgag caacaaacaa
     5041 ccggcacata caccacagga gttcatctcc ctgggggctt cacacagcaa tatacgggca
     5101 aatgcccggg caaactccgc tgcccccacg ccttcagcgg cggtgagcaa taggcctgcg
     5161 ggcaggcgat tgagctgcat catctgggtt aactgctgcc aaatcggttg cagccagggc
     5221 aaaacatcgg ttgggtagga aaccatagtt atgtcatttg gaagtaaaac gcggaacgcg
     5281 ctgcgtagtt atcgcaggga aattttggga gaatagcgcc ttttaggcca tattcagtga
     5341 tagatgtcat ttaccaggac gaacacctca ttgcagtcaa taaaccctcc ggtgtattga
     5401 gtgtaccggg gctggtgtgt gaggataatc tgctggatcg ggtgctcaag caatttccca
     5461 acagccgcac cgtgcaccgc ttggatatga atacctcagg cctattgttg tttgcccaat
     5521 cctaccccgc gcaaaaagcc ctcaaccaac tttttgccaa gggccatatt aaaaagcgct
     5581 acaaagctgt ggtggcgggc ctagttgagg cggacgcggg tgaaattcat ctgccgctga
     5641 tcgccgattg gcccaaccgg cctcgccaaa aaattgattg gcaggtgggc aaaacctccc
     5701 acacgttttt ccgcaccctt aagcgctgcc ctattaccca atcaaccctg ctggatttat
     5761 tccccattac cggccgttcc caccaattac gggtgcactg tcttggcatc ggccacccga
     5821 ttttagggga tcagctgtat cacctggagc actcccacct acaggcaccg cgcctgatgc
     5881 tgcacgccga acacatatct ctgcaacacc cgattacctc agaacccctg agcattcatt
     5941 gcccagatga attttctgcg ctcagctgcg cttaaaataa ttcgccagca cttgggcaat
     6001 attggcttgc acctgctcaa ggggcaggct tgcgtctatt acggcaaaac gctttggatc
     6061 ctgctgtacg cgggctaagt aacccgcccg tacacgctca aaaaatgccg ccgtttcccg
     6121 ctcgaaccga tccagttcac cgcgctggcg ggcgcgagcg aggccggttt ctacatctaa
     6181 atccaaataa atcgttaaat gcggcaggaa gtttttaagg atcaactgat gtaaagactc
     6241 aatagtggcc agcggcaggc cgcgcccaaa gccttggtag gcgtaggtag catctacaaa
     6301 tcggtcggaa ataaccgtct gcccccgcgc caaagccggg cggattttct cagccacatg
     6361 ttgtgcccgc gccgcaaaca ccagcagcag ctcggtatct acggccatgg cttcctcacg
     6421 gggggtcagc aacagcgtgc gaatggcttc tgctaattca gtgccgccgg gttcacgggt
     6481 gagaataaaa tccaccgcct gttgtttaag ccaatcggca ataaaggtga ggttggtgga
     6541 tttccccacc ccttccaccc cttccaggct gataaaaaaa ccgctcattt ggcagctttc
     6601 ccgctggggg ccgattgata gtgttcggag cgattagtgc gctgatagtg ctccaccgct
     6661 ttgttgtgct cggctaaggt ggcggaaaac tcgtggctgc catcgccctt ggcgacaaaa
     6721 tacaactcgt cgccctcggc cggatgcaag gcggcctgca gcgcacccag gcctgggttg
     6781 gcgatggggc cgggtggcag gccgtcgatt aaataagtgt tgtagggcgt tggggtttgc
     6841 aaatcttttt gcgttaaatt gcccgtgtag ctatcgccca gaccataaat aaccgtaggg
     6901 tctgtttgca ggcgcatttt attttttaac ctgcgtacaa aaacgcccgc aattttttcc
     6961 cgctcactgg ccaggcccgt ttctttttca atgatggacg ccatgatgag ggcttcactg
     7021 gcgctcatgt agggcaaatt ttctgcccgc gaacgccagg cacgctctaa ggattgctgc
     7081 atttttttgt gggcctgcag gagaatgtct ttgtcagaat caccggctaa aaaggcatag
     7141 gtatccggga aaaaccaacc gtctaaactt tcataactaa actgcagaat tttcttgtgc
     7201 tcctcaatgg gtttactagc aacctcatgg cgcaatttcg gctgctgctt gagtatgtca
     7261 acaaattctt tataggtaat accctcgggg aatgtcacgg aatatttaat cacctcgccc
     7321 cgctgcaatt ggtgcaacag gcttaagggg ctggtttgag cgggcaactc atactcaccg
     7381 gcctgtaagg acttagcttc cacccaatgg gcataggcgc gccacaccaa cggccagtct
     7441 actcggcctg cttgcgctaa acgcccggca atttgcgcgg tggactcgcc gcggttaatt
     7501 tcaaaaacca gcggcgcggc agcggtttca gttggggttt gcatccattg cagcgcccaa
     7561 aaggtggcac ccatggcgca caggccaagg cccagcagca ggagcaaaat gggtttaatc
     7621 acttgtttaa tcatcgggtt actgccatag ggagtcgatt gcgctttgat agtggcgggt
     7681 gatcggcccc gagttaaagt gggtctgctc aatctgcaca accggccata tgccgcgcag
     7741 gctgttgcag ataaatacct catcggcgcc gagcacatct gcaaggttaa atgtaccgat
     7801 agtcacagca cgggtctcgg tcgcagctcg ctgcagaagc cattgccgca tcacaccggc
     7861 aaccccgcta tggctcagct gcggcgtgaa caaaacacca tcgcgcagcc aaaaaatatt
     7921 gtgtgttagg gcttcaataa ccgacccgtg ctcatccaac agcagccatt ggctggctgc
     7981 agccgggggt tctgcaacga ggggctcctg ggccgccaac acatattcga gccggttaag
     8041 atgtttaagg ccgcccaaca atgcactgcg gcttaagcgc aggggggaaa cctgcaaggc
     8101 aaccccctgc cgagccatgg caacagcctc aatacaacgg cggtataacc agtaataatt
     8161 cggccgcccc tctggtgcag gcgtatagcc tacccctgca ccccgggtga ctatgagctt
     8221 gagcgcacca attgggccct cagcagatgt ggctagatca ttcagcacca gctcaagctc
     8281 gctcatcacc tgcgcttcag acacggcgat attaaggcgc tgggcgcttg cagtcaggcg
     8341 ctctaaatgc ccgggtaaga gcggtagcgc gcccgcttga taacgcatgg tttcaaatag
     8401 gccatcgcca taggcgagac cgcggtcgtc aatggcaacc tgcccgcaac gaaccccgtt
     8461 cacccaggta atcggcgttg catcactctg gatgctaggg gcagtcatgg cttcggtctc
     8521 gcatttatac gcggcggaaa actaaggttc cgttggtgcc accaaaccca aaggaattgc
     8581 tcaaggccac gttaaaagcg cgctcttggg cggtgtgggg gacaaaatta agatcgcagc
     8641 cctcatctgg ctcgtccaaa ttgatggtgg gcggcgctac ttggtgctgc agagccagca
     8701 cggtaatgat ggcctctaca gcgccagcag cccccagcaa atggccggtc atggattttg
     8761 tcgagcttac ggccagtgaa taggcgtgct caccaaacac cgacttaata gctgcgcact
     8821 cagccaaatc gccagcgccc gtggaggtgc cgtgagcatt gatataaccc accgaactac
     8881 tggctagacc tgcgtcgtgc agagcgttgg tgatggcaag ggcggcgccc tctccagtct
     8941 ccggcggcga ggtcatgtgg taggcatcac cgctggtgcc aaagcccacc aattctgcgt
     9001 aaatcttcgc gccgcgcgcc ttagccagtt cgtaattctc caacaccata acccctgcgc
     9061 catcgcccag cacaaaacca tcgcggtttt tatcccaggg gcggctggcc gcttgagggt
     9121 tgtcattgcg ggtagacagc gcccgcgccg caccgaagcc gccgggttcg agaaagtcgc
     9181 gctgcttgtc gatgatcagc agcgcggcgg tgcgcaggtc gcaggtgaac tcgtacggtt
     9241 ccgcgtcgat ccgcatatgg tgccctcccc aggggcgatg aaacagggtg ttgtaggcaa
     9301 tattgatgcc acgattatgt cgatgtgtta catggtttgc cacggaaatg gcatctggtc
     9361 gagggcccgt ggctgggccg atacgccgag aacgccggcc tggacatccg ccagcggttg
     9421 gaactggtgg cccgtgtctg cgatgccgtg cactacgcgc atcagaaggg aatcattcac
     9481 cgcgacctca a
//
LOCUS       DAOQGS010000098         9306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3486_length_9306_cov_4.929413, whole genome shotgun sequence.
ACCESSION   DAOQGS010000098 DAOQGS010000000
VERSION     DAOQGS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3486_length_9306_cov_4.929413"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..588)
                     /locus_tag="PKZ52_05390"
     CDS             complement(82..588)
                     /locus_tag="PKZ52_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNG59238.1"
                     /translation="MSVYRRNYVLGGTYFFTVNLLDRNSTLLVDYIDTLRTAVAEVKQ
                     RQPFLIDAWVVLPEHMHAVWTLPENDADYSGRWREIKKAFNRRIPPRELLSPARQKQK
                     ERGIWQRRFWEHTIRSDADYRHHVDYVHLNPLKHRLVKQVKDWPHSSFHHHVKKGTYP
                     ESWCGNTL"
     gene            complement(746..1123)
                     /locus_tag="PKZ52_05395"
     CDS             complement(746..1123)
                     /locus_tag="PKZ52_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59239.1"
                     /translation="MAVSIDEISTLEAILNEKLESERMRFSLSVHFIKDRLNDPRNDP
                     AISITELQGIFNRLIAVHVGRLKTLKDRESFTVRCRASGIHIPCTMTHDRDKSGKVTK
                     TIITITIMRKRDFKSKDKIILTV"
     gene            complement(1126..1338)
                     /locus_tag="PKZ52_05400"
     CDS             complement(1126..1338)
                     /locus_tag="PKZ52_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59240.1"
                     /translation="MSIEDNYWHQIDEVTGVAIFVDSFDNKEFDVRIGTITDSEPAGT
                     ITASSDEELNAKLAELYNKFQQNKGN"
     gene            complement(1539..3242)
                     /locus_tag="PKZ52_05405"
     CDS             complement(1539..3242)
                     /locus_tag="PKZ52_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNG59241.1"
                     /translation="MGNFTDRDIKLLAKQIKEKKEYNRPFTMLTGAGCSKSAGIPLAD
                     ELVAEIHNKFGQECKYKLDENQIMDYGACMGCLSKNERRDLLSPYLNNSKINWAHIAI
                     ASLMHSGFVGRVITFNFDSILARACGLIGLYPATYDFAAAATVDTDYIAPQAIIHLHG
                     QGWGKSLLNSDQETSDHVENIRPLIRSVLQDSPLLVVGYSGLSDPVFSVISEEYSGRE
                     RLWWAGYSPNPNDSVKNLIENHKRLAQYLGGCDADDFLISLAKELKCFPPLLFKNPYS
                     HIINEIKPIMDFPLVERDSADLLTRLKHELAASETAFNRQANIPELLMNKQWTTAIEK
                     IDPVTPELQRYLAWAYTGLGGTLISDGKMQADISAIEAGIEKQKKALELIPDFYPALH
                     NIGEAYLQLGKFTKDPAHLEESIKIFNIAASKKPDKADTLCGLGCASSELGKIKNDET
                     LALFGINKIRDALKIEPDSHKILNNLGACQLNAFSLTKNNSFLDEAKITLEKAEKIDP
                     NGAYNLACLYSILNRKEECMNLLFRCKQAGTLPNRGWLISDLDLNNVKGDPRFAELIE
                     T"
     gene            complement(3700..4098)
                     /locus_tag="PKZ52_05410"
     CDS             complement(3700..4098)
                     /locus_tag="PKZ52_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNG59242.1"
                     /translation="MALFMLDTNMCIYLMKNQPAKVAQRFAECYVGDVVMSAITYAEL
                     HYGVAVAHNPQKELAHLAALIEDIEVAPFDQAAGLAYGPIRTATRESKKDHLDKLIAA
                     HAVALNVVLVTNNLKDFAKYPGLKAENWLL"
     gene            complement(4098..4334)
                     /gene="vapB"
                     /locus_tag="PKZ52_05415"
     CDS             complement(4098..4334)
                     /gene="vapB"
                     /locus_tag="PKZ52_05415"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNG59243.1"
                     /translation="MQTTKLFKNGNSQAVRIPADLAFERTDIELEIERVGQEIRIRPA
                     RRSLSGVMAKFAQFSPDVLAEGRGSQEQAARDDL"
     gene            complement(4439..5137)
                     /locus_tag="PKZ52_05420"
     CDS             complement(4439..5137)
                     /locus_tag="PKZ52_05420"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNG59244.1"
                     /translation="MQKPKIVVFTGAGVSAESGIPTFRDSNGLWHNHRIEDVASPEGW
                     RRNPRLVLDFYNQRRADIARAQPNAAHIAIAKLEAAFDVWVVTQNIDDLHERGGSRKI
                     IHLHGEITKARSSVDEEDIYPIGYADIKLGDTCRQHSQLRPHIVWFGEAVLFYAEAEK
                     IISAADKILVIGTSLSVFPAAYLLQAARADAQKVLVSPQMEAVPWGYEYIDKTAVGAV
                     PPLVEQWLAEAGCK"
     gene            5234..6379
                     /locus_tag="PKZ52_05425"
     CDS             5234..6379
                     /locus_tag="PKZ52_05425"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNG59245.1"
                     /translation="MPMPFPSDSVGLVTPQLISFSEPIALTCGRSLDSFELMVETYGT
                     LNASASNAVLICHALSGHHHAAGFHAAEDNKPGWWDAYIGPGKAIDTNRFFVVSLNNI
                     GGCHGSTGPASINAATGEPWGADFPPLRVRDWVQVQARLADRLGIGQWAAVVGGSLGG
                     MQAMRWSLQYPERVRHCVVVASALKLSAQNIAFNETARQAILSDPDFCDGFYLKHNKI
                     PAKGLSVARMIGHITYLSDDGMGQKFGRELRSGTFEHGVDEPVEFQIESYLRYQGTSF
                     SKIFDANTYILMTKALDYFDLAREYNDDAAEAFKHAQANFLVIAFTSDWRFAPARSRE
                     IVNALLKANKNVTYAEIDSPYGHDAFLVAKQPRYEQLFRAYMQRISV"
     gene            6379..6969
                     /gene="metW"
                     /locus_tag="PKZ52_05430"
     CDS             6379..6969
                     /gene="metW"
                     /locus_tag="PKZ52_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNG59246.1"
                     /translation="MRLDHAIIDRWIKPSARVLDLGCGDGTLLQRLQQSKNIRGYGLE
                     IDSANIQHCAEKGLNVIEQNIEEGLHNFEDKSFDIVIMSQTLQTMYYPDKILAEMLRV
                     GRECIVTFPNFGHYKARLQLLFRGRMPVSEFLPYEWYNTPNIHFCTLNDFEVMCQQLN
                     MTVLDRLVVTRNEILPLSKILPNLLGETGIYRVTKP"
     gene            7205..7894
                     /gene="bioD"
                     /locus_tag="PKZ52_05435"
     CDS             7205..7894
                     /gene="bioD"
                     /locus_tag="PKZ52_05435"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNG59247.1"
                     /translation="MKHQYFIAGTDTDVGKTLVTCALLQKAQQLKLSTLGLKPVAAGA
                     RVTDEGLRNSDAEALLALTTEPMDYRQINPICLPQPISPHLAAAEQKIRISLDRLVGL
                     VRGSLMTKVDVALVEGAGGWRVPINERDNLSALAVELKLPVILVVGLRLGCINHALLT
                     AEAIMRDGLVLAGWVGNQLAADVQALDGIMHTLNQRIAAPCLGQIPFLPEGDFAAAVE
                     HVRLPFERDNG"
     gene            8017..8334
                     /locus_tag="PKZ52_05440"
     CDS             8017..8334
                     /locus_tag="PKZ52_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59248.1"
                     /translation="MPCQSVAAYVFQQAARNSRPKVFDYTIAVKMAITAARSSKLCKI
                     ILFNLRPFMPKIRSAKTGENTWIFLFLQLAAVCTAECGILAAIINPALNAAGLITLLN
                     FTG"
     gene            8353..8736
                     /locus_tag="PKZ52_05445"
     CDS             8353..8736
                     /locus_tag="PKZ52_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNG59249.1"
                     /translation="MIIIYGIKEQLNPIKAKLSNTLQQCMQEVLGLPEDKRAHRFIPM
                     DKEDFYCPGGRSDAYTVIEINMMQGRTEETKKALIKTIFARFEVDLGIKPVDVEITIQ
                     EQPPHCWGFRGMTGDEAKLNYSINR"
     gene            complement(9044..>9306)
                     /locus_tag="PKZ52_05450"
     CDS             complement(9044..>9306)
                     /locus_tag="PKZ52_05450"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59250.1"
                     /translation="LYTRSADSTGASSCTGTCATNWPPLYALESSKSFGDFTVIKRPA
                     AEGETPSWQWAYQGKALYFYAQDTEVGQAKGISAAWPLAQPK"
BASE COUNT         2229 a         2141 c         2220 g         2716 t
ORIGIN      
        1 ccgcaaatag cttgatcagc tgcttgcggt ggtataggag agatggtatc tacgcgtgaa
       61 ttatttaatc caaaatgcgt attaaagggt attgccgcac caactttcag gataagtgcc
      121 ttttttaaca tggtgatgaa atgatgaatg cggccagtct tttacctgct tcaccagtcg
      181 atgttttaac ggatttaggt gcacatagtc tacatggtga cgataatctg catctgatcg
      241 aatggtgtgc tcccaaaagc gccgctgcca aattccgcgc tccttttgtt tttgccgtgc
      301 aggtgacaac agctcgcgtg gaggaattct gcggttaaag gcctttttaa tctcccgcca
      361 gcggccagaa taatcggcgt cgttctccgg cagagtccac acagcatgca tatgctcggg
      421 caagaccacc caagcatcaa tcaaaaacgg ctgccgctgt ttaacctctg ccaccgctgt
      481 gcgcaatgta tcaatgtaat ccaccaataa tgtgctattg cgatccaata ggttcacggt
      541 aaaaaaatac gtgccaccca atacataatt gcgccgatac accgacataa tatctccttg
      601 tgaataattt tatctctcgt aggttggaag agcgcagcga cttccgacat ttggtgctct
      661 tggcaggcat tacctttgaa tgtcggaagt cgctgcgctc ttccaaccta cgcctgaatt
      721 gtttaatcca agttgcgcgt aagcgctaaa ccgttaggat tattttatcc ttagatttaa
      781 agtctcgttt gcgcataatt gttatagtga tgattgtctt ggtgactttt cctgacttgt
      841 cacgatcatg cgtcatagtg cagggtatgt gtatgcccga tgccctacag cgtaccgtga
      901 atgattcacg gtctttcaga gtttttaatc tgccaacatg gaccgcgata agtcgattaa
      961 atataccctg caattcagta attgaaatgg ccggatcatt gcgcggatcg tttaagcgat
     1021 ctttaataaa atgaactgaa agagaaaagc gcatgcgttc gctctccagc ttttcattta
     1081 aaatcgcttc gagggtggaa atttcgtcga ttgaaacggc catgattaat tccctttgtt
     1141 ctgttgaaat ttattatata gctcggcaag tttcgcgttc agctcttcat ccgaagaggc
     1201 ggtgatagtg cctgcgggtt cggagtccgt aatcgtgcca atgcgcacat caaattcttt
     1261 gttgtcaaaa ctatcaacga aaatggccac cccagtaact tcgtcgattt ggtgccagta
     1321 gttgtcctct atagacattt caaatttcat ttaaattgct ccattttaaa attgcttttt
     1381 gaaccataat taaaaggctc gtaggttgga agagcgcagc gacttccgac attcggtgcc
     1441 cgcacccggc attacccttt aggtgtcgga agtcgctacg ctcttccaac ctacgcatga
     1501 attatttaat ccaagttacg cgtttggcac atgagaattt aagtttctat taattctgca
     1561 aatcgaggat cgcctttgac gttgttcaaa tccaaatcgc ttattaacca gcctctattc
     1621 gggagtgtgc ctgcttgctt gcatctaaat aataaattca tgcattcctc ctttctattc
     1681 agtatggaat aaaggcaggc tagattgtat gctccatttg gatctatttt ttctgctttc
     1741 tctagagtta tttttgcttc atctaaaaat gaattgtttt ttgttaagga aaacgcgttt
     1801 aattggcatg cccccaagtt gtttagtatt ttatggctat ctggttcaat ctttaacgca
     1861 tctcgaattt tattaattcc aaataaagct agcgtttcat cgttttttat tttacctaac
     1921 tctgagcttg cacatcctag tccgcataga gtgtcagctt tgtcgggttt tttggatgct
     1981 gctatattga aaatcttgat gctttcttca aggtgcgctg ggtcttttgt aaatttacct
     2041 aattgtaagt aggcttcgcc tatattgtgt aatgccggat agaaatctgg aataagctca
     2101 agggcttttt tttgtttttc aattcccgct tcaattgcag atatatcagc ttgcattttt
     2161 ccgtcagata taagcgtacc acctaaacct gtatatgccc aagctaggta tctttgaagc
     2221 tcaggcgtga ccgggtctat tttttctatt gcagtagtcc attgcttgtt catcaaaagt
     2281 tctggaatgt ttgcctggcg attgaatgcg gtttcagatg cggcaagttc atgttttaat
     2341 ctggtcagaa gatcagccga atctctttcg acaagcggaa aatccatgat tggtttgatt
     2401 tcgttaatga tatgagagta aggattttta aataatagag gaggaaaaca ttttagttct
     2461 tttgcaagcg aaattaaaaa atcatctgca tcacacccgc caaggtattg cgccagtctt
     2521 ttgtgatttt ctattagatt ttttactgaa tcattcgggt tgggtgaata tcccgcccac
     2581 cacaatctct ctctgccact atattcttcg gatataactg aaaaaactgg gtctgataat
     2641 ccactgtatc caaccaccaa taacggactg tcctgaagaa ctgaacgaat tagagggcgg
     2701 atgttttcta catgatcgct cgtttcctga tcagaattta atagactttt gccccagcct
     2761 tggccatgta aatgaataat tgcctgaggg gctatatagt cagtatctac tgtcgcagct
     2821 gctgcaaaat cgtaggtggc agggtaaagc ccaatcaggc cacaagctct cgcgagaata
     2881 gagtcgaaat tgaatgtaat aactctgcca acgaagcctg agtgcattaa gctggcgata
     2941 gctatatgcg cccaattaat ttttgaattg tttagataag gactgagtag atcgcgacgc
     3001 tcattcttgg acaagcatcc catgcatgcg ccataatcca ttatttggtt ttcatctaat
     3061 ttatatttgc actcctgacc aaatttattg tgtatctccg ccaccaattc gtcagccaaa
     3121 gggatgcctg ctgattttga gcagcctgct ccagtgagca tcgtgaacgg cctgttatat
     3181 tccttctttt ctttaatctg tttggccaat aatttaatgt ctcgatcagt aaaatttccc
     3241 ataatttaga gcccaactaa ataatatatt ttcattgttt tgcaatttgt ataaatttat
     3301 acctataaaa acccccagca actttggttg ctgatttaaa aagttattgc attagattct
     3361 ctcttcttgt ttccccgaac aacatgatgg acagagcttg atgttcaacg ccataatttt
     3421 acaatgggtc tatgggtatt ttttatgcat caacgtttcc aacatcctcc gtcctataca
     3481 acacataccc ctcataatag taacttacat cagttccttt cacataaatc tcacacctac
     3541 caacctcatc gctaagcgcc tgccgcaaca gcaccttaat tcaacatcct taaccaggct
     3601 gcattccatt gccgtcaaat aatcattttt atccaaccgc tgccaatcca ctttttcaaa
     3661 tctcagtggc ttttattata atcctgtgta tcccagccat cacagcagcc aattctcagc
     3721 ttttaaccca gggtatttag caaaatcttt taaattatta gtaaccaaca ctacatttaa
     3781 cgccaccgca tgggcggcaa ttaatttatc caaatgatct tttttactct cgcgggtggc
     3841 ggtgcgtatg gggccgtagg ctaggcctgc ggcttggtcg aagggagcta cttcaatatc
     3901 ttcaattaat gccgccaggt gggctaattc tttttgtggg ttgtgcgcca cggcaacgcc
     3961 gtagtgcagc tctgcatagg tgatggcgga cataactaca tcacctacat aacattcggc
     4021 aaagcgctgg gccactttgg ctggctggtt tttcataagg tagatacaca tattggtatc
     4081 cagcataaat aatgccatta cagatcgtcc ctggcggctt gttcttggct gccccggccc
     4141 tcggctaaaa catctgggga gaattgggcg aatttagcca tcactccaga gagcgagcgg
     4201 cgggctgggc ggatgcggat ctcctggcct acccgttcta tctccagttc tatatccgtg
     4261 cgctcaaagg caaggtcagc tgggatgcgc acggcttggg aattgccatt tttaaatagt
     4321 ttggtggttt gcatggcggg cctcacaagt ggatgtacag gtatttacat tagctcaatt
     4381 gttgatcagt gtaaatactt gtgtgtacgt gtcttgggcg ttgcggtatg ccaataaatt
     4441 acttacaacc cgcctctgct aaccattgct caaccaaagg tggcaccgcc cccaccgccg
     4501 tcttgtcaat atattcatac ccccagggca cagcttccat ctgcggagaa accaaaacct
     4561 tttgcgcatc tgctcgcgcc gcctgtaaca aataagccgc aggaaaaaca cttaacgaag
     4621 taccaatcac caatatttta tccgctgcac taattatttt ttccgcctcg gcataaaaca
     4681 aaaccgcttc accaaaccac acaatatgcg ggcgcagttg gctgtgttgg cggcaggtgt
     4741 caccgagttt tatgtcggcg taaccgatgg gataaatatc ctcttcatcc acactgctgc
     4801 gggctttggt aatttccccg tgcaggtgaa ttattttgcg gctgccgccg cgctcgtgca
     4861 ggtcgtcgat attttgggtt accacccata catcaaaggc ggcctctaat ttggctatgg
     4921 cgatgtgggc ggcgttgggt tgggcgcggg ctatatcggc ccggcgttga ttgtaaaaat
     4981 ccaacaccaa tcgcgggttg cgccgccagc cttctgggga ggccacgtct tcaatgcggt
     5041 ggttgtgcca caggccgttg ctgtcgcgga aggtggggat gccgctttcg gcgctgacgc
     5101 ctgcgccggt aaataccact atttttggct tttgcatgct taacctcgct gtttgctgtg
     5161 tgccgggggc ttgtctattg cccgtgaggg gcggcaaaac tacacttacc cacctttttg
     5221 cctaggccct tgattgccca tgccttttcc ttcggattct gtcggccttg taacgccgca
     5281 attaatcagt tttagcgagc ccatcgcgct cacctgcggg cgcagtttgg acagctttga
     5341 gttgatggtg gagacctatg gcaccttaaa cgctagtgcc agtaacgccg tgttaatttg
     5401 ccatgcgctc tcggggcacc accatgcggc ggggtttcat gccgctgagg ataacaaacc
     5461 cggttggtgg gatgcttata ttgggccggg taaagccata gataccaacc gattttttgt
     5521 ggtgtcgctg aataatatcg gcggttgcca cggctccacc gggcctgcct ctattaatgc
     5581 cgccaccggc gagccctggg gggcagattt tccgcccctg cgggtgcgcg attgggtgca
     5641 ggtgcaggcg cgtttggcgg atcgtttggg catcggccaa tgggccgccg tggtgggcgg
     5701 cagcctgggc ggtatgcagg ccatgcgttg gagtttgcag taccccgagc gggtgcgcca
     5761 ctgtgtagtg gtggcctcgg cgttaaaact cagtgcgcaa aatattgcct ttaatgaaac
     5821 cgcccgccag gccattttaa gtgacccgga tttttgcgac ggtttttatt taaagcacaa
     5881 taaaatcccg gctaagggtt taagtgtggc gcgcatgatt ggccatatta cttatttaag
     5941 tgatgatggc atggggcaaa aatttggccg cgaattgcgc tcgggcactt ttgagcacgg
     6001 tgtggatgag ccggtggaat ttcaaattga aagttatttg cgctaccaag gtacgagttt
     6061 tagcaaaata tttgatgcta atacttatat tctaatgacc aaggccttgg attattttga
     6121 tttggcgcgg gagtataacg atgatgcggc agaagccttc aaacacgccc aggcgaattt
     6181 tttggtaatt gcctttacca gcgattggcg ttttgctccg gcccgctcgc gggaaattgt
     6241 caatgcttta ttaaaagcca ataaaaacgt cacctacgcg gaaattgatt ccccctacgg
     6301 ccacgatgcg tttttagtgg ccaaacagcc ccgttatgaa caattgttcc gggcctatat
     6361 gcagaggata agtgtgtaat gcgtttagac cacgccatta ttgaccgctg gataaaaccc
     6421 agcgcccggg tgctggattt aggctgcggc gacggcacct tattgcagcg gttacagcag
     6481 agtaaaaata ttcgcggcta tgggctggaa attgacagcg ctaatatcca gcattgtgcg
     6541 gaaaagggct tgaatgttat cgagcaaaat attgaagagg gtttgcataa ttttgaggat
     6601 aaaagttttg acatagtgat tatgtcgcag accctgcaaa ccatgtatta cccggataaa
     6661 atcctcgccg aaatgctgcg ggtggggcgc gagtgtattg tcaccttccc gaattttggc
     6721 cattacaagg cgcgtttgca gttattattc agaggccgta tgccggtgtc ggaatttttg
     6781 ccctatgagt ggtacaacac gccgaatatt cacttttgta ccctgaacga ttttgaagtg
     6841 atgtgccagc aattaaatat gacggtgctg gatcgtttgg tggtgacccg caatgaaatt
     6901 ttgccgctct ctaaaatact gcctaattta ttaggtgaga cgggtattta ccgcgtcacc
     6961 aaaccctagc agggtaacta ttcagcctcg ttttatgtgg ctggaggtga tttgctcact
     7021 tgctggtaac tttaaactct gtgttctagt gcagtcacca cagtaatact ccagcgcagt
     7081 ggtgactaca ggttattgca gacaccactg ctccaccgcg cgttgattgt gcggatagac
     7141 aaaaaattta ccagaattcc ggcttaagta gagtttacca aacacgaggt gctgaatagt
     7201 tgccatgaaa caccagtatt ttattgccgg taccgatacc gacgtgggta aaactctagt
     7261 tacctgcgcc ctattacaaa aagcccagca acttaaattg tccacccttg gtttaaaacc
     7321 cgtggcggct ggtgcgcgcg taaccgatga gggcctgcgc aatagcgatg ccgaagcact
     7381 gttggcttta accacagaac ccatggatta ccggcaaata aaccctattt gtttaccgca
     7441 gcctatctcc ccccatttag ccgccgctga gcaaaaaatc agaatttccc tggatcgttt
     7501 agtgggtttg gtgcgcggca gtttaatgac caaagtggat gtggccttgg tggaaggcgc
     7561 gggcggttgg cgggtgccga ttaatgagcg ggataattta agtgcgctgg cggtggaatt
     7621 aaaactgccg gtaattttag tagtgggttt gcgcttaggc tgtattaacc atgcattatt
     7681 aacggcggag gccataatgc gcgatgggtt ggtgctggcc ggttgggtgg gtaatcagtt
     7741 agcggcggat gtacaggctt tagacggcat tatgcacact ctaaatcaac gcattgcggc
     7801 gccctgtttg gggcagatac cgtttttgcc ggagggtgat tttgctgcgg cggtggagca
     7861 tgtgcgcttg ccgtttgaac gtgataatgg gtgatttcgg ttgccaatac ccatactagc
     7921 agcttgttga aatactacct tgctagctct ggcggcgtta aatttggcga aaaaatcctc
     7981 atttactgcg tgtaaactcc ggttttttcg ccaaatttgc cttgccagag cgtcgccgcc
     8041 tacgtatttc aacaagctgc taggaattca cggcctaaag tttttgatta tacgattgcg
     8101 gtgaaaatgg cgattactgc tgcacgcagc agtaaattat gcaaaattat attgtttaac
     8161 ctccggcctt ttatgcctaa gattcgcagc gcaaaaactg gcgaaaatac gtggattttc
     8221 ttgtttttgc agctcgcggc ggtttgcacc gccgagtgcg gcatccttgc cgccattatt
     8281 aacccggcac taaatgccgc cgggttgata acacttttaa acttcaccgg ataaatttag
     8341 gaagacacag ccatgattat tatttacggc attaaagagc agttaaaccc cattaaagcc
     8401 aaactctcca acaccttgca acagtgtatg caggaggtgt tgggcctgcc ggaggataaa
     8461 cgggcgcacc ggtttatacc tatggataaa gaagattttt attgcccggg cgggcgctct
     8521 gatgcctata cggtaattga aattaatatg atgcaggggc gcacggagga gactaaaaaa
     8581 gccttgatta aaactatttt tgctcggttt gaggtggatt tgggcattaa gccggtggat
     8641 gtggaaatta ctatccagga gcagccgccc cactgttggg ggtttcgcgg tatgactggg
     8701 gatgaggcta aattaaatta ttcgataaat aggtaagtcc ttttacgctg agcatcctgc
     8761 gttaatccgt ctcctgttgg atcctttaaa aatccatttg caatccttgt gggagtttgt
     8821 gtttatccca aaacccctcc ccaaccctcc ccttggtaag gggagggagt aatagcggct
     8881 tcgcacgagt tgatccttag tgcaaaaatt tttgttcccg agccttgtgt tccgtgtttt
     8941 taaattcctt ttttgctcct tggtttttcc ttaatttctc cattgggtct tcatccttgg
     9001 ccgccttatt tcggctgcgg cgctttattt cggctgcggc gctttatttc ggctgcgcta
     9061 ggggccatgc cgcactgatg ccttttgcct ggcccacttc cgtatcctgg gcataaaaat
     9121 acagagcctt gccttggtag gcccattgcc agctgggggt ttcgccttcg gcggcggggc
     9181 gtttgataac ggtgaaatcg ccgaagcttt tgctgctttc aagggcgtag agaggcggcc
     9241 agttggtggc gcaggtgccg gtgcagctgg aggcgccagt gctatcggcg ctgcgggtgt
     9301 aaaggg
//
LOCUS       DAOQGS010000099         9275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3509_length_9275_cov_4.935575, whole genome shotgun sequence.
ACCESSION   DAOQGS010000099 DAOQGS010000000
VERSION     DAOQGS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_9275_cov_4.935575"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKZ52_05455"
     CDS             <1..1286
                     /locus_tag="PKZ52_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNG59251.1"
                     /translation="GLDPSKTYIRLPSFEKVRGDPVLYSHASRVLHLESNPMNARTLV
                     QRHGKREVWVNPPPIPLTTEEMDGVFGLPYARVPHPAYGNAKIPAYDMIKTSVNIMRG
                     CFGGCTFCSITEHEGRVIQSRSHESILKEIEEIRDKTPGFTGAISDLGGPTANMYMLN
                     CKDPMIHANCRRLSCVFPGICKNLVTDHSPTTSLYRKARQLPGIKKIAIASGLRYDLA
                     VEDPEYVKELVTHHVGGYLKIAPEHTEAGPLNKMMKPGMGAYDQFKALFEKYSKEAGK
                     KQYLIPYFIAAHPGCEDGDMINLAKWLKANNFRLDQVQTFYPSPMALATAMYYSGRDP
                     LKKITYKSARVFTPKGLEQRRFQKAMLRFHDEKHWPMLREYLRKIGRGDVIGSHPGAL
                     IPDDVRGHKIPIKPKARGNFKINVPPGLRRGKSKR"
     gene            complement(1458..2555)
                     /locus_tag="PKZ52_05460"
     CDS             complement(1458..2555)
                     /locus_tag="PKZ52_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNG59252.1"
                     /translation="MNTPFLRRLIGIAGLISLTAQAAPDPNFHIYLMLGQSNMEGAAP
                     IESQDRVANPRVQVMQGQNCSGVSTQYGQWREALPTLVRCPQNGIGLGPGDTFGRTMA
                     TLSPSDVTIGLVGGAYGGAKIEYFMKSCGSDCTPPYGGIAGAPNNGTSGYQWVLDMAK
                     KAQQRGVIKGFIFHQGESNSGQQDWPSKVNTYISDLRRDLGLDPAKTPFIAGELPYTG
                     CCSGHNTLVNRLPSVITNAYVVKADGGLQDKGDGLHWNSAAVREMGKRYANQMAKVAD
                     IKSASSSSSSSSSSSSSSSSSSSSSSSSSSSGRSSSSSSSSSSSSSSSSSSSSTSSSG
                     SNNTGEVAGSTDGWMLVVMLPLLARIRRPRA"
     gene            complement(2941..4041)
                     /locus_tag="PKZ52_05465"
     CDS             complement(2941..4041)
                     /locus_tag="PKZ52_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNG59253.1"
                     /translation="MKTTFKTLAAIVSLITLPLTVTAAPDPNFHIYLMVGQSNMEGQG
                     AIEAQDKTPPANLLVMHDDKNCTQDGKKYLEWRPAVPPLMRCFQTAHSFNNGGLSPGD
                     YFGREMLKASAPGIKIGLVGAAYQGQKIEYFMKNCNPCTASGANGSIPEANAYTWLIT
                     MAKEAQKTGVIKGIIFHQGESNTGDTSWPSKVNTLVTDIRKDLGIGNVPFIAGEMVPN
                     ACCVSHNAQVQKIPSVVTNGYYVSAQGATKRSDDNYHFDAAGQRLMGKRYAEKMIPLV
                     DLNLSGSSSSSSSSSSSSSSSSSSSSSSSSGRSSSSSSSSSSSSSSSSSSSSSSSSSS
                     GNNNTGEVAGSGDPWLLVFMLPLLARIRRART"
     gene            4506..5174
                     /locus_tag="PKZ52_05470"
     CDS             4506..5174
                     /locus_tag="PKZ52_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59254.1"
                     /translation="MSIFASIVAALKNTFVPGLVLQFFALIILLVYFFLPGAHVYFDF
                     FGALKNHYGFAYSFVATAVFGGLIPFVYLWLSGSLPKGPGVVAIGIFYGLFWGLKGVE
                     VDLFYRLQAVWFGSGNDFSTLVAKVAVDQFIYSAFWAAPTITLSYLWMAAGFNLKTWW
                     QQLDKQYWAQTLPTVIVSNWLVWIPAVSIIYAMPNQLQIPLFNLVLCFWVLLLAVLNK
                     RDAQ"
     gene            complement(5692..6888)
                     /locus_tag="PKZ52_05475"
     CDS             complement(5692..6888)
                     /locus_tag="PKZ52_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNG59255.1"
                     /translation="MMGWLLTSILLMVLFSLLTQFLLQDPGFVLIALGNSSLEMRFWP
                     ALLSWLVFWLLLSLCATLLRRLLRGLRGGGWRTHRVAGEARLHAAWLHFFEGEWARLL
                     KDSLRALKRKPDLIPSLLAAEALANLGEPIGEGGRIHGLLSEAKLQHPKQTLSIALVG
                     ARIATQQGSESAALFWLEQGRNQAPRSRALLKLYAQVLRQKGDWPAITALLPELAKNK
                     AMDEASLTELEETTLAALLCSAAQTGLEALQNAWAGLNKSQKNAPNLLLAYARGLQKH
                     NRGELAEPLIRKSLNRSFNAPLAALYGHLEGINPQQQLKEAQAWLQAYPGEISVLISL
                     AQLCTRLHYWGQARDYWEAAHQQAPCARSYLALAELYTKLGDKVRSQELYERGLTRLA
                     ELEANS"
     gene            complement(6885..7964)
                     /locus_tag="PKZ52_05480"
     CDS             complement(6885..7964)
                     /locus_tag="PKZ52_05480"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNG59256.1"
                     /translation="MNDSSEKPPIAPEPRRSATGYWVLLLVFILVLGAIAVGVANVRH
                     QLKSEIKELEHKLTDLGRDQSQRYDDVMEDLEKARYRIENNLNSHQGRLSDIDQRLEE
                     HQKRLQSLTSQSLDERLLTEALLLLKQSNQRLLLEKDVPGATTLLTEVEKMLSSATGL
                     NANSEFLALRQAINRDLTALKLLTPVDKEGLYLQLNSLIELARQLPQKPKFEEKASGV
                     EPVVTNSWRDWVSEAWLRFKHGLSNYVRLENRSDKIKPLLTEESSQLVELTLRLVLEE
                     AQVALLQGRPAIYTSSLNKAEALLSQYYWESASLSQYLATLQQLRAANLSQELPSLSE
                     SVTLLQRLTEQSSAPLVKPAEVSAP"
     gene            complement(7974..8765)
                     /locus_tag="PKZ52_05485"
     CDS             complement(7974..8765)
                     /locus_tag="PKZ52_05485"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNG59257.1"
                     /translation="MASSPWRIWATRPAAQNPAWIEQLQQLGFEVFDFPLLAINPLED
                     ADSQQAIKSLVLDFDQFDKVIFVSQNAVQHAFNWLHDYWPQLPVGIRYFAVGDKTARN
                     LNMQGIDAEVACGMTSEALLNLPALTDVWGQKILICRGRGGLPKLGESLHQRGARVRY
                     LELYERLIPADAQQLLQAQLPSLPARDLLVAFSGETLHNLVSLFEGHPSVPWANPLLV
                     PGKRVEELARQLGFEQVICAANASEGAMLKSLTEFCTHHHPQAQA"
     gene            complement(8755..>9275)
                     /locus_tag="PKZ52_05490"
     CDS             complement(8755..>9275)
                     /locus_tag="PKZ52_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNG59258.1"
                     /translation="RPDLTVAFLRGNVNTRLSKLDAGDYHAIILAAAGLIRLDFHERI
                     ASFITSEDSLPAGGQGAVGIECRTQDDDIHRLLAPLHHTLTAEQVLAERAVNRRLEGG
                     CQVPIGCFAVHEGDQLWLRGLVAEPDGSAIIRSEIRGPASTAEALGIALAEDLLERGA
                     QRILQAVYANGL"
BASE COUNT         1914 a         2266 c         2774 g         2321 t
ORIGIN      
        1 ctgggcttga cccgagtaaa acctatattc gcctgccgtc ttttgaaaaa gtgcgcggcg
       61 acccggtgct ttattcccac gcctcgcggg tgctgcactt ggagtcgaac cccatgaatg
      121 cgcgcaccct ggtgcagcgc cacggcaagc gggaggtgtg ggtaaacccg ccgccgattc
      181 cgctgactac tgaggagatg gatggtgtgt ttggcctgcc ctatgcgcgg gtgccgcacc
      241 cggcctatgg caatgccaaa atccctgcct atgacatgat caaaacctcc gtgaatatta
      301 tgcggggctg ttttggcggc tgtacctttt gctcgattac cgaacacgaa ggccgcgtga
      361 ttcaaagccg ctcccacgaa tctatcctca aagaaattga agagattcgc gataaaacgc
      421 cgggttttac cggggcaatc tctgatttgg gcggccctac ggccaatatg tatatgctca
      481 attgcaagga cccgatgatc cacgccaatt gccgccgcct ctcctgcgtg tttcctggta
      541 tctgtaaaaa cctggtgacc gaccactcgc ccaccaccag tttgtaccgc aaagcccgcc
      601 agttgcccgg tattaaaaaa atcgccatag cctccggcct gcgctatgac ctagcggttg
      661 aggacccgga atatgtgaaa gagctggtga ctcaccatgt gggcggttat ttaaaaattg
      721 cgccggagca cacagaagct gggccactga ataaaatgat gaagcccggc atgggcgcct
      781 acgatcaatt taaagcccta ttcgagaaat acagtaaaga agccggcaaa aaacagtatt
      841 tgattcctta cttcatcgcc gcccatccgg ggtgtgaaga cggcgatatg attaatttgg
      901 cgaagtggtt aaaagccaat aatttccgcc tagaccaggt gcaaaccttt tacccctcgc
      961 ccatggcgct ggctaccgct atgtattatt ccggccgcga cccgctgaaa aaaatcactt
     1021 ataaaagtgc ccgcgtgttt acacctaagg gcttggagca gcggcgtttt caaaaagcca
     1081 tgttgcgctt tcacgatgaa aaacactggc ctatgttgcg ggagtatctg cgcaagatcg
     1141 gccggggcga tgtgattggc agccatccgg gggcattaat tcccgatgat gtgcgcggcc
     1201 ataaaatacc gatcaaacct aaagcgcgcg gcaattttaa aattaatgtg ccgccggggt
     1261 tgcggcgggg caaatccaaa cgctgagcga acaattgtta ttgcaccgct taactgctcg
     1321 cttaacaaaa agacgtttgc acggtaaaag agaagggaat acgggattaa aaaagcaggg
     1381 cgggcctata agaaacaaaa ggctggctag ccctgtagag aaagcctggc cgcaagggcc
     1441 aggtgttaac cgccgaatta ggcgcgaggg cggcgaatcc gcgctagcag cggcagcatc
     1501 actaccaaca tccagccatc cgtgcttccc gctacttcac ccgtgttatt gctgccagag
     1561 gagcttgttg agcttgagct gctagaggag cttgagctgg aagaagagct tgatgagctt
     1621 gagctgctgc ggccagagct tgatgaactg gaggagctgc tggagcttga acttgacgat
     1681 gagcttgagc tgcttgaact ggaagatgaa ctagaggcgc ttttgatgtc ggcaactttc
     1741 gccatttggt tggcgtagcg tttgcccatt tcgcgcacgg cagctgagtt ccagtgcagt
     1801 ccatcgccct tgtcctgcag gccgccgtcg gctttcacca cataggcgtt ggtgatgacg
     1861 ctggggagcc ggttaaccaa ggtgttatgg cctgaacagc agccggtata aggcaattca
     1921 ccggcaataa agggggtttt ggctgggtcg aggcccaggt ctcggcgcag gtcgctgatg
     1981 taggtgttta ccttgctggg ccaatcttgt tggccgctat tgctctcgcc ttggtggaaa
     2041 ataaaccctt taatcacacc ccgttgctga gcttttttcg ccatatctag cacccattgg
     2101 tagccgctgg tgccgttgtt aggcgcaccg gcaataccgc cgtagggcgg ggtgcaatct
     2161 gagccacagc ttttcatgaa gtattcaatt ttagcgccgc cataggcgcc acctaccaga
     2221 ccgatggtga catcgctagg cgagagggtc gccatggtgc ggccgaaggt atcgcccggc
     2281 cccaagccaa taccattttg tgggcagcga accagtgtgg gcagcgcttc gcgccattgg
     2341 ccgtactgtg tggatacacc ggaacagttt tgcccctgca tcacctgcac ccgcgggttg
     2401 gccacacggt cttgggattc aatgggggcg gcgccttcca tattggactg cccgagcatg
     2461 aggtaaatgt gaaaattagg gtcgggtgcg gcctgggcgg tcaggctgat taaacctgca
     2521 atgccgatta gtcttctcaa gaatggtgta ttcataggga tcacttttta tcgaattatt
     2581 atggaaagtg tgctttgccc aagggcagcg gcgtgtagat ggtgtacgtc gggtttgcat
     2641 ccgctcccat aaaacattaa ttggtaagac aattcaatcg ggtcaatggc tgagcttgta
     2701 tatctgtgaa ccagctggtg gttggggcgt ggagtagtag aaagttgctg agtctgtccc
     2761 cggggctact accttttcct ctagtttgcg tgcgcccatg agcgtttttt aataacgggc
     2821 agtaaatttg taacgggagg caaaatacag ccgaggagtt gaatagcgag gcggggaaat
     2881 caagggggga gttgcggtgg gtgggtaaag gcctggcgca aatggccagg cccatagggt
     2941 ttaggtgcgg gcgcgtctaa tgcgggctaa caggggcagc atgaatacca atagccatgg
     3001 gtcgccacta ccggctactt caccagtatt gttgttgccg gatgagctgg agcttgagct
     3061 gctggaactg ctagagctgg atgatgagct tgaactgctg gacgaggagc ttgagctgcg
     3121 gcctgagctg gatgaactgc ttgatgaaga acttgagctg ctggagcttg aggatgagct
     3181 tgagctgctg gatgagccac ttaggtttaa atccaccagt gggatcattt tctcggcgta
     3241 gcgcttgccc attaaacgct ggcctgcggc gtcaaagtgg tagttatcgt cggagcgttt
     3301 agtggcgcct tgcgcggaaa cgtaatagcc attggtgacc acggaaggaa ttttttgtac
     3361 ctgggcattg tgtgaaacac agcaggcatt cggtaccatt tcaccggcga taaaaggcac
     3421 attgccaatg cccaaatctt tacgaatatc ggttaccagc gtgtttactt tgctcggcca
     3481 agaggtatcg ccggtattac tctcaccctg gtggaaaata atgcctttaa tcacaccggt
     3541 tttttgcgcc tctttggcca tggtaattaa ccaggtatag gcgttggctt cgggaataga
     3601 gccgttcgca ccggatgctg tacaggggtt acaatttttc atgaaatatt caattttttg
     3661 tccctggtag gctgcaccca ccaagccaat tttgataccc ggcgcactgg cttttaacat
     3721 ttctcggccg aaataatcac cggggcttaa accgccgttg ttaaatgagt gagcagtttg
     3781 gaagcagcgc attaatggcg gtacagctgg gcgccattcc aaatattttt taccgtcttg
     3841 agtacagttt ttatcgtcgt gcatgaccaa caggttggca ggtggggttt tatcctgtgc
     3901 ttcaatggcc ccctggcctt ccatgttaga ttggccaacc attaaataaa tatggaagtt
     3961 aggatcaggt gcggcggtta cagttaaggg gagcgtaatt agactgacaa ttgcagccag
     4021 tgttttaaat gtagttttca tagggcccac ttcgataagc gttattattg atgtaatgca
     4081 ttgcttgctt gaatgaaaaa acaagcgcag ttgtgtagct atttcgccgt ctagcatgta
     4141 agcgcagttt gtcttataag tccatgtgcg ccgttagctg ttttgcgtaa ctgtgaacca
     4201 aattaaaaat taatttggct aaattaaaat gattcgcaaa taagcgtttt aatggtcata
     4261 ccaattgttt tttgcggcgt tattaggggt tgattgagta ttaaagacta taagcctgcg
     4321 cgtaatttag gcttttacct tttgggtagt gccgtgggat ttttaaaaaa tccggcgcta
     4381 cagcaggggt gaaataataa ggatgtaaga gtggttggat actgttttta agtgcttttg
     4441 tgggttgccc ttatgagcct tttggcggcg tgcaatggtg atgtttactt gagtttgagc
     4501 ctgatatgtc tatttttgcg tctattgttg cggccttaaa aaataccttt gttcccggtc
     4561 tggtattgca gttttttgcg ttgattattt tgctggttta tttttttctg ccgggcgcgc
     4621 atgtctattt tgattttttt ggggcattaa aaaaccacta tgggtttgcc tattcttttg
     4681 tggcaacggc cgtgtttggc ggcttgattc catttgttta tttatggctg agcggctcct
     4741 tacccaaggg gcctggggtt gtagccatag gtatttttta cggtttgttt tgggggctga
     4801 aaggggttga ggtcgatctg ttttatcgtt tgcaggcggt ttggtttggt tctggcaacg
     4861 acttttcgac cttggttgcg aaagtggcgg tggatcagtt tatttacagc gcattttggg
     4921 ctgcgcctac catcaccctc agttatttgt ggatggcggc gggttttaat ctaaagactt
     4981 ggtggcaaca attggataaa cagtattggg cgcagacgct gccgacggta attgtctcca
     5041 actggttggt gtggattccg gccgtcagca ttatttatgc gatgccgaat cagctgcaaa
     5101 taccgctatt taatctggtg ttgtgttttt gggtgttgct gttggcggtg ttgaataaac
     5161 gggatgctca atgatcgtgt tatgtcgcgt ttgcttgttt agggcgagct atccagtacc
     5221 ttgttagtat ggctggatgt gattctcgct ccgatcttct gcgagccctc actacttatc
     5281 ccgtgtggta aatacaggtt attacagaca cgccctgaat acatccgtgt aggcttgtca
     5341 aaaaggtccc tctttttgac ggtgtggaat aacctgtatt caccactgcc gcactgcgaa
     5401 ttgataaacc taaaaacctc gctacgagtt caactgcggt ttttaggtga gggttttggg
     5461 ggtgtagtgg tgtgctgaac gcggtagtgc gtttgggcaa ataaaaaagc cctagaacag
     5521 ggttctaggg ccaagcacct tgggagttac atgcaatgaa tcagactcat ggggctcgcg
     5581 tctgtcgctt gggagttcaa cataagcgcg caagtgtgtg actgggggtg gggggagaag
     5641 tttcccggct tttagatttt tattcgcagc aatgcagatt tgtgttgtgc gtcacgaatt
     5701 ggcttcgagt tcggccagtc gagtcagccc acgttcatac aattcttggc tgcgtacctt
     5761 gtcgccaagt ttggtgtaca gctcggcaag tgctaggtaa ctgcgggcgc agggggcttg
     5821 ttggtgggcg gcctcccagt agtctcgcgc ctggccccag taatgcaggc gggtgcagag
     5881 ttgggcgagg ctgattaata cactgatttc accgggatat gcctgcagcc aggcctgagc
     5941 ttctttaagt tgttgttgcg ggttgatgcc ctcaagatgg ccgtaaaggg ctgctaaggg
     6001 ggcgttaaag ctgcggttga gtgatttgcg gataagcggt tcggccagtt ccccgcgatt
     6061 gtgtttttgc aggccgcggg cgtaggccag tagcaggttg ggggcatttt tttggctttt
     6121 attaagaccc gcccaggcat tttgcagggc ttcaagaccg gtttgagcgg cgctgcacag
     6181 gagggctgcc agggtggttt cctccagctc ggtaaggctc gcctcatcca tagccttgtt
     6241 tttggccagt tcaggcagta gggcggttat ggcgggccag tcgccttttt ggcgcagcac
     6301 ctgggcgtag agcttaagca gcgctcggct gcgcggcgct tgattgcggc cctgctccag
     6361 ccaaaacaag gccgcacttt cactgccctg ttgggtggcg atgcgcgcgc ccaccagtgc
     6421 gatggagagt gtttgtttgg ggtgttgcag ttttgcctcg ctgagcaggc cgtgaatgcg
     6481 gccgccttcg ccgatgggtt cacccaaatt ggccaacgcc tcggcggcga gcaggctggg
     6541 gatgagatcc ggcttgcgtt tgagggcgcg caggctgtct ttgagtaagc gcgcccactc
     6601 gccttcaaaa aaatgcagcc aggccgcgtg cagacgggct tcacccgcca ctcggtgggt
     6661 gcgccaaccg ccgccgcgta ggccccgcag cagtcttctc agcagcgtgg cgcacaggct
     6721 cagcagcagc caaaacacta accagctcaa tagcgcaggc cagaagcgca tctccaggct
     6781 gctgttgccc agggcgatca acacaaagcc cggatcctgc agcaggaact gggtgagcag
     6841 ggaaaacagc accatcagca ggatgctggt aagcagccat cccatcatgg tgcgcttacc
     6901 tcggcgggtt tcaccagggg cgcgctgctc tgttccgtga gccgctgcaa cagggtgacg
     6961 ctttcactta gcgagggcag ctcttggctg aggttggcgg cgcgcagctg ctgaagggtg
     7021 gccaggtact ggctgaggct cgcgctctcc cagtagtact ggctgagcag ggcttcggct
     7081 ttgttgaggc tgctggtgta gatggcgggg cggccttgca ggagcgccac ctgggcttct
     7141 tccagcacca ggcgcagggt cagttccacc agttggctgg attcttcggt gagcaggggt
     7201 ttgattttgt cgctgcgatt ttccaggcgc acatagttgc tcaggccgtg tttaaagcgc
     7261 agccaggctt cgctcaccca gtcgcgccag ctgttagtta ccaccggctc aacgccgctc
     7321 gccttctctt caaatttggg tttttgcggc agctggcggg ccagttcgat cagcgaattg
     7381 agctgcaaat acagcccctc tttatcgacc ggggtgagca gttttaatgc ggttaaatcc
     7441 cggttgatag cctggcgcag ggctaaaaat tcgctgttgg cgtttaagcc ggtggcggag
     7501 gagagcattt tctccacctc ggtgagtagg gtggtggcgc cgggtacgtc tttttccagc
     7561 agcagccgct ggttgctctg tttgagcagc agcagggcct cggtgagcag gcgctcatcc
     7621 aggctctggc tggtgaggga ttgcaggcgt ttttggtgtt cttctaggcg ctggtctata
     7681 tcgcttaagc ggccctggtg gctgttgaga ttgttttcga tgcggtagcg agccttttcc
     7741 aagtcctcca ttacatcgtc atagcgctgg ctctggtcgc ggcctaggtc ggtgagcttg
     7801 tgttcaagct ctttaatttc gcttttgagc tgatggcgca cattggccac gcccacggcg
     7861 atggcgccga gcaccaggat aaacaccagc agcaaaaccc aataacccgt ggcggagcgt
     7921 ctcggctctg gggcaatagg tggtttttca ctgctgtcat tcatggcgtg aacctaggct
     7981 tgggcttggg gatggtggtg ggtgcagaat tccgtgaggc ttttaagcat ggccccttcg
     8041 ctggcgttgg ccgcgcagat cacctgctca aaacccagct gccgggctag ctcttccacc
     8101 cgtttgccgg gtaccaaaag cggattggcc cagggcacgc tggggtggcc ctcgaacagg
     8161 ctgactaaat tgtgcagggt ttcgccacta aaggccacca gtagatcgcg ggcgggcagg
     8221 ctgggtagct gcgcttgcag aagctgctgg gcatccgccg ggatcaggcg ttcatacagc
     8281 tctaaataac gcacccgcgc gccgcgctga tgtaggcttt cgccgagctt gggcaggccg
     8341 ccgcggcccc ggcaaataag aattttttga ccccaaacgt ccgtgagggc gggcagattg
     8401 aggagcgcct cagaggtcat gccgcaggcc acttcggcgt caatgccctg catgttgagg
     8461 ttgcgggcag ttttatcgcc cacggcaaaa tagcggatgc cgacgggcag ttggggccag
     8521 tagtcgtgca gccaattaaa ggcgtgttgc acggcgtttt ggctgacaaa aatcaccttg
     8581 tcgaactgat caaaatccag caccaggctt ttaatcgctt gctggctgtc agcgtcttcc
     8641 agagggttaa tggccaggag gggaaaatcg aagacctcaa agcccagctg ctgcaactgt
     8701 tcaatccagg cgggattctg ggcggcgggc cgggtggccc agatacgcca tgggctagag
     8761 gccattggcg tagaccgcct gcaaaatccg ctgggcgccg cgctctagta ggtcttccgc
     8821 cagggctatg ccgagggctt cggcggtgct tgccgggccg cgaatttcgc tgcgaataat
     8881 ggcgctgccg tccggctcgg ccaccaggcc ccgcagccag agctgatcgc cctcatgcac
     8941 tgcaaaacag ccgatgggca cttggcagcc gccttctaaa cgacggttta cggcacgctc
     9001 ggcgagtact tgttcggcgg tgagtgtgtg gtgtaaaggg gcgagcaggc ggtggatatc
     9061 gtcatcctgg gtgcggcact cgatgcccac tgcgccctgg ccgccagcgg gcaagctgtc
     9121 ttcgctggtg ataaaactgg cgatgcgctc gtggaagtcg agccgaatca gcccggcggc
     9181 ggcgaggata atggcgtggt agtcgcctgc gtcgagctta ctcaagcggg tgttcacatt
     9241 gccgcgcaga aaggccactg tgaggtcggg ccggg
//
LOCUS       DAOQGS010000100         9265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3513_length_9265_cov_5.111944, whole genome shotgun sequence.
ACCESSION   DAOQGS010000100 DAOQGS010000000
VERSION     DAOQGS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_9265_cov_5.111944"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKZ52_05495"
     CDS             complement(<1..634)
                     /locus_tag="PKZ52_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNG59259.1"
                     /translation="MVKVRQDRPTTLDGKVDIVSWIDYLRDTAKLSSDAITDLQRACE
                     LAQALEQAPGDKTDGWTEAYSSFSAGLEMAEILAGLQLDKDSLVAAILYRSVRENKCT
                     LTAIDKSFGTTVATLIDGVRRMAAISNLTNHTGDKVLGQPSTDQSDNVRKMLVAMVDD
                     VRVALIKLAERTCAIRAVKKAPIEKRQRVAREVSEIYAPLAHRLGIGHIKW"
     gene            complement(969..1883)
                     /gene="cysM"
                     /locus_tag="PKZ52_05500"
     CDS             complement(969..1883)
                     /gene="cysM"
                     /locus_tag="PKZ52_05500"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNG59260.1"
                     /translation="MSSDFDANPYPTIEQFIGNTPLVRLQRLAGATNNHILLKLEGNN
                     PAGSVKDRPAYSMITQAELRGEIKPGDTLIEATSGNTGIALAMTAAIKGYKMILVMPD
                     HMTQERKDAMLAYGAELILVPKAASMEGARDLALSLQAQGRGKVLDQFGNADNPLAHY
                     TGTGPEIWQQTQGRITHFVSSMGTTGTIVGNSKYFKEKNPAIQIIGLQPAAGASIPGI
                     RKWHPDYLPGIYTDRNIDQIVEMSQARAEQTMRDLARIEGIFCGVSSGGAVAAALDIS
                     RSVENATIVAIICDRGDRYLSSGLFSQP"
     gene            2016..4757
                     /locus_tag="PKZ52_05505"
     CDS             2016..4757
                     /locus_tag="PKZ52_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG59261.1"
                     /translation="MLWASILPLGVLSCILLLADILALHTAQRHIYQNSFELAHRLLT
                     QFELLSDQPIDHKKFNQLADILLSDSEIVGLSLSDEKNQIVYQAGVASHPHNALAGKT
                     PRRNNPQNADENWLVQIPNGAYTWTLVVDDERIHVAFYQNLLFHLIIFGASLLILMFI
                     YVQTKAKFLGPIRTLTHYLLSLKLSPHHAIPDPNQLSFLNDCALLMNEVIENLNRAQD
                     DLRNSYEAALSDFKESLESVEIQNIEMDMARKEAIQAERVKAEFLMHASSDLLDPLRQ
                     CLGAIGQLSRTTMTAEQADYINNLETTLRGIIGLAQDIVDFSRLENGKFQLDVKNVQV
                     RQVVHEALSFYAPLAATKSNRVLSIIQPDVMEILLGDPRRLQQVLGNLINQALNHGRQ
                     GNIAIKVSVVRAETSAQLVKFSVINYSGGFDDDFVAHFKRLLESKHGDFAITQGTALG
                     LSIARNLVEKMGGTLGIDLEPADQPSIWFTARLQVPQSRLQQPATLAPLKGCHVLVID
                     EQPASRLELTTLLTSLGANFLEVTAMDDLVHQLPRWREFKPQIAVVDVLNRANQFDKE
                     ATLTTIAQCNELLGISCLVVTSSTTTGSIQKDVDPINAGIITRPLLPGKLHQALAHQI
                     SVTPILGAINSTPKVIPSLKPSFSHRLLVVDDNPSNTKLIAEFLKPFPLEVVTCSSGA
                     SALALCRERRFDLVFMDIQMPDADGFAVTAKIRELELGDTRTPIVALTAQNAAENKTK
                     YIVGGMDDYLSKPVSYEELVQLLSKWLGKKFTRPAAPPSAPPAPLPALEQAEVSHQPV
                     VVVQESLKLAKDNAALARDMLQMLIESLQQEPVDWAQLLADNNWGELQNAAHKLYGGA
                     CYCGVPALKEAAHALDRDLQLGQLDTIEHKVALLCREIQRLLDWSEDYDLDALFELV"
     gene            5160..5603
                     /locus_tag="PKZ52_05510"
     CDS             5160..5603
                     /locus_tag="PKZ52_05510"
                     /inference="COORDINATES: protein motif:HMM:NF021082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HNG59262.1"
                     /translation="MKHPVASPLTTLVQTLNDGIAVYKSAIAKMEHDDCKELFSELIA
                     CREFALDYLQPYVVFQTGAPELGHGFGTVIHHTYSQVQDNMNEDHDLSLLKQLEQVED
                     ETLRAMEITAQTSANVFVSSIIRDLMPRMRACRAQILGLEEKLAA"
     gene            6285..6641
                     /locus_tag="PKZ52_05515"
     CDS             6285..6641
                     /locus_tag="PKZ52_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59263.1"
                     /translation="MRLTAWQRFFFALVLLIFVGQTAAASIAICPKQAHHPAEHETPP
                     CHQTHVAVKTSAHGHHPLPDCCQQLGHCLLQGAAALIGTELTFTAVISTTAPNYGYRR
                     TPPHPQNYSLFRPPIA"
     gene            6818..8923
                     /locus_tag="PKZ52_05520"
     CDS             6818..8923
                     /locus_tag="PKZ52_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HNG59264.1"
                     /translation="MTTPIVTGPANGLDHAPSGQTEVSRRRFVLGASTSLALTSLPIS
                     RALQAEPAASNSPRVLSGTVFDLSIGYQAVNFTGQERLATTVNGQLPGPTLRWKEGER
                     VTLRVTNNLKEDSSIHWHGIILPSSMDGSPGFDSEPIKPGATFEYQFDVQQSGTYWYH
                     SHSGLQEQTGLLGAIVIEAKTPAPQAYDREMTVLLSDWSDEAPQVIYAKLKKRSDYYN
                     FRERTLADTWRDFKALGLAGSIKTRRMWNQMRMSERDIADVTGFTYTYLMNGVTPDTG
                     WMGLFKPGERLRLRIINGAAMSIFDVRIPGLQMTVVAADGQEIQPVSVDEFRIAPAET
                     YDVLITPAAEQAYCLFAQTIDRSGYARGTLTPDIKLKASVPAMDPPPNLGHRDMGMGG
                     MDHSGHDMGAMDHSSHDMSKMDTGAMNHASHDMGKMDASAMNHAGHDMGKMDTGAMNH
                     AGHDMGKMDASAMNHAGQDMSKMDKPAMDHSGHDMSKMDMSHMDHSGHDMGKMAAAKT
                     APLAKAGFGSNFDTTDAGMVVHAPSEFGPHVDMHAMSPQSGLADPGIGLREHQARYGR
                     RVLRYADLKSLHPTRDKREPSREIELHLTGNMHRYMWSINGINFMGAEPLLLTFGERV
                     RIILVNDTMMTHPFHLHGMWSELETGDPNYIPRKHTVIVQPGSKISYLVTADAKGRWA
                     YHCHLLYHMLGMMRTVKVV"
     gene            8927..>9265
                     /locus_tag="PKZ52_05525"
     CDS             8927..>9265
                     /locus_tag="PKZ52_05525"
                     /inference="COORDINATES: protein motif:HMM:NF017119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HNG59265.1"
                     /translation="MNIQFIPRNTTPLCKTLLVCALAWPLLSQAADHSAHMDEAKHDH
                     GDNPLLVYGAIDHLETVLDGGEEVYALDAQGWIGKDLDKLWLKTDLTQTAGTQEEAEV
                     QALYSRAVSAY"
BASE COUNT         2267 a         2601 c         2340 g         2057 t
ORIGIN      
        1 cccatttaat gtggccgata cctaaacggt gggccaaggg tgcgtaaatt tcggaaactt
       61 cgcgggccac tcgctggcgt ttttcgatgg gggctttttt aacggctcga atggcacagg
      121 tgcgctcagc caatttaatc aatgcaacgc gcacatcatc aaccatagcg accagcattt
      181 tgcgcacatt gtccgactgg tcggtgctcg gctggcctaa tactttatcg ccggtgtgat
      241 tggtaaggtt actgatggcc gccatgcggc gcacgccatc aattaatgtg gcaacggtgg
      301 taccgaagga tttatcaatg gcggtgagtg tgcatttatt ttcccgcaca ctgcgataca
      361 aaatagccgc cactaaactg tctttatcca attgcaagcc ggctaaaatt tccgccattt
      421 caaggccggc actaaatgaa gaataggctt ccgtccagcc gtcggtttta tcgccgggag
      481 cttgttctaa ggcctgagct aattcacagg cgcgctgcaa atcagtaatt gcatcgctgg
      541 agagcttggc ggtatcgcgc aggtaatcaa tccaagaaac aatatcaacc ttgccatcaa
      601 gggtggttgg cctatcttgt ctaactttta ccatatcaat aaaaactcaa ttaactgtaa
      661 agcccggcga gggtggctta agtgagtaaa cacaaaagcc ggggctctag agcgacggga
      721 taattatttg ttccttgcgc ggaaggccag tacgcatgtg atcatttcta ctgcgcaggc
      781 ggcgcccaat tcaggtgccg ccagagcaat acccggtggc gggctggcaa agtggtatat
      841 caatgggctg cctaggggcc attagatcac gggttgatat tattatagtg tgtcaaaagc
      901 acctgttagc aggagccgct tccccacctg ccataggcgt agatgaaccg ggcaatctag
      961 cggcggcatc agggctgaga gaacaggcca gaggacaaat agcggtcacc tcggtcgcag
     1021 ataatagcca cgattgtagc attttcaaca ctgcggctta tatccaatgc ggcagccaca
     1081 gcgccgccgg aagatacacc gcaaaaaatc ccttcaatgc gcgccagatc gcgcatggtt
     1141 tgctcggcgc gcgcctgact catctccacg atctggtcga tattgcgatc ggtgtatata
     1201 ccaggcaaat aatccgggtg ccatttgcga attccgggaa tactggcacc ggcagcaggc
     1261 tgcaagccaa ttatctggat ggcaggattt ttctccttaa aatatttact gttgcccaca
     1321 atggtgcctg tggtgcccat ggagctgaca aaatgggtaa tgcggccctg ggtttgctgc
     1381 caaatttccg ggcctgtgcc ggtgtagtgg gccaaggggt tatccgcatt gccgaattga
     1441 tctaacacct tgccgcgccc ctgtgcctgt agggacagag ccaaatcccg cgccccttcc
     1501 atgctggccg ccttgggcac cagaattaat tccgcgccat aggcgagcat ggcatctttg
     1561 cgctcctggg tcatatgatc gggcatcacc aaaatcattt tgtagccttt aattgccgcc
     1621 gtcatagcaa gcgcaatacc cgtgttgccg ctggtggcct caattaaggt atcgccgggt
     1681 tttatttcac cgcgcagctc cgcctgagta atcatggaat aagccgggcg atccttcacc
     1741 gagcctgctg gattattgcc ttctaatttc aacaaaatgt gattgttagt tgcgcccgcc
     1801 aagcgttgca aacgcaccaa gggtgtattg ccaataaact gttcaatggt ggggtaggga
     1861 ttagcatcga agtcgctgga cataaatcac tcgccaatag cgccaaaacg gttaaggatg
     1921 cggattctac gccattttat gctaaggtgg cgccgctctt ctaagaaatt taacgacaca
     1981 atatgaaccg cttcgagaca cactttggca aaaaaatgtt gtgggctagc attctgccct
     2041 tgggcgtgct gtcgtgtatt ttactcttgg ccgatattct cgcactacac acagcgcagc
     2101 ggcatatcta ccaaaactcc tttgaattgg cgcatcgttt acttacgcaa tttgaactct
     2161 taagtgatca acccatcgat cacaaaaaat tcaaccaatt ggcggatatt ctgctgagcg
     2221 attcggaaat tgtcggctta agcctcagtg atgagaaaaa ccaaatcgtc tatcaagcag
     2281 gagttgcctc ccaccctcat aatgcgcttg caggcaaaac accccgccgc aacaatccac
     2341 aaaacgcaga cgaaaattgg ctcgtccaaa ttcccaatgg cgcctatacc tggacgctcg
     2401 tcgtcgatga tgagcgcatt cacgtggcgt tttatcaaaa ccttctcttc catttaatta
     2461 tttttggcgc ttctctgctg atattaatgt ttatatatgt gcagactaag gccaaatttt
     2521 taggccctat ccgcaccctc acccactatt tattgtcgct gaagctctcg cctcaccacg
     2581 ccattcccga cccaaatcaa ttaagttttc ttaacgattg cgcgctgctg atgaacgagg
     2641 tgattgaaaa tctcaatcgc gcccaagacg atttacgcaa ttcctatgag gcggccttaa
     2701 gtgattttaa agaatcatta gaatcggtcg aaattcaaaa tatcgaaatg gatatggccc
     2761 gcaaagaggc tattcaagcg gagcgggtaa aggctgaatt tttaatgcac gcaagttctg
     2821 atttactcga tcctctgcgc cagtgcttag gcgccatcgg ccagctctcc cgcacaacca
     2881 tgactgccga gcaggcagat tatattaata atttagagac caccctgcgg ggaattattg
     2941 gcctggccca ggatattgtg gatttttctc gcctggaaaa tggcaaattt caattggacg
     3001 tgaaaaatgt acaggtgcgc caagtggtgc atgaagcgct gagcttttac gcaccgcttg
     3061 cggccaccaa aagcaaccgg gtgttatcca ttattcaacc ggatgtaatg gaaattctgc
     3121 tgggcgatcc gcgtcgtttg caacaggtgc ttggcaacct cattaaccag gcgctcaacc
     3181 acgggcggca gggcaatatc gccatcaagg tcagtgttgt gcgcgcggaa acaagcgccc
     3241 agttggtaaa atttagcgtc attaattaca gcggcggctt tgatgatgat tttgtggcgc
     3301 attttaaacg cctgctggaa agcaaacacg gcgactttgc cattacccaa ggcacggcac
     3361 tgggcttatc gattgcgcgc aatttagtgg aaaaaatggg cggcacctta ggaatcgacc
     3421 ttgaacctgc cgatcagccc agcatctggt ttaccgctcg tttacaggtg cctcaatcac
     3481 gcttgcagca accggccacc ctagcgccat taaaaggctg ccatgtgctc gtaatcgatg
     3541 aacaaccggc cagccgttta gaattaacca cattgctcac cagcctcggg gcgaattttt
     3601 tagaagtgac cgccatggat gatctggtgc atcagctgcc ccgctggcgc gaattcaaac
     3661 cacaaatcgc cgtggttgat gtattaaata gggccaatca attcgataaa gaggccactt
     3721 taaccaccat cgcccagtgc aatgaactgc tgggtatatc ctgtttggtg gtaaccagca
     3781 gcacaaccac cggcagcatc caaaaggacg tagaccccat taatgccggc attattaccc
     3841 gccctttatt gccggggaaa ttgcaccaag ccctcgccca tcaaatttcc gtaacaccga
     3901 ttttaggggc gattaattca acccctaaag tcatccccag tttaaagccc agtttcagcc
     3961 accgcttatt ggtggtggac gacaatccgt ccaataccaa attaattgcc gaatttctca
     4021 aaccgtttcc actggaagta gtgacttgca gcagtggcgc cagcgccctc gccctctgcc
     4081 gcgagcggcg ttttgatctg gtgtttatgg atattcaaat gcccgatgcc gacggctttg
     4141 ccgtaaccgc caaaattcgc gaactagaac tgggcgacac ccgcacgccg attgtggcgc
     4201 tgacggcaca aaatgcggcg gagaataaaa ctaaatatat cgtcggcggc atggatgact
     4261 atttatccaa accggtcagc tacgaggagt tggtccagct gctgagcaaa tggttgggca
     4321 aaaaattcac ccgcccagcc gctcccccct cagcgccccc tgcaccactg cccgcgcttg
     4381 agcaggcgga agtcagccac caaccggtag tggtggtgca agaaagcctt aaattggcga
     4441 aagacaatgc cgccttggcc cgcgatatgt tgcagatgtt aattgaaagc ctgcagcagg
     4501 agccggtgga ttgggcccag ttactggccg ataacaactg gggcgaactg caaaatgccg
     4561 cccacaaact atacggcggc gcctgttatt gcggcgtacc cgcccttaaa gaggcggccc
     4621 atgcgctgga tcgggattta caactggggc agctggatac catcgaacac aaggttgccc
     4681 ttttgtgccg ggaaatccag cgcctactgg attggtcaga ggattatgac ctagatgcgc
     4741 tgttcgagct ggtgtaaatc ccgtcacaga cacgcgtttt gggctgttac caaacacccc
     4801 aaccattcac cgcccattac cataagtgtt aattaactag ccgatgcgta gcttttttac
     4861 acgcatcagt gaattaataa cgtgcaaatt ggtaacacat gcactgtttg gcggcgcatt
     4921 tttagccgcg caaccaaccg cccgcacaac caaaacccct gcgcccgcgc tgtttataac
     4981 caaaacactg ccctatgcgg tgtgttcagg gaaaagcatt gatatttcag gcgtttttta
     5041 gatcgaaacg aaatgtcggc aaatagcgcc cgagcatgga atgagacgtg cttctcactt
     5101 tttgcccgca tcacaagtga cgggtacttt ttcccgacct taagcagaag gaaaccccca
     5161 tgaaacatcc agtcgcaagc ccgctcacta cgctcgtgca aacactcaat gacggcatcg
     5221 ccgtgtataa atccgccatt gccaaaatgg aacacgatga ctgcaaggag ttgttcagcg
     5281 agttgattgc ctgccgcgag tttgccttag attatttgca accctatgtg gtttttcaaa
     5341 ccggtgcgcc agaactcggc cacggctttg gcacagtaat tcatcacacc tattctcaag
     5401 tgcaagacaa catgaacgag gaccatgact taagcctgct caaacaattg gagcaagtgg
     5461 aagatgaaac cctcagggcg atggaaataa ctgcgcaaac ctctgccaat gtatttgtca
     5521 gcagtattat tcgcgacctc atgccccgca tgcgcgcctg ccgcgcgcag atactcggcc
     5581 tggaagaaaa actcgccgcc tagcagcttg ttgaaatacg taagcggcga ctcaaaggca
     5641 cggcaaattt ggctgcgcgt gcaacgactg tttggacgca gggcgattcc atggatggag
     5701 ccgatttcag tcagtaaaat ttcatgcaga agattaattg cgtatctgct gagcctgcgt
     5761 ttaatgactg cagatacagc cttccatcgc tcacaacagc tgaatttagc tacccgcttt
     5821 ctgtgcagtt caatcctccc agtcatcaaa tatgtgcgca caggcgtagg ttttgtgttg
     5881 gtactcattt tgtgaggttt gacttgccgc aagctttgca tgggtttggt caacctaaaa
     5941 accgcagctg aactcgtagc gagggtgcct aaggcgccga atatgtgcgc ttttttatca
     6001 aaatttggcg aaagcagtag tcatctctac ggtgagtcaa attttgataa aaaagcgtgc
     6061 agattcaagc attaggcagc cgtagtagag ttcaactgcg gtttttaggt tcatcgccag
     6121 gcctaaaaat taaaaccgcc ccaccggttt tagctacaat cgcgcagaac ttttttattc
     6181 gctggcggtt tttactcatt aacctggagt gccgtaatta aactgcgtac ttttgcacct
     6241 aagccccgat tcattgccac cttttgaggt cgatccgttc acctatgcgt ttaaccgctt
     6301 ggcaacgatt tttctttgcg ctggtactgc tgatatttgt cggccagacc gccgctgcca
     6361 gtattgccat ctgccccaag caggcacacc acccagccga gcacgaaaca cccccatgcc
     6421 atcaaaccca tgttgctgtt aaaaccagcg cccatgggca ccacccgttg ccggactgct
     6481 gccagcaact gggccactgc ctgttgcagg gtgccgcagc cctaattggc acggaactga
     6541 cgtttacagc tgttatcagc accaccgccc ccaactacgg ctatcgccgc acaccgcccc
     6601 acccgcagaa ttacagcctc ttccgcccgc cgatcgccta accccagcct aaataatggg
     6661 ccgccaacgc ctcagcctga gctttaggcg gcttggcctg cgccaatact tgggatcaac
     6721 aacacataca ttcgcggccg aaggcgtagc cttgctccgg catgacggct cttacccatc
     6781 ccgcgctcaa tcaccctttg ctccaccagg tttttttatg acgacaccta ttgttactgg
     6841 gcctgcgaac ggccttgatc atgcgccctc gggccaaaca gaggtttccc gccgcaggtt
     6901 tgtgctcggc gcatccacct ctttggccct cacaagcctg cccatcagcc gcgcgttgca
     6961 agcggaaccg gcagcctcca atagccccag ggtgctcagc ggaactgttt ttgacttaag
     7021 catcggttat caagcggtta atttcaccgg ccaagagcgc cttgccacca cagtgaacgg
     7081 ccagttgccc ggccccaccc tgcgctggaa ggaaggcgag cgggtgacgc tgcgggtgac
     7141 caacaacctt aaagaagaca gctccatcca ctggcacggc attattctgc cttccagcat
     7201 ggacggctca ccgggttttg attccgagcc catcaaaccc ggcgcgacct ttgagtacca
     7261 attcgacgtg cagcaaagcg gtacctactg gtaccacagc cactcgggcc tgcaagagca
     7321 gaccggcctg cttggcgcca tagtgattga ggcgaaaacc ccggccccgc aggcctacga
     7381 ccgcgagatg acggtgctgc tgtccgactg gtccgacgag gcgccgcagg tgatttacgc
     7441 caagctgaaa aaacgcagcg attactacaa cttccgcgag cgcaccctgg cagatacctg
     7501 gcgcgacttt aaagccctcg ggcttgcagg cagcatcaaa acccgccgca tgtggaacca
     7561 gatgcgcatg agcgagcggg acatcgccga tgtcaccggc tttacgtaca cctatttgat
     7621 gaacggtgta acccccgata cgggctggat ggggctgttt aaaccaggcg aacggctgcg
     7681 cctgcggatc attaacggcg ccgccatgag tatttttgac gtgcgcattc ccggcttgca
     7741 gatgaccgta gtggcggcgg acgggcagga gattcaaccg gtgagcgtgg atgaatttcg
     7801 catcgccccg gcggaaacct acgatgtgtt aattaccccc gccgcagagc aggcctattg
     7861 cctgttcgcc cagaccatcg accgctcagg ctacgcccgc ggcacactca cccccgatat
     7921 caagctgaag gcaagcgtgc ccgccatgga cccgccgccc aatttaggcc atcgcgatat
     7981 gggcatgggt ggcatggatc actccgggca cgatatgggc gccatggatc actcaagtca
     8041 cgacatgagc aaaatggata cgggcgccat gaaccatgca agccacgaca tgggcaaaat
     8101 ggacgcgagc gccatgaatc atgctggcca cgacatgggc aaaatggata cgggcgctat
     8161 gaaccatgcg ggccacgaca tgggcaaaat ggacgcgagc gccatgaatc atgcgggcca
     8221 ggatatgagc aagatggata aacccgccat ggatcactcg ggccacgaca tgagcaaaat
     8281 ggacatgagc cacatggatc atagcggcca tgacatgggc aagatggcag ccgccaaaac
     8341 agcgccgctg gctaaagccg gttttggcag caactttgat actaccgatg cggggatggt
     8401 tgtgcatgcg cccagtgaat ttggccccca tgtggatatg catgcgatga gcccccagag
     8461 cggccttgcc gacccaggta tcggccttag ggagcaccaa gcgcgctatg gccgccgggt
     8521 attgcggtat gccgacttaa aaagcctgca ccccacccgc gataaacgcg agcccagccg
     8581 ggaaattgag ctgcacctca ccggcaatat gcaccgctac atgtggtcca tcaacggcat
     8641 caattttatg ggcgccgaac ccctgctgct gaccttcggc gagcgggtgc gcattatcct
     8701 ggtgaatgac accatgatga cccacccctt ccacctgcac gggatgtgga gcgagctgga
     8761 aaccggcgac ccgaattaca ttccccgcaa acacaccgtc atagtgcagc cgggctccaa
     8821 aattagctat ttggtgacgg ccgacgccaa gggccgctgg gcctaccact gccatctgct
     8881 ctaccacatg ctgggcatga tgcgcacagt taaggtggtg tagacgatga acattcaatt
     8941 tatccccaga aacactaccc ccctgtgtaa aacactgttg gtttgcgccc tagcctggcc
     9001 gctgctcagc caggccgcag accacagcgc ccacatggac gaggccaaac acgaccacgg
     9061 cgacaacccc ctgctggtat acggcgccat cgatcacctg gagacagtgc tggatggcgg
     9121 cgaggaggtg tacgccctgg atgcccaagg ctggatcggc aaagacttag acaagctctg
     9181 gctgaaaacc gacctgaccc agaccgccgg cacccaggag gaagccgaag tacaggcact
     9241 atacagccgc gcggtttcgg cctat
//
LOCUS       DAOQGS010000101         9080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3602_length_9080_cov_3.927091, whole genome shotgun sequence.
ACCESSION   DAOQGS010000101 DAOQGS010000000
VERSION     DAOQGS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_9080_cov_3.927091"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2813)
                     /locus_tag="PKZ52_05530"
     CDS             complement(156..2813)
                     /locus_tag="PKZ52_05530"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59266.1"
                     /translation="MSRRLGLAGSALYPSSMALSSFTMRFLFNSFITCCGVLLLAGCD
                     VSKGSGTSASQGSQDPDPVFVDSAVAYIERPLPKMNANIRPDALLSPADFNPGAHLIL
                     KARAQASAPETNISDKIFPPIKGAAGTLVPQLYDVKDPYPSRDGKKLLFALRAPQLAN
                     TPAELQPTWDIWEYDRTSDKARRILTSREEDDLGQDVQPAYLPDGRIVFSSNRQTLTR
                     QQVLDRNGAAYRASAEGTNEYSYNLHVVNADGTNLQQLTFNPAMDLNPRVLNDGQIVF
                     MRRDRLGGNDSISFYTIRPDGTELSFYYGYHSKNIGSQDKPATYFNPQVQDDGKILAS
                     LRPRSNTKYGGDLITLNTEQFYEREQSLSTASGSAQVSITQGAAYSQPLGTDEAPVAP
                     SPAGYFQAAYPLNDKEQRYLVSWSACRVLDPITKARIPCKNQPVELTEAEPLYSLWMY
                     NGATQTQLPVSIPPADSVMNLFNPIILADKPTLPLAAGNTSSSLRAENAGVLHIHQVN
                     DLGLNAYQGAKYLRVLGHVPLPKNDDLELVNGAFGVGGRGAGMFDILAYAPLETDGSI
                     KVKLPAQVPFTLELVDANGRRVGPRHQNWLSLGAGEIRDCKGCHVANNANAPHGRLAA
                     EPLPGTASIASSLPERVPTLGVAALSYSNISKAINALPANCQPLETTITWRSPANKDI
                     CLTKWEPGCASTINYLEHLQPLWLADRRSCDAQKAVTRDLTCTRCHDRTAPARMQEAG
                     ADAQTLADFAALNVQLDLRATPTDNTDFVTSYTQLFTSVNEQEYVQQDDGSYTSQEKL
                     YPTPVTITQADGTQVTEIQMLPRAIGARLDTAGARSTRASRFLARFNNAADANHYQGL
                     SVDEQFLISEWLDLGAQYYNEPSKAREEN"
     gene            3555..4103
                     /locus_tag="PKZ52_05535"
     CDS             3555..4103
                     /locus_tag="PKZ52_05535"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNG59267.1"
                     /translation="MTNNVYAAPVGNVVEEEQAFGEVNFFSPGSRVNRLRYWAHITLA
                     LYAFCVCIGLAGASAVISPELGIAIGGIAYIGFFVFSVIVGIQRLHDLNKSGWLWLLN
                     FIPLVNLYVVILVIFFPGTPGENRFGLPTPPSKTWHWVVALSFPVLAIVLGIVAAVAI
                     PQYQQFVNKAKAAQVMPVQDGN"
     gene            4632..5834
                     /locus_tag="PKZ52_05540"
     CDS             4632..5834
                     /locus_tag="PKZ52_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59268.1"
                     /translation="MKRPLRPLPVLVLGCASVLAAAPSYCDSWEQAKRIHDRLTGIPA
                     SETVLKEMKALIDQKQYTAAAYKAMDNDAFYRVTLKNWVAPWTNREQNQFIPLNDYIA
                     TVMGYALKDKDFRGILSDDALFVGQGVSPAYSLSSNAHYEALENSSLNFKDVLTEVTQ
                     SSLTGYTGAAAGIITSRAAAKSFFIAGTNRAMLRFTLMNHLCHDLEQLHDVTRVPDRI
                     RQDVSRSPGGDSRLFLTGCMGCHNGMDPLAQAFAYYNFEFDADNDTTGENGRLRYNAE
                     GVTDPKTKTRVVAKYHINNTTFPYGYVTTNDTWDNYWRAGQNASLGWGPGTGHGSGAA
                     ELGKELANSEAFSQCQAKKVFQQVCLRGPESKDDVAALANMVTTFKAEQYHLKSLFAT
                     AAVYCKGD"
     gene            5837..8383
                     /locus_tag="PKZ52_05545"
     CDS             5837..8383
                     /locus_tag="PKZ52_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNG59269.1"
                     /translation="MALYASMLKRISLCAGLALVVVFTQACSGGSGKRTQRIAPAPVI
                     KDEATVAQYSGKVPNQQVQRFIDNFWSHLANRTCGNCHGASGTAPRKFLDSQNIQNAY
                     DASLAVVDLTTPSKSALVAKMTATPAGHNCWAANSQVCADAIKLLIENWAAAEKGAPS
                     NATEIVLQVPKTLAKLDSLQFPTSTTSFAPLYNSILRKYCIGCHTPEALQAQQQQPYF
                     ASPDIAVSYAAAKPKINLDNPELSGFYQKVAGMKHNCWYNSDCTRSGDELLAALKAMA
                     ADIKANGPEVDPSAGPPASGVVDFANAQIVSAGGRFDANVIALYTFKEGQGDIAYDSS
                     GVAPDLNLRLSGDYQWEAAWGVKFNAGLNGVKPDGRAQGSVEASSKIAKQLRASGEFA
                     IEAWVIPGNVTQEGPARIVSLAGDDNHRNFMLGQHIYNYVFDNRSEGAKPTGERLQTA
                     DAAKVLQATQQHVVVNYSPTEGTNLFVNGEKVASAKPTETPALSPYDESFALVLGREV
                     SGNYGWAGTLRMLAIHKRPLTAQQVKTHYDVGVGQKFFMLFAVSELLGNSIPQAFVVF
                     EVQQFDSYSYLFNKPFFISLDPAAVPSNIHIKGVRIGVNGRESMIGQSFANLDTTISS
                     ANYNPKSGTPLSSIGTLVALEKGQGFDQFYLTFDAIGDKTSQRAPETHVVAVAPEAPD
                     KPTKSDIGVRTFGQINAALSVMTNVPESVVRDTYEQVKQQLPGSTAADGFVAAQQMGI
                     TQLAVKYCNVMAGNESYREALFPGLNMNQSHFDEPSKTLIKQQLMKALLSHGVKEDGS
                     SLVNQPSSAEMDQALQPLFATMDSCTNDCAKKTLTAACAASLGSAVMLLH"
     gene            8397..>9080
                     /locus_tag="PKZ52_05550"
     CDS             8397..>9080
                     /locus_tag="PKZ52_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspF"
                     /protein_id="HNG59270.1"
                     /translation="MKSKAPIGLNQPLKLADHPKPRTRRELISQGFQMGVGTLLGGSA
                     LSLLGASPAHAQLSADLLDALKTKGCGVNANASGSIPFICFDLAGGANIAGSNVLVGG
                     RGGQLDFLSTAGYRKLGLPGDQLPGQTNAANLTTGFFYDNSLGLAFHSDSALLKGIKK
                     RSTMATWSNTDGCAIAARSENDTGNNPHNPMYGINRAGANGSLLALVGSRASDSGGNS
                     MAPAMMINNA"
BASE COUNT         1952 a         2493 c         2605 g         2030 t
ORIGIN      
        1 gtgcggtgtg cagctccaaa acctggcctt taaaggtgag gaactcaccg tcggtgtcgc
       61 tccagcgggc ggtactggtg tccgccgctg caacacttaa gctggtaaaa agactgatgg
      121 cccaaagggt ggagagtaag cgcatagtga actaattaat tttcttcgcg ggccttggag
      181 ggttcattgt agtactgggc gcccagatcc agccactcgc ttatgaggaa ctgttcatca
      241 acactgagcc cttggtagtg attggcgtcc gccgcgttat taaaacgggc taagaaacgg
      301 ctggcgcggg ttgagcgggc gccagctgta tccagccgcg cgcctatggc tcggggcagc
      361 atttggattt cagtcacttg ggtgccgtct gcttgggtga tggtgacagg cgttgggtag
      421 agtttctctt gactggtgta gctgccgtcg tcctgttgca cgtattcctg ttcattgacg
      481 gaagtaaaca gctgggtgta ggaggtgacg aagtcggtat tgtcggttgg agttgcccgc
      541 aaatccaact gcacattgag ggcggcgaaa tccgccaggg tttgggcatc tgccccggcc
      601 tcttgcatgc gcgcaggggc ggtgcggtcg tggcagcggg tgcaggtgag gtctcgggta
      661 acggcttttt gcgcgtcgca gctgcggcgg tcggccagcc aaaggggttg caggtgctct
      721 aaataattaa tggtgctggc gcagccgggt tcccacttag tgaggcagat gtctttgttg
      781 gcgggggatc gccaggtgat ggtggtttcc aggggctggc agttggccgg tagggcgttg
      841 atcgctttgc tgatattgct gtaactgagg gcggcaaccc ccaatgttgg cacgcgctca
      901 ggcaggctcg acgcaatgct cgcggtgccg gggagcggtt cagccgcgag gcggccgtgg
      961 ggggcattgg cattattggc cacatggcag cctttgcagt cgcgaatttc tccggcccca
     1021 aggctcagcc aattctggtg gcgagggccg acccgacggc cattggcatc cactaattcc
     1081 aaggtaaacg gcacctgggc gggcagtttt accttaattg agccgtcggt ttccaagggg
     1141 gcataagcca ggatatcgaa catacccgcg ccgcgcccac ccacaccaaa ggcgccgttg
     1201 accagctcca aatcgtcgtt tttaggcagc ggcacatggc cgagtacccg tagatatttg
     1261 gcgccctggt aggcgttaag gcccagatcg ttcacctgat gtatatgcaa caccccggcg
     1321 ttttcggccc ttaagctgct gctggtattg cccgccgcca acggcagggt gggcttatcg
     1381 gcgaggataa tggggttgaa caggttcatc accgaatcgg cgggggggat gctgacgggt
     1441 aattgggttt gggtggcgcc gttgtacatc cacagggagt acaggggttc ggcttcggtg
     1501 agttccaccg gctgattttt acaggggatg cgcgctttgg tgatgggatc tagcacccgg
     1561 caggcgctcc agctgactaa gtagcgctgc tctttatcgt tcagcgggta ggccgcttgg
     1621 aaataaccgg cgggcgaggg tgctaccggg gcttcatcgg tgcctagggg ttggctgtac
     1681 gcggcgccct gggtaatcga tacctgagca ctgccactgg cggtgctaag gctctgctcg
     1741 cgctcgtaaa attgttcagt atttaaggtg atgagatcgc cgccgtattt agtattactg
     1801 cgcggacgca gggaggcaag aatcttgccg tcgtcctgca cctgggggtt gaagtaggtg
     1861 gcgggtttgt cttggctgcc gatgtttttg ctgtgatagc cgtagtaaaa actcagttca
     1921 gtgccatcgg gtctgatggt gtaaaagctg atgctgtcgt tgccgcctaa gcgatcccgg
     1981 cgcataaaca caatctggcc gtcgttgagc acgcgggggt tgagatccat cgccgggtta
     2041 aaggtgagct gttgcagatt ggtgccgtcg gcgttcacca catgcaggtt gtagctgtat
     2101 tcgttggtgc cctcggcgga ggcccggtag gcggcgccgt tgcggtcgag cacctgttgg
     2161 cgggtgaggg tttggcggtt ggagctaaac acaatgcggc catcgggcag gtaggccggt
     2221 tgcacatcct ggcccaggtc atcttcctcc cggctagtga gaatacggcg ggctttatcg
     2281 ctggtgcggt cgtactccca aatatcccag gtgggctgca actccgccgg tgtattagcc
     2341 agctgtgggg cgcgcagggc aaacagcagt tttttgccgt cccgggatgg gtaggggtct
     2401 tttacatcgt acagctgggg caccaaggtg cctgcggcgc ccttgatagg cgggaaaatt
     2461 ttatcgctga tgttggtttc cggcgctgag gcttgggctc gggctttgag aattaaatgg
     2521 gcgccagggt taaagtccgc cgggcttaaa agggcatcgg ggcggatatt ggcgttcatt
     2581 ttcggcagtg gccgctcgat ataggccacc gctgagtcca caaatactgg gtctgggtct
     2641 tgagatcctt ggcttgccga ggtgccggaa cccttgctca catcgcaacc ggccagcagc
     2701 aaaacaccgc agcaagtaat aaaggagtta aacaaaaatc gcatggtgaa tgaactcaac
     2761 gccattgagg aggggtaaag cgccgatcca gcaaggccca aacgacggct catgtcgtta
     2821 ctgcggcgct gtggctttta ccactataga aaatctttgc ctgccacaca ggataaatag
     2881 ccgatccgca aagcctgtca cgcctcgggt gggcggcttt ggtcggcagt ttaggcaaag
     2941 ctaacctgct tagtatcggc tgaaaagcag taattgcgcc aggcttgccc tgccgcttta
     3001 tttgtgagag ggggccgggt aggtaatttt gtaccctgtg atctgggtca gaattcgcaa
     3061 atattgggcg gcgaaggcgg tagggctgcg ggccaaaaat cttttggaag taaagccatt
     3121 accgacggca atccgcagcc tgcttaacca ggggcagatg cataattttt aggggagtaa
     3181 accgctgact aatgggttgc ctctgcattt gcgatttaat tagcccaatg ccttgggcgt
     3241 tggtgtgagg ctgcacacag ggcatctaaa gcctgtgaat tattctgctc ggcgcgcaag
     3301 tggttgctcg cgcgtaaatg caatgcttat agtggcggcg ggcaggagcg gcggtttatt
     3361 ttgcggtaat ttgttgtgtg ttacttcgcc actgatttgg cgcattttaa acaagggcga
     3421 tttttaaaaa tttccccatt gttttttgca tcccatgaag attaaacccg gctcaaatgg
     3481 ccgctcccct gcgcacgcac tttaatttta attaatcaaa attcgcccat agggtgatta
     3541 ttaaggagat atttatgaca aataacgttt atgcagcccc agtgggcaat gtggtggaag
     3601 aagaacaagc gtttggggag gtgaattttt tctcacctgg cagccgtgtt aatcgcttgc
     3661 gctattgggc gcatatcacg ctcgcgctct atgcattttg tgtctgtatc gggcttgcgg
     3721 gcgcaagtgc agttatttcc cctgaattgg gcattgctat cggcggcatc gcttatatag
     3781 ggttttttgt attttcggtc atagtcggta ttcagcggct acacgattta aataaatccg
     3841 gctggttatg gctgttaaat tttattccgt tggtgaacct gtacgtggtg atcctggtga
     3901 ttttttttcc cggtacaccg ggtgaaaacc gcttcggcct gcctacaccg cccagtaaaa
     3961 cctggcattg ggtagtggcc ttgtccttcc ctgtactggc cattgtattg ggtatagtcg
     4021 ccgccgttgc gattcctcag taccagcagt ttgtgaataa ggccaaagct gcccaagtga
     4081 tgccggtgca ggacggcaat taaccgaccg gttattgcag tgcccgcacc cgcttaggat
     4141 ttgcgggtgc gggtgttgat cgcagcctgt ttgccagacc aactccggca caatgtgact
     4201 gaaactgtcg tctttttttc ctgccgcgcc agaattccgg cctattttta atccgctgcc
     4261 ttgcaggctt aaaaaaataa tctagcaccc aggtgcggga ctgagccctg cggcgcttaa
     4321 caccgtccgt ttaactcatt tttccagcaa aagaagcggc ttttgacagc agtaaaaata
     4381 aaaccggtgt gtttactctg gcgatacagg ggattgcttg tgtgaataca tctggcggta
     4441 actgcagcgc agtggaattc agtcacaggt tgcacactta ggttttcaat tctccattgg
     4501 ctggaacaga acctgcctta aggccttgtt cgagtgctct gcgcgatgtg gttaacaacc
     4561 taaacagttg gccagcggcg ggcgggcgct tcaaccatac ttgtggatga acatgtcttt
     4621 gcggggtttt tatgaagcga ccattgcgcc ctttgcctgt attggtgctg ggctgtgcca
     4681 gtgtgctggc ggcggcaccc agttattgcg atagctggga acaggctaag cgtatccacg
     4741 atcgtctcac cggcatcccc gcctctgaga cggtgctcaa ggagatgaaa gccctcatcg
     4801 atcaaaagca atacaccgcg gccgcctaca aggccatgga taacgatgct ttttaccgcg
     4861 tcaccttaaa aaactgggtg gcgccctgga ccaaccggga gcaaaaccaa tttatccccc
     4921 tgaatgatta catcgccaca gtcatggggt atgccctgaa ggacaaagac tttcgcggca
     4981 tcttatcgga cgatgcgcta ttcgtaggcc agggggtttc gcccgcctac tcgttatcca
     5041 gcaatgccca ctacgaagcc ttggaaaaca gcagcctgaa ttttaaagac gtgctcaccg
     5101 aggtgacgca gtcttctcta accggttata ccggcgcggc ggcgggcata atcaccagcc
     5161 gggcggcggc caagagtttt tttatcgccg gcaccaatag ggccatgctg cgctttaccc
     5221 tcatgaacca cctctgtcac gaccttgagc aattacacga tgtaacccgc gtgcccgacc
     5281 gtattcgcca ggatgtctcc cgctcccccg gcggtgattc ccgcttgttc ctcaccggct
     5341 gcatgggctg ccacaatggt atggacccct tggcccaggc cttcgcctat tacaattttg
     5401 aattcgatgc cgataacgac accaccgggg agaacggccg actgcgctat aacgccgagg
     5461 gcgttaccga cccgaaaacc aaaacccgcg tggtggccaa gtaccacatt aataacacca
     5521 ccttccccta tggctatgtg accaccaacg acacctggga taactactgg cgagcgggcc
     5581 aaaacgccag cctcggctgg gggccaggca ccggccacgg cagtggagcg gctgagctgg
     5641 gtaaggagct ggccaattca gaggccttca gccagtgcca agcgaaaaag gtctttcagc
     5701 aggtgtgcct gcgggggcct gagagcaaag atgatgtggc ggcgctggcc aatatggtga
     5761 ctaccttcaa ggccgagcaa taccacctga aatctctgtt cgctaccgcc gccgtttatt
     5821 gcaaaggtga ttgatcatgg ccctttatgc ttcgatgtta aaacgaatta gcctgtgcgc
     5881 aggcctcgcc ttggtggtgg tttttaccca ggcctgctca ggcggcagcg gcaagcgcac
     5941 ccagcgcatc gcacctgcgc ccgtgattaa agacgaggcc acggtcgccc agtacagcgg
     6001 caaggtgccc aaccaacagg tgcagcggtt tattgataac ttctggtccc atctggctaa
     6061 ccgcacctgc ggcaactgcc acggcgcaag cggcacagcg ccgcgcaagt ttcttgatag
     6121 ccaaaatatt caaaacgcct acgacgcaag ccttgccgtg gtagatctca ccaccccgag
     6181 caaatctgcc ctggtagcaa aaatgaccgc caccccggca ggccataact gttgggccgc
     6241 caatagccag gtgtgtgccg atgccattaa attgctgatt gaaaactggg cggcggcgga
     6301 aaagggcgcc cctagcaatg ccaccgagat tgtcctgcag gtacccaaaa ccctcgccaa
     6361 gctggacagc ctgcagttcc ccacaagcac caccagcttt gcgccactct acaactccat
     6421 cctgcgtaaa tactgcattg gctgccacac accagaagcc ctacaggctc agcagcagca
     6481 accctatttc gcctccccag acatagctgt gtcttacgcg gcggccaagc ccaaaatcaa
     6541 tttggataac ccggaactct caggctttta ccaaaaagtg gcgggtatga agcacaactg
     6601 ctggtacaac agcgattgca cccgcagcgg cgatgagctg ttagcggccc ttaaagccat
     6661 ggcagcggac atcaaagcca atggtccgga agtggacccc agcgcgggcc cgcccgccag
     6721 cggtgtggtg gattttgcca atgcacaaat tgttagtgcc ggtggccgct ttgatgccaa
     6781 cgtcatcgcc ctctacacct ttaaagaggg gcagggcgat atagcctacg actccagcgg
     6841 cgtagcgccg gatttaaacc tgcgcctgtc cggtgattac caatgggaag cggcttgggg
     6901 ggttaaattc aacgcgggcc ttaatggtgt taaacccgat ggccgcgccc agggcagtgt
     6961 ggaagccagc agcaaaatcg ccaaacagct gcgcgcgtcc ggtgaatttg ccatcgaggc
     7021 ttgggtgatc ccaggcaacg taacccaaga gggcccggcg cggattgtca gccttgccgg
     7081 ggatgacaac caccgcaact ttatgctcgg ccagcacatt tataactatg tattcgataa
     7141 ccgcagcgaa ggcgccaaac ccactggcga gcgcctgcaa actgctgacg ctgccaaggt
     7201 gctgcaggcc acccaacagc acgtggtggt taactattcc cccaccgaag gcaccaacct
     7261 gttcgttaat ggtgagaagg tcgcctccgc caaacccact gaaactccgg ccttaagccc
     7321 ctatgatgaa agcttcgccc tggtgctggg gcgggaagtc tccggcaact acggctgggc
     7381 cggtaccctg cggatgctgg ccatccacaa acgccccctc acggctcaac aggtcaaaac
     7441 tcactacgac gtaggcgtag ggcagaagtt ttttatgctt tttgcagtat ccgagctact
     7501 gggcaatagc atcccgcaag cctttgtggt gtttgaagtg cagcagtttg atagctactc
     7561 ctacctcttt aacaagccgt tttttatcag cttagaccct gccgctgtgc ccagtaatat
     7621 tcatattaaa ggtgtgcgca ttggggtgaa cggccgcgaa tccatgatcg gccagtcttt
     7681 tgccaacctg gataccacca tctctagcgc caattacaac cctaaatctg gcaccccgct
     7741 ctccagcatc ggcaccttgg tggcgctgga aaagggccag gggtttgatc agttttacct
     7801 caccttcgat gccatcggcg ataaaaccag ccagcgcgca ccggaaaccc acgtggtggc
     7861 ggtagcgcca gaggcgccgg ataaacccac taaatctgac ataggtgtgc gcaccttcgg
     7921 ccagattaac gccgccctct ccgttatgac caatgtgccg gagtcggtcg tgcgcgacac
     7981 ctatgagcag gtaaaacaac agctgcccgg cagtaccgca gccgacggtt ttgtggccgc
     8041 ccagcagatg ggtattaccc aactggcggt gaaatactgc aacgtgatgg ccggtaacga
     8101 gagctaccgg gaggccctat tccccggctt gaatatgaac caaagccact ttgatgagcc
     8161 ctccaaaacc ctcatcaaac aacagctgat gaaagcgctg ctatcccatg gcgtgaagga
     8221 ggacggcagc agcttggtca atcaacccag cagcgctgag atggaccaag ccctgcagcc
     8281 cttatttgcc accatggaca gttgcactaa cgactgcgcg aaaaaaaccc ttaccgccgc
     8341 ctgcgctgcc agccttggca gcgcggtgat gctgttacat tgatcgggga gcctgtatga
     8401 aatccaaagc gccaattggc ctcaaccaac ccttaaaact cgccgatcac cccaaacccc
     8461 gcactcgccg cgagctgatt agccaagggt ttcaaatggg ggtaggcacc ctcttgggtg
     8521 gatcggccct cagcctgctg ggcgccagcc ccgcccatgc ccagctctcc gccgatttgc
     8581 tggacgcttt aaaaaccaaa ggctgcggcg taaacgccaa cgcctcaggc tccatcccgt
     8641 ttatttgttt tgacctagca ggtggcgcca atatcgcagg ctccaacgtg ttagtgggcg
     8701 gccgcggcgg ccagttagat tttttaagta ccgcgggcta ccgcaaattg ggcctgccag
     8761 gggatcaatt acccggtcaa accaatgccg ctaacctgac cactgggttc ttttacgaca
     8821 acagcctagg ccttgctttt cacagcgata gcgctctgct caagggcatt aaaaaacgct
     8881 caactatggc aacttggagc aataccgatg gttgcgccat agccgcccgc tcggaaaatg
     8941 acaccggcaa caacccccac aaccccatgt acggcatcaa ccgcgccggt gccaacggct
     9001 cgttgctggc cttagtgggc tcgcgggcgt ctgattccgg cggcaactcc atggcgcccg
     9061 ccatgatgat taacaacgcc
//
LOCUS       DAOQGS010000102         9027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3638_length_9027_cov_4.435243, whole genome shotgun sequence.
ACCESSION   DAOQGS010000102 DAOQGS010000000
VERSION     DAOQGS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3638_length_9027_cov_4.435243"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKZ52_05555"
     CDS             <1..913
                     /locus_tag="PKZ52_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG59271.1"
                     /translation="VRAILPLLLVIPLLALAIWAIVTYALGPLKSLTQALSQKSVRDF
                     SPLPRTRLPRELVPLVNAINGLLERLAQAFAREQRFSADAAHELRTPLAALKVHAANL
                     YASLKALGEQSQALELAERVQVSADRMGHLIQQILTLNRAADGAWSEHFKPTELLTLS
                     RQLCADYYPLADAKRQTLEIAAEGEFIVLGDPWALSILLSNLISNAIKYTPEGGQIRL
                     NLELQAHKVAWILEDSGPGIAVELRERVYDRFYRVGGDRHASDTLGCGLGLSIAQQIV
                     QLHGGSMAMSTSSLGGLAVTLMLPTPQ"
     gene            1226..1819
                     /locus_tag="PKZ52_05560"
     CDS             1226..1819
                     /locus_tag="PKZ52_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNG59272.1"
                     /translation="MIKPAIKYLGVFLSSVIVYAQALSAYSAEPACPEYLNQTLRKLH
                     SKDQLNLCSFYRPGAPILIVNTASHCGYTKQFKGLETLYQTYKDQGLVVLGFPSNTFK
                     QEEDNEMGTATICYENYGVTFPMFEKVDVRGSTAHPLFAYLGGKTKSPTWNFNKYLLA
                     GDKPTITYFGSNEEPLGGDLEAAIKKALAANKPTPTK"
     gene            complement(1834..3309)
                     /gene="thiI"
                     /locus_tag="PKZ52_05565"
     CDS             complement(1834..3309)
                     /gene="thiI"
                     /locus_tag="PKZ52_05565"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNG59273.1"
                     /translation="MHFILKLFPEITLKSTPVRNRMTRQLVDNLRLLIKRQFGAGKVV
                     QGWDQLEIILPSDEAADIRLMTHLLACTPGIANFALVRSFPLTDVHSIFEQTQSLWGP
                     SLIGKRFCVRVKRQGKHSFASTDVERYVGGGLNQHNATAGVDLKTPEIIIRIEVKDEQ
                     VFILEDKHQGLGGFPLGTQEDVLSLVSGGFDSTVASFQCIRRGLRTHYCFFNLGGKAH
                     ELGVKEIAFYLWHRYGSTHRVKFVTVPFADVVNEILQKVDPSCMGVILKRKMIQAANA
                     TASHAGLTALVTGEAIGQVSSQTLTNLKIIDAAAEHLVLRPLALMDKGSIIDIARAIG
                     AEHFAANIPEYCGVISVKPSAHLKAAKVLAQEQLMDDAILHKALADAQVQDIDKVMQD
                     VAAGSIAIERVHTPGAIDTIIDIRHPDEQENKPRDFSSTPSLIIPFYQLSQRVQSLDP
                     SRRYLLYCDRGVMSELQAQTLAQAGLRNVGVYRPDLNKNQA"
     gene            3694..5100
                     /gene="glnA"
                     /locus_tag="PKZ52_05570"
     CDS             3694..5100
                     /gene="glnA"
                     /locus_tag="PKZ52_05570"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNG59274.1"
                     /translation="MSESKTLQLIKQHEARWIDLRFTDFRGKEQHATFPASVVNDNFF
                     VDGKMFDGSSIAGWKGINDSDMILMPDDSTAVLDPFYDEPTINVRCNIVEPATMQGYD
                     RDPRSLGLRAEEYLKSTGLGDKALFGPEPEFFIFDSVHYASGMGGAFYKIKSEEAAWQ
                     SGDDVDGKHGHAPRVKGGYFPVAPIDSLHDIRGAMCNAMEAMGLEIEIHHHEVANAGQ
                     CEIGVGANTLVKKADEVQILKYCIHNVAHQYGKTATFMPKPLIGDNGSGMHVHQSFSL
                     NGVNQFAGEAYAGLSETALYYIGGIIKHAKALNAFCNASTNSYKRLVPGFEAPVMLAY
                     SARNRSASIRIPYVVGAKAKRIETRFPDPTANPYLCFASLLMAGIDGIQNKIHPGEAA
                     TKDLYDLEPEEEAQIPQVCASLEEALAALEADHEFLLKGGVFSKDFIDAYIELKKQDI
                     QRVNMTPHPVEFELYYSV"
     gene            5246..5809
                     /locus_tag="PKZ52_05575"
     CDS             5246..5809
                     /locus_tag="PKZ52_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNG59275.1"
                     /translation="MKRLQMLLVLTLCAVGVQAEIYKIKDAKGNVTFTDTPGDKPAET
                     VNLRPTMIAPSQPDSTSVPPVTTSIIEPGAPAEEAQPAKNESLYKAKILSPKEGAEIT
                     ADQRDLAVAVTVSPGLGETDEFQLTIDGQPYGETTRGNSFVIREVPRGEHNIRVNIVS
                     LEGKVLASSDSVRVFVLRTGVNSPARK"
     gene            6180..7256
                     /gene="glnL"
                     /locus_tag="PKZ52_05580"
     CDS             6180..7256
                     /gene="glnL"
                     /locus_tag="PKZ52_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNG59276.1"
                     /translation="MFDPAQYRQLLDNLITAVIVLDQRLNLCHLNLAAEMLLGVSGEQ
                     ALGKSIRYCFSEAEGTLDTLHESLRFNHHFTNRRVRWRLHNMHEITVDYSVSPNAEMG
                     VVILEVQPLDRLLRISREEALISSQETSRNLVRSLAHEIKNPLGGIRGAAQLLARELS
                     SQDLEEYTRIIIDETDRLRNLVDRMLGPNKPPERQLINVHEVLEHVAAVIRAESDKQI
                     AIARDYDPSIPELLADKEQLIQALLNIARNAMQALLENPSERSPKITLTTRIHRRYTI
                     GRRHHPLVVRMAIVDNGPGIPPELIEDIFFPMITGRAEGTGLGLAIAQKLINQHHGLI
                     ECNSQPGCTEFAVYLPLDDNYAET"
     gene            7243..8667
                     /gene="glnG"
                     /locus_tag="PKZ52_05585"
     CDS             7243..8667
                     /gene="glnG"
                     /locus_tag="PKZ52_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNG59277.1"
                     /translation="MPKPNLVWIIDDDRSIRWVLEKALQQSGINTDSFENGDRALARL
                     SSARPDAIISDVRMPGIDGLTLLSSIHKEYPNLPIIIMTAHSDLDSAVAAYQGGAFEY
                     LPKPFDVDEAVAVTQRALVHAKEQALEVAPAPAPMTTEIIGEAPAMQEVFRAIGRLSQ
                     SNITVLINGESGTGKELVARALHRHSPRREGPFIALNMAAIPRDLIESELFGHEKGAF
                     TGAAALRQGRFQQADGGTLFLDEIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVR
                     IIAATHQNLESLVEDNRFREDLFHRLNVIRIHLPKLAERREDIPRLAQHFLHRAALEL
                     NVEPKILTKEAQTYLSNLAWPGNVRQLENTCRWITVMAAGREVHVQDLPPELMERKDA
                     QAPAGDWEKALRYWADQCLARGQQDILSEAVPTFERALIETALKHTAGRKRDAANLLG
                     WGRNTLTRKLKELGMAGSDDADDD"
BASE COUNT         2201 a         2232 c         2337 g         2257 t
ORIGIN      
        1 tgtgcgggcg attttacctt tattactggt tattccatta ctggccttgg ccatttgggc
       61 catagtcacc tacgcacttg gccccctaaa aagccttact caagcgctca gccaaaaaag
      121 tgtgcgagat tttagccctc tgccgcgcac ccgcctgcct cgggagctgg ttcccctggt
      181 taacgcgatt aacggcctgc tggagcgttt ggcccaggcc ttcgcccgcg agcagcgttt
      241 ttccgctgat gccgcccacg agctgcgcac ccccttggcc gcccttaaag tgcacgcggc
      301 caatttgtat gcaagcctta aggcccttgg cgaacagtcg caggccctgg agctggctga
      361 gcgggtgcaa gtgagtgccg atcgcatggg gcatttaatt cagcagatac tcaccctcaa
      421 tcgcgccgcc gacggcgcct ggagcgagca ctttaagccc acagagcttt tgaccttgag
      481 ccggcaactc tgtgcggatt attacccgct tgccgatgcc aagcggcaga cccttgaaat
      541 agccgccgag ggtgaattta tagttctagg tgacccttgg gcgctgagta ttttattgag
      601 taacctcatt agtaatgcca ttaaatacac tccagaaggc ggccagattc gccttaacct
      661 tgaactacag gcacacaagg tcgcgtggat tttggaggat tcagggcctg ggattgcggt
      721 ggaattacgc gagcgggtgt acgaccgatt ttaccgggtg ggtggtgatc gccacgcgag
      781 cgacaccctc ggctgcggtt taggcctatc cattgctcag cagattgtgc agttacacgg
      841 cggcagtatg gccatgagta ccagcagctt aggtggactt gcggttaccc tgatgttgcc
      901 aacgccgcaa tgatgcctaa cgctgaatca tccgctgccg cgccgtcggg cgtaaaaatc
      961 ctaacggcca gtgggtgttt aatgtaccca caaatatgaa tgctgaatgc tttgggaagg
     1021 caatagtgaa atagccggtt aaacctgcgg attggagcat tccaaccaaa gatgttctga
     1081 ttaagtatga attcgtgact attttgtttg gcaactgcac acctaaatcc ttgtggttta
     1141 acagcttgtc ccttgtggca gttgcacagt attcccgcct tcagacttaa ccagaggttc
     1201 cttaacaaca aaatgagtgc gatttatgat aaagcctgca attaaatatt taggtgtttt
     1261 tttatcgagt gtgattgtgt atgcccaggc actaagtgcc tatagcgcag agcctgcctg
     1321 cccggaatat ttaaatcaga ctctgcgaaa attgcattcc aaggatcagc ttaatctatg
     1381 ttctttttat cgccccggcg cacctatttt aatcgtcaat accgccagcc actgcggtta
     1441 caccaaacaa tttaaaggct tggaaacgct ctatcaaacc tacaaagatc aaggtctggt
     1501 agttttgggt tttccctcta atacttttaa gcaagaggaa gataatgaaa tgggcaccgc
     1561 cactatttgt tatgaaaatt acggcgtaac ctttcctatg tttgaaaagg tcgatgtgcg
     1621 cggcagtact gcccacccat tatttgctta cttaggcggt aaaactaaat cacccacttg
     1681 gaatttcaat aaatatttat tggcaggcga taaacctacg atcacatatt tcggtagtaa
     1741 tgaagaaccc ttaggcgggg atttagaagc cgccattaaa aaagccttgg cggcaaacaa
     1801 acccacaccg acaaaataat tacggtgacc tggttaggct tgatttttgt tgagatcagg
     1861 ccgatacaca cccacattgc gcaggcctgc ttgggcgagc gtctgcgcct gcaattcact
     1921 catgacgccg cgatcgcagt agagcaaata gcggcggctg gggtcgaggg attgaactct
     1981 ttgcgacagc tggtaaaacg gaataattaa actgggggtg gatgaaaaat cccgcggttt
     2041 attttcctgc tcatcggggt ggcgaatatc gataatggtg tcgatagcgc ctggtgtatg
     2101 aacccgttca atggcaatac tgccagccgc aacatcctgc atgaccttgt ctatatcctg
     2161 cacctgggcg tcagccaggg ctttgtgcaa gatggcatca tccattaatt gttcttgggc
     2221 gagtactttg gcggctttta aatgggcgga gggtttaacc gagataacac cgcagtattc
     2281 aggaatattg gcggcaaagt gctcggcgcc aatggcgcgg gcaatgtcga taatactgcc
     2341 cttatccatc aacgccagtg ggcgtaatac cagatgttct gcagctgcat caataatctt
     2401 taaattggtt aaggtttggc tggaaacctg gccgatcgct tcgccggtta ccaaagcggt
     2461 aaggcctgcg tggctggcag tggcgttggc ggcttggatc attttgcgct tgaggatcac
     2521 gcccatgcag gagggatcaa ctttttgcag aatttcattg accacatcgg cgaaggggac
     2581 ggtgacaaat ttaacgcggt gggtagagcc gtagcgatgc cacaaataaa aggcaatttc
     2641 cttcacacca agttcatggg ctttgccgcc caaattaaaa aaacaatagt gtgttctaag
     2701 gccgcggcgg atgcattgaa aactcgccac ggtggagtca aagccgccgg atacgaggga
     2761 taacacatcc tcttgggtgc ccagcggaaa accacctaag ccttggtgtt tatcttctag
     2821 gataaatacc tgttcgtctt ttacttctat gcgaataata atttccggag ttttaagatc
     2881 gacccctgca gtggcgttgt gttgattcag tccgccacct acgtagcgct caacatcggt
     2941 agaggcaaat gagtgcttgc cttggcgttt aacccgcacg caaaagcgct tgccgatcaa
     3001 acttggcccc caaaggctct gggtctgctc aaaaatacta tgcacatcag tcagcgggaa
     3061 actgcgcacg agggcgaaat ttgcgatacc tggggtgcag gccagcaggt gagtcatcag
     3121 ccgtatgtct gctgcctcgt cgctcgggag gataatttca agctgatccc aaccctgcac
     3181 caccttgcct gcaccaaact ggcgcttaat cagcaagcgc aggttatcga ccagttgccg
     3241 cgtcatgcgg ttgcgaaccg gcgtactctt tagggttatc tcgggaaata gcttcaggat
     3301 aaagtgcata gggtgaacct aaaacagata agtgagcatt tttggtgcgt tcacgttttt
     3361 gatggctcca ttaatgatca ttttggtcgg atgaaaaagg gttaagttgc tgtgcagggc
     3421 attgcaaatg ctcagctgag cgcttttggc cattatggca tataacttgc gggcggcggg
     3481 tgcgacgaac cgcgcggcgg catgaacaaa aacctagctg cgtcgaggtt ggcaaagtgg
     3541 gggagttctt tcctggaaag tggcgccctt gcgtattcaa cgggaacctt ttagattacg
     3601 tgcccgaaaa tagagccata accaagtgcg gttattcacg ctgtttcttt cataacaatt
     3661 ccaatgtcct gaagccctac tggaggcgcc tacatgtcag aatctaagac tctgcagctt
     3721 atcaaacaac acgaagcccg ctggattgac ctgcgattta ccgattttcg tggcaaagag
     3781 cagcacgcta cctttccagc cagcgttgtc aacgacaact tttttgtgga cgggaaaatg
     3841 tttgatggct catctatcgc aggttggaaa ggtattaacg attcagacat gatcctgatg
     3901 cctgatgact caacagctgt gctggacccc ttctacgacg aacccaccat taacgtacgt
     3961 tgtaacattg ttgaacctgc cactatgcag ggctacgacc gcgacccacg ctctttgggt
     4021 ttgcgcgctg aagagtactt aaaatctacc ggtttgggcg ataaggctct gttcggcccc
     4081 gaacctgaat tcttcatttt cgacagcgtt cactacgcaa gcggtatggg gggcgccttc
     4141 tacaaaatta aatccgaaga ggccgcttgg cagtctggcg atgacgttga tggcaaacac
     4201 ggccacgcac cgcgcgttaa aggtggttac ttcccagtag cgcctattga ctctctgcat
     4261 gacattcgcg gtgctatgtg taacgccatg gaagctatgg gcttagaaat tgaaattcac
     4321 caccacgaag tggccaacgc tggccagtgt gaaattggtg ttggcgctaa cactctggtt
     4381 aaaaaagcag atgaagtgca aattctgaag tactgcatcc acaatgttgc gcaccagtac
     4441 ggcaaaaccg ccacctttat gcccaagccg ctgattggcg ataacggctc aggtatgcac
     4501 gtgcaccagt ccttctccct caatggtgtt aaccaatttg ccggtgaagc ctatgccggt
     4561 ttgagtgaaa ctgccctgta ttacatcggc ggcatcatca aacacgccaa agccctgaac
     4621 gccttctgta acgcctctac caactcgtac aagcgtttag tgccaggctt tgaagcccct
     4681 gtaatgctgg cctattcagc gcgcaaccgc tctgcttcca ttcgtattcc ttacgtggtg
     4741 ggtgctaaag ctaagcgtat cgaaactcgc ttccccgacc caacggctaa cccttacctg
     4801 tgttttgcct ctttgttgat ggcgggtatc gatggtatcc aaaacaaaat tcacccgggc
     4861 gaagcagcaa ctaaagacct gtacgatttg gagccagaag aggaagcaca aatcccgcag
     4921 gtgtgtgcct ctttggaaga agccctggca gctttggaag cggatcacga gttcctgctt
     4981 aaaggcggcg tgttctccaa agactttatt gacgcttaca tcgagttgaa gaaacaagat
     5041 attcagcgcg ttaatatgac tccacaccct gtagaattcg agctttacta cagcgtataa
     5101 gtcttaaggc tgaaccactt ggtagccccc gacagcctgc tgtcgggggc tttttatttt
     5161 taaggtacag ccttggggat gattattgcg ttttctgccg agtggggcac aatcaaaggg
     5221 cataacacat aaaacaaggt tgcttatgaa acgattgcag atgttgctgg tgttgacgct
     5281 gtgtgccgtg ggggtgcagg cggagattta taaaatcaaa gatgccaagg gcaatgtcac
     5341 gtttaccgat acccccggtg ataagcccgc tgagacggtt aatctgcgcc caaccatgat
     5401 cgccccttcc caacccgact caacaagcgt gcctccagtc actacgtcca tcatcgagcc
     5461 aggcgcccct gctgaggagg cgcaaccggc caaaaatgaa tcgctctaca aagcaaaaat
     5521 tctctctccg aaagaggggg cggaaattac cgccgatcag cgcgatcttg cagttgctgt
     5581 aactgtgtcg ccaggccttg gggagacgga tgaatttcag ttaaccatcg acggtcagcc
     5641 ctatggtgaa accacccgcg gcaatagttt tgttattcgg gaagtgccca gaggtgagca
     5701 caatattcgc gtcaatattg tcagcctgga gggcaaagtt ttggcttctt ccgattccgt
     5761 gcgtgtgttt gtgctgcgca ccggggtgaa cagccccgcg cgcaaataga aagacaccca
     5821 gcttcggacc tttagtctcg cgggagcgct atagcgctcc ccagttttat ccggctcgac
     5881 ctgtgcgata tcgggtcgcc gattgtggtt cactgcatta tattggtgcc ctcttataaa
     5941 cccgtttgtt ggccatagcg cctgcgctgc cagttaaaat ccttcacaca gcaacaactc
     6001 gcacaaaata acccgaatga gaagattgtt gccgctctaa ctccaattcc aacctcaccg
     6061 attagccact gccgccaagt gcccgcttta agtcgaaaca agctcaatac tggggcgggt
     6121 atttttcgtt gttggtttgg ttattgcttt accctggtac ctattttgga aaatgcacca
     6181 tgtttgaccc agcacagtat cgccagctgc tcgataacct tataacggcg gtcatagtgt
     6241 tggaccagcg gttaaatttg tgtcatctca accttgctgc agaaatgctg ttgggtgtat
     6301 ctggggagca agcccttggt aaatccattc gctactgttt ttcggaagcc gaaggcaccc
     6361 tcgatacgct gcacgaatcc ctgcgtttta atcaccattt tacgaatcgg cgggtgcgtt
     6421 ggcgcttgca caatatgcat gaaattaccg ttgattattc ggtaagcccc aatgctgaaa
     6481 tgggcgtggt aattttggaa gtacaaccat tggatcgcct gttgcgcatt agccgtgaag
     6541 aagccttgat ttcctctcaa gaaacatcgc gcaacttggt gcgctccctc gcccatgaaa
     6601 ttaaaaaccc tttggggggg attcgcggtg cagcgcaatt attagcgcgc gagttatcgt
     6661 cccaggattt agaagaatat acgcgcatta ttatcgatga aactgatcgg ctgcgaaatt
     6721 tagtcgatcg tatgctcggg cccaataaac cgccagagcg ccaactgatt aatgttcacg
     6781 aagtgttaga acatgtggcg gcggttattc gcgctgagtc cgataaacaa attgccatcg
     6841 cgcgggatta cgatccgagt attcctgaat tactggcgga caaagagcag ttaattcaag
     6901 cgctgttaaa tattgcccgc aatgccatgc aggcgctgct ggaaaatccc tccgagcgca
     6961 gccctaaaat taccctaaca acacgcatcc accgtcgcta taccatcggc cgcaggcacc
     7021 atcccctcgt ggtgcgaatg gcgattgtag acaatggccc tggtatccca cctgaattaa
     7081 tcgaggatat atttttcccg atgattacag ggcgcgctga aggaacaggc ttagggttgg
     7141 ccatagcgca aaaattaata aatcaacacc atgggttaat tgaatgtaat agccaacctg
     7201 gctgcactga atttgccgtt tatctgccat tggatgataa ctatgccgaa acctaatctc
     7261 gtctggataa ttgatgacga tcgctcaatc cgctgggtat tagaaaaagc cctgcaacaa
     7321 tcgggtatca ataccgacag ttttgaaaat ggtgatcgcg ccttggctcg tttatccagc
     7381 gcgcgcccgg atgccattat tagcgatgtg cgtatgccgg gtattgatgg gctcacgctg
     7441 ctctccagca ttcataagga atacccgaat ttacccatta ttatcatgac cgcgcactcg
     7501 gatttggatt ccgcagtggc ggcctatcag ggcggcgcat ttgaatattt gccaaaaccc
     7561 tttgatgtag atgaagctgt ggcggtaact cagcgcgcct tggtacacgc caaggagcag
     7621 gccctagagg tggcccctgc acctgcgccc atgaccacgg aaattatcgg tgaagcacct
     7681 gccatgcagg aggtgtttag agctattggc cgtttgtcgc aatcgaatat tacggtgctg
     7741 attaatggtg aatcgggtac cggtaaagag ttggtggcaa gagccctaca ccgccacagt
     7801 ccgcgccggg aagggccgtt tattgcgctg aatatggcgg cgattccgcg ggatttaatt
     7861 gagtctgaat tatttggcca tgaaaaaggc gcgtttaccg gcgcggccgc tttgcgccaa
     7921 gggcgttttc agcaggccga cggcggcaca ttatttttag atgaaattgg tgatatgcca
     7981 gcggatacac aaacccgttt attgcgtgtt ttggccgatg gagagtttta ccgcgtgggc
     8041 gggcacacgc cggttaaggt ggatgtacgt attattgccg ccactcacca aaatttagaa
     8101 agcctggtgg aggacaatcg ctttcgggaa gatttattcc accgcttaaa cgtgattcgc
     8161 atccatttgc ctaaattggc cgagcgccgc gaggatattc caaggcttgc gcaacatttt
     8221 ttacatcgcg ccgcattgga attaaatgtc gagccaaaaa ttcttactaa agaggcgcaa
     8281 acctatctga gtaatttggc gtggccgggt aatgtgcgcc agctggaaaa tacctgccgc
     8341 tggattacag tgatggcggc cgggcgggaa gtacacgtgc aggatttgcc gccggagctt
     8401 atggagcgca aagacgccca ggcacctgcg ggggattggg aaaaagccct acgctattgg
     8461 gctgaccaat gtttggcgcg gggccagcag gatattttgt cggaggctgt acccaccttt
     8521 gagcgcgcat tgattgaaac cgcacttaaa cataccgccg ggcgtaaacg ggatgccgct
     8581 aatttattgg gctgggggcg caatacgcta acgcgcaagc ttaaagaatt gggtatggca
     8641 ggttcggatg atgctgatga tgactaacag tgtcgcttag ctcttggggc ggctgagtta
     8701 gcagcttgtt gaaatgctgc cttgccagtg cgtcgccgcc taggtatttt aacaagctgt
     8761 taagggagct tttgcagatt tattctgtat ttaagttggt taataaaaaa ggcggagcat
     8821 aaaatgcccc gcctttttat gttggatgct tcgaatgaga agcgcgcaag ccgaggttat
     8881 tgcgccgcca tagtgcgggc gatgcggcct ttgcgtaggt cctcttcata taggtctaaa
     8941 tatttaaaaa taatattgcg cgccagtttt gtttcggagt tggttaaaaa cgccatgccg
     9001 atttgcaatt gacgaagacg gccacgc
//
LOCUS       DAOQGS010000103         8953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3673_length_8953_cov_3.714880, whole genome shotgun sequence.
ACCESSION   DAOQGS010000103 DAOQGS010000000
VERSION     DAOQGS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_8953_cov_3.714880"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKZ52_05590"
     CDS             <1..446
                     /locus_tag="PKZ52_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59278.1"
                     /translation="WKSHNEGVLPEDMLQYGIDISPLYSVRGKEYVPYFGYGRTGKGN
                     IFIETATGHGAVIYYYYSFPIAIDMYLEFEFVGAPDINGSPYLFQEQVRGRIKSITDS
                     LQLSLRDNHSCMGGVLGKWVIADSVKLLQPDPLTLPKHIPVDEIK"
     gene            533..1375
                     /locus_tag="PKZ52_05595"
     CDS             533..1375
                     /locus_tag="PKZ52_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59279.1"
                     /translation="MTIFTSFMAHAKDPTMYPRVIQLPDAELRFSMMEDFSNDMPAEP
                     LIEKIESANLSELKNITDRFVIGRRWWDLKPPGWFKKSWGSIQMTLMLGGAINKQGAK
                     LDFCTFSQADFMVFYHEALVEKWRKHNESITPENQIQFGVYVMSLFGAIGQEFIPHSG
                     FKQTGHNALLLESVAAQDANIYSYYSIPVGRDTFLEFEFIGAPDLNGSPYLFQAQADE
                     RINSIRQSVALKLDQNRSCVAGVLDKWVVADSVQLLKPDPSLLPKPAPSGTLEKFFPE
                     KKGP"
     gene            complement(1710..2150)
                     /locus_tag="PKZ52_05600"
     CDS             complement(1710..2150)
                     /locus_tag="PKZ52_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59280.1"
                     /translation="MRKRTLLSRIAVLNHGKKAYLEYLRNLTPQSALLVFALLAAYKI
                     YGTDASPHNGWDTFILIALLTLCFLSFIANTHTFYEACYKRPLLVFICRTIRLIKSKK
                     MTPTKTKCTVAVALAKRKSIELVEVLISFVLLQITLAVVITSAI"
     gene            2462..>8953
                     /locus_tag="PKZ52_05605"
     CDS             2462..>8953
                     /locus_tag="PKZ52_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG59281.1"
                     /translation="MGTIFGGLASLVQAGPENGHIVNGQGAITQVEKETRIQQASERL
                     SIDWKSFDINSDERVQFIQPNSSSIAFNRILSNKGSLIQGRIDANGQVVLINPNGLIF
                     TEGASVNAGGLIASALQMNDQDYLNGKFTLNALEGSEGRVINSGLLNAATGGTISLLG
                     QSVENKGLISAHLGSVNLAAGKEAVLTFDPNGLVGVRVTQAVLQKALGVDAAIINSGE
                     VRAEGGKVLITASTSKDIFSKAVNVGELSSARSAVVNDDGSFTLGAGADIKNTGVINL
                     GAEVKAGEVVLIGENITNQGNINADSLTDYAGSIEITAKNINETKDNGSVTAIAHKSG
                     KGGDIKLLGDKIGLLDSANIDASGANGGGQILIGGDKTGINHLIKNAQFIYLGEDSRV
                     SADAFQSGDGGKLIAFADDTARIYGGLFARGGINVGSGGFIETSGIHGLELGSTPDIT
                     AGNGSAGTWLIDPFDLIISDGVSSSVTVSGKTYTSGGSRATVGWSAINTALGAEGATV
                     IIQSGGSSATQVGDVTFNSAAQNYLFNFDGVGNIKSTLKINAHGDIKFSDKINGLSAS
                     TTNNNRLNLEFTAGGDIDLNKVAISTNGGYFTATAKGTQKLFSNPDVLAAGDFKAADA
                     TISTYAAFGGGDILINAKGKADLGAMDFSYDYRNGNEFKLTQVGSINVTAGSTIILNK
                     EFDFNDSGGYAADGTANAISDRTALTFTAGDSITLNGSIIDKEYGDGRDALGINLTAG
                     TGSVTINQPIYTSGGSVVISGNGINQNALINTDRANERTTVEQANGKNGNATRSEGGD
                     VTLSSDTALALNAITTDSFCADGKCTGNLVVQSRTTGKNIDITQTDNTFLKIHGTTDF
                     IINGGTLNLTNGLNIFGTQGGSVEEQAGNAVSKTITLGKTGNTTVNGFSLLLGAADIN
                     GTARLTATSPTASVNSITQAANSKLTITGNTELTADTITLDHDGNDFATITSIDAATS
                     AHISDINSVDFNTLKTANLTLNTQADVHFNGPIATQDKAVAITQKGTGAVALADGLTQ
                     TGTGVVTFKGTALSGGLQYLGEQNTVWNLAEKALVWSGKTVGFAGFSRLIGGDGGDTV
                     SLSEAVTAIDLANQNSTGFALSNMEVIKATAANTSLKGFKANSQWTIIDDKKGTIVQA
                     GGRPTAFEGFNELISSDDSANSDTFIFNSAGAVGKITGQNADVVNLFTGADGAGVDYD
                     WAVTANTLNGFTTASTQVKRSGQSTDVISALTGVGTINSSNGKDTVSASATYAGTIDL
                     KAGVNALTTDSASLLFSSSPLKMNGFVGVNGLNAKTQAATLTATTDNASATLAWTVNP
                     AEDALKGPLSGSITYAQTGQADQTLNFNRFALLSGGANNDTFTLNAGTFTGGQINAGT
                     PGSTATQDRLINAVDGTLWDLTAASLRGVTFSGFNYWQGSGTDTVNGMNATNNWVMNA
                     GHFTLNTATQLSGFNAVNGGSGKDIFTITANTSGITLRGNAGADEFILADSRIQTGTL
                     DGGAGVGVDSENDTLKINSGTNSGTNSSTNAWVVTAPKTGTVNGNHFSGMEILAGGTG
                     SDTLDNTIANPTFLLTGVGAGILQSKTPEPSPIAATVTGMDTIKNTDELKGLDNGTLT
                     WSITGTDKGAIKNAAQQTLINFERVAQLTGGSANNVFELTPTGAPLTLSIVGGQGINS
                     LKAPNTINTWNMSDATSGVAAAGSATGLRFKGIQKIYGGTEKDTLDYSAVPVTSPLTI
                     NAADLVSIEAVNGNGASVALQAGDDSNIWTLNDAGGGLLNNTLAFSQVVGVRGGAGKD
                     RFIITQTSAFNGTLNGGANAANTQDELSVDALASEFLITAQGEGALSFKNLINTPSQL
                     KFAGIEVLNGGEAVDTFRFSSALTDAANLNMLLDGKGNQDSIELPKIQLEISLAAGVE
                     RTGNQGLVLANFEAIAADESTNSSLRAVNGTAQWAIYGRNAGALTLTNDVNVSTVTHF
                     SGFLNLIGGSGADTFNFNVPDAAGLVQGRVTGTLTGGAGDRLIARDTANEWTLTAASE
                     GKNSLKTQAGELYVADFTGVSHLQGGSEQDRFTISGNQNAISIYGGAGNDMFTLSDSG
                     FIKNISGEEGINTLEGRTSGSMAWNFTSATNGQLKEGALLLADFNDIQNFKGRSASDT
                     VNYFSSPTSGSVKTWEYDSNQFKVVEDKITLV"
BASE COUNT         2441 a         1791 c         2281 g         2440 t
ORIGIN      
        1 aatggaaatc gcataatgaa ggtgttctcc ctgaagatat gctgcaatat gggatcgata
       61 tttcaccgct gtacagtgtt agggggaagg agtacgttcc ttattttggg tatggtcgaa
      121 cagggaaagg taatattttc attgagactg cgacggggca cggtgcggta atttattatt
      181 attactcatt tccaattgct attgacatgt atttagagtt tgaatttgtg ggggcgccgg
      241 atattaatgg gtctccttat ttgtttcaag agcaggttag gggtaggata aaatcaatta
      301 cagatagctt gcagctatcg ttgcgagaca atcatagttg catgggtggg gttttgggga
      361 agtgggtaat tgctgattcg gtaaagttgc tccagcctga tcctttaacg ctacctaagc
      421 atattcctgt cgatgagatt aaataaaata tgtataggtg aaagggctgg gagtaatgga
      481 gtgcgtaaaa gcggcggtgt aaattaattt tttattggcg gtggctttaa ttgtgacaat
      541 ctttacttca tttatggcac atgcgaagga cccaacaatg tatccgcgag ttattcagct
      601 gcctgatgcc gagttgcggt tcagcatgat ggaggacttt tctaatgata tgccagccga
      661 gcctctaatt gagaagatcg aatcagctaa tctaagtgaa ctaaaaaata taactgaccg
      721 ttttgttata gggcgccgct ggtgggatct caagccacca ggctggttta aaaagtcctg
      781 gggtagtatt caaatgactt tgatgctagg tggtgccatt aataaacagg gtgcaaaatt
      841 agatttttgt acttttagtc aggcagattt tatggttttt taccatgaag ctttggtaga
      901 aaaatggcgc aaacataatg aaagcattac gcctgaaaat cagatccaat ttggcgtcta
      961 tgtgatgtct ctttttggtg ccatagggca ggaatttatc cctcattctg gattcaaaca
     1021 aacaggccat aatgctttgc tgttggagtc tgtggccgct caggatgcaa atatatactc
     1081 ttattattct atccctgtcg gcagagatac ttttttggaa tttgagttta ttggtgcgcc
     1141 agacttaaat ggttcgcctt atttatttca agcgcaagca gatgagagga ttaattctat
     1201 acggcaaagt gttgcattaa aattggatca aaaccgaagt tgcgtggctg gcgtgcttga
     1261 taaatgggtg gttgctgatt cagttcagtt gctaaagcca gacccatcgc tacttcccaa
     1321 accagcaccc tctggtacgc ttgaaaagtt ctttccggaa aagaaaggcc catagatttg
     1381 gtacgtcaat atgacttaat cctgaggggc agttgtgtta ttttcgattt gcttgcattt
     1441 gattttataa gatagattct acgtaggctc gtaaaccact tcgaaataag cattggctat
     1501 tttgagtttt attcgggtgg ctgtctaatt ctgcatggcc acgtactttt gaaactattt
     1561 tcatgatttc aaaaatagag acgtccattt taatgcgata tcgtttttct tatctttttc
     1621 tggtttttgg ctgtccgtta taattgctat tttttgacat tatgttcgtg tgcgggagga
     1681 tggtttgatc tgtacagggt attggcttgc tatatggcgc ttgtaatcac tactgcaagc
     1741 gttatctgca agagtacaaa tgagatcaag acttctacca attctatgct tttgcgtttt
     1801 gctagggcta ctgccacagt acattttgtt tttgttggcg tcattttttt gctcttgatc
     1861 aagcgtatcg ttcggcagat aaaaacaaga agtggtcgct tataacaggc ttcataaaat
     1921 gtatgggtgt tggcgataaa ggacaggaaa caaagtgtta gaagcgcaat aagtatgaat
     1981 gtatcccatc cattgtgggg gcttgcgtcg gttccgtaaa ttttatacgc cgccaataag
     2041 gcaaaaacaa gtaatgctga ttgcggtgtt agatttctta ggtattcaag gtaggctttt
     2101 ttgccatggt ttaacacggc aatgcgtgat aagagtgtcc gtttgcgcat agggtagtta
     2161 ctctctggct atataaatgg aatttgattt ttggtgtatg aattgcgggg gcagcgctca
     2221 gcgcgatgat attctttagt gttgacctgg tcaatgtcaa tattttttcg gtcatggtct
     2281 ttttttgtaa ctattattct ggcttttgtc agcatcgatc ggtgcggata tgtaaaattt
     2341 attttcagga ttgatcttta ggatggccta tttatcggcc tgtcattttg catagtgaat
     2401 gtggattttt atgttgcgac atgttttttc ttttcaatca agacatattt taatctgtgc
     2461 tatgggtact atttttggtg gtctcgctag tttggttcag gctggccctg agaatggtca
     2521 tattgtaaat gggcaaggtg ccataacaca ggttgaaaaa gaaacccgta tccaacaagc
     2581 cagcgaacgt ttaagcattg attggaaaag ctttgatatt aatagcgatg agcgagtgca
     2641 atttattcag cccaatagct cttctattgc ctttaaccgt attttaagca acaaaggctc
     2701 attgattcag gggcgcatag atgccaatgg gcaggtggta ttaattaatc ccaatgggct
     2761 tatttttacg gaaggggcca gtgtaaacgc tgggggttta attgcatccg ctttacaaat
     2821 gaatgatcaa gattacctta atggtaaatt cacgttaaat gccctagaag gcagtgaggg
     2881 ccgggttatt aattccggcc tgttaaatgc cgccacaggc ggcactatca gtttgctcgg
     2941 ccaaagcgtg gagaataaag ggttgataag tgcccatttg ggtagtgtta atttagcggc
     3001 gggtaaagaa gccgttttaa cctttgaccc caatggtttg gtgggtgtgc gggttacgca
     3061 ggcggtattg caaaaagcct taggtgtgga tgcagccatt attaatagcg gggaggttag
     3121 agctgaaggc ggtaaagtat taattacggc cagcaccagc aaagatattt ttagcaaagc
     3181 tgtgaatgtg ggggagttat cgtctgctag aagtgccgtg gtgaacgatg atggcagttt
     3241 caccttgggc gctggagcgg atataaaaaa taccggagta ataaatttag gtgcagaagt
     3301 taaagccggt gaagtcgtac taattgggga aaacattact aaccaaggca atatcaacgc
     3361 cgatagccta accgactatg cgggcagtat tgaaattact gcaaaaaata ttaacgaaac
     3421 aaaagataac ggctccgtaa ccgccattgc gcataaaagt ggcaagggcg gggatattaa
     3481 attactcggc gataaaatcg gtttgttgga tagcgctaac atagatgctt caggtgcaaa
     3541 tggcggtgga caaattctaa tagggggcga taaaacaggc attaaccacc tcatcaaaaa
     3601 tgcgcaattt atatatctgg gcgaggattc aagggttagc gccgatgcat ttcaaagcgg
     3661 cgacggcggc aaactaatcg catttgcaga tgatacggca cggatctacg gaggtttgtt
     3721 tgcgaggggt ggtataaatg ttggtagcgg tggattcatc gaaacttccg gaattcatgg
     3781 tttggaattg ggcagcacgc cagatataac ggctggcaat ggttctgcag gaacatggtt
     3841 gatcgaccca tttgatttaa ttattagtga tggcgtttcc tcttctgtaa ctgtcagtgg
     3901 taaaacttat acttcgggcg gctcccgtgc cactgttggt tggagtgcta ttaatactgc
     3961 gttaggggca gaaggagcta cagttataat tcaatcaggg ggatcaagtg caactcaagt
     4021 tggcgatgta acatttaatt ctgctgcgca aaattattta tttaattttg atggtgtagg
     4081 gaatattaaa tccacgctaa aaataaatgc acatggcgat ataaagttct cagataaaat
     4141 taacggctta tctgcctcta ccactaataa caaccgcctc aatttagaat tcaccgcagg
     4201 cggtgatata gatttaaaca aagtggcgat ttcaaccaat ggcggctatt tcaccgcaac
     4261 cgccaagggc acacagaagt tattcagcaa cccagatgta ttggccgctg gtgattttaa
     4321 agccgccgac gcgaccataa gcacctacgc agcattcggt ggcggagata ttcttatcaa
     4381 cgctaagggc aaagcggatt taggagctat ggattttagc tatgattacc gcaacggtaa
     4441 tgaatttaaa ttgacccagg taggttcgat taatgtaact gcaggcagca ctattattct
     4501 caataaggaa tttgatttta atgatagcgg tggttatgcg gcggatggca ccgccaatgc
     4561 tattagtgat agaacggcgc ttacattcac tgctggtgat tctattacgt taaacggttc
     4621 cataattgat aaagagtatg gcgacgggcg cgatgcatta ggtattaatt taacggcagg
     4681 tacaggcagc gtcacgatca accaacctat ttatacgtcc ggtggctcag ttgtaatttc
     4741 aggtaacggt attaaccaaa acgcgctgat taataccgac cgcgccaatg agcgcactac
     4801 ggttgagcaa gccaatggca aaaatggtaa cgctacccga tcagagggtg gagatgttac
     4861 gttaagcagt gacaccgcct tggctttaaa tgccattacc accgattcgt tttgtgccga
     4921 cggaaagtgc acgggcaatc ttgtggtgca aagccgtaca acaggtaaaa atatagatat
     4981 tacccaaacg gataacactt ttttaaaaat ccatggcact acggatttta ttattaatgg
     5041 cggcacactt aatttaacca atggattgaa tatttttggc acccaaggcg gcagtgttga
     5101 agagcaagcg gggaatgcgg tttctaaaac cattacgctg ggcaaaaccg gcaataccac
     5161 agtaaacgga tttagtttat tgctgggtgc cgcagacatt aatggcaccg cacgtttaac
     5221 cgccaccagc ccaactgcct cggttaattc tattacccaa gccgctaatt caaaattaac
     5281 gattacaggc aacacagaat taacagccga caccattacc ctcgatcatg acggcaacga
     5341 ctttgccacc attacctcca tagatgcagc aacctctgcg cacatatcgg atattaatag
     5401 cgtggatttt aacacgctta aaaccgccaa tttaacgctc aatactcaag cggatgtgca
     5461 ttttaatggt cctattgcca cgcaagacaa agccgtggcg attactcaga aaggtacagg
     5521 tgctgttgcc ttggccgatg gcctgacaca aaccggtaca ggagtggtta cgtttaaggg
     5581 cacagcgctg agtggagggt tgcagtattt aggtgagcaa aatacggtat ggaatttagc
     5641 ggaaaaagca ctggtgtggt cgggtaaaac tgttggcttt gcgggtttta gccgtcttat
     5701 aggcggtgat gggggggata ccgtaagcct gtcggaggca gttacagcga ttgatttagc
     5761 taatcaaaat tccactggtt ttgctttgtc gaatatggaa gtgattaaag ccactgcggc
     5821 taatacgagc ctaaaaggct ttaaagctaa ttcacaatgg acaattatcg acgataaaaa
     5881 aggcacgatt gtacaagcgg gcggcagacc tacagcgttt gaaggtttta atgaactaat
     5941 tagctcggac gatagcgcga atagcgacac ctttattttt aatagcgctg gtgctgttgg
     6001 caaaattact ggccaaaacg ctgatgtggt taatctgttt acgggcgcag atggcgctgg
     6061 ggtcgattat gactgggccg ttacggctaa taccctgaat ggttttacca cggcctcgac
     6121 ccaggtaaaa cgcagcggcc aaagtacgga tgttatttca gcgcttaccg gcgtaggcac
     6181 tatcaacagt agtaatggca aggataccgt ttctgcgtca gcgacctatg caggcactat
     6241 tgatttaaaa gcgggggtta atgcgttaac caccgattcc gcgagccttt tattttccag
     6301 cagcccatta aaaatgaatg ggtttgtggg cgttaatggc cttaacgcca aaacccaagc
     6361 cgccacctta acggccacca cagacaatgc cagcgccacc ttagcctgga cggttaaccc
     6421 ggcggaggat gcgcttaaag gcccgttaag tggatccatt acctatgctc aaaccgggca
     6481 agcggatcaa accctgaact ttaatcgatt tgcactttta tccggtggcg ctaataacga
     6541 cacattcact ttaaatgcag gcacatttac cggtgggcag attaatgccg gtacacctgg
     6601 cagtaccgcc acgcaagatc gattaattaa tgcggtagac ggcaccctat gggatttaac
     6661 cgccgcgtca ctgcgcggcg tgacgtttag cggatttaat tattggcaag gcagtggcac
     6721 ggataccgta aatgggatga atgccactaa taactgggtt atgaatgccg gtcacttcac
     6781 tttaaatacg gctacccagt taagtggttt taatgcggtt aacggtggca gtggtaagga
     6841 tatttttacg attactgcca atacatcggg tataaccctg cgcggcaatg cgggggcgga
     6901 tgaatttatt ctggccgata gccgtattca aactggaacc ctagacggcg gtgcaggcgt
     6961 gggcgtagat agtgaaaacg acacgcttaa aataaattca ggcactaatt caggcactaa
     7021 ttcaagcacc aatgcctggg tcgtaacggc gcccaaaacc ggcaccgtga atggtaatca
     7081 ttttagcggt atggaaattt tagcgggcgg cacgggcagt gatacgctgg ataataccat
     7141 cgctaacccg acatttttat tgaccggagt aggcgcggga atcctgcagt caaaaacccc
     7201 agagccatca cccattgccg ccacggtgac cggcatggat acgattaaaa ataccgatga
     7261 attaaaaggc ttggataacg gcaccctcac ctggagtatt acgggtacgg ataaaggcgc
     7321 cattaaaaac gccgcacaac aaaccttaat taattttgag cgcgtggcgc aattaaccgg
     7381 cgggagtgcg aataatgttt ttgaactcac cccaaccggt gcgccgctaa ccctgagcat
     7441 tgtcggtggc caaggcatta attctttaaa agcgcccaat acgattaata cctggaacat
     7501 gtccgatgct accagtggcg ttgctgctgc tgggagcgca acaggtctta gatttaaggg
     7561 tatccaaaag atttacggtg gtacggaaaa agacacctta gattattctg ccgtgccggt
     7621 tacctcgcct ttgacgatta acgcggcgga tttagtgtct attgaagcgg tgaacggcaa
     7681 tggtgccagt gttgccttac aggcggggga tgactccaat atctggacat tgaacgacgc
     7741 cggtggcggt ctattaaata acaccttagc ctttagccag gtagtgggtg tgcgcggagg
     7801 tgcgggtaaa gaccgtttta tcatcacgca aaccagcgcg tttaatggca cgttgaacgg
     7861 cggtgccaac gctgcgaaca cgcaggatga actgagcgta gatgcgttgg cgagtgaatt
     7921 tctgatcacg gcgcaaggcg aaggcgcgct gagctttaaa aacctgatca atacgccttc
     7981 acagctgaag tttgcgggca ttgaggtgtt gaacggaggc gaggccgtgg acacgtttcg
     8041 attttcctcc gcactcaccg atgccgccaa tttaaatatg ctgctggatg gcaaaggcaa
     8101 tcaggattcg attgaattac ccaaaatcca attagaaatt tccctcgcgg cgggtgttga
     8161 gcgcacgggt aatcaaggct tggtgttagc taattttgaa gcgattgcgg ctgacgaaag
     8221 taccaacagc agcctgcgtg cggtcaacgg tacggcccag tgggctattt atggccgcaa
     8281 cgcaggggcg ttaacgctca ccaacgatgt taacgtcagt acggttactc acttcagcgg
     8341 gtttttaaat ctaatcggcg gcagtggtgc agataccttt aactttaatg tgccggatgc
     8401 tgcaggttta gtgcaaggcc gcgttaccgg taccctcaca ggcggcgcgg gcgatcgttt
     8461 aatcgcgcgg gatacggcaa acgaatggac attaaccgct gccagcgagg gtaaaaattc
     8521 acttaaaacc caagccgggg aattatacgt tgccgatttt acaggtgtca gtcacctgca
     8581 agggggcagt gagcaggatc gttttactat tagtggtaat caaaacgcta tcagtattta
     8641 cggtggcgcc ggtaacgata tgtttacgct cagcgatagc ggatttatta aaaacattag
     8701 tggcgaagag gggattaaca cactggaggg acgcaccagc ggcagcatgg cgtggaattt
     8761 tacctcggcc acgaacggtc aattaaaaga gggtgccttg ctgttggccg attttaacga
     8821 cattcagaat tttaaaggcc gctccgcgag tgatacggtt aattatttta gttcacccac
     8881 gagtgggagt gtcaaaacct gggagtatga cagcaaccaa tttaaggtcg tcgaggataa
     8941 aataaccctt gtc
//
LOCUS       DAOQGS010000104         8951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3675_length_8951_cov_4.527765, whole genome shotgun sequence.
ACCESSION   DAOQGS010000104 DAOQGS010000000
VERSION     DAOQGS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3675_length_8951_cov_4.527765"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..2392
                     /locus_tag="PKZ52_05610"
     CDS             236..2392
                     /locus_tag="PKZ52_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG59282.1"
                     /translation="MTRFSLGAVWRRASLVCMASALALGLAGCGGDPAASSSSSSSSS
                     SSSSSSAGSSSSSSSGGTWSQTGNPALGEIAYGQQCLGCHGKQGSSDINPKALKVAAA
                     NTNLAKYIHDSMPLGKPANCVNECAANTAAYIRSWASTGSSSSSSSSSGSSGVALDGK
                     SLFASKCAKCHGSNGEGIVDVAPALNKETFNESYLLTKIPTMPGACSGDCAAAVAKYI
                     RATFTPAQSVCSGSESPLPKQLRLLTRTEYRNTVNDLFGISSTVAESLPITDPVGGYD
                     NNAKASGVSESHMGKYWDAAGILAETVNISSINTCNQKTTSCGGTIVDTFGPKVFRRP
                     LTSAERSDYIDLFMYASDAAQGTRYLIRAMLSSPNFLYRSEVGNNGTLTPYETASLLS
                     YTFWGSMPDATLTSAAANNQLSTPAQLRSQATRLLADAKARRHMAYFARQWLGAQQVA
                     EINKDLNIFPQLTSSLRSALDTEFDMFIQDVFFNSSSKFADIYLANYTYANSTLAQFY
                     GLSGAGSSFAKINSGGLRGGVLDMGAVLASHAQFNQTSPIKRGKFVRERLLCQELPPP
                     PPGLNAKPPALDPSKPTRDRFAAHTSDPSCQNCHQFIDPIGFSFERYDGIGAYRTSEN
                     GLNVDDSGSLRGLINRTDTDVTDYNGTQELAQILATSTSGSACLAKQFEVYVSGNAHP
                     DQCTVQNVVSRWSNKAYVLPELWLEAVSSESFLKRN"
     gene            2406..3680
                     /locus_tag="PKZ52_05615"
     CDS             2406..3680
                     /locus_tag="PKZ52_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG59283.1"
                     /translation="MKRRSLIQGIVAAPLWTALPLSHLITRTAMAADRPIRTLFIYHP
                     DGCTPELWHPAASSSSPSLKAQSAPLEKVKQYCTFMDGFYLVGKGDTHEGGAVKVLTA
                     NDASSSQGSTSSSIEVALGQLSPTLFKSYQIGCFGGYWADKSICFNGSSRLPIEDNPQ
                     NAFKTLFSSGSSSSGGGNTNASQLRILDNVSKDLARLRSQLGSIEKERLDMHAESFKS
                     LQDRLTAQGNQSGGTAACVSSGINLQGLGANEARSVEKLKTISEVQQDIIIQALSCDL
                     VRNVVFSYSHPVSPIVVPGSSMGDHDASHASPSQFTPSKTWWIGQIANMLDKMSKIPD
                     GTSGSLLDNTIVLLVSELGDSALHDHWRVPFVLAGGKNTGLKGNRALDFRSANGGKGA
                     NHGDLLTAIAQKAGYTSISKFGLASGPMNGIW"
     gene            3764..4732
                     /locus_tag="PKZ52_05620"
     CDS             3764..4732
                     /locus_tag="PKZ52_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNG59284.1"
                     /translation="MAERFMFDTYFPLYHGAPLGNADFRCHWDDFVVDEELPPMDLGA
                     GEHVYLQIRKRGINTEWLARQLAERLKIAPNDVGFCGLKDRHATTSQWFSLYLPKVKE
                     LDGRALLPEIEGELELLQQTRGSKKLRRGMHKANHFVIHLRHYSGCQDAFAARLELIR
                     QQGVPNYFGEQRFGIEGQNLLAAKTWLEEKQRIKRSLEGIYLSSLRSYIFNAILRARL
                     ASAPLEELQTLSGALWGRGRLPAEYAEFETQALHADLAPLLNALEHKGLNQERRPLWL
                     QVADLTWQFPGPDQLQLRFSLAPGQYTTSLLRESVHLRDMSVKIHS"
     gene            4899..5678
                     /locus_tag="PKZ52_05625"
     CDS             4899..5678
                     /locus_tag="PKZ52_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNG59285.1"
                     /translation="MKLIPNMAATQSHIGQLVKILIACLILSSCSTHSARSTKITTTA
                     TPKPAPAGLYRVQAGDTLFTLAWKNSTTVENLQKINGMGQSSALRAGQLIRLSGEPES
                     PPSRAQNIVRSEPAGPCCIKKQPDKPEPIKKYELSKWTSGWIWPIKGPVVSGYSAKGG
                     LNKGIDIKSKLGESVVAASDGEVVFAGNELKGYPNLIIVKHGARYLSAYSNNHALLVK
                     EGDKVRKGQPIAHMGKVPEATRLHFEIRADGKPIDPQQLLP"
     gene            5843..6853
                     /gene="rpoS"
                     /locus_tag="PKZ52_05630"
     CDS             5843..6853
                     /gene="rpoS"
                     /locus_tag="PKZ52_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNG59286.1"
                     /translation="MASPAVDFDTLKTDDLHLMNEIDDIITIDDIALIDSDDFDLVPR
                     GREGVIEGEDPNPKNLDATQLYLNEIGFSPLLSAEEEVFFARKALKGDNAARKRMIES
                     NLRLVVKISRRYVNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIE
                     RAIMNQTRTIRLPIHVVKELNVYLRAARELSQKLDHEPSPEEIAALLEKPVEDVERML
                     ALNERVTSMDAPIGPSSDKTLVDTVPDVHVSDPVELLENSDLNLSLDHWLDELTEKQR
                     EVLSRRFGLRGHESSTLEEVGREIGLTRERVRQIQVEALRRLRDILEKQGLSAESLFG
                     IM"
     gene            7373..>8951
                     /locus_tag="PKZ52_05635"
     CDS             7373..>8951
                     /locus_tag="PKZ52_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNG59287.1"
                     /translation="MTFAVSFRPSPRFAFSRRLLSSAVLSGLGLMASSAELLAKAPPH
                     ELPAANKTTPVLLPAQWNQVVNPVPANPALEAKIAEILGKLTLEQKVGQMIQPHIGAV
                     TPEEVKTYHIGSILNGGGQNPNDNPNSTAQDWAQLAERFYQASMSSDNGRTPIPVIWG
                     IDAVHGHNNLVGATLFPHNIALGATRNPALVKSLGALTAAEVYATGQDWDFAPTLAVA
                     QDDRWGRTYESFSEEPSLVAQMGGKFVEGLQGTAGTKDFLAAKKVLATAKHFIGDGGT
                     QYGDDQGFTEGEEQALLRTHLPGYISALNAGVQTVMASYSSWNGVHSHANARLMNQLL
                     KGQLGFDGFIVSDWQAVSHVPGCSLDNCPEAVNAGVDLFMIPNAPDWKTFHANVVAQV
                     KAGVISQARIDDAVRRILRVKLRQGLFTAPSPLQRAAASSGVIGSAEHRALARQAVRE
                     SLVLLKNTQGVLPIKPKSKVLVAGIGGNNLSRQSGGWSVTWQGIDVPNDKFPGATSIF
                     KGIADAVKAVGGSAEYSE"
BASE COUNT         2179 a         2597 c         2274 g         1901 t
ORIGIN      
        1 ggtcaacctc acaagcaggt tattgtcccg aagaacacaa gcatcacgct taacttgcgg
       61 ggcaattcac agatattcat ttgcagctgg ccgatgtgaa atctcaccgg ctaaagccac
      121 cgaaaccacc taaaatctgc cgttaggtta ttagctgtgg ctgatagcaa accgaattaa
      181 ctaagaaccg ctttgggcta gaagctcaac aaggggcaaa tccgtggata tatctttgac
      241 tcgtttcagc ctgggtgcgg tgtggcgccg cgcaagcctg gtgtgcatgg caagtgctct
      301 tgctctgggc ttggccggtt gtggtggcga tcccgctgca tccagcagct ctagctcttc
      361 aagttccagt tcctcaagct cgtccgcagg cagcagttca agtagctcct ccggcggcac
      421 ctggtctcag acgggcaacc ccgccttggg cgaaatagcc tatggccagc agtgtttagg
      481 ctgccacggc aaacagggca gttctgacat caaccccaaa gcattaaaag ttgccgctgc
      541 caataccaat ttggctaaat acatccacga cagcatgccg ctgggcaaac ccgccaactg
      601 cgtcaacgaa tgcgccgcca ataccgcagc ctacattcgc tcctgggcca gcaccggcag
      661 cagctcaagc tccagcagta gctcaggctc ctcgggcgta gccctggacg gcaaatccct
      721 attcgccagc aagtgtgcca agtgccacgg cagcaatggc gagggcatag tggatgtagc
      781 accagcgctg aataaagaaa ccttcaacga aagttacctg ctcactaaaa ttcccaccat
      841 gcccggcgcc tgctcggggg attgtgcagc ggcggttgcc aagtacattc gcgccacctt
      901 taccccggca cagtcagtgt gcagcggttc agagtcgccc ctgcccaagc aactgcgcct
      961 gttaactcgc accgaatacc gcaacacggt taacgacctg ttcggcatta gttcaaccgt
     1021 tgccgaatcg ctccccatca cagatcccgt gggcggctat gacaacaacg ctaaggcctc
     1081 cggggtgtcc gaatcccata tgggtaaata ctgggacgct gcgggcattt tggccgagac
     1141 ggtgaacatc agcagcatca acacctgtaa ccaaaaaacc accagctgcg gcggcactat
     1201 tgtggatact ttcggcccga aagtattccg ccgccctcta acctccgccg agcgcagcga
     1261 ttacattgac ctgtttatgt acgccagcga cgcagcccaa ggcacccgct atttgatccg
     1321 cgccatgttg tcctcgccca acttcctcta ccgctctgaa gtgggcaaca acggcaccct
     1381 caccccctac gagacggcaa gcctgctctc ctacaccttc tggggcagca tgcccgatgc
     1441 caccctcacc agcgctgcgg ccaacaacca gttgtctacc cccgcacaat tgcgcagcca
     1501 ggccacgcgc ctgttggccg atgccaaagc ccgccgccat atggcctatt ttgcccgcca
     1561 gtggttgggc gcccagcagg tggctgagat caacaaagac ttgaacatct tcccgcagct
     1621 gaccagctcg ctgcgctccg ctttggatac cgagtttgat atgttcatcc aggacgtatt
     1681 ttttaattcc agcagcaagt ttgccgatat ttacctcgcc aattacacct atgccaactc
     1741 caccctggcg cagttttatg gcctaagcgg cgcaggcagc agttttgcca aaatcaactc
     1801 cggcggcctg cgcggcggcg tgttggatat gggggccgtg ttggcaagcc acgcccagtt
     1861 caaccaaacc tcccccatta aacgcggtaa gtttgtgcgc gagcgcctgc tgtgccagga
     1921 gttgccgcca ccgccgccgg gcctaaacgc caaaccaccg gccctggacc ccagcaaacc
     1981 cacccgggat cgttttgctg cccacacgtc ggatccatcc tgccagaact gccaccagtt
     2041 catcgacccg attggcttct cgtttgagcg ctacgacggt atcggcgcct accgcaccag
     2101 cgaaaacggc ttgaacgtgg acgactccgg cagcctgcgc ggcctaatca accgcaccga
     2161 caccgacgtc accgattaca acggcaccca agagttggcg caaattctcg ccacctcaac
     2221 ctcaggctcc gcctgtttgg ccaaacagtt tgaagtttac gtatccggca atgcccaccc
     2281 ggatcagtgc actgtgcaaa acgttgtttc gcgttggagt aacaaggctt atgtactgcc
     2341 tgaactctgg ctggaagctg tttcgtccga atccttcctg aaacgcaact aagaggagcc
     2401 ctcccatgaa aagacgctca ttaatacaag gcatagtagc ggcacctctg tggacggctc
     2461 ttcccctgtc ccatttgatt acccgcaccg ccatggcggc ggaccgcccc attcgcaccc
     2521 tgtttattta ccatccggac ggctgtaccc cggagctgtg gcacccggcg gcttcgtctt
     2581 ccagcccatc attgaaggca caatcggcgc cgcttgaaaa agtgaagcaa tactgcacct
     2641 ttatggatgg cttttattta gtgggcaaag gcgataccca tgagggcggg gcggtaaaag
     2701 tgctcaccgc caacgacgcc agcagcagcc agggcagcac ttcatcctcc attgaggtgg
     2761 cactggggca gctctccccc accctgttta agtcctatca gatcggttgt ttcggcggct
     2821 attgggcgga taaatccatt tgttttaacg gctcatcgcg cctgcccatt gaggacaatc
     2881 cgcagaatgc cttcaaaacc ctgttcagtt ctggctccag cagcagcggt ggcggcaaca
     2941 ccaacgccag ccagctgcgc attttggata acgtcagcaa agaccttgcg cggctgcgca
     3001 gccagctggg cagtatcgaa aaagagcgcc tggacatgca cgccgaatcc ttcaaaagcc
     3061 tgcaagatcg cctaaccgcc cagggcaatc agtccggcgg cacggcagcc tgcgtaagtt
     3121 ccggcattaa cttgcagggc ttgggcgcca acgaagcgcg cagcgtggag aaactcaaaa
     3181 ctatctcaga agtgcagcag gacattatca tccaagccct ctcctgcgat ttggtgcgca
     3241 acgtggtgtt ctcctactca caccccgtgt cccctattgt ggtacccggc agctccatgg
     3301 gcgatcacga cgcctcccac gcctcgccct cccagttcac ccccagcaaa acctggtgga
     3361 tcggccagat cgccaacatg ctggacaaga tgtcgaaaat ccccgacggc accagcggca
     3421 gcttgctgga taacaccata gtgctgttgg tttcagaact gggcgactcc gccctgcacg
     3481 accactggcg agtgcccttt gtactggctg gcggcaaaaa cactggcctt aagggcaacc
     3541 gagcgctgga ttttcgcagt gccaacggcg gtaagggcgc taaccacggc gacctgttaa
     3601 ccgccattgc acaaaaagcc ggttacacct ctataagcaa gttcggcctg gctagcgggc
     3661 ccatgaacgg catctggtaa atacagggcc aggttgctgg cgataattcc agccataaat
     3721 cgttacaatg cgcggccgca gtccttttga ctgcggcttt tttatggccg agcgatttat
     3781 gtttgatacc tactttcccc tctaccacgg cgcccccctg ggcaacgccg attttcgctg
     3841 ccattgggat gattttgtgg tggacgaaga actgcccccc atggatttag gcgcggggga
     3901 acacgtctat ctgcagattc gcaaacgcgg catcaatacc gagtggctgg cccggcagct
     3961 ggcggagcgg ctgaagattg cgcccaacga tgtggggttt tgcggcttaa aagatcgcca
     4021 cgctaccacc agccagtggt ttagcctgta tctacccaag gtcaaagagc tcgatggccg
     4081 cgcactgtta cccgagattg aaggtgaact ggaattgctg cagcaaaccc gcggcagtaa
     4141 aaagctgcgc cggggcatgc acaaggccaa tcattttgtc attcacctgc gccattacag
     4201 cggctgccaa gacgcctttg cggcgcggct agaattgatt cgacaacagg gcgtgccgaa
     4261 ctattttggt gaacagcgct tcgggattga gggccaaaac cttctggccg ccaaaacctg
     4321 gctggaagaa aagcagcgca tcaaacgcag cttggagggg atttatttaa gttccctgcg
     4381 cagttatatc ttcaacgcca tcctgcgtgc gcgccttgca agcgcgccac ttgaagaact
     4441 gcaaaccctc agtggtgccc tctgggggcg cggccgcttg cccgctgagt atgccgaatt
     4501 tgaaacccaa gctctgcatg cggaccttgc ccctctactt aacgccctag agcacaaagg
     4561 gcttaatcaa gagcgcaggc ctctgtggct gcaggttgcc gatttaactt ggcaatttcc
     4621 cggcccggat cagctgcagt tgaggtttag tttagcgccg gggcagtaca ccaccagcct
     4681 attgcgggag tctgtgcatt tgcgggatat gagtgtgaag attcacagct gaagcctccg
     4741 ttaaatatta ctagtaaggc attaatttaa ctaatgctta agtttatata cccgaactga
     4801 attattattt tttgtaattt ttagatttat ttgcaatata agatacggaa aatatcgatt
     4861 ttataaatat aaaatagagt aaaaagtgac gaatgtgcat gaaacttatt ccgaatatgg
     4921 cggccactca gagccacatc gggcaattgg ttaaaatttt aatcgcgtgc ctgatcttat
     4981 ccagttgtag cacccactca gcgcgcagca ctaaaataac cactaccgcc acccccaaac
     5041 ccgcccccgc tggcctctac cgagtacaag cgggagacac attgtttacc ctggcttgga
     5101 aaaacagcac taccgttgaa aatttgcaaa aaattaacgg catggggcaa agctccgcct
     5161 tgcgtgcagg ccaattgatt cgcttaagtg gcgagccgga aagcccccca agcagggcgc
     5221 aaaatatagt gcgcagcgaa cctgccggcc cctgctgcat aaaaaaacaa ccagacaaac
     5281 cagaaccaat aaaaaagtat gagcttagca aatggacttc tggttggatt tggccaatta
     5341 aggggccggt tgtttccgga tactcagcca aaggtgggtt aaacaagggt attgatatta
     5401 agtccaaatt aggagagtct gtcgttgcag catcagacgg agaagtggtt tttgccggta
     5461 atgagcttaa gggctacccc aacctcatta ttgtcaaaca cggtgcgcgc tacctcagcg
     5521 cctacagcaa caaccatgct ctgctggtaa aggaagggga caaggtacgt aagggtcagc
     5581 ccattgcaca catgggcaag gtgcctgagg cgacgcgact tcacttcgag atccgcgccg
     5641 acggcaaacc gattgacccg caacaactgc tcccctagcg ctgggcaaaa aaagaattca
     5701 agttattttt ttcttgccga aaacaacaat accggggtga ctagcgacta caacaataat
     5761 cacaacccca ggtgcacaca caacaatacc ggcgaagtaa ccagcggtcg gatagcttcg
     5821 caacccaatt gaggacgtca ccatggcaag cccagcagtc gatttcgata cgctcaaaac
     5881 cgatgatctt catcttatga atgaaattga tgacatcatc acgattgacg acatagcact
     5941 cattgatagc gatgactttg acctggtgcc gagggggcgt gagggggtta tcgaagggga
     6001 agaccccaac ccgaaaaatc tcgatgctac ccaactgtat ttaaatgaaa ttggtttctc
     6061 gcctttatta agcgccgaag aagaagtgtt ttttgcccgc aaagccctca aaggcgataa
     6121 cgcggcccgc aaacgcatga ttgaaagtaa tttgcgcctg gtggtaaaaa tttcccgccg
     6181 ctatgtaaac cgcggtttgg cgctgctgga tttaattgaa gaaggcaatt taggcctgat
     6241 tcgcgcggtg gaaaaatttg acccggaacg cggttttcgc ttttccacct acgccacctg
     6301 gtggattcgc cagaccatcg aacgggcgat catgaatcaa acccgcacta ttcgtttacc
     6361 cattcacgtg gtaaaagaat taaatgtcta cctgcgcgca gcaagagaat tatcgcaaaa
     6421 actcgatcac gagccctccc ccgaagaaat cgccgccctg ctggaaaaac ccgttgaaga
     6481 tgtggagcgc atgctggccc tgaatgagcg cgttacctcc atggacgccc ccattggccc
     6541 ctcctccgat aaaaccctcg tggatacagt gcctgatgta cacgtctccg atccggtcga
     6601 attgctggaa aattccgatt taaatctcag cctggatcat tggctggatg aattaactga
     6661 aaaacaacgg gaagtattat cccgccgctt tggtctgcgc ggccatgaat ccagcacatt
     6721 agaagaagtg ggccgcgaaa ttggtttaac tcgcgagcgc gtgcgccaaa ttcaagtgga
     6781 agccctgcgc cgcctgcgcg atattttgga aaaacaaggc ttaagtgcag aatccttatt
     6841 cggcattatg tagccgctgc actcgcacac tttaattgtt gatggacaat aaaaaacgca
     6901 gcctgttggc tgcgtttttt attggtgaat ttatttttac ctatctctta gctaaaacct
     6961 gtgccccact aaatagtcca tcagccccaa ctatttttat cggcaaaaaa gtaagccccc
     7021 acttaattca caatttaaat tcttccgaca gctaagaaca ctagtgagcg gtttattttc
     7081 aaagccaaat tgcgcgcagc aataaatggt gaactttgct aaaaacggca aaaaagtgtt
     7141 agtgtcgagc gcgatacaag aactgccccg aagccgcata tgctgcagcg cacagaaaat
     7201 actgccattt tgttttttat tagccgcact ttttgcgctg gaaataccgg tctttaatgg
     7261 cgatgtttgc gaaatgagat taaataaaac caagccccag tgccctaagg gatttttaag
     7321 cagcactgtg ctaaaaactg accgcctcac ataataaaaa tctggagccc ccatgacttt
     7381 tgctgtttcc ttccggccct cgccaaggtt tgccttttcc cgccgtttat tatcatccgc
     7441 tgtgctcagc gggcttggcc ttatggcaag cagcgccgag ctattagcca aggcgccgcc
     7501 ccacgaattg ccagccgcga ataaaaccac accggtttta ttacccgctc agtggaatca
     7561 ggtggtaaac cccgtgcccg ccaacccggc actggaagcg aaaattgctg agattctagg
     7621 caaacttacc ctagagcaaa aagttgggca gatgattcag ccccacatag gggcggtgac
     7681 ccccgaagag gtaaaaacct accatatagg ctcaatcctc aatggcggcg gccaaaaccc
     7741 caacgacaac cccaactcca ccgcccagga ctgggcgcaa ctggccgagc ggttttatca
     7801 agcgtctatg tccagtgaca atggccgcac gcccatcccc gtcatctggg gtatagatgc
     7861 cgtgcacggc cacaacaacc tggtgggcgc aacgcttttt ccccacaaca tagccctggg
     7921 cgccactcgc aacccggccc tggttaaatc cctcggcgcc ctcacggctg cggaagttta
     7981 tgccaccggg caggattggg attttgcccc gacccttgct gtagcccagg acgaccgctg
     8041 gggccgcacc tatgaaagtt tttccgaaga gccaagcctg gtagctcaaa tgggcggtaa
     8101 atttgtagaa ggtttgcagg gtacggcggg cactaaggat tttttagcgg cgaaaaaagt
     8161 actggccacg gccaaacatt ttatcggcga tggcggcact caatacggcg atgaccaggg
     8221 ttttaccgag ggggaagaac aggctctgct gcgcacccat ctgcccggtt atatcagcgc
     8281 cctcaacgcc ggtgtgcaaa ccgttatggc cagctacagc agctggaacg gcgtccactc
     8341 ccacgccaat gcccgcttga tgaaccagtt gctcaagggc cagctgggtt ttgatggctt
     8401 tattgtgagc gactggcaag cggtgagcca cgtgcccggc tgttccctgg ataactgccc
     8461 agaggccgtg aacgccgggg ttgacctatt tatgataccc aatgcgccgg actggaaaac
     8521 cttccacgcc aatgtagtgg cccaggtgaa ggccggggtg atcagccaag cacgtattga
     8581 cgatgccgtg cggcgcattc tgcgcgtgaa actgcgccag gggcttttta ccgccccgag
     8641 ccccctgcaa cgggcggcgg caagcagtgg ggtgataggc tctgccgagc accgcgccct
     8701 ggcccgccaa gcggtgcgcg aatccttggt gctgctgaaa aacacccagg gcgtgctgcc
     8761 catcaagcct aaatccaagg tgttggtagc gggcataggc ggcaataatc tgtcccgcca
     8821 aagcggcggc tggagcgtaa cctggcaggg catcgacgtg cccaacgata aattccccgg
     8881 tgccaccagt atttttaaag gcattgccga tgcggtaaaa gccgtcggcg gcagcgctga
     8941 atacagcgag c
//
LOCUS       DAOQGS010000105         8878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3714_length_8878_cov_4.946503, whole genome shotgun sequence.
ACCESSION   DAOQGS010000105 DAOQGS010000000
VERSION     DAOQGS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_8878_cov_4.946503"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3775)
                     /locus_tag="PKZ52_05640"
     CDS             complement(<1..3775)
                     /locus_tag="PKZ52_05640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNG59288.1"
                     /translation="MSKSSRPKPAKPKPAKNTASKQTPVSPLERDVLYSFALAHSLTS
                     TELMRLLQHLKIANKSSAQQLNEAVARLLKLNLLVKTSGYPHKISFGGLSAALADLLF
                     RGGHLPWISALRAVFPIQPRGNPFLQLSTLTCWREYAWATFEGQYEAAIDWRALFNDR
                     NDAQGFPLLGEALLVNGPYGQLLASQPPAIQALLLDEVFARSIATLGPCTHAYDFARA
                     HLTELMAYPDDLEYLCLLALLRADETTFTRLHSALAPWQQLCGQACLSLLKGQYAEAF
                     ALFTEHIKQRKKIHKGTKYLPGIWGYMYCLAALGTNTSESLAALATQVQQGIKQRRAA
                     YNLLEPLVAQLQGGHAIAPIIGSIGSINLNQFSGLIQLLVIIWRQANIGDEGSARLSG
                     HVQSLHTLGYQWLAAEFDAINTLHFKAPAVLPGWHAQRQRLPLFKALPQEETWQRALT
                     ALTKLQRSGSSEGDTRLVWWLRWLSGELAIEPREQKCSAKGVWSKGRPIALKRLYQGD
                     ATAGMSSQDARVIDCIERNRDYYYEEYVLNLAKALPLLVNHPALYWQEAPEVRVELVA
                     GEISLQLQEKPNEIELRLEPSIDSGLPFTLHKITPTRIALYSISSEIKQIASVIGNGL
                     SVPSAAKEQLVAALKAIAPHISIHSDLPELSAQIPSQPADATLYAHLLPLNEGLRLQF
                     LQRPLSEGSWFLPGRGPANVVGEVAGAPIQAVRDLSAEKTHLGQILAACPSLAEAQQM
                     EDQPEWHLEHPEACLELLSELKARPEVTLVWPQGERLRIKGNRSLSNLSLSVKKKGEW
                     FEADGTISLDDGRVMALRELLQLASTSTGRFLKLGESDYLALTNSLRKRLQELNAFAE
                     TTGKGGLRINPLAAPALAELAREVGSLEADDAWRQQVASLDALADFQPKVPSTLQAQL
                     RDYQLQGFQWLARLARWGVGACLADDMGLGKTVQTLALLLSRARGGPALVVAPLSVAM
                     NWLSEAQKFAPTLSMRSHNTQRDLSQLGAFDVVVTSYGLLQQDAEQFAKPHWHTIVLD
                     EAQAIKNANTKRSQAAMALQGDFKMLASGTPVENHLGELWNLFRFINPGLLGSKERFS
                     ERFANPIENGNLDARNHLKKLIQPFILRRTKTQVLSELPPRTEITLQVELSEAEQHLY
                     EALRQQAVDSIATAKAKPLQVLAEITKLRRFCCNPKLVVKNTTIGASKMAVFAETLTE
                     ILANQHKALVFSQFVDHLSLVREYLDAQQIHYQYLDGATSAPERKKRVDNFQAG"
     gene            complement(3974..6100)
                     /locus_tag="PKZ52_05645"
     CDS             complement(3974..6100)
                     /locus_tag="PKZ52_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HNG59289.1"
                     /translation="MLGPIGFFKRQPPLILQTEAAECALACLASISNFYGRACDLMSL
                     RSQFDISLKGSSLSSLIQLAEAMGFNSRPVRAELNELSELQLPAILHWDLNHFVVLTR
                     SSQQSICILDPAYGAKTLPLNQASRHFTGVALELYPKTTYSKPITPQRLGFRTLLHNM
                     LGIKSALASLFSIALLLQLFTLATPIFMQMAVDTVVVSEDKDLLTALGLGFLLLALIQ
                     PIIGSCRQWFAIRLGQATEFHLHNNLYAHLLRLPLAFFEKRQSGDIISRFDSLKQVQT
                     ALTQQFIESILDGLLACLTLLMLALYSIPLTLVVVAAISLYTLLRAITHRPLRAAGEH
                     DLVTAAKKHSYFLESIRAIQSIKTADNIPNRTNRFNNLVVENQNAQLNKQKIALGYHF
                     GNQLIFGLENILVTWIGISAILNHQLSLGVLFAFITYKRQFTTCVTALIDNIFEFKML
                     DLHLQRIGDIALSPTEVNGHQSPSAQALTINLEKVSFRYGFNEPFLFNHLSMHLAAGE
                     TLAIVGPSGTGKSTLVKLLLGLLNPTEGVVLFGQQDLHQLDKTAYRNLVAAVTPEDQL
                     ISGSIRDNIMFGNTQVDETRLLQAAEAAALHTEILAMPMGYHTLVGDMGAVLSSGQKQ
                     RLLIARALYRQPKILILDEATSHLDINNEKKILANIKHLVPSQILIAHRPHTIACADR
                     VMRLTPQGFVYLTTEEVLACAPHTAN"
     gene            complement(6140..7378)
                     /locus_tag="PKZ52_05650"
     CDS             complement(6140..7378)
                     /locus_tag="PKZ52_05650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNG59290.1"
                     /translation="MGQALFRAEVFSAPPLNWGTTRLGAPLPWQLVIGACALIIAASI
                     VLLLRVEYTNKAKVAGFLSPSQGVINLWSPSGGVIRELYVKEGDTVEKGQTLCLISSA
                     TNTATGNTQAQILEQLTNKKHSLEESLNRQDTIADLERTKLTSQSEKLNADWAQNRAE
                     FNLAERKIQSLQLIGDKYQRLYQQQFVSELELEQARQRILEAQLHLHNLTQNAHFLQH
                     EISRMAQEHALLAASSQERKNALQRDINQLQQEILQQQHNSAHVVAAPDAGIVGTVAV
                     HPSQMINANQSLFTLLPAHSPLQAVLIVPSRAAGFIQTGQQVVMRYGAFPYQKFGTQQ
                     GKIISIDHAITQQGDANVPIAIREPSYLVAVELNAQAIHTYGDTTALKAGMLLDADIY
                     LDHRSLADWLLDPIKSISRK"
     gene            complement(7553..8059)
                     /locus_tag="PKZ52_05655"
     CDS             complement(7553..8059)
                     /locus_tag="PKZ52_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59291.1"
                     /translation="MISNETLRTTFITPGIRYYLHKKLAHISPAQVDIRTEELLKFLN
                     ISHHCSGDIPFNHEVDEIWHLWILETQEYFALCQKLRGAPNSYIHHCSNDYIEFSNPE
                     IKAQEPDMDRLFNILLTYVYNYGPFEAERIIYWPLALDIQQNAQLTVDEFNQWLFQVL
                     REQSSLCA"
     gene            complement(8112..8324)
                     /locus_tag="PKZ52_05660"
     CDS             complement(8112..8324)
                     /locus_tag="PKZ52_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59292.1"
                     /translation="MTIRTLDENEIDQVAGGALATTFVASTSSLTSLASSSSFIYRPI
                     KAICKIGGGVGPISCMEVIPAGVVAR"
BASE COUNT         1960 a         2051 c         2543 g         2324 t
ORIGIN      
        1 ctccggcttg aaaattatcc acgcgttttt tgcgctcggg ggcgctggtg gcaccatcta
       61 aatattggta gtgaatctgc tgtgcatcga gatactcgcg caccaaactt aagtgatcaa
      121 caaattggct aaatactagc gctttatgtt ggttcgctaa aatctcagtt agcgtctcgg
      181 caaatacggc cattttgctg gcgccaatcg tggtgttttt taccaccaat ttagggttgc
      241 agcaaaacct gcgcagcttg gtaatttctg ccaacacttg caggggtttg gcttttgcgg
      301 tggcgatgct atcgacggcc tgttggcgca gggcttcata caggtgttgt tcggcttcac
      361 ttaactctac ttgcaaagta atttccgtgc gcgggggtaa ttcgctgagc acttgggttt
      421 tggtgcggcg caaaataaag ggttgaatta attttttgag atggttgcgt gcatccaggt
      481 tgccgttttc aatggggttg gcaaaacgct cgctgaagcg ctctttggag ccgagcaggc
      541 caggatttat aaaacgaaat aaattccata attcccctag atgattttcc accggggtgc
      601 cgctggcgag cattttaaaa tccccctgta aggccatggc cgcttggctg cgtttggtgt
      661 tggcattttt gatggcttgc gcctcgtcca acacaatggt gtgccagtgg ggcttggcaa
      721 attgttcggc atcctgttgc agcaggccat aactggtgac gactacatca aaggcgccca
      781 gttggctgag gtcgcgctgg gtgttgtggc tgcgcatact gagggtgggg gcaaattttt
      841 gcgcttcgct cagccagttc atggccacgg acagaggcgc aaccaccaga gccgggccgc
      901 cgcgggcacg gcttagcaat agggcgaggg tttgcacggt tttgcccagc cccatgtcgt
      961 ccgccaggca ggcgcccacg ccccagcggg cgaggcgggc cagccattga aagccttgca
     1021 gttggtagtc gcgcagctgg gcctgcaagg tgctgggcac tttgggctgg aagtcagcca
     1081 gggcgtcaag gcttgccacc tgttggcgcc aggcatcgtc ggcttccagc gagcccactt
     1141 cgcgggctag ttccgccagg gcgggcgcgg ctaaggggtt gatgcgcaag ccgcccttgc
     1201 cggtggtttc ggcgaaggcg tttaactcct gcaggcgttt gcgcaggcta ttggtgaggg
     1261 ccaaataatc gctctcgcca agttttaaaa agcgcccggt gctggtgctg gccagttgca
     1321 acagttcgcg cagggccatc acacggccgt cgtccaggct gatggtgccg tcggcttcga
     1381 accattcgcc ttttttcttc acgcttaaac taaggttgct aaggctgcgg ttgcccttga
     1441 tacgcaggcg ctcgccctgt ggccatacga gggtcacttc gggccgggct ttcagctcac
     1501 ttaataattc caggcaggcc tctgggtgtt ccaggtgcca ttctggttga tcctccattt
     1561 gctgggcttc ggcaaggctt gggcaggcgg ccagaatctg cccgaggtgg gttttttccg
     1621 cgcttaaatc ccgcactgct tgaattgggg cgccggcaac ttcacccacc acattggcgg
     1681 ggccgcgccc agggagaaac caactgccct cgctgagagg ccgttgcaaa aattgcaggc
     1741 gcaggccctc gttgaggggc aataggtgcg cgtacagggt cgcatcggct ggctggctgg
     1801 ggatttgggc ggagagttcc ggcaagtcgg agtgaatgct gatgtggggt gctattgcct
     1861 tgagagcggc aaccaattgc tctttggcgg cactagggac actcaagcca ttgccgatga
     1921 cgctggcgat ttgttttatt tcactgctga tgctgtagag ggcaatgcgg gtgggggtta
     1981 ttttgtgcag ggtaaatggg agtccgctgt caatggaagg ctctaagcgc agctcaattt
     2041 cgttgggctt ctcctgcagt tgtaggctga tttcaccggc taccaactcc acccgcacct
     2101 ccggtgcctc ctgccaatac agggcggggt ggttgaccaa tagcggtagc gctttggcga
     2161 ggttgagcac gtactcttcg tagtaataat cgcggttgcg ttcgatgcaa tcgatcaccc
     2221 tggcatcctg gctgctcata cccgctgttg catccccttg gtacaggcgt ttaagggcga
     2281 tggggcggcc tttgctccaa acacccttgg cactgcattt ttgctcccgt ggctctatgg
     2341 ctaattcgcc gctgagccag cgcagccacc ataccaggcg ggtatctcct tcggagctgc
     2401 cgctgcgctg caatttggtg agggcggtta gggcgcgctg ccaggtttcc tcttgaggta
     2461 gagccttaaa taggggtaag cgctgccgct gtgcatgcca gccgggtaat acggctggcg
     2521 ccttgaaatg cagggtgttt atggcatcga attcggctgc cagccattgg tagccgagtg
     2581 tgtgcagcga ttgcacgtgc ccacttaagc gcgcactgcc ctcatcgccg atattggctt
     2641 ggcgccagat gattactagc agttggatca agccggaaaa ctgattgaga ttgatgctac
     2701 ctatgctgcc gatgatgggc gctatggcgt ggccgccttg caactgggca actagcggct
     2761 ccagtaggtt gtaggcggct cggcgctgtt tgataccctg ttgcacctgg gtggccaggg
     2821 cggccaggct ttcggaggtg ttagtaccca gtgcggccaa gcaatacata tagccccaaa
     2881 tgcctggcag gtatttagtg cctttgtgga tttttttgcg ttgttttatg tgctcagtaa
     2941 acagcgcaaa tgcctcggca tactggcctt ttagcaggct caaacacgcc tgcccgcaca
     3001 gctgttgcca aggcgcgagc gcactgtgga gcctggtgaa ggtggtttcg tccgctctta
     3061 acaaggctag caagcacaag tattccagat cgtctgggta ggccatgagc tctgtcaggt
     3121 gggcccttgc aaagtcatag gcatgtgtac aggggccaag tgtggcgatg ctgcgggcaa
     3181 acacttcatc caacagtaag gcttggattg ccggcggctg cgacgccaag agttggccat
     3241 aagggccgtt gaccaggagg gcctcgccta gaagcggaaa gccctgcgca tcatttcggt
     3301 cattgaatag agcgcgccaa tcgattgccg cctcgtattg gccttcaaat gtcgcccagg
     3361 cgtattcgcg ccaacaggtc agcgtgctga gctgcagaaa ggggttgccc cgcggctgta
     3421 tcggaaatac cgcgcgcaat gcgctgatcc aaggcaaatg gccgccccga aaaagcagat
     3481 ccgcaagggc ggccgacaaa ccgccaaagg aaattttgtg gggatagccg ctggttttaa
     3541 ctaaaaggtt taacttcagc agccgcgcga cagcctcatt aagctgttgc gccgaacttt
     3601 tgttagcaat cttgaggtgt tgcaacaacc tcattaactc ggtgctggtg aggctgtggg
     3661 ccagtgcgaa gctatacagt acatcgcgtt ccagggggga gacgggggtt tgtttgctgg
     3721 ctgtgttttt agcgggttta ggttttgcag gcttaggcct ggacgattta gacatggtat
     3781 ggcggcagcg ctgggagaga agagagtgca ggataaagat atttagtggc tccatcaaaa
     3841 atataaccgc gcctggggtt tggcctttga attattaacc tggcggcaaa atagatcgtc
     3901 ctgatctatt ttgccgccgg gttaatagga cctaattgct gcgtgtctgc aatcatgtgt
     3961 cttcattgcg acttcagttc gccgtgtgag gcgcgcaggc gaggacctcc tctgtggtga
     4021 gatatacaaa tccctgaggc gtaaggcgca tgacgcggtc ggcgcaggca atggtgtggg
     4081 ggcggtgggc gattaatatt tggctgggca ctaggtgttt aatattggcg agtatttttt
     4141 tctcgttgtt gatatccagg tggctggtgg cttcatccag aatcaggatt ttgggctggc
     4201 ggtacagcgc gcgggcgatc agtagccgtt gtttttgccc gctggataac actgcgccca
     4261 tatcgcccac cagcgtgtga tagcccatgg gcatggcgag aatttccgtg tgtagggccg
     4321 ccgcttcggc tgcctgtaat agccgtgttt cgtccacctg ggtattgcca aacataatat
     4381 tgtcgcgaat ggagccgctg atgagttgat cttccggtgt cacggcggct actaaattgc
     4441 ggtaggcggt tttatccagc tggtgtaaat cctgttggcc aaataaaaca acgccctctg
     4501 tgggatttaa taagcctaat aataatttta ccagcgtgga tttacctgtg ccagacgggc
     4561 cgacaatggc tagggtttcc ccggcggcta gatgcatgga taagtgatta aataaaaacg
     4621 gctcgttaaa cccgtagcga aaacttacct tctcaaggtt tattgtcaat gcctgagcgc
     4681 tggggctttg gtggccgttc acttctgtgg ggctcaaggc aatatcgcca atgcgctgca
     4741 ggtgtaaatc cagcatttta aactcaaaaa tattgtcgat taaggccgtt acgcaggtag
     4801 tgaattggcg tttataggtg ataaacgcaa acagcacacc gagggacaat tggtggttga
     4861 gaatagcgct gataccgatc caggtgacga gaatattttc caggccaaaa atcaactgat
     4921 tgccaaagtg atagcccagg gctatttttt gtttattcag ctgggcattt tgattttcca
     4981 ccacaagatt attaaaccgg ttggtgcgat tggggatgtt gtcggcggtt ttaatgcttt
     5041 gaatggcgcg gatgctctct aaaaaatagg agtgtttttt ggcggcggtc accaaatcgt
     5101 gctcacctgc agcacgcagt ggccggtggg tgatggcgcg cagcagtgtg taaaggctta
     5161 tggcagcaac cactaccagg gttagcggga tgctgtacag cgctagcatc agcaaggtga
     5221 ggcaggctaa taagccatct aaaatacttt cgataaattg ttgggtgagg gcggtttgca
     5281 cctgtttgag agaatcaaag cgggaaataa tatcgccgct ttggcgtttt tcaaaaaatg
     5341 ctaggggcag gcgcaatagg tgagcgtata aattattgtg caggtgaaat tcggtggctt
     5401 ggcctaagcg aatagcgaac cactggcggc agctaccgat aatgggttgt atgagtgcta
     5461 ataataaaaa acctaaaccc agcgcggtta ataaatcctt atcttcagat accacaacgg
     5521 tatccactgc catttgcata aaaatgggtg tggccagggt gaataactgc agcagcaggg
     5581 caatggaaaa taaactggct aaggcggatt taatgcccag catattgtgc agcaaagtgc
     5641 gaaaccccaa gcgctgcggt gtaatgggtt tgctgtacgt ggttttaggg tatagctcca
     5701 aggcaacgcc ggtaaaatgg cgggatgcct ggttgagggg aagagttttt gcgccgtacg
     5761 cggggtcgag aatacagata ctttgttggc tgctgcgggt gagcacgaca aaatggttga
     5821 gatcccagtg caaaatggcg ggcagctgta attcgctcaa ttcgttcagc tcggcccgca
     5881 ccggccgcga gttaaaaccc atcgcctcgg ccaattgaat taagctgctg aggctggagc
     5941 cttttaggga gatatcaaat tggctgcgca ggctcattaa atcgcaggcg cggccataaa
     6001 aattgctaat actggctaaa caggccagcg cgcattcggc cgcttcggtt tgcaaaatga
     6061 gcggcggctg ccgtttaaaa aatcctattg gccctaacat aaataaatcc tgatgggttt
     6121 taaaatatga accgatgcgt tatttgcggc tgatgctttt gatcgggtcg agtagccagt
     6181 ctgccaagga gcgatggtct aaataaatat cggcgtccaa taacatgccg gcttttaatg
     6241 ctgttgtgtc accataggtg tgaatagcct gtgcgtttaa ttccaccgcc actaaatagc
     6301 tgggctctcg aatggcaatg ggcacattgg catccccctg ttgggtaata gcgtgatcga
     6361 tggagataat tttgccctgt tgagtgccga atttttggta gggaaaggcg ccgtaccgca
     6421 tgacaacctg ttggcctgtt tgaatgaaac ccgcggctcg gctgggcaca attaacacgg
     6481 cttgcaatgg gctgtgtgcg ggcagaagag taaataggct ctggttggca ttaatcatct
     6541 ggcttgggtg cacagctaca gtgccgacaa ttccagcatc cggtgcagct accacatggg
     6601 cgctgttgtg ttgctgctgg agtatttcct gttgtagctg attgatatcg cgctgcagag
     6661 catttttgcg ctcttggctg ctggctgcta ataatgcatg ctcttgcgcc atacggctta
     6721 tttcatgttg taagaaatga gcgttctggg tgaggttgtg caggtgtaat tgtgcctcca
     6781 aaattctctg gcgcgcctgc tccagttcta attcggagac aaattgttgt tgatataggc
     6841 gctggtattt atcgcctatg agttgcaagg actgtatttt acgttcggcc aggttaaatt
     6901 cggcgcgatt ttgcgcccag tcggcattga gtttttcact ctgggatgta agttttgtgc
     6961 gctccagatc ggcaatggta tcctggcggt ttaacgattc ttctaggctg tgttttttat
     7021 tggttaattg ttcgagaatt tgcgcttggg tgttgccggt ggcggtgttg gtcgcgctgc
     7081 taattaaaca cagggtttgc cccttctcta cagtatcgcc ttcttttaca tacaactcac
     7141 gaataacgcc gccgctgggt gaccagaggt ttatcacacc ctggctgggg gataaaaacc
     7201 cggccacttt ggctttgttt gtgtattcta cgcgcaggag taaaacgata ctcgcggcaa
     7261 taatcagtgc gcaggcgcca attaccagct gccagggcag gggggcgcca aggcgtgtgg
     7321 tgccccaatt caacggcggg gcgctgaata cttctgcgcg aaataatgct tggcccataa
     7381 atattcctta gatcctgcgc cacagggcgc gtgtagtgac actgcatgta catgctggat
     7441 tttgattaat tgatgttttc aaaaacccat gcgtacattt ctttaacgcc taagtaggct
     7501 tgtcttctgc gttggttttg aaatctctac ttattcagcg cgtccactat tcttacgcgc
     7561 aaagcgatga ttgctcgcgc agtacctgga ataaccactg attaaattca tccaccgtca
     7621 actgtgcatt ttgttggata tccaacgcta gcggccaata aataattcgc tccgcctcga
     7681 atggcccata gttgtagacg taggttaata gaatattaaa taggcggtcc atgtctggct
     7741 cctgtgcttt aatctcagga ttggaaaact ctatataatc attggagcag tgatggatgt
     7801 aactgttagg tgcgcccctt aatttttggc agagggcgaa atactcttgg gtttccaaaa
     7861 tccataaatg ccaaatttcg tctacttcat ggttaaacgg aatatcgccg ctgcagtggt
     7921 gggatatgtt gagaaactta agtaactcct ctgtgcgtat atcaacctgt gctgggctaa
     7981 tgtgggccag ttttttatgc aaataatagc gaatgcccgg cgtgataaag gttgtgcgca
     8041 gagtttcgtt gctaatcata tcgcctcctt aacaaggcct tattaacttt gaattaataa
     8101 ggcctatcgg gttatcgagc gacaacgccc gcaggaataa cttccatgca gctgataggg
     8161 cctacgccgc cgccaatttt gcagattgct tttatcggcc gataaataaa gctggatgag
     8221 gaagctaaag aagtgaggga gctggtgctg gcaacaaaag ttgtcgccaa ggcgccgcct
     8281 gcgacttggt cgatttcatt ttcatctaaa gtgcgaatag tcataaagat tctcctaaaa
     8341 gtatttgatt gagcttaatc gagaaaagtc ggttaagtgt tagcaggtta tgagtaaccc
     8401 gaaccctaag ctgtgtgata ttgccggttt attctgcgca aaagttacag ccctggctga
     8461 gggaatagct gtaggctggt aaatttttta tgcgggtgat ttatttgtta aaagtgaata
     8521 actgtagatt ttgcaaatgt cgataggttt aacaggtgcg ggcgcgcata tggggaggca
     8581 attatcgcaa gttgattgga ctcaatctgt agggatgctg gtggttattt ttaaataatc
     8641 ctgcgaaaag ggatttccta ataaaggatg ttctgattaa gcctgaattc ctgactgttt
     8701 tgaatatgta caagtatcct attttgcccc gtcgctgggc tgcctgctgg cgaaggttgc
     8761 acaatatccc tgacttaaaa agaggtttct aaaatgcacc tatatcatcc ggctggtctg
     8821 ctctagcagc ttgttgaaat actaccttgc tagcactggc ggcgttaaat ttggcgaa
//
LOCUS       DAOQGS010000106         8870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3721_length_8870_cov_4.363018, whole genome shotgun sequence.
ACCESSION   DAOQGS010000106 DAOQGS010000000
VERSION     DAOQGS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_8870_cov_4.363018"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1016
                     /locus_tag="PKZ52_05665"
     CDS             6..1016
                     /locus_tag="PKZ52_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HNG59293.1"
                     /translation="MDGCEPNCVPDEVYASGQPVVLKGLVKHWPLVCAALESPAAAVA
                     MLKEHYSGQLAPVYFGDPGVRRWAYNPEVSQLNFAIKKANIAEVLDEILDGLQQESPP
                     VRYIASNIIDTYFPSLIEQNDLPFNSSYFNEYPLEFADPLRGIWIGNKTNSPCHYDAQ
                     SNIACCIAGRRKFTLFPPDQVHNLYPGPLDLNPGGPAITMVDFDEPDFERFPRFAEAI
                     KFGQVAHLEPGDAIFIPCMWWHQVEALSPFNILINYWWNTYPKIRGQAMNVLYHALLS
                     IRDRPEQEKQAWKHLFDYYVFNSDSNAGAHLPEHARGYLGAIDDNAARRLRAMLLQKL
                     NR"
     gene            1039..2514
                     /locus_tag="PKZ52_05670"
     CDS             1039..2514
                     /locus_tag="PKZ52_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HNG59294.1"
                     /translation="MKTQIKKVVIAGGGTAGWIAAVALGRKMGRLLDITLIESDEIGT
                     IGVGEATIPPMRTFHRLLGIDEREFMRATEATFKLGIQFENWGQKGDNYFHSFGKTGQ
                     ETWLCDFHHFWLRAKELGIAGDFGEYCVELQAGKQHRFATAPDAEINYAFHLDAGRYA
                     QYLRGLSEAHGVKRKEGKIQQVLQDDGGYISGLKLASGELIEGDLFIDCTGFRGLLIE
                     QTLNAGYEDWSHWLPCDRAVAVQTASVRPAVPYTRSIAHDVGWQWQIPLQHRVGNGLV
                     YSSPYLSDDEARQKLLDNIEGELLTEPRTIKFQTGRRKEVWKKNCVALGLASGFIEPL
                     ESTSIHLIMMGVNRLMHLFPFSGVNEALISEYNRLSRVEMEQLRDFIVLHYNTTERCD
                     TAFWDYCRAMPVPETLKLRVELFKQNAHAYQADGELFRVDSWTQVMFGQRLMPAHYHE
                     LVKLMPEAELKKFLTTMSANVKNAVEKLPAHADFVRAYCGK"
     gene            2538..3266
                     /locus_tag="PKZ52_05675"
     CDS             2538..3266
                     /locus_tag="PKZ52_05675"
                     /inference="COORDINATES: protein motif:HMM:NF039455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HNG59295.1"
                     /translation="MAFTPQLHADFAYKAYKVGREKTPLLVIDNFIAHAEQLVDYCAA
                     LGKLEKIDTFYPGIRMQAPDMYVHTLYQYLGVLLEHAFDLPLNQIQGSKALFSMVTTP
                     PEHLSPKQRLPHIDSFETGDLACVHYLCSPEMGGTALYRHKSTGFETIDASQIDHYNE
                     TLIKEGALQSEDITYMHGANKYFDQIADIGAVFNRLIIYPSNILHSGTISPNCVLDAD
                     PRKGRLTLNTFVYRKRSRVGGAAS"
     gene            complement(3304..4143)
                     /locus_tag="PKZ52_05680"
     CDS             complement(3304..4143)
                     /locus_tag="PKZ52_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNG59296.1"
                     /translation="MLEIGRFNFLTIKKQVEFGVYLDGGEAGEILLPRRYVPQNAAIG
                     DSLNVFIYFDSEDCLIATTEKPKILCGQCALLTVKDVNKVGAFMDWGLMKDLLVPYAE
                     QHKPFELGQKYVVTAYTDKHTGRILASSKLHRHLKETAHHFPPLTPCDLLVFGKTDMG
                     YKVVVDNAYLGLIFRDEAFKPLRYGQRLNGYIKGSRPDGKLDILLQLPAGIGRDELSQ
                     KILAYIEAQGGVSFITDKSPPEDIYTLFNVSKANFKKAVGGLLKQGKIRLGENEIRLN
                     TQQ"
     gene            complement(4219..6585)
                     /locus_tag="PKZ52_05685"
     CDS             complement(4219..6585)
                     /locus_tag="PKZ52_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNG59297.1"
                     /translation="MQFPPCYHCGLESHPDYSAPINNQPHAFCCLGCQAVALTIAGSG
                     LADFYRFRSEPNNRAKTAKMNFAAFDDAEVQKEFVVELADGAKQANLIIAGITCAACV
                     WLIERHLKSLPGILSCGVNSLNHRAFIRYAPAEIALSDIFIALNAIGYNPEPQRAQSQ
                     FNYWREQQRTALIRLGVAGISMMQAGMVAVGLYAGALQGLDEHWQTILRWITCLFAIP
                     VVFYSSQPFYAGAWRALSLKKLNMDVSVSLALLIAFFASFYASVTQTGEVYFESIAMF
                     AFILLAGRYIEQRARFRNFQQGVNGTHTIPLAALRVNETQTLEELIPVSRVQTGDHIK
                     VTAGELFPCDGTVVSGESYAIESLISGESQPQPKKPGSQVLAGSINGDAALVIAVTAT
                     GPNTQLAAIEQLMDEASANRPRYLSFNDRISNYFIAAVLVVAAGSFTAWHFIAPAKAL
                     WIALSVLVVTCPCALAIAMPAAWICALNHLRQKGVLIKSSDFFERINKVTRIVFDKTG
                     TLTDGELTLAQVIALQGTQEQALSVIASLEAASVHPIAQAFLNIPSAGRVDNNRVVAN
                     QGVEGNIQGIFYRFGAAHFAAPACPPAYPGVGQWLLLTADGAPMAWVQLQDKIRPQAK
                     TCLEQLTQAGYTCEIISGDRQENVEQLASQLGVEQWQANATPQDKLTRLLQYQQNQAV
                     MMVGDGINDVPVLAGADASLAMGRASTLAQTQAQAVLIQSDLALINYLFHYAARLQHI
                     IKQNFYWALAYNCIAIPAAVMGWVPPWLAAVGMSTSSLIVIMNSLRLA"
     gene            complement(6589..7128)
                     /locus_tag="PKZ52_05690"
     CDS             complement(6589..7128)
                     /locus_tag="PKZ52_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNG59298.1"
                     /translation="MINPAAVEASVAGKKSSWREPWFWMVLGPLILTILTSLIFVYIA
                     VSGADQRVVDDYYTEGKAINHRFEADKLAAALGVGAQVRVDTVSGEALVELTGLSVFP
                     DQLTLYFSHPVKATDDLTVQLKKINASRYRVDLPKQLAGRWYLQLTPADAAADKKWRL
                     VGELDLNQQQTLTLAPEVQ"
     gene            complement(7121..8545)
                     /gene="ccoG"
                     /locus_tag="PKZ52_05695"
     CDS             complement(7121..8545)
                     /gene="ccoG"
                     /locus_tag="PKZ52_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNG59299.1"
                     /translation="MTDKPQGDKPEIRYVNLYEPEAKIYTRKISGFYQRLRRYSGIPL
                     VALFVLLPWISIDGRPAVWLDMAHQKFHFLSITFWPQDLMLLAWLFIIAAYALFTVST
                     VFGRVWCGFTCPQTIWTLIFIVIEDLCEGDRNKRIKLDKQSWDGEKILRKGSKHLLWF
                     LVAFFTGITFVGYFVPAPELVKGLLPHITPDGHIGIELGGWPIFFVLLCSGMTYLNAG
                     WMREQVCKYMCPYSRFQAAMYDKDTIYVQYDSARGEQRGPRKPNEDYKAKGLGDCTDC
                     SWCVQVCPVNIDIRNGTQWECINCGLCVDACDSVMDKMGYERGLVRFTSETQLTTGKY
                     RIIRPKTIGYAVAMLIMVAAFSYRLSIREPLSVDVVRDRSAHLFRLDGDDIQNVYTVK
                     INNMDQQEHTFVVDVKGLYSFRVLNARQVHVKSGEVYSLPVRVAVPKAELKGEENTIT
                     ISATSVETGLSASEDSTFMGPSHD"
     gene            complement(8607..>8870)
                     /locus_tag="PKZ52_05700"
     CDS             complement(8607..>8870)
                     /locus_tag="PKZ52_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNG59300.1"
                     /translation="AQAELGAKVFAENCVACHGTDAKGQYATGAPNLTDKTWLYEGTR
                     ENIRQTIRNGRNNQMPAQEHTLLPEKIHLLAAYVYHLSNNAAK"
BASE COUNT         2204 a         1989 c         2371 g         2306 t
ORIGIN      
        1 aaaccattga cggatgcgag cctaattgtg tccccgatga ggtctatgca tctggccaac
       61 cggtcgtatt aaaagggctt gttaaacatt ggccgcttgt atgtgctgcc cttgaatcgc
      121 cagctgctgc agtggcaatg ctaaaagagc actatagtgg acagttagcg ccggtttact
      181 ttggggatcc gggggttaga cgttgggctt acaacccgga agttagccaa ctaaatttcg
      241 cgatcaaaaa agcgaacatc gcagaagtgc ttgatgaaat attggatggg cttcagcagg
      301 agagcccgcc ggtccgatat attgcctcaa atataattga tacctatttt ccatcattga
      361 tcgagcaaaa tgatttgcct ttcaattcct cttactttaa tgagtatcca ctagagtttg
      421 ccgatccgtt acgaggaatc tggattggta ataagactaa ctcgccatgc cattacgatg
      481 cccagagcaa tatcgcttgt tgtattgcgg ggcggcgcaa gtttactttg tttccaccgg
      541 atcaggtgca taatctttac cccgggccgc ttgatttaaa tccgggtggc ccggccatta
      601 caatggttga ctttgatgag cccgatttcg agcgatttcc gcgttttgcg gaggcaatta
      661 aattcggtca ggtcgctcat ttagaacctg gcgacgcgat atttattcct tgtatgtggt
      721 ggcatcaggt tgaagcgtta agccctttta atattttaat taattattgg tggaatacct
      781 acccaaaaat ccgtggccag gcgatgaatg ttctctatca cgcattgttg agtattcgtg
      841 atcggccaga gcaggagaag caggcttgga agcacctatt tgattactat gtatttaata
      901 gtgatagcaa cgccggtgcg catttgcctg aacatgcgcg tggttatttg ggggctatag
      961 atgataatgc ggcgcgtcga ttgcgagcga tgttattgca gaaactaaat cgttaattta
     1021 attgcttgga aaaaaatttt gaaaacgcaa attaaaaaag ttgttatcgc aggtggcggc
     1081 acggcgggtt ggattgctgc ggttgcgctt ggccggaaaa tgggacgctt gttggatatc
     1141 acactaatcg agtcagacga aattggcact attggtgttg gtgaggcgac cattccacct
     1201 atgcgcactt ttcatcgctt gcttggaatt gatgagcgtg aatttatgcg ggcaaccgag
     1261 gctactttta agttggggat ccaatttgaa aattgggggc agaagggtga taactacttt
     1321 cactcctttg gtaaaaccgg ccaagagacc tggctgtgtg atttccacca tttttggttg
     1381 cgcgcgaaag agttgggtat cgctggggat tttggtgaat attgtgttga gctgcaggca
     1441 ggcaaacaac atcgttttgc caccgctccc gatgcagaaa tcaattacgc attccatttg
     1501 gatgcgggtc gttatgcgca atatctgcgt ggtttaagcg aagcacatgg tgttaagcgt
     1561 aaagagggaa aaatacagca ggtcttacaa gatgatggcg gttatatttc cggtctaaaa
     1621 ctagcatcag gagaattaat agagggggat ttgtttatag attgcaccgg atttcgcggc
     1681 ctattgattg agcagacgtt aaacgcaggc tatgaggatt ggtcccattg gttgccgtgc
     1741 gatcgcgctg tggctgtgca gacagcaagt gtaaggcctg ctgtgcccta tacccgttcc
     1801 attgcacatg atgtgggttg gcagtggcaa attccattgc agcaccgtgt aggcaacgga
     1861 ctggtatatt caagtcctta cttatccgac gatgaggcca ggcaaaaact cctcgataat
     1921 atagaggggg agttgcttac cgagccgcgg actatcaagt tccaaacagg acgacgcaaa
     1981 gaggtgtgga agaaaaactg cgtcgcactc gggctggcga gtggctttat tgagccgtta
     2041 gagtcaacca gcatccattt gattatgatg ggtgttaata ggctgatgca tttatttcct
     2101 ttttcaggtg tgaatgaagc gttaatctcg gaatacaatc gtttgagccg ggttgaaatg
     2161 gagcagctgc gtgattttat tgtgctgcat tataatacta ctgaacgttg tgacactgcg
     2221 ttttgggatt attgtcgggc catgccggtg cctgagaccc taaagcttag ggttgaactc
     2281 tttaaacaaa atgcccacgc ctatcaagct gacggggaat tatttcgtgt ggattcgtgg
     2341 acgcaggtta tgtttgggca gaggttaatg ccggcccatt atcacgagtt ggttaaatta
     2401 atgcccgagg ccgagttgaa gaaattccta acaactatga gcgcaaatgt gaaaaatgca
     2461 gttgaaaaac ttcctgcaca cgccgatttt gtgcgggcct attgcgggaa ataaggttac
     2521 attttggggg gtaaataatg gcttttacac cgcaactgca tgctgatttt gcctataagg
     2581 cctataaggt gggcagggaa aaaacccctt tgttagttat tgataacttt atagcacatg
     2641 cagagcaatt ggtggactat tgcgcggctc ttggaaagct ggagaaaatc gatacctttt
     2701 acccaggcat tagaatgcag gcgccagata tgtatgtgca caccttgtat caatacctgg
     2761 gtgtactctt agaacatgcg tttgatttgc cgctcaatca aattcaggga agtaaagcgc
     2821 tattttcgat ggtgacgacg ccacctgagc atctgtctcc caagcagcgg ttgccacata
     2881 tcgattcatt tgaaacgggt gacctggcgt gtgtgcatta cctgtgttcg cccgagatgg
     2941 gtgggacagc tttgtatcgc cataaaagca ctggtttcga gacgatcgat gcaagccaaa
     3001 tagaccatta taatgaaacc ctcataaagg aaggcgcttt acagtcggag gacataacct
     3061 atatgcatgg ggcaaataaa tactttgatc aaattgcgga tataggtgct gtatttaatc
     3121 gccttattat ttacccctca aatattcttc attcgggcac cataagtcca aattgtgtgc
     3181 tggacgcaga ccctcgtaaa gggcgcctta ctttaaatac atttgtttac agaaaaagat
     3241 cacgagttgg tggtgccgcg agctagcaat ttccaaaatt gtccagaggc atgagcctat
     3301 atgttattgt tgagtattta atcggatttc gttttctccc aatcttattt tcccttgttt
     3361 taataagccc ccgaccgcct ttttgaaatt tgctttgctg acattgaata atgtgtaaat
     3421 atcctccggc gggcttttgt cggtgatgaa tgatactcct ccttgagcct cgatataggc
     3481 tagtattttt tggctcaact catcgcgccc gatgcctgcg ggcagctgta atagaatatc
     3541 cagctttcca tccggtcgcg aacctttaat atagccgttt aaacgctgcc cgtagcgcaa
     3601 aggtttaaat gcctcgtcgc gaaaaattag gcccaaataa gcattgtcca cgactacttt
     3661 gtagcccatg tctgttttgc caaaaactaa taaatcacat ggggtgaggg gtgggaagtg
     3721 gtgtgcggtt tcttttaaat ggcggtgcaa ttttgaggat gccaaaatgc gcccggtatg
     3781 tttatctgtg tacgcggtaa caacgtattt ttgccccagc tcgaaaggtt tatgttgttc
     3841 tgcatagggc accaagagat ctttcattag gccccaatcc ataaaagcgc ccactttgtt
     3901 aacatccttt accgttaata acgcacattg cccgcaaaga attttgggtt tttctgtggt
     3961 ggcgatgagg cagtcttcgg agtcaaaata aataaataca tttaagctgt cgccaatggc
     4021 ggcattctgg ggaacatatc ggcgcggcaa taaaatttcg ccagcttcgc cgccgtcgag
     4081 gtaaacacca aattccactt gtttcttgat tgttaagaag ttgaagcggc caatttctag
     4141 cataaaaatc tacgtgtgag gcaaaaaacc agtctaactc aggcagtgaa aaaagtaact
     4201 gtgcgtggtg cttgggggtt acgccaggcg cagagaattc atgatgacaa ttaatgagct
     4261 ggtagacatg ccgacggcag ccaaccaggg tggcacccat cccattacgg cagcgggtat
     4321 ggcgatgcag ttgtacgcga gagcccagta gaaattctgc ttaatgatgt gttgtaggcg
     4381 ggcggcgtag tggaagagat aattgataag ggcgagatcg ctttgaatga gtactgcttg
     4441 agcttgggtt tgtgcgaggg tactggcgcg gcccatggct aaggaggcat ctgcaccggc
     4501 taatacaggc acatcgttaa tgccatcgcc caccatcatg actgcctggt tttgttggta
     4561 ctggagcagg cgggttaatt tatcctgagg cgtggcgtta gcctgccact gctctacacc
     4621 taactgggat gcaagctgct cgacattttc ttggcgatca ccggagataa tttcacaggt
     4681 gtagcccgct tgcgttaatt gttcgagaca ggtttttgcc tgggggcgga ttttatcctg
     4741 caattgaacc caggccatgg gggcgccatc ggcagttaat aataaccatt ggcccacacc
     4801 ggggtaggcg ggggggcagg caggggcggc gaagtgggcg gcaccaaagc gataaaaaat
     4861 gccctgaata ttaccttcaa caccttgatt ggcgacaact ctattgttgt ctacacggcc
     4921 ggcactgggg atgtttaaaa atgcctgtgc aattgggtgc acactcgcag cctccaagct
     4981 tgcgattacg ctgagcgcct gctcctgcgt gccttgcaac gcgataactt gggcgagggt
     5041 taattcgcca tcggttaggg tgccggtttt atcaaacacg atacgcgtga ctttattaat
     5101 gcgctcaaaa aaatcactgg atttaattaa cacgcctttt tggcgcaggt ggtttagcgc
     5161 acaaatccag gcggcgggca tggcgatggc aagtgcgcag ggacaggtca ccaccaatac
     5221 tgatagggca atccagaggg cttttgcggg ggcaataaaa tgccaggcgg taaaactgcc
     5281 tgccgctacg actaaaacgg cggcgataaa ataattgctg atgcgatcat taaacgacaa
     5341 ataacgtggg cgattagcgc tcgcctcatc cataagttgt tctatcgcag ctaattgtgt
     5401 attggggcct gtggctgtaa cggcaataac gagcgcggca tcgccattga tactgccagc
     5461 taacacttgg ctgccgggtt ttttcggttg cggttgtgat tcgccgctaa tgagcgattc
     5521 aatagcatag ctttcgccgg ataccactgt gccgtcgcag ggaaataatt cacctgccgt
     5581 cactttaata tgatcgcctg tttgcacccg cgatacgggt ataagttctt cgagtgtttg
     5641 ggtttcattt acgcgcaatg cagctagggg tatggtgtgc gtgccgttga cgccctgttg
     5701 aaaattgcga aaccgagcgc gctgttcgat ataacggccc gccaataaaa taaaggcaaa
     5761 catggcgatg gattcaaaat acacctcgcc ggtttgcgtg acggatgcat agaaactggc
     5821 aaaaaaggca attaatagag cgaggcttac ggagacatcc atgttgagtt tttttaggct
     5881 cagcgcgcgc caagcgccag cgtaaaacgg ttgcgatgaa taaaacacta cgggtatggc
     5941 gaataaacaa gtaatccagc gcaaaatagt ttgccagtgt tcatctaacc cctgtagcgc
     6001 acccgcgtac aggcccacgg ccaccatgcc cgcctgcatc atgctaatac cggcaacccc
     6061 caggcgaatt aaggctgtgc gttgttgttc gcgccaataa ttaaattggc tttgggcgcg
     6121 ttggggttcg gggttgtagc ctatggcatt gagtgcgata aaaatatcgc tgagggcaat
     6181 ttctgccggg gcgtatcgaa taaatgcgcg gtggttgagt gagttgacgc cgcagctgag
     6241 gataccgggc agggatttta agtggcgttc aattaaccac acgcaggctg cgcaggtgat
     6301 gcctgcaata atgaggttgg cttgtttggc gccatcggca agctccacca caaattcttt
     6361 ttgcacctct gcatcgtcaa atgcagcaaa attcattttt gcagttttag cgcggttatt
     6421 cggttcgctg cgaaaacgat aaaaatcagc tagacccgag cctgcaatgg tgagggctac
     6481 cgcttggcag ccgaggcaac aaaatgcgtg tggctggttg ttgataggtg cggaataatc
     6541 cgggtgcgat tccaaaccac aatggtaaca aggtggaaat tgcaagaatt attgcacctc
     6601 tggggcgagt gtcagggttt gttgttggtt taaatccagc tcgcctacta agcgccattt
     6661 tttatcggct gcagcatcgg ccggggtgag ctgtagatac cagcggcctg cgagttgttt
     6721 gggtaaatcc acgcgataac ggctggcatt tatttttttt aattgcacgg ttaaatcgtc
     6781 ggtggcctta accgggtggg aaaaatacag cgtaagttgg tcgggaaaaa cgcttaaacc
     6841 tgttagctcc accagggctt ccccggacac agtatcaacc cgtacttgag caccaacgcc
     6901 cagggcggcg gccagtttat ccgcctcaaa gcggtggtta atggctttgc cttcggtgta
     6961 ataatcatcc accacacgct gatcagcacc gctcacggcg atataaacaa atatgagtga
     7021 ggtaagtatg gtgaggatta aagggcctag caccatccaa aaccagggtt ctcgccagga
     7081 gcttttttta ccggctaccg acgcttccac tgcggctggg ttaatcatgg gagggcccca
     7141 taaaggttga atcttccgag gcacttaaac ccgtttccac gctggtggcg ctgatggtaa
     7201 tggtattttc ctcgcctttt agctcggctt taggcactgc cacccgcacc ggcagcgaat
     7261 aaacttcccc ggatttaaca tgcacctggc gggcgttaag cacgcggaac gagtacaggc
     7321 ctttaacatc gacgacaaag gtgtgttcct gctggtccat gttgttaatt tttacggtgt
     7381 agacgttttg aatgtcgtcg ccatcgaggc gaaataaatg ggccgagcgg tcgcgcacca
     7441 catcaacgct tagaggctca cggatggata agcgatagga aaacgccgct accataatca
     7501 gcatcgccac tgcatagccg atggttttgg ggcgaataat gcggtatttg ccggtggtta
     7561 attgagtttc tgaggtaaag cgcaccaggc cgcgctcgta ccccatttta tccataacag
     7621 aatcacaggc atcaacacac aggccgcagt tgatacattc ccattgggtg ccattgcgaa
     7681 tatcaatatt caccgggcac acctgtacac accaggagca gtcggtacag tcgcccagcc
     7741 ccttggcctt gtaatcttcg ttcggcttgc gcgggccgcg ctgctcgccg cgggcgctgt
     7801 cgtattgcac gtaaatggta tctttgtcgt acatggccgc ttggaagcgg gaataggggc
     7861 acatgtattt acacacctgc tcgcgcatcc aaccggcgtt gaggtaggtc atgccggagc
     7921 agagcagcac aaaaaatatc ggccagccgc ctaactcaat accgatgtgt ccgtctggcg
     7981 tgatatgggg cagaagccct ttgaccagtt ccggcgccgg tacaaaataa ccgacgaaag
     8041 taatgccggt aaaaaatgcc actaaaaacc agagcagatg tttgctgcct ttgcgcagaa
     8101 ttttttcccc gtcccagctc tgtttatcca gtttgatgcg tttgttgcga tccccttcgc
     8161 agaggtcctc aatcacaata aaaattaatg tccagatggt ttgcgggcag gtgaagccac
     8221 accatacacg gccaaaaacg gtgcttacgg taaataaggc gtaggcggcg ataataaaca
     8281 gccaggccag cagcataaga tcctgcggcc agaaggtaat cgataaaaag tggaattttt
     8341 ggtgggccat gtccagccat acagcggggc ggccatcaat ggaaatccag ggcagcaata
     8401 caaatagcgc aaccagcggg atgcctgagt agcggcgcag gcgctgataa aagccggata
     8461 ttttgcgggt gtaaatttta gcttcgggtt cgtataaatt gacgtaacga atttccggtt
     8521 tgtcgccctg cggtttgtct gtcactgctt caacctattg gggggaatag agaaggggct
     8581 gccgaggcag cccgtagacg cattatctat ttagccgcgt tgttggaaag atgatacacg
     8641 taggcagcta gcaggtggat cttctctggt aacagagtgt gttcctgggc gggcatctgg
     8701 ttgttgcggc cattgcggat ggtttggcga atattttccc gcgtgccctc atacaaccag
     8761 gttttatcgg tgaggttggg agcgccggtg gcgtattggc ctttggcgtc tgtgccgtgg
     8821 caggcaacac agttctcggc aaatacctta gcaccgagtt ccgcttgggc
//
LOCUS       DAOQGS010000107         8860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3729_length_8860_cov_5.234299, whole genome shotgun sequence.
ACCESSION   DAOQGS010000107 DAOQGS010000000
VERSION     DAOQGS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3729_length_8860_cov_5.234299"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1391)
                     /locus_tag="PKZ52_05705"
     CDS             complement(87..1391)
                     /locus_tag="PKZ52_05705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG59301.1"
                     /translation="MALLHPSSLAGNPRRYIIFTVFYNARAYYPVLAVLFLDLGLSLD
                     QFVLLNLIWALTIFFCEVPSGALADTWGRRNLLVAAAVLMIIEMLCLLIAPVQGGSLL
                     IALCVLNRLCSGLSEACASGADEALAYDSLPTENRAQAWDKILADAMRWRSAGFMLAM
                     LLGGFLYDPSGLNKLLPSDLAIGTEAAHRLPIYLVLGQGFICLWASLTMVEPARQAAP
                     VSLAALTRIIFASALWLWQQKLLRVLVLTGLVLDAVVRNFATLNSSYYRLIELPEWSF
                     GVIGAGLGLAGIAVPSLAKYLNSRLSPQANINLTAICVLILLAALIPAWPLWGILPAS
                     LLMLSMGFLGFTLSKAIHQAASSDNRATVLSVKGLVFNLGYGLMSLGFSALLAKAGSD
                     AQGQNLIMALQWQWPLIAVILAVLIMWGAWVLRHPPAKVNKV"
     gene            complement(1469..1831)
                     /locus_tag="PKZ52_05710"
     CDS             complement(1469..1831)
                     /locus_tag="PKZ52_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNG59302.1"
                     /translation="MLNAEFAHSYAKHWVEAWNSRDINSILSHYHDDFVMSSPKIATI
                     AGEPSGVLRGKSAVAAYWTKALSLIPQLNFVLADVFIGADSLILHYHSFSGPVTEVFF
                     FNEQGLVVKAAAHYLATA"
     gene            complement(2215..2787)
                     /locus_tag="PKZ52_05715"
     CDS             complement(2215..2787)
                     /locus_tag="PKZ52_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59303.1"
                     /translation="MKVSNRLFMAALCLVSCSARAETHQPFISIMAAPFAALDAGEPQ
                     EEYSQSGAASQQEHFSMNLHRGYGVRISAYNMYASITQAQTSSDSEIPNADFKVITLG
                     FLFDENDDESYPVGLYSRAGIGFGAGGFDYARTELNESNLVSEVFLEGGLTLQHTIQI
                     GIQGKLQSLFRVGDTKAVIGEVALVASLHL"
     gene            complement(2837..3667)
                     /locus_tag="PKZ52_05720"
     CDS             complement(2837..3667)
                     /locus_tag="PKZ52_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNG59304.1"
                     /translation="MHIHALNLYPVKSLAGISLPRALLTAKGLATPCHDGLLYDRQWM
                     LINAAGQMVTQRQLPELALVQPVIEDDQLFLTAPGLPPIALTGLEVDAPCEPSQVWKT
                     PVQVQALAALTDWFALVVPGASLRLVQLAAPRPMLTERFGSSSETHFADAAPYLVANT
                     ASLAALNQALISEGETPVDERRFRANIWLDGITAFTEHQVPVLSLPQGQIRMVDHCQR
                     CSVITVDPERGVFCPNAKPFKTLARLNAMPNQPKAPAFGVNAVFEQTENTWVAVGEWC
                     "
     gene            complement(3695..3997)
                     /locus_tag="PKZ52_05725"
     CDS             complement(3695..3997)
                     /locus_tag="PKZ52_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009021617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HNG59305.1"
                     /translation="MSEIEWQAAAFRRLVAHLQAHPEVQNIDLMNLAGFCRNCLSKWY
                     QEAAAADGSALTYEQTRELIYGMPYGEYKTRYQTPTTPEQQAAFEQKHQAQSSQQQ"
     gene            4556..5509
                     /locus_tag="PKZ52_05730"
     CDS             4556..5509
                     /locus_tag="PKZ52_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNG59306.1"
                     /translation="MNTVFTQVLRTCALFVICTLSIFITAVRAETFTQNSASGIRDGM
                     YFSFYKTDGSAEFTLEEPGRYTLKWDQSSEAVYGGIGWQYGSPAVIEYSGIFGNSGFS
                     NQNVWLGFHGYIGSHSHEFYIIESWGSYNPGICSGGESFANYQSDGATYSLCRIKKFL
                     QITIDPQWYYQYYSVRTIRKNFGAIKGSISLENHINAWQKNGFDVEGPIRYSSLMTDA
                     YRSLGMSDITVVAGSSNFILSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGSSS
                     GKPVNGAVAGALDIWVVLLMAAGLSRRCRKTDKFHCYTLRH"
     gene            5511..5726
                     /locus_tag="PKZ52_05735"
     CDS             5511..5726
                     /locus_tag="PKZ52_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59307.1"
                     /translation="MLISSTTLTRPQQRAVHNANNGTALLQTADLSIEQSAPILQRLR
                     MLAIPSPNGLYTNSDRMSWTMCTYKSH"
     gene            5938..6504
                     /locus_tag="PKZ52_05740"
     CDS             5938..6504
                     /locus_tag="PKZ52_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59308.1"
                     /translation="MNITLTRKAYRQFLIAFRAADIANAATGAETQVDYDASCSFFFE
                     APNFKLVAYQRAGQARVNLTNYNHVPNIGVTSLGDFSAALQNGGGTSTTMNRALSLVV
                     IAISEAARSRVVEEAMYKIITGGSVNMDNYEFLFKAYNQPARYNGFLKVDSSYSTYWR
                     PLKKEDYDRFFSSAEYTGKDMKGSLALV"
     gene            complement(6623..7312)
                     /locus_tag="PKZ52_05745"
     CDS             complement(6623..7312)
                     /locus_tag="PKZ52_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNG59309.1"
                     /translation="MSSIGIEQIKAVAGYLGDLCAHVVFVGGATTALLVDSVASDGAR
                     QTEDIDFVIDISLPGDFQLFERKMRERGFEHDQSQGAPICRWIIPHLGTRLIVDAMPM
                     DEKILGFSNRWYHQVIATAWQLPLDSQFIINVVDSVYFLGTKFEAWLGRGKNDIYAHD
                     LEDIFYVLEHRTGIEQDIFAAESAIQKYLAVQAAALLSHPGLDNNLPGLVDSVSLVKA
                     RLKFIARLGSN"
     gene            complement(7309..7908)
                     /locus_tag="PKZ52_05750"
     CDS             complement(7309..7908)
                     /locus_tag="PKZ52_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59310.1"
                     /translation="MEILKPFDLVVALKVGLNERALRSVQTGVIEAPPLTNSMSDLAE
                     ALGRGKADVSRSIARLLSAGLLGERDPGEGDLLAQNRKYYSVQRQGLSDLLIYGVRYV
                     FAPRILGFGRGVATGWNCPWVRSAINPPDVPLVWPTPGGESRGQWLEPLYPGVPTAAE
                     RDQDLYALLALIEVLRIGKPRELKYAQEMVYERVMDLHQ"
     gene            complement(8061..>8860)
                     /locus_tag="PKZ52_05755"
     CDS             complement(8061..>8860)
                     /locus_tag="PKZ52_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNG59311.1"
                     /translation="GSSSALASAYGIAVTLTMVITTLLIIVVALLIWRLSPVWVGLVG
                     LLFLLLDGSLFASCAVKLMDGGYFPLALGLLLFCLMSTWRRGRSLVMQALGAEGIPLE
                     DFVQDLDVNSLPHANRIAVYAVADSRFVPQALLHNLKHNQVLHRRNLIVNVRFAQAPW
                     VPEQERTIIELLGKEFWRITFTYGYMEVPDMPRALAACQQYGLAVPLFETSYFLSRET
                     IVSVPGGGMARWRELLFAAMNRNASGVVEFFCLPDNAVVELGTRLQI"
BASE COUNT         2353 a         2359 c         2120 g         2028 t
ORIGIN      
        1 aatcctcatt tactgcgtgt aaactccggt tttttcgcca aatttgcctt gccagcgcgt
       61 cgccgcctac gtatttcaac aagctgttag actttattca ctttggctgg cggatgccgc
      121 agcacccaag cgccccacat gattaacacc gctaaaataa ctgcaattaa gggccactgc
      181 cactgcaggg ccattattaa attttggcct tgagcatcgc tacctgcttt agctaatagc
      241 gcggaaaacc caaggctcat cagcccataa cctaaattaa ataccaggcc cttcaccgat
      301 aacacagttg cgcggttgtc gctgctggcc gcttggtgaa tagctttgct tagggtaaag
      361 cctaaaaagc ccatactgag cattaatagg ctagcaggaa gaatgcccca caatggccag
      421 gcgggaataa gggcagcaag cagaattaat acacagattg ccgtaagatt aatattggcc
      481 tgagggctta accggctgtt taaatatttg gccagggacg gcacagcaat tcctgctaag
      541 cctaagcccg cgcctatgac gccaaaactc cattcaggta attcaatcaa gcggtaataa
      601 ctgctattaa gcgtggcaaa attgcgcacc acggcgtcga gtaccaggcc ggttaatact
      661 aaaacccgca ataatttttg ttgccacaac caaagggcgc tggcaaaaat aatccgcgtt
      721 aatgcggcca aactgacagg cgccgcctgg cgggcgggct ccaccatagt gaggctggcc
      781 cacaaacaaa taaaaccctg gccgagcact aaataaatgg gtaagcggtg ggccgcctct
      841 gtaccgatgg ctaaatcgct gggtagcagt ttatttaatc cgctgggatc gtataaaaag
      901 ccacctaata acatggccag cataaaaccg gcggaacgcc agcgcatggc atcggcgaga
      961 attttatccc aggcctgggc gcgattttca gttggcaggg aatcataagc cagggcttcg
     1021 tcagcaccgc tggcgcaggc ttccgataat ccagagcaca ggcggttgag cacacacaac
     1081 gcaattaaca ggctgccgcc ttgcacgggc gcaattaaca aacacagcat ttcaataatc
     1141 atcaatacgg cggcggccac taataaattg cgacgccccc aggtatcggc cagggcgccg
     1201 gagggtactt cacaaaaaaa tatggtcaac gcccagatta aattgagcag aacaaattga
     1261 tccaggctta aacccaagtc taaaaataac accgccaata cagggtaata ggcccgcgca
     1321 ttgtaaaaaa cggtaaaaat aatgtagcgc cttgggttgc cagcaaggga tgatggatgg
     1381 agtaaggcca tgaataattc gcgccgccga gtgagtgatt tgggataaca atgcgccaca
     1441 caggtgcggc ccagattggg tgcagctttt aggcggtggc caaataatgt gcagcagctt
     1501 taacaaccaa gccttgctcg ttaaagaaaa acacttctgt aactgggccg ctaaagctgt
     1561 gataatgcaa aatcaagcta tcggccccga taaaaacatc tgccaataca aaatttaatt
     1621 gcggaatcag cgatagcgct ttagtccaat aggcggcaac cgcagattta ccccgcagca
     1681 caccggatgg ctcacccgct atggtggcaa ttttcggtga cgacataaca aaatcgtcgt
     1741 gataatgact gaggatgcta ttgatatcgc gtgagttcca ggcctcaacc cagtgtttgg
     1801 catagctgtg tgcaaattct gcattcaaca taaaatattc ctaattctaa aaattgcggc
     1861 ctatacctgt tgtacggaag caaagaatac acatcgtttc agaccgtcaa aaagagggat
     1921 ctttttgacg agcctacacg gatgtattta cggcgtgtct gaaatgatgt gtattctttg
     1981 ctggtaccta ggtagtaaat ttagtagtct atttttgtag tgaacagctt gttgaacttt
     2041 gttggctgcg acgctctggc aagacaaatt tggcggaaaa ccggacgact gcaggaatac
     2101 ccatgcagga gcaattgctg ggtttacatg cattaaatga agatttttgg ccatatttaa
     2161 cgccgcccac ccacgcaagg tactatttca acgcgctgtt aaaagagctt tgagctatag
     2221 atgtaggctt gccaccaggg ctacttcacc aatcactgcc ttggtatcgc ccacccgaaa
     2281 taaactctgt agttttccct gtattccaat ttgaatcgtg tgctgtaggg ttagcccgcc
     2341 ttcgaggaat acttcagaga ccagattact ctcattaagc tcagtacggg cataatcaaa
     2401 acccccagca ccaaacccta tacccgcgcg gctatatagg cctaccggat aggattcatc
     2461 gtcattttca tcaaataaaa aaccgagtgt aatcacctta aaatctgcat tgggaatttc
     2521 actgtcggac gaggtctgcg cctgggtgat gctcgcatac atgttatagg cgcttattcg
     2581 cacaccataa ccccggtgca aattcatact aaagtgttcc tgctgtgatg ccgctcctga
     2641 ttgcgaatac tcttcttgcg gctcacctgc gtctaaggct gcaaaaggcg ccgccataat
     2701 gctgataaat ggttgatgag tttcagcccg agcactgcaa ctcaccaggc ataaagcagc
     2761 cataaataaa cgattactca ctttcactgt gtctcctttt gatatcgcgt ttgtacattg
     2821 cgcgcagaat tgtttattaa caccattcac ccaccgccac ccaggtattt tcagtttgct
     2881 caaacaccgc attaacccca aatgccggag ctttgggttg attgggcatt gcatttaaac
     2941 gggcgagggt tttaaagggt ttggcgttgg ggcagaacac gccgcgctct gggtctacgg
     3001 tgattacgct gcagcgttgg cagtgatcca ccatacggat ttggccctgg ggcaaactca
     3061 aaaccggcac ttgatgttcg gtaaaagcag ttatcccatc aagccaaata ttggcgcgaa
     3121 agcgccgctc atccacaggg gtttcacctt cactgatgag cgcctgattt agcgcggcta
     3181 aggatgcggt attggccacc agatagggcg ctgcatcggc aaagtgggtc tcactggaag
     3241 agccaaagcg ctcggtgagc atgggacgag gggccgcaag ctgcaccaag cgcaggctcg
     3301 cccccggcac tactaacgcg aaccaatcgg taagcgcagc tagtgcctgt acctgtaccg
     3361 gggttttcca tacctggctt ggctcgcagg gcgcatccac ctccaacccg gtgagtgcta
     3421 tagggggcag gcccggtgcc gtcaaaaaca actgatcgtc ttcgatgacc ggctgcacca
     3481 aggcaagttc gggcaactgc cgctgggtca ccatctgccc ggccgcattg attaacatcc
     3541 attggcggtc gtacagcaag ccatcgtggc aaggcgtggc aaggcctttg gcggttaaca
     3601 gcgctcgagg caggcttatg cccgccaggg atttgactgg atacaggttg agggcgtgga
     3661 tgtgcacgga gtacctttcg gcggggaatc cgccttactg ttgctggctg gattgggcct
     3721 ggtgtttttg ctcaaacgcc gcctgttgct caggcgttgt gggtgtttga tagcgggttt
     3781 tatattcgcc gtagggcatg ccgtaaatca actcgcgggt ctgctcatag gtcagggcgc
     3841 tgccatcggc tgctgcggcc tcttggtacc atttggacag gcaattgcgg cagaagcccg
     3901 ccaggttcat aagatcaatg ttttgtactt ctgggtgggc ttgcaaatgt gccactaggc
     3961 ggcgaaaggc cgccgcttgc cattcaattt ccgacataaa ttgttccgca aaaatcgcta
     4021 ttgaaggctt atcctagcgc ttaggcaagc agattgaaaa atttctcagc aacccgcgtt
     4081 ctgaacatct taagcccacc ctgaattaat cccggctcct aaccacaccc tctggtttaa
     4141 acagctgcgc aggtgtgaaa cacgccaaac ccgccccctg caaaaccaaa ccacttcaca
     4201 cattagcgcg atgccactgc cagggcgcaa tcgattaggg taggctttaa attgtattac
     4261 atttttgcat tcatcccgat gacaaaggtc tacgggggtt ttagcaaaca ggcgaagaaa
     4321 aagagttgct ggcggctggt taaaagcacc ccggcatacc gccgcgcacg acatataact
     4381 catagaccgg gccgccaatc gcggcacgcc tggctggcgc aacataaaag ggcagattag
     4441 gcagtgtgcc gaatataaaa agccgggctt ttaagtaccg ggttttgcac acgcaaaata
     4501 aaaaaatggc acatttaatg tgtgtgcctt tcccacagcc atggagacct tctatatgaa
     4561 tacagttttc acccaagttc tgcgcacctg cgcgctcttt gttatctgca cgctgagtat
     4621 ttttattacc gccgttcggg cagaaacatt tacccaaaat tcggcctcag gcattcgcga
     4681 tggcatgtac ttttcattct acaaaacaga tggctcggca gaatttacgc ttgaagagcc
     4741 aggccgttac acattgaaat gggatcaaag ttcagaagca gtgtatggcg gcattggctg
     4801 gcaatacggc agcccagcgg tcattgaata ttcaggtatt ttcggaaaca gcggtttttc
     4861 taaccaaaat gtgtggctgg gttttcacgg ctatattgga agccacagcc atgaatttta
     4921 tattattgaa tcctggggaa gttataatcc gggcatctgc agtggcggcg agtcctttgc
     4981 caattatcaa agtgatggcg caacctacag cctatgccgc attaaaaaat tccttcaaat
     5041 cactattgac ccacagtggt attatcaata ctacagtgtg cgtacaatca ggaaaaattt
     5101 tggcgccatt aaaggttcaa tctctcttga gaaccatatc aatgcttggc agaaaaatgg
     5161 ttttgatgtt gaggggccca taagatattc aagcctaatg actgacgctt atcgaagcct
     5221 gggcatgagt gatattacag tagtcgcagg ctccagcaat tttattctga gttcatcttc
     5281 gagctcttca tccagttcaa gttcttcaag ctcatcctca tcaagctcta gttcctcatc
     5341 gtcaagctca agcagctctt cctcaagctc cggcagttca agcggcaaac ccgtcaacgg
     5401 agcagtcgct ggcgccttgg atatttgggt tgtgttatta atggcagcag gccttagccg
     5461 caggtgtcga aaaacagata aatttcattg ctacacacta cggcattagt ttgttaataa
     5521 gcagtacaac tctgacgagg ccacagcagc gggcggtgca caatgccaac aatggcactg
     5581 cactgctgca gacggccgat ctctccattg aacagtccgc tcccattctg cagcgcctgc
     5641 ggatgctggc gattccaagc cccaacggcc tatacaccaa ctccgaccgc atgagctgga
     5701 cgatgtgcac atataagagc cactgaaaat atccacctat atcaaaatcc ccaacggcgc
     5761 cccttaaaat ttcgatctaa attggtagca aaatccctaa ttaaagggca agcacacagg
     5821 cgccactctc aatgtttacc taagaatttt ggcccggcga aaatcctgtc tcagcaattg
     5881 acacttaatc attaacaccg taaaaggttt ctcccccacc ccacccgagg acctgccatg
     5941 aacattactt taacgcgcaa agcctatcgc caatttctca tcgcctttcg tgcagcggac
     6001 atagccaatg cagctaccgg cgccgaaacc caggtcgatt acgatgctag ctgcagtttt
     6061 ttcttcgaag cccccaattt caagctggtg gcctaccagc gtgccgggca ggcgcgcgtg
     6121 aatttaacca attacaacca tgtgccaaat ataggtgtta ccagcctcgg cgactttagc
     6181 gccgccctgc aaaatggcgg cggcacttcc accacaatga accgcgccct cagtttagtg
     6241 gtgattgcta tttccgaagc agcgcgctca agggtggtgg aagaggccat gtataaaatt
     6301 attaccggcg gctcggtgaa catggataac tatgaatttt tatttaaagc ctacaaccaa
     6361 ccggcccgtt ataacggctt tttaaaggtg gactccagtt attcaaccta ttggcggccg
     6421 cttaaaaaag aagattacga ccggtttttt tcttccgccg aatacaccgg caaggatatg
     6481 aagggcagtt tggctttggt gtaacacctc tgccatcaca caactctatg cagggatgca
     6541 gcaagcctct tttttaaaac atcctaagta gaccatcgaa acataggcgc acaaaatact
     6601 gcgcgcaaag gttgcaacaa ttctaattac ttccaagcct agctataaat ttcaatcgag
     6661 ctttaaccag ggacaccgaa tccaccaacc ccggcaaatt attatccaat cctggatggc
     6721 taagaagtgc ggcagcttgt acggcaagat atttttgtat agctgattcg gcagcaaaaa
     6781 tgtcctgctc aataccagtt ctatgctcaa gcacataaaa tatatcttct agatcgtggg
     6841 cgtatatatc gtttttccca cgcccaagcc aagcttcgaa ttttgttccc aaaaaataaa
     6901 cggaatctac tacattaata ataaattgcg aatctaaagg gagttgccag gctgtggcga
     6961 tgacctggtg ataccaacgg ttgctaaagc ctagaatttt ttcatccatc ggcatggcat
     7021 caacaattaa tcgagtgccc aaatgcggaa ttatccaacg gcaaataggt gcaccctggg
     7081 actgatcatg ctcaaaacca cgctcccgca ttttcctctc aaacagctgg aaatcgccgg
     7141 gcagactaat atcaatgaca aaatcaatat cttctgtctg gcgagcgccg tcggacgcta
     7201 ccgagtcaac caataaagct gttgtggcgc cacccacaaa taccacatgc gcgcacaaat
     7261 cccccaagta tccagccact gcttttattt gttcgatgcc gatagaactc attgatgcaa
     7321 atccattact cgttcgtaca ccatttcttg ggcgtattta agttcgcgag gcttgccaat
     7381 acgcaacact tcaattagag ccaacagcgc atacaaatcc tgatcgcgct cggcggcggt
     7441 gggtacgcca gggtatagcg gctccagcca ttggcctcgg ctttcccctc ctggggttgg
     7501 ccatactaag ggaacatccg gggggttaat ggctgagcgc acccaggggc agttccagcc
     7561 cgtggccact ccacgcccaa agcctaaaat acgcggcgca aacacataac gcacgccata
     7621 gatcagcagg tcactcaacc cttgccgctg cacggaatag tatttgcggt tttgcgccag
     7681 caaatccccc tccccaggat cccgttcccc caaaaggccc gcactgagca agcgtgcgat
     7741 agaacgcgat acatccgcct taccccggcc caaagcctca gcaaggtcgc tcatggagtt
     7801 tgtcagcggc ggcgcctcaa ttacacctgt ctgcaccgag cgcaaggcgc gttcattcaa
     7861 acccactttg agcgcaacaa ccagatcaaa cggtttcagg atttccacag tatcgtttca
     7921 cgtttcaaaa catgaatcga ttttgatcaa cacaaatgca ttttgtcaac cacttattag
     7981 acacaagcag gcacacccca agctgccaaa cacagcccgt aaaccaaaac ccggctgcgg
     8041 gcatacctgt aggccgcgtg ttatatctgc agccgcgtcc ccaactccac caccgcgtta
     8101 tccggcagac aaaagaactc caccacgcca ctggcattgc ggttcatggc ggcgaataag
     8161 agttcccgcc agcgggccat gccaccgccg gggacggaga ctatggtttc ccggcttaaa
     8221 aaataactgg tttcaaacag cggcaccgcc aggccgtatt gctggcaggc ggcgagggcg
     8281 cggggcatgt cgggcacttc catatagccg taggtgaagg ttatgcgcca aaactccttg
     8341 cccagcaatt caatgatggt gcgctcctgc tcgggcaccc agggggcctg ggcaaagcgc
     8401 acgtttacaa tcaggttgcg ccggtgcagt acctggttgt gtttgaggtt gtgcagcagg
     8461 gcctggggta caaagcggct gtcagccacg gcgtacacgg cgatgcggtt ggcgtggggc
     8521 aggctattaa cgtccaaatc ctgcacaaaa tcctccaagg gaatgccttc agcgccgagg
     8581 gcctgcatca ccaggctgcg gccgcgccgc caggtactca tcaggcaaaa caacagtaag
     8641 cccagcgcca aggggaaata accgccgtcc atcagtttta ccgcgcagct ggcaaataag
     8701 ctgccgtcca agagcaaaaa caacagcccc actaagccca cccaaacagg cgacaggcgc
     8761 cagatcaaca gggccaccac aataatcaac agggtggtga tcaccatggt gagcgttaca
     8821 gctataccat aggccgaggc tagagcggag gagctgccga
//
LOCUS       DAOQGS010000108         8788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3767_length_8788_cov_4.478415, whole genome shotgun sequence.
ACCESSION   DAOQGS010000108 DAOQGS010000000
VERSION     DAOQGS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_8788_cov_4.478415"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKZ52_05760"
     CDS             complement(<1..502)
                     /locus_tag="PKZ52_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG59312.1"
                     /translation="MLNQPPGQVFPSDPQHTCAPSQRLHLILLGVQDVARSCQFYEAL
                     GWRKSPTGNDGFVKFNLGGYALCLISREALALDAGFNPTEGTGFAKQMAAEFSGIALI
                     YLAKSAEEVPQILAQATAAGGTLVKPATRTHWGVAGYFKDPDGHLFEVDYEDAWVFDG
                     EHHLVVD"
     gene            complement(547..1536)
                     /gene="pdxA"
                     /locus_tag="PKZ52_05765"
     CDS             complement(547..1536)
                     /gene="pdxA"
                     /locus_tag="PKZ52_05765"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNG59313.1"
                     /translation="MRIAITPGEPAGIGPELIVRYCQTPATTPCELVVFADGELLQQT
                     AAQLNVPLTLNAFNPAEQRLSSPGELSLVDIKCAAPVTPSQLNTANAQYVLTTLAAAQ
                     DACIKGECDALVTGPVQKSVINDAGIAFSGHTEFFAERSGCDQVVMMLATEGLRVALA
                     TTHLPLIDVSAAITPASLTRTIEILQQDLISQFGIAAPRILVCGLNPHAGENGHLGRE
                     EITTITPVLNDLRAKGYNLIGPLPADTLFTPKYLAHADAVLAMYHDQGLPVLKYKGFG
                     QAVNITLGLPFIRTSVDHGTALDLAGLGKADLGSLRVAINYAQNMIQARNRTV"
     gene            complement(1791..2126)
                     /locus_tag="PKZ52_05770"
     CDS             complement(1791..2126)
                     /locus_tag="PKZ52_05770"
                     /inference="COORDINATES: protein motif:HMM:NF022786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HNG59314.1"
                     /translation="MTDATQPAAIEDSEPLFFNRADAHIFLSNDQLDSMHKEGVAASM
                     LYASARFTAWLMASSFPDSKPMQEAKAGALEFFTERFRDMLNQNIDDYIANFDQYIKT
                     PPKPANDAQ"
     gene            complement(2144..3427)
                     /locus_tag="PKZ52_05775"
     CDS             complement(2144..3427)
                     /locus_tag="PKZ52_05775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNG59315.1"
                     /translation="MTLANTSLFLLCLALAGTAAAAPQKLDQVAAIVGKNVVTLSELN
                     SRIAEIKSRPDAEKIKLPPDDVLRKQVLDHLITESLQLASANRMGVKISPEQVNAAMT
                     NIAQKNRMTLEQFEAMLVSEGLSPAVMRQRLQREITVQQVQRGVVTQRIKVSDLEIEN
                     FLKSADAKFWISPEYHIGHFMIALPPSPSAAEVAEAEAKINAVYEELKNGADFAATAI
                     AKSNAQDALNGGDIGWRKSADLPSLFAELVADMPVGSLSKPARSPAGFHILKLLDKRS
                     DANQVITQSKVRHILVKTSAVVSDEEARAKLNKLRTSILGGADFAKLAKENSEDIGSM
                     LNGGDLGWSSPGMFVPEFEAAIAEAQVGAVSEPFKSQFGWHILQVTERRQEDMTKEVL
                     QRKAANILTSRRFEDELQLWLMELKEDAFIDIKVQ"
     gene            complement(3437..5854)
                     /locus_tag="PKZ52_05780"
     CDS             complement(3437..5854)
                     /locus_tag="PKZ52_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNG59316.1"
                     /translation="MPKRPARHNGLPKAYRLLGAALAMAGGTLQAAEPLDWVPKERLT
                     PEQQKRMGPSCSGLYLDPLAQEPAPQDLDKAPICVDANGAHMSEGQTMRLNGDVVITQ
                     SSRRLKAGQMLYDKQGDEAALTGGVEIRQPGAVIRGSEARVSMSKNQATFTGGDFVLH
                     DTHLRGAAKTIEQTATGKVILHQGSLTSCEPGSQTWLLEGEQLQIDPQSAQGSGRDIT
                     VSIAGVPVFYTPYIIFPVGSERQSGLLFPTLGLSNGKLDYAQPWYWNIAPNLDATLTP
                     RYAGGHGAMLETELRYLHRRANNEVRLAYLPNDAAELTEQEQALIDQGTQEQEIHPFK
                     DNERWLVNWQQQGGFDLPWYSKVDYSQASDVDYLRDLSTASFTANSDTYLNQSFTLGY
                     NFAHWRLNLRAQSYQNLLRDLDDTYRQLPRVNLDGLYQLGDFGLQLQNEYVHFSHPNS
                     RRLDGSSIITGSRSRLDYQLNWQYAPAAGFIKPAIGSQFLAYQLDDTGLLPGVDTAPS
                     LASHYLSLDSGLVFERDAGQQLLEPRLYYLYRAHTDHSALYHLTADNQAINFDTTPLT
                     FGYEQLFRDHRYAGGDRLDDANHLALSLTGRLLDDGYREFLSASVGQLVYFDRRQVFL
                     GDTPADSLAETRSDFAGQIKAAVSETLSAQTDLLYNPSNQRLMRLTSGLNLRQDGNLY
                     NLGYRFVRQNEEAESQVEQTIDQLDLSALHSLNPQWTLMARAFYDINGHKPMDTFIGT
                     QYDDCCYSLRVLARRWVNSKLAGLAQADQRQYDQGVFFEVELKGLGSSGQRITSLLKD
                     SIIGFNP"
     gene            6079..7122
                     /locus_tag="PKZ52_05785"
     CDS             6079..7122
                     /locus_tag="PKZ52_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNG59317.1"
                     /translation="MQPTLIDALSAWTAAQLSLRGELFSGWQPLAGDAGFRRYFRLEK
                     LPYLAVYAPPESENSLLFCNIARFLRQQGLAVPEIIGADFAQGFLLVSDLGNELYAAH
                     LSEDRVDGLYGEAINTLLQLQVTQPPEGLLAHYDAASLRREMELFPQWFIGELLQYSL
                     NADEQQLLAQCFSYLEQAVAKQPKVVVHRDFHSRNLIYRHTQQGRLLPPGLIDFQDAL
                     IGPASYDLVSLLKDCYIDWPQSQVRSFALAYYQQAQQLGIWPRLRDGEFIQGFDLMGL
                     QRHIKVLGIFARLSLRDNKHGYLKDLPRVLAYTLDVLGHYPALANVHQWFYLRLMPRI
                     EQQSWYYPHKLAQ"
     gene            7126..7839
                     /locus_tag="PKZ52_05790"
     CDS             7126..7839
                     /locus_tag="PKZ52_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNG59318.1"
                     /translation="MPLTRRAMVFAAGEGRRMLPLTATTPKPLLPLGDSHLIGANLKR
                     LAAAGITEVIINTAYLGEQIQAALGDGHGWGLRIVYSPEPFPLETGGALWQALPLLGE
                     EPFVLLNADVLCDYPLAKLCRHELTGGAFGHLILVPNPEQHSQGDFALNTQGLVLPSG
                     SPRYTFSGISLLHPNLVRQFPNARQVFPLREALSWAMQQGRITGEVYEGHWLDVGTPA
                     RLAAAQVLWAELCGDKIIP"
     gene            7897..8217
                     /locus_tag="PKZ52_05795"
     CDS             7897..8217
                     /locus_tag="PKZ52_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59319.1"
                     /translation="MLNNKTLALGAAFAAGLSLSVSALAKDAQFGATPMQAGYQLAAK
                     DAEGKCGGEGKCGANAKKKEEKKEGEGKCGAEMKKNGEGKCGAEMKKDGEGKCGAEKK
                     DDKK"
     gene            8221..>8788
                     /locus_tag="PKZ52_05800"
     CDS             8221..>8788
                     /locus_tag="PKZ52_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNG59320.1"
                     /translation="MSALLAQIGGAGLGLRRPLMQDLARLKPGDPIDFFEIAPENWLH
                     LGGRLGQKLRPFSEAYPIICHGLSLSIGGIGPLNRQLLLDIKNFIATHNIRHYSEHLS
                     YTGDEAQLYELFPLPLTWEAVQHTAARIRMAQDILGERIAIENATYYTLPAQDMSEVE
                     FINAVVAEADCQLLLDVNNLYVNSQNHHY"
BASE COUNT         1779 a         2385 c         2611 g         2013 t
ORIGIN      
        1 gatctacaac caaatgatgc tcaccatcaa atacccaggc atcttcataa tccacttcaa
       61 ataaatgccc gtccgggtct ttaaaatagc ctgctacacc ccagtgggtg cgggtggcag
      121 gttttaccaa ggtgccgcca gcggctgtgg cttgggcgag aatttgcggt acttcctcgg
      181 cagacttggc taaataaatt agggctatgc cgctaaattc cgctgccatt tgcttagcaa
      241 agcctgttcc ttctgtaggg ttgaaacccg catccagagc cagcgcttcg cgagaaatta
      301 aacacagggc gtaaccgcct aaattaaatt ttacgaagcc gtcgttgccg gtgggggatt
      361 ttcgccagcc caacgcttca taaaattggc atgagcgggc cacgtcctgc acacctaata
      421 aaattaaatg cagcctttgg cttggcgcgc aggtgtgttg cggatcggag ggaaatacct
      481 ggcctggggg ttgatttaac atagggcctg cgcctgaatt ttttaaatgg ataagtgaag
      541 cgctgattaa acagtgcggt tgcgcgcctg aatcatattc tgcgcatagt taatcgccac
      601 ccttaaactg cctaaatctg ccttgccaag gcccgctaaa tccagcgccg tgccgtggtc
      661 tacgctggtg cgaataaacg gcaggcccag agtgatattc actgcctggc caaaaccctt
      721 gtatttcagc accggcaggc cttggtcgtg gtacatggcg agcaccgcat cggcgtgggc
      781 taggtatttc ggggtaaata aggtatccgc aggcaggggg ccgatcaggt tgtagccctt
      841 agcgcgtaaa tcgtttaaca ccggggtaat tgtggtgatt tcttcgcggc ctaaatggcc
      901 gttttccccg gcgtggggat ttaagccgca gactaaaatg cgcggcgcgg caatgccaaa
      961 ttgagaaatt aaatcctgct gcagaatttc gatggtgcgg gtaaggctgg cgggcgtaat
     1021 ggcggcgctc acatcaatga gcggtaaatg ggtggtggcc agagccaccc gcaggccttc
     1081 ggtggccagc atcatcacca cctggtcaca accgctgcgc tcggcaaaaa attcagtgtg
     1141 gccgctaaag gcgatgcccg catcgttaat cacacttttt tgcaccgggc cggtcaccag
     1201 ggcatcacat tcgcccttga tacaggcatc ttgggcggcg gcgagggtgg ttaacacata
     1261 ctgggcgttg gcggtattta actggctggg tgttaccggg gcggcacact taatatccac
     1321 caggcttaat tcaccgggac tgcttagcct ttgctcggcg gggttaaaag catttagtgt
     1381 cagcggcaca tttaactgcg ctgcggtttg ctgcagcagc tcgccgtcgg caaataccac
     1441 cagctcacag ggcgttgtag cgggggtttg gcagtagcgg acaatgagtt ccgggcctat
     1501 accggcgggc tcgccggggg tgatggcaat tcgcatggtg tatcactcgt gttaaacgcc
     1561 gaagacctgt gggcgctgca cccacctatt tagagcttaa aaataagggg agagcattgc
     1621 ggatacgttt tatcgctatg aattaaatct gcgccgtcgc taattcttct gtgttgcaaa
     1681 ccgcccccac acatttttgt tgccgcccaa gggcgggctt gaacacaggg tttaattcaa
     1741 aagctcgtag tattaaatcg aaccaggccc tttaggaaat ttaaaaccgc ttactgagcg
     1801 tcgttggcag gtttcggtgg tgttttaatg tattgatcaa aattggcaat atagtcgtcg
     1861 atattttggt tgagcatatc gcgaaagcgc tcggtaaaaa attctagggc gcccgcttta
     1921 gcctcttgca tgggtttgct gtcggggaaa ctgctggcca tcagccaggc ggtaaaacgc
     1981 gcggaggcat agagcatgga ggcggccacg ccctctttgt gcatgctgtc taattgatcg
     2041 ttggataaaa aaatatgtgc atcggcgcgg ttaaaaaata ggggttcgct atcttcgatg
     2101 gcggccggtt gggtggcgtc tgtcatgggg gaatctcgct gtgttattgg acttttatat
     2161 caataaaagc atcttctttt aattccataa gccacagctg taattcatct tcaaaccggc
     2221 ggctggtgag aatattggcg gctttgcgct gcagtacttc tttggtcata tcttcttggc
     2281 ggcgctcggt gacttgcaaa atatgccagc caaattggga tttaaagggc tcgctgacag
     2341 cgccgacttg ggcttctgca atcgccgctt caaattccgg cacaaacata cccggactgc
     2401 tccagcctaa atcgccgcca tttaacatgg agccaatgtc ttcggaattt tctttcgcca
     2461 atttggcaaa atcggcgccg cctaaaatac tcgtgcgcaa tttatttaat ttagcgcggg
     2521 cctcttcatc gctcaccacg gcggaggttt ttaccaatat gtgccgcact ttggattggg
     2581 tgatgacctg gttggcatcc gagcgtttat ccaataattt taaaatgtga aaaccggcgg
     2641 gggagcgggc gggtttggag aggctgccca ccggcatatc ggccaccagt tcggcaaata
     2701 ggctgggtaa atcagcggat ttacgccagc ctatatcccc gccgtttaag gcgtcctggg
     2761 cgttggattt ggcgatggcg gtggcggcaa agtcggcacc attttttaat tcttcataga
     2821 cggcattaat tttagcttcc gcctcggcca cttctgcggc agaaggtgaa ggcggcaggg
     2881 caatcataaa atggcctatg tggtattcag gtgaaatcca gaatttagcg tcggcggatt
     2941 ttaaaaaatt ttcaatttct aaatcggaga ctttaatgcg ctgggtgacc acgccgcgct
     3001 gcacctgttg tacggtaatt tcccgctgca aacgctggcg catcacggcg gggcttaggc
     3061 cttcgctcac cagcatggct tcaaattgtt ctaaggtcat gcggtttttc tgcgcgatat
     3121 tggtcatcgc ggcgttgacc tgctcagggg agattttcac gcccatgcgg ttggcggagg
     3181 ccaattgcag ggattcggta attaaatgat cgagcacctg tttgcgcagc acgtcatcgg
     3241 ggggcaattt aattttttcg gcatctgggc ggcttttaat ttccgcaatg cggctgttga
     3301 gctcgctgag ggttaccacg tttttaccca ctatggccgc cacctgatcg agtttttgcg
     3361 gggcagcggc ggctgtcccg gcaagggcta ggcataacag aaacaggctt gtgttggcta
     3421 aagtcataaa ttcttcttag gggttaaaac cgatgatgct gtctttgagc aggctggtaa
     3481 tgcgttggcc ggagctgcca aggcctttta gttcaacttc aaaaaatacg ccttggtcgt
     3541 attggcgctg gtctgcctgg gcgaggccgg cgagtttact attcacccag cgccgcgcca
     3601 acacccgcag gctataacag cagtcgtcgt attgggtgcc gataaaggta tccatgggtt
     3661 tgtggccgtt gatgtcgtag aaggcgcggg ccattagggt ccactggggg ttcaggctgt
     3721 gcagggcgga tagatccagc tggtcgatgg tttgttccac ctggctttca gcttcttcgt
     3781 tctggcgcac aaagcggtag cctaggttgt acaaattgcc gtcctggcgc aggtttaagc
     3841 cgctggtgag gcgcatcaag cgctggttgc tggggttgta gagcaggtcg gtctgggcgc
     3901 tgagggtttc gctcacggcg gctttaattt gcccggcaaa gtccgagcgg gtctcggcta
     3961 gggaatcggc gggggtatcg cccagaaata cctgcctgcg gtcgaagtac actagctggc
     4021 ccacactggc gcttaaaaat tcccggtagc cgtcgtccaa taggcggcca gtgaggctga
     4081 gggccaggtg gttggcgtcg tccaggcggt cgccgccggc gtagcggtgg tcgcgaaaga
     4141 gctgctcgta gccgaaggtt aggggcgtgg tatcaaaatt gatcgcctgg ttatccgccg
     4201 tgaggtggta cagggcgctg tggtcggtgt gggcgcggta gaggtaatac aggcgcggct
     4261 ccagcagttg ctggcccgcg tcgcgctcaa acaccaggcc cgaatccaag ctcaggtagt
     4321 ggctggccag gctcggggcc gtatccacgc cgggcaatag gccggtgtcg tccaactggt
     4381 aggcgagaaa ttggctgccg atggcgggtt tgataaagcc tgcggcgggg gcgtattgcc
     4441 agttgagttg atagtccagg cggctgcggc tgccggtaat gatgctgctg ccatcgagcc
     4501 tgcggctgtt ggggtggcta aagtgtacgt attcgttctg cagttgcagg ccaaaatccc
     4561 ccaattgata gaggccgtcc aggttgaccc gcggcagttg gcggtaggtg tcgtccaggt
     4621 cgcgcagcag gttttgatag ctctgggcgc gcaggtttaa gcgccagtgg gcaaagttat
     4681 agcccagggt aaaactctgg tttaagtagg tgtcgctgtt ggcggtgaag ctcgcggtgg
     4741 agaggtcccg caggtaatcc acatcgcttg cctggctgta gtccactttg ctgtaccagg
     4801 gcaggtcaaa gccgccctgt tgttgccagt tcaccagcca gcgctcgtta tctttaaagg
     4861 ggtgaatctc ctgctcctgg gtgccctgat cgatcagggc ctgttcctgc tcggtcagtt
     4921 cagcggcatc gttgggcaaa taggccaggc gcacttcgtt attggcgcgg cggtgcaggt
     4981 agcgcagctc ggtttccagc atagcgccgt ggccaccggc atagcggggc gtgagggtgg
     5041 cgtccaggtt gggggcaata ttccagtacc agggctgggc gtagtcgagt ttgccgttgc
     5101 tcaggcccag ggtggggaat aagaggccag actgccgctc agaacccacc gggaaaataa
     5161 tataaggcgt gtaaaacacc ggcaccccgg cgatgctcac cgtgatgtcg cggccgctgc
     5221 cctgggcgct ctgcgggtcg atctgcagtt gttcgccctc cagcagccag gtttggctgc
     5281 ccggctcaca actggtgagg ctgccctgat gcaggattac cttgccggtg gcggtttgtt
     5341 cgatggtttt ggcggcgccg cgcaggtgag tgtcgtgcaa tacaaaatcg ccgccggtaa
     5401 aggtggcttg gtttttactc atgctgacgc gggcttcact gccgcgaatc accgcgccgg
     5461 gctggcggat ttccaccccg ccggtgaggg ccgcctcatc gccctgtttg tcgtagagca
     5521 tttgcccggc cttaagcctg cggctgctct gggtaattac cacatcgccg tttaggcgca
     5581 tggtttggcc ctcgctcata tgggcgccgt tggcgtccac gcagatgggg gccttgtcca
     5641 ggtcttgggg ggcgggctct tgggcgaggg ggtctagata gaggccgctg caggatggcc
     5701 ccatgcgttt ttgttgctcc ggtgtcaggc gctctttggg cacccagtcc aggggctctg
     5761 ccgcctgcaa ggtgccccct gccatagcaa gtgccgcccc caaaaggcgg taagccttgg
     5821 gcaatccgtt gtggcgagct gggcgcttgg gcatactggc gttcccgcag tgtcaatgtg
     5881 ggcggcgatt gtaccctaga aatttaccct gcagcccagg ccttagctgc cttggggcac
     5941 agattgtgct tttgaggctg ttctgccccg cgtttcgcag gcccaaaaac gtggcgggct
     6001 tggccgacac ccgcaggcct taagcgttat ggtggcgcct ttgcgcctgc gcactcacct
     6061 tttgtaaggc cccaatttat gcagccgact ttaatcgacg ctttatccgc ctggactgcc
     6121 gcgcaattga gcctgcgggg ggaattgttc agcggctggc agcccctggc gggggacgcg
     6181 ggttttaggc gctacttccg cctggaaaaa ttgccctacc tggcggttta cgcgccgccg
     6241 gagagcgaaa acagcctttt gttttgcaac attgcgcgct ttttacgcca gcagggtttg
     6301 gcggtgccgg agattatcgg cgcggatttc gcgcagggtt ttttactggt cagcgacctg
     6361 gggaatgagc tgtatgcggc ccatttaagt gaagaccgag tggacggcct ctacggcgag
     6421 gcgattaaca ccctgttgca actgcaggtt acgcagcccc ccgaaggtct gctggcccac
     6481 tatgacgccg ccagcttgcg gagggagatg gagctcttcc cccagtggtt tatcggcgag
     6541 ctgctgcagt actccctcaa cgccgacgaa cagcaactgc tggcccagtg ttttagctac
     6601 ctggagcagg cggtggcgaa gcagccgaag gtggtggtgc accgggattt tcacagccgc
     6661 aacctgattt accgccacac ccaacagggc cgcctgctgc cgccggggct catcgacttt
     6721 caagacgccc ttataggccc ggccagctac gacctggtct ccctgttaaa agactgttac
     6781 atcgactggc cccaatccca ggtgcgcagt tttgcgctgg cctattacca gcaggctcaa
     6841 cagctaggca tctggccgcg cctgcgggac ggagagttta tccagggctt tgatttgatg
     6901 ggcttgcagc ggcacatcaa agtgctgggc atcttcgccc gcctctccct gcgggataac
     6961 aaacacggct accttaaaga cctgccccgg gtgctggcct atacactgga tgtgctgggc
     7021 cactacccgg cgctggccaa tgtgcaccag tggttttacc tgcgcttaat gccgcgtatt
     7081 gagcagcaga gctggtatta cccccacaaa ctggcgcaat agcctatgcc gcttacccgc
     7141 cgcgccatgg tgtttgccgc cggggaaggg cggcgcatgt tgcccctcac cgccacgacc
     7201 cctaagcctt tattgcccct gggcgatagc catttaatcg gtgcaaacct taagcgcctg
     7261 gcggcggcgg ggatcactga ggttatcatc aataccgcct acctcggtga gcagatccag
     7321 gccgcattgg gtgatggcca tggttggggg ctgcgcatcg tttattcccc cgagcccttc
     7381 ccgctggaaa ccggtggcgc cctctggcag gccctgcccc tgttgggtga ggagcctttt
     7441 gtgctgctta acgccgatgt gctctgcgac taccccttag ccaagctgtg ccgccatgaa
     7501 ttaacaggcg gcgccttcgg ccatttgatc ctggtgccca accccgagca gcacagccag
     7561 ggggattttg ccctgaatac ccagggccta gtgctgccca gcggcagccc ccgctacacc
     7621 ttcagcggta tcagcctgct gcaccccaat ttagttcgcc agtttcccaa tgcccgccag
     7681 gtattccccc tgcgcgaggc cttaagctgg gccatgcagc aggggcgtat aacgggggag
     7741 gtctatgagg ggcattggct ggatgtggga acccccgcac gtttggccgc agcccaggtg
     7801 ctctgggcgg aactctgtgg cgataaaata atcccctgaa gcccgtgtaa atgcttatag
     7861 aatacggctc ccatccacac caataggatc attaccatgc taaacaacaa aaccctcgcc
     7921 ctcggtgccg cttttgccgc tggcctgtct ctgtctgtat ccgccctcgc caaagacgcc
     7981 caattcggcg ccacgcccat gcaagccggt tatcagctgg ccgccaaaga cgccgaaggc
     8041 aagtgcggcg gggaagggaa gtgcggcgcc aacgccaaga aaaaagaaga gaaaaaagaa
     8101 ggtgaaggca agtgtggcgc cgagatgaaa aaaaatggcg aaggcaagtg cggcgctgaa
     8161 atgaaaaaag acggcgaagg taagtgcggc gccgagaaaa aagacgataa aaaataagcc
     8221 gtgagcgctt tattagctca aatcggcggt gcgggcctag gcctgcgccg ccccttaatg
     8281 caggacttgg cgcgccttaa acccggtgac cccatcgatt tttttgaaat tgccccggaa
     8341 aactggctgc acttaggcgg ccgcttgggc caaaaactgc gccccttcag cgaagcctat
     8401 cccatcatct gccacggcct ctccctctcc atcggcggca ttgggcccct taaccgtcag
     8461 ctgctgctgg atattaaaaa ctttatcgcc acccacaaca tccgccacta cagcgagcat
     8521 ttaagttata ccggcgatga ggcccagctg tacgaattat tcccactacc cctcacctgg
     8581 gaagcggtcc aacacaccgc cgcccgtatt cgcatggccc aggacatact cggtgagcgc
     8641 atcgccatcg aaaacgccac ctattacacc ctgcccgccc aggatatgag cgaggtggaa
     8701 tttattaatg cggtggtagc cgaggcggac tgccagctgc tactggacgt gaacaacctc
     8761 tacgtaaaca gccaaaacca ccactact
//
LOCUS       DAOQGS010000109         8712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3814_length_8712_cov_5.415617, whole genome shotgun sequence.
ACCESSION   DAOQGS010000109 DAOQGS010000000
VERSION     DAOQGS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3814_length_8712_cov_5.415617"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /gene="htpG"
                     /locus_tag="PKZ52_05805"
     CDS             <1..1189
                     /gene="htpG"
                     /locus_tag="PKZ52_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNG59321.1"
                     /translation="WTRSRSEVTDEEYTEFYKHISHDFNAPLTWSHNRVEGKLDYTSL
                     LYLPGKAPFDMFNRDAARGLKLYVQRTFIMDDAEQFLPLYLRFIKGVVDSNDLSLNVS
                     REILQKDPNIDSMKSALTKRVLDMLDKMASSEPENYQKFWDTFGQVFKEGPAEDFANR
                     EKIGKLLRFATTHTGAATQDQSLDQYIERMKEGQGKIYYVVADNHNTAKNSPHIEVFR
                     KKGIEVLLLSDRIDDWLVGHLREYKEHHFQDVTKGELDLGAVETEEEKQQQEQAEKQF
                     ESLTERMKKVLDEQVTAVRITHRLTDSPACLVVGEDEIGSQMRRLLESAGQKVPTSKP
                     TIELNPTHPLVVKLDQEADEDRFADLAKVLFDQASLAEGGHLVDPAAYVNRLNKLLLE
                     LVK"
     gene            1542..1886
                     /locus_tag="PKZ52_05810"
     CDS             1542..1886
                     /locus_tag="PKZ52_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59322.1"
                     /translation="MFKINLGLSILTLCFISQAKATETGLIKTLTMSGINDPNHKNVV
                     QIDFGAEIMNTPGCNKQFAAIRSGPENNHLISLALAASMAGKPITIELNSSDIYFSSG
                     NRCTISRLAVSN"
     gene            2147..2473
                     /locus_tag="PKZ52_05815"
     CDS             2147..2473
                     /locus_tag="PKZ52_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59323.1"
                     /translation="MKHLIAATLFFGAFSAHASIVGATLNGAKVNAVRVESGWGFISF
                     DKPASLAPCSADRVYLNLDNEAHRVAYSTALAAFMSGKTVSVRADDVPSKVFGACAMY
                     DIYVSQ"
     gene            complement(2613..2873)
                     /locus_tag="PKZ52_05820"
     CDS             complement(2613..2873)
                     /locus_tag="PKZ52_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNG59324.1"
                     /translation="MIKKSITVTDQQDSSIKAQIERGHCGNESEVVRELIRERQLREQ
                     ETSREIEIIRAKLIQAEERGFVEQSPEEIFKDIKKGLGRNAL"
     gene            3314..3499
                     /locus_tag="PKZ52_05825"
     CDS             3314..3499
                     /locus_tag="PKZ52_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59325.1"
                     /translation="MHSIKQSAHELIDHLPEQASWDDVMHAVYVRQKLERSLQTAREG
                     QITSHEDAKKRFLNRAD"
     gene            3489..3803
                     /locus_tag="PKZ52_05830"
     CDS             3489..3803
                     /locus_tag="PKZ52_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNG59326.1"
                     /translation="MQIEWSNSALNDLEDIHHYIHKDSPFYANQFIEKVFDRVDRLAE
                     FPKSGRKIPEYDHPDWREVMVGDYRIMYFLDNKSLLILAVMHGSRDLNNPHNQPWSDD
                     RE"
     gene            3986..4651
                     /locus_tag="PKZ52_05835"
     CDS             3986..4651
                     /locus_tag="PKZ52_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59327.1"
                     /translation="MNLKNLSTRIQQLLDQSTAVLATKKKEYGGDSVDSGKFVGLRAS
                     ALSFIAMVYGNKHSHYTEFDRWVDDSYEYKAQQGHAILLSIQKEIEGGWIFSVKRLVS
                     AEIFKDFLEMAEHLLIQKYKDPAAVMIGSVLEENLRQLCITNNIETDFEKDGIFIPKK
                     ADRLNADLANAMIYSKLDQKAVTMWLDLRNKAAHGKYEEYSHVQVELMLQGVTDFLAR
                     AGQ"
     gene            4894..5166
                     /locus_tag="PKZ52_05840"
     CDS             4894..5166
                     /locus_tag="PKZ52_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59328.1"
                     /translation="MKNVYDYVFSQSWQTQELQLQQGVQYPIFTELGPFKPGEMAIFL
                     GFEDIDNHYGKFVFKKHDGEIVEFPGDYSGPTHTSFLQIKCALRKI"
     gene            complement(5424..6851)
                     /locus_tag="PKZ52_05845"
     CDS             complement(5424..6851)
                     /locus_tag="PKZ52_05845"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNG59329.1"
                     /translation="MQSSNFSCRILGLALFLTSAAVLVGCGGELTASSSSSSSSSSSS
                     SSSSSSNSSSSSSSSSSSSSSSSSSSGAIPDNAKLVYAVNAGGQAVTIAGVEYQTDRF
                     ATGGATNSTTAAITGAAQPALYQSERYGTFTYEIPVSNAAYSLKLHFAEITQTMKGAR
                     LFSASVEGQPVLQNFDLFAQVGRNVAYDVIVPPVSVVDGSLTIQMSTQIDAATLSGFA
                     IYSTNGGQFTGANNNAAASAGCGQTPTLKSGTVNLQGRTYTLRIPDNYDKSHPYRLIF
                     GLHGATGSSRDVAPSFFGLWSLAQGSTIFIAPDAVGGLWDSARDVVYLSDILNQVKTG
                     LCIDTAQVAVEGFSQGGAMAWSLVCARPGVYRAAVVHSGGGVARPASCTPIAFMSSLG
                     QSESGGAGQTSNSDFFAKQNGCTVASLPKAPRGGHACTDYTGCSTGHPTRWCDYDGGH
                     TPSPTDAGQSTSWMPQEVWNFLKRF"
     gene            complement(7378..>8712)
                     /locus_tag="PKZ52_05850"
     CDS             complement(7378..>8712)
                     /locus_tag="PKZ52_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNG59330.1"
                     /translation="SLSASIDNATISGFAIYSKTGTLEEVAGPLGRGENPFVQTNYTA
                     DPAPVVYGDRLYVYTSHDEDVTVNNFYTMNDWRLYSTTDMVNWTDHGSPAGYRTFSWG
                     RDSAWAVQAIERNGKYYLYVPLNNNSGSRIGVAVADNPVGPYKDPLGKHLAQGPTSNI
                     DPTVFIDDDGQAYMYWGNGTLQYVKLNQDMISYSGSIQTASLSGFVEGPWAYKRGSTY
                     YMVYAASGNGSEKISYATASRATGPWTYKGDIMDAGRTYTNHPGVVDYKGHSYFFYHD
                     SSLPGGNNYKRSVRVQEFKYGSDGSIPKLSMTGEGPEGLASLNPFMQVEAETIAFSSG
                     VKTENCTDTGGGQNVTQISVGDYIKVKNVNFGSGVASFAARVSSSTSNAKIELRLDSK
                     TGTLVGTCDVSGRSSWTTVTCPASGASGTHDLFLVFAGSGDNLFKFNWWKFE"
BASE COUNT         2274 a         2118 c         2179 g         2141 t
ORIGIN      
        1 gtggacgcgc tcgcgcagcg aagtcaccga cgaggaatac accgagtttt acaaacacat
       61 cagccacgat tttaatgcgc cgctcacctg gagccacaac cgcgtagaag gcaaactcga
      121 ttacaccagt ttgttatacc tgcccggcaa agcgccgttt gacatgttta accgcgacgc
      181 cgcacggggt ttaaaactct acgtacagcg cacctttatt atggatgacg ccgaacaatt
      241 cctgcccctg tatttgcgtt ttattaaggg cgtggtggat tccaacgatt tatccttaaa
      301 cgtctcgcgg gaaattttgc aaaaagaccc gaatatcgac agcatgaaat ccgccctcac
      361 caagcgggtg ttggatatgc tggataaaat ggccagcagc gagccagaaa attaccaaaa
      421 attctgggac accttcggcc aagtatttaa agaaggcccc gccgaagatt ttgccaaccg
      481 ggaaaaaatc ggcaaattat tgcgctttgc caccacccac acaggcgccg ccacccagga
      541 tcaatccctg gatcaataca tcgagcgcat gaaagaaggc cagggaaaaa tttattacgt
      601 ggtagccgac aaccacaaca ccgccaaaaa cagcccgcat atagaagtct tccgcaaaaa
      661 aggcatagaa gtattattac tctccgatcg catcgatgat tggttggtgg gccatttgcg
      721 cgaatacaaa gaacaccact tccaagatgt caccaaaggt gaattagacc taggcgcggt
      781 ggaaaccgaa gaggaaaaac aacagcagga gcaggcggaa aaacaatttg aaagcctcac
      841 cgagcgcatg aaaaaagtgc tggatgaaca agtcaccgcc gtgcgcatca cccaccgcct
      901 aaccgactct ccagcgtgtt tggttgtcgg cgaagacgaa atcggcagcc aaatgcgccg
      961 cctgctggaa tccgccggcc aaaaagtccc caccagcaaa cccaccatag agctaaaccc
     1021 cacccacccc ttggtggtga aattagacca agaggcggac gaagatcgct ttgcggattt
     1081 ggctaaggtg ttgtttgatc aagcgagcct ggctgaaggc ggccatttag tggacccagc
     1141 ggcttatgta aatcgcttga ataaattgtt gttggaattg gtgaagtagt ttttctcgca
     1201 gtgagagccc ggcttaggcc gggttttttt ggtctatttt cttcggtgtc ttgaactact
     1261 tttatacaaa acaccggtaa agacggttat tgcaaaacta ctggccggat cgtttaaagc
     1321 catttttaaa gcagttgggt aaggcaatcg ggttgagttg agatatgggt cgtagcgagc
     1381 gttttgtcgg tcgggcgtgc atgtatatat acctgcaggc ttgacgagga ggggcttagg
     1441 cggtactttc ggctcgctca tccatggatg atagtggtcc cgttaaaagt tggcggcatt
     1501 actaacattt aaatgttagg tattaaacta gaggtatgga tgtgtttaaa ataaatttag
     1561 gtttgtctat tttgacactg tgttttattt cacaggctaa ggccactgag acagggttaa
     1621 taaaaacatt gaccatgtcg ggcattaatg atcccaacca taaaaatgtg gttcagattg
     1681 attttggtgc ggagataatg aatacgcctg gctgcaacaa acaatttgca gccatacgtt
     1741 caggccctga aaataatcac ctaattagtt tagctcttgc cgcatctatg gccggaaaac
     1801 cgatcacaat tgagctaaat agcagtgata tttatttttc cagcggcaat cgttgtacca
     1861 tttctcggct ggcagtctca aattagattt ggcgttttcg gcataacgca tataactgaa
     1921 atctgctagt tttgaagcac ttaagacata ttctggactg caaaatatat gtcccacaaa
     1981 tcaagttggc gagaatgtgc ctggaatgca aaggcaaaat taattagtag tgcttaggca
     2041 tatatggcag catcaagcag cgcgccacac cttaccatgc attgttgtca atcaccatgc
     2101 ttcgacaaaa ctctgcttta gcgttaaata aaaattggag atttatatga aacacctcat
     2161 tgcggctact ttattttttg gagccttcag tgcgcatgca tcaattgttg gtgcaacctt
     2221 aaatggggct aaggtgaatg ccgttcgggt agaatccggg tggggattca tttcctttga
     2281 taaaccagcc tcactagcac catgctcagc agaccgtgtc tatctaaatt tagacaacga
     2341 ggcgcatcgc gtggcatata gcacagcact tgctgctttc atgtctggaa aaactgtatc
     2401 tgtccgagca gacgatgttc cttccaaagt tttcggcgca tgcgctatgt atgacattta
     2461 cgtgagtcag taactgcgct aatggatcaa ggacggatca gatcaagggg gctagttatc
     2521 ggcgtccctg cccctttgat ccggcaatag cctccaaacg tttcattgca cgcgaaaaaa
     2581 cgcgctaatc ctttctcgct tcatggctga gcctatagcg cattgcggcc cagccccttt
     2641 ttaatatctt taaaaatctc ctccgggctt tgctccacaa agccccgttc ctcggcttgg
     2701 atcaacttag cgcgaattat ttcaatctcg cggctggttt cctgttcgcg caactgccgt
     2761 tcgcggataa gctcccgcac cacttcgctc tcgttgccac aatggccccg ttcaatctgc
     2821 gccttgatac tactgtcctg ctggtctgta actgtgatgc tcttttttat catctcgata
     2881 ccttcacctc atcatccgag ctcccactac tgagtaggat agtttacgcc cgtgggtatt
     2941 aagaagcctc ggtggaccat gtttaggtaa aagctaatta agcgtgattg ttatcacatc
     3001 ggcacgaggg caatgcttgc tacccatttc gactcttccc tgggaagccc ctgtggagtg
     3061 gactgaaccg gcggttgtcc ggtgagcgtc ctgtgagcct atgcccaagc ccgttttaag
     3121 gtcttggggt gacaatcggg tggctgttgt atatgctggt ttaataggct ggctgagcaa
     3181 atttgcctct aatgagcggc tctcggaaaa cgatcaacac gctttaatta ttgtgatggt
     3241 tgacccgatt aagtgcgcaa ataagtcgcg cagtgacagc taaattttaa cgccccattt
     3301 caacgaggtt agtatgcaca gcattaaaca atctgcccat gaactgattg atcacctccc
     3361 cgaacaagcc agctgggatg atgttatgca cgctgtctat gtgcgccaga aattagaacg
     3421 ctctttacaa accgcccgtg aaggccaaat aacgtcccat gaagacgcaa aaaaaaggtt
     3481 tttaaaccgt gcagattgag tggtcaaatt ctgcattaaa tgacctggaa gatattcacc
     3541 actacattca caaagactca cctttttacg ccaatcaatt tattgaaaaa gtgttcgacc
     3601 gcgttgatcg cttagcagaa ttccccaaaa gcggtcgtaa aattcccgag tatgatcacc
     3661 ctgattggcg cgaagtaatg gtgggtgatt atcgaattat gtattttttg gataacaaat
     3721 cactgttgat attggccgta atgcacggca gccgtgactt aaataatcca cacaatcaac
     3781 cttggagtga cgacagggaa tagctgttag taggtaaggt gggttcgcca agcaacccac
     3841 catcgatctc gctgcacaga attattcttt taaacggcga gtttgcagtc gccaaatggt
     3901 ggattgtcgc tttgctccga agtaatctta tgtgttgatc gccgcgcccg tgatgtaaat
     3961 aatctaaaag tcaaaaggga accacatgaa cctgaaaaat ttatcaacta gaatccagca
     4021 actattagat caatcaactg cagttttagc cactaagaaa aaggaatatg gcggtgactc
     4081 agttgattca ggaaagtttg taggattaag agcgtcagct ttgtctttta ttgcaatggt
     4141 ttatggaaac aaacactcac actacacaga atttgataga tgggttgatg atagctacga
     4201 gtataaggcc caacaagggc acgcaatatt attgtcgatt caaaaagaaa tagaaggagg
     4261 gtggattttt tccgttaaaa ggctcgtttc ggcagagatt ttcaaagatt ttttggaaat
     4321 ggcagagcat ttactaattc agaagtataa agatcctgct gctgtaatga tcggtagtgt
     4381 acttgaagaa aatctacgtc aattgtgcat tacaaataat atcgaaacgg actttgagaa
     4441 agacggcatc tttataccca aaaaggctga caggctgaat gctgacctgg caaatgctat
     4501 gatctactca aaactcgatc agaaggcggt taccatgtgg ttagacctta gaaataaggc
     4561 agcacacgga aaatatgagg aatacagcca tgttcaagta gaacttatgc tgcaaggagt
     4621 tacggatttt cttgcccgag ctggtcaata atcactgaga gatcaagggg tcagagacct
     4681 tgaaacgaat tatcaatgcc cctgacctcc ttgatccgag aaagcatcag cgctcaccaa
     4741 gccgccgcca gcgccattac ggcgtaaagc gatggcccaa tttgccaagc cacccagtgg
     4801 aacctaatac aataattagc cctaaagaca agtcaatcgg tgggttggat tcccatccaa
     4861 gagattctca catcacattg tggagcgcgg caaatgaaaa acgtttatga ctatgttttt
     4921 tcacaatctt ggcaaacaca agaactgcaa ctccaacaag gagtgcaata cccaatattc
     4981 actgagctag gcccctttaa acctggggaa atggcgatat ttttgggctt tgaagatatc
     5041 gataatcatt atggaaaatt tgtatttaaa aagcatgacg gtgaaattgt tgaatttccg
     5101 ggcgattata gcggaccaac tcatactagc tttttacaga taaaatgcgc actcagaaag
     5161 atttaaatag acatttatca atattaagag gcagagatct ttaatattca caacgctgca
     5221 attatttttc aacgtctctg atctctagat cctgcgtctg cgcgcaggat gacaggtggc
     5281 ttgcgcagga tgaaaaaata aacaaatttc aaagcggctt atcctgcacg cagtcgcagg
     5341 ttacaaatac tctcgccagt ggtgcgcgcc gcatcctgcg acccgcaccc taaacttaaa
     5401 aaaccagcaa aaattagcct agcttaaaac cgcttcaaaa aattccacac ttcttgcggc
     5461 atccaggagg tactttgacc tgcgtcggtg ggagacgggg tgtggccgcc gtcgtaatca
     5521 caccatctag ttgggtgccc ggttgaacag cctgtgtaat ccgtacaggc atggccaccc
     5581 ctgggtgcct taggcagcga ggcaactgtg cagccatttt gtttggcgaa gaaatcactg
     5641 ttagatgttt gcccagcgcc gccactttcc gattgcccaa gagacgacat aaacgcgatg
     5701 ggggtacaac tagccgggcg ggcaaccccg ccaccggagt gaaccaccgc cgcgcgataa
     5761 acacccgggc gggcacacac caagctccaa gccatggcgc ccccttggct aaagccctca
     5821 acagcaacct gggcggtatc gatacacagg ccggttttta cttgattgag aatatcgctt
     5881 aaataaacca cgtcccgtgc gctatcccac aagccgccga cggcatccgg cgcgataaaa
     5941 atggtgctgc cctgggcgag ggaccagagg ccaaagaaag agggagccac atccctgctg
     6001 ctgcctgttg caccgtgtaa gccaaaaatc agccggtacg gatgggattt atcgtagttg
     6061 tcggggatac gcagggtgta ggtgcgcccc tgtaaattaa cggtcccgct cttgagcgtt
     6121 ggcgtctgcc cgcagcctgc tgaagcggcg gcattattat tagcgccggt aaattggcca
     6181 ccattagtgc tgtagatggc aaagcctgag agggtggcgg cgtctatttg cgtgctcatc
     6241 tgaatcgtta atgatccatc caccaccgat acaggcggca caattacgtc ataagcaaca
     6301 ttgcgcccaa cctgtgcaaa caggtcaaaa ttctgcaata ccggctgccc ttcaacactc
     6361 gcgctgaaca ggcgggcgcc tttcatcgtc tgggttatct cagcaaaatg cagtttcagg
     6421 ctgtacgcag cattgctgac ggggatttca taggtaaatg taccgtagcg ttcgctctga
     6481 tataacgccg gttgcgcagc gccggtaatg gccgccgttg tggaattagt agcgccgccg
     6541 gtagcaaaac gatccgtctg gtattcaacg ccagcaatgg tcaccgcttg tccaccggcg
     6601 tttactgcat acaccagctt ggcattatca ggaatggccc cggagcttga gctggacgac
     6661 gatgacgagc tgctggaact gctcgatgaa gacgagctgt ttgaggagga actgctcgat
     6721 gaactagagc tgctcgatga agaggatgaa ctgctcgcgg ttagctcacc gccgcatcca
     6781 accagcacag ctgcagatgt taagaaaaga gccagcccta ggatacggca ggaaaaattg
     6841 gagctttgca tggtttgaac gccaaagggt taggttggga taataaaagg tgtgcaatgc
     6901 gcgaataatc ggcatacacc ccttcgcctt gaagaattgc gttaaccgtt atgtgcggtg
     6961 cgtgtgcctt tgcgggcagc gacagctcaa ctggccagag cgttgaacgc cgccaatgac
     7021 ttgaagattg aattttgccc cggctcatgt ctttacaatc gacggggcag cttatgcctt
     7081 ggtgctcagc cacttccctg tggtttttca catttgtcaa acaaaagctc aatgtggggc
     7141 gcctagaaca gctcacatta agtcaccagt agcgctgcgg ctggtcgccc gtgaacagag
     7201 gcggcagccg gggcaacttc ttagactgcg ctattacacg ctaagcgggc aaaagctcaa
     7261 ggcgaccggt tttaatccgc cagcaaaact gcggatacgc aagcagttct gctgcgtacc
     7321 cgcaagcttg cgccgaggcg ccacagctaa atctcagcaa ctatatttcg tggaaaatca
     7381 ttcaaacttc caccagttaa atttaaagag attatcgccg ctgccagcga acactaagaa
     7441 gaggtcgtgg gtgccgcttg ctccgctagc agggcaggtc accgtggtcc aggaggagcg
     7501 tccggagaca tcacaggtac ccaccaatgt gccagttttg ctgtccaggc gcagctcgat
     7561 tttcgcgtta ctggttgagg aggatacgcg tgctgcaaag gatgctacgc cgctgccaaa
     7621 attgacgttt ttaaccttga tgtagtcgcc aacgctgatc tgggtgacgt tttggccgcc
     7681 gcctgtatcg gtgcaattct cggttttaac gcccgaggag aaagcgatgg tttccgcctc
     7741 cacttgcatg aacgggttta aactggctaa tccttctggg ccttcgccgg tcatggatag
     7801 tttaggaatg ctgccgtcgc tgccgtattt gaattcctgc acgcgcactg agcgcttgta
     7861 gttattgccg ccgggcaggg agctgtcgtg ataaaagaag taggagtgcc ccttgtagtc
     7921 caccacgcct gggtggttgg tgtaggttct acccgcatcc ataatgtcgc ctttgtaggt
     7981 ccaaggcccc gttgctctgc tggcggtggc gtagctaatt ttctcgctgc cgttgccact
     8041 ggcggcgtac accatgtagt aggtggagcc gcgcttgtag gcccacggcc cctcaacgaa
     8101 accggacaaa ctggcagttt ggatgctgcc tgagtagctg atcatgtctt ggttcagctt
     8161 cacgtattgc agtgtgccgt tgccccagta catataggcc tggccgtcat cgtcgataaa
     8221 tactgtcggg tcgatgttgc tggtagggcc ctgcgccagg tgtttgccca atgggtcttt
     8281 gtaagggccg acagggttgt cggcaaccgc aacgccaatt ctggaaccgg agttattgtt
     8341 cagcggtacg tacaggtaat acttgccatt gcgttcgatg gcctgcaccg cccaggcact
     8401 gtcgcggccc caactgaagg tgcgatagcc cgctggggag ccgtggtcgg tccagttcac
     8461 catgtccgtt gtggaataca gccgccagtc attcatggtg tagaagttgt tcaccgtaac
     8521 gtcttcgtcg tgggaggtgt agacgtacag cctgtcgcca tacacaaccg gcgctgggtc
     8581 cgctgtgtag ttggtttgca caaaggggtt ttccccgcgc ccgagtgggc cggctacttc
     8641 ttcaagcgtt cctgtcttgg agtagatggc aaagccactg atggtggcgt tgtcgatcga
     8701 ggctgacagg ga
//
LOCUS       DAOQGS010000110         8647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3862_length_8647_cov_4.976024, whole genome shotgun sequence.
ACCESSION   DAOQGS010000110 DAOQGS010000000
VERSION     DAOQGS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_8647_cov_4.976024"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PKZ52_05855"
     CDS             <1..1166
                     /locus_tag="PKZ52_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNG59331.1"
                     /translation="QSLGLALVKGILALVVIGVVGRFALRYLFQKAMASHSVDVFMAV
                     MLLAILATSMITELAGLSLSLGAFLAGLLLAETEYRHQIESEIEPFKGLLLGLFFMGV
                     GMNLDVALAFAHGLWVLASVVGLVAIKALVASLLARCFGLNWVCAVRTGFLLAQAGEF
                     AFVIIGQATLSYGLIDQALGQFMVVVAGLSMAATPLVAHLGQRFAQQLAARAPQDYQQ
                     VAVDLAAPHIVIAGYGRVGRAVATVLQRQGIAYVAVDMHSEAVAAARRRGEPVILGDA
                     RKPELLKKVGIASAQALIITMDNPIAAQQTLLSIRRQWPDLPIFVRSRDAAHSDVLLT
                     AGASAVVPETLEVSLQLASVALQSSGLGREAARLCVEELRQNNLAQWQDIERS"
     gene            complement(1191..1667)
                     /locus_tag="PKZ52_05860"
     CDS             complement(1191..1667)
                     /locus_tag="PKZ52_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59332.1"
                     /translation="MKLHTNFARANGSKNTAQYLLARGFGLWALVAAPVYAALEDDVV
                     GRWKLVSHLVTFDGQTFDSHQALLQQRPCAADIVYETNRDKTYRLNAAASSCDEKYKK
                     MQEKLYAKTQWKLDGATLTTSATHFAVGQSYTVSVAGKQMVWVGKDGQGTLTYQRQ"
     gene            complement(1997..2254)
                     /locus_tag="PKZ52_05865"
                     /pseudo
     CDS             complement(1997..2254)
                     /locus_tag="PKZ52_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013965.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
     gene            complement(2311..2466)
                     /locus_tag="PKZ52_05870"
     CDS             complement(2311..2466)
                     /locus_tag="PKZ52_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HNG59333.1"
                     /translation="MNLSATLEQALTAQLKAKQAQLWLEQNKDAIAAYNQSVDEQGVF
                     SDGLRSF"
     gene            complement(3234..4019)
                     /locus_tag="PKZ52_05875"
     CDS             complement(3234..4019)
                     /locus_tag="PKZ52_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59334.1"
                     /translation="MYERIKNFLSNPIYNLLIGILIALIFYLLADKKQDISYSVSNEE
                     IIASPTSASPDLSFIWKGNRINKELKRFKVAVWNSGNTYIDRNMLSTTEKLRIKIEGS
                     DSEILELNISSTSRPSLHIRAELNHEEQAVTLSIDGDDGLEQGDGFVVSMIYTGSPKP
                     KATLEGRIKGINKEFTYTNWSEINNGIAKNKKWAIAIALFFLLNATTLLITSTQKLLN
                     KPKLKLFFYDTAGILIGIGLFYSSIALLAMLFTSVYYNLKWVT"
     gene            complement(4300..6735)
                     /locus_tag="PKZ52_05880"
     CDS             complement(4300..6735)
                     /locus_tag="PKZ52_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNG59335.1"
                     /translation="MKFGHFDDNAREYVITNPKTPYPWINYLGNEDFFGLVSNTAGGY
                     TFYKDAKFRRLTRYRYNNVPVDNGGKYYYINDGGDVWNPGWKPVKADLESYSCRHGLS
                     YTRITGKRNGVEAEALYFIPLGTWAEIQKIKLTNTTSGVKKLKLFSFAEWCLWNAEDD
                     MTNFQRNFSTGEVEIQDSVIYHKTEFKERRNHYAFFSVNAPIQGFDTDRETFMGLYNN
                     FDRPDAVFEGKPRNSEAHGWSPIASHYIEVELAPGESREFVFVLGYIEVPKDQKWESK
                     GVINKAPAKEVIARFDTVEKVNAELEKLKEFWGNLLSTYTVNSGDERLDRMVNIWNQY
                     QCMVTFNMSRSASFFESGIGRGMGFRDSNQDLIGFVHQAPERARQRIIDIASTQFEDG
                     SAYHQYQPLTKRGNNAIGGNFNDDPLWLILSTTDYIKETGDFSILDQMVPYDNDDSKA
                     TTHFEHVKRSFYHTVNNLGPHGLPLIGRADWNDCLNLNCFSEEPNESFQTTGNKVGRT
                     AESLMIAGLFVLYGKEFVKLCNQIGKTGEALEAQGHIDAMIQAVKDHGWDGEWYLRAY
                     DYYGKKIGSKENEEGKIFIESQGFCGMAGIGLEDGMVQKSMDSVKEWLDTPYGIVLQQ
                     PAFTKYYIEYGEISTYPAGYKENAGIFCHNNPWIMITETLIGNGDRAFEYYSKIAPAF
                     LEEISDLHRVEPYVYAQMIAGKDAYKPGEAKNSWLTGTAAWNFAAITQYILGVKPDYN
                     GLGINPCIPKGWKGFKVSRKYRGATYNIEVKNPNGVSKGIASITVDGQAVQGNVLPLL
                     AAGSTGNVVVTLG"
     gene            7110..7925
                     /locus_tag="PKZ52_05885"
     CDS             7110..7925
                     /locus_tag="PKZ52_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4380 domain-containing protein"
                     /protein_id="HNG59336.1"
                     /translation="MLRIQNGPLSLAVDAATGGRITEFSYQDKNSLFTGKPAWGSTFW
                     PSPQSSWGWPPPAVLDQNPYTVVKHSATELVLKSGICPQTHLQVQKRIYAHGHGFAVE
                     YTHTNISQGVLSLAPWEITRVGGGVTFFLGKSTPQVQSTLALSHDGACWWHEYKVEQQ
                     RGENQKAFASDSEGWLANVHNGLLLLKRFPPVPNEKEAPGEAEIEIYAHGDKAHPYIE
                     VEQQGAYQTLAAGQSSSWTVQWDLLPAPADLLKPLQPAKLAAWVQTTIHQLER"
     gene            7935..>8647
                     /locus_tag="PKZ52_05890"
     CDS             7935..>8647
                     /locus_tag="PKZ52_05890"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HNG59337.1"
                     /translation="MTAPSRVVLVTGAASGIGFAISEHLASEGHHVILSDLNITAAEQ
                     AVGQLQAKGYSVEAWALNVASQADIEKTQAKFARVDVLINNAGLQHVAKLEDFPVEKW
                     RLLNDVLLVGPAMLTRAFLPAMKANNFGRIINIGSIHAVVASAFKSAYVAAKHGMVGF
                     SKVVALETAEFDITINSICPSYVKTALVEQQISQQAAQHGISEDQVINTIMLEPMPKK
                     SFISFAELAGTVSYLISPVA"
BASE COUNT         2006 a         1983 c         2289 g         2369 t
ORIGIN      
        1 ggcagtccct gggtctggcg ctggttaaag gcattttggc cctcgtggta atcggcgtag
       61 tggggcgctt tgctctgcgg tatttatttc aaaaagccat ggctagccac agtgtggatg
      121 tgtttatggc cgtgatgtta ttggctattt tggccacctc catgattacg gaactggccg
      181 gcctgtcctt atcgctaggg gcatttctgg ctggattatt actggcggaa actgaatacc
      241 gccaccaaat tgaaagtgaa atcgaaccct ttaaaggcct gctgctgggc ctgtttttta
      301 tgggcgtggg catgaacctg gacgtggcgt tggcatttgc gcacgggctt tgggtattgg
      361 catccgttgt ggggttggtg gccattaaag ccttggtggc aagtctgctc gcccgttgtt
      421 ttggccttaa ttgggtctgc gcggtgagaa cgggtttttt attagctcag gccggtgagt
      481 tcgcctttgt gattatcggc caggcaaccc tcagctatgg tttgatagac caagccctcg
      541 gccagtttat ggtggtagtg gcgggccttt ccatggccgc aacgccctta gtcgcccacc
      601 tcggccagcg ttttgcccag cagctggccg cccgcgcccc ccaggattac cagcaggtgg
      661 ccgtggattt ggcggcgccc catattgtga tagccggata tggccgcgtg ggccgggcgg
      721 tggcaactgt gctgcaacgc caaggcattg cgtatgtggc ggtggatatg cacagcgagg
      781 cagtggccgc cgcacggcgg cgtggcgagc cggttattct cggtgacgcg cgcaaacctg
      841 agctgttaaa aaaagtcggc atcgcctccg cgcaggcctt aattatcacc atggataacc
      901 cgattgccgc tcaacaaacc ctactttcga ttcgccgcca atggccggat ttgcctattt
      961 ttgtacgctc ccgcgacgcg gcccattccg atgtactgct aaccgctggc gcctccgcgg
     1021 tagtgccgga gaccctagag gtgagtttgc aactggccag cgtcgcgctg caaagctccg
     1081 gcctgggccg cgaggctgcg cgcctgtgtg tggaagaatt acgccagaat aatctggcgc
     1141 agtggcagga tattgagcgt tcataaaata gtgttggtat tgtaaggaat ttattggcgc
     1201 tggtaggtta gtgtcccctg gccatcctta cccacccaca ccatttgttt ccccgcaacg
     1261 gaaaccgtat aagactgccc cactgcaaaa tgagttgctg aggttgtcag cgtagcgccg
     1321 tctaatttcc attgggtttt ggcgtagagt ttttcctgca tttttttata tttctcatcg
     1381 cagctactgg cggcggcatt tagccgatag gttttatccc tattagtttc atacacaatg
     1441 tcggcggcgc aggggcgttg ttgtaataac gcctggtggg aatcaaaagt ctgcccatca
     1501 aaagtgacaa ggtgtgacac caatttccaa cggcctacaa catcatcctc caatgcggcg
     1561 taaaccggcg ctgcaactaa agcccacagc ccaaagcctc gggctaataa atactgtgcg
     1621 gtgtttttag agccatttgc tcgggcgaaa tttgtgtgca atttcataat tcattcctcg
     1681 taatattgag caaataaaag tgttgaagcg ctgtgctaag cacaggtaaa tgtcgcctga
     1741 gggtagcttg gttgtggaga gggggcaatg tgaatattac ctaaaatggt aattgttgct
     1801 tgcagcgcct tggtgctgtg gttttggcga agtaattatg cgcatcggtg cgggttaata
     1861 atattcaggc cgtgtcagca ttgcttgatg tatagatgca tttaaataca aagcgtccct
     1921 tgctgcgtca ctcctcagag cggcgcacca aggtacgtac aaatttaagc caggttgcgc
     1981 attacgagtg acaggcctag atgcccgtaa gcaggaagtc caacgcatta attatttcgc
     2041 cttttgcgta ttcagctgat gcaatagctt taccgagttc tttgcgcgga atacccgcca
     2101 attgaggcgt aagcataaga taggctgcac tttcaatgtt taatgggtgt taatcgggta
     2161 aaaagtttat ctctattcgg ttcaagtttt taagtggtac caaaactgtg gtttgcaatg
     2221 aatccagtag gttcgcttga acgtccagta gaaatggaaa ctctttttca ctttacgcat
     2281 tcttattctg gtaaataatg aattgcacga ttaaaaactc ctaagcccat cgctaaatac
     2341 cccctgttca tcaaccgatt ggttataggc agcgatcgca tctttattct gctccagcca
     2401 aagttgcgcc tgcttcgcct tcaactgcgc tgtgagcgct tgttctaggg ttgcggaaag
     2461 attgatgtcc agcgccttgg ccttttttaa taagtcgctg ttagctcaag ttggtaggtt
     2521 ttttaggtgc attaggttcg taaagcggaa gcataattgt aatctcactg tgtatgcgta
     2581 taggttatgc gcactggtgt gctgtcaaaa gcgcggattg tgtagcaatg ggaggggcta
     2641 ggtggagggc ttgagtatct gtgcttgttc ggcgccagct aggctcggga ttgctgggtt
     2701 gattgggtgg gtggctatgt atcgcccttg cctaattcat tgccggtcat attaaagtcg
     2761 atcgccaaac tggtcgcggc aattctcggg cgatatcttg tagggtgtgc aagcttgctt
     2821 gcccaccatt tgcttaaatt tcgacgagtc gaaatacagg cgatgcttga agtagtttgg
     2881 tgggcaacaa gctgcccacc aaactactta cttaaccgaa gcgtaaaata agctgagtcg
     2941 gcagcgagta ttcgcaaagg gcgcgatggt atttgtgata ataaaaaatg gcaaacagcg
     3001 ttgcatagtt cctagctgtg caggtgataa tacgaaatga agtggttaaa aaatattcgt
     3061 aggttgggca gtaatgccca acagattgac ggttttattg cagaattgcc tgtaattggc
     3121 aggttttgtt tggcgtttag ggttaaaaat taatatgggt tggcggataa atagaatgtt
     3181 gggcattact gcccaaccta cgagctgccc aacgagctag gttgtgcgta cagctatgtt
     3241 acccatttta ggttgtagta gacagaggta aaaagcattg ctaaaagtgc tatagatgaa
     3301 taaaaaagtc cgatgccaat taaaatgcca gcggtgtcgt agaagaataa tttgagcttc
     3361 ggtttgttta ataatttttg tgttgatgta attaaaagag ttgttgcgtt tagaagaaaa
     3421 aataaagcaa tagcaattgc ccattttttg tttttggcga tcccattgtt tatttcactc
     3481 caatttgtgt aggtgaattc cttgttaata ccctttattc gtccctcaag tgtggctttt
     3541 ggctttggcg aacctgtgta aatcattgaa acaacaaagc catcgccttg ttctagtcca
     3601 tcgtccccat ctatagagag agtgactgct tgctcctcat ggttgagttc ggcgcggatg
     3661 tgaagtgacg gccgggaagt agaagaaata ttcagttcta aaatctccga atcactgcct
     3721 tcaattttta ttctaagttt ctcagttgta gagagcatat ttctgtcgat gtatgtgtta
     3781 ccgctattcc aaaccgctac tttgaatcgc ttgagttcct tgttgattcg atttcctttc
     3841 caaataaaag aaagatcggg tgaggctgag gtgggggatg caattatttc ttcatttgat
     3901 actgagtagc taatatcttg ctttttatca gcaagcaagt aaaaaattag ggcaatcagg
     3961 attccaatta ggaggttgta tatggggttg ctaagaaagt ttttaattct ctcgtacatg
     4021 tgtgtatcct gattttttcc tgattatagg tgatgcatta ttttcatggg tatatttcgg
     4081 taatataaat tggttggcaa tcgcaaccag cttacatgct tgttttaggg cttacgttcg
     4141 catatgtgca agtgatattt taaggcgaaa tcaaatccat tcttaggggc cgcgtgcaag
     4201 atgcctttct agagcctgta ttcgtgtttc cgacattaaa gcaaaaaccc ccacaaagcc
     4261 aagccctgca ggggttttta aaccaaccta aaaaaccaat taacccagag taactaccac
     4321 attaccggta gagcccgctg ccaataaagg caacacatta ccctgcaccg cttggccatc
     4381 tacagtaata gaagctatac ccttgcttac accattaggg tttttcactt cgatattgta
     4441 ggtggcgccg cggtatttac ggctgacttt aaagcctttc cagccttttg gaatacaggg
     4501 gttgataccc aggccgttgt agtctggctt cacacccaag atatattggg taatagctgc
     4561 gaagttccag gctgcagtac cagtaagcca agagtttttc gcttcgccgg gtttataggc
     4621 gtctttaccg gcgatcattt gcgcgtatac atagggctct acgcggtgca gatcgctgat
     4681 ctcttccaag aaagctgggg cgattttgct gtagtactcg aatgcacggt cgccgttgcc
     4741 gatgagggtt tcggtgatca tgatccaggg gttgttgtgg cagaagatac cggcgttttc
     4801 tttgtaaccg gcggggtagg tggagatttc gccgtattca atgtagtact tggtgaaggc
     4861 aggttgttgc agcacgatgc cgtaaggggt atccaaccac tcttttaccg agtccatgga
     4921 tttttgcacc atgccgtctt ccaaacctat accggccatg ccgcagaagc cttggctttc
     4981 gataaagatt ttgccttctt cgttttcttt gctgccgatt tttttgccgt agtagtcata
     5041 ggcgcgcagg taccactcgc catcccaacc gtggtctttt acggcttgaa tcatagcgtc
     5101 tatgtggcct tgggcctcca aggcttcacc ggttttgcca atttggttac acagcttcac
     5161 aaactctttg ccgtataaca caaacaagcc tgcaatcatc aaagattccg cagtgcggcc
     5221 aactttgttg ccagtggttt ggaaagactc gttgggctct tccgagaaac agttgaggtt
     5281 taaacagtcg ttccagtcgg cgcggccaat cagcggcagg ccgtgggggc cgaggttgtt
     5341 taccgtgtgg tagaaggagc gcttaacgtg ctcaaagtgg gtggtggctt tgctgtcgtc
     5401 gttgtcgtaa ggcaccattt gatcgaggat cgaaaagtcg ccagtttctt taatgtaatc
     5461 ggtggtggag aggatcaacc agagtgggtc gtcgttgaag ttaccgccga tggcattgtt
     5521 gccgcgcttg gtgagcggtt ggtactggtg gtaggcagag ccgtcttcaa actgggtgga
     5581 ggcgatgtcg atgatacgtt gacgggcgcg ctcgggagcc tggtgtacga agccgattaa
     5641 atcctggttg gagtcgcgga aacccatacc gcggccgatt cccgattcaa agaaggaggc
     5701 ggagcggctc atgttaaagg tgaccataca ctggtattgg ttccagatgt tgaccatacg
     5761 atccaagcgc tcatcaccgg agtttacggt gtaagtggat aacaaattgc cccagaattc
     5821 tttgagtttt tctaattcgg cgttaacttt ttccactgtg tcaaaacggg cgatgacttc
     5881 tttagcaggt gccttgttaa ttacaccttt gctctcccat ttttgatctt tgggcacttc
     5941 gatataaccc aatacaaata caaactcgcg gctttcacct ggggccaact ccacttcgat
     6001 gtagtgagag gcaattgggc tccaaccgtg ggcttctgaa ttgcgcggct tgccttcgaa
     6061 tacggcgtct gggcggtcga agttgttgta caagcccatg aaggtttcgc ggtcggtatc
     6121 aaaaccttga ataggggcat ttacagagaa aaacgcgtag tggttgcggc gctctttaaa
     6181 ttctgttttg tggtaaataa cggaatcttg aatttccact tcaccggtgg agaagttgcg
     6241 ctggaagttg gtcatgtcat cttccgcgtt ccacaaacac cattcagcaa acgagaacag
     6301 cttgagtttt ttcacgccgc tggtggtgtt ggtgagttta attttttgaa tttccgccca
     6361 ggttcccagg gggataaagt acagagcttc ggcttcaacg ccattgcgct tgccggtgat
     6421 gcgggtgtag ctcaaaccgt ggcggcagct gtagctttct aaatcagctt taaccggttt
     6481 ccagcctggg ttccacacat cgccgccgtc gttgatgtag tagtatttac cgccgttatc
     6541 cacggggacg ttgttgtagc ggtagcgagt taaacggcga aatttggcgt ctttgtaaaa
     6601 ggtgtaacca ccggcggtgt tggataccaa accgaagaag tcttcgttgc cgaggtagtt
     6661 gatccagggg tagggggttt tcgggttggt gattacgtat tcacgcgcgt tgtcatcaaa
     6721 gtgaccaaat ttcatgggtc ggctccgctt gggatgaagg ctaaaaaaag cggccagctg
     6781 tgaaaggccg gccgcccgga taaaaaagcg cgctagtgta actggcttat ggtcgctttg
     6841 cagccgcctg cttcgaaaat tcttaccggc aacctgagct gattcgccaa ttaattcacg
     6901 ccggtacata tggctggcaa tttaaaggca tttcggttga atacaggttg agattagcaa
     6961 cagcgcatgt ttgggcggtt gcggaaaaga ttacttgtaa tacttttcgc agccgttgat
     7021 acagtgtttt cagttcaacc accatctgtc gctgtgcagc aattgtgtat actggcagcc
     7081 ctcatttaat tagccttgag acgcgcacta tgctgcgaat acaaaatggc cccttaagtt
     7141 tggccgttga cgccgccacc ggcggccgca ttaccgaatt ttcctatcaa gacaaaaaca
     7201 gtttatttac cggcaagccc gcctggggct ccaccttctg gccgagtccc caatcctcct
     7261 ggggttggcc accaccggcg gtgttggatc aaaaccccta cacagtggtg aagcacagtg
     7321 ccacggagct ggtgcttaaa agtggcattt gcccgcaaac ccatctgcaa gtgcaaaaac
     7381 gcatttatgc ccatggccac ggcttcgcag tggaatatac ccacaccaat atcagccaag
     7441 gcgtattgag tttggcgccc tgggagatta cgcgggtagg cggcggcgtt acttttttcc
     7501 tcggcaaatc tactccgcag gtgcagtcca ccttggccct aagccacgac ggtgcctgtt
     7561 ggtggcatga atataaggtt gagcagcagc gcggcgagaa ccaaaaagcc ttcgccagtg
     7621 atagcgaagg ttggttggcg aatgtgcaca atgggttgct gttgcttaag cgctttccgc
     7681 cggtgcccaa cgaaaaggaa gcccccggcg aggcggaaat tgaaatctat gcccacggcg
     7741 ataaagccca tccctacatt gaggtggagc agcagggcgc ttatcaaacc ctggcggcag
     7801 gccaaagcag cagttggacg gtgcagtggg atttattgcc cgcgcctgcg gatctattaa
     7861 agcctttaca gcctgcgaaa cttgccgcct gggtacaaac caccatccac caactcgaac
     7921 ggtaggagca caccatgact gcaccatccc gcgttgtatt agtgaccggc gccgcctcag
     7981 gcattggctt tgccatcagc gaacacttag ccagtgaggg ccaccatgtc attctcagtg
     8041 acctgaatat aacagcggcc gagcaggccg tagggcagtt gcaggctaaa ggctatagcg
     8101 tggaagcctg ggcgctcaat gtcgcatccc aagcggatat tgaaaaaacc caagctaaat
     8161 ttgcccgtgt ggatgtgtta attaataacg cgggcttgca gcatgttgcc aagctggaag
     8221 atttcccggt ggaaaagtgg cggctgttaa acgacgtgtt attagttggg cccgccatgc
     8281 tcacccgcgc atttttaccc gccatgaagg ccaataattt cggccgtatt attaatatcg
     8341 gctccatcca tgcggtggtg gcttccgcat ttaaaagtgc ctatgtggca gctaaacatg
     8401 gcatggtggg gttttctaag gtggttgccc tggaaaccgc cgagtttgat attacgatca
     8461 attcaatttg cccttcttat gtcaaaaccg ctttagttga gcagcaaatt agccagcaag
     8521 cggctcagca cggaatttca gaagaccagg ttattaacac catcatgctc gaacccatgc
     8581 cgaaaaaatc gtttatcagt tttgctgaat tggcgggcac cgtgagttat ttaatctccc
     8641 cggtggc
//
LOCUS       DAOQGS010000111         8626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3873_length_8626_cov_4.439272, whole genome shotgun sequence.
ACCESSION   DAOQGS010000111 DAOQGS010000000
VERSION     DAOQGS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3873_length_8626_cov_4.439272"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2375
                     /locus_tag="PKZ52_05895"
     CDS             <1..2375
                     /locus_tag="PKZ52_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59338.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSGLPESAIHKDVIRSICIYCHKIK
                     GAAEDSRLVFTNGPQYWKIEADNLAQLTQLAKTIGNVAILEKVRGASLHGGGIQLTPD
                     AYGYSVLKTFLEEVTGAQPTAQAPTNTVLESTRATYRRASLLLTGKVPEAYPSLINDA
                     DLTTALDKLMDNAEFYEFVKRGANDRLLTRSINSTLSLDFYLANYYPMYAAILASSPR
                     NNGYAQAVSREIGESVLELIAYVVKNNRPYTEILTADYTMVGPNTAVIFESNLSPRAG
                     EFLPAKDQGQLVSATQNQLPSSNWDATSKAFTEPVGILNNLGYLQQYQTNATNRNRAR
                     ARWTLQHFLGIDIENSSSRNIDASVLIDVQGGIPTRFACKVCHDRIDPVAGTFQRYGP
                     RGMYLDGAFGIDTLDSSYKRTAFYKTGDTWYRSMLPPGFEGTVFDLNNLIQDRDPLRE
                     LAEHIVQDPKFGEGTVKFWWPALFGEDILPYQLTSANLALRQTDLNEFATQFSSDNYN
                     FKHLLRTLILSPYFRSIPTKDNRYKQSRRLLTPEELHDKTQSLAHYADSRLLNDWLII
                     YGGIDSYNVEQRQRQFSPMMYQVAKQHAADASCELVNNLRADTSNGALTQPKQISQGF
                     TLATSATQEVTTTNTDHQLILRNNNSAPAAFSILFISNNGTVSQSERITLSGNTAWNS
                     AVHNSAVTWRITNESTAPINLTASSISLNTQALRTYFSELAFAFWGETDESMINELLA
                     FYFAARQLQDYNTINTCNLGSANSDVINTELASWRMVFIRMMTDYHYLYE"
     gene            2368..>3406
                     /locus_tag="PKZ52_05900"
     CDS             2368..>3406
                     /locus_tag="PKZ52_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNG59339.1"
                     /translation="MNNPTRRTFLRTGTALSLQSWLNPCIAIEPTTEPLFVVQLFANG
                     GLDCASFCDPKANINGTINNWATKLDIKQAGPFAFAPIADNEGFFTRHQDKILVINGV
                     NAQTNVHSAGRLTSLTGHNKNGYPSLCALHAAQHNTQLPMPLLVGNSFETGGIIAATQ
                     INGGLIEILKRPTQTDKKYLNAAQQQLIDNALNQRAAQSSAKAFYRAAIQGKQDYFAQ
                     TVDLYQSLSSDELPTKNTVVKDMKFALAAFAAGTSIACDYSISGFDTHTDHDKNMLVP
                     LNTLTSAADAAWYFAEQLQIDRRLLVIISSEFGRTPFYNDVAGKDHWPFHSVIIMKKD
                     ATWSGRIVGQTD"
     assembly_gap    3407..3417
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3891..6014
                     /locus_tag="PKZ52_05905"
     CDS             3891..6014
                     /locus_tag="PKZ52_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNG59340.1"
                     /translation="MKQLLQSLRNGDTLITEVPAPSVSPLQVLIRTHISAISTGTEKM
                     LVDFGKAGLVSKIKQQPDKVRLVLDKIKTDGLFTTLDTVRAKLDEPIALGYCNVGTVI
                     AVGNQVTEFAVGDRVVSNGNHAEIVAVNKNLCAKVPPAVADEDAVFTVLGAIALQGVR
                     LIDARIGETYVVMGLGLIGLLTVQLLRAQGCQVFALDLAADKIAAAEALGAKGILIQA
                     HNDSVQTIKNLHPNGVDGVLLTLSTQASEPVAQAAHMCRKRGKIVLVGVSGLHLSRAD
                     FYEKELSFQVSCSYGPGRYDPNYEVQGQDYPIGFVRWTQQRNFTAILQLMADGILKAQ
                     HYVSHRFAIDKAEQAYELLGQSGVSAILLEYPQNTLAPAQIIHYPQHTSAIAPTSAVK
                     IGFIGSGNYARRHLMPAFAKTGAEFLAVSCRSGVSGVKAAADFKISLACTDNQVIFNH
                     ADCNLVVISTQHNTHGRLVQQAIAAGKHVFVEKPLCLTQAELDSISTAVHTSANSLQI
                     TVGFNRRFSKLSQHLKKLAASTGEPLVISYSINAGFIDPSHWSQDVNIGGGRLLGEVC
                     HFIDLCRFFAGSKIVSSNVQALHKNGHPADSWQILLRFANCSIATINYFSNGHRSLPK
                     ETIEVNTAGKSLKIDNFRRLSCVGFGAGKEIKLWKQDKGQTQCALEVVQSIKDGRPAP
                     IAFAELHEVMQTCLDLWNQVQKSAP"
     gene            6379..7818
                     /locus_tag="PKZ52_05910"
     CDS             6379..7818
                     /locus_tag="PKZ52_05910"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNG59341.1"
                     /translation="MSKLWRYNLHYFDYLLAPSANEAPANCLNNADKNSLIQSWLEQN
                     PYPAEDAWEPYPTSLRIINWIKYFQRLGADLIPQHWHHSLYQQGHYLTHHIEWHIDAN
                     HLLKNIIALVIWSHYFNDTYARHWQQRSIKLLKAICQEQFFADGSHYERSPMYHFILV
                     HHLLDAYNCLINNTGTAPLWLKELLNRALSYCCTIVKPDGRIPLVKDSAHGIAPGIDD
                     LKAYATLLDIWPQENNTRPVKGELTACGYFIVKSEQDYILFDVGNLSPSHQPGHAHCD
                     ALHMEIFWDGRDVFTDAGVFNYVASPERDYARSVRAHNTACINGREQQELWGAFRIAR
                     RGVMGDVTHDEHSCSAEFYPYFSRNGHTGHKRSIKIDSSDTIEISEQLLGTGCAQAEI
                     FWHLAPGLLPQRNHNSIEIRNEKQIVVAVIKLQHKQVISIVTQSDYFPEFGKRCSRSC
                     VTLQINDAQLPMTLNYQIIKGSAAANICY"
     gene            8087..>8626
                     /locus_tag="PKZ52_05915"
     CDS             8087..>8626
                     /locus_tag="PKZ52_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNG59342.1"
                     /translation="MKILFLSHYFPPEVNAPATRTYEHCRQWAAAGHKVTVITCAPNH
                     PKGDVFSGFNNNLYHVSYVDGIRVVRVWTYITANEGFLKRTLGYLSYMFAAIIASFFV
                     GKQDLVLSTSPQFFNGLAGFFVSRINHSPWVLEIRDLWPESIVAVGAIRNTTIIKCLE
                     HIERFCYRKADHIIAVTQSF"
BASE COUNT         2371 a         2197 c         1878 g         2169 t
ORIGIN      
        1 cctcaagctc ctcaagctcc tcaagctcct caagctcctc aagctcctca agctcctcaa
       61 gctcaggatt gcctgaatcg gcgattcata aagatgtcat aagaagtatt tgcatttatt
      121 gccacaaaat aaaaggcgcc gccgaagatt cccgccttgt ttttaccaat ggcccgcagt
      181 attggaaaat agaagccgac aatcttgcac aattaactca attagcaaaa accattggca
      241 acgttgccat tttagaaaaa gtgcgcggtg cgagtttgca tggtggcggc attcaactaa
      301 cgcctgatgc ctatggttat agcgtcttaa aaacattttt agaggaggta accggcgcac
      361 aacccacagc tcaagcgccc acaaataccg tattggaatc cacacgggct acctatcgcc
      421 gcgccagttt gttgttaacg ggcaaagtgc ccgaagccta tccaagccta ataaatgatg
      481 ccgatctcac gacggcatta gataagttaa tggacaacgc ggagttttat gaatttgtaa
      541 aacgcggtgc gaatgatcgg ttactcactc gctccatcaa tagcacctta agcctggatt
      601 tttatttagc caattattat ccgatgtacg ctgccatatt agctagcagc ccacgtaata
      661 atggatatgc tcaagccgtt agccgagaaa ttggtgaatc ggtgctggaa cttattgcct
      721 acgtagtcaa aaataaccgc ccgtatacag aaatattaac ggccgattac accatggtgg
      781 ggcccaatac agccgttatt tttgagagca acctaagccc ccgcgcgggc gaatttttac
      841 cggccaaaga ccaggggcaa ctggtatcag ccacccaaaa tcaattgccc agcagtaatt
      901 gggacgctac aagcaaagca tttaccgagc ctgtaggcat attaaataac ctaggctatt
      961 tacagcagta ccaaactaat gccaccaacc gcaaccgagc gcgtgcccgc tggacgctgc
     1021 aacacttttt aggtattgat atagaaaatt ccagctcccg caatattgat gcaagcgtat
     1081 taattgacgt gcaaggtggc attccaacac gttttgcgtg caaggtgtgt cacgaccgca
     1141 tagatcccgt tgctggcact tttcagcgct atgggccgcg cggtatgtat cttgatggcg
     1201 catttggtat cgacacccta gacagcagct ataaacgcac agcgttttat aaaacagggg
     1261 acacctggta tcgatcgatg ttgccgccgg gttttgaagg taccgtgttt gacctgaata
     1321 atttaatcca agatcgcgac cctctaagag aactcgctga acacatagta caagatccca
     1381 aatttggtga aggcacagta aagttttggt ggcctgccct gtttggcgaa gatattttgc
     1441 cctaccaatt aaccagcgcc aatttagccc taagacaaac agatctaaat gaattcgcca
     1501 cacaatttag tagcgacaac tacaacttta aacatctgct gcgaacacta attctttcgc
     1561 cgtattttcg cagcataccc acgaaagaca atcgctacaa acaatctcgg cgtttgctaa
     1621 ccccggaaga attacacgat aaaacccagt ctctcgcgca ctatgcagat agccgtctac
     1681 tgaatgactg gctcattatt tatggcggta ttgattctta taacgtcgaa cagcggcaac
     1741 gacaattttc ccccatgatg taccaagttg ccaaacaaca cgccgcagac gcatcctgtg
     1801 aactggtaaa taacctgcgt gccgacacaa gcaacggcgc gcttacccaa cccaaacaga
     1861 tcagccaagg atttacgctc gccactagcg caactcaaga agtgactaca acaaacaccg
     1921 accatcaact gatactgcgc aataataact cggcgcccgc cgctttcagc atcctattta
     1981 ttagcaacaa tggcacagtc tcgcagtcag agcgcatcac actcagcggt aacactgcgt
     2041 ggaactcggc ggtacacaat tccgctgtca cctggcgcat tacgaatgaa tcaacagccc
     2101 cgatcaattt aaccgccagc agcatcagcc taaacaccca ggcattacgc acttatttta
     2161 gtgaattggc ttttgcattc tggggcgaga cagacgagtc aatgatcaat gaactgttgg
     2221 cattttattt tgccgcccgc caactacagg attacaacac cattaacacc tgtaacctag
     2281 gtagtgccaa tagtgatgtt ataaatacgg aattagccag ctggcgcatg gtttttattc
     2341 gcatgatgac ggactaccac tacttatatg aataacccta cacgacgcac ttttttacgt
     2401 accggcacgg cactgagtct acagtcatgg ctaaacccct gcatagccat agagcccaca
     2461 acggaaccgc tatttgtggt gcaattattt gctaacggag gtttagattg cgcaagcttc
     2521 tgcgacccca aagcaaatat caatggcacc attaataatt gggcaactaa actggatatt
     2581 aagcaagccg gcccgtttgc ttttgccccc attgcggata acgaaggatt ttttactcgc
     2641 caccaggata aaattctggt gattaatggc gttaatgccc aaaccaatgt gcattcagcg
     2701 ggccgcttaa ccagccttac cggccacaat aaaaatggct atcccagttt gtgcgccctg
     2761 catgcagccc aacacaatac tcaactgccc atgccacttt tggtgggtaa ctcatttgaa
     2821 acaggcggca tcattgccgc cacccaaatc aatggtgggc taattgaaat tttaaaaagg
     2881 cctacacaaa cagacaaaaa atatctaaac gccgctcaac agcagctaat cgataacgcc
     2941 cttaaccagc gggctgcgca aagctcagcc aaagcctttt atcgcgcagc cattcagggc
     3001 aaacaggact attttgctca aacagtagac ctgtatcaaa gcctaagcag cgatgaattg
     3061 ccgacaaaaa acacagtggt taaggatatg aaatttgccc tagcggcttt tgccgctggc
     3121 actagcatcg cctgcgacta tagcatcagt ggttttgata cccacacaga ccacgataaa
     3181 aatatgctgg tgccactgaa cacactgact tccgccgccg atgcagcttg gtattttgcc
     3241 gagcaattac aaattgatcg ccgcttatta gtgatcatca gttcagaatt tggccgcaca
     3301 cctttttaca acgacgttgc gggcaaagat cattggccgt ttcacagcgt cataattatg
     3361 aaaaaagatg ccacttggag tgggcgaatc gtcggccaaa cggatgnnnn nnnnnnnccc
     3421 cctcaccgcc gatcccaacg gcaccctatt agacatgggc cacatccaac tggcattacg
     3481 gcattattta aatattagta gcaccttaga taaaaaatat cccctcggct caaccctgtt
     3541 aacctcactg cttgcctaaa aataccccga ttaaattgtt ggtaactatt gagcacatct
     3601 tattagcctc ttaagcgcga tcggcaaatt aacagcttgt tgaaatacta ccttgcgagc
     3661 gccggcggcg ttaaattttg acaaaaatcc tcatctactg cgtgtcaact cggcaattgc
     3721 tcctgcattg ccagagcgtc gccgcctagg tatttcaacc agctgccagc ctgtgtgggt
     3781 ataagcgccg atttaaaacg cactggaaag agtcaatctg attaagtcgt cttgtcacat
     3841 acctgtcacc aatatcaatt aaaaataact cgatttctcg agggtgacac atgaaacaat
     3901 tgttacaaag tttacgcaat ggcgacacac tcatcaccga ggtgccagct ccatccgtgt
     3961 cccccctaca agtgcttatc cgcacccata ttagcgccat ttccaccggc acagagaaaa
     4021 tgctggtgga ttttggcaag gcgggtttgg tcagcaaaat taaacaacag cctgacaagg
     4081 tgcgcctggt tttagacaaa attaaaaccg atggcctgtt taccacgctg gatacagtac
     4141 gggcaaaact cgatgaaccc attgcgctgg gttattgcaa tgtcggcact gtcatcgccg
     4201 tcggcaatca ggtaaccgaa tttgccgtgg gtgatcgggt cgtcagcaac ggcaaccacg
     4261 ccgaaattgt ggcggtaaat aaaaacctct gcgctaaagt gcccccggcg gtggccgatg
     4321 aagacgctgt atttacagtg ctgggcgcca ttgcattgca aggcgtgcgg cttattgatg
     4381 cccgcatagg cgagacctat gtggtgatgg gtttaggctt aattggctta ttgaccgtac
     4441 agttgttaag agcgcagggt tgccaagtat ttgccctgga tttagccgcc gataaaattg
     4501 ccgccgccga agcactcggc gccaaaggca tattaattca agcgcataac gacagtgtgc
     4561 agaccattaa aaatttgcac ccaaatggcg tggatggcgt tttattaacc ctcagcacgc
     4621 aagcgtcaga acctgttgcc caggccgcgc atatgtgccg caaacggggc aaaattgttt
     4681 tagtgggcgt aagtgggcta catttatccc gggctgattt ttacgagaaa gaactgagct
     4741 ttcaggtatc ctgctcctac gggccaggac gttacgatcc taattatgaa gtgcagggcc
     4801 aggattatcc catcggattt gtgcgctgga cacaacagcg taatttcacg gcaattttac
     4861 aattaatggc tgacggcatt ttaaaagccc agcattacgt atcccatcgt tttgctattg
     4921 ataaagccga gcaggcttat gaattattgg gccaatcggg tgtaagtgcc attttattag
     4981 aatatcctca aaacaccctt gcaccagccc aaattattca ttacccccaa cacacgagtg
     5041 ctatcgcccc gacatcagca gttaaaatcg gttttattgg cagtggtaat tacgcgcggc
     5101 ggcatttaat gcccgctttt gccaaaacag gcgccgaatt tttagcagtt tcctgtcgta
     5161 gtggcgtaag cggggttaag gccgcagcgg attttaaaat tagccttgcc tgtaccgaca
     5221 atcaagtcat attcaatcat gctgattgta atttggtggt tatcagcacc caacacaaca
     5281 cccacggtcg cctcgtgcaa caggccattg ccgcgggtaa acatgtattt gttgaaaaac
     5341 ccctgtgctt aactcaagct gagctggact ccataagcac agccgtacac acatcagcca
     5401 atagcctgca aattaccgtt ggctttaatc ggcgtttttc taaattgagc caacacttaa
     5461 aaaaattagc tgcgtccaca ggtgaacccc tggtgatcag ctacagcata aatgcaggtt
     5521 ttatcgaccc atcccattgg agccaggatg tgaatatcgg cggcggccga cttttaggtg
     5581 aggtgtgcca ttttatcgat ttatgtcgct tttttgccgg atctaagata gtttcgagta
     5641 atgtacaggc gctacacaaa aatggccacc ctgcagatag ctggcaaatt ttgttgcgtt
     5701 ttgccaattg ctccatcgcc acgattaatt attttagcaa tgggcacaga tccctaccta
     5761 aagaaactat tgaggttaat accgctggca aatcacttaa aattgataac ttccgccgtt
     5821 taagctgcgt aggatttggc gctgggaagg aaattaaact gtggaaacaa gacaaggggc
     5881 agacccaatg tgcgcttgaa gttgtgcaga gcataaaaga cggcaggcct gcgcctattg
     5941 cgtttgcaga gctgcatgaa gtcatgcaaa cctgtttgga tctgtggaac caggtgcaaa
     6001 aatccgcgcc ataggtataa ctgcgctatt tcgggtttgc ttctaaatga atttttgcaa
     6061 gcactccaac aaagctcaca ccatgccatc caaaccatgc gccccaaaat aatcacgtcc
     6121 ggctacgggg cactggtaac cctggcacgc gcggcaaata ccctacggca cttaaaaatc
     6181 cgccagttgt acttttttct agtgcgccgg gttattaaac cgccctacag gcttacccag
     6241 cagacagggg ccccaaaccc tgcatttaat ttgctttatt ttgcagggca ccaggagtgc
     6301 tacgcggata atcgcctgac tttccttaat acaccctcac cttttccaat tcagaacctg
     6361 aactggcagc cagccgccat gagcaaatta tggcgctata acttgcatta ttttgactat
     6421 ttattggcgc ccagcgcaaa cgaagcgccg gcgaactgtt taaataacgc tgataaaaat
     6481 tcgttaatcc aaagctggct tgagcaaaac ccataccctg ctgaagacgc ttgggagccc
     6541 taccccacta gtttgcgtat tatcaactgg ataaaatatt ttcaacgcct tggcgcagat
     6601 ttaataccgc agcactggca ccacagcctt tatcagcagg ggcattattt aactcaccac
     6661 attgaatggc atattgatgc caaccattta cttaaaaata ttatcgctct ggtcatttgg
     6721 agccattatt ttaatgatac ctatgcgcgc cattggcagc agcgctccat taagcttttg
     6781 aaggcgatat gccaagagca attttttgcc gatggaagcc actatgagcg cagccccatg
     6841 taccatttta ttctggtgca ccatttgttg gacgcttata actgccttat caataacaca
     6901 ggcactgctc cgctctggct taaagaattg ctaaaccgtg cacttagcta ctgctgcaca
     6961 atcgtaaaac ccgatgggcg aattccttta gtcaaagata gcgcccacgg catagcgccc
     7021 ggcattgatg atctcaaagc ctatgcaaca ctattagata tttggccgca agaaaataac
     7081 acccgaccag taaaaggtga gttaacggcc tgcggctatt ttattgttaa atcggagcag
     7141 gactatattc tttttgatgt cggcaatctc tcccccagcc atcagcctgg ccatgcccac
     7201 tgcgatgcat tgcatatgga aattttctgg gatggcaggg atgtttttac agacgcaggt
     7261 gtttttaatt atgttgcaag ccccgagcga gattacgccc gctcagtacg cgcccataat
     7321 acagcctgta ttaatggccg agaacaacag gaactatggg gtgcatttcg cattgcgcgg
     7381 cgcggcgtta tgggcgatgt gacacacgac gaacacagct gcagcgctga gttttacccg
     7441 tatttttcac gcaacggtca cactggtcac aaaagatcca taaaaataga ctccagtgac
     7501 accatagaaa ttagcgaaca gctattgggc actggttgcg cccaggcaga gattttctgg
     7561 catttagctc cgggcctact cccgcagcgc aatcacaatt ctattgaaat ccgcaacgaa
     7621 aagcagattg ttgtagctgt cattaaactt caacacaaac aggttatttc tatagtcacg
     7681 caaagtgatt acttccctga gtttggtaag cgctgcagtc gtagctgtgt gacattacag
     7741 attaatgacg cgcagctgcc gatgacgctt aattaccaaa ttattaaagg ttcagcagcg
     7801 gccaacattt gttattaatt gaggctggtt ttctgccagc tatgttgtgt gcgcagcacg
     7861 cctggttgtt tgcacggcgc ggctggttgt gtgcaaggca agcctatggc aattgtgcag
     7921 cccccacctg cgggaaatag ttctgccgag caaaagtgcc ttaacgataa attaagtaca
     7981 tattacctaa aaccctacac actcgccgag ggcaaagtgg cagttacact cttgtaccct
     8041 ctggcctcat ttttaatgcg caatctacag cagcagaagg tgctttatga aaatattatt
     8101 cttatctcac tattttccac ctgaagtaaa tgcacctgct acgcgcacct atgagcactg
     8161 ccgccaatgg gccgccgctg gccataaggt aacagtcatt acctgtgcac ccaatcaccc
     8221 gaagggcgat gttttttccg gcttcaataa caatctgtac catgtctctt atgttgatgg
     8281 cattcgagtt gttagggtgt ggacctatat aacggccaat gagggttttt taaagcgcac
     8341 attgggttat ctctcctata tgtttgccgc cattatcgcc agttttttcg tcggcaaaca
     8401 ggatttagtc ctatcaacct caccgcaatt ttttaacggt ttagccggtt tttttgtcag
     8461 ccgcataaac cacagcccct gggtgctgga aattcgcgac ttatggcctg aatccatagt
     8521 ggctgtaggc gccattcgca acaccactat cattaaatgc ctggaacata ttgagcgttt
     8581 ttgctaccgc aaagctgatc atattatcgc tgtcacccaa tctttt
//
LOCUS       DAOQGS010000112         8616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3882_length_8616_cov_5.003271, whole genome shotgun sequence.
ACCESSION   DAOQGS010000112 DAOQGS010000000
VERSION     DAOQGS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_8616_cov_5.003271"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKZ52_05920"
     CDS             <1..978
                     /locus_tag="PKZ52_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNG59343.1"
                     /translation="REIQQVRTMMNQVADTDVTVLISGESGTGKEVVARNLHYHSPRR
                     DKPFVPVNCGAIPAELLESELFGHEKGAFTGAINSRAGRFELAEGGTLFLDEIGDMPL
                     HMQVKILRVLQEKTFERVGGNKTFHTDVRIIAATHRNLEDMIEAGEFREDLYYRLNVF
                     PIEMPSLKSRAEDIPLLINELVNRLENEQRGSIRFNSAAILSLCRHPWPGNVRELANL
                     VERMAIMHPFGVVGVQDLPQKYRHVEVEDDLEVETAPAQIPSVVRMTDTALLPEDGID
                     LKEYITGLEQSLIQQALDDSGGVVARAAERLMIRRTTLVEKMRKYGMQR"
     gene            1599..2714
                     /locus_tag="PKZ52_05925"
     CDS             1599..2714
                     /locus_tag="PKZ52_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59344.1"
                     /translation="MPDITPAVSYSPESSNQPYSVVIQSIGRASLASAAAVAKGLGIS
                     AADVVQCLYRAPAILVDQIEERVAAHLATLLGSMGFHADALPSAAAPPKDCELFDVAL
                     YMQDANRLGEAAERYAEFTGLPQADAVKMLLQPPGLALGSVSRATVRALADAMGDCVE
                     LVSSSQTHGLFDVFLADCPAVVKKRLLDDLSAAGIPVIADTGLIASGINRNQLEPLWQ
                     RHKTNSALRIVHQDFLRFDVTLLPTSQPLTAAQKDALARWVNIPHELTEEVAQAAPVA
                     LLESVAQRDLKPILEGFAQVDLPVRADLITFQVLGLKIIACTDLAALNRLLTSFNCTT
                     PVTRLPAALNEQFPEIHARVLHTALTRAGVQVEFIKN"
     gene            2725..3312
                     /locus_tag="PKZ52_05930"
     CDS             2725..3312
                     /locus_tag="PKZ52_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59345.1"
                     /translation="MTLAQETPARNAHEMHFAVAEYGKVMALQKNHPEALRHYREAIR
                     MAVSIKAPEVFFRHYTQCVLESLELTGAYGDVIEFCENADRHYKQLNITRAVHKKDWA
                     SVLERFAINLVKAGQIQNGKEAIRRALECAAEVPMPLAQELNQWLLRGLHVDNTRLLA
                     LQKKHHYFVVRPEQVNTAQAHSLESLNAPGRQISL"
     gene            3393..5201
                     /locus_tag="PKZ52_05935"
     CDS             3393..5201
                     /locus_tag="PKZ52_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNG59346.1"
                     /translation="MAEYDGLTPGQVLADASVAEFIKSLGIGIAEAQRALDENSVQQI
                     AEFIEPREGLGGKTLLDLGLSPAFYHYQHADITCSMQLSLRVQKDLSLGLNLNGSFSD
                     NTSNSSNSNSSETSSESGSSTRTESRTANVEITSASRGALVVGGQNFALNGATPAERI
                     RNLQNAITADNATGIDAVLFQLQGSDLTITENPDSPSVVTTPHSVAFIGGGFDRGIIR
                     IGQNQNTVFNLDDAPSAVAANTTAQADLAAYAAHVATTIAAQNYTTFHRAPSEPILTA
                     HFDTGRHDLRADDMDQLRLLAQFVTAVNMPITLQGFADRQRFEGMSDSGNRAANIALG
                     NNRAQEVKNFLLANGVPANLITLTPSTGSDAAPASPIDNQAFRKVDITTPGRTHHLVF
                     VHANGAEPNLNAVTPNMVGNSSSDNGFIYLYKPSPLNLTGGVTIDGTTFNYRSTAAGS
                     FAANSPEAYAKNLADDINANTAATLQASANANVVTVSKNGDKFQLTLVTSNNRNITLT
                     GSSGVTVTTQFSRSSSSNLTRQNTGNRAVAVGASLDVRFSRQFEMNVTGNSSISARLV
                     SIPAPPQFLETIRSFLNPANSATPSNNNPGNNGGGN"
     gene            5201..5668
                     /locus_tag="PKZ52_05940"
     CDS             5201..5668
                     /locus_tag="PKZ52_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59347.1"
                     /translation="MAETVEVTRALVTAPLPQIIEKLGLAIAQAQFALDTNSIELAKT
                     MAATEVKIGDDTYNLLSLGFMPSFYSFSEATVEAKLSFTMTESTETSVAVGVQAGINY
                     GVFMAAASIDVSYARKFSVSTEGASSIAARLVSLPAPDVFTQLIKRQFERPIT"
     gene            6134..6856
                     /locus_tag="PKZ52_05945"
     CDS             6134..6856
                     /locus_tag="PKZ52_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59348.1"
                     /translation="MASIGQELLNVPMGDMIRQMAFAIAEAQYKLDENSINVAEMMGG
                     LTTVYDEKGKPTFDDSRVFFGYEYMTVKEAQAYVLVDDALTGNNDLKLADALNELIAG
                     KQPTDIIRVPTRVSMMELGFTPVFYQFIDTIIEVKIAIKITRTSESSRTSKTTNTDKT
                     NTFRHKNSFWGNNGSASVNAQVSTSQVDATYASKYSYSAEGASLLRTKLSPVPPPPML
                     EERIRNLMDSETKRRKEAAATT"
     gene            6875..7438
                     /locus_tag="PKZ52_05950"
     CDS             6875..7438
                     /locus_tag="PKZ52_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59349.1"
                     /translation="MSIGTELLNAPFPEMIKTLGVSIAEAQYELDLVSMKIARLMAGY
                     APDPEPENGAEPPPSSATPERNMLVPLGDGKSYSLLELGFTPTFYQFVDTLIEIKISI
                     SMSRETNFNTSSTTVNANLKGSVGFFSASASMRVSTVSASYASKYQYSAEGSSLMRTK
                     LVPVPAPALLEERIRAMIAASNKPPTP"
     gene            7438..8364
                     /locus_tag="PKZ52_05955"
     CDS             7438..8364
                     /locus_tag="PKZ52_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59350.1"
                     /translation="MPGNSWQDRLGLRGTSPSTPAAPAAPASTAASEAAAPVPERLNV
                     LLQQRQQLEQRMQSLVGGGTGNLREAARFQVPSFAQRLAEQRRWEAERARPRSRSSET
                     SVTTGGPSSAPEVEAMRSGLRNFSQPVNDWLAGRDEDRRRLRDQARDRLSPLETLAEP
                     VRGARRQVQDANRQLRDLDQRLASEGLNEDRAALRELGADRLDAAERYLSRADEALGA
                     PRQVVDRLDDAWQNRSNQISGAMDRVGSYVDRSRQRLSSETGGSGDLFTRMQQNRERA
                     VQLRREQQLQERRDQARRDRARQRRRDLEDSE"
     gene            8361..>8616
                     /locus_tag="PKZ52_05960"
     CDS             8361..>8616
                     /locus_tag="PKZ52_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59351.1"
                     /translation="MSDNSQFVAESLAELLVAIAGGVREAQEALSQMPALDRLGRPMP
                     SYHLPYLDFQLQVDMDSTQTESGARLLRVLPLANSQKTTSQ"
BASE COUNT         2120 a         2351 c         2232 g         1913 t
ORIGIN      
        1 cgagaaattc agcaggtgcg caccatgatg aaccaggtgg ccgataccga tgtaaccgta
       61 ttaatttccg gtgagtccgg cacgggtaaa gaagtggtag cgcgcaattt acattaccac
      121 tcacccaggc gggataaacc ctttgtgcca gtcaactgtg gcgccatccc cgctgaatta
      181 ttagaaagcg aattattcgg ccatgaaaaa ggcgctttta ccggcgccat taattctcgc
      241 gctgggcgtt ttgaattggc cgaaggcggc actttatttt tagatgaaat cggtgatatg
      301 cccctgcata tgcaggtaaa aattctgcgc gtgttgcaag aaaaaacctt tgagcgtgtg
      361 ggcggcaata aaacctttca caccgatgtg cgcatcatcg ccgccaccca ccgcaattta
      421 gaagacatga ttgaagccgg tgaattccgc gaggatttat attatcgcct caatgtattt
      481 cccatcgaaa tgccttccct taaatcccgc gccgaggata ttccgctgtt aattaacgaa
      541 ctggtcaacc gcctggagaa cgaacagcgc ggctccatcc ggtttaattc cgccgccatt
      601 ttatccctct gccgccaccc ttggcccggc aatgtgcggg aactggccaa cctagtggag
      661 cgcatggcta taatgcatcc tttcggtgta gtgggcgtgc aggatttacc gcagaaatat
      721 cgtcatgttg aagtagagga cgatttagag gtggaaacgg cgcctgcgca aattcccagt
      781 gttgtacgca tgacggatac cgcgctactg ccagaagacg gcatagacct aaaagaatat
      841 atcaccggcc ttgagcagag cctgattcag caggcgctgg atgattccgg cggggtagtg
      901 gcgcgggccg ccgaacgcct gatgatccgc cgcaccacgc tagtggaaaa aatgcgtaaa
      961 tacggcatgc agcgctgaaa cgacagggat taatgaatag gctggaacgg aacctctggt
     1021 taaataaccg ctgattaagc cttaattcgg cggtgttttg cagacatcct ggaacagcca
     1081 ttgacaggaa gtctgctgct agagcgacag gaacgcgctg atgtgttttc aactgtccgc
     1141 gcatccaggg tttacaagag cgttaactgg aaattatcag gcgatagttt aatgttgcga
     1201 gccctcaata attcccccgc attgatgaat acaggttatt tcagacccgt catgaataca
     1261 tcagtgtaag ctcgtcaaaa aggtccctct ttttgacgcc atgagataac ctgtattcac
     1321 ccctgctgca ccgcgaatcg attctgccac ttaactaaaa ttccccaatt tgcctctccc
     1381 gcttaggaga gggtaataag ttgtgctgaa tgaaatagtt actttatatt ttccaccctt
     1441 cttaaaaacg ttcatccaaa gaaaattaac cgccaaattt agccctcccc agggcttatg
     1501 cttttgtgaa tagttcacag atttgtcatg atgttgggta tagagttcgc ctaatccgtt
     1561 aagcgatttt tcgccccgca tcagcgcatg aggttgttat gcccgatata acaccagctg
     1621 ttagctattc ccctgaatct tccaaccagc cttattctgt ggttattcag tccattggcc
     1681 gcgcgtcttt agcctcggca gcggcggtgg cgaagggttt gggtatcagt gctgccgacg
     1741 tggtgcagtg tttataccga gcgcccgcca tactggtgga ccaaattgaa gagcgtgtgg
     1801 ccgcccattt agctacatta ttaggctcca tggggtttca cgccgatgcc ctgcctagcg
     1861 cggcggcgcc gccgaaagac tgcgaattat tcgacgttgc cctgtatatg caggatgcaa
     1921 atcgattggg tgaggcggct gagcgctatg cagagtttac cggcctgccc caggccgatg
     1981 cggtaaaaat gctcctgcag ccccccggcc ttgccctggg ctcggtcagc cgcgcaaccg
     2041 tgcgcgcctt ggccgatgcc atgggcgatt gcgttgaact ggtgagcagc tcacaaacac
     2101 acggcctctt cgatgttttc ttggcagatt gccccgccgt tgtaaaaaaa cgcctattgg
     2161 atgatctgtc tgcggcgggc atacccgtaa tcgccgacac gggcctaatt gccagcggca
     2221 tcaatcgcaa ccaattagaa ccgctgtggc aacggcataa aaccaacagc gcgctgcgca
     2281 ttgtgcatca ggatttttta cgttttgatg taaccctatt gcccacaagc caacccttaa
     2341 ccgctgcgca aaaagatgct ctggcccgct gggtcaatat tccgcatgaa ttgaccgagg
     2401 aagtggccca agccgcacca gtggctctgc tagaatccgt cgcccagcgg gatttaaagc
     2461 ctattttaga gggctttgca caggtggatt tacctgtgcg cgcggattta attaccttcc
     2521 aggtgctggg tttaaaaata atcgcctgca ctgacctagc ggcgcttaat cgcctgttaa
     2581 ccagttttaa ttgcactaca cctgtcacac gtttaccggc ggctctaaac gagcaattcc
     2641 ccgaaattca cgcccgcgtt ttgcataccg ccctcactcg ggcgggggtg caggtagagt
     2701 ttataaaaaa ttagcggagc cattatgaca ctcgcccaag aaacacccgc gcgcaacgcc
     2761 catgaaatgc attttgctgt ggcggagtat ggcaaggtaa tggcgttgca aaaaaatcac
     2821 ccggaggccc ttaggcatta tcgggaggct atacgcatgg ccgtatctat taaagcgccg
     2881 gaagtgtttt ttcgtcatta cacccaatgt gttttggaaa gtttggaatt aaccggtgcc
     2941 tatggcgatg taattgagtt ttgtgaaaat gccgaccgcc actacaaaca acttaatatc
     3001 acccgcgcgg tacacaaaaa agattgggcg tcggtattgg agcgcttcgc gataaacctg
     3061 gttaaagccg gacagattca aaacggtaaa gaggcaatcc ggcgcgcact tgagtgcgcc
     3121 gcagaagtgc ctatgccgtt agcacaggaa ttgaatcaat ggctgctgcg gggcctgcat
     3181 gtagataaca cccgtttgct cgcattacag aaaaagcatc actattttgt ggtgcggccg
     3241 gaacaagtga acaccgctca ggcgcattca ctagaatcct taaacgcgcc gggccgtcag
     3301 attagcctgt aaaaaccgac gacgcagaaa tatttgtttt aaacaattgg cacagtgaat
     3361 tcatgtaaat aaactcaatt tggaggcccc caatggctga gtatgatggt ttgacccccg
     3421 ggcaagtgtt agccgatgcc agcgtggcgg aatttatcaa gagtttaggt attggcattg
     3481 cggaggcaca gcgggcattg gacgaaaatt cggtacaaca aattgccgaa tttattgaac
     3541 cgcgcgaggg tctaggcggc aaaacactat tggacttagg cctctcaccg gcgttttacc
     3601 actaccaaca cgccgatatt acctgctcca tgcaattgag tttacgggtg caaaaagatt
     3661 taagcctggg gctgaattta aatggttcgt ttagcgataa caccagcaat tcaagcaata
     3721 gcaacagctc tgaaacctcg tcggaaagcg gcagctccac ccgcaccgaa agccgcaccg
     3781 ccaatgttga aattacctcc gcctcacggg gcgccctggt ggtgggcggg caaaattttg
     3841 ccctcaatgg cgcgacaccg gctgagcgta ttcgcaattt acaaaacgct attactgccg
     3901 ataacgccac cggtatagat gccgtgttat ttcaattgca gggctctgat ttaaccatca
     3961 ctgaaaaccc cgatagcccc agcgtggtga caacacccca cagcgtcgct tttattggcg
     4021 gtggctttga tcgcggaatt attcgcatcg gccaaaacca aaatacggta tttaatctgg
     4081 acgacgcgcc ctcggcagta gcagctaata ccactgccca agcagattta gccgcctacg
     4141 ccgcccatgt agccaccaca attgcagcgc aaaattacac gacctttcac cgtgccccct
     4201 cagagcccat attaaccgcc cattttgata cgggccgcca tgatttgcgc gcggacgata
     4261 tggatcagct gcgtttatta gcgcaatttg tcacggcggt aaatatgccc attacgctgc
     4321 agggttttgc cgaccgccag cgttttgaag gcatgagcga tagcggcaac cgggcggcta
     4381 atattgccct cggcaataac cgcgctcagg aagtcaaaaa ctttttgctc gccaatgggg
     4441 tgcccgccaa tttaattacc ctcaccccat ccaccggctc cgatgccgca cctgcgtccc
     4501 ccattgataa tcaagccttt cgcaaagtgg atatcaccac tcctggcaga acgcaccatt
     4561 tagtatttgt tcacgccaat ggcgcagagc ccaatttaaa tgcggtaacg cccaatatgg
     4621 tgggcaatag ctccagcgac aacggattta tttacctgta taaaccctca cccttaaatt
     4681 taaccggcgg cgtcaccatt gatggcacta catttaatta tcgcagtacg gcggcgggca
     4741 gctttgcggc gaattcacct gaggcctatg ccaaaaatct agcggacgat attaatgcga
     4801 ataccgctgc gacactgcag gcatcggcca atgccaacgt ggtaacggtg agtaaaaatg
     4861 gcgataaatt ccaattaacc ttggtaacgt ccaacaaccg caatatcaca cttacgggca
     4921 gctccggggt tacggttacc acacaattct cccgcagcag cagttctaat ttaacccggc
     4981 aaaataccgg caaccgcgca gttgctgtgg gagcctccct ggatgtgcgt ttctctcgcc
     5041 aatttgaaat gaatgtcacg ggcaattcgt ctatttctgc gcgcttagtg tccataccgg
     5101 cgccgccgca atttttagaa accatccgca gttttttaaa ccccgccaac agcgctactc
     5161 ccagcaataa caaccccggc aataacggcg gaggtaacta atggctgaaa ccgtcgaggt
     5221 gactcgggcg ctggtaaccg cgcccctgcc gcaaattatt gaaaaactgg gtttggccat
     5281 agctcaagcg caatttgcct tggataccaa ttccattgag ctggctaaaa ccatggcggc
     5341 cacggaagta aaaattggcg atgacaccta caacctatta agcctgggtt ttatgcccag
     5401 tttttattcg ttttccgagg ccacggtgga ggcaaaatta agttttacca tgaccgaatc
     5461 cacggaaacc tcagtagccg tgggtgtaca agcgggtatt aattacggtg tatttatggc
     5521 ggcagcctcc attgatgtct cctacgcgcg caaattttcg gtgtccaccg aaggcgccag
     5581 ctccatcgcc gcacgtttgg tatccctgcc cgcaccggat gtgtttaccc aattaattaa
     5641 acgtcaattt gagcggccga ttacctaata cacctaaaca caccgagcaa tttattttgt
     5701 aactattcag tacccttatt gcccgctcgc cacgggcgga aaatttgggg gatttattaa
     5761 accggcggta tttattaacc cggcggcttt ttatgccggg ttaatagcca ggcgacagaa
     5821 tcaattcgcg gtgtagcggt ggagaataca ggttattgca gaccgtcaaa aagagggacc
     5881 tttttgacga gcctacacgg atgtattcaa ggcgtgcctg caataacctg tattcactgc
     5941 tgcgggcggg ggattgaggg ctcgccgcac tagagcgctg acctgatagt ttccagcaag
     6001 tgctgggaat tgcgtccggt tacactataa aagttctgca tagttatttt gttttttcaa
     6061 ttttttaccc atagccgcag taataatttt tacccatagc ccccaggggt ttacttaaca
     6121 agaggacaat tttatggctt caatcggtca agaacttttg aatgtaccca tgggggatat
     6181 gattcggcag atggcgtttg ctattgccga agcccaatac aaactggatg aaaactccat
     6241 caatgtggcg gaaatgatgg gggggttaac caccgtttac gacgaaaaag gtaaaccaac
     6301 ctttgacgac agccgagtat tttttggcta cgaatatatg acggtcaaag aggcccaggc
     6361 ctatgtctta gtagacgatg ccttgacggg caacaacgac ctaaaactcg ccgacgccct
     6421 caatgaatta attgccggca aacaacccac cgatattatc cgcgtgccca cccgcgtatc
     6481 catgatggaa ctgggattta cgcctgtgtt ttatcaattt atcgacacta ttatcgaagt
     6541 taaaatcgcc atcaaaatta cccgcaccag cgaaagctcg cgcacgagca aaacaactaa
     6601 taccgataaa accaacactt ttaggcataa aaatagtttt tggggcaaca atggcagcgc
     6661 cagtgtaaac gcacaggtga gcacatccca ggtggatgcc acctatgcgt ctaaatattc
     6721 ctactccgcc gagggcgcaa gtttattgcg cactaaactc agccccgtgc cgccgccacc
     6781 gatgctcgaa gagcgcattc gcaacctgat ggatagtgaa acgaaacgcc gcaaagaagc
     6841 cgccgcgacc acctaacact taggggatta agctatgagt atcggcacag agttattaaa
     6901 cgcgcctttt ccggagatga ttaaaaccct cggtgtgagt attgccgagg ctcagtacga
     6961 attggatttg gtgtccatga aaattgcgcg cctaatggca ggctacgcgc cggacccaga
     7021 gcctgaaaac ggcgccgagc cgccgccctc aagcgccaca cccgagcgca atatgttggt
     7081 gcctttgggg gatggcaaga gttacagcct gttggagctg ggctttacgc cgacgtttta
     7141 tcaatttgtc gatacgctga ttgaaattaa aatttccatt tctatgtcac gggagactaa
     7201 tttcaatacc tcaagcacca cggtaaacgc caacttaaaa ggcagtgtgg ggtttttctc
     7261 ggcgtcggcc tccatgcggg tttccacggt tagcgccagt tacgcctcga aataccagta
     7321 ctcggcggag ggcagctccc ttatgcgcac caagctagta cccgtgcccg ctccggccct
     7381 gctggaggag cgcatccgcg ccatgatcgc cgcctccaat aaaccgccaa ccccctaatg
     7441 cccggtaatt cttggcaaga tcggctcggc ctgcgcggca ctagccccag cacaccggcc
     7501 gcccccgcag cccccgcctc cacagcggcg agcgaagcgg ctgcgccggt gccggagcgg
     7561 ctaaatgtac tgctgcaaca gcgccagcag ctggagcagc gcatgcagag tttggtgggc
     7621 ggcggcacgg gcaacctgcg ggaggcggcg cgctttcagg tgccgagctt cgctcagcgc
     7681 ttggcggagc agcgccggtg ggaggccgag cgcgcccggc cgcgcagccg cagcagtgag
     7741 acctcagtaa ccacaggcgg gcccagcagt gcgccggagg tagaggccat gcgctcaggc
     7801 ctgcgcaact ttagccaacc ggtgaatgac tggctggcgg ggcgagatga ggatcgccgc
     7861 cgcctgcggg atcaggcgcg ggacagattg agcccacttg agaccctggc cgagccggtg
     7921 cgcggtgcac gccgccaggt gcaagatgcc aaccggcaat tgcgggactt ggatcagcgc
     7981 ttagccagcg agggcctcaa tgaagaccgc gccgccctgc gggagctggg ggcggaccgc
     8041 ctggatgcgg cggagcgtta tttaagccgg gccgacgaag ccctgggcgc gccgcgccaa
     8101 gtggtggacc ggctggatga tgcctggcaa aaccgcagca accaaatcag cggcgccatg
     8161 gatcgggtgg gcagttatgt ggatcgctcc cgccagcgcc tctccagcga aaccggcggc
     8221 tctggggatt tattcacccg catgcaacaa aaccgcgagc gggccgtgca actgcgccgc
     8281 gagcaacaac tgcaagagcg gcgcgaccaa gctaggcggg atcgcgcccg gcaacgacga
     8341 cgagaccttg aggacagcga atgagtgaca acagccagtt tgtggccgaa tccctagccg
     8401 aactgctagt ggctatcgca ggcggtgtgc gcgaggccca ggaggcccta agccaaatgc
     8461 ccgcgctgga ccgcttgggc aggcccatgc ccagctacca cctgccctat ttggatttcc
     8521 agctgcaggt ggatatggac agcacccaga ctgaatccgg tgcccgcctg ctgcgggtgc
     8581 tgcccctggc caacagccaa aagaccacca gccaac
//
LOCUS       DAOQGS010000113         8569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3921_length_8569_cov_4.027484, whole genome shotgun sequence.
ACCESSION   DAOQGS010000113 DAOQGS010000000
VERSION     DAOQGS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_8569_cov_4.027484"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKZ52_05965"
     CDS             <1..856
                     /locus_tag="PKZ52_05965"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNG59352.1"
                     /translation="SDLVWDADLGFEGATSSWTVYSLKSSGAGVIASFLAGAFPIQEV
                     ELSQQDKWLYSLEASITWQQSNTNKLRAALGYFAFDNIVGEKNALDSKLTDYSAPKNM
                     QRGNSVFNIRNSSAPSSADELFGLAADFNDLDLILSYDIPVSEGRHLIVTADYVTNLG
                     YDSDPVASFMNDAAYAKQTDGYKVDVLWGVPKAFKRGEWNVTIAYRHLERDAVLDAFT
                     DSDFLLGGTDNKGFVLSGEYSVLDNTNIKLSFLSASSINPVGKSKDANNPIDFDSDSV
                     QLDFNVKF"
     gene            869..1465
                     /locus_tag="PKZ52_05970"
     CDS             869..1465
                     /locus_tag="PKZ52_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59353.1"
                     /translation="MINKYFAALSLVVILVPGAYAAGDEQKAALAKSQYMLRQLTAEK
                     SEMEKKNKELDEVNKTLKAQLDKVQAELASKNAQLAQWQTKAQEQQETLAKTQQESGQ
                     QQKELKRSAELINHQKTNIESRLQTQRANLSLCMENNRKLYDINKELVANYQNKGFWA
                     VAKQKEPFTGKQQVEIEKLAQEYQYRLDDAVVIEQGLE"
     gene            2022..4733
                     /locus_tag="PKZ52_05975"
     CDS             2022..4733
                     /locus_tag="PKZ52_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNG59354.1"
                     /translation="MKSLAKLPILAATLLACALVTACGSGNKSTSAAPSSSSSTSSSG
                     STKPIPVVNKIAVKVTGLVGKLELSNGSDLLTITNNGEAQFAKGVEVGISYNVQIVSQ
                     PENQLCSVLYGRKLMPAGGNLPVEITCSDGFGVGLDITKPADLSLKNLQVTSNYQRLG
                     GDNDPALTIDSKIKTSQNSFVALKNAQGDKTFYFSYVYAAYQTRLKLDPEHTALALLV
                     MEPIISDALNSHKLSVAAFFDLIAPLNAQKNRQLDGDLKKLADEIGRQVDKNADIFSA
                     SASLNPLIEKALIQAIDKIANLSAIVAPASAAAAADTGVEFSVQALSDSKVKVMLKNT
                     RPRVVAINGLKDQTTPLILDGQASGFLEQTVEAGKFYTLNASVEGPGKLGSTKAAATA
                     YQAAVARSSLIDYFMPNLVYFSGGKNLSADLALDCLNDGHNKDLSARAAGVWASVQAS
                     LEQDNYYKPYHTLADKYRAYWAEPTNMDDLLSCASLGLSGLSQGEKQIAKIHLTKLFT
                     GFNALRDPAGVKEDLFRTPKQSALVNAIVNSAAQSTWSYTNAFTLDIKAPAQVDDEIE
                     AEFSARCLDAAKNEVSCSLSWTLEDTNISGKAIKYLFKKATNQVVNLVATGADGAQVS
                     QAIAVGVQRKGSAIWVGKKDAAVALDTSKPLEFDSVFVGTKLPQTLVIKNTGYAPLVI
                     NSIAVEGAGFSVNLTEVTIEAGKSRSFEVVFSPQEVKNYSGVVSIGSNDKTVPSIKLS
                     FTGVGAPAVPIGKYAVVEDQITREEVVALTNETAISGDEEFAQVRLFGNAKSIFPHIS
                     LKLSNFAGPGTYSLYDTATDVCLALYAAEKDFTQQYCTGEKEGGSLVVTEISTELYQV
                     DYDFSAANCSKFIPTDGKPCTQKVIQLRGSTKVEKSVLIPVSTSLSK"
     gene            5343..5708
                     /locus_tag="PKZ52_05980"
     CDS             5343..5708
                     /locus_tag="PKZ52_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59355.1"
                     /translation="MIRYSISNTLGKLMPFHDRSYKGASLMPAVLDSAEELLRYFHLD
                     AEQLNKIINEGVDDPQKRVAEEDLEMLVLGHFNEQYIQQRLAELCEGPVESNQIHQAL
                     RDYLVRKYICAASHNLTTA"
     gene            5735..6217
                     /locus_tag="PKZ52_05985"
     CDS             5735..6217
                     /locus_tag="PKZ52_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59356.1"
                     /translation="MQFRTVLLTAALVGVSQLAAAATVDIAASGSDAWSKTVVGTYVK
                     NTKIRFSESGKYTINLQDTDFGANFEMLGAMISSPSTKYVDMVLSPEQSFKQRNFTID
                     KAGDYFLSVFAITDSIKNPATFSLSVSAVPLPSAVIFMSSALMGLVAFMRRRAGQVQA
                     "
     gene            6412..7005
                     /locus_tag="PKZ52_05990"
     CDS             6412..7005
                     /locus_tag="PKZ52_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNG59357.1"
                     /translation="MFAVAKKNLWLHFGCWILAAASYAAEPVATTANTNNKDAYFLQA
                     GDVIELLVWKEPDLSREILIGPDGFISIPLIGAVEAKNKTLDELRLEIKKRLSTVVNE
                     PSVNISLRGVAGNKIFILGKVARPGEYPLTQPVDVLQALSKAGGVTPFADASDIKIIR
                     RTGQDQAVIRFNYNQVERGKHLEQNVTLASGDTILVP"
     gene            7109..8185
                     /locus_tag="PKZ52_05995"
     CDS             7109..8185
                     /locus_tag="PKZ52_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59358.1"
                     /translation="MLSRLQYQDNVTLSEANPIADTSAQLSLNSRLWRKTQTQESELS
                     IAIKDDNYAEHSEFEQDRRSLSLDHIIKQELGSYVFKGDIDRSTTLGNGFESGEFVRR
                     NIAVNTRGFNASANRYVSEFISASISGGVTQARYQHVLNSDSQDYNDKQYSAAMSYQD
                     ADIANWQLSLYTDLLDQLQSKLTVDTTGIALQRNYKWSDLWSLSGKLGRRKTQFAGRT
                     FFGGQFTQENYGRVSSLDIKRAGELNTWGIGASEDLSPRVNGVIDETQRLGIWWETKP
                     SALATLKLNISHVQRKPINTAFFSDDATRYNTLSTGWQYAISETLSTDIQLRWVERAI
                     TSRANHDQAESGTASIGLRWQVHP"
     gene            8210..>8569
                     /locus_tag="PKZ52_06000"
     CDS             8210..>8569
                     /locus_tag="PKZ52_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNG59359.1"
                     /translation="MANAQFDIGELKRILNRRKKPALWVGVGVLIIGILVAILVPPTY
                     VSSSTILIEQQEIPQDLVRSTVTSFADQRIQSITQRTMTFANLSELIKKYDLYADDRR
                     SEPMEAIIEYMRRDIKTK"
BASE COUNT         2388 a         1830 c         2065 g         2286 t
ORIGIN      
        1 cagtgattta gtatgggatg ccgatttagg ttttgaaggt gcaacgagca gctggactgt
       61 gtattcactt aaaagctcag gggccggtgt aattgcatca ttcttagccg gtgcatttcc
      121 tattcaagaa gtggaattat cgcagcaaga caaatggcta tatagtcttg aggcgagtat
      181 cacctggcag caatctaata ccaataagtt gcgcgcagct cttggctatt ttgcattcga
      241 caatatagtg ggcgagaaga acgcgctgga tagtaaattg accgattata gtgcaccgaa
      301 aaatatgcag cggggcaaca gcgtatttaa tattcgcaac tcctctgcgc cttcatcggc
      361 ggacgaattg ttcgggcttg cagccgattt taacgacctt gatttaattc tttcctatga
      421 tattccggtg agtgaagggc ggcatttaat tgttaccgct gattatgtta ctaatctcgg
      481 ctacgactca gatcctgtgg catcgtttat gaatgatgca gcctatgcaa aacaaaccga
      541 tggctacaaa gtagatgtac tgtggggtgt gccgaaagcc ttcaaacgcg gcgaatggaa
      601 tgtgactatc gcgtaccgtc atttagagcg agatgcggta ttagatgcct ttacggattc
      661 cgacttttta ttgggtggca cggacaataa aggttttgtg ctctctggtg aatattctgt
      721 gcttgataac acgaatatta agctaagctt tttaagcgca tcgagcatca atccagttgg
      781 taagtctaaa gatgccaaca atccaattga ttttgatagc gatagtgtac aacttgattt
      841 taacgtgaaa ttctaaaagg ggatggttat gatcaataaa tactttgcag ctttaagtct
      901 tgtggttatc ttggtgcctg gcgcttatgc ggcaggtgat gagcaaaagg ctgcgctagc
      961 taagtcgcag tatatgctgc gccagttaac agcggaaaag tcagaaatgg agaaaaaaaa
     1021 taaagagttg gatgaagtaa ataaaacact aaaagcccaa ctggataaag tgcaagctga
     1081 gctagctagt aaaaatgccc agcttgccca gtggcaaacc aaagctcagg agcagcagga
     1141 aaccttagct aaaacccaac aggaaagtgg tcagcagcaa aaagagctga agcgctcagc
     1201 agaattaatc aatcaccaaa aaaccaatat cgaatcccgc ctgcaaactc agcgcgccaa
     1261 cctcagcctg tgtatggaaa acaaccgtaa gctctacgat ataaataaag agctagtagc
     1321 caattaccaa aataaaggtt tttgggccgt ggctaagcaa aaagaaccgt tcaccggcaa
     1381 acaacaggta gaaattgaaa agcttgccca ggaataccaa taccgccttg acgacgcggt
     1441 ggtgattgag cagggtttgg aataattttt caatgatact atttgaatta aaaagaaaat
     1501 tcacccaagt ctgtttgccc tctgctcaat cttgctaaga agatttcggg gcgcccattt
     1561 tgatcgcaca agcgaattat gccaaaggat ttggcatcaa ggtgctgtac tagatcccct
     1621 ggttacctgt agatcgcaac gtgcatggat agccatgctg atcatttaaa tgcctattct
     1681 ggcataggtt ggaagagcgc agcgacttcc gacataaatc ccgtcgagcc ctcgtagcac
     1741 atagaattca tgctggcgga tcggcgccta aagtgctgcg cttgtccaat aatttcgcat
     1801 caaaacgctt aaccaaatga cattgcctcc aaaggaggga attcaaaggg ctacctgcat
     1861 gcgcgaatcc ctaaattaat gacctaacta cccatgggat cgccttacac ctgtgctgtc
     1921 gtcggatttt ttgcgcgaca aactgacgac gaagtgtcac acaatcttca ttaaatttaa
     1981 ttacgctttg ttcattcgtc attggcttaa gcgttactct catgaaatca ttagcgaaac
     2041 ttcctattct ggcagccact ttactcgcct gcgcacttgt tacggcctgt ggcagcggta
     2101 acaaatccac ttctgctgcg cctagttcca gtagctccac cagctccagt ggcagcacta
     2161 aacctatccc tgtggtcaac aaaattgcgg ttaaggtgac agggcttgtt ggcaagcttg
     2221 aattgagcaa tggcagcgac ttattaacca ttaccaacaa tggtgaggct cagtttgcca
     2281 agggtgttga agttggtatt agttacaatg tgcaaattgt ttctcagcct gaaaatcagc
     2341 tgtgtagtgt tctctatggc cgcaaattaa tgcccgcagg cggcaatctg ccggttgaaa
     2401 ttacctgctc tgatggcttt ggggtggggc tggatataac caaacctgcc gatttaagcc
     2461 ttaaaaatct acaagtaacc agtaactatc agcgcttggg cggggataat gaccctgcct
     2521 taacaattga tagcaagata aaaactagtc aaaatagttt tgtggcttta aaaaatgccc
     2581 agggcgataa aacattttat ttcagttacg tgtatgctgc ttatcaaacc cgtcttaaat
     2641 tagacccaga gcacacagcc cttgccctgt tggtgatgga gccgattatc agcgacgcat
     2701 taaacagcca taagctcagc gtggcggcat tttttgattt gattgcgcca ttgaatgcac
     2761 agaaaaaccg ccagttagac ggcgacttaa aaaaactcgc cgatgaaatc ggccgccagg
     2821 tcgataaaaa tgcagatata ttttctgcat ccgcaagcct aaatccatta atagaaaagg
     2881 cgttaattca ggctatagac aaaattgcca acttgtccgc gattgttgct cccgccagcg
     2941 ctgcggctgc agcggatacg ggtgtagagt tttccgtgca ggctttaagc gatagtaaag
     3001 ttaaagtgat gttaaaaaac acacgcccgc gcgtggttgc aatcaatgga ttaaaggatc
     3061 aaaccacacc attgatactg gacgggcaag ccagcggctt tttggagcaa accgttgaag
     3121 ctggcaaatt ttatacgctt aatgcaagcg tagaaggccc tggcaaactc ggcagcacta
     3181 aggcggctgc cactgcttac caagcagccg tcgcgcgcag cagcttgatt gattatttta
     3241 tgccaaattt ggtttatttc agtggcggca agaatttatc cgccgatttg gcgctggatt
     3301 gtttaaatga cggccacaat aaagatttat cagcccgtgc agctggagtt tgggctagtg
     3361 tgcaggccag tttagagcag gataattatt acaagccgta ccatacccta gctgataaat
     3421 accgcgccta ctgggcagaa ccgaccaata tggatgattt attaagctgt gcaagccttg
     3481 ggttgtccgg cttaagtcaa ggggaaaaac aaattgccaa aatccatttg accaaattat
     3541 tcactgggtt taacgcgctg cgcgatcctg ccggtgttaa agaggactta tttcgcaccc
     3601 ctaagcagtc agccttagta aatgccatag ttaattccgc tgcacaaagc acatggagtt
     3661 atacaaacgc ttttactctg gatataaaag cgcccgctca ggttgatgac gaaatagaag
     3721 ctgagttttc tgcccgctgt ttggacgcgg cgaaaaacga ggtcagttgt tcactcagtt
     3781 ggaccttaga agatacaaat attagcggca aggctattaa gtatttattt aaaaaagcga
     3841 ctaaccaggt agttaacctt gttgctactg gcgccgatgg cgcgcaggtt tcccaggcga
     3901 ttgcggtggg tgtacagcgt aaaggctcgg caatttgggt gggcaaaaaa gacgcagcgg
     3961 ttgcgctaga taccagtaag cccttggagt ttgattcagt atttgtgggc actaagctgc
     4021 cacagacgtt agtaattaaa aatactggct atgcaccctt ggtaatcaac tcgatagccg
     4081 tggagggcgc aggctttagc gtgaatttaa ctgaggtgac tattgaggca ggcaagtcgc
     4141 gaagttttga agtcgtattt agcccccaag aggttaaaaa ttacagcgga gtagtatcta
     4201 taggatcgaa cgataaaacc gtacccagca ttaaactatc cttcaccgga gtgggtgcac
     4261 cagccgtgcc tattggtaaa tatgctgtag tggaggatca aattacccgc gaggaggttg
     4321 tggcacttac taacgaaaca gccattagtg gagatgagga atttgcccaa gtgcgactgt
     4381 ttggcaacgc aaaaagcata ttcccacaca tttcattaaa gttgagcaac tttgctggcc
     4441 ctggtactta ctcgctttac gatactgcta ctgatgtctg tttggcgctc tatgctgcag
     4501 agaaggattt tacgcaacaa tattgcacag gcgagaaaga aggtggcagc ctggttgtta
     4561 cggaaatttc cactgaactt taccaagtgg attatgattt ctccgcagca aactgttcca
     4621 aatttattcc tacggatggt aagccctgta cccaaaaagt gattcaattg cgcggtagca
     4681 ccaaggttga aaaatccgta ctaattcctg ttagcacaag cctgagtaaa taactctttc
     4741 tatcaaaaat cctcaagttc ctctggtctg taaaacaggc tggaggagat ctaccgaagc
     4801 gtgctttatg cacacccttt ttatttgccg gtaaaaatgc attcgcatat cgataaataa
     4861 acggttccta cagaatttat cactcgctcc gcaatgcttt ggcggctgag tatttgataa
     4921 atgacccttt ggcccttaag agggcctttt ttatgcccaa tcgatcactg cgctcgatgg
     4981 ctgcctaact ctttccctct cacgcatcct gtgcctcccc tttaaaacct cttattcgtc
     5041 tttgccgatt cgcgcaatct gctttttgtg ctgcgcttat ttttaagtgc aactaaacgc
     5101 acataaataa atatgctttc gtcataaaaa ccgcactaaa acgtcaaaaa tatttagtca
     5161 aaatgtaaac caatctgcca gcaaaagtgc ttatagtgtg catacacaat acctactata
     5221 actatataat cttgaggcac aaaatgcaga cgaaacgtaa gaatatatcg gtgcgaatga
     5281 gttcttggga gcagaagcgg gtgcgagaga tagcggagcg tttgggagta aaggagtcag
     5341 acatgattcg ctattccatc agcaatactc tgggcaaatt aatgcccttc cacgatcgct
     5401 cctataaggg cgcaagcctt atgccggcag tgttggatag cgcggaggag ttgctacgct
     5461 atttccatct ggatgctgag cagctcaata aaattattaa tgaaggcgtt gacgacccgc
     5521 aaaagcgagt tgccgaggag gatttagaaa tgctggtgct cgggcatttt aacgagcaat
     5581 acattcagca gcgccttgct gaactctgtg aaggcccggt ggagagcaat caaatacatc
     5641 aggcactgcg cgattatctt gtgcgcaaat acatctgtgc cgccagccac aatttaacta
     5701 ccgcttaatt tttattaact ttggaggatg ttttatgcaa tttcgaactg tacttttaac
     5761 tgctgcactc gttggtgtta gtcaattagc tgctgctgcg actgtggata tagccgctag
     5821 tgggtctgat gcttggagta aaaccgtagt aggtacctat gttaaaaata ctaaaattcg
     5881 cttttcagaa tcaggtaaat acaccatcaa tctgcaggat acggattttg gcgctaattt
     5941 tgagatgctt ggggccatga tctcctcacc atcgacgaaa tatgtcgata tggtgttatc
     6001 gcctgagcaa agttttaaac agcggaattt tacaattgat aaagcgggtg attatttctt
     6061 aagcgtattt gctattaccg actctattaa aaaccccgcc acattttctt tgtcggtgag
     6121 tgcggtgcct ttgccttcag cggtgatatt tatgtcctcc gccctgatgg ggttggtggc
     6181 ctttatgcgc cgccgggcag ggcaggtaca ggcctaggtt gtgtgctcta gacaaaaagg
     6241 cctgctgcgt tgtgtgtttg gctcatgatt ttaggggaaa ggaggccggc gtcttaaagt
     6301 attggctttg gcgctgactg cccttggttt tcggccagtc gtaggcgcct tgcaatatta
     6361 agttcatata gccaggttag agtgtcataa atgaaacact tggattcact tatgtttgcc
     6421 gttgcaaaaa aaaatctctg gcttcatttt ggttgttgga tattggccgc tgcaagttac
     6481 gccgccgaac ccgtggcaac gactgccaac actaataata aggacgcgta ttttttgcag
     6541 gcaggggatg tgattgagct gctggtatgg aaagagcccg atctttcgcg ggaaatatta
     6601 ataggcccgg atggtttcat tagcattcca ttgattggag cagtggaggc caaaaataaa
     6661 actttggacg agttgcgttt ggaaattaaa aagcgcttat ccacagtggt aaatgagcct
     6721 agcgtgaata ttagtttgcg cggtgttgcg ggcaataaaa tttttattct cggcaaggtt
     6781 gcgcggccgg gggaatatcc gctaactcaa ccggtagatg tattgcaggc tttgagtaag
     6841 gccggtggtg ttacgccctt tgccgatgcc agcgacatta aaattattcg ccgcaccggg
     6901 caggatcagg cggtgattcg ttttaattac aatcaggtgg agcgcggaaa acacctggag
     6961 caaaacgtaa ccttggccag tggcgatact attcttgtgc cttaaatata ttatcctgcg
     7021 gcgcccttac ctgtgtgtgt tacgtgccag caccttatct gttgtggcta tgaactgtgt
     7081 tgctgcagat attgattggg ctgcagatat gttgtcgcgg ctacagtatc aagataatgt
     7141 gactttatcg gaagccaacc ccatagcgga tacatctgcg cagttgtcgc taaattcacg
     7201 gctgtggcgt aaaacgcaga cacaggaaag tgagttaagt attgcaataa aagacgataa
     7261 ttatgctgaa cacagtgagt ttgaacagga taggcgcagc ctatctcttg atcatatcat
     7321 taagcaggag cttgggagtt acgtatttaa aggagatatt gaccgctcga caactctggg
     7381 gaatggcttc gaatcgggtg agtttgtacg gcgtaatatt gccgttaata cgcgcggatt
     7441 caatgccagt gccaaccgtt atgtaagtga atttatatct gcaagtataa gtggcggtgt
     7501 gacacaagcg cgctatcaac acgtattaaa tagcgatagc caggattaca acgataaaca
     7561 gtattccgca gccatgagtt atcaagatgc cgatatcgct aattggcaat tatccttgta
     7621 tacggattta ctggatcagt tgcaaagcaa gttgacggtt gatacgaccg gtattgcgct
     7681 gcagcgcaat tataaatgga gtgatctttg gtcgcttagc ggcaaattgg gccgccggaa
     7741 aacacagttt gctgggcgta ctttttttgg ggggcaattt acccaagaaa attacggccg
     7801 ggtatcctcg ctggatatta agcgggcagg tgaattaaat acttggggaa ttggagcaag
     7861 tgaagacctc tcgccccgtg tgaatggcgt tatagatgaa actcagcggt tgggtatttg
     7921 gtgggagacc aaaccatccg cgcttgctac cctaaaatta aatatcagcc atgtgcaacg
     7981 caaacctatt aacacagcgt tttttagcga tgatgcaact cgctataaca ccctcagcac
     8041 cggttggcaa tatgctatta gtgaaaccct gagcacggat atccagctgc gatgggttga
     8101 gcgcgcaatt acttctagag caaatcatga tcaggctgaa agtggcacgg cctctatagg
     8161 cctgcgctgg caagtgcacc cttaatattg ataaatggca aaaaattgta tggcgaatgc
     8221 gcaatttgat ataggtgaat taaaacgtat tttaaaccgc cgcaagaagc ctgccctgtg
     8281 ggttggggtg ggggtattaa ttataggcat tttggtagcc attttagtgc cgcccaccta
     8341 tgtgtcctct tcgactattt tgattgaaca gcaagagata ccacaggatt tagtgcgctc
     8401 cacagttacc agttttgccg atcaacgcat tcaatccatt actcagcgca ctatgacctt
     8461 tgccaactta agtgagctga ttaaaaaata tgacctttac gcggacgatc gaaggtctga
     8521 gcccatggaa gccattattg aatatatgcg gcgggatatt aaaaccaaa
//
LOCUS       DAOQGS010000114         8486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3962_length_8486_cov_4.838809, whole genome shotgun sequence.
ACCESSION   DAOQGS010000114 DAOQGS010000000
VERSION     DAOQGS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3962_length_8486_cov_4.838809"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1798
                     /locus_tag="PKZ52_06005"
     CDS             173..1798
                     /locus_tag="PKZ52_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG59360.1"
                     /translation="MKSLMAMLNLQTNVQVISFVAVVSLALLVVSNAVSVAAQWLLLT
                     FSMEIGGSVQGRLFASLISRDYLFHKTNDYSASIPKISQDTLRFLYMVLQPILQLFSN
                     GFVVVIILGGIVALNPTVAFVAGSVLSFAYAGTYYYLKKRTARAGDLLTQKTAATHKV
                     LNEAFVGVKEIKLVGIERQFVDKFNQVNHQGMQAATFLSLAVDIPRYVIETIALSAIF
                     ISAIFALMAGIEPSSILPILSLYAVAGYKLLPTMQQVYKAVSSISAHGSVALDIEREI
                     AQCSPEPIATELKTSKNRSIGLAVEVKSVSYQYPNTDQYALQGVSVQFCFGKINTIAG
                     HSGSGKSTLLDIALGLLTPTSGQLLVGGEPISNQTARAYQQRIGFVPQHVFILNDSVA
                     RNVAFGEAIEHFDAERLTNSLKLAGAMEFVSRLPEGVNTVLGQDGKLLSGGQRQKIAI
                     ARCLYRNSDILFLDEPTSALDIDAEFEFMQVLEALKARYLIIIISHRPSAIRYSDFIC
                     LMKDGVIEAAGSFDELKQCSDSFRNLIEKSSME"
     gene            1805..2872
                     /locus_tag="PKZ52_06010"
     CDS             1805..2872
                     /locus_tag="PKZ52_06010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNG59361.1"
                     /translation="MAPNNLKIVYFFNHPTAEDERKIASGEIPSDRLYAMVELKRRGW
                     QVDACDERLSDFEYKMRPWMSWINWACIKKAMQFDVWLVKDNFSFILSILARLFGKKL
                     LYIDSLFHYPKSRLRRASIWLNVRLAQQIGVYSHFQADFWSRKMSVSRAKFRVFSYTV
                     DHEFYLQGFANAQAHTEDEAFVLATGRDMGRDFATLIEATARLGIGLKLVTLPYLLPK
                     NIAEYSHVQILQRISYAELFALYRDAAVVAVPLRDNIEYPSGIRAGLEAMLLGRPTLC
                     TQSEIINEYTAPSEQEIVYCAPHSVDDMARALSAIIALPEDERQKMCQRAQEKVLARY
                     SMAQFVAELEQVFHEMAAEAP"
     gene            2959..3867
                     /locus_tag="PKZ52_06015"
     CDS             2959..3867
                     /locus_tag="PKZ52_06015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNG59362.1"
                     /translation="MSLRVSVIIPAYNSARTIERALRSVVEQTLQPHEIILVDDGSSD
                     DTVALVRRTFPSVIIIAQANAGASAARNRGVAEATGELIAFLDSDDAWHLQKLQMQVG
                     VFERSPQLNFLSTQCEYFTEQKWLAEQPLKLAHFDRVDIKELDFYQVFAHPFLATPSV
                     MMRKAFFDSLAGFDQALETAEDVDLWLRAAYHGRYALVSAPLTIVIAQEESLSTRTLH
                     SPYGQHLVVLERFCRGKQFSWTFWCWILPKTKSDIYCKWGSTLLVIQRPVEAWYKLTR
                     SLLCMPTARGFFLWLKALLQILAPKT"
     gene            4824..5723
                     /locus_tag="PKZ52_06020"
     CDS             4824..5723
                     /locus_tag="PKZ52_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59363.1"
                     /translation="MKCLKVLAAGLAVACWNNSALAWRLDLNFNNGEVGKAADRSPAV
                     FGSAGGVIYTTAKSFEGGKAVEMNISAGDTGFGVWGGIIGHPSLLKKGDQIWFRVRTY
                     MPSGFNYDSTGEGGHLKFLRIHTQSAGGENEGYNDWYINPRGSDNSHKYIFEGEQRWY
                     DMVPTSYQPQYDVWESYEFYVKLDDVPVSKGGQGRVRAWKNGKLLADITDRKTLVSAS
                     SVSDRTHMFTYWNGGSPKTQKMYVDDVVLTSDQPTHKDANGFYCVGTEETATGQLPAQ
                     GATGSAQPVMAPPQNLKATKSAL"
     gene            complement(5762..6736)
                     /locus_tag="PKZ52_06025"
     CDS             complement(5762..6736)
                     /locus_tag="PKZ52_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59364.1"
                     /translation="MSSQPSIVWIAWENHTRNRSLSAELHAHLVQLDYPHQNRLVRYA
                     QSISKTIALLWRWRSKIVIAQNPSIILCFLCVILRPLLRYRLVIDAHNAGIFPKEGRS
                     YWLQKIADMIIRQTAYTIVTNQGMAKIIEEKNGRPIVLPDPLPTLHTPPPAEPLAHSV
                     IFICSWAADEPFIEVFKAAEQLPELNFYVTGKSKGREQQFGRALPANIKLTGYLPELE
                     YLQLLNSCDIVLDLTTRDHCLVCGAYEAVSCKKPFVLSNNQATQDYFRLGGTYCDNTA
                     PSIASCLRKVYLNKENYIKEVETLHAQLQHEWHQSLHQAQRLIENKPL"
     gene            complement(6733..7929)
                     /locus_tag="PKZ52_06030"
     CDS             complement(6733..7929)
                     /locus_tag="PKZ52_06030"
                     /inference="COORDINATES: protein motif:HMM:NF040264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNG59365.1"
                     /translation="MICGYLFFEFVRPQGIFPAIDVLPYAQLLLLGSLAGAIADPTIR
                     WTSSPANKWLIFYFLWIQLSTLTAYNQDYATYHFMDFFGWFVIYFLMLNIINTERRFY
                     ITIMIYMLAAGKIAFGTAKSWVMRGFGFTSWGLMGPNGYFHNSGELAVLMVMLFPLSY
                     YLFSYFRTQVATWEKILFNILWIAPILTVLGASSRGSQIALAGQLVLMFRKSLFKIKP
                     LIGIAILVNALIFLLPQEQKDRFTTAGDDRTSQQRLLYWKHGFTMIQENPLLGVGYFN
                     FVPYYNDYYSYDLLYPHAQLPHNILVQIGTDAGYPALIAFLMMFFFCIFTPLRCKAVS
                     PVFRHAGYGLGMGVIGYFIAGQFVTITYYPFFWIHLAFISALYNINQKAKQDLNFSPL
                     KQPLKT"
     gene            complement(7997..>8486)
                     /locus_tag="PKZ52_06035"
     CDS             complement(7997..>8486)
                     /locus_tag="PKZ52_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59366.1"
                     /translation="YDRLSNINFQSVQRLIFICEGNICRSPVAELIAQNRGVRAASYG
                     LSCTDNHPADPRAVAFAKHIGLDLSSHRTRNIAAYIYEPGDLLVGMEPRHLPKIARAL
                     GPEAPTTLLGLFCRPPLTYLHDPFNSSPSFFTRCEENVVAATEGLLQKAIWAHEENRC
                     NS"
BASE COUNT         2128 a         1817 c         2211 g         2330 t
ORIGIN      
        1 gcatataata aaaactgcgg taagcgtatt caatcgcaaa caatacaggg cttttatggt
       61 attgttggca tttttcttga tttcggctgt tattcaggtt gcgggcatag ctagtattgg
      121 accgtttatc gcggccctca caagccctga tttgctcaga aataatgtgg ttatgaaatc
      181 cttaatggct atgttaaatc tgcagaccaa tgttcaggta atatcatttg tagcagtggt
      241 gtcgcttgcg ttgttggttg tttccaacgc ggtttctgtg gcggcccaat ggctactact
      301 tacctttagt atggaaatcg gtggctctgt gcagggacga ttattcgcaa gcctaatctc
      361 tcgggactat ttatttcata aaaccaatga ttattcggca tctattccta aaatttcaca
      421 agacacattg cgtttcctct acatggtatt gcagccaatt ttgcagttgt tttctaacgg
      481 ttttgttgtt gtcattattt tggggggtat agttgctcta aatcctacgg tggcatttgt
      541 cgccgggtct gtgttgtcct ttgcttatgc gggcacttat tattatttaa aaaaacgaac
      601 cgcaagggca ggggatttac taacccagaa gaccgcagct acccacaagg tgcttaatga
      661 ggcatttgtg ggggtaaagg agattaagct ggtaggtata gagaggcagt ttgttgacaa
      721 gtttaatcag gtaaatcatc agggaatgca ggcggccact tttctaagtt tggctgttga
      781 tataccgcgt tacgttattg aaactatcgc gctctcggcg atctttatca gtgctatttt
      841 cgcattaatg gcgggcatag agccctcgtc tattttgcca attctgagct tgtatgcggt
      901 ggccggttat aaattattgc caaccatgca gcaggtttac aaggcggttt cgtcgattag
      961 tgcccatggt tctgtagcgt tggatatcga gagggaaatt gcacaatgct cacccgagcc
     1021 cattgcaaca gagctgaaaa cgtcaaagaa ccggtctatt ggtttggcag tggaagttaa
     1081 aagtgtctct tatcaatacc ccaatacaga tcaatacgcc ttgcagggcg taagtgtgca
     1141 gttttgcttc ggtaaaatta atactattgc cggtcattca ggctcaggta aatccaccct
     1201 gttggatata gccctgggtt tgttaacacc cacaagcggg cagttattgg tgggcggcga
     1261 gcccatttct aatcaaacgg cgcgggcata tcaacagcgt ataggttttg tgccgcagca
     1321 tgtgtttatt cttaatgata gcgtggcgcg caacgtggca tttggtgaag ctatcgagca
     1381 ttttgatgcc gagcgcttaa cgaatagttt aaaactggct ggtgccatgg agtttgtcag
     1441 ccgtttgcct gagggggtta atacggtttt ggggcaagat ggcaaactgc tcagtggcgg
     1501 tcagcggcaa aaaattgcca ttgcccgctg tttatatcgc aattcggata ttttattttt
     1561 agacgagccc accagtgctt tggatataga cgctgaattt gaatttatgc aggtgttgga
     1621 agcgttaaaa gcgcgctacc tcattataat tatctcccac cgcccttcgg ctattcgtta
     1681 ttcagatttt atttgtctta tgaaggatgg tgttatcgag gcggcgggta gctttgatga
     1741 gcttaaacag tgtagcgatt cgtttcgaaa tttaattgaa aaaagctcga tggagtagcc
     1801 cctgatggcg ccaaataatt taaaaatagt gtattttttc aatcatccta ccgctgagga
     1861 tgagcgaaaa attgccagcg gcgagattcc ttcggaccgc ctctatgcca tggtggagct
     1921 taaacggcgc ggttggcagg tagatgcctg tgatgagcgc ttaagcgact ttgaatataa
     1981 aatgcggccg tggatgagct ggattaattg ggcctgtatc aaaaaggcga tgcaatttga
     2041 cgtctggctg gtgaaggata atttttcatt tattctcagt attctggcgc ggctgtttgg
     2101 caaaaaactg ctctatattg acagcttgtt tcattaccca aaaagccgat tgcggcgcgc
     2161 ttctatttgg ctgaatgtgc gcttggccca acagatcggt gtttattcgc attttcaggc
     2221 agatttttgg tcgcgtaaaa tgtcagtaag ccgcgccaag tttcgcgttt ttagttatac
     2281 cgtggaccat gagttttatc tgcaaggctt tgccaatgcg caggcgcaca ccgaagatga
     2341 ggcttttgtg ttggcgacag gccgagatat ggggcgcgat tttgctaccc taattgaggc
     2401 caccgcgcgc ctaggcatcg gcttgaagtt agtcaccttg ccgtatttat tgcccaaaaa
     2461 tatcgcagaa tatagccatg tgcagattct gcagcgcatc tcctacgctg agttatttgc
     2521 cctttatcgg gatgcagcgg tagtggcggt gccgctgcga gacaatattg aatacccgtc
     2581 gggaattcgt gctgggcttg aagctatgtt gttaggtcgt ccaacgctct gcacacaatc
     2641 ggaaattatc aacgaatata ccgccccttc agagcaggaa atagtttact gcgctcccca
     2701 ctcggtggat gatatggcgc gcgcattaag cgcgattatt gcgttgcctg aagatgaacg
     2761 gcaaaaaatg tgtcagcgtg cgcaggagaa agtgctggcg cgctattcga tggcgcagtt
     2821 tgttgccgaa ttagagcagg tatttcatga gatggctgca gaggcgcctt aacctgttaa
     2881 accgaccaaa cgaccccaaa gtatgcgatc tgagtgtttc ttaataatga ttctggttat
     2941 gtggatggca aggccgttat gtctttgagg gtcagtgtaa ttattcccgc ctacaactcg
     3001 gcgcgcacga ttgagcgcgc gctgcggtcg gtggttgagc aaacactgca gccccatgaa
     3061 attattcttg ttgatgacgg ctcaagtgat gataccgttg ccctagtgcg gcgcacattt
     3121 cccagtgtaa ttataattgc ccaagctaac gcaggtgcat cggctgcgcg caatcgcggg
     3181 gtggcagagg ctacggggga gttgattgcg tttttagatt ctgacgacgc ctggcaccta
     3241 caaaaactgc agatgcaagt gggggttttt gagcgctcac cgcaattgaa ttttttatcg
     3301 acacagtgtg aatattttac cgagcaaaaa tggctggctg agcagccgct aaagttagcg
     3361 cactttgacc gcgtcgatat taaagaattg gatttttacc aggtatttgc gcatccgttt
     3421 ttagccaccc cctcggtcat gatgcgcaaa gctttttttg attcattggc ggggtttgat
     3481 caagctttgg aaaccgctga agatgtggat ttgtggctgc gagcggccta tcacgggcgc
     3541 tatgctttag tgtctgcgcc gctgactatc gtgatagcac aggaagaatc tttatccaca
     3601 cgcacattgc attcacccta tgggcaacac ctggttgtgt tagagcgctt ttgtcgcggt
     3661 aaacaattca gctggacatt ttggtgttgg atattaccaa aaacaaaaag tgacatttat
     3721 tgtaaatggg gctccacttt gttggtcatc caacggcccg tggaagcctg gtataagctg
     3781 acacgctcat tgctttgtat gcctacggcg agaggttttt tcctctggct taaagctttg
     3841 ctacaaattt tggcgccaaa aacctaatcc taagtgtaag gcgcctctga taatttaaat
     3901 ttcgctaagg tcgcactgta ttttcgtgtg ggcgactggg gtttagagtt tcggtaacta
     3961 ttcagcatgt ttttatgtgg ctggaggtaa ttcccgtact cgctgggaac tatcacgggc
     4021 gtgttttaat gctgtaaccc cgcaatactc ccccgccgtg gtgactatag gttagtgcag
     4081 acacgccgtg aatacatccg tgtaggctcg tcaaaaaggt ccctcttttt gacggtctgc
     4141 actaacctat agtcacccca gctacaccgc gaatcgatta tgccgcatgg ctaaatcctc
     4201 cagaatttgc cgcctgcgcg gaagagggca atatagtgtg ctgaatagtt aagagtttcg
     4261 agtgatgcag gcattcgagt gacgcaggac gccaaaaccg aggaagacaa aaccatatgg
     4321 gtgagtgaaa cccgccaggc caagctgggt tagcggggct ataggttcat agcactagtg
     4381 aactatttgc cctttttata cttgaccatt gattatattt ttttgtactt aaagaattta
     4441 ctttgcgctg gtagcgaata atcatcagta aattattgca atgcatattg gcaaaaatat
     4501 aggcgtcatt ttgataccgc tattaggttc cattgccttc agtaatttat aaccttaggt
     4561 tggtgttttg tattaattta gttttattaa gtctaaaaat atacgcccat caatgtttgc
     4621 atgtgcatag aatgccgccc gctttgggtt atccgtcatg ccatgtttat ccgtgccggg
     4681 caaatatcga ggaataaact gatggttttg attgttgggt cacatttgca atgagcgaga
     4741 attcgtgata attgaactat ttggtgcgca ggccatagcc tgggctatgg ttaccagtag
     4801 gtacacacaa tgaggtttcg ggtatgaaat gtttgaaggt tcttgcggca ggcctggctg
     4861 tagcttgttg gaataattcg gcgctggctt ggcgtttgga cttaaatttt aacaacggtg
     4921 aggtgggtaa agcagctgat cgctctccgg ccgtgttcgg ctccgctggt ggagtgattt
     4981 acaccaccgc caagtcgttt gaaggtggca aagcggttga gatgaacata agcgctggtg
     5041 ataccggctt tggtgtgtgg ggcggcatca tcggccatcc cagcctgttg aaaaaaggcg
     5101 atcagatctg gttccgtgtg cgtacctata tgccctcagg ttttaattat gactccacag
     5161 gcgagggcgg ccaccttaag ttcctcagaa tacataccca gtccgccggt ggcgagaatg
     5221 agggctataa cgactggtat atcaatccgc ggggcagtga taactcacac aagtatatct
     5281 ttgaaggtga gcagcgctgg tacgacatgg tgccaacctc ctatcaaccc cagtacgacg
     5341 tttgggaaag ttatgagttt tacgtcaagc tggacgatgt accggtttcc aaaggtggcc
     5401 agggccgtgt gcgcgcttgg aaaaacggca agttattagc cgatattacc gatcgtaaaa
     5461 ccctggtctc agccagttct gtgtcggatc gcacccacat gttcacctat tggaacggcg
     5521 gctcgcctaa aacccagaaa atgtatgtag atgatgtggt gctgacctct gatcagccta
     5581 cccataaaga tgccaatggc ttttattgtg tgggtacgga agagacagca actggccagc
     5641 tacccgctca aggtgccact ggttcggctc agccagttat ggccccgccg caaaatctta
     5701 aagccaccaa atctgcgctc taattaataa ccctttaacc cagtggcgcc caggagcggc
     5761 gctacagggg tttgttctct attaggcgtt gcgcctgatg cagagactga tgccactcgt
     5821 gttggagctg cgcgtgcagt gtctcaacct cttttatgta attctcttta tttaaataga
     5881 ctttgcgcag gcagctggca atgctggggg cggtattgtc gcagtaggtg ccgccgagcc
     5941 gaaaataatc ctgtgtcgcc tggttattgg ataatacaaa cggcttttta cagctcaccg
     6001 cttcataggc gccgcacacc aggcagtggt cgcgggtggt gaggtccagc actatgtcgc
     6061 aggaatttaa cagttgcaga tactctaact ccggtaaata accggtcaat ttaatgttgg
     6121 cgggtagcgc ccggccgaat tgttgctccc gccctttgct tttacctgtg acataaaaat
     6181 ttaattctgg cagttgttct gccgctttaa acacttctat aaagggttca tctgccgccc
     6241 aggagcagat aaaaataacc gaatgtgcca aaggctcagc cgggggaggt gtgtgcagtg
     6301 tgggcagtgg gtccggcagc acaatagggc ggccattttt ttcttcaata atttttgcca
     6361 tgccttgatt ggtaactatg gtgtaggccg tttggcgaat aatcatgtcg gcaatttttt
     6421 gcagccaata actccggcct tctttcggga agatacccgc gttgtgagca tcaattacca
     6481 aacggtagcg caataaaggg cgcagaataa cgcataaaaa acacaatatg atggaggggt
     6541 tttgcgcaat aacaatttta ctgcgccagc gccacagtag cgcgatggtt ttgctaatgg
     6601 attgcgcgta gcgcaccaaa cggttttggt gcggataatc caattgcact aagtgcgcat
     6661 gtaattccgc acttaaggag cggttgcggg tgtgattttc ccacgcgatc cagacaatag
     6721 agggttggct gctcatgttt ttagtggctg ttttaatggc gagaagttta aatcctgctt
     6781 ggctttctgg ttgatattgt aaagggccga tatgaaagca aggtggatcc agaagaatgg
     6841 ataataagta atggtgacaa attggcccgc aataaaatag cctattacgc ccatgccaag
     6901 cccatagcct gcatgtcgaa agacaggcga aacggctttg catctaagag gggtaaatat
     6961 acaaaagaaa aacatcatta aaaatgctat gagcgctggg taaccggcat ctgtgcctat
     7021 ctgcaccaat atattgtggg gcaattgagc gtgtgggtag agcaggtcat aggaataata
     7081 gtcattataa taggggacaa agttgaaata accgacgcct aggagggggt tttcttggat
     7141 catggtgaag ccatgtttcc agtagagcag cctttgttgg gaggtgcgat catcgcctgc
     7201 ggtggtgaag cggtcttttt gttcttgggg cagtaaaaaa ataagtgcgt tgactaaaat
     7261 agcaatacca attaatggtt tgatcttaaa aaggcttttt ctgaacatca acaccagctg
     7321 gccagccagc gcaatttgtg agccacggct gctggcgcct aacacggtaa gaatgggggc
     7381 aatccaaaga atattgaata agattttttc ccaggtggca acctgtgtgc ggaagtaact
     7441 aaagaggtaa taggagaggg ggaacagcat taccatcagt acggccagtt cgccggaatt
     7501 atgaaagtaa ccattcggcc ccataagtcc ccagctggta aaaccaaacc cgcgcatcac
     7561 ccatgatttt gcggtgccga aggcaatttt gccggccgct agcatataaa tcatgatcgt
     7621 aatataaaag cgccgctcgg tattaataat gttgagcatc aaaaaataaa tgacaaacca
     7681 gccgaaaaaa tccatgaagt ggtaggtggc gtagtcctga ttgtaggccg tcagtgtaga
     7741 cagctgaatc cataaaaaat aaaaaattaa ccacttgttg gcgggggacg acgtccagcg
     7801 tatggtcggg tctgcaatgg cgcctgccaa gctgccgagc agtaacagtt gcgcgtaggg
     7861 taaaacatct attgccggga aaatgccctg ggggcgcaca aactcaaaaa acagatagcc
     7921 gcaaatcatc caaaatgaat agtgctcctg cttgatgtag gcccatattt ttttaaattt
     7981 aaaggcgtag aactcgtcaa gagttacatc gattttcttc atgagcccaa attgcctttt
     8041 gcaataaacc ttcagttgct gccaccacat tttcttcgca gcgcgtaaaa aagctagggc
     8101 tactgttaaa tgggtcgtgt aaatacgtca gcggtgggcg gcagaataag ccaagtaggg
     8161 tagttggcgc ctctgggccg agtgcgcgtg caattttagg taaatggcgc ggctccatgc
     8221 ccactaataa atcgccaggt tcataaatat aggcggctat gttgcgcgta cggtggctcg
     8281 ataaatcgag cccaatgtgc ttggcaaatg ccactgcgcg ggggtctgcg ggatggttgt
     8341 cagtgcaact aaggccatag gaggcggcgc gcaccccgcg gttttgcgca atgagttctg
     8401 caacggggct gcggcaaatg ttgccttcac aaataaaaat tagccgctgc accgattgaa
     8461 aattgatgtt ggatagacgg tcgtaa
//
LOCUS       DAOQGS010000115         8483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3964_length_8483_cov_4.201709, whole genome shotgun sequence.
ACCESSION   DAOQGS010000115 DAOQGS010000000
VERSION     DAOQGS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_8483_cov_4.201709"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKZ52_06040"
     CDS             complement(<1..554)
                     /locus_tag="PKZ52_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59367.1"
                     /translation="MTNKDFFFGDVLNIYNSGALDLGSVNGLVRKVSPEYFTSQLNRL
                     LRSNGGKIAVTDSRSCPDFAAFLRGIHKDAIGLIEIYARNDVNTCVEATLACDVFLQD
                     GVVSVVAHWCGRNAERQREIVNTLLKPIHAAGLDKKLYLRFDNKVERALCNEDYEEEV
                     KRVIRLSKTAPDIDTETTAIELAEV"
     gene            complement(814..2793)
                     /locus_tag="PKZ52_06045"
     CDS             complement(814..2793)
                     /locus_tag="PKZ52_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59368.1"
                     /translation="MNKLVTYILCGIISFGLVGCGKGGGKEGSKESSSVTITEKGEST
                     ISVKEGDSVAIFLELDSFSGAKASRLEARKINKSDVIHEDPNPSTGVFANGDVQYIKF
                     IKIKGPDIESEKTIAPYSKNKQFGISFIAPDTKGAATVEYEYKIIMVNDKGASDESVR
                     KITVLGVNNPPVIKLAPTAQVSPNRSVSLYATETTDIDGSVASYSWVQISGTNVTLAN
                     TNQATLNFIAPNSVQAEQLVFQLTATDNLGATSTQKVTVTVVPLDPPTVSIRFPSATS
                     YTTANYIRVSGYASAKNGATIKSIRVQVDNDTVITTMNGNSWSVSGFDLPTAFDSVPV
                     VATVEDSQGKISEARATIQRKAPIVSDLKWNKLIGLDINPLNGGLLALINDNSLFDDY
                     ALATFSFDSKSTDKLLYEFPNAAFESVPRFDGTVATAFNVLDNKYYVSSTVQSVGKST
                     SLIHSVDLTAKKMTQIYPLDINKDVSLASITGIAVDRNADVLVVDGVQSKLYSIDPDK
                     FEFKVQAEGLFPVDKVNFISLATDPFAEKFSPYLLPNKNDMPLYGLNKSTKLVEQIAG
                     DFSGGTTAAKRFVVDEVLNTAYVLDTKGDVYRLNLKNNTRGLFAKGPIGDIAYDPVDS
                     ILYFYYTDSKSIHMIDVFSGESVEITRSKSDVLLK"
     gene            complement(2924..3469)
                     /locus_tag="PKZ52_06050"
     CDS             complement(2924..3469)
                     /locus_tag="PKZ52_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59369.1"
                     /translation="MKNRLSLIVVALLMFGCASTSVRPENKLSANAKFLEPSLKVNLM
                     QKLTVEGYPDQSEFSKLLQQKTIEALKAKNLIAEKSSEGALLVEIVVDYQRRFAGEDT
                     PIPSKSVMMPIVHYRIVVSGNGIEKRIIDKDGMTVNRGFGSNLATTFTLGLGKTAKDE
                     LKDLEMLANGIASEVEALKER"
     gene            complement(3573..3791)
                     /locus_tag="PKZ52_06055"
     CDS             complement(3573..3791)
                     /locus_tag="PKZ52_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59370.1"
                     /translation="MNKNEVAAQLVYLLNKYHGDESEKRSLIAIINDPSRPGLPVRGC
                     LEDMRPYIKQGLSDADKLVIDNLLCCFG"
     gene            complement(3885..4106)
                     /locus_tag="PKZ52_06060"
     CDS             complement(3885..4106)
                     /locus_tag="PKZ52_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59371.1"
                     /translation="MRIEVLNDKNELKKILSRVVLAADEKAELLAVVDMHPSSIPLKA
                     VLEMLQNVNTLPLSVEETDILDGLKFIYI"
     gene            complement(4277..4696)
                     /locus_tag="PKZ52_06065"
     CDS             complement(4277..4696)
                     /locus_tag="PKZ52_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59372.1"
                     /translation="MKIIENCWDQIETYNCQYGAHIFEGSIAKIYVNHWLDVAPEMVR
                     LFSRRNDEGFVGHCILAFFGVKNFKFSVRQWGMQGEKTVWREPVDFCYEGVEAGRLIS
                     YEFEGSLHGFPSSVFVAIEAERFELHVLDNDEPARQS"
     gene            complement(4700..>8483)
                     /locus_tag="PKZ52_06070"
     CDS             complement(4700..>8483)
                     /locus_tag="PKZ52_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HNG59373.1"
                     /translation="LTTGSKNADGSLNTQNTQLDMQGAEISARDISINTGTLNASSRE
                     DTERSSSENKQGQISAQVTVYGAPGGASVNGSYQRAESDSQSTTVHNTRIHADTLSIS
                     TSGDANLKGATARADEKLTVNVDGNLILDSQQNRSSSKSHEQGMSAGVGLTTGGEANS
                     TNAGVSQGNGRSRTQETVVTSLTSGGTAEITVKGNTHLGGAIIGTLEQVDVCADQNSK
                     ACKNEQVFGKDLNQLTFTTGSLTFNDLLNLDTSTSSSFGLSTNLSLGNGQDAQPANPR
                     VGQNNAKTATGDRLSPTSSTISLANSNHNTATKTLSTLGQGNITVGGVELEKDGKLTA
                     AGVTATQKGSLKGLNRDTEKTEKALWDSHQEMKVDASVDHRLFTEEGHKQIKNDAVDT
                     AEFGGDIAAATQQVVEQEDLGALDFWHTLDDNTKATQVKHDLLRNPEYSWILEGLKSD
                     KNGDAYAQALQGITTLAQQKYGMSADELSSLYLYNAKETSSVSLGDTSIADVKGGIVL
                     DAAHSEFGNLFLDVSDGSAKTDHLISLGHEVYESTTLFGGGVNDDKQELFADNFGNRL
                     STRLDQAAGGNLDTTGGRSFNTSLLNSQSVQLGTLHANTVGNTLVDHRQLGGKELTAI
                     KNMAPDYAKKKGVSLEQARKELTQQALRMVDKTWSEQAHIEENTDARNFLLTNSEGQS
                     FVIGGHLGGLVGGETTSLFTSNEEQFNDKYLLANEATDNELGKFRAPQSPYEVTPLML
                     SDYDLRGWLERYATADGTRPVELKDVSAGDYAEQVGKDSVVAGTFLGKQVTKTQPEIA
                     IDLLEGAYKLAGKAMGACQQEGASCLLPSTDTHGSAGDKANIDSLTGNKDAFIRNSTF
                     AGYSTAFDLANVALMAYPFGAGAKAVEVSAGELLLVNETKVASNKLGDLSPGVLAEGD
                     FGYLNQRAKNLVEDATNSAELRGSPDFYVGPAGPESTLQGTGYRYMRLYNDDGTVNKY
                     ALVTLDTNAAPVTYFGFEKFESGAAARDAFQIKGPELGDSWSDARLVGTFDTLQIFDA
                     KGTPTVRHPYFAGDDISTGKIEPFTEAYPQYGKGGVEQLTADRQEIKFNKLDILPAEL
                     KPDTKAAIPTLLNLRGDTALGGIRDVRVGETVELFTDARGVQIPGAKGVGPNDFSAVA
                     IDARNMPNIASNSQVKVVASNPYIPKSAGGNFTMMDYLPEATRITQPGGEIVINGTAA
                     NKYLTNLPTQAQLDVLGLEVKYQGTLLPEYQGQTYLRADGSKLTEPMQSIVFRKKK"
BASE COUNT         2171 a         2143 c         1729 g         2440 t
ORIGIN      
        1 acctccgcca gttcaatggc tgtagtttca gtatcgatat cgggagcggt cttggataag
       61 cgtattaccc gttttacctc ttcctcataa tcttcattgc agagagctct ttcaactttg
      121 ttatcaaaac ggagatataa ttttttatct aatccggccg catgaatagg cttcagcaat
      181 gtgttgacaa tttccctttg cctttctgca tttctgccgc accaatgcgc gacaacactt
      241 acaaccccat cctgcagaaa tacatcacag gctaaagttg cttcgacgca ggtattgaca
      301 tcgttccttg cgtagatttc tatcagaccg attgcatctt tatggatacc ccttagaaat
      361 gctgcgaaat cagggcaact tctactatcg gtaaccgcta tttttccgcc attactgcgc
      421 agcaacctat ttagctggga cgtaaaatat tccggtgaaa ccttgcgcac caatccattc
      481 acgcttccaa ggtccaatgc accactgtta tagatattta acacgtcacc gaaaaagaaa
      541 tctttatttg tcatttgggc tcctatactg caggtgatga tttaatgggg ctcattgaga
      601 tcgcgaatcc agacgcactc atcacaaaca ttcaaacacc actgaaccgg aaaaaatcga
      661 accggatctg cggtctaaac gtattgacag ctccagctgc actgagatga aaaaagatca
      721 tatttcccaa tcaatttcca ccccaagtgt atctgcgata aatataaaaa tgccgctgct
      781 tttgagctcc ttaagctgcg gcatttggtt cttctatttt aacagcacgt ctgacttcga
      841 ccgggtgatc tcaacagatt caccggagaa aacatcaatc atatgaattg attttgaatc
      901 ggtgtagtaa aaatacaaaa tactatcaac ggggtcataa gcaatgtcgc caataggccc
      961 cttagcaaat aacccacgag tgttattctt aagattcaat cggtacacat ctccttttgt
     1021 atcaagcaca taggctgtat tgagaacttc atcgaccaca aaccgttttg ccgccgtggt
     1081 tccacccgaa aaatcacctg cgatctgctc caccaatttt gtgcttttgt tcaatccgta
     1141 tagcggcata tcatttttat tcggcagaag atagggggaa aatttttccg caaaagggtc
     1201 cgttgccaaa gaaatgaagt tcaccttatc cacggggaat aatccttcag cttgtacttt
     1261 aaattcaaac ttatcagggt cgattgaata gagcttgctc tgaacgccat cgactactaa
     1321 cacgtctgca tttcgatcaa cggcgatacc cgttatcgac gccaacgaca catccttgtt
     1381 tatatccagg gggtaaatct gtgtcatctt cttagcagtt agatccaccg aatgaatcaa
     1441 cgaagtcgat tttccaacag attggactgt agagctcacg taatatttgt tgtccagcac
     1501 attgaatgcg gtggcgacag taccgtcgaa tcttggcaca gactcaaagg ccgcgtttgg
     1561 aaattcataa agaagtttgt ccgtcgactt tgaatcaaag ctaaaggtgg ccagtgcgta
     1621 atcatcaaac aaactattgt cattaatgag ggctagcaac ccaccattta atggattgat
     1681 atccaagcca attaatttat tccatttaag atcgctcaca atgggtgctt ttcgttgaat
     1741 tgtggcgcga gcttctgaaa tcttgccctg agaatcttca accgttgcca cgaccggaac
     1801 ggaatcgaat gctgtaggca aatcaaagcc agacactgac caagagttac cattcatcgt
     1861 cgtgataaca gtatcattgt caacctgaac ccttatggac tttattgtgg caccgttctt
     1921 ggctgaagca tatccggaca cccttatgta attggccgtt gtgtaggatg ttgcagacgg
     1981 gaatcggata gacactgtag gaggatccag cgggacaacc gtgacggtga ctttttgcgt
     2041 gctggtagcg ccgagattgt cagttgcagt taactgaaat accaattgtt cagcctggac
     2101 agaattgggc gctatgaaat tgagcgtggc ctggttggta ttggcgagcg tcacattcgt
     2161 accgctaatt tgcacccagc tataggacgc tacgcttcca tcgatatctg tcgtttcagt
     2221 ggcatacaga gataccgacc ggttgggtga cacttgggcg gtcggtgcga gctttatcac
     2281 cggcgggtta ttaacaccga ggactgtaat tttacgcaca gactcgtcag aggccccctt
     2341 atcgttgacc attataattt tatattcata ttccacagtc gcagcgcctt tggtatcagg
     2401 cgcaataaaa gaaatcccaa actgcttatt cttactgtaa ggcgcaatgg ttttttccga
     2461 ttcaatatcg gggcctttta ttttaataaa tttaatgtac tgaacatcgc catttgcaaa
     2521 cacgccagta ctaggatttg gatcttcatg aatgacatcg gatttattga tttttcttgc
     2581 ctcgagtctg gatgccttag cgcctgaaaa gctatcaagc tccagaaaaa ttgccaccga
     2641 atcgccttct ttaactgata tagtgctctc acctttttca gttatggtca cagacgaaga
     2701 ttcttttgag ccttcttttc cacccccttt cccgcaacca accaaaccaa aagaaattat
     2761 cccgcacaaa atatatgtta ctagcttatt cattaaccgt attccattca aaaaatttaa
     2821 caagtagcag acagttgcca cccttttgat taaaaataaa aacctctgtt atttgccttt
     2881 caaccaacca accaacgtct gtcagcgaac caaaatcgaa agactaccgc tcctttaatg
     2941 cttcaacttc acttgctata ccattcgcaa gcatttcaag atccttaagc tcatccttcg
     3001 cagttttacc caaccccagg gtaaatgttg tagccagatt actgccaaac ccacggttta
     3061 cagtcattcc atccttatca attattcgct tttcaatacc attgccactc acaactatcc
     3121 gataatgtac gataggcatc attacagatt ttgaaggaat tggcgtatcc tctcctgcaa
     3181 atcggcgctg atagtcaacc acgatttcaa ctaaaagagc gccctcactt gatttttccg
     3241 caatcaaatt tttcgcctta agtgcttcta ttgtcttttg ctgcaacagc ttagaaaact
     3301 cagattgatc aggataaccc tccactgtaa gtttctgcat tagattgact tttaacgaag
     3361 gctccaaaaa ttttgcattc gctgatagct tattttcagg cctaacagag gtgctcgcac
     3421 aaccaaacat caacaatgca accacaatca atgacagacg atttttcatt tttctattcc
     3481 caataaaata ttttaaaaat agcctaagtt tacccaatat agtgcaacgc aaatatatta
     3541 ccatccaatc acgtgttagt cgggtcattt cattaaccga agcagcaaag caaattatca
     3601 atcaccaatt tatcagcatc agacaatccc tgcttaatat acggcctcat atcctccaaa
     3661 catcctcgaa caggaaggcc tggcctactt ggatcattaa taattgcaat caaacttctc
     3721 ttttcacttt catccccatg gtatttatta agcaaataaa ccaattgagc tgctacctca
     3781 ttcttgttca tcaggttttt tctcgggtaa ttatggctgt gacgcctacc acttccattg
     3841 gcgccaactg ctgcacacca cctttaccat attcagggca acagtcaaat ataaataaac
     3901 tttaagccat cgagaatatc agtctcttcc acactaaggg gaagggtgtt cacattctgc
     3961 aacatttcga gcacggcctt caacggaata ctagatggat gcatatccac aacagcaagt
     4021 agctcagcct tttcatcagc agccaatacc accctagata atattttttt caattcattt
     4081 ttatcattca gaacttcaat tcgcataaaa aacaccagaa aattataaaa ccaaccattc
     4141 agcctacatt ccacatcaca ccaaaccagt ttccgcttaa aattaagccc aaccccgaac
     4201 taattcaaca tttttaaccg cgtcattgcc acataaaaga ccaggaactc agccactttt
     4261 caaccccaca aaaaaatcaa gattggcgcg caggttcatc attatccaac acgtgaagct
     4321 cgaatcgttc agcctcaatt gccacaaaaa cagaggacgg aaacccgtgc aaactacctt
     4381 caaattcgta tgaaattaat ctccccgctt ccacaccctc ataacaaaaa tcaacaggct
     4441 cacgccagac tgttttctct ccctgcatcc cccattgccg aacactgaat ttaaaatttt
     4501 taactccaaa aaaagccaaa atacaatgcc caacaaaacc ttcatcattt ctccgggaaa
     4561 acaaccttac catttccggc gcaacatcta accagtggtt tacataaatt tttgcaattg
     4621 agccttcaaa aatatgtgct ccatactggc agttataagt ctcaatttga tcccaacaat
     4681 tttctattat cttcataaat cacttctttt ttctgaatac gattgactgc ataggttcgg
     4741 tcaattttga cccgtcggcg cgcagataag tttgcccctg gtactcaggc aacaaagttc
     4801 cctgatactt gacttccaac cccagaacat caagttgtgc ctgcgtcggc aagttagtca
     4861 gatacttgtt tgccgctgtt ccattgataa ctatctctcc tcctggctga gtaatccgag
     4921 tggcctcagg taaatagtcc atcatggtaa aattaccacc cgcacttttt gggatatatg
     4981 ggttgctcgc gactaccttg acctgactat tgctagcaat attaggcatg ttacgagcat
     5041 caatcgccac agcgctaaag tcattcggtc caacaccttt ggcccccgga atttgaaccc
     5101 ccctagcatc agtaaataac tcaactgtct caccaactct gacatccctg ataccaccta
     5161 aagcagtatc accgcgaaga tttaataaag ttggtatagc tgccttggtg tccggtttta
     5221 attcagcggg caatatatcc aacttattaa atttaatttc ctgccggtcg gccgtaagtt
     5281 gctctactcc acctttgcca tattggggat aggcttctgt aaatggctca atttttccag
     5341 tcgatatatc atcaccagca aaatatggat gcctaactgt tggggttcct tttgcatcaa
     5401 atatttgaag ggtatcaaag gttcctacca gccttgcatc actccaagaa tcccctaatt
     5461 cggggccttt tatttgaaac gcatcgcgag ctgcagcacc gctttcaaat ttttcaaatc
     5521 cgaagtacgt gactggtgca gcattcgtat ctagagtgac aagggcgtat ttattcacag
     5581 ttccgtcatc gttatatagc cgcatataac gataacctgt tccctgcaag gtggattccg
     5641 gtccagccgg acccacgtaa aaatcgggac ttccccgtaa ttcagctgag tttgtcgcat
     5701 cttcaacaag gtttttggcg cgttgattta aatacccaaa atctccctca gccaacaccc
     5761 ctgggcttaa atcccctaac ttgttcgacg cgaccttagt ttcgttaaca agcaataact
     5821 cccccgcaga tacttcaacc gctttagctc ccgccccgaa tgggtaagcc atcagggcta
     5881 cgttggcaag gtcaaaagcg gtgctgtatc cggcaaaggt gctattgcga ataaacgcgt
     5941 ctttattgcc tgtaaggcta tcgatattgg ctttatcacc ggcggagcca tgggtatccg
     6001 tcgagggcaa cagacagctg gcaccctctt gttgacatgc ccccatagcc ttaccggcca
     6061 gtttgtaggc cccctccagc agatctattg caatctctgg ttgagttttc gtcacttgtt
     6121 tacctaaaaa cgttccggcg accacactat ccttaccgac ctgttctgcg taatctccgg
     6181 cgctcacgtc tttgagttca actggacgag tgccgtctgc agttgcataa cgctccagcc
     6241 aaccccttaa atcgtaatcc gatagcatta atggcgtaac ttcgtagggg gactgtggcg
     6301 ctctgaattt gcctaactcg ttatcggtag cctcattggc aagcagatat ttatcgttaa
     6361 actgttcctc atttgaagta aataacgatg tggtttcgcc tccaacgagt ccgcctaaat
     6421 gaccgccaat tacaaaactt tggccctctg aattagtgag taaaaaattt cgcgcatcag
     6481 tattttcctc aatatgcgct tgttcactcc aggttttatc caccatgcgt agagcttgtt
     6541 gagtgagttc cttgcgcgct tgttctaatg aaacaccttt tttcttagcg tagtcgggtg
     6601 ccatattttt gatggcggtc aactctttac cgcctagctg acggtgatct accagtgtat
     6661 tgccgactgt gttggcgtgc agtgttccta attgcaccga ctgactgttg agcaaagaag
     6721 tattaaaact tcgcccgcca gtggtatcca aattgccgcc agccgcttga tccaatcgag
     6781 tcgagagacg gttgccaaaa ttatcggcaa acagttcctg tttatcatca ttgacaccgc
     6841 caccaaagag ggtggtgctt tcatacactt catggccaag ggaaattaaa tggtccgttt
     6901 tggcactacc atcactcaca tctaaaaata aatttccgaa ctcgctgtgg gcagcatcta
     6961 aaacaatgcc gcctttaacg tcggcaattg aagtgtctcc caaggagaca ctagaggttt
     7021 cttttgcgtt gtataggtac aacgagctga gctcgtcggc actcatgccg tatttttgtt
     7081 gtgccaaggt ggtgattccc tgtagtgctt gcgcgtaggc atcaccattt ttatcggatt
     7141 ttaggccctc tagaatccag ctgtattctg ggttgcgcag cagatcgtgt ttaacctggg
     7201 tagcttttgt gttgtcatcc agggtatgcc aaaaatccaa tgcacctaag tcctcttgtt
     7261 caaccacttg ttgagtagcc gccgctatgt cacccccgaa ctccgccgta tcaaccgcat
     7321 catttttaat ttgcttatgg ccttcctccg taaataaccg gtgatcaacg ctcgcatcca
     7381 ccttcatttc ctggtggcta tcccacagag ctttttctgt tttttcggta tcgcggttga
     7441 ggccttttaa tgatcctttt tgagttgccg ttacacccgc agcggtcaat ttgccgtctt
     7501 tttctaactc cactccgccc actgtgatat tgccctggcc cagagtgctg agggttttgg
     7561 tggcggtgtt gtggttgctg ttggcgaggg agatggtgct gctggtggga cttagcctgt
     7621 caccggtcgc cgtcttagca ttgttttggc caacgcgggg attagcgggc tgcgcatctt
     7681 ggccgttgcc taggcttagg tttgtgctga ggccaaaact gctactggtg ctggtgtcca
     7741 ggtttaacag gtcgttaaaa gtgaggctgc cggtggtgaa ggtgagttgg ttgaggtctt
     7801 taccgaatac ctgctcgttt ttacaggctt tgctgttttg atcggcacat acgtccacct
     7861 gctcaagggt gccgatgatt gcgccgccta agtgggtgtt gcctttgacg gtaatctcag
     7921 cggtgccacc gctggtgagg ctggtgacga cggtttcctg ggtgcggctg cggccattgc
     7981 cctggctgac tccggcgttg gtgctgttgg cctcgcctcc cgttgttaac ccaaccccag
     8041 cactcatacc ctgctcatgg cttttactgc tgctgcggtt ttgttgggag tctaggatga
     8101 gattgccatc tacattcacc gtgagttttt cgtcagctcg ggctgtagcg cctttcaaat
     8161 tggcgtcgcc gctggtggag atgctgaggg tgtcggcatg gatgcgggtg ttgtgcacgg
     8221 tggtgctttg gctgtcggac tccgcacgtt ggtaactgcc gttgacactg gcgccgccgg
     8281 gagcgccgta tacggtgacc tgggcactga tttgaccctg tttattttcg ctactgctgc
     8341 gctcggtatc ttctcggctg ctggcgttga gggtgccggt gttgatgctg atgtctcgag
     8401 cgcttatctc ggcgccctgc atatccagct gggtgttttg agtgttcaga ctgccgtcgg
     8461 catttttact gccggtggtg agg
//
LOCUS       DAOQGS010000116         8463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3979_length_8463_cov_5.178283, whole genome shotgun sequence.
ACCESSION   DAOQGS010000116 DAOQGS010000000
VERSION     DAOQGS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_8463_cov_5.178283"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..793)
                     /locus_tag="PKZ52_06075"
     CDS             complement(41..793)
                     /locus_tag="PKZ52_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59374.1"
                     /translation="MNMQRGATMISLLVGMVVSMLAILASLSMFHNLVRTSTEAKADA
                     RMEGDISLGVLRLDQELLAAGFNVDRAPGAGRNQDFALANNLVGNTPTSRVVWRLNDG
                     GNWVCRRAVSTFNIPQLQYTLELFEANAGLCLPGPAALPDLNDDTKWTRSEQLVSIRL
                     QALDAGTPVTLTRPLIEFSSDAFNDRACMPFGAAAPLAPGAAAPMHPNLRIHVFDTAA
                     IYAPAPNAANAAGPPRPDLARTHTLCLVNITL"
     gene            complement(796..1239)
                     /locus_tag="PKZ52_06080"
     CDS             complement(796..1239)
                     /locus_tag="PKZ52_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59375.1"
                     /translation="MYSLKQQSGDFLIEAMIGTLITALVSLGTLAITSRVMLTQEEMV
                     RQEFVVATLREELYTRDPFSAATVRCNNPNNNNKVTAARRLDFTQRGVNIAANATDGC
                     TPLTEVYVHPVTNTPYNARIESANLNIVVPTATDPAEYELGVDPQ"
     gene            complement(1241..1849)
                     /locus_tag="PKZ52_06085"
     CDS             complement(1241..1849)
                     /locus_tag="PKZ52_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59376.1"
                     /translation="MKRTLGFSLVELLVVLAVVAFLAFVTVPLASGWIRNAQLEKDFA
                     GLQRALGTAKALAMRNAKGVQAPDNFLPNTPLPSDNIAAVLCRKGDNLFLASAADLPA
                     ANRSCADANWNNISAGARPFYRMGQDVAITFGNQIAFCGAYFNARGRAKLCQGNPCTT
                     CTTSFDPATGLSVVVAGADTTQDPNNANRPDPNDQSNTIRAF"
     gene            complement(1864..2343)
                     /locus_tag="PKZ52_06090"
     CDS             complement(1864..2343)
                     /locus_tag="PKZ52_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59377.1"
                     /translation="MLKITCTQRGFSLIELLVAIAIVGILAAVAVPSYSNFIRRGEAD
                     AAYGDLQALSLAIEGRFRRVLSYPPTPATNNTAAVKDLVSSWGPSTKAAAAFNFQYTL
                     VNQGTATYRISAVGQTTSNFNNCTLVLEVVRGAATRFCNNPAVCKVKCDALPAQPAN"
     gene            complement(3173..5497)
                     /locus_tag="PKZ52_06095"
     CDS             complement(3173..5497)
                     /locus_tag="PKZ52_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59378.1"
                     /translation="MHTQPLSAQRGAATIIMSVLVGFSITAMGLALMFNVQNAQDKQI
                     TAQAQVSAQNLAWAGSEAFRQLLTTMPIGLVNVLPLNEPMTAISSANVTPAAGVPALA
                     GTITPTVTNRVLVAASGGLPARTEVTVNLAAVNSTAGVGTTLQMVYGIFNGTGAGLPN
                     LDPITFYHDAGLQGTVNFKNVAAGKETLSVKGSVTLTSQVTGLNAVHATGDVTINNPS
                     VVLKEVTANGTVSVSGDGKVLDKLVGLAGVNITQGFVGSVFSNGNISYRTGAPPMGAS
                     NVVSAVAARGSVTINNQNNLTFNDIGSITTTSLSGCGFGVCFKRVEAGSTLTAYPPTL
                     DKAYSKTGITCSPPPALPPPLSLQPATEAVAPSFSGCATTAANTKFRTSTPSLKLIMQ
                     LPPVDMTPLKIDVWEQQAVANYYIRVSGLRTLVTVKNVKRAAISPSPAVALNGEFEFV
                     RSGTLGYLCPLDALGAQLPGCTVAANPKNVFCTDNCWTVQAGGTVAGAAPNTFILEGR
                     VAPGVIFFDGNLNLKFNPLSAAAFLASGYIKTEGNTGKSLALNFAGPSGGTAAGTILP
                     GVCSNLANVQLNLTPSNFCLAGGTYDSTAANKLGNVALMAGGYRRSVTTTTAVYKASD
                     KASETTTANLQADGRTSYVITKVTPNASAGTTTTEVITQQPYTGGDIQLAADNVVFGS
                     LLAGNMLKTEGTSTIYGYVVSSALARTPSTSGGATLNDGGLGLTQNLLSAATNIDHSI
                     QPQYYEGKAVPGAATGTPPSNTGNDVRVLRSRYL"
     gene            complement(5544..6245)
                     /locus_tag="PKZ52_06100"
     CDS             complement(5544..6245)
                     /locus_tag="PKZ52_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59379.1"
                     /translation="MGRRRQTGATLISLLVGMVVSMLAILASLSMFHDLVTTSAEAKT
                     DARQEGDLSLAVLRLDTELQAAGFNMGRSPGEGRNLDFVTAASADGSRHDVAWRFNDG
                     AQMICRRATSSTSNGIYSLDLFSAPAASCTAAWPLAGNVSVDASWTFAERLVYLPLQN
                     LTSQAAAFTQPLITFTSLAANDRACMPYGAIATGVTDVHPILTLGVFDVSTVYSGAAN
                     AVPSRPHSLCLANIF"
     gene            complement(6248..6646)
                     /locus_tag="PKZ52_06105"
     CDS             complement(6248..6646)
                     /locus_tag="PKZ52_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59380.1"
                     /translation="MYTLKRQNGDFLIEAMVGTMITALVSLGMLAISSRVMLTQGEMS
                     RQEAIVTTLREQLHNHNPNVAASNQCALTDDKRKFTLPDKTALTYSVNCPARSVQYSI
                     GGQTYTLSLPMADMTVTNGSTNATYTLGAG"
     gene            complement(6649..7224)
                     /locus_tag="PKZ52_06110"
     CDS             complement(6649..7224)
                     /locus_tag="PKZ52_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNG59381.1"
                     /translation="MAVVTIKMRPGGFTLIELLVVLAVVAFLAFATVPLASGWIRNAQ
                     LERDFAGVQRALGTAKALAMRNAQGVGAAGFTPTAVLCRLNTSLVIYQLDQPNKNDPA
                     SCSRVAASDKPVHSLGQDVSITFGANNQTFCGAQFDARGSVRLCQGANCATCDLSFDP
                     RLDSGAIKVAVAGADATTDASKDQTQLIRAF"
     gene            complement(7228..7698)
                     /locus_tag="PKZ52_06115"
     CDS             complement(7228..7698)
                     /locus_tag="PKZ52_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59382.1"
                     /translation="MRLPRCTLRRQAGFSLIELMVALAIVGILAVVAVPSYSKFIRKG
                     EADTAWGDLQALSLALEGRFRRTLSYPATPATNDTAAVKALVASWGPASKDVAFGFQY
                     QLVDNAAGKYRVSSLGQSTGNFRNCTLILEVIRGTATRYCNDPAACQITCDRAP"
BASE COUNT         1862 a         2234 c         2497 g         1870 t
ORIGIN      
        1 aatgggtttg ctgtacatgg ggttaactca ctggtgaacg ttataaggtg atattgacca
       61 aacacaatgt gtgggtacgg gccaaatccg gacgtggggg gccagcagca ttggcggcat
      121 tgggagccgg ggcataaatg gccgccgtat caaacacatg aattctaaga ttggggtgca
      181 tcggcgctgc cgcgcccggc gccagtggtg cggctgcgcc gaaaggcata caggcgcggt
      241 cattgaaagc atcggaggaa aactcaatta aagggcgcgt aagcgttacc ggcgtgccag
      301 catccaaagc ctgcaagcgg atggacacca actgctcact gcgcgtccat ttggtgtcgt
      361 cgtttaaatc gggcagcgca gcaggccccg gcaaacacaa accggcattt gcctcgaaaa
      421 gttccaaagt gtactgcaat tgcgggatat taaaggtgga caccgcacgc ctacataccc
      481 agttgccgcc atcattgagg cgccaaacaa ctctggaggt gggcgtattg ccgacaaggt
      541 tgttcgccag cgcaaaatcc tgattacgcc ctgcccccgg tgcacggtct acattaaaac
      601 ccgccgccaa taattcctga tctaaacgca gtacgccgag gctgatatcc ccttccatac
      661 gggcatcggc tttggcttca gtggaggtgc gcaccaaatt gtggaacatg gacaggctcg
      721 ccagaatcgc cagcatagag accaccatgc ccaccagtag gctgatcatg gtggcgccgc
      781 gctgcatgtt catgctcatt gcggatcaac tcccaattcg tattccgctg gatctgttgc
      841 tgtggggacc acgatattca agttcgctga ctcaatccgg gcgttataag gcgtgttagt
      901 caccggatga acataaacct ctgtcagtgg cgtgcatcca tcagtggcat tcgcagctat
      961 attcacgccg cgctgcgtga agtccaatcg cctcgctgct gtgaccttat tgttattatt
     1021 cgggttatta cagcgcaccg tggcagctga aaaagggtca cgggtataaa gctcttcccg
     1081 caaagttgcc accacaaatt cctgccgcac catctcttct tgggtgagca tgactcgaga
     1141 ggttattgcc agggttccca ggcttaccag tgccgtaatc agggtgccga tcatggcctc
     1201 gattaagaaa tccccgcttt gctgcttaag gctgtacata tcaaaacgcc cgtatggtgt
     1261 tggactgatc attaggatct ggtctatttg cgttgtttgg gtcttgcgtg gtgtcggcac
     1321 cggctaccac cacacttagg cccgtggcag ggtcaaaaga cgtagtacag gtagtgcacg
     1381 gattgccttg acacaatttc gcccggccac gcgcgttgaa ataagcaccg caaaacgcta
     1441 tttgattgcc aaaggtgatg gccacatcct gccccatgcg ataaaagggt ctggctccag
     1501 cgctaatgtt gttccaattt gcatcggcgc agcttcggtt tgcggcgggc aaatctgcgg
     1561 cgctggccaa gaataaattg tcacccttcc tgcatagaac cgccgcaatg ttatcgcttg
     1621 gtaacggcgt attaggcaga aagttatctg gagcttgaac ccccttagcg ttgcgcattg
     1681 ccagcgcctt agcagttccg agcgcacgct gcaaaccggc aaaatctttt tctaattgag
     1741 cgttgcgaat ccaaccagag gcgagcggta cggtcacaaa ggccaagaag gcaaccaccg
     1801 ctaaaaccac taaaagctca accagcgaga aaccaagagt gcgcttcata ctcggtaagc
     1861 tctttagtta gcgggctgtg cgggcagcgc gtcgcactta actttgcaca cagctggatt
     1921 gttgcagaag cgagtcgcgg ctccacgcac aacctctaat accaaggtgc aattattaaa
     1981 gttactggtg gtttgcccta cggcacttat tcgataggtg gcggtacctt gattgacaag
     2041 tgtgtattga aaattgaagg cggcagccgc tttggtggat ggcccccaac tagacactaa
     2101 atctttaacc gctgctgtgt tgttagtggc aggtgttgga ggataactaa gcacccggcg
     2161 aaaccgccct tcaatagcta ggctcaaagc ctgcaaatcg ccataggcgg catccgcttc
     2221 gccgcggcga ataaagttgg aatagctcgg cactgcgacg gcggcgagga tgccgacaat
     2281 agctatggcc accagcagct cgattagact gaaaccccgt tgcgtgcacg taattttgag
     2341 catgacagcc ccttatgtta ggcattcacg caccagcctt agcccgtaaa tttagatagt
     2401 gaagttttac cagaacccag cagggccgag catcgtgatc ggcctgcccc atgcacgaag
     2461 caactggtgc ggggaacggc gctggttttt agctcctgag ctaagttcgt tgacccgtgt
     2521 tgattttaat tatggttata agaaatcaat aacttagaag gtagcataag atgttcatca
     2581 tagtacctaa gacactaagc tggttcaata aacccgctat ttagtcactt agtttgatta
     2641 tcacctgtcg gtttttcgtg ccgattacgg gaaaaagtgt gcgctggggc ggcagaaaga
     2701 taagaaccat gcctcatttt gcaggcaaaa cgaggcagat tttggctcta aagagactca
     2761 ttatgcggtc gatctggact aagcgccgct tatcgggaaa ggctcaaggt taatgattta
     2821 gatggagccg cataaaactc tggggggttt taaacctaaa aaccgcagtt gaactcgtag
     2881 cgagggtggc taaggcgccg accatatgcg cttttttatc aaaatttggc gaaagcggta
     2941 gtcatctcta gggtgagtca gattttgata aaaaagcgtg cagattcaag cattaggcag
     3001 ccgtagtaga gttcaactgc ggtttttagg ttaaatcatg cggcaaattc agggcatagc
     3061 tactgtaagc gccttaggct gagcgagttg ttacccctgg ccagtcatcc accccgccgt
     3121 ccatggctcc ccatgcaacc atcagccgag cacactcggc tgatggttgc acttataagt
     3181 atcgcgagcg caatacgcgc acatcattac ccgtgttgga aggtggtgtg cctgtggcgg
     3241 cgccaggaac ggctttgcct tcgtagtact ggggttgaat gctgtggtcg atgttggtgg
     3301 cagcggagag gagattttgc gtcagcccca aaccgccgtc gttgagggta gcgccgccgg
     3361 atgtggaggg cgtgcgcgcc agggcggagg agactacata gccgtagatg gtgctagtgc
     3421 cttcggtttt gagcatattg cccgccagta gcgaaccaaa taccacgtta tccgccgcca
     3481 gctgaatgtc gcctcccgtg tagggttgtt gggtgatgac ctcggttgtg gtggtgcctg
     3541 cgctggcgtt aggggtaact ttggtaatca cgtaagaggt gcggccatcg gcttgcaggt
     3601 tagcggtggt ggtttccgag gctttgtcac tggctttgta aacagccgtg gtagtggtta
     3661 ccgagcggcg gtagccaccg gccatcaagg ccacgttgcc cagcttatta gcagcggtgc
     3721 tgtcgtaagt gccaccggct aagcagaagt tactgggcgt gaggttcagc tggacgttgg
     3781 caagattcga acaaacaccc ggcaggattg tgcccgcagc cgtgccccca gaggggcccg
     3841 cgaagttcag cgctaggctt ttacccgtat tgccctcggt tttgatgtag ccagatgcca
     3901 gaaacgcagc agcagagagc ggattaaatt tcaggtttag gttgccgtcg aagaaaatta
     3961 cccccggcgc cactcggccc tccaaaataa aggtgttggg ggctgccccc gccacggtgc
     4021 cacccgcttg gacggtccaa cagttatcgg tacaaaagac gtttttcggg ttggccgcaa
     4081 cagtgcagcc gggcaactgc gcgccaagcg catccaaggg acacaaataa ccgagggtgc
     4141 cagaacgcac aaactcaaat tcaccattta aggctactgc tggactgggt gaaatggcgg
     4201 cgcgtttgac atttttcacc gtaaccaagg tgcgcaagcc gctgacgcgg atgtaataat
     4261 tggcaaccgc ttgctgctcc cagacatcaa ttttaagagg tgtcatgtcg acgggtggca
     4321 gctgcatgat gagttttagg ctcggtgtgc tggtgcgaaa tttggtgttc gctgcggtgg
     4381 tggcacaacc cgaaaaactc ggcgcaacag cctccgttgc tggctgtaga gaaagcggcg
     4441 gtggcaatgc cggtggcggt gaacaggtaa tgcctgtttt gctataggcc ttgtccagcg
     4501 tgggcggata agcggtcaac gtgctgcccg cttcaacgcg cttaaaacac acaccgaagc
     4561 cacagccgga cagcgaggtg gtggttatac tgcctatgtc gttaaaggta aggttatttt
     4621 gattgttgat ggttaccgac ccgcgcgcgg cgacggcgga gactacattg cttgccccca
     4681 tgggaggggc gccggttcgg tagctaatat tgccatttga gaacacgctg cccacaaacc
     4741 cctgggtgat attgacgccc gctaacccca ccagtttatc cagtaccttg ccatcgccag
     4801 agacacttac cgtaccgttg gccgttacct cttttaacac cacactgggg ttgttgatgg
     4861 tgacatcgcc ggtggcgtgt acggcattaa ggccggtaac ctggctggtc aaggtcactg
     4921 agccttttac cgatagcgtc tctttgcccg ccgccacgtt cttaaagttg acggtgccct
     4981 gtagacctgc gtcgtggtag aaggtgattg ggtccaggtt gggcaagcca gcgccggtgc
     5041 cattgaaaat gccgtacacc atttgcagtg tggtgcccac cccagccgtt gagttgactg
     5101 cggccaggtt cacggttacc tcggtgcggg cgggcaagcc gccgctggcg gcaacaagca
     5161 ctcggttggt gaccgtgggg gtaatagtgc ccgccaatgc gggtacaccc gctgcagggg
     5221 ttacattggc actactaatc gccgtcatag gttcattgag aggcaacaca ttcaccaggc
     5281 cgatgggcat ggtggtgagc aactgccgaa acgcctcact gcccgcccag gccaaattct
     5341 gtgcgctcac ctgggcttgg gcggtgatct gtttgtcctg ggcgttttgc acgttaaaca
     5401 tcagggcgag gcccatagcg gtgatgctaa agcccaccag caccgacatg atgatggtgg
     5461 cggcgccgcg ctgtgcgctt aggggttgtg tgtgcatagg tgttctcata atgctcgatg
     5521 agtcgctcgt gagttaggtt gcatcagaaa atattcgcca aacacaggct gtggggcctg
     5581 ctgggtaccg cattggcggc gccggaataa acggtggaga cgtcaaatac ccccagggtg
     5641 agaatcgggt gcacatcggt aacccccgtg gcaatggcgc cgtaaggcat acaggcgcga
     5701 tcgttcgcgg cgagggacgt aaacgtgatt aacggctggg taaaggccgc cgcttggctg
     5761 gttagatttt gcagcggcag gtacaccagg cgctcggcaa atgtccaact ggcatcaacg
     5821 ctgacgttcc cggccaaggg ccaagcggcg gtgcagctgg cggcgggggc actgaagagg
     5881 tcgaggctgt agataccgtt gctggtgctg gaggtggccc ggcggcagat catctgagcg
     5941 ccgtcgttaa agcgccaagc cacgtcgtgg cgggagccgt cggccgaggc ggccgtgaca
     6001 aagtcgaggt tgcggccctc gccgggggag cgccccatgt tgaaccccgc cgcctgtaat
     6061 tccgtatcca accgcagtac cgccaggctc aagtccccct cctggcgggc atcggttttc
     6121 gcctcggcgc tggtggtcac caagtcgtgg aacatggaca gactggccaa tatggccaac
     6181 atggatacca ccatgccgac cagcaagctg atcagggtgg cgccggtctg tcgcctcctg
     6241 cccatgctta gcccgcccca agagtgtagg tagcattggt gctgccgttg gtgacggtca
     6301 tgtccgccat gggcaggctc agagtatagg tttggccgcc gatgctgtac tgcacactgc
     6361 gggccgggca attgacgctg taggtgagcg ccgttttatc cggcagagtg aatttgcgct
     6421 tgtcatcggt cagcgcgcac tggttagacg cggcgacgtt ggggttatgg ttgtgcagct
     6481 gctcccgcag agtagtgaca atcgcctcct ggcggctcat ttccccctgg gtgagcatca
     6541 cgcgggagct gatggccagc atgcccaggc tcaccaaagc cgtaatcata gtgcccacca
     6601 tggcttcaat taaaaagtcg ccgttttgcc gcttgagggt gtacatagtt agaacgctcg
     6661 tatgagttgg gtttggtctt tgctggcgtc ggtggtggca tccgcccccg ccaccgccac
     6721 tttgatcgcc ccgctgtcca agcgagggtc gaagctgagg tcacaggtgg cgcagttggc
     6781 cccctggcac aggcgcacgc tgccccgtgc gtcaaactgc gcaccgcaaa atgtttggtt
     6841 gttagcgccg aaggtgatgc tcacatcctg accgaggctg tggacgggct tgtcgctggc
     6901 ggcaacgcgg ctgcaactgg ccgggtcgtt tttgttgggt tgatccaact ggtaaatcac
     6961 caggctggta ttcaaacggc acagtacagc ggtgggggta aagcccgctg cgccgacgcc
     7021 ctgcgcattg cgcatggcca gggccttagc ggtacccagg gcgcgctgca caccggcgaa
     7081 gtcgcgctcc aactgggcgt tgcgaatcca accggaggcc agcggcacag tcgcaaaggc
     7141 taaaaaggcc accacagcta acaccaccag cagttcaatc agggtgaagc cgcccgggcg
     7201 cattttgatt gtcactacgg ccatggctca gggcgctcga tcacaggtaa tttggcaggc
     7261 ggccgggtcg ttgcaataac gggtggccgt gcctcggatc acctccagaa tcagggtgca
     7321 gttcctaaag tttcccgtgc tctggcccag gctgctcacc cggtatttac cggccgcgtt
     7381 atccaccaat tggtattgaa agccaaaagc cacgtctttg ctcgcaggcc cccagctggc
     7441 aactaaggct ttaaccgccg cggtgtcatt ggtggcgggg gtggccgggt aactgagggt
     7501 gcggcgaaag cgcccctcaa gggcgaggct tagagcctgc aggtcgcccc aggcggtatc
     7561 cgcctcgcct ttgcgaataa atttggagta actgggcacc gccactacgg ccaggatgcc
     7621 gactatggct agggccacca tcaattcaat caggctaaat cccgcctggc ggcgcagggt
     7681 acatctgggt aggcgcatgt gcgtgggtac atctttcaac agttaaacaa tgaccaaggc
     7741 gcaggcaaga ggacaacgcc cacaccacct tttgcggcta aaaccgtttt tggctcaaaa
     7801 accaatactt tagaatttag cagctgacga aatttatggt acttaggtat cattttcgcc
     7861 gcaatcatgg gccaagccga gggtattagg agggcttcac tcgtcctttt tcagcatccg
     7921 ctggtaggtt tatggggtgt tttttagatt aaaaaatcac caatacgcca gggttagaac
     7981 caaacccagc agcgggcggg ggaaaaatgt gaagtggttg ttttttgtcc gctttcagaa
     8041 ggaattaggc gccgttcgtc ggctaaaggc cagctgaggg cctgggcgct gcgtataaat
     8101 tggcctgaga attgcgtttg cggcgcaaca aagtgaggta taggaaagtg cagtaccgga
     8161 aagtgcgata taagaaaggc atacagggcg gcactaaggg atgaatctgt acagccctgc
     8221 ttggggtttc gcctaggcta aggatggtaa tttaattaag ttaagagttg gcggcctatt
     8281 tcaccacggg cagagcctta aattcccctc ctctggaggg gtgccccgaa ggggtggggt
     8341 ggtagatcgc tgcgctgttt tttattcgca gcacgaggta cttgtgggta aaagatctcc
     8401 ttgccctccc tgaaatacaa cagggttctc taccgcaacc accccggcag gcaagcctgc
     8461 cac
//
LOCUS       DAOQGS010000117         8451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3986_length_8451_cov_5.184374, whole genome shotgun sequence.
ACCESSION   DAOQGS010000117 DAOQGS010000000
VERSION     DAOQGS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_8451_cov_5.184374"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PKZ52_06120"
     CDS             complement(<1..353)
                     /locus_tag="PKZ52_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59383.1"
                     /translation="MRTIFSCSAHLHTQGFKLTVLALSLALLTNCGGTDQDRGSAAVN
                     NSAGSETTLKGLAIDGHLARAKVYIDSDNNGVRDAWEPFAFTDNEGYFSYNPNSQKNY
                     CASNATAEESQYCLKP"
     gene            complement(462..1769)
                     /gene="traF"
                     /locus_tag="PKZ52_06125"
     CDS             complement(462..1769)
                     /gene="traF"
                     /locus_tag="PKZ52_06125"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNG59384.1"
                     /translation="MRHLAPLTASLIALPALAQGLASGPTLTTGQTSDHHSVYAGALN
                     PALGELMVNEGEQFRLGFTPSISTITEVGQVDNFVDDLDDLIDLIDDPSLNKDPVQTV
                     LDRFNNILVKMGDEGYVKNSAELHLPLLPVYWKMPLWDGTLMADISLNTQVLARVLDA
                     PLSYDEQNKSFSTATAAYLKGATQTAFALGYARGWFDTAEQKTYGGQLYAGAKLSVYA
                     IELSKQVFLLQKLDGKDIGDVIRDEYDNNSQSTTAAGLDLGVIWASERYRLGATLYNL
                     NQPSFDYGPIGENCQTRAENSVARANCEAAADFAQVRGEIKALESHKKSAYLAVDGTY
                     YWQSNAWVSAALDLASYDDFVGTQNQWLNISGTYSPSTHWLPTVRSGLQKNLAGSKLT
                     QLALGLEFKGVSLDVSWALDSTAIDGSDLPRSFGFSLAIAEQF"
     gene            2012..2500
                     /locus_tag="PKZ52_06130"
     CDS             2012..2500
                     /locus_tag="PKZ52_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HNG59385.1"
                     /translation="MVRLRGVTGCIAGFTVYKRLLGALCGLWLLGTALSVQALPQGEV
                     ALGQYQLLYSAINSTLLPAEMANAYNLKRKADEVLINITVLDTQTKRAVPVGLKGVAK
                     NLLQQSKPLTFREIKETDAVYYLATLRTTEREVFHFLLDVNLPDGRLEKVKFTQELFV
                     NP"
     gene            2523..3110
                     /gene="rdgB"
                     /locus_tag="PKZ52_06135"
     CDS             2523..3110
                     /gene="rdgB"
                     /locus_tag="PKZ52_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNG59386.1"
                     /translation="MRNVVLASNNAGKVAEFQQLLAAVGFTVLPQSQFNLPSVEETGL
                     SFVENAILKARYAAAATGLAALADDSGIEVDALNGAPGIYSARFAGINATDADNNAHL
                     LAQLAGVPEHLRTARYQCVLVYMRHSSDPMPLICSASWEGRILTAPQGEGGFGYDPLF
                     WLPELNCTSAQLPQAQKNAISHRGKAMAALLAALS"
     gene            3120..4244
                     /gene="hemW"
                     /locus_tag="PKZ52_06140"
     CDS             3120..4244
                     /gene="hemW"
                     /locus_tag="PKZ52_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNG59387.1"
                     /translation="MLPPLSLYVHIPWCVKKCPYCDFNSHAAQGALPEADYLAALLQD
                     LPGEIKRAQGRRLESIFFGGGTPSLLSAQFFNALLTQLQKQLEFSPDIEITLEANPGT
                     AELCNFTLLAQVGVNRISLGVQSFNDRHLQQLGRIHSAAEVPQAVAKIRAAGIDNINL
                     DLMHGLPGQTLDEARADLTAAIALAPQHISWYQLTIEPNTIFYNRPPVLPEDDLLADI
                     QAMGEDVLAAAGYAQYEVSAFAKPKKEAAHNINYWQFGDYLALGAGAHGKITWPDRLE
                     RYAKTRLPKDYLAATQFDASVNIVADEQKPFEFMMNGLRLRAGIPRQFWIERCGLPDK
                     VLTPMVDKLIKQGLLEADDDFYRTTALGYRFLNTVLQEFE"
     gene            4399..4596
                     /locus_tag="PKZ52_06145"
     CDS             4399..4596
                     /locus_tag="PKZ52_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG59388.1"
                     /translation="MIESQKNSVMTLDELASYLKVPKSTLYKLVQDGKVPGQKVGKQW
                     RFAKASIDRWLAAEAPTKGAI"
     gene            4680..7475
                     /locus_tag="PKZ52_06150"
     CDS             4680..7475
                     /locus_tag="PKZ52_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNG59389.1"
                     /translation="MASARVDMNPHQVEAAWFALKSPLEKGVLLADEVGLGKTIEASL
                     VMAQKWAEHKRNILLVVPASLRKQWEQELQDKFELPSTIIDSKVAQALKKDGCTNPFF
                     QSEKIIICSYEYLAQQKDKALLISWDLVVFDEAHKLRNVYQKQGSKRAKAIVEATRGA
                     ASRVLLSATPMQNNLIELYGLSQVIDPHFFGDEKSFRALYVNRQKNKDVLQDLKKRIT
                     PLCHRTLRRQVQEEGGIMFTNRYAITQDFTPTAEEWELYTQLSEYLRLPYHQTIDPKA
                     KHLVSIGLRKILASSSFAVAETLKNMLARLAGDQAIDDEAINDLDAKDDWLDINLGAA
                     DTDEELDQDEAEPADLIDAPKRPSNAKYKQEFDLLHHCEYLASKIDDNAKGQALIDVL
                     EKAMAATESLGGQRKAVVFTESCRTQQYLYTLLSKNGYADRIVLLNGTNADTQSQSIY
                     NEWKKRHQGTARISGSKPADMKAALVEKFKSTGADILISTEAGGEGINLQFCSLLINY
                     DLPWNPQKVEQRIGRVHRYGQKNDVVIVNFVNRKNPADERVFNLLHEKLKLFEGVFGA
                     SDEVLGAISSDIDIEKRIYEIYQKCESADDIQTAFDKLQEDLSPTLSVREASARETLL
                     ANFDRDVVANLKNIRDESQIFLQDYEQGLLDLAKAELPEARFEDTHFYYKDKRYDLRW
                     NLAELNGSEFFRLHAVEHLLAWELVKKAQGRYLPPAILHFNYSQLRGQYAALKPYIGK
                     SGVIAALRLRFSFNKGNSHATQVLVVANTDQGETLSQDHATHLLCIPATGTPLPDPID
                     ASVLQQQIDSLIAGQTLIKEQELDTYLEQETEKLERWAQDRRDGLQQRVKELDDEIRQ
                     FKKEARLLATTKDKIDAKRALRILEQKRDDAFSEYHLAKKDIEAEEDRLLDEVSEKLH
                     LTCEALTIFTARWVLNN"
     gene            7497..>8451
                     /locus_tag="PKZ52_06155"
     CDS             7497..>8451
                     /locus_tag="PKZ52_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNG59390.1"
                     /translation="MELEKLAKQLMFHLRLGEDSSIEFKAIVFEGNTLKEPNKDKMAH
                     EIAAFANGQGGCIVLGVEDKTRHLIGIEDTKVALAEEWIVNISQQNINPPVKLHTRLV
                     QLPNQQGDLKTVVYVEIPRSIAVHQSAGRYYQRIGSTKQEMKPEVLARLFQQRSQSRL
                     IRFDETLVPGTSLEDIDKSLKQRFLKPAVVENSLFKKLHLTAQEDDELGLSVCGVLLL
                     TAEPTQFLSNAYIQCVFYSGTARDANDQLDAQDYTGPLDQQINGALDFVKRNMRTAAI
                     KTTGRVDIPQYDVGAVFEAIVNAVAHRDYSMTGRRIRLHMFA"
BASE COUNT         2224 a         2005 c         2103 g         2119 t
ORIGIN      
        1 ggttttaaac aatattggct ttcttccgcc gttgcattac tcgcgcaata atttttttgg
       61 ctattgggat tgtaactaaa ataaccttcg ttatcggtaa acgcaaaagg ctcccaggca
      121 tcgcgcacgc cgttattgtc gctgtcgatg tacactttgg cgcgggctaa atgcccgtct
      181 atcgcaaggc ctttaagcgt agtttcgctc ccggcagaat tattcaccgc tgcgctgccg
      241 cggtcttgat ccgtgccgcc gcagttggtg agtagggcaa ggcttaatgc taaaacggtt
      301 aatttaaaac cctgtgtatg taggtgagcg gagcatgaaa atattgtgcg catatatacc
      361 acttatacgt tgatgaaaac acttgggcac ctgcgcaaat ccacttttcg cgccttagcg
      421 ttacgaaaaa agaatttccg cagatgccca cttactagcg attaaaattg ttcggcaatg
      481 gccaaggaaa aaccaaagct gcgcggcagg tcgctgccat caatggccgt gctatcgagt
      541 gcccaagaca catctaggct tacgccctta aattccaagc ccagcgctaa ttgggtgagt
      601 ttgctaccgg cgagattttt ctgtaatccg ctgcgcacgg taggcagcca atgggtgctg
      661 ggtgaatagg tgccggaaat atttagccac tgattttgcg tgccgacaaa atcatcatag
      721 gaggcgagat ctaaggcggc ggaaacccag gcattgctct gccagtaata ggtgccatcc
      781 acggcaagat aagcggattt tttatggctt tcgagggctt taatctcgcc gcgcacttgg
      841 gcaaagtcgg cggctgcttc acaattggcg cgcgccacgg aattttccgc acgggtttgg
      901 caattttcgc caatggggcc gtagtcaaac gagggttgat ttaaattgta taaagtggcg
      961 ccgagcctat agcgttcact ggcccaaata acacctagat caagcccggc agcagtggtg
     1021 gactggctgt tattgtcgta ttcatcgcga ataacatcgc caatatcttt gccatcgagt
     1081 ttctgcaaca aaaatacctg tttacttaat tcgatggcat aaaccgataa ttttgccccg
     1141 gcgtataatt ggccgccata ggttttttgc tccgctgtat caaaccagcc gcgtgcgtag
     1201 cctaatgcaa aagctgtttg ggtggcgcct tttaaataag cggccgttgc ggtactaaag
     1261 gatttatttt gttcatcata actgagtggc gcatcgagca cgcgggccag cacttgcgtg
     1321 ttaaggctaa tatccgccat taaggtgccg tcccacagag gcattttcca atacaccggc
     1381 agcaggggga ggtgcagttc ggcgctgttt ttcacatagc cttcatcgcc cattttaacg
     1441 agaatattat taaaacggtc cagaacggtt tgtactgggt ctttatttaa actggggtcg
     1501 tcgattaaat cgattaaatc atccaggtca tcaacaaaat tatccacctg gcccacttcg
     1561 gtaatggtgc tgatactcgg cgtaaaacct aagcgaaatt gttcgccctc atttaccatt
     1621 aattccccta aagcggggtt gagtgcgcct gcgtatacac tgtgatgatc ggacgtttgg
     1681 ccggtggtca gtgtggggcc agaggcgagc ccctgtgcta aggctggcag ggcaattagg
     1741 cttgcggtta agggtgctaa atggcgcatg cttactcctt attaggttcc tattatggct
     1801 tgataatatc cactctagcc caagcgccta aaacgccgca tttattcgtg cagatagact
     1861 aaataagtct ttgcgtgatt tttttatgcg gctatttgca gaatggtttt tgacagtttt
     1921 tagtgtgctt aagcctgtgc acgcagggtg cgttagtatt ttggcgcatg ccggggttgc
     1981 tgttatgatg agctttttgc tcgggggatt tatggtgcgc ttacgcggtg tgacgggttg
     2041 tatagctggc tttacagttt ataaacgcct gctgggcgct ctgtgcggct tgtggctgct
     2101 aggcacagcc ctgtctgtgc aggcgctccc tcaaggggag gttgccttag gccaatatca
     2161 attgctttat tccgccatca atagcacctt gttgcccgct gaaatggcca acgcctataa
     2221 ccttaaacgc aaggccgatg aagtgttaat taacatcacg gtattggata cccaaaccaa
     2281 acgagcggtg ccggtaggcc ttaagggtgt ggcgaaaaat ctgctccagc aatccaagcc
     2341 tttaaccttc cgggagataa aagaaaccga cgccgtttat tatttggcta ccctgcgcac
     2401 caccgagcgg gaagtgtttc attttttatt ggatgtaaac ctgccggatg gccgcttgga
     2461 aaaagtaaaa tttacccaag agttatttgt taatccgtaa cactattttt ataggtgtgc
     2521 ctgtgcgcaa tgtagtattg gccagcaata atgctggcaa ggtagctgaa tttcagcagt
     2581 tattggcggc agtgggtttt actgttctgc cgcaaagtca gtttaattta ccctccgtgg
     2641 aggaaaccgg cttaagtttt gtggagaatg ctattttaaa agcgcgctac gccgccgctg
     2701 ccacaggcct tgctgccctg gcggatgatt cgggtattga agtggatgcc ttaaatggtg
     2761 cgccgggaat ttattcggcg cgttttgccg gcattaacgc cacggatgcc gataacaacg
     2821 cccatttatt agcacaacta gccggtgtgc ccgagcattt gcgcaccgcc cgctaccaat
     2881 gtgtgctggt gtatatgcgc cacagcagcg accctatgcc gttaatttgc agcgccagtt
     2941 gggaggggcg gattttaacg gcgccccaag gggaaggcgg ttttggctat gatccattat
     3001 tttggttgcc ggaattaaac tgcaccagcg cgcaattacc ccaagcgcaa aaaaatgcta
     3061 ttagtcatcg cggtaaagct atggccgcat tattagctgc tctatcctag ggttattgcg
     3121 tgttaccgcc tttaagtttg tatgtgcata ttccttggtg tgttaaaaaa tgcccctatt
     3181 gcgattttaa ttcccacgcc gcccagggcg ccctgccgga ggcagactat ttagcggctt
     3241 tattgcagga tttgccgggc gagattaaac gcgcccaggg gcgcaggttg gagagtatat
     3301 tttttggcgg cggcacgccg agtttgctct cggcgcagtt ttttaatgcg ttattaaccc
     3361 agttgcaaaa gcagctggaa ttttcccccg atatcgaaat taccctggaa gccaaccccg
     3421 gcaccgcaga attatgcaat tttactttgc ttgctcaagt cggtgtgaat cgaatttcct
     3481 tgggcgtgca gagttttaac gacaggcatt tacagcaatt aggccgcatt cattcggcgg
     3541 cagaagtgcc ccaagcggta gctaaaatcc gcgcggcggg catcgataat attaatttgg
     3601 atttaatgca cggcctgccg gggcagaccc ttgacgaggc gcgcgccgat ttaaccgccg
     3661 ccattgcgct cgccccgcag catatttcct ggtaccaatt aaccatcgaa cccaacacta
     3721 ttttttacaa ccgcccgccg gtattaccag aggatgattt attggcggat attcaggcta
     3781 tgggggaaga cgtattggca gcggcaggtt acgcgcaata cgaagtttcc gcctttgcca
     3841 agcctaaaaa agaagcggcc cacaatatta attattggca gtttggcgat tatttagctt
     3901 taggcgctgg cgcccacggc aaaatcacct ggccggaccg tttagagcgc tacgccaaaa
     3961 cgcgcttgcc taaggattat ttagccgcta cgcagtttga tgccagcgtc aatattgttg
     4021 cagacgaaca aaaacccttc gaatttatga tgaacggcct gcgcctgcgt gcaggcatcc
     4081 cccgccagtt ttggattgag cgctgcggct tgccagataa ggtgttaacg ccgatggtgg
     4141 ataaattaat taaacagggg ttgctggaag cggatgatga tttctatcgc accactgctt
     4201 tggggtatcg ttttttaaac acggttttgc aggaatttga gtaatcgtcc gcaatacatt
     4261 gcacgcgcgc ctgagccgcc caggctcgcg atacccaggg ggtgagcaag cacacatctg
     4321 atgattgcta tgtcgagcat ttatactttt atgcaatgga ggtaaactgc caaaaaccgc
     4381 caaaatccag taataaccat gattgaatcc cagaaaaaca gcgtgatgac cctggacgag
     4441 ctggcaagct acctaaaggt gcccaagtcc accctttata agcttgttca agacggcaag
     4501 gtgccagggc agaaggtcgg caaacagtgg cggtttgcca aggccagcat agatcgttgg
     4561 ctcgctgctg aagcccccac caaaggagcc atctaaccgt gttcacacct catcaaagcg
     4621 cctattttgc ttactggcta acccaaacag acaagattaa aaaccaagtt tcgcgtgtca
     4681 tggcgtccgc ccgtgtggat atgaaccccc accaagtgga ggcggcttgg tttgcgctta
     4741 aatcccccct tgagaaggga gtgttattgg ctgatgaagt cggcttgggt aaaaccatag
     4801 aagccagttt ggtgatggcg caaaaatggg ccgaacacaa acgcaatatt ctgctggtgg
     4861 tgccagcatc cctacgtaag caatgggagc aggaattaca ggataaattt gaattgcctt
     4921 ccaccatcat tgacagcaaa gtggcccaag ccctaaaaaa agacggctgt accaatcctt
     4981 tcttccaaag cgaaaaaatt attatttgct cctatgaata cctagcacag caaaaggata
     5041 aggcactgtt aattagctgg gatttggtgg tcttcgatga agctcataaa ctgcgcaacg
     5101 tctatcaaaa acaaggcagc aaacgcgcca aagccatagt ggaggcgaca aggggagcgg
     5161 caagccgcgt attgttatcc gcaaccccca tgcaaaataa tttaattgag ttatatggcc
     5221 taagccaagt gattgatccg catttttttg gtgatgaaaa atcctttcga gccctgtatg
     5281 taaatcgcca gaaaaacaaa gacgttttgc aggatctaaa aaaacgcatt acccctttat
     5341 gccatcgcac cttgcgccgc caggtgcaag aggagggggg catcatgttt accaatcgtt
     5401 atgcaataac tcaggatttt acccccacgg ctgaggagtg ggagctatac acccaactat
     5461 cagagtattt gcgcctgcca taccatcaga caattgaccc gaaggccaaa cacctggtca
     5521 gcattggatt acgcaaaatt ttggcgtcct catcgtttgc tgtagccgaa acattaaaaa
     5581 acatgctggc tcgattggcc ggggatcaag ccattgatga cgaggctata aatgatcttg
     5641 atgccaaaga cgattggtta gatattaatt tgggcgcagc ggataccgac gaagaattgg
     5701 atcaggatga ggcagagcct gccgatttaa ttgatgcgcc taaacgcccc tcgaacgcta
     5761 aatacaaaca agagtttgac ttgcttcatc actgtgagta tttggcaagt aaaatagatg
     5821 acaacgcaaa gggccaggcg ctgattgacg tacttgaaaa agccatggcg gcaaccgagt
     5881 ccctcggcgg gcagcgcaaa gcggtagttt ttactgaatc ttgccgtacc cagcaatatc
     5941 tgtacacgct tctaagcaaa aatggttacg ccgatcgtat cgtgctatta aatggcacca
     6001 atgccgatac ccagtcgcaa agtatctata acgaatggaa aaagcggcat cagggcactg
     6061 cacgaataag cggttcaaaa cctgctgata tgaaggcggc cttggtggaa aaatttaaaa
     6121 gcacaggggc ggatatttta atttccactg aggcgggcgg tgagggtatt aaccttcagt
     6181 tttgttcact attaataaac tatgaccttc cctggaaccc acaaaaggta gaacagcgca
     6241 tcggccgcgt acaccgttac ggccagaaaa atgacgtagt tatagttaat tttgtcaacc
     6301 gcaaaaaccc tgctgatgag cgagtgttta atctgctgca tgagaaactt aaattatttg
     6361 aaggtgtatt tggcgcctct gatgaagttt tgggtgctat ttccagcgat attgatatag
     6421 aaaagcgtat ctatgagatt taccaaaaat gtgaatccgc agacgatatc caaacagctt
     6481 ttgacaagct acaagaagat ctaagcccga ctctttcggt gcgcgaggct agcgcacggg
     6541 agactctgct agctaatttt gatcgcgatg tggtggccaa tcttaaaaat atccgcgatg
     6601 agagccaaat atttttacag gattatgagc aaggtttatt ggacttggca aaagcagaat
     6661 tgcccgaagc gcgctttgag gatacccatt tttattataa agataagcgt tatgacctgc
     6721 ggtggaacct ggcggaatta aacggctccg agttttttcg cctgcatgcc gttgaacacc
     6781 ttcttgcatg ggagctggta aaaaaagccc agggcaggta tttgcccccg gcaattttac
     6841 attttaatta ttcgcagttg cgcggccaat acgcggcttt aaaaccctat attggcaagt
     6901 ctggggttat tgcagcgcta aggctcaggt tttcatttaa taaaggcaat agccatgcta
     6961 cacaagtatt ggtggttgcc aatactgatc aaggcgaaac gctgagccaa gatcatgcta
     7021 ctcacttact gtgtattccc gccaccggta cgccgctgcc tgaccccata gatgcctctg
     7081 tattacagca acagatcgat tcgcttattg cggggcaaac cctcataaag gaacaagagc
     7141 ttgataccta cctagaacag gaaacagaaa aacttgagcg ctgggcgcaa gatcgccgcg
     7201 atggactgca gcagcgggtg aaagaattag acgatgaaat acgccaattt aaaaaagaag
     7261 cccggctttt ggccaccaca aaagacaaaa tcgatgctaa gcgagccttg cgtattttgg
     7321 agcaaaagcg cgatgacgcc tttagcgaat atcacctagc caaaaaagat atagaagcag
     7381 aagaggaccg cctgctggac gaagtgagtg aaaaactgca tttaacctgc gaggcgctca
     7441 ctatttttac cgcccgctgg gttttgaata attaatacca aagatcgagg cacgccttgg
     7501 aactggaaaa actggcaaaa caactgatgt tccacctgcg cctaggtgag gattccagta
     7561 ttgaatttaa agccattgtg tttgagggca ataccctcaa agagcccaac aaagacaaaa
     7621 tggcccacga aatagccgct tttgccaatg gccagggcgg ctgtattgtg ttgggcgtgg
     7681 aagataaaac ccgccattta attggcattg aggacacaaa agttgccttg gccgaagaat
     7741 ggattgttaa catcagccag cagaatatta acccgccggt aaaactgcac acccgcctgg
     7801 tgcagctacc caatcagcaa ggggatttaa aaactgttgt ctacgtggaa atcccccgca
     7861 gtattgctgt gcaccaaagc gcgggccgtt attatcagcg catcggctcc accaaacagg
     7921 aaatgaagcc agaggtatta gcccgcctgt tccagcagcg ctcacaatcc cgtttaattc
     7981 gctttgatga aacactagtg ccgggcacct ctcttgagga tatagataaa agcctcaaac
     8041 aacgcttttt aaaacccgca gttgttgaaa atagtttatt caaaaaactg cacctaacgg
     8101 cccaagagga tgatgaactg ggcctttcgg tgtgcggcgt tttactttta accgccgagc
     8161 ctacccagtt tttatccaat gcctatattc aatgcgtgtt ttacagcggc accgcgcggg
     8221 acgccaatga ccaactggat gcccaggatt acaccgggcc attagaccag caaattaacg
     8281 gcgccctgga ttttgttaaa cgcaatatgc gcaccgctgc tatcaaaaca actggtcgcg
     8341 tggatatacc ccaatatgat gtgggcgccg tttttgaagc catagtcaac gcagtggccc
     8401 accgggatta ttccatgacc ggcaggcgca ttcgccttca catgtttgcc g
//
LOCUS       DAOQGS010000118         8445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_3991_length_8445_cov_4.397139, whole genome shotgun sequence.
ACCESSION   DAOQGS010000118 DAOQGS010000000
VERSION     DAOQGS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3991_length_8445_cov_4.397139"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKZ52_06160"
     CDS             <1..449
                     /locus_tag="PKZ52_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59391.1"
                     /translation="PPADISTLDGYFLAAITAQNRNANAEAIALYKSAISWYETQLLH
                     LAEVKNLSLSLNNPQALNNLKNAWQNLPVRSGRAAKLFERQLEFWRWQPTAPAAFAAG
                     LASLQSQLERELTEALSQSLEENNQALNAYLSNCRYGLANTYDKKS"
     gene            433..3159
                     /locus_tag="PKZ52_06165"
     CDS             433..3159
                     /locus_tag="PKZ52_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG59392.1"
                     /translation="MIKNPKFLPWPLCLGLSLVCGCAGTSHKPTIADVDFSRTPQKTE
                     QQTASEQTRSQIVQSYYQYIEKAPKNDKLREDALGRLAALEIDNANLDSENPQLSPEA
                     LNRANNLLQQSLRDYPNSTNKDRVLYQLAQVYEQLGRPAESVQSLKTIIQDYPKSPFI
                     AEAHFRIAEDAFKRGEYLTAESGYSEALLAPNNGLFAERALYKRGWARFKQNIFNDAA
                     DDFLAALRQQTYSDLKELTESKKALINELLRAASLAAARLPEQSLPTLLGDDFVWSYE
                     FFEQLAEVKRKVSTTQALAAMQVFIQRYPQSPRLPLAYLYQLQLYAQLNQWASFDDTL
                     TTLYNQYQPDAAFWQQNPNVDKNTVLTGLRKNMLTAAGQGLSGASAQQLSHANIWSER
                     YLKHFAAFAQQDKMYKQYALLLSANKDNEKALGYYEKSAFDGDIILDKDAAYAAIVTN
                     DQLIQKSSGPTRQTLAAKMARHSKIYAQMYPQDKHSMELLLRAADVNIQEKNSQQVVD
                     LLASLPPGSPLADQQKAAAFKALALADLKRFNDAETAILAAYSSKLSSAENTALADSL
                     ANIVLKQAEEAKSANNTTEAARHYARIATLLPQHDLAAKGLYQAASLSAEQGQWAQAA
                     GFGQEFAQRFPRHELMADVNRLLSSSYLKANNQGKAAESLEALAKNEKDTSVKAAALW
                     QAATLYEQQNDKLAAVRALSQYVDNYPKPFGQYMEALYKLAQLNSGDAKARQVSLDKI
                     QLAANQAAFSDKTERTAYINAWAQLQKANEQYTQFQRVRLVEPLAQNLALKKQALQNC
                     VTLYTQAGANNQLDILAEASFQVASIYEEFAKALLASERPRNLKGDDLEQYNILLEDQ
                     AFPFEDKAIEFHQINLARLKTADNPWLHKSLAALKRLTPARYGRTAKVSFYE"
     gene            3152..3742
                     /locus_tag="PKZ52_06170"
     CDS             3152..3742
                     /locus_tag="PKZ52_06170"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG59393.1"
                     /translation="MNKLIRSSHIALWLLITLLLGACAGQSQKEPSAQTKPAAALRAE
                     DQGAWQASQQLIKQQKWPEAKNTLQNLAKTNPQFPGVLINLAAVECQLQQWQAAGEWL
                     KRLANAQQSPQALNLLGVVAEHSGQINQAEQYYLSAVNSPSAPAEAQYNLALLYDIYW
                     QDPLKALPYYEAYAKARPEDKAASDWINEIKRKAKR"
     gene            3745..4038
                     /locus_tag="PKZ52_06175"
     CDS             3745..4038
                     /locus_tag="PKZ52_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59394.1"
                     /translation="MTYLSAQKIASRVLLWASLALGATGELTWAAEAPANLPEPNPAG
                     ASTGDESPMMLYLVPWNANPESGKNGKKFTLYQPWGSHFDPLTPVQIQQLNAQ"
     gene            4342..5307
                     /locus_tag="PKZ52_06180"
     CDS             4342..5307
                     /locus_tag="PKZ52_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59395.1"
                     /translation="MFLRPLILSLSVFLGVLTSVAVCAEEYQILSASYGTDAHNMEVT
                     DQLKNLASQNRTVKITNSLFGYDPDPGRTKMLRIFARDPNGQVRTFEYKEGKYIDGAQ
                     FTGWSNGNWGQQGWRGGWDGEQRGQERNRGGEYVILQASYGTFERNVDVTGRLRELAR
                     QNRTFRMGNNTFGIDPDPGRIKTLRVYTRGPNGDNRTFEFRENSEVDGNQFADWGGGN
                     WGRGGWKGGWGEDERGRGRHKDRYAGGLAVISARYGEGNRTIDVTQQVRREIRDGRLD
                     FTANNYLAGRDPAPGRTKYLTITYRGEDGRTQVSTTQEGRQVRLP"
     gene            5399..6157
                     /locus_tag="PKZ52_06185"
     CDS             5399..6157
                     /locus_tag="PKZ52_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59396.1"
                     /translation="MTRISHPAIWLGSLLSALLVLAHLTLLPSAHAAPAASKDYPVYS
                     LQRDLRRCAAPACGGWWVTPVNASSQGVLTESLLATAAPLTKPAPEYVANLDFGCAGW
                     TPEQISRFNSLAADRAVLVAGRLLDPSPASTNAALYQYGALQVRDGFAAASSSAANGL
                     YYSVASTGIVCAVAPCPSFQGHLINSSLSQELHNLDFGEGFSPEQIQKAQSLLASGSL
                     LVNGTQQAISGPAGKGVSLSIQQIFWPYPAVQPD"
     gene            6231..6950
                     /locus_tag="PKZ52_06190"
     CDS             6231..6950
                     /locus_tag="PKZ52_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNG59397.1"
                     /translation="MSLRNWDIGLFYWLNNLGRHMPLAAELITHLADHGWTAPLLVIL
                     LAKQPRWLAAALVAALFIHLGVREFKHYFEVLRPCFDPQLAAYVFSAGPDLNIDSYSF
                     PSGHSATASLISVALVALWGRRAQWPALIFALLVAASRSMLGAHFPSDTSAGLALGMI
                     ISVAAFYAGARLQLSPQGERLLALLVWLLAAGLLVFILLAGQHNYPLWFKAASKLGCL
                     LGLGVVGWQVKTHIGPRNSQT"
     gene            7023..>8445
                     /locus_tag="PKZ52_06195"
     CDS             7023..>8445
                     /locus_tag="PKZ52_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59398.1"
                     /translation="MAHTDSLKPLALALLALHLTACNDGGSYKSGSGSSSSSSSGAYS
                     SSSSSSSSSSSSSSSSSSSSSSSSSGALQAQATAFGSKLSALGEQLTLASDAWALGPD
                     AKPTTLSKTGFSVYTFDSDPSADSACTSSSCISNWPPLLVGKLDEVKAPFTQVLRKDG
                     FKQWALRDKPLYLFKGDSSAGDTLGDGKNGLWHLAHSAPISLKTAADSSTYWAAFGSV
                     WRLLATAPQDATGMSLYTFAKDNGSSACTDTCSKNWPPLLAGASDVASAPYSLISRTD
                     SELKQWAYNGKPLYFYTGDSLPGDTKGAGLNSGAWQLARPEAASISPSALGSRLAATG
                     PVYIASLNSSGEEQLTAAAQLQGRSLYTFDKDSAGASSCTKTNDCISKWPPLLAAPGS
                     LASAPYSLIERAAGLKQWALDGKPLYFFAQDTGAASTQGDGVGGVWHLARSAPVLIKN
                     LEGSGSYFAASNAALTAAGVADPA"
BASE COUNT         2067 a         2527 c         2140 g         1711 t
ORIGIN      
        1 ccccccccgc cgatatttcc accctagacg gttattttct cgcggccata acggcgcaaa
       61 accgcaatgc caacgccgag gccatagccc tgtataaaag cgccatcagt tggtatgaaa
      121 cccaattgtt gcacctggcc gaagttaaaa atctctcctt aagccttaac aacccgcaag
      181 cgttgaataa tctaaaaaac gcctggcaaa acttacctgt gcgctcaggc cgcgcggcaa
      241 aattattcga gcgccaacta gaattttggc gctggcagcc tactgcgcca gcggcctttg
      301 cagccgggct tgcgagcctg caaagccaat tagagcggga attaaccgag gccttaagcc
      361 aaagtttgga agagaataat caagccttaa acgcttattt aagcaattgt cgctacggac
      421 ttgccaatac ctatgataaa aaatcctaaa tttttgccct ggcccctatg cctgggttta
      481 agcctagtgt gcggctgtgc gggcaccagc cataaaccca ccatcgccga tgtggatttt
      541 agccgcacgc cgcaaaaaac cgagcaacaa accgcctcgg agcaaacccg tagccaaatt
      601 gtgcagtctt attatcaata catcgaaaag gcgcccaaaa acgataaact gcgcgaggat
      661 gccctaggcc gcttagcagc cttagaaatt gacaacgcca atttagacag cgaaaacccc
      721 caattaagcc cggaggcctt aaaccgcgcc aacaatttat tgcagcaaag cctgcgcgat
      781 taccctaact ccaccaataa agaccgcgtg ttatatcagc tggcccaggt ctatgaacaa
      841 ctgggccgcc cggctgagag cgtgcagagt ttaaaaacca taattcagga ttaccctaaa
      901 tcgccgttta ttgccgaggc gcattttcgc atcgccgaag acgcctttaa gcgcggtgaa
      961 tatttaacgg ctgaaagcgg ctatagcgaa gccctgctag cacccaataa tggtttattt
     1021 gccgagcgcg ctttatacaa acgcggctgg gcgcgcttta aacaaaatat ttttaacgat
     1081 gcggcggatg attttttagc ggccctgcgc cagcagacct acagcgattt aaaagaactc
     1141 accgaaagta aaaaagccct gattaacgaa ttgttgcgcg ccgcaagcct tgccgcagcg
     1201 cgtttgcccg aacagagcct gcccacttta ttgggtgatg attttgtctg gagttatgaa
     1261 ttttttgaac aattggccga ggtaaaacgc aaggtttcca ctacccaagc cctcgccgcc
     1321 atgcaggtat ttattcagcg ctacccacaa tcgccgcgcc tgcccctggc ctatttgtat
     1381 caattgcagt tgtacgcgca gttaaaccaa tgggcgagtt ttgacgatac cctcaccacg
     1441 ctttacaacc agtatcagcc tgatgctgcc ttttggcagc aaaaccccaa tgtggataaa
     1501 aacaccgtgc tcaccggcct gcgcaaaaac atgctcaccg ccgcaggcca gggtttaagc
     1561 ggcgccagcg cccagcaatt aagccacgcg aatatttgga gcgagcggta tttaaaacat
     1621 tttgccgcct ttgcccaaca ggacaaaatg tacaaacaat acgcgctgtt gttatcagcc
     1681 aataaggata atgaaaaagc cctcggctat tatgaaaaat ccgcctttga tggcgatatt
     1741 attttagata aagacgccgc ctacgccgcc atagtcacca acgatcaact gattcaaaaa
     1801 tccagcggcc ccacccgtca aaccctcgct gcgaaaatgg cccgccacag taaaatttat
     1861 gcgcagatgt atccgcagga taaacacagc atggaattat tactgcgcgc agccgatgta
     1921 aacattcaag aaaaaaacag ccagcaggtg gtggatttat tggcgagttt accgccgggc
     1981 agcccccttg ccgaccagca aaaagccgct gcctttaaag ccttggcact ggcggattta
     2041 aaacgcttta acgatgcgga aacagctatt ttagcggcct attccagcaa actgagcagc
     2101 gcggaaaata ccgcgctggc cgatagtttg gcgaatattg tgttaaaaca agccgaggaa
     2161 gccaaaagcg ctaataacac taccgaagcc gcccgccact acgcccgcat cgcaaccctt
     2221 ttaccgcaac acgatttagc tgctaaaggc ctgtaccaag cggccagcct cagcgccgaa
     2281 cagggccaat gggcccaggc ggcgggcttc gggcaggaat ttgcccagcg ctttccccgc
     2341 catgaattaa tggccgatgt aaaccgtttg ttatccagca gttatttaaa agccaataac
     2401 cagggtaaag cggcggaaag tttagaggcc ctggcgaaaa acgaaaaaga taccagcgta
     2461 aaagccgccg cgctttggca agcggccact ttatacgaac aacaaaacga taaactcgcc
     2521 gccgtgcggg cattaagcca atatgtggat aattacccca aaccctttgg gcaatatatg
     2581 gaagcgcttt ataaactcgc ccaattaaac agcggcgatg ccaaggcccg ccaagtgagt
     2641 ttggataaaa ttcaattggc cgccaaccaa gcggcgtttt ccgataaaac cgagcgcacc
     2701 gcctacatta atgcctgggc gcaattgcaa aaagccaatg aacaatacac acaatttcag
     2761 cgcgtgcgtt tagtggagcc cttggcgcaa aacctggcgt taaaaaaaca ggccctgcaa
     2821 aattgtgtca ccttatacac tcaagctggc gccaataatc aattggatat tttggcggag
     2881 gcgagctttc aggtggcgag tatttatgag gagtttgcca aagccctgct agcctctgag
     2941 cggccgcgca atttaaaagg tgatgattta gagcaataca atattttatt agaggatcag
     3001 gccttcccgt ttgaagacaa ggccattgaa tttcaccaaa ttaatttggc ccgcttaaaa
     3061 accgccgata acccctggct acacaagagc ctcgcagctt taaaacgctt aacgcccgcc
     3121 cgctatggcc gcacagctaa ggtgagtttt tatgaataaa ttaatccgca gcagccatat
     3181 agccctttgg cttttaatta ccttattgct gggtgcctgt gcggggcaat cgcaaaaaga
     3241 acccagcgca caaactaaac ctgccgccgc cctgcgggca gaagaccaag gggcctggca
     3301 ggcaagccaa caattaatta agcaacaaaa atggcccgaa gcaaaaaata ccctgcaaaa
     3361 cctggccaaa accaatccgc aatttcccgg tgtattgatt aacctcgccg cagttgaatg
     3421 ccaattacaa cagtggcagg cggctgggga gtggttaaag cgcctagcca atgcccagca
     3481 atccccccag gcattaaatt tattgggcgt agtggcagag cattccggcc aaattaatca
     3541 ggccgagcaa tattatttaa gtgccgttaa tagcccttcg gcgcctgccg aagcccagta
     3601 caatttagcg ctgctatacg atatttactg gcaggatccg ctgaaagcgc tgccctatta
     3661 cgaagcctac gccaaagccc gcccggaaga taaagcggcg agtgattgga ttaatgaaat
     3721 taaacgcaaa gcgaagcgtt aaccatgact tatttatccg cccaaaaaat tgccagccgt
     3781 gtgctgttat gggccagcct cgcccttggc gcaaccggtg agttgacctg ggccgctgag
     3841 gcccccgcca acctgccaga gcccaacccg gcaggcgcca gcaccggcga tgaatcccct
     3901 atgatgctgt atttagtgcc ctggaacgcc aacccggaga gcggtaaaaa tggcaaaaaa
     3961 tttaccctct accaaccctg gggcagccat tttgacccgc tcacgccggt gcaaattcag
     4021 caattaaatg cgcaataata aaatgccccg cattacacat tttgtgtgat gctttaattt
     4081 tcatgtcgga agtcgctgcg ctcttccaac ctacgccaga aaaggatttt ccggcaactt
     4141 aatgacattg ccactacagg ctcctcctca agttaatgac attggcttag cagcgcctag
     4201 aattgagatg tttaaaagct acacagcgat acaggataaa cgccgtcgat aggaatgcca
     4261 cgcacagcgc ggttaatggc acaattaaat gcacacattt ttttagccca tggcccctcg
     4321 cctaatttcg gagtctttac catgttcttg cggcccctca ttttaagcct cagtgttttc
     4381 cttggcgttc tcaccagtgt tgctgtgtgc gccgaggagt atcaaattct cagcgccagt
     4441 tacggcaccg atgcacacaa tatggaagtg accgatcaat taaaaaatct cgccagccaa
     4501 aatcgcacgg tcaaaattac taactcccta tttggctatg acccagaccc aggccgcaca
     4561 aaaatgctgc ggatttttgc ccgcgacccc aacggccagg tgcgcacctt tgaatacaaa
     4621 gagggtaaat acattgacgg cgcacaattt accggctgga gcaacggcaa ctggggccag
     4681 cagggttggc gcggcggctg ggatggcgag cagcgcggcc aggagcgcaa tcgcggcggc
     4741 gagtatgtga ttttgcaagc cagctacggc accttcgagc gcaatgtgga tgtaaccggg
     4801 cggctgcggg aattggcccg gcaaaatcgc accttccgca tgggcaataa cacctttggc
     4861 atagacccag acccaggccg catcaaaacg ctgcgggttt acacccgcgg ccccaatggc
     4921 gacaaccgca cctttgaatt tagagaaaac agcgaagtgg acggcaatca gtttgccgac
     4981 tggggcggcg gcaattgggg gcgcggaggc tggaaaggcg gctggggcga ggatgagcgc
     5041 ggtcgcggca ggcataagga tcgctatgcg ggcggcttgg ccgttatctc cgcccgctac
     5101 ggcgaaggca atcgcaccat tgatgtcacc cagcaggtgc ggcgggagat tcgcgatggg
     5161 cgcttggact ttaccgccaa caattacctt gcgggccgcg acccagcgcc gggccgcacc
     5221 aaatacctca ccattaccta tagaggcgag gatggccgca cccaggtgag caccactcaa
     5281 gaggggcggc aggtgcgcct gccctaagca acaggggcgc aatttgtgac ctacccggcc
     5341 aacaacctgc gggccttgcg ctaacctggg cgctggcatt catacacgag agggctttat
     5401 gacgcgtatt tcacaccctg caatctggct gggcagcctg ctaagtgcgc tgctggtatt
     5461 ggcacatctc acgctactgc ccagtgccca tgcggcgccc gccgccagca aggactaccc
     5521 ggtgtacagc ctgcagcggg acctgcgccg ctgcgccgcc cccgcctgcg gcggctggtg
     5581 ggtaaccccg gtgaatgcca gcagccaagg ggtgcttacc gaatccctat tagcaaccgc
     5641 cgcacccctc accaaaccgg cccctgagta cgtggcgaac ctagacttcg gctgtgcagg
     5701 ctggacgccg gagcagatca gccgctttaa cagcctcgcc gctgaccgtg cagtgctggt
     5761 ggcgggccgc ttattagacc caagccccgc cagcaccaac gccgccctct accaatacgg
     5821 cgccctgcaa gtgcgcgatg ggtttgcggc cgccagcagc agcgccgcca atggcctgta
     5881 ttacagcgtt gcatccaccg gtattgtctg cgccgtggcg ccctgcccgt cttttcaggg
     5941 ccatctcatc aacagcagcc tcagccaaga actgcacaac ctggattttg gcgagggctt
     6001 tagccccgag cagattcaaa aggcacagag cctgcttgcc agtggcagcc tattagtgaa
     6061 cggcacacaa caggccatca gcggcccggc gggtaagggt gtgagcctga gcatccagca
     6121 aattttctgg ccctatccgg cggtgcaacc tgactaagcg ggacgaacgg cttcgcccgc
     6181 ggcacctgct tctgctgctg ggcggcttac tggctgccgc cgcgctgctg ttgagcctac
     6241 gcaactggga tataggcctg ttctactggc tgaataacct gggccgccac atgcccctgg
     6301 cagcagagct gatcacccac ctagccgacc acggctggac ggcgccgctg ctggtaattc
     6361 tgctggccaa acagccgcgc tggttggcag ccgcgctagt tgccgcccta ttcattcacc
     6421 ttggcgtgcg ggaatttaaa cactactttg aggtactgcg gccctgtttc gacccgcaac
     6481 tggccgccta tgtatttagc gccgggccag atttaaacat cgatagctac tcattcccct
     6541 ccggccacag cgccaccgca agcctaatat cggtggcctt ggttgccctc tggggcaggc
     6601 gcgcccagtg gcccgccctc atcttcgccc tactggtggc cgcctcccgc agcatgctgg
     6661 gcgcccactt ccccagcgac accagcgctg gtttagccct cgggatgatc atcagtgttg
     6721 ccgcgttcta cgccggcgct cggctgcaac tatcccctca aggcgagcgg ctcttggccc
     6781 tgttggtgtg gctgctagcc gctgggctgc tggtgtttat cctgctagca ggccaacaca
     6841 actatcctct ctggtttaaa gcggccagca aactgggttg tttgctgggg ttgggggttg
     6901 tgggctggca ggtcaaaact catattgggc cgcgcaattc ccaaacttaa atttccctag
     6961 ctgcaacctt ttcctccgcc tgcctgtaca acctgtcacc taatcattca caggattgtt
     7021 ttatggctca cacagactcc ctcaaacctt tggccctggc cctactcgcc ctgcacctta
     7081 ccgcctgtaa cgacggcggc agttacaaaa gcggcagtgg ctccagtagc tcatcttcta
     7141 gcggcgctta ttcaagcagc tcgtcttcca gcagctcatc aagcagcagt tcatccagta
     7201 gcagctcaag ctcttccagc agttcatcag gtgcgctgca ggctcaagcc acggcctttg
     7261 gcagcaagct tagcgccctt ggagaacagt tgaccctagc ttcagacgcc tgggctttag
     7321 ggccagatgc taagcctacg actttgagca aaaccggctt tagcgtttat accttcgata
     7381 gcgaccccag cgccgattcc gcctgcacca gcagctcctg catcagcaac tggccgccgc
     7441 tgttggtggg caaactggac gaggtaaagg cgcccttcac ccaagtactg cgcaaagacg
     7501 gctttaaaca atgggccctg cgggataaac ccctgtatct atttaaaggc gacagcagtg
     7561 ctggcgacac cctgggggat ggcaaaaacg gcctgtggca tctggcccac agcgccccta
     7621 tcagcttaaa aaccgccgcc gacagcagca cctattgggc ggccttcggc agtgtgtggc
     7681 gcttgcttgc aaccgccccc caagatgcca caggcatgag cctctacact ttcgccaaag
     7741 acaacggcag ctccgcatgc acggatacct gttccaagaa ctggccgccc ctcttggcag
     7801 gcgcaagcga tgtggccagc gccccttaca gcctgatcag ccgcaccgat agcgagttaa
     7861 aacaatgggc ctacaacggc aaacccctgt atttttacac tggcgatagc ctccctggcg
     7921 acaccaaagg cgcaggcctt aatagcggcg cctggcaatt ggcccgcccg gaggccgcct
     7981 ccatcagccc ctccgcccta ggtagccgcc tggccgcaac aggcccggtg tatatcgcaa
     8041 gccttaatag cagcggtgaa gagcagctca ccgctgccgc ccaactccaa ggccgcagcc
     8101 tctatacctt cgataaagac agcgctggcg cctccagctg cactaaaacc aacgactgca
     8161 tcagtaaatg gccgccgctc ctggccgccc ctggcagcct ggcaagcgcc ccctattctt
     8221 taatcgagcg ggcggcgggc ctgaaacaat gggctctgga tggcaaaccc ctttacttct
     8281 ttgcccagga taccggcgcg gccagcaccc agggcgacgg cgtgggcggt gtatggcacc
     8341 tagcccgcag cgccccggtc ttgattaaaa acctggaggg cagcggcagt tattttgccg
     8401 cctccaacgc agctctcacc gcagcaggag ttgccgaccc cgccc
//
LOCUS       DAOQGS010000119         8423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4003_length_8423_cov_6.108748, whole genome shotgun sequence.
ACCESSION   DAOQGS010000119 DAOQGS010000000
VERSION     DAOQGS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4003_length_8423_cov_6.108748"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKZ52_06200"
     CDS             complement(<1..376)
                     /locus_tag="PKZ52_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59399.1"
                     /translation="MKDLLNLLKNQGQPDEFDGIRIGLASPEMIRAWSFGEVKKPETI
                     NYRTFKPEREGLFCAKIFGPVKDYECLCGKYKRMKHRGIICEKCGVEVTLAKVRRERM
                     GHIELASPVAHIWFLKSLPSRIG"
     gene            complement(455..4540)
                     /gene="rpoB"
                     /locus_tag="PKZ52_06205"
     CDS             complement(455..4540)
                     /gene="rpoB"
                     /locus_tag="PKZ52_06205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNG59400.1"
                     /translation="MAYSYTEKKRIRKDFGKLPHIMDVPYLLAIQLDSYRKFTQADLP
                     PQNRFDIGLHAAFKSVFPILSYSGNAALEYVSYNLGKAAFDVNECVLRGATYAVPLRV
                     KVRLIIYDKESSTKAIKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDLVFVRIDRRRKLPASILLRALG
                     YSSQQMLEMFYETNKFRIEADQSLKLELVPARLRGDVASFDITDREGKIIIEEGKRIT
                     PRHIRQMEKAGVDELTVPRDYLLGRFLARDVVDTNTGEVVIECNTEITNEVVNGLIKA
                     GVKEFETIYTNELDCGPFIADTLRSDSARTQLEALVEIYRMMRPGEPPTKESAETLFQ
                     NLFFSPERYDLSAVGRMKFNRRLGREAEQGEGTLSNDDIVDVLKTLIAIRNGKGVVDD
                     IDHLGNRRVRSVGEMAENQFRVGLVRVERAVKERLSMAESEGLMPQDLVNAKPVAAAV
                     KEFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVRDVHATHYGRVCP
                     IETPEGPNIGLINSLATYARTNSYGFLESPVRRVVNGKVTNDIDYLSAITESQYVIAQ
                     ASATLDAEGRLIDELVSVRHQMEFALMPPSEVQYMDVSPRQVVSVAASLIPFLEHDDA
                     NRALMGSNMQRQAVPTLKAQKPLVGTGMERNVASDSGVCVVAKRGGVVDRVDAGRIVI
                     RVANNEVEAGEAGVDIYNLTKYTRSNQNTCINQRPIVRLGDTVSRGDILADGPSVDLG
                     ELALGQNMRIAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELTCIARDTKLGSEEI
                     TADIPNVGEGALSKLDESGIVYIGAEVGPGDILVGKVTPKGETQLTPEEKLLRAIFGE
                     KASDVKDTSLRVPTSTKGTVIDVQVFTRDGLEKDQRSREIEKTQLNKVRKDLNEEYRI
                     VEQATFERLRAALVGQIASGGKTVKKGAPVTDEMLDELDKKDWFKLRMNEEHLNEQLE
                     KAEAQLAERRKELDERFEDKKRKLETGDDLAPGVLKIVKVYLAIKRRIQPGDKMAGRH
                     GNKGVISVIMPVEDMPNDENGVPVDIVLNPLGVPSRMNVGQVLEMHLGLAAKGLGEKI
                     NGMLSQQKAVVEVRGFLEEIYNKTGSARDKEDLASLSDNEVLELARNLVDGVPMATPV
                     FDGAKESEIKALLRLADMPESGQMQLYDGRTGDAFQRHTTVGYMYMLKLNHLVDDKMH
                     ARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVAGRT
                     KMYKNIVDGDHRMEPGMPESFNVLVKEIRSLAINIELENEA"
     gene            complement(4739..5107)
                     /gene="rplL"
                     /locus_tag="PKZ52_06210"
     CDS             complement(4739..5107)
                     /gene="rplL"
                     /locus_tag="PKZ52_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNG59401.1"
                     /translation="MSLTKEDIINAIAEMSVKDVVELITAMEEKFGVSAAAAVAVAAG
                     PAAAAVEEQTEFTVILASAGDKKVNVIKAVRELTGLGLKEAKDLVDGAPKPVKEAIPK
                     ADAEEAKKKLEEAGATVEIK"
     gene            complement(5168..5662)
                     /gene="rplJ"
                     /locus_tag="PKZ52_06215"
     CDS             complement(5168..5662)
                     /gene="rplJ"
                     /locus_tag="PKZ52_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNG59402.1"
                     /translation="MALGLEGKKAIVAEVHEVAKGALSAVVADSRGVTVGKMTALRKQ
                     ARASGVWVKIVRNTLARRAVEGTAYECLVESFVGPTLIAFSNEHPGAGARILADFARE
                     NDKFEIKAAAFEGQIVNVGMLATLPTYDEAISRLMATMLEAAAGKLVRTIAAIRDQKE
                     EQAA"
     gene            complement(5967..6665)
                     /gene="rplA"
                     /locus_tag="PKZ52_06220"
     CDS             complement(5967..6665)
                     /gene="rplA"
                     /locus_tag="PKZ52_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNG59403.1"
                     /translation="MAKLSKRQREIRAKVDSTKLYSIDEAVALLKELSKVKFAETFET
                     AINLGIDPRKSDQVVRGATTLPHGTGKDVRVAVFTQGANAEAAKAAGAEFVGMEDLAE
                     QVKGGMMDFDVVIADPAAMRVVGQLGQVLGPRGLMPNPKTGTVTPDVVTAVKNAKAGQ
                     VRFRADKGGIIHGGIGKISFSAQAVKENLEALITDLKRLKPATSKGIYLRKIALSSTM
                     GPGLNIDLASLDVK"
     gene            complement(6667..7098)
                     /gene="rplK"
                     /locus_tag="PKZ52_06225"
     CDS             complement(6667..7098)
                     /gene="rplK"
                     /locus_tag="PKZ52_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNG59404.1"
                     /translation="MAKKIEAYIKLQVKAGQANPSPPVGPALGQKGVNIMEFCKQFNA
                     QTQGMELGSPVPVIISVYSDRSFTFTMKTPPASFLLKKAAGLKSGSPRPNTQKVGKVT
                     RAQLEEIAKAKEPDLTAADMDAAVRTIAGSARSMGLDVEGL"
     gene            complement(7240..7773)
                     /gene="nusG"
                     /locus_tag="PKZ52_06230"
     CDS             complement(7240..7773)
                     /gene="nusG"
                     /locus_tag="PKZ52_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNG59405.1"
                     /translation="MAKRWYVVHAYSGFEKKVAASIKERIELHGMQEHFGDVLVPTEE
                     VIEMRGGQKRKSERKFFPGYVLVNMELNDDAWHLVKETPRVMGFIGGKADKPAPITEA
                     EAQLILRRVDDSVDKPRPKTIFEPGEMVRVIDGPFNDFNGVVEEVNYDKNRLRVAVLI
                     FGRSTPVELEFSQVEKA"
     gene            complement(7783..8151)
                     /gene="secE"
                     /locus_tag="PKZ52_06235"
     CDS             complement(7783..8151)
                     /gene="secE"
                     /locus_tag="PKZ52_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNG59406.1"
                     /translation="MTTKVEAPEFRLDFVKWLVIVALIALAVFGNSYFSDKPVIVRVI
                     GVILLGAAAVGVALFTEKGHAFLGLLKAARIELRKVVWPTKPEANQTTLLVLGVVAVA
                     SLILALIDSILGWITSQVIQ"
     gene            complement(8201..8276)
                     /locus_tag="PKZ52_06240"
     tRNA            complement(8201..8276)
                     /locus_tag="PKZ52_06240"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8241..8243),aa:Trp,seq:cca)
     gene            complement(8329..>8423)
                     /locus_tag="PKZ52_06245"
     CDS             complement(8329..>8423)
                     /locus_tag="PKZ52_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59407.1"
                     /translation="APIAMEDGLRFAIREGGRTVGAGVVAKILA"
BASE COUNT         1896 a         2307 c         2148 g         2072 t
ORIGIN      
        1 aaccgatacg gctgggcaga gattttaaga accagatgtg agctaccggg cttgccagtt
       61 caatatggcc catgcgctcg cggcgcacct tggccagagt cacttccacg ccgcattttt
      121 cacagatgat gccgcgatgt ttcatgcgct tgtacttgcc gcacaggcat tcgtagtctt
      181 ttaccggccc gaagatcttg gcgcaaaaaa ggccttcccg ctcgggcttg aaggtgcggt
      241 agttgatagt ttcgggtttt ttcacctcgc cgaaggacca ggcgcggatc atttccgggg
      301 acgcaaggcc aatacgaata ccgtcgaact cgtcgggttg gccttggttt ttcagtaaat
      361 tcagcaaatc tttcaagagc gttctcctgt gggcgcggag caaagcgggg agaatccccg
      421 ccgtctttta tcaattgtca cgttgatcgg ttgatcaggc ttcgttttcc agctcgatgt
      481 tgatggccaa ggagcggatt tctttcacca atacgttgaa agactccggc atgcctggct
      541 ccatacggtg gtcgccatcc acgatgtttt tgtacatctt ggtacggccg gccacgtcat
      601 cggacttaac ggtaagcatt tcttgcaggg tgtaggcggc gccgtatgct tccagcgccc
      661 acacttccat ctctccgaaa cgctgaccac caaactgggc tttaccaccc aagggttgct
      721 gtgtaaccaa gctgtaggaa ccggtcgaac gggcgtgcat cttgtcgtct accaagtggt
      781 tcagcttcaa catgtacatg tagccaaccg tggtgtggcg ctggaaggcg tcaccggtac
      841 ggccatcgta cagttgcatc tgaccggact ctggcatatc cgccaagcgc agtagagctt
      901 tgatttcaga ctctttggcg ccgtcaaata cgggcgtagc catggggaca ccgtccacaa
      961 ggttacgcgc cagctccaat acttcattgt ccgaaaggct ggccaggtct tctttatcgc
     1021 gggcgctgcc ggttttgttg tagatctctt ccaagaagcc gcgcacttca accacagctt
     1081 tttgctggct caacatgccg ttgatttttt cacccaggcc cttagctgcc aggcctaagt
     1141 gcatctccag cacctgcccc acgttcatcc gcgacggtac acccagaggg ttgaggacta
     1201 tgtccaccgg cacaccgttt tcatcatttg gcatgtcttc cacgggcatg atgaccgaga
     1261 taaccccttt gttaccgtgg cgtccggcca ttttgtcacc gggttggatg cggcgtttga
     1321 tcgccagata aaccttaacg atttttagta cacctggagc taaatcatcg ccggtttcca
     1381 acttgcgttt tttatcttca aagcgctcat ccagctcttt gcggcgctcg gccaattggg
     1441 cctctgcctt ttccagctgc tcgttcaaat gctcttcgtt catgcgcaat ttgaaccagt
     1501 cttttttatc caactcatcc aacatttcgt cggtgactgg agcgcctttt ttcacggttt
     1561 tgccgccgct ggcgatctgg ccaactaagg ctgcgcgcaa gcgctcaaag gtggcctgtt
     1621 ccacaatgcg gtattcttca ttgaggtctt tgcgcacttt gttcagctga gttttttcaa
     1681 tttcccgtga gcgttgatct ttttccaaac catcgcgggt aaagacttgt acgtcgatga
     1741 ccgtaccctt ggtgctagtg ggtacgcgca gggaggtgtc ttttacgtcg gaggcttttt
     1801 caccgaagat cgcccgcaag agtttctctt ctggggtcaa ttgggtttca cccttaggtg
     1861 ttaccttgcc caccaggata tcgccggggc ccacttccgc acctatatag acgatgccgg
     1921 actcgtcgag tttggacaga gcgccctcgc ccacgttggg aatatccgca gtaatttctt
     1981 cactgccaag cttggtatcg cgggcgatac aggtcagctc ttggatgtga atggtggtga
     2041 agcggtcttc ttgtaccacg cgctcggaca ccaaaatgga gtcttcgaag ttataaccat
     2101 tccagggcat gaacgcgatg cgcatgtttt gccccagggc caactcacct aggtctacag
     2161 atgggccgtc ggccaaaatg tcaccgcggg atacggtatc gcccaaacgc acaattgggc
     2221 gctggttgat acaggtgttt tggttggagc gggtgtattt ggtgaggttg tagatatcca
     2281 caccggcttc accagcttcc acttcgttgt tggcaacgcg aatcacgatg cgaccagcgt
     2341 ccacacggtc cactacccca ccgcgtttag ctaccacaca cacgccggag tcagaggcta
     2401 cattgcgctc cataccggta cccactagcg gcttttgggc cttcaaggta ggtacggctt
     2461 gacgttgcat gttcgaaccc atcaaggcgc ggttggcgtc atcgtgttcg aggaacggaa
     2521 tcaatgaggc ggccaccgat actacttggc gcggcgacac gtccatatac tgcacttccg
     2581 atggcggcat cagggcgaat tccatttggt gacgcaccga taccagctca tcgatcaaac
     2641 ggccttcagc gtccaaagtc gcggatgcct gggcgattac atattgggac tccgtaatag
     2701 ccgacaggta atcaatatcg ttggtaacct tgccattaac cacgcgacgc accgggcttt
     2761 ctaaaaagcc gtagctgttg gtgcgggcat aggtggccag agagttgatc aaaccgatgt
     2821 ttggaccttc aggcgtttcg ataggacaca cacggccgta gtgggtggcg tgtacgtcgc
     2881 gtacttcgaa gccggcgcgc tcacgggtca aaccgccagg ccctaaggcc gatacgcgac
     2941 gcttgtgcgt cacttcggag agcgggttgt tttggtccat aaactgcgac agctggctgg
     3001 agccgaagaa ctctttcacc gcggcggcaa ctggtttggc attgaccaaa tcctgcggca
     3061 tcaagccttc gctctcagcc atggacaaac gctctttcac ggcgcgctca acacgtacca
     3121 agccgacgcg gaattggttt tccgccatct cgcccactga acgcacacgg cggttaccca
     3181 agtgatcgat atcatccacc acgcctttac cgttgcggat ggcgatcagg gtttttaaca
     3241 cgtcgactat gtcgtcgttg gacagagtgc cctcgccctg ctcagcctcg cggcctaaac
     3301 ggcggttgaa cttcatgcgg cccacagcgg atagatcgta gcgctcaggg gagaagaaca
     3361 ggttttggaa cagagtttcc gccgactctt tggtgggcgg ctcgccaggg cgcatcatac
     3421 ggtagatttc taccagggct tccagctggg tgcgcgcgga atcgctgcgc agtgtgtcgg
     3481 cgatgaacgg accacaatct agttcgttgg tgtagatggt ttcgaactct ttcacaccgg
     3541 ctttaatcag cccgttcacc acttcattgg tgatttcggt gttgcactcg atcaccactt
     3601 ctcccgtatt ggtatctact acatcgcggg ctaagaagcg gcctaagagg tagtcgcgag
     3661 gtacagtgag ctcatcaaca ccggcttttt ccatctgacg aatgtggcgt ggagtaatcc
     3721 gcttgccttc ttcgataatg attttgcctt ctctatcagt gatgtcaaag ctggccacat
     3781 caccgcgcaa gcgcgcaggt accagctcca atttaaggct ctgatcggct tcgatgcgga
     3841 atttgttggt ctcgtaaaac atctccaaca tttgttgtga gctgtagccg agggcgcgca
     3901 gtaaaatact tgcaggtaat ttgcgacggc ggtcgatgcg cacgaatacc aggtctttcg
     3961 ggtcgaattc aaaatccaac caggagccac ggtaaggaat aatacgggca gaataaagca
     4021 acttgccgga ggagtgggtt ttgcctttat cgtgctcgaa gaacacaccg ggtgaacggt
     4081 gtaactggga gacgattaca cgctcggtac cgttgataac gaaggtaccg ttgtcagtca
     4141 tgagcggaat ctcgcccatg tagacttctt gttctttaat gtctttgatg gctttggtag
     4201 acgattcttt atcgtaaata atcaaacgca ctttgacgcg caggggcaca gcgtaggtgg
     4261 caccgcgcag cacacactca ttcacgtcga aagcagcttt gccaaggtta tagctgacgt
     4321 actctaaagc ggcgttgccc gaataactta gaatcgggaa aacagattta aatgccgcgt
     4381 gcaaacctat atcaaaacgg ttttgggggg gcaggtctgc ctgggtaaat ttgcggtaag
     4441 aatccaactg aattgccagc agatatggca catccataat gtgtggcaat ttgccgaaat
     4501 ccttacggat acgttttttc tcagtgtatg agtaagccat cagtattcct cagcttgttc
     4561 acggggtgtt gtctaggtaa gacgggggaa gcgcacttcc tggtaaccgt ggtcttgcac
     4621 gcgggcagtt aaaaaactac cacttcaatc attgctgact gcaaacggaa aaaggccagc
     4681 aggcgaaatc gcctgccagc cccgcgccaa accgagattt ggcgtaatac aacacaactt
     4741 acttgatctc gacagttgcg ccggcttctt ccaacttctt cttggcttct tcagcgtcag
     4801 ccttaggaat cgcttcctta actggcttag gagcgccgtc aaccaggtct ttagcttctt
     4861 tcaagccaag gccagtcaat tcacgaacgg ctttaattac gttcactttc ttatcgccag
     4921 cagaagctaa gataacggtg aactcagttt gctcttcaac ggcagcggca gctgggccag
     4981 cagcaacagc tacagcagca gcagcgctaa cgccgaattt ctcttccatt gcagtgatca
     5041 gttctactac atctttaact gacatttcag cgatggcatt gatgatatct tctttagtca
     5101 gagacatgac tcaatcctat ataaacgagc ggggtgctca ataaattgct tggtacaaac
     5161 agcccaatta ggccgcttgc tcttcttttt ggtcgcgaat ggccgcaata gtacggacca
     5221 atttgcctgc agctgcttct aacatcgttg ccatcaagcg agagatcgct tcgtcgtagg
     5281 ttggcaaggt tgccagcata ccgacattaa cgatctgacc ttcgaaggcg gccgctttaa
     5341 tctcaaactt gtcgttctca cgagcgaaat ccgccaggat acgcgcgccg gcacctgggt
     5401 gctcgttgga gaatgcgatt aaagtggggc ctacaaagct ctcaacgaga cattcgtagg
     5461 cggtgccttc tacagcacga cgggccagtg tgttacggac gatttttacc caaacgccgc
     5521 ttgcacgagc ctgtttgcgc aaagctgtca ttttgcccac ggttacaccg cgagaatcag
     5581 caacgacagc ggacaatgcg cctttagcaa cttcatggac ttcagcgaca atcgcttttt
     5641 taccttcaag tcctagtgcc acgggatttc tccagttgtg ataggcgagc ctatcgttgt
     5701 tttcacactc aagcccgagg gcctgagtca ttgatttggt gatgtcattc aaatgtgcag
     5761 cggcaaaaac aaatatttgc agtgccagca caacaatctg aacggggtct caccatctgc
     5821 gtaggcccca aattaattaa ctagggatta aagagccctg ccctgcctac ggtctttgac
     5881 agttcagcga gctgaacccc aaagtcgttt accggctcgg gaatcggtgg ttgcccaccg
     5941 cccaacccgg gtattagctt taaaacttat ttaacatcca aagaggcgag gtcgatgttc
     6001 agaccagggc ccatggtgga gctaagagcg atcttgcgca ggtaaatacc tttggaagtc
     6061 gcaggcttta agcgctttaa atcggtaatc agggcttcta agttttcttt cactgcctga
     6121 gcgctgaagg aaatcttgcc gataccaccg tgaatgatgc cgcctttgtc agcgcgaaag
     6181 cgcacttgac cagctttggc atttttcaca gcagtaacca catcgggtgt tacggtgccg
     6241 gttttggggt ttggcattaa gccgcggggg cccaatactt gacctaattg accgacgacg
     6301 cgcatggcgg caggatctgc aatcaccacg tcaaaatcca tcatgccgcc ctttacttgc
     6361 tcggcgagat cttccatacc gacgaattct gcaccggcag ccttagcggc ttcggcgttg
     6421 gcgccttggg taaacacagc tacacgcacg tctttaccgg taccgtgggg cagagttgtc
     6481 gcgccgcgca ccacttggtc ggatttgcgc gggtcgatgc ccaggttaat ggcggtctcg
     6541 aaggtttcag cgaatttaac tttggataat tctttcagca gggcaaccgc ttcgtcgatg
     6601 ctgtacagct tggtggaatc aaccttggcg cggatttcac gttgacgttt ggacaactta
     6661 gccatcttat aaaccctcca catccaagcc catggaacgc gcggaaccgg cgatggtgcg
     6721 cacagcggcg tccatatcag cagcggttaa atccggttct ttggctttgg cgatttcttc
     6781 taattgtgca cgggtaacct tgcccacttt ttgagtgttg gggcgcgggc taccgctctt
     6841 aagacctgcc gctttcttca ataagaaaga agccggtggt gtcttcatag tgaaggtaaa
     6901 gctgcggtcg ctgtacacac tgatgattac tggtacaggt gaacccaatt ccataccttg
     6961 agtctgggcg ttaaattgct tacagaactc catgatgttc acaccttttt gacccagagc
     7021 agggcccacc ggtggacttg ggttggcctg accggccttc acttgcagct taatataagc
     7081 ttctattttc tttgccattg ttacttcccc ctattgggtt aaacgcctcc cagcagcggc
     7141 gactgctgat cacaggctcc cctgttttgt tgccagcagc taccggcgac aaaacgtgaa
     7201 aagccgccgc aggaacccct gcggcggccg tgaaaatgct taggctttct cgacttggct
     7261 gaactccagc tcaacaggcg tggaacggcc aaatatcaaa acggctacgc gcaagcggtt
     7321 cttatcgtag ttaacttctt ccacgacgcc gttaaagtca ttaaagggtc cgtcgataac
     7381 gcgcaccatt tctcctggct cgaagatagt tttcggccgg ggcttatcca ccgagtcatc
     7441 cactcggcgc aaaattagtt gagcttccgc ttcggtgatc ggcgcaggct tatccgcctt
     7501 accgccgata aagcccatca cacggggcgt ttctttcacc aagtgccagg cgtcgtcgtt
     7561 aagctccata ttgaccaata cataaccggg gaaaaacttc cgctccgatt tgcgcttctg
     7621 gccgccgcgc atttcaatca cttcctcagt gggcaccaat acgtcgccga aatgctcttg
     7681 catgccgtgt aactcaatgc gctccttaat ggaagccgct acttttttct caaagccgga
     7741 ataggcgtga accacgtacc aacgcttagc catagggttc tcctactgaa taacctggga
     7801 agtgatccaa ccgaggatcg agtcgatcaa agcgaggata aggcttgcaa ctgccaccac
     7861 acccaatacc agcagcgtgg tttgattcgc ctcaggctta gtaggccaca ccactttgcg
     7921 cagctcgata cgagcagcct tcaacaagcc caagaaggca tgacccttct cggtaaaaag
     7981 cgccacgccc acagcggcag cacccagcaa aataacgccg atcacgcgca caattaccgg
     8041 cttatcgctg aaataagaat ttccaaacac cgccaaggcg ataagcgcta cgattaccag
     8101 ccacttaacg aaatccaagc gaaattcagg cgcttctact ttggtagtca tagtcgactc
     8161 aatggaagga ttaggatacc gacaggaagt accgcagata tggcaggcca ggagggactc
     8221 gaacccccaa cacccggttt tggagaccgg tgctctacca attgaactac tggcctaaac
     8281 tacaactgac ggcccgaagg ccgccagctt cttactcact taatactatt aagcaaggat
     8341 tttggctacc acgcctgcac ccacagtgcg gccaccttcg cggatcgcaa agcgcagacc
     8401 gtcttccatc gcaatcggtg cga
//
LOCUS       DAOQGS010000120         8378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4030_length_8378_cov_4.052385, whole genome shotgun sequence.
ACCESSION   DAOQGS010000120 DAOQGS010000000
VERSION     DAOQGS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_8378_cov_4.052385"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..302)
                     /locus_tag="PKZ52_06250"
                     /pseudo
     CDS             complement(1..302)
                     /locus_tag="PKZ52_06250"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
     gene            884..1543
                     /locus_tag="PKZ52_06255"
     CDS             884..1543
                     /locus_tag="PKZ52_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNG59408.1"
                     /translation="MPQPLNAVIFDHDGTLVNSEPVHHACWLKAIEQYNAEFSLDEYV
                     TYLTGIPSSSSAQWIIERFKLPTDPATLYARKQAHVDEYLQSHAFPLMPHAESLVRYC
                     FEAGLKLAVASGAGAREIHRSLNAHGLNRWIKVVASKNDVARNKPAPDVYLLAIEQLG
                     ESANGCIAIEDSNVGQAAALAANLTCLRLHPVAPHPQVVGVRSLEDAKAWLHSRLQRQ
                     E"
     gene            1595..2710
                     /locus_tag="PKZ52_06260"
     CDS             1595..2710
                     /locus_tag="PKZ52_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNG59409.1"
                     /translation="MDSELSEIDRQTAEDLFEDFSSHHSHAEQAIAELLILPSDAALH
                     QLFREVHTIKGNAAMFAFDEIVHFIHAIENVISALRTGQLQMTKPIGEVIMMSLDRAR
                     DLHEVYLFKRVVENLNIANIQAALDQLAAARPETAAEIATTNAINQLTNHFAKPCEDT
                     DEAPKTSAYFSNDLHIKQDLIFFRSLASELDKQNIFWDQRSALIFRWSQKINALADYP
                     IDPSQLATAVYLHDVGMSFLPSDIINKASKLSALESQQLHKHPTWGMEFVRRMTQWQE
                     AALMISQHHERFDGAGYPAGIAGTDIHSGARIIAILDAFYAIINSRADRALRRSILRA
                     VSEINACAGSQFCPTWVDYFNQVIREESREGLLGQAV"
     gene            3127..6825
                     /locus_tag="PKZ52_06265"
     CDS             3127..6825
                     /locus_tag="PKZ52_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59410.1"
                     /translation="MNCALSRRPRHPFLVGLFFTSLILSGCGSSSSPGGSSGGSSSSG
                     GGIASSSSSSGASVTSSSSSSSSSTSAGKSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSGSPEACTTPIVPTPNSLRLLAKGSTCANLAYAEYVPAGYSPSGRYPLIIALHGDG
                     QSGAGSTVDVAKLDDDGLPAAIAAGSWDTQKRFVVLAPQMNYLTRTALEVRNFIAFAK
                     ANYAIDPNRIYLTAYSGGGGPFYRYMETYDGADIAAAAPISTVYSFTSDSSEPCSYKQ
                     VPLWIFHGAADTTVGVYSNATAPYDGLKKCVPAPKVAPKISLYSNVAHDAWSRTYNLS
                     GLNTAVETARDTYNQSLYDWFLQFTLATGTGSSSSSSSSTSSSSSSSSSSSSSSGASS
                     SSSSSSSSSSTSSSGTPLSKIDPEFGALSLVDSFDPTNPGSRETQAEPANSSSVQTLL
                     GRPALVLPPQAGMASYAAFRLGKAAGLVPGRAYVLEVDYPDDVPRSLILLNRGADITR
                     TIATGKSLGDYREQYAYPNPESLAYPQSQKWLTARFYFYLHDRFQPLAAVRNEADTRR
                     PFGPEDGFWVALGNPNPKGEPINQGAAMGNVRLYAVSDDSQAALKTQLPPAGIPHRRT
                     FWREEMNDSVAMCVRGDSKVSTDPNSAAYATSCNPATGTSPGTTTNTWLEYKMQLAKV
                     LGFNVFTKDLLEFGHNQGMDMSAYGGDKLFYGARLAFWPEMVKKAGEHNLEVLPYFEY
                     YGSMGAGEFSTTNCPSEDATGNSFCATATGNSRYQCKKPWQQTQAKCFLPSYGSQLNC
                     RPLTRANRYTPYSWAETACVDVTDPEALTDVKKLIGANVLDLKERAQFAGAWFRTRVG
                     SWPINFADAARERYATDRHVAAPSLEQLRQTPSLLADYRAWWQEKRRAYLTAIRDHMR
                     TGSSGGVPGASVLFSSYHEEGLPIPTANYADTRVITDDVPAWTVINADARWKYRYSPA
                     TPADWLSNQRFAKMLFTLNLPTDTQLAGTGSFDEMSHGTPPADPEHYQQDSGLYMSMP
                     FNRQYSVADDNLLKQFTTKEGAALEHHFALNEDDGTGDISADQANSSFNSWPMSGHFG
                     YFVSDMERSSPYTMLAEVRALASTDVNWIGYLSSSSFNTAAPQDLRRFNAAYLAWPAL
                     PSAKVPAASPDPQVIVRDMATPAGTFVAVFNTAMVSKTLNLDLSKTRLGVRSQIQNRV
                     TGKTVATPSGQLTIELPVAGFSVFYVAD"
     gene            complement(6930..8105)
                     /locus_tag="PKZ52_06270"
     CDS             complement(6930..8105)
                     /locus_tag="PKZ52_06270"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNG59411.1"
                     /translation="MTVKLMSQLDLAGKRVLIREDLNVPIQDGVITSDARIRASLPGI
                     KLAVAAGAKVILMSHLGQPAEGEFDANLSLKPVAEHLTKLLGQSVQLISDFSKGVEVA
                     AGQVVLLENVRFNKGEKKDEDALAKAYASLCDIFVMDAFGTAHRAQASTHGVAKFAPI
                     ACAGPLLEAELSALEKSLAKPERPLVAIVGGSKVSTKLTVLDALSKVADILVVGGGIS
                     NTFVAAAGNEVGNSLYEKDLIPEAQRLCKTTQVIFAEDVRTTKDGFKQWSHSSKTEVK
                     KTNEIAVDEEIIDYGPVTAAKVAEIIKNAKTVIWNGPCGVFEFDAFAKGTEVVARAIA
                     DSAAFSIAGGGDTLAAIDKFDLADKISYISTGGGAFLEYVEGKELPAVTILKARANG"
     gene            complement(8170..>8378)
                     /locus_tag="PKZ52_06275"
     CDS             complement(8170..>8378)
                     /locus_tag="PKZ52_06275"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246778.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNG59412.1"
                     /translation="KEAVLPLAVTARVAIEAAHEDYWYKYVGLDGRTVAMGGFGESAP
                     GNVLLEYFGFTLDNVVNTVKELLA"
BASE COUNT         1934 a         2462 c         2097 g         1885 t
ORIGIN      
        1 gggtaattca tttccgagcg gttggtgagg gtaaaatcca cgtcctcctc caagtggccc
       61 aggcgcatcc gtaggcgtac caccgggcgg gcgtcgattt cttccgccga ggcctggcgg
      121 attttggcgt tgcgagccag gggcgcttca aattcttgtt cttttacatt ggtgccctgc
      181 tcgccctcgg cattaacttg cattgtaaaa cgcacccagt ttttaccgtc ccgctcaaag
      241 cgttcaattt tggtggcgtg aatggaagac agggttgcgc cggtatccac gcgggctttg
      301 agctttttat tgagcgcctc aatccatacc cactccaccc ggccgataac taatttggcg
      361 gagtctttgc tggtatcaac ttcgggttta acttctttgc ggggtttttt cgctggcgct
      421 tcggcggcac tgggtaggct cgccacgctg gctgtaacgg gggcactgtt aaaacttagg
      481 gtgctcaatt gcatattggt catgcgcagt tgagccgcca aatcacccac tgcctgggtc
      541 atgtccgtct gctgttgagc cagggcaacg agctgttcgg actggctttt taaggcgcgc
      601 tcctgattat tcagctgctc cgcctggcgc tgctgccctt cactgagggc attcattttg
      661 atgtcattgg ctgcgcagcc ggagatactc agcagcagca aacttaaaag ggtggcaatt
      721 acacgctctg tcacgaacaa tacctgggcc gttgcaggcc aaaaatatag gccaaacacg
      781 gccttttagg gcgcggcagt atagaatgcc cccgccgagc tgtgaaccgc ttagcgccta
      841 tttttaacgc ctcaataccc tacctacctc gcgagattgt tgaatgcctc agccgctaaa
      901 tgccgtaatt tttgatcacg atggcaccct ggtaaattcc gagcctgtgc accacgcctg
      961 ctggctaaaa gcaattgagc agtacaacgc cgagttttcc ttagacgaat atgttactta
     1021 cttaaccggt atcccctctt ctagctccgc ccaatggatt atcgagcgct ttaagttacc
     1081 caccgacccc gccacacttt atgcgcgcaa acaggcccat gtggatgagt atttacagtc
     1141 ccacgccttt cctttaatgc ctcatgcaga aagcttagtg cgctattgtt ttgaagcggg
     1201 tttaaaactt gcggtggcaa gcggcgctgg ggcgcgggaa atccaccgct ctttaaatgc
     1261 tcacgggctt aaccgctgga ttaaggtggt ggccagcaaa aacgatgtag cccgcaacaa
     1321 accggcgccg gatgtgtatt tattagctat tgagcaattg ggggaaagtg ccaacggctg
     1381 tatcgccatt gaagatagta atgtgggaca ggctgcggcc ctggcggcca acctaacctg
     1441 cctgcggctg cacccggtgg caccccatcc gcaggtggtg ggcgttaggt ctttagagga
     1501 cgcaaaggcc tggctgcaca gccgtctgca gcggcaggag tgaataattg cagctaagct
     1561 gtaggggtta ctcataccgg gcgaggcacc caaaatggac tctgaactca gcgaaataga
     1621 ccgccaaacc gccgaggatt tattcgagga ctttagctcc caccacagcc atgccgagca
     1681 ggcgatcgcc gagttattaa tcctgccctc cgacgccgcc ttgcatcagt tatttcgcga
     1741 ggtgcatacc atcaaaggca atgccgctat gtttgccttt gatgaaatcg tgcattttat
     1801 ccacgctatc gagaacgtca tcagcgccct gcgcaccggg caattgcaga tgactaagcc
     1861 catcggcgaa gtgattatga tgagcctgga tcgcgcccgg gacctgcacg aggtgtattt
     1921 atttaagcgc gtggtggaga acctgaatat cgccaatatt caggctgccc tggaccaatt
     1981 ggcagcggca cgccccgaaa ccgccgccga gattgccacc accaatgcca tcaaccagct
     2041 caccaaccac tttgccaagc cctgtgaaga taccgatgag gcgcccaaga cttccgccta
     2101 tttcagcaac gatttacaca tcaaacagga tttaattttt tttcgctcct tggcttccga
     2161 gctggataaa caaaatatat tttgggatca gcgctcggcg ttaatttttc gttggtcgca
     2221 aaaaatcaac gccctggccg actaccccat agatccgtcc caactggcca cggcggtcta
     2281 tttacacgat gtgggtatga gttttttgcc ctccgatatc attaataaag ccagcaaatt
     2341 atccgcctta gaaagccaac aactgcacaa acaccccacc tggggcatgg agtttgtgcg
     2401 ccgcatgacc caatggcagg aggccgcttt aatgattagc caacaccacg agcgctttga
     2461 cggtgccggt tatccggcgg ggattgcggg cacggacatt cacagcgggg cgcgcatcat
     2521 tgcgattttg gatgcctttt acgcgattat taattcccgc gccgatcggg ccttacgccg
     2581 ctccattctg cgcgccgtat cggaaattaa cgcctgtgcc ggcagccagt tttgcccgac
     2641 ctgggtggac tattttaacc aggtgattcg cgaggagagc cgcgaagggt tgcttgggca
     2701 ggcggtttaa gctaaaagcc gcacaactat tcagcacctt ttttaagcac ctatttagtt
     2761 atttttaggt gataggcgag tgctttaatg ctgcgagccc ttattccccc cgcagtgatg
     2821 aagacagcct attgcagacg cgccgtgaat acatccgtgt aggctcttca aaaagatcac
     2881 tctttttatt ggcctgcaat aaccagcctt cacccgctgc tgtaccgcga attgattctg
     2941 cggcttgact attagccgcc cgcttggacg gcggcaacag gggcttttta acgaataaat
     3001 ccgaccagaa gtgcataaat ttacgggtat ttatctcaaa ttcgcatcaa caatcgcccc
     3061 tgggcgagac tttcccgttt agcaggctaa gctaagccta aatttagttg cgaagcgaaa
     3121 cctgccatga attgcgctct ttctcgccgc ccccgccacc ctttcctggt tggtttattt
     3181 tttaccagcc taatacttag cggctgcggc tctagcagct cgccgggtgg gagcagtggt
     3241 ggtagctcgt ccagcggcgg tggtatcgcc agcagtagtt catcatcggg cgcttctgtt
     3301 accagttcat cgagtagttc cagtagcagc accagcgcgg ggaaatcttc tagttcttct
     3361 agttcttcta gttcttcctc cagttcgtcc agctcttctt cgtccagttc atcctcgtcg
     3421 agcagcagct ccagctcatc tggcagcccg gaggcttgca ctactcccat agtgcccacg
     3481 cccaacagct taaggctgct cgccaagggc agcacctgtg ccaaccttgc ttacgccgaa
     3541 tatgtccccg ccggttacag cccaagcggg cgatacccgc taattattgc cctccacggc
     3601 gacggccaga gcggcgcagg ctcgactgta gatgtagcca aactcgatga cgacggcctg
     3661 cccgccgcca tagcggcagg cagctgggat acacaaaaac gctttgtggt gctggcgccg
     3721 caaatgaatt atttaacccg taccgccctt gaggtacgca attttattgc cttcgccaaa
     3781 gccaactacg ccatcgaccc caaccgcatt tacctaactg cctactccgg cggtggcggc
     3841 cccttctacc gttatatgga aacctacgac ggtgccgaca tagccgccgc tgcgcctatc
     3901 tccaccgtat acagcttcac cagcgatagc agcgaaccct gcagctacaa gcaggtgccc
     3961 ctgtggattt tccacggcgc ggcggatacc actgtggggg tttacagcaa tgccacagcc
     4021 ccctacgacg gcctgaaaaa atgcgtgccc gcacccaagg ttgcgcccaa aatcagcctc
     4081 tactccaatg ttgcccacga tgcctggagc cgcacctata acctcagcgg cctcaacacc
     4141 gccgttgaaa cggcccgaga tacctacaac caaagtctct acgattggtt tttgcaattt
     4201 accttggcca ctggcaccgg cagttcgtca agctctagtt cgtccacttc tagctcatcc
     4261 agcagttcaa gctcgtccag cagctcaagc ggggcgagtt caagctcttc aagttcaagc
     4321 agcagttcaa gcacaagttc ctccggcacg cctttaagta aaatcgaccc tgagtttggt
     4381 gcgctaagcc tagtggacag cttcgacccc accaaccccg gcagccgcga aacccaggcg
     4441 gagcctgcca acagctctag tgttcaaacc ctgctcggcc gcccggccct agtactgccg
     4501 ccccaagccg gtatggccag ctatgcggca tttaggttgg gcaaggccgc aggcttagtg
     4561 cccggcagag cctatgtatt agaagtggat tacccggatg atgtaccgcg cagcctaatt
     4621 ctgctcaacc gcggcgccga catcacccgc accatcgcca ccggtaaaag tctcggcgat
     4681 taccgcgagc aatacgccta ccccaacccc gaatccctag cttacccgca aagccaaaag
     4741 tggctgacgg cgcgctttta tttttacctg cacgatcgct ttcaacctct agcggcggtg
     4801 cgcaacgagg cggatacccg cagacccttt gggccggagg atggcttttg ggtcgctctg
     4861 ggcaacccca accccaaagg tgagcccatc aaccagggcg cggccatggg caatgtgcgc
     4921 ctgtatgccg taagtgacga cagccaggca gcgctcaaaa cccagctgcc cccagcggga
     4981 attccccacc gccgcacctt ttggcgggag gagatgaacg actcggtggc catgtgtgtg
     5041 cggggggatt ccaaagtcag taccgacccc aactcagccg cctacgccac cagctgtaac
     5101 cccgccaccg gcacaagccc aggcaccacc accaacacct ggctggagta caaaatgcag
     5161 ctggccaagg tgctcggctt taacgtgttt actaaggatt tattggaatt cggccacaac
     5221 caaggcatgg atatgagcgc ttacggcggc gataaactct tctacggcgc gcgcctggcc
     5281 ttctggccgg agatggttaa aaaagccggg gaacacaatt tagaagtgct gccctatttt
     5341 gagtattacg gctccatggg cgcaggggaa ttctccacca ccaactgccc cagcgaagac
     5401 gccaccggta atagcttctg tgccacagct actggcaaca gccgctacca gtgcaaaaaa
     5461 ccctggcaac aaacccaggc taagtgcttc ttacccagct atggcagtca attaaactgc
     5521 cgcccgctaa cccgggccaa ccgctacacc ccatactcct gggccgaaac cgcctgtgtg
     5581 gatgtgactg acccggaagc ccttaccgat gtgaaaaaac tcatcggcgc caatgttctg
     5641 gatctcaaag agcgggctca atttgccggc gcctggtttc gcacccgtgt gggcagctgg
     5701 ccaattaatt ttgccgacgc cgcccgcgag cgctatgcca ccgaccgcca tgtggccgcc
     5761 ccaagccttg agcaattgcg ccaaaccccg agcctgttag cggactaccg cgcctggtgg
     5821 caggaaaaac gccgcgccta cctaaccgcc attcgcgacc acatgcgcac cggcagcagc
     5881 gggggggtgc ccggcgccag cgtactgttt agcagctacc acgaagaggg cctgccgatc
     5941 cccaccgcca attacgccga cacacgggtc atcaccgatg acgtaccagc ctggacagtg
     6001 atcaacgccg atgcgcgctg gaaataccgc tactcgcccg ccacccccgc cgattggctg
     6061 agcaaccagc gctttgccaa aatgcttttc accctgaacc tacccaccga tacacaactg
     6121 gcgggcacag gcagctttga tgaaatgagc cacggcaccc ccccggcaga ccctgagcac
     6181 taccagcagg acagcgggct atacatgagt atgccgttta accgccaata cagtgtggcc
     6241 gatgacaacc tgctgaagca gttcaccacc aaagaggggg cagctttaga gcatcacttt
     6301 gcccttaatg aagatgacgg caccggggat atcagcgccg accaagccaa cagcagcttt
     6361 aatagctggc ccatgagcgg gcattttggt tatttcgtca gcgatatgga gcgctccagc
     6421 ccctatacca tgctcgccga agtgcgcgct ctagccagta ctgacgtgaa ttggatcggt
     6481 tatttatcca gctccagctt taacaccgca gccccccaag acctgcgccg cttcaacgcc
     6541 gcctacttag cctggcccgc cctacccagc gccaaagttc ccgcggcaag cccagaccca
     6601 caggtgatcg tgcgggatat ggcgacacct gcaggaacct tcgtggctgt atttaatact
     6661 gctatggtca gcaaaacatt gaatttagac cttagtaaaa cgcgtttggg cgtgcgcagc
     6721 cagattcaaa accgggtgac ggggaaaacc gtggcaacgc ccagcgggca gctgactatt
     6781 gaattacccg ttgcgggttt tagtgtgttt tacgtggcag actaatgatt tgctaggcaa
     6841 caaaattaaa gaactcaatt aattgccatc aaatatatta gacataaaaa aagcggccga
     6901 ggccgctttt ttattcgtgc accaaacatc tagccgtttg cacgggcttt taaaatggtt
     6961 actgccggta attctttacc ctcaacatat tctaaaaacg caccgccgcc agtggaaatg
     7021 taggagatct tgtcggccaa gtcgaattta tcaatggctg ccaatgtatc gccaccgcca
     7081 gcgatagaaa acgcagcgga atcggcaatt gcacgcgcaa ccacttcagt gcctttagca
     7141 aatgcatcaa attcaaatac accgcaaggg ccgttccaaa taacggtttt ggcgttttta
     7201 ataatctcgg ctactttggc agcggtcacc gggccgtagt cgataatttc ttcgtcaacg
     7261 gcgatttcgt tggttttttt cacttcggtt tttgagctgt gagaccactg tttaaaacca
     7321 tctttagtgg tgcgcacatc ttcggcaaaa ataacctggg tggttttaca caggcgttgg
     7381 gcctcgggga ttaggtcttt ttcatacaga gaattgccca cttcattgcc agccgccgcc
     7441 acgaaggtat tggaaatacc gccgcccact accagaatat cggcaacttt tgaaagcgca
     7501 tctaatacgg ttaatttagt agaaaccttc gagccgccca caatcgccac caatgggcgc
     7561 tcaggtttag caagggattt ttcaagggcg cttaattccg cttctaataa ggggccagcg
     7621 caggcgatgg gggcaaattt agccacgccg tgggtggagg cttgggcgcg gtgagcggtg
     7681 ccaaaggcgt ccatcacaaa aatatcacac aggctggcat aggccttggc aagagcgtct
     7741 tcgtcttttt tctcgccttt gttaaagcgc acattttcca gcaacactac ttggcctgcg
     7801 gccacttcaa cgcccttact aaaatcgctg attaattgca cgctctggcc gagtaatttc
     7861 gttaaatgct cagcgacggg ttttaggctt aaattagcgt caaattcgcc ttcagccggt
     7921 tggccgaggt gcgacattaa aatcactttg gcgccggccg ccacggccaa tttaattcct
     7981 ggcagcgaag cgcggatgcg agcatcggag gtaataacgc catcttgaat cggtacgttt
     8041 aaatcttcgc gaattaatac acgcttgccc gccaggtcta attgggacat gagttttaca
     8101 gtcataaatt tcacctaaat aaatagttca tacaattaaa aaagccgggt tgccccggcc
     8161 tgtttggcgt tacgccaata attcttttac ggtgttcacc acgttatcca gggtaaaacc
     8221 aaaatactcc agcagcacat taccaggagc cgattcgcca aaaccaccca tagccacagt
     8281 gcgaccatct aaacctacgt atttgtacca gtagtcttca tgggccgctt cgatggcaac
     8341 gcgagcagtg actgccaagg gtaacaccgc ttctttat
//
LOCUS       DAOQGS010000121         8358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4047_length_8358_cov_4.970974, whole genome shotgun sequence.
ACCESSION   DAOQGS010000121 DAOQGS010000000
VERSION     DAOQGS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4047_length_8358_cov_4.970974"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /locus_tag="PKZ52_06280"
     CDS             complement(<1..2179)
                     /locus_tag="PKZ52_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNG59413.1"
                     /translation="MRSAIRFITTLFWMTIAAFSSIALSLIGIYLYLSPGLPSIETLK
                     DTRLQTPLRVYSSDNKLIGEFGEKRRMPIKFSDIPHSYIDALLSAEDDQFFTHKGVSI
                     KALLRASSQLAMSGDIHSGGSTITMQVARNFFLSNKQTFKRKFNEILLALRIERELSK
                     EEILELYVNVIFLGNRAYGIQAAAQVYFGKSLNELSLAELATLAGIPKAPSTINPIAN
                     PNRARERRDWILGRMHELGKIDAQTMELAQREAVKAEFHGANIEFDAPYIAEMARREA
                     VNLFGNKAYTEGYRVYTTISSRFQTFAQEAVINGLFDYDNRHGYRGPEQSFAAKNATA
                     AQKLGPPDANTLANWLGALEKIPTLAHCLPAVVTLVGETKIKATLAEGKEITLGWEQG
                     LSKAAPYLNENAVGAYPKSAKELFKSGDVIRVLPKDDGYELTQIPSAQAAFVAIDSNT
                     GAVLALVGGIDFAQSSFNRATQAKRLAGSNFKPFFYTAALENGYTPATIINDAPIVFE
                     TDNPDEAWRPENSGGKFMGPIPLRKALYLSRNLVSIRILQSLGLDKALEHIDRFGIDK
                     NKVPRNLSIALGTHAMTPLEVVQGYSVFANGGYKVEPYFVDRVEDADGNLLHQTPRYQ
                     VCGDCDGQKLGKSTKPAPRVIDEQTAFIMDSILKDVVRKGTATAAKKLNRNDIAGKTG
                     TTNGPTDVWFSGYGGNITGTAWIGFDQNTNLGRKEFGGTAALPI"
     gene            2331..3407
                     /locus_tag="PKZ52_06285"
     CDS             2331..3407
                     /locus_tag="PKZ52_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNG59414.1"
                     /translation="MSFLSFFEKKTKPMLGLDISSTTIKLLELSRQGDSFRVENYSVR
                     PLPPNAVVEKNINDPEAVAQVIKATITQCRTKVKDCAVAVAGSSVITKLIEMPSGLSE
                     DAMELQISIEADQYIPFPLDEVALDFDVQGPSARNPNQVEVLLAACRRENVEARSTVL
                     QLADLRPRVVDVEAYTMERAFRLIREQLDDQQDQVVAIVDIGSTMTTLSVLVDGKTIY
                     TREQLFGGRQLTEEIQRRYGLSEEEAGLAKKQGGLPDDYGPEVLEPFKESVVQQVTRS
                     LQFFFSSTQYNDVDHIVLAGGVSSMRGLAEVIEAKLGTSVTVANPFANMSVASRVNST
                     ALANDAPALMIATGLALRSFDLDG"
     gene            3400..3999
                     /locus_tag="PKZ52_06290"
     CDS             3400..3999
                     /locus_tag="PKZ52_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNG59415.1"
                     /translation="MAKINLLPWREEYRQEKKKEFITQMFGFCLLVGLCSYVWITSVD
                     SAIDNQKQRNKLLEDEIVLLDAQVKEIQELKKKRSELIARMKVIQDLQGTRPTVVRFF
                     DELVRAIPDGVFLTSLVKNGEMLSIDGITESANRVSAFMRQLDDSPWFAEPNLKSVTN
                     DPKFGEQAAKFSLQLKAVLPAEKDEKDVKDAKPKGGGNG"
     gene            3992..4609
                     /locus_tag="PKZ52_06295"
     CDS             3992..4609
                     /locus_tag="PKZ52_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNG59416.1"
                     /translation="MADLREYLEQLKSIEPSEINWERVGVWPWPARAFLAVFAGGAIV
                     AAAYFFVVKDKQLQLEDEVRKEVSLKETFQKKAFEASNLEKYRQQIAEMEESFEALKK
                     QLPNDTEVPGLLEDIDEKGVGSRLVIDSITLQPEKPAEFYVELPIDIKVTGGYHEFGS
                     FVSGIAGMPRIVTLHNFSISRKKDSSSALVMEIQAKTYRYRNQGE"
     gene            4612..5145
                     /locus_tag="PKZ52_06300"
     CDS             4612..5145
                     /locus_tag="PKZ52_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNG59417.1"
                     /translation="MSERIIRLVAVAMSVAGLSGCFFQDDHADLKAYIAEVRARPQGT
                     IEPLPPVRTYDAFIYGATALRSPFDQPVEVKAVVGIRNPDIKPDNSREKEFLESFNLD
                     ELAMVGMMEQKGSLWALMKDGVGGIHRVTLGNYMGKNHGKIISVTADQLDLLEIVSDG
                     LGGWVQRPRTIKLSEKE"
     gene            5151..7241
                     /gene="pilQ"
                     /locus_tag="PKZ52_06305"
     CDS             5151..7241
                     /gene="pilQ"
                     /locus_tag="PKZ52_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNG59418.1"
                     /translation="MSSYYKNIATFIGGIVLASTALASQLTNVKFAEAPGDKVEIRLT
                     FDSATEAPKGYTIEQPARIVLDFPNVDNSLAQKKFPMGVGNVSSAVVVSGGNRTRLIV
                     NMNALSPYVTRQEGNDVVLEVGSASVKGESKVKAQPSLVPQGIGKAKNNFDKATSAIT
                     NIDFRRGDAGEGKVIINLTNPGVNVDVEEVAGGIEVSFMQTDLPAELRRKLDVADFAT
                     PVKIVGANTNNLRAVINIAASGEYDYLAYQADNQYVISVKPLTKAQLAEKKEKFAFTG
                     EKLSLNFQNIEVRSVLQLIADFTDLNLVASDTVKGSITLRLDNVPWDQALELVLKTKG
                     LDKRQVGNVLMVAPAAEIAERERQELATQKQLQELAPLRTEYIRVRYAKARALFELFM
                     PKGQGSGGSGGSGGSQRNSTTSILSDRGSAIVDERTNSIILTDTEDKILEFRKVIDQV
                     DIPIRQVMIEARIVIANNEFRKELGTRISTNLTSTTQQGNTNRLRGSIFNGVDQATNL
                     NVSGPFGAMTWTVIGKNINLGLELSALEDSGFGEIVSQPKVITGDKQQASIENGTQIP
                     YQEASASGATTTAFKEAVLKLDVTPQITPDDNIIMTLKINQDSLGKVVAQSNVPTIDV
                     TELNTQVLVADGQTVVLGGIFQTNKVKGVSKVPLLGDVPLVGRLFRRNKDSDTKRELI
                     IFITPRIMSPTLTN"
     gene            7248..7757
                     /locus_tag="PKZ52_06310"
     CDS             7248..7757
                     /locus_tag="PKZ52_06310"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNG59419.1"
                     /translation="MHNIVLVGPMGVGKSTVGRLLSSRLGFQFLDTDTLIEERCGANI
                     PWIFDVEGEAGFRQREYQALLELQGRSSTVVATGGGIVVTPQNIPLLKSLGRVFFLTA
                     SIDQLVARTSRDKKRPLLQVENPRKRIEELIAARDPLYRAVADFVITTDVKGTKGVVG
                     QIMDIIRQA"
BASE COUNT         2082 a         1915 c         2214 g         2147 t
ORIGIN      
        1 aaataggcag cgccgcagta ccgccgaatt ctttgcggcc tagattggta ttttggtcaa
       61 agcctatcca ggcggtgccg gtaatattgc cgccataacc ggagaaccac acatcggtag
      121 ggccgttggt ggtgccggtt ttacctgcaa tatcgttgcg gttaagtttt ttagcggcag
      181 tggcggtgcc cttacgcacc acgtccttca aaatagaatc catgataaag gccgtttgct
      241 catcgataac cctaggggca ggtttggtag atttgccgag cttctggcca tcgcaatcgc
      301 cacacacctg gtaacggggc gtttggtgta ggaggttgcc atccgcatcc tcaacacggt
      361 ccacaaaata gggctcaacc ttatagccac cgttggcgaa cacgctgtaa ccctgcacca
      421 cttctaaggg cgtcatggca tgggtgccga gggcgatgga aagattgcgg ggcactttat
      481 ttttgtcgat accaaagcgg tcgatatgct ccagggcttt atccagcccc aggctctgca
      541 aaatgcggat ggagactaaa ttgcgggata aatacagcgc cttgcgcagc ggaatcggcc
      601 ccataaattt accgcccgag ttctccggcc gccacgcttc gtcggggttg tcggtttcaa
      661 acacgatggg cgcatcattg ataatggttg ccggggtgta accattttcc agcgcagctg
      721 tataaaagaa gggtttgaag ttggagcccg ccaagcgttt agcctgggtg gcgcggttaa
      781 aactgctctg ggcaaaatca atcccgccca ccaaggccag cacggctcca gtgttggaat
      841 ctatggccac aaaagcggcc tgggcactgg ggatttgtgt aagttcatag ccatcatcct
      901 tgggcaatac gcgaatcaca tcgccgcttt taaataactc tttggccgat ttagggtagg
      961 cgcccacggc gttttcatta aggtagggcg ccgcttttga taggccctgc tcccagccga
     1021 gggtaatttc tttgccctcg gcgagggtgg ctttaatttt ggtttctccc actagggtaa
     1081 ctacggcggg caggcaatgg gctagggtgg gaattttttc cagtgccccc agccaattgg
     1141 ccagcgtatt agcatccggg gggccgagct tttgcgcagc ggtggcattt ttcgcagcaa
     1201 atgactgctc cggcccccgg tagccgtggc gattatcgta gtcaaacagg ccatttatca
     1261 cagcttcttg cgcaaaggtt tgaaaacggc tgctgatcgt tgtataaact cgatagcctt
     1321 cggtgtacgc cttattgcca aacagattta ccgcttcccg ccgcgccatt tctgctatgt
     1381 agggtgcgtc aaactcgatg ttggcgccgt ggaattcagc cttaacggct tccctctgcg
     1441 ccagttccat ggtttgtgcg tcgatttttc ccagttcgtg catacggcct aggatccagt
     1501 cccgccgctc gcgggcgcgg ttggggttgg caatggggtt tatagttgaa ggggccttag
     1561 gtatacctgc gagcgtcgcc aattcggcca ggctaagctc gttgagtgat ttaccgaaat
     1621 acacctgcgc tgcggcctgt atcccgtagg cgcggttacc aagaaaaata acattaacgt
     1681 acagctcaag aatttcctct ttgctcagtt cgcgctcaat gcgcaaggcc aagagaatct
     1741 cgttaaattt gcgtttaaac gtttgtttat tactgagaaa gaagttgcgc gcaacttgca
     1801 ttgtgatggt tgagccgccg gagtgaatat cgccggacat ggctaactgc gacgatgccc
     1861 gcagcagcgc tttaatagac acgcccttat gggtaaagaa ttggtcgtcc tccgctgaga
     1921 gcaaagcatc tatatagctg tgcggtatgt ctgagaactt aatcggcatc cggcgttttt
     1981 cgccaaactc gcctataagt ttattatcgc tggagtagac ccgcaggggc gtttgtaaac
     2041 gagtgtcctt aagggtttcg atggaaggta ggccggggct taagtacaag taaatgccga
     2101 ttaggcttag tgctatagag ctaaaagctg ctatagtcat ccagaataaa gtagtaataa
     2161 atctaatggc tgagcgcatt cgaggcagtc ttaaggtttt gtgaggtggg ccttatcggg
     2221 agcggcatta tagtggattt tcacacttta cctacgagat agcgtgttgc ggctctaagt
     2281 taaagtgcta taacatgaac ctgagctaag tagcggataa agatagaaaa atgagctttc
     2341 taagtttttt cgaaaaaaag acaaaaccca tgcttggcct ggacatcagc tccactacca
     2401 tcaagctgtt ggaattaagt cgccaggggg attccttccg cgtcgagaat tattccgtgc
     2461 ggcccctgcc ccccaatgcg gtggtggaaa aaaacatcaa tgacccggag gcggtcgcgc
     2521 aggttatcaa agctaccatc acccaatgcc gcaccaaagt taaagactgc gccgtagcgg
     2581 tggcaggttc ttcggtgatc actaagttaa ttgaaatgcc cagcggccta tctgaagatg
     2641 ccatggagtt acaaatctcc attgaggccg accaatacat cccattcccc ctcgatgaag
     2701 ttgccttgga ttttgatgtg cagggccctt ccgcgcgcaa ccccaatcaa gtcgaagtgc
     2761 tgctggcggc ctgtcgccgt gaaaatgtcg aagctcgctc cacggtgttg caattggctg
     2821 atttgcgccc cagggttgtg gacgttgagg cctacaccat ggagcgcgcc tttcgtttaa
     2881 tccgtgaaca gttggatgat cagcaggatc aggtggttgc catagtggat ataggttcca
     2941 ccatgaccac tctgtcggtg ttggtggatg gcaaaacgat ttatacccgt gagcagttgt
     3001 tcggtggcag gcagttaacc gaagaaattc agcgccgtta cggcctctca gaagaggaag
     3061 ccgggcttgc taagaaacag ggtggtctgc ccgacgatta cggccctgaa gtgcttgagc
     3121 cctttaaaga gtccgtcgtt cagcaggtga cccgctcgtt acaatttttc ttctcctcca
     3181 cccaatacaa cgatgtggat cacatagtgt tggccggtgg tgtctcctcc atgcgcggcc
     3241 ttgctgaggt gatagaggcc aaactgggca cctcggtcac ggtggccaat ccatttgcta
     3301 acatgtccgt ggcttcccgg gttaattcca cagcgctcgc caatgatgcc cccgccctaa
     3361 tgatcgcaac cggtcttgcc ctgaggagtt ttgatctcga tggctaaaat aaacctattg
     3421 ccctggcggg aagagtatcg ccaggagaag aaaaaagaat ttatcaccca gatgtttggg
     3481 ttttgtctat tggttggtct ctgctcttat gtgtggatta ccagtgttga cagcgccatc
     3541 gacaatcaaa aacagcgcaa caagctgctc gaggatgaaa ttgttctgct ggacgctcag
     3601 gttaaagaaa ttcaagaatt aaagaaaaag cgcagcgaat tgatcgcgcg catgaaggtg
     3661 attcaggatt tacagggcac acggccaacg gttgtgcgct tcttcgatga gttggtgcgc
     3721 gccattcccg acggtgtttt cctaacttct ctagtcaaga atggagaaat gttgtccatc
     3781 gacggcatta ccgaatcggc caaccgcgtg tcggccttta tgcgtcagtt agacgattca
     3841 ccttggtttg ccgagcccaa tcttaagtct gtcaccaatg accccaagtt tggtgagcag
     3901 gctgccaagt tctctttgca gcttaaagct gttctccccg ctgaaaaaga tgaaaaagac
     3961 gtgaaagacg caaaaccaaa agggggcggc aatggctgat cttcgcgagt acctcgaaca
     4021 gcttaaatcc attgagccat cggaaattaa ttgggagcgc gtaggcgttt ggccctggcc
     4081 cgcgcgtgcc tttttggccg tttttgccgg tggtgccatt gtcgcagccg cttatttttt
     4141 cgtggtaaag gataagcagc tgcagttaga agatgaggtg cgcaaagagg taagtttgaa
     4201 agaaaccttt cagaaaaaag cctttgaagc ctctaaccta gaaaaatacc gccagcaaat
     4261 cgctgaaatg gaagagagct ttgaggcgct gaaaaaacaa ttgcccaatg atacagaagt
     4321 gccgggtctg ctcgaggaca tagatgagaa aggtgttggc agccgtttgg tgattgacag
     4381 cattacatta caaccggaaa agcctgcgga attttacgtt gaattgccaa ttgatattaa
     4441 agtgaccggg ggttatcacg aattcggatc gtttgtgagc ggtatcgcag gtatgcctcg
     4501 aattgtaact ttgcataatt tttcaatatc gcggaaaaag gattccagca gtgcgttggt
     4561 catggaaatt caagctaaga cctatcgtta tcgcaatcag ggtgaatagt tatgtctgag
     4621 agaattattc gtctagttgc ggtggcaatg agtgtggcag gcttaagcgg gtgttttttt
     4681 caggatgatc acgccgattt aaaagcttat atagcggaag ttagggcgcg cccccagggt
     4741 actatcgagc cgctgccgcc agtgcgcacc tacgatgcat ttatatatgg tgctaccgct
     4801 ttgcgcagtc cgtttgatca accggttgag gttaaagctg ttgtggggat acgcaatccc
     4861 gatatcaaac ctgacaacag tcgcgaaaaa gaatttttag agtcattcaa ccttgatgag
     4921 cttgcgatgg ttggtatgat ggagcagaag ggaagcctat gggctttaat gaaggatggg
     4981 gtaggaggaa tccatagggt gacgctgggt aactatatgg gtaagaatca cgggaaaata
     5041 atctcagtga cggcagatca attggatctg ctggaaattg tttccgatgg tttgggtggc
     5101 tgggtgcagc gtccgcgaac tattaaatta tctgagaagg aataattggc atgtcgtcct
     5161 attataaaaa tatagccacc ttcatcggcg gcatagtgtt ggccagtacc gcgctcgcca
     5221 gccaattaac caatgtcaaa tttgctgaag ctccaggcga caaggtcgaa attcgactga
     5281 cgtttgattc cgccaccgag gcacccaagg gctacaccat cgaacagccc gcacgtatcg
     5341 tgttggattt cccgaatgtg gataactcct tggcccagaa aaaattcccg atgggggttg
     5401 gcaatgtcag tagtgcggta gtggtttccg gcggcaaccg cacccggctg atcgtcaata
     5461 tgaatgccct ctctccttat gtaacccgcc aagaaggtaa tgatgttgta cttgaagtgg
     5521 gcagcgcctc tgtaaaagga gagtcgaaag tgaaagccca accttctttg gtgccgcagg
     5581 gcataggaaa agccaaaaat aatttcgata aagcgacatc agccattact aatatcgatt
     5641 ttcgccgtgg cgacgcgggt gaaggtaagg tcatcatcaa cctgactaat cctggcgtga
     5701 atgtggatgt ggaagaggtt gcaggtggaa ttgaagtaag tttcatgcag actgatttgc
     5761 ctgccgaatt gcgccgcaag ttggacgttg ccgattttgc tacgccagtg aaaatagttg
     5821 gtgctaatac taataacctg cgcgctgtga tcaatattgc cgccagtggc gaatacgatt
     5881 accttgccta ccaagctgac aatcaatatg taattagcgt taagccgctg actaaagcac
     5941 agcttgccga gaaaaaagaa aaatttgcct tcacgggcga aaagctctcg cttaatttcc
     6001 aaaatattga agttcgctca gtattgcagt tgatcgctga ttttactgat ctgaacctgg
     6061 tggccagtga tactgtgaaa gggagtatta ctctgcgctt agataatgtg ccctgggatc
     6121 aggcgctgga gttggtgcta aaaaccaaag gtttggataa gcgtcaggta ggcaatgtat
     6181 tgatggttgc accagcagca gaaattgctg agcgcgaacg gcaagagctg gctacacaaa
     6241 aacaattaca agagttggct ccattgagga cagaatatat tcgtgtgcgt tatgcgaaag
     6301 ctcgcgcgct atttgaattg tttatgccga aaggacaggg tagcggtgga tctggtggta
     6361 gtggaggttc ccagcgtaat tctacgacta gtattctttc tgatcgtggc tcagcaattg
     6421 tggatgagcg caccaattca attatcctaa ctgatactga agataaaatt cttgaatttc
     6481 gtaaggtgat tgatcaggtc gatatcccaa ttcgacaggt aatgatcgag gctaggatcg
     6541 ttatcgcaaa taatgaattt aggaaggagc ttggtacacg tatctccacc aacctgactt
     6601 caactacaca acagggcaat acaaatcgcc tgcgtgggag catattcaat ggtgttgacc
     6661 aagcaactaa tttaaatgtt tccggcccat ttggtgcgat gacttggacc gtaatcggca
     6721 agaatatcaa cttaggtctg gagttgtcgg ccttggaaga ctctggcttc ggtgaaatcg
     6781 tcagccaacc caaggtcatc actggagata agcaacaagc cagcattgag aacggaaccc
     6841 aaattcctta ccaagaggct agcgccagcg gtgcaacgac gacagcattt aaagaggctg
     6901 tattaaagtt ggacgtaacc ccccaaatca ctcctgatga caacattatt atgacgctca
     6961 aaattaatca ggattcattg ggcaaggtgg ttgctcagtc aaacgtgccc accattgatg
     7021 tgaccgagtt gaatacacag gttttggttg ccgatggtca aacggtggtg ctgggtggca
     7081 tattccaaac gaacaaggtt aaaggtgttt ccaaagtgcc tttgctgggg gatgtgccgc
     7141 tggttgggcg cttattccgt cgtaataaag acagcgatac caagcgtgag ttgattatct
     7201 ttattactcc tcgaatcatg tcgccgactt tgactaacta gttcggcgtg cacaatatag
     7261 ttcttgttgg cccaatgggg gtggggaagt cgactgttgg tcggcttctg tcatcccgat
     7321 tgggttttca gtttttagat actgataccc ttattgaaga gcgttgcggg gcgaatatcc
     7381 cctggatttt tgatgtggag ggcgaagcag gatttcgtca gcgagaatat caagccttgt
     7441 tggaattgca ggggcgctcg tccacagtgg tggcaaccgg tggtggaata gtggtgacac
     7501 cccagaatat tccgctctta aaatcgctgg gcagggtatt tttcctcacg gcttcgattg
     7561 atcagctggt ggctcgcacc agtcgcgata aaaaacgccc gctcctgcag gtggaaaacc
     7621 cacgcaagcg cattgaagaa ttaattgccg cccgtgatcc gctgtaccgc gcagtggcag
     7681 attttgtgat tactacggat gtgaaaggca ccaaaggcgt ggtggggcaa attatggata
     7741 ttatcaggca ggcctgaata gctttcccgt tctgtccgca cggtaatttc ctctcttacg
     7801 agttctcgcc tttttattga taaatcacaa tgacgatttg ttatcaacgc ctggcccttc
     7861 caactgcaat taagtgtttt ctgaagcagg atcgctcgtt tgtccgcact taagtttccc
     7921 ttctaatttt tgctacgagt tcggatgcgt ttccaggtaa agatgtgaaa gtcgtgccgc
     7981 catcagaggc agtggtcggc gagctgcaaa ataaagcact tttgcttggt acttatggat
     8041 ctgactaaat cgagggggcg cccatttgcc aactctttta tatagctgaa taagtagcca
     8101 ttgctaatta tcctaaaagc cgcagttgaa ctctaatccg gccgcctaat gcttgaaact
     8161 acacgctttt ttgtcaaaat ttgactcacc ttagtagcga ctactgattt cgccaaattt
     8221 tgccaaaaaa tcgctcaaat tcagcgcctt aggcaccctc gctacgagtt caactgcggc
     8281 ttttgggatt atttaggtgc gtcaattatg cggccagtct gcgaaagact ggtttttcag
     8341 ttatgggctg gcgataat
//
LOCUS       DAOQGS010000122         8344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4056_length_8344_cov_4.028954, whole genome shotgun sequence.
ACCESSION   DAOQGS010000122 DAOQGS010000000
VERSION     DAOQGS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4056_length_8344_cov_4.028954"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKZ52_06315"
     CDS             complement(<1..375)
                     /locus_tag="PKZ52_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59420.1"
                     /translation="MTTTQVPITHTLKTLSACILVIAILKADALSRYWQQQWRVALPL
                     AGNLTQPTWFNQLWSNYFSTTELFITTLNERLKRAGQALSTDPIAPTPAVTPAPISTA
                     QCEDTPKEKAIDEPAKALSANKS"
     gene            complement(377..1777)
                     /locus_tag="PKZ52_06320"
     CDS             complement(377..1777)
                     /locus_tag="PKZ52_06320"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNG59421.1"
                     /translation="MHYLSPEFAFVFLAFLWFYWLLGVGSGPRAQNQLLLLASYGFYA
                     SFDWRFLVLLISFSLIILGLAGLVVRPDGSRRRWPVSLGIVLALCNLGLFKYYNFFRE
                     SLVAALGTSIENSSLPLLDILLPVGISFYTFCGIAYLIEVGRGNQQPAPWLEGCLLLA
                     FFPTLLAGPICRPKELLPQLSQEQARTIIEPERIGLLILSALIKKIWLASWLAEQFVK
                     PVFADPSAFHALDLTAAIIAYAWQIFFDFSGYTDIVTACALLLGIKVPVNFLQPYLAT
                     SLSDFWQRWHISLSSWIRDYVYIPLGGSRGNWWRTQLNALLAMVISGIWHGANLTFVI
                     WGLWHGAGLIAQNLWRRLNKNTLPRGLAQTLTFVFVCIGWVFFRADDVPRAVEFLQGF
                     GQWRIPTQLNYLGLFAACAIYFYCSRTSQVFVDYLQCKLANVHWSLQAAAITTCALFI
                     IQLAPSGMPGFIYFGF"
     gene            complement(2070..3698)
                     /gene="nadB"
                     /locus_tag="PKZ52_06325"
     CDS             complement(2070..3698)
                     /gene="nadB"
                     /locus_tag="PKZ52_06325"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNG59422.1"
                     /translation="MSEPTTSHFYSDVLVIGSGAAGLTLALKLAQICKVSVLSKGKLT
                     EGSTWHAQGGIAAVLDDDDSIESHVADTLVAGAGLCHPQAVRFTVSRSKAAIEWLVGL
                     GVDFTRSDNSHYHLTQEGGHSHRRIIHSADATGKAVHSTLLEQALAHPNIKLLENHVA
                     LDLIREPDLASGKMRCVGAYVYDRTLDRVVTHSAKTSILATGGASKVYLYSSNPDSAS
                     GDGIAMAWRAGCRVANMEFNQFHPTCLYHPDAKSFLLTEALRGEGAFLKLPNGERFMQ
                     NFHPMAELAPRDVVARAIDHEMKRLGSDYVLLDISHKPADFIQHHFPTVWSNCLQFGF
                     DITKDPIPVVPAAHYTCGGVVVNCHGQTDLKNLFAIGETSFTGLHGANRMASNSLLEC
                     IVYAHSAAEKIEQDLANIPAPSAIKEWDESRVTNSDEDVVISHNWDELRRFMWDYVGI
                     VRTHKRLERATNRIRLLQKEIQDYYANYKVGSDLIELRNLAMVAELIIRSAMERKESR
                     GLHFSLDFPEVSPIAKDTILVPTNFADQELFVHN"
     gene            4058..4639
                     /gene="rpoE"
                     /locus_tag="PKZ52_06330"
     CDS             4058..4639
                     /gene="rpoE"
                     /locus_tag="PKZ52_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNG59423.1"
                     /translation="MLEPSAPDADQPLVSRVQQGDKKAFDLLVIKYQHRIMAVVSRFI
                     RDHAEVQDVAQETFIKAYRALPNFRGESAFYTWLYRIAINTAKNFLVSRGRRPPAMDI
                     DAEEAEFLTDSENMRDIENPENNLMRDQLDAVITKAIADLPRDLRTAITLREIEGMSY
                     EEIAEAMGCPIGTVRSRIFRAREAIDRQMAPLL"
     gene            4748..5530
                     /locus_tag="PKZ52_06335"
     CDS             4748..5530
                     /locus_tag="PKZ52_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNG59424.1"
                     /translation="MTSSDEMLKESLSALMDNQASDLELRRILKQLPEDEALGATWRR
                     YQLASTLLQRKETAFLHLDISTAISAAIASEPALGPINQHAVAAVKTAPAWLRWGGKS
                     AVAASVAVAVLIGAQQFSNPSLAPLAVTPATPASGLAAKPTEPDNLAGIAANLPEASL
                     PESGQVQSAPDGFALPVPMARTVSSNAERVQPLQVQPVSLNQTDWRNDPEVQAQLNQM
                     LLDHASRSAVHGSFGLLPFTRVANGPTDVSLNTSAEPVAGKR"
     gene            5530..6522
                     /locus_tag="PKZ52_06340"
     CDS             5530..6522
                     /locus_tag="PKZ52_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNG59425.1"
                     /translation="MVKTIRTLLKPPPVWLGLFFALVVLPFATVSARALEPLATVDDL
                     LRQLSQSLQERGFRGHLTYEYGGQMDSLAIVHGLNNGVEQERILHLSGPAREATRQGR
                     LASCINAGSFLLRGGLASNAQGVSASINQNYQFVLRGEERIAGRQAAIIQMVPKDALR
                     YGVTLALDKATGLPLMWLIQNGQRKILERMQFVDIQLDPKFTDNDFKAEGLAVGIGVN
                     KEQCFSAPVQPSQWRFHWLPPGFVLAQADREQSGEYLTFTDGLAVFSVRVAPLSSASQ
                     RRVGAALRGATLAMVTAFNDGDNHYQMSLTGEIPPETAQAVLASIGRVPESAQP"
     gene            6669..>8344
                     /gene="lepA"
                     /locus_tag="PKZ52_06345"
     CDS             6669..>8344
                     /gene="lepA"
                     /locus_tag="PKZ52_06345"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNG59426.1"
                     /translation="MSTLSHIRNFSIIAHIDHGKSTLADRFIQHCGGLSAREMEAQVL
                     DSMDLERERGITIKAQSVTLNYTSSDGKTYQLNFIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGVEAQSVANCYTAIEQGLEVIPVLNKMDLPQAEPDRVAEEIENIIGIDATD
                     AVRCSAKSGLGIEDVLERLIERIPPPEGDPEAELQALIIDSWFDNYLGVVSLVRVKHG
                     TLRVKDKIMSKTIGKANVVDSVGVFTPKRHETGVLKAGEVGFVVAGIKDIHGAPVGDT
                     LIHANCPHVEALPGFKRVQPQVYAGLFPVSSDDFEAFRDALSKLTLNDASLFYEPESS
                     DALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVFEVEQTNGEIVYVDNPSK
                     LPEISNIADMREPIVQANILVPHAYLGAVITLCIEKRGVQRDLQYMGSQVSLVYELPM
                     NEVVLDFFDKLKSVSRGYASLDYSFLRFQSARLVRLDVLINGEKVDALALIVHRDTAQ
                     YKGRALTEKMQELIPRQMFDVAIQAAIGGQIVARSTVKALRKNVLAKCYGG"
BASE COUNT         1961 a         2044 c         2298 g         2041 t
ORIGIN      
        1 ggatttattg gcgctgagag ctttagccgg ttcgtcaatt gctttttctt taggtgtatc
       61 ttcacactgg gcagtgctaa taggtgcggg ggtaactgcc ggtgtcggtg caatgggatc
      121 ggtgctgagt gcttggcctg cgcgctttaa gcgctcgttg agggtcgtga taaacaattc
      181 tgtggtgctg aaatagttgc tccacagttg gttaaaccag gtgggttggg ttaggttacc
      241 ggccagtggg agtgcaacgc gccattgttg ttgccaataa cggctgagtg catccgcttt
      301 gagtatagcg ataaccaata tacaggctga tagtgttttt aacgtgtgtg taataggtac
      361 ttgtgtggtt gtcatcctag aacccgaaat aaataaaacc aggcataccg cttggcgcta
      421 attgaatgat aaaaagtgcg caggttgtga tggctgccgc ttgtaggctc cagtggacat
      481 ttgccaattt gcactgtaaa taatctacaa aaacttggct tgtgcgggag caataaaaat
      541 aaatcgcgca ggctgcaaat aatcctaagt aatttaattg cgtgggaatg cgccactggc
      601 caaagccttg caaaaattct actgcacgag gtacatcgtc cgcgcggaaa aatacccatc
      661 ctatgcatac aaaaacaaaa gtgagtgttt gggctaagcc tcttggcaga gtgtttttat
      721 tgaggcgccg ccagagattt tgcgcgatta aaccggcgcc gtgccacagc ccccaaatga
      781 caaaggttaa atttgcgccg tgccaaatgc ctgaaattac catggctaat agggcattaa
      841 gctgcgtgcg ccaccagttg ccgcggctgc cgcctagcgg tatatacaca taatcgcgaa
      901 tccagctgga taaactgata tgccagcgct gccaaaaatc actcaggctg gttgctaaat
      961 agggttggag aaaattaacc ggaactttta tgcccagcag caaggcgcag gcagtgacga
     1021 tatcggtgta gccggaaaaa tcaaaaaata tttgccaggc ataggcgatt attgcggcgg
     1081 ttaaatctag ggcgtgaaag gcgctcggat ctgcaaaaac aggttttaca aactgctcgg
     1141 caagccagct ggccagccaa atctttttta ttaaggcgct cagtattaat aggccaatgc
     1201 gttctggctc aataattgtg cgcgcctgtt cttggctgag ttgtggtaat agctctttgg
     1261 gccgacaaat aggccctgcc agcaaggtgg ggaaaaacgc caataataaa caaccttcca
     1321 gccagggggc gggctgttga ttgccgcggc ccacttcaat taaataggcg atgccacaaa
     1381 aggtataaaa cgaaataccg acgggtaata aaatatccag taggggtaaa ctggagtttt
     1441 caatgctggt accgagcgcc gccaccaggc tttcgcggaa aaaattatag tatttgaaga
     1501 ggcctaaatt acacagagct aaaacgatgc ccaggcttac cggccagcgc ctgcggcttc
     1561 catccggtct tactaccaag cctgctaggc ccaaaataat cagtgaaaaa ctgatcaata
     1621 agacgagaaa acgccagtcg aaactggcgt aaaacccgta gctggccagt agcaataatt
     1681 ggttttgcgc gcgggggcca gaacctacgc ccagtagcca gtaaaaccac aagaaggcta
     1741 aaaatacaaa ggcaaattcc ggggacagat aatgcataaa aaacagagcc agccttgggc
     1801 aaaggggcgc attctaacgg atggcgatag taggtgctat ttgtaaaggt actttatcta
     1861 gggaaccttt gaaaaattac tttactcgcc ctgacggcgt tgaatttggc aaaaaatact
     1921 catttactat gtgtaaaccc cgcttttttt gccgaattcg tctagccgga gcttcgccgc
     1981 ccacattttt cagaggttcc ctagcctgca gtgggcgtat ccctcgccag ggtaatgggt
     2041 gtgcagacaa aattgcgcag gtaagggcgc tagttgtgga caaaaagctc ctgatcggcg
     2101 aagttggtgg gcactaaaat ggtgtctttg gcgatgggtg acacctcggg gaaatccagg
     2161 gaaaaatgca agccgcggct ttctttgcgt tccatcgcgg agcgaataat cagttccgcc
     2221 accatggcta agttgcgcag ttcgattaaa tcactgccca ctttgtaatt ggcataataa
     2281 tcctgaattt ctttttgcag caggcgaatg cggttggtgg cgcgctctaa gcgcttgtgg
     2341 gtgcgcacta tgcccacgta gtcccacata aaacgccgca attcatccca gttgtgggag
     2401 atcaccacgt cctcatcaga attggttaca cgggattcat cccattcttt tatggccgat
     2461 ggcgcgggta tgttggctaa atcctgttcg attttttccg ccgccgagtg ggcgtaaacg
     2521 atacattcca gcagtgaatt actggccatt cgattggcgc cgtgtaaacc ggtgaaggag
     2581 gtttcgccta tggcgaagag attttttaaa tccgtctggc cgtggcaatt aactaccacg
     2641 ccgccgcagg tgtagtgcgc tgcgggcact accggaattg ggtctttggt gatatcaaag
     2701 ccaaattgca ggcagttgct ccaaacggtg gggaagtggt gctgaataaa atcggccggt
     2761 ttatggctga tatcgagtaa aacataatca ctgcccagcc gcttcatttc atggtcgatg
     2821 gcgcgggcca ccacatcccg tggcgcaagc tctgccatgg ggtggaagtt ctgcataaac
     2881 cgctcgccgt taggcaattt taaaaaggcg ccttccccgc gcagggcttc ggtgagcaga
     2941 aatgatttgg catcgggatg atacaaacag gtggggtgaa attggttaaa ttccatattg
     3001 gccacgcggc agcccgctct ccaggccatg gcgatgccat cgccactggc gctatcgggg
     3061 ttactgctat agaggtagac cttgcttgcg ccaccggtgg cgaggatgct ggttttggcg
     3121 ctgtgggtga ccacgcgatc caaggttcgg tcatagacat aggcgcccac acagcgcatt
     3181 ttgccgctgg ctaaatccgg ctcgcggatt aagtccagtg caacgtggtt ttccagtagc
     3241 ttgatattgg ggtgggcgag ggcttgttct aacagcgtag agtgcacggc tttgccagtg
     3301 gcatcagcgc tgtgaatgat gcgccgatgg ctgtggccgc cctcctgagt taaatggtag
     3361 tggctattgt cgctgcgggt gaagtcaacg cctaagccca ctaaccattc aatagccgct
     3421 ttgctgcggg aaactgtaaa ccgtaccgct tgtgggtggc agaggccagc gcccgccacc
     3481 agtgtatcgg ccacgtggga ttcaatactg tcgtcgtcat ccagtacggc tgctatgccg
     3541 ccttgggcgt gccaggtgga accctcggtc aacttgcctt tgctcagtac actaactttg
     3601 cagatttgtg ccagcttcaa tgcgagggtg aggcccgcgg caccgctgcc gatgactaat
     3661 acgtcgctgt aaaaatggga agttgtaggt tcagacataa tcaatcatct agcaaaaaag
     3721 ggagtgcggt atacgcagat agggcatggg cgtatggctg gatagggaat tatcccggcc
     3781 ctgtgctgaa ctttgcaact ggcgatgggt cttcaccgcc tagtgtgtgc agtgccgggc
     3841 gctggaaaaa tccacctggc tattgcccaa tagggggcct tcataaacaa tggcggctgt
     3901 cggatgtgct tgtgcctaaa ttattctggc gatgcatgcc aaactgagtg tatgaatttt
     3961 gtgtgcactg gtttttggtt ttaccccttg atagcgtttt agaaaaaacg aggggaacat
     4021 ttcttacccg gtgttagcac cgtacctgga gcctttaatg cttgaaccat ctgccccaga
     4081 tgcagatcaa cccctcgtca gtcgagtgca gcagggtgat aaaaaagcgt ttgatctttt
     4141 agttattaaa taccaacacc gcattatggc tgtcgtaagc cgttttattc gcgaccacgc
     4201 agaagtgcag gatgtcgccc aggaaacctt tattaaagcc tatcgggcac tgccgaattt
     4261 tcgcggtgaa agtgcctttt atacgtggct ttatcgtatt gccattaata cagctaaaaa
     4321 ctttttggtc tcccgtgggc gcaggccgcc cgctatggat atagatgctg aagaagcgga
     4381 atttctcacg gattctgaga atatgcggga cattgaaaac ccggaaaata atttaatgcg
     4441 cgaccaatta gacgcggtaa ttaccaaagc aattgctgat ttgccgcggg atttgcgcac
     4501 ggcgattacc ttgcgggaaa tcgaaggcat gagctatgaa gaaattgctg aagccatggg
     4561 ctgcccgatc ggtactgtgc gctcacgcat tttccgcgcc cgcgaggcca ttgatcggca
     4621 aatggcgcct ttgttataaa aatccactaa aaacaaaccc gtggttggaa cctagcctga
     4681 aaggctctgt cacaactgca ccttaatcga ctctcccaga gttttgttaa atccacaggt
     4741 tttatctatg acatcatccg atgaaatgct aaaagagtcc ttatccgctt tgatggataa
     4801 ccaagccagt gatcttgagc tacgtagaat tcttaagcaa ttacctgaag atgaggcgct
     4861 gggtgcgacc tggcggcgtt atcaattagc ctccacgctt ttgcagcgca aagagacagc
     4921 gtttttacac ctggatatat ccacggccat ttctgcagcg attgcttccg aaccggcttt
     4981 gggcccaatc aaccagcacg ctgtcgcagc ggttaagaca gcgccggcct ggctgcgctg
     5041 gggcggtaaa tctgcagtgg cggcgtcggt tgcggtggcg gtgttgattg gtgcccagca
     5101 gttttcaaac ccatccttag cacctttggc ggtcaccccc gctacgcctg ccagcggtct
     5161 tgccgcgaaa cccacagagc cagacaactt ggctggcatc gcggccaatt tacctgaggc
     5221 cagtttgcct gagagcggcc aggtgcagtc cgcaccggat ggctttgcgc tgccagtgcc
     5281 tatggcccgc accgtgagca gcaatgctga gcgtgtgcag cctttacagg tgcagccggt
     5341 ttccctgaat caaaccgatt ggcgcaatga tcctgaggtg caggcgcagc tcaatcaaat
     5401 gttgttagat cacgccagcc gctctgcagt ccacggcagt tttggcctgt tgccgtttac
     5461 gcgcgttgcc aacggcccta ctgatgtatc cctcaatacc agtgccgagc cagttgcagg
     5521 caaacgctga tggttaaaac catacggaca ctccttaagc ccccgccggt atggctgggg
     5581 ctttttttcg ccctggttgt tttgcctttt gctaccgtaa gtgcgcgggc cttagagcct
     5641 ttggcgactg tggatgattt actgcgtcag ttgtcgcaat ctctgcaaga gcggggcttt
     5701 cgcggccacc tcacctatga atacggcggg cagatggatt cccttgccat agtgcacggc
     5761 ctaaataatg gtgttgagca agagcgcatc ctgcacttgt ccggccctgc ccgagaggct
     5821 acgcggcagg ggcgcctcgc cagttgtatt aacgctggca gctttttatt gcgcgggggg
     5881 cttgctagca atgctcaggg tgtctcggcg agtattaacc aaaattatca gtttgttttg
     5941 cgcggtgaag agcgcattgc cggtcggcaa gccgccatca ttcaaatggt gcccaaagac
     6001 gctctgcgct atggcgtgac cctcgcgttg gataaagcca ccggcttacc tctgatgtgg
     6061 ttaatacaaa atggtcagcg caaaattctt gagcgcatgc agtttgtgga tattcagctg
     6121 gaccctaagt ttactgacaa cgactttaaa gccgagggct tagcggtggg cattggtgtt
     6181 aataaagagc agtgttttag tgctcctgtg caacccagcc aatggcgttt ccattggttg
     6241 ccgccagggt ttgtgttggc ccaggcagat cgcgaacagt ctggcgaata cctcaccttt
     6301 acggatggcc tagcggtatt ttccgtgcgg gtggcaccct tgagtagcgc ctcccaaagg
     6361 cgcgtggggg ccgcactgcg cggtgctacg ctcgccatgg tgaccgcttt taacgacggt
     6421 gataaccact accaaatgag cctgaccggg gagattcccc cggaaacagc ccaggcggtg
     6481 ctggcctcta taggcagagt gccagaatcg gcgcagccct aatgtctacg ggcgctaatg
     6541 gatacggggc cctaatggat acagggcata ggcaggcggc cttacctggc ctgggcaaat
     6601 agagtagact tgccgccttt ttgcgcccca tgggccttcg gtttcattct ttattaggca
     6661 gtttattcgt gtcgaccctc agccacattc gcaacttttc tatcattgcc catattgacc
     6721 acggtaaatc cacactggcg gatcgcttta tccaacattg tggcggcctc tccgcccgtg
     6781 agatggaggc gcaggtgctc gactctatgg acctggagcg ggagcgggga atcaccatca
     6841 aggcccagag tgtaaccctc aattacacct ccagcgacgg aaaaacctat cagcttaatt
     6901 ttatcgacac tcctggccac gtggactttt cctacgaggt atcgcgctca ctggccgcct
     6961 gcgaaggggc gttgttggtg gtggatgcgg cccagggggt ggaagcgcag tcagtggcca
     7021 attgttacac cgccatagag cagggcctgg aagtgattcc ggtgctcaac aaaatggatc
     7081 ttccccaggc tgagccggat cgcgtggcgg aggaaattga aaatattatc ggcatcgacg
     7141 ccactgatgc cgtgcgctgt agcgctaagt ctggcctggg tatcgaggat gtattagagc
     7201 gtttaattga gcgcattccg ccaccggagg gtgatcctga ggctgaatta caggcactta
     7261 ttatcgactc ttggtttgat aattacctcg gcgtggtgtc cttggtgcgt gttaaacacg
     7321 gcacattaag ggtcaaagat aaaatcatgt cgaagaccat tggcaaggcc aatgtggtgg
     7381 atagcgtggg cgtctttacc ccgaagcgcc acgagacggg cgtgcttaaa gccggtgaag
     7441 tgggttttgt ggtcgcaggg attaaggata tccacggcgc gcccgtggga gatacgctga
     7501 ttcacgccaa ttgcccccat gtggaagcgc tgccaggatt taaacgcgtt cagccccagg
     7561 tatacgcagg tctgttcccg gtcagctccg atgacttcga agcctttcgc gatgcattgt
     7621 ctaaattgac cctcaacgat gcctctttat tttacgaacc ggagagttcc gacgcgctgg
     7681 gctttggctt tcgctgtggc tttttgggca tgttgcatat ggagatcatc caggagcgct
     7741 tggagcgcga gtatgaccta gacctgatca ccaccgcacc cacagtggtt tttgaagtgg
     7801 agcagaccaa tggtgaaatt gtctatgttg ataacccatc taaattgccg gaaatctcca
     7861 atatcgccga tatgcgcgaa cctatagtgc aggccaatat actggtgccg cacgcctatc
     7921 tcggcgcggt gattacactc tgtattgaga agcgcggtgt acagcgggat ttacagtaca
     7981 tgggcagcca ggtatccctg gtgtatgagt tgccgatgaa cgaagtggtg ttggattttt
     8041 ttgataaatt aaaatccgta agccgcggtt acgcctccct tgattatagt tttttgcgct
     8101 tccaatccgc gcgtttggtg cgcttggatg tacttatcaa tggtgaaaaa gtcgatgcct
     8161 tagcgttgat tgtgcaccgc gataccgccc agtacaaagg ccgggcatta actgaaaaaa
     8221 tgcaggaatt aattccgcgg caaatgttcg atgtggccat tcaagcggct attggcgggc
     8281 aaatagtcgc tcgcagtacg gtaaaagcat tgcgtaaaaa cgttctggct aagtgttatg
     8341 gcgg
//
LOCUS       DAOQGS010000123         8334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4061_length_8334_cov_5.295084, whole genome shotgun sequence.
ACCESSION   DAOQGS010000123 DAOQGS010000000
VERSION     DAOQGS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4061_length_8334_cov_5.295084"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKZ52_06350"
     CDS             complement(<1..419)
                     /locus_tag="PKZ52_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59427.1"
                     /translation="MATLATSWNSLLFTCCLIFLSACSDSPSQPQPQPQAAPQADAGK
                     DQTAGAFNLVHLDGSASKASNGGQLSFQWKQLNTNWVVVDGGLTNAQVSFYIPAAGIP
                     AEGGEFELTVTDTAGNTHRDQVKILPEPCAPSPGDLLT"
     gene            complement(707..1495)
                     /locus_tag="PKZ52_06355"
     CDS             complement(707..1495)
                     /locus_tag="PKZ52_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59428.1"
                     /translation="MSDTSQNTPPPLVTVNPAHQAEDLSLCKGPANIQLNNFAGVVDW
                     INAMPKPLTLACFIASLPRPLTYNATFSRSSVQPAIGKLNPRIFIQYEKLWLSFVPQE
                     AMTAVKDPLTGERTSVWDSDGIQLLEFSLEVDSDLPVPQSIKGELAFPILKNIPRNAP
                     YARIADSSRESTCKACHANETIIGSLDGAPIFRSKMLRSAKQTLMQPDDIFLHYYACD
                     PQINTGPGTENNEWYRCQMLKAFVEHGPIEWQNFRSDITPCIQE"
     gene            complement(1860..3644)
                     /locus_tag="PKZ52_06360"
     CDS             complement(1860..3644)
                     /locus_tag="PKZ52_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG59429.1"
                     /translation="MSTPAPQTLPPLAPPVAHTAKRVVQFARRHWRMALLQVSLAITG
                     TLLMPVFPSVTQWFFDDIIPNKRQDQILNAALLMFGAFFAIELLFYFRTRVNSAFEQI
                     MVFDLRGQLHRKISHMSLNWFDKQSTGDIFTRMADDVPATQRVVLEGIEQGLTAILQI
                     VMALVVMFNTHVALTWVVLIPTPLIAAGGWIYAKLLAPRELQARAATSAMHGMLFDTL
                     AGIRQIKSYVFEEPQQERFNHTSRHLQSIQKKLMAAAALYSPLMTLLGKIGLVLVMLV
                     GAQWCINTQESPLTPGQLLAFVLLVNMLYEPIARLHGVNQMMVSGLASAQRVFAVLDQ
                     DGEEDLHKGMHLEKLHGAIEFKDIAFSYQPQRPIVQGLSISIKPFQTVAFVGATGSGK
                     STIFQLITGFYSADSGAISIDNYPIEQIAKASLRHNIAYVTQDAFLFATDIRQNLLLG
                     KPDASDEELWQALKLACADEFVARLPEGLNSQVGERGNRLSGGERQRLTMARAFLKNA
                     PILLLDEATSAVDNKSEHLIQQALNALKQNRTCLVIAHRLSTVIDADCIYVLRQGEVV
                     AKGTHTELLASSPYYAELSALAFDSPHK"
     gene            4596..5840
                     /locus_tag="PKZ52_06365"
     CDS             4596..5840
                     /locus_tag="PKZ52_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG59430.1"
                     /translation="MPADNQPNNLYYLSASTAGGAYLFLLTGIGLLAAIATYAVFVEI
                     NAWFMQVVFSVVILAATAGGIYCSLLYRRAYVYFLDAANKQQADPKQLDELAKITRNY
                     HQLMQAILPVWQRQTDLAKSQVDQSITDLTTRFSTLYDRLQNAVAASRSTASGMSGEA
                     GITGVIDFAGTALGQLVKTLRHAMGQRDELLAEIVELSKITAELSTMGAEVAAIASQT
                     NLLALNAAIEAARAGEYGRGFAVVADEVRTLSSRSGETGSRIGKRIEQANGALQKTLE
                     RTTAYALEDNKRMGESEASISTVLAKFQASGACILNSTQQLEQESAQVQADIAEVLVS
                     LQFQDRVSQMLGHVIADIEKFSQFIERQKNNLSTGAAIEIIDAQQWMQELNKTYTTLE
                     QTAVHLNKTSQLTPTSSDVTFF"
     gene            5850..6215
                     /locus_tag="PKZ52_06370"
     CDS             5850..6215
                     /locus_tag="PKZ52_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG59431.1"
                     /translation="MSKVILIIDDSASVRQLVSIALRNAGYTVLEACDGKDGLKQLNG
                     QKINLVISDVNMPNMDGITFIKEMKQNPSYKFMPVIMLTTEGAEGKKQEGKEAGAKAW
                     IVKPFRPEQLVQAVSMLIV"
     gene            6438..6743
                     /locus_tag="PKZ52_06375"
     CDS             6438..6743
                     /locus_tag="PKZ52_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNG59432.1"
                     /translation="MKITRKKTKAGHTLCCEGELTIYTVAAAKKILLGNLDEVIDSIA
                     LDLGNVNKLDTAGLQLLLFVKHIMADCNKKLYVKNSNEEIDSIFKEFDLADQFMPEH"
     gene            6745..>8334
                     /locus_tag="PKZ52_06380"
     CDS             6745..>8334
                     /locus_tag="PKZ52_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNG59433.1"
                     /translation="MDTDRFLQTFFSEAEELLISMEAALLRMDEGDQDLETINEIFRA
                     AHTIKGSAGMFGLVDIVGFTHVVENVLDLARDGKIVIADDLLSILLSCRDHIARLVEN
                     AKHNTRPDHECLAQSERLMNALAPWVLDPQTANSTKAAPPVETVIADDIDAQLEAENS
                     RDLWFIAVRFGDDLLRNGMDPLSILRFLNTLGEIVAISINTDRFPPPDEFDPETLYLQ
                     FELYLRSSASLEEIENAFVFVREESHIKIVPPRSNTAAQTDLINAMPKSNQRLGEILV
                     SSHAISPEELEFAIDTQKQQAKEQAPVALLGDILIDTQATAPEVIHAALSRQQAAPTE
                     SRVASQEQRYIRVEAERLDKLINLIGELVTNRQRVDLLAAGLGNTQLTEAVTSIGSFT
                     EQIRGAALTLRMVAIGDTFLRFKRVVRDTAKELGKEIELEIEGAETELDRAMVEKLND
                     PLMHIVRNAMDHGIEPIALRRERKKPDAGTITLKASHGAGNILIEISDDGGGLDLERI
                     RRKAIANNLIDENAQLSRQDLC"
BASE COUNT         2230 a         1870 c         2121 g         2113 t
ORIGIN      
        1 gtcagtaaat ctcccggtga tggtgcacaa ggttctggga ggattttgac ctgatcgcga
       61 tgagtattgc cggcggtatc cgtcacagta agttcaaatt cgccgccttc ggcagggatt
      121 ccggctgctg gaatataaaa acttacttgg gcattggtaa ggccgccatc aaccaccacc
      181 caattagtat ttaattgttt ccattggaag gagagttgcc cgccattact ggccttggag
      241 gcagacccat ctagatgtac taggttgaat gctccagccg tttggtcttt acccgcatct
      301 gcttgggggg cagcctgtgg ctgtggctgt ggctgagaag ggctatctga gcaggcgctt
      361 aaaaaaataa ggcagcaggt aaacaggaga gaattccaag atgtggctaa ggtggccata
      421 gcggggtttc cttattggca agtacaggct taattcaata aaacagggtt taaattctaa
      481 gagtatcaca ggcgagctta tcgacagaag atggtgatgc tcgttcaggg cagtatattg
      541 gcttaataca cgtatttgat ctgcctattc aggacatgcc attataattc caggcgccca
      601 ggatttgcta ttatttgact tgcaagaaaa taggcagagg gaatttgttg cctttaacag
      661 cactaaatct attgcattta ttctcacctg cttgaatgtc tgttgattat tcctgaatac
      721 acggcgttat atcactgcga aaattctgcc attcaatagg cccgtgctcc acaaatgcct
      781 taagcatttg acagcggtac cactcgttat tttccgtgcc tgggccggtg ttgatttgcg
      841 ggtcgcaggc gtaataatgt agaaaaatat cgtccggttg catgagggtt tgtttcgcac
      901 tgcgcagcat tttggagcga aaaatgggtg caccatctaa agagccgata atggtttcgt
      961 tggcgtgaca cgctttacac gtactctccc gagaggagtc agcaatgcgg gcgtagggtg
     1021 cattgcgggg tatatttttg agtataggaa atgccaattc ccctttaatg gattgcggta
     1081 cgggcaagtc ggaatctacc tctaggctaa attctaataa ttgaattcca tcggaatccc
     1141 atacggatgt gcgctcgcct gtgagtggat ctttaaccgc cgtcatcgct tcttgcggca
     1201 caaacgatag ccataatttt tcatattgaa taaaaatacg cgggttgagt ttgcctatag
     1261 caggctgcac actggagcgg ctaaaagtag cattataagt aagtgggcgc ggtaaactgg
     1321 cgataaaaca cgccagggtt aaaggcttgg gcatggcgtt aatccaatcc accaccccgg
     1381 caaagttgtt cagttggata ttcgccgggc ctttacacag gcttaggtct tctgcctggt
     1441 gcgcgggatt aaccgtgact aaaggcggcg gggtattttg gctagtgtca ctcatgcagc
     1501 tgcacaagct gctagcgaat aaaaagatca ataaagattt aagtgtgatt ggcttcataa
     1561 aaactcacga acgggctgcg ccagggtgag taattcttac agataaaagc agccttatct
     1621 taatccgtat caaaatgctg ggtgggaatt atctgcgggc ctcagccaag catgaaattc
     1681 gtgacctagg cctaattatt ttattttcct tcaacattcg tctgtgtaaa cccttcgctc
     1741 tgcgtggttt ttaggctccc atggttaata gagaagccat ggcaaatacc cgttgttaaa
     1801 ccctcgcgca gcaacggggt tgagtaacca tttgcccatg gctgcgcaga agtgagtgat
     1861 tatttgtgtg gcgaatcgaa ggcaagtgct gaaagctctg cataataggg gctgctggcg
     1921 agcaattcgg tgtgggttcc tttggcgact acttcacctt ggcgcagcac ataaatacag
     1981 tcggcatcga ttactgtaga taagcggtgg gcgataacca aacaggtgcg attttgtttt
     2041 aaagcattca gcgcttgctg aattaaatgt tcacttttgt tatccaccgc cgaggtggct
     2101 tcatccaata ataaaatcgg tgcatttttt aaaaaagcgc gggccatggt tagccgctgg
     2161 cgctcgccgc cacttaagcg attgccccgt tcgcccacct ggctattaag tccttctggc
     2221 aaacgggcca caaattcatc ggcgcaggca agtttgagcg cttgccataa ttcttcatca
     2281 ctggcatcag gtttacctaa taataaattt tgccgaatat ccgtagcgaa taaaaaggca
     2341 tcctgggtta cataggcgat attgtgccta aggctggcct tggcgatttg ttcaatgggg
     2401 taattatcaa tgctaatggc gccgctatcg gcggaataaa aaccagtgat taattggaag
     2461 atagtacttt taccgctgcc agtggcgccg acaaaggcca cggtttgaaa aggtttaatg
     2521 ctgatagaga ggccctgcac aatcggccgt tggggctgat aggaaaaggc tatatcttta
     2581 aattcaatgg cgccatgtaa tttctccagg tgcatacctt tgtgtaaatc ctcctcgccg
     2641 tcttgatcta atacagcaaa aacgcgctgg gcggaggcca ggcctgatac catcatttgg
     2701 tttacgccgt gtaggcgcgc aattggttcg tacagcatat tcaccaagag cacaaaggct
     2761 aataactgcc ccggtgttaa agggctttcc tgggtgttaa tgcaccactg ggcacccacc
     2821 agcatcacca gcaccaaacc gattttaccc agcagcgtca tcagcggtga ataaagcgcg
     2881 gctgccgcca ttaatttttt ttgaatactt tgaaggtggc ggctggtgtg attaaagcgc
     2941 tcctgttggg gctcttcaaa tacataactt ttaatttgtc gaatgcccgc taaggtatca
     3001 aacaacatac cgtgcatggc gctggtggcg gcgcgggctt gtaattcccg cggcgctaat
     3061 aatttagcgt aaatccaacc gccagcggcg attaagggtg tgggaattaa taccacccag
     3121 gtgagggcga catgggtatt aaacatcacc accagggcca tcacaatttg caaaatagcc
     3181 gttaaaccct gttctatgcc ttctaaaacg actctttggg tggcggggac atcatcggcc
     3241 atacgggtaa aaatatcgcc ggtgctttgt ttatcaaacc aattgaggga catatggcta
     3301 attttgcgat gcaactggcc gcgcaaatca aacaccatta tttgttcaaa ggcgctgttc
     3361 acccgggtgc ggaaataaaa taataattca atagcaaaaa acgccccgaa cattaaaagg
     3421 gccgcgttga gaatttggtc ctggcgtttg ttgggaataa tatcgtcaaa aaaccactgg
     3481 gtaacgcttg ggaataccgg cattaacagg gtgccggtaa tggccaggct cacctgtaaa
     3541 agcgccatgc gccagtggcg gcgggcaaac tgcacaacgc gtttggcggt gtgggcaacg
     3601 gggggtgcaa gcggggggag tgtctgggga gcaggggtgg acataagcgg ccttaggatc
     3661 aatagtaggc gcaaccatac cgcagaatgt gtaaaaacgc ttgagcccag cccggcaaaa
     3721 cgcaatccct cgttatgggt aggcgcagcg cggcctttat atacggagtt ttactgcatg
     3781 aatccctggc aaacccgagg gagcagcccc gtcaactagc cccccgggtg tagccccgac
     3841 ctctgcaagg cgcgccttcc ggtgcgcaac ctgtacacgc cagccacatg actttgcgat
     3901 tgggtcgggc ataagacacc taagagccac ttcacctcca aaaaattttg ttattgaggc
     3961 aacaggtggt tgagaaggcg ggctaagggg cagggtgtgt ggcgcgtaat cgccgtgcat
     4021 cccgaggcat catcgcttat cgagaatacg ggtttcctac acggggctgt ggcaatagcg
     4081 gggcataagt gcagcatagg agcaggctca tccaagctaa atcgatttta ttctcaataa
     4141 aactctgggg cggaatattt ccgcctacaa aattaatcag aaattctcta actagataag
     4201 tctagtgact aacgataagc ctcatgttgg tgcatcaata tctattttat cagcttgtgg
     4261 ctgcctgaat atttcaccag gctgttatgg aatttcgaca ccaaaatgcc aattcctata
     4321 acaaataatc acaacccacg gcccaccaaa ataatgcggc cggttaatta ggtgaaatgg
     4381 ttcaccggcc tatcagaata tacggctgcg ttttgacaga gccccacgcc agcactatac
     4441 ctttcccatt ggcactcaaa atagtttcct taacgtgtgt cattcggcct cgccattgag
     4501 ttggattcct cttgccccgc ttaatcacca ctatgggtgg cttatttaga ataaggtcgc
     4561 agtcttatac ttgcagaagg aatatcaatt ctattatgcc cgcagataac caaccgaata
     4621 atctctatta cctctcggct agcactgctg ggggggcata tttatttttg ctcacgggga
     4681 taggcctatt ggcggcaatt gccacttacg ccgtattcgt tgaaataaat gcgtggttta
     4741 tgcaggtggt gtttagtgtg gtgattctgg cggctacagc gggcggcatt tattgcagcc
     4801 tgttatatag gcgcgcctat gtatactttt tagatgcggc taataaacag caggccgacc
     4861 ctaaacaatt ggatgagttg gcaaaaatta cccgtaatta ccatcaattg atgcaggcaa
     4921 ttttgccggt ttggcagcgt caaacggatt tagctaagtc gcaggtggat caaagcatta
     4981 ctgatttaac gacgcgtttt tccaccttgt acgatcgctt gcaaaacgcg gttgccgcat
     5041 cacgctcaac agctagcggt atgagtggcg aggcgggcat cacgggcgtt attgactttg
     5101 caggtacagc tctaggtcaa ttagttaaaa cgctacgcca cgccatgggg cagcgggatg
     5161 aattactggc agaaattgtt gagctgtcaa aaatcacggc cgagcttagc accatgggtg
     5221 ctgaagttgc ggctattgca tcgcaaacta atttattagc gcttaatgcc gcaattgaag
     5281 cggcgcgggc cggtgaatat gggcgcggct ttgcggtggt ggccgatgaa gtgcgcacgc
     5341 tttccagccg ctccggtgaa accggctccc gcattggcaa gcgcattgaa caggccaatg
     5401 gcgccttgca aaaaacctta gagcgcacca cggcctatgc ccttgaagac aataaacgca
     5461 tgggggaatc agaggcatcc atcagcacag tgcttgcaaa atttcaggcc tccggtgcgt
     5521 gtattttaaa ttccacccag caattggagc aggagagcgc ccaggtgcaa gcggatatag
     5581 ctgaggtgct agtaagtttg cagtttcaag atagagtcag ccagatgctt ggccatgtca
     5641 tagcggatat tgaaaaattc agccaattta tcgaacgaca aaaaaataat ctctcaactg
     5701 gcgctgccat tgagattatc gacgcacagc aatggatgca ggaattgaat aaaacctata
     5761 ccacgcttga gcaaactgcc gtgcatttaa ataaaaccag ccaattaact ccgaccagtt
     5821 ctgatgtgac gtttttttag gaatgcttta tgtcaaaagt gatattaatt attgatgatt
     5881 ccgccagtgt tcgacaattg gtaagcattg cactgcgcaa tgccgggtat acagtgttag
     5941 aagcctgcga cggtaaagac ggtttaaagc agcttaatgg gcaaaaaatt aacctggtta
     6001 tctcggatgt aaatatgcca aatatggacg gcattacttt tattaaagaa atgaaacaaa
     6061 accccagtta caaatttatg ccagttatta tgctcacaac ggaaggtgcc gagggcaaaa
     6121 aacaagaagg taaagaagcg ggcgcaaaag cctggattgt aaaacccttc cgcccagagc
     6181 agttggtgca ggcggtatct atgttaattg tataagcctg aagattgtct tcctgcgtgg
     6241 gtggtgtgca actggcgtgg gcagacggcc tgcgccaaag ctgaaaaagg cacttatgag
     6301 cgtgcaaaat atgcccgcgt ttggagttaa tcagagcatc tattcagtag atggttgcat
     6361 ataaattatt tagggcttct aaagccattg attaagccgc ctggcgtaat cttcacactt
     6421 tttggcagga gccagccatg aaaataacgc gcaaaaaaac caaagcgggc cacacccttt
     6481 gttgtgaagg tgaattaacc atttataccg tggcagcggc gaaaaaaata ctgttaggaa
     6541 atcttgatga ggtgattgac tcaattgccc ttgatttagg caatgtaaat aaattggaca
     6601 ccgcgggttt gcagctattg ctatttgtca aacacataat ggccgattgc aacaaaaaat
     6661 tatacgttaa aaatagtaat gaagaaatag atagtatttt taaagagttt gacctagctg
     6721 atcagtttat gccggaacac taagatggat acagatcgtt ttttacagac atttttctcg
     6781 gaagctgaag agctgcttat ttccatggag gcagcattat tgcgcatgga cgagggcgat
     6841 caagacctgg aaaccattaa cgaaatattc cgcgcggcac acactataaa aggctctgcg
     6901 ggcatgttcg gcctggtgga tatagtgggc tttacacacg ttgtggaaaa cgtattggac
     6961 ctggcccggg acggtaaaat tgtaatagcg gatgacctat taagtatttt attgtcgtgc
     7021 cgcgatcata ttgcacgcct agtggaaaat gccaaacaca acacccgccc cgatcatgaa
     7081 tgcttggcac aaagtgagcg cttaatgaat gctctggcgc cttgggtgtt agatccacag
     7141 acagcgaaca gcaccaaagc cgcaccccct gttgagacgg ttatagctga tgatattgat
     7201 gctcaactgg aggcggaaaa tagccgcgat ctgtggttta ttgcagttcg ctttggagat
     7261 gatttattgc gcaatggcat ggaccctctc tcgattctgc gatttttaaa tacccttggg
     7321 gaaattgtcg ccatctctat taacaccgac aggttcccgc cacctgatga attcgacccg
     7381 gaaacgttat atctgcaatt tgaattgtat ttgcgtagtt ctgcctcgct agaggaaata
     7441 gaaaatgctt ttgtttttgt gcgagaggaa tcacacatta aaattgtgcc gccgcgcagt
     7501 aatacggcag ctcagactga tttgattaac gccatgccaa agagcaatca gcggctgggc
     7561 gaaattctgg tttcctccca cgcaatttcg ccggaggaat tagagtttgc aattgatacc
     7621 caaaaacaac aagccaaaga gcaggcgcct gtggcattgt tgggtgatat tttaattgat
     7681 acccaggcta ccgcaccgga ggttattcat gccgccttaa gcaggcagca ggcagccccc
     7741 actgaaagcc gtgttgcctc gcaagagcag cgttatattc gcgtggaggc agagcggctg
     7801 gataaactta ttaatttaat tggcgagttg gtgaccaatc ggcagcgggt ggatttattg
     7861 gctgcgggcc ttggcaatac acaactgacg gaggcggtta cttccattgg cagttttacc
     7921 gagcagattc gcggtgctgc gctgacgctg cgtatggtgg caatcggcga tacatttttg
     7981 cgctttaaac gcgtagtgcg cgataccgcc aaagaattag gcaaagaaat tgagttggaa
     8041 attgaaggcg ctgaaacgga attagatcgg gccatggtag aaaaacttaa cgacccttta
     8101 atgcatatag tgcgcaatgc catggatcac ggtatcgaac ctatagcgct tcggcgcgaa
     8161 cgcaaaaaac ccgatgccgg caccattacc ttaaaagcca gccacggcgc aggcaatatt
     8221 ctcatcgaga tctccgacga tggcggcggc ttggatttag agcgcattcg ccgcaaggca
     8281 attgccaata acttgattga tgaaaatgcc cagttaagcc gccaggattt gtgc
//
LOCUS       DAOQGS010000124         8290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4093_length_8290_cov_5.660352, whole genome shotgun sequence.
ACCESSION   DAOQGS010000124 DAOQGS010000000
VERSION     DAOQGS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4093_length_8290_cov_5.660352"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /gene="rsmG"
                     /locus_tag="PKZ52_06385"
     CDS             <1..545
                     /gene="rsmG"
                     /locus_tag="PKZ52_06385"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNG59434.1"
                     /translation="ALFAKWNRAYNLSAIRDPEQMVRLHLLDSLAIVPHISGERLIDV
                     GTGGGLPGIPLAICFPHTAIGLLDSAGKKTRFLFQVKTDLSLVNVQIHNCRVEDHRPV
                     DLYDGVVSRAFASLADMTQSCGHLLKSGGTFWAMKGVYPTAELAALADPIHLRHSITL
                     HVPGLDAERCLLALTASDET"
     gene            549..1346
                     /locus_tag="PKZ52_06390"
     CDS             549..1346
                     /locus_tag="PKZ52_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNG59435.1"
                     /translation="MARIYAITNQKGGVGKTTSCVNLAASLAATKRRVLLVDMDPQGN
                     ATMGSGVDKNTTPKTVYDVLMGHADVASASVYSRDAGFSVLAANGDLTAAEVQMLKLP
                     NKEQRLLKALQLVSNLYDYILIDCPPSLNMLTVNALVACEGVIIPMQCEYYALEGLSA
                     LINTINQVQATINPRLKIEGILRTMYDPRMSLTTDVSAQLTEFFGDKVYRTCVPRNVR
                     LAEAPSHGKPVLAYDKQSKGALAYLALAGEIIRQNEAINEPLLDVAP"
     gene            1369..2289
                     /locus_tag="PKZ52_06395"
     CDS             1369..2289
                     /locus_tag="PKZ52_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNG59436.1"
                     /translation="MKRKSLGRGLDALLGGVNKSPSDAIKAAMNVAAVPVPDAGPMDG
                     KYAELPVEFLQRGKYQPRRDIQNESLQELADSIKAQGVMQPIVVRLIGPERYEIIAGE
                     RRWRASQLAGLDRVPAIIRDVPDETAIAMALIENIQREDLNPVEEAVALKRLQDEFEL
                     THQQVADAVGKSRAAVSNSLRLLNLSNEVRTLLEHGDLEMGHARCLLSLEPGQQSALA
                     RQIIARALSVRQAEALVRKTQQEEAMDKVDPPKNLSPDLKKLEEGLMGRLGVKVQVQH
                     SARGAGKLVLKYKSLDELDGLLARLEYKEQ"
     gene            complement(2578..3438)
                     /locus_tag="PKZ52_06400"
     CDS             complement(2578..3438)
                     /locus_tag="PKZ52_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59437.1"
                     /translation="MLRHSLLLLPLLSISAVSYAGSQLRLFAGIDSTFISTEEIDTRT
                     YRDPTTSKTVTESYHPNEESGSLLGGGVGFEWLSANGVYYGGTLDVSDGDVEYTGESN
                     LGTKIKNGTSYIYYWRPKVYAGKEIREWWFKPRLEIEVGGQLKERHIDPRTEGVEGYK
                     ESYKWWYSGVGVSFEFLGNQDWSWRSGLNYRMMIQPTNDTTLTKRDIDLGSTSSISWL
                     NSVWLNLGEDLYMGIELNLTRMNLERSRLIYDGVVETEESTTPYWYYQPESHWQDTHL
                     RFSISKRFDF"
     gene            complement(3535..4710)
                     /locus_tag="PKZ52_06405"
     CDS             complement(3535..4710)
                     /locus_tag="PKZ52_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59438.1"
                     /translation="MLRYPSTKQILTVAVLSALLAGCGKDSKKATADSNIMDAPPMAE
                     RPQASNAPVDLGSELQLTESSDVARQDQAVALAAIVLTTTLNMDKGSSAVKSAISVFA
                     GAQPKEVATGAMSPVLGGTTQKCAKGGNQTITLLMGEGVGVKQTGAFNKEEVYVSADF
                     DECNLGAAKIDGGLGIGLKLGLAELLNTASFRLETSLIAQNLKINQNGREFYAEGEAG
                     YKFYTNNGYELNTELYADNFELNDGLKMNLLDLHLKQINNTSNTNWSMSISAKYQAGK
                     SNLDSTYVSVSTDDSAPLKGKGYGYPTDGVIRVDTKKPDPSIPLSNSVIIKIAPTAVS
                     NNFPEGTRVQIEIHKADGSIDTALGYSWAALEEVAKNYIKSGVLKLPDGYEDVGEEN"
     gene            5213..5581
                     /locus_tag="PKZ52_06410"
     CDS             5213..5581
                     /locus_tag="PKZ52_06410"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNG59439.1"
                     /translation="MANPVWFCLSRQLCLMLVLLGLVSLVQVEWVVPAALGAALFIVP
                     QLYFTHYTFRYRGLSNAALMVYSSKWGELGKFLLTVSGFALLWRFYPSVNVIVLMSVY
                     GVFWLAQLFIANALMKTLPQ"
     gene            5614..6528
                     /gene="atpB"
                     /locus_tag="PKZ52_06415"
     CDS             5614..6528
                     /gene="atpB"
                     /locus_tag="PKZ52_06415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNG59440.1"
                     /translation="MSSETKTSTEYIQHHLQNLAYGKLPAGYHRHSGEVVAQDTWTLA
                     HSPQEAKDMGFSCIHLDTLGWGIGLAVLLGFLFTRVAKTATVGKPKGLQSFVEMIVEF
                     VNNTVNDVFHHKNRMVAPMALTIFTWVLMMNLMDLIPIDWLPLLATTVTGNEHFYFKV
                     VPTTDPNATLGMSITVFLLMIGFSIKQKGIVGFIKELTMHPFHSGKWFIDIFLIPVNF
                     VLEMVGLVAKPVSLGLRLFGNMYAGELIFILIAIMFSSGVIAALSGGILQWIWAAFHL
                     LVIPIQAFVFMILTTVYMAMAYDVAEEH"
     gene            6573..6821
                     /gene="atpE"
                     /locus_tag="PKZ52_06420"
     CDS             6573..6821
                     /gene="atpE"
                     /locus_tag="PKZ52_06420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNG59441.1"
                     /translation="MTAQALVYIAAALLIGLGALGTGIGFGSLGGKLLEAIGRQPEQG
                     PALMGKMFLMAGLLDAVPMVSVGMGMYMIFSVAPGITG"
     gene            6861..7331
                     /locus_tag="PKZ52_06425"
     CDS             6861..7331
                     /locus_tag="PKZ52_06425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNG59442.1"
                     /translation="MNINLTLIGQSVTFLCFVFFCMKYVWPALIGVMEAREKRIAQGL
                     DNADKADKDLELAKQQAAAQIKEAKEQAAAIVDAANKRANQIVADAKQQAVVEAERVK
                     ASAQAEIEREVTQAREQLRSKVATLALVGAEKVLGAAIDVNAHNQMLDKLAAEL"
     gene            7344..7880
                     /locus_tag="PKZ52_06430"
     CDS             7344..7880
                     /locus_tag="PKZ52_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNG59443.1"
                     /translation="MAELTTTARPYARAAFELARAEGQLDVWAQALSLLAAICSDAKI
                     AGLINSPSLTAAGKSDLLKDLCGEALGQKQQNFVVTLADNKRLSLLPEVNRLFLSFKA
                     EYDKTLDVEVSTAFELSPEWQAKLATALSSKLACQVNLTTQLDKSLLGGALIRAGDTV
                     IDGSVKGRLAKLAAAMHA"
     gene            7905..>8290
                     /locus_tag="PKZ52_06435"
     CDS             7905..>8290
                     /locus_tag="PKZ52_06435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470438.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNG59444.1"
                     /translation="MQQLNPSEISEIIKQRIESISVATEASNQGTVVSVTDGIIRIHG
                     LADVMYGEMIEFASGVFGIALNLERDSVGAVVLGDYRGVAEGQSCKCTGRILEVPVGP
                     ELQGRVVDALGNPIDGKGPLNAKLTDA"
BASE COUNT         1968 a         2033 c         2087 g         2202 t
ORIGIN      
        1 tggccctgtt tgccaaatgg aaccgggcct ataacctgtc cgccatccgc gaccctgagc
       61 agatggtgcg cctgcacctg ttggattccc tcgccatagt gccccacatc agcggcgagc
      121 gcctgattga cgtgggcacc ggcggcggcc taccgggcat acccctggcc atttgttttc
      181 cgcacactgc cataggcctg ctcgattccg ccgggaaaaa aacccgcttt ttatttcagg
      241 tgaaaaccga cctaagtctt gttaatgtgc agatccacaa ctgccgggtg gaagatcata
      301 ggccagtcga tttgtatgac ggcgttgtca gccgagcctt cgcatccctt gccgatatga
      361 cacaaagctg tggccatcta ttaaaatccg gtggcacatt ttgggcgatg aaaggcgtgt
      421 acccaacggc tgagctggcc gccctggccg accctattca tctgcggcac agtatcacct
      481 tgcatgtgcc cggcctggac gccgaacgct gcctgttagc cctaaccgcc tccgacgaga
      541 cttgacctgt ggcccgaatt tacgcgatta ccaatcaaaa aggcggtgtg ggtaaaacca
      601 cctcctgtgt caatttggcg gcctccttag ccgccaccaa gcgtcgcgtt ttattggtgg
      661 atatggaccc ccaaggcaat gccaccatgg gcagcggagt ggataaaaac accacgccca
      721 aaaccgtcta cgacgtgctg atgggccatg ccgatgtcgc cagcgccagt gtttattcgc
      781 gggatgcggg ttttagtgtg ctggccgcca acggcgatct caccgccgcc gaagtgcaaa
      841 tgctcaaatt acccaacaaa gagcagcgcc tgttaaaagc cctgcagttg gtgagcaatc
      901 tgtacgacta catcctcatc gactgcccgc cctcgcttaa tatgctcacc gtaaatgctc
      961 tggtggcctg tgagggggtg attatcccta tgcagtgcga gtactacgcc ttagaggggc
     1021 tatcggcgct gatcaacacc atcaaccagg tgcaagccac catcaacccg cgcctgaaaa
     1081 tcgagggcat actgcgcacc atgtacgacc cgcgtatgag ccttactacc gatgtttcag
     1141 cccagttgac ggaattcttc ggcgataagg tgtaccgcac ctgtgtgccc cgcaacgtgc
     1201 gcctggcgga agctcccagc cacggcaagc ccgtgctcgc ctacgacaaa caatccaaag
     1261 gcgcccttgc ttacctggcc ttggccggtg aaatcattcg gcaaaacgaa gccatcaacg
     1321 agccgctgct cgatgtggcg ccttaagtct ttgtaaggaa attccctcat gaaacgtaaa
     1381 agcctagggc gcggcctcga cgctcttttg ggtggcgtca acaaaagccc cagcgacgcc
     1441 atcaaagccg ccatgaatgt agccgccgta cccgtgcccg acgctggccc catggacggc
     1501 aaatacgctg aattacctgt ggagtttttg cagcgcggta aataccagcc gcgccgggac
     1561 atccaaaacg aatccctgca ggaattagct gactccatca aagcgcaagg cgtgatgcag
     1621 cccatagttg tgcgcctgat cggccccgag cgctatgaaa ttatcgctgg cgagcgccgc
     1681 tggcgcgcct cccaattggc tggccttgac cgtgtacccg ccatcatccg cgacgtgccc
     1741 gatgaaacgg ccatcgccat ggccttaata gagaacatcc agcgcgagga tctcaaccca
     1801 gttgaagaag cggtggcact caagcgctta caagacgaat ttgaactgac ccaccagcag
     1861 gtggccgatg ccgtcggcaa atcccgcgcc gccgtctcca acagcctgcg cctacttaat
     1921 ctgagcaatg aagtgcgcac actcctcgaa cacggcgacc tagaaatggg ccacgcccgc
     1981 tgcctgctca gcctagagcc aggccaacag agcgccctgg cccgccaaat tatcgcccgg
     2041 gccctaagcg tgcgccaggc ggaagcccta gtgcgcaaga cccagcaaga agaagccatg
     2101 gacaaggtag acccgccgaa aaacctgtca ccggatctga aaaaactcga agagggcttg
     2161 atggggcgtt tgggggttaa ggtgcaggta caacactcgg cgcgaggtgc gggtaaattg
     2221 gtgttgaaat acaaaagttt ggatgagtta gatgggttgt tagcgcggct ggagtacaaa
     2281 gagcagtagc agtgagcttt cctgaacatg cacctcgcct gcgcgccgca aattggtttt
     2341 tgctaacgca tttttacatt ggccttattg ttcgaaaaat aacgacgtgc caactatttt
     2401 gttttgaggt gcattaaata atcagatgtt tgccttcgtt gttggtttgt tgggtgcacc
     2461 gctattaatt cgggattatt ttttcagatt tttagcgctt gaggcgtctt tcttcggcag
     2521 gaataaaaat ccgcaggtgt aactgcggat ttttattctg gaaccaatcc tatttattta
     2581 aaaatcgaat cgtttgctta tggaaaagcg cagatgagta tcttgccaat gtgactcagg
     2641 ttggtagtac cagtaggggg tcgtggattc ttccgtttca accacaccgt cataaattaa
     2701 acgactgcgc tctaaattca ttctggtcaa attcagttcg atccccatgt ataggtcttc
     2761 acctaggttt agccagacac tgttaagcca actaatacta gaagtcgatc ctaggtcaat
     2821 atcccgttta gtgagtgtcg tgtcatttgt tggttgtatc atcatgcggt agttcaaacc
     2881 gctgcgccat gaccaatctt gatttcctaa aaactcgaag ctcacgccga caccagagta
     2941 ccaccactta taactttctt tatagccttc aactccttct gttcgaggat caatgtgccg
     3001 ctcttttaac tgcccaccta cctctatctc caaccgtggc ttgaaccacc actctcggat
     3061 ttccttgcct gcatagacct tgggacgcca ataataaatg taggacgtgc cattcttgat
     3121 ctttgtgcct aaattacttt cgcccgtgta ttcaacatcc ccatcgctga cgtcaagagt
     3181 tcctccgtaa taaacaccat ttgccgagag ccactcaaaa cccacaccgc cgccgagcag
     3241 agagcccgac tcctcgttgg gatgataact ttcagtcacc gtttttgagg tggttgggtc
     3301 tcggtatgtc ctggtgtcaa tttcttctgt ggagataaag gttgaatcaa tgccagcaaa
     3361 tagtctcagt tgactgcctg cataactgac ggcgctgatt gataaaagtg gaagtagcag
     3421 caaactgtgc ctgagcatgt aaagttccta aaatactcga tcaagaaata agaaaaagcc
     3481 cgcatgtgcg ggctggaagt ataacgttta ctacaatatt tgaagaagga gttactagtt
     3541 ttcctcgcct acatcttcat agccatcagg caacttcaat acgccggact taatatagtt
     3601 cttcgctact tcctctaaag cggcccaaga gtagcctaag gccgtgtcga tactaccatc
     3661 tgctttatga atttcaattt gaacccgagt tccctcaggg aaattattcg aaactgctgt
     3721 tggcgcaatt ttaataataa cgctattgct cagaggtata cttggatcag gcttcttggt
     3781 atctactctg ataacaccat ctgtggggta tccatatccc ttgcctttga gtggggcgct
     3841 gtcatcagta gaaacggaca cataagtgct atccagatta gacttaccgg cttggtactt
     3901 ggcgcttatg ctcattgacc aattggtatt ggacgtgttg ttgatctgct taaggtgtaa
     3961 atccagcaga ttcattttta aaccatcgtt tagttcaaaa ttgtctgcat aaagctcagt
     4021 attgagttcg tacccattgt tggtgtagaa cttatagcca gcctcgcctt cggcataaaa
     4081 ctcacggccg ttttgattta tttttagatt ctgggcaatc agcgacgtct cgagacggaa
     4141 tgatgcggtg ttcagcagct ccgctagccc aagtttaaga cctatgccca ggccgccatc
     4201 gatcttagct gctccgaggt tgcattcatc gaagtcggca cttacatata cctcttcttt
     4261 gttgaatgca cctgtttgtt tgactccaac gccttcgccc ataagtaggg ttatagtctg
     4321 gttgccacct ttggcgcatt tctgcgtagt cccccccaga actggcgaca tggcgcctgt
     4381 tgcaacctcc ttgggttggg ctccagcgaa tactgaaatt gcgcttttga ctgcgctgct
     4441 gcctttgtcc atgttcaaag tagtagttag tacaattgca gctaacgcaa ccgcttggtc
     4501 ctggcgagca acatctgacg attcggtcag ctgcagctcg ctacccagat ctacgggcgc
     4561 attgctagct tgtggccgtt ctgccatggg aggcgcatcc ataatattgc tgtcggcggt
     4621 agccttttta ctatctttac cgcaaccagc caaaagtgcg ctgagtacag caacggtgag
     4681 gatttgtttg gtagaaggat acctgagcat gcttcttact ccgtgttagt gagagtcttg
     4741 ggtgcttgcg cctagcgcgt tgtagtatgc cacagcttta atagcgtgca gcaagcctcg
     4801 gctttattgt ttccgtttta attttcgggc cattttcgtg ttttctcccg agagcatagt
     4861 tgactacata tgccaggaga ataaaagcga ctcggtcaac ggaattgtgt gtgcttcatc
     4921 atgttgtggt gcggttatcg tacaaaaggg gttttcttga ccaaatatgg gaacttatcg
     4981 ccatattgac cggatgtgcg ttcgcgcagt gtggggcttt ggctggcgaa gattttaatc
     5041 gtcggcgggt attgttggtg tgagcaattt acattttaaa aaggttgatc cagggggggg
     5101 ataaatttat aatcccgcgc cgcttccggt tggcgttgaa gcgtctgaat gttcagaatt
     5161 ttttgaaatc taaaaacggc attttgggct gttttgtgca ggggtgtttt tggtggcaaa
     5221 cccagtttgg ttttgtttgt cccgccagct ctgtctgatg ctggtattgc tgggtttggt
     5281 cagtctggtg caggttgagt gggtagtacc cgccgcgcta ggcgccgcgc tttttattgt
     5341 gccgcaattg tattttacgc actacacatt ccgctatcgc ggtttgagta atgctgccct
     5401 gatggtttat tcctcgaaat ggggtgaatt gggcaagttt ctgttaactg tatcgggatt
     5461 tgcactgctt tggcgcttct atcccagcgt caatgtaata gtgctgatga gtgtttatgg
     5521 tgtgttttgg ttggctcaat tgtttatagc taacgccttg atgaaaacgc tcccgcagta
     5581 accaaattct aaattttgtc gaagatccgc actatgtcca gcgaaacaaa aacctctaca
     5641 gaatatattc agcatcacct tcaaaatctc gcctacggca aattgcccgc tggttatcac
     5701 cgtcacagcg gtgaggttgt ggctcaggat acttggaccc tcgcgcacag cccgcaggaa
     5761 gccaaagata tgggttttag ctgcatccac ttggataccc tcggctgggg aattggcctt
     5821 gcggttttgt tgggctttct atttactcgc gtcgccaaaa ccgccactgt tggcaaaccc
     5881 aagggtttgc aaagttttgt cgaaatgatt gttgagttcg tcaataacac cgtaaacgac
     5941 gtgtttcatc ataaaaatcg tatggttgca cccatggcat tgacgatttt tacttgggtg
     6001 ttgatgatga accttatgga tttaattcct attgattggc ttccgttgct cgctactaca
     6061 gtaactggca atgaacactt ctactttaag gttgtgccaa ctaccgatcc taatgcaact
     6121 ttgggcatgt caataactgt gtttttgttg atgatcggtt ttagtatcaa acagaaaggt
     6181 atcgtaggat ttattaaaga attaaccatg cacccatttc attctggcaa atggtttatc
     6241 gatatctttt taataccagt taactttgta ctggaaatgg tgggcctagt ggcgaaaccc
     6301 gtgtccttgg gtttgcggtt gttcggcaac atgtacgcag gcgaattaat ttttattctt
     6361 atcgccataa tgttcagctc cggagtaatc gctgctcttt ctggcggtat tctgcaatgg
     6421 atatgggcag cattccactt gctggtaatt cctattcaag ccttcgtatt tatgattttg
     6481 accacggttt atatggctat ggcctatgac gtggccgaag aacactaatt ttatttttac
     6541 tttttatttt taactttaag aggaaacaag aaatgacggc acaagcactg gtttatatcg
     6601 cagcggctct tctgatcggt ttgggcgctt tgggtacagg tatcggcttc ggatctttag
     6661 gcggaaaatt acttgaagca atcggccgtc aacctgagca aggcccggct ctgatgggaa
     6721 aaatgttctt aatggcaggt ttattggatg cggtacccat ggtgagcgtt ggtatgggta
     6781 tgtacatgat tttctcggtt gccccaggaa ttaccggtta attccattag cccccattaa
     6841 ttgcgaatag aggtgttggt gtgaatatca acctaacgct aatcggacaa tcagtaactt
     6901 tcttgtgctt cgtatttttc tgtatgaagt atgtgtggcc tgcgctcatt ggtgtgatgg
     6961 aagctcggga aaaacgcatt gctcaagggt tggataacgc cgataaagcc gataaagatt
     7021 tggagttggc caagcaacaa gccgccgcac aaattaaaga agcaaaagaa caggccgccg
     7081 ctattgtgga tgccgctaac aaacgcgcca atcaaatcgt ggctgatgct aaacagcagg
     7141 ccgtggtcga ggctgagcgc gttaaggcat ctgcacaggc tgaaatcgag cgcgaagtaa
     7201 cgcaggcgcg ggaacagttg cgcagcaaag tagcaacgct tgccttagtg ggtgctgaaa
     7261 aggtactcgg ggcggctatt gatgtgaatg cgcataatca aatgctcgat aaactcgcag
     7321 ctgaactcta atagggggag accatggcgg aattaaccac cacagctcgc ccctacgcgc
     7381 gggcggcttt tgagcttgcg cgcgcggaag gtcagttgga tgtttgggcg caggcgctca
     7441 gtttgctggc tgctatttgt agcgacgcaa aaattgccgg tttaattaat tcgcccagtc
     7501 ttactgctgc gggtaaaagt gacctgctaa aagatttatg tggtgaagct ctggggcaaa
     7561 agcaacaaaa ttttgttgtt accttggcgg acaataagcg tttgagtctt cttccagagg
     7621 tcaatcgctt gttcttatcc ttcaaagcgg aatacgacaa aaccctggat gtggaagtga
     7681 gcaccgcgtt tgaattgagc ccagagtggc aggctaaatt agccaccgcg cttagctcta
     7741 aactcgcttg ccaagtgaat ctcaccacac agttggataa aagtttgtta ggcggcgcat
     7801 taattcgcgc cggagacacc gtcattgatg gttcggtgaa aggccgtttg gctaaactcg
     7861 ccgcagccat gcacgcctag tcattaggtt tgaggaattt gagtatgcaa caactgaatc
     7921 cgtcagaaat tagtgaaatc attaagcagc gcattgaaag cattagcgtt gccactgaag
     7981 ccagtaacca aggtaccgtc gtttccgtaa ccgacggtat tattcgtatc cacggccttg
     8041 ccgatgtgat gtacggggaa atgatcgagt tcgccagtgg cgtattcggt atagctctga
     8101 acttagagcg cgactccgta ggtgcagtgg tattaggtga ttaccgcggc gtcgccgaag
     8161 ggcagagctg taaatgtacc ggccgtattt tggaagtacc tgtaggccct gaattacaag
     8221 gccgtgtggt agacgcactg ggaaatccca tcgatggcaa aggcccgtta aatgccaaat
     8281 taaccgacgc
//
LOCUS       DAOQGS010000125         8266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4104_length_8266_cov_4.991718, whole genome shotgun sequence.
ACCESSION   DAOQGS010000125 DAOQGS010000000
VERSION     DAOQGS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_8266_cov_4.991718"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /gene="tssC"
                     /locus_tag="PKZ52_06440"
     CDS             complement(<1..1345)
                     /gene="tssC"
                     /locus_tag="PKZ52_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNG59445.1"
                     /translation="MSSASPAFNFDEILKQQASAVRLDDFLREPHWPNALRYWLKHHT
                     PEGAVNNTRDIISQLELAISHLDQLINQQLNAIIHHPKFQRLESSWRGLYYLTLQADG
                     IKLAKIKVLDASWQEVTKDLSRALEFDQSSLYNKIYAEEYGTPGGEPYGALIGDYAIS
                     HKQTRTHPYDDISALDSIAQISAAAFAPFIAGVSSELLGMDDFSTLRLPLNLTSLFAQ
                     EEYIQWRALRMKNEARFVGLTLPRILMRKPYRTTPGNYRGLYFCEYIRSPEDYLWGNA
                     AYAFGGILLREFGSVGWFGHIRGVPRNNIGGGLVTNLPCDSFKTDPNDIITKPATDVV
                     ITDNIERDISNLGFIPLCQCFATPYFAFYSNQSIQLSKRMATRDAQVNAKLSTMLQHV
                     LCASRVAHYIKVMVRDKIGSFITADQCEDFLRRWLHQYTTGRKDMEWEEQALLLQQ"
     gene            complement(1467..2957)
                     /gene="tssC"
                     /locus_tag="PKZ52_06445"
     CDS             complement(1467..2957)
                     /gene="tssC"
                     /locus_tag="PKZ52_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNG59446.1"
                     /translation="MADTELESQGAGSAEAQELSYLSMALDATRQTSRDDAEDLLKNL
                     TREVMAGTVKWNKNLTVTINSAIAAIDKALSKQLTAIMQHPKFTKLEGSWRGLNYLVS
                     NSETSSQLKIRVLNASKKEISKDLERAVEFDQSNLFKKIYESEFGTAGGEPYSCLIGD
                     YEFSAHPDDVSTLTNISGVAAAGFCPFISAAGPSMFGFNSYQELSKPRDLEKIFESSE
                     YISWRSFRESDDSRFVSLVMPRVLARLPYGASTKAIDAFNFEEADTNDQGKHLGIEHD
                     NYCWMNAAYAMGTTLTRAFAEYGWCTAIRGAEGGGKVEGLPSHIFASDDGGIDQKCPT
                     EIGITDRREAELSKLGFLPLLHYKNTDYAVFFGAQTTQKPAQFQSPDATANAAISARL
                     PYIMATSRIAHFLKVMARDKIGSFMEADEAERMLNQWIKQYVNGNPLASAESKAKYPL
                     AEARVEVKEVPGQPGVYNAVAWMRPWLQMEELTTSLRLVAKMPKAG"
     gene            complement(2957..3460)
                     /gene="tssB"
                     /locus_tag="PKZ52_06450"
     CDS             complement(2957..3460)
                     /gene="tssB"
                     /locus_tag="PKZ52_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNG59447.1"
                     /translation="MASIHDKLNRVRKPRVHITYDLESNGESVKRELPFVMGVMGDYS
                     GDNTNQKAFKDRNFLQVDRDNINEIMNNINPQLNMKVENTLQGDGTEMSVNLSLNHLE
                     DFEPHRVVEQVEPLRKLLEARNKLRDLLSKADRSEELEKVLEDILSNTDALNSLKGEL
                     GINEETK"
     gene            complement(3971..5116)
                     /gene="tssA"
                     /locus_tag="PKZ52_06455"
     CDS             complement(3971..5116)
                     /gene="tssA"
                     /locus_tag="PKZ52_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HNG59448.1"
                     /translation="MSSRSIDIAAFLSPISEEAPCGRDLREGGSPTSNFQMVKLARST
                     ARSAERSNVYSDDGQAADDARRKIDESWRKIIELAPKILKSESKDLEVACWYLEALVR
                     RDGIAGLRDGFELIEGLIENFWPDLFPMPDEYGLETRVGSLKGLNGEGGSDGALMAPI
                     RNSIITNSGQHRAYSFWEYQSAAETSRILDDKSREKEYARKGYTLQNIETAVRESSSD
                     FLTDLRNDLVGALDSFTRFNDKLYQLCGASEAPPSSAITNLLKDCITAVTFLGGNKIV
                     AETSTVDDSLNAAQESDHSDNAAAGAGAAAVQPVTRALANRDQAFKQLAEIAEFFRKT
                     EPHSPISYMLEKAVRWGNMPLHELMQELISDDATRNRYSELTGVKGQ"
     gene            5491..7434
                     /gene="tagH"
                     /locus_tag="PKZ52_06460"
     CDS             5491..7434
                     /gene="tagH"
                     /locus_tag="PKZ52_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HNG59449.1"
                     /translation="MKITFSLLKAPAKAALQQGQKSFDEQGGSIGRGDGNTWVLPDPD
                     KFLSSKHCVVSFEQGQFYITDTSTNGLFLNGASLPVGRNNRVGVSEGDTLDLGEYRLR
                     VGLEKTAAKISPFADIAPAEPPPAFNAGAQWGALEPTPQAIDGADLFPVGGGAGGDPF
                     YDASMDPFAAKDPFGLGGAKPADPFANPADPFAKIAGNPQDNPFAADPFGGGDMFGGP
                     DPFSPAKPQAVPGFTQQEGHNPLHDAISLPKIAANPPGSAAIPDDFWNSLGGAAAADE
                     LGMPAFKPVDPFASGDPLAAAPASPAARPFAPPPVAPVPPPPPAFRPEPASPPPVAPV
                     PDALGDNPFAHLGQSAGATPVPLDASEGQVYPIGSAFSTHVPASTPGKVPLSDRLKAM
                     QGGEANDTSAGWATPPEPQVPPQAPPVAPPVPPIAAQPVQPIAPPAARQAPIHDTGVQ
                     NLIAALGLGGQLSAEQEQVLPQLIGEMTRESISGLLQILRARNSIKNEFRIAVTTIQP
                     RENNPIKFATDVDDALELLFVRSSKGYKPPVESVQDSFESIMDHQVAILAGIRAAFKS
                     LLSRFDPLQLEQQFERQGKTGGILPGMKGAKCWSAYDDFYQDLIGNFENSFQNLFGDE
                     FVQAYEEQLRKLAIARKREHSQR"
     gene            7443..7958
                     /gene="tssJ"
                     /locus_tag="PKZ52_06465"
     CDS             7443..7958
                     /gene="tssJ"
                     /locus_tag="PKZ52_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HNG59450.1"
                     /translation="MHIRTPSIWNTLWQLTLLLGLGLGLASCTKVNGTGKTIGLDTDL
                     KLTIQADMDINPDANNRPSPVFVRMYELKSPMIFHQADFMTLYNKDKAVLEADFVNRQ
                     DLKIFVPGDKRESNLVLNPGTKFVALYAEFLQYQNANYKVIFPVTPGNIVGNRISVKI
                     TGNHMVLVEKK"
     gene            8021..>8266
                     /gene="tssK"
                     /locus_tag="PKZ52_06470"
     CDS             8021..>8266
                     /gene="tssK"
                     /locus_tag="PKZ52_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNG59451.1"
                     /translation="MSLESKVIWSEGMFLNPQHFQQQERYIERYVDGRISTAGAYLWG
                     FFDCEIDQQLLKLGKISLSHERGVFPDGTPFNFPDIDE"
BASE COUNT         1802 a         2095 c         2305 g         2064 t
ORIGIN      
        1 gttgttggag aaggagggct tgttcttccc attccatatc cttgcggccg gtggtgtatt
       61 gatgcagcca gcggcgtaaa aaatcttcgc actgatcggc ggtgatgaag ctgccgattt
      121 tatcccgcac catcacttta atgtagtgag ctacgcggga ggcgcataaa acgtgttgca
      181 gcatggtgga taatttggcg ttgacctggg catcccgcgt agccatgcgt ttggataatt
      241 gaatagactg gttggaataa aaagcaaaat agggtgtggc aaaacattgg cagaggggaa
      301 taaaacctaa attgctaatg tcccgctcga tattatcggt aatcacaaca tcggtggcgg
      361 gtttggtgat gatgtcgttt gggtcggttt taaaactgtc gcagggtaaa ttggtaacta
      421 aaccgccgcc gatattattg cgcggcacac cgcgtatatg gccaaaccag cccacgctgc
      481 caaattcccg cagcaaaata ccgccgaagg cataggcggc attgccccat aaataatcct
      541 ctggcgagcg gatatattca caaaaatata aaccgcgata attgcccggc gtagtgcggt
      601 agggtttgcg cattaaaata cgcggcaggg taaggcctac aaaacgcgcc tcgtttttca
      661 tgcgcagggc gcgccactgg atatattcct cctgagcaaa tagcgaggtt aaattcagcg
      721 gcaaacgcag ggtggaaaaa tcatccatgc ctaataattc gctggatact cccgcaataa
      781 aaggtgcaaa agccgctgcg gaaatttgtg cgatggaatc tagggcagaa atatcatcgt
      841 agggatgggt gcgggtctgt ttatggctga tggcgtaatc gccaattaat gcgccgtagg
      901 gttcaccgcc gggggtgccg tattcttcgg cgtagatttt gttgtacagg ctgctttggt
      961 caaattccaa cgcgcggctt aagtctttag tgacttcctg ccagctggcg tcgagcactt
     1021 taattttggc taatttaata ccgtccgcct gtagggttaa ataatacagg ccgcgccagg
     1081 aggattctaa acgctgaaat ttcgggtggt gaattatggc gttgagctgc tggttaatta
     1141 actggtcgag gtggctaatg gccagttcaa gttggctaat aatatcccga gtattgttaa
     1201 cagcgccctc gggggtgtga tgttttagcc aataacgcag ggcgttgggc caatggggct
     1261 cgcgcaggaa atcgtctaag cgcacggcgg atgcctgctg ttttaagatt tcgtcaaaat
     1321 taaatgctgg gctggccgat gacactgggt gaattccaac tgaagttcag gaaaaagggc
     1381 ttatatcgat tgagccgaat tttatagggt ttaaaaacac aagggagccg aagctccctt
     1441 gtattaggca cacatcagcg ggtgaattaa cctgctttgg gcattttagc taccaagcgc
     1501 aaggaggttg ttaactcctc catttgcagc caggggcgca tccaggccac agcgttgtac
     1561 acgccaggtt ggccgggtac ttcttttact tccacgcggg cttcggccaa tggatatttg
     1621 gctttggatt cggccgaggc cagtgggttg ccgttgacgt attgcttgat ccactggttc
     1681 agcatgcgct cggcttcatc agcttccata aaggagccga ttttgtcgcg ggccattact
     1741 tttaagaagt gggcgatacg gctggtcgcc atgatgtagg gcaggcgcgc agaaattgcg
     1801 gcgttggccg tggcatcggg ggattggaac tgggcgggtt tttgcgtggt ttgcgccccg
     1861 aaaaataccg cgtagtcggt atttttgtag tgcaacaagg gcaaaaagcc gagtttggag
     1921 agttccgctt cgcggcggtc ggtaataccg atttcggtgg ggcatttttg gtcgatgccg
     1981 ccatcgtcgc tggcgaaaat gtggctgggc aggccttcaa ccttaccgcc gccttcagcg
     2041 ccgcgaatgg ccgtacacca gccgtattcg gcaaaggcgc gggtcagggt ggtgcccatg
     2101 gcgtaggctg cattcatcca gcagtagttg tcgtgttcaa tgcccaggtg tttgccctgg
     2161 tcgttggtgt cggcctcttc aaagttaaac gcgtcgatgg ctttggtgga agcgccgtag
     2221 ggcaggcgcg ccagaacgcg gggcattacc aggctgacaa aacgggagtc gtcgctctcg
     2281 cggaaactgc gccaggaaat atattcggaa gattcaaaga ttttttctaa atcccgcggc
     2341 ttggagagtt cttgatagct gttaaagcca aacatgctcg ggccggcagc agaaataaac
     2401 gggcaaaagc ccgctgcggc aacgcctgag atattggtga gggttgagac atcgtccggg
     2461 tgagcggaga attcatagtc accgatcaag cagctgtagg gctcaccacc tgcggtgcca
     2521 aattcgcttt cgtagatttt cttaaacagg ttgctctggt caaattccac cgcgcgctca
     2581 agatccttgc tgatctcttt tttgctggcg tttaagacgc gaatttttaa ttgtgagctg
     2641 gtttcagagt tggataccag gtagttaagg ccgcgccagg agccctcaag tttggtgaac
     2701 ttggggtgtt gcatgatcgc ggtgagctgt tttgacaggg ctttgtcgat ggcggcgatg
     2761 gcggaattaa tggtaaccgt gaggttttta ttccacttaa cggtgccggc catcacttcg
     2821 cgggtgaggt tttttagcag gtcttcggca tcgtcacggc tggtttgacg ggtggcgtcc
     2881 agggccatgc tcaggtagct aagctcctgg gcttctgcgc tgccggcgcc ttgcgattct
     2941 aattctgtat cggccattat ttggtctcct cattgatgcc caattcgcct ttaaggctat
     3001 tgagtgcgtc tgtgttgctt aaaatgtctt caagaacttt ttccagctct tctgagcggt
     3061 cggccttgct caataagtcg cgcagtttgt tgcgggcttc taagagtttg cgcaggggtt
     3121 caacctgctc caccacgcgg tgaggttcaa agtcttctaa atgatttagg cttaaattca
     3181 ccgacatctc agtgccgtcg ccctgcagag tgttttcaac tttcatgttc aactgggggt
     3241 taatgttgtt catgatttcg ttgatgttat cgcgatcgac ttggaggaaa ttgcgatcct
     3301 taaaggcttt ttggttggtg ttgtcaccgg agtaatcacc cataacgccc attacaaatg
     3361 gcaattcgcg cttgacgctc tcgccgttgg actctaaatc gtaggtgatg tggacgcgcg
     3421 gcttgcgcac gcgattgagt ttgtcatgga tgctggccat agtcctaacc tcgtgatgaa
     3481 aattctagtg ttatgaaatt aaaacctgca catgggcgaa ggcctgcgca gatcccgtgt
     3541 ttttgcccgc tgggagcctt atctcgtgcc ctttatctcg atgacgcctg caatctttat
     3601 gacgccttta tctcaatgac gaaggtgagc agcaatagta cgttaaaagt gcagctgccg
     3661 ctaactgcgc ctacgccact atagctgccg cgccttagac agggcgactt aggtttaatg
     3721 gctgggcggt catgccatcg cggccctatt ttttatcttg ttatatcttt tttatttcga
     3781 aatatctttt taatcgtgca ggttttttat tctgcatgcc agatgcccca tctgcggcac
     3841 ggctcttaac gcgcgtggtg caactgactt ctcttaacgg gtagttctcg tacttacagc
     3901 ctgatttagg tgtgttggtg ttatttactg tgcaggttta agctgcagca atgcgcctac
     3961 tggccttaaa ttattgcccc tttacaccgg ttaactcgct gtagcgattg cgggtggcgt
     4021 catcgctgat taattcttgc atgagttcgt gcaacggcat gttaccccag cgcacggctt
     4081 tttccagcat gtaggaaatg ggcgagtggg gctcagtttt gcggaaaaat tcggcgattt
     4141 ctgctagctg tttaaaggct tggtcgcggt tggcaagggc ccgcgtgacg ggctgcacgg
     4201 ctgcagcacc cgcgccggcc gcggcgttgt cgctgtggtc ggattcctgg gcggcgttga
     4261 ggctgtcatc cacggttgat gtctccgcta cgattttgtt accgccgaga aaggtgacgg
     4321 cggtaataca atctttcagt aaattggtaa tagcgctgga ggggggcgcc tcgctggcac
     4381 cgcataattg atagagcttg tcgttaaagc gcgtaaaact atcgagggcg ccgactaggt
     4441 cgttgcgcaa atcggtgaga aagtcactgc tggattcccg cactgcagtt tcaatatttt
     4501 gcagggtgta gcccttgcgg gcgtattctt tttcgcggga tttatcatcg agaatacggg
     4561 aggtttccgc agcggactgg tactcccaaa aactataggc gcggtgctgg ccgctattgg
     4621 taatgatgct gttgcggatg ggggccatga gggcgccgtc cgagccgcct tcgccattga
     4681 ggccctttaa agaccccacc cgagtttcca agccgtattc atcgggcatg gggaagaggt
     4741 cgggccagaa gttctcgatc agtccttcaa ttaattcaaa gccatcgcga agccctgcaa
     4801 tgccatctcg gcgcaccagg gcttctaaat accagcaggc gacttcaagg tctttggatt
     4861 cggattttaa gattttgggc gcaagctcaa taattttgcg ccaggattcg tcgattttgc
     4921 ggcgggcgtc gtcggcggct tggccgtcat cgctgtagac attgctgcgc tcggcactgc
     4981 gggccgtgga tcgggcgagc ttaaccattt ggaagttgga ggtgggcgag ccgccctcgc
     5041 gcagatcgcg gccgcagggc gcctcctcac tgatcgggct taaaaatgcg gctatatcaa
     5101 ttgagcggga cgacataaat tcctaaatcc tgaccaaggc caaaaaacgc acaagcgaat
     5161 gggggcaagg ccctattcaa atcggtgtcc gcaaccaccc aacacacttc ctggcgcaac
     5221 aatcccgccc aatgcggggc ggtgatttaa accatattgc aggccaactt gcaagccata
     5281 ggcgttaatt aatcctcttt atcggcgcgc cattgaggat cgagttaagt gtgaatttct
     5341 aaatgcgggc taattcctaa gcatggcttt taacccgtct catacccggc atatactgcc
     5401 cgcgcaaata aatacagccg ctgccgcctg gccgacccag ccaggcgcac cagcggcgag
     5461 tacctgggcg gtagcggtgg accttaagtt atgaaaatta ccttttctct gttaaaggcg
     5521 cctgctaagg ccgctttgca gcaggggcaa aaatcctttg acgagcaggg cggcagcatc
     5581 gggcgcggcg acggcaatac ctgggtactg cccgatcccg ataagttttt gtcctccaaa
     5641 cactgtgtgg tcagctttga gcaggggcag ttttacatta ccgataccag caccaacggc
     5701 ctgtttttga acggcgctag cctgccggtg gggcgcaaca accgcgttgg cgtcagcgaa
     5761 ggggatacct tggatttggg cgagtatcgc ctgcgtgtcg gcctggaaaa aaccgcggcg
     5821 aaaatatccc ccttcgccga catagcaccg gcagagccgc cgccagcatt taatgcgggc
     5881 gctcaatggg gcgcacttga gcccactccc caggccatcg atggtgccga cttgttcccc
     5941 gtcgggggcg gtgctggggg cgacccgttt tacgatgcca gcatggatcc tttcgccgct
     6001 aaagacccgt ttggcttagg cggcgctaaa cctgcggacc ccttcgcgaa tcccgccgac
     6061 ccttttgcaa aaattgccgg taacccccaa gacaacccgt ttgctgccga cccgtttggc
     6121 ggcggtgata tgttcggcgg gccggatccg ttttcccccg ccaagcccca ggcggtgccc
     6181 ggttttaccc agcaggaggg ccataaccct ctgcacgatg ccatttcgct tcctaaaatt
     6241 gccgccaacc cgcccggctc ggcggctata cccgatgatt tttggaattc cctcggcggc
     6301 gctgcggcgg cggatgaatt aggcatgcca gcgtttaagc ccgtcgatcc ctttgccagt
     6361 ggcgatcctt tagctgcagc accggcttcg cccgccgccc gcccgtttgc accgccaccg
     6421 gttgcccctg tgccgccacc gccacctgca tttaggccgg agcctgcgag tccgccgcct
     6481 gtggcgcccg tgccagatgc gttgggcgat aatccctttg cccatttggg gcaaagcgcc
     6541 ggggccactc ccgtgcccct ggacgccagt gaagggcagg tgtaccccat tggcagtgcc
     6601 ttctccaccc acgtgcccgc cagtaccccg ggcaaggtgc cgctgtcgga tcggctcaag
     6661 gcgatgcagg gcggtgaggc caatgacacc tccgccggtt gggcaacgcc ccccgaaccg
     6721 caggtgccgc cccaggcccc gccggttgca ccgccagtcc cacccattgc ggcgcaacct
     6781 gtgcagccta ttgcaccgcc cgctgctcgg caggcgccaa tccacgatac gggtgtgcaa
     6841 aacctcattg ccgccttggg cttgggcggc cagttgagcg ctgaacaaga acaggtattg
     6901 ccgcagttaa ttggcgagat gacccgcgaa tccatcagcg gtttgttgca aatcttgcgg
     6961 gcgcgcaatt ccatcaaaaa tgaatttcgc attgccgtta ccaccattca acccagggaa
     7021 aacaatccga tcaaattcgc cacggatgta gacgacgcct tggaattatt atttgtgcgc
     7081 tcctccaagg gctataagcc cccggtggaa tcggtgcagg atagttttga atccatcatg
     7141 gatcatcaag tggcaatttt ggcaggtatt cgcgcggcgt ttaaatccct attatcgcgc
     7201 tttgacccgc tgcagttgga gcaacagttt gagcgccaag gtaaaaccgg cggcatattg
     7261 cccggtatga aaggcgctaa atgttggtcc gcctatgatg atttttatca agatttaatc
     7321 ggcaattttg aaaattcgtt tcaaaactta ttcggcgacg aatttgtgca ggcctatgaa
     7381 gaacagctgc gcaaattggc catcgcccgc aaacgtgaac actctcaacg ataggagccc
     7441 ctatgcacat ccgcacgccg tctatttgga acaccctttg gcaattgacc ctgctgttgg
     7501 ggttaggctt gggtttggcc agttgtacca aagttaatgg cacaggtaaa accatcggcc
     7561 tggatacgga tttaaaactc accattcagg cggatatgga tattaacccg gatgccaata
     7621 accggccttc gccggtgttt gtgcgcatgt acgaattaaa atcgccgatg attttccatc
     7681 aggcagattt tatgacgctg tataacaagg ataaggcggt gttggaggca gatttcgtca
     7741 atcgacaaga cctgaaaata tttgtaccgg gcgataaacg cgagtccaat ttggtgttaa
     7801 accccggcac taaatttgtg gccttatatg cagagttttt acagtatcaa aatgccaatt
     7861 acaaagtgat attcccggta accccgggca acatagtggg caaccgtatt tcagtgaaaa
     7921 ttaccggcaa ccatatggtg ctggtcgaga aaaaatagcc aacctgggtg cacgccaccg
     7981 ggttaatcct ccgctctatg tttaagttca cggatcgttt atgtcgttag aaagcaaagt
     8041 tatttggtct gaggggatgt ttttaaatcc ccaacacttc cagcagcaag agcgttatat
     8101 cgagcgctat gtggatggcc gtatcagtac agcgggtgca tatttatggg gattttttga
     8161 ttgcgaaata gaccagcaat tattaaaact aggcaaaatt tccctaagcc acgagcgtgg
     8221 agtatttccc gatggcacgc cgtttaattt tcccgatatt gacgag
//
LOCUS       DAOQGS010000126         8249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4118_length_8249_cov_4.898096, whole genome shotgun sequence.
ACCESSION   DAOQGS010000126 DAOQGS010000000
VERSION     DAOQGS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_8249_cov_4.898096"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..500)
                     /gene="accB"
                     /locus_tag="PKZ52_06475"
     CDS             complement(27..500)
                     /gene="accB"
                     /locus_tag="PKZ52_06475"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNG59452.1"
                     /translation="MDIRKIKKLIELLEESNVEELEIREGEESVRISRGQRAPAAPVH
                     TYIHQPMPTAPVHQAAAPAAPVSETVAAKKDEPVSSGHAVKSPMVGTFYRASSPGSQP
                     FVAVGQKVKAGDVICIVEAMKMMNQIEADRAGVVEAVLLEDGQPVEFDQPLVIIA"
     gene            complement(526..969)
                     /gene="aroQ"
                     /locus_tag="PKZ52_06480"
     CDS             complement(526..969)
                     /gene="aroQ"
                     /locus_tag="PKZ52_06480"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNG59453.1"
                     /translation="MAKILVLHGPNLNLLGSREPEIYGFTTLSDINNLLASACASHQH
                     EFFHLQSNAEFALIDRIHQAKAEGVDFIIINPAAFTHTSVALRDALAGVHIPFIEVHL
                     SNVHAREPFRHHSYLSDIAKGIICGFGAQSYLLALEAAFSHLKSH"
     gene            complement(1231..3039)
                     /locus_tag="PKZ52_06485"
     CDS             complement(1231..3039)
                     /locus_tag="PKZ52_06485"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNG59454.1"
                     /translation="MKLINRTVFFVLWAFCLSITSLQSLAANDVSSLTGKSATSEEFL
                     PVEQAYKVNVIQEGNAIYLDWTIAKGYYLYKEKFKIFAQTEQSKTPFTPTFEASQSKY
                     DPYFKKDVEIFHDATRITLSTANLPKQFEMKLTSQGCADAGLCYAPHTRYFQIDLSQG
                     TALETAETKIVAAEPAAAATTAQASTTAAEAAPAEETYSFGVYILLAIGGGMILNLMP
                     CVFPVLSLKALTFASTSQDGHSHSSHGWAYTFGVCLSFVIVAIAILALRQAGQGVGWG
                     FQLQNPVFVAFVTYLFLVMGLSLSGMVHFGTSLMGVGQNLTAAEGLRGSFFTGVLAAV
                     VASPCTGPLMAPAIGFALTQPWYVSIIIFIALGFGLALPFLALSYSPALAKMLPRPGM
                     WMETLKQFLAFPMYLTSVWLLWVLGNQVGIDAMAALVLGGIAITFALWLLQNQPEGTV
                     GKTLVKGSAIAALFYAGYVGYNAESFKQKSEGWEAWSAELVEKHRSEGRPVFVDFTAD
                     WCITCKANEANAINREDVSNTFKKLGYATLKGDWTNADPKITAVLSEFKRSGVPLYVV
                     YPAGSGKAELLPQVLTKEIVIEALERNAAGKKVAAN"
     gene            3354..4043
                     /locus_tag="PKZ52_06490"
     CDS             3354..4043
                     /locus_tag="PKZ52_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59455.1"
                     /translation="MSRLAPLFVLQVLLSLAGCVSAPAQPAPPPPVQAKPLDAKAQKI
                     ELLLGRADTALSMQALTEPASENAYDLYRAVVIMDPTNVRGQEGIKKVAEAYPAQVEE
                     AIVGKQYDAALRKLQKGLILFPNNPQLKQMEAQAKAAKLAQTQAASKSTTISLPAADL
                     SAKSPAVETTLRNLAKRVAVSNELLIINARSDAEGRWIYKTMKDAAGARLRGDIKISS
                     TPSIQLHPAAL"
     gene            4537..7035
                     /locus_tag="PKZ52_06495"
     CDS             4537..7035
                     /locus_tag="PKZ52_06495"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNG59456.1"
                     /translation="MTKLLQRIALAQPVKHVVWLLVGLVIIHQVLPITHWQQMLGLWW
                     RLPFNVIVLMVIAAVAAYGLLNAWDFLALKKEAKNHAQKPTSGALLQRMITAQICRAL
                     GVGVQPVVAQAGFLPHAGLLVAGLLACIGAVVVASPELFDLPFKAHVWLAAGIFLLGL
                     CALFVRTLRLGLGRGEGAMVSELIKLVVGREYQNWLKPLTLFLPAALALYIPLNKIVQ
                     MSALHFVGIYLLAHMAGVLSRVPGGAGVFDGIFLIFAAKFYPATQVFICLVAYRAVYY
                     CLPLISGLTAYLLLDIRKNQAMSVKRPWALAQLPGQLFQPLVSLWLLALTVTYWLGHV
                     PPFLNGIIPSPPSFVAPYINNLLSVIFLIIWRCHWRRIDLAYGLVFGCFIVAAALFAL
                     NDALLSSVVCLLMVWIFSSSGHYFYRTSALLQGLIPWALWGRYCLGGFLLVSATVLLT
                     ETNDIERMFISQWISSEVLSPLEKLVFVTTFFAGGLAVYGLFTRCDARLQLPDNAALQ
                     RYRELVQREGDTQCHLGLAGDKYFLFSATGKSCIAFGKTPNQWIAMGDPIGDSTEFAA
                     LLWSLAEQADAAGASLVLYKVDAANLSWYVDLGMSAFKLGDEARVKLAEFSLAGSKKL
                     NLRNNYNKCVKEGLEFAVLEGDSLKAAMPQLAVISQDWLDAKSAREKQFSLGFFSVDY
                     LQFGSVAVAKYQGEIVAFASLWRDITQQEVAIDLMRYGRSAPKRVMEYLTLSTLLWAQ
                     EQGYAWFNLGMAPLSGLQSHRLANVLHKFGGYLFASHTRFYNFSGVYTYKEKFTPQWH
                     GRYLIAKNPWQLSIALVNVAKLIAGGAKGLIAKT"
     gene            7246..>8249
                     /locus_tag="PKZ52_06500"
     CDS             7246..>8249
                     /locus_tag="PKZ52_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNG59457.1"
                     /translation="MKKVKLGVGGLLLVLVVAAGFFAVQYLAPGFNESIHEHHSQVAG
                     LGDVAFFNVDEPDKGHLIVFAASAEPVTERRALARRWADKGYLVSVIDPGFLKGQSAC
                     ADLASPIAQLQHFIEQRYPQLAGLLPLVMGRGEAAALSFIALAQAPAKSFHSAIAVDF
                     CPARALAQTFCPQRAWSGQPGVMAAPSQALGANAYVFQTPRLAASGTCDLVASEQYLQ
                     AVPSVKLSQAQGGADDWLEVDALLNWLDPRLTSQALSAQNDDDSPVVEVPAQSTQSAD
                     YFVLFLTGDGGWAQLDKELAQHFSAQGINVLGFDSLSYFWRKRSPEETAADISALISS
                     "
BASE COUNT         1760 a         1935 c         2401 g         2153 t
ORIGIN      
        1 attttttcga acatcgtagc gtcctgttag gcgatgatga ctaggggctg gtcgaattcg
       61 acgggctgac catcttccaa cagcaccgcc tccacaacac ctgcgcgatc ggcttcaatc
      121 tggttcatca ttttcatggc ctccacgatg cagatgacat cgccggcttt aaccttttgg
      181 cccacggcaa caaagggttg tgagccgggg ctggaggcgc ggtaaaaggt gcccaccatg
      241 ggggatttaa ccgcgtggcc gctgctgacg ggctcgtctt ttttagccgc taccgtctcg
      301 ctaaccggtg cagctggcgc tgcagcctgg tggacgggag ctgttggcat gggctggtgg
      361 atataggtat gtacgggcgc ggccggcgca cgttggccgc ggctgatgcg cacggattct
      421 tcaccctcgc gaatctctaa ttcttccacg ttcgattctt cgagtagctc aatgagcttt
      481 tttattttgc gaatatccat tgatgcctct taaagtctga gtaaattaat gtgatttgag
      541 gtggctaaat gcagcctcta gcgcgagcag ataactctgt gccccgaacc cacagataat
      601 gcctttggcg atatccgaaa ggtacgagtg gtgacggaag ggctcgcggg cgtgcacgtt
      661 ggacaggtgc acctcaataa agggtatgtg cacgccagcg agagcatccc gcagtgccac
      721 gctggtatgg gtgaatgcgg ctgggttgat gataataaaa tccacccctt cggctttggc
      781 ctggtgaatg cggtcgatta aggcgaactc ggcattgctt tgcaggtgaa aaaattcgtg
      841 ctggtgagat gcgcaggcgg acgccagcag attattgatg tcgctgaggg tggtaaaacc
      901 gtagatttcc ggctcccgtg aacccaacaa atttaaattc ggaccgtgca gaacaagtat
      961 tttggccatg ggacctctta tcaaacctat gcctactgcg ctaactcggc ggttgagcgg
     1021 gccgcttcag cccgaatagg ggcgcaagtc aggattaagt tttcggtaga tgtgaaatgt
     1081 ctgcacaatc gcagaagttc gctgccgatt ggcctgttcg cgctgggcgg ggattttgcc
     1141 taaaagaaaa ggcgctgtct attaaaaaca gcgcctttgg cggagaaact cgtcgattga
     1201 cggcaaaaca gagggggaaa agccgcaggt ttagttagcg gctacttttt tgcctgcagc
     1261 gttgcgctct agggcctcaa tgacgatttc tttggttagt acttgcggca gcaattcagc
     1321 cttgccagat ccagctgggt aaaccacata aagcggcacg ccactgcgct taaactcgct
     1381 caatacggcc gtaattttgg ggtcggcgtt ggtccagtca ccttttaaag tggcatagcc
     1441 caattttttg aaggtgttgc tgacatcttc gcgattgatg gcgttagcct cattggcttt
     1501 acaggtgata caccagtcgg cagtgaaatc cacgaacacg gggcgtcctt cgctgcggtg
     1561 tttttccacc agctcagccg accaagcctc ccagccttca gatttttgtt tgaaggattc
     1621 ggcgttgtag ccgacataac ctgcataaaa cagagcggcg atggcggagc ctttcaccag
     1681 ggttttaccc acagtgcctt cgggctggtt ttgcagcagc cagagtgcaa aggtgatggc
     1741 gataccgccg agcaccaggg ccgccatggc atcaatacct acttggttgc ccaacaccca
     1801 gagtaaccac acgctggtga ggtacatggg gaaggctaag aattgtttta gggtttccat
     1861 ccacatgcct gggcgcggca gcattttggc aagcgctggg ctgtagctca gtgccaggaa
     1921 gggtagggcc aaaccaaaac ccagggcaat gaagataatg atggatacgt accagggctg
     1981 ggtcagcgca aaaccgatag cgggggccat caatgggccg gtacaggggc tggcaactac
     2041 agcggctaaa acaccggtga agaacgaacc gcgcaggcct tccgcagcgg tgaggttttg
     2101 gccaacgccc atcaggctag tgccaaaatg caccatgcct gaaaggctaa gacccatgac
     2161 taaaaacaga taggtgacga aggctacgaa caccggattt tgcaattgga agccccagcc
     2221 cacaccttgc cccgcttggc gcagggccag gattgcgatg gcgactatca cgaaggacag
     2281 gcagacacca aaggtgtacg cccagccgtg gctcgaatgg gagtggccgt cctggctggt
     2341 ggaggcgaag gttaaggcct ttaaagacag taccgggaac acacagggca tcaggttgag
     2401 aatcatgccg ccgccaattg ccagcagtat gtacacgcca aaagagtagg tttcctctgc
     2461 aggagccgcc tcggctgcag tggtggaagc ttgcgcggtg gttgcggcag cagcaggctc
     2521 tgcagcgaca attttggtct ctgctgtttc aagggcggtg ccttggctga ggtcaatttg
     2581 gaagtaacgg gtgtggggcg cgtaacacag gcctgcatcg gcgcagccct gggaggtcag
     2641 tttcatttcg aactgtttgg gcaggttggc cgtggagagg gttatgcgag tggcatcgtg
     2701 gaatatctcc acatcctttt taaagtaggg atcgtatttg ctttggctgg cttcaaacgt
     2761 aggagtgaag ggtgttttgc tctgctcggt ttgagcgaag attttgaatt tttctttgta
     2821 gaggtaatag cctttggcga tggtccaatc caggtaaatg gcattgcctt cctgaattac
     2881 attgacttta taggcctgtt ctacaggtaa aaattcttca gaggtggccg acttcccggt
     2941 tagagagctc acgtcattgg cagcgagtga ctgcaaagaa gtaatgctga ggcaaaatgc
     3001 ccacaggaca aaaaatactg tacgattaat cagtttcata gggatgcgcg ttcgtaagaa
     3061 atatgacaaa acaaggccgg gaacctcact aaaaaggtcc gccgccattg taagttaatc
     3121 ggcaaggctt gcgacggtaa gtttcatcaa tagctaaatc tcggaagcgc tcgcataatc
     3181 aatctgcaaa ttggatcaca gtttttctgc agctttttgg cggggttgcc ttcgttggtg
     3241 tctccgcgtg tcaccggtta caacctgtgc gccaaattct tcgcccagct cccttctctc
     3301 gccatgggtg ggtgtttaga atgtgcgccc ttttatcaag gcttggagtt tttatgagtc
     3361 ggctcgcgcc gctatttgtc ttgcaagtgc tgctgagttt ggccggttgt gttagcgctc
     3421 cagcacagcc tgcgccgccc cctccagtgc aagccaaacc cttggatgcc aaagcgcaga
     3481 agattgagct tttgcttggt cgcgccgata ccgccttgtc catgcaggcg ctgactgaac
     3541 cggcgtcgga aaacgcctac gacctctacc gtgcggtcgt gattatggat ccgactaatg
     3601 tgcgcggaca ggagggcatc aaaaaagtgg ctgaggccta cccggcccag gtggaggagg
     3661 caattgttgg caaacaatat gacgccgctc tgcgcaagct gcaaaagggt ttaatacttt
     3721 ttccaaataa tccgcaatta aaacaaatgg aagcccaggc gaaggccgct aagcttgcgc
     3781 aaacccaagc ggcgtctaaa agcaccacca tcagcctgcc tgcagctgac ctcagcgcca
     3841 aatccccggc agttgagaca accttgcgca acctggctaa gcgggttgcg gtttcaaatg
     3901 aattgttgat tatcaacgcg cgcagcgatg cggagggacg ctggatatat aaaaccatga
     3961 aggacgcagc gggggctcgg ctgcgcggcg atatcaaaat ttcgagcaca ccttcgattc
     4021 agctgcatcc tgccgcgctc tagttaagtg gcttaaggct ctattaataa ataatgcgga
     4081 tattgtgtct gcctctgtgc gcccagtaat gccgattttt acagtggccg cgcctgcaga
     4141 ggtaattcat aaaggtgaat tgagcagcag gttaaaacta tgtgctggct tttaaacagc
     4201 taggcttgtt tgctgccttc gctggttagt tttttgtttt ttgcccattg ataggccttg
     4261 atgttggcgt tatgcaaccc atactggtgg cgcaacgtcg gtataggcca ggctagctgc
     4321 cagtgggtta attggttatg gcgacgccga ctttaatttc attctaattg cagcaggcta
     4381 ggggatgcgc taaacactgg actagacttt ggcggtgtgt gctgtggtca acacatcgac
     4441 tcgatgcgca ggtttcccag cgagtgcgac agcttctgaa cctaaattct ttgcgcgtta
     4501 attgtaaact cccgactcat ttggaataag gcgataatga ctaaattgtt gcagcgaatc
     4561 gcgcttgcgc aaccggtgaa acatgttgtt tggttgttag tgggattggt gattatccat
     4621 caggtattgc cgattaccca ctggcaacag atgttgggtt tgtggtggcg cttgccgttt
     4681 aatgtgattg tgctgatggt gattgccgct gtggccgcct atgggctgct aaatgcctgg
     4741 gattttttgg ccttaaaaaa agaggcgaaa aatcacgctc aaaagcccac atccggcgcg
     4801 ctcctgcagc gcatgattac cgcgcaaatt tgccgcgccc tgggggtggg ggtgcagccg
     4861 gtggtggcgc aggctgggtt tttaccccac gcgggtttat tagttgcggg gttgctggcg
     4921 tgtattggcg ctgtagtggt ggcctcgccg gaattatttg atctgccgtt taaggcccat
     4981 gtgtggttgg cggcgggcat ttttttgctc ggcttgtgtg cgttgtttgt gcgcaccctg
     5041 cgcttgggtt tgggccgcgg cgaaggcgct atggtttccg aattaattaa attagtggtg
     5101 gggcgggagt atcaaaactg gttaaaaccc ctgacgcttt ttttgcccgc agcactcgct
     5161 ttatatatcc cattaaacaa aatcgtgcag atgtccgcgc tgcattttgt gggcatttat
     5221 ttgcttgccc atatggctgg tgtgttgagc cgtgtgcccg gcggggcagg ggtatttgac
     5281 ggtattttcc ttatttttgc cgctaaattt tatccggcca cccaggtgtt tatctgtttg
     5341 gtggcctacc gcgcggtgta ttactgcctg ccgttaatat ccggtttaac ggcttatctg
     5401 ttgttggata ttcgcaaaaa tcaagcgatg agtgttaagc gcccctgggc gctggcacag
     5461 ttgccggggc aattgtttca acccttggta tccctgtggt tactcgcgct gacggttacc
     5521 tattggctgg gccatgtgcc gccgttttta aatggcatta ttccatcgcc gccgagtttt
     5581 gtggcgccct atattaataa tttgttgagt gtaatattcc tgattatttg gcgttgccat
     5641 tggcgcagga ttgatttggc ctatggcctg gtgtttggct gttttatcgt ggccgcagcc
     5701 ttatttgccc tcaatgatgc gttgctaagt agtgttgttt gccttttgat ggtgtggatt
     5761 tttagctcca gcggccatta tttttaccgc accagtgcgc tgctgcaggg tttgattccc
     5821 tgggccttat ggggccgtta ttgtttgggt gggtttttgt tggtgagtgc cacagtgttg
     5881 ctgacggaga ccaatgatat cgaacgcatg tttattagcc agtggatttc cagcgaagta
     5941 ttatcgcccc tggaaaaatt agtctttgta accacctttt ttgccggtgg tttggcggtg
     6001 tatggactct ttacgcgctg cgatgcgcgt ttacagctgc cggataacgc agccttgcag
     6061 cgctaccgcg aactggtgca gcgcgagggc gatacccaat gccatttggg gcttgccggg
     6121 gataagtatt ttttattcag tgccacgggt aaatcctgta ttgcttttgg taaaacgccc
     6181 aaccaatgga ttgcgatggg cgacccgatt ggtgactcca ctgagtttgc cgcgttgctg
     6241 tggagccttg ctgagcaggc ggatgcggcg ggggcaagct tggttttata taaagtcgat
     6301 gcggctaact tgtcttggta tgttgatctg gggatgtcgg cgtttaaatt gggcgatgag
     6361 gcgcgggtaa aactggctga attttccttg gcgggcagta aaaaactcaa tttgcgcaat
     6421 aactacaaca agtgtgtaaa agaaggcttg gaattcgctg tattggaagg cgacagcctg
     6481 aaagcggcaa tgccgcaatt ggcggtaata tctcaggact ggttggacgc taaatccgcc
     6541 cgcgaaaaac aattttcctt ggggtttttc tcggtggatt atttgcagtt tggctcggtg
     6601 gctgtagcta aatatcaagg cgagatagtt gcttttgcca gtttgtggcg ggatataacc
     6661 caacaggagg tggcgattga tttaatgcgc tacggacgca gcgcgcctaa acgcgttatg
     6721 gaatatttaa ccctgagcac tttgttgtgg gcccaagagc agggttatgc ttggtttaat
     6781 ctcggtatgg cgcccttgtc gggtttgcaa tcccaccgtt tggctaatgt gctgcataag
     6841 tttggcggtt atttatttgc aagccatacg cgtttttata atttttctgg ggtgtatacc
     6901 tataaagaaa aatttacccc gcaatggcac ggccgttatt tgattgctaa aaacccctgg
     6961 cagctgagta tcgccttggt aaatgtggcg aagttaatcg ccggtggcgc caagggttta
     7021 attgccaaaa cctagcagaa gttgcggcga tcattttcga tcgctttgat ttccccgtgt
     7081 aacagcagtc tcttttagtg aatgtaaaaa cttttggccg cgctcttaag tggttgcaaa
     7141 gtgttgcagg caaaaactgc acagtgtgta tgccgagtta ctgcggcgtt tcttgaatta
     7201 atttaagccc ccttcgttgg gggttttttg tgagttgagc aagtggtgaa gaaggtaaaa
     7261 ttaggtgtgg gtgggctgct gctggtattg gtagtagcgg cggggttttt tgcggtgcag
     7321 tacttggcgc ctggctttaa tgagtcaatt cacgaacatc acagccaagt ggctggattg
     7381 ggcgatgtgg catttttcaa tgtggatgaa ccggataagg gccacttaat cgtgtttgcc
     7441 gccagcgcag aacccgtaac cgagcggcgc gcattagcgc ggcgttgggc cgataagggc
     7501 tacctagtga gtgtgattga cccaggcttt ttaaaaggcc agtccgcttg tgcagatctt
     7561 gcctccccta tagctcaact tcagcacttt atagaacagc gctaccctca gcttgcgggg
     7621 ttgttgcccc tggtgatggg gcgcggggag gcggcggcgc taagttttat tgccctggcg
     7681 caggcgccgg ccaagtcctt tcacagcgcc attgccgtgg acttttgccc cgcccgagcg
     7741 cttgcgcaaa ccttttgccc ccagcgcgcc tggagcgggc agccaggtgt gatggccgca
     7801 cccagccagg ccttgggagc taatgcgtat gtgtttcaga cacctaggct ggccgcttcc
     7861 ggcacctgtg acctggtggc cagcgagcaa tatcttcagg ctgtgccgtc ggtaaaattg
     7921 agccaggcgc agggtggggc agatgattgg ttagaggtgg atgcgctgtt gaactggctc
     7981 gatccgcgtt tgactagcca ggccttgtcg gcgcaaaacg atgatgactc gccggtggtg
     8041 gaggtgcccg cgcaaagcac ccaaagcgct gattattttg tgctgtttct caccggggat
     8101 ggcggctggg cgcagctgga taaagaattg gcccagcatt ttagtgcgca gggcattaac
     8161 gtgttggggt ttgattcgtt aagttacttt tggcgcaagc gcagcccgga ggaaactgcc
     8221 gccgacatta gtgcgctgat cagcagtta
//
LOCUS       DAOQGS010000127         8228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4138_length_8228_cov_7.246788, whole genome shotgun sequence.
ACCESSION   DAOQGS010000127 DAOQGS010000000
VERSION     DAOQGS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4138_length_8228_cov_7.246788"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKZ52_06505"
     CDS             complement(<1..255)
                     /locus_tag="PKZ52_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59458.1"
                     /translation="MTTLNAFDDVHPEHALRDPRIISRALYADSSARNGKHYLILKDG
                     QHFIFASVYQHINPTINPSGEQWSGGSHIEIPAPALIWLLN"
     gene            complement(505..972)
                     /locus_tag="PKZ52_06510"
     CDS             complement(505..972)
                     /locus_tag="PKZ52_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59459.1"
                     /translation="MIEQLPIPLLSPITCVEQGFVYFGAWHTAPYLLYKSPRYLVIQT
                     QPSLLQHGLSQPSPSQNGPDQLVIPRAAIVWLSHLVRHPFMRREGHTECFEGETLTLR
                     RSAPRDGEGTSGFEIHNTSRHTGNTQRPQRALISDEFMHECLVPFMDGLNSGA"
     gene            complement(1786..2277)
                     /locus_tag="PKZ52_06515"
     CDS             complement(1786..2277)
                     /locus_tag="PKZ52_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59460.1"
                     /translation="MAQPDLISLNIPAADLQDIQAAITTLTTKLGPHLITLSSADRKE
                     IPKMGDKTQAFVGKAREYASQNRDLVPGYLDLAAFEIDWKAVEVLTSLQRQLAPLADN
                     LSDSLALSGSEAYQAALIFYRNVKMAARAGVPNAKTVEADLSSRFPGGSAKASAAAVA
                     NPA"
     gene            2752..3777
                     /locus_tag="PKZ52_06520"
     CDS             2752..3777
                     /locus_tag="PKZ52_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59461.1"
                     /translation="MNMANKDNNLFSRLIKYYYDKTLYAQADKLSALQLSPPQFYKPS
                     ADGSLLLNWACIFSGSFAANAPQEISQVLRACVTQHEETIDNFDFSHIQSLSNSKGFG
                     SNWLAKINGSWFNDAATWGAAMYPDSNLSGLDDQDWAANIRHIEREGFPQSRPITVHY
                     YAWLDRYKAVQPGGSHHCAMVLHQMKEQKRAYTRQATLTRYAINTTTIEQLSRRYCLF
                     VTANTVTDLTLSHNTIDLLTAIYKHISPKACTIPIAPSVNNAVIGCIPYDSLSIDHSI
                     FQRWYAIHVKRGSIVPLVTLLNNTLASCTTPYVHALDSCYLGDPYRKNDIRAKNIPLA
                     TREETAS"
     gene            complement(3877..4392)
                     /locus_tag="PKZ52_06525"
     CDS             complement(3877..4392)
                     /locus_tag="PKZ52_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59462.1"
                     /translation="MRRHPPAHQLQPDLPLIHHHHALPPGVVELAHIHDFSIEGRTWS
                     FLRYQHKLILLMHYRQQSGGDTQYGYFQREFPLEFLRWFPSALAQYEQNPSLAIEGTL
                     GAEQLSLHASDVGRGYCAVLNYSRCQRGHSPITDFRPQETIIANHFIYQGGLLALIKQ
                     LGQQYLPAQPL"
     gene            complement(4501..5013)
                     /locus_tag="PKZ52_06530"
     CDS             complement(4501..5013)
                     /locus_tag="PKZ52_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59463.1"
                     /translation="MPYFVPILPEQVFTDPRICLHFLADDANTQAGRHYLTLLDAGYF
                     IFVIAYQTALTPLNADDPCWQGGTQIELPVSGLGPMLTALEHHYAHTRHHSLRSQAVF
                     KYTKSVADEVLSLSRDYVLPGYALKNHTRKVHLPWAHDDWKQELRLADDLLFTQGYYA
                     ALKQLADDYA"
     gene            5214..5513
                     /locus_tag="PKZ52_06535"
     CDS             5214..5513
                     /locus_tag="PKZ52_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG59464.1"
                     /translation="MSVFALRLKEARTHLGLSQEKLGVLAGIDEASASARMNHYERGK
                     HAPDSTMVERLANVLNVPEAYFYARDADVAWLLIQLHRADKKRKKAIMDYVRKQL"
     gene            5672..6817
                     /locus_tag="PKZ52_06540"
     CDS             5672..6817
                     /locus_tag="PKZ52_06540"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNG59465.1"
                     /translation="MAVAYAHINPANLRWARERAHLSEAALAKVLKVEEQKLIAWEKG
                     LTKLTFKQAQAFAKQTLTPLGYLFLSSAPVEQLPIPDLRTVDNEHNPHPSAELIKMVY
                     TVIERQEWYKEYVRQMSDAQKNPHIGRFTAQTPATQIVADIRTVLNIPAHPERGNWKD
                     YYRNLIKKIEHIGILVMRQGDLGHYSKPLNVKEFRGFALFDPVAPIIFINRADAPSAQ
                     LFTLMHELAHIWIGQSGVSDAKPHTHRAEEILCNAVAAELLVPADEFLSHWQELRNWH
                     ANLPVLEAQFHVSQWVLARRALTLGKITRQQYQSYVDSIQQDYKNRERSEGGPSFYRT
                     RKSQVSERFAKALVSETLSGRVLLRDAGKLLDMAPSKISAFAAELGI"
     gene            6817..7314
                     /locus_tag="PKZ52_06545"
     CDS             6817..7314
                     /locus_tag="PKZ52_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HNG59466.1"
                     /translation="MYLLDANTYIAAHNTYYAPDFCPAYWDWLDNQFSQGHVFSVNMV
                     YQELAGKNDALSKWVKARKKHFLPENDDATQEHFKRIMEYVYALPHKNPANVASFAEG
                     ADPWLLAKALTVGATVVTQERLAPGNSFKIKIPNVCQHYTIDYIDSFQLLKNLRARFI
                     IDSRS"
     gene            complement(7456..8115)
                     /locus_tag="PKZ52_06550"
     CDS             complement(7456..8115)
                     /locus_tag="PKZ52_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59467.1"
                     /translation="MIYSSVEDNWLRIELMFEIASKYNDFTKGEDGFDVAETDGKLSF
                     RMDVFLMDGEKKESCLVGMLMVWSVVTLESLINHAIAESINNELTAKLAIEHPGQITE
                     KIKPRVNAKSELAKKLLILNDDSEDISKLLEIAEQLSERRNHIVHDKPFDHCTDEDGE
                     PIIKYFRVRGKELDERIRFKSLGPFFKQCDSVKEFIQRKSNLGSLDVHGKSFSGLLND
                     V"
BASE COUNT         2031 a         1999 c         2116 g         2082 t
ORIGIN      
        1 gttaagcaac cagatcagtg ccggggctgg tatttcaatg tggctgccgc cgctccactg
       61 ctcaccgctg gggttaatgg tggggttgat gtgttgatag acgctggcaa aaataaaatg
      121 ctggccgtct tttaaaatta aatagtgttt gccgttacgg gcgctagagt ctgcgtatag
      181 agcgcgggaa ataatgcgcg ggtcgcgcag ggcgtgttca gggtgtacat catcaaaagc
      241 gttgagtgtg gtcatggcgt cctcgcgtgt cctggtaatg gttcagtagg tgggtacatc
      301 gcttttttga tcgtttggta tttttgtgcg ctgtgttttt tcgtcctctt tgttctgcgc
      361 gatttttctc ctctttttta tttattttta cttttatttt taattttttg ttttgcctta
      421 attttcctgg gtcactcgtt tggcccggtt taccaccccg ccccgttggg gcacccctcc
      481 agcggagggg aatttttaag gtattcaggc gccactgttt aatccatcca taaacggcac
      541 taaacattcg tgcataaatt catcgctaat cagggcgcgc tgtgggcgct gggtgttgcc
      601 ggtgtggcgg ctggtgttgt ggatttcaaa cccactggtg ccttcgccat cgcggggggc
      661 gctgcggcgc agggtgaggg tttccccttc gaaacattcg gtgtggcctt cgcgacgcat
      721 aaaggggtgg cgcaccaaat ggcttagcca aacaatggcc gcgcggggga tgactaattg
      781 gtcggggcca ttctgcgacg ggctgggctg cgacaggccg tgctgcaaca ggcttggttg
      841 cgtttgaatc accaggtaac gagggctttt atacagcagg taaggcgctg tgtgccaggc
      901 accaaagtac acaaaccctt gctcaacaca ggtaatgggg cttaggaggg gaatggggag
      961 ctgttcaatc atgcagtgcc ctgcggcatt aaacgatgac ggtaaaaagg tgcgtgcggg
     1021 tgtgctgggg gcatggtgca tccttgcgtg tatgggctgc ggcccggtga ggttgcgccc
     1081 atggcgatgt ggtcattacg cctggggcgg cattatgggc agggacaggc gggttttata
     1141 ggggcgatat gcggcatcgg gtgtgcgata tatctcacag tttttgcccg cgccagggcg
     1201 gtgttgtgca ccgctagtgc gtgccatggg cactctgtat gcctctcctg tggagtgtgc
     1261 ccatgcttat atacggtgcc aaattgcttg aagcgccaat tcccctcctg tggaggggtg
     1321 cctcgtagtg gcggggtggt tgctcgcccc gccgctctgg gggctttgca ccagggcatt
     1381 gagcctttta cctgcggcgc cgagtgattg aagaccctcg ccgaggcttt gaggtgcgac
     1441 gccgagtgtt tgaacagcct cgccgcatct tttaagtgcg gttttgtatg aaaaaacctc
     1501 gctgccgcac ggtttaggta cggcaccgaa tggttctaca cccctgccgc atcttttagg
     1561 ttcaatgccg caggtcggca caccctcaag gctgtagccc agtggctggg gggtgtagcg
     1621 aagaggcgtg agggggtggc gcagtaggtg aagggtgttc cgaaggggct gaagggggtt
     1681 gcgaagtagg gggtgggggg tgcagctagg cgcgcggtaa tgggctgaaa cagcaaggca
     1741 aagggggtgc cccctgccct gcatcaaccc ccgcaggggc tttgtttacg cggggttagc
     1801 cactgcagct gcgctggctt tagcgctgcc acctgggaag cggctggaga ggtctgcttc
     1861 aacggttttg gcgttaggta ctccggcacg ggcagccatt ttgacattgc ggtaaaaaat
     1921 cagcgcggct tggtaggctt cgctgccact taaggccagg ctgtcgctga ggttatccgc
     1981 cagcggggcc agttggcgct gcaagctggt cagcacttct accgctttcc agtcaatttc
     2041 aaaggcggcg aggtcgaggt aacccggtac aaggtcgcgg ttttggctgg catattcccg
     2101 cgctttgcct acaaaggcct gggttttatc ccccattttg gggatttcct tacggtcggc
     2161 gctgctgagg gtaatgaggt gggggccaag tttggtggta agggtggtga tggcggcttg
     2221 aatgtcttgc aagtcggcag cggggatgtt caatgagatc aaatcgggtt gggccatgat
     2281 ggggttcctt aaaaaataag tgagcgtggt gtaacgcggt ctgccctttc agcccatgga
     2341 ttgagttgat agggcacggc tattatcacg gagcttaagg gcgctgtgca ggcgatatag
     2401 ccgcctctga atgtaggcta tatcgcacac gcagggggtg caggtggcaa cggggcagag
     2461 tccagcggat gaggcgcccg gtttaaacac aagggtttta acaagaacgc cgcgtgcaca
     2521 ccgctagctt ataacgccct ttttatcgcg attgctcaaa ctgattcgcc gtcacactta
     2581 gcaactggat ttttctcggc ttggctatac aacccaacca ctcggcacta aagtgcagcg
     2641 cataaaaata aatgggcaat atagcccgca atagacctca gaaatcagat tggtctttat
     2701 agttaaaaaa ttcaacgcat ttttttattt taatttgcca ggagcaaagc cgtgaatatg
     2761 gccaataaag ataacaatct attttcaaga ctaatcaagt attactacga caaaacactt
     2821 tacgcgcaag cggataaatt atccgccttg caactgtcac caccacagtt ttacaaaccc
     2881 agtgcagatg ggagcctgtt gttaaactgg gcgtgcatat tttccggtag ttttgccgcg
     2941 aatgcccccc aagagatatc gcaagtatta cgggcctgcg tcacccagca cgaggaaacc
     3001 atagataatt ttgatttttc tcatattcaa agcctcagca attcaaaagg gtttggcagc
     3061 aattggctgg cgaagattaa cggcagttgg tttaacgatg ccgccacctg gggtgccgcc
     3121 atgtatcccg atagcaatct ctccggccta gacgatcagg attgggctgc gaatatccgg
     3181 catattgaac gggaagggtt tccacaatcc aggcctatta cggttcatta ttatgcttgg
     3241 cttgatcgct acaaggcggt gcagccgggt ggatcgcatc actgcgcgat ggtgcttcat
     3301 caaatgaagg agcaaaaacg cgcctacact cgccaagcga cgctcacacg ttacgccatt
     3361 aataccacca ctattgaaca attatcgcgg cgctattgcc tattcgttac agccaacacc
     3421 gtcactgact taaccctctc ccataacaca attgatttat taacggctat ttataagcat
     3481 atatcgccaa aagcctgcac cattcccatt gccccaagtg ttaataacgc agttattggc
     3541 tgtatcccct atgactcatt aagcattgac cattctatat tccagcgctg gtacgctata
     3601 cacgttaaaa ggggttctat cgttcccctt gtgacactcc tcaacaacac attggcatcg
     3661 tgcacaacgc cttatgtaca cgcgctggat agctgctact taggcgatcc ctatagaaaa
     3721 aatgatatac gcgcaaaaaa tatcccactc gccacacgag aggaaacagc ttcataagcg
     3781 cgtcatttat tttttatttt tagctaatcg gtttcgttta ttgcctcgcg aaatgtcctt
     3841 aagcggtcgc cattcatctc tatctgtacc gcttttttat aagggctgcg caggtaaata
     3901 ctgttggccc aattgtttaa ttaacgccaa taacccgcct tgataaataa aatggttcgc
     3961 gatgatggtt tcttgcggcc taaagtcggt tatggggcta tggccgcgct ggcaacggga
     4021 ataattcaat accgcgcaat aacctcttcc tacatcgctc gcgtgcaggc tgagctgttc
     4081 tgcccctagc gtgccttcaa tggcaaggct agggttttgt tcatattgcg ccaacgcgct
     4141 gggaaaccag cgtaaaaatt ccaagggaaa ttcccgctga aaatagccgt attgcgtgtc
     4201 accgccggat tgttggcggt agtgcattaa taaaataagt ttgtgttgat agcgtaaaaa
     4261 agaccaggtg cggccttcaa tactaaaatc gtgaatgtgt gccagctcca ccactcccgg
     4321 cggcaacgca tggtgatgat ggataagcgg taaatccggt tgtaactggt gagcgggcgg
     4381 gtggcgtctc atgatgtgct cctggggtaa gtccgtgggt taatgctgtt tattaaaggt
     4441 gtttagcgct ctgtttaggc cgtgagcttg gtctgtgacc accgaaaagt gcctagcctg
     4501 ttaggcataa tcatccgcca attgttttaa cgcggcgtaa tagccctgcg taaataacaa
     4561 gtcatccgca agccgcaact cctgcttcca atcgtcatgc gcccaaggta agtggacttt
     4621 gcgggtatga tttttaaggg cgtaaccggg cagcacatag tcgcggctta ggctcagcac
     4681 ttcatcagca acgcttttgg tgtatttgaa aacggcttgg ctgcgcaggc tgtggtggcg
     4741 ggtgtgtgcg tagtggtgtt caagggcggt aagcattggg ccaaggccgc tcaccggcaa
     4801 ctctatttgg gtgccgccct gccaacaggg gtcatctgcg tttagaggcg tgagagcggt
     4861 ttgataggcg ataacaaaaa taaagtagcc tgcatccaac agggttaggt aatgccgacc
     4921 ggcttgggtg ttggcgtcat ccgccaggaa gtgcaagcag atgcgtggat cggtaaaaac
     4981 ctgttcgggt aggatcggta caaaatacgg cacgttagtg attactcctt gtttatcacg
     5041 gcggtttagg ccttgggcgt aggggctgct atgtgcgcga agccacctaa cgggctaccc
     5101 gcccctcgac aatggcggcg ggccgcagcg gcgcacatca ctaagtattc cttagtgtga
     5161 ggcacagtat aacctaagaa tatcttagcc ttcatcacta ttgagcagtt gggatgtcgg
     5221 tttttgcgtt acgcctaaaa gaagcccgca cacaccttgg tttatcgcaa gagaaactgg
     5281 gggtattggc gggcattgat gaggcgtccg ccagtgcccg catgaaccac tacgagcgag
     5341 gcaaacacgc accggacagc acaatggtgg agcgccttgc caacgtgctg aatgtacccg
     5401 aggcgtattt ttatgcccgc gatgcggatg tggcgtggct gttaattcag ctgcatcgag
     5461 cggataaaaa acgtaagaaa gcgattatgg attatgtgcg gaagcaactt taggacgtca
     5521 ggattaacat cttagcaacg aggctgatgt gtaggtgtaa tcacaggcaa cttgccaggt
     5581 cggaactctg cactgtgtta actcaagtca catcgctata cttcatccac tacatgttca
     5641 ctcgcaccct accgattgca gacatatatt tatggccgtc gcatacgcgc atatcaatcc
     5701 cgctaacctt cgctgggcgc gcgaacgcgc ccacttaagt gaggcagctt tagccaaagt
     5761 gctcaaagtg gaggaacaaa aactaattgc ctgggaaaaa gggctcacca agctcacatt
     5821 caagcaggcg caagcctttg ccaaacaaac actcacacct ttagggtatt tatttttaag
     5881 ttccgcaccc gttgagcagc tgcccattcc tgatttgcgc acggttgata atgagcacaa
     5941 cccacacccc agtgccgaat taattaagat ggtctacacc gtcatcgaac ggcaagagtg
     6001 gtataaagaa tacgtgcgcc agatgtccga tgcgcaaaaa aacccacaca ttggccgctt
     6061 cacggcccaa acacccgcaa cccaaattgt tgccgatata cgtaccgttc tgaatatacc
     6121 ggcacatcca gagcgcggta actggaaaga ctactaccgc aatttgatca aaaaaataga
     6181 acacataggc atactggtca tgcgccaagg tgacctcgga cattacagta aaccgctcaa
     6241 cgttaaagaa tttcgtgggt tcgcactatt tgatccagtg gcacccatta tatttattaa
     6301 cagggccgat gcgcctagcg cacagttatt caccctgatg catgagctag cgcatatttg
     6361 gattggccaa agcggtgtct ctgatgcaaa accccacacc catagagcag aagaaattct
     6421 atgcaatgcg gtagcggcgg agctgttagt gcctgcagat gaatttttga gtcattggca
     6481 agagctgcgc aactggcacg ctaatttgcc tgtactagag gcgcaattcc acgttagcca
     6541 atgggtcttg gcacgccgag ccttaacact cggcaagatt actcggcagc agtaccaaag
     6601 ttacgttgat agtattcaac aggactataa aaaccgtgag cgctccgagg gtggcccgtc
     6661 tttttaccgc acacgcaaga gccaagtcag cgagcgcttt gccaaggctt tggtatcaga
     6721 aacattaagc gggcgagtct tgttaagaga cgcgggaaaa ttattagata tggcacccag
     6781 taaaatcagt gcctttgcag cggagttagg gatctaatgt acttactgga cgccaacacc
     6841 tatattgccg cgcacaacac ttattacgcc cctgattttt gtccagccta ttgggattgg
     6901 ttagataacc aattttcgca ggggcacgtt ttcagtgtaa atatggttta ccaagaatta
     6961 gccgggaaaa atgacgccct aagtaaatgg gtcaaagcgc gcaagaaaca cttcctgccg
     7021 gaaaatgatg atgcaaccca ggaacatttt aaacgcatta tggagtacgt ttatgcactc
     7081 cctcataaaa atccggcgaa tgttgcaagc ttcgcagagg gtgctgaccc ttggttactc
     7141 gcaaaagcac taaccgttgg cgccaccgtt gtaacccaag agcgactggc tccaggcaac
     7201 agctttaaaa taaaaatccc caacgtatgc cagcattata ctatcgacta tatcgacagt
     7261 ttccagctac tcaaaaatct gcgtgcgcgc tttattattg acagcagaag ctaatattga
     7321 aaatgtatta gatcagatta ttaactgcgc aataataatt ctctacaatg ccttaaggca
     7381 aaactccaac attagatcag cagaaaatac aatcgaaata cagcggcagc gagtgatttt
     7441 tactattgat ttcagctaaa cgtcattcaa cagcccagaa aaactctttc catgcacatc
     7501 aagagaacca agattggact tgcgttgtat aaactccttg actgaatcac actgcttaaa
     7561 aaacggcccc aggcttttaa atctgatcct ctcatccaat tccttacctc ttaccctgaa
     7621 atatttaatt atcggctctc catcctcatc ggtgcaatga tcaaaaggtt tatcatgcac
     7681 aatatgattt cgacgctcag acagctgttc cgcaatttcc aatagcttgg atatatcctc
     7741 tgaatcatca ttgagaataa gtagcttctt agccaactct gacttagcat tcactctagg
     7801 cttaattttt tctgttatct ggccaggatg ttcaatggcg agctttgctg tcaattcatt
     7861 atttatactt tcagcaatag cgtgatttat aagtgattcc aaggtcacga cagaccacac
     7921 cataagcatg ccaacaagac aggattcttt cttctccccg tccattaaga agacatccat
     7981 acgaaaactg agcttgccgt cagtttcagc aacatcaaaa ccatcttcac cttttgtaaa
     8041 atcattatat tttgaagcaa tttcaaacat tagctcgatt ctgagccagt tgtcctcaac
     8101 cgaactataa atcacaacct attctcctta ttgatacatc gccaccagca ataaaagcac
     8161 aagccctaaa ggcacatact cattaaagtt gccgtgcacg cggatggccc gctgcaataa
     8221 ctcctgct
//
LOCUS       DAOQGS010000128         8171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4173_length_8171_cov_4.141203, whole genome shotgun sequence.
ACCESSION   DAOQGS010000128 DAOQGS010000000
VERSION     DAOQGS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_8171_cov_4.141203"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKZ52_06555"
     CDS             <1..223
                     /locus_tag="PKZ52_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59468.1"
                     /translation="PIQANLIYQFNPRVGFERVGFSGSSVTFSGESLLAIGAGDTEGD
                     SAKILKRVFRQALQHKLGPKPLKSRDYFR"
     gene            complement(357..1010)
                     /gene="purN"
                     /locus_tag="PKZ52_06560"
     CDS             complement(357..1010)
                     /gene="purN"
                     /locus_tag="PKZ52_06560"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNG59469.1"
                     /translation="MGGKIVVLISGGGSNLQSLIDACARSHIDGTICAVISNRPDVKG
                     LERAHKAQIPHQVIDHTGFANRQAFEAALSEAINHYAPDLIVLAGFMRVLTEGFVNSY
                     LGKLINIHPSLLPKFTGLHTHQRAIEAKETHHGASVHFVTAQLDGGPVIIQDRVEIDP
                     QDDATSLAAKVLVKEHQIYPQAVQWFMSGELAFKEGKCFLHNQPLGPEGIQVWLHRL"
     gene            complement(1010..2053)
                     /gene="purM"
                     /locus_tag="PKZ52_06565"
     CDS             complement(1010..2053)
                     /gene="purM"
                     /locus_tag="PKZ52_06565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNG59470.1"
                     /translation="MSQPTSPSLSYKDAGVDIDAGNRLVDRIKTVARKTARPEVLAGL
                     GGFGALFELPKGYQEPVLVSGTDGVGTKLRLAIQLKKHDSIGIDLVAMCVNDLIVGGA
                     EPLFFLDYYATGKLDVDTAAAVVSGIGEGCLQAGCSLVGGETAEMPGMYEGEDYDLAG
                     FCVGIVEKSKILDGSKVSAGNAILGLASSGPHSNGYSLIRKILEVSQAELTQPIGATT
                     LADALLAPTRIYVKSLLKLLKTYPVHAMAHITGGGLLENIPRVMPPRTKALINSNRWE
                     MPEVFSWLQKSGNVQMEEMYRTFNCGIGMVIIVPVDVADAASELLTREGETVFNLGQV
                     QTASNEAPFVEII"
     gene            2629..2880
                     /locus_tag="PKZ52_06570"
     CDS             2629..2880
                     /locus_tag="PKZ52_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59471.1"
                     /translation="MEKHATAYSGYGGETSGKVWSLEEDKYCRFVVWVLEDNYKYMNL
                     KDKNNWHGGCYSWLKVEDQFGTLTQGRDEILKIGEALCL"
     gene            2871..5021
                     /locus_tag="PKZ52_06575"
     CDS             2871..5021
                     /locus_tag="PKZ52_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNG59472.1"
                     /translation="MPLVINTNVSSLNAQRQLVSSGNELSTAMERLSSGKRINTAADD
                     AAGLAISNRMTSQIRGLNQAVRNANDGISLIQTAEGALDETTNILQRMRELAIQSANG
                     IYDDSNRATLDAEVQQLIAELDRIAKTTTFNGQTLLDGSLGDVQLQVGSQANQTIDIA
                     IQAMDSKTLGMGSVSVDVLGAAVTANLSTVTLNETDVLINGQSIGSFTGSIDTFQDLI
                     DNINKNVLGVEASGYTNLKGDLVGNGVLENGNKLRITVQAADGSGNNVFNVTDTKNIN
                     ELIEKIVTVTGGIVSADKDEAGHLVLTNNAGSTIVVDAIDTTGAVTDAASQLVEQATG
                     FNVFDPTLAINSQGITQSAEGQVILTSKHGDPITVSRGATGTTADLLNLGFRESFKAG
                     TVTGVGIDSAHAATAWNVGDIKINGTIIDNDNTDSLQGKINAINASSFDTGVVANAFA
                     TASIDLSTANLSLASGSSDNLVINGVVIDLGLASGATASDVVGGFNLNSDLTGVHARL
                     SGTRIILESDQGSINLAAYSANSAFGSTAALFGATIASDVADSTGSLTSSSITASSTF
                     TLTVEAGIKLTSTNQSPISIELGVNANPASLGLMEANSTAGGRFGVAIANVSIDTAAH
                     AQKAIGVIDKALESINNVRSQLGAANNRLDFTINNLLVVSENTSSAKSRIMDADFAAE
                     TSALSRAQVLQQASQAMLAQANASSNQVLQLLRQ"
     gene            5098..6456
                     /locus_tag="PKZ52_06580"
     CDS             5098..6456
                     /locus_tag="PKZ52_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNG59473.1"
                     /translation="MLSPLLKTTANQTQPALPGEHQALHKSRNEQDFNQIFNQQREQP
                     VRERSDSGNGSQVKKDVKSPTQSSGQSAERADADAPSDSATVSNLHLKDQSGQPVDAV
                     EHEDAEVDIQNVSQYTDELKIDANTLEQNSSWLFWLHYAQDASVVEKPLEVSTLQQPN
                     VPVGLAKNEPQTLALAPLASMPFEASVADAEQPMGTIAAELAPIAANTKELNAPISAL
                     NKTNENKALNDVVNGFTSPVDSTEDVGFTSLLAERVPNVLVKPQADVAVQSRELPLLG
                     EASVDSARIPAHQTVVQENQWLGQAQQPGHAVASTALDNKSFLQLRFNQQTLATDLVE
                     KTGWLIEHKVDTAQIQLDPPELGPIAVKIHTHQEQVSVSFVVANPQVRDAMDQTLQRL
                     KDLLQEQGINLSHADVNDQRQQRDNSGEDAPSQSSHGESTDEEIIGIHLPQDELGVDH
                     FV"
     gene            7108..>8171
                     /locus_tag="PKZ52_06585"
     CDS             7108..>8171
                     /locus_tag="PKZ52_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNG59474.1"
                     /translation="MNNLKSLVLWGAVALLGAWAFAVLALKQGETVNALWIVVAAVCV
                     YLIAYRFYAQFIGKKVMQLDDNRPTPATIRNDGLDYVPTHKWVLFGHHFAAIAGAGPL
                     VGPILAAQMGYLPGTLWILVGVVLGGAVQDALVLFASSRRDGRSLGEMIRQEMGPAAG
                     AIGMLGILAIVIILLAVLGMVVVKALAHSPWGLFTIAATIPIALFMGIYSRYWRPGKV
                     GEVSFIGFILLMAALIGGEWVSHQPFAQEYLSLSAEALALGLMGYGFIAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLALGIVITAPELKMPAITQFIDGSGPVFAGSLFPFLFITIA
                     CGAISGFHSLVASGTTPKMLA"
BASE COUNT         2032 a         1846 c         2104 g         2189 t
ORIGIN      
        1 gcccattcag gcaaatttaa tctatcaatt taacccccgc gtggggtttg agcgcgtggg
       61 ttttagcggc tcatcggtta ctttttctgg tgagagcctg ttggctatag gtgcaggtga
      121 tactgaaggc gacagcgcaa aaattctcaa gcgtgtattt cgtcaggcgc tgcagcacaa
      181 gctggggcca aagcccctta aaagccgcga ttattttagg taggccaact aacaacaggc
      241 gattactgca cagcgtatat actaaagtga atgttccgca ttaccccggt tctttcgcct
      301 ttgtttcgtg gtgctggtga acacaacatc caaaccagta tgcgtattaa cagaatttat
      361 aatcggtgca accacacctg tataccctct gggcctaaag gctgattgtg taaaaagcat
      421 tttccctctt taaatgccaa ttccccactc ataaaccatt gtacagcttg cggataaatt
      481 tggtgttcct taactaaaac cttagcagcg aggcttgttg catcgtcttg tgggtctatt
      541 tctactcggt cttgaataat aaccgggcca ccatcaagct gcgccgtgac aaaatgaacg
      601 ctcgcgccat gatgagtttc cttggcctca atggctcgtt ggtgggtgtg aagaccggta
      661 aatttaggta ataaagacgg gtgtatattg attaatttgc ccaggtagga attgacaaag
      721 ccctccgtta atacacgcat gaaaccggcc agtacgatta aatccggtgc gtagtgatta
      781 atggcctcgc tgagggctgc ctcaaacgct tgccgattgg caaagcctgt gtggtctata
      841 acttgatggg ggatctgcgc cttgtgcgcg cgctccaaac ctttaacatc cgggcggttg
      901 ctgatgacag cgcaaatagt gccatcgatg tgcgatctgg cgcaggcgtc aataagtgat
      961 tgtaaattac tgccgccgcc ggaaattaat acaacaattt tgccgcccat taaataattt
     1021 ccacaaaggg cgcttcatta gaggcggttt gcacctggcc taaattaaat accgtttcgc
     1081 cttcgcgggt taacaactcg ctggctgcat cagccacatc aacgggcaca ataataacca
     1141 tgccgatacc gcagttaaat gtgcggtaca tttcttccat ttgcacattg ccgctttttt
     1201 gcagccagct aaatacttcg ggcatttccc agcgattgct gttgatcagc gccttggtgc
     1261 gcggcggcat aacgcgcgga atattttcca acaaaccgcc accggtaata tgtgccatgg
     1321 cgtgcacggg ataggttttt aacagtttta acagagattt aacgtaaata cgcgtgggcg
     1381 ccaaaagtgc atcggctaag gtggttgcgc ctatgggttg ggttaattcg gcttggctca
     1441 cttcaagaat tttgcgaatt aacgaataac cattggagtg tgggccgctt gaggcgagac
     1501 ctaaaatggc attccccgcc gatactttgc tgccatctaa aattttagat ttttccacaa
     1561 tgccgacgca gaaaccggct aaatcgtaat cctcaccttc atacatgccg ggcatttcag
     1621 cggtttcgcc gccgactaac gaacaaccag cctgtaaaca tccttcccct ataccgctca
     1681 ccaccgccgc agcggtatcc acgtccaatt tgcccgtggc gtaataatcg aggaaaaaca
     1741 gcggttcggc gcccccgaca ataagatcat tcacgcacat ggcgactagg tcgataccta
     1801 tgctgtcgtg ttttttcaat tgtatggcta ggcgcagctt ggtgcccact ccatcggtac
     1861 cagagactaa gacaggttct tgataaccct tggggagttc aaacagagcg ccgaagccgc
     1921 cgagccctgc gagcacctcg gggcgagcag ttttgcgcgc aacggttttg atgcgatcga
     1981 ccagcctgtt gcccgcatca atatcgacgc ctgcatcttt ataacttagg gagggggagg
     2041 tgggttggct catagggttc tcgttcgatc tcaggtgcaa ggcgcatcag cgcgaaaagg
     2101 acggcatttt aacagcttag gggccctttt agtacttttg gccccgctta aaaatacgcc
     2161 atttgcactg ggcggttggg aatttttggg cgttagaagg aagaatgggc gctacaggct
     2221 ccttttgggc aaggcggcag gtttcgatgc aaagcctggc ttaacttaaa tagtgcccag
     2281 ggatgagcag ggcagccttt cactttagtc gcctgttgaa atatctgggc cgcaaaggac
     2341 tgggacggaa tatcggcgct gggggttcga agaggttgcc taaacgccga gttttatacg
     2401 cagtaaacaa agatatttca ccgaatttaa gagggagttt ggggtgcgga gtagaagata
     2461 ggtgaaaagt cgttttctat tttgattcgg ttttccacac tgacggtttc ggataacaac
     2521 cgcaaataaa gttcggaggt ggaaatattt tataactacc cggctatccg cgcgcgagtt
     2581 ttgaaagcag ccatcatccg tgatccccaa gttcccaatt ggtgaatagt ggaaaaacac
     2641 gcaactgcat attctggtta tggtggggaa acaagcggca aggtttggag tttggaggag
     2701 gataaatatt gccgctttgt tgtttgggtt ttagaagata attataagta tatgaattta
     2761 aaggataaaa ataattggca tggcggttgc tattcttggc ttaaggtcga agatcagttt
     2821 ggaacattaa cccaaggtcg cgacgagatt ttaaaaatag gagaagcgct atgcctctag
     2881 tcattaatac caacgtgtcg tccttgaatg ctcagcgtca gttggtgagt tccggtaatg
     2941 aattatcaac tgccatggag cgtttatcat ccggtaaacg aattaatacc gcagcggatg
     3001 acgcagcagg cctggcgatt tcaaatcgaa tgacctcgca aattcgcggc ttaaatcagg
     3061 cggttcgcaa cgccaatgac ggcatttcac tcatccaaac tgccgaaggt gcgttggatg
     3121 aaactaccaa tattttgcag cggatgcgcg aattggctat tcaatccgcc aacggcattt
     3181 acgatgattc caaccgggca actttggatg cagaggttca gcagttgatt gctgaattag
     3241 atcgcattgc taaaaccact acatttaacg ggcaaacact gttagatggc agtttaggtg
     3301 atgtgcaatt gcaggtgggt tcacaggcca atcaaaccat tgatattgcc attcaggcga
     3361 tggactccaa aacattaggc atgggttcgg ttagtgtcga tgtgttgggt gcggcggtaa
     3421 cggctaattt atccactgta accctcaatg aaaccgatgt gcttataaat ggccaaagca
     3481 ttggttcgtt taccggttca attgatacct tccaagattt aattgataac atcaataaaa
     3541 atgtcttggg tgtagaggca tcaggttaca ccaatttaaa aggtgaccta gtaggcaatg
     3601 gggtgttaga aaacggcaat aaattgcgca tcacggtgca agcagccgat ggtagcggca
     3661 ataacgtctt taatgttacc gatactaaaa atatcaacga gcttattgag aaaatagtta
     3721 cggttaccgg cggtattgtc agtgccgata aggatgaagc tggccatttg gtgcttacca
     3781 ataatgccgg ttcaaccatt gtggtggatg ctattgatac tacgggtgct gtgactgacg
     3841 cagcttccca gttggttgaa caagcgacgg gctttaacgt atttgatccg acgctcgcga
     3901 ttaactccca aggcattact cagtccgcag aggggcaggt tattctcacc agtaaacatg
     3961 gcgatccgat aacggtaagc cgtggggcca cgggcactac tgctgattta ttaaatttag
     4021 gatttcgtga atcctttaaa gcgggcacgg ttactggggt gggtattgat tctgcccacg
     4081 ccgccaccgc ttggaatgtg ggcgatatca aaattaatgg cacaattatc gataatgaca
     4141 ataccgatag tttgcaggga aaaattaatg ctattaatgc cagcagtttt gatacgggcg
     4201 tggtggccaa tgcatttgct accgcctcca ttgatttaag caccgcgaat ttaagtctcg
     4261 cctcgggctc ttcggataat ttagtcatca atggtgttgt gattgattta ggccttgcca
     4321 gtggtgccac cgcttctgat gtggtgggtg gatttaacct caattccgat ttaaccggtg
     4381 ttcatgcgcg cttatccggt acgcgcatta ttttagagtc ggatcagggg tcaattaatt
     4441 tagcggctta ctctgcaaat agtgcatttg gctccactgc ggctttattt ggcgccacta
     4501 ttgcgtctga tgtggcagat tctacaggct cattaacgag ctcaagcatc acggcaagtt
     4561 ctacatttac gctcacagtt gaggcgggta ttaaactcac cagtaccaat caaagcccaa
     4621 tcagtattga actgggcgta aatgctaacc cagcgtcttt agggcttatg gaggctaatt
     4681 caaccgcggg cggacgattt ggtgtggcta ttgccaatgt gagtattgat actgcggccc
     4741 atgcgcaaaa agccataggt gtgattgata aggcattgga gtccattaac aacgtgcgct
     4801 cgcaattggg tgcggctaat aataggcttg attttaccat caataattta ttagtggtgt
     4861 cagaaaatac ctcgtcggca aaatcacgta ttatggatgc tgacttcgcg gcggaaacat
     4921 cggcattaag tagagcgcaa gtattgcagc aagcatcaca agcgatgttg gcgcaagcga
     4981 atgcatcgtc gaatcaggtg ttgcaactgt tacgtcaata gtgtgttgtt gcttcaataa
     5041 tggatttaat aaggactggc tcaatcttga atcgtgtaaa gtttatttgg atttattatg
     5101 ctatcgccgc tgcttaaaac tactgccaat caaactcagc cagccttgcc gggtgagcat
     5161 caagcactcc ataaaagtcg caatgagcag gattttaacc agatatttaa ccaacagcgt
     5221 gagcaacctg tgcgggagcg tagcgatagc ggtaacggta gccaagtaaa aaaagatgta
     5281 aaaagcccta cgcaaagctc cggtcaatcc gccgagcgcg cagatgcaga tgcgccatct
     5341 gattctgcta ctgtctcgaa tttgcattta aaagatcaat cagggcagcc agtagatgcg
     5401 gttgaacacg aggatgcaga agtcgatata cagaatgtaa gtcaatacac ggatgaatta
     5461 aaaatcgatg ctaatacgct tgagcaaaac agtagttggt tattttggtt gcactatgcg
     5521 caggatgctt cagttgttga aaaaccgctg gaggtgagca cactgcaaca acctaatgta
     5581 ccagtaggct tagctaagaa tgagccacaa accctcgcgc tggcaccact ggcgtctatg
     5641 cctttcgagg cttctgttgc cgatgcggag caaccgatgg gcactattgc ggcggaatta
     5701 gcgcccatag cggcaaacac caaggaattg aatgcgccga taagcgcttt gaataaaaca
     5761 aatgaaaata aagcattaaa tgatgtggtg aacggtttta cctctcctgt tgattcgacg
     5821 gaagatgtag gttttacctc gctgctagca gagcgtgtac ctaatgtact ggttaaacct
     5881 caggcggatg tggccgtgca gtctcgtgaa ttgcctttat taggcgaagc gtcggtagac
     5941 tctgcgcgta tacctgcgca tcaaacggtt gttcaggaga atcagtggct ggggcaggct
     6001 cagcagccag ggcatgcagt tgcgtcaacc gcgcttgata ataaatcatt tttgcaactg
     6061 cgctttaatc aacaaactct tgccactgat ttggtggaaa aaaccggttg gttaattgag
     6121 cacaaggtgg atacggcgca aatccagtta gatccaccgg agcttggtcc gattgctgtc
     6181 aaaattcaca cccatcaaga gcaggtgagt gtttcatttg ttgtcgctaa cccccaggtg
     6241 cgcgatgcca tggatcaaac cctacaacgc ctaaaggact tattacagga gcagggcatt
     6301 aatctttccc acgctgatgt aaatgatcag cgccagcaaa gggataattc cggcgaggat
     6361 gcgccatccc aaagcagcca cggggagtca acggatgaag aaattatcgg cattcatctt
     6421 cctcaggatg agttgggtgt agatcatttt gtatagcacc tggccctgca gccgccatta
     6481 aaaaccccct cgttatcgcc accttatttc aacacgccgc taaagcaaaa atacttattg
     6541 ctgcgctaac ggcttttctc cctttctcgt tttggcccgc tcattgccca gcagcttgat
     6601 gaaatactgc cgtgccagcc cggcgccgcc taggtatttc aacaagctgc tagttcagtt
     6661 gtggcggtat gggcttttct tttgggtaaa aactgtgttg tgccctacag cgcatcttgt
     6721 gttggccggt attgtgctaa gccagttggg aaattgattt aggagggtca agctgcgctc
     6781 ttgaaaccgc ctattaatca gtggcttctt taattggtgc tcggtggctg ctgagtattt
     6841 tgcagtcatg ggtataaaag cttcagcaca ggttagctgt catttgaaat agcaggctgc
     6901 tagggctagc ggcgttatat ctggctaaaa aatccccatg cagcttcgat aaactgtggt
     6961 tttttaatca aatttgcctt gtcagctcgc catcgccgtg tatttcaact gccggttaga
     7021 agtgtgcgcg caaacttagg tacactggcg cgtttttttg aggccgcatc agtgcggctg
     7081 cctgttgact agccgtatga gatgcttatg aacaacctaa aatcactggt cctgtggggg
     7141 gcggttgccc tcttaggggc ttgggccttt gcggtattgg ccctgaaaca gggcgaaacc
     7201 gtgaatgcgc tctggattgt ggtcgctgct gtgtgcgttt atctcattgc ctatcgcttt
     7261 tatgcacaat ttataggcaa aaaagtaatg caattagacg ataaccggcc cactccggca
     7321 accattcgca acgatggact ggactatgtg ccaacccaca aatgggtgtt atttggccat
     7381 cactttgcgg ccattgccgg cgcagggcca ctggttgggc ctattcttgc ggcgcagatg
     7441 ggttatcttc ccggcacttt gtggatatta gtgggtgttg tattgggcgg ggctgtgcag
     7501 gatgctctgg tgttatttgc ttccagccgc cgcgatgggc gctcattagg tgaaatgatt
     7561 cgccaggaaa tggggcctgc cgcaggcgca atcggtatgt tgggcattct ggccatcgtt
     7621 atcattcttt tggcagtgtt gggcatggtg gtggtgaaag cactagccca cagtccttgg
     7681 ggattattta ccatcgccgc aaccattccc atcgctttat ttatgggcat ttattcgcgc
     7741 tattggcggc ccggcaaagt cggtgaggta tcgtttatcg gttttatttt attgatggcg
     7801 gcacttattg gcggtgaatg ggtgagccat cagccttttg ctcaggagta cctatcgctg
     7861 tcggcagagg cgctggcgct ggggttaatg gggtatggat ttatcgctgc ggtgctgccg
     7921 gtctggttgt tgttggcgcc gcgggattac ctttccacct ttttaaaaat tggcaccata
     7981 gtgggtttgg cgctgggcat tgttattacc gcgccggaat taaaaatgcc tgcaattacc
     8041 caatttattg acggcagcgg tccggtgttt gccggttctt tgttcccgtt tttatttatc
     8101 accatcgcct gcggcgcaat ttcgggcttt cactctttag tggcttcggg cacaacaccg
     8161 aaaatgctcg c
//
LOCUS       DAOQGS010000129         8161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4181_length_8161_cov_4.797927, whole genome shotgun sequence.
ACCESSION   DAOQGS010000129 DAOQGS010000000
VERSION     DAOQGS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_8161_cov_4.797927"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKZ52_06590"
     CDS             <1..678
                     /locus_tag="PKZ52_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNG59475.1"
                     /translation="PSAILLFLLQPWSDFLSFVMLRHNEESEEWEKALSLVDELLWII
                     EPKLQADDVEQQLQMLAPMLSYIREGFNTIGYDHDKGEKLLEAINSLVELAQQNKKAE
                     PAPAPMRDQLEKIAAEKAGPKPQVFVDLSEEEAKVVDNLKLIEFGTWFEFEGGKRLKV
                     AWYNARTSHYMLVDQMGKKVAMKSGLELARDMISNQAKIISGSSKPFFERALENIRVK
                     LAEQQAQ"
     gene            699..1025
                     /locus_tag="PKZ52_06595"
     CDS             699..1025
                     /locus_tag="PKZ52_06595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG59476.1"
                     /translation="MGQDKRTRQRLTVNNDVFVRDRSDGELLGKVANIHLEGFMLIGS
                     RTLREDALFPVLLEADGLSIAANAECLWTSETGSGDQSWAGFGFLDLDPEEERKLAEL
                     VQRFEV"
     gene            1188..1619
                     /locus_tag="PKZ52_06600"
     CDS             1188..1619
                     /locus_tag="PKZ52_06600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNG59477.1"
                     /translation="MIKTVFLSALALLICACAPLQPKESIERKHYESGEADIGYSQVL
                     RVGKVLYISGVVSTAPTLQQQLDEEYTFIRKILGDYGASTDAIVKETIYTLDMAALKQ
                     AIPARKKYFPNNLYPSASWVQVKALYDPAILLEIEVEAHLP"
     gene            2358..3092
                     /gene="fnr"
                     /locus_tag="PKZ52_06605"
     CDS             2358..3092
                     /gene="fnr"
                     /locus_tag="PKZ52_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNG59478.1"
                     /translation="MSLLPKTCHLHNQTHCGDCRLHALCLPLSLQIEDVGKLDDIVQR
                     GRPLQKGDVVYRAGELFHAIYAVRSGAVKTLSHSAEGDEQVTGFYLPGEIIGLDGIAT
                     GQHTNTAIALETSAICEIPFNRLEELSIQLPNLQRRFLQIMSRELASDQQLITLLSKK
                     AAEERIAAFLVSISRRNQGRGLVGNEFYLPMSRSDIGNFLGLTIETVSRVFSRLHKAG
                     IIQLDKKHVVIADMAALLDATAAEKF"
     gene            3105..3707
                     /locus_tag="PKZ52_06610"
     CDS             3105..3707
                     /locus_tag="PKZ52_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HNG59479.1"
                     /translation="MMTEALFSRCIALFDDANRQDPNTELCNGEPVPKELLYAQRMSK
                     ALLAFAPNASFALQLAARAQHIERWTSPRSNYPEGRTGYKQWRAELLLYHARRAGELM
                     AQAGADGDEIARVQYLIQKRSMARDPETQCLEDVICLVFIEHYLADFAVKSGYDEAKL
                     IDIIQKTWVKMSPRGQAAALQLPLAAEVAAVIGKALAGAD"
     gene            complement(3746..4756)
                     /locus_tag="PKZ52_06615"
     CDS             complement(3746..4756)
                     /locus_tag="PKZ52_06615"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HNG59480.1"
                     /translation="MSLILEALNKADRERDNQPKVPDLQTVHGRIVPPSPPKRHWGVY
                     VIIALLVLILFLLIAVLVRQNMAPTTKAPASPPTQAAPAAAPATVAQPVAAAPAPAAM
                     APAPPTTLDTGSDVHDIYQDATMSAPPPEYSSGYEEAPALPRSKKSRSRLLDELEAQA
                     QELDEANNGSEEDLVPASVRKSSKPVTKTADSTQENGELSEEKIKELWEQTKKEIPDR
                     PANQAKLQTQIQEAIKDTLSLYKQVPYLHELPEKVQNSVPSINYVNHLYSEKAGGVVI
                     INKKTLRNGQEIGPGMVVERVASDGIVVQYQGLRFKLNALSSWVNFSNKNPAPASEGK
                     KK"
     gene            complement(4760..6442)
                     /locus_tag="PKZ52_06620"
     CDS             complement(4760..6442)
                     /locus_tag="PKZ52_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG59481.1"
                     /translation="MYHSYFGIEEQAFSIAVNPRYLYMSAQHREALAHLLYGVNSGGF
                     VMLTGEVGTGKTTIIRCLLEQLPKHADVAMIMNPAASARDLLCSICDELGIDYLPDET
                     SLKILTDKLYEFLLTNHSKGRNTIVLIDEAQLLRVSTLEQIRLLTNLESNTQKLLQII
                     LVGQPELNELLAKPALRQLSQRITARYHLRPLSQDETGAYIKHRLQIAGMPPGRQPFP
                     EPIIKKVHAISGGIPRLINILCDRMLLGAYTQETTQIDHNIYKQATLEVMGERSRPTA
                     PNYQTYFYLAAIALGLMVAALLIWLLLAPQHKTDAAPPAAASAPAQETTAPPTTAATS
                     TPATTPPTATAPPTAFTSPPVAEGELFWQPTQNQALADLLGYIGVEKSNAMYPCWVVN
                     KEGLHCETQKAATWDDFKRFNRPAVLSLTTKTRQAGYVALIGIDGLEGELLVNGQRKR
                     ISLETLGGLWTGEFVFIWRKPEGFEGPAGLGSKAPIVAWVAQRFASIDKQTAPLTTGV
                     FNPPLQRRIKMFQQAHGITDDGLITLPTLLKLNDSLKIDKTLLTPAAAAAPN"
     gene            complement(6642..6836)
                     /locus_tag="PKZ52_06625"
     CDS             complement(6642..6836)
                     /locus_tag="PKZ52_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59482.1"
                     /translation="MGESLSTNSLVRRRLDPAAVRRLEREIATLRQHLSRMKSRPELA
                     DAKTLRTYEEMIEQRLQLLP"
     gene            complement(7014..7544)
                     /gene="cobU"
                     /locus_tag="PKZ52_06630"
     CDS             complement(7014..7544)
                     /gene="cobU"
                     /locus_tag="PKZ52_06630"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNG59483.1"
                     /translation="MPTCLYLGGARSGKSRLAQTRAEAHSAVTVIVTAEALDGEMAQR
                     IRHHQTQRPQHWQVLEAPLQLGAALLSCQARNEPPLVLVDCLTLWLSNLLCLEDDKRY
                     QLERTNFLQAVTKQRSPLLLVSNEVGLGLIAPTPLGRLFVDEAGRLHQELAGLCDEVI
                     WVAAGLPLWLKPCRGE"
     gene            7654..>8161
                     /locus_tag="PKZ52_06635"
     CDS             7654..>8161
                     /locus_tag="PKZ52_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNG59484.1"
                     /translation="MPIKAPLLFLLLILALLLAMTWALGAGSIALNWGDIWRGLWQPE
                     SSEGLLIWQLRIPRLLAAACCGALLALAGALMQLLLRNPLADPAIIGVSAGASFVALT
                     ALGLGFSAAGLPFYSFAGALLAIALVLLLADVRRGWQVQRVLLTGVVVAAGFGAGISF
                     WLTLSSDLR"
BASE COUNT         1670 a         2001 c         2423 g         2067 t
ORIGIN      
        1 ccttcggcga ttttgttgtt tttattgcaa ccctggtcgg attttttatc ctttgtgatg
       61 ctgcgtcaca acgaagaatc ggaagagtgg gaaaaggctc taagtttggt tgatgagctg
      121 ctgtggatta tcgagccgaa attgcaagcc gatgacgttg aacagcaact gcaaatgctg
      181 gcgcctatgc tcagttacat tcgcgagggt tttaacacta tcggttacga tcacgataaa
      241 ggtgaaaaac tgcttgaagc cattaactct ttagttgaat tggcacagca aaataaaaag
      301 gcggagcctg cgcctgcacc tatgcgcgat cagctggaaa aaattgccgc tgaaaaagcc
      361 ggcccaaaac cacaggtgtt tgtggattta tccgaagaag aagccaaggt ggtcgataat
      421 ttaaaattaa ttgaatttgg cacctggttt gaatttgaag gtggcaaacg tttaaaagtt
      481 gcctggtata acgcgcgcac ctcgcactat atgttggtgg atcaaatggg taaaaaggtc
      541 gccatgaaat cgggcctaga attggcccgc gatatgattt ctaaccaggc taaaattatt
      601 tctggcagct ctaaaccctt ctttgagcgc gccttagaaa atattcgcgt taagttagcg
      661 gaacaacaag ctcaataacc aactagcggg ggttttaaat ggggcaggac aagcgcacac
      721 gccagaggct taccgtcaac aatgatgttt ttgttcgcga tcgttccgat ggtgagttat
      781 tgggtaaggt cgccaatatt cacttagaag gttttatgtt gatcggctcg cgcaccttgc
      841 gggaagacgc tttatttcct gtcttattag aggccgatgg cctcagcatt gccgccaatg
      901 ccgagtgttt gtggacctcc gaaactggat caggcgatca gagttgggct gggtttggct
      961 ttttggattt ggatccagag gaagagcgca aattggctga gttggtgcag cggtttgagg
     1021 tgtagccttg cgaggcaggt ctttgtgtgc agcctttttc gctgtttgtt aagcgtataa
     1081 tatttctact tgcccatatc taaattaagc ggtgataggc cgcttatttt ttaaaacttc
     1141 aatccattgt ttaaagccgt tttaaacatc gagtgagcac aatgcctgtg attaaaactg
     1201 tctttttatc cgcgttggcg ctgttgattt gtgcctgtgc tccgctgcag ccaaaagaat
     1261 cgattgagcg caagcactac gagagcggtg aagcggatat aggttattct caggtgctgc
     1321 gggtggggaa ggtcctgtat atttccggtg tggtgagtac tgcgccaacc ctgcaacagc
     1381 agttggatga ggaatatact tttatccgta aaatcctcgg tgattacggc gcgagcaccg
     1441 atgcgattgt gaaagaaact atttatacct tggatatggc ggcgcttaag caggccattc
     1501 cggcgcgcaa aaagtatttc cccaataacc tctatccgag tgcatcttgg gtgcaggtga
     1561 aagctctgta cgacccagcg attctgttgg aaattgaggt ggaagcacat ttgccctagc
     1621 tgctcgctga aactcctagc aatctagcgg cttgctgaaa taccaccttg cgagcctggg
     1681 cgtcgaagcc tgggtatgtc tacacgctgc tagtgtttca gcgagccgag catcggttga
     1741 atttttctgt aattacaatc gtcgccagcg ggaagctcgc cgcctaacag caaggcgaac
     1801 ctctgtgttt ttacactatt cacgagtaac tattcagcat ctttttatgt ggctgaaggt
     1861 aattcctgca ctgacaggga actatcgcgc gcgcgtttta gtgccgtgac cccgtaatcc
     1921 agcgcccgca gcagcgacta caggttagtg cagacacgcc gtgaatacat cctaagtagg
     1981 ctcgtcaaaa aggtccctct ttttgacggt ctgcactaac ctgtagtcac cactgctaca
     2041 ccgcgaatcg attatggcgg atggctaaat cctcctgaat ttgcctcccg cgcggaagag
     2101 gggagtaaag tgtacattca cgatttcagt tttatcagag cttctatcaa cttgaggtac
     2161 gcccaacacg cgcccgaaat aggcacgatt actgctgaag ggcagtttta ggtagcgcga
     2221 cagaaactgc tttatggcct gtataaatgt tatattgcac gcttttattg cgccgcaggc
     2281 ttgtccgtca taagggtttt gcaaaaggct tgtggcgcat ttccattttt caaagacttt
     2341 tataccggca cttttttatg tctttacttc ccaagacctg ccatttgcac aatcaaaccc
     2401 attgtggtga ttgtcgcctt catgccctgt gtttaccgct gagcttacaa attgaagatg
     2461 ttggcaaact agatgatata gtgcagagag gacgaccgct gcaaaagggt gatgtggtgt
     2521 accgggccgg ggagctgttc catgcgattt atgctgtccg ctccggcgcg gtgaaaaccc
     2581 tcagccacag cgccgaaggc gacgagcagg tgacggggtt ttatttgccc ggtgaaatta
     2641 tcggcctgga cggcatagca acagggcaac ataccaatac cgccattgcc ttggaaacca
     2701 gcgcaatttg tgagattccc ttcaatcgct tagaagagct gagcattcaa ttgccgaatt
     2761 tacagcggcg ttttttgcag attatgagcc gcgaactcgc cagtgatcag cagttaatta
     2821 ccctattgag taaaaaggca gccgaggagc gcattgcggc atttttggtg agtatctctc
     2881 ggcgcaacca ggggcgcggc ttggtgggta atgagtttta tctgcctatg tcccgctccg
     2941 acattggcaa ctttttaggt ttaactattg aaaccgtcag ccgtgtgttt agccgtttac
     3001 acaaagcggg cattatccag ttggataaaa aacacgtggt tatcgccgat atggcggccc
     3061 tcctggatgc cactgcggct gaaaaattct aatcggtgac cgctatgatg actgaagcct
     3121 tattttcccg ctgtatagcg ctatttgatg atgccaatcg acaggacccc aatactgagc
     3181 tgtgcaacgg cgagcctgtg ccaaaggaat tgttgtacgc ccagcgcatg agcaaggccc
     3241 tgttagcgtt tgcgccgaat gcctcctttg cgctgcaact tgccgcccgc gcccagcata
     3301 tagagcgatg gaccagcccc cgcagcaatt accccgaagg gcgcaccggt tataagcaat
     3361 ggcgagcgga gttgctcttg taccacgccc gccgcgccgg ggaattgatg gcgcaagcgg
     3421 gcgccgatgg ggatgagata gcccgcgtgc agtatttaat tcaaaagcgc tctatggccc
     3481 gcgacccgga aacccagtgc ctggaggatg tcatttgcct ggtatttatc gagcattatt
     3541 tggcggattt tgcggttaag tccggctacg acgaggctaa actcatcgat attatccaaa
     3601 aaacctgggt aaaaatgtcg cctcgtggcc aggcagcggc actgcaattg cccttggcag
     3661 cagaggtggc ggcggtaatt ggcaaggccc tggcgggagc agattgatgg gttgaagatt
     3721 cggcgccact ggtagtcaat agccgttatt tcttctttcc ctcgctcgcc ggagcagggt
     3781 ttttgttgga aaagttcacc cagctgctca aggcgttgag cttaaagcgc agaccctggt
     3841 actgcaccac aatgccatcg gatgccaccc gctcaaccac cattcccggg cctatttcct
     3901 gtccattgcg cagggtcttt ttgttgataa tcaccacgcc gccggccttt tctgaataca
     3961 ggtggttgac gtaattaatt gacggcacac tgttttgtac tttctcgggc agctcgtgca
     4021 ggtagggcac ctgtttgtac agagagaggg tgtctttgat ggcctcttga atttgggttt
     4081 gcaacttcgc ctggttggcg gggcggtcgg ggatttcctt tttggtttgc tcccacaact
     4141 ctttaatttt ttcctcactc agctcgccgt tttcctgtgt gctgtcagcc gttttggtca
     4201 caggcttgct gcttttgcgc acgctggcgg gcaccaagtc ctcttcgctg ccgttgttgg
     4261 cttcatctaa ttcctgggcc tgcgcctcca gctcatccag caggcggctg cgggattttt
     4321 tgctgcgggg cagggcgggc gcctcttcat aacccgatga atattcgggc ggcggggcgc
     4381 tcatagtggc gtcttggtag atatcgtgca catcgctgcc cgtatccagc gttgttggcg
     4441 gggcgggtgc catcgcggca ggcgcggggg ctgcagcaac gggctgggca acagtggcag
     4501 gtgcggccgc tggcgccgct tgggttgggg ggcttgccgg ggccttggtc gtcggggcca
     4561 tattctggcg caccaatacc gcaatgagca gaaataaaat cagcactagc agcgcgatga
     4621 tgacatacac cccccagtgg cgtttgggcg gggagggcgg cacaatgcga ccgtgcaccg
     4681 tttgcagatc cggcacctta ggctggttat cgcgctcgcg atccgctttg ttgagggctt
     4741 caagtatcag ggacataaat taattgggtg ccgcggcggc tgcaggggtt aggagcgttt
     4801 tatcaatttt taacgagtca ttgagcttca acagtgtcgg cagggtgatt aagccgtcgt
     4861 cggtaatgcc atgggcctgt tggaacatct tgatgcgccg ttgcaagggt gggttaaaca
     4921 ccccggtggt taagggcgct gtttgtttgt cgatgctggc aaaacgctgg gccacccagg
     4981 ccacaatcgg cgccttggag ccaaggcccg ccgggccttc aaaaccctcc ggtttgcgcc
     5041 agataaacac aaattcaccg gtccacaggc cgcctagggt ttccaagctg atgcgtttgc
     5101 gctggccgtt gaccagtaat tcaccttcta agccatcaat gccgatcaat gccacataac
     5161 cggcctggcg ggttttggtg gtgaggctga gcaccgccgg gcggttaaaa cgtttgaaat
     5221 catcccaggt tgcggccttt tgggtttcgc agtgcaggcc ctctttgttc actacccagc
     5281 aggggtacat agcgttgctt ttttccactc caatatagcc gagtaaatcc gccagcgcct
     5341 gattctgggt gggttgccag aacagctcac cctcggccac tggcggggag gtaaaggctg
     5401 tgggcggggc tgttgcggtt gggggggtgg tcgcaggtgt gcttgtggcg gcggtcgttg
     5461 ggggcgctgt agtttcttgt gccggggcgc tggccgctgc cgggggggct gcgtcggttt
     5521 tgtgttgtgg ggcgagcaac agccaaatca gcagcgccgc taccatcaag cctaaagcga
     5581 tggcggccag gtaaaaatag gtttggtagt tgggtgcagt ggggcgcgag cgctcaccca
     5641 taacctcgag ggtggcttgt ttgtatatgt tgtggtcgat ctgcgtagtt tcttgggtgt
     5701 aggcacccag cagcatgcgg tcgcagagga tattaatgag cctgggaatg ccaccgctga
     5761 tggcgtggac ttttttaatg atcggctcgg gaaacggctg gcggcccggc ggcataccgg
     5821 cgatttgcaa gcggtgtttg atgtaggcac cggtttcgtc ctggcttaag gggcgcaggt
     5881 ggtagcgggc ggtgatgcgc tgggataact ggcgcagggc gggtttagcc agcagttcat
     5941 tgagctccgg ctggccgacc agaataattt gcagcagttt ttgggtgttg ctctccaggt
     6001 tggtgagcag gcggatctgc tccaaggtgg atacccgcag cagctgcgcc tcatcgatca
     6061 acactatggt gttgcggcct ttgctgtggt tggtgaggag gaattcgtag agtttatcgg
     6121 tgaggatttt taagctggtt tcatcgggca ggtaatcgat acccaattca tcgcagatac
     6181 tgcacagcag gtcccgggcg ctggccgccg ggttcataat catggccaca tcggcgtgtt
     6241 tgggcagttg ctccagcagg cagcggatga tggtggtttt gccggttccc acttcgccgg
     6301 tcagcatgac aaacccgccg ctgtttaccc cgtacaacag gtgcgctagg gcttccctgt
     6361 gttgggcgct catatagagg tagcgcgggt taacggcgat ggaaaacgcc tgttcttcaa
     6421 tgccgaaata actatggtac atagggtatg tttacgacct tgcttcttat tgtagtgtcg
     6481 ggaagttaac ccaggacgcc gcgtaagcca aggctgggac tttaagccct acaagcgaca
     6541 agtggagtat aagcgccgcc gtgtcacaag cgcaaaaaat gccgtgtttt taaggggcaa
     6601 aaagggtgtt aggggtgcgg ggccgcaata ggggggaaga cttaaggcag cagttgcagg
     6661 cgttgctcaa tcatctcttc gtaggtgcgc agggttttgg cgtcggccaa ttccgggcgg
     6721 cttttcatgc ggctgaggtg ttggcgcagg gtggcgatct cccgttcgag tcggcgtaca
     6781 gctgccgggt ccaggcggcg gcgcacaagt gagttggtag agagggattc gcccattttt
     6841 aatgccatta accgattcgt gtgatttatt ttggtgctta agtatgagtt actttagcgg
     6901 aaattgcatg cttcggtaag aggggcgtgt gaattttttg aagcaataaa cgccattcgt
     6961 ccggttgttg ctcgccagcc catcattacc tagatctgca gttccttggg ggcttactcg
     7021 cccctgcaag gtttcagcca aagtggcaaa cctgcggcta cccagatcac ttcatcacac
     7081 aggccggcaa gttcttggtg caagcggccc gcttcatcca caaaaagtct acccaatgga
     7141 gtgggggcaa tgaggcccag gcccacttca ttgctcacca gtaaaagcgg cgagcgttgt
     7201 ttagtcacgg cctgcaagaa attagttcgc tccaattgat accgtttgtc gtcttctaaa
     7261 cacagcaggt tcgacagcca cagggtcagg caatccacca gcaccaaggg cggctcgttg
     7321 cgcgcttggc agctcagcag cgcagcgccc agctgcagcg gggcttccaa tacctgccag
     7381 tgctgggggc gttgggtttg atggtgtcgg atgcgctggg ccatttcgcc atcgagggcc
     7441 tcggcggtga caatcaccgt gacggcggaa tgggcttcgg cgcgggtttg tgccaggcgg
     7501 cttttgccac tgcgggcgcc gccgaggtag agacaggtgg gcatgctgga gcctttcaag
     7561 cgagtgagtg ggggcgggat actcccacac tcagccgcag caggtgaacc tgtatcgctt
     7621 gggattatac tggcgccttt ttgagccgag cctatgccca tcaaggcgcc tttgttattt
     7681 ctacttttga tcctcgccct gctgttagct atgacttggg ccttgggggc gggcagtatt
     7741 gccctcaatt ggggggatat ttggcgcggg ctatggcagc cggagtcttc tgagggcctg
     7801 ctgatttggc agctgcgcat tccccgcctg ctggcggcgg cctgttgcgg tgcgctgttg
     7861 gcactggcag gtgctctgat gcagttattg ctgcgcaacc ccctggcgga cccggcgatt
     7921 attggcgtct cagcgggcgc ctcgtttgtt gccctcacgg cgctgggctt gggttttagc
     7981 gcagcgggct tgccgttcta cagttttgcg ggggcactgc tggcgattgc gctggtgctg
     8041 ctgctggcgg atgtgcggcg gggctggcag gtgcagcggg tgctattgac cggcgtggtg
     8101 gtggccgcag gcttcggggc gggcattagt ttttggctga ccctcagttc tgatctgcgc
     8161 c
//
LOCUS       DAOQGS010000130         8130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4203_length_8130_cov_5.021424, whole genome shotgun sequence.
ACCESSION   DAOQGS010000130 DAOQGS010000000
VERSION     DAOQGS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_8130_cov_5.021424"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKZ52_06640"
     CDS             <1..322
                     /locus_tag="PKZ52_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNG59485.1"
                     /translation="RHSGDVTAEVADQALNLLNVDACGFDHMDRRLLLTLIEKFSGGP
                     VGVDSLAAAISEERDTIEDVLEPYLIQQGFLIRTPRGRQATALAYAHFDCVMPKRLRQ
                     DELF"
     gene            480..1169
                     /gene="tolQ"
                     /locus_tag="PKZ52_06645"
     CDS             480..1169
                     /gene="tolQ"
                     /locus_tag="PKZ52_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNG59486.1"
                     /translation="MPEQEPLSILHLIFGAGPVVLAVMGILFSASVISWIVIFQRAIY
                     QGKASRDFAEFERQFWSGIDLTQLYRQGNNKAADGTAEGIESIFRSGFKEFTRLRQQS
                     GVDADAVMEGAQRAMRVAAAKEQEKLEAGLPFLASVASVSPYIGLFGTVWGILNSFRG
                     LATQGQATIATVAPGISEALVATAIGLFAAIPAVLAYNRFAAKAETISSNYTTFAEEL
                     SSILHRQAHAR"
     gene            1185..1628
                     /gene="tolR"
                     /locus_tag="PKZ52_06650"
     CDS             1185..1628
                     /gene="tolR"
                     /locus_tag="PKZ52_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNG59487.1"
                     /translation="MAAVVKGGKRKKPMAEINVVPYIDVMLVLLVIFMITAPMLTQGV
                     KVELPKASAKPVDAKKDVINVEVKKDGSYWLDVGKESAEAGGTQMPLADIKQKVAILL
                     NADPATPVLVRGDKAVEYGVVVTLMAELQQAGAKSVGLVTDPNSQ"
     gene            1632..2543
                     /gene="tolA"
                     /locus_tag="PKZ52_06655"
     CDS             1632..2543
                     /gene="tolA"
                     /locus_tag="PKZ52_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNG59488.1"
                     /translation="MSEAVRKNAIPTLASLTLHLGLLGGLYYWETHRDPPPKVVEVQV
                     IEAKLVALKTKTKVAHQEDTRKVNKVEVSQAVEKEQDIKNRQEDDRKAAAEKLAEETQ
                     KKAQQEAQRKEQEAKVEEELALKRKKEEEKEKEKEAKEKAEAEAKAAEEKRKAEELAA
                     KAQEEKRKSLEDKIRKEQQAEKKKQELLKLKQAQERQVRAEQAEEAANSYLAMIKDRI
                     ERKWNRPPSARNGMACILHISIVPTGRIVDVEVVKSSGSEEFDRSAEQAVRAVEQFKE
                     LQGMDPDLFEQDFRELEILFRPEDLRQ"
     gene            2540..3817
                     /gene="tolB"
                     /locus_tag="PKZ52_06660"
     CDS             2540..3817
                     /gene="tolB"
                     /locus_tag="PKZ52_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNG59489.1"
                     /translation="MRFVIALLLSWITLSAHALLTVEITKGSAKPTPIAVVPFGNTNG
                     LPEDIQQIVSADLDRSGLFQTIPKGEMLSQPTTQKEVYFRDWQRLKVSYVVIGSMSMS
                     PDGRYALHFELYDVLSSRQVFSGNTVGQVSQLRDIAHAVSDAVFEKITGIPGIFSTKM
                     VYVEDLGYAGPGRYRLVFADIDGARERVLQASKMPILSPVWSHRGDKIAYVSFETTRP
                     AIYIMDLATGRKTQMTNFPGLNGAPAFSPDDTRLAMCLSKDGNPEIYVMNIATRQLQR
                     ITDHFGIDHEPSFSADGRSVLFTSSRGGSPQIYQVDLASGRPERVTFDGEYNARPRVT
                     PDGKSIVMVHRDKGIFHIASQDLKTGDMRILTETLLDESPSVAPNGAMLLYATQQQGK
                     GVLGAVSMDAGVKFTMPSRQGDVREPAWSPFLK"
     gene            3864..4349
                     /gene="pal"
                     /locus_tag="PKZ52_06665"
     CDS             3864..4349
                     /gene="pal"
                     /locus_tag="PKZ52_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNG59490.1"
                     /translation="MLNTFKTVFALIAVLGLLSGCSSSKKKQESTAPAATTAEAPAAA
                     GVGIDALKAAADAAGNVFYFEFDSFVLTEQTRAALLAHAEYLKAAPRSIRLEGHADER
                     GSREYNMALGEKRAAAVKDFLASQGVSSAVEVVSFGEEQPAEKGHSEASWSKNRRVEL
                     K"
     gene            4363..5313
                     /locus_tag="PKZ52_06670"
     CDS             4363..5313
                     /locus_tag="PKZ52_06670"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG59491.1"
                     /translation="MLKSSLAAIFVMAVSSSAIAKVEVIDSEPVSSPGAVTVYPGPAP
                     SVTPSVSSRVASGSSDYSQIQAMQEEIQQLRGLVEQQANDIKRLKTQREEDYMDLDRR
                     ISALGKGGASTASHPADEAGLSEKKPSLTEKKSPKKDPASSNQSLSSEAPEEALVDAA
                     VATSSVAAVPNKKPRIADDPSENADGSSSADAGADEFTVYSSALNLIVKSKDYDGGIA
                     AMNKYLAQFPKGKYAPNALFWVGSAYQSQNKPDKAIESYERMIKRYPKEVKSDEARLR
                     LARVYFQRGDKEEARVVLDEIVEGGGPQAKAAQDMLSKNF"
     gene            5412..6692
                     /locus_tag="PKZ52_06675"
     CDS             5412..6692
                     /locus_tag="PKZ52_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HNG59492.1"
                     /translation="MSSNSGSPLVPERPLVVSVELAKQLGLEEAALVTILADAVQFLI
                     PQQRLGSPWYLLEEPLVAKLMPFWQPVDVQRLLANLKAKGVLALASAPYNQSRQINFS
                     FHARAAAHEVSVRGEYVPKPAATHNFIAPGWQPDNDTLSRIHQLNIPEDFALSQVGEF
                     VLYWRERNEPARAWGSKFLKHVLARWREQEAKQAIKQRQKPIHFDWRPSQEVIHALVN
                     SAGIEAKKIEELIPKFVHYWLMHGGAPENWNLEFHSYAMRNTARSQLPIFKGWRPSED
                     TLEVMVQAGISREFIEDAIPEFELYWREQKAESDNWNRKFRDHVQRQWLSYQSALEHD
                     TVPRRIPSNWQPSEDVYDVLRLANIDVTYAQELLPEFVIYWRDSNQVYHSWNTRFLQY
                     VKKRWASAEKLETAQRSTREISLVDELTDRSWAQ"
     gene            6655..7284
                     /locus_tag="PKZ52_06680"
     CDS             6655..7284
                     /locus_tag="PKZ52_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HNG59493.1"
                     /translation="MSTNSPTAAGPNNSSRTSGAKATPELIDAINQVFALFRRNYLNQ
                     YHKAFGDVEELNHTKRLWLEILSRFTPQAILMGARGVIENSEYLPSLHTMIRYCEQAD
                     SSTAFPDPHSAYSEACRAPSPKAVYKWSHLAVYYAGKACDWYFLQTQSEAVAFPVFRE
                     KYLELCAKVRAGLALPEPTPPAAAAIEAVPTDKGQAIEKLAALRAKLEL"
     gene            complement(7299..7922)
                     /locus_tag="PKZ52_06685"
     CDS             complement(7299..7922)
                     /locus_tag="PKZ52_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59494.1"
                     /translation="MSELKFLGRATISLTLFLFCSCGSFTNKPAYKLAAPPEMDITFT
                     GRGTASGPMLMSAMGPAGIAVGMAIDVGIGKDIEKFGFADGLKVDELFAESITQVAQN
                     QKFNRGFFSSHYSSNFQLLKIGFIEPPGQNDCIQPTLVINVLKGGWHKTYQYPEDFQA
                     DKNMPLPGASLEQLKSPQSPARSLLKQAFTQVIEKCAVDWQAESTKK"
BASE COUNT         2152 a         1924 c         2087 g         1967 t
ORIGIN      
        1 gcgccactcc ggcgatgtta ccgccgaagt ggccgaccag gccctgaatc tcctaaatgt
       61 agatgcctgt ggctttgatc acatggaccg acgcctcctg ctcaccctga ttgaaaaatt
      121 ctccggcggc ccggtagggg tcgattcact tgctgcggcc atcagtgaag agcgcgacac
      181 catagaagac gtactcgagc catacctcat tcagcagggc tttttgatac gcacgcccag
      241 aggccgccaa gccaccgccc tagcctatgc ccattttgat tgcgtgatgc ctaaacgcct
      301 gcgccaagat gagctgtttt agtgcctctc caacaatcta cctcctgagg ccagttgttt
      361 ttgtggacga agtccgcaaa acatagtgct tggcaaaaca tagtgctatc atgccgccgc
      421 ttttttgggg cccccgctat agcgggcctt gagtgatgtt aacttggaat tcccgccgca
      481 tgcccgaaca agaaccctta tccattctgc atctcatttt tggtgcaggc cctgttgtat
      541 tggccgtcat gggtatttta tttagtgcat ccgtgatttc ttggatcgtg atcttccagc
      601 gcgccatata ccaaggtaag gcgtcccggg attttgctga attcgagcgg cagttttggt
      661 ccggcattga tctcacccag ctctaccgcc agggcaacaa caaagcggct gacggcaccg
      721 ccgagggcat cgaaagtatt tttcgctcag gctttaaaga atttacccgt cttcgccagc
      781 agtccggtgt cgatgccgac gccgttatgg agggcgccca gcgcgctatg cgcgttgccg
      841 ccgcaaagga gcaagaaaaa cttgaggcgg gtctaccctt cctggctagc gtggcctcag
      901 taagtcccta catcggcctc ttcggcacgg tttggggtat tctgaattcc ttccgcgggc
      961 ttgccaccca ggggcaagcc accatagcca cggttgcgcc gggtatctca gaggccctgg
     1021 tggccaccgc catcggccta ttcgccgcta ttcctgccgt gcttgcgtac aaccgttttg
     1081 ccgccaaagc agaaaccatc agctcaaact acaccacctt cgcggaagaa ttatcgtcca
     1141 ttcttcaccg tcaggctcat gcccgctaag gggcttaaat cgctatggca gcagtagtta
     1201 aaggcggcaa acgcaaaaag ccaatggctg aaatcaatgt ggtgccctac atcgacgtaa
     1261 tgctcgtgtt attggtcatt ttcatgatca cggcccctat gttgacccag ggcgttaaag
     1321 tggaattgcc taaagcttcc gctaaacccg tcgatgccaa aaaagatgtc atcaatgtgg
     1381 aggttaaaaa agacggctcc tactggctgg atgttggcaa agaatctgct gaagcgggtg
     1441 gaacccaaat gcctttagct gacattaagc aaaaggttgc tattttgttg aacgcagacc
     1501 cggccacacc tgttttggtg aggggggata aagcagtcga atacggtgtg gtagttacct
     1561 taatggctga attacagcaa gcgggggcga aatcagtggg cttagttacg gatcccaata
     1621 gtcaataagc tatgtccgaa gctgtgcgca agaatgcaat tccaaccctc gcaagcttaa
     1681 cgctacacct tgggttgctg ggtgggcttt attattggga aacccaccgc gatccaccgc
     1741 cgaaggtagt ggaagtgcag gtgatagaag caaaattggt ggctttaaaa accaaaacca
     1801 aagtcgccca tcaagaagat acgcgcaaag ttaacaaggt tgaggtatcg caggcggtag
     1861 agaaagaaca ggatataaaa aaccgtcagg aagatgatcg caaagcggca gcggaaaaac
     1921 tcgctgagga gacgcaaaaa aaagcccagc aggaagctca gcgcaaagaa caagaagcca
     1981 aggtagaaga agagttagcg cttaagcgca aaaaagaaga ggaaaaagaa aaggaaaaag
     2041 aagctaaaga aaaagcggag gcggaggcaa aagcggcgga agaaaaacgc aaggctgaag
     2101 aattagccgc caaggcgcaa gaggaaaagc gtaagtcgtt ggaagataaa attcgcaaag
     2161 aacagcaggc tgaaaaaaag aaacaggaac tattaaaact taagcaggcc caagagcgcc
     2221 aagttcgcgc cgagcaggct gaagaagccg ccaactctta cttggcgatg attaaggatc
     2281 gcattgagcg caaatggaat cgcccgccct ctgcccgcaa cggcatggcg tgtattttac
     2341 atatctcgat tgtgcccact gggcggattg tggatgtaga agtagtcaaa agcagtggca
     2401 gtgaagaatt tgaccgctcg gcagaacaag ctgtgcgtgc agtagaacaa tttaaagaat
     2461 tgcaaggtat ggatcctgat ctgtttgagc aggatttccg tgaattggaa atattattta
     2521 gacccgagga tttaaggcag tgagatttgt tattgctctg ttattaagct ggataacgct
     2581 gtctgcccac gcgttattaa cagttgaaat taccaagggt agcgccaaac ctacaccgat
     2641 tgccgtggtg ccctttggta ataccaatgg cttacctgaa gatattcagc aaattgtatc
     2701 agccgatttg gatcgcagcg gtttatttca aacaattccc aaaggggaaa tgctctcgca
     2761 gcccaccacg caaaaagaag tctattttcg cgattggcag cgtttaaaag tatcctatgt
     2821 tgtaattggc tccatgagca tgtcgcccga tggtcgctat gcgctgcatt ttgaacttta
     2881 tgacgtgttg tccagtcggc aggtttttag cggcaatact gtaggccagg tgagtcaatt
     2941 acgggatatt gcccacgcgg tgagcgacgc agtatttgag aaaatcactg ggatcccagg
     3001 tattttctcc accaaaatgg tatacgtgga agatctagga tatgcaggcc ctggtcgcta
     3061 cagattagta tttgccgata tagacggcgc ccgtgaacgt gtgttgcagg cgtctaaaat
     3121 gccaatactt tcacctgttt ggtcccatcg tggtgataaa attgcatatg tttcatttga
     3181 aactacgcgt ccagccattt atattatgga ccttgcgacg gggcgcaaaa ctcagatgac
     3241 caatttccct gggttaaatg gtgcaccagc gttttcgccg gatgacacac gtttagctat
     3301 gtgtttgtct aaggatggca acccagaaat ttacgtaatg aatattgcaa ctcgacagtt
     3361 gcaaaggatt acagatcatt ttggcatcga ccatgagccg agcttttcag ctgatggccg
     3421 ttcggtattg tttacatcga gtcgcggcgg gagtccgcag atttatcagg ttgatttggc
     3481 cagtggccgc cctgaacgag tgacatttga cggtgaatac aatgcgcgcc ctcgggtaac
     3541 acccgatggt aaaagtattg tgatggttca ccgtgataaa ggcatctttc atatcgcctc
     3601 tcaagatttg aagacgggtg atatgcggat attgacggaa actttgttag atgaatcgcc
     3661 aagcgtagct cccaacggcg ccatgttgtt gtatgctacg cagcagcaag gtaaaggagt
     3721 gctcggcgca gtctctatgg acgctggcgt gaaatttaca atgccgtcaa gacaaggtga
     3781 tgtacgtgaa cctgcgtggt caccattttt gaaatgatga gttaaattca actcgtaaaa
     3841 cctgttcact tgggagtgat aaaatgttaa acacttttaa aaccgtattt gccctgatcg
     3901 ctgtattggg tctgttaagc ggctgttctt caagcaagaa aaagcaagag tctactgctc
     3961 ctgctgctac cactgctgaa gctccagctg ctgcgggcgt tggtattgat gcgcttaaag
     4021 ctgctgcaga cgctgctggc aacgtgttct acttcgagtt cgactcattt gtgttgaccg
     4081 aacaaactcg cgctgctctg ttggctcacg ctgagtactt aaaagctgca ccacgctcta
     4141 ttcgtttaga aggtcacgct gacgagcgcg gcagccgcga gtacaacatg gctctcggtg
     4201 agaaacgtgc tgccgctgtt aaagacttct tggcttctca aggtgttagc tctgcagttg
     4261 aagttgtaag ctttggtgaa gagcaaccag ctgaaaaagg ccattcagaa gcttcttggt
     4321 ctaaaaaccg tcgcgtagaa ctgaagtaag tttttgacct gcatgttgaa atcctccctc
     4381 gctgccatct tcgtgatggc agttagcagt tccgccatcg ccaaggttga agtaatcgat
     4441 tcagaaccgg tttcttcgcc tggcgcggtt actgtttatc ctggcccagc tccgagcgta
     4501 acgccctccg tgtcgtcgcg cgtggcttcc ggctcgtctg attattctca aatccaagca
     4561 atgcaggaag aaattcagca gctgcgggga ttggttgagc aacaggccaa cgatatcaag
     4621 cgacttaaaa ctcagcgtga agaggactat atggacttgg atcgcaggat aagcgctttg
     4681 ggtaagggag gagcatcaac agcatctcat cctgccgatg aagcaggttt gagcgagaaa
     4741 aagccgtcgt taacggaaaa aaaaagcccc aaaaaagacc cagccagcag caaccaaagc
     4801 ctctcaagcg aagcccctga agaagcttta gttgacgcag cggtggcaac ctcttctgtg
     4861 gcagctgtac cgaataaaaa acctcgtatt gcagatgacc ccagcgaaaa tgctgacggg
     4921 agttcatcag ctgatgctgg cgccgatgag tttacggttt acagctcagc gctaaacctt
     4981 attgtaaagt ccaaagatta tgatggcggc attgcggcca tgaataagta tttggcgcaa
     5041 ttccccaagg gcaaatacgc acccaatgca cttttctggg ttggaagtgc ctaccagagc
     5101 cagaacaaac cagacaaagc tattgagtct tacgagcgca tgatcaagcg ctaccccaaa
     5161 gaagtgaaat ccgatgaggc gcgcttacgc ctcgcccgag tatatttcca gcgtggcgat
     5221 aaagaagaag cgcgtgtggt cttggacgaa attgtcgaag gtggcggccc tcaagccaag
     5281 gctgctcagg atatgctgag caaaaatttc tgacaggctc gcctcactaa acaatcaaaa
     5341 tgtacttgta gtctgcttgc ttgccgttta aggtctgcgc ggtcactttg tattaagtcg
     5401 gtagtatctg catgtcctcc aactccggta gccccctggt gcctgaaaga cccctcgtgg
     5461 tgtccgtgga gcttgctaaa cagttgggcc tagaagaggc tgcgttagta acgatccttg
     5521 ccgatgccgt acaattttta atcccgcagc agcgattagg cagtccttgg tatctactcg
     5581 aagagccgtt ggtagccaag ttgatgccgt tttggcagcc tgtcgatgtg cagcgattat
     5641 tggctaattt aaaggccaaa ggggttttgg cgctggcatc ggcaccctat aaccagtctc
     5701 ggcaaatcaa ttttagtttt cacgcgcgtg cagccgccca tgaggtttca gtgcggggag
     5761 aatatgtccc taaacctgcg gcaactcata attttatagc gccgggttgg cagccggata
     5821 acgataccct aagccgcatc catcaattga acattccaga agattttgcc ttgtctcagg
     5881 tgggtgagtt tgtactgtat tggcgtgaac gcaatgagcc tgcccgagcg tggggcagta
     5941 aatttctcaa acacgtcctt gcccgttggc gtgagcagga agccaaacag gcgataaaac
     6001 agcgtcaaaa accgattcat tttgattggc gccccagcca agaagtaatt catgcgcttg
     6061 tcaattctgc gggaattgaa gctaaaaaaa tcgaagaatt aattcctaag tttgtgcatt
     6121 attggctgat gcacggcggc gcaccagaaa actggaattt agaatttcac agttatgcca
     6181 tgcgcaacac ggcgcgcagc cagctgccta tttttaaagg ttggcggcca agtgaagata
     6241 cattagaggt gatggtgcag gcggggatta gccgtgagtt tattgaggat gcaatcccgg
     6301 aatttgaatt gtattggcga gagcaaaagg cagagtcgga taactggaat cgcaaatttc
     6361 gcgatcatgt acagcgccaa tggctgagtt atcaaagcgc cttggaacac gatacggtgc
     6421 cgcgccgcat ccccagcaac tggcaacctt cagaagatgt ttacgacgtt ttgcgtttag
     6481 cgaatatcga cgtaacctat gcgcaagaat tattgccgga atttgttatt tattggcgcg
     6541 acagcaacca ggtgtaccat tcctggaaca cgcggttttt acagtatgtt aagaagcgct
     6601 gggcgagtgc cgaaaaactt gaaactgcac aacgatcaac ccgggagata tcacttgtcg
     6661 acgaactcac cgaccgcagc tgggcccaat aattcttcgc gtacgagtgg ggcaaaagca
     6721 accccggaat taatcgacgc aattaatcag gtgtttgccc tatttcgtcg caattattta
     6781 aatcaatacc ataaagcatt tggtgatgtg gaggaattaa accacaccaa acggttatgg
     6841 ttagaaattc tcagccgctt tactccccaa gccatattaa tgggcgcccg gggggtaatt
     6901 gaaaatagcg aatatttacc gagcctgcat accatgatcc gctattgcga gcaggccgac
     6961 agcagcaccg cattcccgga tccccattca gcctacagcg aagcctgtcg agcaccctcg
     7021 cccaaagcgg tttataaatg gagtcatctc gcggtgtatt acgctggcaa agcctgcgat
     7081 tggtattttc tgcaaaccca atcggaggcg gtggcgtttc cagtgtttag agaaaaatat
     7141 ctggagttgt gcgcgaaggt acgagcgggg ttggctttgc ctgagccaac cccgccagct
     7201 gctgccgcaa ttgaggcggt accaacagat aaaggccaag caatcgaaaa actagccgcg
     7261 ttacgggcta aattagaact gtaaaacctg agtgaaatct atttctttgt cgattctgct
     7321 tgccagtcta cagcacattt ttcaatgacc tgtgtgaatg cttgcttcaa gagcgagcgg
     7381 gcaggggatt ggggggattt tagttgctca agcgacgcgc ctgggagcgg catattttta
     7441 tcagcttgga aatcctctgg gtattgataa gttttgtgcc agccaccctt tagcacatta
     7501 attactagtg ttggctgaat gcaatcattt tggccgggtg gctcaataaa cccaattttt
     7561 aataactgaa aatttgagct gtagtgtgac gaaaaaaaac cgcgattaaa tttctgattt
     7621 tgagccactt gcgtaattga ctctgcaaat aactcatcta cttttaatcc atcagcaaat
     7681 ccaaactttt caatatcctt gccgattccg acatcgattg ccatgccgac agctatcccc
     7741 gccggcccca tagcgctcat aagcatgggg ccggacgctg tgcctctgcc ggtgaatgtt
     7801 atgtccatct cagggggggc tgctaatttg taagcaggct tattggtaaa cgagccacat
     7861 gagcaaaaca aaaatagtgt gagtgaaata gtggctctgc ccaaaaattt taattccgac
     7921 atagacaaat aattgctcaa gaaatacttt taagtggtca agtgccgtat cgtgcggcga
     7981 cgatatggcc tacctaataa tggccactgg ccaagtggtt caaacagcgc cctaatccta
     8041 aaggagcgac gacgctaatt taaagtgttt tagcggcgat ttgttgtgtc caaaattgaa
     8101 atgtaatttt acggctgctc gactgaggcc
//
LOCUS       DAOQGS010000131         8098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4224_length_8098_cov_5.010320, whole genome shotgun sequence.
ACCESSION   DAOQGS010000131 DAOQGS010000000
VERSION     DAOQGS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4224_length_8098_cov_5.010320"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1408
                     /gene="nhaA"
                     /locus_tag="PKZ52_06690"
     CDS             227..1408
                     /gene="nhaA"
                     /locus_tag="PKZ52_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNG59495.1"
                     /translation="MGNWWHRITQSDAFSGILLMLAAVLALLAANSPLAGLYNQFLSL
                     KLVIAIGDFSIAKPLLLWINDGLMTLFFLMVGLELKREILVGELSQASKVAFPAAAAL
                     GGMLVPALIYTAFNYHDPIALNGWAIPTATDIAFALGVLSLLGSRVPAGLKLFLVTLA
                     IFDDIGAIVIIALFYTAKLSWLAISCAAIGLTLLAFMNWRGGINRTAYLLIGLFVWGS
                     LLKSGVHATLAGVAIALLIPYKGIDSSGREQTPVADIEHDLHSVVNLGVLPLFAFANA
                     GLSFVGLNPAALIAGVPLGICLGLVLGKQLGVMLFAWLAVALKIGRLPSDCNWSQIYG
                     AALLCGVGFTMSLFIGSLAFAEAAVSEPMHERLGILVGSVVSAVLGYFWLARVARPNS
                     E"
     gene            1472..2473
                     /locus_tag="PKZ52_06695"
     CDS             1472..2473
                     /locus_tag="PKZ52_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59496.1"
                     /translation="MEVVMAYQSLNSNSSTEHDVAASTRQAFQRAAQHTLAPMYPKGT
                     GVLRYPFNNDLDPSLILQGPAELVPVGDKNYLDDKKPSKKRKSDEADTVDDVQFAAAK
                     SEMLSMVEGKARQLYQFAYREVGATIDVIVFGELNSTHAQWQSINKEAGQKIHVSVRP
                     KACNCFSIHGPSGTTQSIAEGEGWCAVRCKGVLAVFVHVPNSLAANKDDATTFYKSIK
                     NILLNTPNGGVVDVVIGDTNQPRETFSPEVISSGMDQTFVDAHSGAILLSDTWAEKPV
                     IHKGTNSANSKKFDVAVYNTSTVKKIEVKYISQFSFAGRSAVAYTDHMGVLVKVDKK"
     gene            complement(2614..3270)
                     /locus_tag="PKZ52_06700"
     CDS             complement(2614..3270)
                     /locus_tag="PKZ52_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59497.1"
                     /translation="MKISVSLILGCALLSGCGTTNNMLTAKTKTVEYYRIFDIKTTAQ
                     LPDIISAASNGLGRNVNNADEATPIPDSNEIPDKPGRFKLVNPLAGSNLSAFAGGAGS
                     IGIKVATCDGARWTAKANRNTGGAGNLQLSACLYAYKGGYHLDLYAVFSKEEGGLTQV
                     ARSATYSLVGTPEQWTEKTFLDVVRMIKAKTKSDITLLEALPAISGTPWLDPLDAPKG
                     "
     gene            complement(3661..4389)
                     /locus_tag="PKZ52_06705"
     CDS             complement(3661..4389)
                     /locus_tag="PKZ52_06705"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNG59498.1"
                     /translation="MRIPRVYVALPLTAGVDISLPEAQAHYLTRVLRMEAGRPLIVFN
                     GEGGSHNAEIITAGKNNCVIRLVDFTADNHQSPLNCSLAIGISKGERFEFVLQKACEL
                     GVTQIYPLFTQRTEVRLDATRQSKKTEHWLQVLISSCEQNGRNLLPQLRAPQTLSEFL
                     PTVAADLKLVLHHRANQALNSYSTPHSVCLLIGPEGGLSEDEIKLAEQQGFNPLVLGP
                     RILRTETAPLVALSLVQYQWGDLR"
     gene            complement(4389..5228)
                     /gene="metF"
                     /locus_tag="PKZ52_06710"
     CDS             complement(4389..5228)
                     /gene="metF"
                     /locus_tag="PKZ52_06710"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNG59499.1"
                     /translation="MTDNIRLSFEFFPAKTDQGRDKLIQVQQELTAFKPEFFSVTYGA
                     GGSTREHTKGIVQLIKQAGQEVAPHLSFGGDSPEALLALLKDYQSHGINRLVALRGDR
                     PSGMGGGLHMVYANELVAFIRQHFGDYFHIEVAAYPEIHPESTSFISDIGFLKGKFDA
                     GANSAITQYFYNPDAYFYFVEQCRAQGIHQDIVPGIMPITNYKNLSRFSDSCGAEIPR
                     WLRKRLESFGDDNESLKAFGVDIVTELCETLLDNEAPGLHFYTMNQTDPTATILRNLG
                     WEN"
     gene            complement(5451..6821)
                     /gene="ahcY"
                     /locus_tag="PKZ52_06715"
     CDS             complement(5451..6821)
                     /gene="ahcY"
                     /locus_tag="PKZ52_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNG59500.1"
                     /translation="MSFTDYKVADMSLADWGRKEIEIAEGEMPALMALRRKYQASKPL
                     AGAKIIGCIHMTIQTAVLIETLVDLGAQVRWSSCNIFSTQDHAAAAIAAVGIPVFAWK
                     GETEEEFWWCIEQTINKDGKPWDANMILDDGGDVTGIVHEKYPQMLDHIHGISEETTT
                     GVHRLVEMLKKGSLKVPAINVNDAVTKSKNDNKYGCRHSLNDAIKRATDHLLAGKKAL
                     VIGYGDVGKGSAASLRQEGMIVKVTEIDPICAMQACMDGFEVVSPYKNGINTGELADI
                     NQELLATTDLVVTTTGNVNVCDAAMLQSLKNAAVVCNIGHFDSEIDTAFMRANWQWEE
                     IKPQVHKVYRNKATKDHLILLSEGRLVNLGNATGHPSRIMDGSFANQVLAQMYLYERQ
                     FAKLPAAQKAEHIYVRVLPKKLDEEVARDMVEGFGGVLTQLTTQQAEYIGVEQDGPYK
                     PESYKY"
     gene            complement(6868..8022)
                     /gene="metK"
                     /locus_tag="PKZ52_06720"
     CDS             complement(6868..8022)
                     /gene="metK"
                     /locus_tag="PKZ52_06720"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNG59501.1"
                     /translation="MSEYSVFTSESVSEGHPDKLADQISDAVLDAILKDDPNARVAVE
                     TLVKTGMAIVAGEVRTSTYVDLEDLIRQVILDIGYNSSDVGFDGASCAVLNAIGKQSA
                     DIAMGVDEAANKDLGAGDQGLMFGYATNETPVLMPAPIFYAHRLVEKQAQLRKNGTLP
                     WLRPDAKSQVTLRYENGLPVAIDAVVLSTQHSPDVSQSDIHEAVRELIINQVLPQEWL
                     HAGTQYHINPTGQFIIGGPVGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLADKCEIQVSYAIGVAEPTSISLNTFGTGKIADAEIIKLVREH
                     FDLRPQGLIAMLDLKRPIYQQTAAYGHFGRELPNFTWEKTDRAAALKKAL"
BASE COUNT         1948 a         1813 c         2033 g         2304 t
ORIGIN      
        1 tgccaaattc aacgacgcta gggcgagtga ggtaattttt cagaggttcc ctagctgtac
       61 ggaagcaaag aatagacatc gtttcagacc gtcaaaaaga gggacctttt tgacgagcct
      121 acacggatgt aattacggcg tgtttgaaat gatgtgtatt ctttgctgct gcctaggtag
      181 taattacctc ttttattcat gggggtagag ttaacgaggg gatatcatgg gcaattggtg
      241 gcatagaatt acgcagtcgg atgcatttag cggcatttta ttaatgcttg cggctgtttt
      301 ggccctgttg gcggctaata gtcccttggc gggcctgtac aatcaatttt tatccttaaa
      361 acttgttatc gccatcggcg actttagcat cgctaagcct ttgttgctgt ggataaacga
      421 cggcctgatg accttgtttt ttttaatggt aggcctggaa ttaaagcgcg agatattggt
      481 gggggaatta agtcaggcct ccaaagtggc ttttccggct gccgccgcct tgggggggat
      541 gctggtgccc gcgcttattt atacggcgtt taattatcac gaccccattg cccttaatgg
      601 ttgggcaatt cccaccgcca cggatattgc ttttgcgctg ggcgtattga gtttattggg
      661 gagccgagtt cctgctggcc ttaaattatt tttagtcacc ctggctattt ttgacgatat
      721 tggagccatt gtgattattg cgctgtttta caccgcgaag ctgtcctggt tggccatttc
      781 ctgtgccgct attggcttaa cattactggc atttatgaat tggcgcggcg gcattaatcg
      841 cacggcctat ttattaattg gcttatttgt ctgggggagc ctgttaaaat ccggtgtgca
      901 cgcaaccctt gccggcgtgg ccattgcact gctaattccc tataaaggaa ttgatagcag
      961 cgggcgggag caaacgccgg tggcggatat agagcatgat ttacatagcg tggtgaacct
     1021 aggtgtactg cctttattcg cctttgccaa tgccggttta tcttttgtgg gattaaatcc
     1081 cgctgccctt atcgccggtg ttcccctggg gatttgtttg ggcttggtac tgggtaaaca
     1141 attgggtgta atgttatttg cctggcttgc tgttgcatta aaaattggcc gcttgcccag
     1201 tgactgcaac tggagccaaa tttacggtgc ggctttattg tgtggtgtgg gttttaccat
     1261 gagtttattt attggctccc tggcgtttgc agaagccgct gtgagtgagc ctatgcatga
     1321 gcgtttaggc attttagtgg gctcagtggt gtctgctgta ctgggttatt tttggttggc
     1381 gcgggtggca aggccaaata gtgagtagaa gtaataattc attacagttg ctagatgatt
     1441 aatttcaata attttaaaag aggaagtgtg tatggaagtt gtaatggcct atcaatcgtt
     1501 aaattctaat tccagcaccg agcacgatgt ggcggccagt acgcggcagg catttcagcg
     1561 tgcggcgcaa cacaccttag cgcctatgta tcctaaaggc acaggtgtgt tgcgctaccc
     1621 atttaataat gatttagatc cctcccttat tttacaaggg ccagcggagc tagtgccggt
     1681 tggggataaa aattatttgg atgataaaaa acccagtaaa aaacgtaaat ccgacgaggc
     1741 ggatactgta gatgatgtgc aatttgccgc cgccaaatcg gaaatgctca gcatggttga
     1801 aggcaaggcc cgccagctat accagtttgc ttatcgagaa gttggtgcga ctattgatgt
     1861 gatagttttt ggtgagctga actcaaccca cgcccaatgg caatccatca acaaagaagc
     1921 gggccaaaaa attcatgtat ccgtgcggcc taaagcctgt aattgtttta gcattcacgg
     1981 cccctcgggc accacacagt caattgcgga aggcgagggc tggtgcgctg tgcgctgcaa
     2041 aggtgtatta gccgtttttg tgcacgtccc taatagcctt gccgccaata aagatgatgc
     2101 aaccacattc tacaagtcca ttaaaaatat cttgctcaac acgcccaacg gcggtgtggt
     2161 ggatgtggtg attggcgata ctaaccaacc ccgcgaaact tttagcccgg aggtaattag
     2221 cagtggtatg gatcaaacct ttgtggatgc ccacagtgga gccattttat tgagtgacac
     2281 ctgggcggaa aaacccgtga ttcacaaggg cactaattcg gccaacagta aaaaatttga
     2341 tgtggcggtt tataacacca gcacggttaa aaaaattgaa gtgaaatata tatcgcagtt
     2401 ttcatttgcg ggtagaagtg cggtggctta tacggatcat atgggggttt tggtgaaggt
     2461 ggataagaag tagataccat ctctccccat actgccgcta gcaactagat ttaatgcttg
     2521 cggcagtatg gggagagatg gtatctacgc ctaactgagg tcttgatttg gctagcttaa
     2581 taataggtgg gcagctctgt atcttctgaa gcctcaacct ttaggggcgt ccaatggatc
     2641 tagccaaggg gtgccgctta tagcaggcaa agcttcaagc agcgttatgt ctgattttgt
     2701 tttggctttg atcatacgta caacatctaa aaatgttttt tctgtccact gctctggggt
     2761 tcctacaagt gaataggtgg cggatcgcgc tacctgtgta agtccacctt cttctttgct
     2821 gaagacagca tataaatcta aatgatatcc acctttgtag gcatataaac acgcgctgag
     2881 ttggagattc cctgcacccc ccgtatttcg gttggcttta gctgtccatc gcgcgccatc
     2941 acaggtggca accttaatgc caatagaacc tgctccacca gcaaaagcag aaagattgga
     3001 gcctgccaag ggattaacaa gtttaaatcg tccaggctta tcgggtattt catttgagtc
     3061 tgggataggc gtcgcttcat cagcattatt gacgttccga cccaatccat tactggctgc
     3121 actaataata tccggaagtt gcgccgttgt tttaatatcg aatatacgat aatactcaac
     3181 ggtttttgtt ttagctgtca acatgttatt ggtggtgccg caacctgata ataaagcgca
     3241 gcctaaaata agcgaaactg aaattttcat cgttaatcct atttggaatt attaaaattg
     3301 cagcaaagtc gaatttgcag attggcgaat gccgaaggga tgccaataca attgaattgt
     3361 attcttggtt aagaagcaat cggtttataa gggtaatgaa taccgttaat gtaaacaacc
     3421 cttataaata attgtgtcag tggcaaagtg accatggctc cgagcgagcg gcatattacc
     3481 agatgaaaat tcccgtttca aattaatctg gatacacaaa atgcatccag ctcgtaggct
     3541 gggcagtcat gccctgcacc atatttcttc tcaagcgccc tgtatataaa aaacctatcc
     3601 aaaatttgtt atcgaagaca tcgctgtgtt gggcattact gcccaacgtt ttttccaaaa
     3661 ctatctcaaa tccccccact gatattgcac caagcttaac gccaccaaag gcgcagtttc
     3721 cgtgcgcaaa attcgcggcc ctaaaaccag tggattaaaa ccctgttgct cggccagctt
     3781 aatttcatcc tcacttaatc ctccctccgg cccaattaat aaacacacgg aatgaggtgt
     3841 gctatagctg tttaaggctt ggttggcgcg gtggtggagg acgagtttta aatctgcagc
     3901 tactgtaggt aaaaattcgc ttaatgtttg cggtgcgcgt agctgcggca ataaattgcg
     3961 gccattttgt tcacagctgc taattaacac ctgcagccag tgttcggttt ttttactttg
     4021 ccgggtggca tccaggcgca cttcggtgcg ctgggtaaat agcggataaa tttgtgtaac
     4081 gcccaattca caggcttttt gcaagacaaa ttcaaagcgc tcgcccttgg atataccaat
     4141 agctaggctg caattaagcg gggattggtg attgtcggcg gtaaaatcca ctaaacgaat
     4201 aacgcagtta tttttacctg cggtgataat ttcagcgttg tggctgcccc cttcaccatt
     4261 aaatacaatt aatggccgcc ctgcctccat acgcaatacc cgtgtgaggt aatgggcctg
     4321 ggcttccggc aagctgatat ccactcctgc agtcagcggc agggccacat atacgcgggg
     4381 gatgcgcatt agttttccca gcctaggttg cgcaaaatgg tggcggtggg gtctgtttgg
     4441 ttcatggtgt aaaaatgcag gccgggggct tcgttatcca gcagggtttc acataattcc
     4501 gtcactatat ccacaccaaa ggcttttaag ctttcgttgt cgtcgccaaa actttccaag
     4561 cgtttgcgca gccagcgggg aatttctgcg ccgcaactgt cgctaaaacg agagaggttt
     4621 ttgtaattgg tgatgggcat aatgccgggc actatgtcct ggtgaatacc ctgggcgcgg
     4681 cattgttcga caaaataaaa ataggcatcc gggttgtaaa aatattgggt aatcgcggaa
     4741 ttggcgcccg cgtcgaattt gccttttaaa aagccaatat cgctaataaa gcttgtggat
     4801 tctgggtgga tttccgggta ggcggccact tcaatatgaa aataatcgcc aaagtgctga
     4861 cgaataaagg ccactaattc attggcataa accatgtgca aaccgccgcc catgccggag
     4921 gggcgatccc cccttaatgc caccaagcgg ttaatgccgt ggctttggta gtcttttaac
     4981 agtgccaaaa gtgcttccgg ggagtcgccg ccgaaggaga ggtggggggc aacttcttgg
     5041 ccagcttgtt tgattaactg cactatgcct ttggtgtgct cgcgggtgga gccgcctgca
     5101 ccataagtga cggaaaaaaa ttccggttta aaagccgtta attcttgttg cacctgaatt
     5161 aatttatccc ggccttggtc ggttttggcg gggaaaaatt caaagctgag tcgaatgtta
     5221 tcggtcatgg ggattctcgt gtcgagtgtt cgacagtttt tgccgtgttg ttcggggtgt
     5281 ttaaaagtgt tgtttgttag gcatttgcac gcggtgggca ataaattgcc caccctacat
     5341 ttgctcccgc gtttcttgcg tcctttttcg cgtactcact ttttcttgtt cttactatct
     5401 cgtgctccca cgcgggagcc tgggaacgag gtgttgcttg tcgatggaaa ttaatattta
     5461 tagctttcgg gtttgtaagg cccgtcttgc tccactccta tatattcagc ttgctgagtg
     5521 gttaactgag ttaacacacc gccgaagcct tccaccatat cgcgggccac ttcttcgtcg
     5581 agttttttcg gcagtacgcg cacataaata tgctcggctt tttgcgctgc gggtaattta
     5641 gcaaactggc gctcgtacaa atacatttgt gcgagcactt ggttggcaaa ggagccgtcc
     5701 ataatgcgtg atgggtggcc tgtggcattg cctaaattta ccaagcggcc ttcggataac
     5761 aaaattaaat gatccttagt ggctttattg cggtacacct tgtgtacttg cggtttaatt
     5821 tcttcccact gccagttggc gcgcataaat gcagtgtcaa tttcgctatc aaagtggccg
     5881 atgttacata ccactgcggc atttttcagg ctttgcaaca tggcggcatc gcacacgtta
     5941 acattgccgg tggtggtaac aactaaatcc gttgtggcta ataattcttg attaatatcg
     6001 gccaactcac cggtattaat gccgttttta tagggcgata ctacttcaaa accgtccata
     6061 caggcttgca tggcgcagat ggggtcgatt tcagtgacct taacaatcat accttcctgg
     6121 cgcagggagg cggcggagcc tttgcccaca tcaccataac caattaccag ggcttttttg
     6181 cccgctaaca aatggtcggt ggcccgttta atggcgtcgt ttaaactgtg gcggcagccg
     6241 tatttgttgt cgtttttact tttggtaacc gcgtcgttca cattaatagc gggcactttt
     6301 aagctgcctt tttttaacat ttccaccaag cggtgaacgc cagtagtggt ttcttctgaa
     6361 atgccgtgaa tatggtcgag catttgcggg tatttttcat ggactatacc ggtcacatcg
     6421 ccgccgtcat ccaaaatcat attggcatcc cagggtttgc cgtctttatt aatggtttgt
     6481 tcaatacacc accaaaattc ttcttcggtt tcacctttcc aggcaaatac gggaataccc
     6541 acagcggcaa tggccgcagc ggcgtgatct tgggtggaga aaatattaca ggatgaccag
     6601 cgtacttggg cacctaaatc caccagggtt tcaattaata cggcggtttg aatggtcatg
     6661 tgaatacagc cgataatttt ggcaccagct aatggtttgc tggcttggta tttacggcgc
     6721 agggccatta aagctggcat ttcgccttcg gcaatttcaa tttctttgcg gccccaatcg
     6781 gcaagggaca tatcggcaac tttgtaatcg gtgaagctca taaaaaattc ctgtgaataa
     6841 agagtgtggg tatacgcagt tggatgctta cagggctttt ttcagcgccg ctgctcgatc
     6901 agttttttcc caagtaaaat tcggcaattc acggccaaaa tgcccgtagg ctgctgtttg
     6961 ctggtaaatg gggcgcttta aatccaacat ggcgattaaa ccctgtgggc gcaggtcaaa
     7021 atgctcgcgc accagtttaa ttatttcggc atcggcaatt ttgccagtgc cgaaggtatt
     7081 taatgaaata gatgtgggtt cggccacacc aatggcgtaa ctcacttgaa tttcacattt
     7141 atcggcaagg cctgcggcta caatattttt tgccacataa cggcctgcgt aggcggcgga
     7201 gcggtctact ttggatgggt ctttaccgga aaatgcgccg ccgccgtggc gggccatgcc
     7261 gccgtaggta tccacaataa ttttgcggcc ggttaaaccg cagtcgccca ctgggccgcc
     7321 aataataaat tggccggtgg gattgatatg gtattgggtg cccgcatgta accattcctg
     7381 cggtaatact tggttaataa tgagctcgcg caccgcttcg tgaatatcac tttgggagac
     7441 atccgggctg tgttgggtgg agagtacaac ggcgtctatg gccaccggta aaccattttc
     7501 ataacgcagg gttacttggc ttttggcatc tggccgcaac cagggcaatg tgccgttttt
     7561 gcgcaattgg gcttgttttt ccactaatcg gtgggcgtaa aaaatggggg cgggcattaa
     7621 tacgggggtt tcgttagtgg cataaccgaa cattaaaccc tgatcgccag cgcctaaatc
     7681 tttgttagcg gcttcgtcta cacccatggc gatatccgct gattgtttgc cgatggcatt
     7741 taataccgcg cagctggcgc catcaaaacc cacgtcgctg gagttgtagc ctatatctaa
     7801 aatcacttgg cgaattaaat cttctaaatc cacataggtg ctggtgcgca cttccccggc
     7861 cactatggcc ataccggttt ttactagggt ttctacggcc acgcgcgcat tggggtcgtc
     7921 ttttaaaatt gcgtctaaca ctgcatcaga aatttgatcg gccaatttat ctgggtggcc
     7981 ttcagagaca gattcagagg taaaaactga gtattcggac ataaacttac actcccgtgg
     8041 gtttaaaaaa ttggtgtact tgaatttgaa agccattgcg cagagctaaa taactgct
//
LOCUS       DAOQGS010000132         8094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4226_length_8094_cov_4.536634, whole genome shotgun sequence.
ACCESSION   DAOQGS010000132 DAOQGS010000000
VERSION     DAOQGS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_8094_cov_4.536634"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1581
                     /locus_tag="PKZ52_06725"
     CDS             142..1581
                     /locus_tag="PKZ52_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNG59502.1"
                     /translation="MRIILSGILLGIIGAFSASVVWSADDGAVLYGKHCQQCHGAQRE
                     GGVGPNLADGVWGIKEPTVAAISQIIRDGSVNKAMPPWGQVLSGDEIERLATWLLNGA
                     VKSPSAPVAPQLNLSADSELQAVRMPKDFVISIFADQLANPRSLAVDADDTVFVGSRK
                     AGSVIALKDANQDGVAEQKYTLATGLHEPTGVALHQGDLYVAETSRILRFKQARSQLE
                     TPQPFDVIKSDLPDKAWHGARAIEIGPDHKLYIPIGAPCNVCDVEDGPFGKIWRMNLD
                     GSGFEPVVKGVRNSVGLAWHPLTRELWFTDNGRDELGDNTPSCELNHAPQAAMHFGFP
                     YCHGGSILDPEWGKGKSCAGYSAPVALLGPHVAPLGLAFYTADQFPSIYKNQLFIAEH
                     GSWNRTQKIGYRISLVTLNGNALVSDTVFADFLQGDTVLGRPVDIAFLKDGSMLVSDD
                     DKGRIYRIRYTGEKPGVIRSLLNSVKAAL"
     gene            1676..3037
                     /gene="glmU"
                     /locus_tag="PKZ52_06730"
     CDS             1676..3037
                     /gene="glmU"
                     /locus_tag="PKZ52_06730"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNG59503.1"
                     /translation="MLEIIILAAGKGTRMKSNLPKVMHLLAGRPLVQHVIDRARELSA
                     GKIHLVVGHGAQKVEAALAAADLTFSLQAQQLGTGHAVMQALDKLQADSRVLILYGDV
                     PLIRSATLAQLVELAGDNSMGLLTVNLANPTGYGRIVRAADGAVTAIVEQKDASAEQL
                     LITEGNSGVMAVTAKQLRRWLPQLSSQNAQGEYYLTDVIALAAAEGTQIKTVLAADEI
                     EVLGINDRAQQAQLERAFQLQQARVLMQQGLTLLDPTRFDVRGRLTVGQDCIVDINCI
                     FEGDCILEDGVEIGPNCVIKNARIGAGTHIYANSTVEDSQIDAACSVGPYARLRPGTH
                     LKTQAKIGNFVETKKAVIGEGSKVNHLSYIGDAFLGDDVNVGAGTITCNYDGVNKHVT
                     QIGNNCFVGANSTLVAPIKVGDNATIAAGSTITTSVHKDELVVARTRQRHIEGWQRPA
                     KKK"
     gene            complement(3112..4239)
                     /locus_tag="PKZ52_06735"
     CDS             complement(3112..4239)
                     /locus_tag="PKZ52_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNG59504.1"
                     /translation="MNYSTLSAVLLAALVTANAAAYDPKAGPQVEVKTGEWWNEPYPQ
                     GFDAKQLANKQSEIKVAGKLLVDEAGNTVVLRGVNIGDPDKLAKQGRWDKRVFEGAKS
                     FGANIVRLPIHPIAWRSRGSAEYLKLIDQAVIWANELNLYLIVDWHSMGNITKELYFH
                     PMYVTNQQETREFWRTISSRYAGLATIAVYELFNEPTINGGKLGEVDWNEWKQFNEEI
                     ISIIQAHDKNIVSLVAGFNWAYDLSKVKEAPINREQVAYAVHPYPQKVKDGPLAETWE
                     KQWGYVADTYPIIATELGWMRADQKGAHVPVINDGSYGPIISQYLEKRGISFTVWCFD
                     PDWPPQMISDWNFTPTEQGKFFKEYMLNAAKKDAKAQKTKK"
     gene            complement(4297..4971)
                     /gene="radC"
                     /locus_tag="PKZ52_06740"
     CDS             complement(4297..4971)
                     /gene="radC"
                     /locus_tag="PKZ52_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNG59505.1"
                     /translation="MSIKDWPASERPREKLLQYGPSALSDAELLAIFLRTGISGKSAV
                     DLAREQLAHFGSLRALLAADLSAFCRAEGLGPAKFTQLQAVVEMARRHLAESLSERCV
                     LSSPQAVKRYLTAQLRHRPAEAFACLFLDNQNRLIVFETLFQGTIDGASVYPREVVRR
                     VLAHNAAAVIFAHNHPSGVAEPSRADHAITQRLKDALALIDVRVLDHLIIGEQEPLSF
                     AEQGWI"
     gene            5128..6339
                     /gene="coaBC"
                     /locus_tag="PKZ52_06745"
     CDS             5128..6339
                     /gene="coaBC"
                     /locus_tag="PKZ52_06745"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNG59506.1"
                     /translation="MNILLGISGGIAAYKAAELTRLLKKHGAQVRVVMTPAAQEFITP
                     LTLQALSGEPVHTELLNPAAEAAMGHIELARWADAIVIAPASADVLARLSAGMANDLL
                     TTLCLASTAPLFLAPAMNQAMWRHPATQANCAQLQARGTYLLGPAEGEQACGDTGPGR
                     MLEPSAIVAHLVQWASAQQGLPNLTGQHWLITAGPTREALDPVRYLTNHSSGKMGYAL
                     ADAAARAGARVTLVSGPTALAAPPAVQRIDVQSALEMLVACDEVLPGVDVVVAAAAVA
                     DYRPAEVAGQKIKKSADALSLALVKNPDILAHLASRLRGLKPRCFVMGFAAETQHLHS
                     HAQAKLQAKGLDAIAANDVSLPGLGFNSDNNALLLIDRQGSTTLNPRSKGKLAEAVVA
                     WVAERFPHDLS"
     gene            6350..>8094
                     /locus_tag="PKZ52_06750"
     CDS             6350..>8094
                     /locus_tag="PKZ52_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNG59507.1"
                     /translation="MNSSLKRTKKGDKPKKTKALPIKAQVLSWVAEHPQKAFVALAVA
                     VMLVLDAASGYFAWQRLVVQERAEQLDKLSQQQARKLSVNLGNFMDATQKKVAFFAQN
                     AELAASLTRHDELALKEFHRAIQNQLPKAEDIRLIPVGEAQLDQSAKIPISFLELQLI
                     KAAEQRQPQKIEAVKLPIGWRVHLILPVPSQADQPVAGTLMITLSMDELEQALVEGVA
                     GLGQISLLQLYDKTNTHTLYTTGQGSLPPSVRREVHATAWLVEFLPNESLAAQIEGNH
                     FMLIFEAVIAFLVSMLLAVGLGRLLANRWEAKQVAQVGREETTGGKTKDINTVRIHSG
                     HEALLGLNISKEVKEANNTTAASAESEAPDLVPAEIFRAYDIRGVAEEQINKELAYKI
                     GQALGSEALDAGQGTLVVAKDARTHSPLLAEYLIRGIISTGCKALNIGTVPTPLLYFA
                     TETLGYTQSGVVVTASHNPANYNGFKLVLNGKARMEDDIQAVRRRIEARDFRMGLGQE
                     EHLDIMPAYIDAIYSDVALAGDVSIVLDAGNGVTGKVAPRLFEELGCQVTSLYCDLDG
                     TFPNHGPDPTVEANLA"
BASE COUNT         1751 a         2074 c         2374 g         1895 t
ORIGIN      
        1 ctcctctcgc tactttcacc attgctcaca gctctttccg aataactccg tggtttgttc
       61 ttccatcgat ccgttggacg caattatttc ccctataatc cgactccaat cgccttagtg
      121 gcagtttttg agcgcgtagt gatgagaata attctgtctg gaatattact gggaataatc
      181 ggggcgttta gtgcaagcgt cgtctggtct gcggatgatg gcgcagtgct ttatggtaaa
      241 cactgccagc agtgtcatgg cgcccagcgt gaaggcggtg tggggccgaa tttggccgat
      301 ggagtttggg gtattaagga gccgacggta gccgcaatta gccagattat tcgcgacggc
      361 tcggtcaata aagccatgcc gccttggggg caggttttat ccggcgatga gattgagcgc
      421 ttggcaactt ggctgcttaa tggcgcagtt aaatcaccct ccgcacctgt cgcgccgcaa
      481 ttaaacctaa gtgcagacag cgagttgcag gctgtgcgca tgcctaagga ttttgttatc
      541 agcatttttg ccgatcaatt ggcgaacccc cgcagcttgg cagttgatgc ggacgacacc
      601 gtatttgtcg gcagtcgcaa ggcgggcagt gtaatagccc ttaaggatgc caatcaggat
      661 ggagttgctg agcaaaaata tacgcttgca actgggctgc acgaacccac gggcgttgcg
      721 ctccatcagg gtgatttata tgttgctgag accagtcgca ttttgcgctt taaacaagcg
      781 cgctcacagt tggaaacgcc ccagcccttt gatgtcatca aaagcgattt gcccgataaa
      841 gcctggcatg gcgcgcgggc tattgaaatt ggcccggacc ataaattgta tattcccatc
      901 ggcgcgccct gtaacgtgtg tgacgttgaa gacgggccat ttggcaaaat ctggcgcatg
      961 aatctggacg gcagtggttt tgagccggtg gtaaaaggtg tgcgcaattc ggtcggtttg
     1021 gcttggcacc cgctgacacg ggagctttgg tttaccgata atggtcgcga tgagttgggg
     1081 gataacacgc cttcttgcga gctaaaccat gccccccaag ccgctatgca ttttggtttt
     1141 ccctattgcc atggcggcag cattttggac cccgagtggg gcaagggaaa aagttgtgca
     1201 ggttacagcg cgccggttgc cttgcttggc ccccatgtgg cgccattggg gttggcgttt
     1261 tatacggctg atcaatttcc ttccatctat aaaaaccaac tgtttattgc tgaacatggc
     1321 tcttggaacc gcacgcaaaa aattggctac cgaattagtt tggtgacact caacggcaac
     1381 gcgttggtgt ccgatacagt atttgctgat tttttacagg gcgatacggt gttggggcgg
     1441 ccggtggaca tagcgttttt aaaggatggc tctatgctgg tgtccgatga cgacaaaggg
     1501 cgcatctacc gcattcgcta cacgggtgaa aaaccaggtg taataagaag tttactcaac
     1561 agtgttaaag cggctcttta aatgagtctg cggactgtat tgttgcgtgt gcctaggcgc
     1621 tggggtgtgc catcagtaga ataggctgtt gattgagtta cccactggat tttttatgct
     1681 tgaaattatc attttagcgg ccggtaaagg gacgcgtatg aagtctaact tgccgaaggt
     1741 tatgcacctt ttggcgggcc gccctctggt tcagcatgtt atcgaccgtg cgcgggaatt
     1801 atcggcgggc aaaatccact tggtggtcgg ccatggcgcc cagaaggtgg aagcggcctt
     1861 agctgcggcc gatttaacct tcagtttgca ggcccaacag ttaggcactg ggcatgcggt
     1921 gatgcaagcc ttggataaat tacaggccga cagccgagta ttaattttat acggcgatgt
     1981 gccgttaatt cgcagcgcaa cccttgcgca actggttgag ctggctgggg ataacagcat
     2041 gggcctgctt acggttaatt tggctaatcc aaccggttat ggccgcattg tgcgcgcagc
     2101 tgatggtgca gtaacggcaa ttgtggagca aaaagacgcc tccgctgagc agttattaat
     2161 tacggaaggc aatagcggtg ttatggcggt gacggcgaaa caattgcgcc gctggctgcc
     2221 gcagttatcc agccaaaatg cccagggtga atattattta acggatgtga tcgcccttgc
     2281 ggccgctgaa ggcacgcaga ttaaaacggt tctggccgcc gatgaaattg aggtgttggg
     2341 gattaatgat cgcgctcagc aggcccaatt ggagcgcgcc tttcaattac agcaggcccg
     2401 cgtgcttatg cagcaggggt taacgctgtt agatcctacg cgttttgatg tgcgcgggcg
     2461 attaaccgtg ggccaggatt gtattgtcga tattaattgt atttttgaag gtgattgtat
     2521 tttggaggac ggagtggaaa tcggccccaa ttgtgtaata aaaaatgccc gcattggcgc
     2581 gggtacccat atttatgcta acagcacggt ggaagacagc caaattgatg ccgcctgcag
     2641 tgtaggcccc tacgcacgcc tgcgccctgg cacccactta aaaacccaag ccaaaattgg
     2701 caattttgtg gaaaccaaaa aagcggttat tggtgaaggc agcaaggtta atcatctgtc
     2761 gtatattggc gatgcgtttt tgggtgatga tgtaaatgtg ggggcgggca ccattacctg
     2821 taattacgat ggcgtgaaca aacatgtcac gcaaattggc aataattgtt ttgtcggcgc
     2881 caattcaacg ttggtggctc ccattaaggt gggcgataac gccaccattg cggcgggttc
     2941 caccataacc accagcgtgc ataaagatga gcttgtggtg gcgcgcacac ggcagcggca
     3001 tatagaaggt tggcagcggc cagcgaagaa aaagtagcga gaaaatagcc gccaataaaa
     3061 aagccccatt tggggctttt ttattgctgc tgatttaaaa gacctggcgg tttatttttt
     3121 tgttttttga gctttggcgt cttttttcgc tgcgttaagc atgtactctt taaaaaattt
     3181 gccctgttcg gtgggggtaa aattccaatc gctgatcatt tgcggtggcc agtctgggtc
     3241 aaagcaccag acggtaaatg aaataccgcg tttttctaaa tattggctga taatcgggcc
     3301 atagctgcca tcgttaatca cgggcacgtg ggcgcccttt tgatcggccc gcatccagcc
     3361 tagttcagtg gcgataatag gataggtgtc cgccacataa ccccattgtt tttcccaggt
     3421 ttcagctagg gggccatctt taactttttg cggatacggg tggacggcat aggcgacctg
     3481 ctctctgtta ataggcgctt cttttacttt ggaaagatcg taggcccagt taaaacccgc
     3541 caccagggag acgatatttt tatcgtgggc ctggatgatg gaaattattt cttcgttaaa
     3601 ttgcttccac tcgttccagt ccacttcccc taatttgccg ccattgatgg tgggctcatt
     3661 aaataattcg tataccgcga tggtggcaag gcccgcgtag cggctagaaa ttgtgcgcca
     3721 gaattcgcgg gtttcctgct ggttggtgac gtacatgggg tggaagtaca gctccttggt
     3781 gatattgccc atggagtgcc agtccacaat taaatagagg ttcaattcgt tggcccagat
     3841 gaccgcttgg tcgatgagtt ttaggtattc ggcgctgccc cggctgcgcc aggcgatggg
     3901 gtgaatgggc aggcgcacaa tattggcacc gaaggatttg gcgccttcaa atacgcgttt
     3961 atcccagcgg ccctgtttgg cgagtttgtc cgggtcgccg atgttcacgc cccgcagcac
     4021 cactgtgttg cccgcctcat ctaccagcag tttgcccgct actttaattt cgctctgttt
     4081 gttggctagc tgtttggcat caaagccttg tggataaggc tcattccacc attcacccgt
     4141 tttgacttcg acttgggggc cagctttagg atcgtaagcg gcggcattgg cagtgaccag
     4201 tgccgcaagt aaaacagcac tcagggttga gtagttcatg gattctccaa ttgtgggttg
     4261 ggggaggcga gcctcaatta ttttgtgtta ttgctgttat atccagccct gttctgcaaa
     4321 cgacaggggc tcttgttcgc ctatgatgag gtgatccaag acccgcacat caatcaaggc
     4381 gagggcgtcc tttaaacgct gggtgatagc gtgatcggcc cggctgggtt cggccacccc
     4441 ggaagggtga ttatgggcga aaattaccgc cgcagcattg tgggccagca cccggcgcac
     4501 cacctctcta gggtagacgc tggcaccgtc aatggtaccc tgaaagaggg tctcgaatac
     4561 tataaggcgg ttctggttat ccaaaaacaa acaggcgaag gcctcggcgg ggcgatggcg
     4621 cagctgggcg gtgaggtagc gtttgaccgc ctggggcgag ctgagcacac agcgctcgct
     4681 taggctctcc gccaggtggc ggcgggccat ttccaccacc gcttgcagct gggtgaattt
     4741 ggccgggcct aggccttccg cccggcaaaa ggcgctgagg tcggcggcga gcagggcccg
     4801 caggctgccg aagtgggcga gctgttcgcg ggctagatcc acggcggatt taccgctgat
     4861 gccggtgcgt aaaaaaatcg ccagcagttc ggcgtcggag agggcgctgg ggccgtattg
     4921 aagcagtttt tcgcggggcc gctcgctggc gggccagtct ttaatggaca tgctgtcatt
     4981 ccttaatctg tgcaactaga gggttggagg ccccaagcca aaggcgccaa aagtttgccc
     5041 tgaggtttac tccctaaaat ggtatcttaa gcgggttttt actcaattgg gaatctcacc
     5101 tccgtgcaca ctgacgcagc tttgcgcatg aacattttgc tgggtattag cggtggtatt
     5161 gccgcttata aagccgctga actcacccgc ctactgaaaa agcacggcgc ccaggtgagg
     5221 gtggtgatga cccctgcggc ccaggaattt atcacccctt taaccctgca agccttaagt
     5281 ggtgagcctg tacacaccga acttttgaac ccggcggcgg aagcggccat ggggcatatt
     5341 gagttggctc gttgggctga tgctattgtg attgccccgg ccagtgccga tgtgctggcc
     5401 cgtttaagtg cgggcatggc caatgacctt ttaaccaccc tgtgtttagc cagtacagcg
     5461 ccgctgtttc tggcccccgc catgaaccaa gccatgtggc gccacccggc cacccaggct
     5521 aattgtgcgc agttgcaggc ccgcggcacc tacctgttag gcccagccga aggcgagcag
     5581 gcctgtggtg acacagggcc tgggcgtatg ttggagccct ctgccattgt tgcgcacctg
     5641 gtccaatggg caagtgcgca acagggcttg ccgaatttaa ccggtcaaca ctggctgatt
     5701 accgccggcc ctacccggga agcgctggac ccggtacgtt atttgacgaa ccacagctcc
     5761 ggcaaaatgg gctatgccct tgcggatgcg gctgccagag cgggcgctcg ggtgacctta
     5821 gtgtccggcc ccacggcatt agccgcgccc ccagcagtgc agcgcattga tgtgcaaagt
     5881 gcgctggaaa tgttggtcgc ctgtgacgag gtattgccag gggttgatgt ggtggtcgcc
     5941 gccgctgccg tagcggatta ccgcccagct gaggtggctg ggcagaaaat aaaaaaatct
     6001 gccgatgccc tgagccttgc cctggtgaaa aacccggata ttttggccca cttggccagc
     6061 cgcttgcgtg gtttaaaacc gcgctgtttt gtcatggggt tcgcggctga aacccaacac
     6121 ttgcacagcc acgcccaggc gaagctgcag gcgaaggggc tagatgccat tgctgccaat
     6181 gatgtgtcgc tgccggggct gggttttaat agcgataata acgccctgct gcttatcgat
     6241 agacagggct caactacact caatccacgc agtaagggga agctggccga ggcggtggtc
     6301 gcctgggttg ccgagcgttt cccacacgat ttgtcctgag gagtccttcg tgaattcgtc
     6361 gttaaaaaga acaaaaaaag gcgacaaacc caaaaaaacc aaagccctgc cgatcaaagc
     6421 gcaggtgctt agctgggtgg cagagcatcc gcaaaaggcc tttgtggccc tggctgtggc
     6481 cgtaatgtta gtgctggatg cagcctcagg gtatttcgct tggcagcgcc tggtggtgca
     6541 ggagcgcgcg gagcagctgg ataaactcag ccagcagcag gcccgcaaac tgtcggtgaa
     6601 cctgggtaat ttcatggatg ccacccaaaa gaaagtggcc ttttttgccc agaatgcgga
     6661 gctggccgcc tccttaaccc gccatgatga gctggcctta aaggagtttc accgcgccat
     6721 acaaaaccag ttgcccaagg ctgaggatat tcgcttgatt cctgtgggtg aggcgcagct
     6781 ggaccaaagc gccaaaattc ccatcagctt tttagagctg cagctgatca aggccgccga
     6841 gcagcgccag ccgcaaaaaa ttgaagcggt gaaactgccc atcggctggc gggtgcacct
     6901 gattctgccg gtgccgtctc aggcggatca gccggtggcg ggcaccctga tgatcaccct
     6961 ttctatggat gaactggagc aggccttggt tgaaggcgtg gcggggcttg ggcagatcag
     7021 cctgctgcag ctgtacgaca aaaccaatac ccacaccctc tacaccaccg ggcagggttc
     7081 gctgccgccc agtgtgcgcc gggaagtgca cgccaccgcc tggctggtgg aatttctccc
     7141 caatgaaagc ctggcggcgc agattgaagg caaccacttt atgctgatct ttgaggcggt
     7201 gatcgccttt ttggtctcca tgctgctggc ggtgggcctc ggccgtttac tggccaaccg
     7261 ctgggaggcg aagcaagtgg cgcaggttgg tcgcgaggag accaccggtg gcaaaactaa
     7321 agatatcaat acggtgagaa tccacagcgg ccatgaggcg ctgttgggct tgaatatcag
     7381 caaagaagtt aaagaggcca acaacacgac tgctgccagc gcagagtccg aagcccccga
     7441 tctggtgccc gcggaaatct tccgcgccta cgatattcgc ggtgtggccg aggagcagat
     7501 caacaaagag ctggcctata aaatcggcca ggccttaggc agtgaagcct tagatgcagg
     7561 ccagggcacc ctagtggtgg ccaaagatgc ccgcacccat tcgcccctgc tggcggaata
     7621 ccttattcgc ggcattatca gcaccggctg taaagccctc aacataggca cagtgcctac
     7681 gccactgctg tactttgcca ccgaaaccct aggctatacc cagtctggcg tggtggtgac
     7741 cgccagccac aaccccgcca attacaacgg ctttaagctg gtgctcaatg gcaaggcccg
     7801 catggaagac gatatccaag ccgtgcggcg gcgtattgag gcgcgggatt ttcgtatggg
     7861 cctgggccag gaggagcacc tggacatcat gcccgcctac atagatgcca tctattccga
     7921 cgtggccctg gccggtgatg tctccattgt gctggacgcg ggcaatgggg tgacgggcaa
     7981 agtggcgccg cgcctgtttg aagagctggg ctgccaggtg accagcctct actgcgactt
     8041 ggacggcacc tttcccaacc acggcccaga ccctacggtg gaggcgaacc ttgc
//
LOCUS       DAOQGS010000133         8046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4258_length_8046_cov_4.936929, whole genome shotgun sequence.
ACCESSION   DAOQGS010000133 DAOQGS010000000
VERSION     DAOQGS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_8046_cov_4.936929"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..812
                     /locus_tag="PKZ52_06755"
     CDS             102..812
                     /locus_tag="PKZ52_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNG59508.1"
                     /translation="MAIKLNDGLVFAGDTRTNAGVDHVATYRKLFTFTNNSTHSIVLL
                     SSGNLATTQALVELLSRLCHNQPDGLFELPSMFDIASRVGKKLVGLIQEGSEISSYQG
                     VDFSCNLLLGGQVQGEVPRLFHIYPQGNFVEASAETPFLQIGEEKYGKPILDRIIAPT
                     TPLQTALNCALVSLDSTMKSNMTVDMPLDVLVYLRDSFDLSHQRRIDRDDEDFLSMRR
                     YWGTGLRRLLDNMPSFNV"
     gene            813..1586
                     /locus_tag="PKZ52_06760"
     CDS             813..1586
                     /locus_tag="PKZ52_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNG59509.1"
                     /translation="MPRLDQLLVQQGLAHSRTQAQKLIASRCVEVFTQGLWQVCSKPA
                     SQFDAPPALRIAALDELQFASRAGLKLQRALQHLNWPISGSWLDIGQSTGGFTDCLLQ
                     AGASQVIGIEVGKDQLIERLRTDPRVICLEGINGRALPIEKLNPLAPAGFSGAVMDVS
                     FISQTLIHPQLARVLPPGGKLLSLVKPQFEAGPEYLGKGGLINNPAAFEKVQAKIIAS
                     MLQLGFELVDYFDSAITGGDGNREFFVAAINSTHSGTQA"
     gene            complement(2110..4416)
                     /locus_tag="PKZ52_06765"
     CDS             complement(2110..4416)
                     /locus_tag="PKZ52_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59510.1"
                     /translation="MNFIRHSLFTLACVSLVACGSGGSKNKSAPAETTSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSGSTKPDNASSSSSSSSSSSSGVVVSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSGIPVAKALINVGIQAADKTVLLSDKAIIDVKRLGANDQVLSESQFNSASDNS
                     TSTPRLKFNSDVAVDGAKALLVNISYPGFASYSRRLDVQPLIFVDALLSKAPALTSPV
                     KTGTFVLRSGESVEGVQLDLTDSLTITVPAGLLPSQNLTARAASFDPNSPAEAIYFPG
                     QYANLNGEPLTPTAFDFIALANDNGAPLDLIAGEKSVTVSRILPASSCTTLNNLGDSN
                     SLQAGFQVPMYAFDGAKGLWALAGEGTLYNSDGSLLSALNCPAKVWVEGAVAGAVINK
                     QWVSFSFVQKPVAPVTYCASVQINNAQNEKLAGIYGLVSATNAGQFTPAYFTTGVDGN
                     ARIEITAAGATESIPAKLSLINDGVVNADVVLTPNCTNPEPQVVPLNRPRLCQIQGQV
                     TDANGEPLVEYPVVAKAIKNAASAGLFDFAITDKQGIYWLNVACKEEFSLKFTRFFNT
                     ELEVLGVAVDGTVAGKELKDDGQTAYVSSTKVATVATGISTAVYSVSTGELTINLLSS
                     HGSFPLTAKLQVVNAKNEVVAPINASVQDSANLGENSPNWIYGSGQLVQKINLGDGNG
                     LKILGSLTDAKDKITQIGIDIPVAITVK"
     gene            complement(4844..6514)
                     /locus_tag="PKZ52_06770"
     CDS             complement(4844..6514)
                     /locus_tag="PKZ52_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5010 C-terminal domain-containing protein"
                     /protein_id="HNG59511.1"
                     /translation="MKLQQTINKVLTAGGLLTLLVTSACGGPVDGASSSSSSSSSSSS
                     SSSSSSSSSGRTSSSSSSSSSSSSSSSSSTSSSGAINTTCPGNISDAYQQMVRLIPGK
                     IEAEDFDPAGYSDTTTSNEGGAYRTDTGVDIKAISDGYAVGWTVAGEWLEFTVNVARE
                     GEYNLTMRLGAVETGRTLSLSQCDKILIENIAVPQVSAWGEFKTLAMGKITLKGGTQK
                     IRLNITGADGVDIDWIYLGNYSGTIDPVTPPPATEGEPGNYPLGNAPVKSSGCGTDGK
                     ASLLSGGTSVAQGLATSTRLKLTSNNASREYIIDIPADYDKSKPYRLFYTSHWIGSTN
                     TAVATGETGNGGATNWGFYGLRRESLAAKDPAIFVAPQSVGSTWQETDNRYLFDDILK
                     KVSGSLCIDMSRVFATGFSFGGMITYSLSTNHQKSIRAAVGIGPANYNIWLPSPIPKD
                     PIAWMSTTGMSDTTTPWDGGNGRGAKYIAEQRARDNGCTAATIPTWSSSSPSRHVCYD
                     YQGCKSNYPVKACTFNGGHQAAPFDAGSGDNGATTWIASESWKFFRQF"
     gene            complement(7426..7890)
                     /locus_tag="PKZ52_06775"
     CDS             complement(7426..7890)
                     /locus_tag="PKZ52_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNG59512.1"
                     /translation="MGAQNNINRCVMWAAVLFGLLGCASNPQVASHNAREAVIATELA
                     FAKTMADRDFAAFTSYIAQEAVFLQHIPLQGKNKIVEHWKPLYQAPKAPFSWRPEEVE
                     VLESGKLAISTGPVFDPNGKITHRFTSIWRLRPDGKWEIIFDQGNPFCEDKK"
BASE COUNT         2075 a         2136 c         1889 g         1946 t
ORIGIN      
        1 gcgccgctcg cgtaccctga gcggccaccc tcgcagtttg atggacatct gccaaaataa
       61 aagcgtgtgg atatttaggt aatcaactca tgacttattg tgtggcaatt aagctaaacg
      121 acggcttagt gtttgcgggc gacacccgca ccaacgcagg cgtggaccat gtggccacgt
      181 accgcaaact attcaccttc accaacaaca gcactcactc cattgtgctg ttaagctccg
      241 gcaacctagc caccacccaa gccctagtgg aactgctcag ccgcctgtgc cacaaccaac
      301 ccgacggcct gtttgaattg ccatcgatgt ttgatatcgc ctcccgcgtg ggcaaaaaat
      361 tggtcggcct gattcaagaa ggcagcgaga tctccagcta ccaaggcgta gacttcagct
      421 gcaacctgct gctcggcggc caggtgcaag gcgaggtgcc gcgcctattc cacatctacc
      481 cgcagggtaa ttttgtggag gccagcgccg aaacaccctt tttgcaaatc ggcgaagaaa
      541 aatacggcaa gcccatcctc gatcgcatca tcgcccccac cacccccctg caaacggccc
      601 ttaactgcgc cctggtatcc ctcgactcca ccatgaaaag taacatgacg gtggacatgc
      661 ctctggacgt actggtgtac ctgcgcgata gctttgattt aagccaccag cgccgcatcg
      721 accgggacga tgaagacttt ttaagcatgc gccgctactg gggcacgggc cttaggcgcc
      781 tgctggacaa catgccctcc ttcaacgtgt aaatgccccg cctcgaccaa ctcttggtgc
      841 agcagggcct tgcccactcg cgcacccaag cgcaaaaact cattgccagc cgctgtgtgg
      901 aagtcttcac ccaggggcta tggcaagtgt gcagcaaacc tgccagccag tttgacgccc
      961 ccccggccct gcgtatcgcc gccttagacg agctgcaatt tgcctcccgg gccggtttaa
     1021 aactgcagcg cgccctgcaa cacctcaact ggcccatcag cggcagctgg ctggatatag
     1081 gtcaatccac cggcggcttt accgactgtc tacttcaagc cggtgccagc caggtcattg
     1141 gcattgaagt gggcaaagac caactcatcg agcgcctacg caccgacccc agagtcatct
     1201 gcctagaggg catcaacggg cgagcgctgc ccatcgaaaa actaaacccc ctcgcccccg
     1261 caggctttag cggcgcggtg atggacgtat cgtttatctc ccaaaccctg attcacccgc
     1321 agctggcccg ggtcttaccc ccaggtggaa agcttttaag cctggtaaaa cctcagtttg
     1381 aagcggggcc tgagtattta ggcaaaggcg ggctgatcaa caacccggcc gcctttgaga
     1441 aagtacaggc caaaataatc gcatccatgc tgcagttggg gtttgagctg gtggattatt
     1501 ttgacagcgc gattaccggc ggggatggca accgggaatt ttttgtggcg gctattaaca
     1561 gtactcatag cggtacccaa gcctaaaaat acaggtttcc aaataacgcc cgtatttatc
     1621 tttaattctt cagtacgccg taatttatat ttacctgcct tttcgcgtgc ggcgtgtttc
     1681 gcaggttgca acccctcacc acggggctgg tgtgcatttg gatcctgcga ctgcgcgcag
     1741 gatgacacgc tctaaaattt cgcttcctta atatctcatt ctgcacaact caaccgtcat
     1801 cctgcgcgca gtcgcaggat ccattttgtt gagtggcaaa ggcttcagca caccagaatt
     1861 tatttttatc tggtttttca tcgcttacgg tgtgtctgca ataacctgtt gcacccctac
     1921 gggggcctaa atcactcacc taatggcttc ccttcggggc acgaaaaagt gtactgaaca
     1981 gctacattta ccttttttaa caaccaataa aaattcccgc ttcacacaac taaaaacgcg
     2041 ccgaataaca attgttattc ggcgcgttag acgtcgcgtt cactaaaggc aagcgagcgt
     2101 taaaggcaat tatttaacgg taatggcaac gggtatatca ataccaatct gggtgatttt
     2161 atccttggca tcggtgagcg agcctaatat ttttaggcca ttaccatcgc ctaaattaat
     2221 tttttgcacc agctgcccgg aaccgtaaat ccaattgggc gagttttcac ctaggttggc
     2281 gctgtcctgc actgaagcgt taatgggcgc aacaacttca ttcttagcgt taaccacttg
     2341 taatttagcc gtcaagggga aactaccgtg gctgctcaat aggttaatgg ttaattcacc
     2401 ggtgctaact gaataaaccg ccgttgaaat accggtggca accgtagcaa ccttagtgct
     2461 gctcacgtac gccgtttggc catcatcttt taattcttta ccggccaccg tgccatccac
     2521 tgcaacgcca agcacctcta attccgtgtt aaaaaagcgg gtgaatttaa gagaaaactc
     2581 ctctttacac gccacattta accaataaat accctgcttg tcggtaatcg caaaatcaaa
     2641 taaccccgcg ctggctgcat ttttaattgc cttggcgacc acaggatact ccaccaacgg
     2701 ctccccatta gcatcggtta cctgcccttg gatttgacac aaacgcggcc gattcaaagg
     2761 cacaacttgc ggctcgggat tggtgcaatt aggcgtcaga accacatcgg cattcaccac
     2821 cccatcatta attaacgaca atttagcggg gatggattcg gtggcacctg ccgccgttat
     2881 ttcaatacgc gcattaccgt ctaccccggt ggtgaagtaa gcaggggtaa attgcccggc
     2941 atttgtcgcg ctaacaaggc cataaatacc ggccagtttt tcattctgtg cgttattaat
     3001 ttgcacactt gcgcagtagg taaccggcgc cacaggcttt tgcacaaaac taaaactcac
     3061 ccactgctta ttaataaccg caccggcaac cgccccctca acccatactt tggcagggca
     3121 atttaacgcg cttaacaagg agccatctga attatacagc gtgccttcac cggctaacgc
     3181 ccacaaaccc ttagcgccat caaacgcata catgggcact tgaaacccgg cttgcaggct
     3241 attgctatca cctaggttat tcagtgttgt acaactgctc gcaggtaaaa tccggcttac
     3301 tgttacagat ttttcaccgg caattaagtc cagcggcgcg ccgttgtcat tggcaagcgc
     3361 aataaaatca aaggcagtgg gtgttaatgg ctcgccattg agattggcat attgaccggg
     3421 gaaatatatt gcttcggctg ggctgttggg gtcaaaactt gcggccctag ccgttaagtt
     3481 ctgggacggc aataagccgg caggaaccgt aatagtgagt gaatccgtta aatccagttg
     3541 caccccctcc accgactcac ccgagcgcag cacaaaagtg ccggttttta cagggctggt
     3601 gagcgcaggc gctttggata aaagcgcatc cacaaaaatc agcggttgca catccaaacg
     3661 gcgcgaatag gacgcaaaac ctgggtaact gatattaacc aataaagcct tagcaccatc
     3721 aactgctaca tcactattaa attttaatct gggtgtgctg gtggaattat cagaagcgga
     3781 attaaactgc gactcactta acacctggtc attcgcgccg aggcgtttaa catcaataat
     3841 ggctttatca gataacaaca ccgttttatc agcggcctga ataccgacat taattaatgc
     3901 ctttgcaacg ggaataccgc ttgagctgga ggaactgctt gaacttgaag aactgctcga
     3961 actagacgaa ctgcttgaac tagacgaact gcttgaacta gacgaactac ttgaacttga
     4021 agaactgctc gaactagatg aactacttga acttgaagaa ctgctcgaac tagacgaact
     4081 gcttgaactt gaagaactgc tcgaactaga tgaactactt gaacttgaag aactgctcga
     4141 actagatgaa ctgctcgaac tcaccacaac acctgaactt gatgaactgc tggagctgga
     4201 tgaactgctg gcattatccg gttttgtgct accagatgaa ctgctggatg aactactaga
     4261 actggaggag ctactggatg aactggatga tgagctgctg gaacttgacg aacttgtcgt
     4321 ttctgccggc gcggatttgt ttttactgcc gccgctgcca caggccacca ggcttacaca
     4381 ggccaaggtg aaaagtgaat gccttataaa attcatacat ctgcttccaa aaaaatccca
     4441 ttttaggcag gcttagtatt tttaaacaac caatccactt agatcatttt tttcagttct
     4501 aaaaacacgc tactttgcgc gcacaattag cgcaaacccc actccccacg cactttttat
     4561 gtgctcaagc acacacaaaa aatgcctctc attgcaacat tttttaacga cgcccatcac
     4621 actatcgcaa gccttatgca gcacaataaa gtgcccaggc aattgcccaa tagtcatttt
     4681 taccgcttgc accgaccatt tcaaacccga gacccgccat caattctcca gcaaaataaa
     4741 atagctaatt aactggctgt taaggccttg cggtgctttt tagaggcata aaaaaaccgc
     4801 gcccagccaa aaccaagcgc ggtttttaac accacaagac cctttagaac tgccggaaaa
     4861 acttccacga ttccgaggcg atccaggtgg ttgcaccgtt gtcgccactg cctgcatcaa
     4921 aaggcgccgc ttggtggccg ccattaaagg tacaggcttt taccggataa ttggatttac
     4981 agccctggta gtcataacac acatggcgcg aggggctaga actgctccaa gtgggaattg
     5041 tggccgccgt gcagccatta tcccgcgccc gctgctcggc aatatattta gcgccgcggc
     5101 cattacctcc atcccaaggc gtagtggtat cgctcatacc ggtggtgctc atccaggcaa
     5161 tggggtcttt aggaatgggg ctgggcagcc aaatattgta attagcaggg ccaataccca
     5221 ccgccgcgcg gatagatttt tggtggttgg tggataatga ataggtaatc atgccgccaa
     5281 aactaaaacc tgtggcaaat acccgggaca tatcgataca taaactgcca gagactttct
     5341 tcaaaatatc gtcgaataaa tagcggttat cggtttcttg ccaggtggag cccaccgatt
     5401 gcggcgcgac aaaaatagct ggatctttag cggccagaga ttcgcgacgc aggccgtaaa
     5461 aaccccaatt cgtggcgccg ccattgccgg tttcaccggt ggccacagcg gtattagtgc
     5521 tgccaatcca gtgggaggta taaaacaggc gatagggttt tgatttatcg taatccgccg
     5581 gaatatcaat aatatattcc cgtgaagcat tgttactggt gagttttaag cgggtagaag
     5641 tcgctagacc ctgcgcaact gaagtgccgc cacttaataa actcgctttg ccatcagtgc
     5701 cgcagccgct actttttact ggcgcattgc ctaggggata attacctggc tcaccctctg
     5761 tagccggtgg cggcgtgact gggtcaatgg tgcctgagta gttgccaaga taaatccaat
     5821 caatatcaac gccgtcggcg ccggtaatat tcaggcgaat tttttgcgtg ccgcctttta
     5881 aggttatttt acccattgcc agggttttaa attcgcccca ggcgcttacc tgcggcacag
     5941 caatattttc aatgagtatt ttatcgcact ggcttaaact gagggtgcgg ccggtttcca
     6001 cagcacctaa gcgcatggtg agattatatt caccttcacg ggcaacattg acggtaaatt
     6061 ccaaccactc gcccgcaacg gtccacccca ccgcataacc atcgctaatg gctttgatat
     6121 ccacccctgt gtcggtgcga taggcgccgc cctcattact cgttgtagtg tcggaatatc
     6181 ccgccggatc aaaatcttca gcttcaattt taccgggaat tagccgcacc atttgttggt
     6241 aggcgtcgct gatattacct ggacaggttg tatttatggc gccgctagat gaggtggagc
     6301 tacttgaact ggatgaggaa ctcgatgagc tggaactact agaactagtt cgaccggacg
     6361 aacttgagct tgaggagcta gaactggaac ttgagcttga tgaggacgaa ctggaactgg
     6421 cgccatccac agggccgcca caggccgaag ttactagcag cgttaaaagg cctcccgccg
     6481 ttaacacttt attgattgtt tgttgtaatt tcataaaacc tgtacctgtg gtgaagtgta
     6541 aaccattaaa aattcgtttt atttgccgtg tctttcgatg caacaggcag ttattgggca
     6601 aataatggcg cgttgattgg agacgaacat ctggttactc cgccgcacag tgacgcgcct
     6661 tttaatcgac tgactcatag gtttggctgt aaggaagacg ccacaccgga ccatttatac
     6721 cgacccgcca atggcatagg ccattaaggt catatcgtcg gattaatctt attgtcttgg
     6781 tgaatacatt caaaaagcgc tacggctttc tcaaaatggg gaaatcgctc aggcgctgca
     6841 aagtaattgc gggctagata acgccggtaa cgtgaagtga ggcgagtttt gcactatcag
     6901 cagggctgat tggccgtgaa ttgaaaaatc gactgaggaa acagcgcact tatttgcccg
     6961 ttattaacct ggcactcaat gcagcggggt taataactat tcggcacacc ttattaatcc
     7021 cctccacacc aatgtcacta agttaagaaa tgggctgcga tttggcgtca gcatatctgg
     7081 gtagcctgga tggagcgtag cgcaatccgg gatattcgag gcaccccgga ttgcgctacg
     7141 ctccatccag gctacaaaat tgggcggcaa atacaggagg agtagccatc cggcgtaatc
     7201 gattcgcggt ggtgcagtgg tgactacagg ttagtgcaga ccgtcaaaaa gagggacctt
     7261 tttgacgagc ctacagggat gtattcacgg cgtgtctgca ctaacctgta gtcaccactg
     7321 cgggtgctgg attacggggt cacagcacta aaacacacac gcgatagttc ccagtcaata
     7381 cgggaattac ctccagccac ataaaaagat gctgaatagt tacctctatt ttttatcctc
     7441 gcaaaatgga ttaccttgat caaaaataat ttcccacttg ccatcgggcc gcagccgcca
     7501 aatagaggta aatcggtggg ttattttgcc attgggatca aataccggcc cggtgctgat
     7561 ggcaagtttg cccgactcta acacttccac ctcctccggc cgccaggaaa agggagcttt
     7621 tggagcttga tacagcggct tccagtgttc cactattttg tttttcccct gcaggggaat
     7681 atgctgtaaa aaaaccgcct cttgtgcgat ataagatgta aacgccgcaa aatcgcgatc
     7741 cgccatggtt ttggcaaatg ccagctcagt tgctattact gcctctcggg cgttgtgact
     7801 cgccacttga gggttacttg cacagcctaa taatccaaac aatacagcag cccacataac
     7861 acaccggttt atattgtttt gcgctcccat acactgcccc ttttttatgc gctgcacacg
     7921 gcagccgaag aattaagaaa ataacctgta actattcagt gctgtttttt attagggaac
     7981 ctctgaaaaa agtaggcggc gaagcactag ctaggcgaat ttggcaaaaa aagcggagtt
     8041 tacacg
//
LOCUS       DAOQGS010000134         8008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4284_length_8008_cov_5.090280, whole genome shotgun sequence.
ACCESSION   DAOQGS010000134 DAOQGS010000000
VERSION     DAOQGS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_8008_cov_5.090280"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKZ52_06780"
     CDS             <1..710
                     /locus_tag="PKZ52_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNG59513.1"
                     /translation="NGTYYTPAVMGIDYALTNRFNQEEFTQANNVIEPHSDLGGLILV
                     SWTPSNPWDSGFNTGYTSQVDLPALTQGFSSEAKDRWQEDIKQLTNALLNLQQRRVAV
                     ILRPLPLMNSDRYWWGINATGANKENDFKDLWSDLFKQLNDAGIENVIWAYSPLDTSE
                     PNTKAFNWGFNKEFVDLVTPVVRNDNLDIRDYDAYKTLGKPLGLAEYSPPPAAAALTP
                     SNMKLGSLPIIRPWRFG"
     gene            707..835
                     /locus_tag="PKZ52_06785"
     CDS             707..835
                     /locus_tag="PKZ52_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59514.1"
                     /translation="MSGVNDGDIKRTLVDNKNAYDLLIKSTVINAENVDNQNLNSN"
     gene            complement(904..1206)
                     /locus_tag="PKZ52_06790"
     CDS             complement(904..1206)
                     /locus_tag="PKZ52_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59515.1"
                     /translation="MSLTASQLHSLRQCAEEECSVIEIRQELRGPGEALELAQAKLIA
                     GLTGLAEQGLVEFFLDEFAAAATRPISATEALARLNEPATWDMQNPTQLHVSWVGP"
     gene            complement(1203..1616)
                     /gene="fur"
                     /locus_tag="PKZ52_06795"
     CDS             complement(1203..1616)
                     /gene="fur"
                     /locus_tag="PKZ52_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNG59516.1"
                     /translation="MSSNQELRKAGLKVTLPRIKILQLLEQAKDRHVSAEDVYKALLE
                     AGEDVGLATVYRVLTQFEQAGLVERHNFDGGHAVFELDSGEHHDHMVCTQTGKVIEFH
                     NDQIEQLQEQIARELGYELTGHSLVLYGKPLAQPQ"
     gene            1787..2239
                     /locus_tag="PKZ52_06800"
     CDS             1787..2239
                     /locus_tag="PKZ52_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNG59517.1"
                     /translation="MPVSPRSLAFNLILICSVALGSGCSQLKFPFVYRINVQQGNIID
                     QKKLDQVKIGMNKRQVQFLLGTPLLVDTFNSERWDYIYNVRKGEKVLGEKRFTVFFVN
                     DALARYEGDVGPGKNAIADPKLDERMPTPQEQAEINKNSKSGAKKPGK"
     gene            complement(2379..3038)
                     /locus_tag="PKZ52_06805"
     CDS             complement(2379..3038)
                     /locus_tag="PKZ52_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59518.1"
                     /translation="MTVPFASWLDDSHRDQLKELVNIAMGDGAKALSAVTARYIQLSV
                     PQVTQVLTQDLISHCKFAGSREFCCCVQEYSALGQAGWLLIVINPADLKTYAALVDRD
                     CVDEADHENILVQLAGVIAPVILPRIAKELRVSLKLGQVQPYLGLSHLPALGGEGVSA
                     VNLAFSYAVEVPLESEELMGKVPVEMGFDVIMRAPADLVKQLAEKFSSRLDAPFNMGS
                     V"
     gene            complement(3049..3414)
                     /locus_tag="PKZ52_06810"
     CDS             complement(3049..3414)
                     /locus_tag="PKZ52_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG59519.1"
                     /translation="MAIPLLICDDSSMARKQVARSLPPSWEVDITFATNGIEALAAIA
                     EGKGEMMFLDLTMPEMDGFEVLENIRKKDLKSVVIVISGDIQPQARDRVMALGALDFI
                     KKPVSPEKLTETLGKFGLL"
     gene            complement(4133..5404)
                     /locus_tag="PKZ52_06815"
     CDS             complement(4133..5404)
                     /locus_tag="PKZ52_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HNG59520.1"
                     /translation="MLTTGLKQHFERTGIRWILNLWLQFFRDYRAAACQDQAAALTYM
                     TLFALVPLMTVTYAVFSMVPEFAGIGVKVQKFLFAHFVPESGAVIQAYFVNFSKQAQK
                     LTWIGALFLAVTAFLMLGNIERTFNRIWGVRRGRQGLGKVLLYWAVLSIGPLLIGSAF
                     ALSTYVVSLKYWVLGTAAIPSPFIAIYPVFLTATAFSLLFVAVPNCNVPIKYGVVGGI
                     GAALGFEVLKLLFGFVVANTNFKFIYGAFAALPLFLLWLNLLWMTVLAGAVLVRILAE
                     KRYLIMQEGYCDLASALVCLHLLRTAQRTGSAVDELDFLSAGVNPHDWQPLRDGWLGA
                     GWLSQTQSGGFVLCRDLALCTLWDLAEVINLRIADLAQAVKQLPQFPALPQLITPIFT
                     EHQKLWSVSVLPLIDQTIAQDNSAQSLHQQQ"
     gene            5463..5822
                     /gene="arsC"
                     /locus_tag="PKZ52_06820"
     CDS             5463..5822
                     /gene="arsC"
                     /locus_tag="PKZ52_06820"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467383.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNG59521.1"
                     /translation="MAAQYRIYHNPRCTKSRQTLALLQAAGITPEIILYLDTPPGTEE
                     LSLLIKQLGFASARQLLRTGEDAYKQLGLGNPAINEAHIIAAMAEHPKLIERPIVVKG
                     NKAILGRPPENVLSLMS"
     gene            5826..6428
                     /gene="wrbA"
                     /locus_tag="PKZ52_06825"
     CDS             5826..6428
                     /gene="wrbA"
                     /locus_tag="PKZ52_06825"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNG59522.1"
                     /translation="MPLPYILILYYSQNGSTRQLARALAEGVDSVKGIEARLRTVPKV
                     STECQATAPEIPLEGDIYCTEADLKNCAGLLLGSPTRFGNMAAALKYFWDGTANLWVN
                     GHLEGKPAGVFTSTSSLHGGQEATLLSMMIPLLHHGMLVAGIPYSQSALHVTSSGGTP
                     YGASHWAHGEQAAQLTTDERSLAQAQGRRLAHLALQLGNL"
     gene            6425..6799
                     /locus_tag="PKZ52_06830"
     CDS             6425..6799
                     /locus_tag="PKZ52_06830"
                     /inference="COORDINATES: protein motif:HMM:NF021369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNG59523.1"
                     /translation="MNFLTPLQLRAAQLMAVFLLILLMSFFQLALPAGPVYKIWLLQV
                     LPLLILIPGIYQLKRRSFQFMGFVILLYFIRSVTNVLSGDPAFIDYGILGLSVLIFFL
                     SLMASRVLVGNGQSPATRQEEL"
     gene            6796..>8008
                     /gene="sppA"
                     /locus_tag="PKZ52_06835"
     CDS             6796..>8008
                     /gene="sppA"
                     /locus_tag="PKZ52_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNG59524.1"
                     /translation="MIEPKASLPVRIWRGIWKVVSFIRASLANIIFLFFLIIFISAVA
                     GNRTKPIPAAAPLFIAPSGPLVDQLTYAEPISLLLGGKNARDNQTSLRELIKVIDAAA
                     SDPRITALVLQLDYLADSGSAKMAELAEAILRFKSKQKPVIAYADYYSQQQYFLAAHA
                     DEIYINDMGGVLLNGIGLYRNYYKSALDKLSVKFHIFKVGTFKDFVEPYTRNDMSEAS
                     KQHNNEWMSELWSLYTNRVETQRKLEPGSINKLINSTSEALLAANNSTAQMALNAKLV
                     DHVGNRVNRMEDLIKRFGVDPEVTSQFLHVNYNQYKHDLASKSSAETQGNIGLIVASG
                     SIVDGEQPDGTIGGDTLAQLLRKARDDKDIKALIVRIDSGGGSAFASEIIRQEMQAVR
                     AAGKKIYVSMGT"
BASE COUNT         2107 a         2191 c         1877 g         1833 t
ORIGIN      
        1 acaatggcac ctactacacc cccgcagtga tgggcattga ttacgccctc accaatcgtt
       61 tcaaccagga ggaattcacc caggccaata atgttattga gcctcactcc gacctaggcg
      121 gtttgatcct cgtcagttgg acaccttcta acccctggga tagcggtttt aataccggtt
      181 acacctccca ggtggacttg cccgcactga cgcaaggttt ttccagcgag gccaaagacc
      241 gctggcagga agacatcaag cagctgacta acgccctgtt aaacctgcag cagcgcaggg
      301 ttgccgttat cctgcggccg ctgccgctaa tgaattccga ccgctattgg tggggcatta
      361 acgccacagg cgccaataaa gaaaacgact ttaaggatct ctggagcgat ctgtttaaac
      421 agctcaatga tgcgggcatc gaaaacgtga tttgggccta ctcaccctta gatacctcag
      481 agcccaacac caaagccttt aattggggct ttaataagga gtttgtggac ctggtaactc
      541 cggtggtgcg caacgataat ttggatatcc gcgactacga tgcctacaaa accctcggca
      601 aacccctcgg cctggccgag tacagcccgc ctccggcagc ggcagctttg acaccctcaa
      661 atatgaaact cggctcatta ccaattatcc ggccatggcg ttttggatga gcggggtgaa
      721 tgatggggac atcaaacgca ccctggtgga taacaaaaac gcttacgact tgctgatcaa
      781 atccaccgta attaatgccg aaaacgtgga caaccaaaac ctcaacagta attaacgccc
      841 cctctgggtg ccctcaaggg gggcacctgt taacacaggc aagattttgg ttttcggttg
      901 tgcctatggg ccaacccagc tcacatgcaa ttgagtaggg ttttgcatat cccaggttgc
      961 aggctcgttt aggcgggcga gcgcctcagt ggcgctgatg gggcgggtgg cggcagcggc
     1021 aaattcatcg aggaaaaact caaccagccc ctgttcggca aggccagtaa ggcctgcgat
     1081 gagctttgcc tgggccagct ccagcgcctc gcccggcccg cgcagctcct gccgtatttc
     1141 aatcacactg cattcctcct cggcgcattg ccgcaggctg tgcagttggc tggcagttaa
     1201 actcattgcg gctgggccag aggtttgccg tacagcacca ggctgtggcc ggttaactca
     1261 taacccagct cgcgggcaat ttgctcttgc aactgctcaa tttgatcgtt gtgaaactca
     1321 atgaccttgc cggtttgggt gcataccata tggtcgtggt gctcgccact gtccagctca
     1381 aacactgcgt ggccgccatc aaaattgtgg cgctccacca aacccgcctg ttcaaactgt
     1441 gtgagcaccc ggtacacagt ggccagcccc acatcttccc ccgcttccaa tagggccttg
     1501 tacacatctt ccgcgctgac atggcggtct ttggcctgct ccaacaactg taaaatttta
     1561 atgcgtggaa gcgtgacttt tagccccgct ttgcgaagct cttggttgct gctcatggga
     1621 attctcgttg tgaccggttg gggaacttgc aggccagcgc aagcgtctgg agcgagcccg
     1681 gattttacct gcgatggcgc gcacctacta gcggttgttt gccttacccc taaactcagg
     1741 tatcatccgg cgccgttttt gccatcccat tcactgagat atgtccatgc cagtatcgcc
     1801 acgaagcctc gcgttcaacc ttattcttat ttgtagcgtg gcgttaggta gcggctgctc
     1861 gcagctgaaa ttccccttcg tgtaccgcat taacgtgcaa cagggcaata tcatcgacca
     1921 gaaaaaactc gaccaagtca aaatcggcat gaacaaacgc caagtgcagt ttttactggg
     1981 caccccactg ctggtggata cctttaacag cgagcgctgg gattatattt acaacgtgcg
     2041 caaaggcgag aaggtgcttg gggaaaagcg ctttacggtg ttttttgtga acgatgccct
     2101 ggcccgctat gaaggcgacg tgggccccgg caaaaacgct attgccgatc ctaaactcga
     2161 tgagcgtatg cccaccccgc aggagcaagc ggagattaat aaaaattcca aatctggcgc
     2221 taaaaagccg ggcaaataaa aagaattttt taaacgagga caccgctagg cttaaggcct
     2281 agcgcgcata gagagcagta gcaacaaaac gagacaagtg acaagtagtc accaaaaccg
     2341 agatgtgagc ggcgaaacac tcactgcccg cccctagctc aaacgctacc catattaaac
     2401 ggcgcatcca aacggctgga gaatttttcc gccagttgtt ttaccaggtc cgccggggcg
     2461 cgcatgatga catcaaagcc catttccacc ggcaccttgc ccatcaactc ctcagactcc
     2521 agcggcactt ccaccgcata agaaaaggcc aaattaaccg cacttacgcc ctcgcccccc
     2581 agcgcaggca agtgggataa acccaaatag ggctgcacct ggccaagttt taaggaaacc
     2641 cgcagctctt tagctatgcg cggcagaatg accggggcaa taacccccgc cagctgcact
     2701 aatatatttt cgtgatccgc ctcgtccaca caatcgcgat ctaccaaggc ggcataggtt
     2761 tttagatcgg caggattaat cacaatcaac agccagcctg cctgccccaa agcgctgtac
     2821 tcctgcacac aacagcaaaa ctcgcggctg cctgcaaatt tgcagtggct gatcaaatcc
     2881 tgggttaaca cctgtgtcac ctggggcaca ctcagctgga tatagcgcgc ggtgacggcg
     2941 cttaacgcct tagcaccgtc gcccatggca atattaacca gctcttttaa ttgatcgcgg
     3001 tgtgaatcat ctagccaact ggcaaatggt actgtcataa cgcaccccct aaagcaggcc
     3061 aaatttgcct agtgtttcag ttaatttctc tgggctgacc ggctttttaa taaagtccaa
     3121 cgcacccaac gccattaccc ggtcccgcgc ctggggctga atatcgcccg agataacaat
     3181 caccacactt tttaaatctt ttttgcgaat attttccaac acctcaaagc cgtccatttc
     3241 aggcatggtc aaatccaaaa acatcatctc acccttgcct tctgcaatgg cggcaagcgc
     3301 ctcaatacca ttggtggcaa aggtgatatc cacttcccag ctcggcggca gcgagcgcgc
     3361 cacctgttta cgggccatac tggagtcatc gcaaattaat aggggtattg ccataggcca
     3421 atactcttgt gttggttaca aggctgagta tagacagagt ttgctaaagc gagggttgaa
     3481 agccgcttga ttaacctaga aactgcgcca aaaattgttt cgccgcaaac tgacgggcag
     3541 gccgatttag gcgagggtta agaaaatcgg caaaaaaatg caggatatta tgcagcatgc
     3601 aagccgggat gctcgctgta cgcacggcag cagtacacct agttgagcgc ttggtgaaat
     3661 agcagctagc accagacagt gtattcactc actgcagaca gatattaacc cggcggcatt
     3721 tagtgccggg ttaataatgg cggcatggat gccgcgccaa aggtgcgagc cgccgcgagc
     3781 tgcactaaaa caagaaaatc cgcgcatttt cgtaagttta gtgcagcgaa ggcaaaatag
     3841 atcaggacga tctattttgc cgccaggtta ataggaatta aacgccagcg tgaatcatat
     3901 ttgacgcttt atcaaatcga ttaaaccata aaatatggat tgtcaaatta atggcgaaca
     3961 attcaataat aaatgcaact attcagacca ttttgttgta gatggagctg attctcgtgc
     4021 cgaatagaaa tcaccgggcg aatacttttg tgttgtgagc ccccagcact cccccccagt
     4081 gatggctcgt tactggtaaa cagcgtatgg caatcaagcc caaagccgca actcactgct
     4141 gctggtgtag gctctgcgcg ctattatcct gcgcaatggt ttgatcaatc aggggcaaaa
     4201 ctgacacact ccagagcttt tgatgctcag taaaaatggg cgtaatcaac tgcggcagag
     4261 cgggaaattg cggcaattgt ttaaccgcct gggctaaatc cgcaatccgc agattaatca
     4321 cttcggctaa gtcccataaa gtacacaacg ccaaatcacg gcacaacaca aaaccgccgg
     4381 actgggtttg gctcagccag ccagcaccca gccagccatc gcgcagcggc tgccagtcgt
     4441 ggggatttac gcctgcggat aaaaaatcca gctcatccac ggcgctgccc gtgcgctggg
     4501 ccgtgcgcag cagatgtaaa cacaccagtg ccgaggctaa atcacaatag ccctcttgca
     4561 taattaaata gcgtttttcc gctaaaatgc gcactaaaac tgccccggct aacaccgtca
     4621 tccagagcag attgagccaa agtaaaaaca agggcaatgc ggcaaaagcg ccataaataa
     4681 atttaaaatt ggtattggcc accacaaaac caaataataa tttcaacacc tcaaacccta
     4741 aggcggcgcc tatgccacct accacaccgt atttaatcgg tacgttgcaa ttgggtacgg
     4801 caacaaataa aaggctaaat gctgtagccg ttaaaaatac cggatatata gcaataaacg
     4861 gcgatggaat agccgctgta cccaataccc agtattttaa tgacaccaca taggtagaaa
     4921 gcgcaaacgc gctgccgatc aacagcggcc caatacttaa taccgcccaa tataacagca
     4981 ctttgcccag cccctggcgg ccgcgccgca caccccaaat tcgattaaac gtgcgctcaa
     5041 tattgcccag catcaaaaac gcggtaaccg ctaaaaacag cgcaccaatc caggtgagtt
     5101 tttgcgcttg tttggagaaa ttaacaaaat aggcttgaat aacggcgccg gactccggca
     5161 caaaatgggc aaataaaaac ttttgcacct tcacacctat gcctgcaaat tccggcacca
     5221 tggaaaatac tgcataggtc acggtcatta agggcactaa ggcaaaaagg gtcatatagg
     5281 tgagcgcggc cgcctgatcc tggcaggcag cggcgcgata atcgcgaaaa aattgcagcc
     5341 aaagattcag tatccagcgt atacccgtgc gctcaaaatg ctgttttaaa cccgttgtta
     5401 gcatgttggg tcaaactccc ggcagtggta cactgcaaaa actcaaactc aattgaatga
     5461 ctatggctgc tcaataccgt atttaccaca acccgcgctg tacaaaatcc cgccaaactc
     5521 tagccctttt acaggcggcg ggcatcacac ctgaaataat actctacctt gatacccctc
     5581 ctggcaccga agaactaagc ctgctgataa aacaactggg ttttgcttcc gcgcgtcaat
     5641 tactgcgcac cggtgaagac gcctataaac aattagggct tggcaatccc gccatcaatg
     5701 aggcacacat cattgcagcc atggcagagc accctaaact aatagagcgc ccgatagtag
     5761 ttaagggtaa caaagccatc ctcggtcgcc caccggaaaa tgtcctcagc ttaatgagtt
     5821 aattcatgcc gctcccctac attttaattt tgtattacag ccaaaatggc agtacgcggc
     5881 agcttgcccg cgccctggcc gagggcgtcg attcagtaaa gggtattgaa gctcgcctgc
     5941 gcacagtgcc taaagtctca acagaatgcc aagccactgc cccggaaata ccgcttgaag
     6001 gggatattta ctgcactgag gcggatttaa aaaactgcgc cggtttatta ctcggcagcc
     6061 ccacacgctt tggcaatatg gccgccgccc taaaatattt ctgggatggc accgccaatc
     6121 tgtgggtaaa tggccattta gaaggtaaac ccgccggtgt ttttacctcc accagctccc
     6181 tgcacggcgg gcaggaagca acactattaa gcatgatgat tcccctgctc caccacggca
     6241 tgctggttgc cggtatcccc tattcccaat ccgctttgca tgttaccagc agcggtggca
     6301 cgccctacgg cgcttcacac tgggcccatg gcgagcaagc ggctcaattg accactgatg
     6361 aacgctcact tgctcaagcc cagggccgcc gtttggccca cttagcacta caactaggaa
     6421 acctatgaat tttttaactc ccttacagtt gcgcgccgcg caattgatgg ctgtttttct
     6481 cctcatttta ttaatgagtt tttttcaact cgcgttaccc gccgggcctg tttataaaat
     6541 atggctgctg caggtgctgc cgctgttaat tttaattccc ggcatctacc agctgaagcg
     6601 gcgcagtttc caatttatgg gctttgtgat tttgctgtat tttattcgct cggttaccaa
     6661 tgtcctctcc ggggatccag cctttattga ctacggcatt ctgggcttga gcgtattaat
     6721 tttcttttta agcctgatgg ccagccgcgt tttagtcggc aacggccaat cgcccgccac
     6781 acgacaggaa gaattatgat tgaacctaag gcatctttgc ccgtgcgtat ttggcgtggc
     6841 atatggaagg tagttagttt tattcgggcc agcctcgcca atattatctt tttatttttt
     6901 ctgattattt ttatcagtgc tgttgcgggc aaccgcacta aacctattcc cgccgcagcg
     6961 ccgttattta ttgccccctc tgggccgctg gtggatcaac ttacctacgc agaacccatc
     7021 agcctattat taggcggcaa aaacgcccgc gataatcaaa ccagcttaag ggaacttatt
     7081 aaggttatcg atgctgccgc cagcgaccca cgcattaccg cattggtact gcaactcgat
     7141 tatctggccg atagcggcag tgccaaaatg gccgaattag ccgaagccat cctgcgcttt
     7201 aaatccaagc aaaagccggt tattgcgtac gctgattatt actcccagca gcagtatttt
     7261 ctcgccgccc atgccgacga aatttatatt aacgacatgg gcggtgtatt actcaatggc
     7321 attggcctat accgcaatta ctacaaatct gccctggata agttgtcagt aaaatttcat
     7381 attttcaaag tggggacatt taaagacttt gtagagccct atacccgcaa tgatatgtcg
     7441 gaggcctcca aacaacataa taatgagtgg atgagcgagc tttggagcct ctacaccaat
     7501 cgggtagaaa cccagcgcaa actggagccc ggcagcatta acaaactgat taattccacc
     7561 agtgaagcct tattagcagc gaataattcc accgcacaaa tggccctaaa tgccaaactc
     7621 gttgaccatg taggcaaccg cgtaaaccgc atggaggatt taattaaacg ctttggtgtt
     7681 gaccctgaag ttacatctca gtttttgcat gttaattaca accaatataa acacgacctc
     7741 gccagtaaaa gcagcgccga aacccaaggc aatattggct tgattgtagc cagcggctct
     7801 attgttgatg gcgaacaacc cgatggcacc atcggcggtg acaccctcgc gcagttattg
     7861 cgtaaagccc gcgacgataa agacatcaag gccttaattg tgcgcataga ttccggcggc
     7921 ggcagtgctt ttgcgtcgga aattatccgt caagaaatgc aagcggtgcg tgcggcgggg
     7981 aaaaagattt atgtatccat gggtacag
//
LOCUS       DAOQGS010000135         7995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4296_length_7995_cov_4.500882, whole genome shotgun sequence.
ACCESSION   DAOQGS010000135 DAOQGS010000000
VERSION     DAOQGS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_7995_cov_4.500882"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKZ52_06840"
     CDS             complement(<1..299)
                     /locus_tag="PKZ52_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG59525.1"
                     /translation="MKQNMYIKNQQAGFTLVEIAIVLVIIGLLLGGVLKGTELIENSK
                     VKRAVNELNSITAALYAYQDRYQRLPGDDGPNLATLTGRGGPWTAVTVAGNNNGV"
     gene            complement(1138..1827)
                     /locus_tag="PKZ52_06845"
     CDS             complement(1138..1827)
                     /locus_tag="PKZ52_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNG59526.1"
                     /translation="MMLSSSSNNRGFTLIEMAIVMVILGALIGGLLVPLASQREVNQR
                     KAAAQLLQELRQALLGYAVIHGALPCPASATSNGAEAPRTGANGCSLANGFVPYALLG
                     MQASIKNGVLVDPWQGNVRYSLSPANNWVYAQSIPLTSAVATTLRVCDSSPCTAANTL
                     AANLAAVLVSTGPDGNNQPASTSPDQVANAAGGPSFISREPSALSGSEFDDQLVWLAQ
                     ASLVYELSKVK"
     gene            complement(1802..2932)
                     /locus_tag="PKZ52_06850"
     CDS             complement(1802..2932)
                     /locus_tag="PKZ52_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59527.1"
                     /translation="MALLAVLGLLLLIFTGVLIGRLSLNDQARQRQQVTAASLLAVRD
                     ALVGYSLVAASNSQPPGSLPCPAQNRAGQPAVNGATGACLQLQGLVPYRALGLSEPLD
                     ATGTPLWYAPAAALTGNTNPPLSLRNSSTLSSLTLKLNAANRVQAVAFVLLAANAPLA
                     GQQPVSAPLAAASQFLEGANGVNNATAYDDLRDADHNDQVLGMPLGQFWSSVEQRVLT
                     EVQQTLQLYRLRCGAYPWAAPWGVAGYNSQANLASGALPVGTAVPVNWAASCGANQAP
                     ALAPWLRNHWGGLLHYALCDQPAGNPPSASCLQLTTAAGTAQAKAVVVAPGTLLNQQN
                     RSLTALNHFFEQANADANPRSFTQLPPGQHTQGFNDVVFIVE"
     gene            complement(2958..3644)
                     /locus_tag="PKZ52_06855"
     CDS             complement(2958..3644)
                     /locus_tag="PKZ52_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59528.1"
                     /translation="MSGRTHRIWPAPWRLAQAVCGRVLACCYFLACAWALGAEQAAAQ
                     SGQVASAQGLHTAGPDDLPFARLFNTPDQRQSADRRRARQNTPGAAVTPSAPGPAAAG
                     QPMRLSGVMVRADGEQVLWVNGQLQRQSAASPRFKVLGQQRSDSLRVHMAARELKPGQ
                     VWRSGSGKAQEAYQVAPPRPAATPGQGDDKKAAEQATHAIDQTASEPLPTTPAHSAPL
                     PAAQERSPER"
     gene            complement(3753..4448)
                     /locus_tag="PKZ52_06860"
     CDS             complement(3753..4448)
                     /locus_tag="PKZ52_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59529.1"
                     /translation="MTESVHPATAAPTQLDRPVKSLHPGLVFIRQHWLTSLALALSLL
                     VLALALGRLQQARGAFAQEQARYRQQLSSHQSAAANANLLAAYQAPYAAFISRGHIGA
                     AQRLQWVEALSWGVNHLAVPQVRFTLEPTLPATLDKHPAGQVEGLSLTPMRLDFTLLH
                     EGDFYRLLQDLRSRALGLFSVPSCTLRRKDAGSIESSEARAQNPGEASRIVGECELLW
                     YNLQPIADPPPDS"
     gene            complement(4445..6301)
                     /locus_tag="PKZ52_06865"
     CDS             complement(4445..6301)
                     /locus_tag="PKZ52_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59530.1"
                     /translation="MARRVFYFSGYRLKVFEWQGKQLLGSAVFEPTAAGFADFAAYLS
                     QAPVLPAQLLVDVIEEDFRRESIPHLGPKDRQALLARLLDRLHRGEPYVHVLPLGRSR
                     EGRRDDVLLVSALTNSELLSPWLAPLLEREIPLAGIWSLPLLSAKLLPACHANEDNVL
                     LLSQQIRTAHRETYFNKGQLLFSRQAKLERGLRDAFEVAHRAQAISKSSEQIRIFLTN
                     QRTMGFADKLHVYCLLPEDSVAPLSALTPDSAGISYHPVPLGRLYAHFKLPQEIVPKE
                     DVLFSYLCASAPLAPDHYATPVQKKFFRRLRLDKYLARAGVLACVLALAAAGVLWLYS
                     LDLDQQRQQLITQRQVLERRYQLDFAPRQAALDQAASVQNAVELMRRLEQEALQSPDQ
                     LMAPLSYVFSDTRFAVLQLDSLSWKKYPPGALAQLVAQTQASVSAASATADSQEQAQP
                     EESAEALQASAIVAGYLRRGDMSYRQTVALMTQFAEALRQRPEIAAAVTVKMPVDVRP
                     PLTFVDDSGADRGAVNPQQEQENNRFEILLALALPAPLVPLDTPAQPGDASTEAVDAS
                     SEAVDASGEAVDTRSEDPNARPLTSAAGAADGGDLAQQNHATAASLPIKASP"
     gene            complement(6399..7601)
                     /locus_tag="PKZ52_06870"
     CDS             complement(6399..7601)
                     /locus_tag="PKZ52_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNG59531.1"
                     /translation="MGQFNYKSIDKNGLLSKGRMQASNPLDLEHRLAQQGQELITCAE
                     IKPGGLFRGRHKLTRRDLINMLFQLEQLTKSGVPLLEGLVDLRDSTSPGYYRDVLAGL
                     VESIEGGKTFSAALADFPQDFDTVFISLIAVGEETGELPKILKDMGETLRWTDELIAA
                     TVKIIIYPAIVALVVFAVAAFLMIYLVPKIIPFVKEMGAQIPVHTQALIAVSDFFIHY
                     WWLLMLTPVALIVLVKSAAKKSPELRFKIDGLKLRVPIFGAISFKIKLARFASYMALM
                     YASGITVLRALEICQALVDNAVMANSLQRAGRHIAEGRSISESFARLGLFPPLVVRML
                     KVGEQTGNLDEALLNVSYFYNREVQEAIATIEPAISPILTVVMGTLLGWIMLSVLGPV
                     WDAVSKAG"
     gene            complement(7604..>7995)
                     /locus_tag="PKZ52_06875"
     CDS             complement(7604..>7995)
                     /locus_tag="PKZ52_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HNG59532.1"
                     /translation="DIMAGNIIGIIGQRPVRTLCSFCKHPRRAEDVERLILGVDGPVT
                     VWDACGCERCGGTGYKGRVSVLEILKMDAELDELITQRASLRVMLRAAVGKGFKTMAD
                     DGIRRVLEGHTTLEELSRVVDLTGRLA"
BASE COUNT         1654 a         2381 c         2360 g         1600 t
ORIGIN      
        1 atgacgccgt tgttattgcc cgccactgtg accgccgtcc aagggccgcc ccgccccgtg
       61 agggtggcca ggttcgggcc gtcgtcaccg ggtaggcgct gatagcgatc ttggtaggcg
      121 tatagcgctg cggtaatgct gtttaattca ttcactgcgc gttttacttt ggaattctca
      181 attaactctg tgccttttag taccccgccg agcaataacc cgataatgac caacacaatg
      241 gcaatttcaa ccagggtaaa acccgcttgt tggtttttga tatacatatt ttgtttcata
      301 ttgacttcct ttgattaaat caaccttcag tagctacaaa atgcaaccca aggcactttt
      361 ttgagtgtag caacgattct taaaaaaata ttttaagcgc acagcttcca aacccgggac
      421 caccttaaaa agcaaaaaat acttacaaaa tcagaatttt taaatatgct cagttaattc
      481 gacacaccca aatattcaca ccgatatacg tgttttattt ttgacgcccg tgattatcta
      541 taaaattttc acctggcaaa ttcaaatttt tgacaccagc gttgataaaa actcagccat
      601 taccggcggg ttatgtgtaa ccagcaagta ctttcgtgct gcaatctctc aacctcaaaa
      661 aaatgccttg agctaaggat ttttggcgcc tgcgcgccca aaaattctcc tcccgtggag
      721 ggtaatatca ttgagtaaag cgttttgagg tgaattttta caataggttg gaagagcgca
      781 gcgacttccg acatcctgcg gagtccttgc cgtgcctgga ctttattctt gcggagggat
      841 gtcggaagtc gctgcgctct tccaacctac gcaataatgg gcttttcctt aacttaaaag
      901 gcattaccct ggggggcagg cctgcctgcc ggggtggttg cggtggatag cccggttgta
      961 cttcaggagg cttttatttc gagtgacttt ttagttcgag tgaacaggaa atcttgaatt
     1021 acagcgggct gctacggcgt tttttgggat tatgcaggtt ggctaaatcc tccacatttt
     1081 gccgcccgcg cagaatagag cgattaagag agcaataagg cgtgcagtag tcacggctta
     1141 tttcactttg ctcagttcgt acaccaggct ggcctgggcc aaccagacca gttgatcgtc
     1201 gaattcgctg ccgctgaggg cgctgggctc gcggctgata aaactgggcc cacccgctgc
     1261 attggcaacc tggtcggggg aggtagaggc gggctggttg ttgccatcgg ggccggtgga
     1321 taccagcacg gcggcaaggt ttgccgccag cgtattggca gcggtacagg ggctgctgtc
     1381 acagacgcgc aatgtggtgg ccacagcaga ggttaggggg atgctctggg cgtacaccca
     1441 attgttggcc ggcgagaggc tgtagcgcac attgccctgc caagggtcca ccaacacacc
     1501 atttttgatg ctggcctgca tgcccaacag agcatagggc acaaaaccat tagccaggct
     1561 gcagccattg gcgcccgtgc gcggcgcctc agcaccgttg ctggtggcgg aggccgggca
     1621 cggcagtgcg ccgtgaatca ccgcataacc cagcagcgct tggcgcagct cctgcaacaa
     1681 ctgagcggca gctttgcgct ggttcacctc cctttggctg gccaagggca ccagcaggcc
     1741 gcctatcaga gcgcccagaa tgaccatcac aatagccatc tcgataagcg taaaacctcg
     1801 gttattcgac gatgaagaca acatcattaa aaccctgggt gtgttggccc ggcggcagct
     1861 gggtgaaact gcggggattg gcatcggcgt tggcctgttc aaaaaaatgg ttcaacgcgg
     1921 taaggctgcg gttttgctgg ttgagcaaag tgcccggtgc gaccactaca gccttcgcct
     1981 gggcagtacc ggcggctgtg gtcagctgta aacaactggc ggagggtgga ttgcccgccg
     2041 gttgatcgca cagagcgtaa tgaagaagcc caccccaatg gttgcgcagc caaggggcga
     2101 gggccggcgc ctggttagcg ccgcaactgg ccgcccaatt gaccggtacg gcggtcccca
     2161 caggcagggc gccgctggcc aaattggctt ggctgttgta gcccgccacg ccccagggcg
     2221 ccgcccaagg gtaagcgccg cagcgcaggc ggtagagctg cagggtttgt tgcacttccg
     2281 tcaacacgcg ctgttcgaca ctggaccaaa actggcctag gggcataccc aacacctggt
     2341 cgttgtggtc ggcatcgcgc agatcgtcat aggcggtggc gttatttacc ccattggccc
     2401 cctccaaaaa ttggctcgct gccgccaggg gtgcgctaac cggttgctgc ccggccaggg
     2461 gtgcattggc cgccagcagc acaaaggcca cggcttgcac gcggttggcg gcattgagtt
     2521 ttagcgtcag tgaactgagg gtggaactgt tgcgcaggga caggggtggg ttggtattgc
     2581 ccgtgagggc cgccgcgggt gcataccaca gcggcgtacc cgtagcgtcc aacggctcac
     2641 taaggcccag cgcccgatag ggcaccaagc cttgtagttg taagcaagcg ccggtggcgc
     2701 cattcaccgc cggctggccg gcgcgattct gggctgggca cggcaggctg cccggcggct
     2761 ggctgttgga ggccgccacc aggctgtacc ccaccagggc gtcgcgcacg gcaagcaggc
     2821 tcgcggcggt cacctgctgg cgctggcggg cctgatcatt gagcgatagg cggccgatca
     2881 atacgccggt aaaaatcagc agcaacaatc cgagcacggc caggagcgcc atgccacgct
     2941 cgaactcggg ggtcgcttta gcgctcaggt gagcgctcct gcgccgcagg cagcggtgcc
     3001 gaatgggccg gggtggtcgg caatggctcg ctagcggtct ggtctatcgc atgcgtcgcc
     3061 tgctcagctg cctttttatc atccccctgc ccaggcgtgg cggctgggcg cggtggcgca
     3121 acctgatagg cctcctgcgc cttgccgctg ccgcttcgcc acacctggcc gggcttaagc
     3181 tcgcgggcag ccatgtgcac ccgcagggaa tctgagcgct gctgccccag caccttaaaa
     3241 cggggcgagg ctgccgattg ccgctgtaat tggccgttga cccagagcac ctgctcgcca
     3301 tcggcgcgca ccatcacccc ggacaagcgc ataggctggc cagccgccgc aggcccaggc
     3361 gcgctggggg taactgcggc gccgggtgtg ttctggcggg cgcggcggcg gtcggcgctt
     3421 tggcgctggt ccggcgtatt aaacaggcgc gcgaacggca agtcgtctgg ccctgcagtg
     3481 tgcaaccctt gggcgctggc cacctggcca ctctgcgccg ctgcctgctc tgcgcccaag
     3541 gcccaggcgc aggcgaggaa atagcagcac gccaataccc ggccacagac tgcctgggcc
     3601 aggcgccatg gcgcaggcca aatcctatgg gtgcggccac tcatcgttta actccactgc
     3661 tggttgtatc agggcgatca cctcaggtag ctgtatgtag gtaaatatgt ataggtaaat
     3721 atgcatagct aactatagct agttgaacag ggttaagaat ccgggggtgg atcggctatg
     3781 ggctgcaggt tgtaccagag cagctcacac tcaccgacaa tcctgctcgc ctcgcctgga
     3841 ttctgcgcgc gggcttcgga cgactctatg ctaccggcgt ctttgcgccg caaagtgcag
     3901 cttggcacgc tgaacaaacc gagcgcgcgg cttcttagat cctgcagcag gcggtaaaaa
     3961 tccccctcgt gtagcaacgt aaaatccagg cgcatggggg ttaggctgag cccttcaacc
     4021 tgccctgccg ggtgtttgtc cagcgtggcc ggcaaggtgg gttcgagggt aaaacgcacc
     4081 tggggtaccg ctaggtgatt caccccccag ctgagtgctt ccacccactg taagcgctgg
     4141 gcggcaccta tgtgcccgcg gctaataaac gccgcatagg gcgcctgata agccgcaagc
     4201 agattggcat tggcagcggc gctctggtgg ctgctcaact gctggcgata gcgggcttgc
     4261 tcctgcgcaa acgcgccccg cgcctgctgc aaacgcccca gcgcaagggc cagcaccaac
     4321 aacgacaggg cgagcgccag gctcgtcagc caatgctggc ggataaagac caggcctggg
     4381 tgcaggctct taacgggccg atcaagctgt gtcggcgcag ccgttgctgg gtgtacagat
     4441 tcagtcatgg cgacgccttg ataggcagag aagcggctgt ggcatggttt tgctgcgcca
     4501 ggtcgccgcc gtccgccgcc cctgcagcgg aggttagggg cctggcgttt ggatcctcgc
     4561 tacgggtatc gaccgcctcg ccactggcgt caaccgcttc actactggca tcaaccgcct
     4621 cggtactggc atcgccaggc tgggcaggtg tatccaatgg caccagcggc gcgggcagcg
     4681 ccagggctaa gaggatttca aagcgattgt tctcctgttc ctgctgcggg ttcacagctc
     4741 ccctatccgc gccggaatcg tccacgaagg tgagcggcgg gcgcacgtcc accggcattt
     4801 tgacggtgac ggccgcagcg atttcgggcc gctgccgcag ggcctcggca aactgcgtca
     4861 tcagcgccac cgtctggcgg taactcatat caccccggcg caagtagccg gccacaattg
     4921 ccgaagcttg caaggcctcg gcagattcct caggctgggc ctgctcttgg ctgtcagccg
     4981 tcgcactggc agcgctgaca ctggcctggg tttgcgccac caactgcgca agcgcgccgg
     5041 gggggtattt tttccagctg aggctatcca actgcaagac cgcaaagcgc gtgtcgctga
     5101 acacatacga caggggcgcc attaattgat ccgggctctg cagtgcttcc tgctctaggc
     5161 ggcgcattaa ctcaaccgcg ttttgcacac tggcggcctg gtcgagggcg gcctggcggg
     5221 gggcaaaatc gagctgatag cgccgctcca gcacctggcg ctgggtgatc aactgctggc
     5281 gctgctgatc cagatcaagg ctgtacagcc agagcacacc cgctgcggcc agtgccagca
     5341 cgcaggcgag cacaccggca cgggccaggt atttatccag gcgcaaacgg cggaagaact
     5401 ttttctgcac cggggtggcg taatggtccg gcgccagggg ggctgaggcg cagaggtaac
     5461 taaacagcac atcctctttt ggcactatct cctggggtag tttgaagtgg gcatagagcc
     5521 tgcccaaggg cacagggtgg tagctgatac cggcggaatc cggtgtcagc gctgacagcg
     5581 gcgcgacact atcttcgggc agcagacaat acacatggag tttatcggcg aaccccatgg
     5641 tgcgctgatt ggtgagaaag atgcggatct gctcgctgct tttgctaatc gcctgggcgc
     5701 gatgggcaac ctcaaaggcg tcccgcaggc cccgctctaa tttcgcctgg cgggagaaca
     5761 acagctggcc cttattaaaa taggtctccc ggtgggcggt gcgaatctgc tgcgacagca
     5821 gcaaaacatt gtcctcattg gcatgacagg cgggaagtaa tttggcgctc aacagcggca
     5881 gggaccagat gcccgccagg gggatctccc gctccaacag cggcgcaagc caagggctga
     5941 gcagttcgct attggtcagc gccgacacca gcagcacatc atcccgccgc ccctcgcggc
     6001 tgcgccccag gggtaacaca tgtacatagg gttccccccg gtgcaagcga tcgagcagcc
     6061 gcgccagtag cgcctgacga tccttagggc ccagatgggg gatggattcg cggcgaaaat
     6121 cctcttcgat gacatccacc agcagctgcg cgggcagcac cggtgcctgg gacaaataag
     6181 cggcaaaatc ggcaaagccc gccgcggtcg gctcaaacac ggcactcccc agcagctgtt
     6241 tgccctgcca ctcaaaaacc ttcaggcgat agccggaaaa ataaaaaacc cgtctagcca
     6301 tcagcccgcc ttgctcacgg cgtgacatgc tgcaccgagc cgcgatttct caaactgcgg
     6361 gtacaaccag gtttcttgat tcgcctctcg cctgtccatc agcccgcctt gctcacggcg
     6421 tcccacaccg ggccgagcac cgacaacata atccaaccca acaaagtgcc cattactacg
     6481 gtgagaatgg ggcttatggc gggctcaata gtggcgatgg cctcctggac ttcgcggttg
     6541 tagaaataac tgacattgag cagggcctcg tctaggttcc ccgtctgctc ccccaccttg
     6601 agcatgcgca ccaccagggg cgggaacagg cccaagcggg caaaactctc gctgatgctg
     6661 cgcccttcgg cgatatggcg gccagctcgc tgcagggaat tggccatcac cgcgttatcc
     6721 accagggcct ggcaaatttc cagggcgcgc aaaaccgtga tgccggaggc gtacatcaag
     6781 gccatataac tggcaaagcg ggccagtttt attttgaaac taatggcgcc gaaaatcggt
     6841 acacgcaatt taagcccatc gattttaaag cgcagttcag gggatttttt cgctgcggat
     6901 ttcaccaaca ctatcagggc cactggggtg agcatcaaaa gccaccagta gtgaataaaa
     6961 aaatctgaaa cggcaatcag cgcttgtgtg tgcacgggaa tttgcgcacc catttctttc
     7021 acgaagggaa taattttcgg caccaggtaa atcattaaaa atgccgccac ggcaaatacc
     7081 accagcgcca ctatggccgg gtaaataatg atttttaccg tggcggcaat taattcatcc
     7141 gtccagcgca gggtttcgcc catgtcttta agtattttcg gcagctcccc ggtctcctcg
     7201 ccgacggcga tcagggagat aaacaccgta tcaaaatcct gcgggaaatc cgccagcgct
     7261 gccgaaaagg ttttaccgcc ctcaatggat tccaccaggc ctgccaacac atcgcggtaa
     7321 taacccgggc tggtgctatc gcgcaaatcc accagcccct ccaaaagcgg cacaccggat
     7381 ttggtcaact gttccagctg gaacagcata ttgatcaggt cgcggcgggt aagcttatgg
     7441 cggccgcgaa acaggccgcc gggttttatt tcagcgcagg taattaattc ctgcccctgc
     7501 tgggccaggc ggtgttctaa atccagcggg ttgctcgcct gcatacggcc cttgctgagc
     7561 aggccgtttt tgtcgatgga tttgtaatta aactgcccca taactatgcc aagcgcccgg
     7621 ttaaatccac cacccggctg agctcttcga gggtggtatg cccctccagc acccggcgga
     7681 taccgtcatc ggccatggtt ttaaagccct tgcccaccgc cgctctgagc atcacccgca
     7741 ggctggcccg ctgggtaatc aactcatcaa gctccgcgtc catttttaag atttccaaca
     7801 ccgaaacccg ccctttgtag ccggtgccgc cgcagcgctc acagccgcag gcatcccaga
     7861 cggtgaccgg cccatccacg cctaaaatca gccgttccac gtcttcggcg cggcgcggat
     7921 gcttgcagaa cgaacacagg gtgcgcaccg ggcgctggcc gataatgccg atgatattgc
     7981 ccgccataat gtccg
//
LOCUS       DAOQGS010000136         7993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4298_length_7993_cov_4.036785, whole genome shotgun sequence.
ACCESSION   DAOQGS010000136 DAOQGS010000000
VERSION     DAOQGS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_7993_cov_4.036785"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..2030)
                     /locus_tag="PKZ52_06880"
     CDS             complement(180..2030)
                     /locus_tag="PKZ52_06880"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNG59533.1"
                     /translation="MKTLHTITRYAGVLFFASQFAGCGGDPSASSSSSSSSTSSSSSS
                     SSSSSSSSSSSSSSSSSSSTSSSSSSSSSGTAHPCASTDAAAFQAYARAIPGTIETED
                     FDPAGFLDSSAENQGNSYRTDTAVDIKAISGGNAIGWMTSGEWLEYTINVERAGTYDV
                     TIRAGAISLGRSLSISQCNSTLLANFEVPKINEWGQFKTWSAGKITLQPGRQKIRITV
                     TGGDDMDIDWIHIGPYTGTIDPPNPAILGATPPMGWNSWNTFGCSISETLIKQTADAM
                     VSNGMKDAGYQYVNMDDCWMNGRDANGKLQWDNTTFPSGIPALAQYVHGKGLKLGIYQ
                     SANQWTCVGVYGSAADKTKRVGSLGHEAQDATTFAEWGIDYLKYDLCAGQRASIIKMG
                     QAIRAQTRPILYSINPGNGQNDLDPPKQNWDMAGVANVWRIGFDINASWSSVMRLLDE
                     NSKLYSYAGPGGFNDPDMLEVGKLANINEDRSHFALWALMAAPLIAGNDIRNMSANTK
                     AILTNSEIIAVNQDVLGIQGRVVATPGQDLQVWSKTLSGNNKRAVVLFNRSNNTASIT
                     VKWSDLGLPAGAATVRDLWSHTDLGRFSDSYTATNIPAHGSGMVIISSAN"
     gene            complement(3070..>3285)
                     /locus_tag="PKZ52_06885"
     CDS             complement(3070..>3285)
                     /locus_tag="PKZ52_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59534.1"
                     /translation="LLPVACCLLPVACCLLPVGWILLSAISAVFKYRRKNIGSAFFTP
                     NQGKSPLNQHSNASLGPHQYSKRRKGR"
     assembly_gap    3286..3385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3837..5042
                     /locus_tag="PKZ52_06890"
     CDS             3837..5042
                     /locus_tag="PKZ52_06890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNG59535.1"
                     /translation="MALISCGGTTGTGSSSGSSSTSSNSSSSNSSSSSSSSTSSSSSS
                     SSSSSSSSSSSSSSSSSSNGGAPITIAPNNSLIRYNGRVALSAAAAEYDWANTQIEFN
                     INARQLELLINDGKNDYNLFIDGKLEKVISTTSSQSYPVTLAQGSHRVLLTKRTGPNF
                     GSGQFKGIRLPQGGELLALPATPNRRIEFIGDSHTVGYGNEGPALDCKGVYRPYENSY
                     LSFATIAARTLNADSRSIAISGFGAVRNYADTNTTSAMPMPYYYNRTLMNRADLTWNF
                     KNWVPEVVVIKLGTNDYSTQPYPAAQVFIQGIHDLINQVRTAYGTQTRIFLVADNSLA
                     QPVANMQTAVQQQRSMGFDRVHFVQLTFPPQNQLGCDWHPSVAGAEAMASQLVAAIKP
                     IMGWTDTAQ"
     gene            5429..6103
                     /locus_tag="PKZ52_06895"
     CDS             5429..6103
                     /locus_tag="PKZ52_06895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG59536.1"
                     /translation="MTQCPVGEPQCPVIDRLEALEAQIQELTAQTRTDNLTSLFNQRH
                     LMICLHQEIERTQRTQLPTSLILLDIDHFKKINDTYGHAVGDQALMHLANLLRNAVRK
                     LDIPCRQGGEEFAVILPSTPILVAKQIAERVRQALVENPLPLPNGALEVRASFGVSSF
                     AANSAKSLAQWLEETDQLLYEAKRAGRNCVKSAVHSMANPSVVSLEEKALLAQAGATP
                     SSNRVE"
     gene            6129..7244
                     /gene="mnmA"
                     /locus_tag="PKZ52_06900"
     CDS             6129..7244
                     /gene="mnmA"
                     /locus_tag="PKZ52_06900"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNG59537.1"
                     /translation="MSVKSCKVIVGMSGGVDSSVSAYVLKQQGYQVEGLFMKNWDEDD
                     GTEYCTAKADLADAQQVCNTLGIKLHTANFAADYWDNVFEHFLAEYRTGRTPNPDILC
                     NREIKFKVFLEYARILGADKIATGHYARTLTTASETYLLKGLDNNKDQSYFLHAVGET
                     EFSQTLFPIGDIAKPLVRKIAEEQNLITYNKKDSTGICFIGERRFKDFLQTYLPAQPG
                     LIQDDKGNVLGEHAGLMYHTIGQRQGLGIGGVAGAGEAPWYVAQKDLAHNILRVVQGS
                     DHPLLFAQGLNASQVHWINPEFAAAIQVDTPFECTAKTRYRQADQKCSVTVDSNNHLT
                     IMFKQPQRAITPGQSVVFYLGERCLGGAVIDHAFNSN"
     gene            7254..7901
                     /gene="hflD"
                     /locus_tag="PKZ52_06905"
     CDS             7254..7901
                     /gene="hflD"
                     /locus_tag="PKZ52_06905"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HNG59538.1"
                     /translation="MELNRWQQMALSLSGVIHAVILVDKIAQHGSIANADLEPCLRTL
                     FKRQPKDTLDVYGQLGFLLNSMEVLHSLLTQQCNPTHAHLLRYCMGVLHLQKKLSKNT
                     KMLAEIGAKLDKAATQVDMFGLNSDNLVANLAETYSTTISTFSFRIQVVGEANYLQQP
                     RLANLIRSLLLAAIRSAMLWHQVGGKRWHFIFYRKQLTQALEDLIKAAKSEIVNH"
BASE COUNT         2064 a         1989 c         1853 g         1987 t
ORIGIN      
        1 ctgcatggat gcaggaggta gagcaatgca ggagcaattg ccgagtttac acgcaacaaa
       61 cgaggatttt tgtcacaatt taacgccgcc aacgctcgca aggtaatatt tcaacaggct
      121 gctggacaga gtcagcagca attttgtgta gatgcacatc cttgtgcgct aatcaggctt
      181 taattagcac tgctgataat caccatcccg ctgccatggg cgggaatatt ggtagccgtg
      241 tagctatcgc tgaagcggcc taaatctgta tggctccaga ggtcgcgcac tgttgcggcg
      301 cccgctggca ggcctaaatc actccattta accgtgatgg aggctgtatt gttgctgcgg
      361 ttgaataaaa ctacggctct tttattattg ccggagaggg ttttcgacca cacctgtaaa
      421 tcctgccccg gcgttgccac cacacggcct tgaatcccca atacatcctg attcacggca
      481 ataatttcgc tgttggttaa tatggctttg gtattggcgc tcatattgcg aatatcgtta
      541 cccgcaatta gcggtgctgc cataagcgcc cagagggcaa agtgggaacg gtcttcattg
      601 atattggcaa gcttgcctac ttccaacata tccgggtcat taaaaccacc ggggcctgcg
      661 taggaataga gttttgaatt ttcatccagc aggcgcatta ccgagctcca gctggcatta
      721 atgtcaaaac ctatgcgcca aacattggca actcccgcca tatcccaatt ttgtttgggc
      781 gggtccaaat cattttggcc attacctggg ttgatgctgt acaggatggg ccgtgtttgg
      841 gcgcgtatgg cctggcccat tttaattatc gaggcgcgct ggcctgcaca taaatcgtat
      901 ttcaaataat cgatgcccca ctcggcgaag gtagtagcat cttgagcctc gtgacctaaa
      961 ctgcccaccc gttttgtttt atcggcggca ctgccgtata cacccacgca agtccattgg
     1021 ttggctgatt ggtaaatacc cagttttaaa cccttgccgt gaacatactg cgccaaggca
     1081 ggaatgcccg aaggaaaggt ggtgttatcc cattgcagtt tgccgttggc atcgcgcccg
     1141 ttcatccaac aatcgtccat attcacatat tggtaacctg cgtctttcat gccgttgctc
     1201 accatggcat cggcggtctg tttaatgagg gtttcgctga tgctgcagcc aaaggtattc
     1261 caggagttcc accccatagg tggggttgcg cctaaaattg ccgggttggg tggatcgatg
     1321 gtgccggtgt aggggcctat gtgaatccaa tctatatcca tatcgtcgcc accggtaaca
     1381 gtaatgcgaa ttttctggcg acccggttgc agcgtaattt ttcctgcaga ccaggtttta
     1441 aattgtcccc actcatttat ttttggcacc tcaaaattgg ctaataaggt gctgttgcac
     1501 tggctgatgg ataggctgcg gccaaggctt atagcgcctg cgcggatggt gacatcatag
     1561 gtgcctgcgc gctccacatt aatggtgtac tccagccatt ctccgctggt catccagccg
     1621 atggcattgc cgccactgat agctttgata tcgaccgcgg tatctgtgcg gtaactattg
     1681 ccttggtttt ccgcgctgga atccaaaaat cccgccgggt caaaatcttc agtctcaatg
     1741 gtgccgggta ttgcccgagc ataggcttga aatgcagcag cgtcggtgct ggcgcagggg
     1801 tgcgctgtgc cactggaaga ggatgagctg ctcgacgagg ttgagctgga tgaactactg
     1861 ctactggaac tactgctact ggaactactg ctactggagc ttgaagacga actggacgta
     1921 ctgctggatg aagaactcga tgaggcggaa gggtcaccac cgcagccggc aaattggctg
     1981 gcaaaaaaaa ggacacccgc atagcgggtt atggtgtgca gggttttcat ggtgtttccc
     2041 gatgtttatt gtcattttgg ggttgggttc ggcctagggg gctgccgctg aggaatgctt
     2101 aaatccatgt ggactttatc ttagggggtt atggttgaaa tgatattaat tggtgcacat
     2161 ttttgtaaga tggtaagaca ataaaggagg accaatcgca gcggggggat gcaacacagg
     2221 cggggatgca gaatacaaaa ccatcccccg taaagttcgg gctgcgccgc taaataaagc
     2281 caaagcatct attaagtaaa attttgacca cagccctgtg tttaagctgg ccgctcacac
     2341 gggtgtaaaa caacaaatat aaaaggcgtg tgtaaacgat ttttggggtt tttggggttt
     2401 ttagggtttc gggcagatta actgtgcaaa gttgaatttg cgctgatcat agtattcagc
     2461 cctagaattg gccgctgatg tgataagtag tcgaatactt cgtgtacgct tgcaatttta
     2521 cctgcaggta aattaaatcc aactcgtcta aacacacagt tatgcaactt catttaaaag
     2581 tgcgcggcga acttctcaca caaatatcgg cactatggtt gatatggtgc cgtcagtttg
     2641 aaacgtagct aaaggttgct ttttgcccag tgcaaattaa atatctagaa taatctggcc
     2701 acttacatac accaatattt tcataactat tcagtacccc gtaatttata tttacctgtc
     2761 tgttcgttgc ttgcggtgtg ttgcccgggt gcaatctgcg gcaaggggcg gaagtgcatt
     2821 tggatcctgc gactacgcca ggatgacgcg ttcaaaacgc ccagataact cgtttaaaaa
     2881 tgcggaagat ggcaggctgc taaatttcac ccaactgtta atctgcataa gccaaccgtc
     2941 attctgcaca acccaatcgc cattctgcag aacctaactg ttattctgta taaccgaact
     3001 gtcatcctgc gcgcagtcgc aggatccatt tatgtttagg ggtaagtgtt tgataccctc
     3061 tgattaattt tatctgcctt ttcgtcgctt gctgtattga tgcggcccta gcgaggcatt
     3121 ggaatgctga tttaaaggac ttttaccttg gttaggagta aaaaatgctg aaccaatatt
     3181 ttttcgtcga tatttaaata cagcactaat agcagacagc aaaatccagc caacaggcaa
     3241 caggcaacag gcaacaggca acaggcaaca ggcaacaggc aacagnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnncaaca ggcaacaggc aacaggcaac aggcaacagg
     3421 caacaggcaa caggcaacag attgcgacag caatacaatc gcaccagcat ttgtatttct
     3481 tattaaccca gcggcaaaac agagagtacc gatccattaa ttggcgattg cgaattaacg
     3541 cgcaatcaat aggctaaggt tttaataatt ttttagcgtc tacactgaag cctggcagcc
     3601 aaagaaatac cgggtgaagc cgaccaaact gctaacagtg tgtcgcagat aactattttt
     3661 gcctgtatcc aatgataata tccacttatt cagctgaatc tcagccgcca cttgaatacg
     3721 cgtttctcag gcgtttaaaa attgtgataa taataaaaga gcacccattt atgttgcata
     3781 gactgaaaac tgccttacac ccaaccctta atatgagtgc tgcatcaata ctatgcatgg
     3841 cgttaataag ttgtggcggt acaacgggca ctggctcatc cagcggcagc tccagtactt
     3901 cctccaattc aagttcgtca aactcttcta gctcgtccag ctcaagcacc tctagttcca
     3961 gttcctcaag ctccagctct tcgagcagtt caagttcatc gagcagttcc agttctagct
     4021 ctaatggcgg ggcgcccata accattgcgc cgaataattc gttgattcgt tataacggcc
     4081 gagtagcatt aagtgcagcg gcggcggaat atgattgggc caatacacag attgaattta
     4141 atattaatgc acggcagctg gaattattaa tcaatgatgg caaaaacgat tacaacttat
     4201 ttatcgatgg caaattggaa aaggttattt caactaccag cagccaatcc tacccggtaa
     4261 ccctagcgca aggctctcac cgagttttat taactaagcg caccggcccc aatttcggct
     4321 cggggcaatt taaaggtatt cgtttacccc aagggggcga gcttctcgcc ctgccggcca
     4381 cgcccaatcg aagaatcgaa tttattggcg attcccatac agtgggttat ggcaacgaag
     4441 gcccggcttt ggattgcaag ggtgtgtata ggccctatga aaacagttat ctgtcttttg
     4501 caaccattgc ggcccgcaca ctaaatgctg atagccgctc gattgctatt tctggttttg
     4561 gcgccgtgcg taattacgct gacaccaata ccaccagcgc catgccaatg ccctattact
     4621 acaatagaac actcatgaac cgcgccgacc tcacctggaa ttttaaaaac tgggttcctg
     4681 aagtggtggt gataaaatta ggcaccaatg actactccac ccaaccctac ccagctgcgc
     4741 aggtatttat acagggcatt catgatttaa ttaatcaagt gcgcaccgcc tacggcacac
     4801 agacgcgaat tttcctggta gccgataata gcttggcgca acccgtcgct aatatgcaaa
     4861 cggccgttca gcaacagcgt tcaatgggtt ttgatcgagt tcattttgtc caattaacct
     4921 tcccgccgca aaatcaatta ggttgtgatt ggcatccgtc tgttgcaggc gctgaggcaa
     4981 tggccagcca attagtcgca gctattaaac ccattatggg ctggacagac acagctcaat
     5041 aagttgccag ttaggtggta ataattatca acagccgccg aaatgcccag tacgtaggct
     5101 aagaactgga gaatttagca attagtattt cctataaaag cgcgccgata ataattaccg
     5161 gcgcgttttt ttatttgcaa gctctacaac gcgagccgct ggtgcactag agttttttta
     5221 caatcagccg cacttaacaa ttaccacctg tttagccaga tcaggatccc ccaaaggcgg
     5281 ctagtccaaa ctttacactt ggagcctaaa tcccgacctc agggtctagc aactttgatt
     5341 ttttgtgcct tcaaaatgcc ctcagcaatg cccctgctgt taagatacgc acacatttta
     5401 ttgttcatag atatcgagga cctgcgccat gacccagtgc cctgttggag aaccgcaatg
     5461 cccggtcatc gaccgcttgg aggcattgga ggcgcagatc caagagctta ccgcccaaac
     5521 ccgcacggac aacctaacct cactctttaa tcagcgccac ctaatgatct gcctgcacca
     5581 ggagattgaa cgcacccagc gcacccaact gcccaccagc ctgatcctcc tcgatatcga
     5641 tcacttcaaa aagataaacg atacctacgg ccatgcggtg ggggatcaag cgcttatgca
     5701 tttggccaat ctattgcgca atgcggtgcg caaattggat atcccctgcc gccagggcgg
     5761 tgaggaattc gccgtaattc tgccctcaac ccctattctg gtggccaaac aaatcgccga
     5821 acgggtgcgg caagcactgg ttgaaaatcc cctacccttg ccaaacggcg ccctagaggt
     5881 gcgggccagt tttggtgtct cttcatttgc ggccaacagc gccaaatccc tagcccaatg
     5941 gctggaggag accgaccaat tgctctatga agccaaacgg gccgggcgca actgcgttaa
     6001 atccgccgta cacagcatgg ccaaccccag cgtggtgagt ttggaagaga aggccctgct
     6061 ggcacaagcg ggcgccacgc ccagttcaaa tcgggtagaa tagcgccccc tttcttccgt
     6121 gcgtttgtat gtctgtcaaa tcctgcaaag taatcgttgg catgtccggc ggcgtagatt
     6181 cttctgtctc agcttatgtg ctcaaacagc agggctacca ggtggaaggc ctatttatga
     6241 aaaactggga tgaagacgac ggcaccgaat actgcaccgc caaggccgat ttagccgacg
     6301 cccaacaggt atgcaacaca ctgggcatta aattacacac tgctaacttt gccgccgatt
     6361 attgggataa tgtttttgaa cattttttag cggaatatcg cacaggccgc acacccaacc
     6421 cggatatttt gtgcaaccgg gaaattaaat tcaaagtatt tttagagtac gcccgcatat
     6481 taggcgccga caaaattgcc accggccatt acgcccgcac cctcactacc gctagcgaaa
     6541 cctatttact taagggtttg gataacaaca aagaccaaag ttattttttg cacgcggtgg
     6601 gcgagacaga atttagccaa accctgtttc ccatcggcga catagctaaa cccctagtgc
     6661 gcaaaattgc tgaagaacaa aacctaatta cctacaataa aaaagacagc accggcattt
     6721 gttttattgg tgaacggcgt tttaaagatt ttttacaaac ctatttaccg gcccagcctg
     6781 ggctgatcca agatgacaaa ggcaatgtcc tcggcgaaca cgcaggcttg atgtaccaca
     6841 ccatcggcca acgccaaggc ctgggcattg gcggtgttgc cggtgcgggg gaagcgccct
     6901 ggtacgtggc tcagaaagat ctcgcccata atattttgcg cgtagtgcaa ggcagcgacc
     6961 atccattatt attcgcccag ggtttaaatg cctcgcaagt ccattggatc aacccagaat
     7021 tcgcggcggc aattcaagtt gatacgcctt ttgagtgcac ggcaaaaacc cgttaccgcc
     7081 aggctgatca aaagtgtagc gtcacggtag atagtaacaa tcacctcacc attatgttta
     7141 aacaacccca acgggccatt acaccgggcc aatcggtggt attttattta ggtgagcgct
     7201 gcttgggcgg cgccgtcata gaccatgcgt ttaattctaa ttagaggtgt actgtggagt
     7261 taaatcgttg gcaacaaatg gcgttaagcc tatccggcgt cattcacgcg gtaattttgg
     7321 ttgataaaat cgcccagcac ggctccatcg ccaatgcaga tttagagccc tgtttgcgca
     7381 cactgttcaa acgccaaccc aaagatactc tggatgtata cggccaattg ggctttttac
     7441 tcaacagcat ggaagtgtta cacagcctac tgacccagca gtgcaaccct acccatgccc
     7501 atttattgcg ctattgcatg ggtgttttac acctgcaaaa aaaactcagc aaaaatacta
     7561 aaatgctcgc cgaaattggt gcaaaattgg ataaggccgc cacccaggtg gatatgtttg
     7621 gcctcaattc cgacaaccta gtggcaaacc tggcagaaac ctacagcacc actattagca
     7681 cctttagttt taggatacag gtagtaggcg aagctaatta tctacaacag ccgcgtttag
     7741 ccaatttaat ccgcagccta ttattagccg ccattcgttc ggccatgtta tggcatcaag
     7801 tcggcggcaa gcgctggcat tttatttttt atcgcaagca acttactcag gcgttagaag
     7861 atttaatcaa agccgccaag agtgaaatag ttaaccacta aatacagtga attttatgtc
     7921 taccaactcc ctccccctat cgtcgttaac cgcagtatcc cctttagatg gccgctacag
     7981 cagtcgcacc gcc
//
LOCUS       DAOQGS010000137         7974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4316_length_7974_cov_4.907312, whole genome shotgun sequence.
ACCESSION   DAOQGS010000137 DAOQGS010000000
VERSION     DAOQGS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_7974_cov_4.907312"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2626
                     /locus_tag="PKZ52_06910"
     CDS             <1..2626
                     /locus_tag="PKZ52_06910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNG59539.1"
                     /translation="SMSDDDRTQFMPPRREAPQSPPPTKPAPPGPPTPAPPAADLTKV
                     QTPRPLESTPEGENLVAEFITLLQPVGSNRQKRPISEVAKPAAATEMVTQIAGTKPRE
                     QAQSRILKGRFVIEEILGAGGMGVVYKAKDLLKVEAQDREPYVAIKVLGEAFKEHPEA
                     FISLQRESRKTQKIAHPNIVNVHDFDRDGETVFMTMEFLDGKPLDKLITQYKATGLLP
                     EDAHKILRDITAALIHAHRHKIIHSDFKPGNIFVLKNSTAKVLDFGIARAAAIADMSA
                     SANEEDHTLFDAGSLGALTPAYASLEMLKGETPDARDDIYALGCIAYEMFTGQHPFNR
                     KPATEALSLQLKPKKIPFLNKRQWRAIELALAFHRSDRVPTVEEFWQLFSYKNTQKNA
                     VAWVLFSALIGAGGAAYYFWPEPQQGPDVLSIEKRLRQELSEKAIKKLLAEPSFTPGW
                     QEQLWSEMAEAKQLVGEQNTWWQGLRQKIIDLYVPKIDEAIKKDALSDAQALFKSYER
                     YGPDQQQALDIQSKIAALAAKIQEAEQKRLAEAQQRAQQLAQQQAQNAAATPLKTPDT
                     APAKANDAGDHYKVALDTVTNQLSCATTPDMRDLEISVGKLKSINAQTYAKDEAKIIA
                     ALAGCVKKISEASVPRAEELKKMALRLFPGNALLSGLQFAARDNCPLSLAGLGGTGAR
                     GSCKDTLSDGGRTPNLVVVPANGELKAFAIGKYETTVDEFNGYCKSSGRCSPIAGDGN
                     LPATQISLSQAQGYAEWLSKKTGKTYRLPTRAEWVYAARAGSEQTDPNRNCSLNSRGI
                     QKGDALVGATVGGANRWGLVNHIGNAREWATSGGSVAALGGSRETAMDQCTASSATPH
                     DGKADNLTGFRLVREISQ"
     gene            complement(2616..3392)
                     /locus_tag="PKZ52_06915"
     CDS             complement(2616..3392)
                     /locus_tag="PKZ52_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59540.1"
                     /translation="MNHLNQCPEQIAIDELTQRRLFNQVGKPLEQTPQSLEQAFSWQQ
                     AVAAQMAVQGRPVVGWKCALPSAERWGLAPIFQLHRGASVALPAHSPRALVEPEFAWR
                     VRQDLLPGQTYSPEALRQALQCHLALEIIFDRFEAGARVDYYSRLADGLLNHSLWLGP
                     PLPEAPAQLPLRWQVGAERAAVEGVHPSGDAFAPAEWLVAFLHQRGMGLYAGQVIITG
                     SLNGLWRLPVNQPVVIEYADLARIEVEFTSEVCALDGATG"
     gene            complement(3674..5968)
                     /locus_tag="PKZ52_06920"
     CDS             complement(3674..5968)
                     /locus_tag="PKZ52_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNG59541.1"
                     /translation="MTANTIYAQIAHELNVATAQVSAACQLLDEGATVPFVARYRKEV
                     TGGLDDNQLRLLAERLDYLRELSARKTSILKSIAEQGKLTPELERAIGQAHTKTALED
                     LYLPYKPKRRSKAQTAKEAGLEPLALGLLHNPSLEPLAAAKAYINPDFSINTEADALE
                     GAKHILIELFSETAPLLEQLRQHQKEYAWVEVRVAKGKESAAAKFQDYFDYREPWKKI
                     PSHRALAIFRGEKEDLLVVSLKHPEEAIAHPCIGIIAAQFSLADRKRAADSWLMAVVV
                     AAWQQKLAKNIESDLMGELREKAEHEAIAVFARNLKDLLLAAPAGQKAVLGLDPGLRT
                     GVKLVVLSATGQVLEHGAIFPHPPHHKIAEAAAVVVKLIKKHQVQLIAIGNGTASRET
                     EKFIADILKAQPDLTAQKIVVSEAGASVYSASELAAKEFPELDVTIRGAISIGRRLQD
                     PLAELVKIDPKAIGVGQYQHDVSQTHLARALDAVVEDCVNAVGVEVNTASAPLLARVA
                     GLNATIANNIVAFRNEKGPFKNRQQLLKVPRLGAKAFEQAAGFLRIHQAPNPLDASGV
                     HPESYPVVEAIAAKHQRSIAALIGDSSFLRSLKPSDYANAQFGVPTVTDILKELDKPG
                     RDPRPEFKTAQFKDGIEKITDLQPGMILQGVITNVTNFGAFVDVGVHQDGLVHISMLA
                     HQFVKDPHAVVKAGEIVAVKVMEVDAARKRIALSMRLDDVPGQEAKPVATKHQAAGPK
                     QNNPAPATGTMAALFSQALQKKKP"
     gene            complement(6087..>7974)
                     /locus_tag="PKZ52_06925"
     CDS             complement(6087..>7974)
                     /locus_tag="PKZ52_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG59542.1"
                     /translation="PIYQKVVQGKDLVADILPPPAYIIEANLLAHQLAFVTSSEQKNL
                     IAQVEQLQRDFIARNQFWQQQDIPPAIKALLTSAAAPAERFFTAFSGRLAPAIKSGET
                     ARAADILKDMQADYSAHRQAIDKLVAELAVVNKQVEEAAVQSVSDTRWELGGLFLLIS
                     VFCLAAAQWSRIKAIALIGGELSTALYWVGRLGDGDLRQQPRNPPAGSILAAFGSLTD
                     KINRVLQGIDSTNREVSQSIYQVMSVSKQISQVSTDRQRGADLVNQTTQGLLTGLNRV
                     KHLVEDTQSRTQNAANKAQQGLNAVSQIRTHVAQTVDKVLHSESCMRELAAATGEIHS
                     IVSSIKAIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLATRTGEATAQITQLV
                     SGLNSKVDTTLLTMCQVAEVVTQVQSYTDENDESISQIVHTAQHNHAASSEILQATQE
                     QLNHLERLSHQVEDLFSALKFSDTTLKVTATISNALEKTVADLQAQIGFFKFIPNTLE
                     DPHPNTKREHPRLRNSLLVMAGRPGGQKVQGVTVDFSLGGMRLATPTYINADKQDLIE
                     LQIKPPVDQLNAYLNRPPIILQGRIVRVHQEGDEIHYGISFEDLESDQQRELELVQSF
                     YLAA"
BASE COUNT         1896 a         2275 c         2022 g         1781 t
ORIGIN      
        1 ctccatgagt gacgacgacc gcacccagtt tatgccgccc cgccgggagg ccccgcaatc
       61 cccaccccct accaaacctg ccccgcccgg cccgccaaca cccgccccgc cagcggccga
      121 tctcaccaaa gtgcaaacgc cgcgccccct ggagtccacc cccgaggggg aaaacctagt
      181 ggcggaattt ataacgctgt tgcaaccggt gggcagcaac cggcaaaaac ggcctatttc
      241 cgaggtggcc aaacctgccg ccgccaccga aatggtgacg caaattgccg gcactaaacc
      301 ccgcgaacag gcccagtcgc gcattttaaa aggccgcttt gtgatcgaag aaattctcgg
      361 cgcaggcggt atgggtgtgg tgtacaaagc gaaggattta ttaaaagtag aggcccagga
      421 tcgcgagccc tatgtggcga ttaaggtatt gggcgaagcc tttaaagaac accccgaagc
      481 gtttatttcc ctgcagcggg aatcgcgcaa aacccaaaaa attgcccacc ccaatattgt
      541 caacgtacac gacttcgacc gcgacggcga aaccgtgttt atgaccatgg agtttttaga
      601 cggcaagccg ctggataaat taattaccca atacaaagcc accggtttat tgccggagga
      661 cgcccataaa attctgcgcg atattaccgc cgccctcatt cacgcccatc gccataaaat
      721 cattcactcg gattttaagc ccggcaatat ttttgtactg aaaaattcca ccgccaaagt
      781 actggatttt ggtatcgccc gagcggcggc cattgccgat atgtccgcct ccgccaatga
      841 agaggaccac acattattcg acgcgggaag cttaggtgct ttaaccccgg cctacgccag
      901 cttagaaatg ctcaaaggtg agacccccga tgctcgcgac gatatttatg ccctcggctg
      961 tatcgcctat gaaatgttta ccggccaaca cccatttaat cgcaagcccg ccaccgaagc
     1021 cttaagcctg caattaaagc cgaaaaaaat ccctttttta aataaacgcc agtggcgcgc
     1081 cattgaactt gccctggcct ttcaccgcag cgaccgcgtg ccgacggtgg aggaattttg
     1141 gcaattattc agctacaaaa atacccaaaa aaatgccgtc gcctgggtgt tatttagcgc
     1201 cttaattggc gcgggcggcg ccgcctatta tttttggccc gagccccaac aggggccgga
     1261 tgtgttatcc attgaaaaac gcctgcgcca agaattaagc gaaaaagcca ttaaaaaatt
     1321 attagccgaa cccagcttta cccccggctg gcaggagcag ttgtggtcgg aaatggcgga
     1381 agccaaacaa ttagtgggcg agcaaaatac ctggtggcaa ggtttgcgac aaaaaattat
     1441 cgatttatat gtacccaaaa tagacgaagc gattaaaaaa gacgccctaa gcgatgccca
     1501 ggcattattt aaaagttacg agcgctatgg gccggatcaa caacaggcgc tggatattca
     1561 aagcaaaatc gccgcgcttg ccgccaaaat tcaagaagcc gaacaaaaac gcctcgccga
     1621 agcccagcag cgcgcccaac aattagccca gcaacaggcg caaaatgcgg cggcaacccc
     1681 gctgaaaacg cctgatacag cgcccgccaa agccaatgat gcgggggatc actacaaggt
     1741 ggccttggat accgtcacca atcaattaag ctgtgctacc acaccggata tgcgcgactt
     1801 ggaaatatcc gtcggcaaat taaaatccat caatgcacaa acctacgcca aagacgaagc
     1861 caaaattatt gccgccctgg ctggctgtgt gaaaaaaatt agtgaagcgt cggtgccccg
     1921 cgcggaagaa ttgaaaaaaa tggccctgcg cctattcccc ggcaacgcac ttttgagcgg
     1981 attacaattt gccgcccggg ataattgccc gctgagttta gcgggccttg gcggcacggg
     2041 tgcacggggc agctgcaaag ataccctgag cgatggcggc cgcacgccaa acctggtggt
     2101 ggtgcccgcc aacggcgagt taaaagcctt cgccatcggc aaatacgaaa ccaccgtgga
     2161 tgaatttaac ggctactgca aaagctccgg tcgctgcagc cccatcgccg gggacggcaa
     2221 cctgcccgct acgcaaatta gcctcagcca agcccagggt tatgccgaat ggctgagtaa
     2281 aaaaaccggc aaaacctacc gcctgcccac ccgcgccgaa tgggtgtacg ccgcccgcgc
     2341 cggcagtgaa caaaccgacc ccaaccgcaa ctgcagcctc aactcccgcg gcattcaaaa
     2401 aggcgacgcc ctggtgggtg ccacggtggg cggcgccaac cgctgggggc tggtgaatca
     2461 catcggcaat gcccgcgagt gggccaccag tggcggcagt gtggcggctc tgggcggttc
     2521 ccgggaaacc gccatggatc aatgcaccgc cagcagtgcc accccccacg acggcaaggc
     2581 ggataacctc accggatttc gcctggtgcg ggaaatcagc cagtagcgcc gtctaaagca
     2641 cacacctcgg aggtgaattc cacctcgatc cgggccagat cggcgtattc gatgaccacc
     2701 ggctggttca ccggcaagcg ccacaggccg tttaaggagc cggtgatgat cacctgcccg
     2761 gcgtacagac ccatgccgcg ctggtgtaaa aacgccacca accattcggc cggagcaaag
     2821 gcatcgccgc tggggtgcac cccttccacc gccgcccgct cagcgcccac ctgccagcgc
     2881 aggggcaact gcgcgggcgc ctcaggcagg ggcggcccca accacaggct gtgattgagc
     2941 agaccatcgg ccaaacggct gtaatagtcc accctggcac cggcttcaaa acgatcaaaa
     3001 ataatctcta aggccaaatg gcactgcaag gcttggcgca gcgcctccgg gctataggtt
     3061 tgccccggca gcagatcctg ccgcacccgc caggcaaatt caggctccac taaagcgcgc
     3121 gggctatggg caggcagcgc cacacttgcg ccccgatgca actggaaaat aggcgccaaa
     3181 ccccagcgct cggcgctcgg cagggcacat ttccacccca ccacaggccg cccctgcacc
     3241 gccatttgcg ccgccaccgc ctgctgccag gagaaggcct gctctaagga ttgcggggtt
     3301 tgctctaggg gcttacccac ttggttaaac agccgccgct gggttaattc atcaatagca
     3361 atctgttcgg ggcattggtt taaatggttc acggggcgat ctcacggctg accataacca
     3421 agagcgcaac ctgccaacac caaggcaggc agggtctctc gatttgtata atggaattta
     3481 taaatggcta ggcacatatt aacccggcgg caaaataaat cgtcctgatt tattttgcct
     3541 tcgccgcact aaacctacga aaatgcgcgg attttcttgt tttagtgcgg ctcgcggcgg
     3601 tttatacagc cgaggcggca tccctgccgc ctttattaac ccggcactca ttgccgccgg
     3661 gttaataaca ggcctagggt ttcttttttt gcagggcttg gctgaataat gccgccatag
     3721 tgcccgtggc aggcgcagga ttattctgct tggggcctgc cgcctggtgt ttggtcgcca
     3781 caggtttggc ctcctggccg ggcacatcat caagccgcat ggagagcgca atccgcttgc
     3841 gggcggcatc cacttccatc actttcaccg caacgatttc gcccgcctta accactgcgt
     3901 gggggtcttt aacaaattgg tgggcaagca tggaaatatg cactaaacca tcttgatgga
     3961 cgcccacatc cacaaaggcg ccaaaattcg tgacgttggt aattacgccc tgcaaaatca
     4021 tgcccggttg caaatcggta attttttcta tgccgtcttt aaattgcgcg gttttaaatt
     4081 ctgggcgcgg gtcccggccg ggtttatcca attcttttaa aatatccgta accgtgggca
     4141 caccaaattg tgcattggca taatcgctgg gttttaagct gcgcaaaaaa ctgctgtcgc
     4201 caattaatgc ggcaatgctg cgctgatgtt ttgccgcgat ggcctccacc accggatagg
     4261 attccgggtg tacccctgaa gcatccaggg gatttggcgc ttgatgaata cgcaaaaatc
     4321 ccgccgcctg ctcgaaagct ttggcgccca agcgcggcac ttttaataat tgttggcgat
     4381 ttttaaacgg gcctttttca ttgcgaaacg ccacaatatt attggcaata gtggcattta
     4441 aacccgccac ccgcgcaagc agcggcgccg acgcggtatt gacttccacc cccaccgcat
     4501 tcacacaatc ctccaccacc gcatccaatg cacgcgctaa atgggtttgc gatacatcgt
     4561 gttgatactg gcccacacct atggccttgg ggtcaatttt caccagctcc gccaaaggat
     4621 cctgcaggcg gcggccgatg gaaatagcac cgcgaatggt aacgtccaac tccgggaatt
     4681 cttttgcggc cagttcagag gctgaataca ccgacgcgcc cgcttcgctc acgacaattt
     4741 tttgcgccgt taaatcaggc tgcgctttta aaatatcggc gataaatttt tccgtctcgc
     4801 ggctggccgt gccattgcct atggcaatta attgcacctg gtgtttttta atgagtttta
     4861 ccacaaccgc tgcggcttca gcgattttat ggtggggcgg atgagggaaa atagcgccgt
     4921 gttcgagcac ttgcccggtg gcgctcagca ctaccagttt tacgcctgtg cgcaagcctg
     4981 gatctaaccc caataccgct ttttgcccgg cgggagcggc taataataag tcttttaaat
     5041 tgcgggcaaa tacggcgatg gcctcgtgct cggctttttc ccgcagctca cccattaaat
     5101 cgctttcaat atttttggct aatttttgct gccaggccgc aaccaccacc gccattaacc
     5161 agctatcagc tgcgcgttta cgatcggcca gggaaaattg cgcggcaata ataccgatgc
     5221 acggatgggc aatagcttct tctggatgct taagcgacac caccaataaa tcttcttttt
     5281 caccgcgaaa aatagccagc gcgcggtggc tgggaatttt tttccagggt tcgcggtaat
     5341 caaaataatc ttgaaattta gccgccgccg attctttgcc tttagccacg cgcacctcaa
     5401 cccaggcgta ctccttttga tgctggcgca attgttccaa cagcggcgcc gtctcgctaa
     5461 ataactctat taaaatatgt ttagctccct ccagcgcgtc tgcttcagta ttaatactga
     5521 agtccgggtt tatataggct ttagccgctg cgagaggctc taagctgggg ttgtgcaaaa
     5581 gccctagcgc caatggctca aggcccgctt ctttagcggt ctgcgctttg ctgcggcgtt
     5641 taggtttata gggtaaatag aggtcttcta aggcagtttt agtgtgcgcc tgtccaatag
     5701 cgcgctcaag ttcgggggtt aatttcccct gctcggcgat ggattttaaa atgctagttt
     5761 tgcgcgcgga taattcccgt aaataatcca agcgctcggc cagtaggcgc aattggttgt
     5821 cgtctaggcc gccggtaact tctttgcggt agcgggccac aaacggcacc gtggcgcctt
     5881 catccaataa ttgacaagcg gcgcttacct gtgcggtagc cacatttaat tcatgggcta
     5941 tttgggcata aattgtatta gcggtcataa tcacctcgtg ttgctggcgg caattatgct
     6001 tggcttaggg ggactttaat agccccaagc cccggttgag tcagggcttg ggggcacata
     6061 atcgggcggc attgcctcaa gccattttat gcggcgagat aaaaagactg caccaattca
     6121 agctcacgtt gttggtcgga ctctaggtcc tcgaaggaaa tgccgtaatg aatttcatcg
     6181 ccctcttgat gaacccgcac aatacgcccc tgcaatataa tcggcgggcg atttaaatag
     6241 gcattgagct gatccacggg cggcttaatc tgtaattcaa tcaggtcttg tttatcggca
     6301 tttatatagg taggtgtggc aaggcgcata ccgcccaaac taaaatccac cgtcacgccc
     6361 tgcacttttt gcccgccggg cctgcctgcc atcaccaata aactattgcg caagcgcgga
     6421 tgttcgcgct tggtattcgg gtgagggtct tctaaggtgt tagggataaa tttaaaaaag
     6481 ccaatttgcg cctgcaaatc cgccacggtt ttttccagcg cattggaaat tgttgcggtg
     6541 actttcaacg tggtatcgct gaatttaagc gccgaaaata aatcttcaac ttgatgactt
     6601 aagcgttcaa gatgatttaa ttgctcttgg gtggcctgca aaatttcact gctggcggcg
     6661 tgattatgtt gggcggtgtg gacaatttgc gaaatcgact cgtcattttc atccgtgtaa
     6721 ctttgcacct gggttaccac ctcggccacc tggcacatgg ttaataaggt ggtatccact
     6781 ttggaattta aaccgctcac caactgggta atttgcgccg tggcttcacc ggtgcgggtg
     6841 gctaaggtgc gcacctcgtc ggccaccacc gcaaacccgc gcccctgctc ccctgcgcgg
     6901 gccgcttcaa tggccgcatt taacgccaac aaattggtct gatcggcgat ggctttaata
     6961 ctgctaacta tggaatgaat ttcaccggtt gcggcggcta attcgcgcat acaggattcg
     7021 ctgtgtaata ccttatcaac cgtctgcgcc acatgggtgc gaatttgtga aaccgcattt
     7081 aaaccctgct gagctttatt agctgcattt tgtgtgcggc tttgtgtatc ttccaccagg
     7141 tgtttgacgc gatttaaacc ggttaataat ccctgcgtgg tttgattgac taaatcggca
     7201 ccgcgctgac gatctgtgct cacctgggaa atttgtttgg agaccgacat cacctgatag
     7261 atggattggc tcacttcgcg attggtgctg tctatgccct gcaaaacccg attaatttta
     7321 tcggttaaac tgccaaaagc cgccaaaata ctgcccgcag gcggattgcg gggctgctgg
     7381 cgcagatctc catcccccaa acgccccacc caatataacg ccgtggataa ctcaccgccg
     7441 atcagcgcaa tggctttaat gcgcgaccac tgggcggcgg ctaagcaaaa cacgctaatc
     7501 aataaaaata aaccgcccaa ttcccagcgg gtgtcgctga cactttgtac cgccgcctct
     7561 tcaacctgtt tattaactac tgccaattcg gcaaccaatt tatcaatcgc ctgccgatgg
     7621 gcactgtaat ccgcctgcat atccttaaga atatccgccg cccgcgccgt ctcaccggat
     7681 ttgatggccg gtgctaagcg cccgctaaat gcagtaaaaa aacgctcggc gggggctgca
     7741 gcagatgtca aaagagcttt tattgcagga ggaatatcct gctgctgcca gaattgatta
     7801 cgggcgataa aatcccgctg caattgctcc acctgggcga taaggttttt ttgctcgctg
     7861 ctggtgacaa acgccaattg gtgggcgagt aaattcgcct cgatgatata ggccggcggc
     7921 ggcagaatat ccgccaccaa atccttaccc tgtaccactt tttgatagat gggc
//
LOCUS       DAOQGS010000138         7959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4328_length_7959_cov_4.436108, whole genome shotgun sequence.
ACCESSION   DAOQGS010000138 DAOQGS010000000
VERSION     DAOQGS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4328_length_7959_cov_4.436108"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PKZ52_06930"
     CDS             complement(<1..1619)
                     /locus_tag="PKZ52_06930"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HNG59543.1"
                     /translation="MEHLKSTLSVRLAQYSAAGVKQENQDTLGARIPEGTALATKGIA
                     LAIADGVSSSVAGKEASQTAVAGFLSDYYATPDTWRTQQSAVQVIQSLNRYLFGKSQN
                     SLKQEGHLTTFSCVILKGDKAFCFHVGDSRIYRLRDNSFEQLTRDHAQKVDVKTTYLT
                     RALGADPVLEVDMSSYELEIGDIFVLTTDGVHDFVNAKLIAQCIKDAYDDLDSAAEGL
                     CQLALANKSQDNLSIQICRVEARGTASQADAVTVLSRLPFPPFLQVGHILDGLTIKKI
                     LHESERSQVYLVHTQDGTPLVMKTPSVNYEDDPVYIERFVLESWIGARIHNAHVVRVV
                     EPPNPRSCLYYLTEYIQGPSLTQLIKERAPFAIPDAIELVDQLIKGLRAFHRKDTLHQ
                     DLKPDNVIIGKRGAVIVDFGSCWVAGIQEAGATFERDTILGTLDYSAPEYRLGSHAGE
                     RSDLFSLGVLLYEMLTQKLPYGSAYSSAMDLKSLQKLNYISACAINPLVPFWLDKTLE
                     KALHINPERRYESLSEWLQDLKRPNPHLLTQR"
     gene            complement(1652..2797)
                     /locus_tag="PKZ52_06935"
     CDS             complement(1652..2797)
                     /locus_tag="PKZ52_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNG59544.1"
                     /translation="MIKILIVGAGIAGLALARRLTNTNVKVTIIERDSHFPVGGAGIC
                     LPANAVIQMRRLGLYDRLMNASHTVNLIEFAKSNGATITTASLGEPPLNIAEFVALPR
                     QRLLELLQYGMSNAVCFSSQVTKLSETEGGLWVHINHSSRHEFFDLVVAADGLHSTTR
                     QLSLKNSKPKSLGVTNWRFIAEHPERDAHPIYYLGNDDAFMVYPMGSTQSYCYAQIAD
                     PLNQWRQLPAIPTLQTLFGHYCAPVKSLLDSLKPDTKVMAGTLETVNVTRCFEGRTVF
                     IGDALHGCPPTLQQGVGMGLEDANLLADLITRFPVDDALQMYHEQRYPRVHWIVEESN
                     RLIELANKGKSWPGRLIRNTLLRQHGPANVAAWRRILSDSVHPELDF"
     gene            complement(3571..4674)
                     /locus_tag="PKZ52_06940"
     CDS             complement(3571..4674)
                     /locus_tag="PKZ52_06940"
                     /inference="COORDINATES: protein motif:HMM:NF037701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNG59545.1"
                     /translation="MTSFFFTFFILVVLLLNLSGCKNHSTNSQNSPAPVPPKPDQSTT
                     YSFDAQSLSIEGRYLTNTDHSITLASAASNVSFMAPGSKLEFLASAEFERAFIDILVD
                     GQLIQKAYVLDHKTNTVSINLGESAKNTHLVEIYNRGEGDSFATRLISLETEQTLIPI
                     TKPNRKLMFIGASVTCGTLSEPADNCALGPASHNGRVAHGTLIAHALNAESQLVCRSG
                     RGILNGSVASIPQDAVHFLDYILTTANGPVYWDHRLFTPDGILIALGNNDDLSNGERF
                     ISAYNTLLYKLRSLYPEALIILTEGPLISGERKNQLTAYLTSIVSASNDAKLSYIPSK
                     KYPNSPCNDHPDAKTHQAIAEELLPQIKEKLKW"
     gene            complement(4671..5552)
                     /locus_tag="PKZ52_06945"
     CDS             complement(4671..5552)
                     /locus_tag="PKZ52_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59546.1"
                     /translation="MLIIEYLLKTVLCLLLLAYSYMQQSIQFLKMTDEVFSASMEKKN
                     ATSGNKYDYIVVGGSNALTGVSARTISMKTNSNAYNLAISACEGAGLLDYPDWLSQSN
                     STTKSVIYSSMNIWHLARRSPCELYSDSLSNQPYHRPFNVIPLARTISNALQKMSFSI
                     NEYGDLTVRSCDPMIPPFDPGYVHTNDTVNIRLKNFIRMVNATKEYMHADEIFVRIPP
                     IYVSPQAVPTFEHYLSYIHTAFRAHGIALLGTEQALTTDSTKMCFGPNHPTNAAREEY
                     TDALIQELTAHKQLVLQ"
     gene            complement(5557..6747)
                     /locus_tag="PKZ52_06950"
     CDS             complement(5557..6747)
                     /locus_tag="PKZ52_06950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNG59547.1"
                     /translation="MDLALSYLLPAGLLCLAAWLCARFTPQYNTWLAYALFSVAPLLT
                     FTGAIAPLTGFDGAASNPWLFGCTFLTPFLALLLMTLPGQIVNHPLRFLTVCLNPIYM
                     VSGPIPTNIRIAQIRSWAIFKRRFHVYHRYAIVGAFFLFVAAPGFQKLLALKASSHPL
                     DTVLFGLSFEFYVYFNFAGYSLLAMAAMRLMGLNAPLNFRQPFSATSVVEYWQRWHAS
                     LSNVLRVLFFNPAKRRWGTWIGVCATFMASAIWHGTTINFLMWGAFQAAMWCFSRWLF
                     LNNAPKLGQYLLLISAVTIGRVIFSEVDHHILATKLGNLLTWDTVSILHAGSIILALS
                     KLDLARIGLAALVLAVEPMQALWGNPNKRYKTLRIPIFSTLMLLMVVLFSGGDLANAV
                     YGAR"
     gene            complement(7643..>7959)
                     /locus_tag="PKZ52_06955"
     CDS             complement(7643..>7959)
                     /locus_tag="PKZ52_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59548.1"
                     /translation="PWPSFATPTKIATWVLLRKNFDHDIHLKGHWDFQPPAPVAAISQ
                     TLKTQLESLPDHIVGIESTSAGVGVYWNERGGEQQLAAIYAWLDDLVSNLALQPHNRQ
                     TP"
BASE COUNT         1945 a         1793 c         2134 g         2087 t
ORIGIN      
        1 cgctgggtga gcaggtgggg attgggccgt ttaaggtctt gcagccattc gctcaggctt
       61 tcatagcgcc gttcaggatt gatatgtagg gccttttcca gggttttatc cagccagaaa
      121 ggcactaaag gattgatggc gcaggcggaa atatagttga gcttctgcag ggatttcaga
      181 tccatagcac tgctataggc actgccgtag ggcagttttt gggtgagcat ttcgtacagc
      241 agcaccccga gggagaatag gtcagagcgc tcccccgcgt ggctgcctaa acggtattcc
      301 ggtgcggagt aatccagggt gcctaaaatc gtatcgcgct caaaggtggc gccggcctct
      361 tgaatgcccg ccacccagca ggagccaaaa tcaacaatca ccgcaccgcg cttaccgatg
      421 atcacgttgt ccggttttag atcctggtgc agggtgtcct tgcggtgaaa ggcgcgcagc
      481 cctttgatga gctgatccac cagctcaatc gcatcgggga tggcaaaggg cgcgcgctct
      541 ttaatgagtt gggtgaggga tgggccctgt atgtattcgg tgaggtaata gaggcaggag
      601 cgcgggttgg gcggttccac cacgcgcacc acatgggcgt tgtggatgcg cgcgccgatc
      661 cagctttcca acacaaagcg ctcgatataa accgggtcat cctcgtaatt aactgaaggg
      721 gttttcatca ccaggggcgt gccgtcctgg gtgtgcacca gatacacctg gctgcgctcg
      781 gattcgtgca ggattttttt gatggtcaag ccatcgagaa tatgccccac ctgcaaaaat
      841 ggggggaagg gcaggcggga gagcaccgtg actgcgtcgg cctgggaggc ggtgccgcga
      901 gcctcaaccc ggcaaatttg aatgcttaag ttatcctgac ttttattggc gagcgccagt
      961 tggcagaggc cctcggcggc ggagtccaaa tcgtcgtagg cgtctttaat acactgggcg
     1021 atgagttttg cattgacgaa atcgtgcacg ccgtcggtgg taagcacaaa aatatcgcca
     1081 atctccaact cgtaggaact catgtccact tctagtaccg gatcggcgcc cagtgcacgg
     1141 gttaaatagg tggttttaac atcaactttt tgtgcatggt cgcgggtgag ttgctcaaag
     1201 ctattatccc tcaggcggta aattcggctg tcaccgacat ggaaacaaaa ggctttatcg
     1261 ccttttaaaa tcacacagga aaaggtcgtt aaatgcccct cttgtttaag cgaattttgg
     1321 cttttgccaa atagatagcg atttaagctc tgaatcacct gcaccgccga ttgctgcgtg
     1381 cgccaggtgt ctggggtggc gtagtaatcg gacaaaaaac ccgccaccgc cgtttggctc
     1441 gcctctttgc ccgccacgct gctgctcacc ccatcggcga tggccagcgc tatacccttg
     1501 gtggcgaggg ctgttccctc cggaatgcgc gcccctagag tatcttggtt ctcctgtttc
     1561 acccccgcag cagaatattg tgccaggcgc acgctaaggg tggatttaag atgctccatg
     1621 gtggcctctt aggtggcggg cggaggggga attaaaaatc cagttccggg tgaactgagt
     1681 cggagagaat acgtcgccag gcggcgacat tggccgggcc atgctggcgc agcagggtgt
     1741 tgcgaattaa ccggccaggc caggacttgc ccttgttggc cagttcaatc aagcggttgg
     1801 attcttcgac gatccaatgc acgcgaggat agcgctgttc gtggtacatc tgcagcgcat
     1861 catccaccgg aaagcgggtt attaaatccg ccaataaatt cgcatcctcc aagcccatac
     1921 ccaccccctg ctgcaaagtg ggggggcagc cgtgtaaggc atcgccaata aagaccgtgc
     1981 ggccctcaaa acagcgggtg acattgacag tctccagtgt gcccgccatc accttggtgt
     2041 caggttttag ggaatctaat aagcttttta ccggcgcgca ataatggccg aatagtgttt
     2101 gcaaggtggg aatggcgggc aactggcgcc actgatttaa agggtcggca atttgtgcat
     2161 aacagtaact ctgagtgctg cccatgggat acaccataaa ggcatcgtcg ttgcccaagt
     2221 aataaatagg gtgggcgtcc cgttcagggt gctcggcgat aaagcgccaa ttggttacgc
     2281 caaggctttt cggttttgaa tttttaaggg agagctgccg ggtggtggag tgcaggccat
     2341 cggcggccac tacaagatca aaaaattcat ggcgtgagct gtggttgatg tgcacccaca
     2401 aaccgccttc ggtttcgctc aatttagtca cttggctgct aaaacacacc gcgttggaca
     2461 taccgtattg caacagctca agcaaacgct ggcggggcag ggcgacaaac tcagcgatat
     2521 tcagcggcgg ctcgcctaag gaggcggtgg taatagtggc gccattgctc ttggcaaatt
     2581 cgattaaatt gacagtgtgg gaggcattca ttaagcggtc gtagagacct aggcggcgca
     2641 tttgaatcac agcgttggcg ggcaggcaga taccggcgcc gcccactgga aagtgggaat
     2701 ccctctcgat gatggtcact ttgacgttgg tattggtaag cctgcgcgcg agcgccaaac
     2761 cagctatacc tgcccctact atcaaaattt taatcatcgt acttaggcga tctttttcta
     2821 atgagcggaa taataacgga tttttatctg cagccgccag gtttttggct cgcaatacac
     2881 ttggcgcatg gatgcgttaa gcgttttgac ggcagtggcg ttgagtgact atagcttaag
     2941 tttgccggtg tgatcacctt gaaattccct caggttttga ttgtgtcgcg cggataattt
     3001 cactttgccg attgatgcgc tgggtggtcg gcgaatctgc ccatgtactt tttatacggg
     3061 atgagtatgc acagggttta attaggcgca aataatgatc tgcttgatga tgtccgacgg
     3121 tgtaatgggc aggccgcgag gcgctattaa agattgctaa tgaacaggcg gagcataata
     3181 ggttgggttt attattatgt tattgagtcg tttggggcgc tttccttaaa ttcattgtcc
     3241 gcatccttgg ttactttttt gtgcgattac agacatttat taacccggcg gcaaaataga
     3301 tggtcttgat ctattttgcc ttcgccgggc attctcgccg cctttattaa cccggcaaga
     3361 attgccgccg ggttaatatg ttgcatgaaa tcctgtttac aggacgaata ctcccttcca
     3421 tctgatggaa agttgaattt ttataagccg cactttgaaa aagcttagag gccccttgag
     3481 ctgattaaca cgctttcatg acccaataaa agcgtcagtt aaatgtggct gcctaacaat
     3541 atgtcttgac agcttgccgg ttagtcgccg ctaccatttt agtttttctt taatctgcgg
     3601 taataattcc tctgcaattg cttgatgggt tttggcgtct gggtggtcgt tgcaagggct
     3661 gttggggtat tttttcgacg gaatatagct gagtttggca tcatttgatg cacttacaat
     3721 agaggttaaa taggcggtta attggttttt ccgctcacca ctaataagtg gcccttcggt
     3781 gaggattatg agtgcctctg gatagagcga gcgcagttta tataacagtg tattgtaggc
     3841 actgataaat cgctccccat tgctaaggtc atcattgttg ccaagagcaa ttagaattcc
     3901 atcgggtgtg aacaaccgat gatcccagta caccgggccg ttggcggttg ttaggatgta
     3961 atcgagaaaa tgcacagcat cctgcggaat agacgccaca ctgccgttta atatgcccct
     4021 gccgcttctg cacactaact gactctcagc gtttaatgcg tgtgcgatca gggtgccgtg
     4081 ggcgacgcgg ccattgtggc tggcaggtcc tagcgcgcaa ttatccgcag gttcagaaag
     4141 cgttccgcag gttacagagg cgccaataaa cattaatttg cggttgggtt tcgttatagg
     4201 aattaacgtt tgttctgttt caagactaat taagcgtgtg gcgaaactgt cgccttcacc
     4261 tcgattgtat atctcaacca aatgggtatt tttggcactc tcgccaagat taatagatac
     4321 cgtattggtt ttatggtcga gaacataagc tttttgtatt aattgtccgt ctactaatat
     4381 atcaataaag gctcgctcga actcggcgct ggctaaaaac tctagcttag accctggggc
     4441 cataaagctt acattactgg ctgccgacgc cagtgtgata ctgtggtctg tattggttag
     4501 atagcggcct tcaatagata aactctgcgc atcaaatgag taggttgtac tttgatcggg
     4561 cttgggtggc actggtgcgg gagaattttg actattggtt gagtggtttt tgcacccact
     4621 gagattcaag agcaatacta ccaatataaa gaatgtgaaa aaaaatgaag tcattgcaat
     4681 acaagctgtt tgtgggcggt aagttcttgg ataagtgcgt cagtatactc ctcccgcgcc
     4741 gcattggtgg ggtggttcgg cccaaaacac attttagtgg agtcggttgt gagagcttgc
     4801 tctgttccca acagagcgat gccgtgtgct ctgaaggcgg tgtgtatata agacaggtaa
     4861 tgttcgaatg tggggaccgc ctgtggtgag acgtaaatag gggggatgcg aacaaatatc
     4921 tcatcagcgt gcatatattc ctttgtagca ttaaccattc ttataaagtt tttaagtctg
     4981 atgtttaccg tatcgttcgt atggacatac cccgggtcaa agggtggaat catggggtcg
     5041 caagaacgta ctgttaaatc tccatattca ttgatgctaa atgacatttt ttgtaaagcg
     5101 tttgaaatag tgcgcgctag ggggattaca ttaaatgggc gatggtatgg ttgatttgat
     5161 aatgaatcag aatatagttc gcagggagat cgtcttgcga ggtgccatat gttcatactt
     5221 gaataaatga cggatttagt ggttgagttt gattgcgaaa gccaatctgg gtaatctagt
     5281 aagccagccc cttcacaggc gctaattgca aggttgtaag catttgaatt tgttttcatt
     5341 gaaattgttc gtgcggaaac tcccgttagg gcatttgatc ctccaacgac aatatagtca
     5401 tatttgttgc cactcgtagc atttttcttt tccattgatg cgctgaacac ttcatcggtc
     5461 atcttgagga attgtatgga ttgttgcatg tagctgtaag ctaatagtag taggcacaaa
     5521 acagttttta ataggtactc gattataagc atattttcac ctggccccgt acacagcatt
     5581 ggctaaatcc ccgccggaaa ataacaccac catcaacagc atcaatgttg aaaaaatcgg
     5641 aatccttaat gttttgtaac gtttatttgg gtttccccat agcgcttgca ttggctcaac
     5701 cgccaataca agcgccgcta aaccaatgcg agctaaatca agtttgctta gcgcaaggat
     5761 aatagagcct gcatgcagta tgctgacggt atcccaagtc agcaaattgc ccaatttggt
     5821 ggctaggatg tgatgatcca cctccgagaa tatgacgcga ccgatagtaa cagcgctgat
     5881 caataacaag tactggccaa gtttaggtgc attattgagg aacagccacc gagaaaagca
     5941 ccacatggct gcctgaaaag cgccccacat cagaaagttt atggtggtgc cgtgccaaat
     6001 ggcggaggcc ataaaggtgg cgcagacacc gatccatgtg ccccagcgcc gcttggcggg
     6061 attaaaaaac aatacccgca gtacgttgct taaggaggcg tgccagcgct gccagtattc
     6121 caccacgctg gttgcgctaa agggctgcct aaagtttaag ggggcattca ggcccattag
     6181 ccgcatggcc gccatggcca acagactgta gccagcaaaa ttaaaataga cgtagaactc
     6241 gaaggataaa ccgaataaca ccgtatccag cgggtggcta gaggctttaa gggcaagtag
     6301 tttttgaaaa ccgggggcgg ctacaaatag aaagaaggcg cccactatgg cgtagcggtg
     6361 gtatacgtga aagcgccgct taaaaatcgc ccagctgcga atttgcgcta tgcgaatgtt
     6421 agtggggatg gggccgctga ccatatatat ggggttgagg cacacagtga gaaagcgtag
     6481 gggatggttg acgatctgac cgggtagggt catcagcaac agggcgagaa agggggtgag
     6541 aaaagtgcag ccaaataacc aggggttgct cgctgcccca tcaaaaccgg ttagcggggc
     6601 tatggcaccg gtgaaagtga gcagtggcgc cactgaaaag agggcgtagg ccagccaggt
     6661 gttgtactgg ggggtaaatc gagcgcagag ccaagcggcg agacacaaca aacctgcagg
     6721 taataaatat gacagagcta agtccactgt aatcttctta atgagtaact ttaacagccc
     6781 gctgaaatac tactttgcca gtacgtcgaa gcttttgtat tcactaggct gttcagcagg
     6841 atttaaaatc atccacgggc tgtgatgtgt aacataaaaa ttcatcggcc agtgttcact
     6901 ggatattcgc gcaattacct tttttatccc agtgcttgac acgtcctgtt cgtttccgag
     6961 gtgacttcaa ttgtggccat ttataatatt ccaatgcgcc tttaacgagt ctgggggaaa
     7021 ttgttttcct gcgttaattt gttgcacaga tgccgattac gtttgtgcag ttaaatcggc
     7081 agcaacgcaa acccacaatc aacgctcata aaattgctgg cggaacttaa ttaaatctaa
     7141 cagcaattac gcaagcgcct ttttcccgaa tgatttcccg aatgatgctt ccttgttcct
     7201 gcgcgatgca caaattatcg ataaatttca attgttgctg caggaaattg acacttaatt
     7261 agacttaaga agcgaacaaa aagccgcata aatgcgcgcg ttctgcccca tataatttct
     7321 tcaaacagtc gcagtttaaa ctgtcttttc catattgcat aggttttatc ggtaaatggc
     7381 aggcctattt cagcgttaat tttgctagca gaaaatatca agagtgcaat gatctcgtgg
     7441 taattatatt aacgggtggt tgatatttgc acatatttgc cgctgtgcga ctgttttgtt
     7501 tgcattttat acacctgaag gtcggtaact tgagctgtgg cgcgggtggc tattgaagct
     7561 aaaacctcag ttacacccta caacggcgtc ttcgtcacct ttgttcggag ttcagctgag
     7621 gttcttagat tgaaacttgt atttacggcg tttgccggtt gtggggttgc agtgcaaggt
     7681 tggacaccaa gtcatcaagc caggcgtaaa ttgccgccag ttgttgctcg ccgccccgtt
     7741 cattccaata aacccctacg ccagcgctgg tggattctat gcccacaata tggtccggta
     7801 gggattccag ctgggttttg agtgtttggc tgatggcggc taccggggct ggtggctgga
     7861 aatcccaatg gcccttgagg tgaatatcgt ggtcgaaatt cttgcgcagt agaacccagg
     7921 tggcgatttt tgtcggcgtg gcgaagcttg gccagggca
//
LOCUS       DAOQGS010000139         7912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4370_length_7912_cov_5.128039, whole genome shotgun sequence.
ACCESSION   DAOQGS010000139 DAOQGS010000000
VERSION     DAOQGS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4370_length_7912_cov_5.128039"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKZ52_06960"
     CDS             complement(<1..271)
                     /locus_tag="PKZ52_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59549.1"
                     /translation="MKALVLLVVALSTIKASAETVIDVFSYTKISIVSIPGTQITLHD
                     LSRTAAIEATAPEFPYDPSNPAPTVLATKTWMQSEAGQQWHRELRE"
     gene            complement(307..516)
                     /locus_tag="PKZ52_06965"
     CDS             complement(307..516)
                     /locus_tag="PKZ52_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59550.1"
                     /translation="MATIAMIKREGTWVPSVVADIRPGDVYHLIHNGVAQELRKALES
                     PRPVIINGQVIEWKIESELLNTDQW"
     gene            complement(584..3439)
                     /locus_tag="PKZ52_06970"
     CDS             complement(584..3439)
                     /locus_tag="PKZ52_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HNG59551.1"
                     /translation="MNFGKVVSDFLFRGAPSSEVVTESKARRGYLNMSSILGHLPYLD
                     TVNENSILLAQDGRSMAAVYSIKPIPTEGRSDALLEQFASNFNNFICNTFEEHATAPW
                     VVQFYAWNDKTAFYDIVGDVRNHAQHVHRSRDATPEPFTDWFIDNVFEPHIKDMMEGN
                     GLFVDSLSDQPWGGQRRRVYMVFYRLQEGAVRRRNMTPEFELENQCKKVEQMLRAAGC
                     IGSRLKGKAIRNWLFRWFNPAPKVTQGNTEALLKHYPYIPEAKRTEEFVLSADIVTNN
                     IRSDEKTKNWYFDDMPHTVLTVERLDAVPDVGQISAERYRSKEERSSASAKTVCMLDE
                     LPKGSILNLVYVVKPQQRIRKHLERLEKNSKGSTPEARAAREEVAHARMQIARGNKLY
                     PYSMNIMVRGRDDDHLDDVIQMVNTVLVSNNFKIIDSEYDLFRLDRYMRCIPCGFNPE
                     LAQLEMRNRLVYTDHLASLLPFYGRGLGTGNPGQVHFNRGGEPVCFDPTYSGDRVKNG
                     HLFLFGPTGSGKSSTLVYLLMYITAIINPRWVVIEAGNSFGLLSEFLRRMGKSTVDIV
                     LRPGKGCPSIAPFKSALQLVDEQGRVLNDVTEVEDVLAGDAELPFADDGSDESELDTN
                     SADHESIDASNRDILGELLIIARLMVTGGEANEEQRMTRADVGLLKIAILNAASQARL
                     QSKDDVLTEDVASNLKTLADERPENRKRILEMADAMQLFCSGFAGQLFNRPGEELPDA
                     DFIRIEMASLAAGNDANDKLAVAYISIINQVIARAQKTQREGRPTINLTDEAHVITTN
                     PLLAMYLVVVSKLLGRRMGLWLWQATQNMDDYKNEAKKMLAMFEWWMCLKIEAGELAK
                     IQENRSLSEDQKSMLLSMRKQTHAYTEGVVMSDTVQNLFRVVLPPLCFVLAGTELEEK
                     RARMHAMKLHKCDEVNACILMAKELSEKRRNNAIRVS"
     gene            complement(3457..3969)
                     /locus_tag="PKZ52_06975"
     CDS             complement(3457..3969)
                     /locus_tag="PKZ52_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HNG59552.1"
                     /translation="MISIPHFLSISTRLIVLAYSTLLVCGCSSHINKVTPKNLKPMDQ
                     IYDEKTGGASSEALRHRAAEIQARPAASEGMNQLPPSMQHIRELYPKLPNPDLFMYVK
                     PHVMGASGAVVPGYYTQFTLYEKTPYALPNETLGSIAPTMQRIEREQAAKEKSAAKSK
                     KHTAGDKYSH"
     gene            complement(3999..5486)
                     /locus_tag="PKZ52_06980"
     CDS             complement(3999..5486)
                     /locus_tag="PKZ52_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HNG59553.1"
                     /translation="MPTNKLILVAVGVLALAVLVAVVNKSNSPETQQAIRSEVKVKEV
                     SGDTVDETLKKTQAKMSEIRVSQSQENEAIKRELDALRLELKESEAKRAQEMNEQNER
                     NANHASELVKQRLQDFNVTMDEAARNAQSLANQFRGQSAASTDENSGKNTTDNTYKFS
                     DSDLGINGATSTAHLSGSAMPGYVALTPIQSSPGVQQRFERATSSLADSGSPTGLVSE
                     SGASKSGPAEKPKNVSKPYYTIHANSTAMDATAMTAMIGRVPIGGRAGDRFPVKFILG
                     DDTLSANGHRIPGLQGVIVSGLATGNWNLSCVSVALTSATFVFQDGRIQHLPSDVPTT
                     AVGLVTDREVGDPTAGEGQKIRESIGYISSREGIPCIAGKRITDAHKTLFTMGVLGAA
                     RSYFEGLAQAETTEIGSVENGLVGRSVTGNGKKFVNAKTAMGSVENIMDFYKDRIRDT
                     FDVVFVDPGQPVSLHFEKDLLIDYNSQARKIAYGPRDRKTAAALD"
     gene            complement(5476..6360)
                     /locus_tag="PKZ52_06985"
     CDS             complement(5476..6360)
                     /locus_tag="PKZ52_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HNG59554.1"
                     /translation="MNTLISVKNRNCLVMASLIGILTQWAQAEEERLVYHDKPMQLKL
                     PVNIEVRVKFPQDVSLQVKEDLLPKLKPLLPDAKTIYWTATEKFPASRIIATATNGSK
                     VYVFDLVADGANPAKDLVIEDPSLVSAAEQTPSVHASTANAPGADSSDATTDLPDLNA
                     ALTDPAEIVLTRFAAQTLYSPSRLLPSSDVIRQVSAPSIPPDFPLIQSRRGEQFSVKP
                     VGAWSGYGKYITAVLIVNTTSLSLQFDATAVRGNFSHITAQHINIGPKNSLEDRTTLY
                     LISDEPFGQAVLGDSYAY"
     gene            complement(6371..6967)
                     /locus_tag="PKZ52_06990"
     CDS             complement(6371..6967)
                     /locus_tag="PKZ52_06990"
                     /inference="COORDINATES: protein motif:HMM:NF022884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2895 family protein"
                     /protein_id="HNG59555.1"
                     /translation="MSEINILQNRDSHIWSLRVALFFTAIIAIFELVIIAGRDDTLTI
                     QIPPDLSKGALIKPGEFQPAHAYIFAVHMWRALNDWPTSGRNDYKSAIEVNECYVTPK
                     FAAWLVDNYAQKLRDGELERRRSVSLVDAFNDEIVKALGANTYAVYLTTHINEQIDKE
                     TIKDTKILYPLRVVADNRKCNRMGMSLDGFYEEPKRVE"
     gene            complement(6964..7305)
                     /locus_tag="PKZ52_06995"
     CDS             complement(6964..7305)
                     /locus_tag="PKZ52_06995"
                     /inference="COORDINATES: protein motif:HMM:NF023416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3487 family protein"
                     /protein_id="HNG59556.1"
                     /translation="MADALNKEPIVFRDCTGNEILLAFTYSFSLGIGLAILMGIFVMP
                     FMIALIIGLILGLTVSYAAMGFIASSRNKYYESWLNERVFTLKVTLNELFPMKTFNFI
                     NESKRYGRGRK"
     gene            complement(7351..7743)
                     /locus_tag="PKZ52_07000"
     CDS             complement(7351..7743)
                     /locus_tag="PKZ52_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59557.1"
                     /translation="MKRKTWLLKPVKTITHLYATATATLSTQLFFIANAVAADYDLPK
                     VSVPGVSSSDDPLKQGTSLMKYGIKIILWGAVIFTSVYILRNIIKEINKVRRSEEGKW
                     SDITGELLGNVMFLLGVIILGTWLNSLL"
BASE COUNT         1991 a         1902 c         1867 g         2152 t
ORIGIN      
        1 actcgcgcag ctccctatgc cattgctgcc cagcttccga ttgcatccag gttttggtcg
       61 ctaggactgt cggtgctggg tttgacgggt catagggaaa ttccggcgcg gtcgcttcga
      121 ttgcggcggt tcgagataaa tcgtgcagcg taatctgcgt gccgggtatt gagacgatag
      181 aaatctttgt atatgaaaat acatcaatta cagtctctgc agaggccttg attgtagaga
      241 gtgccacaac cagtaaaaca agtgctttca taaatcctac ctgcaagcta aaaataaacg
      301 gtttgcttac cattgatccg tattcagtag ttcactctct attttccact cgattacctg
      361 cccgttaatg ataacggggc gtggtgattc aagcgctttg cgaagctcct gggccacgcc
      421 attatggatc aagtggtaca catcacctgg gcgaatatcg gctacaacag agggcaccca
      481 ggttccctca cgtttaatca ttgcaatagt ggccatagag tgactccaga agcggcttgg
      541 tgaatagcta aataaaatca ataaaaaaat aaaaaattaa atgctaggaa actcgtatcg
      601 cattgttgcg acgcttttcc gacagttctt ttgccatcaa gatgcaggca ttaacttcat
      661 cgcatttatg cagcttcata gcgtgcatac gggcacgctt ttcctccagt tccgtcccgg
      721 ctaagacgaa acataaagga ggcagaacaa ccctaaatag gttttgaacg gtatcactca
      781 tgacgacgcc ttcggtataa gcgtgtgttt gtttgcgcat cgacaagagc atggatttct
      841 gatcttccga gagcgaccgg ttttcctgaa ttttcgcaag ttcacccgcc tcaattttta
      901 aacacatcca ccattcaaac atcgccaaca tttttttagc ttcatttttg taatcgtcca
      961 tgttctgagt ggcttgccag agccataggc ccatccgtcg ccccaacagt tttgagacaa
     1021 ctaccagata cattgctaga agtgggttgg tggtaataac gtgggcctca tcagttaaat
     1081 tgatggtggg ccgcccctcg cgttgtgttt tctgtgcccg ggcgatgact tgattgatga
     1141 tggaaatata ggcaactgcg agtttatcgt tagcatcatt tccagcggct agggatgcca
     1201 tctctatgcg gataaaatcg gcatcgggta attcttcccc gggccgatta aataattgac
     1261 ctgcaaatcc actgcaaaac aattgcattg catcagccat ttctaaaatg cgtttccgat
     1321 tttcagggcg ctcatctgcc agcgttttta aattggacgc cacatcttca gtgagcacat
     1381 catcttttga ttgaaggcgc gcttgtgagg cggcatttag aattgcgatt ttcagtaaac
     1441 cgacatccgc acgggtcatg cgttgttctt cgttggcttc accaccggtt accatcaatc
     1501 gggcgatgat aagcagctcg cctaaaatat ctcgattgga ggcgtcaata gattcatgat
     1561 cggctgaatt agtatcgagt tcggactcgt cactgccatc atcagcaaac ggcaattcgg
     1621 catcaccggc cagcacatct tcaacctcgg taacgtcgtt cagcacgcgg ccctgttcat
     1681 ccaccagctg taaagccgat ttaaatggcg cgatgctcgg gcagcctttg cctggccgca
     1741 ggacaatatc cactgtcgat ttacccattc gacgcagaaa ttcactcagt aagccgaaac
     1801 tgttgcccgc ttcaatcaca acccatcgag gattaatgat tgcggtgata tacatgagca
     1861 aatagaccag tgttgaggac ttaccggatc ccgtcggtcc aaatagaaac aggtgtccat
     1921 ttttaacgcg atcccctgaa taggtcgggt caaaacagac cggttcgccg ccgcgattaa
     1981 aatgcacctg gccaggattg cctgtaccaa ggccacggcc ataaaacggt aatagactgg
     2041 ccaggtgatc ggtgtacacg agacgattgc gcatttcgag ttgcgcgagc tcagggttaa
     2101 agccacaggg aatacagcgc atgtagcgat caaggcgaaa taaatcgtat tcgctatcaa
     2161 taatcttaaa attattgctc accaaaaccg tgttaaccat ttggataaca tcgtccaagt
     2221 gatcatcatc acggccacgc accatgatgt tcatggaata ggggtagagt ttgttgccgc
     2281 gagcaatttg catccgtgca tgggcaactt cttcgcgcgc agcgcgtgct tcaggcgtgc
     2341 tgccctttga gtttttttcc agtcgctcta ggtgtttgcg aattcgctgc tggggtttga
     2401 caacatacac cagatttaaa atgctgcctt tgggtagctc atcgagcatg cacacagttt
     2461 tagccgatgc gctagagcgc tcttcttttg atcggtagcg ctcggctgaa atctggccaa
     2521 catctggcac cgcatcaagc cgctccacgg taagaacggt gtgcggcata tcgtcgaaat
     2581 accaattctt agttttttca tcggagcgga tgttattcgt cacaatatcc gccgataaaa
     2641 caaattcctc ggttcgcttc gcttcgggaa tatagggata atgtttgagt aacgcttctg
     2701 tgttgccctg ggtgactttg ggagcaggat taaaccatcg gaataaccag ttacgtattg
     2761 ccttaccctt tagacggctg ccgatacacc ctgccgccct taacatttgt tcaaccttct
     2821 tacactggtt ttccagttca aattcagggg tcatatttcg gcgtcgaacg gcgccctcct
     2881 gcagcctata aaacaccata taaacccggc gacgctgacc accccagggc tgatcagaaa
     2941 ggctgtcaac aaatagccca ttgccctcca tcatgtcttt aatgtggggt tcaaatacat
     3001 tatcgataaa ccagtcggtg aacggctctg gggtcgcgtc tcgtgagcga tgaacatgct
     3061 gggcgtgatt acgcacatcg cccacaatgt cgtaaaacgc cgttttatca ttccaggcat
     3121 aaaactgaac gacccaggga gccgtggcat gttcttcaaa tgtgttgcag atgaaattgt
     3181 taaaattaga cgcgaattgt tctaggagcg catcagagcg tccctcggtt ggaatgggct
     3241 taatgctata aacggcagcc attgatcgcc cgtcttgggc gagcaatata ctattttcgt
     3301 ttacggtatc caggtagggt aaatggccca aaatggacga catatttaaa tagccgcgac
     3361 gcgcttttga ttcggtgacg acttcgctag agggggcacc acgaaataaa aaatcactca
     3421 ctacttttcc aaaattcata aataatcccc gtgcgtttaa tggctatatt tgtcgccagc
     3481 tgtatgcttc ttcgatttag ctgcgctttt ttctttggcg gcctgctctc gctctatacg
     3541 ttgcatagtg ggtgcgatac tgcccagggt ttcattgggc aatgcgtagg gggttttttc
     3601 atatagcgta aattgggtgt aataaccggg cacgaccgca cccgatgcac ccatgacgtg
     3661 gggtttcaca tacatgaata gatcggggtt cggtaacttg gggtagagtt cgcgaatatg
     3721 ctgcatcgag ggcggcagct gattcatacc ctcagaagcg gctgggcgcg cttgaatttc
     3781 agcggctcga tgccgcaacg cttccgagct ggcaccgcca gttttttcgt catatatctg
     3841 atccataggt tttagatttt tgggcgtaac cttgttgata tgagagctac atccgcatac
     3901 caaaagggtt gaatacgcca gtacaatcag gcgggtagaa attgataaaa aatgaggtat
     3961 agaaatcatg gtgaatactc acacgtaaaa ataatcgatt aatcaagagc cgctgcagtt
     4021 ttacgatcac gggggccgta ggctattttt cgcgcttggg agttgtaatc aataagtaga
     4081 tctttttcaa aatgcagtga caccggttgg cctgggtcaa caaaaaccac atcaaacgtg
     4141 tctcgaatgc ggtccttata gaaatccata atattttcta ctgaacccat tgctgtttta
     4201 gcattaacga attttttgcc gttgcccgtc acggatcgac ctacaaggcc attttctacg
     4261 ctgccaattt cggtggtttc tgcctgcgct aagccttcaa aataactgcg cgcagcaccc
     4321 agtacaccca tcgtaaatag cgttttatgg gcatcggtaa ttcgtttgcc ggcaatacac
     4381 gggatgcctt cgcgtgagga tatgtagcct atggactcac ggattttttg accctcaccg
     4441 gcggtgggat ctcccacctc ccgatcagtc actaagccca cggcggtggt gggcacgtcg
     4501 gaggggagat gctgaatacg tccgtcttgg aacacgaagg tggcactggt cagtgctacc
     4561 gagacacagg acagattcca gttgccagtg gcaagccctg atacgattac gccctgcagg
     4621 ccaggaatgc ggtgtccatt tgcggatagg gtgtcatcac ccaggatgaa tttcacagga
     4681 aagcgatcac ctgcacgtcc gccaatgggg actcgcccga tcatggcggt catcgcggtg
     4741 gcatccatgg ccgtactatt ggcgtgaatg gtgtagtaag gcttggatac gtttttcggc
     4801 ttttctgcag ggccgctttt cgacgcaccg gattcactaa ctaacccagt agggctgcct
     4861 gaatcggcta atgaagacgt tgcgcgttca aaccgttgct gaacacctgg acttgactga
     4921 atcggcgtca acgccacgta gcctggcata gcactacctg ataagtgagc cgtgctggtt
     4981 gccccattaa ttcctaaatc ggaatcggaa aacttatagg tattatcagt cgtgtttttt
     5041 cctgaatttt cgtcagtcga tgctgctgat tggccacgaa attggtttgc cagagattgg
     5101 gcattgcgag ccgcctcatc catggtcacg ttaaaatcct gcaggcgttg ttttaccaat
     5161 tctgatgcgt gattagcatt gcgttcattt tgctcattca tttcctgcgc gcgtttcgct
     5221 tcggattctt taagctctaa gcgtaaggca tccaattcac gttttatagc ctcattttcc
     5281 tggctttgac tcacgcgtat ttcggacatt tttgcctgag tcttttttaa tgtttcatcg
     5341 acggtatcgc cggagacttc tttaactttg acttcagatc gaatggcttg ctgtgtttcc
     5401 ggtgaattcg atttattgac aaccgccacc agtaccgcca gggccagcac acccacggcc
     5461 accagaatga gtttattagt aggcataact gtcccccaaa actgcttgac caaagggttc
     5521 atcgctaatg agatacagtg tggtgcgatc ttctaaactg ttttttggcc ctatgttgat
     5581 atgttgcgcg gtaatgtggg agaaattacc ccgcacggcc gttgcatcaa attgcagaga
     5641 cagtgaggtc gtatttacga taagaacggc ggtaatatat tttccataac ccgaccaagc
     5701 gccaacgggt ttaacagaga attgttcacc tctgcggctt tggatcaatg ggaagtctgg
     5761 cggaatactc ggcgcgctca cttggcgaat aacgtcactg gacggcaata accgcgaggg
     5821 tgaatacagt gtttgtgcgg caaatcgagt taaaacaatt tcggcaggat cggtcagggc
     5881 agcattcaga tccggcaggt ctgttgtggc atctgaagaa tctgcccctg gcgcattagc
     5941 cgtactcgcg tgaacggacg gagtttgttc agccgccgat accagcgatg ggtcttcaat
     6001 caccaaatct ttcgctgggt ttgctccatc ggctacaagg tcaaacacat agaccttgga
     6061 tccattcgtt gcggttgcaa taatgcgcga cgcaggaaac ttttcggttg ccgtccaata
     6121 aatggttttt gcatctggca ataaaggttt taacttgggt aaaagatctt cttttacctg
     6181 caagctcacg tcctggggaa atttaactct cacctcaata ttgaccggta atttgagctg
     6241 catgggtttg tcgtgataca ccagtcgctc ttcttcggcc tgtgcccatt gagttaaaat
     6301 gccaattagt gacgccatta caaggcaatt tcgattttta actgaaatca gtgtgttcat
     6361 taggcctgcc tcattcgact cgttttggtt cttcatagaa tccatccagg gacataccca
     6421 tgcgattgca ttttcgatta tctgcaacca cgcgcagtgg atagagtatt ttggtgtcct
     6481 taatagtttc tttatcgatt tgctcgttga tatgcgtcgt taaatagacg gcataggtat
     6541 ttgcgcccag ggccttaacg atttcgtcgt tgaacgcatc cactaggctc acgctgcgtc
     6601 tgcgctctaa ctcaccgtct cgaagttttt gcgcatagtt atctaccaac caggccgcaa
     6661 attttggcgt gacataacat tcgttaacct ctatcgcgga tttgtagtca tttctcccag
     6721 aggtaggcca atcgttcagt gctcgccaca tatggacagc aaatatataa gcgtgggcag
     6781 gctggaactc gccgggtttt attaatgcac cttttgataa atcaggcggg atttgaatgg
     6841 ttagggtatc gtcgcggcct gcaatgatca ccagctcaaa aatagcgata atagctgtaa
     6901 aaaacagcgc caccctcagt gaccaaatgt ggctatcgcg attttgtaaa atattaattt
     6961 cactcatttt cggcctcggc catagcgttt tgattcgttt ataaagttaa aggtcttcat
     7021 gggaaatagt tcattcagag tgactttgag tgtaaacacg cgctcattaa gccaggactc
     7081 ataatattta ttgcgtgaag acgctataaa tcccatggcg gcataactca cggttaggcc
     7141 taatatgaga ccaatgatca gtgcgatcat aaagggcatc acgaagatgc ccatgagtat
     7201 ggcgaggccg atacctagcg aaaaagaata ggtaaatgcc agcaaaatct cattgcctgt
     7261 acagtcacga aacacaatag gctctttatt aagtgcgtcc gccaatcgca ttccacttcg
     7321 attctctccg ctcatatctg cctcagatgc ttatagaagt gagttcaacc aagtgccgag
     7381 gatgattacc cccaacagaa acatgacgtt gcccaacaat tcaccggtaa tatccgacca
     7441 cttaccctct tcggatcgac ggactttatt tatttcctta ataatgtttc gcagaatgta
     7501 aacagacgta aaaattacgg caccccagag tattattttg atgccgtatt tcatcaggct
     7561 tgtaccctgt ttcaatgggt catctgaact tgataccccc ggaacgctga ccttaggtag
     7621 gtcatagtcc gcagctacag cattagcaat aaaaaatagc tgcgtgctga gggtcgccgt
     7681 agcggttgca tataaatggg ttattgtttt tactggcttt aatagccatg ttttacgctt
     7741 cataacgtca ctcctagcgg atattgaaca acaaggaaaa taggagcatt aggaataatg
     7801 cccgtatcgt gacggtaaaa aagtcgaaat gagagaaatc gttaagtcgt atgcggtcac
     7861 gccaaccttt gcgcatcacc acgatcaatc caattcccag aaaacaaacc ag
//
LOCUS       DAOQGS010000140         7806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4463_length_7806_cov_5.266933, whole genome shotgun sequence.
ACCESSION   DAOQGS010000140 DAOQGS010000000
VERSION     DAOQGS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_7806_cov_5.266933"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PKZ52_07005"
     CDS             <1..1761
                     /locus_tag="PKZ52_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNG59558.1"
                     /translation="AWADNQFKTLSFLNSIRGVSTLGGLHNRQPNGDPNLYSRQLNAA
                     TGLWPGLYSADFLFEGVEIANRQKMIDQVKVEWSHGAAINLMWHACNPAKSEPCEYSK
                     AHGTGPWSDMSDAEWAQLMTEGSAINTEWKRRMDKVAVFLAQLQQLNIEVMFRPLHEM
                     NQRDFWWGGRPGPNGTAKLYRMTHDYFVNTKGLKNLIWVWDVQDFGSLASDLNTYDPG
                     SNYWDVLALDVYRSDGQGFSQQKYNLIANKAAGKPIGIGECDVLPSLAELNAQPLWSF
                     FMGWSELVFKENDAQKINNVYGAGRVLTLNELPGWNTVAVPGVNPNPAPTFNGITVQA
                     EDYANMSGVAIEDTSDIAGGLDVGAIDTGDWLSFSGINIPAAGNYTVSFRVASPVDTG
                     RLQFEQAGAGKIYGAPFAIPNTGDWQNWTTISQVIWLDKGLQDFGIKALAGDWNINWF
                     SITPLPTATVIQAENFTNMSGVVPEPTSDTGGGIDIGGIDTGDWLSFSRISVPVTGTY
                     TLEFRVASPTGGRIQFEQAGGGIIYGTANIPATGGWQNWQSVKISVNLSAGVQDFGIK
                     AVQGNWNFNWWSLTPLTATH"
     gene            2274..2939
                     /locus_tag="PKZ52_07010"
     CDS             2274..2939
                     /locus_tag="PKZ52_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HNG59559.1"
                     /translation="MFSASTFAASYKVEIQNLTQGIYFAPLLVATHNNEVSLFQAGMP
                     APANLQKLAEGADNSGFISELTAAGATPIQNPAGGLLAPGAKVMVDLGMPDEQHRQLS
                     IIGMIVPSNDGFVALHKLVLPSTPGTYTYMVNAYDAGTEANDEIRGGGAPGVAGLPVP
                     PPLANKVGTGGTGIVGASVEGFVHIHRGVIGDTTMLGGTSDLNAATHRWLNPVGRITL
                     TVE"
     gene            2950..3795
                     /locus_tag="PKZ52_07015"
     CDS             2950..3795
                     /locus_tag="PKZ52_07015"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HNG59560.1"
                     /translation="MNIKVLISTLLGALILAGCTGGKAHKSASSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSGASVVLAHYRLKIINLTAGQPLSPPAFVVHNAEY
                     SAFAIGAEASLGIEKIAESGNAADFIAAAKNHAGVFLADRGASGIMPGHEQEIDLMVE
                     LPGAQLSGLKLSYLSMLGNTNDGFAGVDNLALGQLSVGATISRDALSYDAGTELNDEL
                     ATTIPGPACGGEGFNPLRSDGVNQITLHPGIVSQHDGNPNSCLQTLQRWDNPVARVMI
                     TRLAL"
     gene            4319..5047
                     /locus_tag="PKZ52_07020"
     CDS             4319..5047
                     /locus_tag="PKZ52_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG59561.1"
                     /translation="MTVSQFPPIKKRRFLIVEDELDLAKLLVLHLQDFNADITCIDRG
                     DVALQVAQKEPWDLLILDLRLPGLDGLEVCRQLRAKAVEVPLLMLTARSAELDRVLGL
                     ELGADDYLTKPFSAMELVARVKALLRRAGRHMAPPAATELLRFKHMSIDRGARSVTLG
                     GKVIELTAREFDLLWFFASNPGRVFRRGELLDSVWGYGHEGYEHTVNSHINRLRAKIE
                     ADAANPEVLLTVWGVGYKFNEECA"
     gene            5047..6576
                     /locus_tag="PKZ52_07025"
     CDS             5047..6576
                     /locus_tag="PKZ52_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG59562.1"
                     /translation="MVVGLHSLYGRLAAVFVCLLCILGAGVLALTNWSHNLYYQEVTQ
                     KLNKSLAMYIVQRAPLIEQGQVNKNRVKELADLAMVVNPIVEVYVLDPQGQILAHSLP
                     PETILHTRIDVRPLQQWLAGEQNNMVRAQDPRAQDELRVFSAFPIESNAHLEGYLYVV
                     LGGQAYQSLSQSLKGSFILKQSVFSLIALLIFATLCAALLFAWLTRPLRQLTARINQF
                     GRRQAWLSQAVVPNHNEVEYISQAFDSMAQRIEQQIAQLETTDHLRRELISNVSHDLR
                     TPIALMQGYLELLLKPGTTAAEHSQYVGIALKHCEQLTQLVKELFELSKLDAGRIQPE
                     YERFALAELLQDVAQKFALKAVEHSIELKMDMQGGAFMVFADISLIERVLENLIDNAL
                     RYTPNGGEVVLRLVNKVDSVEVEIADTGIGLAAQDIPFIFERYYCANKPTVYSKNSTG
                     LGLAIVKKILDLHHSSIAVESQVNKGSVFRFPLPNNERTIKWRSAPGAKGDLQRALTA
                     C"
     gene            7096..>7806
                     /locus_tag="PKZ52_07030"
     CDS             7096..>7806
                     /locus_tag="PKZ52_07030"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malectin"
                     /protein_id="HNG59563.1"
                     /translation="MPLDRSYDSSAVPQWLNPTLKQLLRLCRWVWCPLVIGYSLALTS
                     CGATDLGASSSSSSSSSSSSSSSGSTSSSSSSSGHSSSSSSSSGAINPGKLVVAINAG
                     ASEAAVFNKEEYGPDRFFSGGTTQVVDRPINGVDEDVIFQSERYGNVQYEIPLSNATY
                     TVVLHFAELYQQAEAARTFNVLVENKPALTGLDLFKQVGRDTAFSYRLDNISVTDGSL
                     SIALNTLTDNATISAIAVY"
BASE COUNT         1910 a         1831 c         1998 g         2067 t
ORIGIN      
        1 gcctgggcag ataatcaatt taaaacgctt agttttttaa atagcatacg cggggttagt
       61 actttgggtg gtttgcacaa ccggcagccc aatggcgacc ctaacttgta cagtcgtcaa
      121 ctcaatgccg caaccgggct ttggcccggc ctttacagtg ctgatttttt atttgagggt
      181 gttgagattg ccaatcggca aaaaatgatt gaccaggtta aagtggagtg gagccacggt
      241 gcggcaatca atttaatgtg gcatgcatgt aacccggcta aaagtgaacc ctgtgagtac
      301 tctaaggccc acggtactgg cccttggagt gatatgagcg atgcagaatg ggcgcaactt
      361 atgacggagg gttcggcgat aaataccgag tggaagcgcc gcatggataa ggttgcggta
      421 tttctagcgc aattacagca gctcaatatt gaagtgatgt ttcggcccct gcacgaaatg
      481 aatcaacggg atttttggtg gggtggcagg cccggcccaa acggcacggc aaaattgtac
      541 cgcatgaccc acgactattt tgtcaacacc aaaggcctta aaaatttaat ttgggtctgg
      601 gatgtacagg attttggctc cctcgccagc gatttaaata cctacgatcc aggcagtaat
      661 tattgggatg tgctcgcctt ggatgtatac cggtcagatg gccaaggctt cagtcagcag
      721 aagtataatt taatcgccaa taaagcagct ggaaaaccca ttggcatagg tgagtgcgat
      781 gtgttgccaa gcctggcaga attaaatgcg cagcccttat ggtcgttttt tatggggtgg
      841 tctgaattgg tatttaaaga aaacgatgcg caaaaaatta acaatgtgta tggcgctggc
      901 cgcgtattaa cactcaatga attgcccggc tggaatactg ttgctgtgcc cggtgtaaat
      961 cctaaccccg cccctacttt taatggcatt actgtccagg cagaggacta tgccaatatg
     1021 agcggtgttg ctattgagga tactagcgat attgccggtg gccttgatgt gggcgctata
     1081 gatacagggg attggttatc gttcagcggc attaatattc ctgccgcagg taattacact
     1141 gtgtctttcc gagtggcaag cccggttgat acagggcggt tgcaatttga gcaagcaggg
     1201 gctggcaaaa tatacggcgc gccgtttgcc atacccaata ctggggactg gcaaaattgg
     1261 acgactattt cccaggtgat ttggctggat aaaggcctgc aagactttgg cattaaggca
     1321 ttggcgggtg attggaatat taattggttt agtattacgc ccctgccgac cgccactgtt
     1381 atccaggcgg aaaacttcac caatatgagc ggcgtagtgc cggagccaac cagtgatact
     1441 ggcggcggta ttgatattgg tggcatagat accggcgact ggctttcctt tagcagaata
     1501 tctgtgcccg tgacgggcac ttatacgctg gaatttagag tggcaagccc aacgggtggg
     1561 cgcatccaat ttgaacaggc cggcggcgga attatttatg gtaccgcgaa tattcccgca
     1621 acaggcggct ggcaaaattg gcaatcggtt aaaataagcg ttaatttgtc ggccggggtg
     1681 caagattttg gcattaaggc ggttcaaggc aactggaatt ttaattggtg gtctcttact
     1741 cccttaacgg ctacacatta aatccccggt tatttctaaa agcataatta cacgttgtta
     1801 attttcccgc caaagggtgc aataggaatc tattgcaccc tttttgccgc tgtgagtatt
     1861 tatgcggctt ttttttggaa tgggtgggcg gtgcccacgg cccaatcata gccttacatg
     1921 ctaatcgata tttcacttta tgcagtgcat aaaattcccg cattgtgctg cgctccattc
     1981 aggctacaga aaacaggtat tcattcaatg aatggcgtag ccttggctgg ggcgcacgaa
     2041 gggcgctgcg gctgtttttg taatctgctg ccccttcatc gcgttggaaa tgttagcgcg
     2101 taggtaattc aatacagtgc gtccatcccc cgcgataaaa acacaacact attgtgatac
     2161 ttctgtgaag aattagcccc ccgcagcgga gcattctgtg ggtgcgttaa cccgactcac
     2221 acaggatatt aattatgaaa attctaccca gtttactttg tgcaggcgcc tgtatgttca
     2281 gcgcttctac cttcgctgca tcatataaag ttgaaattca aaaccttacc caagggattt
     2341 attttgcgcc tctattagtg gccactcata acaacgaggt ttccttattt caagccggta
     2401 tgcctgctcc tgccaattta caaaaattag ccgaaggggc ggataacagc ggatttataa
     2461 gcgagctaac tgccgcaggt gccacgccga tccaaaaccc tgccggtggc ctattggcgc
     2521 cgggtgcaaa agtgatggtg gatttaggca tgccggatga acagcatcgg caactcagta
     2581 ttatcggcat gattgtgccg agcaacgacg gttttgtcgc tttacataaa cttgttttac
     2641 ccagcacacc cggcacttac acctatatgg taaatgccta tgatgcgggc accgaggcca
     2701 atgatgaaat ccgcggtggc ggtgcaccgg gtgtggcggg tttgccagtg ccaccgccgc
     2761 tagccaataa agtgggcact ggcggcacag gtattgttgg cgcaagtgtc gagggatttg
     2821 tgcatattca ccgaggggtc ataggcgaca caaccatgct gggcggcact agtgatttaa
     2881 atgcggctac ccaccgctgg ctaaatccag ttggccgcat caccttaacc gtggaataag
     2941 gagctattta tgaacattaa agtattaata tccacgctcc tgggtgcgct aattttagct
     3001 ggatgtactg gcggcaaagc gcataaatcc gctagctcat caagtagttc atcttccagt
     3061 tcaagttctt cttctagttc aagtagttct tctagttcaa gtagttcttc tagttcaagt
     3121 agttcgtcgt cgagttctag ctcatcctcc agtggcgcca gtgtggtttt agcccattac
     3181 aggttaaaaa ttattaattt aaccgctggg caaccgctat caccaccggc ttttgttgtg
     3241 cataacgctg aatattcagc ctttgccatc ggtgctgagg caagcctggg gatagaaaaa
     3301 atcgccgagt cgggcaatgc tgcggatttt atcgccgctg ccaaaaatca cgctggagta
     3361 tttttggcgg accgaggtgc cagtggcatt atgccgggcc acgagcagga aattgaccta
     3421 atggttgaac taccgggtgc gcagctgagt gggctcaaat tgagttattt atccatgctg
     3481 ggcaacacca atgacggttt tgcaggcgtt gataatctgg ccctggggca gctctcggta
     3541 ggcgccacta ttagccgcga cgctctttcc tatgatgccg gtacagaact gaatgatgaa
     3601 ttggcaacga caattcctgg ccctgcctgt ggcggtgaag gatttaatcc actacgcagc
     3661 gacggggtaa atcaaataac cctgcaccca ggaattgtca gccaacacga tggcaacccc
     3721 aattcgtgtt tgcaaacact gcagcggtgg gataatccgg tggctcgggt tatgatcacg
     3781 cgattggcgc tttaggcatg catttagggc cggattttta attgtttctg cgcttatcca
     3841 aaaactcctc cccttgtaaa gaggagaggg agcaaaatac cgtcatgtta aggtttttgc
     3901 tcctccctct tattcaggga gaggctgggt gggtaggcat aaagcagagc gccatttaac
     3961 gcaagcagaa tgcatcacct tattacaaag ggtgcgctgc attcagcatt aatcttgagc
     4021 tgcacggctg tttaaaagcg ctaaggaacc tctgaaaaat taccttactc gctctggcgt
     4081 cgttgaactt gaaaaaaaat tctcagttac tgcgtgtgaa ctcggcaatc gctcctgcat
     4141 tgctctacct tctgcatcct tgcagtcgtc cgcttatttt ggcaatttcg cctggccagc
     4201 ggttcgccgc cgacattttt cagaggttcc ctaaaactat ccacctgttt tttaaaaaga
     4261 acttagttga gcaaattgtt atctgctttt aacgaagcaa attcatcggg atcaaaccat
     4321 gactgtcagc caattcccac ccattaaaaa acgccgcttt ctaatcgttg aagacgaatt
     4381 ggatttagcc aaattattgg ttttgcattt gcaggatttt aacgcggaca tcacctgcat
     4441 cgaccgggga gatgtggccc tgcaggtggc gcaaaaagaa ccctgggatt tattaatttt
     4501 ggatttacgc ctgccgggcc tggatggttt ggaagtgtgt cggcagttgc gcgcgaaagc
     4561 ggtggaagtg ccgctgctga tgttaacggc gcgttcggct gagttggacc gggttttagg
     4621 ccttgaatta ggtgccgatg attatttaac caaacccttt agcgctatgg aattggtggc
     4681 ccgagttaaa gcgctactgc gccgtgccgg gcgccacatg gcgccgcctg cggcaaccga
     4741 attattgcgc tttaaacata tgtcgattga tcgcggcgcg cgcagcgtaa ccctgggcgg
     4801 taaagtcatt gaattaaccg cgcgggaatt tgatctctta tggttttttg cctctaaccc
     4861 agggcgggta tttcgccggg gtgaattatt agattccgtg tggggttatg gccatgaggg
     4921 ttatgaacac acagtcaata gccatataaa ccgtctgcgg gcaaaaattg aagccgatgc
     4981 ggccaacccg gaagttttac tcaccgtttg gggagtaggc tataaattta atgaggagtg
     5041 cgcttgatgg tggtgggttt gcattctttg tacggccgcc tagcagcggt ttttgtgtgt
     5101 ttactgtgta ttttaggtgc gggcgttttg gcgcttacca attggagcca caatctttat
     5161 taccaggagg ttacgcaaaa actcaacaaa agtttagcca tgtatatagt gcagcgtgcg
     5221 ccattaattg agcaggggca ggtaaataaa aatcgggtaa aagaattggc cgatttggca
     5281 atggtggtga accctattgt tgaggtttat gtgttagatc cacagggcca aatattggcc
     5341 cattccctgc cgccggaaac tattttgcac acacgcattg acgtgcgccc gctgcagcag
     5401 tggttggcag gtgagcagaa taatatggtg cgcgcccaag acccgcgcgc ccaggatgag
     5461 ctgcgggtat tttcagcctt tcctattgag tcaaatgcac acttagaagg ctacctatat
     5521 gtagtattgg gcgggcaagc ttatcaaagc ctcagccaat cgcttaaggg cagttttatt
     5581 ttaaaacaga gtgtgttttc gcttattgcg ctactgattt ttgccacttt gtgtgcggcg
     5641 cttttatttg cttggctcac ccgcccactg cggcaattaa cggccagaat aaatcagttt
     5701 ggccgcaggc aggcctggtt gtcccaagcc gtggtgccaa accacaatga agtggaatac
     5761 atatcccaag cgtttgattc tatggcgcag cgcatagagc aacaaattgc tcagctggaa
     5821 accaccgatc acttgcggcg ggaattaatc tccaacgtct cccatgattt acgcacgcct
     5881 attgcactta tgcaggggta tcttgaattg ctgcttaaac ccggcactac agcggctgag
     5941 cacagccaat atgtgggcat agcgcttaaa cactgcgagc agttaactca attggtgaag
     6001 gaattattcg agctgagcaa attagacgcc gggcgcattc agccggaata cgagcgtttt
     6061 gcgcttgcgg agttactgca agacgtagcc cagaaatttg ccctaaaggc cgtggagcac
     6121 agtattgaat taaaaatgga tatgcagggc ggtgcgttta tggtatttgc cgatatatct
     6181 ttaattgagc gggtgctgga gaatttaatc gataacgccc tgcgctacac tcccaacggc
     6241 ggcgaagtcg tgctgcggtt agtgaacaaa gtggactccg tggaggtgga aattgccgat
     6301 acgggcatcg gcctggccgc gcaggatatt ccatttattt ttgagcgtta ttattgtgcg
     6361 aataaaccga cggtttatag caaaaatagc acggggcttg gcctggctat tgttaaaaaa
     6421 atcctcgatc tgcaccatag ttccatcgcg gtggaatccc aggtgaataa aggcagtgta
     6481 tttcgattcc cgctgccaaa taacgagcgc actattaaat ggcgttcggc gccgggggca
     6541 aaaggggatt tacagcgcgc cctgaccgcc tgttgaacta ctacatcacc aattattttt
     6601 gattgatccc gcatgagtaa tagcgcctcc ttcctgccta atgcttgttc ctgcacttct
     6661 attaacccgg cggctaaata gatcgtcctg atctatttaa ccttcgctgc cctaaaactg
     6721 gcgaaaatgc gtggatttac ttgttttagg gcagctcacg gcggtttgca cctttggagc
     6781 ggcatccatg ccgccattat taacccggca ctaaatgccg ccgggttaat aactcgaggc
     6841 ctgccattag gttttggcct caaaacctga gctaattggc agcccaaatg cgacttgcct
     6901 aacgctttat ccaatccatt ccctgccact tatcaaattg cgacggacat agcgctttta
     6961 tcttctattc actaagacaa ttacaaaagg tcagacaata taactattaa gtgccccggt
     7021 tgataggttt ggcccagtct tgagccgctt tttcatttgc ctcacccaaa ttatctaaaa
     7081 ataaaaggcc cacctatgcc ccttgatcgc tcttatgatt cgtccgcagt gccgcaatgg
     7141 cttaacccaa cacttaagca gttgttacgc ctctgccgtt gggtttggtg cccgctcgtt
     7201 attggttata gcttggcgct caccagttgc ggggccacag acctaggggc cagcagttca
     7261 tcctcaagct cgtcgtccag tagctcatcc agctcgggaa gcacaagcag ttcaagttcc
     7321 tcatccggcc acagcagttc ttcctccagc tccagtgggg ctataaaccc aggcaaattg
     7381 gtggtggcca ttaatgcagg cgccagcgaa gcggcggtat ttaataaaga agaatacggg
     7441 ccggatcgtt tttttagcgg cggcaccaca caggtagttg accggcccat taatggcgtg
     7501 gatgaagatg taattttcca atctgagcgc tatggcaatg tgcagtacga aattcccctt
     7561 agcaatgcta cttacacagt ggtgctgcat tttgctgaac tctaccagca ggccgaggcc
     7621 gcccgtacct ttaatgtatt agtggaaaat aaacctgcat taaccgggct ggatttattt
     7681 aaacaggtgg gccgcgatac ggcctttagt tatcgtctag ataatattag cgttacagac
     7741 ggctccctct ctattgcact taataccctc accgataatg ccaccatcag cgctattgcg
     7801 gtttat
//
LOCUS       DAOQGS010000141         7791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4477_length_7791_cov_4.270683, whole genome shotgun sequence.
ACCESSION   DAOQGS010000141 DAOQGS010000000
VERSION     DAOQGS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_7791_cov_4.270683"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PKZ52_07035"
     CDS             <1..1630
                     /locus_tag="PKZ52_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNG59564.1"
                     /translation="LLIFPQILYLLGPVGGGKSSLAEKLKALMEKQPIYCLKGSPVFE
                     SPLGLFDPNEDGVILQEDYGIPLRYLKSVMSPWAVKRLHEFGGDISQFKVVKVYPSIL
                     DQVAISKTEPGDENNQDISSLVGKVDIRKLEEFPQNDPDAYSFSGGLCRANQGIMEFV
                     EMFKAPIKVLHPLLTATQEGNYNSTEGLGAIPFDGTILAHSNESEWQTFRNNKNNEAF
                     IDRVYIVKVPYCLRVSEEIHIYEKLLQNSSLRRAPCAPDTLKMLAQFAVLSRLKVPEN
                     SNLFSKMRVYDGENLKDTDPKAKSLQEYKDAAGVDEGMNGLSTRFAFKILSKVFNFDP
                     TEVAANPVHLLYVLEQQIEQEQFPEERAGRYLAFLKEYLAPRYVEFIGKEIQTAYLES
                     YAEYGQNIFDRYVVYADFWIQDQEYRDPETGEILNRAAINEELEKIEKPAGISNPKDF
                     RNEIVNFVLRARANNNGKNPVWNSYEKLRVVIEKKMFSNTEDLLPVISFNTKSSKEDQ
                     KKHQDFVARMVERGYTEKQVRLLSEWYLRVRKSQ"
     gene            1773..3050
                     /locus_tag="PKZ52_07040"
     CDS             1773..3050
                     /locus_tag="PKZ52_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNG59565.1"
                     /translation="MSYVVDRRLNAKNKSTVNRQRFLDRYRDHIKKAVQQAVDKRSIK
                     DIESGEQISIPSRDISEPTFHHGQGGVRNRVLPGNKEFVSGDKIQRPEGGGGGSGSGK
                     GEASDSGEGEDEFVFQITQEEFLNYMFEDLELPNLVKRQLAGGEEFKYHRAGIANEGN
                     PGKVNIVRSLRAANARRMALTGQKRKQLRAYEEELAQLTEGDDPLVAARRKELEQLIV
                     GLKARIKRVPWLDDFDLKYNLQVKHPVPSSKAVMFCVMDVSGSMDQATKDVAKRFFLL
                     LYLFLQRSYGHTEVVFIRHHTSAKEVDEQEFFYSRETGGTIVSSALRLMDEIIDARFP
                     TSEWNIYCAQASDGDNWNDDSSVCYKMLVEHIMQKVQYFSYIEITPRDHQALWRSYEQ
                     VEESFPDSFAMRQIQSLADIYPVFRDLFQKKAA"
     gene            3053..4561
                     /locus_tag="PKZ52_07045"
     CDS             3053..4561
                     /locus_tag="PKZ52_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNG59566.1"
                     /translation="MEKHPISTGSEWSFELIEAYDKAIAEIAKEYRLDTYPNQIEIIS
                     SEQMMDAYASVGMPLGYNHWSYGKQFLNVEKSYRRGQMGLAYEIVINSNPCIAYLMEE
                     NTMMMQALVIAHACYGHNSFFKGNYLFRAWTDASSIIDYLVFAKNYISQCEEKHGVAA
                     VEAILDSCHAIMNYGVDRYKRPYPISAHMEQARQKEREEYLQRQVNDLWRTIPADEQK
                     KNQQKQFRFPEEPQENLLYFFEKHAPLLEPWQREVVRIVRKIAQYFYPQRQTQVMNEG
                     WATFWHYTLINTLFERGLVTEGFMLEFMQSHTSVIYQPPYHAKYYNGINPYALGFAMM
                     QDIRRICEHPTDEDRQWFPDIAGSDWVATLDFAMRNFKDESFILQFLSPKVMRDLKLF
                     TILDDDRNSTIDVTAIHNDSGYKAVREALSAQYNLGNREPNIQVYSVDVRGDRSLTLQ
                     HIQHNRQPLDASAQEVLKHIHRLWGFDVRLDSICDGAVAQSFRCSSNLAEPV"
     assembly_gap    4726..4735
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4736..5402
                     /locus_tag="PKZ52_07050"
     CDS             <4736..5402
                     /locus_tag="PKZ52_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG59567.1"
                     /translation="AICVNYNSSKQAAASLCERISALGARVIAVKADVSLERDVLQLF
                     KEIDSQLGVVTHLVNNAGVIAPIKKFEATTLDDLQRTFSVNVFGSFLCAREAVKRMCT
                     LSGGKGGVIVNMPSVASRIGSPNEFIEYAASKGAIDTFTLGLSKELANRGIRVNAVRP
                     GLIDTEIHAHAGDAARPERLKSAIPMQRIGTACEVAEAVMWLLSDSASYVTGTILDVA
                     GGR"
     gene            complement(5485..6039)
                     /gene="orn"
                     /locus_tag="PKZ52_07055"
     CDS             complement(5485..6039)
                     /gene="orn"
                     /locus_tag="PKZ52_07055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNG59568.1"
                     /translation="MPQPSPCDSHLIWIDLEMTGLNPEADVIIEIATLVTDKDLNILA
                     EGPVFAINQHPKTLAAMDAWNTQQHGGSGLVQRVLDSRTTTAQAEQATVEFLQQWVPA
                     GKSPMCGNSICQDRRFLAHYMPQLEAFFSYRNLDVSTLKELAKRWKPEISKGLTKKGS
                     HLALDDIRDSVAELRYYREHFIKL"
     gene            6129..7109
                     /gene="rsgA"
                     /locus_tag="PKZ52_07060"
     CDS             6129..7109
                     /gene="rsgA"
                     /locus_tag="PKZ52_07060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HNG59569.1"
                     /translation="MSKRRLSLQQTRRIAQQNNQGVEGDVTGCEGVVISPLRQQAEIE
                     DSATGERYRCHLRANLPPLTVGDRVVWQPGSPTGVVVALKPRRNCIQRPDSFGNLKLV
                     AANVDCALIVLAPEPVAHANLIDRYLVALHALDIRPVLVLNKSDLLVPDHPMLALAAS
                     YEALGYTCLKVSAQAANSLEPLQRVLAEGTSILVGQSGVGKSSLLQALLPDEQIKIGE
                     LSEGVAKGRHTTTHARLYHFAGGGACIDSPGIREFGLGHLSPEQVAQGFVEFEGFLGQ
                     CKFRNCLHQQDPGCALLHAREEGVISSERFASYQQILASQSLTTPKTSTP"
     gene            7106..>7791
                     /locus_tag="PKZ52_07065"
     CDS             7106..>7791
                     /locus_tag="PKZ52_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG59570.1"
                     /translation="MTLSLSTARASLAAWVLEPAEAERLPPETLILDCSSQAQYEAGH
                     WPGALWLPPQFLLCGTAPVPGKPPHISELNRLFDCLGLYPERPVLVMDDEGGGWAGRL
                     LWTLDLIGHSPAYYLNGGVRALLSDGIALEQTIHQPQAISPIPRQLQPACRVELDQLL
                     GRYSSTQIWDARSLGEYQGWLRAATYTGHIPGARHLEWSELYDPARGYRIREDALGRI
                     IEAGLDPARP"
BASE COUNT         1851 a         1771 c         2139 g         2020 t
ORIGIN      
        1 gcttttaatt tttccgcaaa ttctgtattt gttggggccg gttggcggcg gtaaatcctc
       61 attggcggaa aaattaaaag ccctgatgga aaaacaaccg atttattgtt taaagggctc
      121 gcccgttttt gaatcgccct taggattatt cgaccccaat gaagatgggg tgattttaca
      181 ggaagattat gggatccccc tgcgctattt aaaaagtgtt atgtcgccct gggcggtgaa
      241 gcgtctgcat gaatttggcg gcgatatcag ccagtttaaa gtggtgaaag tttacccctc
      301 catactcgat caggtggcga tttctaaaac cgaaccgggc gatgagaata accaggatat
      361 ttccagcctt gtgggtaaag tggatattcg caagctggaa gaattcccgc aaaatgaccc
      421 tgatgcctat agtttctccg gcggtctgtg ccgtgccaac caaggcatta tggaatttgt
      481 ggagatgttt aaagcgccta ttaaggtttt gcacccctta ttaacggcga ctcaagaggg
      541 taattacaat tccactgaag gcttgggtgc aattcccttt gatggaacta ttttggcgca
      601 ctctaacgag tcggagtggc agaccttccg caacaacaaa aataatgaag cttttatcga
      661 ccgggtttat atcgtaaaag tgccttattg cctgcgcgtg tctgaagaaa tccatattta
      721 cgagaagctg ctgcaaaaca gctcgttgcg ccgcgcgccc tgcgcgccgg ataccttaaa
      781 aatgctcgcg cagtttgccg tgttatcccg cttgaaggtg ccggagaatt ctaatctttt
      841 ttccaaaatg cgcgtgtacg atggcgaaaa tctaaaagat acggacccta aggccaaatc
      901 tctgcaggaa tacaaagacg ccgccggagt ggatgagggt atgaatgggt tgtctacccg
      961 gtttgctttt aaaattctct ccaaagtatt taattttgac ccgacagaag ttgccgccaa
     1021 tccggtgcat ttgctgtatg tgttggagca gcaaattgaa caagagcaat tcccagaaga
     1081 gcgcgccggg cgttatttag ccttcttaaa agagtacctg gcgccacgat atgtggagtt
     1141 tatcggcaaa gaaattcaaa cagcttattt agagtcctat gcggaatacg gccagaatat
     1201 ttttgatcgc tacgttgtgt acgctgattt ctggatccaa gatcaggaat atcgcgaccc
     1261 tgaaaccggt gagattttaa accgtgccgc tattaatgaa gagttggaga aaattgagaa
     1321 gcctgcgggt attagtaatc caaaagactt ccgcaatgaa attgttaatt ttgtattgcg
     1381 ggcgcgagcg aacaacaatg gcaaaaaccc agtttggaat agctatgaaa aactgcgggt
     1441 ggtgatcgag aagaaaatgt tctcgaacac cgaagatttg ctgccagtga tttcattcaa
     1501 taccaaatcc tccaaagaag atcagaagaa acatcaggat tttgtggcgc gcatggtgga
     1561 acgcggttat accgagaaac aagtgcgtct gttgtctgag tggtatctac gggtgcgcaa
     1621 atcacaatag ggcattggtt gggcgacgtg taatgggcgt gagggattac gccgtgttaa
     1681 atgtttgtgc taggtaataa tcgctgtctg gagtgtgctg cccaggcaaa actaatataa
     1741 atagcaaaac taatataaat taggagagct gcgtgagcta cgtggtagat cgcaggttaa
     1801 atgccaaaaa caaaagtacc gttaatcgcc agcgcttttt ggatcgttat cgcgaccata
     1861 taaaaaaagc ggtgcagcag gctgttgata agcgttctat aaaagatatt gaatccggcg
     1921 agcagattag tattccgtcg cgggatatta gcgaacccac ttttcaccac ggccagggcg
     1981 gtgtgcgcaa tcgcgtgttg ccgggcaata aagaatttgt ctccggcgat aaaattcagc
     2041 gcccggaggg tggcggtggc ggctcgggca gcggtaaagg ggaggcctcg gattcgggtg
     2101 agggggagga tgaatttgtt tttcagatca cccaggaaga atttttaaac tatatgtttg
     2161 aagatttaga gctgcccaat ttagttaagc ggcagttggc aggaggtgaa gaatttaaat
     2221 accaccgggc gggtattgcc aatgaaggca acccgggtaa ggtaaatata gtgcgctccc
     2281 tgcgggcggc caatgcgcgg cgcatggcgc tgaccgggca aaaacgcaaa cagttgcgcg
     2341 cctatgaaga agagttggcg caattgactg aaggtgatga tcccttggtg gctgcgcgtc
     2401 gcaaagaatt agagcagtta atagttgggc tgaaagcgcg cattaagcgc gtgccctggc
     2461 tagatgattt tgacctgaaa tacaatctgc aggtaaaaca tccggtaccc agctccaagg
     2521 cagttatgtt ttgtgtaatg gatgtgtccg gctctatgga tcaggctact aaagatgtgg
     2581 ccaaacgctt ttttctattg ttgtatttat ttttacagcg cagttacggg cacaccgaag
     2641 tggtgtttat tcgccaccac accagtgcca aagaagtgga tgaacaggag tttttttaca
     2701 gccgcgaaac cggcggcact attgtttcca gtgccttaag gcttatggat gaaattattg
     2761 atgcgcgttt tccaacatct gaatggaata tttattgcgc tcaggcctca gatggcgata
     2821 actggaatga tgattcctcc gtctgctaca aaatgttggt ggagcacatc atgcagaagg
     2881 tgcagtattt ttcttatatc gaaattacgc cgcgggatca tcaggcgctc tggcgctctt
     2941 atgagcaggt agaggagagc tttcccgata gttttgctat gcgtcagatt cagtccttgg
     3001 ccgatattta tcctgtgttt cgcgatttgt ttcagaagaa ggcggcttag ctatggaaaa
     3061 acatcccatt tccaccggtt cggaatggag ttttgagtta attgaagcct acgataaggc
     3121 cattgccgaa attgcaaaag aatatcggct ggatacttat cccaaccaaa tagaaataat
     3181 tagctctgag cagatgatgg atgcctatgc atctgtgggt atgccgctgg ggtataacca
     3241 ctggtcctac ggcaagcagt ttttgaatgt ggagaagtcc tacaggcgcg gccagatggg
     3301 cttagcctat gaaattgtca tcaattccaa cccgtgtatt gcctatctta tggaagagaa
     3361 taccatgatg atgcaggcgc tggttattgc ccacgcgtgt tatggccata actcgttttt
     3421 taagggcaat tatttgtttc gcgcctggac ggatgcatcg tccattattg attatttggt
     3481 atttgcgaaa aactatattt cccagtgtga agaaaaacat ggcgtggcgg cggtggaggc
     3541 aattttagac tcctgccatg ccattatgaa ttacggtgtg gatcgttata aacgccctta
     3601 tcctatatcg gcccatatgg agcaggctcg gcaaaaagaa cgcgaggagt atttgcagcg
     3661 ccaagtaaac gatttgtggc gcactattcc tgcagatgaa caaaagaaaa atcagcagaa
     3721 acaatttcgt ttccctgaag agccccagga aaatttattg tacttttttg aaaaacacgc
     3781 gccgctgctt gaaccctggc agagggaagt ggtgcgtatc gtgcgcaaaa tagcccagta
     3841 tttttatccg cagcggcaaa cccaggtgat gaacgagggc tgggcgacgt tttggcacta
     3901 cacgcttatt aatactctgt ttgagcgtgg tttggtgacc gaaggtttta tgttggagtt
     3961 tatgcaatcc cacaccagcg tcatctatca gccgccgtat cacgctaaat attacaacgg
     4021 cattaatccc tacgcgcttg gctttgcgat gatgcaggat attcgccgta tttgtgagca
     4081 ccccaccgat gaagatcggc agtggttccc cgatatcgcc gggagcgatt gggtggctac
     4141 tttagatttt gctatgcgca attttaagga tgagagtttt attctgcagt ttttatcgcc
     4201 caaggttatg cgtgatttaa aactgtttac cattttggat gatgaccgca acagcacaat
     4261 tgatgttacg gcgattcaca atgattcagg ctataaagca gtccgcgaag cactgtcggc
     4321 gcaatataat ttgggcaatc gggagccgaa tattcaggtg tacagtgttg atgtgcgcgg
     4381 tgatcgctcg ctcaccttgc aacacattca gcataaccgc caaccgctgg atgcttcggc
     4441 ccaggaggtg ttaaaacata ttcaccgctt gtggggtttt gatgtgcggc tggattcaat
     4501 ctgcgatggt gcagttgcgc agagttttcg ctgttcgtcc aatcttgctg agccagtttg
     4561 attcgattat caggcatcaa atccattatt ttccaatagg catgctagtg atagcatgct
     4621 ttttttatgt tttttctgat tgagcgctat ggataaaatt ttattggtta caggcggtgc
     4681 tgttgggatc ggcgctgcaa ttgcgttgca ggccgctaag gcgggnnnnn nnnnncgcaa
     4741 tctgtgtaaa ttataactcc agtaaacagg ccgctgcgtc tttatgtgag cgaatttccg
     4801 cattgggggc aagagtgatt gcggttaaag ctgatgtcag cttagagcgg gatgtattgc
     4861 aactatttaa agaaatcgat agccagttgg gagtggttac ccatttggta aataacgcag
     4921 gtgttatcgc acctattaaa aaattcgagg caacgactct tgacgatttg cagcggactt
     4981 tttcggtaaa cgtatttggc agttttttat gcgctcggga ggcggtaaag cgaatgtgta
     5041 cgctcagcgg tggcaagggt ggcgtaattg tgaatatgcc ctcggtggct tcccgcattg
     5101 gctcacccaa tgaatttatt gaatatgctg cctcaaaagg tgcaattgat acatttacac
     5161 ttgggctttc taaagagttg gcgaaccgag gtatacgggt aaatgctgtg cggccgggtt
     5221 taattgatac tgaaattcat gcccatgctg gggatgccgc gcgccctgag cgattgaaaa
     5281 gcgccattcc tatgcaacgt attggaactg cctgcgaggt agcagaggct gtaatgtggt
     5341 tattgagcga cagcgccagt tatgtaacgg ggacaatact ggatgtggcg ggcgggcgtt
     5401 gatggggatt agtgggtaaa tgcctggaaa ttaataagcc ccttaacgac gagtctaggg
     5461 gctttttcac atagtcgata atttctataa cttaataaaa tgctcgcggt aataccgcag
     5521 ctccgccacc gaatcgcgaa tatcatccag cgccaaatgg ctgccttttt tggtgagtcc
     5581 cttgctaatt tccggtttcc agcgtttggc gagttctttc agggtggata cgtctaaatt
     5641 gcggtaggaa aaaaacgctt ccagttgcgg catgtaatgg gcgagaaagc ggcggtcttg
     5701 gcagatggaa ttgccgcaca tggggctttt gccggccggt acccattgct gtaaaaactc
     5761 cacggttgcc tgttctgctt gggcggtagt tgtcctgcta tcgagtaccc gctgcaccag
     5821 ccctgagccg ccgtgttgct gggtattcca ggcatccatg gcggcgaggg ttttagggtg
     5881 ttgattaatg gcgaacaccg ggccttcggc cagtatgttg aggtctttat ccgttaccag
     5941 ggtggcgatt tcgataatga catcagcctc cgggttgagg ccggtcattt ctaaatcgat
     6001 ccaaattaaa tggctatcgc agggcgatgg ttggggcatg gggttctcct ggtgggattt
     6061 ttctctacaa tggcgcaagt ttagcgtctc tccctccggg tggcgcctat tttttatttt
     6121 ggtactgcat gtctaaacgc cgcctcagtc tgcaacaaac ccgccgcatc gcccagcaaa
     6181 ataaccaggg ggtggagggc gatgtgaccg gctgcgaagg tgtggtgatt tcgcctttgc
     6241 gccagcaggc ggaaatagaa gacagcgcca ccggggagcg ctaccgctgc cacctgcggg
     6301 ccaacctgcc gccgcttacg gtgggggatc gcgtggtctg gcagcccggc tcgcccaccg
     6361 gggtggtggt ggcccttaag ccgcgccgca attgtattca aaggcccgat agcttcggca
     6421 atttaaagct ggtggcggcc aatgtggact gcgcgttaat tgtgctggcc ccggagcctg
     6481 tggcccacgc caatttaatc gaccgctacc tagttgcctt acacgccctg gacattcggc
     6541 ctgtattggt tttaaacaaa agcgatttat tagtccccga ccaccccatg ctggcccttg
     6601 ctgccagtta cgaggcccta ggctacacct gccttaaggt ttccgcccaa gctgccaaca
     6661 gcctggaacc tttgcagcgg gtgttggcag agggcacaag tattttggtg gggcagtcag
     6721 gggtggggaa atcctccctg ctgcaggcgc tcctgcctga tgagcagata aaaattggcg
     6781 agttgtccga gggtgtggcc aaagggcgcc acaccaccac ccatgcgcgc ctgtaccatt
     6841 ttgccggcgg cggtgcctgt atcgattcgc cgggtattcg cgagtttggc ttggggcatt
     6901 taagcccgga gcaggtggcc cagggctttg tggagtttga aggctttttg gggcagtgta
     6961 agtttcgcaa ttgtttacat cagcaggatc cgggttgcgc cctgctgcac gcgcgggagg
     7021 agggtgttat cagctctgaa cgctttgcca gttaccagca aattttggcc agccaaagtt
     7081 tgaccacgcc taagacatcc acaccatgac cctatccctc tccaccgccc gagcctcatt
     7141 ggctgcctgg gtgcttgaac ctgccgaggc cgagcgcctt ccgcctgaga ccctaattct
     7201 ggattgctcc agccaagccc aatacgaggc gggccactgg cctggtgccc tctggttgcc
     7261 gccgcagttt ttactctgcg gtaccgcccc ggtgccgggt aaacccccgc atatcagcga
     7321 gctcaatcgt ctgttcgatt gcttagggct ttaccctgag cggcctgtgt tggtgatgga
     7381 tgatgagggc ggcggctggg cggggcgcct gctgtggacc ttggatttaa tcggccacag
     7441 cccggcctat tacctcaacg gcggtgtgcg cgctttgtta agtgacggta tcgcccttga
     7501 acagacgatt caccaacccc aggcgatttc ccctattccc cgccaattac agcccgcctg
     7561 ccgcgtggag ttggatcagt tgctgggccg ctacagcagc acacaaatct gggacgcccg
     7621 cagcctgggt gagtatcaag gttggctgcg ggcggctacc tataccggcc atattccggg
     7681 cgcgcgccat ttggaatggt ccgagttata cgatccagcg cggggctatc gcatccgcga
     7741 ggatgctcta gggcggatta tcgaggcggg cttggatccg gccaggccca t
//
LOCUS       DAOQGS010000142         7772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4502_length_7772_cov_4.652974, whole genome shotgun sequence.
ACCESSION   DAOQGS010000142 DAOQGS010000000
VERSION     DAOQGS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4502_length_7772_cov_4.652974"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKZ52_07070"
     CDS             complement(<1..303)
                     /locus_tag="PKZ52_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNG59571.1"
                     /translation="MLVNATQAEEIRVALVDNQRLYDLDIESRQREQKKANIYKGKIT
                     RVEPSLEAAFVDYGAERHGFLPLKEISREYFARSERDGDGRTRIKDLIKEGTEVIVQ"
     gene            903..1856
                     /locus_tag="PKZ52_07075"
     CDS             903..1856
                     /locus_tag="PKZ52_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNG59572.1"
                     /translation="MAGGVTVQYFQVTDNEDGQRLDNYLLSRLKGAPKSLVYRVIRKG
                     EVRVNKGRVQPDTRLKTGDSVRVPPMRLSQEQAVVPVGEGLKQHLLASIIYDANGLLV
                     VNKPSGLAVHGGSGVSLGLIESLRQLPGNQGYLELVHRLDRETSGLVMVARKRSVLTY
                     LQNCLRERGTIQKTYLALVQGLWPKHRTQVNAPLLKSGEINQERFVKVAAQGKPSLTE
                     FNIVHTFAQCTLVEAKPITGRTHQIRVHAQYAGCPLIGDEKYGLAEVNSRFRSAGCRR
                     LFLHAARLDLVLPDGAALTLEAPLPEDLTAVLRALDAFGGQ"
     gene            1856..2509
                     /locus_tag="PKZ52_07080"
     CDS             1856..2509
                     /locus_tag="PKZ52_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNG59573.1"
                     /translation="MIAIFDWDGTLMDSTGKIITCMQQAASRVGLEVLGDRAIANIIG
                     LGLPEAVRTLYPYIDAAQAEQLRSSYSQIFIHEDTSPCNLFAGVEEGLQRLEAANIAL
                     AVATGKSRKGLNRMLAMLGWQERFAATRCADETASKPNPLMLQQILAELAAAPAGAVM
                     VGDTEYDLAMAAALNMPSLGLAYGAHEAERLHAHKPALVTDQFTLVVDWILARAAAT"
     gene            complement(2506..3090)
                     /locus_tag="PKZ52_07085"
     CDS             complement(2506..3090)
                     /locus_tag="PKZ52_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNG59574.1"
                     /translation="MPPIVLASSSSYRKALLSRLMLPFNTVQPDIDETPQACESPQAL
                     CERLSRAKAAAVSGQYSQAIVIGSDQVAALGTQLLGKPGNRARAFDQLTNCSGRPVDF
                     FTGLCVQLGGTQLFHLAHTRVHFRTLTPAEINRYIDVEMPLDCAGSFKAEGLGICLFD
                     RITSSDPTDLIGLPLIALTRMLREVGVNPLIATA"
     gene            3245..3751
                     /locus_tag="PKZ52_07090"
     CDS             3245..3751
                     /locus_tag="PKZ52_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNG59575.1"
                     /translation="MVPRLIEPRKFAQHGLQVAGEIPADSLQRLAGMALAVHSVAADL
                     KFSVAETKHRIVTGQFKGRVVMQCQRCLEPVELHLGGEVSLAMVWSEDDAKLLPSEYE
                     PWVVAQDQADLYEIIEEELLLALPLVPTHDYDCMDRSALAPKVVVEPGKHNNPFQVLA
                     RLKGSLTN"
     gene            3781..3963
                     /gene="rpmF"
                     /locus_tag="PKZ52_07095"
     CDS             3781..3963
                     /gene="rpmF"
                     /locus_tag="PKZ52_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNG59576.1"
                     /translation="MAVQQDKKSRSRRGMRRSHDALTAQTLSVDAATGEKHLRHHMTP
                     DGFFRGRKITASKDEE"
     gene            3966..4919
                     /locus_tag="PKZ52_07100"
     CDS             3966..4919
                     /locus_tag="PKZ52_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNG59577.1"
                     /translation="MAAAVRIAVDGMGGDFGPRLAFEACCIFLQQQPRAEIDLFVVES
                     FAAPFACPARLRLVMCPLAVLPADNPTQVLRHKQASSMGQALVQLRDGAAQACVSAGN
                     TGGLVMLAAHLLGIRAGVTRPVLCTAVPTAIGATWLLDLGGVLAPDADRLVEYAHLGA
                     QQARRVLKRPVTVALLNLGQEDEKGPLEVKAAAQRLKISSEFEYVGFVEPAGLFAGAA
                     DVVVCEGLVGNMVLKSAEAAAATVLSVVRREFSASPWRRLLALFCRGVFGRVRRQLHP
                     ARLNGALLLGVNGVVVKSHGGADQEGFLAALNVAYRAVGSD"
     gene            4934..5872
                     /gene="fabD"
                     /locus_tag="PKZ52_07105"
     CDS             4934..5872
                     /gene="fabD"
                     /locus_tag="PKZ52_07105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNG59578.1"
                     /translation="MSKSAFVFPGQGSQQVGMLAGLAAELPEVAQTFAESSEVLGYDL
                     WQLVSEGPQERLNLTEQAQPALLTASIALWRAWLARSSFRPSFLAGHSLGEWSALVAA
                     GVVSFTDAVRLVRLRGQYMQEAVPAGVGAMAAILGLDDAKVEQACRQASELGIVVPVN
                     FNSPGQVVIAGENAAVDAAMASANELGASKTMLLPVSAPFHTAMMRPAAERLAKDLAA
                     VAFAAPEIPVIHNLTARPEADPANLKALMIEQIYSPVRWVESIVYAVEQGVDTTVEVG
                     PGRVLSGLNKRIHKGLTLLATDDSARWQAALAHFQQ"
     gene            5923..6666
                     /gene="fabG"
                     /locus_tag="PKZ52_07110"
     CDS             5923..6666
                     /gene="fabG"
                     /locus_tag="PKZ52_07110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNG59579.1"
                     /translation="MSEQKVALVTGASRGIGAAIAEALGNSGLFVVGTATSEAGASAI
                     TERFAEAKINGVGKVLNVTDGESVEALVAEIAASHGPVAVLVNNAGITKDNLLMRMSD
                     EEWFDVINTNLSAVYRLSKACLRGMMKQRWGRIVNIASVVGAMGNAGQTNYSATKAGV
                     MGFTRSLAKEIGSRNITVNAVAPGFIETDMTRDLPEAQKAQLSASVALGRLGQPQEIA
                     NAVAFLVSDHASYITGETLHVNGGMYMSQ"
     gene            6857..7093
                     /gene="acpP"
                     /locus_tag="PKZ52_07115"
     CDS             6857..7093
                     /gene="acpP"
                     /locus_tag="PKZ52_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNG59580.1"
                     /translation="MSSIEERVKKIVAEQLGVKEEEVKNEASFVEDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQLAINYITTHLA"
     gene            7311..>7772
                     /locus_tag="PKZ52_07120"
     CDS             7311..>7772
                     /locus_tag="PKZ52_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNG59581.1"
                     /translation="MRNRVVITGMGMVSPLGNTVTDSWHALLAGTSGAQLIDSFDASA
                     FATRFSAPVKQLDLAPYLSEKDARKMDPFIRYGMVAGIQAMEDAGLEVTPANAHRIGC
                     IIGSGIGGLGSIEETHDTLLNKGPRRISPFFVPGAIINMVAGNLSIRYGMRG"
BASE COUNT         1622 a         1881 c         2388 g         1881 t
ORIGIN      
        1 ctgcacaata acttcggtgc cttccttgat gaggtcttta atgcgggtgc ggccgtcgcc
       61 atcgcgctca gagcgggcga aatattcgcg ggagatttct tttaagggta aaaagccgtg
      121 gcgctcagcg ccgtagtcaa caaaagcggc ttctagggag ggttcaacgc gggtgatttt
      181 tcctttatag atattggctt ttttctgttc gcgctgacgg ctttcgatat caaggtcgta
      241 caggcgctga ttgtccacca gggcaacgcg gatttcttcc gcttgggtgg cgtttactaa
      301 cattcttttg gtcatgatgt gctctcagaa agctaagccg gtaacaagcc gctagcgaga
      361 acagattaag aacggcaacc ggtggccgtg aaaacgaatg taccagtggg gcgggcctag
      421 cttctttgcg aagcaacctc aaggaccgac tttggttagg ccaggaatac taagatcagc
      481 gtgtctttag tgagctaagg cgccgcaaca gcggacgatt ataagacgat ggcgtcgagg
      541 acttaacttg gggtttgcta ttcccatcgc ctaaaaacgg ttgcagtggc ggtgggcctt
      601 aatattcttg gttgttttat tcttaatgtt ctgtgctaac gcactcatcg tgcggcgcaa
      661 ttaggcttca aaaattcgca tggaagccgg tttaaaactc gtttggtatc aacaacaggg
      721 tgggtcaaat tccgttgcac cattgcgcaa cgcttcacct acagttgaag acccgctgac
      781 tatagcacct ggttgaccgc tatacaaacc aatgcagttg gctatttcgc tagcctgcac
      841 tgcgtgctgg gtttgcacct cccaccgagc tacactgcgc gccgttaaac agtgtgaatt
      901 taatggcggg cggtgtgacg gttcagtatt ttcaggtaac tgataatgaa gatggccagc
      961 ggctggataa ttatttgttg tcccgtttaa agggggcgcc aaagtcgttg gtttatcgag
     1021 tgattcgcaa aggcgaggtg cgggttaata aggggcgggt gcagccggat acgcggctaa
     1081 aaaccggaga ttcggtgcgt gtgccgccta tgcggctgag ccaggagcag gccgtagtac
     1141 ctgtgggtga gggccttaag caacatttat tggcctctat tatttatgac gccaacggcc
     1201 tgctggtggt taacaaaccc agtggtttgg ccgtccacgg gggcagcggg gtgagcctgg
     1261 ggctgataga gtccctgcgg cagctgccgg gcaaccaagg ttatttggaa ttggtgcaca
     1321 ggctggatcg ggaaacatcc ggcctggtaa tggtggcacg caagcgcagt gtgctgacct
     1381 atttgcaaaa ttgtctgcgc gagcgcggca ccatccaaaa aacttatttg gctttggtgc
     1441 aggggctttg gccgaagcat cgcacccagg tgaacgcgcc gctgctcaaa agtggcgaga
     1501 tcaatcagga gcgctttgta aaggtggctg cccaaggcaa gccctctctt acggaattta
     1561 acattgtgca tacttttgcg cagtgcactt tggtggaggc aaagcccatt accgggcgca
     1621 cccatcaaat tcgtgtgcat gcgcaatatg ccggctgtcc gctgattggt gatgaaaaat
     1681 atggattggc agaggttaat agccggttcc gcagtgcggg ttgccgccgt ctgttcttgc
     1741 acgccgcccg cctggatctg gtattgcctg atggggccgc tttgacgttg gaggcgccgc
     1801 tgccagagga tttaactgca gttttgcgcg cccttgatgc attcgggggg cagtgatgat
     1861 cgcgatattt gattgggatg gcaccttgat ggattccact ggcaaaatca ttacctgtat
     1921 gcagcaggca gccagccggg tgggccttga ggtgctgggc gatagagcca tcgctaatat
     1981 tatcggccta ggtttgccgg aggccgtgcg cacactctat ccctatatcg atgctgcaca
     2041 ggccgagcag ctgcgcagta gctattcgca gatttttatc catgaagata caagcccctg
     2101 taatttgttc gccggggttg aggagggttt gcagcgtctg gaggcggcca atattgccct
     2161 ggcggtggcc acaggcaaaa gccgcaaggg gctcaatcgc atgttggcca tgctcggttg
     2221 gcaagagcgc tttgcggcga cccgctgtgc cgacgaaacg gcctctaagc caaacccctt
     2281 aatgttgcaa cagattttgg ccgaattagc tgccgcgccc gccggtgcgg tgatggtggg
     2341 cgacaccgaa tacgacctgg ccatggcggc cgctttgaat atgccgagcc tggggctggc
     2401 gtatggcgcc catgaggctg agcgtctgca tgcgcataaa ccggccctgg ttaccgacca
     2461 attcactctg gtggtggatt ggattttggc gcgagctgca gctacctaag ctgtggcgat
     2521 gagcgggtta acgcccactt ctctaagcat gcgagtaagc gctatgagtg gcaggccaat
     2581 taagtcggta gggtcgctgc tggtaatcct gtcgaataaa cagatgccca gcccctccgc
     2641 tttgaaactg cccgcgcagt ccaagggcat ctcaacatct atatagcggt taatttccgc
     2701 tggggtgagt gtgcgaaagt gcacgcgggt gtgagccagg tgaaataact gggtgccgcc
     2761 tagttgcaca caaaggcctg tgaaaaaatc gactgggcgg ccgctgcagt tggttagttg
     2821 gtcaaaagcg cgcgctcgat tacccggttt gccgagcagc tgggtcccta gggctgcgac
     2881 ctgatcggag cctatgacta tggcctgcga gtattggccg cttacggcag cggccttggc
     2941 gcggctcaag cgttcacaca atgcttgcgg gctttcgcag gcttgagggg tttcatctat
     3001 gtccggttgc acggtgttga acggcagcat taagcgggat aggagggctt tgcggtagct
     3061 ggagctggag gcgagcacaa taggtggcat aggagaatct gtcataagtt tgcaaaaaaa
     3121 gcgcaatcct aacaggctgg gagggttttt taggttaaat gcgcgggcag aggctaggtt
     3181 ttctttgaca cgggcagtgg atgccaatat gatgccgcgc ttatttttag ggtggttctg
     3241 ccccatggtt cctcgtctta tcgaaccgcg taagttcgcg cagcacggcc tgcaggtggc
     3301 gggggagatt cccgctgata gtctgcagcg tttggctgga atggctctgg ccgtccactc
     3361 agttgcggca gatcttaagt tttcggtggc tgagaccaaa caccgcatag tgactggcca
     3421 atttaagggc cgtgtggtta tgcagtgcca gcgctgtttg gagcctgttg aattgcatct
     3481 gggcggggag gtatctctgg ctatggtgtg gagcgaggat gacgccaagc tgttgccgtc
     3541 tgagtacgaa ccttgggtgg tggcgcagga tcaggcagac ctttatgaaa ttatcgagga
     3601 agagttgttg ctcgctctac ctctcgtgcc cacgcacgat tacgactgta tggatcgctc
     3661 agctctagcg ccgaaagtgg tggtggagcc gggcaaacac aataatccct ttcaggtgct
     3721 tgcacgcttg aaaggatcac tcactaacta attttcaacc agtgttgtag gagtacttta
     3781 atggccgttc aacaggataa aaaatcgcgt tcacgtcgtg gcatgcgccg ctctcacgat
     3841 gcgttgactg cacaaacctt gtcagtcgat gccgccaccg gtgagaaaca tctgcgtcac
     3901 cacatgactc cagatggttt tttccgcggt cgcaaaatta ctgcttctaa agacgaagag
     3961 taagcttggc cgcagcggtc aggattgccg tagatggcat gggcggggac tttggtcccc
     4021 gtcttgcgtt tgaggcgtgc tgtatttttt tgcagcagca accgcgcgct gaaattgatt
     4081 tatttgtcgt agagtccttt gccgcaccgt ttgcgtgccc cgcgcggttg cgtttggtga
     4141 tgtgcccgct ggctgttctt cccgccgata atcctactca agtgctgcgc cacaaacagg
     4201 cctccagtat ggggcaggcc ctggtgcagc tgcgcgacgg cgccgcccaa gcctgtgtca
     4261 gtgcagggaa taccgggggg ttggtgatgt tggcggccca tctgttggga atccgcgcag
     4321 gcgttacccg cccggtgtta tgcacagcgg tgcccaccgc cattggcgcc acgtggttgc
     4381 tggatttagg cggagtgctg gcgccggatg cggatcgact ggttgaatat gcccacttgg
     4441 gcgcccagca ggcccgcaga gtgttaaagc gtccggtcac ggtggcgcta cttaacctgg
     4501 ggcaagagga tgaaaaaggt ccactggaag taaaagcggc cgcgcagcgc ttaaaaatca
     4561 gcagtgaatt tgagtatgtg gggtttgtcg agcccgccgg tttgtttgct ggcgccgctg
     4621 atgtggtggt gtgtgaaggc ttggttggca atatggtttt aaaatccgcc gaagccgctg
     4681 cggccactgt gttgagtgtg gtgcggcggg agttttcggc atccccttgg cggcgcctgt
     4741 tggccttgtt ttgtcgcggt gtgtttggcc gtgtgcgcag gcagttgcat cctgcgcgcc
     4801 taaatggcgc actgctgttg ggggttaatg gcgtggtggt gaaaagccac ggaggggcgg
     4861 atcaagaagg ttttcttgcc gcgttgaatg tagcctatcg agccgtcggc tcagattaat
     4921 ttagggtgtt gttgtgtcta agagcgcatt tgtttttccg gggcagggtt cccagcaggt
     4981 gggaatgttg gcgggtttgg cggctgaact gcccgaagtg gcgcaaacct tcgccgaaag
     5041 ttcggaggta ttaggttatg acttgtggca attggtgagc gaaggccccc aggagcggct
     5101 aaaccttact gagcaggctc agcctgcatt gttaaccgca agcattgctc tttggcgcgc
     5161 ttggcttgcc cgcagcagtt tcaggccgag ttttttggct ggccacagtt tgggtgagtg
     5221 gtcggcgctg gttgcggccg gtgtggtgtc ttttaccgat gcggtgcgtt tggtgcgcct
     5281 gcgcggccaa tacatgcagg aggcagtgcc tgcgggtgtt ggcgccatgg cggcgatatt
     5341 gggcctggac gatgccaagg tcgaacaagc ctgtcgacag gccagcgaac tgggcattgt
     5401 ggtgccggtg aattttaatt cccccggcca ggttgttatc gccggtgaaa atgctgcggt
     5461 ggatgcggct atggcgagcg ccaatgagtt gggtgccagc aagacgatgt tgttgccggt
     5521 aagtgcgcca tttcataccg ctatgatgcg accggctgct gagcgactgg ctaaagactt
     5581 ggcggcggtt gcctttgccg ctcctgaaat ccccgtcatt cacaatctca ccgcccgtcc
     5641 tgaggcggac cctgccaatc tcaaagcctt gatgattgag cagatctact cgccggtgcg
     5701 ctgggttgag tccattgtgt atgcggtgga gcagggcgtt gatacgacgg ttgaggtagg
     5761 cccgggccgt gtcttgtccg gcttaaacaa gcgtattcac aagggtttaa cgcttttggc
     5821 aacggacgac tcggcccgct ggcaagcggc cctggcgcat tttcagcagt agttttagcc
     5881 aatccaattc cctttttatt gtttatttag gatgcaaacc acatgtcaga acagaaagta
     5941 gcactggtga ccggtgccag ccgaggtatt ggcgcggcta ttgctgaagc tttgggcaac
     6001 agcggtttat tcgtggtggg aacggccacc agcgaggccg gtgccagtgc catcacagag
     6061 cgttttgccg aagccaaaat aaacggcgtt ggcaaagtgc tgaacgtaac tgatggcgaa
     6121 tcggtggagg ctttggtggc ggaaattgcc gcctcgcacg gcccggtagc ggtactggta
     6181 aacaatgcgg gcattaccaa ggacaatttg ttgatgcgta tgagcgatga agagtggttt
     6241 gatgtgatta acactaacct cagcgctgtg taccgattaa gtaaagcctg cctgcgcggc
     6301 atgatgaaac agcgttgggg gcgaattgta aatattgctt cggtggtggg cgctatgggc
     6361 aatgccgggc aaaccaatta ctcggccacc aaggcgggcg taatgggatt tacccgttcg
     6421 ttggcaaaag aaattggctc gcgcaacatc acagttaatg cggttgcgcc cggctttatc
     6481 gaaactgata tgacccggga tctgcccgag gcgcaaaagg cgcaattaag cgcttctgtg
     6541 gctttggggc gtttggggca gccccaagag attgccaatg cagtggcgtt tttggtttcg
     6601 gaccatgcca gttacatcac cggtgagact ctgcatgtca atggcggcat gtatatgagc
     6661 caatagacga gcgcttcgct gtttatttac caatgtttag gggcgcgtcc acaagaaatt
     6721 tcccggcaag gcttacaaaa gggaaaaatc ccgttaaaat gcgcgcccaa gtcagccacc
     6781 ccagacgcga gcactatcga gcgacggcgg ctatctttgc ccaattccaa taaaggctac
     6841 gaggagttac ccacacatga gcagtatcga agaacgcgtc aaaaaaattg ttgctgaaca
     6901 actaggcgtt aaagaagagg aagtaaaaaa cgaggcttct ttcgtggaag atttaggcgc
     6961 ggattcttta gacaccgttg agttggtgat ggcgttagaa gaagaatttg aaactgaaat
     7021 tcccgatgaa gaagcagaga aaattaccac cgtgcaactg gcgattaact acatcaccac
     7081 gcaccttgct taatgtaagt tttttgcccc gcccgtgagt ataaaagccg tacctgtacg
     7141 gcttttgttt ttttggggcg cggcaattag tccggtggtg tctagggcct aaagtcaagg
     7201 cgcgcctaat ctgacggcca ccctcaaata ggatgcagac gctgtctgtt attgggttct
     7261 acggcgcggt tcggcctgcg tcggcacgac gtatttgaag tgaggccaac gtgcgaaatc
     7321 gagtggtaat taccggcatg gggatggtga gtcccttggg taatacagtt acagatagct
     7381 ggcatgccct gctggctggc accagcggtg cccaactgat tgattccttt gatgcttctg
     7441 ctttcgccac gcgctttagt gcgcctgtaa agcagctgga tctagcgcct tatctaagtg
     7501 aaaaagacgc tcgcaaaatg gatccgttta ttcgctacgg catggtggcc gggattcagg
     7561 cgatggagga tgccggttta gaagtcaccc cggctaatgc tcaccgcatc ggttgcatta
     7621 tcggctcggg tatcggcggt ttgggttcca ttgaagaaac ccatgacacc ctgctcaaca
     7681 aaggcccccg gcgcatttcg ccgttttttg tgcccggtgc cattatcaat atggtggcag
     7741 gcaacctctc cattcgctac ggcatgcgcg gc
//
LOCUS       DAOQGS010000143         7746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4526_length_7746_cov_4.542192, whole genome shotgun sequence.
ACCESSION   DAOQGS010000143 DAOQGS010000000
VERSION     DAOQGS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_7746_cov_4.542192"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKZ52_07125"
     CDS             complement(<1..345)
                     /locus_tag="PKZ52_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59582.1"
                     /translation="MTGLALIFIGYSLLAAISLALTQFRPAGYADAALPRVMGLVVLA
                     ALFVLQGAHFAWLYYNRPSVAGGLYQIALFAVAPAFYLFSQQVLNPAAPLAVTGRLAS
                     HGVPVLVAWALPN"
     gene            complement(550..975)
                     /locus_tag="PKZ52_07130"
     CDS             complement(550..975)
                     /locus_tag="PKZ52_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59583.1"
                     /translation="MITYFFWFTLLSVVITIGVITYKTRFWKTGAVVAAVVFFSSWAM
                     YHFKWEQSWVKNLGGTMTISVPKGLIHLGTTWKDENMWIENYDPKTNTCYFTEYAKGH
                     LLEGRVVIKHCNPALPSANPEPAEEPMSSEEAAKDTATQ"
     gene            complement(989..1858)
                     /locus_tag="PKZ52_07135"
     CDS             complement(989..1858)
                     /locus_tag="PKZ52_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNG59584.1"
                     /translation="MMRFNRFIGVALLMLSQACAAATSLWEVSKNNRVFYLGASISAL
                     KQKHYPLPAEFDDAYKKAAVLYVERDIAAVSAQDFSAQVMQRMMYSDGGNLKKQLRPE
                     VYQQLDAYSQSLGIPVFGLAMFKPAFSMVSLAAAQSAQGDYVYGVDTHYFYQAQESKK
                     NWRALETTNQQLELLDKINQEEPNQAVLFMLDELKNMPATLNKLVPAWRTGNLAAVDS
                     LGAGKMRKLTPQLYKALVVARHNNWLAQFNSMVQTPEEELVLLDIIHFTGPDSLLTLL
                     KKNGFNLTPYRKT"
     gene            complement(1874..2413)
                     /locus_tag="PKZ52_07140"
     CDS             complement(1874..2413)
                     /locus_tag="PKZ52_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59585.1"
                     /translation="MSNDVQRPFYLVSLSKLTILFFATQGAFALVWFFLHWQAQNTTQ
                     AKKVLSVPRAIFSIFYVGDLCNRLRKEQLKQGVEYFWSPMRLSFVFVAAYTSEFAIAL
                     GIHEQVLTSSWQYLALLLNLLEFYVVYQFQLVANRVMGDPFGKANSTITPINVLWIGF
                     GIIMWINLLRVWFGDAPSP"
     gene            complement(2498..2980)
                     /gene="greA"
                     /locus_tag="PKZ52_07145"
     CDS             complement(2498..2980)
                     /gene="greA"
                     /locus_tag="PKZ52_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNG59586.1"
                     /translation="MSFQKFPMTVQGEQKLREELDHLKKVERPRIVQAIAEAREHGDL
                     KENAEYHAAREQQSFCEGRIQEIEGKLSNAQVIDVASLPQNGKVVFGVTVSLLNVDTD
                     KHVVYQIVGEDEADIKVNKISVGSPIARALIGKEEGETVVVKTPGGDVEYEIEEVRYI
                     "
     gene            complement(2980..6198)
                     /gene="carB"
                     /locus_tag="PKZ52_07150"
     CDS             complement(2980..6198)
                     /gene="carB"
                     /locus_tag="PKZ52_07150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNG59587.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPAMADATYIEPITWQSVEQIIIKERPDAILPTMGGQTALNCALAL
                     HDNGVLAKYNVELIGAKQEAIHKAEDRNLFDKAMKAIGLECARARIVHSLAEALEVPK
                     EFGFPVIIRPSFTMGGSGGGIAYNWEEFEEICLRGLDLSPTHELLIDESLLGWKEYEM
                     EVVRDKNDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VETGGSNVQFAVNPETGRLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LQNDITGGATPASFEPSIDYVVTKIPRFTFEKFGDADARLTTQMKSVGEVMAIGRNFQ
                     ESLQKALRGLEVGSCGFESKIDWEDPANAVTIRHNLTTPGADRLWYVGDAFRSGMSLE
                     EVFAASKIDPWFLVQIEDLIKEEQTLLGAGLDALNAERLYSLKRKGFSDKRLGLLLGV
                     GEKAVRHHRQNLGIRPVYKRVDTCAAEFATDTAYMYSTYDEECEAAPSGRDKIMVLGG
                     GPNRIGQGIEFDYCCVHAALAAREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVHKEKPKGVIVQFGGQTPLKLARALEAEGVPIIGTSPESIDRAEDRERFQQMIE
                     KLGLKQPANAIVRSLEEAVSVADSVGYPLVVRPSYVLGGRAMEIVYSEKELRTYMRDA
                     VKASDDAPVLLDHFLNNAIEVDIDAVSDGKDVVIGGIMQHIEQCGVHSGDSACSLPPY
                     SLPEEVQNEMRAQVKKMALELEVVGLMNAQLAYQDGEVYIIEVNPRGSRTVPFVSKCI
                     GVSLAKVAARCQAGKTLAEQGFTQEIIPKYFSVKEAVFPFNKFPAVDPILGPEMKSTG
                     EVMGVGDTFAEAFAKAQTGAGSRIPTEGTVFISVRDFDKEHAVIVGKELADLGFKLVA
                     TRGTASRLEAAGLSVAVVNKMGEGRPNVVDMIKNREIDLVINTTEGKQAIKDSASIRR
                     SAENSRTYYTTTIAAATALCMALRFGQSQQVRRLQELHEGIV"
     gene            complement(6263..7402)
                     /gene="carA"
                     /locus_tag="PKZ52_07155"
     CDS             complement(6263..7402)
                     /gene="carA"
                     /locus_tag="PKZ52_07155"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNG59588.1"
                     /translation="MTTGLNKPAILVLEDGSVFRGKSIGAPGVSVGEVVFNTAMTGYQ
                     EILTDPSYAEQIVTLTYSHIGNVGANSEDEECARIWAKGLVIRDLSMLPSNFRSALSL
                     DAYLRKHNILGIADIDTRRLTRILRDKGAQNGCIMAGKVDESAALAKARSFAGLNGLD
                     LAKVVTTEKSFRWEAGSWQLGTGFSTLPEGERFKVVAYDFGAKRNILRMLVDRGCELT
                     VVPAKTPASEVLALNPDGVFLSNGPGDPAPCDYAIEAIKAILEANVPVFGICLGHQLL
                     ALASGAKTLKMKFGHHGGNHPVQDLKTGKVLITSQNHGFAVDEASLPANLVATHKSLF
                     DGSLQGIARTDKPAFSFQGHPEASPGPQDAAGLFDHFIDLMRARR"
BASE COUNT         1891 a         2118 c         1958 g         1779 t
ORIGIN      
        1 attgggcaga gcccaagcca ccagcaccgg caccccatgg ctggcaaggc gccctgtaac
       61 cgccagcggc gcggcggggt ttaatacctg ttggctaaac aggtaaaagg ccggcgcaac
      121 tgcaaacaag gctatctgat acaaaccgcc cgccaccgac ggccggttgt aatacaacca
      181 agcaaaatgc gccccttgca gtacaaacaa agccgccaac actaccagcc ccatcactcg
      241 ggggagggcg gcatcggcgt acccagcggg gcgaaattgt gtaagggcta ggcttatggc
      301 ggctaggagg gaataaccga taaatatcag ggcgagtccg gtcatcgaaa attcctaagg
      361 ttattgcagc agggaaacgt cacacacagt tcgatgtaaa aaaccgctcg gcgcaaacat
      421 tcaatgagca gcaaaatgga aacggcaagt agaatagaaa cggcaaatac agtggcagga
      481 ttaatctagc gccgagaaag agaaattgag cagccaaccc atgtagctgc ccaaatcaaa
      541 aaaacatgct tattgagtag ccgtgtcttt tgccgcctct tcactactca taggttcttc
      601 agcaggctca gggttagcgg agggcaaggc ggggttgcag tgtttaatta ccacacggcc
      661 ttctaataaa tggcctttgg cgtattcggt gaaataacag gtattggttt tgggatcgta
      721 gttttcaatc cacatgtttt catctttcca ggtggtgccc aaatgaatta agcctttggg
      781 cacgctgatc gtcatagtac cgcccaggtt tttcacccag ctttgctccc atttaaagtg
      841 gtacatagcc caggaggaga aaaatactac ggcggcaacc acggcgccgg ttttccaaaa
      901 acgggtttta taagtaatca caccgatggt gatgacaact gagagcaagg taaaccaaaa
      961 aaagtaggta atcataaaat acgcctaatt aagtcttgcg gtagggggta aggttaaaac
     1021 cgttcttttt taacagggtt agcaggctat caggcccggt aaaatgaata atatccagca
     1081 gtaccagttc ctcctcaggg gtttgcacca tgctattaaa ctgcgccagc caattgttgt
     1141 ggcgcgccac caccaaagct ttgtacagct gcggagtcag tttacgcatt ttgcctgcgc
     1201 caaggctatc caccgccgca agattgccag tgcgccaggc aggtacaagc ttatttaagg
     1261 tggcgggcat gtttttaagc tcgtccaaca taaataaaac cgcttggttg ggctcttcct
     1321 ggttaatctt gtccagcagc tctaattgtt ggttagtggt ttctaaagcc cgccagtttt
     1381 ttttgctctc ctgggcctgg taaaaataat gagtgtccac gccgtacaca taatcaccct
     1441 gggccgattg cgcagcggct aaactcacca tgctaaaagc gggtttaaac atggcaaggc
     1501 caaataccgg aatgcctaaa ctttggctat aggcgtccaa ctgttggtaa acctctgggc
     1561 gcagctgttt ttttaaattg ccgccgtcgc tgtacatcat gcgctgcatc acctgggcgc
     1621 taaaatcctg cgcacttact gctgcaatat cgcgctccac atacaatacg gcggcttttt
     1681 tataggcatc gtcaaactcg gcgggcagtg gataatgttt ttgcttcagc gcactgatgc
     1741 tcgcgcccaa ataaaacacc cggttatttt tactcacctc ccacaaagag gtagccgcag
     1801 cgcaggcctg gctcagcatc aaaagcgcca caccaataaa tctgttgaat cgcatcataa
     1861 atagcgttcg ctcttagggg ctgggggcat caccaaacca cacccgcaat aaattaatcc
     1921 acataatgat gccaaagcca atccacagca cattaatagg ggtaatggtg ctattggcct
     1981 tgccaaaagg gtcgcccatt acgcggttgg ccaccagctg aaactgatac accacgtaaa
     2041 attccagcag atttaacagc agtgccaggt actgccagga gcttgtcaaa acttgctcgt
     2101 gaatacccaa ggcaatcgca aattcagatg tataggcggc cacaaataca aaacttaacc
     2161 gcataggcga ccagaaatac tccaccccct gtttcagctg ctccttgcgc aggcggttgc
     2221 ataaatcccc cacataaaag atgctaaaaa tagccctcgg cactgagagt acttttttag
     2281 cttgcgtggt attttgcgcc tgccagtgca ggaaaaacca caccaaggca aaggcgcctt
     2341 gggtggcaaa aaacaaaatc gtcaatttac ttaaggacac caaataaaat ggacgctgca
     2401 catcattcga catagatttt tatacacaca aaaaaggcca ttcacatggc cttaaaataa
     2461 tcaggttcaa aaagcggtca actaaaaagc caggcaatta aatataacga acctcttcaa
     2521 tttcatattc cacatcaccc ccgggggttt tcaccaccac ggtttcgccc tcttctttgc
     2581 caatcagcgc gcgggcgatg ggtgaaccca cggagatttt attcacctta atatccgctt
     2641 cgtcttcacc cacaatctgg tataccacgt gtttatctgt gtccacatta agtagcgaca
     2701 cagtcacgcc aaacaccacc ttgccatttt gcggcaagct ggccacgtca atcacctggg
     2761 cgttagaaag tttgccttca atttcttgaa tgcgcccctc acaaaaactc tgctgctcac
     2821 gggcggcgtg gtattcggca ttttctttta aatcgccatg ctcgcgagct tcagcaatgg
     2881 cctgcacaat acgcggccgc tcaacctttt ttaaatggtc taattcttcg cgcagtttct
     2941 gttcaccctg cacggtcatt gggaattttt gaaatgacat tacacaatac cctcatgtaa
     3001 ttcttgtaaa cggcgcacct gttgggattg cccaaagcgc agcgccatac ataatgcagt
     3061 ggcagcagca attgtcgtgg tgtaataggt gcgggaattc tccgcactgc ggcgaatact
     3121 ggcagagtct ttaatcgcct gtttgccttc ggtggtgtta attaccaagt caatttcgcg
     3181 gtttttaatc atatccacca cgtttggcct gccctcgccc attttattca ccaccgcaac
     3241 acttaagccc gctgcttcta agcggctggc agtgccacgg gtggccacta gtttaaagcc
     3301 taaatccgcc agctctttac caacgatgac cgcgtgttct ttatcaaaat cccgcacgct
     3361 gataaacacc gtgccctcgg tggggatgcg cgagccggca ccggtttgcg ctttggcaaa
     3421 ggcttcggca aaggtatcgc ccacccccat cacctccccg gtggatttca tttccggccc
     3481 caagattggg tctacggcgg ggaatttatt aaacgggaat accgcctctt ttaccgagaa
     3541 atacttaggg ataatttctt gggtaaagcc ctgctcggca agggttttac cggcctggca
     3601 gcgggcggcg actttggcta aagacacgcc gatgcatttg ctcacaaacg gtacggtgcg
     3661 cgaaccgcgg gggttcacct caatgatata cacctcgccg tcttggtagg ccagctgagc
     3721 gttcatcaaa cccactacct caagctccag ggccattttt ttcacctggg cgcgcatctc
     3781 attttgcacc tcttccggca gggaataagg cggcagagaa caggcggagt caccggagtg
     3841 cacgccgcac tgctcgatat gctgcatgat gccgccgatg accacatctt taccgtccga
     3901 taccgcatca atatccacct cgatggcgtt attgaggaag tgatcgagca ataccggcgc
     3961 atcgtcggag gcttttaccg catcgcgcat ataagtgcgc agctctttct cggagtacac
     4021 aatctccata gcgcggccgc ccaatacata ggaggggcgc accaccagcg ggtaaccgac
     4081 ggaatcggcc acactgacgg cctcttccaa agagcgcaca atggcattgg caggctgttt
     4141 aaggccaagc ttttcgatca tctgctggaa gcgctcgcga tcctccgccc ggtcaatgga
     4201 ctcagggctg gtgccgataa taggcacgcc ttccgcctct agggcgcggg cgagtttcag
     4261 cggcgtctgg ccgccgaatt gcacaatcac ccctttgggt ttttctttgt gcacaatctc
     4321 caacacatct tctaaggtaa ccggctcaaa atagaggcgg tcggaggtgt cgtaatcggt
     4381 ggagacggtt tcagggttac agttcaccat gatggtttca aagccatctt cgcgggcggc
     4441 gagagctgcg tgcacacagc agtagtcaaa ctcaatgccc tggccgatgc ggttggggcc
     4501 gccgccgagc accataattt tatcccgccc agagggcgcc gcttcacact cctcatcgta
     4561 ggtggagtac atataagcgg tgtcggtggc aaattccgcc gcgcaggtat ccacgcgctt
     4621 atacacgggg cgaataccca gattctgccg gtggtggcgc acagcttttt cacccacacc
     4681 cagcagtaac cccaggcgct tatcggaaaa ccctttgcgc tttaggctgt acaaccgctc
     4741 ggcattgagt gcatccaaac ccgcacccag cagggtttgt tcttctttaa tcaggtcttc
     4801 tatctgcact aaaaaccaag ggtcgatttt tgaggcggca aatacttcct ctaggctcat
     4861 gccggagcgg aaggcatccc ccacgtacca caggcggtcg gcgccggggg tggtgaggtt
     4921 atggcggatg gtgacggcat tggctgggtc ttcccagtcg attttgctct caaaaccaca
     4981 ggagcctacc tccaaaccgc gcagggcttt ttgcagggat tcctggaagt tgcggccgat
     5041 cgccatcacc tcacccacgg atttcatctg ggtggttaaa cgggcatcgg catcgccaaa
     5101 tttttcgaag gtaaaacgcg ggattttagt gaccacataa tcaatggacg gctcgaagga
     5161 ggccggtgtg gcgccgccag taatatcatt ctgcagctca tccaatgtgt aacccaccgc
     5221 cagcttggcc gccactttgg caatggggaa cccggtagct ttagaggcca gggccgacga
     5281 gcgcgacacc cgcgggttca tttcgattac aactaaacgg cccgtctccg ggtttaccgc
     5341 aaactgcacg ttggagccgc cggtttccac acctatctcg cgcagcaccg caatggaggc
     5401 gttgcgcata atttggtact ccttgtccgt cagggtttgc gcaggcgcta cggtaataga
     5461 gtcaccggtg tgcacaccca tagggtcaaa gttttcgatg gagcagacga taatgcagtt
     5521 gtcgttttta tcccgcacca cttccatctc gtactctttc cagccgagca gggattcgtc
     5581 aattaacagt tcatgggtgg gggataaatc caaaccgcgc aggcagattt cttcaaactc
     5641 ttcccagtta taggcgatac cgccgccgga gccgcccatg gtaaaggagg ggcggataat
     5701 taccggaaag ccaaattctt tcggcacttc caaggcttcc gccaggctgt gcactatgcg
     5761 ggcgcgggcg cactcaaggc cgatggcctt catggcttta tcaaacaagt tgcggtcttc
     5821 cgccttgtga atggcctctt gtttggcgcc gatcagctcc acattgtatt tggccaatac
     5881 gccgttatcg tgcagagcca gggcgcagtt aagggccgtt tggccaccca tggtgggcaa
     5941 tatggcgtcc gggcgctctt tgataataat ttgctccacc gattgccagg tgatgggctc
     6001 gatgtaggtg gcatccgcca tagcggggtc agtcatgatg gtggcggggt tggagttcac
     6061 caggatgacg cggtagccct cttctcgcag ggccttacag gcctgggcac ctgagtagtc
     6121 aaactcacag gcctggccaa tcacaatggg gcctgcgcct atgatcaaga tgctttttaa
     6181 atcggtacgt tttggcatgt cagtcgtcta gctaattagc ggcaaaagtc ggggcaaaag
     6241 tcagtgcaaa taagggcgcg cattagcggc gcgcgcgcat taaatcaata aagtgatcaa
     6301 acaaaccggc cgcatcctgg gggcctgggc ttgcctcagg gtggccttgg aaactaaacg
     6361 caggcttatc cgtgcgcgca atcccctgca aggagccatc aaacagagat ttatgagtgg
     6421 ctaccaggtt ggcaggtagg ctcgcttcat ccaccgcaaa gccgtggttt tggctggtaa
     6481 ttaacacctt gccggttttt aaatcctgca ccgggtggtt gccgccgtgg tggccgaatt
     6541 tcattttgag ggttttagcg ccggaggcga gggccaataa ctggtgcccc aaacaaatgc
     6601 caaacaccgg cacattggcc tctaagatag ctttgattgc ctcaatggcg taatcgcacg
     6661 gcgccgggtc cccagggcca ttggagagaa acacaccgtc ggggttgagc gccaatactt
     6721 cactggccgg ggttttggca ggcacaacag taagctcaca accgcgatcc accaacatac
     6781 gcaaaatatt gcgcttggcg ccaaagtcat aggccactac cttaaagcgc tccccctcag
     6841 gcagggtgct aaaacctgta cccaactgcc aggagcccgc ctcccagcgg aagctttttt
     6901 cagtggtgac cactttagcc agatccaaac cattcaaacc ggcaaagctg cgagccttag
     6961 caagggcggc ggactcatcc accttgcccg ccatgatgca gccattttgc gcgcccttat
     7021 cccgcaggat gcgagttaaa cggcgggtgt ctatatcggc aatacccagg atattgtgtt
     7081 tgcgtaggta ggcatccagg cttaaggcgc tgcgaaagtt gctgggcagc atggataaat
     7141 cgcggattac caggcccttg gcccaaatgc gggcgcattc ttcatcttca ctattagcgc
     7201 ccacattacc tatatgggaa taggtgagcg taacaatttg ctcggcgtaa gaaggatcgg
     7261 tgaggatttc ctgatatccg gtcatagcgg tattaaacac cacctcccct acagataccc
     7321 caggtgcccc gatggatttc ccccggaaca cactgccatc ttccaacacc aaaattgcgg
     7381 gtttattgag cccagttgtc aaaatcttgt cctccagcca agcgcaaggc ttaaaaaagc
     7441 ccacgcgatt acgggtattg ctttacgagt actgccttta caggcagttc cactaaatct
     7501 caggtgcgca caaaaccgag gccgcagtaa aactgaagcg ccggtgcgat gaaatgtcgg
     7561 gttgtaaaaa agcgaggtgc aactgcgcgc tccacctcgc tctaatgaat atctcggcgc
     7621 ctaatacagc taatgcctta ctaaggctgg caccaagcgg gcactcggcc cgaactgggg
     7681 gcgcatttta tggtacgggg ggtattttgt ccacttaggt ttcgcgcctt gtttgccaag
     7741 cataaa
//
LOCUS       DAOQGS010000144         7742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4530_length_7742_cov_4.037856, whole genome shotgun sequence.
ACCESSION   DAOQGS010000144 DAOQGS010000000
VERSION     DAOQGS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4530_length_7742_cov_4.037856"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="pssA"
                     /locus_tag="PKZ52_07160"
     CDS             complement(<1..825)
                     /gene="pssA"
                     /locus_tag="PKZ52_07160"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNG59589.1"
                     /translation="MTDPNPVEPSQEADNTNQPPASLQIPIDEHFENVSQNGKTVRRK
                     GVYLLPNLITTAALFSGFYAILAGVNGRFEQAAIAIFVAQLLDGFDGRVARLTNTTSA
                     FGVQYDSLSDMVSFGVAPAVVMFAWGLEPLGKLGWAAAFAYAACAALRLARFNTQVDT
                     VDKRYFVGLASPAAAALMASTVWLFNEDQPSAQFSLLLAVMTVMAALLMVSNIRYNSF
                     KGLGLKGRVPFIAMLAVVLGFVVITIKPALALLSIALAYAASGPVFWVKNRSNSTEN"
     gene            complement(935..1951)
                     /gene="ilvC"
                     /locus_tag="PKZ52_07165"
     CDS             complement(935..1951)
                     /gene="ilvC"
                     /locus_tag="PKZ52_07165"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNG59590.1"
                     /translation="MHVYYDKDCDLSIIQNKKVAIIGYGSQGHAHANNLKDSGVSVVV
                     GLRPGSPTAQKAEAAGLAVKSVPEAVASADVVMILTPDEFQKQLYRDEIEPNLKKGAT
                     LAFAHGFAIHYNQVVPRADLDVIMIAPKAPGHTVRSEFVKGGGIPDLIAIYQDATGNA
                     KKLALSYAAGVGGGRTGIIETTFKDETETDLFGEQAVLCGGTVELVKMGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPKVINEESRKAMRA
                     ALKDIQQGEYAKKFILEGAANYPSMTAWRRNNAAHPIEQVGAKLRGMMPWIQANKIVD
                     KSKN"
     gene            2054..2914
                     /gene="ilvY"
                     /locus_tag="PKZ52_07170"
     CDS             2054..2914
                     /gene="ilvY"
                     /locus_tag="PKZ52_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HNG59591.1"
                     /translation="MDTDKLKALLVLAETLNFSRAADQLHMSPSTLSRVITQIEQEFK
                     VALFVRNNRSVQLTDAGKRCYEYAKSTLAEFSSLQQELMQASDVLKGDISIYCSVTAS
                     YSFLHEILAQFRQDHPHIRFLLHTGDHAQALERISQGREDVAIAARPDLLRQGIVFKA
                     ITQSPLVFIEPTHTAERTPEDINWLELPLILPEQGVGRARIDRWFAHLGIRPLIQAQV
                     GGHEAIVSMVSLGFGVGLVPKVVVDNSPLAQQVRLFCIQPDIKPYEVGVCVLEKRLKS
                     PLVRAFWSKL"
     gene            3268..3672
                     /locus_tag="PKZ52_07175"
     CDS             3268..3672
                     /locus_tag="PKZ52_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59592.1"
                     /translation="MVCYANTCRADRSIFCGAADQLKKIQFIPIRQMDNDMVLESVAN
                     TLDSSMSSEVRLLNHYWLNSTEDNTKVNSAAANRVVAITLATYYDLAMQSLIPGEPDK
                     HAVEHTALVGILGDYNLRVSKHSAQIGWNFRF"
     gene            3848..5395
                     /locus_tag="PKZ52_07180"
     CDS             3848..5395
                     /locus_tag="PKZ52_07180"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNG59593.1"
                     /translation="MSKQHLVIFDTTLRDGEQSPGASMTKDEKVRIAKMLEKLRVDVI
                     EAGFAIASPGDFAAVQAVARAVKDSRVCSLARAVLGDIDRAGEALKGANASRIHTFIA
                     TSPIHMQYKLQMQPDQVLEQAVRAIKHARQYTDDVEFSLEDGSRSEFEFMCRIVEAAI
                     NAGASTINVPDTVGYGEPAAYGAMFKRLIEAVPNADKAIFSTHCHNDLGLAVANSLSA
                     VMNGVRQVECTINGLGERAGNAALEEIVMAVRTRKDVFPVETRINAEHIVATSRLVSS
                     ITGFAVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWHTNKLVLGKLSG
                     RAAVKARYEELGISFDTQEELDEVFKRFKALADKKTEIFDEDLHSLVSHGSEHTVPEH
                     YELVDLEVCSKTGTTPKAQISLKVHGELKQAVAEGSGPVDASYKAIEKIVDSQSHLQL
                     YSVNAITSGTDSQGEVTVRLERKGLIVNGIGSDTDIVAASAKAYLHALNLLEGGRQRS
                     HPQHDQV"
     gene            5399..6169
                     /locus_tag="PKZ52_07185"
     CDS             5399..6169
                     /locus_tag="PKZ52_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59594.1"
                     /translation="MTEQRRMEYLDALGIEQFVPRKQLPGAKPSLALIRSEGPAASMP
                     SPQAQPLPAKPGPQEPAPPEPVKAQALQPVAQIVSQVAERLSAAQMGDTPKHSSRQLT
                     PSSLVPPQKVLRFALTVWHVPGWLWIDSRQSQQALPTDALLQNMLWALGLREPVKPEI
                     IKWPPVESPLQVQDWQQAREMLRSFLTPRLGPGERLILMGEAAYNACTQGPFDYAERL
                     AAGDMQHPSNPCVLLPSLAQILSEPQLKARIWRQLQQV"
     gene            6172..6660
                     /gene="rimI"
                     /locus_tag="PKZ52_07190"
     CDS             6172..6660
                     /gene="rimI"
                     /locus_tag="PKZ52_07190"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNG59595.1"
                     /translation="MAPFTACSLPSPLGPLHFCALGLEHLNEIDQLEQASSPHPWSRA
                     NIASSLGSSHRGLALRWEGKLFAYGFVSIASVEAELLIITVAKKRQGQGFGRLLLATI
                     IEQLKGRVSEMFLEVRPSNTAALALYESLGFNQVGVRPRYYPGPRGGEDAWVMGLCLE
                     AE"
     gene            complement(6680..7102)
                     /locus_tag="PKZ52_07195"
     CDS             complement(6680..7102)
                     /locus_tag="PKZ52_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNG59596.1"
                     /translation="MWLSKHISLKARPRGFHLITQEITDALPEITRLRVGLLHVFIQH
                     TSAALCINENADPSVRRDFLTVFNRLIPESSSAYEHNDEGLDDLPAHIKASLLGSSIS
                     LPVANGRLQLGTWQGVYLCEHRDHGGARRLVLTLQGED"
     gene            complement(7105..>7742)
                     /locus_tag="PKZ52_07200"
     CDS             complement(7105..>7742)
                     /locus_tag="PKZ52_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59597.1"
                     /translation="KYLLHSSRYLHKKVHPLLLPIAAKCVDTVLNLTRKWLGRGKKKP
                     WQIAPVLNFTLHAQQLDALMDSYKDTLWVLRQRSADFLQWRFLTNPRRQYNALGLFHE
                     GLLAGYAVYHIKGKSHLHIDDLFTAANNLKGLQALVVALLEIASLASVDLVCFTAHNE
                     LWQTLMIPMGFTFREDAHLFGVAVKDSELQAHFIDPTRWWVTSCDTHSEGF"
BASE COUNT         1869 a         2058 c         2078 g         1737 t
ORIGIN      
        1 gttttccgtg ctatttgatc tatttttcac ccaaaaaacc gggcctgaag cggcataggc
       61 taaagcaata ctcagaagtg cgagagcagg cttaatggtg ataacaacaa aacctaaaac
      121 taccgccaac atcgcaatga aaggcactcg ccctttaagg cccaagccct taaaactgtt
      181 gtaacggata ttagacacca tcaagagagc ggccataacg gtcataacag ccaggagcaa
      241 ggagaattgg gcgctgggtt gatcctcatt gaacaaccac acggtagagg ccatcagcgc
      301 cgctgcggca gggctagcta ggccaacaaa atagcgcttg tccaccgtat caacttgcgt
      361 attaaaacgc gccaagcgca gtgccgcaca ggcggcgtag gcaaatgctg ccgcccagcc
      421 aagctttcca agcggttcta gcccccatgc gaacattaca acggctggcg ccacaccaaa
      481 ggacaccatg tccgagaggc tgtcgtattg cacaccaaat gcgctggtgg tattggtcaa
      541 tcgcgccaca cgaccgtcaa aaccatccaa taattgcgcg acaaaaatcg cgatggccgc
      601 ctgctcaaag cgcccattca cacccgccaa aattgcatag aagcccgaga aaagcgctgc
      661 tgttgtgatt agatttggta aaagatacac gcctttgcgg cgcacggttt tgccattttg
      721 ggagacattt tcaaagtgct cgtcgatggg tatctgcaaa gaggctggag gctggtttgt
      781 attgtcagcc tcttgagatg gctcgactgg attgggatct gtcataggaa ttttccgcat
      841 gtcaccaatg aaaaacgcgg ccacagccgc gtttttggaa tctatcgacc cgcaccgacc
      901 tgaggctcgg cggcgggccg acgggaagtt actcctagtt tttgctttta tcaacaatct
      961 tgttggcttg aatccagggc atcatgccgc gcaatttggc acccacttgt tcaatgggat
     1021 gagctgcatt gttgcgacgc catgcggtca tggatgggta gttggcggcg ccttcgagga
     1081 taaatttctt ggcgtattcg ccctgttgaa tgtctttaag ggctgcacgc atggccttgc
     1141 gggattcttc gttgatcact ttgggcccgg tcacgtactc accgtactcc gcattattgg
     1201 agatggagta gttcatgttg gcgataccgc cttcgtacat caaatcaacg atcaatttaa
     1261 gctcgtgcag acattcaaag taggccatct caggggcgta acccgcttca acgagggttt
     1321 caaagcccat ttttactaac tcaaccgtgc cgccgcacaa tacggcctgt tcaccgaaca
     1381 ggtcggtttc ggtttcgtct ttaaaggttg tttcgatgat gccggtgcgt ccaccgccca
     1441 caccggctgc ataggagagc gcgagctttt tggcgttgcc ggtggcgtct tgatagatag
     1501 caatcaggtc agggatgccg ccgcctttaa cgaattcgga gcgcacggtg tggccggggg
     1561 ccttaggtgc aatcatgata acgtcgaggt cggcgcgcgg taccacttgg ttgtagtgaa
     1621 tggcaaagcc gtgagcaaag gccagagtgg cgcctttttt caggttaggc tcgatttcat
     1681 cgcggtagag ttgcttttga aattcatcgg gagtgaggat catcactaca tcggcgctgg
     1741 caacggcttc aggtacagat ttaaccgcaa ggcccgcagc ctccgctttt tgcgcagtgg
     1801 gcgaacccgg gcgcaaaccc acaaccacac ttacaccgga atctttcagg ttgttggcat
     1861 gggcgtggcc ttgggagccg tagccgatga tggctacttt cttgttttgg ataatcgata
     1921 aatcgcaatc tttgtcgtaa taaacgtgca tgagcgtgtc ctctggagtg atttggtggc
     1981 agtgtagaac ctggctagtg ttccgtaaaa tgatatattc gcaatcattt attgcaattt
     2041 ataaaaccac actatggata cagataaact caaagcgctg ctggtactgg cggaaaccct
     2101 caactttagc cgagcggccg accaactcca tatgagccca tcgaccttaa gccgggtcat
     2161 cacgcaaata gagcaggagt tcaaggtggc tttgtttgtg cgcaacaacc gcagcgtaca
     2221 gcttacggac gcaggtaagc gctgttatga atatgccaaa agtactttgg ccgagttttc
     2281 cagcttgcag caggagttaa tgcaagccag cgatgtgtta aaaggcgata tcagtattta
     2341 ttgttcagtc accgcgagct acagcttttt acatgaaatc ctcgcgcaat ttcgccaaga
     2401 ccacccgcac atccgtttct tgttgcatac cggcgatcac gcccaggcgt tagagcggat
     2461 ttcccaaggc cgggaagatg tggccatcgc cgcgcggccg gacctgttac ggcagggcat
     2521 agtatttaaa gccattaccc agtcgccgct ggtttttatt gaacccaccc ataccgccga
     2581 gcgcacacct gaggacataa attggctcga attacccctg atattgcccg agcagggtgt
     2641 cgggcgggcg cgcattgacc gctggtttgc ccatttaggt atccggccct taatccaggc
     2701 ccaggtgggt ggccatgagg cgattgtgag catggtgagt ttgggatttg gcgtaggctt
     2761 agtgcctaaa gtggtggtag acaatagccc tttagcccag caggtgcgtt tgttctgcat
     2821 tcagccggat attaaaccct atgaggtggg tgtatgcgtg ttggaaaagc gcttaaaaag
     2881 ccctttggtg cgggcctttt ggagcaaact gtaaaagctg caatagaaaa ctattcaagg
     2941 ttctttaagc aacacgccat tcgatgcatg atttttctct atcagtattt ggccgaatat
     3001 agttatgaag aatggataaa aatattattc ccatcataaa gcccatcatt ttgcgctaag
     3061 tggttatagg ttttatatca atccagaaaa attattctaa ctctccacgc tacttttatc
     3121 gcctgagctg aaccattctt aagaagcagg gaacgccgcc tgttttagcg cggcacccta
     3181 ctggtttgaa cacgaaaaaa gacggtgaat aatgtggtgt gcaagtggta tcaactgaaa
     3241 ataggctcag taatttgcgg tgcaattttg gtgtgttatg caaatacatg ccgcgctgat
     3301 cgatctattt tttgtggtgc tgccgatcaa ttaaagaaaa ttcaatttat tcccatccgg
     3361 caaatggata acgatatggt gctggagtcc gttgccaata ccctcgatag cagtatgagc
     3421 agcgaggtgc gcctgctcaa tcattactgg ctcaacagca ctgaagacaa caccaaggta
     3481 aatagcgccg ccgccaaccg cgtggtagcg attaccctcg ccacttatta cgacttggcc
     3541 atgcaaagcc tcatccccgg tgagccggat aagcacgcgg tggagcatac agcgctggtg
     3601 ggcattttgg gggattacaa tctgcgcgtc tccaagcatt cggcccaaat aggctggaat
     3661 ttccgctttt aaatactgcg gggcttaaag cttccgcccc aaataacaac acgcttcaca
     3721 ccaccggcat ctgccgctaa agcttgcgca acccatccgt cttcacaatc actgcagcct
     3781 gtgctgtccg gccccggcca attttggtta gaatggcggc cttttggatc ggctgcgccc
     3841 aacttttatg agcaaacaac accttgtaat tttcgatacc actttgcgcg atggcgagca
     3901 gagccctggc gcttccatga ctaaagacga aaaagttcgc atcgccaaaa tgctggaaaa
     3961 actgcgggtt gatgtgattg aagccggttt tgccatcgca agccccggtg attttgcggc
     4021 ggtacaggcg gtggctcggg cggtgaaaga ctcccgcgtc tgtagcctgg cccgcgccgt
     4081 gctcggcgat atcgaccgcg caggcgaagc cctcaaaggg gccaacgcct cgcgtattca
     4141 cacctttatt gccacttcac ccattcacat gcaatacaag ctgcaaatgc agccggatca
     4201 ggtattggag caagccgtgc gggccattaa acatgcccgc cagtacaccg acgacgtgga
     4261 attttctttg gaagacggca gtcgctcgga atttgagttt atgtgccgca ttgttgaggc
     4321 cgctattaat gccggcgcca gcaccataaa cgtgccggat acagtgggtt atggggagcc
     4381 tgccgcctat ggcgccatgt tcaaacggct tattgaagcg gtgcccaatg cagataaggc
     4441 cattttctcc acccactgcc acaacgattt gggcctggcg gtggccaact ctttgagcgc
     4501 cgtgatgaat ggtgtacgcc aggtggaatg caccatcaat ggcctgggtg agcgggcggg
     4561 caatgcggcc ctggaagaaa ttgttatggc cgtgcgcacc cgcaaggatg tgtttccggt
     4621 ggaaacgcgc atcaacgccg aacacatagt ggctacctcc cgcctggtgt ccagcattac
     4681 cgggtttgcc gtgcagccaa ataaggccat agtgggggcg aatgcctttg cccatgaatc
     4741 gggcattcac caggatggcg tactgaaaca ccgggaaacc tatgaaatta tgcgcgcgga
     4801 ggatgtgggc tggcatacca ataaactggt gctcggcaag ctctccggcc gagcggcggt
     4861 taaagcccgt tatgaagagc tgggcatcag ctttgataca caagaagaat tggatgaggt
     4921 gttcaaacgc tttaaggctc tggccgataa aaagacggaa attttcgatg aagacctgca
     4981 cagcttagta agccacggca gcgaacacac ggtgccagag cactacgagc tggtggactt
     5041 ggaggtgtgc agcaaaaccg gcactacgcc taaggcccag atcagcctga aggtacatgg
     5101 cgaattaaaa caggcggtgg cggagggctc cggccccgta gatgccagtt acaaagccat
     5161 cgagaaaatc gtcgatagcc aaagccattt gcaactctat tcagtcaacg ccattacctc
     5221 cggcaccgac agccaagggg aagtgaccgt gcggctggag cgcaaaggct tgatcgtcaa
     5281 tggcatcggc tcggatacag acatagttgc ggcctcagcc aaggcgtatt tacatgccct
     5341 caacctgctg gaaggcggcc gccagcgctc ccatccacaa cacgaccagg tataagccgt
     5401 gactgaacag cgccgcatgg aatacctcga tgctctgggc attgagcaat ttgtgccgcg
     5461 caaacaactg ccgggtgcta agccttcctt ggcactgatt cgcagcgaag gccccgcggc
     5521 atctatgccg tcgccacaag cgcagccgct gccagcaaag ccgggccccc aggaacccgc
     5581 gcccccagaa cccgtgaagg cccaggcctt gcaaccggtt gcccagatcg taagccaggt
     5641 ggctgagcgg ttgtcggccg cgcagatggg tgacaccccc aaacacagct cccgccaact
     5701 aacacccagc agcctcgttc ccccccaaaa ggttttgcgc ttcgccctca cggtgtggca
     5761 tgtgcccggc tggctgtgga ttgactcgcg gcaaagccag caggcactgc ccaccgatgc
     5821 cctactgcag aatatgctct gggcgctggg gcttagggag ccggtgaaac ccgaaattat
     5881 taaatggccc ccggtggaat cccccctgca agtgcaagac tggcagcaag cccgcgagat
     5941 gctgcgcagt ttcctcactc cccgcttagg gcctggggag cgcttgattc tgatggggga
     6001 agccgcctac aacgcctgca cccaagggcc gtttgactat gccgagcgcc tggcagcggg
     6061 ggacatgcaa caccccagca acccctgtgt gctgctgccg agcctggcgc aaatactcag
     6121 cgagccacag cttaaggccc gcatctggcg ccaactgcaa caggtttaac cgtggcacct
     6181 tttaccgcct gctccctgcc gtctccacta ggtcccctgc acttttgtgc cttgggcctg
     6241 gagcatctca acgaaatcga tcagctggaa caggccagca gcccccaccc ctggagccgc
     6301 gccaatatcg caagctccct gggcagcagc catagggggc tggcattgcg ctgggagggg
     6361 aaattattcg cctatgggtt tgtcagcatc gcctcagttg aggcggagct gttgatcatt
     6421 accgtggcga aaaagcgaca gggccaaggg tttggccgcc tattgctcgc caccatcatc
     6481 gagcaactga aggggcgcgt gagtgaaatg ttcctggagg tgcgcccttc caacacagcc
     6541 gctctggcgc tgtatgaatc cttagggttc aaccaggtgg gcgtgcggcc ccgctattac
     6601 cccggcccta gaggggggga ggatgcgtgg gtgatgggct tgtgcttaga ggctgagtaa
     6661 atcagcgctg taaaaataat tagtcttcac cctgcagcgt caacaccaac cggcgggcgc
     6721 cgccatgatc gcggtgctca cacagataaa caccctgcca ggtacccagc tgcaaacgcc
     6781 cattggcaac aggcaacgaa atactgctgc ctaataaact ggctttgata tgggcgggca
     6841 agtcatccag gccttcgtcg ttatgttcgt aggcgctaga gctttcggga attaagcggt
     6901 taaacacggt taaaaaatcc cgccgcacac tggggtcggc attttcatta atgcataaag
     6961 ctgccgatgt atgctggata aaaacatgta ataaacccac ccgcaggcga gttatttccg
     7021 gcaaggcatc ggtaatttcc tgggtaatta aatgaaagcc gcgcgggcgc gccttaaggc
     7081 ttatgtgttt ggataaccac acggttaaaa accttctgaa tgggtgtcgc agctggtgac
     7141 ccaccagcgc gtaggatcaa taaaatgcgc ttgtaattca ctgtctttta ctgctacacc
     7201 aaataaatgg gcatcctcac gaaaggtaaa ccccatgggg atcatcaagg tttgccacag
     7261 ttcgttatgg gcggtaaagc agactaaatc cacactggcg aggcttgcga tttccaacag
     7321 cgcaaccacc aacgcttgta gaccttttaa attattggcg gcggtaaata aatcgtcgat
     7381 atgtaaatgg gacttaccct tgatgtggta caccgcgtaa ccggctaata agccctcatg
     7441 aaataaaccc agggcattat attggcggcg cggattggtt aaaaaacgcc actgcagaaa
     7501 atccgccgag cgctggcgca aaacccacag ggtgtcttta tagctgtcca ttagggcgtc
     7561 aagctgctgg gcatgcaaag taaaatttag tacaggcgct atttgccagg gctttttttt
     7621 gccgcgcccg agccatttgc gcgtgaggtt taaaaccgtg tccacacatt tggcggcaat
     7681 gggtaataac agcggatgaa cttttttatg caaataacgc gagctgtgca ataaatattt
     7741 cc
//
LOCUS       DAOQGS010000145         7741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4531_length_7741_cov_5.918813, whole genome shotgun sequence.
ACCESSION   DAOQGS010000145 DAOQGS010000000
VERSION     DAOQGS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_7741_cov_5.918813"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKZ52_07205"
     CDS             <1..824
                     /locus_tag="PKZ52_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNG59598.1"
                     /translation="CHLHPAPKLLLVSGGSGVTPMLSMTRWLCDTASNVDIVFLHVAK
                     TALDIVARDELEMLAARHANLKLVFSVTQDKAPSSWAGWRGRISADLLNLAVADFKER
                     RVFVCGPEAFMESTKEVLLNLNFPMHHFHEESFGGPKAKSAATSAAVSIEEAAPKFGL
                     KALLQAVTPARTGGDATGGSKKAAAAPQVHFIKSANKSPITPGNSVLELAEQCGMQLP
                     FGCRQGMCGACKQKLLKGQIATEGYNDKVLNAQDKSAGFILTCIAKPQGDIEIDA"
     gene            complement(915..1811)
                     /gene="htpX"
                     /locus_tag="PKZ52_07210"
     CDS             complement(915..1811)
                     /gene="htpX"
                     /locus_tag="PKZ52_07210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNG59599.1"
                     /translation="MMRIVLFLLTNLAVMLVAGITLNLLGVSHFVERQGMNFTSLLIY
                     CAVFGFTGSFISLFLSKSMAKMSTGTQIITTPQDADEAWLVQTVQELADKAGIAMPEV
                     GIFPSQDANAFATGWNRNAALVAVSAGLLQRFSRDEVRAVMAHEIGHVANGDMVTLTL
                     IQGVVNTFVMFFARIVGYAVDSVLRRGNQSSYSVGFGYYIGSMVAEVVFGIVASMIVA
                     WFSRLREFRADQAGAQFASKHGMISALQRLQFESQQHTDSMPAALSAFAISSGIKGKF
                     FALLATHPPLEVRIQALSQMPV"
     gene            complement(2057..3400)
                     /gene="mnmE"
                     /locus_tag="PKZ52_07215"
     CDS             complement(2057..3400)
                     /gene="mnmE"
                     /locus_tag="PKZ52_07215"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNG59600.1"
                     /translation="MATTIAAVATAPGRGGVGVVRLSGPLCLPIAEQLLGFTPKNRYA
                     HYCPFLDAQGKAIDAGIALFFQGPHSFTGEDVLELQAHGGPVVLDMLLERALELGACL
                     AKPGEFSERAFLNDKMDLTQAEAISDLIAASSRQAAQQAINSLQGAFSKEVHQLVEAL
                     IGLRMYVEAAIDFPEEEVDFLSDGKIAADLADLLHQLQQLLHKTKQGRLVREGMKIVL
                     AGKPNAGKSSLLNALTERDSAIVTPIAGTTRDPLREHIHIDGMPLHLVDTAGIRPSDD
                     LVEQIGIDKAWQEIRSADRILLLLDASEDHSDLVQQWPELFGNPDLQAKLTLILNKID
                     LVEQQPEQLPAPTIALSAKHQLGIDGLKTHLKHIMGFEGVTEGSFSARRRHLEALEQA
                     RFYLLAGQQQLQDYRAGELLAEDLRQAQEALNQITGAFTSDDLLGRIFGSFCIGK"
     gene            complement(3456..5129)
                     /gene="yidC"
                     /locus_tag="PKZ52_07220"
     CDS             complement(3456..5129)
                     /gene="yidC"
                     /locus_tag="PKZ52_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNG59601.1"
                     /translation="MKTDWLRKIILGGIIVIGFVLFIRWNEFQEKHHAAQSQLPAATT
                     ATATETAQTTTPKGNEEFALPAPVDSGKQEADKALIKTAVVSVRTDSLWVEIDTQGGD
                     IRKVSLPKNFAELNKPDAPFVLLDNTASRTYIAKSGLAGPNGTDSASGRPQFSVSADT
                     FSLPEGQNELAVDLTLVQPEAQITKRFIFTRGSNLIKVKYLIKNSSDQPWQASLVGSI
                     KRDAYNPSPAQLFAMNPFIGAATSTTEKNYHKLTFKDIAENGFKHEKEGGWLALVQHY
                     FLTAWIPPQNSKNAFNFQKVKDEDLYILSFTSAPTLISAHSEGSIEAGFYAGPKDMDI
                     LETIAPHLDLTMDYSWLWWLAKPMFKTLDIIHGVVQNWGVAIILLTLLIKAILFYPSA
                     ISYKSMARMRKVQPKMQALKERYGDDRQKMSQELMKLYKDEKVNPVSGCLPMLLQMPV
                     FLALYWVLMEAVELRQAPFCLWIQDLSVKDPYFVLPLIMGATMYFQSKLNPTPPDPMQ
                     AKMMQMMPIFFTFLFVFFPAGLVLYWVVNNSLSILQQYVITKQIEKSNK"
     gene            complement(5146..5508)
                     /gene="yidD"
                     /locus_tag="PKZ52_07225"
     CDS             complement(5146..5508)
                     /gene="yidD"
                     /locus_tag="PKZ52_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNG59602.1"
                     /translation="METHQQTRHTNSHRVNALIQQALQGVIRLYQVGISPWLGKNCRF
                     YPSCSCYTHEAIAIHGPWRGLWLGICRIAKCHPWHPGGVDLVPVKPAVPKKLRLICRI
                     NKITDKKAPHKHSPSTRT"
     gene            complement(5454..5897)
                     /gene="rnpA"
                     /locus_tag="PKZ52_07230"
     CDS             complement(5454..5897)
                     /gene="rnpA"
                     /locus_tag="PKZ52_07230"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNG59603.1"
                     /translation="MSHLLRSSVSIAPNTLSGSKPLSRLLFTKQQRLLEAKEFSALFA
                     DAPIRASHPCFLLLCNFNGTEQARLGLVIAKKQIKKSHDRNRLKRLIRESFRLQQHKL
                     PLIDAIVLARKGADALSNSEIQVILHGLWKRISKRATQTPTGSTL"
     gene            complement(5921..6055)
                     /gene="rpmH"
                     /locus_tag="PKZ52_07235"
     CDS             complement(5921..6055)
                     /gene="rpmH"
                     /locus_tag="PKZ52_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNG59604.1"
                     /translation="MKRTFQPSVLKRKRTHGFRARMADRHGRKVLARRRAKGRKQLVA
                     "
     gene            6366..>7741
                     /gene="dnaA"
                     /locus_tag="PKZ52_07240"
     CDS             6366..>7741
                     /gene="dnaA"
                     /locus_tag="PKZ52_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNG59605.1"
                     /translation="MTSPFHVWEFCSGQLQTELPAPQFNTWIRPLKLDEAGDVLRLLA
                     PNRFVLDWVNDKYLSRIRDLVAQGGQYFKVEVVVADKPSAAFTRAVATADAPAPIARS
                     APAATSEFIPSQPLAIELDQNDGIEALGELEEDLSPLTPKTTGSHSNASNKRNNKDDI
                     EGGLAHRTYLNPAFTFKTFVEGKSNQLGLAAAIQVAENPGGSYNPLFIYGGVGLGKTH
                     LMHAVGNALIAKKPNAKVVYLHSERFVADMVKALQLNAINDFKRYYRSVDALLIDDIQ
                     FFAGKERSQEEFFHTFNALLEGGQQIILTCDRYPKEISGLEERLKSRFGWGLTVAVEP
                     PELETRVAILMKKADQAHMNLPHDAAFFIGQRIRSNVRELEGALKRVIANAHFTGRDI
                     SIDLVRESLKDLLALQDRLVSVDNIQRVVAEYYKIKVADLLSKRRSRSVARPRQVAMY
                     LAKDLTNH"
BASE COUNT         2013 a         1918 c         1881 g         1929 t
ORIGIN      
        1 cctgtcacct gcatccggcg ccgaaactgt tattagtctc cggcggcagc ggggtaacac
       61 ccatgttgtc aatgacgcgc tggctgtgtg ataccgcctc caacgtcgat atagtatttt
      121 tgcacgtagc taaaaccgct ttggatatag ttgcccggga tgaattagaa atgttagcgg
      181 cgcgccacgc taatttaaaa ttggtcttta gcgtcacgca ggataaagcc cccagcagct
      241 gggccggttg gcgtgggcgc attagcgctg atttattaaa cctcgcggtg gctgacttta
      301 aagagcgccg agtttttgtc tgcggcccgg aagcttttat ggaatccact aaagaggttt
      361 tactcaacct taatttccct atgcaccatt ttcacgagga gagttttggc gggcccaaag
      421 caaaatcggc cgcaacctct gcagcagtat caattgaaga agcggcccct aaattcgggc
      481 taaaagcgct cttgcaggca gtaacacctg cccgtacagg cggcgatgcc accggtggta
      541 gtaaaaaagc tgctgccgca cctcaagtgc attttattaa atcggccaat aaaagcccta
      601 ttacaccagg caatagcgtg ttagaacttg ccgagcaatg cggcatgcaa ttgccttttg
      661 gctgtcggca aggtatgtgc ggcgcctgta aacaaaaatt gcttaaaggc caaattgcca
      721 ccgagggtta caacgacaaa gttcttaatg cgcaggataa aagtgccggt tttattctta
      781 cttgtattgc caaaccccag ggcgatattg agattgatgc ctaacaaccg tcctagcagt
      841 ttgttgaaat acctaggcgg cgacgcgctg gcacagtagt atttcaacaa gctgctagaa
      901 cggttgttac aagatcacac cggcatctga cttaacgcct gaatgcgcac ctctaaaggc
      961 gggtgcgtgg ccagcagcgc aaagaatttg ccctttatac ccgatgaaat tgcaaaggca
     1021 ctgagcgctg caggcataga gtctgtgtgc tgctgggatt caaattgcag gcgctgcagg
     1081 gcggaaatca tgccgtgttt gctggcgaat tgggcgcctg cttgatccgc gcgaaattcc
     1141 cgcaggcggg aaaaccaggc tacgatcata ctggccacaa tgccaaacac cacttcagcc
     1201 accatgctgc cgatgtaata accgaagccc acgctgtagg aagactggtt gccccggcgc
     1261 aacacactgt ccacggcata acccacaatg cgagcgaaaa acatcacaaa ggtattgacc
     1321 accccctgaa ttaacgtgag ggtcaccata tcgccattgg ccacatggcc aatttcatgg
     1381 gccatgaccg cgcgcacctc atcgcggcta aagcgctgta acaggccagc gctcacagcc
     1441 actaaggcgg cgttgcggtt ccaaccggtg gcaaaagcat tggcatcctg ggaggggaaa
     1501 atacctacct ccggcatggc aatgccggct ttatccgcca attcctgtac ggtttgcaca
     1561 agccaggctt catccgcatc ctgaggcgta gtaataatct gggttccagt ggacatcttc
     1621 gccatggatt tggacaaaaa tagcgaaata aacgagcctg taaaaccaaa caccgcgcag
     1681 tagattagca gtgaggtgaa attcatgccc tgccgctcaa caaaatggct gacgcctaaa
     1741 agattcagcg taatcccagc caccagcatc acagctaaat tagtcagtaa gaataaaact
     1801 atccgcatca tataaaatcc gcctgtgtga attacttgct aacaatgtgg ggcagggtcg
     1861 aggggtttca agcaataaaa acaataactg ccatcacgca accctacagg tattattaac
     1921 ccggcggtgt aaaccgccgg gagctgcgct aaaacaagaa aatccgcgca ttttcgtagg
     1981 tttagcgcag cgcaggcaaa atagattagg acgatctatt ttgccgccgg gttaataatc
     2041 cggcttaaga agccaattac ttgccaatac aaaaagagcc aaaaatacgg cccaataaat
     2101 cgtcgctggt gaaggcgcca gtgatttgat tcagcgcttc ctgcgcttgc cgtaaatcct
     2161 cggctaagag ttcacccgcc cgataatcct gcaattgctg ttgcccggct aacaaataaa
     2221 aacgcgcctg ttctaaggcc tccaaatggc ggcggcgggc gctaaaactg ccttcggtaa
     2281 ccccttcaaa ccccatgatg tgttttaaat gggttttaag tccatcaatt cccaattggt
     2341 gttttgccga cagggcaatt gtgggggcag gcaattgctc aggctgttgc tcaaccaaat
     2401 caattttatt gaggataagt gtcaatttag cctgcaaatc ggggttgccg aacaactctg
     2461 gccactgttg caccaaatcg ctgtggtctt cgctggcgtc taataacaat aaaatgcgat
     2521 ccgccgagcg aatctcctgc caggctttat ctatgcctat ttgctctacc aggtcgtcac
     2581 tggggcgaat accggcggtg tccaccaaat gcagtggcat gccgtcgata tgaatatgct
     2641 cgcgcaaggg gtcgcgggtg gtaccggcaa tgggcgttac tatggcgctg tcccgctcag
     2701 ttagggcatt taataagctg gatttacccg cattgggttt accggctaaa acaattttca
     2761 tgccttcacg cactaaacgg ccctgtttgg ttttgtgcaa taactgctgc agttgatgta
     2821 ataaatcggc caagtcggcg gcaattttac cgtcggataa aaaatccact tcctcttcgg
     2881 ggaaatcgat ggcggcctcc acatacattc gcagcccaat taatgcctcc actaactggt
     2941 gtacttcttt ggaaaaggcc ccttgcaagc tatttatagc ttgttgagcc gcttggcggg
     3001 aactggcggc aattaaatcg ctgatcgcct ccgcctgagt taaatccatt ttatcgttta
     3061 aaaatgcccg ttcagaaaac tcccccggtt tagcaaggca ggcgcccaat tcaagcgccc
     3121 gctccagcaa catatccaac accaccggac cgccgtgggc ctgtaattcc agtacatctt
     3181 caccggtaaa cgaatgcggc ccttgaaaaa ataatgcgat acctgcgtcg atggccttac
     3241 cctgcgcatc caaaaacggg caatagtgag cgtaacgatt tttaggcgta aaaccgagta
     3301 attgctcagc gataggcagg cataaaggcc cggataaacg cactacgccc acaccgccgc
     3361 gcccgggggc tgtagctacg gctgcaattg ttgtggccat aaatcctcac caataaaaaa
     3421 gggcgacaag tcgccctttg ttttttcact tacttttatt tattcgactt ttcaatttgt
     3481 ttggtaatca catactgctg cagaatcgac aagctgttgt ttaccaccca atacaacacc
     3541 aaaccggccg ggaaaaacac aaacaaaaag gtaaaaaata tcggcatcat ctgcatcatt
     3601 ttcgcctgca taggatcggg cggcgtgggg ttaagcttgc tttgaaaata catggtcgcg
     3661 cccataataa gcggcaacac aaagtagggg tccttcactg ataggtcttg aatccacaaa
     3721 caaaacggcg cctggcgcaa ctccacggct tccattagta cccaataaag ggccaaaaat
     3781 accggcattt gcagcaacat gggtaaacaa ccgctgaccg gatttacctt ttcatcctta
     3841 taaagcttca taagctcttg agacattttt tgccggtcat ccccatagcg ctcttttaac
     3901 gcctgcattt tcggctgtac tttacgcatg cgggccatgg atttgtaact aatggccgag
     3961 gggtaaaaca aaatggcttt aatcaataag gttaataaaa taatcgccac accccagttt
     4021 tgcaccacgc cgtggataat atccaaggtt ttaaacatgg gttttgccag ccaccacaac
     4081 cagctgtaat ccatggtcaa atccaaatgg ggcgcaatgg tttctaaaat atccatatcc
     4141 ttggggccgg cgtaaaagcc cgcttcaatg ctgccttcac tgtgagcact aatcagggta
     4201 ggggcgctgg taaaacttaa aatgtacagg tcttcatctt taactttttg gaagttaaag
     4261 gcgtttttac tgttttgcgg cggtatccag gcggttaaaa aatagtgctg taccaatgcc
     4321 aaccaaccgc cttctttttc atgtttaaaa ccgttttcgg caatgtcttt gaaggtgagt
     4381 ttgtggtaat tcttttcggt tgtgctggtc gccgcgccta taaacgggtt catggcaaac
     4441 aactgggcag gggatgggtt ataggcatcg cgctttattg aacccactaa acttgcctgc
     4501 caaggctgat cagaactatt ttttattaag tatttaacct taattaaatt actgccgcgg
     4561 gtgaagataa accgcttggt aatctgggct tctggctgta ctagggttaa atccaccgcc
     4621 aattcatttt ggccctcagg taagctaaac gtatcggcgc taacgctaaa ttgtgggcgg
     4681 ccactggcgc tatcagtgcc gttggggcca gctaagccac tttttgcaat gtaggtgcgc
     4741 gatgcagtgt tatccagcag cacaaaaggt gcatcgggtt tatttagttc ggcaaaattt
     4801 ttgggcagcg aaactttgcg aatatcaccg ccttgagtat caatttccac ccataggcta
     4861 tcggtgcgta ccgacacaac agccgtttta attagcgctt tatcagcctc ttgtttgccg
     4921 ctatccacag gtgctggcag agcaaattct tcattgcctt taggggtagt agtctgcgcc
     4981 gtttcagttg cggtggcagt tgtggcagcg ggtagctggc tttgagccgc gtggtgtttt
     5041 tcttgaaatt cgttccagcg aataaacagg acaaacccga tcactataat gccaccaaga
     5101 attattttgc gtagccaatc cgtcttcatg gatataaacc gttatttaag tgcgtgttga
     5161 aggagaatgt ttatgaggcg cttttttgtc agttattttg ttaattctgc atattaaccg
     5221 cagctttttg ggcactgcgg gtttaaccgg cactaagtcc accccacctg ggtgccaagg
     5281 gtggcattta gcaatgcgac aaatacccag ccaaagcccg cgccatgggc cgtggattgc
     5341 aatagcttca tgggtataac aagaacacga ggggtaaaac cgacagtttt tgcctagcca
     5401 aggactgata cccacttggt acaagcgaat cacaccctgc agcgcctgtt gaattaaagc
     5461 gttgacccgg tgggagtttg tgtggcgcgt ttgctgatgc gtttccataa accgtgcaaa
     5521 ataacctgga tttcgctatt agaaagagca tcggcccctt tgcgggcgag cactatagca
     5581 tctattaaag gcagtttgtg ctgctgcagg cggaaggatt cgcgaattaa ccgcttaagg
     5641 cgattgcgat cgtgggattt tttaatttgt tttttggcaa ttaccaaacc gagccgtgcc
     5701 tgttctgtgc cgttaaaatt acacagcaat aaaaaacaag ggtggcttgc gcgaatgggc
     5761 gcgtcggcaa atagagcact aaactctttt gcttctaata ggcgctgctg cttggtaaat
     5821 aaaagacgtg atagcggctt agagccgctt aaagtgtttg gtgcgatgct cacagacgag
     5881 cgcaataaat gggacaccaa acaccttcag cgattgtggc ttacgccact aattgcttac
     5941 gacccttagc gcgacggcgg gccagtactt tacggccgtg acggtcagcc atacgagcgc
     6001 ggaaaccgtg agtacgctta cgctttaaaa cgctgggttg gaatgttctt ttcatgatgt
     6061 cgatacctga ttcgatgggc aactattttt agcaacacta gggtaaaagg tcgccagaaa
     6121 aagaggcgcg attctataga aaggccgccg taaaggcaac gtaaatttct cataaatcca
     6181 cggcttaaca cgattagaaa aggtcatttt attcaggatt tatctacagg tttatcacag
     6241 cttattctca aatctagcga ttcccacaag tgtgtttaac acgccgggta atcaactgat
     6301 tgctgtggat aaccttgtgg atgcaggtta aactgccagg cattttttta agcatgcagg
     6361 ccatcatgac ttcacccttc cacgtctggg aattttgtag tgggcaatta caaactgaac
     6421 tgccagcccc tcaatttaat acctggattc gcccattaaa gctggacgag gctggagatg
     6481 ttttgcgcct gttagcaccc aaccgatttg ttttggactg ggtgaatgat aagtacctgt
     6541 ccagaattcg cgacttagta gcccagggcg gccagtactt taaagttgaa gtggtggtag
     6601 cggataaacc ctcggcggcc tttactcggg cggtggcaac agctgatgct cccgcaccta
     6661 ttgctcgctc tgcacctgcc gccacttcag aatttattcc gtcacagcca ctggctattg
     6721 aactggatca gaacgacggc attgaggccc taggtgaact ggaagaggat ctaagcccgt
     6781 taacacctaa aactacaggc tctcattcca acgcttctaa taaaagaaac aataaagatg
     6841 atatagaagg tgggctggcc catcgcacct atttaaatcc tgccttcacc tttaaaacct
     6901 ttgtggaagg taaatctaac cagttgggct tggccgccgc tattcaggtg gcggaaaatc
     6961 cgggcggctc ttacaaccct ctttttatat acggcggtgt gggcctgggt aaaacccacc
     7021 taatgcacgc tgttggcaat gccttaatag ccaaaaagcc caatgccaag gtggtgtatc
     7081 tgcactcgga gcgctttgtg gccgatatgg taaaagccct gcagctaaat gccattaacg
     7141 atttcaaacg ctactaccgc tcagtggatg ccttgctcat cgatgacatc caatttttcg
     7201 ccgggaaaga gcgctcccag gaggagttct tccatacctt taatgcgttg ttggagggtg
     7261 gccagcagat tattctcacc tgtgatcgct atccaaaaga aatcagtggc ttagaggaac
     7321 ggcttaaatc tcgtttcggc tgggggctaa cggtggcggt tgaaccgccg gagctggaaa
     7381 ccagggtggc gattctgatg aaaaaggccg atcaagccca tatgaatctg ccccacgatg
     7441 cggctttttt tatcggccaa cgcattcgct ccaacgtgcg cgaacttgaa ggcgccttaa
     7501 aacgagtcat cgccaacgca cattttaccg gccgcgatat ttccattgat ctggtgcgtg
     7561 agtcgctaaa agatttatta gccctgcaag atcgcctggt gagcgtagat aatattcagc
     7621 gggtggttgc cgagtattac aaaataaaag tggcggacct gttatccaaa cgccgcagcc
     7681 gctcggtggc caggccccgg caggttgcca tgtatctggc gaaggatctt accaatcaca
     7741 g
//
LOCUS       DAOQGS010000146         7652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4599_length_7652_cov_5.292221, whole genome shotgun sequence.
ACCESSION   DAOQGS010000146 DAOQGS010000000
VERSION     DAOQGS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4599_length_7652_cov_5.292221"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKZ52_07245"
     CDS             <1..442
                     /locus_tag="PKZ52_07245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNG59606.1"
                     /translation="KALKKGPVVVYFYPSAYTRGCNVQAHSFSVNTEKFAAAGASVIG
                     VSLDSVERLKEFSADPEFCAGKLPVASDAQGLTAKAFDLAVRAAEAGKVDSRGQTIDH
                     GFAERTTFVITPNGKIAATIGGLAPELNVKQSLEFVESLAANKK"
     gene            664..1686
                     /locus_tag="PKZ52_07250"
     CDS             664..1686
                     /locus_tag="PKZ52_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNG59607.1"
                     /translation="MARVKRYAYTHLLSAVRERLQAAGVPQTQAEVEAELMVEADLLE
                     VPSHGVRMLPGLLAALESGRLAPQANFTSERNFGAISVINCGAGPGRFASLHAMHMAM
                     ESARSFGVGVCLAKNTSHWGRAHAYASRAAQAGFIGICTTNAMPTMAIGGAKKPVIGN
                     NPLAIGIPRQERACPIVLDIAMSQAAVGKVNTWLREGKTPPNNWGINAAGEPTNDAAE
                     ILKGAVLPFGGHKGAGLAVMLELITAALAGAAFTQEIAAADNTGLDTGASKLFIALHP
                     SAFIDDEQFDRRVNDFLQYLDINAAPFLYPGARGWQAQAINRVQGVPIHQEIVQQLAR
                     AGLILE"
     gene            complement(1894..2820)
                     /gene="cysK"
                     /locus_tag="PKZ52_07255"
     CDS             complement(1894..2820)
                     /gene="cysK"
                     /locus_tag="PKZ52_07255"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNG59608.1"
                     /translation="MRYDSILGTIGNTPVVRINHLFRPDIEVWMKIERFNPGSSIKDR
                     IALAMIEDAERKGLLTPQTTIIEPTSGNTGIGLALVAAVKGYRLILTMPESMSIERRK
                     IMQAYGAQLVLTPKEQGMGGAINKARELQQEYPNSWIPQQFNNPANTEIHRTTTAQEL
                     LNDFPEGIDYIITGVGTGGHITGCSEVLKHYFPQLKTYAVEPEKSPVISGGQKGLHRL
                     QGIGAGFIPEILNTATLDGIIQVSETDAFEQARAVAKKEGIFVGISSGATLAAVAQKQ
                     HEFAPGSRVVIFSYDTGERYLSIEDLFLTQPA"
     gene            complement(3112..4287)
                     /locus_tag="PKZ52_07260"
     CDS             complement(3112..4287)
                     /locus_tag="PKZ52_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNG59609.1"
                     /translation="MSEDSKQPMVLSLEGGNAQPLSVRAKALVFSDPVSARLLEYIER
                     IAPSEAPVLIGGETGTGKELVARHIHLLSGRKGPFLAVNCGAISDQLVESELFGHEAG
                     SFTGAVGKREGWFEAANGGTLFLDEIGDLPLAMQVKLLRVLQEGEIVRIGSRKSIRVD
                     VRLVAATNVDLDFAVNAGHFRRDLLFRINIAQVNLPPLRERPGDILPLADFFLKQYCQ
                     RMGYEIPQMSQEASNLLLNHFWPGNIRELENVVHFALLVAGEGRIEMEHIKVAGGWKA
                     GLEHTKPIDITINGAAQGVIPIAAAPTNQESPVDIIKNQLRHLFRDNSPYGHFDKLEE
                     VIVTEAFQHAKRNQVHTAALLGISRNVMRTLLKRHGFIHDSPVFDLANSNFPRENIN"
     gene            complement(4483..5484)
                     /locus_tag="PKZ52_07265"
     CDS             complement(4483..5484)
                     /locus_tag="PKZ52_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNG59610.1"
                     /translation="MKRFAKQAVLLSAVVLASSAAWAKEVTLLNVSYDPTRELYIEYN
                     AAFAKYWKTKSGDDVKVEQSHGGSGSQSRAIIDGLRADVATLALAADVDALAKKAQLL
                     PENWITRLPNNSAPYTSTIVFLVKKGNPKKIKDWDDLVKPGIGVITPNPKTSGGARWN
                     YLAAWGYGKHKLGSDAAAQDFVAKLYKNVPVLDSGARGSTTTFAQRGVGDVLLTWENE
                     AHLALKEFGADKFDIVIPSLSVLAEPSVAVVDKNAEAKGNATLALEYLKFLYSKEGQT
                     IAAKNYYRPRDAEVAKAYAKNFPALQTFTIDEFGGWAKAQPEHFGDGGIFDKIYTNK"
     gene            6479..7468
                     /locus_tag="PKZ52_07270"
     CDS             6479..7468
                     /locus_tag="PKZ52_07270"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNG59611.1"
                     /translation="MSMGTQFMRATGKHFALTLISLVATYAVAASAQVASAPLQPIAG
                     VIAEGTSVEFIKDGFEGTEGPIALPDGSLIFTETRANRITQIKPDGTVQPFLQNTNGA
                     NGLAFNKQGELVAVQNLKPQVGVIYPADKAKNWVSEFEGKGFQRPNDITINKRGGVYF
                     TDIGVVPKEPNGEPARPGVYYLTSDGAIKKVIGNVERPNGVLLSADEKTLYLANTAGE
                     YVLSYPVLGDGRLGPARQFAKLGGYEKNEQGVLSSGADGLAIDNRGRLYVASNLGIQI
                     FDAKGRALGVIPLPQKPQNLAFAGADKKTLYVVGRGVAYKIAVLTPGYLGRAK"
BASE COUNT         1882 a         1755 c         1919 g         2096 t
ORIGIN      
        1 aaaagcgctc aaaaaaggcc cggtggtggt gtatttttat ccctccgcct atacccgtgg
       61 ctgcaatgta caagcccaca gcttttccgt taacactgag aaattcgccg ccgctggcgc
      121 cagtgttatt ggtgtttcgc tggattcggt ggagcgctta aaagagtttt ccgctgatcc
      181 tgaattttgt gccggaaaat taccggttgc atcggatgct caagggctta ctgccaaggc
      241 atttgattta gctgtgcgtg cggcagaggc cggtaaggtt gatagccgtg gccaaacgat
      301 tgaccacggt tttgcagagc gcacgacatt tgttataacc cccaacggta aaattgctgc
      361 cacaattggc ggcctagcgc cggaattaaa cgtcaagcaa agcctggaat ttgttgaatc
      421 cttggcggcg aataaaaaat aaatccatcg attgaagcag gcgcgcgaag aagctccgcg
      481 cgttttgcga ctgcattatg tttgtatttt agaacacgcc ccattctggt gtgttctaaa
      541 atacaaccag cccttaccct atgggcaata caccttcgcc cgcccgcatt ttaaatccat
      601 tgttaaaata ggcagctgag ccattgttgc ggtatcctcc ggctttttgc tgaggttata
      661 aaaatggcca gagttaaacg ctatgcctat acccatttgt tgtctgccgt cagagagcgc
      721 ctgcaagcgg ctggtgtacc ccaaacgcag gctgaggtcg aagccgaatt aatggtggag
      781 gccgatttat tagaggtgcc atcccacggc gtgcgtatgc taccaggtct attagcggca
      841 ctggagtcgg gacgtttagc gccacaagcg aactttacta gcgagcgaaa ttttggtgcg
      901 attagcgtaa ttaattgcgg cgcaggcccc ggacggtttg cctctttaca tgctatgcat
      961 atggcgatgg aaagcgcgcg cagttttggg gttggtgttt gtttggctaa aaataccagc
     1021 cactgggggc gtgctcacgc ctatgcaagt cgcgccgctc aagccggttt tatcggtata
     1081 tgtactacca atgccatgcc gaccatggca atcggcggtg ctaaaaaacc cgttattggc
     1141 aataaccctc tggcaattgg tattcctcgc caggagcgag cctgccccat tgtgctggat
     1201 atagccatga gccaagctgc agtcggcaag gtgaatactt ggctgcgcga aggaaaaaca
     1261 ccgccgaata attggggaat taatgcagcg ggagagccta ctaacgatgc cgccgagatt
     1321 ttaaaaggcg ctgtgttacc ctttggcggc cataaggggg caggccttgc ggtaatgttg
     1381 gaattaataa ccgccgcctt agctggtgcg gcgtttaccc aggaaattgc ggcggctgat
     1441 aacaccggct tagatacagg cgcaagcaaa ctatttatcg ctctgcatcc aagtgcgttt
     1501 atcgacgacg aacaatttga tcggcgtgta aacgattttt tgcagtattt agatattaac
     1561 gcggcccctt ttttataccc gggtgcgcgg ggctggcagg ctcaggcgat taatcgtgta
     1621 cagggcgtgc ccattcacca agagatagtg caacagttgg cgcgggcagg gcttattctt
     1681 gagtaacatt gagagccgga tttttagtcg gcgccgcttg cagcctattg aaattccctg
     1741 gcggcaacat tttgtgaatt ttaatgctgt gagagcaatt aaaataatat ttcaacaggc
     1801 tgttaagagg tgctttcaaa atacaggcgg gcttcttgcc cgcgcggatg ctcgaaacta
     1861 tcagttaact ataggcttct aaggtgatta aaattatgcc ggttgagtta aaaacaaatc
     1921 ctcaatagac aagtagcgct cacctgtatc gtagctaaaa ataactactc ggctgccagg
     1981 tgcaaattcg tgttgttttt gtgctaccgc cgccaaggtg gctccagatg agatgccgac
     2041 aaatatgcct tctttttttg ctacggcgcg ggcctgctcg aacgcatctg tctcgctcac
     2101 ttgaattatg ccgtctaagg tcgcggtatt gagaatttcg gggataaacc ctgcacctat
     2161 accttgtaag cgatgcaaac ctttttgccc accgctaata acgggagatt tctcaggttc
     2221 aacagcatag gtttttaatt gcgggaaata atgcttgagc acttcgctac aaccggtgat
     2281 atgtccgcca gtgcccaccc cggtaattat gtaatctatt ccttctggga agtcatttaa
     2341 taattcttgg gcggttgttg tgcggtgaat ttcagtgttg gcggggttgt taaactgttg
     2401 tggaatccag ctattggggt actcctgctg cagctcacgg gctttattga ttgccccgcc
     2461 catgccttgt tctttggggg ttaataccaa ttgcgcgccg taggcctgca taattttgcg
     2521 ccgctcaatg gacatggatt ccggcatggt taaaatcagc cgatagcctt tcaccgcagc
     2581 taccaacgcc aaacctatgc ccgtattgcc tgatgtgggc tcaatgatag tcgtttgggg
     2641 tgtcaacagt cccttgcgct ctgcgtcttc aatcatggca agtgcaatac gatccttaat
     2701 actgctgcct ggattaaagc gttctatttt catccacact tcgatgtcag ggcgaaataa
     2761 atggtttatt cgcaccaccg gagtgttgcc aatcgtgccg agaattgaat cgtatcgcat
     2821 ggtatatctc tcttgccgat gataggctca ataatgaatt gcggactaaa gtgattaagg
     2881 aacctctgaa aaattacctc actcgcccta gcgtcgttga atttggcaaa aaaatgctca
     2941 tttactgcgt gtaaactccg ctttttttgc caaattcgcc tagctagtgc ttcgccgcct
     3001 acatttttca gaggttcctt aagtatgtaa gtgcctgtta aaaacctaaa aaataaccga
     3061 atggtgctgt gtcactaatg tagtggctgg cgcttatgcg ccagccactg tttagttgat
     3121 gttttcgcgc ggaaaattac tgttggccag atcaaatacc gggctgtcgt gaataaatcc
     3181 gtggcgtttt agtagagtgc gcatgacatt gcgggatatt cccagcaggg ccgctgtatg
     3241 tacctgattg cgttttgcat gctggaaggc ttctgtcact atcacttctt ctaatttatc
     3301 aaaatgccca tagggactat tgtcgcgaaa cagatgccgc aattgatttt taataatatc
     3361 aacgggggat tcttggttgg tgggtgctgc ggcaatagga ataactcctt gcgcggcacc
     3421 attgatcgtt atgtcgatag gcttggtatg ttcaagccct gccttccaac caccagccac
     3481 ttttatatgt tccatttcaa tacggccttc gcccgccacc aaaagggcaa aatgcacgac
     3541 gttttccagc tcacgaatat tccctggcca aaaatgattc agtaataaat tactggcctc
     3601 ttgggacatt tgcggtattt cataccccat acgctggcaa tattgtttta agaaaaaatc
     3661 ggccaagggc aaaatgtccc caggccgctc ccgtaggggc ggcaggttta cctgagcaat
     3721 attgatgcgg aacaataggt cacggcgaaa atggccggca ttcacagcaa aatctaaatc
     3781 cacgttggtg gcggcaacca gtcgcacatc aacgcgaata cttttgcgcg agccgatacg
     3841 aactatttcg ccctcttgca atacacgcag taatttaacc tgcatagcga ggggcaggtc
     3901 gccaatttca tccaaaaaaa gtgtgccgcc gttggccgct tcaaaccagc cttcccgttt
     3961 gcccactgca cctgtgaatg aaccggcttc gtggccgaat aattcactct ccactagctg
     4021 gtcgctaatg gcgccacagt tgacggctaa aaatggccct ttacggccgc tgagtaagtg
     4081 aatgtggcgg gccactagct ctttgcctgt gccggtttcg ccgccaatta aaacgggtgc
     4141 ctcgcttggg gcaatgcgtt caatatattc taatagccgc gctgagaccg ggtcggaaaa
     4201 tactagggct ttagcgcgca ccgacaacgg ctgcgcattt ccgccttcta gtgatagcac
     4261 catgggctgt ttactgtctt cactcacgta taaatcctca atagctcacc cgaatatagg
     4321 agttatgagt gggtgaggtt gtaaagctgt tgggcattgt aaaattctgt tacttttttt
     4381 taaaacaata aaaatgtcta aggttatatg caaaagtgct atagaatttt gggcaaaaaa
     4441 aagcccgctg gagacgggca aaaactggag aggagtgagt tattatttgt tggtgtaaat
     4501 tttgtcgaaa attccaccat caccaaaatg ttcaggttgt gccttagccc agccaccgaa
     4561 ttcatcgatg gtgaaggttt gcagggcggg gaagttcttg gcgtaggcct tggctacctc
     4621 tgcatcgcgg ggccggtagt agtttttggc cgctatggtt tgcccttctt tactgtataa
     4681 aaacttcaag tattcaagcg ccagggttgc attgccttta gcttcggcat ttttgtcaac
     4741 cactgcaacg gagggctctg ctaatacaga cagggatggg atcacaatat caaacttgtc
     4801 tgcaccaaat tcttttaagg ctaaatgcgc ctcgttttcc caggtgagca atacatcacc
     4861 aacacccctt tgggcgaagg tagtggttga accgcgggca ccagaatcta atacagggac
     4921 atttttatag agtttggcaa caaaatcctg agccgccgcg tcgctgccca gcttgtgttt
     4981 gccataaccc caggccgcta aataattcca gcgtgcaccg ccggaggttt tcgggttggg
     5041 ggtaataaca cctataccgg gtttgaccag atcatcccaa tctttaattt ttttcgggtt
     5101 acctttttta actaaaaaca caatggtgga tgtgtagggc gcgctattgt tgggtaagcg
     5161 ggtaatccag ttttcgggga gcaattgtgc ttttttagca agtgcgtcta cgtctgctgc
     5221 caaggccagg gtggccacat cggcacgcag gccgtcaatg attgcgcggg attggctgcc
     5281 ggagccgccg tgggattgct caacttttac gtcgtcaccg gatttggttt tccagtattt
     5341 ggcaaacgcc gcattgtatt caatatagag ttcccgggtg ggatcgtaag acacatttaa
     5401 taaagtgact tcttttgccc aagccgccga gcttgccagt acaacagccg acaataatac
     5461 ggcttgtttt gcaaaacgtt tcatggtgtc tccttcaaga caatggtaaa aaaattttta
     5521 taatctgagc ctgcgccaac cctagcgcta tatattgcag tagcaagtgc aataatctac
     5581 ttttatcgac ctttgggtgc ctataggcat ttccccagcg agtattgatt tcaaccagta
     5641 gcattgctct aactccgcat tgctattttt atgccgtgat attaatgcag acaataaacc
     5701 gctattttta tgcgcgattt ctcattattg cttttgcacc tgtttaattg tcagcagtcc
     5761 ggtgcagtct ggcctgttgt tttttcatca attaggcgcc agatgctggt tgtgatacag
     5821 aggaaaaggc attgcaattt aattaagaaa gctctgatta agtttgaatg tgtaaaattt
     5881 cacaaaccca caggtgcata gctgtggctc cctcgcaggt tgcctagtgt aggagtaggc
     5941 ccgcctgcct acttaatcag gggcttcctt agcgtttggc gatagatttc ctaggcccga
     6001 ttaatcagtt gcggttggcg ccgtcatccc ggagcatcta ccgagaatgt aattggcata
     6061 atcggccaat ttattttttt ggcataaaat ttgagaaaaa actatgcaca tttaagtagg
     6121 aagcctctga ttaaatctgc aggcggggat atggggcaca tttgcaggag gcaagcctgc
     6181 gcatgagtta caaaacagta cttgtgcggt tgcacaatat tcacatattc agaccaaatc
     6241 agagcatcct aaatcaacaa agatctttgt cacatcgtcg ctggctgttt tcgccggggc
     6301 atgagagtaa ttgatgcgat gggttgccgc ttgtatgtag gtttacgctt gacgccccgc
     6361 atcaaaatca gctgcttgtg cttatggcct aaggcgtgta acgaggcatt tacttaaatc
     6421 tgttaatttc tagccgcctt ttaaccgggg cacttttcat tttttaaagg ttatagttat
     6481 gagtatgggt acacagttta tgcgcgcaac aggtaaacat tttgcgttga ctttaattag
     6541 cctggtggcc acctatgcag tggctgcatc ggctcaggtt gcctcagcgc cgctccagcc
     6601 cattgcgggc gttattgctg aaggtacttc ggttgaattt attaaagacg ggtttgaggg
     6661 cacagaaggg cctattgcat tgcccgatgg cagtttgatt tttactgaaa cccgcgctaa
     6721 tcgtataacg caaattaaac cagatggcac agtgcagcct tttttacaaa ataccaatgg
     6781 cgcaaatggc ttggcattta ataaacaggg cgaattagtt gcggtgcaga atctaaagcc
     6841 acaagttggt gtaatctacc ctgcagataa ggctaagaat tgggtgagtg aatttgaagg
     6901 taaaggattc cagcgcccca atgatattac aatcaataag cgcggcggcg tttactttac
     6961 cgatataggc gtggtgccaa aagagcccaa tggcgagccc gcacgcccgg gcgtgtatta
     7021 tttaacctcg gatggtgcga ttaaaaaagt gattggcaat gttgagcggc ccaatggagt
     7081 attgctcagc gccgatgaaa aaaccttgta tttggcgaat acggcgggtg aatatgtatt
     7141 gtcgtatcct gtgctcggcg atggacggct tgggcccgcc cgtcaatttg ctaaacttgg
     7201 cggctacgag aaaaatgagc aaggtgtgtt atccagcggc gcagacggtt tagctattga
     7261 taatcgcggc cgcctttatg tggcgtccaa tctcggaatt caaatattcg atgccaaagg
     7321 ccgcgctctg ggcgtaatcc ctctgccgca aaaaccgcaa aatttggctt tcgcaggtgc
     7381 ggataaaaag acgctgtatg tggtgggccg aggcgtggct tataaaatag cagtattaac
     7441 acccggttat ttggggcggg cgaagtaact ggcctacttt agcaggatgt cgatagtgga
     7501 aaaattctat ggagcagatt tgttttaatg caaataaaca ggtaactatt cagcatcttt
     7561 ttatatggct ggaggtaatt tccgcattga ctgggagcta tcgcgtgcgt gttttagtgc
     7621 tgtgaccccg taatccagcg cccgcagtgg tg
//
LOCUS       DAOQGS010000147         7648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4602_length_7648_cov_7.341104, whole genome shotgun sequence.
ACCESSION   DAOQGS010000147 DAOQGS010000000
VERSION     DAOQGS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4602_length_7648_cov_7.341104"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..935
                     /locus_tag="PKZ52_07275"
     CDS             369..935
                     /locus_tag="PKZ52_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59612.1"
                     /translation="MDFNEQKIFDALLDRMRACRMDADESVTFVKAAIAAKRELERAD
                     GSTSAAEEFVKTVSDVVDGALKGKAGGVTKRDLIAAGLLPGRPAEGNIPFSRCGIRVD
                     SKGMVSGFGLTTAESMLNEAGSPMIFRFWLSDGIEYEIYMDGSHTFPPGTIHQNLLGL
                     RLGMAEALLKKAVKGERITDADIDSIFK"
     gene            1985..2188
                     /locus_tag="PKZ52_07280"
     CDS             1985..2188
                     /locus_tag="PKZ52_07280"
                     /inference="COORDINATES: protein motif:HMM:NF027273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HNG59613.1"
                     /translation="MIDTAIKHFESRGEFCRAVGMTPQFLSQIEAKKRPLPPKFALKI
                     EQVTNGAIKREQLRPDIYPPSDN"
     gene            2212..2466
                     /locus_tag="PKZ52_07285"
     CDS             2212..2466
                     /locus_tag="PKZ52_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59614.1"
                     /translation="MTHSNQALIKKRTIKTMVNEFQFAAWKRFAKTLELEPQEWLRNL
                     VMEIVLAANAAEDAKLNGSNVRQFPARRASDRVIKYALAN"
     gene            2548..3204
                     /locus_tag="PKZ52_07290"
     CDS             2548..3204
                     /locus_tag="PKZ52_07290"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HNG59615.1"
                     /translation="MESIIRKIKSCLALAGSDNPHEAAAAMRQAKKLMAQHGIGESML
                     DVSRTVLNTRYARPPAWFGSLGFAVGQAFGCSVFAARRVVTFVGPTGAADIAAYCFEV
                     VLRSLEASKSAFIKRTAYLCSAPVKKQRATSYCEGFVMGAQRAVDSFAPAIDKAAKQA
                     HRDYLTEVTGSGIFSSKKKKTVEINPSLISGFQDGEQVQIRAPIHQQQQQIEFLGGRP
                     "
     gene            3201..3326
                     /locus_tag="PKZ52_07295"
     CDS             3201..3326
                     /locus_tag="PKZ52_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59616.1"
                     /translation="MKYFLFLKFLIIELLSEFDEVLAARAQFKAVEQITQSGWND"
     gene            3330..4145
                     /locus_tag="PKZ52_07300"
     CDS             3330..4145
                     /locus_tag="PKZ52_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG59617.1"
                     /translation="MNLLVAPLEALTDKLLTDTERRVLLALFSFRSKSTNVVWPSTAS
                     IAERANIGDIPRVSKITSSLADKGWLTKKKKGFTGCNEYRLTFPERLNTNLAEFTNLE
                     ELTNMDEDTNTNLAEFTKSNLANFAKCNEQSIEQSIEQTISLENSTPEKKSKPEYPAW
                     FENLLSEFPRRAGSNDKRKAYIAANARTKEGATHDELLEAVRRYRRYVTANGNLNTQY
                     VLQWATFFGPGGHIKNPWTIPLFTAQPRAAPGAGTLGFTPEDYQRGINPDGSF"
     gene            4148..5014
                     /locus_tag="PKZ52_07305"
     CDS             4148..5014
                     /locus_tag="PKZ52_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG59618.1"
                     /translation="MTDAVETDIRDYVRELVRAARRRAGFEFLGQKESECERHGKFIH
                     DEFIDKDGLVTAGVCPECHAENEKQREREESIRMQAENRARAIASKFESACIPQRFKD
                     RTLSNYEIHCDKQAAVLEFCKTYAERFNEVLRLGSSIILSGTVGTGKTHLSIGIANEI
                     ISNGYAALFSTAAGIVRRVRSSWGKNDEDESAAMNIFIMPDLLIVDEVGVQSGSDNEH
                     QILFEILNARYEACKPTILLTNLPLQDQTENGSIVRKGLKSFIGERLLDRMREGGGRA
                     FTLDWKSGRIGR"
     gene            5034..5342
                     /locus_tag="PKZ52_07310"
     CDS             5034..5342
                     /locus_tag="PKZ52_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59619.1"
                     /translation="MATEKTVKTVKTVKEIVNRIIAANEESPIAVELVDGNLRSYFAK
                     TVPAPKSKTEKPGENGRIQGVVMLMPRRDEENFLGVFHGAIGAANFKKMCFGLGLTNG
                     "
     gene            5335..5793
                     /locus_tag="PKZ52_07315"
     CDS             5335..5793
                     /locus_tag="PKZ52_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59620.1"
                     /translation="MAEEFIVNSRATLAAAHARLIDNFDKHKHTVYKVVNCEEGTLPM
                     NSLVHVWLTEFAADLAQCHPKQVTKGMLEGIKRSAKGWFYHETQEPWMVHKVYCPLTK
                     RSKTDYTSMKDWKKGEKLMFMNWLQMFAAQRGTILESKGEYLDAVKKQNA"
     gene            5802..6713
                     /locus_tag="PKZ52_07320"
     CDS             5802..6713
                     /locus_tag="PKZ52_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNG59621.1"
                     /translation="MWFKNLRIYSIEFDLDAEALAAALDAHKFVPCGNLDVSRYGFVP
                     PLGDHGESLVHVTNGCIMICAKHQEKILPGAVIKSALEEKVKEINGRGFAVSRKERQD
                     LKDEIIFSLLPRAFVKDRLDFAYIDTKEQRLIVNAASAKRAERLIHELRLAIGSLKCT
                     PFECDRGAESLLTEWVRQGYAVEPFYLGESCELVGPKDGRSITAKKHDMSSEEIGVHL
                     NFGLRVEKASLFWGEDLFFTLDAGLGIKKLDYSDAVSEQVEYRKPESAAEEFDATFFI
                     MSETLRRFVKDLAGALGGEVGSPQEGE"
     gene            6713..7003
                     /locus_tag="PKZ52_07325"
     CDS             6713..7003
                     /locus_tag="PKZ52_07325"
                     /inference="COORDINATES: protein motif:HMM:NF028096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family recombination enhancement nuclease"
                     /protein_id="HNG59622.1"
                     /translation="MKNSDRLHLSKVAALGCIACINMGHPDTPAEIHHPRAGAGMGQK
                     SPHKNAIPLCHRHHRTGGPGIAIHAGQKTWEAIHGTELELLGQVRELLGVTC"
     gene            6997..7359
                     /locus_tag="PKZ52_07330"
     CDS             6997..7359
                     /locus_tag="PKZ52_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59623.1"
                     /translation="MLKILVPFVGPSTNSIYAGVHWDKRRNQKEQAMIALSALGRINP
                     VSKPVNIEVTPYLGKGKRGYDVSNYSYTYKLIEDAMVKKGILQDDSPQFVRSVKFNAP
                     VRHSEKHSATLITIEEIN"
     gene            7356..>7648
                     /locus_tag="PKZ52_07335"
     CDS             7356..>7648
                     /locus_tag="PKZ52_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59624.1"
                     /translation="MMRFLCARQAWHDAFMTDLAAPDFATQAANVGVQKTVRGANNSI
                     VDHCERGYIIAAVHRLREVDYIAYCWGMIAYAPQGTASFAEFAAMHGFMRDAF"
BASE COUNT         1973 a         1757 c         2073 g         1845 t
ORIGIN      
        1 aattctcagg caatgcggta atttgtgtgc cgcgcagatc gagatagccg cccacgttaa
       61 ttcgcccgtt actttcagta aattcgatac cgcgtttctt tagataatct agtagttcca
      121 tttgccccac ctgcttgttc gttgctgatg ggtagatatt agtaaaacac taatgtgttt
      181 ggcaatagca aattactatt attttaaaaa taatttttgt atactggatt tgccaggagg
      241 cgagcggccg gtttggcgct caatgctgtt gattggcttt tggtgttggc agccggggaa
      301 gaccggcaag cccttcttgc ggtggggtga tacccgccca aggaggctct ttaagcgagg
      361 gggttattgt ggatttcaat gagcagaaaa tttttgatgc gctactggat cgaatgaggg
      421 cgtgcagaat ggatgccgat gagagcgtga cctttgttaa ggcggcaata gccgcaaagc
      481 gggagctaga gcgtgcggat ggctccacct ctgccgccga agagtttgtc aagactgtaa
      541 gcgatgtggt tgacggggcg ctcaagggta aagcgggagg cgttactaaa agagatttga
      601 tcgctgcagg gctcctgccc ggtcggccag cggagggaaa tatacctttc tcccgatgcg
      661 gaattagggt ggactctaag ggcatggtta gcggttttgg attgaccact gcggaaagca
      721 tgcttaacga agcgggtagc ccgatgatat tcagattttg gctgtcggat gggatcgaat
      781 acgaaatata tatggacggg agccatacat ttccgcctgg gaccattcat caaaacctgc
      841 ttgggttgcg cctgggtatg gctgaggcgc tacttaaaaa ggcggtcaaa ggcgagcgga
      901 tcaccgatgc tgacattgac agcatcttca agtgatgggt taacggcatg ttaaactggc
      961 gccgcccctt agcaataggg ggcaacctaa ataactctgc cgcatggtag ccccaggcaa
     1021 tgcgcatggc tggtgtgagg ttcgagccct ccggcagagt tattcaggtt gtgatcgccc
     1081 cgcaggggat gagcacgagc agcgaaaccc ccagtcgtgc cggggagggc cggttatgcc
     1141 ggtgcggctg ctgcccgtga aattcgggcc acaacttcca aatttgcccg cttggaaaaa
     1201 gaaaacccca ggccattgct gacctggggc tttgttagag taagccagcg atgtgcaagg
     1261 taataaccag ccgagttatg gcaattatta tctttgcaac atcaatgttt acccgaatga
     1321 caatcttctt gttcacggga taaaccccta aataagttga ttgccaggtc gacccggcaa
     1381 ccacgtttta ttgtgggcca gttagcttaa cgtcgttcgc cgcgacgcct ggagttactc
     1441 gagattcccc caggattaag cgaccgggct aggccgcatt cggggattgc cccaccggtt
     1501 tgcgtagttg tatcgctacc agcgcccccg tcaccgacga gacgcaaacc ggtgatcccc
     1561 tttttgcgat aggcgcaggg cgtatagcct gtaaggtgcc ttgctctatc gcaaaattcg
     1621 attgttagcc tatgcaatgc cctggtggga ctggctggca ttgccgactg gcgcgggttc
     1681 gactcccgtt attagccaat taaggtcaac cccacacagt tttgagattg ttggcaggtg
     1741 ggccttgcta atacgtccag ttttcagcca gccggtaacc gcctggtctg tgacaccaca
     1801 ggcatgagcg atagcggttt ggctcggacc tcccttaagg gtgagtttta gcctttcggc
     1861 cattgccggg tcgcttttct tggtcggttt ggagttaagc atcgcttaat tctcccatgc
     1921 cgcgcttgcg gtcacaataa agctatgctt taatccgaaa ctaaagcata gctttggttt
     1981 ggacatgatc gatacagcca taaaacactt tgaatcccga ggtgagttct gccgcgcagt
     2041 cggcatgaca cctcaattcc tatcccagat agaggccaaa aaaaggcccc taccccctaa
     2101 atttgctctg aaaattgagc aagttaccaa tggcgcaatt aaaagagagc agttgcgccc
     2161 agatatttac ccaccaagtg acaactaaac ccaaccaaaa aggaaatagc catgacccat
     2221 tcaaatcaag cactgataaa aaaacgaacc attaaaacca tggtgaatga atttcagttt
     2281 gccgcatgga agcgttttgc aaagaccctt gagttagagc cgcaagagtg gcttaggaat
     2341 ctggtcatgg aaatcgttct cgcggctaac gctgctgaag atgcgaaatt gaacggtagc
     2401 aatgttcgtc agttcccagc tcgcagggct tcggacaggg taattaaata cgctttggca
     2461 aattaatgcg cgaccctctt ttaggcccta actggggcct ttttttgggt tttttttcag
     2521 gtacctcaaa aaggcttggt ggtcataatg gaaagcatca ttagaaaaat taaatcctgc
     2581 ctagcgctgg ccgggagtga caacccacac gaagctgccg cggcgatgcg ccaagccaaa
     2641 aaacttatgg cgcagcatgg tattggtgag tcgatgcttg atgtgtcgcg aaccgtttta
     2701 aatacgcgat atgcacgacc gcccgcctgg tttggctctc ttggttttgc agttgggcag
     2761 gcgtttgggt gttccgtttt tgctgctcgc agggtggtta cttttgttgg gccaactggt
     2821 gcggccgata ttgcagcgta ttgttttgaa gttgttctgc ggtcgctgga ggcatctaaa
     2881 agcgccttca ttaaacgcac cgcctacctt tgcagcgcgc cagttaaaaa gcagcgcgcc
     2941 acttcttatt gcgaaggctt tgttatgggc gctcaacggg cagtagattc ttttgcgcca
     3001 gcaattgaca aggcagccaa gcaggcccac cgggattatt tgaccgaagt tactggcagc
     3061 ggaattttca gcagcaaaaa aaagaaaacc gtcgaaataa acccctctct aatttccggt
     3121 tttcaggatg gcgagcaagt tcaaatacgc gcgccgattc atcaacagca acagcagatc
     3181 gaattcctgg ggggccgccc atgaaatatt tcctgttttt aaaattcctg attattgagc
     3241 tcctgtctga gtttgatgag gtgctggccg ctcgggcgca atttaaggcg gtggagcaga
     3301 tcactcaaag cgggtggaat gattagcgaa tgaatctact tgtggcccca ctagaggcgc
     3361 ttacggacaa gctgctaacg gataccgaga gacgagtcct cttggcgctt ttttcgtttc
     3421 gcagcaagtc aacgaatgta gtttggccat ctactgcaag tatcgctgag cgggccaata
     3481 ttggcgacat accgagggtg tcgaaaataa catcaagcct ggctgacaag ggctggctaa
     3541 cgaaaaagaa aaaggggttt actgggtgca atgagtaccg cttaaccttc ccagaaaggc
     3601 ttaataccaa tttggcagaa tttaccaact tggaagaatt aaccaatatg gacgaggata
     3661 ccaataccaa tttggcagaa tttaccaagt ccaatttggc aaattttgcc aagtgcaatg
     3721 aacaatccat agaacaatcc attgaacaaa ccatatctct tgaaaattcc accccagaga
     3781 aaaaatcaaa accggaatat ccagcctggt ttgaaaacct gctgagcgag ttcccccgga
     3841 gggcggggag caacgacaag cgcaaggcgt acatcgcagc caacgctcgc accaaggagg
     3901 gcgccaccca tgacgaactg cttgaggctg ttcgccgcta tcgccggtac gtaaccgcaa
     3961 acggcaacct aaacacccaa tacgttttgc agtgggcaac attttttggc cctggtgggc
     4021 acatcaaaaa tccgtggacg attcccttgt tcaccgctca accaagagcg gcaccgggcg
     4081 ctggcacact tggatttaca ccggaggatt accagcgcgg aattaaccca gacgggagtt
     4141 tttgagaatg accgatgcag tcgaaacaga tattcgagat tacgttcgag agctcgtccg
     4201 cgcagccagg cgccgagcgg gatttgagtt tttggggcaa aaagaatccg agtgtgagcg
     4261 ccacgggaaa tttattcatg atgaatttat cgacaaagac ggattggtaa cggccggggt
     4321 ttgtcctgaa tgccacgccg aaaacgagaa gcaacgcgag cgcgaagagt cgatcaggat
     4381 gcaggcggaa aatcgcgcta gagcgatagc gagcaaattt gaatccgctt gcatacctca
     4441 gcgtttcaaa gatcgcaccc tgtcaaatta cgaaattcac tgtgacaagc aggcggcagt
     4501 tttggagttt tgcaaaacct acgccgaaag atttaacgag gttctgcggc ttggcagttc
     4561 gataatttta tctggcacag tgggcacggg aaaaacgcat ttgagcatcg gaattgccaa
     4621 tgaaattatt tccaatggtt acgccgcgtt attttctacg gcggctggaa tagttcgccg
     4681 cgtacgctct agctggggga aaaacgacga ggatgagtcc gccgcaatga atatttttat
     4741 catgcctgat ttgctcatcg ttgacgaagt tggggttcaa tctggcagtg ataacgagca
     4801 ccaaatcctt tttgagattc tgaatgcccg ctatgaggcc tgcaagccga caattttgct
     4861 aaccaattta ccgctgcagg atcagactga aaatggctcc attgttcgca aggggcttaa
     4921 atcatttatc ggtgagcgat tgcttgatcg tatgcgcgaa ggcggaggaa gggcgttcac
     4981 gcttgactgg aaatccggac gaattggccg ttaatcaatt tttgggggca aacatggcaa
     5041 cagaaaaaac agtaaaaaca gtaaaaacag tgaaagaaat cgtaaaccgc attatcgctg
     5101 ccaatgaaga gtcgcctatt gctgttgaat tagttgacgg caatttgcgc agctattttg
     5161 caaagactgt cccggcgcct aaatcaaaaa cggaaaagcc tggcgagaat gggcgcattc
     5221 agggcgtcgt tatgctcatg cctcgtcgcg atgaagagaa ttttcttggc gttttccatg
     5281 gcgctattgg cgcggccaat tttaaaaaaa tgtgctttgg tttgggcttg accaatggct
     5341 gaggaattta ttgtcaacag tcgcgcaacg cttgccgctg cacatgcacg gttgattgat
     5401 aatttcgata agcataagca caccgtttat aaggtggtta attgcgaaga gggaacgctg
     5461 ccaatgaact cactggtgca tgtttggcta actgaatttg cggctgatct ggcgcagtgc
     5521 catccaaagc aggttaccaa gggaatgcta gagggaatta agcgcagcgc aaaaggttgg
     5581 ttttatcacg aaacgcaaga gccgtggatg gtgcacaaag tttattgccc actcaccaag
     5641 cgcagcaaaa cggattacac gagcatgaag gattggaaaa aaggcgagaa attaatgttc
     5701 atgaattggc tgcagatgtt tgcagcgcag cgcggaacaa ttttggaatc aaaaggcgag
     5761 tacttagacg ccgttaaaaa acaaaacgct tagggggttt tatgtggttt aagaatttac
     5821 gaatttacag tattgagttt gatcttgatg cagaggcact agctgccgcc ctcgatgcac
     5881 acaagtttgt gccttgcggg aatctggacg tttcgcgcta tgggtttgtg ccgcctctgg
     5941 gtgatcacgg cgaaagcctg gttcacgtca ccaatggctg catcatgatt tgcgcaaagc
     6001 atcaggagaa aatattgccc ggtgccgtga ttaaatccgc gcttgaggaa aaagttaagg
     6061 agattaacgg gcgcggcttc gcggttagcc gcaaagagcg ccaggattta aaagacgaaa
     6121 ttatattttc gttactcccc cgtgcatttg taaaagaccg cctagatttt gcgtacatcg
     6181 acacaaaaga gcagcggtta atcgttaatg cggcatctgc aaaacgcgct gagcggttga
     6241 ttcatgagtt gcggttggct attggtagtt tgaagtgcac accatttgag tgtgatcgcg
     6301 gcgcagaatc gctattaacc gagtgggtgc gccagggtta tgccgttgag ccgttttatc
     6361 ttggcgaatc gtgcgagctc gtcggaccta aagacgggag aagtattacg gctaaaaagc
     6421 atgacatgag ttcagaggaa attggagtcc atttgaactt tgggttgcgc gtagaaaaag
     6481 cgtctctatt ttggggtgag gatttatttt ttacgcttga tgccggtctt ggaattaaaa
     6541 aacttgatta ttcagatgcc gtcagtgagc aagtggaata ccgcaaacca gaaagcgcgg
     6601 cggaagaatt tgacgcgacg ttttttatta tgtccgagac actgcgccgg tttgttaaag
     6661 atttggctgg tgcgctgggt ggcgaagttg gcagcccgca agaaggcgaa taatgaaaaa
     6721 ttccgaccgc ttgcaccttt caaaagtcgc cgctctgggc tgtatcgctt gtatcaacat
     6781 gggccatcca gacacccctg cggaaattca tcacccaagg gcaggggctg gcatgggcca
     6841 gaaatcaccg cataaaaacg ccatcccgct ttgccaccgg catcaccgaa cgggcgggcc
     6901 tgggattgct attcatgcag ggcaaaaaac ctgggaggcg attcatggca cggaattgga
     6961 gctgctgggg caggtgaggg agctgctggg ggttacatgc taaaaatcct cgtgccattc
     7021 gttgggcctt ccaccaattc aatttatgcc ggtgtgcact gggataaacg gcgcaaccaa
     7081 aaagagcagg caatgattgc cttgtcagct ctggggcgaa ttaatccagt ttcaaagcca
     7141 gtgaatattg aggtgacacc ctatctcgga aagggtaagc gcgggtatga cgtgagcaat
     7201 tacagctaca catacaagct aattgaggat gcgatggtta aaaagggaat cctgcaggac
     7261 gactcaccgc aatttgtgcg cagcgtgaaa tttaacgcgc cagtgcggca cagcgaaaaa
     7321 cactctgcaa ctctaataac tatcgaggag attaattgat gcgattcctg tgcgcccgcc
     7381 aagcctggca cgatgcgttt atgactgatc tggctgcccc cgattttgct acccaggccg
     7441 ctaatgtggg agtgcagaaa acagtgcgcg gggctaacaa ttcgattgtg gaccactgcg
     7501 agcgtgggta catcatcgct gccgtccacc gactgcggga ggttgattac attgcttact
     7561 gctgggggat gatcgcttat gccccccagg gcaccgccag ctttgcagag ttcgccgcaa
     7621 tgcacggttt tatgcgggac gctttttt
//
LOCUS       DAOQGS010000148         7645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4605_length_7645_cov_4.316206, whole genome shotgun sequence.
ACCESSION   DAOQGS010000148 DAOQGS010000000
VERSION     DAOQGS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4605_length_7645_cov_4.316206"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="asd"
                     /locus_tag="PKZ52_07340"
     CDS             complement(<1..924)
                     /gene="asd"
                     /locus_tag="PKZ52_07340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNG59625.1"
                     /translation="MLRVGFVGWRGMVGSVLMGRMLEENDFALIEPVFFTTSNVGGKG
                     PAIGKEIAPLKDANSIDELKTLDAIISCQGGDYTNDVFPKLRAAGWNGYWIDAASSLR
                     MKDDAIIILDPVNKDVIEQGLAKGVKNYIGGNCTVSLMLMGLGGLFKAGLVEWVSSMT
                     YQAASGAGAQNMRELISQMGAIHASVADKLADPASAILDIDRQVAATQRSSDFPTANF
                     GAPLAGSLLPYIDKQWETGQSKEEWKGMAETNKILGPTSAIPIDGLCVRIGAMRCHSQ
                     AITLKLNKNVPMADIEQLIVQANDWVTLVPNT"
     gene            complement(979..2070)
                     /gene="leuB"
                     /locus_tag="PKZ52_07345"
     CDS             complement(979..2070)
                     /gene="leuB"
                     /locus_tag="PKZ52_07345"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNG59626.1"
                     /translation="MSKKIIILPGDGIGPEIITEALKVLKKADELFDLQLSYEEDHIG
                     GSAIDAYGVPLADSTIEKCRTADAILMGSVGGPKWDKLDAAIRPEKGLLKIRYDLGLY
                     ANLRPAILYSQLASASSLKPEIVAGLDILIVRELTGGIYFGQPRGIRVLESGERQGFN
                     TAVYSESEIERIGRTAFEMARKRHKKVCSVDKANVLEATVLWREVMDRLSADYPDVEL
                     SHMYVDNAAMQLVRAPKQFDVIVTGNMFGDILSDTAAMLTGSIGMLPSASLNEKASGL
                     YEPVHGSAPDIAGQGIANPLATILSASMMLRYSLGRSDAADAIEAAVGKVLDQGLRTG
                     DIYSAANPEGTRKVGTREMGEAVVAALTR"
     gene            complement(2307..2639)
                     /locus_tag="PKZ52_07350"
     CDS             complement(2307..2639)
                     /locus_tag="PKZ52_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59627.1"
                     /translation="MRGKIFFIVLILCSIDVFGDDWNAPTTPISQLYVYPNYVVVVQE
                     GNYTAPMCTPGFAWSFLWADFDAATQQRIYSALLAAKLARTPIKPIFSSSSCGPEGKP
                     KFNGQFVL"
     gene            complement(2650..3291)
                     /gene="leuD"
                     /locus_tag="PKZ52_07355"
     CDS             complement(2650..3291)
                     /gene="leuD"
                     /locus_tag="PKZ52_07355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNG59628.1"
                     /translation="MKAFTQLNGLVGPMDRANVDTDLIIPKQFLKSIKRSGFGPNAFD
                     ELRYLDKGEPGQDNSNRPINPEFPLNFPRYQGASILLARENFGCGSSREHAPWALEEY
                     GFRCIIAPSFADIFYNNCFKNGMLPLVLSEAVVAKLFAELYATEGYSLTVDLPSQQVI
                     TPGGETFGFEVDAFRKHCLLNGFDDIGLTLQDAGLIRDYETRRKVEVPWLFNI"
     gene            complement(3294..4712)
                     /gene="leuC"
                     /locus_tag="PKZ52_07360"
     CDS             complement(3294..4712)
                     /gene="leuC"
                     /locus_tag="PKZ52_07360"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNG59629.1"
                     /translation="MAGKTLYDKLWDAHVVSQRDDGSALIYIDRHIVHEVTSPQAFEG
                     LRLAGRKPWRLDSILATPDHNVPTTTKERSSGVAGIEDPISRIQVQTLDENCDELGIL
                     EFKINDQRQGIVHVVGPESGACLPGMTIVCGDSHTSTNGALGALSIGIGTSEVEHVMA
                     TQCLVAKKMRNMLVRVDGQLAPGVTPKDVVLAIIGEIGTAGGTGYAIEFGGEVFRSMS
                     MEGRLTVCNMAIEAGARAGMVAVDDITIDYVKGRPFAPKAEHWEQAVANWRQLYSDSD
                     AVFDRVVLLDGGAIEPQVSWGNSPEMVLPVGGRVPDPALEQDPVKKAGIERALEYMGL
                     EAGQAITDIKVDRVFIGSCTNSRIEDIRAAAAVVRGKTKAASVKEAIVVPGSGLVKQQ
                     AEAEGLHQVFIAAGIEWREPGCSMCLAMNADRLEPGEHCASTSNRNFEGRQGYGGRTH
                     LVSPAMAAAAAIAGHFVDVREF"
     gene            4821..5687
                     /locus_tag="PKZ52_07365"
     CDS             4821..5687
                     /locus_tag="PKZ52_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG59630.1"
                     /translation="MDTQNLKAFLAVAAQGSFSQAASLLHLTQPAVSKRVAALEDEVG
                     HALFHRVNRRVELTQAGVLLQQRAMGILQALEEARQALADLDGAVSGELKVATSHHVG
                     LHKLPPVLHEFVHRYPRVNLQFEFLDSEIAYQRVAKGDCELAVVTLGPTPHPVLTERT
                     LWPDPLVFVASKGLFNSAADTIPLEHLSRLPAILPDVSTYTGRLVKACFDEHGLPLNI
                     TMATNYLETIKMLVSVGLGWSLLPQTLLDDQLMVLDVPLVISRDLGVITHPKRALTNA
                     ARAFLDLLGTAG"
     gene            5893..6546
                     /locus_tag="PKZ52_07370"
     CDS             5893..6546
                     /locus_tag="PKZ52_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59631.1"
                     /translation="MPFPLVRPIRNLATLFTLATSLALTSTTHAQDSANHSAVPESAG
                     WEFGLSAGQAQLDKDAAAKAYIGDSADLFTLNVQYYFANSPWMINAGGGSLSYDDQLG
                     FSQVVREEYSGDTYSKDSSASGTILFIEAGANYPLNAALFLQGRLGLSSVFGSSRSIE
                     NCKNCWEEDFDINGGAYGSFTFGGTIANKVELALQYQYYLSGDLASAVSLNIGSHFF"
     gene            6728..7528
                     /locus_tag="PKZ52_07375"
     CDS             6728..7528
                     /locus_tag="PKZ52_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04219 family outer membrane beta-barrel
                     protein"
                     /protein_id="HNG59632.1"
                     /translation="MLAKHMLLVPLLATTAFTLAHNAHADTLGLRAGVEYWNADLSGE
                     AQSQTARIKKTGKPVKFDDWQFYGETYNSVWVNFEHPIPFVPNIRMTYTNIENAKSRI
                     VKYVVGPTFFNTRDSVALTFDNIDFTFYYEVLDNWINLDAGLTARSLNGDFEALSTSI
                     SSLQLSQPSQDRTKLNEVVPMLYLNGRIDIPFLDGLYVQSVLNGATLKGNTLYDVDFR
                     VGYDLDLIALDFGVYGGYRRMALKSTDLGNLYADAYMDGLIAGLEVHF"
BASE COUNT         1866 a         2115 c         1823 g         1841 t
ORIGIN      
        1 ggtattggga accagggtta cccaatcgtt ggcctggaca attaactgct ctatatccgc
       61 catggggaca tttttattca gcttcaaagt gatcgcctgg ctgtggcagc gcatggcacc
      121 gatgcgcaca cacaggccat caatggggat tgcgctggtg gggccgagga ttttgttggt
      181 ctccgccatg cccttccact cttctttgct ctggccggtt tcccattgtt tatcgatata
      241 gggcagcagg ctgcccgcca ggggcgcacc gaagttagcg gtggggaagt ctgagctgcg
      301 ctgggtggcg gccacttggc gatcaatatc caaaatagca ctggcaggat cggcgagctt
      361 atcggccacg cttgcgtgaa tggcgcccat ttggctgatc agttcgcgca tgttttgcgc
      421 accggcgccg gaggcggcct gataagtcat agagctgacc cactccacca aaccggcttt
      481 aaataagcca cccaaaccca tcagcatcag gcttacggtg cagttgccgc cgatataatt
      541 ttttacgccc ttagccaagc cctgctcaat aacatcttta ttcactgggt cgagaataat
      601 aatggcgtcg tctttcatcc gcagagagct ggccgcatca atccaataac cattccagcc
      661 tgcggcgcgc aatttaggga atacgtcgtt ggtgtagtcg ccgccctggc agctaattat
      721 tgcatcgagt gtttttaatt catcaatgct attggcatct tttaagggcg cgatttcttt
      781 acctatagcg gggcctttgc cgccaacatt ggaggtggta aaaaataccg gctcaattag
      841 ggcaaaatcg ttttcttcta acatacggcc cattaatact gagcctacca ttccccgcca
      901 accaacaaat cctactcgta acataaaatc acctgtttaa actgtttaat aatggctatg
      961 aatttaattg cggcgaattt agcgcgttaa agcggccacc accgcttcgc ccatttcgcg
     1021 cgttccgacc ttgcgggtgc cttctggatt ggctgccgaa taaatatcgc cagtgcgcaa
     1081 gccctgatcc aataccttac ctaccgccgc ttcaatggca tcggcggcat cgctgcggcc
     1141 caaggaataa cgcagcatca tagaagcact taaaatagtg gctaatggat tagcaatacc
     1201 ctgcccggcg atatccggcg cggagccgtg cactggctca tacaagccgg acgctttttc
     1261 atttaatgaa gcagagggca acattccgat ggagcctgtg agcattgccg ctgtgtcgga
     1321 gagaatatcg ccaaacatat tgcccgtaac aattacgtca aattgcttgg gcgcgcgcac
     1381 caactgcata gccgcgttat ccacatacat atggcttaat tccacatccg ggtaatccgc
     1441 acttaaacgg tccatcactt cacgccacaa cacggtggct tccaacacat tggctttatc
     1501 caccgagcag acttttttat ggcgcttgcg ggccatttca aacgccgtgc ggccgatgcg
     1561 ctcaatttca ctttcgctgt aaacagccgt attaaaaccc tgccgctcgc cgctttccaa
     1621 tacgcgaatg ccgcgcggtt gcccaaaata aatgccgccg gttaattccc gcacaattaa
     1681 aatatccaag ccggcaacaa tttccggctt taagctggag gcactagcca attgcgaata
     1741 caaaattgcc gggcgcaggt ttgcataaag gcctaaatca tagcggattt ttaacaggcc
     1801 tttttccggc cgaatcgccg catctaattt atcccactta ggcccaccta cagagcccat
     1861 taaaatggca tcggccgtgc ggcatttttc gatggtgcta tcggctaatg gcacgccata
     1921 ggcgtcaatg gcgctaccgc ctatatgatc ttcctcgtag cttaattgca gatcaaaaag
     1981 ctcatcggcc ttttttagga cttttaaagc ttcggtgata atttccgggc ctatgccgtc
     2041 accgggaaga ataataattt ttttgctcaa gggggattcc tctttctgtg ccacgtttag
     2101 cggcagtatt tttcagttaa atttcttgct cccacactct tgcgagagaa cacacacaac
     2161 ttgcaacaca tataaattag agttgcatcc gtaccgcgca ttaactactt acccgtaggg
     2221 cgtcaccttg gtgcgccaca ccgaagtctc agacacaccg aaaacggcgc accaaggtgc
     2281 gccctacgtg tagtccgggt tacgcgttaa agaacaaact gtccgttaaa cttcggcttc
     2341 ccttcagggc cacaggaaga gcttgaaaat ataggtttta ttggtgtcct agctagcttt
     2401 gctgctaaca aagcactgta aatcctttgt tgagtagcag catcgaaatc agcccacaaa
     2461 aaactccacg caaaacctgg cgtgcacatt ggcgctgtgt aattaccctc ttgcaccacc
     2521 acgacatagt ttggataaac atataattga cttatcggtg ttgtcggagc attccaatca
     2581 tctccaaata catcgataga gcataaaatt aaaacaataa aaaatatttt tccacgcata
     2641 aatttactcc taaatattaa acaaccaagg cacttcaacc ttacgccgag tttcataatc
     2701 ccgaatcaac cccgcatcct gcaaagtcaa cccaatatca tcaaacccat tcaacaaaca
     2761 atgtttacga aacgcatcca cttcaaaccc aaaggtctca ccgccaggtg taatcacctg
     2821 ttgcgatggc aaatccaccg tcaagctata accttccgtt gcataaagtt ctgcaaataa
     2881 tttagcaaca accgcctcag acaacaccaa aggcaacatg ccatttttaa aacagttgtt
     2941 ataaaaaata tccgcaaaac tcggcgcgat aatgcagcgg aagccgtatt cctctaaggc
     3001 ccagggagcg tgttcgcggc tggagccgca gccgaagttc tcgcgggcaa gcaaaatact
     3061 ggcgccttgg tagcgcggga aattcagcgg gaactcgggg ttgatgggcc ggttggaatt
     3121 atcctggccc ggttcaccct tgtctaaata acgcaattca tcaaaggcat tggggccgaa
     3181 gcctgagcgc ttaatggatt ttaaaaattg tttgggaata attaaatccg tgtccacatt
     3241 ggcgcgatcc atggggccta ccaaaccgtt taattgggta aatgctttca tgattaaaac
     3301 tcccgaacgt caacaaagtg gcctgcaatt gccgcagcgg cggccatggc ggggctgact
     3361 aaatgggtgc ggccgccata accctggcgc ccttcaaaat tgcggttgga ggtggaggcg
     3421 cagtgctcgc caggctctaa gcggtcggca ttcatggcta aacacataga gcaacctggc
     3481 tcccgccact caatgcctgc ggcgataaat acttggtgca ggccttcagc ctccgcctgt
     3541 tgttttacca agccggagcc gggcaccact atggcttctt taaccgaggc ggctttggtt
     3601 ttacccctta ctactgcagc tgcggcgcga atatcttcga tgcgcgaatt agtgcaggag
     3661 ccgataaaca cgcggtctac ttttatgtcg gtaatcgcct ggcccgcctc taaacccata
     3721 tattcaaggg cccgctcgat accggctttt tttaccgggt cctgctccaa agcggggtcg
     3781 ggcactcgcc cgcccacggg caacaccatt tccgggctat tgccccagct cacttgcggt
     3841 tcaatggcgc cgccatccaa tagcactaca cggtcaaaca cggcgtcgct atcggaatac
     3901 aactgccgcc agttggccac cgcttgctcc cagtgttcag ctttaggcgc aaaggggcgg
     3961 ccttttacgt agtcgatggt gatgtcatcc accgccacca tgccggcgcg agcgcccgct
     4021 tcgatggcca tattgcacac ggttaagcgc ccttccatgc tcatggagcg aaatacttcg
     4081 ccaccaaatt caatggcgta gccggtgcca ccggcggtgc caatctcgcc gatgatggcc
     4141 aacactacgt ctttgggggt aacgccgggg gccaactggc cgtccacgcg aaccaacata
     4201 ttgcgcattt ttttcgccac caaacactgg gtggccatca catgctccac ctcgctggtg
     4261 cctataccga tggagagcgc acccaaagcg ccattggtgg aggtgtggga gtccccgcag
     4321 acgatcgtca tgccgggtaa acaggcgccg gattccggcc ccaccacatg cactatgccc
     4381 tggcgctgat cgttaatttt aaattccaaa atccccaact catcgcagtt ttcatccagg
     4441 gtttgcacct gaatgcggga gatagggtct tcgataccgg ccacaccaga ggagcgctct
     4501 tttgtggtgg tgggcacgtt gtgatcgggg gtagccaaaa tgctgtccaa acgccaaggt
     4561 ttgcggcccg ccaaacgcag gccctcaaag gcctgaggag aggtcacttc gtgcactatg
     4621 tgccggtcga tatagatgag ggccgagccg tcatcccgtt gcgacaccac gtgggcgtcc
     4681 cagagtttgt cgtaaagtgt tttgccagcc ataaaaatcc ccttcggttc ttgaccacat
     4741 gggtttattg tcgcaaccta aatcaaataa ctccaattca ttctttttat gttttgaatt
     4801 ccaaacagga atacaaatct gtggacaccc aaaacctcaa agccttttta gccgtggccg
     4861 cccagggcag tttttcccag gcggcgagcc tcttacatct aacccaaccg gcggtaagca
     4921 agcgcgtggc ggccctggag gacgaggtgg gccacgccct atttcaccgc gttaaccgcc
     4981 gggtggaatt aacccaagcg ggagttctgc tacagcagcg cgccatggga attctgcaag
     5041 cgttagagga ggcccgccag gccctggcgg atttagatgg cgcggttagc ggggagttaa
     5101 aagttgccac cagccaccat gtgggcctgc ataaattgcc gccggtgctg catgagtttg
     5161 ttcaccgcta cccgcgggtg aacctgcagt ttgagttttt ggattcggaa attgcctacc
     5221 agcgggtggc caaaggcgat tgtgagctgg ccgtggtgac cctcggcccc acgccacacc
     5281 cggtgctcac cgagcgcacc ctctggccag acccattggt atttgtagcc agtaaaggct
     5341 tgtttaacag cgcagccgat accatccccc ttgagcacct aagccgcctg cccgccattc
     5401 tgccggatgt aagcacctat accggccgcc tggtgaaggc ctgttttgat gaacacggcc
     5461 tgccactgaa tatcaccatg gccaccaatt atttagaaac cataaaaatg ttggtgagcg
     5521 tgggtttagg ctggagcttg ctgccgcaaa ccctgctgga tgaccaactg atggtgctgg
     5581 atgtacccct ggtaatcagc cgtgatctcg gcgtcattac ccaccccaaa cgagcactca
     5641 ccaatgccgc acgggcattt ttagacctgc tgggcactgc tggttaggcc ctggaagacc
     5701 acacaccgca aatgccttga ataaaagcgg cgcaaatttt tgcaaagatc tgcgcaattt
     5761 cagcccttaa agccctgcct tgcgagttca ccggaaattt ctgggttaaa ctcagccgcc
     5821 ctaagctcaa tgacgctttt ggtgtactga tttttaattg tttagatttt gatttttaga
     5881 taaggaaccc ttatgccctt ccccctcgta cggcccatcc gaaacctcgc cacgctgttt
     5941 acactcgcaa ccagcctagc cctaacctcc actacccacg cgcaagactc ggcaaatcat
     6001 tcagcagtac cggagagtgc aggctgggag ttcggtcttt ccgccggtca agcacagctg
     6061 gataaagacg ccgccgccaa ggcctatata ggcgatagcg ccgacctgtt taccctcaat
     6121 gtgcagtatt acttcgccaa ctcaccctgg atgattaacg caggcggcgg ctcattaagt
     6181 tacgacgacc aactaggctt tagccaagtg gtgcgcgaag aatactcggg ggatacctac
     6241 agcaaagatt cctccgcaag cggcactatt ttatttatcg aggcaggcgc caactatccg
     6301 ctcaatgctg cgttattttt gcagggccgc ctcgggctta gcagcgtatt tggctcatcc
     6361 cgcagcatcg aaaactgcaa gaattgttgg gaggaggatt ttgacattaa cggcggtgct
     6421 tatggctcat tcacctttgg cggcaccatc gccaataagg tggaacttgc cctgcaatac
     6481 cagtattacc tgtccggcga cttagcctct gcggtaagcc ttaatatagg ctcgcacttt
     6541 ttttaactgg caaaacctgc gtgtgtatct gcccgaaagc attcacggca aggcatttcc
     6601 ggcttggatt acaacaggtt tcacaaacgc atcgagtgtg cagacaaaaa tgccgccagg
     6661 ccaacagcct cgcttcaata tgcattaaac tgcccaggcc gattaattct aacgttggaa
     6721 gacgaacatg ctcgccaagc acatgctcct tgtacctcta ctggctacca ctgcgttcac
     6781 gctcgcacac aatgcccatg ccgacacctt aggcctgcgg gcgggggtgg aatattggaa
     6841 tgcggattta tcgggtgagg cgcagagcca aaccgcccgc attaaaaaaa ccggcaaacc
     6901 agtcaagttt gatgattggc aattttatgg tgagacgtac aattccgttt gggtgaattt
     6961 cgaacaccct atcccgttcg tgcccaatat tcggatgacc tataccaata tcgaaaatgc
     7021 aaaatcacgt atcgtcaaat acgtggttgg ccccacgttc ttcaacacac gagacagtgt
     7081 tgcattaacg ttcgacaata ttgattttac gttttattac gaagtgctgg acaactggat
     7141 caatttagat gcgggcctta ctgctcgctc cttaaacggt gattttgaag cgctgtcaac
     7201 ctcaattagc tctctacaat tatcacaacc ttcacaagac cgtacaaaat taaatgaagt
     7261 agtgcccatg ctgtatttaa atggccgcat tgatattcca tttttagacg gcctttacgt
     7321 acagtcggta ttaaacggcg ccaccctcaa aggtaatacg ctttatgatg tggattttcg
     7381 cgtggggtac gatttagatt tgatcgccct agattttggc gtgtatggag gctaccgccg
     7441 catggccctt aaatccaccg acctaggtaa cctctatgcc gacgcctaca tggatggttt
     7501 aattgccggg cttgaagtac atttttaatc ataaattaat tcttagtgaa gccgccggga
     7561 gctgcgctca aacaagaaaa tccgcgcatt ttcgtaggtt tagcgcagcc taggcaaaat
     7621 agatcaggac gatctatttt gccgc
//
LOCUS       DAOQGS010000149         7618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4628_length_7618_cov_4.961259, whole genome shotgun sequence.
ACCESSION   DAOQGS010000149 DAOQGS010000000
VERSION     DAOQGS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4628_length_7618_cov_4.961259"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..451)
                     /locus_tag="PKZ52_07380"
     CDS             complement(194..451)
                     /locus_tag="PKZ52_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59633.1"
                     /translation="MSENSLFALFFFPSLAAVLIFSMKYVAAILQAKFRLSQDEAYRE
                     MAAKTAAAQAEMVEGFSAMQNTLVDIQLKLASLEKILKDVE"
     gene            complement(460..792)
                     /locus_tag="PKZ52_07385"
     CDS             complement(460..792)
                     /locus_tag="PKZ52_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNG59634.1"
                     /translation="MTDENTYPSGDQLLAMLAALANPHRLRIMGALHLDGRNYVSQLA
                     REIGISRPLLQLHLQKLADAGLVTSKLELSEDGKALNYYEVARFAVQLTPALVAEAAR
                     SLTLNPDN"
     gene            complement(926..1708)
                     /locus_tag="PKZ52_07390"
     CDS             complement(926..1708)
                     /locus_tag="PKZ52_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNG59635.1"
                     /translation="MSQKLLNLSSRLWFVTAAAGQLAFVLFIVTYYGSRTWRGDFAAW
                     NNKPVITGFVPGDSLGNLLFALHVLLGGVITLAGLLQLVPRIRQAAPGLHRWNGRLFL
                     ALAVFLALDGVWKILVRGSTLSTASTLSGLVGAALILLCCALAFYFARRGQVVKHRRW
                     ALRAFLVVSGVWFFRVGIMAWVILNQGPRGMNATLSGPADVALAIGSYCVPLAGLQMY
                     LWAQSSAAAGVKNLVSLLIVLLTGLMAVGIVGTVSMMWWPYL"
     gene            1840..2853
                     /locus_tag="PKZ52_07395"
     CDS             1840..2853
                     /locus_tag="PKZ52_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG59636.1"
                     /translation="MNVDSFKHIPRLSLRLPAQLLQPLNLYGVLFALAILSFTLKQTV
                     LESIPALAWLLSIASCVTCGLAWLLSRALFKPPGPRPLWPPLLVAGQFALTAALILLE
                     TWLPNLHTSNLIGMLNKLVTLFSSTVLLLPLVEATEGLRAVGDKKEQGFRLIYAAGYS
                     LLLLTSLAASLPALAPWQGEIKTYLAIVALLATARAMHYRLGHPLLEPAQNRQRKKIQ
                     PLEIDPTIAHKVKTQLEQHQIFLDAEIKVAGLAQLIGEQEYKVTQCITGDLQFRNFNH
                     MVNTYRIAHAQTLLANPACADSSILTIAMESGFNSLGPFNRAFKALVGKTPSEYRSEV
                     AAN"
     gene            complement(3536..3862)
                     /locus_tag="PKZ52_07400"
     CDS             complement(3536..3862)
                     /locus_tag="PKZ52_07400"
                     /inference="COORDINATES: protein motif:HMM:NF026932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm50 family immunity protein"
                     /protein_id="HNG59637.1"
                     /translation="MTDYQAEVVEFFGYWPAFCDGHVVSYENTGNSIKLRIDYIDSDK
                     SLRALVEISFQEVSDAYLSDYVPESVVSNLSILRGSQHWVSLEPCYGLGGSFKCKSVQ
                     AVIVNS"
     gene            complement(3906..4289)
                     /locus_tag="PKZ52_07405"
     CDS             complement(3906..4289)
                     /locus_tag="PKZ52_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59638.1"
                     /translation="MAHENSNIIRELTAKERVLVEWLARNPTVETDIPLSELDELSVV
                     SECRCGCASIDFAINGSPVDQRGGITVIADYLYHSEQKFQMGCFLFMSNGHIAGLEVW
                     SVDGAETPSRLPLPVELYGYENAGR"
     gene            complement(4422..5366)
                     /locus_tag="PKZ52_07410"
     CDS             complement(4422..5366)
                     /locus_tag="PKZ52_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59639.1"
                     /translation="MPFIGTKSDEVTLKLFQWTLFVDMLGYRDANGSICSDEDAKDFV
                     EFMEVNRNILDFSNRTEVKERYEKDDFDLYKYYDIDSCFVSDSIIITYKPKEVGELIN
                     DELRFLHSANALFIICMRLQTVIFNCFTEKGIFLRGGISSKYAYIKYNFAVGEGVIEA
                     YLAESEVAKNPRIVLHPNIRENNRLIEKIELLSKLMYGGKSLIQSDPKDGNLFLDYIG
                     YAISSSSLKSAAVARAALINPIGLIAQKAVTKKFIQRHSEALKRKIDEITENLERVET
                     GSKEQEKIERVLSKFIWLKEYHNRSLAGEKELGSYLIE"
     gene            complement(6122..6535)
                     /locus_tag="PKZ52_07415"
     CDS             complement(6122..6535)
                     /locus_tag="PKZ52_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59640.1"
                     /translation="MNNKRNLLAIIAFLLIFLCVSFYVGCALNSAPAFAVCVATLLPL
                     VAIYCVGVLDYKVGGSQLSLEPRVHNLEEQTAELQKVSTALLKALYLVADGAPRMGGV
                     PNKHNDLLNDYLKDIAHLVDSDVRNKVEADIRSID"
     gene            complement(6666..6959)
                     /locus_tag="PKZ52_07420"
     CDS             complement(6666..6959)
                     /locus_tag="PKZ52_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59641.1"
                     /translation="MLFDKIIPGLILAAITGTVWTFFFKPDLFVSVAMYEALLLTIVV
                     FGVFMICVGAEIGRDHKIFKGKNPMHSFAMIFLYWTGSVLCLGFLSYLVARTQ"
     gene            7079..7309
                     /locus_tag="PKZ52_07425"
     CDS             7079..7309
                     /locus_tag="PKZ52_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59642.1"
                     /translation="MIVERLPFESEQGEATPKSFLSAALHELEENNLLGNVSVYLICA
                     MEALDRGVAYSESFICKDEIERCKEVLNRSKR"
BASE COUNT         2007 a         2019 c         1679 g         1913 t
ORIGIN      
        1 ctcacggcgg tttgcaccgc cggggcggca tccatgccgc cattattaac ccggcggcta
       61 gcgccctttg tgcggcaccc ttgccgcccc attaaatctg caaaaaagtt aacccggcat
      121 acaaagccgc caggttaaat ctgccgcaca atttttatcg tgcgttacat aattcgctta
      181 aaaatctgcc gcactactca acgtctttta atattttttc caggctggcc agttttaact
      241 gaatatccac cagtgtattt tgcatagcag agaatccctc aaccatttca gcctgcgcgg
      301 cagctgtttt cgcggccatt tcgcgataag cctcatcctg ggaaagacga aattttgcct
      361 gcagaatagc ggcgacatat ttcatagaaa aaatcagcac agccgccagc gacgggaaaa
      421 aaaacaatgc aaaaagtgaa ttctcagaca taagcgcctc taattatcgg gattaagtgt
      481 tagggagcgg gcggcttcgg ccactaaggc cggcgtcagc tggaccgcaa agcgcgccac
      541 ctcgtaataa ttcagcgcct taccgtcctc agacaattcc agcttgctcg tcaccaggcc
      601 cgcatccgca agcttctgca aatgcagctg cagcagtggc cggctaatgc cgatctcacg
      661 ggccagctgg ctcacataat tgcggccgtc caggtgcagc gcccccataa ttctgagccg
      721 gtgcggattg gcgagggccg ccagcatcgc cagcaactga tctccacttg gatatgtgtt
      781 ttcgtctgtc atgtgcaaac aatatcttgc aggtgaaaga aagtcaaacc atccatggca
      841 attttatttg ctcagggcgc gagccgtttc tcaggcgccc ccgtgcatca aggcccaccc
      901 ccttattagc ccagtattta cggcactaaa gatacggcca ccacatcatg gacacagtgc
      961 caacaatccc taccgccatc agccccgtca gcagcacaat aagcaggctc accaggtttt
     1021 taacccccgc cgctgcactg ctttgcgccc acaaatacat ctgcaaaccc gccagcggca
     1081 cacaataact gccgatggct agggccacat ccgcaggccc agagagggtg gcattcatgc
     1141 cgcgcggccc ctggttgagg atgacccagg ccataatacc cacgcggaaa aaccacaccc
     1201 cactcaccac taaaaacgcc cgcagggccc agcgcctgtg cttaaccacc tggccccggc
     1261 gagcaaaata aaaagccagg gcgcaacaca ggaggattaa cgccgccccc actaaaccgc
     1321 tcagggttga agcggtggac agagtcgagc cccgcaccag aattttccac accccatcca
     1381 aggccaggaa aaccgccaaa gccaaaaata accgcccgtt ccagcggtgt aatccagggg
     1441 cggcctggcg aatgcggggc accagctgca gcaggcccgc cagggtaatc acgccgccca
     1501 gcaacacatg cagggcaaac aacagattgc ccaggctgtc gccggggaca aagcccgtaa
     1561 tcaccggttt gttattccag gcggcaaaat caccgcgcca ggtgcggctg ccgtagtagg
     1621 tgacaataaa cagcacaaag gccagctgcc cggcggcggc ggtaacaaac cacaaccggc
     1681 tgctaaggtt taagagtttt tgcgacataa aagcttcctg taggattaga gccttttatg
     1741 ttcaagccgg ggcttagctg tgtccttccg atttggaaat cggccaggtc attcagcgca
     1801 aaaccccgct ttcagcccct caaacaaggc ctgccacctg tgaatgtcga ctcctttaaa
     1861 cacatccctc ggttaagcct gcggctgccc gcgcaattgc tgcagccgct caacctctac
     1921 ggcgtgttat tcgccctagc gatcctgagt tttaccctca agcagactgt gctcgaatcc
     1981 atccctgcat tggcctggct gttgtccatc gccagttgcg tgacctgtgg tttggcctgg
     2041 ctgctcagcc gcgcactatt caaaccgccc ggcccccgcc ccctctggcc gcccctactc
     2101 gttgcgggcc aatttgccct cacagccgcg cttatacttc tggaaacctg gctgcccaat
     2161 ctgcacacca gcaacctcat cggcatgctc aacaaactcg tcacgctatt tagctcaacc
     2221 gtattattgc tgcccctggt ggaagccacc gaagggctgc gcgccgtcgg tgataaaaag
     2281 gaacaaggct ttcggctgat atacgcggcg ggctacagcc tattactgct cacctcccta
     2341 gccgcctcac tacccgcgct tgccccctgg caaggcgaaa ttaaaaccta cctggcaatt
     2401 gttgccctgc ttgctaccgc ccgcgccatg cactatcgcc tcggccaccc gctgctggaa
     2461 cctgcgcaaa acagacagcg caaaaaaatc caaccattgg aaatcgaccc gacaattgcc
     2521 cacaaggtaa aaacacagct ggagcaacac cagatttttt tagatgcgga aattaaagtg
     2581 gcaggccttg cccaattaat tggcgagcag gaatataaag tcacccaatg tattaccggc
     2641 gatttgcaat tccgcaattt taatcacatg gtcaacacct accgcatcgc ccacgcccaa
     2701 acattactgg ccaaccctgc ctgcgcagac agttctattt taaccattgc catggagagc
     2761 gggtttaact cgctagggcc gtttaaccgc gcgtttaagg cgttagttgg caaaacaccg
     2821 agtgagtacc gcagtgaggt tgcggctaat tagcctgcgg gcggcaatga gcaacctgct
     2881 tggcgatata attaatatgg agtgtaaata ggcttaatga acgcgatgcg cgcaactggg
     2941 caccgcgcta ttaaaaattc gatgtcaaaa atcgtgattt gtgcagagcg tgttgcaagc
     3001 taggcgaccc aaaaagcaag ggattttcct tggcggaaaa atcaatccgc gcggctcaag
     3061 cgccctacac agctattaaa aaactccgta gggcgtcgcc ttggcgcgcc acaccgcagt
     3121 tcttgacact acgaaatggc gcacttaagt gcgccctacc tgtgcgggtt acgcgttaca
     3181 tttgcgggtt acgcgtttca atgtatttct tatttcgtat taagcgtagg gtgggcaagc
     3241 ctgcttgccc accatgtgag accagaaaat attcccgaat ttatcgacac agggtgacgc
     3301 ttcgagatgc gtgggcaaca agttgcccac cctgcctgct gttacaaccg aagtaatgtt
     3361 tcagtcatag cccgcaagga cgctcaacct tgagggggga gtccaggaag caacccaaca
     3421 cgcaaggtgg attcggagcg aagcgacaat ccgccatttc tcagccgcga tatcgtcgtt
     3481 caaaataata attctctgcc cttacaccaa tggtgggttg cttcgcgaac ccaccttacg
     3541 agtttacgat aaccgcttgt acactcttac atttaaagct accacctaaa ccatagcatg
     3601 gttcaagact tacccaatgt tgcgaacctc tgaggatgct caaattgctc accacacttt
     3661 cgggtacgta atcagataga tacgcatccg atacttcttg aaagctaatt tcgaccagcg
     3721 ctctgaggga tttgtccgaa tctatgtagt cgattctcag cttgatcgaa ttaccagtat
     3781 tttcatatga aacgacatgc ccatcgcaga acgcaggcca ataaccgaaa aattcgacaa
     3841 cttcagcttg gtagtcagtc atctagaact cacataaggc ccagctttcg ccgtggtact
     3901 gattgttagc gccctgcatt ttcatagccg tatagctcaa ccggaagggg taaacgacta
     3961 ggagtttccg ctccgtccac tgaccagacc tctaagcctg caatatggcc gttagacata
     4021 aataggaagc aacccatctg gaatttttgc tcggagtggt atagataatc tgcgataacc
     4081 gttatgccac cacgttggtc aaccgggctt ccattaattg caaaatcgat acttgcacaa
     4141 ccacaacggc attctgacac cacagataat tcatcgagct ccgaaagcgg aatgtctgtt
     4201 tcgactgttg gattacgggc tagccattct acgagcactc tttctttggc ggtgagctcg
     4261 cgaattatgt ttgaattttc atgcgccatt gtgattgctc tctgtagcgc taacgctgtt
     4321 gtaaccggac caaaatgcag agcggttttg tattataagc gcagcgaaca caaaactgcg
     4381 cagtaatttg gttccggttt acaaccttgt taggcatgta gctattcaat aaggtagctt
     4441 ccaagttctt tttcccccgc tagacttctg ttgtgatatt cctttagcca gatgaattta
     4501 ctcaggactc tttctatttt ttcctgctct ttcgatccgg tttcaactcg ctctaaattt
     4561 tctgttattt catctatctt tcttttgagg gcttcggagt gacgctgaat gaattttttc
     4621 gttaccgctt tttgcgcaat aagcccgatg gggttgataa gcgctgcgcg tgcgacggct
     4681 gcgcttttta gactcgatga ggatatggca tatccgatat agtctaaaaa taggttacca
     4741 tccttaggat cactttgaat caaggatttt ccgccgtaca ttagctttga taagagctca
     4801 attttttcga tgagtctatt gttttctcta atgttgggat gcaggacaat ccttgggttt
     4861 ttcgcaacct cgctttctgc cagatatgcc tcaattactc cctcacctac ggcgaaatta
     4921 tatttaatgt atgcgtactt agaggagatt ccgcctctta gaaaaatacc cttctctgta
     4981 aagcagttga aaattactgt ctgaagcctc atgcatataa tgaataatgc atttgccgag
     5041 tgcaaaaatc taagctcatc gttgatcaat tcgccaactt cttttggttt gtatgtaatt
     5101 atgattgagt cggaaacaaa gcagctgtca atatcatagt atttgtatag atcaaaatcg
     5161 tctttttcgt atcgttcttt gacttctgtg cggtttgaga aatccagtat gtttctattg
     5221 acctccatga actcaacaaa atctttagcg tcttcatcag agcatataga gccatttgca
     5281 tccctgtagc caagcatgtc tacgaataaa gtccattgaa atagcttcaa ggtcacctcg
     5341 tcactttttg tgcctataaa cggcactcgc cgtctatcac agtttctctc gccgtctatc
     5401 acaccccatc gaaacgtcac ttaaagaaca attacgctcc gcgtcaacaa gttgcagcct
     5461 attcagcaaa aagtactgcg catttaattt tagataacac ggcgctgcgc cgtattatca
     5521 atgatatgac tgcgccgttt atcaccttcg cagctagcca tcactaacac cccataaccc
     5581 accctatgag gttttaggcg gaacgcgcga aaaatagggt ggcgtttagc gccttatact
     5641 ttaaataaaa cggacaaaca agaactgagc agataagcag taattgtttt cgacttgcca
     5701 agccgccggg gttactgaca ctgcccgcat attcaattgc agtggacggg tttcaacaaa
     5761 actcagtacc cccaacccta taccattaag ttaaataatt ttggcgcctg cacgccctaa
     5821 aattcccctc ctgcagagct ggcaggtgtg cctgccgggg tggtggcggg acttgtgttt
     5881 tccagggtac gaggaagttt tttacccata gaggctgcaa acaaaaaaac agcgcagcga
     5941 tctacccctc cccctgcggg gcccaatgtc attaagtgaa ggttttcgca ttattttcca
     6001 cagcattctc tctcatccag aaaccccacc ccaaccctcc ccttaaaaag ggaattatat
     6061 acttggctac cttagaaaac ttcaatttcg gtgaaagctg tcgtgagagc aaattaccgg
     6121 cctaatcaat agagcgaata tccgcttcta ccttatttct tacatcagaa tcaaccaagt
     6181 gagctatgtc tttcaggtag tcgttcagta agtcgttgtg cttattcggc actccgccca
     6241 ttcttggggc gccatctgca actaaatata gggcctttaa caatgctgtt gatacttttt
     6301 gtaattctgc tgtttgctct tccagattat gcaccctggg ttctagcgat agctggctgc
     6361 cacctacttt gtaatctaaa acccctacgc aatagatcgc cacaagaggt agtaaagtcg
     6421 caacgcagac agcaaaagct ggagccgaat ttaacgcaca accgacataa aatgacacgc
     6481 acagaaatat gagaagaaat gcaataattg ctaataaatt tcgtttattg ttcatataag
     6541 tcctggaatg agtcattagc ttggcgggta tgggtcgtga tctaattatt tacgccatag
     6601 cgaaaaaggt gcttttattg tggaaacaat ttactggggt cttgtatggg ttttgatttc
     6661 tgcgcttact gtgtgcgtgc taccagataa gacagaaacc caagacaaag aaccgaacca
     6721 gtccagtaaa gaaaaatcat agcaaaagag tgcatgggat tcttaccttt gaatatttta
     6781 tgatcccggc caatttcagc tccaacgcaa atcataaata caccgaagac gacaatagtg
     6841 agcagtagag cttcatacat ggccacgctt acaaacaaat ctggcttgaa aaagaaagtc
     6901 caaaccgttc cagttatagc tgcgaggatt aggcctggaa taattttatc gaacagcata
     6961 cggctaacta ctcatggtgg ttttgctcat cctacatggg atgaaaaagg ttttttcggt
     7021 tacgcggaaa gggtcgtgat cgaataaata ttgctttcta acttatagga gttattccat
     7081 gattgttgaa cggttgccat ttgaaagtga gcaaggcgaa gcaacgccaa aaagtttttt
     7141 gtccgccgct ttgcatgaac tagaagagaa caaccttttg gggaatgtaa gcgtgtatct
     7201 aatttgcgct atggaagccc ttgatcgtgg ggtcgcctac tcagaatcat ttatctgtaa
     7261 agatgagatt gaacgatgca aggaggttct caatcgttct aagcgttgac tcggcagttc
     7321 tggcggaatg agaacaatgt atctatcacc agcaccagtg aaaggtatgc catactcatc
     7381 aaaaatggct ttaatgtcag ctctatttga ctcgtttaca ccggaaataa atttgcctcc
     7441 aatgtgcatc ttatactctc tttgcggtta ggaatcgtca atatctaacc tatcaaattt
     7501 gagtaagtaa tcctagcccc tatagtcttg cccaacaaag catcaatccg gtccatagtg
     7561 tgaatcttca cgttaccctc atgtagtcga gctgtgactt catccaggta acgctgaa
//
LOCUS       DAOQGS010000150         7599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4640_length_7599_cov_4.156813, whole genome shotgun sequence.
ACCESSION   DAOQGS010000150 DAOQGS010000000
VERSION     DAOQGS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4640_length_7599_cov_4.156813"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKZ52_07430"
     CDS             <1..471
                     /locus_tag="PKZ52_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59643.1"
                     /translation="NNRDKALLTTMMQIRRYLYGGLLHREMSAYIKGRRKTLYFKGVM
                     SFYPLLNDVNQLRELDGWLISAIDRCIQARSRQLLSHGFDRRHSFPFNVKRKDILAKY
                     RSKLISGKPLLEVPSFKLIFRALERGLVEAGIERVMHPESSKYGYRDNYHSLHR"
     gene            677..1675
                     /locus_tag="PKZ52_07435"
     CDS             677..1675
                     /locus_tag="PKZ52_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNG59644.1"
                     /translation="MRLVASIVHSDDISAVIRQFYDLGSIKQCTFISQGINDTYLIKT
                     DLGKFIARVYRHLWRNRAQIDSEIDFVTYLAARAAPVAQFVAAQDGGYLHTLQCPEGE
                     RFLALMHCASGSEYETHAIPQNSPFNYGKAVGLLHSAASGFTAKNSCRLLDTHALIWQ
                     PLHALEEHFAEHTSTLDYLRSLASQLTKQIETLDAAGLTSLFIHGDLTGGNGNQNATG
                     SYMLFDFDCCGLGWQAYELAVFLWSLLQNNKAHLWSAFLDGYRSATILNNNDEQAIGL
                     FVAARSFWIMGYSLSRIAVLGSHSYRYVHFARDVEFIKQLKQALPNNMYIDWIYGN"
     gene            2055..2513
                     /locus_tag="PKZ52_07440"
     CDS             2055..2513
                     /locus_tag="PKZ52_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG59645.1"
                     /translation="MMNLRPAQHGDTEQIKHVVFSVLKEYGLTPDSKSTDQDLDAIEA
                     HYFLHNGFFGVVEFEGNIVATVGLHRVDGTTCELRKMYCLPQHRGRGLGRFLIEFALT
                     KACELGYTRIILETASPLKEAISLYKKYGFQEFTPEHLAARCDQAFELYL"
     gene            complement(2718..3734)
                     /locus_tag="PKZ52_07445"
     CDS             complement(2718..3734)
                     /locus_tag="PKZ52_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HNG59646.1"
                     /translation="MGPRLVRVAVLGGGPAGAAAAITLRQAGIEVMIIEPANFPRYRP
                     GETLHPGIEPLLHKLGVAPLLLNFGYLRHKGIWSAWHGAKQFVPYGEDANGPWYGFQA
                     PRGDFDQRLLNSACALGAQRIYAAAEGLIFDKTGVISGIQTSSEQVKADWVIDASGGA
                     HVLARFLKIVFTRFSPTLVARYGYRHGTCAEQEYPAIFADATGWTWIAEVEPNRYQWT
                     RVTQPHNRPASHWVPDSLLGLTAGPVYGADVTWRKAEQLAGEGWFLAGDAAAILDPSS
                     SQGVLRALMTGMMSAHLIIQSTAGHISRADCALVYQQWLNDWFAQDVLLMSQSYKVAG
                     LFGF"
     gene            complement(3719..5953)
                     /locus_tag="PKZ52_07450"
     CDS             complement(3719..5953)
                     /locus_tag="PKZ52_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59647.1"
                     /translation="MALIDDVKTVCDRLAPLGWRNVLLAASGGQLDIQKPTAAALKKE
                     LTKTLTAIDRTKPGFSDFALSGKKAITAGSPANSLLYHALASPGVTAGLSGFPTLNEI
                     EIVENFVFGVAPPSLANLHAKAGLTSAQKFSVVLFAYEYRPARDTCTGRQADLVFSRT
                     GVSRVGTREALYVPEVRGFLPRAEDDPFAFRVCPARFAAFLAVKVRGSAATIMRKQSA
                     DNNLGFWLPVHKLFAGTECIAGLNLHINYSAFHYNDKIRRTRSISLGLPAVPTTPPFQ
                     FSGGIAELENNSSMPPGWVMPVPHPRLVEPATLNGKLLTFKVPKGNKNFAALEPGSAT
                     GVDGSEIRPAPAYVHARSQVRNGEVVDLNNDPARPDVNNTVSKGGYEALHYVDFTGEG
                     QIDVAIPALNNLAEIAKTTVPCYSLVAAPDLFCSVGQRELFEAVPDDFWGVAPVPLCD
                     TRLPANLQLPGNKFSAKDKTMTALVALLGAPPSGGTLPVSQDAERHSALPDDCAGVFA
                     PGWDVSTDQLQVAGSPVQHLAAYGLGSPFPEDAKLCAALSTFWPTAAPDASRSMSPNT
                     GNAALRATVAPLTDEEIGQIGTLPWDGVVGPKIITINGKEFLDCEKFLHVDYVKNALN
                     NRFSPRLTSQVSSSEFKQRMQAIARVYDILGGNRNQCFVISFRKVSKGDPELQQAQID
                     GATVLRGDIYRIDLIRGSVEQAHPTDFRRSLLPIRNRQLFFTAPQNGTTLTRPAQVAK
                     WARA"
     gene            complement(6213..6467)
                     /locus_tag="PKZ52_07455"
     CDS             complement(6213..6467)
                     /locus_tag="PKZ52_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59648.1"
                     /translation="MVIFVVTKDGFRELEVIIKTGFYPVWVGANVLSEHELGRYRNMD
                     LDITNFSYEIDPADSEALTDALATIAEHHPGERVWLEYRP"
     gene            complement(6553..7218)
                     /locus_tag="PKZ52_07460"
     CDS             complement(6553..7218)
                     /locus_tag="PKZ52_07460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG59649.1"
                     /translation="MSRRTLNLDDRLYNYIVANSLREHPELATLRAATSSWPRANMQI
                     SPEQGQFMALLTQLSAAKRAIEIGVFTGYSALAVALALPADGYLLACDISAEDTDLGI
                     PHWQAAGVAHKIDFVVAPAVATLTQRVEQGEANSYDFAFIDADKTAYDSYYELCLQLL
                     KPGGLIAIDNVLWSGQVALEQIMDADTEALKALNRKIHQDARVDMVLLPISDGLTLVR
                     KRG"
BASE COUNT         1937 a         1944 c         1889 g         1829 t
ORIGIN      
        1 aacaaccgtg ataaggcatt gttaaccacc atgatgcaaa ttcgccgata tttatatggg
       61 ggcctactcc accgtgaaat gtccgcctat atcaaaggaa ggagaaaaac actttatttc
      121 aagggtgtaa tgagttttta tccactactc aatgatgtca atcaactccg cgaactagac
      181 ggctggctta tttcggcaat tgaccgatgc atccaagctc gatctcgaca gcttttgtct
      241 cacggctttg ataggcgtca ctcgtttccc ttcaacgtca agcgtaagga cattttggct
      301 aaatatcgga gtaaattaat ctcaggaaaa ccgcttttgg aggtaccaag cttcaaatta
      361 atctttagag ctttagaacg cggattagtc gaagccggta tagagagggt tatgcacccg
      421 gagtcgtcga agtatggcta tcgcgataat taccattcac ttcaccgttg acttccgatt
      481 gcagccctaa agggcaaccc gtaagttggg tcaactgtag ggcgggttcg cgaagcaacc
      541 caccatcctt tttgccgcga caatacttta tcgtaaacat tgaaatcgag gtctagaaat
      601 ggtggattgt cgcttcgctc cgaatccacc ctacgcctcg atccgctaaa tttaaatatt
      661 tgaacagggg tgatacatga ggttagttgc cagcattgtg cactcagacg atattagcgc
      721 tgtaataagg caattttacg accttggctc aattaaacaa tgcacgttta tttcccaagg
      781 catcaatgat acctacctca ttaaaaccga cctaggcaag tttatagcca gagtgtaccg
      841 ccacctatgg cgtaacagag cgcaaataga cagcgaaata gattttgtaa catacctagc
      901 cgcccgcgca gctccggttg cgcaatttgt cgccgcccaa gatggtggct acctgcacac
      961 attgcaatgc cccgagggcg agcgcttttt agctctgatg cactgtgcat caggttcaga
     1021 atacgaaacc cacgccatac ctcaaaatag tccatttaat tacggcaaag ccgttggatt
     1081 gttacacagt gctgccagcg gatttaccgc taaaaattcc tgccgcttac tggatacaca
     1141 cgcgttaatt tggcaaccct tgcacgcact ggaggaacac tttgccgagc acacatctac
     1201 ccttgattat ctgcgcagtt tagccagcca attaaccaag cagattgaaa ccttggatgc
     1261 tgctggccta actagcttat ttattcacgg cgacttaacc ggcggcaatg gcaaccaaaa
     1321 tgctaccggc agctatatgc tatttgattt tgattgctgc ggcctcggtt ggcaggccta
     1381 tgaattagcg gtatttctat ggtcactgtt gcaaaacaat aaagcccatc tgtggagtgc
     1441 ctttcttgat ggttatcgct cggcgacaat acttaataac aatgatgagc aggctatcgg
     1501 gttgtttgtg gccgcccgca gtttttggat tatggggtac agcttgtcgc ggattgctgt
     1561 gctgggaagc cacagttata ggtatgttca ttttgcgcgg gatgttgaat ttataaagca
     1621 gctaaagcag gcgctgccaa ataatatgta tatagattgg atttacggca attgattatt
     1681 aactgcggta ggttgggcag caatgcccaa cattccaaca ccccgtaatt aaatttatta
     1741 ttcaaaacaa ttagttgttg ggcattactg cccaacctac aacgcccaga ctgtttcgct
     1801 aaattaaatg caaccttcag aacgagtaac ccgtaaaaaa ctgtaagata ggtcaattgt
     1861 accccgcaag gtcggccaag ggtaaggtgg tctcacgaag caagccacca tcgatatcgc
     1921 tacggagaaa ttgtttcgac ctttgaagtt ggggccgcaa attggtggat tgtcgcttcg
     1981 ctctgaatcc accctacggc ctatttcaat aaaccaccac acaacaacag aattatcacc
     2041 acaacaagga gtaaatgatg aatttaagac cagcccaaca cggcgatacc gagcaaataa
     2101 agcatgtagt tttctcggtt cttaaagaat atggcctaac cccagactcc aaaagcaccg
     2161 accaagattt agacgcaatt gaagcgcatt attttttaca taatggcttt tttggtgtgg
     2221 tggagtttga aggtaacatt gttgccacgg tgggtttaca cagggttgac ggcactacct
     2281 gtgaacttag aaaaatgtac tgcctacccc agcatagagg gcgcggctta gggcggtttt
     2341 taattgagtt tgccttaact aaagcctgtg aacttggcta cacacgcatt attttagaaa
     2401 ccgcatcgcc acttaaagaa gctattagcc tatataaaaa atacggtttt caagaattca
     2461 cgccggagca tctcgccgcc cgctgtgatc aagcatttga gctttatcta tagcgaactg
     2521 ggagattgtc gcttcgctca gaatccacgc actatttgtt gggcattact gcccaaccta
     2581 cagctcaggc acacctaatc caagaaatgc tggataagcc actaccaccc catcaagcgt
     2641 aaaaacaaaa atacaattaa ctataaaata tttaccgacg gcaaaaaccg ccattgcctg
     2701 caacccccaa ttcagaacta aaacccaaat aacccagcaa ctttataaga ctggctcata
     2761 agcaaaacat cctgggcaaa ccagtcatta agccattgtt gatagaccaa agcacagtcg
     2821 gctcggctga tgtgccctgc ggtagactga ataattaaat gggcgctcat catgccagtc
     2881 attagcgcgc gcaatacgcc ttgggaggag gatgggtcca gaattgccgc cgcgtccccg
     2941 gccaaaaacc aaccctcccc tgccagctgt tcggctttgc gccaggtaac atcggcgccg
     3001 tacaccggcc cggccgttaa acccagtagt gaatcgggaa cccaatggga ggcggggcga
     3061 ttgtggggct gggttacccg cgtccattga taacgatttg gctccacctc ggcaatccag
     3121 gtccaaccgg ttgcatccgc aaagatggca ggatattcct gctcggcaca tgtgccatgc
     3181 ctatagccgt agcgggcaac cagcgtgggt gaaaagcgag taaaaacaat tttgagaaac
     3241 cgcgccaaca catgtgcgcc gccgctggca tcaataaccc aatcagcctt tacctgctcg
     3301 ctactggttt gaatgccgga tataacaccg gttttatcaa atattaaccc ttcagctgca
     3361 gcataaatgc gttgtgcgcc cagggcgcag gcgctattta atagacgctg atcgaaatcg
     3421 ccgcgcggtg cctgaaagcc ataccaaggg ccgttggcat cttcgccgta gggtacaaat
     3481 tgcttagctc catgccaggc cgaccaaatg cccttatggc gtaaataccc gaaattaagc
     3541 agcaagggcg caacaccaag tttgtgcagc agaggctcta tgcctgggtg cagggtttcg
     3601 ccggggcgat aacgggggaa attggccggc tcgataatca tcacctcaat accggcctgc
     3661 ctaagtgtta tagcggcggc agccccggcg gggccgccgc cgagcacagc aactctcact
     3721 aggcgcgggc ccacttcgcc acctgcgcgg ggcgcgtgag ggtagtgccg ttttgcgggg
     3781 cggtaaaaaa caattggcgg ttgcgaatgg gcagcaggct gcggcgaaaa tcggtggggt
     3841 gcgcctgctc aacactgccc cggattaaat caatgcggta gatatccccg cgcagcaccg
     3901 tcgctccatc tatttgcgcc tgctgtaatt cagggtcgcc cttactcact ttgcgaaagg
     3961 aaatgacaaa acactggttg cgattgccgc cgagaatgtc ataaacccgc gcaatagctt
     4021 gcatgcgctg tttaaattcg ctgctgctga cctgggaggt aagcctgggt gaaaagcgat
     4081 tattcagcgc gtttttaaca taatccacat gcaggaattt ttcgcagtcg agaaattctt
     4141 tgccattgat cgtaataatt tttggcccga ctacgccatc ccaaggcaga gtgccgattt
     4201 gcccaatttc ttcatcagtc agcggcgcca cggttgcgcg cagagcggca ttgccggtat
     4261 tgggcgacat ggatcgcgag gcatctggcg ctgcggtggg ccaaaaggta ctcagggccg
     4321 cgcagagttt ggcgtcctcg ggaaatgggc tgcctaatcc gtaggcggct aggtgctgta
     4381 cggggctgcc cgccacctgt agctgatcgg tgctcacatc ccagccgggg gcaaaaaccc
     4441 ccgcgcagtc atccggcaat gccgagtggc gctcggcatc ctgtgacaca ggaagggtgc
     4501 cgccgcttgg cggcgccccc aagagtgcaa ctagggcggt catggtttta tctttggcgc
     4561 tgaatttatt ccctggtaat tgaagattgg cgggcagccg ggtatcacac aggggtaccg
     4621 gcgccacacc ccagaaatca tcgggcaccg cctcaaataa ttcgcgctga ccaaccgagc
     4681 agaagagatc cggcgccgct actaatgaat aacaaggcac agttgtctta gcaatttcag
     4741 ccaggttatt taatgcggga atggctacat caatttgccc ctcgccggtg aaatccacat
     4801 agtgcagcgc ttcataaccg cctttggaga cggtgttatt tacgtctggc ctggccgggt
     4861 cgttatttaa atccaccacc tcgccattgc gcacttgcga gcgggcgtgc acataggctg
     4921 gcgctgggcg gatttcggag ccgtccacac cggtggcaga gccaggctcc aaggcggcaa
     4981 aatttttatt gcccttgggc actttaaagg tgagtaattt accgttgagg gtggctggct
     5041 ccaccaggcg cggatggggc actggcatca cccagcctgg cggcatggaa gaattgtttt
     5101 ccagttcggc aattccgcct gaaaattgaa agggcggcgt ggtgggtacg gcgggtaagc
     5161 ctaaggatat agagcgcgtg cggcgaattt tgtcgttgta gtgaaacgct gagtaattga
     5221 tgtgtaaatt aagcccggcg atacattccg taccggcaaa cagtttatgc accggcagcc
     5281 aaaaacccaa attattatcg gcgctctgct tgcgcataat agttgccgcg ctgccgcgca
     5341 cttttaccgc aagaaacgca gcaaagcgtg ccgggcagac gcgaaacgca aatggatcgt
     5401 cctcggcgcg gggtaaaaaa ccgcgcactt cgggcacata cagcgcctcg cgggtgccca
     5461 cgcgggagac tccggtgcgg gaaaatacca aatccgcctg gcgcccggtg caggtatccc
     5521 gcgccggtcg atattcatag gcaaacagca ccacggaaaa cttttgtgca ctggttaagc
     5581 ctgctttggc atgcaaattt gccaagctcg gcggcgccac accaaataca aaattctcta
     5641 caatttcaat ttcattcaga gtgggaaaac cactgagacc tgcggttacg ccgggagatg
     5701 ccagggcgtg gtagagcaaa ctattagcgg gggagcctgc agtaatagcc tttttgccgc
     5761 tgagggcaaa atcgctaaaa ccgggtttgg tgcggtcaat tgcggtgagg gttttggtta
     5821 attctttttt aagtgcagca gcggtgggtt tttgaatatc caactggcca ccggatgcgg
     5881 ccagcagaac attgcgccag cccagtgggg ctaagcgatc acaaaccgtt tttacatcgt
     5941 caataagtgc catagtggta accctataga aagcttgatg gtgtgataaa tcgttaaact
     6001 aggctaaaaa tatttgcggg cagcgaactt attaatccgg cggaaaaata gatcgtcctg
     6061 atctattttg ccttcgctgc gttaaactta cgaaaatgcg cggattttct tgttttaacg
     6121 cagctcgcgg cggcttgcac cgccgagcac agcatccttg ccgcgcttat taacccggca
     6181 ccaaatgccg ccgggttaat atctcggtaa atttatggcc tgtattccag ccaaactctt
     6241 tcaccagggt gatgctcggc gattgttgca agagcatcag tcagcgcttc actatctgca
     6301 ggatctattt catagctaaa attagtaata tctagatcca tattcctata gcgaccaagc
     6361 tcatgttcac tcaaaacatt agcgccaacc catacaggat aaaaacctgt tttaattatt
     6421 acctcaagct cacgaaagcc atcttttgta actacgaata taaccataaa aattcacaag
     6481 gcttttaagc gtctatttga atcattattt accagttttt attagccaca tgaagcgtgt
     6541 acaccgcact gatcaaccgc gcttgcgcac cagagtaagc ccatcactaa tcggcaataa
     6601 caccatatcc acccgcgcat cttgatgtat tttgcgattg agcgctttaa gagcttcagt
     6661 atcggcatcc ataatttgct ccagcgccac ctggccactc cacaacacat tatctatagc
     6721 gattaacccg ccgggtttaa gtagttgcaa gcacagctcg tagtagctgt cataggccgt
     6781 tttatccgca tcaataaaag caaaatcgta gctgtttgct tcgccttgtt cgacgcgttg
     6841 ggtgagggtt gcaacagccg gtgccaccac aaaatcaatt ttgtgggcca cgcctgcggc
     6901 ctgccagtgg ggaattccca ggtcggtatc ttcagcgctg atatcacagg ctaataaata
     6961 gccgtccgcc ggtagcgcca gggcaactgc taaggcgcta tagccggtga atacaccaat
     7021 ttcaatggcc cgtttggcgg cgctcagttg tgtaagcagc gccataaatt gcccctgctc
     7081 cggggatatt tgcatgttcg cgcggggcca gctgctggtg gctgcacgca gcgtcgcaag
     7141 ctcagggtgc tcgcgcaggc tattagccac tatatagttg tatagcctgt catccaggtt
     7201 aagtgtacgg cgggacataa ttgcttactc tgttagcggt ttggcccata gtagcccagc
     7261 tgtggccagg cgtcagctta ggcgctgatt aatgtccgtt ttggggtaac gattaactgg
     7321 ctttttaacc tgattctcgc gctggctaga taccatccgg cgagtgtttg agtgctgcaa
     7381 accttgcagt tatttatgca gattatttca aagacgcagc aaacacatcc atgtggtcgt
     7441 atcaacaggg tgacgatatt actccgaggt gcgtcattgt attattaacc cggtggcttt
     7501 ttatgccggg ttaataactg cggcaaggat gccgcgccgt cggcgcaagc cgccgcgagc
     7561 tccgctaaaa caagaaaatc cacgcatttt cgccagttt
//
LOCUS       DAOQGS010000151         7580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4661_length_7580_cov_4.143256, whole genome shotgun sequence.
ACCESSION   DAOQGS010000151 DAOQGS010000000
VERSION     DAOQGS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4661_length_7580_cov_4.143256"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKZ52_07465"
     CDS             complement(<1..870)
                     /locus_tag="PKZ52_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNG59650.1"
                     /translation="MTDTCTAPSAAQGLSSIEASRRLSAEGPNQLPKPNQRNFVRILR
                     DVVQEPMFALLIAGGVIYWLLGDTLEAVLLLIFASISVTITLVQESRSERVLESLRDL
                     ASPRALVLRDGRRQQVAGTEVVRGDLLIINEGDRIAADARLLSADDLLVDESLLTGES
                     VAVGKNPLDAANPEQSCVYAGTLVVRGTAMAEVSAIGLHTQMGHIGVALGSIDTEQAH
                     LQKQLRWLVRDFALLGALAALAVVVLYGLLRGSWLEAILGGIAIGMSLLPEEFPLVMA
                     VFMAMGAWRISQAR"
     gene            complement(1236..1928)
                     /locus_tag="PKZ52_07470"
     CDS             complement(1236..1928)
                     /locus_tag="PKZ52_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59651.1"
                     /translation="MLQLILIVGLIAAAYYHFKIKPKPAAERKKMLWLVAMWALAAIL
                     LVGVFTGKVHWLGAAAAAAFALLRTGFFTGLTLGRFWLAKNGGVARFSTEHIEAQLDV
                     NQLNLNGHIKKGPYANYSLDQLDKSQLEELLAYYQTLDKKSYYLIKTLLQRRGFYGEQ
                     PRQEPTPPGVSDLQEAMDILGLTGTPNKSDVLAAHRRLIHKLHPDRGGSDYLASQVNA
                     ARDQLLKYLEKM"
     gene            complement(1930..3408)
                     /locus_tag="PKZ52_07475"
     CDS             complement(1930..3408)
                     /locus_tag="PKZ52_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNG59652.1"
                     /translation="MPKVANPYFAAVDLGSNSFHLLIMRLQQGVLEVVDREKDMVQLA
                     LGLTPSGGLTRAAQKRALTCLKRFGERLASIPPAQIRVVGTKTLRAADRAKGFIKAAE
                     ETLGHTIEIISGFEEARLVYNGLAHSVANDNYNRLVIDIGGGSTELIIGRDATPHIME
                     SLNIGCVSASQLIGKEKVSKKSYQRAYLAAQAQIEQVRPNYLNAGWDIVYGTSGTMKA
                     IAEVLADTQGNAIIYKDALLALARVLIEDGEIHSPKLNAAREFVLPGGIAVLHALFDE
                     LNIDKIHVADATLKEGLIYDLVGRFDNEDARPQSVERLQKQYFVDTQQAQRVANGALA
                     LWRQLNTPTIPAVSRTKLLTWAALLHEVGLAISHSGHHHHGHYILQNSDLAGFGRYEQ
                     NLLAEMVRGQRKKMLSDGLDYIEPDTLAALLPLLVCLRLAVLLHRRREDLPFVPKLIQ
                     RGPQRFYLTFPTQWLKQHPLTTAGLEIEANYLAAAGLHLGFK"
     gene            3549..4163
                     /locus_tag="PKZ52_07480"
     CDS             3549..4163
                     /locus_tag="PKZ52_07480"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNG59653.1"
                     /translation="MRNLFGLHLLTAQDARIRQLKRTHRCAVHGQKVWRSASVLLDYL
                     QEYPLAKGLRVLELGAGWGLAGIYCAKYAAAAVTALDIDPAVLPYLQLHAHLNEVAIT
                     PWTADFTELSAAQLSQFDVIIGSDICFWDQLSEPLLQLINRAQQLNIRAIIADPGRPP
                     FIEVIETLLDQPAGKTLAVENWSVPHPYNVHGVILDTGAPYVEL"
     gene            4150..4797
                     /gene="msrA"
                     /locus_tag="PKZ52_07485"
     CDS             4150..4797
                     /gene="msrA"
                     /locus_tag="PKZ52_07485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNG59654.1"
                     /translation="MWSFERKKQQMVSPEAALPGRTEAMPIKGQHSVLGLPLTPPYPS
                     ECQQILFGMGCFWGAERVFWNLPGVHVTAVGYSGGYTPNPLYEEVCSGLTGHNEVVLV
                     VYRPRDVSVEQLLQVFWQSHNPTTPMQQANDIGTQYRSGIYCYEQAQLAAAQASKEQY
                     QALLGEQLAITTEIKMAEAFYFAESYHQQYLAKNPGGYCNLQTVAKTGLPTFEPK"
     gene            complement(5460..6764)
                     /locus_tag="PKZ52_07490"
     CDS             complement(5460..6764)
                     /locus_tag="PKZ52_07490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG59655.1"
                     /translation="MTTPLRPLKILLVEDSPTLRHVLTGDIRHAGHEVIIAQSGEEAL
                     QIVESTTVDMILMDVEMPGLDGFETTRLIREMLFNHWIPIIFVTGKDNDEDLAQGIDA
                     GGDDYLIKPVRRVILQAKIRAMERIIAIRDQLRHVNEELRDLSERDSLTKLYNRRVFQ
                     DRAEERWKQSVRSHEPIAILLLDIDHFKAYNDCYGHVAGDDCISQVANAIARCVNRPG
                     DVLARYGGEEFIALLHNTGEAGARHVAESIRESVIALRIPHQDSQTADHVTVSIGGSV
                     IHITAGTTLLGQISSADKLLYAAKQNGRNRCVIKTFNPQNTLLVVDAHGGIVDAVDRA
                     LHGQFSLLSANNVEAALAQIQETQPDIIVLDAQLGGNNALRLVQKLRTQAETAQIPIL
                     WVTEYASDFVSLSHNQNYRIDGFINKPIDGNKLVAKVDSLLK"
     gene            6964..>7580
                     /locus_tag="PKZ52_07495"
     CDS             6964..>7580
                     /locus_tag="PKZ52_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNG59656.1"
                     /translation="MSEFDDIRPYNDDEVKPTLERLLNDSEFLDTIASLKLPAWLKSI
                     RPLVRPLIKRRLSREVSGISSVFAFQQRIEYYMGQMLEKTITHLTISGLDRLDKNSAY
                     CFISNHRDISMDPAFVNWALFHGGYQTLRIAIGDNLLTKPFASDLMRLNKSFIVNRSA
                     TAPREKFKVAKKLSHYIQHSMHEDQANIWIAQREGRAKDGNDKTNP"
BASE COUNT         1795 a         1958 c         1989 g         1838 t
ORIGIN      
        1 ccgggcctgg gagatgcgcc aggcgcccat ggccataaac accgccatta ccaaggggaa
       61 ctcttcgggc agcaatgaca taccaatggc gatgccgcct aggatggcct ccagccatga
      121 gccccgcaag aggccatata acaccaccac cgccagggcc gctagggcgc ccaacagggc
      181 gaaatcccgc accagccagc gcagttgttt ttgcaggtgg gcctgttcgg tatcgatgct
      241 gcctagggcc acacctatgt ggcccatttg cgtgtgcagg ccaatggcgc tcacttcggc
      301 catggctgtg ccgcgcacca ccagtgtccc cgcataaacg cagctctgtt ctgggttggc
      361 cgcatccagc gggttcttac ccactgccac ggactccccg gtcaagagcg attcatccac
      421 caataaatca tccgcgctga gcaggcgggc atcggcggcg atgcgatcgc cctcattgat
      481 aatcagcagg tcgccccgca ccacctctgt tcccgccacc tgctggcgcc tgccgtcgcg
      541 cagcaccaag gcgcgggggc tggccaaatc ccgcaaactc tccagcaccc gctcgctgcg
      601 ggattcttgc accagggtaa tcgtcaccga aatggaggcg aatataagca gtagcacggc
      661 ttctagggtg tctcccagca gccaataaat caccccacct gcaattaaca gcgcgaacat
      721 gggctcctgc accacgtccc gcagtatgcg cacaaaattg cgctggttgg gtttgggtaa
      781 ttggttgggg ccttccgcgc tcaagcggcg ggaggcctca atgctgctaa ggccctgggc
      841 agcagaaggc gcagtgcagg tgtcggtcat ggaaaggccc tttaggattt gcctacacca
      901 tagcacctac acagtgatgc cgattgactc ggatcaggca tttacctgcc gtgaggtttt
      961 taggattaaa aactcaggta tttttttcgt agctattcag acacctttat tgtcagcaca
     1021 ccttatcgtc agtacacctt attgccctct taagcgcggg tggcaaatta aggaagattt
     1081 agccaagcag cagaatcaat tcacggcgtg tctgaaataa cctgtattca ttactgcggg
     1141 gagtgactta gagctcacag cacccaagca tttgcctggt gatgtctgtc gggcgcggga
     1201 atcccgtcca gcttaaataa aatggtgctg aatagttaca ttttttccag atatttcagt
     1261 agctgatccc gcgccgcatt gacttggctg gctaaataat cgctgccgcc gcgatccggg
     1321 tggagtttat gaatcagcct gcggtgagcg gccaatacat cgcttttatt gggggtgccg
     1381 gtaaggccta aaatatccat ggcctcttgc agatcggaca cacccggcgg tgtgggttcc
     1441 tgtcgaggtt gctcgccgta aaaaccgcgc cgctgcaaca gcgtttttat gaggtaatag
     1501 gattttttat ccagggtttg ataataggcg agcaattcct ccaattggct tttatccaat
     1561 tgatcgagac tgtaattcgc ataggggcct tttttaatgt ggccgtttaa atttaattga
     1621 ttaacatcca attgggcctc tatgtgctca gtggaaaaac gggccactcc accatttttg
     1681 gccaaccaaa aacgccccag ggtaaggccg gtaaaaaaac ccgtgcgcag cagtgcaaaa
     1741 gcggcggcgg ccgcagcgcc cagccaatgc actttgccgg taaatacccc caccaataaa
     1801 atagccgcca gcgcccacat ggcaaccagc cagagcattt ttttgcgttc agcagcgggt
     1861 ttgggtttga ttttaaaatg gtaataagct gctgcaatta aaccgacaat taaaatgagc
     1921 tgcagcatat tatttaaatc ctaaatggag ccctgcggcg gctaaataat tagcctctat
     1981 ttcaaggcct gcggtggtaa gcgggtgttg ttttagccat tgagtgggga aggttaaata
     2041 aaagcgctgg gggccgcgct gaataagttt gggcacaaag ggtaaatcct cccggcggcg
     2101 gtgtaaaagc acggccaaac gcaaacagac taacagcggc aataaagccg cgagggtgtc
     2161 cggctcaata taatcaagcc catcgctgag catttttttg cgctggccgc gcaccatctc
     2221 cgctaatagg ttttgctcgt agcggccaaa acccgctaaa tcactatttt gcaaaatata
     2281 atggccgtgg tggtggtggc cggaatggga aatggccagg cccacttcat gtaataaggc
     2341 cgcccaggtt aataatttcg tgcgggagac ggcgggaatc gtcggtgtat tcagttgccg
     2401 ccagagggcc agggcgccat tggctactcg ctgggcttgt tgggtatcga caaaatactg
     2461 cttttgcagg cgctccacgc tttgcgggcg ggcatcttcg ttgtcaaaac ggcccactaa
     2521 atcgtaaatt aaaccttctt ttaaggttgc atcggcaacg tgaattttat cgatatttaa
     2581 ttcatcaaac agcgcatgta acaccgctat gccaccgggc aaaacaaatt cccgcgcggc
     2641 atttaatttg ggggaatgaa tttcaccatc ttcaattaat acccgggcca acgccagcag
     2701 ggcatcttta taaataatgg cattgccttg ggtgtctgct aacacctcgg caatggcttt
     2761 catggtgccg gaggtgccat acactatgtc ccaaccggca tttaaataat tgggccgcac
     2821 ctgttcaatt tgtgcttgcg cggcgaggta ggcgcgttgg taggattttt tgctgacttt
     2881 ttctttgcca attaattggc tggcgctgac acagccgata tttaaactct ccataatatg
     2941 cggggtggcg tcgcggccaa taatcagctc cgtactgccg ccgcctatat caattaccag
     3001 gcggttgtaa ttgtcattgg ccaccgagtg ggcaaggccg ttgtacacca gacgggcctc
     3061 ttcaaagccg gaaataattt cgatggtgtg gccgagggtt tcctccgccg ctttaataaa
     3121 gcccttagct ctatccgccg cccgcagggt tttggtgcct accacgcgaa tttgcgccgg
     3181 tggaatactg gctaagcgct cgccaaaccg ctttaaacag gtgagggcgc gcttttgcgc
     3241 ggcgcgagta agcccgccgc tgggggtgag ccccaaggcc agttgcacca tgtctttttc
     3301 ccggtcaact acctctaaaa ccccctgttg cagccgcata attaaaagat gaaaactgtt
     3361 ggaacccagg tccacagcgg cgaaataggg gttggcaact ttgggcatag gcaaaccaat
     3421 gcaaaggggg aggcgggatt atgccaaggc ggccgcccat tgcctaccgc tttagcagcg
     3481 ctttcataaa caccttgctc tgctgggcgt attcagagtg taatcgagtt ttttattggg
     3541 gagcgcccgt gcgcaatctg ttcggcctac acctactcac cgctcaagac gcgcgaattc
     3601 gccagttaaa acgcactcac cgctgtgcgg tgcacggcca aaaagtttgg cgcagcgcca
     3661 gtgtgttgtt ggattatctg caggaatacc cccttgcgaa gggcctgcgg gtgttagagt
     3721 tgggcgcagg ctgggggctt gccggaatct actgcgcaaa atacgccgct gcggcggtga
     3781 cggccttaga tatcgaccct gcggtattgc cttatttaca gctgcacgcg cacttaaatg
     3841 aggtggccat caccccgtgg acagctgatt ttaccgagct gagcgcagca caattaagtc
     3901 aatttgacgt aattattggc agcgacattt gtttttggga tcagctgagc gagccgctgc
     3961 tgcagctaat taatagggcc cagcaattaa atatccgcgc gattattgcg gacccaggcc
     4021 ggccgccatt tattgaggtg attgaaacac tgttagacca accagcaggt aaaacccttg
     4081 cggttgaaaa ctggtccgtg ccccacccgt acaatgtgca cggtgttatt ttggatacag
     4141 gagcccctta tgtggagctt tgaacgcaag aaacaacaaa tggtaagccc tgaggctgcc
     4201 ctgcccggcc gcactgaggc catgcctata aaaggccaac acagtgtttt aggcctccct
     4261 ttaacgccgc cataccccag cgagtgccag caaatcctgt tcggcatggg ctgtttttgg
     4321 ggggcggagc gggtgttttg gaacttgccc ggtgtacatg tcaccgctgt cggctacagc
     4381 ggcggttata cgcccaaccc cttgtatgaa gaggtgtgca gcggccttac cggccacaac
     4441 gaggtggtat tggtggtgta caggccccgt gacgtgagtg tggagcagct gctgcaggtc
     4501 ttttggcaga gccacaaccc caccacgcct atgcaacagg ccaacgatat aggcactcaa
     4561 tatcgctccg gtatttattg ttatgaacaa gcccaactgg cggcggcgca ggcttctaaa
     4621 gagcaatacc aggcattatt gggggagcag ctggctatta ccaccgaaat aaaaatggcc
     4681 gaggcgtttt attttgcgga aagctaccac cagcaatatc tggcgaaaaa cccaggcggt
     4741 tattgtaatt tacagacggt ggcaaaaacg ggtttgccaa cattcgagcc taaataaccg
     4801 ctagcgtgtt taccgcttaa ttcggcggcg ctacctgagt atttattatg gggtggcgta
     4861 actatttagc cctttttatt gtagcgaggc tcgatgttcg cgccggctag aacaggcaag
     4921 tgctgttccc ccgcagtgat gaatacaggt tatttcagac acgccgtaaa tacatccgtg
     4981 taggctcgtc aaaaaagtcc ctttttttga cggtctgaaa taacctgtat tcaccactgc
     5041 tgcaccgcga atcgaaaatg ccacctgact aattccccct caatttgtct tccacgtggc
     5101 gagagtaata ttaacccggc ggcatttagt gccgggttaa taatggcggc atggatgcag
     5161 cactcggcgg tgcaaaccgc cgagtgctgc gcaaaaacaa gaaaatccac gtattttcgc
     5221 cagtttttgc gctgcgaagg ccaaatagat caggacgatc tatttggccg ccgggttaat
     5281 aaggtgcgct gaatagttaa taagtgttaa gggcttgcag caccacagcg ctcgcctaag
     5341 gttttccaga tggcctgaga atcacgtcca gctacaataa caaggtgctg agcaattaca
     5401 attttttacc caaatttcca ccagtgtggg agttgtaaaa acattctgag tgattatttt
     5461 tattttaata aactgtccac tttcgccacc aatttattac cgtcaatggg tttgttaata
     5521 aatccatcaa tacgataatt ttggttgtgg gagaggctga caaaatcgct ggcgtattcg
     5581 gttacccaca aaataggaat ttgtgccgtc tccgcttggg tgcgcagctt ttgcaccagg
     5641 cgcagggcat tgtttccgcc cagctgggca tccagcacaa taatatccgg ttgggtttct
     5701 tgaatttgtg ccagcgccgc ttccacatta ttggcggaga gcaaactaaa ttggccgtgc
     5761 agggcgcggt caactgcgtc cacaataccg ccgtgggcat ccaccactaa cagggtattt
     5821 tgcggattaa aagttttaat gacgcaacgg ttgcggccat tttgtttggc cgcatagagc
     5881 aacttatccg ccgagctaat ttgccctaac agggttgtgc cagcggtaat atgaatcacc
     5941 gagccgccga tgcttaccgt gacgtgatca gcggtttggg agtcctggtg gggaatgcgc
     6001 agggcgatca ccgactcgcg gatggattcg gccacatggc gggcaccggc ctcgccggta
     6061 ttgtggagca gggcgataaa ctcctcaccg ccgtaacggg ctaacacatc gccggggcgg
     6121 ttgacgcagc gggcgatggc gttggccacc tgggagatac aatcgtcccc ggccacatgg
     6181 ccataacaat cgttgtaggc tttaaagtgg tctatatcca atagcagaat ggcgatgggt
     6241 tcgtggctgc gcacggactg cttccagcgc tcttcggcgc gatcttgaaa tacccggcgg
     6301 ttgtacaatt tggtcagact atcccgctcg gataaatccc gcagctcttc attgacgtga
     6361 cgcaattggt cacgaatggc aattattcgt tccattgcgc gaattttcgc ctgcaaaatc
     6421 acccgccgca caggcttaat taaatagtca tcgccgcctg catctatgcc ttgggccagg
     6481 tcttcatcgt tgtctttgcc ggtgacaaaa ataatcggaa tccaatgatt aaacagcatt
     6541 tcgcggatca ggcgggtggt ttcaaaaccg tccaggcccg gcatttccac gtccattaaa
     6601 atcatatcca cggtggtgga ttcaacgatt tgtagggctt cttcaccgct ttgcgcgatg
     6661 atcacttcgt ggcctgcatg gcggatatcg ccggttaata cgtggcgcag cgtcgggctg
     6721 tcttcaacca gcaggatttt gagcggtctt aaaggcgttg tcattgcact tcacttaatt
     6781 aggattatat gcaaagtagg gcgggcagct taccacgtcg aggccatttg ccctgtgata
     6841 ttggcacata aatcggccta ctttgagccc gacgcgacga gtggtcggcc atgaaacggg
     6901 tggcaattag tgtgtctttt cggtatggtg tgacgccgtt tacctagcta attttgtgcc
     6961 gttatgtctg aatttgatga tattcgtccc tataacgatg atgaagtaaa gcccacctta
     7021 gaacgcctgc taaatgacag cgaattttta gataccatcg ccagcctgaa attgcccgct
     7081 tggttgaaat ccatccggcc attggtgcgc cccttaatta aaagacgctt aagccgcgag
     7141 gtaagcggta ttagcagtgt atttgcgttc cagcagcgca ttgaatacta catgggccaa
     7201 atgttggaaa agaccattac acacctgacc atttctggtt tggatcgatt ggataaaaat
     7261 agcgcttatt gttttattag taaccaccgc gacatttcta tggacccggc atttgtgaac
     7321 tgggccctat ttcatggcgg ttatcaaacc ctgcgcattg ccattggcga caatttatta
     7381 accaagccat tcgcttcgga tttaatgcgg ctgaataaaa gttttattgt taaccgttcg
     7441 gccacggcgc cgcgggagaa atttaaagtc gcaaaaaaat tgtcccacta tattcagcat
     7501 tccatgcatg aagatcaggc gaatatttgg attgcccagc gggagggccg cgccaaagat
     7561 ggcaatgata aaaccaaccc
//
LOCUS       DAOQGS010000152         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4664_length_7578_cov_4.368337, whole genome shotgun sequence.
ACCESSION   DAOQGS010000152 DAOQGS010000000
VERSION     DAOQGS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4664_length_7578_cov_4.368337"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKZ52_07500"
     CDS             <1..1041
                     /locus_tag="PKZ52_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HNG59657.1"
                     /translation="QEIVKQAYNYIYGEKPNPPELVTGSINGNTITVNVTDKGKSISF
                     TATLTTPSGNGPFPVMIGYGGGLFGSGIPAGLQTILTRHNVATISYDPYVLGKENGGS
                     GAKAGKFYDLYGTNHSAGLLVAWGWGISRIIDVLEQNNRVNAAKVGVTGCSRFGKGPF
                     IASAFDARIALSMPVESGIGGVPALRLVPRIDASGEQPYHAMSYERWLDPTVMRPFTT
                     SNATTGHNLNKLPIDTHSVIGLIAPRGLLVVDNPGIANLDPNSAWVASKAGQMIYEGL
                     GYGTNVGYVGAGGNHCEWRSQYSAPAEAFVRKFLKDETSVTTGSFTTSGTQQNVSNYV
                     NWTVPSLSGQLK"
     gene            1567..3975
                     /locus_tag="PKZ52_07505"
     CDS             1567..3975
                     /locus_tag="PKZ52_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNG59658.1"
                     /translation="MLNIKLLSSKYLFGSIFTVVVSQVFANTDTAYHAQLCEDAIGVS
                     IPAFNCRDVSSPGGSASILNGQTTTGGKCDKPAKLNGSCAPQSVGGVLFNSPDAKIYL
                     SCRSYNNGSTFSDVAVIAVNRNNGAACFFQTALGTPLTADQPRRPILPVGEGGEAGWF
                     ATGGSCLTCHSNGGLIRSPYMAATNDVAHQVHGTGVNYFYTDNTPLWNVILPEQEVYS
                     ISAVDDPNTQTNNEAYCTSCHRLSAVWDGSQFNTSGTSSHYALAAHADNYDGTSTGQI
                     TLTSGNFSKIYQPKNQGINSNKNPNSTTSPLWMYPGATSFSANTFTNAARVSFCTETS
                     TPSTNPSICTQPIKSPRPSDLGIWGNGLAKGKFGGETSSSISSLKNDADTTRKMFYPI
                     MSGYFNTDDSLDLAWREPVGTTGYKWVFYLGNKTGTFTLAPFSYTWNAARPANEFPFV
                     GDFNGDGKTDIGIRSNNQWIIKYNQGNGVFNGDTTFNTPTAIANDKLVVGDFNGDKKS
                     DILHWGSSASLIRKSLGNGQFDAGTPVIGMNGSASEAYAGDINGDGYADIGWLYLNTS
                     FTFFNTFDKDRTPSNFGNFESGAVYPVSDSKTTFLGDVNGDKLADFGELITISESGSN
                     SIKSVSVNYSVKGTEPRFIKSKLGECLTAVQPVPTSNDLYGPNSMPFVRKDKPGLITK
                     PCGSLTTFAEETSRQLFRIGLHGDFLAKIQSTVDQNPNVYLGRPFYLYANTSTTVQQA
                     DMRDITSAGNKTDWNIVYTNSDMKYFQLKSVSGGNCLQRNSSTSIVLAACNTSDAQKW
                     THLR"
     gene            complement(4189..4575)
                     /locus_tag="PKZ52_07510"
     CDS             complement(4189..4575)
                     /locus_tag="PKZ52_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG59659.1"
                     /translation="MDITNERRQFTRVGFAAEAELIQEGKMLRAELIDISLNGLLVRT
                     PDEYEFRTDIPCCVQIHLSDDTLIQMQVALVHSGSQCLGFHCTSIDMDSIIHLRRLIE
                     MNLNDPTAAERVLAELVGRQKYPQEL"
     gene            4760..5473
                     /locus_tag="PKZ52_07515"
     CDS             4760..5473
                     /locus_tag="PKZ52_07515"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNG59660.1"
                     /translation="MLDTKSHIETPEGVRLPISIASICPRAYAYGIDLFIRLIALAAV
                     SSVLGVLGKLGGGLMLLIMFSLEWFYPVVFDVFNQGATPGKRIMQLQVVHDDGSPVSF
                     SSSLLRNLLMVVDFLPVFYCLGTLVSICHPFSKRLGDIAAGTLVIHQSTASRHIHLDA
                     GLGKRAPFAGLSLPERRALVSFAERCPSLSQARQAELAGILAPLLPADCAEQAVLALQ
                     QMANQIAGASIPADKEPQP"
     gene            5470..6435
                     /locus_tag="PKZ52_07520"
     CDS             5470..6435
                     /locus_tag="PKZ52_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNG59661.1"
                     /translation="MKQADFERRGEPLWRAFAEELSVLEASARAVPSPQFAHHYRQIC
                     QAHALCERRQYTAQLEMQLADLVVRGQRYLYRRPTRFVQAFWRFVISGFPSAVRREAR
                     LFALVSALFYLPALGLAWALSFQPDLIYSLMPPQAVQHLENMYQPDNITLGEARDSGT
                     NWEMFGFYIRNNIGVAFRTCASGLLLGVGSLLLLLYNGLMLGGAAAHLTHLGYQQTFF
                     TFVIGHGSFELTAIVIAAVAGLKLGLALVAPGRLPRLQALRLAGAQAIVLAMGAGLML
                     VVAAFIEAFWSSNNLLPPGLKYAVGALGWVCVAAYLWFAGRRRGA"
     gene            6389..>7578
                     /locus_tag="PKZ52_07525"
     CDS             6389..>7578
                     /locus_tag="PKZ52_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59662.1"
                     /translation="MWLHTSGLRGAAVELERRAIEIRPRSAWEACDLGVQMGRHWWWP
                     MVKIWLALAAPWLLLSWLLPARFAFWPAFLYWLGKPLWERFLLYFLSQAIFTSIPSTA
                     QVLKNAPLLLAKDWLASLLWRRLSLWRAYNCALTVLEGQTGKARQQRLKHLAGDNAAT
                     ASKVALLLAHMEGFLLLSLLILAYTLAPRDLDWGFAQLHEWLASDTFAAGYNSLYILV
                     STLVAPFFVATGFALYLNRRIWLEAWDIDISFRTLAKRLSPALLLILLGSCLWAPGLA
                     PSAQSQAAQAQSQAAQPQTKDQQTSQISQALERLANPASLFSELQPAPELIAHYPAEQ
                     ARVAAVLAGEDFHRKETRLVHHWRWDWESTPAQDTDRARWEQVIAITARTLEVLLWAL
                     ALAL"
BASE COUNT         1840 a         1979 c         1877 g         1882 t
ORIGIN      
        1 caggaaatag taaagcaggc ctataactat atttatggtg aaaaacctaa tcctcccgaa
       61 ttggttaccg gctcaattaa cggcaacacc attaccgtaa atgtaaccga taagggaaaa
      121 agcatttcct ttacagcaac actgacaacc cctagtggta acggcccatt cccggtgatg
      181 atcggttatg gcggcggatt atttggctcg ggaatccccg cgggcttaca aacaattttg
      241 actcgccata atgtagccac catttcttac gacccttatg tattgggtaa agaaaatggc
      301 ggcagtggcg ccaaagccgg taagttttat gatctttatg gcaccaatca ctctgcagga
      361 ttgttggttg cctggggctg gggcatcagc cgtattatcg acgtattaga acagaacaac
      421 cgggtcaatg cggctaaagt aggtgtgact ggatgttctc gcttcggtaa aggccccttt
      481 atcgcttcgg cgtttgatgc gcgcattgcg ctatccatgc cggttgagtc gggtattggc
      541 ggcgtgccag cgttgcgctt agtgccgcgc attgatgcca gtggcgaaca gccctaccac
      601 gccatgagtt atgagcgctg gctggatcca accgtgatgc gacctttcac tacctctaac
      661 gccaccactg gccacaatct caacaaactg cctattgata ctcactctgt aatcggcttg
      721 atcgcaccgc ggggcttatt ggtagtggat aaccccggta ttgccaattt ggatcccaac
      781 tccgcttggg ttgcatccaa agctggccag atgatttatg aaggcttggg ttatggcact
      841 aacgtgggtt atgtgggcgc aggcggtaac cactgcgaat ggcgctcaca atacagtgct
      901 cctgcagaag cttttgtgcg taaatttttg aaggatgaaa cctcggttac taccggcagt
      961 ttcaccactt caggcaccca gcaaaatgtc agcaattatg ttaactggac ggtaccgagc
     1021 ttgagcgggc agttaaaata aaccgacagc taaaattgat atattttaat acggcgcgtg
     1081 agcgccgttt ttttgcacat tttttgaaaa caatagttat tcaataggtg ttaaattgca
     1141 taaagatgaa atctagcaag taaatccagg tagatttttt gattgattag agtgggcata
     1201 atgaagaatt acaagcgggc aacacacatc acagccctcg ccccacttcc aaacaccccc
     1261 ccgcatctcc tgaacctcac aaagagaata cagtatgagc acataaacac cgccatcaaa
     1321 tagtttaatg gcaagcggat tttattagca aatcaataat ataaataatt ttctcattca
     1381 ttgttaattc caattgaaaa ccagcttgaa tttctgactc accttgagaa aaaccaaatc
     1441 aattaaaata tttttacagt taagcattga aaacaaacac aacattaatt agcatgccgc
     1501 ccactattca acagaggaat agccaaagct ttttgcccat ctagattcca aaatacagga
     1561 cggatcatgt taaatataaa attactcagc tccaaatatt tatttggcag tatttttaca
     1621 gtcgtggtat cacaggtatt tgcaaatact gatactgcat accatgcgca gttatgtgaa
     1681 gatgcaattg gggtatccat tcccgccttc aactgccgag atgtttcctc accaggaggg
     1741 tctgcctcga ttttaaatgg gcaaacaaca acgggcggaa aatgcgataa gcctgcaaaa
     1801 ttgaatgggt cttgcgcccc gcaaagcgta ggcggtgtct tattcaatag cccagatgct
     1861 aaaatctatc tttcctgccg cagttataat aatggcagca ctttttcaga tgtcgctgtc
     1921 attgctgtta accgaaataa cggcgccgcg tgtttttttc aaactgcact tggtacacct
     1981 ttaacagccg atcaacccag acgcccgatc cttccagtgg gtgaaggggg cgaagcaggt
     2041 tggttcgcca ctggcggaag ttgtttgaca tgtcactcca atggaggatt gattcgttcg
     2101 ccttatatgg ccgcaaccaa tgatgttgcc catcaagtac atggaacagg tgtcaactat
     2161 ttctacaccg ataacacccc actgtggaac gttattttac ccgaacaaga ggtttattcg
     2221 atttctgcag tcgacgatcc aaacactcaa accaataatg aagcttattg cactagctgc
     2281 cacagattaa gcgcagtttg ggatgggagc caatttaata cttcaggcac ttcatctcat
     2341 tacgcgttag ccgcccatgc agataattat gacggcactt caactggtca aattacactg
     2401 acatcaggaa atttcagcaa gatttatcaa cccaaaaatc agggtattaa ttcaaataaa
     2461 aatcccaatt ccacgacatc cccattgtgg atgtacccag gagcaacgag cttttccgca
     2521 aacacattta caaatgcagc acgagtatct ttttgcacag agacttctac cccctctacc
     2581 aatccgtcta tttgcacgca accaattaaa tcaccaaggc catctgattt agggatttgg
     2641 ggtaatggcc tcgccaaggg aaaattcggc ggcgaaacat catcttctat tagctcgttg
     2701 aaaaacgacg cagatacaac acgaaaaatg ttttatccga taatgtccgg atacttcaac
     2761 accgacgatt cgttagattt agcctggaga gaaccagttg gcacaacggg atataaatgg
     2821 gttttctact taggcaacaa aacaggtaca tttaccctcg ctccattttc ttacacctgg
     2881 aatgccgcaa gacctgccaa cgaatttcct ttcgttggcg attttaatgg cgatggaaaa
     2941 acagacatcg gcattcgctc aaacaatcaa tggataatta aatataacca aggcaatggt
     3001 gtttttaatg gcgatacgac atttaatact ccaaccgcca ttgctaatga taaattagtt
     3061 gtcggcgact ttaatggaga caaaaaatct gacattttac attggggctc tagcgcttca
     3121 ctcatcagaa aaagtttagg caacggacaa tttgacgcgg gaacaccagt aattggcatg
     3181 aacggcagcg cgtcagaagc atatgctgga gatattaatg gcgatggtta tgccgacatt
     3241 ggctggctat atttaaatac aagctttact tttttcaaca cttttgacaa agacagaaca
     3301 ccttcaaatt ttggcaactt tgaaagcggc gccgtatatc ccgtctctga ttcgaaaacc
     3361 acgtttctcg gtgacgtaaa cggggataaa ttggccgatt tcggggagct cattacaata
     3421 tcagaaagcg gctccaatag tataaagtct gtaagcgtta actattcagt caaaggtaca
     3481 gagccaagat ttattaaaag taaattaggt gaatgtttaa ccgctgtcca accagttccg
     3541 acgtcaaacg atctttatgg cccaaattcc atgccctttg ttcgaaagga caaacctggg
     3601 ttaataacca agccgtgcgg tagtttgaca actttcgcag aggaaacgtc acgccaacta
     3661 tttagaatcg gactacacgg tgattttctt gcaaaaatac aatccacggt tgaccaaaac
     3721 cctaatgtgt atttaggccg ccccttttat ctctacgcca acacatcaac caccgttcaa
     3781 caagcagata tgagagatat tacaagtgcg ggcaataaga cggactggaa cattgtatat
     3841 acgaatagcg atatgaaata tttccaattg aaatccgtat cgggtggaaa ttgtttgcaa
     3901 cgcaatagtt caacaagtat cgttctagct gcttgcaata ctagcgatgc ccaaaaatgg
     3961 acgcatcttc gttgataaca atttaaattg ctataaagtc tgcgctccac tttaccctgg
     4021 gggcgcagat ttttccctaa ttgtctcccg ctcgaatatc attaagttaa gtcttctaat
     4081 taatatttat gctgctacaa aagcgtttcg ttttcatttt tctagggtgt gggcgtctag
     4141 cctttccgct tcgaccctag ctggaagctt gctaacaaag ccctcaaatt acaactcctg
     4201 cggatacttc tgtctgccca ccaattccgc caacacccgc tctgctgcgg tcgggtcgtt
     4261 aaggttcatt tcaattaagc ggcgcaggtg aataatgctg tccatatcaa tgctggtgca
     4321 gtgaaagcct aaacactggc tacctgaatg caccagggcc acctgcattt ggatcagggt
     4381 atcgtcggat aggtgtattt gcacacaaca gggaatatcg gtgcggaatt cgtattcgtc
     4441 gggggtgcgc accagcaggc cattgaggga gatatcgatc aactcggcgc gcagcatttt
     4501 cccttcttga atcaactcgg cctccgccgc aaaaccgacg cgggtgaact ggcggcgctc
     4561 gtttgttatg tccatggtgt actctcgtta ggcctggttt ttgtgctggt ataggataag
     4621 tgtaggcgcg gattggcaat tggaaaactt aacatagatc cttaagttgc cgggctgaga
     4681 ccaagctcgc taccatggcg gtcagcgcta ttcttctgga tgagcattaa gaacaggagc
     4741 cattattcgg aaccccctca tgttagacac caaatcccat atcgaaaccc cggagggggt
     4801 gcgcctgccc atcagcatcg ccagcatctg cccgcgcgcc tatgcctatg ggattgacct
     4861 gtttatccgc ctgatcgcgc tggcggcggt gtcttcggtg ttgggcgttc taggcaaact
     4921 cggcggcggc ttgatgctgt tgatcatgtt tagcctggag tggttttacc cggtggtgtt
     4981 tgacgtcttt aaccagggcg ccacaccggg taaacgcatc atgcagctgc aagtcgtgca
     5041 cgatgatggc agcccagtga gctttagcag ctccctgctg cgcaatttat tgatggtggt
     5101 ggattttctg ccggtgtttt attgcttggg cacactggta agtatttgcc acccgtttag
     5161 caaacgcctg ggcgatatag ccgcaggtac cctggtgatc caccaaagca ccgcatcacg
     5221 acacattcac cttgatgcag gcctgggcaa gcgagcccct tttgcgggcc tgagcctgcc
     5281 tgaacggcgc gccctggtga gttttgccga gcgctgcccg agcctcagcc aggcccgcca
     5341 agcagagctg gcgggcattt tggcgcccct gctgccggcg gactgcgccg aacaagccgt
     5401 gctggcactg caacagatgg ccaaccagat tgcaggcgcg agcatacctg cagacaagga
     5461 gccacagcca tgaaacaagc ggactttgaa cggcgcggcg agcccctttg gcgggccttt
     5521 gccgaagagc ttagcgtcct tgaagccagc gctcgcgccg tgccctcccc ccaatttgcc
     5581 caccattacc ggcagatttg ccaggcccac gccctgtgcg agcgccgcca gtacactgcc
     5641 caattggaga tgcaactagc ggatttagtg gtgcgcggcc agcgctacct ctaccggcgg
     5701 cccacgcgct ttgtgcaggc gttttggcgc tttgtgatca gcggtttccc ctcagctgtg
     5761 cggcgggaag cgcgcctgtt tgccctggtg agtgcactgt tttacctgcc tgccctgggc
     5821 ctcgcctggg ccctgagctt ccagccggat cttatctaca gcctgatgcc accccaggcg
     5881 gtgcagcacc tggagaacat gtaccagccg gacaatatca ccctggggga ggcccgcgac
     5941 tcaggcacca attgggagat gttcggcttt tacatccgca acaacatagg cgtggccttt
     6001 cgcacctgcg ccagcgggct cttattgggg gttggcagcc tcctgctgtt gctctacaac
     6061 ggcctgatgt tggggggcgc ggcggcccat ttgacccatt tgggttatca gcagacgttt
     6121 tttacttttg tcataggcca cggcagcttt gagctcaccg ctattgtgat tgccgctgtg
     6181 gcgggtctta aattgggcct cgccctggtg gcccccggcc gcctgccccg cctgcaggcg
     6241 ctgcgcctgg cgggggcgca ggccattgtg ctggccatgg gggcggggtt aatgctggta
     6301 gtggcggctt ttattgaggc cttttggtcg tcgaataacc tgctcccccc cggccttaaa
     6361 tatgcggtgg gcgccctcgg ctgggtatgt gtggctgcat acctctggtt tgcggggcgc
     6421 cgccgtggag cttgagcgcc gcgccattga aattcgccct cgcagcgcct gggaggcctg
     6481 cgatttgggc gtccagatgg gccgccactg gtggtggccc atggtcaaaa tctggctggc
     6541 cctggcggca ccttggctgt tgctcagctg gttgctaccg gcgcgcttcg ccttctggcc
     6601 ggccttttta tactggctgg gcaaacctct gtgggagcgg tttttactct actttctgag
     6661 ccaggcgatt tttaccagta ttcccagcac agcccaggta ctcaaaaacg ccccgctact
     6721 cttggccaaa gactggctgg caagcctgct ctggcgccgc ttaagcctat ggcgggccta
     6781 caactgcgcc ctcactgttc tcgagggaca aaccggcaag gcgcggcagc agcggcttaa
     6841 gcatttggcg ggcgataacg ccgccactgc atctaaggtg gccctgttgt tggcgcatat
     6901 ggaaggcttt ttgttgttat cccttttgat cctggcttat accctagcgc cgcgggatct
     6961 ggattggggc tttgcgcagc tgcacgagtg gctggcaagc gacacctttg ccgcaggtta
     7021 taacagcctg tacatcttgg tgagcaccct ggtagcgccg ttttttgtcg ccaccggttt
     7081 cgccctgtat ttaaaccggc gcatttggct ggaagcctgg gacatagaca tcagctttag
     7141 aaccctcgcc aagcgcctaa gccctgcgct cctactcatc ctgttgggca gctgcctgtg
     7201 ggcgcctggt ttggcaccgt cggcacaatc gcaggcagca caggcacaat cgcaggcagc
     7261 tcagccgcag acaaaagacc aacaaaccag tcagatcagc caggcgctag agcgtttagc
     7321 caaccccgcc agcttattta gcgagctgca acccgccccg gaacttatcg cccattaccc
     7381 cgccgagcag gcaagagttg ccgctgtgct ggcgggagaa gacttccacc gcaaggaaac
     7441 ccgcctggta caccactggc gctgggactg ggagagcaca cctgcgcaag acactgaccg
     7501 ggcccgctgg gagcaggtga ttgccatcac cgcccgcacc ctggaagtgc tgctgtgggc
     7561 gctggcattg gcccttat
//
LOCUS       DAOQGS010000153         7547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4694_length_7547_cov_5.475707, whole genome shotgun sequence.
ACCESSION   DAOQGS010000153 DAOQGS010000000
VERSION     DAOQGS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4694_length_7547_cov_5.475707"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2385
                     /locus_tag="PKZ52_07530"
     CDS             <1..2385
                     /locus_tag="PKZ52_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNG59663.1"
                     /translation="GTSATANSAYQKAKTALTNARTEPLTCKQIDAFGGEGGMYPDND
                     VYSIYRNPKSTRDNCVTDKNNTQDDEYAALVENYLAALKLGITADATALASLITSSPR
                     FNEASSYWWGAVAMEANLSLLTNQSLHTLNLDTLKANILQKADEIRANQALGYPGVTW
                     NPNSTQWNNGDQDTADNNVRWGSHRNALNDARILMAAAEIQKAKNLIPSAAAYARSAI
                     QVLDHMAGINAMNLAMFTATGYSSIFENAVERTHDGADSSTAWPGKLVLGPNNWTNAD
                     DPDMPAFNSKPGLKMFAPTGTGWSSREISIDANAALVAVAYYATEVAPALLALDPLGS
                     TGTTPNPPAAPSNLTAQATSSSAITLNWTDNAGNNNDAEQGFYVYQNTTNSKPVSALT
                     TLAANATSFIASGLNASTPYYFWVEAFNGQGVSSAITATATTSAGTTTPTNLISNGNF
                     ANGTTGWTCTINSGAATCSVVSGQYFVNITNGGTASWNIQPNQSNITLTSGKSYVFAF
                     DARATANRNAEIKIERAASPYEDFSKIGSGQALTTTMKRFVYPFTMTTSLTNARMVMN
                     VGNNVEDIYIDNIYLVEGTQDPCGGVVGCSGSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSGTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGT
                     AAKNIVVRMRGVAGTESVSLLINGVSLKTWTLTTSMADYAFSTTNSGAIRVNFTNDAT
                     GRDVQVDYITVNGQTRQAEQQTENTGAYGNNRCGGGAPSEWLHCSGYIGFGNTP"
     gene            complement(2843..3769)
                     /gene="nhaR"
                     /locus_tag="PKZ52_07535"
     CDS             complement(2843..3769)
                     /gene="nhaR"
                     /locus_tag="PKZ52_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HNG59664.1"
                     /translation="MNNLNYKHLRYFWMVARTGSIAKAAEQLHLTPQSISGQLTEFAS
                     VLGVELFRRAGRNLEITDAGRHVLTYAEEIFSLGDELLGAIHNQREKKSVPFVVGIAD
                     SVSKSVAYRLLEPALFLPEPIRLICREGRLVALLAELSVHRLDMIIADKPMPTNLNVR
                     GFTHLLGESGLTVFGAKALVKTLRGNFPKCLDDAPFLLPGDDTATRAKLEHWFEKHNV
                     HPRITGEFDDSALMKAFGQAGAGLFVGPSTISAHIEEQYDVKAIGQIKEVTEQLYVIS
                     TERQLTHPAIVAVSEKGRSDVFGARGTTKRTN"
     gene            3899..4261
                     /locus_tag="PKZ52_07540"
     CDS             3899..4261
                     /locus_tag="PKZ52_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNG59665.1"
                     /translation="MRIDIHTGKFSLTDSLRSHVERRADYALGWAQQSLQQVIVRLDD
                     LNGPKGGLDKLCRIQIPLVGGAVVVIAEVQADLYTAVDKAMERANQSLTRKLERKRKF
                     QFRRFTAPLMDVPQSFTS"
     gene            4291..5172
                     /gene="htpX"
                     /locus_tag="PKZ52_07545"
     CDS             4291..5172
                     /gene="htpX"
                     /locus_tag="PKZ52_07545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNG59666.1"
                     /translation="MKRIVLFLATNLAIVLVLSLSMRLLGAEPFLNANGLNLNALLVF
                     AAVMGFGGAFISLAISKWMAKKSMGVRVIETPATHQEAWLLATVQHYAKHSGIGMPEV
                     GIFDSPEVNAFATGMNKNKALVAVSSGLLAQMTQQEAEAVIGHEIAHIANGDMVTLAL
                     IQGVVNTFVIFIARIIGHLVDKIIFRTEQGTGPAFFISMIIAELILGVLASIIVMWFS
                     RQREFRADLGGAQLAGRANMIAALERLNSLHPAPLPDRLAAFGIAGGGMDGIKRLFMT
                     HPPLSERIEALRNLSRA"
     gene            5174..6163
                     /locus_tag="PKZ52_07550"
     CDS             5174..6163
                     /locus_tag="PKZ52_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNG59667.1"
                     /translation="MNDAISIGSPLLWAGFLSFVFIMLALDLFVFGAGKAHKVSVKEA
                     AGWSIGWFAMAIAFNAGLWWHLTGTLGSEIADQKALEFFTGYLIEKSLSVDNVFIFLL
                     IFSAFAVPPEYQRRVLIYGVLGAIVMRIIMILLGSWVVQEFKWILYIFGAFLLFTGIK
                     MLWASDSAQDMNENWLVKWARKHIRITPQLHGEKFTVRQEGVLYFTPLFLVLILVEVS
                     DLVFAVDSIPAIFAITTDPFIVATSNIFAILGLRALYFLLADMAERFHLLKYGLAVVL
                     AFIGTKLLITPWFHMPIALSLGFVVVVISLSIVASLWVTRKQANAKTAPSHQA"
     gene            complement(6446..>7547)
                     /locus_tag="PKZ52_07555"
     CDS             complement(6446..>7547)
                     /locus_tag="PKZ52_07555"
                     /inference="COORDINATES: protein motif:HMM:NF023010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HNG59668.1"
                     /translation="SSSSSSSSTSSSSSSSSGRTSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSGIANDALILVPDASWACGAPEGIPSPALGTLVMNATLQIGGIHEVGETQYGQR
                     RLLDVTGGNFTGPRIQGTFLTGGLELELILANDALELEQIDILRSQDGVPIYVRTCGF
                     AQAGANQSRFIADFEVANSSSYAWLNKGKYAGTRTVNERTGTVELAIYDISQVSSTGT
                     KVQLKDPTGVPNQPWECATTTGSKGSSVFTETVTLGSSISVGASKRGTRNIIPITGGN
                     VSGRFKGTVLPGGADYQLIGSSTKLDARYTLASSDGEYVIVRNCGAFGALIPQFEARK
                     NGPYAFLNDNKYLSSDPGSASGGVSITFYERK"
BASE COUNT         1916 a         1849 c         1847 g         1935 t
ORIGIN      
        1 ggcaccagcg ccaccgccaa tagcgcctac caaaaagcca aaacggcgct gactaatgcc
       61 cgcacggaac ctttgacctg caaacaaatt gatgccttcg gtggtgaagg cggtatgtac
      121 ccggataacg atgtgtacag tatttaccgc aacccaaaat ccacccggga taattgtgtt
      181 accgataaaa ataataccca ggacgatgaa tacgcagcct tagtggagaa ttatctggcg
      241 gcgttaaaat taggcattac ggcggatgcc actgcactgg caagtctgat taccagtagc
      301 ccaaggttta atgaagcatc ttcctactgg tggggtgcag tggctatgga ggcaaactta
      361 tccctattaa ccaatcaaag tttgcacacg ctgaatttag atacgttaaa agcgaatatt
      421 ctgcaaaaag ccgatgagat tcgcgcgaac caagccttgg gttatccagg cgtaacctgg
      481 aaccccaatt ccacccagtg gaataatggc gatcaggata cggctgataa taatgtgcgc
      541 tggggttccc accgcaatgc attaaatgat gcgcgtattt taatggcggc ggcggaaatt
      601 caaaaagcca aaaatctcat tccctctgca gctgcctatg cccgcagtgc aattcaggtg
      661 ttagaccata tggcaggcat taatgccatg aacctcgcca tgtttaccgc caccggttat
      721 tccagcattt ttgaaaacgc tgtggagcgc acccacgatg gcgctgatag cagcaccgct
      781 tggccgggta aattagtact cgggccgaat aactggacca atgcggatga cccggatatg
      841 cccgcattta attccaaacc cggtttaaaa atgtttgctc ccaccggcac cggttggtca
      901 tcgcgggaaa tttccatcga cgccaatgcc gcattagtgg ccgtagccta ttacgccacc
      961 gaggttgcgc ctgctctttt ggcgttagat cctttgggca gcactggcac tacgccaaat
     1021 cctccggctg cgccgagcaa tttaactgct caggccacaa gctcatcggc catcacatta
     1081 aactggacgg ataatgccgg taataataac gatgcggagc agggttttta tgtgtatcaa
     1141 aacaccacca acagcaaacc tgtaagcgcg ttaacgacgc ttgcagccaa cgctacctca
     1201 tttattgcca gcggattaaa tgccagtacg ccttattatt tctgggtaga agcatttaat
     1261 ggccagggtg tttccagcgc aattaccgcc accgctacca ccagtgcagg cactaccacg
     1321 ccaactaatt taattagcaa tggcaatttc gccaacggca ccaccggctg gacctgcacc
     1381 atcaatagcg gcgcggcaac ctgttccgtg gtaagcgggc aatattttgt aaatattact
     1441 aatggcggca ccgctagttg gaatattcaa cccaaccaaa gcaatattac gcttaccagc
     1501 ggtaaatcct atgtatttgc gtttgatgcc cgcgccacgg ccaatcgcaa tgcagaaatt
     1561 aaaattgaac gagcagcatc gccctatgaa gatttttcta aaatcggttc cggccaagcg
     1621 ctcaccacca ccatgaaacg ctttgtttat cccttcacta tgaccacttc acttaccaac
     1681 gcacgtatgg tgatgaatgt gggcaataat gtagaggata tttatatcga caatatttat
     1741 ttggtggagg gcacccaaga cccttgtggc ggcgttgttg gctgtagtgg ttcaagctcc
     1801 tcaagtagct cttcgagcag ttcatctagc tcgtcaagtt cttcttccag tagcagctct
     1861 tcgtcctcca gcagcagttc atccagcagt tcgtcaggaa cctcatcgag cagttcctct
     1921 agttcatcca gcagctcgtc gagtagctca tcttcgagca gttcatcttc aagtagctct
     1981 agttcatcat cgagcagttc ttcatccagt agttcttcaa gcagctcgtc atccagctcc
     2041 ggcactgcgg caaaaaatat tgtagtgcgc atgcgcggtg tggcaggtac tgagtctgtg
     2101 tcgttattaa ttaatggtgt gagcctaaaa acctggaccc tgaccaccag catggccgat
     2161 tatgcctttt ccactaccaa tagcggcgcc attcgggtta atttcaccaa tgacgcaact
     2221 ggccgggatg tacaagtgga ttatattacg gtgaacgggc aaacccgcca ggcggagcaa
     2281 caaacggaaa ataccggtgc atacggcaat aaccgctgcg gtggcggcgc gccgtcggag
     2341 tggttgcatt gcagtggtta tataggtttt ggcaacacgc cctaaataga ggccaaacgg
     2401 atttaatagc agtagatcag ctttaaggtt gtagccgttt agcccaagat ttgcctaatt
     2461 agcgtcttgg gcttttttgt gccttttaat taaggaacct cttaaaaata taggcggcga
     2521 agcgcaggct aagcgatttt gccaaaaaaa aagcggaatt tactaccgag ccacctgcaa
     2581 tgaatataca tcgtttcaga ccgccaaaaa gaaggacctt tttgacgagc ctacaaggat
     2641 gtatttacgg cgtgtctgaa atgatgtgta ttcattgctt ccacacagcc agtacacgca
     2701 gtgaatgagt attttttgca cattcaaacg gcgctagagc tagcaatgta gtatttcaac
     2761 aggctgttag agatttctta agaaattctg cgcaacagta ttgagctcat ttaagccacc
     2821 agaaaaaacc aagaaaagtt tattagttcg tgcgtttagt tgtaccgcgc gcaccgaata
     2881 catcgctgcg gcctttttcg ctcaccgcca caatcgccgg atgggttaat tgccgctccg
     2941 tgctaattac atataattgc tcggtaactt cttttatttg ccctatggcc ttaacgtcgt
     3001 attgctcttc aatatgggca ctgatagtgg aggggccgac aaataaaccc gcaccggcct
     3061 ggccaaaggc tttcattaat gcgctatcgt caaattcacc ggtaattctc gggtgcacat
     3121 tgtgtttctc aaaccaatgc tcaagcttgg cgcgggtggc ggtgtcgtcg cccggcaata
     3181 aaaaaggtgc atcatccagg catttaggga aattgccgcg cagagttttt accagcgctt
     3241 tagcgccaaa tacggttaaa ccgctctccc ccaataaatg agtaaacccg cgcacattga
     3301 gattcgtcgg catgggttta tcggcaataa tcatatccaa ccgatgtacc gacaattccg
     3361 ccaacagcgc taccaagcgc ccctctcgac aaattaaacg aatcggttcg ggcaaaaata
     3421 aggctggctc caataaacgg taggctacgg atttggacac cgagtcggca atacccacca
     3481 caaagggcac ggattttttc tcccgctggt tgtgaatagc gccgagtaat tcatcgccca
     3541 aggaaaatat ctcctcagca taggtgagca cgtggcggcc ggcatcggta atttctaaat
     3601 tgcggccggc gcggcgaaat aattccacac ccaataccga agcaaattcg gtgagctgcc
     3661 cgctgatgga ttggggcgtt aaatgcagct gttcggcagc cttagcgatg ctgccggtgc
     3721 gcgccaccat ccaaaaataa cgcagatgtt tgtaattgag gttgttcata ggcccccctg
     3781 tagtaatcga aaatttcgat gcttccatca attatattcg atttgctgga cgcattctaa
     3841 ttcactagca tcaattgcgt ggtatccctt tattcgcaac agccaagagg aaattcctat
     3901 gcgtatcgac atacacaccg gcaagttttc attaactgac agcctgcgca gccatgtgga
     3961 gcgccgcgct gattacgccc tcggctgggc ccagcaaagc ctgcagcagg tcattgtacg
     4021 tttagatgac ttaaacggcc caaaaggcgg cctggataaa ctctgccgga tacaaatccc
     4081 tttagtgggc ggtgcggtgg tagtgattgc agaagtgcaa gccgatttat acaccgccgt
     4141 agacaaagcc atggagaggg ccaaccagag tttaacgcgc aagttagagc gcaaacgtaa
     4201 atttcaattt agacgcttta cggcgcccct aatggacgtg ccgcaaagct ttacatcgta
     4261 atttttaatg cttgctgagg agacatagat atgaaacgta tagtgttatt tcttgccacc
     4321 aacctggcca ttgtattagt gctctcacta tccatgcgct tactgggcgc agagcctttt
     4381 ctcaatgcca atggcctgaa ccttaacgct ttattagtat ttgccgccgt aatgggtttt
     4441 ggcggcgcgt ttatttcctt agcaatatcc aaatggatgg ccaaaaaatc catgggtgtg
     4501 cgggtgattg aaacacccgc tacccatcag gaggcttggc tgctggcaac agtacaacac
     4561 tacgccaaac acagcggcat tggcatgccg gaggtgggga tttttgattc gccggaggta
     4621 aatgcctttg ccaccggcat gaataaaaat aaggcgttgg ttgctgtgtc gtcgggcctg
     4681 ttagcacaga tgacacagca agaagccgaa gcggtaatcg gccatgaaat cgcccacatc
     4741 gccaatggcg atatggtaac cctggcgtta attcaaggag tggtgaatac ctttgtcatt
     4801 tttattgcac gcattattgg ccacctggtg gataaaatta ttttccgcac tgaacagggc
     4861 actgggcctg cgttttttat cagcatgata attgccgaat taattctcgg tgtgctcgca
     4921 tccattattg ttatgtggtt ctctcgccag cgggaatttc gcgccgattt aggcggtgcg
     4981 caactggcag ggcgcgcgaa tatgattgcc gcgctggagc gtttaaattc cttacaccct
     5041 gctcctctgc ctgatcgttt agcggctttt ggtattgcag gtggcggcat ggatggcatt
     5101 aaacgcctat ttatgactca cccgccgttg agcgaacgca tcgaagcgct gcgcaaccta
     5161 tcgagggcat aacatgaacg acgcaattag tattggcagc ccactgttgt gggctggttt
     5221 tctcagtttt gtttttatca tgctcgcgct ggatctattt gtgtttggcg caggcaaggc
     5281 ccacaaggtg agtgttaaag aagccgctgg ctggtctatt gggtggtttg ccatggccat
     5341 cgcgtttaat gccggtttgt ggtggcacct aacgggtaca ctgggcagtg aaattgccga
     5401 ccaaaaggca ctggaatttt tcaccggcta tttaattgaa aaatccctat cggtggataa
     5461 tgtatttatc tttttgttaa ttttctcagc ctttgcagta ccgcctgaat accagcgacg
     5521 ggtattaatt tatggtgtgc tcggcgccat agtgatgcgc atcataatga ttctactggg
     5581 cagctgggtg gtacaagaat ttaaatggat actgtatata ttcggagcct tcctgttatt
     5641 tacgggcatt aaaatgctgt gggcgtcaga ttcagcacag gatatgaacg aaaactggct
     5701 ggtaaaatgg gcgcgcaaac atatacggat caccccacaa ttacacggtg aaaaattcac
     5761 cgtgcgccaa gagggtgtgc tgtattttac gccgctgttt ttagtgctga tattagttga
     5821 ggtgtcagat ttggtattcg ctgtagattc cattccggcc atttttgcca tcacaactga
     5881 cccgtttatc gtcgccacat ctaatatttt cgccatcttg ggcttgcgtg cgctgtactt
     5941 tttactggcg gatatggccg agcgctttca cctgctcaaa tacgggcttg ccgtggtgct
     6001 ggcatttatc ggcaccaaac tattaattac cccctggttc catatgccga ttgcattgtc
     6061 tttaggtttt gttgttgtag taattagttt aagcatagtg gcaagcctgt gggtcacccg
     6121 taaacaggct aatgctaaaa cagcgcccag tcatcaggcc taatcggctg ttgcacagac
     6181 ggaaaggttg gcaagagagc aacaagtgtt gaaagaaaca acccatcttg ctcccggttt
     6241 ggataaagcc gggggctttt tttatgggcg ataaaaaaca cgagtatttt ttggcagcgg
     6301 gatttttatg ccgggttagt tttttatccc ggctaaaacg ctatgcactt atttagagaa
     6361 tttcctaact cgcatacagc agtaggggcg cccccttgtg ggcgcccgtc aatcctgatt
     6421 aattttaccg ccagattagt actgtttatt tgcgctcgta aaaggtaatt gaaacaccgc
     6481 cactggctga gcctgggtct gaacttagat atttattatc atttaaaaac gcatagggac
     6541 catttttgcg cgcttcaaac tgtggaatta aggcaccgaa ggcgccgcaa ttgcgcacta
     6601 tgacatattc gccatcactg ctggctaggg tataacgagc atcgagtttg gtactcgagc
     6661 caattaattg atagtccgcc ccaccgggca acaccgtgcc tttaaaacgg ccgctgacgt
     6721 taccgccggt aatggggata atattgcggg taccgcgttt actggcgcct accgaaattg
     6781 acgagcctag ggtgaccgtt tcggtaaata cactagagcc cttgctgccc gtagtagtgg
     6841 cgcattccca cggttgattg ggcactccgg tgggatcttt taattgcacc ttggtgccgg
     6901 ttgaactcac ttgagaaata tcataaatgg ctaattctac agttcccgtg cgctcattta
     6961 ccgtgcgagt acctgcgtat ttgcccttat ttaaccaggc gtaactgctg ctattggcca
     7021 cttcaaaatc ggcgataaac cgcgattggt ttgcaccggc ttgagcaaag ccacaggtgc
     7081 gcacataaat aggcacgcca tcttggctgc gcaaaatatc tatttgctcc agctccagcg
     7141 cgtcattggc caaaattaat tccagctcta aaccaccggt taaaaacgtg ccttgaatgc
     7201 gcgggccggt aaaattaccg ccggttacat ccaataagcg gcgctggcca tattgagttt
     7261 cacccacctc gtgaatcccg ccaatttgta aggtggcgtt catcactaag gtgcccagtg
     7321 caggcgatgg aattccctct ggcgcaccac aggcccagga cgcatcgggc actaaaatga
     7381 gcgcatcgtt ggcgataccc gatgaagagc ttgaagagct ggacgatgag ctagaggatg
     7441 aacttgagga cgatgatgag ctggaactgg atgagcttga cgaactggtg cggccagagg
     7501 aagagctgga ggaggagctt gtagaagaac ttgaagagga gctgctt
//
LOCUS       DAOQGS010000154         7501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4750_length_7501_cov_4.858045, whole genome shotgun sequence.
ACCESSION   DAOQGS010000154 DAOQGS010000000
VERSION     DAOQGS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4750_length_7501_cov_4.858045"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1898
                     /gene="flgL"
                     /locus_tag="PKZ52_07560"
     CDS             303..1898
                     /gene="flgL"
                     /locus_tag="PKZ52_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNG59669.1"
                     /translation="MRISSLQIFDIANRNMSDLSGAITKTQEQISSGKRVLTPADDPV
                     ASTKILQLNEELANVKQYSANINAARNSLSLTETSLNGVTDLIHRIQELAISAGNTAT
                     LSKNEYAAYASEVDSRLQELMNLVNTKNANGDYIFAGFKSRQQPFTGDALSGFRYNGD
                     EGQQKIKVANNTFVPATDSGKTAFVDVPSSTNTINTYVSPSNKSDPPVKISVGRVVDQ
                     TLYDKFYPEDIVITFNADGNVVPQGKNFTATEKSTGRILLSNQPYTPGQEITVAGVAV
                     KISGNPVSGVAPVPATQAFGSALPTGPFDFSTSPQTLQIRAGGITQTLVLDANITNTA
                     DLAANLNDVANGNAARLASLGIRVDAQGFHMDQGINFSISGGSAAADTVTGLTTLAGS
                     QSTDGVLAIPGDRAFVDSSNRQDILTTLARFSETLKAYDGSTASRQAITGVVASTLDN
                     LNFAQTSVLNVVSTIGARVNTLDSTESQHLDTKVVGEKVLSDLSHLDYAEAATRLSQQ
                     SMVLEATQQSFIRVSQLTLFSKL"
     gene            complement(1936..3204)
                     /gene="glyA"
                     /locus_tag="PKZ52_07565"
     CDS             complement(1936..3204)
                     /gene="glyA"
                     /locus_tag="PKZ52_07565"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNG59670.1"
                     /translation="MFNPNETLAHFDPEIWASIQNEGHRQEEHIELIASENYTSPQVM
                     AAQGTKLTNKYAEGYPSKRYYGGCEYVDTAEALAIERAKTLFGADYANVQPHSGSQAN
                     AAVYLALLNPGDTILGMSLAHGGHLTHGAKVSASGKLYNSVQYGLNPETGLVDYEEVA
                     RLAREHKPKIVVAGFSAYSQIMDWQKFRAIADEVGAYLLVDMAHVAGLVAAGLYPSPV
                     AIADVTTSTTHKTLRGPRGGLILAKKNEEIEKKLNSAVFPGFQGGPLMHVIAAKAVSF
                     KEAMSPDFKTYQAQVIKNAKAMAKTFIERGLKIVSGGTENHLMLVDLIGKSYTGKDAD
                     EALGNANITVNKNAVPNDPRSPFVTSGLRVGTPAVTTRGFKEAECIDLANWMVDVLDS
                     LENGTSEATIAAVKDKVLTLCKKFPVYAAE"
     gene            3617..6307
                     /locus_tag="PKZ52_07570"
     CDS             3617..6307
                     /locus_tag="PKZ52_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG59671.1"
                     /translation="MSHRPFSATAAHLHAVYSALMQEQRLWGEDYLEGLQLISQTSAE
                     ALGAHICSVWCYSESRLDLRCQALFSDGQQVQPHLGHLLKRADHPDFCAALDDYRVVA
                     AADALNDSRLASFYAQLQQNSIGSLLDAVVRTEGQVGGFIRWERRGQGQAWLPDEEVF
                     ACSIADLLGQFFVFHKLRETEERYLKIFEAAGDAIAVLRGNRIVDCNKATLDMFRARR
                     EDLTQVPLRAFWAEFQPDGRRSADIEELTFGPARHGRPQQLIWRHKRFDGVEFDTEMG
                     ITPLMLRGRLHHIVCLHDITHRQRAENQIRELNTLQQAIFDGANYSIIATDDKGIIHT
                     FNRAAERMLGYSAREIIGKSTPARFHAENELRRRAQEATEELARPVVSGFETLVARVL
                     EGAVDEREWTYVRKDGSTLPILLSVTALRNNLGEVTGYLCIASDITERIRANAELLHS
                     KREIERNANHDLLTGLPNRSRLHDVARAAIRNAQERGQKLALMLLDLDRFKEVNDTLG
                     HAVGDQLLKQVAQRLQALLISYGAYLYRLGGDEFAILVTRIQDKDATMRLANTVKTSL
                     RQPIQIEGITLELGGSIGVALFPDHGDSSHGLLRCADVAMYKAKQQSSGALFYEPQFD
                     FHSPRRLTLLAELGAAIRNNDLILHYQPRVDLASGRCIGCEALVRWQHPTLGLIPPNE
                     FIPLAESGDLIQPLGLWVLETAMAQARQWENSGLDLIVSINLSARNLMENAFPQCIEQ
                     TLSAHGLPAERLEIEITESTLISDPERTLMVIHRINNMGVRFAIDDFGTGYSSLAYLK
                     RLPIQTLKIDRSFIRDLLSDDQDAMIVRSTLDLAHNFGLSVVAEGVEDEATLQELKRL
                     HCEQVQGFLFSKPLPVAEFEQWLHQFAGSLPQPNLAPPLH"
     gene            6917..>7501
                     /locus_tag="PKZ52_07575"
     CDS             6917..>7501
                     /locus_tag="PKZ52_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG59672.1"
                     /translation="MLPINLFAFHEGKIKILHLTWFAFFITFIMWFSQASLLPFIKQA
                     FALTDGQVKALLILNVALTIPARILIGVLVDKFGPRLAYSLLLAVSACFCFGYALADS
                     FEMLALMRFLVGFVGAGFVIGIRLVSEWFPAKEVGFAEGIYGGWGNFGAAVASGVLPV
                     LAAFYGGDNGWRWAVATTGFIALFYSVVFYTFARN"
BASE COUNT         1635 a         1951 c         2027 g         1888 t
ORIGIN      
        1 tctatttagc cttcgctgcc ctaaaactgg cgaaaatgcg tggatttcct tgttttaggg
       61 cagctcacgg cggtttgcac cgccgttgcg gcatccatgc cgccgggtta ataacagttt
      121 gaattattct gtatccacct ctttggcagt gcgattcctc ttaattttcc gcccgcgcgg
      181 aagagggatc atcggtgttg aatagttaca gcgctgcttt ggtgggttgt gcgtttgcgt
      241 gatgctatct ctggcacgga tactgcgtaa gataacaggt tgaactgtat tctaggtgtt
      301 ctatgcgtat ttcctcattg caaatatttg atattgccaa ccgcaatatg agtgacctaa
      361 gtggcgcgat cacaaaaacc caggagcaaa tttcctccgg taaacgggtg ttaacccctg
      421 cagatgaccc ggttgcctcc acaaaaattc tgcagctcaa tgaagaattg gccaacgtga
      481 agcagtattc tgccaatatt aatgctgccc gcaatagcct tagcttgact gaaacaagct
      541 taaacggggt aacggattta attcaccgca tccaagagtt agcgatttct gcaggcaata
      601 cggcaaccct gagtaaaaat gaatatgccg cctacgccag cgaggtggat tcgcggctgc
      661 aggagctgat gaatttggtg aataccaaaa atgccaatgg cgattatatt tttgcaggct
      721 ttaaaagccg ccaacagccc tttaccggcg atgctttgag cggctttcgt tacaatggcg
      781 atgaaggcca acagaaaatt aaagttgcca ataatacctt tgttcccgcc actgattcgg
      841 gtaaaaccgc ttttgtggat gtacccagta gcaccaatac aatcaatacc tatgtcagcc
      901 catcaaataa atccgatccg cctgtaaaaa taagcgtcgg gcgagtggtg gatcaaactc
      961 tttacgataa attttacccg gaagacatag tcattacctt taatgccgac ggcaatgttg
     1021 tgccgcaggg gaaaaacttt accgctacgg aaaaatccac cggccgaata ttgctcagca
     1081 accaacccta tacgcccggc caggaaatta cggttgcggg ggttgcggta aaaattagtg
     1141 gtaatcctgt ttcgggggtc gcgcctgtgc ctgcaacgca agcatttggt tcggccttgc
     1201 ccaccgggcc gtttgatttt tccaccagcc cacaaacgct gcagattcgc gcaggtggaa
     1261 ttacgcaaac tctggtgttg gatgccaata tcaccaatac cgccgatttg gcggcgaatt
     1321 taaatgatgt tgccaatggc aatgcggcgc gcttagcaag ccttggtatt cgcgtagacg
     1381 cccagggttt tcatatggat cagggaatta atttctcgat tagcggtggt tctgctgcgg
     1441 cggatacggt gacggggctt actacacttg cgggcagcca aagcacggat ggcgtgttag
     1501 ccataccggg tgaccgggct tttgtggatt cctccaatcg ccaggatatt ttgactaccc
     1561 tggcgcgttt tagcgaaacc cttaaagcct acgacggctc aactgcctcc cggcaggcta
     1621 ttaccggtgt ggtggcatcc accctggata atttaaattt tgcgcagacg agtgtattaa
     1681 atgtggtgtc taccattggt gcgcgggtga atactttgga ctccactgag tcacagcatc
     1741 tggatactaa agtagtaggc gagaaggttt tgagtgattt aagccatttg gattatgccg
     1801 aggcggcaac gcgtttatcg caacaatcta tggtgttgga agcgacccag cagtcgttta
     1861 ttcgcgtcag ccagctgacg ttattttcaa aactctaaaa gaatagggcg tccgtgccct
     1921 tgaatctgtg cagaattatt ccgccgcgta taccgggaat tttttgcaca aagttaaaac
     1981 tttgtctttc actgcggcaa tggtggcttc gctggtgccg ttttccaggc tgtctaaaac
     2041 atccaccatc caattggcaa gatcaatgca ctcagcttct ttaaagcccc gcgtggtgac
     2101 cgctggtgtg cctacgcgca aaccactggt aacaaagggt gagcgcggat cgttgggcac
     2161 ggcgtttttg ttaacagtga tattggcgtt gccaagggct tcgtcggcgt ctttacccgt
     2221 gtaggatttg ccgattaaat ccaccagcat taaatggttc tcggtgccgc cggagacaat
     2281 ttttaagccg cgctcaataa aggttttagc catggcttta gcatttttaa ttacttgcgc
     2341 ctggtaggtt ttaaagtctg ggctcatggc ttctttaaag ctcaccgctt tggcagcgat
     2401 cacatgcatt aatgggccgc cttgaaagcc tgggaacacg gcggaattta atttcttttc
     2461 aatttcttcg tttttcttcg ccagaattaa accaccgcgg ggcccgcgca gggttttatg
     2521 ggttgtggag gtggtcacat cggcaatggc cactggcgat gggtagaggc cagcggccac
     2581 caggcctgcc acgtgggcca tatccaccaa taaataagca cccacttcat cggcaatggc
     2641 gcgaaatttt tgccaatcca taatctgtga atacgcggag aaacccgcca ccactatttt
     2701 gggtttgtgt tcacgggcga ggcgggccac ttcttcatag tccaccaggc cagtttccgg
     2761 gttcagcccg tactgcacag aattgtagag tttgccggag gcgctcacct tggcgccgtg
     2821 ggtgaggtgg ccgccgtgag ctaggctcat gcctaaaatg gtatcgcctg ggtttaacag
     2881 ggccaggtaa acggcagcgt tggcttggga gccggagtgg ggttggacgt tggcgtagtc
     2941 ggcgccgaac agggttttgg cgcgctcgat ggcgagggct tcggcggtgt ccacatattc
     3001 acagccgccg taatagcgtt tgctggggta gccctcggcg tatttattgg tgagcttggt
     3061 gccttgggcg gccattactt gggggctggt gtagttttca gaggcgataa gctcgatgtg
     3121 ctcttcttgt cggtggcctt cgttctggat ggatgcccat atttcagggt caaagtgagc
     3181 cagggtttca ttggggttaa acataaagac ctcgagtctg ccggtggttt tgagaaaaag
     3241 cgcgcattgt aaccaaactt accccaggcc gacagcgctc tgcgcatttc ccggccagtc
     3301 ttaaaacctt ggtggttgag ctacgccgct gctcgccttt acgccacgat tgctaactct
     3361 ggctttagct agccccgtgg gccataggta tccggctaag cttgtactta ctcaggccac
     3421 ctctggggca agtaggccca aaatggctct gcggtgcggt tggcaaataa ccacaatccc
     3481 tagttggcgc cgggataatt gtgtttcttc ttctatactc ctctaagtcc gggcgaattt
     3541 gcctggcggt cttttagctt ggttttacgc gcttgctgca gtatcgattg atttatctaa
     3601 taagaattgt gcccccatga gccaccgccc gttcagcgcc acagctgccc atcttcacgc
     3661 cgtttacagt gctcttatgc aggagcaacg gctatggggg gaagattatc tagagggttt
     3721 gcagcttatt agccaaacca gcgccgaggc cttaggggcg cacatctgta gcgtatggtg
     3781 ttacagcgag tccaggctcg atctgcgctg ccaagcctta tttagcgatg ggcaacaggt
     3841 gcagccgcat ttagggcacc tgcttaagcg cgccgaccac ccagactttt gcgcggcgct
     3901 ggatgattac cgcgtcgtcg ctgcggcaga tgccctcaat gacagccgct tggccagctt
     3961 ttatgcacag ctgcaacaga attccatagg cagcctgttg gatgctgtgg tgcgtacgga
     4021 agggcaggtg ggtggcttta tccgctggga gcgcaggggc caaggccagg cgtggctgcc
     4081 ggatgaggag gtatttgcct gctcaattgc cgatttgctg ggccagtttt ttgtctttca
     4141 caaactgcgg gagaccgagg agcgctattt aaaaattttc gaagccgccg gtgatgccat
     4201 agcggttttg cgcggcaatc gcatagtgga ttgcaacaag gccactttgg atatgtttcg
     4261 cgcccgccgg gaggatttaa cccaggtgcc cttgcgcgcc ttttgggctg agttccagcc
     4321 ggatggacgc cgctccgccg atattgaaga gcttaccttt ggccccgccc gccacggcag
     4381 gccgcagcag ttaatctggc gccacaagcg ttttgacggg gtggagtttg atactgaaat
     4441 gggcattacc cctctcatgc tgcgcggccg cctgcaccac atcgtgtgtc tacacgacat
     4501 cactcatagg cagcgggcgg aaaatcaaat ccgcgagctc aataccctac agcaggccat
     4561 cttcgacggc gccaactaca gcatcatcgc caccgacgac aaaggcatta tccacacctt
     4621 taaccgcgcg gccgagcgca tgttgggcta cagcgcccgc gagatcatcg gcaaaagcac
     4681 accggcgcgc tttcacgccg agaatgaact gcgccgccgc gcccaggaag cgactgaaga
     4741 actcgcccgc ccggtggtca gcggctttga gaccttggtg gcgcgggtat tagagggcgc
     4801 tgtggacgag cgcgaatgga cctatgtgcg caaggacggc agcaccttgc cgattctgtt
     4861 atcggttacg gctctacgca ataacctagg cgaggtgacg gggtatttat gcatcgcctc
     4921 agatatcacc gagcgcattc gcgccaacgc tgaattactg cactccaaac gggaaattga
     4981 gcgcaacgcc aatcacgatt tattaaccgg cctgcccaat cgttcgcgcc tgcacgatgt
     5041 ggcccgcgcc gctatccgca acgcccagga gcgcggccaa aaactggccc tgatgctgct
     5101 ggatttagac cgctttaaag aggttaacga cacccttggc cacgccgtgg gggatcaact
     5161 gctcaagcaa gttgctcagc gcttacaggc gctgctaatc agctacggcg cttatttgta
     5221 ccgtttgggc ggcgatgagt tcgccatttt agtaacccga attcaagaca aagacgccac
     5281 catgcgcctg gccaacacgg tgaagaccag cctgcgccaa cccattcaaa ttgagggcat
     5341 aaccctggag ctgggcggca gtattggcgt ggcgctgttc cccgaccacg gcgatagcag
     5401 ccacggcttg ctgcgctgcg ccgacgtggc catgtataaa gccaaacagc agtcctccgg
     5461 cgccttgttt tatgagccgc agtttgattt tcacagccct cggcggctga ccctgttggc
     5521 ggagttgggc gccgctattc gcaacaacga tttgattctg cactaccagc cgcgagtgga
     5581 tttagccagc gggcgctgca tcggctgtga agccctggtg cgctggcagc acccaaccct
     5641 cggtttaatc ccccccaacg agtttatccc cctggccgaa tccggcgatt taattcagcc
     5701 cttggggctg tgggtattgg agaccgccat ggctcaggcg cgccagtggg agaacagtgg
     5761 gttggattta atcgtctcca ttaacctctc cgcccgcaac ctgatggaaa acgccttccc
     5821 ccagtgtatt gaacaaaccc tctcggccca tgggttgcct gcggagcggt tggaaattga
     5881 aattaccgaa agcacattaa tcagcgaccc tgagcgcacc ctgatggtta ttcaccgtat
     5941 caataatatg ggcgtgcgct ttgctatcga tgattttggc accggttatt cctctttggc
     6001 ctatttaaaa cgcctgccaa ttcaaaccct aaaaatcgac cgctcgttta tccgcgacct
     6061 gctcagcgac gatcaagacg ccatgatcgt gcgctccact ctggatttag cccataactt
     6121 tggcctgagt gtggtggcag aaggggtgga ggacgaggcc acgctgcagg agcttaagcg
     6181 cctgcactgc gaacaggtgc agggcttttt gtttagcaaa ccgctgccgg tggccgagtt
     6241 tgagcagtgg ttgcaccagt ttgcaggctc gctgccgcag ccaaatctcg ccccgccgct
     6301 gcattagttg ttctcgctca tgctcaatcc ctctggaggc ctcgctggcg ctcatggcca
     6361 atgcctggcc tgatggcata accgccacag gagggaaatt tggggcctgc tccctaagca
     6421 aattcttaac ctgatgttcc ccttggcgga gttggaattt catcgggaat taaatcgcta
     6481 tggcgccatg ggttagcgat tgcctgcttc ttaagctatg gatagggatt ttaggcctga
     6541 cgcccagttg aaattaaacc cgccaggcca ggcagggtga gcagagtgca aatagccgcg
     6601 aattcagact tactcagagc gcctccactg aaaaactccc ttttattggg gctatttaag
     6661 ggcacttaaa atgcgcttgg caccatcaat agaaattatt tccagtttag agtgaattaa
     6721 attggtgcct attggttgta tgtagggcaa aaatattgca ctaatcagtg ctttgatggc
     6781 tacaaagcga ggctatcggc acttattttg gcaatgttat ggtgcatata aaaaataaac
     6841 ccaataaaaa caaatacata aattgaatgg cctcgatatt gctaaagctt tgcaaacccc
     6901 actctgagtt tgctctatgc tgcccatcaa tctgtttgcc tttcatgaag gcaaaatcaa
     6961 aattctgcac ctcacctggt tcgcattttt tatcaccttc atcatgtggt ttagccaggc
     7021 gtctttgttg ccctttatta aacaagcctt cgccctcacc gatggccagg taaaagcgct
     7081 actgattttg aacgtggcgc ttaccattcc ggcccgtatc cttattggtg tgttggtgga
     7141 taaattcggc cccaggctcg cctatagcct tttgttggcc gtatccgcct gtttttgttt
     7201 tggctatgcc ctagctgata gttttgagat gctcgcccta atgcgctttt tggtggggtt
     7261 tgtgggcgcg gggtttgtga tcggcatccg cttggtatcc gagtggttcc cggccaaaga
     7321 agtgggtttt gccgaaggga tttacggcgg ctggggtaat tttggtgccg ccgtggcctc
     7381 cggtgtactg ccggtattag cggcttttta tggcggcgac aacggctggc gctgggcggt
     7441 ggccaccaca gggtttatcg ccttgtttta ttcggtagtg ttttacacct ttgcccgcaa
     7501 c
//
LOCUS       DAOQGS010000155         7497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4752_length_7497_cov_4.057108, whole genome shotgun sequence.
ACCESSION   DAOQGS010000155 DAOQGS010000000
VERSION     DAOQGS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_7497_cov_4.057108"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..1119)
                     /locus_tag="PKZ52_07580"
     CDS             complement(412..1119)
                     /locus_tag="PKZ52_07580"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNG59673.1"
                     /translation="MSRNTHTDDLNQQAFEFVIGTLRGTERQAFAQVITVNAEAARQV
                     HFWEEQLMALPEPPPIQPAQQTWHNIAARLSGETPVNSATSSLWWRLKNWLIPGALGS
                     ICTLLVVLIVYTGSAPSGSEYIAVLNDEQGRPALTAITRGDTANMRIQWQPLTIAKSK
                     NLQLWAISARDGQARSLAVFDTPSGEQLELDTPQLRLIKDADSLLLTEEDVGGSAIDE
                     PSSIVVAKGMCVRFAGR"
     gene            complement(1089..1664)
                     /locus_tag="PKZ52_07585"
     CDS             complement(1089..1664)
                     /locus_tag="PKZ52_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNG59674.1"
                     /translation="MNSRDDELIALIARCAIRDQAALREIFDRLAPYLNGVALRIVRS
                     EDASNDVLQEAFIQIWQNASSYRPHLSKPLTWLTSIVRYRALDRFAQDKSLREKFDPN
                     ISEEEFCAIADDNCPVRDASADQMRNQLAHCLDSLSTSIKQSVELAYIHGYSRDEIAG
                     QMNTNSNTVKSWLHRGAQRLKQCLETRIQTI"
     gene            complement(2198..2274)
                     /locus_tag="PKZ52_07590"
     tRNA            complement(2198..2274)
                     /locus_tag="PKZ52_07590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2238..2240),aa:Pro,seq:ggg)
     gene            complement(2388..2759)
                     /locus_tag="PKZ52_07595"
     CDS             complement(2388..2759)
                     /locus_tag="PKZ52_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNG59675.1"
                     /translation="MLEPSHNQELPEIPAKRYFSIGEASELCAVKPHVLRYWELEFPQ
                     IKPVKRRGNRRYYQRKDLEAIRTIRGLLYDRGFTVSGARQQLLFDASPKNTNEGDDVI
                     QEVLAELEQLLTELRQTANLP"
     gene            complement(2740..3045)
                     /gene="ihfA"
                     /locus_tag="PKZ52_07600"
     CDS             complement(2740..3045)
                     /gene="ihfA"
                     /locus_tag="PKZ52_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNG59676.1"
                     /translation="MSESLTKADLAERLYEELGFNKREAKDLVDAFFDTVRAALEGNE
                     SVKLSGFGNFDLRDKRQRPGRNPKTGEEIPITARRVVTFKPGQKLRERVEAYAGTES"
     gene            complement(3050..5419)
                     /gene="pheT"
                     /locus_tag="PKZ52_07605"
     CDS             complement(3050..5419)
                     /gene="pheT"
                     /locus_tag="PKZ52_07605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNG59677.1"
                     /translation="MKISESWLREWVNPPVSTEQLVHQVTMAGLEVDAITPVAGQFNG
                     VVVARIISAEPHPDAQKLRVCQVDAGGEPLQIVCGAANARAGIKVPLAQGGAPLPGGL
                     EIKKAKLRGVESFGMLCGQTELAAGDDDSGLWELAHDAPVGADLREYLQLSDQILELD
                     LTPNRSDCLSIRGIAREVGVLTQAPVKTFEQAAQPVASDHKKSVQVLAPAGCPRYLAR
                     IIDNIDIKTPSPLWLRAKLERAGLRSIDAVVDVTNFVLLELGQPLHAFDLAKLQGAIQ
                     VRMATAGEQLTLLNDQHISLREDCLVIADEAQPLALAGIMGGKASAVSATTQSILLES
                     AFFSPAVIAGRARSFGLHTDSSHRFERGVDYELQRIALECATELLLAIVGGSAGPIVE
                     AVATEQLPIKAPIRLSKQRLAQGLGLEIPSAEVVRMLTSLGLGLQEETAEHWLFTVPS
                     YRFDIAIEADLLEEVARIYGYARLPTRISAFAVEVANISDSQTEKATLKRHLVSRNFQ
                     EIITYSFIDPKLHETLFPTQPAVQLQNPISADMASMRTSLLPGLITTLKTNLNRQHTR
                     AQLFETGLVFAAEAGYPQQEKLAGLIYGPRHGLHWAHDKTDVDFYDVKGEVELLFALG
                     GRAIQVQAAADLPHMHPGQAAYVLVDGKTVGVLGALHPVVVKNLDLNKPAFVFEIDLA
                     ALRSGSLPQASGISRFPEVSRDLALVVDKNLAALSVQKVIEHAAGEGLKQVTLFDVYA
                     GQGIDLARKSLAFSLLFQHSSRTLTDEEIQASLDRVIQRVAEEFNATLR"
     gene            complement(5447..6460)
                     /gene="pheS"
                     /locus_tag="PKZ52_07610"
     CDS             complement(5447..6460)
                     /gene="pheS"
                     /locus_tag="PKZ52_07610"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNG59678.1"
                     /translation="MSELQSLIAEAKVHIAAAADLAALDQVRVDFLGKKGLITAQLKS
                     LGALSPEERPAAGAKINEAKDAINEALNERKTVLEQAQINAQLAAQALDISLPGRNNS
                     LGGLHPVSRTLMRIEAIFRAAGYQVAEGPEIEDDFHNFEALNIPAHHPARAMHDTFYV
                     DPTHVLRTHTSPVQIRTMESQRPPIRIICPGRVYRCDSDMTHTPMFHQVEGLVVDEQV
                     SFADLKGTMDQFLKAFFESDVAVRFRPSYFPFTEPSAEMDIQCTQCHGAGCRMCKHTG
                     WLEVGGCGMVHPEVFKACGLDPEAVSGFAFGMGVERLAMLRYGVNDLRLFFENDLNFL
                     RQF"
     gene            complement(6728..7087)
                     /gene="rplT"
                     /locus_tag="PKZ52_07615"
     CDS             complement(6728..7087)
                     /gene="rplT"
                     /locus_tag="PKZ52_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNG59679.1"
                     /translation="MARVKRGVEARRRHKKILKLAKGYYGARSRVFRVAKQAVIKAGQ
                     YAFRDRRVKKRNFRALWITRINAQSRAEGLSYSRLIAGLKKANITLDRRVLADLAIHD
                     KSAFAVIVSQAKSALAA"
     gene            complement(7126..7320)
                     /gene="rpmI"
                     /locus_tag="PKZ52_07620"
     CDS             complement(7126..7320)
                     /gene="rpmI"
                     /locus_tag="PKZ52_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNG59680.1"
                     /translation="MAKAKIHSGAAKRFKKTAAGFKRKHAFKSHILTKMTTKRKRQLR
                     GTSMVHDSDVSNVKRMLRLN"
     gene            complement(7381..7488)
                     /locus_tag="PKZ52_07625"
     CDS             complement(7381..7488)
                     /locus_tag="PKZ52_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59681.1"
                     /translation="MQRIEKDLEDMGTVEQHAKMEGRQLTMVIAPKKKK"
BASE COUNT         1781 a         1879 c         1926 g         1911 t
ORIGIN      
        1 gtctgtagtt gagtcaactg ctctagtcct tctgtgctca ctatgcctcg gttggcgcat
       61 tgtaattcca aaagaactgc cgcgtctttg atttgctggc ggctcatgtg ttcatcgata
      121 cagcgggcta attctggatc ttttataggc gcctctaata ccattaaagg taatgccatt
      181 atagggcagt aaaaaacaga tatcgcagcc cccaccacca ttttatgggc cagcaagttt
      241 tttagcgaga taaaatgtcc catggtgaag ctgtgcctag atgattcact cttagttaag
      301 ttgattacta attaattttt tggtgtaaag gcggcaagaa tacgcgcatt gatgatgcgc
      361 ttatttcttc cttattccca ccaaagtgag aatagggtaa gaaaatagca attaacgccc
      421 tgcaaatcgc acacacattc ctttggccac gacaattgag ctgggttcat caatagcaga
      481 gccacctaca tcttcttccg tgagtaatag gctgtcggcg tcttttatca ggcgcagttg
      541 cggcgtatcc agctccagct gctcaccact gggcgtgtca aaaacagcca aagaacgcgc
      601 ttgtccatct cgtgcagaga tggcccataa ttgcaaattt ttgcttttgg caattgtcaa
      661 cggctgccat tgtatgcgca tattagctgt gtcacctcgg gtgattgctg ttaatgcagg
      721 gcggccctgc tcatcgttta aaaccgcaat atattcgctg ccggatggtg cactacctgt
      781 atacacaata agcaccacca ataaggtaca aatactgccg agtgcacctg gaatcagcca
      841 attttttaaa cgccaccaga gcgagctggt ggcagaattt actggcgttt cgccacttaa
      901 gcgcgcggcg atattgtgcc aggtctgttg agcaggttgt atcgggggcg gctctggtag
      961 cgccattaat tgttcctccc aaaaatgtac ttggcgcgca gcctcagcgt tgactgtgat
     1021 tacttgggcg aatgcttggc gctcagtgcc gcgcaatgtg ccaatcacaa actcaaacgc
     1081 ttgctgattt aaatcgtctg tatgcgtgtt tctagacatt gttttaatct ctgtgcgccc
     1141 cggtgtaacc aggatttaac cgtattgctg ttggtattca tttgcccggc aatttcatcg
     1201 cgggaataac cgtgtatata agccagctcc acggattgtt taatgctggt gcttaggcta
     1261 tccaagcagt gcgcaagttg gttgcgcatt tgatcggctg aagcgtcgcg cacaggacag
     1321 ttatcatcag caatcgcgca aaattcttcc tcgcttatgt tggggtcgaa tttctcacgc
     1381 agtgatttat cctgagcgaa acgatccagc gcgcggtagc gtactatgct tgtgagccag
     1441 gtaaggggct tggataaatg ggggcggtaa ctgctggcgt tttgccaaat ttggataaat
     1501 gcttcctgca aaacatcgtt gcttgcatcc tcgctgcgca ctatacgcag ggccacgcca
     1561 tttaaataag gcgcaagccg atcaaatatt tccctcagcg cagcttgatc gcgaatcgca
     1621 cagcgcgcaa tcagtgcaat taattcatca tcccgtgagt tcataggtga gccatgtata
     1681 aacaattaat tgatcgcctt tagctgctac taaaggtaag tgccacagcc atccccgcaa
     1741 ttaaaatacc cctgtgattt tccattctgg ctgtattgtg cagatatacg ggatgaggac
     1801 gctaaaagtt gcaggtgcgc ctaaaatcct gttgataaga cggtttttgc gctgtgattg
     1861 tctagttcag gttggggtgc caacttcggc taaagattac gcgagtttgc aacaatgtat
     1921 aagtagatta acgcagcctg gcccgggtgt tttgtgccag taaggctatt tgaaatagac
     1981 ttaattaagg cctttattat aaaaagagga atgcggatgg attttattcg ctcaattgcc
     2041 tgcgggataa ataactagaa gcttgagccc aaaaatgctg gcattaaatc acgtgtctat
     2101 tgagtagcga tttgtaagat cggtagagct tgataattcg ccacggaaaa gaggttttgt
     2161 gtgcctaatc gacagagaaa tcgagaaggg gaaaaattgg tcggaacggc gggatttgaa
     2221 cccacgaccc ctacaccccc agtgtagtgc gctaccagac tgcgctacgc tccgaattcg
     2281 ttgaccactg ctaacccttt gtttttaaaa catttaaaga ttagcagtgc tgtaagaggc
     2341 gcacataata ccgaaaactc gtttatatgc aaggtttttt atgatgctta aggtaaattt
     2401 gccgtttggc gcaactcggt tagcagttgt tctaattcgg ctaacacctc ttgaatgaca
     2461 tcatcgcctt cgttggtatt tttagggctg gcgtcgaata gtaattgttg ccttgcgcca
     2521 ctcacagtaa atcctctgtc ataaagcagg ccccgtatgg tgcggatcgc ctcgagatcc
     2581 ttgcgttggt aatagcggcg gttgccacgg cgtttcaccg gtttgatttg gggaaattcc
     2641 agttcccagt aacgcaaaac gtgtggcttt accgcgcaca gctcgctggc ttcgccaata
     2701 ctgaaataac gcttggcggg aatttccggc aattcttgat tatgactcgg ttccagcata
     2761 ggcctctact cgctcgcgca acttttggcc cggcttaaac gtcactaccc ggcgggcggt
     2821 aatgggaatc tcttcgcctg ttttggggtt gcggcctggg cgctggcgct tgtctcttaa
     2881 gtcaaaattg ccaaagccgg agagcttgac gctctcattg ccttccaggg cggcgcgcac
     2941 tgtatcaaaa aacgcgtcca ccaggtcctt ggcttcccgt ttgttaaacc ccaattcttc
     3001 gtacaaacgc tccgccaaat cagcttttgt cagagattca gacatagact tacctcagag
     3061 ttgcattaaa ttcctctgcc acccgctgga taacccgatc caggctggct tgaatttcct
     3121 catctgtaag agtgcgcgag gaatgctgga atagcaagct aaacgccaaa cttttgcgtg
     3181 ctaaatcaat accctgccca gcatacacat caaacagtgt gacctgcttt aaaccttccc
     3241 cggccgcgtg ttcaatcact ttttgaacgc ttagggccgc taggttttta tctaccacaa
     3301 gtgctagatc tcgtgacact tcgggaaagc gagagatgcc gctcgcttgt ggcaggctgc
     3361 ccgagcgcaa agcggctagg tcaatttcaa atacaaaggc aggtttattt aaatctaaat
     3421 tcttcaccac aacaggatgc agcgcgccca gcacgcctac ggttttgcca tcgaccaata
     3481 cataggctgc ttggcctggg tgcatatggg gcagatcggc ggccgcctgg acttgaatgg
     3541 cgcgcccccc cagtgcaaac aacagctcga cttcaccttt tacatcgtaa aagtccacgt
     3601 cggttttatc gtgggcccag tgtaaaccgt ggcgtgggcc gtagattaaa ccggccagtt
     3661 tctcctgctg agggtaccca gcctcggcgg caaacaccaa gccggtctca aataactgag
     3721 cgcgggtgtg ctggcggttt aaattggttt taagtgttgt aatcaggccg ggcagtaaag
     3781 aagtgcgcat gctcgccata tcggcgctga tagggttttg tagctgcaca gctggttgag
     3841 tggggaagag cgtctcgtgc agtttaggat cgatgaagct gtaggtgatg atttcttgga
     3901 agttgcggct caccaggtgg cgcttgaggg tcgctttttc agtttggcta tcgctgatat
     3961 tggcaacttc tactgcaaaa gccgaaattc gcgttggcag acgggcatag ccgtaaatgc
     4021 gcgctacttc ctcgagtaaa tccgcctcaa ttgctatatc aaagcggtag ctcggcacag
     4081 taaacagcca atgctcggcg gtttcttcct gtaaccccag gcctagggac gttaacatcc
     4141 gcacaacttc cgcggaagga atttccagcc ctaagccctg ggccaggcgt tgtttgctca
     4201 ggcggatagg cgctttaata gggagttgct cggtggctac cgcttctact atagggccag
     4261 cactgccgcc tactatggcg agcaacaatt cggtagcgca ctctagggca atgcgttgta
     4321 attcatagtc cacccctcgc tcaaagcggt gggaggagtc ggtgtgtaaa ccaaaactgc
     4381 gcgctcgccc ggcgattacg gcggggctga aaaaggcact ctcgagcaaa attgattgag
     4441 tggttgcgga aacggccgag gctttgccac ccataatgcc tgctaaagcc agcggctggg
     4501 cctcatctgc aatcactaag cagtcttctc gcaagctaat gtgctgatcg ttcagaaggg
     4561 tcaactgctc gccagcggtt gccatgcgca cctgtatagc gccttgcagt ttcgccagat
     4621 caaaggcgtg caggggctgg ccaagttcca acaagacaaa attagtgaca tctaccaccg
     4681 catcaatgga gcgcaggcca gcgcgttcta gtttggcgcg cagccataaa gggctgggcg
     4741 ttttgatatc gatgttgtcg atgattctcg ccaggtacct agggcagcct gcaggtgcta
     4801 atacctgcac ggatttttta tgatcgctgg caactggctg agccgcctgc tcgaaggttt
     4861 taaccggtgc ttgagttaaa acaccaacct cccgtgctat cccgcggatg ctcaaacagt
     4921 cgctgcggtt gggagttaaa tccagctcta aaatttgatc tgataactgc agatactcac
     4981 gcaaatccgc gccaacaggg gcgtcgtgcg caagctccca caggccgctg tcatcgtcgc
     5041 cagcggccag ttctgtttgc ccgcacaaca tgccaaagga ttcaacgccg cgcagtttgg
     5101 cttttttaat ctctaaaccg ccgggcaatg gggcacctcc ctgagctaga ggaaccttaa
     5161 tacctgcccg cgcattggcg gcgccgcaga caatttgcag gggctcaccg ccggcatcta
     5221 cttggcaaac gcgtaatttc tgggcgtcag ggtggggttc ggcgctgatg atgcgcgcca
     5281 caaccacgcc gttaaattgc ccggccacag gggtgatggc gtccacttct aggcccgcca
     5341 tcgtgacttg gtgcaccagt tgctcagtag aaaccggtgg gttgacccac tcgcgcaacc
     5401 aggattcgct aattttcata ggtataaact gcttaagaag tcgatgttaa aactgtcgga
     5461 ggaagttcag atcattttca aagaagagcc gtaggtcgtt aaccccgtag cgcagcatgg
     5521 ccaagcgctc aacccccatg ccgaaggcaa aaccactcac ggcttctgga tctaggccgc
     5581 aggccttaaa aacttctggg tgcaccatgc cgcaaccacc cacttctaac cagccggtgt
     5641 gtttacacat gcgacagcct gcgccgtggc actgggtaca ttgaatatcc atttcggctg
     5701 agggttcggt gaaggggaaa taggatgggc gaaaacgtac cgctacgtca gactcaaaaa
     5761 acgccttgag gaattgatcc atagtgcctt ttagatcggc aaaactgacc tgctcatcca
     5821 ccaccaggcc ttccacctga tggaacatgg gcgtatgggt catatcggaa tcacagcgat
     5881 aaacacggcc tgggcagata atgcggatag gtggtcgctg tgattccata gtgcgaattt
     5941 gcaccggcga ggtatgggtg cgcagcacgt gggttggatc cacatagaag gtatcgtgca
     6001 tggcgcgggc agggtggtgg gcgggaatat tgagggcttc gaagttatga aaatcatctt
     6061 cgatctcggg cccttcagcg acttgataac ccgccgcgcg aaatattgcc tcgatgcgca
     6121 ttagtgtgcg gcttacaggg tgcaaaccac ccaggctgtt attgcggcct ggcaaactaa
     6181 tatccaacgc ttgggcagcc agttgggcgt taatctgtgc ctgctctaag actgttttgc
     6241 gctcgttgag cgcttcattg attgcgtctt tcgcctcgtt gatttttgcc ccggccgcag
     6301 ggcgctcttc cggcgatagt gcgcccaagg attttaactg cgccgtaatt aggccttttt
     6361 taccgagaaa atcgacgcgc acctgatcga gcgcggcaag atctgctgcg gcggcaatgt
     6421 gaactttagc ctcggcaatc agcgattgga gttctgacat gtggttatcc caaaaaacgc
     6481 taggttatat cgaacaataa aacatcggcc cagcaccagt gtgccgcaaa tagtttgctt
     6541 tgccgtgaca attattaagt ggtgaatatt ggattgggtg tgacggtgtt gatataccgc
     6601 tgttaataaa aaaggggttt agccaaggcc aacccctttt aatccgaact gctgaaaacc
     6661 taaagcgaac cctaattact cagaactcgc tatcgccccc aggctcggat gcgcaacacc
     6721 caaaggctta agcggctaag gcagacttag cttgtgacac aattactgca aaggcagatt
     6781 tatcgtgaat agccaggtca gccaatacgc ggcgatccag ggtgatgttg gctttcttca
     6841 aaccggcgat caagcggctg taggacaagc cttcagcgcg tgattgagcg ttgatacgag
     6901 tgatccaaag tgcacggaag ttgcgctttt taactcgacg gtcgcgaaag gcgtattgac
     6961 cagctttgat aaccgcttgc ttggcaacgc gaaatacgcg tgaccgagct ccatagtaac
     7021 cttttgccag ctttaagatt tttttgtggc gacgacgcgc ctcgactcca cgtttaacac
     7081 gagccatggt ttttcctcac gaaaattaaa tgaacaaaac gaaaattagt tgaggcgcaa
     7141 catgcgctta acattgctga catcagagtc gtgcaccatg gaggtaccac gcagctgacg
     7201 cttacgctta gtcgtcattt tggtgaggat gtggctttta aacgcgtgct tgcgcttgaa
     7261 accggcggct gttttcttga agcgcttggc ggcgccactg tgtattttag cttttgccat
     7321 tgttaaatct ccgatagcga gatgttgagt gtgaaagtag cgaggcagct aaaagcccga
     7381 ctactttttc tttttcgggg cgataaccat ggttaactga cgcccttcca ttttggcgtg
     7441 ctgctccaca gtacccatat cctccaagtc tttttcgatg cgctgcatca tttccat
//
LOCUS       DAOQGS010000156         7495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4758_length_7495_cov_4.260349, whole genome shotgun sequence.
ACCESSION   DAOQGS010000156 DAOQGS010000000
VERSION     DAOQGS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4758_length_7495_cov_4.260349"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="PKZ52_07630"
     CDS             complement(<1..1899)
                     /locus_tag="PKZ52_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG59682.1"
                     /translation="MRGLVSRQLLGVVLSLDIALQPLWALAADGAVASTAANAATQVT
                     QAHNGVEVIQIADPNAAGVSHNLFQDYQVGPQGQVLNNSATDVRSKLAGTIEGNSQLQ
                     HGSARIILNEVTGANPSSLLGVTEVAGQAASLVIANPNGITCSGCGFLNTPRVTLGTG
                     RSLWQDGQLTGLTVDQGTLEIGQEGLLGGNLERLELLAKQIVLYGQVVSSDLTLGAGS
                     LQFNFADGSIQKRNAQAGTTSLPQFAIDASALGGMYANKIALVATDNGVGVNVEGPMA
                     AYNGSIRIDANGEVRVNDVQSQQALKVETQGKVTLDKALATEIDVRTPDAVQVNGTLA
                     AQEQLSVQAKLIKSQGTVSAGYRADGTSNSAASLSLITTDKADNQGQWLSAGSLSINA
                     GDLTNRLGAMISGREGLSLTASAGSNRGELNSLGALTLTIDGLWDNRSGRVQTAGQLT
                     LNAETWDNRTGHLQGAVITADLDLNLNNAQGEVVTTSGDLQLNAADLNNQQGVLQSQA
                     AVRLTADTLDNTQGTLAAKGGEISRLALTQTLTNGGGNLTSASGTTEISAKTLDQTAK
                     ATGLAGGVIEFTGSGNHQITADQINNQGGQINNRGASLAIKAQDLHNQNGTLRSGQKT
                     DTTSATSAT"
     gene            complement(1965..3629)
                     /locus_tag="PKZ52_07635"
     CDS             complement(1965..3629)
                     /locus_tag="PKZ52_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNG59683.1"
                     /translation="MPRISLLVVLISFGLSSPLRAAEASGGTGASGEALIQQEQQRQE
                     TLRREREATDAPFTVIPRGRLTPEAPQADTGPCFDIQTIAVEGVEAFSSGRIAEVYSP
                     FLNRCLAAGDINSLIRSLTNLYLDAGYITSRAYLPNQNLTQGQLRVVVVEGFVESVTI
                     EGADAKRLRSAFPGIEGKRLNLRSIEQGIDAIGRSGVEAKVDFLPGTKLGATRLVVKV
                     KNRPERQFTLGIDNEGSKATGTTRVSLTQAWNNPVGYNDLVWVNYQTSALGRDDRRKA
                     TSLAASYSIPWGNWVETLSLNRYQYESWVSEAVVPFATSGDSSSISLDSRYLLWRNQT
                     GLTHLGLRLKQQDTENRVSDVRLITSSRTYSAASAYASYRHVEQRQEWQTELALHQGL
                     SSDQADLGAATPDTHFNKLTLDASYLKRSQWQLLPLEWRSQWQVQQSDSRLVASEQLS
                     LGGQYTVRGFAEQSVQGASGFFTRNSLSTLLHGRLDSAGFWQPSIALDYGQLRSGESL
                     SGAALGLSYTGNQLQLQLEVGKALRTEALQAEGFITQGSMSLSWRW"
     gene            complement(3837..4769)
                     /locus_tag="PKZ52_07640"
     CDS             complement(3837..4769)
                     /locus_tag="PKZ52_07640"
                     /inference="COORDINATES: protein motif:HMM:NF017489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59684.1"
                     /translation="MKPLNHLMLVILIGCFVTACVKVDVCRWAITPSVDSVLYNHGYI
                     YKAPNFPKENQPPGSSIAQNRLVSEDNVVDLSVTHLIIPGQKTALVYCGGNGFRQGTL
                     AKPLFNTFEPPPNMLIFDYPGYGQSGGEATAQEFDQATHLLGKHLSEWQQVHGFQRII
                     FWGRSFGGTICARLAGGYSGESSLVLETTYNDLRSLTSSRAGLFKHFFKIKIDSEAPD
                     FSIDKSLMSYSYPIAILKSTNDPITPPEASENLARLLVRGGKSVITVNMGAVDHKEIL
                     FQPCLANSILERIRPMVGDIRVRTENCTRKSGYE"
     gene            complement(5012..5602)
                     /locus_tag="PKZ52_07645"
     CDS             complement(5012..5602)
                     /locus_tag="PKZ52_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59685.1"
                     /translation="MNTHTLIAELDDVKPEHVLRDPRVVSRAIFSDSVARHGKYYLIL
                     HEQQHYIFVEAFQHVNKTINSSGEEWSGGTQIEIPEQALSWVLSQIDDHQAQIMSAGR
                     ENSGRSVVLPIYSGVSGSVLGEQLNVVRMKNDSGYVLENRSRRVHLPWANANWHQKLE
                     LTDQVLFEQGYWLVLKKLAARQASIAEDAELSNSEA"
     gene            complement(5700..6185)
                     /locus_tag="PKZ52_07650"
     CDS             complement(5700..6185)
                     /locus_tag="PKZ52_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59686.1"
                     /translation="MRKAIQDDQWSLNPIPFLTPITCCEHGFVYFGTWPTHIPEPYCV
                     YKDARYLVIQLDVFAAQATIGRPEVTRQLVIPRTALSWLAYRVRHPFTQREGYEGVFD
                     GEVLKVHRNATHAGEGVTGFVIQNLSRTSVKRQPQQLLISDDFMHEYLVPLMDALNCG
                     S"
     gene            complement(6178..6687)
                     /locus_tag="PKZ52_07655"
     CDS             complement(6178..6687)
                     /locus_tag="PKZ52_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59687.1"
                     /translation="MQDFFSIQPERATSDPRVVLHFLADDSAVPLGKHYVTLLDGAHF
                     IFVTAYQCAGQMPPMEDAWEGGSQLELPVLALKPLTTQMAWHFYTVSHRTLRSQAVFR
                     HEELFAGETLSLGRDYVTPGYTIRNHARKVHLPWASEEWVQEFRLADNLLFEQGYHAA
                     LTQLANDYA"
     gene            6990..7292
                     /locus_tag="PKZ52_07660"
     CDS             6990..7292
                     /locus_tag="PKZ52_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG59688.1"
                     /translation="MSVFAKRLKEIRKLRGLSQEKLGTLAGIDEASASGRMNHYERGR
                     HEPDFTMVERLAAVLKVPEAYFYAKEDELAQLLRTWPLLKKAQRQTINEIVKDLLT"
BASE COUNT         1836 a         2025 c         1817 g         1817 t
ORIGIN      
        1 agttgcgctg gtagcggacg tggtatcggt tttttgcccg ctgcgcaggg tgccattttg
       61 gttgtgaaga tcctgcgctt tgatcgctaa agaggcgcct cggttattga tctgcccgcc
      121 ctgattgttg atctgatccg ctgtaatctg gtggttgccg cttccggtga actcgatcac
      181 cccaccggca agccctgtgg ccttggcggt ttggtccagg gttttggcac tgatttcggt
      241 ggtgccgctg gcggaagtga ggttgccacc gccgttggtg agtgtttgag taagcgcgag
      301 acgactaatt tcaccgcctt tggcagccaa ggtgccctgg gtgttatcga gggtatcggc
      361 ggtaagcctg acagccgctt ggctttgcag cacgccctgt tggttattca ggtcagcagc
      421 gttgagctgc aaatctccgg aggtagtaac gacttcgccc tgagcattat taaggtttaa
      481 gtccaggtct gcggtgatga cggccccttg caggtggccc gtccggttgt cccaggtctc
      541 ggcattgagt gtgagctgcc cggcggtttg cacacgccca gagcggttat cccacaggcc
      601 atcgatggtg agggttagtg cgcccaagct attgagttcg ccccgattac taccggcgct
      661 tgcggtaagg cttaacccct cacggccgct aatcatggca cctaaacggt tagtgagatc
      721 acctgcattg atcgacagcg aacctgcact cagccattgt ccttggttat ccgccttatc
      781 tgtcgtgatc aggctcaaac tggccgcgct gttggatgtg ccatccgccc ggtaacccgc
      841 tgaaacggta ccttggcttt ttatcaactt ggcctgaaca ctcagttgtt cttgggcggc
      901 cagggtgcca ttgacttgaa ctgcatccgg tgttcgcaca tcgatctcag tggcgagcgc
      961 cttatccagc gttaccttac cctgagtctc aaccttgagc gcctgctggg attgcacatc
     1021 gttaacgcgc acctccccat tggcatcgat gcgaatggag ccgttatagg ccgccatagg
     1081 cccttctaca tttaccccca cgccgttgtc ggtggccacc agggcaattt tattggcgta
     1141 catgccaccg agggccgagg cgtcaatggc gaattgtggc aaagaggtcg tgcctgcttg
     1201 cgcattgcgc ttctgaatac tgccgtcagc gaagttgaac tgcaggcttc cggcgccgag
     1261 ggtgaggtcg gagctgacca cttggccgta gagcacgatc tgtttcgcaa gcaactccag
     1321 ccgttctaaa ttgccgccga gcaggccctc ttggcctatc tccaaggtgc cctgatcaac
     1381 agtgagcccc gttaactgac cgtcttgcca gagcgaacgg ccggtgccta aggtcacgcg
     1441 cggagtattt aaaaagccac agccggaaca ggtgatgccg ttggggttag cgatcactag
     1501 gctagcggct tggccagcga cttccgtaac ccctaacagg gaggacggat tagcaccggt
     1561 gacttcattg aggatgatgc gggcggagcc gtgttgtaac tggctattgc cttcgatggt
     1621 gcccgcaagc ttagagcgca cgtcggtggc gctgttgttg agcacttggc cttgggggcc
     1681 gacttggtaa tcttgaaata ggttgtggct gacgcccgct gcattggggt cagcgatctg
     1741 gatcacctcc acgccattgt gggcttgggt cacttgtgta gcggcattgg ctgcggtgct
     1801 ggctaccgcg ccatcagccg ccaaggccca caggggctgc aaggctatat ccaggcttaa
     1861 aaccacaccc aacaattgtc ggctaactaa tcccctcatg ggacggatcc tcgtgcttaa
     1921 tccagtttta ttgcgtttga tgtacataat tcagttgacc gggactacca gcgccaggaa
     1981 agactcatgc tgccctgggt gataaaaccc tctgcctgca aggcttctgt acgcagggct
     2041 ttgccgactt ctaactgcaa ttgcagctga ttgcccgtgt aactcagtcc cagcgctgcg
     2101 ccgctcaatg actccccgct gcgcagctgg ccataatcca gcgctatgga cggctgccaa
     2161 aacccggcac tgtccaaccg cccatgcaac agggtactaa ggctattgcg ggtaaaaaaa
     2221 cctgaggcgc cctgcacact ctgctcagca aacccacgca cggtgtattg gccgccaagg
     2281 ctgagctgtt cacttgccac cagccgactg tcactctgct gcacttgcca ttggctgcgc
     2341 cattctaacg gcagcagctg ccattggctg cgttttaggt aggaggcatc taaggtcagt
     2401 ttattgaagt gagtatctgg agttgccgcc cccaggtcag cctgatcgct gctcaagccc
     2461 tgatgaagtg ccagttctgt ttgccattcc tggcgctgct caacatgacg gtaactggca
     2521 taggcgctgg ccgcgctgta ggtgcgggag ctggtaatca agcgcacatc gctgactcgg
     2581 ttttccgtgt cctgctgttt aagccgcagg cccaggtggg ttaaaccggt ttgattgcgc
     2641 cagagtaaat agcgactgtc caaactgata ctggaagagt caccggaggt ggcaaagggc
     2701 accaccgcct cactcaccca gctttcatac tggtatcggt taaggctgag ggtttctacc
     2761 caattgcccc aaggaatgct gtaactggcc gcaaggctag tagccttgcg gcgatcatca
     2821 cgccctagag cggaagtttg atagttaacc cagacaagat cgttataccc tactgggttg
     2881 ttccaggctt gcgtgagtga aactcgagta gtgccggtgg ccttggagcc ctcattatcg
     2941 atacccagag taaactgccg ctcagggcga tttttcactt ttaccactaa ccgcgtagca
     3001 ccgagcttag tacccggcag aaaatccacc ttcgcctcca cgccgctgcg gccgatagca
     3061 tctatgccct gctctattga acgcaagttc agccgctttc cctcaatacc ggggaatgct
     3121 gaacgcagcc gttttgcatc ggcgccttcg atggtcactg actccacaaa cccttccacc
     3181 accacgacac gcagttgacc ttgagtcagg ttctgattgg gcaaataggc gcggctggtg
     3241 atgtagccag cgtcgaggta aaggttggtg aggctgcgaa ttaacgaatt tatgtcaccg
     3301 gccgccaaac aacggtttaa aaatggacta taaacctcgg cgatacgacc tgaggaaaac
     3361 gcctctacac cttccaccgc aatggtctga atatcaaaac acgggccggt atccgcctgg
     3421 ggagcttccg gtgtcagccg tccacgggga atgacggtaa atggggcatc cgttgcctcg
     3481 cgttcgcgcc gcaatgtctc ttggcgctgt tgttcttgct gaatcagagc ttcgcccgac
     3541 gctccggtcc ctccagatgc ctcagcggcc ctcaaaggac tggataaacc aaaactgatt
     3601 aaaacgacca gtaaactaat ccttggcatt aaaaagactc tgaattcggg ggcgccattt
     3661 taataccgga gcagcaaata aggccatcat ttgaaagctt tttatccaag taattttctg
     3721 taaaacaccc cattagggtc tgccccttac acgatacacg ttctgttttc gctcaaaaag
     3781 tctgcacgac taaatcttca tgtaggtgca atttctatgt cgggtgaaat tttgcgctat
     3841 tcataacctg attttctcgt acagttttca gtccgcacgc gtatatcccc taccataggc
     3901 ctgatacgct ctaaaataga gttcgccaag cacggctgaa atagaatttc tttgtgatca
     3961 acggcaccca tattcacggt aataaccgac ttaccaccac gcactaatag tctcgccaaa
     4021 ttctccgacg cctctggcgg ggtaatcggg tcattcgtag atttcaaaat agctatgggg
     4081 tacgaataac tcattaacga tttgtctatc gagaaatcag gggcttcaga atctattttt
     4141 atcttgaaaa aatgcttaaa caaacccgcc ctggacgatg tcagcgaacg caaatcatta
     4201 taagtagttt ccaataccaa agaactctca ccagaataac ctcccgccaa acgggcgcag
     4261 attgttcctc caaaagagcg accccaaaat ataatgcgtt gaaatccgtg tacctgctgc
     4321 cattctgaaa ggtgtttgcc tagcaggtgt gtagcctgat caaattcttg cgctgttgct
     4381 tcaccaccgg attgaccata tcctggataa tcaaaaatca acatattggg tggtggctcg
     4441 aaagtattaa ataggggctt tgccaaagtc ccctgacgaa atccattgcc accacaatag
     4501 accaacgcag tcttctgacc aggaataatt aaatgagtga cggataagtc gacgacatta
     4561 tcttcactaa caagtcgatt ctgtgcaatg gaggatccag gtggttggtt ttcttttgga
     4621 aaattcggag ccttataaat atacccgtga ttgtataaaa cggaatcaac agacggcgta
     4681 atggcccaac ggcacacatc tacttttaca caggcggtga cgaaacagcc aattaaaatt
     4741 accagcatca gatggtttag cggtttcaat ttgattctca tccatcgctt acttagctaa
     4801 attcattcga gtaaaaaaca tttgacacat cttgaatcag accttttatt ggtctggcgc
     4861 gccaaactca caacccttga acaccggctc catgattaat aattcaaatc tgagccaaac
     4921 cccaaattag tacctaacat gggacttgat attgctgcct cacccctaaa accgtctgat
     4981 tactgcaggg acacaatcat taatacaaaa atcaagcttc actgttagaa agctccgcat
     5041 cctcagcaat cgaggcctgt cgggctgcca gtttttttaa aaccaaccaa tacccttgct
     5101 caaacaatac ctgatccgtc agctctaatt tttgatgcca attggcgttg gcccaaggta
     5161 aatgcacacg acgagatcga ttctcaagca cataacccga atcattcttc atgcgcacca
     5221 cattcagctg ttcacctaaa acacttccgc tcacgccgct ataaataggc aacacaacag
     5281 atcgccccga attttcccgt ccagcactca ttatctgcgc ttggtgatcg tcaatctggc
     5341 tcaataccca cgaaagcgct tgctcgggaa tttcgatctg cgtgccgcca ctccactcct
     5401 caccactgga attaatcgtt ttatttacat gctgaaacgc ctcaacaaaa atataatgct
     5461 gttgctcatg caaaattaaa taatattttc catgtcgtgc cacagaatca ctgaaaatag
     5521 cgcgcgatac gacgcgcgga tctcgcaaga cgtgttcggg ctttacatca tccagctcgg
     5581 caattagcgt gtgcgtattc ataggcgtcc tctcgcatcc attggggcgt gttaatgttt
     5641 aatatctatc tgctcgacga cggcatacga aatgcgggcc atccaatatt tatacaccct
     5701 catgaaccgc aatttaatgc atccatcaag ggcactaaat attcgtgcat aaaatcatcg
     5761 ctaatcagaa gctgctgggg ttgacgctta accgaggtcc ggctcagatt ctgaattaca
     5821 aatcccgtca cgccctcacc cgcatgagtg gcgttacgat gcacctttag aacttcgcca
     5881 tcgaaaacgc cctcatagcc ctcgcgctgc gtgaaggggt gacgcacccg atacgcaagc
     5941 caactcaacg cggtacgagg gatcaccaac tggcgtgtga cctccggtcg ccctatcgta
     6001 gcttgagctg cgaacacgtc cagttgaatt accaagtaac gcgcatcttt gtatacacaa
     6061 tagggttccg ggatgtgcgt cggccaggtt ccaaaataca caaaaccatg ttcacaacag
     6121 gttatcggtg tcaaaaaggg tatggggttg agggaccatt gatcgtcctg gattgcttta
     6181 cgcatagtca ttcgccaatt gtgtcagtgc ggcatgataa ccctgctcaa ataacaggtt
     6241 atcggccaat cgaaattcct gaacccattc ttcactcgcc cagggtaagt gcaccttacg
     6301 cgcgtgattt cgaatcgtat agcctggtgt gacatagtcc cgaccgagac taagagtttc
     6361 acccgcgaaa agttcctcat gccgaaaaac cgcctgactg cgcagtgtac gatggctgac
     6421 ggtataaaaa tgccacgcca tttgggttgt gagtggtttg agcgccaaca ccggtagttc
     6481 aagctgactc cctccctccc aggcatcctc catagggggc atctgccccg cgcattgata
     6541 ggctgtgaca aaaataaagt gcgcaccgtc taacaaggtc acgtaatgct tacccaaagg
     6601 cacagccgag tcatcggcta gaaaatgcaa aacaactctg ggatcactcg tagcccgctc
     6661 gggctgaatc gagaaaaaat cctgcacgtt gacctttcac tcattgccaa ttggggccta
     6721 ggttccattg gcggtttatt catctagaaa tccgcgcgca ctgtaacggc caaatcagtg
     6781 tctaacaagt aggcggtaac gctgattttt gtggagttac cggattgatt ggcgcggtca
     6841 attcaaaacg tagctctggc caacatcaac caccgctctg tctcatgcac ggctcaacag
     6901 gctatcagat tttacaaaac ttaggaatcc tatgcaaaaa ccctaacaca tccaatgaag
     6961 atcactgcct tcttaaatga gggcaatcca tgtccgtttt tgcgaaacga ttaaaagaaa
     7021 tacgcaagct gagggggtta tctcaagaaa aactgggcac cctggctggc atcgacgaag
     7081 cctccgctag tgggcgaatg aaccattatg aacgcggtcg acacgaacct gatttcacaa
     7141 tggtcgaacg gctggcggct gtattgaaag tgcccgaagc ctatttttat gcaaaggaag
     7201 atgaactcgc tcaactgctt cgaacctggc cactccttaa aaaagcacag cgacaaacca
     7261 ttaacgagat agttaaagat ctcttaacct agaactcgct gcatgtccca ataacactcg
     7321 ctgagtggat tcccctacca atgacaccat caaaccgctc gcaatgtccg gctgtaagtc
     7381 tttgatttta aagagaccaa atttttggac taccggactt cctactttgg tgcacgggag
     7441 aattcggaga atatttccaa ggagagagag aaaagaccta gaatgcttaa aaaat
//
LOCUS       DAOQGS010000157         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4778_length_7476_cov_4.754211, whole genome shotgun sequence.
ACCESSION   DAOQGS010000157 DAOQGS010000000
VERSION     DAOQGS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4778_length_7476_cov_4.754211"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PKZ52_07665"
     CDS             <1..1160
                     /locus_tag="PKZ52_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59689.1"
                     /translation="ADGKTDEPLWLRDLTANQPPADGVFHGLIPGLSKQGVVSLSLIA
                     DGKTFKREYRQRISVRERFSAELEPVSDNGYELVVKTYDESIDKNSVTFLAQVETPAH
                     EKQPLTLNITPVDTWVGAIELPAPGAYTVKITAKATGKNGAAVLHEFAPIPIAHQLDK
                     VIKEAPPQPEVHATPVAETPTPPPAAEPAPTGGMPSWAFYALLAGANLGLVGLAYFLY
                     KKFVGGKSEEEVLSAFSLEPEPEAPVEKPKKAPPPPPPMEEYDETQMQIPTTEEEIPP
                     MEDLDPDLDMSTDVDDTAASSSEVDIDALLAEQADEPGPKKDEADDMVQAMLRAQGLD
                     LAEDEMDDAISSLIDELDGAESKADLTADTNGGDDEFDNFDDFGMDDEDKT"
     gene            complement(1283..2269)
                     /locus_tag="PKZ52_07670"
     CDS             complement(1283..2269)
                     /locus_tag="PKZ52_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNG59690.1"
                     /translation="MKILFIGGTGNISAAVSQQLVALGHELWLLNRTGAAGIQGAHSL
                     IGDIHSPASLAQLHRHHWDVVVNWIAFNPADIQRDIALFRDKTQQYVFISSASCYQNP
                     GPTALITERTPLANPYWEYSRNKIAAEQLLMQSVKEFGFPATIVRPSHTYSRVIPLTI
                     GGWTEYTCVARMKKGLPIVVQGDGTSLWTLTHARDFAVGFIGLLGRSEAIGEDFHITS
                     DEFLSWNEIYQLTAAAVGVQADIVHVTSERICQFDADYTGTLLGDKSVSALFDNSKIK
                     RLVPHFAAQTPFKDGIKETLAWFEAEPSRQIIDPETDALIERLVADARRPLV"
     gene            complement(2467..3360)
                     /locus_tag="PKZ52_07675"
     CDS             complement(2467..3360)
                     /locus_tag="PKZ52_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59691.1"
                     /translation="MRFCFILLLLLNAHTALSAQALRLDTNSNIEVVFPDKNPVIFVG
                     KKSLDKSANNGGGIMYPGDTAGLFLVSVLTHAAISGAAQSSAEKKEQELANQVLAPFA
                     DIIKEVDMNFLWDEQRIRAADPKLHDLVFNTQKSAIANSWSLDTKPVFALTQSKASLI
                     LYNKINLECAGDGSKKKSKNRSKKKPAESDGLTVVIISDPIIEENKTDYWLRNSGSAF
                     KNEIKRLFTLSIGMAIQSARDEVSISSLPEVSVRYLQDGLKYLERGQIITQDCRRTVF
                     KTLSNELKSVPNLDFTNCPQT"
     gene            complement(3411..4088)
                     /locus_tag="PKZ52_07680"
     CDS             complement(3411..4088)
                     /locus_tag="PKZ52_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59692.1"
                     /translation="MKYFAAFIILSIFSAGCATVPHQPTISFDSNTFKKGEKIGVYIS
                     PTPKITTQFPGANCLLCLGVAAAAHSGLTKQVESFKAESLGKVPDVVNNYLKGKGIEV
                     VKINEMIKKLPKVKPSSGSTLAQNYGPYKTQYGIDRLLVINYQRVGVDRPYSAYVPTA
                     VPHAVITAEFYIVDLATNSYSAYSPISITKEPDGSWDVAPKYPAITNAFYQAEEQASD
                     LLTKPFK"
     gene            complement(4573..5796)
                     /locus_tag="PKZ52_07685"
     CDS             complement(4573..5796)
                     /locus_tag="PKZ52_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG59693.1"
                     /translation="MSVSPLFARQPIFTAKLEVVGYELLFRSSQQNRADFIDGDSASS
                     HVLLYAFGEHRINDVIGDKKAFINFTRNLLLGNAPLPPEQLVIEILENITCDKLLLEA
                     LKEKKQQGYEFALDDFVLTKDNNALLPFASTIKVDVLNTSEAEITRIIRAAKPYAIRL
                     LAEKIETHAMFKHCVELGFEWFQGYFLSKPQIVEGVKTSGNIQALLQLLASLMAPEVN
                     IDTVCTAITSDARLTYKILKLANGASKNQAAKFGSIQQAVLLLGLNAIRNWATLLLLT
                     SNDSEPQEIYTQSCARAKLCELIGQQIGDKTFAENCFTLGLLSTLDVYLKMPIKAIAQ
                     QLSLSEEMHTGLIKHTGKLGHVLAISLAIEQQQWAKVTPAASHYKIPERILSDCYIQA
                     YQWANQIQADIKERH"
     gene            complement(5805..6368)
                     /locus_tag="PKZ52_07690"
     CDS             complement(5805..6368)
                     /locus_tag="PKZ52_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59694.1"
                     /translation="MESQNECSTIESWSHVRETINMLYLAVCQIETTMADSNTSVDTL
                     TRSFTLLANHTSEVSEQIQNLTEPAQLEAFKKDIDSTAQEMKTNISASICAFQFYDRV
                     CQRLDHVSRSLEKVSAVMASRESLCQPDAWRKIQMDIKSSYTMEAERLMFDYIMQGGT
                     VAEALKIYREQVDASGNKNNEDEIELF"
     gene            complement(6421..>7476)
                     /locus_tag="PKZ52_07695"
     CDS             complement(6421..>7476)
                     /locus_tag="PKZ52_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNG59695.1"
                     /translation="AQQFISQSYALRETSLVEELIRVSLENMDWLKVNEGILMLPTTA
                     QKTERWQYWRARALDELKLEQADFGPTINVYSTLAEKRGFYGFLAADILRKHYSLEDK
                     SKPMDQVSFMRVNQLDGIRRAKELWAMGYEDESRAEWNHTTKKLSPDELYAAGALAEQ
                     WGWYATSIRTMTKGNLWDNLTSRFPLAYANEVKRAAQNKNLHPTLVFAIARQESSFAA
                     DAKSPVGALGLMQLMPATAQEVAKKQGLSHKTADLLNPGYNLLLGTTFLNELLERFDG
                     NRILAAAAYNAGPGRVRQWLGESQQQRPFDVWIETIPFNETRQYVQNVLSFSVIYGYR
                     LGQPQSLVSQAEAKQRL"
BASE COUNT         1812 a         1697 c         1928 g         2039 t
ORIGIN      
        1 ccgccgatgg caaaaccgat gaacccctgt ggctgcggga tttaaccgct aaccagccgc
       61 cagccgatgg cgtgttccac gggctcattc caggccttag caaacagggc gtggtgagcc
      121 tcagcctgat tgccgacggt aaaacattta aacgggaata tcgccagcgc atcagtgtgc
      181 gcgagcgctt tagcgctgag ttagaaccgg tttctgataa tggttacgaa ctggtggtaa
      241 aaacctacga cgaatccatt gataaaaatt cggtgacttt tttagcgcaa gttgaaaccc
      301 cagctcacga aaaacagccg ctcacgctta atattacgcc ggttgatacc tgggtggggg
      361 ctattgaatt gcccgcgcca ggcgcctaca cggtaaaaat tacggccaaa gctaccggta
      421 aaaacggcgc cgccgtttta catgaatttg cacccattcc aattgcacac caactggata
      481 aagtcattaa agaggcaccc ccccagccag aggtacacgc aacaccggtt gccgaaacac
      541 ctacaccgcc ccccgctgcc gaacctgcgc ctaccggcgg tatgcccagt tgggcgtttt
      601 acgccttgtt ggctggagcc aatttagggc tagtcggctt agcctatttt ttgtataaaa
      661 aatttgtcgg cggtaaatca gaggaggaag tcttaagcgc ttttagccta gagcctgaac
      721 ctgaagcgcc ggtggaaaaa ccgaaaaaag caccgccacc tccacccccc atggaagaat
      781 atgacgaaac ccaaatgcag atacccacca cggaagaaga aattccccca atggaggatt
      841 tggaccctga cttagatatg tccacggatg ttgatgacac tgcagcgtcc agctcagagg
      901 ttgatataga cgctttatta gcagagcaag cggatgaacc tgggccgaaa aaagacgagg
      961 ccgatgatat ggtgcaggct atgttgcgtg cccagggcct ggatttggcc gaagacgaaa
     1021 tggatgatgc aatttccagt ttaattgatg aattagatgg tgctgaaagc aaagccgact
     1081 taacggctga taccaatggc ggcgatgatg aatttgataa ctttgacgat tttggtatgg
     1141 atgacgagga taaaacctaa tttagaggcg tctggttatt aatccggcga gttaatcaat
     1201 ctggatatcg ggatattttg caaccctagc gggcaggacg cccgcaacag ggcgacgcat
     1261 acagacctaa tcagaccatt ctttatacaa gcggcctgcg cgcatcagcc actaaacgct
     1321 caattaaagc atcagtttcc gggtcaataa tttgtctgct gggctcggct tcaaaccagg
     1381 ccagtgtttc ttttataccg tctttaaaag gtgtttgggc ggcaaagtgc ggcaccaggc
     1441 gttttatttt gctgttatca aacagcgcac tcactgattt atcccctaat aaagtaccgg
     1501 tgtaatccgc gtcgaattga caaatgcgct ctgaagtaac atgcacaatg tccgcttgta
     1561 cgcccacagc ggcggcggtt aattgataaa tttcattcca gcttaaaaat tcatctgacg
     1621 tgatatgaaa atcttcgccg atcgcttcgc tgcggcctaa taatccgata aagccaactg
     1681 caaaatcccg cgcgtgggtc agtgtccaga gtgatgtgcc atccccctgt accacaatcg
     1741 gtaaaccttt tttcatacgc gcgacgcaag tatattcagt ccagccgcca atggtgagcg
     1801 gaatgacccg gctgtaggta tggctggggc gcacaatggt ggcgggaaat ccgaattctt
     1861 taacgctctg catgagcagc tgttcggcgg caattttatt gcgcgaatat tcccaatagg
     1921 gattggccag cggagtgcgc tcggtaatta aagccgtggg gcctgggttt tgataacagg
     1981 aggctgaact gataaaaaca tactgctggg ttttatcgcg aaatagggct atatcgcgct
     2041 gaatatctgc agggttaaac gcaatccaat tcaccactac atcccagtgg tgcctatgca
     2101 attgtgcaag gcttgcaggg ctgtgaatat cgccgataag actgtgcgcg ccttgaattc
     2161 ccgccgcacc agtgcgattt aataaccaca attcatgccc gagggcgaca agttgttggc
     2221 tgacggcggc gctgatattg ccagtgccgc caataaataa gattttcata ggcacaattt
     2281 acgcaagaat aatagaggcg gcattttata atgtctgtcg atgagccgct aggggcgtgt
     2341 gttgatgcgt aagaattcgt ggcgaatctc tggcgaatta atgtttgcga aaaaagcacg
     2401 agcacgtatt tgtagggggg gcagcaatat ccttgctgcg cgagctttcc gaaagctgtt
     2461 aagctgctaa gtttgagggc agtttgtaaa gtcgagattg ggaacagact ttaattcgtt
     2521 actcaaagtt ttgaacactg ttcttctgca atcttgcgta atgatttgac ctctttcgag
     2581 atatttgagt ccatcctgga ggtagcggac ggagacctca ggtaaggatg atatcgaaac
     2641 ttcgtccctt gcggactgaa tcgccattcc aatcgataaa gtgaataatc ttttaatctc
     2701 gtttttaaaa gcactgccgg agtttcttag ccagtagtct gttttgtttt cttcgataat
     2761 ggggtctgag atgattacca cagtaagccc atcgctttca gctggctttt ttttgctgcg
     2821 atttttgctt tttttcttcg agccatcgcc tgcgcattct agatttattt tattgtataa
     2881 aattagactg gccttgctct gtgtgagtgc aaaaactggt ttggtgtcga gagaccagga
     2941 gtttgcaatt gcgcttttct gtgtgttaaa tactaggtcg tgcaatttgg gatctgctgc
     3001 acggatcctt tgctcgtccc aaagaaaatt catgtctact tctttaatga tatcagcaaa
     3061 aggagcaagt acttgatttg ctaattcttg ctcctttttt tcggctgacg attgcgcggc
     3121 gccacttatg gctgcgtgcg ttaaaaccga aactaagaac aaaccggctg tatcacccgg
     3181 atacattatg cccccgccat tgttggctga cttatctagg gatttcttac cgacaaaaat
     3241 aacggggttt ttatctggaa acaccacctc aatgttggag tttgtgtcca ggcgtagagc
     3301 ctgcgcagat agagcggtgt gggcatttag caataacagt aatataaagc aaaagcgcat
     3361 gagatggacc tgaaaagggt ggcctattag cccccctgga aacaattatt ttatttgaat
     3421 ggcttcgtta acaaatcaga tgcttgctct tcagcttgat aaaaagcatt tgttattgca
     3481 gggtatttag gagcgacatc ccaggagccg tcaggttctt tggttatact tattggcgaa
     3541 taagcggagt aagagttcgt cgcaaggtca actatataga attcggcggt aatgaccgcg
     3601 tgcggcactg cagtaggtac atatgcgcta tacggacgat caactccaac acgttggtaa
     3661 tttattacga gtagacgatc aattccatac tgagttttgt atgggccata gttttgagcc
     3721 agcgttgagc cggaagaagg ttttaccttg ggtaattttt ttatcatttc attgattttg
     3781 acgacttcaa ttcctttgcc cttaaggtaa ttgttcacca catcgggaac cttgcctaaa
     3841 gattcagcct tgaatgactc tacctgttta gtaaggccac tgtgcgctgc ggcagcgact
     3901 cctaagcaca gtaggcagtt tgcgccaggg aactgggtgg taatttttgg cgttggacta
     3961 atgtatacac caattttctc gcctttttta aaggtgttgc tatcaaagct aatggtaggt
     4021 tgatgaggga ctgtagcgca gcctgctgaa aaaatgctta aaatgataaa cgcagcgaag
     4081 tacttcacaa atatctcctg aaatgtttta tataggcttg attacagttg ctaccaagaa
     4141 gttaacaatg ggtagtgtga ttgtttgttg ctaaagtggc aatgttaaat ttgcactcat
     4201 caaccttagt aaaatcacat aaaatggtca agtaggacta gtgattcgcc tctgatttat
     4261 tgtgggtaga cagtatagat caccttgaag attgtctgat tgatctcggc cacattaatt
     4321 cgttttgaat ggggcgaaag gtgaaggtga gctaggttcg cctccgacgc tcattcccgc
     4381 cccatgcgtt gttgtttact tggtaggtca gtctgcattc agataaattg ccagtgggtt
     4441 tggaaccttt tatcgcgtca tcccgcgcaa caagttgtcc ccacgaacat ttcttgcgat
     4501 catcatgaaa atcgtgtttt tccaattgtc aatctgtgta aacactcatc caatcagcgc
     4561 gtagagtgcc acttaatgac gctccttaat atccgcttgt atttggttgg cccattggta
     4621 ggcttgaata taacagtcgc ttaatatgcg ctcagggatt ttatagtggc ttgcggcagg
     4681 ggtaactttg gcccattgct gctgctcaat ggcgaggcta atagccaaca catggccgag
     4741 tttgcctgtg tgttttatca ggcctgtgtg catctcttcc gagaggctta attgctgagc
     4801 tatggctttt ataggcattt ttaagtagac atccaaagtg gataaaagcc cgagggtaaa
     4861 acaattttcc gcaaaggttt tatcgcctat ctgttggcca attaattcgc acagttttgc
     4921 gcgggcgcaa ctttgggtat agatttcttg cggctcgctg tcattgctgg tgagcaataa
     4981 cagcgttgcc cagttgcgaa tggcgttaag gccaaggagt aaaactgcct gttgaatgga
     5041 gccgaattta gccgcttggt ttttgctagc gccgttggca agttttaata ttttataagt
     5101 gaggcgtgca tcggaggtaa tggcggtgca gacggtatca atgtttactt ccggcgccat
     5161 taaggaggct aatagttgta gcagcgcttg aatattaccg gaggttttca cgccttcgac
     5221 gatctgcggc ttagataaaa aatagccttg aaaccattca aagccaagct ccacacagtg
     5281 tttaaacatg gcgtgggttt ctattttttc tgccaagagg cgaatggcgt agggtttggc
     5341 cgcgcgaata atgcgcgtta tttccgcctc cgaggtgttt aatacgtcca ctttaatggt
     5401 tgaggcaaag ggcagcaggg cattgttgtc tttagttaac acaaaatcgt ccagggcaaa
     5461 ttcataaccc tgttgttttt tttctttaag tgcttctagc agcagtttat cgcaggtgat
     5521 gttctccaaa atttcaatca ctaattgttc tggcggcagg ggtgcgttgc cgagcagtag
     5581 attgcgcgtg aagtttataa aggctttttt atcgcctatg acatcgttaa tgcggtgctc
     5641 gccgaaggca taaagcaata catgggaact cgcgctatct ccgtcgataa agtcggcgcg
     5701 attctgttgc gacgagcgaa ataacagctc gtaccccacc acctcaagtt tggcggtaaa
     5761 aatgggttgg cgggcaaata aaggcgagac gctcatggcg caaatcaaaa caattcaatt
     5821 tcatcttcat tgtttttatt gccgctggca tccacttgtt cccggtaaat ttttagcgct
     5881 tcagcaacgg tgccgccttg cataatgtaa tcaaacatta aacgctcggc ttccatggtg
     5941 taactgcttt taatgtccat ttgaattttg cgccaggcgt cgggctggca gaggctctcg
     6001 cgggaggcca taacggcgga gactttctct aaactgcggg agacgtgatc cagccgttgg
     6061 cagacgcgat cataaaattg aaacgcacaa atagacgcgc tgatgttggt tttcatttct
     6121 tgggcggtgc tgtctatgtc ctttttaaac gcctccaatt gtgccggctc ggtgaggttt
     6181 tggatttgtt cactgacctc gctggtgtga ttggccagca gggtaaacga gcgggtgagg
     6241 gtgtccaccg aggtattgct gtccgccatg gtggtttcta tttggcacac cgccaaatac
     6301 agcatattga tggtttcgcg cacatgggac caagactcta tggtgctgca ctcgttctgg
     6361 gattccatgt gtgctcctat tgcatgagtt gggcggttgc cgttcatgct taagcataga
     6421 tcacaaccgc tgttttgcct ccgcttggct caccaggctc tggggttggc cgagcctgta
     6481 gccgtagatt accgagaagc tcaaaacgtt ttgcacgtac tggcgggttt cattaaacgg
     6541 gatagtttcg atccagacat caaaggggcg ctgctgttgc gattccccca gccactggcg
     6601 cacccggcca gggcctgcgt tataggcggc ggcggccaga atacggttgc cgtcgaagcg
     6661 ctcaagtagc tcgtttaaaa acgtggtgcc gagcaaaagg ttgtagccgg ggtttaacag
     6721 gtcggcggtt ttatggctta agccctgttt tttggcgact tcctgggcgg tggcgggcat
     6781 taattgcatc agccctaaag cgccgacggg agatttagca tccgccgcga aggagctttc
     6841 ctgccgggca atggcgaaca ccagggtggg gtggaggttt ttgttctggg cggcgcgctt
     6901 cacctcattg gcataggcaa gggggaagcg gctggtgagg ttatcccaca ggttgccttt
     6961 ggtcatggtg cgaatactgg tggcgtacca gccccattgt tcggccaaag cccccgcggc
     7021 atagagttca tcggggctta attttttggt ggtgtggttc cactccgcgc gggattcatc
     7081 ttcatagccc atggcccaga gctctttggc cctgcggata ccgtctaact ggttcacccg
     7141 cataaaactc acctggtcca tgggcttgga tttatcctcc agggagtagt gtttacgcag
     7201 gatatccgcc gctaaaaagc cgtaaaagcc gcgtttttcc gccagggtgg aatagacgtt
     7261 aatggtgggg ccgaagtcgg cttgttcgag tttcagttcg tccagggcgc gggcgcgcca
     7321 gtattgccag cgctcggttt tttgcgccgt ggtgggcagc atcaaaatgc cttcattcac
     7381 cttcagccag tccatattct caaggcttac gcggatgagt tcctctacca ggctggtttc
     7441 ccgcagggcg taggactggc tgataaactg ctgagc
//
LOCUS       DAOQGS010000158         7437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4832_length_7437_cov_4.427120, whole genome shotgun sequence.
ACCESSION   DAOQGS010000158 DAOQGS010000000
VERSION     DAOQGS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4832_length_7437_cov_4.427120"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..2076)
                     /locus_tag="PKZ52_07700"
     CDS             complement(34..2076)
                     /locus_tag="PKZ52_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG59696.1"
                     /translation="MKFMLHRLAIALALPMAHQANATTPAKPQADNTVEEVLVLGSRK
                     GSYTEITENTEKLVNMPGSLGDPLGAITALPGVMTPAQGGAPAVRGSSPSDNRYYIDG
                     LPASYIFHDFNTSILDEHVIQDFQLFSAGFGAPYSNATGAIFDIRLRDPRHQDLSTTV
                     NLSFLRAGLFVEGAATEHSAFYLSLRQGMLQYLLSKDDKPDEDGVRIESPPSDDDYQF
                     KYVWDLSPQDQITLTLAGANDGIEADLSETNNFTARNPDFAGRAKIDKGFDTQGGRWQ
                     HNFASGGTAQLAVAQNNNTDTISWGEGYFFRTDFTNSLLRAAVSQPFGNHRLTLGSEL
                     NRYAYNYRIRMVNIVCTDFEPDCDGNRRGIIEGSRSVDVNYRMLYLTDNWQMTDAFNL
                     DLGLQQNHNDFTEENFLHPRAALTWQMVKQLALTASAGRYDRFPDLDTVMPLVGNPDL
                     KSPQADHFTLGLKGDFSERWNWSLEAYRKKLTELPLALDESQPDADLYYSNDIKGDAK
                     GVELMLNRERDEHWYGWMSLSWSQSERTNLRTGETRTYTLDTPLVFNLVGNYQFNDAW
                     SAGFRFSAKSGEAMTKIIGVRENPNFPGHYLPVYGEAYQDRLPIYSRLDLRMERKTQF
                     WGYPGSFYVDVINALNHRNVTQIDLDYEQVNKNGQLKLEESREMGIFPSVGVSMTF"
     gene            2811..3569
                     /locus_tag="PKZ52_07705"
     CDS             2811..3569
                     /locus_tag="PKZ52_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG59697.1"
                     /translation="MNNLSDWFIFLPFITLGLVSSAHCVGMCGGIMAALTVSITQPAQ
                     SKRWLLLVAYNIGRIGSYGLMGFVLALFSQLFHHWAGGIVVRLIAGALMIAMGLYLAN
                     IWRGLIHLERVGRYLWVYLQPLSKYLLPVDKGIKALALGALWGWLPCGLIYSALALSL
                     TQPSAALAGAAMLAFGMGTLPAVLMVGITAHHLSRLLQTRSVRLGLGGLMMLYGIWTM
                     SASIIYGGHQHGAQIEQPSSLNIEIQHRHHREPN"
     gene            complement(4236..4997)
                     /locus_tag="PKZ52_07710"
     CDS             complement(4236..4997)
                     /locus_tag="PKZ52_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG59698.1"
                     /translation="MQNTKIALITGGSRGLGKNMAIHLAKRGRDIIFTYHSNEQQAQA
                     TLAELSQLGVKALALQLDISQSSGFSAFADQLKAALKSHWGKNHFDYLINNAGSGIFK
                     PFEHTTPEEFDRMVNEHIKGPYFLSQALLPLINDAGRILNISSGLTRMTVPGYSAYAI
                     MKTAVEALTRYLAKELAPRRIRVNTLAPGAIETDFGGGAVRDNKELNQHIAEITALGR
                     AGLPDDIGGAAAALLDDETGWINAQRIEASGGQGL"
     gene            5194..6099
                     /locus_tag="PKZ52_07715"
     CDS             5194..6099
                     /locus_tag="PKZ52_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNG59699.1"
                     /translation="MRLDHFQTLGPILEQNSSQWGITPTQVPGVAIFKANSTTTPLPL
                     VYQPSLCILVQGEKHIVVGDETYRYQPGQLLAVTLDLPLHSQITAASEASPYLLLKIE
                     LDAALIAELYPFVHMEKFQEQPYAKGLFIGDLNEATMDAVVRLAELIANPQHIAVLAN
                     QTKREIFYRLLCGPQGALIAQIAVQDSYLQRIAKALQFIKEDFKAALSVPYLAQLAGM
                     SVSSFHAHFKNITGISPLQYQKSLRLMAARNLMLAEDMDAAATAYRVGYESPSQFSRE
                     YARMFGSPPGRDVQQLKQVKKLRAM"
     gene            complement(6345..6563)
                     /locus_tag="PKZ52_07720"
     CDS             complement(6345..6563)
                     /locus_tag="PKZ52_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNG59700.1"
                     /translation="MADDSKVKGPASYFPSIEKTYGKPISFWLDVLDSLQDKKHMEQV
                     AHLKSEYKIGHGHANALVAYHRGGVKAG"
     gene            complement(6753..>7437)
                     /locus_tag="PKZ52_07725"
     CDS             complement(6753..>7437)
                     /locus_tag="PKZ52_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNG59701.1"
                     /translation="VGEAQQADDHIKVLKNTFKSADGAGFTPFALAPYQAPIKQAWLT
                     NSQVHFCARAYATVPMDHEDSAPLVVLGNLLRNGFLHRAIREQGGAYGGGASQDSNSG
                     AFRFYSYRDPRLEETLADFDKAIDWFMHSQHSWQSIEESILGAVSGIDKPDSPAGRAK
                     RLFHADLHGRTLEKRQAFRDRLLATQLTDLQRVCERYLKPQLAHTAIVTDKTHAARAQ
                     ELGLELHWV"
BASE COUNT         1773 a         1780 c         1871 g         2013 t
ORIGIN      
        1 accctcgcta cgagttcaac tgcggttttt aggttaaaac gtcatagaaa cccccaccga
       61 cgggaaaata cccatctcgc gggattcctc caattttaat tgcccatttt tattcacctg
      121 ctcatagtcc aaatcaattt gcgtgacatt tctgtggttt aaggcgttaa tcacatccac
      181 ataaaaactg ccggggtaac cccagaattg tgttttgcgc tccatacgca ggtctaggcg
      241 cgaataaatg ggcagccggt cttggtaggc ctcgccatag actggcaaat aatggccggg
      301 gaaattaggg ttttcccgca cgccgataat tttagtcatc gcctcaccac ttttagcgct
      361 aaagcggaaa cctgcgctcc aggcatcgtt aaattgataa ttgcccacca ggttaaatac
      421 cagcggtgta tccaaggtat aggtgcgggt ttcgccagtg cgcagattag tgcgctcact
      481 ctgggaccag ctcagcgaca tccaaccgta ccaatgctca tcccgctcgc ggttgagcat
      541 taattccacg cccttagcat cacctttaat atcattgctg tagtacaggt cggcatcggg
      601 ttggctctca tctaatgcca ggggtaattc ggtgagtttt tttcggtatg cttccaggct
      661 ccaattccaa cgctcgctaa aatcaccttt taaacccagg gtgaaatgat ccgcctgggg
      721 ggattttaaa tcggggttgc ccactaacgg catgactgta tctaaatccg gaaagcgatc
      781 ataacggcct gccgatgcgg tgagcgccag ttgctttacc atttgccagg ttaatgccgc
      841 gcgggggtgg agaaagtttt cctcggtaaa atcgttgtgg ttctgttgca ggcctaaatc
      901 aagattaaat gcatccgtca tttgccaatt atccgtgagg tacagcattc gataattcac
      961 atcgacagat cggctgcctt cgataatacc gcgtcggttg ccatcacaat ccggttcaaa
     1021 atccgtacac acaatattca ccatgcggat gcggtagtta taggcgtagc gatttaattc
     1081 actgccgagg gtgagcctgt ggttgccaaa aggttggctg acggctgcgc gcaagaggga
     1141 attggtaaaa tccgtgcgga aaaaataacc ctcgccccaa ctgatggtgt cggtattatt
     1201 attttgcgcc accgccaatt gcgcagtgcc acccgaggca aaattgtgtt gccagcggcc
     1261 cccttgagta tcaaagccct tatcaatttt cgcccgcccg gcaaaatctg ggttgcgggc
     1321 ggtgaaattg ttggtctcgg ataaatccgc ctcaatgcca tcattggcgc cagcaagcgt
     1381 cagagtgatt tgatcttgag ggcttagatc ccatacatat ttaaattgat agtcgtcatc
     1441 cgacggcggc gattcaatgc gcacgccatc ttcatcgggt ttatcatctt tgcttaataa
     1501 atattgcaac atgccctgcc gcagggataa ataaaatgcc gagtgctccg ttgctgcgcc
     1561 ttctacaaat agtcccgccc gcaaaaagga taaattaacc gttgtggata aatcctggtg
     1621 gcgcgggtcc cgcaggcgta tatcaaaaat agccccggtg gcattggagt agggcgcgcc
     1681 aaaacctgcg gaaaataatt gaaaatcctg aataacgtgt tcatccagaa tcgacgtatt
     1741 aaaatcgtga aaaatataac tggcgggcag gccatctatg taatagcggt tatcgctggg
     1801 ggaagagccg cgcaccgcag gcgccccgcc ttgcgcgggc gtcataacac ccggcagggc
     1861 ggtaatagcc cccagcgggt cccctaagga gccgggcata tttaccagtt tctcggtatt
     1921 ttcggtaatt tccgtataag agcctttgcg gctacctaac accaacactt cttctacagt
     1981 attgtcagcc tgcggtttag cgggtgtagt ggcgtttgcc tggtgggcca taggcagtgc
     2041 taaagcgatg gccaaacggt gcagcataaa tttcatagtg agttcctagt gtgagtcgat
     2101 tcctataccc aaacggcaat tagcgccttt ggggcctaaa agtctgaatt gaatggctga
     2161 attagaaagc tagagtattt aaacaaaata ttcgcggtga agtcgtaaca gagcggggca
     2221 gcttgtatcg gtttgtaaca gaatgtttca gagggttttt gtgggttgtt cgtaatcgat
     2281 gggatagcct ctgttcaacc gcttaaacca tcgctcgcct taatatttaa caccgctcga
     2341 aattgggtcc aattacacta aaaatgtgtg aatacttacc ctctgattaa gtctgaaggt
     2401 gggaatattt tgcaaccatt ccgggttggc cagcctgcgg cctattaacc cggcggcttt
     2461 tatgccgggc taactttttt tgtcaggctt tgggagcggc aggatgtcaa gcctgcggcg
     2521 caagctgccg cgagctgcgc taaaaccata aatccacgca ttttcgcaag tctcagcaga
     2581 gcgagggcaa aatagatcag gacgatctat tttgccgccg ggttaataag ccacacaggg
     2641 cagagcttat gggtttgcaa aaatggtcag gaatttaaat ttaatcaaag catctctaat
     2701 agccatataa ataatatttt atatttgctg cgaaaaatca ccgcacaaat aaatgcacca
     2761 gctggtgtac actgccgagc gttttgccca tggctgcggc tgtttttatt atgaataatc
     2821 tgtcggattg gtttatattt ttgcctttta tcacattggg tttagtcagc agtgcccatt
     2881 gtgtcggtat gtgcggcggc ataatggcgg cgcttacggt gtcaattaca cagcctgcgc
     2941 aatccaaacg ctggttatta ttagtggcct acaatatcgg ccgcattggt agctatggcc
     3001 tgatggggtt tgtattggcc ctgtttagcc agttatttca ccattgggcg ggcggtattg
     3061 tagtgcgttt aattgccggt gctttaatga ttgcgatggg attgtattta gccaatattt
     3121 ggcgcggcct tatacacctt gagcgggtgg ggcgatattt atgggtgtat ttacaaccct
     3181 taagcaaata tttgctccct gtggacaagg gaataaaagc cctcgcccta ggtgcactct
     3241 ggggctggct gccctgcggg cttatttatt cagccttggc actttccctc acccaacctt
     3301 cagcggctct agcgggtgcg gctatgctcg catttggcat gggtacattg cctgcagttt
     3361 taatggtagg tattaccgcg caccatttat cccgtctgtt gcaaacccgt agtgtgcgtt
     3421 tggggctggg tgggctgatg atgctttacg gtatttggac catgagcgcg agtattattt
     3481 atggcgggca tcagcatggc gcgcagatcg aacaaccatc ttcactgaac atcgagattc
     3541 agcaccgtca ccatcgcgaa cctaattaag tgctttaatt cgtccgcggg ttgaagctct
     3601 gccttggaaa ataccctctc catctcgagg tcttcaattt ttatttcaat tgttttaaaa
     3661 ataagttcag ctattcataa ataatgtatc tggcctgatt aaagctgagt ttgtgattat
     3721 tgtgcctatt tacaaacatg taccagtagc tgcttacaag cgtgtaccag tagctgcagt
     3781 tgcgcacgtt agagttgcgc aataatccca ctccagaatc aagcagatac gcctatctct
     3841 tcgctggcaa tttttgcaaa cctgctaaag ggaaaataaa aggtattaat accctcagta
     3901 ttgcccattt atacccagac agccgagata ttaagcccca tcatctcttt taagccgcta
     3961 gcagcgtgtc gcaactacct tgccagccct tcgctgctga cgcatgttaa taagctgcta
     4021 ttaacatggc ggcaaaatag atcgtcctga tctattttgc cgccgctgcg ttaaacttac
     4081 gaaaatgcgt agattttctt gttttaacgc cgcttgcggt gactcgcacc gccgagtgcg
     4141 gcatccttgt cgcctttatt attccggcac taaacgccgc cgggttaata atgccgcctt
     4201 ttcagtttgt ttctgcttac taatttaacc ggcggttaaa gcccctggcc gccggaggct
     4261 tctatccttt gcgcattaat ccatccagtt tcatcatcca atagtgctgc ggcggcgcca
     4321 ccaatatcgt ccggtaatcc cgctcgcccc agggcggtta tttcggcaat gtgttggtta
     4381 agctctttat tatcgcgcac agcgccgcca ccaaaatccg tttcaatggc gccgggggcg
     4441 agggtattta cgcggatgcg gcggggtgct aattctttgg cgaggtaacg agtcaaagct
     4501 tcaacggcag ttttcataat ggcgtaggcg gaatagccag gcaccgtcat acgggttaag
     4561 ccggaggaaa tatttaaaat acgccctgcg tcattgatca gcggcagcaa tgcttgtgat
     4621 aaaaaataag gtcctttaat gtgttcgttc accatgcggt caaattcttc cggtgtggtg
     4681 tgctcaaagg gtttaaaaat ccctgagcct gcattattaa ttaaataatc aaaatggttt
     4741 ttaccccaat ggcttttgag cgcggctttt aattggtccg caaatgcgct aaagccgctg
     4801 ctttggctta tatccaattg cagggcgagg gcttttacgc ctaattggga taattctgcc
     4861 agggttgctt gggcctgttg ctcgttactg tggtaggtga aaataatatc gcggccgcgc
     4921 ttggcaaggt gtatcgccat attcttgcca agcccacggc tgccgccggt aattaatgca
     4981 atttttgtgt tctgcatatg aagtctccac tgttgatact gggtgagggg ttaagttggt
     5041 gaatcagtgg gcttagatta ctgcccgcac cttgcccggg ctatgcccga tattccacat
     5101 tgcttgccta atcctctctt gggtcgctaa ggcccctagt aaaaaccctg ccgccgcgat
     5161 agatttagcc tgttttccta taggtttatt gccatgcgcc ttgatcactt tcaaacccta
     5221 ggccccatac ttgaacaaaa ctccagccaa tggggtataa ctccgacgca ggtgccgggt
     5281 gttgcgatat ttaaagccaa ttccaccact acgccgctgc cgctggtgta tcagcccagc
     5341 ctgtgtatat tggtacaggg ggaaaaacat atagtggttg gcgacgagac ctaccgctac
     5401 cagccggggc aattgctagc ggtcaccctc gatttaccct tgcacagtca aatcacggca
     5461 gcaagtgaag caagccccta tcttttatta aaaatagaat tggacgcggc attaattgcc
     5521 gagctttatc catttgtaca catggaaaaa ttccaagagc agccttatgc caaggggctt
     5581 tttatcggcg atctaaacga agcaaccatg gacgcagtgg tgcgtttggc cgaattaatt
     5641 gccaacccac aacatatagc tgtacttgct aatcaaacca agcgagagat tttttatcgg
     5701 ctcttgtgcg ggccacaggg cgcgttaatt gcccaaatcg ccgtgcaaga ctcttatttg
     5761 cagcgtattg ctaaagcctt gcagttcata aaagaagatt ttaaggcagc ccttagtgtg
     5821 ccctatttgg cgcagttggc gggtatgagt gtatcgtcct tccatgccca ttttaaaaac
     5881 atcaccggca taagcccatt gcaataccaa aaatccttaa ggttaatggc agcgcgcaat
     5941 ttaatgctgg cggaagatat ggacgctgcg gccacggcct accgcgtcgg ctatgaaagc
     6001 ccatcccaat ttagccgtga atatgcgcgg atgtttggca gcccgccggg ccgggatgtg
     6061 cagcaattaa agcaggttaa aaaattgcgg gcgatgtagg tattgagggt aatctgtatg
     6121 cagggtgaca gaccgctaac caaattatgt tcgtttgccg ctacgcaaat gaaagttatt
     6181 gaatttaaag aattggctag ctgaaaggac gatgcattcg acgggagata tccaacccga
     6241 tatgacccag catgtattca gtggttattg gttgaggcct tccgaatccc cttaatccac
     6301 tgattagtct gttttaattt ttcttcctta atctagccaa ttggttagcc agctttaacg
     6361 ccgcctcgat gatatgcgac taatgcatta gcgtggccat ggccgatttt atactctgat
     6421 ttaaggtgcg caacctgctc catgtgtttt ttatcctgta gagaatccaa tacatctagc
     6481 caaaaactta tgggtttacc gtaggttttt tcgattgacg ggaaatacga ggcggggcct
     6541 ttgactttgc tgtcgtctgc catgggatct ccggtttaga ctgggaagtt tgcgaattag
     6601 tgagccaaat tatgcttggt acattaaatt tattttggca aaggttaaat cgagtgcaca
     6661 gtttaaaaat ttgattggtg tcgccttcga gtcgtgagca aaaaagaccg agcaattagc
     6721 caattgctcg gtcttcacaa cgccctaaaa aattaaaccc aatgcagctc cagccctaat
     6781 tcctgcgcac gggcggcatg ggttttgtcg gtgactatag ccgtatgagc caattggggt
     6841 tttaagtagc gctcgcacac ccgttgtaag tccgttaatt gagtagctag caggcggtcc
     6901 ctaaaggcct ggcgtttttc taaggtgcgg ccgtgtaggt cggcgtggaa taagcgcttg
     6961 gcgcggccgg cgggggagtc aggtttgtcg ataccgctga cggcgccgag gatggattct
     7021 tcaatgctct gccagctgtg ctggctgtgc ataaaccagt cgatggcttt atcaaagtcc
     7081 gccagggttt cttccaaacg cgggtcccgg taggaataaa agcggaaggc gccggagttg
     7141 ctgtcttggc tggcgccgcc gccataggcg ccgccctgtt cgcgtatggc gcggtgtaaa
     7201 aagccgttgc gcaataaatt gcccagcacc actagcggcg cgctgtcttc atggtccata
     7261 ggcactgtgg cgtaggcgcg ggcgcagaag tgtacttggc tgttggtgag ccaggcctgt
     7321 ttgatggggg cctgataggg ggcgagggca aagggggtaa agcctgcgcc atcggcactt
     7381 ttgaaggtgt ttttaagtac cttgatgtga tcgtccgctt gctgggcttc ccccacc
//
LOCUS       DAOQGS010000159         7358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4921_length_7358_cov_3.950431, whole genome shotgun sequence.
ACCESSION   DAOQGS010000159 DAOQGS010000000
VERSION     DAOQGS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_7358_cov_3.950431"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PKZ52_07730"
     CDS             <1..1250
                     /locus_tag="PKZ52_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNG59702.1"
                     /translation="SLSQMPEHYVGKIIVLAQSLHSVHWPSLSVACVTLAVMLLWPKF
                     KTPVPPHLPALFIGTLLALGLNSAGLAVDTVQSRFSYSLANGTQGQGIPAILPSFIWP
                     WQQAGADGGRLELSWQLMADLLPAAFAIAMLGAIESLLCAVVMDNMTGKRHSANSEIL
                     GQGIGNIVAPFFGGITATAAIARSAANFRAGAQTPVAGMVHALVVLLGLVGLAPVLGF
                     LPMPAMAALLIVVAWNMSEAPKSVHLIANSGASDRWVFACCFLLTILFDMVVAITAGI
                     VLAAILFMKEMAAATRLIASTEPLTLQGEALPTFWRVFVIHGPLFFAAADRIFSELTL
                     QSIPNSGVIIQLERVTLLDAGGIAALRKWMEFCQKNHIKILLLNPSPQVQTALERAHI
                     GAIPNEFMLAQELSQTPLSFFNL"
     gene            1550..2929
                     /locus_tag="PKZ52_07735"
     CDS             1550..2929
                     /locus_tag="PKZ52_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNG59703.1"
                     /translation="MQVTSAAQSKGLWGMSRPLLVEQGLQLSVPLLDTFFLSRVSDSA
                     AAAVGALTPVLFFGVNILWVTIFSGSSIASQRLGAGNRDKAIATIATYTSWILIVGAL
                     LAVLLYAVSPWVCSLMKLDGPTRAYGIQYMQIICLLMVIWSFKLVFQSILNIFGLPQW
                     NMVANIVFFICNLIGTAFAVYGGFGYEPMGLPGVAWANVIASTIGVCVSAYFVFSRIS
                     LRLPRQVIITEFKPASRHTLRIALPSMLEPLSFDLNMMVLNGFAASLGTAALAAKVYT
                     FNTFLIGLVITLALTLATEVLICQHVGAGNYTKAITQMRQSLKAALWGSGIVVLILLA
                     LNHPIMSLYTSDPWIVGASFWFFLLAAISEPPRAINVMVGGVLRATGDGLLISIVGPL
                     FTWLVAVPAAYFMAFILGWGIFGILLSAVLDEGCRSYFYWRRWRTERWQHTHVHALEA
                     KAEQAQQPV"
     gene            3059..4708
                     /locus_tag="PKZ52_07740"
     CDS             3059..4708
                     /locus_tag="PKZ52_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNG59704.1"
                     /translation="MSQVSERSPSPLKRYNTANEMVEDVFATYAERPAFTCAGQRHTY
                     RDIDRLARQFAVYLQRHLGLVPGDVIALQLPNITQYPVAVYGAIRAGLVIVNINPLYT
                     PHELEHQLVDSGAKVLVVLANVAAAAAQVVPNTPVHTVIVTEFADLMPWPKRPLINFI
                     VRHVKKLVPAVHFAQQVNFNQALSLGLQGELTPVQRTPEDILCLQYTGGTTGRAKGAM
                     LSHFNVCSNSWQVLERNPHLSAAACEVFATILPLYHIYAFCLHVFGGFSIGAHNVLIP
                     NPRDIPAVVKAFAHTPPTVVIALNTLFKALSLNEQFQRLDFSRLHATTSGGMALTHDA
                     AIAWKNITGCEICAGYGLTETSPVVSANVPASNQLTTVGPPLIETQVKLVDSDGNEVP
                     PGHEGELCVKGPQVMLGYWQQPEETAKVMLPGGWLKTGDIAEIQPDGHIKIVDRKKDM
                     ILVSGFNVYPNELEDVLSSYPGVLEAAVIGVPDDKSGEAVKAYLVMKEPCSEEEIRKH
                     CKQFLTGYKIPKYIEFRQTLPKSNVGKILRRELRSEIKSAA"
     gene            4945..6126
                     /locus_tag="PKZ52_07745"
     CDS             4945..6126
                     /locus_tag="PKZ52_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNG59705.1"
                     /translation="MPVNALILSGGGARAAYQVGVLQAFATLLPHLQNPFPIICGTSA
                     GAINALYLAAYLGEFNQAAQDLAAIWRKLHIQQVYHCNWQDLIFGLARVGASLFNRGK
                     GVNKPLALLDNSPLRELLGKLVQFDNLNVRIKRGELEAVSVTALGYHSGDSVTFFQGA
                     PHLQGWRRARRVGQIASLTVEHLLASSAIPTVFPAVFLEREYFGDGAMRQMAPISPAL
                     HLGADRIVVIGVSGNRNPENFVRKNSGGRSSPSMGNIVGQMLNSAFIDALEGDLEHLE
                     RVNHLVRLLPPDILNAAPNYLRPVETLVISPSKPLDKIAGRHVRDLPPSMRFFMRATG
                     STASSGGASAASYLLFSNEYCHELIELGYQDAMWEKDNIVRFFADQACPVQPAKKPSK
                     K"
     gene            6178..7038
                     /locus_tag="PKZ52_07750"
     CDS             6178..7038
                     /locus_tag="PKZ52_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNG59706.1"
                     /translation="MLRLIPVFVLLCTSVLWGASWLPLKALHGRGADGLALIACAYGV
                     LAIMALPWVWRARSSIRGHWHWLLAIVLLGGAANVSFSYAIIYGDVVRVMVLFYLLPV
                     WGVLGGRFILKEPTAWWRWVGVAMALAGAFIILGGPNILARPINYLDFIAIGSGIFFA
                     ASNLVFRGVEQVRLPPKLFALFLGSSLLAHLLLTPAQSWAQAQFPLGWVLLYATTWLL
                     LANWGSLWAITQMPAGRSAIILIMELITAVVTQVAFTEQTLQGYEWIGGSLIMAAALT
                     EIIAAQRSKE"
BASE COUNT         1515 a         1760 c         2004 g         2079 t
ORIGIN      
        1 taagcttaag ccaaatgccc gaacattatg taggtaaaat tattgtgcta gcgcaatcgc
       61 tgcacagtgt gcactggcct agcctgagtg tggcctgtgt gaccttggcc gttatgctgc
      121 tgtggccgaa atttaaaacg ccggtaccgc cgcatttacc ggcattattc atcggtacgc
      181 tactggcact gggtttaaat tccgcgggtt tagcggtgga tacggtgcaa tcgcggttta
      241 gttatagtct cgccaatggc acccaggggc agggtattcc cgctattttg ccgagtttta
      301 tttggccctg gcagcaagcg ggggcagatg gtgggcgctt ggaattaagc tggcaattaa
      361 tggccgattt attgccggcc gcgtttgcca ttgccatgct cggcgcgatt gaatcgctgt
      421 tgtgtgccgt ggtaatggat aatatgaccg gcaaacgcca cagcgccaac tcagaaattc
      481 ttggccaggg cataggcaat atagtggcgc cattttttgg cgggattacc gccacggccg
      541 ctattgcccg ctcggcggct aattttcgcg ccggtgcgca aacgccggtg gcgggtatgg
      601 tacacgctct agtggtgtta ttaggcttgg taggtttggc acctgttttg gggtttttgc
      661 ccatgcccgc catggcggct ttattaattg tggtggcgtg gaatatgagt gaagcgccta
      721 aaagtgtgca tttaattgcc aacagcggcg cgtcggatcg ctgggtgttt gcctgttgtt
      781 ttttgctaac cattttattt gatatggtgg tggcgattac tgctggaatt gtgcttgccg
      841 cgattttgtt tatgaaagaa atggccgcag ccacgcgatt aattgccagc acagagccac
      901 ttactctgca gggtgaagcg ctgcccacat tctggcgcgt ttttgtgatt cacgggccat
      961 tattttttgc cgccgccgat cgcatcttca gtgaattaac cctgcagagc atccccaata
     1021 gcggcgtcat tattcaatta gagcgcgtca ccctgttgga tgcaggcggt attgcggcgc
     1081 tgcgcaaatg gatggaattt tgccaaaaaa accatatcaa aattctccta ctgaatccct
     1141 cgccgcaggt tcaaacagca cttgagcgcg cgcatatagg tgctattccc aatgaattta
     1201 tgctggccca agagctttca caaacgcctt tgagtttttt taatctttag cctgccaagc
     1261 actttagaag tctacctgca gcaacagagt ttgctgcggc gggtagttac tttacgcaga
     1321 tgcctgttaa tgtttttggg gaattttttt gggaataata tttcgcggcg ccatagtgtc
     1381 atttctaaaa taagattaat cctaagcgca agagcctaat tttattcttg gcgttgcagc
     1441 cgcagggcgc cgttcgtctg tacccaaaaa ataaatgcgc cgcgcgcccc tcttgctgcg
     1501 caagacctct ataatgcgcg gcttttgcgt gtgcagtagt gagagatata tgcaagtaac
     1561 cagtgcggcc cagtctaaag gcctgtgggg catgtcgcgg ccattgttgg tggagcaggg
     1621 gctgcagctg agcgtgcctt tattggatac tttttttctc agccgcgtga gtgattcagc
     1681 tgcggcagca gtgggcgcct taacaccggt tttatttttt ggcgtcaata ttttgtgggt
     1741 gacgatattt tcaggttcta gtattgccag ccagcggtta ggggcgggca atcgggataa
     1801 ggctatcgcc actattgcca cctacaccag ctggatttta atagttggcg ccctgttggc
     1861 ggttctttta tatgccgtct ccccctgggt gtgcagcctg atgaaactgg atggccccac
     1921 acgcgcctac ggcattcaat atatgcagat tatttgtctg ctgatggtga tttggtcatt
     1981 taaattagtg tttcaaagta ttttgaatat atttggcctg ccgcagtgga atatggtggc
     2041 caatattgtg ttttttatat gcaatttaat cggcaccgcg tttgccgtgt atggcggttt
     2101 tggctatgag cctatgggcc tgcctggtgt ggcctgggcc aatgttatcg cttcaacaat
     2161 cggcgtttgc gtcagtgcgt attttgtctt tagccgcatt tcgttgcggc tgccgcggca
     2221 ggtcatcatc actgaattta aacccgcctc ccgccatacc ctgcgcattg ccctgccgag
     2281 tatgctggag ccgctgtcgt ttgatttaaa tatgatggtt ttaaatggct ttgcggccag
     2341 cttaggcacc gctgcgctgg cggcaaaagt ttatacattt aatacttttt taattggttt
     2401 ggtgattacc ctcgcgctaa cactcgccac cgaagtatta atttgccagc atgtgggggc
     2461 gggcaattat accaaggcga ttactcaaat gcgccaaagc ctaaaagcgg cgctgtgggg
     2521 ttcaggtatt gtagtgttga ttttgctggc ccttaatcac ccgattatgt cgttgtacac
     2581 cagcgacccg tggattgtcg gcgcgtcctt ttggtttttt ttattggcgg ctatttcaga
     2641 gccgccacgg gcgattaatg taatggtggg tggtgtgttg cgcgccaccg gggatggttt
     2701 attaatttct attgtcggcc ctttatttac ctggctggtg gcggtgcctg ccgcctactt
     2761 tatggcgttt attttgggct ggggtatttt tggaattttg ttgtctgcgg tgttagatga
     2821 gggctgccgc tcttattttt attggcgccg ctggcgcact gagcgctggc agcacaccca
     2881 tgtccacgct ctggaagcca aggccgagca ggcgcagcag ccggtttagc ctgcggccct
     2941 ggcctgcccg gcgggggttt ggcgatgaat tcgcaggcgg caagtgggaa ttacttgggc
     3001 aagggactaa actgcagtta gcatactgcc ttcattcctg tttaagtgcc gcgtaattat
     3061 gtcccaagtg tcagaacgct cgcccagtcc ccttaaacgc tataacacgg ctaatgaaat
     3121 ggtggaggat gtgtttgcca cctatgccga gcgcccggct tttacctgtg cgggccagcg
     3181 ccacacctat agggatatag atcgcctcgc gcggcaattt gccgtttatt tacagcgtca
     3241 tttgggccta gtgcccggcg atgttattgc cctgcaatta cccaatatca cccaataccc
     3301 tgtggccgtg tacggcgcca ttcgcgcagg tttggtgatt gtgaatatta acccgctcta
     3361 taccccccat gaattggagc atcaattggt ggattccggc gctaaggtgc tggtggtgtt
     3421 ggcaaacgtg gcggctgcgg ctgcccaggt ggtgcccaat acacccgtgc ataccgtaat
     3481 tgttaccgaa tttgccgatt taatgccctg gcccaaacgg ccactcatta attttattgt
     3541 gcggcacgta aaaaaattag tgcccgccgt gcattttgcc cagcaggtta attttaatca
     3601 ggcgctcagc ttgggtttac agggtgaatt gacacccgtg cagcgaacgc ctgaggatat
     3661 tttatgtttg caatataccg gcggtaccac aggccgcgcc aaaggcgcta tgttgagcca
     3721 ttttaatgtc tgctctaaca gctggcaggt gctggagcgc aatccgcatt tatcggcggc
     3781 ggcctgtgaa gtatttgcca ctattttgcc gctgtaccat atctatgcgt tttgtttgca
     3841 tgtgtttggc ggcttttcaa tcggcgcaca caatgtatta attcccaatc cgagggatat
     3901 tcccgccgtg gttaaagcct ttgcccatac accgcccaca gtggtcatcg cgttaaatac
     3961 cttatttaaa gccttaagcc tcaatgagca attccagcgc ttggattttt cccgtttaca
     4021 cgccaccacc tccggcggta tggcgctgac ccacgatgcc gccatcgcct ggaaaaatat
     4081 taccggctgt gaaatatgtg caggctatgg ccttaccgaa acctcgccgg tggtgagcgc
     4141 taatgtaccc gccagcaatc aattaaccac cgttggcccg cccttaattg aaacccaagt
     4201 taaattagtg gatagcgacg gcaatgaggt gccgccgggg cacgagggtg agctgtgtgt
     4261 aaaaggcccg caggtgatgt tgggctattg gcaacagccg gaggaaaccg ccaaagtcat
     4321 gttgccgggc ggctggttaa aaactggcga tatcgcggaa attcagcccg atgggcatat
     4381 taaaattgtc gatcgcaaaa aagatatgat tttggtctcc ggctttaatg tgtatcccaa
     4441 tgaattagaa gatgtgctca gcagttaccc aggtgtatta gaggcagcgg ttatcggtgt
     4501 gcccgatgat aaatccggtg aggccgtaaa ggcttatttg gtgatgaaag agccctgttc
     4561 agaagaagaa atacgcaaac actgtaagca gtttttaacc ggctacaaaa ttcccaaata
     4621 tattgaattc aggcaaacac tgcccaagtc caatgtgggc aaaatattgc gccgggaact
     4681 gcgcagcgag attaaatccg ccgcttaaac aaacaggttg ttgtttaatg gcaattggct
     4741 tcgaggtaga catgggcgca accatcggcg ctgtattttc cgttggcggg gcttttggcg
     4801 tgcgggttaa tggctgtgcg gaagccatga atacacatca tttcagaccg tcaaaaagag
     4861 gaaccttttt gacgagccta cagggatgta ttcacggcgt gtctgaaatg atgtgtattc
     4921 attgctggtg cctaggtagt aattatgccg gttaatgcgc tcattttatc cggcggtggc
     4981 gcccgcgccg cctatcaagt gggtgtattg caggcctttg ccaccctttt gccccatttg
     5041 caaaatcctt ttcccattat ttgcggcact tctgcaggcg ccataaatgc gttgtattta
     5101 gccgcttatt taggtgagtt taatcaggcc gcccaggatt tggcggctat ttggcgcaaa
     5161 ttacatattc aacaggtgta tcactgcaac tggcaggatt taatatttgg cctcgctcga
     5221 gtgggggcgt ctttatttaa tcgcggcaag ggcgtcaata aacccttggc cttattggat
     5281 aacagcccgc tgcgggaatt gctcggcaaa ctggtgcaat ttgacaacct caatgtgcgc
     5341 attaaacgcg gtgaattaga agccgtttcg gtgacggcct taggttatca ctccggcgat
     5401 tcggtgacgt tttttcaggg cgccccgcat ttgcaaggtt ggcgcagggc gcgtcgggtt
     5461 gggcaaattg ccagtttaac ggtggagcat ttgcttgcct catcagctat tcccacggta
     5521 tttccggcgg tttttttaga gcgggaatat tttggcgatg gcgctatgcg gcagatggcg
     5581 cctattagcc cggccctgca tttaggggcc gatcggattg tggtgatcgg tgtcagcggc
     5641 aatcgcaacc cggagaattt tgtacgcaaa aatagcggcg gccgctcctc gccttcgatg
     5701 ggtaatattg tcggccagat gctcaatagc gcctttattg atgcgctgga aggcgatttg
     5761 gagcatttag agcgagtcaa tcatttagtg cgcctattgc cgccggatat attaaatgcc
     5821 gcgcccaatt atttgcgccc ggttgagact ttagtgattt ccccgtccaa acccctggat
     5881 aaaattgccg gccgccatgt gcgggatctg ccgccctcta tgcgcttttt tatgcgcgcc
     5941 accggctcca cggccagtag cggcggcgcc tcggcggcca gttatttatt attttctaat
     6001 gaatattgcc atgaattaat tgaactgggc tatcaagatg ctatgtggga gaaggataat
     6061 atagtgcgct tttttgccga tcaggcctgc cccgttcaac ctgcgaaaaa accgtccaaa
     6121 aaatagcgcc caggttatcg cctcgcgcca cctgcacatt tagtctggtc gtacatcatg
     6181 cttaggttaa ttcccgtatt tgttttgctt tgcacttctg tgttgtgggg tgcctcttgg
     6241 ctgccattaa aagccctaca cgggcgcggc gctgatggtt tggcgctaat tgcctgcgct
     6301 tatggagtgc tggcgattat ggcgctgccc tgggtgtggc gggcgcgcag cagtatccgc
     6361 ggccactggc attggctgct ggccattgtg ttgttgggcg gcgctgccaa tgtgagtttt
     6421 tcctacgcca ttatttatgg tgatgtagtg cgggtgatgg tgctgtttta tttattgccc
     6481 gtgtggggtg ttttgggtgg gcgttttatt ttaaaagagc ccaccgcctg gtggcgctgg
     6541 gtgggtgtgg caatggcgct ggccggtgct tttattattc ttggcgggcc gaatattttg
     6601 gcaaggccga ttaattattt ggattttatt gcgattggtt cagggatttt ttttgccgcc
     6661 agcaatttag tgtttcgcgg cgtggagcag gtgcgcctgc cgcctaaact atttgccttg
     6721 tttttaggct caagcctgct tgcgcacctt ttattaaccc ctgcgcagtc gtgggcgcag
     6781 gcgcaatttc ctttaggctg ggtgctgctg tacgccacta cctggttgct cctcgccaat
     6841 tggggctcac tctgggcaat cacccaaatg cctgccggtc gctcggcgat tattttaatc
     6901 atggaattaa ttactgctgt ggtcacccag gtggctttta ccgaacagac gctgcagggt
     6961 tatgagtgga ttggcggcag tttaattatg gctgctgcgc tgacagaaat tattgccgcc
     7021 cagcgcagta aagaatagtg gcaaattacc tctttagctc gctgtatctc gttcccacgc
     7081 tctgcgtggg aatgcatatt tggcacctgt tgcgtatcgc cccggcaaag ctcacctgca
     7141 gcaataaatg caggttattg cagacatgcc gtaaatacat ccgtgcaggc cgtcaaaaag
     7201 attcctctat tgggcggccc gcaattaggt gcatttgttg ccgctgcacc gcacttcatg
     7261 gtaaggcaat ttcattaagg taaggcaatt tcattaaggt aaggaattag tgctgttttt
     7321 acggttgttt tcgcttatcc gccaacccca ccccagcc
//
LOCUS       DAOQGS010000160         7334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4942_length_7334_cov_4.081879, whole genome shotgun sequence.
ACCESSION   DAOQGS010000160 DAOQGS010000000
VERSION     DAOQGS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_7334_cov_4.081879"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..555
                     /gene="rimP"
                     /locus_tag="PKZ52_07755"
     CDS             100..555
                     /gene="rimP"
                     /locus_tag="PKZ52_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNG59707.1"
                     /translation="MASLQERLEALLEPVVEALGCQLWGIEFFAQGRRSVLRIYIDKP
                     DGIGVEDCEQVSRQASAVLDVEDPISSEYTLEVSSPGMDRPLYKLSQYEQCVGENLIV
                     KLRVAFEGRRKFSGLLKGVEGDEIVLQVDDEEYLLPFELIDKANVAPRF"
     gene            578..2092
                     /gene="nusA"
                     /locus_tag="PKZ52_07760"
     CDS             578..2092
                     /gene="nusA"
                     /locus_tag="PKZ52_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNG59708.1"
                     /translation="MKKEIVPVAKKEILLVADAVSNEKGVDRNIIFEAIELALATAAK
                     KRFDEDSTITVTIDRRTGGYVTERSWKVVGDDTLAALGTEFTLQEAHEKDPNLKPGDV
                     YSEIIENVEFGRIAAQTAKQVIVQKVREAERAQIVDEYRGKVGELVSGMVKKVTRDNI
                     IVDLGNNAEGLLPRDQLVGREIFRMNDRIRAVLLEVRPDARGPQLFLSRSCPQMLLEL
                     FRIEVPEIAEEIITLRGAARDPGSRAKIAVSTNDGRIDPVGACVGMRGSRVQAVSNEL
                     AGERIDIVLWHDNPAQYVINAMAPAEIDSIVIDEDTGAMDVAVADDGLAQAIGRGGQN
                     VRLASELTGWEINVMSMAKWQEKQAAETGSVKETFMAALDIEEDVAEVLVEEGFTTLE
                     EIAYVPLEEFLAIEGFDEEIAQELRARAKDALLTQALASEEELENTDPAEDLLTMEGM
                     DRPLAFVLASIGIRTMEDLAEQSVDDLLDIEGMTEKRAASLIMAARAPWFADSK"
     gene            2111..4810
                     /gene="infB"
                     /locus_tag="PKZ52_07765"
     CDS             2111..4810
                     /gene="infB"
                     /locus_tag="PKZ52_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNG59709.1"
                     /translation="MADVTVNDLAKSVGASVERLLSQMGQAGLAHKKATDMVSDEEKQ
                     TLLAFLKTQHGESAEAPRKITLKRKTTTTIKTGTGSARKTVNVEVRKKRTYVKRTDLE
                     GDAGGDQDLLQDAEELLEIDNEQATFEAVEAEVPAPVAEIAAPEVSEEAPQATEAEAA
                     PAQPRVFVDDAENRRQAAILARQNATKEADALARKAAEERRKKDEAKKLVTKKPTAAP
                     VLSAEELAAKAEEEKHKLSKKKERTIEIPIEEEEDSRNRHNKKAGKAVKKTAVVAPKK
                     GSAVLTYLDEDNEFGGPRGHRKGGRKLANKHGFKKPTSKIVHEVEIPESITVSELASR
                     MSVKGTEVVKHLMKMGVMASLNQVVDQDTAHIVVADFGHQAVLVSHDAVEHNFQRTID
                     EQDGEYVTRAPIITVMGHVDHGKTSLLDYIRKAKVAAGEAGGITQHIGAYRVPTSHGE
                     LTFLDTPGHAAFTAMRARGARCTDVVILVVAADDGVMPQTEEAIKHAKAAGVPMVVAI
                     NKCDKPSADPDRVKNELVVKDVIPDDWGGDTQFVQVSALTGDGVDQLLEAVAIQAELL
                     ELNAQINVPGRGVVIESRMDKGRGVVATLLVQNGELKTGDMVLAGQSFGRVRAMVDEH
                     SAQIKAAGPSMPVEILGLDVAPNAGDEFAVVSDERKAREVSEFRAVKEREERMARTQV
                     SKLENMFANIAAGEKKTLSVVVKTDVRGSLEAINAALLELGNSEVEVRIVGDGVGGIT
                     ENDINLAMTTRAIVVGFNVRADGSARKLAEREGVDIRYYSIIYQLIDEVKAALSGMLD
                     PERVETILGIAQVRDTFRSPKFGQVAGCMVLEGTVYRNKRIRVLRDNVVIFEGELESL
                     RRFKDDVAEVRHGMECGIGVKNYEVKVGDQIEVYEVKEVARSL"
     gene            4829..5224
                     /gene="rbfA"
                     /locus_tag="PKZ52_07770"
     CDS             4829..5224
                     /gene="rbfA"
                     /locus_tag="PKZ52_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNG59710.1"
                     /translation="MAREFKRADRVADAVQRSLAQAIPNEVRDPRLGMVNINAVVVAK
                     DLTMAKVYFTLVGEQDAARCRQAVEILNKAASFLRGIVCKDVALRISPRLFFYYDEAS
                     IKGQALSQLIDKAVAADKAHQHDTGSNEE"
     gene            5228..6169
                     /gene="truB"
                     /locus_tag="PKZ52_07775"
     CDS             5228..6169
                     /gene="truB"
                     /locus_tag="PKZ52_07775"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNG59711.1"
                     /translation="MGRSKHYGRVVDGLLILNKPHGITSNRALQQAKHLFFANKAGHT
                     GSLDPLATGVLPLCFGEATKFSSHLLDADKRYQTRIQLGVRTTSCDCEGEEISRVDAS
                     HITLEQLNNALAAFRGPILQVPPMVSALKHQGQPLYKLAREGLEIERAPRPVTIYSLD
                     LLDFTAGPAPQAVLDIHCSKGTYIRSIAADLGDALGVGGHVVQLHRTQAGPFTIAQAV
                     LLETLAAERGEQGPEVLDHHLLALDAAIETLPKLTLEDNSAYYFVKGQGVMDLRVYRL
                     GQQGDRVRVFRSDGRFLGLGEIGDDGLINPKRLVQSV"
     gene            6304..6573
                     /gene="rpsO"
                     /locus_tag="PKZ52_07780"
     CDS             6304..6573
                     /gene="rpsO"
                     /locus_tag="PKZ52_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNG59712.1"
                     /translation="MALSAAEKAAILKEHQTAANDTGSPEVQVALLTANINKLQSHFE
                     GHKQDHHSRRGLIRMVNQRRNLLDYLKSKDSTRYSSLIQKLGLRR"
     gene            6646..>7334
                     /locus_tag="PKZ52_07785"
     CDS             6646..>7334
                     /locus_tag="PKZ52_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNG59713.1"
                     /translation="MNPVIKQFQYGKETVTLETGRIARQATGAVVVSIAQTRVLCTVV
                     GAKQASPGQGFFPLSVHYQEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PEGFLNEVQVVCTVLSVEKDQDPDIAAMIGTSAALAISGIPFNGPIGAARVGYTVKDG
                     YLLNPANSQLLSSELDMVVAGTQNAVLMVESEANELPEDIMLGAVLFAHQEMQAVIQA
                     VNELARDAAKPA"
BASE COUNT         1846 a         2017 c         2028 g         1443 t
ORIGIN      
        1 gttttttgtt atctggcctt tgcaaaggca aaccaaaggt gcggcacctg cggttttttt
       61 aaatgggcct tgagcccatt ttttgtttgt gagtacgtta tggcatcgtt acaagagcgt
      121 ttagaagctc tgttagaacc cgttgttgag gcgctcggct gtcaactctg gggcatcgaa
      181 tttttcgccc agggccgccg ctcggtactg cgcatttaca tcgataaacc cgacggtatc
      241 ggcgttgaag actgcgaaca ggtcagccgc caagccagcg cggtgttgga tgtcgaggac
      301 cctatctcca gcgaatacac cctggaagtt tcatcccccg gcatggaccg cccactgtat
      361 aaattgagtc aatacgagca gtgcgtgggt gaaaatttaa ttgttaaatt acgcgtggcg
      421 tttgaaggcc ggcgcaaatt cagcggcctg ttaaaaggcg tagagggcga tgaaattgtg
      481 ctgcaggtgg acgatgagga atatttatta ccctttgagt taattgataa agcgaatgtt
      541 gccccccgat tctaggaaat aataaagaga gaatttcatg aaaaaagaaa ttgtgccggt
      601 cgctaaaaaa gaaattctgt tggtagccga tgcggtctca aacgagaaag gcgttgaccg
      661 caatattatc tttgaagcca ttgagctggc cctggccacg gccgctaaaa aacgctttga
      721 cgaagactcc acgattaccg tcaccatcga ccggcgcacc ggcggctatg tgactgagcg
      781 cagctggaag gttgtcggcg atgacactct ggcggccctc ggcaccgaat ttactctgca
      841 agaagcccac gagaaagacc ccaatttaaa gcccggcgac gtgtattctg aaatcatcga
      901 aaacgtcgag ttcggccgta tcgccgcgca aaccgcgaaa caagtgatag tgcaaaaagt
      961 gcgggaagca gagcgggcgc agattgttga tgaataccgc ggcaaagttg gcgagctggt
     1021 gtccggcatg gtcaaaaaag ttacccgcga caatattatt gtggacctgg gcaataacgc
     1081 cgaaggcctg ctgccccgcg atcaactggt agggcgggaa atattccgca tgaacgatcg
     1141 catccgcgcg gtactgttag aagtgcgccc cgatgctcgc ggcccacaac tgtttttaag
     1201 ccgctcctgc ccgcagatgt tgctggaatt attccgcatt gaagtgccgg aaattgccga
     1261 agaaattatc accctgcgcg gcgccgcccg cgacccaggc tcccgcgcca aaatcgcggt
     1321 gtccaccaac gatggccgca tcgacccagt cggcgcctgc gtgggtatgc gcggctcgcg
     1381 ggtacaagcg gtatccaatg aactggccgg tgagcgcatc gatatagttt tgtggcacga
     1441 caaccctgct cagtacgtta ttaacgcaat ggctccggcg gaaatcgact ccatcgtgat
     1501 cgacgaagac accggcgcta tggatgttgc ggtggccgat gacggcctcg cccaagccat
     1561 tggccgcggc ggtcaaaacg tgcgcctggc ctctgaactg accggctggg aaatcaacgt
     1621 catgagcatg gctaagtggc aggagaaaca agccgccgaa accggcagcg taaaagaaac
     1681 ctttatggcc gccttggata ttgaagagga cgtggctgaa gtgttggtgg aagaaggctt
     1741 caccacctta gaagaaatcg cctatgtacc tctggaagaa tttttggcca tcgaaggttt
     1801 tgacgaagaa atcgcccagg aactgcgggc ccgcgccaaa gacgcgctgt taacccaagc
     1861 cctggcaagc gaagaagagc tggagaacac cgacccagcc gaagacttat taaccatgga
     1921 gggcatggat agacccctgg cgtttgtcct cgccagcatc ggtattcgca ctatggaaga
     1981 cctagccgaa caatccgtcg acgatctgtt agatatcgaa ggcatgactg aaaaacgcgc
     2041 ggcgagcctg atcatggccg cccgtgcccc ctggtttgca gactctaaat aggtgagctg
     2101 aggaaataca atggcagacg taacagttaa cgacctagcc aaatccgtag gcgcatccgt
     2161 ggagcgtcta ctgtcgcaaa tggggcaagc gggtttagca cacaaaaaag ccacggatat
     2221 ggtatcggac gaagaaaagc aaacgctgct ggcgttttta aaaacccagc atggcgagag
     2281 cgctgaagca ccgagaaaaa ttaccctcaa gcgcaaaacc actaccacca ttaaaaccgg
     2341 caccggcagt gcgcgcaaaa ccgtcaatgt agaagtgcgc aaaaaacgca cctacgtgaa
     2401 acgtaccgat ttagaaggcg acgccggtgg cgaccaagac ctgctgcaag acgcagaaga
     2461 gctgctggaa attgataacg aacaggccac attcgaagcc gtggaggccg aggtgcctgc
     2521 accggttgcc gagatagcag ccccagaggt cagcgaggaa gccccccagg ccactgaagc
     2581 cgaagccgcc ccggcgcaac cgcgcgtatt tgtggacgat gcggagaatc gccgccaagc
     2641 cgctattttg gcccgccaaa acgctacaaa agaagccgac gccctggccc gtaaggccgc
     2701 cgaagagcgc cgcaaaaaag acgaagcgaa aaaactcgtc accaaaaaac ccacggctgc
     2761 cccagtgctc agtgccgagg agttagcggc caaggccgaa gaagaaaaac acaagctcag
     2821 caagaaaaaa gagcgcacta ttgaaatccc gattgaggaa gaggaagaca gccgcaatcg
     2881 ccacaacaaa aaagcgggca aggccgttaa gaaaacagcc gtagtagcgc ccaaaaaagg
     2941 ctccgccgtc ctgacttatt tagatgaaga caatgagttt ggcgggccgc gcggccatcg
     3001 caaaggcggc cgcaagctag ccaacaaaca tggctttaaa aagcctacga gtaaaattgt
     3061 gcacgaagtt gaaatccccg aaagcattac cgtctctgaa ctggcctcgc gcatgagcgt
     3121 gaaaggcacc gaagtggtga aacatctgat gaaaatgggt gttatggcca gcctcaacca
     3181 ggtggtcgac caagacaccg cccacatagt tgtggccgac tttggccacc aggccgtatt
     3241 ggtcagccac gatgcggtgg agcacaactt ccagcgcacc atcgacgaac aggacggcga
     3301 atacgtcaca cgggccccca tcatcacggt gatgggccac gttgaccacg gtaaaacctc
     3361 cctgctggac tacatccgca aggccaaggt ggcggccgga gaagccggcg gtattaccca
     3421 gcatattggc gcctaccgcg tgcccaccag ccacggtgag ctcacctttt tagatacccc
     3481 cggccacgcc gcctttaccg ccatgcgcgc ccgcggcgcc cgctgtactg acgtggtgat
     3541 tctggtggtg gccgccgatg acggcgtaat gccacaaacc gaagaagcca tcaaacacgc
     3601 caaagccgcc ggtgtaccta tggtagtggc gattaacaag tgtgataaac ccagcgctga
     3661 tccagaccgg gtgaaaaacg agttggtggt taaagatgta atccccgatg actggggcgg
     3721 cgatactcaa tttgtgcagg tatcggctct taccggcgat ggcgttgacc aattgttaga
     3781 agctgtggcc atccaagcgg aattgttgga gctgaacgcg caaattaatg tgccgggccg
     3841 tggagtggtg attgaatcgc gcatggataa aggccgcggt gtggttgcca ccctgctggt
     3901 acaaaacggt gagctcaaga ctggcgacat ggtattggcc ggtcaaagtt ttggccgcgt
     3961 gcgtgccatg gttgacgaac acagtgctca aatcaaagcc gctggcccct ctatgccagt
     4021 ggaaatttta ggcttagatg tagcccccaa cgccggtgat gaattcgccg tggtctccga
     4081 tgagcgcaaa gcccgtgagg tgtctgagtt ccgcgccgtg aaagagcgcg aagagcgcat
     4141 ggcgcgcacc caggtttcca aattggaaaa catgtttgcc aatatcgccg ctggtgagaa
     4201 gaaaaccctt tctgtggtgg tgaaaaccga cgtgcgcggc tccttagaag ccattaacgc
     4261 cgctctcttg gaactgggca acagcgaagt ggaagtgcgc attgttggcg acggtgtcgg
     4321 cggtattact gaaaacgaca tcaacctggc catgactacc cgcgccattg tggtgggctt
     4381 taacgtgcgt gccgacggca gcgcccgcaa actggccgaa cgcgaaggcg tggatattcg
     4441 ttattacagc atcatttacc agctgatcga cgaggtaaaa gccgccctct ccggtatgct
     4501 cgaccccgag cgcgtggaga caattctggg tatcgcccag gtgcgcgaca ccttccgctc
     4561 ccccaaattt ggtcaggtgg ctggctgtat ggtgttggaa ggtacggtct accgcaacaa
     4621 acgcatccgt gtgttgcgtg acaacgtggt gatcttcgag ggcgaattgg aatccctgcg
     4681 ccgctttaaa gacgacgtag ccgaagtgcg ccacggtatg gaatgtggta tcggtgtgaa
     4741 gaactacgaa gtgaaagtag gcgaccagat tgaagtctac gaagtgaagg aggtggcgcg
     4801 ctcgctctaa gggcagccgc cgattcctat ggcacgagag tttaaacggg ccgaccgcgt
     4861 cgccgacgcg gtgcagcgca gcctggccca ggcaattccc aacgaagtgc gcgacccgcg
     4921 cttaggcatg gtgaacatca acgctgtggt ggtggctaaa gacctcacca tggccaaggt
     4981 gtacttcacc ctggtgggcg agcaggatgc tgcccgctgc cgtcaggcgg ttgagatact
     5041 gaacaaggcg gcgagcttcc tgcgcggcat cgtctgtaaa gacgtggccc tgcgcatcag
     5101 cccgcgcctg ttcttttatt acgacgaggc ctccataaag ggccaagctc tcagtcagct
     5161 cattgacaaa gcggtggccg ccgataaagc ccaccagcac gacaccggct ccaacgagga
     5221 ataaaccttg ggccgcagta aacattacgg ccgggtggtt gacggcctgc ttattctcaa
     5281 caaaccccac ggcattacct ccaaccgcgc attgcaacag gcaaaacacc tgttcttcgc
     5341 caacaaagca ggccataccg gcagtcttga cccactcgcc accggggttt tacccctgtg
     5401 ttttggcgag gccaccaagt tttcctccca cctattagat gccgataagc gctaccaaac
     5461 ccgtattcaa ctgggcgtgc gcaccaccag ctgcgactgc gaaggggagg aaattagccg
     5521 ggtggatgcc agccatataa ccctagaaca attaaataac gccttggcgg cttttcgcgg
     5581 cccgattctg caggtgccgc ccatggtatc cgccctaaaa catcagggtc agcccctgta
     5641 taagctggcc cgggaagggt tggaaattga gcgtgcgccc cggccggtga ctatttacag
     5701 cctcgactta ttggatttca ccgccgggcc tgcgccacag gcggtgctgg atattcactg
     5761 cagcaagggc acttatattc gctccatcgc tgcagacctg ggggatgcat tgggtgtggg
     5821 cggccatgtg gtgcaactgc accgcaccca ggccgggcca tttaccattg cacaggccgt
     5881 gttgcttgaa accctagccg cagaacgggg cgaacagggg cctgaggtat tggatcatca
     5941 cctgttagca ctggatgcgg ccatagagac cctgccaaaa ttgacgcttg aggacaacag
     6001 cgcctactac tttgtaaagg gtcagggtgt gatggatctg cgggtctacc gcttggggca
     6061 gcaaggtgat agagtgcgcg tctttcgctc ggacggtagg tttttaggcc ttggcgaaat
     6121 tggcgatgac gggttaataa accccaaacg cctcgtccag agtgtttagg gcagctgtta
     6181 cattatccaa cgccttaaac actgcgacct aactgtcaat atgcgcggtt aggccgaact
     6241 tccccgcagc actttaagct gctggggtgc atattcaact taaccgtaaa tgaggaaata
     6301 cccatggcac tgtctgccgc tgaaaaagcc gctattttga aagaacacca aactgccgcc
     6361 aacgatactg gatctccgga agtacaagtg gctcttttaa ctgccaacat caacaaactg
     6421 caatcccact tcgaaggcca taaacaagat caccactctc gccgtggctt gatccgtatg
     6481 gttaaccagc gccgtaatct actggactac ttaaagtcca aagacagcac acgatactcg
     6541 tcgctgatcc aaaaactggg tctacgccgc taagccaaag tgcccgccct aagcgggcac
     6601 ttttattttt agtccgatgt atttatcgaa tcgaaggata aaaaagtgaa tcctgtaata
     6661 aaacaatttc aatacggtaa agaaaccgta accctagaaa ctgggcgcat cgcccgccaa
     6721 gcgaccggtg ccgttgtcgt cagcatcgct caaacccgcg tattgtgtac cgtggtaggc
     6781 gccaaacaag ccagccccgg ccaaggattt ttccccctat ctgttcatta ccaagagaag
     6841 acctacgcag caggccgtat tcccggcgga ttttttaagc gtgaaggccg ccccagtgaa
     6901 aaagaaaccc ttacctcgcg cctgattgac cgccctatcc gcccgctttt ccccgaagga
     6961 tttttaaacg aagtacaggt ggtctgtact gtattgtctg ttgaaaaaga ccaagacccc
     7021 gatatcgccg ccatgattgg cacctctgca gccctcgcta tctccggcat tccctttaac
     7081 ggccccatcg gcgccgcccg cgtcggttac accgtaaaag acggttattt gctcaaccct
     7141 gccaacagcc aactgctcag ctccgagttg gacatggtgg tcgccggcac acaaaacgcc
     7201 gtgctgatgg tggaatctga agccaacgaa ctgcccgaag acatcatgtt gggcgctgtt
     7261 ctgttcgccc accaagagat gcaagcggtt attcaagccg ttaatgaact ggcccgcgat
     7321 gccgccaaac ccgc
//
LOCUS       DAOQGS010000161         7332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4944_length_7332_cov_4.628143, whole genome shotgun sequence.
ACCESSION   DAOQGS010000161 DAOQGS010000000
VERSION     DAOQGS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_7332_cov_4.628143"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="PKZ52_07790"
     CDS             <1..1890
                     /locus_tag="PKZ52_07790"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNG59714.1"
                     /translation="VEKQMIGVKSSVTMGALRSIRIFVLGEVMQPGSFTVGALSTMTN
                     ALFASGGINKVGSFRNIQLKRQGKVVTSLDLYDLLLRGDTSGDARLMPGDVLFVPPVG
                     ETITVMGAVTRPAIYELQNSADVAGALGLAGGASTNAYLPESRLERIGERGEKTLINL
                     DLTKARDKAQPVKDGDVLRISSVLDIVQDKVTLKGHIKRAEEFAWTRGARFSDAIPSV
                     DALLPNPDIDVALIQREIPGTRQIQTLLFSPKGAWANRGGAKDPLLQSNDTLYIFNFE
                     DDRSELLEEMIERLKTQAQFQERQKTVGISGSVRFPGVYPLAEQMSAQDLILLSGGLT
                     ESALGTQGEITRYDISQNMERVVMHVAVDLNDPRVQLQPADTLQIKQVPLWKNKESVT
                     IQGEVMFPGTYSILPGETLMDVLTRAGGLTPHAHSEGAVFSRKELRELEEARIQDMKK
                     TIESELAAASTTQQTGKKNIDTAEAQRILKSLEGSKAGGRLVIDLPLILKTPTGHDFQ
                     LEDGDTLTIPRFKPSVTVVGEVQFPTSHFYNAKLDASDYINNSGGTKKNADTARIYVV
                     KANGTVVSPKSGWLRASKGNIQPGDTIVVPLETDKKDTLEILAKASQIIYNSALGAAA
                     LAAIK"
     gene            1947..2023
                     /locus_tag="PKZ52_07795"
     tRNA            1947..2023
                     /locus_tag="PKZ52_07795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1981..1983,aa:Val,seq:gac)
     gene            2190..2265
                     /locus_tag="PKZ52_07800"
     tRNA            2190..2265
                     /locus_tag="PKZ52_07800"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2223..2225,aa:Asn,seq:gtt)
     gene            complement(2458..3039)
                     /locus_tag="PKZ52_07805"
     CDS             complement(2458..3039)
                     /locus_tag="PKZ52_07805"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNG59715.1"
                     /translation="MTDNLPEARGPWTCLTRQVAYENPWIRVQHETVTTPAGTPGIYG
                     LVHFKGRAVGVVPIDHEHHTYLVGQTRYTLNAYSWEIPEGGAHPNETLEACALRELEE
                     EVGLIASELVSLGTLHLSNSVTDESAHYYLATGLSPGQQKLDATEDIQVRRLPFKEAL
                     KMALDGQITDAISVAALLRLALERPEFLAPPKA"
     gene            complement(3083..4072)
                     /locus_tag="PKZ52_07810"
     CDS             complement(3083..4072)
                     /locus_tag="PKZ52_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59716.1"
                     /translation="MAAQIIAGVMVVLGALLGTRMGLGAVARQILATALALVLATYLA
                     PIVAAGLQAWLPYWLVWFSASLLLFFLVAVAAHRAMILFGQAMSLPRWLSRSGGALLM
                     GAVGALAAGLVLWTVQVAASLNPIAAQTPIGRPEGLYAWASKEVELLTRWSLRASGLS
                     EPTAASLGIVAAEPGFYGYQVVGIAISPELRALWQDPAAQPLLQQKAIQPLTQLPSAQ
                     QLLNLPMVAHLQQVLPVDAAQGQGLIMAQLANLWGLQQKLLQDPEIKALLADGTLVER
                     LAQQDLLGLFLNPYWRPLINRMQVLLSAEPSSRPTPDGLNGPIPAPESVIPAT"
     gene            4272..7148
                     /gene="gcvP"
                     /locus_tag="PKZ52_07815"
     CDS             4272..7148
                     /gene="gcvP"
                     /locus_tag="PKZ52_07815"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNG59717.1"
                     /translation="MATPAAHLASLSYSDEFIARHIGPSPSETQAMLAALGLASIEGL
                     MEKTLPAAIRSETTARLAGPCSENQALSELKAIAAGNRVFKSYLGLGYHPTFTPLVIL
                     RNVLENPGWYTAYTPYQPEIAQGRLEGLLNFQQMIMDLTGMEMANASMLDEGTAAAEA
                     MAMAKRVAKGNTSNRFFVHADVHPQTLAVVATRAEHFGFEIIQGSLDSLEAGDYFGAL
                     LQYPGSSGALVDLRAPIATIHNHQALAIVATDLLSLVMLEAPGAMGADIVVGSNQRFG
                     IPMGFGGPHAGFFAFRDEYKRSAPGRIIGVSIDKRGKQALRMAMQTREQHIRREKANS
                     NICTSQVLLALMSVFYAIYHGPEGLKRIAGRTHLLTKMLAAGLSQAGLRLRHSQFFDT
                     LVVETGPRTQALLEKAQAQGINLRNLQDALGISLNETTEEADLKDLLALFDAPCDIEK
                     LVQQAASQDALGTSLARSSSFLTHPVFNRYHSETEMLRYLKRLESKDIALNQAMIPLG
                     SCTMKLNATAEMIPVTWPEFANLHPFAPMEQAQGYATLFSQLQTMLQACTGYDAISLQ
                     PNAGSQGEYAGLVAIKKYFEAKGETQRRICLIPASAHGTNPASAQMVAMQVVVTACDD
                     AGNVDLADLRAKVELYGDQIAALMITYPSTHGVFEENIRDICQLIHSIGAQVYIDGAN
                     MNALVGLAAPGEFGGDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLASHPLQTI
                     EGTSASNGTISAAPWGSASILPISWMYIRMMGAEGVKQATQMAILNANYIAHRLKDHY
                     PILYTGRNGFVAHECLLDLRPLKAASGISEEDIAKRLMDYGFHAPTMSFPVAGTLMVE
                     PTESESKAELDRFCDAMLSIHREIQQVIDGTWPKDNNPLVNAPHSLDDVLDAAWDRPY
                     SRDCATRPLPYLHHHKVWPSVNRIDNVYGDRNLVCSCPSIEAYL"
BASE COUNT         1652 a         2239 c         1949 g         1492 t
ORIGIN      
        1 gtagaaaaac aaatgatcgg cgtgaaaagc tccgtcacca tgggggcttt gcgcagcatt
       61 cgcatctttg tgctggggga agtgatgcaa cctggctcct ttaccgtggg ggcactttcc
      121 accatgacca acgccctgtt cgccagtggc ggcatcaaca aagtcggttc atttcgcaac
      181 attcagctta agcgccaggg caaggtagtc accagcctcg acctctacga tctgctgctg
      241 cgcggcgata cctccgggga cgcccgccta atgccggggg atgtgttatt tgtgccgccg
      301 gtgggtgaaa ccatcacggt aatgggtgca gtaacgcggc cagctatcta tgaattgcaa
      361 aattccgccg atgtagccgg tgccctgggc cttgccggtg gcgctagcac caatgcctac
      421 ctgccggagt cgcgcctgga gcgcatcggc gagcgcggcg aaaaaaccct tatcaacctc
      481 gacctcacca aagcccgcga caaggcccag ccggttaaag atggcgatgt gctgagaatc
      541 tcctccgtgt tggatatagt gcaggataag gtcaccctaa aaggccatat caaacgggcc
      601 gaagaatttg cctggacgcg gggcgcccgc tttagcgacg ccatccccag cgtggacgcc
      661 ctgctaccca acccggacat agatgttgcc ttgatccagc gggaaattcc cggcactcgg
      721 cagattcaaa ccctcctatt ttcccccaag ggggcctggg ccaaccgagg cggcgctaag
      781 gatccgctgc tgcaaagcaa cgacaccctc tacatattta actttgagga tgatcgcagc
      841 gaactgcttg aggagatgat tgagcgctta aaaactcaag cccagttcca agagcggcaa
      901 aaaactgtcg gtatttccgg cagtgtgcgc ttccccggtg tgtacccact ggctgagcag
      961 atgagtgccc aagacctcat tttattatcc ggcggcctta ccgaaagcgc tttgggcacc
     1021 cagggggaaa ttacccgcta cgatatttcg caaaacatgg agcgggtagt aatgcatgtg
     1081 gcggtggatt taaacgatcc ccgcgtgcaa ctgcagcctg cggacaccct gcaaatcaaa
     1141 caggtgcccc tgtggaagaa caaagaatcc gtcaccatcc aaggcgaggt catgtttccc
     1201 ggtacctata gtattttgcc gggtgaaacc ctgatggatg tgctcacccg cgcgggcggc
     1261 ctcactcccc acgcccactc ggagggcgct gtattctccc gcaaggagtt aagggagctg
     1321 gaggaggcgc gtatccaaga tatgaaaaaa accattgagt ctgaattggc ggcggcctct
     1381 accactcagc aaaccggcaa aaagaatata gataccgccg aggcccagcg gattctcaag
     1441 agcctggaag gatcaaaagc tggcggccgc ttggtaatcg acttgccgct cattcttaaa
     1501 acccccaccg gccacgattt ccagctggaa gatggcgaca cactcaccat cccgcgcttt
     1561 aagccctcgg ttacggtggt gggcgaggtg cagttcccga cctcccactt ctataacgcc
     1621 aaactggacg cctccgatta catcaacaat tccggcggca ccaaaaagaa tgccgatacc
     1681 gcgcgcattt atgttgtgaa agccaacggc acagtggtgt cccccaaatc cggctggctg
     1741 cgggctagca aaggcaatat ccagccaggc gataccatag tggtaccact tgaaaccgat
     1801 aaaaaagaca ccttggaaat cctcgccaaa gccagccaga ttatttacaa ctcggccttg
     1861 ggtgcagcgg ctctggcggc gatcaaataa ttgagggata cggttgccca gggatagctt
     1921 agtcgttaga atgcgcgcct ctttgcagga ccgtagctca gttggttaga gcaccacctt
     1981 gacatggtgg gggtcagcgg ttcgagtccg ctcggtccta ccaattttta ccgccttaag
     2041 tgcgggctga ttttgtcttt taagcaagca aaatcaacca gttaagatgg aaaaatgggt
     2101 tgacagggga tggggcaatc gctaccatgc gcgccttcaa agaacgacgc ggtgttcaga
     2161 tcgcggtaaa ccgagcttca ccatttaatt ccgccttagc tcagtcggta gagcaaatga
     2221 ctgttaatca ttgggtcgct ggttcgagtc cagcaggcgg agccaaattc ttaaagcagc
     2281 gaagtgcacc aagctgcttt aaacactaaa aagcccttat tttaagggct tttttttcgc
     2341 cctaggtttt tacctcgttt acagcactgc cagatattgg ctaaaaccct ggcataagtg
     2401 gtggctcgac aaagcaaacc acccagcttt taaaggttat ccgcgcaggc ctcagtgcta
     2461 ggctttgggt ggcgcgagaa attccggccg ttccaaagcc aaacgcagca gggccgcaac
     2521 actgatggca tcggttatct gcccgtcgag agccatcttc aatgcttctt taaacggcag
     2581 gcgccgcact tgaatatcct cggtagcatc caatttttgc tggcccggcg agagaccggt
     2641 ggctagataa taatgggcgc tttcatcggt aactgagttg gaaaggtgca gcgttcccaa
     2701 gctgaccagc tcacttgcta tcaggcccac ctcttcctcc agctcccgca gggcacaggc
     2761 ctctagggtt tcgttggggt gggcgccgcc ctcgggaatc tcccaggaat aggcattgag
     2821 ggtgtaacgg gtttggccta ccaggtaggt gtggtgctca tggtcgatag gcaccacccc
     2881 caccgcccgc cccttaaaat gcaccaggcc atagatgccc ggggtgcccg cgggggtggt
     2941 aaccgtttcg tgttgtacgc gaatccaggg attttcatag gctacttggc gagtcaagca
     3001 ggtccagggg ccgcgggctt cggggaggtt gtcagtcatg gggcagtctc tagcaataaa
     3061 aaaatatcgg cgccaagtag gcttaggtgg ctggaatcac cgattcgggg gctggaattg
     3121 ggccgttcaa gccgtcaggt gtcgggcggg aacttggctc ggcacttaac aaaacctgca
     3181 ttctatttat taaggggcgc caataggggt ttagaaacag acccaagaga tcctgctggg
     3241 cgagccgctc caccaaagta ccgtcggcga gcagagcttt aatttcgggg tcctgcagca
     3301 gtttttgttg caacccccac agattggcca gctgcgccat gatcaaccct tgcccctggg
     3361 ctgcatccac cggcaaaacc tgctgcagat gcgccaccat cggcaggttg agtagctgct
     3421 gggcactggg taattgggtg agcggctgga ttgccttttg ttgcaacagg ggttgagctg
     3481 cagggtcctg ccacagagcg cgcagctcag ggcttatggc gatgcctacc acctggtagc
     3541 cataaaagcc aggctccgcc gcaactatac ccaggcttgc cgcagtgggt tcgcttaagc
     3601 cgctggcgcg caggctccaa cgggtcaaaa gctccacctc cttactcgcc caggcgtaga
     3661 ggccttcagg ccgtccaatc ggggtttgcg cagcaatagg gtttaggctc gctgcaacct
     3721 gaacggtcca aagcaccagg cctgccgcca aggcgcctac cgcccccatc aacaatgccc
     3781 cgccgctccg tgaaagccag cgcggcaaac tcatcgcctg gccaaataaa atcatcgccc
     3841 gatgggccgc cacggcgaca agaaaaaaca gcagcaagct ggcggagaac cacaccaacc
     3901 aataaggcag ccaggcctgc agacccgcgg ccacaatggg tgcaaggtac gttgccagca
     3961 ccagtgccag agcggtggcg aggatttgcc gggccacagc gcccaatccc atacgggtgc
     4021 ccaagagcgc acccaacacc accatcacac ctgcaataat ctgcgccgcc atttacttcc
     4081 tcgcattgat ttatggatat atacccctac tcgcccgctg aattggcgga cacaatgcga
     4141 ccgaacgctc agttgcattg ctgggctagg gtggccttgg taccatggcg cccttgtttg
     4201 caccggcccc acaggcgcgg gcacccgccg cttaactcta gaccacccgc ttttaatctg
     4261 aaggttcccc tatggccact cccgccgctc acctggcgtc tttaagctat tccgacgaat
     4321 ttatcgcccg ccacattggc ccaagcccca gcgaaacaca agccatgttg gccgccttag
     4381 gccttgcctc cattgagggc ctgatggaaa aaaccctgcc ggcagctatt cgcagtgaaa
     4441 ccaccgcccg cttagccggc ccctgctccg agaaccaagc cttaagcgag cttaaagcca
     4501 tagcggcagg caaccgagtg tttaaaagtt accttggcct gggttatcac cccaccttta
     4561 cacccctggt gatactgcgc aatgtgttgg aaaaccccgg ctggtacacc gcctataccc
     4621 cctatcaacc ggaaattgcc cagggccgct tggagggcct gttaaatttt caacaaatga
     4681 tcatggattt aactggcatg gagatggcca acgcctccat gcttgatgag ggcacagccg
     4741 ccgccgaggc catggctatg gccaaacggg tggccaaggg caatacctcc aaccgctttt
     4801 ttgtgcacgc cgatgtacac ccacagactc tggcagtggt ggcaacccgt gccgagcact
     4861 tcgggtttga gattattcaa ggcagcctcg atagcctcga ggctggggac tattttggcg
     4921 ccctcttgca ataccccggc tccagcggcg ccctggtgga cctgcgcgcg ccgattgcaa
     4981 ccattcacaa ccaccaggcc ctggccatag tagcgacgga cctgctaagc ctggtgatgc
     5041 tggaagcccc cggcgccatg ggcgcggata tagtcgtcgg cagcaaccag cgctttggta
     5101 tccccatggg ttttggcggc ccccacgcgg gcttttttgc ctttcgcgac gagtacaaac
     5161 gctccgcccc cggccgcatc attggggtgt ccatcgacaa gcgcggcaaa caggccctgc
     5221 gcatggccat gcaaacccgc gagcaacaca tccgccggga aaaggccaac tctaatatct
     5281 gtacctctca ggtgttgttg gctctgatga gtgtgttcta cgccatttac cacgggcctg
     5341 aaggccttaa gcgcatcgca ggccgcaccc atctgctcac caaaatgctc gccgcaggct
     5401 taagccaggc agggctgcgc ctgcgccaca gccagttctt cgataccctg gtggtggaaa
     5461 ccggcccgcg cacccaagcg ctgcttgaaa aagcccaggc ccagggcatt aacctgcgca
     5521 atctgcagga tgccctgggc attagcctga acgaaaccac ggaagaggcc gaccttaaag
     5581 acttgttagc cctgtttgac gccccctgtg atatcgagaa gcttgtacaa caggccgcaa
     5641 gccaagatgc cctgggcaca agcctcgccc gcagcagcag cttcctcacc catccggtgt
     5701 ttaaccgcta ccacagcgaa accgagatgc tgcgctactt aaagcgcttg gagtcaaaag
     5761 acatcgccct taaccaggcc atgatccccc taggctcctg caccatgaag ctcaacgcca
     5821 ccgccgagat gatcccggtg acctggccgg aatttgccaa cctgcacccc ttcgccccca
     5881 tggagcaggc tcagggctac gccactttat ttagccagct gcaaaccatg ctgcaggctt
     5941 gcaccggcta cgacgctatc agcctgcaac ccaacgcagg ctcccagggt gaatacgcag
     6001 gcctggtagc gattaaaaaa tacttcgagg ccaaaggaga aacccagcgg cgcatttgtt
     6061 tgatccctgc ctccgcccac ggcaccaacc ccgcgtcagc ccagatggtg gccatgcagg
     6121 tggtggtgac cgcctgtgat gacgctggca atgtggatct ggcggatctg cgggcgaagg
     6181 ttgaactcta tggcgatcag atcgccgccc tgatgatcac ctatccctcc acccacggtg
     6241 tgttcgaaga gaatatccgc gatatctgcc agctaattca ctccatcggc gcccaggtgt
     6301 atatcgacgg agccaatatg aacgccctgg tgggcctggc cgcccccggt gaattcggcg
     6361 gcgacgtatc ccacctgaat ctgcataaaa ccttttgtat tccccacggc ggcggcggcc
     6421 ccggcatggg ccccatcggc gtgaaagcac acctggcacc ttacctggcc agccacccat
     6481 tgcaaaccat cgaaggcacc agtgccagca acggcaccat ctccgccgcc ccctggggtt
     6541 cagcaagcat tctgccgatt agctggatgt acatccgcat gatgggcgcc gagggcgtta
     6601 agcaagccac ccaaatggcg atcctaaacg ctaactacat tgcccaccgg ctaaaagacc
     6661 actacccaat cctctacacc ggccgcaacg gctttgtggc ccacgaatgc ctgctggatc
     6721 tgcgccccct taaggccgcc agcggcatca gtgaagagga cattgccaag cgtttaatgg
     6781 actacggctt ccacgccccc accatgagct tccccgtggc aggcaccctt atggtggaac
     6841 ccaccgagag cgaatccaaa gccgagctgg atcgcttctg cgacgccatg ttgtctatcc
     6901 accgggaaat ccagcaggtt attgatggca cttggccaaa ggataacaac ccactggtga
     6961 acgcccccca tagcttggac gacgtattag atgctgcctg ggatcgccct tacagccgcg
     7021 actgcgccac ccgcccactg ccttatttgc accaccacaa ggtatggccg agtgtgaacc
     7081 gcattgataa cgtctatggg gatcgcaatc tggtgtgttc gtgcccgagt attgaggcct
     7141 atctctaaat ccctaggcct ggacggcaag caggtttgca actgctcgcc gcccaggcat
     7201 actccctcat cactgctctt ttttaaacaa gccttttaat tttccctcta actttttcac
     7261 ctgctcttta tccagagttg catctaactt ttcttttaat tccgcctttt tctccgcagc
     7321 tttttctttt at
//
LOCUS       DAOQGS010000162         7302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4972_length_7302_cov_4.286601, whole genome shotgun sequence.
ACCESSION   DAOQGS010000162 DAOQGS010000000
VERSION     DAOQGS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4972_length_7302_cov_4.286601"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKZ52_07820"
     CDS             <1..1065
                     /locus_tag="PKZ52_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNG59718.1"
                     /translation="EYSEPGSFTTKPDVAIVVFGENAYAEVQGDIQNLATLEVERKTK
                     VGLKTMQALKAQGIPVVAVLLSGRPLWVNKELNLADAFVAAWQPGTEGAGVADVLVAK
                     VDGTPNADFTGRLSFSWPATPCQTPLNKTDADYKPLFPFDYGLRYSSNLTPWQSLSES
                     TSDYTYGCRLKNTLPSAKRVEFKSKDGWEWRAELPSFAGDWVNQGITLGPLMVKPISA
                     GVTASWDGKGEARVLLRNKTTPRHDLLGALANQGALAVDVKLLAPVTEGPGGIDAKVW
                     TSPLTSGTVDISKLLATWPQGKTQTLSIDLACFAKTFADFAKVDGPFALQSKRALAVE
                     ISNLRYEPGIAKTASISCSN"
     gene            1405..3441
                     /locus_tag="PKZ52_07825"
     CDS             1405..3441
                     /locus_tag="PKZ52_07825"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNG59719.1"
                     /translation="MTDDDRTRLAPSEPRDDKTRIAQVNTAGVGAARPISEVPPSPAL
                     ASTPSPSHSSGDYTEYQVTGNDSSGFAKARALAQSTLASNGTLLKKRFVLEDVLGSGG
                     MGTVYKTRDLRKVEAEDPNPYIATKVLNQDFKHHPDAFVTLQQEAAKSHTLAHPNIVT
                     VHDFDRDGDILFMTMELLEGEPLDKLIKAHAGRGLGKERAFKLIQDMAAALGYAHQRN
                     LIHSDFKPGNVFVAADGRAKVLDFGIARAASVEAQKHSFDAGSLGALTPAYATLEMLR
                     GEAPSFSDDVYALACVAYEMLAGLHPYERRSALQALEKGLKPKRIETLSNRQWRALSH
                     ALALQQAQRTPTVAQFIAEFIPNRTATLVKIAAAAAALSLIGAGWFGYQQYQAKAKIK
                     TTIEEKLAEAQTCFAESRFACAVEQAMVVVSLDSKNTSAATLMKNAQLGLQKQQQATR
                     ISHLTSEAQNCLTEKNYACTQVKAREILDQEPKNTEAQALLAKADALIKSAEIAGLIS
                     QAEACLAKNDLGCAEVLNEQVSSINRQHPDAVALATKVIAAKNQSLQVVQATAQKAAS
                     LLSAGRTCLAQNNFSCAITQANAALAIDKTNPSAIELKQQAQAAQKQNAASQNTVDNL
                     LAEAQSCLDKKNYTCAIAKSEAALAVIPGNSQATSIRNRAVETQRKIKAGFSIQ"
     gene            3522..4208
                     /locus_tag="PKZ52_07830"
     CDS             3522..4208
                     /locus_tag="PKZ52_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59720.1"
                     /translation="MKKLSLALALASLTAVSAHAGLGLNDIKNSIDKSHKCDPSDQGC
                     KNKEHLKAAAKVAAVAVAAKIIADMVVEYQTQKVSDESKVIAEYKEKYKTLPPKPVAS
                     IYTTQTLPGKVVEPGKKVKIQSDIEVVPGSQQKEALIEERISIFDNEDNTKELKNMTK
                     QVNAQTKRGGRYKNEFSFQLPEGLPQGVYPIKTALLIDGQAAKTANNDIQLVLQVYSD
                     GQMQVLAQAF"
     gene            4828..4971
                     /locus_tag="PKZ52_07835"
     CDS             4828..4971
                     /locus_tag="PKZ52_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59721.1"
                     /translation="MPKISLSHCGALIVFCLSIISACDQPTPAVKNTQPPMLAVYTQL
                     IIP"
     gene            4993..5895
                     /locus_tag="PKZ52_07840"
                     /pseudo
     CDS             4993..5895
                     /locus_tag="PKZ52_07840"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
     gene            complement(5896..6369)
                     /locus_tag="PKZ52_07845"
     CDS             complement(5896..6369)
                     /locus_tag="PKZ52_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG59722.1"
                     /translation="MHKNTVCLWYEHNAEEAARFYTGLFPDSRMGAVHRAPGDYPDGK
                     AGDVLTVGFIVAGVPCMGLNGGPRFKHSEAFSFQIATETQEETDRYWHAIIDNGGEAS
                     YCGWCKDKWGISWQITPRVLVKTLAQGGDVAKRAFAAMMQMSKIDVAAIEAAVQG"
     gene            complement(6489..7181)
                     /locus_tag="PKZ52_07850"
     CDS             complement(6489..7181)
                     /locus_tag="PKZ52_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HNG59723.1"
                     /translation="MLRIFFLLYMIVFSLFVWGQTESECFGNPNNGSLKNGWQLPASG
                     ENFHAYSSIGALAGRNFVHSKVHRIVLEAYRILQETNPKIIYVYGETGFKSGGLFKPH
                     KSHRNGLSVDFFVPVVDSKGVPAELPIGISNKLGYNLEFDSLGRSGGLSIDFESIAKH
                     LDALFIAAKNQQSGIEVVIIDPRFRELLLATPAGAKINGKIRFSMQKPWVRHDEHYHV
                     NFEISCSVVDRN"
BASE COUNT         1955 a         2090 c         1696 g         1561 t
ORIGIN      
        1 gaatacagcg agccgggcag tttcactacc aagccggatg tagccattgt ggtgtttggc
       61 gaaaacgcct atgcggaggt tcagggcgat attcagaacc tcgccaccct ggaagtggag
      121 agaaaaacca aagtcgggct taaaaccatg caggcgctta aggcccaggg tattcccgtg
      181 gtggccgtgc tcttgagcgg ccgccccctc tgggtgaaca aagagctgaa cctggcggac
      241 gcctttgtgg ccgcctggca gccgggcacc gagggcgcag gggttgctga tgtgttggtg
      301 gcgaaagttg atggcacacc caatgcggat ttcaccggcc ggttgagttt cagctggcct
      361 gcaaccccct gccaaacgcc gttgaataaa accgacgccg actacaaacc cctcttcccc
      421 tttgactatg gcctgcgcta cagcagcaat ctaaccccct ggcaatccct gagcgaatcc
      481 accagtgact acacctatgg ctgccgcttg aagaacacat tgccgagtgc caaacgggtg
      541 gagtttaaga gcaaagacgg ctgggaatgg cgggcggaac tacccagctt cgcgggcgac
      601 tgggtcaatc agggcattac cctggggccc ttgatggtaa aacccatcag cgctggcgtg
      661 acggccagct gggatggcaa gggtgaagcg cgggttctac tgcgcaataa aactaccccc
      721 cgccacgacc tgttaggggc gcttgctaac caaggcgctc tagcggtgga tgtaaaactg
      781 ctcgcccctg tgaccgaagg ccccggtggt attgatgcca aggtgtggac cagcccccta
      841 acctccggca cggtggatat cagtaaactg ctggccacct ggccccaggg caaaacccaa
      901 accctcagca tcgacctggc ctgttttgcc aaaacctttg cggattttgc caaggtagac
      961 ggcccctttg ccctgcaatc taaacgcgct ttagcggtgg agatcagcaa tctgcgctat
     1021 gaaccgggta ttgctaaaac ggccagcatc agctgtagca actagtctca gcggtagcat
     1081 ttggccttag ctgtagcaat tggctaaggc acgcttcaca ccagggaatg ctgttcgttt
     1141 aggattttct cagccgtagg ggcgcccctc gtgggtgcca tgttccgttt attcgtcatg
     1201 ctcatgtgag ctttcagctg ggcaaccaca agggttgccc ctacagatca gccctcagta
     1261 atcatcgcca cgcggaagca ggtataagaa ttgttggctc cttatggctt tttgcctaca
     1321 gccgcgtgtg tttttgcgca cgcggaactt tcctttggcg tgctaaactc ccgactcatt
     1381 gaccgcttgg caaaggagtc cgtagtgact gatgatgacc gaacccggct tgcccccagc
     1441 gagccccgcg atgacaaaac ccgtattgcg caggtcaaca ccgccggggt aggtgccgcc
     1501 cgccccatca gcgaagttcc gcccagcccc gctcttgcta gcacacctag cccttcccac
     1561 agcagtggcg attacaccga ataccaagtc accggcaacg attcctccgg ttttgccaag
     1621 gcccgcgccc tggcccaaag caccctggcc agcaatggca cactgttaaa aaaacgcttc
     1681 gtgctggagg atgtgctcgg tagcggcggc atgggtacgg tgtataaaac ccgcgacttg
     1741 cgcaaagtag aggcggaaga ccccaatccc tatatcgcca ccaaggtgtt aaaccaggat
     1801 tttaagcatc acccggatgc ctttgtgacc ctccagcagg aggcggctaa atcccacacc
     1861 ctcgcccacc ccaacatagt cacggtgcac gacttcgacc gggatggcga catcctcttt
     1921 atgaccatgg agctgctgga aggcgagccc ctggataagc ttatcaaggc ccatgcgggc
     1981 cggggcctgg gtaaggagcg ggcatttaaa ctgattcagg acatggccgc cgcccttggc
     2041 tacgcccacc agcgcaatct tattcactcg gattttaaac cgggcaacgt atttgtggcc
     2101 gccgatggcc gcgccaaggt attggacttt ggtatcgccc gggccgcctc ggtggaagcg
     2161 caaaaacaca gcttcgatgc cggcagcctc ggcgccctca ccccggctta tgccacttta
     2221 gaaatgctgc gcggcgaagc gccgagtttt agcgatgatg tgtatgccct ggcctgcgtg
     2281 gcctatgaaa tgctggcggg ccttcacccc tatgagcgcc gctccgccct gcaggccctt
     2341 gaaaaaggcc ttaaacctaa gcgcattgaa accctgagca accgccagtg gcgcgccctc
     2401 agccacgccc tggccctgca acaggcccag cgcaccccga cggtggcgca gtttatcgcc
     2461 gaatttatcc ccaaccgcac cgccacactg gtcaaaatcg cagcggcggc agcggcgctg
     2521 tcgttaatcg gcgccggttg gtttggctac cagcagtacc aggccaaggc caaaataaaa
     2581 accaccattg aagagaagct ggccgaggcg caaacctgtt ttgccgagag ccgctttgcc
     2641 tgcgccgtgg agcaggccat ggtggtggtg agcctggaca gcaaaaacac cagcgccgct
     2701 acgctgatga aaaacgccca gctgggcctg cagaaacaac aacaggccac ccgcatcagc
     2761 catttaacga gcgaagcaca aaattgttta acggagaaaa actacgcctg tactcaggtt
     2821 aaagcgcggg aaattctcga ccaagagccc aaaaatactg aggcccaggc cctcttagcc
     2881 aaagccgatg ccctaattaa aagcgcggaa attgcaggcc tcatcagcca ggctgaagcc
     2941 tgtttagcca agaatgattt aggctgcgcc gaggtgctta acgaacaggt cagctccatc
     3001 aaccggcagc acccggatgc cgtggccctg gccaccaagg ttatcgccgc gaaaaaccag
     3061 tctctgcaag tggtgcaagc caccgcgcaa aaagccgcca gcttgctcag tgctgggcgg
     3121 acctgtttgg cgcaaaataa tttttcctgc gctattaccc aagccaatgc ggccctggcg
     3181 attgataaaa ccaacccctc ggccatcgaa ttaaaacaac aggcccaggc ggcgcaaaaa
     3241 cagaatgcgg cctcacaaaa tacggttgat aacttactgg ccgaagcgca aagttgcctg
     3301 gataaaaaaa attacacctg cgccattgcc aaaagtgagg cagctttagc ggttattccc
     3361 ggcaattcac aagccaccag cattcgcaac cgcgcggtcg aaactcagcg caaaataaaa
     3421 gccggatttt ccattcaata aaaatttttt aaaccaactt atttttaaac caacttattt
     3481 ttaaaccaac ttatttttaa accaactaaa ggaaccactc tatgaaaaag ctcagcctcg
     3541 ctcttgccct cgcctccctc accgccgtct ccgcccacgc agggctcggc ctaaacgata
     3601 ttaaaaactc catcgacaaa agccacaaat gcgatccatc cgaccagggc tgcaaaaaca
     3661 aagaacattt aaaagctgcg gccaaagtgg ctgcggtggc ggtagcggca aaaatcattg
     3721 ccgatatggt ggtggaatac caaactcaga aagtgtccga tgaaagtaaa gtcatcgccg
     3781 aatacaaaga aaaatacaaa accctgccac ccaaaccggt tgcctctatt tacaccacac
     3841 aaaccttgcc cggcaaagtg gtggagccgg gtaaaaaagt aaaaattcag tcggatattg
     3901 aagtagtgcc cggctcccaa caaaaagaag ccctaattga agagcgcatc agcatctttg
     3961 ataacgagga caacaccaaa gaattaaaaa acatgaccaa acaggtcaac gcgcaaacca
     4021 aacgcggcgg ccggtataaa aatgaatttt cctttcaatt acccgaaggc ttgccgcagg
     4081 gcgtataccc catcaaaacc gctctgctca ttgacggcca ggcggcaaaa accgcaaata
     4141 acgatattca gctagtattg caggtgtatt cagacggcca aatgcaggta ttagcgcagg
     4201 ccttttaata ttgcggcgct tatacccggc accgtttaaa agcgcctgca ggcgcttttt
     4261 tatgggttaa atagacacct tttaaaggcc cctgtttagt ttaagccccg cgtgattcac
     4321 ctattaaccc ctggcgccct cacggactta gaaacgcttg gccatgctta tatttatcgt
     4381 aactatttcg gcacacctta ttaccccctt ccgcacgggc gacacattca ggcggattta
     4441 gccaagcgga agaatcaatt cgcggtgcag ctgggatgaa ttcaggttat tttagaccgt
     4501 aaaaagaagg acctttttga cgagcataca cggatatatt cacggcgtgt ctacaataac
     4561 ctgtattcat aactgccgag gagtaactta gggctcacaa cactcacgca ttcgcctgct
     4621 gatttctatt cggcgcgaga atcatctcca gctaccacaa agtggcgctg aatagttacc
     4681 caaatagtta ctattttatt taatcagcca gttgccataa caggctgccg ccgcaggcaa
     4741 ttagggaacc tctgcggcat aatcccatcc gtttatcgct tcggatatac acacccattg
     4801 aacatcggct tatcggtaac caacctaatg cccaagattt ccctgtcgca ctgcggcgct
     4861 cttattgtgt tttgcctcag catcataagc gcctgcgatc aacccacccc agcggtaaaa
     4921 aatacccagc cgcccatgct cgccgtgtac acgcagctaa ttattcccta gcaccaaggg
     4981 tttgcccaag ccaataaaac actggtagaa cggattgagc gtttctgcca aaaccccgcc
     5041 aacccaggtg aataccaaga agcgcgcagc gcctggcagg cgagttttat cgcctggcaa
     5101 ccgctcaatc ttttacattt cggcccctta tctctaggcg atctgcggct gcatatatat
     5161 ttttggccgg atcgttttaa ttgggtggaa aaacacctga aaaaccggct aaaccaaagc
     5221 gacactgcta ctgacaccac aggcccggcc agtgctttgg gccttgccga catggaatac
     5281 ctattatttg ataccgccta caccgacaac gccgccctag ccggtgccac ctgcgatcta
     5341 ctgcgcagtg ccagcagcca aattaataac cgcgcgcagg aattattaaa ccactggcaa
     5401 ctgggcacgc tgggcgcctg gcaggcccca tcattagcct cacgctcaga cgcacaaaca
     5461 ctggccctta agaatcttat cgatagttta atttccagtt tcacacggat aacccaagag
     5521 gaattaattg cccccatcgg cagcctcgac acccccgcag caaatccctt aatcagccaa
     5581 gcctggcgtg caaacctagg caaggcggca ctattaagcg aacttaaagc ggccaacgca
     5641 ctgctgcatc tgggcctggc accccttgca aaaaccccgc agcagcaggc attacaaagg
     5701 acattaaaag agcatttaaa tcaattgcac gcggaattaa ccaacacccc aggtgaactg
     5761 gtttttttaa ttaacaccga attaggccgc aaacaattga ttcacgcggc tgaagaaatg
     5821 caaaaaatga cggcgatgtt aactaggctc gcggacagcc tagcagcgca agagcaccca
     5881 caacccactc attaattagc cctgcaccgc cgcctcaatt gccgccacat caattttact
     5941 catctgcatc atggcggcaa aagcgcgttt agccacatca ccgccctggg cgagagtctt
     6001 cactaaaaca cgcggtgtga tctgccagct aataccccat ttatctttgc accaaccgca
     6061 gtaactggcc tccccgccgt tatcgataat ggcgtgccaa taacgatcgg tttcctcttg
     6121 agtttcagta gcaatttgaa atgaaaatgc ttcgctgtgt ttaaaacgcg ggccgccatt
     6181 tagccccata cacggaacac ccgccacaat aaaacccacc gttaaaacat ctccggcttt
     6241 gccatctggg taatctcctg gcgcacgatg cacagcgccc atgcgactat cgggaaacag
     6301 ccccgtataa aaacgcgcgg cttcttctgc gttgtgttcg taccataggc aaactgtgtt
     6361 tttatgcatt ttccaattct caaggtcaac caataattct actatctaaa acgcatttac
     6421 gatttacaat cgaataagat ccatgaactg cactgccaaa ataaattctt tgtaactaca
     6481 agacattttt agttgcgatc aaccacagaa caggatatct caaaattgac gtgataatgt
     6541 tcgtcgtgcc ttacccaagg tttttgcata gagaatctga ttttaccatt aattttagcc
     6601 cccgcaggag ttgccagaag aagctcccga aaacgcggat cgatgataac gacctcaata
     6661 ccactttgtt gattttttgc agcaataaag agtgcatcaa ggtgttttgc gatggattca
     6721 aaatcgatag acaatccacc cgatcgccca agggaatcaa attccagatt gtaaccaagt
     6781 ttattactga tgccgatagg aagctcagca ggcacccctt tggaatcaac cacaggcaca
     6841 aagaaatcaa ctgataagcc gttgcgatgg cttttatgtg gcttgaataa tccgccactt
     6901 ttaaagcctg tttcgccata gacgtaaatt attttcgggt tggtttcctg caaaattcta
     6961 taagcctcca acacaatccg atggacttta ctatggacaa aattgcgtcc cgctagcgct
     7021 ccaatcgaac tgtaagcgtg aaaattttcg ccactggctg gcagctgcca accatttttc
     7081 aagcttccat tattaggatt accgaagcac tcactttccg tctgacccca aacaaaaagc
     7141 gaaaacacga tcatataaag cagaaaaaat atccttaaca taattctgtc caaatagttt
     7201 gattaggaac cgttggagat attcaacatc acgcaacaat aaatgaattt gtcacttaca
     7261 tccataaata actgtctaat gaatccccgg ataagagcaa ac
//
LOCUS       DAOQGS010000163         7289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_4978_length_7289_cov_5.187725, whole genome shotgun sequence.
ACCESSION   DAOQGS010000163 DAOQGS010000000
VERSION     DAOQGS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4978_length_7289_cov_5.187725"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="PKZ52_07855"
     CDS             <1..1637
                     /locus_tag="PKZ52_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HNG59724.1"
                     /translation="LLMDNDASDNVQLPALYTQMAPQQRRQRAGELLSQLGLAERLHH
                     RPGQLSGGQQQRVSIARALMNGGQIILADEPTGALDSHSGEEGMALLGELSRRGHTII
                     LITHDAKVAAFAQRQIELKDGQIISDTGAAPGAAAPLTGRAKGDAPRLRELATALHSA
                     LRCLGNNLFRTALTLLGIVIGVASVITLMAIGDGAKNAVVENISAMGSNLLLVRPGGP
                     NQRPRWEASNLVPEDVDAIAALPGIAAALPEQSSSVTLRIGNSDHTTEALATGANYPL
                     ARKWAPSQGTFFSQEDDDQYATVVVLGQTVKQKLFGEQNPLGEYIMIKNLLFQVIGVM
                     APKGADPGGQDQDDKVLVPFKTGSLRLNGQRTLRNITISVAEPDQMDSTQARVRELLL
                     ERHGTEDFQIRSMSSLIDTVSATQNTMTLLLGAIAAISLLVGGIGVMNIMLVSVTERT
                     REIGIRMATGARQRHILQQFLLEALVVSSLGGAIGVALGLGIALLAGQLGTSVTFSLW
                     PVLLAFSCAFATGLVFGFLPARKASGLNPVVALAAQ"
     gene            1655..3100
                     /locus_tag="PKZ52_07860"
     CDS             1655..3100
                     /locus_tag="PKZ52_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNG59725.1"
                     /translation="MPRNLRSNCLLTAWLFSLLMLCACSQQAAKATAPALGALASAPA
                     PALSSQAQAIAASQVPFTASGSEPSRLANWQAFGSPGLERMLAEAAAHSPEGAAARAR
                     LEQAQARYGLSRASLWPQLGLSVSSGKSRSKLPQGEVIQQGNSRASLSLEQDLDLFGA
                     ERASRRSASYSLKASTLRAGADQLELNLAIAQAWFTHLALNERYSTQSQNLAIAQQIL
                     QLVEARFKAGAVSAADLSQQRTNLLAQQAALLPIEAQRQANLAALAVLVGAAPQTFNP
                     PAEALSQLRVPALAAQWPAQVLTQRPDIGILEAQLAAADADISQARAAFFPRLSLGAA
                     ASLTSGELFSLNPATQGLSWSLNLAQSLFDGGAKRNQYRLAKAQRLELVETYRKAVLN
                     ALAQSQTALANAGINQQQEEQQRQLVEEAQRSLALTQARYKAGRGDLQNLLDAQRSLF
                     SAQDGLQQKCQARLLGALDLVQALGGWPQGS"
     gene            3492..4022
                     /locus_tag="PKZ52_07865"
     CDS             3492..4022
                     /locus_tag="PKZ52_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNG59726.1"
                     /translation="MLAVTGISHPACAEGNYVGIGFSPYSLEAEANGSSVSGDANVLE
                     GYYGFEWNDFFAAELRLGFGLGSGDLELDSNIYSGDVGKVKITSMYGLYAKPQFKSGG
                     FKAYALLGYAGYSTETTIEYGSYSESEDTTDDGFSYGIGAGFDFGQHGIALEWKTTKI
                     DGGDISGVAAFYQYQF"
     gene            complement(4190..4525)
                     /locus_tag="PKZ52_07870"
     CDS             complement(4190..4525)
                     /locus_tag="PKZ52_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59727.1"
                     /translation="MSHKTSAKSPMILTLRYPHVRELVFHYATKLTDTAVLKILDTGV
                     NSAADAEHLSYFIWRMIGEMAKDRAAGIEVLGGTDNTSMGPDIAYEMDNLMQVSGFSD
                     IWETISDEY"
     gene            complement(4515..5204)
                     /locus_tag="PKZ52_07875"
     CDS             complement(4515..5204)
                     /locus_tag="PKZ52_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNG59728.1"
                     /translation="MDKQVEVLAIVEGKTEQIFIEAILAPYFAEKGIYLRATQVSKPG
                     QKGGDVKFSRTIKDIGNHLKQRSDTYVATFVDYYGIKEWPGLDKVPPQASPTKIAEVV
                     NLETCLLVATTYPELQTHTRYIPYLAVHEFEALLFSCSETLAAKLSIKLEQVEKVLSD
                     CGEPEAINNSPITAPSKRLDAWVIGGKFKKTSAGISIASSIGISKMREKCPVFNTWLL
                     ALERLVAVNEP"
     gene            complement(5197..6300)
                     /locus_tag="PKZ52_07880"
     CDS             complement(5197..6300)
                     /locus_tag="PKZ52_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG59729.1"
                     /translation="MSAIDKITIKGFKSIKALEEFDLKSLNIIVGANGAGKSNLISFF
                     KLLRDLIDGKLNRYVRDNGGATDLLFNGRKVTEKMVFETRFGNHGFRFSLVPTPKDDC
                     AIEDEARYYSGGKTGWWNLGDSDDGKSKMVAEVTTNKPDGRYSKPVYDAIRSWQIYHF
                     HDTSATAAMRGYEIVQDNTFLRADAANIGPFLLALKQDHNNAYRQIIDAIKLVMPFFA
                     DFILQPRKSGEKEEVNISWNQEGSDYPMQPYQLSDGSIRFICLATALLQPKPPSTIII
                     DEPELGLHPAAIVILAELIQSASTRTQVIVATQSPALIDQFAIDDIVVVNRREGASSF
                     ERLQEKDYSQWLEDYSVGELWTKNVIAGGPTYG"
     gene            complement(6618..6803)
                     /locus_tag="PKZ52_07885"
     CDS             complement(6618..6803)
                     /locus_tag="PKZ52_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59730.1"
                     /translation="MPNATPIRVYNANTLRRLEEAKPTSDISSSTMSEVAPLFQPTKI
                     KSEREATATNKKAKKNA"
     gene            complement(6796..7113)
                     /locus_tag="PKZ52_07890"
     CDS             complement(6796..7113)
                     /locus_tag="PKZ52_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59731.1"
                     /translation="MNKPRYAFADTMQNTSLCFALRASLRLPKFDPVEFLERKANAAL
                     ERVNNLTQSILAKAFKGELTAHWRAANSDLISGENSAEALLKKIRSEREAKATNKKAK
                     KNA"
     gene            complement(7110..>7289)
                     /locus_tag="PKZ52_07895"
     CDS             complement(7110..>7289)
                     /locus_tag="PKZ52_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59732.1"
                     /translation="NNLTQSILAKAFKGELTAHWRAANPDLICGENSAEALLKKIKSE
                     REAQIPTGKKRKAGA"
BASE COUNT         1581 a         2011 c         1956 g         1741 t
ORIGIN      
        1 atctgctgat ggataacgat gccagcgaca atgtgcagtt gcccgcgctg tacacccaaa
       61 tggcgccgca gcagcgcagg cagcgggcgg gggagttgct cagccaattg ggcctggccg
      121 agcgattgca ccatcgcccc ggccagctct ccggcggcca gcagcagcgg gtgtccatcg
      181 cccgggcctt gatgaacggc gggcagatta tcctcgccga tgaacccacc ggcgccctgg
      241 acagccacag cggcgaggag ggaatggcgc tgttagggga actctcccgc cggggccaca
      301 ccattatttt gattacccac gacgccaagg tggccgcctt cgcccagcgc caaattgagc
      361 tgaaagacgg ccagattatc agcgatacgg gggccgcgcc gggtgctgct gcgcccctga
      421 cgggccgggc caaaggggat gcgccgaggc tgcgtgagct ggccaccgcc ttgcacagcg
      481 ccctgcgctg cctgggcaat aatctgttcc gcacggcgct tacgctgctg ggcatagtga
      541 ttggggtggc ctcggtgatc accctgatgg ccataggcga tggcgctaag aatgcagtgg
      601 tggagaacat cagcgctatg ggcagcaacc tgctgttggt gcgccccggc ggccccaacc
      661 agcggccccg ctgggaagcc agcaacctag tgcccgaaga cgtagatgcc atagccgccc
      721 tgccgggtat agccgccgcc ctgccggagc agagcagcag cgtcaccctc cgtattggca
      781 atagcgatca caccaccgag gccctggcca ccggcgccaa ctacccctta gcgcgcaaat
      841 gggcgcctag ccaaggcacc tttttcagcc aggaggacga cgaccaatac gccaccgtgg
      901 tggtgttggg gcagacggtg aaacaaaaac tcttcggcga gcaaaacccc ctgggcgaat
      961 acatcatgat caaaaacctg ctgttccagg tgatcggcgt catggccccc aagggcgccg
     1021 accccggcgg ccaggatcag gatgataagg tactggtgcc gttcaaaacc ggcagcctgc
     1081 gcctgaacgg ccagcgcacc ctgcgcaata tcaccatctc cgtggcggag ccagaccaaa
     1141 tggacagcac ccaggcgcgg gtgcgggagc tgctgctgga gcgccacggc acggaggatt
     1201 ttcaaatccg ctccatgtca tccctgattg acactgtctc cgccacccaa aacaccatga
     1261 ccctgttgct gggggcgatt gcggcaattt ccctgttggt ggggggcata ggggtgatga
     1321 acattatgct ggtgagcgtt accgagcgca cccgcgagat cggcatccgc atggccaccg
     1381 gcgcccgcca gcgccacata ttgcagcagt ttttgctgga agccctggtt gtgtcgtccc
     1441 taggcggcgc tataggcgtg gccctagggt tggggattgc cctcttggcc gggcagctgg
     1501 gcactagtgt gaccttcagc ctgtggcctg tacttttggc cttctcctgc gcctttgcca
     1561 ccggcctggt gtttggcttc ttacccgccc gcaaagcctc cggtctcaac ccggtggtgg
     1621 ccttagccgc ccagtaagtt ttttggtgat agttatgccc cgcaaccttc gttctaattg
     1681 tttgctcacc gcctggctgt tcagcctatt gatgctctgc gcctgtagcc agcaagccgc
     1741 caaagctact gctccagcgc ttggcgctct ggcttcagct ccagccccgg ccctaagctc
     1801 ccaagcccag gcaattgccg caagccaggt gccgtttacc gcaagcggca gcgagcccag
     1861 ccgcttggcc aactggcagg cctttggctc ccccggcctt gagcggatgt tagccgaggc
     1921 cgccgcccac agccccgagg gcgccgccgc ccgcgctcgc ttagagcagg cccaagcccg
     1981 ctacggcctc tcccgcgcca gcctctggcc ccagctgggc ttaagcgtca gcagcggcaa
     2041 aagccgcagc aaactgcccc agggggaggt aatccagcag ggcaacagcc gggcgagcct
     2101 gagcctggag caggatttag acctgttcgg cgccgagcgg gccagccgcc gctcggccag
     2161 ctacagcctt aaagctagca ccctgcgcgc gggggccgat caactggagc tgaacctcgc
     2221 catagcccag gcctggttta cccatctggc cctcaacgag cgctacagca cccaaagcca
     2281 aaacctcgct attgcccagc agattctgca gctggtggag gcccgcttta aggccggggc
     2341 ggtgagcgcc gccgacctct cccagcagcg cacaaatttg ctcgcccagc aggcggccct
     2401 tttgcccatt gaagcccagc gccaggctaa ccttgcagct cttgcagtat tggtaggcgc
     2461 agcgccgcaa acctttaacc ccccggcgga agccttaagc caacttaggg tgcccgcgct
     2521 tgccgcccag tggcccgctc aggtgctcac gcagaggcct gacataggca tactggaggc
     2581 gcaattggcg gccgcagacg ccgacattag tcaagcccgc gccgccttct tcccccgcct
     2641 aagcctaggg gcagctgcaa gccttaccag cggcgagctg ttcagcctca accccgccac
     2701 ccaaggtctc agttggagtt tgaacctcgc ccaaagcctg ttcgatggcg gcgccaagcg
     2761 caaccaatac cgcttggcta aggcccagcg gctggagctg gtggaaacct accgcaaagc
     2821 ggtgctcaac gccctggccc aaagccaaac cgcccttgct aacgcaggta ttaatcagca
     2881 gcaggaggaa caacagcgcc aactggtgga ggaagcccag cgcagtctgg ccctcaccca
     2941 agctcgctat aaagccgggc gcggggacct gcaaaacctg ctggacgctc agcgcagcct
     3001 gtttagcgcc caagacggcc tgcagcaaaa atgccaggct cggttattgg gggcattgga
     3061 tttggtacag gcattggggg gttggccgca gggttcttaa cagttgagtg caaacagggt
     3121 taatttgcgg cgccacgccc actgccgtta accagtaagt tgccccccct aattaaggaa
     3181 ataagcaaag agttgtcggc ggtttctcgc catgggcggc aatgtcatta agttaaggaa
     3241 tattcctaac ccataagtac agtaggggcg ccccttgtgg gtgcccatac ctactaaaac
     3301 ctaaagggtt atctggcgct caccgggcta ccaaacgggt tgcctctacg gatttgcctt
     3361 aacttaagcg ccccgcaagt gatatgcaca ttccttgctt cgtcacacga gctgattaaa
     3421 atggcggccc ccatgtcggg gtgttttaac aggagtctta ctatgacaaa acaacttccg
     3481 cattggccct tgtgttggcc gtaacaggaa tttcccaccc agcttgcgct gaaggcaact
     3541 acgtaggcat aggctttagc ccctattctc tcgaagcaga ggcgaatggc tcaagcgttt
     3601 ccggcgacgc caatgtgtta gagggttact acggctttga gtggaatgat ttcttcgcgg
     3661 ccgaattgcg cttagggttt ggccttggca gcggcgatct tgaattagac agcaacatct
     3721 acagcggcga cgtgggcaag gtaaaaatta cttccatgta cggcctgtac gccaaaccgc
     3781 aatttaaatc cggcggcttt aaagcctatg ccctcttggg ttatgccggt tacagcactg
     3841 aaaccactat cgaatacggc tcctacagcg aatctgaaga caccaccgac gatggtttta
     3901 gttacgggat aggtgcaggc tttgattttg gccagcatgg cattgcccta gagtggaaaa
     3961 ccaccaaaat tgacggcggc gatatcagtg gcgtagcggc gttttaccaa tatcagtttt
     4021 aataaaaact gtttttaaaa aaggggtgca atcccctttt ttatctgcaa aaggcattac
     4081 tgccaggcgg atatttctcc ttatcgcacc aaacctaaaa actatagagg cctattttta
     4141 ctcgagtgaa tgcaacttca cacctcatta aagattaagt gccgattctt caatactcgt
     4201 cagatattgt ctcccaaata tcactaaacc cactcacctg cataaggtta tccatttcat
     4261 aggcgatatc cggccccatg gaggtattgt cggtgccgcc gagcacctca attccggctg
     4321 ctcggtcctt ggccatttcc ccaatcatgc gccaaataaa ataactgagg tgttcggcat
     4381 cggcggctga gttaacgcct gtatcgagta tttttaatac ggcagtatcg gtaagttttg
     4441 tagcgtagtg aaaaaccaat tctcgtacgt gggggtagcg tagggtgagg atcatagggc
     4501 ttttggcgga tgttttatgg ctcattaacc gccaccagcc gctctaaggc caagagccaa
     4561 gtattaaaaa cagggcattt ttctcgcatt ttactaatgc caatactact ggcaatcgaa
     4621 atacctgctg aggttttttt gaatttacca ccaataaccc aagcatcaag acgcttagac
     4681 ggtgctgtta tcgggctgtt attaatagct tcaggctcgc cgcaatcact taatactttt
     4741 tcaacctgct ccaacttgat gctaagttta gcggcgaggg tttcactaca gctgaataat
     4801 agcgcttcaa actcatgtac cgccaaatag ggtatgtagc gggtatgggt ttgtaattcg
     4861 gggtaggttg tagccactaa aaggcacgtt tccaaattta caacttccgc aattttagtg
     4921 gggctcgctt gaggcggcac cttgtccaag ccgggccact ctttaatccc gtagtaatcc
     4981 acaaatgtgg ccacataagt atccgacctt tgtttaaggt ggttgccgat atctttaatg
     5041 gtgcggctaa atttcacatc accgcctttc tggccgggtt ttgacacctg agtggctcta
     5101 aggtaaatgc ctttttccgc aaagtaaggt gcaagaatag cctctataaa aatttgctcg
     5161 gtttttccct caactattgc cagtacttcg acttgtttat ccataggtgg ggccccctgc
     5221 gataacgttt ttagtccata attcacccac tgagtaatcc tctaaccact ggctatagtc
     5281 cttttcctgt aaacgctcaa aactagaagc gccttctctg cggtttacaa cgactatatc
     5341 gtcaatagca aattgatcaa ttagtgctgg cgattgcgtg gccacaataa cctgggttcg
     5401 tgtggaggca gactgaatca gttcagccag aataacaatc gccgccggat gtaaacccag
     5461 ttccggctca tcaataatta ttgtgctggg tggtttaggc tggagtagcg ccgtggccaa
     5521 acagataaat ctaatagagc catcggataa ttgatacggc tgcattgggt aatcagagcc
     5581 ttcctggttc cagctgatat tcacttcttc tttttcgcca cttttgcgcg gttgcaaaat
     5641 aaagtctgcg aaaaatggca tcaccaattt aatcgcatca attatttgcc ggtaagcgtt
     5701 attatggtcc tgcttcagtg caagtaaaaa tgggccaata ttggcggcat ctgcgcgtag
     5761 aaaggtattg tcttggacta tttcatagcc gcgcatggcg gcggtagcgc tagtgtcgtg
     5821 aaaatggtaa atctgccagg aacgaatagc atcataaact ggcttggaat atcgaccatc
     5881 gggtttgttg gttgttactt ccgctaccat ttttgacttg ccgtcgtcgc tatcgcctag
     5941 gttccaccac ccggttttac cgccactgta ataacgggcc tcgtcttcaa tagcgcaatc
     6001 atcctttggg gtgggtacca agctaaaacg gaacccgtgg ttgccgaagc gtgtttcaaa
     6061 aaccattttt tccgttactt tgcggccatt gaataataag tctgtggcgc caccgttatc
     6121 gcgcacatag cgatttaact tgccgtcaat taaatcccgc agcagtttaa aaaatgaaat
     6181 caaattactt tttcccgctc cattagcgcc gacgattatg ttaaggcttt tcaggtcaaa
     6241 ttcttcaagt gcctttatcg acttaaagcc cttaattgtg atcttatcta ttgcgctcat
     6301 gggtgtacct ttaggcttgg gttttggcgt ttgactgact tggttcttgt tcggatttta
     6361 ttctggaaga ttggaagagc gtatggtcac atggatgtga cacagtagag caatgcagga
     6421 gcaattgccg agcgacttcc gatattgtgc ttgatgagat gtcggaagtc ggcttcgcct
     6481 cttccaacct acgcagggta tggcaatttt gtagggtggg caatttattg cccaccagat
     6541 tattgccgca aaaggtgggc aacaagttgc ccaccctacg aaggaaattg gggctgtaca
     6601 tacgcgtcgg cagggcgtta ggcatttttc ttcgcctttt tatttgtggc cgttgcttcg
     6661 cgttctgatt ttatttttgt aggttggaag agcggagcga cttccgacat tgtacttgag
     6721 gaaatgtcgg aagtcggctt cgcctcttcc agcctacgca gggtattggc gttgtacact
     6781 cgaatcggcg tggcgttagg catttttctt cgccttttta tttgtggctt ttgcttcgcg
     6841 ttcggatctt atttttttaa gcaatgcctc ggcgctgttt tcgccgctga ttaaatccga
     6901 gttggcggcg cgccagtggg cggtgagttc gcctttaaag gctttggcta gtatagattg
     6961 ggttaggttg ttgactcgct ctaatgctgc gttggctttt cgttcgagaa attcgacggg
     7021 atcgaatttc ggcagccgaa ggctggcccg cagggcaaaa cacaaggatg tgttttgcat
     7081 tgtgtcggcg aaggcgtatc tcggtttgtt catgccccag cctttctctt tttacccgtt
     7141 ggaatttgcg cctcgcgttc tgattttatt tttttgagta aagcctcggc gctattttcg
     7201 ccgcagatta aatccgggtt ggcggcgcgc cagtgggcgg tgagttcgcc tttaaaggct
     7261 ttggctaaaa tggattgggt taggttgtt
//
LOCUS       DAOQGS010000164         7248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5019_length_7248_cov_4.482552, whole genome shotgun sequence.
ACCESSION   DAOQGS010000164 DAOQGS010000000
VERSION     DAOQGS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_7248_cov_4.482552"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PKZ52_07900"
     CDS             <1..1690
                     /locus_tag="PKZ52_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG59733.1"
                     /translation="MPVLIALADDKGNLLMASAQHGELEGVAGNCEHWLKLADVYPAS
                     FQNDLESLLAAQSTSPEWLEFKAKHKDGSFHVYQIKYIYHQSAGQSLCFVFGTDITAH
                     KQIENSLRDHKSRLDYMVYHDNLTGLANRTLFYGCINKTLEFARENHETLGILLVDLD
                     RFKNVNENLGYDAGDDLLRITADNLRAQLREKDVVARLSGDEFVVVLDDVRRKSDLEL
                     VAQKLLNRLSLPVKLQGHSIISTASIGISVFPADGDNIDQLLKHADLAMHKAKAKGKN
                     CFEFYEEALHNQAINYLLLENDLRRAIDQGEIILHYQPQINLTNGRIVGLEALVRWNS
                     KERGMVPPMHFIPLAEETGLIEPLGEWVLRHACERFQAWLMKGLKLGKMAVNISPVQF
                     RKEHFEHMVMGVLMSTHLSPEYLELEITESSAMENAEKAVDTLNCLSKLGLSIAIDDF
                     GTGYSSLAYLKRFPIQKLKIDRSFVKDIEGQNCDAAIAKSIIDLAHNMSLQVIAEGVE
                     CLDQSDWLLNRGCDQVQGFYYSRPLSEEQLMSFLNSDQVIRDITGVRLANIGRG"
     gene            1947..3029
                     /locus_tag="PKZ52_07905"
     CDS             1947..3029
                     /locus_tag="PKZ52_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59734.1"
                     /translation="MLAPAPHRVAPHAHSLLHLYLCDISLLTPLGAEIERVRAAVNAR
                     VSSYQACALPGDAEHSIMFSPIPDAALSCPLPLLLPGLSAPHIRLLKIAASTLGRLPF
                     INELHNSPLFLAGPELYYEKNRVSPLFIDHLIKTTGAAIEPKTSRYFASGRAGLLYAL
                     DAAFDYLEHNPKAVVLTGAMDTFYDAKTLGLLASQNRLLGEGVFDGFVPGEGATFLLL
                     TTAHGPTELINRAQLKLARPGLGFEPGHLLSNKPNPAHALSMAVGDALNTAAQPIKKI
                     YTSENGEAYYGREFSVPRLRHATKMIADVPVCRLAEFCGDLGAAFPGAALALAAQDAI
                     TSTADSILISASSDAGARAAICVSPV"
     gene            3070..3714
                     /locus_tag="PKZ52_07910"
     CDS             3070..3714
                     /locus_tag="PKZ52_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HNG59735.1"
                     /translation="MPRTVFANGRNFSHKGSGDQSICTVPDVCKTPVGSVTVPIPYPV
                     VSQVSNLADGTTSVKIDGNATAIESSTHSMCSGDEAGVAKGLLSGTTSSKTHFVTYSF
                     DVKADGKGVVRHMDMTTMNNRNTLGINFGSMSPAAMATTQEAKFEDIYTLRFEFVDEL
                     GQPVKGIPYKTLPAGQKDGWHLADNKTNGFGKTVMASSKKGESIDCFISWSKHE"
     gene            3724..4746
                     /locus_tag="PKZ52_07915"
     CDS             3724..4746
                     /locus_tag="PKZ52_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59736.1"
                     /translation="MADPIPVNGVNDTPGKTQQITIGCNELWSFAQQFSYLRLSDKID
                     ENHYKLIGLYAARARRIAATYARFYLETEEGGDKAKLGRYYWMALGAFASKTVACLLD
                     TLQLNASYALGKLTFETVDGHNVANGLGQGNLWLFSDIAPAHWFYSHYPTNFFNGMAC
                     LNKRDCDKLIEPVKSTVKGLPWAGKSIGKIKNFAPSESLKKGFNFVVQIESTTDKKLR
                     RSMQLEHLMAIADHEQKSVLQELIYKDPDFNKWTRFERKWWVHWAMPTYELIFSHACS
                     SGDDTLKSVAPDDLVVEDFTSRMQWITSAAKKFHGLMDGNAAYMHAELATISSWVNSP
                     DALLVY"
     gene            4817..5608
                     /locus_tag="PKZ52_07920"
     CDS             4817..5608
                     /locus_tag="PKZ52_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59737.1"
                     /translation="MYKPSISAKVGESGAAFLMRNGLSAEGNVDRQPAGMNFYQYRWK
                     TERAGEVVFDHGAFSFTIPNALTVMGTENLKHSGDGVESLFVNATFSSDKTITHDAAR
                     IALMKIIKELVSLGWKRYIAYSDPRLVGEQAFNHYLGNNFYSLPVDFVPNLQQWMQID
                     VGFWNLYADGVFLEIRFHRDSEHLDLNKPGAYLLVFNFETKTSYGRSMFDGEEREQWQ
                     EHWAELIKKAKKDRYAKEAELVKRGFTIDTTYQDPKIHPADPVEP"
     gene            5909..6700
                     /locus_tag="PKZ52_07925"
     CDS             5909..6700
                     /locus_tag="PKZ52_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59738.1"
                     /translation="MYKPKISVRLGQSGADFLAKNNLPAKGYINKQPAGVNFYEYDWS
                     VRHYGSVLVEHGPYSFNIPHALSVMGNEDVAKMQEGLSSFFICAGISGEETIYHDEAR
                     KIFYAFLHELVSQGWRYYTFYSEPRLTGFSAFRYYLENGIYTIPVDYMPTLDEWMHID
                     GGEWRLYAGDVFLQIRLLRDNTRIQLNAPGAYLLNFNLKSKDEFARYEFDGEERDHWQ
                     SLWVDKIKKAKKERYAKEAELVKRGFTIDTTYQDPKVHPADPVEP"
BASE COUNT         1882 a         1626 c         1829 g         1911 t
ORIGIN      
        1 gatgccggtg ttaatcgccc tcgcagacga caagggtaat ctgctgatgg ccagtgccca
       61 acacggcgag ctagagggcg tggcgggcaa ttgtgagcac tggttaaaat tggccgatgt
      121 gtaccccgcc agtttccaaa acgatttaga aagcctgttg gcggcgcaat ccaccagccc
      181 cgagtggttg gaatttaaag ccaaacataa agatggcagt tttcacgttt accaaattaa
      241 atatatttac catcaaagcg ccggccagtc cctgtgtttt gtcttcggca cggatattac
      301 cgcccataaa caaattgaaa attccctgcg ggatcataaa tcccgtttgg attatatggt
      361 ctaccacgac aatttaaccg gccttgccaa ccgcacctta ttttacggct gcatcaataa
      421 aaccctggaa ttcgcccgcg aaaaccacga aaccctgggt attttattgg tggatttaga
      481 ccgttttaaa aatgttaatg aaaacctagg gtacgacgca ggggatgacc tgctgcgtat
      541 tacggcggat aacctgcgcg cccagctgcg ggaaaaagac gtggtggcgc gcctaagcgg
      601 cgatgaattt gtggtggtgt tggatgatgt gcgccgcaaa agtgatttag aactggtggc
      661 gcaaaagctg ttaaaccgct tgagtttgcc ggtgaaatta cagggccact cgattatttc
      721 caccgccagt atcggcatta gtgtcttccc cgccgatggc gataatatcg accaattgct
      781 taaacacgcg gatttagcca tgcacaaggc taaagccaag ggcaaaaact gttttgaatt
      841 ttacgaagag gccctgcaca accaggctat taattattta ttgttggaaa atgatttgcg
      901 ccgggctata gaccagggcg aaattattct gcactaccag ccgcaaatta acctcaccaa
      961 tggccgcatt gtgggccttg aggccttggt gcgctggaac agcaaagagc gcggcatggt
     1021 gccgcccatg cattttatcc ccctggcgga ggagaccggt ttaattgagc ctttgggcga
     1081 atgggtattg cgccacgcct gcgagcgctt tcaagcctgg ttaatgaagg ggttaaaact
     1141 gggcaaaatg gcggtgaata tatcgccggt gcaatttcgc aaggagcatt ttgaacacat
     1201 ggtgatgggt gtattgatgt ccacccactt aagcccggaa tatttagagc tggaaataac
     1261 cgaaagctcc gccatggaaa atgctgaaaa ggccgtggat accttaaatt gtttaagtaa
     1321 attgggtttg tccatcgcta ttgatgattt tggcaccggc tattcgtctt tggcgtattt
     1381 aaagcgtttt cccattcaaa aattaaaaat cgaccgcagt tttgttaaag atatcgaagg
     1441 gcaaaattgt gatgcggcga ttgctaaatc cattatcgac ctggcccata atatgtccct
     1501 gcaggtgatt gccgaagggg tggaatgttt ggatcaatcc gattggctgc tcaatcgcgg
     1561 ttgcgaccag gtacaggggt tttattacag ccgcccatta agtgaagagc agttgatgag
     1621 ttttttaaac agcgaccagg tgatccgcga tattaccggg gtgcggttgg caaatattgg
     1681 gcgggggtaa ataattcaag attatgtaca ttgctattaa cccgctgagg cgggtttttt
     1741 gtttttaagc ctatatccgc acctatcaaa cgtgccgtga atactcagct gtcggctgag
     1801 tattgatggc tcacagcaaa gcacttgccc gatagtatcg agtcggcgtg agaatcccgt
     1861 ccagcaataa aaaggtgccg aatagttacg cttgttgtac catcgcgcgc cggtattttc
     1921 cctaattcac ctacaaggaa tccgcaatgc ttgcgcctgc gccgcaccgg gttgcgcccc
     1981 atgctcactc acttttgcac ctgtacctgt gtgatataag cctgctcacg cccttaggcg
     2041 ccgaaattga aagagtgcgc gccgctgtaa acgcgcgggt gagcagttat caggcctgtg
     2101 cattaccggg ggatgctgag cacagcatta tgttctcgcc cattccagat gctgccttgt
     2161 cctgcccgtt gccgttactt ttgcctgggc ttagcgcgcc gcatattcgc ctgttaaaaa
     2221 tagccgccag cactttaggg cggctgccat ttattaatga attgcacaat agccccttgt
     2281 ttttggcggg gccggaactc tattacgaga aaaatcgcgt aagcccgctt tttattgatc
     2341 acttaattaa aaccacgggc gctgcaatcg aacccaaaac aagccgctat tttgcatccg
     2401 gccgtgcagg gctgttgtac gcgctagatg cagcttttga ttatttagaa cacaacccca
     2461 aggctgttgt tttaaccggt gcaatggata cgttttacga tgccaaaacc ctcggcttat
     2521 tagcaagcca aaaccgccta ttaggggagg gcgtttttga tggttttgtg cctggtgaag
     2581 gcgccacatt tttgctgttg accaccgcgc acggccccac tgaattgatt aaccgcgcgc
     2641 aacttaaact tgcacgcccg ggccttgggt ttgaaccggg gcatttatta agtaataaac
     2701 ccaatccagc ccatgcgctt tccatggcgg tgggtgatgc actcaacaca gcggcgcagc
     2761 caattaaaaa gatttatacc tcagaaaatg gcgaagccta ttacggccgc gaatttagcg
     2821 tgccgcgcct gcgccacgcc actaaaatga tcgccgatgt acccgtgtgc cgtttggcag
     2881 agttttgtgg tgatttaggc gcggcatttc ccggcgccgc cttagccctt gccgcgcagg
     2941 atgcgataac atccacagcg gactctattt taatcagcgc atcatcagat gccggtgccc
     3001 gtgcagctat ttgtgtttca cccgtttaac gtttatttaa ttgctaatcc aatattgtta
     3061 ggaattttta tgccgcgaac tgtatttgcc aacggtcgaa atttcagcca caaaggcagt
     3121 ggcgatcaat ccatctgcac cgtgcccgat gtgtgtaaaa ccccggtcgg cagtgtaact
     3181 gtacctatcc cttatcctgt ggtaagccaa gtcagtaatt tggccgatgg caccaccagc
     3241 gtaaaaattg atggcaatgc aaccgccata gaaagttcca cccacagcat gtgttcgggt
     3301 gacgaagccg gagtggccaa agggctattg tccggcacca ccagcagcaa aacccatttt
     3361 gtaacctatt ccttcgatgt aaaagccgat ggcaagggcg tggtgcgcca tatggacatg
     3421 accaccatga ataaccgcaa taccctgggc attaatttcg gctccatgtc gcctgcggcc
     3481 atggcgacaa cacaagaggc gaaatttgag gacatttaca cactgcgctt tgaatttgtc
     3541 gatgaattag gccagccggt aaaaggcatt ccctacaaaa ccctacccgc cgggcaaaaa
     3601 gacggttggc acttggcgga caataaaacc aatggttttg gcaaaaccgt tatggcatcc
     3661 agtaaaaaag gcgaaagcat cgactgcttc atctcttggt caaaacacga ataaggaact
     3721 gatatggccg acccaattcc cgtcaatggc gtcaacgata caccgggtaa aacccaacaa
     3781 attactatcg gctgtaatga attatggtcg tttgcgcaac aattttccta tttacggctc
     3841 tccgataaaa tcgatgaaaa ccactacaaa ttaatcggcc tgtatgccgc acgggcacgc
     3901 cgtattgccg ccacctacgc gcggttttac ctggaaactg aagaaggcgg cgataaagcc
     3961 aaattgggcc gctattattg gatggcgctg ggggcgtttg ccagcaaaac agtggcctgt
     4021 ttattagaca cactgcaatt aaacgcctcc tacgcactcg gcaaactcac atttgagacg
     4081 gtggacgggc acaatgtagc caatggttta gggcagggaa atttatggct gtttagcgat
     4141 attgccccgg cccactggtt ctacagccat taccccacca atttttttaa cggtatggcg
     4201 tgtttgaata aacgggactg cgataaatta attgaaccgg tgaaaagcac ggtgaaagga
     4261 ctgccttggg cggggaagtc gattggtaaa atcaaaaatt ttgcaccttc ggaatctttg
     4321 aagaaagggt ttaattttgt tgtgcagatt gaatcgacta ctgataaaaa gctgcgtaga
     4381 agtatgcaat tggagcactt aatggctatt gcagaccacg agcaaaagtc tgttttgcag
     4441 gagcttatct ataaggatcc tgactttaat aaatggaccc gatttgagcg aaaatggtgg
     4501 gttcattggg caatgcccac ctacgagctc atcttttcgc acgcctgctc atcaggtgac
     4561 gataccttaa agtctgtagc gccggatgat ttggtggtag aagattttac cagtcgtatg
     4621 cagtggatta cgtctgccgc taagaagttt cacggcttaa tggatggtaa tgccgcctat
     4681 atgcacgccg aattagctac cattagtagc tgggtcaatt cgcccgatgc gcttttggtt
     4741 tattaaaatg actaaaataa tccttgcttg gctgctgttg cttacagcct gccattctga
     4801 acacgcggag aaagttatgt ataaacccag tatttcagct aaggtgggtg aaagtggtgc
     4861 cgcattttta atgcgcaatg ggctttcggc cgaggggaat gttgataggc agcctgcagg
     4921 gatgaatttt tatcaatacc gctggaagac tgaacgagcg ggtgaggttg tttttgacca
     4981 tggagctttt agtttcacaa ttcccaatgc cctaaccgta atggggacgg agaacttaaa
     5041 acattcgggc gatggggtcg aatctctttt tgtgaatgcg accttctcca gtgacaaaac
     5101 tattacccat gatgccgcca gaattgcgtt gatgaaaata attaaggagc tggttagttt
     5161 agggtggaag cgctacatcg cttattcaga tcctcggtta gtcggggaac aggcatttaa
     5221 tcattatttg ggtaacaact tttattcttt acctgtcgat tttgtaccca atttgcagca
     5281 gtggatgcag atcgacgtgg ggttctggaa tttatatgct gacggggttt ttttagaaat
     5341 tcgatttcat cgtgatagcg aacatctcga tttgaataag cccggcgctt atctactggt
     5401 ttttaacttt gaaacgaaaa cctcctatgg gaggagcatg tttgatggtg aagaacggga
     5461 gcagtggcag gagcattggg ctgagttaat taaaaaagcc aaaaaagatc gctacgcaaa
     5521 agaagcagaa ttagttaagc gcggttttac aatcgacacg acctaccagg atcccaaaat
     5581 ccatcctgcc gacccggtgg agccttaaaa ccactgttat ttctcgcatt tcatattttg
     5641 cttgctagat aagcgcagcg gttgcgccgg gacaccttgc gggcgaacaa attaataatg
     5701 gtttgataga ttgagtggtt tgcgaaagag gtggaacttt cgtatgggga gatgggtaaa
     5761 taattaagtt atgggtatgc tgaaatggtt ttgttggcca agggattggc ttgctataac
     5821 gcataactgg aatatttgca tgtataaatt gtttttgatc ataatttcat ttctggtagc
     5881 ctgccattct gaacggtcgg aggaggttat gtataagccg aaaattagtg taaggctagg
     5941 gcagagtggg gcggattttt tggcgaaaaa taatctgcct gccaagggtt atattaacaa
     6001 gcagcctgcg ggcgtgaatt tttatgaata tgattggagt gttcggcact acggcagtgt
     6061 tttggttgag catggccctt atagctttaa cattccgcat gcgctaagtg taatggggaa
     6121 tgaagatgtt gcaaaaatgc aagagggatt aagctcattt ttcatttgtg ctggcatttc
     6181 aggtgaggaa actatttatc atgatgaagc gcgcaaaata ttctatgcgt ttttgcatga
     6241 attggtgagc caggggtgga ggtattacac gttttattcg gagccgcgtt taactgggtt
     6301 ttcagctttt cgttattatc tggaaaacgg aatttacact attcccgtcg attatatgcc
     6361 cacgttagat gagtggatgc atattgatgg gggtgagtgg aggctttatg caggtgatgt
     6421 ttttttgcaa attcgcctgt tgcgggataa tacccgtatt cagcttaatg cacccggtgc
     6481 ctacttgttg aattttaatt taaagagcaa agacgaattc gctcgctatg aatttgacgg
     6541 cgaagagcgc gatcattggc aatcgctgtg ggttgataaa attaaaaaag ccaaaaaaga
     6601 acgctacgca aaagaggcgg aattagttaa gcgaggtttt acaatcgata caacctacca
     6661 agaccccaaa gtccatcctg ccgacccggt ggagccttaa gccaataggt attttcgcag
     6721 tgcatttttc acacgcgcga gcgccctagc gattgcgccc agatgatgtg ataggtattt
     6781 cgctacaagt agtacctaaa attcccctcc cttggagggg tgccccgtag gggcggggtg
     6841 gtgagccagg tgatattttt tattcgcaac ctgcgcttaa ttaagtaaaa aaattccgta
     6901 cacacggcag gtgcacctgc cacctcttca ggggcaatat cattaagcta atacaaagtc
     6961 ggaagggcaa tccttgtggt tgccctgcct gtacctcggc tattgaacag gatgtccact
     7021 gaagcgggca cccacaaggg gcgcccctac gcctgttggc gagccagtac atccttaact
     7081 caatggcatt agtgccatta agttaaggat tgccagccaa ttttgtagtc tggatggagc
     7141 ctagcgcaat ccagggtgcc tcgaaaatcc cggattgcgc taggctccat ccgggctacc
     7201 caggtattgc ttacgccaaa tcgcagccct ttttttaatt taatgaca
//
LOCUS       DAOQGS010000165         7247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5020_length_7247_cov_4.251669, whole genome shotgun sequence.
ACCESSION   DAOQGS010000165 DAOQGS010000000
VERSION     DAOQGS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5020_length_7247_cov_4.251669"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..940)
                     /locus_tag="PKZ52_07930"
     CDS             complement(194..940)
                     /locus_tag="PKZ52_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HNG59739.1"
                     /translation="MGSESDSVDLTPVADCRTCRFRTNLLLTGRCTPGDACVAVDSGR
                     QIDRFFRANPELAGNYINDGFWERRAVAARYIPQEALSRLIGDPDEAVRRAVAYRLPR
                     EQLLKLMDDPDREVRITVADRLPLEQLERMAADSDYLVRAYVAQRLPVGRLFRFIRDP
                     DRQVRKFVAQRLPEESLGLMVHDFEPEVRRIVASRLHGDAIVLLLSDSDWTVRLAAIP
                     NAPLEALNALLEDADPEVRQAAAERLAEGK"
     gene            complement(1114..1392)
                     /locus_tag="PKZ52_07935"
     CDS             complement(1114..1392)
                     /locus_tag="PKZ52_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HNG59740.1"
                     /translation="MAELTGLNKTQIDAVVTFVETNGLSDETVQLLRQQFSGCHFTYC
                     MDDDVHFSKAYAERAGFNLYLVDSRQHCSELTTDSDCASGIVLAEVIE"
     gene            complement(1401..2108)
                     /locus_tag="PKZ52_07940"
     CDS             complement(1401..2108)
                     /locus_tag="PKZ52_07940"
                     /inference="COORDINATES: protein motif:HMM:NF028154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     N-terminal domain-containing protein"
                     /protein_id="HNG59741.1"
                     /translation="MQHPQLTDEVALRIGLAVRALAVVDLRLLVNLLVSLVGHPITVE
                     KLSRLRLNRLKSAGGEVFDKTSEQDLRKALALLKGKGIEIAPDPQPIINAYSDGDMPG
                     SIRVAVASDRGEKINGAFGSCPRFLIYQVSAQEVRLIAVREVGKLVSDDDKLGYRAKL
                     IADCHVLYTTQIGGPAAAKVVRAGVHPMKVDAPVGARVLIRQLQAVLDTSPPPWLAKA
                     MGEEPNVRARFSLTQYG"
     gene            complement(2110..2328)
                     /gene="nifT"
                     /locus_tag="PKZ52_07945"
     CDS             complement(2110..2328)
                     /gene="nifT"
                     /locus_tag="PKZ52_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HNG59742.1"
                     /translation="MPNVMIRKNEVGQLTLYIAKQDLEENVVSVEFDTPERWGGEFTL
                     ADGSVYSVEPIARPELPITLRAKRGGES"
     gene            complement(2597..4168)
                     /gene="nifK"
                     /locus_tag="PKZ52_07950"
     CDS             complement(2597..4168)
                     /gene="nifK"
                     /locus_tag="PKZ52_07950"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HNG59743.1"
                     /translation="MSQNVDNIKPSYPLFREDDYKETLRNKRAEFEEVHPQTKIDETF
                     LWTTTTEYQELNFQREALTVNPAKACQPLGAVLCALGFEKTMPYVHGSQGCVAYFRTY
                     FNRHFKEPISCVSDSMTEDAAVFGGQQNMKDGLQNCKATYKPDIIAVSTTCMAEVIGD
                     DLNAFINNSKKEGYIPAEYPVPFAHTPSFVGSHVTGWDNMFEGMVKYFTINDMEGKVV
                     GSNHKINLVPGFETYLGNFRVIKRMLKEMDVGYTLLSDPEEVLDTPADGEFRMYSGGT
                     TQAEIKDAPNAIDTLLLQPWQLEKTKKFVEQTWKHPAAKINVPMGLDWTDEFLMKVSE
                     ITGQPIPDSLAKERGRLVDMMTDSHTWLHGKRYALWGDPDFVIGMVKFLLELGCEPTH
                     ILCNNGNKRWKKAVEAILAESTYGKNCTVHIGKDLWHMRSLVFTDKPDYMIGNSYGKF
                     IQRDTLHKGKEFEVPLIRIGFPIFDRHHLHRQTTFGYEGAMQILTTLVNTELERLDDA
                     TRGMQTTDYNYDLIR"
     gene            complement(4290..5771)
                     /gene="nifD"
                     /locus_tag="PKZ52_07955"
     CDS             complement(4290..5771)
                     /gene="nifD"
                     /locus_tag="PKZ52_07955"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HNG59744.1"
                     /translation="MSGMSREEVESLIQEVLEVYPEKAKKDRAKHLAPNNPELDQAKK
                     CITSNKKSLPGVMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYIG
                     TTGVNAFVTMNFTSDFQEKDIVFGGDKKLAKLIDEIETLFPLNKGISIQSECPIGLIG
                     DDIEAVAKKKQAEHGKVVVPVRCEGFRGVSQSLGHHIANDAVRDWVIGNRDGDDSFAT
                     TPYDIAIIGDYNIGGDAWSSRILFEEMGLRVVAQWSGDGTISEMELTPKVKLNLVHCY
                     RSMNYISRHMEEKYKIPWLEYNFFGPTKTVESLRKIAAHFDESIQAKCEEVIAKYKPE
                     WDAVIAKFRPRLEGKRVMLYIGGLRPRHVIGAYEDLGMEVIGTGYEFGHNDDYDRTLK
                     EMGDSTLLYDDVTGYEFEEFVKKLKPDLIGSGIKEKYIFQKMGVPFRQMHSWDYSGPY
                     HGFDGFAIFARDMDMTLNNPCWKKQQAPWKQAEASHTAIAASA"
     gene            complement(5942..6820)
                     /gene="nifH"
                     /locus_tag="PKZ52_07960"
     CDS             complement(5942..6820)
                     /gene="nifH"
                     /locus_tag="PKZ52_07960"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HNG59745.1"
                     /translation="MAMRQCAIYGKGGIGKSTTTQNLVAALAEAGKKVMIVGCDPKAD
                     STRLILHEKAQNTIMEMAAEAGTVEDLELEDVMKVGYGDIRCVESGGPEPGVGCAGRG
                     VITAINFLEEEGAYEGNLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMY
                     AANNISKGIVKYANSGSVRLAGLICNSRNTDREDELIMALADQLGTQMIHFIPRDNVV
                     QRAEIRRMTVIEYDPKAKQADEYRALANKIVNNTKFVIPTPITMDALEKLLMDFGIMD
                     EEDLSIVGKSAAEELA"
BASE COUNT         1958 a         1786 c         1600 g         1903 t
ORIGIN      
        1 gcctacacgg atgtattcac ggcgtgtctg cactaacctg tagtcaccac tgcggcgctg
       61 gattacggag tcacagcact aaaacgcgcg cgatagttcc cagtcaacgc aggaattacc
      121 ttcagccaca taaaaagatg ctggacagtt acgatttttc aagcattaag acctgacgag
      181 aaacagaaga aaattattta ccttccgcca accgctccgc cgctgcctgg cgcacttcag
      241 ggtcggcatc ttccagcagt gcatttaatg cttccaaagg tgcattgggg attgccgcta
      301 agcgcactgt ccaatcgcta tcgctgagca agagtactat ggcatcgcca tgcaaacggg
      361 aggcgacgat tctgcgcacc tcgggctcga agtcgtgcac cattaatcct aaggattctt
      421 ccggcaaacg ctgagcgacg aatttacgca cctgccgatc cgggtcgcga ataaaccgaa
      481 ataatcgccc gacaggtaac cgctgcgcaa cataggcacg cactaaataa tcggaatccg
      541 ccgccatgcg ttcaagctgt tctaacggca aacgatcagc caccgtaatg cgcacttctc
      601 gatccgggtc gtccattaat tttaataatt gctccctagg taatcgataa gccaccgcac
      661 ggcgcactgc ttcatccggg tcgccaatta atcggcttaa ggcctcctgg ggaatataac
      721 gggccgccac cgcgcggcgc tcccaaaagc catcattgat ataattgccc gccaattcag
      781 gattcgcgcg gaaaaaacgg tctatttgcc gcccgctgtc cacggcaaca caagcatccc
      841 caggtgtgca gcggccagtt aataataaat tggtgcgaaa gcggcaggtg cggcaatcgg
      901 caaccggggt taaatccact gaatccgatt cactacccat gataaaaccc cttgcacacc
      961 tgatattgac gccaaaaata aaaacattac cgcagcttta ggccgcagga ttttaactaa
     1021 aaatcttttt tataaaacca aaagaaatcc taggcgcacc aggttaaatc ctgcctcaac
     1081 ctacaggctc ccagagcgac acaactttct agcctattca attacttccg ccagtacaat
     1141 tcccgatgca caatcagaat ctgtagtaag ttcagagcag tgttggcgag aatccactaa
     1201 atagagatta aacccggcac gctcagcata ggctttggaa aaatgcacat catcatccat
     1261 gcagtaggta aaatggcaac cgctaaattg ctggcgcagt aattgtactg tttcatcact
     1321 taaaccattg gtttccacaa atgtaacgac tgcgtcaatt tgtgttttat taaggccagt
     1381 tagctctgcc ataaaaacct ctaaccatac tgagtcagcg aaaaacgcgc gcgcacattg
     1441 ggctcttcac ccatagcctt agcaagccat ggcggtggcg atgtatccag taccgcttgc
     1501 aattggcgaa ttaaaacccg agcccctaca ggggcgtcta ccttcatagg gtgcacaccg
     1561 gcacgcacca cttttgcggc ggccggccca cctatctggg tggtgtataa cacatggcaa
     1621 tcggcaatta attttgccct gtagccgagt ttatcatcgt cacttactaa tttgcccact
     1681 tcccgtaccg caattaaacg cacttcctgg gcggacacct gataaattaa aaaacgcggg
     1741 caactaccaa aagcgccatt tattttttca ccgcgatcac tggcaaccgc cacacgaata
     1801 gaacccggca tatcgccgtc actataggca ttaattattg gctgaggatc cggagcaatc
     1861 tcaatgcctt tgccttttaa caaggccagg gctttacgca aatcctgttc ggatgtttta
     1921 tcaaacacct cgccaccggc agattttaag cggtttaaac gcaagcgcga tagtttttca
     1981 accgtaatag gatgccctac taggctcacc aaaagattaa ctaaaaggcg caaatccaca
     2041 accgctaatg cgcgcacggc aagaccaatc cgtagagcga cttcatcagt taattgtgga
     2101 tgctgcatat taggattctc caccgcgttt agcgcgcagt gtgataggta attccggccg
     2161 agctatgggc tcaactgaat aaactgaacc atcggcaagg gtaaattctc cgccccaccg
     2221 ctcaggggta tcaaattcaa cggagacgac attttcttct aaatcttgct tagcaatgta
     2281 aagcgttaac tgaccgactt catttttgcg gatcatcaca ttgggcataa cacattcctg
     2341 actcatcaga cgagaaaaaa cggcggtagc aaatagttgg cagccactac cgcagtgcga
     2401 gttgacgcaa aaggcgtttt cagatcaata actttaacaa cttaaaaccc acggggtttg
     2461 atacatatta agtttggggc ttttaaaaat acccggtgcg aaattcgcac cgggccaatc
     2521 aacacaacaa acacacaaca gaaactagtt ggcaagcttg ttaattacac caccaaacaa
     2581 ctgggcaaat tgcctattaa cggatcaaat cgtagttata gtcggtagtc tgcataccac
     2641 gggtagcatc atccaagcgc tcaagttcag tattaacaag agtggttaga atctgcatag
     2701 caccctcgta accaaacgtg gtttggcggt gcagatggtg acggtcaaag atggggaatc
     2761 cgatgcgaat taatggcact tcgaattctt tacccttgtg caatgtatcc cgttggataa
     2821 atttaccgta ggagttgccg atcatgtaat cgggtttatc ggtaaatacc aaagagcgca
     2881 tatgccacag atctttgccg atgtggacag tgcagttttt gccataggta gattcagcca
     2941 aaatagcttc cacagctttt ttccaacgct tgttgccgtt gttgcacaag atgtgggtag
     3001 gctcacaacc tagttcaagc aggaatttaa ccatgccgat gacaaagtca gggtcacccc
     3061 agagtgcata acgcttgccg tgcaaccagg tgtgggagtc agtcatcata tccactaaac
     3121 gaccgcgctc tttggcgagg ctgtcaggaa ttggctggcc agtgatttcg ctgactttca
     3181 ttaagaattc gtcagtccaa tccaaaccca tgggcacgtt aattttagcg gctgggtgtt
     3241 tccaagtttg ttctacgaac ttcttggttt tttccaactg ccaaggttgc aacaacaaag
     3301 tgtcgatagc gttaggcgcg tctttaattt ccgcttgcgt ggtaccgcct gagtacatgc
     3361 ggaattcacc gtctgcaggc gtatccaaca cttcttcagg gtcagaaagc agtgtgtagc
     3421 ctacatccat ttctttaagc atacgcttaa ttacgcggaa attacccagg taggtttcaa
     3481 aaccgggcac taggttaatt ttgtggttgc tgccaaccac tttgccttcc atatcattga
     3541 tagtgaaata cttcaccatg ccttcaaaca tattgtccca gccggtcacg tggctgccca
     3601 cgaagcttgg tgtatgggca aacggcacag ggtattcagc aggaatataa ccttcttttt
     3661 tggagttatt gatgaatgcg tttaagtcat caccaattac ttccgccatg caggtagtgg
     3721 aaacggcaat aatgtcaggc ttgtaggtag ccttacagtt ttgtaagccg tctttcatat
     3781 tttgctggcc accaaatacg gctgcatctt cagtcatgga gtctgatacg caggagatag
     3841 gctctttaaa gtggcggtta aaataggtgc ggaaataagc cacacaacct tgcgaaccgt
     3901 gcacataagg catagttttt tcaaagccta aggcacacaa aacagcgcct aagggttggc
     3961 aggctttagc ggggttaacg gttaaggctt cgcgctgaaa attaagttct tgatattcag
     4021 tggtagttgt ccacaaaaat gtctcatcaa ttttggtttg cggatgaact tcctcaaact
     4081 cggcgcgttt gttacgaagg gtttctttat agtcgtcttc acgaaataaa ggataactgg
     4141 gtttaatgtt atcaacattc tggctcatga tattcctcct cccgcgccac caatctgtgg
     4201 acgaacgggt agtttgattg gatagcacag ccgttaaaaa aacggctgcg caggtttaca
     4261 acaggtatta ccccttaggg aaattcagct taagcgctcg ctgcaatggc agtgtgagaa
     4321 gcttcagctt gtttccaggg tgcttgctgt tttttccagc aggggttgtt cagagtcata
     4381 tccatatcgc gggcgaagat agcgaagcca tcaaagccgt ggtaagggcc tgaataatcc
     4441 caggagtgca tttgtctaaa aggcacaccc attttttgga aaatgtactt ttctttaata
     4501 cctgaaccaa tcaaatccgg cttgagcttt ttcacaaact cttcgaattc gtagccagtt
     4561 acgtcatcgt acaacagagt tgaatcgccc atttctttta aggtgcggtc gtagtcatcg
     4621 ttgtggccaa attcatagcc agttccgatt acttccatac ctaaatcttc ataggcacca
     4681 attacgtggc gagggcgcag accgccgatg tacagcatga cgcgcttgcc ttccaaacgt
     4741 gggcggaatt tagcaataac tgcatcccac tcgggtttgt atttggcaat cacttcttca
     4801 cattttgctt ggatcgactc atcaaaatga gcagcgattt tacgcagtga ctcaacggtt
     4861 ttggtagggc cgaagaagtt gtactctaac catggaattt tgtacttctc ttccatatgg
     4921 cgagagatat agttcattga acggtagcag tgcactaggt tcaattttac ttttggtgtc
     4981 aattccatct cagaaatggt gccgtcaccc gaccactgag ctacaacgcg caggcccatt
     5041 tcttcaaaca gaatacggct agaccaagca tcaccaccga tgttgtagtc accgataata
     5101 gctatgtcgt aaggcgtggt agcaaagcta tcatcaccat cacgattacc gataacccaa
     5161 tcgcgcacgg cgtcattagc aatgtggtgg cctagggatt gagacacacc gcgaaagcct
     5221 tcacaacgaa caggaacaac gactttgccg tgttcagctt gttttttctt ggcaaccgct
     5281 tcgatgtcat caccgatcaa accgattggg cactcagatt gaattgaaat acctttattc
     5341 agcgggaaca gggtttcaat ttcatcgatt aatttagcga gttttttgtc gccaccaaat
     5401 acgatgtctt tttcttggaa atctgaggtg aagttcatag ttacgaacgc atttacaccg
     5461 gtggtaccga tgtaatagtt gcgacgaccg gcacgagagt actgaccgca gccaactgga
     5521 ccatgggaga tatggatcat atctttaatg gggccccaca caacgccttt tgagcctgca
     5581 taggcgcaac cgcgaatagt cattacaccg ggtaatgatt ttttattcga ggtgatgcat
     5641 ttttttgctt gatctaattc tgggttattc ggcgccaagt gtttagcgcg gtcttttttt
     5701 gctttttcag gatagacttc aagtacttcc tgaatgaggg attccacctc ttctcgtgac
     5761 atgccagaca ttgtaatgtc tcctataatt gcatcaatta ccgggtaacg gtgttgccag
     5821 gcccttgcgc tcagccaaaa ggttaagagc ctagcaatac cggcagggct aatctgccct
     5881 gccccgaact ttttctagca atctacaagg caaagcctcg tacatttaag cgcgctgcaa
     5941 attaagccag ttcttcagcg gctgatttac caacgatgct taaatcttct tcatccataa
     6001 taccgaagtc catcaacaat ttttccaacg catccatagt gatgggtgtt ggaataacga
     6061 acttggtgtt atttacgatt ttgttagcca aagcgcggta ttcatcagct tgtttggctt
     6121 ttggatcgta ttcaataaca gtcatgcggc gaatttcagc gcgttgcaca acgttatcgc
     6181 gtgggatgaa gtgaatcatt tgagtaccca attgatcagc tagggccata atgagttcgt
     6241 cttcacggtc agtgttacgg ctgttgcaga tcaaaccagc caagcgcacg ctaccagagt
     6301 tagcgtattt cacgatgcct ttagaaatgt tgttggcagc gtacattgcc atcatttcac
     6361 cagagcatac gatgtaaatt tcttgcgctt tgttttcacg gattggcata gcaaatccgc
     6421 cgcacactac gtcgccgagt acgtcgtaga atacgaagtc caaattgcct tcgtaggcgc
     6481 cttcttcttc aaggaagttg atggctgtaa ttacaccgcg accggcgcaa cctacacctg
     6541 gctcaggacc acctgattct acacagcgaa tatcgccgta gccgactttc attacatcct
     6601 ctaactcaag atcttctacg gtgcctgctt cagcagccat ttccataata gtattttggg
     6661 ctttttcatg tagaattaaa cgagtggaat cggctttagg gtcacaacct acgatcatga
     6721 cttttttgcc cgcttctgct aaagcggcta ctaggttttg agtggtagtt gatttaccaa
     6781 taccaccttt accgtatatt gcacattgac gcattgccat ttgagttctc ctaaatttcg
     6841 aagcaattaa attgcttata aaaaggttcg gatgtaagca acgaccaaga gtttcaaggt
     6901 gcccttacac aaaactcaaa gtgcaaaggg cgggccattt ttattaagta ttttaatagc
     6961 ctgtttttta agtgtttttt agatcgagct ggccgcgaca cacgacaaac accgcaacaa
     7021 tccgcaagct atgtaaggtt gacaacaaac aattccctcc cacgccaaca aatcaccttc
     7081 gcctaaaaaa cccgacgaaa atgacttttg tcgtaaaccc gccaaaatga aggcaaccga
     7141 gcaggcaagc cagattgaag gtatgactgc agcaggttat ttagcctaag gccataccac
     7201 agggcttaag ctgaggtttt ggattaattc gattcttctt cccaact
//
LOCUS       DAOQGS010000166         7225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5045_length_7225_cov_6.474198, whole genome shotgun sequence.
ACCESSION   DAOQGS010000166 DAOQGS010000000
VERSION     DAOQGS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5045_length_7225_cov_6.474198"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>7225)
                     /locus_tag="PKZ52_07965"
     CDS             complement(<1..>7225)
                     /locus_tag="PKZ52_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59746.1"
                     /translation="TNGGDIKIGSSQVPIGGTVSAANATLNTSATNGAGDITIDAKGN
                     VSLGVLDLSRTFNSTATKAGSLTVTSQGSISFEKNLDFNNFAAYNGANSSNENWTLPN
                     NTPEADKKTIFTLNAAGDINFKGNIFDSYGDGRDGLNIVANADAEVMTGGKDGTGSVI
                     VGNTVYTSGGNVEFTGAGIQFGTSALIDTDRANSSPDNAVTGNGGYGNATRSDGGNVI
                     LSSDSNIVLGSITTDHFCTLGTCAGDLTIQNRSSDKSISITQAAGKALSVHGTTTLKT
                     NGGNITLGEAGNIFGTSGANAGTNSNDNNIDTLVNNAKVAAGKTIAITNAGSSTIKGD
                     NSYLTLGNVSAQGLSLSTTKDIRQFGGTSIDANTAAFKGTSVLLINNGNKLGTVTSLE
                     TTATSAGSASINVTQDITLSALNIASGQLGLSMAGGKSATFDGAITANSAFTVTASGA
                     NTTANLADSLSKTGGSSVTFAGFNTLHYTGANEASWNLTSSAQTVSWSGKTFKFDTAA
                     SLVGGGGVDKFALSGTPFSLPTLDGAGGDDELTLDASNATTSKNWSVELGSSSAAAEG
                     KIRATHIESLTVTNTPPNVAVQLSIGNTKNTSWNLTGASAIFTLNNEDVGEIDFTGFN
                     QLTGGDGKDTFDYSNGGSQVFAGKIDGAGGTEDTIKGFNADTSWDLTATPASFYKTAD
                     SSTKYNFSGVEKLVGGSGKDTVTLAASVTEVNLNAFSTGFSLENFEGITATAAGGAKL
                     TGYSGNSTWEITKANEGKITSGAQSTEFVGFSELISSNAQNNKDEFTVTSTGSVTSIT
                     GQADDTLNARSGTTITNNWTIAADGSAQVKEGATTYINKISGVGTVNASTGADNFTAV
                     GTYGGTIKATQGNAAEDVNALSTAKADVVFAATISANGIEGITDFKADGTNAELKASS
                     AAGAVNWAVTPGAAAGQLSGKVTDATSGKNLTFSDFATLVGGDNADNFTLGAGTFTGG
                     ALQGGNGANKLINTVTNTSWDLDTGKLRTVQFTGIQTLQGASGDSLKAANASNTFTLD
                     NTSTRLASQDTNNLVTNYTLENFTTLVGGDNQDTFKVDIDSANVELQGGNGNDTFTLA
                     SETIKTKNILGGDGSDTLSVSTGTNNWVLTAANTGTVNANTFSGVEILAGGAGSDSFE
                     NKIAGQTLSLTAIDAGSLHASGDETNIAITLSGMNSVNKLAALKGFSGTTTWALNTDG
                     AGTVKTATNASLSFTEVTQIIGGSGDETFTVDKAVTAFNIDGSTGVNTLQGPQLVNTW
                     KLTAQTAAVGNQPAANTGTLNGAGSFAGMQILKGGSLADGLDLTGSTGLTVALANITG
                     FEDLIGGTNSTLTASNQTNSWQLNAGTSGLINSITFKDFALLNGGTGQDIFAIANSSQ
                     FKGEIEGGLNTSGSVLDELKIDSVAGKFVVSAQGAGTLQYINTTNSTTTGVTFRGIEA
                     LTGGSAADTFDVTTKVEMNLNGGIGKDAIKLPNEDIDISLAVGSGVPSVGSLTLSNFE
                     NIEAGATTNSTLRAVDGEATWAITDKNSGTLTLTKNAQTSETEFNNFNKLVSGSGVDH
                     FIFGDKGSIKTINANDGDDLLGRNAATRWDLTTTLQLTDTSATATVYAESISGLKKWQ
                     GGVGADDFNLSAATTGLNNLSIDGGDGQNQFTSPSGSWLWALSGSQQGTLALSGQTIS
                     FTQVNNLIASANQTLTNSAAVDAWKINQANQLTAVIGLEATNLNQFNLVNTGAGNDVL
                     EINKAYSGSINLQGGTNSVTLGAAGTIAGLSAGSGTDKIKSAYASSVWTQTEENNKRF
                     IKVAQGNTSLIGELTGFESIEVAGAHTLATTLTDNNWAIADSVSGTLNGIGFSGFKTL
                     LGSDENDTFSFANGAAFGGTIDGGKGNNLVDVSTGSQTKYELNEAQGSEINGVKNATG
                     VKGAGSSAQLTLVSPNKASWSITGDNKGELTYTLLNKTFSFEGFGVLVGGSGDDTFVF
                     SSPTAKINRIEGGAQALNGVDTVVAKSNQANLWTVGAGNNLDNTLAAANGTAYLNDYD
                     GIETITGAGQDKLVIAAGSTQWELNKNTGNLINPPAVTGASSIALSFSGINTLWGSDG
                     KDTYNLIEVPEFADIDGRGGDNLVQLAAQNAVLDLTQGFAVQGVKNVTGVSGSGAAAS
                     LALVANSNATWTLTDKNAGSIKVTISGQDKSIAFDNFVNLTGGSNNDAFILDTKGYVT
                     GTINGGGTSNGGQNSLTAWSGENANWILDASSSVTGVSSALKQSFSGITLLNGSDKND
                     LFTLRSAQAFTINAGLGNDTLRLNTDSRLIGQFNGGEGVNTVQTIDGQNTWQLNSANS
                     GAVLITGETTSRASFTQAGNLIGGSGVDNLIGFDTTATWDVTKGEIATGVLGADKTTF
                     SKMEALTGGNQSDVFKVSSAFAGDIFAGGGSDTLELLANGSVAS"
BASE COUNT         1738 a         1862 c         1635 g         1990 t
ORIGIN      
        1 aactggccac cgaaccattg gctaataatt ccaaagtatc gctgccgcca ccggcgaaaa
       61 tatccccggc aaatgcgcta cttactttaa atacatcgct ctggttgccg ccggtaagtg
      121 cctccatttt gctgaaggtc gttttatccg cccccaacac acccgtggcg atttcaccct
      181 tggtaacatc ccaagtagct gtagtatcaa agcctattaa attatcaacg ccgctgccgc
      241 cgattaaatt gcccgcttga gtaaagcttg cgcggctggt ggtttcaccc gtaatcaata
      301 cagcgccgct gttggccgaa tttaattgcc aggtgttttg tccatcgatg gtttgtaccg
      361 tattaacacc ctcgccgccg ttaaattggc caattaaacg gctatcagtg ttaagcctta
      421 aggtgtcatt gccaaggcct gcattaatgg tgaacgcttg tgcagagcgc agcgtaaata
      481 ggtcgttttt atcgctgccg ttaagcagcg tgataccgct aaaactttgt tttaatgcgc
      541 tgctaactcc cgtcacactg gagctagcat ctaaaatcca gttggcattt tcaccgctcc
      601 aagcggtgag gctattttgc ccaccattgc tagtgccacc gccgttaatc gtaccggtga
      661 cataaccctt ggtatccaaa ataaaggcat cgttattact gccaccggtt aaattaacaa
      721 aattatcaaa ggcgatggat ttatcctggc cgctgatcgt gaccttaatt gagcctgcgt
      781 ttttatcggt caatgtccag gtggcattac tattggccac caaggcaagg ctggctgcag
      841 caccgctgcc ggatacaccc gtgacatttt ttacgccctg cacggcaaac ccttgggtta
      901 aatccaatac cgcattttgc gccgccaatt gcaccaggtt atcgccgccg cgcccatcga
      961 tatcggcgaa ttcaggcacc tcaataaggt tataggtatc tttgccgtcc gaaccccaca
     1021 gggtattaat gcccgaaaaa ctcagcgcaa tactgctggc accggtgacg gcggggggat
     1081 taattaaatt gccggtattt ttatttaatt cccactgggt gctgcccgca gcaatcacta
     1141 atttatcctg cccagcgccg gtaatggttt caatgccgtc gtaatcattt aaatacgcag
     1201 tgccattggc ggcggcgagt gtattatcca ggttattgcc cgcgcccacc gtccacagat
     1261 tcgcctgatt acttttggca acaactgtat cgacaccatt taacgcctgc gcgccgcctt
     1321 cgatgcgatt tattttggcg gttggcgagg aaaagacaaa cgtatcgtcg ccgctgccac
     1381 ccactaacac accaaagcct tcaaagctga aggttttatt caataatgtg taggttaatt
     1441 cacctttgtt gtccccagta atactccaac tggctttatt gggcgacacc aaggttaact
     1501 gcgccgaact gcccgcacct tttacaccag tggcattttt aacgccgttt atttcactgc
     1561 cttgggcttc atttaattca tatttggttt ggctgccggt ggaaacatcc accaggttat
     1621 tacccttgcc gccatcaatt gtgccgccga aggcagcgcc gttggcaaat gaaaaggtgt
     1681 cattttcatc agagcctaac agggttttaa acccgctaaa gcctatgcca ttgagtgtgc
     1741 cacttacact atcggcaatg gcccaattat tgtcagtaag cgttgtagcc agggtatggg
     1801 cgcccgccac ttcaatggat tcaaagccag ttaattcacc gatcaggctg gtattgcctt
     1861 gggcaacttt tataaagcgt ttgttatttt cttccgtttg ggtccaaacg ctggacgcat
     1921 aagcgctctt aattttatcc gtgccgctac cagcactcag gcccgcaata gtgcctgcgg
     1981 cgccgagggt tacgctgttg gtgccgcctt gcaaattaat actgccgcta taggccttat
     2041 taatttccag cacatcattc ccagcacctg tgttgacaag gttaaactgg tttaaattgg
     2101 tagcttcaag gcctattacc gccgtgagct gattggcttg attaattttc caggcgtcca
     2161 cggctgcgga gttagtcagg gtttgattag cgctggcgat taaattattt acctgagtaa
     2221 aactgatagt ttgcccgctt aaggctaaag tcccctgctg gctgcccgat aaggcccaca
     2281 gccaactgcc gctggggcta gtgaattgat tttggccatc gccgccatca atacttaaat
     2341 tattcaagcc cgtagtggca gcggataaat taaaatcatc cgcccccaca ccgccctgcc
     2401 attttttaag gccgctaata gactcagcat aaactgtggc agtcgcgctg gtatcggtta
     2461 attgcagtgt ggtggtgagg tcccagcgag tagccgcatt gcggcccaac agatcatcgc
     2521 catcgttggc attaatggtt ttaatactgc ctttatcgcc aaaaataaaa tgatccacac
     2581 cgctgccgga aaccaattta ttaaagttgt taaattcggt ctctgaggtt tgggcatttt
     2641 tggtaagggt cagcgtgccc gaatttttat ccgtgatcgc ccaggtggct tcgccatcta
     2701 cagcgcgcaa cgtgctattg gtagtggcac cggcttcaat attctcaaaa ttcgacagcg
     2761 taaggctgcc aacgcttggc acccctgaac ccaccgccaa ggaaatatcg atatcctcgt
     2821 ttggcagctt aatggcatct ttgcctatac cgccatttag gttcatttcc actttcgtgg
     2881 tgacatcaaa tgtatccgct gcgctgccgc cggtaagcgc ctcgataccc cgaaatgtca
     2941 cgcccgttgt ggtgctattg gtagtattaa tatattgcaa ggtgccagca ccttgcgcgg
     3001 atacgacaaa tttacccgcc accgaatcaa tctttaattc atccagcacg ctgccagaag
     3061 tattaagccc accttcgatt tcacccttaa attgtgagct attggcgatg gcaaaaatat
     3121 cctgccctgt gccgccattg agcagcgcaa aatctttaaa ggtgatggaa ttaatcagcc
     3181 cgctggtacc ggcatttaat tgccaactgt tggtttgatt gctggccgtc aaggtcgaat
     3241 tggtgccgcc aattaaatct tcaaacccgg ttatattcgc aagtgcaacc gttaatccgg
     3301 tgctgccggt taaatctaaa ccatcggcta aagaaccacc ttttaatatc tgcatgcccg
     3361 caaaggaacc tgcgccattg agagtaccgg tattggcagc gggttgattg cctacagcgg
     3421 ccgtttgggc ggttagtttc caagtattaa ccagctgcgg cccctgcaat gtgttaacac
     3481 ctgtactgcc atcaatatta aatgcagtta cagccttatc aacggtaaag gtttcatcgc
     3541 cgctgccgcc gataatttgt gttacctcag taaaactaag gctggcgtta gtggcggttt
     3601 taaccgtacc ggctccatcg gtattgagcg cccaagtagt ggtgccacta aaacctttta
     3661 aagcggccag cttattaacg ctattcatac cgctaagggt aatagcaata ttagtctcat
     3721 cgccgctggc atgcaggcta cccgcatcaa tggccgtaag gctcagggtt tgcccggcaa
     3781 ttttattctc aaaactatcg ctgcctgcac caccggctaa aatctccacg ccgctaaagg
     3841 tattagcatt cactgtacct gtattggccg cggttaaaac ccaattatta gtgccagtac
     3901 ttacgctaag tgtgtcgcta ccgtcaccgc ccagtatatt tttggttttt atagtttcac
     3961 tggccaaggt aaaagtgtca ttgccgttac caccttgtaa ttccacattg gcagaatcaa
     4021 tatcaacctt aaatgtatcc tggttatcgc cccccaccaa agtggtgaaa ttttccaatg
     4081 tgtagttggt aaccaggtta ttagtatctt ggcttgcaag gcgtgtactg gtgttatcca
     4141 gggtaaatgt attgctggca ttagccgcct ttaggctatc accgctggcg ccttgcaggg
     4201 tttgtatgcc cgtaaactgc acggtgcgca gtttaccggt atctaaatcc cagcttgtat
     4261 tggtgactgt attgattaat ttattagcgc cattgccacc ctgcaacgca ccgcccgtaa
     4321 atgtacctgc gccaagggta aaattatcgg cattatcacc acccaccagg gtggcaaaat
     4381 cactaaaggt taggtttttg ccagacgttg catcggtaac cttaccggat aattgccctg
     4441 ctgccgcacc gggtgttacg gcccaattaa ccgcaccggc agcagacgag gcttttaatt
     4501 cggcgttagt gccgtcggct ttaaaatcag taataccctc aatgccattg gcgctaatcg
     4561 tagcggcaaa tacaacatcg gctttggccg ttgacagcgc atttacatct tcagcggcat
     4621 tgccttgggt tgctttaatg gtgccgccat aagtgcctac agcggtaaaa ttatccgccc
     4681 cagtactggc attaaccgtg cccacgccgc ttattttatt aatataagtg gtggcgccct
     4741 ctttaacctg ggcgctaccg tcagcagcaa tggtccaatt attggtaatg gtggtgccgc
     4801 tgcgcgcatt gagcgtgtcg tccgcttggc ccgttatgga ggttacagag ccggtggatg
     4861 ttacggtaaa ttcatcttta ttattttgtg cattggaact aatcaactcg ctaaaaccca
     4921 caaattccgt ggattgcgca ccgctggtaa ttttaccttc gttagcctta gtgatttccc
     4981 aagtggaatt accgctgtag ccagttaact ttgcgccgcc tgcagcagtt gccgtaatac
     5041 cctcaaaatt ctccaacgaa aatcccgtgg aaaacgcatt cagattcact tccgttacgc
     5101 ttgcggcaag cgtgactgta tctttcccgg aaccgcccac cagtttttct acgccactaa
     5161 aattatattt agtactgcta tcggcggttt tataaaaact cgcaggtgtt gcggttaaat
     5221 cccaggaggt atcggcatta aaacccttaa tggtatcctc agtacccccc gcaccatcaa
     5281 ttttgcctgc aaatacctgg ctgccgccat tggagtaatc aaaggtatcc ttgccatcac
     5341 cacctgtaag ctggttaaag ccggtaaaat ctatctcgcc cacatcttca ttattcagcg
     5401 taaatattgc agatgcgccg gttagattcc aggacgtatt ttttgtgtta ccaatactca
     5461 actgcacagc aacattaggc ggcgtatttg taaccgtaag tgattctata tgtgttgcgc
     5521 gaattttccc ttctgcggcg gcacttgatg agcctaattc cactgaccag tttttgctgg
     5581 tggtggcatt gctggcatct aaagttaatt cgtcatcgcc ccctgcgcca tctaaagtag
     5641 gcaggctaaa aggagtgccg gagagtgcga atttatcaac accaccgccc cccacaagcg
     5701 aggctgccgt atcaaattta aacgttttac cgctccagct cactgtctgg gcgctagatg
     5761 tcaaattcca actcgcctcg ttggcgcccg tgtaatgcag cgtattaaac ccagcaaatg
     5821 tcacgctgct gccgcccgtt ttactcaagc tatcggctaa attggctgtg gtattggcac
     5881 cagatgccgt aacggtaaat gccgaattag ccgtaatggc gccatcaaag gtggcacttt
     5941 tgccacccgc catagaaaga ccaagctgcc cggatgctat attaagtgcg ctgagtgtta
     6001 tgtcctgggt aacattaatg gaagcactac ccgccgaagt ggcggtagtt tccagcgaag
     6061 ttacagtgcc aagtttattg ccgttgttaa tcagtaatac tgatgtgccc ttaaatgcgg
     6121 ctgtatttgc atcgatactg gtgccgccaa attggcgaat atctttagtg gtagaaagtg
     6181 ataacccttg ggcgctgaca ttgcccagcg taagatacga attatcgcct ttaatggtag
     6241 aactaccggc gttagtaatg gctatggttt tgcctgcagc taccttggca ttgttaacca
     6301 aggtatcgat attattgtca tttgagttgg tgccagcatt ggcgccagac gtgccaaaaa
     6361 tattcccggc ttcaccaagc gtaatatttc cgccgttggt tttaagtgtt gtagtgccat
     6421 gaacgcttaa agctttaccc gccgcctgtg taatactgat gcttttatcc gagctgcgat
     6481 tttgaatggt taaatcgccc gcgcatgtac ccagcgtaca gaaatggtca gttgttatag
     6541 agcctaaaac aatattgcta tcgctggata aaataacgtt gccaccgtcg gagcgggtgg
     6601 cgttgccata cccgccattg ccggttaccg cattgtccgg cgagctgttc gcacgatcgg
     6661 tgtcaataag tgcacttgta ccaaactgta taccggcgcc agtaaattcc acattaccgc
     6721 cagaggtata aacggtatta cccacaatta ccgaacccgt accatcctta ccgccagtca
     6781 tcacctccgc atcggcattg gcaacgatat tcaacccgtc ccggccgtca ccatagctat
     6841 caaatatatt acctttgaaa ttaatatcac ctgctgcatt taacgtgaaa atggtttttt
     6901 tatctgcttc aggagtgtta ttgggtaggg tccagttttc attacttgaa ttagcgccgt
     6961 tataggcggc gaaattatta aaatccagat ttttttcaaa actaatagaa ccctggctag
     7021 tgacggttaa agagccagct tttgtggccg tactattaaa agtgcgcgat aaatccaaca
     7081 ctccaagtga gacattacct tttgcatcga tggttatatc accagcgccg ttcgtggcgg
     7141 aggtgttcag tgttgcatta gccgccgata cggtgccacc aataggaact tgggaactgc
     7201 ctattttgat atcgccgccg ttagt
//
LOCUS       DAOQGS010000167         7223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5046_length_7223_cov_4.668806, whole genome shotgun sequence.
ACCESSION   DAOQGS010000167 DAOQGS010000000
VERSION     DAOQGS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5046_length_7223_cov_4.668806"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKZ52_07970"
     CDS             complement(<1..225)
                     /locus_tag="PKZ52_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNG59747.1"
                     /translation="MNKNRRQIVIGNWKLNGSISSVTDLLHELVAGWVGVHQAEVVVC
                     PAHVHLLQAYNELTKSFFSNRWISSLLPHTW"
     gene            complement(411..1751)
                     /gene="glmM"
                     /locus_tag="PKZ52_07975"
     CDS             complement(411..1751)
                     /gene="glmM"
                     /locus_tag="PKZ52_07975"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNG59748.1"
                     /translation="MARKYFGTDGIRGRVGEAPITPDFFLKLGWAVGRVLASDPKGNP
                     GRVLIGKDTRISGYMFEAALEAGLISAGVDVGLLGPMPTPAIAYLTRTYKAAAGIVIS
                     ASHNPYTDNGIKLFSTDGTKLSDELELAIEAQLDLPMQIAERLGKAERLEDVRGRYIE
                     FCKGTLPWGFNLKGLNIVLDCANGATYRVAPAVFEELGAKVKAIAKDPDGVNINLNCG
                     STKMKLLQQQVLERGADLGIAFDGDGDRVMFVDSTGDVIDGDQLLFIIAAYQHKHGGG
                     CPGVVGTLMSNFGFEVALFQLGIPLLRANVGDRYVIEGMKDKGWNLGGENSGHLVCSD
                     VTTTGDGIIAALQVLRALQADGRPLHLAKLAMQKMPQVMINVPMASKQNPMANPSVQA
                     AVAAVEHKLGNDGRVLLRPSGTEPVVRVMVEGKQPDTVERLCHELAEQVKEALA"
     gene            complement(1766..2602)
                     /gene="folP"
                     /locus_tag="PKZ52_07980"
     CDS             complement(1766..2602)
                     /gene="folP"
                     /locus_tag="PKZ52_07980"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNG59749.1"
                     /translation="MKLICGSRELDLTSPKIMGILNTTPDSFSDGGALYAEGNLDTAA
                     ALKKAERMLSEGAALIDVGGESTRPGAAAVSLQEELDRVVGMVEIIAARLDVIISVDT
                     SKPEVMRAAAAAGAGFINDVRALQWPGALEAASQLDLPICLMHKQGEPEDMQHNPSYR
                     HVDREVRDFFYDSIQRCRGYGISAQRLVLDPGFGFGKTDEHNLELIKALPQFAEIGCP
                     LLVGLSRKSMIGRLLGRELSQRLPGSLGFGLVALQRGAKILRVHDVAASRDIVRVYEL
                     TY"
     gene            complement(2738..4636)
                     /gene="ftsH"
                     /locus_tag="PKZ52_07985"
     CDS             complement(2738..4636)
                     /gene="ftsH"
                     /locus_tag="PKZ52_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNG59750.1"
                     /translation="MAKNLVLWLVIAAVLFSVFQNFGGRDNKDELTYSQFVQEVQTDR
                     IAEVRVDGQMIIARRTDNSTYTVVRPDLTDDKLMDDLLAHKVTVKGTEPHRASLWEQL
                     LVASFPILLFIGVFVFFMRQMQGGAGGRGGPMSFAKSKARLLGEDQVKTTFADVAGVD
                     EAKEEVSELVEYLRDPSRFQRLGGRIPRGVLMSGPPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGASRVRDMFDQAKKQAPCIIFIDEIDAVGRHRGGGHGGGNDEREQT
                     LNQLLVEMDGFEGSEGIIIIAATNRPDVLDKALLRPGRFDRQVYVGLPDIRGREQILK
                     VHMRKVPIDDKVEPSIIARGTPGFSGADLANLVNEAALVAARANKRLVGVEEFERARD
                     KIMMGAERRTMVMSEKEKINTAYHEAGHAIVGRLVPEHDPVHKVSIIPRGRALGVTMY
                     LPTEDKYSESKRGLESMLCSLFGGRIAEELVLGFDGVTTGASNDIERATNLAHRMVTK
                     WGLSEKLGPLHYGETESGIPGIGTPHYSGATSKLIDEETRRIIDECYGRAKRILEENR
                     DILEAMKDALMEYETIDSEQVDDLMARRKVRPPRDWSDGDFGGRKPRSEAAEEREAGL
                     VSGQTKEV"
     gene            complement(4744..5373)
                     /gene="rlmE"
                     /locus_tag="PKZ52_07990"
     CDS             complement(4744..5373)
                     /gene="rlmE"
                     /locus_tag="PKZ52_07990"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNG59751.1"
                     /translation="MARSKSSSRWLQEHFSDRYVKESQKDGYRSRASYKLLEIQEKDR
                     LIKPGMTVVDLGAAPGGWSQVAAQLVGIKGSVLATDILLMDNIAGVDFIQGDFTEESV
                     FNTLLERLGEHKADLVMSDMAPNMSGIAGVDQPKAMYLCELALDMARHTLKPGGDFVC
                     KVFQGEGIDEWMKLCRQEFAKCQVRKPDASRARSKEVYLVAKQFKARSS"
     gene            5484..5783
                     /gene="yhbY"
                     /locus_tag="PKZ52_07995"
     CDS             5484..5783
                     /gene="yhbY"
                     /locus_tag="PKZ52_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNG59752.1"
                     /translation="MTLSTETKKHYRTIGHGLNPIVTIAGNGLSDGVVAELERALNDH
                     ELIKVKLAINDREERKELVVALCETTGAHLVQEIGKVALIYRAAARPDPKKTNIR"
     gene            5985..6800
                     /locus_tag="PKZ52_08000"
     CDS             5985..6800
                     /locus_tag="PKZ52_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59753.1"
                     /translation="MVVLKTSASLKTPVLIIGAWFFACAVCAEEVSKIEDNSFLIEEA
                     YNQEEGVIQYIQAYQYSHKTKEWLYTFTQEIPFPNQTHQVSYTLPVAHVPGDDATTGL
                     GDIGLNYRYQLIANETVAFAPRFSLILPTGDYKDGLGNGVLGYQVNLPLSIALSDQWI
                     SHWNLGGTYTPNAKEASGAKADINAFSYGASLIYLQTETFNWMLEYVRTNSQVVMPDS
                     SKERDDVAFINPGFRLAKNYASGWQMVSGLSFPIGVGPSRDDNGVLLYLSFEK"
     gene            6860..>7223
                     /locus_tag="PKZ52_08005"
     CDS             6860..>7223
                     /locus_tag="PKZ52_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59754.1"
                     /translation="MKKPLFHTLCIYAALVLWLFASVSGMHGRHCLDGLEPPVSVHFD
                     LLDSREDSHEESQAGHQDVENKAQVSALKLGNMDTLFFAALLWIACVWPLVRGQSYFL
                     FKTPRAWLDTTGLRPPLRP"
BASE COUNT         1551 a         1950 c         1980 g         1742 t
ORIGIN      
        1 ccacgtatgg ggaagcagcg aactgatcca cctgttgctg aagaatgatt tggtcagctc
       61 gttataggcc tgcagcaggt gcacatgggc ggggcaaaca actacttcag cctggtgaac
      121 accaacccaa ccggcaacca attcgtgcaa aaggtcagta actgaactga tgctgccatt
      181 gagcttccag ttgccgatca ctatctggcg tcgattttta ttcacgtagg ttcagttcgc
      241 tgttgtctct actgcccgcc tttcgacgac cctcgctgga gcgggcggca atcttagcta
      301 agaagcccca ccatagcaat gctgcggagg tgtaatttga aaatttcttg cgcgctcaac
      361 taggtggttt ccatcgcgat aaacactggc tcaaagcggc cacagtctta tcacgctagg
      421 gcttctttca cctgttcggc taattcatgg cacagtcgct ccaccgtatc cggctgtttg
      481 ccttccacca taacgcgcac caccggctcc gtgcctgaag ggcgcagcaa gactcggccg
      541 tcattaccga gtttatgctc taccgctgca acggccgctt gtacgctggg gttggccatg
      601 ggattttgtt tgctggccat gggtacgtta atcatcacct gtggcatttt ctgcatggcg
      661 agtttagcta ggtgcaaggg gcggccatcg gcttgcaggg cacgcaggac ttggagggcg
      721 gcaataatgc catcgccggt ggtggtgacg tcgctgcaga cgagatggcc ggagttttca
      781 cctcccaggt tccacccctt gtccttcata ccttcaatta catagcgatc gcccacattg
      841 gcgcgcagca gaggaatgcc cagttggaaa agcgcgacct caaagccaaa attgctcatc
      901 aatgtgccga caacaccggg gcagccgccg ccgtgtttgt gttggtaagc ggcgatgatg
      961 aacagcagtt ggtcgccgtc gataacatcg ccggtactgt ctacaaacat gacgcggtcg
     1021 ccatcaccgt caaaggcgat gcctaagtcg gcgccgcgtt caagcacctg ctgttgcagg
     1081 agtttcattt tcgtagagcc gcaattgaga ttgatattga ccccatctgg gtctttggca
     1141 atggctttaa ccttggcgcc cagctcttca aatacggctg gagcgacgcg ataggtggcg
     1201 ccgttggcac agtccaacac tatgttaagg cccttcaaat taaagcccca gggtagggtg
     1261 cctttgcaga attcaatata gcggccgcgc acatcctcta ggcgctcggc tttgcctagg
     1321 cgctcggcaa tctgcatggg taaatctagc tgcgcttcaa tggccagttc taattcgtcg
     1381 gagagtttgg tgccgtcggt gctgaataac ttgataccgt tatcggtgta ggggttgtgg
     1441 gaggcgctga tgacgatgcc tgcggcggct ttataagtgc gagtgaggta ggcgatggcg
     1501 ggggttggca tggggccgag taatcctacg tccacaccgg cgctgatcag tcccgcctct
     1561 aaagcggctt caaacatata gcctgagatg cgggtgtctt tgccgattaa cactcggccg
     1621 ggattaccct tagggtctga cgccagtacg cggccgacgg cccagccaag ttttaagaag
     1681 aaatccggtg taattggggc ttcgcccact cgtccgcgta tgccatcggt gccaaagtac
     1741 ttacgtgcca cgtgttatct ccaaatcaat aggttaattc atacactcta acaatatctc
     1801 gactggccgc cacatcgtga acacgcaata tttttgcgcc gcgttgcagg gccacgaggc
     1861 caaagcctag gctaccgggc agccgttggg atagctcgcg cccgagtagc cgcccgatca
     1921 tggatttgcg ggacaaccct accagtagcg ggcagccgat ttcggcaaac tgcggcaagg
     1981 ctttgataag ttcaagattg tgctcgtcgg ttttgccaaa cccaaagccg gggtcaagca
     2041 ccaagcgctg ggcggaaata ccatagccgc ggcagcgctg aatactatcg tagaagaagt
     2101 cccgcacctc gcgatccaca tggcgataac ttgggttatg ttgcatatcc tccggctcgc
     2161 cctgtttgtg catcaggcag atgggtaaat ccaattggct tgccgcctct agggcgccgg
     2221 gccattgtag ggcgcgcaca tcattaataa acccggcgcc tgcggcggca gcggcgcgca
     2281 tgacttcagg cttgctagtg tccacggaaa taataacgtc taagcgtgcc gcgattattt
     2341 caaccatgcc cacgacccgg tctagctcct cttgcaggct cactgctgcg gcgccggggc
     2401 gggtggattc cccgcctaca tcaatcaggg cggcgccttc gctgagcatt cgttcggctt
     2461 ttttaagcgc tgcagcggtg tcgagattac cttcggcata gagggcgccg ccgtcggaaa
     2521 aggaatcggg ggtggtgttg agtatgccca ttattttggg gctggttagg tccagctcgc
     2581 ggctgccgca gatgagtttc acaatgacgg ccctgggtgg gtttgaattg cacaattcat
     2641 gggggtatca cgccttgtaa cggcagtaaa taaagattgg aattttggcg gcaataaaaa
     2701 agccggtatt accggctttg cgtggggcga gaggggttta cacctctttg gtttggccgc
     2761 tgaccagccc agcttcgcgc tcttccgccg cctcggaacg gggcttgcgg ccaccgaaat
     2821 caccgtcgga ccagtcccgt ggcgggcgca ctttgcggcg cgccatcagg tcatccacct
     2881 gctctgaatc gatggtctca tattccatca gggcgtcttt catggcttca agaatatcgc
     2941 ggttttcttc taggatgcgt ttagcgcggc cataacactc atcgatgatg cggcgggttt
     3001 cctcgtcgat cagctttgag gtggcgccgg aatagtgggg ggtgcctatg ccggggatac
     3061 cggattcggt ctcgccgtaa tgcagcggcc ccagtttttc cgacagcccc cacttggtca
     3121 ccatgcggtg ggcaaggtta gtggctcgtt caatatcgtt ggaggcgcct gtggtaacgc
     3181 catcaaaacc cagtaccagc tcttcagcta tgcggccacc aaacaaggag cagagcatgg
     3241 attcaaggcc gcgtttactt tcactgtatt tatcttcagt gggcaaatac atggtgacgc
     3301 cgagggcgcg gccgcgcgga ataatgctca ccttgtgcac cgggtcgtgc tcgggcacca
     3361 ggcggccgac aatggcgtgg ccggcttcgt ggtaggcggt gttaattttt tccttttcgc
     3421 tcatcaccat ggtacggcgc tccgcgccca tcatgatttt atcccgcgcg cgctcaaatt
     3481 cttccacacc cactaggcgc ttattggctc gggcggccac cagggcggcc tcattgacta
     3541 gattggcaag atcagcgccg gaaaacccgg gtgtgccacg ggcgataatg ctgggctcca
     3601 ccttgtcgtc gatgggcact ttgcgcatgt gcacctttag aatttgctcg cggccacgaa
     3661 tatcgggcag gcctacatac acttggcggt caaaacggcc ggggcgcagc agggctttgt
     3721 ccagtacgtc cgggcggttg gtggcggcga tgatgatgat gccttcactg ccttcaaagc
     3781 cgtccatttc tactagcaat tggttgaggg tctgctcgcg ctcgtcgttg ccgccgccgt
     3841 gcccgccgcc acgatggcgg ccaacagcgt caatttcgtc aataaagata atacagggag
     3901 cttgtttttt ggcttgatca aacatgtcgc gcacgcggga ggcacccacg cccacaaaca
     3961 tttccacgaa atcagaaccg gagattgaaa agaaaggtac tttggcttcg cccgcaatgg
     4021 cctttgcgag gagggtttta ccggtaccgg gcggcccgga cattaaaacc ccgcgcggga
     4081 tgcggccacc taagcgctgg aagcgagagg ggtcgcgcag gtattccact aattcggaga
     4141 cttcttcttt ggcttcatca acacctgcca cgtcggcgaa ggtggtttta acctgatctt
     4201 cacccagcag gcgagccttg cttttagcaa aactcatggg gccgccgcgg ccaccggcac
     4261 cgccttgcat ttggcgcata aaaaacacaa atacaccgat aaacagcagg atggggaagc
     4321 tggcgacgag caattgctcc cagaggctgg cgcggtgggg ctcagtgccc ttcacggtga
     4381 ctttgtgggc cagcagatca tccatcaatt tgtcgtcagt gagatcaggg cgcaccacgg
     4441 tataggtgga gttatccgtg cggcgggcga tgatcatctg cccatccacg cgcacctcgg
     4501 cgatacgatc cgtctgcact tcctgcacaa attgtgagta ggtcagctca tctttgttgt
     4561 cacgcccgcc aaaattttgg aagacggaaa acaaaacggc ggcaattacc aaccagagca
     4621 ctagattttt tgccatgtcg ttcaagagga tctcctcgga tatcctaaga tcaaggatgt
     4681 gtactaagtg gagtatagcg ctagcgggct agacgcctat agcgtaacta cgtttttctt
     4741 accttaactg gaccgtgctt taaattgttt ggcgactaaa taaacttctt tggagcgcgc
     4801 gcgggaggcg tcgggcttgc ggacttggca tttggcaaat tcctgccgac acagcttcat
     4861 ccattcatcg attccctcgc cttgaaatac tttacagaca aaatcaccgc cgggcttgag
     4921 cgtgtggcgg gccatgtcca atgccaattc acacaggtac atggctttgg gctgatcaac
     4981 acccgcaata ccactcatat tgggggccat atccgacatt acaagatcgg ccttatgttc
     5041 acccaagcgt tcaagcaggg tgttaaacac agactcttcg gtgaaatccc cttggataaa
     5101 gtcaacacca gcaatgttat ccattaatag aatgtcggta gctagcaccg agccttttat
     5161 acccaccagc tgagcggcca cctgggacca gccgccgggg gctgccccca gatccactac
     5221 tgtcataccg ggtttaatca ggcggtcctt ctcttggatt tccaataatt tgtaactagc
     5281 gcgggagcgg tagccgtcct tttgagattc ttttacatag cggtcgctga agtgttcttg
     5341 tagccagcga ctgctacttt tggaacgggc cataaaaaac gcaagcctca gggggtgtgg
     5401 agtgtttaca atagcggcat attacggaac ctggctcatt ctgagaagtt ccgtgttgtg
     5461 ttcgctcgta cgtagatccc accatgaccc taagcaccga aaccaaaaaa cactaccgca
     5521 ccattggcca cggcctgaac cctatagtaa ctatcgctgg caacggcctc tccgatggcg
     5581 tagtggcgga actcgagcgc gccttaaacg accatgagct aattaaggta aaacttgcca
     5641 ttaacgatcg cgaggagcgc aaagagttgg ttgtggcttt gtgtgagact accggggcgc
     5701 atttggtgca ggaaattggc aaagtggcat tgatttaccg cgccgccgcc cggccagatc
     5761 ctaagaaaac caatatccgc taaatctagt tacccgtccg ccttctttat tgtttcatta
     5821 attttcctgc tgcacttttg cctggtatgc gccctttggt gtgactgcgg cggcgctggg
     5881 tttgcggtat taacatcatc tgttaaatgc ctgctcccgg cgcaagcaaa ttccctgtgc
     5941 taggctttag ccattgctta ggcctatcac agatggaatt ttttatggtt gttttgaaaa
     6001 cttcagcttc gttaaaaacg cctgtattaa ttattggcgc ttggtttttt gcctgtgctg
     6061 tttgcgctga agaggtgagt aagatcgagg ataactcgtt tttgattgag gaggcataca
     6121 atcaggaaga gggagttatt caatatattc aggcctatca gtactcccac aaaaccaagg
     6181 aatggctgta cacatttacc caggaaattc ctttccccaa tcaaactcac caggtctcct
     6241 acaccctgcc ggtggcccat gtgcccggcg atgacgccac cactgggttg ggtgatattg
     6301 gccttaatta ccgctaccag cttattgcca acgagacggt cgcatttgcg ccgcgttttt
     6361 cgttaatcct gcctaccggt gattacaaag atggtttggg taatggcgtg ctggggtacc
     6421 aggtgaattt accgctgagt attgccctgt cggatcaatg gattagccac tggaatttag
     6481 gcggtactta tacccctaac gccaaagagg cttcaggggc caaggcggat attaatgcct
     6541 tcagttacgg cgccagcctt atttatttgc agacagaaac ctttaactgg atgctcgaat
     6601 atgtgcgcac caactcccaa gtggtaatgc ccgacagctc caaagagcgg gatgacgtgg
     6661 cgtttattaa ccccggattt cggctggcaa aaaattatgc ctccggttgg cagatggtgt
     6721 cgggcctgag ttttcctatc ggcgttggcc catctcgcga tgataacggt gttttgcttt
     6781 acctctcgtt tgagaaatag tttgcgcgaa tggctgcgca aagcccctat gatggctggc
     6841 cgatgattgg gtcaacactg tgaaaaaacc gcttttccat acactgtgca tttatgctgc
     6901 ccttgtgctc tggctgtttg ccagtgtcag cggcatgcat ggccgccact gtttggacgg
     6961 cttggagccg ccagtgtcgg tgcattttga cctattggat agccgcgagg acagccacga
     7021 ggagagccaa gcgggccatc aggatgtgga gaataaggcg caggtaagcg cacttaaact
     7081 tggcaatatg gatacgcttt tcttcgccgc gcttttatgg atcgcctgtg tctggcctct
     7141 ggttcgcggc caatcttatt tcctctttaa aacgcccaga gcctggctcg ataccaccgg
     7201 cctgcgaccg ccgctgcgcc ccc
//
LOCUS       DAOQGS010000168         7197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5067_length_7197_cov_6.882666, whole genome shotgun sequence.
ACCESSION   DAOQGS010000168 DAOQGS010000000
VERSION     DAOQGS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5067_length_7197_cov_6.882666"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKZ52_08010"
     CDS             complement(<1..264)
                     /locus_tag="PKZ52_08010"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNG59755.1"
                     /translation="MRQALELARQAGVAGEVPVGAVLVWQGEVIGRGANCPIGQCDPS
                     AHAEIQALRAAGRATGNYRLVDSVLYVTIEPCAMCWGALVHARV"
     gene            complement(275..535)
                     /locus_tag="PKZ52_08015"
     CDS             complement(275..535)
                     /locus_tag="PKZ52_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNG59756.1"
                     /translation="MNQVDLPLGYYRHFKGAIYRVAGLARDCESQQWMVYYQCCYGDW
                     SYWLRPLDNFCQTVLKEGIELPRFAFLGERLPVELAGAQGGV"
     gene            715..2634
                     /locus_tag="PKZ52_08020"
     CDS             715..2634
                     /locus_tag="PKZ52_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNG59757.1"
                     /translation="MLDQSSLAQLSQLKTAIIASKEYAEGTVVGSKGNYGFVRLDDGR
                     DAFLNNEKMQRVLPGDRVKVLLTKNEKDQLEGQLEALLSTGLKRFVAEYKTKDHLHFV
                     LPQIEHFSRWIFVPPQFRGKCQAGDYVLCELTSHPYGDGKAAAKVVARLGQPTDPYIE
                     HKVTIAKHGLYRYWHKDALAQMEACANDAAALNGREDFTSIPFVTIDGATTQDMDDAV
                     YCEPQADGFLLQVAIADPASFIPPNSPIARAARDNAQTVYLPGETLSMLPERLSTYSF
                     SLVEQQVRPALIVTIELASDGRVRNYQFKQGKIQSRHKLTYSQVAAFIEGKEPLAIDP
                     ACQTSLQTLSSLAQVRLALRQAEHWIPDDHQDFSFLLNPQGKIERIERRERTCAHRMI
                     EEAMLLANGCAGEFLAAHKAGLHAVHGGFKAERLGEIRALLKEELGELPPGDWSNLPD
                     YLQLLKTLQNSPKADIVLPPLKRMMRASELSLEACAHMGLGFNHYATITSPIRRYADL
                     YNHWAITQILNQQTPFPINPKLLEQVQETLSRGRQAVRELELNLSCQYLADHIGLEDQ
                     AFIRIITQQGFGVKLQATGLEGFVNFDKSQAKTFDAKRMTLSLGERCFRLDDTVRVQV
                     VEVDRAKNRVKMQLV"
     gene            3015..4079
                     /locus_tag="PKZ52_08025"
     CDS             3015..4079
                     /locus_tag="PKZ52_08025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNG59758.1"
                     /translation="MQPIPPVKHQYLLLDALRGLAALFVVIWHTSLYWSPLPFQHTYL
                     AVDIFFVLSGFVIASAYKEKLRSRTLSIRGFLVTRLVRLYPMYFFGFLLGAIAFMLGA
                     FDNSQHPLLEFFCAILMLPAGITAAGHTLFLFNLPSWSLFYELVANLFVALIYRFGHT
                     QLLPAVVLVLGAGLTAITIYKGGIDIGPFGGYKNVIIASIRSVFGIFMGLWLNHIHRQ
                     HTIRIPVVWGWLALVILVLLLSLPSLQPWDVWVDVAATVFIIPTCVFLLASIRPNLPR
                     ATQTIATFLGDTSYPLYAIHYPLMLIGLAVWPETIKHFKNYSGLILLVLLFALSFGLF
                     KWFETPARRWLSAQTKGLRR"
     gene            complement(4361..4882)
                     /locus_tag="PKZ52_08030"
     CDS             complement(4361..4882)
                     /locus_tag="PKZ52_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNG59759.1"
                     /translation="MVNKLDGESNALRSVLLLFNIQKAGNFGKLIRTANALGVAQVCI
                     VGRRDFATHGHHHTKIATEFSHFYKPKDALEHYRKQGFDLVAVEIGAQAVNINRHQFV
                     RDTVFVMGNETTGVSPELLALCNYAVYIPQYGAGASLNVNVAAGIVLNAFNAQRPSHN
                     PIAGAKFCPDELC"
     gene            complement(4872..5465)
                     /locus_tag="PKZ52_08035"
     CDS             complement(4872..5465)
                     /locus_tag="PKZ52_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNG59760.1"
                     /translation="MSDVAAIIDFLIEVEKLKGVERKTSPIGLARKENSAEHSWHLCV
                     LAVLLHGKANAAVDLLKVLKMLIVHDLGEIDAGDTLIYAAAENTQAREEAGMRRICQL
                     LPPPLGDELMALWLEFVGGATAEAQFAQAIDRVPPIVQNLQTGGQTWRPLGIAREQVL
                     AKNSLIKLGCTPLWEYLLPRINAYFTPNAARENADGE"
     gene            complement(5462..7192)
                     /gene="recJ"
                     /locus_tag="PKZ52_08040"
     CDS             complement(5462..7192)
                     /gene="recJ"
                     /locus_tag="PKZ52_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNG59761.1"
                     /translation="MASAKTIVRRVVPPLPAVNDHPILNRILLARGVTAAAELDYGLA
                     QLIDPRQLKGIEQAIDLLTQAYVAQKRILIVGDFDADGATSTALAVRGLRALGFKQVD
                     FLVPNRFEYGYGLTPEIVAVAQQYQPELLITVDNGISSVAGVEAANALGMQVLITDHH
                     LPGDALPPAAAIVNPNQPGCDFPCKALAGVGVMFYVLIALRSHWRSCGLFAERSEPNL
                     AEFLDLVALGTVADVVPLERNNRLLVAQGLKRLRAGYCCAGIKALLQVAGRSSDKITA
                     MDLGFIIGPRLNAAGRLDDMSIGIQCLLTDNPAQALEYAQELDALNRERRSIEAGMQK
                     ESQVLLAQLESELNGQLPQGLCLYHPQWHQGVIGILASRIKDKLHRPVIIFAENAPGE
                     LKGSGRSIAGLHLRDALDEIAKRAPYLLKKFGGHAMAAGLSLEERYLPEFKHLFNEVV
                     TAHIGPEGVAAAIISDGDLLGEDFSLDLADQLAASGPWGQAFAEPTFDGEFIVHQQRL
                     LNDKHLKMTVSPVQSPEVLLDAIAFNVDAQPWSQHLQAKMRLAFKLTPNEFRGQRSVQ
                     LLVDYGEPVL"
BASE COUNT         1761 a         2101 c         1696 g         1639 t
ORIGIN      
        1 cacacgggcg tgcaccaggg caccccaaca catggcacag ggttcaatgg ttacatacaa
       61 cacgctatcc actaatcggt aattgccagt ggcccggccc gccgcccgca gggcctgaat
      121 ctccgcatgg gcactggggt cgcactggcc gatagggcaa ttggcgcccc ggccaatcac
      181 ctcgccctgc cacactagca ctgcgcccac cggcacttct ccggcgacac cagcttgcct
      241 cgccaactcc agcgcctgcc gcatataaaa gaagtcagac tccaccctgc gcccccgcca
      301 attccacagg taaccgctcc cctaaaaacg caaaccgcgg cagctcaatc ccttctttta
      361 gtacagtctg gcaaaagtta tccaaaggcc gcagccaata actccaatcg ccgtaacaac
      421 actggtagta caccatccac tgttgggatt cgcagtcccg cgccaggcct gcaacacggt
      481 aaatggcacc cttaaaatgg cggtaatagc ccaggggcaa atcaacttgg ttcataaaac
      541 gctgtgccat ggttgagaaa agcggccagt gtgcgacagg ctacaaaggc aggtaaagct
      601 gtttggcaaa acggcaaaag cttggcgcca ggcccgtggc ggcggggtta aatttgccgc
      661 agccctaggg gttcctgtta tcatgcccgc ccaaaaaatt attcgagatc caccatgtta
      721 gaccagtcca gccttgcgca gctctcgcaa ctcaagaccg cgattatcgc cagtaaagaa
      781 tacgcagagg gcacagtggt gggctccaaa ggtaattatg ggtttgtgcg tttagacgac
      841 ggccgcgatg ccttcttgaa taatgaaaaa atgcagcggg tgctgccggg ggaccgggtt
      901 aaggtactac tcaccaaaaa cgaaaaagat cagctggaag gccaattgga ggcactgctc
      961 tccaccggcc ttaagcgttt cgtggcggaa tacaaaacca aagaccacct gcactttgta
     1021 ctgccacaaa tcgagcattt ctcccgctgg atctttgtgc cgccgcagtt tcgcggcaaa
     1081 tgccaggccg gtgattatgt gctctgcgaa ctcaccagcc acccttacgg cgacggcaag
     1141 gcggcggcca aagtggtggc ccggttaggc cagcccaccg acccctatat cgaacacaag
     1201 gtcactatcg ccaaacacgg cctgtaccgc tactggcaca aagacgccct cgcccaaatg
     1261 gaggcctgcg ccaatgacgc cgccgcccta aacggccggg aagatttcac ctcgattccc
     1321 tttgtcacca tcgacggcgc caccacccag gatatggacg acgcggttta ttgcgagccc
     1381 caagccgacg gttttttact gcaagtggcc atcgccgacc cggccagttt tattcccccc
     1441 aacagcccta tcgcacgggc ggcgcgggat aacgcacaaa ccgtgtatct gcccggcgaa
     1501 accctctcca tgttgccaga gcgtctctcc acctattcct tctccttggt agagcaacag
     1561 gttcgccccg cgctgatcgt caccatcgag ctggcatccg acggccgggt gcgcaattac
     1621 caatttaaac agggcaaaat tcagtcccgc cacaagctca cctacagcca agtggccgct
     1681 tttattgaag gcaaagagcc cctggctatc gatcccgcct gccaaaccag cttgcaaacc
     1741 ctctccagcc tggcgcaggt gcgcctggcc ctgcgccagg cggaacactg gatccccgat
     1801 gatcaccaag actttagctt tctgctcaac ccacagggga agatagagcg cattgagcgg
     1861 cgcgagcgca cctgtgccca ccgcatgatt gaagaggcca tgttgctggc caacggctgc
     1921 gccggtgaat ttttggccgc ccataaagcg ggcctacatg cggtgcacgg cggctttaag
     1981 gcggagcgct taggggagat tagagcgctg ctcaaagaag agctcggcga gttaccccct
     2041 ggggattggt cgaacctacc tgattattta caactgctca aaacgctgca aaacagcccc
     2101 aaggcagaca tagtgttgcc tccgctgaaa cgcatgatgc gcgccagcga attaagcctt
     2161 gaggcctgcg cccatatggg cctcggcttt aaccactacg ccaccattac ctcccccatt
     2221 cgccgctacg ccgatctcta caaccactgg gccatcaccc aaatactcaa ccaacaaacg
     2281 cccttcccca tcaaccctaa attgctggaa caggtgcagg aaaccctcag ccggggccgc
     2341 caagcggtgc gcgagctgga attgaacctc tcctgccagt acctggccga ccacataggc
     2401 cttgaggatc aggcgtttat ccgcatcatc acccagcagg gctttggtgt taagttgcaa
     2461 gcgaccggcc ttgaaggctt tgtgaacttc gataaaagcc aggcaaaaac ctttgacgct
     2521 aagcgcatga ccctaagcct gggggagcgc tgcttccgcc tggacgacac agtgcgggtg
     2581 caggtggtgg aagtagaccg cgcgaaaaat cgcgtgaaaa tgcagttggt ttaaattgct
     2641 gggcgctcgg ttttggtcga gcgcccaatt cacagtgcac ggttaaaatg aaggttccaa
     2701 tttaagcctg cgaattaggc cgagccaaat aaaattccaa aaccctgcgg atacagaggc
     2761 acctgcacga attcaatttt cctgatggtc cctaggcatt tttgtgctgg cggcacggat
     2821 gcaggagcca ttgccgaagc agggcgcgta tagggtataa atgaccgacc gagcacacaa
     2881 acaacaccgg gtgaaagaca acacaaagcg aaaagtgaat ttccggaagg tgtccataac
     2941 aataaaaaca acaggtgtag gcattcacaa ctagtctttt gctaagcact caatccgtac
     3001 atccataaat cgccatgcaa cccataccac cggttaaaca tcaataccta ttacttgatg
     3061 cccttcgcgg gctcgccgca ttatttgtgg ttatttggca cacctcgctt tattggagcc
     3121 cgctgccgtt tcagcacacc tatttggccg tcgatatttt tttcgtactc agcggctttg
     3181 ttatagcatc cgcgtacaaa gaaaaactgc gcagccgcac actgagtatt cgcgggtttt
     3241 tagtcacccg cctggtgcgc ctttatccca tgtatttttt tggcttttta ctgggcgcaa
     3301 tcgctttcat gctcggtgcg tttgataatt cccaacatcc actgctggaa tttttttgcg
     3361 ctattttaat gctgcccgca ggcataaccg ccgcgggtca tacacttttt ttatttaatt
     3421 tgccttcctg gtcgctcttt tacgaattgg tagccaatct atttgtggcg cttatctatc
     3481 ggttcggcca cacacaatta ttaccggcgg ttgtattagt tttaggtgca gggcttaccg
     3541 ccattactat ttataaaggt ggcattgata taggcccgtt tggcggttac aaaaacgtca
     3601 ttatcgccag tatacgttcc gtgtttggca tttttatggg cctgtggcta aaccatattc
     3661 accgccaaca cacaatacgt ataccggttg tttggggctg gctggcgctc gttattttgg
     3721 tgctgctgct gtcattgcca tcactgcagc cctgggatgt gtgggttgat gtggcggcca
     3781 cggtgtttat tattcccacc tgtgtttttc tcctggccag catccgcccc aacctgcccc
     3841 gcgcaacgca aacaatcgca acctttttag gcgacaccag ttaccccctc tacgccatac
     3901 attatccgct gatgttaata gggcttgcag tttggccgga gacgataaaa cactttaaaa
     3961 attacagtgg attaatttta ttagtcctgc tgtttgctct ctcctttggt ctttttaagt
     4021 ggtttgaaac cccggcacgg cgctggctaa gcgcccaaac caaagggttg cggcgttaag
     4081 gaacctctga aaaattacct cactcgccct agccatgttg aattcggcaa aaaaatgctc
     4141 atttactgcg tgtaaactcg gcaattgctc ctgcattgct ctacctcctg cctccctgca
     4201 gtcgtcggca attgctcctg cattgctcta cctcgatttc attcccgatg aaattctacc
     4261 tcccccttcc atggggtcgt cctgcctccc tgcagtcgtc cgcttttttt gccaaattcg
     4321 cctagctagt gcttcgccgc ctacattttt cagaggttcc ttaacacaat tcatcggggc
     4381 aaaacttggc cccggcaatg ggattgtggc tgggccgttg ggcattaaat gcatttaaca
     4441 ctatgcccgc cgccacattt acatttaagg aggcgccagc gccatactgg ggaatataaa
     4501 cggcgtaatt acacaaggcc aataattccg ggctcacgcc agtggtttca tttcccatta
     4561 caaataccgt atcgcgtaca aactggtggc gattaatatt caccgcctgt gcgccaatct
     4621 ctacggccac caggtcaaaa ccctgcttgc gataatgctc cagggcatcc ttaggcttat
     4681 aaaaatggct aaattccgtg gctattttcg tgtgatgatg gccgtgggta gcaaaatcgc
     4741 gccgcccgac aatacacacc tgggcaaccc ccagggcatt cgccgtgcga attaatttgc
     4801 cgaaattgcc cgctttttga atattaaaca gcagcaaaac actgcgcagg gcgtttgatt
     4861 ccccatcaag cttattcacc atccgcattt tcccgtgcag cattgggcgt aaaataagcg
     4921 ttaatacgcg gcaataaata ctcccacagg ggcgtgcagc ccaatttaat caggctattt
     4981 tttgccagca cctgctcccg ggcaataccc aagggccgcc aggtttgccc gcccgtttgt
     5041 aaattctgca caatcggtgg cactcggtcg atcgcctgcg caaactgtgc ttcggccgtg
     5101 gcaccaccga caaattccaa ccacagcgcc attaattcat cgcccagcgg cggcggtaat
     5161 aattgacaga tgcggcgcat cccagcctct tctcgcgcct gtgtgttttc tgctgcggca
     5221 taaatcagcg tgtcaccggc atcgatttca cccaaatcgt gcacgatcaa catttttaat
     5281 acctttaaca aatcgacagc cgcattggct ttgccgtgca acaaaacagc taaaacacac
     5341 agatgccagc tgtgctcagc ggaattttct ttgcgagcaa ggccaatagg cgaggttttt
     5401 cgctcaacgc ctttaagttt ttccacttca attaaaaaat cgataatcgc cgccacgtca
     5461 ctcataacac cggctcccca tagtccacca gtagctgcac actgcgctgg ccgcgaaatt
     5521 cattgggggt caatttaaac gctaagcgca ttttagcttg cagatgctgg ctccacggct
     5581 gcgcatccac attaaaggca atagcgtcca gcaatacctc tggtgattgc actgggctta
     5641 cggtcatttt taaatgttta tcgtttaata aacgctgctg atgcacaata aattcgccat
     5701 caaatgtagg ctcagcaaag gcctgccccc aaggcccgct agcggcgagc tgatcggcca
     5761 gatccaagga aaaatcctcc cccagtaaat cgccgtcact gataatggca gcggccaccc
     5821 cctcagggcc tatgtgtgcg gtgaccactt cattaaatag gtgtttaaat tcgggcaaat
     5881 agcgctcttc taaacttaac cccgctgcca tggcgtggcc gccgaatttt tttaataaat
     5941 aaggcgcgcg cttggcaatt tcatccaagg catcgcgcag atgtaaaccg gcgatggagc
     6001 ggccagaacc ttttaattca cccggtgcgt tttcggcaaa aataattacc ggccgatgca
     6061 atttatcttt gatgcgggac gctaaaatac cgataacacc ctgatgccac tgggggtgat
     6121 acaaacataa accctgcggc aactgaccat ttaattcgct ctccaactgc gccaataaca
     6181 cctgcgactc cttttgcata cccgcttcaa tagacctgcg ctcgcgattt aacgcgtcta
     6241 attcctgcgc atattccaac gcctgtgcag ggttgtcggt gagcaaacat tgaataccaa
     6301 tactcatatc atccaaacgc ccagcggcat tcaaacgcgg gccaataata aaacctaaat
     6361 ccatggctgt tattttgtcg ctgctgcgcc ctgccacctg caaaagggct ttaataccgg
     6421 cacagcaata gccggcgcgc aagcgcttta gcccctgggc caccaacaag cggttattgc
     6481 gctccaaagg cacaacatcg gctaccgtac ccagggcgac caagtctaaa aattccgcca
     6541 gattaggctc actgcgctca gcaaacaaac cgcaggatcg ccagtgactg cgcagagcaa
     6601 tcaacacata aaacatcacg cccacaccgg cgagtgcttt gcagggaaaa tcacaacctg
     6661 gctggttggg attgacgatg gccgcagcgg ggggcaatgc atcgccgggc aaatggtgat
     6721 cggtaattag cacctgcatt cccaaggcat tggcagcttc tacacccgcc acactggaaa
     6781 ttccattatc aacggtaatt aataattccg gttgatactg ctgcgccaca gcgacaattt
     6841 ctggcgttaa accataaccg tattcaaagc ggttcggcac caaaaaatcc acctgcttaa
     6901 agcccaaagc gcgcaaacct cgcaccgcca aagcagtgct ggtggcacca tcagcatcaa
     6961 aatcgccgac aattaaaatc ctcttttgcg ccacataagc ctgggttaac aggtcgatgg
     7021 cctgttcaat accttttaat tgacgcggat cgatcaactg agcaagacca taatccaact
     7081 ctgccgccgc ggtgacacca cgggcaagta agatacggtt gagaatgggg tgatcattaa
     7141 cggcagggag tggcggcacc actcggcgca caattgtttt tgctgaagcc atagggt
//
LOCUS       DAOQGS010000169         7188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5076_length_7188_cov_5.036030, whole genome shotgun sequence.
ACCESSION   DAOQGS010000169 DAOQGS010000000
VERSION     DAOQGS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_7188_cov_5.036030"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1290
                     /locus_tag="PKZ52_08045"
     CDS             7..1290
                     /locus_tag="PKZ52_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNG59762.1"
                     /translation="MHNRSISVIGLGYVGLPVAVAFGRSKRTLGFDINATRISELQEG
                     QDRTLEVSDADLAVTDIIYSCNPEDLKQADFHIIAVPTPINEAKQPDLRPLISASRTL
                     APQLKKGDIIVYESTVYPGATEEDCVPVLEAGSGLVCGKDFFVGYSPERINPGDKEHT
                     FTKITKVVSGQTPEVLDIVAAVYGSVVTAGVYKASSIKVAEAAKVIENTQRDLNIALM
                     NELALIFDRLGIDTLEVLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAEKIGYHP
                     QVILAGRRINDGMGAYIAQRAIKQLILSGHSIKGARIGIFGLTFKENCPDLRNSKVID
                     VINEFASYGVELLVHDAMADSHEAEEEYGVSLVELSAMQKLDAAVFAVAHSEYAAFSA
                     AEVKASLNPGGAVFDVKAMFNPADFAAAGVAYWRL"
     gene            1555..3996
                     /locus_tag="PKZ52_08050"
     CDS             1555..3996
                     /locus_tag="PKZ52_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG59763.1"
                     /translation="MHRLKSQPLNPFNPLALAIALGASCGSVQAQDAPLIEVLVTAQK
                     RSENLQDVPISVSAVSGSKLDEVGVENIEDLTKLVPNIHLTQTGLSTQLRIRGIGSDN
                     SQGFEQSVGTYKNGIYHGRAQLLRAPIFDMERVEVMRGPQSTLFGKNSIAGAIDLITA
                     RPTDDFSGSLNTSYETQFGTREITGIVSGPLTDSLKGRLALRGYDDPGYFYNTFNHQH
                     TNQQQTTGRLSVDWQPLPELTLQYTGEKSRADSAGRNIEIVRDDPNKKGLNFATTFKL
                     LTQNNTLEGNADYKRQADAPEYSDNNVQSHSLVANYEFSGLTLTSTTGLVKYDYEENC
                     DCDFSSVNLFQLNLHEDYKQVSQEVRLTSDPGQTLDWLAGVFFQNYQQDFGDRFSVND
                     KSGLFPVAKAGGRPLPAAFGNTGIFRSFDQESDTWAAFAQVAWHFTDASQLILGGRYT
                     TESKDSHKLITMANIDGAAITDPARLASAAYVYKSVFKLDTQQTPYAPLLNAMGQPVI
                     DPATNRPRPLVPNHPGHDLQGSRDESAFTPSIKIQHDFNDDLMAYASMARGFKAGGFD
                     PRSNSNEHYEFEDEQADAYELGLKSTLLDGALEVNSALFRTDYENLQISQFDGGVGFN
                     VGNAKDTQVQGVEIDGRWAMTQHIMANYGISYLDFEYQDFKNGNCNKPQIDAGLGVDT
                     NADGKVDLCDYTGKRGVYTPKYTVNTGLTYKRELANATFTTGLDVQYVSSQQVHVNLD
                     PQGEIDAYTLVGLRIALEDERFLVALSGENLTDEYVSSYMASVPLSDSNIGSQTFYGF
                     ARRPRTYTLSMGLKF"
     gene            4759..5550
                     /locus_tag="PKZ52_08055"
     CDS             4759..5550
                     /locus_tag="PKZ52_08055"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HNG59764.1"
                     /translation="MRVPNDLGKLLVIALCSGAPWVAAESSSPEQLMGQWLNLEMQKG
                     AMQSNWNRSRDNLDQRFILLDAEQKRLKEVLSQRTVATGEVDQRRLELLQRQEKLEQE
                     QAQLDAQLKKYLQKAQLIQPRLPPPLSAHWSEKLALLGQENASASERLEHLLGLFKQF
                     DEFNQRVALHIGALDIPDANAHLQPILTSQIYLGASQGWYVSDDGKAYGYGRPTSTGW
                     KWWHGAEAAKELGRPLSAEAIARVRHILENPTTAEFISLPVKISQ"
     gene            5563..6870
                     /locus_tag="PKZ52_08060"
     CDS             5563..6870
                     /locus_tag="PKZ52_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG59765.1"
                     /translation="MNRTHAFSLRLFTLITLLLCCASLVRADVNDQLVQKISQAKNEL
                     EALEKKTSQESRSYAQRLEQKEQEVKNLRSQVASQQRVVDEQLLGVDKLKERVDQWAA
                     QSQYQKQLLIHFNQTAKLPVAVATGDELTILDQAAKQLEHALAPSWQDSKVVSAGGAI
                     IHAPVMALGPVEIAVDLQAKTAGPLVREKDREAWLLDFLPQSAQQELLTLHSTQAGEL
                     AFDPTLGNALQLRKHSDGLMVYFQKGGVWVIPIIFFGLLALLIAVLKAVQLLRLPKIA
                     HDLAEQFNQIITAHKPDARAACEALLASIGPAQRTLALIAMNNPVSQQRDDLLVAHLS
                     EHKYHLDKFMGIVATSASVAPLLGLLGTVSGMINTFKMMTIFGSGDVNTVSGGISEAL
                     ITTELGLIVAVPALVIGAVLTRQIKTYAHGMERFAIKISKIAF"
     gene            6886..>7188
                     /locus_tag="PKZ52_08065"
     CDS             6886..>7188
                     /locus_tag="PKZ52_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG59766.1"
                     /translation="MDNLSQSLALINNPIVWMTIAVAIICYGLLFRFCFCKVQDQRWF
                     ELTRDWLPGIKQMLTTLPLLGLLGTITGLKDTFIRLADGGGLVLQELITGGIGDALF"
BASE COUNT         1839 a         1794 c         1816 g         1739 t
ORIGIN      
        1 tgatttatgc ataatcgctc catctcagtt attggcttgg gctatgtggg tttgccggtg
       61 gccgtggcct tcggtagatc caagcgcacc ctcgggtttg acatcaacgc cactcgcatt
      121 agtgagttac aagaagggca ggatcgcacc ttagaagtga gcgatgccga tttagcggtc
      181 actgacatta tttactcgtg taacccggag gatttaaaac aggcggattt tcatatcatc
      241 gccgtgccca cacccatcaa cgaagccaaa cagccggatc tgcgccccct cattagcgcc
      301 tcccgcaccc tggcgcccca gctgaaaaag ggcgacatca tcgtctatga atccaccgtc
      361 tacccaggcg ctaccgaaga ggactgtgtg ccggtgttgg aggcaggctc aggcctggtg
      421 tgcggcaagg atttttttgt cggttattcc cccgagcgga tcaaccccgg cgataaagaa
      481 cacaccttca ccaaaattac caaagtggtt tccggccaga ccccagaggt gctggatata
      541 gtggcagcgg tgtatggcag cgtagttacc gccggtgtct ataaggcctc ctccattaaa
      601 gtggccgaag cggccaaggt gattgaaaac acccagcggg atttaaatat cgccctgatg
      661 aatgagttgg cgctgatatt tgaccgctta ggtatagata ctttggaagt gttaaaagct
      721 gcgggcacca agtggaattt tttgcccttt cggccgggct tggtgggcgg ccactgtatc
      781 ggtgtggacc cttattattt aacccacaaa gcggaaaaaa ttggctacca cccgcaggtg
      841 atactcgccg ggcggcgcat taacgatggc atgggcgcct atattgccca gcgcgccatc
      901 aaacaattaa tcctctccgg ccactccatt aagggcgctc gcatcggtat tttcggcctg
      961 acctttaaag aaaactgccc ggatttgcgc aattccaaag tgattgacgt gataaacgaa
     1021 tttgcctcct acggggtgga attgttagtg cacgatgcca tggccgacag ccacgaggcc
     1081 gaagaggaat acggtgtgag cctggtggag cttagcgcca tgcagaagct cgatgccgct
     1141 gtgtttgccg tggcccacag cgaatacgcg gctttctcgg ctgcggaggt taaagccagc
     1201 cttaaccccg gcggcgccgt gttcgatgta aaagccatgt ttaacccggc ggatttcgcc
     1261 gccgccggtg ttgcctattg gcgtctataa tcatcagata gtgaatgctg caaaacacaa
     1321 aaacccggtt aagccgggtt tttgtgtttt tgggccacgg aacgctgatg aagccattta
     1381 gcgggtgcaa attaactagc tcatgggtta ataaatcaat gggcaagcgc gcctgcggcc
     1441 agtaaactgc ccgcacctta ctcaacccgc ctgcgacggg tgcaaggtga tgtacaagga
     1501 atggcaaact cctttgccta gaataaattc cacaacccat gcgttggagg caatatgcac
     1561 agactcaaat cacaacccct caatccattc aaccccctgg ccttggccat tgccttaggc
     1621 gccagttgcg gttcggttca agcccaagat gcacctttaa ttgaagtgct ggtcaccgcg
     1681 caaaagcgca gtgaaaatct acaggacgtg cctatttccg tctctgccgt cagcggcagc
     1741 aaactcgatg aggtgggggt tgaaaatatc gaagacctca ccaaactcgt acccaatatt
     1801 catttaaccc aaaccggttt gagcacccag ctgcgtattc gcggtatagg ctccgataat
     1861 agccaggggt ttgagcagtc tgtgggcacc tataaaaacg gtatttacca cggccgcgcg
     1921 caattattgc gcgcgccgat ttttgatatg gaacgcgtgg aagtgatgcg cgggccgcaa
     1981 agcacattat ttggtaaaaa ctctatcgca ggtgccatcg atttaattac cgctcgcccc
     2041 accgatgatt tcagcggttc cttaaatacc agttatgaaa cccagttcgg cacccgggaa
     2101 attaccggta ttgtctccgg ccccttaacc gatagcctca aaggccgctt agccctgcgc
     2161 ggttacgatg acccagggta tttttataat acctttaacc accaacacac caatcagcaa
     2221 caaaccaccg gccgtttgag tgtggattgg cagccgctgc cggaattaac cctgcaatac
     2281 accggcgaaa aaagccgtgc ggattcggcc gggcgcaata tcgaaattgt gcgcgacgac
     2341 ccgaataaaa aaggcctgaa tttcgccacc acatttaaat tgctcacaca aaacaatacc
     2401 cttgaaggta acgccgatta taaacgccag gcagatgcgc cggagtacag cgataacaac
     2461 gtacaaagcc acagcctggt ggctaattat gaattttccg gcctgacgct gacctccacc
     2521 accggcctgg tgaaatacga ttatgaagaa aattgcgatt gcgattttag cagcgtaaat
     2581 ttattccaat taaatctgca cgaagactac aaacaagtca gccaggaagt gcgcctaact
     2641 tccgatcctg gacaaacact ggattggctc gccggggtgt ttttccaaaa ttatcagcag
     2701 gattttggcg atcgtttttc cgtcaacgat aaatcgggct tattccccgt ggctaaagcg
     2761 ggcggccgcc cactgcctgc ggcctttggc aataccggta ttttccgcag ttttgatcaa
     2821 gagtccgata cctgggcggc ctttgctcaa gtggcctggc attttaccga tgccagccaa
     2881 ttaattttag gcggccgcta caccacagaa agcaaagaca gccataaatt aattactatg
     2941 gcgaatatcg atggcgctgc cattaccgat cccgcccgtt tagcctccgc cgcttatgtg
     3001 tataaatcgg tatttaaact cgatacccaa caaacaccct acgcgccttt attaaatgcc
     3061 atggggcaac cggtaattga cccggcaacc aatcggccgc gcccattagt gccgaatcac
     3121 cccggtcacg acttacaagg ctcccgcgat gaaagtgcat ttacgccatc cattaaaatt
     3181 caacacgatt ttaatgacga cctgatggcc tatgccagca tggcgcgagg ttttaaagcc
     3241 ggtggttttg acccgcgctc taacagtaat gagcactatg agtttgagga tgaacaggcc
     3301 gatgcctatg aattgggctt aaaatccacc ctgttggatg gtgcattgga ggtaaatagc
     3361 gctttgttcc gcactgatta tgaaaacctg caaattagcc aatttgatgg cggcgtaggt
     3421 tttaatgtgg gcaatgcgaa agacacccag gtgcaggggg tggaaattga tggccgctgg
     3481 gccatgactc aacacatcat ggccaattac ggcatttcct acctggattt tgaatatcag
     3541 gattttaaaa acggcaactg taataagcca caaattgatg ctggcttagg cgtggatacc
     3601 aatgcggatg gcaaagttga tttgtgtgat tacactggca aacgcggcgt gtacacaccc
     3661 aaatacacgg ttaatacggg cctcacctat aagcgcgaat tggccaatgc caccttcacc
     3721 actgggttgg atgtgcagta tgtaagcagc cagcaggtgc acgttaatct cgaccctcag
     3781 ggcgaaatag acgcctatac cctggtgggg ctgcgcattg cgttagagga cgagcgcttt
     3841 ttagttgcct tgagcgggga aaatctcacc gatgaatatg tgtcgtcgta catggctagc
     3901 gtgcccctgt ccgattccaa cataggctca caaacctttt acggttttgc ccgcaggccg
     3961 cgcacctaca ctttgagtat ggggctgaag ttttaagcgt ttacttttca ccctgttaaa
     4021 aacccagcca catgcggctg ggtttttttt cgactgtaaa aagagctgct cacctaaaaa
     4081 ccgcagtaga gttcagctgc agtttttaag gattaaaagc aaaatccatc acatttaagc
     4141 tcgggcctga gcttaccaat ggcttacctg cgccatatca atgcggcata aatccccaaa
     4201 agagcggtta ccaagcgctt agaaaagact taacgccttt ttttgcgccg aaatcgaaaa
     4261 tcgcacgtgt attttttagc gccgccaact gcgcgtttaa ccttgccgaa tttggcttat
     4321 gcccctgtat ttgctgggct gtagaggcaa tacagggggg gtacgctgtt tgtgccaccc
     4381 gtattttgcc cgagtggccg cagccttgat acgcgctgtt gtttatttca gcgtttatag
     4441 ggcgtatttc ccctgagtgg gcatttcata catctgccga tagaatccaa tagcagcaaa
     4501 gttttatagg tgtttgcgag gcagatgtgt agctgcgcaa aattaaatct gggcgttttc
     4561 cttccggtgg agcgaaagct cagggtacac aataacaatt aataagcgcc gcaagttaag
     4621 caccttgaga gaaagcgcct taagtacaca ccatcaaaag ccgcccagaa tttaaggttt
     4681 tttgacaggc gccctaaagt tcaaccgatc aggttttgat tctataaaaa caacaggctt
     4741 acaaaagtga tgcaattttt gagagtaccc aatgaccttg gcaagctgtt agtgattgcc
     4801 ctgtgcagcg gcgccccttg ggtggcggct gaaagctcaa gccccgagca gttgatgggc
     4861 cagtggctta atttagaaat gcaaaaaggc gccatgcaat ccaactggaa tcgcagccgc
     4921 gataatttgg atcagcgctt tattttgttg gatgccgagc aaaaacgcct caaagaggtg
     4981 ttatcccagc gcactgtggc taccggtgaa gtcgatcagc gccgcttaga gctgctgcag
     5041 cggcaagaaa aattggagca ggagcaggcg caattggatg cacagctaaa aaaatacctg
     5101 caaaaagccc aattgatcca accgcgtttg ccgccgcctt taagcgcgca ttggagcgaa
     5161 aaactcgcgc tactcggcca ggaaaatgcc agcgccagcg agcggttaga acatcttctc
     5221 ggtttattta aacaatttga cgaatttaac cagcgggtgg cgttgcatat tggcgcattg
     5281 gatattcccg acgccaatgc gcacctgcag cccatattaa ccagccaaat ttatttaggc
     5341 gccagccagg gttggtatgt cagtgatgat ggcaaggcct atggctatgg gcggcccacc
     5401 tcaaccggct ggaaatggtg gcacggcgcc gaggcggcaa aagaactcgg ccggccactc
     5461 tctgccgagg caattgcgcg cgtgcggcac atattagaaa accccaccac agccgaattt
     5521 atttctctgc ccgttaaaat ttcacaataa ggaatcaccc cggtgaatcg aacgcacgcg
     5581 ttttctctcc gattatttac gctaattacc ctgttgttgt gctgtgcctc gctggtgcgc
     5641 gctgatgtca acgatcaatt ggtacaaaaa atttcccaag ctaaaaatga actggaagcc
     5701 ctggaaaaga aaaccagcca agagagccgc agttatgccc agcgcttgga acaaaaagaa
     5761 caggaggtta aaaatttacg tagccaggtg gcctcccaac agcgggtggt ggatgaacaa
     5821 ttattggggg tggataaatt aaaagagcgc gttgatcagt gggcggcgca aagccaatat
     5881 caaaaacaat tattaattca ctttaatcaa accgcgaaat tacctgtagc tgtggcaacg
     5941 ggtgacgaat taacgatttt agatcaggct gccaaacagc ttgaacacgc cttagcaccc
     6001 agctggcaag acagtaaagt ggtgtcagcc ggcggcgcta ttattcatgc gccggtaatg
     6061 gccctggggc cggtagaaat tgccgttgat ctacaggcaa aaacagcggg ccccctggtg
     6121 cgtgagaaag atcgcgaagc ttggttgcta gattttctgc cccagtctgc ccaacaggaa
     6181 ctattaaccc tacacagtac ccaggcgggg gaattggcat ttgatccaac ccttggcaac
     6241 gccctgcagc tgcgcaaaca cagcgatggt ttaatggtgt attttcaaaa aggcggtgta
     6301 tgggtgatcc ctattatatt ttttgggttg ctagccttat tgattgcagt attaaaagcc
     6361 gtgcaattgt tgcgtctacc gaaaatagcg cacgacttgg ccgaacaatt taatcaaata
     6421 ataaccgccc ataaacctga tgcccgcgca gcctgtgaag cgctgctggc atccatcggc
     6481 cccgcccagc gcacattggc gctgatcgcc atgaataatc cagtttccca gcagcgcgat
     6541 gatttattgg tggcgcattt atccgagcat aaatatcact tggataaatt catgggcata
     6601 gtggcaacca gtgcttctgt ggcgccactg ttgggtttgc tcggtaccgt ttcgggcatg
     6661 attaatacgt ttaagatgat gacgattttt ggctcgggcg atgttaatac ggtatcgggc
     6721 ggtatatccg aggcgctgat taccactgag ctgggattaa ttgtggcggt gcctgcgtta
     6781 gtgatcggcg ctgtgttgac gcgacaaatt aaaacctatg cccatgggat ggaacgtttt
     6841 gccattaaaa tcagcaaaat tgctttttaa tggaatgccc tgagtatgga taacctgtcg
     6901 cagtctttag cattaatcaa taaccctatt gtgtggatga cgatagccgt ggccattata
     6961 tgttatggct tattgttccg cttttgtttt tgtaaggtgc aggatcagcg ttggtttgaa
     7021 ttaacccgcg attggttgcc gggtataaaa caaatgttaa caactttgcc tctgctgggg
     7081 ctgttgggca ccatcacggg gcttaaagat acgtttattc gccttgccga tggtggcggt
     7141 cttgtattgc aagagttaat taccggcggt attggcgatg cgctgttt
//
LOCUS       DAOQGS010000170         7185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5081_length_7185_cov_4.633520, whole genome shotgun sequence.
ACCESSION   DAOQGS010000170 DAOQGS010000000
VERSION     DAOQGS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5081_length_7185_cov_4.633520"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..786
                     /locus_tag="PKZ52_08070"
     CDS             391..786
                     /locus_tag="PKZ52_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNG59767.1"
                     /translation="MNYDACKLYLLSKPEVMEVYPFGSDSPSFKICHKVFATLSYLHG
                     TGQMNLKCDPDEAERLRDTFDAVVPGHHMNKRHWNTLILNDSIPEEDLKFMMDHSYSV
                     VVDGLPQDQQDGLVARHGYDVIHKGKLKK"
     gene            complement(1096..2250)
                     /locus_tag="PKZ52_08075"
     CDS             complement(1096..2250)
                     /locus_tag="PKZ52_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59768.1"
                     /translation="MGCKQYVGRAVRIQLLGLSVLSAVNIELVCAADAPATVTPGANK
                     RQEQLRLFASFDSVTYAENISIDAWMDDLHTKDTAPGENAFTRNFSEWGVAYGQMSFA
                     GFMRQDYYLHFSQDTFDLVYQDKNKIPFDTNRRYDIYLDVAHVETHGAKVGYDFAPFN
                     SLQTRIEFNVFDAEEVLFGDLTGYLSMVNGKIKGDLLLDYNYTEDVVLDRPKTAPASG
                     RGQSIDLEFWWQPIESLNVHVLAEDIYSRIKWSQTPFTEATITTVRTTTSKDGTVRRM
                     PTISGREYFREVTQTIPRHIQADIGYEFLNRWQIELSQERIDDLAFNRLMARYRIWSQ
                     VKLGLGYDASAQAPRLELSSSYFNLIASADSTDDKKAKFIALSAGLRIAF"
     gene            complement(2438..4303)
                     /locus_tag="PKZ52_08080"
     CDS             complement(2438..4303)
                     /locus_tag="PKZ52_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59769.1"
                     /translation="MDIGCGGTVKFGDKFTLPTNSKLVLETIVPALDKTKSVNVSTFS
                     TLAAEQARKALASNADINALAAAIRKANGEIANMFGLTGELSELELLDVTDAAAVKAA
                     VNGGKSEVLRIAALNAAVISASQSANPGFTLEQALAKLIQDITAKGIASNSSDSTVAD
                     LTKILTAVSNILEQVKKLNASGVDLTALLTSVANELGRVRNEPVDVYNTGSVLPDATL
                     STKAYEFAEVVSQLINGGDIVLDTKVGNSTIKAASNKLRAQWDAAQMLSGPDVEALGK
                     SLSLAAEAIAEAADAYHYDATLKTYTAGDVKVDIKKVDPAVVYTVEQTISGNTVKLVA
                     TSQHTDSTVGTLETAMGKLKLSGSAANAAVKITVQDTSSLEAAKLTRETKNNVETTTL
                     EGLKVTLASDVAQIEVGNVTDPVSASGTLKFEFATFERVKGASGSEPMTKGVMAISFD
                     GKVKNKASEAVAFALNISGNTKGITIDELSQGVDPTDISITEDDFNSLTAAVSFTADL
                     NTQMNSARVKLTTTRESLSSTRTSLDLIFGAYNLRFAALIKRVADQPYGLTISNQDRL
                     TMTLAQPTIGGKYAGKITGPDGVKVLANIEQGKDGCVKVTIVEGGKFICLIDIPD"
     gene            4389..4601
                     /locus_tag="PKZ52_08085"
     CDS             4389..4601
                     /locus_tag="PKZ52_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59770.1"
                     /translation="MVYLPSASVTAVVNGCAETTPLLSKVKADTTPPPTTPFTAAPEM
                     LTVGVSPGGGSITVLVLLLLLLQPQP"
     gene            complement(5346..7184)
                     /gene="dxs"
                     /locus_tag="PKZ52_08090"
     CDS             complement(5346..7184)
                     /gene="dxs"
                     /locus_tag="PKZ52_08090"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNG59771.1"
                     /translation="MESPADLRLLPEKQLPLLARELREFLLYTVGQTGGHFGAGLGVV
                     ELTIALHYVFNTPEDRLVWDVGHQTYPHKILTCRREAMAGIRQAGGIAGFPKRSESPY
                     DTFGVGHSSTSISAALGMALGAAHQGQARKTVAVIGDGAMTAGIAFEALTHAAHAKAD
                     MLVVLNDNNMSISPNVGGLADYFSRIWASKLYTSFRQGSKKLLMRLPQTAEFMRKTEE
                     HMKGMVSPGTLFEELGFYYVGPIDGHDIARLSHDLKNLSEIPGPKLLHIITQKGKGFA
                     PAEQDPVGYHALNKIEPANRAPKPVFKQPRLKYQEVFGQWLCDTAGKDERLIGITPAM
                     REGSGMVAFSEQFPARYYDVAIAEQHALTLAAGLACEGLKPVVAIYSTFLQRAYDQLV
                     HDICIQKLDVTLAIDRAGLVGEDGPTHAGNLDIAYLRCVPELILAAPSDEQECYQLLN
                     TAYHHPGPAAVRYPRGNGPGVPLIPAAAPLAVGVGRQVLSGQQVAILNFGVLLPEAQA
                     IASRRGFALCDMRWIKPLDGELIRQQLAQASLLVTLEEGSIMGGAGSAVAEFLAAEGL
                     VVPLLQLGLPDVNIDHARREQQLARIGLDEAGIERQIDARLQLLGG"
BASE COUNT         1758 a         1877 c         1803 g         1747 t
ORIGIN      
        1 gctgctccat gcgcagccaa gcaaagccgc taaaaaagca aaacgccagt agaataaagc
       61 ccgttaataa accaaataat tgccgcaaac tcatatgtcc catcatcccc ttctcctcgg
      121 gtagcgctcc cttaagctta gtcactatag ggccttacgc cagcggatga gatcggtttc
      181 acggcttagg cgcgcagatg gcttgttgcc ggggcgcgca tggtttaacc ttagcgacca
      241 ttaaaagatc ggcgcgccac cccttccgcc acgcttgcat cgacgcactg aaggcacggc
      301 cctggcgcaa gccctacccg cagggcacaa ccttaacccc ggcgctgcgc gcccccaacc
      361 ctggccactt gcccctaagg ataatcatcc atgaattacg acgcttgtaa gctttatctg
      421 ttgagtaaac ccgaggtcat ggaagtctat cccttcggtt ctgactcccc ctcgttcaaa
      481 atctgccaca aagtgtttgc caccctcagt tacctgcacg gcaccggcca gatgaaccta
      541 aaatgcgacc cggatgaagc cgagcgcctg cgcgatactt tcgacgccgt tgtgccgggc
      601 caccatatga ataaacgcca ctggaatacg ctgatactga acgattccat ccccgaagaa
      661 gacctgaaat ttatgatgga tcactcctac agcgtggtgg tagacggcct gccgcaggat
      721 caacaggacg gcttagtggc ccgccacggc tatgatgtga tccacaaggg taaactgaaa
      781 aaataaacgg ctttaaaacc gctaataaaa aaggctagat gcgtgcatct agcctttttt
      841 attgggtcgt aactgtgcag tatcttttta gtgtaactgg acctgatgcc tgcgccgact
      901 cggaaccatc aggcgagtgc ttcagtgccg cccgcacgca ctgatgaata caggttattt
      961 cagacaatac atccgtgtag gctcatccaa caactaaagc cctacctaag ctaacctaac
     1021 cgaagctaag ctaataaata gctagctgag ccatcattaa cccgctcacc ggttattaac
     1081 ccctttgcaa ggcccttaaa aggcaattcg cagccccgcg cttaaagcaa taaatttagc
     1141 ttttttgtca tcggtgctgt cggccgaagc gatgagatta aaataagaac tgctcagttc
     1201 aaggcgcggc gcttgggcgc tggcgtcata gccaagcccc agcttaacct gtgaccaaat
     1261 gcgatagcgg gccatcaggc ggttaaatgc caaatcgtcg atgcgctctt ggcttaattc
     1321 aatttgccag cggttgagaa actcataacc tatgtcggct tgaatatggc ggggaatggt
     1381 ttgcgtcact tcacggaaat attcccgccc ggaaatagtc ggcatgcggc gcaccgtgcc
     1441 atctttacta gtggtggtgc gtaccgtggt aatagtggcc tcagtaaaag gcgtttgcga
     1501 ccatttaatg cggctgtaga tatcctccgc caacacatgc acatttaaag attctatggg
     1561 ttgccaccaa aactccaggt caatcgattg gccgcgccca gaggcggggg cagtcttcgg
     1621 gcgatcaagc accacatctt cggtgtagtt ataatcgagc aataaatcgc ctttgatttt
     1681 cccattcacc atactgaggt agccagttaa atcgccaaac aatacctctt ccgcatcaaa
     1741 aacattaaat tcaatgcgcg tttgtagact attaaacggc gcaaaatcat accccacctt
     1801 ggcgccatga gtttccacat gggcgacatc caaataaatg tcatagcgcc gattggtatc
     1861 aaagggaatt ttatttttat cttgataaac cagatcaaag gtatcctggc taaagtgcaa
     1921 gtaataatcc tgccgcataa aacctgcaaa actcatctgc ccataggcta caccccactc
     1981 gctaaaattg cgcgtaaagg cattttcacc gggcgcggta tctttagtat gtaaatcgtc
     2041 catccaggca tcaatggaga tattttcggc gtaggtgacg ctgtcgaagg aggcgaataa
     2101 acgcaattgt tcctggcgct tgttcgcacc gggtgtaaca gttgcgggtg catctgcagc
     2161 gcaaacaagc tctatattaa cggcacttaa cacacttaac ccgagcaatt gaatccttac
     2221 tgcccgccct acatactgct tacaacccat tttatacacc tgtttaataa ctctagaaat
     2281 tccgtgtggc tcttatcact atagctggtg gtttttaatt tggcgggttg gcaggtgaat
     2341 aattctaaaa agcaaataga tgaaaataat attttttgaa cgccaacaaa aaagagagcc
     2401 gaagctccct tttgcgaaat caacgttagg tgtggattta atctgggata tcgataaggc
     2461 agatgaattt accaccttca acaatggtaa ctttcacaca accatctttg ccttgttcta
     2521 tattggcaag cactttaacc ccgtcaggcc ctgtgatttt acctgcatat ttaccgccta
     2581 tagttggctg cgccagtgtc atggtcagac ggtcttggtt cgagatagtt aagccgtaag
     2641 gctgatcggc aacgcgctta atcaaggcgg caaaacgcaa gttataggcg ccaaagatta
     2701 aatctaacga cgtgcgggtg gacgataaac tttcacgagt ggtagttaac ttaacgcgcg
     2761 cagaattcat ttgggtattg agatctgcag taaaacttac ggcggcagta aggctattaa
     2821 aatcgtcttc ggtgatcgaa atatcggtag gatcgacacc ctgcgacaat tcatcaatag
     2881 ttataccctt agtgttaccc gaaatattca gcgcaaacgc gactgcttca ctggccttat
     2941 tcttcacttt tccgtcaaaa ctgattgcca tgacgccctt agtcataggc tcagaacctg
     3001 aagcaccttt aacccgctcg aaagttgcaa attcaaattt aagtgtgccg cttgccgata
     3061 cgggatcagt cacgttacct acttcaattt gagcaacgtc acttgctagg gttactttta
     3121 gaccttcaag cgtagttgtt tcaacattat ttttggtttc ccgcgtcaac ttcgcagctt
     3181 ccaagctact ggtatcttgc acggtaattt tgactgcagc gttagctgcg ctaccactca
     3241 gttttagctt acccatggcc gtttccagcg ttcctacagt gctatcagtg tgctgactag
     3301 tggccactaa ttttactgta ttgccagaaa tagtctgctc aactgtgtat acaactgccg
     3361 gatcgacttt tttgatatcg accttcacat cgcctgcagt gtaggttttt aaggtggcat
     3421 catagtgata ggcgtcagca gcttcggcaa tcgcctcagc agccaaggaa aggcttttgc
     3481 ctagcgcctc aacatctggg ccgcttaaca tttgtgccgc atcccattgg gcgcgtaact
     3541 tattggaagc agctttaatt gtgctgttgc caacctttgt atcaagcaca atatcgccgc
     3601 cattaattaa ttggctcacc acttcagcaa attcataagc tttagtgctt aaagtggcat
     3661 cgggcagcac actgccggtg ttgtaaacat ctaccggttc attgcgcacg cggcccaatt
     3721 cattggccac actggtaagc aatgctgtca aatctacacc gctggcatta agttttttca
     3781 cttgctccaa aatattactt acggcggtaa gaattttggt taaatctgca acggtgctat
     3841 cgctactatt actggcgata cccttagcgg taatatcttg aattaatttc gccagcgcct
     3901 gttctagagt aaaaccagga ttggcggatt gggaggcact gatgacagcg gcgtttagag
     3961 cagcgatgcg cagcacttca cttttgccac cattaacagc ggcttttaca gcggccgcat
     4021 cagtgacatc gagcaattca agctcagata attcaccggt aagcccgaac atattggcaa
     4081 tttcaccatt ggctttgcga attgcagcag ccaatgcatt tatatcagca ttagacgcca
     4141 gagcttttct cgcctgctct gcggccagag tggaaaaagt tgatacgtta acgcttttgg
     4201 ttttatccaa ggctggaaca atggtttcca ataccagttt tgaattcgtt ggcaaggtga
     4261 atttatcacc gaatttaacc gtgccgccgc aaccaatatc caagtcacag gtgagcttgg
     4321 tggtatcgtt cgaggctggg gttatgcgga aggaaatcgg tttgcctata taggtttcgg
     4381 gtattttaat ggtgtattta ccgagcgcat ccgtgactgc ggtagtaaat ggctgtgcag
     4441 agaccacgcc tttgttgtcc aaggtaaaag cggatactac gccgccgcca acaacccctt
     4501 ttacagccgc gccggaaatg ctcaccgtgg gggtttcacc cggtggaggt tctattacgg
     4561 tattggtact gttgttatta ctgctacagc cgcagccttg agtcagcgag gcagcgccta
     4621 gagcgcctaa ggctagaagt cgagttacta gattcacgtt tatctcctgg taaattaagt
     4681 gttcgagcct ttcctaatct tgctaaaaac aagactctag tacgcatgta ctccggtcag
     4741 acttgatgcg tggcaatccg caattttgcc tgggcaaaaa aatgcgtcac gatcaaaact
     4801 atagatgcca ggttaacatt cgcaactcgt tcagcttgcg taaacactat ttagccgcca
     4861 aattttacgc gtcaaaaaag ctgcgcttat ttattttttc cgtttcaaat tacgcaccgc
     4921 caataaaatg gcttgtgcct cgcaagagcg gcggaaaatg gcaggtatag aagcactaac
     4981 agcaagaatt tatgcaggag gggaaatagg cgtttagcac gcaaataaca acaggcactt
     5041 ttaaaaagtg aatgaatggt tgggcaatca agttcaatat tgtctatagc ccaattaatt
     5101 ctgcgcaatt ttgcctaaga ggctgctagc agaatttgct attacaaacc ttcttttatt
     5161 cttagttgca attgccaagc tggacatgcg ttagtgtaag caagcgtttt gggcacacct
     5221 aacggctttg tggagaaaca ccagcaatgg aatactaaca atattgtagg aatacgccgt
     5281 agcgcggatg gggctgcgcg attaaactaa gcccatgctt aaagcgccca tggcaagaaa
     5341 cggcgctagc cacccaacag ctgcaagcgg gcgtcaatct gccgctctat gcctgcctca
     5401 tccaacccta tgcgggccaa ttgctgctcc cggcgggcgt ggtctatgtt cacatccggc
     5461 aggcccaact gcagcagcgg caccaccaaa ccctcggcgg ctaaaaattc cgccacggcg
     5521 gagccggcac cgcccatgat gctgccctcc tccagggtta ctaacaggct cgcctgggcc
     5581 agttgttggc ggatcagctc cccatccaag ggcttaatcc agcgcatatc acacagggca
     5641 aagccccggc ggctcgctat ggcctgggcc tccggcagca gtacaccaaa gttgaggatg
     5701 gcaacctgct gcccgcttaa tacctggcgc ccaaccccca ccgccaaagg cgcagcggct
     5761 ggaatgaggg gcacacccgg gccattgccg cgggggtagc gcacggcggc ggggccgggg
     5821 tggtgatagg cggtattcag cagctggtaa cactcctgct cgtcgctggg ggcggcgagg
     5881 attaactccg gtacgcagcg caaataggcg atatccaaat tgcccgcgtg ggtggggccg
     5941 tcttcgccca ccaggcccgc ccggtcgatg gccagggtga catccagttt ttgaatacag
     6001 atgtcgtgca ccaattggtc gtaggcgcgc tgcaaaaagg tggagtagat cgccaccacg
     6061 ggttttaagc cttcacaggc caggcctgcc gccagggtga gggcgtgttg ttcggctatg
     6121 gccacatcgt aataacgggc cgggaactgc tcggagaagg ccaccatgcc ggagccttcc
     6181 cgcatggcgg gggtaatgcc gatcaaacgc tcatccttgc ctgcggtatc gcacagccac
     6241 tggccgaata cctcttggta tttcaggcgt ggctgcttga acacgggttt gggcgcccgg
     6301 ttggccggtt cgattttatt gagcgcgtgg taacccaccg ggtcttgctc ggcgggagca
     6361 aagcctttgc ccttctgggt gatgatgtgc aaaagctttg ggccgggaat ttcgcttagg
     6421 ttttttaaat cgtggcttaa gcgggcgata tcgtggccgt cgatgggacc gacgtaataa
     6481 aagcccagct cctcaaacag ggtgccgggc gacaccatgc ctttcatatg ctcttcagtt
     6541 ttgcgcatga actcggcggt ttgcggcagg cgcatcagca gttttttgct gccctggcgg
     6601 aaggaggtgt agagcttact ggcccagata cgggagaaat aatccgccag cccccccaca
     6661 ttgggggaga tggacatgtt gttgtcgttg agcaccacca gcatatccgc cttggcgtgg
     6721 gcggcgtggg tgagagcttc aaaggcgata ccggcggtca tggcgccatc gccaatcacg
     6781 gcgacggttt tgcgcgcttg gccctggtgg gcggcgccga gggccatgcc gagggcggca
     6841 ctaatggagg tgctggagtg gccgacgccg aaggtgtcgt aggggctttc gctgcgtttg
     6901 gggaagcccg ctatgccgcc cgcctggcga atgcccgcca tggcctcccg gcggcaggtg
     6961 aggattttgt gtggataggt ttgatggccc acgtcccaca ccaggcgatc ttccggcgtg
     7021 ttaaacacat agtgcagggc gatggtcagc tccaccaccc caaggcctgc gccaaagtgc
     7081 ccgccggttt gccccacggt ataaagcaga aactcccgca attcgcgggc taggaggggc
     7141 agttgttttt ccggcaacag gcgcagatcg gcggggcttt ccagg
//
LOCUS       DAOQGS010000171         7138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5118_length_7138_cov_4.719752, whole genome shotgun sequence.
ACCESSION   DAOQGS010000171 DAOQGS010000000
VERSION     DAOQGS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_7138_cov_4.719752"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKZ52_08095"
     CDS             complement(<1..1202)
                     /locus_tag="PKZ52_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNG59772.1"
                     /translation="MNSRQQLLPSRRQIYGWLLLAALLLIIRILAALSPALAPTADTL
                     ALSLCALAGLLVAWDAVSLLRLPSLSIERQCSTVLPVNRWSQVHLAINHACAKPTPVQ
                     ILDGLGAGLVSDQQPCQAALLPQQITRLTYRLKALRRGAFQLQQVFYRIPSPCGLWLR
                     QGAIRLEEQLKVFPDFSAISAFKLLAREQQTNLLGIKKRRRRGEGADFAQLREYRPGD
                     ALRNIHWKATALRQQLISKEYQDAKDQRLIVLLDTGQRMRAQEETLSHFDAALNAAIL
                     LGFIALHQGDHFGLQTFGPTERWVPLQKGTDKVQTLLNSLYDLDAQPEAADFFRAVEL
                     ISQRHLKRSLVVILTNLRSEDQQELTQAVKQLSKKHLVLIGDLQESELQQAAAKPIAN
                     LDDALLYTA"
     gene            1965..5564
                     /locus_tag="PKZ52_08100"
     CDS             1965..5564
                     /locus_tag="PKZ52_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG59773.1"
                     /translation="MRNIFAISLLSALVTACGAGGQQSPNPKPDDGGASSSSSSSSSS
                     STSSTSSSSSSSSSSSSSSNSSTGANSSSSSSGRVSSSSSSSSSSSGRSSSASSSSSS
                     SSSSSSSSSSSSTSSSSSSSSSGRSSSSSSSSSGRASSSSSSSSSSSSSSSSSSSTSS
                     SSSSSSSSSSSSSGSTNTGAIIFEDGFETTALGKFPTGWNYFVEYQQNPAHIDPTRYI
                     TAVSTAKVGSRALLIKGGQQPAMITRALPSGLNKVYIRAWINSKRQIGQSANSNHETL
                     MGLRGDVGSANNEIRFGEIKGVIGTNEVPSDNISPKIDQWGKGPILAANTWHCIEVAF
                     RGDMAYNSLYAYANGQLVHSITAPTDWQNGQLSANWMQNKFKEAIFGWHSFSGFSNEV
                     LVDDVVVSKEPIGCSYQVGGGNASSSSSSSSSSSSSSSSSSSSSSSSSTSSSSGANLY
                     ALGEQNYYRCGNCHGPIGSPEADGMKALARGKTVSQLVSIITTNRLMASANCSGDCAT
                     AVATYVFTNVQNGTGSSSSSSSSSSSSSSSSSSSGRTSSSSSSSSSSSSSSSSSGLVG
                     NSANGKTQYDLQCKSCHGADGIKANGAKYTLSISKYPADMFAELVRKIDEEMPYSSSD
                     PNAWMSCIGQCAVDIATYLQTWRASATECTASQTVTYGARGLKLLTAAEYQASLEDIL
                     GVTTDYASRVVNDSYLGKFPNNSKTQISDSHADKYLDNAQEIASWAVANNKPFACTDT
                     SSCPQKFVSEFLLKAFRRPADSTESAAYTALFTTYGAKNGMTAALTAALTSPQFLYRS
                     EIGVPIRDAVSAGITTGTPNKLAQADQSAYVLTPYEYAAALAYTLTGSTPDDTLLAAA
                     KAGTLTTDAQIQTQVARLLDTTRGKAQVGRFAGIWFNTDSVINEIRDTTLYPKYTASV
                     QKAMAQEVREVFKNIFYDSSATFSELYDANYTFANKELADYYGISGPTGSSFVKVSNL
                     DKRGGITTTGAFLVANAHPDRSSPIQRAVRVRELMLCQHIDPPPNNLAADRTALQMKV
                     EELNKAGTLTTRQFYEINTDHTSCAGCHAKIINPLFGTEDFDQMGLWRSTQLGATGST
                     LNIDDSGVLMGIENPNDGQSVNFNGAKGLGKAIAKLDVTKACFVEKSFRFLINRPYDP
                     AIRDENEPLLSDAEKADYKCVASQAKAAMTSSNNSPRAVFTKLGTSDLLRYRK"
     gene            5632..6930
                     /locus_tag="PKZ52_08105"
     CDS             5632..6930
                     /locus_tag="PKZ52_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG59774.1"
                     /translation="MKDSTTFVNNERRKFFDLVGKAGVAAGILRASPLVAGIFANRYA
                     QAAGTMSKRVIFVYTPDGAPGGMWLPSGSGSSMTLNAATKAYESVKTYCAFRSATMAR
                     GGGHGNTHQVLGTGSYDQDWKGDTLDVQIGTLYGQTSPFANVALGVQSNVGNQGLIGR
                     KSGQAFPSEDSPAAAYKRFFSGATGGSTGGSDPNAQVKSVLELNQQALSSLKTKLGSL
                     EKERLDAHSAALSRISSRLSTTEQPSGACGSVNYNPNGYPTDGGTANAFRGQAELQAE
                     ILVQAMACGLTKVATLQLANHQATWVGHNTTFKNDHHQACHAAGKTEQAEMVNYLSGC
                     IAFLIQRLAATADPVNGGKMIDHTLVVQVSDMGDGADHSAGNAPNMIAGGSGFTTFKR
                     GTAGTAENNEAMLGAVPVLLGIEASVGAGITKFASVASVV"
BASE COUNT         1655 a         2017 c         1904 g         1562 t
ORIGIN      
        1 gctgtgtaga gcagggcatc gtctaaattg gcgatgggtt tagccgccgc ctgttgcaat
       61 tcgctctcct gtaaatcgcc aattaatacc agatgttttt tacttaattg tttgacggct
      121 tgggtaagtt cctgctggtc ttcgctgcgc aaattggtaa ggataactac cagtgagcgc
      181 tttaaatggc gctggctaat taattccacg gcgcggaaaa aatccgccgc ctcgggctgg
      241 gcgtctaggt catagaggct atttaacagc gtttgtactt tatcggtgcc tttttgcagg
      301 ggcacccagc gctcggtagg gccaaaggtt tgcaggccga aatgatcacc ttggtggaga
      361 gcgataaaac ctaacaaaat agcggcgttg agtgccgcat caaaatggct gagggtttct
      421 tcctgggcgc gcatgcgctg gccggtatct aataaaacaa ttaagcgttg gtcttttgca
      481 tcctgatatt ccttgctgat taattgctgc cgcagggcgg ttgccttcca atgaatattg
      541 cgcagggcat cgccgggccg gtattcgcgc aattgcgcaa aatcagcgcc ttcgccgcgc
      601 cgcctacgtt ttttaatgcc caataaattg gtttgttgtt cgcgcgcgag caatttaaaa
      661 gcgctaatgg ctgaaaaatc gggaaacact tttagctgtt cttcaagcct aattgcgccc
      721 tgtcgcagcc aaaggccgca ggggctggga atgcgataaa acacctgctg tagttgaaac
      781 gcaccgcgcc tgagtgcttt aagacggtag gttaagcggg ttatttgctg gggcagaagg
      841 gcggcctggc agggttgttg gtcgctcact aaaccggcgc ctaggccgtc cagtatctgt
      901 accggagtgg gtttggcgca ggcgtggtta atggcaaggt gcacctggct ccagcggttc
      961 accggcaata cagtgctgca ctggcgctcg atgctcaggc tgggcagcct gagcaggcta
     1021 actgcatccc atgccaccag caaaccggca agcgcacaaa ggctcagggc gagtgtatcc
     1081 gccgtagggg cgagggcagg gcttagggcg gcaaggatgc gaattatcaa tagcagggcc
     1141 gcaagcagca gccagccata aatttgtctg cggctgggca gaagttgctg gcggctgttc
     1201 atagggatct ccatgtcata aggatctcca tgtcataggc atctccatgg ggttgattag
     1261 tattgtaggc cggtcgcgac tggatcctgt ctactgatta gcgcctgggc ggcaatgtca
     1321 ttattcgcga gcaaccctag gggcgcccct tttgggtccc gttgcacctt aaatcctagc
     1381 cggtccttga ggctcccaga gggcagccac aagggttgtt cccaggatcc ggccttggct
     1441 cgatgacatt ggcctgggct ggggcaattt aacgccgctt cttcagcgcc aatcaatgta
     1501 ccgcaaactc atcgttctgt ataaatccac tgcaagcggg aaaggcgtcg atttgggtaa
     1561 agctccccaa caccgcagcc taatctgcgc cgcattctac ctagcgcaag cgcaccaccc
     1621 accagcgcta ttgtttttag gctagtccat taagcgtgct gtttacgcct aaagaattag
     1681 ggctgactgg ccgttgctcc tatgacctca gacctatgac tgaaaacgca aaatttattt
     1741 tgcctaatgc ggaatttgaa tatagggtgt gcaaacggta tcacagctta aattttgatc
     1801 aaatgttaaa gtagtcgtat acagttataa cccttggtga aatttaccca ttttggttta
     1861 aggcgcgaag gtgcggcgct ttgtactgag ttttagttaa ttgaggtcgg tgtatcagat
     1921 gagacgttgt tgtttgcggt tgagtacaaa gttgggaggg aagcatgcgt aatatcttcg
     1981 ctatttccct gttatcggcc ctggttaccg cttgtggtgc tggcggccag cagtccccca
     2041 atccaaaacc cgatgacggc ggtgcaagct cgtccagttc cagctcttcg agtagcagca
     2101 cttccagcac tagctcttcg agcagttcat ccagtagcag ctcctccagc agcaactcca
     2161 gcaccggtgc taactcctcc agtagttcgt cgggccgggt gagcagttct agcagctcgt
     2221 cgagcagctc atcggggcgc agcagttcgg caagctccag ttcatcgtca agttcttcca
     2281 gctcatcttc gagttcctcc agctcaacta gctcgtccag ttccagcagc tcctcaggcc
     2341 gcagctcatc tagctctagt agttcatcgg gccgtgccag tagctcctcc agttctagct
     2401 cttcaagctc cagcagttcc tcaagctcta gttccacaag ctccagcagc tcgtccagct
     2461 ccagtagctc gtccagctct agcggcagca ccaataccgg cgccattatt tttgaagatg
     2521 gctttgaaac caccgccctg ggcaaatttc ccaccggttg gaattatttt gttgagtatc
     2581 aacaaaatcc cgcccatata gaccccaccc gttacatcac tgcggtgagc accgccaaag
     2641 tgggcagccg ggcgctgtta attaagggcg gccaacagcc ggccatgatt acccgtgcgc
     2701 tgcccagtgg tttaaataag gtgtacatac gcgcctggat taactccaaa cgccagatag
     2761 gccagtccgc taactccaac cacgagaccc tcatggggct gcgcggtgat gtcggcagcg
     2821 ccaacaatga aattcgcttt ggcgaaatta aaggtgttat aggcaccaac gaagtaccct
     2881 cagacaatat ctcaccgaaa atagatcagt ggggcaaagg ccctatattg gcggccaaca
     2941 cctggcactg tattgaggtg gcctttaggg gcgatatggc ctacaactcg ctgtacgcct
     3001 atgccaatgg tcaattggtg cattccatca cagcgcctac cgattggcag aacggccagc
     3061 tctcggccaa ttggatgcaa aacaaattta aggaagccat ttttggctgg cacagcttca
     3121 gcggatttag caatgaagtg ttagtggacg atgtggtggt atctaaagag cccatcggct
     3181 gtagctacca ggtgggtggc ggcaacgcca gcagctcaag ctcttcgtct tcttcaagtt
     3241 cgtcctcctc cagttcgtca agctcctcaa gctcatccag cagcactagt agttcctccg
     3301 gggccaacct gtacgcattg ggcgagcaaa attattaccg ctgtggcaat tgccacggcc
     3361 ctatcggcag ccccgaagct gacggcatga aagctctggc acgggggaaa acggtcagcc
     3421 agttggtgtc catcattacc actaaccgtt tgatggcctc ggctaattgc tcgggcgatt
     3481 gcgccacggc agttgccacc tacgtcttca ccaatgtgca aaatggcacg ggcagttcca
     3541 gtagctcctc cagcagctcg tccagtagca gttcatccag cagttcaagc ggtcgcacga
     3601 gcagctcatc gagcagcagc tccagtagct cctctagcag ctcaagttct ggcctcgtgg
     3661 gcaacagcgc caacggtaaa actcagtacg acctgcagtg caaatcctgc cacggcgcag
     3721 acggcatcaa ggccaacggc gcgaaataca ccctctcaat cagcaaatac ccagctgata
     3781 tgttcgccga gctggtgcgc aagatcgacg aagaaatgcc ctacagcagt tcagacccta
     3841 acgcctggat gagctgtatc ggccagtgtg cagtggatat tgccacctac ttgcaaacct
     3901 ggcgcgcctc cgccaccgag tgcaccgcca gccaaaccgt tacctacggg gcgcggggct
     3961 tgaagctact cacggcggct gaataccaag ccagtttgga agatatcttg ggcgtcacca
     4021 ccgattacgc atctcgagtg gttaacgata gttacctagg caagttcccc aacaactcaa
     4081 aaacccaaat cagcgacagc cacgccgata aatacctgga taacgcccag gagatcgcca
     4141 gctgggcggt ggccaacaac aagccttttg cctgtacaga caccagcagt tgcccgcaaa
     4201 agtttgtcag tgagtttttg ttgaaggcgt tccgcaggcc cgccgatagc accgagtccg
     4261 ccgcctacac tgcgctcttt accacctatg gggctaaaaa cggtatgacg gctgcgctca
     4321 cggcggcatt gacctcacca cagttcctct atcgctcgga gattggcgtg cccattcgcg
     4381 atgccgtcag cgcaggtatc accaccggga cacctaataa gctggcgcaa gcggatcagt
     4441 ccgcctatgt gttaaccccc tacgaatacg cagcggcgct ggcctatacc ctaaccggca
     4501 gcactcctga tgacaccctc ttggccgctg ccaaagcggg caccctcacc accgatgcac
     4561 aaattcaaac ccaagtggcg cgccttctgg acactacccg aggcaaggca caggtggggc
     4621 gttttgccgg tatttggttt aacacagaca gtgtgattaa tgagatccgc gacaccaccc
     4681 tctaccccaa atacaccgcc agcgtgcaaa aagccatggc ccaagaagtg cgcgaggtgt
     4741 ttaaaaatat cttctacgac agcagtgcaa ccttcagcga gttgtacgac gcgaactaca
     4801 cctttgccaa taaagagcta gcggattact acggtatatc cggccccacc ggcagcagct
     4861 ttgtgaaggt gagcaacctg gataagcgcg gcggcattac taccaccggc gccttcttgg
     4921 tggccaacgc ccacccggat cgctcttcgc ctattcagcg cgccgtgcgc gtgcgtgagc
     4981 tgatgttgtg tcaacacatc gacccgccgc ccaataacct ggccgccgac cgcaccgcac
     5041 tgcaaatgaa ggtggaagaa ttaaacaaag cgggcaccct cactacccgt cagttttatg
     5101 aaattaatac cgaccacacc agttgtgcag gttgccacgc caaaattatc aacccgctgt
     5161 ttggcaccga agattttgac caaatgggcc tctggcgcag cacccaattg ggcgctacgg
     5221 gttctacctt gaacattgat gattcgggtg tgctgatggg gattgaaaac cctaacgatg
     5281 gccagtcggt taatttcaac ggcgctaaag gtttgggtaa agccattgct aagcttgatg
     5341 ttaccaaggc ctgttttgtt gagaagagct tccgcttctt aattaaccgc ccctacgacc
     5401 cagccattcg cgatgaaaac gaaccactcc tgagcgatgc ggaaaaggcc gattacaagt
     5461 gtgttgcctc ccaggctaag gcggctatga cctccagcaa caacagccca cgggccgtgt
     5521 tcaccaaatt gggtacctcg gatttgctgc gttaccgtaa ataaactgca ggcggactaa
     5581 ggccgggcac ccaacagtgc cccgtttccc agatgagtta aaggtttcac tatgaaagac
     5641 agcacaacat tcgtaaacaa cgagcgccgc aagttttttg acctggtggg taaggcgggc
     5701 gttgccgccg gcatcctgcg ggcatcgcca ctggtggcgg gcatttttgc caaccgctac
     5761 gcccaggccg caggcactat gtctaagcgg gtgatctttg tttacacccc cgatggcgcc
     5821 cccggcggca tgtggttgcc cagcggctca ggctccagca tgacgctaaa cgctgccact
     5881 aaggcctatg aaagcgtaaa aacctattgt gcctttcgct ccgccaccat ggcccgcggc
     5941 ggcggccacg gcaacaccca tcaggtgctt ggcaccggca gctacgatca agactggaaa
     6001 ggcgataccc tggatgtaca gataggcacc ctctacggtc aaacctcgcc ttttgccaac
     6061 gtcgccttgg gcgtgcaatc gaatgtgggc aaccagggtt taatcggccg caaatccggc
     6121 caggccttcc ccagtgaaga ctcgccagcg gcggcctaca agcgtttttt ctccggcgcc
     6181 actggcggca gcacgggggg cagcgatcct aacgcccagg taaaaagtgt attggagttg
     6241 aaccagcagg ccctcagctc actgaaaact aaactgggca gcctggaaaa agagcgcctg
     6301 gatgcgcact ctgcggccct cagccggatt tccagccgcc tgagtactac cgagcaaccc
     6361 tccggcgcct gtggctcggt caattacaac cccaatggct accccactga cggcggcacc
     6421 gccaatgcgt ttcgcggcca ggcagagttg caggcagaaa ttctggtgca agccatggct
     6481 tgcggcctta ccaaagtcgc cactttgcag ctggccaacc accaagccac ctgggtgggc
     6541 cacaacacca cgtttaaaaa cgatcatcac caggcctgcc acgcggcggg caaaaccgag
     6601 caggcggaga tggtgaatta tttaagcggc tgtatcgcgt ttttaattca gcgcctggcg
     6661 gcaactgccg acccggttaa cggcggcaaa atgattgacc acaccctggt ggtacaggtg
     6721 agcgacatgg gcgacggcgc cgatcacagc gcgggcaatg caccgaatat gattgctggc
     6781 ggcagcggct ttaccacctt caagcgcggc actgcaggca ccgcagagaa caacgaagcc
     6841 atgttagggg cggtgccggt attactgggc attgaagcga gtgtgggggc gggcattact
     6901 aaatttgcca gcgtggcaag cgtggtttag ccgcttggtt ggcatgcaat aggctgttaa
     6961 aaaagccctc gttagagggc tttttttttg taacgatgca gtacacatta attcgcctaa
     7021 gtaggcggac tcgcagtact cactgtcaaa tcgtttcccg tcagtggggg aattaaatta
     7081 aaaaggcgct gagtagtttc tggcggttct ggaaaatacc aattaatggc aagccgct
//
LOCUS       DAOQGS010000172         7081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5172_length_7081_cov_4.795474, whole genome shotgun sequence.
ACCESSION   DAOQGS010000172 DAOQGS010000000
VERSION     DAOQGS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5172_length_7081_cov_4.795474"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PKZ52_08110"
     CDS             <1..772
                     /locus_tag="PKZ52_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HNG59775.1"
                     /translation="LNPQAGLINLPGGVATLNLPAQFSYLTPAATERLLVEGWGNPPG
                     TKTLGMVIPTEVNPVEAGGWGVVITYEADGHISDADADEINYTDLLKDMQEDNAAANQ
                     ERIKEGYGSMLLIGWAEAPHYDKSSHKFYWAKEFKTDQPGANSLNYNIRVLGRSGVLV
                     LNAVASMEEMPVIRAQMPSLLTATEFVQGNRYEDFNANTDHVAEYGLAALVAGGAAAK
                     LGLFAKLAALFIAFKKLIIIGAVAVGGFIMKLLGRKQK"
     gene            789..1511
                     /locus_tag="PKZ52_08115"
     CDS             789..1511
                     /locus_tag="PKZ52_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNG59776.1"
                     /translation="MIKLLLGLFAAGKFGKIALTCGTMLLSMVTYSFIFGWAYAVGFV
                     LLILVHELGHYGAARQRGLNVGAPTFIPFVGAWIQLKEQPMNVETEAYIGFAGPVTGT
                     IGAMVCYYLAKWQGSDLLMALAYAGCMLNLFNLIPISPLDGGRITAIISPKVWLLGVP
                     LLVVLFFYTHSPMLILIALLAYPQLKHAIWPDKNKLHPEGYYQASFNDRINYGVLYLG
                     LVGFLSVMTYQLHTLLQGRVGN"
     gene            2046..3962
                     /locus_tag="PKZ52_08120"
     CDS             2046..3962
                     /locus_tag="PKZ52_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59777.1"
                     /translation="MKSPLQYLLGFALVASLSAQAADDVNLLETGSGYKRVRLTQDAR
                     NAIFESNTESITEIDTSIKTRSNYDVVIVDTYGGLFTSRIPEWAIMDYLNEKGISRAD
                     AAGPLKDTKIDQVSFDLVALSQLTTSDNRELFPETTTSNEGWLCKKEWKNGSKSVSAS
                     PVNKSQSLDKTLYKNSILDLSTKGNAAATGTISASIEYRTKRNKCIRIPYKAELVKAS
                     FDAKLNLNANPSLVGKALVEYKKNLFNDYVRFWNYSNGWWVYIFYISLDMEVGMTYGA
                     NLNASLAANLDAGYNIAGTLDARYECTKNGCTDVRKISTVELNMPSYLNYSAQLDVAL
                     TPYADLSIQANVDLYKIFEMGDMKLGVAAGLPIRYFGYIGNTCSDANMDGSNETVRAS
                     LLDVTGQSYMYLQYDSFISKKTTKILDVNPFGWTKVQDNITFGPMSGVNPIYVRNFAF
                     KNFGGDDIFNPVLQFTSPLPAANGSIGLAKRSCYPFSAAPVYEVDYGDGIIQRTSSSP
                     VLHNWASTGAKTIRARIVGDAAGRSFNPAQWTTRAAQIATSSSSSSSGGTSTGGKIYE
                     FNDPIKGNTPGCTLTQTYGGGGGGTVYYRYALSCPGHQVTVEKMDTGGNCTFIATPKN
                     YSISGTCTNWRVYN"
     gene            4086..5504
                     /locus_tag="PKZ52_08125"
     CDS             4086..5504
                     /locus_tag="PKZ52_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNG59778.1"
                     /translation="MNKYSLRLAALAASCIFSSASLHAGTRYPLNVYPQIIGVGGPTW
                     QDIVDYKGTQGVSVALLNERARSVGMLRFNDNFGSVTPNNLAGIGLCSGTLISNNQFL
                     IAAHCLVSPDPSIPWPKNADGSPLNQFQSINHMHVRFDYQKGLNGSVMRTVDFPVRKV
                     LEYVISDIDYAIVQLDGSPGATYGFTPISAQIPATNQPLWMVHHPEGDIKKVSFGISK
                     GIQAVDQTADFDRLIYANLDTTNGSSGAGVLNAQGELVGVHSGGKFVNGVARNYGVAI
                     SQIIADEDAEILRTIIGSQRTLLASLINGQTYYASGCGWQQVYSGSGVRVDLLNCAGK
                     PYAQRVNQNYSCQLTATNLGTVEGNCNGYKVYSRPSTSSSSSSSSSSSSSSSSSSSGA
                     NGGRKFYEYGDPIIGNTPGCTLVQTYGAGGGNTVYYRYSLTCPNHQVTIEKTQTNNEC
                     TFAATPKNYSVSGSCANWRVYY"
     gene            complement(5635..>7081)
                     /locus_tag="PKZ52_08130"
     CDS             complement(5635..>7081)
                     /locus_tag="PKZ52_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59779.1"
                     /translation="SRVILTVFRVSTFGGRDQMSAQDPADNSGCPLVVNSAFQLVPMC
                     YGYELKNNLDFDTNKNGRFDDQDAYWNNGKGWLPIGSFNANFSGVFEGNGHAVQHLHI
                     VNPGGRGGLFFGVENAVIRNLALTDFKMQVALGAPLVARMLGGEIKGVYVRGELTCLG
                     ANSASLSGLVGESYYQMSISNALIATDISCANGRISGVGGGEDSLLDKNENRAILVLG
                     QLNSIKNEIFNSYAFANLGLVSQSYWAQDLTQQSEAGVIGATDATLAQLKCPDAANNT
                     SCIPGVTLYENWNQEKNAAGQEYWVFSANNQLPAIRLNDSVYSYQPQVSVNQAPQVRL
                     QFDKNTTVIGGDGKNYFSLRAIFTDADLLNVHALKWDYGALRPSNTLANGYEFYRPAP
                     GKYTVSLRVTDSGKPALTSTAQIVLEFKEDITLVNDPPVVEPNGAAPKNPTAQAGGIT
                     PEVTFWLLLLVFLRGAGGSGKVKNRLASKNI"
BASE COUNT         1934 a         1871 c         1503 g         1773 t
ORIGIN      
        1 gctaaacccg caggcaggcc ttattaacct gcccggcggc gtagccaccc ttaatttacc
       61 cgctcaattt agctacctaa cacccgccgc caccgagcgc ctattagtag aaggctgggg
      121 caacccgccc ggcaccaaaa ccctcggcat ggtaatcccc accgaggtta acccagttga
      181 ggccggtggc tggggtgtag tcattaccta tgaagccgac ggccacatca gcgatgccga
      241 cgcggacgag attaactaca cagatctgct caaagacatg caagaggaca acgccgccgc
      301 caatcaagag cgcataaaag aaggctacgg ctccatgtta ttaatcggct gggccgaagc
      361 gccgcactat gataaaagca gccataaatt ctattgggcg aaagaattta aaaccgatca
      421 gccgggcgcc aactccctta attacaatat tcgcgtattg ggccgcagcg gggtactggt
      481 attaaatgcc gttgccagca tggaagaaat gccagttatt cgcgcgcaaa tgcccagcct
      541 attaaccgcc accgaatttg tgcagggcaa ccgctacgag gactttaatg ccaataccga
      601 ccacgtagcc gaatatggtt tagccgcctt agtagccggt ggcgccgcag caaaattggg
      661 tttatttgcc aaactcgcag cattatttat cgcctttaaa aaactcatta ttatcggagc
      721 cgttgctgta ggcggtttta tcatgaagtt attgggccgc aaacagaaat aaccctaagg
      781 caacagccgt gattaaatta ttattaggtt tattcgccgc cggtaaattc ggcaaaatcg
      841 ccctcacctg cggcaccatg ctgctatcga tggtgaccta ttcatttata ttcggctggg
      901 cctatgcggt ggggtttgta ctgctaattt tagtgcacga actcggccac tacggcgccg
      961 cccgccaacg gggtttaaat gtgggcgcgc ccacctttat tccttttgtc ggcgcctgga
     1021 ttcaattaaa agaacagccc atgaatgtgg aaactgaagc ctatataggt tttgcaggcc
     1081 cagtcaccgg caccatcggc gccatggttt gttattattt agccaaatgg cagggcagcg
     1141 atttattaat ggccctcgcc tatgctggct gcatgttaaa cctatttaat ttaattccta
     1201 tctccccttt agacggcggc cgcattaccg ctattatctc ccctaaagtg tggctgctgg
     1261 gtgttccctt attagtggtt ttattttttt acacccacag ccccatgtta atcctcatcg
     1321 cccttttagc ctacccacag cttaaacacg ccatctggcc cgacaaaaac aaactccacc
     1381 cggagggcta ttaccaagcc agttttaacg accgcattaa ttatggtgtt ttatatttgg
     1441 ggttggtagg gtttttaagt gtgatgacct atcagctgca tacgctgttg caggggcggg
     1501 ttggcaacta atttatactc gccccttgat atttatctgc accattaatt acagcaccgt
     1561 taaaacaaaa cattctgcct gtcgcgggta accggctgaa aagccttgta tgctcgcaac
     1621 ttcaggccta acagcctgtt gaaatacctg ggcggcaatt acaggttagc attcactgct
     1681 aagctacagg tatgacatgc ctattgatcg ccgccgtctt ctacacccca cacatctgca
     1741 tcaacccacc taaatttggg atccaactgc ccatcgcttt gtcaagcata caaaaatgca
     1801 tgtggcctat acatatttgt taattgtctg caccctgcgg tttcgttagg attccgccgc
     1861 cgtctcaagg cgcgcatcta tctgcattaa tttaaataga tgcgttgttc agcttcacac
     1921 tagtgctagc tcaattacac agcactagct tgcatattac cgagtgcgta tagcgcatta
     1981 ctcatttaaa caatcggcgc cacaggcatt gtttacttct attttctacc accaggagac
     2041 tcgatatgaa atcacctctt cagtacttat tagggtttgc actcgttgca agcctcagtg
     2101 cacaagcggc agatgatgta aatctgctcg aaactggctc aggctataaa cgcgtgcgcc
     2161 taacacagga tgcacgaaac gctatatttg aaagcaatac agaatccatc accgagatcg
     2221 acacatccat taaaacgcgc agcaattatg acgtggttat agtggatacc tatggtggtt
     2281 tatttacttc tagaatcccc gaatgggcga ttatggatta cttaaatgaa aaaggtattt
     2341 ctcgcgctga tgccgcaggc ccactgaagg atactaaaat cgaccaagtg agctttgact
     2401 tggtcgccct cagccaactg acaaccagcg ataaccgcga attatttcct gaaaccacca
     2461 ccagcaatga aggctggtta tgtaaaaaag agtggaaaaa cggcagcaaa tccgtctccg
     2521 cttcacctgt caacaaaagc caatcgctag acaaaacact ctataaaaac tccatactgg
     2581 atttatccac caagggtaat gccgcagcta cgggtaccat aagcgcttcc attgaatatc
     2641 gcaccaagcg caacaaatgt atccgcatcc cctataaagc tgaattggtt aaagccagct
     2701 tcgatgccaa actcaatctc aatgccaacc caagcctggt gggcaaagcc ctggttgaat
     2761 ataaaaagaa ccttttcaac gactacgtcc gcttttggaa ttactccaac ggctggtggg
     2821 tttatatttt ctatatttcg ttagatatgg aagtaggtat gacctacggt gccaacttaa
     2881 atgcttcatt agccgccaac ttagatgcag gctacaacat tgcaggcacc ttagatgctc
     2941 gctatgaatg taccaaaaat ggttgtactg atgtgcgcaa aatcagcacc gttgagctga
     3001 atatgccttc ctatttaaat tattccgctc agttagacgt agccttaacg ccctatgccg
     3061 acctctccat ccaggccaat gtggatttat ataaaatatt tgaaatgggc gacatgaaac
     3121 ttggcgtggc cgcaggctta cccattcgtt attttggcta tataggcaat acctgctccg
     3181 acgccaatat ggacggcagc aatgaaaccg tacgcgcttc gctgctggat gttaccgggc
     3241 agtcttatat gtatttgcaa tacgacagct ttatctccaa aaaaaccacc aaaattttag
     3301 acgtaaatcc cttcggctgg accaaagtgc aagacaatat aacctttggc ccgatgtctg
     3361 gcgttaaccc tatttacgtg cgcaatttcg cctttaaaaa cttcggtggc gacgatattt
     3421 ttaacccagt tctgcaattt acctcaccac tgcccgccgc caatggcagc ataggccttg
     3481 ccaaacgctc atgttatcca tttagcgcag cgccggttta tgaagtggat tatggcgatg
     3541 gcattattca acgcaccagc agctcacctg ttctgcacaa ctgggctagc acaggtgcga
     3601 aaaccatccg tgcgcgtatc gttggcgacg ccgcaggccg cagttttaac cccgcacaat
     3661 ggacaacccg cgccgcacaa attgccactt catccagctc gtcaagttca ggtggcacca
     3721 gcacaggtgg aaaaatttat gaatttaatg accccattaa aggcaacact ccaggctgca
     3781 ccctgacaca aacctatggc ggcggcggtg gcggtaccgt ttattatcgc tacgccctca
     3841 gctgccccgg ccaccaagta acggttgaaa aaatggacac tggcggcaac tgtacattta
     3901 ttgccacacc gaaaaattac agtatttcag gtacctgcac caattggagg gtatacaact
     3961 aaacaattaa accggcatta acccagcctc aaaaactggg ttaatgccct gtatcaacct
     4021 gcataaaatt tatgccggtc aattacacct caacacattc aaccctaaat aacagcggaa
     4081 ttcttatgaa caaatattcg cttcggcttg cagcgctagc agcgagctgt atattttcca
     4141 gtgccagcct acacgccggc acccgctatc ccctcaatgt ttatccacaa attatcggcg
     4201 tcggcggccc cacctggcag gatatagtgg actataaagg cacccagggc gtcagtgtgg
     4261 cgctacttaa tgagcgcgca cgctcggtgg gcatgctgcg ctttaacgat aattttggca
     4321 gcgttactcc caacaactta gctggcatag gcctgtgtag cggcacgctc atcagcaata
     4381 atcaattttt aattgccgcc cactgtttgg tttcacccga ccccagtatt ccgtggccca
     4441 aaaatgccga tggcagcccg ctcaatcaat ttcaatccat taaccacatg cacgtgcgct
     4501 ttgattatca aaaagggctc aacggctcag tgatgcgcac cgtcgatttt ccggtgagaa
     4561 aagtattgga atatgtcatc agcgatattg attacgcgat tgttcaactc gatggcagcc
     4621 cgggcgcaac ctatggcttc acacccatca gcgcacaaat tcccgctacc aatcaaccct
     4681 tgtggatggt gcaccacccg gaaggcgaca ttaaaaaagt ttccttcggt atctccaaag
     4741 gcattcaagc ggttgaccaa acagctgatt ttgaccgttt aatttacgcc aatttagata
     4801 ccaccaatgg cagctccggg gccggggttt taaatgcaca gggcgaactg gtcggcgtgc
     4861 acagcggcgg taaatttgtg aacggcgtag cccgcaacta cggagtagcc attagccaaa
     4921 ttattgctga tgaggacgca gaaattctgc gcactattat tggcagccag cgcacattat
     4981 tagcctccct gattaacggc caaacctact atgcaagtgg ctgtggctgg caacaggttt
     5041 actctggctc aggcgttaga gtagatctac tcaactgcgc aggaaaaccc tacgcacaaa
     5101 gagttaacca aaactacagc tgtcaattaa cagccactaa tttaggtacc gtcgaaggta
     5161 attgcaacgg ctacaaagtg tattcgcgcc ccagcacctc ttcatcaagc tcatccagca
     5221 gctcgtcgag cagctcttca agctcatcaa gttccggcgc taacggcggt aggaaattct
     5281 acgaatatgg cgacccaatt attggtaaca cacccggctg tactttagta caaacctacg
     5341 gtgccggtgg cggcaacacg gtttattacc gctatagcct cacctgccct aatcatcagg
     5401 taactattga aaaaacccaa accaacaatg agtgtacatt tgccgcaaca ccgaaaaact
     5461 acagtgtttc aggcagctgc gctaactggc gggtttatta ttaatattat ttcataatct
     5521 caacacctcg atcttagcgc gataaacagc gctaagatcg caatgccaga tatgtaaagt
     5581 aaccatcatc aacagagagc atcgaagtaa caacaggcgc atactcaagc tgtgctagat
     5641 gttcttgcta gctaatctat tcttgacctt gccacttcca ccagctcccc gcaagaatac
     5701 aagcaacaat agccaaaaag ttacttcagg agtaattcca ccggcctgtg cagtcggatt
     5761 ttttggggcg gccccatttg gttcaactac tggaggatca ttaaccaaag taatatcttc
     5821 tttaaactcc aaaacgattt gcgcagtgct agttagggca ggttttcctg aatctgtcac
     5881 ccttaagcta accgtgtatt tacctggcgc cggtcggtaa aactcatagc cattcgctaa
     5941 agtattgctc gggcgcaagg cgccataatc ccatttgagt gcatgcacat taagcaggtc
     6001 ggcatcggta aaaatagccc ggagactaaa atagtttttg ccatcgccgc caattactgt
     6061 tgtattttta tcaaactgga gccgaacttg aggcgcctgg ttaacggata cttgcggttg
     6121 atatgaatag acagaatcgt ttagccttat cgcgggcagc tgattattgg cggaaaacac
     6181 ccaatactcc tgtcccgctg cattcttctc ttggttccaa ttttcataca aggtaacccc
     6241 aggaatacaa ctggtgttat tcgctgcatc ggggcatttt agctgcgcaa gcgtagcatc
     6301 agttgcgcca attacaccgg cctcactttg ctgagtaaga tcctgcgccc agtaggattg
     6361 gcttaccagc cctaagtttg cgaaggcata gctgttaaat atttcatttt ttatagaatt
     6421 tagctgtccg aggactaaaa tagccctatt ttcattcttg tctaaaagag aatcttcgcc
     6481 cccgccaaca cctgaaattc ggccgttggc gcaactgata tccgtcgcga taagcgcgtt
     6541 gctaatgctc atctggtaat aactttcacc aaccaaacca ctcaaagatg cagaattggc
     6601 gcccaaacac gttaattcac cgcgaacata tacccctttt atttcgccac ccaacattct
     6661 tgctaccaaa ggtgcaccca gtgcaacctg cattttaaaa tccgttagcg ccaaatttcg
     6721 gatgacggca ttttccacac caaaaaacaa gccgccacgg ccacctgggt tcactatgtg
     6781 taggtgctgc acggcatgac cattgccctc aaagacaccg ctaaaattcg cattaaaact
     6841 accaatgggc aaccaacctt taccattatt ccaataggca tcctgatcat caaaacgacc
     6901 atttttgttg gtgtcaaaat ccaaattatt ttttaattca tatccgtagc acataggcac
     6961 caattgaaat gcagaattaa cgaccaatgg gcagccgctg ttatcagctg ggtcctgagc
     7021 cgacatttga tccctaccac caaaagtgga caccctaaaa acagttagaa taacccgaga
     7081 g
//
LOCUS       DAOQGS010000173         7064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5186_length_7064_cov_4.428449, whole genome shotgun sequence.
ACCESSION   DAOQGS010000173 DAOQGS010000000
VERSION     DAOQGS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5186_length_7064_cov_4.428449"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKZ52_08135"
     CDS             complement(<1..231)
                     /locus_tag="PKZ52_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNG59780.1"
                     /translation="MIELLLQSPWLSSLVALAALCFICGLLLGFAALRFKVEGNALVE
                     QIDALLPQTQCGQCGHPGCRPYAEAIAAGEAIN"
     gene            complement(252..824)
                     /gene="rsxA"
                     /locus_tag="PKZ52_08140"
     CDS             complement(252..824)
                     /gene="rsxA"
                     /locus_tag="PKZ52_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNG59781.1"
                     /translation="MEFFTILLGMVLVNNFVLVQFLGLCPFMGVSNQTTSALGMGAAT
                     TFVLTLASLSCHLVDHYLLVPLHIPFLRTISFIVVIAAVVQFAKLFIEKFSPVLYRVL
                     GVYIPLITTNCAVLGLCLQNASKALNFFQAGVYGLGAGLGFSLVLVCFSALRERIAQA
                     DSPQAFKGPALAMITAGLMSLAFMGFAGLV"
     gene            complement(829..2856)
                     /gene="metG"
                     /locus_tag="PKZ52_08145"
     CDS             complement(829..2856)
                     /gene="metG"
                     /locus_tag="PKZ52_08145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNG59782.1"
                     /translation="MSVRTILVTCALPYANGSIHLGHMVEHIQADIWVRFQKMRGQQI
                     TFVCADDAHGTAIMLAAEKLGISPEEQIERVKAEHMADFAGFSIGFDNYHSTHSAENK
                     TLSELIYARLKANGHISERNIVQAFDPVKNIFLADRYIKGTCPKCKTPDQYGDNCEAC
                     GATYAPMELINPVSVYSGATPIAKESKHLFFTLPAFSDFLRDWTRAGHLAPEVANKLA
                     EWIEGGLQEWDISRDAPYFGFEIPGEAGKYFYVWLDAPIGYMASHQHYCQQHNLDFNA
                     YWSADSRAELYHFIGKDIINFHGLFWPAMLHSANFRTPTQVFCHGFLTVDGKKMSKSR
                     GTFVNARTYLEHLNPEYLRYYFASKLTAGVEDIDLNLEDFINRVNSDLVGKVVNIASR
                     CANFIHKFNDGQLNSTPIDLPLWKRVQEASQPIAQLYEAREFGKAMREIMALADAANE
                     FIAANAPWALAKQAGQEQQVIAICSLGINLFAALMTYLKPVLPTMAHEAEEFLGVTLN
                     WQTPPQYLHGQTIKPFKPLLQRVEGEKVQAMIEASKPLEAAAEAPVAAASGWLATDPI
                     APQIDYADFAKIDLRVARIVHAEAVKGADKLLRLELSLGDEQRQVFAGIKSAYAPEDL
                     IGKHTVMVANLAPRKMKFGMSEGMVLAAGPGGKDIFILEPHPGAEPGMKVM"
     gene            complement(2983..3780)
                     /gene="cpdA"
                     /locus_tag="PKZ52_08150"
     CDS             complement(2983..3780)
                     /gene="cpdA"
                     /locus_tag="PKZ52_08150"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HNG59783.1"
                     /translation="MNTKPLRLLQLTDPHLGALADETLIGLNTEESLKGVLTLIRDSG
                     ITYDLIIGTGDISNDGSIASYQRFVDRVREYIPNVPLAWLPGNHDNPKNMATFPRQAV
                     WIEDFSLGQWQFLLLNSRIPFEEGGELSPGELARLERALSLRPERNTLIMLHHQPVPV
                     GSEWIDQYVVKNAPQFFAILERFPNVKAVSWGHVHQAFEAVRGKLKLFASPSTCVQFA
                     PNCDHFQVDHLMPGCRSYLLHPDGDIESTVLRAKDRIYCIDYSSTGY"
     gene            complement(3864..4343)
                     /locus_tag="PKZ52_08155"
     CDS             complement(3864..4343)
                     /locus_tag="PKZ52_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HNG59784.1"
                     /translation="MTAVSHRAIGQRHFSVNLKDHHAQCELNFHRFLHLLPGCRTGRE
                     LWCYFLGANRQHKISIRLIESAPYTSTLEVSQQQTGGMVFSHGTWRIRLYHDVEMAEV
                     VAWDRHQHWLPQYDYPNAKMYQPDEKLALNRFLGEWLEHCRKQGICPDNIVIKFTNM"
     gene            complement(4343..4960)
                     /locus_tag="PKZ52_08160"
     CDS             complement(4343..4960)
                     /locus_tag="PKZ52_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNG59785.1"
                     /translation="MSFEAQFTQQDVEVVAVEPIFQGFFTYTKQHFRHRRFNGGWSGV
                     FEREMLIKQEAAGAILYDPVHDLIGLVEQFRAGAMESEFGPWCLELVAGITEPGEPIE
                     ALVQREISEEAGVSEAKLIPITQYYSTPGASNEKIHLFCGLADLSSAGGVHGLEEEHE
                     DILFSVYPAEEVFAAMLNSRMNNAATLIALLWLQLNRPNLRTGNP"
     gene            complement(4962..6866)
                     /gene="thiC"
                     /locus_tag="PKZ52_08165"
     CDS             complement(4962..6866)
                     /gene="thiC"
                     /locus_tag="PKZ52_08165"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNG59786.1"
                     /translation="MSYTPDTQFVSQNAKVDEASVKPFPRSQKIYVTGSRDDIRVPMR
                     EISLFDTPTDMGGEQNPPVRVYDTSGPYSDPNVIIDLRQGLPDIRSQWILDRADTEVL
                     TGSASDFTRQRLDDPALQKLRFAHLRAPRQAKAGMNVSQMHYAKKGIITPEMEYIAIR
                     ENMSLQQAKEQGVLSQQHAGMSFGANIPKEITPEFVRQEVALGRAIIPANINHVELEP
                     MIIGRNFLVKINGNIGNSVVTSSIEEEVAKLTWGIRWGADTIMDLSTGKNIHETREWI
                     IRNSPVPIGTVPIYQALEKVGGIAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYV
                     PLTAKRTTGIVSRGGSIMAKWCLAHHKENFLYTHFDDICQIMKAYDVSFSLGDGLRPG
                     SIADANDAAQFGELETLGELTQIAWQHDVQTMIEGPGHVPMHMIKENMDKQLEVCKEA
                     PFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKDDVKEGII
                     TYKIAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQFNLGLDPDTAREYHDETLPKD
                     SAKVAHFCSMCGPKFCSMKITQDVRDYAASLGQQLGNEETVRMLDVEAEMKAKADEFR
                     AQGSQIYSRV"
     gene            complement(6872..>7064)
                     /locus_tag="PKZ52_08170"
     CDS             complement(6872..>7064)
                     /locus_tag="PKZ52_08170"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNG59787.1"
                     /translation="AAEHSHGSGCTLASAIAAGLAQGLNLRQAIAAAKTFIAGALEQS
                     ARLQLAPSNGPIHHFYAYW"
BASE COUNT         1628 a         1791 c         1988 g         1657 t
ORIGIN      
        1 gttgatggcc tcccccgccg ctatggcctc ggcgtagggg cggcagccgg ggtggccgca
       61 ctggccgcac tgggtttgcg gcaacagggc atcgatctgt tctaccaggg cattgccctc
      121 caccttaaag cgcagggcgg caaagcccag cagcagcccg caaataaaac acagggcggc
      181 cagggcaacg aggctgctga gccaggggga ttgtaagagc aactcgatca tattgggcta
      241 gcggggcagg cctaaactaa gcctgcaaac cccataaaag ccaacgacat cagccctgcg
      301 gtaatcatgg ctagggccgg gcctttaaag gcttgggggc tatcggcttg agcgatgcgc
      361 tcgcgcaggg cggaaaaaca cactaaaacc agtgaaaatc caagcccggc gcccaagcca
      421 tagacaccgg cctggaaaaa atttaaggcc ttagaggcat tctgcaaaca caagcccagc
      481 accgcgcaat tggtggtgat cagcgggata taaacaccga gcacgcgata taacactggg
      541 ctgaattttt cgataaataa tttggcgaat tgcaccaccg cagctataac cacaataaaa
      601 ctgatggtgc gcaaaaacgg aatgtgcaat ggcaccagca gatagtgatc caccagatgg
      661 caacttaacg aggccagggt taatacaaaa gtggtggccg cccccatgcc gagtgcggag
      721 gtggtttggt tagagacacc cataaacggg cacaggccta aaaattgtac caagacaaaa
      781 ttattcacca gcaccatgcc taacagaatg gtaaaaaact ccacggcttt acattacctt
      841 catgccgggc tcggcgcctg ggtggggttc aagaataaat atatctttgc cgccggggcc
      901 tgccgctaac accatgcctt cgctcatgcc aaatttcatt ttacgcggtg ctaaattcgc
      961 caccatcacc gtgtgtttgc caattaaatc ttccggtgca taggcacttt taattccggc
     1021 aaatacttgg cgctgctcat cgccaaggct taattccaag cgcaataatt tatcggcgcc
     1081 ttttaccgct tcggcgtgga ctattctggc aacccgcaaa tcgattttgg caaaatccgc
     1141 atagtcaatt tgcggcgcta tgggatctgt tgccaaccaa ccgcttgcag cggcgacggg
     1201 cgcctctgcc gccgcttcaa gcggtttact cgcttcaatc atggcttgaa ccttctcgcc
     1261 ttccactcgc tgcagcaagg gtttaaaggg tttaatggtt tggccgtgta aatattgcgg
     1321 cggggtttgc cagttgagtg tcacccctaa aaattcttcc gcctcatggg ccatggtggg
     1381 caacacgggt ttaaggtagg tcatgagggc ggcaaataaa ttaatgccga ggctgcaaat
     1441 agctatgacc tgttgttcct ggcccgcttg tttggccagg gcccagggcg cattggcggc
     1501 gataaattcg ttggcggcat cggcaagggc cataatttcc cgcatggctt tgccaaattc
     1561 ccgcgcttca tacaactgcg caataggctg gcttgcttcc tgtactcgct tccatagggg
     1621 taaatcaatg ggtgtgctat ttaattggcc gtcgttaaat ttatggataa agttggcgca
     1681 gcggctggcg atattcacca ccttgcccac caaatccgaa ttcacgcggt tgataaaatc
     1741 ttccaggttt aggtctatat cctcaacacc ggcagtgagt ttgctggcga agtaataacg
     1801 caaatattcg gggtttaaat gctcaaggta ggtgcgggca ttaacgaatg tgccgcggga
     1861 tttactcatt tttttgccgt ccacggttaa aaagccgtgg caaaacactt gggtcggcgt
     1921 gcggaagttc gccgagtgca acatggcggg ccaaaataaa ccgtggaaat taattatgtc
     1981 tttgccaata aagtggtaca gctcagcgcg gctatcagcg ctccaatagg cattaaaatc
     2041 taaattgtgc tgctggcaat agtgttggtg ggaggccata tagccaattg gcgcatccag
     2101 ccacacataa aaatatttac ccgcttcgcc gggaatttca aaaccaaaat aaggcgcatc
     2161 gcgggagata tcccattctt gcaaaccacc ctcaatccat tcggcaagtt tattggccac
     2221 ctccggcgct aaatgcccgg cccgcgtcca atcgcgcaaa aagtcgctaa atgcgggtag
     2281 ggtaaaaaac aagtgcttgg attctttggc gatgggggtt gcgccggaat atacggacac
     2341 aggattaatt aattccatag gcgcataggt ggcgccacag gcttcgcagt tatcgccgta
     2401 ttggtcgggg gttttgcatt tagggcaagt gcccttgata taacggtcgg ccaaaaaaat
     2461 attttttacc gggtcaaagg cctgcacaat gttgcgctcg ctgatgtggc cattggcttt
     2521 taagcgcgca taaattaact cactgagggt tttattttcc gccgaatggg tggaatggta
     2581 attatcaaag ccgatagaaa agcctgcaaa atcggccatg tgttcggcct ttacgcgctc
     2641 aatttgttct tctgggctga tgccgagttt ttccgccgcc agcataatag cggtgccgtg
     2701 ggcgtcgtcg gcgcatacaa aggtaatctg ttggccgcgc attttctgaa aacgcaccca
     2761 aatatccgcc tggatatgtt ccaccatatg gcctaaatga atagagccat tggcataggg
     2821 gagggcacag gttacaagaa tggtacgcac ggacatgaaa aaaacacctt taaggccggg
     2881 aaaaagccgc gactatagca gcttgggcct tggcggtcat gcctagatga ggggtgaagg
     2941 gccgccggat gcctgccgaa tagactggac tctggggctg agttagtagc cggtggagct
     3001 gtaatcgatg cagtagatac gatctttggc ccgtaaaaca gtcgattcaa tgtcgccatc
     3061 tgggtgcagt aaataactgc ggcagccggg cattaaatga tcgacctgga aatgatcgca
     3121 attgggggca aattgcacgc aggtggaggg gctggcaaat aatttcagtt tgccgcgcac
     3181 ggcttcaaaa gcctggtgca catggcccca ggagacggct tttacgttgg ggaaacgctc
     3241 cagaatggca aaaaactggg gggcattttt aaccacgtat tgatcgatcc actccgaccc
     3301 caccggcacc ggttgatggt gcagcataat aagggtattg cgctcgggcc gcaggcttag
     3361 ggcgcgctct aaacgggcca gctcacctgg ggagagctcg ccgccctctt caaaaggaat
     3421 acgggaattg agcagcaaaa actgccattg gcccagggaa aaatcctcaa tccacaccgc
     3481 ttggcggggg aaggtggcca tatttttggg gttgtcgtgg ttgcccggca gccaggctag
     3541 gggcacattg gggatatatt cgcgcacccg gtccacgaag cgttgatagg aggcgatgct
     3601 gccatcattg gaaatatccc cagtgccgat gatcaggtcg taggtgatcc cgctgtcgcg
     3661 aatcagggtc agtacgccct ttaaactctc ctcggtgttt aaaccgatga gggtctcgtc
     3721 agccagcgcg ccgaggtgag gatcggtgag ctgtagtaat cgcaagggct tggtattcaa
     3781 tctaacgact ctctccatcg ttgcctaccg aacttctttt aatgtaagtg tgcagtggaa
     3841 acggcacttg gctaatggaa tgttcacatg tttgtgaatt taatcacaat attgtccgga
     3901 caaataccct gtttgcgaca atgttctaac cattcaccca aaaaccgatt gagggccagc
     3961 ttttcatccg gttggtacat cttggcgttg ggatagtcgt attggggcag ccagtgttgg
     4021 tggcgatccc aggcgaccac ttccgccatt tccacatcgt ggtacaggcg aatgcgccag
     4081 gtgccatggc tgaataccat acccccggtt tgttgctggc tgacttcaag agtagacgtg
     4141 tatggagctg attcaattaa ccggatagat attttgtgct ggcgattggc gcctaagaag
     4201 taacaccaga gttcccggcc ggtgcggcag ccgggcaata aatgcaggaa acgatggaag
     4261 ttaagctcac attgggcgtg gtggtcctta aggttcaccg aaaaatggcg ctggccgatg
     4321 gcccgatggg ataccgcagt catcaggggt ttcctgttct aaggttgggg cggttaagtt
     4381 gcaaccataa tagagcaatt agggttgccg cgttattcat tctactgttg agcatagcag
     4441 cgaagacctc ctctgccggg tacacgctaa aaagaatatc ctcgtgttcc tcctccaggc
     4501 cgtgaacccc tccggctgag cttaaatcgg ctaggccgca gaataaatga attttttcgt
     4561 tactcgcgcc gggggttgaa taatattggg taatcgggat gagtttggct tcgctaacgc
     4621 ctgcctcttc actgatttcc cgctgcacca acgcctcgat aggctcaccc ggttccgtaa
     4681 tccctgctac cagctccaaa caccaggggc caaattcaga ctccatagcc ccggcgcgaa
     4741 actgctctac caggccgatg aggtcatgca ccgggtcgta caaaatagcc cctgcggcct
     4801 cttgtttgat gagcatctcc cgctcgaaca caccgctcca gccgccgtta aaacggcggt
     4861 ggcgaaagtg ttgtttggta taggtaaaaa agccctggaa gataggttcc acggcaacga
     4921 cttctacatc ctgttgggta aattgcgctt caaaagacat attaaacccg gctgtaaatc
     4981 tggctgccct gggcgcggaa ctcatcggct ttggctttca tttccgcttc cacatctagc
     5041 atgcgcacgg tttcttcgtt gccgagctgt tggccgaggc tcgcggcgta atcgcgcacg
     5101 tcctgggtga ttttcatgga gcaaaactta ggcccgcaca tggagcaaaa atgggcgact
     5161 ttggcggagt ccttcggcag agtttcatcg tggtattctc gcgccgtgtc tgggtccagg
     5221 ccgaggttaa attggtcctc ccagcgaaac tcaaagcgcg cttttgatag agcgttatcc
     5281 cgcaactgag cccccggatg gcccttggcc agatccgctg cgtgggcggc gattttatag
     5341 gtgatgatgc cttctttaac gtcgtcttta ttgggcaggc ctaaatgttc tttgggggtt
     5401 acataacaga gcatggcaca gccgaaccag ccgatcatag ccgcgccaat gccggaggta
     5461 atgtggtcgt agccgggggc tatgtcagta gtcaagggcc caagggtata gaagggggcc
     5521 tctttacaca cctctaactg tttatccatg ttctctttga tcatgtgcat gggtacgtgg
     5581 ccggggcctt cgatcatggt ttgtacatcg tgctgccaag caatttgggt aagctcaccg
     5641 agggtttcca gctcgccaaa ttgcgccgcg tcattggcat cggcaatgga gccggggcgc
     5701 aggccgtcgc ctaaggagaa ggacacgtca taggccttca tgatctggca gatgtcgtca
     5761 aagtgggtgt aaaggaaatt ctctttgtgg tgggctaaac accatttggc catgatggag
     5821 ccgccgcggc tgacgatacc ggtggtgcgt ttggcggtga ggggtacgta gcgcagcaga
     5881 acaccggcgt ggatggtgaa ataatccacc ccttgctcgg cctgctcgat tagggtatcg
     5941 cggaagattt cccaggtgag atcctccgca atgccgccga ctttctccaa ggcttggtag
     6001 ataggcacgg tgccgatggg cactggcgag ttgcggataa tccactcgcg ggtttcatgg
     6061 atgtttttac cggtggagag atccatgatg gtatcggcgc cccagcggat gccccaggtg
     6121 agcttggcca cttcctcttc gatggaagag gtcaccaccg aattgccgat attgccgttg
     6181 attttcacca ggaaattacg gccaataatc atcggctcca attccacatg gttgatattg
     6241 gccgggataa tagcgcggcc cagggccact tcctggcgta caaactcagg ggtaatttct
     6301 ttggggatat tggcgccgaa actcatgccg gcgtgttgct gggataacac gccctgctct
     6361 ttggcttgtt gcaggctcat gttttcacgg atggcgatgt actccatctc gggggtgatg
     6421 atgccctttt tggcgtagtg catctggctg acattcatgc ccgccttggc ttggcgcgga
     6481 gcgcgcaaat gggcgaagcg cagcttttgc agggccgggt catctaaacg ctggcgagtg
     6541 aagtcggagg cggaacctgt cagcacctct gtatcggcgc gatctaaaat ccactggctg
     6601 cgaatatccg gcagcccttg gcgtaaatcg ataatgacat tggggtcgct ataggggcct
     6661 gaggtgtcgt acacccgcac cggcgggttt tgctcgccgc ccatatcggt gggggtatca
     6721 aacaggctga tctcccgcat gggcacgcga atatcatccc tactgccggt cacgtaaatt
     6781 ttttgcgagc gggggaaggg ttttaccgag gcttcgtcca ccttggcgtt ttggctgaca
     6841 aattgggtat caggtgtata ggacatattg gctaccaata ggcgtaaaaa tgatggattg
     6901 gcccattgct gggggccagt tgtaaacggg cgctctgttc taaagcgccc gcgataaacg
     6961 ttttagcggc ggcgatggct tggcgcaggt ttaagccctg ggcgaggccc gctgctatgg
     7021 cactggccag ggtgcagccg ctgccgtggc tgtgctcggc agcc
//
LOCUS       DAOQGS010000174         7039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5208_length_7039_cov_4.037085, whole genome shotgun sequence.
ACCESSION   DAOQGS010000174 DAOQGS010000000
VERSION     DAOQGS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_7039_cov_4.037085"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PKZ52_08175"
     CDS             <1..681
                     /locus_tag="PKZ52_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HNG59788.1"
                     /translation="QLSADDFSEIIIRLLDYGVLCRDESQIEQKLYDRFVQVEALVED
                     YLSVLAIRLQHDSRFAFVRAYPPGASAPDMQDDEHTPFNGGLRARLNQMEVALILVCR
                     IEYAKQLREARVDEHGCVLVSMEALSIAFTNLLKRSLPENITERRALFRRLRQLRLLS
                     YQQEDDLENPESYLRIRPAITSMVNDDALAVLANEEELAQLDAPADETESAEVAAVSI
                     RDQSSLFN"
     gene            754..4383
                     /locus_tag="PKZ52_08180"
     CDS             754..4383
                     /locus_tag="PKZ52_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNG59789.1"
                     /translation="MFLKRLILVNWGNIPKIELEFGPVNLFSGGNGSGKTTAADAIQA
                     LMTAAHENLFAFNPGQDETTQKGRGGKQVRTLASYLMGCDDGSFARLEDTDGYVAGVF
                     HPTQGEPSEAFTAVMAMRGHVEKSGQVKQAKLDDVFFLVLPGEELSLGHFIREAGSER
                     HLTPITKIQAEFKKFAAVEYYDKKGAYLRRLYGALRGRKDSVPDREAKHAARTFAGFM
                     AYKPVKSIHEFVAQEILEPRDLGEAIRSVSDLMKTIGSMEANAAMIKASIDTLDDARS
                     CVHRYVQLWLDQQTLNYTEAARQAWVNQQQYIAAKNIQAANRQAISECQQRSEIAAER
                     KQHLMSRMVQLQAQRQGVQSLRDKDAITSRINLLQKKLTDAAVPLQAQIQQLKNNLSA
                     GQDLLTYLDESSIGQEIPALVKANFIQQCKTICQNKNAQEVDFHRLINRDWVGVQSME
                     TSLDQVLSVQSQQNLWVDSFDLKPANNAGNLREQVRHLLDLRTQTRDKLAADIRKLSH
                     EINLRSNQHLAYPYYVEAALRTLQEQLPAADARVLCDYVDVSEEQWQMAIEGYIGGGR
                     FAIIVEREYEAEAMRILRNMKGDKRNNARVIQGAKASKDADRVHLPKHSILEVMKFSH
                     ATAEAYIKASYANVLRVDSVEELRNTARGITAEGMASGGYSMFRCDLDDGELVFGQGA
                     RLRALKAKEKELEKIQIQYAQAEKQRQSAEQLCQIIERIRHLDIGQVMHHLMDAAREL
                     QSAEQQLQQLDSGDTHELDAQLGSCQAEFAEHEQQSRLFEQEMGRLLEKQKQDDKRLE
                     AISIEQERLNLQRDAMEQLVLQAASLYPRFNAEEILPVCEQQAQTAESETQFVDTLKT
                     QQDALVKTERHLFEAISNHNLTAAIADKIVYETGYSTPHSAEFFKHISQLGAVIDELH
                     NRLKNNILVEKHRQLVQLKDSFNTAFVSNLCHSIYQAINEGKRILDDLNKELQHHRFG
                     ADRETFYFDVEWVPEYQEYWQFFKTLINLPNLGEGSTLFDVELPPKFARTRDQLLSML
                     LDNDEVTAHRELARISDYRNYRRYEIYKHPENKQPIALSQYGTGSGGQLETPAYIIRS
                     AAVTSAFKFNDGKSHMRMVMVDEAFSKMDEGRSREVIRYLTESLGLQLLFIMPTSKSG
                     PFLDIISNQFVFTKCPTNEPVGELQTRVLVDRKVCNSEQIKSLWAHHRKTIRHQAMLD
                     FMEDL"
     gene            4610..4921
                     /locus_tag="PKZ52_08185"
     CDS             4610..4921
                     /locus_tag="PKZ52_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59790.1"
                     /translation="MVKYLLMIFLGGICLEAGAVDTTALNIERVQFNASSSAYFAYRS
                     AGWGVCNNTQFVQLQGAPENRQIFSSQVLAAHMAGKTVAFAGSCTNPNGYLDATYIIV
                     N"
     gene            complement(5300..>7039)
                     /locus_tag="PKZ52_08190"
     CDS             complement(5300..>7039)
                     /locus_tag="PKZ52_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG59791.1"
                     /translation="GATLTLAPPVFAEANTDAAKTNARPLIWQPCFTDENPRLSCAKF
                     QVPFDYQARKGSLAAGKTIDLALIKLSAADTSQKPLGSLFLNPGGPGGSGFDWVRTDG
                     QRLFSQNLLNRYDLIGFDPRGIHYSTAITCQVQADNTAPFYPSVDFPTNPAQIKERIS
                     VNKNLSALCAQNGGDILQHMSTADVARDLDLMRQAVGDPLLNFVGYSYGSYLGVTYAN
                     LFPERVGRVIVNGVLDPVQWSTGRGFSGWLVPMTQRIGSDKGSMATLNEFFRLCDLAG
                     PDNCHFAPNSAQRFNALANTIKKQPIQLATPDGFQFEVDTGLFINTVKTSLYSTWNWQ
                     NLADLMAFTEAGAPPAVLSAQYTQLFNEIPANNIDQVPFDNNPLGFFSVLCSDSNNPR
                     DAWLWPLAAAVADSENGYFGSSWSWNSSICATWPANQLNRYAGPFNLRTRNTVLIANT
                     LFDPATPYSGAQAVRRLLPNSRLVTVAGWGHTTTGLSSCADNIIETYLLSGAVPQEDT
                     TCSQEALPFNLTMDTDVFTGMPKITDAPAPIIRFPLLKTQLSEQEKTKRAALARAILR
                     EVLPHTRRNTKGY"
BASE COUNT         1823 a         1594 c         1825 g         1797 t
ORIGIN      
        1 caattatccg ccgatgattt ttctgaaatt attattcgcc tattggatta cggcgtgttg
       61 tgccgggatg aaagtcaaat agagcaaaaa ctctatgatc gttttgtgca agtggaagcc
      121 ttggtggagg actatttaag tgttttagcg attcgcctgc aacacgacag ccgctttgcg
      181 tttgtgcgcg cttatccgcc gggggcgagt gcgccggata tgcaagatga cgagcacacg
      241 ccgtttaatg gcggcctgcg cgcccgttta aatcaaatgg aagtggcgtt aattttggta
      301 tgccgtattg agtatgccaa acaattgcgg gaggcgcggg tggatgaaca cggctgtgtg
      361 ctggtgtcca tggaggcctt atccatcgcc tttactaatt tattaaaacg ctccctgccg
      421 gaaaatatta ccgagcggcg cgccttattt cgccgtttgc gtcagttgcg gttattaagt
      481 tatcagcagg aggatgattt agaaaatcct gaaagttacc tgcgcattcg cccagctatt
      541 accagcatgg tgaatgacga tgcgctcgcg gtactggcca atgaagagga gttggcgcaa
      601 ttggatgcac cggcggacga aactgaaagc gcagaggtgg ccgccgtttc tattcgggat
      661 caatccagtt tatttaatta agccgcttag cgcggctgca gccaaaacct gcgcagccgc
      721 cccacatttt atttaacgaa tcgacatacg cgcatgtttt taaaaagact cattctggtt
      781 aactggggca atattccaaa aatagaattg gaatttggcc cggtgaattt attctccggc
      841 ggtaacggct cgggcaaaac caccgcggcg gatgccattc aggcactcat gaccgctgcc
      901 cacgaaaatt tatttgcctt taaccccggc caagatgaaa ccacccaaaa aggccgtggc
      961 ggcaaacagg tgcgcaccct cgcttcctat ttaatgggct gtgacgatgg cagctttgca
     1021 cgccttgagg ataccgatgg ttatgtggcc ggtgtatttc accccaccca gggtgaacct
     1081 agcgaggctt ttacggcggt aatggctatg cgcggccacg tggaaaaatc cggccaggtg
     1141 aaacaggcaa aattagacga tgtgtttttt ttggtgttac ctggcgaaga gctttcacta
     1201 ggccatttta ttcgcgaagc cggtagcgag cggcatttaa caccgattac caaaattcag
     1261 gcggaattta aaaaattcgc cgccgtggaa tattacgata aaaaaggcgc ctatttgcgc
     1321 aggctgtatg gcgctttgcg cggccgtaaa gattccgtgc ccgaccgaga agccaaacac
     1381 gccgcccgca cctttgctgg ttttatggcc tataagccgg ttaaaagtat ccacgaattt
     1441 gtcgcccaag aaatattaga gccgcgggat ttgggtgagg caatccgcag tgtatcggac
     1501 ttgatgaaaa ccatcggcag catggaagcc aatgccgcta tgattaaagc ctccatcgat
     1561 accttagacg atgcgcgcag ttgtgtgcat cgctacgtgc agctgtggct ggaccagcaa
     1621 accctcaatt acaccgaggc cgctcgccaa gcctgggtga atcagcagca gtatatcgcc
     1681 gcgaaaaata ttcaagcggc caaccgccaa gcgattagtg aatgccagca gcgcagcgaa
     1741 attgccgctg agcgtaaaca acatttgatg tcccgcatgg tgcaattgca ggcccagcgc
     1801 caaggtgtac aaagcctgcg cgataaagac gccatcacca gccgcattaa tcttttacag
     1861 aaaaaactta ccgatgccgc cgtgcctttg caggcgcaaa ttcagcaatt aaaaaataat
     1921 ttaagcgccg ggcaggattt attaacctac ttggatgaaa gttcaatcgg gcaagaaatc
     1981 cccgctttgg tcaaagccaa ttttatccag cagtgcaaaa ctatctgcca aaataaaaat
     2041 gcccaagagg tagattttca ccgtttgatc aatcgcgact gggtgggcgt gcagtccatg
     2101 gaaaccagct tggatcaagt gttatcggtg caatcccagc aaaatctgtg ggtggatagt
     2161 tttgatctga agcctgccaa taatgctggc aacctgcgcg agcaggtgcg ccatttattg
     2221 gatctgcgca cccaaacccg cgataaactt gccgccgata ttcgcaaact cagccacgaa
     2281 attaatctgc gcagcaacca gcatttagct tatccctatt atgtggaagc ggccctgcgc
     2341 accttgcaag aacaattgcc cgcagccgat gcacgggtgt tgtgtgatta cgtggatgtc
     2401 agtgaagagc agtggcaaat ggccattgag ggttatattg gcggcggccg ttttgccatt
     2461 attgtggagc gcgaatacga ggcggaagcc atgcgtattt tgcgcaatat gaaaggcgat
     2521 aaacgcaata atgcgcgggt gattcaaggc gctaaagcca gtaaagatgc cgaccgagtg
     2581 catctgccta aacactctat tttagaggtg atgaaattct cccacgccac tgccgaggct
     2641 tatattaaag cctcatacgc caatgtgttg cgggtggatt cagttgagga gctgcgcaat
     2701 actgcccgcg gcattaccgc tgaaggcatg gccagcggcg gatacagcat gttccgctgt
     2761 gatttagacg atggcgaatt agtttttggc cagggcgcgc gcctgcgggc attaaaggca
     2821 aaagaaaaag aactggaaaa aattcaaatt caatatgcgc aggcggaaaa acaacggcag
     2881 agcgccgagc agttgtgtca aattatcgag cgcatccgcc acctggatat aggccaggtg
     2941 atgcaccatt taatggatgc cgcgcgggaa ttacaaagcg ccgaacaaca attgcagcaa
     3001 ttagacagcg gcgacaccca cgaattggac gcgcaactgg gcagttgcca ggcggagttt
     3061 gccgagcacg agcagcaatc gcgcctattt gagcaggaaa tgggccgtct gttggaaaaa
     3121 caaaaacagg atgataaacg cctggaggct atttccatag agcaggagcg tttaaatctg
     3181 cagcgcgatg ctatggagca attggtattg caggcggcaa gcctgtaccc gcgctttaat
     3241 gccgaagaaa tattgcccgt atgtgagcag caggcccaaa cggccgaatc ggaaactcag
     3301 tttgttgata ctttaaaaac tcagcaggac gctttggtaa aaactgagcg gcacctgttt
     3361 gaggcgattt ccaatcacaa tttgaccgcc gccattgccg ataaaatcgt ctacgaaacc
     3421 ggctatagca caccccattc ggcggagttt tttaaacata tcagccagct aggggctgtg
     3481 atagatgagc tacataaccg tttaaaaaat aatattttgg tggaaaaaca ccgccagctc
     3541 gtgcagttaa aagacagttt taataccgcg tttgtcagca acctctgcca ctctatttac
     3601 caagcgataa acgaaggcaa acggattttg gacgacttaa acaaagagct gcagcaccac
     3661 cgctttggtg ccgaccggga aaccttttat tttgatgtgg agtgggtgcc ggaatatcaa
     3721 gaatattggc aattctttaa aaccctaatt aatttgccca acctggggga aggctcaacc
     3781 ctatttgatg tggagctacc ccctaaattc gcccgcactc gggaccaatt gctgagtatg
     3841 ttgctcgata atgatgaggt gacagcccac cgggaattag cccgtattag tgattaccgc
     3901 aattaccgcc gctatgaaat ttataaacac ccggagaata aacagcctat agcacttagc
     3961 caatacggca ccggctccgg cggccagttg gaaactccgg cctatattat ccgctcggcg
     4021 gcggttactt cggcgtttaa atttaacgat ggtaaaagcc atatgcgtat ggtgatggtg
     4081 gatgaggcgt tttcaaaaat ggatgagggc cgctcccgcg aagtgatccg ttacttaacc
     4141 gagagccttg ggttacaatt attgtttatt atgcccacca gtaaatcagg gccgtttttg
     4201 gatattattt ctaatcaatt cgtgtttacc aaatgcccta ccaacgagcc ggtaggtgag
     4261 ctgcaaaccc gtgtattggt ggatcgcaag gtgtgtaact ccgaacaaat taaatccctc
     4321 tgggcccacc accgcaaaac cattcgccat caggctatgt tggattttat ggaggatttg
     4381 taaaaatatt ctattttatc aagtgttttt cggtgtgctg gattttaacg cggggaattc
     4441 acagttccaa agtttttccc gatttttata ttcagttgtt tggcctaaga atgcctgggg
     4501 gtctgtttgt ggtgttttgt tggtgtatag gtgcgctgct gacgcttttt tcttgttgga
     4561 ttttgtaatt tttgctggcg atgctttttg atttatgagg gcttgaaata tggttaaata
     4621 cttgctgatg atttttcttg gagggatctg tttagaggct ggtgcggttg ataccaccgc
     4681 tttaaatatt gaaagggtgc aatttaatgc tagctctagt gcttatttcg cataccgttc
     4741 agccggttgg ggggtctgta ataataccca gtttgtgcag ttgcaaggtg ccccggaaaa
     4801 taggcagata tttagctctc aagtgttggc tgcgcatatg gctggtaaaa cagtggcgtt
     4861 tgcaggtagt tgtacgaatc ctaatggtta tcttgatgct acttacataa tcgtaaactg
     4921 aataggtttt ggcattgggg tttctgaaaa ttgttgagtt gggaaagcaa gctgctaggc
     4981 actatctcgg ttttatgctc tttatttgaa gtggtgctgt gtggatgaat ggtgagcgat
     5041 taagtcagtt gcgagcaagt cgcagtgtgg acaggcggtt gctgattaaa ttgtgtgtga
     5101 atcgctctat gataggcccc gttattgcgt gattttttaa aaaatattgc tggcataatg
     5161 tatttatcag caatcatgcc aattgatcta ccgccatcgt aatggaactg ttgagggggg
     5221 caaaggttta ttgccgttgc attgaaaata ctaacgaccc ggctaaccta tgattagccg
     5281 gatttttacc tctcgtatat caatatcctt tggtgttacg ccgcgtatgc ggtaaaacct
     5341 ctcgcaaaat agcccgagcc agcgccgctc gtttggtttt ttcctgctcg cttaattggg
     5401 tttttaataa tgggaagcgg ataattggtg cgggagcatc tgttattttt ggcatacctg
     5461 taaaaacatc cgtatccata gtgagattaa atggcagggc ctcctgtgag caggtggtgt
     5521 cttcttgggg gactgcgccg cttaacagat aggtttcgat aatattatct gcgcaggagg
     5581 ataagcccgt ggtggtgtgc ccccaacccg ctaccgtcac caaacgagaa ttgggtaata
     5641 agcgccgcac cgcttgcgca ccgctgtatg gcgttgccgg gtcaaataag gtattcgcaa
     5701 ttaatactgt attgcgagtg cgcaggttaa atggccccgc gtagcgattt aattggttgg
     5761 caggccaagt ggcgcagatg gatgaattcc aactccagct ggaaccaaaa tagccatttt
     5821 cactatcggc tacggcagca gccagaggcc aaagccaggc gtcgcgggga ttatttgagt
     5881 cgctgcacag gacagaaaaa aatccgaggg gattattgtc aaacggcact tgatcaatgt
     5941 tgttcgcggg aatctcatta aatagctgag tgtattgggc acttaatact gcaggtggcg
     6001 caccggcttc agtaaatgcc attaaatcgg caaggttttg ccaattccag gtgctgtata
     6061 aactggtttt gacggtatta ataaataaac ctgtatccac ttcaaattgg aaaccatcgg
     6121 gcgttgcaag ttgaatcggt tgttttttaa tagtgttggc tagagcatta aagcgttgcg
     6181 ctgaattagg agcgaagtgg caattatcgg ggcctgctaa atcacacaag cggaaaaatt
     6241 cattcagcgt tgccatactg cctttatcgc tgccaatccg ctgggtcatg ggcaccagcc
     6301 aaccgctaaa acctctgcct gtagaccatt gcacagggtc taacacacca ttcacaataa
     6361 ctcggcccac tcgctcggga aataaattgg cataggtgac acctaaataa gaaccgtagg
     6421 aatagccgac aaaattcaac aggggatcac ccacggcctg ccgcattagg tctaaatccc
     6481 gtgccacatc agccgtgctc atgtgctgca gtatatcgcc gccattttgt gcacacagcg
     6541 cgcttaaatt tttattaaca ctaatgcgct ctttaatttg tgccggattg gtggggaagt
     6601 ccacgctggg ataaaagggc gcagtattgt ctgcttgcac ctggcaggtt atggctgtgc
     6661 tgtagtggat acccctagga tcaaagccaa ttaaatcata gcggttgagc agattttgag
     6721 aaaataaacg ctggccatcg gtgcgcaccc aatcaaaccc actgcctccc gggccgccag
     6781 ggttgagaaa taaactgccc aaaggttttt ggctggtgtc tgctgcggat aatttaatta
     6841 gtgcaagatc aatggttttg ccggcggcca ggctgccttt gcgcgcctga taatcaaatg
     6901 gcacttggaa tttagcgcag gataaacgag gattttcatc ggtgaaacag ggctgccaaa
     6961 tgagcgggcg agcgttggtt ttggcggcat ctgtatttgc ctcagcaaac acgggtggcg
     7021 ctaacgtgag tgttgcccc
//
LOCUS       DAOQGS010000175         6992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5257_length_6992_cov_4.798760, whole genome shotgun sequence.
ACCESSION   DAOQGS010000175 DAOQGS010000000
VERSION     DAOQGS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_6992_cov_4.798760"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKZ52_08195"
     CDS             <1..336
                     /locus_tag="PKZ52_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNG59792.1"
                     /translation="VREQHARLDVALLRGLGEHVRTLRQGAPSLALSGPLARVGAAIL
                     FIIPGTLTDILALLLLIPATQSRVSRRWGDSGFSGGFSPRPSQGGGDIIEGEWVEENR
                     QNPRIGPHQ"
     gene            503..793
                     /locus_tag="PKZ52_08200"
     CDS             503..793
                     /locus_tag="PKZ52_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNG59793.1"
                     /translation="MKIRPLHDRVVVRRKEEETKTAGGIVLPGSAKEKPNQGVIVAVG
                     TGKVLDNGDVRPVDVKVGDVVVFGKYAGSDTIEVDGEELVILSENDIKAVLV"
     gene            838..2481
                     /gene="groL"
                     /locus_tag="PKZ52_08205"
     CDS             838..2481
                     /gene="groL"
                     /locus_tag="PKZ52_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816860.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNG59794.1"
                     /translation="MAAKDVLFGDKARQKMLVGVNVLADAVKATLGPKGRNVVLDKSF
                     GAPTVTKDGVSVAKDIELKDKFENMGAQMLKEVASQASDEAGDGTTTATVLAQSIVNE
                     GLKAVAAGMNPMDLKRGIDKAVIAAVAHIKSIAQPCTDTKSIAQVGTISANSDASVGA
                     IIAEAMEKVGKEGVITVEEGSGLENQLDLVEGMQFDRGYLSPYFINNQESMNVELESP
                     FILLVDKKISNIRELLPVLEAVAKSGKPLVIVAEDVEGEALATLVVNNMRGIVKVSAC
                     KAHGFGDRRKAMLQDIAVLTGATVISEEIGLDLEGASLEHLGQAKRVTMSKENTTIVD
                     GNGDAESIKARVNQIRVQIEDTSSDYDREKLQERVAKLAGGVAVIKVGAATEVEMREK
                     KARVEDALHATRAAVEEGVVPGGGTALIRAVAAIADLRGDNEDQNAGITIARRAMEAP
                     LRQIVANAGEEGSVVADKVKASSAANYGYNAATGVYGDMLEMGILDPAKVTRSALQAA
                     GSIAGLMITTEAMIADAPSDKAPAPDHGHGGMGGMGGMM"
     gene            2698..6840
                     /locus_tag="PKZ52_08210"
     CDS             2698..6840
                     /locus_tag="PKZ52_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG59795.1"
                     /translation="MAMVLLMASAQLSVQAAPVPSFRHLLTDEANRLQDNISAVNAIA
                     QDQQGFIWIGGENGLARYDGHQFVFFHGGNPGSGALCGNYVLSMVVDYTGALWIGTQQ
                     GLNRYLPATEEFECFLNDPALSTSLADNYVRALAVDANNRLLVGTARGLDVFDSATAT
                     FAHVRTQPRSLPPIRSLEVDSRHRVWIGTADEGLFRLDEIPVDSRDAELRVEHYGAQR
                     LIHPGVSAIAEDRQGRLWVGTAAGISRIEADLSSITHYPPQKNANGAVSKARVQDIAV
                     DINGVIWVATDREGLFVFQEEENQFAAVGTQQDRNRSLSSSNTRIIFNDRDGDIWVGL
                     FPRGVNYFNRATENIRNFIHQPGQLNGLRTRAVESLFEDKAHLIWLGGYTGVSIFDPQ
                     QQSFRHLTSDDAPVEGVISLAQTLDGDMWIGTWSAGLYRYSQQTGRFKHYLTAPAATA
                     LGSNYIWHLLVDPQGRLWVGTEDGGLNLYDPVSDSFKRFVHKADNPNSISNNFVWNMA
                     VADAEHLWLATAAGLNYFNTTTFEVERFGHSAEQNYGIKSQFLVELMVHSNGDLWIGS
                     QDQGVFIFNPRTGSVKTIGTQEGLAAAYVSSLVEDDDHNVWAATVSGISLLAPDGTHI
                     KNYDLNNGLVGNGYNRDTGLKDHNGGIYIGGTEGLNYFKPGELIVNRRAPNILLTGFY
                     VREQNWLQAGNGAPLQLNFEQNSLKFVFSAMSFRASQNNLYQTRLIGLDSQWSAPIAL
                     NSVNYNNLAPGRYELQVRGANSDGIWSDQYLRIPFVIAPPPWRSTWAYCAYGALTLFV
                     LAMLILLHRRRMQLSNEQALNHKLNNLDKMKDAFLANTSQELRAPLNGIIGLAQSLLD
                     GSVNQLDAQSRHTLNVIVASGKRLAHLVDDLLDSAKLMGQQMELEKAPINLYSLVSHV
                     SSLLQPMAAQKNLVLLNQVAKSVPAVLADEARVQQVLMNLVTNAIKYTEKGRIEIIAQ
                     VSEREIAISVSDTGIGINLVDLKTIFQPFSQVADNNPHKSRGPGLGLSICQQLVELHG
                     GRLTVSSEVGQGSIFKFTLPRVEGGVVGKKVNISKMDETLPMLDTYIAEHKNTHHHAL
                     RKILIVDDDPVSRLVIAKMLSAEPFSLIEVASGEEALQYLQADAAIELILLDIMMPSL
                     SGFSVCQRIREQFSAAHLPVVLVTSKDMPDDIARGFATGASGYLIKPINKAHMLKLIH
                     QQLAWLDNSPLLADAGVEMYEIFRSLILTYAPGQTPIDRMAELLVALKQGFRLAKVLG
                     IWRLRQQYCEPLYSDPACPLKTIQAAHKVRKISQLVKALDQQRLLTPLNEGDLETLAE
                     YLPAQDIKEWVIVPLVYEGQSRGAIVIGAQEAWPKHDLTLFTEAKPFCVAALLPLLAG
                     EH"
BASE COUNT         1705 a         1706 c         1885 g         1696 t
ORIGIN      
        1 gtccgagagc agcacgcccg cctcgatgtt gcgctgctgc gcggcctcgg tgaacatgtg
       61 cgcactctgc gccaaggggc gccaagcctg gccttgagcg ggcccttagc ccgcgtgggg
      121 gcggccattc tgtttattat cccaggcacc cttaccgata tcctcgcgct attgctgctg
      181 atacccgcta ctcagtcccg cgtgtccagg cggtggggcg atagtggttt tagcggcggc
      241 tttagcccca ggccgagcca gggcggcgga gatattatcg agggcgagtg ggtggaggaa
      301 aaccgacaaa acccccgcat tggcccccac caataaaatt tttgttcacg ggtgttgaaa
      361 tcccttggtg tgcacccatc ttccgaccac accaaaaagc ctgcgggcat caagccacta
      421 cgctcgcccc agggtttgta atttttacta cttagctcag ctaagtaatt ccactgacca
      481 ttgttacttt ggagaaccat taatgaaaat ccgtccttta cacgatcgtg ttgtagtacg
      541 tcgcaaggaa gaagaaacca aaacggcagg cggcattgtg ttgcctggct ctgctaaaga
      601 gaaacccaat caaggtgtta tcgttgcggt aggtaccggt aaagttttgg ataacggcga
      661 tgtgcgtcct gtggacgtca aagtgggtga tgttgtggtg ttcggcaaat acgccggcag
      721 tgacaccatt gaggttgacg gcgaagagct ggtcatcctc agcgaaaacg acatcaaagc
      781 cgtacttgtt taatttctcg tttaataaca actcactgaa ttaggtagga aaatatcatg
      841 gcagctaaag acgtattgtt tggcgataaa gcccgtcaaa aaatgttggt cggtgtaaac
      901 gtattggccg acgctgtaaa agcaactctt ggccccaaag gccgcaacgt cgtattggat
      961 aaatccttcg gcgctccaac tgttaccaaa gacggtgtgt ctgtagcgaa agatatcgaa
     1021 ttaaaagaca aattcgaaaa catgggcgca cagatgctca aagaagtggc ctcccaagcc
     1081 tccgacgaag ccggtgacgg taccactact gccactgtac tggctcaatc cattgtgaac
     1141 gaaggcctta aagccgttgc tgcaggcatg aaccccatgg acctaaagcg cggtattgat
     1201 aaagccgtta tcgccgccgt tgcccacatc aaatccatcg cccagccttg caccgacacc
     1261 aagtctatcg ctcaggtagg tactatctct gctaactccg acgcttctgt gggtgccatc
     1321 atcgccgaag ctatggaaaa agtaggtaaa gaaggtgtga tcaccgttga agaaggttcc
     1381 ggccttgaga accagctgga cctggtagaa ggtatgcagt ttgaccgcgg ctacctgtct
     1441 ccttacttca tcaacaacca agagagcatg aatgttgagc tggaaagccc tttcatcctg
     1501 ttggtagaca aaaaaatctc caacatccgc gagttgttgc ccgtattaga agccgttgct
     1561 aagtctggta aaccgctggt aatcgtggct gaagacgtag aaggcgaagc cttagcgact
     1621 ttggttgtga acaacatgcg cggcatagtt aaagtttctg cctgtaaagc ccatggtttt
     1681 ggcgaccgcc gcaaagctat gttgcaagac atcgctgtat taaccggcgc caccgtaatc
     1741 tctgaagaaa tcggcctgga tttagaaggc gcatctttag agcatttagg ccaagccaag
     1801 cgcgtgacca tgtcgaaaga aaacaccacg attgtagacg gcaacggcga tgctgaaagc
     1861 atcaaagccc gcgttaacca aatccgcgtg caaatcgaag acaccagctc tgactacgac
     1921 cgtgaaaagc tgcaggagcg cgttgccaaa ctcgctggcg gcgtagcggt aattaaagta
     1981 ggcgcggcaa ctgaagtgga aatgcgcgag aaaaaagccc gcgtagaaga cgccctgcac
     2041 gcaacgcgcg ctgcggttga agaaggcgtg gtgcctggcg gtggtactgc cttgatccgt
     2101 gcggtagcag caattgccga cttgcgtggc gacaacgaag atcaaaatgc cggtatcacc
     2161 atcgcgcgcc gcgccatgga agccccgctg cgtcagatcg ttgccaacgc gggcgaagaa
     2221 ggctcggtag tggctgacaa ggttaaagcc tctagcgctg ctaactacgg ttataacgcc
     2281 gccaccggcg tgtacggcga tatgctggaa atgggtatct tagatcctgc caaagtaact
     2341 cgctctgcac tgcaagcagc gggctctatt gctggcctga tgatcaccac tgaagccatg
     2401 attgccgatg ccccatctga caaggctccg gctccagacc atggccacgg cggcatgggt
     2461 ggcatgggcg gcatgatgta aaccgcgctt agccaagcta agccttaaaa aaccgctgga
     2521 gcaatccagc ggtttttttt cgcccctcta aaacccgccc ggtttgtacg ggggcaagtg
     2581 gagtgaataa cccccgtcta tactcgttaa agcttggtgc gcctgtgcac cgggtggtag
     2641 gggcttttgc ctgggaatct ataaagagag aaatccttgt tgtcactgct gcttcgaatg
     2701 gcgatggtcc tactgatggc gagtgcgcag cttagcgtac aggccgctcc ggtgccgtca
     2761 tttcgccatt tgcttacaga tgaagccaac cgcttgcaag ataacatcag tgcggtaaac
     2821 gctattgccc aagaccaaca ggggtttatt tggatcggtg gtgaaaatgg tttggcccgt
     2881 tacgacgggc accagtttgt ctttttccac ggtggcaacc caggctccgg cgccctgtgt
     2941 ggcaactatg tcctatccat ggtggtggac tacacgggtg ccctttggat cggcacccaa
     3001 caggggctca atcgttacct gccagccact gaagagtttg agtgtttcct caacgacccc
     3061 gctttaagta cctccctggc cgataactat gtgcgcgcct tggcggtgga cgccaataat
     3121 cgcctcttag tgggcaccgc ccgcggcctg gatgtttttg attcggccac cgccaccttt
     3181 gcccatgtgc gcacccagcc taggtcgctg ccacccattc gcagccttga agtggatagc
     3241 cgccaccggg tgtggatcgg cactgccgat gagggtttgt ttcgcttgga cgaaattcca
     3301 gtggatagcc gcgacgctga gctgagggtt gaacactatg gcgcccagcg gctaattcac
     3361 cccggcgtaa gtgccatcgc agaggatcgc cagggccgct tatgggtggg gaccgccgca
     3421 ggtattagcc gcatcgaggc ggatctctcc tccatcaccc attatccgcc gcaaaaaaat
     3481 gccaatggtg cggtatccaa agcccgcgtg caggatatag cggtggatat aaacggcgtg
     3541 atttgggtgg ccaccgatcg cgagggttta tttgtttttc aggaggagga aaatcaattc
     3601 gcagccgtgg gtacccagca ggatcgcaac cgcagcctca gcagcagcaa cacccgtatt
     3661 atttttaatg atcgagacgg tgatatttgg gtgggtttat ttccgcgcgg ggttaattat
     3721 tttaatcgcg ccacggaaaa tatccgcaat tttattcacc aacccggcca attaaacggt
     3781 ttgcgcaccc gcgccgtgga gagtttattt gaggacaagg cgcatttaat ttggctgggg
     3841 gggtataccg gcgtcagtat ttttgatccc caacagcaga gctttcgcca tttaaccagc
     3901 gatgatgcgc cggttgaagg cgtgatttca ctcgcccaaa ccctcgatgg cgatatgtgg
     3961 attggcacct ggtcggcagg tttgtaccgt tattcacagc aaacggggcg ctttaaacac
     4021 tatttaacag cgcctgcggc cactgcttta ggcagtaatt atatctggca tttattagtg
     4081 gacccccagg gcaggctgtg ggtgggcacc gaagacggcg gattaaattt atatgacccg
     4141 gttagcgata gctttaagcg ttttgttcac aaggcggata atcctaacag catcagcaat
     4201 aattttgtgt ggaatatggc ggtggcggat gccgagcatt tatggttggc caccgctgct
     4261 gggttgaatt atttcaatac caccacgttt gaagtggagc gctttgggca tagtgcggag
     4321 caaaactacg gcataaaaag ccagttctta gttgaattaa tggtgcactc caatggcgat
     4381 ttgtggatag gcagccagga tcagggcgtt tttattttta atccgcgcac cggcagcgta
     4441 aaaaccattg gaacccagga gggattagcg gcggcctatg tatcaagctt ggttgaggat
     4501 gacgatcaca atgtgtgggc ggcaacggtc agtggaattt ctctgcttgc gccggacggc
     4561 acccatatta aaaattatga tttaaacaac ggtttggtag gcaatggtta caaccgtgat
     4621 acggggttaa aagatcataa cggcggtatt tatattggtg gcactgaagg attaaattat
     4681 ttcaaaccgg gggagttaat tgttaaccgc cgcgcgccca atatattatt aaccggcttt
     4741 tatgtgcgtg agcaaaattg gttacaagcc ggtaatggcg cgcctttgca gctgaacttt
     4801 gagcaaaact cgcttaaatt tgtcttctca gccatgagtt ttcgcgccag ccaaaataat
     4861 ctgtaccaaa cccgtttgat aggccttgat tcacagtgga gtgcacccat tgcactcaat
     4921 agtgtcaatt acaacaattt ggcacctgga cgttatgaat tacaggtgcg cggggctaat
     4981 tctgatggta tttggagcga tcaatacctg cgtattccct ttgtcatagc gccgccgccc
     5041 tggcgctcca cttgggcata ttgcgcctat ggcgccctga cgctttttgt gctggccatg
     5101 ttgatcctgt tgcaccgcag gcgcatgcag ttgagcaatg agcaagcgct gaatcataaa
     5161 ttaaataacc tggataaaat gaaagatgct tttttggcga atacctcgca ggaattgcgc
     5221 gcgccgttaa atggcattat tggccttgcc caatcgctac tggacggctc ggtaaatcag
     5281 ctggatgccc aaagccggca tacgttgaat gtgattgtgg ccagtggcaa gcggctggcc
     5341 catttggtgg atgatttact cgacagtgcc aaattaatgg gccaacaaat ggaattggaa
     5401 aaagcgccga ttaatctata ttccctggtc tcccacgtca gcagcttgct acagcctatg
     5461 gccgcgcaga aaaatcttgt gttgctcaac caggttgcca aaagtgtccc cgcagttttg
     5521 gctgacgaag cccgcgtgca gcaggtgttg atgaacctgg tgaccaatgc tatcaaatat
     5581 accgaaaaag gccgcattga aattattgcc caagtctccg agcgcgagat tgcgattagt
     5641 gtcagtgata caggtatcgg aattaatttg gtggatttaa aaacaatttt tcagcccttt
     5701 agccaggtgg cggataataa tccccacaag agccgtgggc cgggtttggg tttatcaatt
     5761 tgccaacagt tggtggaatt acacggtggc cgtttgacgg tatcatcaga agtagggcag
     5821 ggttcaattt ttaaattcac cttgcctaga gttgaaggcg gtgtggtggg taaaaaagtg
     5881 aatattagta agatggatga aaccctgccg atgctggata cctatatcgc cgagcacaaa
     5941 aatacccacc atcatgcgct gcgcaaaata ctgatcgtcg atgatgaccc tgtcagccgc
     6001 ttagtcattg cgaaaatgtt atcggctgag ccatttagcc tgattgaggt ggccagcggt
     6061 gaggaagctc tgcagtattt gcaggcggat gctgccattg agttaatttt attagatatt
     6121 atgatgccaa gcttatccgg gtttagcgtc tgccagcgca ttcgtgaaca atttagcgcg
     6181 gcacatttgc ctgttgtttt agtgacgtcc aaagatatgc cggatgatat agcccggggc
     6241 tttgccaccg gtgccagtgg gtaccttatt aaacctatta ataaagccca tatgcttaag
     6301 ctgattcacc agcaattagc ttggctggat aacagcccac tgcttgcaga cgcaggggtg
     6361 gaaatgtacg aaatttttcg ctcccttatt ctcacctatg cacctgggca aacgccgatt
     6421 gatcgcatgg cagaattatt ggtggccctt aaacaaggct ttagattggc gaaagtgctg
     6481 ggcatttggc ggttgcgtca gcagtactgt gaacccctgt atagcgatcc ggcctgcccg
     6541 ttaaaaacta ttcaggcggc gcacaaagtg cgcaaaatta gccagctggt caaagccttg
     6601 gatcagcagc gtttgttgac gccactaaat gaaggcgatt tggaaaccct ggcggagtat
     6661 ttacctgccc aggatataaa agagtgggtc attgtgcctt tggtgtacga aggccaaagt
     6721 cgcggtgcca ttgtaatcgg cgcgcaggaa gcctggccga aacacgattt aactctattt
     6781 accgaggcta aacctttttg tgttgcggcg ctgttaccgc tgctggctgg ggaacactaa
     6841 gagtgttaat ttcagagttc agcctttatt actatctttg cgcaagcaat gaatacatat
     6901 catttcagac cgtcaaaaag agggatcttt ttgacgggcc tacaaggttg tattcacggc
     6961 gtgtctgaaa tgatgtgtat ttattgccgg tg
//
LOCUS       DAOQGS010000176         6977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5271_length_6977_cov_3.789367, whole genome shotgun sequence.
ACCESSION   DAOQGS010000176 DAOQGS010000000
VERSION     DAOQGS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5271_length_6977_cov_3.789367"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..947)
                     /locus_tag="PKZ52_08215"
     CDS             complement(396..947)
                     /locus_tag="PKZ52_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNG59796.1"
                     /translation="MKVGHGHRSPQGVFYGMYYCIPWNVMSSMPMKKPPHILGAGVQA
                     LAIVYQQLAARLNRQLEPMGLNMTQISLLTHLVNVKREETVLSLAQVMQMNQPAVTKA
                     IQAMEGRGWIAKNKSATDARVSHLMVTPAGHEHLHHAQQACLPLLAQTFVGLELEELN
                     QLIILLQKINKTAWKTEQQKGSA"
     gene            927..1559
                     /locus_tag="PKZ52_08220"
     CDS             927..1559
                     /locus_tag="PKZ52_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59797.1"
                     /translation="MTMTNFHSRFIRHSLNSQYALWRWWLLAITGLVVARLVQMFWLD
                     PAYAQSQYPVPFFVGQTTFNAEELKGYYQVMLDKGTLPLYWLTQCIDFVFIACTYWAF
                     FALTQSIYHSIKRVLPQASRWQVVAKVWCFIAPLAAVADAAENIVSFIMLLQPVTFLD
                     FWVYPYSSFAVIKFAIFALTYLWAVINLFALLVILAVHRLQHLFTKPILN"
     gene            1774..3030
                     /locus_tag="PKZ52_08225"
     CDS             1774..3030
                     /locus_tag="PKZ52_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNG59798.1"
                     /translation="MLQTISLGIFAFIIATTLLISYKPLRQVGKWLGISVGGVAVLAA
                     AVYGYFMRPIHIPAAPVVDAQTALSNTKELLAKANFYLGAGVYTAEDINHPAFAGFFN
                     SYSSINELKIGSVLKSPHERSYDFTQADQFITQAQQQDLRIRGHALVFGKLSDVYERP
                     NLQTWLDTHYSDATQKRHALQELVDHHIDKMLSHYKGRITQWDVVNEPLGLFGQGSLE
                     DNVFLRTLGPDYIARAFTRAHAVDGDAKLFLNEQFDRYTGPRAEAFINLVRALKDKGV
                     PLHGVGLQHHMLFTLAAAQETKEFIERLAALGVEVEITELDARLRLFADEQNPHLAQA
                     EYYRDIIKTCFDISACKGVTFWGLHDQDAWHKEIPWMFPNPNAPYLFDENKTAKIAVQ
                     LISEEIARWQPRGNRENPEVKEPLIN"
     gene            complement(3171..5564)
                     /locus_tag="PKZ52_08230"
     CDS             complement(3171..5564)
                     /locus_tag="PKZ52_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNG59799.1"
                     /translation="MRNQTKWCWLLAPALLMGAAASSAATYSASATTGGPGTAGCTGS
                     KACTDPGWGIDLNDNTGYRWSIPGGTGGPAQLFIKAASLSGERRMSLWLNNTRINTIT
                     LNSTNAPRPQGKEFGAYAVTLLAGTNNVELRDTEGTAEFDAIALRVEATSVGRSINNR
                     LEAESNDGMAGVQFEPSADQDGTQSAGWVDAGDYIEWQINVPTAGYYQLTSRSAAISD
                     AALDVWVNGTYTSELGIDKTQGWQAWKDFNSSSFYLAAGSQKLRVRFASAGQNLNWVK
                     LNPTVRTSEDVFSPGAWRMVSREDRSSLGLNNLGALSGSDYYGLDSQLWYLAPRGGGR
                     YEFKNKTSNCFSSQDNQSLTSTRIASCNTSTSAWGLELMRARSESRPAIYRLRAPNGL
                     CARPNGINAPILGACDLGSRWYLEASGWGERTGNREYTLNGLLLIKPTTDVPGLTFAQ
                     IPQDIVDAAKVSFVTGVKTWLERITDGRVSFIGATQVSTDPINQLYLEGGNWLPADWT
                     LPNDVQRFVPRGAYDTVQVFYTAGAVPDGWGWGPGRSIASNHTLWTTVSGGDVPASAW
                     TSGHNEPTEVFIHEPMHGLDGHYEALGVPLPEGLLHGAGVNRYGWYLNIEYNGWVHFY
                     RDYWLGQIIATDDTYRGYGPRMFRLPTVRQWALTQSASSAKIMQFSSGKCWVPQGGVA
                     QPSAGTRLVLTGQCFSAATSFVLEANGMLRHKSSGMCVYPRGGSAVNNAELILQPSCS
                     LNTNATLAITDLGSLRNNQTLRCVHPLGGSDQPAENTPVIFHDGCDEGRLRFHFQQE"
     gene            complement(6420..6605)
                     /locus_tag="PKZ52_08235"
     CDS             complement(6420..6605)
                     /locus_tag="PKZ52_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59800.1"
                     /translation="MHLKSVTLILFTALGFISAKTRADTVFAVFRVQVFDRDHQVLAD
                     STDFTQFAVFNQVWASK"
     gene            complement(6596..>6977)
                     /locus_tag="PKZ52_08240"
     CDS             complement(6596..>6977)
                     /locus_tag="PKZ52_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59801.1"
                     /translation="ATGQAAVFALDKPLAAATSAQALNHQLLDRVRFAEDYQVLASQL
                     TGSGVATNFIERLFLLVLKAKPQAELLELAEAGFTIMQTLGRNMIKDGKKLESAEDNI
                     GALAEQFALLQQGKVEVWRQLGCI"
BASE COUNT         1838 a         1755 c         1596 g         1788 t
ORIGIN      
        1 acgattcgcg gagtagcagt ggtgactaca ggtgaatgca gcccctcaaa aagagggacc
       61 tttttgacgg cctacacgga tgtattcacg gcgtgtctgc actaacctgt ggtcaccacc
      121 gcgggcgccg tattgagggt ttacagcact aaaacacgca cgagatagtt cctagtcggt
      181 gccggaattg cctccagcca cgtaaaaagt ggctaaatag ttacaaataa ttttgcttta
      241 acccatcaaa accagtgtaa aagcgcatac atgtaagcag ccttaaggaa gttcaattta
      301 atcagcgcat cctttagccc ccctcctctc actgccatat ctcctatagc agataacacc
      361 gtttgcgctg atttgtatat ttgatggatc gctgattaag cagatccttt ttgttgttcg
      421 gtcttccagg ctgttttatt tattttttgc aacaaaataa ttagctgatt taactcttcc
      481 aactccaacc ctacaaaagt ttgtgccagt aaaggcaaac aggcctgttg ggcgtgatgt
      541 aaatgctcgt gccccgccgg ggtaaccatt aaatgactca cccgagcatc tgtagcgctt
      601 ttattttttg cgatccaccc ccgcccttcc atggcttgaa tggctttggt gacggcgggc
      661 tggttcattt gcatgacctg ggccaggctc aaaacagttt cctcccgctt cacattcaca
      721 aggtgagtca aaagtgaaat ctgcgtcata ttgagcccca tgggctcaag ttggcgatta
      781 agcctggccg ctagctgctg atagacgata gcgagtgctt gcacaccagc gccgagtata
      841 tggggaggtt ttttcatagg catgcttgac attacattcc acggaataca ataatacatt
      901 ccatagaata caccttgcgg agacctatga ccatgaccaa ctttcactct cgatttattc
      961 gccacagcct caatagccaa tacgccctat ggcgctggtg gttgctcgca atcacaggcc
     1021 ttgtggttgc ccgtttagtg cagatgtttt ggttagatcc agcctatgct cagtcccagt
     1081 acccggtacc atttttcgtc ggccaaacca cctttaacgc cgaagaactg aagggttatt
     1141 accaggtgat gttggataaa ggcaccctgc ccctgtattg gctcacccaa tgtattgatt
     1201 ttgtgtttat cgcctgtacc tattgggctt tttttgccct gacgcagagt atttatcaca
     1261 gtattaaacg cgtgttaccc caggcatcgc gctggcaggt ggtggcaaaa gtatggtgtt
     1321 ttattgcacc tctagcagcg gtggcggatg cggccgaaaa catagtgtcg tttattatgc
     1381 tgctgcaacc tgtaaccttt ttggattttt gggtgtatcc ctacagtagc tttgctgtta
     1441 ttaagtttgc tatttttgct ctcacctatt tatgggctgt aattaatcta tttgcactgc
     1501 tggtgatctt agctgtgcac agattacagc atctatttac aaaacccatc ctgaattagc
     1561 atttttggca accataggta tatcgatatc ttcagctaca aagatatgcc ttgtgcgttt
     1621 gcacggtatt gaatatgaat taacaaggtt tttagctgct ccatacacaa caacattact
     1681 aaacaagctc aaacacactc tataacacct ggctagggga ataaataccg tcattccctc
     1741 caatgactac aaccaactaa gtgatcagct attatgttac aaaccataag tcttgggatt
     1801 tttgctttta ttattgctac cacactgtta attagctaca aacctttgcg ccaagtaggc
     1861 aaatggctgg ggattagtgt cggcggggta gcagtactcg ccgccgcagt ctacggctat
     1921 tttatgcgcc ccattcatat acccgccgcc ccagtggtag atgcccaaac ggcgcttagt
     1981 aatactaaag agctgctcgc taaggccaat ttttatttgg gcgcgggtgt ttatactgcc
     2041 gaggatatta accacccagc ctttgccggt ttttttaatt cctacagcag cattaatgaa
     2101 ttaaaaatag gctcggtgct aaaaagcccc catgagcgct cctatgattt tacgcaagcg
     2161 gatcaattta taacccaagc acaacagcaa gatttgcgta tcaggggcca cgccttggta
     2221 tttggcaaac tcagtgatgt gtatgagcgg cccaatttac aaacctggtt ggacacccat
     2281 tacagcgatg cgacacaaaa gcgccatgcg ttacaggagt tggtggatca ccacatagat
     2341 aaaatgctca gccattataa agggcgcatt acccagtggg atgtggtgaa tgaacctctg
     2401 ggtctgtttg gccagggcag cctggaagac aatgtatttt tacgcaccct aggccccgac
     2461 tatatcgccc gtgcatttac ccgcgcacat gcggtggatg gcgatgctaa attattctta
     2521 aacgaacaat tcgaccgcta cacaggcccc cgcgcagagg catttattaa cctggtgcga
     2581 gcattaaaag acaaaggtgt gcccctgcac ggcgtaggtt tgcagcacca tatgttattt
     2641 accttggccg ccgcgcagga gaccaaagaa tttattgaac gcctcgccgc attgggtgta
     2701 gaggttgaaa ttaccgaact ggatgcacgc ttgcgtttat ttgccgatga acaaaatccg
     2761 cacctggcac aggcggaata ttatcgggat attattaaaa cctgttttga catcagcgcc
     2821 tgtaagggtg ttaccttttg gggcctacac gatcaagatg cctggcataa agaaataccc
     2881 tggatgttcc caaacccgaa tgcgccctat ctatttgacg agaataaaac tgccaaaatt
     2941 gccgtgcagc ttatcagtga ggaaattgcc cgctggcagc ccagagggaa tcgcgaaaat
     3001 cctgaagtta aagaacctct gattaattag gattttcaga cgtggcacca tggtaagggc
     3061 cttccgccag gtgcggcggt gaaaataaga agtaatcagt gcagggaaaa acagacctac
     3121 ttaaacctaa acaataccta ataactcgcc atccctggcg agttttttaa ttactcttgc
     3181 tgaaaatgaa agcgtaagcg cccttcatca cagccatcgt gaaaaatcac tggagtgttt
     3241 tcagcgggtt gatccgaacc gcccagtgga tgcacgcaac gcaaggtttg gttgttgcgc
     3301 agactgccta agtccgtaat cgcaagtgtt gcattagtat ttaggctgca ggatggctgc
     3361 aaaattaatt cggcattatt aacagcactg ccgccccttg ggtacacaca catgcccgag
     3421 gatttatggc gcagcatgcc attggcctcc aacacaaatg atgtggcggc ggaaaaacac
     3481 tgaccggtta aaacaaggcg cgtacccgca ctcggttggg ccacaccgcc ctgtggcacc
     3541 cagcatttgc cactgctaaa ttgcataatt ttggccgaag acgcagactg ggtgagtgcc
     3601 cattgccgca ccgtgggcaa cctgaacata cgcgggccgt aacctctgta ggtgtcgtca
     3661 gtggcaatta tttggccgag ccaataatcc cgataaaaat gcacccagcc gttatattca
     3721 atatttaaat accaaccgta tcggtttaca cccgcgccgt gtaataagcc ctccggcaat
     3781 ggcacgccta aggcttcgta atggccatcc aacccgtgca tgggttcatg gataaaaact
     3841 tccgtgggtt cattgtggcc agaagtccaa gcagatgcgg gtacatcgcc accggaaaca
     3901 gtcgtccaga gtgtatggtt agaagcaata ctgcggccag gcccccagcc ccaaccgtcg
     3961 ggaaccgcac ctgccgtgta aaacacttgt accgtatcgt aggcgccgcg gggcacaaag
     4021 cgctgcacat cattgggtaa ggtccaatcg gcgggcagcc aattaccacc ttctaaataa
     4081 agctgattga ttggatccgt ggatacttgc gtagcaccta taaaactgac gcgaccatcg
     4141 gtaatccgct ccagccaggt ttttacgcca gtcacaaacg atactttggc ggcatcaact
     4201 atatcctggg gaatttgtgc aaaggttaaa ccgggcacat ctgtggtggg cttgatgagt
     4261 aataaaccgt tgagggtgta ttcgcggttg ccggtgcgct cgccccagcc ggatgcttct
     4321 aaataccaac gggaacctaa atcacaggcg ccaagtatgg gggcgttaat gccattggga
     4381 cgtgcgcata aaccattagg cgcgcgtaaa cggtaaatgg ccgggcgcga ttctgagcga
     4441 gcgcgcatta attctaaccc ccaagcagag gtagatgtat tacagctggc gatacgggtt
     4501 gaggttaggg attgattgtc ctggctgcta aaacaattac tggttttatt tttaaattca
     4561 tagcggccgc ctccccgcgg cgccaaatac caaagctggc tatctaaccc gtaataatca
     4621 ctgccactta aggcacccag attatttaag cctaggctgc tgcgatcctc ccgcgaaacc
     4681 atccgccagg cgccggggct aaaaacgtct tcggatgtgc ggacagtggg atttaattta
     4741 acccaattca gattttgccc ggcacttgca aaccgcacgc gcaacttttg gctgcctgca
     4801 gctaaataaa aactgctgga attaaaatct ttccacgcct gccagccctg ggttttatca
     4861 attcctaatt cgctggtgta ggtgccattt acccacacat ccaaagccgc atcactaata
     4921 gctgcgctgc ggctggttaa ttgataataa cctgccgtgg gcacattaat ttgccactca
     4981 atataatcac ccgcatccac ccagccggcg ctttgtgtac cgtcttgatc ggcagacggc
     5041 tcaaattgta cacccgccat accatcgttc gattctgcct ctaaacgatt attaatagaa
     5101 cggccaacgg aggtagcctc cacccgtaag gcaatagcat caaattccgc tgtaccttct
     5161 gtatcgcgca attccacatt attggtgcct gctaaaagcg taactgcata ggcgccaaat
     5221 tctttaccct gcgggcgcgg tgcattggtg gaatttaatg taattgtatt aatgcgcgta
     5281 ttattaagcc acaggctcat tctgcgctca ccgctcaacg aggccgcttt aataaataat
     5341 tgtgcaggcc cacccgtgcc gccggggatg ctccatcggt aaccggtatt atcgtttaaa
     5401 tcaatgcccc aaccgggatc ggtacaggcc ttgctaccgg tacaaccggc cgttcctggc
     5461 ccgccggttg tggcactggc cgaataagta gccgctgaac tggcagccgc ccccatcaga
     5521 agggctggcg caagcagcca gcaccatttt gtttgattcc gcatatacac tccgcgtatt
     5581 acttaagtaa tgtgtttatc ctaaaagcgc tttaaatccg ctacccaatc aaccaaaccg
     5641 ccgatgcaag ttcaatgatt attaggattg tggttagcaa atgtgtatta actatttgct
     5701 agggggagcc taccgtctgg aggtaacgaa atcaaaagac aaaaatgcat acatcatatg
     5761 cacaaatgaa tagctggata aaacaaaata accctaaaat acacgcacca tttaatttga
     5821 gcatgataag gttaatgttt gtgtgggaaa caagctattc cctaacttaa ggacattact
     5881 tttaaaaaaa aatcttcgta gctattcaga acaattttgt tgtagctgga cgtgattctc
     5941 gtaccaaaga gaaatctcca ggcgactgcg taagtgttgt gagccctaag tcactccttc
     6001 gcggcgattc tgccgcatgg ctaaatctgg cttaatttgc cgcccgcgct taagagggta
     6061 ataaggtgtg ctgagtggtt acaaatctct aaacaaggtg tttgacgggt gctccaggtg
     6121 attttgcgcc ctaagctagg attgattttt gcgcagactg aaaatgatcg ggcgagtgct
     6181 ttaatacctg caaactcttt aaactcttcc ctacaggggg tgggaaaaaa taagcgctga
     6241 taagtaccga atagttacaa cactccttgc cagcgcgtcg tcgcctacgt atttcaacgg
     6301 gctgctaggt gatccaattt agtgatgtaa ccatccctag cgtaaagcca tacacccata
     6361 ccggctaaaa taatcgaata ctgaaattca ctacgcgctt tataccggcc tggcacagcc
     6421 tatttactcg cccacacctg attaaagaca gcaaactgtg taaagtctgt actatcggcc
     6481 agcacttgat gatcgcgatc aaatacctgc acgcggaaaa ctgcaaagac agtatcggcc
     6541 cgggttttag ctgatataaa cccaagcgca gtaaacagaa ttagtgtcac agattttaaa
     6601 tgcatcctaa ctgccgccaa acctccactt taccctgctg cagtaacgca aactgctccg
     6661 ccagcgcccc tatattatct tcagcggatt caagtttttt accgtctttt atcatattgc
     6721 gccccagggt ctgcatgatg gtaaaacctg cctcggccag ttcaagtaat tcagcctgcg
     6781 gcttagcctt tagcactaat aaaaataaac gctcgataaa attggtggca acacccgagc
     6841 cagttaactg cgaggccaat acctgatagt cctcggcaaa acgtactcga tccagcaatt
     6901 gatgatttaa tgcctgtgct gatgttgccg ctgcaagtgg tttatctaaa gcaaatacgg
     6961 cggcttggcc agtggca
//
LOCUS       DAOQGS010000177         6861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5400_length_6861_cov_5.183808, whole genome shotgun sequence.
ACCESSION   DAOQGS010000177 DAOQGS010000000
VERSION     DAOQGS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5400_length_6861_cov_5.183808"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKZ52_08245"
     CDS             <1..363
                     /locus_tag="PKZ52_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNG59802.1"
                     /translation="CIMTQYFCQVEHLFDVGPECFRPVPKVESAIVRLTPHRQLPFQA
                     KSHEALEGVVRTAFSQRRKTLRNALKLVFNDQQLEDAGLNPNLRPENVALAQYVQLAN
                     TYTQIHGNSVLSAAGLPE"
     gene            396..770
                     /gene="apaG"
                     /locus_tag="PKZ52_08250"
     CDS             396..770
                     /gene="apaG"
                     /locus_tag="PKZ52_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNG59803.1"
                     /translation="MQTDDIQVSIKTQYLSDQSEPAQERFVFAYTITIANKSDQPAQL
                     ISRHWIITDGNNQVQEVQGIGVVGQQPRLDPGASYTYTSGVVLPTGMGTMTGTYQMRL
                     DNGDEFDAPIPTFALIHPQSLH"
     gene            770..1579
                     /locus_tag="PKZ52_08255"
     CDS             770..1579
                     /locus_tag="PKZ52_08255"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNG59804.1"
                     /translation="MAVYAVGDVQGCYDSLRRLLDSACFDPACDQLWVAGDLVNRGPD
                     SLGALRFIKGLGQSARMVLGNHDLHLLAVRFSQRAVNKKDTLQPILNAPDGDELLHWL
                     RRQPLMHEGLGYVLCHAGIPPVWSQPQALSLAQEVNAALSGDNFAELLANMYGNQPDT
                     WRDDLRGFERLRLIVNYFTRMRFCTRDGRLDLKAKEGVESAPDGFAPWFNWPGAHLAK
                     PVLFGHWAALEGVTGKPAAIALDTGCVWGKKLSMVRLEDKTWFRVRSELAA"
     gene            complement(1684..1974)
                     /locus_tag="PKZ52_08260"
     CDS             complement(1684..1974)
                     /locus_tag="PKZ52_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59805.1"
                     /translation="MVIKICRLAALAVSLTSCVVVPVAAEKKEGTCGISEDRQTLKVM
                     DFAKDTQSFYSLQGMMVTPISFPTSAVISGVYVSVYNTYKLGETLLVCKKPQ"
     gene            complement(2365..2913)
                     /locus_tag="PKZ52_08265"
     CDS             complement(2365..2913)
                     /locus_tag="PKZ52_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59806.1"
                     /translation="MSSPYEEFLMLLARTKEVFCSSLLACGLIATAACSSIVHNGPRQ
                     FAINSTPEGAKVSAYRQGSSIATDSGVTPKTFSLSARKGYFSGQPYIIKVEKEGFKPV
                     EVKLKPEISGWYWGNLFFGGLIGMLIVDPATGAMWNLAVDTGDKKLDRAQRKKAAKDP
                     VLVVKLVGETTQAERDKMVPIQ"
     gene            3403..4287
                     /locus_tag="PKZ52_08270"
     CDS             3403..4287
                     /locus_tag="PKZ52_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNG59807.1"
                     /translation="MVQVQALLKLCVRIGFACALLLGVTSPAQAAGARHVFIISFDGG
                     KPSVMQASAMPLFKQMAQQGASTMQAFTVVPSITLVSHTSMLTGVQPNKHLIDWNEWS
                     PEKGLVQVPTIFALAKKHGLITGLIAGKDKFKHLNTANSLDLFAIPAYAAQEVATTAA
                     EFIRTKKPNLTFIHFADSDGAGHEFGWGSEQQKAAFAAEDSALEQVRKAITQAGIERD
                     SVIILTADHGGHDKTHGSTRLDDMHIPWVAWGKGVRPGFVLKQRVNTYDTAATALWLL
                     GVAIPKEFDGVAVKEAFN"
     gene            4603..5718
                     /locus_tag="PKZ52_08275"
     CDS             4603..5718
                     /locus_tag="PKZ52_08275"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNG59808.1"
                     /translation="MSEHQPQSATTAIKGRGATSQIVGRFETQSSQREDDGWCQDEPT
                     SPLLTQVSAEVAKSILTRNQSPDLPFSVSLNPYRGCEHGCIYCYARPSHAYLGLSPGL
                     DFESRIFAKINAAQLLRQELAQPNYVPAPIAIGVNTDAYQPCEKTWRITEQVLQVLKE
                     CRHPCGLITKSALVERDIALLTDMAQDNLVTVAITLTTLDADISRILEPRAASPQRRL
                     KTIGRLAAAGIPVSVSVAPMIPFITEQDLEAVLTASAAAGATGANYTLIRLPWEVKPL
                     FTEWLQAHFPDRAERVMNRIRDSRGGKENDSDFTQRMKGEGIFAQLIQRRFNLCAKRL
                     KLNQGREFTALNGQLFRKPSVVPAKRQLVPGQADLFG"
     gene            complement(5813..6100)
                     /locus_tag="PKZ52_08280"
     CDS             complement(5813..6100)
                     /locus_tag="PKZ52_08280"
                     /inference="COORDINATES: protein motif:HMM:NF039469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6482 family protein"
                     /protein_id="HNG59809.1"
                     /translation="MQQATRALIFSYSDSLFYFVGTTADSAGHSDNVQFYKDEAGNLL
                     KFRSLFHAKQFLFTQGFTKGALVMQSAYDEMIGNEPAQKAELPWLYEEAVA"
     gene            6392..6643
                     /locus_tag="PKZ52_08285"
     CDS             6392..6643
                     /locus_tag="PKZ52_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HNG59810.1"
                     /translation="MQLSAANYSRIIEMAWEDRTPFEAIDALYGMREADVIVFMRQAL
                     KPKAFRNWRARVRHRATKHLALRSPAVNRGYCPTQYKQR"
     gene            6679..>6861
                     /locus_tag="PKZ52_08290"
     CDS             6679..>6861
                     /locus_tag="PKZ52_08290"
                     /inference="COORDINATES: protein motif:HMM:NF021526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HNG59811.1"
                     /translation="MAHLKPHLPTKICPVCKRQFVWRKKWRRAWPGVIYCSERCRGAK
                     EPLKNYLTRPSVVEFGK"
BASE COUNT         1745 a         1728 c         1685 g         1703 t
ORIGIN      
        1 tgcatcatga cccagtattt ttgccaggtg gaacatttat ttgatgtggg gcctgaatgt
       61 tttcgcccgg tgccaaaggt ggaatccgcc attgtgcgcc taacccccca ccggcaatta
      121 ccctttcaag ccaagagcca cgaggccctt gagggggtgg tgcgcacggc cttctcccag
      181 cggcgcaaaa ctctgcgcaa cgcgctgaaa ttagtgttta acgatcagca gcttgaagac
      241 gccggtttaa accccaactt gcggcctgaa aatgtggcct tggcgcagta tgtgcagttg
      301 gccaatacct atactcagat ccatggcaat agtgtactaa gcgcagctgg gctccctgag
      361 taaccagcag cgttaaagaa tttacgaggt tatttatgca aactgatgat attcaggtgt
      421 ccatcaaaac ccagtacctc tccgaccaat cggagccggc gcaagagcgc tttgtgtttg
      481 cctacaccat taccatcgcc aacaaatccg accaaccggc ccagttaatc agccgccact
      541 ggattattac cgatggcaat aaccaggtgc aggaggtgca gggcataggt gtggtgggcc
      601 aacagccccg cctggaccca ggcgccagct atacctacac cagcggcgtg gtattaccta
      661 ccggcatggg caccatgacg ggcacctatc aaatgcgttt ggataacggc gatgaattcg
      721 acgcgccgat tccaaccttt gccctaatcc acccgcagag cctgcactag tggcggtgta
      781 cgcggttggc gatgtgcagg gctgttatga cagcctgcgc cgcctgctcg atagtgcctg
      841 ttttgacccc gcctgcgacc aactctgggt ggcgggggat ttggtcaatc gtggccctga
      901 tagtctcggc gctctgcgct ttattaaagg cctaggccag agcgctcgca tggtgttggg
      961 taatcacgac ttgcatctac tcgcagtgcg ctttagccag cgggcagtca ataaaaaaga
     1021 caccctacaa cccatattaa acgcccccga tggcgatgaa ttgctgcact ggttgcgcag
     1081 gcagccactc atgcacgagg gcttaggtta tgtgctctgc cacgccggta ttccaccggt
     1141 gtggagccag ccccaggccc taagcctcgc tcaggaggta aatgcggcgc tcagcggcga
     1201 taattttgct gagttattag caaacatgta cggcaaccag ccggatacct ggcgagatga
     1261 tcttcgcggc tttgagcgcc tgcggctaat cgttaattat ttcacccgca tgcgtttttg
     1321 tacccgcgat ggccgtttag acctcaaggc aaaagagggc gttgaaagcg cccctgatgg
     1381 atttgcccca tggtttaatt ggcccggtgc ccatttagca aaacccgttt tatttggcca
     1441 ctgggccgcg ttagagggcg ttacaggcaa gcccgcagcg attgcgctgg acaccggctg
     1501 tgtatgggga aaaaaactga gtatggtgcg tttagaggat aaaacctggt tccgcgtgcg
     1561 tagcgaattg gctgcgtagc gaatgggctc agagtcttgg cttattaacc cagcgggtta
     1621 atagtagcgt gttgaaatgg gccgccagag tcagcgttaa catttcaaca ggctgttagc
     1681 tttttattgc ggctttttac acaccaacag ggtttcgccg agcttatagg tgttgtacac
     1741 gcttacataa acgccggaaa ttacagcgct ggtgggaaat gaaatgggtg tgaccatcat
     1801 gccctgcaag gaataaaaac tttgtgtgtc tttggcaaag tccattactt ttagagtttg
     1861 cctatcttct gagataccac aggtgccttc ttttttctct gcggccaccg gcaccacaac
     1921 acaagacgtt aagcttactg ctaaggcggc gaggcgacaa attttgatta ccatatcaat
     1981 ctcctagtat ttaaagtgat ctacattttt ggattctttc cagcggatta aaactcttag
     2041 tgggctaata tctttttgca gttagtgaag aatatagcag tattaataag aggaacgccg
     2101 agggaaagag caggctaaaa agagtaaaga ttattgcacc tattgccgag ataaggaact
     2161 gccaagtaag tctggaggca gcaattttga aactcttgcg cctgagatac agaggcttac
     2221 aggaagcaac atatgcgttt acagattgtt cctaagtgcg ggtttaatca gagcatcctt
     2281 aatttaataa tcaacccttc gccaataatt attttagtaa agcgcttaag ccttgtaaga
     2341 cgctgaccta agcgctttaa ttttctactg aataggcacc attttatccc gctcagcttg
     2401 agtggtttct ccaactaact taacaaccaa tactgggtct ttagcggctt ttttgcgctg
     2461 tgccctatcc agctttttat cgccagtatc cacagctaaa ttccacatgg ctcctgtggc
     2521 tgggtcaaca attaacatac cgattaaacc gccaaagaat aaattgcccc aataccagcc
     2581 cgaaatctct ggtttgagtt ttacttcaac gggtttaaag ccctcttttt ctactttaat
     2641 gatataaggc tggccagaaa aataaccctt gcgggcactt aaggaaaatg ttttgggcgt
     2701 aacaccacta tcagtggcaa tactagaacc ctgcctatag gccgagacct tagcgccttc
     2761 tggcgtgcta ttaatagcaa attgccgggg gccattgtgg acgatactac tgcaagcggc
     2821 cgtggcaatt aggccgcagg ctaataaaga tgaacaaaaa acttcctttg tgcgtgctaa
     2881 caacattaaa aattcctcat aagggctact catatgatag ccagggccaa taccctggta
     2941 ttgccaattt agcaggcact taataaaaag caactttgcc gaatcaggtt gtgacttaat
     3001 ctatccctcg cctaacacaa aaatgtatat accaatttag gccttcaaaa gaaggatcgt
     3061 cttgccaagc ctacaaggat attcactggg tgtctgaacc catgtgtatt cattacacat
     3121 ggcagagata gccagctaat ttgaatttgc gggcctatta ttaacctggc ggcaaaatag
     3181 atcgtcctga tctattttgc cgtcgcggcg ttaaacttac gaaaatgcgt ggattttctt
     3241 gttttaacgc agctcgcggc ggtttacacc gccgagcgcg gcatccttgc cgcgatcatt
     3301 aacccggcac taaatgccgc cgggttaata attgccgaca ggttacatag gttcaataaa
     3361 acactactat gttgcaccgt tcattaacct ttaggagttt tcatggttca ggttcaagcc
     3421 ttgctgaaac tatgtgtgcg cataggtttt gcctgcgcac tgttgttagg tgtaaccagt
     3481 cctgcccagg cagctggcgc tcgccacgtg tttattatca gttttgacgg cggcaaaccc
     3541 tcggtgatgc aggccagtgc tatgccattg tttaaacaga tggcacaaca aggcgctagc
     3601 accatgcagg cgtttacggt ggtgcccagt attaccttgg tatcacacac ttctatgctc
     3661 accggtgtgc agccgaataa acatttaatt gattggaatg aatggagccc ggaaaaaggc
     3721 ttggtgcagg tgcccaccat ctttgcattg gccaaaaaac acggcttaat taccggttta
     3781 atcgctggca aagataaatt taagcattta aatacggcca attcattgga cctatttgca
     3841 attcccgcct atgcagccca agaggttgcc acaacggccg ctgaatttat tcgcactaaa
     3901 aaacccaacc taacgtttat tcattttgcc gattccgacg gcgcaggaca tgaatttggc
     3961 tggggctctg agcagcaaaa agctgcgttt gccgcagagg atagcgccct tgaacaggtg
     4021 cgcaaggcaa taacccaggc gggtattgag cgcgacagcg tcataatcct cactgccgat
     4081 cacggcggcc acgataaaac ccacggcagc acccgcctgg acgatatgca tattccctgg
     4141 gttgcctggg gcaagggggt gaggccgggt tttgttctca agcagcgggt taatacctac
     4201 gatacggccg ccaccgcatt gtggttattg ggtgtggcca tacccaaaga atttgatggt
     4261 gtagcggtga aggaggcatt taattaatct attaacccgg cggcaaaata gatcgtcctg
     4321 atctattttg ccttcgctgc actaaactta cgaaaatgcg cggattttct tgttttagtg
     4381 cagctcgcgg cggctcgcac ctttgatgcg gcatctatgc cgccattatt aacccggcac
     4441 taaatgccgc cgggttaata aaagccacat tagggtttaa ctgcggtttt taagataaag
     4501 cccgcttaag ggttaggttt gctttttgct gcaataacgg catttgccaa acgccgctag
     4561 gcggtgcaca atactgtata ttaaaccagt attggggcgg ccatgtccga acatcagcca
     4621 caaagcgcca ccacagcaat caagggccgg ggcgccacca gccagattgt gggccgcttt
     4681 gaaacccaga gcagccagcg cgaggacgat ggttggtgtc aagatgagcc gaccagccca
     4741 ctgcttaccc aggtgagcgc cgaagttgct aaatctattc tcacccgcaa ccaatcccca
     4801 gatttgccct ttagtgtttc actaaacccc tatcgaggct gtgagcacgg ctgtatttat
     4861 tgttacgccc gccccagcca cgcctattta ggcctatcgc cagggctgga ttttgaaagc
     4921 cgaatattcg ccaaaataaa tgctgcgcaa ttattgcggc aggaactcgc ccagccgaat
     4981 tatgtacccg cacctatcgc catcggcgtg aataccgatg cataccagcc ctgcgaaaaa
     5041 acctggcgca ttaccgagca ggttttacag gttttaaaag aatgccggca cccctgcggg
     5101 ttaattacta aatccgcgtt agtcgagcgg gatatagccc tcttaacaga tatggcacag
     5161 gataatttag tgacggtggc cataaccctc accaccctag atgccgacat cagccgtatt
     5221 ttagagcccc gcgccgcctc cccccagcgg cgcttaaaaa ccattggtcg cttggccgcc
     5281 gccggtattc cggtgagcgt cagtgtggcg ccgatgattc cttttattac cgaacaggat
     5341 ttagaagcgg tattaacggc aagtgcagcg gctggcgcca cgggcgctaa ttacacccta
     5401 attaggctgc cctgggaggt taagccttta tttacagagt ggttgcaggc acattttccc
     5461 gaccgcgccg agcgggtgat gaatcgcatt cgcgacagcc gcggcggcaa agaaaatgac
     5521 agtgatttta cccagcgcat gaagggtgag ggcatattcg cgcaattaat tcagcgcagg
     5581 tttaacctgt gtgctaagcg tttaaagcta aaccaagggc gggagttcac tgcattaaat
     5641 ggtcagttat ttcgcaaacc ctcggtggta ccggcaaaac gccagttggt gccagggcag
     5701 gcagatttat tcggctgaca ggatattaac ccggcgataa atatatcccc agaagttcct
     5761 ttgtttaacc gcctgttgca atacgtcgcc gcctaggtat tttaacaagc tgtcaggcta
     5821 cagcctcttc ataaagccaa ggcagctcgg ctttttgcgc cggttcattg ccaatcattt
     5881 catcataggc agactgcatc accaaagcgc ccttggtaaa accctgagta aataagaatt
     5941 gtttggcgtg aaatagactg cgaaatttca acagattacc agcctcatcc ttataaaact
     6001 gcacattgtc tgagtggcct gcactatctg ccgtggtgcc aacaaaataa aataatgaat
     6061 cgctgtagct aaaaattaaa gcgcgcgtag cctgttgcat aggtagctcc aaatcggtaa
     6121 gtggataaaa attctagctg caagagaaag ttctgttcca tcaactacac cgccaaggct
     6181 cagcaacaga gtagcctaat gtgttttatt aagttttaag tggcagtatg tatacgggtt
     6241 ttaagtctta cgcctgccca atgaatttag actgccgcag tgcacgcaag agagtggtta
     6301 tttgatccat agcctacgct cattcgtatg aatgcccatg gataaaatga gccgaccaat
     6361 acgagcccct atgaaaacag caactcaccg cctgcaatta tcagcagcga attactcgcg
     6421 cattattgaa atggcctggg aggatcgcac accttttgaa gcgattgatg cactgtatgg
     6481 tatgcgcgag gcggacgtga ttgtttttat gcgccaagcc cttaaaccca aggcctttcg
     6541 caattggcgg gcgcgggtgc ggcaccgcgc caccaaacat ctagccctgc gctccccggc
     6601 cgttaaccgt ggttattgtc caactcagta taaacagagg taaataatgc atcgtttgcc
     6661 tgcttggcgg cgcagttaat ggcgcacctt aaacctcatt tgccaacaaa aatttgccca
     6721 gtgtgcaaac gccaatttgt ttggcggaaa aaatggcggc gggcttggcc tggggtaatt
     6781 tattgcagcg agcgctgcag gggagctaag gaacctctga aaaattacct tactcgccct
     6841 agcgtcgttg aatttggcaa a
//
LOCUS       DAOQGS010000178         6849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5416_length_6849_cov_4.224168, whole genome shotgun sequence.
ACCESSION   DAOQGS010000178 DAOQGS010000000
VERSION     DAOQGS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5416_length_6849_cov_4.224168"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKZ52_08295"
     CDS             complement(<1..483)
                     /locus_tag="PKZ52_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG59812.1"
                     /translation="MSVLPQIQTLKTWLESQILGQPQLIERILIALIADGHLLVEGAP
                     GLAKTKAIKTLADGIEADFHRIQFTPDLLPSDVTGTDIYRPETGTFEFQSGPIFHNLV
                     LADEINRAPAKVQSALLEAMAERQISVGKSTYRLPPLFLVMATQNPIEQEGTYPLPEA
                     Q"
     gene            691..5448
                     /locus_tag="PKZ52_08300"
     CDS             691..5448
                     /locus_tag="PKZ52_08300"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNG59813.1"
                     /translation="MSANTRFDFPRSFSAALAWASAQVPEASRPVFSQFCRVFSENIP
                     PEDWRDRAPADGGALLLALWQSLYGQVAQERTVLLLNERVSGLGAVPQGSMILVLQND
                     RPFLVDSLRLAFTRLRLPIHLLKNAVFTAERDQNGNLISLQGSGAKQSFIYIESKHLT
                     PQEQQALWADLSEVFEDIAAMVADYGAITEGVSRLEQQLAHAPVPAELISDAQAFLHW
                     LKAGHFTFLGLRQFTLLEGNGGRQLVEDDSARLGVFRHIAPSTGGQWGEGIELFYRQD
                     ELIAFSKSSTRCRVHRLVYPDYVVVKLFNPQGQIVGEIRILGLFTYPVYTLNPWEIPL
                     VNRKVNQILARANFDASSYLGKQLQRVIENYPRDELFQANLDELTETILGVARINERR
                     VVRLFFRFDEFGRFATVVAYIPRDVYNTQLRIQMETLLGEALKSQALDSTTAFSESIL
                     ARAFMVFRLGSDSPKAVDCAALEEAVRQLARPWEEELIEVIQGQLADANALVEHYSQA
                     FSAAYQASFTPAQALTHIQILQAQALEPLRLAIAQHNAQWVFTLFNNGASLALSDVVP
                     ILEHLGLRVLGEHSYLIAPIEQGVKQEIYLHQFTVQPLFAQGRSEEEFSALFIPAFKA
                     IWQKRCDSDAFNRLILAAAIPYFAANMLRAYAAYLKQTLFFLSPEAISDALLKHLPAT
                     AALWQLFDYKFNPHRVSQEQALAEKNSVLRYLETVSQLNDDKILRRLLELMDATLRTN
                     LYQFTGAEPKAYIALKLATARLADVPEPKPAFEIFMYGPQVEGVHLRTSQVARGGLRW
                     SDRLQDYRTEVLGLVKAQQVKNAVIVPSGAKGGFVPKLIPSDASRDQIQALGIAAYRT
                     FISALLDITDNYQGQAITRPPQVICYDEPDPYLVVAADKGTATFSDIANGISADYGHW
                     LKDAFASGGSQGYDHKGMGITAKGAWVAVERHFRELGRSTFNEDFTVVGIGDMSGDVF
                     GNGMLLSEHICLLGAFNHLHIFIDPSPDPRVSFKERQRLFNLPRSSWSDYDPALISPG
                     GGVFLRSAKAIAISEPMRARLGIHAEQLTPNELICALLQAPVDLIWNGGIGTYVKSSQ
                     QTHADIGDKANDGLRVDGGQLRCKIFGEGGNLGMSQLGRIEYCLNGGACNTDFIDNAG
                     GVDCSDHEVNIKICLDELMASGQLGLAERNDLMRAMTDEVAQLVLNNIQRQTQALSFA
                     ANEVKSRMAEYVRFIEELERQGRLQRRLEFLPSAKALSERSHLTRPELAVLLSYAKVM
                     LKDVLLAPGIYNDPYLAQVALGAFPASLQSRFADALGRHRLRPQLVATQLANEMVNNL
                     GITTPQRLKETSGANWEELAKAYTAAREVLQFGRFQALLMDSPQFPAAQQMAWFAAMS
                     RRLRRLTRWYLRAGTQALETQVASHRAGLQAVNHALRNFSAEQLPALNGYQSRVLALI
                     DAGVPEDLAEILAMPEDLFSHLGIVQQIDRAQTGIAQPAEVYTTLAVNYYRLKEHLQV
                     AGFIRALAAIPISNSWQAQVRDAYLLEVEHCLSQLALKGLNRALDQWFAPAAELLARW
                     QAVTDDIEKQGLEDFAVFAVAMGLLAEIAKRVA"
     gene            5495..5746
                     /locus_tag="PKZ52_08305"
     CDS             5495..5746
                     /locus_tag="PKZ52_08305"
                     /inference="COORDINATES: protein motif:HMM:NF022644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 domain-containing protein"
                     /protein_id="HNG59814.1"
                     /translation="MQLHFVQFDLAISAAEYQALYGGWVRQVLVRARDGRKVQLPCNI
                     LQPFVEHRGVYGTFEVGFTAEGKFHSIRRLTQQDVPSGI"
     gene            complement(5855..6073)
                     /gene="infA"
                     /locus_tag="PKZ52_08310"
     CDS             complement(5855..6073)
                     /gene="infA"
                     /locus_tag="PKZ52_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNG59815.1"
                     /translation="MSKEDNIEFDGEVVDTLPNTTFRVKLENGHIVTAHISGKMRKNY
                     IRILTGDKVKVEMTPYDLSKGRITYRAR"
     gene            6465..>6849
                     /locus_tag="PKZ52_08315"
     CDS             6465..>6849
                     /locus_tag="PKZ52_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNG59816.1"
                     /translation="MRQSLLISGLVVLLSLTACSGEPPGASGSSSSGSSSSSSGITSS
                     SSGSSSSTSSSSSSSSSSSSSSSSSSSSSSSGTAWVLEESQAGFCSFNGALGTTHSGF
                     TGSGYVDGSNQTGVALAWAVNAPAAG"
BASE COUNT         1440 a         1864 c         1976 g         1569 t
ORIGIN      
        1 ctgggcttcc ggcagcgggt aggtgccttc ctgctcaatg gggttttggg tggccatcac
       61 caagaatagg ggcggtaaac ggtaggtgga tttacccacg ctgatctgcc gctccgccat
      121 ggcttccagc agggcggact gcaccttggc gggagcgcgg ttaatttcgt cggcgagcac
      181 taggttgtga aaaattggcc cggattggaa ttcaaaagtg ccggtttcgg gccggtaaat
      241 gtcggtgccg gtcacatccg agggcaataa atccggggta aattggatgc ggtggaagtc
      301 ggcctcgatg ccgtcggcca gggttttgat cgctttggtt ttggccaatc ccggcgcccc
      361 ctcaaccagt agatggccat cggcaatcag tgcgatcaga atgcgttcga tcaactgggg
      421 ctggccgaga atttggcttt ccaaccaggt tttaagcgtt tgaatctgag ggagaacaga
      481 catgaacgaa acaggcctcg ataagggtaa aagggatgtt gaatgtgggc gcactgaccc
      541 atagggtgcg tcgtaagttc cttcgaagat agctatgctt aacctagccg ttccatctac
      601 gtgcaattcc atccacgcgc aatacacccc gcgccatgtg gggccatagt ggcaaatttc
      661 aataggatcc attccatagg aagcttaaga atgtcggcca acacccgttt tgattttccc
      721 cgctcgtttt ctgccgcact cgcctgggcg agcgcccaag tgccggaagc ttcgcggccc
      781 gtatttagcc agttctgccg tgtatttagc gaaaatattc cccccgagga ttggcgcgat
      841 cgcgccccgg ccgatggcgg cgcgctgctg ctggcgcttt ggcaaagcct gtacgggcag
      901 gtggcccagg agcgcacagt gctgttgctc aacgagcggg taagcggcct gggagccgtg
      961 ccccagggca gcatgatact ggtgctgcaa aacgaccggc ccttcttagt ggattccctg
     1021 cgtctggcct ttacccgcct gcgcctgccc attcatctgc tcaaaaacgc cgtgtttacg
     1081 gcggagcggg atcaaaacgg taatttaatc agcctgcaag ggagcggggc gaagcagtcg
     1141 tttatttata ttgagagcaa acaccttacg ccccaggagc agcaggcact ctgggcggat
     1201 ttaagcgagg tgtttgagga tatagccgcg atggtggctg actacggcgc cattaccgaa
     1261 ggcgttagtc ggttggagca acaattggcc catgcgccag tgcccgccga gttaatcagc
     1321 gacgcgcaag cctttttaca ctggttaaaa gccggccatt tcaccttttt aggcctgcgc
     1381 caatttacct tgcttgaggg gaatggcggt cggcaattgg tggaagacga ttctgcccgg
     1441 ctgggggtgt ttcgccatat cgcgccctcc acaggcggcc agtgggggga gggcatagag
     1501 cttttttacc gccaggacga attaatcgcc ttttctaaat cctccacccg ctgccgagtg
     1561 caccgcttgg tgtacccgga ttatgtggtg gtgaaattat ttaaccccca ggggcaaatc
     1621 gttggtgaaa tacggatttt gggtttattt acctaccctg tgtacaccct aaatccctgg
     1681 gaaattcccc tggttaatcg caaggtcaat caaattttgg cgcgggcaaa ttttgatgcg
     1741 tcgagttatt taggcaaaca actgcagcgg gtgattgaaa actacccgcg ggatgaatta
     1801 ttccaagcca atttagatga gcttaccgaa actattttgg gtgtggcgcg cattaatgag
     1861 cgcagggtgg tgcggctgtt tttccgcttc gatgaattcg gccggtttgc cactgtagtg
     1921 gcctatattc cccgcgacgt gtacaacacc caactgcgca tccaaatgga gacgctgctg
     1981 ggggaggcct taaaatccca ggcgttagat agcaccaccg cattttccga atccatttta
     2041 gcccgtgcct ttatggtatt tcgcttagga tcggacagcc ccaaagcggt ggactgcgcc
     2101 gccctggaag aggccgtgcg gcaattggcg aggccctggg aagaggaatt aattgaggtc
     2161 atccaaggcc agctggccga tgccaacgct ctagtggaac actatagcca ggcgttttcc
     2221 gccgcctacc aggcgagttt tacccccgcc caagccctta cccacattca aattttacag
     2281 gcccaagcct tagaacccct gcggctggcc attgcacaac ataacgccca gtgggtattt
     2341 accctattta ataacggcgc gagccttgcg ctttccgatg tggtgccaat tctggagcac
     2401 ctaggtttgc gggtattggg tgaacactct tatttaattg cgccaattga gcaaggtgtt
     2461 aaacaggaga tttatttgca ccaatttacg gtgcaaccgc tctttgccca ggggcgcagt
     2521 gaagaagaat ttagcgcttt atttatcccc gcgtttaaag ctatttggca aaaacgctgc
     2581 gatagcgatg ccttcaaccg gttaattttg gcagcggcca ttccttattt cgccgcgaat
     2641 atgctgcggg cctacgcagc ttatttaaaa cagacgctgt tttttttaag ccccgaggca
     2701 attagtgacg ccttattaaa acacctgcct gccaccgccg cgctctggca attatttgat
     2761 tataaattta acccgcacag ggtctcccag gagcaggcgc ttgccgaaaa aaacagtgtt
     2821 ttacgctatt tagaaactgt ctcccaatta aacgacgata aaattttgcg ccgcctgttg
     2881 gaattaatgg atgccaccct gcgcacgaat ttatatcaat ttactggcgc tgagcctaaa
     2941 gcctatatcg ctttaaaatt ggctaccgcg cgcctagccg atgtgcccga gcccaaaccc
     3001 gcctttgaaa tatttatgta tggcccccag gtggagggcg tgcacctgcg cacaagccaa
     3061 gtggccagag gcggcctgcg ctggtccgat cgtttgcagg attaccgcac cgaggtgctg
     3121 ggcctggtaa aagcccaaca ggtaaaaaat gccgtgattg tgcccagcgg cgccaagggc
     3181 ggttttgtac ccaaactcat ccccagcgat gccagccgtg accagattca agccttaggc
     3241 atagcggctt accgcacttt tatcagcgct ctgctggata ttaccgataa ctaccaaggc
     3301 caagcgatca cgcggccgcc tcaggttatc tgttacgacg aaccggaccc ttatttagtg
     3361 gtggcggcgg ataagggcac cgccaccttt agtgatattg ccaatggtat ctccgccgat
     3421 tatggccact ggttaaaaga tgccttcgcc agcggcggct cccagggtta tgaccacaaa
     3481 ggcatgggca tcactgccaa aggcgcctgg gtggcggtgg agcggcactt tcgcgagctg
     3541 ggccgcagca cctttaatga agatttcacc gtggtgggta taggcgatat gtccggcgat
     3601 gtgttcggca acggcatgtt gctctccgag catatctgcc tgttgggcgc ctttaaccac
     3661 ttgcatattt ttatcgaccc aagcccagac ccgcgcgtca gttttaaaga gcgccagcgc
     3721 ctgtttaacc tgccgcgctc cagctggtcc gattacgacc cggccctgat cagccccggt
     3781 ggcggcgttt ttttgcgcag cgccaaggcc atcgccatca gcgaacccat gcgggcaagg
     3841 ctcggcatcc atgccgagca attaacgcca aatgaattaa tttgtgccct gctgcaggcg
     3901 ccggtggatt taatctggaa tggcggcata ggtacctatg ttaaaagcag ccagcaaacc
     3961 cacgccgata taggcgataa agccaacgac ggcctgcggg tggacggcgg gcagctgcgc
     4021 tgcaaaattt ttggcgaggg cggcaacctg ggcatgagtc aactgggccg catcgaatat
     4081 tgccttaacg gcggcgcctg taatacggac ttcatcgaca acgcaggcgg tgtggattgc
     4141 tccgaccacg aggtgaatat caaaatctgc ctggatgaac tgatggccag cgggcaatta
     4201 gggcttgctg agcgcaatga tttgatgcgc gccatgactg atgaggtggc ccagctggtg
     4261 ttaaacaata tccagcggca aactcaagcg ctcagcttcg cggccaacga agtgaaaagc
     4321 cgcatggccg agtatgtccg ttttatagag gagctggagc gccagggccg cctgcagcgg
     4381 cgtttggagt ttttacccag cgccaaagcc ttaagcgagc gcagccatct cacccgcccg
     4441 gagcttgcgg tgctgctctc ctacgccaag gtgatgctca aagacgtatt gttggcgccg
     4501 ggcatttaca acgacccata cctagcccag gttgctctgg gggcattccc cgcctctttg
     4561 caatcgcgct ttgccgatgc cctgggccgc caccggctgc ggccgcaatt ggtggccacc
     4621 caattggcta acgaaatggt caataacctg ggcatcacca cgccccagcg gctaaaagag
     4681 accagcggcg ccaattggga ggagctggcc aaggcctaca ccgctgctcg tgaggtgttg
     4741 cagtttggcc gctttcaggc cctgctgatg gactcgccgc aattcccggc cgcccaacaa
     4801 atggcctggt ttgccgccat gagccgccgc ctgcgcaggc tcacccgctg gtatctgcgc
     4861 gccggtaccc aggcgctgga aactcaggtt gccagccacc gggcgggttt acaggcggtg
     4921 aaccacgccc tgcgcaactt cagcgccgag caactgcccg cccttaacgg ctatcaaagc
     4981 cgggtgctgg cattaatcga tgcaggtgtg ccggaggact tggcggaaat actggccatg
     5041 ccggaggatt tattcagcca tctgggcata gtgcagcaga tcgacagggc gcagaccggc
     5101 attgcccaac cggctgaggt ttataccacc ttggcggtga attattaccg gctcaaagag
     5161 cacctgcagg tggccggatt tatccgcgcc ttggcggcaa ttcccatcag caacagttgg
     5221 caagctcagg tgcgcgatgc ttatctgctg gaggtggaac actgcctcag tcaattggcg
     5281 ctcaagggct tgaaccgggc gttagaccag tggtttgcgc ctgcggcgga gctgttagcg
     5341 cgctggcagg cggtgactga cgatatagag aagcagggct tggaggattt tgccgtgttt
     5401 gccgtggcta tgggcctatt ggcggaaatc gccaaacggg tggcctagag ccgcacgggt
     5461 gatataggac atgggtgata ggggagcaga cacgatgcag ctgcactttg tgcaatttga
     5521 cctggctatt tcggcggcgg agtatcaggc gctctatggc ggctgggtgc gccaggtgct
     5581 ggtgcgcgcc cgcgacgggc gcaaggtgca gttgccctgc aatattttgc agccctttgt
     5641 tgagcaccga ggcgtctacg gcaccttcga ggtggggttt acagcggagg gcaaatttca
     5701 ctcaatccgt cgcctaacgc agcaggatgt gccgtcgggg atttaggcgc gcggtggcgg
     5761 gggtgggcca taggctgggt gcggaagcta atccgccggg gagcgagtgc cttgtgtggt
     5821 caagtcatca aggagcactt tggcagcaga gcgattaacg ggcgcggtag gtgatgcggc
     5881 ctttgctcaa atcatagggg gtcatttcaa cttttacttt gtcgccggtg agaatgcgga
     5941 tgtagttttt gcgcattttg cccgagatgt gggcggtcac tatgtggccg ttttccagtt
     6001 ttacccggaa ggtggtgttc ggaagggtgt ctactacttc gccgtcaaat tcaatattgt
     6061 cttctttcga cattcggctg gttcctcaaa ctcaaatggg ttatttcgcc tgtgctcagg
     6121 ccaaaattag ggcgcgcaat tttgcactaa aagcccctgg cttaaaagct ttaattggca
     6181 gttttttgtg cattcgccgg ggtttgccgt cactgggcgc aaagcctagg cattaaggta
     6241 gcttaggcct ggggcatcgc cccggcggtg aacgctcaac cattgggtta ctcaacaggc
     6301 ttaacggagg ggaatcggca ctggttaacc cgccgagcga caatgtaacc acacttcatg
     6361 acacggattt ttccgccttt ttatggccgt gatttaataa tcccctgcag ctgtgccacg
     6421 gccaacgctc gcaatcctta aatctccgct aggaaccatc ccacatgcgc cagagcctgc
     6481 tcatcagtgg cctcgttgtt ttattaagtc tcaccgcctg cagtggggag ccgccgggcg
     6541 cctcaggttc cagctcgtct ggctcgtcca gttcttccag cggaataacc agctcctcca
     6601 gcggttcatc ctccagtact tcctcgtcca gcagttcaag ctcatccagt agctcaagtt
     6661 catccagttc ctcaagctct tccagcagcg gcacggcctg ggttctagag gagagccagg
     6721 cgggtttttg tagctttaac ggcgccctcg gcaccaccca tagtggtttt accggcagcg
     6781 gttatgtgga tggcagcaat caaaccggtg ttgcgctggc gtgggcggtg aatgccccgg
     6841 cggcgggcc
//
LOCUS       DAOQGS010000179         6840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5425_length_6840_cov_6.430803, whole genome shotgun sequence.
ACCESSION   DAOQGS010000179 DAOQGS010000000
VERSION     DAOQGS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_6840_cov_6.430803"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PKZ52_08320"
     CDS             <1..625
                     /locus_tag="PKZ52_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59817.1"
                     /translation="LATGLVVAASLAANPARSAEADWLDNTYVRLNLLSIKGGESESD
                     FSRNLAATSPSFSLQRYDIDRTGWDLSLGKTLSEHWALELGYKDLGEVQVNFSSRLET
                     PAATAAALADNYPHASGGWTLIAAYSHSLGEQLSLVGRAGLYRWQSRVTINTPSIAPP
                     EDSGTDLLAGLELNYAITPQVGLGVSLERVFVEDGLTSLGFGVKWSF"
     gene            1037..1777
                     /locus_tag="PKZ52_08325"
     CDS             1037..1777
                     /locus_tag="PKZ52_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59818.1"
                     /translation="MKLKGIYLSLVCLALMTSCSSNKPAEKQAAVKPSVLETLTGTWV
                     AEKYLTAIKTNKTPFVDEPESITFSAADKKLTWTNFHEGFSQTIFEAGQESNLYFLNV
                     TAPETAEPPTKKVHFSLLDKTIIFLEPGITKRTNERFVKINEPLPQHANKLLLAGKYK
                     DSEGNIYEFSEDGQAKWPTMSFTYSFVLDRSEANCPYINTNIQNGSGEVVRFGYKWVA
                     GQLYFFDITAEDAPISCSKLPIFSLSKL"
     gene            complement(2090..2623)
                     /locus_tag="PKZ52_08330"
     CDS             complement(2090..2623)
                     /locus_tag="PKZ52_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59819.1"
                     /translation="MKSLIFIIAIFLGGCASVPMASLLELRSFSQDDFMRLQANDIRV
                     KILVDEPVHAVIERTQLTLEATTQKGKNTYAYPLLLLNEVAIKPAAGFFNHTSGKTQY
                     TLKLSDEGIKNVLATQQMIRSDRTAQLKFSINTDFEKLPAEVNEIVLTVSLRLFEQRG
                     FITLFDNAKLSIKPEAN"
     gene            2875..3192
                     /locus_tag="PKZ52_08335"
     CDS             2875..3192
                     /locus_tag="PKZ52_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG59820.1"
                     /translation="MRNKILSIALASLFSLKAYCADIWIDVRSGEEFQAGHLPAAINI
                     PHTEITQRISEVSKDKNDTIHLYCRSGRRSGLALEALQKAGYRQVINEGGYEDLVKKA
                     KAP"
     gene            3527..4300
                     /locus_tag="PKZ52_08340"
     CDS             3527..4300
                     /locus_tag="PKZ52_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="HNG59821.1"
                     /translation="MLTATDLLDLPTCTPAETAIYIVSAKFRDFCDGEQILDYQSALD
                     QLPTIARKVDKIILGQGLGQEHRQRLLESLKFLNAKRSIILHDHIHQRVAKEKVHKVR
                     EHNVMVSTPARIADGLFEAYLVVDEGCAEMSDHQTGEHVQGAVLIEAARQMFMACATS
                     YELSPEASAKIDDMQFALNEMRVVFHNFVFPIETRIELHFEGAEVREKTAEGKARITF
                     YQNDKACCEVFCVAQAFSKKMLKVLETKSARSARRSAIH"
     gene            4311..5405
                     /locus_tag="PKZ52_08345"
     CDS             4311..5405
                     /locus_tag="PKZ52_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59822.1"
                     /translation="MDVLLLEKDGRCIWLENKVLKGLMPMAIRNSVNPFAQKVFLIVD
                     WISNDFGRVGVDLKWLKTLGVPVVEKAKDLPAGNDFKVINTGYDSIVEEEIILRSQGV
                     DIIDKPCPFVRKLRDIFTDHDGSQQIVLLCEDNHIVVKNYASIFPEDLILVQMHNYQE
                     RITIQSNGKPLKLVPYVTFLETDAEEIMAFIRAFAPGQPAVCERTACIWVKSKASPIQ
                     EINQLPADQLQGVKHALLITTANSTNKSVISLENTLKQKGLEVVQIGSLGAFKAFQKN
                     HPQDRVLVVRSPIPNQAEYPIMQYIKHGAVAAFWAALHQSPWLKIAKVKLYKLFYYFY
                     FRLKPQAAKDEAGQFGLLKTAKTAPAGERL"
     gene            5402..6373
                     /locus_tag="PKZ52_08350"
     CDS             5402..6373
                     /locus_tag="PKZ52_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNG59823.1"
                     /translation="MIIGITGATGNLGRRLVQYLLKRLGSNDSIRCLVRSIPTQPLHD
                     ARLSFVQGDLNNSHSLEQFATGLDVCVHLASLVSFATAREYHGVNVAGTALLCEMLMR
                     QAPNCRLVHCSSIAVLRRNSRFTWLNTDYANSKFAGDQVVKNYRQQQGLHSNIIYPGL
                     IYGPEDNNFIPTLGRYLKKGVVFFIRGGERHCPAIFIDDLCELFWQVICRLPASGRDF
                     IGVGKQEVGVHEFIGELAALMHLAKPSTVVPKWLLMPLAIAMDKIYQLAGKKHAPMIS
                     MRSVDLLSINLSPQLVQEFNKDFWTPQVPAQLGLQQSLNWCVQKNLL"
BASE COUNT         1814 a         1744 c         1570 g         1712 t
ORIGIN      
        1 gctggcaacg ggcctcgtgg tagcggcaag ccttgcggcc aacccagccc gcagcgccga
       61 agccgactgg ctggacaaca cctatgtgcg cctgaacctg ctaagcatca aagggggcga
      121 aagcgagagc gacttcagcc gcaacctcgc cgccaccagc ccaagcttta gcctgcagcg
      181 ctacgacata gaccgcacag gctgggacct aagcctcggc aaaaccctca gtgagcactg
      241 ggcgctggag cttggctata aagacctagg cgaagtgcag gtgaattttt ccagccgcct
      301 agaaaccccc gccgccactg ccgccgccct agccgataac tacccccacg ccagcggcgg
      361 ctggacctta atagccgcct acagccacag ccttggcgag cagctaagcc tagtgggccg
      421 cgccggcctc taccgctggc aaagccgcgt caccatcaac accccttcca tagccccccc
      481 ggaggacagc ggcaccgatc tcttagcagg cctagaactc aactacgcca tcacccccca
      541 agtgggcctg ggggtgagtc tggagcgggt gtttgtggaa gatggcctta ccagcctggg
      601 ttttggggtg aagtggagtt tctaaaacag gccacaatcc cactaaagag aaaaagtaaa
      661 aagctcataa acaacttttt ccctatcgcc aaggctatcc tgctccctcc cctttttaag
      721 gggagggttg gggtggggtt tattgacatg cacaaaaccc ggtaaaacct cttaactgaa
      781 tgcgactgcc tgttccccac ctataaatcc caacaaacac aagcctacaa aaatcccccc
      841 gtcaacatcc ccaaagcggc ccaaatcctg tatcgcatct aagatgcttt ttgttaggct
      901 aacggccaac caaatcacct caacaggcaa aaacacttaa ccctcaggtt attgctttta
      961 gcaggcattt attcacagcc atttttaacc cccatcaaca attaattcac acagatcaat
     1021 ttgtttagga gtaacaatga agcttaaagg tatctattta tccctagtct gccttgccct
     1081 gatgactagc tgcagctcca ataaacccgc cgaaaaacaa gctgccgtaa aacccagcgt
     1141 attagaaacc ctcaccggca cctgggtggc ggaaaaatac ctcactgcca taaaaactaa
     1201 taaaacacct tttgtagatg aaccagaatc catcacattc tccgccgctg ataaaaaact
     1261 cacctggacc aattttcacg aaggctttag ccaaaccatc tttgaagccg ggcaggaaag
     1321 caacctgtat tttttaaacg taaccgcccc tgaaactgca gaacccccca ctaaaaaagt
     1381 acatttctcc ctgctcgata aaaccataat ttttttagag ccaggcataa ccaagcgcac
     1441 caacgagcgt tttgtgaaaa ttaacgagcc cttacctcaa cacgccaata aacttctcct
     1501 ggccggcaaa tacaaagata gcgagggcaa tatttacgaa tttagcgaag acggccaagc
     1561 caagtggccc accatgtcct tcacctacag tttcgtatta gaccgctccg aagccaactg
     1621 cccttacatc aataccaaca ttcaaaatgg cagcggcgaa gttgtgcgct ttggctataa
     1681 atgggtggcg ggccaattgt acttttttga catcaccgcc gaagatgccc ccatcagctg
     1741 ctcaaaattg cctattttta gcttgagtaa actttaattt tctatttaaa gtgcttttta
     1801 gtgatgggca tcttggccgc agaaccttaa aaaattgagc agcaataaat cttaaaaaag
     1861 cctgttctgg agtatgtcgg aacagccgca ggcgccttcc gacatttttg agctgacgct
     1921 tgcgaaaatg cgcaaatttt cttgctttag tgcagctcgc agcggtttgc acctttgacg
     1981 cggcatcctt gccgccttta ttaacccggc actaagtgcc gccgggttaa taatcgtcta
     2041 cggatcacgc gcttcaacaa gcgcccaccc ttaaccaaat gtcaattgct tagttggctt
     2101 caggtttaat cgataatttt gcattgtcaa acagagtaat aaagcctctt tgttcaaaaa
     2161 gccttaacga cacagtaaga actatttcat tcacctcggc agggagtttt tcaaaatctg
     2221 tattaattga aaacttcaat tgggcagttc tatcgcttct gatcatctgt tgggtggcga
     2281 gaacattttt tatgccctca tcagatagtt taagggtgta ttgggtttta cccgaggtat
     2341 gattgaagaa acctgccgcc ggtttaattg caacttcatt aagtaacagc aatgggtagg
     2401 catatgtatt cttacccttt tgggtggtcg cttccaaggt taattgtgtt cgctcaataa
     2461 cggcatgaac aggctcatca accaggattt ttactctaat gtcattggcc tgtaggcgca
     2521 tgaagtcatc ttggctgaag gaacgcaatt cgagcagact tgccatgggt acgcttgcac
     2581 aaccgcctaa aaaaatagca ataataaaaa taagactctt catatcctct caatccagtg
     2641 atgatgggtg gcaccctaaa agatatgcca gtaacgtcga tatccaagag cttggcaagc
     2701 cactatggcg acaaggttgc actcccgcta agaacggtta tattttctct tgttcagtct
     2761 aaataaagta ttgactttgc aagcgtgtat acacttaaac ctgctcactg aatttacaat
     2821 gggtctatac agtattcact ccattttatt cgcacctact tttggaaatg cattatgcgc
     2881 aacaaaatcc tatctatcgc cctcgcaagc ctgtttagcc ttaaagccta ctgcgccgat
     2941 atctggatcg atgtgcgcag cggcgaggaa tttcaagccg gccatttacc ggctgccatt
     3001 aatatccccc atacggaaat tacccagcgt atcagcgagg tgagcaaaga taaaaacgat
     3061 accatccacc tgtattgccg ctccggtcgc cgctcggggc tggcgttaga ggctttgcaa
     3121 aaggcgggtt accggcaggt gattaacgag ggcggttatg aggatttagt gaaaaaagcc
     3181 aaagcacctt gataccagtg gccctttacc tgcacgctga agctagggaa cctctgaaaa
     3241 attaccttac tcgctctggc gtcgttgaat ttgccaaaaa aatgctcatt tactgcgtgt
     3301 aaactcggca attgctcctg cattgctcta cctcctgcct ccctgcagtc gtccgctttt
     3361 tttggcaaat tcgcctagcc agagcttcgc cgccgacatt tttcagaggt tccctagtgc
     3421 gcaggcttgg tgaattttta acaaaacccg gtggggttat ctcacatact gcgctgcggc
     3481 gtaaggccta acctatgcct acaccgcaac ccaaggagat aacaccatgt taacagccac
     3541 cgatctttta gacctcccca cttgtacccc agccgaaacc gctatttaca tcgtcagcgc
     3601 caaattccgc gatttttgcg atggcgaaca gatattggat tatcaatcgg cgctggatca
     3661 gttgccgacg attgcccgca aggtggataa aataatcctc ggccagggtt taggccagga
     3721 gcaccgccag cgtttacttg aaagcttaaa atttctcaat gctaaacgct caattattct
     3781 ccacgaccat attcaccagc gcgttgccaa ggaaaaggtg cacaaggtgc gggagcacaa
     3841 tgttatggta tctacacctg cgcgcattgc tgacggcctt tttgaggctt atttagtcgt
     3901 tgatgaaggc tgtgcggaga tgtctgacca tcaaaccggc gagcatgtgc aaggtgcggt
     3961 attaattgaa gcggcccggc aaatgtttat ggcctgcgcc acctcctatg aattatcccc
     4021 ggaggccagc gctaaaattg acgatatgca atttgcctta aatgaaatgc gggtggtctt
     4081 tcacaatttt gttttcccca ttgaaacccg tattgagttg cattttgaag gcgccgaggt
     4141 gcgggaaaaa accgccgaag gcaaagcgcg cattaccttt tatcaaaatg ataaagcctg
     4201 ctgcgaagtg ttttgtgtgg cccaagcctt ctcaaaaaaa atgctcaagg tattagaaac
     4261 caaaagtgcc cgtagcgccc gccgcagcgc cattcattaa ggagcccgcc atggacgtat
     4321 tactgctgga aaaagacggc cgctgtatct ggctggaaaa taaagtgctt aaaggcttga
     4381 tgcccatggc catccgcaac tcggtcaatc catttgcaca aaaggtattt ctaattgtgg
     4441 attggatctc caatgatttt ggccgtgttg gggtggattt aaaatggctg aaaaccctcg
     4501 gggtgccagt agttgaaaag gcaaaggatc tgcccgccgg caatgatttt aaagtcatta
     4561 ataccggtta tgactccatt gtggaggaag aaattatcct gcgctcccaa ggggtggata
     4621 ttattgataa accctgcccc tttgtgcgca aactgcggga tatttttacc gaccacgatg
     4681 gcagccaaca aattgtgctg ttgtgcgagg ataaccatat agtggtaaaa aattacgcta
     4741 gcattttccc ggaggattta atcctggtac agatgcataa ttatcaagaa cgcatcacca
     4801 tccaaagcaa cggtaagccg cttaaactgg tgccttatgt taccttttta gaaaccgacg
     4861 ctgaggaaat catggcgttt attcgcgcct ttgcaccggg gcaaccggct gtttgtgaac
     4921 gcaccgcctg tatttgggta aaaagtaaag ccagccccat tcaggaaatt aatcaattac
     4981 cggcggatca gttacagggt gttaaacacg cgctgttaat caccactgct aattcaacta
     5041 ataaatcggt aatttctctg gagaataccc tcaagcaaaa aggcttagag gtggtgcaaa
     5101 ttggctccct aggagcgttt aaagcgtttc aaaaaaatca cccgcaagat cgggtactgg
     5161 tagtgcgctc gcccataccc aaccaggcgg aataccccat tatgcagtat ataaaacacg
     5221 gcgctgtagc cgcgttttgg gcggcgctac accagagccc ctggcttaaa attgccaagg
     5281 ttaaactcta taaattattt tattattttt actttcgctt aaagccccaa gcggctaaag
     5341 atgaagccgg gcaatttggt ttactgaaaa cagccaaaac agcaccagca ggagaacggc
     5401 tatgattatt ggtattaccg gtgccaccgg caatttaggt cgccgcctgg tgcagtatct
     5461 gcttaagcga ttgggtagca atgattctat tcgctgtttg gtgcgcagca ttcccaccca
     5521 accgcttcat gacgcgcgcc tgagctttgt gcaaggcgat ttaaacaata gccacagctt
     5581 ggagcagttc gccacaggtt tggatgtgtg cgtgcattta gcatccctag tgagttttgc
     5641 cactgctcgg gaataccatg gggtaaatgt ggcgggcacc gctttattgt gtgaaatgtt
     5701 aatgcgccag gcgcccaatt gccgcttggt gcactgttca tccatcgccg tcttgcgccg
     5761 caactcgcgt tttacttggc taaatacgga ttacgccaac agtaaattcg cgggtgacca
     5821 ggtagttaaa aattatcgcc agcaacaggg cttacacagc aatattatct acccagggct
     5881 tatttacggg cccgaggata ataattttat ccccaccctc gggcgttatt taaaaaaagg
     5941 cgtggtattt tttattcgcg gcggcgagcg ccactgcccg gcgattttta ttgatgacct
     6001 gtgcgagcta ttttggcaag tgatttgccg tttacctgct tccgggcggg attttatcgg
     6061 cgtgggtaaa caggaggtgg gcgtgcatga atttatcggt gagcttgctg cgcttatgca
     6121 tctggccaaa ccctccaccg tggtgcctaa gtggttatta atgcccttag ccatagccat
     6181 ggacaaaatc tatcaactag ccggtaaaaa acatgcgccg atgatttcca tgcgcagcgt
     6241 ggatttactc tcgattaact tatcccccca attggtgcag gagtttaata aggatttttg
     6301 gactccacag gttcccgcac agctcggttt gcagcaatcg ttaaattggt gtgtgcagaa
     6361 aaacttgctt tagtggcctt tttaatttta cctggcggtg caatgtccgc gatgctgcaa
     6421 ctattcagca cactttactc ccctcttccg cgcgggcggc aaattcagga ggatttagcc
     6481 atccggaaaa atcgattcgc ggtgtagcag tggtgactac aggttagtgc agaccgtcaa
     6541 aaagagggac ctttttgacg agcctacacg gatgtattca cggcgtgtct gcactaacct
     6601 gtagtcaccg ctgcggacgc tggattacgg ggccacagca ctaaaacacg catgcgatag
     6661 ttcccggtca gtgcaggaat tacctccagc cacataaaaa gatgctgaat agttacgcga
     6721 tgcttttgtc attgacttat tatggctttt atgccgactc tctggcagtt aaggaagctc
     6781 tgaaaaatgt cggcggcgaa gcgctggcaa ggcgaatttg ccaaaaaaag cggacgactg
//
LOCUS       DAOQGS010000180         6830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5444_length_6830_cov_4.381107, whole genome shotgun sequence.
ACCESSION   DAOQGS010000180 DAOQGS010000000
VERSION     DAOQGS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5444_length_6830_cov_4.381107"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKZ52_08355"
     CDS             <1..1041
                     /locus_tag="PKZ52_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNG59824.1"
                     /translation="STRVVGAQGYSGDVSGGGNAAAITVASLTAMQDAIKAYSGSDGL
                     VLLYNGKFDYAPIRAAICEQWPKDKQEVEIKDKSNITIVGADGSAANFGIRIKGTSKN
                     ILIRNMTIGLLPGGANDGDAIGIEGGSNIWLDHNELFSANQKCPGTPDDDTTFEGLID
                     IKKTADNITVSYNYIHDHWKVGLDGSSDTDTSVRHITYHHNRYENVSARLPLQRFGFT
                     HIYSNYYNGITESGINVRMGGQTLVESNYFYNAKNPVTSRSSDEIGFWDLRNNNIQSP
                     ADFSQYKITWTSGSGAWKNADAWTTTKAFPALPYKYTVDAPAKLDCISKATAGAGKGL
                     KVAADVVSQCGN"
     gene            1961..3487
                     /locus_tag="PKZ52_08360"
     CDS             1961..3487
                     /locus_tag="PKZ52_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59825.1"
                     /translation="MTKTGVQTRLIALSLFSAAALFGCGGGDTGSSSSSSSSSSSSSS
                     SNSSSSSSSSSSSSSSSSSSSSSSSSSSGIVHATCPSTNSAAFQQLPRSVPGQIEAED
                     FDPAGYSDTTSENEDRAYRTDTGVDIKSISGGYAVTKFASGEYIEFSVNVATENDYGL
                     YIRSGALATGAALKVTQCSQTLVDKFDVPVVAQAGQFKTYSVGKIHLKPGLQKIRISS
                     TGSEKPELDWIFLGDYTGTIDKPDTTPGVVKSLGCGKTRSIQDGRRTISVDGTNRTYI
                     LRTPSNYNNQTAYRLIFAYHWLNGNANQVALGGNGGSTDDPFYGLWDQANNTTIFVAP
                     EGIDAGWANTNNRDLKFTDAMLQQIQNDMCIDNSRIFATGFSYGASMSNAIACARASV
                     FRGVALYAGAQLSGCEGGTAPIAYFHAHGIRDSVLNISQGRSLRDRAVKNNKCTAMTP
                     QEPTSGSGTHICTSYQNCTAGYPVRWCAFDGDHNPTEKDRGQTKSWVPGEAWKFISQF
                     "
     gene            complement(4011..5387)
                     /gene="dbpA"
                     /locus_tag="PKZ52_08365"
     CDS             complement(4011..5387)
                     /gene="dbpA"
                     /locus_tag="PKZ52_08365"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HNG59826.1"
                     /translation="MPTAFNSLPLKPELLSTLAALEYTQMTPVQEQSLPLVLKGGDLI
                     GKAKTGSGKTAAFALGILQQLSVEHFHTQALVICPTRELADQVAKEIRTLGRAIANIK
                     VLTLCGGSPIGPQKISLERGAHIVVGTPGRLLDHISKNTLNLGQIKQLVLDEADRMLD
                     MGFSEQLNAIIAACPQHKQTLLFSATYPDDIAAISRRCQKNPSIVSVDDNAPNHAISQ
                     YFYELKSPQEKPRALLKLLAEFTPSSSLIFCNTKQTCNDLLEFLRQNKIAAATLHGDL
                     EQKQRDQILVCFANGSLPLLIATDVAARGIDIKQLDLVINYELAREASVHIHRIGRTG
                     RAGESGTAINLVAPQDLPALHRIEDLTGSPAKLLSLPNPKTPLNLEAAMVTLAIDGGK
                     KSKLRAGDVLGALTKDGAIIGEHIGKISVFDFSIYVAVHKSYWQTALIQLQNYPIKGK
                     TFKARRLM"
     gene            complement(5464..>6830)
                     /gene="lnt"
                     /locus_tag="PKZ52_08370"
     CDS             complement(5464..>6830)
                     /gene="lnt"
                     /locus_tag="PKZ52_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNG59827.1"
                     /translation="SGDFWRALSFNLGLCGAGVSWVYVSISQYGSTTPAVAALLMALF
                     IGGIAFIFSWPYWLGARFFRNNIYALALGFPAVFVLGEWMRSWLFTGFPWLYLGYGLM
                     DTPLLGWAPVFGVFGISLLSLLAAGFLGLAVIKLLRDKNRPQAFLWLLAAIIPGLIGR
                     TILPLHWTHSSEQSISIGMVQPNIPQEEKWDPLYYAQNIKILRELTEPLWGKDWIIWP
                     EAAIPFLYHDAQPFLEQIDQTAKRFNSVFISGIIFDDIQQDKYYNSILARGDGGSGLY
                     FKQRLVPFGEYVPFEEYLRGLIGFFDLPVSIINVGPEQQNGLKARGLLISPAVCYEIA
                     YPDLVARRAKDSNILLTISNDAWFADSIGPLQHFQIARMRAAETRRYVVRATNTGMTG
                     IIDPFGKVQVMGERYKPQAVSGEATGVEGLTPYMVWLNWPVLFACWLLLGWSYFATRP
                     RAIA"
BASE COUNT         1732 a         1607 c         1699 g         1792 t
ORIGIN      
        1 tcaacccgtg tggtgggggc ccaggggtat tcgggtgatg tgtccggcgg tggcaatgcc
       61 gcggccatta cggtggccag ccttaccgcc atgcaagatg ccatcaaagc ctattctggt
      121 agtgacggcc tagtgttgct gtacaacggt aaatttgatt acgcccctat ccgcgccgct
      181 atttgtgaac aatggccgaa ggataaacaa gaggtggaaa ttaaagataa aagcaacatt
      241 actattgtgg gcgccgacgg ctctgcggct aattttggca ttcgtattaa gggcaccagt
      301 aaaaatattc tcatccgcaa tatgaccata ggtttattgc ccggtggcgc caatgacggt
      361 gatgcgatag gcattgaggg cggttcgaat atttggttgg atcacaatga attgtttagc
      421 gccaatcaaa aatgccccgg cacgcccgat gatgacacta cctttgaagg cttaatcgat
      481 attaaaaaaa ccgcggataa cattaccgtt tcctacaact acattcacga ccactggaag
      541 gttggtttag acggttccag tgatacggat acttccgtgc gccacattac ctaccaccac
      601 aaccgttatg aaaatgtttc cgcacgcctg cctctacagc gctttgggtt tacccatatt
      661 tatagcaact actataacgg cataaccgag tcgggcatta atgtgcgcat gggcgggcag
      721 actttggttg aaagtaatta tttttacaat gccaaaaacc ccgttacctc gcgcagtagc
      781 gatgaaattg gcttctggga tttacgcaac aataatattc aaagcccggc tgatttctct
      841 caatataaaa ttacctggac cagcggcagt ggcgcttgga aaaatgccga tgcctggact
      901 accactaaag cattccccgc gctgccctat aaatacacag tggatgcacc ggcgaaatta
      961 gactgtatta gcaaggcgac agcaggtgct ggcaagggtt taaaagttgc cgccgatgtg
     1021 gtaagtcaat gcgggaacta agctagatta aattccgttt tgggtttttg tcttttgaat
     1081 gatgccctgc attttaatac ggcaattccg tttaaggtgc agggtttttt atgcataaat
     1141 ggcctggggt tattattaga atttaattgt aaccattcag taagcctgat tatctctctc
     1201 gtgcgggcag caaatttcgc aggctttagc catagggcat attcgattcg cggtgtggca
     1261 gtggtgactt attgcaggcc gtcaaaaaga gggacctttt tgaccagcct acacggatgt
     1321 atttacggcg tgttcgcact aagctctagt cacaactgca aggacagtat tacggggttg
     1381 cagtactaaa agacgcggat gatagttccc agttagcgcg gcaattaact ccagcgacac
     1441 aaaaagatgt ttaatagtta caattatttt aaccctataa attacccgct tttgcgccgc
     1501 tagtgagttt gattaattgc gcatcactct tcgggcatat tggcaaaatg aatataagct
     1561 cgcatcaggt aatctaattt ttgccttctc gcgtcgcctg ctaggcggcg ttattgtcgt
     1621 ccgcagtcgg tctgtttacc gcataaataa tctttgccgc ataaatgctg tccatcgcgc
     1681 gaatatcacg acaattaaat tttaatggtg taaggcctgc acaaattggc gggctgtgca
     1741 aattccattt cacttggtcc gcaattttca aaacctttga taacaattat cagataatgc
     1801 gtggttggtc gcagaaaatg ttccgtcgtg gtttatgatg cattcatcat acaatatacc
     1861 caatgccatc tcccggcccg caaaataagt actataaaaa ttcgctttct cggtattttg
     1921 tgtgagcaac ttcacgttat cctttcatag agtaaacatc atgacgaaga ccggcgttca
     1981 aaccaggctt atcgcgcttt ctcttttttc tgcagctgcc ctttttgggt gtggcggtgg
     2041 agacacgggc agttcatcct cctccagcag ttcttctagc agctcaagta gctccaacag
     2101 ctcttcatcc agcagtagct caagcagttc atcatcttct tctagtagtt catccagcag
     2161 ctcatcgagc agctcgggca ttgtgcacgc tacttgcccc agcacaaatt ctgcagcctt
     2221 tcagcaactc ccgcgatctg tgccgggcca aattgaagcg gaagattttg accctgccgg
     2281 ttactccgat accacatctg aaaatgaaga ccgcgcctac cgaaccgata cgggcgtaga
     2341 tattaaatct atttctggcg gttatgcggt tactaagttc gccagcggtg aatacattga
     2401 atttagcgtt aacgtcgcca ccgaaaacga ctatggttta tatattcgct cgggcgcatt
     2461 agcgacaggc gccgcattaa aagtgaccca atgcagccaa acactggtcg ataaatttga
     2521 tgtgcccgtg gtggctcaag cagggcaatt taaaacctac tcggtgggta aaattcactt
     2581 aaaacccggc ctgcaaaaaa ttcgcatcag ctctaccggt agcgaaaaac ccgagttgga
     2641 ttggatcttt ttgggtgatt acacaggtac cattgacaag cctgatacca cgcctggggt
     2701 tgtgaaatcc ttaggttgcg gtaaaacccg atccattcaa gatggccgca gaaccatctc
     2761 cgtagacggt actaaccgca cctatattct gcgcacaccg tctaattaca ataaccaaac
     2821 cgcctaccgt ttaatcttcg cttaccactg gcttaatggt aatgccaacc aggttgcgct
     2881 tggcggtaat ggtggttcta cggatgatcc gttctacgga ttgtgggatc aagctaataa
     2941 caccactatt ttcgttgcac cagaaggtat agatgccgga tgggcgaata ccaacaaccg
     3001 cgatttaaaa ttcaccgatg ccatgttgca acaaattcaa aacgatatgt gtatcgataa
     3061 ctcacgcatt tttgctaccg gatttagcta tggcgcttcc atgtccaacg ccattgcctg
     3121 tgcccgtgcg agtgtgttcc gtggggttgc cttgtacgca ggtgctcaat tgagcggttg
     3181 tgagggtggc actgcaccga ttgcttactt ccacgcccat ggtatccgcg actcggtatt
     3241 aaatatttcg caaggccgca gcctgcgtga tcgcgcggtt aaaaataata agtgtacggc
     3301 tatgactccg caggagccca ccagcggcag cggtacccat atttgtactt cctaccaaaa
     3361 ctgcaccgcc ggttacccag tgcgctggtg tgcgtttgac ggcgaccaca acccaacgga
     3421 aaaagatcgc ggtcagacca aatcctgggt gcccggcgaa gcttggaagt ttatttcgca
     3481 gttttaattt aggttgggtt ggtatttagc taaacccact taaaccttaa aagccctgca
     3541 cctttggtgt ggggcttttt tttgggcgaa tttaatattt agtatttcgt aattattccg
     3601 caccttttta ttgtctgcag ctttttagtg gtgctggtcg tgattctcta gctgactaga
     3661 aaacaccaga tcagttatgt ggtgctgcga acccgcagtg atgaatacag gttattgcag
     3721 acacgccgtg aatacatccg tgtcggctcg tcaaaaaggt ccctcttttt gacggtctga
     3781 aataacctgt attcagcact gctgtacttc gaatcgacta ggcaggttgg ttaaaccttc
     3841 cttaatttgc catccgagca gagagcgcag taatgtctgc tgaatagtta gagtattcca
     3901 agctaaaaaa ccgcagtgga gtgcaacagc ggtatttagg tgcaacaagc tgccggtttt
     3961 gcggctatgt agggacggcc aaaaaggagt gctgaataat tacaggtttc ttacatgagc
     4021 ctgcgcgctt taaaggtttt gcctttaatc ggatagtttt gcaattggat tagggcggtt
     4081 tgccagtagc ttttatgcac ggccacatat atcgaaaaat caaagacaga aattttacct
     4141 atatgctcgc caataatggc gccgtctttg gtgagtgcac ctaaaacatc accggcgcgc
     4201 agtttggatt ttttgccgcc gtctatggca agcgtaacca tggcggcctc taaattcaga
     4261 ggtgtttttg ggttgggcag gcttagtagc ttcgcagggc taccggttaa gtcttcaatg
     4321 cgatgcaggg cgggaagatc ctggggggcc actaaattaa tcgctgtgcc actctcgcct
     4381 gcacggcccg tgcggccaat gcggtgaatg tgcacagacg cctccctggc cagctcataa
     4441 ttaattacta aatctaactg tttgatatcg atgccgcgag ccgcaacatc ggtggcaatt
     4501 aataagggca agctgccgtt ggcgaaacaa accagtattt gatcccgctg tttttgttct
     4561 aaatcaccgt gtagtgttgc agcggcgatt ttgttttgcc gtaaaaattc cagcaggtca
     4621 ttgcaggttt gtttggtgtt gcagaaaatt aaactgctgc tgggggtaaa ttcggccagt
     4681 aattttagca gtgcgcgggg tttttcctgg ggggatttta attcgtaaaa atattggctg
     4741 attgcgtggt tgggtgcgtt gtcatctacg ctgacaatac tggggttttt ctggcagcgg
     4801 cggctgatcg cggcaatatc gtctggataa gtagctgaga acaacagggt ttgtttgtgt
     4861 tgcgggcagg cggcgatgat ggcatttaat tgttcgctaa accccatgtc taacatgcgg
     4921 tcggcttcgt cgaggactaa ttgttttatt tggccaaggt tgagtgtgtt tttgctgatg
     4981 tgatccagca ggcgcccagg tgtgcccaca acaatgtgcg cgccgcgctc taaggatatt
     5041 ttttgcggcc ctatagggct gccgccacac agtgtgagca ctttaatatt ggcaatggcg
     5101 cgcccgagag tgcgaatttc tttggccacc tggtcggcca actcccgtgt tgggcagatc
     5161 acaagggctt gcgtatggaa atgctcaacc gaaagttgtt gcaaaatacc cagggcaaag
     5221 gctgcagttt ttccactgcc ggttttagcc ttgccaatta aatcaccgcc ttttaaaacc
     5281 aggggtaggc tctgctcctg caccggtgtc atttgggtat attctagggc ggcaagtgtg
     5341 ctgagtaatt cgggttttaa gggcaagctg ttaaatgcag tgggcatggg aattccaaat
     5401 aagggtaggg gcggcgatgg ctgcagctta attttggcga ggctattttt tttagtgagt
     5461 cgattatgcg atcgctcgag gtcgtgtggc aaagtaactc cagcccaata atagccaaca
     5521 ggcaaataat accggccaat tgagccaaac catatacggc gtaaggcctt caactcctgt
     5581 ggcttcaccg cttacagcct gcggtttata gcgctcgccc attacttgca ccttgccaaa
     5641 tgggtcgata ataccggtca taccagtgtt ggtggcgcgc accacataac ggcgggtttc
     5701 ggcggcgcgc atgcgggcga tttgaaaatg ttgtaagggg ccgattgaat cggcaaacca
     5761 ggcgtcgttg gaaatggtga gtagaatatt gctgtctttg gcgcggcggg cgactaaatc
     5821 cgggtaggca atttcataac acacggcggg ggatattaat aaaccccgcg cttttaaacc
     5881 attttgttgc tctggcccca cgttaataat agagacaggt aaatcaaaaa aaccaattaa
     5941 gccgcgcagg tactcttcaa agggcacata ttcgccaaac ggcactaaac gctgtttaaa
     6001 gtacaggccg ctaccgccgt cgccacgggc taaaatactg ttgtagtatt tgtcttgttg
     6061 gatatcatca aaaataatgc cgctgataaa cacggaatta aagcgtttag cggtctgatc
     6121 gatttgttcc aaaaacggct gagcgtcgtg gtacaaaaag gggatggcgg cctccggcca
     6181 tatgatccag tctttacccc agaggggttc ggttaactcc cgtaaaattt taatattttg
     6241 cgcgtagtag agtgggtccc atttttcctc ttggggaata ttcggctgca ccataccgat
     6301 ggatatagat tgttcgctgc tgtgtgtcca gtgcaggggc aatattgtgc ggccgattag
     6361 gccgggaata atggctgcca gcaaccacaa aaatgcctgc gggcgatttt tatcgcgcag
     6421 gagtttaata acagcgaggc cgagaaatcc ggcggctaat aagctaagca ggctaatacc
     6481 aaacacgcca aataccggcg cccagcctaa aagcggcgta tccatcagcc catagcctaa
     6541 ataaagccag ggaaaaccgg taaatagcca ggagcgcatc cattcaccca atacaaaaac
     6601 cgccggaaag cccagcgcca aggcgtatat attgttgcga aaaaagcgcg cacctaacca
     6661 atagggccag ctaaaaataa acgcaatacc gccgataaat agcgccatca agagggctgc
     6721 cacggccggt gtggtgctgc catattggct gatgctgaca taaacccagg agacgcccgc
     6781 gccgcacagg cctaaattaa aactcagcgc ccgccaaaaa tccccgctgg
//
LOCUS       DAOQGS010000181         6821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5450_length_6821_cov_4.887230, whole genome shotgun sequence.
ACCESSION   DAOQGS010000181 DAOQGS010000000
VERSION     DAOQGS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_6821_cov_4.887230"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..1488)
                     /locus_tag="PKZ52_08375"
     CDS             complement(313..1488)
                     /locus_tag="PKZ52_08375"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNG59828.1"
                     /translation="MTVTRTLGPLHFEDLDPKRFEDLIRQLAYEFKNWRRLEATGRAG
                     SDDGFDARGYEITNEDLSGESEEEMITLAQSDRLWLIQCKREKSIPPAKLTKYLEDFV
                     LQEDEKLYGMVFTAACDFSKRARDLFREKCEAKGIREWHLWGRAELEDFLFQPRNDHL
                     LFAYFGVSLTIRKRAQKSEIRSRLAIKRKLQRVLENSSHSAVMLRSPDAIEYPNSRAI
                     PGFKKDPVWVLRKYKGMMHAGLQFTLRRHFAYISGDEMTWDAALAQNDAFAHDDPWGE
                     HLDDSEVRQKILEFWESQPAVNQAWFELYGVVSFDSIVDVDELGDEIIKAPHIYVPFR
                     GLDGPFSSYSAVVEHKLGGDIKLYPISLEDRRVEIFHEEWRKCGAYEVLTGISPQDD"
     gene            complement(1660..4173)
                     /locus_tag="PKZ52_08380"
     CDS             complement(1660..4173)
                     /locus_tag="PKZ52_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG59829.1"
                     /translation="MSDYKLSTLWIEAFAIKGDGLDEKRAYLVAAYEKLRERVAVLVE
                     DIRPDMRGLTVHDITHIDALWEIASTIAGSSYPINAAEAFVLGGAFLLHDAAHCVAAY
                     PDGITGLNATAEWKQVCTRHKLDPLNMAADSPEYKKVIFDVLRLLHPKRARDLLGAKW
                     RGQYLLEDSDLREDFKEVVGAVAESHWFNTSQLEQFNKETLTCPASLAPANWRIDKLK
                     IAILLRTADAAHIDSRRAPTFLRAIMNPNGVSGLHWEFQNNMWQVGLKEDSDELYVSS
                     RPFSLEQRAAWWLAYDTAQMIDKELIDADRLLKEFRSESDRLKATNFKSARSPDLFCV
                     TVPTKGWQPVDTSIKVTNIKKLVQTFGGEKLYGPEPKWALRELLQNSVDAVHALRAIG
                     GLGKEEGEIEVSLETVGDHYWLHITDNGVGMGKFVLSEVLVDFGRSLWKDGALSREWE
                     TLVGTDFKSIGQFGIGFFSAFMLGDRIRVISRRYERVEGEPQSWVMEFDDGLESRSLL
                     RSARPDESLLRAGTKVSIFLTSQKLKKLCSLKGYDKNLSEILLYLAPALDINLRYKDQ
                     NCSARQLIAANDWKSMPAHDLTERIKFHSIERENLYSELKNDEGDIIGRVGISTHRVL
                     SYGVGVVGGIFAGPLDYLEGLVICGDQNDVARKSAVPIANFSNFIDWAKTQKSLLLAA
                     NKLTLNLSGALNVFGCAIDDMIVVEDLQHGNPIYYADFVQMLANLQGEIKICVDSVDW
                     NEDDEVSSAAYDSFAADENVFIGVKSYEYGWLENINKEAAEYGKELQINFYEKMLDGL
                     PDLKLARRIVMATIGYVGEVSIERACLVVTRAQPAQINE"
     gene            complement(4297..5559)
                     /locus_tag="PKZ52_08385"
     CDS             complement(4297..5559)
                     /locus_tag="PKZ52_08385"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNG59830.1"
                     /translation="MLSWDDYEDTLETAIPAIAAQNAAAQMAAQQTLQAADKPEPQPA
                     APKQDYKPATNSDAVARAQQAVADLDPAPGLEELAMGAARVQVDDKRMINCRADLNQL
                     VPFKYEWAWQKYLDGCANHWMPQEINMTADIALWKSPEGLTDDERTLVMRSLGYFSTA
                     DSLVANNLVLAIYRHITCPEARQYLLRQAFEEAIHTHAYQYCIESLGMDEGEVFNMYR
                     EVPSVAKKAAWSIAHTRGISSPMFHTGTTETDQELLRNLIGFYVVTEGIFFYCGFTQI
                     LSMGRRNKMTGVAEQFQYILRDESMHLNFGIDVINQIKLENPSLWTESFKDEVKQMVL
                     EGTELEIAYARDTMPRGVLGMNAAMMEEYLHFIANRRLAQLGLSEAFPGAQNPFPWMS
                     EIMDLRKEKNFFETRVIEYQTGGALSWE"
     gene            complement(5887..6327)
                     /locus_tag="PKZ52_08390"
     CDS             complement(5887..6327)
                     /locus_tag="PKZ52_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59831.1"
                     /translation="MSFWDKVKSFTRSAKCTVGWHGGEYAPIPGKPKCHLGKTCPNCN
                     DYLTKEEHEFSEWIFTQEGDCNARRECIYCGYEEQEEKHNHEQVAKDLSNCEILLRCR
                     RCGDEKVGPAEHEWVEQPQAQAQGTTDSASGNKKQCQVCGIISP"
     gene            complement(6384..6617)
                     /locus_tag="PKZ52_08395"
     CDS             complement(6384..6617)
                     /locus_tag="PKZ52_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59832.1"
                     /translation="MATLVPDESTDTGAYWTLDYVYKAGCTVNIRCGYADKTSVDINL
                     AEKIQKCSYKIDAMKKSSVLPIEIINFERYIKG"
BASE COUNT         1742 a         1734 c         1362 g         1983 t
ORIGIN      
        1 tatcgtagta gacaaaacca ataatatata aagcgggcgc cacgagggcc aaggcggggg
       61 tgatgttgac gagccaatta aaatttgcgc gttcggatga cattcaatct ccttgtgggt
      121 aagggcgtta ctttttgtca gtttaaccac gccatcccga atatggctaa tcaattttgc
      181 agtgagcggc gccgggcgcc gggcatatac gcatcgcgat tttggtacga tttttatatc
      241 gctcaaagca actattcggc gcgccaaagt gcgccttagg acgtaaattt gtgcgggtta
      301 ctcaattttc tcttaatcat cttgaggaga tataccagtt agcacttcgt atgccccaca
      361 ctttcgccat tcttcgtgaa aaatttctac ccgcctatct tctaagctaa ttggatacaa
      421 tttaatatcc ccgcctagtt tatgttcgac aacagcagaa tagcttgaaa atggtccatc
      481 aagaccgcgg aatggtacat agatatgcgg ggcctttata atttcatctc ccaattcatc
      541 aacatcgacg attgaatcaa acgatactac gccatataac tcgaaccacg cttgatttac
      601 agcaggctgg ctttcccaaa attcaagtat tttttgtcta acttctgagt catctaaatg
      661 ctctccccat ggatcgtcat gcgcaaaagc atcattttga gcaagagcag catcccatgt
      721 catctcgtct ccagatatat atgcaaaatg ccgcctcaac gtaaattgca atcccgcatg
      781 catcattcct ttatactttc ttaacaccca aaccggatct tttttaaatc caggaattgc
      841 tctagagttc ggatattcta tcgcgtctgg cgagcgaagc ataacagcag aatgcgaaga
      901 attttccaag actcgctgga gctttctttt tattgccaat cttgaccgaa tttccgattt
      961 ttgcgcccgc ttgcgaatag ttaacgacac tccaaaatat gcaaagagca gatgatcatt
     1021 ccttggctgg aaaaggaagt cttctagctc ggccctcccc caaagatgcc attctcgaat
     1081 tcccttcgcc tcacatttct cacgaaatag gtcccgggct cgcttagaga aatcacaagc
     1141 agcagtaaaa accataccat aaagtttctc gtcttcctgg agtacaaaat cttcaaggta
     1201 cttggttagt ttggcagggg gaatcgattt ttcgcgtttg cactgtatga gccataagcg
     1261 atcgctctgg gccaaggtga tcatctcttc ttctgactca ccactaagat cttcgttagt
     1321 tatctcgtac cctcgtgcgt caaatccatc atctgagcca gctcttccag tcgcttcgag
     1381 tcggcgccaa tttttaaatt catatgccaa ttgacgtata agatcttcaa atcgcttggg
     1441 atctagatct tcaaagtgaa gcggtccaag tgttcgagtc actgtcattt gcatccccat
     1501 ttcggtagtt tcaatataag cgcgagaata ttgcttactt gctcaaccac caacagccct
     1561 ccttggccat ggtgggcgca acgacttgta tctttcctac ttccttggag cgcataaggg
     1621 tcgaacaaga tattcaattg ggcgtcccag ggcacactat tactcattga tttgtgcggg
     1681 ctgagcacga gtcacaacca aacacgccct ttcaatagaa acctctccga cgtaccctat
     1741 ggtagccata acgattcgcc tggcaagttt taaatctggc aaaccatcaa gcatcttttc
     1801 ataaaaatta atttgtaact ccttcccgta ttccgccgcc tccttattaa tgttctccaa
     1861 ccatccatat tcgtaacttt taacaccaat aaaaacattt tcatctgcag caaagctgtc
     1921 ataagcagca ctagaaacct catcgtcttc attccagtca actgaatcaa cacagatttt
     1981 tatctcacct tgcaaattcg caagcatttg gacaaaatca gcataataga tcggattgcc
     2041 gtgctgcagg tcttctacaa caatcatgtc gtcgattgcg caaccgaata catttaaggc
     2101 gccactgaga ttcaatgtca gtttattagc agccaaaaga agtgatttct gagtcttcgc
     2161 ccaatcaata aaattggaaa aattggctat tggaactgca cttttcctcg caacgtcatt
     2221 ctgatcgccg catattacca gcccctccaa atagtcaagt ggtcctgcaa aaattcctcc
     2281 taccacacca actccataac ttaaaaccct atgcgtagaa attccaactc taccgattat
     2341 gtcaccttca tcatttttga gttcagaata taaattctcc cgttcaatag aatggaattt
     2401 tattctttct gttagatcat gcgcaggcat agatttccag tcgttcgctg ctattaactg
     2461 cctcgcagag caattttgat ccttgtacct taggttgata tctagagcag gcgcaaggta
     2521 aagcaatatt tccgacaaat ttttatcgta acccttcaag gaacatagct tttttaattt
     2581 ttgacttgtc aggaatatag atactttggt accggctcta agtagcgact cgtcaggtct
     2641 agcacttctt agcagtgatc tactctctaa tccatcgtca aattccatta cccaagattg
     2701 cggctctcct tccactcgct cataccgcct acttattacg cggatacgat cacctaacat
     2761 gaatgcggag aaaaaaccta tgccgaattg acctatagac ttaaaatcgg tgccgacaag
     2821 agtttcccac tctctcgata acgcaccgtc cttccataag gatctaccga aatccacaag
     2881 cacctcagat agcacaaatt tacccatgcc gaccccgttg tcggtgatgt gaagccaata
     2941 gtggtcgcct acggtctcga gcgatacttc tatttcaccc tcctccttac ccaaaccacc
     3001 aattgcgcgc agcgcgtgca ccgcatccac tgagttctgt agcagctctc taagcgccca
     3061 cttgggttca gggccatata atttttcgcc accaaaggtt tgcaccaatt ttttaatatt
     3121 tgttactttt atacttgtat cgacaggctg ccagccttta gttggaacag ttacgcagaa
     3181 taaatcaggt gatcttgcag acttaaagtt cgtcgctttt aatcgatctg attcactccg
     3241 aaactctttg agcagacgat ctgcatcaat taattccttg tcgatcatct gtgctgtatc
     3301 ataggctaac caccacgctg cgcgctgctc caacgaaaat ggcctactac taacatataa
     3361 ttcgtcagag tcttccttta aacctacctg ccacatatta ttctgaaact cccaatgaag
     3421 tccggaaaca ccattcgggt tcattatggc gcgaagaaaa gttggtgccc gccttgaatc
     3481 aatatgagct gcatctgcag ttctaagcaa tatcgcaatt ttcaatttat cgattctcca
     3541 atttgctggc gccaaactgg caggacaggt tagagtttct ttattgaact gttccaactg
     3601 ggaggtatta aaccagtgac tttccgcgac cgcaccaaca acctccttaa aatcctcgcg
     3661 caaatcagag tcttccagca aatattggcc ccgccatttt gcacccaata aatctcttgc
     3721 gcgcttagga tgcaacaacc tcaatacatc aaagataact tttttatatt caggtgaatc
     3781 ggcggccata tttaatgggt ctaatttatg gcgtgtgcat acctgcttcc attctgcagt
     3841 agcatttaag ccagtaatgc cgtctggata ggcagccacg caatgtgctg catcatgcaa
     3901 taaaaacgcg cctcccaaaa cgaaagcttc tgcagcattt attggataac tcgaaccggc
     3961 aatggtagaa gcaatttccc acagtgcgtc aatgtgcgta atatcgtgaa cggtgaggcc
     4021 gcgcatatca gggcggatat cttctacgag aaccgcaacc ctttctctaa gtttttcata
     4081 agctgcgacc aaataggctc gcttttcgtc caatccatca cctttaatgg caaatgcctc
     4141 aatccaaagc gtgctcaatt tgtaatctga catcttactc tccataaaaa agccgcaaga
     4201 gttaatttct ttttagaaat ttacacattt gcggccaaca gcattattta gttacactat
     4261 agagtgtgta acacacaact ctctactaaa ctcaatttac tcccaagaaa gcgccccacc
     4321 cgtctgatac tcaatcaccc gcgtctcaaa aaagttcttc tctttacgca aatccataat
     4381 ctcactcatc caaggaaacg gattctgcgc gccggggaag gcttcagaca gccccaactg
     4441 cgccaaacgg cggttggcga taaagtgcag gtattcttcc atcatggcgg cgttcatgcc
     4501 cagcactccg cgcggcatgg tatcgcgggc gtaggcgatt tctaattcgg tgccttctaa
     4561 taccatttgt ttgacttcgt ctttaaacga ttccgtccac aggctggggt tttctaattt
     4621 aatttggttg attacgtcta taccaaaatt taaatgcatg gattcatcgc gcagaatata
     4681 ttggaactgc tcggcaaccc cggtcatttt atttcggcgg cccatgctga ggatttgcgt
     4741 aaaaccgcag tagaagaaaa taccttcggt caccacataa aaaccaatta aattgcgcag
     4801 caattcttga tccgtctcag tggtgccggt gtggaacatg gggctggaaa taccgcgggt
     4861 gtgggcgatg ctccaggcgg cttttttggc taccgatggc acttcgcggt acatattaaa
     4921 tacttcgcct tcatccatac ccaaggattc aatacagtat tgataggcgt gggtgtggat
     4981 ggcctcttca aaggcttggc gcaataaata ttggcgcgct tctgggcagg tgatatggcg
     5041 gtaaatggcc agcactaaat tattggcaac taaagaatcc gccgtggaga aataaccgag
     5101 tgagcgcata actaaagtgc gctcgtcatc ggttaggccc tcgggggatt tccatagggc
     5161 tatgtcggcg gtcatattaa tttcttgcgg catccagtgg ttggcgcagc cgtccaaata
     5221 tttttgccag gcccactcgt atttaaacgg caccaattgg tttaaatcgg cgcggcagtt
     5281 aatcatgcgc ttgtcatcca cctgcacacg ggcagcgccc atggccagct cctccaaacc
     5341 tggggctggg tctaaatccg caactgcctg ttgggcgcgg gcaactgcgt cgctgttggt
     5401 tgcgggtttg tagtcttgct tgggggcggc aggctggggt tctggcttgt cagcggcctg
     5461 cagggtttgt tgcgcggcca tttgggcggc tgcattttga gcggcaatgg caggaatggc
     5521 ggtttctaat gtgtcttcgt aatcgtccca acttaacatg tgagggctcc tgtgattttg
     5581 tgttgatgaa taggggttgt gtttttacgc tttaattagt ttttttgttt tgttctattt
     5641 gggtttttgc tgttttttcg gttttaatct tgcgccctgg caccgctgcc ttgagattga
     5701 gccagttaaa aatcccaatc ttttgggctt ttattaaata catcttttag aaatcacttt
     5761 cttgacaggg ttgtgattaa tctttgaatt tgaatccgct tgtcggatgg cgctgcgctt
     5821 atccaaccta cgagtaacga ttatttcctt tgcccaggtt ccgagtgttg ggcattactg
     5881 cccaatctac gggctaatta tcccgcacac ctggcactgc tttttattgc cgctggcact
     5941 gtcagtggta ccttgcgctt gcgcttgcgg ttgctcaacc cactcatgtt cagcagggcc
     6001 tactttttcg tccccgcaac gtctgcaacg caacaaaatc tcgcagttgc tcaaatcttt
     6061 tgccacctgc tcgtggttgt gtttttcctc ctgctcctcg taaccgcaat aaatgcactc
     6121 acgcctagca ttacagtcgc cctcttgggt aaatatccac tcagagaact catgctcctc
     6181 tttggttaaa taatcgttac aatttgggca ggttttgccc aaatggcact tgggtttacc
     6241 gggtataggc gcatattcac ccccatgcca acccactgta cattttgccg atcgcgtaaa
     6301 tgattttacc ttatcccaaa aactcatttt tattctcctg attttgttat ctgacctaaa
     6361 gggcgtcacc ccaaagtttg tgtttatccc tttatatacc gctcaaaatt tataatttct
     6421 attggcaaaa cactgctttt tttcattgca tcaattttat aactgcactt ttgtatcttt
     6481 tctgccaggt tgatatccac actggtttta tccgcatacc cgcagcggat attcaccgtg
     6541 cagcccgcct tatacacata atccaaggtc caataagcgc ccgtatcggt tgattcatcc
     6601 ggcaccaatg tggccatatc ggcaaccggg ccatcaaata cttgtactgt ttttacaaca
     6661 gtatccgata ctctgttgtt ttggttttgc ttccgtagcg ctagcaataa tggttaaaac
     6721 aagcaaaaca aaaactttaa agagcggcat ttgttagcac caatagcggc gaagtttttg
     6781 ccttcgcgat tacccgcaac cccctcccca accctcccct t
//
LOCUS       DAOQGS010000182         6798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5483_length_6798_cov_5.256711, whole genome shotgun sequence.
ACCESSION   DAOQGS010000182 DAOQGS010000000
VERSION     DAOQGS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5483_length_6798_cov_5.256711"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKZ52_08400"
     CDS             <1..265
                     /locus_tag="PKZ52_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HNG59833.1"
                     /translation="LTAADYVIHLDPWWNPAVEDQASDRAHRLGQQRPVTIYRLVTQN
                     TIEEKIIALHSEKRDLADNLLAGGEAAGKLDTAALLKLLQDTA"
     gene            912..1514
                     /locus_tag="PKZ52_08405"
     CDS             912..1514
                     /locus_tag="PKZ52_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59834.1"
                     /translation="MLDKKAVESICTAIASDYQGWEYSNKTFKNKSLKYAAKIINFGR
                     SYSGLCSNFQPLLGIWHKKLCQEYKKIFGVSEEWISGNMLYNAVPEYRSFNFFTDYTQ
                     PGHDHSYMEQMIRGVFLRCAQEIDKIYNFDSEAELIESFPVELEFQGGLKHCLARAYL
                     CDFEFIKNYRLRQLEGVSPHYRMNEQGVDQIIKYYKIPME"
     gene            1596..1943
                     /locus_tag="PKZ52_08410"
     CDS             1596..1943
                     /locus_tag="PKZ52_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNG59835.1"
                     /translation="MASPLRLEFAGALYHVTSRGDRREDIFEDDQDREVFLTILGDIY
                     RDRNWIFHAYCLMSNVYHLLVETPDANLAKGMRQLNGVYTQRFNRAHHRVGHVFQGRY
                     KAILVAFIKTTTL"
     gene            complement(2082..2381)
                     /locus_tag="PKZ52_08415"
     CDS             complement(2082..2381)
                     /locus_tag="PKZ52_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNG59836.1"
                     /translation="MVKWTGHAKAQLRHIYDYVAQDSATYAKRVTQELVNRSLSLDEL
                     PRAGKVVAELNNELVREVSLYSYRILYEIKHEVIEVLAVIHKRRDVQADEIPRGE"
     gene            complement(2375..2560)
                     /locus_tag="PKZ52_08420"
     CDS             complement(2375..2560)
                     /locus_tag="PKZ52_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59837.1"
                     /translation="MHTHTLKQDALEILQHLPDNADIEEIMYRLYVLENIRRGQLDAE
                     KGKTTDAEQLLKDIQKW"
     gene            2864..4081
                     /locus_tag="PKZ52_08425"
     CDS             2864..4081
                     /locus_tag="PKZ52_08425"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNG59838.1"
                     /translation="MSSIKKVVLAYSGGLDTSVIARWLQDTYSCEVVTFTADIGQGEE
                     VEPARAKAQALGVKDIYIEDLREEFVRDFVFPMFRANTIYEGEYLLGTSIARPLIAKR
                     LVEIANETGADAISHGATGKGNDQVRFELGAYALKPGIKVIAPWREWDLTSRETLMAY
                     CEQHNIPVDFNKKKKSPYSMDANLLHISYEGGQLENPWTEAEEDMWRWSVSPEAAPDK
                     AEYLEISFQNGDPVALNGVAMSPASLLETLNKIGGAHGVGRLDLVENRYVGMKSRGCY
                     ETPGGSILLKAHRGIESITLDREVAHLKDSLMPRYAEMIYNGYWWSPERLMIQRAIDE
                     SQAHVNGDVRVKLYKGSVTVVGRRSSNSLFDEKIATFEDDAGAYNQKDAEGFIKLNAL
                     RMRIAAQKGRKLF"
     gene            4098..4667
                     /locus_tag="PKZ52_08430"
     CDS             4098..4667
                     /locus_tag="PKZ52_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59839.1"
                     /translation="MKLFTKTVLVAALATASLGALAERPNFNYLEGGYLRHRIDNGCV
                     QDGLKIAGSAEISDRVFVAGSYSDVSDDNKAPPKCGSSMLRAGAGLYGDFSDAASFYG
                     TLSGVQFTPDGGDSDTGWAIEGGFRSFITNEVEIGALVGLHDVGPVNEAYISVNGIYW
                     FSDQLGAYIEASASDESTKGLGLGIRLSF"
     gene            4970..6421
                     /gene="ccoN"
                     /locus_tag="PKZ52_08435"
     CDS             4970..6421
                     /gene="ccoN"
                     /locus_tag="PKZ52_08435"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNG59840.1"
                     /translation="MSSDMAHASAHPQYNYDIVRAFTLATILWGIVGMGLGVFIAAEL
                     VWPGLNDILQPFSHFGRLRPLHTNAVIFAFGGCALFATSYFVVQRTCQATLFGGWLIP
                     FTFWGWQAVIVAAAITLPLGYTSAKEYAELEWPIDILITLVWVAYAIVFFGTIVKRQT
                     SHIYVANWFFGAFIITVACLHIVNNMEIPVTLFKSYSIYSGAMDAMVQWWYGHNAVGF
                     FLTAGFLGMMYYFVPKQAGRPVYSYQLSIVHFWALISVYVWAGSHHLHYSALPDWAQS
                     LGMVMSLILLAPSWGGMINGLLTLSGAWHKLRDDPTLRFLVVSLSFYGMSTFEGPMMA
                     IKTVNALSHDTDWTIGHVHSGALGWVAMISIGAVYHLIPVLTGQKTMWSVKLINVHFW
                     LATIGTVLYVVAMWINGIFVQGLMWRAFNDDGSLTYSFVQAVEASRPGYIMRFIGGAF
                     FVSGMFFMAYNAFRTLQAEKKEAIDNNAQPQLA"
     gene            6432..>6798
                     /gene="ccoO"
                     /locus_tag="PKZ52_08440"
     CDS             6432..>6798
                     /gene="ccoO"
                     /locus_tag="PKZ52_08440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNG59841.1"
                     /translation="MKNHDLVEKNIGLMVVLIVVAISFGALVEIVPHFFSPQTNEPIA
                     GLKPLPAKELEGRDVYIREGCHVCHTQMIRPLRAEVERYGHYSVAGESVYDHPFLWGS
                     KRTGPDLARVGKRYSDEWHK"
BASE COUNT         1618 a         1746 c         1705 g         1729 t
ORIGIN      
        1 cctcaccgcc gccgactacg ttattcacct agacccctgg tggaaccccg ccgtcgaaga
       61 ccaagcctcc gaccgcgccc accgcctagg ccaacagcgc cccgtcacta tttaccgcct
      121 ggtcacgcaa aataccatcg aagaaaaaat tatcgcccta cacagtgaaa aacgcgattt
      181 ggcggataat ttattggccg gtggcgaagc ggctggcaaa ctggatacag cggcattatt
      241 aaagttgtta caggatacgg cgtaggcggc gttaattaat tgggacaaca gccgcaggca
      301 atccaaaggt caaggaatca ctcctctcgg ttgtagtggg aggagagata gccccatgat
      361 tatggcgcga cgtgaacagt aagtgagggg attgacggct agccccatgg attcgctagc
      421 gggtttataa gtcttgtggc atggttgttc aggtatcttg cgttttaaat taggcgaatc
      481 aaggtgtggc gaaaccatcg cgtagccact tgccctctaa accattaaat taaaacactc
      541 gctcaacagg ggttgatacc aactcccccc gcgccctccc ggcggcgggg aatggagttt
      601 ttggggttaa gcgccaaggt ctggcggtgg tgagcgaata aaaaaagcgg ggcattaact
      661 gcggctaaag gaaaatacaa taccggatgt gagccctgaa ttaaattgag gatttttcgt
      721 agggcgtcgc cttggcgcgc cacactgatt actccgataa atgaaagcgg cgcacttaag
      781 tgcgccctac ggttagtgcg ggttacgcgt tggcacaggc agaggaggga acgctgacca
      841 cgccaagttt aatgggcagc caccaccatc ttccattgtt gctctatttg acatttagag
      901 aggggtaaag aatgctcgac aaaaaagcag tcgaaagtat ttgtaccgcc atagcctcag
      961 attaccaagg ttgggaatac tcaaacaaaa cctttaaaaa taagtcatta aagtatgcag
     1021 ctaaaattat taattttggc cggagctata gcggtttgtg ttcaaacttt caaccgttac
     1081 ttggtatttg gcacaagaaa ttgtgtcaag aatataaaaa aatattcggt gtcagtgaag
     1141 agtggattag tggcaacatg ctctataatg ctgttccaga gtatcgcagc ttcaattttt
     1201 tcaccgatta tacgcagcca ggccatgacc actcctacat ggagcaaatg attcgcggtg
     1261 tatttctgag gtgtgcacag gaaattgaca aaatatacaa cttcgattcc gaagcagagt
     1321 taattgaaag ctttcccgtt gaactggaat ttcaaggcgg cctgaaacac tgtctcgcaa
     1381 gagcctattt gtgtgacttt gaatttatta agaactacag acttaggcaa ctagagggtg
     1441 tcagcccgca ctatcgaatg aatgagcagg gcgtagatca gattattaag tattacaaga
     1501 tcccaatgga atgatcagcc agaaacattg aggtcaggtc ttgcgttttg ccttttagca
     1561 ttatttatat ggaaattttt taaaggagat tatttatggc tagccccttg cggttggaat
     1621 ttgccggtgc gctttatcat gtgacatcga ggggcgatag gcgggaagat atttttgagg
     1681 acgaccagga ccgcgaggta ttcctgacga ttttgggaga catttatcga gatcgcaatt
     1741 ggatttttca tgcctattgt ttaatgtcca acgtttatca tttattagtt gaaacgccag
     1801 atgcaaatct cgcaaaaggc atgcggcaac ttaatggtgt gtacacgcaa cgatttaacc
     1861 gcgcccatca ccgcgtcggt cacgttttcc agggccggta caaggccatt ttggtggcat
     1921 ttatcaagac aaccaccctc taagcccagc aaatccgccg cccaatctta ataacaccgg
     1981 gcatggcatg tgagaatggt cttgattttc tgccttttaa tactgaaaca agcccactta
     2041 taagaggcga aacaaaagcc cactacaagg gccatttgta tttattctcc gcgcggaatt
     2101 tcatccgctt gcacatcgcg gcgcttatga atgactgcaa gcacctcaat gacttcgtgc
     2161 ttgatctcat acaaaattcg gtacgagtaa agcgagactt ctcgaactaa ctcgttattg
     2221 agttcagcca caactttacc tgctcgagga agctcatcca gagataatga cctgttgact
     2281 aattcttgtg tcacgcgctt ggcataggtg gcagagtctt gtgcaacgta atcgtatatg
     2341 tggcgaagtt gtgcctttgc atgacctgtc cacttcacca cttctgaatg tccttcagaa
     2401 gttgttcggc atcagtagtt ttaccctttt cggcatctag ttggccacgg cgaatatttt
     2461 ctagcacata caatcggtac atgatttctt caatatcggc gttatctggc aggtgttgca
     2521 gaatttccag tgcatcctgt tttagtgtgt gtgtgtgcat ggtaattcct cggactagct
     2581 attaagggca tatgtacgtt tatcttggct cctgccgtta aaattatcag cactggctga
     2641 ccttcggcag agaagttttc taaatcttgt aactaccttg cagtttaggg ctgtggacac
     2701 cggactgtct aatttatggt gccatcaccg aggacatggc gagcaaactc accaccaccc
     2761 atatggccgc acccaaacca ccgcagcgct acttttttcc ccccgatgcc gctacaatgc
     2821 cgccccaaat tttcgcccct cattagcctt tgttggagcc tttatgtcgt ctatcaaaaa
     2881 agtggtgctg gcctattccg gcggcttgga tacctcggtc atcgcccgct ggctgcaaga
     2941 cacctacagc tgcgaagtgg ttaccttcac cgctgacata ggccagggcg aagaagtgga
     3001 accagcccgc gctaaggctc aggccctggg cgttaaagac atctatatag aggacctgcg
     3061 cgaggagttt gtgcgcgact tcgtgttccc catgttccgc gccaacacta tttacgaagg
     3121 ggaatacctg ctgggcacct ccatcgcccg ccccttaatc gccaagcgct tggttgaaat
     3181 cgccaacgag accggcgccg atgccatctc ccacggcgcc acaggtaagg gtaatgatca
     3241 ggtgcgtttt gaactcggcg cctatgccct caagcccggc attaaagtga tagccccctg
     3301 gcgcgaatgg gatctcacca gtcgcgaaac cctgatggcc tattgcgaac aacacaatat
     3361 tccggtggat ttcaacaaga agaaaaaatc cccctactcc atggacgcca acctgctgca
     3421 tatttcctat gagggcggcc agctggaaaa cccctggacg gaagccgaag aagatatgtg
     3481 gcgctggtca gtgtccccag aggccgcgcc cgataaagcc gaatacttgg aaatcagctt
     3541 tcaaaacggc gacccggtgg ccttaaatgg cgtagccatg tccccggcca gcctgttgga
     3601 aaccctcaat aaaatcggcg gcgcccacgg cgttggccgc ttagatttgg tggaaaaccg
     3661 ctatgtgggc atgaagtcgc ggggctgtta cgaaaccccc ggcggcagca ttttgctcaa
     3721 ggcccaccgc ggcattgagt cgatcaccct cgatcgcgaa gtggcccacc taaaagattc
     3781 actcatgcct cgctatgccg agatgattta caacggttac tggtggagcc ccgaacgcct
     3841 gatgatccag cgcgccattg atgaatcaca ggcccatgtc aatggcgatg tgcgagtgaa
     3901 gctctacaaa ggcagcgtca ctgtggtggg gcgtcgctcc agcaacagtt tatttgacga
     3961 aaaaatcgcg acctttgaag acgatgctgg cgcctacaac caaaaagacg ccgaaggctt
     4021 tatcaagctt aacgcgctcc gtatgcgcat cgccgcgcaa aaagggcgca aactgtttta
     4081 atacttgggg ttcgtttatg aaactattta ccaaaactgt attagtggct gcactagcca
     4141 ccgcttcatt aggagcgctt gcagagcggc ctaattttaa ctatctcgaa ggcggctatc
     4201 tgcgccaccg catcgataac ggctgcgtcc aagacggttt aaaaattgcc ggtagcgctg
     4261 aaatttccga tcgcgtgttt gttgcgggca gctacagcga tgtcagcgac gacaacaaag
     4321 ccccccccaa atgcggctcc agcatgctgc gcgcaggtgc cggcctgtac ggcgacttta
     4381 gcgatgccgc aagtttctac ggcacacttt caggcgttca attcacaccc gatggcggcg
     4441 actccgacac aggttgggct attgagggcg gctttcgcag cttcatcacc aatgaagtcg
     4501 aaatcggtgc cttagtaggc ctgcacgatg ttggcccggt taatgaagct tatatatcgg
     4561 ttaacggtat ttactggttt tctgaccaac tcggtgctta cattgaagcg tcagcttctg
     4621 atgaaagcac taaaggactg gggctcggta ttcgtctgag cttttaataa ttcgtgcgct
     4681 gggcagcgag gccaaactcg ccgcccacgc ttaattgttg cagttgacac caatcaagcc
     4741 tagcgtcgca agtttgcgta taattgctgt tcctgcggca attggtcgta gagcgtttaa
     4801 gcccccggcg cagtttcacg ctccatagtt atgttggcga gatgttgaaa aggcctgaaa
     4861 cgatttaccc gttctgaagt tgcgctttag agcatctagg ttactcgtta gccggaggac
     4921 ttcctccttg cgctaaccca ccattttctt tttacagtgg atcaacatta tgagcagtga
     4981 catggcacac gcctcagcgc accctcagta caactatgac atagtgcggg ccttcaccct
     5041 ggccacgatt ctatggggca tagtcggtat gggcctaggg gtttttatcg ccgcagaact
     5101 tgtttggcct ggccttaacg atattctgca gccgttctcc cacttcggac gcctgcgccc
     5161 cctgcacacg aacgcggtga tctttgcgtt tggcggctgc gcactgtttg ccacttctta
     5221 ttttgttgtg cagcgcacct gtcaggcgac gctgttcggc ggttggttaa tcccgttcac
     5281 cttctggggt tggcaggcgg tgattgtcgc tgccgccatc actctaccct tgggctacac
     5341 ctcagctaag gaatacgccg agctggagtg gccgattgat atattaatta ccctggtatg
     5401 ggtggcctac gcgattgtgt tctttggcac tatcgtcaag cgccaaacat cccatattta
     5461 tgttgccaac tggttctttg gcgctttcat tattaccgtg gcctgcttgc atattgttaa
     5521 caatatggaa atcccggtaa cgctgtttaa gtcctactct atttattccg gtgccatgga
     5581 tgccatggtg cagtggtggt acggccacaa cgcggtgggt tttttcttaa ccgcaggctt
     5641 cttgggcatg atgtattact ttgtgcccaa acaagcgggc cgccccgtct actcctacca
     5701 attatccatt gtgcatttct gggcgctgat atcagtctac gtatgggcgg gctctcacca
     5761 cttgcactac tcagctttac ctgactgggc gcaaagcctc ggcatggtga tgtcactgat
     5821 tcttttggcg ccctcctggg gcggtatgat caacggcctt ttgaccctct caggcgcctg
     5881 gcacaagttg cgcgatgacc ccaccctacg cttcttagtg gtatcgctct ccttctacgg
     5941 catgtcgact tttgaaggcc ccatgatggc aatcaaaact gtaaatgccc tctcccacga
     6001 tactgactgg actatcggcc atgtgcactc aggtgcttta ggttgggtag cgatgatttc
     6061 catcggtgcg gtgtaccact taattcctgt attaaccggc caaaaaacca tgtggagcgt
     6121 aaaactcatc aacgtgcact tctggcttgc caccatcggc actgtgcttt acgtagttgc
     6181 catgtggatt aacggtattt ttgtgcaggg ccttatgtgg cgcgccttta acgacgacgg
     6241 ctccttaacc tacagctttg ttcaagcggt agaggccagt cgcccaggct acatcatgcg
     6301 ctttataggc ggtgcgttct ttgtttcagg catgttcttt atggcctaca acgcgtttcg
     6361 cacactgcag gccgaaaaga aagaggccat cgacaacaat gctcaacctc aattggctta
     6421 aggggtaatt cgtgaaaaac catgatctag ttgagaaaaa tatcgggttg atggtggtgc
     6481 tgattgtagt ggccatctcc tttggcgccc tcgtggaaat cgtgccgcat tttttttcgc
     6541 cgcaaaccaa tgaacccatc gcaggcttaa aaccccttcc cgccaaagag ctggaagggc
     6601 gcgatgtgta cattcgcgaa ggctgccatg tctgccacac ccagatgatt cgccccttgc
     6661 gcgctgaagt tgagcgctat ggccactatt cagtggcggg tgaatccgtt tatgaccacc
     6721 ccttcctgtg gggttctaaa cgcaccgggc cagacctggc tcgcgtaggt aagcgttaca
     6781 gcgacgaatg gcacaagg
//
LOCUS       DAOQGS010000183         6790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5494_length_6790_cov_3.841277, whole genome shotgun sequence.
ACCESSION   DAOQGS010000183 DAOQGS010000000
VERSION     DAOQGS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5494_length_6790_cov_3.841277"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKZ52_08445"
     CDS             <1..931
                     /locus_tag="PKZ52_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNG59842.1"
                     /translation="RDLSIAGRVAAKTASGAIIHPLVDFKRPIAVIPSLAIHLDRSAN
                     EQRSVNPQEDMRPILLQDGSLDFRALLLTQLQAQGHKDVSEIFEYDLCLYDSQPAALV
                     GVQEEFIASARLDNLLSCYLGLQAMLSSAGEQSALLLLTDHEEVGSQSDVGARSNLLN
                     GFLQRLFSSDEERLRALANSMMLSVDNAHGIHPNYPRKHDDNHGPLLNKGPVLKVDAN
                     QGYATSSESAALVRLLAQQAQVPIQSFVTRADMRCGSTIGPMTAALTGVRTADLGVPT
                     FAMHSIRELAGARDPDLMLALLSAFVAAEQLSG"
     gene            1043..2083
                     /locus_tag="PKZ52_08450"
     CDS             1043..2083
                     /locus_tag="PKZ52_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNG59843.1"
                     /translation="MITDDVLARFEHFDKLTPHLLLDALDALEFLRIDEGGFLFKRGK
                     SLPEAYYLLDGSLDLIDSNFNTSSISAGSPEALCALNSDPVTAVTARATSRVLAFAIN
                     REHLSRLVTYSQSSPQLSSERAPGADAIELMEDDDWFERLLDSHLFSRIPMAHVQQLF
                     TRLRSIPVKAGEKIMREGERGDYFYVLASGNAVITNALGTIRVDLTPGMSFGEEAILG
                     KTPRNATVTMLTDGVIKRLTEEDFNELIRRPVMQYIEASAVEALSESFTVLDVKMPIE
                     FRCGHYPGAINMPLARLRDQLKDLATSKLYLVPDDAEGRAEIAAHILCQAGFNVRIIK
                     GAAEVIYGERAG"
     gene            2135..3142
                     /locus_tag="PKZ52_08455"
     CDS             2135..3142
                     /locus_tag="PKZ52_08455"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNG59844.1"
                     /translation="MTATRILTGIKTTGTPHLGNYVGAIRPAIAASHTPNTQVFYFLA
                     DLHALIGCHEPAKILQSTKEIAATWLALGLDTDKAVFYRQSDVPEISELCWVLNCMAA
                     KGLMNRAHAYKAAVAQNLEDQQDADFGVTMGLYSYPVLMAADILMFGAHKVPVGRDQI
                     QHVEMARDIAQRFNHHFGETFVLPEAVVDDKVAVLQGLDGRKMSKSYNNTIPLFLAEK
                     PLQKHINKIVTNLLEPGEPKDPDSSAVFHIWQAFASSEQITYMREQFAAGIGWGEAKK
                     QLAALINAELAPARERYETLLANPQLIEAELQKGAAKAREYAIPLMQKVKRAMGIAPL
                     C"
     gene            3149..4411
                     /locus_tag="PKZ52_08460"
     CDS             3149..4411
                     /locus_tag="PKZ52_08460"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF006964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HNG59845.1"
                     /translation="MQPKLRLSAWGDKIAGPSAIVDLMEDLGAALNNNPDLLFLGGGN
                     PAQIPAVQAIFNRHWQALGEDLAAVNKVCGVYQSPQGDEQLLAQLLDLFKQQGLDIAA
                     NNLAIISGSQNALFLLFNLLAGQDGSGQVRKILLPMVPEYLGYADQSLAAESFISCLP
                     QINLTAPHRFRYQLNFAALDKISAPLGALCVSSPTNPSGNVLSLEDLQQLNTWAKARQ
                     IPLIIDRAYGSPFPALEYPPQPLFFDDNTVQVWSLSKLGLPGLRTAVVLARPDIIALL
                     VKANTILNLANNNPGPAIMERLIRSGDLTQITQNLLPDFYRQQRDTLMQLLDEALAGL
                     NYFICEPKGAFFIWLWLPDLPISSHALYELLKQQGVLVMAGEGFFFEWQHTWPHARQC
                     LRLTYCQNKEVLAQAVAILAAQVRRLSI"
     gene            complement(4413..>6790)
                     /locus_tag="PKZ52_08465"
     CDS             complement(4413..>6790)
                     /locus_tag="PKZ52_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59846.1"
                     /translation="GLNLTEWLLDKLTHNLFCSCHEPEYACSCRVGISKIVGTLLDHY
                     FSLAEPPFYPVRVWQWVNNLHFRTSKNKNQSAAVKTLQENQFLRQGIIAHALGEITDK
                     QQINDTYRSKFSGSHTHSGLILNTDDFLFIVDLAFQTNNLVLWTDFIAYHYRYSAGGT
                     NTQRRKMRQQALKNPIFMQAWLSLNRAHQEKLKEYRHQNTLRIRRTYRKEKNARLNKI
                     KYIQEKIATIETGQNFDYLLQLARFTLFHPQDIEIEFGDEALVRRALRNCLNFLTPLA
                     PDLPTLAELHCTSKTLDQELVIYAACLENFRANGNLNNVALPLLKSLRIIVNVGGLTG
                     VSEEDRNGLREEIDHIIFPDTEAIEQFLREYIEPQLTHINCSHANLGLLLNDNTFSNL
                     RATLSIDWLKRFPDLALEPLNKLFEAAAEYGNRNQLKTIIDQHCSEFMSKWPTATNND
                     STEQKRQFWLLRAWCFLDETPDEYWNWIAADKNTLLTLDRFYGQLNRGYYNHWPNLTS
                     TKIEAILNAFIDKWPKVDLPCHFGAGRPAEERAYRFLTNIIWLTNKDTSKKVVTVLER
                     LIANPRLIDSRNDLKSILAEQIRKRALQNFRPPPPDAIVQLLDHNEAVTVEGLRQQVL
                     QELQALQGAIDGGEYNTTELFYADNRRKSEEQCRDRIAEQLQLRLKNYNITITLEHQF
                     KDAKRCDFAATKTIDSKKLLLATEVKGQWHKDLYTAPTAQLLECYASHTDAEQQGIFL
                     VIWYGPHEKVANRKLHGIASAQELKQRIEEKLPNELRGRIDVFVLDVSRHV"
BASE COUNT         1576 a         1696 c         1716 g         1802 t
ORIGIN      
        1 ccgtgaccta tccatcgccg ggcgagtggc tgccaaaacg gctagcggcg ccattattca
       61 ccccttggtg gatttcaaac ggcccatcgc agtgatcccc agcctcgcta ttcacctgga
      121 ccgcagcgct aacgagcagc gctcggtaaa cccgcaagag gatatgcggc ccattctgtt
      181 gcaggatggc agcttggatt ttcgcgctct gctgctcacc cagttacaag cccaggggca
      241 caaggatgtg agcgaaattt ttgagtacga cctgtgccta tacgacagcc aacccgctgc
      301 cctcgtgggg gtgcaggagg agtttatcgc cagcgcccgc ttggataatt tactcagttg
      361 ttatctgggc ctgcaggcaa tgctcagttc tgccggggag cagtcggccc tgctgttgtt
      421 gaccgaccat gaagaggtgg gtagccagtc ggatgtgggt gctcgcagca acctgttaaa
      481 cggttttttg cagcggctct ttagcagtga tgaagagcgt ttgcgagcac ttgctaattc
      541 tatgatgctg tcggtggata acgcccacgg cattcaccca aactatcccc gcaagcacga
      601 tgacaaccac ggcccgctgc tcaacaaagg cccggtgcta aaagtggacg ccaaccaagg
      661 ctacgccacc agcagcgaat ccgccgccct ggtgcgcctg ttagcccagc aggcacaggt
      721 gcccattcag tcttttgtca cccgcgccga tatgcgctgc ggcagcacca tcggccccat
      781 gacggctgcc ctaacgggcg tgcgcacggc ggatctgggg gtgcccacct tcgccatgca
      841 ctccatccgc gagttggcgg gcgcccgtga cccagacctg atgctggcgc ttctatccgc
      901 ctttgtggcg gcagagcagt taagcggctg aggctgaaaa tgttaacctg gttatgattt
      961 tgcttttcca aagctccaat gagttttatt tggcagcctt catccaaaag tgcgctttac
     1021 ttgttaaagc cgaggttatt ttatgatcac agatgatgta ttggctcgct ttgagcactt
     1081 tgacaaactc accccccacc tgctgctgga cgccctcgat gccctggagt ttttgcgtat
     1141 cgacgaaggc ggcttcctgt ttaaacgcgg taaatccctg cctgaagcct actacctgct
     1201 cgatggcagc ctggatttaa tcgattccaa cttcaatacc agctcgattt cagcgggcag
     1261 ccccgaagcc ctgtgcgccc tcaacagcga cccggtcacg gcggtaaccg cccgcgccac
     1321 cagccgagta ctggcctttg ccatcaaccg cgaacaccta tcccgcctgg ttacctacag
     1381 tcaatccagc ccgcaactct ccagcgagcg cgccccaggc gctgatgcca tcgaattaat
     1441 ggaggatgac gactggttcg agcgcctgct ggactcccat ttgttctccc gtattcccat
     1501 ggcccatgtg cagcagttgt tcacccgcct gcgctccatc ccggttaaag ccggtgaaaa
     1561 aattatgcgc gaaggggagc ggggggatta tttttatgta ctcgccagcg gcaatgcggt
     1621 tattaccaat gccctgggca ccatccgggt ggacctaacc cccggcatgt ccttcggtga
     1681 agaggccatt ctgggtaaaa ccccccgcaa tgccacggtt accatgctca ccgatggggt
     1741 gattaaacgc ctcactgaag aggactttaa cgagctgatt cgccgcccgg tgatgcaata
     1801 catcgaggcc tcagcggtgg aagcgctctc tgaatccttc acggtgctgg atgtaaaaat
     1861 gccgatcgaa tttcgctgcg gccactaccc cggcgccatc aatatgcccc tggcccgcct
     1921 gcgcgatcag ttaaaagacc tcgccaccag caagctgtat ttggtgccgg acgacgctga
     1981 agggcgagcg gaaattgccg cccatattct ctgccaggcc ggttttaatg tgcgcattat
     2041 taagggcgcg gcagaggtta tttatggcga gcgggccggt tgaaacatca ggctaacctt
     2101 gtatgatggc cgccttacaa aagtaggcgg ccctatgaca gcaacgcgta ttctcaccgg
     2161 cattaaaacc accggcactc ctcacctggg taattatgtg ggcgctattc gcccggccat
     2221 tgccgcaagc cacacgccta atacccaggt attttatttc ctcgccgatt tacacgcctt
     2281 aatcggctgc cacgaacccg ccaaaatttt gcaatccacc aaagaaattg ccgccacctg
     2341 gttggccctg ggcctggata ccgacaaggc tgtgttttac cgccaatcgg atgtgccgga
     2401 aatttccgag ctgtgttggg tgctcaattg tatggccgcc aaaggcctca tgaatcgcgc
     2461 ccatgcctat aaagcggcgg tggcgcaaaa cctggaagac cagcaggatg cggattttgg
     2521 tgtaaccatg gggctgtatt cctacccggt attaatggcg gcggatattt taatgttcgg
     2581 cgcccacaag gtgccggtgg ggcgcgatca aattcaacat gtggaaatgg cccgcgatat
     2641 cgcccagcgt tttaaccacc attttggtga aacctttgtg ctgcccgaag cggtggtgga
     2701 cgataaggtt gccgtattac agggcctgga tggccgtaaa atgagcaaga gttacaacaa
     2761 tactattccg ctgtttttag ccgaaaaacc cctgcaaaaa cacattaata aaattgtcac
     2821 caatttatta gagcccggcg aacctaaaga cccagatagc tctgcggtat tccacatttg
     2881 gcaggctttt gccagcagcg aacaaatcac ctatatgcgc gagcaatttg ccgccggtat
     2941 cggttggggc gaagcgaaaa aacaattagc cgcgttaatt aacgccgaat tagcccccgc
     3001 tcgtgagcgc tatgaaaccc tgctggccaa cccgcaatta attgaagccg aattgcaaaa
     3061 aggcgctgct aaagcgcgcg aatacgccat tcctttaatg caaaaagtta aacgcgccat
     3121 gggcatcgcg cccctgtgtt aaatctttat gcagcctaaa ttacgtttaa gcgcctgggg
     3181 tgacaaaatc gccggcccca gcgctattgt ggatttaatg gaggatctag gcgcggcgct
     3241 gaataacaac ccggatttat tatttttagg cggcggcaac ccagcgcaaa ttcccgccgt
     3301 gcaggccata ttcaaccgcc actggcaggc cttgggggag gatttagcgg cggtcaataa
     3361 agtctgcggc gtttatcaat ccccccaggg ggatgaacaa ttgctagccc agctgttgga
     3421 tttatttaag cagcaggggc tggatatcgc cgctaataac ctggccatta tcagcggcag
     3481 ccaaaacgcg ctgtttttat tatttaattt attggcgggt caagatggca gcggccaagt
     3541 gcgcaaaata ctgctgccca tggtgccgga atatttgggt tatgccgatc aaagcctcgc
     3601 cgctgagagt tttattagct gcctgccgca gattaacctc accgcccccc acaggtttcg
     3661 ctatcaatta aattttgccg ccctggataa aatcagcgct cccttaggtg cgctgtgtgt
     3721 ttcctccccc accaatccat cgggcaatgt attaagcctt gaggatttac agcaattaaa
     3781 tacttgggcc aaagcgcggc aaatcccttt aattattgac cgcgcctatg gctcaccgtt
     3841 tccggcgcta gaatatccgc cccagccgct attttttgat gacaacaccg ttcaggtgtg
     3901 gagcttgtct aaattaggct taccaggttt gcgcacagcg gtggtattag ccaggccgga
     3961 tattattgcc ctgctggtaa aagctaacac cattttaaac ctggccaata ataaccccgg
     4021 ccccgcaatc atggagcggc tcattcgcag cggggattta acccagatta cacaaaacct
     4081 attgccagat ttttaccgcc agcagcgcga taccctgatg cagttattgg atgaagcctt
     4141 ggcgggttta aattatttta tttgcgagcc taaaggcgcg ttttttattt ggttatggct
     4201 gccagatttg cccattagca gccatgcgct atacgaatta ttaaaacagc agggcgtgtt
     4261 ggtaatggca ggggaggggt tcttttttga atggcaacac acctggcccc acgcccgtca
     4321 gtgcctgcgc ctaacctact gccagaacaa agaagtatta gcgcaggcag tggcgatttt
     4381 ggcggcgcag gtgcgcaggc taagtattta attcaaacgt gcctagatac gtcgagtacg
     4441 aatacatcaa ttcggcctct aagctcattt ggtagttttt cctcaatacg ctgttttaat
     4501 tcttgagcac ttgcaatccc gtgcagttta cggttcgcaa ctttttcatg cgggccatac
     4561 caaataacga ggaaaatacc ctgctgctcg gcatctgtat ggctagcata acattcaaga
     4621 agctgggcgg ttggagctgt gtataaatcc ttatgccact gccctttaac ttctgttgcg
     4681 agtaagagct ttttgctgtc aattgtttta gtggcagcaa aatcgcatcg tttggcgtct
     4741 ttaaattgat gttcgagtgt aattgtgatg ttgtaatttt taagccgtag ttgtaactgc
     4801 tcggcaattc tgtctctgca ttgttcttcg cttttgcggc ggttatcggc gtagaaaagt
     4861 tcagtcgtgt tgtattctcc gccatctatc gcaccctgta acgcttgcag ttcttgtaga
     4921 acttgttggc gcaatccttc tacggttact gcttcattat gatccagtag ttgtacgatg
     4981 gcatctgggg gcggcggtcg aaaattctgt aaagctcttt tccttatttg ttctgcgaga
     5041 atgcttttta agtcattgcg cgaatctatg agacgagggt tggcaatgag ccgctcaaga
     5101 acggttacga cttttttaga agtgtcttta tttgttagcc agataatatt ggtaagaaaa
     5161 cgataagctc tttcttctgc ggggcgtcct gccccaaagt gacagggtaa atctactttg
     5221 ggccatttgt cgataaacgc gtttaaaatg gcctctattt tggttgaggt gaggtttggc
     5281 cagtggttgt aatagccgcg gttaagttgt ccataaaatc gatctagtgt caaaagtgta
     5341 tttttatccg ccgcaatcca gttccagtat tcatctggtg tttcatcaag gaaacaccag
     5401 gcgcgcagca accaaaattg ccgtttttgc tcagtggagt cattattggt tgctgttggc
     5461 cattttgaca taaattcact gcagtgctgg tcaataatgg tttttagctg atttctattg
     5521 ccgtattctg ctgccgcttc aaaaagttta tttagtggct ctaaagctag gtctggaaag
     5581 cgttttagcc agtctataga taaggtggct cgcaagttgc taaaggtatt gtcatttagt
     5641 agcaggccaa ggttggcatg gctgcagtta atgtgggtta gctgtggctc aatatattct
     5701 ctgaggaatt gctcaatagc ttcggtatca ggaaatatta tgtgatcaat ctcctctctt
     5761 agcccattgc gatcctcctc agaaacgccc gtgagaccac ccacgttgac aatgatgcgc
     5821 agggacttta agagtggtag ggcaacattg ttcagattgc cattggctcg aaaattttcc
     5881 aaacaggcgg cgtagataac taattcttgg tctaatgttt tagaggtgca atgtagttcg
     5941 gcaagggtag gtagatcggg cgcgagaggt gtaagaaagt tcaggcagtt tctgagcgct
     6001 ctgcggacga gtgcttcatc gccaaattcg atctcaatat cttgtgggtg gaaaagtgta
     6061 aatcttgcaa gttgcaacaa gtagtcaaag ttttggccgg tttctattgt ggcaattttt
     6121 tcttgaatat atttaatttt attaagcctg gcatttttct cttttctgta ggtgcgtctg
     6181 attctgagtg tattttggtg tctgtactcc ttaagttttt cctggtgcgc cctgttgagc
     6241 gataaccatg cctgcataaa aataggattt ttaagtgcct gttgacgcat tttgcgccgc
     6301 tgggtgttcg tacctcctgc gctatagcga tagtggtaag caataaaatc agtccacagg
     6361 acgaggttgt ttgtttggaa tgctaggtca actataaaca aaaaatcatc agtattcagt
     6421 atgaggccgg aatgggtgtg agaccctgaa aacttagatc tataggtatc attaatttgt
     6481 tgtttgtcag taatttctcc aagggcgtga gcaattatac cttggcgcaa aaattggttt
     6541 tcttgtaatg tttttacggc tgcgctctga tttttgtttt tgctcgtgcg gaaatgcaaa
     6601 ttattcaccc attgccaaac ccgtactggg taaaatggtg gttcggctaa tgagaagtag
     6661 tggtctaaaa gtgttccaac aatcttgcta ataccaactc tgcagctgca cgcgtattcg
     6721 ggttcatggc aggagcaaaa gaggttgtgg gttaatttat ctaacagcca ttcggttaga
     6781 tttaggccaa
//
LOCUS       DAOQGS010000184         6755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5533_length_6755_cov_4.557313, whole genome shotgun sequence.
ACCESSION   DAOQGS010000184 DAOQGS010000000
VERSION     DAOQGS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5533_length_6755_cov_4.557313"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..513)
                     /locus_tag="PKZ52_08470"
     CDS             complement(31..513)
                     /locus_tag="PKZ52_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59847.1"
                     /translation="MRKTYIINYASDALEAIADLESHFCNASDNTLQDYYKIGERLIE
                     RKNIDQTIVDVLITAKTSNTFYRWLNGTRFYLCDKIRRSLELYELSSDVNYCESAYEL
                     FTDLQNIANFDVSDFEFEFIKKKSKKKALHKLPNKWREQLINYNPNSKYRMAFLTAAL
                     "
     gene            complement(734..1027)
                     /locus_tag="PKZ52_08475"
     CDS             complement(734..1027)
                     /locus_tag="PKZ52_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59848.1"
                     /translation="MTNIAFPQNSSRRFFSFIYPLPPRLFIQFYFPLPSTNSATQFIF
                     NFGALKNQQLGKPEVKNALRHFFNFSEEPTIKKIFLGFTHRKNHFFDIDLEFF"
     gene            1243..1446
                     /locus_tag="PKZ52_08480"
     CDS             1243..1446
                     /locus_tag="PKZ52_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59849.1"
                     /translation="MGRLDKVCTPFLQEQGRAGGRQGAAKKLPVRLKLTTALSSLRFL
                     GQSIFHQSIHRNGQDSPTLIVHP"
     gene            1617..3854
                     /locus_tag="PKZ52_08485"
     CDS             1617..3854
                     /locus_tag="PKZ52_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNG59850.1"
                     /translation="MTALSGISEHFGIRAAIADPHNDVSNVMQGANCCYCPEGYCVLV
                     NLFGKYKTLVLSIALFLLLDASVLAVNFYTSYQIALDAKTVNLAGRQRMLSQRTVKAL
                     FEVEKAIAKETSPQVYLNEVAQAAQMFGQTLSAFEQGGAAPGVQDTLVLLAPVKPGAG
                     REALMAAKEIWQVYAQLIDQLLKQKTPEEIKPALAIAMDYARANNLTLLNLMNTLTLD
                     LERAASAKAHQLRLIQTVAIVLAVANFILILWHFLGQLRRSDQALDAARKETQQILST
                     VNEGLFLLDNRLRMGSQHSAKLVQMFGAQADGAGLEQCDFSDFLRDRVRPTDLETALR
                     FVNLLFRRNIKTSLIGDLNPLTQVEINVADECGGYTSKYMHFDFRRVVEEGEIKQVLV
                     TVKDITKEVVLSKELDATRQQNAQQIEMLTGILHANPYVLKLFIQNAFDAFKRINELF
                     RAQDKSSAAMERKLRDIFIEVHTFKAEAGSLHLHTVVNLAHELETSIQTVLAQPIVAG
                     TDLFAAIVLLEKLIYYIEGMQNLTEKVARLAAEAEPVAVPRPKKSANRWQHLQTLAQV
                     LAARYEKKLVLHLTGFNEIQLSPEQARLINDMCLQFIRNAVVHGIEKPQMRLICNKPE
                     VGRIDLSLIRLADGGIELNVRDDGRGVDYDKIRNQAIFMGLAKADELRSWNNKQIIGL
                     MFKPGFTTADELSGDAGRGIGMDVIVSRVQRLKGSIKIASRSGQACQFVVTLPGDDGV
                     KSQAA"
     gene            3862..4257
                     /locus_tag="PKZ52_08490"
     CDS             3862..4257
                     /locus_tag="PKZ52_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG59851.1"
                     /translation="MMPFNRPKRIYSMLVVDDSNVIRRKIERAAEGAFFRVVATAGSG
                     EEALLAFNQHRPELITMDITMPQMDGIACIERLMAKDSEVRILVISALADKATGIEAL
                     EKGARGFLCKPFTEKQLNDALCELVEDSL"
     gene            4254..4703
                     /locus_tag="PKZ52_08495"
     CDS             4254..4703
                     /locus_tag="PKZ52_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNG59852.1"
                     /translation="MTEEALHVFVEGVVNYFRHTADKGVKVGSPYLAETVEPVMHDFT
                     GIIGVSGPYRGSVYFSAPRLLLTHLLLALGEVDTSAENILDLVGEVANTISGNARKTF
                     GREFLISVPVMIEGAVDKIYTPKHTRCFVIPVFWKSYGATVVVCLEH"
     gene            4715..5251
                     /locus_tag="PKZ52_08500"
     CDS             4715..5251
                     /locus_tag="PKZ52_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59853.1"
                     /translation="MAAFAPIMTHEIPAPPASPEAFYAFIDTCLAELEAKQATLLARY
                     NMGRQARWWFDPATNQLALYNDPKAKIDLRAQVVVLGSFAPEESTWLWGFANKTLQPA
                     QQAASQPVQALAERFGVEDFKQSEAFEIDPGMAWELAAMACHSLDLLGVYRTSGSLNQ
                     HQWFLGLISIEENLPPTQ"
     gene            5339..>6755
                     /locus_tag="PKZ52_08505"
     CDS             5339..>6755
                     /locus_tag="PKZ52_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HNG59854.1"
                     /translation="MFFVDSRAQFFRPLTTKYRAQVAECLCLLHQRLYGAAAEYGQSL
                     NRDQVLDIFEEALARSPVLEGEESEQEQRFKSLREQARWILQILLENGWLDKHVDPAN
                     LQTTYPFTRSGRLFAAPLVEAERRASRTRHRNTRNTLNALEAFLSRGEIHDLLDAWDF
                     SERIISDFTDVISELEERKRELVREAQTQLLVQQATEQFFDFMEKRFQPDLSVRLTAD
                     SVEKHRDRIASVLGKIRRKDKTFKQEAERKLRHVLPELIDHHASILWHLLDTIEQRMH
                     NAAEIMLPALRQALQGFTKRADIIIRQLGYLTSSRDDKWVRTCDVLLKLPADECARRL
                     ELAGNAMASAQLGLVDPAQIKLGQKRRERSVSTFIEEPERLDSAAHRELMVQSLLDQA
                     FLINNKTLRHYIGEALKETNSIHTKDLPIDSAQDLLNLAYIIELASTTNQSSDYRFVV
                     KYNQQTYSNAYFDRADDFTLSL"
BASE COUNT         1716 a         1548 c         1702 g         1789 t
ORIGIN      
        1 tagatcaatg tccacttttg gtggtaggga tcatagcgca gcagtgagaa aagccattct
       61 gtattttgaa tttgggttgt aattaataag ttgttccctc catttgtttg gtagtttgtg
      121 aagtgctttt ttctttgatt ttttctttat gaattcaaat tcgaagtctg atacatcgaa
      181 gttagcgatg ttttgtaaat cagtgaaaag ctcatatgca ctttcacaat aattaacatc
      241 tgaagacaat tcgtaaagtt ccaaactccg tctaatttta tcgcataaat agaatcttgt
      301 tccatttaac cacctgtaaa atgtgttgct tgtttttgct gtgatgagta catctactat
      361 tgtttgatct atgttttttc tttcgattaa tcgctcgcca attttgtaat aatcttgaag
      421 agtgttatca cttgcattgc agaagtgcga ctcaagatct gcaatggcct caagcgcatc
      481 agatgcatag ttaattatgt aagtctttct cattgttgct cttcgatttc aattcaatcc
      541 aatcgttgat ttccatctca ttaaaagcac ttataccagc agttaacgcg attggctttg
      601 gaaagctttt atctgaattc atgattcgat agagagttgg tcgactgatt gaaagctttt
      661 tgcatagctg atttattcgc agtagattca tttttcacct tcaacgaaga atgaggaatg
      721 gatagataat gattcaaaaa aattctaaat caatgtcaaa aaaatgattt ttcctatgtg
      781 taaaaccaag aaatattttt ttgatagttg gttcttcgga aaagttaaaa aaatgtcgta
      841 atgcattttt aacttctggt ttgccaagtt gttgattttt aagtgcgcca aaattaaata
      901 tgaattgagt agctgaattt gttgaaggta atgggaaata aaattgaata aacaggcggg
      961 gagggagggg atagataaaa ctgaaaaatc gtcttgatga attttgtgga aatgcgatat
     1021 ttgtcacaaa tttatttgat gcaaaaaaag tataggtaga aatgcaggtc gattagggtt
     1081 tgttagagaa agtccttatt aatcaatcgc ccgataccca tgcatcatgg gggtgtggag
     1141 ttctgaggcg gaatcgagca tggccatatc aatcagcgtt gcgaaaaagg cgcgcagttt
     1201 aacctgaaat tccccgccgc acgcaggtgg gcggcggcag ctatgggccg tcttgataag
     1261 gtatgcacac cttttttgca ggagcaaggc cgcgctggag ggaggcaagg ggcggcaaaa
     1321 aaactgccgg tacgtctaaa gctaacaacc gctttaagtt cactgcggtt tttaggtcaa
     1381 agcatttttc atcagagcat tcatcgcaat ggacaggatt ctccaacatt aatcgtccac
     1441 ccataatttt gcctgcaaag agagcatgtc gagcacagtt ttgcgccatt taattgcccc
     1501 gcagtaatta attgaggttc gacttagttc gcgccaacgt aaagggcgca tttaaagtgc
     1561 gggcttgggg tgtgtgaacg gcatttttgc cgttaattaa ttattcctgg cgccaaatga
     1621 cggcactgag cgggatttca gaacactttg gcataagagc tgctattgca gacccgcaca
     1681 atgatgtgtc gaacgtgatg caaggcgcaa actgttgtta ttgccctgag ggttattgtg
     1741 tgctggtgaa tttattcggc aaatataaaa cgctggttct ttctattgct ttgtttctac
     1801 ttttagatgc ttccgttctg gcggttaatt tttacacctc gtaccaaatt gccttggatg
     1861 ccaaaaccgt taatttagcc ggccgccagc gcatgttgtc ccagcgcacg gtaaaagcct
     1921 tatttgaggt agaaaaagcc atagctaagg agacttcccc gcaggtttat ttaaatgaag
     1981 tggcgcaagc ggcgcagatg ttcggccaaa ccctgagcgc atttgaacag gggggcgcag
     2041 cccccggtgt gcaagatact cttgtgttgc tcgcgccagt taaacccggc gctggtcgcg
     2101 aagctttaat ggccgctaaa gaaatttggc aggtgtatgc gcagctgatc gatcaattgt
     2161 taaaacaaaa aacgccggag gaaattaaac cggctttggc catagccatg gattacgccc
     2221 gcgccaataa tcttaccctg ttgaatttaa tgaataccct caccctggat ttagagcgcg
     2281 ccgccagtgc caaggcccac caattgcgcc tgattcaaac cgttgccatt gtgctggcgg
     2341 tggctaattt tattcttatt ttatggcact ttttaggcca gttgcgccgc agcgaccaag
     2401 cgctggatgc cgctcgcaaa gagacccagc agattttaag tacagtgaat gaaggcttgt
     2461 ttttgctgga taatcgcttg cgtatgggtt ctcagcactc ggctaaactt gtgcaaatgt
     2521 tcggcgccca ggcggatggc gctgggcttg agcagtgtga ttttagtgat tttttgcgcg
     2581 atcgcgtgcg ccctacggat ttggaaaccg ccctgcgttt tgtgaatttg ctgttccggc
     2641 gcaacattaa aacgagctta attggcgatc ttaatccgct cacccaagtg gaaattaatg
     2701 tggccgatga gtgcggcggt tacaccagta aatacatgca ttttgatttt cgccgggtgg
     2761 tggaggaagg cgagattaaa caggtattgg tgaccgtaaa agatataacc aaggaggtgg
     2821 tattaagcaa agagctggat gccacgcgcc agcaaaatgc ccagcaaata gaaatgctca
     2881 ccggaatatt gcacgccaat ccttatgtat taaagctgtt tatccaaaat gcctttgatg
     2941 cctttaaacg cattaatgag ttgtttcgcg cgcaggataa gtcttccgcc gctatggagc
     3001 gcaagctgcg agatattttt attgaggtgc atacctttaa agccgaagcg ggcagtttgc
     3061 atttgcatac tgtagttaac cttgcccatg aattagagac cagtattcaa accgtattgg
     3121 cccagcccat agtggccggg acggatttat tcgcggctat tgtgttgctg gaaaaactta
     3181 tttattacat tgaaggtatg cagaatctaa ccgaaaaagt ggcccgcctg gccgcagagg
     3241 cagaacctgt tgcggtgccg cggcctaaaa aatctgccaa ccgctggcag catttacaga
     3301 ccctggcgca ggtgttagct gcgcgctatg agaaaaaact ggtgctgcac ttaaccgggt
     3361 ttaatgaaat tcaactcagc ccagaacagg cgcgcctgat taacgatatg tgtttgcagt
     3421 ttattcgcaa cgcggtggtg cacggcatcg aaaaacccca aatgcgttta atctgcaata
     3481 aacctgaggt gggccgtatt gatttaagct tgattcgttt agctgacggc ggcattgagc
     3541 ttaatgtgcg cgacgatggc cgcggcgtgg attacgataa aattcgcaac caagcgattt
     3601 ttatggggct tgccaaggcc gatgaattgc gcagctggaa caataaacaa attatcggct
     3661 tgatgtttaa acctgggttt actaccgcag atgagctcag cggcgatgcc gggcggggca
     3721 taggtatgga tgttatcgtc tcccgcgtac agcgcttaaa aggtagtatt aaaattgcca
     3781 gccgcagcgg ccaagcctgt caatttgtag tgaccctgcc gggggatgac ggcgttaaat
     3841 cccaagcagc ctgatagcac tatgatgcct tttaatagac ccaaacgtat ttattctatg
     3901 ctagtggtgg atgattccaa cgtgattcgc cgcaaaattg agcgcgccgc tgaaggggca
     3961 tttttccgcg tggtggccac tgcaggttcc ggtgaagaag cattattggc ctttaatcag
     4021 catcgcccag aattaattac tatggatatc accatgcccc agatggacgg tatcgcctgt
     4081 atcgaacggc tgatggccaa agacagtgag gtgcgtattt tggttatttc tgctctcgcc
     4141 gataaagcca cgggcattga agccctggaa aaaggcgcgc ggggcttttt atgtaaaccc
     4201 ttcaccgaga aacaactcaa cgacgcgctg tgtgaattag tggaggattc cctatgaccg
     4261 aagaggcact gcacgtattt gttgaaggtg tagtgaatta ttttcgccac acggcagata
     4321 aaggcgtaaa agtaggctcg ccctatttgg ccgaaacagt tgaaccggtt atgcacgatt
     4381 ttaccggtat tattggtgtt tccggcccct atcgcggctc ggtgtatttt tcggcgccgc
     4441 gcctgttgct aacgcattta ttgctggctt tgggcgaggt ggataccagc gctgaaaata
     4501 ttttagattt ggtgggggag gtggccaata ccatttccgg caatgcgcgc aaaacctttg
     4561 ggcgggaatt tttaatatcg gtgccggtga tgattgaggg cgcggttgat aaaatctata
     4621 cccctaaaca cacccgctgt tttgttattc cggtattttg gaaatcctac ggcgccacgg
     4681 tggtggtttg tttggagcat taaagcaggc taccatggcc gcttttgcgc ccattatgac
     4741 ccacgaaatc cccgcaccac cggccagccc ggaggcgttt tacgctttta tcgatacctg
     4801 cctggcggaa ttagaagcca aacaagcaac cttattagcc cgctacaaca tgggccgcca
     4861 ggcccgctgg tggtttgatc cggccaccaa ccagctggcc ctttataacg accctaaagc
     4921 caaaatcgac ctgcgcgccc aagtggttgt gctcggcagc ttcgccccgg aggagagcac
     4981 ttggctgtgg ggttttgcca ataagaccct acaaccggcc caacaagcgg caagtcagcc
     5041 ggtgcaagcg ctggctgagc gctttggggt ggaggatttt aagcagtcgg aagcctttga
     5101 aatagacccg ggcatggcct gggagctggc cgccatggcg tgccatagcc ttgatttgtt
     5161 gggggtatac cgcaccagcg gcagtctcaa ccagcatcaa tggtttttag gccttatcag
     5221 cattgaggaa aatctgcccc ccacccagta gcgcccggaa gaaaaagaag tgaaggccgc
     5281 cgccatctgg ccggttgcct ttatgtataa tgcgccgctt cttaggagcg gccctgtcat
     5341 gttcttcgtc gattcacgcg cccagttttt cagacccctc accaccaaat accgcgccca
     5401 agttgccgag tgtttgtgcc tgctgcacca gcgcctgtac ggcgccgcag ctgagtacgg
     5461 ccagtcttta aaccgcgacc aggtgctgga tatttttgaa gaggccctgg cccgctcccc
     5521 ggtgttagaa ggggaggaga gcgagcagga gcagcgcttt aaaagcctgc gcgaacaggc
     5581 ccgctggata ttacaaattc tgttggaaaa cggttggctg gataaacacg tggatcctgc
     5641 gaacctgcaa actacctatc cgtttacccg ctccggccgt ttatttgccg ccccactggt
     5701 ggaagccgag cggcgcgcct cccgcacccg ccaccgcaac acccgcaata cattaaatgc
     5761 actagaggcc tttttaagcc gcggagaaat ccacgatttg ttggacgcct gggatttttc
     5821 cgagcggatt attagcgatt ttaccgatgt gatcagcgag ttagaggagc gcaagcgcga
     5881 actggtgcgg gaggcccaaa ctcaattatt agtgcaacag gccactgagc agttttttga
     5941 ttttatggaa aaacgctttc agccggattt atccgtgcgc ctcaccgccg acagcgtgga
     6001 aaaacatcgg gaccgtattg cgagtgtttt gggcaaaatc cgccgcaaag ataaaacctt
     6061 taaacaggaa gctgagcgca aactgcgcca tgtattaccc gaattaatcg accatcacgc
     6121 ctcaattttg tggcatctgc tcgacaccat tgagcagcgc atgcacaacg cagcggaaat
     6181 tatgttgccc gccctgcgcc aggcactgca gggttttacc aaacgggcgg atattattat
     6241 tcgccagctg gggtatttaa cctccagccg ggatgataaa tgggtgcgca cctgcgatgt
     6301 gttattaaaa ttaccggcgg atgagtgcgc ccgccgcttg gaattggcag gcaatgccat
     6361 ggcctctgcc caattgggcc tggtggaccc ggcgcagatt aaactcggcc aaaaacgccg
     6421 ggagcgttcg gtgagcacct ttattgaaga gccagagcgc ttggacagcg ccgcccaccg
     6481 cgaattaatg gtgcaatccc tactagatca agccttttta attaataata aaactctgcg
     6541 ccactatata ggcgaggcgt taaaagagac caatagcatc cacaccaaag atctcccgat
     6601 tgattctgcc caggatttat taaatttggc ctacattatt gaattagcct ctaccaccaa
     6661 tcaaagctcg gattatcgct ttgtggtgaa atacaaccaa caaacctatt ccaacgccta
     6721 ttttgatcgg gccgatgatt ttaccctgtc cttgg
//
LOCUS       DAOQGS010000185         6714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5583_length_6714_cov_5.985583, whole genome shotgun sequence.
ACCESSION   DAOQGS010000185 DAOQGS010000000
VERSION     DAOQGS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5583_length_6714_cov_5.985583"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            901..1545
                     /locus_tag="PKZ52_08510"
     CDS             901..1545
                     /locus_tag="PKZ52_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNG59855.1"
                     /translation="MAIKKQFLVLLTCGLCACGGGGDGGGGGTVSVTPPVEVDPKTRA
                     ENETIETLWVSHHHYWDPNDLPAPSMSLIVSPAVAPDFSLEHMQLIPSVTGFTYEPGY
                     VYRIRVKKTKVIHLADLPIYTYTWLATEQKQLATGQVFDFEFQKTTNYRRTGTSIKPL
                     NLPLEFTCDLTLCDAIDAQLSRPEVTSLILAARVQLPGQPIYFTAIKSVVDQAH"
     gene            complement(1665..3314)
                     /locus_tag="PKZ52_08515"
     CDS             complement(1665..3314)
                     /locus_tag="PKZ52_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG59856.1"
                     /translation="MQWLYGLTLKNKLLLPIVIITSLSAALGAITLVQLALIDQHIKL
                     LGRVNLTAANDLLEADSEVHKVLAQERSLLFLNPESAEFIQATQAHAKSLAAAREKLL
                     GFRKAVADSELSAEYDAYSAAADRWEQLTQNVVKARSTNTREGRTTAIEISFKDGNQA
                     FEAMRTHIYALKEIINARSEGLMRASEEAVIHSRYVVGFLLAVCALIAIAMALLIPKF
                     IVGPILNMRQFILRLINEDGDLSQKIPVGFQDELGNLGTTINEFIDSLRELIARVIRL
                     GSVFHQQSKTLKELAQQNSSLVYRETTEIAQVMGSITTLSQSVQNVAQLAIEAANKTA
                     LAQTESNNGLNVVSRTVQGINQLAQNVKNSSDVITQLNSDSANIIGVVNVIRGIADQI
                     NLLALNAAIEAARAGEQGRGFAVVADSVRELAFRTAESTQEIQVMISKLQQSAHQAVE
                     AMTQSQSIAEQSVMQAGETGSALNQIDVSVQRISAVNDQIASSANQQSMVAEHISRNS
                     INISQFAEDGAQLASNVADASDQLERVAAELDKALRKFKTN"
     gene            complement(3323..3907)
                     /locus_tag="PKZ52_08520"
     CDS             complement(3323..3907)
                     /locus_tag="PKZ52_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59857.1"
                     /translation="MLSFKTPITYLKRTVCALFAGACLSLSAQADSGEIIASLIPTIK
                     KWGENPILVAAVKEQNAKKLSLEAIQKRDKEWMATTGLDDQMKALMKNAAANELNQLE
                     ATKPYFFESILMDNQGANVAMTNKTSDYWQGDEDKFTNAYKSGAGGVDIGKSKFDDSA
                     KAYLVQISVPVVDGSSVIGALCVGINLDELEKAK"
     gene            complement(4665..5552)
                     /gene="ispA"
                     /locus_tag="PKZ52_08525"
     CDS             complement(4665..5552)
                     /gene="ispA"
                     /locus_tag="PKZ52_08525"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HNG59858.1"
                     /translation="MSFDLAAYLEARAQEVNQWLKDALPKAIPGSQHLIDAMGYSLLL
                     GGKRLRPILTLAAAHTCGEVTPATRAAAVALEAIHAYSLIHDDLPAMDNDNLRRGHPT
                     CHIAYGEATAILAGDALQTLAFEQLVRADAPGPIRLALIEQLSLAAGSQGMVAGQALD
                     MAATDQSIRLDALELMHRHKTGALIEAAVHMGAISAGETAPERLNALGRYARAIGLAF
                     QVQDDILDVTGDTATLGKQQGADEHLNKSTYVSLLGLDAARQHAKTLVDEALSSIEAF
                     DERAEPLRALARYIIARGF"
     gene            complement(5552..5782)
                     /locus_tag="PKZ52_08530"
     CDS             complement(5552..5782)
                     /locus_tag="PKZ52_08530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNG59859.1"
                     /translation="MAAKKKIDFEKSLSALETLVTAMEKGSMSLDESLKAFEDGIKLS
                     RECQQALQEAEQRVEILLAKGDTAPFDPEETA"
     gene            6015..>6714
                     /locus_tag="PKZ52_08535"
     CDS             6015..>6714
                     /locus_tag="PKZ52_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG59860.1"
                     /translation="MATLIGLVGVTLLLAFAASLSGGLAMFFDVPSLLIVLGGSFFVV
                     MAKFGMSQFLGAGKVASHALQIKALEPQDLIVELVGLADVARRGGLLALEDRRISNPF
                     LKRGVELLVDGQDPELVKALLVKEKQESAQRHQRAAQVFSAFGEVSPAMGMIGTLVGL
                     VSMLGNMSDPKAIGPSMAVALLTTLYGAILANCVFIPLADKLRLRAQEESSCKSLMLD
                     GVLAELQAPESGDAA"
BASE COUNT         1452 a         1583 c         1867 g         1812 t
ORIGIN      
        1 ttggcaaatt caacgacgcc agggcgagta aggtaatttt tcagaggttc cctagcaagg
       61 tagtatttca acaagctgta agttggcctt gtgcttgcga agatgtttga agcctccttt
      121 ttatcgcctt gcatgggatt cttgtaaaag ttaattccct tcttgtgcat gcatggattg
      181 ttaggttgac aggctcaagg taagactttc tgcagggcca aaaactcggt taataatagc
      241 tgcctttaaa agagggggca gctagttcta attgcggctt gctgccaaaa gcgcgaattg
      301 cccgtaatgg agagatgtat aaaaaccact gcgtcaaaaa cctctgtgat agtcccaggc
      361 agatgtatct ttgcttaaca gttacaacta gggaacctct gaaaaattac cttgctcgcc
      421 ctggcggcgt tgaatttggc aaaaaaatgc tcatttactg cgtgtaaact ccgctttttt
      481 tgccaaattc gcctagccag agcttcgccg cctacatttt tcagaggttc cctagcccct
      541 cagacttaat aacgggtgtg cctgttgtgc aggacccatg gcctgtggag tgtagtattt
      601 tcaggcggct agggggtgat aaaggggatt tccaaaattg ttgcgggcag gaaatctatg
      661 tgggaagcac gaatgtgtgt ggtgattctg aatatgccaa ttagcagtct gttgaaacgc
      721 cgtctgctta tttcaacaga ttgtgaagtg gaggttgccc ggtggatcaa gcggcgcctg
      781 gtcgtccaaa tgcggcactg gatctagtcc gcagaatttg cgatccacct ccgaatcgtt
      841 tttgccgctt ggctatatac tgtagcgctg aattgtgtgg tgttacggag tgctcaggtt
      901 atggcaatta aaaagcagtt tctggttttg ttgacatgtg ggttgtgcgc ctgtggaggg
      961 ggtggtgatg gcggcggtgg cggtacggta agtgtcactc cgccggttga agttgaccct
     1021 aaaacacggg ccgaaaatga aaccattgaa accttgtggg tgagccatca ccactattgg
     1081 gaccctaatg atttacctgc acccagtatg agtctgatcg tctcgcccgc tgtagcgcct
     1141 gatttttcgt tagagcacat gcagctgata cccagcgtga caggttttac ttatgagccg
     1201 ggttatgttt atcggattcg cgtaaaaaaa accaaagtga ttcatttggc ggatttacct
     1261 atttacacct atacctggtt ggcaaccgag caaaaacagc ttgctacggg gcaagtattt
     1321 gattttgagt ttcaaaagac aaccaattat cgtcgcacag gcaccagcat taagccgtta
     1381 aatttgccgc ttgaattcac ctgtgattta actttgtgtg atgccattga tgcacagctc
     1441 tcgcggccag aggtgacaag cctgatatta gctgccaggg ttcaattgcc agggcagccc
     1501 atttatttta cggctattaa gtcggtggtt gaccaagccc attaatacac agtgtaccgc
     1561 gggtgttttt ttaaaggagt tgcctgcttt agacggttgc gtaatcttcc gcatgcagac
     1621 ttattaaccc ggcactaatt gccgccgggt caataacctt gctgctagtt agttttaaat
     1681 ttccgcaggg ctttatccaa ttcagcggca acccgttcta attgatcgga agcgtccgcc
     1741 acgttagatg cgagctgggc gccgtcttcg gcaaattgtg agatatttat tgagttgcgg
     1801 cttatatgtt cggcaaccat ggattgttga ttggcagagg aggctatttg gtcattcacg
     1861 gcgctgattc gctgcactga aacgtctatt tgattgagcg cggagcctgt ttctcctgcc
     1921 tgcataacgg actgttcggc aatactttgg ctttgtgtca tggcttctac cgcctggtgg
     1981 gcgctctgtt gtaatttgct gatcatcact tgaatttcct gcgtggactc ggcggtgcga
     2041 aacgccagct cgcgcacaga atcggccacc actgcaaaac ctcttccctg ctcgccagcc
     2101 cgcgccgcct caatcgcggc atttaaggcg agtaaattaa tttgatcggc aataccccga
     2161 atcacattca ctacaccaat aatattggcg ctgtcgctgt tgagctgggt aataacatcc
     2221 gaagagtttt taacattctg tgccagctga ttaatgcctt gcaccgtgcg cgataccaca
     2281 tttaagccat tgttgctttc tgtttgtgcc aaagctgttt tattggcggc ctcaatggcg
     2341 agttgcgcga cgttttgcac agattgggag agtgttgtga tagatcccat cacctgggcg
     2401 atttccgtgg tttccctgta aaccagagaa gaattttgtt gcgctaactc tttcaatgtt
     2461 tttgactgct gatgaaatac cgagcctaag cggattacac gcgcaatcag ttcacgcaag
     2521 ctgtcgatga attcattgat agtggtgccg aggttgccga gttcatcttg aaagccgacg
     2581 ggaatttttt gcgataaatc accgtcttcg ttaattaagc gcaggataaa ttgccgcata
     2641 tttagaatcg ggccgacaat aaatttaggg atgagtagcg ccatggcgat ggcgatcagt
     2701 gcgcacacgg ctaataaaaa ccctactaca tagcggctgt gtattacggc ctcttcagag
     2761 gcgcgcatta agccttcgga gcgcgcatta attatttctt ttagggcgta aatatgcgtg
     2821 cgcatggcct caaaggcctg attgccatct ttaaaactga tttcaatggc cgtagtgcgg
     2881 ccttcgcggg tgttggtgga gcgcgctttt acgacgtttt gggtgagttg ttcccagcgg
     2941 tcagcagcgg cgctataagc atcatattcc gcgcttaatt cgctatcggc tacagccttg
     3001 cgaaagccaa gcagtttttc tcgtgcggcg gcgaggcttt tggcgtgggc ttgagtggcc
     3061 tgtataaatt ccgctgattc ggggttgaga aataataagc tgcgttcctg cgcgagtact
     3121 ttgtgcactt ccgagtccgc ttccagcagg tcgttggcag cggttaaatt aacgcggccg
     3181 agcagtttaa tgtgttggtc tattaaggct aattgcacga gtgtgatagc gcctaaagcg
     3241 gcgctaagac tcgtgataat cacaatcggc aggagcagtt tatttttaag ggtaagtcca
     3301 tataaccact gcataggggg ctctatttgg ctttttcaag ttcatcaaga ttgatgccga
     3361 cgcagagcgc acctattaca ctgctgccgt ccaccaccgg taccgatatt tgcaccaggt
     3421 acgctttggc gctgtcatcg aacttacttt tacctatatc gactccgcct gcaccgcttt
     3481 tataagcgtt ggtaaattta tcttcgtccc cctgccagta gtcggaggtt ttattcgtca
     3541 tggctacatt ggcgccttgg ttatccatga gaatgctttc aaaaaaatag ggtttagtgg
     3601 cttctaactg atttaattcg ttggcggccg catttttcat gagggccttc atttgatcgt
     3661 caagcccggt ggttgccatc cattctttat cgcgtttttg aatagcctcc aggctgagtt
     3721 ttttggcatt ttgctctttg acggcggcaa ccaaaatggg gttttcaccc cattttttaa
     3781 tggtggggat cagcgatgcg attatttcgc cgctatcggc ctgggcgctg aggcttaggc
     3841 aggcgccagc gaatagagcg cagacagtgc gctttagata agttattggg gttttaaaag
     3901 ataacatgct aatgccttgg gttgagggag tgcctagtta ggatacatga gtaatagatg
     3961 ctgggggact gggcgtcaag caaggatagt cgcaggtgtt aacttgggga tcttaacttt
     4021 ggtatgtaaa tggtctagcg aatgtttgct gcgagttatc gcgactaaaa aactaagcgc
     4081 agaagcgccg cttactacct gggcgccagc aatgaataca catcatttca gacacgccgt
     4141 gaatacatcc gtgtaggctc gtcaaaaagg tccctctttt tgacggtctg aaacgaagtg
     4201 tattcattgc ttccgttcag ctaatagaga ttggtgtatt tataaaatgg tttggtaact
     4261 agtcagcacc ttactagtgt ggctggaggt aatttccgca cccactggaa actatcacgc
     4321 gagtgcttga gtacggcgag ccatcgatac tccgctcgcg cttaagaggt taataggctg
     4381 tgctgaatag ttacattatt tgtaagtgcg cataagaatg cccgcttcca aattgaatca
     4441 gcgttgtaca aatagatagg cggagaattt taggtataca caagtaaata ggtggtcaat
     4501 tcaggcgcaa cataaatgcg tgagcgccat tgaatttggc ggcaacagat aaacctctaa
     4561 ggagctgttc cagcgctgta ctgcggtggg tatagcaggg ctgcatcggc acatcggcat
     4621 ggcacatctg caatggttgc cgcagcaact gcggcaaccc agcgttaaaa cccccgcgcg
     4681 atgatataac gagccagcgc tcttaagggt tcggcgcgtt cgtcgaaggc ctcgatgctg
     4741 cttagggctt catctaccag ggttttggcg tgttggcggg cggcgtctag gcccagcagg
     4801 ctcacatagg tggatttatt caggtgttca tcggcgcctt gctgtttgcc gagggttgcc
     4861 gtgtcgccag tgacatccaa aatatcgtcc tgcacctgaa aggccaggcc tatggcgcgg
     4921 gcatagcggc ctaatgcgtt caagcgttca ggtgcggttt ccccagcgct gatggcgccc
     4981 atatgtacag cggcttctat tagggcaccg gttttgtggc ggtgcatgag ctcgagggcg
     5041 tccagtctga tggactggtc ggtggccgcc atatcgaggg cttgcccggc gaccatgcct
     5101 tggctgcctg cggctaagct gagttgctcg atcaaggcca ggcggattgg gccaggtgcg
     5161 tccgctcgca ccagttgctc aaaggctagg gtttgcaggg cgtcaccggc cagaatggcg
     5221 gtggcctcgc cataggcgat gtggcaggtg ggatggcccc ggcgcaggtt gtcgttgtcc
     5281 atggcgggta ggtcgtcgtg gatcaggctg taagcatgga tggcttccag agcgacagcc
     5341 gctgcgcggg tggccggggt tacttcgccg caggtgtgcg ccgccgcgag ggtgagaatc
     5401 ggccgcagcc gtttgccgcc cagtaacagg ctataaccca tggcatcaat caggtgttgg
     5461 ctgccgggta tggccttggg cagcgcgtcc ttaagccact ggttgacctc ttgagcgcgg
     5521 gcctctaggt aggcggctag gtcaaacgac attaggcagt ttcctcaggg tcaaacggcg
     5581 cggtgtcgcc tttggcgagc agaatttcta ctcgctgttc ggcttcctgc agggcctgtt
     5641 ggcattcgcg ggagagttta atgccgtctt caaaggcctt aagggattca tccaaactca
     5701 tggagccttt ttccatggcg gtgacgagtg tttccagcgc actcagggat ttttcaaaat
     5761 cgattttttt cttggctgcc acaagggttt cctcgtcaga agcgaaagcg cgccgcttcg
     5821 ctaaaaggcg gaacactagc ctgccgccgc gccccgctgc aagcccccca ggcataaacc
     5881 taagaacccg aggcgccctt gctgcgagtt caactgaggt ttttaggtta aaactatttg
     5941 gcaggtgaag tcgggttttt atgtctacag ttactacacg ctagatgaac tcgtggctgg
     6001 aggtgcccgt ggatttggca accctcattg ggctggtagg cgttaccttg ttgctggctt
     6061 ttgccgcctc cttaagtggt ggcctggcga tgttttttga tgtgccctcc ctgctgattg
     6121 tgctgggcgg gtcatttttt gtggtgatgg cgaagtttgg catgagccag tttctcggcg
     6181 ctggcaaagt ggccagccac gccctgcaaa tcaaggcgtt agagccccaa gatctgattg
     6241 ttgaactggt gggcctggcc gatgtggcgc gccggggcgg gctgttggcc ttggaggatc
     6301 ggcgtatttc caaccctttt ttaaagcgcg gggtggagct cttggtggac ggccaggatc
     6361 cagaacttgt caaagctcta ctggtgaaag aaaagcagga atccgcccag cgccaccagc
     6421 gcgccgccca ggtgtttagc gcctttggcg aggtttcacc ggctatgggc atgatcggca
     6481 ccctggtggg tttggtgagt atgctgggca atatgtctga ccctaaggct ataggcccct
     6541 ccatggccgt ggcattgtta accactttgt acggcgccat tttggccaac tgtgtgttta
     6601 ttcccctggc ggacaaactg cgattacgag cccaggagga atcctcttgt aaaagtttga
     6661 tgctcgacgg cgtgctggcc gaactgcaag ctcccgagag cggcgacgcg gccc
//
LOCUS       DAOQGS010000186         6696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5609_length_6696_cov_4.415148, whole genome shotgun sequence.
ACCESSION   DAOQGS010000186 DAOQGS010000000
VERSION     DAOQGS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5609_length_6696_cov_4.415148"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKZ52_08540"
     CDS             complement(<1..232)
                     /locus_tag="PKZ52_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG59861.1"
                     /translation="MAKLCYAFDEQTFADFRSQHIQVKPASSPWEKQRYFALRKAVFA
                     QEQKILPDNEQDGQDFRAIAIVALASSCGVVDD"
     gene            complement(225..1337)
                     /locus_tag="PKZ52_08545"
     CDS             complement(225..1337)
                     /locus_tag="PKZ52_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HNG59862.1"
                     /translation="MGLSNQQLTQIQSQGLRWVDADLGLTRSGGAGPTDHKALCFERQ
                     TSMIPVFNQQIQSSPYSAKAENEQVLIFEEDKLIATAAMPGRPKFYDLTTEEGIPYWQ
                     IATLHSKDVLATTVLQTCVRYRNRDTSCQFCAIEESLRNGKTIAHKSPQQLAQVALAA
                     VQLDGVKQMIMTTGTPKGTDRGAQVLVESARAVRSVVDIPIQAQCEPPDDDIWFARLK
                     DAGVDALGMHLEAVSERVRKQIMPGKASVPISRYRSAFKAAVAVFGRGNVSTYILAGL
                     GDTEEEILAMSLELIELGVYPFVVPFVPVAGTPLADHPMPKADMLDRLFKQLGPQLVA
                     AGINSQSLKAGCAKCGACSSLQSYEKQSFESEVAHG"
     gene            complement(1351..2340)
                     /locus_tag="PKZ52_08550"
     CDS             complement(1351..2340)
                     /locus_tag="PKZ52_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitriliase"
                     /protein_id="HNG59863.1"
                     /translation="MSKVVVAAVQCSPVLYSCAGTVDKICGWIAQLGQQGVQMAVFAE
                     TLVPYYPYFSFIQAPCAMGAQHLLLMQESVQVPSIYTAQIAAAAKACKMVVSVGVNER
                     DGGSIYNAQLLFDADGTLVQHRRKITPTFHERMVWGQGDGSGLCAVDTAVGRVGSLAC
                     WEHYNPLARYALMADREQIHVAMFPGSLVGEIFAEQIEVTIRHHALESGCFVVNATGW
                     LSPEQQAQIVADTGGNLAAISGGCFTAIVSPEGKLLGTPLRSDSGEGACVAELDLKLI
                     DKRKRMMDSVGHYSRPELLSLLIDKTPKPQTHLKPQADPEPVNNPLAPSLSAV"
     gene            complement(2377..2859)
                     /locus_tag="PKZ52_08555"
     CDS             complement(2377..2859)
                     /locus_tag="PKZ52_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572 family nitrogen starvation response
                     protein"
                     /protein_id="HNG59864.1"
                     /translation="MPSVNIPHYQDGDFLVDFEEKVFEDVQAEPGAKALLTFHTVAFE
                     GSIGLVNLLQAKRILRKGFDTSVLLYGPGVLLGVQRGFPKLGSEAFPGHLAYNAQLKA
                     FMAEGGKVYACRFALQALYGQTEKALIEGIRPINPLDVMDLQLMMRRDNAFVVHTWTT
                     "
     gene            3130..5037
                     /locus_tag="PKZ52_08560"
     CDS             3130..5037
                     /locus_tag="PKZ52_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNG59865.1"
                     /translation="MFDADTTDDFSKMAYWMRAAAFEQCPEPLMLLDPVQNQYIDCNI
                     AASNLLGFARADILKMPISAIHGRELGELIVFTQEALMGGQAKSKRISCRLASGVHIP
                     VELSGARAVIKGREYLIVCLKDLDTEQRRQNLAEVDRISRNGILEWKHVQSIFQQGER
                     DNLLMLTSVGDGIYCVDTSGMCTFINPAAKRLLGRTDSDVLGQNIHYVHHHTHADGTH
                     YPVEECPIYAAIRDGVVHEGKQEVFWKHDGTPFPVEFTSTPIISAGSIIGAVVVFRDI
                     SQRVQTEQNLRDALQELNELKARLEDENAYLQQEILIEQRYLGIIGESAAIKRIHQQI
                     QLVAQTEACVLITGESGTGKELIARAIHEASNRKDKPMIRVNCAAIPHELFESEFFGH
                     IRGAFTGAVRDRIGRFELANGGTLFLDEVGEIPLDLQSKLLRVLQEGQLERVGEEKTR
                     DVDVRVITATNKNLKKEAEAKRFREDLYFRLNVFPIHSPPLRERGDDIIVLATHFLEQ
                     AGNKLGKKDCKLRKSDLKLLLDYPWPGNIRELQNVMERAVITANQGRITLDLPMVDPK
                     TEAPQIAKPSEQRQVIADDDMHAQVKANMLAALEACNWKLFGEDGAAALLGLKPTTLA
                     SRIKRMGLKKT"
     gene            complement(5709..6278)
                     /locus_tag="PKZ52_08565"
     CDS             complement(5709..6278)
                     /locus_tag="PKZ52_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNG59866.1"
                     /translation="MLKPLIISGHPDLPSSNANQTILTEFAKRHPAAEIRRLDSLYPD
                     YKINIEAEQQALINAGIIILQAPFYWYSVPALLKKWLDDVLAFNFAYGSKGDKLKGKH
                     FIVSLTIGGPKESYNASGYNHFEVEALLLPLKQTALLAGMIYQPPVYSHGMVYIPGIY
                     GDLPSITQQALEQAERLSEQVLRIQSENA"
     gene            6463..>6696
                     /locus_tag="PKZ52_08570"
     CDS             6463..>6696
                     /locus_tag="PKZ52_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG59867.1"
                     /translation="MKSIVFEQLRVFQAIARAGGISAAARALQLSTPAVSQSLKQLER
                     HLGLTLFHRSTRHMELTAAGHLLLQQTLPLTDGL"
BASE COUNT         1656 a         1846 c         1708 g         1486 t
ORIGIN      
        1 cgtcgtccac caccccgcaa ctggaggcga gggccactat ggcgatggcg cgaaagtcct
       61 ggccgtcctg ctcgttgtcc ggcaggattt tttgctcctg cgcgaacact gccttgcgca
      121 gggcaaaata acgctgtttt tcccaggggg aactggccgg tttcacttga atatgttgcg
      181 agcgaaaatc cgcaaaggtc tgctcatcaa aggcgtaaca caacttagcc atgggccacc
      241 tcactttcga aactttgttt ctcatagctt tgcaatgaag aacaggcccc gcatttggcg
      301 caacccgctt tgagtgattg tgagttaatt cccgccgcca caagctgcgg ccccagttgt
      361 ttaaacaggc ggtcgagcat gtccgccttg ggcatggggt gatcggccag tggcgtgccc
      421 gccaccggca cgaagggcac cacaaatggg taaaccccaa gctcgataag ctcgaggctc
      481 atggccagaa tctcctcttc cgtatcccca agcccagcca ggatataggt gctcacatta
      541 ccccggccaa acaccgccac cgccgcctta aatgccgacc tataacggct gatcggcacg
      601 ctggccttgc ccggcataat ctgtttgcgc acccgctcgc tcaccgcttc aaggtgcata
      661 cccaaggcat ctacaccggc atctttaagg cgcgcaaacc agatgtcgtc atccggcggc
      721 tcacactggg cctggatcgg gatgtccacc actgagcgca cggcgcgggc cgattccacc
      781 aacacctggg cgccgcggtc agtgcccttg ggtgtgccgg tggtcatgat catttgttta
      841 acgccatcga gctgcaccgc cgccagcgct acctgggcca gctgttgcgg gcttttatgg
      901 gcgatggttt tgccattgcg cagggattct tcaattgcac aaaactggca ggaagtatcg
      961 cgattgcgat agcgcacaca ggtttgcagc accgtagtgg ccagcacatc tttgctgtgc
     1021 agggtggcga tctgccaata ggggatgccc tcctcagtgg ttaagtcata aaacttcggc
     1081 cgccccggca tggcggcggt ggcgatgagt ttgtcctctt caaaaattaa cacctgctcg
     1141 ttttcggctt tggcgctgta aggcgagctt tgaatctgct ggttaaacac cgggatcatg
     1201 ctggtttggc gttcaaaaca gagggctttg tggtccgtgg ggccagcgcc acctgagcgg
     1261 gtcaggccca ggtcggcatc cacccagcgc agcccctggc tttggatttg ggtaagttgt
     1321 tggttgctca ggcccatagg atcactccaa ctaaacagcc gacaggctag gggctagggg
     1381 gttgttcaca ggctcgggat cagcctgcgg cttaaggtgc gtctgcggct tgggggtttt
     1441 atcgatcaat aaactcagca gctccgggcg gctgtagtgg cccaccgaat ccatcatgcg
     1501 cttgcgttta tcaataagtt tgagatccag ctcggccacg caggcgcctt cgccactgtc
     1561 gctgcgcagg ggcgtgccga gtaatttgcc ctccggagag acaatcgcgg tgaagcagcc
     1621 gccgctgatg gcagcgaggt tgccgcctgt gtccgccact atctgcgcct gctgctcggg
     1681 gctgagccag ccggtggcat tcaccacaaa acagccggac tccaaggcgt ggtggcggat
     1741 ggtgacttca atttgttcgg caaaaatctc cccaaccagt gagcctggga acatggccac
     1801 gtggatctgc tcgcgatcgg ccattagggc gtagcgcgcc agggggttgt agtgctccca
     1861 gcaggcgagc gaacccaccc ggccaacggc tgtatccacc gcacacaggc ccgagccatc
     1921 gccctgcccc cacaccatgc gctcgtggaa ggtcggagtg attttgcgcc ggtgctgcac
     1981 cagggtgcca tcggcgtcaa ataacaactg ggcgttgtag atggagccgc cgtcacgctc
     2041 attaacaccc accgacacca ccatcttgca agccttggcg gcggcggcaa tttgcgcggt
     2101 gtaaatggac ggcacctgca ccgactcctg catcaacagc agatgttgcg cgcccatggc
     2161 acagggcgcc tgaataaagg aaaaataggg gtaataaggc actagggtct ctgcgaacac
     2221 cgccatctgc acgccctgtt ggcccaactg ggcgatccag ccgcaaatct tatccaccgt
     2281 accggcgcag gaatacaaca ccgggctgca ctgcacggct gccaccacaa ctttactcat
     2341 aaaaaccccc gtaaaaccta agaaaaaacc gctgttttag gtggtccagg tatgcacaac
     2401 aaaggcatta tcccgccgca tcattaattg caggtccatc acatctaggg gattgatcgg
     2461 tcgaatacct tcaattagcg ctttttcggt ctggccgtat agcgcttgca gggcaaaccg
     2521 gcaggcatac accttgccgc cctcggccat aaaggctttg agctgggcgt tgtaggctaa
     2581 gtggccaggg aaggcctcgg agccgagctt aggaaagccc cgctgcacac ccagcaaaac
     2641 acccggcccg tacagcaaca cggaggtgtc aaaacccttg cgcaaaatgc gcttggcctg
     2701 cagcagattt accaaaccga tggagccttc aaaggccacc gtgtggaagg ttaaaagggc
     2761 tttagcacct ggctcagcct gtacatcttc aaaaaccttt tcttcaaaat cgaccagaaa
     2821 gtcgccgtct tggtagtggg gaatgtttac gctgggcatg ggcttacctc tttttaagtg
     2881 gagtgttgtt aggtgcgctc agatcaattg agcggttctc gaactattgc ggcttttgta
     2941 ccagcggccg ccaaatccca ccccaggcgg caaataaaat taaagtcttt taaaaccagt
     3001 tagttagagc gacgtcccca ccgcgagccg aaccagcggc gcagctaaca gagattttta
     3061 aactacgcat ttgcgttaaa ttacgcacct gcgtagcatg ggtctacgca cctgagtagg
     3121 aagcgaatta tgttcgacgc cgataccacc gatgacttca gcaaaatggc ctactggatg
     3181 cgcgccgccg ccttcgagca gtgcccagag ccattaatgc tcctggaccc ggtgcagaac
     3241 caatacatcg actgcaacat cgccgccagc aacctgctgg gttttgcccg cgcggacata
     3301 cttaaaatgc ccatctccgc cattcatggc cgcgaattgg gggaattaat tgtgtttacc
     3361 caagaggcct taatgggcgg ccaggccaaa agcaaacgca tttcctgccg ccttgccagc
     3421 ggtgtgcata ttccggtgga attaagcggt gcccgggcgg taataaaagg ccgcgaatat
     3481 ttaattgtgt gtttaaaaga tttagatacc gaacagcgcc ggcagaacct cgcggaagtg
     3541 gaccgcatca gccgcaacgg catattagag tggaaacacg tgcaatcgat tttccagcag
     3601 ggtgaacggg ataatttatt aatgctcacc tcggtaggcg atggcattta ttgtgtggat
     3661 accagcggta tgtgcacatt tatcaaccct gccgccaagc gtttattagg ccgcaccgat
     3721 tccgacgtat tgggccaaaa tattcattat gtacaccacc acacccacgc tgacggcacc
     3781 cactacccgg tggaagagtg ccccatttac gcggcaattc gcgatggcgt agtccatgag
     3841 ggcaaacagg aagtattttg gaaacacgac ggcacaccct tcccggtgga atttaccagc
     3901 acaccgataa tttccgccgg aagtattatc ggtgcggtag tggtgtttcg cgatattagc
     3961 cagcgtgtgc aaaccgaaca aaacctacgc gatgccctgc aggaattaaa tgaattaaaa
     4021 gcccgcctag aagatgaaaa cgcctattta caacaggaaa ttttaattga gcagcgctac
     4081 ctcggcatca taggcgagag cgccgccata aaacgcattc accaacaaat tcaattggtg
     4141 gcgcaaacag aagcctgtgt attaattacc ggcgaatccg gcaccggcaa agaattaatc
     4201 gcccgcgcca ttcacgaggc cagcaaccgc aaagataaac cgatgattcg cgtcaactgc
     4261 gcggccattc cccacgaatt atttgaaagt gaatttttcg gccacatccg cggtgccttc
     4321 actggcgccg tgcgcgatcg catcgggcgc tttgaattag ccaacggcgg caccttattt
     4381 ttagatgaag taggcgagat tcccttggat ttacagagta aattattgcg tgtcttacaa
     4441 gagggacaat tggagcgggt gggcgaggaa aaaacccgcg atgtagatgt acgggtaatt
     4501 accgccacca ataaaaacct aaaaaaagaa gccgaggcca aacgctttcg cgaagacttg
     4561 tattttcgcc tcaatgtatt tcccatccac tccccgcccc tgcgggagcg cggcgacgat
     4621 attattgtgc tcgccaccca ttttttagag caggcgggta ataagctggg caaaaaagac
     4681 tgcaaactgc gcaaaagtga tttaaaactg ctgctggatt acccttggcc cggcaatatt
     4741 cgcgaattac aaaatgttat ggagcgcgcg gtaattaccg ccaaccaagg cagaattacc
     4801 ctggatttac caatggttga tccaaaaact gaagccccgc aaatagcaaa gccatccgag
     4861 caacggcaag tcatcgccga tgacgatatg cacgcccagg taaaagccaa tatgctcgcc
     4921 gccttagaag cctgcaattg gaaattattc ggcgaggatg gcgcagcggc cctcctgggc
     4981 ctaaaaccca ccacactcgc ctcgcgcatt aagcgcatgg ggctgaaaaa aacctaaagg
     5041 cagattggat aatttaagga accacagagc ttttaaattt cactatgagt atttagcaac
     5101 ttttattccc tttggaggca actcccgctc cactcaaaac aagcgggatt tggggttagt
     5161 gatgacaacc ccgcagtgat gactgcagat ttattgcaga cacgccgtaa atacatccat
     5221 gtaggctcgt caaaaagatc cctctttttg acggtctgca ataaacctgc agccaccacg
     5281 gctacaccgt gcatctattc cgtcgcaggg tttaactcgc caaatttttt caggtcaatg
     5341 ctgataggta cagattttct taccgcagaa acttgtaggg gcgccccttg tgggtgcccc
     5401 ttgccgagtc aatcccgagc caacgctaag gtgctcaatg ggcaaccaca agggttgccc
     5461 ctacgattgc gcatgattta ttgcattgtt agggatgcgg aagattaaaa ttgtggccgt
     5521 ttattactta aggaaaatat agacattgcg ccctgcccta cttcaataca gcagtaacta
     5581 cagagctatt ttttagcggc ttatcaaaac atcggtacac catttaaaaa taactaatcc
     5641 atcaaatccg gttagcattg ctggtgctga tatatactca gattctcaaa agaaataaac
     5701 caaggcaatc aagcattttc actttgaata cgcaaaacct gctcacttaa ccgctcagct
     5761 tgctcaagcg cttgctgggt gatactgggc aggtcgccat aaatgccggg aatatacacc
     5821 atgccgtggc tatacaccgg cggctggtag atcataccgg ccaagagtgc tgtctgttta
     5881 agcggcaata acagcgcctc cacttcaaaa tgattgtaac cgctagcatt gtaagactct
     5941 ttcggcccgc caatggttaa ggacacaata aaatgtttgc cttttaattt atcgccctta
     6001 gaaccatagg caaaattaaa cgccaacaca tcatccaacc atttttttaa cagcgcaggc
     6061 acggaatacc aataaaaagg cgcctgcaga ataataatgc ctgcatttat taaggcctgt
     6121 tgttcagcct caatattaat tttatagtcc gggtagaggc tatcaagcct gcgaatttcc
     6181 gccgccgggt ggcgcttggc aaactccgtc aaaatagttt ggttggcatt agaagatggt
     6241 aaatccgggt ggccggagat aattaatggc tttaacatgg cggcctcaca ttcaaaggtg
     6301 gagcggccat ggtaggggct tttatccgct gggattagcc tggagcaggc aaattcagta
     6361 tttggcccgg cttaataatg ccggtttgcc tgcccaaagc cgagacctaa tcccgcactg
     6421 atctcaatcg gtaattgatt ttttaaagcg cacaggtggc ccatgaaaag catagtgttc
     6481 gaacagttaa gggtttttca ggccattgca cgggccggtg gtatttccgc tgcagcgcgc
     6541 gcattgcagc tctccacccc cgctgtcagc caatccctca aacaattaga gcggcattta
     6601 ggccttaccc tatttcaccg ctccacccgc cacatggaac ttaccgccgc aggccattta
     6661 ttactgcaac agactctgcc cctcaccgat ggcctg
//
LOCUS       DAOQGS010000187         6654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5657_length_6654_cov_4.623428, whole genome shotgun sequence.
ACCESSION   DAOQGS010000187 DAOQGS010000000
VERSION     DAOQGS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_6654_cov_4.623428"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..2902
                     /locus_tag="PKZ52_08575"
     CDS             296..2902
                     /locus_tag="PKZ52_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNG59868.1"
                     /translation="MNIKKIAGLGTMSLLASLIAAPTMAANILQNGAFDGNKSSPWEF
                     GAWESSKATLSMSSYPGRACINVTSPGAESWMVQFRQNSMKYIDGRTYTLTADVWSSK
                     AITLKVDGSDETGGYVWQFGNTFAVSAPLAGSPQKITAVFSNKRTTDTGKLAFLLGNT
                     LVPADTVVCLDNIVLDDPQATGTDGPKVIPVAQVKVNQHAYLPTFAKKAVFVVPAGES
                     ASQARTWELRRGSSVITSGQTVNVGLDAASGDNVQTIDFSSISTEADDYTLVVKSGST
                     EYTSQPFAISADAYKKLKYEALSYFYHNRASTPILASVVGDTYARAAGHPDNAVQTAA
                     CASSPSSPDCATVDVSGGWYDAGDHGKYVVNGGISVWTLMNQFERAQNLGSNAAAFAN
                     GAMALPSSEQINGVPDILDEARWEVDWFLRMQASSGLVYQKVHTQGWTAIPTAPADDK
                     KPRVIQPVSTAATLNFAAVTAQCFRTFKAYDATYANKCLAASKKAYAAAKQNPSLPAV
                     DSDGSGTYGDKSMVDEFFWAATELYVSTGDSAYLNDMKTSTFYNTIFSGAAETLIPDS
                     SLGWPTTELLGVISLASAKGEYVRDASMVTKAQNFIVRRADAYVAESKNQGFGLPYKG
                     QNAGWGSNSEVVNNMIVVGLANDFTCAAKTDYVNTLQSGMSYLLGTNPLDQSYVTGYG
                     ERPTQAPHHRFWAKAASAAYPAPPAGVLAGGPNKAIEDSVAMVDLAGCTALKCYLDNI
                     NSYSTNEITINWNAPLAWVAAYLDEQSTANGRNATAKRCSGGSSSSSSSSSSSSSSSS
                     SSSSSSSGRPSSSSSSSSSSSSSSSSSSSSSSSSSSSGGVVLGGALGLVDMLAVLALA
                     AFGVFSRRRFKA"
     gene            complement(3319..4116)
                     /locus_tag="PKZ52_08580"
     CDS             complement(3319..4116)
                     /locus_tag="PKZ52_08580"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG59869.1"
                     /translation="MKHFRLKSLTTGNIFTLTQSTITIGRGAFNFVRLESDSLSSQHA
                     SLHIDNGRAVLEDLNSTNGTFINNMPVKAPTELMHGDVISLGNEKLMLIDPENQDDAT
                     VFSLNLGGATSLPASKVAALNTGTLSAEESQQVVSDVLKKAFASIAADTSEAQDISLL
                     ATLSKTGVESVFKLDTANPPAGTNWSIGRDEACALTINNPTVSTHHASLRFEDGQWFI
                     KDNGSTNGTKVNGKRVVESPCKSGDIISLGKLNCVFGVAQEVFTEVE"
     gene            complement(4365..5342)
                     /locus_tag="PKZ52_08585"
     CDS             complement(4365..5342)
                     /locus_tag="PKZ52_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HNG59870.1"
                     /translation="MEFGKVISVPLRDLWQGEATHFTPWLAKNLDILSEKIGLELDLE
                     EVEASAGDFSVDIVARDLSTNKLVVIENQYGLTDHKHLGQVVTYSSELGAAIVIWIAE
                     SIRPEHKRAIDFLNNNLKSSLQLYAIEAGIIKIDDSKPAFELNLVCMPNVSALTTSAA
                     LESTSETKEKYRTYFQSLIDELRINHHFTNAKAGQPQNWYTFSSENSKIYKYSTSFAH
                     GGRVRAEIYLDSGNKEKNEAIFDSIYQNKIAIENEFGSSLTWEKLDNKRASRIAIYRD
                     GDIDSDSETLTDIKNWTIANLLKLKKILPNHIETALNTIMQSNNHSQTS"
     gene            complement(5869..>6654)
                     /locus_tag="PKZ52_08590"
     CDS             complement(5869..>6654)
                     /locus_tag="PKZ52_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNG59871.1"
                     /translation="PEPTASQAFAGIYQLAECKRQCDAILAQVDLILTPAFPRAFTRA
                     EIDADPIATNSSLGTYTNFMNLLDYAALALPAGQHSNGLPFGITLFGPAFSDMALLSL
                     GRSFHLACGLPQGATPHRPPAHNLTSPATSSAYTDLLVCGAHLAGEPLNWQLQERSAS
                     LKAATHTAPGYRLYALPDGKRPALVRTPGSQTSIEVEIWRLPTHTLGSFLTGIAPPLG
                     LGQAQLADGSWVSAFICEPIALEGATDITEFGGWRGWKQSREN"
BASE COUNT         1466 a         1568 c         1749 g         1871 t
ORIGIN      
        1 tggcaaattc gcctagccag cgcttcgccg cctacatttt tcagaggttc cctaataccg
       61 tttgcttaat tttttgcatc ggcccaacaa aattaatcgc caatatttcg aatctaaggt
      121 tgaaaacgtg gctggtttaa agttttacaa ttgggcgatg tggtagattg gactaattga
      181 ttgcaatcgc caggcaaatg tgcgtgtttt tagtaatcaa tgtcgtgccg aatgatcggg
      241 cgcagggttg aacccgtccg gttgaaaaac ttatgtgttt tataggagtt tgattttgaa
      301 tattaaaaaa atagcaggct tgggcactat gtccttgctg gcaagcctga ttgccgcacc
      361 cacgatggct gcgaatatcc tgcaaaacgg ggcttttgac ggcaataaat catccccttg
      421 ggaatttggt gcttgggaat cgtccaaagc aaccctgagc atgtcgagct accccggccg
      481 cgcctgtatc aatgtgacca gccccggtgc cgaatcctgg atggtacagt ttcgccaaaa
      541 cagcatgaaa tatatcgatg gccgtaccta cacgctcacc gctgatgtgt ggtcctctaa
      601 agctatcacc ttaaaagtgg acggttccga cgagaccggt ggttatgtat ggcagtttgg
      661 caataccttt gcggtttctg cgccattggc gggttctcca caaaaaatca ctgccgtatt
      721 ttccaataaa cgcaccactg atactggcaa actagcattc ttgcttggca acacccttgt
      781 gcctgcggac actgtagtct gcttggataa catcgttctg gacgatcctc aagcaacagg
      841 tactgatggt ccgaaggtta tccccgtagc ccaagtgaaa gttaaccagc acgcttattt
      901 gcctactttc gctaaaaaag ccgtttttgt agtgcctgct ggcgagagcg catcgcaagc
      961 ccgcacctgg gaattgcgcc gtggctcttc agtgattact tcaggccaga cggttaacgt
     1021 cggcctcgac gccgcctccg gcgacaatgt gcaaaccatt gatttttcat ccatttccac
     1081 tgaggctgat gattacaccc tcgtggtgaa aagcggttcg actgagtaca ccagccagcc
     1141 ttttgccatc agcgctgatg cctataagaa gttgaagtac gaagcgcttt catacttcta
     1201 tcacaaccgt gccagtacgc cgatcctcgc cagtgttgta ggtgatacct atgcccgtgc
     1261 cgccggtcac ccagataacg ctgtgcaaac cgctgcttgt gcttccagcc caagctcgcc
     1321 cgattgcgcg acggtggatg tgagtggcgg ttggtacgat gccggtgacc atggcaaata
     1381 cgtggtgaat ggcggtatca gcgtgtggac cttaatgaat cagtttgagc gcgcccagaa
     1441 cctcggcagc aatgcagcgg cattcgccaa tggcgccatg gcattacctt cctctgaaca
     1501 aattaacggc gttcccgata ttctcgatga agcccgttgg gaagtagact ggtttttgag
     1561 aatgcaagcc tcctcaggct tggtgtacca aaaagtacac acccagggtt ggaccgcaat
     1621 tcctaccgca ccggccgatg ataaaaaacc ccgtgtaatt cagcctgtct ccactgccgc
     1681 cacgctgaat ttcgccgctg taacggctca gtgcttccgc accttcaagg cctatgatgc
     1741 aacttacgct aacaagtgtt tggcagcatc caaaaaagcc tatgcggccg ccaaacaaaa
     1801 tccgagctta ccggctgtgg actctgacgg ctcaggtacc tatggcgata aaagcatggt
     1861 tgatgagttc ttctgggcag ccactgagct ttatgtctcc accggtgaca gcgcttacct
     1921 caatgacatg aaaaccagca ccttttacaa caccatcttc agtggcgctg ctgaaaccct
     1981 aattcccgat agctctttgg gttggccaac gactgaactc ctgggtgtca tctctttagc
     2041 ttctgctaaa ggtgagtatg tccgcgatgc ttccatggta actaaggcgc aaaactttat
     2101 tgttcgccgt gccgatgcct atgtggctga atcgaaaaac caaggttttg gcctgcccta
     2161 taaaggccaa aacgctggtt ggggttctaa ctctgaagtg gttaacaaca tgattgtggt
     2221 gggtcttgcc aatgacttta cctgtgcggc caagactgat tatgtgaaca cactgcaaag
     2281 cggtatgagt tacttgctgg gtaccaaccc attggatcag tcctacgtga ccggttatgg
     2341 cgagcgtcca actcaagcgc ctcaccaccg tttctgggct aaggctgcta gcgctgctta
     2401 tccagctcct ccagcgggtg tgcttgctgg tggccccaac aaggctattg aagattcagt
     2461 tgccatggtt gatttggccg gttgcaccgc gctgaagtgt tacctggata acatcaactc
     2521 atacagcacc aatgagatca ccatcaactg gaacgcgcct ttagcttggg ttgccgccta
     2581 cttagacgag cagagcaccg ccaatggccg caatgctact gctaagcgct gcagcggtgg
     2641 ttcttctagc agtagttctt caagcagctc atccagcagt tcaagctcat catcaagctc
     2701 tagctcgtca ggtcgtccat ccagctcttc tagcagctca agctctagca gcagctcaag
     2761 ttcgtccagc tctagctcat cttccagcag ctcatccagc ggtggtgttg tattgggtgg
     2821 tgcattaggc ttggtggata tgttggctgt attggcactg gccgcctttg gtgtgttctc
     2881 acgccgtcgc tttaaagctt aatcgttaag tttttaaagc tacaaaaaag cctacaccct
     2941 tgggtgtagg cttttttttg tcttgtgctt tatgtacgtt caagtgctca ggatattttt
     3001 aaccgtatga acggcacgcg cgtttttctt gagtgctatt agcccggcgg caaaatagat
     3061 cgtcctgatc tattttgcct tcgctgcact aaaacaggcg agaatgcgcg gattttcttg
     3121 ttttagcgca gctcgcggcg gtgtacacct ttgacgcggc atccatgccg ccattattaa
     3181 cccggcacta attaccgccg ggttaataac tcattactgt acccgcgttg gtattcactg
     3241 ccagatgtac gatgaaatca ggaacattgt tactggctgg tggtgttcgg tttaatttgg
     3301 gttgttattt aatttgcctt attcgacctc tgtaaaaact tcctgggcca cgccaaatac
     3361 acaatttaat ttacccaacg aaataatatc gccgctttta catgggctct ccaccacgcg
     3421 tttgccattc accttagtgc cattggtgga gccgttgtct ttgataaacc actggccatc
     3481 ctcaaacctt aggctcgcgt ggtgggttga gacggtgggg ttattaatgg tgagggcgca
     3541 ggcttcgtcg cggccgatgg accagttagt acctgcaggc gggttggcgg tgtcgagttt
     3601 aaaaacggac tccacaccgg ttttagaaag ggtggccaat aggctgatgt cttgagcctc
     3661 tgatgtgtct gctgcgatag aagcaaaggc ctttttaagt acatcgctca ccacctgttg
     3721 gctttcttca gcgctgagtg tgccggtatt gagtgcggcg actttagagg cgggcaggct
     3781 ggtggcgccg cctaggttta aggaaaacac cgtggcatcg tcctggtttt ccgggtcgat
     3841 cagcatgagt ttttcattgc cgaggcttat cacatcgccg tgcattaatt cggtgggcgc
     3901 tttgaccggc atattgttga taaatgtgcc gttggtggaa tttaagtctt ccaacaccgc
     3961 ccggccatta tcgatatgca ggctcgcgtg ttgggaagac agcgaatcag actctaaccg
     4021 cacaaaatta aacgcccctc ggccaatggt gatggtggat tgggtgagcg taaagatatt
     4081 gcccgtggtg aggcttttta aacggaagtg tttcatggcg gtgtcctctg gggggttgtt
     4141 ggggcggtgg cgggttttgc tcggcagttt aactgtagtt tgcccatgga ttggaaaagt
     4201 atttcctcgt ttttgtagcc ttctattatc gagactttgc aagaatgtct atttcaccga
     4261 ctatcaaatt atacttgggc ctttgcactc accttgcggt gagtcaatcc tcttccgttc
     4321 agattcgtgt tggctcaaaa aacgcaggta ttttgtgttc tgatttagct ggtttgtgaa
     4381 tggttattgc tttgcattat tgtattgagc gcggtttcga tatggtttgg tagaattttt
     4441 tttagtttta gtaagtttgc tatcgtccaa tttttaatat cggttaatgt ttctgagtcc
     4501 gaatctatgt cgccatcacg atagatggcg atcctggatg ctcttttgtt atctagtttc
     4561 tcccatgtca aagagcttcc aaattcattt tcaatcgcga ttttgttttg gtaaatggag
     4621 tcaaatatgg cttcattttt ttctttattg ccactatcca aataaatttc tgctctaact
     4681 ctgccgccat gagcaaagct ggttgagtat ttgtatatct ttgagttttc tgatgaaaat
     4741 gtataccaat tttgaggttg gcccgcttta gcattcgtaa agtggtggtt gattcgcagt
     4801 tcatcaatca atgactgaaa atacgtgcga tacttttcct ttgtttcgct tgtagattct
     4861 aatgctgcag atgtcgttag tgctgaaaca ttaggcatgc atactaggtt taactcaaac
     4921 gctggttttg agtcatcaat ttttatgatt cctgcttcaa ttgcataaag ttgtagggac
     4981 gactttaagt tgttgttcaa gaagtcgatc gcacgcttgt gttctggtct aatggactct
     5041 gcaatccata ttacaatagc tgcgcctaat tctgatgagt aggtgacaac ttgcccaagg
     5101 tgtttgtgat cagtaagacc gtattggttt tcaattacca ctagtttgtt ggtggataag
     5161 tctcttgcca ctatgtctac agaaaaatcg cccgcagaag cttcgacttc ttctaggtct
     5221 aattcaagcc ctattttttc cgacaggatg tctaagtttt ttgctagcca tggagtaaag
     5281 tgtgtggctt cgccttgcca tagatcccgc aaaggaacag agatgacttt tccaaattcc
     5341 ataaatacat tcctcatgta agtgttgttt tttatcgttt tcgctggcta tgggttttct
     5401 tgtttatttt tatttggctt tcgactatgg aaggtgtgtc agcaaatgaa tgggcggctt
     5461 aatttttgag cttattttcc cattgttcag aatctcgaaa ccgaccgatg tttatagatg
     5521 tttctagata atttcacaag tagataactt aaaggcaata gctctacatt gctgtgacag
     5581 ggcgatttac aaccgcgcaa aggcagtccc aaccgtgtct aaaacgcctc tgaaatcata
     5641 tccctcacgg agggatttca tagtaattgg gtcggagatc aggtcattgg aatcgcgatt
     5701 aactagtgga ttgccaaagg gcacacatta aatcctaaat tcggttgggg gctaggggag
     5761 cagatccgat ttttgggttg cgtgttctgt gggagatgcc ctttggctac ccctatggaa
     5821 tttaataatt gagatggctg ctgccaagcg catgttaagt taacccattt aattctcgcg
     5881 gctctgtttc cacccccgcc agccgccaaa ctcagtgatg tccgtggcgc cctcaagggc
     5941 gatgggttcg cagataaaag cgcttaccca gctgccgtcg gccagttgcg cctggccaag
     6001 gcccaaaggt ggggctatgc cggtgaggaa gctgcctagc gtgtgggtgg gcaggcgcca
     6061 gatttccact tcgatgctgg tttggctgcc gggtgtgcgt accagggccg ggcgtttgcc
     6121 gtcgggcagg gcgtagaggc gatagccggg ggcggtgtgg gtggcggctt tgaggctggc
     6181 gctgcgctcc tgcagttgcc agttgagagg ctccccggct aggtgggcgc cgcagactaa
     6241 taagtctgta taggcgctgg aggtggcggg gctggtgagg ttgtgggcgg gtggcctatg
     6301 gggggtggcg ccctggggca ggccgcaggc gagatgaaag ctgcggccga ggcttagcag
     6361 ggccatatcg ctaaaggccg ggccgaatag ggtgatgcca aagggcaggc cgttactgtg
     6421 ttggcctgcc ggtaaggcta gggcagcgta gtccagcagg ttcataaagt tggtgtaggt
     6481 gccgaggctg ctgttggtgg cgatggggtc ggcgtcgatt tcggcgcggg taaaggcgcg
     6541 cggaaaggcg ggggtgagga tcaggtccac ctgggcgagt atggcatcgc actggcgctt
     6601 gcactcggcc agttggtaga tgcccgcaaa cgcctggctt gcggtgggtt cggg
//
LOCUS       DAOQGS010000188         6651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5661_length_6651_cov_4.006216, whole genome shotgun sequence.
ACCESSION   DAOQGS010000188 DAOQGS010000000
VERSION     DAOQGS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_6651_cov_4.006216"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1121)
                     /gene="rimK"
                     /locus_tag="PKZ52_08595"
     CDS             complement(153..1121)
                     /gene="rimK"
                     /locus_tag="PKZ52_08595"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HNG59872.1"
                     /translation="MLEACYARYKCDDVPGLKIGLLASNPELFSNKRIMLAGEERGHQ
                     MFFLSLKQCYMKLDADTPEIHYRGGNILETLDAVIPRIRPSMTFYGCALTRQFESMGT
                     YALNSSSAIAQSRDKLFSLQLLLKNGIDIPITGFANSPSDTQDLINMVGGAPLIVKLL
                     EGAQGRGVVLAETRKAAESVINAFKAVQANLLVQEFIKEANGRDLRCFVIDGKVVAAI
                     ERTAAPGDFRANIHQGGTATVAKITADEKKLAIKAARTMDLSVAGVDIIRSSKGPLLL
                     EVNSSPGLEGIETATGRDIAGLMIQSIEKRLNWRRKLATELDAIKK"
     gene            complement(1149..1571)
                     /locus_tag="PKZ52_08600"
                     /pseudo
     CDS             complement(1149..1571)
                     /locus_tag="PKZ52_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
     gene            2286..3293
                     /locus_tag="PKZ52_08605"
     CDS             2286..3293
                     /locus_tag="PKZ52_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNG59873.1"
                     /translation="MPFSQFIAHRIERQNPQALVNLKLRHQTLELNGKSEDLAREVKK
                     AYIKKTGKAYGRFSSDCAAHPLSQWLHEYRAGKLSFTSFTHSALTHFKLEIEKCESLV
                     YSHILIAEEVLEGAEFLYFILLDHQNAWYFDAEVELGDSLFLDPSQFTAGARINLREW
                     EGQENNNYISIVKFRNDKDFSEAFINFIGFTDKVDQKAQTAELIEVVEQYAGQLDEQA
                     ASQTRHTLVNYCLEQAKQDKPVIIEELSQQINHEKSAEIAQFIQSNAQSLHKEIIPDT
                     GALRSLVRLSGRNEVMSMSFASDCIGSTVVYDTDSDSLIIKSIPPALKTRLLKLLRQK
                     N"
     gene            complement(3375..6209)
                     /locus_tag="PKZ52_08610"
     CDS             complement(3375..6209)
                     /locus_tag="PKZ52_08610"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNG59874.1"
                     /translation="MAVGLLTLCARVWALGEASYLSERKVPGAMPLFGGGQAAEIYVD
                     PQDFPGAQRAARDFSADLQKIGGPALNLVGDGGELNEYAVIIGTLGHSRLVDSLVQRG
                     VINTRELVAWDGYLVELVEKPLPQVKQALVVVGGNKRGTSYGVYDISQQMGISPWYYW
                     ADVTPLKKKALFVLPKTRKVDAPKVKYRGIFLNDEAPALTNWVTENHGNYNHEFYAKV
                     FELLLRLKANYLWPAMWNNAFNDDDPKNQALADEYGIVMGTSHHEPMMRADKEWNRYG
                     KGKWEFSTNPENLAEFWRQGAMRARPYENLFTMGMRGQEDQPMSEGENIQLLQNIVER
                     QRAILADVFAPQPVEKIPQVWCLYKEVQAYYEKGMRVPDDITLLWSDDNWGNVRRLPT
                     PAERHRSGGAGVYYHFDYVGAPRSYRWINTVPLAKIWEQMNLAYQFGAKQIWITNVGD
                     LKPMEFPTEYFLNLAWNPEAWDKNRIPEYTELWAEREFGKPLAKEIAALMEGYTRHNG
                     RRKPELMSPDTYSLFNYSEAERILSQLQQLTDKAEALSKKIPTERQSAYFQLVLHPVL
                     ATANITRLNIALGKNRLYAQQGRATANQYAQLAKEYFAFDKSLKERFDTFNNGKWRHF
                     MDQTHIGYTNWNHPEGDQLPALAEYLAGNYAEMGVAVEGISAAWPTPGNYALNFDSKG
                     KTQRSFTLFNRGTQPFDFSVTASPWIRLSQNDGKVDAELHINSSIDWAKLPEGTSIGQ
                     IVIKGTGWQAAKISVKAVKASNEINKRARGFIEADGYVAIEAAHFKKSTVKEGIAWQE
                     IPQHGRTLSSIMPLPVADKSFTDIKQAPYVEYPLTLFTTGAITVETVLAPGLPFVPGR
                     GLRYAISIGDEEPKIIDFLAGYKDADANWEAAVENGARIGRSEHYISKAGPQILRLYV
                     VDPGVTVQRLQINTGGLLPSYLGPPESVYR"
BASE COUNT         1774 a         1687 c         1465 g         1725 t
ORIGIN      
        1 cgccaaattt tggcaaaaaa gcgcccatat ccagcgtctt aggcaccctc gctacgagtt
       61 caactgcggt ttttaggttt aaatctattg tttaatgccc aagcagagta tgactcggcc
      121 ttgccaggac actgaatcgc cattgtgtgc tgttattttt taatcgcatc taattctgtt
      181 gccagtttgc gccgccaatt taagcgtttc tcgatcgact gaatcattag gccggcaata
      241 tctcgcccgg tagcagtttc aataccttca agccccggcg acgaattcac ttcaagtaat
      301 aatgggccct tactggagcg aataatatcc accccggcca cactcaaatc catggtgcgg
      361 gcggccttga tggccaattt tttttcatcg gcggtaattt ttgctactgt cgctgtaccg
      421 ccttggtgga tattagccct aaaatccccc ggcgctgctg tgcgctcaat agctgcaact
      481 actttgccgt caataacaaa acagcgcaga tcacgaccat tggcttcttt gataaattcc
      541 tgcaccaaca aattcgcctg cacggcttta aaagcattaa tcacactttc ggctgctttg
      601 cgcgtctccg ccaatacaac accacggcct tgtgcgccct ccaataattt aacaatcaac
      661 ggagccccgc ccaccatatt aattaaatcc tgggtatcgc tgggcgaatt agcaaagccg
      721 gtaataggaa tatcaatgcc gtttttcagc agcaactgca gggaaaataa tttatcccgc
      781 gattgcgcta tagccgatga gctatttaaa gcataagtgc ccatgctttc aaattgccgt
      841 gtgagcgcac agccataaaa ggtcatacta gggcgaatgc ggggtataac cgcgtcgagg
      901 gtttctaaaa tattgccgcc acggtaatga atttccggtg tatccgcatc cagcttcatg
      961 tagcattgtt ttaaacttaa aaaaaacatt tggtggccgc gctcttcccc ggctagcata
     1021 atgcgtttat tactgaataa ttccgggttg ctcgccaata aaccgatttt aagcccaggc
     1081 acatcatcac acttataacg ggcataacag gcctccagca tttctttgct gcggcttccc
     1141 tgtaaaaatc tctgctccgg gtctactaac aggcggccca ccatggcctg ccgtcccaac
     1201 agcattcggt accccatgga gtcgcgattg gtgagggtta attcgatatc aaaaatatcc
     1261 tcacccagct gcaatggtac tcgcaccacc aaacgggttt ccgtttcgcc gctggaacta
     1321 cgcactttac gtttatcaat caccgcagct tcacagcgca caacaatttg ccgagattgt
     1381 tgcaatggat gcacctcaaa actcacccag ggctggccgt gtttgcgaaa tggcgcaata
     1441 ttaatcgcgt gaatactcga tgttttagcg cctgagtcca cccgagcttt aatggcgggc
     1501 agcccaaggc ttggcagtgc gcaccattct tcagagccta taagggtttt attttgcgac
     1561 tctaaagcca tcggcgtacc tttttacaat agtgtgtata gtccgcgcca aattgacgct
     1621 caagataata ttcctcgaca gctatttgcc aagtgttaat ccaaataaaa aacaccgcgc
     1681 agggaataaa tgccaaaccg tcggcacacc aaacggccaa cccaatcata aaaattaaca
     1741 tacctaaata aatgggatta cggctccaac caaatacacc cgaggccagc agagtggtcg
     1801 tgcgggtaaa gttcatcgga tgcacagacg tttgatgccg cctaaactga ataaaagcca
     1861 ataaaattat tatcgcccca gataagcaaa ataaaagcgc taaaaatacg gggatattag
     1921 gcagcggcac ccaatgcagc cagctcaatc caaaggcgat gagggacaaa agtattgcca
     1981 gcgctaatgg aggtataaat ggtgcgcgag tggacatagg ttatcgaata tgggctacaa
     2041 aggcctatta tgcagcaaaa atatccctgc tacgacccat agacctgcta agaatgcagc
     2101 tgggccgcct ccaccaccta ctccccattc atgtcaataa tattaacccg gtggcttttt
     2161 atgccgggtt aatagtaatg cgtaaaagtg agcgccaacg caagcacacc ccagtgaata
     2221 tggcttgcac cctagcagct taggcactca gtaggtaaac tggcggtttt tagctggatt
     2281 cctctatgcc ctttagtcaa tttattgctc accgaatcga gcgtcagaac ccgcaggcac
     2341 ttgtcaacct taaattgcgt catcaaaccc tagaattaaa tggcaaaagt gaagatctgg
     2401 cgcgagaagt taaaaaagcc tatatcaaaa aaaccggcaa agcctatggg cgattctcat
     2461 ccgattgcgc ggctcaccct ttaagccaat ggctgcacga ataccgcgcg ggcaaactca
     2521 gttttaccag ttttactcat agtgcattaa cacactttaa attggaaatt gagaaatgtg
     2581 agtccttggt atacagccac atattgattg cagaagaagt tttagaggga gcagagtttc
     2641 tgtattttat cctgctcgac catcaaaatg cctggtattt tgacgccgaa gtagagctag
     2701 gtgatagctt attcctcgac cctagccagt ttaccgcggg ggcacgcatt aatttgcgcg
     2761 aatgggaggg gcaagaaaac aacaattaca tctccattgt taaatttcgc aacgataaag
     2821 atttttctga ggcctttatt aattttatag gttttacaga taaagtcgat caaaaagcgc
     2881 agacagctga actcatcgaa gtggtagaac aatatgctgg gcaattggat gaacaagccg
     2941 ccagccaaac gcgccatacg ctggtgaatt attgtttaga gcaggccaaa caagataagc
     3001 cggtaataat tgaggagctt agccagcaga ttaatcacga aaaatccgcc gagatcgccc
     3061 aatttattca aagcaacgct caatcgctgc acaaagaaat tattcccgat actggcgctc
     3121 tgcgcagcct agtgcgcttg agtggccgca atgaggttat gagcatgagc tttgcgtccg
     3181 attgtatcgg ctccacagtg gtatacgata cggattcgga tagtttaatt ataaaatcta
     3241 ttccaccggc acttaaaaca cgcttactta aactattgcg gcaaaagaac taataaatct
     3301 ttgattgagc ccgaaggcgg gctatcgtgc taatgtgcct gcgcatcggc atctaaccga
     3361 tgcgcacgcg cgggctaacg gtacacactt tccggcggcc ctaggtaact aggcaacaat
     3421 ccaccggtat taatctgcaa ccgctgcact gtcacaccag ggtcaacaac ataaaggcgc
     3481 agtatttgtg ggccagcctt actgatgtaa tgttcactgc ggcctattcg cgcgccgttt
     3541 tcaaccgcgg cctcccagtt ggcatcagcg tctttatagc cggctaaaaa gtcgataatt
     3601 ttaggttctt catcgccaat actaatggcg taacgcaagc cccgcccggg cacaaagggt
     3661 aagcctggcg ctaacacggt ttcgacagta attgctccgg tggtaaataa tgtcaacggg
     3721 tattcaacat aaggcgcttg ttttatatcg gtaaaacttt tatccgcgac gggcagcggc
     3781 attatggagc ttagggtacg cccatgctgc ggaatttctt gccaggcaat cccttctttg
     3841 acggtactct ttttaaaatg agcggcctca attgccacat aaccatccgc ctcgataaaa
     3901 ccacgggcac gtttattaat ttcattagat gccttaacgg cttttacgga aatttttgca
     3961 gcttgccagc cagtgccttt aataacaatc tgcccgatag aagtcccctc aggcaatttt
     4021 gcccaatcaa tactgctatt aatatgcagt tctgcatcta ctttgccgtc attttggctt
     4081 aaacgtatcc agggcgacgc cgttacgctg aaatcaaagg gttgggtacc gcgattaaat
     4141 agggtaaaac tccgctgggt tttccccttg ctgtcaaagt ttaatgcata gttgccaggt
     4201 gttggccaag cggcgctgat gccctccact gcgacgccca tttccgcata attaccggct
     4261 agatactccg ctaaagcagg taattggtcg ccttcaggat gattccaatt ggtataacct
     4321 atatgggttt gatccataaa atggcgccac ttgccattat taaacgtatc aaaacgctcc
     4381 tttaaacttt tatcaaacgc gaagtattct ttggcaagtt gcgcgtattg atttgcagtt
     4441 gcgcgcccct gttgggcata gaggcgattt ttgccgaggg caatattcaa ccgcgttata
     4501 ttggcggtgg ccaaaaccgg gtgcaacacc agttggaaat aggccgactg acgctcggtg
     4561 ggaatttttt tactgagcgc ttcagcctta tcggttagtt gttgcaattg gcttaaaatt
     4621 cgctccgctt ctgagtaatt gaataaacta taggtgtcgg gcgacatcag ctccggttta
     4681 cggcggccat tgtggcgggt atacccttcc atcagggcgg caatttcttt agccaaaggt
     4741 ttgccaaatt cccgctcagc ccacagctct gtgtattcag gaatacggtt tttatcccaa
     4801 gcctcaggat tccacgccag atttaaaaaa tattcggtgg gaaattccat gggttttaaa
     4861 tcccccacat tggttatcca aatttgcttg gcgccgaact gataagccaa gttcatttgt
     4921 tcccaaattt tcgccaatgg aactgtatta atccaacgat aggagcgcgg cgcgcccacg
     4981 taatcaaagt ggtagtacac accggcacca ccactgcggt ggcgctcagc aggcgtaggc
     5041 aagcggcgca cattacccca gttgtcatcg ctccacagca gcgtaatatc atcgggtacg
     5101 cgcatgccct tttcataata ggcttgcact tctttgtaca aacaccagac ctgggggatt
     5161 ttttctaccg gttgaggcgc aaaaacatcg gctaatattg ccctttgacg ttcgactata
     5221 ttttgcaata gttgaatatt ttcaccttca ctcattggct ggtcctcctg accgcgcata
     5281 cccatggtaa ataaattttc gtatgggcgg gcgcgcatag cgccctggcg ccaaaactca
     5341 gccaaatttt cagggttggt ggaaaactcc catttgcctt tgccgtagcg attccattct
     5401 ttatccgcac gcatcatggg ttcgtgatgg gaggtgccca tgacaatgcc gtactcgtcg
     5461 gcaagcgcct gatttttggg gtcatcatca ttaaaagcat tattccacat ggcaggccat
     5521 aaataattcg cctttaggcg caataataat tcaaatacct tggcgtagaa ctcatggtta
     5581 taattgccgt ggttttctgt tacccaattg gttaaggcgg gggcctcatc gtttaaaaaa
     5641 atccctcggt atttaacttt gggcgcatct actttacgag ttttaggcaa tacaaaaagc
     5701 gcttttttct tcagcggcgt cacatcggcc cagtaatacc agggcgatat gcccatttgt
     5761 tgggaaatat cataaacgcc atagctggtg ccacgtttat tgccgccgac aaccaccagc
     5821 gcttgtttta cctgtggcaa tggtttttca accagctcaa ctaaatagcc atcccaagct
     5881 accagctcgc gggtattaat caccccccgc tgcaccaaac tatccaccaa acggctatgg
     5941 cccaaggtgc caataatcac cgcatattcg ttcaactcgc ccccatcgcc caccaggtta
     6001 agagccgggc caccaatttt ttgcagatcc gcactaaaat cgcgagcggc cctttgcgcg
     6061 ccaggaaaat cctgcggatc gacataaatc tccgccgcct gaccaccacc aaacaacggc
     6121 attgcacctg gcactttgcg ctcgcttagg tagctcgctt cacctaaggc ccatacgcgg
     6181 gcgcagagcg ttagcaaacc aacagccaca agacacctat gccacaggcg agaggcgttt
     6241 ttttcacatt gggttttatt cacaggattg tgctccggtt tatttttatc tgttggattc
     6301 tctgattaag gctgcagatg cgaatagtat gcaaccttgc aggcagaaaa cccacaacgt
     6361 gtgtgctcgc aaatattcac gaattcagac ttaatctgag tattctataa tttaattccc
     6421 accgcccgca gaaatactga cgatggcaag gcgattttaa aagcagagtt actacagctg
     6481 gttttttgtc atttttatgc tgcaatgccc atataaaggc caaaatccag cggctgcaac
     6541 gtttgcttga gaagcgaatt tggtttagga ctaaaccctg ccacacatcc agagtttgat
     6601 gctaaaagac tatatatatc aaggtcgtat ctgggtcagt gtgactataa t
//
LOCUS       DAOQGS010000189         6650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5662_length_6650_cov_4.453677, whole genome shotgun sequence.
ACCESSION   DAOQGS010000189 DAOQGS010000000
VERSION     DAOQGS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5662_length_6650_cov_4.453677"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKZ52_08615"
     CDS             <1..263
                     /locus_tag="PKZ52_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59875.1"
                     /translation="LQQGVSYQSFWTLTANPHILEQPIIMLLCRRLRKTPAQIFFRYL
                     VARGITPLTGTSDREHMQEDLAISEFELGADDLMAIDELLVP"
     gene            complement(495..941)
                     /locus_tag="PKZ52_08620"
     CDS             complement(495..941)
                     /locus_tag="PKZ52_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59876.1"
                     /translation="MTQQTSSPSRGDEYPLRYIIIHIHPVSARLRFLISQKNHLCLPL
                     PLPKLADLICITRKEHKIMAHPSSYIHACCSQWQIPEKDLAMVPEFRLWIDTPQGEIP
                     IYCLHVDAHQPFEAPAGTTWIELPNCFELSTLERHIMQEIYKWLLE"
     gene            complement(938..1468)
                     /locus_tag="PKZ52_08625"
     CDS             complement(938..1468)
                     /locus_tag="PKZ52_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG59877.1"
                     /translation="MKFIGVEHTQHASALADLINTAYRGTSGAKRWTSEHGLIEGARI
                     SIGGIRDLILDRNVRVFAAFDEDDMPRCCIAITFDGSVAELGAFAVHPDEQGSGLGTL
                     LLSFAEQYAAKRAQTLRVCVVNKSDKLLNFYVRRGYSFCEHNYPYPTHKLVGTPLVAG
                     LALVVMEKLAPQFNYQ"
     gene            complement(1718..2086)
                     /locus_tag="PKZ52_08630"
     CDS             complement(1718..2086)
                     /locus_tag="PKZ52_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNG59878.1"
                     /translation="MATVYFSSPIMHKNTKLEAVAGKRTTLLGFAKEHTIPIPHECED
                     GRCGSCLIKVTHLDGERVKGMALTDRERVVLKSLGKLPAREEERANVNDIPPTYRLAC
                     QTIITDEDLLVEFTGEPGGA"
     gene            2384..2788
                     /locus_tag="PKZ52_08635"
     CDS             2384..2788
                     /locus_tag="PKZ52_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNG59879.1"
                     /translation="MQAPVSKSSAEGWPALKRYYLAASLQLPERGQLVLPCQYRGKPA
                     SMVVVRLNGLIYGFLNRCVHMPFRLDCEHASVIDPSANRIKCSMHGLVFDPLTGACQS
                     PTMCTGEKLMAIGLCEESQIIWLEDKDVTLQN"
     gene            3402..>6650
                     /locus_tag="PKZ52_08640"
     CDS             3402..>6650
                     /locus_tag="PKZ52_08640"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HNG59880.1"
                     /translation="MSVQPRAVSKLLVANRSEIAIRAMRAAAEMGITTVAIYSQEDRF
                     ALHRFKSDESYLVGQGKKPIAAYLDIEDIIRVAVDAGVDAIHPGYGFLSENPDFADAC
                     ARAGIIFIGPSPDVMRTLGNKVAARKAAEAAGVPVMPASGPLPADIEQCKHIANTIGY
                     PFMLKASWGGGGRGMRVLENEADLLALVDVARREAQAAFGNDEVYLEKLVRNARHVEV
                     QILGDLAGNVVHLFERDCSMQRRNQKVVERAPAPYLDEATRQHLCDCALRLARQVGYS
                     CAGTVEFLLDADTNQAYFIEVNPRIQVEHTVTEEVTGVDLIKAQIRLAQGHLIGTEAS
                     GVPAQENIQLSGFAVQCRVTTEDPENGFTPDYGRIEAYRSAAGFGIRLDAGTAYPGAV
                     ITPYYDSLLVKVTAWSREYTDSIAKMDRALREFRVRGVTTNLNFLVNLINHPSFTAGT
                     YSTRFIDQTPELYKFAKKRDRASRLLRFVAEVSVNGHPDALGRPAPASRAPAPLPSIA
                     PGAILPGSKDRLNELGPAALGQWMRSQQQVLITDTTMRDAHQSLLATRMRTQDMLNIA
                     PYYASLLPELFSLECWGGATFDVSMRFLQEDPFGRLHRLRKAVPNILLQMLLRGANGV
                     GYTSYPDNVVKFFVRQAAECGVDVFRVFDSLNWVENMRVAMDAVIDSGKFCQATLCYT
                     GNMLATGSNKYNLNYYVQMAKELEKAGAHGLAIKDMAGVARPVAIKQLVTALRQEVGL
                     PIAFHTHDTSGIGAASILAAIEAGVDSVDAAMDAFSGLTSQPNLGSINQALRDTDRAT
                     GLAVEPLQAISDYWEGVRKYYTGYESEIRSGTADVYRHAMPGGQYTNLREQARSLGIE
                     HRWDEVAEAYAQVNQLFGDIVKVTPTSKVVGDMALMMVTQGLTPEDVQNPTREINFPE
                     SVVQLFRGDLGQPPGGFPLELSKKVLKGETPRSLRPGAELPDADLAAIRAEAAAKVGY
                     ELNDWALATYLMYPKVFTDFAKQLELYGDVSILSTPVFFYGMDLREEFSVSIEKGKSL
                     MVRLLAISEPDLEGFRKVFFELNGQQRIVKVLQKGLESQAQSHPKAEEGNPHH"
BASE COUNT         1514 a         1773 c         1786 g         1577 t
ORIGIN      
        1 gtttgcaaca gggcgtgagt tatcaaagtt tttggacctt gacagccaac ccgcacattc
       61 ttgagcagcc tataatcatg ttgctgtgtc gtcgcctgcg caaaactccc gcacaaattt
      121 tctttcgtta tttggttgcg cggggtatca caccgcttac aggcactagc gatcgtgagc
      181 atatgcagga ggatttggct attagtgaat ttgaactggg ggccgacgat ttaatggcaa
      241 ttgatgagtt gttagtgcct tagcgattct ttcgccgggc tattaaccag gcggcaaaat
      301 aaattattct gatctatttt gcctgcactg cactaaacgt aggtaaatgc gcggattttt
      361 tgttttagtg cagctcgcgg ctgcttgttg ttttagcgca gctcgcggcc gcttgcacct
      421 ttggcgcggc atccctgccg ccattattaa cccggcacta aatgctgccg ggttaataaa
      481 tagttacctt ttgttcactc caacagccat ttataaatct cctgcataat gtgccgctcc
      541 agggtgctta actcaaaaca attgggtaat tcaatccagg tggtgcccgc tggtgcttca
      601 aatggctggt gtgcgtccac atgtaaacaa taaataggga tttctccttg cggtgtatcg
      661 atccagagcc taaattcagg caccatggct aaatcttttt ccggtatctg ccattggctg
      721 cagcaggcgt gaatataact gctggggtgt gccatgattt tgtgctcttt gcgggtgatg
      781 cagattaaat ccgcaagttt aggcagcggc aatggtaaac acaggtgatt tttttggctg
      841 attaaaaagc gcaggcgagc acttaccggg tgaatgtgaa taatgatgta gcgcagcgga
      901 tattcatccc cacgcgatgg gcttgaagtt tgttgtgtca ttggtaatta aattgaggtg
      961 ctagtttttc cataacgaca agtgccaacc ccgccactaa aggcgtgccc accagtttat
     1021 gggtggggta ggggtagttg tgttcacaga aggaataacc cctccgcaca taaaaattca
     1081 gcagcttgtc gctcttattc accacacaca cccgcagagt ttgggcgcgt ttggcggcgt
     1141 actgttcggc aaaacttaat aataaggtgc caaggccgct accctgttca tccgggtgaa
     1201 cggcaaaggc acccagttcc gcaacgctgc cgtcaaaagt aatagcaatg cagcagcggg
     1261 gcatgtcgtc ttcatcaaag gcggcaaaca cgcgcacatt gcgatctaaa attaaatccc
     1321 gaataccgcc aatactaatg cgtgcgccct caattaagcc gtgttcgcta gtccagcgct
     1381 tggcgccgga ggtgccgcga taggccgtat taattaaatc cgccagggcg ctggcgtgtt
     1441 gagtgtgttc aacaccaata aatttcatag tgcatacgtg ggcaagaaaa aagtaacgtc
     1501 aaattaaact ccgtaattaa caaccagacg cactgaggtg ttattaactc ggcggcattt
     1561 attgctgggt taataatggc ggcaaggatg ccgcactcgg cggtgccagc cgccgcgagc
     1621 tgcgttaaaa caagaaaatc cgcgcggttt cgtacgtttg gtgcggcgaa ggaaaaatag
     1681 atcagcgcga tctatttagt cgccgggtta atagtaatta tgcgccgccg ggctcgcccg
     1741 taaattccac caataagtct tcatcggtaa tgatggtttg gcaggctagg cgataggtgg
     1801 ggggaatatc atttacattg gcgcgctctt cttcccgtgc gggcaatttt cctaaggatt
     1861 taagcaccac gcgctctcgg tcggtcagtg ccataccttt tacgcgttcg ccgtctaaat
     1921 gggtaacttt aatcaaacac gagccgcagc ggccgtcttc gcattcgtga gggataggaa
     1981 tagtgtgctc tttggcaaaa cccagcagcg ttgtgcgctt accggcaact gcctctaact
     2041 ttgtgttttt gtgcatgatg ggcgagctga aataaacagt ggccatggga ggctccaata
     2101 aaaaataatg agtgaaataa gcttgtaggg cgcagccagg tggcaagttc tgttccaaat
     2161 accggcagcg gttttgccgt tgtcatgtca gttgacttgg atcaaccaag tggattggcg
     2221 caggcgtgac ggaaatttat gggttgattt tttacgtggc aactagatgg ctcaattctt
     2281 ggcaaaacta tggcgttaat tttgaataga ggtaaatgca agggttgtgg atggcatttt
     2341 tctatacgct cattttttac ttaggctttt gtggggcctg gctatgcaag cgccggtatc
     2401 aaaatcttca gctgaaggct ggcccgcgct taagcgatat tacttggccg caagcctgca
     2461 gttgcctgag cgcggccagt tggtgctgcc ctgtcagtac cgcggtaagc ctgcctccat
     2521 ggtagttgtg cgattaaatg ggctgattta tggtttttta aatcgctgcg tgcatatgcc
     2581 gtttcgctta gattgtgaac acgcaagcgt aattgaccct tctgccaacc gaattaaatg
     2641 ctccatgcat ggcctggttt tcgacccatt aacaggcgca tgccaaagcc ccactatgtg
     2701 cacaggtgag aaattaatgg ccatagggct atgtgaagaa agccagatta tctggctgga
     2761 ggataaagat gtaacactgc aaaattaacc cgcgcagaat tgctggctct tttttgttgt
     2821 aaagttgacg tttattctgt caacttcagt acattgttgt gtctgcgaca gaggtatttg
     2881 cctgagtaaa tgcgacagag gtatttgcct gagcaaaagt atttaactgt gaattcagca
     2941 gcataaaagt acacgcgggc gcaatcagat tttgcttaac gcacccattg tgtttttgcc
     3001 gcccgaagtc atttcttcaa aacaccgtgt ttttctccct gtggttaccc atcgcgtttc
     3061 gcaaccgtaa gttgtaaata tttcatactc aaaaatctcc ttccccaatt tttcctgtgg
     3121 gccatatttt atggcccatg aataacccac aaggcccggc agtggcgaga gtttaggcaa
     3181 ataaatacag aaggcggcta aacatttaat agttggacag gtaacaccga gctgcttggg
     3241 gatgctcttc agctgcctat tgtgcgctgc agtaggctgc ctctggccct gatcttgctg
     3301 ccgaaagcct tctgctatct gggttaaata ctatttgacc aacctgggcg tgcactgctt
     3361 atactgcact gcaaaacacc aatatgttta tgaggccgcc tatgtctgtt caaccccgtg
     3421 ccgtgtccaa gttattggtt gccaaccgca gtgaaatcgc catccgcgcc atgcgtgcag
     3481 ccgccgagat gggcatcacc acggtggcca tttactccca ggaagatcgc ttcgccctcc
     3541 atcgctttaa atccgacgaa agttacctgg tggggcaggg caaaaaaccc attgccgcct
     3601 acctggacat agaggacatc atccgggtgg cggtggatgc gggggttgat gccattcacc
     3661 ccggctacgg tttcctctcg gagaaccctg attttgctga tgcctgcgcc cgcgcaggca
     3721 ttatttttat aggccccagc ccagatgtga tgcgcaccct gggcaacaag gtggccgctc
     3781 gcaaggccgc tgaggccgcc ggtgtcccgg tgatgcccgc ctccgggcca ctgcctgccg
     3841 acattgaaca gtgcaaacac atcgctaaca ccatcggcta cccctttatg ctcaaagcca
     3901 gctggggcgg tggcggccgc ggtatgcggg tgctcgagaa cgaggcggac ctgttggcct
     3961 tagtggatgt ggcccgccgg gaagcccaag cggcctttgg caatgacgaa gtttatttag
     4021 aaaaactggt gcgcaatgcc cgccatgtgg aagtgcaaat cctcggggat ttggcgggca
     4081 atgtggtgca cctgttcgag cgggactgca gcatgcagcg gcgcaaccag aaagtagtgg
     4141 agcgcgcccc cgccccttat ttggatgagg ccacccgcca acacctgtgc gactgcgccc
     4201 tgcggctggc ccgccaggtg ggttattcct gcgccggtac cgttgagttt ttgttggatg
     4261 ccgacaccaa ccaggcttat tttatcgagg taaacccgcg tattcaggtg gagcacacgg
     4321 tcaccgaaga agtcaccggg gtggatttaa tcaaggcgca aattcgctta gcccaggggc
     4381 atttaattgg caccgaggcc agcggcgtgc ccgcccagga gaatattcag ctgtccggct
     4441 ttgccgtgca gtgccgggtg accactgagg atccggaaaa cggcttcacc cccgattatg
     4501 gccgcatcga ggcctatcgc agcgcggcgg gttttggcat tcgtttggat gcgggtaccg
     4561 cctacccagg ggcggtgatt accccctatt acgattccct gctggtgaag gtcacagcct
     4621 ggagccggga atacacagac tccattgcca aaatggatcg cgccctgcgg gagtttcgcg
     4681 tgcgcggggt gaccaccaat ttaaatttcc tggtgaattt aattaaccac ccgagtttta
     4741 ctgcgggcac ttactccacc cggtttatcg accaaacccc cgagctgtac aaattcgcca
     4801 aaaaacgcga ccgcgccagc cgcctgctgc gttttgtggc ggaggtgagt gtgaacggcc
     4861 acccggatgc cctcggccgc ccggcccccg ctagccgcgc cccggcgccg ctgccctcca
     4921 tcgccccggg ggcaattttg cccggcagta aagatcgcct caatgaactg gggcccgccg
     4981 ccctcggcca gtggatgcgc agccagcagc aggtgcttat caccgatacc accatgcgcg
     5041 atgcccacca gagccttttg gccacccgca tgcgcaccca agacatgctc aatatcgccc
     5101 cctattacgc cagcctgttg ccggagctat tcagcttgga atgttggggc ggcgccacct
     5161 ttgacgtttc tatgcggttt ttgcaggagg acccttttgg tcggctgcac cgcttgcgca
     5221 aggcggtgcc caacattctg ttgcaaatgt tgctgcgagg cgccaacggc gtgggttaca
     5281 ccagctaccc tgacaatgtg gtgaagtttt ttgtccgcca ggccgccgag tgcggggtgg
     5341 atgtgttccg cgtcttcgac agcctcaact gggtggagaa catgcgggtg gccatggacg
     5401 cggtgatcga ctccggcaag ttctgccagg ccaccctgtg ttataccggc aatatgctgg
     5461 ccaccggcag caataaatac aacctcaact actacgtgca aatggccaag gagctggaaa
     5521 aagccggcgc ccacggccta gccattaagg acatggcggg ggttgcccgc ccggtggcca
     5581 tcaaacaact ggtcaccgcc ctgcgccagg aagtgggcct gcccattgcc tttcacaccc
     5641 acgatacctc cggcattggc gcagcctcta tcctcgctgc cattgaggcc ggggtggata
     5701 gcgtggatgc cgccatggat gccttcagcg gcctcacctc ccaacccaac ctaggctcca
     5761 tcaaccaagc cctgcgggac accgaccggg caacgggatt ggcggtggaa cccttgcaag
     5821 ccatcagcga ttattgggag ggcgtgcgca agtattacac cggctatgaa agtgaaatcc
     5881 gctccggcac cgctgacgtg taccgccacg ccatgcccgg cggccaatac accaatctgc
     5941 gcgaacaggc cagaagcctc ggcatagagc accgctggga cgaagtggcg gaagcctatg
     6001 cccaggtcaa tcaattattc ggcgatatag taaaagtaac ccccacctcc aaagtggtgg
     6061 gggacatggc ccttatgatg gtcacccaag gccttacccc tgaggatgtg caaaacccca
     6121 cgcgggaaat taatttcccc gaatctgtgg tgcagttatt tcgcggcgat ttaggccagc
     6181 cccccggcgg cttcccgttg gaattaagta aaaaagtcct taaaggggag acgccgcgca
     6241 gcttacgccc cggcgccgaa ttacccgatg cggacctcgc cgccattcga gcagaagcag
     6301 cggctaaggt cggttatgaa ttaaatgact gggccttggc cacctattta atgtacccca
     6361 aggtatttac cgattttgcc aagcaactgg aactctacgg cgacgtgtcc attttatcca
     6421 cgccggtatt tttctacggc atggatttgc gcgaggaatt ttccgtcagc atcgaaaaag
     6481 gcaaatccct tatggtgcgg ctgctggcca ttagcgagcc ggacctagaa ggttttagaa
     6541 aagtattctt cgaattaaac ggccagcagc gcatagtaaa agtgctgcaa aaaggcctgg
     6601 aatcccaagc tcaatcccac cccaaagccg aagaaggcaa cccccaccac
//
LOCUS       DAOQGS010000190         6623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5701_length_6623_cov_4.566687, whole genome shotgun sequence.
ACCESSION   DAOQGS010000190 DAOQGS010000000
VERSION     DAOQGS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5701_length_6623_cov_4.566687"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(596..889)
                     /locus_tag="PKZ52_08645"
     CDS             complement(596..889)
                     /locus_tag="PKZ52_08645"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNG59881.1"
                     /translation="MRGFIVVDVNITDSEGFKEYAARIPALMEKHGGAYIVKAAVPKI
                     VREGAETPEYMVIIEFPSVAAADEFIAERSSSDLIDIFNRSTRGRILRVEGCF"
     gene            complement(1063..1431)
                     /locus_tag="PKZ52_08650"
     CDS             complement(1063..1431)
                     /locus_tag="PKZ52_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59882.1"
                     /translation="MYKIIALTLVLCTTSCATNHQAAISPPHPIDKEQFALELIALNV
                     QGQTRHIKYNTITGEAWWSADTSWTKIQDPEALPNSVYEIQAVATGDSWRAIRIDKVT
                     GKTWKNNSGKWVAFTHQVDQ"
     gene            complement(1707..2309)
                     /locus_tag="PKZ52_08655"
     CDS             complement(1707..2309)
                     /locus_tag="PKZ52_08655"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNG59883.1"
                     /translation="MRFILGGPPIDPFLASTDAIGWKPLTKTGFVKYSIAVAIATLIV
                     GGATLAALEYSGVFSYSVYAKSHADSVYFITLLSIVLIVVPHELVHLLLMPNFGLSNS
                     SMVGFWPKKIIFYAFHVGQMSKLRILAVVAGPLIVLTVMPIALSAALGVTSNVIGLAA
                     LSNALVTGIDIMTLCKLLLEVPKSGCIRQSGDNIFYLDHN"
     gene            complement(2435..2935)
                     /locus_tag="PKZ52_08660"
     CDS             complement(2435..2935)
                     /locus_tag="PKZ52_08660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNG59884.1"
                     /translation="MIVLREFKQEDVSRLQEHLNNRQVTQYLTSSIPQPYTLDDAKWW
                     INEGCKGGIVRAIDYNGQLVGSVGANRRQFEQARTGEIGYWIAQPYWGKGIATAALIE
                     LTAHILQASDILRLQAHVFAGNFASNRVLEKAGYSLEGVLRKAVCKQGVIMDAFLYAV
                     VRAENP"
     gene            complement(3314..3955)
                     /locus_tag="PKZ52_08665"
     CDS             complement(3314..3955)
                     /locus_tag="PKZ52_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNG59885.1"
                     /translation="MSAPVAGSQLPLHDEAHPAIECIQHFYSPEESAALYASLVSQQQ
                     WPDNHYSLAGRTFTLPRKQTWHADPGIHYSYSNNLLKTRPWTPLLTRLRHCVQTYLNC
                     EFNSVLVNWYRNGDDYVGWHADDEAELGPNPLIASLSLGATRTFSYKHKFHTISGSIQ
                     LTSGSLLIMQPSVQHQWLHAVLPEPEVTEGRINMTFRRVLSRAELMQAKGIKS"
     gene            complement(3952..4554)
                     /locus_tag="PKZ52_08670"
     CDS             complement(3952..4554)
                     /locus_tag="PKZ52_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59886.1"
                     /translation="MGLEFSVTKELASASATIIELVSVTKAIQTQLGKNDFVASFNGI
                     MANLSNCYSVIEDNFLPWLAVDSHAAFEAQFDNLFANYSANFLKEASKARPTVEQIYE
                     HYQELKTFKEFKTSFPMLKWAVSRLDDYIDKWIDNDTWFMMTLDRIFKMFNRFLLEIN
                     DFKRKDSDDAYVIFQLAISDFAALVHLIKDKRAELSQLTL"
     gene            complement(4659..5003)
                     /locus_tag="PKZ52_08675"
     CDS             complement(4659..5003)
                     /locus_tag="PKZ52_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HNG59887.1"
                     /translation="MSARFLLTSDETNRFFVNLVDANGELLLIGGTHPSRDEAEAAIK
                     EIRVGTLMSNQIGASKTPSGDTFFYIKNPGGQVIAKSQLFTSAMAFDNALHQVKDNTC
                     IAELDDLTAQNA"
     gene            complement(5579..>6623)
                     /gene="ald"
                     /locus_tag="PKZ52_08680"
     CDS             complement(5579..>6623)
                     /gene="ald"
                     /locus_tag="PKZ52_08680"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNG59888.1"
                     /translation="RVGMVPTSVAELTRQGHEVLVETQAGERIGFSDAEYLAAGAQIV
                     PDAKSAFAAGELIVKVKEPQLHECALIQPQQTLFTYLHLAADKPQALALMQSKARCIA
                     YETVTEPQGGLPLLRPMSEVAGRLAVHVGANYLHTAYGGRGVLLGGVPGVNPAKVIIL
                     GGGVAGGNAAQMAVGLHADVTLFDISAHKLIEINQLFNGRVKTAFSSAQAVAEAIKTA
                     DLIIGAVLIPGAAAPKLIRREQLKTLLPGAVLVDIAIDQGGCFESSHATTHQAPIFIE
                     EGIVHYCVANMPSAVARTSTLALNSATLPFIVKMANQGVQAALQKDPHLAAGLNIADG
                     VITHKAVAEALQL"
BASE COUNT         1720 a         1533 c         1597 g         1773 t
ORIGIN      
        1 ctgcccaggc acctgtacct aacaagagcg aaccggcaat tatacaatta ccgactgtgc
       61 ggcatattgt tacgggcaat tttgtatctc ggcatttgcc ataggtatcc atagtgaacg
      121 gtttcataaa aagtcctcat gaaattgaat tgtgtacgtt ttacgctcca aatgatttgg
      181 cacgcaactg aacaacaccg cgcagagaca gcagagttaa ttcatgtcag cgctatcgca
      241 accaggccta gctctttgat attgcataac atgccatatt taccgccgcc agccccataa
      301 aatcaggcgc acacgatgtt atccagcccg ctaaggctag gcgtgcaggc ttggttaaac
      361 ttcacgaatc tataacaaaa ggatggaaat ttatgattgc aggcttaagt gctgcgctct
      421 tgtgaagagg tggtaactaa caggaaaata tgaccattcc atttagatgg cagaatatca
      481 gagagcaaaa tttgccgggt gcaacgcaga atgtgtcccc tacccgctag caaaccagta
      541 aagtcgggca gccaggtaaa ggccggccca attaattttg aatttaatcg cgctgttaaa
      601 aacagccctc tactcgcaat attcgcccgc gggtagaacg attaaaaata tcgatgagat
      661 cagacgaaga ccgctcggca ataaactcat cggctgcggc gacgcttggg aattctataa
      721 taaccatgta ttcgggagtt tccgcgccct ctcgcacgat tttcggcact gccgccttaa
      781 ctatataggc gccgccatgt ttttccatca gagcgggtat gcgcgctgca tactctttaa
      841 aaccttccga atccgtaata tttacgtcta ctactataaa gcctcgcatt cacttatttc
      901 ccaatcaata ccttatttaa accggtggct atgaaaccaa tttaataaat agtcgcacaa
      961 acattgcagt caattcaaag gtagatagcc caagtgcaca aacgtatttt atcgggctat
     1021 aaactttaat ttccaaacca aaacacaggt taggtggtta atttattgat caacctgatg
     1081 ggtaaacgca acccatttgc cactgttatt tttccaggtt ttacctgtga ctttatcaat
     1141 ccgaatggca cgccaggagt caccggtcgc tacggcctgg atttcgtaaa cggaattagg
     1201 taacgcctct ggatcttgaa ttttagtcca gctggtatca gcagaccacc aggcctcccc
     1261 tgtaatcgtg ttgtatttga tatggcgtgt ttggccctgc acatttaaag caattaactc
     1321 aagtgcgaat tgctctttat cgatggggtg cggaggggat atagcagcct gatgattggt
     1381 ggcgcaactg gtggtacata acacgagggt taaggcaata attttgtaca tgttcattcc
     1441 tggattgaca attaagccat gattttagaa ttttttaact tgatgtttta ttttaaagca
     1501 atacacattt tttctaagca ttcaatttaa ggctgaagat aaaatgcgag atggtttact
     1561 ctgcggggac ctgttcaaat tgcacaccca atccaaccct accccaattt aggccctgca
     1621 agtaccatct tacgcctcat agatagcaag cattagacac ttgcccagac cactttttac
     1681 tactgattaa ggttaaaagc ctgccattaa ttatgatcca gataaaaaat gttatcgccg
     1741 ctttgccgaa tgcagcccga tttaggcacc tctagcaaca acttacagag ggtcattatg
     1801 tcgattccag ttaccagtgc atttgataat gcggccaaac cgatgacatt tgatgtgacg
     1861 cccaatgcag cagacaaggc aatcggcata acggtcaaca caattagtgg gccagctaca
     1921 accgccaaaa tccgcagctt tgacatttgc cccacatgga acgcgtaaaa aattattttt
     1981 tttggccaaa atccgaccat agagctgtta gatagcccga agttaggcat taacaaaagg
     2041 tgaaccagct catggggtac tacaatcaga acaatactaa gtaaagtgat aaaataaaca
     2101 gaatcagcgt gactctttgc atataccgaa taggaaaaaa cgcccgagta ttcaagggct
     2161 gctaaagtgg cgcccccaac aatcagggtt gcaattgcca cggcaataga atacttcaca
     2221 aaaccagttt tcgttaaggg tttccaccct attgcatcag ttgaagccag aaacggatca
     2281 atgggtggac cacccaatat aaatctcaaa tagatactcc ttaatgcctg ggcaattttt
     2341 aaacttcaga atcgatgagg ctagcgaaaa atcaaaacgc atccataaaa agcatggcga
     2401 aactaggcat cgcgacttca atagagttca ttttttatgg attttccgcg cgtacaacgg
     2461 cgtataaaaa tgcatccata ataacgcctt gcttgcaaac tgccttgcgc agaacacctt
     2521 ctaaactgta ccccgctttc tccaacacgc gattggaagc gaaattaccc gcaaaaacat
     2581 gagcttgcag ccttaggata tcgctggctt gcagtatatg agctgttaat tctatgagcg
     2641 cagccgttgc tatgccctta ccccaatagg gttgggcgat ccaatagccg atttcacccg
     2701 tccgcgcctg ctcaaactga cgcctatttg cccccaccga gccaaccaac tggccgttgt
     2761 agtcaatagc ccggactatg cctcctttgc agccttcgtt aatccaccat tttgcgtcat
     2821 ccagggtata gggctgcggg atacttgagg tgaggtattg cgtaacctgc cggttattta
     2881 gatgctcctg cagacggctt acatcttctt gtttaaattc acgtaacaca atcattgtga
     2941 ctccttagat cttattggat gtacccgttg ggaagctcaa agcaaattct gcatttgtta
     3001 caagcgcaga ttttgacaga tattatctta gggcgcccag ttaatcgagc atgttcatcg
     3061 gccctatggg cggattattt gtttatagct ttaaaaaata acctggccga gcacggtatg
     3121 ggcttgccct cacctttttg caggctaaag aggtaggttt gcaagctcct agttgaggga
     3181 agataggaac gtgctgggag ggaacttgct cggagatagg agcgagttgt ttgaattatc
     3241 gaatgcgcag acgaattgcc tgaagcggct tgcgctagat tagatgttca ccaacaacgt
     3301 ttatttaatt accctagctt ttaatgccct tagcttgcat taattctgcc cggcttaata
     3361 cgcggcggaa ggtcatatta atgcggcctt cagtaacctc gggctctgga aggacggcgt
     3421 gtagccattg gtgttgcacg cttggctgca taattaacag gctgccgctg gttaattgga
     3481 tgctgcctga aattgtgtgg aatttatgtt tgtaggaaaa ggtgcgtgtg gcaccgaggc
     3541 ttagggaggc gattaggggg ttggggccga gttcggcttc gtcgtcggcg tgccagccta
     3601 cgtagtcatc gccatttctg taccaattga cgagcacgga attaaattcg cagtttagat
     3661 aggtttgcac gcagtggcgc aagcgcgtta aaagtggcgt ccaggggcgg gttttgagta
     3721 ggttattgct gtagctgtag tggatacctg ggtcggcgtg ccaggtttgt ttgcgcggaa
     3781 gggtaaatgt gcggcctgct agactgtagt ggttatcggg ccattgctgt tgagatacga
     3841 gggatgcgta gagcgcggcg ctttcttcag ggctgtaaaa atgttggatg cactcaatag
     3901 ccggatgcgc ctcatcatgc agcggcagtt gggaaccggc taccggcgca ctcatagggt
     3961 taattggcta agttcggcgc gtttatcttt aattagatgc actaacgcgg caaaatcgct
     4021 tatggccagc tgaaaaatca cataggcgtc gtcgctatct ttgcgtttga agtcgtttat
     4081 ttccagcaaa aagcggttaa acattttaaa gatgcgatcg agggtcatca taaaccaggt
     4141 gtcgttatca atccatttat cgatataatc atctaaacgg ctcaccgccc atttaagcat
     4201 ggggaagctg gttttaaatt ctttaaaggt ttttaattcc tggtagtgct cgtagatttg
     4261 ttccaccgtc gggcgggctt tgctggcttc ttttaaaaag tttgccgagt aattagcaaa
     4321 taaattgtcg aactgggcct caaaggcggc atgggaatcc accgcgagcc agggtaaaaa
     4381 gttatcttct attacgctgt aacagttgct tagattggcc ataatgccat taaagctggc
     4441 gacaaaatcg ttttttccca attgggtttg tatagcttta gtgacggaca ccagttcaat
     4501 gatggtggcg ctggcgctgg ccagctcttt ggtaacggaa aactctaacc ccataacaca
     4561 gcctctttca ccctgcgttt aaaccctgag caaggccaca ggcctcgcca cgtgtattgc
     4621 caccagaata attcaagcag ggcttgccct gacttgtttt atgcattttg cgcggttaaa
     4681 tcgtcaagct cggcaatgca ggtattgtct tttacctggt gcaaggcgtt atcaaacgcc
     4741 atggcggagg tgaataattg gcttttagcg atcacttggc cgccggggtt tttaatataa
     4801 aaaaaggtat cgccgctggg ggttttacta gcgccgattt ggttgctcat taatgtgccc
     4861 acacggattt ctttaatggc ggcttcggct tcgtcccggc tggggtgggt gccgccaatt
     4921 aataataatt caccgttggc atctactagg ttgacaaaaa atcggttggt ttcatcgctg
     4981 gtcaataaaa atcgtgcaga cataattcac cttcagctct tggttgattg tgagtgctca
     5041 gcaattgggg tgccaggtgt atttatatcc ggcgcggaaa ttttttagct tttgggcgat
     5101 gggttacaaa caaataactg tgcagcgccc cacctcctgc ggggacgcat caagcggcaa
     5161 tgtcactagg tttataattt taacgtgttt tacagggtac tttgctccct gcctaaaacc
     5221 ccctcccaac ctccccctta aaagggggag gagcaaaaaa ccggcgtata aggcattttg
     5281 ctctcacgta ttgttaccca ttgtgggagc agggtgcaca gcgaagctgc ctgtaaggca
     5341 catcacaaag aaatgatgtg cgcgcggagg gttggagagg gaattacgga tgaatgcaca
     5401 atcacataaa tagcagtaca aaactgctca ttaaacttaa aaaccgcagt ttttaggttt
     5461 aatgacctgg ctcccgtagc aaaggtattt taagctccgc tcgaacccaa cactatcagc
     5521 cagcttttta gatgtgtatg ttgtcgttta aatgtgcatt ttgtcggcaa tgcgacaatc
     5581 ataattgtag ggcttctgct acagctttgt gggtgataac gccatcggca atgttcaggc
     5641 ctgctgcaag atgtgggtct ttttgcaggg cggcttgtac tccctggttg gccattttta
     5701 cgataaatgg cagggtggcg ctgttcagag ccagggttga ggtgcgggca acggcgctgg
     5761 gcatattggc tacgcaataa tgcactatgc cttcttcaat aaatataggg gcttggtggg
     5821 tggtggcgtg ggaggattca aaacaaccgc cctggtcgat ggcgatatcc actaaaaccg
     5881 ccccaggcaa taaagttttt aattgctccc ggcgaattaa tttgggcgcg gcagcaccgg
     5941 ggattaatac tgcgccgata attaaatcgg cggtttttat ggcttctgcc accgcttgag
     6001 cggagctaaa ggcggttttt acccggccgt taaacaattg gttaatttca attaatttgt
     6061 gggcggaaat atcaaacagc gttacatccg cgtgcaggcc cacagccatt tgtgcggcgt
     6121 taccccccgc cacaccgccg cctaaaataa tgactttagc ggggtttacc cccggcacgc
     6181 cgcctaataa aacaccccgc ccaccgtaag cggtgtgtaa ataattggcc cccacatgca
     6241 ccgcaagcct gcctgccact tccgacatgg gccgcagcag cggcaggccg ccttggggtt
     6301 cggtgacggt ttcataggcg atacagcggg cttttgattg cataagggca agggcttggg
     6361 gtttatccgc cgccagatgc aaataagtaa atagtgtttg ctggggttga attagcgcgc
     6421 attcgtgtaa ttgcggctct tttactttga cgattaattc cccggctgca aaagcacttt
     6481 tagcatccgg cactatttgc gcaccggcgg ctaggtattc ggcgtcgcta aagccgatgc
     6541 gctcgcccgc ctgagtttcg accaatactt catggccctg gcgagttaat tcagctacgg
     6601 aggttggcac cataccgacg cgg
//
LOCUS       DAOQGS010000191         6584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5741_length_6584_cov_4.576964, whole genome shotgun sequence.
ACCESSION   DAOQGS010000191 DAOQGS010000000
VERSION     DAOQGS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5741_length_6584_cov_4.576964"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKZ52_08685"
     CDS             <1..876
                     /locus_tag="PKZ52_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNG59889.1"
                     /translation="LFAQLLVDFTVFIGQEKRRIALCERRLVEAEDAKAVAEKARKRV
                     SVEVGLRMDGFILSEPLRDFIANVWFQVLFVRCLRAGNDSLPWRDALQTLTDLVWSVQ
                     PLKTAKDRQALIMMVPNLMERLRAGLDDIAYDPFATADICSLINRLHRDAVAAYNPAK
                     TQDQPPVAKSAPSAPPSASDETGPDDLHWQQVATFAPGSWFDIIEPGQPVLRCRLAAV
                     IKQTGSFIFVNRSGVKVAERQHYQLALGLRSGQLRPLDSSLLFDRALEEVVAGLRKTS
                     KSPLDMPAPKTDKKD"
     gene            complement(938..1261)
                     /locus_tag="PKZ52_08690"
     CDS             complement(938..1261)
                     /locus_tag="PKZ52_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59890.1"
                     /translation="MSTKNSVGELSFLRTVSNEANPHSGYHLAVKIKQDINFLQDRIE
                     RVKRLHTPNSSVLRTYESMLSTRLAVLDWMRDNGQLDELEETPAAQPSGNASLGKRAN
                     KDKTG"
     gene            2007..2708
                     /locus_tag="PKZ52_08695"
     CDS             2007..2708
                     /locus_tag="PKZ52_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59891.1"
                     /translation="MVETTSLFDLVLTGQLAQGVERASAVAQLALLFKMPAEKIEGLL
                     ASAPVVLKRDLGWEVAKRYRVAIKQAGALSDIRPAESVRPEAPVAPVASAPAPSPVPV
                     AAASAQPAAVADGENWSLAAVGADVLRPDERAAPVSRLDAFSDFSLRPNDGNLLDSSE
                     MAPLITAPKELGAQLDLLPEGTAVLAEHERALPLAALVDAPEYEVANLGERLSPPLTH
                     SAQAPDTSHLTLVKS"
     gene            complement(3102..4277)
                     /locus_tag="PKZ52_08700"
     CDS             complement(3102..4277)
                     /locus_tag="PKZ52_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNG59892.1"
                     /translation="MSTPIDALLHILAKKDGSDLYLSTGAPPCAKFQGTLKPLASEPY
                     KAGEVEAIAMAIMDEEQRKEFARELEMNLAISINNVGRFRINIFKQRNEISIVARNIN
                     TEIPQFDNLGLPEILKEVVMAKRGLLLFVGGTGSGKSTSLASLIDHRNSTAGGHIITI
                     EDPVEYVHKHKRCVVNQREVGVDTRSFKAALKNTLRQAPDVILIGEIRDRETMEHALE
                     FAETGHLAISTLHANNANQALERIINLFPEERRPQLLMGLSQNIRAIVSQRLVPTKEG
                     KRCAAVEVLLGTKTIAELILKARLTDIKEIMEKSENIGMQTFDGALFKLYKADKITFE
                     DAIANADSPNNLRLRIKLAEGKSVDGIDAGAAGTGASFGTLSLSMVEEEKPDDAASH"
     gene            4677..5063
                     /gene="cheY"
                     /locus_tag="PKZ52_08705"
     CDS             4677..5063
                     /gene="cheY"
                     /locus_tag="PKZ52_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNG59893.1"
                     /translation="MDKNMKILIVDDFSTMRRIIKNLLRDLGFTNTHEADDGVTALPM
                     LRSGDFAFLVTDWNMPGMTGINLLKEVRADPKLVALPVLMVTAEAKRDQIIEAAQAGV
                     NGYVVKPFTAQVLKEKIDKIFERVGG"
     gene            5092..5883
                     /locus_tag="PKZ52_08710"
     CDS             5092..5883
                     /locus_tag="PKZ52_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNG59894.1"
                     /translation="METPISQHNGEAFVAELKQCANLLVSKLQNDDFEDASALIHSLV
                     EVRDRHIFNSVGQLTRALHNAIVNFNVDASVHGKSDMVPSQIRDASDRLQYVISMTQE
                     AAEKTMDRVEASAPIAMSLGQEAAILRKEWEKLRRREISKTEFSGLYDRMSDFLEQMN
                     LGAEQLNDHLQAIILEQGFQDLTGQVLKKVIGLVTNVEKELVNLMRIAGQVEEVTGLD
                     VRTDAKEAQVVNRTDAEGPQIHAAKRDDVVQGQDDVDDLLSSLGF"
     gene            5894..>6584
                     /locus_tag="PKZ52_08715"
     CDS             5894..>6584
                     /locus_tag="PKZ52_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNG59895.1"
                     /translation="MSFGEDDDILQDFLVEAGEILDKLSEQLVDLEQRPNDRDLLNAI
                     FRGFHTVKGGAGFLQLDAMVACCHITENLFDILRNGQRTVTSELMDVVLQALDTVNAQ
                     FAAVSARQDAMPASPELIARLERLVSCADLAEPAPVAAPAPQAAAAPVAAPSEPEGSG
                     INLSDDEYEGLLQAISGDKSAPAPAPKKAVEEPITAAASASEDISESEFESLLDQLHG
                     AGKGPGAASQSR"
BASE COUNT         1532 a         1535 c         1805 g         1712 t
ORIGIN      
        1 ttatttgccc aattgttagt ggattttacc gttttcatcg gccaggaaaa acgcaggata
       61 gccctgtgtg agcggcgttt ggtggaagcg gaggatgcca aagccgtggc ggaaaaagcc
      121 cgcaaacgtg tgtcagttga agttgggctg cggatggatg gctttatttt aagtgagccc
      181 ctgcgcgatt ttattgccaa cgtgtggttc caagtgttgt ttgtgcgctg cttgcgcgca
      241 ggcaatgata gcttgccctg gcgggatgcc ctgcaaaccc tcacggattt agtctggagt
      301 gtgcagccgc tgaaaactgc taaggatcgt caagcgctga ttatgatggt gccaaattta
      361 atggagcgcc tgcgcgcggg tttggacgat attgcctatg atccgtttgc cacggccgat
      421 atatgctcct taattaaccg cctacatcgc gatgcggtgg cggcctataa tcccgctaaa
      481 acccaggatc aaccccctgt tgccaagagc gcacccagcg cgccgccatc ggctagcgat
      541 gaaaccggcc cagatgactt gcattggcaa caggttgcca cctttgcacc gggttcttgg
      601 ttcgacatta ttgagccggg ccaaccggtg ttgcgctgcc gcttagcggc ggtaattaaa
      661 cagacgggca gctttatttt tgttaaccgc agtggcgtta aggtcgccga gcggcagcat
      721 tatcaattgg cccttgggct gcgctcaggc cagctgcgcc cactcgatag cagcttatta
      781 tttgatagag cattagaaga ggtggtggca ggtttgcgca aaactagcaa atcacctttg
      841 gatatgcctg cgcctaaaac ggataaaaaa gattaaacag tcgttgcgcg cagcgcactg
      901 ctcatatgta tttacccagc caagtacagg cccccagtca gccggtttta tctttattag
      961 cccgtttacc taggctggca tttccgctgg gttgcgcggc gggagtttct tctagttcgt
     1021 ctagttgccc gttatcgcgc atccagtcta aaacggctaa gcgtgtggag agcatcgatt
     1081 cataggtgcg cagcacgctg gagttaggtg tgtgcagccg tttaacgcgc tcaatgcgat
     1141 cctgcaaaaa attaatatcc tgctttattt tgactgccaa atggtatccc gagtgcgggt
     1201 ttgcctcatt actgaccgtg cgtaaaaaag ataactcgcc gacggaattt tttgtgctca
     1261 tgccctacgt ccccgtgatg tatcgccgat ggtcaatgtg ctcgcgtgta taaagcctct
     1321 gcgcgttgac aggcgttttt gtgccgttta cctactatta aaatcacatt taattgttat
     1381 ctaaatgggt taaatattta tgacgacggc ataccctgtg tgctgtccta tgcgtgagta
     1441 atcaattatt gcttcactag agtatggaac tatttttagc ctgcgctagt ttatttgcgc
     1501 ccgcagggtg ttgcttccgt caacagggtt agcagttgcg tgcataaaaa attgtgcgtg
     1561 gaaaagcgcc ataccagttt aacctgccaa tgctcgactg gcccaaggcg taaggtattg
     1621 caaatttgta cgggcattca gtaggttaaa tggccatagc ggtgtggggt aaccaacctg
     1681 tcaaagcctt ggcgctattt ggaatcgcta taaataaaaa cggatttccc ggtggttaat
     1741 cggttttaag aaataaacaa tctgatcgtt ggcgcttatt ccgctcgctt gcaccaggtg
     1801 gactctacac atgtttgtat tgctgcacat tgagatacac ttaaggagcc gctgattaag
     1861 tcttgggggc ggggagattg tgcaccctgc gcaggttttt tgcgcgaagg cctaaaaggc
     1921 ctaagaggaa gcaccctgtg gccgaatcgg gctataagtg agtggcaatt ttaaataaca
     1981 aacgcacagc tgtattagag gcggttatgg tagagacaac atcgctattt gatttagtgc
     2041 ttacgggcca gcttgcccaa ggggtggagc gcgcaagcgc agtggctcag ttagcgctgc
     2101 tttttaaaat gccagccgag aaaatagaag gccttttggc cagtgcgccg gtggtgctga
     2161 aacgggattt gggatgggaa gtggccaagc gttaccgcgt tgccattaaa caggccggtg
     2221 ccttaagtga tatacggccc gcagagtccg tgcgccctga ggcgcctgtc gcacccgtcg
     2281 catcggcacc ggcgccttcc ccggtgccgg tagccgcagc ttcagcgcaa ccggcagctg
     2341 ttgctgatgg agaaaactgg agtttggccg ccgttggggc ggatgtattg cggccagatg
     2401 agcgcgctgc gcccgtgtct aggctggacg catttagcga tttttccctg cggcctaatg
     2461 acggcaattt attagatagt tctgaaatgg cgccattaat taccgccccc aaagaattgg
     2521 gcgcgcaatt agatttattg ccagagggta ctgcggtgct ggcggagcat gaacgggcgc
     2581 taccgctggc ggctttggtt gacgcgcccg agtatgaggt ggcgaaccta ggtgagcgat
     2641 tgtcgccgcc gctgacccat tcggcacagg cgccggatac ctcccatttg accttagtta
     2701 aatcctaaaa ccgctgcaga atttcaccca agttttaccc caggcggcca taggccgcct
     2761 tttttatcgg tgagggtatt tcctgcgctg acatttgcat actttgtgta attttgcgca
     2821 agctatttag gcgacgataa tcaggtctag atatgtactc gtacgcttgc acaatctgca
     2881 aaaagtttaa ttattcagta cctttttatt cagcagtttt tatgcagcac tttatgtttt
     2941 agctggacgt gatacctacg ccgcctagaa actaccctgt gagtgtttta gtactgcgag
     3001 ccctcaatac tcctcctgcc ttgagttgaa cttttgcaaa aaaaagtgag cagatgcgag
     3061 ccctaggtgg taatcgtaga gttcaactgc tgtttttagg tttaatggct ggccgcatcg
     3121 tcgggttttt cttcttccac catacttaat gacagcgtgc cgaagcttgc gcctgtgccc
     3181 gctgcgccag catcaatacc atccacactt ttgccttcgg caagcttaat gcgcaggcgc
     3241 aaattattgg gggagtcggc gttggcgatg gcatcttcaa aggtgatttt atcggcttta
     3301 taaagtttaa atagcgcgcc atcaaaggtt tgcatgccga tattttccga tttctccatg
     3361 atttctttta tatccgtcaa gcgcgctttg agaattaatt cggcgatggt tttcgtgccg
     3421 agcaatactt caaccgccgc gcagcgtttg ccttctttgg tgggcaccag gcgttgggac
     3481 acaatagcgc ggatgttttg cgacagcccc atcaacagct gtggcctgcg ctcttcaggg
     3541 aataaattaa taatgcgctc aagcgcttgg ttggcgttat tggcatgcaa agtggaaatg
     3601 gccaggtggc cagtttcggc aaattctaag gcgtgttcca tggtttcgcg gtcgcgaatt
     3661 tcaccgatta aaatgacatc cggcgcctga cgcagggtgt ttttcagcgc tgctttaaaa
     3721 ctgcgcgtat ccacacctac ttcccgctga ttcaccacgc aacgcttgtg tttgtgcaca
     3781 tattccaccg ggtcttcaat ggtaataatg tggccgcccg ccgtgctgtt gcgatggtca
     3841 attaaagacg ctaaagaggt ggatttacct gagccggtgc cgccgacaaa taacagtaaa
     3901 ccccgtttgg ccatcactac ttcttttaaa atttccggca ggcctagatt gtcaaattgg
     3961 gggatttccg tattgatatt gcgggcgacg atggaaattt cattgcgttg cttaaatata
     4021 ttaatacgaa agcgaccgac attattaatg gaaatagcca ggttcatctc cagttcccgc
     4081 gcgaattctt tgcgctgctc ctcatccata atcgccatgg caatggcttc tacctcaccg
     4141 gctttataag gttctgaagc taagggtttt aatgtgccct gaaattttgc gcagggcggc
     4201 gccccggtac tcaggtaaag atccgaaccg tctttttttg ccagtatatg taaaagggca
     4261 tcaatggggg tgctcatggg gggctccttg gtgagcgcag tgcaaaggcc taggctgtag
     4321 gggatgttta gttgagagta tagaaggcct gcttatacct gcaattgagg ataaaccccg
     4381 gcaaagcgca ttgggtggtc tatgctgtaa atcgttaggg ggtatttttg caactgactg
     4441 ctaaatgcag gcggccccca gtaacgtaat tttgacgtct gttgaatggg ttaaaaactg
     4501 gctgactgag ccttaacagc tagacttgct gtaaaggaag ctctacgggc tatttgcaaa
     4561 actgctgcag gcgaggaggc ctggatggcg ctgcgggacg cggctgggtg gttttgcgca
     4621 gacggcttaa tgcagaggtt tctacaccca gtggcaagaa tattttggag gttgcattgg
     4681 acaagaatat gaaaatcctg attgtcgatg acttttcgac aatgcgtcgg atcataaaaa
     4741 atctgctcag ggacttgggt tttaccaata cccacgaggc cgacgatggt gtaaccgcat
     4801 tgcccatgct gcgcagcggt gattttgcgt ttcttgtaac ggattggaat atgccaggca
     4861 tgacaggaat taatttatta aaagaagtcc gcgccgatcc caaattggtg gcactgccgg
     4921 tgctgatggt gacagccgaa gccaagcgcg atcaaattat tgaagcggcg caggcgggtg
     4981 ttaatggcta cgtggttaaa cccttcaccg ctcaggtatt aaaagaaaaa atcgataaga
     5041 tttttgaacg tgttggcggc taataaaaac ataatggcgg aagggtatcc gatggagact
     5101 ccgatttcac agcacaacgg cgaggccttt gtggccgaat taaaacagtg cgccaatctg
     5161 ctggtaagca agttacaaaa tgatgatttt gaagatgcat cagccctgat ccacagcttg
     5221 gttgaagtgc gcgaccggca tatttttaat tccgtaggcc agctgacacg ggcgctgcac
     5281 aacgccattg tcaattttaa tgtggatgcc agcgtgcacg gtaaaagtga catggtgccc
     5341 tcgcaaattc gcgatgcctc tgaccgcttg caatatgtga ttagcatgac ccaggaggcg
     5401 gcagaaaaaa ccatggaccg cgtggaggcg tctgcaccta tagccatgag tttggggcaa
     5461 gaggcggcaa ttttgcgcaa agagtgggaa aaactgcgcc gccgcgagat ttccaaaacg
     5521 gaattttccg gcttgtatga tcgtatgtcc gattttttag agcagatgaa tttaggtgct
     5581 gagcagttaa atgatcatct gcaggccatt attttggagc agggatttca ggatttaacc
     5641 ggccaggtac tgaaaaaagt cattggcctg gtgaccaatg tggaaaaaga attagtcaat
     5701 ttaatgcgca ttgcgggcca agtggaggag gtcacgggcc ttgatgtgcg cactgacgca
     5761 aaagaagcgc aggtggtaaa ccgcaccgac gcggaggggc cgcaaataca cgccgccaaa
     5821 cgggatgatg tcgtacaggg gcaggacgat gttgatgatt tactctcaag cctaggcttt
     5881 taaggagcgc tcgatgtcgt ttggagaaga tgatgatatt ttgcaagact ttctggttga
     5941 ggccggtgag attcttgata agttatccga gcaattagtt gaccttgaac agcgccccaa
     6001 tgatcgggat ttattaaatg ctattttccg cggctttcac accgtaaaag gcggtgcggg
     6061 ctttttgcaa ttagatgcca tggtggcctg ctgccatatt acggaaaacc tttttgatat
     6121 tttgcgcaat ggccagcgca cggttacgtc ggaattaatg gatgtggtgc tgcaggcgct
     6181 ggacaccgtt aacgcccagt ttgccgctgt ttctgcccgc caagatgcca tgccggccag
     6241 cccagaatta attgcgcgct tggagcgctt ggtgagctgt gcggatttag cggagcctgc
     6301 gcctgtggcg gcacctgcac ctcaggctgc agcagcacct gttgccgccc ccagcgagcc
     6361 tgaaggcagc ggtattaatt taagtgacga tgagtatgag ggcctgttgc aggccattag
     6421 cggcgataaa tctgccccgg cgcctgcgcc taaaaaagcg gtcgaggagc ctattacagc
     6481 ggccgcttct gcctctgagg atatttccga aagtgagttt gaatcgctgt tagatcaatt
     6541 gcacggcgcc ggtaaagggc cgggtgcggc ctctcaaagc cgct
//
LOCUS       DAOQGS010000192         6570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5757_length_6570_cov_5.374981, whole genome shotgun sequence.
ACCESSION   DAOQGS010000192 DAOQGS010000000
VERSION     DAOQGS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_6570_cov_5.374981"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKZ52_08720"
     CDS             <1..513
                     /locus_tag="PKZ52_08720"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNG59896.1"
                     /translation="VVEHKQVQLDLRSIGGSALTDAAIAVPEDSPSGIPVTYVPARNT
                     IFLSIALGWAEVLEADAIFIGVNAVDYSGYPDCRPEFIDAFARLAQLATKAGVEGRPI
                     EVKTPLLHLSKADIIKQGTALGVDYGQSVSCYQADAEGRACGRCDSCRLRRKGFLDAQ
                     VPDPTRYQSA"
     gene            685..1371
                     /locus_tag="PKZ52_08725"
     CDS             685..1371
                     /locus_tag="PKZ52_08725"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNG59897.1"
                     /translation="MKAAAPFLILSLFFCDLALADGKAVISAGGRTATLGAGVKSSGG
                     SSIKVFKYRDKKGVASFSDAAPVDAAYEVLTYGCFACNVNSSVDWYTTRLHTDYSDHI
                     RVASEAYAVDPALVRAVIHAESGFNPMARSGKGAVGLMQLMPGTARDMGVSNSYEAGQ
                     NIMGGARYLSYLLGELKGDVALATAAYNAGLANVTKYQGIPPFAETQAYVKRVGILYE
                     RYKSQKLAKL"
     gene            complement(1484..2032)
                     /locus_tag="PKZ52_08730"
     CDS             complement(1484..2032)
                     /locus_tag="PKZ52_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59898.1"
                     /translation="MSFKYLGALLLAVLSPFCLAEDGKAQAHTFDDLYRSLCLKNINN
                     LNKLKEQLALSPQMAPEQTRQILRGAKGFAWRIPSDEGAFILTLQDNTPTCTIMAHHA
                     DAKTVEELFLKLVNAPIAPLESKKISSREDKTKANGTAHTLTYEWQAKQGKHKLVFVL
                     STSSYEKADLQAFGSISLVTEQ"
     gene            complement(2097..3008)
                     /gene="rdgC"
                     /locus_tag="PKZ52_08735"
     CDS             complement(2097..3008)
                     /gene="rdgC"
                     /locus_tag="PKZ52_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNG59899.1"
                     /translation="MWFKNLRIYTLSSPFTLKPEALSQALEAQAFKPCGNLDPMRYGF
                     VPPLGRHGVELVHVTNGNIMVCAKKQEKIVPGGVVKDALEEKISEIKAQGRSVGRKER
                     QELKDEILFSLLPRAFSRTRLDFAYFDVQKHWLVVNAASAKRAEELIQALRLALGTLP
                     CVPLAAEINPVLQMSQWLGQGFADAPFVLGEECELNAPKDGRVVRVKKQDLSADEIRN
                     HLTVGLRVQKMALHWKDAISFVLDEQLAVKRLKFNDVLSEQADDRRAESAAEEFDASF
                     AIMALAVRDFIAELLAALGGQAPVDQL"
     gene            complement(3020..3646)
                     /locus_tag="PKZ52_08740"
     CDS             complement(3020..3646)
                     /locus_tag="PKZ52_08740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014701238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNG59900.1"
                     /translation="MERPMSLDSIEQQISYGIGRQMGDQLLGNNIPGIDVAAVCAGIS
                     DALAGTPSRLSQEQLQTAFGILNGRLQEQQHREAKQQAAAGEQFLAHNRTRPEVITLD
                     SGLQYEVLKQGDGAKPSASDTVSTHYHGTLIDGTVFDSSVERGAPAQFPVNRVIAGWT
                     QALQLMPVGSKWRLYIPYQLAYGERGAGGAIAPFSALIFDVELLSIVN"
     gene            complement(4426..4911)
                     /locus_tag="PKZ52_08745"
     CDS             complement(4426..4911)
                     /locus_tag="PKZ52_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNG59901.1"
                     /translation="MSISKDQVVSVHYRVSEAGGPELEHNFDTIPMVYLHGHNNLMPA
                     LEKALEGKNVGDTLEVTLAAADAYGPYIEGATQRVPIKNLLNKPKRLLPGGLVQLNTN
                     QGVASARVLKVGKFNVDVDLNHPFAGKDLAFYLEVKDVRPATEEELAHGHAHGVGGHQ
                     H"
     gene            5041..5604
                     /locus_tag="PKZ52_08750"
     CDS             5041..5604
                     /locus_tag="PKZ52_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HNG59902.1"
                     /translation="MYPSDPQKAVEHWLQEVVIGLNLCPFARKPFMAGKVRIVAVDSP
                     RAALISHLQEELNYLTAHEEVDTSILVLTQALDDFLAYNDFIHEVEDWLWQQGSEGIY
                     QVASFHPDYYFADSEPNDVSNFTNRAPFPLLHILREQQLEELLARYPDADEIPENNIR
                     RLRQLSAEQLRGLFSYLIPRGHFAFLG"
     gene            complement(6027..>6570)
                     /locus_tag="PKZ52_08755"
     CDS             complement(6027..>6570)
                     /locus_tag="PKZ52_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HNG59903.1"
                     /translation="WIGKDLDKLWLKTDLTQTAGTLEEAEVQALYSRAVSAYWDLQLG
                     LRHDAKPSPSQDWLVIGVQGLAPYWFDVDAALFIGSAGQTSARLTAEYELLITQQLIL
                     SPEVGLTFYGQNDPSREIGAGLAEATASLRLSYAFWREFAPYVGINWRGHYGNTAEFI
                     RQHGEKAQQTEGVIGLQAWF"
BASE COUNT         1376 a         1760 c         1896 g         1538 t
ORIGIN      
        1 gtggttgagc acaaacaggt gcagctggat ctgcgcagca tcggcggctc cgccctcact
       61 gatgcggcca tcgccgtgcc ggaggattcc cccagtggta tccccgtcac ctatgtgccc
      121 gcccgcaata ccatctttct ctccatcgcc ttgggctggg cggaggtgct ggaggcggac
      181 gcaattttta tcggcgtgaa tgccgtggac tactccggct acccggactg ccggccggag
      241 tttatcgacg ccttcgcacg gcttgcccag cttgccacta aggccggggt ggaagggcgg
      301 cccatcgagg tcaaaacgcc tttgctgcac ctgtctaaag ccgacatcat taaacagggc
      361 accgccctgg gggtagatta cggccagagt gtttcctgtt atcaagccga tgccgagggc
      421 cgcgcctgtg gccgttgtga ttcctgccgc ctgcgccgca aaggtttttt agacgcccaa
      481 gtgccggatc ccacccgcta tcaaagcgcc taacaaccca tgcgccggtt cacactgggg
      541 tgggcatcta gtactaattt ctcaacttaa gcctggcaag catggctgcc ccgcttgttc
      601 gctgcgcact cctgccctag actcaaccga ggcaaaccct tcgggtggcg ggagactttg
      661 gcagaacaat aaagagagtc cagcgtgaaa gcagcagccc cttttctcat cctgagttta
      721 tttttctgcg acctcgccct ggcggatggc aaggcggtga tcagtgctgg ggggcgcacg
      781 gccactttgg gtgcaggcgt taaatcctcc ggcggctcct ccatcaaagt gtttaagtac
      841 cgggacaaaa agggcgtcgc gtccttttcc gatgcggcgc ctgtagatgc tgcctatgaa
      901 gtgcttacct atggctgctt tgcctgtaac gtcaattctt ccgtcgattg gtacaccacc
      961 cgtctgcaca ccgactacag cgatcacatc cgcgtggcct ccgaagccta tgcggtagac
     1021 ccagccctgg tgcgggcggt gattcatgcc gaaagtggct ttaatcccat ggcccgctcc
     1081 ggcaagggcg cggtgggttt gatgcaattg atgcccggca ccgcgcggga tatgggggtg
     1141 agcaattcct atgaggcggg gcagaacatt atgggcggtg cccgttatct gtcttatctg
     1201 ctgggtgagc taaaaggcga cgttgccctg gccactgccg cgtacaatgc gggcctggcc
     1261 aatgtcacca aatatcaggg cattcccccc tttgcggaga cccaggccta tgtcaaacgg
     1321 gtggggattc tctacgagcg ctacaaaagc cagaagctcg ctaaactcta agcccacctc
     1381 gtttatgggc ttggagtgcg ccgccgccct ccaagcgttt cctgtcttaa acctataaac
     1441 cgccgtagca gagttcaacg gcggtttata ggttagatgt ctcttattgc tcggtcacta
     1501 acgaaatact gccgaaggcc tgcagatcgg ctttttcgta gctggatgtg ctcaatacaa
     1561 aaaccagttt gtgtttaccc tgcttggcct gccattcgta ggtgagggtg tgcgctgtgc
     1621 cgttggcttt ggttttgtct tcccggctgc tgattttttt ggactctagg ggggcgatgg
     1681 gggcgttgac gagttttagg aatagttcct caacagtttt ggcatcggca tggtgcgcca
     1741 taatcgtaca ggtaggcgtg ttgtcttgga gggtaaggat aaatgcgccc tcgtcgctgg
     1801 ggattcgcca ggcaaaaccc ttggcgccgc gcagtatttg gcgagtctgc tcgggtgcca
     1861 tttgcgggct aagcgccaac tgctctttga gcttatttag gttattgatg tttttaaggc
     1921 acaaggacct gtacaggtca tcgaaggtgt gcgcctgggc cttgccatct tctgccaaac
     1981 aaaagggaga gagcactgct agcaataacg caccaaggta tttgaaggac ataattcact
     2041 cactattgaa ggaaaataaa caaacccccg gtgcacaaat ggcggtgggc ttatgactag
     2101 agctggtcaa ccggcgcctg gccgcccagg gccgccagca actcggcaat aaaatcccgc
     2161 accgccaggg ccatgatggc aaaggaggcg tcgaactcct cagcggctga ttcggctcgc
     2221 cggtcgtccg cctgctcgct gagcacatcg ttaaacttca ggcgtttgac ggccagttgt
     2281 tcatctaaca caaaactaat ggcgtctttc cagtgcaggg ccattttttg tacccgcagg
     2341 cccacggtta agtggttgcg gatttcatcg gcgcttaaat cctgcttttt aacccgcacc
     2401 acgcggccgt ccttaggggc gttaagttcg cattcttcac ccaacacaaa cggcgcatcg
     2461 gcgaaacctt ggcccagcca ttggctcatc tgcagcaccg ggttgatttc ggctgccagg
     2521 ggcacgcagg gcagggtgcc gagggctagg cgcagggctt gaattagctc ttcagcccgt
     2581 ttggcggagg cagcgttgac caccagccag tgtttttgca catcaaaata ggcgaaatcc
     2641 aagcgagtgc ggctgaaggc gcggggcagc agggagaaca aaatctcgtc tttaagctcc
     2701 tggcgctcct tgcggccgac gctgcggccc tgggctttga tttcgctaat tttttcctcc
     2761 agggcgtctt tgaccacgcc gccggggaca attttttcct gctttttcgc gcacaccatg
     2821 atgttgccat tggtgacgtg caccagctcg accccatggc gccccagggg cggcacaaag
     2881 ccgtagcgca tagggtccag gttgccacag ggcttaaacg cctgggcctc aagggcctgg
     2941 cttaaggcct caggcttaag ggtgaagggg ctgctgagcg tgtatatacg caggttttta
     3001 aaccacatgc gcaggcttcc tagttgacga tggacagcag ttcaacgtca aaaatcagcg
     3061 ccgagaaagg ggcgatggcg ccaccggcgc cgcgctcgcc ataggccagc tggtagggaa
     3121 tatacaggcg ccatttggag ccgacgggca ttaattgcag ggcctgggtc caaccggcga
     3181 tcacgcggtt aaccgggaat tgggccgggg cgccgcgctc cactgagctg tcaaacacgg
     3241 tgccgtcaat tagggtgccg tggtagtgtg tgctgacagt gtcggaagcg ctgggtttgg
     3301 cgccgtcgcc ctgcttgagt acttcgtatt gcagtccgga gtcgagggtg attacctctg
     3361 ggcgggtgcg gttgtgggcg agaaattgct cgccagcggc ggcctgttgt ttggcttcgc
     3421 ggtgttgttg ctcctgcagg cgcccattga gaatgccaaa cgcggtttgc aattgctcct
     3481 ggcttaaacg gctgggtgta cctgccaggg cgtcgctgat gcctgcgcag acggcggcca
     3541 catcaatgcc gggaatattg ttgcccagca actggtcgcc catttggcgg cctatgccgt
     3601 agctgatctg ttgttcgatg ctgtcgaggg acatagggcg ctccaaaaaa gcggcgagtc
     3661 taccagcctt aagggcgatg cgctcggcag tagggcgaat ttgtcgaatg gtttgccccg
     3721 catctgcttg gcgtcaaaat ccaactgctg cggttgaaat agatcccaaa aaaattaatc
     3781 cacccacttg ctttttgttg cgactttagt gggaaaagcg ctgtgcagtc tgtccacaga
     3841 atctgtggat aacttagtgg atagcatttg agaaacaggg gtaaaggccg aaaatcgctg
     3901 gcctgggggg tgctggttat tttttagtct aataaaaaat acaaataaaa tcaataatat
     3961 ataaatgtct agccctgtaa ttaaaaaaat gacacaaaaa aagtgcttga cgatggccaa
     4021 taatctggcg cacagggatt gtgcataaca ttttgccgtg aaacataatt tcacccgccg
     4081 gggccgttta aattgtgccc taccgatggc tcgatggcgc ccgctaaagc gtgaaaaatc
     4141 cgggttttag tcagtcaaaa gtggccgcca cgtgatcgcc ggaagcgggt aacgcaggtg
     4201 gccagacgtg aaaccagact gaagctgcgc ctgccgcaac ggcaggcgtg aacaattcag
     4261 gtgcacagaa ggcaaaaagc cgccctaaac ccggatcggg cggccactaa gcagggacaa
     4321 ggtcagaagg agcggggcac aggggcgccc cttgtgccag gctcaactcc agaaattcac
     4381 aatttaagga attggcctgg gggcgctggg ggcctagagg ttggcttagt gctggtggcc
     4441 gccgacgccg tgggcgtggc cgtgggccag ttcttcttct gtggctgggc gcacgtcttt
     4501 tacttccagg taaaaggcca agtctttacc ggcaaatggg tggttcaaat ccacgtccac
     4561 attaaatttg cccaccttga gcacccgggc actggccaca ccttgattgg tattgagctg
     4621 caccaggccg cctggcagta ggcgcttggg tttattgagg aggtttttaa tcggcacccg
     4681 ctgggtagcg ccttcaatat aggggccata ggcatcggcc gccgccaggg tgacttccag
     4741 cgtgtcgcca acattcttac cctccaaggc tttttccagg gcgggcatta ggttgttgtg
     4801 gccgtgtaaa tagaccatgg ggatggtgtc gaaattgtgt tcaagctctg gcccaccggc
     4861 ttcactgacc cgatagtgca cgcttacgac ctgatcttta ctaatactca tggctggctc
     4921 cgcaaaaaaa gcggcgatta taacggcact cgcctgcgcg gtatgcagct gcaggtgtat
     4981 ttgtgcagcg gcccaggtcg caggttaaga tggccgccgt tttatccccg ggagtttttt
     5041 atgtacccct cagacccgca gaaggcggtt gagcattggc tgcaggaagt ggtcatcggc
     5101 ctgaacctat gcccctttgc ccgcaaaccc tttatggccg gaaaggtgcg catagtggcg
     5161 gtggattcgc cgcgcgctgc attgatcagt catttgcagg aagagttaaa ttatttaacc
     5221 gcccatgagg aagtggatac cagtattttg gtgttaaccc aggcgttgga tgattttctg
     5281 gcctacaacg attttatcca tgaggtagag gattggttgt ggcagcaggg ttccgaaggt
     5341 atttatcagg tggccagttt tcacccggat tattattttg ccgatagcga acctaatgat
     5401 gtgagcaatt ttaccaaccg ggcgccgttt cctttattgc atattttgcg cgagcagcag
     5461 ctggaggaat tattagcgcg ctacccggat gccgatgaaa ttccagagaa taatattcgc
     5521 aggttgcgcc aattatccgc tgaacaattg cgcgggttat ttagttattt aattccgcgc
     5581 gggcattttg cctttttagg ctaggcgaga ccgcactacc gctttagtgt cgacgcgtta
     5641 tttaacctaa aaaccgcagt tgaactctac tacggccgcc taatgcttga atctgcacgc
     5701 ttttttgtca aaatttgact caccgtagtg gtgactactg ctttcgccaa attttgacaa
     5761 aaaagcgccc atattcagcg ccttaggcac cctcgctacg agttcaactg cggtttttag
     5821 gtttaaatct cccccgcagt aataccggta gtcactaagg tggaagaagt atcgggggct
     5881 cgcagcacta aagctgtccg cccagttgtt ttcagccgtg cgggagttac tacctaggga
     5941 tataaaacgc agagtaaata atccgccgcg tccatgcagc ggcttaaatt tatagagcgg
     6001 cggactgggc cgcgcataga gtcctgttag aaccatgcct gcaggccgat aaccccctcc
     6061 gtctgctggg ccttctcccc gtgttgcctt ataaactccg ctgtattgcc atagtgcccg
     6121 cgccaattaa tgcccacata gggcgcgaat tcacgccaga aggcatagct caagcgcagg
     6181 ctggcggtgg cttcggcaag ccctgcacct atctcgcggc tggggtcatt ttggccataa
     6241 aaggtcaggc caacttctgg gctgaggatc agttgttggg tgattaacag ctcatattca
     6301 gccgtgaggc gggctgaggt ttggcctgcg ctgccgataa acagggctgc atccacatca
     6361 aaccaatagg gcgccagccc ctgcacaccg atcaccagcc agtcttggct ggggctgggt
     6421 ttggcgtcgt ggcgcaggcc cagttgcagg tcccaatagg ccgaaaccgc gcggctgtat
     6481 agcgcctgca cttcggcttc ctccagggtg ccggcagtct gggtcaggtc ggttttcagc
     6541 cagagcttgt ctaagtcttt gccgatccag
//
LOCUS       DAOQGS010000193         6523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5811_length_6523_cov_4.672233, whole genome shotgun sequence.
ACCESSION   DAOQGS010000193 DAOQGS010000000
VERSION     DAOQGS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_6523_cov_4.672233"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..567
                     /locus_tag="PKZ52_08760"
     CDS             22..567
                     /locus_tag="PKZ52_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG59904.1"
                     /translation="MRRRIRRNNEGSNIDITPFMNLMMILVPALLFNAVFTRINIIDL
                     TLPHVAGISGTAEELKDKKIEVIVRSDRLTVNFPAGVEVQTLPLVNGKQDSETLSLVL
                     QSLKKNLAGQGVDKKDIMILSEPNTDYQTIVSIMDSVRSYKTVVAASLVSTALFPEIA
                     LGDAPGSVEIPAPAVIPGGAP"
     gene            564..1085
                     /locus_tag="PKZ52_08765"
     CDS             564..1085
                     /locus_tag="PKZ52_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG59905.1"
                     /translation="MKTSMRAKRKYRKEKRKRPLVILNLVSLVDMFTILLCFLLVNAP
                     DGEVLENEYNVNLPESVSEVKPSGHLIITVTGEDIKVAGQSVMTVAQAIGDGQVILPA
                     LQQVLGAEATKTPYLTEEAKEKGRFITVMGDSAVSYALLKKIMATCAQSEYRNISLAI
                     NQIDAAPAAAGGT"
     gene            1085..2110
                     /locus_tag="PKZ52_08770"
     CDS             1085..2110
                     /locus_tag="PKZ52_08770"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNG59906.1"
                     /translation="MSALVSPNLLLPWANIPQENRRLNFALGLGALVFLVFFAIVKWV
                     ELPPPAKPAPVATTLMIVDQPVVLPPPPIPVEPPPPEPEKVEQKPEPKPEPKPEPKPE
                     PKPEPKPVKTEPVKTPVKEPTANNAEVKLDNARKQAASAGVMQAQNELAAMRDSLNIS
                     DVAKPTDATPSDNNAANTAATVDRAVISSGALAKSGGISVAALSKDTGGGGSLGGRKG
                     TAVENKLKDADQAAAAKQAAASAGGGKGSRTDEELKRILDAAKARITAVYYRALDDDP
                     TLQGRMVFKLTIEPSGAVSNVLVVSSALKNDDLQRKITALLRGLNFGARDVAVYTQTV
                     PIDLFPS"
     gene            2315..2665
                     /locus_tag="PKZ52_08775"
     CDS             2315..2665
                     /locus_tag="PKZ52_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNG59907.1"
                     /translation="MFVLKGVLRAESQEKILIYLLLRETGYAKNIAEFYGAPTNPIQK
                     QLLRLEEDGVVVGASLGKLKQYKLNPRYPFIEPLKSLLKKALEIYPPEVRVEGLVQRT
                     RPRKSGKPLQSLRK"
     gene            2706..3308
                     /locus_tag="PKZ52_08780"
     CDS             2706..3308
                     /locus_tag="PKZ52_08780"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNG59908.1"
                     /translation="MDFSHTPIIELARIIAEHLQDQGIEVVLVGGLAVEIYTENIYLT
                     KDIDMVNTNYASSRHLNTAMAQLGFAKKGRIYVNPTTDITVEFPTGPLSVGDELITRV
                     TVAQLATGQIPILQVSDIVKDRLAAYFHWRDRQSLVQALAILVKHPLKITSIKAFCNQ
                     ECAESFYPALKTLLNKARKQKLAQMEELELMVADYFIDEL"
     gene            3697..6303
                     /gene="leuS"
                     /locus_tag="PKZ52_08785"
     CDS             3697..6303
                     /gene="leuS"
                     /locus_tag="PKZ52_08785"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNG59909.1"
                     /translation="MTAISSEYTPHTIEPAVQDFWRDAKAFEVTEDASREKFYCLSMF
                     PYPSGRLHMGHVRNYTISDVIARYQRMLGKNVLHPMGWDAFGLPAENAAIKNKTAPAA
                     WTYSNTAYMKKQLTELGFGFDWSREITTCKSDYYKWEQWFFTRLYEKGLVYKKMASVN
                     WDPVDQTVLANEQVVDGKGWRSGAPVERKEIPQWFIRITDYAEELLADLDKLPHWPEQ
                     VKTMQRNWIGKSRGLEMYFKLEKPLGAHLGFDVYTTRPDTLMGVTYVSLAAQHPIALA
                     LAQTHSELAAFIDSCKVQSLAEADMANMEKRGMDTGLKALHPITGEPVALWVANYVLM
                     DYGTGAVMAVPAHDQRDFEFAQKYNLPINQVIAPLDGSAIDLSQGAYTEKGKLINSGE
                     FTGLDFAAAFDSIAATLEAQGRGRITTNYRLRDWGVSRQRYWGAPIPMFNLPSGGEIP
                     VPLDRLPVLLPEDVVMDGIQSPIKADPNWRQAEFNGQLVEHETDTFDTFMESSWYYAR
                     YTCPNFKDGMLDPAAANYWLPVDQYVGGIEHAILHLLYARFFHKLMRDEGLVKCDEPF
                     ERLLCQGMVNAETFFKKDSEGRITWVAPSKVKIDKDEKGRLIQAFHMETGDPLEFGGV
                     VKMSKSKENGVDPQEVINSHGADTIRLFSMFAAPPEQSLEWTDSGVIGAANFLKKLWR
                     AVHHHLAAGTPGTLDASSLNQTQKDLRRKTHETIKKVSDDFGRRQVFNTAIAAIMELL
                     NELGRHADRATPQGLAVEREGLQAAIIMLAPIVPHITHVLWEALGHSNLLDTAWPVVD
                     ESALVRSSVTIVAQVNGKLRAQFEAPIDASAEELERLALAEAGVQKYLEAVIIRKVIT
                     VPNKLVNIVVGP"
BASE COUNT         1681 a         1674 c         1694 g         1474 t
ORIGIN      
        1 gcctgatcct gtgaggtttt tatgcgccga agaataagac gcaataacga aggctctaat
       61 atcgacatca cgccgtttat gaacctgatg atgatattgg tgcccgccct gctatttaat
      121 gcggtattta cgcgcattaa tattattgat ttaaccctgc cccatgtggc gggtatcagc
      181 ggcacggccg aagaattaaa agataaaaaa atagaagtga ttgtgcgcag cgaccgctta
      241 accgtgaatt ttccggcggg tgttgaagtg caaaccctgc ccttggttaa cggcaaacag
      301 gatagcgaaa ccctctcctt ggtgttgcag agcctgaaga aaaatctggc agggcaggga
      361 gtggataaaa aagacattat gattctctcc gagcccaata cggattatca aaccattgtc
      421 tccattatgg actcggtgcg cagttataaa acggtggtgg cggcctcttt agtgagcacc
      481 gcattattcc ctgaaattgc tttaggcgat gcgccaggtt ccgtggaaat acccgcgcct
      541 gcagtaatac ccggaggtgc gccgtgaaaa ccagcatgcg cgccaaacgc aaataccgca
      601 aagaaaaacg caaacgccct ttggtgatat taaacctggt atccctggtg gatatgttca
      661 ccattttgtt gtgtttttta ttggtaaacg ccccggatgg ggaagtgtta gaaaacgaat
      721 acaatgttaa tttgcccgag tcagtatccg aagttaaacc cagtgggcac ctaattatta
      781 ccgtgactgg ggaggatatt aaagtggccg ggcaatcggt gatgaccgtg gcgcaagcca
      841 ttggcgatgg ccaagtgata ttgcctgccc tgcaacaagt gctgggcgcc gaagcgacta
      901 aaacccctta tttaaccgaa gaagccaaag aaaaaggccg ctttattact gtgatggggg
      961 attcggcagt ctcctacgca ctgttgaaaa aaattatggc cacctgcgcg caaagtgaat
     1021 accgcaatat ttccttagcc attaatcaaa ttgacgcggc ccctgccgca gcgggaggca
     1081 cctgatgagt gccttagtga gcccaaattt attattgccc tgggccaata ttccgcagga
     1141 aaaccgccgc cttaattttg ccctgggctt aggggcgctg gtatttttgg tgttttttgc
     1201 catcgttaaa tgggttgaat taccgcctcc ggcaaaacct gctcctgtgg ccaccacctt
     1261 aatgattgtg gatcaaccgg tggtattgcc gcctccaccc atacctgtgg agccgccacc
     1321 gccagagccg gaaaaggttg agcaaaaacc ggagccaaaa cccgaaccca aaccggaacc
     1381 taagcccgag ccaaaaccgg aacccaagcc ggttaaaacc gagccggtaa aaacacctgt
     1441 aaaagaacct acggctaata acgccgaggt gaagctggat aatgcgcgca aacaagcggc
     1501 ctcagcgggc gtcatgcagg cgcagaacga actggcggcc atgcgcgata gtttaaatat
     1561 cagcgacgta gccaaaccca ccgacgcaac ccccagcgat aataacgcgg ctaacactgc
     1621 cgctacggtg gaccgggcgg ttattagctc cggcgccctg gccaaaagtg gcggcattag
     1681 cgtggccgcc ttaagtaaag acaccggcgg cggtggcagc ctgggcgggc gcaaaggcac
     1741 ggcggtggaa aataaattaa aagatgccga ccaagccgct gctgccaaac aagcggcggc
     1801 cagcgcaggc ggcggcaagg gcagccgcac cgatgaagaa ttaaaacgca ttttagacgc
     1861 agccaaagcg cgcattaccg ccgtgtatta ccgcgcctta gacgacgacc cgacgctgca
     1921 agggcgcatg gtatttaaac tcaccatcga acccagcggc gctgtatcca atgtgctggt
     1981 ggtgtccagt gcgcttaaaa acgatgattt acagcgcaaa attactgcac tgttgcgggg
     2041 tttaaatttc ggtgcgcggg atgtggcggt gtatacacaa acggtgccga ttgatttatt
     2101 cccgtcctga tttttttgtt gtgttgttgg gccgctttga gcggcctttt ttatggagta
     2161 aatctgatcc gaccatttca tgcaagttgt accgcagtag acaggcgtgc ctcataaggc
     2221 tataaaccaa caaatggttt atgtgaacca cttattggtt tatagttcgg ccccgttgtg
     2281 ttttagtaaa ggcctcccct cacgagattg cgctatgttt gtactcaaag gtgttttgcg
     2341 agcggaaagc caggagaaaa ttttgattta tctgttgctg cgggaaacgg gctacgctaa
     2401 aaacatcgcc gaattctatg gcgcacccac caatccaatc caaaaacaat tgctgcgcct
     2461 ggaggaggat ggagttgttg ttggcgccag cctcggtaaa cttaagcaat ataaattaaa
     2521 cccgcgttat ccatttatag agccgctaaa gtcattgctt aaaaaggctt tagaaattta
     2581 ccctcctgaa gtgagggttg agggcttagt tcagcgtaca agaccgcgca aaagtggcaa
     2641 gccattgcag agcctgcgca agtgagcagg ggagtttcca gcattaataa cctgcggagg
     2701 tcaaaatgga ttttagccat acacctatta tcgaactggc caggattatt gccgagcatc
     2761 tgcaagacca ggggattgag gtggttttgg tgggcgggtt ggcggtagaa atctatacgg
     2821 aaaacattta tctcaccaaa gatatcgaca tggtgaatac caactatgcc tctagccgcc
     2881 accttaatac cgctatggct cagttaggat ttgccaaaaa aggccgcatt tatgtaaatc
     2941 ctactactga tattactgtg gaatttccaa ctgggccttt atccgtgggg gatgaattaa
     3001 ttacacgggt gactgtcgcc cagctggcca cagggcaaat tcccattttg caggtttctg
     3061 atattgtaaa agaccgcctc gctgcttact ttcactggcg tgatagacaa tccttagtgc
     3121 aagcgctggc aattttagta aaacatccat taaaaattac cagcattaaa gctttttgca
     3181 atcaggagtg tgctgagtct ttttatccag cacttaaaac cctgcttaac aaagcccgca
     3241 agcagaagtt ggctcaaatg gaagagttgg agttaatggt tgccgattat tttattgacg
     3301 agctctaacg caatagaggt agcgttcgga caaaaaaacg ggtgacaaag agttcttatc
     3361 ggatcagttg tatttcaggg caagttctgg cgggttcaag gtgatgcctt ttataaagta
     3421 aatggaggca ggcctcttca ataaggcgcg ctgattactg ccccgccgcc aatctatttc
     3481 gcaaagtggc ttatattcgt taaatcctcg tctgggctga agagaggcaa cttagcgtgg
     3541 caccggtgaa gaatgcccca gtgttgcctt tgaccccgag atccaccatg aggctttcat
     3601 ggttgctcta tgccgagcgc taaacccttg ggctttaccc cccatccccc tataatcccg
     3661 gctttcgtcc cttttgattg cgcctaagtg aaagccatga ctgccatatc cagcgagtac
     3721 accccccaca ccatcgaacc tgccgtgcag gacttttggc gcgatgccaa agcctttgaa
     3781 gttaccgaag atgccagccg ggaaaaattc tactgcctgt ctatgttccc gtacccttca
     3841 ggccgcttgc acatgggcca tgtgcgcaac tacaccatct ccgatgtaat cgcccgctac
     3901 cagcgcatgc tgggcaaaaa tgtgctgcac cccatgggct gggatgcctt tggcctgccg
     3961 gcggaaaacg ccgctatcaa aaataaaacc gccccagccg cctggaccta ttccaacacc
     4021 gcctatatga aaaaacagct cactgagttg ggctttggtt ttgactggtc gcgggaaatt
     4081 actacctgca aaagtgatta ttacaaatgg gagcagtggt ttttcacccg cttgtatgaa
     4141 aagggcttgg tgtacaaaaa aatggccagc gttaactggg acccggtaga tcaaaccgtg
     4201 ctcgccaatg aacaggtagt ggacggtaaa ggctggcgct ccggcgcacc ggtggagcgc
     4261 aaagaaatcc cgcagtggtt tatccgcatt accgattacg ccgaagaatt attggccgat
     4321 ttagacaaat tgccccactg gcctgagcaa gtaaaaacca tgcagcgcaa ttggattggc
     4381 aaaagccgcg gcctggaaat gtattttaaa ttggaaaaac ccctcggcgc ccatttgggg
     4441 tttgatgtct acaccacccg cccagacacc cttatgggcg tcacctatgt aagcctcgcc
     4501 gcccagcacc ccatcgccct ggccctggcg caaacccaca gcgagttggc ggcgtttatt
     4561 gacagctgca aagtgcaatc cctggctgaa gcggatatgg ccaatatgga aaaacgcggt
     4621 atggataccg gccttaaagc cctgcacccg attaccggcg agccggtggc cctgtgggtg
     4681 gccaactatg tattgatgga ttacggcacc ggcgcggtaa tggccgtgcc cgcccacgac
     4741 cagcgggatt ttgaattcgc gcagaaatac aacctgccca ttaaccaagt tatcgcgccc
     4801 ttggatggca gcgccatcga tttaagccag ggcgcttaca cagaaaaagg caagctaatt
     4861 aattccggcg aatttaccgg tttagatttc gccgccgcct ttgacagtat cgccgccacc
     4921 ctcgaagccc agggccgggg ccgcatcacc accaattacc gcctgcgcga ttggggtgta
     4981 tcccgccagc gctactgggg cgcgccgatc cctatgttta acctgcccag cggcggcgaa
     5041 atccccgtgc ccttggatcg cctgcccgta ttgctgccgg aagacgtggt aatggacggc
     5101 attcagtccc ccattaaggc ggaccccaac tggcgccagg cggaatttaa tggccaattg
     5161 gtggagcacg aaaccgacac cttcgacacc tttatggagt ccagctggta ctacgcccgc
     5221 tacacctgcc ccaattttaa agacggcatg ttggacccag ccgccgctaa ttattggctg
     5281 ccggtggatc agtacgtggg cggtattgaa cacgccattt tgcatctttt atatgcgcgg
     5341 tttttccata aattgatgcg tgatgaaggc ctggttaaat gcgacgaacc cttcgagcgc
     5401 ttgctctgcc agggcatggt aaacgctgag acgttcttta aaaaagacag cgaagggcgc
     5461 attacttggg ttgcgccgag caaggtgaaa atagataaag acgaaaaagg ccgtttaatt
     5521 caagccttcc atatggaaac cggtgacccg ttggaatttg gcggtgtggt gaaaatgtcc
     5581 aaatccaaag agaacggtgt ggacccgcaa gaagtcatta atagccacgg cgccgacacc
     5641 atccgtttat tcagcatgtt cgccgcgccc ccagagcaga gcctggagtg gacggattcc
     5701 ggcgtaatag gcgccgctaa ctttttgaaa aaactctggc gcgccgtaca ccaccattta
     5761 gccgctggaa cccctggcac cttggatgca agcagcctta atcaaaccca aaaagacctg
     5821 cgccgcaaaa cccacgaaac cattaaaaaa gtcagcgacg attttggccg ccgccaggta
     5881 tttaacaccg ccatcgccgc cattatggaa ttgctcaacg aactcggccg ccacgccgac
     5941 cgcgccacac cacaaggcct agccgtggag cgcgaaggcc tgcaagcggc aataattatg
     6001 ctcgccccca tagtgccgca cattacccat gtactttggg aagccttagg ccacagcaat
     6061 ttattggaca cggcttggcc tgtggtggat gaaagcgcat tggtgcgtag cagcgtgacc
     6121 atagtggccc aggtaaacgg caaattgcgc gcgcagtttg aagcgccgat tgatgcctcg
     6181 gcagaagagt tagagcgctt ggccctggcg gaagcggggg tgcagaagta tttggaagcg
     6241 gtgattattc gtaaggtgat tacggtgccg aataagttgg tgaatattgt ggtggggccg
     6301 taaaaacctg tgccgcagcc ataattcact gaagaagcgg attgccaatg aagaaagtca
     6361 tcattattgc tgggccaaat ggtgcgggga aaaccacctt tgctcgttca tttctgccgc
     6421 aagaggccca gtgtattaaa ttcataaatg ccgatttaat tgctgcaggt ctgtcgccat
     6481 ttgcaccgga agaagcagca attagtgccg ggcgacagac ctg
//
LOCUS       DAOQGS010000194         6521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5815_length_6521_cov_3.870090, whole genome shotgun sequence.
ACCESSION   DAOQGS010000194 DAOQGS010000000
VERSION     DAOQGS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5815_length_6521_cov_3.870090"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKZ52_08790"
     CDS             <1..456
                     /locus_tag="PKZ52_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNG59910.1"
                     /translation="PELLIKASRGVLIQADGFIGDCLAEPFELIALPGGMPGASNLAN
                     CQPLINLLERQRQNGRALAAICAAPAVILGRLGWLKGKKATGFPAFKQELIDQGAQFI
                     DQAVVQDGLLFTSQGPATAMALAVALVRHLQGDAVADEVANGLLLKKMT"
     gene            901..3243
                     /locus_tag="PKZ52_08795"
     CDS             901..3243
                     /locus_tag="PKZ52_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNG59911.1"
                     /translation="MITRILLLVGLAWSLCPFLPLLPFPYPVFYGLAALCLLWSRRSP
                     WAIYSASLLCALGLGLAQLDRIGKQQLPVEQEGALWQGEIQVQKVRFSSAPFPDGHTY
                     AQVQAKIIRQGPSDNFLPQLSGPLISLSWTNPGELRQGQRYSISAKLQRLSSQLNPAG
                     VDFIAVNLSQGIYLNGQVQKNPAPELLPSPISFIDQLQTQFKSILNQTQAPIYSRFYV
                     AFALGDTSGLSQQDWQILQNTGTVHLMAISGFQIGLMASIGFWLGILVAKPVCLFLNP
                     WPKIQHWPRRLLPSVCSIALATLYTFLADFSLPAERAWVACLLVNGAWLMGLRPSLAQ
                     LWLWCWWLSLAMHPLAPLNTGFWLSYCAVAALLYFSAKKSRSGLFSQAIEAQWIITLA
                     LTLPQLILNIPLSTSGFCANIFIAPWISIVITPLVFILLTASLYTDMSLGFWLLDQAY
                     FIAWWSLEKISQLPATLWWPEHALTLLDIVLAALGSLLLIAPRALGYAFLGGALVVMS
                     LFSPRPKPFAARLDVLDVGQGLAVLIQTPQQAILFDTGASFSARFNAGTHLLTPYLIN
                     QGIHQVNIIISRDNAEHAGGLRGLKQFTWQALITGEPLAEYPQAQLCTADMQGDWDLI
                     HWQVIAPLSGAEDYSRSCALKLTIGSRRIYLFGDISPTQQRALLISESMSDAYILLAP
                     GQGAKNQHLPQLAQRLKPAFTVFSNAARNRYGHPHPKVVEDYQQQGSQVLLTSQLGAM
                     RWEWADENAAPKLTTQWPKRWRYWYPHHSGGNLPQPAPSE"
     gene            3329..4675
                     /locus_tag="PKZ52_08800"
     CDS             3329..4675
                     /locus_tag="PKZ52_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNG59912.1"
                     /translation="MKLPRALFALAASLFLSTEVHAVFPNTDSQGDTLPSLAPMLEKV
                     NPAVVNIATYSTRENNPLLNDPNFRRFFNIPDNAQRQPKQQAAGSGVIIDAGAGTVVT
                     NFHVIKGADEIQVILTDGRNFKAKLLGSDPEADIAVLKIDAKNIAAAPLANSDKLRVG
                     DFVVAIGNPFGLGQTVTTGIVSALGRSGLGIEGYEDFIQTDASINPGNSGGALVNLRG
                     ELIGINTAILAPTGGNVGIGFAIPVNMAKSSIDQILKHGEVKRGQLGVSIQDITPDLQ
                     TAFDLKNGQKGVLITGVAEGSEAEKAGLQPGDLVTSIDGKEVASAAALRNQIGIRSVG
                     DKIKLQLLREGKEKELAVVVGKSQGLEAKSSGMHQLLSGIKFEKNPAGRGLIITKIAP
                     NAPAAQSGLEPGDVIVSANRQAVDSVKQLEQAIGRDSEKILLQINREGGSFFVVIR"
     gene            4782..5309
                     /locus_tag="PKZ52_08805"
     CDS             4782..5309
                     /locus_tag="PKZ52_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59913.1"
                     /translation="MRVLSLTLLSLAAAAALWAECSQCQSASLPPPTPAALTTISQDN
                     LALSGVWVSNCYGVEQTLFSQDTYTFSATGSFSLEVNRYADRQCQAYKGGSNFYGSYQ
                     VGEQVDTMGGEKARKVRMSIDNADWPEGVASQVDWLLQLEQDTLRFGAFNPPFNWINN
                     NVVFQKLKGQGPMVF"
     gene            complement(5506..>6521)
                     /locus_tag="PKZ52_08810"
     CDS             complement(5506..>6521)
                     /locus_tag="PKZ52_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNG59914.1"
                     /translation="LWQRHLNQQGQPQLLVSALGAASASADPIYKHLEKAQAQRESLE
                     QARLIYVACTRAKRHLFLLAEVKPGSEAGQNQAPSKTSILGSLWPGVETLWPVAEVAD
                     PPAEQEAAETPSLKLAPLHRLPLDWAAPPWPRETLLQAYVPPFDYKNQAQSPFAPGCD
                     LPRAIGTLVHQALSAMAEQGMSRWQQHWPGSNPAWPAQLLRLGLPRHLVPAAIKGAQE
                     LIAIALADPFLHTRLNQPERCQAEQTWWQLAEDGESLQQVRPDLLWHSEEGAVWLIDY
                     KTARPQVGQELEEFIQAEKTSYHKTLQQYAQALRASGLQRLKVGLYFVALGRWCEYDD
                     LSG"
BASE COUNT         1447 a         1820 c         1702 g         1552 t
ORIGIN      
        1 cctgaacttc taatcaaggc atctcggggc gtattgattc aggccgatgg ttttattggc
       61 gattgtttag ccgagccgtt tgagctaatc gccctgcccg gcggtatgcc cggcgccagt
      121 aatctcgcca attgccagcc gttaattaac ttgcttgaga gacagcgcca aaacggccgc
      181 gccctggcgg ctatttgcgc tgcgcctgcg gtgatactgg ggcgcttggg ctggcttaag
      241 gggaaaaaag ccacaggctt tcccgctttt aaacaggagc tgatcgacca gggcgcccag
      301 tttattgatc aagcagtggt gcaagacggc ttgttgttta ccagccaggg gccggcaacc
      361 gccatggcct tggccgttgc gttagttcgt catttgcaag gggatgcagt ggccgatgag
      421 gtggccaatg gattattact taagaaaatg acctaaagca ggttctaaaa attggtctta
      481 gaaaatgggg ttaaacgggt acgtccctgt acatcgccac ctttaataac gtctggcacg
      541 actgctcgca cactcgtagc gcccacccca ttcatgcacc aagagcacat tgtttttcca
      601 taagggctaa gggttatgtg cctcgtttta ggcatccgtt tttagccatc ccgaacatta
      661 atcctgtttt atcccttctt aatccttgct cacatccctc gctggcaccg caggggcaag
      721 actaaggggc ggcgggggct ttgtctgtac gtaaatttac gtagagcacg gcatttctcg
      781 ctgtaaagat caatatttgt gactcctgtc accggcctac ccatctattg aacccctcac
      841 tgaggcttga gacaggcagt taagggcgtt aatctggcct ttttgccaag gactttaagg
      901 atgattacgc ggattctgct gctggtggga ttggcctgga gcctgtgccc gtttctgcct
      961 ctactgccct tcccctaccc ggtgttttac ggcctcgcgg ccctgtgttt gctgtggagc
     1021 aggcgcagcc cctgggcgat ttatagcgcg agcctgttat gcgccctggg cttaggccta
     1081 gcccagctcg atcgcatcgg taagcagcaa ttgcccgtgg agcaggaagg cgccctttgg
     1141 caaggggaaa ttcaggtaca aaaggtgcgc tttagcagcg caccttttcc cgacgggcac
     1201 acttacgccc aggtacaggc caaaattatt cgccaagggc cgagtgataa tttcctgcct
     1261 caattaagtg gcccattaat cagcctcagc tggactaatc ctggcgagtt gcgacagggc
     1321 caacgctata gtatcagcgc taaattacag cgcctaagca gccaattaaa tccggccggg
     1381 gttgatttca tcgccgttaa tttatcccaa ggcatttact taaatggcca ggtgcaaaaa
     1441 aaccctgcgc cagaactatt gccctcaccc atcagtttta tcgatcaatt acaaactcaa
     1501 tttaaatcca tcctcaacca aacccaagcg ccgatttaca gccgctttta tgtggccttt
     1561 gccctggggg ataccagcgg tttaagccag caggattggc aaattctgca aaacaccggc
     1621 acggtgcatt taatggctat ttccggtttt caaattggct tgatggcgag cattggtttt
     1681 tggctgggaa ttttagtggc taaacccgtc tgcctgtttt taaatccatg gccgaagatt
     1741 caacactggc ccaggcgttt gttgccctcc gtttgctcta tcgctcttgc caccctatac
     1801 acatttttag cggatttttc gctcccggcg gagcgagctt gggtggcctg cctgctggtc
     1861 aatggcgcct ggcttatggg cctgcggccg agcctggccc aattgtggtt gtggtgttgg
     1921 tggctgtccc tcgctatgca cccgctggcg ccattaaata ctggcttttg gctgtcttat
     1981 tgcgcggtgg ccgccctgct gtatttcagc gccaaaaaat cccgcagcgg actatttagc
     2041 caagccatcg aagcccagtg gatcattacc ctcgccctca ccctgcccca attaatcctt
     2101 aacattccac tttccaccag cggcttttgc gcgaatattt tcattgcccc ctggatttcc
     2161 atagtcatta caccgctggt atttatttta ttaacggcga gtttatatac cgatatgagc
     2221 ctgggttttt ggctgctgga tcaggcctat tttatcgctt ggtggagctt agaaaaaatt
     2281 agtcaactgc ccgccaccct ttggtggccc gagcacgcct taaccctgct ggatattgtc
     2341 ctggccgccc tgggcagcct gttattaatc gcgccgcggg cattagggta tgctttttta
     2401 ggcggcgcct tagtggtaat gagtcttttc tcaccccggc ctaaaccctt tgcagcgcgc
     2461 cttgatgtat tggatgtggg ccagggttta gccgtattaa ttcaaacacc ccagcaggca
     2521 attttatttg acacaggggc gagtttctcc gcccgtttta atgccggcac ccacctatta
     2581 acaccctatt taattaacca gggtattcac caggtaaaca taattatcag ccgggataat
     2641 gccgaacacg ccgggggttt gcgcgggtta aaacaattta cctggcaagc attaataacc
     2701 ggcgagccct tagccgaata tcctcaagct cagttgtgta ccgctgacat gcagggcgac
     2761 tgggatttaa ttcactggca ggtcatcgcc cctttatccg gcgcggaaga ttattcgcgc
     2821 agctgcgcac ttaaactcac tattggcagc cgccgtattt atttattcgg cgatatatcc
     2881 cccacacaac agcgcgccct attaattagc gaatcaatga gtgacgctta tatcctgctc
     2941 gcccccggcc agggcgctaa aaaccagcac ctgccacaac tggcccaacg cctaaaaccc
     3001 gccttcacag tgttttctaa cgccgcccgc aaccgctacg ggcaccccca ccccaaggtg
     3061 gtggaggact atcagcagca gggcagccag gtgttattaa cctcacaact gggcgctatg
     3121 cgctgggagt gggccgatga aaatgccgcc cccaagctca ccacccagtg gcccaagcgt
     3181 tggcgttatt ggtatcccca ccacagcggc gggaacttgc cgcagcctgc gccctcagaa
     3241 taagcccgca gggacgagca ggtaggaact tgaatttacg caatggagcc taattatgtt
     3301 tttgctttta acccgcagag gaatgtccat gaaattacca cgcgccttat tcgctttggc
     3361 tgccagttta ttcttgagca cagaggttca cgccgtattt cccaatacgg atagccaagg
     3421 cgacacactg ccaagccttg cgcccatgtt ggaaaaagtg aacccggcgg tggtgaatat
     3481 cgcaacctac agcacgcggg aaaacaaccc actgctcaac gatcctaatt tccggcgttt
     3541 ttttaatatt cccgacaacg cccagcgcca acccaaacaa caagcggcgg gctccggggt
     3601 gattattgat gcaggtgccg gtacggtggt gactaatttc cacgtcatca aaggcgccga
     3661 tgaaatccag gttattctta ccgacgggcg caactttaaa gcaaaattgc tcggctctga
     3721 tccagaggcc gatattgccg tattgaaaat agacgctaaa aatatcgccg ccgctcccct
     3781 tgccaatagc gataaattgc gggtggggga ttttgtagtg gccatcggca acccgtttgg
     3841 tttgggccaa accgttacca ccggaattgt cagcgcctta ggccgcagcg gcttgggtat
     3901 tgaaggttat gaggatttta ttcaaaccga tgcctccatc aacccaggta attccggcgg
     3961 tgcactggtg aatttacgcg gcgaattaat cggcattaat acagccattc tggcccccac
     4021 tggcggtaac gtggggattg gttttgccat tcccgtcaat atggctaaat ccagtattga
     4081 tcaaattctt aaacacggcg aagtaaaacg cggccaattg ggcgtgagca ttcaggatat
     4141 tacgccggat ttacaaaccg cgtttgattt aaaaaatggc caaaaaggtg tgttaattac
     4201 cggcgtggcc gagggctctg aggcggaaaa agccggcctg caaccggggg atttagtcac
     4261 ctccatcgac ggtaaagaag tggcctccgc agctgccctg cgcaatcaaa ttggaattcg
     4321 ctcggtgggc gataaaatta aattgcaatt attgcgcgaa ggcaaagaaa aagaacttgc
     4381 tgtggtggtg ggcaaatccc agggcctaga ggctaaaagt tccggcatgc accaattgtt
     4441 aagcgggatt aagttcgaga aaaaccccgc tgggcgcggc ttgatcatca ccaaaattgc
     4501 ccctaatgca cccgctgctc aatccggctt agagccaggc gacgtaattg tcagcgccaa
     4561 ccggcaggcg gtggactcag ttaaacagtt ggaacaggcc ataggccgcg acagcgagaa
     4621 aattttgttg cagattaacc gtgagggcgg tagcttcttc gttgtgatca ggtaagctac
     4681 ctgcgccaat ctgaacggta tttgcccgtt ttgtggcaaa taccgttttt ttatgggcgc
     4741 aacctgctag ccttaactca actcctatac cagacagaac tatgcgcgta ctctcactca
     4801 cactgctctc cttagccgcc gctgctgcgc tatgggctga gtgttcccag tgtcagtccg
     4861 caagcctccc ccctcctacc cccgccgcac tcaccaccat cagccaggac aatttggccc
     4921 tgagcggtgt ttgggtaagc aattgttatg gggtggagca gaccctattc agccaggata
     4981 cctatacctt ctctgccacc ggcagcttca gcctggaggt caaccgctac gccgaccgcc
     5041 agtgccaggc ctataaaggc ggcagtaact tttacggctc ctaccaggtt ggcgagcagg
     5101 tggacaccat gggcggggag aaagcgcgca aggtgcgtat gagcatcgac aacgccgact
     5161 ggccggaggg agtagccagc caagtggatt ggctgttgca gctggagcaa gacaccctcc
     5221 gcttcggcgc ctttaaccca ccctttaact ggattaacaa caatgtggtg ttccagaagt
     5281 taaagggcca ggggcctatg gttttttaac atccttgctc aaactcataa gttagataac
     5341 tatccaagat gcagcgcaag caaaatccgg gttttcgggt gcaaaggcct atctacctac
     5401 ccgcatttgc aaaggcctag ctcaacatta cccatattcc gttgataccc ggattccgct
     5461 cgtgctacat cccggctacc gatgcaaaag ccaccgaaca aaccgctagc cgctgagatc
     5521 gtcgtactca caccagcgcc ccagggccac aaaatacaaa ccaactttta acctctgaag
     5581 accactggcc ctaagcgctt gggcgtattg ctgtagggtt ttgtggtaac tggttttttc
     5641 cgcctgaata aactcctcca gctcctggcc cacttggggc cgagcggttt tgtagtcgat
     5701 caaccacacc gccccctcct cgctatgcca gagcaagtcc ggccgcacct gctgcaggct
     5761 ctcgccatcc tcagccagtt gccaccaggt ctgctccgcc tggcagcgct ccggttgatt
     5821 cagccgcgtg tgcaagaaag ggtcagccaa ggcaatggct atcagctctt gggcgccctt
     5881 tatcgccgct ggaaccagat ggcggggcaa gcccaggcgc agcagctgag cgggccaggc
     5941 ggggttgctg ccgggccagt gctgttgcca gcggctcatg ccctgctcag ccatagcact
     6001 tagggcttgg tgcactaggg tgcctatggc tcggggcagg tcacagccgg gcgcaaaggg
     6061 gctttgcgcc tggtttttat aatcgaaggg cggtacatag gcctgtaata gggtttccct
     6121 cggccatggc ggcgcagccc agtcaagggg caatcgatgt aggggcgcaa gctttaggct
     6181 cggtgtctcg gccgcttcct gctcggcggg cgggtcagca acctcagcca ccggccagag
     6241 ggtttcaaca cctggccaca ggctaccgag gattgaagtt ttggagggag cctggttctg
     6301 gccggcttca ctaccgggtt ttacttccgc caacaaaaat aaatgccgtt tggcacgggt
     6361 gcaggccacg taaataaggc gggcctgctc aagagactcc cgctgggcct gggctttttc
     6421 taagtgtttg tagatggggt cggcgctggc actggcagca cccagggcgc ttaccaacag
     6481 ctgcggctgc ccttgctggt taaggtgccg ctgccacagc c
//
LOCUS       DAOQGS010000195         6514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5824_length_6514_cov_4.405636, whole genome shotgun sequence.
ACCESSION   DAOQGS010000195 DAOQGS010000000
VERSION     DAOQGS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5824_length_6514_cov_4.405636"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1129
                     /locus_tag="PKZ52_08815"
     CDS             227..1129
                     /locus_tag="PKZ52_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG59915.1"
                     /translation="MIERHHLALLRNLAHTGSLAAAAEKLHLTQSALTHSIRKLEQIT
                     GVTLWEKQGRKLQLTAAGRYLLATGEEVLPRLEAADQQLKALASGQLGQLRIGMECHP
                     CYAWLLKQLKVFLPRWPGVELDVIQRFRFNGREALEQHAIDMLITSDPIQATHLSNIP
                     VFNYALQLIAAQHHPLTQHQWITPQDLADQTLFTFPVDTQRLDVFTQFLSPAGIKPRN
                     HKTLEALEIMLLLVATGRGVCTLPDWLASQLQSEYTVTTIGLGPQGVQKTLYLVVRQG
                     DTAISYMEDFIRISQGGAARDKAK"
     gene            1576..1956
                     /gene="ndhC"
                     /locus_tag="PKZ52_08820"
     CDS             1576..1956
                     /gene="ndhC"
                     /locus_tag="PKZ52_08820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNG59916.1"
                     /translation="MAAYLGGVVFLLAFMLGIPWLLGGRATGRAKNDPFESGVVSQGG
                     ARLRLSAKFYLVAMFFVIFDVEALYLFAWSVSVRESGWVGFAEAVTFIVILLAGLVYL
                     VRIGALDWAPEQLRRRKLRQKESK"
     gene            1966..2640
                     /locus_tag="PKZ52_08825"
     CDS             1966..2640
                     /locus_tag="PKZ52_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNG59917.1"
                     /translation="MKYTLTRPNLDKPGQQYPLQDQQTVSDPLEDQVHKSVFMGKLED
                     MLHGVVNWGRSNSLWPYNFGLSCCYVEMATAFTAPHDLARFGAEVIRASPRQADLMVV
                     AGTCFIKMAPVVQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIP
                     GCPPRPEAFLQALTLLQDSIGKERRPLSWVVGEQGVYRADMQAERDRKQAERIAVTEL
                     RSPDQI"
     gene            2664..4442
                     /gene="nuoC"
                     /locus_tag="PKZ52_08830"
     CDS             2664..4442
                     /gene="nuoC"
                     /locus_tag="PKZ52_08830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HNG59918.1"
                     /translation="MTDTVTQTPLAPGAQHAVVLELYSRFGSEAFTFQSTLDNTPTLW
                     VPRDLLVDVLRFLRGLPQPYVMLYDLSATDERLRQHKYGLPIADFTVFYHLISIERNS
                     DVRIKVALGSSDLNLPSVVGIWPNANWYEREVWDLFGIVFEGHPHLSRILLPNYWEGH
                     PLRKDYPARATEFDPFRLNAAKQDLEQEALLFKPEEWGMQKGTENEDFMFLNLGPNHP
                     SAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMAERQTWHSFIPYTDRIDYLGGVMN
                     NLPYVLAVEKLAGIEVPERVKTIRVMLAEFFRITSHLLYLGTYIQDVGGMSPVFFMFT
                     DRMKAYDVIEAITGFRMHPAWYRIGGVAHDLPKGWDKLVREFIEWLPPRLREYEKAAV
                     KNSIVRTRSVGVAQYNKDEALAWGVTGPGLRATGVNFDLRKARPYSGYENYEFDVPLA
                     NNGDAYDRCLLRLEEMHQSVRIIKQCLENMPAGAYKADHPLTCPPPKERTLQHIETLI
                     THFLSVSWGPIMPAGESSQMIEATKGINSYYLTSDNSTMSYRTRIRTPSFAHLQQIPS
                     VIRGSMIPDLIAYLGSIDFVMADVDR"
     gene            4429..4968
                     /gene="nuoE"
                     /locus_tag="PKZ52_08835"
     CDS             4429..4968
                     /gene="nuoE"
                     /locus_tag="PKZ52_08835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNG59919.1"
                     /translation="MSTANSSNSSTPYSALIATENGAPFALTEAERHEIEHAIHHYED
                     PRAASIDALKIVQKYRRWVPDGAIALIAELLSISAADVEGVATFYNRIYRQPVGRHVI
                     LVCDSIGCFLTGFDEVYAALQSKLAIQPGQTTKDDRFTLLPVCCLGACDRGPVLMIDD
                     DTHFNVSPSNLNQVLEQYA"
     gene            4965..6272
                     /gene="nuoF"
                     /locus_tag="PKZ52_08840"
     CDS             4965..6272
                     /gene="nuoF"
                     /locus_tag="PKZ52_08840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNG59920.1"
                     /translation="MSSNVNLGLTNEGPETHPLTWRYTQAGKAVLDIQDYEALEGYSA
                     LKKAYSMPQLDVQAEVKASNLRGRGGAGFPTGVKWSLIPMGPDAGQKYLVCNADEMEP
                     GTFKDRMLMEELPHQLVEAMAVAAYAIGASKGFIFLRGEYILAAERLNAAIDQAKAAG
                     YLGENILGTGFNFELFVHTGAGRYICGEETALINSLEGRRANPRTKPPFPQAAGAWGK
                     PTIVNNVETLMNIPPIVLRGADWFKGLSQGKSPDAGTKMYGISGRAKITGLWELPIGT
                     SCRELLYTHGGGMADGLELACWLPGGASTDFLMPEHLDLPMDYDTIMKAGSRMGTALI
                     MVVDNKQNIVSVVRNLEQFFARESCGWCTPCRDGLPWTVKILQAIEKGEGEAGDIETL
                     QQLTRFLGPGKTFCAHAPGAMEPLQSALKFFPDAFKQGIRAEA"
BASE COUNT         1460 a         1938 c         1702 g         1414 t
ORIGIN      
        1 agtaggattc attggcccat tttaattccc gctgtgcacc gatgcgggga aaacccaaat
       61 tatgtgttgt aaccatgtgt attcacctta ataaaatttt tagccgattt tcgttaattt
      121 ggcgcttacc ctgactccac ccaattaatt cagccatgaa aaaactttac ttaggaaaac
      181 acatgattaa aactgatata aattcaaact tacatgagcc aaattcatga ttgagaggca
      241 tcacttagcg ctcctgcgca atctagccca caccggcagc ctcgccgcag cggcggaaaa
      301 actgcactta acacagtccg ccctcaccca cagcattcgc aaactcgaac aaataaccgg
      361 tgtaactctg tgggaaaagc agggccgcaa gctgcaatta actgcggcgg ggcgctattt
      421 attagccacc ggtgaggagg tattgccgcg cttagaagcc gccgatcagc aattaaaagc
      481 cttggcaagc ggccaattgg ggcaattgcg catcggcatg gaatgccacc cctgttatgc
      541 atggctgctc aaacaattaa aagtatttct gccgcgctgg ccgggggtgg agttagatgt
      601 gatccagcgc tttcgcttta atggccgcga ggcccttgag cagcacgcaa ttgacatgtt
      661 aatcaccagc gaccctattc aagctacgca tttaagcaac attccggtat ttaattatgc
      721 actgcaatta attgccgccc agcaccatcc gcttacccag catcaatgga ttaccccgca
      781 ggatttagcc gatcaaacgc tattcacctt tcccgtcgat acccagcggt tagatgtgtt
      841 tactcaattt ctctcgcctg cggggattaa gcctcgcaac cataaaacgc ttgaggccct
      901 ggaaattatg ttgttattgg tggccacggg gcgcggcgtg tgcaccctgc ccgattggct
      961 ggcaagccaa ttacagagcg aatacaccgt tacaaccatt gggctcggcc ctcaaggcgt
     1021 gcaaaaaacc ttatatcttg tagtgcggca gggcgacacg gccatttcat atatggagga
     1081 ctttattcgc atcagccagg gcggcgcggc gcgcgacaag gcaaaataaa acgcctaagg
     1141 cccggcaaac gggtggctaa ggcgcacaca cacggctaat gcaacaggcc tgtaaggttt
     1201 cccacaataa cccgcacaga agaagaatcc gcacagctag caagcctacc caatttggcg
     1261 ccattttttg tgcagccaag gccgctcctg ggcgccaagc ctctgttatc tttaatattc
     1321 tttgttttaa caggctgatt gcacggtttt tgctggccgc tctcgccaat tgccacacag
     1381 gccaggcgac aaaagttccg cgccaaatta gaacgtgttg ttgttacttt tgtgccttgg
     1441 tatacttttc ggctatctag gccctaaaga agtgcgtccg gcatctgtcg gagggccgct
     1501 tttttgactt gaattgtcat tgtcggagct acttgcttgt cgcttacacc tgcacagttt
     1561 catgaatacc tcaccatcgc cgcctactta ggcggcgtgg tgtttctgct ggcctttatg
     1621 ctcggcatcc cctggttact gggcggcaga gccaccggcc gcgccaaaaa cgacccgttt
     1681 gaatccgggg ttgtctccca gggcggcgcc cgtttaaggc tctccgccaa gttctatctg
     1741 gtggctatgt tcttcgtgat cttcgacgtg gaagcccttt atttatttgc ctggtccgtc
     1801 tcggtgcgcg agtccggctg ggtgggcttt gccgaagccg ttacctttat cgtcattctg
     1861 ttagctgggc ttgtgtacct ggtgcgcatc ggcgcgctgg attgggcacc tgagcaattg
     1921 cgccgccgca agctgcgcca aaaagaatcc aaatagagcc tggttatgaa gtacacactt
     1981 acccgcccca acctcgacaa acccggtcag cagtacccgc tgcaggatca acaaaccgtt
     2041 tcagacccgc tggaagatca ggtccataaa agtgttttta tgggcaagct ggaagacatg
     2101 ctgcacgggg tggtgaactg ggggcgctcc aattctctat ggccatacaa cttcggcctc
     2161 tcctgctgct acgtggagat ggctaccgcc ttcaccgccc cccacgacct ggcccgcttc
     2221 ggcgccgagg taatccgcgc ctcgcctcgc caggctgatt taatggtggt ggccggtacc
     2281 tgttttatca agatggcacc tgttgttcag cgcctgtacg agcaaatgct ggaacccaaa
     2341 tgggtgattt ccatgggcgc ctgcgccaac tctggcggca tgtacgacat ctattccgtg
     2401 gtgcaaggcg tggataaatt tttaccggtg gatgtgtaca tccccggctg cccaccccgc
     2461 ccagaggcct ttttacaagc ccttacgctg ctgcaagatt ccatcggcaa agagcgccgc
     2521 ccgctgtcgt gggtggtagg tgaacagggt gtgtaccgcg ccgatatgca ggcggagcgc
     2581 gaccgcaaac aagccgagcg cattgccgtt accgaactgc gcagccccga ccaaatttaa
     2641 cgattccttg gcgacttgat cgaatgactg acaccgtcac ccagacaccg cttgcgcccg
     2701 gcgcccaaca cgccgtggtg cttgagctct acagccgctt tggcagcgaa gccttcacct
     2761 tccagtccac cctggataac accccaactc tatgggtgcc acgggacttg ctggtggatg
     2821 tgctgcgttt cctgcgcggc ctgccgcagc cctatgtgat gctgtacgac ctgtccgcca
     2881 cggacgagcg cctgcgccag cacaaatacg gtctgcccat cgctgacttc acggtgttct
     2941 accacctcat ttccattgag cgcaattctg atgtgcgcat caaggtggcc ttgggcagta
     3001 gcgatctgaa cctgccatcg gttgtaggca tttggccaaa cgccaattgg tacgagcggg
     3061 aagtctggga tctatttggg atcgtttttg aaggccaccc tcacttatcg cgcatcctgc
     3121 tacccaacta ttgggaaggc cacccgctgc gcaaggacta tccagcccgc gccaccgaat
     3181 ttgacccctt ccgcttgaat gccgccaaac aggacttaga gcaggaagcc ctgttattta
     3241 agcccgaaga atggggcatg caaaagggca ccgagaacga ggacttcatg ttcttaaacc
     3301 tcggccccaa ccacccctcc gcccacggtg ccttccgtat cgtgttacag ctggacggtg
     3361 aagaaatcgt cgattgtgtg ccggacatcg gctaccacca tcgcggcgcc gagaaaatgg
     3421 ccgagcgcca aacctggcac agctttatcc cctacaccga ccgcatcgat tacctgggcg
     3481 gtgtaatgaa caacctgccc tatgtattgg cggtggaaaa actcgcgggc attgaagtgc
     3541 ccgagcgggt taaaaccatc cgcgtgatgc tggcggagtt cttccgtatc acctcccacc
     3601 ttttgtacct gggtacttat attcaggacg tggggggcat gtcgccagtg ttctttatgt
     3661 tcaccgaccg catgaaagcc tatgacgtta tcgaagccat cactggcttt cgtatgcacc
     3721 cggcctggta ccgcatcggc ggtgtggccc acgatctgcc caagggctgg gacaaactgg
     3781 tacgggaatt tatcgaatgg ctgccgccgc gcctgagaga atacgaaaag gccgccgtga
     3841 aaaactccat cgtgcgcacc cgctcggtgg gcgttgccca gtacaacaaa gatgaagccc
     3901 tcgcctgggg tgtaacaggc ccaggcctgc gggccactgg ggttaatttt gacctgcgca
     3961 aagcgcgccc ctactccggc tatgagaact acgagttcga tgtgcctctg gcgaacaatg
     4021 gcgacgctta cgaccgctgt ctgctgcgtt tagaagaaat gcaccagagc gtgcgcatca
     4081 tcaagcagtg cttggaaaac atgccagcgg gcgcctataa ggccgaccac ccgctgacct
     4141 gtccgccgcc gaaagagcgc accctgcagc acattgaaac cctgatcaca cactttttat
     4201 ccgtgtcctg ggggccaatc atgcccgccg gtgagagcag ccagatgatt gaggccacca
     4261 agggcatcaa cagctattac ctcacctccg acaacagcac catgagctac cgcacgcgta
     4321 ttcgcacgcc gagctttgcc catttgcaac aaataccttc ggtaatccgc ggctctatga
     4381 tcccggattt aatcgcctac ctaggctcaa ttgactttgt gatggccgat gtcgaccgct
     4441 aactcatcca actccagcac gccctactcc gctctgatcg ccaccgaaaa cggtgcgccc
     4501 tttgcgctga ctgaggctga acgccatgag atcgaacacg ccattcatca ctatgaagac
     4561 ccgcgtgccg cctccatcga tgcgcttaag atcgtgcaga agtaccgccg ctgggtgccc
     4621 gatggcgcca tcgccctgat cgccgagctg ctgagtattt ctgccgccga cgtggaaggt
     4681 gtggccacct tctataaccg tatctaccgg caaccggttg gccgccacgt cattttggtg
     4741 tgcgatagca tcggctgctt cctcaccggc tttgatgaag tctacgccgc cttgcaaagc
     4801 aagctggcga ttcagcccgg ccaaaccact aaagatgatc gctttactct gctgcccgta
     4861 tgctgcttgg gcgcctgcga tcgcggcccg gtgttgatga ttgacgatga cactcacttt
     4921 aatgtcagcc ccagtaacct caatcaggtc ttggagcaat acgcatgagt tcaaatgtaa
     4981 acctcggctt aaccaacgaa ggcccagaaa ctcaccccct cacttggcgt tacacccaag
     5041 cgggcaaagc cgtgttggac atccaagact acgaagcctt agaaggctac agcgccctga
     5101 aaaaagccta ctccatgccc caattggatg tgcaggcgga agtaaaagcc tccaacctgc
     5161 gcggccgggg cggcgcgggc ttccctaccg gcgttaagtg gtccttgatt cccatggggc
     5221 ccgatgcggg gcaaaaatat ctggtctgta acgcggacga gatggagccc ggcaccttta
     5281 aagaccgcat gctgatggaa gaactacccc atcaattggt ggaggccatg gcggtagccg
     5341 cctatgccat aggtgccagc aaaggcttta tcttcctacg cggcgaatac attcttgccg
     5401 ccgagcgctt aaatgccgcc atcgaccagg ccaaagcggc cggttacctg ggcgagaaca
     5461 tcctgggtac aggctttaac ttcgagctgt ttgtacacac cggcgccggc cgttatatct
     5521 gcggtgaaga gaccgcgctg atcaactcct tagagggccg ccgcgccaac ccgcgcacca
     5581 agccgccctt tccgcaggcc gctggcgcct ggggcaagcc caccatcgtg aacaatgtgg
     5641 aaaccctgat gaatatcccg cccatagtgc tgcggggcgc ggactggttt aagggcctat
     5701 cccagggtaa aagcccagat gcgggcacta agatgtacgg tatttccggc cgcgccaaaa
     5761 ttaccggcct gtgggaatta cccatcggca ccagctgccg ggagctgtta tacacccacg
     5821 gcggcggcat ggcggatggc ctggaattag cctgttggct gcccggcggc gcctctaccg
     5881 actttttaat gcccgagcat ttagacctac ccatggacta cgacaccatc atgaaagcgg
     5941 gcagccgtat gggtacagcg ctgatcatgg tcgtcgataa caaacagaac atagtctccg
     6001 tagtgcgcaa cctggagcaa ttttttgccc gcgagtcctg cggctggtgt acaccctgcc
     6061 gagacggcct gccctggacg gtaaaaatcc tccaagccat cgaaaaaggc gaaggcgaag
     6121 ccggggatat agaaactctg cagcagctga cacgcttttt aggccctggc aaaaccttct
     6181 gtgcccacgc ccccggcgcc atggagccac tgcaaagcgc cctgaaattt ttccccgatg
     6241 cctttaaaca gggtatccgc gccgaggcgt aaggcctgcg cagacactta aaaacagctt
     6301 aaaccggccc gccggttggc acagaattga caagttcaaa ccggcgcgcc ggttcaacat
     6361 aggtgaaaac gcaagaatgg ctaccattca tgtagacggc aaacagtacg aggtagacgg
     6421 cagccaaaat cttttggaag cctgtttaaa cctcggcctt gatattccct acttctgctg
     6481 gcacccggcc ctgggctcgg tgggcgcctg ccgc
//
LOCUS       DAOQGS010000196         6470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5896_length_6470_cov_5.382541, whole genome shotgun sequence.
ACCESSION   DAOQGS010000196 DAOQGS010000000
VERSION     DAOQGS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_6470_cov_5.382541"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PKZ52_08845"
     CDS             complement(<1..940)
                     /locus_tag="PKZ52_08845"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG59921.1"
                     /translation="MSSLFNFINSLKSKKRWLTVGLISLFFTAFTFSVQAKDEHGHDH
                     GNEKAESAAHGETEHDEHEEHEEKAAELTDAQLTTAAIETAQAGPADIQETIPLYGTI
                     EANGERIQHINARFPGIIKSVNKKLGDSVKQDEVLASIEGNESLRTYSVAAAFSGVVV
                     ERNANPGEHTDDKSLFVIADLSTVWVEVAVFPRDLAKVKAGQRVRITQPSSNFHADGN
                     IIAIGSLGSANQSLNARVLLENNERHWIPGMFVNAAVSLATTSVKLAIHNEALQEHEG
                     KQVVFVKTEKGFEPRPVKLGRTDGDLSEVLGGLSAGE"
     gene            complement(937..2235)
                     /locus_tag="PKZ52_08850"
     CDS             complement(937..2235)
                     /locus_tag="PKZ52_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNG59922.1"
                     /translation="MFFFRIGFAVASLLLCVSASAQIADSTQAPINKLSLRDAIEATL
                     ARNPQLSSFTFRNEAIAGELQTAALKPARQISAELENVGGSGELKGTQSAELTLALSS
                     VIELGDKREARMGVVTERQRQLATEQEIVGLDLLTEVTRRFIDTAAAQEQVKLQQSTL
                     TLLNDSTQAIKRRVNIGNAPEAELARSQASQVRTAVELRKAESNLQSAKIKLAAMWGD
                     IEPKFSSVNADLMNPGQIYSLKELLAGLEQNPDIQLFSSEARLRDAELRLALSKRKTD
                     IEWSAGVRRLQATQDSALMVGISVPLSSGNRAAGEIATARANQRLTENERDTALLKLR
                     AQLIGLHQEREAALFEVNSLRNEVIPQLRKAINVTRAAFDKGRYGYQELSSAQHELLE
                     AEAALIEAAANAHQLRAEIERLSGSATTSRLSSQIFEVKP"
     gene            complement(2314..2694)
                     /locus_tag="PKZ52_08855"
     CDS             complement(2314..2694)
                     /locus_tag="PKZ52_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59923.1"
                     /translation="MNILHRHKAFVYAILLLWFFASASGIHGHFCFDGKEPPVSVHFD
                     ILSGHDEHHFDGHKDLDSKPGQLDALKVSSFSFLFLITALLLVIVWPLVQNQKYHLFN
                     IPTSWLSIAGLRPPLRAPPIFSRS"
     gene            complement(2737..2946)
                     /locus_tag="PKZ52_08860"
     CDS             complement(2737..2946)
                     /locus_tag="PKZ52_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59924.1"
                     /translation="MSVVKNPISRFTYAVPTTNYGQFHPNFATAMSIICLTIVFLTTD
                     ALIDENKDVSVAEKVIVFLNENSYY"
     gene            3300..3818
                     /locus_tag="PKZ52_08865"
     CDS             3300..3818
                     /locus_tag="PKZ52_08865"
                     /inference="COORDINATES: protein motif:HMM:NF023108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HNG59925.1"
                     /translation="MIPVNGEITIRQRQGRFGLFNTAILECSIGTFAVRDRSLEQFNE
                     GVYTGVFILKHIRPDSYMAGGRIVVQIIAELDQIILSEDQYEITHDYELEQDPIIQEQ
                     NHRPNVQSIELPAGNSDSDDVAELFGELWPLGAVVKLDSAAERFVLRRQKDYLGQMGY
                     TFDQGSQCWVRR"
     gene            3825..4943
                     /locus_tag="PKZ52_08870"
     CDS             3825..4943
                     /locus_tag="PKZ52_08870"
                     /inference="COORDINATES: protein motif:HMM:NF039653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HNG59926.1"
                     /translation="MALIFQRLARNFIKNGYFPTDELSLTRILSHIAPAEEHGLIRLL
                     DPCCGEGTALAELAHHLKGLGATVYSAGIEYDKQRAYHAKTLLDTTIHADFEHCVVTK
                     EQFQVLWLNPPYGDRLATHLTEKTSNGRDRLEKVFLKNAISSLSFGGLLIYIIPHYVL
                     DKDLSRQLVRYIKDIEVHRLPEAQFKQIVVTGYRKRVDQVSSGDSEALSLLLAVGEDR
                     LAADELCDSKIPTRRVPICSADILKRKLEFKTCIPDKDQVAEVVKKNPCLWADFQQLF
                     CRSGVDIRRPCRKLSDWHLSLTLAAGLVSGIVTSPGGRSLLVKGGTYKTKDRVVTQDI
                     DDKGQVTEVVTATDRFVPIIKAIDVTPGDRFGSIITIR"
     gene            5048..5734
                     /locus_tag="PKZ52_08875"
     CDS             5048..5734
                     /locus_tag="PKZ52_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59927.1"
                     /translation="MRLLEIQINHISMQLYCDAFLLDANQKLMFMSFWGNETVSLEFF
                     ARITIPNHEFGIRDIGFVEEENMPLQKNICVGDPKTLKKFVTKTTYSTIVGTQCHVFI
                     FDERLLKPDRMNMQSIIYREPDESDPSYWDRVWREVKQTSRIGLLDDWKDVLLEWCIQ
                     NNWLTELQGFYLQGHQLLLIEKDFNQAVQNGLTEGALTIPPTLYTAPHKAVPETVKEA
                     QSSGQLALLL"
     gene            5982..>6470
                     /locus_tag="PKZ52_08880"
     CDS             5982..>6470
                     /locus_tag="PKZ52_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNG59928.1"
                     /translation="MESEFGDAVTEPDANEGEVLSLRDFIGEFGSGLLAAVEQNNPPV
                     YTGAVINPKRQHILNALARKPFPAQAEAVYALSALLFDQNEKAAVLNAEMGTGKTMMS
                     ISMAAIALQEGLKRILVISPPHLVYKWRREIQETIKGARVWVLNGPDTLMRLLKLKSL
                     LVT"
BASE COUNT         1699 a         1438 c         1606 g         1727 t
ORIGIN      
        1 tttcgcctgc agagaggccg cccaatactt cactgagatc accatcggtg cggcccagtt
       61 taactgggcg tggttcaaaa cctttttcgg ttttgacaaa taccacttgc ttgccttcat
      121 gttcctgcaa ggcttcattg tgaatggcga gctttactga ggtagtcgca aggctcacgg
      181 cggcatttac aaacatgcca ggtatccaat ggcgctcgtt attttcgagc aggactcggg
      241 cattaaggct ttgattagca gagccaagtg aacctatggc gatgatattg ccatcagcat
      301 gaaaattact ggaaggctgg gtaatacgaa cacgctggcc agcttttacc ttggccaggt
      361 ctcgaggaaa gactgcaact tcaacccaaa cagtagaaag atcggctatc acaaacagcg
      421 atttgtcatc cgtatgttca cctggattgg cattgcgttc taccactaca ccgctaaaag
      481 ctgccgcaac agaataggtg cgcaggctct cgttaccttc aatgctggcc aggacttcat
      541 cctgttttac actgtcgccc agttttttat tgacgctttt aatgatgccg ggaaagcgag
      601 cattaatatg ctggatacgc tcgccattag cctcaattgt gccgtaaagc ggaatggttt
      661 cctggatatc ggcaggccct gcctgagccg tttcaatagc agctgtggtt aattgcgcat
      721 ccgttaattc agcggctttt tcttcgtgtt cttcgtgctc atcatgttct gtttctccat
      781 gtgcggcaga ttccgctttt tcattaccat ggtcatggcc atgctcgtct ttagcctgaa
      841 ctgaaaaggt gaatgcggta aaaaatagac taatcaggcc aacggtaagc caacgttttt
      901 ttgacttaag agaatttata aaattgaata aggagctcat ggtttaacct cgaaaatttg
      961 tgatgacagg cgcgaagtgg tggcactacc actcaggcgt tcaatttcag cgcgtaattg
     1021 atgggcattg gcggcggcct ctatcagtgc agcttcagct tccagtaatt catgctgggc
     1081 actgcttaac tcctgatagc catagcgacc tttatcgaaa gcggcacgag taacattaat
     1141 ggctttacgg agctggggaa tcacttcatt gcgtaagctg ttgacttcaa acagggctgc
     1201 ttcgcgttcc tgatgtaatc cgatcagctg cgcgcgtaat ttaagcagag cggtatcgcg
     1261 ttcgttttcg gttaggcgtt gattggctcg tgccgttgca atttcacctg cggcgcgatt
     1321 gccactcgat agcgggacac tgatccctac cattagcgcg gagtcctggg tggcttgtaa
     1381 ccttcgaaca cctgcactcc attcgatatc ggttttgcgc ttgctgagcg caaggcgcaa
     1441 ttccgcatcg cgcaaccggg cctcgcttga aaataattgg atatccggat tttgctctag
     1501 tcctgccaag agctccttaa gcgagtaaat ttgacctggg ttcattaggt cagcatttac
     1561 agaggaaaat ttgggttcga tatcccccca catagctgcc aatttaattt tggcggactg
     1621 caagttcgac tctgctttac gcagctcaac ggcggttcgc acttggctgg cctgtgacct
     1681 ggcaagctct gcttccggtg cattaccgat attgactcgg cgcttaattg cctgtgtaga
     1741 atcattaagg agtgtaagcg tggattgctg taacttaact tgctcctgag ccgctgctgt
     1801 atcgataaag cgtcttgtaa cttccgtgag tagatctagc ccaacaattt cctgttccgt
     1861 agccagctga cgctgccgtt ccgtgacaac acccatacgg gcttcccgtt tatcgcctaa
     1921 ttcaataacg gaggatagag caagtgtcag ctcggcagac tgagtacctt taagctctcc
     1981 actaccgccc acattttcca gttcggcgga tatttgtctt gccggtttta aggcggctgt
     2041 ttgcagttca cccgctatag cttcattgcg gaaagtaaaa ctactgagct gagggttgcg
     2101 tgcaagggtc gcttcaatag catcacgtag cgacaactta ttgataggtg cttgggtaga
     2161 atccgctatt tgggctgatg cggatacgca aagcaacagg ctcgccacgg cgaacccgat
     2221 tcgaaaaaaa aacatggtgt actccagcta aacgcaaata ttgcgcaata catctgagtt
     2281 aattgccaag cgacaatgct cggcagggta aggtcaggag cgggagaaaa taggcggggc
     2341 gcgcagcggt gggcgcaagc ccgcaatgct gagccaggac gtgggaatat tgaataaatg
     2401 atatttttgg ttctgtacaa gcggccaaac gataacaagc agcagtgctg taatgagaaa
     2461 taggaaagag aaactggaca cctttagagc atcaagttgc cctggtttgc tatcaagatc
     2521 cttatgtccg tcaaagtgat gttcatcatg gccactgaga atgtcaaaat gtacggaaac
     2581 tgggggttcc tttccatcaa aacaaaaatg cccatggatt ccgctggcac tggcaaaaaa
     2641 ccacagcaac agaatcgcat atacaaaggc tttatggcga tggagtatat tcaatggagc
     2701 gacctaacag gagcggaacc gcatgcgtat caacttttaa taatagctat tctcattcaa
     2761 aaaaacaatt actttctctg cgactgaaac atccttattt tcatctatca gtgcgtcggt
     2821 ggttaaaaac acgattgtta gacaaataat cgacattgcg gtggcaaaat tagggtgaaa
     2881 ttgaccgtag ttggtggttg gaaccgcgta tgtaaagcgt gaaatcgggt ttttaaccac
     2941 cgacattcat atcacccggg tttttaacca ccgcctgaag cccttagtcg ttaaacaata
     3001 aggcggagcg gtcgtgattg aggtcagaat gtagattctg gattgctctc tgtgggaaat
     3061 cgcctacaac caatatcaag aatgtatcac acggattgta tgaaatatct cgttgtttta
     3121 aacgccagcc tatgagaatc tagctgcgca aaatgtccca gtgaacatat cactgacctg
     3181 caagagctgt gcaaggagtc tgtaaccgca gcatggtcgt tgaggcctga ttcttttttt
     3241 aacccgcgtg ggagttttcc catggcggca aaactctccc gcatttttgg agagttttta
     3301 tgatccctgt caacggtgaa atcaccatta ggcaacgcca aggccgtttt ggcctgttta
     3361 acaccgccat actggaatgt tccatcggga cattcgccgt gcgggataga tccctggagc
     3421 agtttaacga aggggtttac accggtgtgt tcatcctcaa gcatattcgc cctgactcct
     3481 atatggctgg tgggcggata gttgttcaaa tcattgcaga gcttgaccag atcattctca
     3541 gcgaagatca atatgagatc acgcatgatt atgagctaga gcaagacccc attatccaag
     3601 agcaaaatca ccgccccaat gttcaatcta tagagttgcc agcgggtaac tccgacagtg
     3661 acgatgtcgc cgaactattt ggtgaattgt ggccattagg tgcagtggta aaactcgatt
     3721 cagcggcaga gcgctttgtt ctgcggcggc aaaaagatta cctaggtcag atgggatata
     3781 cctttgacca gggtagccaa tgctgggtaa ggaggtaagc gaccatggct cttattttcc
     3841 aacggcttgc ccgtaatttt attaaaaacg gctacttccc aacggatgag ctttctttaa
     3901 ctcgcatcct gtcgcatatc gcaccggcgg aagaacatgg tctcatcagg ctgctagacc
     3961 cttgttgtgg tgagggcaca gcattagctg agttggccca tcacctcaag gggctggggg
     4021 cgaccgttta ctctgcaggt attgaatatg acaagcaacg ggcttatcac gctaaaacct
     4081 tgttagacac aacgatacat gctgattttg agcactgtgt tgtaactaaa gagcagttcc
     4141 aggtgttatg gctgaaccca ccttatggtg atcgattagc cactcacttg actgaaaaaa
     4201 cgtcaaacgg tcgtgatcgg ttagaaaaag tatttttaaa aaatgcaatt tcgagcctgt
     4261 cgtttggtgg attattaatt tatatcattc cgcattatgt tctggataag gatttatccc
     4321 gtcaattggt tcggtacatt aaggatattg aggtacaccg attacccgag gcgcaattta
     4381 aacaaattgt ggtcacaggg tatcgaaagc gtgtggatca ggtttcatcg ggtgattctg
     4441 aagcgctaag cctgttactg gccgtaggcg aggataggtt agccgcagat gaattgtgcg
     4501 attctaaaat tccgacgcga cgcgttccaa tctgtagtgc cgatatatta aaacgcaaat
     4561 tggaatttaa aacgtgtatc cccgataaag atcaggtggc agaggtggtg aaaaaaaatc
     4621 cctgtttatg ggccgatttt caacagctct tctgtcgctc tggtgtggat attaggaggc
     4681 cgtgccgaaa gcttagtgat tggcatctct cgttaacgct agccgctggc ttagtttcag
     4741 ggattgtcac ctcaccagga ggacgatcgt tactggttaa gggcggcacc tataaaacga
     4801 aagatcgagt ggtaacccag gacatcgatg acaaagggca agtgactgaa gtggtaaccg
     4861 caacagatcg ctttgtgccg attattaaag ccatcgatgt cacacccggt gatcgctttg
     4921 gttcaattat tacaattcga tagcgtataa aatcgctgtc acattattaa tcggttgcgt
     4981 tgcaatcatt tcctgcccgg tagagagtcc ctaccgggac tcttttaaat ataaaagaga
     5041 ggaatctatg cgtttattag aaattcaaat taaccatatt tcaatgcagt tatattgcga
     5101 tgcgtttttg ctggatgcca atcaaaagct gatgtttatg agcttttggg gcaatgaaac
     5161 agtatcgcta gaattctttg caaggatcac catcccgaac catgaatttg gcatcaggga
     5221 tattggcttt gtggaggagg agaatatgcc actgcaaaaa aatatctgtg tgggcgatcc
     5281 taaaacatta aaaaaattcg tgactaaaac gacctatagc acaattgttg ggacgcaatg
     5341 ccacgtattt atttttgatg aaaggcttct taagcccgat cgcatgaata tgcaatcaat
     5401 catttatcgc gagcctgacg aatcggatcc cagttactgg gatagggtgt ggagggaggt
     5461 aaaacagacc agccgaatcg gtttgctcga tgactggaag gatgtgctat tggagtggtg
     5521 tatccaaaat aattggttaa cagagttgca gggattttat ctgcaagggc atcagcttct
     5581 gttaatcgag aaggatttta accaagctgt tcaaaatgga ttaaccgagg gggctttaac
     5641 cattccgccg acgttatata cagctccaca caaggcggtg ccggaaactg ttaaagaagc
     5701 tcaatccagt ggacagttag cactgctgtt gtaattacgc gctagataat agcggcgcaa
     5761 aaaagacctc ttcgcacacc attcaattga ggatgggagg ttgggcatta cgccttgtgt
     5821 ttttttaatc accgccccag gggtttaatc gccctggggc gatgaattct tggggctttt
     5881 tcatttttat tcattggaga agtctcatgt atcaacaaaa tacacacgat gaagcaagcg
     5941 cgatcgtcaa tcaaaatatc ggcactactg atttagacga tatggagagt gaattcggcg
     6001 atgcggtaac tgaacctgac gctaacgagg gagaggtact gtcattacgg gattttattg
     6061 gcgagtttgg atcaggatta ttggcggcag ttgagcagaa taatccgccg gtttataccg
     6121 gtgcagttat caatcctaag cgccagcaca tcctaaacgc tctggcgaga aagccgttcc
     6181 cggcccaggc ggaagccgtt tatgcactta gtgcgctgtt gtttgatcaa aacgagaagg
     6241 cggccgtact gaatgctgaa atgggtactg gcaagaccat gatgtccatc agcatggctg
     6301 ccatagcgtt acaggaaggt cttaaacgga tccttgtgat cagccctccg caccttgtct
     6361 ataaatggcg cagagagatt caggagacga ttaaaggcgc tcgggtctgg gtgttaaacg
     6421 ggcctgatac cttgatgcga ctcttgaagc tgaaatcact actggttacc
//
LOCUS       DAOQGS010000197         6453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5916_length_6453_cov_4.693654, whole genome shotgun sequence.
ACCESSION   DAOQGS010000197 DAOQGS010000000
VERSION     DAOQGS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_6453_cov_4.693654"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKZ52_08885"
     CDS             <1..233
                     /locus_tag="PKZ52_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HNG59929.1"
                     /translation="GTCQQQPHAFVLDKHHRYVAGKLYPGCGNSHDMLKLSRLAQHFE
                     FFGDNRRHFGLFEGCGSSIPFTQTTPTNAACC"
     gene            242..1324
                     /locus_tag="PKZ52_08890"
     CDS             242..1324
                     /locus_tag="PKZ52_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNG59930.1"
                     /translation="MHITLVLVALLLAFSNGANDNFKGFASVWGSATLSYRSALMLSA
                     IASCSGALVALFMAHGLVASFSGKGLVSSDALSASGFMFAVACAAALTIFSATRLGLP
                     VSTTHALLGGLVGAGLGIVGAVNWAPLLQFFALPLLLSPILAAALGALLYRSVRSLPL
                     EEACVCISAEPVAALEGNGRLHMASNRNDGFGVQLGTSSGACAGATTQVAVKPWWDWL
                     HVASAMAICFARGLNDTPKLAAILLAAGAVSLNASIVAVAVFMTAGGLLCSRRVAQTL
                     SQKLSQLDTRSGLAANITTAGLVFAASHFALPVSTTHVAVGSIAGAGASSGGLHRQAL
                     VNILLSWCATLPLAALVAFVIGRLSL"
     gene            1938..3410
                     /locus_tag="PKZ52_08895"
     CDS             1938..3410
                     /locus_tag="PKZ52_08895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNG59931.1"
                     /translation="MRIFTKKQLLTGITLASSLLALSAQAAPNAVTVWEEAILDAIRT
                     TRPGPPMTARAIAMVNTCMYDAWAPYDKKAAGTMLGNSLRRPVLERTDANKTQAISYA
                     AYRAAVDLFPARKANFDALLTSQGFDPANMSMDVATPAGVGNKTCQALLDFRHHDGAN
                     QLGDLAPGAYKDYTGYVAVNTPTQINDPNRWQPLIVGGAVQPFLGAQWFKVTPFALTS
                     TDQYVPASVKPAMYGSQKYIDQAKEVIAYQANLTDYQKSVAEYWADGPNSEFPPGHWM
                     LFGLKVSARDNHSIDQDAKMFFALGNAMHDAAITAWTAKRKFDYVRPITAIHYLFANE
                     TIQGWGGPGQGTISMLGSQWQTYQASNFNTPPFPEWFSGHSTFSAAGAAVLRNFTKSD
                     VLGVSDIVRAGSSRVEPGLVPAADIKFSWATFTDAADDAGISRRYGGIHFIQGDLDGR
                     TAGKKVGNLVWRKASEYFRGVENPVCNLEFEASTQLDLAE"
     gene            3886..>6453
                     /locus_tag="PKZ52_08900"
     CDS             3886..>6453
                     /locus_tag="PKZ52_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNG59932.1"
                     /translation="MNLTACCAKTSTPTPMYLSTLQRAFFMGRRKDFDLHVHTHLYVE
                     HEFSELDINRLYRAVEILVARHPMLRAWVAPDTQVWVQQVVPVVPDVQDYSAMNGDAL
                     NLALEGRRDSLYRVDGDVELVPNIQVAVTQIASGYRVHINLDLLLLDGSSIRIVLHEL
                     SAIYDDINGHWPEKPLDFAHVAEQMAQLKASSRYQTMLKFWFERLETLPPGPQLPLRA
                     QTEAIRRSRLIRRKLIIGPERWAMLATKANKQNISATALIFSVFTWVLAYWSKQGHFS
                     VTMLMYQIRNQNMGDLSEAVANFAGTLLMEVERKAGQSFLDFCKSIHAEIFRNATKSL
                     VCGMEVLQERNRQDKSTFRAASPVAFVSMINELGNPVQPGRFQMEGDYAIAGGLETPQ
                     VLMDHQAISRPDGGVSLNWDTMDSAFEPGVIDAMFAAYSRLIDALLSDASSAGQSSSI
                     WEKTYTDLRPEAQQIKHQVYNLSQDLVPQQHLHSLFFQQAAQTPAAPYLRFLTDPADP
                     NSLQTLSYGAVRLLANRLARQLQYSQLAPGELVAVYCSKGWQQVVAAIGILAAGGAYV
                     PLDPRQPLARKQDILNRCDCRLAVTQGAWLTDAALAGLTLFDLEALPEDDLAVSMKDL
                     PARQKLSDTAYVIFTSGSTGTPKGVTLDHTGPVNSILDINRRINLTPADCLCGLSELN
                     FDLSVYDIFGAAAAGASLLIPPEGAGRDPALCGQLVQAAQVTVWNSVPALAQLFADYH
                     TSRHSWPLRLFMLSGDWIPIGLPQQLQQLRELEVLSLGGATEASIWSIYYPVRHTRSD
                     WKSIPYGYPMTNQSFYVLDQSLQPRPNQVPGELYIGGIGLAKGYWGDEEKTAQAFITH
                     "
BASE COUNT         1432 a         1875 c         1677 g         1469 t
ORIGIN      
        1 aaggcacctg ccagcagcag ccccacgcct ttgtgctgga taaacaccac cgctatgtgg
       61 cgggcaagct ctaccccggc tgtggcaata gccatgacat gctcaagctc agccgcctcg
      121 cccaacactt tgagtttttc ggcgataacc gccgccactt tggcctgttt gaaggctgcg
      181 gcagcagcat tccgtttacc caaaccacac ccactaacgc tgcctgctgc tgagattatc
      241 tatgcacata acgctcgtcc tcgtcgcgct tttgttggcc tttagcaacg gtgccaatga
      301 caacttcaaa ggttttgcca gtgtgtgggg ctcggccacc ctaagttacc gcagcgccct
      361 tatgctttcg gccattgcca gttgcagtgg cgccctggtg gcgctgttta tggcccatgg
      421 cctggtggca agtttctccg gcaagggcct ggtgagcagc gacgccctca gcgccagcgg
      481 ttttatgttt gccgtggcct gcgccgctgc cctcactatc tttagcgcca cccgactggg
      541 gctacccgtc tccaccaccc acgccctgct cggtggttta gtgggagcag ggcttggcat
      601 agtgggcgcc gtgaactggg cgcccctgct gcagtttttt gccctgcccc tattgttaag
      661 ccccattctg gctgctgccc tgggcgccct gttgtatcgc agtgtgcgca gcctgccgct
      721 ggaagaggcc tgtgtgtgta tcagcgccga accggtggcc gccctcgagg gcaatggccg
      781 gttgcacatg gccagcaacc gcaacgacgg gtttggggtg caactgggca ccagcagcgg
      841 tgcctgcgct ggtgccacaa cacaagtggc ggtaaaaccc tggtgggact ggctgcacgt
      901 cgcttcggcc atggccattt gttttgcccg cggccttaac gacaccccta aacttgctgc
      961 tattttgctg gcggcggggg ctgtttcgtt aaatgcatcg attgttgccg tggcggtgtt
     1021 tatgaccgcc gggggcctgc tctgctcccg gcgggtggcg caaaccctca gccagaaact
     1081 cagccagttg gatacccgct cgggcttggc cgccaatatc accaccgctg gcttggtgtt
     1141 tgccgccagc catttcgccc tgccggtaag caccacccat gtggcggtgg gctccattgc
     1201 cggggcgggg gccagcagcg gcgggctgca ccggcaagcg ctggtaaata ttctgctcag
     1261 ctggtgcgcc accctgccct tagccgcgct ggtggcgttt gtgattgggc gcctaagcct
     1321 ttaaggcgct atggggtgca ggtcatgcag agatgggcgc tgtgaaaccc gtcggctaaa
     1381 tatgttgcag tgtggtaggg gcaacccttg tggttgccct tgtttgccac agctaattat
     1441 cacaatccag tgctgttctg aatgcacgta ttaatcagcg agaggaatta accccagtaa
     1501 ttatttcctg ttgcaaaatt attaaaagtg aatattcact agcgggtaac agaataaacc
     1561 caggccaatt attaattgac tcgcggtgta gctatagcca gcaccgcagg caacaaaaac
     1621 tgcccacttg cccttaaaaa ttaaattgcc caggccatcg cgcctgctga tcaccgttaa
     1681 gaaaaacatc tccagcagca taaaaactgc gttatttaac ctaataaact ctgccaaacg
     1741 gcaccgaaca aaaaagtaaa taggctacat ccccttacac tgagtccgta attgttggct
     1801 gttgtcagcg ccgctaatcc accaaaatcg ccccgcaaga cccgtgcccc gtcggttaat
     1861 taacctgaga tcgttaaatt attcggttaa aacagcacac aacactcatc ccagcaatta
     1921 gaggtaggaa tctgacaatg cgtatattta cgaaaaaaca attattaacg ggcatcacac
     1981 tggccagctc cctgttggct ttatccgcac aggcggcccc caatgccgtt accgtttggg
     2041 aagaggccat cttggatgct attcgcacca cccgccccgg ccctcccatg accgcccgcg
     2101 ccatcgccat ggtaaatacc tgtatgtacg acgcctgggc gccctacgat aaaaaagccg
     2161 cgggtactat gctcggcaac agcctgcgcc gccccgtgct tgagcgcacc gacgccaata
     2221 aaacccaagc cattagctat gccgcctacc gcgccgccgt agatttattc cccgctcgca
     2281 aagctaattt cgacgccttg ttaacctctc agggttttga ccctgccaac atgagcatgg
     2341 acgtggctac gcctgccggt gtgggcaata aaacctgcca ggctctattg gattttcgcc
     2401 accacgacgg cgccaaccag ctgggggatt tagcccccgg tgcttacaaa gattacacag
     2461 gctatgttgc tgttaatacg cccactcaaa tcaacgaccc caaccgctgg cagccgctga
     2521 ttgtaggcgg cgctgtacaa ccatttttag gtgcccagtg gtttaaagtt acacccttcg
     2581 cattaaccag caccgaccaa tatgtacctg ccagcgttaa acccgccatg tacggcagcc
     2641 aaaaatatat tgaccaagcc aaagaagtta tcgcctacca agcgaattta acggattatc
     2701 aaaaatccgt agcggaatac tgggcggatg gccccaactc tgaattccca cccggccact
     2761 ggatgttatt tggcttaaaa gtatctgcgc gggataacca cagcatcgac caagacgcca
     2821 aaatgttttt tgccttgggt aatgccatgc acgatgcggc catcaccgcc tggacagcca
     2881 aacgcaaatt tgattacgtg cgcccaatca ccgcaattca ttacctattt gccaacgaaa
     2941 ccatccaagg ctggggcggc ccaggccagg gcacaatctc aatgctcggc tcgcaatggc
     3001 aaacctatca ggctagcaat ttcaacaccc cgccatttcc tgagtggttc tccggccaca
     3061 gtacttttag tgctgcaggc gccgctgtat tgcgcaattt caccaaaagc gatgtgttag
     3121 gcgtatccga tattgtgcgt gccggtagct cccgcgttga accgggttta gtgcctgcgg
     3181 ccgatattaa attcagctgg gccaccttta ccgatgcggc ggatgatgcc ggaatttcgc
     3241 gccgttacgg cggaattcac tttatccaag gcgatttaga cggccgcacc gccggtaaaa
     3301 aagtgggtaa tttagtttgg cgcaaagcca gcgaatattt ccgtggagtt gaaaaccctg
     3361 tgtgtaattt ggaatttgaa gccagtacac aattggatct tgctgaataa atattcacac
     3421 cagtgtccaa tctcgtgtgg aaacaattga aaccggcttt ggccggtttt tttatgcgtg
     3481 ctcttactac ctaggcaccg gtaatgaata cacatcattt cagacacgcc gtgaatacag
     3541 ccgtgtaggc tcgtcaaaaa tgatgtgtat tcattgcttc cgtgcagcta gtaacaatgc
     3601 ccgcagaaaa cgagacacac tgtaatcaaa aggcaaattg tccagcttct tgccactggt
     3661 taggctggtg ggcattcgtt cgcaatggcc agcgcgtctt atgactgggc cagcagaaat
     3721 acatagaaat atttcagacc gtcaaaagag aattttttgg cgagcctata cgcatttcca
     3781 ctgcgactgt atgcaatacc ctgtattcat ttccccatgt gtaacgccat gggttaagca
     3841 attattttgg attaagcgcc ttaaaggcgc ctatgtgagc aagctatgaa tttaaccgcc
     3901 tgttgtgcaa aaacctccac gcccaccccg atgtatttat ctaccttgca gcgggctttt
     3961 tttatgggcc gccgcaagga ttttgattta catgtgcaca cccatttata tgtagagcac
     4021 gaattttctg agctggatat caacaggctt taccgcgcag tggaaattct tgtggcccgc
     4081 caccccatgt tgcgcgcctg ggtggcgccg gatactcaag tgtgggtgca acaggtggtg
     4141 ccggtggtgc ctgatgtgca ggattattcc gccatgaatg gcgatgcact gaatttagcg
     4201 ctagaaggtc gccgggatag tttgtaccgg gtggatggcg atgtggaatt agtgccgaat
     4261 attcaggtgg cggtaacgca gattgccagc ggctaccgcg tgcatattaa tttggattta
     4321 ttgttattag acggctccag tattcgcata gtgctgcacg aattatccgc catttatgat
     4381 gacatcaacg gccactggcc ggaaaaaccc ctggattttg cccatgtggc cgagcagatg
     4441 gcgcaattaa aggcctccag ccgctatcaa accatgttga aattctggtt tgagaggctg
     4501 gaaaccctac cccccggccc gcaattaccc ctgcgcgcgc aaaccgaggc cattcgccgc
     4561 tcgcggttaa ttcgccgcaa attaattatc ggccccgagc gctgggccat gctcgccacc
     4621 aaagccaata aacagaatat ctccgccacc gcgcttattt ttagtgtatt tacctgggta
     4681 ttggcctatt ggtccaagca gggacatttt tccgtcacca tgctgatgta tcaaattcgc
     4741 aaccaaaata tgggggattt aagcgaagcg gtggcgaatt ttgccggcac tttattgatg
     4801 gaagtggagc gcaaagcggg ccagagtttt ttagattttt gtaaaagtat tcacgcggaa
     4861 attttccgca atgccactaa atccttagtg tgcggcatgg aagtattaca agagcgcaac
     4921 cgccaggata aatccacctt ccgcgccgca agcccagtgg cgtttgtgag catgattaat
     4981 gaattgggca accctgtgca gcccgggcgg tttcaaatgg aaggggacta tgccattgct
     5041 ggcggcttag aaacccccca ggtgttgatg gaccaccagg ccatcagccg cccagatggg
     5101 ggtgtatccc tcaactggga caccatggac agcgcctttg agccgggcgt tatcgacgcc
     5161 atgttcgcag cctacagccg cctgattgat gcgctattaa gtgatgccag cagcgcaggg
     5221 caatccagca gcatctggga aaaaacctat accgacctgc gcccggaggc ccagcagatt
     5281 aaacaccagg tgtataacct cagccaagat ttagtccccc agcagcacct gcacagcctg
     5341 ttcttccagc aggccgccca aacccccgcc gctccttatc tgcgttttct tacggaccct
     5401 gcggatccta acagcctaca aaccctcagc tacggcgccg tgcgcctcct tgccaaccgc
     5461 ttagcccgcc agctgcagta cagccagctg gcgccgggtg agctggtggc ggtgtattgc
     5521 agcaagggct ggcagcaggt ggtggcggcc attggcatcc tggccgccgg gggcgcctat
     5581 gtgcccctgg acccccgcca gcccctggcc cgcaaacagg acattttaaa ccgctgcgat
     5641 tgccgcctgg ccgttaccca aggggcctgg cttacggatg cagccctcgc gggcctcacc
     5701 ctcttcgatc ttgaagcgct gcccgaggat gatttggccg tgagcatgaa ggacttgcca
     5761 gcgcgccaga agctcagtga caccgcctac gtgattttca cctccggttc caccggcaca
     5821 cccaagggtg taaccctgga tcacacaggg ccagtcaaca gtattttgga catcaaccgc
     5881 cgcattaacc tcaccccagc ggactgtttg tgcggcctgt cggagctgaa ttttgacctc
     5941 tcggtgtacg acatcttcgg cgcagcagca gccggggcga gcctattgat tcccccggaa
     6001 ggcgcaggcc gggacccggc cctctgcggc caactggtgc aggccgcgca ggtgacggtg
     6061 tggaattctg tgcccgccct ggcgcagtta tttgccgact accacaccag ccgtcactcc
     6121 tggcccctgc gcctgtttat gctcagcggc gattggattc ccattggcct gccccagcaa
     6181 ttgcagcagt tgagggagct tgaggtattg agcctgggcg gcgccaccga ggcctctatc
     6241 tggtccattt attaccccgt gcgccacact cgcagcgatt ggaaatccat cccctacggc
     6301 taccccatga ccaaccagag cttttatgtg ctggatcaaa gcctgcagcc gcgccccaac
     6361 caagtgccag gcgagctgta tatcggcggc attggcctag cgaagggtta ttggggggat
     6421 gaagaaaaaa ccgcccaggc gtttatcacc cac
//
LOCUS       DAOQGS010000198         6451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5918_length_6451_cov_4.641338, whole genome shotgun sequence.
ACCESSION   DAOQGS010000198 DAOQGS010000000
VERSION     DAOQGS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5918_length_6451_cov_4.641338"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKZ52_08905"
     CDS             complement(<1..423)
                     /locus_tag="PKZ52_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNG59933.1"
                     /translation="MLKVEVYRYNPETDAKPYMKTYQVDTGGKDLMVLDVLELLKGQD
                     ETLAYRRSCREGVCGSDGMNISGKNGLACVTPLSSCVRNNTLVLRPLPGLPVIRDLVI
                     DMSQFYEQYRKIEPYLQNHTPAPAIERLHGKRGDGAGGI"
     gene            complement(441..2213)
                     /gene="sdhA"
                     /locus_tag="PKZ52_08910"
     CDS             complement(441..2213)
                     /gene="sdhA"
                     /locus_tag="PKZ52_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNG59934.1"
                     /translation="MASLRTMSFDGIVIGGGGAGMRAALQMAQSGFKTAVITKVFPTR
                     SHTVSAQGGITCAIASDDPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSVGPEAVFELE
                     HMGLPFSRTEKGRIYQRPFGGQSKDFGKGGQAARTCAAADRTGHALLHTLYQQNVKAN
                     TVFLNEWFAVDLVKNQDGAVVGVIAINMEDGETVYIRSKATVLATGGAGRIYASTTNA
                     HINTGDGVGMALRAGFPVQDIEMWQFHPTGIAGAGVLVTEGCRGEGGFLVNKDGERFM
                     ERYAPNAKDLAGRDVVARSMVREIIEGRGCGPNGDHVLLKLDHLGEDALNAKLPGILE
                     LSRTFAHADPVKEPIPVVPTCHYMMGGIPTNIHGQALTLDSNGRDQVIPGLYACGEVA
                     CVSVHGANRLGGNSLLDLVVFGRASGLFIEKALREGIELREASQADLDQAMSRLNRLN
                     NSTGGESATVLRKELQTIMQNYFGVFRTGEFMQKGIEQLAALRSRIEQIGLSDKSAAF
                     NTARIEALEVQNLFEVAEATAIAAEERKESRGAHAREDFEERDDTNWLCHSIYFPSAK
                     RVAKRAVNFAPKTVEAFEPKVRTY"
     gene            complement(2217..2585)
                     /gene="sdhD"
                     /locus_tag="PKZ52_08915"
     CDS             complement(2217..2585)
                     /gene="sdhD"
                     /locus_tag="PKZ52_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNG59935.1"
                     /translation="MVRAATSFGRSGVSDWIVQRFSAVILAAYTVFIAGFLIAHPGLD
                     YATWHALFGNLAVKIFTLLALLSVAAHGWIGLWAVITDYLTARVLGCKALPLRMFILA
                     TYAVVTFAYVAWGIQILWGN"
     gene            complement(2579..2953)
                     /gene="sdhC"
                     /locus_tag="PKZ52_08920"
     CDS             complement(2579..2953)
                     /gene="sdhC"
                     /locus_tag="PKZ52_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNG59936.1"
                     /translation="MNKNRPVNLDLATITLPVTGYVSILHRISGVVMLAGVLGLLWLL
                     DATLSSEAGFAAVQKCLSSPGAKIVIWGIFSALAYHLVAGIRHLIMDMGVGESLEGGR
                     LGAKLVLVIAAVLIVGLGVWLW"
     gene            3944..5233
                     /gene="gltA"
                     /locus_tag="PKZ52_08925"
     CDS             3944..5233
                     /gene="gltA"
                     /locus_tag="PKZ52_08925"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNG59937.1"
                     /translation="MTDKKAQLTVEGLSSPIELPVYSGALGPDVIDVRGLVSKGYFTY
                     DPGFVSTASCESKITFIDGDKGVLLHRGYPIETLAEKSDYLETCYLLLNGELPTPEQN
                     AEFKHIITHHTMVNESIARFLLGFHYDSHPMAMLCGVVGALSAFYHDAMDISDPQHRI
                     ISAHRLIAKMPTLAAMCYKHRVGQPFVYPNNKMSYAENFLHMMFGTPCDEPYVSPVLA
                     RAMDRIFLLHADHEQNASTSTVRLAGSSGANPFACIAAGIATLWGPAHGGANEAVLDM
                     LEEIGDVANIEKYVAKAKDKNDPFRLMGFGHRVYKNFDPRAKVMKQTCDEVLTELGLE
                     NDPLLKIAKRLEQIALEDPYFIEKKLYPNVDFYSGIIMKAIGIPTEMFTAIFAVGRTV
                     GWISHWHEMVTEGLKIGRPRQLYTGYPTREYPARDKR"
     gene            5386..6351
                     /gene="glk"
                     /locus_tag="PKZ52_08930"
     CDS             5386..6351
                     /gene="glk"
                     /locus_tag="PKZ52_08930"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNG59938.1"
                     /translation="MYPSIVADIGGTNARFALVSGKANDQFTFSDIHILPAGNYANFS
                     DALRAYINLVPGLQPKAICAAIAGPVVGDKVRMTNLNWAFSCKAVAEEFGLARFIAMN
                     DFAAVAAAAGDIHPEHLINIKPGRADPHGNKAVFGPGTGLGVAGVVNYNGKWLPLPSE
                     GGHVNLAPSDAFEADVIKAAMALFGHVSAEVFISGPGLVHLHQAIAAVQGVAPGAFKP
                     ADITQHALAGSDASCLLTLQTFCSLIGSFAGNLALTYGATGGVYMAGGILPRFADFLR
                     TSSFSEKFTAKGAQKGYVADVPADLIVHPEVAFAGAAAWLEQHLT"
BASE COUNT         1568 a         1843 c         1599 g         1441 t
ORIGIN      
        1 aatgccaccg gcaccgtcgc cacgcttgcc gtgcaaccgc tcgatggctg gcgcaggcgt
       61 gtggttttgc aaatagggct caattttgcg gtactgctcg tagaactggc tcatatcaat
      121 caccagatcg cgaataaccg gcaacccagg tagcgggcgc agcaccagag tattgtttct
      181 tacgcagctg gatagtgggg tcacacaggc caggccattt ttgccggaga tattcatacc
      241 atccgagccg cacacgcctt cgcggcatga gcgacgatag gcgagggttt catcctgccc
      301 cttcaataac tcaagcacat ccaacaccat caagtctttc ccgcccgtat ctacctgata
      361 ggttttcatg tagggcttgg cgtcggtctc tgggttgtag cggtacactt ctactttcaa
      421 cataaaatca cctgccccac ttagtaagta cgcactttag gttcaaacgc ttccaccgtt
      481 ttaggtgcaa agttaacggc acgcttagct acacgtttag cgcttgggaa ataaatggag
      541 tgacacagcc agttggtgtc atcgcgctct tcgaagtcct cccgcgcatg ggcgccgcgg
      601 gattctttgc gctcttcggc ggcaatggcg gtagcttccg ccacttcaaa caggttttgc
      661 acttccagcg cttcgatgcg cgcagtgtta aacgcagcgc ttttatcgct aaggccaatt
      721 tgttcgatgc gcgagcgcag ggccgccaac tgctcaatac ccttctgcat aaattcaccg
      781 gtgcggaaca caccgaagta attctgcatg atggtttgca gctctttgcg cagaactgta
      841 gccgattcgc cgccggtgct gttatttaag cggttgagac ggctcatggc ttggtccaaa
      901 tccgcctggc tggcctcgcg cagctcaatg ccttcgcgca gggctttttc aataaataag
      961 ccggaggcgc ggccaaatac aaccagatcc aacagcgagt tgccgcccaa acggttggcg
     1021 ccgtgcacgg atacgcaggc cacttcacca caggcgtata aaccggggat gacttgatcg
     1081 cgaccattgc tgtccagcgt taatgcctgg ccgtgaatat tggtgggaat accacccatc
     1141 atgtaatggc aagtgggcac gacgggaata ggttctttga ccggatcggc gtgggcgaag
     1201 gtgcgagata attccaaaat acccggcaac ttagcgttga gcgcgtcttc gcccaggtga
     1261 tcgagcttga gcaatacgtg gtcaccgttg gggccgcagc cgcggccttc gataatttcc
     1321 cgcaccatgg agcgcgccac cacatcgcgg cctgccaagt ctttagcatt gggagcgtag
     1381 cgctccataa aacgctcgcc atctttattg accaaaaagc cgccttcacc gcggcaaccc
     1441 tcggtaacca aaacccccgc accggcaata cccgtggggt gaaactgcca catctcgatg
     1501 tcttgcaccg ggaagcccgc gcgtagagcc atgcctacgc cgtcgccggt attgatgtga
     1561 gcattggtgg tggatgcgta gatgcgcccc gctccaccag tggccagcac agtcgcttta
     1621 gagcggatat aaacggtttc gccgtcttcc atattaatag caataacacc caccacggcg
     1681 ccatcttggt tcttaaccaa atcgacggca aaccactcgt tcaagaaaac agtattggct
     1741 ttgacgtttt gctgatagag ggtatgtaac agggcgtggc cggttcgatc cgctgccgca
     1801 caggtgcgcg cagcttgacc acctttgccg aaatctttgg attgaccgcc aaatggacgc
     1861 tggtagatgc ggcctttttc agtgcgcgaa aacggcaggc ccatgtgctc aagctcaaat
     1921 acggcttcgg ggccgacgga acacatgtac tcaatggcgt cttggtcgcc gatgtaatcg
     1981 gagcccttca ccgtgtcata catatgccag cgccaatcat cgttgggatc gtcactggca
     2041 atggcacagg tgatgccacc ctgggctgaa actgtatggg agcgggttgg aaatacttta
     2101 gtaattactg ctgttttgaa ccctgactgc gccatctgca gtgcggcgcg cataccggca
     2161 ccaccgccac caatcacgat gccgtcaaaa ctcatggtac gaagacttgc cataacttaa
     2221 ttaccccaca aaatctgaat tccccaggcc acgtaggcga aggtgacaac ggcgtaggtc
     2281 gccagaataa acatacgcag gggcagtgct ttacagccta aaacccgtgc ggtgaggtaa
     2341 tcggtaatga ccgcccaaag gccgatccaa ccgtgcgcgg ctacagataa aagcgccaac
     2401 agagtaaaaa tttttactgc caaattgcca aatagggcat gccaggtggc gtagtccagg
     2461 cctgggtggg cgattaagaa acccgcaata aacactgtgt aagcagccaa aataactgcg
     2521 ctaaaacgct gaacaatcca gtcgctaacc ccactgcgac cgaaactggt tgccgccctt
     2581 accataacca aacccccaaa cctacaatta ataccgctgc gataaccaac accaatttag
     2641 cgcctaaacg accgccctct aaggactcac cgacgcccat atccataatc aagtggcgaa
     2701 tgcccgccac caaatggtag gccagggccg agaagatgcc ccaaattaca atcttggctc
     2761 ccggcgatga caggcatttt tgcactgcgg cgaaccccgc ttctgaactg agggtcgcat
     2821 cgagcaacca gagcaagccc agcacccccg ccagcatgac tacaccacta attcgatgaa
     2881 ggatggagac atagccggtc acaggcagcg tgatcgtggc cagatcgaga ttgacgggtc
     2941 tgtttttatt cacagtattg gtaccttcgt gtcaggattt aaattgattg cttacccaag
     3001 cggcagctga ctgcaaagcc ctgcgcacgc aggcacaatt ggcgaatata gccgctgaaa
     3061 cactcaaccc gttcattgca gcagccgaac taacttggct gcaatcgtgc ttacggctta
     3121 ctaaaagcac tgtttgccga tataaatacg catcacacgc acaaaaaagg caaacactcc
     3181 ccttcgggtg atcttaactt agcaccctca cagctcgacg aaacaaacga aaagtggagc
     3241 tttgcgatcg caaaaacggc gcgaattata gatatgcaag gcagaaaata caaacgaaca
     3301 ttgcggtaaa accctttatt tctctgggct caacaggcaa aaacgcaccc atattgggat
     3361 ttttcacaaa tttcgcaccg cgaatgcgca aaatggaata ccatcggtac tcaagcggat
     3421 ttaactttag ctttcaccaa tctctgcttc cttgatttgg tgctttttct ctatactcca
     3481 ctcaacgcta tttaagcgac agctagcccg ttcaaagcag tagtgcctgc atgaggagcc
     3541 ttaattaaat aggtactcct gcgtgtgacg gtacgcagat ggcgcgcagc taaagccgcg
     3601 tgattttatt tgcgtgtatg ctcagaacct agatagaacg caaatagata ctaagtagct
     3661 cagtaaaaag aagaaacaca gcgaaaacag cagcccggta aaatcctggt ggcgaataat
     3721 aatcaggcaa atagattaga caaagagcag acagttaccc agttagaaat aacccattgt
     3781 tttgtactgg ccactttcgg aaaggatagg ttttagataa ttcctccgct gcgcctgaat
     3841 cggcctgttt actgctatga caacgcacaa ccaggcatag gggccgggtt attgcccgag
     3901 cctaagcacc cataacacta ctcaacatct ataggagtcc gtaatgactg acaagaaagc
     3961 tcaacttacg gtcgaagggt tgtcttcccc cattgagttg cccgtctact ctggcgccct
     4021 cggcccagac gtgatcgacg tacgcggctt agtgagcaaa ggctacttta cttacgaccc
     4081 gggctttgtt tccactgcct cgtgtgaatc gaagatcaca tttatcgacg gtgacaaggg
     4141 cgtcctactg catcgcggtt atcctatcga gacgctggcg gaaaaatctg attatcttga
     4201 aacctgctat ttattgctca atggcgaact gcccacccca gagcaaaacg ccgagttcaa
     4261 acacatcatc acccaccaca ccatggtgaa cgagtccatc gcccgctttt tactgggctt
     4321 tcactacgac tctcacccca tggctatgct gtgcggcgta gtgggcgccc tgtcggcgtt
     4381 ttaccacgat gcgatggaca tctccgaccc acagcaccgt atcatctccg cccaccgttt
     4441 aattgccaaa atgcccaccc tggcggccat gtgttacaaa caccgcgtgg gccagccctt
     4501 cgtgtaccca aacaacaaaa tgagttatgc agagaacttc ctgcatatga tgtttggcac
     4561 cccctgcgat gaaccttacg taagcccggt gttggcgcgc gctatggatc gcatcttcct
     4621 attacacgcc gaccacgaac aaaacgcttc cacctctact gtgcgcttgg caggctcctc
     4681 gggcgccaac ccatttgcct gtattgcagc gggtatcgcc accctgtggg ggccggccca
     4741 cggcggcgcg aacgaagccg tgttagatat gctcgaagaa attggcgatg tagccaatat
     4801 tgaaaaatac gtagccaagg cgaaagacaa aaatgatccg ttccgcctga tgggcttcgg
     4861 ccaccgggtg tataaaaact ttgacccccg cgccaaagtg atgaagcaaa cctgtgacga
     4921 ggtattaacc gagcttggtt tggagaacga tccactgctg aaaatcgcca agcgcctcga
     4981 gcagatcgcc ctggaagacc cttacttcat cgagaaaaaa ctctacccca acgtggattt
     5041 ctactcgggc atcatcatga aagccattgg tattcccacc gagatgttca ccgctatttt
     5101 tgctgtgggc cgcaccgtgg gctggatctc ccactggcat gaaatggtca ccgaaggttt
     5161 aaaaattggc cgtcctcgcc agctgtatac aggttacccc acgcgggaat acccagctcg
     5221 cgacaagcgc taatggcaaa tatctagttt ggcgccaacg ccaaacgcca cacaccctta
     5281 aaggcccgca agggcctttt ttgtgtgcat gctttcaggt caccttgacc accttaagcg
     5341 gcttcactat tgtggccgat ttttttagcg ccctggataa cctctatgta cccttctatc
     5401 gtcgccgata tcggcggaac taacgcccgt tttgctctgg tgagcggcaa agccaacgat
     5461 caattcacct tcagcgatat tcatatccta cccgctggca actacgccaa tttcagcgat
     5521 gccctgcgcg cctacatcaa tttagtgccc ggcctgcaac ccaaggccat ttgcgccgcc
     5581 atagcaggcc ccgtggtggg cgataaggtg cgcatgacga atttaaattg ggcgttttcc
     5641 tgcaaggccg ttgctgaaga atttggcctg gcgcggttta tcgcgatgaa tgatttcgcc
     5701 gctgtggccg ccgccgctgg ggatattcac ccggagcatc tcattaacat caaacccggc
     5761 cgcgccgacc cccacggcaa taaggcagtt tttggcccag gcactggcct gggtgtggcg
     5821 ggcgtggtga attacaacgg caagtggctg cccctgccca gcgaaggcgg ccatgtgaac
     5881 ctagccccta gcgatgcctt tgaagccgat gttattaagg ctgccatggc gttatttggc
     5941 cacgtgtcgg ccgaagtatt tatttccggc cccggccttg tgcatttgca tcaagccatt
     6001 gccgctgtgc agggcgtggc gcccggcgcg tttaaacccg ccgatattac gcagcatgct
     6061 ctggcaggct ccgatgccag ctgcctgtta actctgcaaa ccttttgcag tttgatcggc
     6121 tcctttgcgg gcaacctagc cctcacctat ggcgccaccg gtggagtgta tatggcggga
     6181 ggtattttgc cgcgctttgc agatttcttg cgcaccagca gctttagcga aaaattcacc
     6241 gccaagggcg cgcaaaaagg ttatgtggcc gatgtgccag cggacttaat tgttcaccct
     6301 gaagtagcct ttgccggtgc cgccgcctgg ttagaacaac acttaactta acgcctatat
     6361 acgtaacgct atgcacgccc gcaccgaggg cctcctcgcc atttacacgg ccatggtgtt
     6421 attggccatc aatggcgtgt tatccaaaac c
//
LOCUS       DAOQGS010000199         6450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5921_length_6450_cov_5.241126, whole genome shotgun sequence.
ACCESSION   DAOQGS010000199 DAOQGS010000000
VERSION     DAOQGS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5921_length_6450_cov_5.241126"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1069)
                     /locus_tag="PKZ52_08935"
     CDS             complement(374..1069)
                     /locus_tag="PKZ52_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNG59939.1"
                     /translation="MIFRQLFERESSTYTYLIGCSQTRAAVLIDTVKSQLPVYLQLLA
                     ELNLNLVAVLDTHTHADHITAAGALRDTTGALTYLGAEANSNCVSKPLQHGAIIPVGQ
                     LAITALHTPGHTPDSYSFILKVDNQTYAFTGDTLLIRGTGRTDFQNGSAASQYSSLFN
                     CLLQLPENTWVYPGHDYKGWTVSTIGEEKAHNPRLQVKTPADYINLMDGLNLPNPQLM
                     DVAVPANQACGQG"
     gene            complement(1119..1940)
                     /locus_tag="PKZ52_08940"
     CDS             complement(1119..1940)
                     /locus_tag="PKZ52_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG59940.1"
                     /translation="MLLLIGLAIGLILGLTGAGGSVFAVPLLMLLAGTPMLEATGIAL
                     GAVAAASLFGSLRNRASGMILWVPGLLLAATGMLSAPIGKWLALQTPPQVLILSFNAL
                     AVLIALRMGYSAAVNPEQAQVVRASVHPPVAPGGALCPLSPSGQFQLRLPCVSGLLMG
                     GALVGLLSGLLGVGGGFLIVPLLLALSPIGMPQAVSTSLVVITLVSSAGFISHLTLHQ
                     PLDWSLLGLITLGSLLGMALSQGLAKKIANARLQKLFAASLIAVALISSKTLIFP"
     gene            complement(1965..2489)
                     /locus_tag="PKZ52_08945"
     CDS             complement(1965..2489)
                     /locus_tag="PKZ52_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG59941.1"
                     /translation="MQRIPPLDLISRKTADTCILDVRTAAEVKAASLPDCLHIPLHEL
                     TPERLQAVLSKSGKDGRCIYLLCQAGRRAEMAAEQLTGKINAELCIIEGGMEAVKQSN
                     IQLNAPGPKVISLERQVRIIAGLLVLTGAALGAWVNPAFYGLSAFVGAGLTFAGISDI
                     CLMGILIAKMPWNT"
     gene            complement(2515..2829)
                     /locus_tag="PKZ52_08950"
     CDS             complement(2515..2829)
                     /locus_tag="PKZ52_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNG59942.1"
                     /translation="MNDTFTNNPAMQAHAAKAASLLKALANEHRLMIMCTLIDGELCV
                     GDLNERVPLSQSALSQHLASLREAGLVRTRKESQTVYYAVNGNEASKIIAVLQSIYCP
                     PA"
     gene            3669..5024
                     /locus_tag="PKZ52_08955"
     CDS             3669..5024
                     /locus_tag="PKZ52_08955"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNG59943.1"
                     /translation="MAKHVNIRKYSKKSRFAALVLLSVSLLGCGSGGSSSGAGVSSSS
                     SSSSSSSSSSSGSPSSSSSSSSSSSTTSSTGAPVLTPEPNAPQQTRAFPEQTRAPEQL
                     SSFSVQATLITDKLSSPWGITRMPDGRWLITQKGGSLVIVTTSGQISANISGVPTVYS
                     GGQGGLLDVSLDPQFATNRYVYLSYAEPREDGKNGTSVGRATLSTDEKRLENWQVIFR
                     QMPAWNSTLHFGSRLAWDKNGLLYVTLGERSLPESRVFAQDISTTLGKVVRIKADGTI
                     PADNPFVATTNARPEIWSYGHRNVQGAAIHPITGELWTMEHGPRGGDEVNRPQAGKNY
                     GWPIITYGEDYSGAPIGDGITAKAGMEQPIYYFDPVIAPGGINFYRGSLFSGWENNLL
                     ISSLTPGGVVRLVFKDNKVVGEERVAKQLGRVRDLDVAEDGSIWVITDSGQLNRLTPK
                     T"
     gene            complement(5499..6281)
                     /locus_tag="PKZ52_08960"
     CDS             complement(5499..6281)
                     /locus_tag="PKZ52_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HNG59944.1"
                     /translation="MAQLILLDLDGTLYVDNQILPGAPEALAQLRETHALAFITNTTT
                     QTAASLVAKLARMGIEIGQDELFTPVQATWQYLQQWQIEQGRSPRVWPLVAHALEADF
                     AAYTFDDKTPDVVVLGDIGERWNLGLINQLFQCLHQGSQLIALHKNRYWQTAEGLKAD
                     IGFFVAGLEYVTGQAAKIMGKPSGEFFQQALARFGCTVELSFMVGDDIDSDVGGAQAA
                     GIKGVLVQTGKYRDHYCAQSEVKPWAIIRDISVLPGLLSNSQ"
BASE COUNT         1533 a         1650 c         1760 g         1507 t
ORIGIN      
        1 tggtagccgt catgctgccg ttggcatcgt aggtataact gttgccgccc gcgcttccaa
       61 ccctctcctt gaaaagggga gggggacata gcgcggagac tcacgattta tctttcattt
      121 atcaagacgc agccttctgc tcctcgtaag gataagcata ataaaccacc gttatcacca
      181 ccaaagtcaa caccgttgaa tccatcaaac taaaaactaa cgattccgcc agcccgccaa
      241 atataggggt gtggaaaata aaaaagccgc cgtcaaagac ttccgaataa taattgttgt
      301 gatcctgcag atcggccagg caacacaatc acgtctttgt cctgcacctg ctgcctctca
      361 cgggagggga attttacccc tgcccacagg cctggtttgc tggcaccgct acatccatta
      421 attgcgggtt gggtaaattt agcccgtcca tcaggtttat ataatccgcc ggggttttta
      481 cctgcaaacg ggggttgtgg gctttttctt cgcctatggt gctcaccgtc cagcctttgt
      541 aatcgtggcc ggggtatacc caggtgtttt ccggtaattg cagcaggcaa ttaaataaac
      601 tgctgtattg gcttgcggcg ctgccgtttt gaaaatccgt gcggccggtg ccgcgaatta
      661 atagggtatc accggtaaag gcgtaggttt ggttgtctac ttttaaaata aaactatagg
      721 aatccggtgt atggcctggg gtgtgtaaag cggtgatggc cagttgcccg acgggaatga
      781 tcgcaccgtg ctgcaggggt ttggatacac agttggagtt ggcctcggcg cctaaatagg
      841 tgagtgcgcc ggtggtatcg cgcagggcgc ctgcggcggt gatgtggtcg gcgtgggtgt
      901 gggtgtctaa cacggccacc aagtttaaat ttaattccgc caataattgc aaatacaccg
      961 gcagctggct ttttaccgta tcaattaaca ccgccgcgcg ggtttggctg cagccgatta
     1021 aataagtgta ggtggaggat tcgcgctcga ataattgccg gaaaatcata atgcttgcct
     1081 cttaaattaa tgattaaaac tctgtgtgca tgcgcgtgtt aagggaaaat cagggttttt
     1141 gatgaaatga gcgcaacggc aattaagctt gcagcaaata gtttttgcag ccgggcattg
     1201 gcaatttttt tggctagacc ttggcttagg gccatgccca atagactgcc cagggtgatt
     1261 aagcctaata agctccaatc caggggctgg tgcaaggtta agtggctgat aaaccccgcg
     1321 ctgctcacca gggtaattac caccaatgag gtacttaccg cctggggcat gccgatgggg
     1381 ctaagcgcca gcagtagcgg cacaatcaaa aacccgcccc ccacgcccaa caagccggac
     1441 aataaaccca ccagggcgcc gcccatcaat agcccgctaa cgcagggcag gcgcagttga
     1501 aattgcccgc tggggcttag ggggcagagg gcgccgcccg gcgccaccgg cgggtgcaca
     1561 ctggcgcgca ccacttgggc ctgttccggg ttaaccgccg cgctgtaccc catgcgcagg
     1621 gcaatcagca ccgctagggc gttaaagctc aaaattaaaa cctgcggcgg tgtttgtagg
     1681 gccagccatt tacctatagg tgcgctgagc ataccggtgg cggcgagcag caggcccggc
     1741 acccaaagga tcatgccgga cgcccgatta cgcaggctgc cgaacaggga cgccgccgct
     1801 acagcgccga gggcaatccc ggtggcctcc agcatggggg tgccggccag taacatcagc
     1861 aggggcacgg caaataccga tccccctgcg ccggtcagcc ccaagatcag cccaatggca
     1921 agtccaatca acagcagcat aaaaatcccc taagcgttaa tggactaggt attccagggc
     1981 attttggcga tcaatatgcc catgaggcag atgtcgctga tccccgcgaa ggtaagcccc
     2041 gccccaacga aggctgatag gccataaaaa gccgggttga cccaggcgcc cagggctgcg
     2101 ccggttagca ccaagagccc ggcgataatg cgcacctgcc gctccaagga aatcaccttg
     2161 gggccgggcg cattgagctg aatattggat tgtttaaccg cctccatgcc gccttcgatg
     2221 atgcacagct cggcgtttat tttgcccgtc agctgctcgg cggccatctc tgcccggcgg
     2281 cccgcttggc acagcaggta aatacagcgg ccatctttac cgcttttgga cagtactgcc
     2341 tgcaggcgct cgggggtgag ttcatgcagg gggatgtgca aacaatcggg caggctggcg
     2401 gcttttacct ccgctgcggt gcgtacatcc aaaatgcagg tgtcggcggt tttgcggctg
     2461 attaaatcaa gagggggaat gcgttgcatg gcgggctcct cagcggttgg gcaattaagc
     2521 gggtgggcaa taaatggact gtaaaactgc gatgattttg ctcgcctcat tgccgttcac
     2581 cgcgtaatac acggtttggg attctttgcg ggtgcgcact aaacccgcct ctcgtaggct
     2641 ggctaggtgt tgggagaggg cagactggct caggggcacc cgctcgttta gatcgcccac
     2701 gcacagctcc ccatcgataa gggtgcacat aatcattagc cggtgttcgt tggccagggc
     2761 ttttaacagg ctggcggctt tagcggcgtg ggcctgcatg gcggggttgt tggtgaaggt
     2821 atcgttcatg gcagctcgga ttctggaggg gttttgttga gtatatttga taatattaaa
     2881 ttagcaatac ctaaattaat aaaatcttta ttaggtttgt ttgatgtggt ggcaaccgcc
     2941 tcgcgagctg agggtttagt gcgcttggga aatcagggcg tgtgtctctg tggctgccta
     3001 acctagaggt atgggtgtcc ggtcaataaa ccccattgtg gtcgtttgct atcgggcctg
     3061 tgtcatcctg cgtgcgagat gacggcgatt aaatgcggaa gagacgttac agaaatttat
     3121 ttaattctaa gttcaggccc taaggccggg cttagccttt agcgcgatgc tcaggcaaaa
     3181 ctaaaaaatc acctttattt cccgtctaga acaggcgtgg tagggctgaa acaggcaatg
     3241 ccaaaatcgt cccgctaaga agacaacatc gaccggacac ccatgcctta ttaataatcc
     3301 taaaaaacgc agttgaactc tactgcggcc gcctaatact tgaatctgca cgcttttttg
     3361 tcaaaatttg actcaccgta gtggtgacta ctgctttcgc caaattttga caaaaaagcg
     3421 cccatattca gcgtcttagg caccctcgct acgagttcaa ctgcggtttt taggataatg
     3481 gccgtcgcca aatactgaaa aaaccgcaaa tatttagtgt cttgacacct gccttgcggg
     3541 gccgctgagg ttttcaggat taaatgtttt atctgtgcac ttgtagccgt tcgcttgcat
     3601 ggtaagtact tatggggcgg ggtatgatca aagccgtttt ttacgttcat tgatgcgagg
     3661 aataacccat ggctaagcat gtgaatatcc gtaaatacag caaaaaaagc cgttttgctg
     3721 cgctggtgtt gttgagtgtt agtttattgg ggtgtggcag cggtggcagt agtagtggcg
     3781 cgggcgtgag cagcagttca tccagtagct catccagtag cagctcatca agcggtagtc
     3841 cctcttcaag tagctccagc agttccagca gcagtaccac cagcagtacc ggtgctccgg
     3901 tactcacccc tgagcccaat gcgccacaac aaacccgcgc gtttcccgag caaacccgcg
     3961 cgccggagca attgagcagt tttagcgttc aagccacttt aattaccgat aaattaagca
     4021 gcccttgggg cattacccgc atgcctgacg gccgctggtt aattacacaa aagggcggca
     4081 gcctggtgat tgtcacaacc agcgggcaga tttccgccaa tattagcggg gtgcctacgg
     4141 tatacagcgg cggccagggc ggtttgttgg acgtgagttt agacccgcaa tttgccacta
     4201 atcgctatgt atatttaagt tatgcagaac cccgcgaaga cggtaaaaat ggcacttctg
     4261 taggccgcgc aaccttatcc accgatgaaa aacgcttgga aaattggcag gttattttcc
     4321 gtcaaatgcc cgcctggaat tccaccctgc attttggttc gcgcctggcc tgggataaaa
     4381 acggcctgct gtatgtaacc ctcggcgagc gctcgctgcc ggaatcccgc gtttttgccc
     4441 aggatatcag caccacttta ggcaaggtgg tgcgcatcaa agccgacggt actatccctg
     4501 cggataaccc ctttgtggca acaaccaatg cccgcccgga gatttggtcc tacggccacc
     4561 gcaatgtaca aggcgccgcc attcacccta taacagggga attatggacc atggagcacg
     4621 gccctagagg cggcgatgaa gtcaaccgcc cgcaagcggg taaaaattac ggctggccaa
     4681 tcattaccta tggcgaagat tacagcggcg cccccatagg ggatggcatt acggctaaag
     4741 cgggtatgga gcaaccgatt tattacttcg acccggtaat cgcccccggc ggcattaatt
     4801 tttatcgcgg cagtttattt agcggttggg agaataattt attaattagc tcattaactc
     4861 cgggcggtgt ggtgcgcttg gtatttaaag acaataaagt ggtgggcgaa gagcgcgtgg
     4921 caaaacagtt gggccgcgtg cgggatttgg acgtggctga ggatgggtct atttgggtaa
     4981 ttacggatag tggccagtta aaccgtttaa ctccaaaaac ctaactgctc ctcgttccca
     5041 cgctcagcgt gggaacgcca acccgtttga aatacacgat tgtttattta ggccagagtt
     5101 aactattgga cacaccctat taccctcttt cgcacggcaa tgtcattaag ttgagggggc
     5161 gtaggggcaa cccttgtggt tgccctgcct gtgtctcggt tatcgatcag gagcgggcac
     5221 ccacaagggg tgcccctact gctgtttgcg agtcagtaag tcctcaactt attaacgaag
     5281 cggaagcaag gaatacctat tatttcaaac cgtcaaaaag aagaaccttt ttgacgagcc
     5341 tacaaggatg tattcacagc gtgtttgaaa taataagcat tcatcaccgc aggtgggctc
     5401 gcagtactaa agcaccgcca gatagtttcc cgacactgcg ggaattacat taaaaaggtg
     5461 ctgataaaaa ggcgctaaat tgtcaccgcc agaattactt actggctatt gctcagcaaa
     5521 cctggcaaca cactaatatc gcgaataatc gcccagggtt taacctctga ctgggcgcaa
     5581 taatggtcgc ggtatttacc ggtttgtact aaaacgcctt ttatccctgc cgcctgggcg
     5641 ccgcctacgt cggaatctat atcatccccc accataaaac tcagctcgac tgtgcaacca
     5701 aaacgcgcta gcgcctgttg gaaaaattcc ccgctgggtt tacccataat tttggcggcc
     5761 tggccggtca cgtattccag ccccgccaca aaaaaaccta tatcggcttt aaggccctcg
     5821 gcggtttgcc agtagcggtt tttatgcagg gcaattaatt ggctgccctg gtgcaggcat
     5881 tggaataatt gattaattag gcctaaattc cagcgctcac ctatatcacc taataccacc
     5941 acatcggggg ttttatcatc aaaggtgtaa gcggcaaaat ccgcctctag ggcatgagcc
     6001 accaggggcc acacacgggg gctgcggccc tgctcgatct gccattgctg caggtattgc
     6061 caggtggcct gcaccggcgt gaagagttcg tcctgcccaa tttcaatccc catgcgggcc
     6121 aatttggcca ccaagcttgc ggcagtttgg gtggtggtgt tggtgataaa cgccaaggcg
     6181 tgcgtttctc gcagttgagc taaagcctct ggggcgccgg gcagaatctg attatccacg
     6241 tagagtgtgc cgtcgagatc taagagtatt aattgcgcca tagcctaagc ctcgtgggta
     6301 aattgacgaa aacgggcgat gggcaattac acgcaataaa tatcccaagc gggcagattg
     6361 tgtttattta aaagcgggaa aaggtggcag gggcggatat ggcggtgaaa taaggcccct
     6421 cagcgagttc ggggccgatg gaaaacccat
//
LOCUS       DAOQGS010000200         6447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5925_length_6447_cov_4.516114, whole genome shotgun sequence.
ACCESSION   DAOQGS010000200 DAOQGS010000000
VERSION     DAOQGS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5925_length_6447_cov_4.516114"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKZ52_08965"
     CDS             <1..570
                     /locus_tag="PKZ52_08965"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015003.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNG59945.1"
                     /translation="TVVGFLKEHGLDKDFKLNIETNHATLAQHTMDHELQVAADAGML
                     GSIDANRGDYQNAWDTDQFPNNINETVELMLVILRAGGLQGGGVNFDAKTRRNSTDLA
                     DMFYGHIGGMDTFARALLIADELLQKSPLEGLRKERYATFDSGRGAEFEQGKMSLADL
                     AAIGNSNGEPAQTSGKQELYELIINRYIR"
     gene            965..2467
                     /locus_tag="PKZ52_08970"
     CDS             965..2467
                     /locus_tag="PKZ52_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG59946.1"
                     /translation="MSTSSPAFVSATNQKLSVVEKIGYSLGDLSANLIFQTLVTFLAF
                     FYTDVFGIPASEATFIIGTVGLVGAFVFTPLMGILADRTNTRWGKFRPWILWTAIPFG
                     VLSILAFTTPDLTQDQKIVYAYVTYTLLLLIYAANNLPYSALSGVLTSSIEERNSVSA
                     YRFVAVMVAQLIIQVLLLPLALMLGDGDKAAGFHNTMMLFAGMGTLFFLISFATTKER
                     VLPIVESTNSVIDDLSDCAKNLPWIIMLIVTVLAFVSLALRGGITIYYFQNYLSAADL
                     ANFLESIGFNGFIAGLNAVFMGMGFSEFQWPKDPVISANSLFAACNIIMQIIGICCSK
                     SLADKFGKRNVYAFFLTIASSFILAFNFFSPSSIGTIFIAQILQGFFYGICVPILWAM
                     ISDVADYSEWKNNRRATAIIFSAMLIGLKAGLTIGGSLVAGLLAHYGYSATLTTQNAE
                     TVAGIKSIMSVYASLPFFLSVVCLFFYAINKKMELTLEADLNARRQAAGK"
     gene            2489..3505
                     /locus_tag="PKZ52_08975"
     CDS             2489..3505
                     /locus_tag="PKZ52_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNG59947.1"
                     /translation="MSEQNDAVDYQALKAKAISQPLVEHIYTADPSAHVFDGKIYIYP
                     SHDIEAGIEFNDNGDHFDMRDYHVLSLDTPESPAVDNGVALHVKDVPWAERQMWAPDA
                     ARKNGKYYFYFPAKRKDGIFQIGVAVGDSPVGPFIPEPEAIKGSYTIDPAVFEDNDGT
                     FYMYFGGIWGGQLQKYRNNTYNPANEEPADHEPALGPIVARLSDDMKEFAETPREIVI
                     LDEQGEPLTAGDHDRRFFEASWMHKYNGKYYFSYSTGNTHFLCYAIGDNPYGPFTYGG
                     KILTPVVGWTTHHSICEYQGQWYLFYHDSVLSGGVTHLRSVKMTPLHYDADGRIITID
                     PYRD"
     gene            3676..>6447
                     /locus_tag="PKZ52_08980"
     CDS             3676..>6447
                     /locus_tag="PKZ52_08980"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNG59948.1"
                     /translation="MTTDTTYQPAAIEQTWYQHWESQGYFAPSHQGDPYCIMIPPPNV
                     TGSLHMGHGFNNAVMDALIRLKRMQGHNTLWQVGTDHAGIATQMVVERQLAAKNISRH
                     DLGREAFLEKVWEWKAESGGAITQQLRRLGSSVDWSRERFTMDDGFYKAVQEVFIRLH
                     ADGLIYRGKRLVNWDPKLHTAISDLEVESSEEQGSLWHFRYPLSDGSGYLVVATTRPE
                     TMLGDTAVAVNPTDERYSHLIGKTIRLPLVGREIPIIADDYVDKEFGTGCVKITPAHD
                     FNDYEMGQRHQLEMINIFDKNAHLNDAVPEQYRGLERFAARKQVVADLDALGLLEKIE
                     PHQLKVPRGDRSGVVIEPYLTDQWYVRTAPLAAQAIAAVEDGHIQFVPKQYENMYFAW
                     MRDIQDWCISRQLWWGHRIPAWYDPEGNPYVGRDEASVRKAHNLPDELPLKQDEDVLD
                     TWFSSGLWTFGTLGWPEKTRELAAFHPTNVLVTGFDIIFFWVARMIMLTLHFQQEVPF
                     KTVYVHGLVRDGQGQKMSKSKGNVLDPIDLIDGIDIDTLVHKRTSGLMRPKDAEKIEK
                     ATRKEFPNGIAAYGTDALRFTFYSLASTGRDINFDVGRIEGYRNFCNKLWNAANYALM
                     NTQEQDCGQDNSTDYSLGLAERWIISQLQQAETQVLEHMGQLRLDLASQALYDFVWKD
                     YCDWYIELSKPVLWDDQAAADIKKGTRRTLIRVLEAILRLAHPFMPFITEEIWQKVKT
                     LAGKSGPSIMLAPYPVPDTSKINLQAVADVEWVKGVITAIRTIRGEMNVPPSKKIAVF
                     VSGGAAGTQQRVSDNNAFLKKLASLESIAYLSDNEEAPLSATALFGDVKILVPMAGII
                     DKQAELARLDKEIDKLKKEVERVQAKLSNPAFTDKAPADVVQKEQDKLTGYAQAIAQL
                     TEQQGKIAAL"
BASE COUNT         1613 a         1618 c         1526 g         1690 t
ORIGIN      
        1 accgtagttg gcttcctaaa agaacacggc ttggataaag atttcaaact caatattgaa
       61 accaaccacg caacccttgc gcaacacact atggatcacg agctgcaagt ggccgctgat
      121 gccggcatgt tgggcagcat cgacgccaac cgcggcgact accaaaacgc ttgggatacc
      181 gaccaattcc ccaacaacat caatgaaact gttgaattga tgttggttat tttgcgtgcc
      241 ggcggcctgc aaggtggcgg tgtgaacttc gacgcaaaaa ctcgccgcaa ctccactgat
      301 ttggctgata tgttctatgg ccacatcggc ggcatggata cttttgcccg cgcactgtta
      361 atcgctgatg agctgttgca aaaatcaccc ttggaaggcc tgcgcaaaga gcgttacgcg
      421 acctttgatt ctggcagagg cgctgaattt gagcagggta aaatgtcatt ggccgactta
      481 gctgcgattg gcaatagcaa tggtgaaccc gcacaaacta gcggtaaaca agaactgtac
      541 gaattgatta ttaaccgtta catccgttaa tcacaggtct ttaattcgca cccaaggatg
      601 ggtgtgtttg caacctatcc acaccccatg gacgcaattg gttgcaaaac ttgcgcaccc
      661 ttttcccatc ctatcccaca gtttttctct cattttttac tctcaactcg ctttacagcc
      721 gccctatttc agcgcgtgta cgcgcttggc atttctgcgt tattttttgt tcgattgatc
      781 gctttttagg ccttggatgc agtttttttt gaggcatttg tgtatattgc gcgcctttag
      841 tggcgatatt tagtgctcgc catccaccaa ctgtccgccc tttttacccc cagtgcttgt
      901 ggacggtttt tgcggtaagc gtagcctttt ggggaatggc tacacgaatg aagactgaat
      961 aataatgagt acatcctctc ctgcatttgt ctccgccacc aatcaaaaac tctcggtggt
     1021 agaaaaaatt ggttatagtc ttggcgattt gtcagcgaac ctaatctttc aaacactagt
     1081 aacctttctt gccttttttt ataccgatgt attcggtata ccggcctctg aagcaacttt
     1141 cattatcggc actgtaggtt tagtgggggc gtttgttttt acgcccttaa tgggaattct
     1201 cgccgaccgc accaataccc gctggggtaa atttcgcccc tggatactct ggacagctat
     1261 tccattcggt gttttatcga ttttggcatt caccacacct gatctcactc aggatcaaaa
     1321 aattgtctac gcctatgtca cctataccct gctgttatta atttacgccg ctaataattt
     1381 gccttactcg gccttaagcg gtgtactcac cagcagtatt gaagagcgca acagcgtttc
     1441 cgcctatcgc ttcgttgctg tgatggtggc ccagttgatt atccaagtgc ttctgttacc
     1501 cctagcgctc atgttaggtg atggggataa agccgccggt tttcacaata cgatgatgtt
     1561 gtttgccggt atgggcaccc tgtttttctt aatttccttt gccactacca aagagcgtgt
     1621 gttgccgatt gttgaatcca ccaatagtgt gattgacgat ctgagtgact gtgccaaaaa
     1681 tttaccctgg ataattatgc tcatagtcac ggtattagca tttgtgtcct tggcgctgcg
     1741 cggtggcatc accatttatt acttccagaa ttatttaagt gctgccgacc tggccaactt
     1801 tttagaaagc ataggtttta acggctttat cgctgggtta aacgccgtat ttatgggcat
     1861 gggctttagc gaattccaat ggccaaaaga cccagttatc tcagccaata gcctgttcgc
     1921 cgcctgtaac atcattatgc aaatcatagg catctgctgt tcaaaatctc tcgccgacaa
     1981 atttggcaag cgcaatgtat atgcgttctt tctaactatt gcctcaagtt ttattctagc
     2041 gtttaacttt ttctctccct ccagtatcgg caccatcttt attgcgcaaa ttttacaagg
     2101 ttttttctac ggtatttgtg tgcctatcct gtgggccatg atcagcgatg ttgccgacta
     2161 ctcggaatgg aaaaataacc gccgcgccac ggctattatt ttctccgcca tgttaatcgg
     2221 cttaaaagcg ggcctcacca ttggcggctc actggtggcc gggctgctgg ctcactacgg
     2281 ttattccgct acactcacaa cacaaaacgc agaaaccgta gccggtataa aatccattat
     2341 gagtgtctat gcgtccctgc ccttcttcct gtcagttgtg tgtttattct tctatgccat
     2401 taacaaaaaa atggagctta ccctagaagc cgatttaaac gcccgccgtc aggccgctgg
     2461 caaataattc aatccatcgg agctatttat gtcagagcaa aatgacgcag ttgattatca
     2521 agcattaaaa gctaaggcca tttcgcaacc cttagttgag catatttata ccgccgaccc
     2581 atctgcccac gtgtttgatg gcaagattta catctatccc tcccacgata ttgaagccgg
     2641 tatcgaattt aacgataacg gcgaccattt tgatatgcgt gattaccacg ttttatccct
     2701 ggacacgccc gaaagccctg cggtggataa cggtgttgca ttgcatgtaa aagacgtgcc
     2761 ctgggctgag cggcaaatgt gggcgccgga tgcggcccgt aaaaacggca agtattactt
     2821 ttattttcca gccaaacgca aagatggcat ttttcaaatt ggtgtggctg tgggggattc
     2881 accggttggg ccctttattc cagagcctga ggccatcaaa ggcagctaca ccattgaccc
     2941 tgctgtgttc gaagataacg acggcacctt ttatatgtat tttggtggca tttggggtgg
     3001 gcagctgcag aagtatcgca acaacactta taaccccgcc aacgaagaac ctgctgacca
     3061 tgaaccagcc ctggggccga tcgtggcgcg cttaagcgac gacatgaagg aatttgccga
     3121 aacacctagg gaaatcgtca ttctggatga acagggtgaa cccttaacag cgggtgatca
     3181 cgaccgccgc ttttttgaag cctcgtggat gcacaaatac aacggcaagt attatttttc
     3241 ctactccacc ggcaataccc actttttgtg ttatgccata ggtgataacc cctacgggcc
     3301 atttacctac ggcggtaaaa ttttaacccc cgtggtcggc tggactaccc accactctat
     3361 ttgtgaatat caaggccagt ggtatttgtt ttatcacgac tctgtactgt ccggcggcgt
     3421 tactcatttg cgctccgtga aaatgacgcc gctgcactat gatgctgacg gccgcattat
     3481 cactattgat ccctatcgcg attaattgct ggtacaactg ggcgaaggag tgccccattt
     3541 gaatcttaaa catttccccc ttcccgcacc gccctagagc ttccgtgaat ttcccgctcg
     3601 cgcaccaata aggggttctg ccaaattgtt ggccatgtat aatcaacggt ctttttagac
     3661 catttgattc gccctatgac cacagatacc acctaccaac ccgccgccat cgaacaaacc
     3721 tggtaccaac actgggaatc ccagggctat tttgccccca gccaccaagg cgatccctac
     3781 tgcatcatga ttccgccgcc caatgtcacc ggcagcctgc acatgggcca tggttttaac
     3841 aatgcggtaa tggatgcgtt aattcgctta aaacgcatgc agggccacaa tacactttgg
     3901 caggtgggca ccgatcacgc gggaatcgct acacaaatgg tggtagagcg gcagttggcc
     3961 gccaagaata tcagccgcca cgatttaggc cgtgaagcct ttttagaaaa agtctgggaa
     4021 tggaaagccg aatcgggtgg cgccattacc caacaattac gccgcctagg ctcctctgta
     4081 gattggagcc gggagcgctt caccatggac gatggttttt ataaggcggt gcaagaggta
     4141 tttattcgcc tacacgccga tggcttaatt tatcgcggca aacgcctagt caactgggac
     4201 cctaaattac acactgccat ttccgattta gaagtggaaa gcagcgagga gcagggcagc
     4261 ctgtggcatt ttcgctaccc tttaagtgat ggcagcggtt atttagtggt ggccaccacg
     4321 cggccggaaa ccatgctcgg cgataccgct gttgccgtta atccaaccga tgagcgctac
     4381 agccatttaa tcggcaaaac catcaggctg ccgttggtgg ggcgagaaat tcccattatt
     4441 gccgatgatt atgtggataa agaattcggc accggctgcg tgaaaattac ccccgcccat
     4501 gattttaacg actatgaaat gggccagcgg catcaattgg agatgattaa tatcttcgat
     4561 aaaaatgccc atcttaatga tgccgtgccg gagcaatatc gagggcttga gcgttttgct
     4621 gcgcgtaaac aagttgtcgc cgatttagac gccttaggcc tattagaaaa aatcgaaccc
     4681 caccagctaa aagtgccgcg cggggatcgc agcggcgtgg tcattgagcc ttacctcacc
     4741 gatcaatggt acgtgcgcac ggcgccattg gctgcgcaag ccatcgccgc cgttgaagac
     4801 ggccacattc aatttgtgcc gaaacagtat gaaaatatgt attttgcctg gatgcgggat
     4861 attcaagact ggtgtatttc ccggcaattg tggtggggcc atcgcattcc cgcctggtac
     4921 gatccggaag ggaatcccta cgtgggccgc gatgaagcga gtgtgcgcaa ggctcataat
     4981 ctgccggatg agctgccgct aaaacaggat gaagacgttt tagatacctg gttttcgtcc
     5041 ggcttatgga catttggcac cttaggatgg ccggaaaaaa cccgtgaatt agccgcattc
     5101 cacccaacca atgttttagt gacgggcttt gacattattt tcttctgggt ggcacgcatg
     5161 attatgctca ccctgcattt ccagcaggaa gtgccgttta aaactgttta tgtccacggc
     5221 ttggtgcgcg acggccaggg ccaaaaaatg agtaaatcca agggtaatgt tttagaccct
     5281 atcgatttaa tcgacggcat tgatattgac accctagtgc ataaacgcac cagcggatta
     5341 atgcggccca aggatgcgga gaaaattgaa aaagccaccc gtaaagaatt ccccaatggt
     5401 atcgccgctt atggcaccga tgcgctgcgt tttacttttt attctttagc cagcaccggg
     5461 cgggatatta attttgatgt ggggcgcatc gaaggctatc gcaatttctg caataaatta
     5521 tggaacgccg ccaactatgc attaatgaat acccaagagc aggactgcgg ccaggataac
     5581 agcactgatt acagccttgg cctggccgag cgctggatta tcagccaatt gcagcaggcg
     5641 gaaacgcaag tgttagaaca catggggcaa ctgcgcttgg atttggccag ccaagcgctg
     5701 tatgactttg tgtggaagga ttattgcgat tggtatattg aattatccaa acccgtgtta
     5761 tgggatgacc aggcggcagc ggatattaaa aaaggcacac ggcgcacact tattcgcgtg
     5821 ctcgaagcta ttttgcgcct agctcacccg tttatgccgt ttattaccga agaaatttgg
     5881 caaaaagtta aaaccctggc gggtaaatcc ggccccagta ttatgctggc cccctacccc
     5941 gtacctgata ccagtaaaat taacctgcag gcagtggcgg atgtggaatg ggtaaaaggt
     6001 gtgatcactg caattcgcac cattcgcggt gaaatgaatg taccacccag taaaaaaatc
     6061 gccgtttttg tcagcggtgg cgcagctggc acgcaacagc gtgtgagtga caataatgca
     6121 ttcttgaaaa aattagccag cctggaatcc atcgcctatt taagcgacaa cgaagaggca
     6181 cccttaagtg ctacagcctt atttggcgat gtaaaaatcc tggtgcctat ggcggggatt
     6241 attgataaac aggctgaact tgcacgcttg gacaaggaaa tcgataaatt gaaaaaagaa
     6301 gtcgagcgcg tgcaggctaa attaagcaac ccggcattta ccgataaagc ccctgccgat
     6361 gtagtgcaaa aagaacagga taaactcacc ggctacgccc aggctattgc tcaactcact
     6421 gagcaacagg gtaaaattgc cgcccta
//
LOCUS       DAOQGS010000201         6432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5943_length_6432_cov_4.618002, whole genome shotgun sequence.
ACCESSION   DAOQGS010000201 DAOQGS010000000
VERSION     DAOQGS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_6432_cov_4.618002"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..849
                     /locus_tag="PKZ52_08985"
     CDS             13..849
                     /locus_tag="PKZ52_08985"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNG59949.1"
                     /translation="MYSLRVLIDFLHARQIQTYMDCETAEGIAPGATQHVTRAQLAQL
                     CDLVIVVGGDGSVLSTGRSMRGTGVPLLGINRGRLGFLTDIAPDEIANRVGAVLDGEY
                     FDEERFLLECRLVRQGTIIATGTALNDMVLHPGQFIKMIEFKLYIGDRFVYQQRSDGL
                     IISTPTGSTAYALSGGGPIMHPSLDAMVLVPLNPHTLSSRPIVVAGNSQIRIVIGDQR
                     AVEPQLTCDGQTHITTQEGDEIIVHKKSDTLRLIHPLDYDFYTVCRSKLGWAVDTQGK
                     IE"
     gene            859..1821
                     /locus_tag="PKZ52_08990"
     CDS             859..1821
                     /locus_tag="PKZ52_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNG59950.1"
                     /translation="MNQLGYDIIGDVHGCAFTLVKLLEKLGYRDFNGVYQHPTRKAIF
                     VGDILDRGPHIREAITLVKAMVDAGVAECVMGNHEYNALGYHIWLPDQQRYLRDHTAR
                     NHRLLSATLEQYKKHPNEWEQVLNWIQQRPLFLNLPACRVVHACWDSDLIYTYECNYG
                     GNHVSREFLVESGEETSFAYLLMDRLTRGTDIALPPGIEVVGRDGFSRKQFRTKFWST
                     DPQTYADVVFQPDPLPAYLMNTPLSKSERKCLLHYSEQEVPVFVGHYWLTGVPAPLTP
                     NVACLDYSAVKYGRLVAYRFDGEAVLSADKFVSVVVDMKSDVGH"
     gene            2635..5391
                     /locus_tag="PKZ52_08995"
     CDS             2635..5391
                     /locus_tag="PKZ52_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNG59951.1"
                     /translation="MSNSFNVVLTGELLKGQSIMQAANSLSKLFKIAPIQAQQMLARA
                     PLVIKKNLEQDTAVRFVQAMYKSGFGAAVEEQRPTKTAFTHPFNSHYDDEDEEEAAVT
                     RITPVLVEQSRLEESLDTELTIPVAAVAPSVQPPVVAVTSQVQRLEPVALVAAASSAS
                     ELSPAPPEAPPIAAPEQTAVPEHFLAEEIDAATAPVADGEDQAAELSAVDATDDLVQD
                     NWEDEGIESIVVTHIEPLEYAAPQSEQVQEPQADEPRLDELAEETHGTAAIALPDPFA
                     KWDETSLQLEHSLADHPAEAVEQAYLVEEIATESVDPIAETQAALYADEGVAEPIPAA
                     EVFAPVEAEAHRSADLPADTGELSSHAADETAEVEIVAEPFSAIEGTFDADVFEPAPA
                     LAEVAVEAAEAADEAIPTAEVHLSEEQLGEVESDAEFVADIQVTDIQVAEVQWLEPVA
                     QWQTVAELAEDLTQEQGVAASYDTAEQPAPAEAMAEQGAWDGKTAGAPPADFTPHPAP
                     FAEIDEPLLEEPAQARWDEAVEISPALTERLAQIAEASKEITEASALNEADSEDAQLE
                     DNDTAEGVLLFEEADVTEISSASWDDWHASDAMTASGAAAFNPLLDFEQVVPDWQNSS
                     LELLFDTPQDSHQGGVTAANEQELAAFLDAPADYQDEPQAFAPEAVEDNTDLVGADEL
                     TPADALVPEYADAQEQSHAEASASNTGSAWAHEISVQANCGFLEVVIPKGKELHVAAA
                     ASVTMGLSLHEQLNAGSNVRAWLLAKGFVINTYEAIESAGNLGITAITHGMVCTIDAS
                     DFPVMVQQSAYLASTDDVQLNADQSRLNPVVSASKWIHCSGTGELWLNAQGLWEMVEV
                     DGCYRINSRYLMAYSKSLELKAPPLAGLKSYLTSGSGVECRLTGRGVVWVQTEASRAP
                     VIWQ"
     gene            5633..5902
                     /locus_tag="PKZ52_09000"
     CDS             5633..5902
                     /locus_tag="PKZ52_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNG59952.1"
                     /translation="MYQVLVDTDDFVVVDKAAGVPVHGPSPQPTLIEQLRADFENPAL
                     NLVHRLDNGTSGLVLIAKNPTANRLLAQLFAERAVCKRYLAIRAQ"
     gene            5928..6290
                     /locus_tag="PKZ52_09005"
     CDS             5928..6290
                     /locus_tag="PKZ52_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59953.1"
                     /translation="MVKVRDGNWMLKHSRDNPARTYFISAALKPNCRLFVLAPQTGKT
                     HQLRVALRSQQAAILGDERYGTVGADRLYLHSWQLRFHFQGQDHEFMAWPTRGELFQD
                     EGLRNLAMELAGRLPQGI"
BASE COUNT         1510 a         1568 c         1776 g         1578 t
ORIGIN      
        1 agtgaagcgg cgctgtactc gctgcgtgtg ctgattgatt ttttacacgc tcggcaaatt
       61 caaacctata tggattgtga aactgccgag gggattgcgc caggggccac ccaacacgtc
      121 acccgtgccc aactggcgca gctgtgtgat ctggtcattg tggtgggcgg cgatggcagt
      181 gtgttgagca ctggccgctc catgcgcggc accggtgtgc ccctgctggg gattaaccgc
      241 ggccgcttag ggtttttaac ggatatagcg cccgatgaaa tcgccaatcg cgtaggcgcc
      301 gttttagatg gtgaatattt tgatgaagag cgctttttat tggagtgccg cttggtgcgt
      361 caaggcacga ttatcgcaac cggtacagca ctgaatgaca tggtgttgca tccggggcaa
      421 tttatcaaaa tgattgagtt taaattgtat atcggcgatc ggtttgtgta tcaacagcgc
      481 tccgacggtt taattatttc cacccccaca ggctctaccg cctacgcctt aagcggtggc
      541 gggccgatta tgcacccctc tttagacgcc atggtcttag tgcccttaaa cccccacacc
      601 ctgagcagcc ggccgatagt ggtggcaggc aatagccaaa ttcgcatcgt cattggcgat
      661 cagcgcgcgg tggagccaca attaacctgc gatgggcaaa cccacatcac cacccaagag
      721 ggggatgaaa ttattgtgca caaaaagagc gacaccctgc ggttaattca tccgctggat
      781 tacgattttt acaccgtatg ccgcagtaaa ttgggttggg ctgtggatac acaagggaaa
      841 atagaatagg tgcggcaggt gaatcagttg ggttacgaca ttattggcga cgtgcacggc
      901 tgtgctttta cgctggtgaa attgctggag aaattaggct atcgggattt taatggggtt
      961 tatcagcacc cgacgcgcaa ggctattttt gtcggcgata ttttggatcg cgggccccat
     1021 attcgcgaag cgattaccct tgttaaggcc atggtagatg ccggtgtggc cgagtgtgtg
     1081 atgggtaatc atgagtacaa tgcgctgggc taccatattt ggctgccgga tcaacagcgt
     1141 tatttacgcg atcacacagc acgcaaccac cgcctattaa gtgccaccct agagcaatac
     1201 aaaaaacatc ctaacgaatg ggagcaggtt ttaaattgga ttcaacagcg ccccttattt
     1261 ttaaatttgc cggcctgccg cgtggtgcac gcctgttggg atagcgattt aatttatacc
     1321 tatgaatgca attacggcgg caatcatgtc agccgggaat ttttagtgga atccggcgaa
     1381 gaaacgagtt tcgcttattt attgatggat cgtttaaccc gcggcactga tatagccttg
     1441 ccgccgggca ttgaagtagt tgggcgcgac gggtttagtc gcaaacaatt tcgcaccaaa
     1501 ttttggtcta ctgatccgca gacctatgcc gatgtggtat ttcaaccgga ccctttgccc
     1561 gcctatttaa tgaatacccc cttaagcaaa tccgagcgca aatgtctttt gcattacagt
     1621 gagcaggaag tcccggtatt tgtcgggcac tattggctca ccggcgtacc cgccccatta
     1681 acaccgaacg tggcgtgttt ggattacagc gcagtgaaat atggccgttt agtggcttat
     1741 cgctttgatg gtgaagcggt tttgtcagcg gataaatttg tatcggtggt ggtggatatg
     1801 aaaagtgatg tggggcatta agccgaggtt ttatcgcggg caactattta ttaggggcgc
     1861 ctgctacaaa tattcgcttc tcactcaagg cataagtttt tgcgtccgca aacccttata
     1921 tataccgcct aacctcgttt attcggctat gtttgcccac ggcctcctgc tttaatttca
     1981 gtccgcgaga aatattagct ttctcacacc gggcaattcc tttggattga gaaatctctg
     2041 ctcaatacgc tttttgacac gatttaagcg cgcagattca agtattaggc accctcgcta
     2101 cgagttcaac tgcggttttt cggttaaaca gagttttcct ggttaacgtg atatctataa
     2161 tgagttgact catggggtgc gcgcagctgt tcatggcgca ggtgagaggt aggtttacct
     2221 ggttgtgtga tctgcgcccc ttcgccttga tctggctgcc tgttctctta cctagcgcac
     2281 tgggtacgat gtaattaatc ccagtcctgg gatgacaaat gcgtgccgga ttatcagtcg
     2341 aatgggcgtg atagattttt ctaggttagt aaaacgatca aatttcatgt tagataattt
     2401 ggcactttgg tctggggccg cgccagggaa cgagacactg tacaaaaaaa ccgcaatttc
     2461 aagccgcagg ctttttgtct gagcagcaag atcagttact attagtctcc ccgtacatta
     2521 ggtcagtaat cgctatggca ctcctctctt ggcaactgca tcagccctcg ctgagcaccc
     2581 ggcggcttta tcgcctgttt ttggtgttga gcgctttagt gatggaggtt ctccgtgtct
     2641 aattcattta acgttgtgtt aacaggtgaa ttgcttaaag ggcagtccat catgcaggcg
     2701 gcgaatagtt taagcaaact cttcaaaata gcgcccattc aagcacagca gatgctcgca
     2761 cgggcacctt tggtaatcaa aaaaaatctg gaacaagata ccgctgttcg atttgtccaa
     2821 gccatgtata aatcaggttt cggcgcggcg gtggaagaac aaaggcccac taaaaccgcc
     2881 ttcacacatc catttaattc ccattatgac gacgaggatg aagaagaggc cgctgtaaca
     2941 cgtattactc ccgtcttagt agaacagtcc cgcttagaag aaagcctgga tacagaatta
     3001 acgattcccg tggcggcagt tgcgccatca gtgcaacccc cagtggtagc cgtcacttca
     3061 caagtgcagc gtttagagcc agtggcgttg gtagccgctg cttcctcagc tagtgaatta
     3121 tcgcccgccc cgccagaagc acctccaata gcggcgcctg agcagaccgc agtcccagaa
     3181 catttcctgg ctgaagaaat tgatgccgcc actgcccccg ttgccgatgg cgaggatcag
     3241 gcggccgagc tctctgcagt tgatgcaaca gacgatctgg tgcaggacaa ctgggaggat
     3301 gaaggtattg agtcaattgt ggtaacacac attgagccgc ttgaatatgc cgcgccccag
     3361 tcagagcaag tacaagagcc tcaggcagac gaacctcggc ttgatgaact ggctgaagag
     3421 acccatggta ctgctgccat tgccttgccc gacccatttg ccaaatggga tgagactagt
     3481 cttcagttgg aacatagcct agcggatcat ccagctgaag ccgtcgagca ggcctactta
     3541 gttgaagaga tagcgaccga aagcgttgat ccgatcgctg agacccaggc tgcgttgtat
     3601 gcagatgaag gcgttgccga gccaattcct gccgccgagg tgtttgcccc tgtcgaggcc
     3661 gaggcgcacc gttctgccga tttgcccgca gataccggcg agctgtcatc acacgcagca
     3721 gacgaaacag ccgaagttga aattgtggca gaacccttca gcgcaattga gggaaccttc
     3781 gatgccgacg tgtttgagcc tgcgcctgcg cttgctgagg tggcggttga ggctgccgag
     3841 gctgcagacg aggcaatacc gacggctgaa gtgcacttga gtgaggagca actgggcgag
     3901 gtagaaagtg acgctgagtt tgttgccgat attcaggtta ctgatatcca ggttgctgag
     3961 gtgcaatggt tagaacccgt cgcccagtgg caaacggtag cggaattggc tgaggattta
     4021 acccaagagc agggcgtagc tgccagctac gataccgcag aacaaccggc acctgctgaa
     4081 gctatggctg agcagggcgc ttgggatggc aaaactgcag gtgcgccgcc cgcggatttt
     4141 acgccccacc ccgccccctt tgctgaaata gacgagccgc ttttagagga gcctgcgcag
     4201 gcgcgctggg atgaggccgt tgaaatatcc ccggccctga cggagcgctt ggcgcaaatt
     4261 gccgaggcca gcaaagagat cacagaagcc tccgcgctga acgaggccga tagcgaagat
     4321 gctcagttgg aagacaatga cacagccgaa ggcgtgttgt tgtttgagga agctgatgta
     4381 acagaaattt cctccgccag ttgggacgac tggcacgcta gtgatgccat gacggcatct
     4441 ggcgccgctg ccttcaaccc attattggat tttgagcagg tggtgccgga ttggcaaaac
     4501 agctccctgg agttgctgtt cgacacccct caagatagcc atcaaggtgg tgtgacggct
     4561 gcgaatgagc aggaattagc ggcttttttg gatgcacctg cggactatca agacgagcca
     4621 caggcattcg cgcctgaagc tgtggaggac aatacagatc tagtcggggc ggacgaattg
     4681 acgcctgccg atgctttagt gcccgagtat gcggatgccc aggagcagtc ccatgcagaa
     4741 gcgtccgctt cgaatacagg cagcgcctgg gcccacgaaa ttagcgtaca agcgaattgt
     4801 ggctttttag aggtggtgat tcccaaggga aaagagctgc atgtggcggc ggcggcctcg
     4861 gtaaccatgg ggctgagcct acacgagcag ctcaacgcgg gcagcaatgt gcgggcttgg
     4921 ttgctggcaa agggttttgt tatcaatacc tacgaggcga ttgagtcagc gggtaacttg
     4981 ggcatcaccg ccattaccca cggcatggtg tgcaccatag atgccagcga ttttccggtg
     5041 atggtacagc aaagtgccta cttggcatct accgatgatg tgcagctcaa tgccgaccaa
     5101 agccgcttaa atccggtggt aagtgccagt aagtggattc attgctcggg tactggtgag
     5161 ctgtggctga atgcacaggg cctgtgggaa atggttgagg tggatggctg ctaccgcatt
     5221 aattcccgct atttaatggc ctattcaaaa agcctggagt taaaagcacc acccttagcc
     5281 ggtttgaaat cctacctaac ctccggcagc ggcgtggaat gccgtttaac tgggcgcggt
     5341 gtagtctggg tgcaaactga ggcttcgcgg gcaccggtta tttggcagta aatctcaccc
     5401 attgattaaa cccgctgcgg cgggtttttt tatgtggttt ttaggtttaa agccattttg
     5461 cgggctggtg cctcaatgcc gcagccgctt tggggttgga aatttcacaa aatacctaaa
     5521 taagtacccg ccctaaaaac tagcgggttg cagcctgtgc ccgctatgac atgcgccctc
     5581 ggttgtcctg cagtgaaaat tgtttagaat cgccggattt tttgggtgaa ttatgtatca
     5641 ggtgctggtc gataccgatg attttgttgt ggtcgataaa gcagcgggtg taccggtaca
     5701 cgggccaagc ccgcagccaa ccctcattga gcagctgcgc gcggattttg aaaatccggc
     5761 acttaatctt gtccaccgct tagataacgg cacctccggt ctcgtgttaa ttgccaaaaa
     5821 tcccaccgcg aatcgcctgc tggcacaatt atttgctgag cgcgccgtgt gcaaacgcta
     5881 tttggccatt agagcccagt aaaaaacaag gctgggtgat tggggatatg gtgaaagtgc
     5941 gcgacggcaa ttggatgctt aaacacagcc gtgataatcc tgctagaacc tattttatca
     6001 gcgccgcctt aaaacccaat tgcaggttat ttgtgctcgc gccgcaaacc ggtaaaaccc
     6061 accaattgcg cgtagcgctg cgcagccaac aggcagctat tttgggggat gaacgctacg
     6121 gtactgtcgg cgcagatcgt ttgtatttac attcctggca actgcgattt cactttcaag
     6181 ggcaggatca tgagtttatg gcttggccta cacgtggcga attatttcag gatgaagggc
     6241 ttaggaattt ggctatggag ttggcggggc gcttacccca gggaatttag ttgcagaatc
     6301 attgattaaa tctgtttgcg gggatgttca ccgtgcgcat aaattagatt ttcttattat
     6361 gaatccggcc cggacattta aatggctctt cgtagaattg tgtaaaaccc ccgcgccaga
     6421 tggaaaaatg gc
//
LOCUS       DAOQGS010000202         6419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_5964_length_6419_cov_9.043055, whole genome shotgun sequence.
ACCESSION   DAOQGS010000202 DAOQGS010000000
VERSION     DAOQGS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_6419_cov_9.043055"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..333)
                     /locus_tag="PKZ52_09010"
     CDS             complement(55..333)
                     /locus_tag="PKZ52_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59954.1"
                     /translation="MSDLIEKIRVKLIEEGFDGLYMEGVFGGCACHVDDLAPCGECQK
                     ERGEDYINGCEPGHKFSNPDDPSLWVVKSKNIAPTAEEWIKFESGYFL"
     gene            complement(653..991)
                     /locus_tag="PKZ52_09015"
     CDS             complement(653..991)
                     /locus_tag="PKZ52_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59955.1"
                     /translation="MSIELFRPSNATHGEFFHEEFCYRCTKFPRSSDAKKQALEILKP
                     PFKFCCGYIFDSAEQTNMVADDGGSPDSLSRVRGWGRLGKLPNGAEVQDAIGEIIAEA
                     LNQYFERLKP"
     gene            complement(988..1476)
                     /locus_tag="PKZ52_09020"
     CDS             complement(988..1476)
                     /locus_tag="PKZ52_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59956.1"
                     /translation="MASVRKKFNPAAGKQRIAALRQKNRDERRAQKLEMADAEIRKML
                     KGVMLSYNSTWEQDIREGEEGRQVVHTTGLERLRDALKGDKEMTFGACIEIVIHWRVY
                     LTIYNVTQDGEEYVITYPVIDRRDSMINIITEIQGAIIPVELKKRNQAHVKDWGYWGE
                     IV"
     gene            complement(1549..1887)
                     /locus_tag="PKZ52_09025"
     CDS             complement(1549..1887)
                     /locus_tag="PKZ52_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59957.1"
                     /translation="MQPIHKPTEAARLKIEQDIKAFLKRKGKIDVEPIRVRKPGKYKN
                     FCIVSTKQEFHKAQELRREQNKKVYEVKWCQRELMYHIYDGKTKVSKDGYISADLART
                     EAIKLQEADQ"
     gene            complement(1901..2245)
                     /locus_tag="PKZ52_09030"
     CDS             complement(1901..2245)
                     /locus_tag="PKZ52_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59958.1"
                     /translation="MESITPMTVIFPAIVFAVAFFLGWLTGQLRDNAATQALMDKIEA
                     LKSELKHREWQLKNATEHEAFLDHENAVLNKELDRRQNIINAYKIGQEPKPDQPQMPD
                     EIPDELLTSPHL"
     gene            complement(2395..2877)
                     /locus_tag="PKZ52_09035"
     CDS             complement(2395..2877)
                     /locus_tag="PKZ52_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59959.1"
                     /translation="MSGTVLKLELDLSEMFHQSEEGGEYSIKEIFMDAVKSEVARSLR
                     EFVRSDIARTFSDFVSDELKSEIRAEMRIKLRNFMDTQELSEGYGKVTVSAYLERMFN
                     ELIQGSTIRNQVADIAKSWGAELKKQYDIAYANKLVQTLNDQGLLLPNVGQLLLDKSS
                     "
     gene            complement(2913..4547)
                     /locus_tag="PKZ52_09040"
     CDS             complement(2913..4547)
                     /locus_tag="PKZ52_09040"
                     /inference="COORDINATES: protein motif:HMM:NF021128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HNG59960.1"
                     /translation="MITLDLIQGSQEWRAKRQECYTASEASAMMGASKYQTRDQLLAA
                     KSAGIVEEIDSAKQRLFQRGHDAEAINRPAVEALIGQELYPVTGFSEIEGLPLLASFD
                     GITMDETIIFEHKLANQNLIASIKNGELEPHYYWQIEQQLLVSGAEKAIFVCGDETGI
                     AATIEYFPVPGRKEQLIAGWHQFSKDLETFVPKAKAQKAIAQPVEALPAINYTIDLSN
                     GISINSNLEVFKLAAQALVERSKELLVTDQDFENAKARVKQCESAEKNITSLIQRVLG
                     ELGDVNKFKEDMESIKEWIRRSRLNQDKQIKERLTTRKAEIIAAGRKAAEEYTAWVNM
                     NLGRVRLPAIGCDLEGAVYKKSSFASMESAVNDAVANFKIAADKWSAHLTQSLNIFDQ
                     AAEGYAELFRDLQQLCEKDTDSLKAIVKVRIDEFKAKEQERAEAEAKRLAAQKAAQET
                     AAQAAELAAKVAAQAAAEQTPVAVISSVQTAAPANDERPIPSHLSIGGGGGSYRTKPI
                     PKSLPTSEQVINAVAIHFNMQSVDAERLLIDLFSNR"
     gene            complement(4534..5430)
                     /locus_tag="PKZ52_09045"
     CDS             complement(4534..5430)
                     /locus_tag="PKZ52_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HNG59961.1"
                     /translation="MNNLTVSTDQSAVTTNSIIIQSEMFDRVMSLASLMASGSATIPK
                     HLQKNQADCAAIIMQSMQWGMNPFAVAQKTHITQGGSLGYEAQLVNAVIMANGPLDEH
                     PKYEFFGEWDRILGRVKEMQTEGKGKWYAKDWKPEDENGLGVICHYKIRGETGWRSYE
                     LLLKQCWPRFSTQWATDPQQQICYAAIKKVARRDFPGVILGVYTRDELEEAPGEIDIT
                     PPKQSEPQPEQPKELPFYPQEQFDKNLPLWQQLITTGKKTAKAVIDSIKSRAQLTEDQ
                     IATIETIESIEGQAEEVTDDNA"
     gene            complement(5561..6055)
                     /locus_tag="PKZ52_09050"
     CDS             complement(5561..6055)
                     /locus_tag="PKZ52_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59962.1"
                     /translation="METRPSYGKAIVNALRDSGVKVDVHFNYRALPEPKQDIACRELI
                     LTGNENIACECIPGVTEYGDHLAHLYWAFPMFYAVILRKRIYDQAKADKSQELEQALA
                     EYSCALETEATLRAGLTKGRLLMLLVSDPQEFLFQVNLAVIKYLNSFIESEIEDVLRE
                     VVCH"
     gene            complement(6060..>6419)
                     /locus_tag="PKZ52_09055"
     CDS             complement(6060..>6419)
                     /locus_tag="PKZ52_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59963.1"
                     /translation="DLRGTQITALPENLSVGGYLDLRGTQITALPENLSVGGSLDLHE
                     KTQNAAFKKNCGRQNRTIFAAWVNGEIQIGAGCFLGSIATFCEAVDESYKGEAADKYK
                     ADAQDCVDRLVAILNKE"
BASE COUNT         1458 a         1620 c         1410 g         1931 t
ORIGIN      
        1 attacgcaaa atcataaaca gtagtagcaa tattttttgc aataagctca tcgctcataa
       61 aaaataaccg ctctcaaact taatccactc ttctgccgta ggtgcaatat ttttagattt
      121 aacaacccac aagctaggat catccgggtt ggaaaactta tgccccggct cacaaccatt
      181 tatataatcc tcgccgcgct ccttttggca ttcgccgcac ggcgccagat catcaacatg
      241 gcaggcgcaa ccaccaaaaa cgccctccat gtaaagcccg tcaaatcctt cctcaattaa
      301 tttaacccgg atcttctcaa ttaaatcact cataaatact cccgcgctga cctggttttc
      361 gactgatacg tcggcgcaat ttttctagca aggccaatta ggtttggcgc catttcaaag
      421 agtctggcgc ttagtttttt tcaattcgcg cattcatcgc atttctccag cgagtgccgc
      481 caaaaatgtc aatacgccag cagcaaaaaa atacgaccca tggcgatctg ggttgccatt
      541 tagaattata aaaatggcgc ctacgacgca gacgcacggg aaaaggtact tcatattttc
      601 acctctgtgc acgaacctct gttagctccg gtattgggtg attgcattgg gttcatggct
      661 tcagccgctc aaaatattga tttaatgctt cggcaattat ttcacctatg gcgtcttgaa
      721 cttcagcgcc atttggaagc ttgcctagcc ttccccatcc tctaaccctg gacaatgaat
      781 ctgggctgcc tccatcatcg gcgaccatgt ttgtttgttc ggcgctgtca aaaatgtagc
      841 cacaacagaa tttaaacgga ggctttagaa tttccagcgc ttgttttttt gcatcgcttg
      901 agcgcgggaa ctttgtgcag cgataacaaa actcttcatg aaaaaattca ccatgagtgg
      961 cattgcttgg cctaaaaagc tctatgctca cactatctct ccccaatatc cccaatcttt
     1021 gacgtgcgcc tggttgcgct ttttaagctc aaccggaatt atcgcgccct gaatttcggt
     1081 aatgatattt atcattgaat cgcgcctatc aattaccgga taagtaatca cgtactcttc
     1141 accgtcctgg gttacgttgt agattgttaa atacactcgc cagtgaatta caatctcaat
     1201 gcaggccccg aaggtcattt ctttatcgcc ctttagtgca tcccttaatc gttcaagccc
     1261 ggtcgtgtga accacttgcc ttccttcctc gccctccctt atgtcctgct cccatgtgct
     1321 gttgtaggag agcattacgc ccttgagcat tttgcgaatt tcggcgtctg ccatttctag
     1381 cttttgcgct ctccgttcgt ctcggttttt ctggcgtaaa gctgcaatcc tttgtttgcc
     1441 agcggcaggg ttaaactttt tgcgaacact agccacggaa aacaaccgcc gcgcagataa
     1501 agaaaattac actggccgta aaaaatatta ccctggcttg tggtgtgttc attggtcggc
     1561 ctcttgcagt ttgattgcct ccgttcgcgc caagtcggca gaaatataac catccttgct
     1621 aaccttggtt ttgccgtcgt aaatgtgata catcaattca cgctggcacc acttaacttc
     1681 gtaaaccttt ttgttttgct cccttcgcaa ttcctgtgct ttgtgaaatt cctgcttagt
     1741 ggaaacgatg caaaaattct tatacttgcc tggcttccta acgcggatcg gttcaacgtc
     1801 gatctttcct ttgcgcttca aaaatgcctt aatatcctgc tcaattttga gcctggcggc
     1861 ttcggttggc ttatgaatcg gctgcacact aaaactccgg tcaaaggtga ggggaggtta
     1921 atagctcgtc aggtatttcg tccggcattt gcggctgatc tggttttggt tcctggccga
     1981 ttttataggc gtttataatg ttctggcggc ggtctagctc cttgttcaaa acggcgtttt
     2041 cgtgatcaag gaatgcctcg tgctcggtag cgtttttgag ctgccattct cggtgcttta
     2101 attccgactt taacgcctcg attttatcca taagcgcttg agtggcggca ttgtcgcgaa
     2161 gttggccagt tagccaacca agaaaaaaag cgactgcaaa aacgatggcg ggaaaaatta
     2221 ctgtcattgg tgttattgat tccataaaac tctccgaatt aaataaaacc ctggattacg
     2281 cgccagggcg cggaagtgtc accgggcgga tatgacgcac ttgaggcaac ccaagccact
     2341 tctgcggcag tttctgttta agtcgcacac accgctaacc gacttttccc cgcgctaaga
     2401 cgatttatca agtaatagct ggccaacatt tggcagcagc aatccctgat cgttgagggt
     2461 ttgcaccagc ttattggcgt aagcaatgtc gtattgcttt ttaagctctg cgccccagct
     2521 tttggcaatg tcggcaacct gattgcgaat ggttgaccct tgtattagtt cattaaacat
     2581 tcgctcaaga tacgcagata cggtaacttt tccatagccc tcgcttagct cctgagtgtc
     2641 cataaaattg cgcaacttaa tccgcatttc tgcgcgaatc tcgcttttta attcgtcaga
     2701 aacaaagtca gagaaagtgc gagcaatatc ggatcgaaca aattcgcgca aacttctagc
     2761 gacttcggac tttactgcat ccatgaaaat ttcctttatg ctgtactcgc caccctcttc
     2821 tgactggtga aacatttcgg atagatcgag ttcaagtttt aatactgtgc cgctcatata
     2881 aagctcctaa ttggtagtta ataaaatcaa ggttagcgat tgctgaataa atcaattaac
     2941 aagcgctctg catccactga ctgcatgttg aaatgaattg ccacggcgtt aataacctgc
     3001 tcggacgtgg gtaggctttt tggaataggc tttgttcggt atgagccacc tccaccgcca
     3061 atacttaggt gcgaaggaat tggccgctcg tcgtttgctg gtgccgctgt ctgcacactg
     3121 gaaattactg ctactggcgt ttgctctgcg gctgcttgtg ccgccacctt tgctgctaac
     3181 tctgcagctt gcgcggcagt ttcctgcgcg gctttttgtg cagccaggcg ctttgcttcc
     3241 gcttcggctc gctcttgctc cttggcttta aattcgtcaa ttcgcacctt cacaattgcc
     3301 ttgagcgagt cggtatcttt ttcgcaaagc tgctgcaggt cgcggaataa ttcggcatac
     3361 ccctctgcgg cctggtcaaa aatattaaga ctctgggtga ggtgagcgct ccatttatcc
     3421 gcagcaattt tgaagttggc cacggcgtca ttaactgcgg attccatgct tgcaaagctg
     3481 ctttttttgt aaaccgcgcc ctcaagatcg caaccgattg cgggcagccg aacacggccc
     3541 aggttcatgt ttacccatgc ggtatattct tcggccgcct tgcgtccggc cgcgataatt
     3601 tccgcctttc tggtagttag acgctcttta atttgcttat cctggtttaa tcgtgaccgg
     3661 cgaatccact ccttaatact ctccatgtcc tccttgaatt tattcacatc gccaagctcg
     3721 cctaaaacgc gctgaattaa agaggtgata tttttctctg ctgattcgca ctgcttaacc
     3781 cgcgccttgg cgttttcgaa atcctgatct gtaaccaata attcctttga tcgctccacc
     3841 agcgcttgcg ctgccagctt gaacacttcc aaattgctgt taatgctaat gccgttagat
     3901 aaatcaattg tgtaattgat agccgggagc gcttcaacgg gttgtgcaat tgctttttgt
     3961 gcctttgcct ttggtacaaa tgtttcaagg tctttggaga attggtgcca tcctgcaatc
     4021 aactgctctt ttcggcctgg caccgggaag tattcgattg ttgcggcaat cccagtttca
     4081 tcgccacata caaaaattgc cttttcggcg ccagacacta atagctgctg ctcgatttgc
     4141 cagtaatagt gcggctcaag ttcgccattt ttaattgagg cgattagatt ttgattggcc
     4201 agtttgtgct caaaaataat agtttcgtcc atggttatgc cgtcgaagct agccaaaagc
     4261 ggcaggccct cgatttcgga aaaccctgtt accgggtaaa gttcttggcc gattaatgcc
     4321 tcaactgctg ggcggttgat ggcctcggca tcatgcccgc gctgaaataa gcgttgcttg
     4381 gcgctgtcga tttcttccac tatcccggct gatttagcgg ccaataactg gtcgcgagtt
     4441 tggtatttgc tggcgcccat catggcgctt gcctcggatg cggtgtagca ctcctggcgc
     4501 ttggctcgcc attcttgtga gccctggatt aaatcaagcg ttatcatcgg taacttcctc
     4561 tgcttggccc tcgatggact caatggtttc tattgttgca atttgatctt cggttaattg
     4621 cgccctgctt tttattgaat caatgaccgc ttttgctgtt ttcttgccag tggttattaa
     4681 ttgttgccac aaaggcaggt ttttatcgaa ttgctcttgc gggtagaaag gcaattcttt
     4741 aggttgttct ggttgcggct ctgattgttt tggcggtgta atatcaattt cgcccggcgc
     4801 ttcctctagt tcgtcccgcg tataaactcc aaggatcacg cctggaaaat cgcggcgggc
     4861 aaccttttta atagctgcat aacaaatttg ctgttgcgga tctgtggccc actgagtaga
     4921 aaaacgcggc cagcattgct ttagcaataa ttcatagctg cgccaccctg tttcgcctct
     4981 tattttgtag tggcaaataa cgcccaggcc gttttcatct tccggcttcc aatccttcgc
     5041 gtaccatttg ccttttcctt ctgtttgcat ctccttaacg cgccctaaaa ttcgatccca
     5101 ctcaccgaaa aattcatatt ttgggtgctc atcaagcggg ccgtttgcca taattaccgc
     5161 attaacaagt tgggcctcat aaccaaggct tccgccttgg gtgatatgag ttttttgcgc
     5221 taccgcgaaa ggattcatgc cccactgcat tgattgcata ataatggcgg cgcaatctgc
     5281 ttggtttttc tgtaggtgct tggggatagt tgctgagccg ctagccatta atgaagccaa
     5341 gctcatcact cgatcaaaca tttcagattg aatgattatg ctgtttgttg ttaccgcgct
     5401 ttgatcggta gaaaccgtta gattgttcac tttgttgccc cgtccaataa tttaactaat
     5461 cccattttcg cccctttgtt atcggggcca atggagtgga atttggcgct accgattttt
     5521 atgcagccta atttgctgcc gtagcgtgat tttttaagag ttagtgacat acaacctcgc
     5581 gcaatacatc ttcaatctcg ctctcgataa atgaatttag gtatttaata accgctaaat
     5641 tcacctgaaa taagaactct tgcggatcgc tcaccagaag cattaaaagg cggccctttg
     5701 ttaatccggc gcgcagggtt gcttcggttt caagcgcgca cgaatattca gcaagtgctt
     5761 gttcaagctc ctgtgattta tcggcttttg cttggtcgta gattcttttg cgcagaatta
     5821 ctgcgtagaa catggggaat gcccagtaca aatgggctag gtgatcgccg tactcggtaa
     5881 cgcctggaat acattcgcag gcaatatttt cattgccagt taaaattaat tcacggcagg
     5941 caatgtcttg cttgggttcc ggtaatgcgc ggtaattaaa atgcacatcg actttaacgc
     6001 cgctatcgcg caatgcgtta acgatggctt tgccgtaact cggtctggtt tccatttgcc
     6061 tactccttgt taagaattgc gactaagcgg tctacgcaat cttgcgcatc ggctttatat
     6121 ttatccgccg cctcgccttt gtaactttca tctaccgcct cacagaacgt ggctatggaa
     6181 ccaagaaagc agcctgcacc tatctgtatt tctccattaa cccaggccgc aaatatggtt
     6241 ctgttttggc gaccgcagtt tttcttaaat gccgcgtttt gggttttttc atgcagatcg
     6301 agcgagccgc ccaccgataa attctcaggc aatgcggtaa tttgtgtgcc gcgcagatcg
     6361 agatagccgc ccaccgataa attctcaggc aatgcggtaa tttgtgtgcc gcgcagatc
//
LOCUS       DAOQGS010000203         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6092_length_6331_cov_3.985660, whole genome shotgun sequence.
ACCESSION   DAOQGS010000203 DAOQGS010000000
VERSION     DAOQGS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6092_length_6331_cov_3.985660"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1565)
                     /locus_tag="PKZ52_09060"
     CDS             complement(12..1565)
                     /locus_tag="PKZ52_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59964.1"
                     /translation="MRQQVWASIIGRFALVISAVYLYGCAASQQVKPAPLTATVRDEM
                     VAPAGDELNLPWQATEGASAENWQAQQRNFLTLLADDANHVDAHSQLAELYFKQYIIA
                     KNADFKRWALYHAQKALATKPQDLTLLAAYYRLLYTQVRMEGEKTQLARLQTFYKGLP
                     SDARNSFFPPSLAFYLFLLEQDKNNRDNSRRAELKAVLQQALKEQPRNGLIHVQLSRY
                     YFDARQIDVGFAILHQALHLEPENPQVVLALAEAYRTRAQRTECVYDHLDDIKRSTQF
                     YKQLLTQPASNDDTRTPPTHMPQVHWGLMINYAHLGLAPLSIREGDQAMTADASALNK
                     WIFATYLTYQNQPVRAEALFATAKQQLPIIPTRAAVEHYVLQRQWQKAAQAFADYIAD
                     NEQPGVTDLLLASMIQAEIQDKSVTVASLWRTPKKAVFYNSFDEALAKFWRGDLTEAA
                     FAEQIKSPCQQAEYEFYVGYLHLLNNRVGKAKEAFELASKHPQPMNFEVQMAARFLQG
                     LLNNPKNRS"
     gene            complement(1562..3769)
                     /locus_tag="PKZ52_09065"
     CDS             complement(1562..3769)
                     /locus_tag="PKZ52_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNG59965.1"
                     /translation="MYRLAQFFVPLRPLVWLILVMLATPRSALASSIPFDQLPAPQYA
                     GKAAAEILIKRTRIELDEGLQATSHAYAAIYLHSDEAVRDYSQMSIGFNSFYEAVELE
                     FARVKTPDGKVVTLAADAVQVQSPSDENFYQDSRELTFSLPNLRPGATIEFQYTRREI
                     KKIIPNGWFDSFWFNWWEERAANQGMRVDPVVSASLDIFAPERLKLYVKPSAALKAEF
                     WQKQQGALTQWHWQQNQLPEIILQSGMPRSVNLFPMLQVTTLPNWNAVALWADGLFAP
                     HMVMDASITGIAQTLQKSAKTPQDKAKNLFGFMQDKIRYVFAHVGRGGYEPHTTTEIV
                     KNAYGDCKDQTMLAVALARSMGLTAYPALVATRSQGLAQLDIPAVNFDHMIVYLPKQT
                     GLDETWLDTTAESSLFPGFSTGIEGQQALIIKPDTQTLSVIPSRSAAQHKAQLTLRFK
                     TPTQEGIRADFKLDLAGVFEESLRATWLYAPEKQKTLTDMLGNIYNAGELQNLTVTNA
                     DNPFAPLSISGEFFFKNAWQGEGKPLTYGFGVGQLLNVFSDFVRMHKPAERKQALEND
                     PGYSLSAQVVFASPGVNYQPLTQSRGSNYHNPWFSLTQTADEEKNAVAVKVDWVLNKY
                     TVSLADYGKFYAAIQSVLDAAVWQVTFVPQEASAVNSTASGNKQAQLLSSVQRLIDAG
                     NFAEALIEAQKATRKFPNSGEAYYLLGLAQGYADQGEAAQQSFSKARELGFTP"
     gene            complement(3952..4953)
                     /locus_tag="PKZ52_09070"
     CDS             complement(3952..4953)
                     /locus_tag="PKZ52_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNG59966.1"
                     /translation="MQKKLRWGILSTAKIARTKVIPALFNSAHNEVVAIASRTLASAQ
                     TCAQELAIPTAYSSYEELLADPNVDAIYNPLPNDLHVPWSIAALKAGKHVLCEKPIGL
                     DTADTEQLIAASRAQPQLKVMEAFMYRFHPQWQAVKQWLAEGLIGEVKALQAYFTYNN
                     HEPDNIRNNPAAGGGALMDIGCYGISVARWVFDAEPVQLVAQLDLHPQYQVDQLALLN
                     VQFPKGGMASVLCGTKMESGQGVYISGSLGSITLERPFYCVGDEPNVVLVRRDTVVTR
                     HEFPGMDHYQFMVDAFAESCFKDLAVPTPLSDALANMQVIDAAFSSARSGLWQALPN"
     gene            complement(5189..>6331)
                     /gene="prfC"
                     /locus_tag="PKZ52_09075"
     CDS             complement(5189..>6331)
                     /gene="prfC"
                     /locus_tag="PKZ52_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNG59967.1"
                     /translation="RDPVEVLDEIETVLKIAAAPMTWPIGMGKEFKGVYNLYTDTFHL
                     YTQGQGHTIPDDIQIKGINSPEARKLLGAYVDDVQAELELVRGATHLFNLEDYLAGKL
                     TPVFFGTALGNFGVREMLDGFVEWAPPPQARASDVRPVQPHEPAFSGFVFKIQANMDP
                     KHRDRIAFMRVVSGSYTQGMKMQHVRLKKDVRISDAVTFLAGDRNTVEEAIAGDIIGL
                     HNHGTIQIGDAFTEGEALKFTGIPHFAPEMFRRIRLKDPLKAKALQKGLQQLSEEGST
                     QVFFPLNNNDIVVGAVGVLQFEVVAYRLKDEYKVEAIYEPVNVTTARWVECTDGKKFE
                     EFKRKCAENLALDGGGHLTYLAPTRVNLSLSQERHPEVVFRATREH"
BASE COUNT         1555 a         1680 c         1542 g         1554 t
ORIGIN      
        1 catagtagag ttcaactgcg gtttttagga ttattcaata acccctgtaa aaaacgcgct
       61 gccatttgca cttcaaaatt catcggctgc ggatgcttac tggccaactc aaaagcttct
      121 ttagctttgc ccacgcggtt atttaataga tgtaaatagc ccacataaaa ttcatactca
      181 gcctgttggc agggggattt tatctgctcg gcaaatgccg cctcggttaa atctccgcgc
      241 caaaacttcg ccaaggcctc atcaaagctg ttgtaaaaaa ccgccttttt aggggtgcgc
      301 cagaggctcg ccacagtcac agatttatct tgtatttcag cctgaatcat actggccaac
      361 aataaatcgg taacaccggg ctgctcgtta tcggcgatgt aatcggcaaa tgcttgcgcc
      421 gctttttgcc actgcctctg caacacataa tgctccaccg ccgcccgggt ggggattata
      481 ggcagctgct gcttagcggt tgcaaatagt gcctcagccc gtacgggttg attttgatag
      541 gttaaatagg tggcaaaaat ccacttattg agggcgcttg catccgcagt catcgcctga
      601 tccccctcgc ggatactcaa gggcgcaagc cctaaatggg cataattaat catcaacccc
      661 cagtgcactt ggggcatatg ggttggcggg gtgcgcgtgt catcattcga cgcaggctgg
      721 gttaataact gtttgtaaaa ttgtgtggag cgtttgatat cgtccagatg atcgtagaca
      781 cattccgtgc gctgggcgcg cgtgcgatag gcctccgcca aggccaacac cacctgaggg
      841 ttttcaggct ctaagtgcag ggcctgatgc aagattgcga accctacatc tatctgtcgc
      901 gcatcaaaat aataacggct gagttggaca tggattaagc cattgcgcgg ctgctctttc
      961 aaagcctgct gcagaacagc cttcaattca gcgcggcggg aattatcgcg gttattttta
     1021 tcctgctcca agaggaataa ataaaacgcc aaactgggcg ggaaaaaact attgcgcgca
     1081 tcgctgggca accccttata aaaggtttgc aaccgcgcca attgtgtttt ctcaccttcc
     1141 atacgcacct gggtatacaa caggcggtaa taggctgcta aaagagttaa atcctgcggc
     1201 ttggtggcta gggctttttg cgcgtggtag agcgcccagc gtttaaaatc ggcattttta
     1261 gcgattatat attgtttaaa atacagctct gcaagctggc tgtgcgcgtc aacgtgattg
     1321 gcatcatccg ctaacagcgt taaaaaattg cgctgctggg cctgccaatt ttcagcactg
     1381 gcgccttcag tagcttgcca gggcaagttt aattcatccc cagcgggggc caccatttca
     1441 tccctgactg ttgcagtcag gggagccggt ttgacctgct gagaagctgc gcagccatat
     1501 aaatatacgg cactaataac aagggcaaaa cggccgataa ttgatgccca gacttgttgc
     1561 ctcacggcgt aaaacctaat tcgcgggctt tgctaaatga ctgctgggcg gcctcgccct
     1621 gatccgcata accttgcgcc aaacccaata aataataggc ctccccagag ttggggaatt
     1681 tacgggtggc tttttgcgcc tcaatcagcg cctcggcaaa attacctgca tcaattaaac
     1741 gctgcacact gctaagcagc tgcgcctgtt tgtttccact ggcggtggaa tttaccgccg
     1801 aggcttcctg aggcacgaat gtcacctgcc aaaccgcagc atccaacact gattgaatcg
     1861 cggcgtaaaa tttgccgtag tccgcaaggc taaccgtgta tttattcaaa acccaatcca
     1921 ctttaaccgc caccgcattt ttttcttcat ccgccgtttg tgtgaggcta aaccaaggat
     1981 tgtgataatt gctgccacgg gattgagtca gcggctgata attcaccccg ggcgatgcaa
     2041 acaccacctg tgccgacagg ctatagcctg ggtcattctc tagggcctgt ttgcgctcgg
     2101 caggtttatg catgcgcaca aaatcgctaa atacatttaa taactgaccg acaccaaacc
     2161 cataggtaag cggtttgccc tccccctgcc aggcattttt aaagaaaaac tcgccggaaa
     2221 tggatagcgg tgcgaaggga ttatcggcgt tagtaacggt tagattttgc aattcaccgg
     2281 cgttgtaaat attgcccaac atgtcggtaa gggttttttg tttctcaggt gcgtacaacc
     2341 aggttgcccg caggctctct tcaaaaaccc cagctaaatc caatttaaaa tcggcgcgga
     2401 tgccctcttg agtaggggtt ttaaaacgca gggtcagttg ggctttatgt tgcgccgctg
     2461 agcgcgatgg aatgaccgac agcgtttgcg tatcgggttt gataattaac gcctgctggc
     2521 cttcgatgcc ggtgctaaac cccggaaaca aactgctctc ggcggtggtg tccagccagg
     2581 tttcgtccaa cccagtttgt ttgggcaaat aaacaatcat atgatcgaaa ttcaccgctg
     2641 gaatatccaa ttgagccaaa ccctggctgc gggtagccac caacgcgggg taagcagtta
     2701 aacccatact gcgcgccaag gccactgcca acatggtttg gtctttacaa tcgccatagg
     2761 catttttaac aatttccgtg gtggtgtgcg gttcatatcc gccgcgcccc acatgggcaa
     2821 atacataacg aattttatcc tgcataaaac caaataaatt tttagcttta tcctgcggcg
     2881 ttttggcgct tttttgcagg gtttgcgcta taccggtaat actggcatcc atcaccatat
     2941 gcggcgcaaa taatccatcc gcccagagcg ccaccgcatt ccagttgggc aaagtagtca
     3001 cctgcagcat gggaaataaa ttaacgctgc ggggcatgcc cgattgcaaa ataatctccg
     3061 gcaattgatt ttgctgccag tgccattggg ttaatgcgcc ttgttgcttc tgccaaaatt
     3121 cagcttttaa cgccgcgctc ggttttacgt acagcttgag gcgctccggc gcaaaaatat
     3181 ccaagcttgc actcaccact gggtctaccc gcatgccttg gttggcggcg cgctcttccc
     3241 accagttaaa ccaaaaacta tcaaaccaac cattgggaat tatttttttt atctcgcgcc
     3301 gagtatattg aaattcgatg gtggcgccgg ggcgtaaatt gggcagggaa aaggtaagct
     3361 ctcggctatc ttgataaaaa ttttcatccg atgggctttg cacctgtacg gcatccgcag
     3421 ccaaggttac caccttgccg tctggggttt ttacccgtgc aaattccagc tctaccgcct
     3481 catagaaact attaaagcca atgctcattt ggctgtaatc gcgcaccgct tcatcgctgt
     3541 gtaaataaat cgccgcgtag gcgtgggatg tggcctgcaa accctcgtca agctcaatgc
     3601 gcgtgcgctt aattaaaatc tccgcagccg ctttacctgc atattgcggc gcaggcaact
     3661 gatcaaaggg gattgatgat gcaagcgctg agcgcggcgt ggcgagcata accagaataa
     3721 gccacaccaa aggccgcaac ggcacaaaaa attgagctaa tcgatacaaa gttgggtcca
     3781 cacaaggcga aaaataaata ctaagcgggg cgcagaagtg ctgcgataca gaactacccc
     3841 accgcagccc cagggcggca aggcctaata aagaatagtc ggtgcggcca tcctggccct
     3901 aatacttatc aagaaaatta atgcttaatg cccacgcggc atttaatacc tttaattggg
     3961 caatgcctgc caaagtccgc tgcgggcact gctaaacgcc gcatcaatta cctgcatatt
     4021 ggccagcgcg tcgcttaaag gtgtaggtac ggctaaatcc ttaaaacagc tttcggcaaa
     4081 agcatccacc ataaactggt agtgatccat accggggaat tcatggcggg taaccactgt
     4141 atcccggcgc accagcacaa cattaggttc atcgcccacg caataaaatg gccgctctag
     4201 cgtgatggag cccaggctgc ctgaaatata aaccccctgc cccgattcca ttttagtgcc
     4261 gcacagcacg ctggccatgc cgcccttagg aaactgcaca ttcaacagcg ccagctgatc
     4321 cacctgatac tgcgggtgca gatccaattg cgccaccaat tgcacaggtt cagcatcaaa
     4381 cacccagcgc gccaccgaaa ttccataaca gccgatatcc atcagggcgc cgccaccggc
     4441 ggctgggttg ttgcgaatat tatccggctc gtggttgttg taggtgaaat aggcctgcaa
     4501 ggctttaact tcgccaatga ggccctctgc cagccactgt tttaccgcct gccactgggg
     4561 atgaaagcga tacataaagg cttccatcac ttttaactgc ggctgtgcgc ggctggccgc
     4621 tattaattgt tcagtatcag cagtatccag gccgatgggt ttttcacaca gcacatgttt
     4681 gcccgccttt aacgcggcaa tgctccaggg cacgtgtaaa tcgttgggca gcgggttata
     4741 gatggcatcc acattggggt ccgccagtaa ttcttcataa ctgctgtagg cggtggggat
     4801 ggccaattcc tgggcgcatg tctgggcgga ggcaagggtt cgtgaggcga tggccaccac
     4861 ttcattatgg gcactgttaa acagcgccgg aataactttg gtgcgggcga ttttagcggt
     4921 gcttaaaatt ccccaacgta gttttttttg catactcaat cctggcctta agaataaaaa
     4981 cgcgttttca acattattaa cccggcaaca ttaagtgccg ggttaatatt ggcggcatgg
     5041 atgccgctcc ggcggtgcaa accgccgcaa gctgcactaa aacaaggaaa tccacgcatt
     5101 tccgccagtt ttagtgcagc gaaggcaaaa tagatcagga cgatctattt tgccgccggg
     5161 ttaatagcat cgtcctattg cgaatagatt aatgctcacg ggtagcgcgg aacaccactt
     5221 ctgggtggcg ctcttgggat aaggataaat tcacccgcgt gggcgccaaa taggtcaaat
     5281 ggccaccgcc gtctaaggcc aggttttcgg cgcatttgcg tttaaattct tcaaattttt
     5341 tgccgtcagt acattccacc cagcgggcag tagtgacatt aaccggctcg taaatagctt
     5401 ccactttgta ttcatctttt aaacggtagg ccactacctc aaactgcaac acacccaccg
     5461 ctcccaccac aatatcgtta ttgttcagcg ggaaaaatac ttgggttgaa ccctcttctg
     5521 ataactgttg caggcctttt tgcagcgcct tggcttttag gggatccttt aagcgaatgc
     5581 ggcgaaacat ctccggcgca aaatggggaa tgccggtgaa tttcagtgct tcgccttcgg
     5641 taaacgcatc gccaatttga atggtgccgt ggttgtgcaa accgataata tcgcccgcaa
     5701 tggcctcttc caccgtattg cgatcaccgg ctaaaaaggt cactgcatcg ctgatgcgca
     5761 cgtctttttt caagcgcaca tgctgcattt tcatgccttg ggtataactg ccagatacca
     5821 cccgcataaa ggcgatgcgg tcgcggtgtt tcgggtccat attggcttga attttaaata
     5881 caaacccgga aaaggcaggc tcatgcggct gcaccgggcg cacatcactg gcccgcgcct
     5941 gcggcggcgg cgcccattcc acaaagccgt cgagcatttc ccgcacgcca aaattaccca
     6001 gcgcagtacc aaaaaatacc ggcgtgagtt tgcccgctaa ataatcctct aaattaaata
     6061 agtgcgtggc gccgcgcacc aattcaagtt ccgcttgcac atcatccacg taagcgccca
     6121 acagtttgcg cgcctcaggg ctattaatgc ctttaatttg aatatcgtcg ggtatggtgt
     6181 ggccctgccc ctgggtgtac aaatgaaatg tatcggtata gaggttgtac acgcctttaa
     6241 attctttgcc catgccgatg ggccaagtca tcggcgcggc ggcgattttg agcaccgtct
     6301 caatttcatc caatacttcc accgggtcgc g
//
LOCUS       DAOQGS010000204         6325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6102_length_6325_cov_4.486922, whole genome shotgun sequence.
ACCESSION   DAOQGS010000204 DAOQGS010000000
VERSION     DAOQGS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6102_length_6325_cov_4.486922"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..550
                     /gene="dtd"
                     /locus_tag="PKZ52_09080"
     CDS             110..550
                     /gene="dtd"
                     /locus_tag="PKZ52_09080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNG59968.1"
                     /translation="MRVLIQRVSSASVSVAGSEIGRINQGLLALVGIEPTDTAQTLTK
                     MANKLLAYRVFNDAEGKMNLNVQEVHGGLLLVSQFTLAADTDKGLRPGFSSAAPPAQA
                     ELLFNEFVTLVQSRWAATQTGKFGADMQVALINDGPVTFLLQMK"
     gene            560..2395
                     /locus_tag="PKZ52_09085"
     CDS             560..2395
                     /locus_tag="PKZ52_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG59969.1"
                     /translation="MFRLFENLIDPFKSVPITEPPQGLWAFCAHYSKGIWPILIAMAI
                     TTAAVSAIEVSLFHFLGTLVDKLNQHTPQTLWQQAGHELSLMALLILIGLPIAVYLRG
                     LLVHQSLLGNYPMLIRWRIHQYLLGQSVSFYQDEFAGRIATKLMQTALAVRETILKIL
                     DVFLYVVVFFSGILAMTFALDWRLSLPFLAWLIAYIGCLAFFIPRLTQAASIQADARS
                     EMTGRIVDTYTNMPTVKLFSHHRREAEYALDGMQQFLTTVHPQMRLVTLFSTSVWIIN
                     ALLIFSTSALSIFLWQQQALSTGAIAAAIGVVLRLYGMSQWIMWEVSGLFENIGTVYD
                     GISSIARPQQLKDAPHAQRLSLTRGAITFNDVCFDYGKSNNVIDHLNLHIQPGEKVGF
                     VGRSGAGKSTLVNLLLRFYDVKSGAILIDGQNIKEVSQESLRANIGMVTQDTSLLHRS
                     VRENIIYGKPDASDAELIHAAQQARAEDFIVELVDGKGRTGFDAHVGERGVKLSGGQR
                     QRLAIARVLLKNAPILILDEATSALDSEVEAAIQENLMLLMEGKTVLAIAHRLSTIAA
                     MDRLIVLDNGKIIEQGSHDELIQRGGLYAHLWAHQSGGFLAEDIQ"
     gene            complement(2525..3517)
                     /locus_tag="PKZ52_09090"
     CDS             complement(2525..3517)
                     /locus_tag="PKZ52_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNG59970.1"
                     /translation="MGLKQKVVGITLAWLSCAMAYGQGYVVTDNGALAVKGTQIINSK
                     GKPIALAGPSFFWSNNGWGAEGFFNAKTVAWAKNNWQATIVRAVVGVEGPGGYLESPD
                     ANKRRAYDVIDAAIAEDMYVLVDWHDHHGEQHTAEAVQFFVEVAKKYGAYPNVIYEIY
                     NEPKEVSWVKQVKPYAQTLIKAIRAEDPDNLIAVGTPRWSQDVDEVAKAPLTGVSNIV
                     YSLHFYAGTHHQALRDKAQKALDKGIALMVTEWGTVNADGKGAANARETQLWMEFLRK
                     HTLSHCNWALSAKNEGASIFNTDVKPADNYTDAQLTPSGLLAREWVRSWRELAR"
     gene            complement(4434..5339)
                     /locus_tag="PKZ52_09095"
     CDS             complement(4434..5339)
                     /locus_tag="PKZ52_09095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNG59971.1"
                     /translation="MNTYIALAPLSGFSLWAFYVAKTPSRAWRLLFIIVLAGYIFWLR
                     FRMQDLIIPVLVAAGCLLVVCLAEFTRLVSRGVLQGHGRFSKYLSKIPSRYLLVITAI
                     LWSAGILLIFAVGLHVLPGGESWQLSPPVRLGIAQVDWIWRVKLFNALLGIGLIILFY
                     KPKSITLQGVATLVAVCTTVFLLALIVGAWQNTWRWDPKIPSVWPWCCLLMLSFTAIP
                     EEAFFRYCIQAPLYQCVGGLSLFITALLFGFAHFSGGFIYAAVAALAGLGYAYIWHRF
                     QDVLLCSICHWLVNMLHFSLLTYPY"
     gene            complement(5759..5849)
                     /locus_tag="PKZ52_09100"
     tRNA            complement(5759..5849)
                     /locus_tag="PKZ52_09100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5813..5815),aa:Ser,seq:gct)
     gene            complement(6075..6269)
                     /gene="csrA"
                     /locus_tag="PKZ52_09105"
     CDS             complement(6075..6269)
                     /gene="csrA"
                     /locus_tag="PKZ52_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNG59972.1"
                     /translation="MLILTRRIGETLMVGDEVTVTVLGVKGNQVRIGVNAPKEIAVHR
                     EEIYQRIQREKQDQTDPGNR"
BASE COUNT         1747 a         1631 c         1380 g         1567 t
ORIGIN      
        1 tagagcaatg caggagcaat tgccgagttt acacgcagta aatgagcatt ttttggcaaa
       61 ttcaacgacg ccagagcgcg taaggtagtt tttcagaggt tccttaacta tgcgggtgtt
      121 aattcaacgg gtgtccagcg catcggtgtc ggttgcaggg agcgaaattg gccgcatcaa
      181 ccaaggccta ttggccttgg tgggcattga acctactgac actgcgcaaa cccttaccaa
      241 aatggccaac aaattattgg cctatcgggt atttaatgat gccgagggaa aaatgaatct
      301 aaacgtacag gaagtacacg gcggccttct gctggtatca cagtttaccc tcgccgccga
      361 taccgacaaa ggcctgcggc caggattttc cagcgccgcc ccaccggccc aggccgagct
      421 tttattcaat gaatttgtaa ctctggtgca aagtcgttgg gccgcgacgc aaaccgggaa
      481 attcggcgct gatatgcagg ttgcattaat taatgatggg cctgttacct ttctcttaca
      541 gatgaaataa ccctcctcta tgtttcgatt attcgaaaat cttatcgacc cttttaaatc
      601 tgtgcccatc actgaaccgc cccaagggct gtgggcattt tgcgcccatt actccaaagg
      661 catatggccc attcttatcg ccatggccat caccaccgca gccgtctccg ctattgaggt
      721 gagcctattt cattttttag gcacattggt ggataaactc aatcaacaca caccgcaaac
      781 cctctggcaa caggcaggcc acgaattaag ccttatggca ctattaattc ttataggctt
      841 accgattgcg gtgtatctgc gcggcttatt ggtccatcaa agcctcttgg gcaactaccc
      901 catgttaatc cgctggcgca ttcaccagta tttactgggg cagagtgtga gtttttacca
      961 ggatgaattt gccgggcgca tcgcgactaa attaatgcaa accgctttgg cggtgcggga
     1021 aaccatccta aaaatattag atgtgttttt atatgtcgta gtatttttca gcggcattct
     1081 ggccatgacc ttcgcgctgg attggcgcct gagcctaccc tttttagcct ggttaattgc
     1141 ctatatcggc tgcttggctt tttttattcc caggcttacc caggctgcca gtattcaggc
     1201 tgatgcccgc agcgaaatga ccgggcgcat agtggatacc tacaccaata tgcccacggt
     1261 aaaattattt tctcaccacc gccgggaagc cgaatacgcc ctggacggta tgcaacaatt
     1321 tttaactact gtacatccac aaatgcgcct ggtgacttta tttagcacca gcgtatggat
     1381 tattaatgcc ctacttattt tttcaacttc ggccctctcc atattcctgt ggcaacaaca
     1441 ggctctcagt accggcgcca ttgccgccgc cataggggtt gtattgcggc tgtacggcat
     1501 gagccaatgg attatgtggg aagtctccgg cctgtttgaa aatatcggca cggtatacga
     1561 cggcatcagc agtatcgccc gcccgcaaca attaaaagat gctccccatg cacagcgcct
     1621 gagcttaacc cgaggcgcca ttacctttaa cgacgtttgt tttgattacg gtaaatccaa
     1681 caatgtaatt gaccatttaa acctgcacat tcagccgggt gaaaaagtgg gttttgtcgg
     1741 ccgctccggc gctggcaaat caaccttggt gaatttgtta ttgcgctttt acgacgtaaa
     1801 atccggcgcc atattaattg atgggcaaaa tattaaagag gtcagccaag aaagcctgcg
     1861 cgccaacatc ggcatggtta cacaagacac ctcgttgcta caccgctctg tgcgggaaaa
     1921 tattatctac ggcaaacccg atgcaagcga cgcagaatta attcacgccg cccaacaggc
     1981 acgggcagaa gactttattg tggaattagt cgatggcaag ggccgtacag gttttgatgc
     2041 ccatgtaggt gagcgcggcg ttaagctctc cggcggccag cgccagcgtc tagccatagc
     2101 acgggtgctg ctaaaaaatg caccgatttt aattctcgac gaagccacct cagccctcga
     2161 cagcgaagta gaagcggcga tacaagaaaa tttaatgcta ttgatggagg gcaaaaccgt
     2221 gctcgccata gctcataggc tatcgaccat tgccgctatg gatcgtttaa ttgtgcttga
     2281 taatggcaag ataatcgaac aaggcagcca cgacgaactc attcaacgcg gcggcctata
     2341 cgcccatttg tgggcccatc agtcaggtgg ttttttggca gaagatattc aataacccac
     2401 tggtgaatgt tttttaaacc taaaaaacgc agttgaactc tactgcgttt tttttaggtt
     2461 aaaagacacg agtttgtccc aacacgctgc aatgcgggct tttcccgcat ggataagcct
     2521 ttatttaccg cgccaactcg cgccatgagc gcacccattc acgggccaaa aggccagagg
     2581 gtgttaattg agcgtcggta taattatctg ccggtttaac atcggtatta aaaatagacg
     2641 caccttcgtt tttagctgaa agcgcccaat tgcaatggct caatgtgtgt ttgcgcaaaa
     2701 actccatcca cagctgagtt tcgcgcgcat tggctgcgcc tttgccgtcc gcattcaccg
     2761 tgccccactc agtcaccatc agtgcaatgc ccttatccaa tgccttttgc gccttgtccc
     2821 gcagggcttg gtggtgtgta ccggcataaa aatgcaaact gtacacaata ttactaaccc
     2881 ccgttaaagg cgccttagcc acctcatcaa catcttgcga ccagcgcggc gtacccactg
     2941 caattaaatt atccgggtct tcggcgcgga tagcttttat taacgtttgt gcatagggtt
     3001 ttacctgttt tacccagctc acctctttag gctcattgta aatttcataa attacatttg
     3061 ggtaagcgcc atattttttg gccacctcaa caaagaattg aaccgcctcg gccgtgtgct
     3121 gctcgccatg gtgatcgtgc caatccacta atacatacat atcttcagcg atggcagcgt
     3181 caattacatc gtaggcgcgc cgtttatttg catccggaga ctccaaatag cctccagggc
     3241 cctccacgcc caccacggcg cgcacaatag tggcctgcca attatttttg gcccaagcca
     3301 ccgtttttgc attaaaaaaa ccttcagcgc cccaaccgtt attactccaa aaaaaactcg
     3361 gcccagccag ggcaatcggc ttgcctttag aattaattat ctgcgtgcct ttaacggcaa
     3421 gagccccgtt gtcggtaaca acgtagccct gcccataagc cattgcgcag ctcaaccacg
     3481 ccagagtaat acccacaacc ttttgtttta atcccatgac tgcctcatat tcggttttag
     3541 ttgtcgtatt tattaacccg gcggaaaaat agatcgtcct gatctatttt tcctacgctg
     3601 cgctaaaact tgcaaaaata cgtggatttt cttgttttag cgcagctcgc ggcgacttgc
     3661 gccgcctgtg cggcatccgc cgccagtatt aacccagcac taaatgccgc cgggttaaaa
     3721 taaaccccta ctcaattaga gtttccccag gcgcactggg catattcagc gccaacgcgg
     3781 attgctgacg gcgatggctc ttaatacact aattactatg aggctaccat cctaaacgct
     3841 attcgccccc atcactatcg ccggtaatgt ttaagggcgc ctccggacat tcaattttcg
     3901 tgatgctcgc taggtatttt ctcgcggaag gagggagtct atgccgtaca aatgaccgac
     3961 tgagcacgaa aacaacgagg tgagggcgca aaaaatggat ttccggagtt tcccttaagc
     4021 taagttagct cgtgcaacgt ctaggcaacc atgagtctag cagctgatac gccctagagg
     4081 ataatccaag cacttaattt tggatgttcg cacacaggct gaagccgtgc ttattgtgcc
     4141 caattacact ttgtgcccgg caactgatat ccgcgcttac aaacattttc agagcctcgc
     4201 aaagccattt ttaatggaca tcacattaat ctccatccga ttaatatgtc caagccgata
     4261 aaaagtaaaa caattatcaa agattgtcgg ctcccaccct gcaataaacc tcgctgatca
     4321 gtacaacccc tatttaaaat gcgccgcgta cctctgataa acggcactta ttaacccggc
     4381 agattcaact ccgaagtgca tcacagccgc cgggttagta aagacactat gaatcagtag
     4441 ggatatgtca gtaaagaaaa atgcaacatg ttgaccagcc aatggcaaat actacacagc
     4501 agcacgtctt gaaatctgtg ccaaatatag gcataaccta ggcccgccag ggcggcaact
     4561 gctgcataaa taaatcctcc actaaaatga gcgaaaccaa ataataacgc tgttataaac
     4621 aaacttagcc caccgacgca ctgatacaag ggagcctgaa tgcaataacg aaaaaatgcc
     4681 tcttcaggaa ttgctgtaaa actaagcatt aacaggcaac accacggcca tacgctgggt
     4741 attttggggt cccaacgcca ggtgttttgc caagctccaa cgatcaaggc caatagaaat
     4801 accgtggtgc ataccgcgac aagcgtggca acgccctgta gggttatgga cttgggcttg
     4861 taaaataaaa taattaaacc aatgcctagg agcgcattaa ataatttgac tcgccaaatc
     4921 caatcgacct gtgcaatccc taaacgcact ggcggcgata attgccagga ttcaccgccg
     4981 ggtaacacat gcaaacccac cgcaaatatc agtaagattc cggcgctcca gagtattgct
     5041 gtaatcacca gcaaatagcg actcggtatc ttgctaaggt attttgaaaa ccgcccgtgg
     5101 ccttgcagaa cgcctcttga gaccaagcgg gtaaactctg caaggcacac caccaataaa
     5161 cagccagcgg cgaccaacac agggatgatg aggtcttgca tcctaaacct aagccaaaag
     5221 atataaccag ccaagacaat aatgaatagt agcctccatg cacggctagg tgttttcgcc
     5281 acataaaacg cccacagcga aaatccactc aagggagcaa gtgcaatgta tgtattcata
     5341 acgccttagg tttctgctga ccaaatgctt attcagcggg caaatatatc tgtaacggat
     5401 gctttgaata aacatgaatt cgtgaatatt gtgcaaacac acatttacct cccaacagcg
     5461 ctcgcgcccc tgaacttgat cagaggttcc ttacattgaa atatattgag attgagaaag
     5521 acgccaagta tcgatgacgt caagtatcga taacacactg ctgacggtga aaacgattat
     5581 cgcgcactct aaaaagaact cacaccaaga ataaatgcac tcagcagaac aggaatttta
     5641 aatacaaaat cctatggtaa tagcgggaaa ttgacagcca cctaagcgga aaagcaatac
     5701 gtaaagatta ataaaagagg ggtaaataaa agacggatag agacggagat ataaagaatg
     5761 gcggagaggg agggattcga accctcgata cccttttgag gtatactccc ttagcagggg
     5821 agcgccttcg gcctctcggc cacctctccg gaaatacagt caaaatactg taattaatta
     5881 acgctacact tatttaaagc gtcgcacctc aaataaaagc gttccgcctc ggtttgcaag
     5941 ttaccctgca tccttcggcg ggcggcataa taccaacata tttccagaag ttaaagttta
     6001 ataagcaaat atttttaaaa atattcaatt attcccataa cttacggacg agaaccccag
     6061 cggattcaaa aagactagcg gttgcccggg tcagtttgat cttgtttttc gcgttgaata
     6121 cgttgataga tttcttcacg gtgaacggcg atttctttcg gtgcattcac accaatacga
     6181 acctggtttc ctttcacacc caaaacggtc acggtaactt catcaccaac catcagggtt
     6241 tcaccaatac gacgggttaa aattaacata aaaatctcct actgcttcct gtgagaacga
     6301 cagcatttat tgcctggctg ctgac
//
LOCUS       DAOQGS010000205         6319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6110_length_6319_cov_4.100415, whole genome shotgun sequence.
ACCESSION   DAOQGS010000205 DAOQGS010000000
VERSION     DAOQGS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6110_length_6319_cov_4.100415"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..5017)
                     /locus_tag="PKZ52_09110"
     CDS             complement(29..5017)
                     /locus_tag="PKZ52_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNG59973.1"
                     /translation="MTVQSRTQKAVVLAIALVALGLMFSGLVALQFHLANERQLQRHL
                     QEDAQKEVAKVELRLASYRQALYNARSFVLAHNGHPSPQAFAQYVSFINLAEEFPGMR
                     GIGVIDRIKPADEQAFIERMHQEGLDTFNLRFIKPHDGDRYVIRLIEPIINNRQARGL
                     DTASEELRRDAAERAYVRNTPQLTGPITLMQAQDKVTQAFLFFLPVFADWQIPSNEAD
                     RARLHIGWVYEPLVMQEILRGAHFNDDLFHYVISDITTDNHQRIYACEDYIELKSIAA
                     VVLQSEIFGRQWQFAITPTEKYLRQLDLTEPKLVFISGMLATLLLAFLAAILHINLAQ
                     RRHTQAQNARLAALIEGTADAIIEVNLSGTIEQWNRGAEAMFGFSSLEAVGQPLTQLI
                     VPEAQRPQAENELMAAIEQRTKTDALAQRCTGQGALLDVLVYTAPLLGEQNKVIGAAT
                     IIRDMSKLRAAERELKELNAHLETRVALRTAQVVQARDELESLVNALPYAVLAVSADG
                     RIYKANQKSRERFASSGHNLLGERLAEVVPAEYYAQIAPWLFYLPEGQVNSGQYSYLQ
                     EQGLAHYQWILIPAISQHTYKGFYFVEQDVSILKNNKIKLAATLAEQEALLKSINTQF
                     LLSITDQGGMIQSVNEPFCQFSGYRPEELIGKSHSIMKSGLHSKDFWGQVWQQILSGE
                     PWRGEVCNRHRNGDFYWFDTVIAPIRGADGKIERFMALRSDISARKDAEAARNRISQL
                     LTSVLNAASEVGIIATDLNGLITVFNRGAERLLGYSAEEMIDKLTPAAFHVPDEVNAR
                     SEELSLQYAEPIAGFDTFIYVPRKMEAETRQWTYVRKDKTEIPVMLTVTSIRNAEGVI
                     EGYLGIAMDISEQLAAAAQVAAVTEQLLQAASVANLGIWTWVLSSNELHWNEQMFALY
                     QQDPALQLSGQLNYQHWAERLVPEDLILAQNALQAAINGEAEYDLILRLNLPDGALRY
                     IKAQGRVSRDKHGAPVSITGINIDITELYLHEEELRKARDLAQEASRAKSQFLANISH
                     EIRTPMNAVLGMLQLLQTTVLTERQQEYSHNAIGAARSLLGLLNDVLDFSKIEAGKLQ
                     LDAHEFSLEHLLHDVAIVLTGIQGNKRQVEVLFDIGRDIPRYLIADKLRLQQILINLT
                     SNALKFTEQGCVKLSIKLLKEDAQQVRLEVRVEDTGIGIAPEHQQKIFDGFTQAEAST
                     TRRFGGTGLGLVICQRLLTLMQCQLNLSSTQGVGSQFWFEVQLPIGTTHAAQGLDVFY
                     QQVLLIEDNTYARALVAEQLTAWSGQVQAVANVDEAAPFISQLENNAFDLVVVDWRLA
                     QENGLAAVEALAQQVSAPLPPVVFMATTDDKELLMQRCAQHSFAPAGIMLKPIIAWHW
                     QHLWAAMASATPVKTIAAQPAPNNYVLQGLTLLVVEDNELNRQVAYGLLTHLGAQVDL
                     ANSGLEAVDKLIGQQAGYDLVFMDMQMPDMDGLQATRRLRQEARLQALPIVAMTANVS
                     PADVAACLDAGMNDHVGKPLDIEHILAVMGRYVALADTAGPATKPPLAAQSQVEAITE
                     SWPNILARFGQNTKLLSRVRDRFAAELTEQLTQLRAALDTQEKTDQARVFHSIKGVAA
                     TVGACKLAKWCAEQEKIIKSGTACDAHQLAQLTQLCDEATAAIAQPFTEHLP"
     gene            5580..>6319
                     /locus_tag="PKZ52_09115"
     CDS             5580..>6319
                     /locus_tag="PKZ52_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HNG59974.1"
                     /translation="MTLPPISRPRLTIYCAVFITLFANWALWQKLLQLLSPLTLDEIP
                     FCLSALVLIFAIAHGVCHLLALGRLQKIWLIIALVCSAAASYYQVQYHVLYDKTMIQN
                     ILATDYREAYGLFNLKMVLWIALFGLLPATIISLWPIKPAPIKKQALHYGGGFLLSVV
                     LIAAVAGIFYKDYASLFRNNRDLKHLIIPSSFVYSLYGFVADKTKTTGEPVQVIGSDA
                     RLGPLWQAANKPVVFVLVVGETARRANFS"
BASE COUNT         1546 a         1725 c         1430 g         1618 t
ORIGIN      
        1 gcgagtaagg taatttttca gaggttccct agggtaaatg ctcggtaaat ggctgtgcta
       61 tagcggcagt cgcctcatca cacagctgtg ttaattgcgc tagctgatga gcgtcacagg
      121 ctgtaccact tttaataatt ttttcctgct cggcacacca ttttgccaat ttacacgcgc
      181 cgactgtggc cgccacacct ttaatactgt gaaatactct cgcctggtcg gttttttcct
      241 gagtatccaa cgccgcgcgc aattgtgtta attgctcggt taattctgcg gcaaaacgat
      301 cgcgcacgcg gctgagtaat tttgtatttt ggccaaaccg cgcgagaata ttcggccagg
      361 attcggtaat ggcctccacc tgcgattgcg ccgcgagcgg cggttttgtt gcagggcctg
      421 cggtgtcggc cagggccaca tagcggccca taaccgccag aatatgctca atatccagcg
      481 gtttgcccac atgatcattc atacccgcat ccaaacaggc agcgacatca gccgggctta
      541 cattcgctgt catagctaca ataggcaagg cctgtaagcg cgcctcttgg cgcaaccggc
      601 gtgtagcctg caaaccatcc atgtcgggca tttgcatatc cataaatacc aaatcatacc
      661 cagcctgttg cccaattaat ttatccaccg cctctaatcc gctgttggct aaatccacct
      721 gcgcacctaa gtgcgttaat aaaccgtagg ccacttggcg atttaattca ttgtcctcca
      781 ccactaatag agttaaaccc tgcaatacat aattattagg cgcaggctgt gccgcaatgg
      841 ttttcaccgg tgtcgcactg gccattgcgg cccataaatg ttgccagtgc caggcaataa
      901 tgggttttaa cataatcccg gcgggcgcaa atgagtgttg ggcgcagcgc tgcattaata
      961 gttctttatc gtccgtagtc gccataaata ctaccggcgg taagggtgcc gatacctgtt
     1021 gcgctaaggc ctctacagcg gctaaaccat tttcttgtgc gaggcgccaa tccactacca
     1081 caaggtcaaa ggcgttattt tccaattgcg aaataaatgg cgcggcctca tccacatttg
     1141 ccactgcctg cacctgccca gaccaagccg tcaattgctc ggccactaag gcgcgggcgt
     1201 aagtattatc ctcaattaat aatacctgct gataaaacac atcaaggccc tgcgccgcgt
     1261 gtgtagtgcc aataggcagt tgcacctcaa accaaaattg gctgcccact ccttgtgtac
     1321 tggaaagatt taattggcac tgcatgagtg ttaacaggcg ctggcaaatc accaacccca
     1381 gccccgtacc gccaaaccgg cgggtggtgg aagcctctgc ctgggtaaaa ccatcaaaga
     1441 ttttttgttg gtgctcaggc gcgataccta tgcctgtatc ttccacccgc acctctaagc
     1501 gcacctgctg ggcatcttcc ttgagcaatt taatactcag ttttacacag ccctgctcgg
     1561 taaatttcag tgcgttgctg gttaaattaa ttaaaatttg ctgcaggcgc agcttatccg
     1621 caattaaata ccgggggata tcccggccta tatcaaacaa aacttccact tggcgtttat
     1681 tgccctgaat accggtgagc acaatagcca catcatgcaa taaatgctca agggaaaatt
     1741 catgggcatc cagctgtaat ttacccgcct caattttaga aaaatccaac acatcattta
     1801 ataatcccag cagcgagcgg gcggccccta tggcattgtg gctgtattcc tgttgccgct
     1861 cggttaacac cgtggtttgc agcagttgca acatacccaa caccgcattc atgggcgtgc
     1921 gaatttcgtg gctgatatta gccagaaact gtgatttggc ccggctcgcc tcctgcgcca
     1981 aatcccgcgc cttgcgcagc tcttcctcgt gcaaatacaa ctccgtaata tcgatattaa
     2041 ttcccgtaat ggataccggc gcgccatgtt tgtcgcggct tacacggccc tgggctttaa
     2101 tataacgcag cgcaccatca ggcaaattta agcgtaaaat taaatcgtat tcagcctcgc
     2161 cgttaatagc cgcctgcagc gcattttgtg cgaggattaa atcctccggc accaaacgct
     2221 cagcccagtg ttggtagttt aattgcccag acagctgcaa agccgggtcc tgctggtaca
     2281 gtgcaaacat ttgttcattc caatgcagtt cattgctgct cagtacccaa gtccaaatac
     2341 ctaaattagc cacggaggcg gcttgtaata attgctcggt aactgccgcc acctgcgccg
     2401 ccgctgccaa ctgctcgctg atatccatgg caatacctaa ataaccttca ataacgccct
     2461 cggcattgcg aatagaggtc accgttaaca taaccgggat ttcggtttta tccttgcgca
     2521 cataggtcca ctgccgcgtc tccgcttcca ttttgcgtgg tacataaata aaagtatcaa
     2581 agcctgctat gggttcggcg tactgcaagg ataattcctc gctgcgcgca tttacttcgt
     2641 ccggcacatg aaaagccgca ggggttaatt tatcgatcat ctcctcggcg ctatagccta
     2701 acaaacgctc cgcaccgcga ttaaacaccg taattaagcc gtttagatcg gtggcaataa
     2761 tgccaacttc actcgcagca tttaaaacgc tggttaacaa ttgtgagatg cgattgcggg
     2821 ccgcttcggc gtctttgcgg gcgctaatat cgctgcgtag cgccataaaa cgctcaattt
     2881 ttccgtccgc gccacgtatg ggtgcaatca ccgtatcaaa ccaataaaaa tcgccatttc
     2941 tatgccggtt acacacctca ccccgccagg gctcgccact taatatttgc tgccatacct
     3001 ggccccaaaa gtctttgctg tgtaggcccg atttcataat gctgtggctt ttgccaatta
     3061 attcctccgg cctatagccg ctaaactggc agaaaggttc gttaacactt tggatcatgc
     3121 cgccttgatc agtaatagac agcaaaaatt gtgtattgat actttttaat agagcttcct
     3181 gttcagccaa ggtggcggcc aatttaattt tattattttt aagtatcgac acatcctgct
     3241 caacaaaata aaagccttta taagtatgtt gcgaaatggc cggaatcaat atccattgat
     3301 aatgggccaa gccctgctcc tgcaaatagg aatattgccc ggaattcacc tgcccctcgg
     3361 gcagataaaa cagccagggc gcgatttgcg cataatattc agccggtact acttccgcca
     3421 aacgctcccc cagtaaatta tgcccagaag aggcaaaacg ctcgcggctt ttttggttgg
     3481 ctttatagat gcggccatcc gcgctgacgg ccaataccgc ataaggcaag gcgttaacta
     3541 aggattccaa ctcatcgcgg gcctgtacca cctgcgcggt gcgcagggca acgcgggttt
     3601 ccaagtgggc atttaattct tttaattccc gctccgccgc acgcaattta ctcatatcgc
     3661 gaataatcgt cgcagcgcca attaccttat tctgctcgcc cagtagtggc gccgtataaa
     3721 ctaatacatc caataatgcg ccctgccctg tgcagcgctg ggccagggca tcggttttgg
     3781 tgcgctgctc gatggcagcc attaattcat tttccgcttg cgggcgctgc gcctcgggca
     3841 caatcagttg ggttaagggc tggccaaccg cttccaggct tgaaaagcca aacatagcct
     3901 cagcgcctcg gttccactgt tctatggtgc cgcttaaatt cacctcaata atcgcatcgg
     3961 ccgtcccctc gatcagcgct gccaagcggg cgttttgcgc ttgagtatgg cgcctttgag
     4021 ccagattgat atgtaaaatc gctgccaaaa aagccaatag caaagttgcg agcattccgc
     4081 ttataaatac caacttaggc tcggttaaat ccaattggcg caggtatttt tccgtcggcg
     4141 tgatggcaaa ttgccactgg cggccgaata tttcgctttg caataccacc gcagcaatcg
     4201 acttaagctc gatataatcc tcacacgcat aaatgcgttg gtgattgtca gtggtaatat
     4261 ccgagatcac ataatgaaaa agatcatcat taaaatgtgc gccgcgcaat atttcctgca
     4321 tcaccaaagg ttcgtatacc cagccaatat gcaggcgcgc gcggtcggct tcattgctag
     4381 gtatttgcca atcggcaaac accggcaaaa aaaacaaaaa ggcttgggta accttatcct
     4441 gcgcctgcat aagcgttatg ggcccagtta attgcggcgt attgcgcaca taggcccgct
     4501 cagcggcatc ccgccgcaat tcttcagaag cggtgtcgag cccccgcgct tgacggttat
     4561 taataatcgg ctcaatcaaa cgaataacat aacggtcgcc gtcatggggt tttataaaac
     4621 gcaggttaaa ggtatccaaa ccctcttgat gcatgcgctc aataaaggct tgctcatcgg
     4681 cgggcttgat gcggtcgatc acacctatgc cgcgcatacc ggggaattct tcggctaaat
     4741 taataaacga tacatactgc gcaaacgctt gcgggctggg gtggccattg tgggcgagca
     4801 caaaactgcg ggcgttgtag agcgcttggc ggtagctggc aaggcgcagc tcaaccttag
     4861 ccacttcttt ttgtgcatct tcctgcaggt ggcgctgcaa ttgtctctcg ttagccaaat
     4921 gaaattgcag cgcaaccaaa ccgctgaaca tcagcccaag cgcaacaagc gctatggcca
     4981 gcacaacagc tttttgtgtt cttgattgca cggtcattat ccacctaatt aatttttata
     5041 ttctcctgat aaaaccgagc gcgaaaattc acctcattct ggtagcgcta ttaggccatt
     5101 caatcgcagc cctgcacaaa ggacttgtag caagatgaat acatcctaaa aacaggccac
     5161 tataaaaagc ctggctatac ccagcgattc tttccatatg gcggtgaaca tttgccacgg
     5221 ggcgcggtct ggctagtctc gtgcgcgacc tatttacaca agggtaaaac cgctagcggt
     5281 aaataatcgt aattaattta ctgggcttat tcgtagtttt gttttggttt agtaggtttg
     5341 gtctaattgc cctgccttgc cccgatcagc ttaggccgca cacttaaccg tttacgtaac
     5401 gagcagcgca tgaaggatta tagccgcaag cctaacctgc gccagccgca gccagagtag
     5461 ctagcgtcta tcctaaactg cctgtaaagt gccaccccag tttcacccgc caatagttac
     5521 agccattgca gcgggcaaac atttcccatt gaaaaacatt cttattcgca gaaggctcaa
     5581 tgacacttcc ccccatttcc cgccctcgtc ttactattta ttgcgctgta tttattacgc
     5641 tatttgccaa ctgggctttg tggcaaaaac tgctgcaact gctaagcccc cttacccttg
     5701 atgaaatacc cttttgcctc tcggccctgg tactgatatt cgccatagcc cacggcgtgt
     5761 gccacctatt ggccctgggc cgtctgcaga aaatttggtt aattatcgcc ctggtatgta
     5821 gcgccgcagc cagttattac caggtgcagt accacgtgct gtacgataaa accatgattc
     5881 aaaatattct cgccacggat tatcgcgagg cctacggatt atttaatctt aaaatggtgc
     5941 tgtggattgc cctgttcggc ctgttgcctg caacaataat tagcctatgg ccaatcaaac
     6001 ctgcccccat taaaaagcag gccctacact atggcggcgg ttttttattg tcggtggtgc
     6061 tgattgccgc tgtagcagga attttttaca aagactatgc atcgctgttt cgcaataatc
     6121 gcgatttaaa acaccttatt atcccctcca gttttgttta ttccctgtat ggttttgtgg
     6181 ccgacaaaac caaaaccacc ggcgaacccg tgcaggtcat tggcagcgat gcccgcttag
     6241 gccccctctg gcaggccgcc aataaaccgg tggtgtttgt attggtggtg ggcgaaaccg
     6301 cccgcagggc caatttctc
//
LOCUS       DAOQGS010000206         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6111_length_6317_cov_4.021239, whole genome shotgun sequence.
ACCESSION   DAOQGS010000206 DAOQGS010000000
VERSION     DAOQGS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6111_length_6317_cov_4.021239"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..2017
                     /gene="mrdA"
                     /locus_tag="PKZ52_09120"
     CDS             98..2017
                     /gene="mrdA"
                     /locus_tag="PKZ52_09120"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNG59975.1"
                     /translation="MPFAAKPGEYRTFKNHFSEVRSFRFRLLFAAVLGLSLFAGLIAR
                     YYFLQVVHYEDFLTKAEQNRIQIQPVPPTRGLIYDTDNRVLADNRPSFMLTLVKERIA
                     DLDATLELLKSLIPISDADIDEYYKLLRQPRRPYEPIPLRFRLTEAEIATIAINEFRL
                     EGVEVEAQFVRYYPYADLFAHTVGYVGRISMNDLTSFDEDQVRRYAGSVSIGKVGVEK
                     AYEDELLGEVGARHVETNAHGRMLRVLDLNNPISGKDLHLYLDLDLQKVATAALGDNR
                     GALVALDVETGGVLAMVSTPSYDPNLFVTGISAADYKELNTSPLMPLYNRSLQGLYPP
                     GSTLKPMLGLGGLESGVITTGTRIYDPGFYTLPKDSHQYRDWKKGGHGGSVDLRQAIT
                     ESCDTFFYSLAANMGIDLMHPVGEAFGLGEKTGIDMPNERGGNWPSKEWKKSKKKLGW
                     FPGDSLNTSIGQGYVQATPMQLAVMTATLASRGIHRKPQLVKQIGDTPVPPQILSRYN
                     ARPSYWDYVINAMTDVVHSPQGTAKGIAAGLTYKIAAKTGTAQVVGIAQNERYDRNKV
                     RANNRDHALFIAFAPAYHPQIALAVIVENGEHGSSAAAPIARTLFDAYFAKQAAKKTP
                     PPDSGTPSAPAQVTP"
     gene            2014..3162
                     /gene="rodA"
                     /locus_tag="PKZ52_09125"
     CDS             2014..3162
                     /gene="rodA"
                     /locus_tag="PKZ52_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNG59976.1"
                     /translation="MKSLGPDFVRRLPENTEAFGKKNIRAGLIKIDFWLLFGLLLISS
                     FGITVLYSASGHSTAMAERQAIFFVLSYGVLLAVGQIRLGFLERWAVWLYCFGVVLLV
                     LTLVIGVGAKGAQRWISLGFTRFQPAEILKIAVPLTVAAYFSQRHLPPKFMHVVVCMI
                     IILVPSALILKQPDLGTAILVVMSGVIGLFLAGLRWRYIVLTLAVVAAAAWPMWNYVM
                     HDYQKKRVLTLIDPEADKWGAGWNILQSKTAIGSGGLEGKGYMKGTQSQLDFLPESHT
                     DFIIAVLAEEFGLQGVLALLACYAFVIGRGLVIAWRAPSTFGRILAGSISLTFLIYVF
                     VNMGMVSGLLPIVGVPLPMVSLGGTSLITLMLSFGLLMAVANDSRRAL"
     gene            3178..4158
                     /gene="mltB"
                     /locus_tag="PKZ52_09130"
     CDS             3178..4158
                     /gene="mltB"
                     /locus_tag="PKZ52_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNG59977.1"
                     /translation="MKIGLLLLGLLLSQVSFALGEYTQHPLTLAFIDKMVDQHQFNRK
                     QLLSLFAKVEKKQGILDAIARPAEKVKPWYEYRKIFITPERIAQGVQFWRENQTILSD
                     VERKYQVSAKMVVAIIGVETGYGRNTGKYRVLDALSTLAFDYPPRADFFVKELEAFLL
                     LTKQQQQDPLSLNGSYAGAMGYGQFMPSSVRNFAVDYNGDGVIDIWNNKGDAIASVAN
                     YFKANGWQYRAPVFTLAKKTASFNEQLLNTKAKPSRSLNELRSLGISPLTGDYPGSLP
                     AEALSYQLENSWEYWLGFNNFYVITRYNRSQMYALAAWQLGEAIEDARPQ"
     gene            4270..5361
                     /locus_tag="PKZ52_09135"
     CDS             4270..5361
                     /locus_tag="PKZ52_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNG59978.1"
                     /translation="MAAQLGRLKIAKPFIGLACLCLLLFTAACSSHKPVGTAHKPAES
                     TAPGEGAKKKGRYSLLEDQGPDVDIDVSNIPNAVPKKEDRTIAGNVSPYTVLGKTYYI
                     QFNAAKYSEIGLASWYGKKFHGERTSNGEIYNMFGMTAAHKTLPIPCYVRVTNLANNK
                     NVILRVNDRGPFHEDRVIDLTYTAAKKLDFHRAGTAKVRVDVINLNDFAPLPVAAVKS
                     DTSGKLTGALAGVANNNKAPEVNKTNTTAVAPIKTGVPVAQPAASKPASAAPAAVPLK
                     TASAKPAAFTPVYLQVGAFASQEGAAVLQKKLRSYTQLPIVLMPVEGKKIFRVLIGPI
                     ADANEMQALKQTLTEKKLSTPYVVQVEGK"
     gene            6037..>6317
                     /locus_tag="PKZ52_09140"
     CDS             6037..>6317
                     /locus_tag="PKZ52_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59979.1"
                     /translation="MNLITERFPIKLSLANVLFASLLAACGGDKVSDGQAQQNSNNGV
                     AQNDATLTEQEKIRRGFITLNRSSSSSSSSGKPVAALSSSSSTSSSGKLL"
BASE COUNT         1576 a         1487 c         1592 g         1662 t
ORIGIN      
        1 ggtgcgcatt gtgtgggtgg agcagttata ccgcgcccac agtattttaa taaatcaccc
       61 ctaccacaaa taacgcgcag gtttttgcag gaatttcatg ccctttgccg ccaagcccgg
      121 tgaataccgc acatttaaaa atcatttttc tgaagtgcgc agctttcggt ttcggctgtt
      181 atttgccgct gttttaggct tgagtttatt tgccggttta attgcccgct attattttct
      241 gcaggtagtg cactacgagg attttttaac caaagccgag caaaaccgca ttcaaattca
      301 gccggtgccg cctacgcgcg ggttaattta cgatacggat aatcgcgtgc tggcggataa
      361 ccggccgagt tttatgctca ccttagtaaa agagcgcatt gccgatttag acgccacctt
      421 agagttactc aaaagtctta ttcccatcag cgatgcggat atcgacgaat actataaatt
      481 gttgcgccag ccgcgcaggc cctacgagcc tattcccctg cgttttcggt taacggaagc
      541 ggaaattgcc accatcgcta ttaacgaatt tcgcttagaa ggcgttgaag tggaagctca
      601 atttgttcgt tattaccctt acgccgattt atttgcccac accgtaggtt atgtgggccg
      661 catcagcatg aacgatctca ccagtttcga tgaagaccag gtgcgccgct atgcaggctc
      721 cgtcagcatc ggtaaagtcg gtgtggaaaa agcctacgaa gacgaattat taggtgaggt
      781 gggtgcccgc cacgtggaaa ccaatgccca cgggcgtatg ttgcgggtat tggatttaaa
      841 caatcccatt tcaggcaagg atttacacct gtatttggat ttggatttac aaaaagttgc
      901 caccgcagcc ctgggtgata atcgcggcgc tctggtggcg ctggatgtgg aaaccggcgg
      961 tgtcttagcc atggtaagta cacccagtta cgacccgaat ttatttgtaa ccggcattag
     1021 cgcggcggat tataaagaat taaatacttc tcctttaatg ccactctaca accgcagctt
     1081 gcaagggtta tatccacctg gctccacctt aaaacccatg ttgggtttag gcggcctgga
     1141 atcaggcgtg atcaccaccg gcacgcgcat ttatgatccg ggtttttata ccctgccaaa
     1201 agatagccat caataccgcg actggaaaaa aggtggccac ggcggctcgg tggatttgcg
     1261 ccaggcgatt accgaatcct gcgatacatt tttttacagc ctggccgcca atatgggcat
     1321 tgatcttatg catccggtgg gagaggcctt tggcttaggg gaaaaaaccg gcattgatat
     1381 gcccaacgag cgcggcggta attggccgtc gaaagagtgg aaaaaatcca agaaaaaact
     1441 cggctggttc ccaggggata gtttaaatac cagtatcggc cagggttatg tgcaggcgac
     1501 ccccatgcaa ttggcggtga tgactgcaac cctcgcctcc aggggcatcc accgcaaacc
     1561 ccagttggtg aaacaaattg gcgatacacc tgtgccgccg caaattctta gccgctacaa
     1621 cgcgcgcccc tcctattggg attatgtcat caacgccatg accgacgtgg tgcattcacc
     1681 ccagggtaca gccaagggta ttgccgccgg tttaacctat aaaatcgccg ctaaaaccgg
     1741 taccgcccag gtggtgggaa ttgcccaaaa cgagcgctat gaccgcaata aggtgcgcgc
     1801 taacaaccgc gaccacgccc tatttatcgc ctttgctccg gcctatcacc cgcaaattgc
     1861 cctggcggtg attgttgaaa atggtgaaca cggctcctcc gctgcagccc ccattgcgcg
     1921 gaccttattc gatgcctatt tcgccaaaca ggccgctaaa aaaacgcccc cgcccgatag
     1981 tggcacccct tcggctcctg cacaggtgac cccatgaaat ccctcggccc cgattttgtt
     2041 cgccgcctgc cggaaaatac cgaagcgttt ggcaaaaaaa atattcgcgc aggtttaatt
     2101 aaaattgatt tttggctgtt atttgggctg ttattaatca gtagttttgg cattaccgtt
     2161 ttgtattctg cctctgggca ctccacggcc atggccgagc ggcaggcgat attttttgtt
     2221 ttgtcctacg gcgtattgtt ggcagtgggg caaattcgcc tcggcttttt agagcgctgg
     2281 gccgtttggt tgtattgttt tggcgtggta ctgctggtat taacgctggt aataggggtg
     2341 ggcgccaagg gcgcccagcg ctggattagt ttgggcttta cccgctttca gcccgctgaa
     2401 atattaaaaa ttgcggtgcc tttaacggtg gccgcgtatt tttcccagcg ccatttaccg
     2461 cctaaattta tgcatgtagt ggtgtgcatg atcatcattc tggtgcccag cgcattaatt
     2521 ttgaaacagc cggatttagg caccgcaatt ttagtggtga tgtcgggtgt tatcggcttg
     2581 tttttagccg gtttgcgctg gcgttatata gtgctcacct tggcggtggt ggccgcagca
     2641 gcctggccca tgtggaatta tgtgatgcac gattatcaga aaaaacgcgt gctcaccctc
     2701 atcgacccgg aagccgataa atggggcgcg ggttggaata ttctgcaatc aaaaaccgcc
     2761 atcggctccg gcggtttaga gggcaagggt tatatgaaag gcactcaatc gcagttggat
     2821 tttctgcctg aaagccatac ggattttatt atcgccgtgc tggcggaaga atttggcctc
     2881 caaggtgtgc tggccttatt ggcctgttat gcctttgtaa tcgggcgcgg tttggtgatc
     2941 gcctggcggg cgccttcaac attcgggcgc atattagccg gcagtatttc attgacgttt
     3001 ttaatttatg tcttcgtcaa tatgggcatg gtatctggcc tattgcccat tgtaggggtg
     3061 ccactgccga tggtgagttt aggtggcact tcattaatta cattgatgtt gagttttggt
     3121 ttattaatgg cggtggctaa cgatagccgc cgcgcactat aaaaaggtat acaggtcatg
     3181 aaaattgggt tgcttttgtt gggcttatta ttaagccaag tcagctttgc attgggggaa
     3241 tatacacagc atccgctcac cttggcgttt atcgataaaa tggtggatca gcaccaattt
     3301 aaccgcaaac aattgctcag cctatttgcc aaggtggaaa aaaaacaggg cattttagat
     3361 gcaattgccc gcccggcgga aaaggtaaaa ccctggtacg aataccgcaa aatatttatt
     3421 acccctgagc gtattgccca gggcgtacag ttctggcggg aaaatcaaac tattttgagc
     3481 gatgtggaac gcaaatatca ggtgagcgcc aaaatggtgg tagccattat aggtgtggaa
     3541 accggttatg gccgcaacac gggtaaatac cgcgtgctgg atgcactgtc taccctggcg
     3601 tttgattacc cgccgcgggc cgattttttt gtgaaagaac tcgaagcctt tttattgctg
     3661 accaaacaac agcaacaaga tcccttaagc cttaatggct cctatgcagg tgccatggga
     3721 tacgggcaat ttatgcccag cagcgtgcgc aattttgcgg tggattataa cggcgatggt
     3781 gtgattgata tttggaacaa caaaggcgat gccatcgcca gcgtggctaa ttattttaag
     3841 gccaatggtt ggcagtatag ggccccggtt ttcaccctgg cgaaaaaaac cgccagtttt
     3901 aatgagcaat tactcaatac caaagctaag cccagccgca gcctaaatga gttgcgcagc
     3961 ctcggcatca gcccgctcac tggcgattat cccggcagtt taccggcgga agcgctcagt
     4021 tatcagttgg aaaacagttg ggaatattgg ctgggcttta ataactttta tgtcatcacg
     4081 cgctataacc gcagccaaat gtatgctctg gcggcctggc aattgggtga ggcaatcgaa
     4141 gatgctcggc ctcaataggt gggagttttg agtttaagag gacgttcagt ggtacaggtt
     4201 ttaggcgata ttagggtatt aaacccagta atcagatgtg cggcacgcaa aggcgtttgc
     4261 cgtcaaaaaa tggctgcaca actgggccgt ttaaaaatag ccaaaccatt tattggcctt
     4321 gcctgcctgt gtttattatt gtttacagcg gcctgttcta gccataaacc tgttggtacc
     4381 gcccataaac ccgcagagag taccgcgcct ggtgaagggg cgaaaaaaaa gggccgttat
     4441 tccctgctgg aagatcaggg gccggatgtt gatatagatg taagcaatat tcccaacgcg
     4501 gtaccgaaaa aagaagatcg caccattgca ggtaatgtct cgccttatac ggtgctgggt
     4561 aaaacctatt acattcaatt taacgccgcc aaatactctg aaattggcct agcgtcctgg
     4621 tatggcaaaa aatttcacgg cgagcgcacg tccaacggtg aaatttacaa tatgttcggt
     4681 atgacggcag cccacaaaac cctccccatt ccctgttatg tgcgggtaac taatctggcc
     4741 aataataaaa atgtcattct gcgcgtgaat gatcgcggcc cttttcatga agatcgagtc
     4801 attgatctta cttatacggc cgctaaaaaa ttagattttc accgcgccgg gactgccaag
     4861 gtgcgtgtgg atgtgattaa tttaaatgat tttgctccac tgccggttgc tgcggtgaaa
     4921 agtgatacta gcggaaagtt aaccggcgct ttagccggag ttgccaataa caataaagct
     4981 cctgaggtga ataaaacgaa taccacggca gttgcgccga taaaaacggg tgtacctgtg
     5041 gcgcagcctg cggcgagcaa acccgcttca gcggcgcctg ccgcagtgcc cttaaaaacc
     5101 gccagtgcta aacctgcggc atttacaccc gtgtatttac aagtgggggc gtttgcctcg
     5161 caggagggcg ccgctgtact gcaaaaaaaa ctgcgcagct atacacaatt gccgattgtg
     5221 ttaatgcctg ttgaggggaa aaaaatattt cgtgtattaa tagggcccat tgctgacgca
     5281 aacgaaatgc aagcgttgaa acaaactcta accgagaaaa aactctctac accttatgta
     5341 gtgcaagtgg aaggcaaata gtaagtgagt cttagcttat tgagggcctg cactcgattg
     5401 attacctatc aacgtttact accaatccaa cttagtccaa caggtactta agcgtgccaa
     5461 gccttttgcc atatgctcca caaaatgtgt aaaaaagtac gaatacagcc aatttgatga
     5521 tttaatgaac agcggttgtg tgaactttgt tgcaatttag gtggtaagcc ttgttataat
     5581 tccgcggtca aaatccgcgc tgtcgaaata ctttccagta taagagcggc agtgcgagtc
     5641 ctatgtaagt catacaagtc gctatttagg atagaggctt ttttcaggga atcttgttcc
     5701 agcgagtacc taaataacca aatctgtagt catgctgtgt atacccgtaa tttggctata
     5761 gggtgcgctt gcttacggtt ctaattgtgt tgaaccctcg tgtatttagg tataagcctt
     5821 attccggttt cagtggattg gtttttttta agaattattt agaaaaccag cttgagattt
     5881 aacgcacaaa actcatgttt tatcggtaaa tttacgtttt ctgagggagg agagcccggt
     5941 cgtggataat ttctatgcta tgaattattt acgacccgcg gtgcttggtc atccaaagtc
     6001 gactcttgat ttacatgatg atgtagggtg tacacagtga atttgataac cgaacgcttt
     6061 cccataaaat tatccttggc taatgtgctg ttcgcgtcgt tgctggccgc ctgtggtggc
     6121 gataaggttt cagacggaca ggcacaacag aattccaata atggtgttgc tcaaaacgac
     6181 gcaaccctga ccgagcaaga gaaaatccgt cgcgggttta tcacgctcaa tcgttcctcc
     6241 agttcctcaa gctcgtcagg aaagcccgtc gcggcgctgt ccagctcaag ttcaaccagc
     6301 tcctcaggaa aattgct
//
LOCUS       DAOQGS010000207         6294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6147_length_6294_cov_3.971951, whole genome shotgun sequence.
ACCESSION   DAOQGS010000207 DAOQGS010000000
VERSION     DAOQGS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_6294_cov_3.971951"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..771
                     /locus_tag="PKZ52_09145"
     CDS             448..771
                     /locus_tag="PKZ52_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNG59980.1"
                     /translation="MINLTESAVGAVRRFIDSSEKAIVGLRIKVEGGGCSGLKYGMKL
                     EETVEQDDSIVDFPGVRVVIDPASVPLLENVTVDFLDGLEGSGFKFENPNATASCGCG
                     KSFAC"
     gene            844..1770
                     /gene="nifU"
                     /locus_tag="PKZ52_09150"
     CDS             844..1770
                     /gene="nifU"
                     /locus_tag="PKZ52_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNG59981.1"
                     /translation="MWDYSEKVKEHFYNPKNAGAVEAANAVGDVGSISCGDALRLSLK
                     VDPATEIILDAGFQTFGCGSAIASSSALTEMVKGKTLDEALLISNQDIADFLDGLPPE
                     KMHCSVMGREALQAAVANYRGEEWSDDHAEGALICKCFAVDEVMIKDTIRANNLSTVE
                     EVTNYTKAGGGCSSCHEGIEKILSEMLAERGEVFTPGAVGKKAAAATKSKPVIAVKPA
                     TGPLTAVQRILKIEEVLEAIRPNLRADKGDVELVDVEGKTVYVNLIGACSGCQLAAST
                     LGGIQQKLIEALGEFIKVVPNSERQSLVQVGA"
     gene            1774..2982
                     /gene="nifS"
                     /locus_tag="PKZ52_09155"
     CDS             1774..2982
                     /gene="nifS"
                     /locus_tag="PKZ52_09155"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNG59982.1"
                     /translation="MSDIYLDNNATTAVDPVIVDAMLPFFTEQFGNPSSMHNFGNKVG
                     VAIKKARKSIQNLLGAEHDSEIIFTSCGTESDSTAILSALKSQPERKEIITTVVEHPA
                     ILNLCDYLATEGYTVHKLPVDNKGRLNLDEYTKLLTDKVAIVSVMWANNETGTYFPVI
                     KMAEMADAAGVLFHTDAVQAVGKIPMNLQDTSIHMLSLSGHKLHAPKGVGVLYLKRGV
                     RFRPLLRGGHQERGRRAGTENSAGIVGLGVAADLALQHMEYENTQVAAMRDHLQAGLL
                     AAIPHAFVTGDPDNRLPNTLNIAFEYIEGEAILMLLNKAGIAASSGSACTSGSLEPSH
                     VMRAMGIPYTAAHGTVRFSLSRYNTLAEMDRVIEVVPPIIAQLRKLSPYWGVDGPLEN
                     PEQAFAPVYA"
     gene            3013..4182
                     /gene="nifV"
                     /locus_tag="PKZ52_09160"
     CDS             3013..4182
                     /gene="nifV"
                     /locus_tag="PKZ52_09160"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNG59983.1"
                     /translation="MPKQPASIVIDDTTLRDGEQSAGVSFSAEEKLAIAQGLAAIGVA
                     ELEIGIPAMGAEEREVMAAIVDLKLPAHLLAWCRMGDEDLLAVQGLGVHMVDLSIPVS
                     DQQIQHKLGRNRAWVLTQINQMVRKARMLGYQVCVGMEDASRADMEFILQVADAASDA
                     GAARLRFGDTVGIMEPFSVHSKISRLRASCDLELEMHAHDDFGLATANTLAAVAGGAT
                     HINTTVNGLGERAGNAPLEECVLALKHLHGIDTGVDSKGVPALSQLVERASGRAVGWQ
                     KSIVGPGVFTHEAGIHVDGLLKDPRNYQGLDPSELGRSHQLVLGKHSGKHMLRRAYQE
                     AGIELEDWQITPLLQQVRNQVTRTKRSPGTSELLYLYQSLTRLMATPLTQLGAPV"
     gene            4179..5006
                     /gene="cysE"
                     /locus_tag="PKZ52_09165"
     CDS             4179..5006
                     /gene="cysE"
                     /locus_tag="PKZ52_09165"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNG59984.1"
                     /translation="MNASVNLPGALTGAPSSKGLFAQWREDVACVFQRDPAARSVFEV
                     LTTYPGVHAVIVHRLSNRLWLAGWRYAARWLSFVARLLSNVDIHPGAAIGARFFIDHG
                     ACVVIGETAIIGDDVTLYHGATLGGTRWTKGKRHPTLGNGVMVGAGAKVLGDITLGDN
                     SRVGANSVVIEDVPEGCTVVGIPGKIVKIREAGLPNAHGIDLDHHLIPDPVGKAIACL
                     LSRIDALEMKLNRQAALDHYDSCTVDNSICEQDCPGGARAKDTVSTLPRQPIVRGAR"
     gene            5006..5530
                     /locus_tag="PKZ52_09170"
     CDS             5006..5530
                     /locus_tag="PKZ52_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59985.1"
                     /translation="MDLQNFEGQLLYFDLNHSEEVTALLQRAAHAYGEGAAEPLLKQA
                     YALAPQDLTVLVALYRFYYYQHRYQDALDIAFQAMRAVAPTISFPADWLKLSFTHLAN
                     GVLVSFTLVRFYLLALKGAAYLNLRMGNIADGIYMLKKVVEFDSKDRLGAKALLQSIS
                     PAPVADNTRLRATR"
     gene            5538..5888
                     /locus_tag="PKZ52_09175"
     CDS             5538..5888
                     /locus_tag="PKZ52_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HNG59986.1"
                     /translation="MEPNQTSSLLDDLEELSSAEDFLNYFQIPFDTTVVLVNRLHIMQ
                     RYHDYLSQAQASLAEYEGNDAAIAAVYKSLLTQAYEDFVHSDAKTEAVLKIYKMQGPQ
                     TTFVSLDSLMWDLK"
BASE COUNT         1484 a         1774 c         1697 g         1339 t
ORIGIN      
        1 tccctgcagt cgtccgcttt ttttgccaaa ttcgcctagc cagcgcttcg ccgcctacat
       61 ttttcagagg ttccttaatt cagttgctat agcttaatga tatctcctca gctcaacgtc
      121 attgcctcaa cttaattcca ttgcattaaa aagaataaat agcataaggg cggcacggct
      181 gcgtatttac taatagtcgc ttgccgttaa tgcgccatcc ccccctacaa accaccccaa
      241 ttgcctctgt agcaaatgcg acaagcccgc aagcaagttg taattactac acacaggcag
      301 cctgtggtta taggctgcaa gctactgaaa aataaccatt taacggcagt tttaggcgct
      361 ggcatgtggc ttgcaatttc aggcttgaat aactgcattc cctgggcagt tgaaaaattt
      421 agccccattt tcaatggagt aacgcacatg atcaacctta ctgaaagtgc cgttggcgct
      481 gtacgtcgtt ttattgattc ctctgaaaaa gccattgtag gcctgcgtat taaagtcgaa
      541 ggcggcggct gctccggact taaatacggt atgaaattgg aagaaaccgt tgaacaagac
      601 gattccattg tggattttcc cggtgtgcgc gtggtgattg accctgccag tgtgcccctg
      661 ttggaaaatg tcactgtgga ttttctcgac ggtttagaag gcagcggctt taaatttgaa
      721 aaccctaatg ccacagccag ttgcggttgc ggaaaatcgt ttgcctgcta attgaacgaa
      781 gcagaattaa ctgctgttaa ttttcctcaa cactcatcca tcacattttg gagcatcgat
      841 gccatgtggg attattctga aaaagtgaaa gaacattttt acaaccccaa aaacgccggt
      901 gccgtggaag cggccaatgc agtgggcgat gtgggttcca tcagctgcgg cgacgccctg
      961 cgtttatcgc tcaaagtaga ccctgcaact gaaattattc tcgatgctgg gtttcaaacc
     1021 ttcggttgcg gatcggccat tgcgtcatca tctgctttaa ccgaaatggt aaaaggcaaa
     1081 accttggacg aagcgctatt aatttccaac caagatatcg ccgacttttt agatggcctg
     1141 ccgcctgaaa aaatgcactg ctccgtgatg ggccgcgaag ccttacaagc ggcggtagcc
     1201 aattatcgcg gtgaggagtg gagcgatgat cacgctgaag gcgcattaat ttgcaaatgt
     1261 tttgcggtgg atgaggtgat gattaaagac accatccgcg caaacaatct ctccacggtg
     1321 gaagaagtga ccaactacac caaagccggt ggtggctgtt cttcctgcca tgaaggcatt
     1381 gaaaaaatcc tctcagaaat gctggccgaa cgcggtgaag tatttacccc cggcgcggtg
     1441 ggcaaaaaag cggctgctgc caccaagagc aaacccgtta ttgctgttaa acctgcaacc
     1501 gggccgctta cggctgtgca acgcatttta aaaatcgaag aagtgctgga ggccattcgc
     1561 cccaatttac gcgccgataa aggcgatgtg gaattggtag atgtggaggg taaaaccgtg
     1621 tatgtgaatt taattggcgc ttgtagcggc tgccaattag cggcttccac cctcggcggt
     1681 attcagcaaa aacttattga agccttaggt gagtttatta aagtcgtgcc caacagcgaa
     1741 cgccaatcct tagtgcaagt gggagcataa gttatgagcg acatctattt ggataacaac
     1801 gccaccactg cggtggaccc ggttattgtt gacgccatgt tgcctttttt taccgagcaa
     1861 tttggcaacc cgtcttccat gcataatttt ggcaacaaag taggtgtcgc cattaaaaag
     1921 gcgcgcaaaa gcattcaaaa cctcttgggt gccgagcacg attcagaaat tatttttacc
     1981 tcctgcggca ctgagtccga ctccactgca attttatcgg cacttaaatc tcagccggag
     2041 cgcaaggaaa ttatcaccac cgtggtagag cacccggcca ttttaaattt gtgcgattac
     2101 ctggccaccg aaggctacac cgtgcataaa ttgccggtgg ataataaagg ccgcttaaat
     2161 ttggatgaat acaccaagtt gctcactgat aaagtcgcaa ttgtgagtgt gatgtgggcc
     2221 aataatgaaa ccggcaccta tttccccgtg attaaaatgg cggaaatggc cgatgcagcc
     2281 ggggtgttat tccacaccga tgcggtgcaa gcagtgggca aaattcccat gaatttgcaa
     2341 gacaccagca ttcatatgct gagtttgtcc ggccataaat tacacgcgcc aaaaggcgtt
     2401 ggtgtgttgt atttaaaacg cggcgtgcgc tttcgccctt tattgcgcgg tggccaccaa
     2461 gagcgcggcc gcagagcggg cactgaaaac agtgccggca ttgtgggcct gggcgtggcg
     2521 gcggatttag ccctgcaaca tatggagtat gaaaataccc aagtggccgc catgcgcgat
     2581 catttacaag cgggcctatt ggcggcaatc ccccatgcct ttgtcactgg ggacccggac
     2641 aaccgactac ccaataccct caatatcgcc tttgaatata ttgaaggtga ggccattttg
     2701 atgctgctga ataaagcagg catcgccgcc tccagcggtt cggcctgtac ctcaggctcc
     2761 ctagagccct ctcacgtgat gcgcgccatg ggtattccct acaccgccgc ccacggcacc
     2821 gtgcgcttct ccctctcccg ctataacacc ctggcggaga tggaccgggt gattgaagtg
     2881 gtgccgccta tcatcgccca gctgcgcaag ctctccccct actggggtgt ggacgggccg
     2941 ctggaaaacc ccgagcaagc ctttgcccct gtgtacgcct aatccatccc tcacctgctt
     3001 aagtaccgcc ccatgcccaa gcaacctgcc agcatcgtca tagacgacac caccctcaga
     3061 gacggcgagc aaagcgccgg ggtgtcgttt agtgccgagg aaaaactcgc catcgcccag
     3121 ggccttgctg ccattggtgt ggcggagctg gagatcggca ttcccgccat gggggccgaa
     3181 gagcgggagg tgatggcagc gattgtggat ttgaaactgc ccgcgcacct cttagcctgg
     3241 tgccgcatgg gggatgagga tctactcgcc gtgcagggtt tgggagtgca tatggtggac
     3301 ctatccatcc cggtgtccga ccagcaaatc caacacaaac taggccgcaa ccgcgcttgg
     3361 gtgctcacgc aaattaacca gatggtgcgc aaagcccgca tgctggggta tcaagtgtgt
     3421 gtggggatgg aagatgcctc ccgggcggat atggagttca tcctgcaagt ggcggatgcc
     3481 gccagcgatg ccggcgccgc acgcctgcgc tttggcgaca cagtgggcat catggagccc
     3541 ttcagtgtgc acagcaaaat aagccgcctg cgagcaagct gcgatttaga gctggagatg
     3601 cacgcccacg acgattttgg cctggccacg gccaataccc tcgccgccgt tgccggcggc
     3661 gctacccaca tcaataccac cgttaacggc ctgggtgaac gggcgggcaa tgcgccctta
     3721 gaggaatgtg tgctggccct taaacacctg cacggcattg atacaggggt agacagcaag
     3781 ggcgtgcctg cgctgtcgca actggtggag cgcgcctcgg gccgagccgt gggctggcaa
     3841 aaaagtattg ttggccccgg cgtgttcacc cacgaggcgg gcatccatgt ggatggcctg
     3901 ctgaaagacc cgcgcaacta ccagggcctc gaccccagtg aactcggccg cagccaccag
     3961 ttggtgctgg gtaaacactc cggcaaacac atgctgcgcc gggcctatca agaggcgggc
     4021 attgagctgg aggactggca aatcaccccc ctgctgcaac aggtgcgcaa ccaagtcacc
     4081 cgcaccaagc gcagccccgg cacaagcgag ctgttatacc tctaccaaag cctgacccgc
     4141 ttaatggcaa ccccccttac acaactagga gcgccggtat gaacgcatcg gtaaatctgc
     4201 ctggcgccct taccggcgcc ccgtcatcca aaggcctatt tgcccagtgg cgagaagacg
     4261 tagcctgtgt attccaacgg gacccggcgg cccgcagtgt atttgaagtg ctcaccacct
     4321 acccaggtgt gcatgcggtg attgtccatc gcttaagcaa ccgcctgtgg ttggccggtt
     4381 ggcgctacgc cgcccgctgg ctgtcctttg tggcgcggct gctcagcaat gtggacatcc
     4441 accccggcgc cgccattggt gcgcgctttt ttatcgatca cggcgcctgt gtggtgatcg
     4501 gcgaaaccgc catcattggc gacgacgtta ccctctacca cggtgcaacc ctgggcggca
     4561 cccgctggac caaaggcaaa cgccacccca ccctgggcaa cggcgtgatg gtgggggcag
     4621 gcgccaaggt gctgggggat atcaccttgg gggataacag ccgggtgggc gccaactccg
     4681 tggtcattga agatgtgccg gagggctgca ccgtagtggg catccccggc aagattgtaa
     4741 aaatccgcga ggcgggcctg cccaacgccc acggcataga cctagaccac cacctgattc
     4801 ccgaccctgt aggcaaagcc atcgcctgcc tgctcagccg catcgacgcg ctggaaatga
     4861 aactcaaccg ccaggccgcc cttgatcatt acgacagctg cacagttgat aacagcatct
     4921 gcgagcagga ctgcccgggc ggcgctcgcg ctaaagacac agtttcgacc ctgccacgcc
     4981 aacctatagt acgaggtgct cgctaatgga cctgcaaaac tttgaagggc aattgctcta
     5041 tttcgacctg aaccattccg aggaagtaac cgccctgctg cagcgcgccg cccacgccta
     5101 tggcgaaggt gccgccgagc cactgctcaa acaggcctac gccctagcac cccaggactt
     5161 aaccgtgttg gtggccctgt accgctttta ttactaccaa caccgctacc aagatgcctt
     5221 ggacatagcc tttcaagcca tgcgggcggt ggcacctacc atcagcttcc cggcggattg
     5281 gttgaagctg tcgtttactc acctggccaa tggcgtgttg gtgtccttca ccctggtgcg
     5341 cttttacctc ttggccctca agggcgcggc ttatttgaac ctgcgcatgg gcaacatagc
     5401 cgatggcatc tacatgctga aaaaggtggt ggagttcgac agtaaagatc gcctgggggc
     5461 caaggccctg ttgcaatcca tcagccctgc ccccgtggca gacaacaccc gcctgcgcgc
     5521 cacccgctga ggtcgttatg gaaccgaatc aaaccagtag cttgctcgac gatttagaag
     5581 aactcagttc agcggaggat ttcctgaact acttccaaat cccctttgat accaccgtgg
     5641 tgctggttaa ccgcctgcac atcatgcagc gctaccacga ctacttaagc caagcccagg
     5701 catccctggc cgagtacgaa ggcaacgacg cggccatagc cgccgtctac aaaagcctgc
     5761 tcacccaggc ctatgaagac ttcgtgcact ccgacgccaa gactgaagcc gtgctgaaaa
     5821 tctacaaaat gcaggggccc cagacgacgt ttgtctcttt ggattccttg atgtgggatt
     5881 tgaagtaggg gttggtttaa gcgcctggaa atgttcagcc gccggtggag cccatagacg
     5941 agtaagagcc gtaggggcac cccttgtggg tgcccactcc caggcaaatt gagatggaca
     6001 tgctggctaa gaacagggca accacaaggg ttgcccctac ggcctcgcct tagctcaacg
     6061 atcgtggggc aggacgaaga tatgcattcc cacgcggagc ctgggaacga gaagaaaagc
     6121 gagaagagag aaaagtaacg agaaaagcgt tggaacaagt aaagccgaga atcgaacagg
     6181 tttttagccc ctcccccttt taaaggggga ggctgggagg gggttttata ggtgctcgca
     6241 cgcaggcaga aacgaacagt gaaccccacc ccaaccctcc ccttaaaaag ggga
//
LOCUS       DAOQGS010000208         6252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6208_length_6252_cov_3.898661, whole genome shotgun sequence.
ACCESSION   DAOQGS010000208 DAOQGS010000000
VERSION     DAOQGS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6208_length_6252_cov_3.898661"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PKZ52_09180"
     CDS             <1..1375
                     /locus_tag="PKZ52_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG59987.1"
                     /translation="PRQSGAEVNIADGQSAKTFVIEDGLGGRISRTLTPSRGANAGPI
                     ESITFAGFDFGQSVVKANPRLVANREALLRIHVLADQAQTAPAGTLTLKLGEQSQVVN
                     LTKPTSLPSLLQLTSLSRSYTAVVPANWVQPGLSLQMQLANGLSRTLAPVVGTGSVLY
                     LTLVPITSGGSSPQMPTAAAVERNLKTIWPLAQVVVRSHEPVDFSFPSADEGLAQLAD
                     LQAREGDTSYVFGLVSPAAPNFNVGGLAYVGGRVGMGLDATHDPSGLTMAHEIGHMFG
                     LFHVNCGSPSGIQNDYPYATTNIGSVGINYSLNRLILPTSASDVMSYCQPQHVSDFSY
                     QRAQSHLETNPPIPFPATSLSRAAAAPQLQSFVVRGHLTGAGEWVIRQALPSALPVEE
                     VPAGDYSAEVTDTQGKIYSAELRFMRIDHDEQISNRHFSISLPVLEGQKLVIRRRGEV
                     VYEGQLP"
     gene            complement(1416..2420)
                     /gene="arsB"
                     /locus_tag="PKZ52_09185"
     CDS             complement(1416..2420)
                     /gene="arsB"
                     /locus_tag="PKZ52_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNG59988.1"
                     /translation="MGIFERYLSLWVALAMGGGVLLGLWQPGWFAAVASFSLARINLP
                     VALLIWLMIYPMMIQIDFTAMQLRREHRQGLMLTLTINWLIKPFSLWALGSVFFFWLY
                     KSLISPEQAADYLAGLILLGVAPCTAMVFVWSQLTRGDANYTLMQVGTNDIIMLFAYA
                     PIAGFLMGLSNIHIPWDTLILSLVLYVLLPLLAGIATRRLLGSSEKINNFCHSLKPLS
                     VTALIGTVLLLFALQANAIAQQPLIIALIAIPLTLQTYGIFGLCWLGARLLRLPLSIS
                     APACLIGTSNFFELAVAVAIGLFGLDSGAALATVVGVLVEVPIMLSLVYYLNRQGQKA
                     "
     gene            complement(2420..2896)
                     /locus_tag="PKZ52_09190"
     CDS             complement(2420..2896)
                     /locus_tag="PKZ52_09190"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNG59989.1"
                     /translation="MKLLFICTHNRCRSILSEAITNHLGAGVIQAFSAGSAPAGQVHP
                     LTLKFLQEAHIPTQGLASKSWDLFADLNPTAVITLCDSAAAESCPLWMGDSLRLHWGL
                     ADPSKLAEDETAARQAFNACIAQIKTRTQALVALHGRGLQGDELKTALTQLVSEVA"
     gene            complement(2933..3253)
                     /locus_tag="PKZ52_09195"
     CDS             complement(2933..3253)
                     /locus_tag="PKZ52_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNG59990.1"
                     /translation="MNPLELFKALADPTRLACVLTLQARGELCVCELHELLAVSQPKM
                     SRHLAQLRKAQVLTQRKKGLWVYYQLNPQMPAWALQIIQHTALGNRHLIPAANAVCGP
                     GNIC"
     gene            3633..4886
                     /locus_tag="PKZ52_09200"
     CDS             3633..4886
                     /locus_tag="PKZ52_09200"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNG59991.1"
                     /translation="MSVTEYMTNLGSQARKASRVMAAADTQTKNQALIAIAGALEAQR
                     AALKAANALDMQRGRDNGLEPALLDRLELSDKVINSMIEGLRQVAALPDPIGAITDMN
                     LRPSGIQVGKMRVPLGVVGIIYESRPNVTIDAASLCLKSGNAAILRGGSEAIESNQAI
                     AACIAQGLSAVGLPESVVQVVNTTDRAAVGALISMPEYVDVIVPRGGRSLIERISLEA
                     RVPVIKHLDGICHVYIDAEADFEKSFNIAMNAKTHRYGVCNAMETLLVHQFAAPQILP
                     RLAAALIEKGVELRGCATTCTLINALAATEEDWRTEYLAPVLSIKVVEHLDEAISHIN
                     TYSSMHTDSIVTENYTLARRFLREVDSASVMVNASTRFADGFEYGLGAEIGISTDKIH
                     ARGPVGLEGLTSQKWIVFGDGQVRQ"
     gene            4899..5567
                     /gene="nadD"
                     /locus_tag="PKZ52_09205"
     CDS             4899..5567
                     /gene="nadD"
                     /locus_tag="PKZ52_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNG59992.1"
                     /translation="MERTQSPLLVYGGSFDPVHNGHVAAFWLAAHLLNAGEARLLPCF
                     LSPLKSQVFTTPEQRCALLQLVVTGLNQRSSACLFSLDRREIEIDGPSFTCDTLAALR
                     QEQGETRPIIWLLGDDAWRQLPQWRHWQRLTEFAHLLIAARPGVEAPIEPAQTAWAAA
                     RQCALAQLGLSPSGGIAHMNNALLAISASHIRTQIQQGVRPLGLLPDAVEERIYQQGL
                     YLNS"
     gene            5582..5929
                     /gene="rsfS"
                     /locus_tag="PKZ52_09210"
     CDS             5582..5929
                     /gene="rsfS"
                     /locus_tag="PKZ52_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNG59993.1"
                     /translation="MTDLNEVVITALENLKGQKIVNLNVRDLSDVTDCLIIATGTSSR
                     HVKSLAHNVVEEAKEKGFRPLGIEGLQEGEWALVDFGDTVVHVMSAEAREFYDLEKLW
                     SKVPANRTPDSKA"
     gene            5932..>6252
                     /locus_tag="PKZ52_09215"
     CDS             5932..>6252
                     /locus_tag="PKZ52_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNG59994.1"
                     /translation="MRVQLVAVGTKMPAWVQSGFTEYAKRLPKELTPKLLELELGSKS
                     KPGNMEAAKKAEAEAILAAIPAEHFVVMLDVGGKAWSTEELAAQLAQWQMSGRDFSFV
                     IGGPD"
BASE COUNT         1389 a         1791 c         1827 g         1245 t
ORIGIN      
        1 cccccgccag tctggcgctg aggtgaatat tgccgatggc caaagcgcca aaacctttgt
       61 gattgaagat ggcttgggcg gtcgcatcag ccgcaccctc acgcccagcc gcggcgccaa
      121 tgcgggcccc atagaaagta ttacgtttgc cggtttcgat tttggccaga gcgtagtgaa
      181 agccaacccc cgcctggtgg ctaaccgcga agccctgctg cgcattcatg tgctggctga
      241 ccaggcccaa accgccccgg caggcaccct gactttgaaa ctcggcgagc agagccaggt
      301 ggtgaacctc accaaaccca ccagcctgcc aagcctgctg cagttgacca gcctctcccg
      361 cagctacacc gctgttgtac ctgccaactg ggtgcagccg gggttgagcc tgcagatgca
      421 attggccaat gggctcagcc gcaccctggc gccggtggtg ggcacgggct cggtgttgta
      481 cctcaccttg gtgcccatca cttcaggcgg ttccagcccc cagatgccca cggcggcagc
      541 ggtggagcgc aaccttaaaa ccatctggcc cctagctcag gtggtggtgc gcagccacga
      601 accggtggat ttcagtttcc cctcggcgga tgaaggcctg gcgcagcttg cggatctgca
      661 ggcgcgggag ggggatacca gttatgtgtt tggcctggtg agcccggcgg cacccaactt
      721 taacgtcggc gggctggcct atgtgggcgg tagagtgggc atggggttgg atgccaccca
      781 cgacccttcc ggcctcacca tggcccatga aatcggccat atgtttggct tgtttcacgt
      841 caactgcggc agcccctcgg gcattcaaaa cgactacccc tatgccacta ctaacatcgg
      901 cagtgtgggc atcaactaca gcttgaaccg gttaattctg cccaccagcg cctccgatgt
      961 catgagttat tgccagcccc aacacgtgtc ggatttctcc taccaacggg cgcagtcaca
     1021 cctggagacc aacccgccga ttccctttcc cgccaccagc ctcagccgcg ctgcggctgc
     1081 gccgcagtta cagagttttg tggtgcgcgg tcatttaact ggggcggggg agtgggttat
     1141 ccgccaggcc ttgccctcgg cactgccggt tgaagaggtg ccagcggggg actacagcgc
     1201 cgaggtgacg gatacccagg gcaagatcta ctcagccgaa ctgcggttta tgcgcatcga
     1261 ccacgatgag cagatcagca accggcattt ctccatcagc ctgccggtgt tggaaggaca
     1321 aaaactggtt attcgccgtc ggggggaggt ggtttatgag gggcaattgc cttgaccacc
     1381 cacagagaat gctagacatc ggccgccaaa gaggcctagg ccttctgccc ctgccggtta
     1441 aggtaataca ccagggataa cataatcggc acttccacca ataccccaac caccgtagcc
     1501 agagccgcac cggaatctaa gccgaagagg ccaatggcca ccgccaccgc caattcaaaa
     1561 aagttcgagg tgccgattaa acaggccggt gcgctaatag acaggggcag tcgtaagagg
     1621 cgcgccccca gccagcagag cccaaaaata ccgtaggttt gtagggttag gggaattgcg
     1681 ataagggcga tgataagtgg ctgttgggcg atggcattgg cttgcagggc aaacagcaat
     1741 aacacagtgc cgattaaggc tgtcactgac agtggcttaa ggctgtggca aaaattgttg
     1801 attttttcgc tactgccaag caatcggcgg gtggcgatgc ctgccaagag cggcaataac
     1861 acatacaaca ccagggacaa aataagtgtg tcccagggga tgtgaatatt gctcaggccc
     1921 atcaaaaacc cggcaatcgg cgcataggca aacaacataa tgatgtcgtt ggtgcccact
     1981 tgcatcaggg tgtaattggc atccccacgg gttaactggc tccacacaaa caccatggca
     2041 gtgcaggggg caacgcccaa gagaatcaag ccagccaaat aatccgccgc ctgctcaggg
     2101 ctaatcaggc ttttatacag ccagaagaaa aacacgctgc ccagggccca gaggctaaag
     2161 ggtttgatca gccagttgat ggtgagggtg agcatgagcc cctggcggtg ttcccgccgc
     2221 aactgcatgg cggtaaagtc gatctgaatc atcatggggt agatcatcaa ccagatcagc
     2281 agagccaccg gcaggttaat ccgcgccaga gaaaaactcg ccacagcggc aaaccagccg
     2341 ggctgccaaa gccccagcag cacaccgcct cccatggcaa gggccaccca gaggcttaaa
     2401 tagcgctcga atatgcccat taggccacct cactcaccag ctgcgtcagc gccgttttta
     2461 actcatcacc ctgcaggcct cggccgtgta aggctactaa agcctgagtg cgggttttga
     2521 tctgggcgat gcaggcatta aaggcctgcc gtgcggcggt ctcatcctcg gcaagtttcg
     2581 acgggtccgc caggccccag tgcaggcgca ggctatcgcc catccacagg gggcagctct
     2641 ccgccgccgc gctatcacat agggtgatca ccgcagtggg gtttaagtcg gcaaacaggt
     2701 cccaggactt actggctagc ccctgggtgg gaatatgggc ctcctgcaag aattttaagg
     2761 tgagagggtg cacctgcccg gcgggggcgc tgcctgcact aaaggcctgt attactccgg
     2821 cgcctaagtg gttggtgatc gcctcagaga gaatactgcg gcagcggttg tgggtgcaga
     2881 taaagagtaa tttcatgggg tacctatata ggacgtttca aatgtatatg gcttaacaga
     2941 tattacccgg gccgcaaact gcgtttgcgg cagggattaa atggcggttg cccagggccg
     3001 tgtgctgaat aatctgcagc gcccaggcgg gcatctgggg atttaattgg tagtagaccc
     3061 aaaggccctt tttgcgctgg gtgagtactt gagccttgcg caattgagcc aaatggcggc
     3121 tcattttcgg ctggctgacg gcgagtaact cgtgcaattc acacacgcac aattcccccc
     3181 gcgcctgcag ggttaacaca caggctaagc gggtgggatc ggcgagggct ttaaacaatt
     3241 ccagagggtt cacagttggc ttccggctca ttcacggcgg gcactatata cggtaaggcg
     3301 tatttattac aggagcgtgt ccgttacaga gcgctgtttg tgcgaacaag ctgggataag
     3361 ggcctgggca cctgggacat caccaggggg aggaagccca ggcagagcaa gcgaaatgcg
     3421 ggttttcctg cccagccgaa cgattcacct gtgttgtacc gcgaatcgag agtgtcccac
     3481 ttcttagtgt gcagctcatt tagcagagtg caattatgag cgcctagcca ggtgttacac
     3541 cccgataaac aacaccgcaa tcgccgcgat taatgcaggt gcaggccggt gagcgctggt
     3601 atactggccg ctttttgctg cggaacacat ttatgtcagt caccgagtac atgaccaacc
     3661 tgggcagcca ggcccgcaaa gcgtcccgag tcatggccgc cgccgatact caaaccaaaa
     3721 accaagccct tatcgccatc gcaggtgcac tcgaagccca gcgcgccgcc cttaaagccg
     3781 ctaatgcact ggacatgcaa cgcggccggg acaatggctt ggagcccgcc ctgctcgatc
     3841 gcttagagct gtccgataaa gtcattaaca gcatgattga gggcctgcgc caagtggctg
     3901 ccctgccgga ccccatcggt gccatcaccg atatgaacct gcgcccaagc ggcatccaag
     3961 tgggcaaaat gcgcgtgccc ttgggggtgg tggggatcat ctatgagtcc cgccccaacg
     4021 tcactatcga tgcggcaagc ctgtgtttaa aaagcggcaa cgccgccatt ttgcgcggcg
     4081 gcagtgaagc cattgaatcc aaccaggcga ttgccgcctg tatcgcccaa ggcctgagcg
     4141 ccgtgggcct gcccgaaagc gtggtgcagg tggtcaacac caccgaccgc gctgccgtgg
     4201 gcgctttgat tagcatgccc gaatacgtgg atgtgatcgt ccctcgtggc ggccgctctt
     4261 taattgagcg cattagcctg gaggcgcggg tgccggtgat caaacactta gatggcatct
     4321 gccacgtcta tatagatgcc gaggccgact tcgaaaaatc ctttaatatc gccatgaacg
     4381 ccaagaccca ccgctacggt gtgtgcaatg ccatggaaac cctactggtc catcaattcg
     4441 cggccccaca aatactgccc cgcttagccg ctgccctgat tgaaaagggc gtggagctgc
     4501 gcggctgcgc caccacctgc acgttgatta acgccctggc ggccaccgaa gaagactggc
     4561 gcaccgagta cttagcgccg gtattgtcca ttaaggtggt ggagcatctg gatgaggcca
     4621 taagccacat taatacctac tcctccatgc acactgacag cattgttacg gagaactaca
     4681 ccctggctcg ccgctttttg cgggaggtgg attcggcgag tgtgatggtg aatgcctcca
     4741 cccgttttgc cgatggcttt gagtacggct taggcgccga gattggtatt tccaccgaca
     4801 aaatccacgc ccggggcccg gtaggtttag agggtttaac cagccaaaaa tggattgtgt
     4861 ttggcgacgg gcaagttcgc cagtaaatgg attgttaagt ggagcgcaca cagagcccgc
     4921 tgttggtata cggcggcagt ttcgacccgg tgcacaacgg ccatgtggcg gccttttggc
     4981 tggcggctca tctgctcaat gccggggagg cgcggctgtt gccgtgtttt ttgtcgccgc
     5041 ttaaaagcca ggtgtttacc acgcctgagc agcgctgcgc cctgctgcaa ttggtggtaa
     5101 ccggcttaaa ccagcgctca tccgcctgtt tgttcagcct cgatcggcgg gaaatcgaga
     5161 tcgatgggcc gagcttcacc tgtgacaccc tggccgcttt acgccaggag cagggtgaaa
     5221 cccggcctat tatctggctt ttgggggatg atgcctggcg gcagctgccg cagtggcgcc
     5281 attggcagcg cctcaccgaa tttgcccatc tgctgattgc cgcaaggcct ggggtagaag
     5341 ccccaataga acctgcccaa actgcttggg ctgcagcgcg ccagtgtgct ttggcgcagc
     5401 tgggtttaag ccccagtggg ggcatagccc atatgaataa tgctttgttg gctatctccg
     5461 ccagccacat tcgcacacag atccaacagg gcgtgcgccc gctggggctg ctgccggatg
     5521 cggtggaaga gcgcatttac cagcagggcc tgtatctcaa ctcttagtaa accaaaatcc
     5581 tatgaccgat cttaatgaag tagtaattac cgccctggaa aacctcaaag ggcaaaaaat
     5641 cgtcaacctc aacgtgcggg atctctccga cgtgaccgat tgtttaatca tcgccaccgg
     5701 cacctccagc cgccacgtga aatccctcgc ccacaatgtg gtggaagagg ccaaggaaaa
     5761 gggctttcgc cccttaggga ttgaaggcct gcaggaaggt gaatgggccc tggtggattt
     5821 tggcgatacg gtggtgcatg taatgtccgc cgaggcgcgg gaattttacg acttggaaaa
     5881 actctggagc aaagtgcccg ccaaccgcac gccggactcc aaagcctaaa catgcgggta
     5941 cagctggtgg ccgtgggcac caaaatgccc gcctgggtac aaagcggctt taccgagtac
     6001 gccaaacgcc tgccgaagga actcacccct aagctgctgg aattggaatt aggctctaaa
     6061 tccaaacccg gcaatatgga agcggctaaa aaagcagaag ctgaagccat tttggctgca
     6121 attccggcgg agcactttgt agtgatgctg gatgtgggcg gcaaagcctg gtccacggag
     6181 gaattagccg ctcagttggc ccagtggcag atgagcgggc gggattttag ttttgttatc
     6241 ggcgggccgg ac
//
LOCUS       DAOQGS010000209         6251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6209_length_6251_cov_5.069238, whole genome shotgun sequence.
ACCESSION   DAOQGS010000209 DAOQGS010000000
VERSION     DAOQGS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_6251_cov_5.069238"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PKZ52_09220"
     CDS             complement(<1..572)
                     /locus_tag="PKZ52_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HNG59995.1"
                     /translation="MISHHPDSNLLVEFANGSCDWALGILINTHLQHCRHCRSQVQQL
                     EQLGSALLTAGPKVSVDDACLEKLLRRIDETPATAAAVETKTSQTPGLPPVVQRLIKP
                     GARWQKISKHLKQQRLKTGQNKYEVALQSIKSGGQVSRHDHRGLEYTVVLQGSFSDAE
                     GIYCTGDFVLRQPGQAHKPTASQNEDCLCLT"
     gene            complement(569..1180)
                     /locus_tag="PKZ52_09225"
     CDS             complement(569..1180)
                     /locus_tag="PKZ52_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNG59996.1"
                     /translation="MTPDLTPSAPSPLVRSDHLSQLLIKVGNQDRSAFAELFRHYGPL
                     IKGFAIQHANSRFPIEAADELVQEVMLKVWLKAKSFEPSKASANTWVFTVMRNCRIDL
                     LRRSQRRYFESGDIDADDIWDDSDDHSPFTFLHQGRQQQQVAQGFAQLPLEQKQALTF
                     VYMHGKSHAEIAEETGLPLGTVKSRVRMGLKKLGSLFKPGEGL"
     gene            1327..1809
                     /locus_tag="PKZ52_09230"
     CDS             1327..1809
                     /locus_tag="PKZ52_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNG59997.1"
                     /translation="MSSHQAHLASPLLAPGELLEEFKRCYATFGIHTIAQMQKIYDPA
                     ITFVDPLHRIEGWPALERYFASSAANLTYCRFEFIDSVAGEGAAFFKWHMHYAHQKIA
                     KGKPLSLVGATQIKFGQRVTYHEDFYDLGAMVYQHIPIIGLGIQKLRHCMLRASRSGR
                     "
     gene            1799..2560
                     /locus_tag="PKZ52_09235"
     CDS             1799..2560
                     /locus_tag="PKZ52_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNG59998.1"
                     /translation="MEGKTLWIIGASAGIGAEVALQLAQAGHFVIVSARDEAALNQLS
                     FRAPTRIKCLPLDLAAPDTFAAVRQELLNITDFLDGIIFCAGVCEYETNLEFNYAQYS
                     KVMQVNFLGLIAVLNMAKPLLKKSTCRAQIGVVSSLATVVAFPRNEMYGASKAALDYF
                     VAALRCDTVHLPVDITWVRPGFVATRLTAKNDFAMPFLMDVPTAAQKIVRGFLNRKRT
                     VIFPYRLYVLLKLMAAFNPLWRSLCAKFLTRTKTW"
     gene            2554..3858
                     /locus_tag="PKZ52_09240"
     CDS             2554..3858
                     /locus_tag="PKZ52_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG59999.1"
                     /translation="MVEPFAQGTPAQQRIAVIGSGIAGMACAHYLSRVHEVCLFEGAS
                     RFGGHTATQTVHWQGVEYGVDMGFIVYNDWTYPNFIRLLTELNIASQPTVMGFSVTKG
                     QGAYEYAGANLNSLFAQRRNLLKPGHWCMLLDIVRFNKQATADFLAGRLSQTESLGAY
                     LQRLKFSAQFVDYYLAPMGSAIWSSTTAEVLNFSAYFFVRFFHNHGLLSIKQRPQWRT
                     LVGGSSVYIKPLLANVARSVKGTPVLRVERSESGARVFYGDQSELFDQVVFACHSDEA
                     LGLLGDASAAERDILGAITYRPNRVTLHRDTKLLPQRPLAWSSWNYLVQGAGAQPPLL
                     TYNMNILQNIQAPITFCVTLNDPGLIKPEMVFHEVTFSHPVFSAAATQAQERWQEING
                     VNKTWFAGAYWLNGFHEDGLVSALRVVNQLGVSVPILNNHRP"
     gene            3862..4659
                     /locus_tag="PKZ52_09245"
     CDS             3862..4659
                     /locus_tag="PKZ52_09245"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNG60000.1"
                     /translation="MTGSPDKPLSFAGAPISTAPKIYSGQLRHRRFWPKSHAFTYNVF
                     MLYGNCQDAAQMRIGAWLGPRFWHWLRFCRGDFHGDPNLDLDSAVRNTVFNTTGKTPT
                     GPIMFLANYRTLGISMNPLTTYYCWNEQQTQVEFIVAEVNNTPWNERHAYVLTCEREQ
                     KQDQSFQKVFQVSPFNPLNMRYRWVSTQPGAQLLVHIENWQALSGGEQKIMDATLTLA
                     ALDLTEKNLRKIILSAPVMSLKVIAAIYWQALRLWLKRVPFIGRAAA"
     gene            4734..6047
                     /locus_tag="PKZ52_09250"
     CDS             4734..6047
                     /locus_tag="PKZ52_09250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNG60001.1"
                     /translation="MSMMPDISPLAFVGPAQSQSKKINSLTARLKQPVARGWVDRLAR
                     NGVMRLFASLTRGTLILREGADVFEFGEPAAQASVVAEVDILHPSFYRFILFGGTVGS
                     GEAYMQGTWQSPDLTRVIEVMVMNLATLANMNKRWQRFASCIGAWRYYWQKNSKAGSK
                     RNISAHYDLSNDFFKLFLDPTLMYSAAIYPSENASLYEASLNKLQRVCDQLALKADDH
                     LLEIGTGWGGLAIYCAQTTGCKVTTVTLSQEQFNHAREQVAAAGLSGQVEVHLCDYRD
                     IQGQYDKLVSIEMIEAVGHQYFGNYFAKCSSLLKPHGKMLLQAITIPHERFERGKYKM
                     DFIRQYIFPGGCLPSQQVIMDNITRHTDMELVNFKDITLDYARTLNAWAEAFNSQHTA
                     VKALGFDDVFIRMWAFYLSYCEGGFRQRVIGTSQWLFAKPLAEVQ"
BASE COUNT         1441 a         1491 c         1626 g         1693 t
ORIGIN      
        1 gtgaggcata aacaatcttc attttggctg gcggtgggtt tgtgtgcttg gccgggttgg
       61 cgtaggacaa aatccccggt gcaataaata ccttcggcat cggaaaaact gccctgtaat
      121 accaccgtat attccaggcc gcggtggtcg tggcggctga cttggccgcc gcttttaatg
      181 gattgcaggg ccacttcgta tttgttctgg ccggttttta aacgctgctg ttttaaatgt
      241 ttggaaattt tttgccagcg ggcgccaggt tttattaagc gctgcacaac agggggcaag
      301 cccggtgttt ggctggtttt tgtctctact gctgccgctg ttgcaggtgt ttcatctatg
      361 cggcgcagga gtttttctaa gcaggcatca tcaacactga cttttggccc agcggttaat
      421 aatgcagagc ccagttgctc caattgctgc acttggctgc ggcagtggcg gcagtgttgt
      481 aggtgggtat taattaaaat acccaaggcc cagtcacaac tgccgttggc aaattccact
      541 aataaattac tgtcggggtg gtgggaaatc atagtccttc tcccggctta aataagctgc
      601 ctagtttttt caggcccatg cgcacgcggg attttacggt gccaaggggt aagccggttt
      661 cttcggcgat ttctgcgtgg gatttgccgt gcatatatac aaaggtgagc gcttgttttt
      721 gttcgagggg gagttgtgca aagccttggg caacttgttg ctgctggcgg ccttggtgta
      781 aaaaagtaaa tggtgaatga tcgtcgctgt cgtcccaaat atcgtctgcg tctatatcgc
      841 cgctttcaaa atagcggcgc tggctgcggc gcaataagtc gatgcggcag ttgcgcatta
      901 ccgtaaacac ccaagtattg gcggaggctt tgcttggttc aaaacttttg gctttcaacc
      961 atactttaag catgacctcc tgcaccaatt cgtcggcggc ctcgatggga aaccggctgt
     1021 tggcgtgttg gatggcaaaa cccttaataa gcgggccgta atggcgaaat aactcggcaa
     1081 atgcgctgcg atcctggttg ccaactttaa taagcagctg gcttaggtgg tcgctgcgca
     1141 ccaggggcga cggagctgat ggggttaaat caggagtcat gaaacaccaa ttgggttagg
     1201 ttgggtaagt gtacgccggg gtggtttgaa tggatcatag ttgcctaaaa atacctggcg
     1261 cctgcactca tccacctggc aggtcttagc gtacttaccc ttacacactc tgtggctgaa
     1321 caccctatgt cttctcatca agcgcattta gcatcccccc tgctggctcc tggcgagcta
     1381 ctggaggagt ttaagcgttg ttacgcaacc tttggcattc acaccattgc tcagatgcaa
     1441 aagatttacg acccggcaat aacctttgtg gacccgctgc atcgcattga ggggtggccc
     1501 gctttggagc gatattttgc ctccagtgcg gccaatttaa cctattgccg gtttgagttt
     1561 attgattccg tagcggggga gggggcagcg ttttttaaat ggcatatgca ttatgcccat
     1621 caaaaaattg ccaagggtaa accgctgagt ttagtaggcg ccacgcaaat taaattcggc
     1681 cagcgggtaa cctaccacga agacttttac gacctaggtg ctatggtgta ccagcacatt
     1741 cctattatcg gtttgggcat tcaaaaattg cggcactgta tgctgcgagc tagccgcagt
     1801 ggaaggtaaa accctttgga tcatcggtgc cagcgcaggt ataggggctg aagtggcact
     1861 gcaattagcc caggccgggc actttgtgat tgtgagcgcg cgggatgagg cggcgctaaa
     1921 tcaattgagt ttccgtgcgc ctacccgtat taaatgcctg ccgttggatt tggctgcccc
     1981 cgatactttt gccgcggtac ggcaggaatt attaaacatt acagattttc tagacggcat
     2041 tattttttgc gcgggcgtct gtgaatatga aaccaacctg gaatttaatt acgcccaata
     2101 cagcaaagtg atgcaggtga attttttagg tttgattgcc gtgctcaata tggctaaacc
     2161 cttattaaaa aaatccacct gccgcgccca aataggcgta gtgagcagct tagctacagt
     2221 ggtggccttc ccccgcaacg aaatgtacgg cgcctccaag gcggctttgg attattttgt
     2281 agcggcactg cgctgcgaca ctgtgcattt accagtggat attacctggg tgcggccggg
     2341 ttttgtggcc acacgcctaa cggcaaaaaa tgattttgcc atgccgtttt taatggatgt
     2401 gcccacggcg gcacagaaaa ttgtgcgtgg gtttttaaat cgcaagcgca cagttatttt
     2461 cccctatcgc ctgtatgtac tgcttaagct tatggcggcg tttaatcctc tctggcgcag
     2521 cctgtgcgca aaatttttga cgagaaccaa aacatggtag agccattcgc gcagggtacg
     2581 cccgcgcagc agcgcattgc tgttatcggc agcggtattg cgggcatggc ctgcgcccat
     2641 tatttaagcc gtgtgcatga ggtgtgttta ttcgaaggcg ctagccgttt tggcggccac
     2701 accgccaccc aaactgtgca ctggcagggg gtggaatatg gggtggatat ggggtttatt
     2761 gtctataacg actggaccta cccgaatttt attcgccttt taactgaatt aaatattgcc
     2821 agtcagccga ctgtcatggg ctttagcgtc actaaagggc agggcgctta tgaatacgca
     2881 ggtgctaatt taaatagttt gtttgctcag cggcgcaacc tattaaagcc cggccattgg
     2941 tgcatgctgc tggatatagt gcgctttaat aaacaggcta cggcggattt tttagccggg
     3001 cgcctatcgc aaacagagtc cctcggcgct tatttgcagc ggctaaaatt tagtgcacag
     3061 tttgttgatt attatctggc gcctatgggg tcggccattt ggtcctccac cactgctgaa
     3121 gtgctaaatt ttagcgccta tttttttgtg cgtttttttc acaaccacgg cttattaagt
     3181 attaaacagc gcccccaatg gcgcacttta gtgggcggct caagcgttta tattaaaccc
     3241 ttgctcgcca atgtggcacg cagcgttaag ggcactcccg tgttgcgggt ggagcgcagt
     3301 gaaagcggcg cgcgggtttt ttacggcgac caaagtgaat tattcgacca ggtggtattt
     3361 gcctgccaca gcgatgaagc cttgggtttg ttaggcgatg ccagtgccgc cgagcgggat
     3421 attttaggcg ctattaccta caggcctaac cgagtaaccc tgcaccggga tacaaagctt
     3481 ctgccgcagc gcccactggc ttggtcgagt tggaattatt tagtgcaagg ggcgggggct
     3541 cagccgccgc tgctcaccta taacatgaat attctgcaaa atattcaggc gcctattacg
     3601 ttttgcgtca cgctgaatga tccgggttta attaagcccg agatggtttt tcatgaagtg
     3661 acattttcac acccggtatt ttccgccgct gccacccaag cccaagagcg ttggcaggaa
     3721 attaacggcg taaacaaaac ctggtttgca ggcgcctatt ggttgaatgg ctttcatgag
     3781 gatggcctag taagtgcttt gcgggtggtt aatcaattgg gcgttagtgt gcctatttta
     3841 aataatcaca ggccctaatt tatgacgggc tccccggata agccactatc atttgcaggc
     3901 gcacctatta gcaccgcgcc caaaatttat tccggccaat tgcgtcaccg caggttttgg
     3961 ccaaaatccc atgcctttac ctataacgta tttatgcttt atggcaattg ccaagatgcc
     4021 gcgcagatgc gtatcggtgc ttggttgggg ccgcgttttt ggcattggct gcgtttttgt
     4081 cggggggatt ttcacggcga cccgaacctt gatttagaca gcgccgtgcg caacaccgta
     4141 tttaatacca cagggaaaac tccaaccggg ccgattatgt ttttggccaa ttaccgcact
     4201 ctgggcattt ccatgaaccc gctcaccacc tattattgtt ggaatgagca acaaactcag
     4261 gttgaattta ttgtcgcgga ggtgaataac accccctgga atgaacgcca cgcctacgtg
     4321 ctaacctgtg agcgagaaca aaaacaagat caatcttttc agaaagtttt tcaggtatcg
     4381 ccttttaatc ccttaaatat gcgctaccgt tgggtatcta cccagcctgg tgcgcaacta
     4441 ttggtgcata ttgaaaattg gcaggcacta agcggcggtg agcagaagat tatggatgca
     4501 accctcaccc ttgcggccct tgatctgacc gaaaaaaacc tgcgtaaaat aatattaagt
     4561 gcaccggtga tgagccttaa agtaattgcc gccatttatt ggcaagccct gcgattatgg
     4621 ttaaagcgtg tgccttttat cggccgcgcc gccgcataaa aaattaattg caaacctact
     4681 ggccggatta ttcccatacc gcgcccaaga gcagtaaacc ccaggagaca cccatgagta
     4741 tgatgccgga tatttcccct ttggcatttg ttggcccagc acagtcgcag agtaaaaaaa
     4801 taaatagcct tacggcgcgg ctgaaacaac cggtggcgcg tggctgggtg gatcggcttg
     4861 cccggaacgg tgtgatgcgt ttatttgcct cacttacgcg gggcacctta attttgcgcg
     4921 aaggcgctga tgtatttgaa tttggcgagc cagcagcaca agccagcgtg gtagccgaag
     4981 tggatattct gcatccatct ttttaccgct ttattttatt tggtggcaca gtgggctctg
     5041 gggaggctta tatgcagggc acctggcaga gccctgattt aacccgcgtg attgaagtga
     5101 tggtgatgaa ccttgctacc cttgccaata tgaataaacg ctggcagcgt tttgcctctt
     5161 gtattggcgc ctggcgttat tattggcaaa aaaattccaa ggcgggttcc aaacgcaata
     5221 tctccgccca ctacgattta agcaacgatt tttttaagct ctttttagac ccaaccctaa
     5281 tgtattctgc ggcgatttat ccatctgaaa acgcaagcct ttatgaggcg tccttaaata
     5341 aattacagcg ggtgtgtgat cagttggctt taaaggccga tgatcattta ctggaaattg
     5401 gtaccggctg gggtggcctc gctatttatt gcgcgcagac taccggctgc aaagtcacca
     5461 ctgtaacgct ttcccaggag caatttaacc acgccagaga gcaggtggca gcggcgggct
     5521 tgagcggtca ggtggaggtt catctgtgtg attatcgcga tattcaaggc cagtacgaca
     5581 aactggtgtc catcgaaatg attgaagcag ttggccacca atatttcggt aattattttg
     5641 ccaagtgctc cagcctgtta aaaccccatg gcaaaatgct gttgcaggcc attactatcc
     5701 cccacgagcg ttttgagcgc ggcaaatata aaatggattt tattcgccag tatattttcc
     5761 ccggcgggtg cctgccttcc caacaagtca ttatggacaa tatcactcgc catacggata
     5821 tggagttggt gaatttcaaa gacatcaccc tggattatgc ccgcacccta aacgcctggg
     5881 cggaggcatt taatagtcaa cacaccgctg ttaaagcctt aggctttgat gacgtgttta
     5941 tccgtatgtg ggcgttttat ttatcttatt gcgaaggcgg ctttcgccag cgggtgattg
     6001 gtacctcgca atggttattt gccaaaccat tggccgaggt gcagtaatgc tttatgagcc
     6061 ttgaattctt atacgagcaa atacgcggca atcgggtacg gtatcacttt acttatcctg
     6121 agccgcttag ggcaaaggcc taaggaacct ctgaaaaatt accttactcg ctctggcgtc
     6181 gttgaatttg ccaaaaaaat actcatttac tgcgtgtaaa ctcggcaatt gctcctgcat
     6241 tgctctacct c
//
LOCUS       DAOQGS010000210         6250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6210_length_6250_cov_5.637127, whole genome shotgun sequence.
ACCESSION   DAOQGS010000210 DAOQGS010000000
VERSION     DAOQGS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6210_length_6250_cov_5.637127"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            635..1282
                     /locus_tag="PKZ52_09255"
     CDS             635..1282
                     /locus_tag="PKZ52_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNG60002.1"
                     /translation="MNSLIQQYMQRLKKKLAIRPSPQLLVHAIELFHGATEQLTQIAK
                     QTRHVRFACQKGCAHCCSLRVEVLPPEAFFISNHIKTWPQAQQASLIKRLEQHVLYAD
                     ERSFVQYNRPCPFLTANGACSIYAVRPHKCRANLSLNVQPCIETGDGQEDTALKSAHG
                     QLAIQTILFYKHKKLVMNPAELGASVLAALTNSGLQQEWAQGVQVFKLLPEKIQF"
     gene            complement(1376..2671)
                     /locus_tag="PKZ52_09260"
     CDS             complement(1376..2671)
                     /locus_tag="PKZ52_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60003.1"
                     /translation="MIKTAHRLSIAVLSVLSTACVAPNDHPPSSSSSSSSSSGSSSSS
                     SSGAPGAYLSVKGGYFTRTPAGTSRYSTLNGLSNLIIRDNSSEIEIHVQGLTANAQYK
                     SVLTRGTCFSIDSNATYRQGLGGNEITPTISVDAFGMGMAKDAQNFALNDTDLSLAIK
                     DLGNENTIACADLHSSAGIKGMVRMGKSYYYNWVGYVSANDNGHSRAEINPSTGFRRI
                     DTSGPVDAYANMSNCNSEPMEYGQSKGGHGAENSLWPHAEETEAFLGIWASNPFKVRL
                     NEIKSLVFSEQQSQQPLPYRCIDLDTKLLTFRSGSFKPTDAGVALYGTLAGRAILETS
                     LEGNTTASITMAGLKPNTTYSNHVHNGLCADAGGNHYLHDIHGADNAVNGMFPVFTTD
                     ATGYVHYQLSANKIVRPDARSIVIHEPGSGVKIACADLD"
     gene            complement(3045..3542)
                     /locus_tag="PKZ52_09265"
     CDS             complement(3045..3542)
                     /locus_tag="PKZ52_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNG60004.1"
                     /translation="MSNTSASLSPAPLSAAENVSALTPQDAYLVVAFASHEGDIVDEH
                     FGSAQAFYVYDIHPERAELIGSIQFAREAKDGNEDKLKPKLAWLTGSDVVYCASIGGS
                     ATRQLIAMGITPMKVSEGPDISELIEGIQAQLSGSMEFWLANILKQKQKSAGTDRFSV
                     DEEWG"
     gene            3541..3765
                     /locus_tag="PKZ52_09270"
     CDS             3541..3765
                     /locus_tag="PKZ52_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60005.1"
                     /translation="MASSRGLRISARRQQKVVHGAFAKNSEFLAFFGVRPATFSCGTA
                     VETPQIVRFATKYPSLGGFVSRLFIAGFGV"
     gene            4660..5595
                     /locus_tag="PKZ52_09275"
                     /pseudo
     CDS             4660..5595
                     /locus_tag="PKZ52_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
     gene            complement(5939..>6250)
                     /locus_tag="PKZ52_09280"
     CDS             complement(5939..>6250)
                     /locus_tag="PKZ52_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60006.1"
                     /translation="LASADSCEAILKKAQEEQALIPGYQAMHTISGKTRAYFHSAPAN
                     TCINKQLFLVPGDSVVAYADYQEFTSVLYIHPKTGVETEGWVLTQRLKATGTGIAPQQ
                     H"
BASE COUNT         1582 a         1452 c         1501 g         1715 t
ORIGIN      
        1 gaacctctga aaaattacct cactcgccct agccacgttg aatttggcaa aaaaaatgct
       61 catttactgc gtgtaaactc ggcaattgct cctgcattgc tctacctcct gcctccctgc
      121 agtcgtccgc tttttttgcc aaattcgcct agctattgct tcgccgccga cattattcag
      181 aggttcctta gtttgcattt gtgctgtatg gagccgcaca gttaaaggcg gcatgagcag
      241 cctgctgaaa taaggcgaca gtttggcaag ctatattgag tgtaaccatg ctgttttttt
      301 acggtaattg aacgtgatac ctaggttgta tagcacctat gagctttcta gcacttatcg
      361 gttgagtgct acgctacctg cagccatgca tgcttagccc gcataatgag cagttataat
      421 ttgcttaaaa acccgtaaaa ctgcatcctt accatcgccc gcgcgaaaac gccaggtgat
      481 aatcacgaat gctgaatttc cgtaaaggcc tttaaatcat tctggaggtg ccttttaatg
      541 tttctttaag taatagctgt gaacaagcgc tgcctgccat taaatggctc gccagtttag
      601 tagttgtaac ataaatttta aaacacgaga tgcgatgaac agtttaattc agcaatacat
      661 gcagcgactt aagaaaaaac tcgccattag gccgagcccg cagctcttgg tgcacgccat
      721 cgagttattc catggggcga cggagcaact tacgcaaatt gccaaacaaa cccgccatgt
      781 tcgctttgcc tgccaaaaag gttgcgccca ttgttgttct ttgcgggtgg aggtattgcc
      841 gcccgaggca ttttttatat ccaaccacat aaaaacctgg ccccaggcgc agcaggcgag
      901 tttaatcaaa aggctagagc agcatgtgct ttatgcggat gagcgcagct ttgtacaata
      961 caaccggccc tgcccatttt taacagcaaa tggcgcctgc agtatttatg ctgttcgacc
     1021 ccacaaatgt cgggctaatc tctcgcttaa tgtgcaaccc tgtatcgaga caggtgatgg
     1081 gcaagaagat accgccctta aatccgccca tggccaattg gcgatacaaa ccattttgtt
     1141 ctacaaacat aaaaaattgg taatgaaccc tgcggaatta ggtgccagcg ttttggccgc
     1201 cctcaccaac tcagggttgc aacaagaatg ggcgcagggc gttcaagtat ttaaattgct
     1261 gcctgaaaaa attcaatttt aagctggccc tggcggcatt aattgccaat gtgactatag
     1321 ggttgccaat tgtgaggtcg cggctgaaaa gcacatccct gtgttagttg attaattaat
     1381 ccaagtccgc acaggcgatt ttgacccctg accccggctc atggatcaca atgctgcgtg
     1441 catctgggcg gacaatttta ttggcggata attgatagtg tacatagcct gttgcatctg
     1501 tggtaaatac cgggaacata ccattcactg cattatcagc gccgtggata tcatgtaaat
     1561 aatggttgcc gcccgcatcc gcgcacaagc cgttatgcac atggttggag taagttgtgt
     1621 tgggttttaa gccggccata gtaatacttg cagtagtatt gccctccagg ctggtttcta
     1681 aaatagcgcg gcccgctagg gtgccataga gcgcaacccc tgcatctgtg ggtttaaagc
     1741 tgccacttct gaaggttaat aatttagtat ctaaatcgat acagcggtaa ggcaacggct
     1801 gttgggattg ctgctcagag aagactaaac ttttaatttc gtttaaacgc actttaaatg
     1861 gattgctggc ccaaatacct aaaaatgcct ctgtttcttc cgcgtggggc cataggctat
     1921 tttcagcgcc atgcccgccc tttgattgac cgtactccat cggttcggag ttgcaattac
     1981 tcatattggc ataggcatcc accggcccgc ttgtatcaat tcgacgaaag ccagtgctgg
     2041 ggtttatttc cgctctggaa tggccattgt cattggcgct aacataaccc acccaattgt
     2101 aataatagga tttgcccatt cgcaccatgc ctttaattcc ggcgctggag tgtaaatcgg
     2161 cacaggcaat ggtgttttca ttgcctaaat ccttaatggc caaacttaaa tctgtgtcat
     2221 tcagcgcaaa attctgcgcg tcttttgcca tgcccatacc aaaggcatcc acgctaatag
     2281 taggtgtaat ttcattgccg ccgagcccct gtctataggt cgcattgcta tctatgctaa
     2341 agcaggtgcc gcgggttaac acggatttat actgggcatt ggccgttagg ccctgcacat
     2401 gtatttctat ttctgagcta ttgtcacgaa taattaaatt gctcagccca tttaaagtgc
     2461 tatagcggct agtgcctgct ggcgtgcggg taaaataacc gccttttaca cttagataag
     2521 cgcccggcgc gccgctgctt gaactggaac tagagccgct gctgctggaa gagcttgaac
     2581 tgctgctggg gggatgatcg ttgggcgcca cgcaggcggt agataatacc gataagacgg
     2641 cgatggataa tcgatgagct gtttttatca tggttaaata ctctaaaaat tcctgtgaaa
     2701 gaaaataccc gtttgttaaa tttgttacac ctaatgtgta cgcggtaaat ttttaccttg
     2761 aagaaccaat tgggccgagc gtgataagtg cagggcgaat atgcaccgac ttatggggct
     2821 ggggcaccgg ccttagctag gcgtaggtac ataactcggc cttagacaaa gacacagagg
     2881 ggccttgccc cctccatatt tggtaggcga aaattgtatt tcaaaggaat attgccaacc
     2941 ggtgcacaaa cttcacccgt tcagacttaa tcagagcagc ttcaatgcat cattgccccc
     3001 aacaaaaaag gcgccctagg gcgccttttt tgttggggta atttttaacc ccattcttca
     3061 tcgacagaaa accgatccgt tccagcagac ttttgttttt gcttgagaat attcgccaac
     3121 caaaattcca tagagccact taactgcgcc tgaattccct caattaattc actaatgtct
     3181 ggcccttcac tgaccttcat aggcgtaata cccatggcga ttaattgccg agtggcggag
     3241 ccgccgatgg acgcacaata aaccacgtcg ctgccggtga gccaggcgag tttgggtttt
     3301 aatttatcct cattgccgtc tttggcttcc cgtgcaaatt gaatgctgcc aattaattca
     3361 gctctttctg ggtgaatgtc atacacataa aacgcctgtg ccgagccaaa atgttcatcc
     3421 actatatcgc cctcgtggga ggcaaaggcc acaactaaat aagcgtcttg gggcgttaaa
     3481 gcgcttacat tttcagctgc tgataggggg gcagggctta atgatgcaga ggtattcgac
     3541 atggcaagct ccagagggct gaggatttct gcaagacggc agcaaaaagt agtccacggc
     3601 gcctttgcta aaaatagcga atttttagcg tttttcggcg tgcgcccagc gacgttcagt
     3661 tgtggtactg cggttgaaac cccgcagatt gttaggtttg caacaaaata tccaagcctg
     3721 ggagggtttg taagtcgatt atttatagct ggttttgggg tttgaagcac atgatgagtt
     3781 tgtttggaaa gcggttgtta aaaccactat tgatatgaga tgcagctttt tattattaac
     3841 ccggcgacat ttagtgccgg gttaataatg gcggcatgga tgccgcactc ggcggtgcaa
     3901 accgccgcga gctgcgctaa aactagaaaa tccgcgcatt ttcgtaggtt tagcgcagcg
     3961 aaggataaat agatcaggac gatctattta tccgccgggt taataagagg cattaaatct
     4021 gaagagccat aggttaatta gggctgcagg ctgaatcgat tttcgctttg tatcagcaca
     4081 agcgtgagcc ttctaatgtc atttttacta ttaggcagat aattaagtgt tatgtgtccc
     4141 agcacacgaa ataccccccg ggctgggtca cagaacgggc ggaatattca tggtattgta
     4201 agataatcct agaaaaccct agttggagtt cagctgcgtt tttttggaga atacttttgc
     4261 tgtggtttct tgcaagtatt taaaaccatg aatacaaatc cttctgcggg ccggttgcgt
     4321 tggtgctcgc tgattgttct gtatcagggt tttaataaaa acaattttaa aaataagctc
     4381 ggagtattcg cctgaactgt ggcccttagc cctggctttc cggcgggcgg attgttcgga
     4441 accttttctc tacttaggca tgcaatgcat atgattacat caaataaaaa gccgtttgca
     4501 gcggcaattc agctctcggt tgtcacctgt gcgctggtgt tagccggttg taccggtggc
     4561 ggttctagct ctagctcttc cagcagctcc tcttcatcta gctcgagttc aagctctagc
     4621 tcgtcaagtt catccagctc aagctctagc ggccgcacca gttcaagctc tagctccagc
     4681 agttcaagtt ccagctcaag ttcttcctcc agctcaagca gctcaggttc tgccgcgaag
     4741 aaattcgtcg gtaatatcac cacgagtggt gctgtgcgtt cagactttat gaagtactgg
     4801 aaccaaatta cccctgagaa tgaaggcaaa tggggtgagg ttgaaaaaac gcgcgaccaa
     4861 tacaactggt cggctttaga caatatatat aagtttgccc gtcaaaataa tatccccgtc
     4921 aaagcccaca actttgtttg gggggcgcaa gcgccgagtt gggtgggcaa cctttccgcc
     4981 gccgaaatcc gtgaagaaat tgaagaatgg atgcgcgatt attgcgctcg ctacccagac
     5041 acctacgcaa ttgatgtgtt gaacgaggct attgacggcc acgcccctgc tcaatatgca
     5101 attaaagcct ttggttctac ctgggtgact gacgtattca gactggcgcg caaacattgc
     5161 cccaatgctg aattaattta caacgattac aatttcttaa cctgggatac ggataaaatt
     5221 attgccctaa ttacccccgc cattcaggcc ggttacgtgg atggcttggg tatgcaagcg
     5281 cacagcttgt acgatcccaa agtttggtct gcgcaggaaa ttaaagacaa gctggaccgc
     5341 atcggcgctt taggccctga cttgtatatc tctgagtacg acattgaagc cactgacgac
     5401 aataaacagc ttgaatacat gaaaatgcac ttccctgtgt tctggaacca ccctaaagtc
     5461 aaaggcatta cgctgtgggg ctatgtagtg ggcagaacct ggagaaccgg tacaggctta
     5521 attcaagaca acggtacccc gcgccctgct atgacttggt tgatggacta cttaaaaacc
     5581 gagcgcgcta agtaagcgca ggtttaaccc cggtattttt actggggtta aatttattta
     5641 aaaaggcgca gctactgcgc ctttttttcg tctgttattt tttaaggctt agattcagct
     5701 atagattgaa tttttgcagc tagatttctc agtcaataca gcttttaata cgagggctgc
     5761 cgaatttaag ttcactttgt tttaggttgg atgtttgccc tgcgccggga gttgttttgc
     5821 ctgtttgccc ttaagcacac ggcttaggaa taaagtttaa aattttagag gggggtgcga
     5881 ggcgtggatg tctggagtga cttgtttgag caccgaggga tgcgtgaggc atattagatt
     5941 aatgttgttg cggtgctatg cctgtgccgg tggcctttaa gcgttgggtt aaaacccagc
     6001 cctcagtttc tacgccagtt ttggggtgaa tataaagcac ggatgtgaac tcctggtaat
     6061 ccgcataggc aaccaccgag tcgcccggca ctaaaaataa ttgtttgtta atacacgtat
     6121 tggctggcgc gctatggaaa taggcgcggg ttttaccgct gattgtatgc atggcttgat
     6181 agccaggaat taaagcttgc tcctcttgcg cctttttcaa aatggcttcg caggagtccg
     6241 cagaggctaa
//
LOCUS       DAOQGS010000211         6250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6211_length_6250_cov_4.862793, whole genome shotgun sequence.
ACCESSION   DAOQGS010000211 DAOQGS010000000
VERSION     DAOQGS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6211_length_6250_cov_4.862793"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKZ52_09285"
     CDS             <1..763
                     /locus_tag="PKZ52_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60007.1"
                     /translation="LTASRAKLKEVTAATKGVKTTAAKARLAKAKAAVEAARNDYDTL
                     SSAYSSAKEGGEVKVKRAGRPPKAKATKGKPGPKPKAAAPAKRGPKPKAAAKVVKSSI
                     EAPKKRGRKPKAAAVVEAPKKRGPKPKAAKVEVKEAPKKRGPKPKAAKAASGEAPKKR
                     GPKPKNAGAAAAKVAKSTGEAPKKRGRKPKALVASETVEHEILDVITTEDAAEEITTA
                     PEAVEAEETLVESASEVETGHADEDEDQSSSLFDE"
     gene            complement(957..1457)
                     /locus_tag="PKZ52_09290"
     CDS             complement(957..1457)
                     /locus_tag="PKZ52_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HNG60008.1"
                     /translation="MNREKLVDFACRIYACASIERHCRYLQDDAFANVNLVLWLAWLQ
                     DQAIYLEKEALFQAEDLIGEISGEVVALLRHVRQRMKQIGHFTRVQEQLIAKHILQAE
                     LSVEKILLERLQDLTGRLARIQDPACEPLFLRDYFAHLQMPMPVAEEIKFFDLLDQEL
                     QLLSVD"
     gene            complement(1609..2673)
                     /locus_tag="PKZ52_09295"
     CDS             complement(1609..2673)
                     /locus_tag="PKZ52_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNG60009.1"
                     /translation="MNKPNILRAAILAAVLSPAFAMAQGVETYLSVPFNKYDSLDNTN
                     LDMSDSVGGALTAGYRFDNPFAVELTYGRADSGIRQNQQIDIDVQSLSLDGLYHFAES
                     DDLSPFVLLGVSEYQQDWDYAGVSSKNAQTAVDLGIGLKKFVTDHFVIRTEAKVFHTL
                     DYSNTHSALSVGFGYSFGDKAAPAPAPAPAPVAPKAPLDSDHDGVTDDADKCPGTPEK
                     LKVDAVGCPIILKENVNVTLQVLFDTNSDVVKPEYESEVKKVAEFLEQYQGTTAEIQG
                     HTDSRGSNAFNKTLSQKRADAIVKVLVEKFGVSAARLKAVGYGEEQLIVKNDKTPAEQ
                     AQNRRVIGKISAVKETEATK"
     gene            complement(2805..3491)
                     /gene="trmB"
                     /locus_tag="PKZ52_09300"
     CDS             complement(2805..3491)
                     /gene="trmB"
                     /locus_tag="PKZ52_09300"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNG60010.1"
                     /translation="MKPEFNPKAIRSFVIRAGRMTEGQKAAFDTWWPHYGVSLFAGPF
                     DRFAHFNNHQPLIVEMGFGMGDSLHAMALAAPDNNFVGIEVHPPGVGRLINNAGKDGL
                     KNLKVYMADATDVLRDCFADNSIDRFQLFFPDPWHKKKHHKRRVVQIEFLQLVQNKLR
                     QGGVLHMATDWQPYAEHMLEQVVQSGGWQNLGAPNGYADRPDYRPITKFEKRGENLGH
                     GVWDILVAKI"
     gene            complement(3487..4221)
                     /locus_tag="PKZ52_09305"
     CDS             complement(3487..4221)
                     /locus_tag="PKZ52_09305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNG60011.1"
                     /translation="MEIIQLQDLTIPIVRTRKRKTVTIKINKAQAQVHAPMGVARSFI
                     QEFVTSKARWIHKHVAAHQQRAQNSAAAAIKLHENTSIRLLGEQLWLRLVSHLPSPVE
                     RSGDSLLIRIQPHLPTEQALFLGVRAYVLQHGAHYCGALAARYCEQLGVYPKRISIKE
                     YRSRWGSCNARKEVQFNWLIALAPAKAIEYVVVHELCHLIHFNHSPAFWAAVGQLMPD
                     YAVWRRWFTSYGDVLWNVLDLKQYVN"
     gene            complement(4228..5349)
                     /locus_tag="PKZ52_09310"
     CDS             complement(4228..5349)
                     /locus_tag="PKZ52_09310"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNG60012.1"
                     /translation="MRLSERFTGTVRGLTSEGSGVVDHPSGRAVFVPGAWPGEQVEAK
                     ITQLKSQFALGELLSIIIPHPKRRPAPCPYHGQSAQHCGGCPWQFMAYDEQLAAKQQR
                     VEQAAARLNPALQVLPIQASPTELAYRNRAQLKSQGQQLGFVAAASHSLVAVEQCLLL
                     TPECAAQMQAVRAQLAPEPVKAKTPWLTWDINEREYRANSRLPFAQGNSAQNTYMRQW
                     LREQLQGCERDQPLLELFAGSGNFTQVIAAAGFTSIHAVEVVAEAVAALNKQSLPGVT
                     AQATDLFKDGVLNTLIKQVKPATVVLDPPRDGLKTIAQLQAKKTPIKTLLYISCDLAT
                     FARDAQMLIKEGFNLTLLQPVDMFPQTPHVELLARFARR"
     gene            complement(5718..>6250)
                     /locus_tag="PKZ52_09315"
     CDS             complement(5718..>6250)
                     /locus_tag="PKZ52_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reductase"
                     /protein_id="HNG60013.1"
                     /translation="CYLADRAGEDDIPIYLQGNKHFKLPKDQTLPVIMIGPGTGVAPF
                     RGFLQERQALGAAGKNWLFFGEQQQAQDYYFAAELQQIKSNGYLHKLSLAFSRDQAEK
                     IYVQHRIAEAAAELWRWLNEGAHIYVCGDASRMAKDVDAALTQVIASQGAMSIERAQE
                     ELRALADQKRYLRDVY"
BASE COUNT         1591 a         1622 c         1599 g         1438 t
ORIGIN      
        1 attaaccgcc agccgcgcca aactcaaaga agtgaccgcc gcaaccaaag gcgtgaaaac
       61 cactgccgct aaagctcgcc tagccaaagc caaagcagct gtagaagcag cccgcaacga
      121 ctacgacacc ttaagcagcg cctactcctc cgccaaagaa ggcggcgaag taaaggtgaa
      181 acgcgctggc cgcccgccta aagccaaggc aaccaagggc aaaccaggcc caaaacctaa
      241 ggcagcggct ccagcaaaac gcggccctaa acctaaagca gccgcaaagg ttgtgaaatc
      301 cagcatcgag gccccgaaaa aacgcggccg taagcctaaa gccgccgcag tggttgaggc
      361 gcccaaaaag cgtggcccca aacccaaagc cgccaaagtg gaagttaaag aagcacctaa
      421 aaaacgcggc cccaaaccca aagccgcaaa agcagccagc ggcgaagcgc ccaaaaaacg
      481 cggacctaaa cccaaaaatg caggcgcagc agcggcaaaa gtagccaaat ctactggcga
      541 agctcctaaa aaacgtggcc gcaaacctaa agcattagtg gcgagcgaaa ctgttgaaca
      601 cgaaatttta gatgtcatca ccactgaaga tgcggccgaa gaaataacca ctgcgcccga
      661 agcggttgaa gcagaggaga cacttgttga atccgcgtca gaagtggaaa ctgggcacgc
      721 cgacgaagat gaagatcagt caagcagtct gtttgatgaa taactagccg cgaaacaaca
      781 tcccagtttt gaggtgatgc cgctcaaaaa gcggcgccac aaaaaagccc ctttaaaagg
      841 ggcttttttg tatatgttat atgaacccaa ccgaaaaagc gcctacaccg cctagcgtga
      901 aaatacttcc attgaattca atagctatca gcctgtcctg tgggaagcat agagtcttaa
      961 tctacagaca gcagctgtaa ttcctggtcg agcaaatcaa aaaatttaat ttcctcggct
     1021 acaggcatag gcatttgcaa gtgcgcgaaa tagtcgcgta aaaacagcgg ctcacaggcc
     1081 gggtcttgaa tacgcgccaa gcgcccagtt aaatcctgca ggcgctccaa gagtattttc
     1141 tctacactca attcggcctg aagtatgtgc ttagcaatta attgttcttg cacgcgagtg
     1201 aaatgtccga tctgtttcat gcgctggcgc acatgccgca gcagcgcaac cacctcccca
     1261 gaaatttccc ctatgaggtc ttcagcttga aagagcgcct ctttctccag gtaaattgcc
     1321 tgatcctgca accaagccaa ccacaagact aaattgacat tggcaaaggc gtcgtcttgc
     1381 aggtagcggc agtggcgctc aatgctggca caggcgtata tacggcaggc gaaatcaact
     1441 aatttttcac ggttcatagg tacccaactt atttttatat tagggttaca accaagggcg
     1501 aacgcctcat tacaggcagc cactacaaac ttagccgcca ccggcagttt tacaagcgcg
     1561 ggcaataaaa aagggcacct aagtgccctt tagagagaaa agactcgttt atttagtcgc
     1621 ttcggtctct tttactgcac tgattttgcc gatcacacgg cggttttgcg cttgctctgc
     1681 tggagtttta tcgtttttca cgatcaactg ctcttcgcca tagcctaccg cttttaagcg
     1741 cgcggccgat acgccgaact tctcaactaa tactttcaca atggcgtcag cgcgtttttg
     1801 tgacagagtt ttgttgaaag cgtttgaccc gcgagaatcg gtgtggcctt gaatctctgc
     1861 cgttgtaccc tggtactgct ccaagaattc agcgactttt ttcacttcag actcatactc
     1921 gggcttaacg acatcgctgt tggtatcgaa caacacttgc aaagtaacat tgacgttttc
     1981 tttcaagatg atggggcaac ccacggcgtc cactttcaac ttctctgggg tgcctgggca
     2041 cttgtcggca tcatcagtca cgccgtcatg gtcggaatcc agtggtgcct taggagccac
     2101 aggggctgga gcgggcgctg gggccggagc ggctttgtcg ccaaaggagt aaccaaagcc
     2161 aacggacaag gctgagtgag tattagagta atccaaggta tgaaacacct tggcttccgt
     2221 gcggatgacg aaatggtcag tgacgaactt tttcaaacca atacccaggt caaccgctgt
     2281 ctgggcattt ttagacgata cgccagcata gtcccaatcc tgctgatatt cagacacgcc
     2341 caacaacaca aacggagaca ggtcatcgga ttcagcgaag tggtataaac cgtccaggct
     2401 taacgactgc acatcaatgt ctatttgttg attttggcgg atgcccgaat cggcgcggcc
     2461 gtaggtcaat tccacggcaa atggattgtc aaagcgatat ccagcggtta aagcgccgcc
     2521 cactgagtcg ctcatatcta agttggtgtt atccaaacta tcgtatttat tgaatggcac
     2581 gcttaagtaa gtctcaacgc cctgtgccat tgcaaaagca ggtgataata cggcagcaag
     2641 aatagcggcg cgcagaatat taggcttgtt catagccaac tcctcgtaga taagtattta
     2701 agttaatttg agaattatta ttagaatggt gccaaaagcg gctggggtag gtaattgcag
     2761 caattagatc attttcatag attaagcgca acctcaggta ttttttagat ttttgccacc
     2821 aaaatgtccc acacgccatg gccaagattt tcaccgcgtt tttcaaactt agtaataggg
     2881 cgataatccg gccggtctgc gtagccattt ggcgcaccca aattctgcca acctccagat
     2941 tgcaccacct gctccagcat atgttctgca tagggctgcc aatctgttgc catatgtaat
     3001 acaccccctt gccttaattt attctgaacc aactgcagaa attcaatttg caccacgcgg
     3061 cgcttgtggt gtttcttctt atgccagggg tcggggaaaa acaattgaaa acgatcaatg
     3121 ctgttatcgg cgaagcaatc gcgcagcaca tcggtggcat cagccatata aaccttaaga
     3181 ttttttaagc catcttttcc agcattatta attaagcgcc ccacacccgg tggatgcact
     3241 tcgatgccga caaaattatt gtccggtgcg gcaagcgcca tggcgtgcag ggaatcgccc
     3301 ataccaaacc ccatttccac aattaacggc tggtgattat taaaatgggc gaagcggtca
     3361 aagggacctg caaataaact gacgccgtaa tggggccacc aggtatcaaa agccgccttt
     3421 tgaccttccg tcatacggcc tgcgcgaata acaaaactgc gaatggcttt gggattaaat
     3481 tcgggtttaa ttaacatact gttttaagtc caacacattc cacaacacat cgccataact
     3541 ggtaaaccag cggcgccaaa cggcatagtc gggcattaat tgccctacgg cggcccaaaa
     3601 tgcgggggag tgattaaagt gaattaaatg acacaattcg tgcacgacta catattcaat
     3661 tgctttagca ggcgccaggg caatcagcca attaaattgc acctctttgc gcgcattaca
     3721 actgccccag cgggagcgat attctttaat actgatacgc ttagggtaga cacctaattg
     3781 ctcacaataa cgggcggcca gggcaccgca ataatgcgcc ccgtgctgaa gcacataagc
     3841 gcgcacacct aaaaataacg cctgttcggt gggcagatgg ggctggatgc gaattaataa
     3901 actatcgcca ctgcgctcaa ccggtgatgg taaatggctg actaaccgca accataattg
     3961 ctcacccaat aggcgtatgg aggtgttttc gtgcagctta attgcagcgg cggcgctatt
     4021 ttgcgcccgc tgttggtggg ccgccacatg tttgtgtatc caacgggctt tactggtgac
     4081 aaattcttga ataaaactgc gggcaacgcc cataggcgca tgcacctgag cctgggcttt
     4141 attaatttta atggtaacgg ttttgcgctt gcgggtacgc acaatgggaa tggttaaatc
     4201 ctgcaactga ataatttcca ccttaaatca cctacgcgca aagcgcgcta ataattccac
     4261 gtggggggtt tgcggaaaca tatccacagg ctgcaatagg gttaaattaa acccttcttt
     4321 tatcaacatc tgcgcatccc gggcaaaggt ggcgaggtca caggaaatat acagcagggt
     4381 ttttatcggt gtttttttcg cttgtaactg cgcaatagtt ttaaggccat cccgaggcgg
     4441 gtccaatacc acagtggccg gttttacctg tttaatcagt gtgtttaata caccgtcttt
     4501 aaataaatcc gtcgcctggg ccgtcacgcc cggaaggctc tgtttattaa gggcggccac
     4561 tgcttcggcg acgacttcta ccgcgtggat tgaggtgaag ccagctgcgg cgattacctg
     4621 ggtgaaattg ccggagcctg cgaataattc cagtaggggt tgatcgcgct cgcagccttg
     4681 caactgctcc ctgagccatt ggcgcatata ggtattttgc gcgctgttgc cctgggcaaa
     4741 gggtaagcgg ctgttggcgc ggtattcgcg ctcgttaata tcccaggtga gccaaggagt
     4801 tttggccttg accggctccg gtgctaactg ggcgcgcacg gcctgcattt gggctgcgca
     4861 ttcaggtgtg agcagtaaac attgctccac ggccaccaag gagtggctgg ccgccgccac
     4921 aaaacccagc tgctgcccct gggatttaag ctgcgcccgg ttgcggtagg ccaattcagt
     4981 gggggaggct tggatgggta acacctgcaa agcagggttt aggcgcgccg ccgcctgctc
     5041 tacccgctgt tgtttggcgg cgagctgctc atcataggcc ataaattgcc aagggcagcc
     5101 gccgcagtgc tgggcgctct ggccgtggta ggggcaaggg gccgggcggc gcttgggatg
     5161 gggaataatg atgctgagca gttcgcccag ggcaaactgg cttttgagtt gggtaatttt
     5221 ggcttccacc tgctcgccag gccaggcacc tggcacaaac accgcccgcc cgctggggtg
     5281 atcgaccacg ccactgcctt cgctggtgag gccgcgcacg gtaccggtga atcgctcgga
     5341 taatcgcata aacaggctgg ggaaattttg ggcgggggat tctaccgaaa atataccgcg
     5401 ctggcttggg tttctggttg gggattgcgc aggcctatgg tggggctgcg ggcttagttg
     5461 ttatggttga ggcctgggct ctgaataacc gctgttgggt tgaagggcaa cggcctttaa
     5521 cccaattaac ccctccccag ccctcccctt gtaaggggag ggagccgaaa cgcctttatc
     5581 attggcaaac tatgtgggtt tgaaggttta tttgctcccc cctccttaaa ggcgcaagtg
     5641 cctctattac aagcacaatg cctagggaat aggtttttta ttggtgagtt aatgcggcaa
     5701 tccgcaggct aagaggccta atacacatcc cgcagataac gcttttgatc ggccagggcg
     5761 cgcagttctt cttgggcgcg ctcgatactc atggcgcctt ggctggcgat cacctgggtg
     5821 agggcggcgt ctacgtcttt ggccatgcgg ctggcatcgc cgcagacata aatatgcgcg
     5881 ccttcgttga gccagcgcca aagctcggcg gctgcttcgg cgatgcggtg ttgcacgtag
     5941 attttttcgg cttgatcgcg agaaaaggcc aggctcaatt tgtgtaaata gccgttggat
     6001 ttaatctgct gcaattcagc ggcaaaataa taatcctggg cctgttgctg ctcgccgaaa
     6061 aataaccagt ttttacccgc agcccccaga gcttggcgct cttgtaaaaa gccgcgaaag
     6121 ggcgcaacgc ccgtgccggg gccgatcata atcaccggca gggtttgatc cttgggtaat
     6181 ttaaagtgtt tattcccctg cagataaata ggtatgtcat cctcccctgc cctatcggct
     6241 aaataacagg
//
LOCUS       DAOQGS010000212         6238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6234_length_6238_cov_3.757561, whole genome shotgun sequence.
ACCESSION   DAOQGS010000212 DAOQGS010000000
VERSION     DAOQGS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_6238_cov_3.757561"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..979
                     /locus_tag="PKZ52_09320"
     CDS             161..979
                     /locus_tag="PKZ52_09320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG60014.1"
                     /translation="MQKTLSTIEPFTPQWLDVREPADKAARDKGLQAMFIDWLNTHNT
                     PPKILDLGAGTGANLRALYKPNQSWRLVDWDNTLLNEALRRHPQYYVTTHKANLNALE
                     SLPWDGVTAVTASALFDLTSAQFIDQLVAQLAERKLTLYSTLNYEGSMRWLPAHPLDA
                     SVERYFNSDQRRDKGFGPALGPDACAYLSQCLTAHGYNVILAESPWQLGQSQRALSVQ
                     LIAGIYRALKEQPLNEAAQLHPTALAHWRQFRLEHLFNSACYVGHMDLLALPPE"
     gene            complement(981..2603)
                     /locus_tag="PKZ52_09325"
     CDS             complement(981..2603)
                     /locus_tag="PKZ52_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60015.1"
                     /translation="MLSSPFKAAFGTVFIRSALVPILALSAIFLALALPSNFTDLQDG
                     YWLYVLPGELLLIMLALSLPRSYRAIYYLIAMLLCASLFLKIADLGMWRVYARPFNVL
                     LDGPLLLKGVDLLTGVLGKAWASIIILLASLALIGLLFLLGLGLLAVQRRLQQQAIIW
                     LRLTGAGIIFWLVCAVLGWPGARWPTANFIWLRAQELYASWQDLTLFSAQLTQAPLPV
                     SSSTALLEKLKGHDVLVVFVESYGRTLLSHPTSAEHFGTFLDNQYAQLQQQGYSARSH
                     LLTSSTIGGISWLAHATTLSGLWINNQARYDLLLKSQYPSLNQLFRQAGWRSVGIMPA
                     ITRDWPEGTYYGYDQFYGAQDLLYKGVAFNWITMPDQYTLYAFEQIERNKKNRANIFA
                     EIVLVSSHAPWTPTPTVLPWSMLNEPHAFDKMVQEGASPEWVWQDTQRIRQFYERSIE
                     YVLGALSAYIQAFGDDHLVVVILGDHQPAPLITEHDGNKDVPIHIISKDQQVMEALDQ
                     WHWREGLRPALNAAWPMDSLRNRWLEAFSTQH"
     gene            complement(2607..3758)
                     /locus_tag="PKZ52_09330"
     CDS             complement(2607..3758)
                     /locus_tag="PKZ52_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNG60016.1"
                     /translation="MWFDCNAVIFSLRNYSQTTHPAYLALAALNCLLLSLLCASLAAS
                     LLRPTDSLAVYYGAGALLGVAANVFFSRYPKTNSRPLRRNLLRATLFYAALYLIIYYL
                     IGPLLWFELRFYPRDARHSICCETPTDYGATEYEAVLLNTADGGKIAGWYIPARERLG
                     KVVVLIHGSGYDRRGTDFYARILLKQGYGILMYDQRNHGESSGPLNTFNRMDKMQGDL
                     VAVITYLENEKHLAPSSIGIVGISLGGFAALNLPAATINRIGPLWLDGIRSDNFGVYA
                     SGNVLSDTARQLFDYQTRLLAGLLTSEPIAAPAKSFRTLLPAVKQANIQLVASGLDNQ
                     ERKTNEGLIPYLSPSMSLWIIPNAWHIGGRFDAKDEYEQRMTAFFNNHL"
     gene            complement(4090..5358)
                     /locus_tag="PKZ52_09335"
     CDS             complement(4090..5358)
                     /locus_tag="PKZ52_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG60017.1"
                     /translation="MNDLYIHPLDARALQSKRRILSLRQLPYLALGFLFCAAAMRLFI
                     NTQNANALAEYTAATLERRVLITQAKPGELQRTLALPTTLRGNNESIIYARSTGYEAA
                     WHKGIGDAVQKGDLLAVLDAPEQDQELAQARAQREQIAARADFAKQTLARWETLQVRS
                     ALSEQDLAEKRSTLRQVNADLAAADANVKRLEQLQKFRHITAPFNGVLTRCSVEVGDL
                     IANTGKELFAITQIDPLRLTVWVPQAYAANLQLGAQVNVNIAEYPGAKLTATIERIAG
                     GIDPATRARQVDLVLKNPRASLLPGAYAEVSIQLKSDVKTLIAPAASLIVSDQTQRVA
                     IVDEQQKIRFQTVKLGRDLGRDVEILEGISPSDNLVVSPPDQLVAGEQVKTLDWKPAR
                     PEQAPPGNKPTGAEKSADSEKNNLTEKKSK"
     gene            complement(5365..>6238)
                     /locus_tag="PKZ52_09340"
     CDS             complement(5365..>6238)
                     /locus_tag="PKZ52_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG60018.1"
                     /translation="NEANGNTYTIGARSLETDLTNIDQLMQIPIGKSSDGRQQLLGNV
                     VSYKRVAQSSIVSRYNNSNMVNIHASIQGRDLGSVSREIEKVLEKTRANLPRGVDVQL
                     RGQIQTLKTTYTGLGIGLVVAIALVYFLVVVNFQSWLDPLIIISALPAALAGIAWLLF
                     LSGTPLSVPALTGVIMTIGVATANSILLVSFARSQLHKGISPINAALTAATTRLRPVL
                     MTAFAMIIGMLPMALGLGEGGEQNAPLGRAVIGGLLFATVSTLLFVPVVFATLHRIQA
                     ARAPRHSAFPLFAE"
BASE COUNT         1575 a         1536 c         1587 g         1540 t
ORIGIN      
        1 gctttagcgg cagcattgcg ccatgtttta ttagatgcaa gtgctgcaca tgttatggca
       61 gcggcggctc gtagagccgc gagtagtttg ccccgttggg agcatacagc agaaataatt
      121 ggtggttggc ttcagcaatt gagtgagagg cggtgatttt atgcagaaaa ctttatccac
      181 aattgaaccg tttacccccc agtggttgga tgtgcgcgag ccggcggata aagcggctcg
      241 ggacaagggt ttacaggcca tgtttatcga ctggttaaac acacacaaca cgccgccgaa
      301 aattttggat ttgggtgcgg gcacaggtgc gaatctgcgc gcgctgtata aaccgaacca
      361 gtcttggcgc ttagttgatt gggataacac cctattaaac gaagccctgc gtcgccaccc
      421 gcagtactat gtcaccaccc ataaagccaa tttaaatgca ttggagagtt taccctggga
      481 tggagttaca gcggttactg cctcggcatt atttgattta acctctgcgc agtttattga
      541 tcagttagtg gcgcaattag cggaacgaaa attaacgctc tacagcacgc tcaattacga
      601 gggctccatg cgctggttgc cagcgcaccc cttggatgca agcgtcgagc gctattttaa
      661 tagcgatcag cgccgcgata agggttttgg cccggcccta ggcccagatg cttgcgctta
      721 tttatctcag tgtttaaccg cgcacggcta taacgtaatt ttagcggaaa gcccatggca
      781 gctcgggcag tcccagcggg cattgagtgt gcaattaatt gccggtattt accgcgctct
      841 taaagagcag ccactgaatg aggctgcgca attacatcca acggcattag cacactggcg
      901 gcaatttcgc ttagagcatt tatttaatag cgcctgttac gttggccata tggatttatt
      961 ggccttgccg ccagagtaaa ttaatgctgt gtggaaaatg cttctagcca tcgattgcgc
     1021 agagaatcca tcggccaggc agcattaagc gcagggcgca agccttcgcg ccagtgccat
     1081 tgatccagtg cctccatcac ctgctgatct ttactgataa tatgaatcgg gacgtcttta
     1141 ttgccgtcat gttctgtaat cagcggggcg ggttggtggt cacctaaaat aaccaccact
     1201 aaatggtcat caccaaaagc ttgtatataa gcgctaagtg cgcctaatac atattcaatc
     1261 gagcgctcat aaaattgccg aatacgctgt gtatcctgcc acacccattc tgggcttgcg
     1321 ccttcttgca ccattttatc aaaggcgtga ggctcattaa gcatgctcca agggagcaca
     1381 gttggcgttg gcgtccaggg tgcgtgggag gaaaccagca caatttctgc aaaaatattc
     1441 gcgcggtttt ttttattgcg ctctatttgt tcaaatgcat acagggtgta ctgatccggc
     1501 atggtgatcc agttaaacgc cactccttta tacaataaat cttgcgctcc gtagaattga
     1561 tcgtagccat aataagtccc ttccggccag tcgcgggtaa tggcaggcat aatgcctacg
     1621 ctgcgccaac cggcctggcg gaataattga ttgagtgatg gatactggct tttaaggagt
     1681 aaatcgtagc gggcttgatt attaatccac aggcccgaaa gcgtagtggc atgggccaac
     1741 caactgatac cgccaatagt tgatgaggtt aataaatggc tgcgggcaga atatccctgc
     1801 tgctgcaatt gtgcatattg gttatctaaa aaagtgccga aatgttctgc tgaagtagga
     1861 tggctgagta atgtgcgccc ataggattct acaaacacca ccaatacatc gtggcctttg
     1921 agtttttcca acaaagcagt tgatgaggat acaggtagtg gcgcttgggt taattgggcg
     1981 gaaaacagcg ttaaatcctg ccagctggcg tacaactcct gagcgcgcag ccagataaaa
     2041 ttcgcggtgg gccagcgcgc cccaggccag cctaaaacgg cacacaccag ccagaaaata
     2101 atccccgccc ctgtgagcct aagccaaata atggcctgtt gttgcaatct tctttgcacg
     2161 gccagcaaac ctaaaccgag caaaaaaagc aaacctatta gtgcaagact cgctaacaaa
     2221 ataataatac tcgcccaggc tttgcctagc acgccagtga gcaaatcaac gcctttcagt
     2281 aacagcgggc cgtcgagtaa aacattaaat ggccgcgcat aaacccgcca catgccgagg
     2341 tcggcaattt taaggaaaag gcttgcgcac agcagcatgg cgatcaggta ataaatagcg
     2401 cgataactgc ggggtaggct aagcgccaac atgatcagca ataattcgcc aggcaatacg
     2461 tacagccaat aaccatcttg taaatcagta aaattgctgg gcagtgccag ggctaaaaat
     2521 attgcgctta gcgcaagtat cggcaccagc gctgagcgga taaataccgt gccgaatgcg
     2581 gctttaaagg gtgacgataa cacgggctat aggtggttat taaaaaatgc tgtcatgcgc
     2641 tgttcgtatt catccttggc atcgaagcgg ccgccaatat gccaggcatt gggaataatc
     2701 cacagggaca tggaggggct taaataggga attaaccctt cattggtttt gcgctcttgg
     2761 ttgtctagcc cactggctac caattgaata ttggcctgtt taactgcagg caacagggtg
     2821 cgaaaactct tggcaggtgc tgcaatgggc tcgctggtaa gcagcccggc caataaacgg
     2881 gtttggtaat caaataattg ccgagctgta tcgctcagca cattacccga tgcatacacg
     2941 ccaaaattat ccgagcgaat gccgtccaac cacaagggcc cgatacggtt aatagttgcc
     3001 gccggtaaat ttaacgccgc aaaaccgcct aaagaaatac ccacaatacc gattgagctg
     3061 ggcgctaggt gtttttcgtt ttccaagtag gtaatgactg cgactaaatc cccttgcatt
     3121 ttatccatac gattaaatgt gttgagcggg ccgctggatt caccgtggtt gcgttggtcg
     3181 tacattaaaa tgccataacc ctgttttagc agtatacgcg cgtaaaaatc agtgccgcgc
     3241 cggtcatagc cggaaccgtg gattaacacc accactttgc ctaatcgctc acgggcgggg
     3301 atataccagc cggcaatttt accgccatcc gcggtgttta ataacaccgc ttcgtattcg
     3361 gttgcgccgt aatccgtagg agtctcgcag caaattgaat ggcgcgcatc gcgcgggtaa
     3421 aagcgcaact caaaccacag cagtgggcct attaggtaat aaataattaa atacagcgct
     3481 gcataaaaaa gtgtggcgcg cagtagattg cggcgcaggg gcctagaatt ggtctttgga
     3541 taacggctaa aaaaaacatt ggcggctaca cctaaaaggg cgcctgcgcc gtaatagaca
     3601 gccaaagaat cggtaggccg cagtaggctc gctgccagtg atgcacagag caggctcagt
     3661 agtaaacagt tgagtgcggc gagtgccagg taggcaggat gtgtggtttg actgtaattg
     3721 cgcagtgaaa aaatgacggc attacagtca aaccacacat cctgcctacc tggcactcgc
     3781 cgcacaggta ggcaggatgt gtggtttgac tgtaattgcg cagtgaaaaa atgacggcat
     3841 ttgacatgga atatacaaaa atttatcgga tgaacgtgta caagcggggg cggcctgcgc
     3901 tgactatata ataaggtgtt ttattcttct ggcggcacaa taagtcgttc cgatctattt
     3961 tgccttcaca gcgccgccac ttgcgagaat gcgtggattt ttttagcgca gttcgcgacg
     4021 gcgtgcgccg caggtttggc agccttgcca ccattattaa ccccgcgcta aatgccgccg
     4081 ggttaataat cacttggatt ttttctcggt caaattgttt ttttcgctgt ccgcgctttt
     4141 ttcggcaccg gtaggtttat tccctggggg tgcctgttct ggccgggcgg gtttccagtc
     4201 gagggttttt acctgctcac ccgccactaa ctgatccggc gggcttacca ctaaattatc
     4261 gctagggcta atgccctcga gtatttccac atcgcggccc aggtcgcggc ccaattttac
     4321 ggtttgaaag cgaattttct gttgttcatc cacaattgcc acccgctgag tttgatcgct
     4381 cacaatcaaa cttgccgccg gggctataag ggtttttaca tcacttttta attgaatact
     4441 cacctcggca taggcgccgg gtaaaaggct ggcgcgggga ttttttaaca caagatcaac
     4501 ttgccgcgcc cgtgtggcag ggtcaattcc ccctgcgatg cgctcaatcg tcgcggttaa
     4561 tttcgctccg gggtattcgg caatattgac gttaacctgc gctcccaatt gcagattcgc
     4621 agcataggct tggggcaccc aaaccgttaa gcgcagcgga tcaatttgtg tgatggcaaa
     4681 taactctttg ccggtattgg caattaaatc gcctacttca acactgcagc gtgttaaaac
     4741 gccgttgaac ggcgccgtaa tatggcgaaa tttctgcagc tgttctaagc gttttacatt
     4801 ggcatcggcg gcagctaaat ctgcattcac ctggcgcagg gtgctgcgtt tttccgctag
     4861 atcctgctcg gataaagcgc tgcgtacctg tagcgtttcc cagcgcgcca gtgtttgctt
     4921 ggcaaaatcg gcgcgcgcgg cgatctgctc gcgttgggct cgcgcttggg caagttcttg
     4981 gtcttgttcc ggtgcatcca gcacagctaa taaatcacct ttttgtactg catcgccaat
     5041 gcctttatgc caggccgcct cataacctgt actgcgcgcg taaataatcg attcattatt
     5101 gccgcgcagg gtggtaggca aagccagggt gcgctgtaac tcaccgggtt ttgcttgggt
     5161 aattaatact ctgcgctcca gtgtggcggc ggtgtattcc gctagggcat tagcattttg
     5221 ggtgttgata aataggcgca tggcagcggc acagaataaa aatcccaggg ctaaataggg
     5281 cagctgccgc agggacaata tccgccgttt actttgcagt gcacgagcat ccagtgggtg
     5341 aatataaaga tcattcatta acaatcactc agcaaaaaga ggaaatgcac tgtggcgcgg
     5401 tgcacgggcc gcttggatgc gatgcagtgt ggcaaatacc accggcacaa ataacaaggt
     5461 cgagacagtg gcaaataaaa gcccgcctat aacggctcgg cctaaaggtg cattttgttc
     5521 tccaccttca cccaagccca gcgccatggg caacataccg ataatcatgg caaacgccgt
     5581 cattaatacc ggccgcaaac gcgtggtggc agcggttaag gcggcattta taggggaaat
     5641 gcctttgtgt aattggctgc gggcaaatga aaccaataaa atactgttcg ctgttgctac
     5701 ccctatggtc ataattacgc cggtgagcgc aggtacactt aaaggggtgc cagataaaaa
     5761 taataaccaa gcgatacccg ctaatgctgc gggcagggca ctaataataa tcagcgggtc
     5821 aagccaggat tgaaaattca ccaccactaa aaaatacacc agggcaatag ccaccacgag
     5881 ccctataccc aaacctgtgt aagtggtttt aagggtttgt atttgcccgc gcagttgcac
     5941 atccacaccg cggggcagat tagcgcgggt tttctctaat actttctcga tctctcggct
     6001 caccgagcct aaatcccgcc cttgaatact cgcgtgtata ttcaccatgt tgctattgtt
     6061 gtagcggctg acaatagatg attgcgccac ccgtttatag ctcactacat tacccaataa
     6121 ttgttgtctg ccgtcgctgc ttttacctat agggatctgc attaattgat cgatattggt
     6181 aaggtccgtc tctaaactgc gggcaccaat tgtataggta ttgccattgg cttcattt
//
LOCUS       DAOQGS010000213         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6239_length_6234_cov_5.213627, whole genome shotgun sequence.
ACCESSION   DAOQGS010000213 DAOQGS010000000
VERSION     DAOQGS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6239_length_6234_cov_5.213627"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PKZ52_09345"
     CDS             complement(<1..1314)
                     /locus_tag="PKZ52_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60019.1"
                     /translation="MKHLLLLPCLFGLIGCGQGSESSSRPNTSAAAEVVAPSGNTSSS
                     SSSSSSGIILANSITNRPTSSSSSSSSSSSSSSTSSSSSSSSSSSSSSNSSGLPRAAS
                     SSSSSSSSSSSSSSSSSSSSSSSSSGRSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSGLTLASHVHLDVIQAKCVACHKANGTAKTSDLIFTRGDTSVEGANLTILNTFVL
                     NESALRVLNKVKGLDNHGGSDVLAPSDPEYIWLATYLGEKSGQNLTAYAQVKTTLTLE
                     GPDATYRRASLLLTGKIPSSQKLSGLHQANDETLRKALADLLEDPGFKEFIKRGANDQ
                     LLVRSLVVTKNYLDDLIFYYPIFNQKFQSIDPATQLEKSDYVYGDAVLNEVAEAPLEL
                     IAHVVQNNLPYTEVITAPYTMVGEKTAEIYKTGLTPAAGTFVPAVN"
     gene            1775..2029
                     /locus_tag="PKZ52_09350"
     CDS             1775..2029
                     /locus_tag="PKZ52_09350"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HNG60020.1"
                     /translation="MSQTILPVLIPHERVGEETLQRLLEDFATRDGTDYGATELSLTQ
                     RVQLIHQQLKRGEAKLVYFPQDESVQLVTEREAKMMGLED"
     gene            2447..2683
                     /gene="tusA"
                     /locus_tag="PKZ52_09355"
     CDS             2447..2683
                     /gene="tusA"
                     /locus_tag="PKZ52_09355"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HNG60021.1"
                     /translation="MSDPVAHYFLDAEGLYCPEPVMLLHGLVRKAAVGEIVHVLATDP
                     STERDIPKFCRFLGHELVKTRADVQRFEYWICKK"
     gene            complement(2889..3590)
                     /locus_tag="PKZ52_09360"
     CDS             complement(2889..3590)
                     /locus_tag="PKZ52_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60022.1"
                     /translation="MKTSNSTHNTNVIDLFTRKPYVPLEQKRIIRLALELDGLEMLYS
                     NDTNSDRLFSLKILAWGLRANGEVVGLVPWLNDLIPCSEIHDPVHGHWEGYYDPGIDE
                     IFTEPPMHKVIELETAAEYFNYQCETPDEIVQELPDTLGTHAVFSDSKNKTLILQEII
                     SWRLHSDGVILGMLIDPEKVQNTPVLPGDDCLFPAQAKDNFRYFFQHHIANKIKSEDP
                     EALAAMCLLVEDSSR"
     gene            3809..4396
                     /locus_tag="PKZ52_09365"
     CDS             3809..4396
                     /locus_tag="PKZ52_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HNG60023.1"
                     /translation="MQSGEYSYAAAQSSVPEISGEVLIRLFNELFVPQVRTCLCGGAE
                     EPIYLPASAVRQFNEIHFTRDYCASALHEIAHWCVAGKERRKQIDFGYWYAPDGRSQE
                     QQAQFEQVEIKPQALEWIFSVAAGMRFRVSADNLAQGIGASVAFKRAVAQQAQYYCSV
                     GLPVRAAQWAQTLADFFCVSDPLDSRHYKYEFLGE"
     gene            4400..5506
                     /locus_tag="PKZ52_09370"
     CDS             4400..5506
                     /locus_tag="PKZ52_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNG60024.1"
                     /translation="MLHLVADENIPFLNDFFGHFGRITALPGRAMQASDLASADILLV
                     RSVTKVNAQLLTDSPVKFVGTCTIGVDHLDAEYLARRGIITASAPGCNAGGVVQYVLS
                     ALARIRPQWPQAKIGIVGCGNVGGRLYRVLNAAGVNVCAFDPFKQHADIRWAAWEELL
                     QCDIICMHTPLTRTGVAPTFHLFNETVLRRLKPGTLLLNAGRGEVIDNHALLKCLQDK
                     QDLTVVLDVWEGEPEILAPLMDCVALGTPHIAGYSYEGRVNGSLMIHRALGEFLNVNE
                     ADLTRHETQVMSRAMGEKECLNFTDMNSSLLSIYDVDRDDRQLRALVKQEGTSRFGFD
                     QLRKNYPQRRELSHYVVSPQSPQVHTLRALGFAV"
     gene            5563..>6234
                     /locus_tag="PKZ52_09375"
     CDS             5563..>6234
                     /locus_tag="PKZ52_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNG60025.1"
                     /translation="MAHKGSDNLHLPDYLLANGQNLHSFKRTADFCLALGEMAKNITW
                     WAPKGLMGGDVLQSLGIAFQSTAAEVTASSSALDTQAVVAEYCSKKVDLIVFAGGDGT
                     AADVAQVIARLNPAQLALGIPAGVKIQSSVFAINPTAAGQLVAALVRGELLHVESAQV
                     RDLDEVALQQGQVKSRYLGTMLVPYDGRFVQSIKQGGLVAEELIISDIAAYLKELIPS
                     NATVVF"
BASE COUNT         1510 a         1337 c         1672 g         1715 t
ORIGIN      
        1 gttaacggct ggcacaaagg tgccggcggc aggtgttaag ccggttttgt aaatttccgc
       61 agttttttca ccgaccatgg tgtagggggc ggtgatcacc tctgtgtagg gcaaattgtt
      121 ttgcaccaca tgggcaatta actccagagg tgcttctgca acctcattta ataccgcatc
      181 accataaaca taatcggatt tttccaactg cgtggcgggg tcgatacttt ggaatttctg
      241 attaaaaata gggtaataaa aaattaaatc atcaagataa tttttggtaa ccacaaggct
      301 gcgcaccaac aactgatcgt tggcgccacg cttaataaat tctttaaagc cagggtcttc
      361 tagcaggtcg gccagtgctt tacggagggt ctcgtcgttg gcttgatgca agccactgag
      421 tttctggctg ctgggaatct tgccggtcaa taataacgat gcgcgacggt aggtagcgtc
      481 gggcccttcg agggtcaggg tggttttaac ctgcgcatag gccgtcaaat tttgcccgct
      541 tttctcgcct aaataagtgg ctagccaaat atattcaggg tctgatggcg ctaaaacgtc
      601 actgccaccg tggttatcga ggcctttcac cttattaagc acccgcagtg ccgattcatt
      661 gagcacaaag gtatttagga ttgtaagatt ggccccttcg acactggtat caccacgggt
      721 aaaaataagg tcagaggttt ttgctgtgcc gttggcttta tggcaggcaa cacatttggc
      781 ttgaataaca tccagatgta catggctggc aagcgtgagc cccgaggagg agctgcttga
      841 gctagatgag ctactggatg aagacgagct gctagagctt gagctgctgc tggatgacga
      901 actgcttgaa ctgctacttg atgacgaacg gccagaactg gatgagctgc tagaactgga
      961 tgagctgcta gaactggagg agctgctaga gctcgatgag ctgctagagc tggctgcccg
     1021 cggcaaaccc gaggaattac ttgagctgga actactagag cttgaggaac tgcttgaact
     1081 cgttgagcta ctggagctgg acgaactgct tgagcttgac gagctagtgg ggcggttggt
     1141 aattgaattc gctaaaatta tcccgctact gctggaggac gagctagaag atgtattgcc
     1201 agagggggcg acaacttcag cggcagctga tgtgttgggg cggctggagg actcgctacc
     1261 ttggccgcaa ccaataagcc cgaacaaaca gggtagcagt aacaaatgct tcatatacag
     1321 cctcagcaga aaaaccaacc tactgaacca acttgccgtt ggctcatggt aagcctatga
     1381 aggcaaaagc agcttcgcca aacacggatg atggttttgt tacccatgtc acgaatcctg
     1441 attaaataga cgaagcgtta gaagagttgg ctatggcagt gaaattctga caccatattg
     1501 tcataagttg cgagtttggt tacaaccgtt atttgccccc tgggtttaac aacctcggct
     1561 aataggctga ttttggcgcg aattatcaca acgaccgcgg gcggcaggcg tgacatgatc
     1621 ggggtaaatt cttgcggcgc ctgcttaggt cttaattcgt gactaatttt gcatgcgcac
     1681 atgtttcccc tgtcttaagg tgcgggcatt tttgcctcag taactgctta gagcgcactc
     1741 taggtttaat tagcggttct ttaattgtga tgtgatgagc cagacaatcc taccagtact
     1801 aatcccccat gaacgtgtag gcgaagagac actgcagcgt ttactggaag atttcgctac
     1861 ccgcgacggc actgattatg gcgcgacgga attatcactt acccagcggg tgcagttgat
     1921 tcatcaacag ttaaaacgcg gtgaagctaa gttagtgtat ttcccccagg atgaatcagt
     1981 gcagctggta actgagcggg aggcaaaaat gatgggattg gaggactagt tttgcaaatg
     2041 ttggttttgc agcctactaa ctaaaaaatc ttaagtgaac tttggcggct taatcttggc
     2101 gaaaagtccg catttattcc atgtaaagcc tgcttatttc gccaaatata atgccgctag
     2161 agctagggaa cctctgaaaa attaccttac tcgctctggc gtcgttgaat ttgccaaaaa
     2221 aatgctcatt tactgcgtgt aaactcggca attgctcctg cattgctcta cctcctgcat
     2281 ccatgcagtc gtccgctttt tttggcaaat tcgcctagcc agagcttcgc cgccgacatt
     2341 tttcagaggt tccctagcaa tgtagtattc aacaaactgc tagatggtct tgccgacctg
     2401 tttgagcgcg ttgctagcta ggtttgatac gttttctgga gtgcttatga gtgaccccgt
     2461 tgcacattat tttctggatg ctgagggcct ttattgtcct gagcctgtga tgctgctgca
     2521 tggattggtg cgcaaggccg cggtaggtga gattgtccat gtattggcaa ctgacccgtc
     2581 caccgagcgt gatattccca aattctgccg ctttctcggc cacgaacttg taaaaacccg
     2641 tgcagatgta cagcgttttg agtattggat ttgcaagaag taagtgggct tgtgttttat
     2701 aaacgccggt ggctttttta atacctaatt ttaatgccta actgcgcctt cagtgcgtgc
     2761 gctatgtcgt agttgttgta ttcgcaacaa accacttgcg tgcataaagc cgcacacaag
     2821 cggtcggctg aagagtttac tgcccaagta ggggctacca gtgggcaaca agtacatggt
     2881 gcgtgcgatt aacgactgga gtcttccacc agcaaacaca tggcagcaag tgcttctgga
     2941 tcttccgatt tgattttgtt ggcaatgtgg tgttggaaaa aatagcgaaa attatctttt
     3001 gcttgtgccg gaaaaagaca atcgtcaccg ggtaatacgg gtgtgttttg cactttttca
     3061 gggtcgatca acatgcctaa aatcacccca tcactgtgca ggcgccaact aataatctcc
     3121 tgcagaatga gcgttttatt tttgctatcg gaaaatacgg catgtgtgcc tagtgtgtct
     3181 ggcagctctt gcactatttc gtcaggcgtt tcgcactgat aattaaaata ttcagcggca
     3241 gtttctaatt caatcacctt gtgcatgggc ggctcggtaa agatttcatc gatacctgga
     3301 tcgtaataac cttcccagtg gccgtgtact ggatcgtgta tctccgagca ggggattaaa
     3361 tcgttcaacc agggcactaa accgaccacc tcaccgttgg cccttaaacc ccaggcgagt
     3421 atttttaggc taaacaagcg gtcggaattg gtatcgttgg agtagagcat ttcgaggccg
     3481 tcaagctcaa gggcgaggcg gatgatgcgt ttttgctcga gtggaacata gggtttgcgg
     3541 gtgaatagat cgatcacgtt ggtattgtga gtcgagtttg aggtcttcat aatactacct
     3601 ccgcaaaaaa tgcaggcgaa gtgccgccta cggcagaatg gtaaggtacc atcagtactt
     3661 acagtatatg cacaaagcgt ttaattcaac acccacagca ataaaaaaat tttaagccat
     3721 caagacgagt ttaattcaac ccattccccc taacgtaagt ccaagttcgc aggcaacctc
     3781 ttagcctgcc cgatatgtgg agagaaaatt gcaatcaggt gagtactctt atgcggcggc
     3841 ccaaagttcg gtgcctgaaa ttagcggcga ggttttaatc cggctgttca atgaattgtt
     3901 tgtgccgcag gtgcgcacct gcttgtgtgg cggtgctgaa gaacctatct atctgcctgc
     3961 tagtgcggtg cggcaattta atgagattca ttttacccgc gactattgtg catcggcatt
     4021 gcacgaaatt gcccattggt gtgtggcggg caaagagcgg cgtaagcaga ttgattttgg
     4081 ttattggtac gcgcctgatg ggcgttcaca ggagcaacag gctcaattcg agcaagtgga
     4141 aatcaaaccc caggcactgg agtggatttt ttcagtggct gccggtatgc gctttagagt
     4201 aagtgctgat aatttagcgc agggcattgg ggcaagtgtt gcgtttaagc gggcagttgc
     4261 gcaacaggcg caatattatt gttctgtggg cttgcctgtg cgggccgctc aatgggcgca
     4321 aaccctagct gatttttttt gtgtttcgga tccgctggat agcaggcact ataagtacga
     4381 atttttaggc gaataaaata tgttgcattt agtggcggat gagaatattc cgtttttaaa
     4441 cgattttttt ggtcattttg gccgtatcac ggcattgcct gggcgcgcta tgcaggcgag
     4501 tgacttggct tcggctgata ttttgttggt gcgttcggtc actaaggtga atgctcaatt
     4561 actaacggac agcccggtaa aatttgttgg cacctgcacg attggcgtgg atcatctgga
     4621 tgcggagtat ttggcacggc gtggaattat cacagccagt gcgcccggtt gtaatgccgg
     4681 tggcgtggtg cagtatgtgt tgagcgcttt ggcacgtatc cgaccccagt ggccccaggc
     4741 gaaaataggt atagtggggt gcggtaatgt gggcggccgg ttgtatcgtg ttttaaacgc
     4801 agcgggtgtc aatgtgtgtg cgtttgatcc ctttaaacag catgcggata ttcgctgggc
     4861 tgcatgggag gaattattac agtgcgatat tatttgtatg cacacacctt taacccgcac
     4921 aggggtggca ccaacttttc atttgtttaa tgagacagtt ttgcgacgct taaaacctgg
     4981 caccctatta ttaaatgcgg gccgtggtga ggtcattgat aatcacgcgt tgttaaaatg
     5041 tttgcaggac aaacaggatt tgaccgtagt gctggatgtt tgggagggtg agccggaaat
     5101 cctagctccc ttaatggact gtgtggcgct tggtacaccg catattgctg gatatagtta
     5161 tgaaggccga gtgaatggtt ccttgatgat tcatcgcgct ttaggtgaat ttttaaatgt
     5221 aaatgaggct gatttaactc ggcacgaaac ccaggtgatg tctcgtgcta tgggtgaaaa
     5281 agaatgcttg aatttcaccg atatgaattc atctttgttg tcgatttatg atgtggatcg
     5341 cgatgatcgc cagctgcggg cactggtaaa gcaagagggt acatcgcggt ttggctttga
     5401 ccaattgcgt aaaaattatc cccagcggcg ggaattatcc cactatgtgg tttctccgca
     5461 atcgcctcag gtacacacct tgcgtgcttt aggttttgca gtttaatgcg gccattaacc
     5521 gtaggtttag taataaatcc cctggctggc gtaggtggcc ctttggcgca caaaggcagc
     5581 gataatttac atttgccgga ttatctactc gctaatggac agaatctgca ctcctttaaa
     5641 cggacagctg atttttgtct ggcgcttggt gagatggcga agaatataac ctggtgggca
     5701 cccaaaggtc ttatgggggg cgatgtttta cagtcgctgg gaattgcatt tcaatccacc
     5761 gcagctgaag tcaccgcatc ttcttccgct ttggacacgc aggccgtggt ggcagagtat
     5821 tgcagcaaaa aagtagattt aattgtgttt gccggtggtg atggaactgc cgctgatgtg
     5881 gcacaggtaa ttgcgcgttt aaatcctgcg cagctagccc ttggtattcc tgcgggggta
     5941 aaaatacaat cgagtgtttt tgccattaat ccaactgccg ctggccaatt ggtggcggct
     6001 ttggtgcgcg gggaattatt acatgttgag tcggcacagg tgcgggattt ggatgaagtg
     6061 gcgttgcaac aaggccaggt caaaagccgt tatttaggta ctatgctggt gccctatgat
     6121 gggcgttttg tgcaaagcat aaaacaaggc ggcttggtgg ctgaagaatt aattattagt
     6181 gatatagccg cctatttaaa agaattaata ccgagtaacg cgacagtagt gttt
//
LOCUS       DAOQGS010000214         6215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6268_length_6215_cov_4.584416, whole genome shotgun sequence.
ACCESSION   DAOQGS010000214 DAOQGS010000000
VERSION     DAOQGS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6268_length_6215_cov_4.584416"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKZ52_09380"
     CDS             complement(<1..717)
                     /locus_tag="PKZ52_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNG60026.1"
                     /translation="MLFKFSAFTKRYKNHLGATIKLITLLVSIPQLTGCHRQSASEFP
                     AINFATCTPPKGHNPIPESGCAFISVPENPANPNGATIDVHFLRIKAVNARPQADPLI
                     LIQGGPGGSSQIMAYSLYRYFASVRQNRDLLFVDLRGTGESNPLNCEGLETITSRLSD
                     KDALQTYRALMQTCAQKYAERAPFYSTELAAADLEILRRRLGYQQFNLWGASYGTRVA
                     LKYAVNYPDFARSLVLDGLAP"
     gene            1499..2317
                     /locus_tag="PKZ52_09385"
     CDS             1499..2317
                     /locus_tag="PKZ52_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG60027.1"
                     /translation="MPGSSPDFITIRNLSFFRGDRAIFDGLNMSIARGKITAVMGPSG
                     TGKTTLLRLIGGQLAPDAGDIILEGQNIPALSRNDLFKVRRKIGMLFQSGALFTDLSL
                     YENVAFPLRVHTQLPENIIRDIVLMKLEAVGLRGAYRQMPNELSGGMARRAALARAIA
                     LDPELVMYDEPFVGQDPISMGMLVKLIRVLRETLGITSIIVSHDVNETASIADYIYVV
                     AGGKVIGEGTPESVLNTQDPQVKQFIHGLADGPVPFHYPAADYRADLGLIQVPR"
     gene            2314..3102
                     /gene="mlaE"
                     /locus_tag="PKZ52_09390"
     CDS             2314..3102
                     /gene="mlaE"
                     /locus_tag="PKZ52_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNG60028.1"
                     /translation="MSVLTAVQRLGAATLTNLEVLGRASILWFRAVFARPDWRNAPAL
                     LIRQIYFVGVYSLIIILVSGLFIGMVLALQGYTVLVRFGADQALGQLVSLSLLRELGP
                     VVAALLFAGRAGSALTAEIGLMKTTEQLASMAMIGVDPIGRIVRPRLWAGMISMPILA
                     QLFNSVAIFGAVLVGVNWLHVDAGSFWSNMQQSVDFTNDVLNGVIKSLVFGAVISWIA
                     VFQGFDATPTSEGISAATTRTVVYSSLAVLGLDFLLTAVMFGVG"
     gene            3103..3570
                     /gene="mlaD"
                     /locus_tag="PKZ52_09395"
     CDS             3103..3570
                     /gene="mlaD"
                     /locus_tag="PKZ52_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNG60029.1"
                     /translation="MRSAKLEISVGAFVLAGFCALAFLALQVSGLNLSTQQQKTYTLF
                     ARFSNASGLSERAKVSVAGVVVGRVAHIALDPLDNRAKVEMVINRDVNFLTQDSIASI
                     QTAGILGEKYIAIANGGAPEILADGGEITDTQSALVLEELIGKLLTSLASKKD"
     gene            3573..4292
                     /locus_tag="PKZ52_09400"
     CDS             3573..4292
                     /locus_tag="PKZ52_09400"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNG60030.1"
                     /translation="MQKFICVLLLSVSCQLSPWVWADTATAPTPGTAATVNVKGDPYQ
                     LVQQTSQAVLDYVRQVSADYEKDPSRYNAQVLAIMDPVVDFDYFARSVMGTYASEARY
                     NSLKTDAEKQAFKANVQRFSDMFKQGLVDTYAKYLFKFTGDKIETLPPRKGDDTNSGQ
                     VAVMQNIKTGNNKPFVVQYSMRKNKAGEWKLQNVIVEGINLGQTYRTQFAQAADKYHG
                     DLTAVINNWQVTPQTAAEGSP"
     gene            4289..4585
                     /locus_tag="PKZ52_09405"
     CDS             4289..4585
                     /locus_tag="PKZ52_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNG60031.1"
                     /translation="MSSAQLQCVDNTLSLSGLIDHTSVVSLLQQGQQFVSSADPQELI
                     VDWAQVAYANSAALAMVLQWTRQAQAAGKSLRSQNVPEFLVQLARVSQLEFLFT"
     gene            4994..5620
                     /locus_tag="PKZ52_09410"
     CDS             4994..5620
                     /locus_tag="PKZ52_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNG60032.1"
                     /translation="MLPTEQGKTYVLIRDKRAPCDVLYFGGNAEAVDASQRDLERAFS
                     QCNILSMIYRGYGLSEGTPSQAHLYADALALYRLRQAKTREFIIVGRSLGTSLATYLA
                     SQVPVKQLVLVTPFDSMANLVSDKFPWLPTGLLLKNPFPSAEYARHVTAPVHILLAEE
                     DEIIPTSHTLALQKAFIRTQPQLTRLGKVGHNTVDEHPAYIQVLGRRL"
     gene            complement(5626..>6215)
                     /locus_tag="PKZ52_09415"
     CDS             complement(5626..>6215)
                     /locus_tag="PKZ52_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNG60033.1"
                     /translation="VLQASVAVADMFMDGGLVVYTQSRLPQSNEKFFAQPKDLTNALP
                     LVVLINEGSASASEIVAGALQDSKRALILGTRSFGKGSVQTVVPLTDEKAMKLTTALY
                     FTPNGRSIQAQGIEPDIRVERASITRAENENLSVSEAELSGHLANGTGGKEIKAQERQ
                     KERDKAANNLQTKDNQLFEALNLLKAINLLSAPKS"
BASE COUNT         1479 a         1402 c         1634 g         1700 t
ORIGIN      
        1 gggcgcaagg ccatcgagca ccaaactgcg cgcaaagtcc gggtaattaa cggcgtattt
       61 taaagcgacg cgggtgccat aggaggcgcc ccacagatta aattgttgat agcctaaacg
      121 cctgcgcaga atttcaagat cggcggcagc caattcggtg gaataaaaag gcgcccgctc
      181 ggcatatttt tgtgcgcagg tctgcatcag tgcgcggtag gtttgcaacg cgtccttatc
      241 gctaagcctt gaggtaatgg tttcaaggcc ttcgcaattg agcgggtttg actcaccggt
      301 gccgcgcaaa tcgacaaata ataaatcgcg gttttgccgc acggaggcaa aataacgata
      361 cagagaatag gccataattt gtgacgagcc gcctggccca ccctgaatta atatcaaagg
      421 atcggcctgg gggcgggcat taacggcttt tatacgtaaa aaatgcacgt cgatagtagc
      481 gccatttgga ttggcggggt tctccggcac gctgataaat gcacagccac tttcggggat
      541 tggattatgg cccttgggcg gcgtacaggt ggcaaaatta atggcgggaa attcgctggc
      601 ggattggcga tggcagcctg ttaactgggg gatgctgact agaagcgtaa taagttttat
      661 ggtggcgccg agatggtttt tgtaccgctt ggtaaaagcg gaaaacttaa aaagcaaatg
      721 ccatttgaac gtgcaaacaa tatttgtcga taaatagcgc agtctgtctt gggacttaat
      781 caggctggaa ttaatggctg tcagttgtaa aagatttatt gcccaacaaa aagctggctc
      841 gggtgctgga ggtttttggt attttgttgt ggtagtacca gggaaaacca ttgtggaact
      901 catgcagcag atttgaggaa gataaaatac gctaatactc gatttacacg ctaggctaat
      961 gtcagccagg cggcctaaat agttttgtgc taatgcgctt caatgccaaa tgccattcaa
     1021 tgcaaaatgt tgcaggccta gggaacctct gaaaaatgtg ggcggcgaag cgctggctag
     1081 gcgaatttgc caaaaaaagc gcagtttaca cacagtaaat gagcattttt ttggcaaatt
     1141 caacgacgcc agagcgagta aggtaatttt tcagaggctc cctaggttta aaccgggcat
     1201 ccatccaata gactgactat caaattggtt aatagtaggc ttggcgctta agtatagctt
     1261 ggctccgttg ctgtggcctg tgtttagcgt atgatttcct cgggccacaa cctcctggct
     1321 ttgctgcaat aaggagtaat cccggccgat tgcgcggttg gcgtgggtgg atatgccagc
     1381 ttgatgagaa attccggcta aatcagccta aattccagca cttgttggtt gctcctctgg
     1441 ttgaaccatc ttgccagaga gtatactaag ccccctcttc attgcgctta gcgtccttat
     1501 gcccggctcc tcacccgatt tcatcaccat tcgcaattta tctttttttc gcggcgaccg
     1561 ggcaatattt gatggtttga atatgtccat agcccggggc aagattaccg cagttatggg
     1621 cccctcaggc accggcaaaa ccacactttt acggctaatt ggcggccagc ttgccccgga
     1681 tgctggcgat atcatccttg aagggcagaa tatcccggcc ctaagccgca atgatttatt
     1741 caaagtgcgg cgcaaaatcg gcatgttatt tcaaagcggc gccttattta ccgatttatc
     1801 tctgtacgaa aatgtcgcct ttcccctgcg ggtgcacacc caattaccgg aaaatattat
     1861 tcgcgatatt gtcctgatga aactcgaagc ggtgggtttg cgcggtgctt accggcaaat
     1921 gcccaatgaa ttatccggcg gtatggcgcg cagggccgct ctggctcgcg ccattgcact
     1981 cgacccagaa ttggtcatgt acgatgagcc ttttgtaggt caagacccta tttccatggg
     2041 catgttggtt aaattaattc gcgttctgcg ggagacattg gggattacca gcattattgt
     2101 ttcccacgac gtcaatgaaa ccgctagcat cgccgattat atttacgtgg ttgcaggcgg
     2161 taaggtcatc ggtgagggta caccggagtc tgtactgaat acgcaggatc cgcaggtaaa
     2221 acaatttatt cacggccttg ccgatgggcc tgtgccattt cattatccgg cagcggatta
     2281 ccgtgcggat ttaggtttaa tacaggtgcc aaggtgagtg ttttaactgc ggttcagcgc
     2341 ctgggggcgg caaccctaac caacttagag gtgctggggc gagcctctat cctgtggttt
     2401 cgggcggttt ttgctcggcc agattggcgc aacgccccgg ccttattaat tcgccaaatt
     2461 tattttgtcg gcgtttattc gttaattatt attttggtgt ctggattatt tatcggcatg
     2521 gtgctagccc tgcaaggtta taccgtgtta gtgcgttttg gtgctgatca agccctcggg
     2581 caattggtgt cgctgtccct gctgcgggaa ttagggccgg tggtggccgc tctattgttt
     2641 gctggtcgcg caggctctgc cctaacagcg gaaattggcc tgatgaaaac cacagagcaa
     2701 ctcgcctcca tggcgatgat tggcgtggat cccatcggcc gtattgtcag gccgcgcctg
     2761 tgggcgggaa tgatttctat gccgatattg gcgcagctat ttaatagtgt ggctattttt
     2821 ggcgctgtat tagtgggcgt aaactggttg catgtggatg ccggttcgtt ttggagcaac
     2881 atgcagcaga gcgttgattt taccaatgat gtattaaacg gtgttatcaa gtctttggta
     2941 tttggtgcag ttattagttg gattgcggtt tttcagggtt ttgacgccac gccaacttcc
     3001 gaaggcatca gtgctgccac cactcgcaca gtggtctatt cctccttagc tgttttaggc
     3061 ttggattttt tattaacagc agtcatgttc ggagttggtt aaatgcgttc tgcaaaactg
     3121 gaaatttcag tcggcgcttt tgtgttagcg ggtttttgtg cgttggcatt tttagcgctg
     3181 caggtgagtg gcttaaatct ttccactcag caacaaaaaa cctacacact atttgcccgg
     3241 tttagcaatg catccggttt atccgagcgt gctaaggtgt cggtggcggg agttgtggta
     3301 gggcgcgttg ctcatatagc tttagatccg cttgataatc gggcaaaagt tgaaatggtt
     3361 attaatcgcg atgtgaattt tttaacccaa gacagtatcg cctcgattca aacggcgggt
     3421 attttagggg aaaaatatat agctattgcc aatggtggtg caccggaaat attggccgat
     3481 ggcggtgaaa ttaccgacac ccagtcggcc ctagtgcttg aagaacttat cggtaaatta
     3541 ttaaccagtc ttgccagcaa gaaggactaa ttatgcaaaa gtttatatgt gtgttgttgt
     3601 tatcggtcag ttgccagtta tccccatggg tatgggctga cacagcaaca gccccgacac
     3661 ctggcacagc ggcaacggtc aatgtgaaag gcgatccgta tcagttggta caacaaacgt
     3721 ctcaggcagt attagattat gtgcgccagg tgagcgccga ttatgaaaaa gaccctagcc
     3781 gctataatgc acaggtgctc gcgattatgg atccggtggt ggattttgac tattttgccc
     3841 gctcggtaat gggtacttat gccagcgaag ccagatacaa cagcttaaaa acggatgcgg
     3901 aaaaacaagc ttttaaagcc aatgtgcagc gctttagcga tatgtttaaa cagggtttgg
     3961 tggataccta cgctaaatat ttgtttaaat ttactggcga taaaattgaa accctgccgc
     4021 cgcgtaaagg tgatgacacc aactcgggtc aggtggcggt gatgcagaat attaaaaccg
     4081 gcaataacaa accgtttgtt gtgcagtaca gtatgcgcaa aaacaaagcg ggcgagtgga
     4141 agttacaaaa tgtgattgtg gaaggcatta atcttggcca gacctaccgc acgcagtttg
     4201 ctcaggcggc tgataaatac cacggtgatt taactgccgt tattaataat tggcaggtga
     4261 cgccccaaac ggcagctgag ggttcccctt gagtagtgcc caattgcaat gcgtcgataa
     4321 taccctgagt ttaagcgggt tgattgatca tacgagtgtt gtcagtcttt tgcagcaggg
     4381 gcagcaattt gtgtccagcg ctgatcctca agaattgatt gtggattggg cgcaggtggc
     4441 ctacgccaat agcgccgccc tcgccatggt gttgcagtgg acgcgacagg cccaggctgc
     4501 aggtaaatcc cttcgcagcc aaaatgtacc tgaatttttg gtgcaattgg cgcgggtaag
     4561 tcagttggag tttttgttca cctaaaatga gttatacagg ctgcaacaag gatacgcagt
     4621 tgaacctaaa aaccgcagtt gaactctact acggccgcct aatgcttgaa tctgcacgct
     4681 tttttgtcaa aatttgactc accgtagtgg tgactactgc tttcgccaaa ttttgacaaa
     4741 aaagcgccca tattcagcgc cttaggcacc ctcgctacga gttcaactgc ggtttttagg
     4801 ttgaatataa ctacttacag cagatggcgc gtcataacct gttgataata aattctccat
     4861 gcgtcttatt gtgcgattaa tagtgctgag tgcactttct tatttcggta tttgtgccgg
     4921 gctctatttt gcgcaagatg cgcttatttt tccttcccat ttggtttctt tgtcagctag
     4981 tggccaacgt cttgtgctgc ctactgagca gggtaaaacc tatgtattga ttcgcgataa
     5041 acgggcccct tgtgatgtgc tgtatttcgg tggcaacgca gaggcggtag atgcaagcca
     5101 aagggattta gagcgggctt ttagtcagtg caatattctt tcaatgattt atcgcggtta
     5161 cggtttaagt gaaggcacgc caagccaagc ccatttatat gccgatgcgc tggccttata
     5221 tcgcttacgg caagcaaaaa cccgtgaatt tattattgtc ggccgcagtt tgggcaccag
     5281 ccttgccact tatctggcct cccaggtgcc ggtaaagcaa ttagttctag tgacaccctt
     5341 tgatagcatg gcgaatttag tcagcgataa attcccctgg ctgccgacgg gattgctact
     5401 aaaaaatccc tttccgtccg ctgaatatgc gcgccatgtg acagccccag tgcacattct
     5461 gctcgctgaa gaagatgaaa ttattcccac ttcgcacacg cttgccttgc agaaagcttt
     5521 tattcgcact cagccacaat taacccgctt gggtaaggtg ggacacaata cggtggatga
     5581 acacccggcc tatatccagg tgctcggccg ccggttgtaa ataggtcagc ttttgggggc
     5641 ggataaaaga ttaatagctt ttagaagatt aagcgcttca aacagctgat tatctttagt
     5701 ttgtaaatta ttggcggctt tatcgcgctc tttttgtcgc tcttgggctt ttatttcttt
     5761 gccgccggtg ccgttggcta agtggccgct taattcggcc tcgctcacac tgaggttttc
     5821 gttttcggcg cgggtaatac tggcgcgctc cacgcgaata tccggctcaa taccctgggc
     5881 ctgaatggag cggccgttgg gggtaaaata cagggcggta gtgagtttca tggctttttc
     5941 atcggtgagc ggtaccacgg tttgcaccga gcctttgccg aaactgcggg tgcctaaaat
     6001 taatgcccgt ttagaatctt gcagggcgcc tgcaactatt tcggatgctg aggccgagcc
     6061 ttcattaatt aaaaccacca agggtagggc gttggttagg tctttgggtt gggcaaaaaa
     6121 tttctcatta ctttgcggca agcggctttg ggtatacacc acaagcccgc catccataaa
     6181 catatcggcc accgccactg aggcttgcaa aaccc
//
LOCUS       DAOQGS010000215         6177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6326_length_6177_cov_4.562235, whole genome shotgun sequence.
ACCESSION   DAOQGS010000215 DAOQGS010000000
VERSION     DAOQGS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6326_length_6177_cov_4.562235"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKZ52_09420"
     CDS             <1..724
                     /locus_tag="PKZ52_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60034.1"
                     /translation="SGLKISPDNPQLDTQPAPNGLSSPRIDSLSVVPNQSGTLEVPAV
                     EITWWDTQADQLRVASLPAQPLLISAASQPSANLPSLPNPAIPQLEPATAAKPVAPAW
                     LYALSGGLALSNLIFIGLWLSARAQIRGAKAPAERKVTTKELWSQVTQALQQQNPKSA
                     REALLLWFQREAAQPFTNLTEIAQWANDPTLAEALKTLDGAIYSSNSRAWQGQLLLDA
                     LLSWREKTKSQQAADNDLAPLY"
     gene            866..3277
                     /locus_tag="PKZ52_09425"
     CDS             866..3277
                     /locus_tag="PKZ52_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60035.1"
                     /translation="MFLTSRTSFLLSVLISLSLTGCGGNKNKNSAPAVSSSSSSSSSS
                     SSSSSSSSSSSSSGVAGPDTQGPSVNVIFPGVNNFTTADSVTLRGKASDQTGIKTLTI
                     NDLAVTTSNQWADWQLKLSGLKDGENVLTLKAEDTLGQTTEQVLRITKQLPMFAPELS
                     ALDASGNVLFIYDSYVKAIFAVDLSTGSRRVLTMSKSGEANGFSGPTAMIYDPGKDRL
                     LVTDLSVISVTGFSDYYVGRIISINAKTGERSQFAVGDSLPDISKPSLSLRAPVSMTI
                     DPASRSLYVLDPKSGFISNNQGQLNFDYAILKYPLDSDAPSFTLVSENQNNKNDVFVG
                     SRRIRFDRDNNRLITDFQFGEQTNTLGNNESIYGLMAVDTSTGARTIISGKNVHADNT
                     YAFKITQNADFFVDAGFVYYIDRAPDTDANSERILKINLTSGERAEWFGNKAADNKFN
                     LRTLITLNYDSTGKQLYAIDYSLDTVYKINTLNNFQRTPVASNGVARENTAINYLSSN
                     ALLWDKRHQRLLLNDRSDGNVLAYNLATGTPSIFCSFGSIDQTSIVYQPVDSALDETN
                     QRLISLVNAQYISDNQIFNSSRVDSCDLATGATSVLSKGTSLAATSFTVPTTALALDA
                     HNKAYLFETALRQDSSNYYEENIITQVDLTTGTRSEYAALKSKESPKGALAVNAASGQ
                     VVYSQLSNASINTIDPTTKVISAVTNNLKDNSTLLLIPKKLFWLDNELITLDSARKTL
                     LSIQPDGKRSLRYSINATGPNPINQVSGMAIDTEQQRLFITDQATGVLALQDLVTGES
                     IYLDK"
     gene            complement(3483..4457)
                     /gene="cysB"
                     /locus_tag="PKZ52_09430"
     CDS             complement(3483..4457)
                     /gene="cysB"
                     /locus_tag="PKZ52_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNG60036.1"
                     /translation="MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDEL
                     GVEIFSRSGKHLTRITPAGQAILRTAGEILRKVESIKQVAQEFSNERKGSLSLATTHT
                     QARYALPKVISTFIERYPEVSLHMHEGTPIQISEMAAEGSVDFAIATEAMELFSDLIM
                     LPCYTWNRCLLVPKNHPLCQVSKITLEDLAKHPLVTYVFGFTGRSRLDEAFMEHGLAP
                     KVVFTASDADVIKTYVRLGLGVGIVAKMAYEPETDTDLVAIDASHLFRPSTTRIGFRR
                     GTFIRGFMFEFIELFAPHLTKDVVNEAFNRHSKIEIDELFVNVKLPHY"
     gene            5292..5831
                     /gene="folE"
                     /locus_tag="PKZ52_09435"
     CDS             5292..5831
                     /gene="folE"
                     /locus_tag="PKZ52_09435"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNG60037.1"
                     /translation="MQDLFAQIITQVGEDITRPGLKDTPLRAAKAFATLTKGYSQSLE
                     EVVNGALFPSDASEMIIVKDIELFSMCEHHLLPFIGKAHVGYIPAGKVLGLSKVARIV
                     DMFARRLQIQEQLTHEIATTIQQVTGAVGVGVIIEAKHLCMMMRGVEKQNSVMKTSAM
                     LGNFRSNSATRTEFLSLLR"
     gene            5936..>6177
                     /locus_tag="PKZ52_09440"
     CDS             5936..>6177
                     /locus_tag="PKZ52_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNG60038.1"
                     /translation="MAAFDLRYPRPLSGGSTRSMGKPVALQVFARVLVGALALTASIS
                     QAGQYYKWVDDKGVTHFSEKPVTGMDAKKMGTSSKTA"
BASE COUNT         1616 a         1654 c         1456 g         1451 t
ORIGIN      
        1 cagcgggtta aaaatctccc cggataaccc ccagctggac actcaacccg cccccaatgg
       61 cctctcctct ccccgtatcg acagcctctc ggtggtaccc aatcaaagcg gcaccttaga
      121 agtgcccgcc gttgaaatca cctggtggga cacccaggcc gaccaactgc gggtggcgag
      181 cctgcccgcc cagccactgc tgatttcggc tgcaagccaa cccagcgcca acctgccgag
      241 cctgcccaac ccggcaattc ctcagctgga gccagccaca gcggcaaaac ccgttgcgcc
      301 cgcctggctc tatgccctca gcggcggact agcgctatcc aacctaatat ttatcggcct
      361 gtggctatcg gcccgggcgc agataagggg ggccaaagca cctgccgagc gcaaagtcac
      421 caccaaagag ctctggtccc aagtcaccca ggcgctgcaa caacaaaacc ctaaatccgc
      481 ccgagaggcc ttgctgctgt ggttccaacg ggaagcggcc cagcccttca ccaaccttac
      541 cgaaatcgcc caatgggcca acgacccgac actcgcggag gcacttaaga ctctggatgg
      601 ggcaatctac agctccaaca gccgcgcctg gcagggccag ctattactgg atgccctgct
      661 cagctggcgg gagaaaacca aatcgcaaca ggctgcggat aatgacctag cccccctgta
      721 ctaaccggca gccccctcgc accccgcccg cgcgcaacac agcgggcatg gggaattggc
      781 gggcggttca caatagattg gccatcacag ggccaacctt ctacaatgcg ccgctttttt
      841 atttcactta cctgatgatc attctatgtt tttaacctcc cgcacttctt tcctactatc
      901 agtactgatc agcctctcgc tcacaggctg cggcgggaac aaaaataaaa actcagcccc
      961 ggcggtatcg agcagctcaa gttccagctc ctcatccagt tccagctcgt caagttcttc
     1021 cagcagttca tccagcggtg tggcggggcc tgatactcag gggccgagtg ttaatgttat
     1081 tttcccgggg gtaaacaact tcaccacggc tgatagcgta actctgcggg gtaaagcgag
     1141 tgatcaaaca gggattaaga ctctgactat caacgatcta gccgtcacca caagcaatca
     1201 atgggcagat tggcaactta agctgagcgg ccttaaggac ggtgaaaatg ttttaaccct
     1261 caaggccgag gacacgctgg ggcagaccac tgaacaagtc ctgcgtatca ccaaacaact
     1321 gcctatgttc gcgcccgagc tgtcagctct ggacgcctca ggcaatgtgt tgtttattta
     1381 cgacagctac gtaaaagcta tttttgccgt cgatctttcc accggtagcc gcagggtgtt
     1441 aaccatgtcc aaaagcggtg aagccaatgg attttccggc cctaccgcca tgatttatga
     1501 cccaggcaaa gatcggttgc tggtgaccga cttaagtgtc ataagcgtga cagggttcag
     1561 tgattattac gtaggacgta ttatcagcat caatgcaaaa accggcgagc gctctcaatt
     1621 tgcagtggga gatagtttac ccgacatctc caaaccctca ctttcactgc gtgctccggt
     1681 atcgatgacg attgatccgg catcgcgaag cctttacgtg ctggatccca aatcaggttt
     1741 tatcagcaac aatcaaggcc aattgaattt tgattacgcc atattaaagt acccactgga
     1801 ttcggatgct ccgagtttta ctttggtcag cgaaaaccag aataataaaa acgacgtgtt
     1861 cgtcggcagc cgccgcattc gttttgaccg cgacaataat cggctaatta ccgatttcca
     1921 atttggcgaa caaacaaata cccttggcaa taacgaatcc atttatggcc ttatggctgt
     1981 tgacacatct acaggtgcgc gcactattat ttctggcaaa aatgtccacg ccgacaatac
     2041 ctatgcgttc aaaatcacac aaaacgcgga cttttttgtt gatgcaggct tcgtctacta
     2101 tattgaccgc gccccggata cggatgccaa ctcagagcgc atattaaaaa tcaacctcac
     2161 cagtggcgag cgcgccgagt ggtttggcaa caaagccgcc gacaataaat ttaatctgcg
     2221 cacattgatc acactcaatt acgattccac tggcaaacaa ctatatgcga ttgattattc
     2281 actcgatacc gtctacaaaa tcaatacgct gaataatttc cagcgcacgc cagttgcaag
     2341 taacggtgtt gccagagaaa acaccgccat aaattacctt tcgtccaatg cactgctgtg
     2401 ggacaaacgc catcagcgtt tactattaaa tgaccgcagt gacggcaatg tactcgccta
     2461 caacttagcg accggcacac cctcaatttt ttgcagcttc ggcagcatcg accaaacctc
     2521 tattgtatat caaccggtgg atagcgcttt ggacgaaact aaccagcggt taatttcttt
     2581 ggtgaatgca cagtatattt ccgacaacca aatatttaac agctcgcggg ttgatagctg
     2641 cgatttagca actggcgcaa ccagcgtttt atctaaggga acctcgcttg ccgcaaccag
     2701 ttttaccgtc ccaacaacgg ccttagcact ggacgcccat aacaaagcct atttatttga
     2761 gactgcgtta aggcaggatt ctagtaatta ttatgaagaa aatattatta cccaagttga
     2821 cctaaccacg ggcacgcgct cggaatacgc agcactaaaa tcgaaggaat cgcccaaagg
     2881 cgcattggca gttaacgccg cgagcggaca agttgtgtat tctcaactat ctaacgccag
     2941 catcaacact attgacccaa ccacaaaagt tatttccgcg gttacgaata atttaaaaga
     3001 caattcaaca ctgcttctga tccctaaaaa acttttctgg cttgataatg aattaattac
     3061 tctggattcc gctcgcaaga ctttgttgtc cattcaacct gacggcaagc gcagtcttcg
     3121 ctacagtatt aatgccacag gcccaaatcc catcaatcaa gttagcggca tggcgataga
     3181 cactgagcag cagcggctat ttattaccga ccaagccacg ggtgtattag ctctgcagga
     3241 tttagttacc ggtgagagta tttacctgga taaatagctg atagtagagc atggaggcaa
     3301 ggcccaagga gtgggcacat taaacaatac tgggtatctt gaagttatat ggtacacgtg
     3361 tcctaagcac cagaacccat tactaaaaaa atcaccagca aatactaaaa acccctgccg
     3421 ctacgccaat aactcagcgc agcggcaggg gttttgcgtg ttctgtacgt taccaacttc
     3481 gattaataat gcggcaactt cacattcaca aacaattcgt caatttcaat tttggaatga
     3541 cgattaaacg cctcgttaac cacatcctta gttaaatgcg gggcgaataa ttcaataaac
     3601 tcgaacataa agccgcgaat aaacgtcccc cgtctaaaac cgatccgcgt tgtgctgggg
     3661 cggaacaagt ggctggcgtc tatggcgact aaatccgtat cggtttcagg ttcataggcc
     3721 attttagcca ctatgcccac gcccaagcct aagcgcacat aggttttaat cacgtccgca
     3781 tctgaggcgg taaacactac ttttggcgcc aggccgtgct ccataaaagc ttcatccaag
     3841 cgagagcggc cggtaaagcc gaatacgtag gtgactaatg gatgtttggc taggtcttct
     3901 aaagtgattt tgctcacctg gcacaagggg tgatttttcg gcaccaataa acagcggttc
     3961 caggtgtagc agggcagcat gatgaggtcg ctaaataatt ccatcgcctc ggtagctata
     4021 gcaaaatcca cactgccttc ggcggccatt tcagaaatct gaatcggcgt gccctcgtgc
     4081 atatgcaggc tgacctcggg gtagcgctca ataaaagtgg aaattacttt aggcagcgcg
     4141 taacgggctt gggtgtgggt agtggccagc gacaagctgc ccttgcgctc gttgctgaac
     4201 tcttgcgcta cctgcttaat gctctccacc ttgcgcagta tttcccctgc ggtgcgcagg
     4261 atcgcctgcc cggctggggt aatacgcgtt aagtgtttac cgctgcggga aaatatttct
     4321 acgcccaatt catcttccaa caaccgaatt tgtttactaa tacccggttg tgatgtgtag
     4381 agactttgcg ctgtagccga tacattaagg tcgtgatgcg ccacttccca gatataacgc
     4441 aactgctgca acttcataat tcctcccaat ggtagcttgt gtaagccttc cgcagattag
     4501 ctaaggtggt atttttaggg tgtataaact ataaaaacct acgtccttag aagcgtaggt
     4561 aaatttcgtt cattttgcca gcccccagag agtttattgt tattaactcg acaactatgt
     4621 gctgcgcgct aatcacataa caggcgcaaa aaagtacctg gcaaatttcg cagcaacagt
     4681 taattcgcag caagaggtat cagcacaacg attggcacct aaaccctggg cacaggcacg
     4741 gctcttttag ccaagctaac agggtgcgtc acggcaaggc ggccaataca tcattcgggg
     4801 cgctcaactt gctggcggct tcacttaagc tgcacgggcg ctgttttaaa tctaaaaact
     4861 gcagttgaac ttgtaacgag ggtgcctaag gcgcggagca tgtgcgcttt tgggtaaaac
     4921 acggcaccac ctagggtaag cccctttttt accgaaaaac ctgcagatcc aagcattagg
     4981 cagtcctagt ggagttcaac cgtggttttt aggttaaacc taaaaaccac ggttgaactc
     5041 gtagcgaggg tgcctaaggc gctgaatatg ggcgcttttt tgtcaaaatt tggcgaaagc
     5101 agtagtcacc actacggtga gtcaaatttt gacaaaaaag cgtgcagatt caagcattag
     5161 gcggccgtag tagagttcaa ctgcggtttt taggttaaac cccttggaac ctatcgcaag
     5221 acttttcaag cggcggcgtt tggcttaggg tagccgccct aattttcgga tcactgtgtt
     5281 ggatgtgtgt tatgcaagat ttatttgccc aaatcattac tcaagtaggt gaggacataa
     5341 cccggccagg ccttaaagac acacccttgc gggcggccaa agcctttgcc accctcacca
     5401 aaggctacag ccagtccctt gaagaagtgg tcaatggcgc cttatttccc tccgatgcca
     5461 gcgaaatgat cattgtgaaa gacatagagc tcttctccat gtgcgagcat catttattgc
     5521 catttatcgg caaagcccac gtggggtata tcccggcagg taaggtattg ggcctgtcca
     5581 aagtggcgcg gattgtggat atgtttgccc gccgcttaca gattcaagaa cagctcaccc
     5641 atgaaattgc caccaccatt caacaggtca ccggcgcggt gggcgtaggc gtcattattg
     5701 aagctaagca tctgtgtatg atgatgcggg gggtcgaaaa acagaattcg gtgatgaaaa
     5761 cctccgccat gctgggcaat ttccgcagca acagcgccac tcgcacggaa tttttatccc
     5821 tgttgcgcta gcctgattcc tactgcacta gcccatagcc tgtgcgctac aacaaggtgt
     5881 tttagttaaa gggcggctac acttaggcaa acggttatat aacacgaggc gatttatggc
     5941 ggcttttgac ctgcgttacc cgcgccccct ctcagggggg agtacccgct cgatgggaaa
     6001 acctgtggcg ctgcaggtgt ttgcccgcgt tctagtgggc gcactggcct taaccgccag
     6061 tattagccag gctgggcagt actacaaatg ggtggacgac aagggcgtta ctcatttttc
     6121 ggagaaaccg gtcaccggaa tggatgctaa aaaaatgggc acatcctcta agaccgc
//
LOCUS       DAOQGS010000216         6161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6351_length_6161_cov_4.180314, whole genome shotgun sequence.
ACCESSION   DAOQGS010000216 DAOQGS010000000
VERSION     DAOQGS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6351_length_6161_cov_4.180314"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6161
                     /locus_tag="PKZ52_09445"
     CDS             <1..>6161
                     /locus_tag="PKZ52_09445"
                     /inference="COORDINATES: protein motif:HMM:NF017412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HNG60039.1"
                     /translation="SATSATQLTLNVSGALDNQQGRVLSDSLSLTAGSLDNSNGYIDA
                     QSTQGKTLIALTGDAQNGQGTLLSRQNELDITTANWQNNQGQIGHLGSGLFRLQAQQM
                     DNSAGKVQTFGSADWQLTGWNNAQGKVSIRGDGAVGSSIVLSGDWLNDQGRLLVGDDG
                     FTFTARQLINTSQDDTQGIWHSGNGHWTLKADTFTNRAGTVGGAGGLQFELQHLENAG
                     QIDGQTLHLTAQSVANAGVINLSDRAATSLDSQIQGDWLNQGGKISLAGGGVFAAAHW
                     DNTQGQLLAAGAISVTASGGDADKVSFLNAQGWLQADMGLALTTANTLDNRQGTLLSG
                     GTIQLTSQAGINNTSGTIDLTPSNIPGALTPTVSISAAGEFDNTQGVVQSFSHGSQTL
                     STSQINNTQGLIYSQAQSFTAMVAQVDNTGGRWQHTGQTLEIKGLKEWRNAANTSSAA
                     LMQTDGTLVIQGFERILNSGQVGEAPSTIRARSAQLQGKRLENLSGAQLALVAPDSSS
                     ALDITEAETAGIPKPLASLAPVDSSLQLDQLVNQGGIFTQSNLDLGKAHLLNTGGQLS
                     SLNDLTLSAADLGTLGGTLWAEGTLTLTSEGDFTFANQAKLDAGRGIVWDVKGAFTNQ
                     GSLASGSDLTLKAASFNNSQTSDVSAVGALALELTQSLTNQGRIRGASDTQVQANSIA
                     NNGLIASQKALNLSATATLTNTDTLFATGDLTALAPELFNTSSAATTASIWAGGKLTL
                     AGGKTTTGGRLSVGKQLINTSGSIQTYRSGDIELAFNQLTNRTNELRLKSEPTSAKVW
                     FTTGSGKPDADIGNGEFDLTVNELVNLLARSGNQLPYTKHNLYVYQPAVPITQPRINS
                     GGALRIQTQNEFTATQGSCSGDACTPGAIQNIGGAIQAARDITIDLGYRQQGASAQPS
                     GSLLNGNIYLPGEATHERKVFTSITPDPGVSGTETAKFSIIGTFSYGSGISVSPETVE
                     KAASLFTGSILAGGSLTTYSQSITNEGAAAYQAAWTGHTPGDAPTMSFSQAAPFSRIP
                     LSATSVAPVGSTERTALSPVNPNAQGLVGDGASEVTTPERPQTTPVAQLEARIYNPLQ
                     NLVLPNNGYLYRVDIKGSSPYVVQAADLIKPPTDKPATPPTSSDYFFTQLGYDPEQIG
                     RRLGDSFYETELVRQAVMNTRGVRFVTDPRTGLAIDSDEAQYRYLLDNALEAQKDLQL
                     AVGTALTPEQVRALTQDIVWLERHELAEQEVWVPVYYATGVDPNQMANGAVMAGQSVR
                     VVSEELLNRGDIRGLSLVDVSSQKSQNFGAIGSLDRLIVNSQSVDNKGLMLGKTTQVS
                     GFDIDNSGQMIARETLDVTAENELNNRGELAGTDVNAKSTGADLINQGRINASNNADL
                     DAARDILLKPGQGASINTGGNLSATAGRDFNLEGQAISTQGDATISAARDAAITPVTE
                     RSLISGGHTSTEVITNTATRITTGGSLSIDAGRDVGITATKIDTGKDVTLRGGRDVNL
                     TSAADSTHTEGQGKNWHSIETDVKQVTTDINAGGKIDATAGRDITMVAVQGRAEGDIH
                     VNAERDTNLLGAVDSQYDYDYEKKKKSFGRSKTTESESLKETAVATELSAGGNLLLNT
                     KLGSNGEILKETGQRINTEGAKLSAGDPKALAQAPAQAPDTPSNKAADKPQGTIAAYA
                     ADGITLAGQSTKSLDYSETRRSGTLGLSKKAKGSNEGEDHSSATTLTSTGDTQLLSDH
                     NLTLEGVVANVNGDMTLSAVDDLRVAPGETHTQTQTWDNKSGAFSGGSLYRKTETNSE
                     DGETGLTDSALLVKGNLKVDAGSTEILGTRIQTGSRDENGNFTATTDSGNLTLGAAQA
                     SKFHKDSTLDVRLKGTDTLKALKEPHKIVENDDGRAKIKLGSATYLNADNSSTTLSQQ
                     GTEVSTFGDITLDATSIGGKTNLAGSTLNSTGIIDLHGTQSLALSSVDATQSEQHKTQ
                     EGKGEASFVVQHQAVEVAKAAKNVLDAKEQLSDAKRQLKHYENNLDTQQSQLNQLRAD
                     LAAGTPGVTADDVQLLSELVRDLEDDRAWYQTGLALA"
BASE COUNT         1587 a         1914 c         1623 g         1037 t
ORIGIN      
        1 cgtccgctac cagcgcaact cagttaaccc tgaatgtatc gggtgcactg gataaccagc
       61 agggtcgcgt gcttagcgat agcctaagcc tcaccgctgg cagcctggac aacagcaacg
      121 gctacatcga cgcccagagc acccagggca aaacgctgat agcgcttact ggcgatgcgc
      181 agaatgggca gggcaccctg ttaagccgac aaaatgaact cgatatcacg accgccaact
      241 ggcagaacaa ccagggccag atcggacatt tgggcagcgg gttattcagg cttcaggccc
      301 agcagatgga taacagcgcc ggtaaggtgc agaccttcgg cagtgccgat tggcagctaa
      361 ccggctggaa caatgcccag ggcaaagtca gtattcgcgg cgacggcgct gtgggttcgt
      421 ccattgtact tagcggcgat tggctgaatg atcagggccg cttactggtg ggcgacgacg
      481 gtttcacctt taccgcgcgg cagctgatca acactagcca ggacgatacc cagggtattt
      541 ggcatagcgg caatggccat tggacgctta aagctgacac ctttaccaac cgtgcaggca
      601 cagtgggtgg ggcgggtggc ctgcagtttg aactgcaaca ccttgaaaat gccgggcaaa
      661 tagacggcca aacacttcat ctcacggccc aatctgtcgc caatgcaggc gtgattaacc
      721 tttctgaccg tgcggcgacc agcttggatt cacagataca gggtgactgg ttaaaccaag
      781 gcggcaaaat cagccttgct ggaggcgggg tatttgcagc ggcacattgg gataacaccc
      841 aaggccaact tttggccgct ggcgccatca gcgtgaccgc aagcggcggc gatgcggata
      901 aagtctcgtt tctcaatgct caaggctggc tacaagccga catgggatta gcgctcacca
      961 cggctaacac actggataac cgccagggca cgttgctctc cggcggcaca atccaactca
     1021 ccagtcaggc cggaattaat aacaccagcg gcaccataga ccttacgccc tcgaacatac
     1081 caggcgccct tactcccacc gtgagcattt cggctgctgg ggagtttgat aacacccaag
     1141 gggttgtgca gagctttagc cacggcagcc agaccctcag caccagtcag atcaacaaca
     1201 cgcaagggct catttacagc caagcccagt cattcacagc gatggttgcc caggtggata
     1261 acaccggcgg ccgctggcag cacaccgggc aaacgctgga aattaaaggg ctcaaggagt
     1321 ggcgcaacgc cgccaacacc agctccgctg cattgatgca aacggacggc accctggtaa
     1381 ttcagggctt cgagcgaata ctcaactccg gccaagtggg cgaagccccc agtaccatcc
     1441 gcgcccgcag tgcacagttg cagggaaagc gccttgagaa tttgagcggt gcgcaattgg
     1501 ccttagtcgc gccagattca tcaagcgcac tagatataac cgaagccgaa acagcgggca
     1561 tcccaaagcc actcgcgagc ctcgcgcccg ttgactccag ccttcagctc gaccaactgg
     1621 tgaaccaagg cggcatcttt actcaatcta acctagatct tggcaaggcc cacctgctca
     1681 acacgggcgg ccagctgtcc agcttgaatg atctcacgct gagcgccgcc gacctcggta
     1741 ccctaggcgg caccttgtgg gctgagggca ccctaacgct taccagcgag ggtgatttca
     1801 ccttcgccaa tcaagcgaag ctggatgccg ggcgagggat tgtgtgggat gtaaagggcg
     1861 ccttcaccaa tcaaggctcc ttagccagtg gctcggattt gaccctaaaa gccgcatctt
     1921 ttaacaacag ccaaacctcg gatgtgtcgg cagtgggtgc gctcgcgctt gagttgactc
     1981 agtcccttac caaccagggt cgtatccgag gcgctagcga cacccaggta caggccaata
     2041 gcattgctaa caacgggctt atcgccagcc aaaaagccct taacctaagc gccacggcca
     2101 cgctcactaa cacggatacc ttatttgcca cgggcgatct caccgcactg gcgccggagc
     2161 tgtttaacac cagctcagcc gctacgacgg cgagtatttg ggccgggggc aaactgaccc
     2221 tggcgggtgg caaaaccacc acaggcggga ggctaagcgt tggcaaacaa ctgatcaata
     2281 cgtccggcag cattcaaacc taccgcagcg gtgatattga gctggcattt aaccaactca
     2341 ccaatcgcac caacgagctg aggctcaaga gtgaacccac ctcagccaaa gtctggttca
     2401 ccaccggttc gggcaagccc gatgctgaca ttggcaacgg tgaattcgat ctcactgtga
     2461 atgaactggt aaatctactg gcgcgcagtg gcaatcaatt gccctacacc aagcacaacc
     2521 tatacgtcta ccaaccggca gtccccatca cacaaccgcg tattaactct ggcggggcgc
     2581 tgcgcatcca gacgcaaaat gagttcacgg ccactcaggg ctcgtgcagt ggtgacgcgt
     2641 gtacgccagg cgccatccaa aatatcggcg gtgctattca agccgctcgc gatataacca
     2701 ttgatttggg ctatcgccaa cagggcgcca gtgctcaacc cagcggcagc ctgctcaatg
     2761 gcaatatata tctacccggt gaagccaccc atgagcggaa agttttcacc agtatcacac
     2821 ccgaccccgg agtttcaggt accgagacgg ccaagttttc catcatcggc accttctcgt
     2881 atggcagcgg tatttccgtc tcccctgaaa ccgttgagaa ggcggccagc ctgtttaccg
     2941 gctcgatttt ggccggaggc agcctcacca cctacagtca atcgatcacg aatgaaggtg
     3001 ccgccgccta tcaagcggcc tggacaggcc acaccccagg tgacgcaccg acgatgagct
     3061 tcagtcaggc cgcgcccttt agccgcattc ccttgagtgc cactagtgtt gccccagtcg
     3121 gttcgactga gcgcacggca ctctcccctg tgaatcccaa cgcccagggg ctagttggcg
     3181 atggagccag cgaggtcacc actcctgagc gcccacagac cactcctgtg gcacagctag
     3241 aagcgcgtat ttacaacccc ttacaaaacc ttgtcctacc caacaacggc tatctgtacc
     3301 gggtggatat taagggcagc tccccctatg tggtgcaggc cgccgaccta ataaaaccgc
     3361 ccactgacaa acctgccacg ccacccacca gcagcgacta ttttttcacc caattgggct
     3421 atgacccaga acagattggc cgccgcctag gcgacagctt ttatgaaacc gagctggtgc
     3481 gccaagcggt aatgaacacg cgaggtgtgc gctttgtaac cgacccgcgc accggcctcg
     3541 ccattgattc cgacgaagcc caataccgct acctattgga taacgccctc gaagcgcaaa
     3601 aagacctgca attagccgta ggcaccgccc tgacacccga gcaagtgcgt gcattaaccc
     3661 aagacatcgt ctggctggag cgccatgaac tggccgagca agaagtctgg gtacccgtgt
     3721 attacgccac cggcgttgac cccaaccaaa tggccaatgg cgcagtgatg gccgggcaat
     3781 cggtgcgggt ggtgagtgaa gaactgctca atcgaggtga tattcgcggc ctgagcctgg
     3841 tggatgtcag cagtcaaaaa agccaaaact ttggtgccat cggcagcctg gatcgcttaa
     3901 ttgttaacag tcaaagtgtc gataacaaag gcttgatgct gggcaaaaca acccaggtga
     3961 gcggctttga catcgataac agtggtcaga tgatcgcccg cgagaccttg gacgttaccg
     4021 ccgaaaatga actgaacaac cgaggcgaac tggccggtac cgacgtaaat gccaaaagca
     4081 ccggcgcgga tctcatcaac caaggccgca ttaacgccag taataatgct gatctcgacg
     4141 ccgcgcggga tattttgctt aaaccagggc agggggcttc catcaacaca ggcggcaacc
     4201 tctcggccac cgccgggcgc gacttcaacc ttgaaggcca agccatcagc acccaggggg
     4261 acgccaccat cagcgccgcc cgcgatgcag ctatcacacc cgtgactgag cgctcactga
     4321 tcagtggagg tcacaccagc actgaggtca tcactaacac cgccactcgc atcaccaccg
     4381 gtggctcact gagtatcgat gcaggccggg atgttggtat tactgccacc aaaattgaca
     4441 ccgggaaaga tgtcacgctt cgtggtggtc gcgatgtaaa cctcaccagc gctgccgata
     4501 gcacccacac cgaaggccag ggcaaaaact ggcacagcat tgaaaccgac gtaaaacagg
     4561 tcactaccga catcaatgcc gggggcaaga ttgacgccac ggcgggtcga gacatcacca
     4621 tggtggcggt gcagggcagg gcagaagggg acatacacgt caacgccgag cgcgacacca
     4681 acctcctggg cgcggtggat tctcaatacg actacgacta tgagaaaaag aaaaagagct
     4741 tcggccgcag taaaacgacc gaaagtgaaa gcctcaaaga aacggccgta gcgacagaat
     4801 taagcgctgg tggcaatcta ctgctcaata cgaaactggg tagcaacggc gaaattctta
     4861 aggaaaccgg ccagcgcatt aatactgaag gggccaagct cagcgccggt gatcccaaag
     4921 ccctagctca agccccagct caggcccccg atacaccctc aaacaaagca gccgacaaac
     4981 cccaaggcac catcgccgcc tatgccgctg acggcattac cttggcaggc caatccacca
     5041 aaagcctgga ttacagcgaa acccgccgct ccggcacctt ggggctgagc aaaaaagcca
     5101 aaggtagcaa tgaaggcgaa gaccacagca gcgccacaac cctcacctct accggtgata
     5161 ctcagctgct gtcggaccac aatctcaccc ttgaaggggt ggtggccaat gttaacggcg
     5221 acatgacgct gtcggcggtg gacgatctgc gagtagcccc aggcgaaacc cacacacaaa
     5281 cccagacctg ggacaacaaa tccggtgcct tcagcggtgg cagcctgtac cgcaaaaccg
     5341 aaacgaacag cgaagacggt gaaaccggct taaccgacag cgcgctgctc gttaaaggca
     5401 acctcaaagt cgatgccggt agcacggaaa tacttggcac ccgcatccaa accggcagcc
     5461 gagacgaaaa cggcaacttc accgccacca ccgacagcgg caacctcacc cttggcgccg
     5521 cgcaggcctc caagttccac aaagacagca cgctagatgt gcgcctcaaa ggcacggata
     5581 ctctcaaggc cctaaaagaa ccccataaaa tcgttgaaaa cgacgatggc cgcgccaaga
     5641 tcaaactcgg cagcgccacc tacctcaacg ccgacaactc aagcaccacc ctcagccaac
     5701 aaggtactga agtcagcacc ttcggtgaca ttaccctcga tgccaccagc atcggcggga
     5761 aaactaacct agctggcagc accctcaatt ccaccggtat catcgaccta cacggcaccc
     5821 agagcctggc cctcagcagc gtggatgcca cccaaagcga acaacacaaa acccaagaag
     5881 gtaagggcga agccagcttt gtggtgcagc atcaagccgt tgaagtcgcc aaagccgcaa
     5941 agaacgtgct cgacgccaag gaacaactga gtgacgccaa gcgccaactc aaacactacg
     6001 aaaacaacct cgatacccag caaagccagc tcaatcaact cagagccgac ctggctgcgg
     6061 gcacccccgg cgttacggcg gacgatgtac aactgctcag tgaactggtg cgcgaccttg
     6121 aggacgaccg cgcctggtat caaaccggcc tcgccctagc t
//
LOCUS       DAOQGS010000217         6148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6368_length_6148_cov_3.757427, whole genome shotgun sequence.
ACCESSION   DAOQGS010000217 DAOQGS010000000
VERSION     DAOQGS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6368_length_6148_cov_3.757427"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="tssG"
                     /locus_tag="PKZ52_09450"
     CDS             <1..929
                     /gene="tssG"
                     /locus_tag="PKZ52_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNG60040.1"
                     /translation="RLSSHQSLGIPCGDIFRLELEKLSGNQVRWLVVINFLGLTGAIG
                     VLPFHYSELIFERLKLRDNTLKQFFDIFNHRLASLFFQASIKYRLPINYERQHLIPAR
                     KQEHDSITHCLLSLVGLSTPHLEKNTRLNSLLFLRYAGFFTQRVRTATGLKNMIAAYF
                     GVPVQVEEFVGQWQDLIDDVRSRLPDMHNRKGQNACLGRSALIGSKAWFAQGKVRIVL
                     GPLNKQEFYQFAPGTKALAHLHEMVKLYAGMEQDFEYVISVARKHLPYQIQLNQKEPP
                     IVGWDTWLATKPLASYRHGETLNIAISAGRLD"
     gene            1107..3785
                     /gene="tssH"
                     /locus_tag="PKZ52_09455"
     CDS             1107..3785
                     /gene="tssH"
                     /locus_tag="PKZ52_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HNG60041.1"
                     /translation="MINIDLKALVGKLNEPSRQALEGAAGLCLNRTHYNVEVEHWLLK
                     LIEMTDSDIQAILIKYEVNLGKLARDLNQELDRIKSGNTRAPALSPTIVDLAKNAWML
                     ASVEYGHGIATSGHLLAALLLDEGLRRTAEGVSAELKKITPESIRAFARTIIGTTGES
                     KQNLQGDTSSSDDGAPATGAPSKTPALDKFTVNLTEAAKNGKIDAVLGRDEEIRQIID
                     ILTRRRQNNPILTGEAGVGKTAVVEGFALRIATGDVPDPLKNVTVRSLDLGLLQAGAS
                     VKGEFENRLKSVIAEVKASAEPIIMFIDEAHTLIGAGGKEGQGDAANLLKPALARGEL
                     RTIAATTWAEYKKYFERDPALTRRFQVVKVEEPSEDKAIDMMRAIAGMLQNHHKVRIL
                     DEAVIDSVRLSARYITARQLPDKSVSLLDTACARVGLSQSTTPASIEDIRRRIQQIDT
                     NIATLERENATTGNCDEQIAKLYAELTEQQAALATSEAQWNTEKELVAKIHEIQNKID
                     ADFKLAKKSGADAEGRLTEAERLDAKAQLQVLFKELEAVQGENPLIQVNVGSQAIAEV
                     VANWTGIPVGKMVSNEIQQVLNLAEVLGERVIGQQHALEAIAQAIRTSRAGLTDARKP
                     IGVFFMVGSSGVGKTETALALADILYGGEQNITTINMSEFKEEHKVSMLLGSPPGYVG
                     YGEGGVLTEAVRRKPYSVVLLDEMEKAHPGVQDVFYNLFDKGTIKDGEGRDIDFRNTL
                     IIMTSNAAEEHLRAMIAASEEKPDPEVLLDNFRPQLLRYFKPAFLGRTTVIPYYPLGD
                     EDLLKICKINMRRIEKRVKAHYNASFSYDEDVLLHIVARSQEVDTGARNIENILTRTL
                     LPALATECLGKMALGEDIKKVHVGVDEEGVFSYLVE"
     gene            3841..4566
                     /locus_tag="PKZ52_09460"
     CDS             3841..4566
                     /locus_tag="PKZ52_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HNG60042.1"
                     /translation="MEKFMAITLRNGSSGDDVKRLQMQLNSILKLNPPLTADGKFGLK
                     TERAVQAFQAQNKLGVDGVAGPKTQAALNAKLSGKPIQPPPPSPALSPATNSGAAWFE
                     IAKNEMGVHEKAGAAANPRILEYFGSTTLRTTSDETAWCAAFVNWCLAQVGITGTRSA
                     AAASWISWGISKGAQTGAITVIHNPAMANSSLSRSGNHVGFLVEETSTHYVLIGGNQG
                     DEVRKSLFPKSSWQLKGHRWPNV"
     gene            4576..5019
                     /locus_tag="PKZ52_09465"
     CDS             4576..5019
                     /locus_tag="PKZ52_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60043.1"
                     /translation="MNIFSLLVKTFILLVLIGGCATAKEPDNSSFNEFWGQFRQVLLD
                     KDFKKIQSLSANPITLRGVVDGIKPQKLPSQDIEKTLPKILEGRTSYMEAGKIIDLTN
                     YDFVKRTSEPKLDVNSPNWQRVDNFVFIKDKTGWKLKEIFWEEME"
     gene            5170..5709
                     /locus_tag="PKZ52_09470"
     CDS             5170..5709
                     /locus_tag="PKZ52_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60044.1"
                     /translation="MKSEKIGTDMNSESLFTKCKFCGKSIQKTFKKCPSCGKSLIHIT
                     LMHWSGTALISLIVYYYFNTPTPQARTEKESIAIRKMLAIDNIKLEYQWKKEGFGNIM
                     AGDFTITNKNEFSFKDLEIKCINYSKSGTELDSNTRIIYDIFEAKTSKRHTNFNMGFI
                     HEQTSESICHIRNLSLIHL"
     gene            5732..6064
                     /locus_tag="PKZ52_09475"
     CDS             5732..6064
                     /locus_tag="PKZ52_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNG60045.1"
                     /translation="MPLINCPECSAEVSDSALKCMKCGVQLRKAKRGFFGNLFKWTFI
                     VFNFLMAWWLIGGMHAATSAMESTTGAGHVGAAIGTGIGAFMIIGLWVFGDIILGLFV
                     LFTRPKNS"
BASE COUNT         1807 a         1562 c         1385 g         1394 t
ORIGIN      
        1 cccgcctctc cagccatcaa agcctcggca ttccctgcgg cgacattttc cgcctagagc
       61 tggaaaaact ctccggcaac caagtgcgct ggttagtcgt tattaatttt ttaggcctta
      121 ccggcgccat aggggtttta ccctttcact acagcgaatt aattttcgag cgcttaaagc
      181 tgcgcgataa caccctcaaa caattttttg atatttttaa ccaccgcctc gcctcgctgt
      241 ttttccaagc cagtattaaa taccgcctgc ccatcaatta tgaacgccaa catttaatcc
      301 ccgcccgcaa acaagaacac gacagcatca cccactgttt gctctcgtta gtcggtttaa
      361 gcacccctca cctggaaaaa aacacccgct taaacagctt gttatttttg cgctacgccg
      421 gttttttcac ccagcgcgtg cgcaccgcca ccgggcttaa aaatatgatt gccgcttatt
      481 ttggtgtgcc agtacaggtg gaagaatttg tcggccaatg gcaggattta atcgacgacg
      541 tgcgctcgcg cctgccggat atgcacaacc gcaagggcca aaatgcctgc ttgggccgct
      601 ccgccctcat cggctctaaa gcctggtttg cccagggcaa ggtgcgcatt gtgctggggc
      661 cgctgaataa acaagaattt taccaattcg cccccggcac caaagccctc gcccatttgc
      721 atgagatggt caaactctac gccggtatgg agcaggattt tgaatacgta attagcgtgg
      781 cccgcaaaca cctgccctac caaattcaac tcaaccaaaa agaaccgcct attgtcggct
      841 gggatacctg gcttgctacc aaacccttag ccagttaccg ccatggcgag actctaaaca
      901 ttgctatttc cgcaggcagg ctcgattagc ctgggcgcgg tattaaccgg ataaacaacg
      961 ggtacaggac acccacagga gtaatgagca caggccttaa gcaagccggg gcgcaggccc
     1021 caagccagtt tattttgcgc acctgagggt gcatactcag ccgcgatttc accaatttat
     1081 ttaactgcaa cctaaggatc actcctatga tcaacatcga cctaaaagcc ctcgtgggca
     1141 aactcaatga gcccagccgc caggcccttg aaggtgccgc aggcctgtgt ttgaaccgca
     1201 cccattacaa cgtggaggta gaacactggt tattaaaatt aattgaaatg accgacagcg
     1261 acatccaagc cattttaatt aaatacgaag tgaacctagg taaactcgca cgggatttaa
     1321 accaagagct ggaccgcatt aaatccggca atacccgcgc ccccgccctc tcccccacca
     1381 ttgtggattt agccaaaaac gcctggatgc tcgcctcggt agaatacggc cacggcatag
     1441 ccacctcagg ccatttatta gccgctttat tattagatga aggcctgcgc cgcaccgctg
     1501 aaggcgtatc ggcggaattg aaaaaaatca cccccgaatc catccgcgcc ttcgcccgca
     1561 ccatcatcgg caccaccggt gaatccaaac aaaatctaca gggcgatacc agcagcagcg
     1621 acgacggcgc ccctgcaacc ggcgccccca gcaaaacccc cgcattggat aaattcaccg
     1681 ttaacctcac cgaagcggcc aaaaacggca aaattgatgc ggtattaggc cgcgatgaag
     1741 aaatccgcca gattatcgat atcctcaccc gccgccgcca aaacaaccca attcttaccg
     1801 gtgaggcagg cgtaggtaaa accgctgtag tagaaggctt tgccctgcgc atcgccaccg
     1861 gcgacgtgcc ggacccatta aaaaatgtca ccgtgcgcag cctggattta ggcctattac
     1921 aagccggtgc cagtgtaaaa ggtgaatttg aaaaccgctt aaaatccgta atagctgaag
     1981 ttaaagccag cgctgaaccg attattatgt ttatcgacga agcccacacc ctcattggcg
     2041 ccggtggcaa agaaggccaa ggcgatgccg ccaatttatt aaagcctgcg ctggcccgcg
     2101 gtgaattgcg caccatagcc gccaccacct gggcggaata taaaaaatac ttcgagcgcg
     2161 acccagcctt aacccgccgc ttccaagtgg taaaagtaga agagcccagc gaagataaag
     2221 ccatcgacat gatgcgcgcc atcgccggta tgctgcaaaa ccaccataaa gtgcgcattt
     2281 tggatgaagc cgtaatcgac tccgtgcgcc tctccgcccg ttatatcacc gcccgccaac
     2341 tgccagataa atccgtaagc ctattggaca ccgcctgcgc ccgtgtaggc ttaagccaaa
     2401 gcacaacacc tgcaagcatt gaagatatcc gccgccgcat ccaacaaatt gataccaata
     2461 ttgcaaccct ggagcgggaa aacgccacca ccggcaattg cgacgaacaa atcgctaaat
     2521 tatatgccga actcaccgaa caacaagcgg cgctggccac cagcgaagcc cagtggaata
     2581 cggaaaaaga attagtagcc aaaattcacg aaattcaaaa taaaattgat gcggatttta
     2641 aactggcgaa aaaatccgga gccgatgccg aaggccgttt aactgaagcc gaacgcctgg
     2701 acgccaaagc ccaattacaa gtgttattta aagagttaga agcggtacag ggcgagaacc
     2761 cattaatcca agtaaatgta ggcagccagg ccatagccga ggtagttgcc aactggacgg
     2821 gaattcctgt cggcaaaatg gtttccaacg aaattcaaca ggtgttgaat ttagccgaag
     2881 tattaggcga gcgcgtgatc ggccaacaac acgcattaga agctattgcc caagccatcc
     2941 gcaccagccg cgcaggcctt accgatgcgc gcaaacccat aggggtattt tttatggtgg
     3001 gctcatccgg tgtaggtaaa accgaaaccg cattggccct tgccgatatc ctctacggcg
     3061 gtgagcaaaa cattaccacc attaatatgt cggaatttaa agaagagcat aaagtctcca
     3121 tgctgctcgg ctcccctccg ggttatgtag gctacggtga aggcggtgta ctcaccgaag
     3181 ccgtgcgccg caaaccttac tccgtagttt tattagacga aatggaaaaa gcccaccccg
     3241 gcgtgcagga tgttttttat aatttattcg ataaaggcac cattaaagat ggcgaaggac
     3301 gcgatataga ttttagaaat actctaataa tcatgaccag caacgccgcc gaagagcacc
     3361 tgcgcgccat gattgccgcc agcgaagaaa aaccagaccc agaagtatta ctggacaact
     3421 tccgcccaca attactgcgc tacttcaaac ccgccttttt aggccgcacc acggtaatcc
     3481 cttattaccc actgggcgat gaagacctat taaaaatctg caaaattaat atgcgcagaa
     3541 ttgagaaacg cgttaaagcc cattacaacg ccagctttag ttatgatgaa gatgtgttgc
     3601 tgcatattgt ggctcgctcg caggaagtag ataccggggc tcgtaatatt gagaatatcc
     3661 taactcgaac tttgttgcct gcgctggcta ctgagtgttt gggtaagatg gcattggggg
     3721 aagatattaa gaaggtgcat gtgggggttg atgaggaggg ggtgtttagt tacctagtag
     3781 aatgattttt taaccgtcag gcggcccata aatgccgcct gattttttat cagtacaatc
     3841 atggagaaat ttatggcaat cacattaaga aacggttctt caggcgatga tgttaaacgc
     3901 ctacaaatgc aactcaattc aattttaaaa ctcaacccac cgttaactgc cgatggcaaa
     3961 tttggtctaa aaaccgagcg tgcggtgcag gcttttcaag cacaaaataa attaggtgtt
     4021 gatggtgttg cagggccaaa aacccaagcg gccctcaatg cgaaactcag tggcaagccc
     4081 atccaaccac ctccaccctc tcctgccttg tcgccagcaa ccaattccgg tgcagcatgg
     4141 tttgagattg ctaaaaacga aatgggtgtt catgaaaaag ctggcgctgc agctaaccct
     4201 cgcatattgg aatacttcgg atccaccacc cttagaacca cttcagatga aaccgcttgg
     4261 tgtgccgctt tcgttaattg gtgcctagcg caagtcggca taacaggtac acgcagcgcg
     4321 gcggcggcaa gctggattag ttggggcatt agtaaaggcg ctcaaactgg tgcgattact
     4381 gttattcata accccgccat ggcgaatagc tcactgagtc gcagcggtaa tcatgtgggg
     4441 tttttggtag aagagacatc aactcattat gttttgattg gcggcaacca gggtgatgaa
     4501 gtgaggaaat cacttttccc caaaagctct tggcaactta aaggccatcg ctggccaaat
     4561 gtataggaac cctacatgaa tatttttagc cttttagtga aaacatttat attactcgtc
     4621 ctcattggcg gatgcgccac agcaaaagag ccagacaact cttcatttaa tgaattctgg
     4681 gggcaatttc gccaggtttt actggataaa gactttaaaa aaatacaatc tctaagcgct
     4741 aatccaatta ccttgcgcgg cgtagtggat ggtataaaac cccaaaaatt accctcacaa
     4801 gatattgaga aaaccctccc caaaatttta gaaggcagaa caagctatat ggaggcaggc
     4861 aaaattatcg acctgaccaa ttatgacttc gtcaaacgta caagcgagcc gaagctggat
     4921 gtcaacagcc ccaactggca gagggtagac aattttgtat ttataaaaga taaaaccggc
     4981 tggaagctga aagagatttt ttgggaggag atggagtagt ttatgatgat ggccgtaggt
     5041 tgggcagtaa tgcccaacaa aatttgcatc agcaaaatta tttcctgggg cgccaggtgc
     5101 gatattgggc attgctgcct aacctaagag cttccatacg agccaaagct tcccttcact
     5161 gaccgccaca tgaaaagcga aaaaatagga acagacatga acagcgaaag cctattcaca
     5221 aaatgcaaat tttgtggaaa aagcatacaa aaaacattta aaaaatgtcc ttcctgcggt
     5281 aagtctttaa tacacattac ccttatgcat tggtccggaa cagctctaat ttcactaatt
     5341 gtatactatt attttaatac ccccaccccg caggcaagaa ccgaaaagga gagcattgcc
     5401 ataagaaaga tgctagccat agataatatc aagctcgaat accagtggaa aaaagaagga
     5461 tttgggaaca tcatggctgg agacttcacg attacaaaca aaaatgaatt ctcattcaaa
     5521 gatcttgaga ttaaatgcat caattactcc aagagcggaa cagaattaga ttccaacacc
     5581 aggattattt acgatatttt tgaggcaaaa acctcgaaaa gacacaccaa ctttaacatg
     5641 ggcttcatac acgaacaaac cagcgaatca atttgtcaca ttagaaatct ttcattaatc
     5701 catctataga agccttatac aggagaacaa aatgcctttg ataaattgcc ccgaatgctc
     5761 tgcagaagtt agtgattcag cattgaaatg catgaaatgt ggcgttcaac tacgaaaagc
     5821 aaagcgagga ttttttggaa atttatttaa gtggactttc attgtattta attttttaat
     5881 ggcgtggtgg ctgattggag gcatgcatgc ggcaacatcg gccatggaaa gcacaacagg
     5941 cgcaggccac gtaggtgcag cgataggaac aggcattggc gccttcatga taattgggct
     6001 ctgggtattc ggagatataa ttcttggatt atttgtatta ttcactcgac caaaaaactc
     6061 ctagcagtgt ccatcgacat tcgattcccc gttaagatcg aaaaataaaa tcaacgcgca
     6121 ccccggaata gtttttgggc gtgcgtaa
//
LOCUS       DAOQGS010000218         6143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6381_length_6143_cov_4.474540, whole genome shotgun sequence.
ACCESSION   DAOQGS010000218 DAOQGS010000000
VERSION     DAOQGS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6381_length_6143_cov_4.474540"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1057)
                     /locus_tag="PKZ52_09480"
     CDS             complement(176..1057)
                     /locus_tag="PKZ52_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG60046.1"
                     /translation="MINKFSSALVLQMALAFGGQLLLSTHAAAEDAAPPAAPAVETPV
                     AGPQETPPAAAPAPDNAVDAAQEATKEAAHEYGLTALWQQGDIIAKGNLVIMVIMSAF
                     TWYILATKLWQQQTLYGQYKASRKLWEAKSLPEGINSLKSDSAFRMVAEDGVASYKQH
                     EARQGEKIHLGEWIHMAISRSIASVTSDLQTGMAFLATVGSVSPFVGLFGTVWGIYHA
                     LISIAASGQASIDKVAGPVGEALIMTAVGLAVAVPAVLGYNWLTRRNKLAIEQLNSFG
                     SDVHAYLSMGVRGGQKD"
     gene            complement(1126..1743)
                     /locus_tag="PKZ52_09485"
     CDS             complement(1126..1743)
                     /locus_tag="PKZ52_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG60047.1"
                     /translation="MSYHSKKSFASRFSGLIIVVGLHVGAVIALMFGLKPKDEMVIVE
                     NVKVETMEEQKEEVEPPPPPPPDYVPPPPDFAPPPSFNVAADAPAPANAITTAPVKPV
                     EAAPVAKAPPTPAKPPKKGLSPPAYPSESKKLGEEGPVGLALYLNEEGKVQEARVETS
                     SGFPRLDEAAVKQATKAWKFEPCTEDGKPVACWYKIKFRFQLKDA"
     gene            complement(2253..4616)
                     /locus_tag="PKZ52_09490"
     CDS             complement(2253..4616)
                     /locus_tag="PKZ52_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NdvB protein"
                     /protein_id="HNG60048.1"
                     /translation="MLRPTHQGERFELDNPLDMPRAAGFLWNRRMMVQITCRGYAIAQ
                     YMQPEPAKYAYAPNLEAKTFMQPEQPYYAHHPGRFFYIKDETTGALFSAPYEPVRAKP
                     DSFKFSVGQADIQWEIEKDGIAIRLSLTLPSEDVAELWKVEVTNRGDQARSISLYSYF
                     PIGYMSWMNQGAQYRPDLGGIVASCVTPYQKVADHFKNKYFKDKTFFIADRTPTAWEA
                     KQEAFEGEGGLHNPTGTQGQTLDNGDALYETPAAVLQYRLELQAQQTETLHFIFGPAF
                     DDAEIQALTGKYLGAANYAKTLASYSDYIAQGKGCLRIDTPDAELNNFVNHWLARQVF
                     YHGDVNRLTTDPQTRNYLQDNMGMSFIKPDMARRAFLHALSQQEASGAMPDGILIAEG
                     AELKYINQIPHTDHCVWLPVCLRAYLDETNDYAILSEQVTDVEGHSRSVLERISQAMH
                     WLLSARDERGLSYIAQGDWCDPMNMVGYKGKGVSGWLSVATAYALNLWADICSEAGHA
                     SLAPGFRAGADEVNQAVNSHLWDGEWFGRGITDDGVIFGVSRDTEGRIFLNPQSWAIL
                     GGAASKEQQQIMLQAVEAQLETPYGVMMYAPAYTAMRDDVGRVTQKFPGSAENGSVYN
                     HAAVFYIYSLYTIGEQDRAYKLIRQMIPGPSEADYKQRGQLPVYIPNYYRGAYYQHPR
                     TAGRSSQLFNTGTVSWVYRCLVEGLFGVKGQGADLVINPQLPSHWSSAKIERRFRGAL
                     FDIEYCRVKGLDALRVLLNGNPLPNNRIPQPTPGERYQVRVELPAAQ"
     gene            complement(4656..6050)
                     /locus_tag="PKZ52_09495"
     CDS             complement(4656..6050)
                     /locus_tag="PKZ52_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60049.1"
                     /translation="MHKSPQSAQDQRVSWGEKISYGLADTGFNFYWTNISTFLLFFYT
                     EVFGITAAAAGFMMTTVKLINAFTDPLIGAFADRTSTRHGKFRPFLVWMAAPLTVSAV
                     LVYTTPNLGSDGKIIWAYGTYLLMMVCYTCINIPYNALSGVMSADPQQRADINGLRFV
                     GGFTGGTLVSAATPKLVELLGQGDTQVGWQLTMVFWGLAASILFVNAFFNTKERVEAV
                     NEEKTKVWDDIKDLKGNQPWMILFVLALIIMVTITLRQTTAPYYFKYYVERSDLIAAF
                     VPAYMFSAAAGAALTPILTRLIDKRNLMIILMTLTGVFSVAFYFVPKDQVEIMFGLQI
                     AIGLVLGPKSPLAFSMYADTADYNEWRTGRRATAMTFAAATFSQKLGTALATALIGSV
                     FTALGYVANADQSQASLDGIVLLMSIFPAIFAFIAAFVLMFYTLDKQQLKTIQEELTQ
                     RKADKQAASGPILY"
BASE COUNT         1405 a         1463 c         1774 g         1501 t
ORIGIN      
        1 ctacaggtac tgtgatcaaa aagatgatca gtaccaccag catgatatct acgagcggcg
       61 tagtgttgat ggtcgagtta aagtcatcat cattgccgcc aacattcatt gacattgcgt
      121 actacctcgg ttgatgaatt taaagagggg agccattggc tccccaatta tttaattagt
      181 ctttttgacc gccgcgcaca cccatggaca ggtaagcgtg tacatcagaa ccgaagctgt
      241 ttaactgctc aatagccagt ttgttgcggc gagttaacca gttgtaaccc aataccgcag
      301 gtaccgctac ggccagacct acagcggtca tgatcaaggc ttcacctacg gggcccgcta
      361 ctttgtcgat actggcttgg ccggaagcgg cgatgctaat caaggcgtgg taaatacccc
      421 acacagtacc gaacagacct acgaacggag acacagaacc tacagtcgcc aagaaggcca
      481 tgccagtttg taaatcagag gttacgcttg cgattgagcg gctgatggcc atgtggatcc
      541 actcgcccaa gtgaattttc tcgccttggc gggcttcgtg ttgcttgtag gaagctacgc
      601 cgtcttcggc aaccatacgg aaagcgctat cgcttttcag gctgttgatg ccttcgggca
      661 ggcttttggc ttcccacaat ttgcgagacg ctttgtattg tccgtacaga gtttgttgtt
      721 gccacagttt ggtggccagg atgtaccagg tgaatgcaga catgatcacc atgataacga
      781 ggttaccttt ggcgataatg tcgccttgtt gccatagagc tgttaagccg tattcgtgag
      841 ccgcttcttt ggtggcttct tgagctgcgt ctacagcgtt atcgggcgct ggggctgcag
      901 caggaggagt ttcctgtggg cctgcaacag gagtctcaac cgcaggagca gctggtggcg
      961 ctgcatcttc agcagcagcg tgagtagaaa gcagtagttg accaccaaag gccaatgcca
     1021 tctgcaatac aagtgcggag gaaaatttat taatcattgt taaatcctac aattaacaga
     1081 gggggtcatt aaatttagag ctaaagcctg cggcccagca ccgggttatg cgtctttaag
     1141 ttggaagcga aacttaattt tgtaccaaca agcgacgggt ttgccgtctt ccgtgcaggg
     1201 ctcaaatttc catgctttag ttgcctgttt tacagcagct tcatctaacc gagggaaacc
     1261 gcttgatgtc tccacacgcg cttcttgcac cttgccttct tcgttcaaat acagcgcaag
     1321 tccaaccggg ccttcttcgc cgagtttttt agattcacta ggataagcag gcgggctaag
     1381 gcctttttta ggtggtttgg caggcgttgg aggagcttta gcaacgggtg ctgcctctac
     1441 cggttttact ggagccgtgg taatagcgtt ggcgggggca ggcgcatcag cggcgacgtt
     1501 gaaggacggc gggggggcaa aatccggtgg aggtggtacg taatccggtg gtggtggcgg
     1561 tggtggttca acctcttcct tttgttcttc catggtttca acttttacgt tttctacaat
     1621 taccatttcg tccttgggct taaggccaaa catcaacgcg atgacggcac caacgtgcaa
     1681 gcccacaacg ataatcaacc ctgagaatcg tgaggcaaag ctcttcttcg agtgatatga
     1741 cataacctaa gtcgccacct tataaatcaa gatttgtcaa aaactagcac actcctctcc
     1801 cacaccggtg aaccgtgagc gaggtgaatt gaagtgatac cagaatgggg ttctaacacg
     1861 cagattttta gcaaaagggc ttaagccaga tggcgaatta aaaatcgacg tttttgctgc
     1921 agttgaacat ttattggtta ttaatcaggt gcttcgcggc acccattcca actgagcggg
     1981 gtcgcattgt agcgtcagta ttcaggcatg aaaagtctta tttgcattta atttacccgc
     2041 atgaggcccg tgtttattgg ggttcgcgcc gatctttagc cttaggtaag cggttgcgaa
     2101 cacacttaaa ggtatagatt tagcaccggc tattggcaag cgccaaaagg ctttttgaga
     2161 aaaattaaat cgccgtacac ctttattttt ctggtcttta gataaaaata aaaacgcgtt
     2221 aataaaggtg gcggcgacga tttgcgacgg ttttattgcg cagcgggcag ttcaacccgt
     2281 acctgatagc gctctccagg tgtgggttgg ggtatgcggt tattgggcag agggttaccg
     2341 tttagtaaga ctcgcagggc atcaagccct ttgacacggc agtattcgat atcaaataga
     2401 gcgcctcgga agcgtcgttc aattttggcg ctggaccagt ggctgggcaa ttgagggttg
     2461 atgaccagat ctgcgccctg gcctttgaca ccgaataaac cttcaaccag gcagcgatac
     2521 acccaagaaa ccgtcccggt gttaaacagt tgactggagc ggccggcggt gcgcgggtgt
     2581 tgatagtagg cgccgcgata gtagttgggt atgtacacgg gtaattggcc gcgctgcttg
     2641 taatcagctt cgctggggcc aggaatcatt tggcgaatca gcttataagc gcgatcctgt
     2701 tcgccgatgg tgtacagact gtagatgtaa aacacggcgg cgtggttgta caccgagccg
     2761 ttttcagcgg agccggggaa tttttgtgtc acccggccta catcatcgcg catggcggtg
     2821 taggcggggg catacatcat gacgccatag ggtgtttcca actgcgcttc aaccgcttgc
     2881 agcataattt gctgctgctc tttactcgca gcgccgccta ggatggccca gctttgcggg
     2941 ttgaggaaaa tacgaccttc ggtatcgcgg ctgacgccaa agattacgcc atcatcggtg
     3001 atgccgcggc cgaaccattc gccgtcccaa aggtggctat tgaccgcttg gttgacttca
     3061 tctgcacccg ccctaaagcc aggggcgagg gatgcgtgac cggcttcgct gcatatatct
     3121 gcccatagat taagcgcgta ggcggtggcc acggacaacc aaccggaaac gcctttgccc
     3181 ttgtagccca ccatgttcat ggggtcgcac caatcgccct gggcgatata acttaagccg
     3241 cgctcatcgc gggcactcag cagccagtgc atagcctggc tgattctctc cagcacgctg
     3301 cggctgtggc cttcaacgtc ggtaacctgt tcgctgagga tggcgtaatc gttggtttca
     3361 tccaggtagg cgcgcaggca aaccggcagc cagacgcagt ggtcggtgtg gggaatttgg
     3421 ttgatgtatt taagctctgc accctcggcg atgaggatgc catcgggcat ggcgccgctg
     3481 gcctcctgtt ggcttagggc gtgtaaaaag gcgcggcggg ccatatcggg cttgatgaaa
     3541 ctcatgccca tattgtcttg caggtagttg cgggtttgcg ggtcggtggt gagacggttg
     3601 acgtcgccgt ggtagaacac ctggcgggca agccaatggt tgacgaagtt attcagctcc
     3661 gcatccggcg tatcgatgcg caaacaaccc ttgccctggg cgatgtaatc gctgtagctg
     3721 gccagggttt tagcgtaatt agcagcaccc agatacttgc cggttagagc ttgaatttcc
     3781 gcgtcgtcaa aggccgggcc aaatataaaa tgcagggttt cggtttgctg cgcctgcagc
     3841 tcaaggcggt actgcagtac cgctgcaggc gtttcataga gggcatcgcc gttgtcgagg
     3901 gtttggcctt gggtgccggt ggggttgtgc aggccacctt cgccttcaaa ggcttcttgt
     3961 ttggcttccc aggcggtggg tgtgcggtcg gcgataaaaa acgttttgtc tttgaagtat
     4021 ttgtttttga agtggtcggc cactttttgg tagggcgtta cacagctggc cactatgccg
     4081 cctaaatctg ggcggtattg ggcgccctgg ttcatccagc tcatatagcc gatggggaaa
     4141 taactataaa ggctgatgga tcgggcttga tcgccgcgat tggtgacttc aactttccaa
     4201 agctccgcca cgtcctccga ggggagtgtc agagagaggc gtatggcaat accgtctttt
     4261 tcgatttccc actgtatgtc ggcctgcccg acggagaatt taaaagagtc aggtttggcg
     4321 cgaaccggtt cgtagggggc ggagaagagg gcgccagtgg tttcatcttt gatgtagaaa
     4381 aaacgcccag ggtgatgggc ataatagggc tgttccggct gcatgaaggt tttagcttca
     4441 agattggggg cataggcgta tttggccggt tccggctgca tatattgggc aatggcgtag
     4501 ccgcggcagg tgatttgtac catcatccgg cggttccaca ggaagcccgc agcgcggggc
     4561 atgtctaaag gattgtcgag ttcaaagcgc tcgccctggt gggtgggtcg taacatgggg
     4621 gctcctcggg atcaggatat tagctgtgcg tggtgttaat aaagtatcgg cccgctggcg
     4681 gcctgtttgt cggctttgcg ctgggttaat tcttcttgaa tggttttgag ttgctgcttg
     4741 tcgagggtat aaaacatcaa tacaaaggcg gcaataaagg caaaaatggc tgggaatatg
     4801 gacatcaaca acacaatacc atccagggat gcctggcttt ggtcggcgtt ggccacatag
     4861 cccagggcgg tgaagacgga gccgataagc gcagtggcga gtgccgtgcc gagtttttgc
     4921 gaaaaggtgg cggctgcgaa ggtcatggcg gtggccctgc ggcctgtgcg ccattcgttg
     4981 taatcggcgg tatcggcgta catggagaaa gccaatgggg atttaggccc tagcactagg
     5041 ccgatggcaa tctgcaagcc gaacataatt tctacctgat ccttcggtac aaaataaaag
     5101 gcgacggaaa aaaccccggt tagggtcatc aggatgatca tgagattgcg cttgtcgatt
     5161 aagcgggtca ggatgggggt aagggcagcg cctgctgcgg ctgagaacat ataggcgggc
     5221 acaaaggcgg cgattaaatc agatcgctct acatagtatt taaagtagta gggtgccgtg
     5281 gtttggcgca gggtgatggt gaccatgatg atcagtgcca gcacaaacaa aatcatccag
     5341 ggctggttgc ctttaaggtc tttaatatcg tcccaaactt tggttttttc ttcatttact
     5401 gcttcaacgc gctctttagt attaaaaaac gcattgacga acaaaatact ggccgccagg
     5461 ccccagaaca ccatggtcaa ctgccagccc acctgggtgt ccccctggcc taacaattcc
     5521 acaagttttg gtgtggcggc gctcaccaat gtgccgccgg taaagccgcc gacaaaacgc
     5581 aggccattaa tatcggcgcg ctgctgcggg tcggcggaca taacgcccga cagggcgttg
     5641 tagggaatat taatacaggt gtaacacacc atcattaaaa ggtaagtgcc ataagcccag
     5701 atgattttgc cgtcgctacc taaattgggt gtggtgtata ccaacacggc agagacggtg
     5761 agcggcgccg ccatccagac taaaaagggg cggaatttgc cgtggcgggt ggaggtgcgg
     5821 tcggcgaagg cgccgatgag tggatcggta aaggcgttga tgagcttaac cgtggtcatc
     5881 ataaagcccg ccgccgcagc ggtgatgcca aatacttcgg tgtaaaaaaa taataaaaaa
     5941 gtggagatat tggtccagta aaaattaaag ccggtatcag ctagcccata gctgattttt
     6001 tcgccccagg aaacgcgctg atcctgggct gactggggag atttgtgcac agacaactcc
     6061 tgatggttat tgtagttttg ccataaaaaa cactgggggt tttacacgca agcacggccc
     6121 gttaagcggc gccgcaagtg ctt
//
LOCUS       DAOQGS010000219         6107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6425_length_6107_cov_5.206543, whole genome shotgun sequence.
ACCESSION   DAOQGS010000219 DAOQGS010000000
VERSION     DAOQGS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_6107_cov_5.206543"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1597
                     /locus_tag="PKZ52_09500"
     CDS             65..1597
                     /locus_tag="PKZ52_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNG60050.1"
                     /translation="MTCLRLILGDQLNINHSWFAKAQRSRTDLIYVLMEVRSETDYAL
                     HHGQKVLGIFAAMRAFAEALKKAGLSVHYIKLSDPTNQQDFIANIKNLMSRYSITSIE
                     RQQADEYRLEQYLTGLSDLGIAVKVVDSEHFLLRREEAIKRFAHKNTRMEFFYRDLRK
                     QYEILLDDYGAPLGGKWNYDHDNRKRWNGAPTAPQWYVLANDLRSLWQEISEAGVKTL
                     GDPMAQAFPWPISRSQAKAWLAHFCQQSLPYFGEFQDAMSVHSGTLFHAGISFALNIK
                     LLHPLEVIQAALAEFHQGRVSLATIEGFVRQILGWREFVRAIYWARMPAYARHNVLQH
                     TRPLPKWFWDGQTHMQCLQQAITQSLNTAYAHHIQRLMVTGNFALLAGCHPDEVDAWY
                     LGIYIDAFEWVEMPNTRGMSQYADGGILGSKPYVSSASYITKMSDYCKHCHYQANRRH
                     GEGACPLNSLYWHFYARHGDKLSKNPRIGMAYVQWQKMPAEEKQLVLAQAEHYLATID
                     EL"
     gene            1625..2617
                     /locus_tag="PKZ52_09505"
     CDS             1625..2617
                     /locus_tag="PKZ52_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG60051.1"
                     /translation="MNKTYDVIVIGAGMAGLTAANRVQQAGKSVCVLDKSRSFGGRMS
                     TRREGPLYWDHGAQYFTAQSAEFCQQVNTWLQQGVVALWDTPIGAWDGAHLTATEPHL
                     RYVGVPVMKSPLQLLAGELLIYFNQAVRNLLKTPSGWQIKTDAEIFKAKNLVVTLPAP
                     QAAALLPADCAAHALAKRVNMQPCWALMLATQAQVQLPYGGIFINQGPLAWVAADSTK
                     PLRNNAAHTWVIHASAEWSQQQLEKTAAEITPLLIDEFNHLLKTWQPNKPTPDWHPVV
                     AHRWRHARGAVMAATQVWPEESLAIAGDWLSGGKVEGAFLSGLRCGQQLIQAGI"
     gene            2620..3711
                     /locus_tag="PKZ52_09510"
     CDS             2620..3711
                     /locus_tag="PKZ52_09510"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNG60052.1"
                     /translation="MAVSTHTINNRARAALQLLYVADALSMPVHWFYNPLDILKAFPG
                     GIKQFEAAPSLHPSSIMALHSTSSGGRGPQNKTHQREVVGEIILKGKRPFWGIAHQHY
                     HQGMAAGENTLNAHCARLITRSLIAHQGRYSSEDFLQRYIAFMTANTPQHPDTYAESF
                     HRAFFAQYAKGIAAHKCGFKTHDTPSVGGLVMIAPIAISELIREPSLAKTQAICREHL
                     FLTHPDEHLATICDAYVALIHLLLFRDNTPARDIIAAIAKSSVGLNLSQLAQRNLTDA
                     EVVGGLYSKACYIDDAWPSLLFLLYKYLENPPQGLLANTNLGGENCHRGSVLGVLLGL
                     ATEDQWADMFIKLVDAQAIDREIHSLLAD"
     gene            complement(3717..4160)
                     /locus_tag="PKZ52_09515"
     CDS             complement(3717..4160)
                     /locus_tag="PKZ52_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG60053.1"
                     /translation="MKRTDTKSHCPINFTLEILGDPWSLLVLRDMVFFGKQTFKEFLE
                     SSERITTSVLTSRLNNLEKHGIVLKQPHPSDQRSALYRLTEKGIGLVPVLLAMMEWGT
                     RHDPASAGHRKKALVNRIRAEQSAISREVQTTLRKGGAVFVAPQE"
     gene            4389..4802
                     /locus_tag="PKZ52_09520"
     CDS             4389..4802
                     /locus_tag="PKZ52_09520"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNG60054.1"
                     /translation="MTTPYTGGCACGAIRYEINAEPLIGVECHCSDCRHESGNGHASH
                     LVFPRAAARIDGTASEWSMTADSGNIKTRAFCPTCGTPVYMIFAANPAAFSVRAGSLD
                     EPSRYTPKVVTYTSRALAWDMVDSSLPSFTEMPPR"
     gene            4912..5667
                     /locus_tag="PKZ52_09525"
     CDS             4912..5667
                     /locus_tag="PKZ52_09525"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG60055.1"
                     /translation="MQLADSTILITGGSRGLGLGIVEALVAEGAQVSVVARDTTQLEQ
                     IAARVPIDTFSADVTDPLAAAKILSAVAPDVIILNAGAPPKMASLTDISWEEFSHTWD
                     IDVKAGLYWLQAILKNNPAKAQRILVVSSGAAINGSPASGGYAGAKRMLWLMAQYAQG
                     IAEQQHLNKHFQVIVPQQMVLGTGIGDAGARAYAKAAKISPEEFVLRFGAPMAPRDYG
                     DKVAQLLTDERFTQGFAFGISGEDGIRILQPSE"
     gene            5679..>6107
                     /locus_tag="PKZ52_09530"
     CDS             5679..>6107
                     /locus_tag="PKZ52_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNG60056.1"
                     /translation="MQPVIDWTEFLTAAAPLQPMLHKYCARLLGSVTDGEDLLQDTLI
                     KAFTAQNAPTDPQQLRAWLFKIAHNRAIDLLRAKVTKNPYSLEQMTELSVAADYEDSF
                     VSNDADPLQQLIQQETLAITLGAFVQLPIAQRSVVILKDVL"
BASE COUNT         1537 a         1485 c         1534 g         1551 t
ORIGIN      
        1 tgccaaattc gcctagctag tgcttcgccg cctacatttt tcagaggttc cctaaaaaaa
       61 taccatgact tgcctgcgac ttattttggg cgatcaactg aatattaatc acagttggtt
      121 tgccaaggcc caacgcagcc gcaccgacct gatttatgtg ttgatggaag tgcgcagtga
      181 aacggattat gcactgcacc atgggcaaaa agtgctgggt atttttgccg ccatgcgcgc
      241 atttgcagag gcattaaaaa aagccgggct tagtgtgcac tacattaagc tgagcgaccc
      301 gaccaatcag caggatttta ttgccaatat taaaaattta atgtcccgct atagcattac
      361 cagcattgag cggcaacagg ccgatgaata tcgtttggaa cagtatttaa ctggtcttag
      421 cgatttgggg atagccgtta aggttgtgga cagcgaacat tttttgctga ggcgggaaga
      481 ggcaattaaa cgctttgcac acaaaaatac ccgtatggaa tttttttacc gggacctgcg
      541 caaacagtac gaaattttat tagatgatta cggcgctcca ttgggcggta aatggaatta
      601 cgatcacgac aatcgcaagc gctggaatgg cgcgcccaca gcgcctcaat ggtatgtatt
      661 agcaaatgac cttcgctcac tctggcagga gattagtgag gctggtgtaa aaaccctggg
      721 cgaccccatg gcccaggcat ttccctggcc cattagccgc agccaggcta aggcttggct
      781 tgcgcacttt tgccaacaga gtttgccgta ttttggtgaa tttcaagatg ccatgagtgt
      841 gcactcgggc accttatttc atgcgggaat ttcctttgca ctcaatatta aattgctcca
      901 tcccttagaa gtaattcaag cagcattagc agaatttcac caaggccgtg taagccttgc
      961 caccatcgag ggttttgtgc ggcaaatatt gggctggcgg gaatttgtgc gggccattta
     1021 ttgggcccgt atgcctgctt atgcaaggca taatgttttg cagcacacgc gcccattacc
     1081 caaatggttt tgggatgggc aaacccatat gcagtgttta caacaggcca taacccaatc
     1141 cctcaatacg gcttatgccc atcatattca gcgtttgatg gtgacgggca attttgcgct
     1201 gttagccggg tgccatccag acgaggtcga tgcatggtat ttaggtattt atatcgatgc
     1261 ctttgaatgg gtggaaatgc ccaacactcg cggcatgagc caatatgccg atggcggtat
     1321 attaggcagt aaaccctatg tcagctccgc cagttacatc actaaaatga gcgattattg
     1381 taaacactgt cattaccaag ctaatcgccg ccacggcgag ggcgcctgcc cgttgaatag
     1441 tttgtactgg catttttatg cccgccacgg ggataaactc agtaaaaatc cccgcatagg
     1501 catggcctat gtgcagtggc aaaaaatgcc agcagaagaa aagcaattgg ttttagccca
     1561 agccgaacat tatttggcga ccattgatga attgtaggcg cttacaaaaa tgtgaggatt
     1621 cagcgtgaat aaaacctacg atgtcatagt aatcggtgcc ggtatggcag gtttaacggc
     1681 agccaataga gtgcagcagg ctggcaaaag tgtctgtgtg ttggataaat cccgcagctt
     1741 tggcgggcgc atgagtaccc ggcgggaggg gccactgtac tgggatcacg gcgcccaata
     1801 ttttactgcc cagtccgctg aattttgcca gcaggttaat acctggttgc aacagggtgt
     1861 agttgcgctt tgggacacac ccataggcgc ctgggacggt gcgcatttaa ccgctacaga
     1921 accccacctg cgttatgtgg gtgtaccggt gatgaaatca cctttgcaat tattggccgg
     1981 ggaattatta atttatttta accaggcggt gcgcaacctt ctaaaaaccc ctagcggctg
     2041 gcaaattaag acggatgcgg aaatttttaa ggctaaaaat ctagtggtaa cactgcctgc
     2101 cccgcaggca gctgctttat taccggctga ttgcgccgcc cacgctttgg ccaagcgcgt
     2161 taatatgcag ccctgctggg ctttaatgtt ggcaacccaa gctcaagtac aattgcccta
     2221 tgggggaatt tttattaacc aagggccttt agcctgggtg gcggctgaca gcacaaaacc
     2281 tctgcgtaat aatgccgcgc atacctgggt tattcatgcc agtgccgaat ggtcccagca
     2341 gcaattagaa aaaaccgctg cagaaattac ccctttatta atcgacgaat ttaatcacct
     2401 attaaaaacc tggcagccca ataagcctac gcccgattgg cacccggtgg tggcccaccg
     2461 ctggcgccat gcgcgcggtg cggtgatggc cgcaacccag gtatggccgg aggagagttt
     2521 ggctatagcg ggggattggt tatccggcgg caaggtagag ggggcatttt tatcgggcct
     2581 gcgctgcggc caacaattaa ttcaggcggg tatttaatta tggccgtttc cacccacacc
     2641 attaacaacc gcgctcgggc ggctttgcag ttactttatg tggccgatgc tctttccatg
     2701 ccggtgcatt ggttttataa ccctttggat attctaaaag cctttcctgg cggtattaaa
     2761 caatttgaag ccgcacccag tttgcatcct tcatcaatta tggctttaca ttccacctca
     2821 agcggtggac gcggcccgca gaataaaacc catcagcgcg aagtggtggg ggagattatt
     2881 ttaaaaggca aacgcccatt ttggggtatt gcccatcagc attatcacca gggaatggcg
     2941 gcgggggaaa atactttaaa tgcccattgt gcacggctga ttacccgcag tttaattgcc
     3001 catcagggcc gttacagtag cgaagatttt ttacagcgct atatcgcctt tatgacggca
     3061 aataccccac agcatcctga cacctacgcc gagtcatttc atcgggcttt ttttgcccag
     3121 tatgccaaag gaattgccgc ccataaatgc ggctttaaaa cccatgatac cccttcggta
     3181 ggtgggctgg tgatgattgc accgattgcc atcagtgaat taattcgcga gccctccctg
     3241 gcaaaaacac aagccatctg ccgggagcat ttattcctta cccacccgga tgaacacctg
     3301 gcaaccattt gtgatgccta tgtggcttta atccatttat tattatttcg cgataatact
     3361 cccgcccgcg acattattgc cgccattgct aaatcttcag ttggtttaaa tctatcccaa
     3421 ttggcgcagc gcaatttaac cgacgctgaa gtagtggggg gcctttattc caaggcctgt
     3481 tatatagatg atgcctggcc gagcctatta tttttgctct ataaatatct ggaaaatccg
     3541 ccccagggtt tattggccaa caccaatttg ggtggtgaaa attgccaccg aggatcggta
     3601 ttgggtgttt tattggggtt ggcaactgaa gatcagtggg cggatatgtt tataaaacta
     3661 gttgacgcac aagcaatcga tagggaaatt cacagcctgt tggctgatta aattttctat
     3721 tcctggggcg caacaaatac cgcgccgccc ttgcgcaaag tggtttgcac ctcccggctg
     3781 atggcacttt gctccgctct aatacgattc accagggctt tttttctatg ccctgcgctc
     3841 gcaggatcgt gacgagtgcc ccactccatc atcgccaaca atactggcac caggcctatg
     3901 cctttttccg taagcctata taaagcgctg cgctggtcgg aggggtgcgg ttgttttaac
     3961 acaataccgt gtttttccaa attattgagg cggctggtta atacgctggt ggtaatgcgc
     4021 tcggaggatt ctaaaaattc tttaaaggtc tgcttgccaa aaaaaaccat atcccgcagc
     4081 accaacagcg accatgggtc gcccaggatc tcaagggtga agtttatagg gcaatgggat
     4141 ttggtatcgg tgcgtttcat gattgtctta tagatggagg caaatttagt ctaacaaagt
     4201 tacttgcatt atcaaactta gttttgtacc cttgccacaa caagcttgca ttatgcaagc
     4261 taaattagcc tgccagatga cagttctatc ggctaaatca ccagaggcct taatgccgtt
     4321 agtgtttggc tgtaaaaaaa cattatcgaa gcgagtttta tttcccccac ccacacgaga
     4381 ttcagcttat gacaactccc tataccggcg gctgcgcctg cggcgctatt cgttacgaaa
     4441 ttaatgcaga acccctcata ggagttgaat gccattgcag cgattgccgc cacgaaagcg
     4501 gcaacggcca tgcatcccat ttggttttcc cccgcgccgc cgcgcgcatt gacggcaccg
     4561 ccagtgagtg gagcatgaca gccgacagcg gcaatattaa aacccgcgct ttttgtccca
     4621 cctgcggcac accggtgtat atgatctttg cggccaatcc agcggcattt agcgtgcgcg
     4681 caggcagttt agatgagccc agccgctaca caccaaaagt tgtcacctac acatcccgcg
     4741 ccctagcctg ggatatggtg gatagttcac tgccgagttt taccgaaatg ccgccgcgct
     4801 aaatatgggc ttctgctggg ttgactatcg gcaggttcgg caaaaattat cggcactgac
     4861 tttttaaaaa ttgttgagtt ttagctaaac ccatccaacg aggaattaac aatgcaatta
     4921 gccgatagca caatactgat tacaggtggc agccgaggtt tgggccttgg catagttgag
     4981 gcactggttg cagaaggcgc ccaggtaagc gtggttgccc gcgatacaac gcagcttgaa
     5041 caaatcgcag cgcgtgtacc cattgacact tttagcgccg atgttactga cccgctcgcg
     5101 gcggcaaaaa tacttagcgc cgtggcgccg gatgtgatta ttttaaatgc cggtgcgccg
     5161 cctaaaatgg cgagccttac ggatattagc tgggaggaat ttagccacac ctgggacata
     5221 gacgtaaaag ccgggctgta ttggctgcag gccatattaa aaaataaccc cgctaaagcg
     5281 cagcgaatat tagtagtttc cagcggcgct gcaattaatg gatcacctgc ctcaggtggt
     5341 tatgccggtg ccaaacgcat gctttggtta atggcccagt acgctcaggg tattgccgaa
     5401 caacaacact taaataaaca cttccaagta atagtgccgc aacaaatggt gttgggaacg
     5461 ggaattggcg atgcaggtgc cagggcttat gcaaaggctg ccaaaatatc gccagaggag
     5521 tttgtgttgc gttttggcgc gcccatggca ccgcgcgact atggtgataa ggtcgcgcag
     5581 ttattaacgg atgagcgttt tacacaggga tttgcatttg gaattagcgg ggaagatggc
     5641 attcgaattt tgcagcccag tgaataagag gcaagttggt gcaaccggta attgattgga
     5701 cagaattttt aactgccgca gcgccactgc agccaatgct acataaatat tgcgcgcgcc
     5761 tattgggctc ggttacggat ggggaggatt tgctgcagga cacgctgatc aaagcattta
     5821 cggcgcaaaa cgcgcctact gacccacagc agctgcgcgc ctggttattt aaaatcgccc
     5881 acaaccgcgc cattgatcta ttgcgggcta aagttacaaa aaacccttat tcactcgagc
     5941 agatgacgga attatctgtg gctgcagact atgaggatag ttttgttagt aacgatgcgg
     6001 atccgttaca acaattgatc cagcaggaaa ccctggcaat tactctgggc gcttttgtgc
     6061 agttacccat tgcccagcgc agtgttgtga ttttaaaaga cgtattg
//
LOCUS       DAOQGS010000220         6097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6435_length_6097_cov_5.375207, whole genome shotgun sequence.
ACCESSION   DAOQGS010000220 DAOQGS010000000
VERSION     DAOQGS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_6097_cov_5.375207"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..4945
                     /locus_tag="PKZ52_09535"
     CDS             80..4945
                     /locus_tag="PKZ52_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNG60057.1"
                     /translation="MAEPSAPPSAPLAPAEADEETEASTEDDSDLIDDEIIEVFIEEA
                     GEVLATLDEYYPQWLEALDNTELLTVIRRAFHTLKGSGRMVGAQDVGEVAWSVENMLN
                     RVLDNSIEIAATHLDYISRVLAFMPALVDAFASKKRNPDAALTQQLIALGQALARGEV
                     PELASAEAFGAETEAADGDDEQTVLFDLFASEAVTHLAVVDGFIGQMQELAPLFTPPS
                     DSLQRALHTLKGSAHMANVAPVASIATPLEGFTKELRAYQVNLNADMLQLLMDASRYI
                     HGVVDDIKARRALIIPAAEQFIARVHELKELHLDHLIRMKEQVSPDGHKQVDPRLLSI
                     FMAEDMRLLLDADQILARWLHDANEEHTIRDLVQELGRLQAGAAQANLPTMAELSGRL
                     ALLHELALAALPQATAAYVEHLLPAHEFLLDMVDAVAAGQNLPDIPAALQEGLGELNE
                     QLSDMLAAQHRGADEELVLASLEELEAAADGSDHEDEADAAMAHILAEEESAEHDETE
                     QVPVLDIEALQLDHEEFDLPTLDELEAAVAAQESETQESEPLEFETQSQPTEIEDQAF
                     DAEVSAALAAEESVFADEQAQAEPALPEDWAAAEFDSASFDHEALAADTSEPELAATA
                     EELFTLEPDADEAAELVALEAEEHLDPILGDMDLDLVTAIDSHLSADDTLTLLDADAE
                     DMLLPSAEETFAAAVDDDDVIVPLLDELVDLNAEDEPLISALDDFDPIPVVTDSLDLD
                     AIEDGEAGEESEFGLADADDYAASEPYEDEIALGDFDLGEEDLAELLAQTREPAEDAP
                     APVQAATATPAAPVASASFLPEASRDKPASERVEDDFDPEILEIFLEEADELMEELDE
                     SIHAWESDWDSSEAPDVMKRALHTLKGGARLASQIQLGELTHNYESYLIGVGGPKGDV
                     NFFATILAYQDQLLKSIRKSRQLMDGVASEVEESDEAVDAPTLTAKIAEIASTSSPVP
                     FVSSKNVVAVAPEDTKEVVEAFASPVVTNLPDFVARSKSGSQEVIKVSSDLLEELVNL
                     AGETSISRGRIEQQIKGFGAAINEIDGTLRRLQEQLRRLDIETEAQILFRQEQLAENE
                     NFDPLEMDRYSQLQQLSRSLTESASDLVDLKRTLSEKVKDTEAILLQQSRINSSLQEG
                     LMRSRMVPFSRLVPRLRRIVRQVAGELGKHVSFELDNVEGEMDRSVLERMVAPLEHML
                     RNAVDHGIEPPAERLAKGKPEFGRILLSLAREGSDILLRLADDGRGISLDKVRKKAVE
                     RGLMGEHSQLSDHEVMQFILQAGFSTAESVTQISGRGVGMDVVAAEIKQLGGSMTIES
                     RSGVGSQFTVRLPFTVSVNRALMVTLGKDTYAIPLNAIEGIVRVSPFELEHYYQQPEA
                     RFEYADENYQVRYLGGMLYEGTTPKLEGQLLPLPVILVRSAQHTMALQVDSLQGSREI
                     VVKSLGKQFAAVQGLSGATVMGDGSVVVILDVHALVRKAVAFVDALPLNHERTKALNA
                     PRVQKTVMVVDDSVTVRKVTTRFLEREGYLVITAKDGVDALKILQDHTPDLMLLDIEM
                     PRMDGFEVAKNIRTTARWKHIPIVMITSRTGDKHREHALSIGVNEYLGKPYQEDVLAG
                     IVSRILGDKGP"
     gene            4949..5902
                     /locus_tag="PKZ52_09540"
     CDS             4949..5902
                     /locus_tag="PKZ52_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNG60058.1"
                     /translation="MKLTGLAVGVLAESKPQLLDLHRLLAPLPHTLVASQVFSGETLG
                     SLPRADVWLVQLDMDDPAAQSLLDAMDDANLPVIVADEDEAPTDATDPSQWPEVRRRE
                     RVRRLQTKLDLLIDQQPQKAKRATAVWVLAASTGGPEAVGQFLEALDQPPEQLALIYA
                     QHISPSGHKSLVQMLRQRTGLKVCGTHQNQAIEAGCLYVVSPNQALDIVTPGRITPKQ
                     QPWAGRFTPSINQVIAKVARVYGAQGGAVMFTGMGEDGARGCQLLHHLGGQVWIQTPA
                     SSAIDSMPRSVERLGCTRFAGNPRELAQALQRRLNRTQVSV"
BASE COUNT         1272 a         1872 c         1860 g         1093 t
ORIGIN      
        1 cctgccagag cttgcgcccg aggcagccgc cgcgcccgag gctttggctc ctgcgcaagc
       61 gttggcccct gcgtcagctt tggctgagcc gtctgcgccc ccaagcgccc cgctggcccc
      121 ggctgaggca gatgaggaga ctgaggcgag caccgaagac gacagcgatc tgatcgacga
      181 tgaaatcatt gaagtattta tcgaggaggc cggtgaggtg ctggccacct tagatgagta
      241 ctacccccag tggctggaag cgctcgacaa caccgaatta ttaaccgtga tccgccgcgc
      301 ctttcacacc ctcaagggct ctggccgcat ggtgggtgcc caggatgtgg gcgaggtggc
      361 ctggtcggtg gagaacatgc tcaaccgggt gctggataac agcattgaaa ttgccgccac
      421 gcacctggac tacatcagcc gcgtgttggc gtttatgccc gccctggtgg acgcctttgc
      481 cagcaagaag cgcaacccgg atgccgcact cacccagcag ctgattgccc tcggccaggc
      541 cttggcgcgc ggcgaagtgc ccgagttggc cagcgccgag gcatttggcg ccgagacgga
      601 ggctgccgac ggggatgatg aacaaaccgt gttgtttgac ctgtttgcct ctgaggcggt
      661 gacccacttg gccgtagtgg atggattcat cggccagatg caggagttgg cgccgctctt
      721 tacgccgccc tccgacagcc tgcagcgcgc tttgcacact cttaaaggca gtgcccacat
      781 ggccaatgtg gcgccggtgg ccagcatcgc cactccccta gagggcttca ccaaagagct
      841 gcgcgcctat caggtgaacc tcaatgccga catgctgcag ctgttgatgg atgcctcccg
      901 ctatattcac ggggtggtgg atgacatcaa agcccgccgg gccttgatca tccccgccgc
      961 cgagcagttc atcgcccgcg tgcacgaact taaagagctg catctggatc acctgatccg
     1021 catgaaagaa caggtctccc cggatggtca caagcaggtg gatccgcgcc tgttatccat
     1081 ttttatggcc gaggacatgc gcctattgct ggacgccgac cagatcctcg cccgctggtt
     1141 gcacgacgcc aacgaagaac acaccatccg cgatttggtg caggagctag gccgcttaca
     1201 ggccggtgcc gcccaggcca atctgccgac catggcagag ttgagtggcc gcttggccct
     1261 tctgcacgaa ctggccctgg cggccctacc gcaagcgacg gcggcctatg tagaacacct
     1321 attgcccgcc cacgaattcc tcttggatat ggtggatgcg gtggccgcag gccaaaacct
     1381 gcccgacatc cccgccgcct tgcaagaggg cctcggcgag cttaatgaac agttgagcga
     1441 tatgctcgcc gcccagcaca ggggggcaga cgaagagctg gtgctggcaa gccttgagga
     1501 gctggaagcc gccgccgatg gcagcgacca tgaggatgag gcggatgccg ccatggccca
     1561 catacttgcc gaggaagagt ctgctgagca tgacgagacc gagcaggtac ctgtgctgga
     1621 tatagaagcg ctgcagctgg atcacgaaga attcgacctc cccaccctgg atgaattgga
     1681 agctgccgtt gcagctcaag aatccgaaac tcaagaatct gagcccctag aattcgagac
     1741 ccaatctcaa cccactgaga ttgaagacca ggcattcgac gccgaggtat ctgcagcctt
     1801 agccgcagag gaatccgtct tcgcagacga gcaggcgcaa gccgagccag cattgccaga
     1861 ggattgggcc gccgctgagt ttgatagcgc aagcttcgat catgaagccc tggcagctga
     1921 caccagcgag cctgagctgg ccgctacagc agaggaatta ttcaccttag agcccgatgc
     1981 tgatgaggcc gccgagctgg ttgctctgga ggccgaagag cacctggacc ctattctcgg
     2041 cgatatggat ctcgacctgg tcaccgccat cgacagccac ctcagcgccg acgacaccct
     2101 caccctgttg gatgccgacg ccgaggatat gctgctgccc agcgccgagg agaccttcgc
     2161 cgctgcggtg gatgacgacg acgtgatagt gccgcttctg gatgagttgg tggatctaaa
     2221 cgccgaagac gagccgctga tcagcgccct ggatgatttc gacccgattc cggtggtaac
     2281 cgacagcctg gatctggatg ccatagagga tggggaagcc ggcgaagaaa gcgaattcgg
     2341 cctggctgat gccgatgatt acgccgcaag cgagccctat gaggatgaaa tcgccctggg
     2401 tgattttgat ctgggtgagg aagacctcgc cgaactgctg gcgcaaaccc gcgagcctgc
     2461 cgaagacgcg cctgcaccgg tgcaggctgc cacagcgacg cccgccgccc cggttgccag
     2521 cgcctccttc ctgccggaag ccagccgcga taagcccgcc agcgagcggg tggaggatga
     2581 cttcgaccca gaaattctgg agattttcct ggaggaagcc gacgaattaa tggaggagtt
     2641 ggacgagtcc atccacgcct gggagtccga ctgggacagc agcgaagccc cggatgtgat
     2701 gaagcgcgcc ctgcatacac tcaaaggcgg cgcccgtctg gcctcgcaga tccaattggg
     2761 ggagctgacc cacaattacg aaagttattt gatcggcgtg ggcggcccca agggggatgt
     2821 gaatttcttc gccaccatcc tcgcctacca agaccagctg ctcaaatcca tccgcaagtc
     2881 ccgccagttg atggacggtg ttgccagcga agtggaagag agcgatgagg ccgtggacgc
     2941 gccgacgctg acggccaaaa tcgccgaaat cgcctccacc agcagcccgg tgccgtttgt
     3001 cagcagcaaa aacgtggtgg ccgtggcgcc ggaagacacc aaagaagtgg tggaagcctt
     3061 cgcaagcccg gtggttacca atctgccgga ctttgtggct cgctccaaaa gcggctccca
     3121 agaggtcatc aaggtctcct cagacctgct cgaagaactg gttaacctgg cgggggaaac
     3181 ctccatcagc cggggccgta tcgagcagca aatcaaaggc tttggcgccg ccattaacga
     3241 gatcgacggc accctgcgcc gcctccaaga gcagctgcgc cgcctagaca tagaaaccga
     3301 agcgcagatc ctcttccgcc aggagcaatt ggcggagaac gaaaacttcg acccgctgga
     3361 aatggaccgc tactcccagt tgcagcagct ctcccgctct ttaaccgagt ccgcctcgga
     3421 cctggtggac ctaaagcgca ccctgagcga gaaggtcaaa gacaccgaag cgattttgct
     3481 gcagcaatcg cgcatcaact cctccctgca ggaaggcctc atgcgctcgc gcatggtgcc
     3541 cttctcccgc ctggtgccgc gcctgcgccg catagtgcgg caggtggctg gcgagctggg
     3601 caaacacgtc agcttcgagc tggataacgt agaaggggaa atggatcgct cggtacttga
     3661 gcgcatggtg gcgcctttag agcacatgct gcgcaacgcc gtggaccacg gtattgagcc
     3721 ccccgctgag cgcctggcca agggcaaacc cgaatttggc cgtattttat tatccctggc
     3781 ccgcgagggc agcgacattc tgctgcgttt ggcagatgac gggcgcggta tcagcctgga
     3841 taaggtgcgc aaaaaagctg ttgagcgcgg cctgatgggg gagcactccc agttgagcga
     3901 ccacgaagtc atgcaattta ttttgcaggc gggctttagt accgccgaga gcgtgacgca
     3961 gatttccggc cgcggcgtcg gcatggatgt ggtggccgcc gagattaaac agctgggcgg
     4021 ctctatgacc attgagtccc gcagcggcgt gggttcgcaa tttaccgtgc gactgccgtt
     4081 taccgtatcg gtgaaccgcg ccctgatggt caccctcggc aaagacacct atgccattcc
     4141 cctcaacgcc attgaaggta ttgtgcgggt cagccccttt gagctggagc actactacca
     4201 gcagccggaa gcgcgttttg aatacgccga tgaaaactac caggtgcgct acctaggcgg
     4261 catgttgtac gaaggcacca cgccgaagct tgaaggccag ctgctgccgc tgccggtgat
     4321 tctggtgcgc tccgcccaac acaccatggc gttgcaggtg gacagcctgc aaggtagccg
     4381 ggaaattgtg gtgaagagcc tcggcaaaca gtttgccgcc gtacagggcc tctccggcgc
     4441 caccgtcatg ggcgacggca gcgtggtggt gattctggat gttcacgcat tggtgcgcaa
     4501 agccgtggcg tttgtggacg cactgcccct caaccacgag cgcaccaagg ccttaaacgc
     4561 cccgagggtg caaaaaaccg tcatggtggt ggacgactcg gtaaccgtgc gcaaggtgac
     4621 cacgcgcttt ctggagcggg aaggttatct ggtgatcacc gccaaagacg gcgtggacgc
     4681 cctgaagatt ctgcaggacc acactcccga tctgatgctg ctggatatcg aaatgccccg
     4741 tatggacggc tttgaagtgg cgaaaaacat tcgcaccacc gcccgttgga agcatatacc
     4801 catcgttatg atcacctccc gcaccggcga caaacaccgg gagcatgccc tgagcatcgg
     4861 cgtgaatgag tacctgggca aaccctacca ggaggatgtg ttggcgggca tcgtcagtcg
     4921 catcctcggt gacaaaggcc cctaagcggt gaagcttacg ggcttggcgg tgggggtatt
     4981 ggccgaatct aagccacagc tattggactt gcaccgcctg ctcgcgccct tgccccacac
     5041 gctagtggcc agccaagtgt tcagcggcga aaccctcggc agcctgcccc gggcggatgt
     5101 atggctggtg cagctggata tggacgaccc ggcggctcaa tctctgctgg atgccatgga
     5161 cgacgccaac ctgccggtga ttgtggccga tgaagacgag gcccccacgg atgcgacaga
     5221 ccccagccaa tggccggagg tgcgccgccg ggagcgggtg cgcaggctgc aaacaaaatt
     5281 agatttattg atcgaccaac agccccaaaa ggccaagcgc gcaaccgccg tgtgggtgct
     5341 ggctgcctcc accggtgggc cggaagcggt agggcaattt ttggaggccc tggaccagcc
     5401 gccggagcag ctggcgctga tctatgccca gcacataagc cccagcggcc ataaatccct
     5461 ggtgcagatg ctgcgccagc gcacgggcct taaggtgtgt ggcacccatc agaaccaggc
     5521 catcgaggcg ggctgcctgt atgtggtgtc ccccaatcag gccctggaca tagtcacccc
     5581 cggccgcata acccccaaac aacagccttg ggccgggcgc tttacgcctt ctatcaacca
     5641 ggtgatcgcc aaggtggcgc gggtgtatgg cgcccagggc ggcgcggtga tgtttaccgg
     5701 catgggggag gatggcgccc gcggctgcca attgttgcac cacttgggcg gccaggtgtg
     5761 gatccaaacc cccgccagca gcgctattga ttcaatgccg cgcagcgtgg agcgcctagg
     5821 ctgcacccgc tttgcgggca atccgcggga gctcgcccag gccctgcagc ggcgcttaaa
     5881 ccgcacccag gtatcggtgt gaacctagac tattttcacc ctcagcggcc ctaaaccccg
     5941 ctgaggcatt agccccaaga caggatctgt ttatgagtga gccctctgca tctgaacccg
     6001 gcaccatcac ctgcatgatg ctacccatcc aagcccgcac cctattggtt cccttggcaa
     6061 atattgccga aatggcggcg gtggcaccta tcttccc
//
LOCUS       DAOQGS010000221         6089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6452_length_6089_cov_3.887802, whole genome shotgun sequence.
ACCESSION   DAOQGS010000221 DAOQGS010000000
VERSION     DAOQGS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_6089_cov_3.887802"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..4501)
                     /locus_tag="PKZ52_09545"
     CDS             complement(302..4501)
                     /locus_tag="PKZ52_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG60059.1"
                     /translation="MRWHSSSVSALTTYAFNCAGRRWFACWLLCLAIAQLVWVQHAKA
                     APLLLSQYGREVWTSREGLPEGAVLSLLEDDQRQLWVGSECCLLRFDGETFTRLDNQP
                     SFGQHSFAQVLIRAEPKGMWAGFAGGLAFFDGAQFQWFAEAQGLTHPYVYSLASSAAT
                     LWIGTGGEGIWRLKAGQMARDSSFSQSDMPAMIYRLLSDSSGRLWAATESGLLTQNTE
                     GSWQKVATQGLPAQSISHLAEGRPGEVWVGTSAGLARWNGTAFEAYAPLLNLPITALY
                     ADRNHMLWVGSGDTLFRIGSEGVESLALNQGTILSLADDSFGDMWVGTSNGLLRLTPG
                     PIATFGKREGLLVDDLKSALPRRAGGVWLLDASGTLGYFANGSFAQVTQVGAIKGGAL
                     LGMTEDADGTLWIAGTELYRFAAGQLTTHEIPGEGLSLVERVGDELWLVQTAPNGTSS
                     LSRFRDGKLEPINLGQPVRHIQRIYQDRAGQVWITSSGSGVLRITGGEVQHFSQADGL
                     PSDTVYGITEDGDGRIWLATRGGIGLIERNRAARFNDIKGAPQNATVHIYLDAKDRLW
                     VSESGVGVHLLSRSALLKAVATQASEVPAELLTIRDGLRSLAFSWRRGGMAAVDGALY
                     LLGERGLSVIDTSLYQAPIFQGPVTINGLKLGEQTLSGNRLTLHNRSDVIEISYGAVN
                     LADASRLEFRTRLGDGRSPWSPPTSARSVRLSNLAPGSYRFEVAVRHQGQPWLADTAT
                     LAIEVPPYWYERTWVKSLAALWVVLLGLGYFRWRIRNAKQLEAYLRTTVHERTAELHQ
                     EISERKLAESKALDLAQNLEERVQERTVELEFAKMAVHKSEERFALAVQGAEDGIWDW
                     DLSQHTLYLSPRWKAILGYKDHEFPSTIDAWLSAVHPDDLDLVRDLLIFSDTHPNIRQ
                     EYRMLHRDGREIWVLCRGVVIRDNTYGLPLRAAGSQTDISQRKHTEDALRESLTRDPV
                     TELPNRALFLDRLDQALLKRRHQDTQVTVVLLDIDGFKALNEKYGHAAGDDILREVAR
                     RILSVLRQTDTLARLGNDEFALLLLEIEEEHQAQFALERIKNVLDAQVVIAQHSIKMS
                     YSMGIKHIQPGETHADEMLITAENALHQAKQQGGNRVQTVDKKTKGRRELEQELLHAI
                     HENEFTLYYQPLINLTTHSIVGAEALARWNHPKRGLLGPAHFISLLEACGLMDLFSDW
                     VLDTCCKQIALWREEFTDFSLRVSFNLPATHLNSPQLVDQIMAALQTHAIPASSLGIE
                     IVESSLIESNTVVLNNLERLRDIGLPVSIDDFGTGYSSMNYLKHFPVDCIKLDKSFCQ
                     GVPDNARDVAICESLLAIAEQLGLRAIVEGVETEKQMLFFKSKPQLIAQGYYFSKPVP
                     AWECAQMIREYNHIHLLKAAP"
     gene            4627..5448
                     /locus_tag="PKZ52_09550"
     CDS             4627..5448
                     /locus_tag="PKZ52_09550"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNG60060.1"
                     /translation="MSARLRGSIAYGPRVVLLALSCQPEHTPMPYEINGFGQPLGERL
                     PAWSPPPYPSGQVLKGRYCQLEPLQPHHTADLYRALGTSASDPGWTYLPYGPFASEAE
                     FTQWLSGQCEQSDPFFYALLNPAGEALGLASYLRIAPQAGSIEVGHLHFGAAMARTPM
                     ATEAMYLLMQQAFAWGYRRYEWKCNALNGPSRRAAQRLGFSFEGVFRQAMVNKGRNRD
                     TAWFAVIDRDWPALQAAFLTWLAEENFAADGSQRLRLSDLTSPLLVQRDTGFLGA"
     gene            5568..>6089
                     /locus_tag="PKZ52_09555"
     CDS             5568..>6089
                     /locus_tag="PKZ52_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HNG60061.1"
                     /translation="MNPLDIFEIPSTQPSTSCVIWLHGLGASNRDFVDALPYLKLPSQ
                     LAVKFIFPQAPSIPVTINQGYIMPAWYDILNIKIERTVDQKQLIASAEQIKSIIEQQI
                     NQGIPAKRIVLAGFSQGGAVAIQTALTCQHPLAGLAVLSSYLAIPEAFNQAGAKHKLP
                     VLIQHGNQDDVVPL"
BASE COUNT         1293 a         1769 c         1747 g         1280 t
ORIGIN      
        1 acctacgggt aaagattcac ctaaaaactc ttaacttaat gacatgggtt tgagttatgc
       61 aaagcccccc cctaaaagca ggccacaacc attcctcgcc cccacgccct gcgtggaaat
      121 gcataggttt aaattttggg agaaacatcc gccggagtac agtgaagcgg tatccattcc
      181 cacgcagagc atgggaacga gaaaacgaga aaacgagaaa acgagaaaac aaacagttat
      241 tttatagtgg tttaacacct gcatggcatc cagtgccact taacaatggg tgaagtttat
      301 cctagggcgc agcttttaat aaatgaatgt gattgtactc gcgaatcatc tgggcgcact
      361 cccaggccgg gacaggtttg gaaaaataat aaccctgggc aattaattgc ggtttggatt
      421 taaaaaataa catttgtttt tctgtctcta cgccttccac tatagcccgc aggcccagtt
      481 gttcggcgat ggcgagcagg gattcgcaaa tggctacgtc gcgggcgtta tccggcacgc
      541 cttggcagaa ggatttatcc aatttaatgc agtccaccgg gaagtgtttt aaataattca
      601 tactgctata gccggtgcca aaatcgtcga tggacaccgg caggccaata tcccgcaggc
      661 gctctaaatt gtttaatacc acggtgttgg attcaatcag gctgctctcc acaatttcaa
      721 tgcctaaact gctggcggga atggcgtggg tttgcagtgc cgccataatt tggtcaacca
      781 attgcgggct gtttaaatgg gtggcgggta aattaaagga aacccgcagg gaaaaatcgg
      841 taaattcttc gcgccatagg gcaatttgtt tgcagcaggt gtccagtacc caatcgctaa
      901 ataaatccat taagccacag gcttcaagca aactaataaa atgcgccggg cctaataaac
      961 ctcgcttggg atgattccag cgggctaagg cttcggcgcc cactatgctg tgggtggtta
     1021 aattaattaa gggttggtag tagagggtaa attcattttc gtgaatggcg tgcagtaatt
     1081 cctgttctaa ttcccggcgg cctttggttt ttttatccac cgtttgcacc cggttgccgc
     1141 cctgctgttt ggcttggtga agggcgtttt cggcggtgat gagcatttca tcggcgtggg
     1201 tttcgcccgg ctggatgtgt ttaatgccca tgctgtagga cattttaatg ctgtgttggg
     1261 cgattactac ctgcgcgtcc aacacgtttt taatgcgctc cagggcaaat tgcgcctggt
     1321 gttcttcttc aatttccagc aataacaggg caaattcatc attacccagg cgcgccaggg
     1381 tatcggtttg gcgcagcaca cttaagatgc ggcgggccac ttcgcgcagt atgtcatccc
     1441 ctgcggcgtg gccgtatttt tcgttgaggg ctttaaagcc gtcgatgtcc agcagcacta
     1501 cggtcacttg ggtgtcttgg tggcgccgct ttaagagggc ctggtccagg cgatccaaaa
     1561 acagggcgcg gttgggcagc tccgtgactg ggtcccgggt gaggctctcc cgcaaggcgt
     1621 cctcggtatg tttgcgctgg ctgatgtcgg tttgcgagcc tgccgctcgc aggggtaggc
     1681 cgtaggtgtt atcgcggatc actacgcccc ggcagagcac ccaaatttcc cgcccgtccc
     1741 tgtgcagcat gcggtattcc tggcggatat tggggtgggt gtcggagaag atcagcaggt
     1801 cgcgcaccag gtctaagtcg tccgggtgca cggcgctaag ccaggcgtcg atggtggagg
     1861 ggaattcgtg gtctttatag cccaaaatcg ccttccagcg gggggatagg tagagggtgt
     1921 gctggcttaa gtcccagtcc caaatgccgt cttcggcccc ctgcaccgcc agggcaaaac
     1981 gctcctcact tttgtgtacc gccatcttgg caaactccag ctccaccgtg cgctcttgta
     2041 cccgctcttc aaggttttgg gccaaatcga gggctttgga ttcggccagt ttgcgctcgc
     2101 tgatctcctg gtgcagttcg gcggtgcgct catgcaccgt ggtgcgcaaa taggcctcaa
     2161 gttgtttggc attgcggatg cgccagcgga aataccccag ccccagtaac accacccaga
     2221 gcgcggccag gcttttcacc caggtgcgct cgtaccaata gggcggcacc tcaatggcta
     2281 gggtggcagt atcggccagc caaggctgcc cctggtggcg cacggccact tcaaaccggt
     2341 agctgccggg ggccaggtta gagaggcgca ccgagcgggc gctggtggga gggctccagg
     2401 gggagcggcc atcgcccaga cgggtgcgaa attccaagcg gctggcatcg gccaggttca
     2461 cggcaccgta gctgatttcg atcacatcgc tgcggttgtg gagtgtcaga cggttgccgc
     2521 tgagagtctg ctcccccagc ttcaggccgt taatggtgac cggcccctga aatatcggcg
     2581 cttggtacaa ggaggtgtcg attaccgaca ggccccgctc accgagcaag taaagagcgc
     2641 cgtcaacggc tgccatgccg ccgcgccgcc aggaaaaggc caggctgcgc aggccatcgc
     2701 ggatggtgag caattccgcg ggcacctcgc tggcttgggt tgccaccgct ttgagcagag
     2761 ctgagcggga taacaggtgc acgccaacgc cagactcact cacccacagg cggtccttgg
     2821 catccaaata aatatgtacc gtggcgtttt gcggggcgcc tttaatatcg ttaaagcgcg
     2881 ccgcgcggtt gcgctcaatc aggccgatac cgccgcgggt ggcaagccaa atgcggccgt
     2941 cgccgtcttc agtaatgccg tataccgtgt cgctgggcag gccatcggcc tggctaaagt
     3001 gctgcacctc gcccccggta atacgcagca cgcccgagcc actggaggta atccacacct
     3061 ggcctgcgcg gtcttggtag atgcgctgaa tgtgccgcac gggctggcca aggttgatag
     3121 gctcgagttt gccgtcgcga aaacggctaa ggctgctcgt gccattgggg gccgtttgca
     3181 ccagccacag ctcatccccc acccgctcca cgagcgacag cccctcgccg ggaatctcat
     3241 gggtggtcag ctgcccggcg gcgaagcggt acagctcagt gcccgcaatc cagagtgtgc
     3301 catcggcatc ctcggtcatg cccaacaggg cgccgccctt aatcgcgccc acctgggtca
     3361 cctgtgcaaa actgccattg gcgaaatagc ccagggtgcc gctggcatcc agtagccaaa
     3421 cgccccccgc ccggcgcggc agcgcgcttt taaggtcatc caccagcagc ccctcgcgct
     3481 tgccaaaggt ggcgatgggg ccgggggtaa ggcgcagcag gccgttgctg gtgcccaccc
     3541 acatatcgcc gaagctgtca tcggccaggg acaaaatagt gccctggttg agggctaagg
     3601 actccacacc ctcgctgccg atgcgaaaca gggtatcgcc actgcccacc caaagcatgt
     3661 ggttgcggtc ggcgtagagg gcggtgatcg gcaagttcaa caggggggca taggcttcaa
     3721 aggcggtgcc gttccatctg gcaagcccag ctgatgtgcc cacccagact tcaccggggc
     3781 ggccctcggc caggtggctg atggattggg ccggaaggcc ctgggtggcc accttctgcc
     3841 agctgccctc ggtattttgg gttaacaacc cactctccgt ggcggcccac aggcggccag
     3901 aggaatcgct cagtaggcgg taaatcatgg cgggcatgtc gctttggctg aaggagctat
     3961 cgcgagccat ctgcccggcc tttaaacgcc agatgccctc gccgccggtg ccaatccaga
     4021 gggtggcagc gctgctggcc agggagtaca cataggggtg ggtgaggccc tgggcttctg
     4081 caaaccactg gaactgggcg ccatcaaaaa acgccaggcc accggcaaac cccgcccaca
     4141 tacccttagg ctcggccctt atcaacacct gggcaaaact gtgttggccg aaactcggct
     4201 ggttatccag gcgggtaaag gtctcgccgt caaagcgcag caggcagcat tcactgccca
     4261 cccaaagctg ccgctggtca tcttccaaca gcgacaacac cgccccctcc ggcaggccct
     4321 cccggctggt ccacacctcg cggccatatt ggctgagcaa caggggagcc gcctttgcat
     4381 gctgcaccca aaccagttgc gcaatggcca ggcacagcag ccagcaggca aaccagcggc
     4441 ggcctgcaca gttgaatgca taggtagtta aagcgctaac gctactggaa tgccatcgca
     4501 cgcgtctgtc caagccctca ggtgccaaca caattggcgc cagttttcaa agtaccccaa
     4561 acgagtttag acgagcccca gctaaacatc tgtcgatggc cgcaggttct aggcataccc
     4621 agggtgatga gtgcgcgatt gcgcggcagt atcgcttatg ggccaagggt ggtactcttg
     4681 gcactctctt gccagcctga acacacacct atgccctacg aaatcaacgg cttcggccag
     4741 cccctcggtg aacgcctgcc cgcctggagc ccacccccct acccgagcgg gcaagtgctc
     4801 aaaggccgct actgccagct tgaacccctg cagccccacc acaccgccga tctctaccgg
     4861 gcccttggta cctcggcgag cgaccctggc tggacctatc tgccctatgg cccgtttgcc
     4921 agtgaagcgg aatttaccca gtggctgagc ggccagtgtg agcagagcga tccgtttttt
     4981 tatgcgctgc tcaacccagc aggagaggcc ctgggactgg ccagctacct gcgcattgcg
     5041 ccccaggcgg gctccattga ggtggggcat ctgcattttg gcgccgccat ggcgcgcacg
     5101 cccatggcca ctgaagccat gtatttactg atgcagcagg cctttgcctg gggctatagg
     5161 cgctatgaat ggaaatgcaa tgccctcaac gggccttcgc ggcgagcggc ccagcggctg
     5221 gggttttcct ttgagggcgt cttccgccaa gccatggtga ataaaggccg caaccgcgac
     5281 accgcctggt ttgccgtcat cgaccgcgac tggcccgccc tgcaagcggc ctttttaacc
     5341 tggttggcgg aggagaattt tgccgccgac ggcagccagc gtctgcgctt aagcgacctg
     5401 acatcccccc tgttagtgca gcgggatacg ggatttctgg gggcttaagg ttcgctgtcg
     5461 ctggggtaaa aacaattcac cagtggcatt caacctaaac acaacaactg aggttgccgt
     5521 aaatttttta cgcgctaaaa acatcgaacc gaacaacagg tatcgccatg aacccattag
     5581 atatttttga aattcccagt acgcaaccca gtaccagctg cgtgatttgg ctgcacggcc
     5641 tgggggcgag taaccgagat tttgtggatg ccctgcccta cctaaaattg cccagccaat
     5701 tagcggtgaa atttattttc ccccaggcgc catcaattcc cgtcaccatt aatcagggtt
     5761 atatcatgcc cgcctggtac gatattctga atattaaaat tgagcgcacg gtggatcaaa
     5821 aacagctaat tgcctcggcg gaacaaatta aaagcattat tgagcagcaa attaaccagg
     5881 gtataccggc taagcggatt gtactggccg ggttttccca gggcggcgca gtggcgattc
     5941 aaaccgcatt aacctgccag catcccttgg cgggcttagc ggtgttatcc agttacttgg
     6001 cgataccaga ggcctttaat caggctggtg caaaacataa attgcccgta ttaattcaac
     6061 acggcaacca ggatgatgtg gtgcccctc
//
LOCUS       DAOQGS010000222         6085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6462_length_6085_cov_4.588723, whole genome shotgun sequence.
ACCESSION   DAOQGS010000222 DAOQGS010000000
VERSION     DAOQGS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_6085_cov_4.588723"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PKZ52_09560"
     CDS             complement(<1..178)
                     /locus_tag="PKZ52_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNG60062.1"
                     /translation="MDTAHYILQHTFGYSAFRGQQEDIIQALLQGEDALVVMPTGGGK
                     SLCYQIPALARPGVA"
     gene            433..4041
                     /locus_tag="PKZ52_09565"
     CDS             433..4041
                     /locus_tag="PKZ52_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNG60063.1"
                     /translation="MNTPLFILGIDVGSTTVKLVAIKGQSLDIAWSCYQRHETRQAEK
                     VLELLERVSEELPGLVLTQTPIFITGSGAGPIAPHINAKFIQEVNAVTMAVEHLHPDV
                     GSVIELGGQDAKIIMFKTQEESGEKRATTSMNDKCASGTGATIDKCFIKVGMPQEEVA
                     KLVFNPHKLHHVAAKCGVFAETDIVNLVKSGIPAAEIMCSLADAIVGQNLSVLTRGNT
                     LKPKVLLLGGPNTYLPFLQACWRYRIPQTWEERGFNYPRDVSIEELIFAPSNAEYYAA
                     FGAVLFGRHEETITPCSGLEPLAFFIKEGRKAQLGDQAGPALVKSNAEVEDFYRAYTL
                     PKFEPINLQDVLNKQGQAVIEGYIGLDGGSTSSKCVLVNDDGEILQKVYQLSKGNPLE
                     DMKQMFLQLKTWAEQQGCKLNVKGFGVTGYAGDVMESALKADANVVETVAHMKSAQQY
                     FPDVDVICDVGGQDIKVLFMQNKVIKNFRLSNSCSAGNGMLLQAMADQFGVPVTEYAD
                     RAFKADLSPKFSYGCAVFLDSDRVNFQKEGYNGEELLAGLALVLPKNIWQYVVQIPRM
                     AELGRVFVLQGGTQKNMAAVKAQVDYIKERVPNAEVYVHPHTGEAGALGAAFEAIRVV
                     KRQGRSRFIGLDEAINLRYTTRNDETTQCHFCANDCSRTFIDAHISETETARYISGFS
                     CEKGTVEDHDALKKLNKQRRERMVAYPNLVDYEATWCFKRLYKTEALPEAGSQVQDIE
                     VKKTFFGGVKHVPVARGFERSDAAAQTKREQIRIGMPKALNMWSTGPFWRAYFEALGV
                     KPRNVVFSDDTSEEMWQAGGKYGSIDPCYPAKVAQAHIHNLLFVHHEEKPLNLIFFPS
                     LTHIPTFVESVMDTACCPIVAGTPKVMGAAFTKEQDYFAARDITYLDPALSLNEPLLL
                     KNKMWQTFAEHLHITEDENNFAVDEAFKAWHYFDQHMQAKGRLILDAVAEQNRIALLM
                     IGRPYHNDPGLNHSVLEEYQAMGYPILSMRSIPKDKDYLAPYFAEDLKLGHITSPLDI
                     SDVWPENYSVNSVQKVWAAKFAARHPNVAVLDLSSFKCGHDAPTYGLIDRIIGANNSP
                     YSALHDIDANKPSGSIKIRVKTYGYTLKLNEEKLEDEAKLKKQAHLERVKQRMFALQQ
                     APEKIRNDPLIIAELEKCRQELAAAKAQYAANDQGFNVKPMVEVVTPQLIAAVEID"
     gene            4051..5835
                     /locus_tag="PKZ52_09570"
     CDS             4051..5835
                     /locus_tag="PKZ52_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60064.1"
                     /translation="MNAPVQDPAREPSAAEFLQQLDAQLIPAAEPTASQKHWFDEVPS
                     TFTAEQRAHTTILHAGLTMAHDLFIQSAFRGLGYKVHAMDVPDNDSLQLGREFGNRGQ
                     CNPTYFTVGNLVKQLKTMHEGGMSKEDIIKNYIFATAGSCGPCRFGTYVTEYRKALRD
                     AGFEGFRVILFQQSGGIKQATGQEMGLKIDAQFFLTMVRAILLGDVLNALMYRIRPYE
                     LEPGRTDAVINNAKQRIARAFETRSGLIKALWQTRKEIAAIPVDYLRVKPKVAIIGEF
                     WAMTTEGDGNYKMHRFLEQEGAEVDIQLVVNWGLYLLWGAQYDLAERAKLKGVDKGIN
                     KDAQSESGGSKFALEGINVAKKRWMLKGAEYFMRGVFYSYAKILGLKDYHLPDMNFIA
                     QISHAFYDNNLRGGEGHMEVGKLIHNVVDQKVNMTLSIKPFGCMPSSSVSDGVQSLIT
                     ELSPAAIFLPIETNGDGAVNVYSRVQMQLFKAKQMAQRQQEETQQQLGIDEATLKTWI
                     AAHPEVTRASHRSPHVGACTALDTLHYLATHQQKSWLARTSEVLINSWGKAARHKAHA
                     IAAVERAAHDFKNRKQSAVAAVKFVEAR"
     gene            5855..>6085
                     /locus_tag="PKZ52_09575"
     CDS             5855..>6085
                     /locus_tag="PKZ52_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNG60065.1"
                     /translation="MYSTLGCHLCEEAEAVLLPLLVEAQAAGVTIEVEVIDIAEEGLI
                     DAYGVRIPVLGCAQTERELDWPFDVGQVRELLL"
BASE COUNT         1661 a         1520 c         1493 g         1411 t
ORIGIN      
        1 ccgccacacc ggggcgggcc agcgcaggaa tttggtagca gagggatttg ccgccgccgg
       61 tgggcatcac caccaatgcg tcctcgccct gcagcagcgc ttggataata tcttcctgtt
      121 ggccgcgaaa ggcgctgtag ccaaaggtgt gctgcaagat gtagtgggct gtatccatgg
      181 tgcttaaatt ttaaacaagg gcgatgcaac ggccgcagat tgtacgggga taacgcaggt
      241 caagccaggc aataacaatc ccttggcaaa attaggggca ataggctgcc gggaaaaact
      301 tcagaaccgt gaacctgtgc cgcagggttt ttcacagccc gtctatttaa gcgtctaata
      361 acccacctta gcgcttggtt ttaataacaa tttcagctag tgttcagcaa cactttcacg
      421 ggcgcagcgc agatgaatac acctctattt attctgggaa ttgatgtagg ctccaccacc
      481 gtcaaattgg tggctattaa gggccagagt ttagacatcg cctggagttg ctaccaacgc
      541 catgaaaccc gccaggccga aaaggtattg gaattattgg agcgggtgag tgaggaatta
      601 cccggcctgg tgctcaccca aacacctata tttatcactg gctccggcgc aggccctatt
      661 gccccgcata tcaacgccaa atttattcaa gaagtaaacg ccgtaaccat ggcggtggaa
      721 catttacacc cggacgtggg cagcgtaatt gaattgggcg gccaggatgc caaaataatt
      781 atgtttaaaa cccaagagga aagtggcgag aaacgcgcga ccacctccat gaacgataaa
      841 tgtgcttccg gcaccggcgc cactattgat aagtgtttta tcaaagtggg tatgcctcaa
      901 gaggaagtgg ccaaactggt ttttaacccc cataaattgc accacgtagc cgccaaatgt
      961 ggtgtgtttg ccgaaaccga tatagttaat ttagtgaaaa gcggcatacc cgccgccgaa
     1021 ataatgtgtt cactcgccga cgccattgtg gggcaaaatt tatccgtatt aacccgcggc
     1081 aataccctaa aacccaaggt tttattattg ggtggtccca atacctattt gcctttttta
     1141 caagcctgct ggcgctatcg catcccgcaa acctgggagg agcgcggctt taattatccg
     1201 cgcgatgttt caatcgaaga attaattttt gcccccagca atgccgaata ttacgccgcc
     1261 tttggtgcgg tattattcgg ccgccatgaa gaaactatca ccccttgcag cggccttgag
     1321 ccgctggctt tttttattaa agaaggccgc aaagcgcaat tgggcgatca agcgggcccc
     1381 gccctggtta aatccaatgc cgaggtagaa gatttttacc gcgcttatac tctgcctaaa
     1441 ttcgaaccga tcaatttgca ggatgtatta aacaagcagg gccaggcggt tattgagggt
     1501 tacattggtt tggatggcgg ctccacctcc agcaaatgtg tgctggtaaa tgacgatggg
     1561 gaaattctgc aaaaggttta tcaactctct aagggtaacc ccttagaaga tatgaaacaa
     1621 atgtttttgc aattaaaaac ctgggctgaa caacagggct gcaaactcaa tgtaaaaggc
     1681 tttggtgtga ctggttatgc tggggatgta atggagtccg ctttaaaagc ggatgccaat
     1741 gtggtggaaa ccgtcgccca catgaaaagc gcccagcaat acttccctga tgtagacgta
     1801 atttgtgacg tgggcgggca ggatattaaa gtcctgttta tgcagaacaa agtgattaaa
     1861 aacttccgcc tcagcaattc ctgtagcgca ggcaatggca tgttattaca agccatggcc
     1921 gatcaatttg gcgtgccggt caccgaatac gccgaccgcg cttttaaagc ggatttatcg
     1981 cccaaattca gttatggctg cgccgtattt ttagattccg accgagtgaa tttccaaaaa
     2041 gaaggctaca acggcgagga gttattagca ggccttgccc tagtgctgcc gaaaaatatt
     2101 tggcaatacg tagtgcaaat cccccgtatg gcggaattag gccgcgtatt tgtactgcag
     2161 ggcggcacgc aaaaaaatat ggcggcggta aaagctcagg tggattacat caaagagcgg
     2221 gtgcccaatg ccgaagtcta cgtgcacccg cacacaggtg aagcaggtgc cttaggcgcc
     2281 gcctttgaag ccatccgcgt agtaaagcgc cagggccgca gccgctttat cggtttggat
     2341 gaagccatta atttgcgcta caccacccgc aatgatgaaa ccacccagtg ccatttttgc
     2401 gcgaacgatt gctcccgtac ctttattgat gcccatatta gcgagacaga aaccgcccgt
     2461 tatatctctg gttttagctg tgaaaaaggc acagtggaag accacgatgc attaaaaaaa
     2521 ctcaacaaac agcgccgcga gcgcatggtg gcctacccta atttagtgga ttacgaagcc
     2581 acctggtgtt ttaagcgcct atataaaact gaagcactgc cggaagcagg cagccaggta
     2641 caggatatag aagttaaaaa aacctttttc ggtggcgtaa aacacgtgcc cgtagcacgc
     2701 gggtttgaaa gatcagatgc ggcagcacaa accaagcgcg agcaaattcg catcggtatg
     2761 ccaaaggcct taaatatgtg gagcacaggc cccttttggc gcgcctattt tgaagccttg
     2821 ggcgttaaac cgcgcaatgt tgtgttctct gacgatacca gtgaagaaat gtggcaggca
     2881 ggtggtaaat acggctccat cgacccctgt tacccagcaa aagtggcgca agcccatatc
     2941 cacaatttat tgtttgttca ccatgaagaa aaacccctta atttaatatt tttcccctcc
     3001 cttacccata tacctacttt tgtagaaagt gtaatggaca cagcctgttg ccccatagtg
     3061 gcaggcacac ctaaggtgat gggggcggct tttaccaaag agcaggatta tttcgccgcg
     3121 cgggatatta cctacctaga cccagccctc agcctcaacg agcctttatt attaaaaaat
     3181 aaaatgtggc aaacctttgc cgagcattta catattactg aggatgaaaa taacttcgcg
     3241 gtggatgaag cctttaaggc ttggcattat tttgaccagc atatgcaggc caaaggccgt
     3301 ttaatattag atgccgtagc cgagcaaaac cgcatcgcgc tattaatgat tggtcgcccc
     3361 taccacaacg acccaggcct aaaccacagc gtattggaag aataccaagc catgggctac
     3421 cccatattat ccatgcgctc catccctaag gataaagatt atttagcgcc gtattttgcg
     3481 gaagacttaa aactcggcca tatcacatcg cccctggata ttagcgatgt gtggccggaa
     3541 aactacagcg taaactcggt gcaaaaagtc tgggcagcta aatttgctgc ccgccacccc
     3601 aatgtggcag tgctggattt atccagtttt aaatgcggcc acgatgcgcc cacctatgga
     3661 ttgatcgacc gcattatcgg cgcgaataat tccccttatt ccgccctgca cgatatagac
     3721 gccaacaaac cctccggctc catcaaaatt cgcgttaaaa cctatggcta cactttaaaa
     3781 ctgaatgaag aaaaactcga agacgaagcc aaattaaaaa aacaggccca tttagagcgc
     3841 gtcaaacagc gcatgtttgc cctgcaacaa gcgccggaaa aaatacgcaa tgatccatta
     3901 attatcgccg aactggaaaa atgccgtcag gagcttgccg cagctaaagc ccaatacgcc
     3961 gccaacgatc aagggtttaa tgttaaaccc atggttgaag tggtaacccc tcaattaatt
     4021 gccgccgttg aaattgacta ggagcttatt atgaacgcac ccgtacaaga tcctgcccgc
     4081 gaacccagcg ccgctgaatt cttacaacaa ttagacgccc aattaatacc ggcggcagag
     4141 cctactgcgt cacaaaagca ttggtttgac gaggtgccca gcacctttac cgccgagcag
     4201 cgcgcccaca ccaccatttt gcacgcaggg cttaccatgg cccacgattt atttattcaa
     4261 tccgcctttc gcggtttggg ttataaagta catgccatgg atgtgccgga taacgactcc
     4321 ctacaattag gccgggaatt tggcaaccgc ggccagtgca accccacgta ttttactgta
     4381 gggaatttgg taaaacagtt aaaaaccatg cacgaaggcg gcatgagcaa agaggacata
     4441 attaaaaatt atatttttgc caccgcaggt tcctgcggcc cctgccgttt tggcacctat
     4501 gtaactgaat accgcaaagc gctgcgtgac gccggttttg aaggctttag agtgatttta
     4561 ttccaacaat ccggcggtat taaacaagcc accggccagg agatgggcct aaaaattgat
     4621 gcgcaatttt tcttaaccat ggtgcgggct attttattag gcgatgtatt aaatgccctt
     4681 atgtaccgca tccgccccta tgaattagaa cccggccgca cggatgccgt tattaataat
     4741 gccaaacaac gcatcgcccg cgcctttgaa acccgcagcg gcttaattaa agccctctgg
     4801 caaacccgca aagaaatcgc cgccattccg gtggattacc tgcgggtaaa acccaaggtg
     4861 gctattattg gtgagttctg ggccatgacc accgaaggtg atggcaatta taaaatgcac
     4921 cgctttttag agcaggaagg cgccgaggtt gatattcaat tagtggtgaa ctggggcctg
     4981 tatttactct ggggcgccca atacgattta gccgagcgcg ccaaattaaa gggtgtcgat
     5041 aaaggcatca ataaagatgc ccagagcgaa agtggcggca gcaagtttgc gctggaaggc
     5101 ataaacgtgg ccaaaaagcg ctggatgctc aaaggtgccg aatattttat gcgcggcgta
     5161 ttttatagct acgcaaaaat tttaggcctg aaggattacc acctgccgga tatgaatttt
     5221 atcgcgcaaa ttagccacgc cttttacgat aataacctgc gcggcggcga aggccatatg
     5281 gaagtaggca aattaatcca caatgtggtg gatcaaaaag taaacatgac cttgagcatt
     5341 aaacccttcg gctgtatgcc ctccagcagt gtttccgacg gtgtgcaatc tttaattaca
     5401 gagttatccc ccgccgccat atttttaccc atagaaacta atggcgacgg ggccgtgaac
     5461 gtatacagcc gcgtacaaat gcaattgttt aaagccaaac aaatggccca gcgccaacag
     5521 gaagaaaccc agcaacaatt aggcattgat gaggccactt taaaaacctg gattgccgct
     5581 cacccagaag tgacccgcgc ctcccaccgc agcccccacg tgggcgcctg caccgcactg
     5641 gatacactgc attatctagc cacccaccaa caaaaatcct ggcttgcccg cacctctgaa
     5701 gtgttaatta acagctgggg caaagccgcc cgccacaaag cccacgccat agcagcggta
     5761 gagcgcgccg cccacgactt taaaaaccgt aaacaaagcg cggtagccgc cgttaaattt
     5821 gttgaggcac gataatttgc agcgccggtt ttttttgtac tccaccctgg ggtgccattt
     5881 gtgtgaggag gcggaggctg tgttgcttcc gcttttggtt gaggcccagg cggcaggcgt
     5941 aacaattgaa gttgaagtga ttgatatagc cgaagagggg ttaattgatg cttatggggt
     6001 gcggattccc gtattgggct gtgcgcaaac cgagcgggag ttggattggc cgtttgatgt
     6061 ggggcaggta agggagttgc tgttg
//
LOCUS       DAOQGS010000223         6082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6472_length_6082_cov_3.938112, whole genome shotgun sequence.
ACCESSION   DAOQGS010000223 DAOQGS010000000
VERSION     DAOQGS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6472_length_6082_cov_3.938112"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKZ52_09580"
     CDS             complement(<1..261)
                     /locus_tag="PKZ52_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNG60066.1"
                     /translation="MSFFDSIKKMGKDASAKITSEVAKFKNKDFLNAVVAGCALVAAA
                     DGSISSAEKQKMLGFINSSDELKVFGADEVIAAFNNVVSKFDF"
     gene            complement(885..1343)
                     /locus_tag="PKZ52_09585"
     CDS             complement(885..1343)
                     /locus_tag="PKZ52_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60067.1"
                     /translation="MTADKSNLNEGVIELACIWVEQQQEYIGNFGAKLTPHQTALAVQ
                     VGVRQPECIRILTVERIPSPKDESLSKLAEKLNFLGPGTLGITLGYCIYVRDGYVDDR
                     LLSHEFRHVYQFEESGSIQSFVGEYIQQLIQYGYELSPFEIDAREHEVSN"
     gene            complement(1684..2841)
                     /locus_tag="PKZ52_09590"
     CDS             complement(1684..2841)
                     /locus_tag="PKZ52_09590"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNG60068.1"
                     /translation="MSHIESNKQLIDLVFERSSLIRDRLSFPSINDRFCEGSRAVLPW
                     INGYLNARDDYGYDVIKAKEYLSSIGQNSSDIANRFRGGMLGLALGDALGTTLEFSQR
                     DTLPELTEIVGGGPFNLKPGEWTDDTSMAICLAHSLIRKEGFVAQDVMDLFYLWWKRG
                     IFSVNNKCFDIGDTVRDALIRYENTQEPFAGNNSPTAAGNGSLMRLLPIVLFNFSNID
                     QCVEACASSSKLTHQATEAVDACRYFGALITAAIRGEPKDTITGSFYEPVPGFWQRRP
                     MTSSIVQLAANIHSRTRNEIKSTGYVIDTLEAALWAFHNSNTFENGALLAANLGGDSD
                     TVAAVYGQLAGAYYGEMRINTNWIRTLSKYHIFYHYADQLLRFGVCDYRPR"
     gene            complement(2841..>6082)
                     /locus_tag="PKZ52_09595"
     CDS             complement(2841..>6082)
                     /locus_tag="PKZ52_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNG60069.1"
                     /translation="GRGISFERNLQGRINKVYLRRLNTPEQKLLAEYDYSETGDLIAQ
                     RNVLGVGERYEYKNHLFTRRILATGFSYYYQWDGEDNTARCLRNWGDDGIYNYRFSWQ
                     PQFNQSSATDSNGYTNHYTYNEFGQIIKKIDKEGGVHSWDYRDGLCVRYTDPEGNCTY
                     YNYNEARDLISVTDPLNHSNSIEYTDGLISRSQDKNGAQWSYQHNTIGQLIAVTDPTG
                     QTTHLTYNAQGLLVEERQGNSRVRRYEWSDEAELIRVQHAQGHSQSFKYNGFGQLIQI
                     DVWARSRAHIGTTRYQYNDAGQLTQIIYDNGEKANYSYNANGLVHIIKDRQGRITRFD
                     YDGLSQVVRKTDPEGFTLEYQYDKERNLTALINQNGERYAFAYDGNERLISETGFDGR
                     QQTYQYNKAGHLISSTDGDNIVVNYRRDALGRMLAKSYRLANQDTAMEISRYRYNAAG
                     QLVETYNQNQYLEFSYDTFGRLIREEQTLIDEQNRLIKNSRKVVEFARNQQGALTELR
                     LPGDQLVNYAYDQFEQLESVGFNEKEITRIERDELGREISRQQGQVLSAFEYDPMGRL
                     IKQRAQHKANQTDIIDRRYEYDNAGNLSQVSDGAWQIKYVYDQLNRLKETQGDLAETF
                     VFDPASNLLGPDKNAQIKGNRLLMQADRKFTYDARGNLIHEQRGKGGVIQTAYRYNLA
                     NQLIEVNKNNQRIQFAYDPLGRRIYKQDDFGRTDFIWAGDQLAQEIRGGNVKTYIFEP
                     ESFKPLAVAQNDAVYHYHLDHLGTPKELTNEQGKIVWKARCKTYGSLALKEVDEVENN
                     LRFQGQYYDLETGLHYNRHRYYDPSIGQFISQDPIGLLGGVNNYQYAPNPTGWVDPLG
                     LSCKEIPENYDFLSGKFIDPIDAAQLNHNLGVDIEELLKSESNNFFLKDKNLIKNAKA
                     NYPNSPLSSHIEYHEYLAIMAYTDDFYKGINSALRSGTPGKWEGLASSANSGLNKLGQ
                     RPEFAAEGFMTRGMTLDNDMIHKLFPDGGEFSDKAFISSSHDGTKSFPGNVKLTMETI
                     TPQAKIGGISVYGDTEAEVLFKTDTKFDVVSKTQMPDGSWQIHIKEKAK"
BASE COUNT         1588 a         1395 c         1291 g         1808 t
ORIGIN      
        1 aaagtcaaat ttgcttacta cattattaaa cgccgcaatc acttcatcgg caccgaacac
       61 ttttaattca tcggagctat tgataaaacc tagcattttt tgcttttcag cagacgaaat
      121 agaaccatca gccgctgcca ccaaagcgca ccctgccacc acagcattta aaaagtcctt
      181 atttttaaat tttgccacct cgctggttat cttagcgcta gcatctttac ccattttttt
      241 aattgaatcg aaaaagctca tagtatttac tccagttagt taaaataaaa attgcatgtt
      301 tggccgctat ggagatagaa caagccccgc cgacacacct agctgcacac cggcaaagtc
      361 cccgtctgca aggcttagtg cagctgacat gaatctaggc aagcggcggc attgataaca
      421 gatgttatgg cggatagcaa tccaagtgct gttgcttgac cgtgaaccca ctagccctaa
      481 tcccgcccct aaccggcctg cgcttatggg gcaggatgac atcccccatt gcagaaggtt
      541 caaaatgccc acaccaggca aaggatctaa atagccaggt gcgccttaac ggaatgagga
      601 tattactgga tggctcaata aacaggccat tgccctatta attcacatga aatttcaagt
      661 gtgacacagc aaattcctta gcccagaaaa tgattcagca gcttaattgc agtttcgagc
      721 cctgcgatcg cggagtctac acgtttatat ttgcaaacca ataaatgagt ggggacatat
      781 ttaacttatg aaaaatatca ccaaagtcaa aataccacta acgaagccca actatttgga
      841 gtgagaaaaa gtttattaaa ttgccaatta actcctggca cccattagtt gctaacctca
      901 tgctcacggg catctatttc aaatggtgac aactcgtagc catactggat aagctgctgt
      961 atgtattcac caacaaagga ctggatagag ccggattcct cgaattgata cacatgccgg
     1021 aattcgtggg agagcagcct gtcgtccaca taaccatccc gcacatagat gcaatagcct
     1081 agagttatgc ctagtgtacc gggcccgaga aaattcagct tctcggcaag tttactcaaa
     1141 ctctcgtcct ttggagaggg aattctctcc accgtcaaaa tacgaatgca ttcgggctgc
     1201 ctgacgccaa cctgaacggc cagtgcggtt tgatgggggg ttaactttgc tccaaagttg
     1261 ccaatatatt cctgttgttg ctccacccat atacatgcga gctctatcac cccttcattt
     1321 aaattagatt tatcagcagt cataaataag ctcacagata tttgtttgac taaattgggg
     1381 ttgttacact ttttcgccag taatgtatct tttctgcagc atccgttggg gtatgcattc
     1441 accatacatt gattaaaaat caatccttga atcatagacg tagattaatc agtataggcc
     1501 gacaattatc cactttaacc catagccaag ccaactgcat taatggatct gcaatgctgt
     1561 taaagttttt aacacctcat taaagtcaca gccgccacaa ttctctttgg ctatgaatag
     1621 ggaaaaaata catatcacta aagatcggag aatattaata ttgagtgtta atttaggtga
     1681 gggtcagcga ggacgataat cgcacacacc aaatcgtaat aattggtctg cataatgata
     1741 gaaaatatgg tatttgctca gcgttcttat ccaattggtg ttgattcgca tttcgccgta
     1801 ataagcacca gctaattgtc cataaactgc tgcaacagtg tccgaatctc cgcccagatt
     1861 cgcggccagt aacgcgccgt tttcaaaggt atttgaatta tgaaacgccc ataaggcagc
     1921 ttctaatgtg tcaattacgt accccgttga cttaatttca ttccgagttc gggagtgaat
     1981 attagctgct aattgtacaa ttgacgatgt catagggcgc ctttgccaaa aacctggcac
     2041 tggctcataa aatgatcctg ttattgtatc ttttggttct ccccttatcg ccgcagtgat
     2101 taaggctcca aagtatcgac atgcatcaac agcctctgtg gcttggtgtg taagcttaga
     2161 cgacgatgca cacgcctcga cacattgatc tatgtttgaa aaattgaaga gtacgattgg
     2221 taatagacgc atgagtgatc catttcctgc agctgtaggc gagttattcc cggcaaatgg
     2281 ctcttgtgta ttctcatagc gtatcaatgc atctcgcacc gtatcgccaa tatcgaagca
     2341 tttattattt acactaaaaa tgcctctttt ccaccataag taaaatagat ccattacgtc
     2401 ttgggcgaca aatccctcct ttcgtatcag gctatgagcc aagcaaattg ccatgcttgt
     2461 atcatctgtc cactcgcctg gctttaaatt aaaaggcccg ccacctacga tttcagttaa
     2521 ttcaggcaag gtatcccgtt gtgaaaattc caaggtggtg cctagggcgt cgcctaacgc
     2581 gagtcccaac atgcctcctc taaaacgatt tgcaatgtct gatgaatttt gacctatgga
     2641 gctcagatac tctttcgcct ttattacgtc gtagccataa tcatctcgtg cgtttaaata
     2701 tccattaatc catggaagaa cagctcggga gccttcacaa aagcgatcat ttatgcttgg
     2761 aaatgagagc ctgtctcgaa ttaacgaaga tcgctcgaaa acaaggtcaa tgagttgttt
     2821 atttgattct atatgagaca ttatttggcc ttttctttaa tgtgaatttg ccacgagcca
     2881 tctggcattt gcgttttgct aacgacgtca aattttgtat ctgttttaaa tagaacttca
     2941 gcttcagtat cgccatacac tgaaattccg ccaatctttg cctgcggggt tatggtttcc
     3001 atggtcaatt tcacgttacc tggaaaggat ttggtgccat cgtgagatga gctaataaat
     3061 gctttgtcag agaattcgcc accatccgga aataatttat gaatcatgtc attatctaat
     3121 gtcatgcctc gagtcatgaa accttctgca gcgaactcag gacgttggcc caacttgttc
     3181 aatccagaat tggcagacga cgctaaccct tcccactttc caggagtgcc tgatcttaac
     3241 gcagagttaa ttcctttgta aaaatcatca gtgtaagcca tgatggcaag atactcatga
     3301 tattcaatat gggagcttaa tgggctattt ggataatttg ctttggcatt tttaatcaaa
     3361 tttttatctt ttagaaagaa gttattagat tccgatttaa gtaattcttc aatgtcaacg
     3421 ccgaggttgt gattcaactg agcggcatca atcggatcga taaacttgcc actcaaaaag
     3481 tcataatttt ctggaatctc cttgcacgaa agccccaatg gatccaccca ccctgtcgga
     3541 ttaggtgcat attggtaatt atttacccca cccaataacc caatcgggtc ttggcttata
     3601 aactgcccaa tacttgggtc gtaatagcgg tggcggttgt agtgcaggcc cgtctcaagg
     3661 tcataatact ggccttgaaa gcgtaaatta ttctcaacct catccacttc tttgagagct
     3721 aggctgccgt aagtcttaca gcgggccttc cagactattt taccttgctc attggttaat
     3781 tctttgggtg tgcccagatg atccaggtgg tagtgataaa cggcgtcgtt ttgtgcaacg
     3841 gccagtggtt taaagctttc cggttcgaaa atataggtct taacattgcc gccacgaatt
     3901 tcttgcgcca attggtcacc cgcccaaata aaatccgtgc ggccaaaatc gtcttgtttg
     3961 tatatgcggc ggcctaatgg gtcgtaggcg aactgaatac gctgattgtt tttattaact
     4021 tctattaact gattggcgag gttatagcgg taggctgttt gaatcacgcc tcctttgccg
     4081 cgctgttcgt ggattaaatt gccgcgggca tcataagtaa acttgcgatc cgcttgcatc
     4141 aataagcggt tgcctttgat ctgcgcgttt ttatctgggc cgagtaagtt gctagctggg
     4201 tcaaatacga aggtttcagc caaatcacct tgagtttctt taaggcgatt gagctggtca
     4261 tatacgtatt ttatttgcca agcgccatcg gatacttggc ttaggttgcc agcattatcg
     4321 tattcataac gccgatcgat tatgtcggtt tgattggctt tatgctgggc gcgttgtttg
     4381 attaaacggc ccatagggtc gtactcaaat gcactgagca cctggccctg ttggcggctt
     4441 atctcgcggc caagctcgtc gcgctcaatg cgggtaattt ccttttcgtt aaagccaacc
     4501 gattccaatt gctcgaattg atcgtacgca taattaacta actgatcgcc tggcaggcgc
     4561 agttcagtca aggcaccttg ttggttgcga gcgaattcaa ccacctttct actatttttg
     4621 atcagcctat tttgctcatc aattagggtt tgttcttcgc gaattaaccg cccaaatgta
     4681 tcgtagctaa attcgagata ttggttttgg ttgtaggttt caaccagctg gccagcagca
     4741 ttataacgat agcggcttat ttccattgcc gtgtcttgat tggctaaacg gtaggactta
     4801 gccagcatac ggcccagtgc atcacggcga taattaacca ctatattgtc gccgtcagta
     4861 ctgcttatta ggtggccagc tttgttgtat tgataggttt gctgccggcc atcaaaaccg
     4921 gtttcactaa ttaggcgctc attgccgtca taggcaaatg catagcgctc accattttgg
     4981 ttaataagag ctgttaaatt gcgctctttg tcgtactggt attcaagagt aaaaccttca
     5041 ggatcagttt tgcgaacaac ttggcttagt ccgtcgtaat caaaacgggt gatgcgccct
     5101 tggcggtctt taataatgtg aaccaatcca tttgcgttgt aggaataatt cgctttttca
     5161 ccattgtcat agattatttg cgtcagttgg cccgcatcat tgtattggta gcgggttgtg
     5221 cctatgtgtg ctctgctgcg agcccaaaca tctatttgaa taagctggcc gaagccattg
     5281 tatttaaaac tttggctgtg cccttgtgca tgttgtacgc gaattaattc ggcttcgtca
     5341 ctccattcat agcgccgtac acggctatta ccctggcgct cttcaactaa tagaccttga
     5401 gcgttatacg taagatgagt agtttggcct gtgggatcgg taacggctat cagctggcca
     5461 attgtattgt gctggtaact ccactgcgcg ccgtttttat cctggctgcg gctgattaat
     5521 ccgtctgtat attctattga gttgctgtga ttgagtgggt cggtgacgga tattaagtcg
     5581 cgcgcctcat tataattgta ataggtgcag ttaccttcag ggtctgtata acgaacacat
     5641 aaaccatcgc gataatccca gctgtgaaca ccgccttcct tatcaatttt tttgataatt
     5701 tggccaaatt cattataggt gtaatggttt gtatagccgt tgctatcggt ggcgctactt
     5761 tggttgaatt gcggctgcca actaaaacgg taattataaa tgccgtcatc gccccagttg
     5821 cgcaggcagc gcgctgtgtt gtcctcgcca tcccactggt aataataact aaatcccgtg
     5881 gcgagtatgc gacgggtaaa caggtggttc ttatattcgt agcgctcgcc gacacctaaa
     5941 acattgcgct gggcgattaa atcgccggtt tcgctgtagt cgtattcagc taacaacttt
     6001 tgctcaggtg tatttagcct tcttaaatag accttattga tgcgcccttg caaattgcgt
     6061 tcaaacgata tgcctcgacc cc
//
LOCUS       DAOQGS010000224         6069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6497_length_6069_cov_4.885268, whole genome shotgun sequence.
ACCESSION   DAOQGS010000224 DAOQGS010000000
VERSION     DAOQGS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_6069_cov_4.885268"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKZ52_09600"
     CDS             complement(<1..432)
                     /locus_tag="PKZ52_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60070.1"
                     /translation="MRLPILTLSLIVGLTACGSGKGSSKNNGGQNPPNPSSSSSSSSS
                     SSSSSSSTGSANTSSSGGTTSSSSSSGGTNPKPDPSSSSSSSGNTGSNSSTTSSSGGT
                     GSSSSSTSSSSGGSETGSSSSSSNGGGSSTSSTSSSSGGSET"
     gene            1491..3116
                     /locus_tag="PKZ52_09605"
     CDS             1491..3116
                     /locus_tag="PKZ52_09605"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNG60071.1"
                     /translation="MPHLLTGYALLANVKYSKGTAFSLEERDAYGLRGLLPPRVCNMS
                     VQVQRALANLRRKTCDIDRYVFMQQLLTRSQTLFYRVLIDHIEELMPIVYTPTVGQAC
                     REFAHIYRQAQGFYISLADRGLVRSMLNNWPEKDIRMIVVTDGERILGLGDLGANGMG
                     IPIGKLALYCACAGVRPEQCLPVMIDVGTDNVPLREDPFYLGTPCPRVRGEAYLALVD
                     EFVQAVQDAYPKALIQFEDFLTPNAYMLLHRYRREVLCFNDDIQGTAAVALAGIYGAC
                     TLANLAFNQLRIMFLGAGSAATGIADLLVQALVEQGLTQQEARGRLWFVDVDGLVVSS
                     RPYLAEHNRPYAQDYPPMDFISAIDAIRPQVLIGATGAANAFTPDVIGRMAQYNVRPI
                     IFALSNPTANAECTASQAYEWSQGRAIFASGSPFPPVTYLGQFFRPAQGNNAYIFPGV
                     GLGAMLVEARLVSDEMFLRAARVLADCLSPMDLAEGALYPPLNKIRDVSLAIACAVAE
                     VAYQEGVARLPHPGDLKTFIAERMYDPNYEELK"
     gene            complement(3224..4195)
                     /gene="cmoB"
                     /locus_tag="PKZ52_09610"
     CDS             complement(3224..4195)
                     /gene="cmoB"
                     /locus_tag="PKZ52_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HNG60072.1"
                     /translation="MIDYRAFLTRISDGPLAPWAKILPEQIAQGLDIKRHADLNRWLT
                     AYTALPELTTDYRHALNAVEIGRGNEIAPAKHAELLSQLKNLMPWRKGPFNLFGITIN
                     TEWRSDWKWHRVLPHISDLTGRRVLDVGCGSGYHAWRMYGAGASEVIGIDPSPRFVVQ
                     FYMLKKYWPQAPVDVLPVTLDSMPANLHAFDSCFSMGVFYHRRSPMDHLRELRDVLRP
                     GGELILETLIIPEKHGQLLVPSERYAQMGNVWFIPSVPTLLNWLAKIGFKNARCVDIN
                     QTRCDEQRTTDWMHFNSLQDFLHPDDPNLTIEGYPAPRRAIFIAEKP"
     gene            complement(4237..5001)
                     /gene="cmoA"
                     /locus_tag="PKZ52_09615"
     CDS             complement(4237..5001)
                     /gene="cmoA"
                     /locus_tag="PKZ52_09615"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HNG60073.1"
                     /translation="MIAAHPARRLAMHSKTDTLFANPLTHLGDFCFDQTVVDVFPDMI
                     NRSVPGYATILRMIGQIAEGYARPNTYCYDLGCSLGAATLAMRHRINTSGTQIVAVDN
                     SGAMVEKARQVIAADAGEVPVSLHHANLQDIPIHNASVVVLNFTLQFIPVAERLNILQ
                     RIATGLIPGGVLILSEKLAFEDAEHQELMTQLHHNFKRANGYSDLEIAQKRTALEHVL
                     IPETYATHKQRLNQAGFRSTDLWFQCFNFASMLAFK"
     gene            5122..>6069
                     /locus_tag="PKZ52_09620"
     CDS             5122..>6069
                     /locus_tag="PKZ52_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60074.1"
                     /translation="MGDIDYGSMRCLVVDDFGNFRTTLAAMLSKMGWVQVDQASNVTG
                     LIAACEKRQYDLILTDYDLGPGRNGQRGLEELRHRKLISKRTLFVLVSGNASKEAVLA
                     TYDNEPDDYLAKPVTAKVLQQRLDRLFAQRQVLLPVYKALDNGDAARAVFLLTEMSIA
                     NNRHSIAAQKALGRLFIEQGELDKAERVFSLALQTRPLDWARLGLARVKALRGEKAIA
                     GEWMEQIISDSPLFLPAYDALADTCADAGQRQVEQATVQKAVNISPMSILRQKRLAEV
                     AEANGDWLTAMRAWRRAIKLGEESIHGEARDGIKFARAVA"
BASE COUNT         1338 a         1442 c         1750 g         1539 t
ORIGIN      
        1 ggtttcgctg ccgccggagc tgctggaagt agaagaagtg cttgagccgc cgccgtttga
       61 tgagctgcta gagctgccgg tctcactgcc gcctgagctg ctagaagtag atgagctgct
      121 agaccctgtg ccaccgctac ttgaggtagt ggagctgttt gagcctgtgt tgcctgagga
      181 tgagctgcta ctggagggat cgggtttggg attggtgccg ccgctagagc tagaagagct
      241 ggttgtgccg cctgaggagc tggtatttgc cgaaccggtc gagctggagc tgctagaact
      301 tgagctgctg gagcttgagc tgctcgacgg gttgggcgga ttctgcccgc cattgttttt
      361 tgacgaacct ttacctgatc cacaggcggt aaggccgaca attaaactaa gtgtaagaat
      421 gggcaggcgc atgaataaat ctccatcaga ttttcggggt tgagcaattc taaaccagtt
      481 ggtagctatg gttccgctaa ccatttccgt ttttagcatt ttcgcaggta ctgttgagca
      541 tttttgaagt gaattgcaac aaaaattttg tgattacttt gctcgtcagc ccacgtcaaa
      601 agcctatttg catacgcagg gcagttatga atggttgtga cagaaggtgg ataataagat
      661 aacgcgttag ggtaaatttt gtttttgcct ttatttgcag cgctttttta attttctgtg
      721 ttgagatgag cgagcgctaa tacctatgga ggtggtatta ggaagaaagt gtgtattttt
      781 gtataaaatt cctcagatgg gatgaggggg caaaacgctt tagtgggatt cttgttgcgt
      841 gctctcaagt aaggacaaac ttacactgtt ttttatgcgg ctagctgcag cgctctgcca
      901 agagcattta tatgatatgg ataaatataa tttaatcgga ctagtctgag tgcttttttt
      961 aaattttcta agggtaaatt taatactaaa taattagtgt tgaggcttga gtatccataa
     1021 gcagtttgaa ccactggtgt ctcggcgcaa tcccctgcca actcccgcaa gaaatgctac
     1081 ctcggtcgga atgaaccggc tgcagactta attctgttct attcctctat atatgtcggt
     1141 agcggttgcg cgcctaactt cgcccgttcc tcccttcatg gctgcagatg cctataaaca
     1201 gaattccgcc atcagataca ggattccgac atcaggctct gccacttgct tagtcccggc
     1261 tgccagttta tttaggcttt agtcgtggtt ttggcagtga acctgcatag ctcagacaag
     1321 tgcagtggtc atccgagggt tgcccagaat caccgctcaa gtagtgcgtc ttatcagagg
     1381 aggtgccaca tcctagctat aatttgtagg gacttcctgt atttcgtaac gccctgctgc
     1441 tttatttttg cctttgtccg ccacacggat aattcaaata ggatcctttt atgccccatt
     1501 tgctgaccgg ttacgccctg cttgccaatg ttaaatacag taaaggaacg gctttcagtc
     1561 tggaggagcg ggatgcctat ggattgcgtg ggttgttgcc gccacgggtg tgcaatatgt
     1621 cggtgcaagt gcagcgcgcc ctggctaatt tgcgtcgcaa aacctgcgat atcgaccgct
     1681 atgtgtttat gcagcagttg ctcacccgca gccaaacgct gttttaccgc gtattgatcg
     1741 accatatcga agagttaatg ccaattgtct ataccccgac tgttggccag gcctgccggg
     1801 agttcgccca tatctatcgc caggcccagg gattttatat ttccctggcg gaccgggggc
     1861 ttgtgcgttc gatgttgaat aactggccgg aaaaagacat ccgcatgatt gtggtcaccg
     1921 atggcgagcg tattttaggg ttgggggatt tgggcgcgaa cggtatggga attcccatcg
     1981 gcaaattagc gctttattgc gcctgcgcgg gggtgcgccc ggagcagtgc ctgccggtga
     2041 tgattgatgt gggcaccgat aatgtaccgc tgcgggagga tccgttttat ttggggacgc
     2101 cctgcccgcg ggtgcgcggg gaagcttatt tggcgttggt ggatgaattt gtacaggcgg
     2161 tgcaggatgc ctatcccaag gcgttaattc agtttgaaga ttttctcacg cctaacgcct
     2221 atatgttatt gcaccgctac cggcgcgaag ttttgtgttt taacgacgat attcagggta
     2281 ctgcggcggt ggctttagcc ggtatttacg gcgcctgcac gcttgccaat ttggcgttta
     2341 atcagctgcg tattatgttt ttaggcgcgg gttctgccgc tacgggtatc gccgatttat
     2401 tagtgcaagc cttggtcgag caggggttaa cgcagcaaga agcgcgcggc cgtttatggt
     2461 ttgtggatgt ggacggcctg gtggtatcaa gccggcccta tcttgccgaa cacaaccgcc
     2521 cctacgcgca ggattatccg cctatggatt ttatatccgc cattgatgct attcgcccgc
     2581 aggtattaat cggtgctacc ggcgctgcta atgcatttac cccggacgtc attggccgta
     2641 tggcgcaata caatgtgcgg ccgattattt ttgcactgtc taaccccacg gcgaatgccg
     2701 aatgtaccgc cagccaagcc tatgaatgga gccagggcag ggcgatattt gccagcggca
     2761 gcccgtttcc tccggtcact tatttaggcc aatttttccg gcccgcccag ggcaataatg
     2821 cttatatatt ccccggtgtg ggtttggggg ccatgttggt ggaggcgcgt ttagtcagtg
     2881 atgaaatgtt tttgcgagct gcgcgggtat tggccgattg tttatcgccg atggatttag
     2941 ctgagggggc tttgtaccca cctttaaaca aaattcgcga cgtgtccctc gctatcgcct
     3001 gcgcggtggc tgaggttgcc taccaagaag gggtggcgcg gctgccccat ccgggggatt
     3061 taaaaacatt tattgccgag cgtatgtacg accctaatta tgaagagctg aaataaatag
     3121 ggggcgttat ttattgttgc acctaaaaat tagcgagcct ctgattcagt ttgcaggcgc
     3181 aggttgaacc gtagcgggca ggctgctgcg gcgtaataag cggctagggt ttttcagcga
     3241 taaaaattgc ccggcgcggt gcgggataac cttcgatggt gaggttagga tcatccgggt
     3301 gtaaaaaatc ctgcagagaa ttaaagtgca tccagtcggt agtgcgctgt tcgtcgcagc
     3361 gggtttggtt gatatcaaca cagcgggcat ttttaaagcc tattttagcc agccagttta
     3421 aaagtgtcgg tacgctgggg ataaaccaca cattgcccat ttgcgcataa cgctcgctgg
     3481 gcactaataa ttggccgtgt ttttctggaa taatcagcgt ttctaaaatt aattcaccgc
     3541 cggggcgcag cacatcgcgc aattcccgca ggtgatccat gggtgagcgg cggtgataaa
     3601 acacccccat ggaaaaacag ctgtcaaagg cgtgtaaatt tgcaggcatg ctatcgaggg
     3661 taaccggcaa tacatccacg ggtgcctggg gccagtattt tttcagcata taaaactgca
     3721 ccacaaaacg cggcgacggg tctatgccga ttacttcgct ggcgcctgcg ccgtacatgc
     3781 gccaggcgtg ataaccgctg ccgcagccca catctaaaac ccgcctgccg gttaaatcgc
     3841 tgatgtgcgg taacacgcgg tgccatttcc aatccgagcg ccactcggta ttaattgtga
     3901 tgccaaataa attaaatggc cctttgcgcc agggcattaa attttttaat tggcttaaca
     3961 gctcagcgtg tttggcggga gcaatttcat tgccgcgacc tatttctacg gcatttaagg
     4021 catggcgata gtcggtggtt aattccggca gcgcagtgta agcggtaagc cagcgattta
     4081 aatcggcgtg gcgtttgata tctaaaccct gtgcaatctg ctctggcaat attttggccc
     4141 agggggcgag ggggccatcg ctaatacggg tgagaaatgc gcggtagtca atcatggcgt
     4201 catcagaata aataggtgta atcagtgcga tgggatttat ttaaaggcca gcatggaggc
     4261 gaaattaaaa cattgaaacc ataaatccgt actgcgaaac ccggcttggt ttaagcgctg
     4321 tttgtgggtg gcgtaggttt cgggaattaa aacgtgttca agtgctgtgc gcttttgggc
     4381 gatttctaaa tcggagtagc cgttggcgcg tttaaaattg tggtgcagtt gggtcattaa
     4441 ttcctggtgt tccgcatcct caaaggcgag tttttccgac aggatcaaca cgccgccggg
     4501 aattaggcca gttgcaatgc gctgcaaaat attcagccgc tcggccaccg gaataaattg
     4561 cagggtgaaa ttcagcacca ccaccgaggc gttgtggatg ggaatatctt gcaggttggc
     4621 gtggtgcagg ctgacgggca cttcacccgc atcggcggcg attacctggc gggctttttc
     4681 caccatcgcg ccggaattat ctacggccac aatttgcgtg ccgctggtat ttatgcggtg
     4741 gcgcatggcc agggtggcgg cgccgaggga acagcccagg tcataacagt aggtattggg
     4801 gcgggcgtag ccttcggcga tctggccgat catacgcaga atggtggcat agccgggcac
     4861 cgaccggttg atcatatcgg gaaaaacatc caccaccgtt tgatcaaaac aaaaatcgcc
     4921 caaatgggtg aggggattgg caaacagtgt gtcggttttg ctgtgcatgg cgagcctgcg
     4981 ggcggggtgg gcggcaatca tactccggca gtgggcagcg tcaaaggtca gctacactta
     5041 gggcgaaggc atatgttaac tgcgggtatt ggtgtgcacg ccggttgggt gcaataccgc
     5101 agtgggccga ctaggaggtt tatgggtgat attgattacg gcagcatgcg ctgcttggtg
     5161 gtagatgatt ttggcaactt tcgcacaacc cttgccgcta tgttaagcaa aatgggctgg
     5221 gtgcaggtag accaggccag caatgttacg ggcctaatcg ccgcctgcga aaaacgccaa
     5281 tacgatttaa ttctcaccga ttacgaccta ggccccggcc gcaacggcca gcgggggcta
     5341 gaggagctgc ggcatcgcaa gttaatcagc aagcgcaccc tgtttgtact ggtgtcaggc
     5401 aacgccagca aagaggcggt actggccacc tacgataacg agcccgacga ttacctcgcc
     5461 aaaccggtta ccgccaaggt gctgcaacag cggctcgacc gattattcgc tcagcgccag
     5521 gtattactgc cggtgtataa agccctggat aatggcgatg cggcccgcgc ggtatttcta
     5581 ctcacagaaa tgtccatcgc caataaccgc cactctatcg ccgcccaaaa agccctgggg
     5641 cgtttgttca ttgaacaggg cgagctggat aaagccgaac gggtgttttc cctcgcactg
     5701 caaactcggc cgctggattg ggcgcggctg gggcttgccc gggtgaaggc cctgcggggg
     5761 gaaaaagcca tcgctggtga atggatggag cagattatct ccgacagccc gctgtttttg
     5821 cccgcctatg atgccctggc agatacctgt gccgatgctg ggcagcgcca agtggagcag
     5881 gcgacggtac aaaaagccgt gaatatttcg cctatgtcga tcctgcggca aaagcgctta
     5941 gcggaagtgg cggaggccaa tggcgattgg ctcacggcca tgcgggcctg gcggcgcgcc
     6001 atcaaattgg gcgaggagtc catccacgga gaggcccgcg atggcattaa attcgcccgt
     6061 gccgtggcc
//
LOCUS       DAOQGS010000225         6048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6522_length_6048_cov_5.037711, whole genome shotgun sequence.
ACCESSION   DAOQGS010000225 DAOQGS010000000
VERSION     DAOQGS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6522_length_6048_cov_5.037711"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PKZ52_09625"
     CDS             complement(<1..1003)
                     /locus_tag="PKZ52_09625"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNG60075.1"
                     /translation="MKDNSIPGQWVNISIGEIADVVAGGTPSANNPDNFATAGSGIAW
                     LTPADLSGYKAKFISHGARDLSQTGYSTSSAKLLPAGSLLFSSRAPIGYVAIARNDIS
                     TNQGFKNFVFPAEVNPSYAYYYLRSIRDLAESLGTGTTFKEISGATAKTLPFILSPLA
                     EQKVIAEKLDTLLAQVETTKARLERIPEILKRFRQSVLAAAVSGRLTEDWRLENSTNA
                     VQPTIDKHNSQKNGLLKVRGQSSWDTNIDLYDLPENWAWIENHKLATDEGNAICAGPF
                     GTIFKAKDFRDKGVPIIFLRHVKESGFNQNKPTYMDKEVWKELHQEYSVHGGELLVTK
                     "
     gene            complement(1000..2574)
                     /locus_tag="PKZ52_09630"
     CDS             complement(1000..2574)
                     /locus_tag="PKZ52_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNG60076.1"
                     /translation="MTHNDIVQKLWNLCDVLRDDGINYSDYVTELVLLLFIKMVHEQT
                     QSETLKKHPLPTGCRWTDLNNKSGINLLNDYKRILLALSTGKDAQGKLVHDDPLISAI
                     YADAQTRLREPRHLEQMIKTLDQIDWFSAAKDGLGDLYEGLLEKNASETKSGAGQYFT
                     PRPLINSMVRCLKPQAGELIQDPAAGTAGFLVAAHEFIKANTDDLYTLSPRERNFQKN
                     KAFIGVELVPNTRRLALMNCLLHGLEGDDEGVVLRGNSLGDVGKALNPADMILANPPF
                     GTSKGGDASITRDDLTFDTSNKQLAFLQHIYRNLKPGGRAAVVLPDNVLFEAGKGTDI
                     RRDLMHKCNLHTILRLPTGIFYAQGVKTNVLFFTKGSAKDPHHDENCTQQVWVYDLRT
                     NMPSFGKRTPFTEEHLKPFEKVYGKKADGTSKRTEGEYSFGAEQVELDKTAENTGVDE
                     HLAHSRWRCFSRDWIANTKGDSLDISWLKDKDSVDAANLPEPEVLAREAKGELEAALV
                     ELDGLLKALGEGGNAQ"
     gene            complement(2658..3872)
                     /locus_tag="PKZ52_09635"
     CDS             complement(2658..3872)
                     /locus_tag="PKZ52_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNG60077.1"
                     /translation="MSIICKFFDTKTCYVDPIDINCPAHPNVSKNSGTLTMTWQADLP
                     YNDLPPLPPARQQIETVPILKACITARAALAELKQAGQLLPNQTLLINLLPLLEAKDS
                     SEIENIVTTMDKLFQFAHEDTAADAATKEALRYRTALYQGFKQLQQRPLSVTTATEIC
                     STLKAQTMEVRKIPGTVIANQQTREIVYTPPVGEETIRRLLGNWEQFFHSDDDLDPLI
                     KMAISHYQFEAIHPFLDGNGRTGRIVNVLYLIEQQLLTLPILYLSRYIVQHKPDYYRL
                     LTQVTREQDWQAWILFMLQGVQEVSVWTCAKIAAIRRLLEHTSEYLRTSLPRIYSHEL
                     IQLIFEQPYCRISDLVERDLAKRQTASVYLKQLCETGVLQEQSAGKEKLFVHPKLITL
                     MTQDNNNFTPYT"
     gene            3987..4898
                     /locus_tag="PKZ52_09640"
     CDS             3987..4898
                     /locus_tag="PKZ52_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNG60078.1"
                     /translation="MQEVKISASVGENAKNNKQDVIEIQKALNRIIVHNVLVPLAPLK
                     VDGIAGSNTKTAIRQFQRVSVGAASPDGRIDVGGRTIKKMNEVLAVCDKVKDKTGSKS
                     IRWTIGVDLDHLRTETYRTFNMVKQNVLAGETLHKAQAGNVVPITNKNLGILSKEEFT
                     KEVFASAKIEEAKSKIPAALTTAQAILESAFGKKVPTDLYTKQYSYNLFGVKGVGPAG
                     SVSVFTHEVKGGVRIGIVDKFEAYFSFEGSIAGRTAFLKRNRRYRELFELTDPVEWAE
                     GLQKKGYATDPNYAKALIEIMKCRKLI"
     gene            4895..5545
                     /locus_tag="PKZ52_09645"
     CDS             4895..5545
                     /locus_tag="PKZ52_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60079.1"
                     /translation="MKIFPIVIIFASFFASACNAEAPNKQINSKPVEATSPAISSVKP
                     VDQASIESTLGDFSKAIATKDTKLFVATTNSKSVFLVRKFASGTLGGRGSELSAEFEP
                     SSIAKDMSFAIKNQTSLELPSVFVTQPIQSYSSLKQYPLPPEIDSTHYDQWAPHLLKA
                     LTNKPEAGPGDPVILKSAKYWIYAEAQIIDGVLVGSFAAFFNENNRATLGAIVVFL"
     gene            complement(5739..>6048)
                     /locus_tag="PKZ52_09650"
     CDS             complement(5739..>6048)
                     /locus_tag="PKZ52_09650"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNG60080.1"
                     /translation="IAASIIGHIRQAAIGEALIPFEQRVARALQGIYQSQAWSPTQRK
                     WLERLAKQLVHEVVIDQSFVNDVFGDDGGAKQLNKLLNNKLDEVLEAINDGLWVQAGP
                     "
BASE COUNT         1455 a         1335 c         1526 g         1732 t
ORIGIN      
        1 gcttagtaac aagtaactca ccgccgtgaa ctgaatactc ttgatgtaat tccttccaaa
       61 cttccttgtc catatatgta ggtttgtttt gattgaatcc tgactccttc acatgtctca
      121 gaaatattat tggtacacct ttatctctaa aatctttggc cttgaagatt gttccaaatg
      181 ggcccgcaca tattgcattt ccttcgtctg ttgctagttt atgattttcg atccaagccc
      241 aattttctgg aagatcgtat aggtcgatat ttgtatccca gctcgattgt ccccgaactt
      301 ttagcaatcc atttttttgt gaattgtgct tatctatcgt tggttggact gcattggttg
      361 aattttctaa gcgccaatcc tccgtcaacc tcccactaac cgccgccgca agtacggatt
      421 ggcggaagcg tttgaggatt tcggggatgc gttcgaggcg ggctttggtt gtttctacct
      481 gggctagtag ggtatctagt ttttcggcga tgactttttg ttcagctagg ggggagagaa
      541 taaaaggtaa tgtttttgca gttgctccag aaatctcttt aaaggtcgtt cctgtaccta
      601 gactttccgc taaatctcgt atgctccgca gataataata tgcatagctt ggatttactt
      661 cagctggaaa tacaaaattt ttaaatcctt gattcgttga aatatcgttt cttgcgattg
      721 ctacatatcc aattggggct ctgctgctga acaacaaact accagctggc aataatttgg
      781 cagaacttgt gctatatcca gtttggctga ggtctctggc gccatggctt ataaatttgg
      841 ctttgtaacc gcttaaatca gcaggtgtca gccacgcgat tccactgcct gctgttgcaa
      901 aattatcggg gttgttggcg ctcggagtgc cgcctgcaac tacatcagct atttcaccaa
      961 tagatatatt tacccattgg cctgggatag agttgtcttt cattgagcgt tgcctccttc
     1021 tcccaatgcc ttcaacaacc catccaactc caccaaagcc gcctccaatt cccccttagc
     1081 ctctcttgcc aacacctccg gctccggtaa attcgccgca tccacactgt ctttatcctt
     1141 aagccaggaa atatccaaag aatccccttt ggtattggca atccaatccc ggctaaaaca
     1201 gcgccagcgc gaatgggcta aatgctcatc cacaccggta ttttcagcag ttttatccaa
     1261 ttccacctgc tcggcaccaa aggagtattc gccttcggtg cgtttgctgg tgccgtcggc
     1321 ttttttgccg tagacctttt caaagggttt taggtgttct tccgtaaaag gtgtgcgttt
     1381 gccgaagctg ggcatattgg tgcgcaggtc gtacacccat acttgttggg tgcagttttc
     1441 gtcgtggtgt gggtctttgg cggagccttt ggtaaaaaac aacacattgg ttttaacgcc
     1501 ttgggcgtaa aaaataccgg tgggcaggcg caagatggtg tgcaaattac atttgtgcat
     1561 taaatcccgg cggatatccg tacctttacc cgcttcaaat aaaacattat ccggcagcac
     1621 cacggcggcg cgcccgccgg gttttaaatt gcggtaaata tgctgtaaaa acgccagttg
     1681 tttgttgctg gtatcaaagg ttaaatcgtc ccgggtaata ctggcatcgc cacctttgct
     1741 ggtgccaaag ggagggttgg ccagaatcat atcggcaggg ttcagcgctt tgcctacgtc
     1801 ccctaagcta ttgccgcgca gtaccacgcc ttcgtcatcg ccttctaagc cgtgtaataa
     1861 acaattcatt aaagctaggc gccgggtgtt ggggacgagt tctacaccga taaaggcttt
     1921 gtttttttgg aaattgcgct cgcgggggga gagggtatag aggtcgtccg tattggcttt
     1981 aataaattca tgggcggcca ctaaaaagcc tgcggtgcct gcggctgggt cttgaattaa
     2041 ttccccggct tgcggtttta aacagcgcac catgctgtta attaaggggc ggggggtgaa
     2101 gtattgcccg gcgccggatt tggtttcgct ggcgtttttt tccagcaggc cctcgtataa
     2161 atcccccagg ccgtctttag cggcgctgaa ccaatcaatt tggtccaggg ttttaatcat
     2221 ctgttctaaa tggcgtggct cgcgcaggcg ggtttgggca tcggcataaa tagcgctaat
     2281 tagtgggtcg tcatgaacca acttgccttg ggcatctttg ccggtggaga gggcgagcaa
     2341 aatgcgtttg taatcgttaa gcaggttaat gccggatttg ttgtttaaat ccgtccagcg
     2401 gcagcctgtg ggtaaggggt gtttttttag ggtttccgat tgggtttgtt cgtgcaccat
     2461 ttttataaat aacaataata ccagctcggt gacgtaatcg ctgtaattaa tgccgtcgtc
     2521 gcgcagcacg tcgcagaggt tccagagttt ttgcactatg tcgttgtggg tcattgattt
     2581 gccttgttta aaaagctgtc ttgtttaaaa agctgcttag tgtaagtagt tgcctggggc
     2641 aggtgtaatt tgggggccta ggtgtaaggt gtaaagttgt tgttgtcttg ggtcatgagc
     2701 gttatcagct tggggtgcac aaagagtttt tctttgcctg cagattgctc ctgtaacacg
     2761 ccggtttcac atagctgttt taaatacacg gaggcggttt ggcgtttggc cagatcgcgc
     2821 tctaccaggt cgctgatgcg gcagtagggt tgctcgaata tcagctggat gagttcatgg
     2881 ctgtagattc tgggcaggct agtgcgcagg tattccgagg tgtgttccag caagcggcgg
     2941 atggcagcga ttttagcgca ggtccaaacg gatacctctt gcacaccctg cagcataaat
     3001 aaaatccagg cttgccaatc ctgctcgcgg gttacttggg tgagcaggcg gtaataatcc
     3061 ggcttatgtt gcactatata acggctgagg tagaggattg gcagggttaa taattgctgt
     3121 tcaatcaggt aaagcacatt aactatgcgc cctgtgcggc cattgccgtc taaaaacggg
     3181 tggatggctt cgaattgata atggcttatg gccattttaa tcagcgggtc taggtcatca
     3241 tcgctgtgga agaactgctc ccaattgccc agcaacctgc gaatagtctc ctcccctaca
     3301 ggcggtgtat aaacaatttc acgggtttgc tgattggcga ttacggtgcc gggaattttg
     3361 cgtacttcca ttgtttgcgc tttaagggtg ctgcagattt cggttgcagt ggtaacgctt
     3421 agcgggcgct gttgcagttg cttaaagcct tggtacaggg cggtgcggta gcgcagggcc
     3481 tctttggtgg cggcatctgc ggctgtgtcc tcgtgggcaa actgaaatag cttgtccatg
     3541 gtggtgacta tgttttcaat ttctgaactg tctttggcct ctagcagtgg caacaaatta
     3601 atcagcaagg tttgattagg cagcagctgg cctgcctgct tgagttcggc cagggctgcc
     3661 cgcgcggtga tgcaggcctt gagaattggt accgtctcga tctgctggcg cgcaggtggc
     3721 agcgggggca gatcgttgta gggcaaatcg gcttgccagg tcatggttaa agtacctgaa
     3781 tttttcgaca cgtttgggtg ggcagggcag tttatgtcga tgggatcgac ataacaagtt
     3841 tttgtgtcga agaatttgca gattatcgac atgtcgcttc ctgggcaaac caactggctg
     3901 ctgttcatgt tttatgggtg gccttgcttt gctagcgcat cattcataga atcgtcgctc
     3961 gggactaatt aatcaggagc tgcagtatgc aagaggtaaa aatttccgcg tcggttggtg
     4021 agaacgcaaa gaataataaa caggacgtaa tagagataca aaaagccttg aacagaataa
     4081 ttgttcacaa tgtcttagta ccgctggcac cgctcaaagt agatggtatc gcaggttcca
     4141 atacaaaaac ggcgatcagg caattccaac gcgtatctgt cggagctgcg tcaccggatg
     4201 ggcgaataga cgtaggcggc agaactatta aaaaaatgaa tgaagtttta gcggtttgtg
     4261 acaaggtaaa agataaaacc ggctcgaaat ccattcgatg gacaatcggg gtcgacttgg
     4321 atcacctgcg cactgaaacg tatcgcactt ttaatatggt taagcaaaac gttttagcag
     4381 gagaaacctt acataaagcc caggcaggca atgttgttcc tatcacgaat aaaaatcttg
     4441 gcatcttatc caaagaagaa tttactaaag aggtatttgc cagcgcaaaa attgaggaag
     4501 ctaaatccaa aatccctgca gctcttacca cggcgcaggc tatattggag tctgcatttg
     4561 gtaaaaaagt ccctaccgat ctatatacca aacagtacag ctataatctt ttcggcgtca
     4621 aaggggtggg gccggctgga tctgtctctg tgttcactca tgaggtaaaa ggtggcgtta
     4681 ggattgggat agttgataag tttgaggctt attttagttt tgaaggatca atagctggca
     4741 gaacagcctt tctaaagaga aataggcgtt atagagaact ttttgaattg actgatccgg
     4801 tggagtgggc tgagggtttg cagaagaaag gttatgccac tgaccctaac tatgctaagg
     4861 cgctaattga aataatgaaa tgccggaagt taatatgaag atattcccta ttgtaattat
     4921 atttgcatcg ttttttgcga gcgcctgcaa tgccgaggca cctaataaac aaataaacag
     4981 taaacccgta gaggcgactt cgcctgctat atcgagtgta aaaccggttg atcaggcgtc
     5041 aatcgaatct acgctgggtg atttttctaa ggctattgca accaaagata caaaactgtt
     5101 tgttgcgacg accaattcaa aatcagtatt tttagtgcgc aaattcgctt caggaacgct
     5161 cggcggtcgt ggatcagaac tcagtgccga atttgagcct agctctatcg caaaagatat
     5221 gtcttttgcc ataaaaaatc agacttcgct agaactgcca tcggtttttg tcacccagcc
     5281 gatacagtct tattccagtc tgaaacaata tccgctacca ccagaaattg atagtaccca
     5341 ttacgatcaa tgggcgccgc acttgctcaa ggcattgaca aataagcctg aagcaggccc
     5401 gggggaccca gtaattttga aatcagcaaa atattggatt tatgctgaag cgcaaattat
     5461 tgatggagtt ttagtgggca gttttgctgc gttttttaat gaaaacaaca gagctaccct
     5521 tggtgccatc gtggtgtttt tgtaatagtg catcgatagt tgcctaagtg gcctcaccaa
     5581 tgcctgttaa aggtttatgg ggcggaagca gtttttaagt ctaatacgct agtatttttt
     5641 ccctttttct ttcatttcag cgccccacag tggccgggcg ccctagccct cctcttagaa
     5701 aaggagggcg cataagacct ttaataataa gttcaatttt acgggccagc ttgcacccaa
     5761 agcccatcgt taattgcctc caacacctca tccaatttat tgttcagtaa tttattaagc
     5821 tgtttggcac cgccatcatc accgaacacg tcgtttacaa aactctggtc gatgaccact
     5881 tcatgtacca attgtttagc tagccgctct aaccatttgc gttgggtggg cgaccaggct
     5941 tggctttggt aaataccctg taaagcccgg gcaacccgct gctcaaaggg gattagtgcc
     6001 tcgcctatag cggcttggcg gatgtggccg atgatgctgg cggctatg
//
LOCUS       DAOQGS010000226         6004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6585_length_6004_cov_4.592368, whole genome shotgun sequence.
ACCESSION   DAOQGS010000226 DAOQGS010000000
VERSION     DAOQGS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_6004_cov_4.592368"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKZ52_09655"
     CDS             <1..598
                     /locus_tag="PKZ52_09655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNG60081.1"
                     /translation="ATPFTITGSIGVFGMFPTVDEGLNKLGIFTDGVATHALSGAMRP
                     DRPMDPTAEKIAQQGVESIYQRFLSLVSEARKRDTAEIDAIAQGRVWTGGKAQALGLV
                     DQLGYLNDVVKAVAEQQKITTPKLKVIQRDLSAQEQLIRNWVEQSSHVQTGPLSQWFT
                     RISNLLSQPALGQFNTFEQAAKPYGFSIMAACLDCSAL"
     gene            complement(761..1369)
                     /locus_tag="PKZ52_09660"
     CDS             complement(761..1369)
                     /locus_tag="PKZ52_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNG60082.1"
                     /translation="MSGISLIALALCCAWASGINVYAVLLVLGLSGGFNYLLLPDGLE
                     VLTHPLVIMFFGFMYCVEFVTDKIPGVDSLWDFVHTFIRIPVAAFLVGALFEGVYPDW
                     QLVAQTLAVVLALSSHISKASSRLLINTSPEPFSNWAASCGEDILVFIALWLAFAHPV
                     VFCLVLLGFVALIIWLLPKIIRLLVAVIVKLRNWLTSSNLAT"
     gene            complement(1742..3445)
                     /locus_tag="PKZ52_09665"
     CDS             complement(1742..3445)
                     /locus_tag="PKZ52_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNG60083.1"
                     /translation="MKALVVDDNAFNRDILSYILEDAGHQVVQANDGSEACHIFERQT
                     DIEFILMDVNMPVMDGFEATQIIRAMAGDKPVSIIFVTAWDNPDITVKCLEVGGDDFV
                     PKPINEQILLSKIKAHQRSQSLYLNLKRTNEDLLYHQCMVEREHKIVEHVFAQCAQRQ
                     TTFCKNVQQHSSPSSMFNGDLVLTAPAPNGGVYVLAGDFTGHGLSAAIGSLPVTEIFY
                     DYARRQRSIAQMAREINKRLHTLLPIGMFFCCTLLFIDPQGQQCMVWSGGMNDLVVCP
                     EGNSNLQFLQAAHMPLGILEDEEFDDLIHIHEFGLGARLYIYTDGINEATDSAGQVLG
                     HEPVWAVIRQGKVGQTLDKIVELVKTFEGDTTQHDDFTLVEVLFTPLIHVDKTTHEPV
                     DIAAEHLRVSSFPWRLTARLNADDMRRTDMIQQILAFIGTIQGVELHKDKLFLIASEL
                     YNNALEHGVLKLSSELKNTVGGFGEYYKQRSERLASLESGYIDVDFTFVRGDPNEVHM
                     VVSNSGEGFHFRQYFPKEPDGDTHAGQNHGRGIKLLRQLCKSLDFDNGGCTAKAIYQF
                     Q"
     gene            complement(3673..3972)
                     /locus_tag="PKZ52_09670"
     CDS             complement(3673..3972)
                     /locus_tag="PKZ52_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNG60084.1"
                     /translation="MKIEKKSTEEVQIVLDEVFDFKKAEEFRLLYEGIACAGVKVVTI
                     NFLAARYMDSSGLGMLLNVQSYFKDKKIVVTLANPNEQIRKIFSISNFDRRFTIL"
     gene            complement(4159..5319)
                     /locus_tag="PKZ52_09675"
     CDS             complement(4159..5319)
                     /locus_tag="PKZ52_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG60085.1"
                     /translation="MTDTTLRLVVYLGISLVSCGLVYATDSLYLAMAALLILGAMAAY
                     GVAQVPSLGQSVQVHNQNRANDLLHQTHQVFTDKHRRTCDQINLVAHDIRDVVERNSL
                     ALHSSFQSLSANAIAERDILESVSAHLSTGAAEKDHAKTVSLAHFANDMGRILDDYVR
                     LFLDVSSKSIQAVHKIQDMVKHLDGMFGLINEIRGIADQTNLLALNAAIEAARAGDAG
                     RGFAVVADEVRKLSKDSNELNDEIRQKAQKAKETVTQVESVVGEIASMDMNLAIDAKG
                     HLDSMLAELEKMNRTVSDGVAKGSQIGDTMQQEVSRAFAALQSADQISQMLRQVTNCT
                     EFLYSIQQALAADEHGRNLEDSLEHMLKRLKALSEAPDVRMDVKNVGGIELY"
     gene            complement(5490..>6004)
                     /locus_tag="PKZ52_09680"
     CDS             complement(5490..>6004)
                     /locus_tag="PKZ52_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNG60086.1"
                     /translation="YGRVSLLIKNMPVDDPEKYGAIKDNTFSLLQGLNARILALEDRC
                     GLLEEKESLLKISQDVNAVMSSIKQSYQAVMRQILTEVENAVEMIEHRLPHLALNEVD
                     ESFMFSVTDQLVKETNEVFNNGLKVDEIFERLEQALQRAVNTVDKESIKQELGSKSSQ
                     RGPDKSVELF"
BASE COUNT         1697 a         1530 c         1244 g         1533 t
ORIGIN      
        1 ggccaccccc tttactatta ccggctctat cggcgttttt ggcatgttcc caaccgtcga
       61 tgaggggctc aataaactgg gcatttttac cgatggagtg gcaacccacg ccctctctgg
      121 cgccatgcgc ccggatagac ctatggaccc aacagcagaa aaaatcgccc agcaaggggt
      181 tgaatccatt taccaacgct ttttaagttt ggtgagcgaa gcgcgcaaac gcgatacagc
      241 ggaaattgac gccattgccc agggccgcgt gtggaccggc ggcaaagcac aagcactggg
      301 tttggttgat caattgggtt atttaaatga cgtggtcaag gctgtagccg aacaacaaaa
      361 aataaccacg ccaaaactta aagtgatcca gcgcgatttg tcggcccaag aacagcttat
      421 tcgcaattgg gtagagcaat ccagccatgt gcagactggg ccactaagcc agtggtttac
      481 gcgtatcagc aacctacttt cacagcctgc gctagggcaa tttaatacgt tcgagcaggc
      541 cgccaaaccc tatggcttta gcattatggc ggcctgtttg gactgttcag cgctttaaat
      601 acttcacagc ctgttgaaac atgtactgaa aggtaagaac ttcaacaggc tggactgtgc
      661 attgtttttt tgccacgcac tagcgagcac tgcaaagctg cggtgccatt aaccccaata
      721 agccgccgag ttaatactct ggaatccact tttcgcgccc ctaagtcgca agattactgg
      781 atgttaacca attgcgcagc ttaacaatca ccgccactag cagcctaatt attttcggca
      841 gcagccaaat aattaaagcc acaaaaccca acaacactaa acagaatact actgggtgcg
      901 caaaggcaag ccagagggca ataaacacaa gtatatcttc accacaggag gcggcccaat
      961 tactaaaagg ttccggcgat gtgttaatca ataagcgcga agaggctttg cttatgtggc
     1021 tagatagcgc caatactact gccagtgttt gagcaaccag ctgccaatcc ggataaaccc
     1081 cttcaaatag agcccccacc aaaaatgccg ccactggaat acgaataaat gtatgtacaa
     1141 aatcccacag gctatccacg ccgggaattt tgtcggtaac aaactccaca caatacataa
     1201 aaccaaaaaa cataatcacc agcggatggg tcaaaacctc aagaccgtcc ggcagaagca
     1261 gataattaaa tccaccgctt aaccccaaca ccaataaaac tgcgtacaca ttaataccgc
     1321 ttgcccaagc gcaacaaaga gccaaggcga ttaaggaaat accggacata taagcctccc
     1381 attattaacc cagcggcaaa atagatcgtc ctgatctatt ttgccttcgc tgcactaaac
     1441 ctaagaaaat gcgcggattt tcttaggttt agtgcggctc gcggcggttc tcacctttga
     1501 tgcggcatcc atgccgcctt tattaacccg gcactaattg ccgccgggtt aataacttaa
     1561 tactattgag tgattagggg caatgtcttt atcagcgaaa gcctgcccat gggctgcaag
     1621 tttacacgat tgacttaagg atgcgcagga aaaaccgcga ggaccgagga gtataagcaa
     1681 ccggcactat cttaagcggt aaatatattg gccagcagcc gaaatttagg caagcaagga
     1741 attattgaaa ttgatatata gctttagcgg tacagccgcc attatcaaag tccagtgatt
     1801 tgcagagctg gcgtaacagt ttgatacctc ggccatgatt ttgcccagca tgggtatcac
     1861 cgtcgggctc tttagggaaa tattgcctga aatggaagcc ttcaccggaa ttactgacaa
     1921 ccatatgcac ctcgttggga tcaccgcgca caaacgtaaa atccacatct atgtaaccgg
     1981 attccaagct agccagtcgt tcagagcgct gtttatagta ttctccaaaa ccaccaaccg
     2041 tatttttcaa ctcagatgat aatttcagca cgccgtgctc tagtgcatta ttgtaaagct
     2101 cgctggcaat taaaaataat ttgtccttgt gcaactcaac accttgaata gtacctataa
     2161 atgcaagaat ttgctgaatc atatcagtac gacgcatatc atcggcattt aacctagccg
     2221 ttaaacgcca gggaaatgaa ctcacgcgca aatgctccgc agctatatcc actggctcat
     2281 gggttgtttt atccacatga attaaaggcg taaatagcac ttcaactaac gtaaaatcat
     2341 cgtgctgggt tgtgtctccc tcaaaggttt tgactaattc tacaattttg tccaatgtct
     2401 gcccgacttt cccctgacga ataaccgccc aaacgggttc gtgccccaat acttgcccag
     2461 cgctatccgt tgcttcgtta atgccatcag tataaatata taaacgcgcc cccaaaccga
     2521 attcgtggat atggattaaa tcgtcaaact cctcatcctc cagaataccc aggggcatat
     2581 gggcggcctg caaaaattgc aagttagaat tgccttccgg gcacaccacc aaatcattca
     2641 tgccgcccga ccaaaccatg cattgttggc cttgcgggtc gataaataat agcgtgcagc
     2701 aaaaaaacat acctatgggc aacagagtat gtaggcgttt attaatttct cttgccatct
     2761 gcgcaataga gcgctggcgg cgggcgtaat cgtaaaaaat ttccgttacc ggcagggagc
     2821 caatcgccgc cgataaacca tggccggtaa aatctcctgc gagcacatat acacccccat
     2881 tgggcgctgg cgccgtaaga accaaatcgc cgttgaacat agaggagggc gaactgtgtt
     2941 gctgcacatt tttacaaaag gtggtttggc gttgcgcaca ttgtgcaaac acatgctcaa
     3001 caattttatg ctcccgctcc accatgcatt gatgataaag caagtcttcg ttggtgcgtt
     3061 ttagatttaa atagagggat tggctgcgct gatgggcctt aattttggac agtaaaattt
     3121 gctcattaat aggcttgggc acaaaatcat cgccgcccac ctccaaacat ttaaccgtta
     3181 tatcaggatt atcccaggca gttacaaaaa taatactcac cggcttatcc ccggccatag
     3241 cgcggataat ttgcgtcgcc tcaaagccgt ccattaccgg catattgaca tccataagga
     3301 taaactctat gtcggtctga cgttcaaaaa tatggcaagc ctcagagcca tcatttgcct
     3361 gcacaacctg atgcccggca tcttctaaaa tgtaacttaa aatatcgcga ttaaaggcgt
     3421 tatcatccac cactaatgct ttcactttac cccccatcaa tataggaacc tttgattaag
     3481 tctggggcgg ggatattttg aaaccgtcgc gggcaagaaa gcccacgact ggctgcaggg
     3541 agacactggt gctttttcaa aataataacg aattcagacc taatcaaagc atcctatgca
     3601 caagcattac cgcgccagac cgggcggcct agtcttaacg ggccagtttg ttactgttat
     3661 attcggtact ggctagagaa tagtaaagcg gcgatcaaaa tttgatatgg aaaatatctt
     3721 gcggatttgc tcattagggt tagccagagt cactactatt tttttatctt taaagtaact
     3781 ctgcacattg agcagcatcc caagcccgga agaatccata taacgcgccg ccagaaagtt
     3841 gatagtaacc acctttacac cggcgcaggc gatgccttca tagagcagtc taaactcttc
     3901 tgccttttta aaatcgaaca cttcatccaa cactatttgc acttcttccg tagatttttt
     3961 ttctattttc attcgcaacc taattagctg tagatcaatt tttatagact gaaaaaacta
     4021 acgcctaaat atccgttgac aacgggcagc agagcaaaga acacaggcgt tagggggcgc
     4081 agtagttaca gataaggcca cataaaatct tctaactgcg caggtgtatg tcaccatctc
     4141 actattttta gctggcagct aatataattc aataccgcca acatttttaa catccatgcg
     4201 cacgtctggc gcctcggaaa gggcctttag gcgttttaac atatgctcaa ggctatcctc
     4261 caggttgcgt ccatgctcat ccgccgctaa agcctgctga atcgaataaa gaaattcggt
     4321 gcaattagtc acctgacgca gcatctgtga tatttggtca gccgattgca gggctgcaaa
     4381 cgcccgcgaa acttcctgtt gcatggtatc gcctatttgt gatcccttcg caacaccatc
     4441 actgacggtg cggttcattt tctccagctc tgccaacatg gaatccaaat gtcctttggc
     4501 atcaatggct aaattcatat ccatggaggc aatttcaccc actactgatt caacttgggt
     4561 gactgtttct ttggcttttt gcgccttttg ccgtatttca tcgtttagct cgttggaatc
     4621 ttttgacagc ttacgcactt catccgccac gaccgcaaaa ccgcgccccg catcccccgc
     4681 acgcgcagct tcaatagccg cattcaaggc tagcaggtta gtttggtcgg caatgccgcg
     4741 tatttcatta attaacccaa acataccgtc caagtgcttc accatatcct gaattttatg
     4801 cactgcttga atacttttac tgctcacatc cagaaacaaa cgcacataat cgtccaaaat
     4861 tcggcccata tcattggcaa aatgcgctag cgatacagtt ttggcgtgat ctttttcggc
     4921 tgcacccgtg gagaggtggg cggaaaccga ctctaatata tcgcgctcag ctatggcgtt
     4981 tgcacttaaa gactgaaaac ttgaatgcag tgcaaggcta ttgcgctcga ccacgtcgcg
     5041 aatatcatgg gctactaaat ttatttgatc acaggtacgc ctgtgtttat cagtaaacac
     5101 ctgatgggtt tgatgcagca gatcattcgc gcggttttgg ttgtgcacct gcacagattg
     5161 ccccaagctt ggcacctgcg ccacgccata agcggccata gcgcccagta tgagcagggc
     5221 tgccatcgct aaatacagcg agtctgtggc gtataccaag ccgcaggaaa ccaggcttat
     5281 acctaggtag acgacaagtc gaagagtggt atcagtcatt agacatcctt aacagcaggc
     5341 caattgacgc gccctaaaat aataaagaaa tacaaaacag ctattcacga atacagttat
     5401 tttcttctgt gtacaacttt tatatcaatg ctttaaagct tttaatccac agagccaaga
     5461 aggatttcca aaataaacat aggcagaaac taaaacaatt caaccgattt atccggcccc
     5521 ctctgcgaac tcttactgcc caattcttgt tttattgatt ccttatcaac agtgtttaca
     5581 gcccgttgca aagcctgctc taagcgctca aatatttcat ccactttaag cccattgtta
     5641 aaaacttcgt tggtttcttt tactaactga tctgtcacac tgaacatgaa gctttcgtcc
     5701 acttcattta gagccagatg gggcagacga tgctcaatca tttcaacggc attttcaact
     5761 tccgttagga tctgccgcat taccgcttga taggattgtt tgatagacga catcaccgca
     5821 ttaacatcct gggatatctt aagtaaactc tctttctcct ctaacagacc gcagcgatct
     5881 tccaaagcga gaatgcgcgc attaagcccc tgcaacagtg aaaatgtatt gtcctttatt
     5941 gcgccatatt tctccgggtc atccaccggc atatttttaa taagcagtga tacgcgccca
     6001 tagt
//
LOCUS       DAOQGS010000227         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6609_length_5992_cov_4.010275, whole genome shotgun sequence.
ACCESSION   DAOQGS010000227 DAOQGS010000000
VERSION     DAOQGS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6609_length_5992_cov_4.010275"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3229
                     /locus_tag="PKZ52_09685"
     CDS             <1..3229
                     /locus_tag="PKZ52_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG60087.1"
                     /translation="DERIWLGLGRGGVTQFDEAKGKFTRFNYVYGTPGAPPFAAAMSL
                     FEDNNKNIWVGHYPGMVSFHDHSSEAISVYRKNNEGNKGISDNNVLGIAEDSHKNLWL
                     ALGEGVDYFDRQHASFKYYNNKLGNYPASGTLSAYLDRQSILWVGTWMEGFSRFNPAA
                     DRFEVMPFNATLANADERSGPQLHDSIVWGYCEDKNNNLWVGTHYAGINHYDAQQKQF
                     TKYNASVGGLANNIAWTCFEDSQGRFWVGTSDGLSLKDNKTGSFKNYRHEEGNANSLR
                     SGSVLDIYEDSKKRLWFATNDGLHLYRENSDDFEIFTADNGFINSGIRALTGDRQGNL
                     WLGTNNGIVRFNPDTLDVKNYLYFAGKKCGAVNTGAAMTSSAGEVIFGTTDGLIIIDV
                     EKLTTNQQQLPVVLTDFKVFAKSVSVDEPGSLLKKVINNTDKIVLDHTKKMVSFEFAL
                     LNYRSAYKNTYAYMLEGFDRGWREVGGAREAQYTNLSPGTYTFKVRAANNDGVWTSEP
                     KAIVLEQLPPPWKTWWAYTFYAVLIAVSIAYFVFLQKLKQRQVEEQNRLLEFKVAERT
                     RDLAEKNKDIQTLLANMRQGLLAIEEGGVIHHEYSAHLESIFETKTIAGQDVISFLFK
                     DALLDDDLVCQMRSGINSMIGEDEMNYVMNAHILIREYPLRIAEKIKILSLDWSPILD
                     AQGNVVRLMVSIRDSTELKALEHEAGVKRRELEIIGQLLPVSVKKFTDYSLSAQRYLS
                     ESLRLLESAGALDNALLNKLFRNIHTIKGNSRIHGFNYIANAAHEAETIFEQARSHNA
                     DQARLKVTEAIKRVEVALDDYKDIYRTLMLHNPPLSGRGDGVWVSRDGIEEIKTNAEQ
                     IKAVLPESAQSILTIIEKSSAAPLADIIAALVESLPEMAQSLGKQTPKVVISDAGILI
                     KEAVHERLRAIFTHLLRNALDHGIEPAAVRMAAHKDPAGTISIEVNLVNNALCIALRD
                     DGRGLDLNRLYHKGLELGLVDATAVNSPAKIANSIFIPGLSTKQTLDDISGRGVGMDA
                     VKQFLLEMGGDIAVELLDADVAQAEPKGVSFVFKLRVPLAVCIAE"
     gene            3728..5269
                     /locus_tag="PKZ52_09690"
     CDS             3728..5269
                     /locus_tag="PKZ52_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNG60088.1"
                     /translation="MERFRRSLWWIKRDIRLDDNPALQAALARSAELMPTYIMEPSLW
                     RAPDASAMQLAAVLQALACLQQSLQMRGSHLVVMQGECLVALDQLHAHYPFEAIFACE
                     ETGNNLTYRRDLALARWCRMRGIAFVELPQCGVVRAGNRASRSQIVHERLWQTPPVAA
                     PHHILSPAEGLPFNQADWLHDGRKQQAWLRLCPSAPQVQWRHLQAVSEQAGQVCLADF
                     LNHRSAFYRGGISSPNRAFTAGSRLSPHIALGTLSLRRIFRATAARMDELEAAGTAQA
                     EAWRQGLEGFSHRLYWRDHFVQRLESEPGMEYAPLNAAYAALRYSDNPHMLELWLAGR
                     TGFPLLDACMRCLAARGFINFRMRAMLVNVACFALHLDWRRLQYPLAQRFLDYEPGIH
                     FAQIQMQAGIVGINQMRVYNPTKQLIDQDPKCQFVKRWLPELRRFHPADIAAGIALGD
                     YPPPCVDAAANIAQMKAQITAIRRSPEAAVTTPEVLAKHGSRLRREKAKAKPVAVERQ
                     LVLPF"
     gene            complement(5335..5478)
                     /locus_tag="PKZ52_09695"
     CDS             complement(5335..5478)
                     /locus_tag="PKZ52_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60089.1"
                     /translation="MDFLVLVQLAAVYTAECGIHAAIINPALIVAGLITLESMLSSAA
                     VDE"
     gene            complement(5629..>5992)
                     /locus_tag="PKZ52_09700"
     CDS             complement(5629..>5992)
                     /locus_tag="PKZ52_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60090.1"
                     /translation="LVFIMSLTGCDGRTIQKDDTDFAINGVFINGHTYNSDPLRECTV
                     FYVCTHRLRFNNDGTGLYTSLEKENTFTYKIADGRITTELVGAGDIPQTLQFNVWTHA
                     MELNRVDTGLTFRLHSED"
BASE COUNT         1465 a         1415 c         1613 g         1499 t
ORIGIN      
        1 tgacgagcgt atttggcttg gcctagggcg cggcggagta actcaatttg atgaggcgaa
       61 gggtaaattt acccgcttta actatgtata tggtacgccg ggcgctcccc cttttgccgc
      121 tgccatgtca ctttttgagg acaacaacaa gaatatctgg gtcggacatt accccggcat
      181 ggtgagtttt cacgatcata gttcggaagc tatttccgta taccgcaaaa ataatgaagg
      241 aaataaaggt atttcagata ataatgtttt aggcatagcc gaggattcgc ataaaaattt
      301 atggttggcg ttgggggagg gagttgatta ttttgatcgc cagcatgcct cctttaaata
      361 ctacaacaac aaactaggta attatccggc cagtggcacc ttgtcggctt atttggacag
      421 gcaatcaatt ctttgggtgg gtacctggat ggaggggttt tctcgcttta atcctgctgc
      481 cgatcgcttc gaggtcatgc cgtttaatgc caccctagcc aacgccgatg aacgcagcgg
      541 cccgcagctg cacgattcaa ttgtgtgggg ctattgtgag gataaaaata ataacctgtg
      601 ggtgggtacc cattacgcag gcattaacca ctacgatgca cagcaaaaac aatttactaa
      661 atacaatgct agcgtcggtg gcctggctaa taatatcgcc tggacctgtt ttgaagatag
      721 ccaggggcgg ttttgggtag gcacttcaga tggtttaagc cttaaggata ataaaaccgg
      781 tagttttaaa aattatcggc acgaagaggg taatgccaat agcctgcgct caggttcggt
      841 gttggatatt tacgaggaca gtaaaaaacg cctgtggttt gccaccaatg atgggctgca
      901 tctataccgc gaaaacagcg atgactttga aatttttacc gctgacaatg gttttatcaa
      961 tagtggtatt cgcgctttga ctggggatag gcagggtaat ttatggctgg gcactaataa
     1021 cggtatagtg cgctttaacc ccgatacgct ggatgtaaaa aattatctgt attttgccgg
     1081 caaaaaatgt ggtgctgtta ataccggtgc cgccatgacc agctcggcgg gggaggttat
     1141 ttttggtacc accgatggtt tgattattat cgatgtggaa aagctcacca ctaatcagca
     1201 gcaattacct gtggtattga cggactttaa agtatttgcc aaatcggtaa gtgtggatga
     1261 gccgggctcg ctgcttaaaa aagtgattaa caacacggat aaaattgtgt tggatcatac
     1321 caaaaaaatg gtctcattcg aatttgccct attaaattat cgcagcgcct ataaaaatac
     1381 ctacgcctat atgctggagg ggtttgatcg cggttggcgc gaagtgggcg gcgcccgtga
     1441 ggcccaatac accaatttaa gccccggcac ctacaccttt aaagtgcgcg ccgccaataa
     1501 cgatggcgtt tggacaagcg agcctaaagc catagtgctg gagcaattgc cgccgccctg
     1561 gaaaacctgg tgggcctaca ccttttatgc ggttttaatt gcagtttcta tcgcctattt
     1621 tgtgttcttg caaaagctta agcagcgcca ggtggaagag caaaaccgcc tgctggaatt
     1681 taaagtggct gagcgcaccc gcgatttggc ggaaaaaaac aaggatattc aaaccctgct
     1741 ggccaatatg cgccaaggtt tgttggccat tgaagagggc ggtgtgatcc accatgagta
     1801 ttcagcccac ttagaatcta tttttgaaac caaaaccatc gccgggcaag atgttataag
     1861 ttttctattt aaagatgcat tgttggatga cgatttggtc tgccagatgc gttcgggtat
     1921 taattctatg atcggtgaag atgaaatgaa ttatgtcatg aacgcccata tcctgatccg
     1981 cgaatacccc ttgcgtattg ccgagaaaat caaaattctc tcgttagact ggagccctat
     2041 attagatgcc cagggtaacg tggtgcgttt gatggtatcc atacgcgata gcaccgaatt
     2101 aaaagcctta gagcatgaag cgggggtaaa aagacgtgag ctggaaatta tcggccaatt
     2161 attgcctgta tcggtgaaaa aatttaccga ttattcactt tcggcgcagc gatacctcag
     2221 tgaaagcttg cgcctgctgg aaagtgcagg cgccctggat aacgccctat taaataagct
     2281 gtttcgcaat atacacacca ttaaaggcaa tagccgtatc catggtttta attatattgc
     2341 caatgccgct catgaggcgg aaaccatttt tgaacaggcc cgtagccaca acgccgatca
     2401 ggcgcgccta aaagttacag aggcgattaa acgagttgag gtcgcgctag atgattataa
     2461 ggatatttat cgcactctca tgctgcacaa tcccccttta tcagggcggg gcgatggggt
     2521 gtgggtgagc cgagatggca tcgaggaaat taaaacaaac gctgagcaga ttaaagcagt
     2581 gctaccggaa tccgctcagt cgattttaac cataatcgaa aaatcctctg cagcgccttt
     2641 ggccgatatt attgccgccc ttgttgaatc cctacctgag atggcgcaat ccttaggtaa
     2701 acaaaccccc aaggtggtga ttagtgatgc gggtatttta ataaaagagg cggtccatga
     2761 gcgcctgcgg gcaatattca cccatttatt gcgcaatgcc ttggatcacg gtattgagcc
     2821 tgcagctgtg cgcatggctg cacataaaga cccagctggc accatcagta ttgaggtaaa
     2881 tctggtgaat aatgcgctgt gtatagcact gcgcgatgat gggcgcggcc tggatctaaa
     2941 tcgtttatat cacaagggtt tggagctggg tttggtggat gccacggctg taaacagccc
     3001 cgctaaaatt gccaatagta tttttatccc tggcctcagc actaaacaaa cgctggatga
     3061 tatttccggg cgcggtgtgg gtatggatgc ggtaaaacaa tttttgctgg aaatgggcgg
     3121 tgatattgct gttgaacttt tagatgctga tgtggcgcag gctgagccta aaggagtaag
     3181 ttttgtcttt aaactacgcg tgccgctggc tgtttgtatt gccgaatagt gggaggaacg
     3241 tgtaactgtt ttttgaatct ttttagtgta actgagcgcc gactcaaaac catccggcga
     3301 gtattttaat acagcaaccc ctgcagtgat gaatactggt tattgcaggc tcgccgcgaa
     3361 tacatccctg cataagaatt gttggaatct gcacgcccta aaattcccct ctgttaaggg
     3421 gtgacaggct tgcctgccgg ggtggttgcg gtagggaacc ctgttttagt tcaggggaca
     3481 tggaaatctt ttaccgataa tttcttttat caacaattgt gggcttcgaa caaaaaacag
     3541 ctcagcgatt tagcacccca ctccttcggg gcacccctcc ataggagaga aattttaggc
     3601 cctgcccgtg gtgaaaaagg ccgctcacgc ttactttgag acattacctg ctggccaagg
     3661 cataaatagc aatccgctgc ctgtgcgtat caagcgcttt tgagccaacc caaatgggag
     3721 ccagccggtg gagcgattca ggcgcagcct ttggtggatt aagcgcgata tacgtctgga
     3781 cgataaccca gccctgcaag cagccctcgc ccgcagcgct gagttaatgc ctacatacat
     3841 tatggaaccc agcctgtggc gggcgccgga tgccagcgcc atgcaattag ccgccgtatt
     3901 acaggcactg gcctgcttac agcagagcct gcagatgcgg ggcagtcatc tggtggtgat
     3961 gcagggggag tgcctggtgg cgcttgatca actgcacgcg cactatcctt ttgaagccat
     4021 cttcgcctgt gaagaaaccg gcaataacct tacctaccgg cgcgatttag ccctggcccg
     4081 ctggtgccga atgcgcggta tcgcttttgt ggagctgccc cagtgcggtg tggtgcgcgc
     4141 gggcaatcgc gctagccgca gccaaattgt gcatgagcgc ctatggcaaa ccccgccggt
     4201 tgcggccccc caccacattc taagcccggc agaggggttg ccctttaatc aagctgactg
     4261 gctgcatgac gggcgcaagc agcaggcctg gttgcgcctg tgcccgagcg ccccgcaggt
     4321 gcagtggcgc catctgcagg cggtgtctga acaggccggg caggtctgtt tggcggactt
     4381 tttaaatcat cgcagtgcgt tttatcgcgg cggtatcagc tcccccaacc gggcgtttac
     4441 cgcaggctca cggctctccc cgcacatcgc cctgggcacc ttaagtttgc ggcggatttt
     4501 ccgcgcaacc gccgcccgta tggatgagct ggaggccgca ggcacagccc aggccgaggc
     4561 ctggcggcag ggtttagaag ggttcagcca ccgattgtat tggcgggatc attttgtaca
     4621 gcggttggag tcggagccgg gtatggaata cgcgccgtta aatgccgcct atgctgccct
     4681 gcgttacagc gacaacccgc acatgctgga actttggttg gccgggcgca cggggtttcc
     4741 attattggac gcgtgtatgc gctgcctggc ggcgcggggt tttattaatt ttagaatgcg
     4801 cgccatgctg gtaaacgtcg cctgttttgc cctgcattta gactggcgcc ggttgcaata
     4861 tcccttagcg caacgctttt tagattacga gccgggtatt cattttgcgc aaatacaaat
     4921 gcaagcgggc atcgtgggga ttaatcaaat gcgcgtgtac aaccccacca aacaattaat
     4981 tgatcaagac cctaaatgcc agtttgtaaa acgctggctg ccggaattgc gccgcttcca
     5041 cccggcggat attgccgcag gtattgccct aggggattac ccgccgccct gtgtagatgc
     5101 agcggctaat atcgcccaga tgaaagcgca aatcaccgca attcgccgca gccccgaggc
     5161 agcggtaact acgcctgagg tgctggctaa acatggctcg cgcttgcggc gggaaaaagc
     5221 caaggcgaag cctgtggcgg tggagcggca gttggtgttg ccgttttagg ctggggtaat
     5281 tggcttttag gtacctgttc ccgcgcaggg cgcaggaatg aggacgaaca ccgtttattc
     5341 atccacggcg gccgacgata acattgactc cagtgttatt aacccggcga caattagtgc
     5401 cgggttaata atggcggcat ggatgccgca ctcggcggtg taaaccgccg cgagctgcac
     5461 taaaactaga aaatccacgc attttcgacg gttttagcgc tgcgaaggat aaataaatca
     5521 ggacgatcta tttatccgcc gggttaataa caagtgtatt tttggacgcc tccgatgaaa
     5581 tccccgaccc ttgtatacac gagagctcct cggcggattt gtacacttct aatcttctga
     5641 atgtaagcga aacgtcaatc cagtatccac gcggttaagc tccatggcgt gggtccatac
     5701 attaaattgc agggtttgag gtatatcacc ggcgccaacg agttcggttg taatgcggcc
     5761 gtcggcaatt ttataggtga atgtattttc tttttcgagc gaagtgtata acccggtgcc
     5821 gtcgttatta aagcgtaagc gatgggtgca aacgtagaag acggtgcatt cgcgcagcgg
     5881 gtcggagtta taggtgtggc cgttgataaa tacaccattt atagcaaaat ccgtatcgtc
     5941 tttttgtatg gttctaccgt cgcagccagt gaggctcatt ataaagacga gc
//
LOCUS       DAOQGS010000228         5981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6631_length_5981_cov_4.052481, whole genome shotgun sequence.
ACCESSION   DAOQGS010000228 DAOQGS010000000
VERSION     DAOQGS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_5981_cov_4.052481"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /gene="mltF"
                     /locus_tag="PKZ52_09705"
     CDS             complement(<1..1367)
                     /gene="mltF"
                     /locus_tag="PKZ52_09705"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HNG60091.1"
                     /translation="MSRLLGWLRRLGGLTLIVACSAPLPHSRSLNLLEQIQRAGEIQF
                     VTANGPTTVYEGADGLTGFEYELMHRFADHLGVKTRLVQPGNLQDIVFTLSQNPKAIG
                     AAELVINPGLMRQLDFGPTYVAVASLVVNRIGSAPIESPAQLVGSRLVVRQGSAQEEF
                     LTQLQKSLPELSWQSIQGDPVQLLDRVNEGEADAALVDRLAFEQNQVVFSQVQAAFAL
                     EAEQPIAWAFARNRDKSLQQEVEHFFARLKSDGSLAQLTEQYFTQPSLELDQNLRNFR
                     DLMPKRLAPWRPTLEAAARQYQLDWQVLAAIGYQESQWNAKAVSETGVQGFMMLTNIT
                     AKAMEVNNRLNARDSIFGAARLMRYLLDQQSAFTSPADRLNLALAAYNIGLGHVQDAQ
                     ALAAKAGKNPDAWEAVKPWLAKLDERDTYSKTRHGYARGQGAVLYVDSIATYQELLTL
                     YEQTAS"
     gene            1969..3858
                     /locus_tag="PKZ52_09710"
     CDS             1969..3858
                     /locus_tag="PKZ52_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60092.1"
                     /translation="MSVHDFRTLKRIPFSACSVALLSLAITACSGTGEGSSSSSSSSS
                     SSSSSSSSSSSSSSSSNSSSNSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGSSGY
                     PNIAPYNVVGDASCSGTFACMDFESGSVSTGTLDSTQAYKGSKSVKVTSSAGQFLSLN
                     VPSSNFWVRAFVRSGGSQQYPEQGFARPHGVFLRAVDGQKQMRVGDHRCQLDVNRYGG
                     GWDDLEMISGSYGADNTVCNETVGARMIKNTWYCLEAHYNGPASEVQVFWDNQNVEQL
                     HVKATRIATNADKKPENGYNNQYGNATIPWGPASYTKLEFGYESFNNLGTTPNMTVWY
                     DNVATSSQRIGCGSDYMVKQPLDASTRYPGYAAEEFGASSSSSSSSSSGSTSSSSGGG
                     TAIISETFEAGTSGSAPAGWQSIITHSQLPNTSSSTYSALVDSSRAHSGSKSLRVKVD
                     NTTSSPVFLVKQVPQGVNKLFVRAWVYSSTQLGGVGSSGPGNHGHFMGTMTNVSNNPT
                     NEIRYGIVDGTRLGGMTFIGDAFTFASAGDSTAKVPANTWTCVEYALFDEGGKMYAWI
                     NDKQVLAAENGNAWQNGNGADRVSSMDLAYVHFGWRGGFGSVVKPSDIWFDDIVVSDA
                     RVGCN"
     gene            complement(3920..4405)
                     /locus_tag="PKZ52_09715"
     CDS             complement(3920..4405)
                     /locus_tag="PKZ52_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60093.1"
                     /translation="MKKQIAFLIGAGFICIGYFVWHANNEIKHPSLTTLSDHDRMKLS
                     PPVEQPNEQKSPAIQSNQNKLNYLPESISQKSNPLKKEDVINAMDNRLKNNVQTSPTS
                     HNKPTTEKLNTPPPRRDEDLAFNTPSVVVHTQSRKQEVEKADIPPPPPTHLPLPHGKI
                     Q"
     gene            4483..>5981
                     /locus_tag="PKZ52_09720"
     CDS             4483..>5981
                     /locus_tag="PKZ52_09720"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HNG60094.1"
                     /translation="MFCNIRFFSRAVSWLLVLSAQLSLAESSLRDFDIEVGGRRAVDY
                     ETSQSGEHIDTYTGAIQLTNTDLHIPGNHGMDISIKRVYNSIQPRQFPEPSPYGFGWT
                     ISYGRVIAPDQLSIGKVCSQSQWSVTTKDNPSLEMSHGGRELLVLSRLFNDGSLITAS
                     NWRMICNGGEIKVLSPEGLTYTMGHFVNDQKEPSWYTTKIEDLKGNSLTIKYGGNKNI
                     ESIIRAPEYAKAEFRYSVASGNSDMSHLTHIIGSSNDGAQQVWTYNYDPINDNSLYTN
                     YKLSEVIRPDQTKIKYDYYGLVDNSVNSGALQDPTGSWSIKSVTYPYGGKTEYTYQRV
                     RLHPSFTLYDNPYVAAVKTKTVTDPITNKTGTWTYLFNQRASYGHARDPETGGEVLSY
                     LDLLTVETPAGYPKKLYYHNSAYPYGGRRLNGQSIGKKTMEVTFEVSENYRIEAKTYA
                     DEDGKRIISDENFYLVGTSEYDDFTYAYSEPGEIVSRDKIEFIPLNGEMV"
BASE COUNT         1436 a         1426 c         1572 g         1547 t
ORIGIN      
        1 gaggcggtct gctcgtagag ggtcaacagc tcctggtagg tagcgatgct gtccacgtag
       61 agcaccgccc cttgtccgcg ggcatagccg tggcgggttt tactgtaggt gtcgcgctca
      121 tcgagcttgg caagccaggg tttcaccgcc tcccaggcgt ccgggttttt accggctttg
      181 gccgccagcg cttgggcgtc ctgcacatgg ccaaggccga tgttgtaggc agccagggct
      241 aaattcaaac gatccgcagg tgaggtaaac gccgattgtt gatccagcag ataccgcatc
      301 aggcgggccg cgccaaaaat actgtcgcgg gcattgaggc ggttgttcac ctccatggct
      361 ttggcggtga tgttggtgag catcataaaa ccctgcaccc cagtttccga gacggccttg
      421 gcattccact gggattcttg ataaccaatg gcggccagca cctgccaatc caattgatat
      481 tggcgggccg ccgcttctaa ggttggccgc cagggcgcaa gccgcttggg catcaggtcg
      541 cgaaagttgc gcaggttctg gtccagctcc aggctgggct gggtgaagta ttgctcggtg
      601 agttgcgcca ggctgccatc gcttttgagg cgggcaaaga aatgctccac ttcctgctgc
      661 aggcttttat cgcgattgcg ggcaaaggcc caggcaatgg gctgttccgc ttcgagggca
      721 aaagcagcct gcacctggct gaacaccact tgattttgtt caaaggctaa gcgatccacc
      781 agggcggcat cagcctcgcc ctcgttgacg cgatcgagca gctgtaccgg gtcgccctgg
      841 atgctctgcc agctcagctc aggcaggctt ttttgcagct gggtgagaaa ctcctcctgg
      901 gcggagccct ggcgcaccac cagccggctg cccaccaatt gcgctgggga ttcgatgggg
      961 gctgagccga tgcggttaac cactaggctc gccaccgcca cataggtggg gccaaaatcc
     1021 agttggcgca tcagccccgg attgataacc agctccgctg cacctatggc cttggggttt
     1081 tggctgaggg taaagactat gtcttgcagg ttgcccggtt gcactaaacg cgttttaacg
     1141 cccaggtgat cggcaaagcg gtgcatcaat tcgtattcaa aacccgttaa tccatcggcg
     1201 ccttcgtaca ccgtggtggg gccgttggcg gtgacgaact gtatttctcc agcccgctga
     1261 atttgctcca gcagattcaa gctgcggctg tggggcaacg gggcgctgca ggccacaatt
     1321 aaggtgaggc cgcctaaacg gcgcagccag ccgagcaacc tagacacggc gcaaaacctg
     1381 aggaaaaaaa tctgtgcaca tcatggcgaa tagctcctag ttgattgggg tttgcagcac
     1441 cacagacgct aaaaaacact gggcgcactt ttttaatgca actgacaacc ctatgggcaa
     1501 catttgggcg atggtagaca aggtttttct gagtgcatag cttgtaggcg ggtttaaagt
     1561 gtggtagcca cttgtgcttg cgggaaggaa ttgaagttgt ttcgggtttt tgatgttagt
     1621 ggttgcaaac tatcggctct aggccacccc ttaacttttt ctgcgcgcag gctatttttt
     1681 gcgcatgcag cagaaggcgt aaaaaagccc tcggcgaagg cggattttta ttttgtaata
     1741 tttagatttt gtgacgtaat tctcattgac gcaggggcga ttttaagctg tgggcggaat
     1801 gtgaaccggt gggtagtgta aattttcagc atgccgaaaa atacatattg accaaaaggg
     1861 ttacaagaat gccgctaacc ggcacacccc gcttgggcta gtgatcatcc taaaagactc
     1921 cttattacaa ccataaaact gctgaccctt tttcaccgag agaaccctat gtctgtacat
     1981 gactttcgca ctcttaagcg cattcctttt agtgcttgct ctgtagccct attatccctt
     2041 gccattaccg cgtgttctgg cactggcgaa ggctcttcaa gctccagttc cagctcatca
     2101 agctcaagtt ctagctctag ctcgtcaagt tcctcaagtt cgtcatctaa tagcagctcc
     2161 aattcctcta gcagctcaag ctcatcaagt tcgagttcca gctccagctc atccagcagc
     2221 tcaagctcta gctcctccag ttcaagttcc agcagttcag gctccagcgg ttaccccaat
     2281 atcgcgccct acaatgtcgt aggcgatgcc tcctgtagcg gcacctttgc ctgtatggat
     2341 tttgaatccg gcagcgtaag caccggcacc ctggattcaa cccaggctta taagggctcc
     2401 aagtccgtta aggtcacctc ttccgcaggc caatttttaa gcctgaacgt gcccagcagt
     2461 aacttctggg tgcgcgcgtt tgtgcgcagt ggcggttctc aacaatatcc ggagcagggt
     2521 ttcgctcgcc cgcacggtgt tttcttgcgc gcggtggatg gtcagaagca aatgcgcgtg
     2581 ggtgatcatc gctgtcagct ggatgtcaac cgctacggcg gtggctggga tgaccttgaa
     2641 atgatttctg gttcctatgg tgctgataac actgtatgta atgagacagt cggcgcccgc
     2701 atgatcaaga acacctggta ctgtttagag gctcactaca acggccccgc cagcgaggtg
     2761 caagtattct gggataacca aaacgtcgaa cagctgcacg ttaaggcgac tcgtattgcc
     2821 accaacgccg ataaaaaacc tgaaaatggt tataacaacc aatacggcaa cgccacaatt
     2881 ccctgggggc ccgctagtta caccaagctc gaatttggtt acgaaagttt taacaaccta
     2941 ggtaccaccc cgaatatgac cgtgtggtac gacaacgtag ccacctctag ccagcgtatc
     3001 ggctgcggct cggactatat ggttaaacaa cctctggacg cctcaacccg ctaccccggc
     3061 tacgcggctg aagagttcgg cgccagcagc tcgtcctcct ccagtagctc cagtggttca
     3121 acctcgtcgt catctggcgg cggcacggcc atcatcagcg agacctttga agcgggcacc
     3181 agtggctcag cacccgccgg ttggcagtcc atcatcaccc acagccagct gcccaatacc
     3241 agcagctcaa cctacagtgc attggtggac agcagccggg cgcactcagg ctctaagtcc
     3301 ttgcgggtga aagtagataa cacgacctcg tcaccggtat ttttggttaa gcaagtgccg
     3361 cagggggtta ataaattatt tgtgcgcgct tgggtatata gctcaactca gctgggcgga
     3421 gtgggcagct caggtcccgg taaccacgga cattttatgg gcaccatgac caacgtaagc
     3481 aacaacccga ctaacgaaat acgctacggt attgtcgatg gtacccgttt gggcggtatg
     3541 acatttattg gtgatgcttt tacatttgct tccgctgggg attcaaccgc aaaagtgcct
     3601 gcgaatactt ggacctgtgt ggaatatgcc ttatttgatg agggtggcaa aatgtacgct
     3661 tggattaatg ataagcaagt attggccgca gaaaatggga atgcttggca aaacggcaat
     3721 ggcgcagaca gggttagctc catggactta gcatatgtgc actttggttg gcgcggtggt
     3781 tttggctccg tagttaagcc ttctgatatc tggttcgatg atattgtggt ttcagatgcg
     3841 cgcgtgggct gtaattaatt ttcagcctga gcaataaaaa acccgcgatg aaaatcgcgg
     3901 gtttttttat attcgaccct tattgaattt ttccgtgagg caaaggtagg tgtgttgggg
     3961 gtgggggcgg tatgtctgcc ttttcaactt cttgttttcg agattgggtg tgtaccacaa
     4021 cgcttggcgt gttaaatgcc aagtcttcat ctcgtctggg cggaggggtg ttcaatttct
     4081 ctgtcgttgg tttgttatgt gaggttgggc ttgtttgaac attattctta agcctgttat
     4141 ccatggcgtt aattacatcc tcttttttga ggggattgct tttctggctg atggattctg
     4201 gaaggtagtt taatttgttc tgattggatt ggattgcagg gcttttctgt tcgtttggct
     4261 gttcgacagg tggcgagagt ttcatgcggt catgatcact cagggtagtt aggctggggt
     4321 gtttaatctc attgtttgca tgccagacga aatagccgat acaaatgaat ccagcgccga
     4381 tcaaaaaggc tatttgtttt ttcatgaaat taggttcctt tatttggttc gaatatgata
     4441 gggtgccgcc gaaaaatatt caatggacaa ttggaggagt gtatgttctg caatatcaga
     4501 tttttctcgc gggctgtatc ttggctattg gtgctttcag cacagttgtc tttggcagaa
     4561 tcatcgttaa gagacttcga tatagaagtt ggcggccgaa gagcagtaga ttatgagacc
     4621 agtcaatctg gtgagcatat agatacttac acaggggcga tacagcttac taatactgat
     4681 cttcatatcc cagggaatca cgggatggat atcagcatta aacgagttta taactcgatt
     4741 caacctcggc aatttccaga gccaagcccg tatgggtttg gttggactat ctcgtatggg
     4801 cgagtaatag cgcccgatca gctcagcatt ggaaaagtgt gttcccagtc acagtggagt
     4861 gtcaccacta aagataatcc ctcccttgaa atgtcgcacg gtggtcgtga gcttctcgtg
     4921 ctgagccgct tgtttaatga cggaagtctt atcacagcct ctaactggag aatgatttgt
     4981 aatggcggtg aaataaaagt gctaagccca gaaggtttaa cctataccat ggggcatttt
     5041 gtaaatgatc aaaaagagcc tagctggtac accacaaaga tagaggattt aaaaggaaac
     5101 tcgctgacca ttaaatacgg tggcaacaag aatattgaat ctattatccg cgcgccagag
     5161 tatgctaagg cagaatttcg ttatagtgtc gcgtccggaa atagtgacat gtcgcatttg
     5221 acgcacatta tcggttcatc aaacgatggt gcgcagcagg tatggacata caattatgat
     5281 cctattaatg acaatagtct ttacaccaat tataaactct ccgaagtaat aaggcccgac
     5341 cagaccaaaa ttaaatatga ttactatggt cttgttgata attcagttaa cagtggtgcc
     5401 ctgcaagatc caacagggag ttggtctatt aaatccgtca cctatcctta cggtggtaaa
     5461 acggaatata cctatcagcg cgtaaggctg cacccaagtt ttactttgta tgacaacccc
     5521 tatgttgcag ctgttaaaac gaaaactgta actgatccga ttacaaataa aacgggtaca
     5581 tggacctatt tgtttaatca gcgcgcttct tatgggcacg ctagagaccc agaaactggc
     5641 ggggaagttt tatcttatct ggatcttttg acggttgaaa ctcccgcagg atatccgaaa
     5701 aaactctatt accacaattc agcttatccc tatgggggaa ggaggctaaa tggtcagagt
     5761 attggtaaaa aaacaatgga ggtcaccttt gaggtttccg aaaattatcg catcgaagct
     5821 aaaacctatg cggatgagga tggaaagaga atcatttcgg atgagaattt ttatttggtg
     5881 ggcacaagtg aatatgatga ttttacctac gcatatagcg aaccaggcga aattgtttct
     5941 agagataaaa tcgaattcat tccgctaaac ggggaaatgg t
//
LOCUS       DAOQGS010000229         5975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6645_length_5975_cov_4.332770, whole genome shotgun sequence.
ACCESSION   DAOQGS010000229 DAOQGS010000000
VERSION     DAOQGS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6645_length_5975_cov_4.332770"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PKZ52_09725"
     CDS             complement(<1..1553)
                     /locus_tag="PKZ52_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNG60095.1"
                     /translation="MTGITLLPYDVLFAPQKNPLASNGRYARHLISRCALVCLFMASL
                     SLLAKPAICEEGDEGAAEQASSGGGVPKPPLGEKFFLLSDSAFASTEEARVRIEAPQQ
                     YAISSYGGVDVRLYRIPKPLEFLQKQKNLHRLQTEVNYQGDGIANTLTYLWDSWYKNS
                     RRLMQNVFSAEARTAVINTTPQLGMGEALNKAPTYTYQSQFAPLPNLPLVDEFRYPLT
                     AATTITPPNNVKLDGSSSDFFPPSAGNIYLPLGKRPPGLYLVEAFVHNVRATTLVFVS
                     DTLAITKISGQQLTVWAADKTTGKAVNNSKILWTDGVGVLASGATNNEGLVQLKHNSP
                     ERTYVLGEDEKGGVFIAENFYYDSEIYNTKLYAFTDRPLYRPGDTVNFKILGREFKTA
                     KDHQAAKTLPITLSVIDAAGTTLQTLKTQFDSLQGAQGKFALPANAVAGGYELQFNYD
                     NQLYTSAFRVAEYVKPHFEINLLLAKNQFHTKEAVTGKIQLLYPDGKPVTNAHIELTL
                     RSQQLTMVDA"
     gene            complement(1550..2245)
                     /locus_tag="PKZ52_09730"
     CDS             complement(1550..2245)
                     /locus_tag="PKZ52_09730"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HNG60096.1"
                     /translation="MILTRQLLFILMLPFLFTASQPAWAANSGPAMPSENAAQAVSAP
                     DLTPSQSQLFRRWFITIVDAQLRKGPNPRWQQQDCAGLVRFAANEALKIHDAKWQKNN
                     GFNPVNLPAELIITEEQRLLAQRWHIGQGATGAYARAIILVQHNTELVSRDINQAQPG
                     DLLFFDQGDEQHLMVWMGHFIAYHTGSPTAADNGLRAVTLQQLMHWPDTRWIPDASNP
                     NFSGIYRLGFLSK"
     gene            complement(2242..3903)
                     /locus_tag="PKZ52_09735"
     CDS             complement(2242..3903)
                     /locus_tag="PKZ52_09735"
                     /inference="COORDINATES: protein motif:HMM:NF021432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HNG60097.1"
                     /translation="MEPNGSPARSRLRIVLLTGLGILVLLSGVLVASQGVFGRFWQRA
                     AEPVAIKNRQNLELSAPDGLLEAERLSQLPKDLLSAPFLADLLSDDFVFYYQHNAEKL
                     AVIGTLRRLAFEHNLTLPEQLISQLLDTPAQVALWKGDEGKLSYSLARLELGLAAPLG
                     KVLANFISAASEIAQTDKQLKFAGEISLNKNKVPFFSLDYLSGRSLLFAFQGEHLIVA
                     SHQELLQDEPGHFRPEAQTIIEQLFANQQPFKTHFNLSDTQPKAQARISLDAHFFTLG
                     YQDYLPALEGVRFELSEGKWQSFIGLAKGDHKARFAADLWAKAPADASLCALMPVNPA
                     ALNSIISEQQQNPKITQAFTGRSFVCWFANAPLAKPLIGAELTSDGASAEFDAEITAL
                     FDKTIRNNKVPKTTDEAGNTISPGGYAPSNIQVTDVEGGKQWQKSDGKGKPIATLLRQ
                     HNFMLFSLHAPLLQHGADTLAKRYPALGDKLPNKEAMALFLAPQGLATLLQTEIRQMA
                     ADNDLGPLLPKLTALGKKPALVAQFDAGKQADSAMSWYPLQWNSL"
     gene            4049..4399
                     /locus_tag="PKZ52_09740"
     CDS             4049..4399
                     /locus_tag="PKZ52_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNG60098.1"
                     /translation="MNHERLFATAFSKIYPLYVQKALRKNRSQEEVDQIICWLTGYDL
                     AGLQARIAQGCDLASFFASAPALNSKRSLITGKVCGVQVEAVADPLMQLIRQLDKLID
                     ELAKGRPLAKILRQ"
     gene            complement(4405..5190)
                     /locus_tag="PKZ52_09745"
     CDS             complement(4405..5190)
                     /locus_tag="PKZ52_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNG60099.1"
                     /translation="MPDFLLNALLAGSALALISGPLGSFVVWRRMAYFGDTLAHASLL
                     GVALGLFLQINPWVAVMVGSALLGALLMLLQQQKNLASDTLLGILSHGALAAGLVCVS
                     LMANPQLDLFSFLLGDLLTATRAEALSMAGLSLLLLAVLKLAWRPWLCMALDEDLARA
                     EGLQVDAWQLLFMVMLALVIALAMKVVGVLLITALLIIPAAAARQFCQSPTAMAAGAA
                     LIGVLAVNGGLAASFFWDTPAGPSAVLVAVLVFMLGWLRRRAD"
     gene            complement(5193..5966)
                     /gene="znuC"
                     /locus_tag="PKZ52_09750"
     CDS             complement(5193..5966)
                     /gene="znuC"
                     /locus_tag="PKZ52_09750"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HNG60100.1"
                     /translation="MQAHLLQQVSLQVAKGEVVTLIGPNGAGKTSLVKLVCGLVRPSA
                     GEIVRAADLRIGYVPQRLAFDATLPISVHSFLALTGSQQAAAEAMQRLQIEALKHSLL
                     HSLSGGELQRVLLARALLRRPSLLILDEPAQGVDINGQLELYQTIADLRDELNCGVLM
                     VSHDLHWVMAKTDTVYCLNRHICCQGAPELVSQHPEYLQLFGQNAPAIAIYTHHHDHE
                     HNLHGDVVPQAGHIHGPACNHQHSNPLSKPSAPLNKHRH"
BASE COUNT         1369 a         1518 c         1678 g         1410 t
ORIGIN      
        1 gcatccacca tggttaattg ttggctgcgc agggttaatt caatatgggc attggttacc
       61 ggtttgccat ccgggtagag caattgaatt ttgcccgtca ccgcttcttt ggtgtgaaat
      121 tggtttttgg ctagtaacag attaatttca aaatggggtt tgacgtattc ggctacgcga
      181 aaggcgctgg tgtagagctg gttgtcgtaa ttaaattgca attcataacc gccggccacg
      241 gcattggcgg gcagcgcgaa tttgccctgg gcgccctgta ggctatcaaa ctgggtttta
      301 agagtttgca gtgtggtgcc tgcggcatca ataacggata gcgttatagg cagtgtttta
      361 gcggcttggt gatctttagc ggttttaaat tcccgcccga gtattttgaa attaactgta
      421 tcgccggggc ggtagagggg gcggtcggtg aaggcgtaga gtttggtgtt gtaaatttcg
      481 ctgtcgtaat aaaaattctc agcgataaat acgccgcctt tttcatcctc ccccagcaca
      541 taggtgcgct cagggctgtt gtgttttaac tgcactaaac cctcattatt ggtggcgccg
      601 gaggccagca cacctacgcc atcggtccag agaattttgc tgttattcac cgctttgccg
      661 gtggttttat ccgccgccca cacagttaat tgctggccgg aaattttggt aatggccaaa
      721 gtgtcggaga caaatactaa agtggtggcg cgcacgttat gcacaaaggc ttccaccaaa
      781 tataaacccg gtgggcgctt acccaagggc aaataaatat tgcccgccga aggtgggaaa
      841 aagtcgctgg aggagccatc aagttttaca ttattgggcg gcgtaatggt ggtggctgct
      901 gtcagtggat aacgaaattc atccaccaat ggcaaattgg gcaggggggc aaattggctt
      961 tgataggtat aggtgggggc tttattcagc gcctccccca tgcccaattg cggcgtggta
     1021 ttaataaccg cagtgcgcgc ctcggcggag aaaacatttt gcattaagcg gcgggagttt
     1081 ttgtaccagc tatcccataa ataagtgagt gtattggcga tgccatcgcc ctggtaattc
     1141 acttcggttt gcaagcggtg caggtttttt tgtttctgaa gaaattccag aggtttgggg
     1201 atgcggtaca ggcgcacatc cacaccgcca taactgctga tggcgtattg ttgcggcgct
     1261 tcaatgcgca cgcgggcttc ttctgtgctg gcaaaggcgc tgtcggataa taaaaagaat
     1321 ttttccccca agggaggttt tggcacgccg ccaccgctac tggcctgctc agcggcgccc
     1381 tcgtcacctt cttcgcaaat tgctggcttt gcaagcaggc ttaagcttgc cataaataaa
     1441 cacactagcg cgcagcggct aattaaatgg cgggcgtaac ggccattgct tgccaaggga
     1501 tttttttggg gcgcgaataa aacatcatag ggaagaagag ttatgccagt catttgctta
     1561 aaaatcctag tcgataaatt ccagaaaaat tcgggttgct ggcatcgggt atccagcggg
     1621 tgtcgggcca atgcattaat tgttgcagcg ttactgcccg caggccgtta tcggcggcgg
     1681 tggggctacc ggtgtggtag gcaataaaat gccccatcca caccattagg tgttgctcat
     1741 caccctggtc aaaaaataat aaatcgccgg gctgggcttg attaatatcc cggctcacca
     1801 actcggtatt gtgctgcact aaaataatgg cccgcgcata ggcgccggtg gcgccctggc
     1861 ctatgtgcca gcgctgggct aataaacgct gctcttcagt aataattaat tccgctggca
     1921 aattaaccgg gttaaaacca ttattttttt gccatttggc atcgtgaatt ttcagcgctt
     1981 cattggcggc aaagcgcacg aggccagcgc agtcctgttg ttgccagcgg gggtttggcc
     2041 ctttgcgcag ttgtgcatcc acaattgtga taaaccagcg gcgaaataat tgcgattggc
     2101 tgggggttaa atccggggcg gatactgcct gcgctgcatt ttcgctgggc attgcggggc
     2161 cagaattggc cgcccaggca ggctggctgg ccgtaaataa aaacggcaac attaaaatga
     2221 ataataattg ccgggttaat atcacaggct attccactgc agcgggtacc agctcatggc
     2281 agaatccgct tgtttgcccg catcaaattg cgccaccagg gcgggttttt tacctaacgc
     2341 ggtgagtttg ggcaacagcg ggccgaggtc attatccgct gccatttggc gaatttctgt
     2401 ttgcaataaa gtggctaggc cctggggggc taaaaataac gccattgcct ctttattcgg
     2461 taatttatcg cccagggccg gatagcgttt tgccagtgtg tcggcgccgt gttgcagcaa
     2521 aggtgcatgc agggaaaaca acataaaatt gtgttggcgc aaaagggtgg caatgggctt
     2581 tcccttaccg tcgctttttt gccattgttt gccgccttct acatccgtca cttggatatt
     2641 agacggcgca taaccacctg gggaaattgt attgccggct tcgtcggtgg ttttaggcac
     2701 tttattgttg cggatggttt tatcaaacag cgcggtaatt tctgcatcaa attctgcgct
     2761 tgcgccatcg ctagttaatt ccgcacctat aagcggtttg gctaagggag cgttggcaaa
     2821 ccaacacaca aaactgcggc ctgtaaaggc ttgggtgatt ttagggtttt gttgttgctc
     2881 gctaattatg ctgtttagcg ctgcggggtt taccggcatt agcgcacaca ggctggcatc
     2941 ggccggtgct tttgcccaga ggtctgcagc aaaacgcgcc ttatgatccc ctttggctaa
     3001 gccgataaag ctctgccatt tgccttcact taattcaaag cgcacgccct ccagagcggg
     3061 aagataatcc tgataaccga gggtaaaaaa atgtgcatct aaactgatgc gagcttgtgc
     3121 tttgggctgt gtatcgctga gattaaaatg agttttaaac ggttgttggt tggcaaataa
     3181 ctgctcaatg atcgtctgcg cttccgggcg aaaatggccc ggttcatcct gcagcagttc
     3241 ctggtggctt gccactatta aatgttcacc ttgaaaagca aacaataagc tgcggccgga
     3301 taaataatcc aggctaaaaa acggcacttt atttttatta aggcttattt cgccggcaaa
     3361 ttttaattgt ttatccgttt gggcaatttc acttgctgcg ctgataaaat ttgccagcac
     3421 cttgcctaaa ggcgcggcca ggcccaactc taaacgcgcc agggaatagc tgagtttacc
     3481 ctcgtcccct ttccacaggg ccacctgggc gggggtgtct aacagctggc taattaattg
     3541 ttcgggaagc gttaaattgt gttcaaaggc taagcgccgc aatgtgccga taactgcgag
     3601 tttctcggca ttgtgctggt aataaaaaac aaaatcgtcg cttaacagat ccgccaaaaa
     3661 aggcgcgctt aacagatctt tgggtaattg gcttaaacgc tcggcttcca gcagcccatc
     3721 cggagcagaa agttcgaggt tttggcggtt tttaatcgcc accggctccg ccgcccgctg
     3781 ccaaaagcgc ccaaaaaccc cctggcttgc caccagcacc ccgcttaaca gcaccaaaat
     3841 acctagcccg gtcaataaca caatgcgtag gcgtgagcgg gcgggcgagc cgttgggttc
     3901 catggggtgc tcctgtttaa gcggaatttt aaggcggcta tgctaacaag ttttaccgtc
     3961 ttaggagtgg ggttttgcag accgcaccga ttgcacgggc ggttatgccc actttgttaa
     4021 ctaacatttt ttaagcagga aaaagcctat gaaccacgag cgcctttttg ccacggcatt
     4081 tagcaagatt tatcccctct atgtgcaaaa agccctgcgc aaaaaccgca gccaagaaga
     4141 ggtggatcaa attatttgtt ggttaacggg ctatgatctg gcgggcctgc aggcgcgcat
     4201 tgcgcagggc tgtgacttgg cgagtttctt cgccagcgcc cccgccctta actccaagcg
     4261 cagtttgatc acgggcaagg tttgcggtgt gcaggtggaa gcagtggccg accctttgat
     4321 gcagctgatc cgccagctgg ataagctgat cgatgaattg gccaagggca ggcccctggc
     4381 aaaaatcctg cggcagtagt gggtttagtc tgctcgccgc cgcagccaac ccagcataaa
     4441 aaccagcaca gccactaaca cggcggaagg gcccgccggt gtatcccaaa aaaacgaagc
     4501 cgccaggccg ccgttgaccg ccagcacgcc gatcaacgcc gcgcccgccg ccatggcggt
     4561 ggggctttgg caaaactggc gggcagcggc ggcagggata atcaacagcg cagtaatcag
     4621 caatacgccc actaccttca tggccagggc gatcaccagg gccagcatca ccataaacaa
     4681 caattgccag gcatccacct gcaggccctc ggcgcgggcg aggtcttcat ccagggccat
     4741 gcacagccag gggcgccagg cgagcttaag cacagccagc agcagcaggc tcagccccgc
     4801 catgctgagg gcttctgcgc gggtggcggt gagcagatcg ccgagcaaaa aactaaataa
     4861 atccaactgc gggttggcca ttaggcttac gcagaccaaa cctgccgcca gggcgccgtg
     4921 ggagagaatg cccagcagcg tatcgctggc aaggtttttt tgctgttgca agagcattaa
     4981 gagtgcgccc aataaagcgc tgcccaccat cacagccacc caggggttga tctgtaaaaa
     5041 cagccccaag gccacgccca ataggctggc atgggcgagg gtatcgccaa aataggccat
     5101 gcgccgccag accacaaaac tgcccaaagg gccgctgata agggcaaggg cggagcctgc
     5161 cagtaacgca tttaataaaa agtcgggcat attcagtggc gatgtttatt taggggggct
     5221 gagggtttgc taagcgggtt gctgtgctga tggttgcagg cggggccgtg gatatggccg
     5281 gcctggggta ccacatcgcc gtgtaggttg tgttcgtgat cgtggtggtg ggtatagatg
     5341 gcgatggcag gggcattctg gccgaacagc tgcaggtatt ccgggtgctg gctgaccaat
     5401 tccggcgcgc cttggcagca gatatggcgg ttgaggcaat agaccgtatc ggttttcgcc
     5461 atcacccaat gcaggtcgtg ggacaccatc aacacgccgc aattaagttc atcgcgcagg
     5521 tcggcaatgg tttgatacag ctccagctgg ccgttgatgt ctacgccctg ggccggttca
     5581 tcaagaatca gaagcgaagg ccgccgcaat aaagcgcgcg ccaacaacac ccgctgtaac
     5641 tccccgccgg agagtgaatg cagcaggctg tgttttagtg cctcaatttg cagccgctgc
     5701 atggcctcgg cggccgcctg ttggctgccg gttagcgcca ggaagctatg cacactgatg
     5761 ggcagcgtgg catcgaaggc caggcgctgg ggcacataac cgatgcgcaa atccgccgcc
     5821 cgcacaatct cccctgcgga tggccgcacc aggccacaca ccagtttcac cagggaggtt
     5881 ttgcccgcgc cgttagggcc gatcagggtg actacctcgc ccttagccac ctgcaggctc
     5941 acctgctgca gcaggtgagc ctgcaggtgg ctaag
//
LOCUS       DAOQGS010000230         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6647_length_5974_cov_4.936307, whole genome shotgun sequence.
ACCESSION   DAOQGS010000230 DAOQGS010000000
VERSION     DAOQGS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6647_length_5974_cov_4.936307"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PKZ52_09755"
     CDS             <1..685
                     /locus_tag="PKZ52_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNG60101.1"
                     /translation="LTDGLDAVLENITQLATTPTGPLRITLPKFVYDWKLKNIFAQFC
                     RAHPAINLEISINDATIDLIEHNFDIGIRFGDKLDPRMVARPISHSIRDAIFASPTYL
                     KRTGTPKTPADLTGHRYIQYRFHASNKRLAFSVDNYPAPLPADLSVALVVNDTYAIID
                     AALEGLGIGRVLEPVVERFITQGDLVPILEPYWPICPPIYLYYPQTLQQSGVVKAFVD
                     FFIAANQLP"
     gene            complement(1235..2632)
                     /locus_tag="PKZ52_09760"
     CDS             complement(1235..2632)
                     /locus_tag="PKZ52_09760"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNG60102.1"
                     /translation="MTSRNEAQTKLKQRSLIATSLLGGSLLLTACGGNGHKPTPTVDL
                     TAVRVIHASADAPKVKIAVDTGLAEGGKNYNYLENTAIVSRNAGPVDLVVKAQLPDGT
                     SADVITAKGVNLAADKEYEVFAVGSVANKTLEPFILARPDKFNTSNVRVSVAHLTEAA
                     PLVDVYVTAPGASLAASAKLGSFSYKETLGPVEIPAGNYQIRVTASGSTTALYDSGTL
                     SLKAGEDWVAAAVPNVVPKGAPNKSPIGLYLLNDQSQLFVPSANDGASLRVVHNSADA
                     PAVDIVVNNQFASPLVKNLSFPNFTGYVTVPAATYNVKVAAAGTQTTVINADLPLAKG
                     ANYTVIALDTLAKISPLVLADKPRSIATEAQLRAIHGSSLAGLVDVYVTAPGASINGV
                     NAAIKNFAFKADSGYLGLPPGKYAISLTAPGTKNIALGPLDVTLEAGGIYTVLARDEV
                     GLGGASVTLLDDFNP"
     gene            complement(2805..3395)
                     /locus_tag="PKZ52_09765"
     CDS             complement(2805..3395)
                     /locus_tag="PKZ52_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HNG60103.1"
                     /translation="MLMTTASKHRWPLIRRSSGRNFGFAALCCAGVLLCLSGCARING
                     EDYVHNSPAFNLQTFFTGPVKAWGLVQDRSGRVIQQFDIAMQGSWQGNQGTLVEDFSF
                     YDGKKQQRIWHITAKGDGSFEGRADDILDKARGLSFGNALQWTYVMDVPVKDGSYRMH
                     FDDWMWAMHDGVLINRSYMKKFGITFAEITIFMQKQ"
     gene            complement(3462..4724)
                     /locus_tag="PKZ52_09770"
     CDS             complement(3462..4724)
                     /locus_tag="PKZ52_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60104.1"
                     /translation="MVSSFSQRLRHGLLALPLTFAGLPIYIHAPDYYAVELGIPLATI
                     GFLLLVLRIIDAVQDPLIGALSDRFYPHRQAIVVSGALLLAGGFWLIFHPPQLISHSA
                     QLIWLAVSVFICTTGFSMVSINLQAAGSLWLMPAQERTLVAATREALGLLGLLLAAVV
                     PAVLFNYLPRPAALHNLTLILVPLLCLGVWVFCHWLRGATLLTPAAQGSMSWRDLFAF
                     KWINGFLLVYTLSAFAAAIPGILVLFYVRDYLQLERYSGVFLLLYFLSGVLAMPLWQA
                     LTTKISKPAAWLWSMVLACSAFSAAFYLPPQALIAFSLVCIGSGMALGADLALPQAIM
                     ADHIAAQPEAASRYSALMAFLSKAALALATGIVLPIVGWFGFQPGASAHNEVLPFAYA
                     LVPCCIKALCAVFLFYCSRRYYLAPAAA"
     gene            complement(4724..5230)
                     /locus_tag="PKZ52_09775"
     CDS             complement(4724..5230)
                     /locus_tag="PKZ52_09775"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNG60105.1"
                     /translation="MRIFALCLFAAAPAFGLPQAVRTELNNPAALGSGRFSFLFSDVY
                     HISLYTPEQAFNPAGAFALKIDYLMPLKGAAIIERTIELIKRQAFADQTKLMQWQNTL
                     AGFFPDVDKGVSLTGFKDKQGHTQFYRNEQWIGAVADPEFSHYFFAIWLADDTSEPKL
                     RRALLGLR"
     gene            complement(5527..>5974)
                     /locus_tag="PKZ52_09780"
     CDS             complement(5527..>5974)
                     /locus_tag="PKZ52_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNG60106.1"
                     /translation="PVQVNSASLALTTFTNHNFNAFSHQVFSSSDDSWTEASINGVNR
                     PADSSFTLLSATLIDGNSQTYTWDVLAGVVGTDGLGGSNNLLTLVVRPDLSQNNSSFG
                     PHFWDRTAPIGAPKLTLDISPVPEPASWALLLAGGAFILRRKRRTH"
BASE COUNT         1600 a         1545 c         1451 g         1378 t
ORIGIN      
        1 cctcaccgat ggcctggatg cggtactcga gaatattacc caattggcca ccacccccac
       61 cggccccctg cgcattacac tgcctaaatt tgtttacgac tggaaattaa aaaatatctt
      121 tgcgcagttc tgccgcgcgc acccggctat caatctggag atttccatta atgacgccac
      181 cattgattta attgagcata attttgatat tggcattcgc tttggcgata aattagatcc
      241 gcgcatggtg gcaaggccta tttcccacag cattcgcgac gccatttttg ccagccccac
      301 ctacttaaaa cgcaccggca cccccaaaac gcccgccgat ttaaccggcc accgctatat
      361 tcaataccgc tttcacgcct ccaataaacg cctagcattt tccgtagata attaccccgc
      421 ccccctgcca gcggatttat ccgtagccct agtggtaaat gatacctacg cgattattga
      481 tgctgcatta gaagggcttg gcataggccg agtgctggag cccgtagtag agcgttttat
      541 tacccagggg gatttggtgc caattttgga gccctattgg cctatctgcc cgccgattta
      601 tttgtattat ccgcaaaccc tacaacaatc tggcgtggta aaagcttttg tggatttttt
      661 tatagcggct aatcaactgc cgtaaatgac acgggcataa aagcggttaa aaatacatga
      721 ccggcaatgc cctttttatt gatatcaacg cctttttatt gatgctggag aagcctctcg
      781 cacagattag caaaatcagg ctagcgcttt aatgctacaa acctttgcag cgctgaacac
      841 ttacaaaaat acccggcaag tctacttgtc gggtattttt gtagtgtaac tattcagcac
      901 actttactcc cttcttccgc gcgggcggca aattcaggag ggtttagcca tccggcataa
      961 tcgattcgcg gtggtgcagt ggtgactaca gattagtgca gaccgtcaaa aagagggacc
     1021 tttttgacga gcctacacgg atgtattcac ggcgtgtctg cactaacctg tagtcaccac
     1081 tgcgggcgct ggattacggg gtcacagcac taaaacacac acgcgttagt tcccagtcag
     1141 tgcaggaatt acctccagcc acacaaaaag atgctgaata gttacgttgt agttagcttt
     1201 gcggctcgtt tatgcaaata agccgcactc ttaattaagg attgaaatca tccaaaagag
     1261 tcacacttgc gccacccaaa cccacttcat ccctcgccag cacagtataa ataccaccgg
     1321 cttccaaggt tacatccaag gggcctaaag caatattttt agtgccaggg gcggtgaggc
     1381 taatggcgta tttaccgggc ggcaggccta aataaccgct gtccgcttta aaggcgaaat
     1441 tctttatagc ggcatttacg ccattaatgg acgcaccggg agcagtcaca tatacgtcca
     1501 ccaaacctgc taaagaggaa ccgtgaatag cgcgcagctg cgcttcggtg gcgatggagc
     1561 ggggtttatc agctaatact aaaggggata ttttcgcaag tgtatccaga gctattaccg
     1621 tgtaattagc acccttggcc aggggtaaat cggcattaat taccgtggtt tgagtaccgg
     1681 cagcagccac ttttacattg taggtagccg caggcacagt cacataaccg gtaaaattag
     1741 ggaagcttag gtttttaacc aatgggctgg caaattgatt atttactaca atatccaccg
     1801 ccggagcatc ggcgctgttg tgcaccaccc gtaaacttgc gccgtcgttg gcgctgggca
     1861 caaataattg actttgatca tttaataaat acaagccaat gggggattta ttgggcgcgc
     1921 ccttaggaac cacattaggc accgccgctg ccacccaatc ctcacccgct tttaaactca
     1981 aggtgccgga atcataaagg gcggtggtgg agcctgaggc ggttacgcga atttgataat
     2041 taccagcggg aatttccacc ggccccaagg tttctttata ggaaaaactg ccgagtttgg
     2101 cgctggcggc aaggcttgca ccgggggcgg taacgtatac atccaccaag ggcgcagctt
     2161 cagttaaatg ggcaactgaa acccgcacat tgctggtatt aaatttatcc gggcgggcga
     2221 gaataaacgg ctccaatgtt ttatttgcca cgctgcctac agcaaatact tcatattctt
     2281 tatcagccgc caaattaacg cccttagcgg ttatgacatc ggctgaggtg ccatcgggca
     2341 actgggcttt taccaccaaa tccacaggcc ctgcattgcg gctgacgatg gcagtatttt
     2401 ctagatagtt ataatttttg ccgccttcag caaggcctgt atccacggcg atttttactt
     2461 taggcgcatc ggcgctggcg tggataacac gcacggcggt taaatccact gttggagtgg
     2521 gtttgtggcc attgccgcca caagcagtta ataaaaggct gccacctaaa aggctagtgg
     2581 cgattaggct gcgttgtttt agttttgttt gggcttcgtt acgactagtc atgataggct
     2641 ccgaatgggt ttggatagtg ccaagccttt tacgcaggca cgcccatgcg gatgagcaga
     2701 agattctatt ttgcgtgcac tcactcagaa atcgaaaaaa taatgcgctt aataaatcca
     2761 aactcgcaca ccggcgtaag aggcctttta attgttcagt ggccctactg cttctgcata
     2821 aaaatagtaa tttcagcaaa ggtgataccg aattttttca tgtaggagcg attgattaat
     2881 acaccgtcgt gcatggccca catccaatca tcaaagtgca ttctgtagga accatctttt
     2941 accggcacat ccattacata ggtccactgc agggcgttgc caaaacttaa accccgcgcc
     3001 ttatccaaaa tatcgtctgc gcgcccttca aaactgccat cgcccttggc cgtgatatgc
     3061 cagatgcgct gctgtttttt gccatcgtaa aaactgaaat cctccaccaa agtaccctga
     3121 ttgccctgcc aactgccctg catggctata tcaaattgtt gaatgaccct gcccgagcga
     3181 tcctgcacca agccccaggc tttaactggc ccggtaaaaa atgtttgcaa attaaacgcg
     3241 ggggaattgt gcacataatc ctcgccgtta atgcgcgcgc agccggataa acacagcagc
     3301 acaccggcac agcacagtgc ggcaaagcca aaattgcgcc cactggatct gcggatcaga
     3361 ggccaccggt gtttagaagc agtagtcatc aacatatcat tgtgcctttt taaataaact
     3421 caggtaattt tgcccgtgcc gcagattaat attcgattta tttacgcagc agcaggcgct
     3481 aaataatagc ggcggctgca atagaataaa aaaacggcgc aaagggcttt aatgcaacag
     3541 ggcaccagtg cgtatgcaaa gggcaatacc tcattgtgcg cgctggcacc cggctgaaat
     3601 ccaaaccaac cgacaatggg taatacgata ccggtggcca aggccaaagc ggctttggat
     3661 aaaaatgcca ttaatgcaga atagcggctg gcagcctccg gctgtgcagc gatatggtcg
     3721 gccattatgg cttgcggcag ggctaaatca gcgcccaatg ccatacccga gccgatacac
     3781 accaggctaa aagcgattaa cgcctggggc ggtaaataaa atgctgcact aaatgcgctg
     3841 caggcgagta ccatgctcca cagccaggca gcgggtttgc ttatttttgt cgtgagcgcc
     3901 tgccacaggg gcatagctaa tacaccactt aaaaaataca gcagtaagaa cacgccactg
     3961 tagcgctcta actgcaaata atcgcgcaca taaaataaaa ctaaaatgcc ggggatggcg
     4021 gcggcaaatg cgcttagggt atacaccagt aaaaaaccat taatccactt aaaggcaaat
     4081 aaatcccgcc agctcatgga gccctgggca gcgggtgtta aaagtgtggc gcctcgcaac
     4141 caatggcaaa atacccacac ccccaggcac agcagcggca ctaaaataag cgttaaatta
     4201 tgtagcgcag ccgggcgcgg caaatagtta aataatacag cgggcaccac ggcggccaat
     4261 aaaagcccga gcaggcctaa ggcctcacgg gtggcagcaa ctaaggtgcg ctcctgggct
     4321 ggcatcagcc aaaggctgcc agctgcctgc agattaatac tcaccatgct aaaacccgtg
     4381 gtgcaaataa atacactcac cgccaaccaa attaattgtg cgctgtggct tatcaactgc
     4441 ggcgggtgaa atatcagcca aaaaccaccg gccagtaaca gcgcaccgct tacaacaatg
     4501 gcttgtcggt gagggtaaaa gcggtcactc aaggcgccga tgagcggatc ttgcaccgca
     4561 tcgataatgc gcaataccaa taataaaaag ccgatagtgg caagtgggat acccagctca
     4621 accgcgtaat aatccggtgc gtgaatataa atgggcaggc ctgcaaaggt taagggcagc
     4681 gccaataacc cgtggcgcag ccgctgggaa aagctgctca ccattagcgc aggcctaaca
     4741 atgcgcggcg caatttaggt tcgctggtgt catcggccag ccaaatagca aaaaaataat
     4801 ggctaaattc cggatctgcc accgcgccta tccactgctc attgcgataa aactgcgtgt
     4861 ggccctgttt atctttaaac cccgtaaggc ttacgccctt atccacatcg gggaaaaatc
     4921 cagccagagt attttgccat tgcattaatt ttgtttggtc tgcaaatgcc tggcgcttta
     4981 tcagttcaat cgtgcgctca ataatagcgg cgccttttaa cggcattaaa taatcaattt
     5041 ttaacgcgaa ggcgccagcg gggttaaagg cctgctccgg ggtgtacaag ctgatgtggt
     5101 atacatcgct gaataaaaaa ctaaagcggc cgctgcctag ggccgcaggg ttattaagct
     5161 cagtgcgcac cgcctgtggc aggccaaaag ctggcgctgc ggcgaataaa cacagagcaa
     5221 aaatacgcat tatttttatc actgtgtcac cttcttaaac ggcaagggtc attcggcaag
     5281 tggcgacgct ctggcaaggt agtatttcaa caagctatta agccgagcca ggcaaaaaat
     5341 tatgctcgta gcttaaaaaa agtggccaaa tgcagtctta atgaatacgc cccgcatggg
     5401 ctgctggatg aatctagcgc tttgaggaaa tattcctagc caagaaaact gccgaataaa
     5461 aagcgcgaag ccttgacccg gcggcgctgc gccgggtcat ttggattttt gcctgctacc
     5521 gggcaattag tgagtacggc gcttgcgccg cagaataaaa gcgccacccg ccaacagcag
     5581 cgcccagctg gcaggctccg gcacggggct gatatccagc gttaatttag gcgcgccgat
     5641 tggcgcggtg cgatcccaaa aatgggggcc aaatgaactg ttgttttggc ttaaatcagg
     5701 ccgcaccacc agagtgagca gattattgct gccgcccaag ccatcggtgc ccaccacccc
     5761 cgccagcaca tcccaggtat aggtttgcga attgccatca attaaggtgg cggataaaag
     5821 cgtaaagctg ctatcggccg gacggttcac accgttaata ctggcctctg tccagctgtc
     5881 atcgctggag ctaaatacct gatgggaaaa tgcgttgaag ttgtggttgg taaacgtggt
     5941 gagcgccagg ctcgcgctgt tgacctgcac tggc
//
LOCUS       DAOQGS010000231         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6698_length_5950_cov_4.451230, whole genome shotgun sequence.
ACCESSION   DAOQGS010000231 DAOQGS010000000
VERSION     DAOQGS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_5950_cov_4.451230"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1539
                     /locus_tag="PKZ52_09785"
     CDS             292..1539
                     /locus_tag="PKZ52_09785"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malectin"
                     /protein_id="HNG60107.1"
                     /translation="MVKLNTALRGGRLFGGLMVLVATALSGCGGQPSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSGIPSNEKLVVAVNSGGAALTVGDVTYVADQY
                     FSGGTANSTTEAISGATDSALYQTERYGTFSYDIPVTAGTYTIKLQFAEIYQTSAGKR
                     AFNVAIEGKQQLSNVDLYTLAGKNGAYNYEVKNIRVTDGKLTINLEGTVDNATIAGFS
                     VYSADGKLDTSTPPPPPPGGADTMGFIGCSMAENTATGYRAIGGKKMWAPYGTNGDVV
                     QNWTSNTSAAWTKFDNQVAKFGKPTEVWIMICIFAQNGVTYDEVKKLIANARSHAAAG
                     AKIYITGQPLYASGLTCDLAGAGGPEKTDSLAKQAAADTSLNVVYPGAFGPMTSGDRT
                     DSCHANTTGQQKLGQQAAGFWGK"
     gene            complement(1811..3103)
                     /gene="phoR"
                     /locus_tag="PKZ52_09790"
     CDS             complement(1811..3103)
                     /gene="phoR"
                     /locus_tag="PKZ52_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNG60108.1"
                     /translation="MPSEGYRTELVWLLGFIGAVGFLGYFFEQTLLALLIATSLYCAR
                     IVYQTHRLARWVYHARRSTAADDSLASIWSDIAEDVRFLQLRHEREKQRLQAVVVRVQ
                     EMTTALTDGVILIDKRDNVEWWNQAAGHLFGFKEVDQGHKLTNIIRTPKFVHYFDRRD
                     YQQPLELNSFRKEDQQLQVQVHPFGQGERLVVARDITRLFKLEQMRKDFIANLSHELR
                     TPLTVIKGYLETLADSEQMPKNWRQPFSQMSQQASRMNGLIQDLITLSKLETQEREPQ
                     RKPVELGPLLASVVDDAKALSTAHGHSFIVQGDLNARLLGNSDELRSAFANLVVNAVN
                     YSPDGGKIIVSVQRSAGGLEVSVKDNGVGIDPKHIPRLTERFYRVDPSRSVLTGGTGL
                     GLAIVKHILLPHDAQLRINSMPGKGSTFYCEFPPEAVA"
     gene            complement(3112..3819)
                     /gene="phoB"
                     /locus_tag="PKZ52_09795"
     CDS             complement(3112..3819)
                     /gene="phoB"
                     /locus_tag="PKZ52_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNG60109.1"
                     /translation="MSLSKTILIVDDEPAIRDMLRVALEMANYEVLEAADAKAAHGLI
                     VDNKPDLILLDWMMPGTSGMELARRLKRDELTAAIPIIMLTAKVEEDNKIQGLDAGAD
                     DYITKPFSPRELVARLKAVLRRTDTLTSGDPIKAGELVLDPVSHRVSIQGAPVQMGPT
                     EYRLLEFFLTHQERVYSRSQLLDHVWGGNVYVEERTVDVHIRRLRKAIGIADHDQFIQ
                     TVRGAGYRFSLKHLSSE"
     gene            complement(3873..4760)
                     /gene="ubiA"
                     /locus_tag="PKZ52_09800"
     CDS             complement(3873..4760)
                     /gene="ubiA"
                     /locus_tag="PKZ52_09800"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNG60110.1"
                     /translation="MVSSLTPAARLYAYARLMRLDKPIGFFLLLWPVLWSLWLAAKGA
                     PSLANLLIFSLGALVMRSAGCVINDYADRHIDGQVSRTRLRPIVLGHVSSTEALGLFV
                     GLCLLALLLVSLTNRLTLYFSVGALVLATAYPFMKRYTYLPQVVLGAAFAWSIPMAFA
                     AETGSVPPQAWLVYTAVVMWTTAYDTFYGMVDREEDIKIGVKSTAILFAEQDRAMTGL
                     LQGFFLLTLWVVGHKMGLGWPYYLGLLAAAVLSAYQQWLIRHREPAACFRAFLHNNWV
                     GMAIFLGLVAQFALVIKPQ"
     gene            complement(4825..>5950)
                     /locus_tag="PKZ52_09805"
     CDS             complement(4825..>5950)
                     /locus_tag="PKZ52_09805"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNG60111.1"
                     /translation="VVARRMRKYLRQDDCVARLGGDEFIILLRDVADKQAVCRVVELL
                     IEKISSPIRLDVAMVHVGMSAGVAVYPFDGDTVQTLMRNADSALYDAKGRGRGCFQVY
                     RPELTESANLRLMLDADLRTAINNEEFEIYYQPLIDIDSGKVSSVEALLRWHHPKKGL
                     LGPEDFIPYALESGLICHMGDWVIVSACEQLARWRDEGIALAMSINVSARQFQTDALV
                     ARVARCLQQFNLDPQQLIIEITEQIFMEEVDAVLQRVQALRNLGVKISLDDFGTGYSS
                     LNYIVRFSPDYLKIDRSFVARIGEQAEHNGMVEAIVGLGRIVPLGIVAEGVETQEQFD
                     FLKTCGCHFAQGFYFSKPCCAEDLSHYLLHQQPVAQAQCH"
BASE COUNT         1456 a         1663 c         1559 g         1272 t
ORIGIN      
        1 aaacatctgt atttccttaa ttaataagcg gcctactcag gcgttaaatt cgcgataaat
       61 cagccgtatt ttcacaatcc ttggggttta aaaaatgcgg ttttacaggt ggtgtaattg
      121 ctatctccag cggcagaata attttaaccc agccaggttg gatcaacgtt ttctcactca
      181 cgctggattt gagtgtttag ccctctgcac gcaatggcat ttgcagggtt cttacacaac
      241 tgcgcctgcc aaaaacttcc tcacaataaa attaagtcaa ggtgcatttt tatggtaaaa
      301 ctcaatacag cgctgcgtgg cggcaggtta ttcggtggat taatggttct ggtcgcaaca
      361 gccctcagcg gctgcggtgg gcaaccgtcc tcctcgtcca gttctagctc aagttctagt
      421 tccagctcaa gctcctccag tagttcgtct tcctcatcga gctcttcaag cagttcaagc
      481 tcttcctcaa gctcatcaag tagcggtatc cccagcaacg aaaaactcgt cgtcgcggtt
      541 aactccggtg gcgcagcatt gactgttggc gatgttactt atgttgccga tcagtatttc
      601 tccggcggca ccgccaatag cacaacagag gccatctccg gcgcaacgga ttctgcgctg
      661 taccaaaccg agcgctatgg cactttctcg tacgatattc cggtaactgc tggcacctat
      721 acaattaaat tgcaatttgc cgagatttac caaacttcag cgggtaagcg tgcatttaat
      781 gttgccattg aaggcaaaca acaattatcc aatgtcgatt tatacaccct agcgggtaaa
      841 aacggtgcct ataactacga ggtgaaaaat atccgcgtta ccgacggcaa attgacgatt
      901 aatttggaag gcacggtaga taacgccact atcgcaggat ttagcgtgta ttcagccgat
      961 ggcaaattag acacctcaac acctccgcct ccaccccccg gcggcgcaga caccatgggt
     1021 tttatcggct gttccatggc ggaaaataca gccacaggct atcgcgccat tggcggtaaa
     1081 aaaatgtggg cgccttatgg caccaatggt gatgtagtgc aaaactggac ctctaacacc
     1141 tcagccgcct ggacaaagtt tgataaccaa gtggccaaat tcggtaaacc taccgaagtg
     1201 tggatcatga tttgtatctt tgcgcaaaac ggcgtgacct acgatgaagt gaaaaaactc
     1261 atcgccaatg cgcgctccca tgccgctgcc ggggccaaaa tttatatcac tggccagccg
     1321 ttgtacgctt cgggtttaac ttgtgactta gccggtgcag gtggccctga aaaaaccgat
     1381 agcctggcga aacaggcggc agcggatacc agtttaaatg ttgtttatcc tggcgcgttt
     1441 ggccctatga ccagcggcga tcgcacagat agctgccatg ccaatacaac gggccaacag
     1501 aaattgggcc aacaggcggc tgggttctgg ggtaaataag ctagctcagt aatgcccctg
     1561 agggggcatt tccattattt aaaactccag caccaaaaga tttccgaatt cgcaagccgt
     1621 cggaggggcg ctccttgtag gtgaccctac cggctaaacc cgcaaggatt gtctggcgcg
     1681 ctttgggcaa ccacaggggt agcccctgcg aatggcatcg ctcgctgaag gcaacgaaga
     1741 cgctgtgcat ttagttttga ttatccacac ctcagaatgg cctgaggatg cagatataaa
     1801 cccatcacgc ctaagccacc gcctccggcg gaaattcaca ataaaacgta ctgcccttac
     1861 cgggcatgga gttgatacgc agctgggcgt cgtggggcag gaggatgtgt ttgactatgg
     1921 caaggccgag gcccgtgccg ccggtgagca ccgagcggct cggatccacc cggtaaaagc
     1981 gctcggtgag gcggggtatg tgtttggggt ctatacccac gccgttgtcc ttcacggaaa
     2041 cctccagccc accggcagag cgctgtaccg agacaataat cttgccgcca tccggcgagt
     2101 agttcaccgc gtttaccacc aggttggcaa aggcgctgcg cagctcgtcg ctgttgccaa
     2161 gtaagcgagc gttgagatcg ccctgcacaa taaagctgtg gccatgggcg gtactaagcg
     2221 ctttggcatc gtccaccacg ctggccagca aggggcctaa ttccaccggt ttacgctggg
     2281 gctcgcgctc ttgggtttcc agtttggaga gggtgattag gtcttgaatc agcccgttca
     2341 tgcggctggc ctgctggctc atctgactga acggctgccg ccagtttttg ggcatttgct
     2401 cgctgtcggc gagggtttct aaataaccct tgatcaccgt aagcggggtg cgcagttcat
     2461 gggagagatt ggcgataaaa tctttgcgca tctgttccag cttaaacagg cgggtaatgt
     2521 cccgcgccac taccaaacgc tcaccctggc caaaagggtg tacctgcact tgcagctgct
     2581 ggtcctcctt gcggaaggag ttaagctcca aaggctgctg gtagtcccgg cggtcgaagt
     2641 agtgcacgaa cttgggtgtg cggatgatgt tggtgagctt gtggccctgg tccacctctt
     2701 taaaaccaaa taagtgcccg gccgcttggt tccaccactc cacgttgtcc cgtttgtcga
     2761 tgaggatcac cccatcggta agggccgtag tcatctcctg cacccgcact accaccgctt
     2821 gtaagcgctg tttttcccgc tcgtggcgca gctgtaaaaa gcgcacatcc tcggctatat
     2881 cgctccagat actggcgagg ctatcatcgg ccgccgtgct gcggcgggcg tgatagaccc
     2941 agcgggccag gcggtgggtt tgatagacga tgcgggcgca atacagggag gtggcaatca
     3001 acagggccag cagggtttgc tcaaaaaagt aacccaaaaa acccaccgca ccgataaaac
     3061 ccaacaacca cactagttca gtgcgataac cttcagatgg catagagcaa cttattcgct
     3121 tgagaggtgc ttaagggaga agcggtaacc cgccccgcgc acagtttgga taaactgatc
     3181 gtgatcggca ataccaatag ccttacgtaa acgacggata tggacatcca ccgtgcgctc
     3241 ctccacatac acattgccgc cccagacgtg gtccagcagt tggctgcggc tgtacacccg
     3301 ctcttggtgg gtgaggaaaa actccagtaa acggtattcg gtgggcccca tttgcaccgg
     3361 tgcaccctga atactcaccc ggtggctgac cgggtctaac accagctcgc cagctttgat
     3421 ggggtcgccg ctggtgaggg tatcggtgcg gcgcagaacc gccttcaggc gcgccaccag
     3481 ctcccggggg gagaagggtt tggtgatgta atcatcggca ccggcatcca ggccctggat
     3541 tttattgtct tcctccacct tggcggtgag catgatgatg ggaatggcgg cggtgagttc
     3601 gtcgcgcttt aagcgccggg ccagctccat accggaggtg ccgggcatca tccagtccag
     3661 caggattaaa tccggtttgt tatccacaat caggccgtgg gcggctttgg cgtcggcggc
     3721 ttctagaacc tcgtaattgg ccatctccaa tgccacccgc agcatgtcgc gaatagctgg
     3781 ctcatcgtcc acaatcaaaa tcgttttact caggctcata cgcgcgcaac taacttaggg
     3841 gggtgaagga atgttgcgat tagataaacc ttttattgcg gttttatgac aagggcaaat
     3901 tgagccacta agcctaagaa gatcgccata cccacccaat tgttatgcaa aaaagcgcga
     3961 aagcaggcgg cgggctcccg gtggcggatc aaccactgct ggtaggccga cagaaccgcc
     4021 gccgccagca ggcctaaata atagggccag cccaagccca ttttatggcc caccacccac
     4081 agggtgagca ggaaaaaccc ctgcagcagg ccggtcatgg cgcgatcctg ctcggcaaat
     4141 aaaattgccg tggatttaac gccgattttt atatcctcct cgcgatccac catgccgtaa
     4201 aaggtatcgt aggcggtggt ccacatcacc acagcggtat acaccagcca ggcctggggc
     4261 ggcacgctgc cggtttcggc agcaaaggcc atggggatcg accaggcaaa ggccgccccc
     4321 aataccacct ggggcaaata ggtgtagcgc ttcataaacg gataggcggt ggccaaaacc
     4381 aaagcaccga cggagaaata aagtgtgagc cggttagtaa ggctcaccaa cagcagcgcc
     4441 agcaggcata ggcccacaaa caggcccaaa gcctcggtac ttgaaacatg ccccagcaca
     4501 ataggccgca ggcgcgtgcg gctaacctgg ccatcgatat ggcgatcagc gtaatcgttg
     4561 atcacacagc cagccgagcg cattactaaa gcgcccaggc taaaaatcag cagattggcc
     4621 aatgagggcg cccccttagc cgccagccac aaactccaca acaccggcca cagcagtagg
     4681 aaaaagccga tgggtttatc cagccgcatt aaacgggcat aggcgtagag cctggcggcg
     4741 ggggtgaggg atgacaccat gaggctacct taataacaaa agtgcccatg gtattggttt
     4801 agctgtgtga agcaaagggc gcggctagtg gcactgggcc tgcgccaccg gctgttgatg
     4861 cagcaagtaa tgggataaat cctcggcaca acaaggcttg gaaaaataaa aaccctgcgc
     4921 aaaatggcag ccgcaggttt tgagaaaatc aaactgctcc tgggtctcca ccccttcggc
     4981 cactatgccc aagggcacta tgcggcctag gcccactatg gcctccacca tgccgttgtg
     5041 ctcggcctgc tcaccaatgc gggccacaaa cgagcgatca atttttaaat aatccggcga
     5101 gaagcgcaca atataattaa gcgatgaata accggtgcca aaatcatcca gagaaatttt
     5161 aacccctaaa ttgcgcaggg cctgcactcg ctgcaacacc gcgtccacct cctccataaa
     5221 tatttgttcg gtaatttcaa taattaattg ctgcgggtct aaattaaact gctgcaggca
     5281 acgggccacg cgggcaacca gcgcgtcggt ttgaaattgc cgggcggaga cattaatgct
     5341 catggcaagg gcaatgccct catcgcgcca gcgggctaat tgttcgcagg cgctaacgat
     5401 gacccaatcc cccatatggc aaatcaggcc gctctccaag gcgtaaggaa taaaatcctc
     5461 cggccctaac aggccttttt tcgggtgatg ccagcgcagt agcgcttcca ctgaagaaac
     5521 tttaccgctg tcaatatcaa ttaatggctg gtaataaatt tcgaattctt cgttattaat
     5581 agcggtgcgt aaatccgcat ccagcattaa gcgcaaatta gcgctttccg tcagctctgg
     5641 ccgatacact tgaaaacaac cgcggccgcg ccccttggca tcgtacaggg cggaatcggc
     5701 gttgcgcatg agggtttgca ccgtatcgcc atcaaacggg tacacggcaa ctcccgcgct
     5761 catgcccaca tgcaccatgg ccacatccag gcgaatcggc gagctgattt tttcaattaa
     5821 taattccacc acccggcaca ccgcctgttt atcggccaca tcgcgcagca aaataataaa
     5881 ttcatcgcca cctaaacgcg ccacacaatc atcctggcgc aagtacttgc gcatacgccg
     5941 cgccaccacc
//
LOCUS       DAOQGS010000232         5921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6751_length_5921_cov_4.597170, whole genome shotgun sequence.
ACCESSION   DAOQGS010000232 DAOQGS010000000
VERSION     DAOQGS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6751_length_5921_cov_4.597170"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /gene="gltB"
                     /locus_tag="PKZ52_09810"
     CDS             complement(<1..1291)
                     /gene="gltB"
                     /locus_tag="PKZ52_09810"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488724.1"
                     /note="catalyzes the formation of glutamate from glutamine
                     and alpha-ketoglutarate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNG60112.1"
                     /translation="MSTGLYRLDEFKDNCGFGLIAHLKGKPSHKLLATGIEALTCMTH
                     RGGIAADGKTGDGCGLLIQKPDSFLRAVAQEQGFALAPLYAVGSIMLSRDPVLANSAR
                     SVVSEELAAVGLPQLTWRQLPTDPSCLGPIALESLPDFYHLLVNAQGESDAQFESKLF
                     IARRKIEKRLAGDSAFYVASLSSQVLSYKGLMMPVDLPQFFSDLADPRLETAICVFHQ
                     RFSTNTLPRWPLAQPFRLLAHNGEINTIVGNRNWSQARTNKFQSELLPELASISPLVN
                     RTGSDSSSLDNMLEVLLTGGMELHRAIRMLVPPAWQNMETMDQDLKAFYEFNSMHIEP
                     WDGPAGLVITDGRYAVCTLDRNGLRPSRWVITNDDIITVASEVGVYSYAPEDVVAKGR
                     LGPGQIMSVDTQTGQLMHTEDIDNQLKSTQPYLKWLKE"
     gene            complement(1514..3163)
                     /locus_tag="PKZ52_09815"
     CDS             complement(1514..3163)
                     /locus_tag="PKZ52_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG60113.1"
                     /translation="MQTPPVSLELEPYIPTSWRQLQRQIENLAQFGGAVQVITGAEGA
                     GKSTFLEDLLQQASTQQGWEIWQVGDEAWPGQALQKLLEALGLPVVAEQSVGESMVAL
                     RKYNQNLQRQKRRVVVAVDNADLLSDATLSGVVSVLQGGAEEGFGLHLVMFARESLAD
                     RLDALQLIDVSVYDFVLPCLSLQELTELLNGEYQDLSTRESVRPLSADALHDIWLESQ
                     GLPGVALTLAREAWALSMGDEKRFSLRGLPLVHILLVVVLISVLVWAIWMREKPSETA
                     PTITQETTLQSINNKPLTIEMKQVNPGESSEPVKLPSAAPEAVQSPQQALNNYTATQA
                     APTESAPVPQPAAPVTPPVEEAKPEAVKTVAAKPEVAVKPEPVKEAKPEPSKDTKPAS
                     KPADAKPAEAPKAAGASDGIKPVKPVEEPAKAGDSKTLVKADEKPKMKQEKTADAPVG
                     IAASSKKLSKMDENAFVLQIMVTNSAKKVEDILAGAPNKKDLAAYKTIRDGKEVFIVV
                     EGEYPSANAAAAAAKNLPSMQQNAKPWPKKVGLIHAEIKSP"
     gene            complement(3659..4702)
                     /locus_tag="PKZ52_09820"
     CDS             complement(3659..4702)
                     /locus_tag="PKZ52_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60114.1"
                     /translation="MENKIILAVKDFANSINSGSKIVANMSALMDVIDKLPLSSFDYW
                     ERLIRSEFSLALIESNTPTRSLWSTPKEMITWLDLISWDGYRREKALRTLSGAAPNTF
                     FFSLAVRRLNDWVPQVREAARERLPEIARSTDPKYVVEALCVALSNWNSWGRVEEVDK
                     SILLQMICEKEIANSLRCKLISSACGPMPSLFSQLGRTPILDGNIEEIANFAIQPSVR
                     AKAYRSLFEGRIVWIEGRKWEWTDIRYCVGRLKPIFAERKLDIKTPLIDLLKLSSDDP
                     SSIVRRVSAEILIRELGKLDSVARILAEKFASDKSYPVSERGEFALKKLDEADHASAL
                     KPKVGSLSNPPSV"
     gene            complement(4844..5704)
                     /locus_tag="PKZ52_09825"
     CDS             complement(4844..5704)
                     /locus_tag="PKZ52_09825"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNG60115.1"
                     /translation="MVNDGKNYEQFVKSLQQALLDSEDLIGQKNIQVETNKKLMDSCG
                     VEREFDVYWEYELAGITYKTVIECKDYKSSISVEKIDALIGKIRDLPDIKPIFATRTG
                     YQSGAEIKAKANKIDLLIVRQQSEEDWQDQEGNPYIKEVAINMELVSPARIISFSAEI
                     DRGWVKENTDIDTSKSFGLDARNGMITIEDVEKGEKYTVHQLGYDLGAVHQGESGEFI
                     KTNGFINAFIYIDGLRLKLRSYTVRYSVSLPIIIPINIDYSKELLGVVEYLHRGTKTA
                     VFANKIIKDW"
BASE COUNT         1425 a         1547 c         1406 g         1543 t
ORIGIN      
        1 gctctttcag ccactttagg tagggctgag tggatttaag ctggttgtcg atgtcctcgg
       61 tgtgcatcaa ctggccggtt tgggtatcca cagacatgat ctggccgggc cctaagcggc
      121 ctttggcgac cacgtcttcc ggcgcgtagc tgtacacgcc cacttcggag gccacggtga
      181 tgatgtcgtc attggtaatg acccaacgag acgggcgcaa accgttgcga tcgagggtac
      241 acaccgcata gcggccgtca gtaattacca agccagccgg gccatcccaa ggctcgatgt
      301 gcatggagtt aaattcatag aaggctttta agtcctgatc catagtctcc atgttctgcc
      361 aggccggtgg caccagcata cgaatggcgc ggtgcagctc cataccaccg gttaacagca
      421 cttccagcat gttatcaagg ctcgatgagt cggagccggt gcggttgacc aggggcgaga
      481 tggacgcaag ctcaggcaat aactcactct ggaatttatt ggtgcgggcc tgggaccagt
      541 tgcggttgcc aacgatggtg ttgatctcac cgttgtgggc cagcaaacgg aagggctgcg
      601 ccaggggcca gcggggcaaa gtgttagtag agaagcgctg gtggaaaaca cagatggcgg
      661 tttctaaacg cgggtctgcc aggtctgaaa agaactgggg caggtccacc ggcatcatca
      721 ggcctttata agacaacact tggctgctaa ggctggctac ataaaaggcg ctgtcgccag
      781 ctaagcgttt ttcaatcttg cggcgggcaa taaataattt ggattcaaac tgcgcatcgc
      841 tctcgccttg ggcgtttacc aataaatggt agaagtccgg caggctttcc aaggcgatgg
      901 ggcctaggca gctggggtcg gtgggcagct ggcgccaggt gagttggggc agaccaacgg
      961 cggccagctc ctcgctgacg acgctgcgcg cgctgttggc caatactggg tcgcggctga
     1021 gcataataga gcccactgca tagaggggcg ccagggcaaa gccctgctcc tgggccacag
     1081 cgcgcaggaa gctatcgggt ttttgaatca acaggccgca gccgtcgccg gttttaccgt
     1141 ctgcggcgat cccacctcgg tgggtcatgc aggttagggc ttcgatgccc gtagctagga
     1201 gtttatgact gggcttgccc tttaagtggg caatcaaacc aaaaccacag ttatctttaa
     1261 actcatctaa ccggtaaaga ccagtactca taggctgttt cacttcgcga tagagggtta
     1321 gcgctgctcg accggccaaa ataaaccttt tcaaaacaaa aggttaagaa gaggctgggc
     1381 gacagacaga caaaaaagcc cctttgaaag gggcttgcct aataaatggg cgcagaatgt
     1441 taactgctcg ttcagttatt ggcaagttgc gcctttggcg cgcaaacctg ccaatgtcag
     1501 acaggttctt aacttagggc gactttatct cagcatgaat caggccgact tttttcggcc
     1561 agggtttagc gttttgttgc atgctgggta agttcttcgc ggcggcagca gcggcattgg
     1621 ccgatgggta ctcgccctcc accacaataa agacttcttt accgtcgcgg atggttttat
     1681 acgccgccag atcctttttg ttgggtgcgc cagccaaaat atcttccacc tttttggccg
     1741 aattggtcac catgatctgc agcacaaaag cgttttcatc cattttgcta agctttttgc
     1801 tgctagcggc gatacccact ggggcatcgg cggttttttc ctgcttcatt ttgggctttt
     1861 catcagcttt taccagcgtt ttgctatcac cggccttggc aggctcctca acgggcttca
     1921 ccggtttgat cccatcactg gcccctgcgg ccttcggggc ctccgcaggt tttgcgtcgg
     1981 cgggtttact ggccggttta gtgtctttgc tgggttcggg tttagcttct tttaccggct
     2041 cgggttttac agccacctcc ggcttggccg caaccgtctt caccgcctcg ggtttcgcct
     2101 cttccaccgg tggggttacc ggtgcggcgg gctggggcac cggcgcgctc tcagtggggg
     2161 ccgcttgggt ggcggtgtaa ttattcagcg cctgttgggg cgactgcacc gcctcggggg
     2221 cggcgctggg caatttgacc ggctcggagg actcgccggg gttaacctgc ttcatctcaa
     2281 tggtgagggg tttgttattg atggactgca gggtggtttc ctgggtaatg gtgggggcgg
     2341 tttcgctggg tttttcccgc atccagatgg cccacaccaa cacactaatt aacaccacca
     2401 ccaaaagaat atgcaccaag ggtaaacccc gcagggaaaa ccgtttttca tcgcccatag
     2461 ataaagccca tgcctctcgt gccagggtaa gcgccacacc gggcagcccc tggctttcta
     2521 accaaatgtc gtgcagcgcg tccgccgata gggggcgcac cgattctcgg gtactcaaat
     2581 cctgatattc accgtttaac agctcggtaa gctcctgcaa actcaaacag ggcagcacaa
     2641 agtcgtacac gctaacgtcg atcagctgca gcgcatcgag ccggtcggcc aggctctcgc
     2701 gggcgaacat caccaggtgc aggccaaagc cctcctccgc cccgccctgc aatacactca
     2761 caacgccgga taaggtagcg tcgctgagca ggtcggcatt atccaccgcc accaccaccc
     2821 ggcgcttttg gcgctgcagg ttttggttgt acttgcgcag cgccaccatg gactccccca
     2881 cggactgctc cgccaccacc ggcagcccca aagcctccag cagtttttgc agcgcctggc
     2941 ccggccaagc ctcatccccc acctgccaaa tctcccaacc ctgctgggtg ctggcctgct
     3001 gcaacagatc ctctaaaaaa gtgcttttac ccgccccttc cgccccggta atcacctgca
     3061 cggcgccgcc gaactgggcc aggttttcaa tctgccgctg caactggcgc cagctggtgg
     3121 ggatataggg ttctaattca agcgaaacgg gcggagtctg catggcgggt gagcattcgg
     3181 ggcttgatta aaggtgcgac aatgtagcgt cttataacag gcaaagcaaa accctaagcc
     3241 ttagggattc ctgtgggggc gagcttagcg gtgtaggctt gcagctgtgt gacaggggtc
     3301 ttaataatgt ttgggtagtg cttgtgcggg tgggttgttt gatgttgtcg ggctgtagat
     3361 ttaaataggt atacgctcaa ctgcaacttg ggcgcgcaat aataattttg ggggcccaga
     3421 aatagctacg attcctaaat agtgctctaa atgcagctta agcacttgct cttctatttt
     3481 taagttccag caacgctgat cgatactagc gatttttttc ctgccatacg ccagtcaatt
     3541 cacatttgag agttgctgta atttttaaca tgaattttgg taggggggat tcggagcgaa
     3601 gcgacaatcc accatttctc tgccgtaata tcgtcgttca aaataataat tcttgccctt
     3661 aaaccgaagg tgggttgctt agcgaaccca ccttcggttt aagggcactg gcatgatccg
     3721 cctcgtccaa tttttttaat gcaaactcac cccgctcaga tacagggtaa gatttatctg
     3781 aagcaaactt ctcggctagt attctagcta cagaatcaag tttcccaagc tctcgtatta
     3841 aaatctccgc tgaaactcgc ctaacgattg atgaaggatc atcagatgaa agtttcaaca
     3901 aatcaattag tggagtttta atatctaatt ttcgctcagc aaagataggc ttcaaccttc
     3961 ctacacaata ccgaatgtcg gtccactccc atttccggcc ttcaatccat acgattcgcc
     4021 cttcaaataa gctacggtat gctttagcac gaacagaagg ctgaatagca aaattagcaa
     4081 tttcctcaat atttccatca agtattggcg tgcgaccaag ctgagaaaag agtgaaggca
     4141 taggcccgca agccgatgag attaacttgc agcgaagcga atttgctatt tccttctcac
     4201 aaatcatttg cagaagaata ctcttatcaa cttcttcgac cctaccccaa gaattccaat
     4261 tggaaagtgc aacgcataga gcttcaacca catactttgg atcagtagat cttgctatct
     4321 cgggtagcct ctcccttgca gcctcacgaa cttgaggcac ccagtcatta agcctgcgga
     4381 cagcaagaga aaaaaagaat gtatttggtg cggcaccaga aagtgttcgt agagcttttt
     4441 cccgtctata gccatcccag ctaattagat caagccaagt tatcatttcc ttaggcgttg
     4501 accataggct ccgcgtcggg gtatttgatt ctattaatgc caaagaaaac tctgaacgaa
     4561 ttaagcgctc ccaataatca aagctcgaaa gaggtaattt atctatcaca tccattagag
     4621 cagacatatt agccacaatt ttgcttcccg aatttatcga gttagcaaag tcttttactg
     4681 ccaggataat tttgttttcc ataatctgcg acttttgcac ctgccactca cctcttaacg
     4741 ccgttgtaaa atgcagtctt ggaggagccc gcttgtgcta ttcagcacaa aaaggagaga
     4801 cggaaaaatt ccaatttaac aacattgcta tattcgtttc ttactaccaa tccttaataa
     4861 ttttattagc aaaaactgcc gttttagtac ctctatgcag atactcgact actccgagca
     4921 actctttgga gtaatcaata ttaattggaa ttattatagg taacgaaacc gaatacctaa
     4981 ctgtatacga tctcaatttc aacctcagac catcaatata aatgaatgca tttataaaac
     5041 cattcgtttt aataaattcg cctgattcac cctgatgaac cgcacccaaa tcatatccca
     5101 attgatgcac cgtgtatttc tcgccttttt cgacatcctc aatagtgatc atgccatttc
     5161 tagcatctag accgaaagac ttactcgtat caatatcagt attctcttta acccagccac
     5221 gatcgatctc agcagaaaaa ctgataattc tcgccggaga aaccaattcc atgttaatag
     5281 caacctcttt tatgtaaggg tttccctctt gatcctgcca gtcttcttcg gattgttggc
     5341 gcactatcaa caaatcaatt ttgttagcct tggcttttat ttctgcaccc gactgatagc
     5401 cggttcttgt tgcaaagatc ggtttaatat caggtaagtc tctaatctta ccaatcagtg
     5461 cgtcaatttt ttcaaccgaa atagatgact tataatcctt gcattcaata acagttttat
     5521 aagtaattcc agcgagttca tactcccaat agacgtcaaa ttctcgctca actccgcagc
     5581 tatccatcaa cttcttattt gtttcaactt gaatattttt ctgcccaatt aaatcttcag
     5641 aatcgagcaa tgcttgctga agacttttta caaattgctc gtaatttttt ccgtcgttta
     5701 ccacgatacc tccttagcac tcaaagaata taacgacaat ttttgccggt cgcatagcgg
     5761 atcggcaaaa acgccttgtt aaatgccgat ctcatcttat ggcaccatgg ttgcgaagat
     5821 atgaaatgac cttacctaac agctcttcat tatctgacct cacctttagc cttccatgtc
     5881 taaccggatc aatatctacc ctctgcgact catcacttcg t
//
LOCUS       DAOQGS010000233         5914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6765_length_5914_cov_3.229732, whole genome shotgun sequence.
ACCESSION   DAOQGS010000233 DAOQGS010000000
VERSION     DAOQGS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_5914_cov_3.229732"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4002
                     /locus_tag="PKZ52_09830"
     CDS             <1..4002
                     /locus_tag="PKZ52_09830"
                     /inference="COORDINATES: protein motif:HMM:NF039490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60116.1"
                     /translation="GEIVGSQPIARFERISDFTHLPLAQNFSATLTAANQPNQWVVTD
                     NKVLLNGLSYSGFTQLIGHEGADDFVLSGKQTLTIDGKNGSDSLTLSSAQGTVVGWQL
                     TGANGAITAAAAGNTELITPLFSNIEQLIGGAGDDVFVLTATSNRYSRFDGGTGANSL
                     ASSTANNQWQLGLLSSDAHEPVDHLNNAEVVRIQTLHGSGSDSLLGSDDNALWHVGRG
                     GLVSLSQTNATDKRSLEATGMDRVLGGRGQDDFILQDIGSASASFARLDGGSVGADSV
                     TGVNTSSVRDRLDARAVSGDVQVNLNEVPVSTSLTANQLVLSGIETLQLNDATNNTLY
                     GPTLPSKQAVSWQITQAKQGSLAPKQGAHGENTVSFSGVSNLIAGAGEDRFALATPEG
                     LGGDLDGGDGTDYLDYSQATLAASQSGFDVVIGDNTRLNNIEGVIGKNTGSMRLVVKS
                     NGSQWLLDDIDGDSTTSDGMNDGRVTLANGKSVDFVDFAALVGTQGVDGFEIKGGFNG
                     SITTGEGTDTVLVSVNPGRTQTIKLINDASDRLSLIWQGSAPAGVKETYKQAATGRES
                     IALESLNPALSLQPFSIDFSALASINDRLTVEEMRVQTAFESAGRNRLSLHPNALQLA
                     IDSPSGADPDLIGANPNTSEANPDSGAKIGTWFNVENKTNLILSGQAGDQLNLSGSSQ
                     WAGTLSLEKFAITAQGEQSLAAGQINFNSVASIGSAKTAVQIATPLVQLNNLRGDAWV
                     ASSGDIKLASLDGQGRLNLRADGVVGQTQALSYTRDLTLAAGGAINLDNPDNRLTGGL
                     SLSAANTITLANGLTSISALNAEHFTYTGQDLTVAGPMVVAGNTDVTSTGSIDALNPA
                     NHLARLRVNTAGTAAFALGADSTLVGAKVGGALHVQADGLTVAGDLTAASLDLIAGQP
                     GTGAGQLKVSGQLNAPQISLSAGDIVLKTQLQGIDLSLNAKSVSLEKAIDLQGNFSAQ
                     VDSLQQAAAADISAKGAFTLNASGALNLDGAITSGRDISIAAGGTIQQSGAWRTEGTG
                     ASGQTKPSTADVVVKSGGNITQHANANTQSAGGITYEAAGAVSLAALGARREVVVNAT
                     GGIIDANGTSTNITGPSARLISGGDISGLDLSVDSLVGQFAGGAKTINLRNDKTLRID
                     RLIAGSAAEATVTTISTTRGDVIFNNDASKNPEVANLYSKPDLAREKTGVANSNFSTG
                     TLNILSEKGRVKATGSVNINQPDLVANNVNIFSALGVSTPGRPLVIYSAGKVLYTGTG
                     LNWKPFTAFGQRFEFTSDNALDLSQLLLAAGDQLIEIEPLEDVNPAVFTDIRVYTSGT
                     NAILLPGDQRYEE"
     gene            4171..5049
                     /locus_tag="PKZ52_09835"
     CDS             4171..5049
                     /locus_tag="PKZ52_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60117.1"
                     /translation="MIKVAIHASVGSKGKNMEKDIKTVTALINVYRRSLNQTSIVMSA
                     KTSTELEGAIGEFQSNHLKSKSPDSKVDAGGKTFAALKLFYQTFFKAIALTPPTYGEV
                     TWNSEGAEGGKYHSRCFTVPSSSSGLTIGRGYDMKEKSQAKIASDLTSIGLAASQSDT
                     IKKAAGLNGAPAEYFVVENDLLDFQLTPAQQLALFKISYDEQAAEVKRICGLDKNVKD
                     YGQVDWIKLDSSIKDIAIDLKFRGDYTPASRKLIQKAIADNDLAEFKKQLKNAVNWPN
                     VPKDRFDRRVKFLDEH"
     gene            5156..5485
                     /locus_tag="PKZ52_09840"
     CDS             5156..5485
                     /locus_tag="PKZ52_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60118.1"
                     /translation="MQTKRAKSVLIRVIQYNTDTAALRLESIKPIEHVLLDSVDVSAF
                     TVDGRKYEFSKCDELSVNSVAVEAEQVSAEFECFVPKATAIVAKCSIPIRADKFDAMS
                     CVKAARE"
     gene            complement(5541..>5914)
                     /locus_tag="PKZ52_09845"
     CDS             complement(5541..>5914)
                     /locus_tag="PKZ52_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNG60119.1"
                     /translation="PAGFWTIGYGHLCTPNHPAITELEAEQYLAQDLSTALNATLRLC
                     PVLIQEPEHRLAAIVDFTFNLGAGRLQTSTLRLRINQKNWVEAARELKRWVHGGGKML
                     PGLVLRRDLEVRHLLGAESLN"
BASE COUNT         1444 a         1529 c         1661 g         1280 t
ORIGIN      
        1 ggtgagattg tgggcagcca gccaattgct cgcttcgagc gcatcagcga tttcacacac
       61 ctgccgctgg cccagaattt cagcgccacc ctcactgcgg ccaaccagcc taaccaatgg
      121 gtggtgaccg acaataaggt gctgctcaac ggcctgagtt attcgggctt tacccagctg
      181 atcggccatg agggcgccga tgattttgtc ctcagtggca agcaaaccct caccatcgat
      241 ggcaaaaatg gcagcgacag cctgacccta agctcagcgc aaggcactgt agtgggctgg
      301 cagttgactg gtgccaatgg agccatcacg gcagctgccg ccgggaatac cgagcttatc
      361 accccgctgt ttagcaatat tgaacagcta atcggcggtg cgggcgatga cgtgtttgtg
      421 ctcactgcca ctagcaaccg ctacagtcgt tttgacggtg gtaccggcgc caatagcctg
      481 gccagcagca cagccaataa ccaatggcaa ttgggcctgt tatcgagcga cgcccacgag
      541 ccggtggatc acctaaataa cgctgaagtg gtgcgcatac agaccctaca cggctctggc
      601 agcgacagcc tgctcggtag cgacgataac gctttgtggc acgtgggccg aggtggcctt
      661 gtgagcctaa gccaaaccaa cgccaccgat aaacgcagcc tggaagccac tggcatggat
      721 cgcgtgctcg gcggtagggg ccaagatgat tttattctgc aggacattgg cagcgccagc
      781 gccagctttg ctcgcctcga tggcggttct gtgggcgccg acagtgttac cggggtgaat
      841 accagcagtg tgcgcgaccg tttggatgcg cgggcggtga gtggcgatgt gcaggtgaat
      901 ctcaatgagg tgcctgtcag taccagccta actgccaacc agctggtgct gtctggcatt
      961 gaaacccttc agctcaacga cgctaccaat aacaccctgt atggccctac gctgcccagc
     1021 aaacaggccg tcagttggca aattacccag gccaagcagg gcagcttagc gcctaaacag
     1081 ggtgcccatg gcgagaatac ggtgagtttt agcggtgtga gcaacctgat tgccggtgcg
     1141 ggtgaagacc gctttgcgct tgccaccccg gagggcctgg gcggtgactt agacggtggc
     1201 gatggcactg actacctcga ctacagccaa gcgaccctgg cggctagtca aagcggcttt
     1261 gacgtggtga tcggcgataa taccaggttg aacaatatcg aaggtgtgat cggcaaaaac
     1321 actggcagca tgcgtctggt agtcaagagc aatggctccc agtggctatt ggacgacatc
     1381 gatggcgaca gcactaccag tgatggtatg aacgatggcc gggtcactct ggccaatggt
     1441 aagagcgtgg attttgtgga ctttgctgct ttggtgggca cccagggtgt ggatggattt
     1501 gagatcaaag gtggctttaa cggcagcatt accaccggcg aggggaccga tacggtgttg
     1561 gtgagtgtca accccggtcg cacccaaacc atcaaactca tcaacgacgc tagcgatagg
     1621 ctcagcctta tctggcaggg cagcgcccct gcgggcgtca aagagaccta taaacaagcc
     1681 gccactggcc gcgagtcgat tgcactcgaa agcctcaacc cggccctgag cctgcagccc
     1741 tttagcatcg actttagcgc cttagccagc atcaatgatc gcctgacagt ggaggaaatg
     1801 cgggtgcaaa ccgcctttga aagtgcgggc cgcaaccgat tgagcctgca cccaaacgcc
     1861 ctgcaactgg ccattgacag cccgagcggg gctgacccag acctcattgg agcgaatccc
     1921 aacaccagcg aagcgaaccc cgacagcgga gcaaaaatag gcacctggtt taacgtggag
     1981 aacaaaacca acctgatctt gagtgggcaa gcgggggatc aacttaacct gagcggtagc
     2041 agccaatggg cgggtaccct gagtctggag aaatttgcca tcactgccca gggcgagcag
     2101 agcctagcgg cggggcagat taacttcaac agcgtcgcga gcatcggcag cgcgaaaacc
     2161 gcagtgcaga tcgccactcc cctggtgcag ttgaataacc tgcgcggcga tgcctgggtg
     2221 gcgagcagcg gtgatattaa actggccagt ctggacgggc agggcagact taatctgcgc
     2281 gccgatggtg ttgtcggtca aacccaggcg ctcagctata cccgcgattt gacccttgcc
     2341 gccggtggtg ccatcaatct ggataatccc gacaatcgct taacgggcgg actgtccctc
     2401 tctgcggcca atacgattac cctggccaat ggcctcacca gcatcagcgc cctcaacgct
     2461 gagcacttta cctacaccgg tcaggatctc accgttgccg ggcctatggt ggtggcgggc
     2521 aatacggatg taaccagtac cggtagtatc gacgccctca accccgcaaa ccatctagcc
     2581 cgcctgcggg taaataccgc aggtaccgcc gcattcgccc tgggggcgga tagcaccctg
     2641 gtgggcgcca aagtgggagg agccttgcac gtccaggccg atggcttgac ggtagccggt
     2701 gatctcacgg cagcgtcgct ggatcttatc gccggacagc cgggcactgg cgcgggccaa
     2761 ttaaaagtca gcggccaatt aaatgcccca cagatcagcc tcagcgctgg cgatatcgtg
     2821 ttaaagaccc agttgcaagg cattgatctg agcctcaatg cgaagtcggt cagcttagaa
     2881 aaggcaatcg acctacaggg caacttcagc gcccaagtcg atagcctgca gcaggcagcg
     2941 gcagcggaca tctctgctaa aggcgcattt acactcaatg ccagtggtgc tctgaatttg
     3001 gatggcgcca tcaccagcgg tcgggatatc tctattgcgg ccggaggcac cattcaacag
     3061 agtggcgctt ggcgcaccga gggcaccggc gccagtggcc aaaccaaacc ctctacggcg
     3121 gatgtagtgg tgaaatccgg tggcaacatc acccaacacg ccaatgccaa cacccaaagt
     3181 gccggtggca ttacctatga agccgcaggt gccgtcagcc tggctgccct aggcgcccga
     3241 cgcgaggtgg tggtgaatgc caccggcggc atcattgacg ccaacggcac ctccactaac
     3301 ataaccggcc ccagcgcgcg ccttatcagc ggcggcgata tcagcgggct ggatctctcc
     3361 gtggacagct tggtgggcca gttcgcaggc ggcgccaaaa ccatcaacct gcgcaatgat
     3421 aaaaccctgc gcattgaccg attgattgcc ggtagtgccg ccgaagctac cgtgacgact
     3481 atttccacca cccgtgggga tgtcattttt aataacgatg ccagtaaaaa ccccgaagtc
     3541 gcgaaccttt attcgaaacc cgaccttgcg cgggaaaaga ccggtgtggc gaatagtaat
     3601 ttcagcacag gcacgctgaa catcctgtcg gaaaaaggcc gggtaaaagc cacgggctca
     3661 gtcaatatca accagccgga tttggtggcc aataacgtga atattttctc cgcgttgggt
     3721 gtgagcactc cggggcgtcc cttggtgatc tacagcgcag gtaaggtgtt gtacaccggt
     3781 acaggcttaa actggaagcc ttttacggcc ttcggccagc gctttgaatt taccagcgac
     3841 aatgcattgg atttatcgca gttattgttg gccgctgggg atcaattaat cgaaattgaa
     3901 cccttagaag atgtcaatcc agcggtgttt acggatatcc gtgtgtacac gtctggaacg
     3961 aacgcgatat tgcttcctgg agatcagcgc tacgaggaat aattccgtgt ggtaataatc
     4021 cagtaaattt aggcgattta tagcgtgacg ttttatacag cagacttttg agtgaagtga
     4081 tttcattact aataaatgaa tcgggttacc tataatttgg gtggtctgat tgaaattaat
     4141 atttataaat acagtaaaaa tggagtgaat atgattaagg tagccattca tgcgtcggtc
     4201 ggcagcaaag ggaaaaatat ggaaaaggac atcaaaacgg taaccgcgct gattaatgtg
     4261 tatcgacgta gcctaaatca gacgtcaatt gttatgagtg ccaagacgtc gacggaattg
     4321 gaaggtgcta ttggcgaatt tcagtctaac caccttaagt caaagtcccc tgatagcaaa
     4381 gtagacgcag gcggtaaaac attcgcggcg ctcaagcttt tctaccagac gtttttcaaa
     4441 gccattgcac ttacgccgcc cacctatggt gaggtcacct ggaattctga aggggcagaa
     4501 ggaggcaaat accatagccg atgttttacg gtgccctcgt cttcgtcggg attaaccata
     4561 gggcggggct acgatatgaa ggagaagtcg caggctaaaa tagccagtga tttgacgtca
     4621 attggacttg ctgcatctca atccgatacg attaaaaagg cggctgggtt aaatggtgcc
     4681 cctgcggaat actttgtcgt ggagaatgat ttgctcgatt ttcagttgac gccagcacaa
     4741 cagcttgcgc tgtttaagat tagctacgat gagcaagcag ctgaggtgaa acgtatttgt
     4801 gggttagata aaaatgtaaa ggactatggt caggttgatt ggattaagct tgacagcagc
     4861 atcaaagata ttgccataga ccttaaattt aggggggatt acacaccggc tagtcgtaaa
     4921 ttgatccaaa aagcgattgc ggataatgat cttgccgaat ttaaaaaaca gcttaaaaat
     4981 gccgtgaact ggccaaatgt acctaaagat cgctttgatc gaagggtgaa atttttagat
     5041 gagcactaat tgccttatgt caaaaattaa attgagtact ctgtccattt taacgtactt
     5101 atgtggctgt actaacgttt acgctggtgt ttcggtccct ttatctgttg attctgtgca
     5161 gactaaacgt gcgaaaagtg tcttaattcg tgtcattcaa tacaatacgg ataccgctgc
     5221 acttcgattg gaaagtatta aaccgataga acatgtgctg ctggattctg ttgacgtgag
     5281 tgcttttacg gttgatggtc gcaaatacga gttttctaaa tgcgatgaac tttcggttaa
     5341 ttctgtggcg gttgaagcgg agcaagtgtc tgctgaattc gagtgctttg tgccaaaggc
     5401 aacggctatc gtggcgaaat gcagtattcc cattcgggcg gataaattcg atgcgatgag
     5461 ctgtgttaaa gcggctcgcg aatgatttag gcggtgtgtt tagtcgtagt cactatttgt
     5521 gaggatgatc aaccagcacc ttagttcagt gactctgcac ccagtagatg gcgaacctct
     5581 aggtctcggc gcaacacaag ccccggcagc atctttccac ccccgtgaac ccaacgcttg
     5641 agttcacgtg cggcctccac ccagttcttt tgattaatgc gcagacgcaa tgtggacgtt
     5701 tgcaggcggc ctgcgccgag gttaaaggta aaatcgacga tggccgcgag ccggtgttcc
     5761 ggctcttgga tcaggacggg acaaagacgc agcgtggcat tgagggcggt gcttaagtcc
     5821 tgcgccaggt actgctccgc ctccagttca gtgatggcag gatggttggg tgtgcacaga
     5881 tggccatagc caatcgtcca aaaacccgcc ggac
//
LOCUS       DAOQGS010000234         5888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6802_length_5888_cov_5.307732, whole genome shotgun sequence.
ACCESSION   DAOQGS010000234 DAOQGS010000000
VERSION     DAOQGS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6802_length_5888_cov_5.307732"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKZ52_09850"
     CDS             complement(<1..308)
                     /locus_tag="PKZ52_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60120.1"
                     /translation="MSTTIAALDALKRQINRAERDLIEQRDGDGVEGDEIALLFTGNW
                     HDSIPRRIILSNADDGSQTLSPVERLTWMVFRVTINDHTKPGSMPRRADLASMIGCST
                     T"
     gene            complement(318..875)
                     /locus_tag="PKZ52_09855"
     CDS             complement(318..875)
                     /locus_tag="PKZ52_09855"
                     /inference="COORDINATES: protein motif:HMM:NF022645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4526/YPO1902 family pathogenicity island
                     replication protein"
                     /protein_id="HNG60121.1"
                     /translation="MRTDFYNLFNQQVLYLAVSALKADNEKLIAHLGLSRMAEDTRQQ
                     ISNLSLSQLVHVADFRGLLAEVRFNESQLINYLKMAASKTVEDQLIDEAIVGGMRQPM
                     LQTLKGISRREFNSRREKLGVSETGRGRIGNLDLDEDEELKVIKVWQELIHKTDDMLE
                     RYVLLHRETGLPLDKAQNTIQQLFA"
     gene            complement(919..2625)
                     /locus_tag="PKZ52_09860"
     CDS             complement(919..2625)
                     /locus_tag="PKZ52_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60122.1"
                     /translation="MKDVSSLLNRPHFQAGGGDGGGELPPDPAVKTRIKVNLDQVIAF
                     QGNPRKSQNPAYAEIKESIRKIGLQNPPNVTRADPSHPYMIRDGGNTRLQILKELWDE
                     THDRKFYEFECDFHPYSNHLDLLIKHVIENEMRGGMILIERGLAALEAKKEFEIRDKK
                     SISNSGLSRELRDLGWAINDRSLSYAIFAAETILPVLPKALWGGMGRPSIESIRKLLD
                     DCSTFWMNLPEHKDDPETASEEFAMLWQNVFTEEDDEHISISELQDALEGAIADYIGC
                     PLMSVTGEIQAISKGLSKGGIRPESVLLEESLKPQASASSASSAVAPSNEAPTRSSTP
                     KPYVKSQQNAGESEFHTNPASNYTYTPTMVDKESGLIPEPEAAKSFHEAADIHRPEAV
                     SIPDLLPNLPPSAEYGTFTLEELHNRAYGYAYQISDYLGLSDLIVPGDTLAEGKPHVG
                     FFMTKPEQGDYFDAYEESPELRQVGNYYRYLFFLSCSPLTHVPPREDTAPIHAMDLGW
                     LTNWPTQVMGVRQLIEFISLRCQYQARPDPDSLEAILIKLTCLLEKVVGEILLRLRDS
                     QA"
     gene            complement(2622..3554)
                     /locus_tag="PKZ52_09865"
     CDS             complement(2622..3554)
                     /locus_tag="PKZ52_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNG60123.1"
                     /translation="MKIFGIISTKGGVGKTTLTANLGAILADMGQSVLLIDADPQQTL
                     SRYFDITEAAPFGLTQLFKASNPARTISKTSIPGLDIVICDDKKADGAIPGLLRESIT
                     NYQNLSVALRQVEGQYDYILIDTQGTTHTIQDAVIYAADVLLSPTPPKVLDTREFIFG
                     TVELVKKYQPRPGFISILGRPTPPVRAIINLWDRTNSAQQIIKYLRAEFDREADSAIT
                     VLQTIIPAKNVYSVSGGEGVPAHRRDFIRRGNSPSALEVLLSLVHELEPKLSHIVPNW
                     EGIPKAALQQLQEHATDTGHSDVSAEEIAVGGDQ"
     gene            complement(3650..3726)
                     /locus_tag="PKZ52_09870"
     tRNA            complement(3650..3726)
                     /locus_tag="PKZ52_09870"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3690..3692),aa:Met,seq:cat)
     gene            complement(4228..5838)
                     /locus_tag="PKZ52_09875"
     CDS             complement(4228..5838)
                     /locus_tag="PKZ52_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNG60124.1"
                     /translation="MLRLSEIALPLNHAESALPAAIVAKLGIAASELIRYNVAKRAVD
                     ARRKSNIQLIYNLDITLDPAAEDRLLAERNPHVRPAPDTRYKFVAQAPEHLAERPVVV
                     GLGPCGIFIALVLAQMGFKPIVLERGKNVRERTKDTWGLWRKKVLNPESNVQFGEGGA
                     GTFSDGKLYSQVKDPKHYGRKVLNEFVAAGAPEEILYISKPHIGTFKLVTMVEKIRAE
                     IIALGGEIRFSTRVDDIARQDGQITGLHLSSGEFLPCRHVAFAVGHSARDTFAMLYER
                     GVYIEAKPFSIGFRIEHEQSKIDKCRYGDNAGNPILGAADYKLVHHCANGRSVYSFCM
                     CPGGTVVAATSEEGRVVTNGMSQYSRNERNANSAIVVGISPEEDYPGHPLAGIDLQRR
                     LESGAFELGGGTYDAPAQRVGDFLAARPSTQLGSVKPSYTPGITLTDLSTSLPTYAIE
                     ALREAIPAFDRQIRGFASPDALLTGVETRTSSPICIKRGGDFQSINTQGLYPAGEGAG
                     YAGGILSAGIDGIKVAEAMALAMLESFG"
BASE COUNT         1413 a         1518 c         1505 g         1452 t
ORIGIN      
        1 gtggtagagc atccaatcat cgaggccaaa tcggccctcc gtggcatcga gccgggtttg
       61 gtatggtcat tgatggtgac cctaaacacc atccatgtca ggcgctcaac aggtgaaagg
      121 gtttgactgc catcatcggc attgctcaag atgatccggc gagggattga gtcgtgccag
      181 ttaccggtaa ataacaaagc aatctcgtca ccttcaacgc catcaccgtc acgctgctcg
      241 atcaaatccc gctcagcccg gtttatctga cgtttgagcg catccaacgc cgctattgtt
      301 gtgctcatgt aattactcta ggcaaatagt tgctggatag tattttgggc tttgtccaat
      361 ggcaggccgg tctcacgatg caacaacacg tagcgttcca gcatgtcgtc cgttttgtga
      421 atcaactctt gccagacctt gatcaccttc aactcttcgt cctcatcgag atccaaattc
      481 ccgatgcggc cgcgaccggt ttcactcacg ccgagttttt cacggcgcga attaaactca
      541 cgacgcgaaa taccttttaa ggtctgcagc atgggctgac gcattccacc aacgatggct
      601 tcgtcgatga gttgatcctc tacggttttt gatgcagcca tttttaaata attaattaac
      661 tgcgattcgt taaatcgcac ttccgcgagt aggccgcgaa aatcagcgac atgaaccaat
      721 tggctcaaac ttaaattgct gatttgctgg cgggtatctt cagccatgcg cgataaaccc
      781 agatgggcaa taagtttctc gttgtcggcc ttcaatgcag aaactgccag gtataagact
      841 tgttggttaa acaagttgta aaaatcggta cgcatacatc ctctccatcg caaataaatg
      901 aaagtgtcgt taaatggctc atgcctgtga atcgcgaagg cgcaggagaa tttctccaac
      961 gaccttctcc aacaggcacg tgagctttat caggatggct tcgagactgt cgggatcggg
     1021 ccttgcctgg tactggcatc taagcgatat aaattcaatt aactgacgca cacccatgac
     1081 ctgggtcggc cagttggtca accaaccgag atccatggcg tgaatgggtg cggtatcttc
     1141 ccgaggaggg acgtgagtga gaggtgagca acttaaaaag aacaggtatc tgtaataatt
     1201 accaacctgt cttaattctg gcgactcttc ataagcatca aagtagtcac cctgttctgg
     1261 tttcgtcatg aaaaatccta cgtggggttt tccttctgcc aaggtatccc cgggcacaat
     1321 taaatccgac aaccccaaat agtccgatat ttgataagca taaccgtaag cccggttatg
     1381 taattcttcg agcgtaaacg tgccatattc agctgacggt ggtaggttcg gtaataagtc
     1441 aggaatagat acagcttctg gacggtggat atcagcagct tcatgaaacg atttagccgc
     1501 ttcaggttca ggaattagac cgctctcctt atcaaccatc gtcggggtgt aggtgtaatt
     1561 ggatgcagga ttagtatgga attcagattc gcccgcattt tgctgtgact ttacataggg
     1621 tttgggcgtg gatgaccgag taggtgcctc gtttgaaggc gcaaccgctg atgacgctga
     1681 tgaagcagaa gcctggggtt taaggctttc ctccagcaat acagattccg gccgaatgcc
     1741 acccttactc aaccccttgc taatggcctg aatctctccg gtaaccgaca taagcgggca
     1801 gccgatatag tcagcaatgg cgccttctag cgcgtcctgc agctcactaa ttgaaatgtg
     1861 ttcgtcatcc tcctccgtaa agacgttttg ccagagcatt gcgaattcct cactggcagt
     1921 ttcaggatcg tctttatgct cgggcaggtt catccaaaaa gtactgcagt catcaagcaa
     1981 ttttcttata ctttcaatcg aggggcgccc catgccaccc cataaagctt taggtaaaac
     2041 gggtaaaata gtctctgctg caaatatggc gtaacttaaa cttctatcat ttatcgccca
     2101 tcctaaatct cgtaattccc gagatagtcc tgagtttgaa attgattttt tatctctaat
     2161 ttcaaattct ttttttgctt ctaaagccgc caggccacgc tcaataagga tcattccgcc
     2221 acgcatctcg ttttcgataa cgtgtttaat cagcaaatca aggtgatttg agtaaggatg
     2281 aaagtcgcac tcaaactcat agaatttgcg gtcatgggtt tcgtcccaca actccttcaa
     2341 tatttgaagt cgagtattac caccatcccg aatcatgtac gggtgactgg gatcagccct
     2401 cgtaacatta ggtggatttt gcaaaccaat tttacgaatt gattctttaa tctcggcata
     2461 ggccggattc tggctcttgc gggggtttcc ctgaaaagca atcacctgat ctaaattcac
     2521 cttaattctg gttttaacgg caggatcggg tggcaattca ccaccgccgt cgccgccacc
     2581 agcctgaaag tgtggccgat tcagcagaga ggagacatct ttcattggtc acctccaacg
     2641 gcaatctctt ccgccgaaac atcggagtgt cctgtgtccg tagcatgttc ttggagctgc
     2701 tgtaaggccg ctttgggaat gccttcccag ttgggcacta tatggctgag cttgggttcc
     2761 aattcatgta ccaagctaag caagacttct agggccgatg gtgagttacc tcggcgaatg
     2821 aagtcgcggc ggtgtgcagg tacgccttcg ccaccactga ccgaatatac atttttagcc
     2881 ggaatgatcg tttgcaatac ggtgattgcg gagtcagcct cgcgatcaaa ttcagcacgt
     2941 aggtatttaa ttatttgttg cgcgctgttc gtccgatccc ataaattaat gatggctcgg
     3001 acaggcggcg tggggcggcc tagaatggaa ataaaacccg ggcgtggttg gtattttttc
     3061 accagttcaa cggtgccaaa aataaactcg cgggtgtcca acaccttggg gggagtgggg
     3121 ctgagcaaca catctgcagc gtaaatcact gcatcctgga tagtatgtgt agtgccttgt
     3181 gtatcgatca gaatgtaatc gtactgacct tcgacctggc gaagtgcaac actgagattt
     3241 tggtagttgg taatggactc gcgcagtagc cctggaatag cgccgtcagc cttcttatcg
     3301 tcacaaataa cgatgtcgag acctggaatg gacgtttttg aaatggttct ggcgggattg
     3361 ctagccttga agagctgagt gagcccaaag ggagccgctt cagttatatc aaaatagcgt
     3421 gagagtgttt gttgtgggtc tgcgtctatg agcaaaaccg actgacccat gtcagcgagt
     3481 atcgcaccga gattagcagt cagcgtggtt ttacccacac cacccttggt agaaatgatt
     3541 ccgaaaattt tcatgttagc ctctgcaacc gtttggatga gttgaagaaa caacagaaaa
     3601 acgcaatcga ctttctttac ttacctacac atcgtgacca ccgattaaat ggtggctaga
     3661 gcgagactcg aactcgcgac cccagcatta tgagtgctgt gctctaaccg gctgagctac
     3721 ctagccgatg tgttgccccc ttaaggaggg gcgccattct cgcgatcagg cctaggcttg
     3781 tcaactgctg agggcaagtt tttcaatgca aggtcgaggt aggtttgcgc tcttttactc
     3841 gtgtatttgg cctggctcga ttgcgcccaa ccgaaaaaat tcacccgcaa catcccgccc
     3901 ctctgttcag gataaagctg tgggtgtcat ccttaaaaac cccaaaaacc ccaaaaaccc
     3961 ggtcagtatc ggcaggctat caattcttat acagacaaaa ccctggctct gacagaagga
     4021 ttggattgca aaaagccaac atcaggatta gctatggaag gtgaacgtgc acagcaaacc
     4081 ctaggcgcca ctgacagcac ttaggcttgc gaccccagca ttgtaagtgc tgtgtcccga
     4141 cgaccccaat cgagaaaacc ccgcgataac gatatgacag ggtgaaaggc taatcagcaa
     4201 ggtttgctat gactgcgccg agcacgccta gccgaatgat tcaagcatcg ctagagccat
     4261 ggcctctgct actttaatac catcaatccc cgccgagaga ataccccccg cgtaacctgc
     4321 accctcaccg gctggataca agccctgtgt atttatgctt tgaaaatcac ccccacgctt
     4381 gatgcaaatg ggcgaggagg tgcgggtttc tacgccggta agcagcgcat ctgggctggc
     4441 aaaacctctg atctggcggt caaacgccgg gatcgcctcc cgcagggctt cgatggcata
     4501 agttggtagc gacgttgata ggtcggttag ggtgatgcct ggggtatagg agggtttaac
     4561 tgagcccaat tgggtcgagg gccgcgccgc cagaaaatcc cctacccgct gggcaggtgc
     4621 gtcgtaggtg ccgccgccca gctcaaacgc gccggactcg agcctgcgct gcaaatcaat
     4681 acccgccagc ggatggcctg gataatcctc ctccggtgag atgcccacca ctatggcact
     4741 gttggcgttg cgctcgttgc gggagtactg gctcatacca ttggtaacca cccgcccctc
     4801 ttctgaggtg gctgccacca ctgttccgcc ggggcacatg cagaagctat agaccgagcg
     4861 gccattggcg cagtggtgga ccaatttgta gtcggcagcg cccaaaatag ggttgcccgc
     4921 gttgtcgccg tagcggcatt tatcaatttt ggactgctca tgttcgatgc gaaagccgat
     4981 ggaaaacggc ttggcttcta tgtagacgcc gcgctcatag agcatggcga aggtgtcgcg
     5041 ggcgctatgg cccaccgcaa aagccacgtg gcggcagggt aaaaactcgc cgctggataa
     5101 atgcaggccg gtgatctggc cgtcttgccg cgcaatatca tccacccggg tgctaaagcg
     5161 aatctcaccg ccaagggcaa taatttcagc gcggattttc tccaccatag tcaccagttt
     5221 aaaggtgcca atgtggggtt tgctgatgta gagaatttcc tccggcgcgc cagcggcaac
     5281 aaattcattg agcactttgc ggccgtaatg tttggggtct ttgacctggc tgtagagttt
     5341 gccatcggaa aaggtgcccg ctccgccctc accgaactgc acattggatt ctgggttgag
     5401 cactttcttg cgccacaggc cccaggtgtc cttcgtgcgt tcgcggacat ttttgccccg
     5461 ctccagcact atgggtttga agcccatctg agccaacacc agggcgataa aaattccaca
     5521 ggggccaagg cccaccacca ccggccgctc ggcgaggtgc tctggcgcct gggcgacaaa
     5581 cttgtagcgg gtgtccggtg ctggccgcac atgggggttg cgctcggcga gcaacctgtc
     5641 ctcagcggct gggtcaagtg taatgtccag gttgtagatg agctggatat tgctcttgcg
     5701 gcgggcatca acggcgcgtt tagccacgtt ataacgaatt aactcgctgg cggctatgcc
     5761 cagtttggcg acaatcgccg caggcagcgc gctttcggca tggttaaggg gcaaagcaat
     5821 ttcactaagg cgcagcataa gcagtcacag ctaaaaattc gggcggcatt ttacgccagg
     5881 ccattgca
//
LOCUS       DAOQGS010000235         5863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6844_length_5863_cov_6.916494, whole genome shotgun sequence.
ACCESSION   DAOQGS010000235 DAOQGS010000000
VERSION     DAOQGS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6844_length_5863_cov_6.916494"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKZ52_09880"
     CDS             <1..1049
                     /locus_tag="PKZ52_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60125.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGSGGPVLT
                     INQRDITAYFSRFACGVDFSAWGSGYSACFRTPTGGAQCIENSEGTPRTIVWQDSRQP
                     VGNVLRYYGGGIKFGGALLITDSGQAFAGFSGNVYPNPIINAGAISGAVAYRQHCVIV
                     KNQQRGEVSCYTNTDGSNLKSVTGLPANFDAIQLAADDHTDNKMCALGTNGRAWCWQW
                     ESGDNFFPAAEVNFSAPVKSISVGYRGVCGVKFSGGIECTNGWSAAPETLPNAQLTYQ
                     SNNQLITLNENAQIFLNTTEGVTKLSDQGAGVGVNFFYGAFTQTSDGSVFALDNNQLK
                     QLPSLKAQAAQCPF"
     gene            complement(1678..2355)
                     /locus_tag="PKZ52_09885"
     CDS             complement(1678..2355)
                     /locus_tag="PKZ52_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60126.1"
                     /translation="MKKLIKSSPAYILGILLVSGLCKFAYSEELKNSEDDIFARSVQS
                     IIDSSRKCNHGEFLSLVAKQGVVASRYYSAGIQNVSFVKAFSVNIIPKTIDFSIGTKR
                     AGKLTVHGMLDTLKDTGVILDPSKCKLHSVNLQAINLDAVLNNKSANSKTAEIAKLYY
                     QAFSLDNNAKRDMAQVCVTNKEHSQAYFGLVSTPEDGTNELIFGAALLFEKENNQWRL
                     RGVAEFL"
     gene            complement(2760..3473)
                     /locus_tag="PKZ52_09890"
     CDS             complement(2760..3473)
                     /locus_tag="PKZ52_09890"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNG60127.1"
                     /translation="MPHSHCGHYNAATTTPRPALRVYLLNSYLSGIFGLVYVPSTLID
                     WTNPAVTVANIKGAERLFRLGILSEIFCFLAFIALPLLLYELLKCAGRVAAALMAIFS
                     LICVPMAFINIVKKVDVLLLLGGEPYLSAIPPDQLQAQVMYLLASFNNGTLVANIFWG
                     AWLFPFGYLVYKSDLLPKILGIFLILGGIGYITEFLVRFLFSGTQIPWYISLPGSIGE
                     FGICLWLLIMGVKERPLGA"
     gene            3718..4227
                     /locus_tag="PKZ52_09895"
     CDS             3718..4227
                     /locus_tag="PKZ52_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60128.1"
                     /translation="MSQKLFAIMALMALSACSQQPSQPQQIQTSAVNQKGLEFLVEIQ
                     TRNFAKSADYCAQQSASFKPAFANYINNYRQGTAIALQKIGPQEGLNLAPDEKEMPTF
                     MALQDLQGEDMLKNVKANPSAGCAKIMGIFTAMTVQAAERQMHTHFAEYTARRREFCS
                     KQPKPANCP"
     gene            4723..5706
                     /locus_tag="PKZ52_09900"
     CDS             4723..5706
                     /locus_tag="PKZ52_09900"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNG60129.1"
                     /translation="MNCIIGIDVSKDKIDVAWLRDIHTLKVKTKVFKNTPSGFAQLSS
                     WLSENTKQPLNNCHCSLEATGIYHEPLSHWLFEQGISVSVCNPAQVKNFAKSLGAQHK
                     TDKIDSIILARYGALMKPALWQPEPKAVRELKALIARLEAIESDLQREQNRMEKAEFS
                     AASELVIESLNTMIQHLESERKKLDQEIDDHIDRHPDLKRDQELLQSIPSVGRVVSRL
                     MLFVIRGKDFKTAGDCAAYVGLIPKIQESGVFKGRAALSKKGNSRIRAKLYLPAVTAM
                     RHNPSIEQHTKRLLEKGKNKMQAIGAAMRKLVHICFGVLKNQKPYEFRAAV"
BASE COUNT         1612 a         1350 c         1390 g         1511 t
ORIGIN      
        1 catcgtcttc atcgtcttca tcgtcttcat cgtcttcatc gtcttcatcg tcttcatcgt
       61 cttcatcgtc ttcatcgtct tcatcgtctt catcgtcttc atcgtcttcc ggatcgggcg
      121 ggccagtatt aacaattaat cagcgcgaca taacggccta tttttctcgc tttgcctgcg
      181 gcgtagattt tagcgcttgg ggttctggtt actcagcctg ctttagaaca cctactggcg
      241 gggcacaatg tattgaaaat agtgaaggta cacccagaac aattgtgtgg caggactcgc
      301 gccaacccgt tggcaatgta ttgcgctact acggcggcgg aattaaattt ggcggtgcgt
      361 tgttaataac agacagcggg caagcatttg cggggttttc gggcaatgtc taccccaacc
      421 cgattattaa cgctggcgcc attagtggtg cggtagctta taggcaacac tgcgtgatag
      481 taaaaaacca gcagcgcggt gaagtctcct gctacaccaa cacggatggc tctaatctta
      541 aatcggttac aggtctgcca gccaacttcg acgctattca attggcagcc gacgaccaca
      601 ccgataataa gatgtgtgca ctcggcacga atgggcgggc ctggtgttgg cagtgggaat
      661 ccggcgataa ctttttcccc gccgccgaag ttaattttag tgcgccggtt aaatctattt
      721 cggtaggcta tagaggcgtt tgcggggtta aatttagcgg tggtatagaa tgtaccaacg
      781 gttggtcggc ggcacctgaa acgctaccta atgcccagct gacctatcag agcaataacc
      841 agttgattac attaaatgaa aatgcacaaa tcttcctgaa tacaaccgaa ggcgtaacca
      901 aactttcgga ccagggggca ggagtcggcg tgaacttttt ttatggcgca tttacacaaa
      961 cttcggatgg tagtgttttt gcgctggata ataaccagct aaaacagctc cctagtctta
     1021 aagctcaagc ggcgcaatgc cctttttaaa cgcggcttac ctgtacaact caatttaagt
     1081 attatcgctg caagggcgat cattaatatt ctcgttccca tgctctgcgt gggaacgcag
     1141 gttctcttac aaatcaaggg gtcagagaca aatggcgcta agttaagcat agtttgggag
     1201 ttgtaaacgc gatgagaagg gcaaaaatac tgactcggag cagctgaact tagtgccatt
     1261 tgtctctgac cccttgatta ccaagcaccg gggctaccgc ctcgtctttg aactctttgg
     1321 agtagtgttt gtagatgcgt ttctggctca tgtgacacct caatggttga cggtttattg
     1381 tctttcatta gagtgtctgg gggtattaga gcactacagg cgtattcaat ataccggtaa
     1441 gctcccgggt cggaaggcac catcggcttt ttcgacctag gtaaatccaa aaacaatctg
     1501 ggttgcgcgt ttttttcaaa gtaactcgga tatttgtatt tgcgtagggc ggattcggag
     1561 cgaagcgaca atccaccaat tcgtggctat ctcgcgattc aaaataaaaa ttctcggttg
     1621 aaagaccgat ggtgggttac ttcgcaaacc caccttacag ttgaccaacc ctacgcgtta
     1681 taaaaattca gctacccccc gaaggcgcca ttgattattc tccttttcaa agagaagcgc
     1741 ggctccaaag atgagttcgt tggtcccatc ttcaggtgtg gatactaagc cgaaatacgc
     1801 ttgcgagtgc tccttgttgg tgacgcaaac ttgagccata tcgcgcttag cattgttgtc
     1861 aagcgaaaac gcctgatagt acagcttcgc tatttctgcc gttttactat tggcggattt
     1921 attgtttagt actgcgtcga ggttaattgc ttgcaagtta acagagtgca gtttgcattt
     1981 agacgggtct aaaatcacgc ctgtatcttt tagcgtgtct aacatgccat gaacggtgag
     2041 tttccctgct cttttggttc cgatagaaaa atcaatcgtt tttggtataa tgtttactga
     2101 aaacgccttt acgaagctga cgttttgaat tcctgcacta taataacggc tcgcgaccac
     2161 gccttgtttt gctacaaggg acaaaaattc gccatgatta cactttcttg agctatctat
     2221 tatcgattgc acgctgcgag caaatatgtc gtcttcagag tttttaagct cttctgaata
     2281 tgcaaattta cacaatccgg agacaagtaa aatcccaagg atgtaagctg gtgatgattt
     2341 aataagtttt ttcatatctc aattcctaat ttaaattcca cattttgatg acccctagca
     2401 acggtggtta aagtggaata acaatagcgt attccgtcaa tggtaatacc tccatcgcga
     2461 tctgagcctt tgctattgta ccatgcctag ctaaatcatt cttgtacgcg aatgccaagc
     2521 cttcggagag ttacttttct ttgggcgcgc aaagaaaagc gactcgccga aggagcggtg
     2581 cttgggtaaa agaattattt cggtattaga gagggtatgc attctcacgc ggagagtggt
     2641 aaagagaaat ggtgggttgc ttcgcgaacc caccttgcga gttgcttcgc gaacccacct
     2701 tgcgagttgc ttcgcgaacc caccttgcga gttgctgctc aacctgcatt ttgctcaatc
     2761 tacgcaccca acggccgctc tttcacgccc attattaaca gccaaaggca aatcccaaac
     2821 tctcctatgc tgcctgggag gctgatatac cagggtatct gtgtgcctga gaataagaac
     2881 cgcaccagga actcagttat gtagccaata ccgccaagaa tcaaaaaaat gcccagtatt
     2941 tttggcaaca ggtctgactt gtacaccaga taaccaaacg gaaacaacca agcaccccaa
     3001 aagatgttcg cgaccagggt gccattatta aatgaggcca ataaatacat tacctgcgct
     3061 tgtaattgat ctggaggaat tgcgctcagg tagggctcgc cgccgagtaa taacagcaca
     3121 tcaacttttt taacaatatt gataaatgcc atgggaacgc agatgagcga gaatattgcc
     3181 ataagtgcgg cggcaactcg ccctgcgcac ttgagcagct cgtacagtaa aagtggcagg
     3241 gcaataaagg ctaaaaagca gaatatttca cttagtatgc caagcctaaa taggcgctca
     3301 gcgcctttaa tatttgccac agtaacagcg ggattagtcc agtctattag ggtggaaggc
     3361 acgtatacca ggccgaatat tccgctcaga taagaattaa gtaggtaaac ccgcagagcc
     3421 gggcgagggg ttgttgtggc agcattgtag tgtccgcaat gtgagtgggg catcccgtat
     3481 aagacgctgc aaaccttagc gtggattcag ccttgggggc aactttctct atatttacaa
     3541 tttaatcttc gagcaggcgc aaagtggata tgggatattt aaacgcgcag cttggatttt
     3601 tgggatatac gtagggtgga ttcggagcga agggacaatc cacgattttg gattgaaggc
     3661 aacatatcgc acgctagcct ataatatgta atcacgcccc ttacccatgg acccattatg
     3721 tcccaaaaac tgtttgccat catggcgctc atggctctat ctgcctgcag ccaacaacca
     3781 agccaaccgc aacaaattca aacttccgct gtaaaccaaa aagggttgga atttttggtg
     3841 gaaatccaaa cacgtaattt cgccaagtcc gccgactatt gcgcgcaaca atcggcatcg
     3901 tttaagccag catttgccaa ctacattaat aattaccgcc agggaacggc cattgcgctg
     3961 caaaaaattg gcccccaaga aggcctgaac ctggcgccag atgagaaaga aatgcccaca
     4021 tttatggctc tgcaggattt acaaggtgag gatatgctca aaaacgttaa agccaaccct
     4081 tcagcaggtt gcgcaaaaat tatgggcata tttacggcca tgacggtgca ggcagccgaa
     4141 aggcagatgc atacacattt tgcagaatat accgccaggc gtagagaatt ttgcagcaaa
     4201 caacccaagc ctgctaattg cccctaaaaa tcgcctgccg gaatcttttt ggtgttagtg
     4261 tacgtgtgcg tttcagcgcg gtgcatggtg cgagaataaa ggcaaaacgc aagacctgac
     4321 cccaattttc aatcttgagt tctgttaata acggcttcag gtgattaaat gcgaattatg
     4381 tagaggaatt taacaagcca tccatcaggc caggccgtag gttgggcagt aatgcccaac
     4441 acaccccgct cttcgaataa aataatttaa actcgggttg cacaaaataa atctgtgcag
     4501 gctttggttg cgaaatgttg ggcattgctg cccaaccttg tatctctcca gtaagaaggg
     4561 gtagcctccc ataaggccaa agctgggttg agcgtgggcc caccctcaag cttaggagct
     4621 tgttttgtag attacgcccc cggcttttct ttcaaaataa attggatggt atcttagcgc
     4681 cgggtttgcc cgtgatgctg catagacaag ggtaattatt gaatgaactg tatcattggt
     4741 attgatgtaa gcaaagataa aattgatgtc gcatggctac gcgatatcca tacattaaaa
     4801 gtaaaaacca aggtattcaa aaatacccca agtggatttg ctcaactgag ttcctggttg
     4861 agtgagaata ccaaacagcc tttaaacaac tgccattgca gcctagaagc cacagggatt
     4921 tatcatgagc ccttatcgca ttggctcttc gaacaaggca tatccgtctc ggtgtgtaat
     4981 cccgcgcaag taaaaaactt cgccaaaagc ctcggcgctc aacataaaac cgataaaatc
     5041 gacagcataa ttctcgcccg ttacggggca ctgatgaaac cagcactgtg gcagccagag
     5101 cctaaagctg tgcgcgaatt aaaggcatta attgcccgcc ttgaggccat agaatcggat
     5161 cttcagcgcg aacaaaaccg tatggaaaag gccgagttta gcgcggcctc ggaattggta
     5221 attgagtcac tcaacaccat gattcagcac ttagaatctg agcgtaaaaa gctagaccaa
     5281 gaaattgatg accatattga ccgtcaccca gatttaaaaa gggatcaaga attactacag
     5341 agcatcccct cagttggacg ggttgtatcg cgcctaatgt tgttcgttat tcgaggaaag
     5401 gactttaaaa cggcaggtga ttgcgccgca tacgtagggc tcattccaaa aatacaggaa
     5461 tcaggagtat ttaagggacg cgcagcgtta agtaagaagg gtaactctag aattagagca
     5521 aaattatatc tgcccgctgt aacggcgatg agacacaacc caagcattga gcagcacacc
     5581 aaacgactgc tcgaaaaagg caaaaataaa atgcaggcga ttggcgctgc tatgcgcaag
     5641 ttggtgcaca tttgttttgg cgtattgaaa aatcaaaagc cttatgagtt cagggctgct
     5701 gtatagaaag gtcaatttaa atttattctg tcacgggtgg cgcagccagc gcgtagcgcc
     5761 ttgcccttga tagaatgaat ttaaattgta cgctggtgcg ggggttgagc catcaacccc
     5821 cgagcccttc cggcggtgag gaatggggtt tggggcgaag ccc
//
LOCUS       DAOQGS010000236         5828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6901_length_5828_cov_9.812229, whole genome shotgun sequence.
ACCESSION   DAOQGS010000236 DAOQGS010000000
VERSION     DAOQGS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6901_length_5828_cov_9.812229"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1377
                     /locus_tag="PKZ52_09905"
     CDS             97..1377
                     /locus_tag="PKZ52_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60130.1"
                     /translation="MNDAEYLNTLLGYLPPPQFRAFVQAEFNYCLPLFDDQTPYKVQR
                     EAFSAALSQIDVGRRQQMNEQAESILLLSDEVGQQVLEGFARDLGDKARYWQLTALPD
                     QYQRALWLYTHERSVFNDALQTRQADIVRNNLTCYAGFTAPKHLPVLDNREARARLQE
                     ELAQHLDCPATAVAIHIFKRLRLDPAENAVVDIIHIRIYHNKLPILVNCVHNGRLVSR
                     RIMRAEMSHITYEPANGHLEVATQSSVNREYLAHLVLRVLLPTAEAASTHSTHSTHST
                     SEESEGACLALKHYDFQRLSTPTVFDVSEEPIAHMKVIELGYSDFANRTFLIKINYQD
                     SDDIFTASKALISPIFDFSQYPLNYAKLAVRLKRTERERGRVISIILRGDNECTIKSK
                     HDQDRVLCDRLLHKWRLIKDRKERVSSAHGYVAA"
     gene            1343..2341
                     /locus_tag="PKZ52_09910"
     CDS             1343..2341
                     /locus_tag="PKZ52_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60131.1"
                     /translation="MYPPLTVTSLLELTRLFEYSSVFLNEQGQRLLGVPRWALAHACL
                     TPQERAHWLVSIGYASVYPVNLNGERVLADVRFDADANHYVCRSETSFRKMPVCEDDL
                     AILAVPEDRLMHSLADVCEVPIALRSGIQSPAIADVLWRLGKIRIQAVWIDLWCARHL
                     SINTAQIFRYLKNKQLPDKGLVLTCGAGLPEVIPPPRQYHFLALGQVICPRGNGCGID
                     GDLLHRVVGGAALPATDQSGPVRFDSYSNTLVITTKSIAPWSIKGARQQAVVRYLVDQ
                     LHKDRAWVPSHEILAYVYGAQQQGHSRRISDIFRGNLLWKDYITTSAKGLYGFKLD"
     gene            2624..2833
                     /locus_tag="PKZ52_09915"
     CDS             2624..2833
                     /locus_tag="PKZ52_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG60132.1"
                     /translation="MSIKHLNQAQLAERWGVSESTLERWRTDGIGPVFLKIQGRVLYR
                     LEDIQAYEADCLRKSTSESATGGVR"
     gene            2830..3321
                     /locus_tag="PKZ52_09920"
     CDS             2830..3321
                     /locus_tag="PKZ52_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60133.1"
                     /translation="MSLPLDQALGLAAQDLAHLAPDRLAELKQAAAECLAFAEAITHH
                     LDTAVNLRYGEHAQQLRASQGKNTGVVHFDDGAVRVSADLPSQIVWDQSRLAEIARRI
                     RANGDDPAEYMELRYDIPEATFNAWPDALKSAFTLARTRLAGTPTYRLALMRDAASAS
                     REG"
     gene            3325..>3821
                     /locus_tag="PKZ52_09925"
     CDS             3325..>3821
                     /locus_tag="PKZ52_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60134.1"
                     /translation="MTFFDFNSAEEQSTFDLIPRGTLVPVRMTFKPGGFDEPSQGWTG
                     GYATLGSTGAVYLTCEFVVLDGEFARRKLWSRIGLYSPKGPEWSNKGRTFIKALLNSA
                     RGIWPSDTSPAAQEARRIASFAELDGIVFIGRVDWERNKNGEFDNVIKTAITPDHAEY
                     AARVDA"
     assembly_gap    3822..3921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3994..4410
                     /locus_tag="PKZ52_09930"
     CDS             3994..4410
                     /locus_tag="PKZ52_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60135.1"
                     /translation="MTLKPPSIPWCDVFGTQDVLMATGLHAWICVLMMRGRWHAVRGD
                     DRHPIQRLALGDRQNCLAVAELWFQQVAPTDLGIIEHWMARPASPKQLTRLSPLLQTD
                     TPMTRYQASARLQFQYNAATLVRLVNTVASVAGEVL"
     gene            4554..4820
                     /locus_tag="PKZ52_09935"
     CDS             4554..4820
                     /locus_tag="PKZ52_09935"
                     /inference="COORDINATES: protein motif:HMM:NF039802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6511 domain-containing protein"
                     /protein_id="HNG60136.1"
                     /translation="MLDPVVMNTTALKACLNPLGQYLDAAGLNRPLADYSQDEVLALV
                     DTLVVAYRDSMLSQYEAALVQERLLYEHPQKRLDSPPVSRGVRA"
     gene            4817..>5828
                     /locus_tag="PKZ52_09940"
     CDS             4817..>5828
                     /locus_tag="PKZ52_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60137.1"
                     /translation="MSEYTPSDEQPSWTDFNDAPSGDSTDRFTAEDTDALRGALVDRL
                     DDILGYLLPQGRARGEKYYVGDLDGNAGKSLVVELAGPKRGLWQDFATGEGGDLFDLW
                     AAVRGLSARAHFPELVAEIRQWLGLVAHTPVSDLPGRSALIPSTVSPATATSAALSSG
                     PLGLPLAHSTPRPKVRVPVLDELGPYTAKWDYQTAQGELIACVYRYDPPDGKEFRPWD
                     VRARLWRTPDPRPLYNQPALSSAKHVILVEGEKCAEALIRAGLVATTAMNGAHAPVDK
                     TDWRPLKGKHVLIWPDRDAPGWDYAESAARACVLVGCASVAILVPPTDKPEKWDAADA
                     LAE"
BASE COUNT         1171 a         1668 c         1609 g         1280 t
ORIGIN      
        1 atttcaatca gttagcacgc gaaggagtct ggcaggttgc tctatggagg tagaggaggt
       61 cgttctttcg cagggatagg ttttagtgag atcaccatga acgatgcaga gtatttaaat
      121 acacttttag gctatttacc gcctccccaa tttcgcgctt ttgtgcaggc agaatttaat
      181 tattgccttc cgcttttcga tgatcaaaca ccctataaag tacagcgcga agcattttcc
      241 gcagccttaa gccagattga tgttggccgc cgccaacaga tgaacgagca ggcggaatcg
      301 attctgttgt tatccgatga agtcggacaa caggtgcttg agggctttgc acgcgatctg
      361 ggtgataaag cccgctattg gcagttaacg gcgctaccgg atcaatatca gcgggcgctc
      421 tggttgtaca ctcatgagcg ctccgtgttt aacgatgccc tgcagacgcg gcaggccgat
      481 attgtgcgca acaatctcac ctgttatgca ggcttcacag cccccaaaca tttgcctgtg
      541 ctggataatc gtgaagcgcg agcccgactg caggaggagc tggcccagca tttagactgc
      601 ccggccaccg ccgtcgctat ccatattttt aagcgtctgc gcttggaccc agccgaaaac
      661 gcggtggtag atattattca catccgtatt taccacaata aactgccaat cttggtaaat
      721 tgtgtgcaca acggcaggct ggtttctcga cgcatcatgc gcgcggagat gtcgcacatc
      781 acctatgaac cggccaatgg ccacctggaa gtagccactc aatcgagtgt caatcgcgag
      841 tatttagcgc atttggtgct gcgcgtttta ttgccgacag ccgaagcagc atccacacac
      901 tccacacact ccacacactc cacatcagag gagtctgaag gggcatgttt agccttaaag
      961 cactatgatt ttcagcgctt atcaacgccc accgtgtttg acgtgtctga agaacccatc
     1021 gcacacatga aagtgatcga actcgggtat tcggattttg ctaatcgcac gtttttaatc
     1081 aaaataaatt atcaggattc cgacgatata tttaccgcat cgaaagcctt aatcagtccc
     1141 atcttcgact tcagtcaata cccgttaaat tacgccaaat tagccgtgcg gttaaagcgc
     1201 acggagcggg agcgcgggcg cgtgatttcc attattttgc gcggtgacaa tgagtgcacg
     1261 ataaaatcca aacacgatca ggatcgcgtc ctctgcgacc ggttgctgca taaatggcgc
     1321 ttaattaagg acagaaaaga acgtgtatcc tccgctcacg gttacgtcgc tgcttgaatt
     1381 aacccgttta tttgaatatt catcggtatt cttaaatgag cagggacagc gcttattagg
     1441 ggtgccccgc tgggcgctgg cgcacgcctg cctgacacca caggagcgcg cccattggct
     1501 cgtatctatc ggttatgcca gtgtttatcc ggtgaatcta aatggggagc gggtgcttgc
     1561 ggatgtccgc tttgatgccg atgccaacca ttatgtgtgt cgatctgaaa cgtcctttcg
     1621 caaaatgccg gtgtgtgagg atgacctggc tattctcgcc gtgcccgaag atcgattaat
     1681 gcactctctg gcggatgttt gtgaagtccc tatcgcctta cggagcggga ttcaatcacc
     1741 cgctattgca gatgtgcttt ggcgtttagg caaaatccgc attcaggcgg tgtggattga
     1801 tctctggtgc gcgcgacatt tatccataaa caccgcgcag atttttcgct acctaaaaaa
     1861 taaacagcta ccggataaag ggttagtgtt gacctgtggc gcaggcctgc cggaggtgat
     1921 tccacctccg cgccagtatc attttttagc gttagggcag gtgatatgtc cgcgtggaaa
     1981 tgggtgcggc atcgatggag atttactgca tcgcgtggtg ggcggcgctg cgctgcccgc
     2041 aaccgatcag tccggaccgg tacgctttga ttcctacagc aacaccctag tgatcacgac
     2101 caagtccatc gcgccctgga gcatcaaagg tgcccgccaa caagcggtgg tacggtacct
     2161 ggtcgaccaa ctgcacaagg accgcgcctg ggttcccagc catgagattt tggcctatgt
     2221 gtacggcgca cagcagcagg gccacagccg tcggatcagc gatatttttc ggggcaatct
     2281 actttggaag gactacatca ccacttctgc aaaaggcctg tacgggttca aattggactg
     2341 acgcaatagg ccgctctcac gtcctatcca ctttcttacc ctgcccctac ccctgcccct
     2401 gcccgctccc ttagtctcac tcgtcactac ccaaggccat gtgtgcctta ctggtaacaa
     2461 ctaacgccat gcgccttcca gcgtgcgttg gtgtggttgc tcatcccttc gtttcagccc
     2521 ttttcttccc tcccctcccc ccacccattc gcagggcaga cgccccgcca tcgctctttg
     2581 gacactgcgc tcacgcatcc tcaataaccc ataggagcac gacgtgagta ttaaacatct
     2641 gaatcaagcc caactggccg agcgctgggg cgtcagtgag tccaccctgg aacgctggcg
     2701 aaccgacggc atcggccctg tttttcttaa aatacagggg cgtgtgttgt accgcttgga
     2761 ggatattcaa gcctacgagg ccgattgtct gcgcaagagc acctcggaaa gtgcgaccgg
     2821 aggtgtgcga tgagcctccc cttggatcaa gctctaggcc ttgccgccca ggacctcgcc
     2881 cacctagccc ctgatcgctt ggcggaactc aaacaggccg ctgccgagtg cctggccttt
     2941 gccgaggcga tcacccacca cctggatacc gccgtcaacc tgcgctatgg cgagcatgcc
     3001 cagcagttgc gggccagtca gggcaaaaac acaggggtgg tgcattttga tgatggggct
     3061 gtgcgcgtca gcgccgatct gccgagtcag attgtgtggg accaatcccg tttggcggag
     3121 atcgcgcggc gcattcgagc caacggcgac gatcctgctg agtacatgga actgcgctat
     3181 gacatccccg aggcgacctt caacgcctgg cccgatgccc tcaaaagtgc ctttacgctc
     3241 gcccgcacgc gattagccgg gacgcccacc tatcggttgg ccctgatgcg tgacgccgcc
     3301 agcgcaagcc gagagggttg agtcatgacc tttttcgatt ttaattccgc tgaagagcaa
     3361 agtacctttg atttgatccc ccgaggcaca ctggtaccgg tgcgcatgac ctttaagccc
     3421 ggtggatttg atgaaccgag ccagggctgg accggcggtt atgccaccct ggggagtacc
     3481 ggggcggtgt atttgacctg cgagtttgtg gtgttggacg gtgaatttgc ccgccgcaag
     3541 ctctggtcgc gcatcggcct gtatagcccc aaaggtcccg agtggagtaa caagggccgc
     3601 acctttatca aagccctgct caattcggcg cgcggtattt ggcccagcga caccagtccg
     3661 gccgcccagg aggcgcggcg cattgccagt tttgcggagc ttgacggcat agtgtttatc
     3721 ggccgggtcg actgggaaag gaataaaaac ggcgaattcg acaacgtcat caaaaccgcc
     3781 attacaccgg accatgcgga gtacgccgca cgggtggatg cnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn ntgcagcggc tagtggccgc tcacaatcca gccaaggtcg
     3961 ccccagttgg gcgcagggcg gaggagacac gccatgacgc ttaagcctcc cagcatccct
     4021 tggtgcgatg tatttggcac tcaagatgta ctcatggcaa ccggcctgca cgcctggatc
     4081 tgtgtgttga tgatgcgcgg ccgctggcat gcggtgcgcg gtgatgatcg tcatcccatc
     4141 cagcggctcg ccctcggcga ccgtcaaaat tgtctggccg ttgccgagct ctggttccag
     4201 caggtcgcac ccaccgacct cgggattatc gagcattgga tggcgcgtcc cgcatcgcct
     4261 aagcaactta cgcggctgtc gcccctgctg cagaccgaca cacccatgac ccgctatcag
     4321 gcgtcggccc ggctgcagtt tcagtacaac gccgccaccc tagtgcgcct ggtcaacacc
     4381 gtcgcttccg ttgccgggga ggttctatga cctgtgccct ttgctatgga ccttcgaatg
     4441 ggtttggctg gatggaaccg cgccgtccca tgacgccgat tcgcaagcgt tggctgtttt
     4501 gctcgcgtcg ttgcctggag gtgtttatca cccttcgttt tggggagcct accatgctcg
     4561 atccagtggt catgaacacc acggccttga aggcctgcct aaatcccctc gggcagtatc
     4621 tggacgccgc agggctcaac cgccccctgg cggactatag ccaggacgag gttctggcgc
     4681 tggtcgatac cctcgtggtg gcctaccggg attccatgtt gagccaatac gaagcggcct
     4741 tagtgcaaga acgtctgctc tacgagcatc cccaaaaacg gcttgattcc cctcctgtga
     4801 gtcgaggagt gcgcgcatga gtgagtacac cccctcagac gaacagccgt cgtggacgga
     4861 ttttaatgat gcgccctccg gggacagcac agaccgcttc actgctgagg atacggatgc
     4921 tctgcgcggt gcgctggtgg atcgtctgga cgatatcctg gggtacctgt tgccccaggg
     4981 ccgcgcacgc ggtgaaaagt attacgttgg cgatctcgac ggcaacgccg gtaaaagcct
     5041 ggtagtagag ctggccggac ccaaacgcgg cctctggcag gactttgcca ccggtgaagg
     5101 cggtgatcta tttgacctct gggccgccgt tcgaggcctg tccgcccgtg cgcattttcc
     5161 tgagctggtg gccgagattc gccaatggct ggggcttgtg gcgcacacgc ctgtcagcga
     5221 tctgccaggg cgttcagcac tgatcccatc gactgtatcg cccgccaccg ctacctctgc
     5281 ggccttgagc tctggcccat tgggcctgcc cttagcgcat tccacgcccc gccctaaagt
     5341 ccgtgtaccc gtgttggatg aactcggccc ctataccgcc aaatgggact atcaaaccgc
     5401 ccagggcgag ttgatcgcct gtgtctaccg ctatgatccc ccggacggta aggaatttag
     5461 gccctgggat gtacgcgcgc gtctgtggcg tacaccggac ccgcgtccgc tgtacaacca
     5521 acccgccctg agtagcgcta agcatgtgat tctggtggaa ggcgagaagt gtgcggaggc
     5581 gctgatccgt gcgggcctgg tcgccaccac agccatgaat ggcgcccatg ctccggtgga
     5641 taaaaccgat tggcggccgc ttaagggcaa gcatgtgttg atctggccgg atcgcgatgc
     5701 acctggatgg gattatgcgg agagcgcagc gcgggcctgt gtcctggtcg gctgtgcctc
     5761 ggtggcgatt ctggtgccgc ccaccgataa accggagaag tgggatgcgg ccgatgccct
     5821 ggctgaag
//
LOCUS       DAOQGS010000237         5826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6906_length_5826_cov_4.915786, whole genome shotgun sequence.
ACCESSION   DAOQGS010000237 DAOQGS010000000
VERSION     DAOQGS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6906_length_5826_cov_4.915786"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2221
                     /gene="metE"
                     /locus_tag="PKZ52_09945"
     CDS             <1..2221
                     /gene="metE"
                     /locus_tag="PKZ52_09945"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNG60138.1"
                     /translation="NESYWRGELTELNYLARTEAIQQQNFQHQLASGLNYLPVGDFSF
                     YDHVLDTSFWLGNLPARSGQGNSIDNYFRAARGRGACGDGCIAGEMTKWFDTNYHYIV
                     PEFTAHTQFSLQLDFWRQHFAAVKNQAHASKPVILGPLSYLWLGKTKDGSEKLHLLER
                     LIPVYRDLLGEFAAQGFNWVQIDEPILALDLPDAWLNAFEQAYHRLQTAGIKSLVTVY
                     FGPLQRNLRTALALPVAGVHLDMLRGKEDLTAALDLLPGHKILSLGVIDGRNIWCSDL
                     TQLLNWLEPVAARLNERLWLAPSCSLLHVPVDLNCEQQLPAEIKTWLSFAAQKLEELN
                     IVKIALNQGRDAVAAALAENKHALDRRGQSASVHKPHIKQAVSESVQQGVDRPAPFAL
                     RANLQQQKLQLPLFPTTSIGSFPQTQEIRAARKAFKTGAINTEQYNACIAEHIIKAIQ
                     VQEELGMDVLVHGEAERNDMVEYFAEFLQGVCFSEYGWVQSYGSRCVKPPIIYGDIER
                     RSPMTVYWSEFAASNTHKPMKGMLTGPITLLQWSFVRDDQPRAQTALQLALALRAEVQ
                     DLERAGINIIQVDEPALREGLPLRKAHWDEYLNWAVQAFKLCVSAVTNDTQIHTHMCY
                     AEFNDILPAIAALDADVITIETSRSDMELLSAFSEFAYPNAIGPGVYDIHSPNLPSQT
                     HMENLLRKALETIPAERLWVNPDCGLKTRGWPETLAALQTMVAAAKQLRSECATAIN"
     gene            2501..5023
                     /locus_tag="PKZ52_09950"
     CDS             2501..5023
                     /locus_tag="PKZ52_09950"
                     /inference="COORDINATES: protein motif:HMM:NF037461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNG60139.1"
                     /translation="MQRLRCAFVGALLLALYGCGGGAGKAPQSSSSSSSSSGKTSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSGAIAFNCAQNSCDIFTDQLVSGRGVLAAF
                     EWLATNTGGYQYESNKNSHVQWSMVNLQDAAHKNVIEVTFNANDPNELVNDNGWFGLA
                     AGTTEAATMDLSAFAAGSVSFDLRIIKNGQVADNLDFKMECIYPCTSEEFWVADPAGL
                     NEWKTYTLPIARMIETGLDIKRVNNLFILKPWWGNQRGQYVVQLDNIRLNKQFTPVPF
                     VPPAAPTTSISRSYYQNGASADSYFTIGNSLDGLSARYSEVIDGTNKVMDVTTLVENF
                     PIYLVSYLNTGAQYDLADYYYGDVVFDLKIVDYAGATGNFEFVANCGGRCRNLPNVNL
                     GHPAEGIWHTLRIPVKNLVANGLELSRIHSAFNLQLFGQSKAGLHVQVNNVRWEYTAS
                     GSSSSSSSSSSSGQPVAFNCTADICDLFKDRAEPGHGVLADFEWKASGTGVYHYQSAA
                     DAHVQWQLVNSQDSAHGTVVEVTYNANDPSDAVNDNGWFGLAVGNAENTTADLSAYAD
                     GAISFDMRVIRNGQITDNFDVKMECIYPCTSEEFWVPDGANNGQWQTHTLPIKRMIET
                     GLDIKKVNNLIIIKPGWGNQRGQYVVQLDNIRLLKNYVAPSVTSPVKPAENKTLYYFQ
                     NAVAAGSRVYIANTADGLGASFNQVVDGSSTVIDLVTNISNMGLFLFASGEFYQRQNL
                     TDFYYGNVVFDLRVLNYSGNTSALSFTVNCGGRCRNVPSFTIGHPEAGSWITYRIPVK
                     TLADNGVDLTRVTDSFMLTLTGSSQPGVHLQVNNIRWEVLSP"
     gene            complement(5134..>5826)
                     /locus_tag="PKZ52_09955"
     CDS             complement(5134..>5826)
                     /locus_tag="PKZ52_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HNG60140.1"
                     /translation="ASCRRQALLGYFGDELKEPCGNCDNCLAPPLSWDATEPAQKVLS
                     TIYRSGQRFGSQHIIDIVRGEATEKIKQYQHQELSTFGIGKNLSETQWRSVLRQLTAR
                     GFIDVDTEGFGSLRLNDSCRPILRGESTLLLRQELPSAKTEKSGTGKSKGEEVPSHLL
                     PFWYALRACRKRLADSAGVPPYVVFHDSTLKEVMRKKPQNLRQLGLVSGVGDNKLKKY
                     GEEFLAVVKEFT"
BASE COUNT         1445 a         1355 c         1502 g         1524 t
ORIGIN      
        1 caatgaatcc tactggcgcg gtgaacttac tgagttgaat tatttagcgc gcacagaggc
       61 catccagcaa caaaatttcc aacaccaatt ggccagtggc ctgaattatc tccctgtagg
      121 ggatttctcc ttttatgacc acgtgttgga tacgagcttt tggctgggta atttgcccgc
      181 ccgcagcggc cagggtaatt caatcgacaa ttattttcgc gcagcgcggg gccgaggtgc
      241 ctgcggcgac ggctgtattg caggggaaat gaccaagtgg tttgatacca attaccacta
      301 tatagtgcct gaatttactg cgcacacgca gttttcgttg caattggatt tttggcggca
      361 acattttgcg gcggttaaaa accaggctca tgcgagtaaa ccggtgattc tggggccatt
      421 gagttatttg tggctgggta aaactaaaga tggcagtgag aaacttcatt tgcttgagcg
      481 gttaattccg gtttaccgcg atttattggg cgagtttgcg gcgcagggtt ttaattgggt
      541 gcagattgac gagcctatac tcgcgctgga tttgcccgat gcctggttaa atgcctttga
      601 gcaggcctac catcgcctac aaacggcggg tataaaaagc cttgtaacgg tatatttcgg
      661 cccgctgcag cgcaatttgc gcacggcttt ggcgctgccc gtggcgggtg tgcatttgga
      721 tatgctgcgc ggcaaagagg atttaaccgc ggcgcttgat ttattgcccg gccataaaat
      781 tctctcgctg ggggtaattg atggccgcaa tatttggtgc agtgacttaa cgcaattatt
      841 aaattggtta gaacctgtag ctgcgcgttt aaatgagcgt ctgtggctgg caccttcttg
      901 ctcgttgtta catgttcctg ttgatctaaa ttgcgagcag caattgccag ctgagattaa
      961 aacttggttg agttttgctg cgcaaaaatt agaagaacta aatattgtga agatcgccct
     1021 taaccagggg cgcgatgcgg tggcggcggc gcttgctgaa aataaacatg cccttgatcg
     1081 gcgggggcaa tccgcaagcg ttcataaacc gcatatcaaa caggccgtga gcgaaagtgt
     1141 gcagcagggt gttgataggc ctgcgccatt tgccctgcgt gccaatcttc agcaacaaaa
     1201 gctgcaattg ccgttatttc caaccaccag catcggctcg tttccgcaga ctcaagaaat
     1261 tcgtgcagcg cgcaaagcat ttaaaactgg ggctataaat accgagcaat acaatgcctg
     1321 tattgccgag cacatcatta aagccattca ggttcaggag gaattgggaa tggatgtgct
     1381 ggtgcacggg gaggcagagc gcaatgacat ggtggaatat ttcgccgaat ttttgcaagg
     1441 cgtgtgtttt agcgaatatg gctgggtgca aagttacggt agccgctgtg taaaaccgcc
     1501 cattatttac ggcgatatag aacgcaggtc gcccatgacg gtgtattgga gtgagtttgc
     1561 cgctagcaac acacacaaac ctatgaaggg aatgttaacc gggccgatta ccttgttgca
     1621 gtggtcgttt gtgcgcgatg accaaccccg cgcgcaaacc gcactgcaat tagccctagc
     1681 attgcgagca gaagtgcaag acctcgagcg tgcaggaatt aatattattc aggtggatga
     1741 acccgccctg cgggagggat taccgctgcg caaagcccat tgggatgagt atttaaattg
     1801 ggccgtgcag gcctttaaat tatgcgtaag cgccgtgaca aacgatacac aaattcacac
     1861 ccatatgtgt tacgccgaat ttaacgatat tttaccggcc atagctgcgt tggatgccga
     1921 tgtcatcacc attgaaacct cccgctcgga tatggaatta ttaagcgcat ttagtgaatt
     1981 cgcctacccc aacgccatag gcccaggtgt gtacgatatc cacagcccca atttgccaag
     2041 tcaaacccat atggaaaatt tattgcgtaa ggctttggaa actatccctg ccgagcgcct
     2101 gtgggtaaac cctgactgcg gtttaaaaac ccgcggctgg ccggagacac tggcagcatt
     2161 gcagaccatg gtagcagcgg cgaagcagct gcgctctgag tgtgcaaccg caattaatta
     2221 aatgctccca ggtaaaaagc gtttttaacc ggctgctaga tttcgccaaa aatctgcaaa
     2281 gatagaatat tgccaggagt gttttattaa atattacaga atacggagta agaaatagtg
     2341 cacaaccagc aattgttgct cgtgtaaccg ggggcacggt tgggtattct cgcagccggg
     2401 gcatcgtggc cggtggaatt ttccattaca ctgcgcgccc cggcgctgcg gcagggccgc
     2461 aacgcgccaa attaccctac tatttctacc aggagctttt atgcagcgat tgagatgtgc
     2521 ctttgtcggg gcgcttttgt tggccctata cggttgtgga ggcggcgcag gtaaagcgcc
     2581 tcagtcatca tccagcagtt catcctcgtc aggcaaaacc tcgtcttcca gtagctcttc
     2641 atccagctcg tcatcatcaa gtagctcctc atctagcagc tcaagttcat cctcaagcag
     2701 tagctcgtcc tcaagctcgg gtgctattgc atttaattgc gcacaaaaca gctgtgatat
     2761 ttttaccgat cagctggtat cgggccgtgg tgtgttggcg gcttttgagt ggttagcgac
     2821 caatactggt ggttatcagt acgagagcaa taaaaattcc cacgtacagt ggagcatggt
     2881 taatctgcag gatgcggctc acaaaaatgt cattgaagtg acctttaacg ccaatgaccc
     2941 caacgagttg gtaaatgaca atggttggtt tggcctggcg gccggtacca ctgaagcagc
     3001 cactatggat ttaagcgcat ttgccgccgg ttccgtcagt tttgatctgc gcattatcaa
     3061 aaatggccaa gtggcggata acctagattt taaaatggag tgtatttacc cctgcacctc
     3121 cgaagaattt tgggtggctg atcctgccgg tcttaatgag tggaaaacct atacgctacc
     3181 cattgcccgc atgattgaaa ccgggctgga tattaagcgt gtcaataatc tgtttatctt
     3241 aaaaccctgg tggggcaacc agcgcggcca atatgtggtg cagctggata atattcgttt
     3301 gaataaacag tttacccctg tgccgtttgt gccacctgca gcgccaacta ccagcatcag
     3361 tcgcagttat tatcaaaatg gcgccagtgc agatagctat tttacgatcg gtaattccct
     3421 agatggtttg tccgctcgtt atagcgaagt aattgacggc acaaataagg taatggatgt
     3481 gaccactttg gtggaaaact tccctattta tttggtctcc tatttgaata cgggtgcgca
     3541 atacgatttg gctgattatt actacggcga tgtggttttt gatttaaaaa ttgttgatta
     3601 tgcaggcgca actggcaatt ttgagtttgt agccaattgc ggtggccgct gccgcaacct
     3661 gccaaatgtt aatttggggc atccggctga aggaatttgg cataccctac gcattcctgt
     3721 aaaaaacctg gtggccaatg gattggaatt atcccgtatc cacagtgcct ttaatttgca
     3781 attgttcggt cagagtaaag ctggcttgca tgtgcaggtg aataatgtgc gctgggaata
     3841 caccgcaagc ggtagcagca gttcaagcag ctcaagttct tcgagcgggc agcctgtggc
     3901 atttaattgt acggcggata tttgcgattt atttaaagat cgagcagaac ccggccacgg
     3961 cgttttagcg gattttgaat ggaaggcgag tggcacgggt gtttatcact accaaagtgc
     4021 agcggatgcc catgtgcaat ggcagttggt taatagccaa gattccgccc acggtacagt
     4081 tgttgaagtg acctacaacg ccaacgaccc aagcgatgca gttaatgaca atggctggtt
     4141 tggcctggcg gttggtaatg ctgaaaatac cacggcggat ttaagcgcct atgccgatgg
     4201 cgccatcagt tttgatatgc gcgttatccg caatggccag attaccgata actttgatgt
     4261 aaaaatggaa tgtatatacc cctgtacctc tgaggaattt tgggtgccgg atggagcgaa
     4321 taacgggcaa tggcaaaccc atacgctgcc cataaaacgc atgatcgaaa ccggcttgga
     4381 tattaaaaag gtcaataatt taattatcat caagccgggc tggggcaacc agcggggcca
     4441 atatgtagtg cagctggata atattcgtct gcttaaaaac tatgtggcgc ccagtgtaac
     4501 ctcacctgtt aagcctgctg aaaataaaac actctactat tttcaaaatg cagttgcggc
     4561 cggtagccgc gtttatattg ccaacacggc ggatgggtta ggggctagtt ttaatcaggt
     4621 tgttgatggc agctcaacgg tgattgacct ggtgactaac atcagcaata tgggcctttt
     4681 tctgtttgcc agtggtgaat tctaccagcg ccaaaacctt acggactttt attatggcaa
     4741 tgtggtattt gatctgcgcg tgctcaatta ttcaggcaat acaagtgcct taagcttcac
     4801 cgttaactgc ggtggccgct gccgcaatgt gccgagtttt accattggcc acccggaggc
     4861 aggcagttgg attacctata gaattccggt taaaacactg gcggataatg gtgtagattt
     4921 aacgcgggta acggatagct ttatgcttac gctaacaggc tcaagtcagc caggtgtaca
     4981 tctgcaagta aataatattc gctgggaagt gctctcaccc taaaggagtt tagttaaacc
     5041 ctgtcccata aaaaagccga gactattctc ggctttttta tctctcactc ccaggctctg
     5101 cgtgggagtg agagaaataa atatacatca ggcttaagtg aattccttca ccaccgcaag
     5161 aaactcctcg ccgtattttt ttaatttatt atcccccaca ccactcacca accccagttg
     5221 ccgcaaattc tgcggttttt tgcgcatcac ttcttttagc gtactatcgt ggaatactac
     5281 ataaggcggc acgcctgcgc tgtcggccag gcgtttgcgg caggcgcgca gggcgtacca
     5341 aaagggcaat aaatggctgg gcacttcttc gcccttggat ttgcctgtac cggatttctc
     5401 cgttttggcg ctgggtaatt cctgtcgcag caataaagta ctttcaccgc gcaaaatagg
     5461 ccgacagctg tcgtttaggc gcaggctgcc aaaaccttct gtgtccacat cgataaaacc
     5521 tcgcgccgtt aattgccgca gtaccgagcg ccactgggtt tcgctaaggt ttttgccaat
     5581 gccgaaggtg gataattcct ggtgttgata ttgtttaatt ttttctgtgg cttcgccgcg
     5641 cacaatatca ataatgtgtt gagagccaaa acgctggccg ctgcggtaaa tggtggaaag
     5701 caccttttgc gcaggctcgg tggcatccca acttaaaggc ggcgctaaac aattatcgca
     5761 attgccgcag ggctctttta attcatcgcc aaaataaccc agcagggctt ggcggcggca
     5821 gctggc
//
LOCUS       DAOQGS010000238         5809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6937_length_5809_cov_5.241050, whole genome shotgun sequence.
ACCESSION   DAOQGS010000238 DAOQGS010000000
VERSION     DAOQGS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6937_length_5809_cov_5.241050"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PKZ52_09960"
     CDS             <1..1416
                     /locus_tag="PKZ52_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNG60141.1"
                     /translation="VRQNGALVESLAIGYRDRTLNENMPKEPILRLASVDKSPTRAAI
                     RKVIELGTSLTKNPAYTGPYPITPNTKVFGPGGILSDITPVGGVLGTNVEKITVAHLH
                     DHISGINDNGRDEQAWEDLLAYQYGIPASQVTAKQLIGYWISQPTIATPGQDNPGSHY
                     SSGGHAVLRYIVERVSGMTIADFLKTKLNATDFAVAYERMEGRKLGVNERETGYGLFG
                     KETRSRWFELEEYRALSASAEGLTKFFYDNDLGNKDFSYKDNNVFLGPNGGGAMDGTR
                     SGAVIENNSAGKIAYAYIWNSDIPPSKRFEIKRTLALYDDQFPGTCNPTDTTIAQGQS
                     FRIDSVASKGWTMHTGNSNDVNDPSYTTTPQISAVGGTPDVWGFQGVNWRFEKVAGQA
                     YYVIRNNLKNKFLGVTSSQPVLKDQAITDDTMWYLRDMTNHFRLENRSGQTLYVDSAN
                     ILRTGTYDGTTADKFKWYICN"
     gene            complement(1527..4043)
                     /locus_tag="PKZ52_09965"
     CDS             complement(1527..4043)
                     /locus_tag="PKZ52_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG60142.1"
                     /translation="MPIRIFKSRYAYSKWILLLACLGSARIALSDSGASEGHYFDIPQ
                     LPLHQSVLEFAIQADCEVIAQEQDLSRLQGRSVRGFHPPLQALQELLADANLTVEFFT
                     AAKAYVIRAAPVPSTSPTAAQVHVIEEILVTGQRYPVRYQTVVNTEDRYGGAMFDSTR
                     AHNIVPAAVLEDSASDNLMEALRYVSSATPGDGFADSNDDYFIRGFSRQNTYINGLRL
                     SNSTAIQVVPDTVKRLDVLKGPSMLFYGQSSAGGVVDITRKQPVPEDRLRMDLTLGEP
                     SRHKLFVEANKGQLLGNMDFLLMGMDDKDQESADGQERHRQMVNLSGQGSLENQLVYS
                     AGYEYQFLNKASALDLPVFSDSNQFLPYRGRDFLNQADNDFSASAELFDGSLSYTLMP
                     EWLIQGNMLWQREYRDGVRTGDNFLTNSHIFLDPNSALPRVGIAAIMGQVAAPIAQLG
                     PNYTFGLVESIYDQHETEHAFTTSLALNGQLQTGAVEHKLIVGADIYNQSLHQEFAIE
                     KRMFAPRPVFSAAILQTPQQTLIDMMLREPPASRGINLQALEVARTDWGSYFQIRSSW
                     TTDWSTSIGWRYSHFSETRREIDGGSPDLEGSYDDWLLQVGTSWLISDTLSFYSNYSE
                     TLNLNYLVDDSNHFVEQPERSQQQELGFRWQAPDGQLLSTLSLFDITSSGINSVKFDA
                     GYRRLQPPQQHKVQGIELDLTWRMNEQVEWITSVGLMRNEVNKQNASSGYPSMVADNT
                     LGILGRISLTESWAYYAGFNYVSDRSIGELGSAKLGEYTLVDMSLEKTLKSTAHEWRI
                     RVIGKNLLDEYHPSVAIPGVRVNPTSGRQALVKFTYEFQK"
     gene            complement(4024..4992)
                     /locus_tag="PKZ52_09970"
     CDS             complement(4024..4992)
                     /locus_tag="PKZ52_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNG60143.1"
                     /translation="MKYLLVAYLTLFSWMLASCESADRGGVKLTQKPSPPSIQPPQVT
                     NNEPVSIISATAKCLAVYEPDFHNASDGSKVQTVDCVPGSIYQNWLFTPQREILSDNG
                     QCLAPAGKPEAGTLVVLNHCDGSVDQKWVLDVQSISIRLGNISDLCLEMPKLTIQFNG
                     SQLVLEPCHGEDNQQWVAQKLLHKQLISALYPGLSISLEPSAASGPPRLAVTPSVVDW
                     TSAAWTLEPIGNFYRIRSQWRPDRFLHLERGFLEAGTISPYWDSAIWMIIRKDDSQGE
                     AYLLRNLRLNNIYIGLDADQVLKAGTLSELADGSSLWRVIDANQDF"
     gene            complement(4995..>5809)
                     /locus_tag="PKZ52_09975"
     CDS             complement(4995..>5809)
                     /locus_tag="PKZ52_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNG60144.1"
                     /translation="GTAQLRVSKKAVVPFNHFGFWVSAVAAVLVAAVLVYLSPFQIEA
                     VPEFEKYVALTEQKTVTLTDQSSIVLSPNSQIEVQYSQSARELHLHKGQIFLSVTKDA
                     QRPFKVLTHQGAVRVMGTQFTVQQLAEDLKITVVEGVVGLLTSERAKLDQTPFVVLHK
                     NQQILYSDALKGAPPTAVDAARETSWSKGRMIFDGDALGEVTVALNEHLSPQIQIASP
                     ELRNKRIVGAISVKNSRVAAASLATIVGAVVEETPNQDALLLKSVSDESSEK"
BASE COUNT         1517 a         1446 c         1389 g         1457 t
ORIGIN      
        1 gttcggcaaa acggcgcgct tgttgagtcc ttggctatag gttacagaga ccggacgttg
       61 aatgaaaata tgccaaagga acccattctt cgcttagcca gtgtcgataa gtcaccaacc
      121 cgcgcggcca ttcgcaaggt aatagagctg ggcacatcgc ttaccaaaaa tcctgcctac
      181 acaggtcctt atcccattac ccctaataca aaagtatttg ggccaggggg aattttaagt
      241 gatattactc ccgttggcgg cgtgctaggg acaaatgtcg agaaaataac ggttgcgcat
      301 cttcatgatc atattagcgg tattaatgat aatggtcgtg acgaacaggc ctgggaagac
      361 ttgcttgcat accagtatgg gattcctgcc tcgcaagtta ctgccaaaca gctgattggg
      421 tactggataa gtcagccgac aattgcgacg cccggacaag ataatcccgg cagtcattac
      481 agtagtggcg ggcacgcggt tttacgctac attgttgagc gggtttcagg aatgacgatt
      541 gctgacttcc ttaaaaccaa gcttaatgca acagactttg ctgtagccta cgagcgcatg
      601 gaaggcagaa aactgggtgt caatgagcgt gagacgggat atggattatt cggcaaggaa
      661 actcgttcac gctggtttga gcttgaagaa tatcgcgcgc taagtgcatc ggcagaaggg
      721 ttaacaaaat tcttttacga taacgattta ggcaataaag atttttctta caaggataat
      781 aatgtgtttt taggcccgaa tggcggcggc gcaatggacg gtactcgctc tggcgcagta
      841 attgaaaata atagcgcagg caaaatcgct tatgcttaca tttggaacag tgacataccg
      901 cctagcaaac gattcgagat taaacggaca cttgctcttt atgacgatca gtttccaggg
      961 acatgtaacc ctaccgatac aaccatcgcc caaggacaga gcttccgcat cgacagcgtg
     1021 gccagcaaag gatggaccat gcatactggc aatagtaatg atgtaaatga cccgtcttat
     1081 acaactacgc cacaaatctc tgctgtgggc ggcactccag atgtatgggg ctttcagggc
     1141 gttaactgga gatttgaaaa ggttgccgga caggcctact acgtaattcg caacaactta
     1201 aaaaataaat ttttgggtgt aacgagttct caacctgttc taaaagatca agcaatcact
     1261 gacgatacaa tgtggtactt gcgcgatatg accaatcact ttcgattaga aaatcgcagc
     1321 gggcaaacac tctatgttga ttcagcgaat attctccgaa cggggacata tgatgggaca
     1381 actgcagata aatttaagtg gtatatctgc aattaattgg gagacttgta gcaaggatga
     1441 atagttaatt acgggagatt tacttacagg agttgcgact aggggcgcac ttttgaatag
     1501 cccctagtcg ttttaatgaa gcgcaattat ttttgaaatt cataggtgaa tttcacaagc
     1561 gcctgtcggc cagaagtggg attgacgcga acaccgggta tggcaaccga cgggtgatat
     1621 tcgtccagaa ggttttttcc tatcacgcga atccgccatt catgtgcagt ggatttcaga
     1681 gttttctcaa gcgacatatc gacgagtgta tattcaccga gctttgcact gcctaattcg
     1741 ccgatggatc tatcactaac gtaattaaag ccggcatagt aggcccagct ttcagtaagg
     1801 ctgattcggc cgagaattcc cagagtgtta tcagcgacca tgctgggata tcccgatgat
     1861 gcattttgtt tattaacttc attgcgcatc aaaccgacac tggtgatcca ctcaacctgc
     1921 tcattcatcc gccaggttaa atcaagctct atcccttgaa ccttgtgttg ttgtggtggc
     1981 tgtaaccttc gataaccagc atcaaatttt acagagttga ttcccgagct tgtaatgtca
     2041 aatagtgaaa gcgtgctgag caattgccca tctggcgcct gccacctaaa tcccaattct
     2101 tgttgttgtg accgttcagg ctgctctaca aaatggttgg aatcatcaac cagataattt
     2161 aagttcagtg tttccgaata attactgtaa aaagaaagtg tgtcggatat tagccagctg
     2221 gtgccaacct gcagcaacca atcatcataa ctgccttcca gatccgggct gccgccgtcg
     2281 atttcccgcc gcgtctcact aaaatgcgaa taacgccaac cgatgcttgt gctccaatcc
     2341 gttgtccaac tcgatcggat ctgaaaatag ctaccccagt cagtgcgtgc aacctccaag
     2401 gcttgcaaat taataccccg gctcgcaggc ggctctcgca gcatcatatc gattaatgtc
     2461 tgttgcggtg tttgcaggat ggccgcgcta aaaacaggtc ttggagcaaa catcctcttt
     2521 tctatcgcaa actcctgatg taatgactga ttataaatat cggccccgac aattagcttg
     2581 tgctcaaccg cccccgtttg gagctgcccg ttaagtgcca agcttgttgt gaacgcatgt
     2641 tcagtttcgt gttgatcgta aatactctcc actaaaccaa acgtgtaatt agggcccaac
     2701 tgagcaatag gcgctgccac ctgccccata atcgcggcaa tgcctactct aggtagggca
     2761 gaatttggat cgaggaagat gtgggaattg gtgagaaaat tatctcctgt tcgtactcca
     2821 tcgcgatatt cccgctgcca gagcatattt ccctggatta gccattcagg catcagcgta
     2881 taactcagtg aaccatcaaa taattcagct gaggctgaaa agtcattatc tgcctgattg
     2941 agaaaatcgc ggccccgata gggcaaaaac tgattggaat cggaaaaaac aggcaaatct
     3001 agtgcgcttg ccttattcaa aaactgatat tcatagcctg cgctataaac tagctgattc
     3061 tccaaactgc cttgtccact tagattaacc atttgacgat gacgctcctg gccatctgca
     3121 ctttcttggt ccttatcgtc catccccatc aacagaaaat ccatattgcc aagcagctgg
     3181 cctttgttag cctcgacgaa cagcttgtgc cgcgagggct ccccgagcgt aagatccata
     3241 cgcagtcgat cttccggcac tggctgcttg cgggtgatat caactactcc acctgcacta
     3301 gactgcccgt agaacaacat tgacggacct ttcaaaacat ccaagcgttt tacagtgtct
     3361 ggcacaacct gtatggcagt gctgttgctt agccggagcc cattgatata ggtattctgc
     3421 cgcgaaaaac cgcgaatgaa ataatcgtcg ttgctgtcag cgaaaccgtc accgggcgtc
     3481 gctgagctta cgtacctcag cgcttccatc aaattatcgc tagccgagtc ctccaacaca
     3541 gcggccggca ctatattgtg ggcacgggtg ctatcgaaca tagctccacc atagcgatcc
     3601 tcggtgttga ccacggtttg atagcgcacc ggatatcgtt gcccagtcac aaggatttct
     3661 tcgatgacat gcacttgggc agcggttggt gaggtcgatg gcacgggagc tgcgcgaata
     3721 acgtacgctt tggctgcagt gaaaaattcc accgttagat tggcatcagc cagcagctct
     3781 tggagggcct gcaaaggcgg atggaacccg cgcacactgc ggccctgcaa tcggcttaag
     3841 tcctgctctt gagcaatgac ttcacaatcg gcctgaatcg cgaattccaa tacgctctga
     3901 tgcaggggga gttgtggaat gtcgaaataa tgtccctcgc tagcgcctga gtccgacaac
     3961 gcaatgcgcg cggaccccaa acaagcaagc aacagaatcc atttggagta agcgtagcgt
     4021 gatttaaaaa tcctgattgg catctatcac tcgccaaaga ctgctgccgt cagccaattc
     4081 tgacaaggtg cccgccttaa gtacctgatc tgcatccagt cctatgtaga tattattgag
     4141 cctgaggttg cgcaagagat aggcctcacc ttggctgtcg tctttgcgaa taatcatcca
     4201 gatagcactg tcccaatagg ggcttatggt gccggcctct aaaaaccccc gctcaaggtg
     4261 taggaaacga tctggtcgcc actggctgcg gattctataa aaattcccga tgggctctaa
     4321 agtccacgct gcactggtcc aatctacaac gctaggtgtt acggccaggc gtggcgggcc
     4381 actcgccgca ctcggctcaa gactgatcga gagccctgga tacaacgcac taatcagctg
     4441 cttgtgaaga agtttctggg ccacccactg ctggttatct tcaccgtgac aaggctccag
     4501 cacgagttgg ctaccattga attgaatagt gagcttaggc atttccaagc agagatcgct
     4561 gatatttcca aggcgtattg atatggactg cacgtctaac acccacttct gatctacgga
     4621 gccgtcgcag tgattgagca caaccaacgt accagcctcc ggttttcccg ctggcgccag
     4681 acactggcca ttgtccgaga ggatttctcg ctgcggcgta aagagccagt tttgatagat
     4741 agagccggga acacaatcga ccgtctgcac tttagatccg tcgctggcat tgtggaaatc
     4801 aggttcatat accgccaaac acttggcagt ggcagaaata atggagacgg gttcgttgtt
     4861 agttacttgc ggcggctgaa tgctgggcgg agacggcttc tgagttaatt tcacgccgcc
     4921 gcgatcggcg ctttcgcagg atgcaagcat ccagctgaaa agggttagat atgctaccaa
     4981 taaatacttc atacttattt ttctgaggat tcatctgaaa ctgatttgag cagtagagca
     5041 tcttgattcg gtgtttcctc aactacagcg ccgacaatgg ttgcgagact tgcagcggcc
     5101 acccgtgaat tcttcacaga tatagcaccc actatgcgct tgttgcgaag ttctgggcta
     5161 gcaatttgaa tctgcgggga caggtgttca ttgagggcaa ccgttacctc acccagagca
     5221 tcgccatcaa aaatcatacg gcccttagac cagctggttt ctctagcagc atcaacagcg
     5281 gttggcggcg ccccttttaa ggcatctgaa tataaaattt gttgattctt gtgcagcacc
     5341 acgaaaggtg tttgatctaa cttcgcgcgc tctgatgtca gcaaccccac cacgccctcg
     5401 acgactgtaa ttttcaaatc ctccgccaac tgctgcaccg taaattgagt ccccatcacg
     5461 cgcacagcac cttggtgagt cagcacctta aatggcctct gtgcatcctt ggttacactc
     5521 agaaatattt ggcctttgtg cagatgaagt tcgcgagcgg attgggagta ctgaacttcg
     5581 atctggctgt tagggctcaa tacaatggac gattgatcgg tcaaagttac ggttttttgc
     5641 tcagttagcg cgacgtattt ttcaaactca ggaactgctt ctatctgaaa gggcgataag
     5701 taaaccaata cagctgcaac caagaccgca gctaccgcgc tcacccaaaa accgaaatgg
     5761 ttaaagggca ctaccgcttt tttagatacc cgtaactgag cagtgccgc
//
LOCUS       DAOQGS010000239         5798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6959_length_5798_cov_4.135818, whole genome shotgun sequence.
ACCESSION   DAOQGS010000239 DAOQGS010000000
VERSION     DAOQGS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_5798_cov_4.135818"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2562
                     /locus_tag="PKZ52_09980"
     CDS             <1..2562
                     /locus_tag="PKZ52_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNG60145.1"
                     /translation="IDRGSCMFRPLGISCLALAMLACSQHSTQTTPTANPAPVQPDKR
                     ALWPQPKSPFSAAETAAVNARVAQLLAGMSLEEKVGQMVVAEMGFITPEQVKEFSVGA
                     VLEAGGAYLNKNKQAKISDWVLQTQRYYVASVDKSGGRAGIPILWGTDALHGHNKAFG
                     ATIFPHNIGLGASRNGDLVRRIAEVTAVETLIAGKNWTYAPVVSVVRDDRWGRSYESY
                     SENSALVADLGRQAVLGLQGEVGTTFMQPNRLMATAKHFIGDGGTEDGVDRANNLASE
                     EDLIRIHSPGYVSTLEAGVLSVMASHNSWQGTRLHGHKYLLTTILKEHMGFDGFVVGD
                     WNSHGLIPGCTNSDCPDSINAGVDMIMVPEHWKEFIHTTIGHVKSGRISQARVDDAVS
                     RILRSKIRAGLMESGEPIKRPYSNQTAQFTNPEHHALARQAVRESLVLLKNNGQLLPL
                     NPKQNILITGEGADNIPQACGGWTISWQGTGLDNNDFPNATSIAQGLKQQIQAAGGKA
                     IISKDGSFKGKPAVAVVVFGEKPYAEWQGDVKTLAYQPRVNSDAKLLESLKAQGIKTV
                     AVFLSGRPMWVNRELNASDAFVAAWLPGTEGGGIADVLLRKKDGTIAHDFKGKLSFSW
                     PAKPDQTSNEGDGSTPLFPYGFGLSYAAPLADLGPLSTDVGGAADLINGNTPMFNGRA
                     KAPWVLNIQSASEVQPYQSSTLAGKVITLTEADQHTQGDAVDAQWNGTGAGLLALQTP
                     KASQNTTDLLARKGVVAFDLMVLQKPTAKVEFEMSYAPKSIAHREDFTAQLGSAAPNT
                     WRRYRVDMACFAKQKIDFSELNQGFGLVTSGKLQVRLANVVMEPDEAEQADIKCQ"
     gene            2974..4518
                     /locus_tag="PKZ52_09985"
     CDS             2974..4518
                     /locus_tag="PKZ52_09985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNG60146.1"
                     /translation="MKQSLQLKLGQSLTMTPQLQQAIRLLQLSTLDLQAEIQEVLDNN
                     PMLEISEDSGPADLPDDINVDFSSEVYSHAEAAPVNSDGDAGSDNSSDAASDNFDSST
                     TTLNEWSDTIPTDLAVDTSWDDIYQSGPTGTGLAKPDNEDMDFESRRGSTETLQDHLY
                     WQLNLTPMSPTDFAIAEAIIDAVDPSGMLCQSIDDIYEGLLGEFPELERDEVIAVLHR
                     VQQFDPVGVAAQNLSESLIIQLRQLPEGTPGWDTAYDIVDEYLDALAQRDFKLLMRKL
                     KVKEEELQTAIALIQSLNPRPGESIGDDTTEYVVPDVFVTKENNRWQVHLNPEIAPKL
                     RINSDYASLVKRADSSSDNTFLRDNLQEARWFLKSLQSRNETLLKVSTCIVERQQGFL
                     EHGPEAMKPMVLHDVAEMVEMHESTISRVTTQKYMHTPQGIFELKYFFSSHVNTDSGG
                     ECSSTAIRAIIKKLIAAENPRKPLSDSKITELLEAQGIQVARRTIAKYRESLNIAPSN
                     ERKRLV"
     gene            4531..4980
                     /gene="ptsN"
                     /locus_tag="PKZ52_09990"
     CDS             4531..4980
                     /gene="ptsN"
                     /locus_tag="PKZ52_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HNG60147.1"
                     /translation="MHLSKLLTPERCRAKMACSSKKRLLEKLAELIAHNTPGISAEVL
                     FSHLVNRERLGSTGIGEGIAIPHCRFETQGATIGALITLAEPIDFDSVDNRPVDIIFA
                     MLVPTDANEQHLATLASLAEALQSANFVEQLRASADDGGLFAAATAA"
     gene            5001..>5798
                     /gene="rapZ"
                     /locus_tag="PKZ52_09995"
     CDS             5001..>5798
                     /gene="rapZ"
                     /locus_tag="PKZ52_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNG60148.1"
                     /translation="MRIVVISGRSGSGKTTALQLLEDVGFTCIDNLPVSLLPALVNQV
                     ASNKDYKFAIGIDARNIDGDLGRFQKIIANSQNLGTPCTVVFLDAANQILLKRFSETR
                     RKHPLTSKTRSLQEAIIAEQAILQPIAEVADFTLDTSNLTLHELRSTIKKMVGGETEG
                     TALLFESFGFKYGLPTYADFVFDVRCLPNPFWNPELRLQTGLEEGVVNFLKGQPEVAA
                     MLEDIDSYLRKWVPSFEANNRSYLTIAIGCTGGMHRSVYLCERLAERF"
BASE COUNT         1374 a         1822 c         1525 g         1077 t
ORIGIN      
        1 atcgaccgag gatcttgcat gttccgcccc ctgggaatct cttgcctggc gctcgccatg
       61 ctcgcctgca gccaacacag cacccaaacc acccccactg ccaacccagc acctgtccaa
      121 ccggacaaac gcgccctctg gccacaacct aaatcccctt ttagcgccgc cgaaacagca
      181 gcagttaatg cccgcgtggc ccagttgctg gcgggtatga gcctggagga aaaagtcggc
      241 caaatggtgg tggccgaaat gggttttatc acccccgagc aagtcaaaga atttagcgtc
      301 ggcgccgtgt tagaagcagg cggcgcctac ctcaacaaaa acaaacaggc gaagatttcc
      361 gactgggtgc tgcaaaccca gcgttattac gtcgcttcgg tggacaaaag cggcggccgc
      421 gcaggcattc caatcctctg gggcaccgat gccttgcacg gccacaacaa agccttcggc
      481 gccaccattt tcccccacaa tattggcctt ggtgctagcc gcaatggcga cctggtgcgc
      541 cgcatcgcag aagtaaccgc cgttgaaacc ctcatcgctg ggaaaaactg gacctatgcg
      601 ccggtggtat cggtggtgcg cgatgaccgc tggggccgca gttacgaaag ctactcggaa
      661 aactccgccc tagtggccga cctgggccgc caggcagtgt tgggcctcca gggtgaagtg
      721 ggcacaacct ttatgcagcc caaccgcctg atggccaccg ccaaacactt tatcggcgat
      781 ggcggcactg aagatggcgt ggaccgcgcc aacaacctcg ccagtgaaga ggatttaatc
      841 cgcatccaca gcccaggtta tgtctccacc ttggaagcgg gcgtgctgtc ggtgatggcc
      901 agccacaact cctggcaggg cacccgcctg cacggccaca agtatttact caccaccatc
      961 ttaaaagaac acatgggctt tgacggtttt gtggtggggg actggaacag ccacggcctg
     1021 attcccggct gcaccaacag cgactgcccg gactccatca atgccggcgt ggatatgatc
     1081 atggtgccgg agcactggaa ggagtttatt cacaccacca tcggccatgt gaaaagcggc
     1141 cgcatcagcc aggcccgcgt ggacgacgcc gtaagccgta ttttgcgcag taaaatccgc
     1201 gcaggcctga tggaatccgg cgagccgatc aaacgcccct acagcaacca aaccgcccaa
     1261 tttaccaacc ccgaacacca cgccctggcc cgccaggcgg tgcgcgaatc cctggtgctg
     1321 ctgaagaaca acggccagct actgcccctc aaccccaagc aaaatatcct gattaccggt
     1381 gaaggcgccg ataacattcc ccaggcttgc ggcggttgga ccatttcctg gcagggtacc
     1441 ggcctggata acaacgactt ccccaacgcc accagcatcg cccagggttt aaagcagcag
     1501 atccaagcgg caggcggcaa ggccatcatc agcaaagacg gcagctttaa aggcaaacct
     1561 gcggtggccg tagtggtgtt cggcgagaag ccctacgccg aatggcaggg ggatgtgaaa
     1621 accctggcct accagccccg cgtgaacagc gatgccaaac tcttagaatc cctaaaggcc
     1681 cagggcatta aaaccgtggc cgtgttttta tccggccgcc ccatgtgggt gaaccgggag
     1741 ctaaacgcca gcgacgcctt cgtggccgcc tggttgccgg gcactgaagg cggcggtatc
     1801 gccgatgtat tgctgcgcaa aaaagacggc accatcgccc acgactttaa aggcaagttg
     1861 tccttctcct ggcctgccaa acccgatcaa accagcaacg aaggcgacgg cagcaccccg
     1921 ctcttcccct atggctttgg cctttcctac gccgccccat tggcggacct aggcccccta
     1981 agcaccgatg tgggcggcgc ggcggattta atcaatggca atacacccat gtttaacggc
     2041 cgcgccaagg ccccttgggt gctcaatatt caatccgcca gcgaagtgca gccctaccaa
     2101 tccagcaccc tcgcaggcaa ggtcatcacc ctcaccgagg cggaccagca cacccaaggg
     2161 gatgcggtgg acgcccagtg gaacggcaca ggcgcaggcc ttttagccct gcaaaccccc
     2221 aaagccagcc aaaacaccac ggatttattg gcccgcaaag gggtagtggc gtttgacctg
     2281 atggtgctgc aaaaacccac cgccaaggtg gagtttgaga tgagctacgc gccgaaatcc
     2341 atcgcccatc gggaggactt caccgctcag ttgggcagcg ccgcgcccaa tacctggcgc
     2401 cgctaccggg tggatatggc ctgttttgcc aaacagaaaa tcgattttag tgagcttaac
     2461 cagggctttg gcctggtcac cagcggcaag ctgcaggtgc gcttagcgaa tgtggtaatg
     2521 gagccggacg aagccgagca agcggatatt aagtgccagt aaacaagcac ttaaattagc
     2581 gctgtacccc ggtgagcatt ttggcaagcc ggggtatttt tttgcctgtg aattagcggc
     2641 atttggccat tatcgttcgg tgaagaggaa tgtccgcagg gcatcccttg tgagtgccca
     2701 aaaggcttta gaggtactga tcagtccaga caataacagg gcaatcaggg tagttgcgcc
     2761 tgggtcaccc ctagggtaaa tgcctcactg ccctgagcag atttttatcc cctgaagcct
     2821 tgacttttct aaattctcaa ttggcctaaa gtttgctttt ttggacgttt aaaagcgttg
     2881 cttgtgccgt agctcctgac tagaattgaa aacagtgccc gaccaacccc gattggttta
     2941 gcccctgttt ttagcctcgc gagctaccgt tctatgaagc aatctctaca gcttaaactc
     3001 ggccagtcac tcaccatgac cccccaactg caacaggcca tccgcctcct gcagctctcc
     3061 accctcgatt tacaggccga gattcaagaa gtactcgata acaaccccat gttggaaatc
     3121 tccgaagact caggcccagc cgatctgccg gacgacatca atgtggattt ttccagcgaa
     3181 gtctacagcc acgccgaagc cgccccggta aacagcgatg gcgacgcagg ttctgacaac
     3241 agcagcgacg cggcctccga caacttcgat agctccacca ccaccctaaa cgagtggagc
     3301 gacaccatcc ctacggatct cgccgtggat accagttggg acgacatcta ccagtctggc
     3361 cccaccggca ccggccttgc caagcctgac aacgaagaca tggattttga atcccgccgg
     3421 ggctccaccg aaaccctgca ggatcacctc tattggcagc tcaacctcac ccccatgtcc
     3481 cccaccgact tcgccatagc cgaagccatc atcgacgcgg tggatccctc cggcatgctc
     3541 tgccagagca tcgacgacat ctacgagggc ctgttaggtg aattcccaga gctggagcgc
     3601 gatgaagtca tcgccgtgtt gcaccgggta caacaatttg acccggtggg cgtggcggcg
     3661 cagaacctca gtgaatccct gattatccag ctgcgccagc tgcccgaagg cacccccggc
     3721 tgggataccg cctacgacat agtggatgaa tacctggacg ccctcgccca gcgggacttc
     3781 aaactgctga tgcgcaaact gaaagtcaaa gaagaagaac tgcaaaccgc catcgccctg
     3841 atccaaagcc tcaacccccg cccgggtgaa agcatcggcg atgacaccac cgagtacgtg
     3901 gtgccggatg tgttcgtcac caaagaaaac aaccgctggc aggtgcacct aaacccggaa
     3961 atcgccccta agctgcgcat caattccgac tacgcaagcc tggtgaaacg ggccgacagc
     4021 agcagcgata acaccttcct gcgggacaat ctgcaagagg cgcgctggtt cttaaagagc
     4081 ctgcaaagcc gcaatgaaac cctgttgaaa gtctccacct gtatcgtcga gcgccaacag
     4141 ggctttttag aacacggccc ggaagccatg aaacccatgg tgttacacga tgtggcggaa
     4201 atggtggaga tgcacgaatc cactatctcg cgggtgacca cgcaaaaata catgcacacc
     4261 ccccagggca tttttgagct gaagtatttc ttctccagcc acgtcaacac cgacagcggc
     4321 ggtgagtgct catcaacggc catccgcgcg attatcaaaa agctcatcgc ggcggaaaac
     4381 ccgcgcaagc ccttaagcga cagcaagatt acggaattat tggaggccca aggtattcaa
     4441 gtggcccggc ggaccatcgc aaaataccgc gagtccctca atatcgcgcc ctcgaacgag
     4501 cgcaaacgtt tagtctgagg taaatgatgt atgcacctga gtaaactgct gacgccagaa
     4561 cgctgccgcg ccaagatggc ctgctccagt aaaaaacgct tactggagaa actggccgag
     4621 ctgatcgcgc acaatacgcc tggcatttca gcagaggttt tattcagtca cctggtcaac
     4681 cgggagcgcc tgggctccac cgggatcggc gagggcatcg ccatccccca ctgccgcttt
     4741 gaaacccagg gcgccaccat cggcgccctc attaccctgg cggaacccat cgacttcgac
     4801 tcagtcgata accgtccggt ggatattatc tttgccatgc tggtgcccac cgacgccaac
     4861 gaacaacacc tagcgacgct tgcaagcctg gccgaagccc tgcagtcggc caattttgtg
     4921 gagcagctgc gcgcaagcgc agacgatggc ggcctgttcg cggccgccac cgccgcttaa
     4981 gggctgggga gaataacaat atgcgcatcg tagtgatcag tggccgctcc ggctcaggca
     5041 aaaccaccgc cctccaattg ctggaggacg tgggcttcac ctgcattgat aacctaccgg
     5101 tgagcctgct gcccgccctg gtcaaccagg tggcctccaa caaagattac aaatttgcca
     5161 tcggcataga tgcccgcaat atcgacggcg atttgggccg ttttcaaaaa atcatcgcca
     5221 acagccaaaa cctcggcacc ccctgcaccg tggtgttttt agatgcggcc aaccaaatcc
     5281 tgctaaagcg cttcagcgaa acccgccgca aacaccccct caccagcaaa acccgcagcc
     5341 tgcaggaggc catcatcgcc gagcaggcca ttttgcagcc cattgccgaa gtggccgatt
     5401 tcaccctgga taccagcaac ctcaccctgc acgagctgcg cagcaccatc aaaaaaatgg
     5461 tcggcggcga aaccgaaggc accgcattgt tgtttgaatc cttcggcttc aaatacggcc
     5521 tgcccaccta cgctgatttt gtcttcgacg tgcgctgcct gcccaatccg ttttggaacc
     5581 ctgagctgcg cctgcaaacc ggcctggaag agggggtggt gaattttctc aagggccagc
     5641 cggaagtggc cgccatgctg gaggatatcg acagctacct gcgcaaatgg gtaccgagtt
     5701 ttgaggccaa taaccgcagt tatttaacta tcgccattgg ctgtaccggc ggcatgcacc
     5761 gctcggtgta tttatgtgaa cgtctggccg agcgcttt
//
LOCUS       DAOQGS010000240         5796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6962_length_5796_cov_5.221738, whole genome shotgun sequence.
ACCESSION   DAOQGS010000240 DAOQGS010000000
VERSION     DAOQGS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6962_length_5796_cov_5.221738"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKZ52_10000"
     CDS             complement(<1..210)
                     /locus_tag="PKZ52_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60149.1"
                     /translation="MSITTKTQQILTLILLAGALTACGGDPSKGASSGGTSSSSSSTS
                     SGGHIKPSSSSSSSSSGKPTSSSSSS"
     gene            506..1858
                     /locus_tag="PKZ52_10005"
     CDS             506..1858
                     /locus_tag="PKZ52_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60150.1"
                     /translation="MHLGNYRWTICGLLFFATTINYLDRQVLSLLAPQLSKEFAWSNT
                     DYANITAVFQFAYAFAMLGAGRLVDKLGTKRGFILAIIIWSLGAMLHAYSVPIGEWGN
                     NLLALAGLAAIPASVLGFMFARMMLAIGEAGNFPAAIKATAEYFPKKERSLATGIFNS
                     GANVGAILAPLSVPWIAEHWGWQTAFIVIGAIGFIWLLFWLHLYDSPAQQKRLSPQEA
                     AYINHNPEDANSAGANEPAMSWFSLLSYRQTWAFALGKFITDGVWWFYLFWLPKFLNG
                     QYGLSGTQLMLPLTVLYSMTMLGSIGGGWFPAYFINKGYAAYDGRLRAMLIIALFPLV
                     VLLAQPLGHFGLWVPIILIGIGASAHQAWSANIFTTVSDMFPRSSVASVVGIGGLAGG
                     MGGVVVSKFGGWLFDWYSAHNAIHTGYAIMFAFCALAYVIAWGVMKTLVPSYRPVAAH
                     "
     gene            2042..2692
                     /locus_tag="PKZ52_10010"
     CDS             2042..2692
                     /locus_tag="PKZ52_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60151.1"
                     /translation="MVKILVVDDHDMVRMGLMRMLADVDGFEVIGEAKNGEEALSMAR
                     ALRPNVILMDVKMPGIGGLEATRRIIAGDPEVKIIAVTAISDDIFPERFMSVGASGYV
                     SKGAHFDEMVKAIKSVCAGRPYMSASVAQQLALRTCSRSKTGSPFEGLSERELQTAIM
                     VANGAKVQSIADSLSISTKTVNSYRYRIFEKLGVTSDVELTILALKHKVLDIDDVN"
     gene            2708..4528
                     /gene="uvrC"
                     /locus_tag="PKZ52_10015"
     CDS             2708..4528
                     /gene="uvrC"
                     /locus_tag="PKZ52_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNG60152.1"
                     /translation="MPEAFDYATFLSHLTTQAGVYQMYDAAGLILYVGKAKNLKNRVS
                     SYFQKTGHTVKTQVLVRRIASITITVTPSEAEALVLEQNLIKAQRPPFNVMLRDDKSY
                     PYIFLSQGEPYPRLAFHRGAKKQPGDYFGPFPSGLAVRDSLLFIARTFGVRQCEDSVF
                     KNRTRPCLQYQIGRCSGPCVGLISPDDYAQDVHHTQQFLRGDDRVLQAELVADMEAAA
                     ERLAFEEAAAIRDKITALRQVQSSSSVEGGQGNVDVVAIASSAGECCIHVLYVRQGRI
                     LGSKSYYPKDQLDEGESHLLEQFLAQQYLSQRGMDLPDAVVLSHPVEGEAGLIEAVRA
                     LRGKTLSIHLRVRAMRAKWLALAQEAAAQNLKMRAASSASALRKLQALQDAVGLAEPP
                     NRMECFDISHSAGEATRASCVVFLEGRAHKTDYRRFSIDGITPGDDYAAMAQALGRRY
                     SRQQAEGKPLPDLLIVDGGKGQLTQAIEVLAQLGLDVPMLGIAKGTTRKAGFETLIFP
                     DGREQTLKDDHPGLHLLQQIRDEAHRFAITGHKNARDKTRRQSRLEDIPGIGPKRRRE
                     LLAYFGGLQEVLGANPEDLARVPGFSKKLAQEVYTALHCE"
     gene            4574..5125
                     /gene="pgsA"
                     /locus_tag="PKZ52_10020"
     CDS             4574..5125
                     /gene="pgsA"
                     /locus_tag="PKZ52_10020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNG60153.1"
                     /translation="MYNLANQLTLMRIALIPMLVVAFYLPFSWKYPLCCGLFILAGIT
                     DWADGFIARRYQLTTPFGAFLDPVADKLMVAVALALLIQAHNSAAFTLPPAVIICREI
                     GISALREWMAEAGNRADVAVSMLGKVKTSFQMTAIAILLAFDPNTNLPMLYIGYAILY
                     TAAILTLWSMVGYLRAAWPSFRK"
     gene            5290..5363
                     /locus_tag="PKZ52_10025"
     tRNA            5290..5363
                     /locus_tag="PKZ52_10025"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5322..5324,aa:Cys,seq:gca)
     gene            5449..5535
                     /locus_tag="PKZ52_10030"
     tRNA            5449..5535
                     /locus_tag="PKZ52_10030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5483..5485,aa:Leu,seq:taa)
BASE COUNT         1250 a         1745 c         1522 g         1279 t
ORIGIN      
        1 ggaagaactg ctggaggacg ttggtttacc ggaagaggaa ctactggatg aactgctggg
       61 tttgatgtgc ccgccggaac tggtgctact ggagctgcta gaagtaccgc cgctgctggc
      121 gcctttagac ggatcgcccc cgcaggcggt gagcgccccg gccagtagta tgagggtgag
      181 aatttgctgc gttttagttg ttatcgacat agtaaagctc ctcgcactag tcacatttta
      241 ttgtgtgtgc atggtagatt gcctgcccac aaaaaactgt gtgaaagtgt aaaacctgcc
      301 ttaacagcta attgaaacaa gtaggcggcg acgctctggc aagagcagta tttcaacaag
      361 cggttaatcg tcttacgaat attcgactgc gtctgaatgt gtaaatagtg tggcaatgcc
      421 cgcagctttc tatggcacgc ccattccaca atatcgcagg ctgaatcaaa gtttcttacc
      481 ccattgccaa aacgagaata attaaatgca cctaggcaac taccgctgga ccatttgtgg
      541 cttattattt tttgccacca ccataaatta tctcgaccgg caagtactca gtttgcttgc
      601 gccacaactc tccaaagaat tcgcctggag taacacggat tacgccaaca tcaccgccgt
      661 gtttcaattt gcctatgcct ttgccatgtt aggtgcgggt cgcctggtgg ataaattagg
      721 caccaaacgg ggttttattc tcgccattat tatttggtcc ctcggcgcta tgctgcacgc
      781 ctattcagtg cccatcggcg aatggggcaa taatctatta gcccttgcgg gcctggcggc
      841 aattccagca tcagtattag gctttatgtt tgcacgcatg atgctcgcca ttggcgaggc
      901 gggtaatttc cccgctgcca ttaaagccac ggccgaatat ttcccgaaaa aagagcgctc
      961 cctcgccact ggaattttta attccggcgc gaatgtgggc gcaattttag cgcccttaag
     1021 cgtgccctgg attgccgaac actggggctg gcaaacggca tttattgtga ttggcgctat
     1081 cggatttatt tggttgttat tttggctgca tttatacgac agccccgccc agcaaaaacg
     1141 cttaagcccc caagaggcgg cttacattaa ccacaatccc gaggatgcta acagcgctgg
     1201 cgcaaacgag cctgccatga gttggtttag cctgttaagt taccggcaaa cctgggcgtt
     1261 tgcgctcggc aaatttatta ccgatggcgt ttggtggttt tatttattct ggctgccgaa
     1321 atttttaaac ggtcaatacg ggctttccgg cacacaatta atgttgccgc tgacggtgct
     1381 gtatagcatg accatgctcg gcagtatcgg cggcggctgg tttccggctt attttattaa
     1441 taagggttac gccgcctacg atggccgcct gcgggccatg ctgattattg cgttattccc
     1501 cctagtggtt ttactcgccc aacccttggg gcattttggc ctgtgggtgc cgattattct
     1561 tatcggtata ggcgcctcag cgcaccaggc ctggtcggcg aatattttta ccaccgtgtc
     1621 ggatatgttc ccccgcagta gcgtggcctc ggtggtgggc atcggcggtt tagcaggtgg
     1681 tatgggcggg gtggtggtgt ccaaattcgg cggctggttg ttcgactggt acagcgcaca
     1741 caatgccatc cacaccggtt acgccattat gttcgccttc tgcgccctgg cctatgtcat
     1801 cgcctggggt gttatgaaaa cgctggtgcc cagctatagg cctgtggccg cacattaatg
     1861 catgcacctg tgccaattca ccgcgtgcca ggttagtctg tagctacttg cggttaagag
     1921 cgcacagcat ctgccgatat aggcgctacc tctaggcaaa ttccccgcca caatggtaaa
     1981 ttcgccgccg cttgcctgag ggaagttgcg ccttgtccag aagcttagag acttatcgcc
     2041 catggtaaaa attttagtag tcgatgatca cgatatggtg cgcatggggc tgatgcgcat
     2101 gctggcggac gtggacggtt ttgaggtgat cggcgaggcg aaaaacggcg aagaagccct
     2161 cagcatggcc cgcgccctgc gccccaatgt aattttaatg gacgttaaaa tgcccggcat
     2221 cggcggcctg gaggccacgc ggcgcatcat cgccggggac ccagaggtta aaattatcgc
     2281 cgtcaccgct atttccgatg atattttccc ggagcggttt atgagcgtgg gcgccagcgg
     2341 ttatgtcagc aagggcgctc attttgatga aatggtcaaa gccattaaat ccgtctgcgc
     2401 aggccgcccg tacatgagcg catcggtggc ccagcagctg gcgctgcgta cctgcagccg
     2461 cagcaaaacc ggctccccct ttgagggcct gtccgagcgg gagctgcaaa ccgccatcat
     2521 ggtggccaat ggcgccaagg tgcagtcgat tgccgattcc ctctccatca gcaccaaaac
     2581 cgtcaacagt tatcgctacc gcatttttga aaaactcggg gttaccagcg atgttgaact
     2641 cactattctc gcgttaaaac acaaagtcct cgatatagac gatgtgaatt aggccctttg
     2701 tttccccgtg ccagaagctt ttgattacgc cactttttta agccacctga ccacccaagc
     2761 gggggtctac cagatgtacg acgcggcggg gctaatcctt tacgtcggca aggccaaaaa
     2821 ccttaaaaac cgcgtcagca gttatttcca aaaaaccggc cacacggtga aaacccaggt
     2881 gctggtccgg cgtatcgcct ctatcaccat caccgtcacc cccagtgaag ccgaagccct
     2941 agtgctggag caaaacctca tcaaagccca gcggccacct tttaatgtga tgctgcggga
     3001 tgataaatcc tacccctata ttttcttatc ccaaggagag ccctacccgc gcttggcatt
     3061 tcatcgcggc gccaaaaaac agcccggcga ttatttcggc cccttcccca gtggcttagc
     3121 cgtgcgcgat agcctgctgt ttatcgcccg caccttcggc gtgcgccagt gcgaggattc
     3181 cgtatttaaa aaccgcaccc ggccctgttt gcagtatcaa atcggccgct gcagcggccc
     3241 ctgcgtgggg ctgatcagcc ctgacgacta cgcccaggac gtacaccaca cccagcaatt
     3301 tttgcgcggc gacgaccggg tattacaggc ggaattggta gcggatatgg aagccgctgc
     3361 cgagcgccta gcctttgaag aggccgctgc cattcgcgat aaaatcaccg ccctgcgcca
     3421 ggtgcaatcc tccagctccg tggagggcgg ccagggcaat gtggatgtgg tggccatcgc
     3481 cagctccgcg ggggaatgct gcatccacgt actctacgtg cgccaaggcc gcatcctcgg
     3541 cagcaaaagt tattacccca aagaccaact ggatgaaggc gagagccact tgctggagca
     3601 atttctcgcc cagcagtacc tctcccagcg gggcatggac ctgccggatg ccgtggtgct
     3661 cagccacccg gtggagggcg aggcgggcct catcgaagcg gtgcgcgccc tgcgcggcaa
     3721 aaccctcagc atccacctgc gggtgcgcgc catgcgcgcc aaatggttag ccctggccca
     3781 agaggccgcc gcacaaaact taaaaatgcg cgccgccagc tccgcctccg ccctgcgcaa
     3841 attgcaggcc ttgcaagatg cagtgggcct tgccgaaccg cccaaccgca tggagtgttt
     3901 tgacatcagc cacagcgcag gcgaagccac ccgcgcctcc tgcgtggtgt tcctggaagg
     3961 ccgcgcccac aaaactgact accgccgctt cagcatcgac ggcatcacac cgggcgatga
     4021 ctacgccgcc atggcccaag ccctcgggcg ccgctacagc cgccaacagg ccgaaggcaa
     4081 acccctgccg gacctgctca tagtggatgg cggcaagggc cagctcaccc aagccattga
     4141 ggtgctcgcc cagttggggc ttgatgtgcc catgctcggc attgccaagg gcaccacccg
     4201 caaagcaggc tttgaaaccc tgatcttccc ggatggccgc gagcaaaccc tcaaagacga
     4261 tcaccccggc ctgcatctct tacagcaaat ccgcgacgag gcccaccgct tcgccatcac
     4321 cggccacaaa aacgctcgcg acaaaacccg ccgccaatcc cgcctggagg acatccccgg
     4381 cataggcccc aaacggcgcc gcgagttatt ggcttatttt ggcggcctac aggaagtgct
     4441 cggcgccaat cccgaggatt tagcccgcgt gccgggcttc agcaaaaaat tggcccaaga
     4501 ggtttacact gctttgcact gtgagtaacc tgccctaagc ggccccgcct agctccatct
     4561 gttcgagaat tccatgtata acctcgccaa ccagctcacc ctgatgcgca tcgcattaat
     4621 ccccatgctg gtggtggcct tttacctgcc cttcagctgg aaataccccc tctgctgcgg
     4681 cctgtttatc ctcgctggca tcaccgattg ggccgacggt tttatcgccc gccgctacca
     4741 actcaccaca ccctttggtg cctttttaga cccggtggcg gataaactca tggtggccgt
     4801 cgccctcgct ctgctcattc aagcccacaa cagcgccgcc ttcaccctgc cccccgccgt
     4861 gattatctgc cgggaaatcg gcatctccgc cctgcgcgaa tggatggccg aagcgggcaa
     4921 ccgcgccgat gtggccgtct ccatgctggg caaggtgaaa accagttttc aaatgacggc
     4981 catcgccata ctgcttgcct tcgatcccaa cactaatctg cctatgctct acatcggcta
     5041 cgccatcctt tataccgccg ccattcttac cctttggtcc atggtgggct acctgcgcgc
     5101 cgcctggccg agctttcgca aataacaacc gcgcctgtta atcgtcctct ctaaccaacc
     5161 aggaccacgc tctaagcact cccccaaacg catttttgca ggctggaata gagccaggcc
     5221 cgcgtttaag aatttttccg ccagggctgt tttttgccct caacttgagt agaatgcgcc
     5281 ccctgttaag gcgcgatagc aaagcggtta tgctccggat tgcaactccg tttagcccgg
     5341 ttcgactccg ggtcgcgcct ccaaataaat gctcttcctg agcacagatg taaaagcgct
     5401 ctcacctttg agcgcatcca taccgacgag cccagctcga tcctccccgc ccgggtggtg
     5461 aaatcggtag acacagggga cttaaaatcc cccggccgcg aggccgtgcc ggttcaagtc
     5521 cggccccggg caccatttcc ttattcagca tcatctaaaa cagtcctaaa gcccccgcaa
     5581 atactggctt taagcctatt ctcgctccaa ccacgtccag ccgaatccat tgaaagccag
     5641 aagctctggg ggcataattg ggggcacagg cgcgcagggt tggggcacag atggcaggca
     5701 aagataagct cactgaaaag gcagtcaagc tggcgaagcc gaaggaaaaa tcctaccgct
     5761 tagccgacgg cggcggcatg tacttagagg ttatgc
//
LOCUS       DAOQGS010000241         5778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_6995_length_5778_cov_5.227503, whole genome shotgun sequence.
ACCESSION   DAOQGS010000241 DAOQGS010000000
VERSION     DAOQGS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6995_length_5778_cov_5.227503"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..527
                     /gene="rplQ"
                     /locus_tag="PKZ52_10035"
     CDS             132..527
                     /gene="rplQ"
                     /locus_tag="PKZ52_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNG60154.1"
                     /translation="MRHRLSGRQLNRTSSHRKAMFSNMVASLVEHELIKTTLPKAKEL
                     RTYAEPLITLSKVDSVANRRLAFNRLRSKAAVGKLFNELGPRYNARPGGYLRIIKCGY
                     RTGDKAPMAYVELVDRPIVGDLESAASAE"
     gene            complement(630..842)
                     /locus_tag="PKZ52_10040"
     CDS             complement(630..842)
                     /locus_tag="PKZ52_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60155.1"
                     /translation="MGGTIEVDEPLLVADSDRRNILSALANGQAAEKLLSENQLGITA
                     RRGEVTNELRLSYKRISWQTKNNTIN"
     gene            1097..2197
                     /locus_tag="PKZ52_10045"
     CDS             1097..2197
                     /locus_tag="PKZ52_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNG60156.1"
                     /translation="MAEYEVEKAMRSWLLLLLCLMMLPCAQASEVRMEDFMALPAPLQ
                     LALDTRIKTLPRRTEQVAALHQLLYSPAGFNITYNNQRTKTAAEAFYTRSGNCLSLAA
                     LYVASARYLGMDARFQAVEIPFQWLQRDNTMYYLGHVNALVKLGHNRVTAEFLGAFTA
                     EESAKFKSVEMSDAEALARYYNNLGVEAMVRQEPSKAFDYLQAAIEKNKKYADAWVNL
                     GVWYKSQQRGLDAEQAYLTAVHLESKNTSALSNLMVLYQAQGRTQMAAQVAAKLHKHR
                     LRNPYYLANLAERALQEKDYATAVSLLKKAVKKQEEGHFYQLLGQAYFGLGEKSKSQI
                     ALEKSVALAANPQDKSLRSGKLAALRSMSAGH"
     gene            complement(2239..3273)
                     /locus_tag="PKZ52_10050"
     CDS             complement(2239..3273)
                     /locus_tag="PKZ52_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate dehydrogenase family protein"
                     /protein_id="HNG60157.1"
                     /translation="MYGVSEMMCILCWYLGSNHKKRAATQTQLLEGVMKVSIIGLGHV
                     GSAIAFVLSLKSIIKELVLVGRTPESVMGDVLDLRHGQLFVDAPSKISAGNLNDTADS
                     DVLVVCASVPMDANMQDRQVLGPANVKLMASLLPQLAHYSPQAKLVMVTNPVDILVYF
                     AIEFTGFAPQRVLGTGTLVDSARFRQLLAEDVQIHTEDIRAYILGEHGQTQFPAMSVA
                     TAGGEAIDDTPQRRELFKRASQAGFEVLRRKGHTNYAIALAAVDIIHNIITDTKHTMP
                     VSLKSDGFLGVSDVCLSLPAVIGKNGIERILHPKLNTEEQAAFLHSANHLRAIITAIK
                     DELKTPAAGA"
     gene            complement(3435..4352)
                     /locus_tag="PKZ52_10055"
     CDS             complement(3435..4352)
                     /locus_tag="PKZ52_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNG60158.1"
                     /translation="MPIPLVANPVYSYPFPAQHRFPMEKFALLQNYVAAQGWLVNNPF
                     YRPGKASLNLLQLAHCPAYLQRFIHNQQSPAELKAMGLPWSEALVRRTLISPNGTLLT
                     ALLALEKGIACHLAGGTHHAHANFAAGFCILNDLAVAALALIKQRKVKRVLIFDCDVH
                     QGDGTARILENHPTIFTCSIHCEANFPSEKARSHLDIALPRGTQTQDYLLTLQTTLMQ
                     AIALSQPDIILYDAGVDIYAQDPLGHFAVSEAGIYERDLFVLNTCKSLGIPVATVIGG
                     GYDDNRAALAARHALVIKAAVEVFTAQSR"
     gene            complement(4502..4627)
                     /locus_tag="PKZ52_10060"
     CDS             complement(4502..4627)
                     /locus_tag="PKZ52_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60159.1"
                     /translation="MSISRTLLTALHGRQIEGNLAKRRVDRGAAVVNTCIHHCEG"
     gene            5007..>5778
                     /locus_tag="PKZ52_10065"
     CDS             5007..>5778
                     /locus_tag="PKZ52_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNG60160.1"
                     /translation="MSKAEMDRLGWDSCDIIIISGDAYVDHPSFGAAIIGRLLESQGF
                     RVGIIAQPDWSSAEAFKELGKPNLFFGVSAGNMDSMINRYTSDLRLRSDDAYTPNNEP
                     GKRPERAVTVYAQRCKEAYKNTPVVLGGIEASLRRIAQYDYWSDKVRRSVLDDATADI
                     LLYGNAERAIVDLAHALAAGKTLADISYLRGTVVIRDAIPSGWSEIDSSRIDWPKHID
                     SIPNPYEMKEQNTQSCKTENGGEANPAMEEPVRIIPMPL"
BASE COUNT         1384 a         1349 c         1538 g         1507 t
ORIGIN      
        1 gattgtcttt gggtatgcgt ttagagaact ggccgcccgc cagtttgaaa aatagcgact
       61 aaatgttaac aaaaggccct gtgggcctta gatatgactg ctgagaaagc aatcaacaaa
      121 gggtttaagt tatgcgtcat cgtttaagtg gtcgccagct aaatagaaca agctctcacc
      181 gcaaggctat gtttagcaac atggttgcgt cattggttga gcatgagttg atcaaaacaa
      241 ctctgcccaa agccaaagag ttgcgtacct atgctgagcc tttaattact cttagcaaag
      301 tggatagcgt tgccaatcgc cgtttggcgt ttaaccgtct gcgcagtaaa gccgccgtcg
      361 gtaaactctt caacgaactg ggccctcgct acaacgctcg cccaggtggt tatctgcgta
      421 ttatcaagtg cggctaccgc accggtgata aagcgcctat ggcctatgtg gagttagtgg
      481 atcgcccgat tgtgggtgat ttggaatctg ctgctagcgc tgagtaagtt cttcgcttcg
      541 taatcggagc taaaaagcca gccttagggc tggctttttt tgtttgatcg cctgtatcta
      601 aagtaaaagc cagcctattc cgcagtgttt tagttgatgg tgttgttttt tgtctgccag
      661 ctaattcgtt tgtaggaaag ccttagctcg tttgttactt ctcccctcct tgccgttatg
      721 cccaactgat tttcagacag taatttttct gctgcttggc cattggcaag cgcgcttaaa
      781 atatttcttc tatcgctgtc ggcaacgaga agtggctcgt cgacttctat cgttcctccc
      841 atttgatatt ccttcaggca ttagtgggtg ctgtttttat acgcatgccc tagagaccag
      901 cccaatgtat gctttaggta agcagatcat aagcccagcg cagctgtaac catattaagc
      961 tttagatagc gaggaaggtt tatgggtgcg ggaagggtgt ggcatttgct cccgatcaga
     1021 tagaggggga ttaaaagcgc ctgctcgacc taataaattt ctggccccag ttgtacaaaa
     1081 ccgaaaggtt tttattttgg cagaatacga ggtggaaaaa gctatgcgca gttggctgtt
     1141 gttattgctg tgcctaatga tgttgccttg tgctcaggcg agcgaggtgc gcatggagga
     1201 ttttatggct ctgcctgcgc ctttgcagct tgcactggat acccgtatta agacattgcc
     1261 gcgccgcacg gaacaagttg ctgcgctgca tcagctgctg tatagcccgg ctggctttaa
     1321 tatcacttat aacaatcagc gcaccaaaac cgccgctgag gccttttata cgcgctcggg
     1381 taattgttta tcgctagctg cattgtatgt agcgtcggcg cggtatttgg gtatggatgc
     1441 gcggtttcaa gcggtagaga ttcccttcca gtggctgcag cgggataaca ccatgtatta
     1501 cctgggccat gtaaacgcat tggtgaagct cggtcataac cgcgtgacgg cggaatttct
     1561 cggcgccttt acggctgagg agtcagccaa atttaagtcg gtggaaatga gtgatgccga
     1621 ggcgctggcc cgttattaca ataatttggg tgtggaggct atggtgcgcc aggagcccag
     1681 caaagcgttt gactatttac aggcggctat cgagaaaaat aaaaaatatg ccgacgcctg
     1741 ggttaatttg ggggtttggt ataaaagtca gcagcgcggg ttggatgctg agcaggccta
     1801 cttaacggct gtgcatcttg agagtaaaaa tacttcagcc ctgagcaatt taatggtgtt
     1861 gtatcaagcc caggggcgca cccaaatggc agctcaggtt gcggctaagc tgcataagca
     1921 taggttgcga aatccctatt atttggcgaa tttggctgag cgcgcgctgc aggaaaagga
     1981 ttatgctacg gcggtgagtc ttttgaaaaa agcggtaaaa aaacaagaag aggggcattt
     2041 ttatcaatta ttggggcagg cgtattttgg tttgggtgaa aaatccaagt cgcaaatagc
     2101 gctggaaaaa tcagtggcac tggcggcaaa cccacaggat aaatccttgc gctcaggaaa
     2161 attagcggcc ctacgcagta tgtcggcggg gcactaaggc gtgtttttta agccgtgaaa
     2221 atcactctgg cgcggttgtt aagctccagc cgcaggagtt tttagctcgt cttttatagc
     2281 ggtaataatt gcccgcaggt gattggcgga gtgtaaaaaa gcagcttgtt cttcagtgtt
     2341 gagcttgggg tgcaatatac gctcgatgcc atttttgcca ataaccgcag gaaggcttag
     2401 gcatacatca ctaacaccta aaaagccgtc ggattttaaa cttaccggca tggtgtgttt
     2461 ggtgtcggta ataatattgt gaataatatc caccgcagcg agcgcaatgg cgtaattggt
     2521 gtggcccttg cgccgcaata cttcaaaacc cgcttgggag gcgcgtttaa ataattcccg
     2581 gcgctggggt gtatcgtcga ttgcctcgcc accggctgtg gctacactca tggccgggaa
     2641 ttgggtttgg ccatgttcac ccaaaatata ggcgcgaata tcttcggtat gaatctgtac
     2701 atcctccgcc aataattgcc gaaagcgggc tgagtccacc agtgtgccgg tgcccaatac
     2761 ccgctggggg gcaaagccgg taaattcgat ggcgaaatac accagtatat cgaccggatt
     2821 agtcaccatg accagttttg cctgtggcga atagtgcgct aattgcggta ataaactggc
     2881 cattaatttt acattggcgg ggccgagcac ttggcgatct tgcatattgg catccatggg
     2941 cacagatgca cacaccacta atacatcgct gtcggcggta tcgtttaaat tgcctgcgga
     3001 aattttgcta ggggcatcta caaataattg cccgtggcgc aaatccaaaa catcccccat
     3061 taccgactca ggtgtgcgcc ctaccaaaac taattctttg attatgcttt ttagcgacag
     3121 tacaaacgca atggctgatc ctacgtggcc taggccgata atggagactt tcataacacc
     3181 ctctagcaat tgagtttgtg tggcggcgcg ttttttatgg ttactaccta ggtaccagca
     3241 aagaatacac atcatttcag acacgccgta catacatccg tgcaggctcg tcaaaaaggt
     3301 ctctcttttt gacggtctga aatgatgtgt attctttgct tccgtacagc tattaagtgt
     3361 gtgaatggtg agaatttaaa taattatgga acactgctgt agttaaagtt taattcgagc
     3421 atgctgatga ataattaacg gctttgcgcg gtaaacacct ctaccgccgc ttttatcacc
     3481 agtgcgtggc gggcggcaag cgccgctcga ttatcatcat agccgccacc tatgaccgtt
     3541 gccactggaa tgccaaggct tttacaggta tttaatacga ataaatctcg ctcataaata
     3601 cccgcttcgc tgaccgcaaa atgccctaag ggatcctgag cgtaaatatc cacccccgca
     3661 tcgtacagaa taatatccgg ttggctgagg gctattgcct gcatcagagt tgtttgcagg
     3721 gttaataaat agtcttgggt ttgggtgccg cggggtaggg ctatgtctaa atggctgcgg
     3781 gctttttcgc tggggaaatt agcttcgcag tgaatggagc aggtaaaaat cgtcgggtga
     3841 ttttctaaaa tgcgcgccgt gccatcgccc tggtgtacgt cacaatcaaa aatcagcacc
     3901 cgttttacct tgcgctgttt aatcagcgcc agagcggcca cagctaagtc gttgagaata
     3961 caaaaacctg cagcgaaatt agcgtgggcg tggtgggtgc cgccggccaa atggcaggca
     4021 atgccttttt ccagcgccaa cagtgcggtg agcagtgtgc cgttggggga aattagtgtg
     4081 cgccgcacca gggcctcact ccagggtagg cccatggctt taagttcggc aggcgattgt
     4141 tgattgtgaa taaaccgctg caaataggcg gggcagtggg ctaattgcaa cagatttaaa
     4201 ctcgccttgc cggggcggta aaacggattg tttacaagcc agccttgagc cgctacataa
     4261 ttttgtaaca gcgcgaattt ttccatcggg aagcgatgct gggcggggaa gggataggaa
     4321 taaaccggat tggctacaag ggggatcggc ataattaatg tcgcctggga aattcacaca
     4381 aaaatagtta agcgttgtga gttaaaactc taattgattg tttatcctca gccccttttt
     4441 agggtggctg gatctgatgc ttgcgcctaa tagaaatcat cgcgtgagcg ctttagtgca
     4501 gctagccctc gcagtggtga atacaggtat tcaccactgc tgcaccgcga tcgactctac
     4561 gcttggctaa attcccttct atttgccgcc cgtgcagagc ggttaatagg gtgcgcgaaa
     4621 tagacataca ctttaattga ttatttatct atgggttcgg tgttgttact aatcgagatg
     4681 tgctctcagc gccctcgcaa tgagtgtatg cggcggtgct aggttttttt aactatcatt
     4741 tggggttgga tcaccgcccg ctggccgcac tcaatcccca ttaatgacgc aaaaaccgct
     4801 tattttatag ggccaaaaga gcgaaatagg cactgccggg cgattgtgat ttctggcaca
     4861 atggccgcct atttgcctgc cttgtcgtgc cccccatgtc tgcccctgct gctaaacctg
     4921 cccaatttgt gcccttaaac gcaccgaata ttttttcgtt ccctaaatat tgggcggcct
     4981 gttttgaacc tgcgcctttt ttgccgatga gcaaagcgga gatggaccgc ttaggctggg
     5041 atagctgcga catcataatt atcagtggcg atgcctatgt agaccatccc agttttgggg
     5101 ctgccattat cggccgcctg ttggagtccc aaggctttag ggtcgggatt atcgcccagc
     5161 cggattggag cagtgctgaa gcctttaagg aactgggtaa accgaattta ttttttggcg
     5221 tcagcgcggg caatatggat tccatgatca accgctacac atctgatctg cgcctgcgca
     5281 gcgacgatgc ctatacccct aataacgaac cgggtaagcg cccggagcgg gcggtgacgg
     5341 tatacgccca gcgctgtaaa gaagcctata aaaatacgcc tgtggtgctc ggcggtatcg
     5401 aggcgagttt gcggcgcatc gcccagtacg actactggag cgataaggtg cgccgcagtg
     5461 tgttggatga tgccaccgcc gatattctgc tgtacggcaa tgccgagcgg gccattgtgg
     5521 atctggccca cgccctggcg gcgggtaaaa ctctggcgga tattagttac ctgcgcggca
     5581 cggtggtgat tcgcgatgcc attcccagcg gttggagcga aattgattcc tcccgcatcg
     5641 attggcctaa gcacatcgat agcatcccca acccctatga aatgaaggag cagaacaccc
     5701 agagctgcaa aaccgaaaac ggcggcgagg caaaccccgc catggaagag cctgtgcgca
     5761 ttattcccat gccattgc
//
LOCUS       DAOQGS010000242         5749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7040_length_5749_cov_4.259220, whole genome shotgun sequence.
ACCESSION   DAOQGS010000242 DAOQGS010000000
VERSION     DAOQGS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7040_length_5749_cov_4.259220"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PKZ52_10070"
     CDS             <1..936
                     /locus_tag="PKZ52_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG60161.1"
                     /translation="LGCADILVRNGVKPVVFDRYPEIGGLLTFGIPEFKLEKSVMRRR
                     REVFQEMGVEFRLNTEIGKDISIDELLRDYDAVFMGMGTYTYMKGGFPGEDLPGVYDA
                     LPFLVSNVNRNLGFEKDPADFISVKGKRVVILGGGDTAMDCNRTSIRQGATSVTCAYR
                     RDEENMPGSRREVQNAREEGVTFLFNRQPVAIVGDGKVEGVKVVTTALGAPDERGRRK
                     PEIIPGSEEILPADIVLVAFGFKPNPAPWFAEHGIVTNAWGGVEAPEAQTYKFQTKNP
                     KVFAGGDMVRGSDLVVTAIWEGREAAEGILDYLQV"
     gene            1287..2357
                     /gene="hemE"
                     /locus_tag="PKZ52_10075"
     CDS             1287..2357
                     /gene="hemE"
                     /locus_tag="PKZ52_10075"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNG60162.1"
                     /translation="MSSLQNDRFLRALLRQPVDRTPVWMMRQAGRYLPEYRATRAKAG
                     NFMGLCMNPELACEVTLQPLERYALDAAILFSDILTIPDAMGLGLYFGEGEGPKFKKA
                     LTSAADIEALPVVNTERDLGYVLDAVRLIRRELNSRVPLIGFSGSPWTLATYMLEGGS
                     SKDFRRSKTLMYNQPEVMHALLDTLVASVIDYLNAQIRAGAQAVQIFDTWGGALSAAA
                     YQEFSLKPMQRIVDGLLKEHEGRPVPVILFTKGGGLWLESMANTGASALGLDWTCDIG
                     GARQRVGHKVALQGNMDPGILYASPERIRAEVSAILKAYGRGSGHVFNLGHGITPEVD
                     PAHAGAFISAVNELSAEYHESH"
     gene            2563..3954
                     /locus_tag="PKZ52_10080"
     CDS             2563..3954
                     /locus_tag="PKZ52_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNG60163.1"
                     /translation="MGIKQIKIDVNELTLGMFVSGLDRPWTQTPFPLQGFYINTLEEI
                     QLLKVHCNHAFIDIAKGPGPVKTNLRTLSNPGGAASAPKSANKIKVTDVAPIKVQHNQ
                     YPVAESLGREIVRARELHQQVYAAIGEVMRQLGTGNELPIQETKRTASAMVDSVLRSP
                     DAFTWLSRVKEKDEYTYSHAVRCAVWGILFGRHIGLPKVDLDVLALGLLLKDLGKTRL
                     PLELLEKQNRSAAEQAAYEKFVDFGVEILRELPDIKPRVTSVVKTHCERLNGTGFPEQ
                     LRGDKIPLLGKIAGIVTFYDETTTPRGENRSYSPSKAVGRLYEVRDELFQSDLVVEFI
                     RAIGLYPTGTLVELNTGEVAVVVEQNFERRLKPKIMIVLDTLKKPLSEPVFLDLALDE
                     KAKQAKLDAGKVTAAEVERIEIAQDLDPSAYDLDINEIRDVYLRRQTSPNKGFLSFLK
                     GNRALAGIFGGRD"
     gene            complement(4093..5112)
                     /gene="galE"
                     /locus_tag="PKZ52_10085"
     CDS             complement(4093..5112)
                     /gene="galE"
                     /locus_tag="PKZ52_10085"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNG60164.1"
                     /translation="MKVLVTGGAGYIGSHTCIELIQAGHQVVVVDNLANAKREALTRV
                     EAIVGTAIPFYLADVTDRAALDTVFRQESPIDAVIHFAGLKAVGESCEKPGDYYRVNV
                     YGSLVLADVMERHGVPLLAFSSSATVYGDPHTVPIREDFPTGATNPYGRSKLMVEEIL
                     QDLAKRTNNTLKIALLRYFNPIGAHESGTIGEDPKGIPNNLLPYVAQVAVGTLPCLRV
                     FGNDYPTPDGTGVRDYIHVVDLARGHLAALHRLSDSAPGAYIWNLGTGRGFSVLEVVD
                     AFAKASGREIPYAIVPRRPGDIAECYADPSLAAAELNWRAHYDLTRMVTDAWRWQHRN
                     PNGFA"
     gene            5470..>5749
                     /locus_tag="PKZ52_10090"
     CDS             5470..>5749
                     /locus_tag="PKZ52_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNG60165.1"
                     /translation="MTIFSHYRERYASTQKEELSIQEYLELCKSDPSVYASAPERMLL
                     AIGEPELVDTARDPRLSRIFSNKIIKRYKAFEDFYGMDEAIEQIVSFFK"
BASE COUNT         1319 a         1484 c         1596 g         1350 t
ORIGIN      
        1 cttggttgtg ccgatatatt ggtgcgcaac ggcgtgaaac ccgtggtgtt tgaccgctac
       61 ccggagatcg gcggcctgct gacctttggt atcccggaat ttaaattaga aaaatccgtt
      121 atgcgccgcc gccgggaagt gttccaggag atgggggttg agtttcgcct caacaccgag
      181 atcggcaaag acatcagcat agatgagctg ctgcgcgact acgacgcggt gtttatgggt
      241 atgggtacct atacctacat gaaaggcggc ttccccggcg aggatctacc cggcgtttac
      301 gatgccctgc cgtttttggt ttccaacgtc aaccgcaacc tggggtttga aaaagacccg
      361 gctgatttca tcagtgtgaa gggtaagcgt gtggtgattc taggtggtgg cgacaccgct
      421 atggactgta accgcacctc catccgccag ggcgccacta gtgttacctg tgcctaccgc
      481 cgcgatgaag aaaacatgcc cggctcccgc cgcgaagtac aaaacgcgcg ggaggaaggc
      541 gtgacctttt tatttaaccg ccagccggtg gccattgtgg gcgacggcaa ggtggaaggc
      601 gtaaaagtag ttaccacagc attgggggcg cctgatgagc gcggtcgccg caagcccgag
      661 attatccccg gcagtgaaga aattttgccc gctgacatag tattggtagc cttcggcttt
      721 aaacccaacc cagccccctg gtttgccgag cacggcatag tcaccaatgc atggggcggt
      781 gtagaggcgc ccgaggcgca aacctacaag ttccaaacca agaaccccaa ggtgtttgcc
      841 ggcggtgata tggtgcgcgg ctccgaccta gtggtaactg ccatttggga aggccgggaa
      901 gccgccgagg gaatcttgga ctatcttcag gtgtaacacc tgctctcggc gcggcctgca
      961 aacccaggcc gcgccaaaat atgaaactgc gccctaggtt cctgacactc caatcattcc
     1021 gtgttagtgt gcagttgcaa attggcaagg gggcgcagtt atcagtcatt gggccccagc
     1081 caggctgaaa tcagaataat aaatactgga taaaaaggtt ggacaaccga gcttttgggg
     1141 ccttgctaag cagggtatct gcagggttac gggcaaggtc caaaccataa taaatagttg
     1201 aatcgacggc cttgcaggct gaatcgatgc tttatcccca ggcgctgccc ggtactgcca
     1261 ggcgctgcta gttactgaga gtcactatga gttcattgca aaacgaccgc tttttgcgcg
     1321 ccctgctgcg ccaacctgtg gatcgcacac cggtttggat gatgcgccag gctggtcgct
     1381 acctgcctga gtaccgggcc acccgcgcca aggcgggcaa ttttatgggc ctgtgtatga
     1441 accccgaact cgcctgcgaa gtaacgctgc agcccctaga gcgctacgcc ctggatgcag
     1501 ccattttgtt ttccgacatt ctcaccatcc ccgacgccat ggggctcggt ttgtatttcg
     1561 gtgagggcga ggggcctaaa tttaaaaaag ccttaactag cgccgccgat atcgaagcct
     1621 tgccggtggt gaatactgag cgggacctgg gctatgtgtt ggacgccgtg cggcttattc
     1681 gccgtgagtt aaatagtcga gtgcccttaa tcggcttctc cggcagcccc tggaccctgg
     1741 ccacctatat gttagagggc ggctccagca aagattttcg ccgctcgaaa accctgatgt
     1801 acaaccagcc agaagttatg catgcgctgc tggatacatt ggtggcatcg gttattgact
     1861 acctcaacgc acaaattcgc gcgggcgctc aagcagtaca gatttttgat acctggggag
     1921 gggcgctatc ggccgccgcc taccaagaat tctcccttaa gcccatgcaa cgaattgtcg
     1981 atggtttgct gaaagaacac gagggccgcc cggtgccggt gattttgttc accaagggtg
     2041 gcggcctgtg gctggagtcc atggcgaata ccggcgcctc cgccttaggc ctggattgga
     2101 cctgcgatat aggcggggcc cgtcagcgcg tgggccataa ggtcgccctg cagggcaata
     2161 tggaccctgg aattttatat gcctcccctg agcgtattcg tgcggaagtg tctgccatac
     2221 tcaaggctta tgggcgcggc agcggccatg tgtttaacct cggccacggt attacaccgg
     2281 aggtggaccc cgctcatgcg ggtgcgttta tctcggctgt gaatgagttg tcagccgagt
     2341 atcatgagtc gcattaggcg cgggatctat ttagcgcaga atgggtcgca gcggagggtg
     2401 aatttgccgc tccttctgaa caccccgacc catttgcaaa aataatttgg gacagttccc
     2461 aaatatcgaa tccttttgtg ttggttgtat tttattcaaa ttggctggtt tataatcacg
     2521 tttgcaatca atttaaacat catctagcga gagggggtta acttgggtat taagcaaatt
     2581 aaaatcgatg ttaatgaact caccctcggc atgtttgtat ctggcttgga taggccttgg
     2641 acgcagaccc cttttccttt acagggcttt tacatcaaca cgctagaaga aatccagctg
     2701 ttgaaagtgc attgcaacca cgcctttatt gatatagcca agggcccagg gccagttaaa
     2761 accaacctgc gcaccctgag caaccccggc ggcgctgcca gtgcgcctaa atctgccaac
     2821 aaaataaaag tgaccgatgt agcgccgatc aaggtgcagc ataaccagta tcctgtggcg
     2881 gaaagccttg gccgggagat agtccgcgcc cgtgagttgc atcaacaggt gtatgccgcc
     2941 atcggcgaag ttatgcgtca gttgggcacg ggcaatgaat tgcccattca agaaaccaaa
     3001 cgcactgcca gcgccatggt agatagtgtg ttgcgcagcc cagatgcctt cacttggctg
     3061 agccgggtga aagaaaaaga cgagtacacc tacagccacg cggtgcgctg cgccgtgtgg
     3121 ggtattttat tcggccgcca cataggcttg cctaaagtgg atttggacgt gctcgcgctc
     3181 gggttactgc ttaaagacct gggtaaaacc cgcctgcctt tggaattatt ggaaaaacaa
     3241 aaccgcagcg ccgccgagca ggccgcctat gagaagtttg tggatttcgg tgtggagatt
     3301 ctgcgcgagc tgcccgatat taaaccgcgt gtaacctcag tggttaaaac ccactgtgag
     3361 cgccttaatg gcacgggttt tcccgagcaa ttgcgcggcg acaaaattcc cctgctgggc
     3421 aaaatcgccg ggatcgtcac cttctatgat gagaccacca caccgcgcgg tgaaaatcgc
     3481 agctattcgc cctccaaagc cgtgggccgc ctgtatgagg tgcgcgatga gttgttccaa
     3541 tcggacctgg tggtggaatt tatccgcgcc attggcctgt accccacggg taccttggtg
     3601 gagttaaata ccggggaagt ggcggtggtt gtcgagcaaa atttcgagcg gcggttaaag
     3661 ccaaaaatta tgattgtgct ggataccctg aaaaaaccgc tctccgaacc ggttttttta
     3721 gacctggctc tggatgaaaa ggctaaacaa gcgaaactgg atgcgggcaa ggttaccgcc
     3781 gctgaggttg agcgtatcga gatcgcccag gatttagacc ccagcgccta tgatctggac
     3841 atcaatgaaa tccgcgatgt ttacctgcgt cggcaaactt cccccaacaa gggcttttta
     3901 tcctttttga agggcaatcg cgctttagca ggtatttttg gcggccgaga ctagaattgg
     3961 cggcctgaat tgggcgggtt tgagatccgc tgaagctaag caagctgaag ctaagcaaat
     4021 tgcgacttga tctttttgtc agcggcccgg caggttgtac ctgccgggcc gtttcttttt
     4081 tcaccgataa ccttaagcaa agccattggg attgcgatgt tgccagcgcc aggcgtctgt
     4141 caccatgcgg gtaagatcat agtgcgcgcg ccaatttaat tccgccgccg cgaggcttgg
     4201 atcggcataa cactcggcta tatcgcccgg gcggcgaggt acgattgcgt agggaatttc
     4261 cctccccgat gccttagcaa aggcatccac cacctccaac acactaaatc cgcgcccagt
     4321 gcccaggttc caaatatagg cgccgggggc actgtcgctc aggcggtgta gggccgccag
     4381 gtggccgcgg gcgagatcca ccacatgaat ataatcgcgc acacccgtac catcgggagt
     4441 tgggtagtcg ttgccaaaaa cacgcaggca gggcagggtt cctacggcaa cctgtgccac
     4501 ataaggcagc aaattattgg ggattccctt tgggtcctca ccaatcgtgc cactttcatg
     4561 tgcgccgatg ggattaaaat agcgcagaag ggcaattttc aaagtgttgt ttgtgcgttt
     4621 agcgaggtcc tggagaatct cttccaccat taacttgctg cggccatagg gattggtggc
     4681 accagtagga aaatcttcgc ggatgggtac tgtgtgggga tcgccataaa cagtggccga
     4741 agaactaaag gccagcagtg gcaccccgtg gcgctccatc acatcggcca ggactaaaga
     4801 gccgtataca ttaacgcgat agtagtcgcc gggcttttcg caggactcgc ctaccgcttt
     4861 taagcctgca aagtgaataa cggcgtcaat gggggattcc tgtctaaata cagtgtccag
     4921 tgcggcgcga tcagtgacat cggccagata aaacggtatt gcagtgccga caatggcctc
     4981 gacgcgggtg agtgcttcac gcttggcgtt ggccaaatta tcgaccacta caacctgatg
     5041 gcctgcctgt atcagctcaa tacacgtatg ggagccgatg tagcctgcgc cgcctgttac
     5101 taataccttc atggggatgc tcgttatttg cgtgtgtgat gtatgaatta tttcacggat
     5161 gtatcaatat aacaaaacca tagcatttga cgtcggcaat gggcgcgccc ttaataaaac
     5221 cagtgcgaat ttaccctagc cgtggcgtag attttgaatg gctcgattct gtagtcagta
     5281 tttgctcaat tgtgaccggt tgattgacat aatcttggca aaagtgccgg aattaggttg
     5341 ggcagcattt ttttgacggt tccaagcatc tttttgggga attttgtcgg ctgaactatt
     5401 tccaacgtct atgcttggaa tagtcgccgc acaatccctc gcaatttcgt cataactgag
     5461 gagctctcta tgaccatttt tagtcattac cgtgaacgtt atgcttcaac acaaaaagaa
     5521 gaattgagta ttcaggaata ccttgagctc tgtaaatcag acccttccgt ttacgcctcg
     5581 gcccctgagc gtatgctact tgccatcggc gagcctgagt tggtggacac cgcgcgggat
     5641 ccgcgtctta gccgtatttt ttccaacaaa atcattaagc gctacaaggc gtttgaggat
     5701 ttttacggta tggatgaggc cattgaacag atagtgtcgt ttttcaaac
//
LOCUS       DAOQGS010000243         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7046_length_5745_cov_3.916696, whole genome shotgun sequence.
ACCESSION   DAOQGS010000243 DAOQGS010000000
VERSION     DAOQGS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7046_length_5745_cov_3.916696"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2433)
                     /locus_tag="PKZ52_10095"
     CDS             complement(<1..2433)
                     /locus_tag="PKZ52_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNG60166.1"
                     /translation="MLNPALIEPPDAAARRRALTPDQSFIVEAPAGSGKTELLTQRIL
                     TLLAQVPTPEAIVAITFTRKAAAEMHARLVQALKAGQGSEPANPHQAFTWQLARQVLA
                     QDAKHGWQLLKNPGRLRISTFDSLCASLTQALPLQSKLGHQLSISEDPQELYAQAIED
                     LLASLAEATAPLSGPLASLASLLAHLDNRAERLAHLLTEVLARRDAWRPLLVELRSLP
                     DERRRAILEAHLAERCDEHYAWLAEQFAPIWPELRKIAYEAALRLEGNPKGAAIARLN
                     TIDQYQMPPSEGEEAQVIWLGLQNLLLTRSGTFRINLDVNCGFPAGTSKAEKEQAKAD
                     KAAMKELIFTLAGCPGLLDALIAVEHLPSRQYSDEQWQVLAAVLDVLPRLLAHLRLVF
                     GRSGQLDFTEVALSAEQALGDEEAPTDLALRLDYQIQHLLVDEFQDTSPSQVELLRRL
                     TLGWQPGDGRSLFCVGDAMQSIYSFRAADVSLFLHIKAHGLGDLYLEPLQLTANFRSA
                     PSLVHWVNRVFAKAFPEISDIASGAVAYSPAEPFRPEQPAQVHYLSLAPGLGEAAQAE
                     LLVEQVLAHRADAPEDTIAILVRNRAHGQYFLRLAQSAGLTPLAVDMQALAEHPAVQD
                     AWVLTQALFNPADTIAWLSLLRGPWAGLSLSDLLQLRQQPGVTVMEQIPLCLAADVLP
                     ADAQLRLGRAWAVLENAWRERGRKPARWWLEGTWLALGGASCLGQEEDISNLERYWLC
                     LESLAQQGQALSLTRIATALGKLYAAPSPKADARLQIMTIHKSKGLEFDAVFLPSLHK
                     GKRPADKQLWLWQ"
     gene            complement(2426..4672)
                     /locus_tag="PKZ52_10100"
     CDS             complement(2426..4672)
                     /locus_tag="PKZ52_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNG60167.1"
                     /translation="MELERRGALCSAQVAPILAQLLTDEPGSAHIRPLPEALWLTGFD
                     DINPAQEALLKALNGRGCGLHYFEPQRQVNACLLPCNDEAEELASAAAWAKERLQAEP
                     QARIGWVIPDLPSRKLAVERVLTQVFSPQDFHIQEARHAPGYNISVAEPLGFTPPIAA
                     ALHLLNLVAAPADSEDWQLFATSPFIGSEGEVPWRARLLGQITRRYHQITWRRLLSAT
                     DAFKGQDAAPSWQQSLTQTLAWRRALPRQRLCIGQWLEHWQVLLAIWHWPGQRQLDTL
                     EYQQLMQWPVLLADVQQLEAVLGPVSFTEALGHLSRLSYRPFAAQTGDSPLQVLGLLE
                     AAGQPFDELWVMGMDGRHWPPPAQPNPLLPIALQRSKLMPRSSHQRELMLAERLTQRL
                     CQAAPRVLVSFSLREGDRPLVPSPLVEHLPLYSGPDVALPSSVAEQLRPGSVPLEAFR
                     DDQGPCLAAQQEGGTQVLKDQAACPFAAFARHRLNAQSVQPLLFGVNPGLRGSLLHSV
                     LFQLWQAWQSQDVLMDLQAGAVEQAIEAALAQSWLELDPFMGVAQRVREIENRRCQRV
                     VNALLEHERQRPPFSVATLEAKQQIQLGQVRLNLRLDRVDRLADGGQVVIDYKSREAS
                     HQLWLDERPADPQVPLYCLLTPEVVGGLFAVLEPGKEGFTGLMEAGRDLLGVPAPEQL
                     KNTEISSWPELLTLWQQRLQALADEFSQGIATTTPSPNVCRFCDLASLCRYKERAAGL
                     VAEEVEDA"
     gene            complement(4606..5109)
                     /locus_tag="PKZ52_10105"
     CDS             complement(4606..5109)
                     /locus_tag="PKZ52_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60168.1"
                     /translation="MRTDAVYDPLFAAISPGAWVLTPNRRLARVLINAYNRYQQSIGR
                     LAWQAPNILAWADGLELLWQRAFASLGPKQCPQLGYRFLSDDEALTLWQNLLSSQAQG
                     WDLLKPESLAGPAFSAWQSLQLWPVWMRAWRKPLYLRPQLGNLNWNWSGEGPCAVPRW
                     HPYWLSS"
     gene            5274..>5745
                     /locus_tag="PKZ52_10110"
     CDS             5274..>5745
                     /locus_tag="PKZ52_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNG60169.1"
                     /translation="MPQATQPSNQPLALIVMGVSGCGKSTLAAHLANTYGFEYIEADD
                     YHTEEAKRLMASGTPLTDAHREPWIQALCDKLKQLAASGKSSVLAYSGLRAAHRARFR
                     ALGIPVQFIHLVGDKELIRSRMQARKNHFMPSGLIDSQFAALESTEAETDVIAIS"
BASE COUNT         1165 a         1819 c         1711 g         1050 t
ORIGIN      
        1 ctgccacagc cagagctgtt tatccgccgg gcgcttgccc ttgtgcaggc tgggcaggaa
       61 caccgcgtca aactccaggc ccttggattt atggatagtc ataatctgta ggcgcgcatc
      121 tgccttaggg cttggagcgg cgtagagttt gcccagagct gttgcaatgc gggtcaaact
      181 cagcgcctgc ccctgctggg caaggctctc aaggcaaagc cagtagcgct caagattact
      241 tatgtcctct tcttggccga ggcaactggc gccgcccagg gcaagccagg tgccctccag
      301 ccaccagcgg gccggtttgc ggccgcgctc ccgccaggcg ttctcaagta ccgcccaagc
      361 gcggcctaag cgcagctggg catctgcagg taaaacgtcg gcggcaagac acagggggat
      421 ctgctccata accgttaccc ccggctgttg ccgcagctgc agcagatcac tcaggcttaa
      481 gcccgcccag ggcccccgca gcaggctcag ccaggcgata gtatccgccg gattaaacag
      541 cgcttgagtt aacacccagg cgtcctgcac cgccgggtgc tccgccaggg cctgcatgtc
      601 caccgccagg ggcgtaaggc ctgcactttg agccaggcgc agaaaatact gaccgtgggc
      661 gcggttgcgc accaggatgg caatggtgtc ctcaggtgcg tctgcgcgat gagccagcac
      721 ctgctccacc aataactctg cctgggccgc ttccccaagg ccaggggcga ggcttaggta
      781 gtgcacctgg gcgggttgct ccgggcggaa gggttcggcg gggctatagg ccaccgcccc
      841 gctggctata tcgctgatct cggggaaggc tttggcgaac actcggttca cccagtgcac
      901 aaggcttggc gcactgcgaa agttagccgt cagttgtaga ggctccaggt ataggtcccc
      961 caggccatgg gccttgatgt gcagaaatag gctcacatcc gctgcgcgaa aactgtaaat
     1021 ggactgcatg gcatccccca cgcaaaacag gctgcggcca tcccccggct gccaaccgag
     1081 ggttaagcgc cgcagcagct ccacctggct gggggaggtg tcttggaact cgtccaccaa
     1141 caggtgttgg atttgataat ccagccgcag ggccaagtcc gtgggcgcct cttcatcccc
     1201 taaagcctgc tcggcgctta gggctacctc ggtaaaatcc agctggccgc tgcggccaaa
     1261 taccaggcgc aggtgggcaa gcaggcgggg caatacatcc agcacggccg ctaaaacctg
     1321 ccactgctca tcgctgtact ggcggctggg caggtgctcc accgctatga gggcgtccaa
     1381 caaaccgggg cagcccgcca gggtaaaaat cagctccttc atggcggctt tatcggcctt
     1441 ggcctgttct ttttccgcct tgctggtacc cgccggaaag ccgcagttca catccaggtt
     1501 gatgcgaaag gtgccggagc gggttagcag caggttttgt aaccccagcc aaatcacctg
     1561 ggcctcttcg ccttcactag gcggcatctg gtattgatca attgtgttta agcgggcaat
     1621 ggcagccccc ttggggttgc cctctaaacg cagagcggcc tcataggcaa ttttgcgaag
     1681 ttccggccag atgggtgcaa actgctcggc aagccaggcg tagtgttcat cgcagcgctc
     1741 ggctagatgg gcttccagaa tcgcccggcg cctttcatcg ggcaggctgc gcagctccac
     1801 cagcaggggc cgccaggcgt cccggcgggc cagtacctca gtgagcaggt gggccaaccg
     1861 ctccgcccgg ttatccaaat gggccaagag gctagcaagg cttgccaggg ggccgctcag
     1921 gggcgcagtt gcttcggcga ggcttgccaa caggtcttca atggcctggg cgtataactc
     1981 ctgcggatcc tcactgatgc tcagctgatg gccgagtttg gactgcaggg gcagagcctg
     2041 ggtgagggag gcgcagaggc tatcgaaggt ggagatacgc aggcggccgg ggtttttcag
     2101 cagttgccag ccgtgtttgg catcctgggc caacacctgc cgggccagct gccaggtaaa
     2161 ggcctggtga ggattggcag gctcactgcc ctgccccgcc ttgagggcct gcaccaggcg
     2221 ggcgtgcatc tctgccgcgg ctttgcgggt aaaggtgatg gccacaatgg cctcgggcgt
     2281 aggcacctgg gccaatagag taagaatgcg ctgggtgagc agctcggttt tgccgctgcc
     2341 cgctggggcc tccacgataa aggattgatc gggggtgagt gcccggcggc gggcggcggc
     2401 atcgggcggt tcgatcaaag cagggttaag catcttccac ctcctcggca accaagcctg
     2461 cggcccgttc cttgtagcgg cagaggctcg ccaaatcaca gaagcgacac acgtttgggc
     2521 tcggggtggt ggtggcaatt ccctggctga actcatcggc cagggcctgc aagcgctgct
     2581 gccagagggt caaaagctcc ggccaactgc taatttcagt atttttaagc tgctccggcg
     2641 caggcacccc aagcagatcc cgccccgctt ccataaggcc ggtaaagccc tccttacccg
     2701 gctccaacac cgcaaacagc ccgcccacca cctcaggagt gagcaggcaa tagagcggca
     2761 cttgggggtc cgccgggcgc tcatccagcc acagttggtg gctcgcctcg cgggatttgt
     2821 aatcgatcac cacctggccg ccatcggcta agcgatccac ccggtccagc cgcaggttca
     2881 agcgcacctg ccccaactgg atctgctgct tagcttcaag cgtagccaca ctaaacggtg
     2941 gccgctggcg ttcgtgctcc agcagggcat taactacccg ctggcagcgg cggttttcaa
     3001 tctcccgcac ccgctgggca acccccataa atgggtctag ctccaaccag ctttgggcca
     3061 gagccgcctc aatagcctgc tcaaccgccc ctgcctgcaa atccatcagt acatcctggc
     3121 tctgccatgc ctgccagagt tgaaacaaca cggagtgcag caggctgccc cgcaggcctg
     3181 ggtttacccc aaacaacaac ggctgcaccg attgcgcgtt cagccgatgg cgggcaaaag
     3241 cggcaaaggg gcaagcggct tgatctttta acacctgggt gccgccttcc tgctgggcgg
     3301 ccaaacaagg cccctggtca tcccgaaagg cttctaaggg cactgagcct gggcgcaatt
     3361 gctctgccac tgaactaggc aaggcaacgt ctggcccgct gtagaggggt aaatgctcca
     3421 ccaaagggct tggcaccagg gggcggtccc cttcgcgcag gctgaagctc accaataccc
     3481 ggggcgccgc ttggcacaaa cgctgggtta aacgttccgc cagcatcaac tcccgctggt
     3541 ggctggagcg cggcatgagt ttagagcgct gcagggcaat gggtaataag ggattaggtt
     3601 gggccggtgg cggccagtgg cggccatcca ttcccatcac ccacaattca tcaaagggct
     3661 gcccggccgc ttccaacaaa cccaatactt gcaggggcga atcgccggtt tgggcggcaa
     3721 aagggcggta gcttaagcgg cttaaatggc ctaaagcttc agtaaaactc accggcccca
     3781 gcaccgcctc cagctgctgc acatccgcca gcaacaccgg ccactgcatc aactgctgat
     3841 attccaacgt atccagctgc cgctgccccg gccagtgcca aatggccaac aggacctgcc
     3901 aatgctccag ccactggcca atacataatc gctggcgagg tagagcccgc cgccaggcga
     3961 gggtttgggt gaggctctgc tgccagctgg gggcggcatc ctggccctta aacgcatcgg
     4021 tggcggagag cagccgccgc caggtgattt gatgatagcg ccgcgtgatc tgccccaata
     4081 ggcgtgcccg ccaaggtacc tccccttcgg agccgataaa cgggctggtg gcaaacagct
     4141 gccaatcctc gctatcggcg ggggccgcca ctaggttcag cagatgcaaa gcggcagcga
     4201 tgggtggggt gaagccgagg ggttccgcca cggagatgtt ataaccgggg gcgtggcgcg
     4261 cctcctgaat gtgaaaatcc tggggggaaa acacctgggt gagcacccgc tccaccgcaa
     4321 gcttacggct gggaagatcg ggaattaccc agccgatccg ggcctgaggc tctgcctgca
     4381 atcgctcttt ggcccaagcg gcggcagagg ccagctcttc ggcttcatca ttacagggca
     4441 acagacatgc attcacctgg cgctggggtt cgaaataatg aagcccgcag ccccggccgt
     4501 taagtgcctt taacaaagcc tcttgggctg ggttgatatc gtcaaagccg gttaaccata
     4561 gggcttcggg cagtgggcgg atatgtgctg agccaggctc atcggttagg agctgagcca
     4621 atatgggtgc cacctgggca ctgcacaggg cccctcgccg ctccagttcc aattcaaatt
     4681 gcccagctgc ggccttaaat acagcggttt ccgccaggct ctcatccaca ctggccatag
     4741 ctgcaggctc tgccaggcgc taaaggcggg gcccgccagg ctctcaggct ttaataggtc
     4801 ccagccttga gcttggctac ttaataaatt ttgccagagg gtcagggcct catcgtcgct
     4861 taaaaatcgg taaccaagct gggggcactg ctttggcccc aggctcgcaa aggcccgctg
     4921 ccacagcagc tccagcccgt cggcccaggc gagtatgttc ggcgcctgcc aggccaggcg
     4981 ccctatagac tgctgatagc ggttgtaggc gttaataagc acccgcgcca aacggcggtt
     5041 gggggtaagc acccaggcac ccggcgagat tgccgcaaat aatgggtcat acaccgcatc
     5101 ggttcgcacc gcttactccc cctatgcaaa agccgcgacg gtacaaagcc tgcgccacaa
     5161 tagcaagcgg gccgaggaat ccccttgcca acaggcccaa ggccgccgca aacaattggc
     5221 ccctaggcgc ctgcatgtta gccttgcgct tttaacccag tgccggaacc accatgcccc
     5281 aagcaacaca gccatccaat caaccgctcg cccttattgt gatgggcgtc agcggctgcg
     5341 gtaaatctac tcttgccgct catctagcta acacctatgg ctttgaatac atagaagccg
     5401 acgactacca cacagaagaa gccaaacgcc taatggccag cggcaccccc ctcaccgacg
     5461 cccaccggga gccctggatt caagccctct gcgacaaact caagcagcta gccgccagcg
     5521 gcaaaagctc tgtcctcgcc tactccggcc tgcgcgccgc ccaccgcgct cgctttcgcg
     5581 ccctcggcat acccgtgcaa tttattcatt tagtgggcga taaagaactg atccgcagcc
     5641 gcatgcaagc gcgcaaaaat cactttatgc cgtcagggtt aatagacagc caatttgccg
     5701 cgcttgaatc caccgaagcg gaaactgatg tcattgctat ttctg
//
LOCUS       DAOQGS010000244         5688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7140_length_5688_cov_4.970886, whole genome shotgun sequence.
ACCESSION   DAOQGS010000244 DAOQGS010000000
VERSION     DAOQGS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7140_length_5688_cov_4.970886"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(563..2281)
                     /locus_tag="PKZ52_10115"
     CDS             complement(563..2281)
                     /locus_tag="PKZ52_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60170.1"
                     /translation="MLRLIHCHKNKQPMRPVSSLVVSVLFAATLLASLPSFALEADEC
                     KHLDWSVDDLAKFKQSQFPSDFYQPFAGKTIRHVRIETGPVFDRTNPKENNWLYRSLD
                     ALHINTKPQVIASQLLFRAGELLDEDKVLESARILRTRGYLASAYILPEAVCGDAVDL
                     VVMTRDVWSTEPDTSLSRNGGENKSGFGISEGNLLGYGNELAIGYETVAERSAINYDF
                     TSPHIFNSHYYAHLSYADKTDGKDKIVELAKPFYSLQTPYAYGVTSKTITEAEILRYG
                     GVKRSAFLHSQERYEIFAGHALVVSDAATTRLIGGVAMDEDEYAATAATRTPLPADEV
                     NRYPWLAVRKIENHFGAFKNLQQIQRVEDVLLGSDLYVRLGYAAENFRNERDGLLMQA
                     YYSDVLASGDNHLLQFKSSIALKDYAGSAERFALTQHLTYNHFLSDHSRWYVDAQWDY
                     GKRLLPQDEFTLGGASGLRGYPLEFQRGQERYLLTTEYRYFSDWHLFNLLRVGGVVFA
                     ETGKAWNSQIQTGQETLSDVGFGLRISSSKARVGSVVHIDIAMPTASREGIDSYQLVV
                     STLQTF"
     gene            complement(2299..2655)
                     /locus_tag="PKZ52_10120"
     CDS             complement(2299..2655)
                     /locus_tag="PKZ52_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60171.1"
                     /translation="MKLPAPANSADFALLTSPDGEFWLAYELARDFLSAPRHYAVLAG
                     SLGGQGELVCLQPPQGIADEEFIYQEGERFIISLANSRQELKLAARDISLLVTLPHPE
                     GPQAALLASLGRLFTG"
     gene            complement(2652..3194)
                     /locus_tag="PKZ52_10125"
     CDS             complement(2652..3194)
                     /locus_tag="PKZ52_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNG60172.1"
                     /translation="MNNSTGVSFSRPLIILHWAVAALVLALLAAVELHEVFPKDSLMR
                     GLFMKTHFWLGLSVGLLTLVRLWLRWGQHPAPITPAPALWQTRLAAGVQWAFYGILLV
                     MPVLGYLLINAKGFDLSLGPISLGHWVAADKELVETLEELHETLGNVFIALVALHTVA
                     ALYHHHILKDSTFSRLQPPA"
     gene            3307..5619
                     /gene="bglX"
                     /locus_tag="PKZ52_10130"
     CDS             3307..5619
                     /gene="bglX"
                     /locus_tag="PKZ52_10130"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNG60173.1"
                     /translation="MTHTVLRALGLAALLLSKPSLALTDQQIEDLIKQMTPEEKAGQL
                     NIIAGTQNATDVIPGLPSPEKHMAMIKAGQVAGFFNINNSDYIRQLQRIAVEESRLKI
                     PILIAGDVIHGYRTTTPLPLAEAASWDLDLIRRSARMAAEEAAASGVNWTFAPMVDIS
                     RNPRWGRIIEGAGEDPYLGSAIARARVKGFQGDDLSAPDTIAATVKHFAAYGAPEAGR
                     EYNSVDMSERSLREAYLPPYKAALEAGAATVMSAFNTLNGVPAVMDPFLMRQILRNEW
                     QFNGTVVTDYEAIKELIAHGVAKDLQDAALKAFNATTDMDMMANAYTGHLPALLAEQK
                     ITEPQLNDAVRRILQLKNRLGLFEDPYKYLPDAKTRDAIVFSDKHRALARDIAKRSIV
                     LLKNDKKLLPLPKKIRTLAVIGPMADSKEDQNSTWALFSKPDDPVSILQGIKNAVDPK
                     TKIISAAGCSFLFACDAKDIAKAVNLALQADYVIMALGEPTSLVGEDGARTSLDFPGH
                     QSNLLRAIADTNKPVILLVSSGRPLTLMNAEKKADAILQTWHLGTEAGNAVADVLFGD
                     YNPSGKLPMSFPRSIGQVPLFYNHLPTGRPTDNKAQPEANTRTYKSRYVDVANSALYP
                     FGYGLSYTDFTYSNLRLSSNTLSMGSGITASVTLTNTGKMAGEEVAQLYIHDPLASVS
                     RPVKELKGFQKILLQPGESRDISFEITPDMLAFYRLDMTYGLEAGDFEVFIGGNSDTE
                     KSVRFTLLEPPPVAPPAKKTSGKKPPKARS"
BASE COUNT         1477 a         1595 c         1384 g         1232 t
ORIGIN      
        1 tttttgacga gcctacacgg atgtattcac ggcgtgtctg cactaacctg tagtcgccac
       61 tgcgggcgga gtattgtggg ggtcacagca ctaaagcact cgcctgacgg cttccagtcg
      121 gcgaaagcat cacgcccaac caatataaaa gacagctgct caaaaacaaa aaaccagcca
      181 aaaaattact gtttggaata tttgaaatag ccttggcagt aaaacagaag caggtgccgc
      241 tataaagcaa tcacaaccat ttagtatttt tttgggttag catttttatt tgctggtgtt
      301 ttttcatatg aatgagcgaa attcccgagc agacgagtat cacatggtga gcagcgtaac
      361 gcacgcatgc ccacccgcag caatgaatat aggttgtttc agacacgccg tgaatacgtc
      421 catgtaggct cgtcaaaaag atccctcttt ttgacggcct gaaacaacct atattcattg
      481 ctgctgcacc gcgagtcatt gtgcacactg gcttaatttt cgcttgcgcg ggtgaaaaaa
      541 gtgctgttac aaacgatcaa gcctaaaacg tctgcaaggt agacaccacc agctggtaac
      601 tatcaattcc ctcgcgagaa gccgtcggca tagcaatatc aatatgcacc actgaaccta
      661 cacgcgcttt agatgagcta atccgcaggc caaaacccac atccgaaagc gtttcctgac
      721 cggtttgtat ttggctattc cacgccttgc cggtttcggc aaataccacc ccgcccaccc
      781 gcagtaaatt aaacaagtgc cagtcggaaa aatagcggta ttcggtggtt aataaataac
      841 gctcctggcc gcgctgaaat tccagcggat aaccgcgcaa accgcttgca ccgccgaggg
      901 taaattcatc ctggggcaat aaacgcttgc cgtaatccca ctgggcgtcc acataccagc
      961 ggctgtggtc gcttaaaaaa tggttgtagg ttaaatgctg ggttaaggca aaccgctcgg
     1021 cgctgcctgc gtaatctttt agggcaatgg aggatttaaa ttgcaacaga tgattatcgc
     1081 cactggccag cacatcagaa taatacgcct gcattaaaag accatcgcgc tcgttgcgga
     1141 aattctcggc ggcataacct aagcgcacat acaaatcgct gcccaataaa acgtcctcca
     1201 cccgctgaat ttgctgcagg tttttaaacg caccgaaatg attttcaatt ttgcgtacag
     1261 ccagccaagg gtagcgattc acttcatcgg caggcagcgg cgtgcgggta gcggcagttg
     1321 ccgcgtattc gtcttcgtcc atggcaacac cgccaattaa gcgcgtggtg gcagcatccg
     1381 agaccaccaa tgcgtgccca gcaaaaattt cgtaacgctc ctggctgtgc aaaaacgccg
     1441 agcgcttaac cccgccatag cgcagaattt ccgcctcggt gatggtttta ctggttacac
     1501 cataggcata aggcgtttgc agggaataaa acggcttggc caattcaaca attttatcct
     1561 tgccatcggt tttatccgca taggacaaat gggcgtaata atgggaatta aaaatatgcg
     1621 ggctggtgaa gtcgtaatta atggcgctgc gctcagccac ggtttcataa cctatggcaa
     1681 gttcattgcc atagcctaat aaattgccct cgctgatacc aaagccagat ttattttcac
     1741 cgccgttgcg gctcaaactg gtatccggtt cggtggacca cacatcgcgg gtcatcacca
     1801 ctaagtcaac tgcatcgcca cacacagctt ccggcaaaat ataagcgctg gctaaatacc
     1861 cgcgtgtgcg caaaatccgc gcggattcaa gcaccttatc ttcatctaac aattcccctg
     1921 cgcgaaataa taattggctg gcaattacct ggggtttggt attgatgtgc agggcatcca
     1981 aactgcggta cagccaatta ttttctttag ggttggtgcg atcaaatacc ggcccagttt
     2041 caatgcgcac atggcgaatg gttttaccgg caaagggctg ataaaaatcg ctgggaaatt
     2101 ggctctgttt aaatttggct aagtcatcca ccgaccaatc caagtgtttg cactcatcgg
     2161 cctccagggc aaagcttggg agcgatgcta agagcgtggc agcgaataaa acagagacaa
     2221 caagcgaaga gacagggcgc atgggttgtt tatttttatg gcagtgaatt aagcgcagca
     2281 tagcccaaaa gccagccatt aacccgtgaa gagccgcccc aaactggcca aaagtgccgc
     2341 ctgcggcccc tccgggtggg gcaaggtcac cagcaggcta atgtcccgcg ccgccagctt
     2401 cagctcctgg cgactattgg cgaggctgat aataaaacgc tcgccctcct ggtagataaa
     2461 ttcttcatcg gcaataccct gcggaggttg caggcacacc agctcacctt gcccgcctag
     2521 gctgcccgcc aacacggcat agtgccgggg tgccgataaa aaatcccgcg ccagctcata
     2581 ggccaaccag aactccccat ctgggctggt gagcagggca aagtcagcgg aattggcggg
     2641 cgcgggcagc ttcatgccgg tggttgcagg cggctgaagg tgctgtcttt aagaatatgg
     2701 tggtggtaga gggccgctac cgtatgcagc gccaccaggg caataaacac attgccgagg
     2761 gtttcgtgca gctcttctaa agtttctact aattctttat ccgccgccac ccaatggccg
     2821 aggctaatcg gcccgaggga taaatcaaag cctttggcgt taataagcag ataacccaac
     2881 accggcatca ccaacaaaat accgtaaaaa gcccactgca cccccgccgc caaacgggtt
     2941 tgccagaggg ccggcgcagg tgtaatcggc gccgggtgtt ggccccagcg cagccacagg
     3001 cgcaccagtg tgagcaggcc cacacttagg cccaaccaaa aatgcgtttt cataaacaaa
     3061 ccgcgcatca ggctgtcctt ggggaagact tcatgcagct caacggcggc gagcagcgct
     3121 aagactaacg cggccacggc ccaatgcagg ataataagcg ggcggctaaa gctgacaccg
     3181 gtactattgt tcataaactt gtcatccgaa gagaggggtg cttaagtcta attataggga
     3241 ccctgggctt atgcccagct taactagcta ttcactatct tggcgagagc cattacgaga
     3301 ccactcatga cccatactgt tttgcgcgcc ctcggccttg cggcgctgtt gttaagcaag
     3361 ccgagccttg ccttaaccga tcaacaaatt gaagacctga tcaaacaaat gaccccggaa
     3421 gaaaaagccg ggcaattaaa tatcatcgcc ggcacccaaa acgccactga cgtgattccc
     3481 ggtttaccca gcccggaaaa acacatggcg atgattaaag caggccaagt ggccggtttt
     3541 tttaatatta ataattccga ctacatccgc caattacagc gcattgccgt ggaagaatcg
     3601 cgcttaaaaa ttcctatttt gatcgcgggt gatgtcatcc acggctaccg caccaccacc
     3661 cccctgcccc tggcagaagc cgccagctgg gatttggacc tcatccgccg ctctgcccgc
     3721 atggccgctg aagaggccgc cgcctctgga gtgaactgga ccttcgcccc catggtggat
     3781 atttcccgca acccgcgctg gggccgcatt attgaaggcg caggcgaaga tccttaccta
     3841 ggctccgcca tcgcccgcgc ccgggtgaag ggctttcagg gcgatgactt atccgccccg
     3901 gatactatcg ccgccaccgt caaacacttc gccgcctacg gcgcccccga agccgggcgg
     3961 gaatacaact cggtggatat gagcgagcgc agcctgcgcg aagcctattt accgccctat
     4021 aaagccgccc tggaggcggg cgctgccacc gtaatgagcg cgtttaatac cctcaacggt
     4081 gtgcccgccg tgatggaccc ttttttaatg cggcaaattc tgcgcaatga gtggcaattt
     4141 aacggcacgg tggtaaccga ctacgaagcc attaaagaat taatcgccca tggcgtggcc
     4201 aaagatttac aagatgccgc cctaaaagcc tttaacgcca ccaccgatat ggacatgatg
     4261 gccaatgcct acaccggcca tttaccggca ttattagcgg agcaaaaaat taccgagccc
     4321 caattaaacg atgcggtgcg gcgcattttg caattaaaaa atcgcctagg tttatttgaa
     4381 gacccgtata aatatttacc cgatgccaaa acccgcgacg ccatagtgtt ttccgataaa
     4441 caccgcgccc tcgcccgcga catcgccaaa cgctccatag tgttgttaaa aaacgataaa
     4501 aaattattgc cgctgccgaa aaaaatccgc accctggcgg tgattggccc catggccgac
     4561 agcaaagaag accaaaattc cacttgggcg ttattttcca aaccggatga tcccgtctcc
     4621 attttgcaag gcattaaaaa tgcggtggac ccaaaaacca aaattatttc cgccgctggc
     4681 tgcagctttt tatttgcctg cgacgctaaa gacatagcca aggccgtcaa cctcgccctg
     4741 caagccgatt atgtgattat ggccttaggc gaacccacct cattagtggg tgaagacggc
     4801 gcccgcacct ctttagattt ccccggccat caaagcaatt tattgcgcgc cattgccgac
     4861 accaataaac cggttatttt attagtcagc tccggccgcc ctttaacgct tatgaacgcc
     4921 gagaaaaaag ccgacgccat tttacaaacc tggcacctag gcaccgaagc gggtaatgcg
     4981 gtggctgatg tgttatttgg cgattacaac cccagcggca aattgcccat gagtttcccg
     5041 cgcagtatcg gccaggtgcc actgttttat aaccacttac ccaccgggcg gcccaccgat
     5101 aacaaagccc agccggaagc caatacccgc acctacaaaa gccgctacgt ggacgtggcc
     5161 aactccgccc tttacccctt tggctatggc ctaagctaca cggatttcac ctacagcaat
     5221 ctgcggttaa gcagtaatac gctgagcatg ggcagcggca ttaccgccag cgtcaccctt
     5281 actaacaccg gcaaaatggc aggtgaagaa gtggcccagc tgtatattca cgaccctctc
     5341 gccagcgtgt cccgcccggt aaaagaatta aaaggctttc aaaaaattct gctgcagccg
     5401 ggtgaaagtc gcgatattag ttttgaaatc accccggata tgctggcttt ttaccgtttg
     5461 gatatgacct atggccttga agcgggggat tttgaagtgt ttatcggcgg caattcggac
     5521 acagaaaaat ccgtgcgctt cactctttta gagccgccgc cggtggctcc gccagcgaaa
     5581 aaaaccagcg ggaaaaaacc gccaaaagcc cgctcataag ctgcgcctaa ctctgcgtgc
     5641 aactcgtctg cgccagggtg cgcaggcggg tttgctttta ccagatcc
//
LOCUS       DAOQGS010000245         5661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7181_length_5661_cov_4.397788, whole genome shotgun sequence.
ACCESSION   DAOQGS010000245 DAOQGS010000000
VERSION     DAOQGS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_5661_cov_4.397788"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKZ52_10135"
     CDS             complement(<1..650)
                     /locus_tag="PKZ52_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNG60174.1"
                     /translation="MPHYKAPLREFDFLMAEVFKLPGFWPQLPGLGEVDWETAQEVLK
                     QAGKWCEEVLAPLNRETDEQGCQLKDGRVTTPAGFKAAYQDYAAGGWVGLGGNPAFGG
                     MGLPKSLVAAVEEMVQGSCMAFGLAPMLSAGACVALDAHASDAIKQLYLPKLYSGEWA
                     GVMDLTEPQAGTDLGLLRSKALPQDDGSYAITGSKIFITWGEQDFTDNIIHLVLARL"
     gene            927..2717
                     /locus_tag="PKZ52_10140"
     CDS             927..2717
                     /locus_tag="PKZ52_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNG60175.1"
                     /translation="MHSTSADPNSPANWRIVWVLLPYLMEFPWRVALAMALLVGAKLA
                     NVAVPIALKHIIDSLDASQALVIVPLGLLVAYGALRFLSTFCGELRDAIFARVAERAM
                     RRVTLQVFKHLHQLDLSFHLARKTGGLARDIERGTTGISFLLRFMLFNILPTLLEIAL
                     VAGILWAQFNWHYLLTVLAAVVVYAGFSIVVTEWRNRFIREANEQDNRSNTRAMDSLL
                     NYETVKYFNAEHFELAEYDKQLADWENSRLKNRLSLFVLNSGQAFIIAATVCFMMTLA
                     AYEVANKSMTLGDLVMVNAFMIQLFIPLNFLGFVYREIRQSLINIENLFKLLGEKSQL
                     KDTQGAPDLSFKQGEIRFEQVSFSYDKTRPILQDVSFAINHGQKLALVGASGSGKSTL
                     ARLLFRFYDPDAGRILIDGQAIDAVNADSLRKHMGVVPQDTVLFNDSIFYNIHYANPA
                     ASAEDVKRAAAMANLSDFIARLPQGFDTLVGERGLKVSGGEKQRIAIARILLKNPPIL
                     IFDEATSALDSTTEQSILAALNSLALKHTTLVIAHRLSTVMDADKIVVLDQGRVVEQG
                     THPELIQLGGRYWQLWQHQQEEGGNLINNP"
     gene            3840..>5661
                     /locus_tag="PKZ52_10145"
     CDS             3840..>5661
                     /locus_tag="PKZ52_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HNG60176.1"
                     /translation="MTIKAINFEDKALKRRLLNLLTFSSAMLMGQAAMAKCEFTVTTS
                     WTGGYQASLKITGEPQPVANWNAIWQFAPGTSINGAPWGDAKVTGTNPYTASSINSTN
                     TTLSTSAQLTVYMNVNGTDKNPKLSGNACGWLNKDIGAVGVSGSATVGTDNKITLKAS
                     GNDIGNDVDAFHFYYTTLQGDGEVTGYVEPGVVGPSDWTKVGVMFRGDLDANAENYAF
                     VLRPQFGSQSQFRPSKNAGYTDASERLWVQNQEINDMFFGTKHHINYVDKYPTPYRAH
                     FLQPGKWLKAVRTGSSVLTYSSDDGKCWNLRSRNDNAAFGQQAFAGFVLSSHVSNQLA
                     TAKISNVSKNQTPQADVNWDCARAKVDGDIAPPQLADWIVKPGVFGGDYWSISYTNPN
                     PVQRPATCRSKYGWDERRFTDDSTICPAEITQAPWTRTGYSLTGIWQTLPGGFGEKGL
                     GRAAYTSTPFNKKEFWLRKEIQLTSSDFNKIVFWGRWNNSASIYVNGVLATNVYSSNM
                     GDAYHYLGLRDEARSALINGTNVVTAHIECANCDSAFADFGVGRNAKLGQFYLRNQMT
                     DSTSPNYNPNYNPNSKSAKLTKVFADDVKEKALLGGSIAVR"
BASE COUNT         1442 a         1441 c         1363 g         1415 t
ORIGIN      
        1 aggcgggcca gcaccaggtg aatgatgttg tcggtaaagt cctgctcccc ccaggtgata
       61 aaaattttgc tgccggtaat ggcgtagctg ccgtcatcct gcggcagggc cttgctgcgc
      121 aacaggccca ggtcggtgcc cgcctggggt tcggttaaat ccatcacccc agcccactca
      181 ccgctgtaga gcttgggtaa atacagctgt ttaatcgcgt cgctggcgtg ggcatcaagg
      241 gctacacagg ccccggcgct gagcatgggg gcaaggccaa aggccatgca gctgccctgc
      301 accatctcct ccaccgccgc caccagggat ttaggcaggc ccatgccgcc gaacgccggg
      361 ttgccgccca ggcccaccca gcccccggcc gcatagtctt gataggccgc cttaaagccc
      421 gcaggcgtag tcacccggcc gtctttaagc tggcagcctt gttcatccgt ctcccggttt
      481 aggggcgcca gtacttcttc acaccatttg cccgcctgct ttaatacctc ctgggctgtc
      541 tcccagtcca cctcacctag gcccggcagc tgcggccaga agccgggcag cttaaacacc
      601 tcggccatta aaaagtcgaa ttcccgcagg ggggccttat agtgcggcat aactggttct
      661 cccaacgtac atgtgactgg cctgagggcg acgccctcat acccaacata aaacccataa
      721 ttaaagacta agctaattta gcggctttgc cctcccaatc gaaaaaacgt agagtgccgc
      781 ctgcgcagaa ctattaaccc gccgagttaa gtaggttcag cccagagcca taacgcatac
      841 tcccggcagc ctcactattt ttacgctcac acctccactg ttttatccat cctgctattt
      901 gcccttagga gatctctgtg cgcagcatgc actccaccag cgccgaccct aacagccctg
      961 ccaactggcg catcgtctgg gttcttttac cctatttaat ggaattcccc tggcgcgtgg
     1021 ccctggccat ggccctttta gtgggggcaa agcttgccaa tgtggccgtc cccatagccc
     1081 ttaaacacat tatcgacagc ctcgatgcca gccaggccct ggtcattgtg ccgctgggat
     1141 tattagtcgc ctacggcgcc ctgcggtttt tatcgacatt ttgcggtgaa ctgcgggatg
     1201 ccatttttgc ccgggtggcc gagcgcgcca tgcgccgggt caccctgcag gtgttcaaac
     1261 atttacacca attggattta agttttcacc tggcccgcaa aaccggcggc ctggcgcggg
     1321 atatagaacg cggcaccacc ggcatcagtt ttctgctgcg ttttatgctg tttaatattc
     1381 tgcccaccct gctggaaatt gccctggtgg ccggtatttt atgggcgcag tttaactggc
     1441 attacctgct caccgtgctg gctgcggtgg ttgtatacgc ggggttttcc attgtggtta
     1501 ccgagtggcg caaccggttt attcgcgagg ccaacgaaca ggacaaccgc tccaacaccc
     1561 gcgccatgga cagcctgctc aattacgaaa ccgtcaaata ttttaatgcg gaacactttg
     1621 aattggccga atacgataaa caattggccg actgggaaaa ttcccgctta aaaaatcgtt
     1681 tatccctgtt tgtgctcaat tccggtcagg cgtttattat cgccgccacc gtatgtttta
     1741 tgatgaccct cgccgcctat gaggtggcca ataaatccat gacgctgggg gatttggtga
     1801 tggtcaacgc ctttatgatt caactgttta tcccgctgaa ctttttaggc tttgtgtatc
     1861 gggaaattcg ccagtcgctg attaatatcg aaaacctgtt taaattactc ggcgaaaaaa
     1921 gccaactaaa agacactcaa ggagcgccgg atttaagttt taaacaaggg gaaattcgtt
     1981 ttgagcaggt gagttttagc tacgataaaa cccgccccat actgcaggat gtgtccttcg
     2041 ccattaacca cgggcaaaaa cttgccttag tcggcgccag cggctccggt aaatccaccc
     2101 tggcgcggtt attatttcgc ttttacgacc cagatgccgg gcgcatatta attgacggcc
     2161 aagccataga cgcggtaaat gccgactccc tgcgcaaaca catgggcgtg gtgccgcagg
     2221 atacagtgct gtttaacgac agtatttttt ataacatcca ttacgccaat cccgccgcta
     2281 gtgctgaaga cgtaaaacgc gcggcggcca tggccaattt aagcgacttt atcgccaggt
     2341 tgccgcaagg ctttgacacc ctggtgggcg agcgcggttt aaaagtctcc ggcggcgaaa
     2401 aacagcgcat cgccatcgca cgcatattat taaaaaaccc gccgatttta attttcgacg
     2461 aggccacctc cgccctggat tccaccaccg aacaatccat cctcgccgcg cttaattccc
     2521 tcgccttaaa acacaccacc ctagtgatcg cccaccgcct ctcgacggtg atggacgccg
     2581 ataaaattgt tgtgctggac cagggccgcg tggtggaaca aggcactcac ccagaattaa
     2641 tacagctggg tgggcgctac tggcagttgt ggcagcatca gcaggaagaa gggggaaact
     2701 tgataaacaa tccataagcg aggggctgtg caaatggatt tattcacacc gagattctgc
     2761 aagacaactc tcttcaacct ctgtaagcga ggagcttgga gagggcaatc agagatttac
     2821 gacgccataa cgctattttc agtgcttcac aaacacatgc tgaattaatg tattcaatcg
     2881 tcaaccagtt tctaacctgt attaaaaaat cgataaacat cgccagacat atccggctac
     2941 ctgaggtttt gaatgaggat ggtatcactg cccattttta ttatggcgta ctcgtttctt
     3001 aactgcacat caataaattc agcgctttcg gctgagcatt gctgctctcg gtggcgactt
     3061 aacagcctgt tgaagctcta gcttgcgagg tgtggcggcg ctaaattacg agctaggcga
     3121 ccagccaaaa cttaacttgc catcccatcg cagccaacat actcaacaag ccgccaaaca
     3181 accgaacctt ttttgctgca ggattagcgt cttaatattt tgtgtgtttg ggaggctatt
     3241 tgggatattt ccataatttt tatttcagct tcattgccac agctattagg atttcgaacg
     3301 cctccgccgt ctcactccgt atctgctcta taaatggcag ccagccagtt gcagattagg
     3361 cgagggtata aacctgtctt gtctaggcat gccacaagcc cccacctgaa ctcagaaaag
     3421 tgctttttgt tcgatttata gcggcttcct ggttatggcc acctcttaag ccatttaaaa
     3481 cctaattggg gtgtccgctt tgatagagaa tctcatgccg aaagctaaaa gcgtgacatt
     3541 catcacacaa aaagccatga ggtaaaaccg cagatatgtg tcccatgtgc cgttcgtttc
     3601 agatcctaga aggttaggaa tcgggcgcta ataacggaat tttgttctcc aaaaggaatt
     3661 tttaatcaaa aacttgagat ttaaggcagt agtcagatcg cttctggaga tcttaattct
     3721 ggagcccgag tcgatttagg cacagacgct agcaaacggt tttactgcga agcgaatagg
     3781 ggacaaaaaa gtgggaaaaa ttaacactca atcttgtaac tatcatttga gggaatttta
     3841 tgacgattaa ggctattaat tttgaagaca aagcattaaa gcggcggctg ttgaacctgc
     3901 ttacatttag ttcggccatg ttaatggggc aggctgcaat ggcgaaatgt gaatttacgg
     3961 tgaccacgag ttggacgggt ggctaccagg ccagccttaa aattacaggc gaaccacaac
     4021 ctgtagcaaa ctggaatgca atctggcagt ttgcacctgg cacatcaatt aatggcgccc
     4081 catggggtga cgccaaggtt acaggtacta acccgtacac tgcctcttct attaattcaa
     4141 caaatactac tcttagcact agtgcacaat taaccgttta catgaatgtg aatggtaccg
     4201 ataagaatcc taaactttcg ggtaatgcgt gtggttggct aaacaaagat ataggtgctg
     4261 tcggcgtatc ggggtctgca accgtcggca ccgataacaa aattacactt aaagcatcag
     4321 gaaatgatat aggcaatgac gttgacgcgt ttcactttta ttacacaaca ttacagggtg
     4381 acggggaagt tactggctac gttgagccag gtgttgttgg tccatccgat tggacgaaag
     4441 tgggggtaat gtttaggggg gatcttgatg ccaatgcaga aaattatgcg tttgtgcttc
     4501 gtccacaatt tggttctcag tcgcaattca gaccaagcaa gaatgctggc tacactgatg
     4561 cttcggaacg cctgtgggtg cagaaccaag aaattaacga tatgtttttt ggcaccaagc
     4621 atcatattaa ttatgtcgac aaatacccaa caccctatcg agcgcatttt ctacaaccag
     4681 gcaaatggtt gaaggcagta agaacaggta gctcagtatt gacctattct tcggatgacg
     4741 gaaaatgctg gaatttaaga tcacgcaatg acaacgcggc gtttggtcag caagcctttg
     4801 caggctttgt cctgagttct cacgtttcaa atcagcttgc aacggcaaaa ataagcaatg
     4861 taagtaagaa ccagacacca caggcggatg tgaattggga ttgtgctcgc gccaaagtgg
     4921 atggtgatat tgctcctcca caactggccg attggatcgt aaaaccaggc gtattcggtg
     4981 gtgactactg gtctatttcc tatactaatc ctaatcctgt tcagcgccct gcaacctgca
     5041 gatctaaata tggttgggac gaaagacgct ttacagatga ttcaaccata tgtccagctg
     5101 aaatcacaca ggcaccttgg actcgcacag gctattccct cactggaata tggcagacgc
     5161 tgccgggagg atttggcgaa aaaggtttgg gaagggctgc ttatactagt actccgttta
     5221 acaaaaagga gttttggttg cgcaaagaaa tccagttaac ttccagtgat tttaataaaa
     5281 tcgtgttctg gggccgctgg aacaacagtg ccagcatcta tgtaaatggt gttctcgcga
     5341 caaatgttta ttcaagcaat atgggcgatg cctaccatta tttgggcctg agagatgaag
     5401 cgcgaagtgc tttaattaac ggtaccaatg tggtcactgc gcacattgaa tgcgcaaact
     5461 gcgatagtgc ttttgcagat tttggtgttg ggcgcaatgc caagttaggt caattttatc
     5521 tgcgcaatca aatgacagat tcaacttcgc ccaattacaa tcctaactac aacccaaata
     5581 gcaaatctgc aaaactcacc aaagttttcg ccgatgacgt aaaagagaag gcgttgttag
     5641 gcggttcaat tgctgttcgg c
//
LOCUS       DAOQGS010000246         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7187_length_5657_cov_4.231881, whole genome shotgun sequence.
ACCESSION   DAOQGS010000246 DAOQGS010000000
VERSION     DAOQGS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_5657_cov_4.231881"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /gene="ispF"
                     /locus_tag="PKZ52_10150"
     CDS             complement(<1..261)
                     /gene="ispF"
                     /locus_tag="PKZ52_10150"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNG60177.1"
                     /translation="MNIRIGQGFDVHAFGPGDKIVLGGVVIPYSQGLVAHSDGDVLLH
                     ALADALLGAAALGDIGRHFPDSDPAYANADSRALLRMVVAKVR"
     gene            complement(258..971)
                     /gene="ispD"
                     /locus_tag="PKZ52_10155"
     CDS             complement(258..971)
                     /gene="ispD"
                     /locus_tag="PKZ52_10155"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNG60178.1"
                     /translation="MTDTPPRLWALIPAAGTGSRMQAASPKQYLPLAGKTLLEVTLGK
                     FLSRRDLAGLLLVSAADDSRAAALELPLLRCSGGASRADSVLAGIEFLLTQGAEGQDY
                     VLVHDAARPCVTTERIDALIQTVLAQGRGGLLACPVSDTLKQSDGEQLPATSCTLDRT
                     QIWQAHTPQMFRLGELKAALQQAKILGQAVTDEASALELTGVKPLLVADRRDNIKVTL
                     PEDLALAEFILQRHEEQGL"
     gene            complement(968..1255)
                     /gene="ftsB"
                     /locus_tag="PKZ52_10160"
     CDS             complement(968..1255)
                     /gene="ftsB"
                     /locus_tag="PKZ52_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNG60179.1"
                     /translation="MRYLVIALLVLLGYLQYRLWMAEGGFVDVARLNKEIARQERENA
                     ALRERNRILLAEVESLKQGVDTLEERARTDMGMVKEGETFFLFLKDNHKPQ"
     gene            complement(1337..2623)
                     /gene="eno"
                     /locus_tag="PKZ52_10165"
     CDS             complement(1337..2623)
                     /gene="eno"
                     /locus_tag="PKZ52_10165"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNG60180.1"
                     /translation="MSTIVNVTGFEVMDSRGNPTVMAEVVLACGAVGSACAPSGASTG
                     SREALELRDGDKSRYLGKGVLKAVANINDTIKPLLLGKDAINQRELDNIMLAADGTEN
                     KSKLGANAILAVSLAAAKAAAISKKVPLYAHIADINGTPGRYTMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVAAKTFADALRMGAEVFHSLKKVLSGRGLNTAVGDEGGFAPNLPS
                     NEAALQAIAEAVAAAGYTLGTDITLALDCAASEFYKEGRYHLDGDGKTFSSAEFADYL
                     ADLAARYPILSIEDGMDESDWDGWKVLTDKIGSKIQLVGDDLFVTNTKILKEGIDKSI
                     ANSILIKFNQIGSLSETLDAIKMAQDAGYTAVISHRSGETEDTTIADLAVATAAGQIK
                     TGSLSRSDRVAKYNRLLRIEAELGAKAAYKGRAEFK"
     gene            complement(2676..3530)
                     /gene="kdsA"
                     /locus_tag="PKZ52_10170"
     CDS             complement(2676..3530)
                     /gene="kdsA"
                     /locus_tag="PKZ52_10170"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNG60181.1"
                     /translation="MSASPVIQVGAIPVANHLPMVLFGGMNVLESRDMAMQVAEHYVQ
                     VTQKLGIPYVFKASFDKANRSSIHSYRGPGLDEGLKIFAEIKRTFGVPVITDVHEPHQ
                     AAAVAEVCDVIQLPAFLARQTDLVAAMAATKAVINVKKPQFLSPGQMKNIVEKFRECG
                     NEQVILCERGSCMGYDNLVVDMLGFRTMRECTGGLPVIFDVTHSLQCRDPMGAASGGR
                     RQQVAELARAGIAVGLAGLFLEAHPDPDTARCDGPSALPLAQLEPFLAQLKAIDDLVK
                     SLPALDIR"
     gene            complement(3527..5158)
                     /locus_tag="PKZ52_10175"
     CDS             complement(3527..5158)
                     /locus_tag="PKZ52_10175"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNG60182.1"
                     /translation="MTRFVFVTGGVVSSLGKGIASASLAAVLEARGLKVTIMKLDPYI
                     NVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFLRTRMTRRNNFTTGRVYETVLRKE
                     RRGDYLGGTVQVIPHITDEIKRRILEGGEGYDVALVEVGGTVGDIESQPFLEAIRQLK
                     VDMGPSNAQLIHLTLVPYIATAGETKTKPTQHSVKELRTIGLQPDFLLCRSEIPVDED
                     SRRKIALFTNVSFDCVVPLPDCDTIYRIPRLLQTHKLDAILLKHFGIEAPEADLSEWD
                     RVIEGKLHPTKAVTIAMVGKYMDLLDAYKSLIEALSHAGIRTFTQVNFNFINAEDLEE
                     QGLKLLDGADAILVPGGFGERGLEGKLMAVRHARENKIPYLGICLGMQSVVIEFARNV
                     LGWSDANSTEFNPKSSHPVIGLITEWITAEGAVETRDESSDLGGTMRLGAQECRLVKG
                     SKARAIYGSDLIRERHRHRYEVNNRYLDALRQGGLSIGGWSADDTLVEVVELPDHPWF
                     VACQFHPEFTSTPRDGHPLFTSYIQAAVAHKEAQA"
BASE COUNT         1213 a         1596 c         1642 g         1206 t
ORIGIN      
        1 ccgcaccttg gccaccacca tacgcagcag ggcgcgactg tcggcattgg cgtaggcggg
       61 gtcgctatcg ggaaagtggc ggcctatatc gcctaaagcc gcagcgccca gcagcgcatc
      121 cgccagggcg tgcagtaata catcgccgtc ggaatgggca actagccctt ggctataggg
      181 gattaccacg ccgcccagca caattttgtc accggggcca aaggcgtgga cgtcgaagcc
      241 ctggccgatg cgaatattca taagccctgc tcctcgtggc gctgcagaat aaattccgcc
      301 agagctaaat cctccggcag tgtcactttg atattgtccc gccggtcggc aaccagcagg
      361 ggtttaacgc cggtaagctc cagggcagag gcttcatcgg tcaccgcttg gccgagaatt
      421 ttagcctgct gcagggccgc tttaagctcg cctaggcgga acatttgcgg cgtgtgggcc
      481 tgccaaattt gggtgcgatc gagggtgcac gaagtggcag gcagttgctc gccgtcggac
      541 tgtttaagag tatcgctcac cgggcaggcc aagaggccgc cgcggccctg ggcaagcacg
      601 gtttggatca aggcgtcgat gcgctctgtg gtaacacaag gccgtgcggc atcgtgcacc
      661 agtacatagt cctggccctc ggcaccctga gttaacaaaa actcaatccc cgccagcacc
      721 gaatcggcgc gactggcgcc gccagagcag cgcagcaaag gcagttcaag agcggcggca
      781 cgactgtcat cggccgccga caccaacagc aaaccagcta aatcccggcg ggacaaaaat
      841 ttccccaggg taacttctaa aagggttttg cccgccaaag gaaggtattg ttttggcgag
      901 gccgcctgca tacggctgcc cgtacctgca gcgggaataa gcgcccaaag gcgggggggc
      961 gtgtcggtca ttgcggtttg tggttatctt ttaaaaacag aaaaaacgtc tcgccttctt
     1021 tcaccatacc catatccgtg cgggcacgct cctccagggt atccaccccc tgcttgaggg
     1081 attcaacctc cgccagcagt atgcgattgc gctcgcgcag ggcggcgttc tcccgctcct
     1141 ggcgggcgat ttctttgttc aaacgcgcca catccacaaa gccgccctca gccatccaca
     1201 ggcggtactg cagataaccc aaaagcacta atagggcgat taccaaataa cgcatgaacg
     1261 gggccgcaag cgaggtaaga aggtggcagc aggcataaaa aagccccaga ccaagctggg
     1321 gctttttcag acgggcttat ttgaactcag cgcggccttt gtaggcagcc ttagcgccca
     1381 gctccgcctc gatgcgcagt aagcggttgt acttggcgac gcggtcagag cgcgagagtg
     1441 aaccggtttt gatttgtcct gcggcggtgg ccacggccag gtcggcgatg gtggtgtctt
     1501 cggtttcgcc agagcggtga gaaataaccg cggtgtaacc tgcatcctgt gccattttga
     1561 tagcgtccag agtttcgctt aaggagccga tttggttgaa tttaatcaaa attgagttgg
     1621 cgatggattt gtcgatgccc tctttgagga tcttggtgtt ggttacgaac aggtcgtcgc
     1681 ccaccaattg gattttgctg ccgattttat cggtgagcac tttccagccg tcccagtcgc
     1741 tctcatccat accgtcttcg atggacaaaa tagggtaacg ggcggccagg tccgccaggt
     1801 aatcagcaaa ctcagcagag ctaaaggttt tgccatcgcc atccaagtgg tagcggcctt
     1861 ctttgtagaa ttcagaggca gcgcagtcca gggccagagt gatgtcggtg ccaagcgtgt
     1921 aaccggctgc ggcaacggct tcggcaatgg cttgcagggc cgcttcgttg gacggcaggt
     1981 tcggcgcaaa gccaccctca tcgcccacag cggtgttaag gccacggccg gagagcactt
     2041 ttttcaagct gtggaagact tccgcgccca tgcgcaaagc atcggcgaag gttttagcgg
     2101 ccactggctg aatcatgaac tcttgaatgt ccacgttgtt atccgcgtgc tcaccgccgt
     2161 tgatgatgtt catcataggc accggcatgg tgtaacggcc cggggtgccg ttgatgtcgg
     2221 cgatgtgggc gtagaggggc acttttttgc tgatggcagc ggccttagcg gcagctaagg
     2281 acacagccaa aatggcattg gcgcccagct tagatttgtt ctcggtgccg tcggcggcca
     2341 acatgatgtt gtccagttcg cgctggttga tggcatcttt gccgagcagc aagggcttga
     2401 tggtgtcgtt gatattggcc acggccttta acacgccttt gcccaggtag cggctcttat
     2461 ctccatcccg cagctcaagg gcttcgcggg agccagtgga ggcacccgac ggggcgcagg
     2521 cgctgccgac ggcgccgcag gctaagacca cttctgccat taccgtgggg ttgccacggg
     2581 aatccattac ttcaaaaccg gttacgttaa ctattgtgct catggggtct ccgttgtgcg
     2641 ttctgtcgag tgaaaggggt ttgcaaaatt attgtctagc gaatatccag ggcgggcagg
     2701 gatttcacta ggtcgtcaat ggctttgagc tgcgctaaaa agggttccaa ctgcgccaag
     2761 ggtaaagcac tggggccgtc gcagcgggcg gtgtctgggt ctgggtgggc ctctaaaaat
     2821 aaacctgcaa gacccacggc gataccggcg cgggcaagct ccgccacctg ctggcgccgc
     2881 ccaccggagg ccgcgcccat ggggtctcgg cactgtaggg agtgggtcac gtcgaagatt
     2941 accggtaggc ccccggtgca ctcgcgcatg gtgcgaaagc ccagcatgtc caccaccagg
     3001 ttgtcataac ccatacaaga gccgcgctca cacaaaatca cctgctcgtt accgcactcg
     3061 cggaatttct cgacgatgtt tttcatctgg ccggggctta aaaactgcgg tttttttaca
     3121 ttaatcaccg ccttggtggc ggccatggcg gcgaccaaat ccgtctggcg ggccaaaaac
     3181 gctggcagct gaatcacatc gcacacctcg gccacggcgg cggcctggtg gggttcgtgc
     3241 acatcggtaa tcaccggcac cccaaaggtg cgttttattt ccgcaaagat cttcagcccc
     3301 tcgtccaggc ctgggcctct gtaggagtgg atggaggagc ggttggcctt atcaaaggag
     3361 gccttaaaca catagggaat gccgagtttt tgcgtgacct gaacatagtg ctccgccacc
     3421 tgcatggcca tatcccggga ttccaatacg ttcatgccgc caaacagcac catgggcagg
     3481 tggttggcaa ccgggatggc gcctacctga attaccggag acgcgctcat gcttgggcct
     3541 ctttgtgggc gacggccgct tggatataac tggtaaacaa ggggtggcca tcccgggggg
     3601 tggaggtgaa ttcagggtga aattggcagg ccacaaacca ggggtgatcg ggcagttcga
     3661 caacctccac caaagtgtcg tcagcagacc aaccgccgat gctaaggcca ccctggcgca
     3721 gggcgtccag gtagcggttg ttcacttcat agcggtggcg gtggcgctcg cggattaggt
     3781 ctgagccgta aatggcgcgg gctttagagc ctttcaccag gcggcactcc tgggcgccta
     3841 aacgcatggt gccacctaaa tcagaggatt catcgcgggt ttcgacggcg ccttcggcgg
     3901 tgatccactc ggtgatcagg ccgatcaccg ggtggctgct tttggggttg aattcggtgg
     3961 agttcgcatc gctccagcct aatacattgc gggcaaattc aatcaccacc gattgcatac
     4021 ccaggcaaat acccaagtag gggattttat tctcccgggc gtggcgcaca gccatcagct
     4081 tgccttctaa gccccgctcg ccaaaaccgc cgggcaccaa aatcgcatcg gcgccatcga
     4141 gcagcttaag gccctgctct tctaagtctt cggcgttgat aaagttaaaa ttcacctggg
     4201 taaaggtgcg aataccggcg tgggagaggg cttcaatgag ggatttatag gcatccagta
     4261 aatccatgta tttgcccacc atggcgatgg taaccgcttt ggtggggtgt aacttgcctt
     4321 cgatcactcg atcccactca cttaaatcag cttccggcgc ttcaatgcca aagtgtttga
     4381 gcaaaatagc gtcgagctta tgggtttgta acaggcgggg aatgcggtag atggtgtcgc
     4441 agtctggcag aggtactacg cagtcaaaac tcacgttggt gaatagcgca attttgcggc
     4501 gggagtcttc atctaccggg atttccgagc ggcagagcaa aaagtccggc tgcaggccga
     4561 tggtgcgcag ctcttttacc gagtgctgag tgggcttagt tttggtctcg cccgcagtgg
     4621 ctatgtaggg caccagggtg aggtgaatta actgggcatt gctcggcccc atgtccactt
     4681 ttaactggcg gatggcctct aaaaacggct gggattcaat atccccaact gtgccgccca
     4741 cttccaccag ggccacatca tagccctcgc caccctctaa aatgcggcgt ttgatttcat
     4801 cggtaatatg cggaatcact tgtaccgtgc cgcctaaata atccccccgg cgctctttgc
     4861 gcaggacagt ttcatagacg cggccagtgg tgaagttgtt gcggcgggtc atgcgggtgc
     4921 gcaaaaagcg ctcgtaatgg cccagatcta aatcggtttc cgcgccgtca tcggtcacgt
     4981 acacctcacc gtgttgaaac gggctcatgg tgcctgggtc cacattaatg taggggtcaa
     5041 gcttcatgat ggtcactttc aagccccgcg cttcaagcac ggcggccaat gacgctgacg
     5101 caataccctt acccagtgag gacaccaccc caccggtcac aaacacaaaa cgagtcatga
     5161 aacgcttaaa accttgagta taaacatgcg cgcaggggcg ctggacattg cgcggcgggg
     5221 ccgaacacac caccgcaaca atagggcaca caggcaacag ctgcctggct gaacggggcg
     5281 gcagactagc agattcggcc tttcgcctca atcgccgcag gcgcaaagcc cgcaagcact
     5341 aaggcggtta acaggtatta gccgcttggc agacattttg ccaggccaca cagccattta
     5401 gccaaacctg tgttatgctt tgaacacctg cctttgactg agcttgtact ccctcaaagc
     5461 cttcgcaccc tctgcaccct ctcgcagaca ctcaaccccc cttcttaaaa tcacgcgacg
     5521 gttttgctct ggtgttttcg ctggcagtgt tccccaagta atttgatatt gcggcctaca
     5581 ggctgcaata tcaaattact tggggaaccc tgccagcgaa aacgccagag caaaaccgtc
     5641 gcgtgatttt aagaagg
//
LOCUS       DAOQGS010000247         5639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7218_length_5639_cov_3.849033, whole genome shotgun sequence.
ACCESSION   DAOQGS010000247 DAOQGS010000000
VERSION     DAOQGS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7218_length_5639_cov_3.849033"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKZ52_10180"
     CDS             <1..551
                     /locus_tag="PKZ52_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60183.1"
                     /translation="LVDNLANSLALGITNRSASLLGQTVGGDVKSGLTLFQGEPVLRL
                     RLPLDRAKASLSRALESEGFVKWGQADDGLLYLEGYADQESPPGWFARTILRRKAQAP
                     AKPALPWDSAVKHLDPAAAPAFEGIAEAAFGAPHKKAEGYWVRLLPHGSEQLVLIRSV
                     RGLPLEPLEAKARLSVLRRNLM"
     gene            555..1310
                     /locus_tag="PKZ52_10185"
     CDS             555..1310
                     /locus_tag="PKZ52_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNG60184.1"
                     /translation="MRFASLGSGSQGNATLVSCDGDTLLVDCGFSLRELHSRMERLGA
                     APADLSAVLVTHEHSDHIRGIGPLARKYRIPVVMTAGTRASGRLGPLPLERIIEPGVA
                     FELGGFEILPVAVAHDAAQPSQFVFHRAGKKLGLLTDLGSITASVIDAYQACDALLVE
                     ANHDPVMLACGPYPASLKRRVAGQWGHLSNAQTQSLLQQLDISRLQHLVIGHISQTNN
                     SICRAAAALEGILPHFPIHYACQNQGFDWLTLQ"
     gene            1324..2034
                     /locus_tag="PKZ52_10190"
     CDS             1324..2034
                     /locus_tag="PKZ52_10190"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNG60185.1"
                     /translation="MQRKDELYKGKAKSVFSTDDPDKLILLFRNDTSAFDGKKVEQLD
                     RKGMVNNKFNAFIMGKLQAAGIPTHFEGLLSETESVVKSLTMIPVECVVRNIAAGSIC
                     RRLGVQEGMELNPPTFEFFLKNDALGDPMINDYHIRSFGWATEAQVDTMKALTFKVNA
                     VLKELFLAGGMLLVDYKLEFGVYKGEVILGDEFSPDGCRLWDVETREKLDKDRFRQNL
                     GNVIESYELVGRRLGLEF"
     gene            2443..2712
                     /locus_tag="PKZ52_10195"
     CDS             2443..2712
                     /locus_tag="PKZ52_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG60186.1"
                     /translation="MEDRRLYERTQASFKVILTHPSIGTIMGTARDISDGGAQVMIEN
                     HALPPVGTVLNVLFKKTVGQVNEQPVPMKIVYAQRNIIGLKFEGR"
     gene            complement(2761..3483)
                     /locus_tag="PKZ52_10200"
     CDS             complement(2761..3483)
                     /locus_tag="PKZ52_10200"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNG60187.1"
                     /translation="MSTLSNLKLYATYPHACSYLEGQEATTLFIDPQARVDHALYSHL
                     SDLGFRRSGDHIYRPRCQTCKACIPVRIPVLQFRATRQQKRCWARNQDLTIIVSQQLN
                     LPLYYPLYARYISERHADGDMYPPNEEQLVNFLKPAVDEGTDYLEFWLQDELIAVAVS
                     DRLAQGLSAIYTFYSPAPEHQGRSLGVMAVLFQIARARQLALPYVYLGYWIKNCRKMA
                     YKNHYRPLELLIEQVWVPTGLE"
     gene            complement(3461..4183)
                     /gene="aat"
                     /locus_tag="PKZ52_10205"
     CDS             complement(3461..4183)
                     /gene="aat"
                     /locus_tag="PKZ52_10205"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNG60188.1"
                     /translation="MLPPWLRADKRWFPPTSAALAEPNGLLALGGDLSPTRLINAYQR
                     GIFPWYSEGQPILWWSPDPRVVIYPGEAHISSSLKKFLRKSPWQVRIDQTFNEVTRQC
                     AAPRRDSHGTWLLDDMQQAYGQLHNLGVAHSVEVWGEDELLGGLYGVSLGRVFFGESM
                     FSRAENGSKVALVALSHLLKQADFALIDCQVGNPHLFSMGAVQLPRAKFEALLQQHAG
                     APEGPWSYWSTLSFPCEYFIQP"
     gene            complement(4187..5137)
                     /gene="trxB"
                     /locus_tag="PKZ52_10210"
     CDS             complement(4187..5137)
                     /gene="trxB"
                     /locus_tag="PKZ52_10210"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNG60189.1"
                     /translation="MSTPQHHRLIILGSGPAGYTAAIYAARANLKPVVITGMQQGGQL
                     TTTTEVENWPGGVHDLQGPDLMVQMQEHAERFETQIIFDHINEVDLQNRPFRLKGSEE
                     YTCDALIIATGASAQYLGLESESAFQGRGVSACATCDGFFYRDQTVMVVGGGNTAVEE
                     ALYLANIAKEVILVHRRDFLRSEKILQDRLMDKAKNGNIKIMWNSQVDEVLGDEKGVN
                     AVRIKSTSDGSTQDVPVNGLFIAIGHKPNTEIFAGQLDMHNGYIKVKSGTEGNATQTN
                     VTGVFAAGDVADHIYRQAITSSGSGCMAALDAERYLDALK"
     gene            5413..>5639
                     /locus_tag="PKZ52_10215"
     CDS             5413..>5639
                     /locus_tag="PKZ52_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNG60190.1"
                     /translation="MRQTETTQRPQQATRTLAGKMLREGLLILLATACVFLLMALSTY
                     SQKDPGWSSTGLNEVLSNAGGPFGAWLADVF"
BASE COUNT         1234 a         1493 c         1609 g         1303 t
ORIGIN      
        1 ggctggtgga taacctggcc aacagcttag cgctgggcat taccaatcgc tcggcgtcac
       61 tattggggca aaccgtgggc ggcgacgtga agtccggctt gacgctgttc caaggcgagc
      121 cggtactgcg cctgcgcctg ccgctggatc gcgccaaggc cagtttaagc cgcgcccttg
      181 agtctgaagg ttttgtgaag tggggccagg cggacgatgg cctgttgtat ttggaaggct
      241 acgccgatca agaatccccc cccggctggt ttgctcgcac gattctgcgc cgcaaagccc
      301 aggcgcccgc caagcctgcg ctgccgtggg atagcgctgt taaacacttg gatccagcag
      361 cggcgcccgc ctttgagggt atcgctgagg ccgccttcgg ggcgccacat aaaaaagctg
      421 aaggctactg ggtgcgcctg ttaccccatg gcagcgagca attagtcttg atccgcagcg
      481 tgcgcggcct gcccctggag cccttggaag ccaaggctcg tttgagtgtt ctgcgccgga
      541 acctcatgta atccatgcgt tttgcgtccc tgggcagcgg tagtcaaggc aatgcaacct
      601 tagtgagttg tgatggcgac acgctgttgg tggactgcgg ttttagcctg cgggagttgc
      661 atagccgcat ggagcgcctg ggcgcggctc ctgccgacct tagcgcggtg ttggtcactc
      721 acgaacattc agaccatatt cgcggcatcg ggcctttggc ccgcaagtac cgcatcccgg
      781 tggtgatgac ggcgggcacc cgggccagtg gccgtttagg cccgctgccc ctggagcgta
      841 ttattgaacc tggggtagcg tttgagttgg gcggctttga gattctaccg gtggcggttg
      901 cccacgatgc ggcacagccc agccaatttg tattccaccg ggcgggcaaa aaattggggc
      961 tgctgacgga tctcggcagt attaccgcca gcgtgatcga tgcctaccaa gcctgcgatg
     1021 cactgttggt ggaggccaac cacgatccgg tgatgctcgc ctgcgggccg taccccgcct
     1081 cccttaagcg ccgagtggcc ggccagtggg ggcatttgag caatgcccaa acccagagtt
     1141 tactgcagca gttggatatc agccgcctgc agcatttagt gattggccac atcagtcaaa
     1201 caaacaacag catctgccgc gcggcggccg cgttggaagg tatactgccg cactttccta
     1261 ttcactacgc ctgtcaaaat cagggctttg actggctgac acttcagtag caagaggttt
     1321 cttatgcaac gtaaagacga gttatacaag ggtaaagcca aatcggtttt ttctacggac
     1381 gacccggaca aactgatttt attattccgc aacgacacct cggcgtttga cggcaaaaag
     1441 gttgagcagc ttgaccgcaa aggcatggtg aataacaagt ttaacgcctt cattatgggc
     1501 aaattacagg cggcgggtat ccccactcac tttgaaggcc tactgtcgga gaccgaatcc
     1561 gtggtgaaat ccctcaccat gattcctgtg gaatgtgtgg tgcgcaacat cgccgcgggt
     1621 tccatttgtc gccgtttggg cgtgcaagag ggcatggaat taaacccacc cacctttgag
     1681 ttcttcctaa agaacgacgc cctgggcgac cccatgatta acgactacca catccgctct
     1741 tttggctggg ccacggaagc gcaagtggat accatgaagg cccttacctt caaggtaaac
     1801 gccgtactta aagagttgtt tttggccggt ggtatgttac tggtggacta caagcttgag
     1861 tttggcgttt ataagggcga ggtgattttg ggtgatgagt tctctcccga tggctgccgc
     1921 ctgtgggatg tggaaacccg cgagaaacta gataaggacc gcttccgtca aaacctcggc
     1981 aatgtgattg aatcctatga gttagtgggc cgccgtttgg gcttggaatt ctgattctcg
     2041 ccgccttcag taccggttaa ttcccccgag aacagcacct ttaaggtgct gtttttcgtt
     2101 caggccagca ctaattcaag gcacacacgg gtgaattttc cacaagtgat aaagttcaag
     2161 aggttttacc caaaaaaatg ctaaaaaaat tctctctact cgattgacta ttttaagttg
     2221 ttgaaattaa aagtattttt taatttgtat aaaagcccac caacacccca gaattcagaa
     2281 aaaatgcgct ttggcgccac ttactcataa tgcgtgcaca gacttatcca cagactgtgt
     2341 ggataaatag tcctgtttgg caatgaccat tagtcgggcc ttttggtgaa gttgccgagc
     2401 gcctgctaag gttaggggag atcccaacta ggagcagaaa ggatggaaga tcgtcgtctc
     2461 tatgagcgca cccaggcctc gtttaaagtc attctcaccc acccatccat aggcaccatc
     2521 atgggcacgg cccgcgatat ttccgacggc ggcgcccagg tgatgattga aaatcacgcc
     2581 ctgccgccag tgggcacagt gctgaatgtt ttatttaaga aaaccgtcgg gcaggtgaat
     2641 gagcagccgg tgcctatgaa aatcgtctat gcccagcgca atatcatcgg tttgaagttt
     2701 gaaggccgtt aagcttggcg ccacctaggc gcaggtatcc gcttggacga agctgtcggg
     2761 ctattccagc ccggtgggca cccatacctg ctcgattagc agttccaagg gccggtagtg
     2821 gtttttgtag gccatcttgc ggcagttttt gatccagtag cccaagtaca cgtaaggcag
     2881 cgccagctgc cgggcgcggg cgatttgaaa caacacggcc attaccccaa ggctgcgccc
     2941 ctggtgctca ggcgccgggc tgtaaaaggt gtaaatggcg gagagcccct gggcgagcct
     3001 atcgctcacc gccactgcaa ttagctcatc ctgcagccaa aactctaagt aatctgtacc
     3061 ttcatccacc gccggtttta aaaaattcac caattgttct tcgttgggcg ggtacatatc
     3121 gccatcggcg tggcgctcgc tgatatagcg ggcgtagagg gggtagtaga gggggaggtt
     3181 gagctgttgg gaaacaataa tggttaaatc ctggttgcgc gcccagcagc gtttttgctg
     3241 gcgggttgct ctaaattgca acacggggat gcgcaccggg atacaggcct tacaggtttg
     3301 gcagcggggg cggtagatgt gatcaccact gcggcgaaag cctaaatccg acagatggct
     3361 atagagggcg tgatccaccc tagcctgagg gtctataaat agcgttgtcg cctcctgccc
     3421 ctctaaataa ctacaggcat ggggataggt ggcgtacagc ttaaggttgg ataaagtact
     3481 cacagggaaa actcagtgtg ctccagtatg accaagggcc ctcgggcgcg ccggcgtgct
     3541 gctgcaagag ggcttcaaat ttagcccgtg gcagctgcac agcgcccatg gaaaataaat
     3601 gaggattgcc cacttggcaa tcaataaggg caaaatccgc ctgcttaagc agatgactta
     3661 gggccaccag tgcaacttta gagccgttct cggcccggct aaacatggat tcgccaaaaa
     3721 atacccgccc caggctcacc ccataaagcc cgcccaataa ttcatcttcg ccccagactt
     3781 caacgctatg ggcaacgccc aggttgtgta attgcccata ggcctgctgc atgtcatcta
     3841 gcagccaggt gccatggcta tctcgcctgg gggctgcgca ctggcgcgtt acctcgttaa
     3901 aagtttggtc tatgcgaacc tgccaggggc ttttgcgcag gaattttttt aggctgctgg
     3961 agatatgggc ttcgcctggg tagatcacca cccgaggatc cgggctccac caaagaatag
     4021 gctggccttc gctataccag gggaaaatac cccgttgata ggcgttgata aggcgagtag
     4081 gggagagatc ccccccgagg gctaagagcc cgttgggctc tgcaagggcg gcgctggtgg
     4141 gagggaacca gcgtttgtcc gcccgcagcc aaggggggag catggcttac ttgagggcgt
     4201 cgaggtaacg ctcggcatct agggcggcca tacagccgga gcctgatgag gtgatggctt
     4261 gacggtagat gtggtcggcc acgtcgcctg cggcaaatac accggtgaca ttggtttgcg
     4321 tggcattgcc ttcggtaccg cttttcacct tgatgtagcc gttgtgcatg tccaactggc
     4381 cggcaaaaat ttctgtgttg ggtttgtggc cgatggcgat aaacaaacca tttaccggta
     4441 catcttgggt gctgccatcg ctggtgcttt tgatgcgcac ggcgttaacg cctttttcat
     4501 cgccgagcac ttcatccacc tgactgttcc acatgatttt gatattgccg tttttagcct
     4561 tgtccatcag gcgatcctgc aggatttttt ctgaacgcag gaaatcacgg cggtgtacca
     4621 aaataacttc tttagcgatg ttggccaggt acagggcctc ttccacggca gtattgccgc
     4681 cgcccaccac cattacggtt tgatcgcggt aaaagaaccc gtcacaggtg gcgcaggcgc
     4741 taacaccgcg gccttgaaag gcagattccg actctaaacc caggtattga gcggaggcgc
     4801 cggtggcgat gatcagtgca tcgcaggtgt attcttcgct gccttttagg cggaaggggc
     4861 gattttgcag gtcaacttcg ttgatgtggt caaaaatgat ttgggtttca aagcgctcgg
     4921 cgtgttcttg catttgcacc atcaaatccg gcccttgcaa gtcgtgcacg ccgccgggcc
     4981 agttttccac ttcggtggtt gtggtaagtt gaccaccttg ttgcataccg gtgatgacca
     5041 ccggtttaag gttcgcgcgg gcggcataaa tggcggcggt gtaaccggcg gggcctgagc
     5101 ctaaaataat caaacggtga tgctggggag tactcatatt cgcttcctaa actgaagagc
     5161 tgaggtaaat agctggatgc tgcgggggat aaattctggt gcatgggggc ggcatagtct
     5221 agagaaactt atggtcatct gccacgactt caagcagtaa aatgtacgat attttgccgc
     5281 tgccagatcg tttttgcgcc ccggcctgtg cgccgttaca gcgcgtttag cgcgcaaatc
     5341 aggttagaat aaaaacccat caggagcctg cgcccattgg ctgccgccag ctcactttaa
     5401 taaggtaatc atttgagaca gactgaaacc acacagcgac cccagcaggc cacgcgcacc
     5461 ctagcgggca aaatgctgcg tgaaggcctg ttaattctgc tggccaccgc ctgtgtgttt
     5521 ctgctcatgg cccttagcac ctacagccaa aaagacccgg gctggtcctc caccgggcta
     5581 aatgaagtgc tctccaacgc aggcggcccc tttggggcat ggcttgccga tgtgttttt
//
LOCUS       DAOQGS010000248         5636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7221_length_5636_cov_5.250851, whole genome shotgun sequence.
ACCESSION   DAOQGS010000248 DAOQGS010000000
VERSION     DAOQGS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7221_length_5636_cov_5.250851"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKZ52_10220"
     CDS             <1..924
                     /locus_tag="PKZ52_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNG60191.1"
                     /translation="ENTLPSMTDGRQVKFLFLPEGQDPDSLVRQIGKERFEQQIEQAI
                     PLEDYFFRTLAEGLNMHSMEGRARFSKLAAPQLHRLPSGVYRELMFQQLSRHTGLSLD
                     VLQELIHTPQEPAAPPVEVRSKPAAPLTATEGPSQREPQPFEPPPLVNSRARPVFQTI
                     KITPARMAILLLLEHPELTKTLDGQWRAEPAEADEQLLEQLITNIAQHQLQSFHSIMG
                     FWAGEYGVTAQKELTQMVSNQFLGAVKKLSAFDAEQELRDAFKKLATQAQQRQLRTEL
                     AQLQTIPLDALDEAQKNRLRELLKIKLLSRQ"
     gene            1087..2928
                     /gene="rpoD"
                     /locus_tag="PKZ52_10225"
     CDS             1087..2928
                     /gene="rpoD"
                     /locus_tag="PKZ52_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNG60192.1"
                     /translation="MSENQQSRIRDLINRGREQGYLTYGDVNDHLPEDISDPDQVEDI
                     IQMINDMGIRVYETAPDADELLMQDTESNTDEIAAAEAAAALAAVETEVGRTTDPVRM
                     YMREMGTVELLTREGEIAIAKRIEEGLRELMHALAYWPETTERLIAEYDLIEKGERRL
                     TDVIAGWLDPVETIAPEPVAELPPMAEKVVGAELEEEEETVSEEEEEADTGIDPAYAK
                     ERFDGLRAQLEKTKAAISKHGRQSKAAGKELDALGDVFKFFKLPPRQFDPIYNAARDV
                     LNQVRAHERDIMMQCVRYGKMPRKNFIKEFPGNETNVEWIATATKKKKDYTESLRGVS
                     DTVIASQNKLKEIEDTTGLDLSTIKEINRRMSIGEARARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELGVRMEMPEDKVRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDVSIVSPIESATTQGLMEATKAVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSDHLRGFLDD"
     gene            3011..3087
                     /locus_tag="PKZ52_10230"
     tRNA            3011..3087
                     /locus_tag="PKZ52_10230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3045..3047,aa:Ile,seq:cat)
     gene            complement(3817..4023)
                     /locus_tag="PKZ52_10235"
     CDS             complement(3817..4023)
                     /locus_tag="PKZ52_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNG60193.1"
                     /translation="MSKGTVKWFNADKGFGFITPENGSKDLFVHHSEIQAGGGYATLN
                     EGQAVEFEVGQGQKGPCANKVRAV"
     gene            complement(4547..4942)
                     /locus_tag="PKZ52_10240"
     CDS             complement(4547..4942)
                     /locus_tag="PKZ52_10240"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNG60194.1"
                     /translation="MPDLIIFDGLCHLCAGSVQFILKRESRPHYYFTPVQSATGAQVM
                     LKHGLDPLDVKTFVVIEDDRAYVRSDAALCIARRLRMPWRLLVVLRLVPRRIRDWGYD
                     LIARNRYRWFGQRNHCWLPNEAYANRFIE"
     gene            complement(5243..5602)
                     /gene="ribA"
                     /locus_tag="PKZ52_10245"
                     /pseudo
     CDS             complement(5243..5602)
                     /gene="ribA"
                     /locus_tag="PKZ52_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
BASE COUNT         1552 a         1502 c         1343 g         1239 t
ORIGIN      
        1 gaaaacactc tgccgagcat gaccgatggc cgccaggtga agtttttatt tttacccgaa
       61 ggccaggacc cggacagcct ggtgcggcaa atcggcaagg agcgcttcga gcaacaaatc
      121 gaacaagcta ttcccttgga ggattatttt tttcgcaccc tcgccgaagg cctcaacatg
      181 catagcatgg agggccgagc gcgttttagt aaattggccg ctccgcagct gcaccgctta
      241 ccatccgggg tttatcggga gctgatgttc cagcagctat cccgccacac cgggctaagc
      301 ctggatgtgc tgcaggaatt aattcacacc ccccaagaac cagcagcccc ccctgtagaa
      361 gtgcgcagca aaccagccgc cccacttacc gccacagaag gcccaagcca gcgggagccg
      421 cagccattcg agccgccacc gctagtcaac agcagggcgc ggccagtatt tcaaaccatt
      481 aaaatcactc ctgcgcgcat ggcgattttg ctgttgctgg agcatccgga actcaccaaa
      541 accctcgatg gccaatggcg cgccgaacct gccgaagcgg atgaacaatt gctggagcag
      601 ttgatcacca acatcgccca acaccaattg cagagctttc acagcatcat gggtttctgg
      661 gcgggagaat atggtgtaac ggcacaaaaa gaactcaccc agatggtcag caatcaattt
      721 ttaggcgcag tcaaaaaact ctctgccttt gatgccgagc aggaactgcg cgatgccttt
      781 aaaaaacttg ccacccaagc tcaacagcgg cagctgcgca ctgaacttgc ccaactgcaa
      841 acaatcccac tggacgctct tgatgaggca caaaaaaatc gcctgcggga gctgttgaaa
      901 atcaagctgt taagccgcca ataggccgcg cggccacccc tgtacaaacc caaagcagca
      961 aggcttagca tttgaatacc agcacaaacc aaagccctcc gcttgtaatt tctggtgagc
     1021 gccaccagat accgttatac tagttggctt tatttcttcc acacataccg gggacgcggg
     1081 aaattaatga gcgaaaatca acaatcgcga atcagagact tgatcaatcg tggccgtgaa
     1141 cagggttact taacctacgg cgacgttaac gaccacctgc ccgaagacat ctccgacccg
     1201 gatcaggtcg aagacattat tcaaatgatc aacgacatgg gcatccgcgt ctacgagacc
     1261 gccccggatg ccgatgaact cttgatgcaa gacaccgaga gtaacaccga cgaaatcgcc
     1321 gccgccgaag ccgccgctgc cttagccgct gtggaaaccg aagtgggccg caccaccgac
     1381 ccggtgcgca tgtacatgcg tgaaatgggc accgtggaac tgctcacccg cgaaggcgaa
     1441 atcgccatcg ccaaacgtat cgaagagggc ctgcgcgaac tcatgcacgc cctggcctat
     1501 tggccggaaa ccaccgagcg ccttatcgcc gaatacgacc tgattgaaaa aggtgagcgc
     1561 cgcctgacgg atgtgatcgc cggctggctg gaccctgttg aaaccatcgc gcccgaaccc
     1621 gttgccgagc tgccccccat ggcggaaaaa gtggtaggcg ccgagcttga agaggaagaa
     1681 gaaaccgtct cggaggaaga agaggaagcc gataccggta tcgacccggc ttacgccaaa
     1741 gagcgctttg acggtttgcg cgcgcagctg gaaaaaacca aagccgccat cagcaaacac
     1801 ggccgccaaa gcaaagcggc tggcaaagaa ctggatgccc taggcgatgt gtttaagttt
     1861 tttaaattgc ctccgcgtca gttcgaccct atttacaacg ccgctcgcga cgtgctcaac
     1921 caagtgcgcg cccacgagcg cgacatcatg atgcaatgcg tgcgctacgg caaaatgccg
     1981 cgcaaaaact ttattaaaga attccccggc aatgaaacca acgtcgagtg gatcgcaacc
     2041 gccaccaaaa agaaaaagga ctacaccgaa tcgttgcgcg gcgtaagcga tacagtcatt
     2101 gcctcacaaa acaaattaaa agaaattgaa gacaccaccg gccttgatct ttccaccatc
     2161 aaggaaatta accgccgcat gtccatcggc gaagcgcgcg cccgccgcgc caaaaaagaa
     2221 atggtggaag ccaacctgcg tttggtgatt tccatcgcga aaaaatacac caaccgcggc
     2281 ctgcaatttt tggatttgat tcaagagggc aatattggcc tgatgaaagc ggtggataag
     2341 tttgaatacc gccgcggcta taaattttcc acctatgcca cctggtggat tcgccaagcg
     2401 atcacccgct ccatcgccga ccaagcccgc actattcgta tcccggtgca catgattgag
     2461 actatcaaca aactcaatcg tatttcccgc caaatgcttc aagaaatggg ccgcgaacct
     2521 acaccggaag aattgggcgt acgcatggaa atgccggaag ataaggtgcg caaggtactg
     2581 aaaattgcca aagaacccat ctccatggaa acccccatcg gtgacgatga agactctcac
     2641 ttaggggatt ttatcgagga cgtatccata gtttccccca tcgaatccgc gacgacccag
     2701 ggcttgatgg aagccaccaa agcggtactg gcgggtttaa ctgcacggga agccaaggtg
     2761 ttgcgcatgc gctttggtat cgatatgaat accgaccaca cccttgagga agtcggtaaa
     2821 caatttgatg taacccgtga gcgtattcgt cagatcgaag ccaaggccct gcgcaaactg
     2881 cgccaccctt ctcgctcgga tcacctgcgc ggctttttgg acgattaatc gcgggcaagg
     2941 ctagccatgc ccaggggcgc agattataat gcgccctctt ttttagaccg taattaacac
     3001 gtctttttaa gggcctatag ctcagttggt tagagcgtcc gactcataat cggcaggtcc
     3061 caggttcaag tcctggtggg cccaccaaac acaaaagcct aacccgcaag ggttaggctt
     3121 ttttattggc ggcaaagttt tacttagcta gattattaat ccatctaaac ctattgcaac
     3181 taggctttta acgtcttctt ttgcgttggc ataagtgtgt agcccaccaa aacctggcca
     3241 ttagctatag gcttactaat tggtgctgct cgtcagcaac ttcatctttt accagggttt
     3301 taaattggcc attaatacaa atagcagagg cttttactac ctaggcacct gctatgaata
     3361 ctcatcattt cagacacgcc gtaaatacat ccttgtaggc tcgtcaaaaa gatccctctt
     3421 tttgacggtc tgaaacgatg tgtattcatt gcttccgcac agttagcatg gtgaactttt
     3481 tggatgtcgc gcttaatctt tgcaacaact cgttttctat ttaacaaaca gcaataagaa
     3541 aaaataattt ttgcgctcgg cagacggatg cacaaataaa ttgtatcgcc gcgaaagtta
     3601 aacacatgca gccaaaacga aaaaaggaat cgccaacagg cgggagagcc gacccaaata
     3661 tagctaaagt tttttagcga agaatatcaa tctagttaga ttaagatgaa ttgcgaggat
     3721 ctggggaggg ataccttgca cgcgagcgcc tgcaaggcga aacaaaggag tttacagcgt
     3781 tagatactgt tcactccgca ctatgtatct gtggtattaa accgcgcgaa ctttatttgc
     3841 gcaagggcct ttttggcctt ggccaacttc aaactctacc gcctggcctt catttaaggt
     3901 ggcgtaacca ccgccagctt gaatttctga gtgatgaaca aacaaatctt tgcttccatt
     3961 ctctggtgta atgaaaccaa aacctttgtc tgcattaaac cacttaactg tacctttact
     4021 catattactg tctactttgt gggtgaactt aaaatgaccc taagggttat caccattgcc
     4081 gtttagggtt ttaggaaaag acctggagat gcgcaaacca attgaaagct aaagtatcta
     4141 ccgcattaac ggccgacagc gctaataagt catccggata cttaaatatt gtctccaaca
     4201 gctactgatt taacgccatg ccaggcgata tagcaaggaa ttaattctgc gctggcatgc
     4261 acctaattga caaattagaa ataaacgatc tcagcacacc gataaaatag tcgataaaaa
     4321 cccaccatca attacagcga gctattagat agacccagtc gatcacttgt gacacccgta
     4381 ccccctggtg tctattttaa atgtgcggcc tatttcggca ttaggttgcc aaggatggca
     4441 tattggtcat aaatcgcacc caaagtgatg atttgacact tttaaagaaa aacaattttt
     4501 ccaagctctt tttggcttgc ctggtcggga ctagccttaa accgcattac tcaatgaacc
     4561 ggttagcata tgcctcattc ggtagccaac agtgattgcg ctgcccgaac caacgatagc
     4621 gattgcgcgc gatcaggtcg tagccccaat cgcgaattct gcggggtacc aggcgaagca
     4681 ccaccaacag gcgccagggc atgcgcaggc ggcgggcaat gcacagggcc gcgtctgagc
     4741 gcacataggc acggtcatct tcgatcacaa caaaggtttt tacatccaga ggatcaagcc
     4801 cgtgcttgag catgacttgc gcaccggtgg ccgactgcac tggggtgaag taatagtgag
     4861 gcctagattc gcgcttgagg ataaattgca cgctgccagc acataggtgg cataggccat
     4921 caaaaataat gagatcgggc atttgcaagt ctcaaccggg taatttgaaa aattggcccg
     4981 acacaattat ttcaatctgt tttctgttat gggtcttaaa ccgcacacaa ttaagtgccg
     5041 cctaataaac acctagtcaa taatccaggc taaaaccttg aggccaacaa cacagcagcc
     5101 agaaaaaaca gctagagaat caaccattac tgggctggca gaattttacc gcgctaacat
     5161 ctcacacaaa aagctataaa acagggtaat tgcagcgagt tgcttcacca agcgagtatt
     5221 gataagtaga tcgaggaatt aactattccc ccaccaacat atgcccgagc ttatcttttt
     5281 tggtatccaa ataatggtgg ttgtgggaat tgcgaccggt ttcgtgggcc aggcgggttt
     5341 caatttcaat gccgagggct tcgagggctt taattttttt ggggttgttg gtgagcagtt
     5401 ttacccgttt gatattcagg tgcgcaaaca tagggccgag catggagtag tcgcgcaggt
     5461 cagcggcaaa ccctaaatgt tcgttggcct ccaccgtatc ggcgccttga tcttgcaggt
     5521 gataggcgcg aattttattg agcaggccaa tgccgcggcc ctcttggcgc aaataaaaaa
     5581 tggcgccgct gcccgccacg gaactcaccg cccttcacgc ctgacccgcc gtgcac
//
LOCUS       DAOQGS010000249         5629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7239_length_5629_cov_4.084141, whole genome shotgun sequence.
ACCESSION   DAOQGS010000249 DAOQGS010000000
VERSION     DAOQGS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7239_length_5629_cov_4.084141"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..3584
                     /locus_tag="PKZ52_10250"
     CDS             591..3584
                     /locus_tag="PKZ52_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG60195.1"
                     /translation="MKANPIQSQSTFNRLLLTAAISTVFTLLGSPAWSAQAADADKKP
                     DDKVTETKESKKGSKSPVRLQSVSVKAKKNTKTLETKHDTPTAISVVTGETLNREIAQ
                     DYQAISKRLSNVTFNQNNSRGASLSIRGIGKRGFNEVQDPSVLVIQDGASFGLTALGN
                     FDFYDIEAVESYRGPTGTLGGKGGSAGQVNVITKQPSFKASSDFSIAYGSWDTLVVRA
                     ASGGPIIDDLLAWRGALIVDKGEGYFRNTFGYDTNNTQYNKNRAAGRIQLLLTPSEEL
                     SVRVSYDRQPKAPQLENGLTIRKDQPEYYEGFSFADYVAGNYTGVQPSPTDPNGTTPR
                     AKLYGYKSLVSADPTNPNSTIKKEQWNPGRPWFTNRPSPENPGHNYDYNAEYVGTDDN
                     PNIHFNEQQGQYLSTGGWSGEVSWLRDDFKLTSISAWRSFYFDAHNDEGTSFDISKHG
                     GGSVDFHQYSQEFRIASTPGRTLDYTAGIFLYKSDTDVQSRTSWGSDAGAWFANTGQY
                     NTLDRNAGVNRGAGLALLKDSLEDVFRKGDIWVETKSQAVFGQIDWHWSEAATLTAGL
                     RLGREDRTTEDALVLVNYGAANWLNPVKINTFNLGGFASTTTAVAASGNAPAIPVGSL
                     GRLEYQLDPATNKKIQVLINDNTAAQLQLADQAANRYYGAAINANTPGSAYNSLTAAQ
                     KSQIAAAKALRNTQIGQLIPNVKSSYEDNLVTSNLSQRYKFNEQITGYVTWQHGEKPG
                     TAFNITGVPSPVKAETTNAFELGVKTFLFNETLTLNADIFRLNIDDYQQAIRTVDTFA
                     TQSALDNLDPNLTGDALKNAQNAATVYITSQGNVPKVTVQGLEVDGAYTGVPHTTIRF
                     SGAYNDARYGEFTNSPKPDELAYLKEPFIDKSNELLPGAALVTFNVGAEYRIPFLQAE
                     FHTSFNTSYTSDFNSGDTFSKYADIDGYSITDTSLGFASKDGKYDVSLVVKNVLDEHP
                     HEVAWTTYTPYIHRRWAGLVFDSHF"
     gene            3895..5355
                     /locus_tag="PKZ52_10255"
     CDS             3895..5355
                     /locus_tag="PKZ52_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNG60196.1"
                     /translation="MNKPISFFHALAFTALALPLAVQAASLQQADATLKAKATTSLTF
                     KGTGAWYQFGQAPAPTLAWPKFDVSSYQADINFEKTAGRVQITRKQSFEPGRNRPAPT
                     EQKADQYISDTIAWNLATTPPANNAPAATAQPAALEERKAEIISTPQGFIRAALANNA
                     TSKPTKNGIEVNFTLDKKYQYKGIINDKNLVESVQTWIDNPVLGDTLYETTFADYQER
                     SGVQFPARISRKLGGHPILDISVSEVQPNGNVSIAVPAEVTAAPPAITVTSTKLAEGV
                     YYLTGGTHHSVAIEQADHVVLVEAPQHEARSLAVIAKVKEILPTKPIKYLINTHAHFD
                     HLGGVRTFVDAGATIVTHKDNQAYYQKIWQQPHSLNPDKLAQSKKTPKFATFSAKHTL
                     GDSTRKIELYPLTGNSHNDAFAFIYLPNEKILIEADAYTPLAQGVPVPANVNPYSQNL
                     YELIQSQKLDVAQIAALHGPGVVTLADLKTYIGLKS"
BASE COUNT         1632 a         1457 c         1189 g         1351 t
ORIGIN      
        1 cgagctccgc taaaacaaga aaatccacgc attttcgcca gttttagcgg agcgaaggca
       61 aaatagatca ggaagatcta ttttgccgcc gggttaatag ctagtagctg ggcatcaggc
      121 acaaaaatta aatgtgccga ctcatgacaa ttttacccgc gctaattacc tacagaaact
      181 gcagattaaa acctcagctg gtcacagcaa ggcataactg agaattttag gattattcct
      241 gcagcacagc tatcagctct taagcgccct agcctctgca ataacgccat ctcttataaa
      301 atccactctc cacatttata tccagccgct tatatgtcaa caaggcctgt tagattgcca
      361 acaggcaaac aggcctcttt catacctaag ccaaattatt aacttctcat ataacaacac
      421 tcatgcaaaa aaaacagaaa taataggtta attaaaaaca tctgcctgca ccaacacaaa
      481 cacaatacct gctataaact gcaggatatt ttttatttaa tcaaataaaa atctggcaga
      541 aacattgctc acgaggaatg aattttaatt tcaaccagcg cgagcccagc atgaaagcca
      601 acccaattca gtctcaatcc acttttaacc gcctgttatt aaccgccgca atttctaccg
      661 tttttacgct cttgggcagc ccggcttggt cagcccaagc agccgatgcc gataaaaaac
      721 cggacgataa agtaactgaa actaaagaaa gcaaaaaagg cagcaaatca ccggtgagac
      781 tgcaatcggt atcggtaaaa gctaagaaaa acactaaaac cctggaaacc aaacacgata
      841 cgcccacggc tatatcggtg gtaaccggcg aaacactgaa tcgggaaatt gctcaagatt
      901 accaggcgat ttctaagcga ttatccaatg tcacctttaa ccagaataat tcgcggggcg
      961 cgtctttatc tattcgcggc ataggtaaac ggggatttaa cgaagtgcaa gatcccagtg
     1021 ttttagtgat acaagatggc gcaagtttcg gcctgacggc cttaggaaat tttgactttt
     1081 acgatataga agcggtggag agttaccggg gccccactgg caccctaggc ggtaaaggcg
     1141 gcagtgctgg gcaagtgaat gtcattacta agcagccgag ttttaaagcc agttctgatt
     1201 tttccatcgc ctatggctct tgggataccc ttgtggtgcg cgccgccagt ggtgggccga
     1261 ttattgatga tttgctcgcc tggcgcggag cgctgattgt tgataaaggc gagggatatt
     1321 ttcgcaatac ttttggttat gacaccaata atactcaata caataaaaac cgagctgcgg
     1381 ggcgcataca attattgtta acccccagcg aagagttgag cgtgcgggtt agttacgacc
     1441 ggcagcccaa ggcaccccag ctggaaaatg gcctcaccat acgcaaggac cagccagaat
     1501 attatgaagg cttttctttt gccgattatg ttgcgggcaa ctataccggt gttcaaccca
     1561 gcccaaccga tcccaatggc accacaccgc gggcaaaatt gtacggctat aagtcactag
     1621 taagcgctga ccctacgaac ccaaatagta caattaaaaa agagcaatgg aatccaggtc
     1681 ggccttggtt caccaatcgg ccaagccctg aaaatccggg ccacaattat gactacaatg
     1741 ccgaatatgt aggcacagac gacaatccca atattcattt taatgagcaa cagggccagt
     1801 atttatcaac cggcggctgg tcgggggaag tcagttggtt gcgcgacgat tttaaactca
     1861 cttctattag cgcctggcgg tctttttatt ttgatgccca caatgatgaa ggcacgtcgt
     1921 ttgatatcag taaacacggc ggcggcagtg tcgatttcca tcagtacagc caagaatttc
     1981 gtatcgcctc aactcctggc cgcacactgg attacaccgc tggtattttt ttgtataagt
     2041 ctgacactga tgtacaaagc cgtaccagtt ggggcagcga tgccggtgct tggtttgcga
     2101 ataccggcca atacaatacc ttggatcgca atgctggtgt taaccgcggc gccggtttag
     2161 cgctgctaaa agactcactg gaggatgtat ttagaaaagg cgatatttgg gttgaaacca
     2221 aaagccaagc cgtatttggc caaatagatt ggcactggtc tgaggcggcg actttaactg
     2281 caggtttgcg cctaggccgc gaggatcgca ccaccgaaga tgccctagta ctggtaaatt
     2341 acggcgcagc aaactggtta aacccggtga aaatcaatac gtttaattta ggcgggtttg
     2401 cttctacaac aaccgctgtt gccgccagtg gcaatgcgcc cgccatcccc gtgggctcac
     2461 tgggcaggct agaataccag ctcgacccag ccaccaataa aaaaatccaa gttttaatca
     2521 acgacaacac agccgcgcaa ttacagttag cggatcaagc agccaatcgc tattatggtg
     2581 ctgcaattaa cgccaacacg cccggctctg cctacaatag cctcaccgcc gcgcaaaaga
     2641 gccaaattgc agccgccaaa gcactgcgca acacccaaat tggccagctc attcccaacg
     2701 taaaaagtag ttacgaagat aatttagtca cctccaattt aagccagcgc tataaattta
     2761 atgagcaaat caccggttat gtcacctggc aacacgggga aaaacccggc accgctttta
     2821 atattaccgg tgtgccttca ccggtaaaag ctgaaaccac caatgcattt gagctggggg
     2881 taaaaacctt tttattcaat gaaaccctaa cactgaacgc cgatattttt cgcttaaata
     2941 ttgacgatta tcagcaggcg attcgcaccg tggatacctt tgccacccaa agcgccctag
     3001 acaatttaga tcctaatctt accggtgatg cattaaaaaa tgcccagaat gccgccactg
     3061 tttatatcac ctctcagggc aatgtgccta aggttacggt acaaggcttg gaagtggatg
     3121 gcgcctatac aggtgtacct catactacaa tccgttttag cggcgcctat aacgatgccc
     3181 gctacgggga atttactaac tcacccaagc cggatgaact ggcctattta aaagagccat
     3241 ttattgataa atccaacgag cttttgcccg gcgcggccct ggttactttt aatgtgggtg
     3301 cagaataccg cattccattt ttgcaggcgg aatttcacac gagttttaat acctcctaca
     3361 ctagcgattt taatagcggc gatacatttt caaaatacgc ggatatagat ggctattcga
     3421 ttaccgatac atccctcggt tttgccagta aggacggtaa atacgacgtt agcctagtgg
     3481 tgaaaaatgt gttggatgaa catcctcacg aagttgcatg gaccacctac acaccctata
     3541 tacacagacg ttgggccggt ctcgtgttcg actcccattt ttaactctgc cacagggggc
     3601 ctaagccccc ttcttcgccg gatcaataat tacttgccgt aacagccgca ggaatctgca
     3661 caaagtgctc agaatcaaac actccgaaaa ttagctgtgc gatttaatgc agaaaaaggc
     3721 tatttattgc ccgcatcagc gaatttttaa acataatttc aaccactcta ctggaacaag
     3781 tgttgctata cctgtttttt gcagatgcat cgcctgactc tgcgcaaagg ttgcagccca
     3841 ccggacattt tataactctc tcaatatttt ataactcact taaacaggta gaccatgaac
     3901 aaacccatat cctttttcca tgccctggct tttaccgccc tagccctacc acttgccgta
     3961 caggcggcga gcctgcaaca ggccgatgca acacttaaag ctaaagccac aacttcacta
     4021 acctttaagg gcacgggcgc ctggtatcaa tttggccaag caccggcacc tactttagcc
     4081 tggcccaaat ttgacgtcag cagttatcaa gctgatatta attttgagaa aactgctggc
     4141 cgagtgcaaa ttacgcgcaa acaatccttc gagcccggcc gcaatcgccc tgcgcccact
     4201 gagcaaaaag cggatcaata catcagcgat accatcgcct ggaacctcgc cacaacacca
     4261 cctgcgaata atgcaccggc tgccacggca caacctgccg cactcgaaga gcgtaaagcg
     4321 gaaattattt ccactccaca gggttttatt cgcgccgccc tcgccaataa tgccaccagc
     4381 aaacccacca aaaatggcat tgaagtaaat ttcacgctgg ataaaaaata tcaatataaa
     4441 ggcattatta acgataaaaa cctggtggag agcgtgcaaa cctggatcga caatcctgtg
     4501 ctcggtgaca cactgtacga aaccaccttt gcagattatc aagagcgctc aggtgtgcaa
     4561 tttccggcgc gcatcagccg taaactgggc ggccatccta ttttagatat tagcgttagc
     4621 gaggtgcagc caaatggcaa tgtcagtatt gcagtgcctg ccgaagttac tgcagcaccg
     4681 cctgcaatta cggttacctc aaccaagttg gccgaagggg tttattattt aaccggcggc
     4741 acacaccaca gcgttgctat cgaacaagca gatcatgtgg tattagtgga agcgccacag
     4801 catgaagcgc gctcattggc ggttattgcc aaggttaaag aaatcctacc caccaaaccc
     4861 attaaatacc taattaatac ccatgcacat tttgatcacc taggcggtgt gcgcaccttt
     4921 gttgatgccg gagccactat agtcacccac aaggataacc aagcctatta tcaaaaaatt
     4981 tggcagcaac cacacagcct taaccccgac aaattagccc aatcgaagaa aacacccaag
     5041 tttgctacct ttagcgctaa acacaccctg ggtgatagca cgcgtaaaat cgagctatat
     5101 cctctgacag gcaatagcca caacgatgcc tttgccttta tctatttacc caatgaaaaa
     5161 atcctcatcg aggcggatgc ttacacaccg ctagcccaag gtgtgccggt acccgctaac
     5221 gttaatccct attcacaaaa cctctatgag ttaattcaaa gccagaaact ggacgtagca
     5281 caaattgcag cgctgcacgg cccaggtgtg gttactctgg cggatttaaa aacctatata
     5341 ggcttaaaat cctaggctaa gtattacact ctaatcctgc gcagggatgt gcagattttt
     5401 agcattttaa gctctgcatt tagcaacatg ttgaagaccc acacccaggc ttttgaactg
     5461 tgccgttagc tttaatcagc gcgaaatttt tgatcccaaa cctattttat ctgcatttag
     5521 ccatcttttg tccgcactga gccctccatg atatccatta aattatcgcc acttacattc
     5581 gttttttgcc ttggcctaag cactttatgt gcagcaagcc ccagcattt
//
LOCUS       DAOQGS010000250         5618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7264_length_5618_cov_5.048175, whole genome shotgun sequence.
ACCESSION   DAOQGS010000250 DAOQGS010000000
VERSION     DAOQGS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7264_length_5618_cov_5.048175"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PKZ52_10260"
     CDS             complement(<1..1475)
                     /locus_tag="PKZ52_10260"
                     /inference="COORDINATES: protein motif:HMM:NF028151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HNG60197.1"
                     /translation="MLKFLRVALAAALLSGCSGDPANSSSSSSGSSSSSAGKTSSSSS
                     SGKTSSSSSTSSTSSSSSSSSSSSSSGRASSSSSSSSSTSSGGGDAARGLSLYNAQCK
                     NCHTGSSAVGVMRYSAISQLAGVIDSTMPKGNPDACNTQCAQDISAYLWTLRTSSSSS
                     SSSSSSSSSSSNSSSGNFVAGQVCGDRLIYGARQLKLLTRDEYQKSVQDLLGVTDDVR
                     GDIPADAKVHGFFNNLQSSVTETHADAYLFVAEKVANFAASQNFKTLPSCADLTEAAC
                     GAKFADEFAPKVFRRPLTAIERAAVVKIFDAKYSGGDIKVAQSLGIQALLTAPGFLYR
                     SELGEPVMRHAADGNQYVAGTPVETTAGPNFSGKVYGEAQTDGSWMIRMRDGQTPYIE
                     QHMKFYPAGTLIQVTLRGQAAGDVTPKLELRTGGTTQITEDINFSEYRTLKYYVSKLD
                     NTQALRFELKNGGAVAGVDRVVYIKSVSVSPATLMPGAADK"
     gene            complement(1946..4576)
                     /locus_tag="PKZ52_10265"
     CDS             complement(1946..4576)
                     /locus_tag="PKZ52_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HNG60198.1"
                     /translation="MKKLLWSILVLSLAACGSKGGGGQSSPSGSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGSPLPFITLLESYAVDENSPFKLPIT
                     VTGVGPFKYQLVYSPDVALFKLDTVTGVITSKTPFDFETPQSAEGTNIYSISIVVTDS
                     KKQSVTKDISISVNNVDEHELVVDFPINGANMGAAANKVHVRGHITESGKTLMQIPSH
                     LSVVVNGVLASIDPARPSMWLAEVPIVEPVNDISVQLKNGVEIEQEKSLKLANNPLMG
                     ERIADGVHDYYIGADLSRKKIYKRSFDDANNYSLILSSSKEGYEDCDQFGGFSLSKTG
                     KYFSAICTASARELGSIVYCNLDSGSCKKGGLAYAGYPARVNWADDRHLLYAISDTEI
                     GLLDTLAGVSKKLVFETGYSFKYSTPIDVDQGQVNLFLEKRENNIGGPDYYSFPLKTH
                     IDNPELEQTVQFTSISARPTSSWNFFSFNGHFYSPTTNGLYVTSQTDGSTSFLKIDKF
                     RTDTQYSSNLVHAANGLLVFDQNGALFSYDIANNKTKDLDSKKYFVGRFETDLSPDSK
                     RLVLFDAIDRRFAFYDLEAGAALNYDAVDQPASENGNTFGSTAVDWQNKLIYRALTGE
                     WGGVVPSPLTPLIVSYDAVTKAYKTILTSAQLAVQMNRSFERLSPRRIAFKAATQELV
                     IHLLLFSGSGSEQLICSLNVKTREVKTLAVFPLANQTGSVDFAEIFMSRLNPALNGIA
                     FAHWGYLDDKGGGVEWFNFDGARTDLLPPQQPYYLTTRGVMSPVGDRYYGIGFKRLAG
                     PSSTNTSNGELFYIDMATKNRTVLASESQGLGLTPPWLEPVYDENRDLLIDFAAPNLW
                     VFDTVTGDRVVKPVELPASN"
     gene            5063..>5618
                     /locus_tag="PKZ52_10270"
     CDS             5063..>5618
                     /locus_tag="PKZ52_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNG60199.1"
                     /translation="MKLLHLYLFPITIGFALQASHASAHAEGTTLSNFNHQTPAVNGP
                     FSRSVQSLKTYQTPQWFADAKFGIWAHWGPQAVPRAGDWYARNMYIPGTPQYEHHLKH
                     YGHPSESGFKDIIPLWKAEKFNPDALMQLYVKAGARYFVSMAVHHDNFDLWDSRYQRW
                     NAVKMGPMRDIVGEWQKAAHKYKLP"
BASE COUNT         1582 a         1352 c         1254 g         1430 t
ORIGIN      
        1 tctttatcgg cagcacccgg cattaaagtg gcgggcgata cggaaacgga tttgatatac
       61 accacacggt ctacgccagc cacggcgccg ccatttttta attcaaagcg cagcgcttgg
      121 gtattatcta atttgctcac ataatattta agcgtgcggt attcactaaa attaatatcc
      181 tcagtaattt gtgtggtgcc accggtgcgc agctctaatt ttggtgttac atcccccgcc
      241 gcctgccccc gcaaagtcac ctgaattaag gtgcctgccg ggtaaaactt catatgctgc
      301 tcaatataag gcgtttggcc atcgcgcatg cggatcatcc aactgccgtc ggtttgtgcc
      361 tcaccataaa ccttgccgga gaaattcggc ccggctgttg tttccaccgg tgtaccggca
      421 acatactgat tgccgtcggc ggcatggcgc atcactggct caccgagttc agagcggtat
      481 aaaaagccag gtgcagttaa taaggcttga atacccaacg actgagcaac cttaatatcg
      541 ccaccggaat attttgcatc aaaaattttg accactgctg cgcgctcaat ggcggtgagt
      601 ggacggcgga ataccttagg cgcaaattca tcggcgaatt tagcaccgca ggcggcttca
      661 gttaaatctg cgcaactggg cagggtttta aaattttggc tagcggcaaa atttgctacc
      721 ttttctgcta caaataaata ggcgtcggcg tgggtttccg tcaccgacga ctgtaaatta
      781 ttaaaaaaac catgcacctt ggcatcggca ggaatatccc cgcgcacatc atcagtcaca
      841 cccaataaat cctgtacaga tttttgatat tcatcgcggg tgagcaattt taattggcgc
      901 gccccataaa taaggcgatc accacacacc tgcccggcca caaaattgcc actggatgaa
      961 ttgcttgagc tgctggacga acttgatgag gaagacgagc tagaactgct cgtgcgcaag
     1021 gtccataaat aagcgctgat atcctgggca cactgagtat tacacgcatc tgggttgccc
     1081 ttaggcatgg tgctatcaat caccccagcc aattggctta tcgccgaata acgcatcacg
     1141 cccacagcgc tggagcccgt atggcaattt ttgcactggg cgttatacag gcttaaaccc
     1201 cgcgccgcat cgccaccgcc ggagcttgtt gaactgctgg aactactcga actggacgct
     1261 cgacccgagg agctactgga gctggatgag ctgcttgaac tggaagtact actagttgag
     1321 ctggatgagc tggttttacc cgatgatgaa ctggagctgg tcttacccgc tgagctggaa
     1381 gacgagccag atgaagaact ggagctattg gcagggtcgc cagaacagcc cgacagcagt
     1441 gcagccgcca gggccacacg caaaaactta agcattattg attcactccg acttatagtg
     1501 caaaaaacta tcagatataa gatacaggac ggagcgagaa ataaaaggcc tttggggcgg
     1561 agattggggg gctttgctca ggcttgtgaa atcgtttgca ttgtcatgag cgctaaggca
     1621 gagcggccct ggcaatataa tcggcgggtg cctattttta ggacgaataa ataggaaatt
     1681 atgtggctag gcaattaatt tttacgagcc gccgaattaa tgccatgtgc caccgaattc
     1741 ttgcatttaa catagggagg aacacttgcg actaaagtca taagggcact cacccatccc
     1801 gttagcctaa tgtattaacc cagcggtatt taacgccggg ttaataagct ttcccccgaa
     1861 acaacagcaa aatacaggcg ttcacgcaaa attacagacc ctatagtgca gccgcacagc
     1921 atccttgcag tgccaataat atttatcaat tgctggcggg aagctcaaca ggttttacga
     1981 ctcgatcccc tgtaactgta tcgaaaaccc ataaattagg cgctgcaaaa tcaatcaaaa
     2041 gatcacggtt ttcatcgtat accggttcaa gccaaggagg cgtaagccct aagccttgcg
     2101 actccgaggc tagcactgtt ctatttttag tggccatgtc gatataaaac aattctccgt
     2161 tactcgtatt ggtagagctt gggcctgcga ggcgtttaaa tccaataccg taataacgat
     2221 cacccactgg gctcattact ccccgcgtag ttaaataata gggttgttga ggaggcaata
     2281 aatcggtacg agcgccatca aaattaaacc actcaacccc gccaccttta tcatctaaat
     2341 aaccccagtg agcgaatgcg atgccattta acgctggatt aagccgcgac ataaatatct
     2401 ccgcaaaatc tacactaccg gtctgattcg ccaatggaaa tacagccaag gttttgactt
     2461 cgcgtgtttt aacatttaac gagcatatca actgctctga gccagagcca ctaaacaaaa
     2521 ggaggtgaat tactaattcc tgggtggccg ccttaaaggc tattcgtcga gggctcagcc
     2581 gctcgaatga gcgattcatc tgtaccgcca actgagctga cgttaagatc gttttataag
     2641 ccttagtaac tgcgtcataa ctgacaataa ggggcgttag gggtgacggc acaacacccc
     2701 cccattcacc tgtcagcgcg cggtatatca atttattttg ccaatcaaca gcagtgctgc
     2761 cgaatgtgtt gccattttca cttgctggct gatccaccgc gtcataattg agcgctgctc
     2821 cagcctctag atcataaaat gcaaaccgtc ggtctatcgc gtcaaataat accaagcgct
     2881 tggaatctgg gcttaagtca gtctcaaaac gaccaacgaa atattttttt gagtcaagat
     2941 cttttgtctt attgttcgca atatcgtagg aaaataacgc accattttgg tcaaatacta
     3001 atagcccatt tgcggcatgg actaagttag atgaatactg ggtatcagtt ctaaacttat
     3061 caatctttaa aaaagaagtt gagccatccg tttggcttgt gacatatagc ccgtttgtag
     3121 tcggtgagta aaaatgacca ttgaagctga aaaaattcca acttgaggta gggcgggcag
     3181 aaatcgaagt aaactgaacg gtttgttcca gttcaggatt atctatatga gtcttgagtg
     3241 gaaaggaata ataatctggg ccgccaatat tattctctct tttttccaga aaaaggttga
     3301 cctggccttg atcaacatca ataggagttg aatatttaaa gctataccct gtttcaaata
     3361 ctaatttttt tgacacccca gcaagcgtat ctaacaaccc gatttcagta tccgagatag
     3421 cgtatagtag atgtctgtcg tcagcccagt taacgcgcgc agggtaacca gcataggcta
     3481 gcccgccttt tttacaggag ccgctatcta aattacaata aacaattgaa cccaattccc
     3541 gtgctgaagc ggtgcaaatt gcagaaaaat atttaccagt cttacttaat gaaaatccgc
     3601 caaattgatc gcagtcttca taaccctctt tagaagaaga aaggatcaga gaataattat
     3661 tcgcatcatc gaatgaccgt ttatagattt ttttcctgga caagtccgca ccaatgtaat
     3721 aatcgtgcac accatcagcg attcgttcac ccataagagg attattggca agcttcaagg
     3781 atttttcttg ttcaatttca acaccatttt ttagctgaac agaaatatca ttgacgggtt
     3841 caacgatagg tacttccgca agccacatag atggacgtgc agggtcaatg ctagccaata
     3901 cgccattaac taccactgag agatgagaag gaatttgcat gagcgtcttc ccgctctcag
     3961 taatatgccc tcgtacgtgc actttatttg cagccgcccc catattcgca ccattaatgg
     4021 ggaagtccac caccagttca tgttcatcta cgttatttac actgatgcta atgtcttttg
     4081 tcacagactg ctttttacta tccgttacca ctatagaaat agaatagata ttcgtccctt
     4141 cggcactttg aggtgtttca aaatcaaatg gggttttact ggtaattacc cctgttacag
     4201 tatcgagttt gaataacgca acatctggcg aataaaccaa ttggtactta aacggcccta
     4261 cgcccgtaac ggtaatgggc aatttaaatg ggctattctc atcgacggcg taactttcca
     4321 acagggttat aaatggtaat gggctgccgc ttgaagagct actggatgag ctggaagacg
     4381 aactagatga ggaacttgaa gaactagatg atgaactgga tgagctcgat gatgaactgg
     4441 atgagctcga cgatgaactg gatgagctcg aagacgaact cgaggaagaa ccagagggac
     4501 tgctttgccc tccaccacct ttactgccgc aggcggcaag cgataacact aaaattgacc
     4561 acagtaattt cttcatacga cactccgttt aacaacaatc aaaaaaactt cccgctataa
     4621 cgcggcatga tcggctttga ggggccaaac cgcaccagcc ctattccagc tcctatggct
     4681 tgaagagact aatttagctg atcaaaaaaa acggcgcatt atagtcgaat gagccctgac
     4741 aggccacatc agccgagaat ctttagactg ttttgcaccc acttaagagg tcgatcacag
     4801 aaggggacga attaaatctg tttaagcgtc aaggctattt ctaagcgcgc ccaaggatgc
     4861 tccaattcag gcctaatgca ggataatctt gaaaacgcac cgagctatgc ccccttcggt
     4921 catcagcggg cttacctccc ttgttcggga cagctgcaac ctatcccctc cggcagcctt
     4981 aatcagaagc tgcttaaatc atttagcgtt taattaatat agtgtgatag aacaccgaac
     5041 aataataatt aggcatacga gcatgaaatt gttacattta tatttattcc caataaccat
     5101 aggctttgcc ctgcaggcat cgcatgcatc tgcgcacgcc gaaggcacta cactgagtaa
     5161 tttcaatcac caaaccccgg ccgttaacgg gccattctcc cgctcagttc aatcacttaa
     5221 aacctaccaa acaccccaat ggtttgccga tgctaaattc ggtatttggg cccattgggg
     5281 gccccaggcg gtgcccaggg cgggcgattg gtatgcgcgt aatatgtata ttccaggcac
     5341 gcctcagtat gaacaccatt taaaacatta cggtcatccg tctgaatctg gctttaaaga
     5401 tattatcccc ctgtggaaag ctgagaaatt caaccctgat gctttgatgc aactctatgt
     5461 aaaagccgga gcgcggtatt ttgtttctat ggccgtgcat cacgataatt ttgatttgtg
     5521 ggattcccgc tatcagcgct ggaatgcggt aaaaatgggg ccgatgcggg atatagtggg
     5581 tgaatggcaa aaagctgcgc ataaatataa attgccat
//
LOCUS       DAOQGS010000251         5606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7287_length_5606_cov_4.250045, whole genome shotgun sequence.
ACCESSION   DAOQGS010000251 DAOQGS010000000
VERSION     DAOQGS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7287_length_5606_cov_4.250045"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /gene="rplM"
                     /locus_tag="PKZ52_10275"
     CDS             <1..329
                     /gene="rplM"
                     /locus_tag="PKZ52_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNG60200.1"
                     /translation="RLRGKHKPEFTPHVDTGDYIVVINAEKVVVTGKKAKDKMYHHNT
                     GYPGGLRTISFEKLIDKAPERTIQSAVKGMLPKGPLGRAVFKKLKVYAGASHPHTAQQ
                     PKELNL"
     gene            342..734
                     /gene="rpsI"
                     /locus_tag="PKZ52_10280"
     CDS             342..734
                     /gene="rpsI"
                     /locus_tag="PKZ52_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNG60201.1"
                     /translation="MAGEQYYGTGRRKTSTARVFLSLGSGALSINGRTLENYFGREVA
                     RMIVRQPLELIGAGDRFDINITVKGGGSFGQAGAIRHGLTRALMAYDETLRPTLRAAG
                     YVTRDAREVERKKVGLRKARKRPQYSKR"
     gene            1029..1631
                     /gene="petA"
                     /locus_tag="PKZ52_10285"
     CDS             1029..1631
                     /gene="petA"
                     /locus_tag="PKZ52_10285"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNG60202.1"
                     /translation="MSNDEINRGRRRVLTAVTSAVGAVGAVGAAVPFVGSWSPSAKAK
                     AAGAPVEINISKIEPGQMVTVEWRGKPVYVVRRTKEALEGLKVNEAQLRDPTSAKSVQ
                     PSYAKSESRAISGKEEIVVLLGVCTHLGCAPGFEPKVGAVDLSGGKTWQGGFFCKCHG
                     SMYDLAGRVFQGVPAPLNLEVPAHKYLSDSVILIGEDQGA"
     gene            1632..3683
                     /locus_tag="PKZ52_10290"
     CDS             1632..3683
                     /locus_tag="PKZ52_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNG60203.1"
                     /translation="MMNWLTGLWQWVDKRLPVQRAWDTHMGKYYAPKNFNFWYFFGVL
                     SLLVLVNQLLTGVWLTMNYEPTAQGAFASVEYIMRDVPYGDVLRYMHSTGASAFFIVV
                     YLHMFRGMMYGSYKAPRELVWIFGMFIYVLLMAEAFMGYVLPWGQMSYWGAQVIVNLF
                     GAIPVVGEDLVQWIRGDYLISGATLNRFFALHVVALPIVLLALVVMHILALHEVGSNN
                     PDGIEIKKKKDANGIPLDGVPFHPYYTVHDLVGITVFLFAFCGIIFFMPEMGGFFLEH
                     ANFEPANGLKTPDHIAPVWYFTPFYAMLRAVTIEIGPLNSKFLGFVVMAAAIAILFVL
                     PWLDKSPVKSIRFKGLFSRVALIVFAASFVILGYLGVKPANEARTALAQICTVVYFLY
                     FIGMPFWTKREKTLPVPDRVQAKGLGKHWVLGGLLLFIVLVVAPIKAVANEQLGHCGT
                     VACDHFEPNFDDKASLQRGAKYFVNYCMGCHSANFSRFSRVAEDLEIPEKLVAENLIF
                     GDQKIGETMGIAMAPKKSKAWFGATPPDLSLETRARSPEWVFTYLRSFYRDDTRPTGV
                     NNKVFPNVGMPHVLIELQGLPECATGPHRDIHGKTERDKLGEPKMIECGKLEVGKVKG
                     SMTPAEYTEAVEDLVNFMSYLAEPGAAKREHIGWYVLAFLSVFFICVLLLNREYWKDV
                     H"
     gene            3947..4348
                     /locus_tag="PKZ52_10295"
     CDS             3947..4348
                     /locus_tag="PKZ52_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNG60204.1"
                     /translation="MSMTSSKPYLIRALYEWILDNNLTPYLAVDALSPSVQVPQQYVN
                     KDGQIILNIAPRAVDAINLSNEAIVFSARFGGVSTDIYVPCFAVLGIYARENGKGMMF
                     EREAEPPPSEPVPPKSSGSSPDKKPSLRVVK"
     gene            4425..4913
                     /locus_tag="PKZ52_10300"
     CDS             4425..4913
                     /locus_tag="PKZ52_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60205.1"
                     /translation="MIPIIIDIEASGFGSGSYPIEIGFIDDNGQRYCSLIKPLPEWTA
                     WDEGAFNMHGISRAYLASHGKPAALVADELNQLLAGKRVFSDGWVADQFWLNTLFQAL
                     GKWPSFTLSPIEGIQTPRQHECWDLTLRKLREQHNEQRHRASSDAYLIQLAFITSAKI
                     PS"
     gene            5117..>5606
                     /locus_tag="PKZ52_10305"
     CDS             5117..>5606
                     /locus_tag="PKZ52_10305"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013743.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNG60206.1"
                     /translation="MTESVVVLGIARTAMGGMQGVFGELPAPQLGGAAIKGALEDANI
                     SPAEVTEVLMGCVLPAGIGQAPARQASLAAGIAKSVPTSTVNKVCGSGMKTVMMARDS
                     LLAGSNKVVVAGGMENMSLAPYMLTKARSGYRLGHGELLDHMFLDGLQDAYQGGLMGN
                     FAE"
BASE COUNT         1266 a         1360 c         1460 g         1520 t
ORIGIN      
        1 gccgtttacg cggtaagcac aagcctgaat ttacccccca cgtggatacc ggcgattaca
       61 ttgttgtaat caatgccgag aaagtcgtgg ttaccggtaa aaaagcgaaa gacaagatgt
      121 atcaccacaa caccggttac ccaggcggct tacgcactat cagctttgaa aagctgatcg
      181 acaaagctcc tgagcgcaca attcaaagcg ctgttaaggg tatgctgccc aaaggcccat
      241 tgggtcgtgc agtattcaaa aaattaaagg tctatgccgg tgcgtctcat ccgcacacag
      301 cgcaacagcc caaagaactc aacctataac ggaggctgca aatggcaggt gaacaatatt
      361 acggtactgg tcgtcgcaaa acttctaccg cgcgtgtatt tctttcgctg ggtagcggtg
      421 cgctgagcat taatggccgc actttggaaa actattttgg ccgtgaagtg gcgcgcatga
      481 ttgtgcgtca accactggag ctgatcggtg cgggtgaccg cttcgacatc aacatcactg
      541 ttaaaggtgg tggtagcttc ggtcaagcgg gcgctattcg ccacggtttg acgcgcgccc
      601 tgatggccta cgacgaaacc ctgcgtccca ccctgcgtgc tgcaggttat gtaacgcgtg
      661 atgcgcgtga agtggaacgt aaaaaagtcg gtttgcgcaa agcgcgcaag cgtcctcaat
      721 actccaaacg ttaatcctat cgtttggttg gagcccgaac tcagttcggg cttttttttg
      781 cgtgtgcgtt ttacctaagc ccccagttgc cttgtaagca caggggtttt tcattaccat
      841 tgcgcgcttt tcgcggtgcc ggttgattcg tgtttgaatg atttaaatca gttgctggct
      901 aggttcaaag ggtagtattg cggttttttg atcggcggta attacccttg cactcaattc
      961 gaagttaata atttgtccta tcattctttt ttcccaattt tttccttttg ccacaggaga
     1021 tatacgtcat gagtaatgac gaaataaacc gcgggcgtcg ccgtgtttta acggcagtga
     1081 catctgccgt gggtgctgta ggcgcggtcg gtgcagccgt tccgtttgtc ggctcctggt
     1141 ccccaagcgc taaagcaaag gcggccggtg cacccgttga aatcaatatc agcaaaattg
     1201 aacccggtca gatggtgacg gttgagtggc gcggaaagcc agtttatgtg gttcgtcgta
     1261 ctaaggaagc tctagaagga ttaaaggtca atgaagcgca attgcgcgac cccacttcag
     1321 ctaaatcggt gcaaccctcc tacgccaaat cagaaagtcg cgctatcagc ggcaaagaag
     1381 aaattgtggt gctgttaggt gtgtgcaccc acttaggctg cgcacccgga tttgagccca
     1441 aagttggcgc tgtggatctt agcggtggca aaacttggca gggtggtttc ttctgtaaat
     1501 gccacggttc tatgtatgac ttggctggtc gcgtatttca gggcgtacca gctccattaa
     1561 atttagaggt gcccgctcac aagtatctta gtgattcggt cattcttatc ggcgaagatc
     1621 aaggggccta aatgatgaat tggctaacgg ggctatggca gtgggtagat aaacgtctac
     1681 cggttcaacg cgcttgggac acccatatgg gtaagtacta cgcgcctaag aattttaact
     1741 tttggtattt tttcggtgtt ttatcgttgt tggtgctggt caaccagttg ctaaccggtg
     1801 tttggctgac catgaattac gagccaaccg ctcagggtgc ttttgcatcc gttgaatata
     1861 ttatgcgcga tgtgccctac ggcgacgtac tgcgctatat gcactccacc ggtgcttctg
     1921 ccttttttat cgttgtttac ctacatatgt tccgcggcat gatgtacgga tcctataaag
     1981 ctccccgcga gttggtgtgg atcttcggta tgttcatcta tgtgctcttg atggccgagg
     2041 ccttcatggg ctatgtattg ccttgggggc aaatgtccta ttggggcgcc caggtgatcg
     2101 tgaatttgtt tggtgctatt cctgtggtgg gtgaagacct ggtgcagtgg attcgcggcg
     2161 actatttaat ttctggtgcc acactgaacc gcttttttgc gctgcacgtg gttgcgctgc
     2221 cgattgtgtt gttggcttta gttgtcatgc atattctcgc cttgcacgaa gtgggctcga
     2281 ataatcccga cggcattgag attaaaaaga aaaaagacgc caatggtatt cctctggacg
     2341 gtgtaccgtt ccacccttat tacaccgtcc acgacctggt gggtatcact gtatttctgt
     2401 tcgccttctg cggcattatt ttctttatgc cggaaatggg cggctttttc cttgagcacg
     2461 cgaacttcga gcctgccaac ggcttaaaaa ctcctgatca cattgcgcct gtttggtatt
     2521 tcacgccttt ctacgccatg ttgcgtgcgg tgacgattga aatcgggccg ttaaattcta
     2581 agtttttggg ttttgttgtg atggccgcgg ccatcgccat attgtttgta ctgccttggt
     2641 tggataaaag cccagttaaa tccattcgtt ttaaaggctt gttcagccgc gttgcgctga
     2701 tcgtgttcgc tgcatccttt gttattttgg gttacctggg tgttaaacca gctaacgaag
     2761 cgcgtacggc attggcacaa atctgcacag ttgtatattt cctctatttt attggcatgc
     2821 cgttttggac caagcgtgaa aaaaccttgc cagtgcccga ccgcgtgcag gcaaaaggcc
     2881 tgggcaaaca ctgggtgttg ggtggtttgt tgctgttcat cgtcttagtg gtggcgccca
     2941 ttaaagcggt tgccaatgag caactggggc actgcggtac tgtggcctgc gaccattttg
     3001 aacccaactt tgacgataaa gcctccttgc agcgcggtgc aaaatacttc gttaactatt
     3061 gcatgggctg tcactcggcg aatttttcgc gcttttcccg cgttgcagag gatttggaaa
     3121 tccccgaaaa actggtggcc gagaatttaa tctttggcga tcaaaaaatt ggtgaaacca
     3181 tggggattgc catggcgccg aaaaaatcta aagcctggtt cggcgccacg ccgccggact
     3241 taagccttga gacccgcgcc cgcagcccgg agtgggtatt tacctatctg cgcagctttt
     3301 acagggacga tactcgccct accggtgtta acaacaaggt tttcccgaac gtaggtatgc
     3361 cccatgttct tatcgagttg cagggcctgc ccgaatgcgc caccgggccc caccgcgata
     3421 ttcacggcaa aaccgagcgc gataaacttg gcgagcctaa gatgattgag tgtggcaagt
     3481 tagaagtggg caaggttaag ggctccatga caccggctga atacaccgaa gccgtcgaag
     3541 atctggtgaa ctttatgagt tacctggccg agccgggcgc tgccaagcgc gaacatatcg
     3601 gctggtatgt gttagcgttt ctttctgtgt ttttcatctg tgtgttgcta ctcaaccgcg
     3661 agtattggaa agacgtgcac taagtggtat aggccggggg tgttcccccg gccttgtttt
     3721 ttggctaata acaataaacc tatagctctt tggcttgata gcaggctgtt taggctcgat
     3781 ttgaatacac cgagctaaat cagttgagcc ttgttaactc tcaccgctga cacaactgca
     3841 aggttgcgct aggctgagtg gtttcactag actttgttct ttccatcatg cggcacccag
     3901 gccttacttg tgtgcgctta aatagttgaa gcaggtgttt acgtttatgt ccatgacctc
     3961 tagtaagcct tacctgatcc gggctctgta cgaatggatt ttggataata acctgacccc
     4021 ctatttagcc gtcgatgccc tcagcccaag cgttcaagtg ccccagcagt atgtcaataa
     4081 agatgggcaa attattctta atattgcccc gcgcgctgta gacgcaatca acctctccaa
     4141 tgaagccatt gtcttttccg cccgttttgg cggtgtttca acggacattt atgttccttg
     4201 ttttgcagtg ttaggtattt atgcgcggga aaacggcaag ggtatgatgt ttgagcgcga
     4261 ggcagagccg ccaccgtctg aacctgtacc acctaaatcc tctggttctt cgcccgacaa
     4321 gaagccgagt ttacgcgttg taaaatagac ttacggctac cccagtctgg ggcatacacc
     4381 taatcggcgt taggaaatta agaccctatg gatgcaccca ttttgtgatt cccattatca
     4441 tcgacattga ggcatctggc tttggcagtg gcagttatcc cattgaaatt ggttttatcg
     4501 acgacaatgg ccagcgctac tgcagcctaa ttaaacccct gccagagtgg acggcctggg
     4561 atgaaggtgc atttaacatg cacggcatca gccgcgccta tctggcaagc catggtaaac
     4621 ccgccgcttt ggttgccgat gaactcaatc agttgctagc tggcaagcga gttttttccg
     4681 atggatgggt agccgatcaa ttctggttga acactctgtt tcaagcgctg ggcaagtggc
     4741 cgagttttac cctctcgcca attgagggca ttcagacacc caggcaacat gagtgctggg
     4801 atctgacgct gagaaaactg cgtgaacaac acaacgagca gcggcacagg gcatcaagcg
     4861 atgcttattt aattcagctt gcctttatta cctccgcaaa aatccccagt tgagtcaaac
     4921 ttctacttgc cataattatc cataccgcac accatgtgcg ttagttaaca ttgtgggttt
     4981 gttttggctt aagaaccctt aaaacaatcc gcgtgcgcac ctgaagttgc aattctatgc
     5041 gcatgcagag ttatccattc cacagatcgt agattcatta ccatccattg attgaacctg
     5101 aaagaggaaa accttcatga ctgaatcagt tgttgtactc ggtattgcta gaaccgccat
     5161 gggtggtatg cagggcgtat ttggtgaact gccagcaccc cagctcggtg gtgcagcaat
     5221 caaaggtgcc cttgaagatg ccaatatttc ccccgcagaa gtcaccgaag tgctgatggg
     5281 ctgtgtttta cccgcaggta ttggccaagc gccagcgcgc caggcgagtt tagctgccgg
     5341 catcgctaag tctgtcccta ccagcacagt aaacaaagtc tgtggctcgg gtatgaagac
     5401 cgtgatgatg gcgcgggatt ctctactggc tggcagcaat aaagtagtcg ttgcaggcgg
     5461 catggaaaat atgtcgctgg caccttacat gctcaccaaa gcgcgcagcg gttaccgctt
     5521 aggccacggt gaactgctgg accacatgtt ccttgatggt ttgcaggatg cctatcaagg
     5581 cggtctaatg ggtaactttg ccgaac
//
LOCUS       DAOQGS010000252         5571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7356_length_5571_cov_4.645395, whole genome shotgun sequence.
ACCESSION   DAOQGS010000252 DAOQGS010000000
VERSION     DAOQGS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7356_length_5571_cov_4.645395"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /gene="phaC"
                     /locus_tag="PKZ52_10310"
     CDS             <1..1809
                     /gene="phaC"
                     /locus_tag="PKZ52_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNG60207.1"
                     /translation="APSAATLRSRLMKQEEKLEILGYLTKFNHVFNDMIGEVTKRVLS
                     SQVKAEINPFSQTEKLADVLSSGVKLDTKLFLQQQFDFLQKQQLLWQNASRIFMGEML
                     EPLVQEEKSDKRFADGDWEGNPVFNYIKQAYLLNAEYVSQMVESLEFEDGKMGEQVRF
                     YTRQLVNSMAPSNYVFTNPEVCREILKTEGECLARGIDNFMRDLENSPSEAFKITQVD
                     INAFTLGETLAYTPGEVVFKNRLIELIRYFPAGSEVVATPLLIVPPFINKYYILDLDA
                     RKSLVKWLVERGYEVFMISWVNPDETYADTEFGDYMTEGVLAALDQVIDITGAEQVQA
                     AGYCVGGTLLGITQSYLTALNDKRISSCTFLTTLFDFSEPGEVGAYISGTLLPLLEQN
                     VTSKGYMDGRVLALSFSLLRENNLFWSFFIENYLKGKDPMPFDILYWNSDSTNLPAKA
                     YLYYLNNMYVEDRLKEPGALTIGGQPVDLSLIETPSYCLAARADHIVLWHAAYKSAKL
                     LGGAARFVLTESGHVAGVINPAATGKYPHWVAEHLPKQADQWFAQAQEKPGSWWLDWD
                     AWLRANCSATRAVAPVYAADNPSAPGDYVKRRLETI"
     gene            1900..2358
                     /gene="phaR"
                     /locus_tag="PKZ52_10315"
     CDS             1900..2358
                     /gene="phaR"
                     /locus_tag="PKZ52_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNG60208.1"
                     /translation="MITIKKYPNRRLYDTSKSQYINLEAIKLLVMTGQEFQVVDSKTD
                     ENLTKSILLQIITEQEGNEGQALLTQTVLKQLIRFYGSDLQGFMSQYLEQSVATFLDR
                     QEAMQSVMHDLLDKASPLNVFNQLMEQNMSVWRSFANPMGKSANEPKDKE"
     gene            2484..2801
                     /locus_tag="PKZ52_10320"
     CDS             2484..2801
                     /locus_tag="PKZ52_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNG60209.1"
                     /translation="MYEKFFEQAQTALKPFSELLTLNAKIAEEAAEKQKIFISELVND
                     SVAFAKTLSAQKDYSGIYQTQKSYLEGLQAKWVAASTDAYQTFTTTSESVGKVIKSAA
                     TVQ"
     gene            2992..3714
                     /locus_tag="PKZ52_10325"
     CDS             2992..3714
                     /locus_tag="PKZ52_10325"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNG60210.1"
                     /translation="MYQQFLDQAGTLFKPLQDLVSLNQTAFEALANKQSALVSEIIND
                     SISFTQKVLAEKDGNYWDLHKAYWEGVNQKLNQASQEAADYFTATQTKLSDLVQESLL
                     GSAMAEVTKHMPSVEAAVASALKPIETAKIKPAAEAAKVPAAKPAPAPALATKKPAAK
                     PAAKPAAKVADAKPAESKKPADAKKSVEAEKTVEVKSPVKPAEKPPAVKAPATEEKST
                     RVAGDDATVESVIRSIMAERNH"
     gene            complement(3933..4655)
                     /gene="phbB"
                     /locus_tag="PKZ52_10330"
     CDS             complement(3933..4655)
                     /gene="phbB"
                     /locus_tag="PKZ52_10330"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNG60211.1"
                     /translation="MGRLALVTGGTRGIGAAISLRLKKAGYNVVAAYAGNDEAAAAFK
                     AEHNIPVYKFDVADFDACAAAVEQIRRDFGPIEILVNNAGITRDSTLHKMSKEAWHAV
                     IDTNLSSCFNLCHAVIEQMRENNFGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGANKGITVNAIAPGYIATDMVRAVPEKVLEKIVAKIPVGRLGEADEIARGVEFL
                     VADESGFITGSTLSINGGQHMY"
     gene            4900..>5571
                     /gene="trxA"
                     /locus_tag="PKZ52_10335"
     CDS             4900..>5571
                     /gene="trxA"
                     /locus_tag="PKZ52_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNG60212.1"
                     /translation="MQRPVLIDFWADWCAPCKTLMPLLERLATEYAGAFLLAKLNADE
                     QRMIASQFGVQSLPTVILMKDGQPIDGFVGAKTEVEIRQFLDKHLPKAWDGQFLEAQA
                     LMAAGDFNGAVSLLRQAQQDSGGRADIVCALAECLIHLKRLDEAQSLLAQVRLVDQDG
                     RFHQVQALLELASNAQKAPEITDLEALLAADPASTALAHQLALQYSQHGYTSEALELL
                     YSLLKK"
BASE COUNT         1424 a         1329 c         1462 g         1356 t
ORIGIN      
        1 gccccctccg ctgccaccct tagaagcagg cttatgaaac aagaagaaaa actcgaaata
       61 ctcggctacc tcaccaagtt taatcacgtg tttaacgaca tgatcggcga ggtgaccaag
      121 cgtgttttat ccagccaggt aaaggcggaa attaacccgt tttcccaaac tgaaaaactc
      181 gccgatgtac tctcctccgg cgttaaatta gacaccaaat tattcctgca acaacaattt
      241 gattttttgc aaaaacaaca attgctgtgg caaaacgcta gccgcatttt tatgggcgaa
      301 atgctcgagc ccttggtgca ggaggaaaag tccgacaaac gcttcgccga tggcgattgg
      361 gaaggcaacc cggtatttaa ttacatcaag caggcttatt tgctcaatgc cgaatatgtc
      421 agccaaatgg tggaatccct ggagtttgaa gacggtaaaa tgggcgagca ggtgcgtttt
      481 tacactcgtc agttggttaa ctccatggcg ccgagtaatt atgtatttac caatccagaa
      541 gtctgccggg aaatattaaa aactgaaggt gagtgtttgg cccggggcat tgataatttc
      601 atgcgcgacc tggaaaacag ccccagtgaa gcctttaaaa ttacccaagt ggatattaac
      661 gcttttaccc tcggggaaac cctggcctac acgccaggtg aagtagtgtt taaaaaccgc
      721 ttaatcgaat taattcgata tttccccgct ggcagcgaag tggtggccac tcccttatta
      781 attgtgccgc cgtttattaa taagtattac atcctcgatt tggacgcgcg taaatccttg
      841 gttaaatggc tggtggagcg cggttacgaa gtctttatga tttcctgggt caacccggat
      901 gaaacctacg ccgatactga atttggcgac tatatgacgg agggagtttt agccgcccta
      961 gaccaagtaa ttgacattac cggcgccgag caggtgcagg cggcgggtta ctgtgtcggc
     1021 ggcactttat tgggcatcac ccaaagttat ttaacggcct taaatgataa acgcattagc
     1081 tcctgtacct ttttaaccac actgtttgat ttttcagaac cgggggaagt gggggcttat
     1141 atttcaggca ccttgctgcc tttgttggag cagaacgtga cgtctaaggg ctatatggat
     1201 ggccgcgtgt tggcattgag ttttagtttg ttacgggaaa acaatttatt ctggtcgttt
     1261 tttattgaga actacctcaa gggcaaagac ccaatgccct tcgatatttt gtattggaat
     1321 agcgactcaa ccaatcttcc tgccaaggcg tacctctatt accttaataa tatgtacgtt
     1381 gaggataggc ttaaagaacc cggtgcatta accatcggcg gccagccggt tgacctaagc
     1441 ctaattgaaa cccccagtta ttgtttggct gcgcgggctg accacatagt gctgtggcac
     1501 gcggcttata aaagcgccaa attattaggt ggcgcagcgc gttttgtgtt gactgaatcg
     1561 ggccatgtgg cgggtgttat caaccctgcc gccacgggaa aataccccca ctgggtggcc
     1621 gaacatctgc ccaaacaagc ggaccaatgg tttgcccagg cgcaggaaaa acccggttcc
     1681 tggtggctgg attgggatgc ctggctgcgg gctaattgct cggctacccg tgcggtggca
     1741 cctgtgtatg cagcggataa tccatctgcg ccgggggact acgtcaagcg tcgtttggag
     1801 acaatttaga gcggatgctg gccgggaaaa atcacgcgga tgcggcctgg gcagttcagc
     1861 cctggctata ctcattggac ttgaattgag gtaacaccca tgatcaccat taaaaaatat
     1921 cccaatcggc ggttgtacga cacctccaag agccagtaca tcaacctcga ggcaattaaa
     1981 ctgctggtga tgacggggca agaattccaa gtggtggatt caaaaaccga cgagaatctc
     2041 accaagagta ttttgttgca gattattacc gagcaagaag gcaatgaggg gcaggcgctt
     2101 ttgacgcaaa cggtacttaa gcagttgatc cggttttatg gcagcgatct gcagggtttt
     2161 atgagccagt acctagagca gagtgtggcg acctttttag atcgccagga ggctatgcaa
     2221 agtgtgatgc acgacttgct cgataaggcc tcgccgttaa atgtatttaa ccaacttatg
     2281 gagcaaaaca tgagtgtgtg gcgcagcttt gccaacccca tgggcaaatc tgctaacgag
     2341 cctaaggata aggaataatt ttattgcgtc gcagcaaaaa gagcttgacg ctcagggaat
     2401 cggcaagtag acttcagttg tattctgcaa tgcagcaact tgtggtacaa aggttctgct
     2461 aactatccac tgaggagata accatgtacg agaaattttt tgagcaagca caaactgcac
     2521 tgaagccctt tagcgaactg ctgactctga acgctaaaat tgccgaagaa gcagccgaga
     2581 agcaaaaaat cttcatcagc gaattggtca atgacagcgt ggcctttgct aaaactctga
     2641 gcgcccagaa agattattct ggcatttacc aaactcagaa gtcgtacctt gagggcctgc
     2701 aagctaagtg ggtggcagcc tctaccgatg cttaccagac gttcactacc actagtgagt
     2761 ctgttggcaa ggtgattaaa agcgccgcca cggtgcagta gttccaaaat catctgaaaa
     2821 agcctggcct agctgggctt tttttttgtc ttttgactga tcatggctta taaattaccc
     2881 cttagggatt ggtccggtgc acgcttggat gtgtctgtaa aaaagttgta attcggcagc
     2941 tgtagcagtt aagacgtaag tttcgtaccg ttaatcacga ggagatgctc catgtaccaa
     3001 caatttctgg atcaagctgg aacattgttc aaaccactgc aggatttggt gagccttaat
     3061 caaaccgctt ttgaggcgct ggccaacaaa caatcggcac tggtatctga aatcatcaac
     3121 gacagtattt ccttcaccca gaaagtgttg gcggaaaaag acggcaatta ctgggatttg
     3181 cacaaggctt attgggaggg ggtgaatcaa aaactcaacc aagccagcca agaggccgcc
     3241 gattatttca ccgcgacaca aaccaagctg agtgatttgg tgcaagagag cctgttgggc
     3301 agtgccatgg cggaggtgac caaacacatg ccttcggtgg aagcggctgt tgcctcagcc
     3361 ctaaagccta ttgagaccgc caaaattaaa cctgctgccg aagcggccaa ggtgcctgct
     3421 gctaagccag cacctgcacc ggccttggcc accaaaaagc cagcggcaaa acctgctgct
     3481 aagcctgcag ccaaagtggc tgatgcgaaa cctgctgaat ctaaaaagcc tgctgacgcc
     3541 aaaaaatcag ttgaagccga aaaaacagtg gaagtaaaaa gccccgtaaa accagcagaa
     3601 aaaccccccg ccgttaaagc gcccgcgact gaagaaaaat cgactcgggt ggcaggtgat
     3661 gatgcgacgg ttgaaagtgt aattcgctcg attatggctg agcgcaatca ctaaacacta
     3721 agtaaaatag ttgtgcgcgt ttgtggtagg gagttattaa cccggcggca ttgagtgccg
     3781 ggttacgggt tacgggttac gggttaattg tgatttcaaa ccagtgttgt ttacaccaag
     3841 gcaacgcgct taaaactatt cgacaatcaa aaaaattgcc ccttcagggg caattttttt
     3901 ggggctgtga ctttaagagt cgtatctagt ccttagtaca tgtgctggcc gccgtttatg
     3961 gacagggttg agccagtgat aaaacctgat tcatcggcca ctaaaaattc cacaccgcga
     4021 gcgatttcat ccgcctcacc caaacggccg acgggaatct tagctacgat tttttccagg
     4081 actttttctg gtacagcgcg caccatatcg gtggctatgt agcccggcgc tatagcgttt
     4141 acagtaatgc ctttgttagc gccttcttgg gcgagggctt tggtgaagcc gtggatgccg
     4201 gatttggcag cggcgtagtt cacttggccg tactgaccgg cttggccatt gatggagcct
     4261 atgttgacga tacggccgaa attgttctcg cgcatttgtt caatcacggc atggcacagg
     4321 ttgaagcagg agcttaggtt ggtgtcgatc accgcgtgcc aggcttcttt actcattttg
     4381 tgcagcgtgc tgtcgcgggt gatgcccgca ttgttaacta gaatttcaat ggggccaaaa
     4441 tcgcggcgga tttgctcaac agctgcggcg caggcgtcaa aatccgccac atcaaattta
     4501 tacaccggga tgttgtgctc agccttaaac gctgcggcgg cttcatcgtt gccggcatag
     4561 gctgctacta cgttgtaacc tgcttttttt aatcgaaggg aaattgcggc gccaatacct
     4621 cgagtgccac ctgttacaag agcgagtcga cccatttggg atctcctacc tagttaatag
     4681 aggcgacata ataggatggg aatggccgct tggcaaaaaa gcttgcgccc caacaaatgc
     4741 tgcaactgcg cgagccagta tcaattttgc ttagcgggct acttgtaatt gcggcgccga
     4801 gccgcaacta tttgtggctc atttctgcga gtgtgcccca tggaaacctg agtctcaatt
     4861 aatgcctcca acgccaaaca atatttaatt gacgaatcca tgcagcggcc ggtgctcatc
     4921 gatttttggg cggactggtg cgcgccgtgc aaaaccctca tgcccctatt ggagcggctg
     4981 gcaacggaat acgcgggagc ctttttgctc gccaaactca acgccgatga gcagcgcatg
     5041 atcgcctcac agtttggtgt gcagagtttg ccgacggtga tcttgatgaa agatggccag
     5101 cccatcgatg gttttgtggg ggccaaaacc gaagtggaga ttcgccagtt tttggataaa
     5161 cacctgccca aagcctggga tgggcaattt ctggaggccc aggctttgat ggcggcgggg
     5221 gattttaatg gcgccgtgag cctgttgcgc caagctcagc aggactccgg cgggcgggcg
     5281 gatatagtct gcgccctggc ggagtgttta attcatctta agcgcctaga tgaagcccaa
     5341 agtcttctgg cgcaggtgcg gttggtggat caggacgggc gtttccatca ggtgcaggct
     5401 ctgttggagt tggccagcaa cgctcagaag gcgccggaaa ttaccgacct tgaggccttg
     5461 ctggcggccg atcccgcaag cacggcactg gcccatcaat tggccctgca atattctcag
     5521 cacgggtaca ccagtgaggc cctggagctt ttatattcac tgctgaaaaa a
//
LOCUS       DAOQGS010000253         5548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7390_length_5548_cov_4.068451, whole genome shotgun sequence.
ACCESSION   DAOQGS010000253 DAOQGS010000000
VERSION     DAOQGS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_5548_cov_4.068451"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..823)
                     /locus_tag="PKZ52_10340"
     CDS             complement(176..823)
                     /locus_tag="PKZ52_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60213.1"
                     /translation="MQTPLYFTPYKDTRLDTIYNLQFCDNDTLLHSEYRPTGILAQLL
                     NAQVSNEILQTLAMNGDEDTRIRLLAFAQLRKNNIAVPQKILLATIVEVGLADGLEML
                     TVYDDRRVRYISAKGRLAVVESPTPPMELIIQGFLADSQKIINHLNPCDDARHTFPSY
                     GRAKMTFIASDGIYIGEAQFKHMQENPEAAKIIDGALDLLHEIIITGNVSPNDLI"
     gene            complement(979..1257)
                     /locus_tag="PKZ52_10345"
     CDS             complement(979..1257)
                     /locus_tag="PKZ52_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60214.1"
                     /translation="MSNSCELCGRQVALTDHHLIPRMLHRRKRYQNNFSRAELTLGTI
                     ALCRLCHRGIHALYDEVTLAEQFNTLAKLKADPAIAKHCSWVAKQKQL"
     gene            complement(1254..1658)
                     /locus_tag="PKZ52_10350"
     CDS             complement(1254..1658)
                     /locus_tag="PKZ52_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60215.1"
                     /translation="MRGAGGTSGGVGQFFLGFAMMCAGFYLLFNAINVYSSFGLGSVL
                     FQIPVGSYQAGISSGMILIPFMIGIGIIFYSASNVIGWLLAIGSLAALIVGVISSLHF
                     SLRTMSAFELIAILVLAMGGLGLFLRSLRSFE"
     gene            complement(1668..2438)
                     /gene="tatC"
                     /locus_tag="PKZ52_10355"
     CDS             complement(1668..2438)
                     /gene="tatC"
                     /locus_tag="PKZ52_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNG60216.1"
                     /translation="MTDPIKEEQESQPLVTHLIELRDRLLRCIYATLGLFLAMAYFSN
                     RIYAFITEPLTALLREFNEGTLIVTDVTGSFVAPLKLTFYIALFVTMPYIIYQIWAFI
                     APALYRHERRLAIPLFISSVILFYIGAAFANFLIIPGAFKFFMLFAPANVTVNPDITM
                     VLDFIIKVSLAFGVAFEIPIAVVILLVTGVTTAESLIAKRSYVIVACFVVAMFLTPPD
                     VMSQFMMAIPLWALFECGIYFGRWIVNTRPKDDVPDYD"
     gene            complement(2431..2916)
                     /gene="tatB"
                     /locus_tag="PKZ52_10360"
     CDS             complement(2431..2916)
                     /gene="tatB"
                     /locus_tag="PKZ52_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNG60217.1"
                     /translation="MFDVSFFELLVIGVVALVVIGPEKLPGAIRTCALWIGRMKRILA
                     ESRSEFEKHIGADEIRRELHNEQIMASLEKLKASRAILEQDLLNYQNTLQAELNALEH
                     GAEVTPSEHTQESAPHSGTSDTELTSAEHHSNTTTAEIPSGHSDTLPHDSAPITKSTH
                     D"
     gene            complement(2927..3190)
                     /gene="tatA"
                     /locus_tag="PKZ52_10365"
     CDS             complement(2927..3190)
                     /gene="tatA"
                     /locus_tag="PKZ52_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNG60218.1"
                     /translation="MFSGAFSIGHLIVVLVIVLLLFGTKKLKNLGSDLGGAIKGFKNA
                     LNEGEKKEADTSEIDATKKVTEEKSTQQADLNKVSEPAKSEQK"
     gene            complement(3220..3555)
                     /locus_tag="PKZ52_10370"
     CDS             complement(3220..3555)
                     /locus_tag="PKZ52_10370"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNG60219.1"
                     /translation="MNDTLQQLTQVLAERRRAADPSSSYVAQLHHKGLNKILQKLGEE
                     CTETIIAAKDAQHTGDTQALIYETADLWFHSLVMLSHLNLSADDILAELARRFNVSGI
                     AEKNARPQQ"
     gene            complement(3565..3948)
                     /gene="hisI"
                     /locus_tag="PKZ52_10375"
     CDS             complement(3565..3948)
                     /gene="hisI"
                     /locus_tag="PKZ52_10375"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNG60220.1"
                     /translation="MPDYLSEIRWTADGLVPVIAQDVQSGKVLMMAWMNRDALQATVE
                     TNRAVYWSRSRKALWHKGETSGHEQIVHSIDLDCDADVLILSVEQQGGIACHTGRQSC
                     FYRRLVDGQWQIQDPVLKDPNEIYQ"
     gene            complement(4290..>5548)
                     /locus_tag="PKZ52_10380"
     CDS             complement(4290..>5548)
                     /locus_tag="PKZ52_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG60221.1"
                     /translation="VTEVKMFDHRGKNLASAVKSGTADFSTKVGRNGIEIVREKEVIG
                     RYDIVFSTQELDNLLVSQLRSSAIVIVSLLLASLATVFVLSRSLIVSPITSISESLDA
                     IAAGGGDLTRRLPTTSGDEVAVLSNNFNRVMEQIGNIIKNVTQVTQKVNNNVRIMSGA
                     TENTVHSTGQQLKEIELVAAALQELTHSATEVARHAGATADHTKETARFAEQGSQVVK
                     SSRDTGNRLTDQIQATARKISILKENSENIGSVMEVIRSIAEQTNLLALNAAIEAARA
                     GEQGRGFAVVADEVRSLAQKTQTSTEEIGSIIVQLQRAADEAHQSMNTSMSSVQETIE
                     TSTKVGESLERIRTNIDTINNMNHQIATAAGEQSSVANEVSKNITAIHTLSERVAENA
                     RVVSANSDQLKHESSELKQQMDKFNI"
BASE COUNT         1387 a         1250 c         1461 g         1450 t
ORIGIN      
        1 ggtgggcggc ggggccttgg tggggtaatt attttatgtc catcgaaaaa cctgccaacg
       61 gcgatgcacc gcaaatgtct tggttggcgc cgttcttaaa ataagaatcg cttaaaaata
      121 aaaaccggct ggtggtaaca ccagccggtt ttttttgtgt ttgggatcct gcgttttaaa
      181 ttaaatcgtt tggcgataca ttgccggtga taattatttc atgcagcaaa tccagggcgc
      241 cgtcgataat cttggcggcc tctggatttt cttgcatgtg tttaaattgt gcctcgccga
      301 tataaatacc atctgaagct ataaaggtca ttttggctct gccgtagctt ggaaaagtat
      361 ggcgagcatc gtcgcaagga tttaagtgat tgataatttt ttgcgagtcg gcaagaaacc
      421 cttgaataat taattccatg ggtggtgttg gtgattccac aaccgccagg cgcccctttg
      481 cgctgatata gcgtacccgc cggtcgtcgt acacggtaag catctctaaa ccgtcggcaa
      541 gccctacctc tacaatggtg gctagcagaa ttttctgcgg tacggcaatg ttgtttttgc
      601 gcaattgtgc gaatgccaat aagcgaatgc gcgtatcttc atcaccattc atcgccagcg
      661 tttgcagaat ttcgttactc acttgtgcgt tcagtaattg cgccaaaatt cctgtagggc
      721 gatattcgct gtgcaacaag gtatcgttgt cgcaaaattg caggttgtaa atggtgtcga
      781 gccgcgtgtc tttgtacggc gtaaagtaaa gtggcgtttg cataagcgat gggaccgggt
      841 tgctttgctt gagtataggc aagtcttctg gcccggccag gtcagtcttc gcaatagaac
      901 aaaaacacct ataggcacaa ataatcggta aaaattaagg tgtcactgca ggcagattgt
      961 gaatgattca ggagaatttt ataactgttt ttgtttggcg acccagctgc aatgttttgc
     1021 aatcgcaggg tccgctttaa gttttgccag ggtattaaac tgctcagcaa gggttacttc
     1081 atcgtacagt gcatggatac cgcgatggca gaggcggcat agggcgatag tgccgagggt
     1141 gagttcggcc cgtgaaaagt tgttttggta gcgcttgcgc cggtgcaaca tgcgcgggat
     1201 gaggtgatga tcggtaaggg ccacctggcg gccgcaaagt tcgcagctgt tactcactca
     1261 aatgagcgca gagaccgcaa aaacaaaccc agcccgccca tggccagcac gagaatggct
     1321 attaattcaa aggcggacat ggtgcgcagg gagaagtgca aggagctgat gacgcccacg
     1381 ataagggctg ctaatgagcc tatggccagt agccagccaa tcacattgct agcgctgtaa
     1441 aagatgatgc cgataccgat cataaatgga attaaaatca tgccggatga tataccggcc
     1501 tgatagctac ctacagggat ttgaaagagc accgagccta agccaaacga tgaatagaca
     1561 ttaatggcat taaacagcag gtaaaaacct gcacacatca tggcaaagcc taaaaaaaac
     1621 tggcctacac cgccagaggt tccacctgca ccgcgcatgg ctaactccta atcgtaatcg
     1681 ggcacatcat ctttggggcg agtattcaca atccagcggc cgaagtaaat gccgcattca
     1741 aacaatgccc aaagcggaat agccatcata aattgcgaca taacatcagg tggggtgaga
     1801 aacatggcga caacaaaaca cgccacaatc acgtaagagc gtttggcaat taaggattcc
     1861 gctgtggtaa ccccagtcac cagaagaata acaacggcga tgggaatttc aaatgctacg
     1921 ccaaatgcta aagaaacctt aatgataaaa tccaacacca tggtgatatc ggggttgacg
     1981 gtgacgttag caggtgcaaa taacataaaa aatttaaatg caccgggaat aattagaaag
     2041 ttggcaaatg cggcgcctat gtaaaacaaa ataacactgg atataaacag cggtatggcc
     2101 aggcgccgtt cgtggcgata gagggcaggg gcaataaatg cccagatttg atagatgatg
     2161 tagggcatgg taacgaagag cgcgatataa aaagtgagct ttaaaggcgc cacaaaagag
     2221 ccggttacgt ccgttacaat taatgtacct tcgttaaact cccgcaatag ggcggttaaa
     2281 ggttctgtaa taaaggcata aatacggttg gaaaaatatg ccatggccaa aaacaaacct
     2341 agggttgcgt agatacaacg caacagccga tcccgcagct caatgaggtg agtgactagg
     2401 ggctgagatt cctgttcctc ttttattggg tcagtcatgt gtggatttcg ttataggggc
     2461 agaatcatga gggagtgtgt cggaatgccc agagggtatc tcggcagttg tggtgttgct
     2521 gtggtgctca gcacttgtaa gttctgtatc gctcgtgcct gagtgcggcg cggattcctg
     2581 tgtatgttcg ctaggtgtga cttctgcgcc gtgctctaaa gcgtttaatt cagcttgcag
     2641 ggtattttga taattgagca gatcctgctc caatatggcc cgcgatgcct tgagtttttc
     2701 caaggacgcc atgatttgtt cgttgtgcaa ttcccggcga atttcatcgg cacctatgtg
     2761 tttttcaaat tccgagcggg attcggcgag aatacgcttc atgcggccaa tccacagggc
     2821 acaggtgcgg atggcgcccg gcaatttttc cgggccgatc acaacaagtg ccactacgcc
     2881 aataaccagt agttcaaaaa aactgacgtc aaacatactg agctatttat ttctgttcag
     2941 atttagcagg ttcactcact ttatttaaat ccgcctgctg ggtggatttt tcctcggtta
     3001 cctttttggt ggcatcaatt tcgctggtat cagcctcttt cttttcgcct tcattaagcg
     3061 cgtttttaaa gcccttgatg gcgccgccta agtctgaacc gagatttttt aattttttgg
     3121 ttccgaataa taaaagtaca atcaccagta caacgataag gtgacctatg ctgaaagcac
     3181 ctgagaacat aaaaacctct ttattgtttg aataagaatt tattgctgtg gacgggcgtt
     3241 tttttctgca atgcccgaga cgttaaagcg ccgcgcaagt tcggctaaaa tatcatcagc
     3301 ggacaaattt aagtgggata acatcaccaa actgtgaaac cacaaatccg ccgtctcata
     3361 aataagtgct tgcgtatcgc cagtgtgttg ggcgtctttt gcggcgatga tggtttcggt
     3421 gcactcctca cccagttttt gcaggatttt attcaaaccc ttgtggtgca gctgtgcaac
     3481 gtaggagcta ctgggatcgg cagcgcgcct gcgttcggca agaacttggg tgagttgctg
     3541 cagcgtatca ttcattgcat gtgctcattg gtaaatttcg ttagggtctt taaggaccgg
     3601 gtcttgtatt tgccactggc catccactaa gcggcggtaa aaacacgatt ggcggccggt
     3661 gtggcaggca ataccgccct gttgttcaac gctcaggatg agcacatcgg cgtcgcagtc
     3721 caaatcaata ctgtgcacaa tttgttcgtg gcccgaggtt tcgcccttgt gccagagggc
     3781 tttgcgtgag cgggaccaat agacggctcg gttggtttca accgtggcct gtaatgcgtc
     3841 gcgattcatc caggccatca tcagtacctt gcccgattga acgtcctggg cgatcaccgg
     3901 tactaggcca tcggcggtcc agcgaatttc actaagataa tcaggcatat cgaagaccgg
     3961 tcgttaaaaa aggcaagtag tctagcggat gccatggtat gtggctaccg cctaagggcg
     4021 gtttgccaaa gctcggttgg tgagattgcg cagattgtgg ggcggcgctg aaaaccactg
     4081 ccacctaggt ttagccgtgg ggagggcaga gaagggtttg agcctgcaaa gacgcaaaaa
     4141 ccagcttggc attgagcgaa gctggttgct tgagactgaa taattgctag aggatgagtt
     4201 ggcggaagta atcaatagcg tttgtcagag acatcccgag tagatcctgc ccaggctcgc
     4261 cccaaaagcc tacccatttc aacacgctgc taaatattaa atttatccat ttgttgtttt
     4321 aactccgagc tttcatgttt gagctgatcg ctattggcgc taacaacacg ggcgttttca
     4381 gcaacccgct cggatagggt gtggatggcg gtgatatttt tgctcacctc attggctact
     4441 gagctttgct caccggcggc agtggcgatt tgatggttca tgttattgat cgtatcaata
     4501 ttagtgcgaa tacgttcgag ggattcgccc actttggtgg aggtttcaat ggtctcctgc
     4561 actgaggaca tactggtatt catagactga tgggcctcat ccgccgcacg ttgcaattgc
     4621 acgatgattg agccgatttc ttcagtggag gtttgagttt tttgcgccag tgagcgcact
     4681 tcatcggcaa ccaccgcaaa gccccggcct tgttcgccag cgcgggccgc ttcaatggca
     4741 gcgtttaacg ccagtaagtt ggtttgttcg gcgatggagc gaatcacttc catcaccgag
     4801 ccgatatttt cagagttttc tttgaggatg gaaattttgc gggcggtggc ttgaatttgg
     4861 tcggttaagc gattgccggt atcccgtgag gatttaacca cttgcgagcc ctgctcagca
     4921 aaacgcgcgg tttctttagt gtggtcggca gtggcacctg catgacgggc cacttcagtg
     4981 gctgagtggg ttaattcctg taatgccgcc gcgactaatt caatttcttt taactgttga
     5041 ccggtggagt gcacggtatt ttccgtggca ccgctcatga tgcgcacgtt gttatttacc
     5101 ttttgagtta cctgggtaac atttttaata atgttgccaa tttgctccat tacgcggtta
     5161 aagttattgg ataacacggc gacttcatcg ccgctggtgg tgggcaagcg gcgggttaga
     5221 tcgccgcccc cggcagcaat ggcatctaag gattcgctga tgctggtaat gggcgagaca
     5281 attaggctgc gggataacac aaatactgtg gcgaggctgg ccagtagcag gcttacgata
     5341 acaatggcgc tggagcgcaa ctggctaacc aataaattgt cgagttcctg ggtggaaaac
     5401 actatgtcgt agcggccaat aacttctttt tcgcgcacaa tttcgatacc gttgcgcccg
     5461 accttagtag aaaaatccgc tgtacccgat ttgaccgcgc tggctaagtt tttgcctcgg
     5521 tggtcgaaca ttttcacttc ggttacta
//
LOCUS       DAOQGS010000254         5540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7409_length_5540_cov_4.477484, whole genome shotgun sequence.
ACCESSION   DAOQGS010000254 DAOQGS010000000
VERSION     DAOQGS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7409_length_5540_cov_4.477484"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..147
                     /locus_tag="PKZ52_10385"
     tRNA            60..147
                     /locus_tag="PKZ52_10385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94..96,aa:Ser,seq:tga)
     gene            445..849
                     /locus_tag="PKZ52_10390"
     CDS             445..849
                     /locus_tag="PKZ52_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNG60222.1"
                     /translation="MNRSDMAHPWGEHQPIYRQLRERIVGLLLSGTLSEDQALPSIRQ
                     LASECQINHLTVSKAYQELVDMGLVEIRRGMGMYVAKGAAEQLRLLERQKFMTMELPQ
                     LIKRAAQLGISSDELCKALHSHTTQLTQAQEN"
     gene            850..1704
                     /locus_tag="PKZ52_10395"
     CDS             850..1704
                     /locus_tag="PKZ52_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG60223.1"
                     /translation="MNWVIQGQNLSKQYGQKNVLDGIDLTIAPGRIVGLIGPNGAGKT
                     TLLKGILGLTPIEGELRVLGYNPYRQRTAMLEKVSFIADTAILPRWLKVSEALTYVEG
                     VHPKFNRSKALDFLAKTQIQPAQTIQNLSKGMITQLHLALIMAIDSQLLVLDEPTLGL
                     DILYRKQFYESLLNDYYDAQKTLLITTHQVEEIEGMLTDILFINQGKITLNSPMEDLP
                     KRFAILQAEPDKVETAKALNPIYSRSLLGSDYLLFENPDFAALTSLGRLSTPSLADVF
                     VAKMQNCH"
     gene            1716..2654
                     /locus_tag="PKZ52_10400"
     CDS             1716..2654
                     /locus_tag="PKZ52_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60224.1"
                     /translation="MRKLFTLIRRELWEYKTGFITLPLLCTLILSLLLSVGFLRFAPP
                     PADLSAATATQAPIPNTPDQTSGDLNQQFSAYSKMPLNELPLFSHMDALRSAVGFLGF
                     TQWIVAVVLNFILLIATLNYAHRALFDDRKTREILFWRSMPVSEAENVLAKLSIIYGL
                     APLMLLGMSFLGGVISWLTAIGHGTQDALPALTKLLDAFSVYTKSLLFLFALLPIITW
                     TLLASAYAKKSPFLLSTFLPLGLILIDRLVHWATGINLYIHSSIGAYTDYLSHFLAAI
                     NNGLTLNHLSASIVIILVSTVFTLTAVWLRNNRYEI"
     gene            2912..4210
                     /locus_tag="PKZ52_10405"
     CDS             2912..4210
                     /locus_tag="PKZ52_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60225.1"
                     /translation="MRAHPTRFSLLSEQTSLAKLLSVCVVTAAVTACSGPTGDSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSNSSSSSSSSSSSGRSSSSSSSSSSSSSGTAIVTP
                     SEWSRCSACHGTNGKNGDIEIDVNFLGNNLESEIATMPPTGTKCTGTCNTNVAKFLRA
                     VNGISTSSSSSSSSSSSSSSSSSSSSSSSGSGVGAILFEDSFEFTSLGQFPKGWNYFV
                     EYQQNPGHISPTNYITVVSDAKIGNRALLIKGGQSPSMITHALPAGLNKVYMRAWVKF
                     TRKLGQNPGANHETLMGIRGEVGSANNEIRFGEIKGVIGTNEVPSDNIAPKQDQWGKG
                     PVLAANTWHCIEVAFKGDMAYNTLTAYANGNLVHQITAASDWQNGALSANWMSGKFKE
                     VIFGWHSFSGISNQVTIDDVVVSKDAIGCDYTVGSGPAVQ"
     gene            complement(4350..>5540)
                     /locus_tag="PKZ52_10410"
     CDS             complement(4350..>5540)
                     /locus_tag="PKZ52_10410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG60226.1"
                     /translation="ETINNIVNLINQQTTIDGLVDIMLQESIGLLDTTDLGAFWLLDE
                     THNEFKLISVHNRNDSICEIRGDIDAIAVARCCAAADLFVLQPADYPIFMAHRGEIDA
                     ALVVAIRIEGRLTALLALVNQSGSGQFDPKMLQALGRLQIHAVSAVSKARMLEVLKMQ
                     NTRLEQTSFTDQLTGLSNRRHLIKNIDSDLVLCSRKYESAKSSGQKPFNADMIFMIID
                     IDHFKMVNDTYGHNAGDALLKQFSGLLKSVFRETDYIVRWGGEEFMVVVRFCERTDAA
                     LLAERFRALVEQTPFEIGNDLILRKTCSIGFSCFPFYPDLDEHITWEQVADIADKALY
                     AAKKTTRNAWVGVWAQQAHAKQLPYAHLIACPELFEDMEGICVQTSIAADQPLIWNEL
                     CVPK"
BASE COUNT         1504 a         1424 c         1169 g         1443 t
ORIGIN      
        1 cctacccaga aatttgttgc tccaaaagat aaaagctcta tataatcgcg gcctcatttg
       61 gaggggtggc tgagtggcct aaagcggcgg tcttgaaaac cgttgagtgt aaaagctccc
      121 agggttcgaa tccctgcccc tccgccatat taaaacctcg catcgcgagg ttttttttcg
      181 cccctaaatt ggcaattgct atagctcaaa catgagcatg cccagccgtg tcgcgcttca
      241 gttgcccttc aacacccatt agtttctccg gccgacgaca attactattt tcaatcccaa
      301 ctaaccactt aacacccgca agcagctagc gtgtagggaa acacaaccta aggctctatt
      361 gccgcgccgc caaagccatt tacttaagtc accccgcagt gttatggttt tacataacac
      421 taaaacacct aagggacttc cagcttgaac cgctcagata tggcccaccc ctggggtgaa
      481 catcagccga tttacaggca attgcgcgag cgcattgtgg gcttgctgct gtccggcaca
      541 ctctctgaag accaagcgct accgtctatt cgccagctcg cttccgaatg ccaaattaat
      601 catttaaccg tcagcaaagc ctatcaggag cttgtggata tgggactggt ggagattcgc
      661 cgcgggatgg gcatgtatgt ggccaaaggt gcagccgagc agttaaggct cctggagcga
      721 cagaaattta tgactatgga actgccacaa ctgattaaac gcgcagcgca attgggtatc
      781 agcagcgatg aattatgcaa agcgctccac agccacacaa cacagctaac tcaagcacag
      841 gagaattaaa tgaactgggt aattcagggg cagaatttat ctaagcagta tgggcaaaaa
      901 aatgtccttg atggcattga tttaaccatt gcacctggcc gaattgtagg ccttatcggc
      961 cccaatggag cgggtaaaac aaccctctta aaaggcattt tagggcttac acctattgag
     1021 ggtgaattgc gcgtattggg ctacaacccc taccggcagc gcaccgccat gcttgagaag
     1081 gtttctttta ttgccgatac cgctatttta ccgcgctggt taaaagtatc tgaagcatta
     1141 acctatgttg aaggcgtgca ccctaaattt aatcgcagca aggccttaga ttttttagcc
     1201 aaaactcaaa ttcaaccagc ccagaccata caaaatctta gcaaaggcat gataacgcag
     1261 ttgcatttag ctttaattat ggccattgac agccaattat tggtactgga tgaaccgacc
     1321 ctaggcttgg atattctcta ccgcaaacaa ttttatgagt cgctcttaaa cgactattac
     1381 gatgcacaaa aaactctact gattactacc catcaagtag aggaaattga gggcatgtta
     1441 acggacatcc tttttatcaa tcagggcaaa attaccctta atagccccat ggaagactta
     1501 cccaaacgct ttgctatttt acaagcagaa cctgacaaag ttgaaaccgc caaggcatta
     1561 aaccctattt atagccgcag cctattgggt agtgactacc tattatttga aaaccccgac
     1621 tttgctgcac tcacctcact tggccggtta agcacaccca gccttgccga tgtgttcgtt
     1681 gccaaaatgc aaaactgcca ttaaggacat ccccaatgag aaaacttttc acattaattc
     1741 gccgggaact ttgggaatat aaaactggct ttatcaccct ccccctcctg tgcacattga
     1801 ttttaagcct gttattaagc gtgggatttc tgcgttttgc gccaccgccc gcagacttat
     1861 cggcagcaac ggccacgcaa gcgcccattc ccaacactcc cgatcaaacc agcggcgatc
     1921 tcaatcagca attctctgcg tatagcaaaa tgccactaaa tgaattgccg ctgtttagcc
     1981 atatggatgc gcttcgatcc gctgtcggat ttctaggatt tacgcaatgg attgtcgctg
     2041 ttgtactgaa ttttatattg ctcatagcaa cactaaatta cgcccaccgc gctctatttg
     2101 atgatcgcaa aacccgcgaa attctcttct ggcgctccat gcccgtgagc gaagcggaaa
     2161 atgttctagc aaaattatcg ataatctatg gccttgcgcc attgatgttg ctgggcatgt
     2221 catttttagg gggcgttatt agttggttga ccgcaatcgg ccacggcaca caagacgcac
     2281 tcccagcgct caccaaactg ctcgatgctt tcagtgttta tactaaatct ctgctgtttt
     2341 tatttgcatt attgccgatc ataacctgga cactacttgc ctccgcctat gccaaaaaat
     2401 ctcctttttt actcagcact tttttgcctt tagggctgat attgattgat cgcctcgtgc
     2461 actgggccac aggtattaat ctatatatcc acagcagcat cggtgcctac accgattacc
     2521 tatcgcactt tctcgccgcc attaataatg gccttacact caaccacctg agcgcatcca
     2581 ttgtgatcat tttggtcagc acagtcttca ccttgacggc tgtttggctg aggaataatc
     2641 gctatgaaat ttagccgcag ctattaacca ttttgctcat ccaacacttc tagcaagggg
     2701 aagaaaattc cccattttta aaaatcatag tttgaggttt tggcctattt tggggcgcac
     2761 atacaacacc tgaataacag ccctagcagg cgtgaatgca aacagagtca cagcgtaaaa
     2821 gcaggcctag tggtaaagta aaccattaga agctgtgtgg aataacaaac agccttaaat
     2881 caacctttgg ttctaacttc ggtatttact aatgcgcgca catcccactc gattttcctt
     2941 gcttagcgaa caaaccagcc tggccaaatt attatctgtc tgtgttgtaa ccgctgctgt
     3001 cacagcctgt agcggcccca ccggcgactc cagctcatcc agctccagta gttcttctag
     3061 cagctcctcc agtagctcat catcaagcag ctcctcaagc tctagtagtt catccaactc
     3121 cagcagttca tctagctcaa gcagctcctc tggccgcagc agctcatcat ccagctctag
     3181 ctcatcatcc tcatctggca ccgcgattgt cacgccctct gagtggtcgc gttgctctgc
     3241 atgtcacggt accaatggca aaaacggcga tattgagatt gacgtaaatt tcctgggcaa
     3301 caatttggaa agtgaaattg ccaccatgcc acccactggc accaaatgta ccggcacctg
     3361 taacaccaac gtggctaagt tcctgcgcgc agttaatggc attagcacat cgtcaagttc
     3421 ctctagctct agctctagct cgtcaagctc cagctcatca tccagctcca gttcttctgg
     3481 ctcgggcgta ggtgccattt tgtttgagga cagcttcgaa tttaccagcc tggggcaatt
     3541 ccccaaagga tggaattact tcgttgagta tcaacaaaac cccggtcaca ttagtccaac
     3601 aaattacatt accgtagttt cggatgccaa aatcggcaat cgcgcactgc tcattaaagg
     3661 cggtcaaagt ccttctatga tcactcatgc gttacccgca ggattaaata aagtgtacat
     3721 gcgcgcctgg gttaaattta ctcgcaaact gggtcaaaac ccaggcgcca accacgaaac
     3781 gctgatgggt attcgtggcg aagtgggcag cgcgaataat gaaattcgtt ttggagaaat
     3841 caaaggtgtt ataggtacta acgaagttcc atcggacaac attgcaccaa aacaagatca
     3901 atggggcaaa ggtcctgtgc tagcggctaa cacatggcat tgtattgagg tcgccttcaa
     3961 gggagacatg gcttacaaca cccttactgc ttatgcaaac ggcaacttgg tacatcaaat
     4021 taccgccgca agtgattggc aaaacggtgc gctttctgcc aattggatga gcggtaaatt
     4081 caaagaagtg atctttggct ggcacagctt tagtggcata tccaatcaag tcaccatcga
     4141 cgacgtagtt gtatctaaag atgctatcgg ctgtgattac accgttggat ccggccctgc
     4201 cgtgcaataa aatttctaaa attcaatccg ctcaataaaa aagccctctt atgagggctt
     4261 ttttgttttt gaatcttttc aacgctggcc ctcaaaaccc aatcacacac gagcctattc
     4321 ttcttaatca gaaattgccg caattaccat tactttggca cacacaactc attccaaatt
     4381 aatggctggt ctgcagcaat agaggtttgc acacaaatac cttccatgtc ctcaaataat
     4441 tcagggcacg caattaggtg ggcatagggt aattgtttgg cgtgggcctg ctgcgcccaa
     4501 actccaaccc aggcattgcg agtggttttt ttcgcggcgt acagggcttt atcggctata
     4561 tcggccacct gctcccaggt gatatgttca tctaagtcag gataaaatgg aaaacatgag
     4621 aaaccaatgg agcaggtttt acgcaggata agatcattgc ctatttcaaa tggggtttgt
     4681 tcgactaagg ctctaaaacg ctcggcaagt aatgcagcat ccgtgcgctc gcaaaaacgc
     4741 accactacca tgaactcctc accaccccaa cgcacgatgt aatcggtctc gcgaaatact
     4801 gattttaaca aaccggaaaa ctgctttaac agagcatccc cagcgttgtg cccataggta
     4861 tcgttaacca ttttaaagtg gtcgatatcg ataatcataa aaatcatatc ggcattaaac
     4921 ggcttttgcc cagagctttt tgccgactcg tatttgcgcg aacacaaaac caaatcgcta
     4981 tcaatatttt taatcagatg gcggcggtta ctcaggccgg ttaattggtc ggtaaagcta
     5041 gtttgctcta aacgcgtatt ttgcattttt aatacttcaa gcatacgcgc tttggatacc
     5101 gccgataccg catgaatttg taggcggccc aatgcctgca acattttagg gtcaaattgg
     5161 cctgagccgc tttggttaac caaagccagc agcgcggtta acctaccctc aatacggatc
     5221 gccaccacta aggcagcgtc aatctcgcct ctatgggcca taaaaatggg gtaatcggct
     5281 ggttgcaata caaataaatc ggcggcagcg cagcagcggg ctacggcgat tgcgtctata
     5341 tcccctctga tttcacaaat ggagtcgttg cggttgtgaa ctgaaattaa tttaaattca
     5401 ttgtgcgttt catccaacaa ccaaaatgcg cctaaatctg tggtatcaag taagccgatg
     5461 gactcctgca acataatatc caccaaacca tcgatggtgg tttgctggtt aatgaggtta
     5521 acgatattgt tgatagtctc
//
LOCUS       DAOQGS010000255         5493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7516_length_5493_cov_5.443729, whole genome shotgun sequence.
ACCESSION   DAOQGS010000255 DAOQGS010000000
VERSION     DAOQGS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7516_length_5493_cov_5.443729"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKZ52_10415"
     CDS             <1..1179
                     /locus_tag="PKZ52_10415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNG60227.1"
                     /translation="SLSLLPADPILGLMALYAQDKNPQKVDLGVGVYKTEEGHTPVLR
                     AIKAAEAALIAEQNSKVYVGPGGNEGFLQALSEMVFAEHLPGINERLAWAQTPGGCGA
                     LRVAAEIIKKARPGACIWVSDPTWGNHIPLLGNAGLEIKTYPYYDATSHGVNEAAMLE
                     CLAQVPAGDLVLLHGCCHNPTGVDLSLAQWHSIGALAARQGFVPFVDMAYQGFGVSLE
                     DDAKGLQSLLTSVPEVVLALSCSKNFGLYRERVGAVAVLAANRTTATAIKSHMMAIVR
                     GIYSMPPDHGAALVAAVWQNPHWQTLWRQELDEMRQRIAQLRQDFVTGMNARGAAGRF
                     DFILAQFGMFSFLGLSAKQVQELMSRYSIYLLSNSRASIAGLNRANLDYVCDAIIKVV
                     "
     gene            1366..2580
                     /locus_tag="PKZ52_10420"
     CDS             1366..2580
                     /locus_tag="PKZ52_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNG60228.1"
                     /translation="MASSLQKMYVSDLRVGMYVAKLDRDWLETPFILQGFVLESQEDV
                     DIVAEYCSYVYVDTVYKALATPAASGTRGKGPIQYQNTTRIDEEHRKIVGAFCQARTL
                     TKSLLDDIRLGGAINTDHAKQMVSDCVQSVIRHPDALMWMAKIRSESEYTAEHCLNVC
                     ILAVAFGRVLGMTEEELHKLGMCGLLHDVGKMKVPPDILNKPASLTLGEMRTMMAHTI
                     HGRNLLLSTDGIYNGVIDVAYSHHERVDGKGYPRKLAGASISLFSKIIAIVDAYDAMT
                     ADRCYQSAKTSTEALKIIYQDRGSHFDERLALKFLQTVGLYPPGSIVELHSGQVAIVV
                     ETNHKLRHLPKVLLVRDQNKKPLEKELFMDLSQIEEGTLPQEYLIKQVWKDRSFGISL
                     RKYIEQGLVLNR"
     gene            complement(2722..3720)
                     /locus_tag="PKZ52_10425"
     CDS             complement(2722..3720)
                     /locus_tag="PKZ52_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNG60229.1"
                     /translation="MKTYDIYGLGAALVDTEITVQDDNLREFNIAKGVMTLVDEARQH
                     ELIRLLDGHLVKAKRASGGSAANSIIAAAQFGSSCFYSCKVADDDNGHFYLQDLSRAK
                     VAYPACARAAHGITGKCLVMITPDAERTMNTFLGISASLSTADLDFDSLAKARFAYIE
                     GYLVTLESGRPAAITLREAAQKLGVRTALSLSDPAIVSYFHDGLTAMIGSGVDILFCN
                     EAEALNFTRTTHLDAAFEALKGIAKTFAVTCGAKGAWVFDGEQLQLVPSQPVQALDSN
                     GAGDMFAGAFLHGIGRGWNYFEAAAFANRAAGLVVSQYGPRLAAEQHQQLLAYAPR"
     gene            4014..>5493
                     /locus_tag="PKZ52_10430"
     CDS             4014..>5493
                     /locus_tag="PKZ52_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNG60230.1"
                     /translation="MSRLIRDDKNTKGAEAVLPEVQLPLNRETVIAHLKRCQQVVNEC
                     ARQAFNRYAERLDEAFNIAIDNARSNQEVAEMAGVQRLVRKNRLELERYFTGYIAEGF
                     VKFKNKELRTDLSSTNRTSELSLVDNEDLDEMIVLTSITSKMDSYFAEPIWALNQRFA
                     ILNGGEQVTEAGNPAAPIQLCESLRRALRLIPLTAKAKTICYRTYEEHLTGFVGSVVD
                     ELNAYFKRSGILPNLKYMPPASSRGAINIGNTEFSPQDLAFMQSLGSPEHQTELLQAI
                     RGLQASLGGVQPLPEGVAMLSTEQLVNVLQSIQLATNLPNLAEGQALAPVNIAQSTQH
                     LAEQVKQQGGDVSQVDMHTIDLVGMVFEYMLSDENLPDSVKALLSYMHTPFLKLAFSD
                     PGFFERPEHPARVLLNSLAEAGTRFVGNDGSVQYDMYQKIKDVVDRVIKDFNNDVNVI
                     AELFIEFNSYTKNIIRRQELMERRATERVQGEERLREVKIRVN"
BASE COUNT         1303 a         1353 c         1518 g         1319 t
ORIGIN      
        1 tccttatctt tattgcccgc agatccgatc ctgggtttga tggcccttta tgcccaagat
       61 aaaaatccac aaaaagtaga cctgggtgtt ggcgtgtata aaacagaaga gggccacacg
      121 ccggttttgc gcgccattaa agctgctgaa gctgcattga ttgccgaaca gaattccaag
      181 gtgtatgtgg gccccggcgg caatgaggga tttttgcagg cattgtcgga aatggttttt
      241 gccgaacatc tgcccggtat aaacgagcgc ctcgcctggg cgcagacacc gggtggctgc
      301 ggcgccctgc gcgtggcggc ggaaattatt aaaaaagccc gccccggcgc ctgtatttgg
      361 gtgagtgatc caacctgggg aaaccatatt ccgctgttgg gtaacgccgg tttggagatc
      421 aaaacctacc cctactacga tgcaacaagc cacggtgtta acgaagcagc catgctggag
      481 tgtttagcgc aggtgcccgc aggcgactta gttttattgc acggctgttg ccataacccc
      541 accggggtgg atttatcctt ggcgcaatgg cacagcatag gtgcactggc cgcgcggcag
      601 ggctttgttc cctttgtgga tatggcttac cagggttttg gtgtgagttt agaagatgac
      661 gctaaaggat tgcagagctt gttaacgagt gtgccggaag tggtgttggc gctctcctgt
      721 tcaaaaaact tcggcctcta ccgcgagcgc gtgggggcgg tggcagtgct cgcggccaat
      781 cgcacaacgg caactgccat taaaagccat atgatggcga tcgtgcgcgg tatttattcc
      841 atgccgccgg atcacggtgc cgcccttgtg gcggccgtgt ggcagaaccc acactggcaa
      901 acactctggc ggcaggagtt agatgagatg cgccagcgta ttgcgcagtt gcggcaggat
      961 tttgtcacgg gtatgaatgc gcgcggtgca gcagggcgat ttgattttat attggcacag
     1021 ttcggaatgt tctcatttct cggtttgagt gctaagcagg tacaggaatt gatgtcccgc
     1081 tacagtattt accttttaag caactccaga gctagcattg ctgggcttaa tcgcgctaat
     1141 cttgactatg tgtgtgatgc aataataaaa gttgtttaat gtttctttaa gtgtaagggt
     1201 aagtaagcgg caacttaaga aacaacatta aaaatccatc atgctcacgg ccaatagtgc
     1261 agccctatgg tttaccccca tcctgattgg acgcgatgcg gttggtaaat gtgcttaagg
     1321 gcacctgcac cattggcgtg taatggtttc ttcaggtgcg gtttaatggc gtcgagtctg
     1381 caaaagatgt atgtaagcga tctgcgcgtt ggcatgtatg tggccaaact ggatcgcgac
     1441 tggctggaaa cgccgtttat tctgcagggc tttgtgttgg agtctcaaga agacgtcgac
     1501 atagtggccg agtactgcag ctatgtgtac gttgataccg tttacaaagc cctcgccact
     1561 cctgcggcca gtggtacccg cggcaaaggg cctattcaat atcaaaacac cacccgcatt
     1621 gatgaagagc accgtaaaat cgtcggcgct ttttgccagg cgcgcaccct cacaaagtct
     1681 ttgttggacg atatacgttt aggcggtgcg attaatactg atcacgccaa acaaatggtg
     1741 agcgactgcg tgcaatcggt gatccgccat cccgatgcct taatgtggat ggcaaaaatt
     1801 cgcagtgaaa gtgaatacac cgccgaacac tgtttaaatg tgtgtatttt ggcggtggca
     1861 tttggtcgcg tactcggtat gacggaggaa gagctgcaca agttaggtat gtgtgggctg
     1921 ctgcacgatg tgggcaaaat gaaagtgccg ccggatattt taaataaacc cgcctcactg
     1981 accctcggcg aaatgcgcac tatgatggcc cacactattc acggtcgcaa tttactcttg
     2041 agcaccgatg gaatttacaa cggcgtcatc gacgtcgcct acagccacca tgagcgggta
     2101 gatggcaagg gttacccgcg caaattagct ggcgccagca tctctctttt ctccaaaatt
     2161 attgccatag tggatgccta cgacgccatg acggcggatc gctgttatca atcagcgaaa
     2221 acctccaccg aggcgttaaa aattatttat caggatcgcg gctctcactt tgatgagcgc
     2281 ctagcgctta aattcttaca gaccgtaggc ctgtacccgc ccggtagtat cgtggaatta
     2341 cattcgggcc aggtggcgat tgtggtggaa actaaccata agttgcgcca cttgccgaag
     2401 gttttattgg tgcgggatca aaataaaaag cccttggaaa aagaactttt tatggattta
     2461 tcgcaaattg aagagggcac tttgccgcag gagtatttga ttaaacaggt gtggaaggat
     2521 cgcagctttg gcatttccct gcgcaaatat attgagcagg gcttggtgtt aaatcgctag
     2581 gaagagtact ggagattctt caacggcgtt tttagactgt ttttttactt taaatcctta
     2641 aatcctttcg ggcgccgctg cgcttgtcct gccgctaaat cctcgttgcc ttcaagtgcg
     2701 tgcaacagct ggtcaattgg ctcagcgggg cgcgtaggcg agcaattgct ggtgttgctc
     2761 tgccgccaag cgcggcccgt attggctcac caccaggccc gcggcgcggt tagcaaaggc
     2821 ggccgcctca aagtagttcc agccgcgccc gatgccatgc aaaaacgccc ctgcaaacat
     2881 atcccccgcg ccgttggaat cgagggcttg cacaggctgg ctggggacca gttgcaactg
     2941 ctcaccatca aatacccacg cgcccttggc gccgcaggtg accgcaaagg ttttggctat
     3001 tcctttgagg gcctcaaagg ccgcgtctag atgggtagtg cgcgtgaaat taagtgcttc
     3061 ggcttcgttg caaaacagaa tatccacacc gctgccgatc atggcggtga ggccgtcgtg
     3121 aaaatagctc actatggctg ggtccgacag gctaagcgcc gtgcggacac cgagtttttg
     3181 cgcggcttca cgcagcgtga tcgccgcggg tcggccggat tctaatgtca ctaaatagcc
     3241 ttcaatgtag gcaaaacgcg ccttagcaag ggaatcgaaa tctaaatccg cagtgctgag
     3301 gctggcgctg atacctaaaa aggtgttcat ggtgcgctcg gcgtccgggg taatcatgac
     3361 caggcatttg ccggtaatgc cgtgagcggc gcgggcgcag gccggatagg cgaccttggc
     3421 ccggcttagg tcctgcaggt aaaaatggcc attgtcgtca tcagccactt tgcagctgta
     3481 aaaacagctc gaaccgaatt gggcggcggc gataatggaa ttggcagccg agccgccgct
     3541 ggcgcgtttg gctttgacta ggtggccatc taacaggcgt atcaactcgt gctggcgcgc
     3601 ttcatccacc agggtcataa cacccttagc gatgttaaat tcacgcaggt tgtcatcctg
     3661 caccgtaatt tccgtgtcca ctaaggccgc acccagcccg taaatatcgt aggttttcat
     3721 aattcagctc acatttgcac aggccgcgat tatcggcaat tatgcaggct gcgcctagtg
     3781 cagaagccgc gtcttgacgg ccgggctgat ggcgcttgga ctatgctaga gtgcaccgct
     3841 ggcctgctgt tgggtgcagg ctactaggca taacttaata tgcgctatac cccggatttt
     3901 tatagcgagc gcaactatac tttaaacgcc ttgggccaaa gctatagcgc tgtggcgatg
     3961 tgaggggcgc gatctgcgcg caggtgtgga tcttaaaaaa taaatgaggc ggtatgtcca
     4021 gactaattcg cgacgataaa aataccaagg gcgcagaagc ggtgttaccc gaggtgcaat
     4081 tacccttaaa ccgggaaacc gtgattgcgc atttaaagcg ctgccagcag gtggtaaacg
     4141 aatgcgccag gcaagcgttt aatcgctacg ctgagcgttt ggatgaagcg tttaatatcg
     4201 ccatcgacaa cgcccgctcc aaccaagaag tggccgaaat ggccggtgtg cagcgcctgg
     4261 tgcgcaaaaa ccgcctggaa ttagagcgct attttaccgg ctacatcgcc gagggttttg
     4321 ttaaatttaa aaataaagaa ctgcgcaccg atttaagttc aactaatcgc acctctgaat
     4381 taagcctagt tgataatgaa gatttagatg aaatgatcgt gctgacctcg atcacctcca
     4441 aaatggattc ttattttgcc gagcccattt gggcgctgaa tcaacgtttc gctattttaa
     4501 atggcggcga gcaagtaacc gaagcgggca accctgcagc ccccattcaa ttgtgtgaat
     4561 ccctgcgccg tgcgctgcga ttaattccgc tgaccgctaa agccaaaact atctgttatc
     4621 gcacctatga agagcaccta accggttttg taggctccgt ggtggacgag ctcaacgctt
     4681 attttaaacg ctcgggtatt ttgccgaatt taaaatatat gccgcctgcc agcagccgcg
     4741 gtgcaattaa tatcggcaat accgaatttt caccccagga tttagcgttt atgcagtccc
     4801 tgggctcgcc tgagcaccaa accgaattac tgcaggccat tcgcggctta caggcgagtt
     4861 tgggcggcgt gcagccgctg ccagaagggg tggcgatgct cagcaccgag cagctggtga
     4921 atgtgctgca gagtatccag cttgccacta atctgccgaa tttggcggag gggcaggcat
     4981 tagcgcctgt caatatcgcc caaagcaccc agcacttggc ggaacaggtc aaacagcagg
     5041 gcggtgatgt ctcccaagtg gatatgcaca ccattgattt ggtgggcatg gtatttgagt
     5101 acatgctgag cgatgaaaat ttgccggact cggtgaaagc tctgctgagc tacatgcaca
     5161 cgccattttt aaaattggcc tttagtgacc ccggtttttt tgagcgccca gagcacccgg
     5221 cgcgggtgtt attgaacagc ctggctgagg cgggcacccg ctttgtcggc aatgatggct
     5281 cggtacaata cgacatgtac caaaaaatta aggatgtggt ggatcgcgtt attaaggact
     5341 ttaataatga tgtcaacgtc atcgccgaat tatttattga atttaacagc tatacgaaaa
     5401 atattattcg ccgccaggaa ttaatggaac ggcgcgccac agagcgtgtg cagggcgaag
     5461 agcgcttgcg ggaggtgaaa attcgcgtca acg
//
LOCUS       DAOQGS010000256         5489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7522_length_5489_cov_5.003497, whole genome shotgun sequence.
ACCESSION   DAOQGS010000256 DAOQGS010000000
VERSION     DAOQGS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7522_length_5489_cov_5.003497"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1019)
                     /locus_tag="PKZ52_10435"
     CDS             complement(12..1019)
                     /locus_tag="PKZ52_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNG60231.1"
                     /translation="MKVLITGTAGFIGSSLAQRLLARGDQVIGLDNLNDYYDVNLKKA
                     RLARLTCQSGFTDIRANLEDKTAVNQAFIQHRPDRVVNLAAQAGVRYSLENPQAYIDA
                     NITGFLNILEACRHYGCENLVYASSSSVYGANTAMPFSVHNNVDHPVSLYAASKKANE
                     LMAHTYSHLFNIPTVGLRFFTVYGPWGRPDMALFLFTRKILAGEPIDVFNYGHHRRDF
                     TYIDDIVEGLVRTLDKVATPNAQWNGDSPDPATAKGPYRLYNIGSNRPVELLRYIEIL
                     EGCLGKKATKNLLPLQLGDVPETYANVDALIQDVDYQPSTPIEVGVENFVRWYRDYYQ
                     A"
     gene            1263..2645
                     /locus_tag="PKZ52_10440"
     CDS             1263..2645
                     /locus_tag="PKZ52_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNG60232.1"
                     /translation="MSYIRINKHYIHTPYLWLGVVETGALAGGSLWVQTMLNLPPSLP
                     GASVFALILGCCTLSMGVYGALLRESVLSMVLRVLVSFCLLGAAALFILGQFWTIECF
                     ASNAVFWSVIAAGLIVVPVRMAFRALVDLNQLRRRVVLFGAGMRAQKLLAELAPEKST
                     LAIEVVGCIANKNETVEVDQALILPEPADWLTFAKTEQISEIVICPDERRRSDGSEFP
                     LVALLDCKLAGIESSFALSFYERELGKIDLSSLQPSWMLFSDGFKYSKRSLLAKRILD
                     LLLAGSVFLMVWPLMLLTALAVRLESPGPVLYHQTRVGLNGRHFRIYKFRSMRTDAEK
                     TGAQWAVKNDARITRVGAFIRNTRLDELPQLYNVLSGSMSFVGPRPERPEFVVDLAKQ
                     IPYYELRHKVKPGLMGWAQLKYPYGASVDDARHKLQYDLYYTKNHSFFIDMLIMIQTV
                     EIILLGKGVH"
     gene            2681..3310
                     /locus_tag="PKZ52_10445"
     CDS             2681..3310
                     /locus_tag="PKZ52_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNG60233.1"
                     /translation="MARLGFSFWLRATCLALVFALSGCANQAAQEAPPAAEAAPIGDY
                     KIGVGDSLQVNVWRNPELSLAVVVRPDGKISLPLVGDVAAAGQTSTALATNLATALNN
                     YVRSPQVTVIVTNPVSTDFQRRVRVTGAVHSPQSLPYREGMTVLDLVLLAGGTSDFAS
                     GNNAKLYRKVNGQTKVFSILLEDLLEDGRLETNYLLQPSDIVTVPERAF"
     gene            3390..4907
                     /locus_tag="PKZ52_10450"
     CDS             3390..4907
                     /locus_tag="PKZ52_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length-determining protein"
                     /protein_id="HNG60234.1"
                     /translation="MDKVYLKEMLLALKAELIRFRFLVVFLFVMASFAVLGVGVIWPK
                     HYSTSAILFADETNIIQPLLKGAAEMTQIDRSEQASEIIYTRAVMSAAAKSLGLINDN
                     TPQEQQNQVLRGLRGGLRINKERNNNYFQISYQGANPDQVFETLNAVVNAFIEHTARK
                     KRDESLGAYTFIDTQVQTYKKQLEAAEEKLKEFNARNTDGTESGVQGRLGQLRQDIET
                     LKLTIEETQSRMASVQRQLGSEGQYQQARGQSDDLRARRQTLVAQMEQLRLSYQDSYP
                     DIVSLKQQIKEIDTSLGKLRTSGEVVSTGGKVENPLYEDLRKQLANTEVDLRTQTRRL
                     ESLIALQEEEKLRAERVAANQAQLSELTRDYDVTKRVYEEMLGKKETARLSMTLDIEG
                     QGVSYRIQEAAQFPLRPTGLNFKHFAALGPIIGFLLPVGLLIAYVVVDPHLRSSRILQ
                     KQLPPDIELIGAIPHYDTPLGQRLLQKDMIFLLVVVVLTLAAYVATAIYWHINHS"
     gene            4918..>5489
                     /locus_tag="PKZ52_10455"
     CDS             4918..>5489
                     /locus_tag="PKZ52_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60235.1"
                     /translation="MDQHPEGHLAADALENSEQLLKAALDDNRRRRAIKNMALPIMWT
                     QEDLYEKKILFTGMRQRRFLNAFREIRIRLLQKCKQDNVVLLVSSVVPRGGSSFFAFN
                     LAATFALDQHKTALYVDCNPYSASSERYCATPVEQGLMHYLTDYTVPIENIIYPSGIE
                     RLRVIPSGGSSESANSRSTRRRPATSSSRA"
BASE COUNT         1302 a         1334 c         1485 g         1368 t
ORIGIN      
        1 aggcagtaaa tttaagcctg gtaatagtcc cgataccagc gcacaaaatt ttctacgccc
       61 acctcaatag gggtgctggg ttgataatcc acatcctgta tcagcgcatc cacattggca
      121 taggtctcag gcacgtctcc cagttgcaga ggcagcaggt tcttggtggc ttttttgccc
      181 aggcagcctt ccaaaatctc gatataccgc agcagctcca ccggccggtt gctgccgatg
      241 ttgtagagcc ggtaagggcc tttggcggtg gctgggtctg ggctgtcgcc gttccactgg
      301 gcgttaggtg ttgcgacctt atcaagggtg cgcaccaggc cctccactat atcgtctata
      361 taggtgaaat cccggcggtg gtggccgtag ttaaaaacgt cgatgggttc gcccgctaaa
      421 attttgcggg tgaataaaaa cagcgccata tccgggcgcc cccaggggcc ataaacggtg
      481 aaaaagcgca ggccgacggt ggggatgtta aataaatggc tgtaggtgtg ggccattaac
      541 tcgttggctt ttttgctggc ggcgtacagg ctcaccggat ggtccacatt gttgtgaacg
      601 gaaaagggca tggcggtgtt ggcgccatac acggagctgc tggaggcata cacgaggttt
      661 tcacagccgt agtggcggca ggcctccaaa atattcaaaa aacctgtgat gtttgcgtcg
      721 atataggcct gcgggttttc caaggagtag cgcacccccg cttgggctgc gaggttaact
      781 actcggtccg ggcggtgttg gataaacgcc tggttaacag ctgttttgtc ttctaaatta
      841 gcgcggatgt cggtgaaacc gctctggcaa gtcaggcggg ccaggcgggc ttttttcagg
      901 ttaacatcat aataatcgtt gagattatcc agcccgatca cctggtcccc gcgcgccaaa
      961 agccgctgcg ctaaggatga gccaataaac cccgctgtgc cggtaatgag tactttcata
     1021 acgcgtgcct aataagaatg aataagaact agtctagcgg gtttacttgg caggggaaaa
     1081 catgcgctac tgttcggcct gtgggcgtga aaatatcacc gcatttaacc tcttggccga
     1141 ttaggcttga gctgccttga gcgcagctcg tgaaggcgta agaattcccg ctataatggc
     1201 gaattgcggc gccaacatca gccattagtc attgcccttt agcctgtttg tgggaagtca
     1261 ttttgtctta tatccgaatt aataagcact atatccacac cccatacctg tggctgggtg
     1321 tggtcgaaac aggcgcctta gcagggggca gcctgtgggt gcagacgatg ttaaacctac
     1381 cgccctcatt gccgggggcc agtgtgtttg cgctgattct cggctgttgc accctctcca
     1441 tgggtgtgta cggcgccctg ctgcgggaga gtgtgctgag catggtgctg cgggtgctgg
     1501 tgagtttttg cctgctcggc gctgccgcgc tgtttatttt ggggcagttt tggaccatag
     1561 agtgttttgc ctccaatgct gttttttgga gcgtgattgc ggcggggtta attgtggtgc
     1621 cggtgcgcat ggcatttcgc gccctggtgg atctaaatca attgcggcgg cgcgtggtat
     1681 tatttggcgc cggtatgcgg gcgcaaaaac tcctcgccga attagccccc gaaaaaagca
     1741 ccttggcgat tgaagtagtt ggctgtatcg ctaataaaaa tgaaacggtg gaagtggatc
     1801 aggcgttaat tttacccgag cctgctgact ggttaacatt tgccaaaact gaacaaatct
     1861 ctgaaattgt catttgtcct gacgagcgcc gccgcagcga cggcagtgaa tttcccttgg
     1921 tggcactgct tgattgtaaa ctggccggca tcgaatcctc cttcgcgctg agtttttatg
     1981 agcgcgaatt gggcaaaata gacttatcca gcctgcagcc gagctggatg ttattctccg
     2041 acgggtttaa atattccaaa cgctctttat tggccaagcg catattggat ttgttattgg
     2101 cgggcagcgt atttttaatg gtatggcccc tgatgctgct caccgctttg gcggtgcgct
     2161 tggaaagccc aggcccggtt ttgtaccacc aaacccgcgt tggcttgaat gggcggcatt
     2221 ttcgcattta taaattccgc agtatgcgta ccgacgcgga aaaaaccggt gcccagtggg
     2281 cggtgaaaaa cgatgcgcgc attacccgcg tgggggcgtt tattcgcaat acccgtttgg
     2341 atgaattacc tcagttatac aatgtgttat cgggcagtat gagttttgtt ggcccgcgcc
     2401 ctgagcggcc ggaatttgtg gtggatttag ccaaacaaat tccctattac gaattgcgcc
     2461 acaaagtaaa acccggcttg atgggctggg cacaattaaa atacccctat ggcgcctccg
     2521 ttgacgatgc gcggcataaa ttgcaatacg atctttatta caccaagaac cacagctttt
     2581 ttatcgatat gttaattatg attcaaactg tggaaattat tttgctcggc aagggtgtgc
     2641 actaaacacc tgcactgttt taacttaaga ggaattaatc atggcacgtt taggtttttc
     2701 attttggttg cgcgccacct gcttggcgct ggtttttgcc ctgagcggtt gcgccaacca
     2761 ggctgcgcaa gaggcgccac cggcagccga agctgccccc atcggcgact ataaaattgg
     2821 tgtaggcgat tctttgcaag tgaatgtgtg gcgcaaccca gagttatccc tggcggttgt
     2881 ggtgcggcca gacggcaaaa tttcgctgcc actggtgggc gatgtggcgg ctgcggggca
     2941 aacctccaca gccttagcga cgaatttggc cacagcatta aataattatg tgcgcagccc
     3001 ccaggtaact gtgatagtta ccaacccggt cagcacagat ttccagcgcc gcgtgcgggt
     3061 caccggtgcg gtgcatagcc ctcaatcgct cccctatcgt gagggcatga cggtattgga
     3121 tctggtattg ctcgccggcg gcaccagtga ttttgcctca ggcaataacg ccaagctcta
     3181 tcgcaaggtt aacggccaaa ccaaggtgtt ttctattttg ttagaagacc tgctcgaaga
     3241 tggccgcctg gaaactaatt atttattgca gccctccgac atagtcaccg tacctgagcg
     3301 cgcgttttaa ttttcgatcg atagtgcgct ttatctagcg cgtagaacac ctctacgcgc
     3361 tagtcatttt ccctatagcg agcaagcgga tggataaggt ttatctcaaa gagatgctgt
     3421 tggccttaaa agcggagtta attcgctttc ggttcttggt ggtattttta tttgttatgg
     3481 caagttttgc ggtattgggt gtgggcgtca tttggcctaa acactacagc acttcagcca
     3541 tattgtttgc cgatgaaacc aatattattc agccgctgtt aaaaggcgcc gctgaaatga
     3601 cgcaaattga tcgctcggag caggccagtg aaattatcta tacccgtgca gtgatgtcgg
     3661 ctgcggccaa atccctcggg ctgattaatg acaatacgcc ccaagagcag caaaatcagg
     3721 tattgcgcgg cttgcgcggc ggcctgcgga ttaataaaga gcgcaataat aactattttc
     3781 aaatttccta ccagggtgcc aacccagatc aagtatttga aaccctcaac gctgtagtaa
     3841 atgcctttat agagcacaca gcgcgcaaga aacgcgatga aagtttgggt gcttatacat
     3901 ttatcgatac tcaagtgcag acctataaaa aacaattgga agcggcggaa gagaaactca
     3961 aagaatttaa cgcccgcaat accgatggta ctgaaagcgg cgtgcaaggg cgcttggggc
     4021 aattgcggca ggatatagaa actctgaaac tcaccattga agaaacccag tcgcgcatgg
     4081 cgagcgtgca gcgccagttg ggcagtgagg gacaatatca acaggcgcgt gggcaatcgg
     4141 atgatttgcg cgcgcgcagg caaaccctag tggcgcaaat ggagcaattg cgtttgagct
     4201 atcaagacag ttaccccgat atagtttccc ttaaacagca aattaaagaa attgacacct
     4261 ccctaggtaa gttgcgcacc agtggtgaag tcgtgagtac cggcggtaaa gtggaaaacc
     4321 cactctatga ggatctgcgc aaacaattgg ccaataccga agtggatctt cgcacccaaa
     4381 cccgccgtct cgaatcatta atcgccttgc aggaggaaga aaaattgcgg gctgagcgtg
     4441 tggcggccaa ccaggcgcaa ttatccgaat tgacgcggga ttatgatgta accaaacgtg
     4501 tgtacgagga aatgctcggc aaaaaagaaa ccgcgcgcct atccatgact ttagatattg
     4561 aagggcaggg tgtgagctac cgcatccaag aggcggcgca atttccgctg cgccccacgg
     4621 ggttgaattt caaacatttt gccgcgctgg ggccaattat tggtttttta ttgccggtgg
     4681 gtttgttaat tgcctatgtg gtagttgacc cccacttgcg ctcatcgcgc attttacaaa
     4741 aacaactgcc gccggatatc gaactcatag gtgccatccc ccactacgac accccgctgg
     4801 ggcaacgcct gctgcaaaaa gacatgattt ttcttttggt agtagtggtg ctgaccttgg
     4861 cggcttatgt ggcaaccgca atttactggc atattaatca cagctaaggg ggaaacaatg
     4921 gatcagcacc cagaggggca cttggcagca gatgccttag aaaacagcga gcaattatta
     4981 aaagctgcgc tcgatgacaa tcgcaggcgc cgcgccatta aaaacatggc gctgcccatt
     5041 atgtggacgc aagaggattt gtacgagaaa aaaattctct tcaccggcat gcgccagcgc
     5101 cgatttttaa atgcgtttcg cgaaatccgt atacgcctgc tgcaaaagtg caagcaagat
     5161 aatgttgtgt tgctggtaag ttccgttgtg ccgcgcggcg gcagcagttt ttttgcattt
     5221 aatttggcgg caacttttgc attggatcag cataaaaccg cgctttatgt tgattgtaat
     5281 ccttacagtg cttcatctga gcgctactgc gcaacgccgg ttgaacaggg gttaatgcat
     5341 tatctaaccg actacaccgt gcccattgaa aacatcattt acccttcagg tattgaacgc
     5401 ttgcgcgtta tcccctcggg cggctccagt gaatcggcga attccagatc aaccagacga
     5461 cggccggcaa ccagcagttc ccgagcgtg
//
LOCUS       DAOQGS010000257         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7586_length_5459_cov_4.558105, whole genome shotgun sequence.
ACCESSION   DAOQGS010000257 DAOQGS010000000
VERSION     DAOQGS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7586_length_5459_cov_4.558105"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKZ52_10460"
     CDS             complement(<1..603)
                     /locus_tag="PKZ52_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNG60236.1"
                     /translation="MPTNALLRRLPADANCPSADLLDRFLDYITELNLELYPAQEEAI
                     LELFEGNNVILTTPTGSGKSLVATALHFKAMAQGRRSVYTCPIKALVNEKFLALCRDF
                     GPENVGIATGDATVNRDAPIICCTAEILANVALSEGAEAPFTEIIMDEFHYYSDRSRG
                     VAWQLPLLTLNRARFLLISATLGAIDQFMLMWAGDTVPTNA"
     gene            986..2602
                     /locus_tag="PKZ52_10465"
     CDS             986..2602
                     /locus_tag="PKZ52_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNG60237.1"
                     /translation="MTLNYRPSRPLIKKALNLSLVIGALSAAAAWAENPIISQTYTAD
                     PAGRVFNDRMYVITSHDPDNATDYSTLLDYNLISSDDMVNWQDHGIVFAARKNTTWAG
                     LAYAPDWVKKDNKYYLFFPDGASAIGVAVADKPEGPYTDHIGKALITKSTPNSNVEWL
                     FDPGVFVDDDGRTYVYFGGGGPGNARVIEVNSDYKSVKGSAVTIDAPNFFEASYMHKR
                     NGVYYFSYSTNGNKSFAIDYMTSSNPMTGFQYRGSVLPNPWENNGNNNHHSIVEWKGQ
                     WYMFYHNRAVANANGKGVYQRSVNVDLLSYNSDGTMKVSAGRTGAPQIKNFNPYAQIQ
                     AETMAKQSGIDVENCSEGTLDVTFNANGWVKIAKVDFGAGASSIELRVAATANTSLEI
                     ALDNSASTAIATVPVNSTGGLQTWATQKANVSGLTGVHDVYLRAKGRFNLNWYKFNAI
                     EQNSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGRSSSSSSSSSSSSSSSSSSSG
                     GTVAGALGGNLMWILAGVLAFMRRRPTEGK"
     gene            3125..5362
                     /locus_tag="PKZ52_10470"
     CDS             3125..5362
                     /locus_tag="PKZ52_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HNG60238.1"
                     /translation="MINANPLLRSLLITCCLASAPDVLAKENTDAAKPWLNKKLAATE
                     RANLLIAQMSQEEKFTILYGYFGLEQKEKNYQPPKEALYGSAGWIAGIPRLGIPAQFQ
                     TDASLGVATQNIPKPRERTGLPSGLATAASFDPNVALQGGTMIGQEARASGFNVMLAG
                     GVNLVREPRNGRNFEYAGEDPLLAGKMVGAQIKGIQSNQIISTLKHFALNNQETGRNI
                     GNVIIDESAARTSELMAFQIALKDGNPGSVMCAYNKVNGHYSCENDFLLNKVLKGDWG
                     FEGYVMSDWGAVHSTIPAVNNGLDQESAYGFDRAFYFGPALKEAVDNGWVAPSRLDDM
                     VRRVLRSMFANGLFDLPLKETPIDFAAHAKITQQTAEAGMVLLKNANSILPINKSAKK
                     IAVIGGQADSAVLSGGGSSQVYGQGGNPIKTPQTAMFPKVYHPSSPLQALRALLPGSQ
                     IEFNSGVDLAAAQALAQSADITLVFATQWTSEAYDVDSLNLADGQNELISAIAKANPK
                     TIVVLETGGPVLMPWLNQTAAVLQAWYPGTEGGKAIARVLTGEVNPSGHLPITYPADS
                     NQLPQKTIAGSGRKNDEVPFDIHYPEGATIGYKWYDQQKLTPLFPFGFGLSYSQFTYS
                     KLTAEWNNGQLSVAFNLANNSKTAGKGLGLVFAQPVATIWEAPKRLVGFAKADLKAGA
                     NKKLQLSIDPLMLAVYQPQSKTWQIVEGDYDIRLAQHAMDNDAQVVRVHLAKQTLDIK
                     GNLIR"
BASE COUNT         1422 a         1405 c         1380 g         1252 t
ORIGIN      
        1 ggcattggta ggtacggtat cccctgccca catcaacatg aattggtcga tggcgccgag
       61 ggtggcggaa attaataaaa aacgtgcgcg gttaagggtg agcagcggca actgccaggc
      121 cacgccgcgg gagcggtcgg aataatagtg aaattcatcc atgataattt ccgtaaaagg
      181 cgcctcggcc ccttccgata gcgccacatt ggcaagaatt tccgccgtgc aacagataat
      241 cggcgcatcg cgattcaccg tggcatcccc ggtggcaatg ccgacgtttt ccggcccaaa
      301 atcccggcac agggcgagga atttttcatt gaccaaggct ttaatcgggc aggtgtacac
      361 gctgcggcgg ccctgggcca tggctttaaa atgcagtgcc gttgccacta aggatttacc
      421 cgaacccgta ggcgtggtga ggatgacatt attgccctca aacagctcca aaatcgcctc
      481 ttcctgggcg gggtagagtt ctaaattcag ctcagtgatg tagtccaaaa agcgatccag
      541 cagatcggcg ctggggcagt tggcatcggc gggcaggcgg cggagcaggg cgttagtggg
      601 catgggatgg gcaggtagcg gtggctttta agggcggcca gcatagcagc cccggtgcgc
      661 tctggcgccg ggcaatttat gcactgtccc acctatttac agcgggcgaa tccggcactt
      721 atgcaatcgt ctgacaaaaa tgcgacggcc ttcatggtca atgctgcttc agtgtcgatt
      781 ggttcaccat gactattaga ggctgcgggg ataattgtct taccaattaa cgcgctcgga
      841 tgaaaaaccg aaccgcctag cagtggccgc ttagagccct atcgctgcac atccgggctg
      901 cgcgaataat tgttccacca cctactaata attcccccgc gccgcatatg ggggccagac
      961 cataataatt acctgggagt acaccatgac cctaaactat cggccgagcc gccccttaat
     1021 taaaaaagcc cttaatttaa gccttgtgat aggcgccctc agcgcagcgg cggcctgggc
     1081 ggaaaacccc ataatttccc aaacctacac ggcggatcca gctggccgag tgtttaacga
     1141 tcgcatgtac gtgatcacca gccacgaccc agacaacgcc accgactaca gcaccctgtt
     1201 ggattacaac ctgatttcca gcgacgacat ggtgaactgg caggaccacg gcattgtgtt
     1261 tgccgcgcgc aaaaatacca cctgggcggg ccttgcctac gcgccggact gggtgaaaaa
     1321 agacaacaaa tattatttgt ttttccccga tggcgcctct gccatcggcg ttgccgtagc
     1381 ggataaaccc gaaggcccct acaccgatca cataggcaaa gcgctgatta ctaaaagcac
     1441 ccctaacagc aacgtggaat ggttattcga cccaggtgta tttgtggatg acgacggccg
     1501 cacctatgtg tatttcggcg gtggtggccc cggcaatgcg cgggttattg aagtgaatag
     1561 cgattataaa agtgtaaaag gttctgctgt taccatcgat gcgccgaatt tttttgaagc
     1621 ctcctacatg cataaacgca atggcgttta ttattttagt tattccacca acggcaataa
     1681 aagtttcgcc atcgattata tgaccagcag taaccccatg acgggctttc aatatcgcgg
     1741 cagcgtattg cccaacccgt gggaaaataa tggcaataat aatcaccatt caattgttga
     1801 gtggaaaggc caatggtata tgttttacca caaccgcgcc gtggctaatg ccaatggcaa
     1861 aggcgtttat cagcgctctg ttaatgtgga tttattaagt tacaactcag atggcaccat
     1921 gaaggtttcc gctggccgca ccggggcgcc gcaaattaaa aactttaacc cctacgcaca
     1981 aatacaagcg gaaaccatgg ctaaacaatc gggtattgat gttgaaaact gcagcgaagg
     2041 caccttggat gtcaccttta atgccaatgg ttgggtaaaa atagccaagg tggattttgg
     2101 tgcgggcgcc agcagtattg aattgcgcgt ggcggcaacg gctaacacca gtttagaaat
     2161 tgccctggat aattccgcca gcacagctat tgccacagtg cctgttaact caaccggcgg
     2221 cctgcaaacc tgggccacgc aaaaagccaa tgtgagcggt ttaaccggcg tgcacgatgt
     2281 gtatttgcgg gctaaaggcc gttttaattt aaactggtat aaattcaacg ccatcgagca
     2341 aaacagcagt tccagctcat cgagcagttc gtcttcttcc agtagcagct catcttcatc
     2401 ttcttcatcc agttcgtcct ctagttcctc ctcaagctcg tcgggcagat catccagttc
     2461 gtcttccagc tcttcgtcta gctcttctag cagctcaagc agcagcggcg gcacagttgc
     2521 aggcgcattg ggcggtaatt taatgtggat cttggcgggt gtattggcat ttatgcgccg
     2581 ccgcccaacg gaaggaaaat aaacgcggtt taaaccttct atttaattta ttgttggcca
     2641 aacctgaacc gccgctacat gggtagcggc ggtttttttg tgcgcagaaa taaaagggga
     2701 actctgagta ccgcctgtat ggtgttttac tttgtaggac gtgtaataaa gccaagcgca
     2761 tcgtcccatc ccaaatttaa cgccgccaga gctagcaagg tagtatttca acaagctgtt
     2821 aaaggactgt gcaaaattct tagtttaata atatcgctct tgggtgtggc ctagatccct
     2881 gcgcctaaaa cccagcatac atatctgact taattgaggc gcttttgtcc cgctgcgtat
     2941 agtggtgggc cacaaagctt tgccctccat caactttaaa cacccgccct gaggccttaa
     3001 gcgctttata tttgctacca taagcactgg gcagggcggc gctttacacg ctaaaaccgc
     3061 accctaaacc tgcgcacctt atcaccagta taagggccat aataacgata agctgagaac
     3121 cgtcatgatt aatgcaaacc cattactgcg cagcctgcta atcacctgtt gcctggcaag
     3181 cgcaccggac gttttagcca aggaaaatac tgacgcggcc aaaccctggc tgaataaaaa
     3241 attagcggcc accgagcggg ctaatttatt aattgcccag atgagccagg aggaaaaatt
     3301 cactattttg tacggctact ttggcctgga acaaaaagaa aaaaattatc agccccctaa
     3361 agaagcgctc tatggttccg ccgggtggat tgcaggtatt ccccgcttag gcattcccgc
     3421 acaatttcaa accgatgcca gccttggggt tgccacccaa aatattccca aaccccgcga
     3481 gcgcacgggc ctgccctcgg gccttgccac cgccgccagt tttgatccaa acgtcgctct
     3541 gcaaggcggc actatgatcg gccaggaggc cagggcgtca ggctttaatg tgatgctagc
     3601 aggcggtgta aacctggtgc gtgagccgcg caatggccgc aattttgaat acgccgggga
     3661 agatcctctg ctggccggta aaatggtggg tgcgcaaata aaaggcattc aaagcaatca
     3721 aattatttcc accctgaaac attttgcgct caataaccaa gaaactggcc gcaatatcgg
     3781 caacgtcatt atcgatgaaa gcgcggcgcg cacctcggaa ttaatggcat ttcaaatcgc
     3841 tttaaaagac ggcaacccag gctcagtaat gtgcgcctat aacaaggtga acggccatta
     3901 cagttgcgaa aatgattttc tgttaaataa ggtattaaaa ggcgactggg gttttgaagg
     3961 ttatgttatg tccgactggg gcgctgtgca ctctaccatt cctgcggtaa ataacggctt
     4021 ggatcaagaa tccgcctacg ggtttgatag ggcattttat tttggcccag cattaaaaga
     4081 agcggtggat aacggctggg ttgctccttc gcgtttggat gatatggtgc ggcgggtgct
     4141 gcgcagtatg tttgccaatg gtttgtttga tttgccctta aaagaaaccc ccatcgattt
     4201 tgccgcccac gccaaaataa cccagcaaac cgccgaggcg ggaatggtgt tattaaaaaa
     4261 tgccaacagc attttgccca ttaataagag cgcgaaaaaa atagccgtga ttggcggcca
     4321 ggcggatagc gccgtgctct ccggcggcgg ctcctcccaa gtctacggcc agggtggcaa
     4381 ccccattaaa acgccgcaaa cagctatgtt ccccaaggtg tatcacccgt cttcgcccct
     4441 gcaggccctg cgcgcattat tgcctggcag ccaaatagaa tttaattccg gcgtagatct
     4501 ggccgcagcg caagccttag cccagtcggc ggacataacg ttggtatttg ccacccagtg
     4561 gacatcagaa gcctatgatg tagattcgct aaatttagcg gacgggcaaa atgaattaat
     4621 ttccgcaatc gccaaagcca accccaaaac cattgtggtg ctggaaaccg gcgggccagt
     4681 attgatgccg tggcttaatc aaaccgctgc agttttacag gcctggtacc caggcaccga
     4741 aggcggcaaa gccattgcgc gggtattaac cggggaggta aatccctccg gccatttacc
     4801 tattacctat cctgctgata gcaaccaatt gccgcaaaaa actatcgccg gtagcgggcg
     4861 taaaaatgat gaggtgccgt ttgatattca ctaccctgaa ggcgccacca ttggttataa
     4921 atggtacgac caacaaaaat taacgcccct attccccttt ggttttggcc tgagttacag
     4981 ccaatttacc tattcaaaat taaccgccga atggaataac ggccaattaa gcgtggcatt
     5041 taatctggcc aacaacagca aaacagcagg caaaggcttg gggttagtat ttgcacaacc
     5101 ggtagcaact atctgggaag cgccaaaacg cttagtgggt tttgccaagg cggatttaaa
     5161 agccggcgct aataaaaaac tgcaactgag cattgaccca ttaatgctcg ccgtttacca
     5221 accccaaagc aaaacatggc aaattgtcga gggcgactat gatattcggc tggcccaaca
     5281 cgccatggat aatgacgccc aggtggtgcg ggtgcatctt gctaagcaga cattggatat
     5341 aaaaggcaat ttaattcgct aacccatata aatggaattg ttgcgcagca gggaagctgt
     5401 gtggggcctg ggatagaagt aatatctaca gcacggcggg tgggttaaca gcgtgctgc
//
LOCUS       DAOQGS010000258         5457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7587_length_5457_cov_4.463902, whole genome shotgun sequence.
ACCESSION   DAOQGS010000258 DAOQGS010000000
VERSION     DAOQGS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7587_length_5457_cov_4.463902"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKZ52_10475"
     CDS             complement(<1..383)
                     /locus_tag="PKZ52_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNG60239.1"
                     /translation="MLGKIVSNTPNTGRAGANFVGIIGVGILIIALGMQSFYTVSEGY
                     RAVILRNGSLVGTAEPGFGLKIPFMDSVVKISVRDNIARLELPTYSFDQQLATSRISI
                     SYRIMADKVDEAFSRYGGEQGVVER"
     gene            complement(700..1176)
                     /locus_tag="PKZ52_10480"
     CDS             complement(700..1176)
                     /locus_tag="PKZ52_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNG60240.1"
                     /translation="MEQTPPPPAVYPYALMLLSRREYSKAELKQKLLRRFGADAKALI
                     EATLERLAQDDYQSDQRFTESFINSRLHRGYGLNRIKLELRQKGIDQEGISHMIKDEN
                     IQAKEQQQLERMWRKKFNTQPKNSQERYQQQCHLANKGFRKELIEQFFRSSTWDDE"
     gene            complement(2180..3220)
                     /gene="recA"
                     /locus_tag="PKZ52_10485"
     CDS             complement(2180..3220)
                     /gene="recA"
                     /locus_tag="PKZ52_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNG60241.1"
                     /translation="MDANKEKALQAALSQIDRQFGKGTVMRMGDRERVNIPAISTGSL
                     GLDVALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAEAQRKGGTCAFIDAEHALDPIY
                     AEKLGVNVDELIVSQPDNGEQALEVADMLVRSGAVDVLVVDSVAALTPKAEIEGEMGD
                     HHVGLQARLMSQALRKITGNIKNANCLCIFINQIRMKIGVMFGNPETTTGGNALKFYA
                     SVRLDIRRTGSVKEGEEVTGSETRVKVVKNKVAPPFKQAEFQILYGQGINRLGEVVDY
                     GVKLGMIDKAGAWYSCQGEKIGQGKQNAIQYLREHPELAQSLEERIKTEMLGSGAGLT
                     TASAEDFAEEEM"
     gene            complement(3377..4093)
                     /locus_tag="PKZ52_10490"
     CDS             complement(3377..4093)
                     /locus_tag="PKZ52_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNG60242.1"
                     /translation="MQQAIIGYHQDDENHWVAQLACGHNQHVRHNPPWVLRPWVTHIE
                     GRDEHLGLLLNCVKCDQQAPSDERPAPLNPFTTLFPANTLALARVLGRLLQANQAAVS
                     CAESCTGGLIAAAITEIPGSSEWFTHSWVTYSNAAKQQQLGVSSDALAHGAVSQATVE
                     AMAQGAQANSSALCALATSGIAGPGGGTAQKPVGTVWIAWSGPWGINSQCFYFSGDRH
                     QVRLQAVNAALGQLIALLKA"
     gene            complement(4095..>5457)
                     /gene="prsK"
                     /locus_tag="PKZ52_10495"
     CDS             complement(4095..>5457)
                     /gene="prsK"
                     /locus_tag="PKZ52_10495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNG60243.1"
                     /translation="AISFAGYYMSLYGGDWGTIIYSLLLVGTLILLASAFSSSRLREQ
                     LSVLVNKHLFSHKYDYRTEWLNVIDQLSKPSNSEEVYQRALSSVTNIFRSAAGGLWLK
                     RGNVFVPVYQAPDLIAPRQAQEPSDSPFIRALEREEWVFFPQAGSASQNQTPNEQLPN
                     WAKDMTNLWLILPLIDKAELIGFMVLIAPAQNQEALNWEDLDLLKILGRQVAHYLSRQ
                     AQADQLAEARQFETFNRLSAYMMHDLKNLIAQQSLVVKNAAKHKDNPAFVEDAIQTIS
                     NSVNRMQHLLQKLQHTSGEEVRVLLLSEVLMDAVKRCQKAMPAPTLRIDDGDLQVKAD
                     RESLTMVFAHMIQNAQDATLSSGYVDLFITRQDGFAVVLIEDNGQGMDEEFIQNRLFR
                     PFDTTKTGKGMGIGMYQARDCITNLGGEISVESVPGEGTTFFIRLPLVQEGAVALPKP
                     AAA"
BASE COUNT         1128 a         1357 c         1532 g         1440 t
ORIGIN      
        1 aagcgctcga caacaccctg ttcgccgccg taccggctga aggcttcatc cactttatcg
       61 gccataattc ggtacgagat agatatgcgc gaggtggcta gttgttggtc gaaactgtag
      121 gtgggcaatt ccagccgcgc gatattgtct cgtacactga ttttaacgac agaatccata
      181 aacggaattt taaggccaaa gcccggctcg gctgtgccca cgagggagcc attgcgcaga
      241 atgacggctc tatagccttc agataccgta taaaaacttt gcatacccag cgcaataatt
      301 aaaatcccca cgccgataat gcccacaaaa tttgcgcctg cacggccagt gttaggagtg
      361 ttggaaacaa tttttcctag catggttttg atcctcaggg tttgatgtgt gggttaaata
      421 tacacgcagc ggttgatcca tgggcgtgtg gcagcgcaat tttgctgata aatctctctg
      481 tgccgctgtt gtgccgctca ggttaaccac cttgctagat cgttgcggcg gcggggaagc
      541 tgcccaatgc ccgatacatg atcagtgacc gttacggggg gcattaatgt tctgtggaga
      601 ttcggaacaa tgcccggaat actgattttt caagacacac gagtgccgcc atgtagattt
      661 ggctcatgga aaaaatagct gctttactgt gtgattttgc tactcgtcat cccacgtaga
      721 ggagcggaaa aattgctcaa taagctcctt gcgaaagcct ttgttagcta agtgacactg
      781 ttgctgatag cgctcttggc tgtttttcgg ttgtgtatta aattttttgc gccacatgcg
      841 ctccagctgc tgctgttctt tcgcttgaat attctcgtct ttaatcatgt gactaatccc
      901 ctcctggtcg atgccttttt ggcgcagttc caatttaatg cggtttaagc catatcctcg
      961 atgcagacgg ctattgataa aactctcggt aaaacgctga tcggactgat agtcatcctg
     1021 tgccaggcgc tctaaggttg cctcaatcaa cgctttagca tcagcgccga aacgccgcaa
     1081 taatttctgt ttaagctctg cctttgaata ttcccgccgg gataaaagca ttagggcgta
     1141 ggggtaaacg gctgggggag ggggtgtttg ttccattgat gagcctatgg tttggggcga
     1201 tcaaggtgtg taatcgaacg cgcctgttta cttcagcgcg ttttttaatg ccgtgtgctg
     1261 tatatgaatt actgctaggt cctgtgtttt cttgaagctt cacgctttct tcgagagtgg
     1321 gctctatatt atggctatct ctggaaatcc acttttcgcg tccgcggcgc tatttttgtg
     1381 cccagtcaaa tgccttgcgg tcatcacgaa aattgaactt ctattttcac tgggcgtgat
     1441 tctcataccg actagcaacc acccggcgag tgctttagcc gcttgttgaa ctatgtaagc
     1501 ggcgacgctc tggcaaggca aacttggcga aaaaactgga cgattacagt gattgcaaga
     1561 ggtagcgcaa tacaggaaca attgccgagt gtacacgcag taaatgaggt tgttccgcca
     1621 tattaacgcc gtcagagcta gcaaggtagt aattcaacta gctgctattg ctgcgatccc
     1681 tcaatactcc gcccgcagag ataaatgcag gttatttcag accgtcaaaa aaatccctct
     1741 ttttggcggt ctgaaataac ctgtatttac caccgctgca ccgcgatcga tactgccgct
     1801 ttgctatatt tgccacccgg tggagcggtt aatatggcct gccaaatagt tactcatttt
     1861 ttaagatgct tgtttgcatc cgtcagcata ataacgaccg ctggttaaat gttaattctg
     1921 agagctttta gccgtaaaag tatttcatgc tcgattttgt cggtttgggc cgaagtattt
     1981 tcgatgtcac tggatggtaa aaattagtcg tcctttaaaa acgctttgac cgttccttat
     2041 tctcaacctt cttgcgctag agcctagcgg gtttccctgc ccgcgagggt tgcaaaatat
     2101 ttacgtctgt ggacctaaaa gtctcgccag gcaaaacatg ttttcgcgag ccgtttgtaa
     2161 tgtacgtgta attgaaaaat tacatttctt cttcggcaaa atcttcagcg ctggcggtgg
     2221 ttaatccggc gccgctgccg agcatttcag ttttaatgcg ctcctccaag ctttgcgcaa
     2281 gctccgggtg ctcgcgcaaa tattggatag cgttttgttt gccttggcca attttttcac
     2341 cctggcagct ataccaggcg ccggccttgt cgatcatgcc gagttttacg ccgtaatcga
     2401 ccacttcacc taagcggtta ataccttggc cgtagagaat ttgaaattcc gcttgtttaa
     2461 acggcggggc cactttgttt ttgaccactt ttacgcgggt ttccgagccg gtgacctctt
     2521 caccttcttt tactgagccg gtgcggcgaa tatccaagcg cacagaggcg tagaatttta
     2581 atgcgttacc gccggtagtg gtttcggggt tgccgaacat gacgccgatt ttcatgcgga
     2641 tttggttaat gaaaatacac aggcagttgg cgtttttaat attgccggta attttgcgca
     2701 gggcttggga cattaggcgc gcttgtaaac caacgtggtg atcgcccatt tcgccttcaa
     2761 tttcagcctt gggggttagg gcggctaccg agtccaccac cagcacatca acggcgccgg
     2821 agcgcaccag catgtcggcc acttctaagg cctgctcgcc gttatctggc tgggagacaa
     2881 tcagttcatc cacattcacg ccgagttttt cggcgtatat agggtcgagg gcgtgttcgg
     2941 cgtcgataaa ggcgcaggtg ccgcctttgc gttgggcttc ggcaataacc tgtagggtga
     3001 gggtggtttt accggaggat tccgggccgt agatttccac aatgcgcccc ttgggcaggc
     3061 cgccgatgcc gagggcgaca tccaagccca gagagccggt ggaaatagcg gggatgttca
     3121 ctcgctcgcg atcccccata cgcatcacgg tacctttgcc gaattgacgg tcgatttgtg
     3181 acagggcagc ttgtagggct ttttctttat tggcgtccat aaaatcctcg gtaggcggcg
     3241 tattgatggg gtggtgcgcg atggcttctg tgcgagccat ccggcttgat agttttaatt
     3301 tattataaaa atactgtatg cgcaaccagt tagtgtaaat ttatacagtt atttttgacg
     3361 gccaagaacg cggggcttag gcctttaaaa gtgcgatcag ttggcctagg gcggcattta
     3421 ccgcctgcag ccgcacttgg tgccggtcac cgctgaaata aaaacattgg ctgttgattc
     3481 cccagggccc gctccaggca atccacactg tgccgacggg tttttgtgct gtgccgccac
     3541 ctggccctgc gatgccgctg gtggccaagg cgcagagggc gctgctgttg gcttgcgcac
     3601 cttgtgccat ggcctctacg gtggcttggc ttacggcgcc atgggcgagg gcatcgctgc
     3661 tgacgcccag ttgctgttgt ttggcggcgt tggagtaggt gacccagctg tgggtaaacc
     3721 actcagagct gccgggaatc tcggtaatgg cggcggcgat taagccgcca gtgcaggatt
     3781 cggcgcagct gacggccgcc tgattggcct gcagaagccg gcccaaaacg cgggctaggg
     3841 ccagggtgtt ggcgggaaat agagtggtga atgggtttag cggcgctggg cgctcatcac
     3901 ttggcgcctg ttgatcgcac tttacacagt tgagtagcag ccctaggtgt tcgtcgcgcc
     3961 cttcgatatg ggtaacccag gggcgcagaa cccagggtgg attatggcgt acatgttggt
     4021 tgtggccgca ggcgagttgg gccacccagt ggttctcgtc gtcttgatgg tagccgataa
     4081 tcgcctgttg cacgttaggc tgcggcgggt ttgggcagcg ccacggcgcc ttcttgcacc
     4141 aggggtaggc gaataaagaa ggtggtgcct tcgccgggca cactctccac gctaatttcg
     4201 ccgccgaggt tggtgatgca gtcccgcgct tggtacatgc caattcccat gcctttaccg
     4261 gttttggtgg tgtcgaaggg gcggaacagg cggttttgaa tgaactcttc atccatgccc
     4321 tggccgttgt cctcaatgag caccacggca aaaccgtctt ggcgagtgat gaataggtct
     4381 acatagccgc tggatagggt ggcgtcctgg gcgttttgaa tcatatgggc gaacaccatg
     4441 gtgaggcttt cgcggtcagc cttcacttgc aggtcgccgt cgtcgatgcg cagggtcggc
     4501 gctggcatgg ctttttgaca gcgtttgacg gcgtccatta acacttccga cagcaacagc
     4561 acgcgcacct cttcgccgct ggtgtgctgc agtttttgca gcaggtgttg catgcggttg
     4621 acggagttgc tgatggtttg gatggcgtct tccacgaagg cggggttgtc tttgtgtttg
     4681 gcggcgtttt tcaccaccag ggactgctgg gcgatgaggt tcttcaggtc gtgcatcatg
     4741 taggcggaga ggcggttaaa ggtttcaaac tggcgcgcct ccgccaattg atccgcctgg
     4801 gcctggcggg aaaggtagtg agccacctgg cggccgagga ttttcagcag gtccaaatcc
     4861 tcccagttaa gcgcctcttg gttttgcgcc ggtgcaatca ataccataaa gccgatcagc
     4921 tcagctttgt cgatcagcgg cagaatcagc cagagattgg tcatatcctt tgcccagttg
     4981 ggcagctgct cattgggggt ttggttttgt gaggcgctgc cggcctgggg gaaaaacacc
     5041 cattcctccc gctccagcgc gcgaataaaa gggctatcgc tgggttcttg ggcttggcgg
     5101 ggggcgatta aatctggcgc ttgatagacc ggtacaaaca cattgccccg ttttagccac
     5161 aggccgcccg cagcgcttct gaaaatattg gtcaccgagc ttagggcgcg ctggtacacc
     5221 tcttcggaat tggagggttt ggagagttgg tcgatgacgt tgagccactc ggtgcggtag
     5281 tcgtatttgt ggctgaacag gtgtttgttc accaacacgc tcagctgctc ccgcaggcgg
     5341 ctggagctga aagcgctggc cagtagaatc agcgtgccca ccagcaagag tgaatagata
     5401 atggtgcccc aatcgccgcc gtacagactc atgtagtaac ccgcaaagct aatcgcc
//
LOCUS       DAOQGS010000259         5456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7594_length_5456_cov_4.032772, whole genome shotgun sequence.
ACCESSION   DAOQGS010000259 DAOQGS010000000
VERSION     DAOQGS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7594_length_5456_cov_4.032772"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="serB"
                     /locus_tag="PKZ52_10500"
     CDS             <1..944
                     /gene="serB"
                     /locus_tag="PKZ52_10500"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNG60244.1"
                     /translation="KERHIVTLLGRQISAEDIAELTAILANHGLNIDAMTRLSGRTPL
                     DIDPLADQTQACIEFSARGTPKDSSQLRADFTALSARLNVDIAFQADNMYRRTRRLVC
                     FDMDSTLIEAEVIDELAKAAGVGDEVAAITEAAMRGELDFKQSFARRMALLKGLDEAV
                     LAQVAASLRFTEGCERLMATLKKLGYKTAILSGGFNYFGRFIQARLGMDYVFANELEI
                     VDGKVTGRVVGDVVDGQRKAQLLRELAQKENISLEQVIAVGDGANDLPMLSVAGLGIA
                     FRAKPLVKASAKQAISTLGLDAILYLMGLRDRDITQA"
     gene            1144..2511
                     /gene="prsR"
                     /locus_tag="PKZ52_10505"
     CDS             1144..2511
                     /gene="prsR"
                     /locus_tag="PKZ52_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNG60245.1"
                     /translation="MNKSSEKEKLLIIEDDIGLQSQLRWHFDQYEVIIAGNRDDAIAA
                     VRLHEPPIILQDLGLPPDDEGVEEGFRCIREISALLPTAKIIVMTGNSDYENAVRAIA
                     MGAYDFYQKPVNAETLDLILQRAFHIHRLEEQNRILKEQQQAPLDGIIACDPAMLKIC
                     RTIEKLAPSNVTCTLTGESGTGKEVLARALHRLSPRAQGRMMAINCAAVPENLMESEL
                     FGYEKGAFTGANKRTIGKIETANEGTLFLDEIGDMPLPLQAKLLRFLQERTIERVGGR
                     EDIPVDVRVVCATNKNLQQMVKDGTFREDLYYRICEMEIHIPPLRDRQGDKLLLARHF
                     LRIYAEQNNPSVVGFSQEAAESIEAYEWPGNIREMENRVKRAVVMCEDKHISSIDLGL
                     MSSDGVSINLRQVRLQAERSAILKALTMTENNISAAAKLLGVTRPTLYDLLKKYNVNL
                     PGETG"
     gene            2676..3146
                     /locus_tag="PKZ52_10510"
     CDS             2676..3146
                     /locus_tag="PKZ52_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG60246.1"
                     /translation="MKYLILIALILLTSCSTERPTKVKQDEDCWSYIRNAVKFRADKN
                     YPAALSEIEKHNSCDLKAGSDSRMSYFYHLGWTYYEMGEYDRAIAAFTEGLKTQPDYL
                     HAYVRRGLAFEKIGNFKSANNDFSRAYKLGMEGDSQKFLEYLEKNPEIKAKLIK"
     gene            3277..4095
                     /gene="lepB"
                     /locus_tag="PKZ52_10515"
     CDS             3277..4095
                     /gene="lepB"
                     /locus_tag="PKZ52_10515"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNG60247.1"
                     /translation="MANKWFSALLGFFVPPLAFVYLAKLRIAAIFFLLMVCSVAADYY
                     VASMVGFAFFALLLSVVSAVYAFVLAKKVQFEGIRKWYSWWWGVLSIPVTFFSLVFLI
                     RSFAVEPFAIPSSSMSPTVEVGDHILVYKWGYGIYGSYGFTLVESKTQNRTKPKWGDV
                     MVLNPPHDSRPFVERIVGLPGDVVEFDNKQLFINDLPVETENLGGGLVKEKMGDNVFT
                     VKYINESSQLRSGRWVVPDGHYFVMGDNRDNSLDSRAWGMVPAENIMGRVLLKW"
     gene            4235..4423
                     /locus_tag="PKZ52_10520"
     CDS             4235..4423
                     /locus_tag="PKZ52_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60248.1"
                     /translation="MRKLEIQGAANTLAPALTQRRELGFEFSRSDVSDEVCEAQNETC
                     RLIGKDALEFLGVARQSR"
     gene            4514..>5456
                     /gene="apbC"
                     /locus_tag="PKZ52_10525"
     CDS             4514..>5456
                     /gene="apbC"
                     /locus_tag="PKZ52_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNG60249.1"
                     /translation="MASLHDSALALLAQVPLEDGLGLSAADGLVAIQTQGGQQVAQIQ
                     LGYPIDGIADGLAQQLTQVLAPLGLQVELGWQAPSVAAANSQNAVPGVRNIVAVASGK
                     GGVGKSTTAVNIALALAAEGARVGFLDADIYGPSGAHMLGVTGVRPLAHAANIMQPIP
                     AYGIHMISMGNLITDDTPVVWRGPMVSGALQQLLRNTHWEDIDYLIIDMPPGTGDIQL
                     TLAQTIPVSGALIVTTPQDIALLDAKKGIEMFRKVNIPVLGIVENMSLHICSNCGHQE
                     AIFGSDGGAQLAEQYAAPLLGQLPLQLAIRAQTDAGRP"
BASE COUNT         1341 a         1321 c         1459 g         1335 t
ORIGIN      
        1 gtaaagagcg ccacatagtc accctgctcg gccgccaaat tagcgccgaa gacatagccg
       61 agctaaccgc catcctcgcc aaccacggct tgaatatcga cgccatgacc cgcctctccg
      121 gccgcacccc gctggatata gacccgctgg cggatcaaac ccaagcctgt atcgaattct
      181 ccgcccgggg cacacccaaa gacagcagcc aattgcgggc ggactttacc gcgctttcgg
      241 cgcggctcaa tgtggatatc gccttccaag cggacaacat gtaccgccgc acccgccgcc
      301 tggtgtgttt tgatatggac tccaccctga ttgaggcgga agtcatcgat gagctggcca
      361 aggccgccgg agtgggtgat gaagtggcgg ccattaccga agcggccatg cgcggcgagc
      421 tggattttaa acagagtttt gcccgccgta tggcgctttt aaagggcttg gatgaagccg
      481 tgttggccca ggtggcggcc tccctgcgct ttaccgaggg ctgtgagcgc ctgatggcca
      541 ccttaaaaaa actcggctac aaaacggcga ttttatccgg cggctttaat tatttcggcc
      601 gctttattca ggcccgtttg gggatggatt atgtgttcgc caatgaattg gaaattgttg
      661 acggcaaggt gaccggccgc gtcgtgggcg atgtggtgga tggtcagcgc aaggcgcaat
      721 tgctgcggga gctggcgcaa aaagaaaata tcagccttga gcaggtgatt gccgtgggtg
      781 atggcgccaa tgatttaccc atgttgagtg tggccggttt aggtattgcc tttcgcgcca
      841 aacccctagt aaaagcctcc gccaaacagg ccatttccac cctcggcctg gacgccattt
      901 tgtatttaat gggcctgcgg gacagagata ttacccaggc gtaattaagt gtgtttgaag
      961 gcgccgccta gggcgctcag ttaacgagtt gctcttagcg gcgccattgg ttaaggtgct
     1021 tgcgcactgt aggcacgcac tgccgggggc cgccaatgcc ctgttcagca tgcgtaccac
     1081 ccggcggcca taaataatta agcgtgcctc accccgttgt cgatcccaca cagttcaggc
     1141 cgaatgaata aatccagcga aaaagaaaag cttttaatca tcgaagatga cataggtcta
     1201 caaagtcagc tgcgttggca ttttgatcaa tacgaagtaa tcattgccgg caaccgcgac
     1261 gacgccattg ccgccgtacg cttacacgag ccgccgatta ttttgcagga tttaggcctg
     1321 ccgccggatg acgagggggt agaggagggc tttcgctgca ttcgggaaat atctgccctg
     1381 ctgcccaccg ctaaaattat tgtcatgacg ggcaatagcg attatgaaaa cgccgtgcgc
     1441 gccatcgcca tgggcgccta cgatttttac caaaaaccgg ttaacgccga aaccctcgat
     1501 ttaattctgc agcgggcatt tcatattcac cgcttagaag aacaaaaccg cattttaaaa
     1561 gagcagcaac aggcaccttt ggatggcatt atcgcctgcg atccggccat gttaaaaatc
     1621 tgccgtacca ttgaaaaact cgctccttcc aacgtgacct gtaccctcac tggcgaaagc
     1681 ggcactggta aagaagtgct cgcccgcgca ctgcaccgct taagcccccg cgcacagggc
     1741 cgcatgatgg ccattaactg cgccgcagtg ccggagaatt taatggagag cgaattattc
     1801 ggctacgaaa aaggcgcttt tactggcgcg aataaacgca ccatcggcaa aattgagacc
     1861 gccaacgaag gcactttatt tttagatgaa attggcgata tgccgctgcc actgcaagca
     1921 aaattactgc gctttttgca agagcgcacc attgagcggg tgggcggccg ggaagatatt
     1981 ccggtggatg tgcgggtggt gtgcgccacc aataaaaacc tgcaacaaat ggtaaaagac
     2041 ggcacctttc gggaagattt gtattaccgc atctgtgaaa tggaaattca cattccaccc
     2101 ctgcgggacc gccagggcga taaattatta ttggctcgcc attttttgcg tatttacgcc
     2161 gagcaaaata atccctctgt agtgggtttt agccaagagg cggcggaatc catcgaagct
     2221 tatgaatggc ccggcaatat tcgcgaaatg gaaaaccgcg ttaaacgggc ggtggtgatg
     2281 tgtgaggaca agcatatcag ctccatcgat ttaggcctga tgagcagcga cggcgtgagc
     2341 attaatttgc gccaggtgcg cctgcaggct gagcgcagcg ccattttaaa agccctcacc
     2401 atgacggaaa acaatatctc cgccgcagcc aaactcttag gggtgacccg gccaacgctg
     2461 tatgatttgt tgaaaaaata taatgtgaat ttgccggggg agacggggta atgctccacg
     2521 cccacggcaa ttcttgtaca caaattgcga aatgaccgta gggctgactc atttgcaaaa
     2581 cattggagtc ggcttttttg ttttaggatt taaaaacctc tatataaaac tatggggcct
     2641 aactttaggt tggtcttaaa aataagtggt gagtaatgaa gtatttgatt ctaattgcat
     2701 taatcctatt gacgtcgtgt agcaccgaaa ggccaacaaa ggttaagcag gatgaggatt
     2761 gctggagtta tattcgcaac gccgtaaagt ttcgtgcaga taaaaattat cccgctgctt
     2821 tgtcagaaat agaaaaacat aatagctgcg accttaaggc tggatctgac tcaagaatgt
     2881 cgtattttta ccaccttggc tggacctatt acgagatggg cgaatacgat agggcgattg
     2941 cggccttcac tgagggttta aaaactcagc ccgactattt gcatgcctac gtgcgtcgcg
     3001 gactagcttt tgaaaaaatc ggcaatttta aaagcgctaa caatgatttt tctagggctt
     3061 ataagcttgg aatggagggt gattcgcaaa aatttctcga atacctagag aaaaatccag
     3121 aaattaaagc caagttaata aaataataat gccatgttcc cacgctcctg cgagggagcg
     3181 ccctctaccc caatctgtta agccgcaaaa cctagaaggt taggatgcgg gtatcttcgg
     3241 gctgcgatat aaattttaag agagtaatca gtatttatgg ccaataaatg gttctctgcg
     3301 ttattaggtt tttttgttcc tcctttggct tttgtatatt tggctaaatt acggatcgcc
     3361 gcaatatttt ttctgctaat ggtatgttcc gtagctgctg actattacgt ggcatcaatg
     3421 gtaggttttg ccttctttgc gcttctgctg tccgtcgtgt ctgcggttta tgcctttgtt
     3481 ctcgctaaga aagttcaatt tgagggtatt agaaagtggt atagctggtg gtggggtgtt
     3541 ttatcaatac cggtaacttt cttttcgctg gttttcctaa taaggtcttt tgcggtggag
     3601 cctttcgcaa ttccttcatc atccatgtct ccaaccgttg aagttggaga ccatattttg
     3661 gtttacaaat gggggtatgg aatatatggc tcgtatggtt ttacattggt agagtctaaa
     3721 actcagaaca ggacaaagcc taaatggggg gatgtcatgg tgcttaatcc accccatgat
     3781 tctcgcccgt ttgtagagcg tatagtcggg ctgcccggtg atgtggttga atttgataac
     3841 aaacaattat ttatcaatga cttgcctgtg gaaactgaaa acttaggcgg cggattggtt
     3901 aaagaaaaaa tgggtgataa tgttttcacc gtaaagtata taaacgaaag cagtcagctg
     3961 cgctcaggac gatgggtggt tcctgatgga cattattttg taatgggtga taatcgcgat
     4021 aattccttgg atagcagggc ttggggtatg gtcccagctg aaaatattat gggaagagtt
     4081 ttgttaaagt ggtagccgaa tgaaagcagc taagattata gcggagccgc tctaatgttt
     4141 gatgcgtcga aaaaactaat taaaatggtt ttttggaggt gacaaaagaa gacagctagt
     4201 tcggttgcac ctattagttt tgagtgaatt gcgcatgaga aaattggaaa ttcaaggtgc
     4261 cgcgaatacc ctggctcccg ctttaaccca gcggcgcgaa ctaggatttg agttttctcg
     4321 ctcagatgtt tctgatgagg tttgcgaggc ccaaaacgaa acttgccgat taatcggcaa
     4381 ggatgctctt gagtttttgg gggtggcgag gcaaagccgc taactaaata agcgccccat
     4441 caatcgaaag gaattctgca aaatcatcgt caagcatggg tacaatgcct ctctttcggt
     4501 agtggaaatc accatggctt ctttacacga tagcgccctc gccctcttgg cccaagtgcc
     4561 gcttgaggac ggcctgggcc tcagcgcggc ggatgggctg gttgccatcc aaactcaagg
     4621 cggccagcag gtcgcgcaga ttcaactggg ctaccccatc gacggcatag ccgacggcct
     4681 cgcccagcag cttacccagg ttttagcgcc gctgggcctg caagtggagt tgggctggca
     4741 ggcgcccagt gtggcggcgg ccaatagcca aaacgccgtg cccggcgtgc gcaatattgt
     4801 ggcggtggcc tccggcaagg gcggcgtggg taaatccacc acggcggtga atatcgccct
     4861 ggccctcgcg gcagaaggcg cccgggtggg gtttttggat gcggatatct acggccccag
     4921 cggcgcccat atgctggggg tgacaggggt gcggcctttg gcccacgccg ccaacatcat
     4981 gcagcccata cccgcctacg gcattcacat gatcagcatg ggcaatttaa ttaccgacga
     5041 tacccccgtg gtgtggcgcg gccctatggt gagtggcgcc ctgcagcaat tgctgcgcaa
     5101 cacccactgg gaggacatag attatttaat tattgatatg ccgccgggca ccggggatat
     5161 tcaattgacc ctggcgcaaa ctattcctgt ctccggcgca ttaattgtta ctacgcccca
     5221 ggatattgcc ctgctggatg caaaaaaagg catcgaaatg ttccgcaaag tgaatattcc
     5281 agtgctcggc attgtggaga acatgtccct gcatatttgc agtaattgcg gccaccaaga
     5341 agccattttt ggcagcgatg gcggcgcgca gttggccgag caatacgccg cgcccctgct
     5401 tgggcaattg cctttgcaac tggctattcg cgcccaaacc gacgctggcc gcccgc
//
LOCUS       DAOQGS010000260         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7601_length_5452_cov_4.496572, whole genome shotgun sequence.
ACCESSION   DAOQGS010000260 DAOQGS010000000
VERSION     DAOQGS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7601_length_5452_cov_4.496572"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKZ52_10530"
     CDS             complement(<1..767)
                     /locus_tag="PKZ52_10530"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNG60250.1"
                     /translation="MLMPHTHLVRRFSISVSVALAVLVAGAPVRAAEAPSNIELIKML
                     VEKGIISVDEAKKIIADENLKKEPDANTLRVTHVPEAVREDIAAKVREGVKADVTKEV
                     LSKAKTEGWGIAGAQPEWLRKIKLSGDFRLRVQQDTFGSDNAALSVIDYQVVNEKGSR
                     TKAADKAFINATEDRMRLRDRLRLQVDAKINEAVDFHTRIATGNALDPVSTNQTLGNY
                     GEKQSIALDQAYAVGTFFDEQLQIKLGRMPNPFMASD"
     gene            complement(827..1489)
                     /locus_tag="PKZ52_10535"
     CDS             complement(827..1489)
                     /locus_tag="PKZ52_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60251.1"
                     /translation="MKQVDKPSKKLRLLVFVGGMLMTAATATGIYYLRVYLAENSAPP
                     KKVVQQQITLVAPPPPPPPPPEPPKPEVPEEKIPDETPPEPEPTPEPAPNEAPAGEDL
                     GLDADGSGGGDGFGLVGRKGGRDFLAGGGGAGSYTNAFKEKINALLSDDEELKYQKYK
                     AILRFWVSPEGVVERFEVSQFNGDPALKKRVEAAMASIKQFGKPPDDLAQPINWQVIS
                     SL"
     gene            complement(1489..1893)
                     /locus_tag="PKZ52_10540"
     CDS             complement(1489..1893)
                     /locus_tag="PKZ52_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG60252.1"
                     /translation="MKAQEDLAAYDEINVTPMLDLAYVLLVIFIIMTTATVQGIKVDL
                     PKASSTPSLAKPKTKAISIAADGTVYLDTFPVSMAELENLLRQYKAADPTLPVIIKAD
                     SQIPYQNVVDVLDLMGRLEITQLGLVTKKLVK"
     gene            complement(1899..3692)
                     /locus_tag="PKZ52_10545"
     CDS             complement(1899..3692)
                     /locus_tag="PKZ52_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HNG60253.1"
                     /translation="MKHLLTIILLLTPLWAQAWWNDEWTYRLPLVVDTTKTGGSLPQV
                     QHQVPVLVRLHAGNFDDFFSVQENLSDLRFVAEDDKTPLNFQVEHFDLISQVLLVWVV
                     VPEIAPDKTAKLYMYYGNAKAAAIDAAAPIYDPATALVVHFNDAQGVPADASGNKIPL
                     AFQGGEHSTAGLIGGAAKLTPAAQLTAQNTGALRWAAATGGAVNFWLKVPAATQGRVL
                     TFNTPAETLRLDLIDTSLSLQTNSAKITAANAIAPDQWQMVSILFANGQLQLLVNGRS
                     QAQGPLTAGELAGNLAFGQDASAAGIKGEIDEVTFYTKALAPSFIEFLAKNQGMNDTF
                     LKIEKSEQLGAGGDAGVYKTIINSMDHISWVVVCILAVMMVIGWIIMIAKYFYLRAVN
                     KDNQAFLREYEKLGTRDPALLDYDLEGNKDAYADMGPIIQALFGKHDHYQSSPIFHLF
                     HRGMKEVKARTGATLSAEAITVLGPQAINAIRAALDAQMTREIQTMQAKMTFLTMSVS
                     GGPFIGLFGTVAGVMITFGAIAAAGDVNINAIAPGVSAALVATVGGLLVAIPAMFGYN
                     FLAGIIKDTIADMRVFADEYVAKLAEYYGEH"
     gene            3893..4636
                     /locus_tag="PKZ52_10550"
     CDS             3893..4636
                     /locus_tag="PKZ52_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNG60254.1"
                     /translation="MLIRSHSADLATATGPMRTYVYEPVEAGKYPTLIFYSEIFQQTG
                     PIRRAAQMLAGHGFIVLVPEVFHELNPIGCVLGYDDAGRDKGNADKAAKYLEAHDTDT
                     QALIAYSDSLAHSNGAIGAMGVCLGGHLAFRAALNPRILAASCFYATDIHTGGVIPAQ
                     PGNESRTLAGTIKGALQMVWGKQDPHIPAASRAEVYQLLSNHNLNFTWHEFNGQHAFM
                     RDEGERYDAELHLLGFQMAIGFFQRYLKR"
     gene            complement(4758..5078)
                     /locus_tag="PKZ52_10555"
     CDS             complement(4758..5078)
                     /locus_tag="PKZ52_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60255.1"
                     /translation="MIFLVVVIAVWVRQACASSVVTAKIVNFGVYGYGNIQLELDTLI
                     DQSNCAMKYIELPSGSPIANHVMSIAALAFATGNRIQVVTDSCYNGVPSLSAGRSSYI
                     LLIK"
BASE COUNT         1387 a         1427 c         1190 g         1448 t
ORIGIN      
        1 aaatcactgg ccataaatgg attgggcatg cggcctagtt ttatttgcag ttgctcatca
       61 aaaaaagtcc ccactgcata ggcttgatct aaggctatgg attgtttttc gccgtaatta
      121 ccgagcgttt gattagtgct gacagggtca agcgcgttac ctgttgctat acgcgtgtga
      181 aaatcaactg cttcatttat ttttgcatcc acctgtaacc gtaggcgatc acgcagccgc
      241 atgcgatctt ctgtggcatt aataaatgct ttatctgccg ctttagtgcg gctgcctttt
      301 tcattaacca cctgataatc aatgacagaa agcgcggcat tatcgctacc aaacgtatcc
      361 tgttgtacgc gcaatcggaa atcaccactg agttttatct tgcgcagcca ctcaggttgc
      421 gcaccagcaa tgccccagcc ttccgtttta gctttgctta acacctcttt ggtcacatca
      481 gcttttacgc cttcgcgcac ttttgctgct atatcctcgc gcaccgcttc cggcacgtgg
      541 gttacccgca aggtatttgc atccggctcc ttttttagat tttcatcggc aatgattttt
      601 ttagcttcat caacgctgat aatacctttt tccactagca tttttattaa ttcaatattg
      661 ctcggtgcct cagccgcgcg cacgggtgcg cccgcaacta aaacggccaa agcgacggaa
      721 acgctaatac taaaacgacg aaccaaatgg gtgtggggca ttaacatgaa tacaatcacc
      781 taacagaatt taaatgcgca gcggtttaaa ataccacgct ggttttttat aaacttgaga
      841 taacctgcca attaatcggc tgcgccaaat catcaggcgg tttaccaaat tgtttaatgc
      901 tggccatggc tgcttccacc cgttttttta aggctggatc gccgttaaac tggctgactt
      961 caaagcgctc tactacgcct tcggggctca cccaaaagcg cagtatggct ttatattttt
     1021 ggtactttaa ttcttcatcg tcacttaata aggcgttgat tttttcttta aaggcgttgg
     1081 tataactgcc cgcaccgcca ccaccggcta gaaaatcccg gccgcctttg cgccccacca
     1141 aaccaaagcc atcaccgcca ccactgccgt ctgcatctaa acctaaatct tcccctgccg
     1201 gtgcttcgtt aggcgcaggc tctggtgtgg gttctggttc aggcggcgtt tcgtcaggta
     1261 ttttttcttc cggtacttcg ggttttggag gttcgggtgg gggcggaggc ggcggaggcg
     1321 gcgccaccag ggttatttgc tgttgaacta cttttttcgg cggagcgctg ttttccgcca
     1381 ggtatacacg taaataataa atgcctgttg ctgtggcggc ggtcatgagc atgccgccaa
     1441 caaataccaa caagcgtaat tttttcgaag gcttgtctac ctgcttcatt atttaaccag
     1501 ctttttggta accaaaccca gctgggtaat ttccaggcgc cccattaaat ccaaaacatc
     1561 cacaacattt tggtaaggta tctgagaatc ggctttaata atcacaggca gcgtaggatc
     1621 tgccgcctta tattgtcgca agagattttc taattccgcc atggataccg gaaaggtatc
     1681 caaataaacc gtgccatctg cagcaatgct aatggctttg gttttgggct ttgcaaggct
     1741 aggcgtgctg ctggctttag gtaaatccac cttaatacct tgcactgtgg cggtggtcat
     1801 aataataaat atcaccaaca gcacataggc taaatccaac atgggggtta cgttaatttc
     1861 atcgtaggcg gctagatctt cttgcgcttt catggcaatt aatgctcccc gtaatattct
     1921 gctaatttag ccacatactc atcggcaaat acccgcatat cggcgatggt gtctttaatg
     1981 ataccggcta aaaagttgta tccgaacatg gccggaatag ctaccaataa accgcccacc
     2041 gtcgccacca gggctgccga aacacccggc gcaatagcat taatattcac atccccggca
     2101 gcggcaatgg caccgaaggt tatcattaca cctgccacag ttccgaatag tccaataaat
     2161 ggcccaccgc ttacggacat ggttaaaaat gtcattttgg cctgcatggt ttgaatttca
     2221 cgggtcattt gtgcatctag cgcagcgcgg atagcattta tagcttgcgg gcccaacacc
     2281 gtaatcgcct cagcactgag agttgcacca gtgcgggctt ttacttcttt cataccgcga
     2341 tgaaataaat ggaaaatcgg cgagctttgg tagtggtcgt gtttgccaaa taacgcctga
     2401 ataattggcc ccatatcggc gtaggcgtct ttattacctt ccaaatcata atccagtagg
     2461 gctgggtcgc gagtgccgag tttttcatac tcgcgcaaaa atgcctggtt atctttattg
     2521 accgcacgca aataaaaata tttggcgatc ataataatcc agccgattac catcatcacc
     2581 gcgagaatac acaccaccac ccagctgata tggtccatgc tgttgataat ggttttatac
     2641 acacccgcat cgccaccagc acctaattgt tcggattttt caattttaag aaaggtgtca
     2701 ttcatgccct ggtttttggc caagaattca ataaagctgg gggctagtgc cttggtgtaa
     2761 aaagtcactt cgtcgatttc gcctttaatc cctgctgcgc ttgcgtcctg accaaaggct
     2821 aaattgcctg ctaattctcc agcagttaat gggccttgcg cctgggagcg accattgacc
     2881 aataattgca gctggccatt ggcaaataaa atgctcacca tttgccactg atcaggggct
     2941 atggcattag cggcggtgat ttttgcgcta ttggtttgca ggcttaagct agtatcaatt
     3001 aaatccaggc gcagtgtttc ggccggagta ttaaatgtca gcactcggcc ttgagtggcg
     3061 gcaggcactt ttaaccaaaa attcaccgcg ccgcccgtag cagccgccca gcgcagagcg
     3121 ccagtgtttt gtgcggttaa ttgcgcagcc ggagttaatt ttgccgcccc gccaattaac
     3181 cctgctgtgg aatgctcacc accttgaaag gcaaggggaa ttttattgcc actggcatct
     3241 gcaggcacac cctgcgcatc gttaaaatgc accactaagg cagttgcggg atcataaata
     3301 ggcgccgccg catcaatcgc agctgctttg gcattgccat aatacatata gagtttggcg
     3361 gttttatccg gtgctatttc aggcaccacc acccacacca ataatacttg gctaattaaa
     3421 tcaaagtgtt caacttgaaa atttagtggg gttttatcgt cttcagcaac aaagcgcaga
     3481 tcgcttaaat tttcctgcac ggagaaaaaa tcatcaaaat tccccgcatg caggcgcact
     3541 aataccggca cttggtgttg cacttgcggc agggagccac cggttttggt agtatcaacc
     3601 accaacggca aacgataagt ccactcatcg ttccaccagg cctgtgccca aaggggcgtt
     3661 agcagcagaa taattgttaa taaatgcttc atggccagct ccaaaaactc tttgcagcag
     3721 gctagcaggg caatattgcg gttttatgcc ggttttgtta ccaatgttac aaccgcatac
     3781 accagtgcct tgcctccgcc gaacctatct gcgcggtgtt tctggctgcc cgcatccgct
     3841 aatatgacaa accgattaca cacgccacaa tttcccagta cgagacctca ttatgcttat
     3901 tcgctcccac agcgccgacc tggccaccgc caccggcccc atgcgcacct atgtttatga
     3961 acctgtggag gcaggcaaat accccacact gattttttac tcggaaatat tccagcaaac
     4021 cggccctatc cgccgggccg cacaaatgtt ggccggccat ggctttattg tgctggtgcc
     4081 ggaagtcttc cacgaattaa atcccatcgg ctgtgtttta ggttatgacg atgccgggcg
     4141 ggacaagggc aatgcggata aagccgctaa atatttagaa gcccacgata cagacaccca
     4201 agcgcttatc gcctatagcg atagcctcgc ccacagcaac ggcgccatag gtgccatggg
     4261 tgtgtgttta ggcggccacc tcgcctttcg cgccgccctc aacccgcgca tcctcgctgc
     4321 tagctgtttt tacgccacgg atatacacac cggcggcgta atacctgcgc aaccgggcaa
     4381 cgaatcccga actcttgctg gcactattaa aggcgccttg caaatggtct ggggcaaaca
     4441 ggacccacac attcctgcgg cctcaagggc ggaggtttat caactgctca gcaaccataa
     4501 tttgaatttc acctggcacg aatttaacgg ccagcacgcg tttatgcgcg atgaagggga
     4561 gcgttatgat gcggaattgc atctgttggg gtttcagatg gcgattgggt tttttcagcg
     4621 gtatttgaaa cgctaataag catgcagatt tactcaataa tgggaccacc ccatcaattc
     4681 aagcgctaaa ataataaacg ccggcacttg cacagagata aatctgcgca tgtgcaatta
     4741 tcgactcgat aaaaacttta cttaatcaat aatatataag atgaacggcc agcgctaagc
     4801 gagggaacgc cgttataaca actatcagtt accacctgaa ttctattgcc ggttgcaaac
     4861 gccaatgccg ctattgacat tacgtgattt gctatcgggc taccagatgg caattcaatg
     4921 tatttcattg cgcaatttga ttgatcgata agagtatcca gttcaagctg aatattaccg
     4981 tatccataga caccaaaatt cactattttt gccgtcacca cacttgacgc acaagcctgt
     5041 cgcacccaaa ctgcaattac aacgactaaa aaaatcatta gctttccact caatttttca
     5101 ctcctagttc gcccagcagc attagcacaa tcatatgaaa ccacaattcg gctctccgct
     5161 tttaattaaa tctaatcaca acaagtatat ggaatttttt attggatagc attaatgcac
     5221 ccggccaatc tgttaccata agtaaattga ctgcacatta gtcgcccagg ctggatttcc
     5281 gcaacctgcg ttatctattt ctatttccac atcccgacct gacaccagag ctgttaaggc
     5341 tatcgaaaag ctttccttac caccgcccca tcccaatggg attttataca tattagtagg
     5401 cagattcaac tccgaagtgc atcacagccc ccggctagaa aaagaagcca gc
//
LOCUS       DAOQGS010000261         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7602_length_5452_cov_4.356494, whole genome shotgun sequence.
ACCESSION   DAOQGS010000261 DAOQGS010000000
VERSION     DAOQGS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_5452_cov_4.356494"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..926
                     /gene="ribA"
                     /locus_tag="PKZ52_10560"
     CDS             297..926
                     /gene="ribA"
                     /locus_tag="PKZ52_10560"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNG60256.1"
                     /translation="MNSMLPTQITRLACARIPTRHGDFLLYYYGNSIDTKEHLAFCFG
                     DVSEAEAVLVRVHSECFTGDVLGSLRCDCGEQLDRALAAIAAAGRGVVVYLRQEGRGI
                     GLLEKLRAYNLQDAGFDTLEANIMLGHGADEREYSLAAHILADLSVRSVRLLTNNPAK
                     IRALIDAGVTIIDRVALEISANPENIAYLATKAKRMEHLLNDLSVELTP"
     gene            923..1654
                     /locus_tag="PKZ52_10565"
     CDS             923..1654
                     /locus_tag="PKZ52_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNG60257.1"
                     /translation="MNLDVNLTSWLSQQAHKKIAARPFVTLCYAQSLDGSIALRVGTR
                     CAISSPESNVLTHQLRAWHDGILVGIGTVLSDDPQLNVRHSLGKNPQPIVLDSHFRIP
                     PRARLRHGNKKCWVASCQSPAADADLNGVELIYTPHNSEGQVCLASLLASLYNKGIRR
                     LMVEGGAEVIRAFLRAQLVDAVVITVAPTFLGGYKSLNNLDLPAEQLPTIAPLHSQPL
                     GSDIIIWGPAHFAEEVKHTTRRVAL"
     gene            1671..2684
                     /locus_tag="PKZ52_10570"
     CDS             1671..2684
                     /locus_tag="PKZ52_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNG60258.1"
                     /translation="MHAQQLWFHCKKELEVRTINLAPLQADEVLVASECSAISVGTEL
                     LAYREQLPTDIPLDEQINGLNHTVEFPLRYGYASVGRISAVGSEADGKLIGQRVFAFE
                     AHCSHFIARRDDVIAVPAGITAEQAVFLANMETAVSLVQAGAPAIGEKIAVIGLGVVG
                     LLLAQLLKQFPSHRLIGLDKLSARRAAAKGVGIHDLVDPSDKGQWQSLLTQLMPEGAD
                     LIYEISGNPAALDYACALAGFSSRIIIGSWYGNKPCAVNLGGAVHRNHIQLISSQVSR
                     IAPALSGRWTKQRRFKLAWDMIRLIQPQTLIDRRAPLKSAPDIYRQLDEQPDAIVQPI
                     FVY"
     gene            2699..3076
                     /locus_tag="PKZ52_10575"
     CDS             2699..3076
                     /locus_tag="PKZ52_10575"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNG60259.1"
                     /translation="MYALSVQQTFFAQHFLIGGDWGSENQPHTHEYRWEVILTGPNLD
                     QHGYLVDIVDVEAHMQKIVARFRDTCLNQHPEFADLNPSLERFAKILWQQLKQQLTLP
                     HETCLRVKLWENATCWAGYSQAY"
     gene            complement(3423..4511)
                     /locus_tag="PKZ52_10580"
     CDS             complement(3423..4511)
                     /locus_tag="PKZ52_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNG60260.1"
                     /translation="MLRGLQLNIPGACVQLEFIRWALCVQLALLGFAGFLLQVLPGPL
                     ALRWYCQALVIFVVIAWQVWLLMRNQQLIADLAQLGLPNQVSLVRAVLILCCGGFFGL
                     NDLPAPLNWLPAILYALAAILDAVDGWLARRLNQVTQLGARLDTCFDALGLCIAPLVA
                     CALNKLHSSYLLVSVAYYIFQWGLNWRRARGLLCHPLQASENRRLWAGMQMVLVACVL
                     MPVVPAEFSRHLGAVFMLPLLAGFIRDWCAVAGIHFSRQIINIDGLGKKFYPLGLVCL
                     RGVLALSVFYGFNLHGVGQLLIILMTLCIALGIAGRLAAAVILVTLAQFNVAQPLLTS
                     VQLIVLYSAAGVFLLGTGKWSVCKQTWL"
     gene            5115..>5452
                     /locus_tag="PKZ52_10585"
     CDS             5115..>5452
                     /locus_tag="PKZ52_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60261.1"
                     /translation="MSELPANPFYKPYLLQLLAIVFFASVAAFLHFVPLIQESAPNAV
                     EKAGENKPARPAGGNARTGGPVTVQTGLAQLRDLAVVESGLGTVVPAAEVLVQARVSG
                     RLEKILFTEG"
BASE COUNT         1443 a         1399 c         1309 g         1301 t
ORIGIN      
        1 ttttttggca aattcaacga cgccagagcg agtaaggtaa tttttcagag gttccttaaa
       61 aacctcgctc attgatgggg catgtggcaa agcagcgctt taaaaacatc aacactgatt
      121 aattgtaaac acttgatgtt taaatctaac cacagcgatt atcaatataa acaggtatcg
      181 aataaattaa agtgcacact ttaaaaagac tatcagccgc cttgcctaat ggctagcctc
      241 gcgttatggt tcccggcact tatcagcatt tcttagttgg atttttatca aaccccatga
      301 acagcatgct tcccactcag attactcgcc tggcatgcgc cagaattccc acccggcacg
      361 gcgacttttt actttattac tacggtaatt caatcgatac caaagagcac ttggcctttt
      421 gttttggcga tgtatctgag gccgaagccg tactcgtgcg cgtgcactcg gaatgtttta
      481 ccggcgatgt gctgggctct ttgcgctgtg attgtggcga acaattggat cgcgcgctgg
      541 cggccatagc ggcagctggg cgcggggttg tggtgtattt gcgccaagaa ggacgcggca
      601 taggcctgct ggaaaaattg cgcgcctaca atttacagga tgcaggtttt gacaccctgg
      661 aggcgaatat tatgctgggc catggcgctg atgagcgcga atattccctc gccgcacata
      721 tattggcaga tttatccgtg cgctccgtgc gtttactcac caataacccc gctaaaattc
      781 gcgctttaat tgacgcgggc gtcaccatca ttgaccgggt ggccttggaa atttccgcca
      841 accctgaaaa tatcgcttat ttagcgacta aagccaaacg catggaacat cttttaaatg
      901 atttaagcgt cgaattaaca ccatgaatct cgacgtcaat ttaaccagct ggcttagcca
      961 acaggcccac aaaaaaatag cggcgcggcc ttttgtcacc ctgtgttatg cacagagttt
     1021 agatggcagc atcgcactgc gggttggcac ccgttgcgcc atcagcagcc cggaatctaa
     1081 tgtgctcacc catcaattgc gcgcgtggca cgacggtatt ttagtgggca tcggcacggt
     1141 gctcagcgac gacccgcagc tcaacgtgcg ccacagttta ggaaaaaacc cgcagccgat
     1201 tgtgctggac agccatttta gaattccgcc gcgggcgcgc ctgcgccacg gcaacaaaaa
     1261 atgctgggtg gccagctgcc aatcgccggc tgccgatgcc gatttaaacg gtgtggaatt
     1321 aatttatacc ccccacaata gcgaaggcca agtgtgttta gccagccttt tggcaagctt
     1381 gtataacaag ggtattcgca ggcttatggt ggagggcggc gcagaagtca ttcgcgcttt
     1441 tttaagggcc caattagtgg atgcggtggt gattacagtg gcgccaactt tcttgggcgg
     1501 ttataaatcc cttaataatc ttgatttacc ggccgagcaa ttgcccacca tagcgccctt
     1561 acacagccag cctttaggca gcgacattat tatttggggg cccgcgcatt ttgccgagga
     1621 agttaaacac acaacacgtc gcgtcgctct atagcgccta gagatttttt atgcatgcgc
     1681 aacaattgtg gtttcactgc aaaaaagaac tggaagtgcg cacaattaat cttgcgccgt
     1741 tacaggcgga cgaagtattg gtcgccagcg agtgctccgc catcagtgta ggcacagaat
     1801 tactcgccta ccgggagcaa ttacctactg atatccccct ggatgaacag attaatggct
     1861 taaatcacac ggtggaattt cccctgcgtt atggttatgc cagcgtaggc cgtattagtg
     1921 ccgttggctc tgaggcggat ggtaaattaa tcggccagcg ggtgtttgcc tttgaggccc
     1981 attgcagcca ttttattgcc cggcgggatg atgtgattgc agtgcccgca ggtattaccg
     2041 ctgagcaggc ggtatttctg gccaatatgg aaactgctgt gagcttggtg caggccggcg
     2101 cgcccgccat aggtgaaaaa attgctgtta taggtttggg cgtagtgggt ttattgctgg
     2161 ctcaattatt aaaacaattc cccagccacc gcctaatagg cttagataaa ttatcggcgc
     2221 ggcgtgcagc ggccaagggt gtgggaattc acgatctggt ggaccccagt gataagggcc
     2281 aatggcagag tttattaacc caattaatgc ccgagggcgc ggatttaatt tatgaaatta
     2341 gcggcaaccc tgcggcgctg gattacgcct gtgcgctggc cggattttcc agccgcataa
     2401 ttataggcag ttggtacggc aacaaaccct gcgccgttaa tttaggcggc gcagtgcacc
     2461 gcaaccatat tcaactaatc agcagccagg tgagccgtat tgcacctgca ttaagcgggc
     2521 gctggacaaa acagcgccgc tttaaattgg cctgggatat gattcgcctc atccagccgc
     2581 aaacgctaat tgataggcgc gcgccgctca aatccgcgcc cgatatttat cggcaactgg
     2641 atgagcagcc cgacgctata gtgcaaccta tttttgttta ttaagtggat cctcaatcat
     2701 gtatgccctg tctgtacaac aaactttttt cgcccagcat tttttaattg gcggcgactg
     2761 gggcagcgag aatcaacccc acacccacga gtaccgatgg gaagtcatat taaccggccc
     2821 caacctagac cagcacgggt atttagtgga tatagtggat gttgaagcgc atatgcaaaa
     2881 aatagtggcg cgctttcgcg acacttgcct taaccaacac ccagaattcg ccgatttaaa
     2941 cccgagcttg gagcgttttg ccaaaattct ttggcaacaa ttaaagcagc aattaaccct
     3001 tccacatgaa acctgcctaa gagtaaaact gtgggaaaat gccacctgct gggcggggta
     3061 tagtcaggct tattaacccg gcggcaaaat agatcgtcct gatctatttt gcctgcgctg
     3121 cgctaaaacc ggcgaaaatg cgcggatttt attgttttag cgcagctccc ggcggtttac
     3181 cccgccggga cggcatcctt gccgccatta ttaacccggc actaaatgcc gccgggttaa
     3241 taattacaat accgggcaat attcaataat aattcaagta gcccggatgg agcgcagcgc
     3301 aatccgggta ttgctgaaaa acgatgtgta tttattgttg tcgcgcagcg cgtatacccc
     3361 accccggatt gcgccgagct ccatccaggc taccaacaaa cccaacacct taaataacaa
     3421 catcaaagcc aggtctgttt acacacactc cacttgcctg tgcccaataa aaaaacacct
     3481 gccgcgctgt ataacactat taattgcact gatgttaata acggctgagc aacattaaac
     3541 tgcgccaaag taactaaaat aacggcggcg gctaggcggc ctgctatgcc cagcgcaata
     3601 cacaacgtca ttagaataat taacagctgc ccaacaccat gcaaattaaa tccgtaaaaa
     3661 acactcaatg ccagcacacc acgcaggcaa accaggccta atgggtagaa ttttttgcca
     3721 agcccatcaa tgtttattat ctggcggcta aaatgaatcc ccgccacagc gcaccaatcg
     3781 cgtataaaac ctgccagtaa cggcaacata aatacagcgc ccaaatgacg ggaaaattca
     3841 gctggtacaa ccggcattaa cacacaggcc accaaaacca tttgcatacc cgcccaaagg
     3901 cggcgatttt cgctggcttg caatggatga catagtaaac cccgtgcacg ccgccaattt
     3961 aatccccact gaaaaatata ataagcaaca ctgaccagca gataactgct gtgtaattta
     4021 ttcagcgcgc aggcaaccag cggtgctatg cataaaccca gggcgtcaaa acaggtatcc
     4081 aaacgcgcac ccaattgggt tacctggttc aaccggcgcg ctaaccagcc atccacagca
     4141 tctaaaatgg ccgccagtgc atataaaatt gctggcagcc aattaagtgg tgcaggcaga
     4201 tcgtttaagc cgaaaaatcc gccacagcat aagataagca ccgcccgcac taggcttacc
     4261 tgattgggca agcccagctg cgcaagatcg gcaattaatt gttgattccg cattaacagc
     4321 caaacctgcc aggctatgac gacaaagatc accaaggctt ggcaatacca acgcagcgcc
     4381 aagggcccag gcagaacttg cagcaaaaag ccagcgaagc ctaataatgc cagctgaaca
     4441 cacaatgccc agcggataaa ttccagctgt acacaagcgc caggtatgtt taattgcagc
     4501 cctcgcaaca cgtttgcacc ctaactaatg gttttaaaaa atcctgccca gcatatagca
     4561 cctggcgggc acgggcactt acttagtagg gtgggcaagc ttgcttgccc accattttgc
     4621 ttaaacgcga taccaaggtg ggcaacaagt tgcccaccct accggataaa acaggtaaat
     4681 tcgaaactca aatttgagtg attaaaaata accagtaatt tttatttacc aacagttgtg
     4741 tgcttaaaat taaccaacca ggcctgaata ttctgcatat tggcgcttat cgccagctgg
     4801 agcgcaattt gctagggccg cccaatgtgc ttaaaacatc gggtgaatta accgcgtcga
     4861 tttcgcctca tatgttttga agactcgtac tggcgcgaat ttgccaagcc gagttatttt
     4921 tacaggcata gtgcaaggct taaatagttc gcgacagcaa acatgcataa atgtaggcat
     4981 atgatgaaac gcttttgcgt gtttgctgcg ttttaaattc atacattttt ttaaatcgtc
     5041 tgcccatcgc gcggttgcgg tttcaccggt gttttaagtt gtacgcgtgc cccgcaagaa
     5101 tcagagtgtg ttgaatgtct gaattacccg ccaatccttt ttataagccc tatttattgc
     5161 aactactggc cattgtgttt tttgccagcg tcgcagcttt tctacatttt gtgccgctta
     5221 ttcaagaatc tgcacccaat gcggtagaaa aagcgggcga gaataagcct gcacgcccag
     5281 ccgggggaaa tgcccgcact ggcggaccgg taacggtaca gacgggcctt gcccaactgc
     5341 gggatttagc ggtggtggaa tcgggcctgg gtactgtagt gccagcggcg gaagttttag
     5401 tgcaggcgcg ggtttccggc cgtttggaaa aaatactgtt taccgaaggc ca
//
LOCUS       DAOQGS010000262         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7644_length_5437_cov_5.058343, whole genome shotgun sequence.
ACCESSION   DAOQGS010000262 DAOQGS010000000
VERSION     DAOQGS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7644_length_5437_cov_5.058343"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..778)
                     /locus_tag="PKZ52_10590"
     CDS             complement(20..778)
                     /locus_tag="PKZ52_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNG60262.1"
                     /translation="MTTLFTELLKDSAYRLDQFKQAHIDRLEREISLKQSGKSLVPYV
                     DCVVRGKPVKLTPEEAIRQLYLLVLRDDLGYPISRIEVEYSVTFGREKKRADICIFDK
                     DKTTTPYILVELKKPKLKDGKEQLKSYCNATGAPMGVWTNGQQISYYHRKDPNYFEDI
                     PAIPSAHEKLSDILSERWTIDDLVKLDKLVNERKSLKDLILEMEDEVLANAGVDVFEE
                     LFKLIFTKLFDEMESGQDKNRYLRNYVAAAQVFS"
     gene            1023..2873
                     /gene="ilvD"
                     /locus_tag="PKZ52_10595"
     CDS             1023..2873
                     /gene="ilvD"
                     /locus_tag="PKZ52_10595"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNG60263.1"
                     /translation="MPIYRSKTSTAGRNMAGARALWRATGMQDADFQKPIIAVVNSFT
                     QFVPGHVHLKDLGQMVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDIIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVIFVSGGPMEAGKTKLSEHKL
                     DLVDAMVIAASDTATDEEVAAYERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGT
                     VLATHSDRKELFLRAGREIVALTKRYYEGDDERVLPRAICTKKAFENAMTLDIAMGGS
                     TNTILHILALAKEAEVDFDLTDVDRLSRVVPQLCKVAPNTPKYHIEDVHRAGGIMAIL
                     GELDRGGLLHSDCHTVHSSSFTEALEQWDILRNPTPEVLQFFKAGPAGIPTQTAFSQS
                     TRWPSLDADRENGCIHSVEHAFSKEGGLAVLVGNLAEKGCVVKTAGVDESIWVFEGPA
                     HVTESQDQAVAHILEGKVKEGDVVIVRYEGPKGGPGMQEMLYPTSYLKSKGLGKYCAL
                     LTDGRFSGGTSGLSIGHVSPEAAAGGTIGLVQNGDLIRIDIPNRSINVLVSDAELAAR
                     REQQNAKGWKPAEPRPRKVSPALRAYALLATSADQGAVRDLDKLAQLEQL"
     gene            2962..4023
                     /locus_tag="PKZ52_10600"
     CDS             2962..4023
                     /locus_tag="PKZ52_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60264.1"
                     /translation="MGRLRVTVNKKTKGKKALTPQEAFAKLWKQVQKEQLLVSQIPEQ
                     IQQFYQTYLTTIGPYESQWCRLLADEIQLMLSHWQKKSLREQDRYFLVEYVADNFREL
                     MSNPFRDFELTGLEELFTELMAQAEEPKRAAKPPGTEDMFATDEEADEEIEAEDPFSA
                     YDEDFAEDELLEEEYQRAERQDANELFNATTLNKMFRQLSKVLHPDLEQDETIKQHKH
                     ELMAQLLNARDKQDVGAIVALYSEHIGGDGAQFAPEDFPRLTILLKKQLRGLQLEKAD
                     IALRSGAAGLVYHRFHHKNPARQKTLLMQRQALMASRCRAAQESLNFNTSVKNLKLFL
                     SEWAEDNLGAMLFDLYDDL"
     gene            4553..>5437
                     /locus_tag="PKZ52_10605"
     CDS             4553..>5437
                     /locus_tag="PKZ52_10605"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNG60265.1"
                     /translation="MPVKIELNNTASQGAKPVKIYTRDVDNQALAQLKNIAQLPIIHS
                     HVAAMPDVHVGIGATVGAVIPTLGAIIPAAVGVDIGCGMSAVRLTLKSAQLPDNLSVL
                     RAAIERAVPVGLAAHKTVSVRTSACKTLAPGVDWLFAKHPTVMKMLKQPNTLWTKQMG
                     TLGGGNHFVEVCVDEHKDVWIMLHTGSRGIGNAIGQYFIALAKKDMGRHMHNLPDKDL
                     AYLTEGSEHFNDYVAAVNWAQNYARINRDEIMALVVRAIKPLLPPFKTDRVAINCHHN
                     YVQREFHFGADVWVTRKGA"
BASE COUNT         1334 a         1289 c         1449 g         1365 t
ORIGIN      
        1 ggaaacaata ggggttaggc taggaaaaaa cttgtgcggc tgctacataa ttccgtaaat
       61 aacgattttt atcttgcccg ctttccattt cgtcgaagag tttggtgaat ataagtttga
      121 ataattcttc aaacacatct accccggcat tcgcgagcac ttcgtcttcc atttcaagga
      181 tcaagtcttt gagtgatttg cgctcgttga cgagtttgtc gagttttaca aggtcgtcga
      241 tagtccaacg ctctgacaga atgtcggaga gtttttcgtg ggctgaaggg atggcaggga
      301 tatcttcaaa atagtttggg tctttgcggt ggtaatagga aatttgctga ccgttggtcc
      361 atacccccat gggtgctcct gtcgcattgc agtagctttt gagttgttcc ttgccgtctt
      421 tgagtttggg ttttttgagt tcaaccagaa tgtagggagt tgtggttttg tctttatcga
      481 agatgcagat atctgctcgc tttttttctc gcccaaaggt tactgagtat tcaacttcta
      541 tgcggctaat ggggtagcct aaatcgtctc gcaagactaa taggtagagc tggcgaatgg
      601 cctcctctgg cgttagtttg acaggcttgc cgcgcacaac gcagtcaacg tagggaacta
      661 ggcttttacc ggattgtttt agtgaaatct cccgttctag ccgatctata tgcgcttgct
      721 tgaactggtc tagtcgatag gcactgtctt tcagtaattc tgtaaagaga gttgtcattc
      781 ttggtttcct gtgcgggcgt tctattgttc ttctgattaa agcaagaggg cagggattcc
      841 agagattgtt tggtcgggtt cagaatcgct gcctcaatgt gtcgttgagg tttagtgggc
      901 ggccaatcta gcactctgtg ctttctctgc aaacaaagac gagtgttatt ttcacggtgg
      961 atgagcgata gattttggta tgctgccacc ctctaacaaa agcacatcag atcgaggccc
     1021 ccatgcccat ttaccgctct aaaacctcca ccgctggccg caatatggct ggtgcccgcg
     1081 ccctgtggcg agcaactggt atgcaggatg cggattttca aaaacctatt attgctgtgg
     1141 tgaattcctt cacccagttt gtgccgggcc atgtgcacct gaaagaccta ggccaaatgg
     1201 tggcgcggga gattgaggcg gccggtgggg ttgccaaaga atttaatacc atcgccgtgg
     1261 atgacggtat cgccatgggc catgacggta tgctctacag cttgcccagc cgcgacatca
     1321 tcgccgactc ggtggagtat atggttaacg cccactgcgc cgatgccatg gtgtgtatct
     1381 ctaactgcga caaaatcact ccgggcatgt taatggccgc catgcgcctg aatattccgg
     1441 tgatttttgt ctccggcggg cctatggagg cgggcaaaac caagctttcc gaacacaaac
     1501 tcgatttagt ggacgctatg gtaattgccg ccagcgacac cgccacggat gaagaagtcg
     1561 ccgcttatga gcgcagcgcg tgccctacct gtggctcttg ctcgggtatg tttaccgcca
     1621 actccatgaa ctgcctcacc gaagctttgg gactttccct gccgggcaat ggcacggtgc
     1681 tggccaccca tagcgatcgc aaagaattat tcctgcgcgc tgggcgcgaa attgttgccc
     1741 tcaccaagcg ttattacgaa ggcgatgatg agcgcgtatt gccccgcgcc atttgcacca
     1801 aaaaagcctt tgaaaacgcc atgacgttgg atatcgccat gggcggctcc accaatacca
     1861 ttttgcatat tctggccctg gctaaagaag cggaagtgga ttttgattta accgatgtag
     1921 accgcttaag ccgcgtggtg ccccagttgt gtaaggtggc cccgaatacc cccaaatacc
     1981 atatagaaga tgtgcaccgc gccggtggca ttatggcgat tttgggggag ctggaccgcg
     2041 gtggcctgtt gcacagcgac tgccacacgg tgcacagcag cagttttact gaggcccttg
     2101 agcagtggga tattttgcgc aacccaacgc ctgaggtttt gcaattcttt aaagccggcc
     2161 ccgccggtat tcccacgcaa accgccttta gccagagcac ccgctggcca agcctggatg
     2221 cggaccgaga aaacggctgt attcacagtg tggaacacgc cttctctaaa gagggaggcc
     2281 ttgcagtttt agtgggtaat ttggccgaaa agggctgtgt ggtaaaaacc gccggggtgg
     2341 acgagtccat ctgggtattt gaagggcctg ctcatgtcac cgaaagccag gatcaagccg
     2401 ttgctcatat tctggagggc aaggttaaag agggcgatgt ggtgattgtg cgctacgaag
     2461 gccccaaggg tggcccgggt atgcaggaga tgctctaccc cacgtcttat ttgaaatcca
     2521 aaggcctggg caaatactgc gccctgctta ccgacgggcg ctttagcggt ggcacctcgg
     2581 gcttgtctat cggccatgtc tcgccggagg cggcagcagg cggcactatt ggcctggtac
     2641 aaaacggcga tttgattcgc atcgatatcc ccaaccgcag cattaatgta ttggttagcg
     2701 atgctgagtt agccgcgcgc cgggagcagc aaaatgcgaa gggctggaaa cccgccgaac
     2761 cgcgcccgcg caaagtctcg ccagctttgc gcgcctatgc attgctggcc accagcgcgg
     2821 atcagggcgc ggtgcgggat ttagacaaac tcgctcagtt agagcagcta taactgctcg
     2881 cgttttggta aataaaagcc ccagtattgg ggctttttta ttggtagctg cagcatttag
     2941 tgaagattta agaggttttg catgggccga ttacgggtta cagtcaataa aaaaaccaag
     3001 ggtaaaaaag ccttaacacc ccaggaggcc tttgccaaat tatggaagca ggtgcaaaaa
     3061 gagcagttat tggtaagcca aattcccgag caaatccaac agttttacca aacctattta
     3121 actaccatcg gcccttacga atcccagtgg tgccgcctat tggctgatga aattcaactc
     3181 atgctcagcc actggcagaa aaaaagcctg cgcgagcaag accgttattt tttagtggaa
     3241 tacgtggcgg ataatttccg cgaattaatg agcaacccct tccgtgattt tgaattaacc
     3301 ggacttgagg agctgtttac cgagttaatg gcgcaagctg aagagccaaa acgcgccgct
     3361 aaaccgcctg gcactgagga tatgtttgcc acggacgaag aggcggatga agaaatagag
     3421 gcggaagatc cgttcagtgc atatgacgag gattttgccg aggatgaatt attggaggaa
     3481 gaataccagc gtgccgagcg ccaggacgcc aacgaattat ttaatgccac gactttaaat
     3541 aaaatgttcc gccaattaag caaggtatta cacccggatc tggagcagga tgagaccatc
     3601 aaacaacaca aacacgaatt aatggcgcaa ctgctcaacg cccgcgataa gcaggatgtg
     3661 ggcgccatag tggcgctcta tagtgagcat ataggcggcg atggtgccca gtttgcgccg
     3721 gaggatttcc cccgcttaac tattttgttg aaaaaacaat tgcgcggcct gcaactggaa
     3781 aaagcagaca tcgccctgcg ctctggagcg gcgggcttgg tgtaccaccg cttccaccat
     3841 aaaaacccgg cccggcagaa aaccctatta atgcagcgcc aagcgctgat ggcaagccgc
     3901 tgccgcgcgg cgcaagagtc cttaaacttt aataccagcg ttaaaaacct gaaattattt
     3961 ttaagcgaat gggcggagga taatttgggt gcgatgctgt ttgatttgta cgatgactta
     4021 taacgatcac gcccaatatt gcctcacagc ccttagtcaa aaaccctaac cattgggtat
     4081 ttttattggc ctgggtgtaa ttcccgcgca aactgccaag gcccataggt gtaatctggt
     4141 ttgaatagct gcctcgcggc gaaggaagtg cgcgttggag tttgtgcttt taagttccgc
     4201 atggatggaa gagtaaataa tgtgccgggc gataaaaaat ctaaagatgc atattgacga
     4261 acaaaaaatt cagcgtatta tcgcgcccaa tttccggcag ggcacacctg cttttttaac
     4321 ccacccaatg atagaggagt actcattatg gcctacgcga acttaactaa ccgctaacct
     4381 ttcgagtact caggcttgga aggtttaatg ccttctaagc aggtctttaa aagccctgat
     4441 taggctacta atcagggctt ttttgttttc tgggccttat tggccgcaag cctactgtga
     4501 aggttttatt attcaaacac ggcgattatt aacaatcgct gtaggaattt ttatgcctgt
     4561 taaaattgaa ttaaataaca ctgccagcca gggtgccaaa ccggtaaaaa tttatactcg
     4621 ggatgtggat aaccaagccc tggcacaatt aaaaaatatc gcccaactac ccataatcca
     4681 cagccatgtg gcggccatgc cggatgtgca tgtggggatt ggtgcaaccg tcggcgcggt
     4741 gattcccacc ctcggtgcaa ttattcctgc cgccgtgggt gtggatattg gttgcggtat
     4801 gagtgcggtg cgtttaaccc ttaaatccgc gcaactgccg gataatttaa gtgttctgcg
     4861 cgcggcgatt gagcgagcgg taccggtggg tttagcggca cataaaaccg tgagtgtgcg
     4921 caccagtgcc tgtaaaaccc tggcacctgg tgtggattgg ttattcgcca aacatcctac
     4981 cgtgatgaaa atgttaaaac aacccaatac cctatggacc aaacaaatgg gtaccttggg
     5041 tggtggtaat cattttgttg aagtctgtgt ggatgaacat aaggatgtgt ggattatgtt
     5101 gcataccggc agccgaggta ttggcaatgc catagggcaa tattttattg ctctggcaaa
     5161 aaaggatatg ggccgccaca tgcataatct gccggataag gatttagctt atctcaccga
     5221 gggcagcgag cattttaatg actatgtggc cgcagttaat tgggcgcaaa attatgcgcg
     5281 tattaaccgc gatgaaatta tggcgctggt ggtgcgggct ataaaaccgt tgttgccgcc
     5341 gtttaaaacc gaccgcgtcg ccattaattg ccaccacaat tatgtgcagc gggaatttca
     5401 ttttggtgcg gatgtatggg tgacgcgcaa gggtgct
//
LOCUS       DAOQGS010000263         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7647_length_5436_cov_3.881992, whole genome shotgun sequence.
ACCESSION   DAOQGS010000263 DAOQGS010000000
VERSION     DAOQGS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7647_length_5436_cov_3.881992"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1187)
                     /gene="pheA"
                     /locus_tag="PKZ52_10610"
     CDS             complement(57..1187)
                     /gene="pheA"
                     /locus_tag="PKZ52_10610"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNG60266.1"
                     /translation="MSGETTQDVNAAADEQAQLAQVRERIDLIDEQIGELISQRARCA
                     LEVAEIKKQFNGGKPASFYRPEREAQVLRKAMARNPGPLNNEEFARLFREIMSACLAL
                     ENPIKVAFLGPEGTFTQQAALKHFGHSAQSVPLNAIDEVFREVEAGSCQFGVVPVENS
                     TEGVVTHTLDNFMDSNLTICGDVVLRIHQNLMVSDVTKTANITRIYSHAQSLAQCRKW
                     LDAHYPNAERVALSSNSEAAKRIKSEWNAAAIAGEMAAELYGLTILADKIEDRPDNST
                     RFLIIGKLKTAPSGDDKSSIMVSMRNEPGALHGLLEPFQQHGLDLTRVESRPSRTGPW
                     NYVFFIDFTGHRDDPKVAAALAEVGKRALDLKILGSYPNGVL"
     gene            complement(1201..3810)
                     /gene="gyrA"
                     /locus_tag="PKZ52_10615"
     CDS             complement(1201..3810)
                     /gene="gyrA"
                     /locus_tag="PKZ52_10615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009470318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNG60267.1"
                     /translation="MGEIAKEVLPVSIEHEMTQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSVDGDSPAAMRYTEIRMAKIAHDLLADLDKETVDYVPNYDGTELIPVVMP
                     TRVPNLLVNGSSGIAVGMATNIPPHNLGEVIRACVALIDNPELTVDELMEYIPGPDFP
                     TAAIINGRAGILAAYRTGRGRIYVRARATVETDAKTNRETIIVHEIPYQVNKARLIEK
                     IAELVKEKKVEGISEIRDESDKDGMRIVIEIKRGEMGEVVLNNLYAQTQMQTVFGINT
                     VALVDGEPRTLNLLELLEYFIRHRREVVTRRTVYLLRKARERGHVLEGLAVAIANIDE
                     VITTIKNSQTPNDARDALLSRGWPVGLVSQFIERAGAEACRPDGLPAEMGMRDGVYYL
                     SPAQVQAILDLRLHRLTGMEHDKLLTEYEEKLKVIAEYLEILANSVRLMEVIREELVQ
                     IGADYGDARRTEIQDSLLDLTEEDLITEEDRVVTISHGGYAKSQPVSAYQAQRRGGMG
                     KAATAVKDEDFVEHLVIASTHDFILCFTNLGKVYWLKVYQIPVASRTSRGRPVVNLLP
                     LDEGERITAILPVREFDENKYIFMATSQGTVKKSSLAQFARPRSVGLRAIELDEGDHL
                     VGTSITDGSCDVLLFASNGKAARFNEEAVRETGRTSRGVRGIILAEGERVISMIIPKE
                     DGQVLTISENGYGKRTAVTEFPVKGRGTQGVIAMQISERNGVIVGAIQVFDGDEIMLI
                     SDQGTAVRTRAAEVSLVGRNTQGVRIIKTKEGERIVSLERIAESEEASADEAAFNASE
                     GEDTGTSDDSSDE"
     gene            4000..5352
                     /locus_tag="PKZ52_10620"
     CDS             4000..5352
                     /locus_tag="PKZ52_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNG60268.1"
                     /translation="MAATALPSADLILHPQWLLPIAPHNRLLERCSVAIKDGVICALA
                     PRAQIESQWQAKQVIELPQQVLMPGLVNCHNHAAMSLLSGFADDLPLMTWLNDHIWPA
                     EGQWVDEEFVTDGTTRSIAEMLLSGTTCFSDMYFFPEAAARVVNETGIRAQITFPLLD
                     FPNRWAADIDEGLRKGLSLFDDYKDHPRISIGFGPHAPYTLGDSSLRRIAILANEVQA
                     PVHIHVHETAQEVADSMAQYGQRPLARLLELGLLSPQTQCVHMTQINDEDLRLLQETG
                     AKVIHCPESNLKLASGFCPADKLLKAGIEVGLGTDGAASNNNVDMFGELHTTALLAKA
                     VAGDAAALAAEEALYMATLGGAKTLGLDSQIGSLEVGKQADMIALSLDPLLSTPSYSL
                     VSNLVYRPGSHRVNHLWVSGKALVSEGALQGLDSSQISRTTARWQQQIRGALFRPAHP
                     "
BASE COUNT         1227 a         1503 c         1437 g         1269 t
ORIGIN      
        1 gccaccgaac cgccaataag gccaagaccg ataaccacca aacgccctgg gaacatttat
       61 aaaacgccgt tggggtagga gccgagtatt tttaaatcca atgcgcgctt gcccacttcc
      121 gccagggcgg cggcaacctt tgggtcgtcc ctgtggccgg taaaatcaat aaaaaataca
      181 taattccacg ggccggtgcg ggaggggcgc gattccacgc gggttaaatc caagccgtgt
      241 tgttgaaacg gctctagtaa accgtgcagc gctccgggtt cattgcgcat ggataccatg
      301 atggaagatt tatcatcgcc actgggtgcg gttttgagtt tgccgataat taaaaagcgc
      361 gtggaattat ccggccggtc ttcaatttta tcggcaagaa tagttaggcc atacaattcc
      421 gccgccattt cgccagcaat agccgctgcg ttccattcgc ttttaatgcg tttggccgct
      481 tcagaattac tactcaatgc cacccgctca gcgtttggat aatgggcatc cagccatttg
      541 cggcactggg caagggactg ggcgtgggaa taaatgcgcg tgatattggc tgttttggtt
      601 acatccgaca ccattaaatt ttgatggata cgcaaaacca catcaccaca aatggtgagg
      661 ttggaatcca taaaattatc caatgtgtgg gtgacgacgc cttcagtaga attctccacc
      721 ggcactacac caaactggca ggagcccgcc tccacttcgc gaaagacttc gtcaatggca
      781 tttaagggta cagattgcgc cgaatggcca aaatgtttta acgccgcctg ctgggtaaat
      841 gtgccttccg gccctaaaaa agcgacttta atagggtttt ccagggctaa acaggcggac
      901 ataatttcgc ggaataaacg cgcgaattct tcattattca gcgggccagg gttgcgcgcc
      961 atggctttgc gcaacacctg ggcctcgcgc tcggggcggt aaaatgacgc gggcttgccg
     1021 ccattaaatt gttttttaat ctccgccact tcaagggcgc agcgggcgcg ctggctgatt
     1081 aattcgccga tttgttcatc gatcaaatca atgcgctcac gcacctgggc taattgagcc
     1141 tgctcatccg ctgctgcatt aacatcttgg gttgtttcgc cgctcataaa ggccgcccga
     1201 ttattcgtcg ctggagtcgt cagatgtacc tgtgtcctcg ccttcactgg cattaaaagc
     1261 cgcttcgtcc gcacttgcct cttcgctttc ggcgatgcgc tccaggctta caatacgctc
     1321 accttctttg gttttaataa tgcgcacacc ctgagtattg cggcccacca gggatacttc
     1381 tgcggcgcgg gtacgcacgg cggtgccctg gtctgagatc aacataattt catcgccatc
     1441 aaacacctga atagcgccga caattacgcc gttgcgttca gaaatttgca tggcgataac
     1501 accttgggtg ccgcgccctt taacggggaa ttcggttacc gctgtgcgtt tgccatagcc
     1561 gttttcgctg atggtaagta cttggccgtc ctctttggga ataatcatag agatcacccg
     1621 ctcaccttcg gccagaataa tgccgcgcac acctcgcgag gtacggccag tttcccgcac
     1681 cgcttcttca ttaaaacggg cagctttacc gttgctggca aacaataaaa catcgcagct
     1741 gccatcggta atagaggtgc ctaccaagtg gtcgccttcg tctaattcga tagcgcgcag
     1801 gcccacgctg cgggggcgcg caaattgcgc caatgaggat tttttcactg tgccttggga
     1861 ggtggccata aaaatatatt tattttcgtc gaattcccgc accggcagaa tagcggtaat
     1921 gcgctcaccc tcatctaagg gcaataaatt caccaccggg cggcctctgg aggtgcggct
     1981 ggccacgggg atttgataca ccttcaacca ataaactttg cctaaattgg taaaacacaa
     2041 aataaaatca tgggtactgg caataactaa atgctcaaca aaatcctcat cttttactgc
     2101 ggtggccgct ttacccatgc cccctctgcg ctgggcttga taggcactca ccggctggct
     2161 tttggcgtaa ccgccgtggg agatggtcac cacccggtct tcttcggtaa ttaaatcttc
     2221 ttcagttaaa tccagcaggg aatcttgaat ttccgtgcgg cgggcatcgc catagtcggc
     2281 gccaatttgc accagctctt cgcggattac ttccattaag cgcacggagt tggccagaat
     2341 ttctaggtac tccgcgatga cttttaattt ttcttcgtat tcggtaagga gtttatcgtg
     2401 ctccataccg gttaagcggt gcaggcgtaa atctaaaatc gcttgcactt gcgcggggga
     2461 taaataatac accccatcgc gcatacccat ttccgcaggt aagccatccg ggcggcaggc
     2521 ttcggcgccg gcgcgctcga taaattggct caccaaaccc accggccagc cgcggcttaa
     2581 taaggcatcg cgggcatcgt tgggggtttg ggagttttta atggtggtaa tcacttcgtc
     2641 gatattggcg atggcaacgg ccaggccttc caatacatgg ccgcgctcgc gagctttgcg
     2701 cagcaaatac acggtgcggc gggtgaccac ttcccggcgg tggcgaataa aatattctaa
     2761 taattccaat aaatttaagg tgcgcggttc gccgtctacc aaggctacgg tgttaatgcc
     2821 aaacacggtt tgcatttgcg tttgggcata gagattattt aataccacct cgcccatttc
     2881 accgcgttta atttcaatca cgatgcgcat accgtctttg tcggattcat cgcgaatttc
     2941 ggaaatgcct tcgacttttt tctcttttac cagctcggcg attttttcga tcaggcgcgc
     3001 tttgtttacc tggtagggga tttcgtgaac gataatggtt tcgcggttgg ttttagcatc
     3061 ggtttccacc gtggcgcggg cgcgcacata gatgcggcca cggccggtgc gataggccgc
     3121 taaaatgccg gcgcggccat taataatggc ggcggtgggg aaatccgggc cgggaatata
     3181 ttccattaat tcgtcaacgg tgagttccgg gttgtcgatt aaggccacac aagcgcgaat
     3241 aacttcacct aaattgtgcg gtggaatatt agtggccatg cccacagcaa taccggaaga
     3301 gccgttaacc aataaattgg gtacgcgggt gggcattact acgggaatta attcagtgcc
     3361 gtcgtagttg ggtacgtaat cgacggtttc tttatctaaa tcggccaata aatcgtgggc
     3421 gattttggcc atacgaattt cggtgtaacg catggcggcg gggctgtcgc catccaccga
     3481 gccgaagttg ccctggccgt ccaccagcat ataacgcagg gaaaaaggct gggccatgcg
     3541 cacgatggtg tcgtatacgg cggagtcgcc gtgcgggtgg tatttaccga ttacatcccc
     3601 caccacacgg gcggattttt tatagggttt gttccagtca ttgcccaatt cgctcatggc
     3661 gaataacacg cggcggtgca cgggttttaa tccatcgcgc acatcgggca gggcgcggcc
     3721 tacgattacg ctcatggcgt aatccaggta ggattgggtc atctcatgtt caatactgac
     3781 aggaagaact tctttagcta tttcgcccat gggcttaggg gttccttcag aacggatatg
     3841 gctgggacaa ggcgctaaaa cgcgtaaaaa accgctgcca gcgccttaag acaagggcgc
     3901 ggatactagc acagctcggc gcgattaaga acctgcctag gccgcacaac ggccaatgca
     3961 gaggttatac tgccctcccc ttacacaacg ggttcaccta tggctgccac cgctctgccc
     4021 agtgccgatt taattcttca cccacaatgg ttgctgccga ttgcgcccca caaccgcctg
     4081 ttggagcgct gctcggtggc gataaaagac ggggttattt gtgcactggc gcccagggcg
     4141 cagattgaat cccagtggca agctaagcag gttattgaat taccccaaca ggtgctgatg
     4201 ccgggtttgg taaattgcca taaccacgcg gccatgagtt tgctttccgg ctttgccgac
     4261 gacctgccct taatgacttg gctgaacgat cacatctggc ccgctgaagg ccagtgggtg
     4321 gatgaagaat ttgtcaccga cggcaccacc cgctctattg cggagatgtt actgagcggc
     4381 accacctgtt ttagcgatat gtattttttc cccgaggccg ccgcccgtgt ggtgaatgaa
     4441 acgggcatcc gcgcacaaat taccttcccg ctgctggact tccccaaccg ctgggcggcg
     4501 gacatagatg aaggcctgcg caagggctta agcctatttg acgactacaa agaccacccc
     4561 cgcatcagca tcggctttgg cccccacgcc ccctatacac tgggggatag cagcctgcgg
     4621 cgcatcgcca ttctcgccaa tgaggtgcag gcgccggtac atatacatgt gcacgaaacc
     4681 gcccaagagg tcgccgactc catggcccaa tacggccagc ggcccttagc ccgcctgctc
     4741 gagctgggcc tgttaagccc gcaaacccag tgtgtgcaca tgacgcaaat taacgacgaa
     4801 gacctgcgcc tactgcagga gaccggcgcc aaggtaatcc actgccccga atccaactta
     4861 aaactcgcca gcggcttttg cccggcagac aagcttttaa aggcgggcat agaagtgggc
     4921 ctgggcaccg atggggccgc cagcaataac aacgtagata tgttcggcga gttgcatacc
     4981 accgctctgc tggctaaagc ggtagccggt gatgccgccg ccctcgccgc tgaggaagcc
     5041 ctgtatatgg caaccctagg cggggctaaa accctcgggc ttgatagcca aatcggcagt
     5101 ttggaagtgg gtaaacaggc cgatatgatc gccctgagct tggatccact gctgtccaca
     5161 cccagctatt cccttgtatc aaacctggtg taccggcccg gcagccaccg cgtgaatcac
     5221 ctatgggtaa gtggtaaagc cctggtcagc gagggcgctc tgcagggttt ggatagcagc
     5281 cagatcagcc gcaccaccgc gcgctggcaa cagcagattc gcggcgcttt attccgcccc
     5341 gcccacccct aatgaggact tgccatggcc aatatggacc cccaggaaat tgccaaattt
     5401 agccgcctcg ccagccgctg gtgggaccca gcaggg
//
LOCUS       DAOQGS010000264         5428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7665_length_5428_cov_4.678950, whole genome shotgun sequence.
ACCESSION   DAOQGS010000264 DAOQGS010000000
VERSION     DAOQGS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7665_length_5428_cov_4.678950"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKZ52_10625"
     CDS             <1..217
                     /locus_tag="PKZ52_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60269.1"
                     /translation="KMLLHRINDKEREHRNPQQLNSTYRNRNPTKRRAARVYLLSLTP
                     GLKMRQESETATAKAGHSAFYLYRMRC"
     gene            complement(726..1724)
                     /gene="hemH"
                     /locus_tag="PKZ52_10630"
     CDS             complement(726..1724)
                     /gene="hemH"
                     /locus_tag="PKZ52_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNG60270.1"
                     /translation="MTQKRSAIVLLNLGTPDAPTVSSVRKFLRAFLSDPRVVELPRLL
                     WLPILYGIILPLRPKKALQAYQLVWTEEGSPLKAITDKQVDALAKRAQAQMGDAVEVV
                     AAMTYGEPALEAQINALRARGVGRIILLPLYPQYSATTTAPIYDQLARYIRRTRHIAE
                     VFLVNAYFERRDYIAALAASIKEYWQLNGQNEKLLFSFHGIPQRCVDKGDPYYDHCVA
                     TANAVAQALALPASAWAISFQSRLGKAKWLQPYTDKLLVEWAQAGVQSVDVICPAFAT
                     DCIETLEEIQGENQELFLHAGGKRFGYIPCLNDRADHIDMMWAIAKPLLLNSADYC"
     gene            complement(1816..2466)
                     /gene="adk"
                     /locus_tag="PKZ52_10635"
     CDS             complement(1816..2466)
                     /gene="adk"
                     /locus_tag="PKZ52_10635"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNG60271.1"
                     /translation="MRIILLGAPGAGKGTQAKHIMELFAIPQISTGDMLRNAIKEGTE
                     LGLKVKDVMASGGLVSDQLIIDLVKERIAQDDCKNGFLFDGFPRTIPQAQALADANIP
                     IDHVVDFHVDDEEIVARLSGRRVHADSGRIYHVQHNPPKQEGLDDITGEALIQRPDDE
                     EATVRKRLAVYHEQTEPLIDFYRDFSKANGGIPKCSRVNGVGPLDEIRQRVADVLK"
     gene            complement(2776..3585)
                     /gene="mazG"
                     /locus_tag="PKZ52_10640"
     CDS             complement(2776..3585)
                     /gene="mazG"
                     /locus_tag="PKZ52_10640"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNG60272.1"
                     /translation="MPHYTLADLIQLMARLRDPADGCPWDLKQSFSSITKHTLEEVYE
                     VIDAIERQDLPQLRDELGDLLFQIVFYAQLAQEQGAFDFAAVVDGITQKLLRRHPHVF
                     PDGSLASRRQSAELTDAAIKAQWEQIKSQERGEKGQPQVLADIPAALPGLIRAEKLQK
                     RVAVLGMDFADARAAIAGVKAEINELEAALATQQVDAVADELGDVLFSLVNVSRHLKL
                     DAEQCLRAANSKFSRRVVAMDKLIAERRAHWDDFDSEAFAVLWQQAKTVSD"
     gene            complement(3819..>5428)
                     /locus_tag="PKZ52_10645"
     CDS             complement(3819..>5428)
                     /locus_tag="PKZ52_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNG60273.1"
                     /translation="EDLSFRYLQPYDYKYIAKLLDERRLDRQVYIDEVISILKSALEH
                     ERIQVLDINGRAKHIYSIWRKMKRKGIGFSEVYDIRAVRILVSSEQDCYRALGIVHSL
                     WRNIPNEFDDYIANPKENGYRSLHTAVKGPASRVLEIQIRTRDMHEEAEYGVCAHWRY
                     KKSDKDGASNSYEQKIAWLRQVLEWHDEIGGNPLDDHLHRGFEQDRIYVFTPEGHVVD
                     LPENATPVDFAFRIHSDIGIRCKGAKVNGRIVPLNYSLRTADQVEIITGNREAPSRDW
                     LVPALGYITTSRARSRLQGWFRQQDRDQNIADGRALLDHEFLRLAVDNIDYEALAKKL
                     KLRTLDDLYAAVGTGDLSVDRIINVAERLFKPEKNNELVTSLVGRAQSTEPDGSDIYI
                     DGVGNLMSHIAQCCKPIPGDRITGYITLGRGVSIHRQDCTNILHLAAEEPNRIIKVDW
                     AQKPEQLYSVDVIVEAFDRHGLLRDITTLLDKERINVSAMQTLSNKQKNTVDMSFRVE
                     VASYADLSRVLARLSRLPNISSVKRRI"
BASE COUNT         1406 a         1407 c         1290 g         1325 t
ORIGIN      
        1 gaaaatgcta ctgcatagga ttaacgataa agaacgcgag caccggaatc cacagcaact
       61 aaacagcacc tatagaaacc gcaacccgac gaaacgccgc gctgcaagag tttatttact
      121 atcgttgacg ccaggtttaa agatgcggca ggaatcagaa acggcaactg ccaaggcggg
      181 gcactcagct ttctatttat ataggatgcg ctgttaagcc tgagttaata catttgaata
      241 cacacgcact gcctgttgtt caggcgtagg cttttcccac aacacgtgca aaacatccct
      301 gccttcagtc tcatcaaagg gtaactattc agcacgctat attaccccct tccgcttgag
      361 ggcgaatttt agaggattta accaggcggc ataattgatt cgcggtgtag cagtggcgat
      421 gtatcggggg ctcacaacac caaagcactc gccagatagg ttcctgtcag tgcgtgatta
      481 agtctagcct tactaaaaag tgagccaaat agttacagca aaggttccta aaatacttgg
      541 tacaaggcaa tttttgctac agcaagtaat catgatgaaa ttgagaaaaa tatcccaacg
      601 tggcgaagtg aagcgcatcc cccaataaac agcgcctgtg aacacagata ttcattacta
      661 tcgagcgggg cgattatacg agggagaaaa agcgcccagc cgggcgcaat atgcgcggat
      721 aacgattaac agtaatccgc agaattgagc aataagggtt ttgcgatggc ccacatcatg
      781 tcgatatgat ctgcgcggtc attaagacat ggaatatacc cgaagcgttt gccgcccgcg
      841 tgcaaaaaca actcttggtt ttccccttgg atttcttcca gggtttcaat gcaatccgtt
      901 gcgaatgcgg ggcagataac atccactgac tgcacaccgg cctgcgccca ttcaaccaac
      961 aatttatcgg tatagggttg tagccatttg gctttgccta agcgtgattg aaaactaatc
     1021 gcccacgcag aagcaggcaa tgcaagcgcc tgtgcaactg cattggccgt ggcaacacag
     1081 tggtcgtagt acggatcgcc tttatcgaca cagcgctgcg gaatgccgtg aaaagaaaat
     1141 aatagttttt cgttctggcc attgagctgc caatactcct taatacttgc cgccagtgcc
     1201 gcaatataat cccgccgctc aaaataggca ttaaccaaaa acacttccgc aatatggcgc
     1261 gtgcgcctga tatagcgagc cagttgatcg tatatcggcg cggtggtggt agctgaatat
     1321 tgcggataca ggggcaatag aataatgcgg ccaacgccac gtgcccgtag cgcatttatt
     1381 tgcgcttcca gtgctggctc accataggtc atggccgcca ccacttcaac cgcatccccc
     1441 atttgagcct gcgcgcgttt agcgagggca tcgacttgtt tgtcagttat tgccttcaac
     1501 ggcgaacctt cttcagtcca caccaactga taggcttgca gagctttttt cggcctcagg
     1561 ggtaaaataa tgccgtataa aataggcagc cacagcagcc tgggcaattc caccacgcgc
     1621 ggatcgctca aaaatgcgcg cagaaacttt ctcacactgg aaactgtcgg cgcatccggc
     1681 gtgcctaaat taagtaaaac aattgctgat cttttctgag tcacactgta ttcccgctaa
     1741 tttgaaggcg aaaaaaaagg ctctaaagag ccaattttag caatagtgcc gcaccgagcg
     1801 acgatgggca taggcctact tcaatacgtc cgcaacacgc tggcggatct catcaagcgg
     1861 ccccacgcca tttactctgg aacacttagg aataccgcca ttggctttcg agaaatcgcg
     1921 ataaaaatca attagaggct cagtttgttc atgatataca gccaggcgtt tgcgcaccgt
     1981 ggcctcttca tcatctgggc gctgaataag cgcttcaccc gtaatatcat caagcccctc
     2041 ttgcttgggc ggattgtgtt ggacgtggta aatgcgcccg gaatcggcat ggacacggcg
     2101 gccacttaaa cgcgccacga tttcttcgtc gtcaacgtga aaatcaacta cgtgatcgat
     2161 gggaatattg gcgtcggcta aggcctgggc ttgcgggatg gtgcggggaa atccatcgaa
     2221 taaaaaaccg tttttacaat catcctgcgc gatgcgctct ttaaccaaat caataattaa
     2281 ttggtccgat accaatccgc ccgatgccat tacatctttg actttaagcc ccaactcggt
     2341 gccttcttta attgcatttc tgagcatatc gccggttgaa atttgcggaa tagcaaacaa
     2401 ctccatgatg tgtttagctt gggtcccttt tccggcgccg ggagcaccta aaagaataat
     2461 ccgcatgaat gagttctccg ataaataagg ccgcaggcct gggcataaat aaggccgtag
     2521 gcctggacaa agcggctaag atacacgcag ggccaaactt tcacaaggtt agggcccgca
     2581 gggccaggca gctggttaat ttccaagcgg cgcacgatca catactgtac gcgcaaggcc
     2641 caatttatag cagcttttat atggctgatg tgtagctttg tgagctgtga gaagaaccgc
     2701 tgaaggggct gctgaattta ttagggaggc cgtgacaatc taagtattct aacccttatt
     2761 gcttcccatc atttattagt cgctgacagt tttggcctgc tgccaaagca ctgcgaatgc
     2821 ctcgctatca aaatcgtccc agtgcgcgcg ccgctcggca attaatttat ccatggccac
     2881 tacccggcgc gagaatttac tattagccgc ccgcaaacac tgttcagcat ctaatttcaa
     2941 atggcgcgag acattcacca aactaaataa tacatctccc aactcatcgg caactgcgtc
     3001 cacctgctgg gttgccagcg cagcttcaag ctcgttaatt tccgctttta cgcctgcaat
     3061 cgcagctctg gcatcagcaa aatccatccc cagcacagcc acacgttttt gcaatttttc
     3121 cgcgcggatt aaacccggca gcgccgctgg aatatcggct aaaacttgcg gctgcccttt
     3181 ttcgccgcgc tcttgagatt tgatctgttc ccactgagcc tttattgccg cgtcggttaa
     3241 ttcagcactt tgccgccgac tggccaagct gccatcggga aatacatggg gatggcggcg
     3301 caacaatttt tgtgtaatac catccacaac agccgcgaaa tcaaatgccc cctgctcttg
     3361 tgccaactgc gcataaaaca caatttgaaa taataaatcc cccaactcgt cgcgcagctg
     3421 cggcaagtcc tggcgctcga tggcatcgat cacctcatac acttcttcca acgtatgctt
     3481 ggtaatactc gaaaaacttt gttttaaatc ccacgggcaa ccatctgctg ggtcgcgcaa
     3541 gcgggccata agttggatta aatcggcgag tgtatagtgg ggcatgtcaa aagcctgtgt
     3601 tgaggatgct ctgattcagc ttgcaagtgt aagtattatt aacccggaag agtttttctg
     3661 ccgggccaat ggttacagcg caagccgccg cgagctgcgc taaaacatga acatccacgc
     3721 gtgcttacag gttttagcgc agcgagaatt taaagaaggc agctaaagca gatatttctt
     3781 tgccgcctat tttcacgcta tcgcacctgg gcggggcgtt aaatacggcg ttttacactg
     3841 gagatattcg gcaagcggct taagcgcgcc aatactcggc tgaggtcggc ataactggcc
     3901 acctcaacgc gaaaggacat atccacggta tttttttgtt tattggagag tgtttgcatg
     3961 gcgctgacgt tgatacgttc tttatccagc aacgtggtta tatcgcgcaa taagccatgg
     4021 cggtcgaagg cttccactat gacgtctacc gaatacaact gctcgggttt ttgcgcccaa
     4081 tccactttaa taatgcggtt gggctcttct gcggccaaat gcagaatatt ggtgcaatcc
     4141 tgccggtgaa tggaaacccc gcggccaagg gtgatataac ccgtgatacg atcccccgga
     4201 atgggtttgc agcactgcgc tatgtgggac attaaattgc ccacaccatc gatataaata
     4261 tcggagccat ccggctcggt cgattgcgca cgccccacca aggaggtaac taattcgttg
     4321 tttttttccg gtttaaataa gcgctcggca acattaataa tgcggtcaac acttaaatca
     4381 cccgtgccca cagcggcata gagatcatcc aaggtgcgca atttgagttt tttcgccagg
     4441 gcctcatagt cgatattatc caccgccaag cgcaaaaatt cgtgatccag tagggcgcgg
     4501 ccatcggcaa tattttgatc gcgatcctgc tgccgaaacc acccctgcaa acggctgcgc
     4561 gctcgcgagg tggtgatata acccaacgcg ggcactaacc aatcccggct gggcgcttcg
     4621 cgattgcccg taataatttc tacttgatcg gcggtgcgca aggaataatt aagcggcact
     4681 atgcggccat tgactttcgc gcctttgcag cgaataccta tatccgagtg gatacgaaaa
     4741 gcaaaatcca ccggcgtggc attttccggc aaatccacca catggccttc cggcgtgaag
     4801 acataaatac ggtcctgctc aaaaccgcga tgcaaatgat catccagcgg attgccgcca
     4861 atttcatcgt gccactcaag cacttggcgc agccaagcaa ttttttgttc gtaggaattg
     4921 gacgcgccgt ctttatcgga ctttttgtag cgccagtggg cgcagacacc gtactcggct
     4981 tcttcgtgca tatcgcgggt gcgaatttga atttccaaca cgcggctggc gggccctttt
     5041 acagcagtat gtagtgagcg gtaaccattt tctttggggt tggcgatgta atcatcgaac
     5101 tcattgggaa tattgcgcca gagcgagtgc actataccta gagcgcgata acaatcctgc
     5161 tcggaggaga ctaaaatgcg cacggcgcga atatcatata cttctgaaaa gccaatgccc
     5221 ttgcgcttca ttttgcgcca gatactgtaa atatgtttgg cgcggccatt gatgtcgagc
     5281 acctgaatac gctcgtgctc caaagcactt tttaaaatgc ttataacttc atctatatat
     5341 acctgacgat caagcctgcg ctcgtctaat aatttagcga tgtatttata atcgtagggt
     5401 tgtaaataac ggaacgataa gtcttcca
//
LOCUS       DAOQGS010000265         5398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7734_length_5398_cov_4.104810, whole genome shotgun sequence.
ACCESSION   DAOQGS010000265 DAOQGS010000000
VERSION     DAOQGS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_5398_cov_4.104810"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..1887
                     /locus_tag="PKZ52_10650"
     CDS             412..1887
                     /locus_tag="PKZ52_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60274.1"
                     /translation="MRIKLIHKLLGINIGIILVLTVVFVSLSFVGSKSMYSNALNGID
                     LAVMEDLAATLSQEYAQNNSWDKVIADKSLWNDTVNHSFYKVFFALMAQNAATSLNKE
                     QRAAPIPAPPPKSTPAWDMPFGTFLQRATLLDANKRVLIAPEIAKSDVNYQAIKLHGK
                     LIGWLSIGKIDMDILPLAQYFFYQQLYIVNWAIALGGILAAVLSFILSRHITRPIQEL
                     TRGAQEIAQRNFATRINIQSNDELQELAERFNSIATELASYHRQQKQWLTDISHELKT
                     PLTLLVGEIFAICDNLTQCDETTAQNLQHEVLHIKRLVDDLYELSKGEEIGLILRPQT
                     LDLKPFIEYIVKPYKTLFREKDIQLEEHYSAEAFYILADADRLTQVVRNLLENCLKYM
                     DSPGRLNIQLMEKPQAVCISLDDSGPGVTPAAAKHMFDRLYRADTITGRSGGGAGIGL
                     AICKSIIDAHGGAIFATTSELGGLSIRIELPTRAPKTLEHE"
     gene            1880..2554
                     /locus_tag="PKZ52_10655"
     CDS             1880..2554
                     /locus_tag="PKZ52_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG60275.1"
                     /translation="MNKTILVAEDDTKIADIICKYLSLEDFSVRHVRTGLEALAAFNT
                     TPPDLIILDVMLPELTGTEVCEHIRAQSNVPIIMISALAEDAHRLAGFAHGTDDYLCK
                     PFNPRELIARIKAILRRAGPQAAEVHTISHGPICLHPEEQRVTVAGEPVLLTQIEFNM
                     LLLLISSPTKVFSRESLLDKSHKHYAEYDARSVDFHIKNLRRKINIQPDVQYIKSVYG
                     AGYKLI"
     gene            2882..4081
                     /locus_tag="PKZ52_10660"
     CDS             2882..4081
                     /locus_tag="PKZ52_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG60276.1"
                     /translation="MARKAIFTLFLSVLSLLIGCGQHTAPTEIARPVRTLVVAANQQQ
                     LAAEYAGTIVARVESALAFQVGGKIQARHVEVGSQVKTGQLLMSLDNQDLKLGLTQAQ
                     ANARTAQTQFDVAALEYKRFSDLLKTGAISHSAVDAKNVAFAAAKAYLEQAQAIEREQ
                     ANQTRYAQLIADVDGVVTALHGEIGQVVAPGVPVVQLAKLSGDNTHAGPSDLELAIAI
                     PENNLGLINKADAIDIQLWASTKEVFHGRIREIAPAADPISRAFAAKISFIDMSDAQR
                     ALLKLGMSAAAKFHLTTPQAFIKLPLSALLNNQGKTQVWQVVQGRAELTEIKVAGISG
                     NDVLVEGLHPGQTIITAGVHVLTSGQAVSLLPAPAQTQQTPARLSAQALMAPAAEHTQ
                     SNGAVSQ"
     gene            4078..>5398
                     /locus_tag="PKZ52_10665"
     CDS             4078..>5398
                     /locus_tag="PKZ52_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG60277.1"
                     /translation="MNSSFNLSRWALEHIPLVRYLIAALLIGGILSFVNLGQDEDPPF
                     TFRAMVVSAKWPGATAMQMADQVADKLEKTLQETPYVDSIRSFSRPGETTIIIQLAES
                     TPPKETSNSWYHVRKKMGDIRYSLPQGVLGPFFNDQFGDTYGSIFALSADGFSYAELK
                     DYADLVRQELLRVPQVAKVELLGVQDEKINVVLSHKKMARMGISLPEIIERINAQNAL
                     TASGVLATETDNINLRVSGQLASLDELRALSLGREGRDLRLGDIAEITRGYDDPPKNK
                     MRHNGEEVIGLAVAMQKGGNIIELGKNLNHTGEIIRHKLPAGIELHRVADQPKAVASS
                     VNEFLGTLAEALIIVLAVSFVALGVHKTPTGWAVSLRPGLVVGLTIPLVLAITFLCMR
                     LFNIDLHKISLGALIIALGLLVDDAIIAAEMMVRKMEEGFSRIEAATF"
BASE COUNT         1409 a         1399 c         1314 g         1276 t
ORIGIN      
        1 agctggaaga agagctgctt gagctagatg aagtcgtggc gccaccgcag ccagaaacgg
       61 ccgccaatgc cagcagtata ctgcccaaaa cagctgtttt ttttaaacgt gatttctcta
      121 tgacaggcat ggttatacac cccattatga attatggttg ttattagatt gtgtaaaaaa
      181 tagaaacggg gcacggcatg gcgcaagcgc gccagcggtc acaaataaac aagcacttaa
      241 aagcttgtgt tgctttatga gactatatcg agtaattgtg aggaacctgt gaggctgcca
      301 ctgccaacac cagcaaacga gagcttcggc acgttttaag tgtgatcgag attgaatcct
      361 aacactctat ttttaccctg gcggccatac aagccttttg cgggatgtac catgcgcatt
      421 aaattaatcc acaaactact cggcattaat atcggcatta ttctggtgct caccgtggtt
      481 tttgtcagcc tgagttttgt cggcagcaaa agcatgtaca gcaatgcact caatggaatt
      541 gatttggcgg taatggagga tctggcggca actttaagcc aagaatacgc acagaataat
      601 tcctgggaca aggtcatcgc cgataaatcc ctctggaatg acacggttaa tcacagtttt
      661 tataaagtat tttttgcatt gatggcgcaa aatgccgcca cctcgttaaa taaagaacag
      721 cgcgcagcgc ccattccagc gccgccacct aaatctaccc ctgcctggga tatgccattt
      781 ggcacttttt tacaaagagc cacactatta gacgccaata aacgcgtcct gattgcaccg
      841 gaaatagcta aaagcgatgt taattaccaa gccattaaat tacacggcaa actgattggc
      901 tggctgagca ttggcaaaat tgatatggat atattgccac tggcccagta ttttttttat
      961 caacagctgt acattgtcaa ttgggctata gcattaggcg gtattttagc cgcggtgtta
     1021 agttttatat tgtcccgcca tattacccgc cctattcaag agctcacccg cggcgcccaa
     1081 gaaattgccc agcgtaattt tgccactcga attaacattc agtccaatga tgaactacag
     1141 gagttggctg agcgctttaa tagcatcgcc actgaattag ccagttacca caggcaacaa
     1201 aagcagtggt taacggatat ttcccatgaa cttaaaactc cgctgacact tttggtgggg
     1261 gagatttttg ccatttgcga taatttaacc cagtgcgatg aaaccaccgc gcaaaattta
     1321 cagcatgaag tactgcatat taagcgcctg gtagatgatt tgtatgagtt gagtaaaggt
     1381 gaagaaattg gcttgatctt aaggccccaa accctagact taaaaccctt tattgagtac
     1441 atagtaaagc cctataaaac actctttcgc gaaaaagata ttcaactgga agagcactac
     1501 agcgcggaag cgttttatat tctggccgat gccgaccggc tcacccaagt ggtgcgcaac
     1561 ctgttagaga attgtttaaa atacatggac tcaccgggcc ggcttaacat tcagctgatg
     1621 gagaagcccc aggccgtctg catcagcctg gatgattccg gccccggggt aacccctgct
     1681 gcggctaaac atatgtttga tcgcctctac cgcgccgaca ccatcacagg ccgcagcggc
     1741 ggtggtgcag gcattggcct tgccatttgc aaatccatca tcgatgccca cggcggcgct
     1801 attttcgcaa ccaccagcga attgggtggt ttatcgatta gaatagagct gcccacccgc
     1861 gcaccgaaga cattagagca tgaataaaac gattttagtg gcagaagacg acaccaaaat
     1921 tgccgatatt atctgcaagt atttatccct ggaggacttt agcgtgcgcc atgtgcgcac
     1981 ggggcttgag gcactggcgg catttaatac aaccccgccg gatttaatta ttctcgatgt
     2041 catgctgccg gaattaaccg gcaccgaagt atgcgaacac attcgcgccc aatcgaacgt
     2101 gccgattatt atgatcagtg cactggcaga agatgctcac cgcctggcag gctttgccca
     2161 cggcaccgat gattatttgt gtaaaccgtt taatccccgt gaattaattg cacgcatcaa
     2221 ggctattttg cgccgcgcag gcccccaggc ggccgaggtg cacaccatca gccacggccc
     2281 catttgcctg cacccagaag aacagcgcgt gacagttgcg ggcgagcccg tgttattaac
     2341 gcaaattgaa tttaatatgc tgctgttatt aatatccagc ccgacaaaag tattttcccg
     2401 cgaaagcctc ttggataaaa gccataaaca ctacgctgaa tacgacgctc gctcggtgga
     2461 ttttcacatt aaaaacctgc gccgcaaaat taatattcag ccagatgtgc aatatattaa
     2521 atctgtgtat ggcgcaggct acaaactcat ttaagcgctt gttgcctatc tatcttcagc
     2581 aaaccattaa gcaaattctg cgcaccgcta aattcgcgcc tattgtgcaa atgcaccggg
     2641 tgcagcgtcc ccgcctcaag gcttaatccg aggttcctta ataaatcgtc ttaacgctgg
     2701 cctgccacta cgcagccctg cacgcaacct agcccgctag gcaatttgtc acacccaggc
     2761 ggggtaaaag taaatttccg cgctgcatcg aaaggggtaa actgcacagg tttatcttag
     2821 ggcccatcgg cacagacgat tgggctcccg cttcaattgc tgacaacctc taggaaccgc
     2881 tatggcgcgc aaggctattt ttaccctatt tttgagtgtg ttaagcctgc tgataggctg
     2941 cgggcaacac accgccccca ctgaaattgc gcgcccagtg cgcaccctgg tggtcgccgc
     3001 caaccagcaa cagctggccg ccgaatacgc aggcaccatt gttgcccggg tggaatcggc
     3061 cctggccttt caagtgggcg gcaaaattca agcgcgccat gtggaagtgg gcagccaggt
     3121 aaaaaccggc caattgctca tgagtttgga taatcaagat ttaaaactcg gccttactca
     3181 agcccaagcc aatgcgcgca ccgcgcaaac tcagttcgat gtggccgcac tagaatataa
     3241 gcgttttagc gatttactta aaaccggcgc catcagccac agcgctgtgg atgctaaaaa
     3301 cgtagcgttt gccgctgcca aagcctattt agaacaggcc caagccattg agcgtgagca
     3361 ggccaatcaa acccgttacg cgcaattaat tgccgatgtg gacggtgtag tcaccgccct
     3421 gcacggcgaa attggccaag tggtggcgcc gggtgtaccg gttgtacaac tggctaaact
     3481 cagtggcgat aatacacatg ctgggccgag cgatttggaa ttggccatcg ccattccgga
     3541 gaataatctc ggcttaatta ataaagccga cgccatcgac attcaattat gggcatcgac
     3601 taaagaagtt tttcacggcc gcatacggga aattgcccct gccgccgatc ctattagccg
     3661 cgcctttgca gcgaaaatat cttttatcga tatgtcagat gcccagcgtg cgctactaaa
     3721 attaggtatg agcgcggcgg caaaatttca cctcaccaca ccgcaagcct ttataaaact
     3781 gccgctctct gcactgctca acaaccaggg taaaacccag gtgtggcaag tggtgcaggg
     3841 ccgtgctgag cttaccgaaa taaaagtggc gggcatcagc ggcaacgatg tcttagtgga
     3901 aggccttcac cccggccaaa cgattattac ggcaggcgta catgtgctca cctctggcca
     3961 ggccgtaagc ctgctgcccg ccccggcgca aacacagcaa acgcccgcac gtctgtcggc
     4021 acaggcgctg atggcgccgg ctgctgaaca cactcaatcc aacggagccg tatcccagtg
     4081 aatagctcat ttaatttatc tcgctgggcg ttagaacata ttccactggt gcgttattta
     4141 atagccgcac tattaattgg cggcatttta agttttgtaa atctcggcca ggatgaagac
     4201 ccgcccttta cctttcgcgc catggtggtg tcggccaaat ggcccggcgc caccgccatg
     4261 caaatggccg atcaagtggc cgacaaactg gaaaaaaccc tgcaggaaac gccatatgtg
     4321 gacagcatcc gcagtttttc caggcccggc gaaaccacca ttattatcca attagccgaa
     4381 tccacaccgc caaaagaaac cagcaacagc tggtaccatg tgcgcaaaaa aatgggcgat
     4441 attcgctata gcttgccgca aggtgtttta gggccgtttt ttaacgatca attcggcgat
     4501 acctacggct ctatttttgc gctctcagcc gatggtttta gctacgcaga attaaaagac
     4561 tatgccgatt tagtgcggca agaattgctg cgcgtacccc aagtggccaa agtcgaatta
     4621 ctcggcgtgc aggatgaaaa aattaatgtt gtgctctccc ataaaaaaat ggcgcggatg
     4681 ggcatttcgc tgccggagat tatcgagcgc attaacgccc aaaatgccct tactgcaagc
     4741 ggtgtgctcg ccaccgagac cgataatatt aatctgcggg tgagcgggca attggcctcc
     4801 ctagatgaac tgcgcgccct cagcctcggc cgcgagggca gggatttgcg cctgggcgat
     4861 attgccgaga ttacccgcgg ctatgacgac ccgccaaaga ataaaatgcg ccacaacggc
     4921 gaggaggtta ttggcctggc ggtggccatg caaaaaggcg gcaatattat tgagctaggc
     4981 aaaaatctta accacacagg ggaaattatt cgccacaaat tgcccgccgg cattgaatta
     5041 caccgggtgg cagatcagcc caaggccgtg gccagctccg tcaatgaatt tttaggcacc
     5101 cttgccgaag ctttaattat tgtgcttgcg gtaagttttg ttgccctggg cgtgcacaaa
     5161 acgcctaccg gctgggcggt aagcctgcgg ccgggtttgg tggtggggct gaccattcct
     5221 ttggtgctgg ccattacttt tttatgcatg cgtttattta atatcgattt acacaaaata
     5281 tccctcggcg cgttaattat tgccctgggg cttttggtag atgacgccat tattgcggcg
     5341 gagatgatgg tgcgcaaaat ggaggaaggg ttttcccgca tagaagcggc cacctttg
//
LOCUS       DAOQGS010000266         5398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7735_length_5398_cov_3.983904, whole genome shotgun sequence.
ACCESSION   DAOQGS010000266 DAOQGS010000000
VERSION     DAOQGS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7735_length_5398_cov_3.983904"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKZ52_10670"
     CDS             complement(<1..491)
                     /locus_tag="PKZ52_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG60278.1"
                     /translation="MRIIIVALGILLAAYATAADELRVRYPSIQHPYFAKRDVYFVTL
                     LRMALERSGEPFKLINVDFSEYSERRSVLLLQSDQYDVHWMNTTAEREHELLPVRIPL
                     CKGVIGWRAFFIRPEDQARFDKINVINDLRPFIFVQGHDWADSDILQKNGLVPIFSSF
                     LLLL"
     gene            1015..3375
                     /locus_tag="PKZ52_10675"
     CDS             1015..3375
                     /locus_tag="PKZ52_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60279.1"
                     /translation="MVRVKTTSLAARLVQVVLAIYLSLAIVLTIGQLALEYQNEKQRF
                     TQEVENIATTFAPIIAKSLWNVDEEQTRVTLQGVLGINYDVLSVKLLDANARVLHEFA
                     PTTEKHRLFSDWPLLHQLTKPFLEEYVFKYDLFYTGGFTSNQKIGALLLTSNSDVILS
                     RAAHTFFITMVSAVFKTTLLSFIFYFILRIMVGKPLMRITRVMQSLTADESAQPPAVD
                     VDLLARSDELGGMARTFNEMTSALKRKDEALNTYSLHLEAKVQERTLQLERASQAKSE
                     FLAAVSHEIRTPMNGVIGLAHLLAETELNPQQRQYVTTIEQSGETLTKLINEILDHLK
                     LESNKIELDITPIQPEALLHESAALFLRQARQANIEVVITYAPECPQSILGDGVRLRQ
                     VLVNLLGNAFKFTQQGRIVVQGKLLAARAEQAAPALLFSIQDTGIGIEAAQIQRLFKP
                     FSQADSSTTRRYGGTGLGLAICKQLVELMGGSIGVESRPGEGSTFWFTVPVQCVEDSA
                     PAVEIMQAPAGVYLTPDGSFYEEHFIGLLHYHNIPLQRLPNRSALLNALPAQTQQQVV
                     IVMAHCIQQSADNENLADFIQRLREVSDIPVLIVAPEYCAQFAAHLDAAMRLLVTPCS
                     NKAFYQALTQLALGISWAKEGRAPAKTYTNFSALKVLVAEDNPVNQMVITGLLKKYAI
                     EPVLVENGRAAVDYCSAHPQAVDLILMDGEMPVLDGWQAALQIRALNMRRTNGAPIMI
                     TAMTAHVFDQAADSLSRYGMDNILSKPTKPAELEAILRDCDNRGQA"
     gene            3673..4266
                     /locus_tag="PKZ52_10680"
     CDS             3673..4266
                     /locus_tag="PKZ52_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60280.1"
                     /translation="MSENIYTPPKAEPLPSHQQANQPYFYVVANKKCMVLFLGTVGMY
                     MVYWMYKNWSLFKAATGESVMPVMRGIFSVFFTHSLFNAVQTKLEEDSRKVEWDPNAL
                     ATGLVILVVLSNVLSMVGNRVFHVSILDYVSFVLLIPIGLQMLKAQRAINIACGDEAG
                     ASNDRFTTANILWLLFGALFWVLIIIGIFVEPPAATV"
     gene            complement(4352..>5398)
                     /locus_tag="PKZ52_10685"
     CDS             complement(4352..>5398)
                     /locus_tag="PKZ52_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60281.1"
                     /translation="LHAKLESGEPVLLEDNRHLLINYFLPKQKAVLTLAVASPASSNE
                     QASITLTLVFYCGLILILVLCLYPLVRRLMLLQESARAFGAGDLNARLAPSRWSYIRS
                     LEEAFNQMAERVQSLLADNRLLSRAVSHDLKTPMARLRFGFAMLEEAQTAEQSTRYLQ
                     RIEQDLSAMEALINRLLEYAKLEEGQIKLDLQPLNLNSLVARLWEHVRDDEHSLELAL
                     APEELWVQADSHYLAMLINNLLSNALRYCRTRVCLRTARHKHSVVLSLSDDGPGIPRE
                     EHRQVFKPFVRGQKVNSSGQGPAEGHGMGLAIVERIATWHKAQIHLDTDAALGGLCVQ
                     LRLAAIEFPAGATE"
BASE COUNT         1312 a         1257 c         1450 g         1379 t
ORIGIN      
        1 agcaacagca aaaatgagga aaatatgggg actaaaccat ttttttgcaa aatgtcagaa
       61 tccgcccagt cgtggccctg cacaaaaata aacgggcgca gatcattgat gacattaatt
      121 ttatcaaacc gcgcttgatc ttccgggcga ataaaaaagg cccgccaacc gataacgcct
      181 ttacacaggg ggatgcgcac gggcaataat tcgtgctccc gttcggcggt ggtgttcatc
      241 cagtgcacat cgtattggtc ggattgtagc agcagaacac tgcgccgctc gctgtattca
      301 gagaaatcca cattaattaa tttaaatggc tcccctgagc gctccagcgc catgcgcagg
      361 agggtgacaa aatacacatc ccgtttagcg aaataggggt gttgaatcga tggatagcgc
      421 acgcgcagtt catcagcggc tgttgcataa gccgccaata atatgccgag cgcaacaata
      481 atgatgcgca tgatgttagc ccttaaatac ttgtgttatc gctattctga ggcgctttta
      541 gctggctatt gaaaagtggt aaatggtatt tcaacagatg gccatgtgca ttttcagttg
      601 cgtttttatg tggtgtgcct tttttgttat tttatagcag tagttcaccc atgcctatgg
      661 ctagtttgcc tacacgcagg ggatcaaaac agtataggct agcttgccaa aatggctctt
      721 atagcatcta gccgtatggg gtggcggggt ttgggtttgc gcaagagttt taattggctg
      781 ataaaagcct ccacagcgtt tataatccca ggccataggt taacgcgggt tttgcccagc
      841 agcttagttg aaccagcagt cagccgaaca ttacatcaag tgaaaaaatc acgatcccta
      901 tagctttagc agacaggctg cccgctgggt ttggcaatca ttactgtgcg ccttgtggtg
      961 tttgctacta tttcacgacc acaaccagaa tgccgacgga ggcgaacacc acacatggta
     1021 cgagttaaaa ctacttcatt agcggcgcgc ctagtacaag tggttttagc tatttacctc
     1081 agtttggcca ttgtgttaac catcgggcag ctcgccttag aatatcaaaa tgaaaaacag
     1141 cgcttcaccc aggaggtaga aaatatcgct accacattcg cgccgattat tgccaaatcc
     1201 ctttggaatg tggatgagga gcagacgcgt gtgacgctgc agggggtgtt gggcattaat
     1261 tacgatgtgt taagcgttaa actgttggat gctaacgcgc gtgtgctgca cgaattcgcc
     1321 cccaccacgg aaaaacaccg cttgttttcc gattggccgt tactgcatca gctcaccaag
     1381 ccgtttttag aagagtatgt gtttaaatac gatctgtttt ataccggtgg ctttacaagt
     1441 aatcaaaaaa taggcgcgct gctgcttacc agtaattccg atgttatttt aagccgcgcc
     1501 gcccatacct tttttattac catggttagc gccgtattta aaacaaccct gttgtcgttt
     1561 attttttatt ttattctgcg catcatggtg ggcaaaccct taatgcgtat tacccgggta
     1621 atgcagagtt taacggcaga tgaatctgct cagccaccgg cggtggatgt ggatttgctt
     1681 gcgcgctcgg atgagctggg cggtatggcg cgcacgttta atgagatgac cagcgccctt
     1741 aagcgtaaag acgaagcgct caatacttat tcgctgcacc tcgaggcaaa agtgcaggag
     1801 cgcaccttgc aattggagcg cgcctcccaa gcgaaaagtg aatttttggc ggcggttagc
     1861 catgaaattc gcacgcctat gaatggcgtt attgggttgg cgcacttatt agctgaaacc
     1921 gagttgaacc cgcagcagcg ccaatacgtt actaccattg agcaatccgg cgagaccctc
     1981 actaaattaa ttaatgaaat attggatcat ttaaaactag agtccaataa aattgaattg
     2041 gatattacgc ctattcaacc tgaagccctt ttgcatgaaa gcgccgcgct ttttttgcgc
     2101 caggctcgcc aggcgaatat tgaagtggtt atcacctatg cgcctgagtg cccgcaatcg
     2161 attctgggag atggtgtgcg gttgcggcag gtgctggtga atttattggg caatgcgttt
     2221 aaattcaccc agcagggccg cattgtggtg cagggtaaat tacttgccgc cagggcggag
     2281 caggccgccc ctgcgttgtt attttcaatc caagataccg gtatcggcat tgaggctgcg
     2341 cagattcagc ggttatttaa gccctttagc caggcggata gttccaccac acgccgctat
     2401 ggcggcacag gtttgggcct ggctatttgt aaacaattag tggagttaat gggcggcagt
     2461 atcggtgtgg aaagccgccc aggcgaagga tcaacttttt ggtttacagt gccagtgcag
     2521 tgtgtggagg attctgcacc agcagtagaa attatgcagg cacccgcggg ggtttattta
     2581 acaccggacg gctcttttta tgaagagcat tttattggcc tattacacta ccacaatatt
     2641 ccgctacagc gcttgcccaa tcgcagcgcg ctgctcaatg cgctgcccgc ccaaacacag
     2701 cagcaggttg ttattgtgat ggcccattgc atacagcaga gtgctgataa tgaaaattta
     2761 gccgatttta tacagcgcct acgcgaggtg agtgatatac cggtgttaat tgtggcgccg
     2821 gaatattgcg cgcaattcgc agcgcattta gatgcggcta tgcgtttatt ggtaacgccc
     2881 tgtagcaata aagcttttta tcaagcgctc acgcaattag ctttaggtat cagctgggct
     2941 aaagagggta gagcgcctgc aaaaacctat accaatttta gtgcgctgaa ggtgttggtg
     3001 gcggaggaca atccggtaaa ccaaatggtt attactggtt tattaaaaaa atacgctatt
     3061 gaacctgtgt tggttgaaaa tggccgcgcg gctgtggatt actgtagcgc tcatccccag
     3121 gcggtggatt taattttaat ggatggcgaa atgcccgtgc tggatggctg gcaggctgcg
     3181 ctgcagattc gcgcgctgaa tatgcgccgc acaaatggcg cgcctattat gattacggcc
     3241 atgaccgccc acgtgttcga ccaagcggcc gatagcctca gccgatacgg catggataat
     3301 attttaagta agcctaccaa acccgccgag ttagaagcga tattgcgtga ttgtgacaac
     3361 cgtgggcagg cataaagcct gcgctaggcg agtattttgg ggtgatttag aggttcagta
     3421 caccttatta ctctcctaag tgggccaaaa attaataagt aggcggaaaa ttaaaaagga
     3481 ggcgggaaat taaaaaggat ttagccaagc ggcatgcgcg attcgcgatg tggcagtggt
     3541 gcatagctgc gtgggcttgc agcactaaaa cacacacctg atcgttttta gccggtgcga
     3601 gaatcacatc cagctgatct aaaaagtgct gaaaatcaca ccctattttt agtgttaaag
     3661 aggagttaac ccgtgagcga gaatatttac acacccccta aagccgaacc tttgccttct
     3721 caccaacagg ctaaccagcc gtatttttat gtggtggcta ataagaaatg catggtgtta
     3781 tttttgggca ccgtaggcat gtatatggtg tattggatgt ataaaaactg gagcctattt
     3841 aaagcagcta cgggggagag cgtaatgccg gttatgcggg gtattttttc ggtgtttttt
     3901 acccattcac tatttaatgc ggtgcaaact aagcttgaag aggacagccg caaagtagag
     3961 tgggacccga atgccctggc caccgggctg gtgattttag ttgtgctctc caatgtgctg
     4021 agtatggtgg gcaatagggt tttccatgtc agcattttgg attatgtttc gttcgtgctt
     4081 ttaattccca tcgggttgca aatgctcaag gcacaaaggg caattaatat tgcctgcggc
     4141 gatgaagcgg gcgcaagcaa tgatcgtttt acgacggcca atattctctg gttgttattc
     4201 ggtgcgctgt tctgggtgtt aatcataatc ggtatttttg ttgaaccacc ggcggcaact
     4261 gtttaagcct cttgtcgcga ataataatag ccctagcata tctctacggt gtgcccgcca
     4321 aagtaagttg catagattct cttctgcaga cttattcagt ggctcccgca ggaaactcta
     4381 tcgcggcaag cctaagttgc acgcataacc cacccagggc tgcatccgta tccaaatgga
     4441 tttgcgcttt atgccaggtg gcgatgcgct ccactatggc aagccccata ccgtggccct
     4501 ctgcaggccc ctggccgctg gaattaacct tttggccgcg cacaaagggt ttgaatacct
     4561 ggcggtgctc ttcacgggga atgccggggc cgtcgtcgct gaggctcaac accacggagt
     4621 gtttgtgccg ggcggtgcgc aggcagacgc gcgtgcggca gtagcgcagg gcattgctca
     4681 gcaggttgtt aatgagcatg gccaggtagt ggctgtctgc ttgtacccac agctcctccg
     4741 gggcgagggc aagctctagg ctgtgctcgt cgtcgcgcac atgctcccag aggcgcgcca
     4801 ccaggctatt gaggttgagg ggctgcaggt cgagtttgat ctgcccttct tcgagtttgg
     4861 cgtattccag caagcggttg ataagggcct ccatggcgct taagtcctgt tcgatgcgct
     4921 gtaaataacg ggttgattgc tcggcagttt gggcctcttc gagcatggca aaaccgaacc
     4981 gcaagcgggc catgggggtt tttaagtcgt gggagacggc ccggctgagc aggcggttat
     5041 cagccaaaag gctttgcacc cgttcggcca tctgattaaa ggcttcctcc agtgagcgga
     5101 tataggacca gcggctgggc gctaagcggg cgttaagatc gccagcgcca aaggcgcggg
     5161 cggattcctg caataacatc aagcgccgca ccagggggta gaggcacaac accaggataa
     5221 gaatgagccc gcaataaaat accaaggtga gggtgatgct ggcctgctcg ttggagcttg
     5281 cggggcttgc cactgccagg gtgagcaccg ccttctgctt gggtaaaaaa taattaatca
     5341 gcaaatggcg gttgtcttcc agtagcaccg gctcgccgct ttccagcttg gcgtgcag
//
LOCUS       DAOQGS010000267         5361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7822_length_5361_cov_4.362797, whole genome shotgun sequence.
ACCESSION   DAOQGS010000267 DAOQGS010000000
VERSION     DAOQGS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7822_length_5361_cov_4.362797"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>5361
                     /locus_tag="PKZ52_10690"
     CDS             246..>5361
                     /locus_tag="PKZ52_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNG60282.1"
                     /translation="MKRNSKWQTLGKTATGLAMIPLLATLLATAGCGKSKSTATPVVS
                     GSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGIALKGAAVD
                     GPMANAVVRVYRVDTTKANLEGQLLDEGVTDAAALFSGVVIPAGEDGPFLIVITSTDA
                     TLDLNTGKKPVIGEVRTIVTKDGAAKPVYATPLTTMAVGLAASVTSDGASSQELLTKL
                     GESATQVVSALGMGMSSDLNIFTTPPLVTDNTNTTGELKDVAAYRAAVAGAASIIHTI
                     QQNNENGADSRDVLLSLAQDLGDGKIDGKDKDNKALTVYDSNQAAVIVETDPGSLLIP
                     GTNVPVSSIQTLLASEAKDTGTKTNTDNLTNGQIVTEVIAAVVNTDKDGDGVLNSEDA
                     FPNDPTESKDTDKDGVGNNADTDDDGDGVEDSQDAFPLNPFETRDTDKDGKGDNEDSD
                     DDGDGVPDAQDAFPLDPTRSNKNDQDNDGWPNGQDPDDNNAKVPGVPFVDTDKDGVAD
                     LYDEDDDNDGVPDVDDALPLDKRSSKDTDGDGFGDNLDDDIDGDGVPNNTGGNDVPNS
                     KENRAGIADAFPFDPTESADLDKDGIGDNKDADKDGDDLPDVNDKDPSNPDQDKDGVR
                     DGKDAFPNDATETLDTDGDGIGNNKDNCKFVPNKNQTDTDGDKIGDACDADADGDGVP
                     NNDDPAPLDAKIKLREDNDGDGWLKGQDPDDNDANKPGIPFVDTDKDGLADDGGLQPN
                     PDDDNDGVPDKDDAFPKNKEEQFDFDQDGKGDRSDDDDDNDGVLDINDRFPRNGEESA
                     DADKDGVGDKLDNCPNVPGPQTDTDNDGKGNVCDEDDDNDGVKDFQDAFPLDPKESKD
                     TDGDKIGNNADLDDDGDGLSDLEEAEKGLNPAERDTDRDSISDKADNCPLVPNSDQTD
                     SDSDGKGDLCDNPPKLEGFYLLDTTVKEQSKTLKPGSELAEQKAAELCGNELNDLQSQ
                     VYYIQQKEAELRLTGQDRKFQVSQGIPARINSFGQFNFDQRQEHTLDQGLFMRSRLIF
                     NGSLASSGTVNGSAVEEVSLVRNSNGASETLLTCKTSFAVLFSPMAAADTAVTLDAKG
                     EDGGFSSTAGERRWNPETQSDSLNFGYFTINSGGDFAHFWNPKDQAWQADEGKGESGG
                     FRLTLAGWIQASGDGMINISDAGVTFEQADDLGNILEKWQVHAFKAPAAKHAMDQLVD
                     KLWVEEALQNPNALFSQGSVLAIEAVSLMDTYTVECGEMPLGNLACDNGLALEWLDGK
                     PVFADSFSDMIHPIDSVMSNPGQGIWMSHSPNGEIYAYIQGGGDATAGSVAFYVQRMN
                     GDTSSFTQLKNAAGVAVMSQWSIQQPYEANGVQLLMVNMPEYLQTTFGADDNDGMFLA
                     VINDSSDSKPYVRIGHFQKAGRVHRESGLNQAALKEALANFDFDLPPDADGDGVSDDR
                     DAFPNDPSEQFDSDKDGVGDKKDAFPFNPNEQKDTDHDGKGDNADDDDDGDTIPDATD
                     PNPTTPDAHVPGPGPVPVGDAAKGREAYGSTCASCHGSDGKGTMVAPALFPLKNKYLV
                     KDAAGEHELVLEAYIGPHVNKPEQTPACNQDCALNITAYLRSLQGNGTVDGDYDHDGL
                     SDSVDPDDDSDQIPDAEDPEPYSPLDMDKDGVPDNQDAFPFDPKETRDTDHDGKGDNA
                     DDDDDGDGIPDSEDLDDDGDGIPDSEDPDSHTPPPQV"
BASE COUNT         1422 a         1559 c         1447 g          933 t
ORIGIN      
        1 aaattcctgt tgttaatagt cagaatgccg ccatttttta ttcgcttttt ttaccgatgc
       61 aattttgttg gaaacaaaca atataaaaaa ccgcctttat tttttgcagg tgtttcgcta
      121 ccgctgtggg cgctgcaggt gctgttaatt caataaaaaa agcgtggcta tactccactg
      181 gatgttaggc gctggcgatt cgttcgcggt gctgtaatga ggtaacaaca acggtggaag
      241 aattcatgaa aagaaattct aaatggcaaa cacttggcaa aacagcaacc ggcttggcga
      301 tgatcccgct gctggcaact ctcttggcaa cggcgggctg cggtaaatcc aaatcaacgg
      361 caacgccggt ggtttcgggc agcagctcca gttcatccag tagttccagc tcttcgagca
      421 gctcaagttc ttcgagcagc tcaagctcct caagcagttc tagttcatcc agcagctcaa
      481 gctcgtcgag cagctccagc tcatccagtt cgtccagttc cggtatcgcc ctaaaaggcg
      541 ctgcggtgga tggccctatg gccaacgcgg tggtgagggt gtatcgcgtt gataccacta
      601 aggcgaattt agaaggccaa ttgttggatg aaggtgttac cgatgccgct gcactgttca
      661 gtggtgtggt gattcccgcc ggtgaagacg ggccgttttt aattgtgatt acctccaccg
      721 acgccacctt ggatttgaac accggcaaaa aaccggtgat tggtgaggtg cgcaccatag
      781 taaccaaaga cggtgcggct aaaccggttt acgccacccc tttaaccacc atggcggtgg
      841 gacttgcggc cagcgttacc agcgatggcg ccagcagcca agaattactc accaagttgg
      901 gcgagtctgc cacccaggtt gtatccgcct tgggtatggg catgtcctct gacttaaata
      961 ttttcaccac gccgcctttg gtcactgata acaccaacac caccggcgag ttaaaagatg
     1021 tagccgccta ccgtgccgcg gtggccgggg cggccagtat catccacacc attcagcaga
     1081 ataacgagaa cggcgccgac agccgggatg tgctgctgag cctggcccag gacttaggcg
     1141 acggcaaaat tgatggcaag gacaaagaca acaaggccct taccgtctac gactccaacc
     1201 aggcggcggt gattgtggaa accgacccag gcagcctgtt aatccccggc accaatgtac
     1261 cggtcagcag cattcaaacc ctgttggcct cagaagccaa agacaccggc accaaaacca
     1321 acacggacaa cctcaccaac ggccagatag tcaccgaggt gattgcggcg gtggtgaaca
     1381 cggataaaga cggcgacggc gtgcttaaca gcgaggacgc cttccccaat gacccaaccg
     1441 aaagcaaaga caccgacaaa gacggcgtgg gcaacaatgc cgataccgat gacgacggcg
     1501 atggagtgga ggatagccag gatgccttcc ccctcaatcc attcgaaacc cgcgacaccg
     1561 acaaagacgg caagggcgat aacgaagaca gcgacgatga cggcgacggt gtacccgacg
     1621 cccaagacgc cttcccccta gaccccaccc gcagcaataa aaacgaccaa gacaacgatg
     1681 gttggcccaa cggccaggac ccagacgaca acaatgccaa ggtgcccggt gtaccttttg
     1741 tggatacgga taaagacggc gtggccgacc tctacgacga ggacgatgac aacgacggcg
     1801 tacccgatgt ggacgacgcc ttacccctgg ataaacgctc ctccaaagac accgatggcg
     1861 acggctttgg cgataacctg gatgacgaca tagacggcga cggcgtgccc aacaataccg
     1921 gcggcaacga cgtgccgaac agtaaagaga accgcgccgg tatagccgat gccttcccct
     1981 ttgaccccac cgaaagtgct gacctggata aagacggcat aggcgataac aaggatgccg
     2041 acaaagatgg cgacgacttg ccggatgtga atgacaaaga cccgtccaac ccagaccaag
     2101 ataaagacgg tgtgcgcgat ggcaaagacg ccttccccaa cgacgccaca gagaccctgg
     2161 ataccgacgg cgacggcata ggtaacaaca aagacaactg taagtttgtg cccaacaaaa
     2221 accaaaccga taccgatggc gacaagatag gcgatgcctg tgatgccgat gcggacggcg
     2281 atggcgtacc caacaacgac gaccctgcgc ccttagatgc caagatcaaa ctcagggaag
     2341 ataacgacgg cgacggctgg ttaaaaggcc aagacccaga tgataacgat gccaacaaac
     2401 ccggcatccc ctttgtggac accgacaaag acggcctggc cgatgatggc ggcctgcaac
     2461 ccaacccaga cgacgacaac gacggcgtgc cggataaaga tgacgccttc ccgaaaaaca
     2521 aagaagaaca attcgacttt gatcaagacg gcaagggcga ccgcagcgat gacgacgacg
     2581 ataacgacgg cgtcctcgat attaatgacc gcttcccccg caatggcgaa gaatctgccg
     2641 atgcggataa agacggcgtg ggtgataagc tcgacaactg ccccaatgta cctggcccgc
     2701 aaactgatac agacaatgac ggcaagggca atgtgtgcga tgaggacgat gacaacgacg
     2761 gtgtgaagga cttccaagac gccttcccgc tagatcctaa agagtccaaa gacaccgacg
     2821 gcgacaaaat tggcaacaac gccgacctgg acgatgatgg cgacggcctg tccgatttag
     2881 aagaggctga gaaagggctt aaccctgctg agcgcgatac cgaccgggac tccatcagcg
     2941 ataaagccga caactgccct ctggtgccca actccgatca aaccgacagc gacagcgacg
     3001 gcaagggcga cctgtgcgac aacccgccca agctggaagg cttttatctg ctcgatacca
     3061 ccgttaaaga gcagagcaaa acccttaaac ccggctcaga actcgccgag caaaaagccg
     3121 ctgaactctg cggcaacgag cttaacgacc tgcaatccca ggtgtactac atccagcaaa
     3181 aagaggcgga attgcgctta accggccagg atcgcaagtt ccaagtcagc caaggcattc
     3241 ccgcccgcat caactccttt ggccaattta acttcgacca acgccaagaa cacaccctgg
     3301 atcaaggcct atttatgcgc tcgcgcttga tctttaacgg cagcctggcc agcagcggca
     3361 cagtcaatgg ctccgccgtg gaagaggtga gcctggtgcg caacagcaat ggcgccagtg
     3421 agacactgtt aacctgtaag acgagttttg cggtgttgtt tagccctatg gcggcggcgg
     3481 atacggctgt gaccctagat gccaaaggtg aagacggcgg ctttagcagc accgctggcg
     3541 agcgccgctg gaaccccgaa acccagagcg acagcctgaa ctttggttat ttcaccatta
     3601 atagcggcgg cgattttgcc cacttctgga accctaaaga ccaagcctgg caggctgacg
     3661 aaggcaaggg cgagtcggga ggcttccgct taaccctcgc tggctggata caagccagtg
     3721 gcgacggcat gattaatatc agcgatgccg gtgttacctt cgagcaggcg gatgacctgg
     3781 gcaacatatt ggaaaaatgg caagtgcacg ctttcaaagc ccccgctgcc aagcacgcta
     3841 tggatcaatt ggtggataaa ctctgggtgg aagaagccct gcaaaacccc aatgcgctat
     3901 ttagccaggg ctccgtgttg gccatcgagg cggtaagcct tatggatacc tacacagtgg
     3961 agtgtggtga gatgcccctg gggaatttgg cctgtgataa cggcctggcc ctggagtggc
     4021 ttgatggcaa gccggtgttc gccgatagct tcagcgatat gatccacccc atcgacagcg
     4081 tcatgagcaa cccaggccag ggtatctgga tgagccatag ccccaatggc gaaatctacg
     4141 cttatataca aggtggtggc gatgccaccg ctggttccgt ggccttctac gtacagcgca
     4201 tgaatggcga caccagcagc tttacgcagt tgaaaaacgc cgccggtgtg gcggtaatga
     4261 gccagtggag catccagcag ccctatgaag ccaatggcgt acagctgtta atggtgaaca
     4321 tgccggaata tctgcaaacc accttcggcg ctgacgacaa cgacggcatg ttcctggcgg
     4381 tcatcaacga cagctccgac agcaaaccct atgtgcgtat cggccatttc caaaaagctg
     4441 gccgagttca tcgcgaatca ggccttaacc aagctgcttt aaaagaagcc ttggctaact
     4501 ttgactttga cctgccaccg gatgccgatg gtgacggtgt gtccgacgat cgtgacgcct
     4561 tccccaacga cccatctgag cagttcgata gcgacaaaga cggcgtaggc gataaaaaag
     4621 acgccttccc cttcaacccc aatgagcaaa aagacaccga ccacgatggc aaaggtgaca
     4681 atgcggatga cgatgacgat ggcgacacca tcccagatgc caccgaccct aaccccacca
     4741 cgcccgatgc ccacgtcccc ggccctggcc ccgtacctgt aggggatgcg gcaaaaggcc
     4801 gcgaggccta tggcagcacc tgcgcctcct gccacggcag cgatggcaag ggcaccatgg
     4861 tggcaccagc gctcttcccc ttaaagaata agtacctggt aaaagatgcc gctggtgagc
     4921 acgagctagt acttgaggcc tatatcggcc cccatgtaaa caagcccgaa caaaccccag
     4981 cctgtaacca ggattgcgcg ctcaacatca ccgcctacct gcgcagcctg cagggcaatg
     5041 gcacagtaga tggcgactat gatcacgatg gtctctccga cagcgttgat cccgacgacg
     5101 acagcgacca aatccccgac gccgaagacc ccgagcccta ctcgccgttg gatatggata
     5161 aagacggtgt gcccgacaac caagacgcct tcccgtttga ccccaaagaa acccgcgaca
     5221 ccgaccacga cggcaagggt gataatgcgg acgatgatga cgacggcgac ggtatacccg
     5281 acagcgaaga ccttgatgac gatggcgacg gcatccccga cagcgaggac ccagacagcc
     5341 acacaccgcc cccgcaagtg g
//
LOCUS       DAOQGS010000268         5360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7827_length_5360_cov_4.569651, whole genome shotgun sequence.
ACCESSION   DAOQGS010000268 DAOQGS010000000
VERSION     DAOQGS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_5360_cov_4.569651"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKZ52_10695"
     CDS             <1..400
                     /locus_tag="PKZ52_10695"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNG60283.1"
                     /translation="AAGLSPFAPEEAAISAGRLMLQEIANCVKRGENFAIETTLAGLN
                     YLRHIEKWKLAGYRVYLFFLSLQSAEYAVVRVQQRVKHGGHSIPEHVIRRRFAVGLDN
                     FNQYYRHAVDTWSLYDNTWTTPILLEWGEN"
     gene            400..597
                     /locus_tag="PKZ52_10700"
     CDS             400..597
                     /locus_tag="PKZ52_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60284.1"
                     /translation="MKGEDIGLAKDQLLKDAVIAMQRAALMARKNAIQTDTALVVRRG
                     DQMVLVSAEELRKQEGHFSDQ"
     gene            677..1549
                     /locus_tag="PKZ52_10705"
     CDS             677..1549
                     /locus_tag="PKZ52_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60285.1"
                     /translation="MARYVNAIHAELFGGLKKVNLQLDGKNLILVGGNACGKTQLINL
                     LFGELRSRVIERNNKSLREVEQSYSDAKQRLSSCSKASQEHAFWQSELQRHQKELGQL
                     KKLAVGLVYLEDFVIAYHAHKAVLVSFDAHRKAEIRESASSKSLAELAREAGSGAANT
                     FAAVFEGFLVSHKTQQAYAQALDDNLSKAENIRMWFDKLEADLRELFEDASLVLQFNS
                     ESQSFLINQNGKAPYSFQQLLSGFSSILAIYAELIARVELKRLIPRTYREWFLLMRSM
                     CICMSRCNGRYLHF"
     gene            1636..1905
                     /locus_tag="PKZ52_10710"
     CDS             1636..1905
                     /locus_tag="PKZ52_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60286.1"
                     /translation="MSRGEEVGDVSMYSYEALLEGLFGVLAVSDILKGKIIELATLAG
                     MPEPNFTRMRELVLEIDDAEDKLDPESLFYLNRAKISLGKAGFGV"
     gene            1907..2410
                     /locus_tag="PKZ52_10715"
     CDS             1907..2410
                     /locus_tag="PKZ52_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60287.1"
                     /translation="MFNVERTPCAPPSLLKQKKYDDNDVWQALENIFYKKCYLCETVQ
                     PLDLNVEHLKSHKGDKALKFSWGNLYFACARCNNIKSTRYDDILDCCNPGENVFESIK
                     CLPPHSPRSSRLVVEALSQYDRVIKTCELLNEIYNNASSVNKKNFGSLFAQQGMGALC
                     SAVKVYG"
     gene            3005..4495
                     /locus_tag="PKZ52_10720"
     CDS             3005..4495
                     /locus_tag="PKZ52_10720"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNG60288.1"
                     /translation="MSYKQLHHHFTKISHYNHLSAICSWDQSAMMPAGGNAARGEAMA
                     ELAVLIHQNTTAPQLAEWIKTAEQEQLTPQQQASLAAIKRRWQQASQVPEDLVQAQSL
                     ATSTCEHAWREQRKNNDWAGFKPNLEAVVKLAREEAGVRAAGTLTHRYDALLELYEPG
                     MTASRIDEVFGAVKTWLPEFITKALDIQSRKTIVAPQGPFAVPQQKELGMAVMQLLGF
                     DFNHGRLDVSTHPFCGGVPSDVRITTRYDESDFMKALLGVIHETGHARYEQNLPIDWR
                     NLPVGEARSMGVHESQSLFFEMQLARSAEFSQLIAPLAAKILGRQDDPAFNQQNLYAY
                     NTRIKRGFIRVDADELTYPAHVILRYEIERALIEGDIEVADIPDLWGEKMQAYLGLST
                     VGNFKDGCMQDIHWPLGSFGYFPSYTLGAMYAAQLFNAAKQVIPKLSDKISAGDLAPL
                     FAWLNEHIWAKASFMATDDLIAGATGEPLNAKYFKQHLEQRYLQTL"
     gene            4504..5163
                     /locus_tag="PKZ52_10725"
     CDS             4504..5163
                     /locus_tag="PKZ52_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60289.1"
                     /translation="MIQFYLSKDAAADFKHLVEAPLVENYQAMQWYLHRVTIARRKCI
                     IAMEKQSRYAITFCGLTKKDLADFPQLFAYWFSLHGLASVVEQHGEDDVLEQRVLELA
                     EVITSGQTYQQGYDRSVTAHISQVVDLLRYYVEERGYPLPVEPREAAIFSTRANELLR
                     KKSGSDEYFLPVTEFSDFWQGLLDLKIPGESGLRPSKDAEPKYTDNVIQVDFRASQRK
                     S"
BASE COUNT         1440 a         1053 c         1386 g         1481 t
ORIGIN      
        1 tgctgcaggt ctgtcgccat ttgcaccgga agaagcagca attagtgccg ggcgtttgat
       61 gctgcaggaa atagcaaact gcgtaaaacg cggcgagaat tttgccattg aaacaaccct
      121 agcaggttta aattatttgc gtcatatcga aaaatggaag cttgcaggct acagggttta
      181 tttgtttttt ctctcattgc aatcggcaga atatgcggtt gttcgtgtgc agcaacgtgt
      241 taaacatggt gggcattcaa tacctgagca tgtaattcgc aggcgctttg ctgtggggct
      301 agataatttt aatcaatact atcgccatgc cgtagatact tggtcactct acgacaatac
      361 ttggacaaca ccgatactgc ttgagtgggg agaaaattaa tgaaaggcga agatataggt
      421 ctagctaaag atcaattgtt aaaagatgca gtaattgcaa tgcagcgcgc tgcgcttatg
      481 gcaagaaaaa atgcaatcca gactgatacg gcattggttg ttagaagggg ggatcaaatg
      541 gtgttggttt cagctgaaga gttgcgcaag caagaagggc atttcagtga ccaataagca
      601 tattgcctga ttggcttttt aggtgtttca cgattagacc attaaactac catgaaagtt
      661 agttagggtg gaaaccatgg caagatatgt aaacgcaatt catgcagaac tttttggtgg
      721 tttaaaaaaa gtaaatttgc agcttgatgg aaaaaatctg atattggtag gaggaaatgc
      781 ctgcgggaaa acgcagttga taaatctcct gtttggcgaa cttagaagcc gagttatcga
      841 aagaaataat aaaagtttgc gggaagttga gcaaagttat tcggacgcaa agcagcggct
      901 ttcaagctgc agtaaggcaa gtcaggagca cgcattttgg caaagtgagt tgcaaaggca
      961 tcagaaagaa ttggggcaat tgaagaaact ggcggttgga ctagtatacc tagaggattt
     1021 tgtgattgca tatcatgcgc acaaagctgt attggtcagc tttgacgcgc accgaaaagc
     1081 agaaatccgc gaatccgcat cgtcaaagag cttggcagag ttggctaggg aggcagggtc
     1141 tggtgcagcc aatacatttg cggcagtatt cgagggcttt ttggttagcc ataaaacgca
     1201 acaagcgtat gcgcaagcat tggatgataa cctctcgaaa gctgaaaata tcagaatgtg
     1261 gtttgataag ctagaagccg acctgcggga attatttgag gatgcttcac tggtattgca
     1321 atttaactca gaatcacaat catttttaat taatcaaaat ggcaaagctc catatagctt
     1381 tcaacagttg ttgtcagggt tttcttcaat tttggcaata tatgccgaat tgatagcaag
     1441 ggtggagctt aaaagattaa tcccgaggac ttatcgggag tggtttttat tgatgagatc
     1501 gatgtgcatt tgcatgtctc gctgcaacgg aagatatttg catttttgac aaagtcgttt
     1561 cctggtgtgc agtttatagt ttcgacccat tccccatttg ttgtatcttc agttgataac
     1621 gcggtcatat atgatatgag ccgtggtgag gaggttggag atgtatcaat gtattcttac
     1681 gaagcattgc ttgaaggttt gtttggtgtt ttagctgtat cagatattct caagggcaaa
     1741 ataattgaat tggcgacgtt ggctgggatg ccggagccaa atttcacaag aatgcgtgag
     1801 ttggtgcttg aaattgatga tgctgaggat aagcttgatc cagagtcatt gttttatttg
     1861 aatcgagcta aaatctcgct tggaaaagca ggttttggag tttgaaatgt tcaatgttga
     1921 acgcactcca tgtgcaccgc cctctttgct gaagcagaag aaatacgacg acaatgatgt
     1981 ttggcaggcg ctggagaata ttttctataa aaaatgttat ttgtgtgaga ctgtgcagcc
     2041 gcttgattta aatgtggagc acctaaagtc gcacaagggt gacaaagcat tgaagttttc
     2101 ttggggtaat ctctattttg cgtgtgctcg ctgcaataat attaagtcga caagatatga
     2161 cgacatattg gattgctgta atcctggtga gaacgtcttc gaatcaatta agtgcttgcc
     2221 gccgcattcg ccacgatcta gccggttggt tgtagaggcg ttgtctcagt atgaccgagt
     2281 aataaaaacg tgtgaactgc taaatgaaat ttacaataat gcttctagtg tgaataaaaa
     2341 aaatttcggc agcttatttg cgcagcaagg tatgggagcg ctttgctctg ctgtcaaagt
     2401 atatggttag ttattttgct gtagacgcta cggactcaga aaagcagatt gcacttgaaa
     2461 agataggtat tttgcttcgg agggatcaag cctattccgc cttttctagg tgggtcgtgt
     2521 tatgtgatga ggagttgagt aatctattgg gtcatttgtt ggattgatgg ctgaggtaat
     2581 tcatgtattc ccttaatgtt ttcattggtt gtaaaggtga ggctgtatta atttgtagtg
     2641 agtgatattc attaacaatg gagtgcgcca gtgttcgcta ttcattattt ccaatttttc
     2701 ccaattggtc tcaatatatt tcaaaatatt tttaattttt gctaataaaa agcggctaaa
     2761 tgcctatgtc aaagcctaaa aacctagccc tatcaatatt tctggtctta atctgcggat
     2821 gcgccgagaa cgccgcctac aacaccgccc agcgcagcgc tcaggtagag tgtaataaag
     2881 aacccacgca agaattgtat caacagtgta taaacaaaaa ttccatgccc taccgagagt
     2941 ataaggccga gaaaaatcgg ccttaaattt catctaaaga attcgcacgc cattgggggc
     3001 tgctatgagt tacaaacaac tacaccacca cttcaccaaa atttcccact acaaccacct
     3061 aagcgccatt tgcagttggg atcagtccgc tatgatgcca gcaggtggca acgcggcgcg
     3121 gggggaggct atggcggagt tggcggtgtt gattcatcaa aatactacag cgccgcaatt
     3181 ggcagagtgg attaaaaccg ccgagcagga acaattaacg ccgcaacaac aggcgagcct
     3241 ggcggcaatt aaacgccgct ggcagcaggc cagccaagtg ccggaggatt tggtgcaggc
     3301 gcagtcttta gcgacttcaa cctgcgagca cgcctggcgg gagcagcgca aaaataatga
     3361 ctgggcgggc tttaaaccga atttagaagc agtggtaaaa ctagcccggg aggaagcggg
     3421 tgtgcgggcg gcgggcactt taacccaccg ttatgatgcg ctgttggaat tgtacgagcc
     3481 gggtatgacg gcatcgcgta ttgatgaagt attcggcgcg gtaaaaacct ggttgccgga
     3541 atttattact aaggcgttgg atattcaaag ccgtaaaacc attgtggcgc cccaagggcc
     3601 atttgccgtg ccccagcaaa aagagttggg tatggccgtt atgcaattgt tggggtttga
     3661 ttttaaccac ggccgcttgg atgtgagcac ccatccattt tgcggcggtg tgcctagtga
     3721 tgtgcgcatc accacccggt atgatgaaag cgattttatg aaggccttgt tgggggtaat
     3781 ccacgaaacc ggccacgccc gctacgagca aaatttgcct attgattggc gcaatttacc
     3841 cgtaggtgag gcgcgctcta tgggtgtgca tgaatcccag agtttatttt ttgaaatgca
     3901 actggcgcga tcggcggaat ttagccaatt aattgcgccc ctggcggcga aaattttagg
     3961 ccgccaagat gacccagcgt ttaaccagca aaacctctac gcttacaaca cacgcattaa
     4021 gcgcggcttt attcgcgtgg atgccgatga attaacctat ccggcccatg taattttgcg
     4081 ctatgaaatt gagcgcgcct taattgaggg cgatattgag gtggcggata tccctgatct
     4141 atggggtgaa aaaatgcagg cttatttagg tttatctaca gtgggtaatt ttaaagacgg
     4201 ctgtatgcag gatattcact ggcccttggg ttcatttggt tatttccctt cttatacctt
     4261 gggcgctatg tacgcagcgc aattgtttaa tgcggcaaaa caggttattc ctaaactcag
     4321 tgataaaatc agcgccggtg atttggcacc cttattcgct tggttaaacg agcatatttg
     4381 ggccaaggca agttttatgg ccaccgatga tttaattgct ggcgccaccg gcgagccgtt
     4441 aaatgccaag tattttaaac agcatttaga acagcgttat ttgcaaacgc tttagagggc
     4501 gccatgatcc agttttattt aagcaaagat gccgctgcgg attttaaaca cctggtggaa
     4561 gcgccgctgg tggagaatta tcaagccatg cagtggtact tacaccgggt taccatcgcc
     4621 cggcgcaaat gtattatcgc tatggaaaaa caaagccgtt atgccattac cttttgcggt
     4681 ttaactaaaa aagacttggc ggacttcccg cagttatttg cctattggtt ctccttgcac
     4741 ggccttgcct cggtggtgga gcaacacggt gaggatgatg tgcttgaaca gcgggtgttg
     4801 gagctggctg aggtgattac ctctgggcaa acctaccagc agggttatga tcgctccgtg
     4861 acggcccata tttcccaggt ggtggatttg ttgcgttatt acgtggagga gcgcggttat
     4921 ccactgccgg tggaaccccg tgaggcggcg atattttcca cccgcgccaa tgaattgctg
     4981 cgcaaaaaat ccggctcgga tgagtatttt ttacctgtga cggaatttag tgatttttgg
     5041 cagggtttat tagaccttaa aataccgggc gagagtgggc ttagaccttc taaagacgct
     5101 gagcctaaat acactgataa tgtgattcag gtggattttc gtgcgtcgca gcgtaagagc
     5161 tgaggttggc tgtttcgcgg aaaattcttt aatttagata gggtaagtat gtgttagttt
     5221 ttcgcttgtt cttaaattgt gccgggccgg gttttctaac cccaacccaa tgtctttaag
     5281 tcgagcgctt taaggcgaat tttacccgta ggtcaatgag atggactccc cctctctaac
     5341 aacccggcat cgaagtgcca
//
LOCUS       DAOQGS010000269         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7850_length_5346_cov_4.189000, whole genome shotgun sequence.
ACCESSION   DAOQGS010000269 DAOQGS010000000
VERSION     DAOQGS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7850_length_5346_cov_4.189000"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKZ52_10730"
     CDS             complement(<1..929)
                     /locus_tag="PKZ52_10730"
                     /EC_number="2.7.7.42"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="catalyzes the ATP-dependent addition of AMP to a
                     subunit of glutamine synthetase; also catalyzes the
                     reverse reaction - deadenylation;
                     adenylation/deadenylation of glutamine synthetase subunits
                     is important for the regulation of this enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HNG60290.1"
                     /translation="MNAIPLPAEFLDQFTQAFNSTGLSTPLTDEQTQHLKRVLIGSHY
                     ARQQFQRNPNWLEQLLANPMSEAPPLLLEGDEITAGKALRLYRHQQMLGIIWRDLNRL
                     VSPQATCAALSQLADTCIQAALEYLYPLLTASFGEPVDKLGKRQGLFVIGMGKLGSRE
                     LNLSSDIDLIFAYPTSGHTQGIKSISNSEFFAMLGQRLIKLLDANTAEGFVFRVDMRL
                     RPFGQSGVLACSFEALEDYYHNHGREWERFAMIKARLSAHNGDAFEAEQLMSILRQFT
                     YRKYTDFSVIQALRSLKLMISREVNKKGKEEDV"
     gene            complement(950..1882)
                     /locus_tag="PKZ52_10735"
     CDS             complement(950..1882)
                     /locus_tag="PKZ52_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNG60291.1"
                     /translation="MLLLAIRARQMSLMVIGLLLCSASFAFTGNIALTFDDAPTADSP
                     LFSGESRTTKIIATLIAQKVPDALFFVKADTLPPATINRIKRYTDAGLHIANHSFSHV
                     SANNVPADEFLVDAYRAHLLLKGLDNFLPYFRPPYLHYGKDMEAIAYIQKNLAALNYK
                     DGFVTIDNADWAINALLVKAASEGKKIDLKKARRLYVQVLIQAIEFFDALAQKTYGHS
                     PNHVLLLHENDTTALFLGDLIAQIRKSGGHIISPQEAYADPAYQQFPMSYFQKQGRVA
                     AAAQLQGVPIEQLKHPAEDSQYLEALFNQHAVFK"
     gene            complement(1921..2373)
                     /locus_tag="PKZ52_10740"
     CDS             complement(1921..2373)
                     /locus_tag="PKZ52_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60292.1"
                     /translation="MSDIHIDDFYRDTARILVALYNQFPRKTTLYVEDIAGPDKPDEF
                     GLHSPRHLACFHTILWLAQTDYVHYESLIRQEAADQIVLSHRGFLLLTSMHNSEKKDI
                     EANIPATPGISLQEHLLVNRIRRELKEGSSFSLAALMRNTLLHSRSFP"
     gene            complement(2481..3704)
                     /locus_tag="PKZ52_10745"
     CDS             complement(2481..3704)
                     /locus_tag="PKZ52_10745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNG60293.1"
                     /translation="MQRVLKSKKLDGVCYDIRGPVLDHANRLEEEGHRILKLNIGNPA
                     PFGFDAPDEIIADVIHNMRAAQGYTHSKGLFQARKAIMQECQRLQVPNVEIDDIFLGN
                     GVSELIVMANQALLNPGDEVLIPSPDYPLWTAAVNLAGGKAVHYLCDESADWFPDLAD
                     IRAKINSRTRAIVVINPNNPTGAVYSQELLEQIVQIAREHRLVIFSDEIYSKILYDDA
                     QFIPMGRLATDVLCLSFNGLSKTYRLAGFRSGWVVISGAKQLATEYIKGIEMLASMRL
                     CANVPAMFAVQTALGGYQSINELILPGGRLLEQRDAAYNSLVAIPGISCVKPRGSIYL
                     FPKIDRSVYKYSDDQQLVLDFLIQQKVLLVQGTAFNWPEPDHLRIVFLPVKDEINKAI
                     GKLGEFLATYTPLDL"
     gene            3771..4172
                     /gene="msrB"
                     /locus_tag="PKZ52_10750"
     CDS             3771..4172
                     /gene="msrB"
                     /locus_tag="PKZ52_10750"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNG60294.1"
                     /translation="MSDSQLKNDEYWRANLSEDEFRICRQKGTERAFSGRYWNHKALG
                     IYTCRCCGAPLFNAEAKYDSGSGWPSFFTAINKEAVSEAPDTSHGMRRVEILCERCGS
                     HLGHVFPDGPEPTGLRYCVNSASLGFVAQED"
     gene            complement(4169..4948)
                     /locus_tag="PKZ52_10755"
     CDS             complement(4169..4948)
                     /locus_tag="PKZ52_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60295.1"
                     /translation="MSSVYVISSIVILLAILACYAFVSQTLALRRQHRERLLTVLTAK
                     VRNFKHMLSGFPVGFLPKDLTVLVLRSLIDMYEQLVDIDPDNNQYKQDLQSVSQQLNE
                     VQRQTKPVAPAVIDNPQQLKDAKTCLEELHKFVFQLEGKGALNRTQADNYRNLIKQLV
                     LQVTVDAYLFSGRHAKQANKTRLAIHYFGLAHKLLSKENKGGTQDKKIAAITQILQEL
                     QEKLHEEEPHATVAAKPAETTEADWNKIKPDDSWKKKQVYD"
     gene            5214..5289
                     /locus_tag="PKZ52_10760"
     tRNA            5214..5289
                     /locus_tag="PKZ52_10760"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5248..5250,aa:Glu,seq:ttc)
BASE COUNT         1262 a         1261 c         1427 g         1396 t
ORIGIN      
        1 tttacatcct cctctttgcc ttttttattc acctcccggc tgatcattaa tttaaggctg
       61 cgcagggctt gaatgaccga aaaatccgta tatttgcggt aggtaaattg gcgcagaata
      121 ctcattaatt gttcggcttc aaaagcgtca ccattgtggg cgcttaggcg cgctttaatc
      181 atggcaaagc gctcccactc ccggccgtgg ttgtggtaat aatcctccag cgcttcaaaa
      241 ctgcaggcca gcacaccgct ttggccaaac ggccgcaggc gcatatccac acggaataca
      301 aaaccctcgg cggtattggc atccaataat ttaattagcc gctggccgag catggcaaaa
      361 aattcactat tgctgatgct tttgattcct tgagtgtgcc cgctagtggg gtaggcaaaa
      421 atcaggtcta tatccgagga taaattcagc tccctagagc cgagtttgcc catgccaatc
      481 acgaacaaac cctgccgttt acccagttta tcgactggct cgccaaagct tgcagtcagc
      541 agtgggtaaa gatattccaa cgccgcttga atacaggtat ccgctaattg gctaagcgct
      601 gcgcaggtgg cctgggggct taccaggcga tttaaatccc gccaaattat gcccagcatt
      661 tgttggtggc ggtacagccg cagggctttg cccgccgtaa tttcatcgcc ctctaacaac
      721 aaaggcggtg cttcgctcat ggggtttgct aaaagctgct caagccagtt ggggttgcgt
      781 tggaattgtt ggcgggcgta gtggctgccg ataagtacgc gttttagatg ctgggtttgc
      841 tcatcagtta aaggtgttga aagacctgtg gaattaaacg cttgggtaaa ttggtctaaa
      901 aattctgctg gtaggggaat agcattcata tgaagtaccc tgggtgctgt tatttaaata
      961 cggcgtgctg gttaaagagc gcctctaaat attggctatc ttcagcgggg tgttttaatt
     1021 gttcaatcgg cactccttgc agttgggccg cagccgccac gcggccttgt ttttgaaaat
     1081 aactcatggg gaattgttgg taggcgggat cggcataggc ctcttggggg ctaataatgt
     1141 gcccgccgct cttgcgaatc tgggcaatta aatcacctaa aaataatgca gtggtgtcat
     1201 tttcatgcag caataacacg tggttaggag agtggccata ggtcttctgt gcgagcgcat
     1261 cgaagaattc tatggcttgt attaaaacct gcacgtagag gcggcgcgcc ttttttaagt
     1321 cgattttttt cccttcgctg gcggccttaa ctaaaagtgc attgatggcc cagtcggcat
     1381 tatcgattgt aacaaaacca tctttataat taagggcggc gaggtttttt tggatatagg
     1441 caatggcctc catatccttg ccatagtgca gataaggcgg gcggaaatag ggtaaaaaat
     1501 tgtccaaacc tttaagtaaa agatgtgccc ggtaggcgtc cactaaaaac tcatcggcgg
     1561 gtacattgtt ggctgataca tggctaaaac tgtggttggc gatatgtagc cccgcatcgg
     1621 tatagcgttt gatacggtta attgtggcgg ggggaagggt atccgcttta acaaaaaata
     1681 gtgcgtcggg gactttttga gcgatgaggg ttgcaatgat tttagtggtg cgactctcgc
     1741 cgctgaaaag tgggctgtcc gccgtggggg catcatcaaa tgtaagggca atattgcctg
     1801 tgaaggcaaa actcgctgaa cacagcagca agccaatcac cattaaactc atttgccggg
     1861 cgcgaatggc taaaagaagc ataggctgta ctcttaatag gattcttaaa tccgccacac
     1921 tcaggggaag gatcgcgagt gcaaaagtgt gttgcgcatt agggctgcta aggagaacga
     1981 cgagccttct tttaattccc gtcgaatgcg gtttaccaat aaatgctctt gtaggctaat
     2041 acctggggtt gctggtatgt ttgcctcaat atcttttttc tctgagttgt gcatgctggt
     2101 aagcagtaaa aacccgcgat ggctaagcac aatttgatcg gccgcctcct ggcggataag
     2161 gctttcataa tgtacataat ccgtttgcgc caaccataaa atggtgtgaa aacaggctaa
     2221 atgacggggg ctgtgcaggc cgaattcgtc cggtttatcg ggcccggcta tgtcttccac
     2281 ataaagggtg gttttgcgcg ggaattggtt gtagagcgcc actaaaatgc gggcggtatc
     2341 ccggtaaaag tcgtctatat gaatatcgct cacaaacatc tccccatcaa ttcgcaggtg
     2401 tttattttag gctgtacttt ttcgtgcgcc acaaacaaaa actgcgccga agcgcagctg
     2461 gtgatggtgc aggcaggact ttataaatct aagggcgtat aggtggccaa aaactcacct
     2521 agtttaccta tggctttatt gatttcatct tttaccggca aaaatacaat gcgcaagtgg
     2581 tcaggttccg gccaattaaa cgccgtgccc tgcaccaaca acactttttg ctgaattaaa
     2641 aaatccagca ccaattgttg atcgtctgaa tatttataaa ccgaacgatc aattttcggg
     2701 aataaataaa ttgagcccct gggtttaacg cagctgatgc cgggaatggc aaccagtgag
     2761 ttgtaggcgg catcgcgctg ctctaacaaa cgcccgccgg gcagaattaa ctcattaatg
     2821 ctttggtagc cgcccagggc ggtttgcacg gcaaacatag ctggtacatt agcgcacagg
     2881 cgcatagagg cgagcatttc tatgcctttt atatattcag tggccagttg tttagcgccg
     2941 ctaatcacca cccagccaga gcgaaaaccc gccaagcgat aggttttaga taaaccgtta
     3001 aagcttaaac acaatacatc ggtagccagg cggcccatgg gaataaattg cgcgtcgtcg
     3061 tagagaattt tgctgtaaat ctcgtcggag aaaatcacca agcggtgttc ccgggcgatc
     3121 tgcacaatct gttcgagcaa ttcttggctg tacaccgcac cggtggggtt attggggtta
     3181 atgaccacaa tcgcgcgggt gcggctgtta attttggcgc gaatgtcggc taaatcggga
     3241 aaccaatccg cgctttcatc gcataaataa tgcacggcct tgccaccggc taaatttacc
     3301 gccgccgtcc aaagtgggta atcgggggag gggattaata cttcatcgcc ggggtttaac
     3361 agtgcttggt tggccatcac aattaattcg ctgacgccat tgcccaggaa aatatcgtcg
     3421 atttccacat tgggaacctg cagccgctgg cactcttgca taatggcttt gcgggcttgg
     3481 aacaggcctt tgctgtgggt atagccttgg gcggcgcgca tgttgtgaat cacatcggcg
     3541 atgatctcat ccggcgcatc aaagccgaag ggcgccgggt tgccgatgtt aagttttaaa
     3601 atgcggtggc cttcttcttc taggcggttg gcgtggtcca gcacggggcc gcgtatgtcg
     3661 taacacacgc cgtcgagttt ttttgatttg agtacacgtt gcatcgcaag ccttttaacg
     3721 atttaatcca atgattcaat ttgtctttaa cagtagggcg gggccggatt atgtcagata
     3781 gccaactaaa aaacgatgaa tactggcggg cgaatctcag tgaggatgag tttcgcatct
     3841 gtcgccaaaa gggtaccgag cgagcattta gcggccgtta ttggaaccac aaggcgctgg
     3901 gcatctacac ctgccgctgc tgcggtgcgc cattatttaa tgccgaggcc aagtacgatt
     3961 cgggctccgg ctggccgagt tttttcaccg ccattaataa agaagcagta agcgaagcgc
     4021 ccgacaccag ccacggtatg cgccgggtag aaattctctg cgagcgctgc ggcagccatc
     4081 tgggccatgt gttccccgac gggcctgagc ccactgggtt gcgctactgt gtgaattccg
     4141 catccctggg gtttgtcgcc caagaggact aatcgtagac ttgttttttt ttccagctgt
     4201 cgtcgggctt tatcttgttc cagtccgcct cggtggtttc ggcgggcttg gccgcgactg
     4261 tggcgtgggg ctcttcctcg tgcaattttt cctgtaattc ctgcagtatt tgggtgatgg
     4321 cggcaatttt tttatcctgc gtgccaccct tattttcttt gctcaataac ttgtgggcca
     4381 ggccaaaata atggatggcc aggcgtgttt tgttcgcttg tttggcgtgg cggccggaaa
     4441 acaaataggc atctaccgtg acttgtaaca ccagctgttt aattaaattg cggtaattat
     4501 ccgcttgagt gcggttgagg gcgcctttgc cctccaattg gaagacaaat ttatgcaatt
     4561 cctcaaggca ggttttggcg tctttgagtt gttgcggatt gtcgataacc gcaggcgcca
     4621 ccggtttggt ctgccgctgc acttcgttga gttgctggga tacggactgc aagtcttgtt
     4681 tgtactggtt attgtccggg tctatgtcca ccagttgttc atacatatca atcaaactgc
     4741 gcagcaccaa caccgttagg tcctttggta aaaagcccac cgggaagccg ctgagcatgt
     4801 gtttgaagtt gcgcaccttg gctgttagta ccgttaagag ccgctcgcga tgctgcctgc
     4861 gcaaggccag cgtttgcgag acaaacgcat agcaggccag aatggccagc aggattacga
     4921 ttgagctaat tacgtagacg gaagacatag catccaggca gtaagggatt accctaagcc
     4981 tagtcgcagg ggggggcagc tgcaacttga ggcctgttct attcagtgac ggctgggctg
     5041 aggtaatttc cagcaaactg ccacagaatt cgccaggttg gccagttgct ggcctaagcc
     5101 ttagattgta ttgaattttt taaaataagt ggttgacagc ccccaggtgc atccctagaa
     5161 tgcgcaccac ttctggcggc aaccacccat taccaacgct gatcagaata gacgtcccca
     5221 tcgtctagag gcctaggaca ccgccctttc acggcggtga caggggttcg aatccccttg
     5281 gggacgccac ttgcgggaat agctcagtgg tagagcacaa ccttgccaag gttggggtcg
     5341 cgagtt
//
LOCUS       DAOQGS010000270         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7906_length_5322_cov_4.613442, whole genome shotgun sequence.
ACCESSION   DAOQGS010000270 DAOQGS010000000
VERSION     DAOQGS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7906_length_5322_cov_4.613442"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1031
                     /locus_tag="PKZ52_10765"
     CDS             141..1031
                     /locus_tag="PKZ52_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNG60296.1"
                     /translation="MPAIHAMTVDVEDYFHVAAFNKVISPEDWPNWPSRVEANTQRML
                     ELFAKHNIHITFFILGWVAERYPNLVRQIASAGHEIASHGYSHQLIYRQTPEVFRAET
                     LKSKQILEDISGQAVTGYRAASYSITRQSLWALDILGQLGFTWDSSIFPTRHDNYGIP
                     GSPEEPYVIITDSGARLTEFPLTTAKVLGQPIPAAGGGYFRQYPYWLSRWLFARASDQ
                     GRKSQIFYLHPWEIDPEQPRVANASWFSNFRHYTNLHRCLPRLERMLEDFAFGTMSQA
                     LQAAPLSQTLKQSDLHSCGK"
     gene            1151..1978
                     /gene="cysE"
                     /locus_tag="PKZ52_10770"
     CDS             1151..1978
                     /gene="cysE"
                     /locus_tag="PKZ52_10770"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNG60297.1"
                     /translation="MHPGHDPIWSAILAETRQEAQREPALASFLHATILNHATFEAAL
                     SFHLANKLGSTAISSLLVRELIEQALHQDASIGNAARADIQAVFERDSACEAYSLPFL
                     YFKGFHALQAYRVAHWLWKQGRKSLAYYFQSHISLCFGVDIHPAARLGKGLMLDHGTG
                     IVIGETSVVDDDVSIMQAVTLGGTGKESGDRHPKVGKGVLISAGAKILGNITVGEGAK
                     IGAGSVVLKPVAAHTTVAGVPAVVVGAPTTQSPAKDMIHEISCDELCAQLKQQAAKN"
     gene            complement(2004..2657)
                     /locus_tag="PKZ52_10775"
     CDS             complement(2004..2657)
                     /locus_tag="PKZ52_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60298.1"
                     /translation="MRYLLAALGLSISCFSGVLSAAPADTSKEERAAYYRYVNDQGVK
                     VMDHSIPPAFSQRGYEVLNAAGRVIKVVPPALTEQDIKALEARQAIKEEYEQLSRRYS
                     STQDIEDAKLRQLDRLDANIALVRGNINNLKRQIDGIGVKAAEFERAAKPVPPSLLDS
                     LAKTKTELANTENLMVIRMAEKQQVVDKFEREKSIFTQGKELVKASATPAPTAQKTN"
     gene            complement(2654..3301)
                     /gene="pyrE"
                     /locus_tag="PKZ52_10780"
     CDS             complement(2654..3301)
                     /gene="pyrE"
                     /locus_tag="PKZ52_10780"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNG60299.1"
                     /translation="MLPYQSQFIQLALACQALKFGTFTLKSGRQSPYFFNAGQFQIGS
                     ALAQLGRFYAQALVDANISFDMIFGPAYKGIPLATTTAVALADQHGRDVPYCFNRKEA
                     KTHGEGGQLVGAPLAGRVVIVDDVITAGTAVREVLEIIQAAGAEPAAIVIGLNRQERG
                     RGELSAIGELVQLTGVPVVSIIKLDHILSYLAADPAQAELVANIELYRKTYGVDA"
     gene            3681..4457
                     /locus_tag="PKZ52_10785"
     CDS             3681..4457
                     /locus_tag="PKZ52_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNG60300.1"
                     /translation="MRIISLSVDGIHQAASRGLYDWIAKQDADIVCLQDLRALESELD
                     HAVFHPEGYYSYFFDSGIKHYNGVAIYTRHAPKALIYGLGFASGVDMEGRYLQVDFER
                     YSIGTLLAPCAQAENESLEVKLKFFEDMQALMHKITRKRRHFIFCGNWAMAHTRKDVQ
                     NWQEHISTPGFLPQEQQWLNQAFNQLGYADAFRQVNKDMDEFSWWPSGQIGKGCGWRT
                     DYHIISEALKRKVEYATIYKTQEFSSHLPVVIDYDIDDLV"
     gene            4484..5143
                     /locus_tag="PKZ52_10790"
     CDS             4484..5143
                     /locus_tag="PKZ52_10790"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNG60301.1"
                     /translation="MGSHYVLIDYENVQPDNLAQLTDPAFRVLVFVGDKQIKIAFELA
                     AAMQALGARAEYVKIEGNGLNALDFHIAFYIGQLATSDKHSQFHIISKDTGFDPLIRH
                     LKHKHIQIERREELTALLKPKPQTVKTPQPAAQAAAPAPPATNPVAKVFAYLRIDTNP
                     RPKTRTSLLNFLNSHLKQQFDKNALNTLVDELIDRKIIVADGDKISYPPPKPSQKAPQ
                     V"
BASE COUNT         1231 a         1566 c         1374 g         1151 t
ORIGIN      
        1 cgggactatt accaggctta aatttactgc ctaggcttta atgccgcaga aaataggccc
       61 acacgcgggc aaccggaacc ctcaacaggc ctttggcttg aagccaaaag cgtttacttc
      121 tcagctaaag atactgattt atgcccgcca ttcacgctat gacggtggac gtggaagact
      181 acttccatgt cgctgccttc aataaggtta tctcccctga ggactggccc aactggccct
      241 cgcgggtgga ggccaatacc cagcgcatgc tggagttgtt cgccaaacac aatattcaca
      301 tcacattttt tattctcggc tgggtggctg agcgctaccc gaatctggtg cggcagatcg
      361 catcggccgg ccatgaaatt gcctcccacg gctatagcca tcagctaatt taccgccaaa
      421 ccccggaggt gtttcgcgct gaaaccctta agtctaagca gattctggag gacattagcg
      481 gccaagcggt gaccggctac cgagcggcca gttactccat cacccgccaa tccctctggg
      541 cgctggatat tttggggcag ctgggtttta cctgggactc cagtattttc cccacccgcc
      601 acgataacta cggcattccc ggcagccccg aggaacccta tgtaatcatc accgatagcg
      661 gcgcccgcct gacagaattt cccctcacca ccgccaaagt gctcggccag cccatcccgg
      721 cggcaggcgg tggttatttt cggcaatatc cctattggct gtcccgctgg ctgtttgcac
      781 gggcgagcga ccaggggcgc aaaagccaga ttttttacct gcacccttgg gagatcgacc
      841 cggagcaacc gcgcgttgcc aatgccagct ggttttcaaa ctttcgccac tacaccaatt
      901 tgcatcgctg cctgcccagg cttgagcgga tgctggaaga ttttgccttt ggcaccatgt
      961 cccaagcact gcaagcggca ccgctgagcc aaaccctcaa acaaagcgac ctgcatagct
     1021 gcggcaagta ggtctaaacc tgaacccctc ggctaatggg agctgaagtg tttagaaaac
     1081 ggcacttgta agcgcgcccc cgcgccacca tcataaatag ctttgctccc tatcatctac
     1141 gaggcacgtt atgcacccag ggcacgaccc catctggtcc gctattttgg cggagacgcg
     1201 ccaagaggcc caaagggagc cagcgcttgc cagcttcctg cacgccacca tcctcaacca
     1261 cgccaccttt gaagcggccc tgagctttca tctagccaac aaactcggca gcactgccat
     1321 ctcctcgctg ttggtacggg agttaatcga acaggccctg caccaggatg ccagcatcgg
     1381 taatgcagcg cgggcggata ttcaggctgt gtttgagcgc gactccgcct gtgaagccta
     1441 cagcctgccg tttttgtatt tcaaaggatt ccatgccctg caggcctata gggtcgccca
     1501 ctggctgtgg aagcagggcc gcaagtccct ggcgtattat ttccaaagcc atatttccct
     1561 ctgtttcggc gtcgatattc acccggcggc gcgcctgggc aaggggctga tgctcgacca
     1621 cggcaccggc attgtgatcg gcgaaacctc cgtagtggac gatgacgtat cgattatgca
     1681 ggcggtgacc ctcggcggca ccggtaaaga aagcggcgat cgccatccca aagtgggcaa
     1741 gggggtatta atcagtgcgg gagcaaaaat tctcggcaac atcactgtag gtgagggcgc
     1801 caagataggt gcaggcagcg tggtattaaa acccgtggcc gcccatacca cggtggccgg
     1861 ggtgcctgca gtagtggtcg gggcgcccac tactcaaagc ccggccaaag atatgatcca
     1921 cgaaatcagt tgcgatgagc tgtgcgcgca acttaagcag caggcggcca aaaactaacc
     1981 caggccgcgc actgccggcg cctttagttg gtcttttgcg cggtaggagc gggtgtggcg
     2041 gaggctttca caagctcttt accctgggtg aaaatgcttt tttcccgctc aaacttatcc
     2101 accacctgct gtttttctgc catccgaatc accatcagat tttccgtgtt cgccagctcc
     2161 gttttggttt tggccaaact atcgagcagg ctgggcggta ccggtttggc ggcccgttca
     2221 aattcggcag ccttaacgcc tatgccgtcg atctgccgct ttaaattatt gatattgccg
     2281 cgcaccaggg cgatgttggc gtccaggcga tccagctggc gcagcttggc gtcttctatg
     2341 tcttgagttg agctgtaacg gcgggagagc tgttcatatt cttctttgat cgcttggcgc
     2401 gcctccaaag ccttgatgtc ctgctcggta agcgcaggcg gcaccacctt aatcacgcgc
     2461 ccggctgcat tgagcacctc atagccgcgc tgggaaaagg ccggtggaat actgtggtcc
     2521 atcaccttca cgccctgatc attcacatag cggtagtagg ccgcccgctc ctctttgctg
     2581 gtgtcagcag gcgccgccga caacacgcca gaaaaacaac tgatgctcaa ccccagagca
     2641 gctaataaat atctcatgcg tcaacaccat aggttttacg gtataactca atgtttgcca
     2701 ccagctccgc ctgagcgggg tcggcggcta aatagctgag gatgtgatca agcttaataa
     2761 tactgacaac cggaacaccg gtgagctgca ccagttctcc tattgcggat aattcacccc
     2821 gaccgcgctc ctgtcggttc aaacctataa cgatagccgc aggctcagca ccggcagcct
     2881 ggataatctc cagcacttcg cgcacggcgg tgcccgcggt aatcacatca tccacaatca
     2941 ccacccggcc cgccaaaggg gcgcccacca gctggccgcc ttcaccgtgg gttttggctt
     3001 ctttgcggtt aaaacaatag ggcacatcgc ggccgtgttg atcagcgagc gccaccgccg
     3061 tggtggtggc aaggggaata cccttatagg ccgggccaaa aatcatgtcg aaacttatgt
     3121 tggcatccac caatgcctgg gcgtagaaac ggcccaactg ggccaaggcg ctgccgattt
     3181 gaaactgtcc ggcgttaaaa aaatacgggc tttggcggcc ggattttagc gtaaaggtgc
     3241 cgaatttcag cgcttggcag gccagtgcca gctgaataaa ttgcgattgg tagggaagca
     3301 tggggactcc aaattgaggc acaggccgtg attttgacat gcggccaccg cagcgggcga
     3361 gaagcagcta ttattgacca gcgccaccgg gtgggacgca gcatggcctg cgcaaaaacc
     3421 ttaacacgat aacaacagag gttccgtcac agtgcatctg ctcgcgcgcc gagcgaccga
     3481 agatgcagct gggccacact gaggtgggcg caagtttacc tgtatttaca cgcagccgtt
     3541 acatccacag ccgcagcacg cttaacaccc tcctcctgca gtgttacaca acaccgcctt
     3601 aggggttgcc gcacctgtgc attattaaca atagttgtaa aagaccggta tgatacacag
     3661 tccgtttcta aggggccatc atgcgcatta tctcactcag tgtcgacggt attcatcagg
     3721 ccgcctctcg cggtttgtat gattggatcg ccaaacaaga cgctgatatt gtctgccttc
     3781 aagacctgcg ggccctggag tctgaactgg accacgcggt atttcatccc gagggttatt
     3841 acagttattt tttcgactcc ggcatcaagc attacaacgg tgttgccatt tacacccgcc
     3901 acgcccccaa ggcgctgatc tatggcttag gctttgccag cggcgtggat atggaagggc
     3961 gttatttgca ggtggatttt gagcgctact ccatcggcac cctcctcgct ccctgcgccc
     4021 aggcggaaaa cgaatccctt gaggtgaagc ttaagttttt tgaagacatg caggcgctga
     4081 tgcacaaaat cacccgcaag cgccggcatt ttatattttg cggcaattgg gccatggccc
     4141 acacccgcaa agatgtgcag aactggcagg aacacataag cacgccgggg tttttacccc
     4201 aggagcaaca gtggctcaac caagcgttta accagctagg ttatgccgat gccttccgcc
     4261 aagtgaataa agatatggac gaattctcct ggtggccctc cggccagatc ggcaaaggct
     4321 gcggctggcg cacggattac cacatcattt ccgaagccct caaacgcaag gtggaatacg
     4381 ccaccattta caaaacccag gaattttcca gccatttgcc ggtggtgatt gactacgata
     4441 tagatgacct agtgtaacgg gcagagacgg cagagggatt gttttgggca gccattacgt
     4501 attgatcgat tatgaaaacg tccaaccgga caacctggcc cagttgaccg acccggcctt
     4561 cagggtcttg gtgtttgtgg gtgataaaca aatcaaaatc gcctttgaat tagccgccgc
     4621 catgcaggcc ctcggcgcgc gggcggagta cgtcaaaatc gaaggcaatg gcctgaacgc
     4681 cctggatttt catatcgcgt tttatatcgg ccagctcgcc actagcgaca aacacagcca
     4741 gttccatatc atttccaagg acacgggttt tgacccgctg attcgccacc tcaaacacaa
     4801 acacatccaa attgagcgcc gggaagagct cacagccctg ctcaaaccca agcctcaaac
     4861 ggtaaaaaca ccgcagccag cagcgcaagc cgcagcgcct gcgccaccgg caaccaatcc
     4921 ggtggccaag gtatttgctt atttgcgcat cgataccaac ccaaggccga aaacacgcac
     4981 ttctttgctt aattttctga attcgcacct aaagcaacag tttgataaaa acgcgttaaa
     5041 cactctggta gatgagctaa tcgatcgcaa aatcattgtg gcggatggcg ataagattag
     5101 ttacccgccg cccaaaccgt cacaaaaggc gccccaggtt taagccctcg ccgattggct
     5161 ctgcaaagat cccctatact gaacctattc accgcaccta agttattgag ctatttatgt
     5221 ccgaccgcgt gctcgatttg cgaagcctcc taaacgacct ccacaaaacc aagcgcattc
     5281 gcctggagga cgttaatttt ttgctgggca gcacccgcac cc
//
LOCUS       DAOQGS010000271         5320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7910_length_5320_cov_4.854701, whole genome shotgun sequence.
ACCESSION   DAOQGS010000271 DAOQGS010000000
VERSION     DAOQGS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7910_length_5320_cov_4.854701"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PKZ52_10795"
     CDS             complement(<1..800)
                     /locus_tag="PKZ52_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60302.1"
                     /translation="MPQAYLSRYAEPEAQAAGLMLAGGKRWRHVLTVPALDEMPAFVE
                     RLAKRSEDALVILVVNQSDSPITPANQLLLDAIHQNYPACTQCGHLSLHHLGALSLLL
                     VDRANTALPPKTGVGLARKIAADIALQLIQQGQVASQWIHCTDADASLPDRYFYLPED
                     HPAVAASLPFRHVGAPGPLLDATLLYERAIRYYAQGLGFAGSSYDFLCLGSALAFKAR
                     AYAQVRGFNLREAGEDFYLLNKLAKLGGIYSPASQNDNTIELAARLSNR"
     gene            complement(800..1393)
                     /locus_tag="PKZ52_10800"
     CDS             complement(800..1393)
                     /locus_tag="PKZ52_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNG60303.1"
                     /translation="MDSQPRPGYTLRYATQPIWTQTVLANLDYFLLDHAAAEKKASGM
                     AMSMVSHYPDKTDIVTAMVDLALEELAHFKQVVKIILERGLILTADTPDHYVNQLRAL
                     HRKGSDVYLLDRLLIGSLVEARGCERFGLIATHLPEGPLRHFYRAICESEARHEDLFL
                     RLAYDHFAAPVVLARLDELLDCEAAIVAALPIRPALH"
     gene            1508..2692
                     /locus_tag="PKZ52_10805"
     CDS             1508..2692
                     /locus_tag="PKZ52_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HNG60304.1"
                     /translation="MIIQPKVRGFICINAHPQGCAANVQEQIAYIQNQPAISGGPKNV
                     LVIGSSTGYGLASRITAAFGCGANTLGVFFERPGTDKKTATAGFYNAAAFDELARAKG
                     LYAESINGDAFSNEIKQQVIDTIKNGMGKVDLVVYSLASPRRTDPVTGEVYQSVLKPM
                     GEAYTTKNLNTDTLKITDVTLDPATDAEVAATVKVMGGEDWELWLKALAEADLLAEGV
                     KTVAYTYLGEKITWPIYGKATIGKAKEDLDRAALALNGLLSQHKGQANVAVLKALVTQ
                     ASSAIPIMPLYLSLLMKVMKEEGSHEGCIEQLYRLFTEGLYTATPRLDGQNRFRMDEK
                     ELAAHVQAKVTELWPQVTESNLFELTDFKGYTAEFLKLFGFGQPSVDYEADTNPEVQV
                     RF"
     gene            2745..3701
                     /gene="lpxL"
                     /locus_tag="PKZ52_10810"
     CDS             2745..3701
                     /gene="lpxL"
                     /locus_tag="PKZ52_10810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HNG60305.1"
                     /translation="MLAKPHFSRQLLHPRYWFTGLGFALWWACAQLPYRWQMALGAGL
                     GRALSRLAPRRRAIAERNIQLCFPALNEQQRAAMVDGVMDSVGKAFFETGMAWFMPHS
                     RLKTLIKLQGLEHLQAAEAQGRGVILLAMHFTHLELGAAAVSMNHSIDGTYRPHANAF
                     YDYIQRKGRERHNATGSVITRGDMRGMLRALRNGRAVWYAPDQDYGREHSVFVPFFNI
                     PAATITATSQIARLGRGLVIPFVHYRLPGGEGYGVDILPPLANFPSQDEEADTQLISQ
                     LVEQEVAKRPEQYLWVHRRFKTRPEGEADLYQAVGIEKGSRT"
     gene            3789..4664
                     /gene="dapA"
                     /locus_tag="PKZ52_10815"
     CDS             3789..4664
                     /gene="dapA"
                     /locus_tag="PKZ52_10815"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNG60306.1"
                     /translation="MFQGSMVALVTPMKADNSLDWDALHKLVDWHLQEGTHGLVPMGT
                     TGESPTLDYKEHMDVVAAVVDQVNGRIPVIAGTGSNSTAEAIELTQAAKDLKADGCLL
                     ITPYYNRPTQEGLYLHHKAIAEAVAIPQILYNVPGRTGVDMTPATVARLAQVANIVGI
                     KEATGDLSRISAMKASCPEDFVLLSGDDASAIAAMELGAVGEISVTANIAPKAVAQMC
                     QAALDGDFAKAHAINQTLSNLHRLLFVEANPIPVKWALNQMGRIDTGIRLPLTPLSAE
                     HHPSLREALKAAGIL"
     gene            4686..>5320
                     /gene="bamC"
                     /locus_tag="PKZ52_10820"
     CDS             4686..>5320
                     /gene="bamC"
                     /locus_tag="PKZ52_10820"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNG60307.1"
                     /translation="MTFVSHHFLVGLCAAALLSGCGIFADHSRDYQRAATIKPLVLPP
                     GVQAQPLEPLYPIPKIAEQEVNLADLGNGSAPRPDPLSKNLEEAKVKIQRVGSDRWIL
                     AEAPSGQIWPLTQSFLAEFGFSLAKTQAAVGLIETNWMEFKDDVGQLHRFTIRIEQGV
                     HPDTTEVHILEQQTAKSANPGNPAWPAESSNLSRENWLVDNLANSQFSRLR"
BASE COUNT         1173 a         1499 c         1536 g         1112 t
ORIGIN      
        1 cggttggaca gccgggccgc aagctcaatg gtgttatcgt tctggcttgc agggctatat
       61 atgcccccga gttttgccaa tttattcagc agataaaaat cctctcccgc ctctcttaaa
      121 ttaaacccgc gcacctgggc gtaagcgcgg gctttaaacg ccagggcgct gcccaagcac
      181 aaaaaatcat aggagctgcc tgcaaagccc aggccctggg cgtaataacg gatggcgcgc
      241 tcgtatagca gggtggcatc caataatggg ccgggggctc ccacatggcg aaagggaagg
      301 ctagcggcta ctgcggggtg atcttcgggc aggtaaaagt agcggtccgg gaggctggca
      361 tcggcgtcag tgcagtggat ccattggctt gccacctggc cctgttggat cagttgcaga
      421 gctatatcag cggcgatttt gcgggctaga cccacgccgg ttttgggtgg cagggctgtg
      481 ttggcgcgat ccactaacag aaggctcagc gcgcccaggt gatgaagaga taagtgccca
      541 cattgggtgc aggctggata gttttggtgg atcgcgtcca gcaaaagttg gtttgccggg
      601 gtaatcgggc tgtcgctttg gttcaccact aagatgacca gcgcgtcttc cgaccgcttc
      661 gctagccgct ccacaaacgc aggcatttca tccagtgcag gcaccgtcag cacatggcgc
      721 cagcgctttc ctccggccag cataagccct gcggcctggg cctcgggttc agcgtagcgg
      781 cttaagtagg cctggggcat tagtgcagtg cagggcgaat gggcagggcg gctactatgg
      841 cggcctcgca atccagcagt tcatccagcc gagcgagcac cacgggggct gcaaagtgat
      901 cgtaggcgag ccgcaggaag aggtcctcat ggcgggcttc ggattcacaa atagcccggt
      961 aaaagtgccg caggggtccc tcgggcaggt gggtggcgat gaggccaaag cgctcgcagc
     1021 cgcgagcttc caccaggctg ccgatcagca atcgatccag caggtacaca tcgctgccct
     1081 tgcggtgcag ggcgcgcagt tgattgacat agtggtcggg ggtgtcggcg gtgaggatca
     1141 ggccgcgctc taggatgatt ttgaccacct gtttgaagtg ggccaactcc tcaagggcta
     1201 aatccaccat ggcggtgact atgtcggttt tatccggata gtgggacacc atggacatgg
     1261 ccatgccgga ggcctttttc tccgccgctg cgtgatccaa caaaaagtaa tcgagattgg
     1321 cgagcactgt ctgggtccaa atcggctggg tggcatagcg cagtgtgtaa cctgggcggg
     1381 gttgggaatc catggcacaa cttcttaacg aatggggcgc gcattatagg ccgggtgcaa
     1441 aaagagtcga tattttgccg ctgctcttta taatgcgcgg ctttatacct gctcttacgg
     1501 tgcttcgatg attattcaac ccaaggttcg cggtttcatt tgtattaacg cccacccgca
     1561 aggctgtgcg gccaatgtgc aggagcaaat tgcctatatt caaaaccagc ccgccataag
     1621 tggcggcccg aagaatgtgt tggtgatcgg cagttccacc ggttacggcc tggcctcgcg
     1681 catcacggcg gcctttggtt gcggggcgaa tactctgggg gtgttttttg agcgccccgg
     1741 caccgataaa aaaaccgcta cagcgggctt ttacaacgcc gccgcgtttg atgagttggc
     1801 ccgcgccaag gggttgtacg ccgagagcat caacggcgat gcgttttcaa acgagatcaa
     1861 acaacaggtt atcgacacca ttaaaaacgg catgggcaag gtggacttgg tggtctattc
     1921 cctcgcctca ccccgccgca cggaccctgt gaccggtgag gtgtaccaaa gtgtgttaaa
     1981 gcccatgggt gaggcctaca ccactaaaaa cctcaatacc gatactttga aaattaccga
     2041 tgtaaccctg gacccagcca ccgatgccga ggttgccgcc acggtgaaag taatgggcgg
     2101 cgaagactgg gaactatggc ttaaagccct ggctgaggcc gatttgttgg ctgaaggggt
     2161 gaaaaccgtc gcttatacct acttgggcga gaaaatcacc tggcccattt acggcaaagc
     2221 caccattggc aaagccaaag aagatctgga ccgcgctgct ctggcactca atggactgct
     2281 cagccagcat aagggccagg ccaatgtggc ggtgcttaaa gccctggtga cgcaagcgag
     2341 ttcggctatt cccatcatgc ctttgtacct ctcgctgctg atgaaagtga tgaaagaaga
     2401 gggcagccat gagggctgta tcgaacagct gtaccgcttg ttcactgaag gcctctatac
     2461 ggccaccccc cgtttggacg gccaaaaccg ctttcgcatg gatgaaaaag agctggccgc
     2521 acatgtgcaa gctaaggtga ccgagctttg gccccaggtg actgaaagca atctgtttga
     2581 gctgaccgac tttaaaggct acaccgccga gtttttaaaa ctgttcggtt ttggtcagcc
     2641 gagcgttgat tacgaggcgg ataccaaccc tgaagtccag gtgcgcttct agggcgagcc
     2701 ttttaggttt tgttgagcgt acagactgtg ctgctactta gcctatgcta gccaaacccc
     2761 attttagccg gcagctgctg cacccgcgct actggttcac cggtttgggg tttgccctat
     2821 ggtgggcctg tgcgcaattg ccctatcgct ggcagatggc gttgggcgca ggcctgggcc
     2881 gggccttaag ccgcttggcc ccccggcgcc gagctattgc cgagcgcaat atccaattgt
     2941 gcttcccggc attaaatgag cagcagcgcg cggccatggt agacggcgtg atggattcag
     3001 tgggtaaagc cttctttgaa accggcatgg cctggtttat gccccacagc cgattaaaaa
     3061 ccttgatcaa attgcagggg ttggagcatc tacaggcagc cgaggcgcaa ggccggggtg
     3121 tgattttact ggccatgcac ttcacccatt tggaattagg ggcagcggca gtgagcatga
     3181 atcactccat cgatggcacc tatcgcccac acgccaatgc cttctacgac tacatccagc
     3241 gcaagggccg cgagcgccat aacgctaccg gcagtgtgat cacccggggc gatatgcgcg
     3301 gcatgctgcg cgccctgcgc aatggccgcg ccgtctggta cgcgccggat caggactatg
     3361 gccgcgagca ctcagtattc gtgcccttct ttaacattcc tgctgccacc attaccgcca
     3421 ccagccaaat cgcccgttta ggccggggtt tggtgattcc attcgtgcat taccggctgc
     3481 ccggtggcga gggctatggt gtagatatac tgccgccttt ggccaatttc ccaagccagg
     3541 atgaagaggc ggacacccaa ttgatcagcc agttggtgga gcaagaagtg gccaaacggc
     3601 ccgagcaata tctgtgggta caccgccgct ttaaaacccg gccagagggg gaggcggatc
     3661 tgtaccaagc ggtgggaata gaaaagggta gccgcaccta gcttggggaa ttgcgccctt
     3721 gttctaccac cgccccttaa gtaacatggc gcccctttat ccttcagcag gcaacctggg
     3781 agaagcttat gttccaaggc agtatggtgg ctctggtcac cccgatgaaa gcggacaact
     3841 cgctggattg ggacgccctg cataagctgg tggattggca tctgcaagaa ggtacccacg
     3901 gccttgtgcc tatgggtacc accggcgaat cccccaccct cgattacaaa gaacatatgg
     3961 atgtggtggc ggcggtagtg gaccaggtga atggccgcat cccggtgatc gcaggcaccg
     4021 gttccaactc taccgctgaa gccatcgaat taactcaagc ggctaaagat ctaaaagccg
     4081 atggctgcct tcttatcacg ccttattaca accgtcccac ccaagaaggc ctgtacttgc
     4141 accacaaggc catcgccgaa gcggtagcca tcccccagat tctctacaac gtcccaggcc
     4201 gcacgggtgt cgatatgacc cctgccactg tggcgcgcct ggcgcaggtc gccaatatag
     4261 tcgggatcaa agaagcaacg ggcgacttaa gccgaatcag tgccatgaag gcaagctgcc
     4321 cagaggattt tgtgctcctg tcgggggatg atgccagcgc catagcggcc atggaacttg
     4381 gcgcggtggg cgaaatctct gtgaccgcga atatagcccc caaagccgta gcgcagatgt
     4441 gccaagcggc cttggatggg gattttgcca aggcccatgc tattaaccaa acccttagca
     4501 acctgcaccg tttgttattc gtggaagcca accccattcc cgtcaaatgg gccctcaatc
     4561 aaatgggccg tatcgatacg ggcattcgct tgcctttaac gccgctgtcg gcagaacacc
     4621 acccatcatt gcgcgaggcc ctcaaggctg cgggcatttt ataaattaac tggcgatacc
     4681 ccttcatgac gtttgtctcc caccattttc ttgtcggcct gtgcgcggca gcgcttttga
     4741 gcggctgcgg tatttttgcc gatcacagcc gcgactatca gcgcgctgcc accatcaaac
     4801 cgcttgtgtt gccccccggc gtgcaggctc agcctttaga gcctttgtat cccatcccca
     4861 aaatcgccga acaggaagtt aacctcgccg acctcggcaa tggctcggcg ccgcgcccgg
     4921 acccattgag taaaaacctc gaagaagcca aagtaaaaat ccaaagagtg ggcagtgacc
     4981 gctggattct cgccgaagcc cccagcggac aaatttggcc cttaacccaa agcttcttgg
     5041 cggaatttgg ctttagcctg gcgaaaaccc aagccgctgt aggcctgatt gaaaccaact
     5101 ggatggaatt taaagacgat gtgggccagc tgcaccgttt caccattcgc attgaacagg
     5161 gcgtgcaccc ggataccacg gaagtgcata ttctcgagca gcaaactgcc aaaagcgcca
     5221 accccggcaa ccccgcttgg cccgctgaat cgagcaatct cagccgggaa aactggctgg
     5281 tggataacct ggccaacagc cagttttccc ggctgagatt
//
LOCUS       DAOQGS010000272         5294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_7961_length_5294_cov_5.498568, whole genome shotgun sequence.
ACCESSION   DAOQGS010000272 DAOQGS010000000
VERSION     DAOQGS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7961_length_5294_cov_5.498568"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PKZ52_10825"
     CDS             complement(<1..212)
                     /locus_tag="PKZ52_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60308.1"
                     /translation="MLLAPHRLALLACAIAALSLTGCGSKGKSRSTSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSS"
     gene            1515..2468
                     /locus_tag="PKZ52_10830"
     CDS             1515..2468
                     /locus_tag="PKZ52_10830"
                     /inference="COORDINATES: protein motif:HMM:NF033581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNG60309.1"
                     /translation="MGSLNDLFDTLHSTYGKPSIPPERLLRASFLQVLYTLRSERQLC
                     EHINYNMLYRWFVGLELEDVMWDHSTFSKNRDRLLQQDVIPEFFDQVLQIARERKLLS
                     EDHFSVDSTLIDARASHKSFRLKDDDAPQSGSTEGNFHNETRSNRTHQSTTDSDAELM
                     RKPKGKEARLSYGVHHIIENRNSLMVGVKTTRAASVSEREVAEDLLAELSGEQRKTIG
                     ADKGYDIADFVDTCRQYKITPHAVQNDTRLGGSSIDNRTTRHVGYQVSMQNRKMIEAT
                     FGLIKQYGGNRRMMYRGFERANPAVIFSVSVFNLLRIRNAE"
     gene            complement(2614..3069)
                     /locus_tag="PKZ52_10835"
     CDS             complement(2614..3069)
                     /locus_tag="PKZ52_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60310.1"
                     /translation="MGKTTELNRLVKELNGDETTAAFYCDVSAYLNLNDPDVSLAELL
                     MAVLTGLSDAVKREFDSDILKDSIWDRVKSTMNSTVTLAPKIKAGTVEASISLHENPE
                     FKKQLREFTKDSSSFIQAARTFAQDIASAIRKQTGTAKLVLAVDSLAAC"
     gene            complement(3126..3266)
                     /locus_tag="PKZ52_10840"
     CDS             complement(3126..3266)
                     /locus_tag="PKZ52_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60311.1"
                     /translation="MSNNQSLLKSFFNALPLAEKPGVYLGDQLSTAPLTRMKMPLHRS
                     SN"
     gene            complement(3344..>5294)
                     /locus_tag="PKZ52_10845"
     CDS             complement(3344..>5294)
                     /locus_tag="PKZ52_10845"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HNG60312.1"
                     /translation="APTAVVSSTTRPVPLVFRYEERDLLETTVMNLVEAKQAPVYVVH
                     FTQNDAAQTTQNLMSQNYCSSEEKAAIAKALRGVKFTSPYGKDFKRFLSHGIGLHHAG
                     LLPKYRVLVERLAQLGLLKIICGTDTLGVGVNVPIRTVLLTRLSKYSGSKLALLTARD
                     FHQITGRAGRKGFDDIGYVVAMAPEHVIENNRLEAKAAGDPKKAKKLVKRKPPEKFVA
                     WSNDTFTQLQAAPPEPLVSRFEVSHGMLLQVLSRKTDGCRAMQQIIKDSHETAAQKKS
                     HRKRAWQLFRSLHERNIIEILPKSERSPVGAKLRLNIDLQEDFSLNHALSLYLMDTLE
                     LLNPESPEYALDMLTLIESILENPDIILRRQLDALKTEKMAEMKQQGIEYDERIARLE
                     ELEYPKPQREFIYLTFQNFALKHPWVGQENIRPKSIVREMVENYVDFAGYIKLYDLER
                     SEGLLLRHLSSVYKVLAQTVPPKFKNADVQEVEDWLAGILHGTDSSLLDEWERLKNPD
                     WTPKEDDADAAVTEVDITKNKREFTALIRTEIFHFLRAMASGKYEAAAALVPPWSAEA
                     LSEKMQDYRQEHGGINLDNDARNLRNTYITADENAGVWSVAQVLIDPDALNDWQAVFS
                     VDKARAKEEGKPSLQLLGLGPIVED"
BASE COUNT         1358 a         1219 c         1297 g         1420 t
ORIGIN      
        1 gaacttgatg aactgctgga cgagcttgag ctgctagagg aactggaact ggaggaggaa
       61 ctgctgctgg aggaactgga acttgatgag ctggaacttg aagaactgct ggagctagtg
      121 ctgcgggact tacctttact gccgcagccg gtaaggctaa gggcggcgat ggcgcaggct
      181 aacagggcta ggcggtgagg ggctaataac atgatgcgtt tttcctttga caaggtgggt
      241 tatgtggtct acagcctcgg gcaggcatta tgccagaggc tgggcagaaa ctgtacggcg
      301 cgggctcaca aggtatatgc acgatggagc acaagcccta ggggtaatct cgcacagggg
      361 ctctaaaacg gacattggcc taacctatgt gcaggtgcgg catttttacc aggttaagag
      421 ggcttttgca tgggttttca caggagtgaa accggttaaa tggtgttaat ttttagcggc
      481 ttgagtggcc cagctatagg ccaggggtat agtcacaatg accaaacagg aatttgtttg
      541 cctactagta agctctgtca atccacagat acagcttccg atgcgttaaa accttagggg
      601 ttattttgcg catgatttta aaagagtatt ggtgttgtta atacttgcaa atagcccgtt
      661 gagatgcttt tttaaaatat tgcgaattta cttataaaat atctcgcttg cgcagcttgg
      721 cgaaaaatat cgactggaca ggtatagggt gagataaccc agcatgtcgt gatatcagat
      781 cgaacatgct gcctaagggc aagttcgcct gcaactttcc caaacagcta aatacaagtg
      841 ggaaacccta aatcaacata aaattatcgg tcacaattta agacgctaat tttttttggg
      901 ggggactaaa ttttcaatga acatttgaat agattcacca ggaactttta caccaaacat
      961 tgtgcaacaa gggtataaaa atcacccaca ctcaatacaa aattattgaa catttacttc
     1021 ccttgctgca gggccgcaag tttgcacaac ttttccctac ttaaaggttt taaatgccat
     1081 ttttcacatc gcagaacatg gctacaaaag tgatgacttc agccctattt cggcgattgg
     1141 cacacagtat acggcgcgga tcgctgggcc agaccggcgt actggaaaat atttctgccg
     1201 catcgcaaga agcagatgtc attaatattc acgttgccgc atctacttgt atagcagact
     1261 gttaaagcac ccccagacag aatacgagca ttaaaaaacg gtccgcaatc gattggcaaa
     1321 tctaacagct tgttgaatta ttcgataaca agcctcaacg cagctaaaat ggcgttgttc
     1381 tcccgtccga taatgaaaaa tgcaatgcgt ggaatcgaca tgagccaacc atcgttattc
     1441 atttgcagaa ccgtgcaaga ttttgcaccg aaagatcatc cgctgagaga ccttagagag
     1501 ctaattgctc ggtattgggc tcgttaaatg accttttcga tacactccat tcgacctatg
     1561 gaaaaccttc gattccgcca gagcgattac tgcgcgcctc ctttttacag gttttataca
     1621 cgcttcgtag cgagcgacaa ttgtgtgagc acatcaacta caacatgctt tatcgctggt
     1681 ttgtgggcct agagttggag gacgttatgt gggatcactc tacatttagc aaaaatcgtg
     1741 atcgattact tcagcaagat gtcattccgg agttttttga ccaagtattg cagatagctc
     1801 gagaaagaaa attattgtca gaagatcatt ttagcgtgga cagcacgctg attgatgcgc
     1861 gggcatcaca caaaagtttt cgccttaaag atgacgatgc ccctcaatct ggcagtacag
     1921 agggaaattt tcataacgaa acgcgcagca atcgcacgca tcagtcaacg actgattccg
     1981 atgccgaatt gatgaggaag cccaaaggta aagaagcgcg gctgagttac ggagtgcatc
     2041 acattatcga aaatagaaac agcttgatgg tcggggttaa aacgacgcgc gccgcgtcag
     2101 tgtctgaacg cgaggtggca gaggatttgc tggcagaatt gtccggcgaa cagagaaaga
     2161 ctatcggggc cgataaaggg tatgacatag ctgattttgt agatacgtgt cgacaataca
     2221 agattacccc gcatgcagta caaaatgata cccgcctagg cggatcgtca attgataata
     2281 ggaccactcg tcacgttggc tatcaagtga gcatgcagaa tcgaaaaatg attgaggcta
     2341 catttgggtt gattaaacag tacggcggca atcgacggat gatgtatcgg ggctttgagc
     2401 gagcgaatcc agcggttatt ttttcagtaa gcgtgtttaa tttacttaga attcggaatg
     2461 ctgaataaaa aacactcagc caaccgctaa ataccgtaaa aaatgatgtg accgagccgt
     2521 ttttaaattc ctgtgtcaaa tttttctcaa tgtgaaattt aaacatctac ccattaaata
     2581 tttggtgtgt taccaaaaaa atattgctct atttcaacaa gctgctagag aatccaccgc
     2641 taaaaccagt ttagctgtgc ccgtctgctt gcggatggcg ctggctatat cctgggcaaa
     2701 tgtgcgcgca gcctgaataa aactgctgga gtctttggta aattcacgca gttgtttttt
     2761 aaattccggg ttttcgtgca ggctgatact ggcttcaact gtgcctgcct tgattttggg
     2821 ggcaagcgtt acagtggaat tcatagtgct ttttacccga tcccaaatgg agtcttttaa
     2881 aatatccgag tcaaactcgc gcttaacggc atcggacaag cctgtcaaca ccgccattaa
     2941 taattcggcc aggctcacat ctgggtcatt gagatttaaa taagcgctga catcacaata
     3001 aaaagctgca gtggtttcat cgccattaag ctctttaact agacgattta attcggtagt
     3061 tttgcccacg ccgcgcaggc cggaaaataa aaaagacttg cccccggagg cgtcactcca
     3121 gtcaattaat tgcttgatct gtgcaacggc atcttcatcc gggttaatgg cgcggttgac
     3181 aattgatcgc ccagatacac gccgggcttt tcagcaaggg gcagagcatt gaagaaactt
     3241 ttcagcaggc tctggttgtt agacataacc gaatttcgtg tgtggcagcg gatttacgca
     3301 gcacagccct tgtgttttcc agttaacatg ggttgagctg gggctaatcc tccacaatcg
     3361 gccccaaccc cagcaattgc aaactcggct taccttcttc tttggcccgt gccttatcca
     3421 cactaaatac cgcctgccaa tcgtttaacg cgtctggatc aattaatacc tgggctacgc
     3481 tccaaacccc ggcattttcg tctgctgtta tataggtgtt gcgcaaattg cgtgcgtcgt
     3541 tatccaagtt aataccgccg tgctcctggc ggtaatcctg cattttttca cttaaggctt
     3601 cggcgctcca gggcggcact agggcggctg cggcctcata tttgccgctg gccatagcgc
     3661 gcaggaagtg gaaaatttcc gtgcggataa gggcagtaaa ttcccgttta tttttggtga
     3721 tgtccacttc cgttacggca gcatccgcgt cgtcctcttt gggcgtccaa tcggggtttt
     3781 ttaagcgctc ccattcatcc aataaactgc tatcagtgcc gtgtaaaatg cccgccaacc
     3841 agtcttcaac ctcctgcacg tctgcgtttt taaatttcgg cggtacggtt tgcgccagca
     3901 ccttatatac gctggagagg tgcctaagca gcaggccttc gctgcgttct aaatcgtaga
     3961 gtttgatgta gccggcaaag tccacataat tttccaccat ttcccgcact atgcttttgg
     4021 ggcggatatt ttcctggccc acccaggggt gttttaaggc gaagttttgg aaggttaaat
     4081 aaataaattc ccgctggggc ttggggtatt ccagttcttc taaacgcgca atgcgctcat
     4141 cgtattcaat accctgttgt ttcatctccg ccattttttc ggtttttaag gcatccaatt
     4201 ggcggcgcag aatgatgtcg gggttttcta aaatcgattc aatcagcgtg agcatatcca
     4261 aggcatactc agggctttcg gggttcaaca gttcaagcgt gtccattaaa tacagagaca
     4321 acgcgtggtt aagcgagaag tcctcctgta aatcgatatt taagcgcaat ttagcgccca
     4381 cagggctgcg ctcacttttg ggcagaattt caataatgtt gcgctcgtgc agtgagcgga
     4441 ataattgcca ggcgcgtttg cggtgggatt ttttttgcgc ggcggtttcg tggctgtctt
     4501 taataatctg ctgcatagcg cggcagccat cggttttgcg gcttaatact tgtaataaca
     4561 tgccatggct cacctcaaag cgggatacca aaggctccgg cggcgcggct tgtaattggg
     4621 tgaaggtgtc gttcgaccag gcgacgaatt tttccggtgg tttgcgttta accagctttt
     4681 tggctttttt cggatcgcca gcggctttgg cttccaggcg gttattttca atcacatgct
     4741 ccggcgccat ggccaccaca tagcctatat catcaaaacc tttgcgcccg gcgcgcccgg
     4801 tgatttgatg aaaatcccgc gcggttaata aggccagttt cgagccgctg tatttactta
     4861 agcgggttaa taacacagtg cgaatgggca cgtttactcc cacccctagg gtgtcggtgc
     4921 cgcaaattat ttttaataaa cccagttgcg ccaaacgctc gaccaatact cggtatttgg
     4981 gcaataaacc ggcgtggtgc aggccgatgc cgtggcttaa aaagcgctta aaatccttgc
     5041 cgtaggggct ggtaaattta acaccgcgca gggctttggc gatggcggct ttttcctcgc
     5101 tgctgcaata attttggctc attaaattct gcgtggtttg cgccgcgtca ttttgcgtaa
     5161 agtgcaccac atatacaggt gcctgtttcg cctccactaa attcatcacg gtggtttcca
     5221 ataaatcccg ctcttcatag cgaaatacca agggcaccgg ccttgttgta gaggacacca
     5281 cggcggtggg ggcg
//
LOCUS       DAOQGS010000273         5275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8011_length_5275_cov_4.308621, whole genome shotgun sequence.
ACCESSION   DAOQGS010000273 DAOQGS010000000
VERSION     DAOQGS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8011_length_5275_cov_4.308621"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1341)
                     /locus_tag="PKZ52_10850"
     CDS             complement(133..1341)
                     /locus_tag="PKZ52_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HNG60313.1"
                     /translation="MKIVSAKVIITCPGRNFVTLKIMTDQGVYGIGDATLNGREKAVV
                     SCLEDYLLPQLIGRDASRIEDIWQYFYKGVYWRRGAIGMTALAAIDTALWDIKAKAAN
                     MPLYQLLGGRSRDHIMVYTHANGKNLEDTLLAVEKAKNKGYQAIRVQCGIPGINKTYG
                     VAKGANSYEPADADLPSEEIWSTRKYLDYIPEVFAAVRKEFGNDIHLLHDVHHRLTPI
                     EAARLGKELEPYHLFWLEDAVPAENQAAFKLIREHTTTPLAVGEIFNSIHDCRELIQN
                     QWIDYIRSTVVHAGGITHLRRIADFAALYHVRTGCHGATDLSPVCMGAALHFDYWVPN
                     FGIQEHMAHSEQMESVFTRAYEFNNGFFTPGELPGHGVDINEAEAAKYPYKRACLPVN
                     RLEDGTLWDW"
     gene            complement(1490..2716)
                     /locus_tag="PKZ52_10855"
     CDS             complement(1490..2716)
                     /locus_tag="PKZ52_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNG60314.1"
                     /translation="MHKFTVWAPAPRTARVKLALGNWAPAGELDFASAGCTFRYLQSW
                     LQTDWAYSLDPVHLPLAPAIYYPKTQSQLLPLFANLIPAGRAALLLKNALKLDPLRTL
                     PAEFKLMPGGLNPIRLSLGQQPLGELNLPAWEELENYCQKDREQIGYNYPLARFTDYM
                     AGGCWRGERPKLTVRGSDKLWLAKWQLKDEAINWPQIEYACLELLQQAGLSVPQRHLI
                     PTDACGWLYLIERFDQLANNPVYFISAEVLLGQPKAASVDGDARRDPGSYIALARCVR
                     KYSSNAKADLLELYRRLLANIMLNNTQDHLHKFGLVFHLNKRQWRLAPCFGLRPNSVQ
                     TAHQAMAMGTHGRLRSLANALSLAAEFALTPGQAQQLLAQLRDDLAHWQAVFDNCGVS
                     GRDRQWLGKVIQLGDF"
     gene            complement(2709..3029)
                     /locus_tag="PKZ52_10860"
     CDS             complement(2709..3029)
                     /locus_tag="PKZ52_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG60315.1"
                     /translation="MSQDLSLTEQTASYLTCLGLNIKDARKARKWSQAEVAQRALMSR
                     LTYAQIEKGAPQVQIGLYLRVLELFGLAEPIRHIAAPHLDEEGRRLRAQKNKTNKNKA
                     NKGA"
     gene            complement(3299..4696)
                     /gene="pabB"
                     /locus_tag="PKZ52_10865"
     CDS             complement(3299..4696)
                     /gene="pabB"
                     /locus_tag="PKZ52_10865"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNG60316.1"
                     /translation="MPQIALSIISIPYPANSLEYASRFARSTGFIWLDSGTRREYCGQ
                     YDLISAYPSQHLSAQSATHIDISQGGITRQVTLQEAERWCEEYSRPKNTEQADQAVAF
                     SGGLLGYIGYEWNHPKFKLAASNSLATPLVYLGVYEWALVIDHEQHTAYFVFTENISA
                     QHLQQIMAQFNAPAEYQSSLDTGEFYCGEFKPQTPKSQYLQDINTILDFINAGDCYQV
                     NYSQAFSAQFSGDAFAAYCQLRSGCPGPFSAFISTAEGAILSLSPEQFIQIDTANQAT
                     SRPIKGTAARHKNTQQDGSLARELHRSVKNRAENLMIVDLLRNDFSKNCRPGSVKVPE
                     LFGLYSFTNVHHLISTIEGSLNDKVSHWHFIIDAFPGGSITGAPKKRAMEIIHQLEPH
                     QRHIYCGCIGYWSRNGNTRTNIAIRTLLIEGEQIRLWGGGGIVADSNAEDEYQESLDK
                     IAIFKSILTRSGEPQ"
     gene            4898..5206
                     /locus_tag="PKZ52_10870"
     CDS             4898..5206
                     /locus_tag="PKZ52_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNG60317.1"
                     /translation="MFVPLAKLIDLHDGFRQTHSLAGKSYLLLQTAGQVYLVENRCPH
                     MDAPLANGDIEQGTIRCKAHGIAFDLASGKAQGPLAGSLDCLQRLELVYDGDRIGVEL
                     "
BASE COUNT         1303 a         1246 c         1333 g         1393 t
ORIGIN      
        1 ggaggccatt tttccatctg gcgcgggggt tttacacaat tctacgaaga gccatcttaa
       61 acgatggctg ggcattggtc cggaaaattg gtaccactat acacatcgca cagcaattct
      121 cctttcccgg agttaccaat cccacaaagt accatcttcc aagcgattca ctggcaaaca
      181 ggcgcgttta tatgggtatt tagcggcttc ggcctcatta atatccacgc cgtggcccgg
      241 caattcaccc ggcgtaaaaa agccattatt aaattcatag gcgcgggtaa atactgattc
      301 catttgttca ctgtgggcca tatgttcctg aattccaaaa ttcggcaccc aataatcaaa
      361 atgcaaggcc gcccccatac acacaggcga taaatccgta gcgccatggc agccggtgcg
      421 cacatggtac agggcggcaa aatccgcaat gcgccgcaga tgggtaatgc cgcctgcatg
      481 cactacggtt gaacgaatat aatcaatcca ttggttttgg attaattccc ggcaatcgtg
      541 aatgctgtta aaaatttccc cgaccgctaa gggtgttgtg gtgtgctcgc gaattaattt
      601 aaacgctgct tgattttccg caggcaccgc atcttccaac caaaataaat ggtagggctc
      661 cagctctttg cctaagcgcg ccgcttcaat gggggtaagg cggtgatgta catcgtgtaa
      721 tagatgaatg tcattgccaa attctttgcg cacagcggca aaaacttcag ggatataatc
      781 taaatatttg cgggtggacc aaatctcttc tgaggggaga tcagcatcgg caggctcata
      841 actattggcc cccttggcaa cgccgtaggt tttattaatg cccggaatgc cacactgcac
      901 gcgaatagct tggtaacctt tatttttggc tttttctacg gccaacagtg tgtcttcaag
      961 atttttacca ttggcgtggg tgtacaccat tatatgatcc cggctgcgcc caccgagtaa
     1021 ttggtacagt ggcatattgg cggctttggc tttaatatcc cacagtgccg tatcaatggc
     1081 ggccagggcc gtcataccaa tagcgccgcg ccgccagtac acacccttat aaaaatactg
     1141 ccaaatatct tcaatgcgag atgcatcccg gccaattaat tgcgggagta aatagtcttc
     1201 taagcagctg acaacagctt tttcccgacc atttaaggtg gcatcgccta tgccgtatac
     1261 gccttggtca gtcataattt taagcgtgac gaaattgcgc ccagggcagg tgatgataac
     1321 tttggcggat actattttca tgaagggtcc aaacgaaata gggtgagcaa aaacctgggt
     1381 gaaattagct gatttaaaag ctttttacca ggtgaaactg cagaatttgt gttgcaaaat
     1441 ggtcttacta aatagtggct gctgcctact ggggtatgca acgggcgagt taaaaatccc
     1501 caagctgaat caccttgcca agccactgcc gatcccggcc actcacgccg caattgtcaa
     1561 acactgcctg ccagtgcgct aaatcatctc gcagctgggc tagtaattgt tgtgcttgcc
     1621 caggggtgag cgcaaattca gccgctaagg agagagcatt ggctaaactg cgcagccgcc
     1681 cgtgggtgcc catggccata gcctggtgtg cagtctgtac agagtttggg cgcaggccaa
     1741 aacacggggc caggcgccat tggcgcttat tcaagtgaaa tacaagacca aatttgtgca
     1801 gatgatcctg cgtgttattg agcataatat tagctaataa gcggcgatag agttcgagca
     1861 aatctgcttt ggcgttgctg gaatatttgc gcacacagcg ggcgagggca atgtaactgc
     1921 cagggtctcg ccgcgcgtcg ccatcaacac tagctgcctt gggttggccc aataaaacct
     1981 cagcggagat aaaatacaca ggattattgg ccagctgatc aaagcgctcg attaaataaa
     2041 gccaaccgca tgcatcggtg gggattaaat gccgctgggg cacacttaat cccgcttgct
     2101 gcaataactc taaacaggcg tattcaatct gtggccaatt aatcgcttca tcctttagct
     2161 gccattttgc caaccagagt ttatcgctgc cgcgcacggt taatttaggc cgctctcccc
     2221 gccagcaacc accggccata tagtcggtaa aacgcgctaa aggatagttg taaccaattt
     2281 gttcgcgatc tttttggcag tagttttcta actcttccca tgcaggtaga tttaactcac
     2341 ccaggggttg ttgccctagg cttaatcgaa tgggatttag cccgccgggc attaatttaa
     2401 attctgcagg cagcgttcgc agtgggtcta attttaatgc gtttttgagc aataaggccg
     2461 ccctaccggc ggggattaaa ttggcaaaca ggggcaataa ttgggattgc gttttggggt
     2521 aataaattgc cggggcaagt ggtaaatgaa ctgggtctaa actataagcc caatcagttt
     2581 gcagccagct ttgcaggtag cgaaaggtac aaccggctga tgcaaaatct aattcccctg
     2641 cgggagccca gttgccaagg gcaagtttaa ctctcgcggt cctaggtgca ggtgcccaaa
     2701 cggtaaactt atgcaccctt attggccttg tttttattgg tcttgttttt ctgtgcgcgc
     2761 agacggcggc cttcttcatc taaatggggg gcagcgatat ggcgaattgg ctcggccagg
     2821 ccaaataact ctaaaacccg caaataaagg cctatttgta cttggggggc gcctttttca
     2881 atttgcgcat aggtcaggcg ggacataagc gcccgctggg ctacctccgc ttgcgaccat
     2941 ttgcgcgcct tgcgggcgtc tttaatattt aaacctaggc aggttaggta gctggccgtt
     3001 tgctcagtaa ggcttaaatc ttggctcata tgtatgctgt agcgatctta tgaatttatt
     3061 ttgctaacca taacatacat tttaaaactg aggaatttgc cgtataactc aagccttata
     3121 aaattgggcg atcacaaagg aggccaaccg cgggggcatc agatctatgg gtattttcga
     3181 aatgggcgca ggcacaaagc ccgaggctta agctgcttgg ggccgcttga atgtttccac
     3241 gctagtgccg aatttagact taaacagaga gtaaacctag gtgttttaag ctccaaattt
     3301 attgtggctc acctgagcgg gtaagaatcg atttaaaaat ggcaatttta tccaatgatt
     3361 cttggtattc atcctctgca ttcgagtcgg ccactatacc gccgccgccc cataatctta
     3421 tctgctcgcc ttcaatcaac agggtgcgaa tggcgatgtt ggtgcgggtg ttaccgttgc
     3481 ggctccaata gccgatgcaa ccacaataaa tgtgtcgctg gtggggttct aattggtgga
     3541 taatttccat agcgcgtttt ttaggggcgc cggtaatcga gccgccgggg aatgcatcaa
     3601 taataaagtg ccagtggctc accttatcgt ttaatgaacc ttcaatagtg ctaattaaat
     3661 gatgcacatt ggtgaagctg tataaaccga ataactctgg cacttttaca ctgcccggcc
     3721 gacagttttt actaaaatca ttgcgcagca ggtctacaat cattaaattt tcagcgcggt
     3781 ttttcacgct gcggtgcagt tcacgggcta agctaccatc ttgttgggtg tttttatggc
     3841 gcgcggcagt gcctttaatt gggcggctgg tggcttggtt agcagtatca atttgaataa
     3901 attgttcagg ggagaggctt aaaattgcgc cctcggcggt gctaataaaa gcgctaaacg
     3961 gcccggggca gccgctgcgc aattggcaat aggcagcaaa ggcatcaccg ctgaattgag
     4021 cgctaaaggc ctgagagtaa ttgacctggt aacaatcccc ggcgttgata aaatcgagga
     4081 tggtattgat atcctgcaga tactgggatt taggtgtttg gggtttaaat tcaccgcagt
     4141 agaattcgcc agtatccaaa ctgctttgat attcggcagg tgcattaaat tgcgccataa
     4201 tctgctgcag gtgttgcgct gaaatatttt cagtaaaaac aaaataagcc gtgtgttgtt
     4261 cgtggtctat aaccagtgcc cattcatata cacctaaata caccagtggc gtggccaggg
     4321 aattactggc ggctaattta aatttagggt gattccactc atagcctata tatcccagca
     4381 gaccaccgct aaaggccacc gcttgatcgg cctgctcagt atttttgggg cggctgtatt
     4441 cttcgcacca acgctcagct tcttgcaggg ttacttggcg ggttatgccg ccctggctta
     4501 tgtcgatgtg tgtagcgctc tgggcgctta agtgctggct ggggtaggcg ctaattaagt
     4561 cgtactggcc gcaatattcc cgccgagtgc ccgaatccaa ccaaataaat cccgtgctac
     4621 gggcaaaacg gctggcatat tccaggctat tggccgggta ggggatgctg ataatactta
     4681 gggcaatttg gggcactagg gttttaatcc gctaaaaccg gcattttcgc ataaccgtgc
     4741 agcctgcgtt aatagtgctg gcgtgtaatg gcctgccttg ggctcccacc tgaggaatca
     4801 ctataatcag ctgccaattg tgggatgaat ttctggccgt attggcaaag cttcgttagt
     4861 attactcgac tgccttgatg ttgcttagga gcccagtatg tttgtgcctt tagccaaatt
     4921 aatcgattta cacgatgggt tccgccaaac gcacagcctg gcaggcaaat cctacttgtt
     4981 gctgcaaacg gcagggcagg tgtatttagt ggaaaatcgc tgccctcata tggatgcccc
     5041 cctggccaat ggggacatag aacagggtac aattcgctgc aaagcccatg gtattgcctt
     5101 tgatttagcc agtggcaagg cccaagggcc tctggctggc agtttggatt gtttgcagcg
     5161 gctggaattg gtgtatgacg gcgatcgtat cggcgttgaa ctttaataaa taacgagaat
     5221 tttatggccc tgttagtacg cctgttggca gcttctgccg tatttatttc cagct
//
LOCUS       DAOQGS010000274         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8016_length_5274_cov_5.267867, whole genome shotgun sequence.
ACCESSION   DAOQGS010000274 DAOQGS010000000
VERSION     DAOQGS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8016_length_5274_cov_5.267867"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..609
                     /gene="gspM"
                     /locus_tag="PKZ52_10875"
     CDS             133..609
                     /gene="gspM"
                     /locus_tag="PKZ52_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNG60318.1"
                     /translation="MNSIKDAFLQASPREQLLIVLGGGALVVVLLFVVFLMPMQAKAN
                     LQKRNADRVLAERQEVKELASALLGQQQNPSSAGSGSLVDMLNRTLPKYELQMEDFQP
                     NGNTDAKVRIAKADLNKVLAWLNELENVENVQIKEFSASAGPETGTAVANLRLHRE"
     gene            615..1373
                     /locus_tag="PKZ52_10880"
     CDS             615..1373
                     /locus_tag="PKZ52_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNG60319.1"
                     /translation="MKISKPWAISALVVYFLYLVISSAPATLVSSLLNKSVPNLNLSS
                     VSGTVWSGKALGANLNLEGAPLRFGPLSWHLSPLKLLMLKGCAEIKSEKLSGAVCRGL
                     TGSHQVEKLKLELPASLASNYLREANASVDGTLFLQLDNAQFSKTLQLSALKGRATWA
                     GAKVSANGMAFALGDYAAELSADQGALKAHLKDESGPIKVNLDAWLKPGAQPKVSGEI
                     HPLEHAPEAIGDALSLFAEKDDAGAYKVNYPIGG"
     gene            1471..2376
                     /locus_tag="PKZ52_10885"
     CDS             1471..2376
                     /locus_tag="PKZ52_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG60320.1"
                     /translation="MGTEVSVALWVEDAAKGREAIAAVMQDMRAVDQQFSPYITTSEL
                     AQINAQAAHKTLPISAEMAFLIDKSLYYSALTQGAFDITFASVGWFYDYRNNIRPSEK
                     QRADLMPAINYHWLDFDKTKRTLHFAHKNVRIDLGGIAKGYAVDRAIEILKGRGISHA
                     TVSAGGDSRLLGDKLGQPWRIGIKNPRPHSEKDAVITLLPLSDSAVSTSGDYERYFID
                     KQTGERVHHIINPKTGKSASEVMSVTILGPLGVDTDALSTSVFVLGVKDGLALIDRLP
                     GFDTIIIDKQGKAFYSQGLAEPTTH"
     gene            2606..3241
                     /locus_tag="PKZ52_10890"
     CDS             2606..3241
                     /locus_tag="PKZ52_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNG60321.1"
                     /translation="MSRFTLLLTLCLYTWGSWAAEAKPAAPVASPQAKPAPSQSAAKS
                     EAPPSPAASPATSPAPATGPAIGAQIEDLKKASVELNRDLMLLEEDLLFPASTQIAVY
                     VSLDVGHYFALDSVKLTIDGQMVSSYLYTPKQISALARGGVQRLYLGNLKTGSHEITA
                     FFIGKGPEDRDYKRGATYTFNKTQKPGMLEIRIKDETGAMQPTFEFKEWQL"
     gene            3229..5073
                     /locus_tag="PKZ52_10895"
     CDS             3229..5073
                     /locus_tag="PKZ52_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60322.1"
                     /translation="MAAVKLLALCLAALMPLAAFAKKPPPPSTVADLRLGVALYEYYQ
                     NKPLDALSELLVAQARGGIKGHGNHPDIMAGGFYLGYGMERTATQLFTQLLSEQQSPK
                     TRDAAWFYIARLRYDQKDWAGVEAALTHISSKPNWDLAQDILALRVNMATAQDKMAQA
                     ERLADEFERESTWRAYAYYNLGAAYGRSGNFKKAAENFDLLLHMRLERQEELSLYDKA
                     MTSAGYAYLLNKRYQEAIDQFSQVRLNSPVANRALLGYGWAAAESQNYSLALKPWTAL
                     SQRNLVDESAQEVLIALPYAYEKLGRKPDALAAYQQAEKHYLDEISQLNTSLQALLTP
                     VLDDTLNLPLDEELSWNRYAEDHNLPPQKAYFAALFAKNEFQQQVQVLKDLLQMRQVF
                     TAWQNKMQLYQDLVDERDLSRDAHMGRYQQKPYNQQVAALMAQRNALAQQLQTALANN
                     DAEILVSGREAERLRILLQAKARLKRLQAAGQASAEQVEKVRFYGGLNLMDATEQESA
                     NRYALEQNLKIVDQQLAQLSNTWQRTALEAETGVNFQSFRSRLGSARNRIDGQLKRTE
                     RAIEDNKVVLRAQIQEALEYQRARLNQYLAQTRLSIARLLDEATGEKY"
     gene            5073..>5274
                     /locus_tag="PKZ52_10900"
     CDS             5073..>5274
                     /locus_tag="PKZ52_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60323.1"
                     /translation="MHPRALVYLALLTVCGCSSLYHGFADKGPLLADLPPAKEFGEDE
                     KIQPSQLPKASLEEIERSYKAAL"
BASE COUNT         1293 a         1453 c         1410 g         1118 t
ORIGIN      
        1 ggcccgcgac atagccaccc tggaagcggt gcgcaaccaa atcgcacaaa cggggctgaa
       61 ggcggagatc aagcgggcca gtgtcgccgg tgaagtcaac caggcgctga tgcgcattac
      121 ggaaggttaa ccatgaattc aattaaagac gcttttttac aggcttcgcc ccgggagcaa
      181 ttgctgattg tgctgggcgg tggggcctta gtggtagtgt tgttatttgt tgtctttctg
      241 atgcccatgc aagccaaagc caatttgcaa aaacgcaatg cagaccgcgt attggcggag
      301 cggcaggaag tgaaagagtt ggcaagtgcg ctgctggggc agcaacaaaa ccccagctcg
      361 gccggcagtg gcagcctggt ggatatgctc aatcgcactc tgcccaagta cgaattgcag
      421 atggaagatt ttcagcccaa tggcaataca gatgcaaaag tgcgcattgc caaagcggat
      481 ctaaacaagg tgctcgcctg gttgaacgag ctggaaaatg ttgaaaacgt ccagataaaa
      541 gaattttccg cctccgcagg ccccgagaca ggcacggccg tagcgaattt gcgactgcac
      601 cgagagtaag cttcatgaag atatccaaac cctgggcgat cagcgctctc gtggtgtact
      661 ttttatacct ggtcatttcc tctgctccgg ccactttagt cagttcactt ctgaataaat
      721 ccgtgcccaa tttaaacctg tcctccgtca gcggtaccgt ctggagtggc aaggctttgg
      781 gggcaaacct aaaccttgaa ggcgcccccc tcaggttcgg ccccttaagc tggcacctaa
      841 gcccgctaaa actactgatg ttaaaaggct gcgcggagat taaaagcgaa aaactctccg
      901 gcgctgtttg ccgcggcctt accggtagcc atcaggtgga aaaactgaag ctggaattgc
      961 ccgccagcct tgcttcgaat tatttgcgcg aggccaatgc atcggtagat ggcaccttat
     1021 ttttgcagtt ggataacgcg cagtttagta aaaccctgca gctgagcgct ttaaaagggc
     1081 gcgctacttg ggcgggcgcc aaggtgtcgg ccaatggaat ggcctttgcg ctcggcgact
     1141 acgccgctga gctctcggcc gatcaaggcg cactaaaggc ccatttaaaa gatgaaagtg
     1201 gccctataaa ggtcaacctg gatgcctggt taaagccggg cgctcagccc aaagtcagcg
     1261 gtgaaattca cccgctggag cacgcgccag aagccatcgg cgatgctctg agcctgtttg
     1321 cggagaagga cgacgcaggg gcctacaagg taaattaccc cattggcggc taaggctgtg
     1381 gtgttaaagc gcgcctggct gttaagccta gtattgacgt taacggcggg gcaggtgagc
     1441 gcccagtggc tgagcgataa acaagccatt atgggcaccg aagtctccgt tgccctttgg
     1501 gttgaggatg ccgctaaggg ccgcgaggcc atagcggctg tgatgcagga tatgcgggcg
     1561 gtggatcagc aattctcgcc ctatatcacc acctcggagc tggcgcagat caacgcccag
     1621 gccgcccaca aaaccctgcc tatctcagcc gagatggcgt ttctcatcga taaatccctc
     1681 tattacagcg cgctcaccca gggggctttt gatatcacct ttgcttcggt cggctggttc
     1741 tatgattacc gcaataacat tcgccccagt gaaaaacagc gcgcagacct catgcccgcc
     1801 attaattacc actggttgga ttttgataaa accaagcgca ccctgcattt tgcccataaa
     1861 aacgtccgca ttgacctagg tggtatcgcc aagggctatg cggtggacag ggcgattgag
     1921 attctgaaag ggcggggcat tagccacgct acggtaagtg ccggtggcga tagccgtctg
     1981 ttgggggata agctcggcca gccctggcgc atcggtatta aaaacccacg cccccacagc
     2041 gagaaggacg ccgtgattac cttattgccc ctgagcgata gcgccgtgag cacatctggg
     2101 gattacgagc gctactttat cgataaacaa accggcgagc gggtgcacca cattatcaac
     2161 cccaaaaccg gtaagtccgc cagcgaggtg atgagtgtga ctattcttgg ccccctgggc
     2221 gtggatacgg atgcgctctc caccagtgtt tttgtgctgg gtgttaaaga cggcttggcg
     2281 ctgattgacc gcttgccggg ctttgacacc attattatcg acaaacaggg caaggcgttt
     2341 tacagccagg gccttgccga acctaccact cactaaacgg gtttaattcc gcccactggc
     2401 atggctaaat ttcattgccg ggtccctagc aagttcacta tagtgctgcc tctcagtctt
     2461 ttgtggctta agccacagaa cccggcgctt gctggtgttt taggcttagc ttcgggtaaa
     2521 gccgtgctaa agtaaattta ttcaaatgtt aataagccgg gcaactccca ccgaggcgct
     2581 cggcttttag tctgtggaat acactatgtc cagattcacc ctgctcctca ccctgtgttt
     2641 gtatacttgg gggagctggg cggcagaggc taagcctgcc gctcccgtcg cgtcgcccca
     2701 agccaaaccc gcccccagcc aatccgccgc caagtccgag gcgccgccaa gtccggccgc
     2761 cagcccggcc actagccctg caccggccac tggccctgca ataggcgcgc agattgaaga
     2821 cctaaaaaag gcctccgtgg agctaaaccg ggacctcatg ctactggaag aggatttgtt
     2881 gttccccgct tccactcaaa ttgccgtgta tgtatccctg gatgtgggcc attacttcgc
     2941 gctggattca gtcaaactca ccatcgatgg gcaaatggta tcgagttatc tgtatacccc
     3001 taagcaaatt agcgccctgg cgcgaggggg ggtgcagcgc ttgtatctgg gcaatttaaa
     3061 aaccggcagc cacgaaatta ctgcattttt catcggcaaa gggccggagg atcgcgacta
     3121 caagcgcggc gccacttaca ccttcaataa aactcaaaag cccggcatgt tggaaattcg
     3181 catcaaggat gaaaccggtg caatgcaacc cacttttgaa tttaaagaat ggcagctgta
     3241 aaactcctcg cattgtgttt ggcggcgctt atgccgctgg cggcctttgc caagaagcca
     3301 ccaccaccga gcaccgtagc cgacttgcgc ctgggggtag ccttgtatga gtactaccaa
     3361 aacaaacccc tggatgcctt aagcgagctg ctggtggccc aagcgcgcgg cggcatcaaa
     3421 ggccacggca accacccgga catcatggcc ggtggctttt atctggggta cggcatggag
     3481 cgcaccgcca cccaattatt tacccagctg ctgagcgagc agcagagccc caaaacccgc
     3541 gacgccgcct ggttttatat cgcccgttta cgctacgacc aaaaagactg ggctggggtg
     3601 gaagcagcgc tcacccacat cagctccaaa cccaactggg atctagccca ggatattctc
     3661 gccctgcggg tgaacatggc caccgcccag gacaaaatgg cccaggccga gcgcctggcg
     3721 gatgagttcg agcgggaatc cacctggcgg gcttacgcct attacaacct cggggccgct
     3781 tatggccgca gtggcaattt taaaaaggcg gcggaaaatt tcgatttact cctgcatatg
     3841 cgccttgagc ggcaggaaga actaagcctg tacgataaag ccatgaccag cgcaggctat
     3901 gcctatttat taaataagcg ctaccaggaa gccatcgacc aattctccca ggtgcgcctc
     3961 aatagcccgg tagccaaccg cgccctgtta ggttacggct gggcggcggc ggaatcgcaa
     4021 aactacagct tggccttaaa accctggacg gccctatccc agcgcaattt ggtggatgaa
     4081 agcgcccagg aggttttaat tgcgctgccc tatgcctatg aaaaactcgg ccgcaagccg
     4141 gatgcactag ccgcctatca acaggcggaa aaacattatt tagatgaaat aagccaatta
     4201 aatacctccc tgcaagcgct gcttaccccg gtattagacg acacattaaa tttgccgctg
     4261 gatgaagaat taagctggaa tcgatacgcg gaagatcaca atttgccgcc gcaaaaagcc
     4321 tattttgccg ccctatttgc caaaaatgaa tttcaacagc aggtgcaagt gttgaaggat
     4381 ttgttgcaaa tgcgccaggt atttacggcc tggcaaaaca aaatgcagct ctaccaggat
     4441 ttagtggatg agcgggatct aagccgcgac gcccatatgg gccgttacca acaaaaaccc
     4501 tataaccaac aagtggcggc cttaatggcc cagcgcaacg ctctcgccca gcagttacaa
     4561 accgctctgg ccaataacga cgcggaaatt ttagttagtg ggcgcgaggc tgagcgtttg
     4621 cgtatcctgc tgcaggcaaa agcacgcctt aagcgtttgc aagcggccgg ccaggcaagc
     4681 gccgaacaag tggaaaaagt gcgcttttac gggggcctta atttaatgga cgccaccgaa
     4741 caggaatccg ccaaccgcta tgctctggag caaaacctta aaattgtcga tcaacaattg
     4801 gcgcagttaa gtaacacctg gcagcgcacc gcccttgaag ctgaaaccgg cgttaatttt
     4861 caatcctttc gcagccgttt aggcagtgcc cgcaatcgca ttgatggcca attaaaacgc
     4921 accgagcggg ccattgaaga caacaaggtt gtgctgcgcg ctcagatcca agaggccttg
     4981 gagtatcaac gggcgcgttt aaatcagtat ttggcgcaaa cccgtttatc tattgcccgc
     5041 ctgctggatg aagccacggg ggagaaatat taatgcaccc cagagcactc gtttatttgg
     5101 cgcttttaac cgtttgcggc tgcagcagtc tctaccatgg ctttgccgat aaaggcccat
     5161 tattggcgga tttaccgccc gctaaagaat ttggcgagga tgaaaaaatc cagcccagcc
     5221 aattgcccaa agcctcttta gaggaaattg aacgcagtta taaagccgcc ttag
//
LOCUS       DAOQGS010000275         5269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8025_length_5269_cov_4.982739, whole genome shotgun sequence.
ACCESSION   DAOQGS010000275 DAOQGS010000000
VERSION     DAOQGS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8025_length_5269_cov_4.982739"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2240
                     /locus_tag="PKZ52_10905"
     CDS             <1..2240
                     /locus_tag="PKZ52_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNG60324.1"
                     /translation="VLGTAVMIIGGNSRSVSQAVAAQLNKINKTLPPGVQAVPVYDRT
                     VLVNKTIATVQKNLLEGAILVAAVLLVMLGNWRAALLTAMVIPLSMLILMTGMVHTKV
                     SANLMSLGALDFGLIVDGAVIIVENCLLQLAQRQDAVKRLLTLDERLHVVFNATQEVF
                     MPSLLSVLVVILVNVPILALTGVEGKMFTPMAAAVIMALIAALILSLTFVPAAVALFL
                     TGPIKEQDNYGVQRIKQLYSAALKHALHLRVSVLILACVFIVVCGFIAGRLGHEFMPN
                     LDEGDVAVQALRIPGTSLSQSLDMQFQVEKAVLQVPEVKTFFSRVGTAEVASDPMGPN
                     IADGYVMLKDNHQWPQPKRTKAQVVADIEEKLSQVPGNAYEISQPIQLRFNELISGVR
                     SDLGIKLYGDDLEQLLAAGNQIAALLTNIPGAQGVKVEQVDGLPILSVDAKPQALLRY
                     GLNISDVQHTLAIATGGETAGQVFVGDQRFAIVVRLPEIVRTDLTALARLPILLADGG
                     YVPLQEVAQLNITPGANQISRENGKRRLVVSADVAGRDLGGFVREVQTQIDQRIKLPP
                     GYWLAYGGTFEQLQSASERLSILVPLTLVAIFVLLMVTFASAKDAALVFSGVPLALTG
                     GVLALWLSGIALSITAAVGFITLSGVAVLTGVMMVSAFRAELNSGLAIEAAIMSGALK
                     RLRPVLMVALVAALGFLPMALNTGTGAEVQRPLAVVVIGGIISSTLLSLLVLPGLFRL
                     AYNKD"
     gene            complement(2603..3178)
                     /locus_tag="PKZ52_10910"
     CDS             complement(2603..3178)
                     /locus_tag="PKZ52_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNG60325.1"
                     /translation="MQSISTIDLLLTRRSVSPKDMQGEGPSAEQIDTLLRAAHRVPDH
                     GKLGPWRFVVFTDEARRDFGRRLGEIYQADFPSVTADLKTVQEHLFLRAPVIIAVIST
                     AGPHVKIPEWEQVLSAGAACQNILVASHALGFKACWLTEWYSYHPQVKALLGLEPSHN
                     IAGFIYIGNYPGTPEDRVRPPLADRVSYWPI"
     gene            3584..4375
                     /locus_tag="PKZ52_10915"
     CDS             3584..4375
                     /locus_tag="PKZ52_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNG60326.1"
                     /translation="MQYRFNLLKLLPLLAAVCSPLASATVVSITIDGGDAIFLAGRTD
                     LTIPPASDAWDGPGDHTIRHSGPTPEEIVETLPPIVSVAAGDVIRALDPAVGGISFYN
                     GLGAPFYGPSGNGLSGSNLSALGGISGYIGPQGALAGVFLTDAIPNGIAPITLNFNPD
                     GLGAEFSVLNPAVGQVFYIGDGKNSAGEFQTFIAPTGATRLALGIPDGFGFNGAPGAY
                     DDNDGSYSIRLGINEIPTPVPEPASISMMLAGLGLCALVRRRIKK"
     gene            4829..>5269
                     /locus_tag="PKZ52_10920"
     CDS             4829..>5269
                     /locus_tag="PKZ52_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNG60327.1"
                     /translation="MTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFLLRIEDT
                     DQVRSTPQSEAAILASLRWLGLEWDEGPDVGGPHGPYRQSERAAIYAGYAEQLLAQGH
                     AFKCYRTAAELDEIRAARKEAGISSALKPSDLILPADEIARREAA"
BASE COUNT         1162 a         1215 c         1468 g         1424 t
ORIGIN      
        1 ccgtattggg caccgccgtt atgatcattg gcggcaatag ccgcagcgta tcccaagcgg
       61 tggcggcgca attaaataaa ataaataaaa ccctgccgcc gggggtgcag gcggtgccgg
      121 tgtacgatcg cactgtgctg gtgaataaaa ccatcgccac cgtgcaaaaa aatttactgg
      181 agggcgcaat tttagtagcc gcagtattgc tagttatgct cggtaattgg cgtgccgccc
      241 tgcttaccgc catggtgatt ccactatcta tgctgatatt aatgaccggt atggtgcaca
      301 ccaaagtcag tgccaattta atgagtttgg gcgcgctgga ttttggtttg attgtggatg
      361 gcgcagtgat tattgtggag aactgtttat tgcaattggc ccagcggcaa gacgcagtaa
      421 agcgcttgtt aaccctggat gagcgtttgc acgtggtatt caatgcaacc caagaggtat
      481 ttatgccgag cctcttgagt gtattggtgg tgattttagt taatgtgccg atccttgcgt
      541 taaccggcgt ggagggcaaa atgtttacgc ctatggcggc tgcggtcatt atggctttaa
      601 ttgccgcttt aattttatcc ctcacctttg tgcctgcagc ggtggcgtta tttttaactg
      661 ggccgataaa agagcaagat aattatgggg tgcagcgtat taaacagctg tattcggctg
      721 cactaaaaca cgcgctacac ctgcgtgtga gcgtgctcat acttgcctgc gtatttattg
      781 ttgtgtgtgg ttttattgcc gggcgtttag gccacgaatt tatgcctaat ttggatgaag
      841 gcgatgtggc ggtgcaggcc ttgcgtattc ctggcaccag tttaagccaa tcattagaca
      901 tgcagtttca agtggaaaaa gccgtgttgc aggtgccgga ggtgaaaacc tttttctccc
      961 gcgtgggcac ggcagaggtg gccagtgacc ccatggggcc gaatatcgcc gatggttatg
     1021 tgatgcttaa agataatcat cagtggccgc agccaaagcg cactaaggcg caggttgtag
     1081 ccgatataga agaaaaatta tcccaagtgc ccggcaatgc ctatgaaatt agccagccta
     1141 ttcaattgcg ctttaatgaa ttaatttccg gtgtgcgctc agacctcggc attaaactct
     1201 acggcgatga tttagagcaa ttactggcag cgggtaatca aattgcagca ttgttaacga
     1261 atattcccgg cgcccaaggc gtaaaagtgg agcaggtgga tgggctgccg attttatcgg
     1321 tggatgccaa gccccaagcg ctgcttaggt acggcttaaa tatcagcgat gtgcaacata
     1381 cgctggccat cgccacgggt ggtgaaactg ctgggcaagt atttgtgggg gaccagcgct
     1441 ttgctattgt ggtgcgcttg ccggaaattg tgcgcacgga tttaaccgct ttggcgcgcc
     1501 tgccgatttt attggccgat ggcggttatg tgccattaca agaagtggcg caattgaaca
     1561 tcacaccggg tgccaatcaa attagccggg aaaatggcaa gcgccgttta gtggtgagcg
     1621 ccgatgtggc gggccgcgat ttaggtggtt ttgtgcgcga agtgcaaacg caaatagacc
     1681 agcgcattaa attaccgccg ggatattggc tggcctatgg tggcacattt gaacaattac
     1741 aatcggctag cgagcgctta agtattttgg tgcctttaac cctggtggcg atttttgttt
     1801 tattaatggt gacctttgcc tccgctaaag atgccgcttt ggtatttagc ggtgtgccgc
     1861 tggccctcac cggcggtgtt ctcgctctgt ggttgagcgg cattgcgcta tcaattaccg
     1921 ctgcagtggg ttttattacg ctttccggtg tggcggtgct caccggtgtc atgatggtgt
     1981 cggcatttcg cgccgaatta aacagtggtt tagcgattga agcggccatt atgagcggtg
     2041 cccttaagcg actgcggccc gtgttaatgg tggctttggt ggcggccctg gggtttttac
     2101 ctatggccct taataccggc acgggcgcag aagtgcaacg gcctttagcg gtggtggtga
     2161 tcggcggcat tatttcttcc accttgctga gtttgttagt tttaccgggg ttatttcgct
     2221 tggcctataa caaagattag tcctgcggcc tacaccagcg ttgcgcggtg tgggccgtct
     2281 ctcttaccta taattgcact aaaaaattcg ccatagttcg gtaagtaagg cgcagtttgt
     2341 ttttgcggca gttttgtatg ctgagcatta atcctgccga tagattttaa aagttaaata
     2401 aaaaatgtaa ctattccgca ccattttgtt gtggctgggc gtggtttttg tgccgaatag
     2461 aaatcaccta tattcatcgc tgcgtggggg tgtattgatg cctgaaggtt tctatctagt
     2521 tatccagtca gtgcgagaat cacgtccagt tacgttaaaa aggtgctgaa ttattttaat
     2581 aaagatagac aatctgtgtt atttaaatcg gccagtagga aacgcgatca gccaagggcg
     2641 ggcgcacgcg atcttccggt gtgcctgggt aattgccgat ataaataaaa cccgcaatat
     2701 tgtgcgaagg ttcaagcccc aagagcgctt taacctgggg gtgataggaa taccactcag
     2761 ttaaccagca ggctttaaaa cccagggcgt ggctggctac taaaatattt tggcaggcgg
     2821 cgcccgccga gagcacttgt tcccattcgg gaattttaac gtgggggcct gcagtggaaa
     2881 taacggcaat aatgaccggt gcgcgcaaaa ataagtgttc ttggacggtt tttaaatcgg
     2941 cggtgacaga cggaaaatcc gcctggtaaa tttcccctaa acggcggcca aaatcacgcc
     3001 gcgcctcgtc ggtaaatacc acaaaccgcc atggccctaa cttgccgtgg tccggcaccc
     3061 ggtgggcggc gcgcaaaagg gtgtcaatct gctccgcgct cggcccttca ccttgcatgt
     3121 cttttggtga taccgaacgg cgcgttagca ataaatcaat agtcgatatg gactgcataa
     3181 aacctccaga aaatccagat tttagccgct ttgctggcaa cagggaagaa aagccttgtg
     3241 cggctgtgca accgcttgct gggcaatgta tggcaccgat tgttgataaa aaacggtggc
     3301 gcaggatgaa gttgccttga tagttaaact cactttttgc agtagttgtc gttttatagc
     3361 ttgtgaaata ttcggcagat gtaccggtaa ttattgctgt cgggcaggtg attatatgtg
     3421 atagggttta tcagtgcggc aggagtgatt aacatgcgct acagcggcac ggcagccatt
     3481 aacaactgtg aacctgggtg atgttatcgg tgttttttta tggggtagct gcgatagtcg
     3541 gcgatgatca aaaatagtta acaattatgt ggagtgagaa tttatgcaat atcgattcaa
     3601 cctattaaaa ttattgccgc tgctggcggc ggtctgttca cctttggcct cggcaacggt
     3661 ggtgagtatt accattgatg gtggcgatgc catattctta gccgggcgca cagatttaac
     3721 gattccgccc gccagcgatg cgtgggatgg gccgggcgat cacaccatcc gccacagcgg
     3781 ccccacaccg gaagaaattg tggaaacctt gccgccgatt gtatcggtgg ccgcaggtga
     3841 tgttatccgt gctttggacc ctgctgtggg cggtattagt ttttacaacg gcctgggcgc
     3901 gcctttttat gggccgtctg gcaatggttt atcaggcagt aatttatccg cattgggcgg
     3961 catcagcggc tatattggcc cccagggtgc attggccggt gtgtttttaa ccgatgctat
     4021 tcccaatggt atagcgccca taacacttaa ttttaatccc gatggtttag gtgcggaatt
     4081 ttcggtgctc aacccggcgg tggggcaggt gttttatatc ggcgatggca aaaattcagc
     4141 gggtgaattt caaaccttta ttgcccccac gggagccact cgcctcgcct tgggaattcc
     4201 cgatgggttt ggttttaacg gtgcacccgg tgcctatgac gataatgatg gcagttattc
     4261 tattcgttta ggtattaatg aaattcccac gccagtgcct gagcctgcca gtattagcat
     4321 gatgttggcg gggcttgggt tgtgtgcgtt agtgcggcgg cgcataaaga aataatcgcc
     4381 aagtaaaata cttctgtaca taattattta ctgagttatc acctactgcc gcttttaagc
     4441 ggcagttttt ttacgcgtat ggtttagcgt gtatatccag aaacattatc gcccggttta
     4501 aattctccgc gcgcatctaa aatatttcgg cttggggtgc attattaacc tggcggcaaa
     4561 atagatcgtc ctgatcaatt ttgctttcgc tgcgctagac acgtgcgaaa atgcgcggat
     4621 tttcgtgttt tagcgtagct cgcggcggtt tgcaccgctg gggcggcatt cttgccgcca
     4681 ttattaaccc ggcactaaat gccgccgggt taatagctat cgcattcttt tgaattatct
     4741 cggccctacg tggccggggc agcaaggcgc tttttgtccg ccaacatggc gtataatcgc
     4801 cgcctttttc accttgagaa gattgtgtat gacggtgcgc acccgcatag ctccctcgcc
     4861 cacgggcgac ccccacgtag gcacggccta tattgccttg tttaatttgt gttttgcccg
     4921 ccagcacggc ggccagtttt tgctgcgcat tgaggatacc gaccaggtgc gctccacccc
     4981 gcaatcggag gcggccattc tggcttcctt gcgttggttg gggttggagt gggatgaggg
     5041 cccagatgtg ggcggccccc acggccccta tcggcagagc gagcgtgcgg ccatttatgc
     5101 cggttacgcc gagcagttat tggcccaggg ccatgccttc aaatgctacc gcaccgccgc
     5161 tgagttggat gaaattcgcg cggcgcgcaa agaggcgggt atctccagcg ctttaaaacc
     5221 ctcggattta atcttacctg ccgacgaaat tgcccgtcgg gaagccgct
//
LOCUS       DAOQGS010000276         5260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8048_length_5260_cov_4.029587, whole genome shotgun sequence.
ACCESSION   DAOQGS010000276 DAOQGS010000000
VERSION     DAOQGS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_5260_cov_4.029587"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKZ52_10925"
     CDS             <1..278
                     /locus_tag="PKZ52_10925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820100.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNG60328.1"
                     /translation="HKKEAACGCAGAYTAYSALELYAEAFERAGKLAALEAFASFNGP
                     DFYGLPRNKGQVTLVKQDWQLPAQLPFGSEQVIPLMAGETLHWRLQR"
     gene            293..940
                     /gene="rnt"
                     /locus_tag="PKZ52_10930"
     CDS             293..940
                     /gene="rnt"
                     /locus_tag="PKZ52_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HNG60329.1"
                     /translation="MTADTSAKTLFAERFRGFMPVIVDVETGGFNAQTDALLEIAAVT
                     LRMDEQGFLAVDETHSFHVDPFEGANIEQSALDFTGIDLACETREAVPEQLAMEEIFA
                     AVRRKVKEYGCNRAVIVGHNAHFDLGFVKAAANRCDLAKRNPFHLFSCFDTATLAGLA
                     YGHTVLARACQLAGIEFSNKEAHSAAYDAEKTAELFCGVINKWQILGGWPLGAPE"
     gene            1571..3271
                     /locus_tag="PKZ52_10935"
     CDS             1571..3271
                     /locus_tag="PKZ52_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNG60330.1"
                     /translation="MTNNQLNLFKLSGSCVLALALAACTGTPSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSTGGGNSSSSSSSSSSTGGSNGGSTTSSSSSSSSSSSSSGTIVSGP
                     GRYRINAQGRVTENGTVFPMKCGAWFGLEGQHEPSDAKANPSGAPLELYIGNMWWDNN
                     SKRTIQQTMTEIKSLGINMIRLPVTPQTLEPNNEQGMTRVWNGSTQNPNGRLKNKADA
                     YPYANARTAMIEFIKLAQTNGLKVMPDIHSCSNYVGWRKDVFEANPPYVDKDRVDYDY
                     KREDHSCGGGATNDKPYNKTKWLENLKDLATLAKTYNNIIGIDIFNEPWGYTWDQWAT
                     LAEEAYQTINSVNQDVLIVVEGIGSSLKDGTKVPHGDLATNPNWGENAYGFQTRPLNI
                     PKDRLVISPHVYGPSVYQQGHFFSSSCGTNEEGNTYNGDEAAKNNCQFDIAGNKTRIK
                     AGWQEHFGYLRDQNYAILIGEYGGNMDWPQKGTRAAEKQLWSYVTNTPDKAWQETLGE
                     YMVEKKIEGCYWSINPESGDTGGIYIHKYDPISNTEGWSQWGEMDSRKTALLKKIWAQ
                     "
     gene            3374..4222
                     /locus_tag="PKZ52_10940"
     CDS             3374..4222
                     /locus_tag="PKZ52_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60331.1"
                     /translation="MSEESAVQKSKKTLWVLVSLGVLVVVVVLGALQHIKPIFGLSSK
                     DAEALTQKLNNLKVGVDISFFTQQLGEPKIKENKTIKLVSYKIIRHTDPKKEVREVVY
                     NEYYYRSPYFYVQAVANEAGVVQLYSITARNKQFQPKIKTVLNESVQLGASVYQTLPP
                     KLPYKVAGRLAEGPANAAYYEIFVLESALPKLEIYSTSPFGIIKDTVPLDEKQEGFLV
                     SKFFEGTTFPITPQHDAFRKTTVINTYTAVTAEFEGIDTSPDGMNYGETKFTFGPRED
                     QIQKLQ"
     gene            4532..>5260
                     /locus_tag="PKZ52_10945"
     CDS             4532..>5260
                     /locus_tag="PKZ52_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNG60332.1"
                     /translation="MSALSAPAADETLLLKDFTEKAYLDYSMYVILDRALPHIGDGLK
                     PVQRRIIYAMSELGLKANAKFKKSARTVGDVIGKFHPHGDSAAYEAMVLMAQSFSFRY
                     PLVDGQGNWGSPDDPKSFAAMRYTESRLTAFSELLLSELGQGTVTWQPNFDATLEEPT
                     VLPSRVPALLLNGTTGIAVGMATDIPPHNLREVVSACVHLLDNPNASTADLCQHILGP
                     DMPTRAEIITPRADIIKMYETGRGS"
BASE COUNT         1346 a         1339 c         1311 g         1264 t
ORIGIN      
        1 gccataaaaa agaagccgcc tgcggctgtg ccggtgccta caccgcctat agcgccctgg
       61 agctgtacgc cgaagccttt gagcgggctg gtaagcttgc cgcactggag gcctttgcca
      121 gctttaacgg gccggatttt tacggcctgc cgcgcaataa gggccaggtc accctggtga
      181 agcaggattg gcagctgcct gcgcaactgc ccttcggcag tgagcaggta attcccctga
      241 tggccggtga aaccctccac tggcgcctgc agcgatgaac aaggagcccg ccgtgacagc
      301 cgatacctcc gctaaaaccc tatttgccga gcgctttcgc ggctttatgc cggtgattgt
      361 ggatgtggag accggcggct ttaacgctca gacggacgcg ctgctggaga ttgcagcggt
      421 caccctgcgc atggacgagc agggcttttt agcggtggat gaaacccact ccttccatgt
      481 ggatcccttt gaaggtgcca atattgagca atcggcactg gattttaccg gcatcgacct
      541 cgcctgcgag acccgcgagg cggtgcctga gcagctggcc atggaggaaa tttttgccgc
      601 tgtgcgccgc aaggtgaagg aatacggctg taaccgcgcc gtgattgtgg gccacaacgc
      661 ccattttgac ttgggttttg tgaaggccgc agccaaccgc tgcgatttag ccaagcgcaa
      721 cccatttcat ctatttagct gctttgatac ggcaaccctc gcgggcctgg cctacggcca
      781 caccgtgctc gcccgcgcct gccagttggc gggtattgag ttctccaata aagaggccca
      841 cagcgcggcc tatgacgctg agaaaaccgc cgaattattc tgcggtgtga tcaataaatg
      901 gcagatactc ggcggctggc ccctgggcgc cccggaataa gcttcccacc tgcaccacaa
      961 aaagcctttg tttataggat aaatgctcgt tttgcgccgc tttgacgccg tcgtgaaaat
     1021 aacttgtatc cagttcacag tacgaatgac catcaggtta tgcgcaaaaa gacaaactgc
     1081 gaaggccatc atacttacat gatttgacgg atgacgcact ggcaattgtt aggcttaata
     1141 ggtactaaca gggttggaaa gtaccgcctg aaggtcgctt atttgaagtg ttttcactct
     1201 atgtgaagca gatttagata agtagcagcg aggcggtagc ggcaaagcaa taacaacagt
     1261 cgctaccagg tgctttaaaa caggtgtttt tgccgctacc cagcgtgcaa agacatcggc
     1321 ttcctctgga aatggatgtt ttaacctttg tacaccctgc ggtggtgctg tattaggtgt
     1381 tctgtagtga gccgctcttt aattaagagt agctgtgttg cgtgaggtgt gatggtttag
     1441 ctcgggtttt gttaggtagt tcgcactaat taaccgaata acatcaagcc gcacaataca
     1501 gactgccggt tgccgccgtg cgagatgaat aaaaacataa ttatttttaa ctagccgatg
     1561 tgggattagt atgaccaaca atcaattaaa tttatttaag ctgtccggct cctgtgtgtt
     1621 agcactggcc ctggcggctt gtactggaac gccttccagc agttcgtctt ctagcagcag
     1681 ctcctcttct tcaagcagca gctcttcttc aagcagctct tcctcttctt ctagcagttc
     1741 tactggtggt ggcaatagca gctcaagctc ttctagctca agctcaaccg gtggcagcaa
     1801 cggcggatca accacctcct cttcgtcttc ttcctcatct tcgtcctcct caagcggaac
     1861 catcgtgagt ggaccaggtc gctaccgcat taacgctcaa ggccgcgtga ctgaaaacgg
     1921 cactgtattc ccaatgaagt gtggcgcgtg gttcggtttg gaaggtcaac acgagccttc
     1981 tgacgccaag gctaacccaa gcggtgcgcc tttagagcta tacataggca acatgtggtg
     2041 ggataacaac agcaagcgca ccattcaaca aaccatgacc gagattaagt cactcggtat
     2101 caacatgatt cgtttgcccg taacaccaca aactctggag ccaaacaacg aacagggtat
     2161 gacccgcgtt tggaacggca gcacacaaaa ccccaatggt cgcctgaaaa acaaagccga
     2221 tgcatacccc tatgcaaacg ctcgcacggc aatgatcgaa ttcattaagc ttgcccaaac
     2281 taacggctta aaagtaatgc ctgatatcca ctcttgctcc aactatgtgg gctggcgtaa
     2341 agatgtgttc gaagctaacc ctccttatgt tgataaagat cgcgttgatt acgactacaa
     2401 gcgtgaagat cactcttgtg gcggcggtgc gactaacgat aagccataca acaagacaaa
     2461 gtggttagaa aacctgaaag acctggctac tcttgccaaa acctacaaca acattatcgg
     2521 tatcgatatc ttcaacgagc catggggtta cacctgggat cagtgggcga ctttagctga
     2581 agaagcctat caaactatta attccgtcaa ccaagacgtg ttaattgttg tagaaggtat
     2641 aggttcaagc ttgaaagacg gtactaaagt tcctcacggt gacctagcca ccaacccgaa
     2701 ctggggtgag aacgcctatg gtttccaaac ccgtccgctg aacattccta aagaccgttt
     2761 ggttatctct ccgcacgtat acggaccttc cgtgtaccag caagggcact tcttctcctc
     2821 tagctgtggt accaatgaag aaggcaatac ctacaacggt gatgaagcag ccaagaacaa
     2881 ttgtcaattt gatatagctg gtaacaagac gcgtatcaaa gctggttggc aggagcactt
     2941 cggttacctg cgtgatcaaa actacgctat cttgatcggt gaatacggtg gtaacatgga
     3001 ttggccgcaa aaaggtacgc gcgcagctga aaaacaactg tggagctacg taaccaacac
     3061 cccggataaa gcctggcagg aaactctagg tgagtacatg gttgagaaga aaatcgaagg
     3121 ttgttactgg tcgatcaacc ctgaatcagg tgacaccggc ggtatctaca tccacaaata
     3181 tgatccgatc agcaacaccg aaggttggag tcaatggggt gagatggatt cacgtaaaac
     3241 cgctctgttg aagaaaatct gggcgcaata attcccgatt ttcgccggta aaaataaacc
     3301 cggagctggc agcagctccg ggttttttgt tgtctgttga tcctcttgtt gtttttgggt
     3361 atgagtaagg tctatgagtg aagaaagcgc agttcaaaaa tccaaaaaaa cgctttgggt
     3421 tttggtgagt ttaggtgttt tagtagtcgt agtcgtgctg ggagcactgc agcacataaa
     3481 gcccattttt ggtttgtcca gcaaagatgc cgaggcctta acgcaaaagc tcaacaatct
     3541 gaaagtgggc gttgatatca gtttttttac gcagcaactg ggtgagccaa aaattaagga
     3601 aaataaaacc ataaagctgg tgagttataa aataattcgt cacaccgatc ccaaaaaaga
     3661 agttcgggag gtggtgtaca acgagtacta ctacagaagc ccgtattttt atgtgcaggc
     3721 tgttgccaat gaggcgggag ttgtgcagct ctattcaatt accgcccgca ataagcaatt
     3781 tcaacctaaa attaaaaccg tcttaaacga atcagtgcaa ttgggcgcca gtgtgtatca
     3841 aacgctgccg cctaaactgc cgtataaagt ggctggccgt ttggccgagg gccctgcgaa
     3901 tgccgcctat tacgaaattt ttgtgctcga atccgcgctg ccaaaattgg aaatatattc
     3961 aacaagccct tttgggatca taaaagacac agtgcctttg gatgaaaaac aagaagggtt
     4021 tttggtgagc aaatttttcg agggaacaac atttccgatt accccgcagc acgacgcatt
     4081 taggaaaacc actgtcatca atacctatac tgctgtaacg gctgagtttg aaggcataga
     4141 tacttctccc gatggaatga attatggaga gacgaaattt acatttggcc ctagggaaga
     4201 ccaaatacaa aaattgcagt aaattcattt cggtggctgt aaagctcccg cttcattaac
     4261 caaacatctg gttgcataat ttaggtgttt ggttagctca aaatcccacc ctaattccta
     4321 actcgcgtgc agcttgtact tcaaaggttg cggcacccgg gcggcgctta gggttctgca
     4381 taaatctatt tgtcctctgt gcgcttcttg tcggcccaaa cccgcaccag cttgccaaac
     4441 ctactgcctg agcactcgcc tacaaacacg ttcacctaag gggcagcgca tggtttaata
     4501 cgcgcacttt tcactggtac ttgatacgac tatgagcgca ctttccgccc ctgcggccga
     4561 tgaaacactg ctattaaaag actttaccga gaaggcttat ctggattatt ccatgtatgt
     4621 catcctcgat cgcgcgttgc cccacatagg tgatggcctc aagccggtac agcggcgcat
     4681 tatttacgcc atgagcgagc tgggcttgaa ggcgaacgcc aaattcaaaa aatccgctcg
     4741 caccgtgggg gacgtgatcg gtaagttcca cccccacggc gattccgccg cctatgaagc
     4801 catggtgctg atggcacaat ccttttcctt tcgctacccg ctggtggatg gccagggcaa
     4861 ctggggttcg ccggacgacc ctaaatcctt cgccgccatg cgctacaccg aatcgcgttt
     4921 aaccgcgttt tccgagttgt tattaagtga attgggccag ggcactgtca cctggcagcc
     4981 caacttcgat gccactttgg aggagcccac cgtattgccc agccgagtgc ctgccctact
     5041 gttaaatggc accaccggaa ttgccgtggg tatggctacc gatattccgc cccacaatct
     5101 gcgtgaagtg gtcagcgcct gtgtgcatct gttggataac cccaatgcga gcacagccga
     5161 tttgtgccag cacattttgg ggccggatat gcccacccgg gcggaaatca tcacccctcg
     5221 cgccgatatt atcaaaatgt atgaaactgg ccgcggcagt
//
LOCUS       DAOQGS010000277         5236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8111_length_5236_cov_8.049604, whole genome shotgun sequence.
ACCESSION   DAOQGS010000277 DAOQGS010000000
VERSION     DAOQGS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8111_length_5236_cov_8.049604"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PKZ52_10950"
     CDS             complement(<1..647)
                     /locus_tag="PKZ52_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60333.1"
                     /translation="MPNPFEKFGYAEDLPAPEQNATTENPFARFGFEEDSAPAPEARP
                     ERTLLGTAKDVGITALKGAINLPQAFVGLGDIATGGRVGKYLEEVGYRPEDAKQMLDE
                     GLSPAQQYANEQVHNAKGFGGTIETLYHNPSAAVTTVGESLPQMLGGAGIARGIMTAA
                     PRMAPLVAGAVGEGAIAAGSNAEQIRSKTEDGLLTPEQQLAAAASGVGTAALGVAG"
     gene            complement(653..1822)
                     /locus_tag="PKZ52_10955"
     CDS             complement(653..1822)
                     /locus_tag="PKZ52_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60334.1"
                     /translation="MENAQQQSQINAIRVRDLNDERVAKMNKALAWSMHSLKDAPEEY
                     RSFVATHPNLDAGRILSPEYGQAIETLKGAAEGRVDYRSPEVAAAFDVLNPEIGLGAT
                     QGRKVSTSRLYPGRTPGTLMVGLKVDGDPEERPLTERRSSDPDDPVKEVKLEDLFGRL
                     KVAEQYRGFLSDPENQKWFIQQTIGEELKKSADQETIKNLQVKKLTKEINEGDPVTYR
                     ADGEGNLMQLPSRSGGAVATPVLDSKGNPVKAPQKSGAGASSKSSLEKEVEYLKTLGM
                     TPDEALAYRKQRTGNDAKDTELMAMAISRQSQIPMEDYADDKNPEQKLPGAITQAINA
                     RQKIKALTQAKSDQPAPTPTGQPRKAPQAAIDELRKNPSLKDKFQEFYGYLPDGM"
     gene            complement(2016..2255)
                     /locus_tag="PKZ52_10960"
     CDS             complement(2016..2255)
                     /locus_tag="PKZ52_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60335.1"
                     /translation="MQMSSSVKNSALGGLRDAAMLEERRNQANRALKEQKSQTEKMSM
                     GTGAATGAMYGAMAGGPVGALIGGGIGLLGGYLLS"
     gene            complement(2270..2728)
                     /locus_tag="PKZ52_10965"
     CDS             complement(2270..2728)
                     /locus_tag="PKZ52_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60336.1"
                     /translation="MAGLSQLSTAAQGKAPVADWIKNVSGLGGRSASDTFAQITRDQW
                     ADYKNTFAPYEDQLIALSTGNADNLQSEQRAAGAVENSFNSSLGTLARDRSRLGVSIG
                     SDQAADEKRLGATLKTASLVGATNKARLHAQDRDKAVLTGGLSSLRGAQQ"
     gene            complement(2738..3451)
                     /locus_tag="PKZ52_10970"
     CDS             complement(2738..3451)
                     /locus_tag="PKZ52_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60337.1"
                     /translation="MIDLLDGCLIEPPRFQPMARFRKFGAQRVFYKGSAPQQKETEQE
                     KALAQVANEQWARYKEVGIPAREAFFKAVNDSGTEPANSFARGTAASATKAKYGGLNM
                     VKPGVGASSPANTSGGFTLAMARAKDTAGNTQGASLSQEDMHVGGLQDIVSIGRGEAT
                     QASAGLSDLAIKANQKATQDVTNQFNNRAANMSAAGTLVGAGAQLYGGGLNDKKAWQK
                     KTNAQYGFGDSLGSSDPFA"
     gene            complement(4100..>5236)
                     /locus_tag="PKZ52_10975"
     CDS             complement(4100..>5236)
                     /locus_tag="PKZ52_10975"
                     /inference="COORDINATES: protein motif:HMM:NF020885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HNG60338.1"
                     /translation="AASAVDYTAITARLNNVGGLTIEQKFDAQASSITGLYGQYTLKI
                     DVNGRVSGFGLASTNTASTFIINADKFAIGKPGATGSDIYPFIVDTTTGQVVMDGAFI
                     KNATINDLQVGGVTAGKITGLKASFITVDAGQANIDEGSVSQGIQSSNYSAGVSGWKI
                     HNNGNAEFNDLVARGTLSSSNYNPGNAGWQIQKSGAAEFNGVVITRANVVADSGSAGN
                     YYPPAGWHFEKVYVSAPSAGNRWFYVRGNDLIYDGTYTEQSVYAAAHDGKNGGVTFAV
                     DTGYITTDDITDNLSASFVGRAQVFTASYSYSGGAFSGTAFYLDAECSVGFMRTWAGP
                     LGADPDQSQKRIILYITVRLNPDVHPQVTNIRANQISWALHRIT"
BASE COUNT         1072 a         1523 c         1334 g         1307 t
ORIGIN      
        1 cccgccacac ccagtgcagc ggttccaaca ccagatgcgg cggccgctag ctgctgttcc
       61 ggggtgagca ggccatcttc ggttttgctt ctaatttgct cagcattcga tccggccgca
      121 atggcgccct cacctacagc gccagccaca agcggtgcca tcctgggggc ggcagtcatt
      181 atcccgcgag caataccggc tccgccaagc atttgcggga gagattcgcc aacggttgta
      241 actgccgcac ttggattatg atacaaggtc tcaatggttc ccccgaagcc cttggcgtta
      301 tgcacctgct cgttggcata ttgctgcgcg ggtgatagcc cctcgtccag catttgcttt
      361 gcatcttccg ggcgatagcc aacttcttcg aggtacttgc cgactctgcc gccggtggca
      421 atatcgccaa gaccaacgaa tgcttgcggc agattgatcg cccccttaag tgccgtaatg
      481 ccgacatctt tggcggtccc aagcagagtg cgctcagggc gcgcctccgg cgcgggcgcc
      541 gaatcttcct cgaagccaaa ccgggcaaat gggttttctg tggtggcgtt ttgttctggc
      601 gcgggcaagt cttcggcata gccgaatttc tcaaaaggat ttggcatcta aattacattc
      661 cgtctggcag gtagccgtaa aattcttgga atttgtcttt taaggatggg tttttgcgca
      721 actcgtctat cgctgcttgc ggggcttttc tgggctgccc ggttggtgtt ggtgctggct
      781 gatccgattt tgcctgagtg agcgccttaa ttttttgcct tgcattaatc gcctgggtaa
      841 tggcgcccgg aagcttctgt tccgggttct tgtcatccgc ataatcctcc ataggtattt
      901 gcgattggcg cgaaatagcc atggccatta gctcggtatc cttggcgtcg ttaccggttc
      961 gctgctttcg ataagccagc gcctcgtcag gtgtcatccc gagtgttttt aggtattcaa
     1021 cctctttttc aagggaagat tttgagctgg cgccagcgcc tgatttttgt ggcgccttga
     1081 caggatttcc tttcgagtcc agcacggggg tcgctaccgc gccacccgag cggctgggca
     1141 actgcatcaa attgccctcg ccgtcagccc ggtaggtaac cggatcaccc tcgttaattt
     1201 cttttgtgag ttttttaact tgaagattct tgatggtttc ttggtcggcg ctctttttaa
     1261 gctcttcgcc aatggtctgc tgaataaacc acttttggtt ttctgggtca gacaaaaacc
     1321 cacggtactg ctcagcaact ttgagccggc cgaaaaggtc ttctagtttc acctccttaa
     1381 ccgggtcgtc agggtcagaa ctgcggcgct cagtcagcgg cctttcttct gggtcgccat
     1441 ccactttaag cccaaccatt agcgttccgg gtgtccggcc aggataaagc cgggaggtgg
     1501 ataccttgcg cccttgcgtg gcacccaagc ctatctcagg gttcagcaca tcaaaggctg
     1561 cggccacttc tgggctgcgg tagtcaaccc tgccctcagc ggcgcccttc agggtttcaa
     1621 tggcctgccc gtactcaggg cttaaaattc tgcctgcatc caggtttggg tgagtcgcca
     1681 caaagcttcg atactcctcg ggggcgtcct ttagggagtg catcgaccag gccagggcct
     1741 tgttcatttt cgccacacgc tcatcgttta ggtcgcgcac ccgaatagca ttaatttggc
     1801 tttgctgctg ggcattttcc atttgcgcag cacgagcctc ctgggcgcgc tgctcttgag
     1861 cctgccgcaa ggatgccaaa ccggccctct cttcgcgctg cgcctgcaat aagcctgcgc
     1921 gctgctggcg atcctgcata ttgctcacaa actcagcgcc ccgcatggcg ccatccataa
     1981 aatttaaacc gcttaaaccc gccataaaat ccccgttaac tcagtaagta tccgccaagc
     2041 aacccaattc cgccgccaat cagggcgcca acagggccgc cagccatcgc cccatacatg
     2101 gcgccagtcg ccgccccggt gcccatgctc attttttccg tttggctttt ttgctccttc
     2161 aaagcccgat tcgcttgatt gcgccgctcc tcaagcattg ccgcgtctct cagcccgcca
     2221 agcgcactat tttttacact gctgctcatc tgcattaagc cgtaaccgct cattgctgtg
     2281 cacctcttaa gctggatagt ccgcccgtta gcaccgcctt gtctcggtct tgtgcatgta
     2341 gtcgggcttt attggtcgcc cccaccaggg aggcggtttt gagtgttgcg cccaggcgct
     2401 tttcgtctgc cgcttggtca ctgccgatgc ttacgccaag tcgagatcga tcccgcgcca
     2461 gcgtcccaag ggatgaatta aaagaatttt ccacagcccc ggccgcccgc tgttcgcttt
     2521 gcaggttgtc ggcattgcca gtagaaaggg cgattaactg atcctcgtag ggggcaaagg
     2581 tgttcttgta atctgcccac tggtcgcggg ttatttgcgc gaatgtgtcg gatgccgacc
     2641 taccgccaag ccctgaaaca ttttttatcc agtcagcaac tggcgctttg ccctgagccg
     2701 cagtgctcag ttgagataag cccgccatgt aaccccctta tgcgaacgga tcggaagagc
     2761 caaggctgtc gccaaagcca tattgcgcat ttgttttttt ctgccaggcc ttcttgtcat
     2821 tgagcccacc cccgtaaagt tgcgccccgg cacccaccag cgttccagcc gcagacatat
     2881 tggccgcccg gttgttgaat tgattggtaa catcttgcgt ggccttctgg tttgccttta
     2941 tcgctaggtc actaaggccc gcgcttgcct gggtcgcttc gccccggccg attgatacaa
     3001 tgtcctgcag gccgccaacg tgcatatcct cttgcgagag cgaggcgccc tgagtattgc
     3061 cggccgtgtc ttttgccctg gccatggcca gcgtaaaccc gcctgaagta ttggccgggg
     3121 aagaggcgcc aacacctggc ttgaccatgt ttaagccgcc atacttggcc tttgtggcgc
     3181 tggcggccgt gcccctggca aatgagtttg caggctcggt acccgaatcg ttaacggcct
     3241 taaaaaatgc ctcgcgggcg ggaatgccaa cctccttgta tcgcgcccac tgctcgttag
     3301 ctacttgggc aagcgctttt tcctgctcag tctctttttg ctggggggcg ctacccttat
     3361 aaaaaacccg ctgcgcacca aacttacgga agcgggccat aggctggaag cgtggcggct
     3421 caatcaagca accatcaagc aaatcaatca cagtgttttt ctccaaacgg ttacgggtac
     3481 gccccgccaa atatcaggcc ttggcgaata gcccaatgag cgcgcaaccc ggttaaagcc
     3541 agggcgcacg gtccaaaatt cgatacattg cccgccgatt tcgcgcacca agtcctcaat
     3601 ctgggactgt acggcgaggc ggtttacagg ggagtaagag gcggcagccc aaacaagcac
     3661 ggagctgggc ctgggtcgaa gaacaaaaaa gcctttctcg aaggcgaaaa aaaaggcccg
     3721 ctttttaagg caggcctttt ttatgtcgct gaataattca gggtgattca ccctatcgcc
     3781 aatgcgatta atcctgtcct tccaccggtt gagcgtatcg accgggggaa tatgaacgga
     3841 atacacggaa tgctcgcagg attcgacagc ttaggaattt tcccactttc gcgaaaaaaa
     3901 agctacgggg ctggatctgg cggcgggtaa cgatcgacaa gcgcccaggt ctcgccatcg
     3961 aaaacagctt tcagatcctc gctttccgtc ggtggctcca cggtggttat gtcggttctg
     4021 taagccgcct cttcggcggt taagtggagg cgagagatta aaaatccgct cgcgccgaaa
     4081 cagtaaaaaa tttgtctcat caagtaatcc tatgcagtgc ccagctaatt tggttggctc
     4141 taatgtttgt tacctgggga tgaacgtcag gattcaacct gacggtaata tataaaatta
     4201 tgcgcttttg cgattggtct ggatctgccc ccagtggccc agcccatgtg cgcatgaatc
     4261 caaccgagca ttccgcgtca agataaaacg cagtgcccga aaacgcgcct ccgctataag
     4321 agtaacttgc cgtaaaaact tgcgccctac caacaaagct tgccgataaa ttatcggtta
     4381 tgtcatccgt cgttatatag ccggtatcca ccgcaaaagt gacgccgccg ttctttccgt
     4441 cgtgagcagc agcataaacg ctttgctccg tgtatgtgcc atcatatatc aggtcgttgc
     4501 ccctgacata aaaccatctg tttccagccg atggagccga gacataaact ttctcaaaat
     4561 gccacccggc aggaggataa tagttgcccg ctgatccaga atcggcaact acatttgccc
     4621 tagtaatcac caccccgtta aattccgccg ccccactttt ctgaatctgc cacccagcat
     4681 tgccagggtt gtaattggat gagcttagcg tcccgcgagc aacgaggtcg ttaaactcag
     4741 cattaccatt gttgtgtatt ttccacccag aaacccctgc cgaataattt gacgactgaa
     4801 tgccctgaga tacgcttcct tcatcaatat ttgcctgccc agcatcaaca gtaataaaac
     4861 tggccttgag ccctgttatt ttccctgccg taacgcctcc aacctgcaga tcattgatag
     4921 tggcattttt tataaaagcc ccatccatca ccacctgccc ggttgtcgta tcaacaatga
     4981 acgggtaaat gtcactgccg gtcgcgcccg gttttccgat tgcgaattta tcggcgttga
     5041 taatgaacgt gcttgcagta ttggttgacg ccaggccgaa cccggacacc cggccattca
     5101 cgtcaatttt tagcgtgtac tgcccgtaca gaccggtaat cgagctggcc tgggcatcaa
     5161 acttctgctc aatggtgagg ccgccaacgt tgtttaggcg ggcggtaatt gcagtgtaat
     5221 caacggccga tgcggc
//
LOCUS       DAOQGS010000278         5226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8143_length_5226_cov_4.458712, whole genome shotgun sequence.
ACCESSION   DAOQGS010000278 DAOQGS010000000
VERSION     DAOQGS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_5226_cov_4.458712"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKZ52_10980"
     CDS             <1..927
                     /locus_tag="PKZ52_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60339.1"
                     /translation="LPRRTAALRHAILIAEKESHSQILNKTNWDLLAARYQQLLDYNK
                     AYDSGSASGEGEVEQLREELETSRKRISNLEKFKSLYFDLEKTWRESKGKAGEYFDEM
                     SSLISQGRPAEDLAPILNNYHTVYSGFSDQIEAGVDTNPIKYDEQSHKEIIRLRNVTV
                     DQHRTINELQEKLSRATNEEQRASVIKDLQSELQKQARYMQESETCIKLMEEELRNSK
                     KEIEQLRVRAAQVGQFRTRLKELQSAEEANQHIIQSLKQDNRRLNQKIKTINEAAPED
                     NNETRALRRELTTFKAKYAELEERYLDLKLKG"
     gene            1120..2505
                     /locus_tag="PKZ52_10985"
     CDS             1120..2505
                     /locus_tag="PKZ52_10985"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNG60340.1"
                     /translation="MSDASSHSSFHTDGGDFSFRQHHIRALVCFAAAGTSFSHINWQA
                     INWSLVGITIFVVLYSLVSYLYLKRYSSNQGRSFDRFMTADAAMIGLVLNFTEFRLLP
                     TILFVTMVQFNSLINGGLRKWAIDDAAMIGGMVVGFFLKAPQWVYSDRLEINSLSLIG
                     IFTYFCAYGLYAHKRMKKLDNHIKKLTSEHTLYKLRSYKLARYLTPTVWKAINEGKEH
                     TLTTDRKRVTVFFSDIMGFSALSEELEPETLTELLNSYLTEMARIATKHKGTIDKFMG
                     DAVMVIFGDAQSEGLKMDCLRCVSMAIEMRKKMLELQVRWSAIGIKNPLKIRMGINTG
                     YCTVGSFGTSHYMDYTVLGTHVNLASRLESAAEPGQILISHDTWSLVKDMVMCQDKGE
                     IPIRGFNQPVKVYQVDDFRKDLGKENSWMEYTSEGFSLHLDMEKLQSYQKDKVIEILR
                     KASEQITDRPT"
     gene            complement(2533..3336)
                     /gene="queF"
                     /locus_tag="PKZ52_10990"
     CDS             complement(2533..3336)
                     /gene="queF"
                     /locus_tag="PKZ52_10990"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210338.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNG60341.1"
                     /translation="MQTVYGKPVHLGLQQAYSDQYDPALLQPIERSSCRALLPIKEFI
                     GADLWTAFEVSWLDALGKPCVAALEFEFPANSNHIIESKSFKYYLNSFNQTRLASREE
                     LNQRLLNDLSLASGGQVAVRDYVPPVALGFDRQVLLDALPLVEPAYKPAPQLLSASGA
                     QVQEVLCSHLLKTNCPVTGQPDWASIWIGYEGAQIDHHGLLAYIVSFREQQDFHENCV
                     EHIYCDLWQRCAPVRLWVYARYTRRGGLDINPLRSSEPMAAPKLRGFRQ"
     gene            complement(3349..4122)
                     /locus_tag="PKZ52_10995"
     CDS             complement(3349..4122)
                     /locus_tag="PKZ52_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG60342.1"
                     /translation="MNMQQQFIAFSTIVRKEVRRFTRIWVQTLLPPAITMTLYFVIFG
                     HLIGSRIGQIHGFDYITFVVPGLVMMAVINNAFANVVSSFYSAKFTKSIEELQVSPTP
                     NSIILWGYVMGGVLRGLGVGVIVTTLSLAFTHLSIAHVGVTLTVVVLTSVLFSIAGLI
                     NAVYANSFDDISVVPTFILTPLTYFGGVFYSIDMLSTTWQWVSVFNPILYMVNVFRFG
                     ILGVSDVPVGPSLLGIGLLTLGLYIYCWYLLEKSGKLRG"
     gene            complement(4119..5060)
                     /locus_tag="PKZ52_11000"
     CDS             complement(4119..5060)
                     /locus_tag="PKZ52_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG60343.1"
                     /translation="MTAQPWALSIRDLTKTYSNQFTALKGINLDVAPGDFFALLGPNG
                     AGKSTTISIISSLVKKSAGSVSIFGCDIDRDFSAAKQHLGVVPQEFNFNLFEKVEDIV
                     ANQGGYYGLPRALAYQRAEKYLTRLGLWDKKNQQARNLSGGMKRRLMIARALVHEPRL
                     LILDEPTAGVDIELRRSMWDFLQEINEQGTTIILTTHYLEEAEMLCRNIAIINQGNIV
                     ENTSMKALLKQLDKETLVFDTRNPFGSLPVVADYLLRGVDEHSFEVVINKGQSLNPLF
                     AACTAQGIDIISMRNKVNRLEELFVALVQNHNGAGSL"
BASE COUNT         1500 a         1357 c         1203 g         1166 t
ORIGIN      
        1 ctcccccgcc gcaccgccgc cctgcgccac gccatattaa ttgcggagaa agaatcccac
       61 tcgcagattc tcaataaaac caactgggac ttgttagccg cgcgctatca acaactgttg
      121 gattacaaca aggcctacga cagcggctcg gccagtggcg agggggaagt tgagcaattg
      181 cgcgaagaac tggaaaccag ccgcaaacgc atttccaact tggaaaaatt caaaagcctc
      241 tatttcgact tggaaaaaac ctggcgcgaa agcaagggta aggccggcga atatttcgac
      301 gaaatgtcga gcctgatcag ccaggggcgc ccggctgagg accttgcgcc catcctcaac
      361 aactaccaca cggtttactc aggttttagc gatcaaatag aagcgggcgt cgataccaat
      421 cccatcaaat acgacgagca atcccacaaa gaaatcatcc gcctgcgcaa tgtcacggtg
      481 gatcagcatc gaaccatcaa cgagctgcag gaaaagcttt cgcgcgccac aaatgaagag
      541 cagcgcgcca gcgtgatcaa agatttacaa agtgaattac aaaaacaagc ccgctacatg
      601 caagaatcgg aaacctgcat taaattgatg gaagaagaat taagaaattc gaaaaaggaa
      661 attgaacaac tgcgggtgcg cgccgctcaa gtggggcaat ttcgcacgcg gcttaaagaa
      721 ctacaatccg ccgaggaggc caatcaacac attattcaaa gcttaaaaca ggacaatcgc
      781 aggctaaatc aaaaaataaa aaccatcaac gaagcggccc ccgaggacaa taacgaaacc
      841 cgcgcactgc gccgcgaact caccaccttt aaagccaaat acgccgagct tgaagagcgc
      901 tacctcgacc taaaactcaa aggctgaatc cgctgcgcga gcggcgatgg acagccctag
      961 aaaaatagcg tttaatgcac acccagaata ataatagcgt cacaaagttg acatcgtttg
     1021 tttgtctata aatacgaaaa ggcgcctaca cccccgatgt agtcaaccgc caacacacga
     1081 ttaatcgaaa aaataagctg aaattttagg gtgcgcacca tgagcgacgc ctcgtctcat
     1141 tcgagttttc ataccgatgg gggagatttt tccttccggc aacaccatat tcgcgcgctg
     1201 gtttgcttcg ctgcagcagg cacaagtttt agtcatataa attggcaggc cattaattgg
     1261 tctttagtcg gcattactat ttttgttgtg ctttactccc tcgtcagtta tctctattta
     1321 aaacgctact cctccaacca aggccgctcc ttcgaccgct ttatgaccgc cgatgcggcg
     1381 atgattggcc tggtgctcaa cttcaccgaa tttcgtttat tgccgactat tttatttgtc
     1441 accatggtgc aatttaatag cctgattaac ggcggcttac gcaaatgggc catagatgat
     1501 gccgctatga tcggcggtat ggttgtgggg tttttcctca aagcgcccca gtgggtgtac
     1561 agcgatcggc tggaaataaa tagcctcagc ctaattggca tttttaccta tttttgcgct
     1621 tatggtcttt acgcccacaa gcgcatgaaa aaactcgaca accacattaa aaaacttacc
     1681 agtgaacaca cgctgtataa attgcgcagt tacaaactcg cccgctattt aacgcccacg
     1741 gtttggaaag ccatcaacga aggcaaagaa cacaccctca ccaccgaccg caaacgggtc
     1801 acggtgtttt tctcggatat tatgggcttt agtgcgctct cagaagaatt agagccggag
     1861 actctcaccg aattattgaa ttcttattta accgaaatgg cccgcattgc caccaaacat
     1921 aaaggcacca tcgacaaatt tatgggcgac gctgtgatgg tgatttttgg cgatgcgcag
     1981 agtgaaggcc ttaaaatgga ctgtttacgc tgcgtctcca tggccatcga aatgcgcaaa
     2041 aaaatgctgg agttacaggt gcgctggtcc gccatcggca ttaaaaaccc gctgaaaatc
     2101 cgcatgggaa ttaacaccgg ttattgcacc gtcggctcct tcggcacaag ccattacatg
     2161 gattacaccg tgctgggcac ccatgtgaat ttagcctccc gcttagaatc tgccgccgag
     2221 ccggggcaaa tattaatttc ccacgacacc tggtcgctgg tgaaagacat ggtgatgtgc
     2281 caagacaaag gggaaattcc cattcgcggc tttaaccaac cggtaaaagt ctaccaagta
     2341 gatgattttc gcaaagactt gggcaaagaa aacagctgga tggagtacac ctcagagggc
     2401 ttttcactgc atttggatat ggaaaaattg cagagttacc aaaaagacaa agtcatcgaa
     2461 attctgcgca aggcctccga acaaattacc gaccgcccca cataaactcg ataactgggg
     2521 atttttgcgc aattattgcc gaaacccccg cagcttaggc gccgccatgg gctcgctact
     2581 gcgcagcgga ttaatgtcta acccgccgcg ccgagtataa cgagcgtata cccataagcg
     2641 cacaggcgcg cagcgctgcc aaagatcgca gtaaatatgc tctacacaat tctcgtggaa
     2701 atcctgctgc tcgcgaaaag acactatata agccagcaag ccatggtgat caatttgcgc
     2761 gccttcgtag ccaatccaaa tactcgccca atccggctgg ccggtaactg ggcaattggt
     2821 ttttaataaa tgactgcaga gcacttcttg aacctgggca ccgcttgcgc ttaataattg
     2881 tggcgccggt ttgtacgccg gctcaacaag cggcagtgca tcgagcagta cttgccgatc
     2941 aaagcccaac gctacaggcg gaacataatc gcgcaccgcc acttgcccgc cagaggcgag
     3001 ggataaatcg tttaacagcc gctgatttaa ttcctcccgc gaggccagtc tggtttgatt
     3061 aaatgaattc aaataatatt taaacgattt ggattcaatg atgtggttgg agttagccgg
     3121 aaattcaaat tccagcgccg ccacacaggg tttacccagg gcatccaacc aggacacctc
     3181 aaaagccgtc cataaatcag cgccgataaa ttctttaatc ggcaacagcg cgcggcagct
     3241 gctgcgttca atgggctgca aaagggccgg atcgtattgg tctgaatagg cctgctgcag
     3301 ccccaagtgc accggtttgc catagacggt ttgcatgttt acactcccct agccgcgcaa
     3361 tttgccgctt ttttccagca aataccaaca ataaatataa agccccagcg tcaataaacc
     3421 tatgcccagc agcgatgggc cgacgggtac atcgctgaca cctaagatgc caaagcgaaa
     3481 tacattgacc atgtataaaa ttggattaaa cactgaaacc cactgccagg tggtggacaa
     3541 catatcaata gaataaaaca caccgccaaa ataggttaag ggcgtaagaa taaaggtggg
     3601 caccaccgaa atatcatcaa acgaattagc gtatacggca ttaattaaac cggcgatgga
     3661 aaataacact gaggtgagca ccaccacggt taaagtcaca cccacatggg cgatacttaa
     3721 atgcgtaaag gcgagggaaa gtgtggtcac aatcacgccc acacccaagc cgcgcagaac
     3781 cccgcccatc acatagcccc acagaataat actgttgggg gttgggctca cctgtaattc
     3841 ttcaatactc ttagtgaatt tcgcgctgta aaaagacgac accacattgg caaaggcatt
     3901 gttaatcacc gccatcatca ccagccccgg caccacaaag gtgatgtaat caaagccgtg
     3961 aatttggccg atgcgcgagc cgattaaatg gccaaaaatc acaaaataca gcgtcatggt
     4021 gatggccgga ggcagcaggg tttgcaccca aatgcgcgta aagcggcgca cttctttgcg
     4081 cacaatggtg ctaaaggcaa taaattgttg ctgcatattc ataggctgcc tgctccattg
     4141 tggttttgca ctaaagcgac gaagagttct tcaagccgat tgaccttatt gcgcatacta
     4201 atgatatcta taccttgcgc ggtgcaggcg gcgaataatg gattgagcga ttggccttta
     4261 ttgatcacca cctcaaacga gtgctcatca actcccctca gcagataatc agccaccacc
     4321 ggcaaggagc caaaggggtt gcgtgtatca aacaccaggg tttctttatc cagctgtttt
     4381 agcagcgctt tcatactggt attttccaca atattgccct gattaataat ggcaatattg
     4441 cggcagagca tttccgcctc ctctaaatag tgggtggtga gaataatagt ggtgccctgc
     4501 tcattaattt cctgcaaaaa atcccacatg gagcggcgca gttctatatc taccccggcg
     4561 gtgggctcat ccaatattaa taagcgcggt tcatgcacca gggcgcgggc gatcatcaag
     4621 cggcgtttca taccgccgga tagattgcgc gcctgctgat tttttttatc ccacaatccc
     4681 aagcgggtga ggtatttttc cgcccgctga taggcaagtg cccgcggcag cccataatag
     4741 ccgccctggt tggccactat gtcttccact ttttcaaaca aattaaaatt aaattcctgc
     4801 ggcacaaccc ccaggtgttg tttggcggca gaaaaatctc ggtctatatc acaaccaaaa
     4861 atactcaccg agcctgcgga tttttttacc agagacgaaa taatactgat ggtggtggat
     4921 ttaccggcgc cattagggcc taagagggca aaaaaatccc ccggcgccac atccaaatta
     4981 atgcccttaa gggcggtaaa ttgattgctg taggttttag tcagatcgcg aatactcagc
     5041 gcccagggtt gtgccgtcat aaccacctcg tgtatctgcg tcgaaaataa atagtgctta
     5101 cgtcaaagaa gatttggaaa gaatccacag caaaaaaaaa agcgcagacc gtaatctgcg
     5161 ctttttgtat ttggagcggg aaacgaggct cgaactcgcg accccaacct tggcaaggtt
     5221 gtgctc
//
LOCUS       DAOQGS010000279         5224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8151_length_5224_cov_3.808280, whole genome shotgun sequence.
ACCESSION   DAOQGS010000279 DAOQGS010000000
VERSION     DAOQGS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8151_length_5224_cov_3.808280"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /gene="ovoA"
                     /locus_tag="PKZ52_11005"
     CDS             complement(<1..1554)
                     /gene="ovoA"
                     /locus_tag="PKZ52_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNG60344.1"
                     /translation="MLLTTDLPTHQGSTAASHTKTWHLLRTPNLRNPNLAQLRQSVRD
                     YFCNTFAVYDDLFTCLRTDAAYYEKPIALRHPLIFYLGHTATFFINKLVLARLIDTRI
                     NPRFESLFAVGVDEMSWDDLGVELNAWPKVAEVKAYRRHVRELVLSLIDTLPLTGPID
                     WQSPWWAIVMGIEHERIHLETSSVLIRQHQLHHVQPQKNWPVCPSAGAAPTNYLVPVT
                     GGVVHLGKKFDAPFYGWDNEYGEHSTQVRDFSAARYLVSNAEYKTFVDAGGYSNSQYW
                     DEEGQGWLSYTGAIYPTFWREKNGEYFLRTMTEEIPMPWNWPVEVNCLEADAFCRWKS
                     AELGQTLRLPTEDEWYRLYEVAGCSEVGELAAGNLHLDYFASACPVNQFAQGDFYDVT
                     GNVWQWTQTPMYPFEGFEVHPLYDDFTAPTFDNRHNLIKGGSFIACGNESLKSARYAF
                     RRHFFQHAGFRYVVGDEVNQVPQSHYETDKLMSEYAEFHYGDEYFAVPNFPAALVNWV
                     RPYLASKPRS"
     gene            complement(1780..3117)
                     /locus_tag="PKZ52_11010"
     CDS             complement(1780..3117)
                     /locus_tag="PKZ52_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNG60345.1"
                     /translation="MSDPISPLIDALLEDPGSETQRQLQEALKPYTVDELALVLESLP
                     LAKRVIAWHHIPQEMRLDVLVALRGEARLLLLRNLPSDELPSLFEDLEAEDLLELAES
                     LPESTIDLALSRMDERQRQSYALAQSFSEQQIGRWMNNHVLVVAQNMRVAEALRLLRR
                     EAPKHTEVLYLVNRTGRWVGCVRISQLLTVLPHTSVGELQEEDFPSLNAEDNLYEAAE
                     QVAYSGYSALPVLNKEGVLLGRIDMSTAVELLHEQAERQRMSQAGLAEDADLFAPAKR
                     SARTRALWLGINLLTALLASWFIGLFGHSIERVVALAVLMPVVASMGGIAGSQTLTLV
                     VRGLALGQVHTSNLAALFDKELRVGAINGVLWALVIGYVAWLWFGSLLLGLVIGTAVV
                     VNIVTAAVFGVLLPVLLDRLRIDPALSGSVILTTITDIIGFVAFLGLGTLLLV"
     gene            3308..4702
                     /locus_tag="PKZ52_11015"
     CDS             3308..4702
                     /locus_tag="PKZ52_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNG60346.1"
                     /translation="MSAQPSSPQVHKLSSGQLLSLALPVALQMLMQSALGMVDVMMVA
                     PLGATALAAVGLAAKLHFLTLVLQSGIATGCSVLVAQYLGARDMANCRSILAAALWTG
                     LGFSLPFTLACAFWAQSWMPFINPDAQVCAIAAEFMAITALALLFTQLIVIYEGALRA
                     QGQTGLPMLAALVSIATNCALNYGLIFGKWGLPELGVLGAAWGTMLARTVQLMLVMGW
                     VYLRPSGFNLSLTQLLHACHPERLKYFLRFSAPLMANYALWGLGNTCYHALTGFAGTQ
                     ALAAMAVMVPIESGLFALFVGLANASAVLVGRSLGANEFKYAWYLHHYFDKLTLVLLL
                     LLCSALWLLHEPLLALFAPSPEVAEVLKHTLALFCLLVWIKVLNMVRIIGVLRAGGDN
                     QFCLITDTIVMWGIGLPVFAVCVFIWPQSFFILYCLMFLEDALKFVPVWRRIYRRRWL
                     TNLTTAHPRGEIHV"
     gene            4695..4943
                     /locus_tag="PKZ52_11020"
     CDS             4695..4943
                     /locus_tag="PKZ52_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNG60347.1"
                     /translation="MSDTPKARPKDPLEGVSLEQILNRLQAHYGWAALGALIDIRCFK
                     QDPSIKSSLTFLRRTPWARSKVEDLYRQLVINGRLDGA"
     gene            complement(5016..>5224)
                     /locus_tag="PKZ52_11025"
     CDS             complement(5016..>5224)
                     /locus_tag="PKZ52_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60348.1"
                     /translation="REIDAERRDLRDQRRDLDDQLGRFDDGRERSFWYNNREYLLDER
                     GYCYRVTRSGNSRRMSRVSSSYCR"
BASE COUNT         1094 a         1543 c         1480 g         1107 t
ORIGIN      
        1 gctgcggggt ttactggcaa gatacgggcg cacccaattg accagcgccg cagggaaatt
       61 gggtacagca aagtattcat cgccgtagtg gaattcggca tattccgaca ttaatttatc
      121 ggtttcatag tgggattggg gcacttggtt gacctcgtcg ccgacgacat aacgaaaacc
      181 tgcgtgttga aaaaaatgcc gccggaaggc gtagcgggca gattttaatg attcgttgcc
      241 gcaggcaata aaagagccgc ctttaattaa attatggcgg ttatcaaagg tgggggcggt
      301 gaaatcatcg tacagcggat gcacttcaaa gccttcaaat gggtacatgg gtgtttgtgt
      361 ccactgccaa acattgccgg taacatcgta aaaatcccct tgggcaaatt gattgaccgg
      421 gcaggcggag gcgaaataat ccaggtgtaa attaccggca gccagttccc ccacttcgct
      481 gcaaccggcc acttcgtata aacggtacca ttcatcctcg gtgggcaggc gcagggtttg
      541 gccaagctca gcggatttcc agcggcaaaa ggcgtcggct tccaggcaat taacttccac
      601 cggccaattc cacggcatgg gaatttcttc agtcatggtg cgcaggaaat attcaccgtt
      661 tttctcgcgc cagaaggtgg gataaatggc gccagtgtaa cttaaccagc cctgcccttc
      721 ttcgtcccaa tattggctat tactgtagcc gcctgcatcc acgaaggttt tgtattcagc
      781 gttgctcact agataacggg cggcggaaaa atcccgcacc tgggtgctgt gctcaccgta
      841 ttcattatcc cagccgtaaa aaggcgcatc gaattttttg cctaaatgca ccacaccgcc
      901 agttacgggc actaaataat tagtcggtgc agcgcccgca ctcgggcaaa caggccaatt
      961 tttttgcggc tgtacatggt gtaattgatg ttggcgaatt aataccgagg aagtttctag
     1021 atgaatgcgc tcgtgttcta tacccatcac tatggcccac caggggcttt gccaatcgat
     1081 ggggccggtg agcggcaggg tgtcaatcag gcttaacact aattcacgca cgtggcggcg
     1141 gtaggctttt acctcggcaa ctttcggcca agcgtttaat tccacgccta aatcgtccca
     1201 gctcatttca tccacgccca cagcaaatag ggattcaaaa cgcgggttaa tgcgggtatc
     1261 aattaaacgg gcgaggacta atttattaat aaaaaaagtt gccgtgtggc ccaggtaaaa
     1321 aattaacggg tggcgcaggg cgatgggttt ttcgtaatag gcggcgtcgg tgcgcaggca
     1381 ggtaaataaa tcgtcgtata cggcaaaggt gttacaaaaa taatcccgca ccgattggcg
     1441 cagctgggca aggttggggt tgcgcaaatt cggcgtgcgc aataggtgcc aggtttttgt
     1501 atgagaagcc gcagtagagc cttggtgtgt gggtaggtca gtggttagca acacgcatca
     1561 ccccttatgg tatgaatgag caagtgggaa accggccttg gcagccgccg gcgcgaacag
     1621 aatggggtta tacgagcaaa cagggtgttt tgatcaatct ggcccctagc agcagcccta
     1681 cccaaaccga aactcggccg gattctacca ccggttttgc cagtaccggt tcataaaatc
     1741 cactgggttt gacttagccc atagccgcag ccaccgctgc tagaccaaca gtaaggtgcc
     1801 caaacccaga aaggccacaa agccgatgat gtcggtaata gtggtgagga tgacggagcc
     1861 tgacagggcc gggtcgatac gcaggcgatc caacagcacc ggcagcaaca cgccgaacac
     1921 cgcagcggtg actatattga ccaccacggc ggtgccaatc accagcccca gcagcaggct
     1981 gccgaaccag agccaggcca cataaccgat taccaaggcc cagagcacgc cgttgatagc
     2041 acccacccgc aactctttat cgaacagggc agccaggttg gatgtgtgca cctgccccag
     2101 ggccaggccc cgcaccacca gggtaagtgt ctggctaccg gcgatgccgc ccatggaagc
     2161 caccaccggc atcaataccg ccagggccac tacccgctcg atgctatggc cgaacaggcc
     2221 gataaaccag gaggccaaca gggcggtgag caggttaatg cccagccaga gggcgcgggt
     2281 gcgggcggag cgtttggccg gggcgaagag atcggcgtcc tcggccaaac cggcttggct
     2341 catgcgctgg cgctctgcct gctcatgcaa gagttccact gcggtactca tgtcgatcct
     2401 gcccagcaat accccctcct tgttgagtac cggcagggcg ctgtagccgc tgtaggccac
     2461 ctgctcggcg gcctcataga ggttgtcctc ggcgttaagg ctggggaagt cctcctcttg
     2521 cagctccccc acgctggtgt ggggcagcac ggtaagcaac tggctgatgc gcacacaacc
     2581 cacccagcgg ccggtgcggt tgaccagata aagcacctcg gtgtgtttgg gcgcctcccg
     2641 ccgcagcagg cgcagggctt ccgccacccg catattctgg gccaccacta ggacgtgatt
     2701 gttcatccag cggccgattt gctgctcgct aaaactctgc gccagggcgt aagactggcg
     2761 ctgtcgctca tccatgcggc ttagggctag gtcgatggtg gattccggca ggctctccgc
     2821 cagttccaat aaatcctccg cctccaggtc ttcaaacagg ctgggtagct cgtcgctggg
     2881 caggttgcgc agcagtaata aacgcgcttc accgcgcagg gccaccagca catccaggcg
     2941 catctcctgg gggatgtggt gccaggcaat cacccgttta gccagaggga gggattctaa
     3001 aaccagcgcc agctcatcca ccgtgtaggg tttgagtgcc tcctgcaatt ggcgctgggt
     3061 ttcgctgcca gggtcttcta agagcgcgtc aataagtggg ctgatggggt ccgacatggc
     3121 tgtacctcag tatcgcagtg ggcggcgcag ggcgaacacc tgggccccat taacctaaag
     3181 catagaagta atcggcctaa aaaacccccg gctgtgacca atggcgctga taaggggcca
     3241 aacgcaggcc aagacgggta ttttccctta agatgccgcc cccagttgtt taagtaccgg
     3301 agttgttttg tccgcccagc cctcctcccc ccaggttcat aagctctcct ctggccaatt
     3361 gctcagcctc gccctcccgg tagccctgca aatgctgatg cagtcggccc tgggtatggt
     3421 ggatgtgatg atggtggcgc ccctgggggc cacagccctg gcggcggtgg gactggcggc
     3481 caagctgcac tttctcaccc tggtattgca aagcggcatc gccaccggct gcagtgtatt
     3541 ggtggcccag tacctaggtg cacgggatat ggccaactgc cgcagcattc tagccgccgc
     3601 cctgtggacg ggcctggggt ttagcctgcc cttcaccctc gcctgcgcct tttgggccca
     3661 gagctggatg ccattcatta acccggacgc ccaggtgtgt gccattgccg cggagtttat
     3721 ggccatcacc gccctggccc tgttattcac ccaattgatc gtgatttacg aaggcgccct
     3781 gcgcgcccag ggccaaaccg gtttacccat gctggccgcc ctggtctcca tcgccaccaa
     3841 ttgtgccctc aattacgggc taatttttgg caagtggggc ctgccggaat tgggtgtgct
     3901 gggggccgcc tggggcacta tgctcgcccg cacggtgcaa ctgatgctag tgatgggctg
     3961 ggtgtacctg cggcccagcg gctttaacct gagccttacc caattgctgc acgcctgcca
     4021 tcctgagcgg ctcaaatact tcctgcgctt tagcgccccg ctgatggcca actacgccct
     4081 ctggggcctg ggcaatacct gttaccacgc cctcaccggc tttgccggca cccaggccct
     4141 ggccgccatg gcagtgatgg tgcccatcga gagcgggctg ttcgccctgt ttgtgggcct
     4201 ggctaacgcc agcgctgtcc tcgtcggccg ctcccttggc gccaacgagt ttaagtacgc
     4261 ctggtacctg caccactact tcgacaaact cactctggtg cttttgctgc tcctgtgcag
     4321 cgcgctgtgg ctgctgcacg agccgctcct cgccctgttt gccccaagcc ccgaggtagc
     4381 cgaagtgctc aaacacaccc tggcgctgtt ctgcctgctg gtgtggatta aagtgctgaa
     4441 tatggtgcgc atcatcggcg tgttgcgggc gggcggggac aatcagtttt gtttaatcac
     4501 cgataccatc gtcatgtggg gcataggcct gccggtgttt gcggtgtgcg tgtttatctg
     4561 gccgcagagt ttttttatcc tttactgcct gatgttctta gaagatgccc tgaaatttgt
     4621 gcccgtgtgg cggcgcattt accggcgccg ctggctcacc aacctcacca ctgcccaccc
     4681 ccggggggag atccatgtct gacacaccca aagcccgccc caaagacccg ctggaaggtg
     4741 tcagccttga gcagattctt aaccgcctgc aggcccatta cggctgggcc gccctgggcg
     4801 ccctgattga tattcgctgc tttaaacagg atccgagcat taaaagcagc ctcaccttcc
     4861 tgcgccgcac cccctgggcg cgcagcaaag tggaggactt ataccgacag ctagtgataa
     4921 acgggcgctt ggatggggcc tgagctgatc caggccttca ccaacctagg cccacaaaaa
     4981 agccggccca cggccggctt ttgctctgct cacacttagc ggcagtagga gctggatacc
     5041 cggctcatac ggcggctatt acctgagcga gtaaccctgt aacagtagcc gcgctcatcc
     5101 agtagatact cccgattgtt ataccaaaag ctgcgctccc ggccatcatc aaaccgccct
     5161 aattgatcat caagatcccg gcgctgatcc cgcaagtcgc ggcgctcggc gtcgatttca
     5221 cggc
//
LOCUS       DAOQGS010000280         5223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8154_length_5223_cov_5.087268, whole genome shotgun sequence.
ACCESSION   DAOQGS010000280 DAOQGS010000000
VERSION     DAOQGS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_5223_cov_5.087268"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1253
                     /gene="gcvT"
                     /locus_tag="PKZ52_11030"
     CDS             147..1253
                     /gene="gcvT"
                     /locus_tag="PKZ52_11030"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNG60349.1"
                     /translation="MAEQPLLETPLADTHRALGGKLVDFGGWAMPVNYGSQIEEHHQV
                     RRHAGMFDVSHMTVVDVTGSDAKAYLQYLLANDVAKLTQIGKALYTGMLNFEGGVVDD
                     LIVYLTEFGYRLVVNCGTRAKDLAWMAKIGADFNVQISERPELAMIAVQGPQARSLVA
                     EVLQAPEVLDLAVFFGQPLSGVKADWFVARTGYTGEDGFEIMLPGTQAAEFWQALMAA
                     GVAPCGLGARDTLRLEAGMNLYGHEMDDTVSPLAANMAWTIAWAPAERDFIGRAALTA
                     QRTAGTDKLVGLVLKSRGVLRADYPVIIPAIAKPGIITSGTFSPTLGYSIALARIPSG
                     PGTEVGAKVQVLIRNSPVEVEIVKPCFVRNGQAV"
     gene            1485..1868
                     /gene="gcvH"
                     /locus_tag="PKZ52_11035"
     CDS             1485..1868
                     /gene="gcvH"
                     /locus_tag="PKZ52_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNG60350.1"
                     /translation="MSQVPAELKYAASHEWARLEADGTVTVGITDHAQAALGDVVFAE
                     SPALGSQVVAGKEAGVVESVKAASDIYAPISGEVVAVNSVLEDAPETINSEPYGAGWF
                     FKIKPSNPAELDALLSAAGYESSIS"
     gene            complement(1890..2444)
                     /locus_tag="PKZ52_11040"
     CDS             complement(1890..2444)
                     /locus_tag="PKZ52_11040"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNG60351.1"
                     /translation="MPLDQSIRFVAALCGLMVAGFVGSLITGGYLNQWGIHPRHLGSI
                     WTIFTSPFLHASLGHLLNNLAGFGVLAFLVMLRGKACFIQASLIIVVVGGLLVWLFGR
                     SANHIGASGWIFGLWGLLLAMAWFERSFTSIALAVVVIFFYGGMIWGILPLRRFVSFE
                     AHLFGLVAGVLAAYLWRKGLRLLG"
     gene            2472..3287
                     /locus_tag="PKZ52_11045"
     CDS             2472..3287
                     /locus_tag="PKZ52_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG60352.1"
                     /translation="MPDGHSPMIFNAAWPFDLTLNSVLIFILFIWSGFVRTGLGFGGA
                     LLTMPFILLIDNRPLIYLPVIALHLLIFSPLTLWQSRRQQPDAKHFFAQVDWAILKRT
                     LPIMLPAKLLGVIGIINLPSHIITPIIYGVVAIYALSYLLNKPIVSSKAWMDWPLLSL
                     GAYFSGTSLVGGPLIMAVYIGKVAKDNLRDTIFVLWFILVAIKMFSFVALGVDLQLAA
                     HLWALPAAFIGHVLGEYFHRFLIQREQKQFFQIVGGFLLVACTGGLIKQALNL"
     gene            complement(3680..4033)
                     /locus_tag="PKZ52_11050"
     CDS             complement(3680..4033)
                     /locus_tag="PKZ52_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNG60353.1"
                     /translation="MNTAFKAAALLGGTAVLAGAFGAHGLKAMLSEHNLEVWRTAVLY
                     QFFHALALLAITSVPQQKAWRLSVWAWILGVLIFCGSLYALALGAPSKLGMITPIGGL
                     ALCVGWFNLLRFNAQ"
     gene            4189..5205
                     /locus_tag="PKZ52_11055"
     CDS             4189..5205
                     /locus_tag="PKZ52_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HNG60354.1"
                     /translation="MALDFLSRTRSFFSALTLCSLLSACGADPAGQGLVGSSSSSSSS
                     SGKISSSSSSSSSSSSSSSSNSSSSSSSSSSSSSSSSSSSSSSSSSSSGQTAGYQRGD
                     YILGMDISYWSEQLANNARYVDVDGVSKDLLALLKNHGINFIRLRTFVDPKAAYGYAS
                     TINGCSGKPQAFLGKDDIVRTATRIKAAGMGFLLDFHYSDTWADPGKQIIPEAWRGAR
                     SIDQLALEVQRYTTGVLESLKAVNALPNMVQVGNEISPGMLIHTPGANTDCWGNNSVV
                     NAAVNGQANSTNWGNLARLLRAGSQAVKQVDSTILTALHIERTHSPSDVEWWMERATT
                     KIRI"
BASE COUNT         1286 a         1450 c         1321 g         1166 t
ORIGIN      
        1 cgcagcacca aagcactcac ttatcggcct ctagtcggcg aaaacttcag acccatccag
       61 aataaaaaag gcattcaata gctacgattt ttttatcctg ccaaggcaag cgcgttagaa
      121 tgccgagttt tacagcgggc atttttatgg cagagcaacc ccttcttgaa acccccttag
      181 ccgacaccca ccgcgccctc ggcggcaagt tggtggattt tggcggctgg gccatgccgg
      241 tgaattacgg ctcgcaaatt gaagagcacc accaggtgcg ccgccacgcg ggcatgtttg
      301 atgtctccca tatgaccgtg gtggacgtaa ccggcagcga tgctaaagcc tatttgcagt
      361 atctgttggc caatgatgtc gctaagctca cgcagatcgg caaagccctc tatacaggca
      421 tgctgaattt tgaaggcggt gtggtggacg acctgatcgt gtacctcacc gagtttggct
      481 accgcctggt agttaactgc ggcacccgcg caaaagactt agcctggatg gccaaaatcg
      541 gcgccgactt taatgtccag attagcgagc gcccggaact ggccatgatc gccgtgcaag
      601 gtccccaagc gcgcagccta gtggccgagg ttttacaggc ccccgaagtt ttagatttag
      661 cagtattttt cggccaaccc ttgagcggcg tcaaagccga ctggtttgtg gcccgcaccg
      721 gttacaccgg cgaagatggt tttgaaatta tgctgccggg cacccaggcc gccgaattct
      781 ggcaggccct aatggccgca ggcgtcgccc cctgtggcct cggcgcgcgg gataccctgc
      841 ggctggaagc gggcatgaac ctttatggcc acgagatgga cgacactgtg tcgcccctgg
      901 cggccaacat ggcttggacc atcgcctggg cgcccgccga gcgggatttt atcggccgcg
      961 ccgccctcac cgcccagcgc actgcaggca ccgacaaact ggtgggcctg gtgctgaaat
     1021 ctcggggtgt actgcgcgct gattacccgg ttattatccc ggctattgcc aagccgggca
     1081 tcatcaccag cggcaccttc tcccccaccc tgggctattc catcgctctg gcccgcatcc
     1141 ccagcggccc gggcactgaa gtgggcgcca aggtgcaggt gctaattcgc aacagcccgg
     1201 tggaagtaga aattgtaaaa ccctgttttg tgcgcaacgg ccaggcggtt tgaacctaaa
     1261 aaccgcagtt gaacccgtag cgagggtgct taagacgcct aatatgcgca gtttttggta
     1321 aaaattcggc gacggcagta gtcaccgcta cggtgagtcg aatttttacc aaaaactgtg
     1381 cagattcaaa tcttaggcag ccgcagtagg gttcaaatgc ggtgtttagg ttgaaatgcg
     1441 cgccccttta ctaatactga cccaattacc cctgaggaac tgatatgagc caagtccccg
     1501 ccgaattaaa atacgccgcc agccacgaat gggcccgctt agaagccgat ggcaccgtca
     1561 ccgtgggcat caccgaccac gcccaagcgg cccttggcga tgtggtattt gccgaatccc
     1621 ccgccctggg cagccaggtg gtagcgggta aagaagcagg ggtggtggaa tcagtgaaag
     1681 ccgcctctga catctacgcg cctattagcg gtgaagttgt cgcggtgaat tccgtgctgg
     1741 aagacgcccc ggaaaccatc aatagcgaac cctatggcgc cggttggttt ttcaaaatca
     1801 aacccagcaa tcccgccgag ttagacgcat tgttaagcgc cgccggttac gaaagcagca
     1861 ttagctaatg gccccaaacg aggctcagat taccccagta gcctgagccc cttacgccac
     1921 aaataggcag ccaaaacccc cgccaccagc ccaaataaat gggcctcaaa cgacacaaac
     1981 cggcgcaggg gcagaatccc ccaaatcatc ccgccgtaaa aaaagatcac caccaccgca
     2041 agggcgatac tggtaaaact gcgctcaaac caggccatag ccagcagcaa accccagagg
     2101 ccaaaaatcc agccgctagc gccaatatga ttggccgaac gcccgaatag ccacaccaat
     2161 aaaccgccca ccaccacaat aatgaggctg gcttgaataa aacaggcctt gccccgcagc
     2221 atcaccaaaa atgccaatac gccaaagccc gctaaattat tcagcagatg gcctaggctg
     2281 gcgtgtaaaa acggtgaggt aaaaatggtc caaatactgc ctaaatgacg cgggtgaatc
     2341 ccccactggt tgaggtaacc gccggtaatt aagctgccga cgaaaccggc caccatcagg
     2401 ccgcagagcg cggctacaaa acgaatagat tgatccaagg gcataaggcc tccacaatag
     2461 gaagcccgat tatgccagat gggcattcac cgatgatatt taatgccgcc tggccttttg
     2521 atctcaccct aaacagcgta ctgattttta ttttgtttat atggagcggt tttgtacgca
     2581 cgggcttggg ctttggcggc gccttgctca ccatgccgtt tattttatta atcgataacc
     2641 ggccgttaat ttatttgccg gttatcgccc tgcatttatt gattttctcc cccctcaccc
     2701 tctggcaaag ccgccgccaa caaccggatg ccaagcattt ttttgctcag gtagattggg
     2761 ccattttaaa acgcaccctg ccgattatgc tgcccgccaa attattaggg gttataggca
     2821 ttattaattt acccagccac atcatcacac cgattattta tggcgtggtg gctatttatg
     2881 ccctgagtta tttattgaat aaaccgattg tcagcagtaa ggcctggatg gactggccgc
     2941 tgttaagcct gggggcttat tttagcggca cgtctttagt gggcggcccg ctcataatgg
     3001 ccgtttacat aggcaaagtc gccaaagata acctgcgcga tactattttt gtgctctggt
     3061 ttattttggt cgccattaaa atgttctcct ttgtggctct aggtgtagat ttacaactgg
     3121 ccgcccacct ctgggcgcta ccagcggcat ttatcggcca tgtgttgggt gaatattttc
     3181 accgcttttt aattcagcgc gagcaaaaac agttttttca aattgtgggt gggtttttat
     3241 tagtggcctg cacaggtggt ttaattaaac aggcgctgaa tttataaact aaccaccgct
     3301 cactgccctt tcattctctc tgcaattaat cagactatgc ggcgcggcgg gcacccacaa
     3361 ggggtgcctc tattgcaaag gaagttaacc cggcggctca tcgatacacg gtgcagcagt
     3421 ggggaataca ggtaatttca gaccgtcaaa aagagggatc tttttgatgc gcctacaggg
     3481 atgtatttac ggcgtgtctg caattacctg tattcaccac tgcgggggac tagtgctggc
     3541 tcgcagcact aaagcattcg cctggttgct tctaaactag gggcattaaa tgccaagtta
     3601 acagacaaag cgaaaatccc gcccggttac tataaaaagc actgaatatt taagccttaa
     3661 acaaatctca agcggctatt tattgcgcgt taaatcgcaa caaattaaac caacccacac
     3721 acaaagccag cccacctatg ggcgtaatca tgcccagctt acttggagcg cctaaagcca
     3781 gagcatacag gctgccgcaa aaaatcagca cacccagaat ccaagcccag acgctgagcc
     3841 gccaggcctt ttgttggggc acactagtga tggccaacag ggccagggca tggaaaaact
     3901 gatacaacac cgccgtgcgc cacacctcta aattatgttc gctaagcatc gcttttaatc
     3961 cgtgggcgcc aaaggccccg gctaaaactg cagtgccgcc caatagggcg gcggccttaa
     4021 aggctgtatt cataatggct cccggatttt tagtggtcac gcgatttaaa agcggtgcaa
     4081 attagcattt tgcgagggag attgcgccgc aggaatatca tctgggtatt atggcaatta
     4141 actgacaaat accgagcctt gcatggctca gactaagagg acctcgccat ggctctggat
     4201 tttctcagcc gcacccgcag ctttttttca gcacttacct tgtgctcgtt gctgagcgcc
     4261 tgcggcgcag accctgccgg gcaaggactc gttggcagct ccagttcatc aagctcctcc
     4321 agcggcaaaa tctcaagcag cagttcctct agcagttcgt ctagctctag cagttcatca
     4381 aactccagta gctcgtcctc gtcgagcagc tcttcatcct caagcagttc gtccagtagc
     4441 tcgtccagct ccagcagctc ctccggccaa acagcaggtt accaacgggg ggattatatt
     4501 ttggggatgg atatttccta ctggtcggag caattggcca ataacgcccg ctatgtagac
     4561 gtggacggcg tcagtaaaga tttattagca ctgctaaaaa accacggcat taattttatt
     4621 cgcctgcgca cctttgttga ccctaaagcc gcctatggtt atgcctccac cataaatggc
     4681 tgcagcggca aaccccaggc atttctgggt aaagacgaca tagtgcgcac cgccacgcgc
     4741 atcaaagccg caggcatggg ctttttattg gattttcatt acagcgatac ctgggctgac
     4801 ccaggcaaac aaatcattcc tgaagcctgg cgcggcgctc gctccataga ccaattagcc
     4861 ctggaggtgc agcgttatac caccggtgta ttggagtccc taaaagcggt aaatgcgctg
     4921 ccgaacatgg tgcaagtggg caatgaaata tcgccgggca tgttaattca cacacctggt
     4981 gccaacaccg attgctgggg taataattcg gtggtgaatg cagccgtcaa cggccaggcg
     5041 aattcaacta attgggggaa tttagcgcgc ctattgcggg cggggtcaca agcggtaaaa
     5101 caagtggaca gcactattct caccgctctg catattgagc gcacccacag ccccagcgat
     5161 gtggagtggt ggatggaacg cgctacaaca aaaattagaa tttgatgtga tggccttttc
     5221 cgc
//
LOCUS       DAOQGS010000281         5209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8199_length_5209_cov_5.537059, whole genome shotgun sequence.
ACCESSION   DAOQGS010000281 DAOQGS010000000
VERSION     DAOQGS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_5209_cov_5.537059"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1409
                     /locus_tag="PKZ52_11060"
     CDS             270..1409
                     /locus_tag="PKZ52_11060"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNG60355.1"
                     /translation="MPMPSAFKSTLLACAIAALTACGADPITGNGASSSSSSSSSSSS
                     SSSSGRPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGTSGGFSCPANPGQA
                     PSGQLTASRIAGTASAQPGSGLYEGPVWIKGALYFSDFSFSNNFASRIQTLSASGVMS
                     TFITNSGSNGLAVDADGNILAATHDRKAISRYNLATKAREIVVGQYAGNPFNSPNDIT
                     VSSKGIIYFTDPDFQKSAAAGGQPQTRAYQYDGQTVKVIEENMYNPNGIALSPDESTL
                     YIAGNGMLKAYPVTSSGVGTGVNLASVTSPDGMAIDCLGNIYVAEHDQKRLKVMTPTG
                     TQIATINVDANVTNAAFGGSNGKTLYITGSGTLWKIDLNVVGKPY"
     gene            2084..3037
                     /locus_tag="PKZ52_11065"
     CDS             2084..3037
                     /locus_tag="PKZ52_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNG60356.1"
                     /translation="MEFAQIEITTRCNFTCGFCAGRHMPQRDFSRADFNAILAQWPNL
                     TAIELQGEGEPLIHPDFFALVNDAKHHCPDAVLSTISNGSLLNASNIEQLLQSPLDHL
                     WISLETLNPLDFKRLRGGKLSRVLRGLRSLLVAKRALAADLKIGFAVTLLHGHSHQLQ
                     AISRLYHALGMDGGISLQGLQTMPAYQQHYSAELIAELLQPQDNQVIKQLIASDSQVK
                     RALFDYARQGSFYARMYQKAGAHCCPWLTLGLYIDVQGRVMSCCFSKNPQQALGHFTQ
                     LTQVLKARRAMAQSLAAGTIPSPCSGCGIAQQLCSAKTLSV"
     gene            3327..4883
                     /locus_tag="PKZ52_11070"
     CDS             3327..4883
                     /locus_tag="PKZ52_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60357.1"
                     /translation="MHALTIFTRSVLILSLIALAACSGPDGSASSSSSSSSSSSSGRT
                     SSSSSSSSSSSSSSSSSSSSSSGSAINPVDAGRDQVVFNGEWVRLNSSNLPNTTLKWR
                     QLSGPSVNLDPQPATGFIAPDVRQSTDLSFELSAQINGQTFSDTVNVRVEPCKNADGI
                     VYGDCISPGFGPFLAYESATGTGEVFHYQGTETNRHVQWQSVDSGDSKHGKVMEIRWN
                     ANDPNHTINANGWFGLAMPGTSADLQAYKDGAISFDMRLTYHEQPNSAAPFIFKSECI
                     LPCVSAELTIDKGNSSFDWQTHTYPVADLVASGLNLSKVNHLWVIQPAWRNQEQNVTV
                     QIDNIRLLKTYTPPVPANGCTGSGKVTYTLSRAANPTADEQEAYNLITTAMDLAVKNY
                     NCYTNLSRQLQITYNPSVATADGSTNGSIRFGSRASMHHVTAMHEIAHVFGVGAAKFK
                     TLVSNGIYTGPKATAKLREISGVATDQLKSDGTHFWPHGLNYVSEWKNQQDLINHCSV
                     VQAIVADLAN"
BASE COUNT         1302 a         1443 c         1179 g         1285 t
ORIGIN      
        1 agagttcaac tgcggttttt aggataattc ttaacacctc acaaaaaatc tgcgctctat
       61 gtgcctgacg tcacttttta gctggctcta gcggtgcact atagttactt gattaacagc
      121 ctgtgacctg tgcccacaat tcagcttaac tgggccgcct gaaaaaccct cgcaggtaat
      181 atacttaatg cacttaattt gccggcgcgg ttaacctgcc gcggccaata cccaggtcaa
      241 tccacaccct atatctttga ggatcctcta tgccaatgcc ttctgcattt aaaagcacac
      301 tcttggcgtg tgctatagct gccctaaccg cctgtggcgc agacccaatt accggcaacg
      361 gcgcaagcag cagcagctca tcgagcagta gttcaagcag cagttcaagc tctggccgcc
      421 cctcaagcag cagctccagc tcatccagtt ctagttcttc ctcatcaagc tccagctcat
      481 ccagctcatc cagttcgtcc tcttccagtt catcaagctc cagctcctcc ggtacttcgg
      541 gcggttttag ttgccctgcc aacccaggcc aagcaccttc tggccagctc accgccagcc
      601 gtatcgcagg caccgcgtcg gcacagccgg gctcaggcct ctacgaaggc ccggtctgga
      661 tcaaaggcgc cctgtacttt tcagactttt ccttcagcaa caacttcgcc tcgcgcattc
      721 aaaccttaag tgccagcggc gtgatgagta cctttatcac caacagcggc agcaacggcc
      781 tggcggtgga tgccgacggt aatattctgg ccgccaccca tgatagaaaa gccatttccc
      841 gctacaacct tgccactaag gcgcgggaaa ttgtggttgg ccaatacgca ggcaacccat
      901 ttaattcccc taatgacata accgtgtcga gcaagggcat catttatttt accgacccgg
      961 atttccaaaa atcggcagca gccggtggcc agccgcaaac ccgcgcttat cagtatgatg
     1021 gccaaacggt gaaagttatc gaggagaata tgtataaccc caacggcata gcgctgtcgc
     1081 cggatgagtc caccctctat atcgctggca acggcatgtt aaaggcctac ccggttacca
     1141 gcagcggcgt gggcacgggc gtaaacctcg cctctgttac cagcccagac ggtatggcca
     1201 ttgactgctt aggcaatatt tacgtggccg aacacgatca aaaacgctta aaagtcatga
     1261 cgcctaccgg cacacaaatc gcaaccatta atgtggatgc caatgtcacc aacgccgcct
     1321 ttggtggcag caatggtaaa accctctaca tcacaggctc cggcactctg tggaaaattg
     1381 acttaaacgt agtaggtaaa ccctactaag caaaacctag attgttgcaa aagcaaggat
     1441 gcttttgccg cgttcgctat ttcacttccc gcctttttag tctcacctca taactccctc
     1501 gctttatccc ttagcctcct cgtttaacca ttgtcacacg cctttaattc actactggag
     1561 cggtcttttt aacctaaaaa ccgcagttga actcgtagcg agggtgccta aggcgccgaa
     1621 tatgtgcgct tttttatcaa aatttggcga aagcagtagt catctctacg gtgagtcaaa
     1681 ttttgataaa aaagcgtgca gattcaagca ttaggcggcc gtagtagagt tcaactgcgg
     1741 tttttaggtt taacctgtga tgtgttatgg tcgccccacc tgctgattta aaaatgcgtt
     1801 ttactgcctt tagatgggct acaagtaact gacctgcaaa ccctgagcaa aactgtttgt
     1861 agctactgcg cacacctaat tcctcactcc atacagctgg taaattgagc ggagatttag
     1921 cggtgcggct gaatcaattg gcggtgtagc cgtggtgaat taggttattt ccggcctcca
     1981 acaagcaggg ctttttttgc gaaaaaatgt ggcagttact ccaacttcgc tgcggtggtt
     2041 tagctaagcg ttttaagttt tctgatcgct tgaggatatg ccagtggaat ttgcccaaat
     2101 agaaattacc acgcgctgta attttacctg cggattttgc gcgggccgcc atatgcccca
     2161 gcgggatttc agccgcgcgg actttaatgc catcctcgcc cagtggccaa atttaaccgc
     2221 cattgaacta caaggcgaag gcgagccatt aatacatccc gatttttttg cactagtcaa
     2281 cgatgccaaa caccactgcc cagatgctgt gttatccacc atcagcaatg gcagcttatt
     2341 aaatgccagc aatatcgagc agcttttgca atcgccactg gatcacctgt ggatttcctt
     2401 agaaaccctc aatccgctgg attttaagcg tctgcgcggc ggtaaactca gccgagtatt
     2461 gcgcggcctg cgctcactgt tagtcgccaa acgcgctttg gcggctgatt taaaaatagg
     2521 ctttgccgtt actctgctgc acggccatag ccaccaattg caggccatca gccgcttgta
     2581 ccacgcctta ggaatggatg gcggtatcag cctgcagggc ctacaaacca tgcccgctta
     2641 ccagcaacac tacagcgccg aattaattgc cgaattattg cagccccaag ataaccaagt
     2701 aatcaaacaa ttaatcgcca gcgactcgca ggtaaaacgc gccttattcg attatgcccg
     2761 gcagggcagt ttttatgcgc gcatgtacca aaaggccggt gcacactgct gcccatggct
     2821 gacactcggg ctctatattg atgtacaagg gcgcgtaatg agctgctgtt ttagcaaaaa
     2881 cccccaacag gccctcggcc atttcaccca gctcacacag gttctaaagg cgcgcagggc
     2941 catggcccaa tccctggcgg caggcactat tcccagcccc tgcagcggct gcggtatcgc
     3001 ccagcagcta tgcagcgcaa aaaccctcag cgtttaattg ccgctcagta aattctaatt
     3061 ccatcacttt cttactcgct aaccggtgtt aggcttgtca gcgcaaacac acatgggcta
     3121 attgcaatga gaagggatta actcaaaatt gtatttgtag gcaatttgta tttgcgccag
     3181 cggttttgcg gcgcggcttt ttttaatgta gcgcagcttt gatgtaacgc ggctttgatg
     3241 atgcagcact tcgatgccca cccattcata attcattatt atttattaac ccgccgggtt
     3301 taacacccgc cttattcgag cctgccatgc acgcattaac tatttttacg cgcagtgtat
     3361 taattttaag tttaattgcc ctcgccgcct gtagcggccc ggatggcagc gcgagttcct
     3421 ccagcagttc atcaagcagc tcaagctccg gacgcacctc ctcaagtagc agctcctctt
     3481 catcaagcag ttcttcctca tcaagcagtt cttcctcaag ttctggcagc gcaataaacc
     3541 cggtggatgc aggccgcgat caggtggtat ttaacggtga atgggtgcgt ttaaatagca
     3601 gtaatctgcc aaacactaca cttaaatggc gccaattaag cgggccgagc gtaaatctag
     3661 acccgcaacc ggccacaggt tttatcgccc cggatgtgcg ccaaagcact gatttaagct
     3721 ttgaattatc cgcgcaaatt aatgggcaaa cgtttagcga tacggtaaat gtgcgggttg
     3781 agccgtgcaa aaatgccgac ggcatagtgt atggggattg tattagccca ggttttggcc
     3841 ccttcctcgc ctacgagtct gccactggca caggggaagt ttttcactac caaggtacag
     3901 aaaccaaccg ccacgtgcag tggcaaagtg tggatagcgg cgatagcaaa cacggtaagg
     3961 tgatggaaat tcgctggaat gccaacgacc ctaatcacac gattaatgcc aacggctggt
     4021 ttgggcttgc catgcccggc accagcgctg atttacaggc ctataaagac ggcgccatca
     4081 gttttgatat gcgtctcacc taccacgaac agcctaatag tgctgcgcca tttattttta
     4141 aaagcgaatg tattttgccc tgtgtgagcg cggaattaac catcgataag ggcaacagct
     4201 cttttgattg gcaaacccac acctatccag tcgccgattt agttgcctcg ggtttgaacc
     4261 tatccaaggt caaccacctt tgggtaattc aaccagcctg gcgcaaccaa gaacaaaatg
     4321 tcacggtgca aattgataat atccgcctgc taaaaaccta cacgccgcct gtgcctgcca
     4381 atggctgcac cggctctggc aaagtcacct ataccctcag ccgcgcggcc aatccaacgg
     4441 ctgatgagca agaggcctac aatttaatta ccaccgccat ggatttagcg gtaaaaaatt
     4501 acaattgcta caccaattta tcccgccaat tacaaattac ctataacccc agcgtggcaa
     4561 ctgctgatgg cagtaccaat ggcagtattc gctttggctc ccgcgcctcc atgcaccatg
     4621 taaccgccat gcacgaaatt gcccatgtat ttggtgtagg cgccgccaaa tttaaaaccc
     4681 tggtaagcaa cggcatttat accggtccca aagcgacggc gaaattgcgg gaaatttccg
     4741 gcgttgctac ggatcaatta aaatccgacg gcacccactt ttggccccac ggtttaaatt
     4801 atgtcagcga atggaaaaac cagcaggatt taattaatca ctgcagcgtg gtgcaagcca
     4861 tagtggcgga tttagccaac taattccctt ggagtagctt gctattaacc cagcggcaaa
     4921 aaagatcgtc ctgatctatt ttgccttcgc cgcactaaac ctacgaaaat gcgcggattt
     4981 tcttgtttta gtgcggctcg cggcggtttg cacctttgat gcggcatcct tgccgccttt
     5041 attaacccgg cactaattgc cgccaggtta ataacatcag gcaattttag ttaaacacag
     5101 gcgctccact gacttttcag ggcgccttat ttttattcgc tgcagctaaa aaattctttt
     5161 taacctaaaa accgcagttg aactcgtagc gagggtgcct aaggcgccg
//
LOCUS       DAOQGS010000282         5208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8206_length_5208_cov_4.718416, whole genome shotgun sequence.
ACCESSION   DAOQGS010000282 DAOQGS010000000
VERSION     DAOQGS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_5208_cov_4.718416"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKZ52_11075"
     CDS             complement(<1..676)
                     /locus_tag="PKZ52_11075"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNG60358.1"
                     /translation="MKIRNFLALPVVICLSLVLAVLSQHAFSITLSATSNSRSGSITI
                     NISGLASDPNCNSSNTVTLYETKSGTSGLSATTVAGGQLKLYRETGSYSFYATQNRAD
                     FTSVSSPNGATYYCKDRSSPDSTLSVAVSLNRENPAQFDNLRPFVTLNSPPAGQGLVS
                     GTVYPITISAVDTGGSISRVEVRDGTTFLGNAQSSGNSMYTFNWTPTTSSSTPVYKVL
                     TATAFDN"
     gene            1745..2992
                     /locus_tag="PKZ52_11080"
     CDS             1745..2992
                     /locus_tag="PKZ52_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60359.1"
                     /translation="MRIPTLIAAAATALLLAPLSQADVAQVVDLNVVSGEYSITPARC
                     PPNACTITKGTFTGSLTATVVADKAFFSNIKISPNKDGFSLPEDPYTAKQSGATADGK
                     FKFDGKTLDFTGFIDSRAFDGPLVDYHFVAQGAGVVTNEPFNAQGFYLARQDYRKCAS
                     PLCGGIFVKSVNQATTLCADGKRAKECYVAEENYDKLGFDPFSAAPNVGFNTQLLLRG
                     SLAKSKDPIVGKLGVFTATAAYRPANDAEPTGTFYGLEDAGIRCITSPCFSLNEYTLN
                     SKQKRSISDFDLSAVKATDEDVASAYLVLGEGDVLPVAGVNKKVRQLNGTGVKLLANQ
                     FYLPVSAPVAECPEGYSESVKGCVTAGGCVFPQWEQTSVGGAPMKDPITGEIHGVKST
                     ACVDKCPSFATITAPGYCTVNLP"
     gene            complement(3018..3677)
                     /locus_tag="PKZ52_11085"
     CDS             complement(3018..3677)
                     /locus_tag="PKZ52_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60360.1"
                     /translation="MPINPLRHYVLAVLLLVGCTQKPVSEYTSPEVFPTQTHNFQLIK
                     QEIFADPMNGLGLRYQDIAKPGSHFDVNIYPVGGLFWQQMAPMLEGEIAAIMADRDYA
                     ISQGAWQEKREVVRVPVTIAGQAGVRLQWHLWSAQKIQHEIVFLFLQQDKWVKFSFYR
                     TAPSYMAADAVNLAKQAENAVWQLLPELHAPPESPQVAQKRAEHAAKMKAKALELLQA
                     K"
     gene            complement(3677..4486)
                     /locus_tag="PKZ52_11090"
     CDS             complement(3677..4486)
                     /locus_tag="PKZ52_11090"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNG60361.1"
                     /translation="MPLSKFDFHALSTALLPLAQAAGAAILDVIHHNTTLKADASPVT
                     EADLAAHNLLVGALPQVLALPVVSEETPAEDAVLADACWLVDPLDGTKEFIAGNDEFV
                     VCIALMHQCLPIYGFIYQPRTGTAYWGGAQWGSFKCINGGPSEPISARRAHFDEPLIA
                     LVSRQGATQEAPWMEQLSRHWPSGVMQQPMGSALKACVIADGGADIYPRRGRTCIWDT
                     AAAQAILQGAGGDFFNLNGKPLSYNPASCYNPEFIAVADTSLPWLDLLGIA"
     gene            complement(4497..5042)
                     /gene="nudE"
                     /locus_tag="PKZ52_11095"
     CDS             complement(4497..5042)
                     /gene="nudE"
                     /locus_tag="PKZ52_11095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HNG60362.1"
                     /translation="MPTKPQVLKRSRVASSKIFHVEALHMRFANGVEREYERLLSNHH
                     AVIIAPMLDDDRVILAQEYAAGTDDYQWSLPKGKLDPGEDYATAANRELQEEIGYAAG
                     ELILLKNMAQSPNYMQHSTQLVLARNLYPQSAEGDEPEPLNTRIISLSDLESVCQWPD
                     FNEARSIAALFLVRSYLHGRG"
     gene            5088..>5208
                     /locus_tag="PKZ52_11100"
     CDS             5088..>5208
                     /locus_tag="PKZ52_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HNG60363.1"
                     /translation="MIPWQAIDTLLLDMDGTLLDLHYDNYFWLEYLPRAFAAKN"
BASE COUNT         1305 a         1337 c         1303 g         1263 t
ORIGIN      
        1 cattatcgaa agcggttgca gttagaacct tgtaaacagg cgttgagctg ctggttgtgg
       61 gcgtccaatt aaatgtgtac atggagttgc cactagattg ggcattaccc aaaaaggtag
      121 tgccatctcg cacttctact cgagaaatac tgccacctgt gtcaaccgca ctaattgtaa
      181 tagggtaaac ggtgccggaa acgaggcctt ggcctgccgg aggggaattc agcgttacaa
      241 aggggcgaag gttatcaaat tgcgccgggt tttctctgtt tagggaaacg gctaccgaca
      301 atgtagaatc gggtgagctc ctatctttgc agtaataggt agccccgttg ggggaagaaa
      361 cagacgtaaa gtcggcccga ttttgagtag cataaaagct atacgaacct gtctcccgat
      421 agagttttaa ctgccccccc gctacagtag ttgcactaag ccccgatgtc ccgctcttgg
      481 tttcataaag tgtgaccgta ttggaagaat tacaattggg gtcacttgcc aacccactta
      541 tgtttattgt tatcgagcca cttctactat tcgacgtagc acttagagtg atagaaaaag
      601 catgctgact tagaacggcg agaaccagcg agaggcaaat aacgacaggc aatgcaagga
      661 aattacgaat tttcatactt gaaaatctca cacaacaaaa gcaaggcaat acaaatcagc
      721 agagctgcga ctcgactgag ccactccaac aggaagaaga ataatagata tttttaacag
      781 gcggagggtt ggataagcca atcccaggct tgctttttga ttaaaaaccg tggtagagcg
      841 gaaaaactta tcccggttgt aggtgcattt taccgaattg aaaacaaaaa taaagtatga
      901 gcaagacgcc cggcgtaata atcgtgaaat attcgaggtt gattaggcga ttgaccatta
      961 aaggcgcacc acagaaccaa ccggctcgct tacggtgcac tagtttaaac tcgggcgatt
     1021 gctgccaccc ttgcgccaaa gcaatcggaa gaagggcaat ttattttaat ttaattacat
     1081 ttttgtgtaa gccgggcgca tttttacaga cacgcagcaa gattgatgac cacacagatt
     1141 catcgccgcg aattcaatag agccacccaa caattaaatc cattgaaccg gaacaggcgc
     1201 aaaacgagcc aaccactgaa tcaacattga attgcaattt attcatacgg ctttcataaa
     1261 aattaggacg cctccgagaa tcaattttct accgcgttgc acttttttgc atttggtggc
     1321 cgtaatcaat cacaccaatt tcgaacggca acatcctgcg atcaccacga aaattgaatt
     1381 tccgaaactt ccattaatta tcaatacgcc tcttccctta tattcctagc taatttttag
     1441 aaataattta cgcactacca aagcgcctga gccgctcagc gttagccgag ccatccccaa
     1501 attaaattaa gtggttacgc acccgatttt atctatagca ggcggacttt taagcacctc
     1561 gaaaaatctg agatttccat caccctctgg gcgccaacaa aaccgcggca atttccccaa
     1621 tccccaccca aaacaccacc ccactctgca cacttattaa caatttagac acaccgatgc
     1681 tgttaggata gccaggctca gatttgccga gcgcttttgt ttaaccttct cggaaaccta
     1741 aattatgcgt atacctacat taattgctgc tgccgccact gcgctgttac tggcgcccct
     1801 gagccaggcg gatgtggctc aggttgttga tttgaatgtt gtcagcggtg agtattccat
     1861 taccccggcc cgctgcccac ccaatgcctg caccatcact aagggcacct ttactggctc
     1921 cttaaccgct acggtggtgg cggataaagc gtttttctcc aatataaaaa tcagccccaa
     1981 taaagacggc tttagcctgc cggaggaccc ttacacagca aaacaaagcg gcgccacggc
     2041 ggatggtaag tttaagtttg atggcaagac tttggatttc acaggcttta ttgattcgcg
     2101 ggcgtttgac ggccccttgg tggattacca ctttgtggcc cagggcgcag gtgtagttac
     2161 caacgagccg tttaatgcac aggggtttta tttggcgcgg caggattacc gcaagtgcgc
     2221 atcccctctg tgcggcggta tctttgttaa aagcgtgaac caagccacca ccctgtgcgc
     2281 tgatggtaaa cgggctaaag agtgttatgt agcggaagaa aattacgata agctcggctt
     2341 tgacccattc tcggcagcgc ccaatgtggg atttaatacc caattattgc tgcgcggttc
     2401 actggcaaaa agcaaagacc ccatcgttgg caaacttggt gtatttaccg cgactgccgc
     2461 ctaccgcccg gctaacgatg ccgaacctac cggcaccttt tacggcttag aggatgcggg
     2521 cattcgctgc attacatcac cctgtttttc tttaaatgag tacaccctca acagtaagca
     2581 aaaacgcagt atttccgact ttgatctctc cgccgtaaaa gccactgatg aagatgttgc
     2641 cagcgcctat ttagttttag gcgagggcga tgtgctgccc gttgcaggtg tgaataagaa
     2701 ggttcgccaa ttaaacggca cgggcgtaaa actgctggca aatcaatttt atttgcccgt
     2761 cagcgcaccg gttgcagaat gcccagaggg ttatagcgag agcgttaaag gttgcgtgac
     2821 tgcaggcggc tgtgttttcc cccagtggga gcaaacttca gtgggtggcg cccccatgaa
     2881 agacccaatc accggtgaaa ttcacggtgt taaaagcacc gcctgcgttg ataagtgccc
     2941 ttcttttgca accattacag cacctggtta ttgcaccgtt aatctgcctt aggtgatatt
     3001 tttagcgcag ggacgcgcta tttagcttgc aataattcta gcgctttggc tttcattttt
     3061 gcggcgtgtt cggcgcgctt ttgtgccacc tggggggatt cgggtggggc gtgtaattcg
     3121 ggcaataatt gccacaccgc attctccgcc tgcttggcta aattcactgc atcagctgcc
     3181 atatagctcg gtgcggtgcg ataaaaacta aatttcaccc atttatcctg ttgcaaaaat
     3241 aaaaacacaa tttcgtgttg gattttttgc gcgctccaca aatgccactg taagcgcacc
     3301 ccggcttggc cagcgatggt gaccggtaca cgcacaactt cgcgtttttc ctgccatgcg
     3361 ccttggctta tggcgtagtc cctgtccgcc ataatcgccg caatctcgcc ttctaacatc
     3421 ggcgccatct gctgccagaa gaggccgccc accgggtaga tgttcacatc aaaatggctg
     3481 ccgggcttgg ctatatcctg atacctaagg cccaggccgt tcatggggtc ggcaaaaatt
     3541 tcctgtttaa taagttgaaa attgtgcgtt tgggtgggga aaacttcagg gctggtgtat
     3601 tcgctaacgg gtttttgcgt gcagccaact aataataaaa ccgccaacac ataatggcgc
     3661 aaaggattta tgggcattag gcaattccta atagatccag ccagggcagg cttgtgtcgg
     3721 ccacggcgat aaattcgggg ttatagcagc ttgcagggtt gtagcttagg ggtttgccat
     3781 taaggttgaa aaaatccccg cctgcgccct gcagaattgc ttgagccgca gccgtatccc
     3841 aaatacaggt gcggccccgg cgggggtaaa tatcggcgcc gccatcggca attacacagg
     3901 cctttaaagc gctgcccatg ggttgctgca tgacgccgct gggccaatgg cgggagagct
     3961 gctccatcca gggcgcctct tgagtggccc cctggcggct gaccagggca attaagggct
     4021 catcaaaatg ggcccggcgg gcgctgatgg gttcgctggg gccgccattg atgcatttaa
     4081 aactacccca ttgggcgccg ccccaatagg ccgtgccggt gcgaggctgg taaataaacc
     4141 cataaatggg caggcactgg tgcatcagcg caatacacac tacgaactcg tcgttaccgg
     4201 cgataaattc tttggtgcca tctaatgggt ccaccagcca acaggcatct gccaggaccg
     4261 cgtcctcagc cggggtctct tcagacacca caggcagggc taaaacctgc ggcaacgccc
     4321 cgaccagcag attgtgcgcg gccaaatcgg cttcggtaac cggggaggcg tcggctttaa
     4381 gtgtagtgtt gtggtggatt acatccaaaa tagccgcccc agctgcttga gccaaaggca
     4441 aaagggctgt acttaaggcg tgaaaatcga acttagatag cggcattgac tgcgaactaa
     4501 ccccggccgt gcaaataact gcgcactaaa aacagcgcgg caatcgagcg ggcctcgtta
     4561 aagtccggcc actggcagac gctttctaag tcacttaacg aaataatgcg cgtattgagc
     4621 ggctccggct catcgccttc agcgctttgc gggtagaggt tgcgggccaa caccaattgg
     4681 gtgctgtgct gcatgtaatt gggggattga gccatgtttt taagcaggat cagctcgccg
     4741 gcggcataac cgatttcttc ttgtaattcc cggttggcgg cggtggcgta gtcctcgcct
     4801 gggtccagct tgcccttggg cagagaccac tggtaatcgt cggtgccagc ggcatattcc
     4861 tgagccagaa tcacccggtc gtcatccaac atgggggcaa taatgactgc atggtggttg
     4921 gagagcaggc gctcatactc gcgctccacc ccgttggcaa agcgcatatg cagggcttcg
     4981 acgtgaaaaa tcttcgagga ggccacgcgg ctgcgcttta acacttgcgg ttttgtaggc
     5041 atacttaccc cctgttgaat ttttcgtcgc gcggcattat acccacaatg attccctggc
     5101 aggccattga taccttactt ctggatatgg acggcaccct gctggatttg cactacgaca
     5161 attatttttg gctagagtac ctaccccgcg ccttcgctgc taaaaatg
//
LOCUS       DAOQGS010000283         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8257_length_5192_cov_5.309325, whole genome shotgun sequence.
ACCESSION   DAOQGS010000283 DAOQGS010000000
VERSION     DAOQGS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8257_length_5192_cov_5.309325"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /gene="grpE"
                     /locus_tag="PKZ52_11105"
     CDS             <1..503
                     /gene="grpE"
                     /locus_tag="PKZ52_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNG60364.1"
                     /translation="GAAAAAAGPLTFELEAEIERLKDELMQAKEQSLRAMAEAQNIRR
                     RAEQDVEKAHKFGLERFISDLLPVADNLERSIEAAASSSDIKALIEGVELTLKTLQSS
                     LEKQQLLPVNPVGEPFDPQLHQAMTAVPNADCEPNTVLNVFQKGYTLHGRLLRPAMVV
                     VSKKPD"
     gene            688..2634
                     /gene="dnaK"
                     /locus_tag="PKZ52_11110"
     CDS             688..2634
                     /gene="dnaK"
                     /locus_tag="PKZ52_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNG60365.1"
                     /translation="MRPPRPTRRPFSMGKIIGIDLGTTNSCVSILEGNKPKVIENAEG
                     DRTTPSIVAFTQDGEILVGQSAKRQAVTNPNNTLFAVKRLIGRKFTDDVVQKDISMVP
                     YSIVQADNGDAWVEVKGERRAPPQISAEVLKKMKKTAEDYLGEKVTEAVITVPAYFND
                     SQRQATKDAGKIAGLDVKRIINEPTAAALAYGMDKAKGDSTIAVYDLGGGTFDISIIE
                     IADVDGEHQFEVLATNGDTFLGGEDFDMRLIEFFVEDFQKSSGINLKNDPLALQRLKE
                     AAEKAKIELSSSQQTEVNLPYITADASGPKHLAVKLTRAKLESLVEDLVKRSMEPVKT
                     ALKDAGIGVDKINDVILVGGQTRMPLVQKYVADFFGKEPRKDVNPDEAVAIGAAIQGA
                     VLSGDVKDVLLLDVTPLTLGIETMGGVATPLIEKNTTIPTKKSQVFSTAEDNQTAVTI
                     HVVQGERKQAAQNKSLGRFDLSDIPPAQRGMPQIEVTFDIDANGILNVSAKDKATGKQ
                     QSIVIKASSGLSDAEIERMVKDAEANAEADRKFEQLVSARNTLEGLIHATKKTLEEAG
                     DKATADEKSAIQAALSDAEAVVKTDDKEKIEASTQKLTEASSSLAQKLYAEQQAASGG
                     AEKSSGKAEEGAFDAEFEEVKEDK"
     gene            2730..3857
                     /gene="dnaJ"
                     /locus_tag="PKZ52_11115"
     CDS             2730..3857
                     /gene="dnaJ"
                     /locus_tag="PKZ52_11115"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNG60366.1"
                     /translation="MAKRDYYEILGVAKGVSEADLKKAYRRVAMKYHPDRNPDDKDAE
                     EKFKEASEAYEVLSDENKRAAYDQYGHAGLEGAGGMGGGFGGGGFSDIFGDVFGDIFG
                     GGGGGRRGGPARGSDLRYTLELSLEDAVKGTSVKIKVPTLVSCKTCDGSGAKAGTKPA
                     TCTTCGGHGQVRMQQGFFSVQQTCPTCRGKGKIITDPCKDCHGHGRVEETKTLNVKVP
                     PGVDNGDRIRLAGEGEAGAEGGPSGDLYVQISVKEHEFFQRDGKNLYCEVPIGLVEAC
                     LGGEIEVPTLDGRVKLKIPEETQTGKLFRLRGKGVAPVRGGAAGDLLCRVIVETPVNL
                     TAKQKDLLRQLQEDMTGSSTHSPKQSSWFEGMKNFFGDMKL"
     gene            4224..4472
                     /locus_tag="PKZ52_11120"
     CDS             4224..4472
                     /locus_tag="PKZ52_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNG60367.1"
                     /translation="MATLTVTSRGQVTFKKEVLQHLGIKPGEKIELDLLPDGRGILKA
                     ARQTGSIAGFVGVLAGRSNKVASIEEINAAVEQGWASK"
     gene            4472..4867
                     /locus_tag="PKZ52_11125"
     CDS             4472..4867
                     /locus_tag="PKZ52_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNG60368.1"
                     /translation="MRIAVDTNVLVRAAMQDDPVQARIAAQVLNDAELIAVALPCLCE
                     FVWVLLRVYSLQPTEVASAIRALLATANVEVNRPAVESGLAVLETGGDFADGVIAYEG
                     SWLGGETFVSFDKKAIKLLAAQGHSTLQL"
     gene            4934..>5192
                     /locus_tag="PKZ52_11130"
     CDS             4934..>5192
                     /locus_tag="PKZ52_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60369.1"
                     /translation="MKFLANIFLKGFLFTLPLTITFGLIYWLFDALESVLKIPLVLLL
                     PEGQYITGMGVASALVLIFCAGILVQAYVLRQIFAWFDLLLE"
BASE COUNT         1302 a         1339 c         1442 g         1109 t
ORIGIN      
        1 gcggtgcggc tgccgctgcc gcaggcccgc tcacttttga gctggaagcg gaaatcgagc
       61 gcttaaaaga tgaattaatg caggcgaaag agcagtccct gcgggccatg gcggaagcgc
      121 aaaacattcg ccgccgcgcc gaacaggatg tggaaaaggc ccataaattt ggcttagagc
      181 gttttatctc tgatcttctg ccggtggctg acaacttaga gcgctccatt gaagcggccg
      241 ccagcagcag cgatataaaa gcccttattg aaggggtgga gctgacctta aaaaccttgc
      301 aaagcagcct ggaaaaacaa cagttgctgc cggtgaaccc ggtgggcgaa ccattcgacc
      361 cgcaattgca ccaggccatg accgctgtgc ccaacgccga ttgcgaaccc aatacggtgt
      421 taaacgtgtt tcaaaagggc tacaccctgc acggccgcct gttgcgcccg gctatggtag
      481 tggtatcgaa aaagcccgac taagtaccac cgagtttgat cagcccgctg ccgcctttag
      541 ccacaaaaaa tctgtgcaac tgggttgaaa aaactcaagc ggtgccaata ttgatgccaa
      601 gtgcaacaaa acgggcattg ctaacagcca gttaaaaacg attgcaacgc ctggcaactg
      661 caggcaccca aacaacattc aagtgacatt cggccgccac ggcctacgag gagaccattt
      721 agcatgggca aaattattgg tatcgacttg ggcacaacca actcgtgtgt atctattctc
      781 gaaggtaaca aacctaaggt tatcgaaaac gctgagggcg accgcactac gccctccatc
      841 gtagcgttta cgcaagatgg cgaaatcctg gtgggccaga gcgcaaagcg ccaggcggta
      901 accaacccca acaacacctt attcgccgtg aagcgcctga tcggccgcaa attcaccgac
      961 gatgtggtgc aaaaagatat ttccatggtg ccgtacagca tcgtgcaagc cgacaacggc
     1021 gatgcctggg tggaagtgaa aggcgagcgc cgcgcgcccc cgcagatctc cgctgaagta
     1081 ttgaaaaaaa tgaaaaaaac cgccgaggat tacctgggcg agaaagtcac cgaagcggtc
     1141 attactgtgc cggcctattt caacgactcc cagcgtcagg ccaccaaaga cgccggtaaa
     1201 atcgccggtt tagatgttaa acgcatcatc aacgagccca ccgctgcggc cctggcctat
     1261 ggtatggaca aagccaaagg cgacagcacc atcgcggtgt acgacctagg tggtggtacc
     1321 tttgatattt ctatcatcga aatcgccgat gtagacggcg aacaccaatt tgaagtattg
     1381 gccaccaacg gtgacacctt cttaggtggt gaagactttg atatgcgttt gattgagttc
     1441 ttcgttgaag acttccaaaa atccagcggt attaacctga agaacgatcc cttggctctg
     1501 cagcgcttaa aagaagcggc ggaaaaagcc aaaatcgaat tgtcttccag ccaacaaact
     1561 gaagtgaacc tgccgtacat cacagccgat gcctccggcc ccaaacactt ggcggtaaaa
     1621 ctcacccgcg ccaagttgga gtcactggtt gaagacctgg ttaagcgctc tatggagccg
     1681 gtcaaaactg ctcttaaaga tgccggtatc ggcgtggata aaatcaacga tgtgattttg
     1741 gtgggcggtc aaacccgtat gccactggtg caaaaatacg tggcggattt cttcggtaaa
     1801 gagccgcgca aagacgtcaa cccagacgaa gctgtggcca ttggtgctgc cattcaggga
     1861 gcggtgctct ccggtgatgt taaagacgta ctgttgctgg acgtaacccc gctgaccttg
     1921 ggcattgaga ccatgggcgg cgtggcaact ccgttgatcg agaaaaacac cacgattcca
     1981 accaagaaat cccaagtctt ctccacggcg gaagataacc aaacggcggt taccatccac
     2041 gtggtacaag gtgagcgcaa acaggcggca caaaacaaat ccttgggccg cttcgacctc
     2101 tccgatatac caccggccca gcgcggtatg ccgcaaattg aagtgacttt tgatattgac
     2161 gccaacggta ttttaaacgt gagcgccaaa gacaaggcca ccggtaagca gcagtccatt
     2221 gtgattaaag ccagctccgg cttgagcgat gcggaaatcg aacgcatggt gaaagacgcc
     2281 gaagccaacg ctgaggcgga ccgcaagttt gagcagctgg tcagcgcgcg caacaccctg
     2341 gaaggcctga ttcatgctac gaagaaaacc ttggaagagg cgggtgataa agcgactgca
     2401 gatgaaaaat cagccatcca agcagccctc agcgatgccg aagctgtcgt taaaacggac
     2461 gataaagaaa aaattgaagc ctcgacacag aagctgaccg aagcgtcttc aagccttgcc
     2521 caaaagctct acgctgagca gcaagctgct tccggcggcg cggagaaatc cagcggcaaa
     2581 gctgaagaag gcgcttttga cgctgagttt gaagaggtga aagaagataa gtaatcccgc
     2641 tgcggccttg cgccgcagcg tttttttgga tttgggttaa gcagggctaa gctctgcttt
     2701 gttgtttctg accacggaaa aagcgttaca tggctaaacg tgactattac gaaattttag
     2761 gggttgccaa gggcgtttcc gaagcggact tgaaaaaggc ctaccggcgg gtggccatga
     2821 aataccaccc ggaccgcaac ccggacgata aagacgccga agagaaattc aaagaggcca
     2881 gcgaagccta tgaagtgctc tccgacgaga acaaacgcgc cgcctacgat caatacggcc
     2941 acgcgggcct tgaaggcgcc ggcggtatgg gcggcggctt cggtggcggc ggcttcagcg
     3001 atattttcgg cgatgtgttc ggcgatattt tcggcggagg cggcggtggc cgcagaggcg
     3061 gcccagcccg tggctccgac ctgcgctaca ccctggagtt aagcctggaa gatgccgtaa
     3121 aaggcaccag cgttaaaatc aaagtaccca ccttggtgag ctgcaaaacc tgtgatggca
     3181 gcggcgccaa agcgggcacc aagcctgcca cctgtaccac ctgtggcggc cacggccagg
     3241 tgcgcatgca acagggcttt ttctcggtgc aacaaacctg tccgacctgt cgcggcaagg
     3301 gcaaaatcat caccgacccc tgtaaagact gccacggcca cggccgggtg gaagaaacca
     3361 aaacccttaa cgtcaaggtg ccaccgggcg tggacaacgg cgatcgcatc cgcctggcgg
     3421 gcgagggtga agccggtgcc gagggcggcc catcgggcga tctgtacgtg cagatttccg
     3481 ttaaagaaca cgagttcttc cagcgcgatg gcaaaaatct ctactgcgaa gtacccattg
     3541 gtttggtgga agcctgcctc ggcggcgaga ttgaagttcc gactttggat ggccgggtga
     3601 agttaaaaat ccccgaagag acccaaaccg gcaaactgtt ccgcctgcgc ggcaagggcg
     3661 tagctcctgt acgcggcggt gccgccgggg atttactctg ccgcgtgatt gtggaaacac
     3721 cggttaattt aactgccaaa caaaaagatc tgctgcgcca attgcaagag gatatgaccg
     3781 gcagctccac ccactcgcca aagcaatcca gttggtttga ggggatgaag aacttttttg
     3841 gggatatgaa gttgtaaccc ttcggtagtt aaaagaaagg ccagctttgc tggcctttct
     3901 ttttgcacac ttgtagaagc gttatgaatt ttttggacaa taatttttta agaggctttt
     3961 tgtttatcct gccgctgatg atcattttcg ggctcatcta ttggctgttt ggcgcctcaa
     4021 cgcgtaatcc agatcagtag ggcgcacctt ggtgcgccac accagatact ccggcttatt
     4081 aaattaaaag cggcgcacca aggtgcgccc tacagggctc catccagatc cgtcgtgatc
     4141 cccatcccgc caccgctctg gcatttgacg gtaaggtatt tttgcattac tattcggttt
     4201 ctgatcaaaa cgacgagggc gctatggcta cgttgaccgt cacctcaagg gggcaagtca
     4261 cctttaaaaa agaggtactg cagcatctgg gcattaagcc gggggagaaa attgagttag
     4321 atttattacc tgatggtcgc ggtattttga aagccgcccg gcaaaccgga agtattgccg
     4381 gtttcgtggg agtgctggcg ggccgctcga acaaagtcgc cagcattgag gaaatcaatg
     4441 ccgccgtcga gcagggttgg gccagtaagt aatgcgtatt gcggttgata ccaatgtact
     4501 ggtgcgtgcc gctatgcaag acgacccagt gcaggctcgt attgccgccc aggtgttgaa
     4561 tgatgcggag ttaattgcgg tggcgctgcc gtgtttgtgt gaatttgtat gggttttgct
     4621 ccgggtttat agcttgcaac ctaccgaagt cgcgtccgct attcgcgcgt tattggctac
     4681 ggcgaatgtg gaggtcaata ggcctgcggt ggagtctggc cttgcggttc ttgagacagg
     4741 tggggatttt gccgacggtg taattgccta tgaaggtagt tggttgggcg gtgaaacctt
     4801 tgtttccttc gataaaaaag ctatcaaatt gctggccgct cagggccatt cgacgctgca
     4861 gttgtaaaac ccaacacgct gttaaatcct ccacttccct ctgttagaga gggagattac
     4921 cgccaggcgc accatgaaat tcttagccaa tatcttccta aaaggctttt tatttaccct
     4981 gccgctcaca atcacctttg ggttgattta ttggctgttc gacgcacttg agtcggtgtt
     5041 aaaaatcccg ctggtgctgt tattacccga gggacaatac attaccggca tgggggtggc
     5101 ttcggctctg gtgttgattt tctgcgcggg gattttggtg caggcctatg tactgcggca
     5161 gatttttgcc tggtttgacc tgttgctcga gc
//
LOCUS       DAOQGS010000284         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8330_length_5160_cov_4.806072, whole genome shotgun sequence.
ACCESSION   DAOQGS010000284 DAOQGS010000000
VERSION     DAOQGS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8330_length_5160_cov_4.806072"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..2030
                     /locus_tag="PKZ52_11135"
     CDS             579..2030
                     /locus_tag="PKZ52_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNG60370.1"
                     /translation="MTINWQGYKPADFYDELIEKPGKPRQSAEHLVAYLQSLGDEEIE
                     RRREAAEATVREMGVTFTVYTDEGNIDRAWPFDIIPRTISKKQWDVTAEGLKQRLQAL
                     NMFITDLYHEQKIVRDGVLPRYILEQSKNFRPECVGIMPRYGVWANICGTDLVRDKDG
                     EFYVLEDNLRVPSGVAYMLENRAITKRVLPDLFEQAQIAPIHDYPARLFDMLVSLSPR
                     DIEQPVVVVLTPGIFNSAYFEHAFLAQQMGAELVEGSDLVVQADDCVYMKTVSGLERV
                     DVIYRRIDDLFLDPEAFIPDSILGVPGLMRAWKKGNVGLANAPGAGVADDKVVYAFVP
                     KIIKYYLDQDPIVPNVETFLCVNPKEMEYVLANLEKLVVKPANESGGYGMIVGPHSTK
                     KERTEFAERIKADPRNYIAQPTLALSTAPTLVGNNSLEPRHLDLRPFILQGASTYVTT
                     GGLTRVAMKKGSLVVNSSQGGGSKDTWVVDTGA"
     gene            2035..2961
                     /locus_tag="PKZ52_11140"
     CDS             2035..2961
                     /locus_tag="PKZ52_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNG60371.1"
                     /translation="MLSKVAERIYWTARYIERVENTARLLGVYANLLLDLPKGINLGW
                     LNLIELNSAQAQFDSNYKTYDERNVVKFILGDESNPSSMVSSLRMLRENVRTSRDVVP
                     GETWELVNALSMFVQENLSQAIKRGQRHAFLKGVIRGCQQINGLMYGTMSHDKAWDFL
                     RLGRNLERADMTTRILDIGVATWMHAQHNDGASYAKQIIWGNVLRSVGAEQSYRRSLR
                     AAVDAADVAQYLLEDEQYPRTILHCLHAMDDSACKLPLAEPVVLALANLQTNLFANVN
                     YDVLDDSFREYLNNMQLELAKLHTSIADNWFN"
     gene            3086..>5160
                     /locus_tag="PKZ52_11145"
     CDS             3086..>5160
                     /locus_tag="PKZ52_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNG60372.1"
                     /translation="MTIRVAINHQTHYDFDREVGVSPHTVRLRPAPHCRTPIHAYSLK
                     IQPEEHFINWQQDAFGNFLARLVFPEKTKRFWVDVEVIADMTVINPFDFFLEEYAENF
                     PFKYDEQTHKELLPYLECLECGSQFDAVLATLPKTKMRTIDFLVAVNQKVQSLVEYTV
                     RLEPGVQTPEETLEKALGSCRDSAWLLVQLLRHVGLAARFASGYLVQLKADVASLDGP
                     SGPEADFTDLHAWCEVFIPGAGWVGLDPTSGLFASEGHIPLACTPDPVSAAPIHGFTD
                     KCQVEFNFSNLVTRIHEDPRVTKPYTEGQWDAVMALGQKVDELVAAQDVLLTMGGEPT
                     FVSIDDMESAQWNTEALGEHKLTLAKDLLLKLRDSFAPTGLLHYGQGKWYPGEEVPRW
                     ALGIFWRKDGEPLWQEQEYLARVDKNYGFGLREAHLFMLQICSRLALDAEFCQAAYED
                     GLHYLMQEQNLPVNLDSQIADAKDELARRRLAKVLERGIAKPTGYVLPLAFNGGMGRW
                     VSSRWPTRRERIVLIPGDSPMGLRLPLNSLPFVEESLREELIPVDPFAPHDPLPKHSD
                     IPSRYIAKTVAAEPQVQAQVIEPQGPKWERRVHKEYWDVLRTAMCVEARDGRLHIFMP
                     PLNYLEHYVQLLACIEATAKALKLPVIIEGYEPPRDSRLLKLLVTPDPGVIEVNIHPA
                     SSWNELVHTT"
BASE COUNT         1174 a         1257 c         1445 g         1284 t
ORIGIN      
        1 aaggcttggc ttgcacaggc caataccaga gctgcaaccc aaagtcccca aaggtgcttt
       61 tgcttggtta cataactgtg tgctctggcc atccactttg ctccgctagc aggctggtta
      121 atgacgacgg caatctacac gtcgttcttt aaagttagca cagggttgcc gcttgtaaac
      181 tcagacgcag cccgtgacat tttttaaacg attgggtttt aagccgtgca cgcaggtcct
      241 ggcatgaaac ctgttagccc tagcggtaaa agggggatta gttgcggctg agccgagcgc
      301 ttaattttct ggtgatgccg tggtgtgaac ctgcaagagg gtgtgcgact tggaaggggt
      361 tgtgcatagc agcttgcgtc atgaactcgt tggtgtgccg ccagcgggag aacataaaaa
      421 gggggtgagc cttaaacaac tgtgctaggg gttggtgaag gcgttagcgt atcgactagg
      481 tattaatcat ttcctgcgta tgtgtggcac ctgcaggcgc tgtgcacgcg ttgggaaatc
      541 actgtaaaaa gcccaataaa taatgatggt aattcgttat gacgattaac tggcaaggct
      601 acaaacctgc agatttttac gatgaattga tagaaaagcc gggcaaacct aggcagtcgg
      661 cggagcattt ggtggcttat ttgcaatccc tgggggatga agaaattgag cgccgccggg
      721 aggccgctga agcaaccgtg cgggaaatgg gcgtcacctt cactgtatac accgatgaag
      781 gcaatatcga ccgcgcctgg ccattcgata ttatcccgcg caccatctcc aaaaagcagt
      841 gggatgttac cgctgagggt ttaaagcagc gcttgcaagc cctgaatatg tttattaccg
      901 acctatacca cgagcaaaaa attgtgcgcg atggtgtttt accgcgctac atcctcgaac
      961 aatcgaagaa cttccgccct gagtgcgtag ggatcatgcc tcgttatggc gtttgggcca
     1021 atatttgcgg cactgatttg gtgcgcgaca aggatggcga gttttatgtg ttggaggaca
     1081 acctgcgggt gccctcaggt gtggcttata tgctggagaa ccgcgccatc accaagcggg
     1141 tgttgcccga ccttttcgag caggcccaaa tcgcccccat tcacgattac cccgcgcgcc
     1201 tgttcgacat gctggtgtcc ctgtcgccgc gtgacataga gcagccggtg gtggtggtat
     1261 taacaccggg catttttaat tcggcctatt ttgaacacgc ctttttggcg cagcaaatgg
     1321 gtgcggagct ggtggaaggc agcgacttgg tggtgcaggc agacgactgt gtttatatga
     1381 agacagtgtc tggccttgag cgcgtggacg ttatttaccg ccgtatcgac gatttatttt
     1441 tagaccctga agcctttatc ccagattcga ttttgggtgt gcccggttta atgcgggcgt
     1501 ggaaaaaggg caacgtgggc cttgctaatg ccccgggcgc cggggtggcg gatgataagg
     1561 tggtgtacgc ctttgtgccg aagattatta aatattattt ggatcaagat ccgattgttc
     1621 ccaatgtgga aacctttctg tgtgtaaatc ccaaggagat ggaatatgtg ctggcgaatt
     1681 tggaaaaact ggtagtgaag cctgcgaatg aatctggcgg ctacggcatg attgtagggc
     1741 cgcactccac caaaaaagag cgcactgaat ttgccgagcg cataaaagcg gacccgcgca
     1801 attatattgc tcagcctacg cttgcgcttt ccactgcacc tactttagtg ggtaataaca
     1861 gcctggagcc gcgtcacttg gatttacgtc cgtttattct gcaaggtgca tctacctatg
     1921 tcactacggg tggtttgacc cgtgtagcca tgaaaaaagg ctcgttggtt gtgaactcgt
     1981 cgcaaggcgg cggaagtaaa gacacttggg ttgtggatac cggagcttaa tattatgttg
     2041 tcaaaagtag ctgaacgaat ttattggacg gcgcgttata ttgagcgtgt ggaaaataca
     2101 gcgcgtttat tgggtgtgta cgcgaattta ttattggatt tgcccaaagg cattaattta
     2161 ggttggctga atttgattga gcttaacagt gcacaagcgc aatttgattc aaattataaa
     2221 acctatgatg agcgcaacgt ggtgaaattt attctcggcg acgagagcaa ccccagctct
     2281 atggtgtcct cactgcggat gctgcgggaa aatgtgcgca cctcaaggga tgtggtgccg
     2341 ggtgaaacct gggagctggt gaatgctctg tcgatgtttg tgcaggaaaa tctttcccaa
     2401 gccataaaac gcggccagcg gcatgcattt ttaaagggtg ttattcgcgg ttgccaacaa
     2461 attaatggct taatgtacgg caccatgagc cacgataaag cctgggattt tttgcgctta
     2521 gggcgtaacc tggagcgcgc tgacatgacc acgcgcattc ttgacatagg tgtagctacc
     2581 tggatgcacg cgcaacacaa cgacggcgcc tcctacgcca aacaaattat ctggggcaat
     2641 gtgctgcgct cggtgggtgc cgagcaaagt tatcgccgca gtttgcgcgc cgctgtggac
     2701 gctgcggacg tggcgcagta tttgttggaa gatgagcagt acccgcgcac gattttgcat
     2761 tgtttacacg ccatggacga ttctgcgtgt aaactgcctt tggccgagcc ggtggtgttg
     2821 gccttagcta atttgcaaac gaatttattt gccaacgtca attacgatgt gttggatgat
     2881 tcgtttcgtg aatatttaaa taatatgcag ttggagcttg ccaagctgca cacctctatc
     2941 gccgataatt ggtttaatta attggctgta taaataaggc tgattaattg tgttaatcag
     3001 cccagaacct tcgctgtggg cgtgtattta tcgctcctga atacaatcta tgcgtggtta
     3061 gccatgtgtt tcactggacg taattatgac gattcgagtc gctattaatc atcaaacaca
     3121 ctatgatttt gaccgcgaag ttggcgtatc gccgcacact gtccgtttgc ggcccgcacc
     3181 gcactgccgc acgcccattc acgcctatag tttaaaaatt cagccggaag aacactttat
     3241 taattggcag caggatgcct ttggcaattt cctcgcccgc ttggtatttc cggaaaaaac
     3301 caaacgcttt tgggtagacg tggaagtgat cgccgatatg acggtgatta atccctttga
     3361 ttttttcttg gaagaatacg ccgagaattt tccgtttaaa tacgacgagc aaacccacaa
     3421 agagcttttg ccctatcttg aatgcctcga atgcggcagc caatttgacg ccgtgctggc
     3481 aacactgcct aaaaccaaaa tgcgcaccat tgattttttg gtggcggtga atcaaaaggt
     3541 gcaatccctg gtggaatata cggtgcgctt agagccgggc gtacaaaccc ctgaggaaac
     3601 ccttgaaaag gccctgggtt cctgccgaga ttccgcctgg ttattagtgc aattattgcg
     3661 gcatgttggc ttggcggcgc ggtttgcctc tggctatttg gtgcaattaa aagctgatgt
     3721 ggcaagcctg gatggcccct ccgggccaga ggcggatttc acggatctgc acgcctggtg
     3781 tgaggtgttt attcccggcg ccggttgggt ggggcttgac cccacctccg gcctttttgc
     3841 cagcgaaggc catatccctc tcgcctgtac gcccgaccca gtctccgcag cgcccatcca
     3901 tggctttacc gataaatgtc aggtggaatt taacttctcc aacctggtga cgcgcatcca
     3961 tgaagacccg cgcgtcacca agccctatac cgaaggccaa tgggatgctg tgatggccct
     4021 ggggcaaaaa gttgatgagc tggtggcagc ccaggatgtg ctcctcacta tgggcggcga
     4081 gccgaccttt gtgtccattg acgatatgga atcggctcag tggaatacgg aggccctcgg
     4141 tgagcacaaa ttaaccctcg ccaaagacct actcttaaag ctgcgcgaca gttttgcccc
     4201 cacggggctg ctgcattacg gccagggcaa atggtatcca ggggaggagg tgccgcgctg
     4261 ggcgctgggt attttctggc gcaaagatgg cgagcccctt tggcaggagc aagaatactt
     4321 agcgcgggtg gataaaaatt acggttttgg cctgcgcgag gctcatctat ttatgctgca
     4381 aatctgcagc cgcttggccc tggatgcgga gttctgccag gccgcctatg aagatggcct
     4441 gcattatttg atgcaagagc agaatctgcc ggtgaatttg gattcacaaa ttgccgatgc
     4501 caaggacgag ctggcccgcc gtcgcctggc taaggtgtta gagcggggaa tcgccaagcc
     4561 caccgggtat gtgttgccgc tggcctttaa tggcggcatg ggccgctggg tgagcagccg
     4621 ctggcccacc cggcgggagc gcatcgtatt gatccccggc gattccccca tgggtttgcg
     4681 cctgccgctc aattccctgc cttttgttga ggagtccctg cgggaagaat taatccccgt
     4741 cgatcccttt gcaccccacg acccgctgcc caaacacagt gatattccca gccgttacat
     4801 tgccaaaacg gttgccgccg agccgcaggt gcaggcccag gttattgagc ctcaaggccc
     4861 taagtgggag cgcagggttc ataaagagta ttgggatgtg ctgcgcaccg ccatgtgtgt
     4921 ggaggcccgc gatgggcgcc tgcatatatt catgccgccg ttaaattatc ttgaacacta
     4981 tgtgcagttg ctcgcctgta tcgaagccac cgccaaagcg ctcaagctgc cggtgattat
     5041 tgaaggctac gagccgcccc gagacagccg cctgcttaaa ctgttggtca ccccagaccc
     5101 aggtgtcatt gaagtgaata tccacccggc cagcagttgg aatgagctgg tgcataccac
//
LOCUS       DAOQGS010000285         5153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8346_length_5153_cov_3.740879, whole genome shotgun sequence.
ACCESSION   DAOQGS010000285 DAOQGS010000000
VERSION     DAOQGS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_5153_cov_3.740879"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="epsF"
                     /locus_tag="PKZ52_11150"
     CDS             complement(<1..1104)
                     /gene="epsF"
                     /locus_tag="PKZ52_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HNG60373.1"
                     /translation="MSIQQFLLIFFARKGPALAVFAVTVLTAIGLSIILPKTYQATNT
                     VVIDLKSPDPIAGVVLPGMMTAGYMATQVDIVNSDRVAQRAVGLLKLEENPAVRDQWQ
                     QETQGRVSLPVWMGNLLQKNLDVKPSRESSVVNITFSAPDPTYAAAAANAFAQAYIDT
                     TVQLKVDPAKQYASWFETQIKSQRDRLDAAQKALADFQEQSGIVISDGRLDYELQKFN
                     ELSTQLTMAQAQGTDSTSKQLLTQVDTLPEVMQNPLINQLKAELVRMESSLKDLSGNL
                     GVNHPAYKRALSEQQSLKNRIADEITHVTSAIGTAGLVSQAKVAELTTAIEEQKKKVF
                     ELRRQRDTINLLMRDVETAQRAYDGISQRYNQIA"
     gene            complement(1114..1917)
                     /gene="epsE"
                     /locus_tag="PKZ52_11155"
     CDS             complement(1114..1917)
                     /gene="epsE"
                     /locus_tag="PKZ52_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HNG60374.1"
                     /translation="MRFCAHWLVLLACLFQLPAVAADKPNLSYRLGSGDVIRITVFQN
                     PDLALETRVSESGTITYPLIGSVSLGGLTLEAAEKLIAKGLKDGGYVQAPQVNIGLTQ
                     VRGSQVSVLGQVNRPGRFPLETFNTRLSDVLAMAGGIAVTGSDKVVITGTREGKSFRQ
                     EVDFPAIFSGINNQQDMLIAGGDVVYVNRAPVFYIYGEAQRPGAYRIEREMTVQQALA
                     IGGGATQRGSDSRIRINRKGPDGKTKQISPELSDLVLPDDVIYIRESIL"
     gene            complement(1940..2827)
                     /locus_tag="PKZ52_11160"
     CDS             complement(1940..2827)
                     /locus_tag="PKZ52_11160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNG60375.1"
                     /translation="MAAIPAKPTQLFSALLCALLALGSSGCGRDDKPKTTQVAAKVNG
                     EEISVHQINSLLAKTPNLTPEAAEKVRSEVLEKLIDQELMYAQARSKKLDRTPEVVAA
                     IEAAKREIIARAHLEQLLSTLPKVTEQEVKDYYNNNKPLFAERRIYNLEELSVEANPE
                     ILPKLKEMVDSKSLDELATALTALGAPARKGAATRAAEQIGLDLLPQLAQVKDGDKAL
                     IIGSKNYLVVHVLSSKSEPVNAEMAAPKIAQFLQTQRVQKAVADETKRLRGAAIVEYQ
                     GDFAKAPTTAAPAATPDAK"
     gene            complement(2919..4166)
                     /gene="epsL"
                     /locus_tag="PKZ52_11165"
     CDS             complement(2919..4166)
                     /gene="epsL"
                     /locus_tag="PKZ52_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative exosortase B-associated extracellular
                     polysaccharide biosynthesis transporter EpsL"
                     /protein_id="HNG60376.1"
                     /translation="MSVGFAHFQSSLRRCVFALCALAASFSQAQEQYGLGWQIGSQLE
                     HEENVFLKPANQANSDSVLGSFAAVDYRKRYSLQTFIIKAKVTDFRYKRFDYLNYLGK
                     DLDASWQWAFTPSLTGALSSSRQEGLNSFIDYDGSKRNLRKERRDALDVRWRITGGWH
                     LAAGGDQSAVRNTLGGERFAEGDYTANNVEYGLMYSFPSQSQITLVQMNQSGEYRSRT
                     LSELSQFDSGFDQNGQELRLDWRLSGKSNLNLKLGHLTREHDHFPRRDYSGTYGSADY
                     LWYNGGKLKIKLAAKQNYSSFQSSPQRDFYDASLGRDYYNSSYYRSQLLSITPSWQLL
                     DKIALSGRVQQEEREYLGGLLSGPQLRQRQDTLRQGTVSLDWVPRDVVALSLHMSRQE
                     RNSNMPSAEFTADSIGISAALSF"
     gene            complement(4345..>5153)
                     /locus_tag="PKZ52_11170"
     CDS             complement(4345..>5153)
                     /locus_tag="PKZ52_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNG60377.1"
                     /translation="CHWVWGGGWLMSMGMLDFAGGSVVHITAGVAALVAALVIGNRHG
                     FPNTAMPPHNMTMTVTGAGMLWVGWFGFNGGSALMANGNASMAMLVTHLSAAAGALAW
                     MGMEWWRFSKPSVLGAVTGMVAGLGTITPASGSVGPAAALLIGLLAGVICFYATHWIK
                     RTLHIDDSLDVFPVHGVGGFLGTFLVGIFASTDLGVFSGFGFGAGNESIWAQVKVQLI
                     GMLAVGGYTAAATWGILKVTGLCTNGIRVSPEDESQGLDISLHEEAGYKL"
BASE COUNT          993 a         1378 c         1577 g         1205 t
ORIGIN      
        1 ggcgatttgg ttgtagcgct ggctgatacc gtcgtaggcg cgctgggcgg tttctacatc
       61 gcgcattaat aggttaattg tgtcccgctg gcgacgcagt tcaaagacct ttttcttttg
      121 ctcttcgatg gcggtggtga gttctgccac cttggcttgg ctgaccaaac ctgcggtgcc
      181 tatggcgctg gtgacatggg taatttcatc agcgatgcga tttttaaggg actgctgctc
      241 agacagagct cgcttatagg cggggtggtt aacacccaaa ttgccggaga ggtctttaag
      301 gctcgattcc atgcgcacca actccgcttt gagctggtta atcagcgggt tttgcattac
      361 ttccggcagg gtgtccacct gggttaacag ctgtttactg gtggaatccg ttccctgagc
      421 ctgggccatg gtgagctggg tggacagctc gttgaatttt tgcagttcgt aatcgagcct
      481 gccgtcggag atgacgatgc cggactgctc ttgaaaatcc gccagggctt tttgggcggc
      541 gtccaggcgg tcgcgctggg atttaatttg ggtttcaaac cagctggcgt attgcttggc
      601 cgggtccact tttaattgca cagtggtgtc gatataggcc tgggcgaagg cattggcggc
      661 ggctgcggca taggttgggt cgggcgcgct gaaggtgatg ttcaccacac tgctctcacg
      721 ggagggtttt acgtcgaggt ttttttgcag caggttgccc atccatacgg gcaggctcac
      781 gcggccttgg gtctcctgct gccattgatc gcgcacggcg gggttttctt ccagctttaa
      841 taggcccacg gcccgctggg ctacgcggtc actgttcact atgtccacct gggtggccat
      901 atagcctgcg gtcatcatgc cgggcagtac cacaccggca attgggtccg ggcttttcag
      961 gtcgatgacc accgtattag tggcttgata ggttttgggc agaatgatgc taaggccaat
     1021 ggccgtcagc accgtcaccg caaataccgc tagggccggg cctttgcggg cgaaaaaaat
     1081 caataaaaac tgctgaatac tcatgccagc cccttacaga atgctttcgc ggatgtagat
     1141 aacgtcgtcg ggcagaacca ggtcggacag ctccggggag atctgcttgg ttttgccgtc
     1201 tgggcctttg cggttgatgc gaatgcggct gtcgctgccg cgctgggtgg cgccgccgcc
     1261 gatggcgagg gcctgctgta ccgtcatctc ccgctcaatg cggtaggcgc cggggcgctg
     1321 ggcttcgcca tagatgtaaa acaccggggc gcggttcacg tagaccacat cgcccccggc
     1381 gataagcatg tcctgctggt tgttgatgcc gctgaagatg gctgggaagt ccacttcctg
     1441 gcgaaagctt ttgccctcgc gggtgccggt gatgacgact ttatcggagc cggtaacggc
     1501 gatgccgccc gccatggcga gcacatcaga aagccgggtg ttaaaggttt ccagcgggaa
     1561 gcggccgggg cgattgacct ggccaagtac ggagacttgg ctgccgcgca cctgggtaag
     1621 gccgatgttc acttggggcg cttgtacgta gccgccatct tttaagcctt tggcgatgag
     1681 tttttccgcc gcttccaggg tgaggccgcc aaggcttacc gagccgatta agggataggt
     1741 gatggtgccg gattcggaca cgcgggtttc tagggcgagg tccgggtttt ggaatacggt
     1801 gatgcggata acatcgccgg aacctaggcg gtagctgagg ttgggcttgt ccgccgcgac
     1861 ggcgggcaac tgaaataaac aggccaagag caccagccag tgagcgcaaa atcgcatgga
     1921 atcttctcct gaagttgggt tacttggcgt cgggggtagc ggcaggcgca gcagttgtgg
     1981 gcgctttggc gaagtcgcct tggtattcca ctatggcggc gcctcttagg cgtttggttt
     2041 catcggccac ggctttttgt acccgctggg tttgtaaaaa ttgggcgatt ttaggtgcgg
     2101 ccatttccgc attcaccggt tcactcttgg aggaaagtac gtgtaccacc aggtaattct
     2161 tgctgccgat aatcagcgct ttatcgccgt cttttacctg ggctaactgg ggcaataaat
     2221 ccaggccgat ctgctcggcg gcgcgggtgg cggcgccttt gcgggcgggg gcgcccaggg
     2281 cggttagggc tgtggcaagt tcatccaggc ttttgctgtc taccatctct ttaagcttgg
     2341 gcagaatctc ggggttggcc tccaccgaga gttcttccag gttgtaaatg cggcgctcgg
     2401 cgaacagcgg tttattgttg ttgtaatagt ctttcacctc ctgctcagtc accttcggca
     2461 gggtgctcag cagctgctct aagtgggcgc gggcgatgat ttcgcgcttg gccgcttcaa
     2521 tggcggcgac gacttccggg gtgcggtcga gttttttgct gcgggcttgg gcgtacatca
     2581 attcttggtc gatgagtttt tccagcactt cgctgcgcac cttttcggcg gcttccgggg
     2641 tgaggttggg ggttttggcc agcaggctat tgatttgatg aacagagatt tcctcgccgt
     2701 tcaccttagc ggccacctgg gtggttttgg gtttgtcgtc ccgcccgcag ccgctgctgc
     2761 caagggctaa gagcgcgcat aacagggcgc tgaataattg cgtgggtttg gctggaatgg
     2821 ccgccatagt attcccctta ttgattttat ctgcattggc ggtttgggcg gcctggccgc
     2881 ttcaccggtt ttgttatggg ttggctaccc tggtcttttt aaaagcttaa cgctgcgctg
     2941 atgccgatgg agtcggcggt aaattctgct gagggcatat tggagttgcg ctcttggcgg
     3001 ctcatatgca gcgacagtgc cacaacatcc ctaggcaccc agtcgaggga gacagtgccc
     3061 tggcgcaaag tatcctgccg ctggcgcaat tgggggccgc ttaacaggcc gccgagatat
     3121 tcccgttcct cctgctgcac gcgcccgctt aaggcaattt tgtcgagcag ctgccaggaa
     3181 ggggtaattg agagcagctg gctgcggtaa taactgctgt tgtaatagtc ccgcccgagg
     3241 ctggcatcat aaaaatcccg ctgggggctg gattgaaacg agctgtaatt ctgcttggcg
     3301 gcgagtttta tttttaattt gccgccgttg taccacaggt aatccgccga gccataagtg
     3361 ccactgtagt cccgccgggg gaagtggtcg tgctcccgcg ttaaatggcc gagcttgagg
     3421 ttgaggttgg atttaccgga caggcgccaa tccaagcgca gctcttggcc gttctggtca
     3481 aaaccgctgt cgaactggct caactcactc agggtgcggc tgcggtattc gccgctttgg
     3541 ttcatctgca ccagggtaat ttggctctgg gaggggaagc tgtacatcag gccgtattca
     3601 acattgttgg cggtgtaatc cccttcggca aagcgctcgc cgcccagggt gttgcgcacg
     3661 gcgctctggt cgccgcctgc ggccaagtgc cagccgccag taatgcgcca gcgcacatcc
     3721 agggcgtctc tgcgctcttt gcgcaggttg cgcttgctgc cgtcgtaatc gataaaactg
     3781 tttaggccct cttggcggct actgcttagg gcgccggtga ggctgggggt aaaggcccat
     3841 tgccagctgg cgtctaggtc tttgccgagg taattgaggt aatcaaaacg cttgtagcga
     3901 aaatccgtaa ccttggcttt gatgatgaag gtttgcaggg agtagcgttt gcggtaatcc
     3961 accgccgcga aggagccgag caccgaatcg ctgttggctt ggttggcggg cttgagaaac
     4021 acgttctctt catgctcaag ctggctaccg atctgccagc ccaaaccgta ttgttcttgg
     4081 gcctggctaa aggacgctgc taaagcgcac agggcgaaca cacagcggcg cagcgacgac
     4141 tgaaaatggg caaatccaac ggacaccaaa ggaatgttcc aaattaatag gttaaatgcc
     4201 acagactaaa gggcgggcgg gctgaggaat tcagcccagg tattcgggga gggcattcta
     4261 gccaatagtt tatagataaa gccagcaagg cccgtacttt tgtgctagag gccttgctgg
     4321 tgcggtttgc tgcaactcaa ttggctagag tttgtaaccg gcttcttcgt gcaggctgat
     4381 atccaggcct tgggattcat cttctgggct gacgcgaatg ccgttggtgc acaggcctgt
     4441 caccttcaga atgccccagg tggccgcggc ggtataaccc cccaccgcca gcataccgat
     4501 gagctgcact tttacctgcg cccagatgct ttcattgccc gcgccaaacc cgaagccgga
     4561 gaaaacacct aaatccgttg aggcaaaaat ccccaccagg aaggtgccta aaaagccccc
     4621 taccccgtgc accggaaata cgtctaagga gtcgtcgata tgtaatgtgc gcttgatcca
     4681 gtgggtggca taaaaacaaa tcaccccggc gagcaaacca atcagcaatg ccgccgccgg
     4741 gcctacgctg ccagaagccg gtgtaatggt acccaggccc gccaccatac cggtaacggc
     4801 gccgagcaca ctgggtttag aaaagcgcca ccactccata cccatccaag ctaaagcgcc
     4861 cgccgccgcg gaaaggtggg tgacgagcat agccatggac gcattgccat tggccattaa
     4921 tgcactgccg ccgttaaagc cgaaccaacc cacccagagc ataccggcgc cggtgaccgt
     4981 catggtcatg ttatggggtg gcatggcggt gttgggaaag ccgtggcggt tgcctataac
     5041 caaggctgca actaaggcag caacacctgc ggtgatatgc accaccgaac caccggcaaa
     5101 atccaacatg cccatactca tcaaccagcc gccgccccac acccaatggc ata
//
LOCUS       DAOQGS010000286         5149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8359_length_5149_cov_4.914213, whole genome shotgun sequence.
ACCESSION   DAOQGS010000286 DAOQGS010000000
VERSION     DAOQGS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_5149_cov_4.914213"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PKZ52_11175"
     CDS             complement(<1..1469)
                     /locus_tag="PKZ52_11175"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG60378.1"
                     /translation="MQAQFFTFKKTQLLAVAIASVTQAFCNSTLAAPEGAQVVAGEGA
                     IEQIEKETRVQQASERLSIDWKSFDINSDERVQFIQPTTSSIAINRILSNKGSLIQGR
                     IDANGQVVLINPNGLIFTEGASVNAGGLIASALQMSDQDYLNGRFTLNALEGSEGRVI
                     NSGLLNAATGGNISLIGQSVENKGLISANLGSVNLAAGKEAVLTFEPNGLLGVKITQA
                     SLQQDLGVDAALINSGEIKAEGGKVLLTASVSQDVFTRAVNQGELKSAKSAVVNADGS
                     FTLGGGADVINTGQVNVNGKNASAGEAVIIGGSVTNSGQITADGKTQGGHIELNSTDK
                     TEIVQQGKVTAQSSEGAGGDIKLLGNKVGVVDKADVNASGRKGGGQVLLGGDQSGKNP
                     NINNAEFLFVGEDAEVHADANELNNGGRIIAFALDTARIYGELSVKGGLAGSGGFVET
                     SGLQGLEVGLAPDITALSKNAGHWLIDPYDIVIDGNSSPS"
     gene            complement(1542..2696)
                     /locus_tag="PKZ52_11180"
     CDS             complement(1542..2696)
                     /locus_tag="PKZ52_11180"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNG60379.1"
                     /translation="MNYLAPVDTADLARAVYSVNENNPASLKIFLNNPSFSSGSAKVM
                     SAEVGFRAISATDGFGVCALGSGPYANHVIIVFRGTTEVNARGDWASNARIGVETSKT
                     CLPVHIGFNSIFKSMLPDIQAFLVQNSTTPMVHCIGHSLGGAVATLAADWVSQSFKKP
                     VRLYTFGAPRVGLEPFAKRISSRIGADNIYRVYHATDPVPMIPIYPFVHAPMPGFGHL
                     IPSSESIVSAAAHDMKKYAYSVENATWSDLNRRAPLYTVENAVEEFLKSKTQLDPANP
                     KTWAWLDASLLWILKKIGTALVATLHTTFCGADTIVDKIVWILQYGMEKIKDMKSWVL
                     RFMQKIMQALGMVVAKTAEELTKVLLTRVLHYLVVKMTSEAQKAIRNIIG"
     gene            complement(2708..3256)
                     /locus_tag="PKZ52_11185"
     CDS             complement(2708..3256)
                     /locus_tag="PKZ52_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60380.1"
                     /translation="MALVGSQYRSEEESCKKSQVAWMARGCARFAVLIIAVIFNSSCE
                     ANMITKEDVVFFSRVQGRVLFKGVPVANAKVIRRYTFDTSSPVEDSCSTDGNGVFSLP
                     MISKKDVRLTPLVQFVVHQEMYVEYDGVRHEIWLHGKMDKAENSEYGGKFRELVCELT
                     KQPSNIKLGIGEYIYTNCIWKN"
     gene            complement(3558..4100)
                     /locus_tag="PKZ52_11190"
     CDS             complement(3558..4100)
                     /locus_tag="PKZ52_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60381.1"
                     /translation="MAVADSQYRSEEENFKKSQVAWVVKNCVRCVAFIMAVIFNSSCE
                     AGMVTKEDVVLFSRVQGQVLFKGVPVSKAKVVRRYTYDTPGTVEDSCVTNDEGMFDLP
                     AIIKSNHMVVPLVQFVVHQEIYVERNGKLQPIWSHGKMKKNENSEYGGKPKKMVCELT
                     KELDNVKVGSVSSVYTSCVW"
     gene            complement(4176..>5149)
                     /locus_tag="PKZ52_11195"
     CDS             complement(4176..>5149)
                     /locus_tag="PKZ52_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60382.1"
                     /translation="GPQTVAWDNLDGRNRALVMDVDAKALVAVDLKTGLRSVLSDNST
                     QPDMPFIYDRTDYNGAPVVVDKIKKVAYVGQSSTKNTNPGVFSIALDTGVRQEALTGT
                     ARSLADLAIDYRAETTRMFDATATIGNIDFWDLTSKNAAGGYSNSQAAFPNTDFPFVN
                     SGSIALDAPRNRLLMTSLSGDQFVYSINAKAPFDSSDVNSLDRGGRSIFSNATTPNNL
                     EPFTASGTNVLTSIRIDMDRSRALMVDRVKLAVFALTISDDPTKDGARSIFSSNKESL
                     PNKLVDPYGLHIEANMDYALIVDKGQKALMALDLESGERVVVSKSKN"
BASE COUNT         1413 a         1363 c          953 g         1420 t
ORIGIN      
        1 gatgggcttg aatttccatc aataacaata tcataaggat ctatcagcca atgcccagca
       61 tttttagaga gtgctgttat atctggggct aaacccacct ccagaccctg caggccagac
      121 gtctccacaa acccgcctga accggccaaa ccacccttaa cggaaagttc accgtatatt
      181 cttgccgtat ccagtgcaaa agcaattatg cgcccgccat tatttaattc atttgcatca
      241 gcatgaacct cagcatcctc accaacaaat aaaaattctg cattattaat attgggattt
      301 ttaccggatt gatcaccacc caataaaacc tgcccaccgc ccttgcggcc tgaagcattc
      361 acgtcggctt tatctaccac ccccacctta ttacccaata atttaatatc accgcctgcg
      421 ccttcgcttg attgtgcagt tacttttccc tgttgcacga tttcagtttt atccgtgcta
      481 tttaattcaa tatgcccgcc ctgggttttg ccatccgccg ttatttggcc gctattggta
      541 acagacccgc ctataattac ggcctcaccg gcgctggcgt ttttgccatt aacatttact
      601 tgccctgtat taataacatc cgcacctccg ccaagcgtaa aactgccatc ggcatttact
      661 acagctgatt tagccgattt taattcgccc tggtttaccg cccgggtaaa aacatcctga
      721 cttacgctag ctgttaataa tactttaccg ccctcagcct taatctcgcc actgttaatt
      781 aaagccgcgt ccacgcctaa atcttgctgc aagctggctt gtgttatttt tacgccaagc
      841 aacccattgg gctcaaaggt taataccgcc tctttaccgg ccgccaaatt aacactgcct
      901 aaattagcgc taattaaacc tttgttctct acactttggc caattaaact gatattgccg
      961 ccggttgctg catttaacag cccggagtta atcacacggc cttcgcttcc ttctaacgca
     1021 tttaatgtaa acctgccatt aagataatcc tggtcactca tttgcagtgc cgaagcgatt
     1081 aatccaccgg cgtttacact tgcgccctca gtaaaaataa gcccgttggg gttaattaat
     1141 actacctgcc cattggcatc gatgcgacct tgaattagcg agcctttatt gctgagaatt
     1201 cggttaatcg cgatagacga ggtggttggc tgaataaatt gcacccgctc atcactatta
     1261 atatcgaagc ttttccaatc aatacttaag cgctcactgg cttgctggac gcgggtttct
     1321 ttttctattt gttctatcgc cccctcccca gccaccacct gcgccccttc cggcgctgcc
     1381 aaagtagaat tacaaaatgc ttgcgttacg ctagcaatcg ccacagccaa taattgagtt
     1441 tttttaaacg tgaaaaactg ggcctgcata aaacaccttg aagggggaat actttaaatt
     1501 aaaaaaggcc agcgtaagct ggcccacaaa aacagaagcc actagccaat gatatttcta
     1561 attgcctttt gtgcttcaga ggtcatttta accaccaaat aatgcaacac tcgcgtcagc
     1621 agcactttgg ttaattcctc tgcggttttg gccacaacca tacccagtgc ttgcataatt
     1681 ttttgcataa agcgcagcac ccagctcttc atatccttaa ttttttccat cccgtattgc
     1741 agaatccaca ctattttatc cacaatggta tctgcgccgc aaaaagtggt atgcaaggtt
     1801 gccaccaacg ccgtgccgat ttttttaagt atccaaagca agctagcatc caaccaggcc
     1861 caggttttag gattggctgg gtctaactgc gttttagatt ttaaaaattc ttctactgca
     1921 ttttccactg tatataacgg tgcgcggcgg tttaagtccg accaagttgc attctctaca
     1981 gagtaggcat attttttcat gtcatgcgcg gcagcactta caatgctttc gctggatggt
     2041 attaaatgcc caaaacccgg catgggtgcg tgtacaaaag gataaattgg gatcatgggc
     2101 acagggtcgg tggcgtgata tacgcggtaa atattatccg cccctattcg gctacttatg
     2161 cgctttgcaa aaggctcaag accgacgcgc ggcgcgccaa aagtatataa gcgcaccggt
     2221 tttttaaagc tttgtgaaac ccaatccgcc gcaagtgttg ccacggcgcc acctaaacta
     2281 tgtccgatac aatgcaccat aggtgtagtg gaattctgca ccaaaaaggc ttgaatatcc
     2341 ggcagcatac ttttgaaaat agagttaaaa ccaatatgca cgggcaagca agttttagaa
     2401 gtctcaacac caatacgtgc attactagcc caatctcctc tggcatttac ttcggtggtg
     2461 ccgcggaaaa ctataattac gtgattggcg taagggcctg agcctaaagc acaaacaccg
     2521 aaaccatccg tggcactaat ggcacgaaag ccgacttcag cactcattac ttttgcgctt
     2581 ccagaagaaa aagaagggtt atttaaaaat attttcagtg aggcggggtt gttctcgttg
     2641 acagaataaa ccgcacgagc caaatcagcg gtatcaacag gtgccagata attcatatca
     2701 tttcctttta atttttccaa atacagttag tgtaaatata ttcacctatg ccaagcttaa
     2761 tattgctggg ctgcttggtg agttcgcaaa ccaactccct aaattttcct ccatattctg
     2821 aattttcagc tttatccatt tttccgtgca gccaaatttc atgccgcact ccgtcatatt
     2881 cgacatacat ttcttgatgc accacaaatt gcaccaatgg cgtaaggcga acatcttttt
     2941 tggaaatcat aggcaaacta aacaccccat ttccgtcagt cgagcaagaa tcctcaactg
     3001 gactagatgt gtcgaaggtg taacggcgaa taactttggc attggctaca ggaacccctt
     3061 taaacaatac ccttccttgc acccgcgaaa aaaatactac atcttcctta gtaatcatat
     3121 ttgcctcaca cgacgaatta aaaataactg ctattatcaa tactgcgaac cgggcgcaac
     3181 ctctagccat ccaagccact tgactctttt tgcaactctc ctcctcactt cgatactgcg
     3241 aaccaactag ggccataccg ccctcctaat tattgggatg gctgactatc gccgctaggc
     3301 gataccaagc catcaaacca atggaaatcg gctaattttt ggatttggat gctactacgt
     3361 gcccgcctct ttccaaatcc atcgcggaca ttaatagtac gaaacccaac agttacactc
     3421 accacattgg tttagccagg taaaaacaaa gggtttttca aaaagtcccc gcgttaacgg
     3481 tataaactcg tgcggccaag atggctgctt cgcttggaga aatataagcc ataacctttc
     3541 cttaaaatta taattaccta ccaaacacaa ctggtataaa cggaacttac tgaaccgact
     3601 ttcacattgt ctaactcttt agttaattca caaaccattt ttttcggctt tcctccgtac
     3661 tctgaatttt catttttttt cattttccca tgcgaccata ttggttgtaa cttgccattg
     3721 cgctcaacat aaatttcttg gtgcacaaca aactgcacca acggcacaac catgtgatta
     3781 ctttttatga tagcgggcag gtcaaacata ccctcgtcat ttgtcacaca tgaatcttct
     3841 acggtgcctg gcgtgtcata ggtataacgg cgaacgactt tcgcttttga caccggaacc
     3901 cccttaaaca acacctggcc ttgcacccgc gaaaaaagca ccacatcctc tttagtaacc
     3961 atacctgcct cacatgatga attaaaaata actgccatta tgaatgctac gcaccggacg
     4021 caatttttaa ccacccaagc cacttggctc tttttgaaat tctcttcctc actacgatac
     4081 tgcgaatcag ctacagccat accgcccttc taagtattgt gatggctgac tatcaccgct
     4141 aggcgatacc aaaccatcca gccaatgaaa atcggctaat ttttggattt ggataccacc
     4201 acgcgctcgc cgctttccaa atccagcgcc attaaagctt tttgcccttt atccactatt
     4261 aaggcgtaat ccatattggc ttctatatgt agtccatacg ggtcaaccaa tttattcggt
     4321 agggattctt tattactgga gaaaatacta cgggcaccat cttttgttgg gtcatcacta
     4381 atggttagtg caaacactgc caatttcact cgatccacca ttaatgcacg actacggtcc
     4441 atatctatac gaatagacgt taatacgttg gtgccggaag ctgtaaaagg ttcaaggtta
     4501 tttggcgttg ttgcattgga aaatatcgat cggccgcctc gatccaaact attaacatca
     4561 ctagaatcaa acggcgcttt cgcatttatt gaatatacaa actgatcgcc actgaggctc
     4621 gtcataagca aacgattacg cggcgcatct aacgcaatac tgcccgagtt gacaaacggg
     4681 aaatcagtgt tggggaacgc tgcttgagaa ttgctgtatc ctccagcggc atttttagag
     4741 gtgagatccc aaaaatctat attgccgatg gtagcggtag catcaaacat acgcgtagtt
     4801 tccgctcgat aatctattgc caaatccgct aaagaacgcg cagttcccgt taatgcttcc
     4861 tgccgaacac ccgtatccaa tgcaatggaa aacactcccg gattggtatt ttttgtggat
     4921 gactgcccca cataagccac cttcttaatt ttgtccacca ccactggagc accgttataa
     4981 tccgtgcggt cataaataaa gggcatgtcc ggctgagtag agttatcact aagaacacta
     5041 cgcagaccag tttttaaatc caccgccacc aaggccttcg catccacatc catcacaagc
     5101 gcccgattac gcccatccaa attatcccaa gccaccgttt gcggcccca
//
LOCUS       DAOQGS010000287         5140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8383_length_5140_cov_4.465487, whole genome shotgun sequence.
ACCESSION   DAOQGS010000287 DAOQGS010000000
VERSION     DAOQGS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8383_length_5140_cov_4.465487"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKZ52_11200"
     CDS             complement(<1..727)
                     /locus_tag="PKZ52_11200"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNG60383.1"
                     /translation="MYDGAYRYLIDQLRQTPGKSLWFAEEGCTDYLAELAPFKDKILL
                     LSARVDIINEARQLNIPAHINDAQWPQGESPIAACFIRIAKEKPWVQHLINQARQYLP
                     AGGQLLLAGHKQEGIKTYADKAAALFLSPQKAIKNGDFYSLALTQPSVPAGSLLPCED
                     YPKFRPVFTLQEKPVYSKPGIYGWDKIDQGSQLLVDTLKAEMLPTLNNCLDLGCGYGY
                     LTLATANLDLGLRTCTDNNQAAVN"
     gene            complement(720..1484)
                     /locus_tag="PKZ52_11205"
     CDS             complement(720..1484)
                     /locus_tag="PKZ52_11205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNG60384.1"
                     /translation="MRLDKFVSHATGASRSQVHLWLRQGRISLNGTPCTQAALNIKDN
                     EQICLDEAPIQLPREGYWLLHKPAGVVCANQDGQYPTVIDLVSHALAPGNLQIAGRLD
                     VDTTGMVLITTDGAWNHQVTAPKKHCQKIYQVTLSEPLTPAAIAHLRAGIALREEPKP
                     CLPADIFALSHHEYLLAINEGKYHQVKRMMAAANSHVVKLHRLAVGGLCLNDLEPGAF
                     RPLTGDEIRQLATSPQTLNLASLAQSLFAGLEPTHV"
     gene            complement(1484..2347)
                     /gene="folD"
                     /locus_tag="PKZ52_11210"
     CDS             complement(1484..2347)
                     /gene="folD"
                     /locus_tag="PKZ52_11210"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNG60385.1"
                     /translation="MSAHNLDGNSLVLDGKSLAQKTEAELTDRVAAIKAKSGITPILA
                     TILVGDDPASATYVKMKGNACRRIGMDSLQIELPSSTTTEELLAQIHTLNMDNRVQGI
                     LLQHPVPAQIDERACFDAIALEKDVDGVTCLGFGRMAMDEEAYGSATPKGIMRLLEAY
                     DIPISGKHALVVGRSAILGKPMAMMLLAANATVTICHSRTQNLAEHIARADIVVGAVG
                     KPEFIQAAWIKDGAVVVDAGFHPGGLGDIELAPLHGRAAAITPVPGGVGPMTINTLIY
                     QTVAAAEKSLV"
     gene            3221..>5140
                     /locus_tag="PKZ52_11215"
     CDS             3221..>5140
                     /locus_tag="PKZ52_11215"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HNG60386.1"
                     /translation="MLYRCNWNGVGRVGVWGLLLIFVLVQSSRVYAQATQLTFSGAPT
                     VTAGSSGNTGSMNTKARWVNVATVNGVAVDMEAVILANSVATNNSVSWSTVSGQAKVA
                     LVGEGDQSVDLAYHFYQTGTNTPVVVAPEVIFRGLDGGLRQEVIQTLKTQIAKYTLDS
                     TSSISVSSVLNGAGASDDEYKFTSTSGTNLVTNSGFESGNTGFTSSYTYLGEHGNNNS
                     IAQGSWGEGKYAWFNDNRTPNSSNSYFNLTTANNGDAFLVVDIGNDTTHPFWQTSLSL
                     TPGKNYVFSAFLANINDSNSNIKPNVNFVLTNSGGTSVLAPSGNLNTGGASLTPWQEV
                     TSSFTASVASNTLALISNTAGLFGNDLAMDDVEVREVLPENQTGLSLTLQPASVFYFT
                     FKKSNGLGEFALDGSLSAFFSSPQATLVDTTAPATPSVTSLTTTDTTPVLSGSAEAYS
                     SVTLSVGGATYATSANGSGAWSVDTGTATPASGAFAPLTNGAANSVQVVSTDAAGNAS
                     SPGTGTLIIQVPAASNSSITPSPSTIAATGNATTTITVQAKNASNSNLTVGGSSVVLS
                     TNLGSLGSVIDNGDGTYTASLTSATAPGTATIDQLGEGIHRGIARGGRELGGDPANLP
                     RILLEPLGDRVDERLI"
BASE COUNT         1083 a         1280 c         1494 g         1283 t
ORIGIN      
        1 catttaccgc cgcctggtta ttgtcggtgc aggtgcgcag gcctaagtct aaattagccg
       61 tggcaagggt taaatagccg tagccgcagc ctaaatccag gcagttattt agggtgggaa
      121 gcatctcggc cttgagtgta tcgaccaata attggctgcc ctgatcgatt ttatcccagc
      181 cgtaaatacc gggttttgag tacacgggct tttcctgcag ggtaaaaact ggcctgaatt
      241 ttgggtagtc ctcgcagggc agaaggctgc ctgcgggcac gcttggttgt gtgagcgcta
      301 ggctgtaaaa gtcgccattt ttaatggcct tttgtgggct taaaaataga gcggcggctt
      361 tatccgcgta ggtttttatg ccttcttgtt tgtggccggc caacaataat tggccgcccg
      421 ctggtaaata ctggcgggct tgattaatta aatgctgcac ccagggtttt tctttggcga
      481 tgcgaataaa acaggcggca atgggtgact cgccttgggg ccattgggcg tcgttaatgt
      541 gcgcgggaat attaagttgc cgggcctcat taataatatc cacccgggcg ctgagcaaga
      601 gtattttgtc tttaaagggg gcaagttcgg ctaaataatc cgtgcagccc tcttcggcaa
      661 accaaaggct tttgcccggt gtttggcgca attggtcgat taaatagcgg taggcgccgt
      721 catacatggg tgggttctag gcctgcaaaa agggattggg caaggcttgc caggttaagg
      781 gtttgggggc tggtggccaa ttggcgtatt tcatccccag tgagggggcg aaaggcgcca
      841 ggttctaaat cgtttaagca taggccgcca acggctaagc ggtgcagttt aaccacatgg
      901 gagttggccg ccgccatcat gcgtttaacc tgatggtatt tgccttcatt tattgccagt
      961 aaatattcat ggtggctgag cgcaaaaata tccgcaggca ggcagggttt tggctcctca
     1021 cgcagtgcaa ttcctgcgcg caagtgcgct atggccgctg gggtaagggg ctcgcttaag
     1081 gttacttggt aaattttttg gcagtgtttt ttgggggcag taacctggtg attccaagcg
     1141 ccatcggtgg tgatcagcac catgccggtg gtgtccacat ctaggcggcc agcaatttgt
     1201 agattgcctg gcgctagggc gtggctcacc aaatcgatca ctgtggggta ttggccgtct
     1261 tggttggcac agaccacacc ggcgggtttg tgcagcagcc aataaccttc cctggggagt
     1321 tggatcgggg cctcatccag gcaaatctgc tcgttatctt tgatattcag cgccgcttgt
     1381 gtgcaggggg tgccgttcag gctgatgcgc ccttgccgca gccaaaggtg cacttggctg
     1441 cggctggcgc cggtggcgtg gctgacgaac ttgtccagcc gcattaaacg aggctttttt
     1501 ctgcagcggc cacggtttgg taaatcaggg tgttgatggt catggggcct acgccgccgg
     1561 gtacaggcgt gatagctgcg gcgcgcccgt gtagcggtgc tagttcaata tcgcccaggc
     1621 cgccggggtg gaagcctgcg tctaccacta cagcgccatc tttaatccag gcggcttgga
     1681 taaattcggg cttgcccacg gcgcccacga ctatgtcggc acgggcgata tgctcggcta
     1741 aattctgggt gcgggagtgg cagatggtga cggtggcgtt agcggccagc agcatcatgg
     1801 ccatgggttt gccgaggatg gcgctgcggc ccactaccag ggcgtgtttg ccgctgatgg
     1861 gtatatcgta ggcctcaagc aaacgcatga tgcccttggg ggttgcgctg ccgtaggctt
     1921 cttcgtccat ggccatgcgg ccaaatccca gacaggttac gccgtctacg tctttttcta
     1981 gggcgatggc gtcgaaacag gcgcgctcgt cgatctgtgc aggcaccggg tgttgcagga
     2041 gtatgccctg cactcggttg tccatattga gggtgtgaat ttgcgcgagt aattcttcgg
     2101 ttgtggtgct ggagggcagt tcaatttgta gggaatccat gccgatgcgg cggcaggcgt
     2161 tgcccttcat tttgacgtag gtggctgagg ctgggtcgtc tcccaccaag atggtggcga
     2221 gtatgggggt tatgccggat ttggctttga tcgccgctac gcggtcagtg agctcggctt
     2281 cagttttttg cgccaggctt ttgccgtcga gaaccaggct attgccatcg agattgtgtg
     2341 cgctcataca aggctccaag gaaaatggcg ggcattttca cacaggcgcc tggcttcctc
     2401 aatcgcgctg cggttggcct ggcgcgtgtg ggcgcctaat ccggtgtaaa agctcaaaga
     2461 aaactgggca acatcaagtg ttgctcctct gttttggggg ttgcggcctg ttgttagata
     2521 ggctgggtaa attactcctc actctatgga aatagaccag cccatcaccc cctgaccggc
     2581 gcgcctttag gtatcccctg tcagaataaa cgctacgcac aacgatggcg ccgcggggtg
     2641 tcggaagtcg ctacgctctt ccaacttacg ccagagtggg ctttttcctt aacttaatga
     2701 catgcctgtg caggaggaga atttagccaa acggcacaat ctaaaaaatt taagacaacg
     2761 gattcgcggt gcagcagtgg tgaatacagg ttattgcaga ccgtcaaaaa gagggacctt
     2821 tttgacgagc ctacacggat gtatccgcgg cgtgtctgca ataacctgta ttcaccactg
     2881 cgggtggact attgatggtt cgcagcacta aagcactcgc aataaaaaag cactgaataa
     2941 ttgcgttttt ttgtatgccg ccgggttaat tcgtctcgcc ctgtaagcca tatttttggc
     3001 gaattatttt tttattcgca gcacctatta aaaatgccat ttttttgtat tcataaaaag
     3061 tggattattt tttgccgaaa aagaggattt gtgtgacggt attcaaagtg cttgctatat
     3121 tcaaagtgct tgctatattc aaatgaagca agtattgcgg tggttttaat tctgtaagtg
     3181 aaactgccac catcatttaa ctttcaccgt tgggggccac atgttgtatc gatgtaactg
     3241 gaatggagtg ggcagggtag gcgtttgggg gctgttgctg atttttgtgc tggtgcaatc
     3301 aagccgggtg tatgcccagg ccacccagct aacttttagc ggtgcgccaa cggtgacggc
     3361 gggcagcagt ggcaataccg gctcgatgaa caccaaagcc cgctgggtga atgtggccac
     3421 ggtgaatggt gtggcggtgg atatggaggc ggtgatactg gccaattctg tagccacgaa
     3481 taactccgtc agttggtcta ctgtgtccgg ccaggccaag gtggcgctgg tgggggaggg
     3541 ggatcagagt gttgacctgg cctatcactt ttatcaaacc ggcaccaaca caccggtggt
     3601 ggtggcgccg gaggttattt ttcgcggttt ggatggcggt ttgcgccagg aagtaattca
     3661 aaccttaaaa acacaaattg ctaaatacac gcttgatagc acttcttcta tttcggttag
     3721 ctccgtgctc aacggtgcgg gtgccagtga tgatgaatat aaatttacct ccacctccgg
     3781 cactaattta gttaccaatt ctggctttga aagtggcaat acgggtttta catccagtta
     3841 cacctattta ggtgaacacg gcaataataa ttccatcgct cagggcagtt ggggggaggg
     3901 caaatacgct tggtttaatg acaaccgcac gcctaattcc agcaattcat attttaattt
     3961 aaccaccgcc aataacggcg atgccttttt ggtggtggat attggcaacg atacaaccca
     4021 cccgttttgg cagacgtcgt taagccttac gccgggtaaa aattatgttt tctcggcgtt
     4081 tctcgccaac attaacgata gtaattccaa tatcaaaccc aacgttaatt ttgtgctgac
     4141 taattccggc ggcaccagtg tgttggcgcc ttcgggtaat ttaaataccg gcggtgcaag
     4201 ccttacaccc tggcaggaag ttaccagttc atttacggcc agtgtggcca gcaatacatt
     4261 ggcgttaatt agtaatacgg cgggtttatt cggcaatgat ttggccatgg atgatgtgga
     4321 agtgcgggag gtgttgccgg agaatcaaac cggtttgagc cttacgctgc agcctgcctc
     4381 ggtgttttat ttcaccttta aaaaatccaa tggcttaggt gagtttgcgt tggatggctc
     4441 gttaagcgcg tttttttcaa gcccgcaggc caccctggtg gataccactg cgcctgccac
     4501 ccccagcgtg accagcctca ccaccaccga tactaccccg gtgctgagtg gcagtgcgga
     4561 ggcctattcc agtgtgaccc ttagcgtggg cggcgcaacc tacgccacca gtgccaatgg
     4621 cagcggcgcc tggagtgtgg ataccggcac ggctacaccg gccagtgggg cctttgcgcc
     4681 cctcaccaat ggtgcggcca atagtgtgca ggtggtgagc accgatgcgg cgggcaatgc
     4741 ctccagccca ggtaccggca cgttgattat tcaggtgccc gccgccagca actccagcat
     4801 taccccaagc cctagcacca tcgctgccac cggcaatgcc accacaacaa ttaccgtaca
     4861 ggcgaaaaat gccagcaata gcaacctaac ggtggggggc agttcggtgg tgctcagcac
     4921 caacctcggc agcctcggca gtgttattga taacggcgac ggcacctata ccgccagcct
     4981 tacctccgcc acagcgcccg gcaccgccac catcgaccaa cttggcgagg gcatccaccg
     5041 cggcatcgcc cgcggcggcc gcgagctcgg cggcgatccc gcgaacctcc cacggatcct
     5101 tctggagccg cttggcgatc gggtcgatga gcgcttgatc
//
LOCUS       DAOQGS010000288         5125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8423_length_5125_cov_4.036095, whole genome shotgun sequence.
ACCESSION   DAOQGS010000288 DAOQGS010000000
VERSION     DAOQGS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8423_length_5125_cov_4.036095"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKZ52_11220"
     CDS             <1..288
                     /locus_tag="PKZ52_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNG60387.1"
                     /translation="HFFDALVSSHDYQAPKESALFWQAITEQFNLNPARCLFVDDTPR
                     ILHAAKNFGIGYLVHINQPDSKQSANPCSDFLSLPDFASLIPSLHAGGHNG"
     gene            281..685
                     /locus_tag="PKZ52_11225"
     CDS             281..685
                     /locus_tag="PKZ52_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNG60388.1"
                     /translation="MAERAPLTAVRIDKWLWAARFFKTRALAKDAIEGGKVQVNGQRV
                     KPSKDVGLQMTLSIRQGFEEKTVIITGLSEQRGNATLAATLYSETPESLEKRLLMQEQ
                     RKLQQAAQPLTEGRPTKKQRRQIHQFILHHSD"
     gene            730..1617
                     /gene="hslO"
                     /locus_tag="PKZ52_11230"
     CDS             730..1617
                     /gene="hslO"
                     /locus_tag="PKZ52_11230"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNG60389.1"
                     /translation="MNSLDTVQRFYFDNTDLRGEIITLHKTLLDAMAHHHPSPTCRTL
                     LGEFFAAAGLIAGILKFEGILTLQVRGDGPISLIMAEATQERCLRGIVHTQDEIVEAE
                     LTGKSLQELLGSGVLTITLDPTEGQRYQGIIALEGATLAEALSDYFAQSEQLPTRLWL
                     EADSTKAAGLLLQALPPTGERKHNPELREEEFNGALQLANTISRDELLNLSHEEILYR
                     LFNSYSLRLMPEITTQFRCSCSKARSGNALISLGMQDAYDLLAEQKVIGIDCQFCGQH
                     YSFEQKDLDAIFHVEGGAH"
     gene            1649..2311
                     /locus_tag="PKZ52_11235"
     CDS             1649..2311
                     /locus_tag="PKZ52_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG60390.1"
                     /translation="MPTVVITGANRGIGLALAQEYSKQHWQVIALCRATSAELAATGA
                     QVITGLDLKNLQGLAQIVQELKHQNVDVLINNAGILGREHLGDLDTASITEQFIVNAL
                     APIKVAEAFLPLLNPGAKVAFITSRMGSIADNTSGGFYGYRMSKAALNAAAKSFSEDV
                     KSKQIAVGILHPGYVQTAMVNFGGDISAEVCAGRLVQRIADLNIGNTGSFWHSNGDLL
                     PW"
     gene            complement(2865..3665)
                     /locus_tag="PKZ52_11240"
     CDS             complement(2865..3665)
                     /locus_tag="PKZ52_11240"
                     /inference="COORDINATES: protein motif:HMM:NF028266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HNG60391.1"
                     /translation="MEKIHRLSVSLLVSGLILSAPVIASEYRSEISLSYAQNKANSNL
                     YSAKDRVYFSGANIYFTPVETQSHPLAEAAFLGEHSFVSLDYYKYNSKYRLGFENPVE
                     VESKDSYSSIYTRIYVPNTPLVLGYGDSLSEDDVTGTYTIGLAPAEGLLLYTLYWRDD
                     ETNSRPNLYAEYVQPLANTQALRINAGFTNAPDDQSNVYELSGDYYFNSRWSVGLAWS
                     GQGDYSSYGLSSRYFFAEQFSVSADYNYSKSSDDLSGRTLSAGVEVRF"
     gene            complement(3934..4572)
                     /gene="tmpT"
                     /locus_tag="PKZ52_11245"
     CDS             complement(3934..4572)
                     /gene="tmpT"
                     /locus_tag="PKZ52_11245"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNG60392.1"
                     /translation="MDADFWQQKWHNNDIGFHQSEGNPLFIKYFNALGVPPGGRIFLP
                     LCGKTRDIAWLLSQGYKVVGAELSALAIEQLFAELGVQPTRAYFGNVVLYRAPNIDIF
                     VGDIFALTHLMLGKVDAIYDRAALVALPENKRKFYSSHLIDIAARVPQLLICFEYDQS
                     QMPGPPFAILEAEVKSHYQQYYGLTLLEKVPVEGGLKGSCEALEVVWLLKTK"
     gene            complement(4628..5080)
                     /gene="coaD"
                     /locus_tag="PKZ52_11250"
     CDS             complement(4628..5080)
                     /gene="coaD"
                     /locus_tag="PKZ52_11250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009021231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNG60393.1"
                     /translation="MTITNGHVDIVERACRLFDEVVVAVAASPRKKPLFSLEQRVDLC
                     REVLKDFPQVQVCGFDGLLATLVAEKHACGVVRGIRAVSDFEYEFQLANMNRALDENL
                     ESLFLTPSEHLSFISSSLVKEIASLNGAVEKFVPPSVAVALLDIFKNS"
BASE COUNT         1445 a         1314 c         1145 g         1221 t
ORIGIN      
        1 catttttttg acgccctggt gtcctcccac gattatcaag caccgaaaga atccgccctg
       61 ttttggcagg cgattaccga gcaatttaat ttaaacccag cgcgctgttt atttgtggat
      121 gacacgccgc gcattttgca cgccgccaaa aactttggca taggttattt agtgcatatt
      181 aaccagccgg attccaagca gagcgccaat ccctgtagcg attttttaag cctgcctgat
      241 tttgcaagcc tcatacccag cttacatgca ggaggccaca atggctgagc gcgcaccgct
      301 taccgctgta aggattgata aatggctgtg ggctgcgcgt ttttttaaaa cccgcgcgct
      361 ggccaaagac gccattgaag gcggcaaagt gcaggtaaat ggccagcggg taaaacccag
      421 caaggacgta ggcctgcaga tgaccctcag cattcgccaa ggatttgagg aaaaaaccgt
      481 tattattacc ggcttaagcg agcagcgcgg caacgccact ctcgctgcca ccctctacag
      541 cgaaacaccg gaaagcctgg agaaacgcct gctaatgcaa gagcagcgca aactgcaaca
      601 ggccgcacaa cccctaaccg aagggcgccc caccaaaaaa cagcgccggc aaattcacca
      661 atttattttg caccacagcg attaaaccaa cctgcgcgtt tgcgcacgaa tagcacaacc
      721 gagataccta tgaattccct ggatactgta cagcgttttt attttgataa taccgatttg
      781 cgcggtgaaa tcattacgct gcataaaacc ctgctcgacg ccatggccca ccatcacccc
      841 agccccacct gccgcaccct attgggggaa ttttttgccg ccgccggttt aattgccggt
      901 attttaaaat tcgaaggcat tttaactcta caagtgcgcg gcgacggccc cattagctta
      961 attatggccg aagccaccca agagcgctgc ctgcgcggca tagtgcacac tcaagacgaa
     1021 attgtagaag cggaattaac cggtaaaagc ctgcaggaat tattgggcag cggcgtatta
     1081 accattacgc tggaccccac cgagggccaa cgctaccagg gcattatcgc ccttgaaggc
     1141 gccaccctgg ccgaggccct gagtgattat tttgcccaat ccgaacaatt gcccacgcgc
     1201 ttatggcttg aagccgacag cactaaagcc gccggtttat tactgcaggc cctgccccct
     1261 accggtgagc gcaaacacaa cccggaatta cgcgaagaag aatttaacgg cgccctgcaa
     1321 ttagctaata ccattagccg cgatgagtta ttaaacctaa gccacgaaga gattttatac
     1381 cgcttattta atagctatag cctgcgttta atgccggaga ttaccactca atttcgctgc
     1441 agctgctcta aagcgcgtag cggcaatgcg ctgatttccc tcggcatgca agatgcctat
     1501 gatttattgg cggagcaaaa agtcattggc atcgactgcc aattttgcgg ccagcattat
     1561 agttttgagc aaaaagactt ggatgccatc ttccatgtgg aaggcggcgc ccattaatca
     1621 gtaacgtgtt ttagctgagg agtttcctat gcctactgtc gtaattaccg gcgctaaccg
     1681 aggcattggc cttgcactgg cacaagaata cagcaagcag cactggcaag taattgccct
     1741 gtgccgcgcc acaagtgcag aacttgccgc caccggcgcc caggtgatca cggggctgga
     1801 tttaaaaaac ctgcagggcc ttgcgcaaat tgtgcaggaa cttaagcacc aaaatgtaga
     1861 tgttttaatt aataacgccg ggattttggg ccgtgagcat ttaggtgatt tagataccgc
     1921 cagcataacg gagcagttta tcgtcaacgc attagcgccc ataaaagtgg ccgaagcctt
     1981 tttaccccta ttaaatccag gcgctaaagt agcctttatt accagccgca tgggctccat
     2041 cgccgataat acttccggtg gtttttacgg ctaccgcatg agtaaagcgg cccttaatgc
     2101 ggcggcgaaa tccttcagcg aagacgttaa aagtaagcaa atagcggtgg gcatattgca
     2161 cccgggttat gtgcaaacgg ctatggtgaa ttttggtggc gatatttccg ctgaggtgtg
     2221 tgcaggaagg ttagtgcagc ggattgcgga tttaaatata ggaaataccg gcagcttttg
     2281 gcattccaat ggggatttgt tgccttggta attaaagcac tttgccgagc aacaaaaacg
     2341 acttgtgatt ggatttattg caagaagctt ttttcagagt aaatttgtgg caagccatcc
     2401 tcagggctac accccaaaaa aacctttgcc caactgggca ccctgaccag ttttattaaa
     2461 aaggcaagag ctgaatcaaa cccaacccgc agaaaaatta acgctacacc ctctagcctt
     2521 tattttatgc agggtaattt tttatgcagt actctttttt tgccttggca acttccatgc
     2581 taaccgagat cactcctgcg agcggctgag caggggcgaa tgattgccac ccatccccca
     2641 gcaataaata cagcttaatt tagacatgcc atgaatacat cttaagtagg ctcgtcaaaa
     2701 agatccctcc tcttggcgag ccgaaataag ccatattcat tgctgccgca tcgcgaccta
     2761 gctaagcaga ttggattaat tttacttaat ttgccacctg tgcgaagagg gtaaaaaagg
     2821 ctgattcgtt ctgataaatt tgcgtcaccc gctgaaaccg agaactaaaa ccgcacctca
     2881 acacctgcgg agagagtacg cccacttaaa tcatcgcttg acttgctgta attgtaatca
     2941 gcggatacgg agaattgttc cgcaaagaaa taacgactgc tcaatccgta acttgagtag
     3001 tcaccttgcc cagaccaggc aagacctaca gaccatcgag aattaaagta gtaatcacct
     3061 gaaagctcat acacattgct ttgatcatct ggcgcattgg taaagcctgc atttattctc
     3121 aatgcctgcg tattcgccaa gggctgcaca tactcggcat aaagattagg ccggctattt
     3181 gtctcatcgt cgcgccaata gagggtatat agcaaaagcc cctcagcggg ggccagccct
     3241 attgtatagg ttccggtgac atcatcctct gacaatgaat caccataacc taaaaccaat
     3301 ggagtattgg gcacgtaaat tcgcgtgtag atagaagagt aagaatcttt gctctccacc
     3361 tctactggat tttcaaagcc cagccgatat ttagagttat atttgtaata atcaaggctt
     3421 acgaagctat gttcgcccag gaaagccgcc tctgccagtg ggtggctttg ggtttcaaca
     3481 ggtgtgaaat aaatatttgc acctgagaag tagacgcgat ctttagccga atataaattg
     3541 ctattggctt tattctgtgc atagctaagt gatatctcac tgcgatactc actagcaatc
     3601 acaggcgcag acaaaattaa accagatact aacaatgaaa cagataaacg atggatcttc
     3661 tccatacttt ctccttaaat ataaaacaag aaaaataata aaagcctcaa caattcagca
     3721 caccaaacca aacaattggc aagccaaact gattaatact tttaaaacca agctcaagca
     3781 acacctctat aaacaagggc acttcaaagc aatacgattt acatcaaagc aggcaatacg
     3841 gcaaagatag atcggcgccc aataacaata ctccataacg tacaaagcat acgtatgttg
     3901 tgcagtcttt cacgtactgt ctgcaaatca tggctatttg gtttttaaca accaaaccac
     3961 ttccaaagcc tcacaactgc cctttaaccc accctccacc ggcaccttct ccaacagagt
     4021 taacccataa tactgctgat aatggctttt tacttccgct tccaaaatag caaacggcgg
     4081 gccgggcatt tgggattggt cgtattcaaa acaaattaat agctggggta cacgggcggc
     4141 tatatcgatt aaatggctac tgtaaaattt gcgtttattt tcgggtaggg cgaccagtgc
     4201 tgcgcggtcg taaatagcgt ctactttgcc cagcattaaa tgggtgagtg caaaaatatc
     4261 gccgacaaaa atatcgatat tcggcgcccg gtataacacc acattgccaa aatatgcccg
     4321 agtaggttgc acgcctaatt cggcaaataa ctgctctatg gcaagcgcac tcagctccgc
     4381 gcccaccacc ttgtagccct gggacaagag ccaggcgata tcgcgggttt tgccgcacag
     4441 aggcagaaaa atccgcccgc caggtggcac accaagagca ttaaaatatt taataaatag
     4501 aggattaccc tcgctctggt gaaaccctat gtcgttgtta tgccacttct gctgccaaaa
     4561 atctgcgtcc ataattcctc gcaaatatta atcaatgcgc aaacgctccc ggcgccggga
     4621 ccattaatta tgaattttta aaaatatcca gcagggctac agccacactg ggcgggacaa
     4681 acttttccac cgcgccattt aaagaagcaa tttcttttac taaagacgac gaaataaagg
     4741 ataaatgctc ggagggcgtt aaaaacaagc tctccaaatt ttcatccagg gcgcggttca
     4801 tattggccaa ttggaattcg tattcaaaat ccgacacggc gcgaatgccg cgcaccacgc
     4861 cgcaggcgtg tttttcagcc accagagttg ccagcaggcc gtcaaagccg cacacctgca
     4921 cttgcgggaa atcttttaac acctcgcggc ataaatctac tcgctgctcc aggctaaaca
     4981 agggtttttt gcgcgggctg gcggctaccg ccaccactac ttcatcaaat aatcggcaag
     5041 cgcgctcgac tatgtcgaca tggccgttgg tgatggtcat gggaaaggcg ttaggcgtta
     5101 cgggaaggat cgatctggtt gtagc
//
LOCUS       DAOQGS010000289         5113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8446_length_5113_cov_4.996046, whole genome shotgun sequence.
ACCESSION   DAOQGS010000289 DAOQGS010000000
VERSION     DAOQGS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8446_length_5113_cov_4.996046"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /gene="ccoS"
                     /locus_tag="PKZ52_11255"
     CDS             complement(<1..149)
                     /gene="ccoS"
                     /locus_tag="PKZ52_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNG60394.1"
                     /translation="MYFLIPISFLFVALAIGLYIWAVHSGQYDDLDVEGQRILFDQDD
                     PPQQNP"
     gene            complement(185..1327)
                     /gene="proB"
                     /locus_tag="PKZ52_11260"
     CDS             complement(185..1327)
                     /gene="proB"
                     /locus_tag="PKZ52_11260"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNG60395.1"
                     /translation="MTAATNAQNRRTLIGQSPRLVVKIGSALLTNEGRGLDKTAIAGW
                     VAQMAAIVNAGHELVLVSSGAVAAGMTRLGWKERPSSIHELQAAAAVGQMGLIQTYEE
                     AFGAFGIATAQVLLDHDDLSSRERYLNARSTLRTLTGLKVVPVVNENDTVVTDEIRFG
                     DNDTLGALVANLIEADTLILLTDQLGMYNADPRKHPDAVILSERAASDAALDAMAGGG
                     GVLGRGGMATKLRAARLAARSGANTLIVGGRIPDVLTRVMQGEDLGTLLYAQEAPMAA
                     RKQWLAGQLQVRGTLELDEGAVQVLTTSGKSLLPVGVKQVQGQFTRGDLVSCRGPDGR
                     EIARGLVNYHADEARKIAGKPSSHIADLLGYKDYDELIHRDNLILV"
     gene            complement(1367..2593)
                     /gene="cgtA"
                     /locus_tag="PKZ52_11265"
     CDS             complement(1367..2593)
                     /gene="cgtA"
                     /locus_tag="PKZ52_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNG60396.1"
                     /translation="MKFVDEAPIHVKAGRGGKGCLSFLREKFITKGGPNGGDGGDGGS
                     VFLEADEGLNTLIDYRFSRTFQAEDGEAGRGTNCTGKKGDDLILKVPVGTSVIDEDTG
                     EVIGDLTEPGERLLVAQGGWHGLGNTRFRSSVNRTPRQTTPGSPGEERNLRLELKVLA
                     DVGMLGLPNAGKSTFIRAVSSAQPKVADYPFTTLVPSLGVVKVNRHRSFVIADIPGLI
                     EGASEGAGLGIRFLKHLTRCRILLHLVDMYPIDGTDPLENARAIINELEKFSPTLAER
                     ERWLVLNKADLIDAEEAEAIKDTVVAGLNWQGPVYVVSAANRLGTEVLCEHLLTHLEE
                     QWEAERESAELAEAEKERQLIMQKEAREHIRELRERHFEKRRALKGKGGDDLSDDLDD
                     DDWNEDDYDVEFEYAP"
     gene            complement(2730..2993)
                     /gene="rpmA"
                     /locus_tag="PKZ52_11270"
     CDS             complement(2730..2993)
                     /gene="rpmA"
                     /locus_tag="PKZ52_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNG60397.1"
                     /translation="MAHKKAGGSTRNGRDSESKRLGVKAFGSQLVNAGSIIIRQRGTQ
                     YHPGENVGIGKDHTIFATKTGYVSFAVKGANNRRVVSIIEATA"
     gene            complement(3009..3320)
                     /gene="rplU"
                     /locus_tag="PKZ52_11275"
     CDS             complement(3009..3320)
                     /gene="rplU"
                     /locus_tag="PKZ52_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNG60398.1"
                     /translation="MYAVIVSGGKQHRVVEGEVLRLEKLEAETGASINFDQVLMVVNG
                     SDVKIGAPVVAGATVKAEVISHGRADKVTIIKFRRRKHSMKRQGHRQWYTEVKITGIN
                     G"
     gene            complement(3539..>5113)
                     /locus_tag="PKZ52_11280"
     CDS             complement(3539..>5113)
                     /locus_tag="PKZ52_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG60399.1"
                     /translation="AGCLVLALVGIWGGVEQEGVIKRMEYVLHHKSEVNESVDHLNVV
                     FKAQVQEWKNVLLRGSNPSDGQKYWQAFTEQHKKFEAKVEEAVKLLDDDPLAKDLRNL
                     STLHSALLPKYEEGRRAFEQAGFDAKVGDSAVKGIDRELSQKIDALADMLNASMEKDT
                     QALSAAAGAIYGRTLVVELVLTALVVFAVVVSVKSQVLNPLAQVIDHIRRLAEGDYTR
                     SLAAVREDELGALFNNVEKLRGALCSMIGGVRSTAMVLTQVNEQLNQSSIGLDRDTAK
                     TEGYAGQIATAINEMVSTVSEVAKNASQAADATVLAETRLHEGNQIMSGAISAITRVA
                     DEVAQTSEQMNRLKEESTSVGAVLDVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVV
                     ADEVRALAKRTQESTEEIQQIIKALQDGASSAAQAMLQSTGKTRDSVAQAENAGRTMS
                     DISGAIGLIRDMTHQIAAAAQEQSHAADEINRNVVSMASLAENAHAHARKTGAVTQGL
                     DQTAKDLQSMVAGFRL"
BASE COUNT          969 a         1521 c         1455 g         1168 t
ORIGIN      
        1 gggttctgct gcggcgggtc gtcttggtcg aagagaatgc gctggccctc aacgtcgagg
       61 tcatcgtact ggccgctgtg cactgcccag atatacagac caatggccag cgccacaaac
      121 aggaaggaga tagggattaa aaaatacatg ctttccacgg actactcctc gggaagagcg
      181 gggtttacac caggattaaa ttgtcccggt gaatcagttc gtcgtagtcc ttatagccca
      241 gcaggtcggc gatgtggctg ctgggtttac ccgcgatttt gcgggcttca tcggcgtggt
      301 aattcaccag gccacgggcg atctcgcggc catccggccc ccggcagctg accaaatcac
      361 cgcgggtaaa ctggccttgc acctgtttca cccccaccgg cagcaggctt ttgccgctgg
      421 tggtgagcac ctgtacggcg ccctcgtcca attccaatgt gccgcgcacc tgcagctgcc
      481 cggccagcca ttgtttgcgg gctgccatgg gagcctcctg ggcgtagagc aatgtgccta
      541 agtcttctcc ctgcattaca cgggtcagca catccgggat acggccgccg acgattaggg
      601 tattggcgcc tgagcgggca gccaagcggg cggcgcgcag tttggtggcc atgccaccgc
      661 gccctaacac accgccgcca ccggccatgg catcaagcgc cgcatcggag gcggcgcgct
      721 cgcttaagat caccgcatcc gggtgtttgc gcggatcggc gttgtacata ccgagctggt
      781 cggtaagcaa aataagcgta tccgcttcaa ttaaattcgc caccagggcg ccgagggtgt
      841 cgttatcgcc aaagcggatt tcgtcggtaa ccaccgtgtc attttcattc accaccggca
      901 ccactttaag gccggtaagg gtgcgcaggg tggagcgggc gtttaggtag cgctcgcggc
      961 tggagaggtc gtcgtggtcc agcaacacct gggcggtggc tatgccaaag gcgccaaagg
     1021 cctcttcata ggtttgaatc aaacccattt ggcccacggc ggcagcggct tggagttcgt
     1081 ggatgctgct ggggcgctcc ttccagccta agcgggtcat gcctgcggcc acggcgccgg
     1141 aggacaccag caccaactcg tggcccgcgt tcactatggc cgccatctgc gccacccaac
     1201 cggcgatggc tgttttatcc aggccccggc cctcattggt gagcagggcg ctgccaattt
     1261 tcaccaccaa acgcgggctt tggccgatta aagtgcggcg gttttgggcg ttggtggctg
     1321 cagtcatagg gggcctcagg ttagcttttc gtattaggtt tagctgttaa ggggcgtatt
     1381 caaactcaac gtcgtagtcg tcttcgttcc aatcgtcgtc atccaggtca tcacttaaat
     1441 catcgccgcc cttgccttta agggcgcggc gtttttcaaa gtggcgctca cgcaattcgc
     1501 gaatgtgctc ccgcgcttct ttttgcatga tgagctggcg ctctttctcg gcttcggcca
     1561 gctccgcact ctctcgctcc gcttcccatt gttcttccaa atgggtgagc aggtgttcgc
     1621 aaagcacttc ggtgcccagg cgattggcgg cggagaccac ataaacgggc ccctgccagt
     1681 taaggcctgc taccaccgtg tctttaatgg cttcggcttc ttcagcatcg atcaaatccg
     1741 ctttgtttaa cactagccag cgctcccgct cggcaagggt ggggctgaat ttttctaact
     1801 cattgatgat ggcgcgggcg ttttccaatg ggtcggtgcc gtcgatgggg tacatgtcca
     1861 ccaggtgcag cagaatgcgg cagcgggtta agtgctttaa aaagcgaata ccgaggcccg
     1921 cgccttcaga ggcgccttcg atcaaaccgg ggatatcggc aattacaaag ctgcggtggc
     1981 ggtttacttt taccacgccg aggcttggca ccagggtggt aaaggggtaa tcggccactt
     2041 tgggctgggc ggaggacacg gcgcgaataa aggtggattt accggcattg ggcaaaccca
     2101 acatgcccac atccgccagc acttttaact ccaggcgcag gttgcgctct tcgccggggc
     2161 tgccgggggt ggtttggcgg ggggtgcggt ttacgctgga gcgaaagcgc gtgttgccga
     2221 ggccatgcca accgccctgg gctaccaaca ggcgctcgcc gggctcggtt aaatcgccaa
     2281 tcacctcgcc ggtgtcttcg tcgatcaccg aggtgcccac gggtactttt aaaatcaggt
     2341 catcgccttt tttgccggtg cagttggtgc cccggcccgc ctcgccgtct tcggcttgaa
     2401 aggtgcggga aaagcgatag tcgatgaggg tattgaggcc ctcatcggct tcgagaaaca
     2461 ccgagccacc atcgccgccg tcgccgccat tggggccgcc tttggtaatg aatttttccc
     2521 gcaggaacga cagacagcct ttgccgccac gcccggcttt cacgtgtatc ggtgcttcat
     2581 cgacgaattt catactaact accttgccgc cacagcgtgc ggcattcaaa ttgactcacc
     2641 ttggcggcgc cgcaaaagcc tgcgccagaa acaaaaaagc cccgtcgcca aggagacagg
     2701 gcttcgcacc aattacttgg tgaattgaat taggcagtcg cctcaatgat gctgactaca
     2761 cggcgattgt tagcgccttt caccgcaaaa ctgacgtagc cagttttggt ggcgaagata
     2821 gtgtggtctt tgccgatgcc tacgttctca cctgggtgat actgagtgcc gcgctgacgg
     2881 atgataatgc tgcctgcgtt caccaattgg ctgccgaagg ctttgacgcc taagcgttta
     2941 ctttccgagt cgcgaccgtt gcgggtacta ccgcctgctt tcttgtgggc catgattcaa
     3001 atcctcaatt aaccgttaat gccagtaatt ttaacttcgg tgtaccactg acggtggccc
     3061 tgacgcttca tcgagtgctt acgacggcgg aacttgatga tggtcacttt atcggctcgg
     3121 ccgtggctga tgacttcagc tttaacggtt gcgccagcta ctacaggggc gccgattttt
     3181 acgtcgctgc cgtttactac catcaacact tggtcgaagt taatgctggc accggtttcg
     3241 gcttctaatt tctctaaacg aaggacttcg ccttctacca cgcggtgttg tttaccgccg
     3301 gatacaataa ctgcgtacat gttttatgct ccagagcatg tggacgtcgc gaagcactct
     3361 taggagtttt aaacccaccc aaccactgga tgaatcacaa agcttgcgcc gttttaggat
     3421 attggtaagc gacgcacagg gcgcacgcca aaccaaaggg ccgcggattc tactcaatcg
     3481 cgcccgctgg cgcaagcggg caaactgggc tgcgggtgct tttgggcagt atgtccccct
     3541 agagccggaa gcccgccacc atggattgca ggtcttttgc ggtttgatcc aacccctggg
     3601 tgaccgcgcc ggttttgcgg gcgtgggcgt gggcattttc cgccagagag gccatgctca
     3661 ccacattgcg attgatttca tcggcggcgt ggctctgctc ctgggccgcc gctgcaattt
     3721 ggtgggtcat gtcgcgaatc aaaccaatgg cgccggagat gtcgctcatg gtgcgaccgg
     3781 cgttctccgc ctgggccact gaatcgcggg ttttgccggt gctttgcagc atggcctggg
     3841 ccgctgagga ggcgccatct tgcagggctt tgataatttg ctggatctcc tcggtggatt
     3901 cttgggtgcg cttagccagc gcccgcactt catctgccac caccgcaaaa ccgcgcccct
     3961 gctcgccagc gcgggccgcc tcaatggcgg cgttgagggc tagcaggttg gtctgctcgg
     4021 cgatgccttt aatcacatcc aacactgccc ccacgctggt ggattcctct ttgagccggt
     4081 tcatctgctc ggaagtctgc gccacctcgt ccgctacccg ggtgatggcg ctgatggcac
     4141 cggacataat ctggttgccc tcgtgcaaac gggtctcggc cagcaccgtg gcatccgccg
     4201 cctgggaggc atttttggcc acttcgctga cagtggagac catttcgttg atggcggtgg
     4261 caatctgccc ggcatagcct tcggttttag ccgtatcccg atccaggccg atagaggatt
     4321 gattgagctg ttcattcacc tgggtcaata ccatagcagt actgcgcacc ccgccgatca
     4381 tgctgcagag ggcgccgcgc agtttttcca cgttgttaaa cagcgcaccc aattcatcct
     4441 cccgcaccgc cgccaagctg cgggtgtagt cgccctccgc caagcggcga atatggtcaa
     4501 tcacctgggc gagggggttt aacacctggg atttcacact caccaccaca gcaaacacca
     4561 ccagggcggt gagcaccaat tccaccacca gggtgcggcc atatatggcg ccagcggcgg
     4621 cgctcaaagc ctgcgtgtct ttttccatgg atgcgttgag catatcggcc agtgcatcaa
     4681 ttttttgcga caactcgcga tcgatcccct tgaccgcact gtcgcccacc ttggcatcga
     4741 agcccgcctg ctcaaaggct cggcggccct cttcatactt gggcagtaag gcggagtgca
     4801 aggtgctgag gttgcgcaga tccttagcca atgggtcatc atccaacagc ttaacggcct
     4861 cctccacctt ggcctcgaat tttttgtgct gttcggtaaa tgcctgccag tatttttgcc
     4921 cgtcagaagg attggagccg cgcagcaaaa cgtttttcca ctcctgcact tgcgccttaa
     4981 acaccacatt gaggtgatca acactctcgt tgacctctga tttatggtgc agaacatatt
     5041 ccatgcgctt gataacgccc tcttgttcta caccccccca aatccccacc agggccagca
     5101 ccaggcaacc tgc
//
LOCUS       DAOQGS010000290         5105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8471_length_5105_cov_3.865347, whole genome shotgun sequence.
ACCESSION   DAOQGS010000290 DAOQGS010000000
VERSION     DAOQGS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_5105_cov_3.865347"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="PKZ52_11285"
     CDS             complement(<1..1860)
                     /locus_tag="PKZ52_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNG60400.1"
                     /translation="MLINNCKNRSPAAHLRWFLGAFSVYCITAMPLGFAAIEDDSDGD
                     GILDVVDNCTKVANSNQRDTDGDGFGNICDGDLDNNGIVNSLDYSAFKARLFSRDANA
                     DLNGDGIVNSIDFTLFKRLLLKAPGPGNPSEATFVDAPPPVTDVQIFKLEQPVQERNA
                     ALAASFSQVTGLKPVISFNFENSTVGLNDLGLEPDAKAGDGIYSGYLNHNFEAEDKTA
                     ANFQQRLSSSETKQPVLFSGRDVIGRSAQPFVEPAIQADTRALPIKTSVSASLVLQPL
                     DLVPILPVAHDAARSLMITHRNVVADPTRTFDPCDVDGDNNQGNVNGAWSFKTLMTNM
                     ANTAVSGISAQEFTHNWINKWMSNQSVNGFTINARPNFQNFFPGWNPANPATLNMNRL
                     PFRLLAIVNRMDLAVSSPYNGVGGQPGETRLVFGLIDLSSCPAHNISPTREMTVIFEY
                     GDPSSACTSLKNRANDWIHLSTLPIGTAAYNTALQALTDDVTLANSAPGKPNKSAINQ
                     VRTNEIALSFPWQLREFTLKSGAVSSNLLPATIKQTPDPGLFRFGSGVTADFMEEFAD
                     AISCERHKVPESYQSQHFLGSHADYAFGTIWHAPAAVTPGPDFCTETPFTGTPT"
     gene            complement(1873..2445)
                     /locus_tag="PKZ52_11290"
     CDS             complement(1873..2445)
                     /locus_tag="PKZ52_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNG60401.1"
                     /translation="MYKKLSVAILTGALFSLPVHAANVVIDPSNSTHNQGDVFSVDVL
                     GKGFTTALDAGGVNLSFDSTILAIADSSKLPLGVSNSVQFKSPWTLTFAPSISDGSLN
                     DALFFADSAPSGNFSIFTVWFEATAPGTSPLTLTESTLNPFAGAGGGLAVSLTNAQVS
                     VVPLPPTAWLFASGLVLLVTQRKIRAQRAA"
     gene            complement(3410..4426)
                     /locus_tag="PKZ52_11295"
     CDS             complement(3410..4426)
                     /locus_tag="PKZ52_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HNG60402.1"
                     /translation="MNFLVRHLNTPAFAYRLLCTGLLSCAAANVSADALTPTTLKIKY
                     EITWDDTFFSPNAAQFNLATTSETWDVTFKVGNESFVVEGSGTPVPASLNTVEGADLA
                     SSFNIVSTSEIPSSSDAARFDTLTSRDVFKFESNLLNSNTEFFSITDALFGRYVPQTG
                     NQLITWSKTRQITSPQILAGTIARTTAGIKAALNNRNYTYQVTDSFLNNVLQTAYNKT
                     GTARVVSLTEVTDTGPTPAPASCKYVVYSDWGTGFNGAVIIKNKTNQPITGWQLNLTL
                     LGGGSISQIWGARILGSAPSYSASFLSWNETIWPNQEINFGFNGVKTAGQSAAASVSG
                     GICQ"
BASE COUNT         1294 a         1075 c         1333 g         1403 t
ORIGIN      
        1 ggttggtgtg cctgtgaagg gggtttccgt gcagaaatct gggccgggtg ttaccgctgc
       61 tggtgcgtgc caaattgtgc caaaggcata atcagcgtgg gaacccagaa aatgttggct
      121 ttgatatgat tcgggcactt tatggcgctc acaggaaatc gcatctgcaa attcctccat
      181 aaaatctgca gtcacgccag agccaaaccg aaataatcct gggtcagggg tttgtttaat
      241 agtggcgggc aataaattag aactgaccgc gccggacttg agggtaaatt ctcgcaattg
      301 ccacggaaac gacagagcaa tttcgttggt gcgcacttgg ttgatcgcgc ttttattggg
      361 tttgccaggt gcactattag caagggttac gtcgtcggtt aaggcttgta atgcagtgtt
      421 gtacgccgct gtgccaattg gtagagtgga taggtgtatc caatcattag cgcgattttt
      481 tagcgatgtg caggcggagc tgggatcgcc atattcaaaa atcaccgtca tttcgcgggt
      541 tggcgaaata ttgtgggcgg ggcagctact taaatcgatt aacccaaata ctaaacgggt
      601 ttctcctggt tgcccaccca cgccgttgta aggtgatgaa acagcaagat ccattcgatt
      661 aactatggct aataatcgaa agggaaggcg attcatattc aacgtggccg ggtttgccgg
      721 attccagccg gggaaaaagt tttggaaatt gggccgcgca tttattgtga aaccgttgac
      781 gctttggttg ctcatccatt tattgatcca attatgggta aattcctgcg cggaaattcc
      841 gctcaccgcc gtgttggcca tattggtcat gagggtttta aatgaccagg cgccgttgac
      901 gttgccctga ttattgtcgc catccacatc acagggatca aatgtgcgcg ttgggtctgc
      961 cactacattg cggtgtgtga tcattaaact gcgggcggca tcgtgggcga ccggtaaaat
     1021 aggcacaaga tccagtggct gtaaaacgag actggccgac actgatgttt taatcgggag
     1081 tgcgcgagta tccgcttgta ttgcgggctc aacaaaaggc tgagcgctgc gcccaattac
     1141 atcccgaccg gaaaataaca ccggttgttt ggtttcactg ctgcttaagc gctgttgaaa
     1201 attggcagct gttttatctt ctgcttcaaa attatggttt aaataacccg agtagatgcc
     1261 atcgccagct tttgcgtctg gctctaatcc gaggtcgttc agccccacag tgctattttc
     1321 aaaattaaat gaaatgacgg gtttaagccc agttacttgg ctaaatgatg ccgccaaggc
     1381 cgcattgcgc tcctgtaccg gttgttcgag tttaaaaatt tgcacatcgg taactggtgg
     1441 tggtgcatca acaaaggttg cttcactggg gttgccaggc ccaggtgctt tgagtaatag
     1501 acgtttaaaa agagtgaaat cgatggagtt gacaatacca tcgccattga gatcggcatt
     1561 ggcatcgcgg ctaaagagtc gagctttaaa tgctgaataa tcgagggaat tgacgatgcc
     1621 attattatct aaatcgccat cgcagatgtt gccaaagccg tccccatcgg tatcccgctg
     1681 gttactgttg gcaaccttgg tgcaattatc gaccacatcc aaaatgccat cgccatcact
     1741 atcgtcttca atggccgcaa aacccagcgg cattgccgta atacaataaa cactaaacgc
     1801 gcccaggaac caacgcaggt gcgcggctgg gctgcgattt ttgcagttat taattagcat
     1861 aacagcctcc tattaagcgg cgcgttgggc acgaatttta cgctgagtta ccagtaacac
     1921 taagcctgag gcgaataacc aggctgtcgg tggcaaaggc acaacactga cttgtgcatt
     1981 ggttagactc accgcaagcc cgccgcctgc accggcaaaa ggattgagcg tggattcggt
     2041 tagggttaag gggctggtgc ctggcgccgt agcttcaaac caaaccgtga aaattgaaaa
     2101 atttccgctt ggagcgctat cggcaaaaaa taatgcgtca tttaatgatc cgtcgctaat
     2161 gctaggtgcg aatgttaagg tccatgggct tttaaactgc acactattgc tcacacccag
     2221 cggcaattta ctgctatcgg cgatggctaa aatagtgcta tcaaaagata aattaacgcc
     2281 accggcatca agagcggtgg taaaaccttt ccctaaaaca tccacagaaa aaacatcgcc
     2341 ttgattgtgg gtggaattgc tggggtctat gacaacattg gcggcatgaa ctggcagcga
     2401 gaatagggcg cctgtaagga ttgcaacaga taatttctta tacataacgg aagcctcatt
     2461 tgaattaatc ggaaagggta aggtgatgaa tgcccccaaa agccgctaat aacacataaa
     2521 tccataatgg gcgcccacag cacaggtgcc ggtgcctgaa tgtgttgtcc cttttgttaa
     2581 atcctagata agcatcccaa tgtctgtcag atgcctaagg ctataaatgt gttatctaaa
     2641 tggagggtat cgcacagatc gttgacgata ggtgtgcgag gaaaacaata taacatgcta
     2701 atacagcgat attaatttga tagcttgtga aataataccg tcaagtgctg ttgcatatga
     2761 gccattgggg ctaaaccact ggtggttggg cggtgcagga ttgctgtaag aatcttttac
     2821 tattattggt gggttcttaa tggcgattta gtcttggtga gcgcaggcag ccctcccttt
     2881 gggtggttgg cagatagtcg atttgtgaat gcggcgttgc acattgatga caaaaattac
     2941 aagcgttgcg aacgcaggct aaaagtgtgt ttaaaaattc gtaactattc acgacctttt
     3001 agttcgatac ctttttagcg taactggacg ttattctcgc gccgactgga aaccatcaag
     3061 tgaattcttg agtgctgcga gcccgcagtg atgaatacag gttattgcag ccacgccgcg
     3121 aatatatcca tgtgggtccg tcaaaaaatc cctctttttg ctgtctgaaa ttatctgtag
     3181 tcaccgctgc tgcgccgcga attaattatg ctccctggct gattttcctt aatttgccgc
     3241 ccgaatggag agggcaatag ggtacgctga atagttatta accctgcggc tttttatgcc
     3301 gggttaatac aaaaactctt ggagagagcg tttaggctag ggggaggtat ttttaaatgg
     3361 agcttgtcaa agcttttgaa atataggagc acacttccct gtgtatatct tactggcaga
     3421 ttccaccgct tacactggca gcagcgcttt ggccggcagt tttaacgcca ttaaaaccaa
     3481 agttgatttc ttggtttggc cagatggttt cattccagga taagaagctt gcgctgtaac
     3541 taggtgcgga gcccaaaatg cgcgcacccc agatttggct gatgctaccg ccacctaata
     3601 gggttaggtt aagttgccag ccggtaatgg gttggttggt tttatttttg ataatgactg
     3661 cgccgttaaa acctgtgccc cagtcggaat agactacgta tttacagctg gccggggcag
     3721 gagtggggcc ggtgtcggtg acttcggtta atgacaccac ccgcgctgta cctgttttat
     3781 tgtaggcggt ttgcaatacg ttatttaaaa aactatccgt gacttgatag gtgtaattgc
     3841 ggttattgag ggcagcttta ataccggctg tggtgcgtgc gatagtgcca gccaaaattt
     3901 gcgggctggt aatttggcgg gttttagacc aggttattaa ttggttgccg gtttgtggta
     3961 catagcggcc aaataaggcg tcagtaatac tgaagaattc ggtgttgcta tttaataaat
     4021 tggattcaaa tttaaacacg tcgcggctgg tgagtgtatc aaagcgtgca gcgtcgctgc
     4081 tggaggggat ttcactggtg gatacaatat taaatgagct ggccaggtca gcaccttcga
     4141 ctgtgttgag gctagcaggt acaggtgtgc cggagccttc caccacaaag gattcattgc
     4201 ctactttaaa ggttacatcc caggtttcgc tggtggttgc taaattaaat tgtgcggcat
     4261 tggggctaaa aaaagtatcg tcccaggtaa tttcgtattt tatttttagt gtagtggggg
     4321 taagtgcatc ggcgctaaca tttgcggcag cacagcttaa taatcctgtg cagagtaatc
     4381 ggtaagcgaa ggctggtgtg tttaagtggc gcactaaaaa attcatattt atatcctgtt
     4441 aatgtacatc agaaaaacga gtgcacctaa tggacgcaaa atgccgtcat cacgcctggg
     4501 taatggccga tattttcggc cattagagca actccttgag tgggcgaaca attgacgcca
     4561 ataaattggc ttcccttaaa ttagcccggc cttcgcattg ctatatctgt gccagtaacc
     4621 gtgcaaattc agttgttgat tttaatgacg aggccggtag ctttattttg caggtattta
     4681 gcatgatggt attttttaag ctgaggttga acacctgccc aacaccgttg ggcgataatt
     4741 gttgtaaata gattcggcaa atttatggcg atcttttaat gctgtcgcgt cgctaccaaa
     4801 gttttttacg cagagaaata agcaacagta gcgcgcggac gggattatta ggcggatagg
     4861 tttgtatctg cctgaaaaat tacttgataa ttttttcgat tgtaactttg acttttaccc
     4921 gttcgttcag gattagggga gtattccgcc tctgacgagt gcgagcacat cgattagtgt
     4981 tttattcgct taggccagtg gcgggtgagt ctgcggcagg tggttgcaaa ggccgcatgg
     5041 gctagggcgg tttattttgt tatggtaggc gctgccactg gaggatccta tgctcgataa
     5101 acgcc
//
LOCUS       DAOQGS010000291         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8473_length_5104_cov_3.259655, whole genome shotgun sequence.
ACCESSION   DAOQGS010000291 DAOQGS010000000
VERSION     DAOQGS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8473_length_5104_cov_3.259655"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1460
                     /gene="sbcB"
                     /locus_tag="PKZ52_11300"
     CDS             18..1460
                     /gene="sbcB"
                     /locus_tag="PKZ52_11300"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HNG60403.1"
                     /translation="MHTLYWHDYETWGIDPSIDRPCQFAGVRTDEDLNIIEEPLRLYC
                     QPAIDILPNPEACLVTGIAPQKAQQEGLIEAEFIAAIHGALAVPGTCGVGYNSLRFDD
                     EVTRFTLYRNFYDPYEREWKNGNSRWDILDMLRLVHALKPDTLNWPEWEGRVSFKLEL
                     LTKANGIEHGAAHDAYSDVAATIALAKLIKTRQPKLYDYCYNLRLKHNVSAHIDLIQK
                     KPLLHISSKFPIEQGNAALVMPLCYHPVNKNAVLVVNLAQDPQQWIHLSAEELAARLF
                     TTQTEDSPPRPSIKAIHLNKSPIVATPKLLDAQTQARLKIDKQQCEIHWQQLIKLDLT
                     EKLQAAFSINTFAPREDPERKLYDGFLPNGDMQLAKALRTAKPEEITAEQFCFSDKRL
                     QAMLLRYKARNFPHALTEREQQQWFEFCHGRLSDGEPGILSCDQLKMRIRTIDETQAL
                     TKEQKIVLQQLYRYVEDVAVTFRLKPKCCW"
     gene            1494..2510
                     /locus_tag="PKZ52_11305"
     CDS             1494..2510
                     /locus_tag="PKZ52_11305"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNG60404.1"
                     /translation="MSLAGKHVLSVDQFERADIEHIFTVADSMAPFALRQKVTRVLEG
                     AILGNMFFEPSTRTRVSFGCAFNLLGGNVRETTGLESTSLTKGESLYDTARVLSGYSD
                     IICMRHPTAGSVAEFAAASRVPVVNGGDGANEHPSQALLDLYTIAKELQHFGRSLDGL
                     RIAMIGDLKHGRTVHSLCRLLGLIANVQVTLVSPQELAMPELYVELLRNAGHQVRIST
                     DLTQSIGSVDIIYSTRIQEERFSTKAEADLYRGRFRLNQAIYTQYCEPNTVIMHPLPR
                     DSRAEANELDNDLNQNPNLAIFRQADNGILVRMALFALILDVADQVEKFARPVNWYHR
                     RSGV"
     gene            complement(2609..3559)
                     /locus_tag="PKZ52_11310"
     CDS             complement(2609..3559)
                     /locus_tag="PKZ52_11310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG60405.1"
                     /translation="MPKPAQPKPSIVHVNATAAIDPVAPDCESVLREQRFRLAHELQS
                     SLDLRDTLSHFFTRARQVVKFSSLNFINDAKRLNVTLGRGQKHRVEYNLSGDNYQLGL
                     LSFSRDSPFSETELQGLEILSGILFYPLRNALLYREALDNSLRDTLTQVGNRAAMEMA
                     LKRELELAKRNNLPLSLLVIDIDHFKRVNDSCGHSLGDQVLTHIAAIIKDSLRQTDQT
                     FRFGGEEFVVILCDTQASAAQMVANRIRLNVHASTLFAARDIHSSVSIGLAYLAPDDD
                     RDSLFSRADSALYLAKQMGRNCVVNETELGATNLCQEALM"
     gene            complement(3841..4209)
                     /locus_tag="PKZ52_11315"
     CDS             complement(3841..4209)
                     /locus_tag="PKZ52_11315"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNG60406.1"
                     /translation="MKPGNTGVSRLIHALTYSKQGLIFAYKNEAAFRQEIWLGVILIP
                     VALLLPIGLIHKVTLIGSLILVAVVELLNSAIEAVVDRIGPEHNELAGRAKDMGSAAV
                     MLTLVVVVLLWSAELYSLTR"
     gene            complement(4278..4379)
                     /locus_tag="PKZ52_11320"
                     /pseudo
     CDS             complement(4278..4379)
                     /locus_tag="PKZ52_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014070492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
BASE COUNT         1254 a         1236 c         1293 g         1321 t
ORIGIN      
        1 aaatttaaag gccccctatg cacactctct actggcacga ttacgaaacc tggggcatag
       61 accctagtat tgataggcct tgccaattcg caggtgtgcg caccgatgaa gacctcaata
      121 ttatcgaaga gccactcagg ctctactgcc agcccgcaat agacattttg cccaatcctg
      181 aagcctgttt ggtaacgggc atagcgccgc aaaaagctca gcaagaaggc cttatagaag
      241 ccgaatttat cgcggcaatt cacggtgctt tagcagttcc tggcacctgt ggggtcggct
      301 acaacagcct gcgctttgat gacgaagtca cccgttttac cctctaccgc aatttttatg
      361 acccctatga gcgggaatgg aaaaatggca atagccgttg ggatattctc gatatgctta
      421 ggctggttca cgcgctcaaa cccgacaccc taaattggcc cgaatgggag gggcgggtga
      481 gttttaaatt agaattgctc actaaagcca atggtataga acacggtgcc gcccatgacg
      541 cctattccga tgtggccgca accattgcgt tggccaagtt gattaaaacc cgccagccaa
      601 aactatacga ctattgttat aacctgcgtt tgaaacataa tgtcagtgcg catattgatc
      661 ttatccaaaa aaaaccattg ctgcatatca gctctaaatt tccgattgag cagggcaatg
      721 ccgccttggt aatgccgctg tgttatcacc ctgtcaataa aaatgcggtg ttggtagtta
      781 atttagcgca agatccgcag cagtggattc atttaagtgc agaagaactt gctgcacgtt
      841 tatttactac acaaaccgaa gacagtccgc cacggccgag tattaaagcc attcacctga
      901 ataaatcgcc tattgttgct acgcctaagt tgcttgatgc ccaaacacag gcgcgattaa
      961 agattgacaa acaacagtgt gaaatccatt ggcagcaatt gataaaactg gatttaactg
     1021 aaaagctgca ggcggcattt agcattaata catttgcacc gcgcgaagat cccgagcgca
     1081 agttgtacga tggttttttg ccgaatggtg atatgcagct tgccaaagcc ctgcgcaccg
     1141 caaagcccga agaaattacg gcggagcaat tttgttttag tgataaacgc ctgcaggcga
     1201 tgttgctgcg ctataaggcc cgcaattttc cccacgcgct caccgagcga gaacagcagc
     1261 aatggtttga gttttgtcac gggcggttat cagatgggga gccgggaatt ttatcctgtg
     1321 atcaacttaa aatgcgcatt cgaacaattg atgaaacaca ggcgctcaca aaagagcaaa
     1381 aaattgtact gcagcaactc tatcgttatg tagaggatgt agctgtgacc tttaggttaa
     1441 aacctaaatg ttgttggtaa gacgttttaa ttaaatatta aatggggact caaatgtcat
     1501 tagcgggtaa acacgtattg tcggttgacc aattcgagcg cgccgacatc gaacacattt
     1561 ttaccgtggc cgattctatg gcgccgtttg cgctgcgcca aaaagtaacc cgagttttag
     1621 agggtgctat tttgggcaat atgttttttg aacccagtac ccgcacccgc gtaagcttcg
     1681 gatgtgcctt taatttattg ggtggcaatg tgcgtgaaac cacgggcctt gaaagcacct
     1741 cgttgaccaa gggagaatct ttatacgata cggcccgggt gttatccggc tatagcgata
     1801 ttatctgtat gcgccaccca acggcgggtt cagtggcgga atttgccgcc gccagccgag
     1861 tgccggtggt aaacggtggg gatggtgcca atgagcaccc tagccaagca ttattagact
     1921 tatacaccat cgccaaagaa ttacagcatt ttggccgcag cttagacggg ttacgcatcg
     1981 cgatgattgg cgatttaaaa cacggtcgaa cagtgcattc actgtgtcgc ctgttgggtt
     2041 taattgctaa tgtacaagta actctggttt cccctcagga gctggctatg cctgagttat
     2101 atgttgaact gctgagaaac gccggtcatc aggtgcgtat ttcaaccgat ttaacccaga
     2161 gtattggcag tgtcgatatt atttattcca cccgtattca agaagagcgt ttttctacta
     2221 aggcagaagc tgatctgtat cgcgggcggt ttcgcttgaa ccaggctatt tatactcaat
     2281 actgcgaacc gaacactgtg attatgcatc cgctgccccg cgattctcgc gctgaggcaa
     2341 acgaattgga taatgactta aatcagaacc ctaatctcgc tattttccgt caggccgata
     2401 atggcatttt agtgcgtatg gcactgtttg ctttaatttt ggatgtggcc gatcaggtag
     2461 aaaaattcgc ccggccggtt aattggtatc accggcgttc gggcgtctag gtgcttaaat
     2521 tattgctttt tatgcgataa aactggtggg gtgggtcagc acaacaatgt gttgaggcag
     2581 taggattttt tatactccac tggttgaatt acatcaatgc ttcctggcac aggttggttg
     2641 cacccagttc ggtttcgttg acaacacaat tgcgccccat ttgtttggcc aggtataaag
     2701 cgctatcggc gcggctaaat aaactgtccc gatcatcatc cggtgccaga taggcaagcc
     2761 caatactgac ggacgaatga atatcccgcg ctgcaaataa agtggatgcg tgtacgttta
     2821 gccgtatgcg gttggccacc atttgtgcgg cgctcgcttg ggtatcacat aaaataacca
     2881 caaattcctc accgccgaag cgaaaggttt gatcggtttg gcgtaacgag tctttaatga
     2941 tggcggcaat atgggttaat acttgatccc ccaggctgtg gccgcagctg tcgtttacgc
     3001 gcttaaagtg gtctatatca atgacgagta aacttaaggg caaattattg cgtttagcca
     3061 gttccaattc tctttttaga gccatttcca tggccgctcg attgcctact tgggttagtg
     3121 tatcgcgcag ggaattatcc agtgcttcgc gatagagtag ggcattgcgc agtggataaa
     3181 acaaaatgcc tgataatatt tccagcccct gtaattccgt ttctgaaaag ggactgtccc
     3241 gcgagaagct caacaggcct aactgataat tatcaccgga taaattgtat tccactcggt
     3301 gtttttgtcc tctaccgagg gtaacattca gtcgttttgc atcgttgata aaatttaagc
     3361 tgctaaattt caccacttgc cgggcgcggg taaaaaaatg gcttaatgta tcgcgcaaat
     3421 caagggagct ttgcaattca tgggctaagc ggaagcgctg ttcgcgcaat acggattcac
     3481 aatctggcgc taccgggtct attgccgctg ttgcattaac gtgaactata gacggtttag
     3541 gttgcgcggg cttgggcata tggacatcct gatgacaact tgacggttaa gtgatcaaaa
     3601 gcgaatacta tgccagctta tagttttaga attttttcat ttaaaatcat atgtttagcc
     3661 agataatctg cgctggttgg cacctgctgg tcagaatttt gactgtgttg tacgccttgg
     3721 ttatccgctg taatgcttag caccgccaga ccttgggata ttctgattaa gtatgccgcc
     3781 tgcgtttttc ccatggaatt taaataacag ttgctgccaa ctgcggcgag aaggtcgaaa
     3841 ttagcgagtt aagctgtaga gttcagccga ccaaagtaac acaaccacaa cgagcgtgag
     3901 catcacggct gctgaaccca tatctttggc tcggcccgct aattcattgt gttctgggcc
     3961 aattctatcc actaccgcct ctatggctga atttaacagt tcaactactg ccaccaagat
     4021 caggctgccg atcaaagtaa ctttgtggat tagcccaata ggtagcagca gggcaactgg
     4081 aattaaaatc accccaagcc atatttcttg gcggaacgcc gcttcgtttt tatacgcgaa
     4141 aattaagcct tgtttagagt aagttaacgc atgaatgagg cgggacaccc cggtattgcc
     4201 aggtttcata atgtttaatc gcgcgcttaa agtgatggga aataatggaa cacaaatgac
     4261 ggaagggtat gaatgctcta accgcctgcc aatttaacct taaagccttt ttgttccaga
     4321 taggatttaa ttttttcgcg gtggtcgccc tgaatttcca gcacgtcgtc cttgagcgac
     4381 acgacctgga cccgcacgtc ggggttgccg aactgatcct ggaactccac gaaggcctcc
     4441 ttgaggttgg ccacgtagct ctccagcgag atgtcgccgt gggtcaccag gcggaccaag
     4501 aagaggtcgc cgaggtgagc gtagctctgg ctggtatagt actcatagac cttggtcgcc
     4561 ttgtaggcct cgcgcaccgc cgcccggaac gtgcgatccg cggccaggat gtcatcgttc
     4621 ttgtagatgc gggcgttggg atcgttgcgc gccgcctcta cgtcgatggc cagctgctca
     4681 gactcccgct gatcttggat gagccgggtg gcgcggtttc gctccttgcc caccaccgag
     4741 accgcgacct tgagctcgtc ggccttgccg aggatctcga ggtcgtggac caccatctcc
     4801 agccagaggt tggagatgag ggcccgagag tcgaccgtgg cgtagtcgca catggcctcg
     4861 gtctgcacgt tggcgatgtc gttttggagc ttctcccgct ccacctccag ggccccgatg
     4921 gtgatgtcgg aggccacgat gaagggctcg gtcgccgccg cgatggccac cgcagcgccg
     4981 aaggtctcga tggccgcggc ggcggtctcc cgggctcgct cggcgaagcc cttgatggcc
     5041 tcggccgcct ggatcccgcg gaacatggag atgctcacct cctgctgatc cttgatgtag
     5101 ttca
//
LOCUS       DAOQGS010000292         5059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8581_length_5059_cov_3.977618, whole genome shotgun sequence.
ACCESSION   DAOQGS010000292 DAOQGS010000000
VERSION     DAOQGS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8581_length_5059_cov_3.977618"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1194
                     /locus_tag="PKZ52_11325"
     CDS             271..1194
                     /locus_tag="PKZ52_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60407.1"
                     /translation="MATDLSLINSTPPRDYGRPHIFPGVPLEVEGDWAGVHGLAAPYR
                     LADLGQGPVYAPRAQYKGQIVIWDSNFDCAQLPKHIQLRQGDINVHAGNFALADNSLR
                     ILNCRLVGPALINAEGAIQLHMQPIFELDYQAEYFPAQVAELRVVTSQRFLNLHNGKT
                     TLLTHTNETQGPAVVLGEMNTLFDDTEYLTQIFTPVLWQEHKQLRADMPIYQTIINEC
                     DGVPVTSLTVLEQYTSYFLQRYYAPTKIGCWVPAAPPITWGLSIRVGKNGEDEWAIVR
                     RKLIMPTPGHEGLQLPHWSGSLRTTPHGYAT"
     gene            1191..2294
                     /locus_tag="PKZ52_11330"
     CDS             1191..2294
                     /locus_tag="PKZ52_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG60408.1"
                     /translation="MSQPLPVAIIGGGLCGVYLAWLLQKQGIQFQLFESAVRMGGRIL
                     SQNGFDLGPTWYWPHEHRQLDQLLQQLTINTLPQWQSGLFLYQTSRELPAQVFKDPAG
                     YGQAKRISGGAQVLIQTLLNAIDPAAINFGHHLVQINQHPAGVELVLSECGTPVHTIA
                     AQAVLAMPPRLIQHTIKFNPPLDKPLRTIMGATPTWMAGQAKVIFEFAEPFWRAQGLS
                     GNAIAQYPGAILGELFDACSQNQFALGAFLALPPHLRINSRANWSTDLEGLMRAQLIH
                     LFGPAAAKVQAIWFKDWFCEALLSCSEDAVPPSQHPSYNHPWLQLDHWSERLYFAGTE
                     TARQHGGYMEGALVAARRVFQDLTGIATINYPP"
     gene            2361..2897
                     /locus_tag="PKZ52_11335"
     CDS             2361..2897
                     /locus_tag="PKZ52_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNG60409.1"
                     /translation="MNNSALNENNHWVIWNGAYGIVDTVTIERTQRCDNTTLAWLDEP
                     YDMVGPFNFDALQMYGAINYQACLIMSAEKWQQDQVHLRRESIRLKREAMVRAYEQQA
                     RFNQRRHMHNNSSAAINEKALRQTLNLPAEGTLTKSHINAAYRRLAQKTHPDHGGNQE
                     EFVRVTQARDRLLEIMAT"
     gene            3836..4546
                     /locus_tag="PKZ52_11340"
     CDS             3836..4546
                     /locus_tag="PKZ52_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HNG60410.1"
                     /translation="MATVLKNGSSGDAVKRLQEQLNAVLKLNPPLTTDGKFGTKTERA
                     VMAFQAKNKLGVDGIAGPKTQAALGAKLDGKPVAAPPPSPAASPSHTSGAAWFEIAKK
                     EIGVAEKPGSAANGRILDYFKATTLTTKSDETAWCAAFVNWCLDKAEKTGTKSAAAAS
                     FVNWGTNAGAKTGAVTVIHNPSMANSSLSRSGNHVGFLVEETSTHYVLLGGNQSDQVK
                     ESRFPKSSWKLKGHRWPK"
     gene            4560..5000
                     /locus_tag="PKZ52_11345"
     CDS             4560..5000
                     /locus_tag="PKZ52_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60411.1"
                     /translation="MSFIRKFSFFWLVTSLLLGCAIAKEPAPSDFAIYWANFHKAFID
                     KDFKKIQDLSVTSILLRGAVDGIKPKKLSAQDFDKTFSAILATEVSEYQGEKRVVQST
                     YEKIKSISTPKIEQDPARQRVEGFVFKKENGQWKLAEIYWDELE"
BASE COUNT         1383 a         1219 c         1231 g         1226 t
ORIGIN      
        1 agaaatcacc tgcaccactt atcatcttca ttcctcgctg ttattcacca ccaaaggcgt
       61 aaacacgcaa gcggttagga atgtcagata tacaacaggt aaagtcgcct tatgcgttgg
      121 gcgtagccct aggattagct cgtcaatttg gctattagct atttgccgtt gtttgctatt
      181 aacccggcac taaatgccgc cggagtaata aatggcacag tttctgatgg tccccttagg
      241 tttacgtctt aaatcctaca aacccaagct atggccacag atctaagcct aattaacagc
      301 accccaccgc gagattacgg gcgcccgcat atattcccag gtgtacccct tgaggttgag
      361 ggcgattggg cgggtgtgca tggccttgcc gccccttaca ggctcgcaga tttaggccaa
      421 gggccagtgt atgctccgag ggcgcaatac aaggggcaga ttgtgatttg ggatagcaac
      481 ttcgactgcg cccagctgcc caaacacatt caattgcggc agggcgatat taatgtgcac
      541 gcaggtaatt ttgcccttgc cgataacagt ttgcgcattc ttaactgccg ccttgtgggg
      601 cctgcattaa taaatgcgga aggcgcaata caactacaca tgcaacccat ttttgaattg
      661 gactaccaag cggaatattt tcctgctcag gttgcagaac tgcgcgttgt aacaagccag
      721 cgctttttaa atctgcacaa tggtaaaacc acactattaa cccataccaa tgagacccaa
      781 ggcccagcag tggttttggg ggaaatgaat accctgtttg acgatactga atatttaacc
      841 caaatattta ccccagtgtt atggcaagaa cataaacagt tgcgcgccga tatgcctatt
      901 taccaaacaa ttattaatga atgtgacggc gtcccggtga ctagccttac ggtactggag
      961 caatacacca gttattttct acagcgttat tatgcgccca ccaaaatagg ctgctgggta
     1021 cccgccgcac cacccataac ctggggtttg agtattcgcg tgggcaaaaa tggcgaggat
     1081 gaatgggcta ttgtgcggcg caaattaatc atgcccaccc caggccatga gggcctgcag
     1141 ttgccccact ggagcggcag cctacgcaca acaccacacg gctatgcaac atgagccaac
     1201 ccctgcccgt cgccattatt ggcggaggcc tgtgcggggt ttatcttgct tggttgctgc
     1261 aaaagcaagg tattcaattt cagttatttg aatcggctgt gcgcatgggt gggcggattc
     1321 tcagccaaaa tggctttgat ttaggcccca cctggtattg gccccacgag catcggcagt
     1381 tggatcaatt gctacagcaa ttaactatta ataccctgcc ccaatggcaa agtggtctgt
     1441 ttttatatca aaccagccgc gagttgcccg cccaagtatt taaagaccca gcgggttatg
     1501 gccaggccaa acgcatttcc ggcggcgcgc aggtactgat tcaaacatta ctaaatgcca
     1561 ttgacccggc ggcaataaat tttggccatc atttagtcca gattaatcaa cacccggccg
     1621 gagttgaact ggtgttaagc gagtgcggca cgccagtgca cacaatcgca gcccaagctg
     1681 tattggcaat gccaccgcgc cttattcaac acaccataaa atttaacccg cctctagata
     1741 agccattgcg cactatcatg ggcgcaacac cgacttggat ggccgggcag gcaaaagtga
     1801 tttttgaatt tgccgagcct ttttggcgcg cgcagggatt atccggcaac gccatagccc
     1861 agtatccagg ggccattttg ggtgaactct ttgatgcctg tagccaaaat caatttgcat
     1921 taggggcatt tttggcgctg ccgccacacc tgcgcattaa tagtcgggcc aattggagca
     1981 ccgatttaga aggtttaatg cgcgctcaat taattcacct atttggccct gcagcagcca
     2041 aggtgcaagc gatttggttt aaagattggt tttgcgaagc tctgctcagt tgcagcgagg
     2101 atgccgtacc gccgagccaa caccccagct acaaccaccc ctggttacaa ctggaccact
     2161 ggagcgagcg tttatatttt gccggtacag aaaccgctcg ccagcacggc ggttatatgg
     2221 agggggcatt agtcgcggcc aggcgggtat ttcaagatct aacgggcatc gcaacaatta
     2281 attacccgcc ctaaaaagcc gccgggttaa taacaatcgc cacaacacta agcgcgtgta
     2341 aaccactgga gagagcgacc gtgaataatt ccgctttaaa cgaaaacaac cactgggtga
     2401 tttggaatgg cgcctacggc atagtggata cagtaaccat tgagcgcacc cagcgctgtg
     2461 acaacaccac cctggcctgg ctggatgaac cctacgacat ggtggggcca tttaattttg
     2521 atgcactgca aatgtatgga gcgataaatt accaggcctg tttaattatg tccgccgaaa
     2581 aatggcagca ggaccaagtg cacttgcgcc gggaatcaat caggcttaag cgcgaagcta
     2641 tggtccgcgc ctatgagcag caggcccgtt ttaatcagcg ccgccatatg cacaataatt
     2701 ccagtgcagc gatcaacgaa aaagccctgc ggcaaaccct aaatttaccg gccgagggca
     2761 cactgactaa atcgcatatt aatgctgcct accggcgcct cgcacaaaaa acccatccag
     2821 accacggcgg caaccaggag gaatttgtgc gggtgacaca ggcgcgggat aggttattag
     2881 aaataatggc tacttagccg ccttccaacg ccaaactgca ctaaaaactc atcacgagtg
     2941 tggcacataa tcaatattct gcacctattg ttttaccacc cagcgtattt atagaacaca
     3001 gccggtttac tgcgctgagc ttgcccaacg agataataaa tactgctcta aatatttagg
     3061 gctcactgaa aaatgtaaac ggcgaagtgc tggctagcgc ggtttaactc agggctggtc
     3121 tcgatcccct tcaataaggc ttagaatgat taggggaaaa gaagttaaaa aaaccaaagc
     3181 gtgccgcgca tcacgttttt gacatttaac tgccaccaca gattcgaggc attgtgttca
     3241 tttaggtgcc acttgaattg cgccagaagc atttaaattt aagttcagca atataccaat
     3301 gaacagactt ggctgtacct ccattaaaag ctgcgatact catccatatg cttatctttc
     3361 aagttggcat gcacttaccg ttgaacattt ttacaagact aagccccacg gccctatctt
     3421 taggcaaggt aggattgcgc acaattttag attccaatag tattttttac gcggtttata
     3481 aatgagaata aattagcttg aattccgcat tgcaggatta aagttcttgg aattagtctg
     3541 tgctggagat tgcaagacga gcatccggtt ggaataccat ggaggttttt acggggcgca
     3601 aaatgcgctt taaataacag tatttgaact ttaaataata gtatttgaag atcgtgcttg
     3661 atttaaataa agtgcgggcc gccttagaac attgcagcct tgtcagcgga atttaactct
     3721 cttttacccg aacgcataat tacctaccgg tattgaattt tacaggccac caatcgccca
     3781 atttatgcgg tggcttgagt tcaagattta aagttagagc acatacggag tctatatggc
     3841 aactgtttta aaaaatggct catcaggcga tgcggtaaaa cgcctgcagg agcaactcaa
     3901 tgcagtctta aaactcaacc cgcctttgac cactgatggc aagttcggca ccaagaccga
     3961 gcgagcagtg atggcctttc aggccaaaaa taaattgggt gtcgatggta ttgcagggcc
     4021 taaaactcaa gccgctttag gggcaaaact tgacgggaaa cctgtggctg cgccgccgcc
     4081 ctcaccggca gcgtcacctt cacatacatc tggcgccgcc tggttcgaga tagcgaaaaa
     4141 ggaaatcggc gtggcagaaa agcccggtag tgcggcaaat ggccgtattt tggactattt
     4201 taaggccacc acgctgacaa ctaagtcaga tgaaaccgcc tggtgtgcag cttttgttaa
     4261 ttggtgtttg gataaggctg agaaaacagg caccaaaagc gctgctgcag ctagttttgt
     4321 taattggggg acaaatgcag gcgccaagac aggggcggtt accgttatcc ataacccttc
     4381 catggccaat agctcactaa gccgcagtgg caaccacgta ggattcttag tggaagaaac
     4441 ctcaactcat tatgtgctac tcggtggcaa ccagagtgat caggtgaagg agtcgcgctt
     4501 tcctaagagt tcgtggaaac ttaaaggcca tagatggccg aaataaattg gagttttaca
     4561 tgtcatttat tcgaaaattt agtttttttt ggcttgtcac atcgctactt ttgggctgcg
     4621 cgattgccaa ggagcctgcg ccatccgatt ttgcaattta ttgggcgaat tttcataagg
     4681 cgtttataga caaagacttt aagaaaattc aagacctgag tgttacttca attctgctgc
     4741 gcggcgcggt cgatggcatc aagccaaaga agttatccgc acaggatttt gataagacat
     4801 tttccgccat cctggcaacc gaggtgagtg agtatcaggg cgaaaagcgg gttgtgcaat
     4861 cgacctacga aaagattaaa tccatttcga cgcccaagat cgagcaggat ccggcgcgac
     4921 agagggttga aggattcgtt tttaaaaagg aaaatggcca gtggaagttg gcggaaattt
     4981 attgggatga actggaatag agttcaagga tgcaaggatg caaggatgca aggatgcaag
     5041 gatgcaagga tgcaaggat
//
LOCUS       DAOQGS010000293         5058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8582_length_5058_cov_4.278233, whole genome shotgun sequence.
ACCESSION   DAOQGS010000293 DAOQGS010000000
VERSION     DAOQGS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8582_length_5058_cov_4.278233"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..870
                     /locus_tag="PKZ52_11350"
     CDS             163..870
                     /locus_tag="PKZ52_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNG60412.1"
                     /translation="MWSDEFNGQGLPDNAYWSYEKGLVRNNELQYYTQARPENINMSN
                     GLLTITARKEPYENANYTAASIHTRGKVSWTYGRFEMRARIDTRAGLWPAFWLLGNGK
                     WPEGGEIDVMEYYQNKILANVAWKSPSSDQWNASWDSSTRSLSNLRLQFPDWENRFHT
                     WRMDWDNKSIRLYVDDILMNNTNLSGTKNPDGSNPFIDKPFYILLNLAIGGNNGGDPA
                     GTSFPAKYEIDYVRVYQ"
     gene            1234..3228
                     /locus_tag="PKZ52_11355"
     CDS             1234..3228
                     /locus_tag="PKZ52_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG60413.1"
                     /translation="MSVETSSPRRRVTDNRLPALEWQLVCQAFQNLPAMLMVNLSVSV
                     GGGIVLYSDGYSYVYHWLALVIVMTVVRLAVGLYFNARYTNTMVVDQKFITLWKGVFA
                     IGLFAAAGVWVAIAIATFTHQEIPPTSHYTLLIIMSAMASGATGITAPLKLIGRFYIA
                     IMIIPAAIIMLFYSNNNLIIGVLGLVFCAAMIASHASNHRLLRASLTLKLENEQLVNN
                     LQYLNSDLERRVNLRTQALKRIAHHDSLTGLPNRRGLIEWMENSLDAQKPQEAAILFL
                     DLDRFKQINDALGHDVGDKVLQTIAVRFNDLCPANCILGRWGGDEFLLITQQRPDIRQ
                     MVEHLATQLIDAATAPLEMNNEKLGLGLSVGIAYFPTDARDYKDVIQAADLTVAEVKR
                     NGRGQTLVYNDTYAETQRRRFDLSRTLAESIEHNKLHLVYQPIIDSHSGQVVALEVLC
                     RWRHPVLGDINPDEFIRLAEDTDRIIALGDWALRTACQTAQTWGDNARTIKIAINVSI
                     KQLLSNDYAGQVCATLKRLEFPFERLVLEVTETLFGEEYLDQSLGTVTQLRNLGIEVH
                     IDDFGTGYSSLSRLHQFPVAAIKIDRSFIMQLDAQSMVIIESAVMIAKRMQLKVIAEG
                     VESAAQANTLIKLGVDYLQGYYFGKPSDTPQFAPIHLESL"
     gene            3571..4248
                     /locus_tag="PKZ52_11360"
     CDS             3571..4248
                     /locus_tag="PKZ52_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNG60414.1"
                     /translation="MKTSTWLRTLFISTLAYSTVASAATSVIQLDFTGVGSRAAVKSF
                     YSGGADSDGNSGVNYGVTFSENALSLSDIDPDANFAGEPSPHNIMFFLSGPAYLSIPE
                     GFSQGFSFYYSTLDFTGQVDIYDGVDATGNVLGSLTLDALGEGPVLDRPYSNWKIAAL
                     NFTGVAKSVNFSGTVNEVGFDNITLGSIDPSIPAVPEPSSTAQLLLGVGLLALLRRRY
                     STGKNLS"
     gene            complement(4313..4822)
                     /locus_tag="PKZ52_11365"
     CDS             complement(4313..4822)
                     /locus_tag="PKZ52_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60415.1"
                     /translation="MRLHLTLFALASCLIATPVWSGEAAQPEIKLTTFRIGKLHIGMP
                     ASEVVGLPGCVFTKGKLVTSEADGGWHQTWRAPRCGLTLGFSAEDKKSPLKLFDMKVE
                     APSTLKTEKGIGIGSTDAQVRSQYLREINTEESISQQNLVLGSVYGGVIFELDKGRVN
                     SIFVGAAAE"
     gene            complement(4860..>5058)
                     /locus_tag="PKZ52_11370"
     CDS             complement(4860..>5058)
                     /locus_tag="PKZ52_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60416.1"
                     /translation="LFLVVLHKLEYFLNAKIIGTEIKSNAWELLIAAIAMESVFGLPG
                     VVMAPILYAYIKHELIRAKLI"
BASE COUNT         1354 a         1317 c         1129 g         1258 t
ORIGIN      
        1 gtggaaaatt accagcaccg gcgatggctt ttacaaaatt gaatcgcgcc tgagcagtaa
       61 gcactggatc aaattaatag cggcgtaaaa caatacacct atgccaatac cagccatcaa
      121 caatggcgtt ttgaattaat tcccgagccc cagcgcaggc tggtgtggag cgatgaattt
      181 aatggccagg gcctgccgga taacgcctac tggagttatg aaaaaggttt agtgcgcaat
      241 aacgaattac agtattacac ccaggcgcgg ccggaaaata tcaacatgag caatggcttg
      301 ttaaccatta cagcccgcaa agaaccatac gaaaatgcca attacaccgc cgccagcatt
      361 cacacccgcg gcaaagtcag ttggacgtac ggccgctttg aaatgcgcgc ccgcatcgac
      421 acccgcgcag ggctgtggcc ggcattttgg ttattaggca atggcaaatg gccggagggc
      481 ggcgaaatcg atgtgatgga atattatcaa aataaaattc ttgccaacgt cgcctggaaa
      541 agccccagct ctgatcaatg gaatgccagc tgggattcct ccacccgctc actgagcaat
      601 ttgcgcttgc aattccccga ttgggaaaac cgctttcaca cctggcggat ggattgggac
      661 aataaatcca tccgcctcta tgtggacgat attttaatga ataacacgaa tttaagcggc
      721 actaaaaacc ccgatggtag caacccattt attgataaac cgttttacat tcttttaaat
      781 ttggccattg gcggcaacaa cggtggcgat cctgcaggca ccagctttcc agctaaatat
      841 gaaattgact atgtgcgggt gtatcaataa gccttaaatc atctggcaat gccttgcaag
      901 ccatcggctt gcaaggcagg tgagtctgca gcagggctgc ctagcccgcg cagggttaat
      961 tcgcacaatc agcacacgag cggcgctaaa accccagtta aacccaatct cgttaaaccc
     1021 aaaccccagt taaacccatg taagacggct gaggtttttc gttttgggct atgcagataa
     1081 ggctaagcaa atagtcgcta aatcgcctat agttttagcg cccctcgttt taccgccccg
     1141 atggtctgcc gcagctaggt atcggatcaa ccaactgata aactaccaaa taagtgatag
     1201 cgtgttactc aacttgcggt ttagaggtgt tttatgagtg tagaaacgtc atcccctcgc
     1261 cgccgagtca ctgacaatcg cctccccgcc ctcgaatggc agctcgtctg ccaagccttc
     1321 caaaatttac cggccatgtt aatggtcaat ctctcggtaa gtgtcggcgg tggtatcgtg
     1381 ctttatagcg acggctattc ctatgtgtac cattggctcg cactggttat tgtgatgaca
     1441 gtggtgcggc tggcggtggg gctttatttt aatgcccgtt acaccaacac catggtggta
     1501 gaccaaaaat ttatcacgct atggaaaggt gtctttgcta ttgggctttt cgccgcagcg
     1561 ggtgtttggg ttgctattgc tatagccaca tttacccacc aagaaattcc ccccacctcc
     1621 cactacacac tgcttattat tatgtcggcc atggccagcg gcgccacagg catcaccgca
     1681 ccgctaaaat taattggccg cttttatatc gccataatga taatacccgc cgccattatt
     1741 atgctgttct actccaataa taatttaatt attggagtat taggtttagt gttttgcgcc
     1801 gccatgattg ccagccacgc ctccaatcat cgtctcctgc gcgcaagcct tacactaaaa
     1861 ctggaaaacg aacaattagt aaataattta cagtacctca actccgattt ggagcgccga
     1921 gtaaacctgc gcacccaagc acttaaacgc atcgcccacc acgactccct cacgggattg
     1981 cccaaccggc gcggcttaat tgaatggatg gagaattcac tggatgctca aaagccccag
     2041 gaagcggcga ttttattttt ggacctggat cggtttaaac aaattaacga tgccctcggc
     2101 cacgatgtgg gcgacaaagt attacagacc atcgccgtgc gctttaacga tctttgccct
     2161 gccaactgta tcttgggccg ctggggcggc gatgaatttt tattaattac ccagcagcgt
     2221 ccagatattc gccaaatggt tgaacatctg gccacgcaat taattgatgc cgccacagcg
     2281 ccgctggaaa tgaacaacga aaaactcggc ctgggcctga gcgtgggcat cgcctacttc
     2341 cccaccgatg cgcgggacta taaagacgtg atccaagccg ctgatttaac cgtggcggaa
     2401 gttaaacgca atggccgcgg ccaaacgctg gtgtataacg acacctatgc cgaaacccag
     2461 cgccgccgtt ttgatttaag ccgcacattg gcggagtcta ttgaacacaa taaattacac
     2521 ttggtttatc agcctattat cgacagccat agcggccagg tggtcgccct tgaagtactc
     2581 tgccgctggc gccaccctgt actgggcgat attaatccgg atgaatttat tcgtttagcc
     2641 gaagacaccg accgcataat tgcccttggc gactgggctc tgcgcaccgc ctgccaaaca
     2701 gcacaaacct ggggcgacaa tgctcgcact attaaaattg ctattaatgt ttcgattaaa
     2761 caattgctca gcaatgacta tgcagggcaa gtctgcgcca ccctcaagcg cctagagttt
     2821 ccgtttgaac gcctggtgtt agaagtaacc gaaaccttat ttggcgaaga atatttagac
     2881 caatccctcg gcactgtcac gcaattgcgc aatttaggca ttgaagtaca tattgacgat
     2941 ttcggcacgg gatattcgtc cctctcccgc ctgcatcaat tccctgtggc agcgattaaa
     3001 attgaccgct cttttataat gcagctggat gctcaaagca tggtaattat tgaaagcgct
     3061 gttatgattg ccaaacgcat gcaattaaaa gtaattgccg aaggcgttga atccgccgcg
     3121 caagccaaca cattaataaa actcggtgtg gattatttac agggctatta ttttggcaaa
     3181 cccagtgaca caccacaatt tgcaccaatt catcttgaga gcctttaaac ctaaggaacc
     3241 tctgaaaaat gtaggcggcg aagcactggc taggcgaatt tgccaaaaaa agcggagttt
     3301 acacgtagta aatgagcatt tttttggcaa attcaacgcc gccagggcga gtaaggtaat
     3361 ttttcagagg ctccctaaaa accgcagctg cagattgccc aaccatggca accaataccc
     3421 tttataaata agagtgaata cctctcacag ggttaataac atggattgaa tgggtatttc
     3481 agccaatatg aatttactaa aacatcatcc aattaatcct tgcacttgtg cacaatcact
     3541 ggcaggatga ggttttttgg gagaaacact atgaagacct caacatggct tcgcactctg
     3601 ttcatctcaa ccctggctta ttcaacagtc gccagtgcag ccacatctgt tattcaactt
     3661 gattttaccg gcgtcggcag ccgcgcagcg gttaagagct tctacagcgg aggggcagat
     3721 agcgacggca actccggcgt caattatggc gttaccttta gcgagaacgc ccttagcctt
     3781 tcagatattg atccagatgc caacttcgca ggcgagccct ccccccacaa tattatgttt
     3841 ttcctctccg ggcccgctta tctcagcata cctgagggtt ttagccaggg gttttcgttt
     3901 tattactcca ccctagattt taccggccaa gtagatatct atgacggcgt ggatgcaacc
     3961 ggcaatgtgc tcggctcatt aacattggat gcgttaggcg aaggccccgt attagaccgg
     4021 ccctacagca attggaaaat agccgcctta aattttaccg gggttgcaaa atcagtgaat
     4081 ttcagcggta cggtcaacga agttggcttt gataacatca ccttagggtc aatagatcct
     4141 tccatccccg ccgtacctga accttctagc actgctcaat tattactggg tgtaggttta
     4201 ttagcgctgc tgcgccgcag gtacagcaca ggcaaaaatc tcagttaaaa taaaaccccg
     4261 cagggcttag cccagcgggg gttattcgca caagctaatc gcggcaatag gcttattccg
     4321 cagccgctcc cacaaaaata ctgtttaccc gccccttatc caattcaaat ataacgccgc
     4381 cataaaccga acctaatacc aggttttgtt ggctgatcga ttcctctgta ttaatttccc
     4441 tcagatattg gctacgcacc tgtgcatctg tactgccgat acctatgcct ttctcagttt
     4501 taagcgtgct tggcgcctca actttcatat cgaatagctt aagtggtgac tttttatcct
     4561 cagcactaaa ccctaaggta aggccgcaac ggggagccct ccaggtttga tgccagccac
     4621 catcggcctc gctggtgaca agcttgccct tggtaaacac acaaccaggc aagcccacca
     4681 cttcagaggc aggcatgcct atgtgtaatt taccaattct aaaagtcgtc aatttaattt
     4741 ctggttgggc ggcctctccg ctccaaactg gggtagcaat gaggcagctg gccaaggcaa
     4801 atagagttaa gtgcaatcgc ataaatagtc tccttaggta atccgtggtt gtttgaaaat
     4861 taaattaatt ttgcgcgaat aagctcgtgt ttaatatagg catataaaat cggcgccatc
     4921 accacacccg gtaaaccaaa taccgattcc atggcaatcg cagcaatcag cagctcccag
     4981 gcattggatt taatctccgt gccgataatt ttcgcattga gaaaatactc caacttatgc
     5041 agcaccacca aaaacaac
//
LOCUS       DAOQGS010000294         5045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8633_length_5045_cov_3.787375, whole genome shotgun sequence.
ACCESSION   DAOQGS010000294 DAOQGS010000000
VERSION     DAOQGS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_5045_cov_3.787375"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PKZ52_11375"
     CDS             complement(<1..1175)
                     /locus_tag="PKZ52_11375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNG60417.1"
                     /translation="MRAPKHPLLHNLRETFWQSLPKQALNESLKRRTLLKGGLASLAL
                     AGCASNTSAKRKPQIAILGAGLAGLMAGYTLKKRGISAHLYDAAERTGGRVFSQENLV
                     ADGLVTELGGEFIDSNHKTLLALAKEFKLGLLDTATDTKVDKYDFYIDNNHYTVEDLL
                     KALQPHSKAILADIDELPDEITYKNIGSARKFDQISIIEYLTQKGISGWLLALFDSAF
                     TTEYGLDAAEQSAINFLFLFDPSLKAKEFEMFGESDERYKIAGGNQQLVDKLAEQLPN
                     IHLSYQVEAISTAGPGYRVLFRNGEDVYFDYVICTLPFTKLREVKLNLEGISPTKRLA
                     INALGYGTNAKFFLGFSKRLWRELGFSGQVFSDTSVQLAWDNSQLQSGQAGGLTVFTG
                     "
     gene            1402..2775
                     /locus_tag="PKZ52_11380"
     CDS             1402..2775
                     /locus_tag="PKZ52_11380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNG60418.1"
                     /translation="MTSISDAAKTACASFWQAARDFGLWRFYAYLGCYLVVFLAALLR
                     IEYIIQYNPIHEIWSDPARHWEQGIDTLRFDPMSMTDPIMYQLYIGMLGKLTLKTPGL
                     VAFYTSILAISGPWLWYKFLRELLPSKLQALIGWALISIIPSWISIYGYFMQETLLIP
                     LLGASLYATWRARRKQDVASFAWMIIFWSIAGLTRGVAIPMAAVAASWVWLEQGQKMR
                     KAIVSLLILGLILGPLTYRAYKIVHVFAPHGMAYMNQIYARSGKKWIEMTYDREGAVW
                     GYGFGSPATGSKPFAPFSDWMTQREGPVTAFVDLDEGKRDWDALFEREALSWDKYSWI
                     LKENIIFLMQSESWPDSNTERLLGAINYVTRWIWPPLLLIGIIWTALIARRMKGVWLF
                     PAMIAAWFFVQALLPIAVNEGRYRKPFEGLIIAHLILLASRTGPIALGRRKQESPVTS
                     TSAQVSV"
     gene            2776..3762
                     /locus_tag="PKZ52_11385"
     CDS             2776..3762
                     /locus_tag="PKZ52_11385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNG60419.1"
                     /translation="MSEQLLSILVPMYNESAVIDRLFEALERTLAQVDMQYEIICVDD
                     GSKDTTRQQVIFHTQRDSRIKAVFFSRNFGKEAAMTAALDYASGDAVIPIDADLQDPP
                     ELIIDMVAKWREGFDVVYAKRVSRDTDTAMKRNSASGFYKLFNAVSEFKIPENVGDYR
                     LMSRRVVDAIKLLPEKDRFMKGLFCWPGFSNTTVEFERQARVDGETKFNYWKLWNFAL
                     AGITSFSSLPIRLGGYLGLCVSAVAFLYGSFIFLRTLITGVDVPGYASLMIVVLFIGG
                     VQLFFMGLMGEYIGRIYKEVKNRPIYLVEQQIGFQYESPRQPLSAPSQDLMR"
     gene            complement(4233..4430)
                     /locus_tag="PKZ52_11390"
     CDS             complement(4233..4430)
                     /locus_tag="PKZ52_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNG60420.1"
                     /translation="MTLTTLSIPCPTCKKNVLMTADFPQRPFCSERCRLIDLGAWADE
                     SHRIAGEAVELDIWTEEVGPK"
     gene            complement(4390..5025)
                     /gene="coaE"
                     /locus_tag="PKZ52_11395"
     CDS             complement(4390..5025)
                     /gene="coaE"
                     /locus_tag="PKZ52_11395"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207943.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNG60421.1"
                     /translation="MAWILGLTGGLAVGKTRVANSFAELGAQLIDADVIARDLVAPGT
                     PALQAIIERFGAGIVQAGALDRPQLRRVFFAEPAAKTWLEQLLHPLIRHAITQQIQLP
                     HSPYAILVAPLLFENGLNSLTQRTLVIDATEEQQLSRGGQRDGSDEQTLKSIIAAQMP
                     RHQRLALADDRLDNSGPWPDTQRAIAVLHHQYQQLAKAYDAHNPVHPLPHL"
BASE COUNT         1113 a         1325 c         1329 g         1278 t
ORIGIN      
        1 ccggtaaaaa ctgtcaggcc gcccgcctgg ccggattgca actggctatt gtcccatgcc
       61 agctgcacgc tggtgtcgga aaacacttgg ccgctaaagc ccagctcccg ccacaagcgt
      121 ttgctaaagc ccaaaaagaa cttggcattg gtgccataac cgagggcgtt aatggccaag
      181 cgcttggtgg ggctgatgcc ctccaggttg agttttactt cccgcaactt agtgaagggc
      241 agggtgcaaa tgacgtaatc aaaatacaca tcctcgccgt tgcggaataa caccctataa
      301 ccagggcctg cggtgctgat ggcttccacc tggtaactta ggtgaatatt gggtagttgc
      361 tcggccagtt tatccaccaa ttgttggttg ccaccggcaa ttttgtagcg ctcatcgctc
      421 tcgccaaaca tttcaaattc tttggctttt aagctggggt cgaataaaaa caaaaaatta
      481 atcgccgact gttctgcggc gtccagccca tattcggtgg tgaaggcgct atcaaacaag
      541 gctaataacc agccggaaat acccttctgg gtgaggtatt caataatgct gatttggtcg
      601 aatttgcggg cgctgccgat atttttatag gtaatttcat cgggcagctc atcaatatct
      661 gccaagatgg ctttgctgtg gggctgcagc gctttgagta aatcctctac cgtgtagtgg
      721 ttgttatcga tataaaaatc gtacttgtcc accttggtgt cggtggcggt atccagtagc
      781 cccagtttga attctttcgc cagcgccagc agggttttgt ggttgctgtc gataaactcc
      841 ccgcccagct cagttaccag gccatcggcc actaaattct cttggctgaa tacacggccc
      901 ccggtgcgct cggctgcatc gtatagatgt gcgctgatgc cgcgtttttt taacgtgtaa
      961 ccggccatta accccgctaa acccgctcct agtatggcaa tttgcggttt gcgtttagcg
     1021 ctggtgttgc tggcacaacc ggccagggcc agacttgcca ggccgccttt aagtagcgtg
     1081 cggcgtttga gggattcgtt taaggcctgc ttaggcaggc tttgccaaaa ggtttctcgc
     1141 aggttgtgta acagagggtg tttgggtgcg cgcatagcag gggctcactt aaaataaata
     1201 accggcaaag tacctgagtt taggctgctg gtacagtcca ccggcgcgct gcacacaaag
     1261 gccaatcaaa ggctgccggt gtgggcggga ttagcttcta taattgccag cagcctgccg
     1321 caagctgtgc ggcagttagc cattaggttt aggccgcttc atgtatcatg cggccttttg
     1381 ttcgagcttg gggagatacg tgtgacatct atatcggatg cggctaaaac cgcctgcgca
     1441 agtttttggc aagccgccag agactttggg ttgtggcgtt tttatgccta tctcggctgc
     1501 tacctggtgg tgtttctggc ggcccttttg cgcatcgaat acatcattca gtacaacccg
     1561 atccacgaga tttggagtga cccggcccgc cattgggagc agggcatcga taccctgcgc
     1621 tttgatccca tgtccatgac cgaccccatc atgtaccagc tgtacattgg tatgcttggc
     1681 aaactcacgc taaaaacccc cggtctggtg gccttttaca cctctattct cgccattagc
     1741 ggcccctggc tgtggtacaa attcctgcgc gagttattac cctccaaatt acaggcgctt
     1801 atcggctggg cgctgatttc cataattccc tcctggatta gcatctatgg ctacttcatg
     1861 caggaaaccc tcctgattcc attgctaggt gcgtccctct acgccacctg gcgggcgcgc
     1921 cgcaagcagg atgtggcttc atttgcctgg atgatcattt tttggagtat cgcaggcctc
     1981 acccgcggtg tggcgattcc catggccgcc gttgcagctt cttgggtgtg gttagagcag
     2041 ggccaaaaga tgcgcaaggc catagtctcc ctgctgattc tgggtttgat cctcgggcca
     2101 ttaacctacc gggcctataa aattgtccac gtctttgccc ctcatggcat ggcctatatg
     2161 aaccaaattt atgcccgctc cggtaaaaaa tggattgaaa tgacctacga ccgcgaaggc
     2221 gcggtatggg gttatggctt tggctccccc gccaccggca gcaaaccctt tgcacccttt
     2281 agcgattgga tgactcagcg tgaaggcccg gttaccgcat ttgttgacct ggacgaaggt
     2341 aagcgggact gggatgcctt attcgagcga gaggccctca gttgggataa gtactcatgg
     2401 atactgaagg aaaatattat tttcctcatg cagagcgaat cctggccgga ttccaatacc
     2461 gagcgcctat tgggggccat taactatgtc acccgctgga tttggccgcc cttgttgtta
     2521 atcggtatta tctggacggc gttaattgcc cgccgcatga aaggcgtctg gttattccct
     2581 gccatgatag cggcctggtt ttttgtgcag gccctattgc cgattgcggt caatgaaggc
     2641 cgctaccgca aaccttttga aggtctaatc attgcgcatt taatcctact tgccagccgc
     2701 accggcccca tcgccctggg ccgccgcaag caggaatccc ccgttacctc cacatctgca
     2761 caggtgagtg tttaaatgtc cgagcaattg ctgtctattt tagtccccat gtacaacgaa
     2821 tctgcggtca tcgaccgctt gtttgaagcc ctggagcgca ccctggctca ggtggatatg
     2881 cagtacgaaa ttatctgtgt ggatgacggc tcaaaagata ctacccgcca gcaggtgatc
     2941 tttcataccc agcgcgacag ccgtataaaa gcggtatttt tctcgcgcaa tttcggcaaa
     3001 gaagccgcca tgaccgccgc cctcgactac gccagcggcg atgcggtgat ccccatcgac
     3061 gccgacttac aagatccgcc ggagttaatt atcgatatgg tggccaaatg gcgggaaggc
     3121 tttgatgtgg tgtacgccaa gcgcgtctcc cgcgataccg acacggccat gaaacgcaat
     3181 tccgccagtg gtttttacaa actctttaat gcggtgagcg aatttaaaat tccggaaaac
     3241 gtcggcgatt atcgcttgat gagccgccgg gtagtggatg ctattaaatt actgcccgaa
     3301 aaagaccgct ttatgaaagg cctgttttgc tggccgggtt tttccaacac cacggtagaa
     3361 tttgaacgcc aagcgcgggt ggatggtgaa accaaattta attattggaa actgtggaat
     3421 ttcgccctgg caggcattac ctccttcagc tcactaccca ttcgtttagg cggttatctc
     3481 ggcctatgtg tatcggcggt agcatttttg tacggctcct ttattttctt gcgcacctta
     3541 attaccggtg tggatgtgcc gggttatgca tctttaatga tcgtggtgtt atttatcggc
     3601 ggtgtgcaat tattttttat ggggctgatg ggggaataca ttggccgcat ttataaagaa
     3661 gtgaaaaacc ggccgattta tttggtggag caacaaattg gctttcagta tgagtcgccg
     3721 cgccaaccat tgagtgcacc gtctcaagat ttgatgcgct aataccaacg cttctgcatt
     3781 tacgtcaccg gttcgctact gcttaatgac ttgaattcag tagctggaat tcagtggccg
     3841 caataaagca cagcgaaatc cgggttattc tcgttcccac gctctgcgtg ggaatgcatg
     3901 ctttgttctc tgcctaatac tcctactcga accccctccc aaaacattcc cacgccgagc
     3961 gtgggaacaa gatcaacgcc taaggaaaca gtcgcaacct aattcccgct gcggagaata
     4021 ataattgaag gatttaatag tcagggcgtt ttaaaattcc cctccatcgg aggggtggcg
     4081 ggcgcagcca gaagggggtg gttgcgctgc ccattccctc cataagccac tggggtgaat
     4141 acacttacca aataagtagg atttttgttt gcgcttaagc gatatacacc aagggtatcg
     4201 caaggatggt tacagcttaa agcctggcca atttatttcg gccccacttc ttccgtccaa
     4261 atatccaact ccaccgcctc acccgcaatc cggtggctct catccgccca ggcccccaaa
     4321 tcaattaacc ggcagcgctc gctgcagaac ggccgctggg ggaagtccgc cgtcattaac
     4381 acgttttttt tacaggtggg gcaggggatg gacagggttg tgagcgtcat aggccttggc
     4441 taattgttga tactgatgat gtaaaacggc tatggccctt tgggtatctg gccaagggcc
     4501 ggaattatcc aggcggtcat cggctagcgc aaggcgctga tggcggggca tttgcgcggc
     4561 gatgatggat ttgagcgtct gctcatcact gccgtcgcgc tggccgccgc ggctaagctg
     4621 ttgctcctca gttgcgtcta taaccagggt acgctgggtc agggaattca ggccattctc
     4681 aaataaaagc ggcgcaacca aaattgcata gggggaatgg ggcaattgaa tttgttgagt
     4741 gatggcgtgg cgaattaacg ggtgtaataa ctgttcaagc caggttttag cggcaggttc
     4801 ggcaaaaaaa acgcggcgca actgcgggcg atcgagcgct cccgcctgca caattcccgc
     4861 cccaaatcgc tcgataatag cctgcaatgc aggggtgcca ggcgccacta agtcgcgggc
     4921 tattacatcg gcatcaatta actgggcgcc taactcggca aaggaattgg ccacccgagt
     4981 cttgcccact gcaaggccgc cggtgagccc tagtatccaa gccaccttca gtaacctcta
     5041 gccat
//
LOCUS       DAOQGS010000295         5017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8706_length_5017_cov_4.955260, whole genome shotgun sequence.
ACCESSION   DAOQGS010000295 DAOQGS010000000
VERSION     DAOQGS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_5017_cov_4.955260"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKZ52_11400"
     CDS             <1..660
                     /locus_tag="PKZ52_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNG60422.1"
                     /translation="GVNHPLGKNMIGAFYQPKGVVIDTQVLATLPDREFSAGMAEVIK
                     YGFIYDADFLVWLEANVEGLMRRDWSLVEAAIVRSCEIKSAVVEADEREQDLRAILNF
                     GHTYGHAIEAHLGYGQWLHGEAVGAGMVMASELSCTIGWISDADHQRVVNLIARCNLP
                     IAPPQGMYSEAFMRYMALDKKVAAGKLKLVLLRALGDAVVTGQFPPEALAAQLDGLST
                     P"
     gene            complement(921..1439)
                     /locus_tag="PKZ52_11405"
     CDS             complement(921..1439)
                     /locus_tag="PKZ52_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60423.1"
                     /translation="MRKKNLKFTASIAPSRLYLLSVLALHTLASVLSLCIDFSVAQHL
                     FALAAIAASLCYQLGVYAQNACWVHVSFEADQAICTKSKINYFPIGDDALALSNFIGD
                     TEEYRAELLPKAWVLPHLVILYVQFKAGSCKSLIIVKDSIAADDFRRLKIFVLNGPLL
                     KNQRLKQGENSV"
     gene            complement(1420..1665)
                     /locus_tag="PKZ52_11410"
     CDS             complement(1420..1665)
                     /locus_tag="PKZ52_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNG60424.1"
                     /translation="MDYNRLKWASRRGMLELDLILQPFVEQAYPTLSAEWQQIFADLL
                     LCEDQDLFAWLLNRQDPEDEDLAKMVRMIRDYAQKKP"
     gene            2276..3196
                     /locus_tag="PKZ52_11415"
     CDS             2276..3196
                     /locus_tag="PKZ52_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60425.1"
                     /translation="MRDINGAENVHSTKYYPLPDYHYIEISGPEAVKFIQSQVTCDMR
                     RLEQQEWLLGAQCNPKGRMVLTFVAFQKQANVYLRVHSSVQQAFEGLKKYAVFTKVKF
                     SLLEMPAYALVGDHILSQVGAALNLLEPLTAGKFQVFEDAVLVARANDWLEVYGTCEA
                     LTQWIEQKQSVIKAENFDAELIKRGIAEVRGPTVEEFIPQMFNLDQVDAISFKKGCYT
                     GQEIVARMQYLGKLKKHLYRGKISAVHLPVGAALHSAEHTEAQGVVVLSSGEEFLAVV
                     NDQAMEHALASPGYEHSKIQWLPLPYAITM"
     gene            3234..4070
                     /locus_tag="PKZ52_11420"
     CDS             3234..4070
                     /locus_tag="PKZ52_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG60426.1"
                     /translation="MSNLVEKISAIILKSIDDDSLVLPTMPEMALRVREVADDPNASI
                     KQLAGIIGQDAALAARIIKVANSAIYRGAKVIEDLNMALSRLGMSTTCALATGLAMEQ
                     MFQATSDLVDRRLRLVWNQSAEIAGICTVLCKFKTKLKPDEAALAGLVHQIGVLPILS
                     YAEDHPALLRDSFTLESVIAQAHPHIGARILTVWGFPAAIQQVPLQYLDFTRVAPKAD
                     YADLVTVGFLQSLDANNIHYQDLDYSTVTAFDRLGLDWDIDNAEAEDLSEAMASATNM
                     LK"
     gene            4329..>5017
                     /gene="mpl"
                     /locus_tag="PKZ52_11425"
     CDS             4329..>5017
                     /gene="mpl"
                     /locus_tag="PKZ52_11425"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNG60427.1"
                     /translation="MGHIHILGICGTFMGSLAVLAKQLGFRVTGSDQQVYPPMSTQLA
                     AAGIELIEGYDPAQLDLNPDVVVVGNVISRGNPVMEAVLNRMIPYTSGPAWLAEHVLN
                     NRWVLAVAGTHGKTTTASLLTWILEYSDYSPGFLIGGVPLNFPASASLGGSSFFVIEA
                     DEYDCAFFDKRSKFLHYHPRTTILNNLEYDHADIFPDLAAIQRQFHHLVRIVPEEGLI
                     IYPAQDKALQQV"
BASE COUNT         1254 a         1131 c         1291 g         1341 t
ORIGIN      
        1 ggcgttaacc atcctttagg caaaaatatg attggcgcct tttatcagcc aaagggtgtg
       61 gtaattgata cccaggtact agcgactttg cctgatcgag aattctccgc aggtatggcg
      121 gaagtgatta aatatggatt tatatacgat gccgattttc tggtttggct agaagctaat
      181 gtagaaggac taatgaggcg tgattggagc ttggtggaag ctgcaattgt gcgttcctgt
      241 gaaattaagt cggcagttgt tgaggctgac gagcgggagc aggatctgcg cgctatttta
      301 aattttggcc acacctatgg acatgctatt gaggcgcatt tgggctatgg ccagtggtta
      361 cacggcgaag cggtgggtgc tggcatggtc atggccagcg aattatcgtg cactatagga
      421 tggattagcg atgcggatca ccagcgcgtc gtcaatttaa ttgcgcggtg taatttgccc
      481 atcgcgccac cacagggcat gtattctgaa gccttcatgc gttatatggc actggataaa
      541 aaagttgctg ctgggaaact aaaattagtt ttattgcgcg ccctcggtga tgccgtggta
      601 acggggcaat ttcctcctga agccctggct gctcagttgg atggcctcag cactccataa
      661 ttgcctacct gatttaccgc gcgtgaagtg ggcgaaataa cgaattttta taggtggtga
      721 ttgtttttat taacccggcg ccatttagtg ccgggttaat aatggcggca tggatgccgc
      781 cccggcggtg caaaccgccg tgagctgccc taaaacaagg aaatccacgc attttcgcca
      841 gttttagggc ggcgagaagg ctaaatagat caggacgatc tatttagccg ccgggttaat
      901 attgggccga ggtttttagg ttatacgctg ttttcgcctt gttttaagcg ttggttttta
      961 agcagaggcc catttaatac aaaaattttt aacctgcgaa aatcatctgc cgcgattgag
     1021 tctttaacaa taatcaggct tttgcagctg cctgctttaa attgtacgta gaggatgact
     1081 aagtggggta atacccacgc cttgggcaag agctcagcac ggtactcttc tgtgtcacca
     1141 ataaagttgg acagagctaa cgcgtcatcg ccgattggga aataatttat tttgcttttt
     1201 gtacaaatgg cctgatcagc ctcaaaactt acatgcaccc aacaggcatt ctgcgcgtag
     1261 acaccaagct gataacacag gctagctgcg atggcggcca gtgcaaatag gtgttgggca
     1321 accgaaaaat caatacatag gcttaggaca ctcgccagcg tatgcaaggc caatacagag
     1381 agcagatata accgtgatgg ggcgatgctg gcggtaaatt taaggttttt tttgcgcata
     1441 atcgcggatc atcctgacca tttttgccaa atcttcatct tccggatctt gtcggttcag
     1501 gagccatgcg aataaatcct gatcttcaca gagtaacagg tcggcaaaaa tctgttgcca
     1561 ctctgcgctg agtgttggat aggcctgttc aacgaagggc tgtagaatca agtccagttc
     1621 gagcatccca cgacgggagg cccatttgag gcggttgtaa tccatggcgt gctcacttat
     1681 gaaaaagtgg gacagtatac tgtctgtttt atacggttgg cagattgcac agggtgtgtg
     1741 tggcacgaaa aagggctcag ggtacagcgc aatccgtgga ccatagattt ccacccggcg
     1801 atacaatggt aaactcctca aaacgagccg cgtgaaagac gctgcgaggg cgtgaaagtt
     1861 aaaattgctt gcctggcatt ggattcccgc agtttcgccc tgcgcgctct gccgacagct
     1921 gtttggcaaa caagccttaa cagcctgttg aaatactccc ttgctagctc tggcggcgtt
     1981 aaattttggc aaaaaattct catttactgc gtgtaaactc ggcaattgct cctgcattgc
     2041 tctacctcct gcatccatgc agtcgtccgg ttttttgcca aaatttgcct taccagagcg
     2101 tcgccgccca agtatttcaa caggctgtta attagagttt cccctatgca gatgcccaat
     2161 agatgaaaaa agatgggcct agcttttggg gaatgaacac ccaagttgac caaaaaatta
     2221 agactaaacg cagaaaaata tctgctgcac acagtgcaaa aattgaggat ttattatgcg
     2281 agatataaac ggcgcagaaa atgtacattc gaccaaatat tacccattgc cggattatca
     2341 ctatatcgaa atatccggcc ctgaagcggt gaaatttatt cagtcgcaag ttacctgtga
     2401 tatgcggcgg ctggagcagc aggagtggct gttgggcgcc cagtgtaatc ctaaggggcg
     2461 aatggtttta acctttgtgg catttcaaaa acaggccaat gtgtatttgc gcgtgcattc
     2521 gagtgtgcag caggcatttg aggggctaaa aaaatacgcc gtatttacta aggttaaatt
     2581 ttcgctgctt gaaatgcccg cttatgcatt agttggcgat catatcctca gtcaagtggg
     2641 ggctgcttta aatttactcg agcctctaac cgccggtaaa ttccaagtgt ttgaggatgc
     2701 tgtattggtg gccagagcga atgactggtt ggaagtgtac gggacttgtg aagccttaac
     2761 ccaatggata gagcaaaagc aaagtgtaat aaaggctgaa aattttgatg ctgagctaat
     2821 taagcgcggt atagctgaag tgcgcgggcc aacggtagaa gaatttatac cgcaaatgtt
     2881 taatctagat caggtggatg ccatcagttt taaaaaaggc tgttataccg ggcaggaaat
     2941 tgtggcgcgt atgcagtact tagggaaatt aaaaaaacat ttatatcgcg gcaaaatctc
     3001 tgctgtacat ttgccggtgg gggcagcact gcacagtgct gaacacaccg aggcgcaggg
     3061 tgtggtggtg ctaagcagcg gtgaggagtt tttggcggtg gtgaatgacc aggccatgga
     3121 gcatgcgctt gccagcccag gctatgaaca cagcaaaata cagtggctgc cgctacccta
     3181 tgctataact atgtgattag gggccggaag gcccccgcgg cttgggagtg cttatgtcta
     3241 atctggtaga aaaaatctcg gcaatcattt taaaatctat tgatgacgac agcctggtgc
     3301 tgccgaccat gcctgaaatg gccttgcggg tgcgggaggt tgccgatgat cccaatgcca
     3361 gcatcaaaca attggcgggg attatcggcc aggatgctgc gctcgccgcc cgtatcatca
     3421 aagtggccaa cagcgccatt taccgcggcg ctaaggttat tgaagactta aacatggcgc
     3481 tctctcgcct ggggatgagc accacctgcg ccctggctac cgggcttgcc atggagcaga
     3541 tgttccaggc tactagcgac ctggtcgatc gccgtttgcg cctggtgtgg aatcaatccg
     3601 ctgagattgc cggtatttgc acggttctct gtaaatttaa aactaaactc aaacccgatg
     3661 aagcggcctt agccgggtta gtgcatcaaa ttggtgtact gccgattctt tcctacgctg
     3721 aagatcaccc cgctttattg cgtgatagtt ttactctgga atcggtaatc gctcaggcgc
     3781 atccgcatat aggtgcgcgt attttgacgg tatggggttt tccggctgct atccaacagg
     3841 tgccattgca atatttagat tttactcgtg tcgcacctaa ggccgattat gccgatttgg
     3901 ttaccgtggg ttttttacag agcttggatg ccaataatat ccattaccaa gatctggatt
     3961 attccacggt tacggctttt gaccgcttag ggttggattg ggatattgat aacgctgaag
     4021 ctgaggattt atccgaagcc atggccagcg cgaccaatat gctgaaataa cagccctttt
     4081 attggagtta cgcccctcat cgcgctccac catctaatac agggagcgcc gtcacttttc
     4141 tcccttgtta tttagccgcc aaggcgtatt ggctaatacg tctgcacttt aattgagtat
     4201 ttttgcgcgc ccaaccttaa gcctttcaac ctttaggtcg taattaaaat aggccttttg
     4261 gcccaccgct aagctgtcaa cccctgtctt attccagtat cctaacggct ttttaggcgt
     4321 gaaagcttat ggggcatatt catatactcg gtatttgtgg cacttttatg ggcagtttgg
     4381 cggtgctggc caaacaattg ggttttcgcg taaccggcag cgatcagcag gtgtacccgc
     4441 ccatgagtac acaattagca gcggcgggaa tcgagttaat tgagggctat gacccagccc
     4501 aattggattt aaacccggat gtggtggtgg ttggcaatgt catcagccgg ggcaacccag
     4561 tgatggaagc tgtactcaac cgtatgattc cctacacctc aggcccagcc tggcttgctg
     4621 agcatgtgtt aaacaatcgc tgggtattgg cggtagcggg cacccacggt aaaaccacca
     4681 cagcgagttt attaacctgg attttagaat attccgatta ttcaccgggt tttttaattg
     4741 gcggcgtacc actgaatttt ccagcttcgg caagcctcgg cggctcgagt ttttttgtaa
     4801 ttgaagcgga tgaatacgac tgcgcatttt ttgataaacg ctctaaattt ttgcactacc
     4861 acccgcgcac cacaatttta aataatcttg aatacgacca cgctgatatt ttccctgatt
     4921 tagcggccat tcagcggcaa tttcatcacc tggtgcgcat agttcccgaa gaaggtttaa
     4981 ttatttatcc cgcccaagat aaagccctgc aacaggt
//
LOCUS       DAOQGS010000296         4999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8747_length_4999_cov_3.665858, whole genome shotgun sequence.
ACCESSION   DAOQGS010000296 DAOQGS010000000
VERSION     DAOQGS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8747_length_4999_cov_3.665858"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1194
                     /gene="cysD"
                     /locus_tag="PKZ52_11430"
     CDS             271..1194
                     /gene="cysD"
                     /locus_tag="PKZ52_11430"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNG60428.1"
                     /translation="MTSDPSYTYSMTHLKQLEAESIHIIREVVAEFDKPVMLYSIGKD
                     SAVMLHLALKAFAPGKPPFPLLHVDTTWKFKEMIAFRDNRAKELGMDLIVHINQEGVD
                     MGISPFVHGSAKHTDIMKTQGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDKN
                     HRWDPKNQRPELWNIYNGRVDKGESIRVFPLSNWTELDIWQYIHLEQIPIVPLYFSAP
                     RPVVERDGVLIMRDDERMELLPGETIEEKMVRFRTLGCYPLTGAVESHAATLPEIIQE
                     MLLTTTSERQGRVIDHDSSGSMEKKKQEGYF"
     gene            1285..2706
                     /gene="cysN"
                     /locus_tag="PKZ52_11435"
     CDS             1285..2706
                     /gene="cysN"
                     /locus_tag="PKZ52_11435"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNG60429.1"
                     /translation="MSHQSDLIATDINAYLAQHEQKELLRFLTCGSVDDGKSTLIGRL
                     LHDSKMIYEDQLEAIKSDSLKHGTTGEKIDLALLVDGLQAEREQGITIDVAYRYFSTA
                     KRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARYGVVTQTRRHSFIASLLGIKHV
                     VVAINKMDLTDFSQEVFEKIKADYLAFSKNLNAQDYYFVPMSALDGDNVVNRSERAPW
                     YQGPTLMEVLEAVPIAQDKNLADFRFPVQLVNRPHLDFRGFCGTIASGVVKPGDALKV
                     LPSGKTSRVKQLINFEGSMEYAFAGQAITITLEDEIDISRGDMLVKADDAVTYTNQLT
                     AHLVWMAESPMQPGKEYLFKFATKVTVGSVDKIHHRIDVNELQLQPAERMQLNDIAKV
                     DVGLQQPVVVDNYQHNRGTGAFIVIDRLTNVTVGAGMVEEARAFSATAAPSEFSAFEL
                     ELNSLIRKHFPHWEAKDLSLLKK"
     gene            2753..3199
                     /locus_tag="PKZ52_11440"
     CDS             2753..3199
                     /locus_tag="PKZ52_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNG60430.1"
                     /translation="MYQVRTPCIGVCSTGIGDVVCRGCKRFAHEVIHWNAYTPAQRRL
                     INGRLDSLLTQLIQPRIEIWDAQKLQAQLFAQKIRHNPDLSAYAWFMDLLKAGASQIT
                     DLNAYGCRWVVADSGQCLVELKQQLDEEFYLLSLVHYERYFAQYLP"
     gene            3196..3843
                     /gene="miaE"
                     /locus_tag="PKZ52_11445"
     CDS             3196..3843
                     /gene="miaE"
                     /locus_tag="PKZ52_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HNG60431.1"
                     /translation="MTDLIAPIRAFLLCATPDAWVQDALARPELMLLDHANCELKAAQ
                     TAMGLIWKYGPAGDECPGTGALLNFTLMQKMSRLIREEMRHFEQVIAIMHKRKIAYEQ
                     LSASRYAAGMRKDVRTYEPARLVDVLIVGAIIEARSCERFDKIAPFLDDELKKFYLSL
                     LKSEARHFQDYLALAKAVAPEPIDGRVAHFLAVDRTLVLSGDGEFRFHSGLLDTL"
     gene            complement(3852..4865)
                     /locus_tag="PKZ52_11450"
     CDS             complement(3852..4865)
                     /locus_tag="PKZ52_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG60432.1"
                     /translation="MSEYVMGVASFITKLLGRNSSPGMHNGDFSPRTVIEPPQFSLME
                     SAQLYLNYLFEQNTAQPITVPQKLVLDLVQSNLKRKENRLNAVPRLPSVIPKLIRSLR
                     DPDSAIRDYVLIVKKDVSMTAAVLKLANSAYFNPIDQHIGDIDRAVMLLGVAGLRSVL
                     SAAVMQPIIQRDSPYFSQTGQRLWQHSLHCAIACEQIAPLRGVEPFSMYLLGLVHDVG
                     KITLFSELCKQFKLNGSQEQPGLQAFAPLMRSFSAQLSYLIARDWDLPEEVCEALKEQ
                     IDLAPGSSISEEGVVLYQANIISEAHVTLYPKFPERAAKLLEHLHLPADLFNTLTSVD
                     EEL"
BASE COUNT         1254 a         1230 c         1264 g         1251 t
ORIGIN      
        1 gcaccgcaag tcccggcacc aaggccgcca gtacccagag catcaggtgc tggatgcgcg
       61 cgccgctgtg ggtatggggg gaggattggc ggataaatgt catggtgaca gcctttaaat
      121 gcaggccggc attctaaggt gacagagtgt aaatatctgc cccagggcct aaaaatctgt
      181 gtggcggcca tggctggcct ggctttcttt tggcatctgc tgccccggcg ggtacaatgg
      241 cggccttttt gatcctaagc ctgtgcccct atgaccagtg atccctcata cacctactca
      301 atgacgcacc ttaagcaact tgaggcggaa agtattcata ttattcgcga agtggtggcg
      361 gagtttgata aacccgtcat gctctattcc atcggtaaag attccgccgt gatgttgcat
      421 ttggcattaa aagccttcgc accgggcaag ccgccgtttc ctttattaca cgtcgatacc
      481 acctggaaat ttaaagaaat gatcgccttt cgcgataatc gcgcgaaaga attaggcatg
      541 gatttaattg tgcacatcaa ccaagaaggc gttgatatgg gtatcagccc ttttgtgcac
      601 ggcagcgcta aacacaccga cattatgaaa acccaaggcc ttaaacaagc cttaaataaa
      661 tacggttttg acgctgcttt tggcggcgcc cgccgggacg aagaaaaatc ccgtgcgaaa
      721 gagcgggtgt attccttccg cgataaaaac caccgctggg acccgaaaaa ccagcgccca
      781 gaattgtgga atatttacaa cggccgcgtg gacaaaggcg aatctattcg tgtatttccc
      841 ttatctaact ggacggagtt ggatatttgg caatacattc atctagagca aatacccatt
      901 gtgcccctgt atttttccgc tccgcgcccg gtggtggagc gcgatggggt attaattatg
      961 cgcgatgacg agcgcatgga actattgccc ggcgaaacca tagaagaaaa aatggtgcgt
     1021 tttcgcaccc tcggctgcta cccgctcacc ggcgcggtgg agtcccatgc ggcaaccctg
     1081 ccggaaatta tccaagaaat gttgttaaca accacctctg aacgccaagg gcgggttatc
     1141 gaccacgata gctcaggctc catggaaaag aaaaaacaag aaggttattt ctaacgtact
     1201 ggtgctggtt atcttggcgt aagcagttat cagcacacct aaacctttat tgtttcactg
     1261 tatagttgtt gacccggagt atgcatgtca caccaatctg atttaattgc caccgatatt
     1321 aatgcgtatt tagcccagca cgagcaaaaa gaactcctgc gatttttaac ctgcggttcg
     1381 gtggacgatg gtaaatccac ccttatcggc cgtttgttac acgactcaaa aatgatctac
     1441 gaagatcaat tagaagcgat taaatccgac tctttaaaac acggcactac gggtgaaaaa
     1501 attgatctag ccctgttggt ggatggctta caagctgagc gggagcaggg tattaccatt
     1561 gatgtggcct accgctattt ctccaccgcc aaacgcaaat ttattattgc cgacacccca
     1621 ggccacgagc agtacacccg caatatggcc accggcgctt ccacctgtga tttggcggtg
     1681 attttaattg acgcccgcta cggcgtagtc acccaaaccc gccgccactc gtttattgct
     1741 tctttactgg gtattaaaca cgtggttgta gccattaata aaatggattt aaccgatttc
     1801 tcccaagaag tatttgaaaa aatcaaagcg gattatttgg cgtttagtaa aaacctcaat
     1861 gcgcaggatt attattttgt gcccatgtcg gcgctggatg gcgacaatgt ggtcaatcgc
     1921 agtgagcgcg cgccctggta tcaaggccca accttgatgg aagtgttaga agcggtgcct
     1981 attgcgcagg ataaaaacct ggcggatttt cgcttccctg tgcaattggt taatcgccct
     2041 catctagatt ttcgcggttt ttgcggcact attgcctccg gcgtggtaaa accgggcgat
     2101 gctttaaaag tcttgccgtc gggtaaaacc agccgggtca aacaattaat taattttgaa
     2161 ggcagcatgg aatacgcctt tgccggtcaa gccatcacca tcactttgga ggatgaaatt
     2221 gatattagcc gcggcgatat gttggtaaaa gcggatgatg ccgtgaccta caccaatcaa
     2281 ttaacggccc atttggtatg gatggcagaa tcccctatgc agccgggcaa agaataccta
     2341 tttaaatttg ccaccaaagt taccgttggt agcgtggata aaattcacca ccgcatcgat
     2401 gtgaatgagc tgcaattgca gccggctgag cgtatgcaat taaacgatat tgccaaggtg
     2461 gatgtgggtt tgcagcaacc ggtggtggtg gataattacc agcacaatcg cggcaccggc
     2521 gcttttattg ttatcgaccg cctaaccaac gttaccgtgg gggccggtat ggtggaagaa
     2581 gccagggcat ttagcgccac ggcagcgccc agtgaatttt ctgcatttga gttggaactc
     2641 aatagtttaa ttcgcaaaca cttcccccac tgggaggcaa aagatctctc cctgttaaaa
     2701 aaataaatgc cttttagcgc ctgcgggcgc ttttaatgca gcggagtatt acgtgtatca
     2761 ggttcgcacc ccctgcatag gggtttgttc taccggtatt ggcgatgtgg tgtgccgtgg
     2821 ctgcaaacgc tttgcccatg aggtgatcca ctggaatgcc tataccccgg cccagcgccg
     2881 tttaattaat gggcgtttgg atagtttgtt gacgcaatta atccagccgc gcattgaaat
     2941 ttgggatgcg cagaagttac aagcacagct atttgcgcag aagatacgcc acaaccccga
     3001 tctatccgcc tatgcctggt ttatggattt attaaaagcg ggcgccagtc aaattaccga
     3061 tttaaacgct tacggctgcc gctgggtggt ggctgattct ggccaatgct tggttgaatt
     3121 aaaacaacaa ttggatgaag aattttattt gctctccctc gtgcattacg agcgctattt
     3181 tgcccagtac ctaccatgac cgatttaatt gcccccatac gcgctttttt attgtgcgca
     3241 acccccgacg cctgggtgca agacgcactg gcgcgcccag aactgatgct gctggatcac
     3301 gccaattgtg aattaaaagc ggcgcaaacc gccatggggt tgatttggaa atacggccca
     3361 gccggggacg agtgccccgg caccggcgcg ctgcttaatt tcaccctgat gcaaaaaatg
     3421 tcccgcttaa ttcgcgagga aatgcggcat tttgagcagg taattgccat tatgcataaa
     3481 cgcaagatcg cctatgagca gttgagtgct tcgcgttatg cggcgggtat gcgcaaagat
     3541 gtgcgcacct atgagcctgc gcgcctggtg gatgtattaa ttgtgggggc gattattgag
     3601 gcgcgctcct gcgagcgctt tgacaagatt gcaccgtttt tagacgatga attaaaaaag
     3661 ttttatttat ccctgctcaa atccgaagcg cgtcactttc aggattacct cgccctagct
     3721 aaagcggtag cgcccgagcc cattgatggg cgagtggcgc attttttagc ggtagaccgc
     3781 acgctggtgc tctctggcga cggtgaattt cgctttcatt ccggattgtt agatacgctc
     3841 tgagcggagt tttataactc ctcatccacc gaggtgaggg tattaaataa atccgctggc
     3901 aagtgtaaat gctccaaaag cttcgccgcc cgctcgggga atttggggta cagggtaacg
     3961 tgggcttcgg aaataatatt ggcttgatac agcaccacac cctcttcgct gatcgagctg
     4021 ccgggggcta ggtcaatttg ttcctttaat gcctcacaca cctcttcagg taaatcccag
     4081 tcgcgggcaa ttaaataact caactgcgcc gaaaagctgc gcattaaggg cgcaaaggcc
     4141 tgcagccccg gctgctcctg gctgccgttg agtttaaatt gtttgcacag ttcgctaaac
     4201 agggtgattt tacccacatc gtgtaccagc cctaataaat acatactaaa aggctcgacg
     4261 ccgcgcaggg gggcaatttg ttcgcaggcg atagcgcaat gcaatgagtg ctgccacagc
     4321 ctttgcccgg tttgggaaaa atagggcgaa tcgcgctgaa taatcggctg catcacagct
     4381 gcggataaaa ctgagcgcag gcccgccaca ccgagcaaca tcacggcgcg gtctatatcg
     4441 ccgatgtgct ggtcgatagg gttaaaataa gcgctgttgg caagttttag caccgccgcg
     4501 gtcatggaga catctttttt cacaatcagc acataatcgc gaatggcgct gtccgggtct
     4561 cttaggctgc ggattaattt ggggataaca ctcggcaggc gcggtacggc attcaagcga
     4621 ttttccttgc gctttaaatt cgactgcact aaatccaaaa ctaatttttg cggtacggta
     4681 atcggctgcg cagtattttg ttcaaataaa taattaagat acagctgcgc tgattccatc
     4741 aggctaaatt gtgggggttc aattaccgtg cgtggtgaaa aatcaccgtt atgcataccc
     4801 ggtgaggaat tacggcctaa aagtttagtt ataaaagacg caacacccat aacgtactcg
     4861 ctcacaatta ccgcaggact tcttcagtat aggccaagtt ccacaggtgg gttgcactgt
     4921 gtcatcccat ctgaaatgag ccacgggccg atccgattga ctcggcggca aaatagatcg
     4981 ccctgatcaa tttagcctg
//
LOCUS       DAOQGS010000297         4984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8788_length_4984_cov_4.188476, whole genome shotgun sequence.
ACCESSION   DAOQGS010000297 DAOQGS010000000
VERSION     DAOQGS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_4984_cov_4.188476"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKZ52_11455"
     CDS             <1..969
                     /locus_tag="PKZ52_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG60433.1"
                     /translation="PTPVFIKSLNPTRLDLPLVLYGRLSPAQSVLLSPSVTETLSAVH
                     VKDGQKVKAGDVLFEMTRQEEQALLSQARAQFKDAQAQFARAETLLKAKLTSLEAFGI
                     KQATFESAQAQLTAVEARLADRLIRAPFDGRLGLKAVNPGDLVRPGDTLAQLEGPEVF
                     HLDIEVPQAQARLIQLGTPFEALSLDDSHTAEGKVLALDNRLQPNTLTLLARGQVAGP
                     GWLAGERVKVRIEHSIEGLTLPEGALVQEGTQSFVFRIEAENKVSRQTVQVKARSGGQ
                     VVVAGVNSGDKIVTQGTAKLTAGAKVAVLGEDTGKPLSELLPRGKP"
     gene            969..4073
                     /locus_tag="PKZ52_11460"
     CDS             969..4073
                     /locus_tag="PKZ52_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG60434.1"
                     /translation="MWVSDTSIKRPVFATVISLILLVAGVASYFRLSLREYPNIDPPV
                     VSISTEYVGAAAATVESRITQIIEDRVAGIEGIRFIESTSEDGRSSVKLEFRLGRDID
                     AATSDVRDRVSRALPSLPPEIRPPDIQKVDANEDVIIWFNLASDGMTVPALTDYAERY
                     LVDRFSSLDGVARVRIGGGQTYAMRIWLDKDKLAAFALTAADVEKALRRENVGLPAGV
                     IESVERQFTLRVNRQFTSAEEFASLVVRADDKGLVRLGDLAQVEKSSVEYRNLFRGNG
                     ANMVGLGIVKQSTANTLDVARVCKQEVARLNKNLPGGMQLLQSYDSSVFIEEAIHEVY
                     RTFAVAAALVILVIYLFLGSWRATLIPAVTVPVSLIATFSLLLMLDFSLNMLTLLALI
                     LSVGLVVDDAIVVLENIHRRMTTLGESALVAAFRGTRQVGFAVLATTFVLVAVFIPIA
                     FVEGDLGRLFTEFAVTMSAAVLFSALVALTLSPVIAARLLTSSHTNNLLIRGVDSGFN
                     SVRGFYQRILQRILHRPWIAGVILVASLAGCIGLYNHLPKEYAPDEDRGTFFVSVDGP
                     EGASYAYMAQYMEEIEKRLMVYIDKGEVDRMLVRAPRNFSASATTFNTGIAIINLKPW
                     GERRDAWAIMADIRKDLADLPGVRIAPIMRQGISSRSGKPIEVVIEGGTYETLAQWRD
                     TLFKAINDNNPGFLALDSDYKQTSPQIKVDINYELAASLGVSVQSIGSTLETLLASKR
                     ASTFVDQGEEYDVLLEGRREQFNDPSDLANVQVRSDTTGALVPLGNLITLSDFADSKT
                     LNRFNRNRAITIEANLETGFTQDKALAYIEQLVKTQLPETAILDYKGRSRAFIDASGA
                     SNWAFILGIVVMFLVLAAQFESYINPLVITLTVPLAIAGGLCGLAIAGMSLNLFSQVG
                     LILLVGLAAKNGILIVEFANQGRDEGLSIEEAIRRAADTRLRPILMTNITALVGALPL
                     IFTPGAGSETRSVLGVTLFSGVLVATVLTLCVVPLVYRLLAPFTHPPLAVTRKLNSAL
                     QDDA"
     gene            4110..>4984
                     /locus_tag="PKZ52_11465"
     CDS             4110..>4984
                     /locus_tag="PKZ52_11465"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNG60435.1"
                     /translation="MVRSDTTLLPLLLTACLHAAPIAAAEEEEFAPEATPQATLAAGR
                     VVLGEPLAQEATLTTYYDSNPQPLWYTQASYPNRQKLLAFIQSSEDEGLLPQDYHLNA
                     LTTGCSGDSMTAELQKNCDWLLSDGFITLANHLANGKVNPTSLTPEWKTQTKLANLGP
                     LLSDISKGRDPIELLAGLRPKSPEYQALRAEIPKLRARLADAPPWEPLALKPAIKIDM
                     EDARLGPIIERLMYWGDLPKTYQSTGRYEAQLQEAIKHFQRRMSLDVDAVIGKSTLVA
                     LNASPEFRLQQLVGN"
BASE COUNT         1051 a         1529 c         1406 g          998 t
ORIGIN      
        1 cccacccctg tatttatcaa atcgctcaac cctacccgcc tggatttgcc gctggtgctc
       61 tatggccgct taagcccggc gcaaagtgtg ctgctctccc ccagcgtgac ggaaaccctc
      121 agtgcggtgc acgtaaaaga cgggcagaag gtgaaagcgg gcgatgtgtt gtttgaaatg
      181 acccgccaag aagaacaagc cctgctgagc caagcccgcg cccaatttaa agacgcccag
      241 gcgcaatttg cccgcgccga aaccctgctc aaagccaagc tcacctcttt agaggccttt
      301 ggcattaaac aggccacctt tgaaagcgcc caggcccaac taaccgccgt tgaagcgcgg
      361 ctggccgatc gcttaattcg cgccccgttt gacgggcggc tgggcttaaa agcagtgaac
      421 ccaggggatt tggtgcgccc cggcgatacg cttgcccagc tggaaggccc ggaagtcttc
      481 cacctggata ttgaggtgcc gcaagcccag gcgcggttaa tacaactggg tacacccttt
      541 gaggctttga gcctggatga cagccatacc gcagagggca aagtgctggc cctggataac
      601 cgcctacaac ccaacaccct gaccctgctg gcgcgagggc aagtagccgg gccaggctgg
      661 ctggccgggg agcgggtaaa agtgcgcatt gaacacagta ttgaagggct caccctgccg
      721 gagggggccc tggtacaaga gggtacccag agttttgtgt tccgcattga agccgagaat
      781 aaggtctccc gccaaacggt gcaggtaaaa gcccgtagcg gcggccaggt ggtggtggca
      841 ggtgttaaca gcggggacaa aattgtcacc cagggcaccg ctaaactcac cgccggggct
      901 aaggttgcgg tgctcggcga ggatactggc aagcccttaa gcgaattatt accacggggt
      961 aaaccctgat gtgggtgtcc gataccagta taaaaaggcc ggtatttgcc acggtcatca
     1021 gtttaatttt gttggtggcg ggggtggcct cttacttccg cttaagcctg cgggaatacc
     1081 ccaacattga cccgccggtg gtgtccatca gcaccgagta tgtgggtgcg gcggcggcca
     1141 cggtggaatc gcgcatcacg caaattattg aagatcgcgt cgcgggcatt gagggcattc
     1201 gttttatcga atccacctcc gaggatggcc gctcctcagt gaagctggag tttcgcttag
     1261 gccgcgatat agatgccgcc accagcgatg tgcgcgaccg ggtcagccgc gccctgccca
     1321 gcctgccgcc ggaaatccgc ccgccggata ttcaaaaggt ggacgccaac gaagacgtca
     1381 tcatttggtt caacctcgcc agcgacggca tgacggtacc cgccctcacc gactatgccg
     1441 agcgttacct ggtggaccgt ttttcctccc tggacggcgt ggcccgagtg cgcattggcg
     1501 gcgggcaaac ctacgccatg cgcatttggc tggataaaga caagctggcc gccttcgccc
     1561 tcactgccgc cgatgtagaa aaagccctgc ggcgggaaaa tgtggggctg cctgccgggg
     1621 tgatcgaatc ggtggagcgc caattcaccc tgcgggtcaa ccgccaattc actagcgccg
     1681 aggagtttgc cagcctggtg gtgcgcgccg atgacaaggg cttggtgcgc ttaggggacc
     1741 tggcccaggt ggaaaaaagc tcggtggagt atcgcaacct gtttcgcggc aatggcgcca
     1801 acatggtggg tttgggcata gtcaaacagt ccaccgccaa caccctggat gtggcccgcg
     1861 tctgtaaaca agaagtcgcc cgcctaaata aaaacctgcc cggcggcatg caattgctgc
     1921 aaagttatga ctcctcggtg tttattgaag aggccatcca cgaggtgtac cgcaccttcg
     1981 ccgtggccgc cgctttagtg atcctggtga tctatctgtt tttgggcagt tggcgggcca
     2041 ccctaatccc tgcggtcacg gtgccagtca gcctcattgc caccttcagc ctgctgttga
     2101 tgctggactt cagcctcaat atgctcaccc tattggcact gattttgagt gttgggctgg
     2161 tggtggacga tgccattgtg gtgctggaga acattcaccg ccgcatgact accctggggg
     2221 aatccgccct ggtggccgcc tttcgcggca ctcgccaggt gggctttgcg gtgttggcca
     2281 ccacctttgt attagttgca gtgtttattc ccatcgcctt tgtggaggga gatttaggcc
     2341 gcctgttcac cgaatttgcc gtcaccatga gtgcggcggt attgttctca gctctagtag
     2401 ccctcaccct ctcaccggtc atcgccgccc ggctgcttac atccagccac accaataacc
     2461 tgctgatccg cggcgtagac agtggcttca actccgtgcg ggggttttac cagcgcattc
     2521 tgcaacgtat tttgcaccgc ccctggattg caggcgtgat cctggtggcg agcctcgccg
     2581 gctgcatcgg tttgtacaac cacctgccca aagagtacgc gccggatgaa gaccggggga
     2641 cgttttttgt gtcggtagac ggccccgagg gcgcctccta cgcctatatg gctcagtaca
     2701 tggaggaaat cgagaagcgc ctgatggtgt atatcgataa aggtgaggta gaccgtatgt
     2761 tagtgcgggc gcctcgcaac tttagcgcca gcgccaccac ctttaatacc ggcattgcca
     2821 tcatcaacct caaaccctgg ggcgagcggc gcgatgcctg ggccatcatg gcggatatac
     2881 gcaaggatct ggcggatcta cccggtgtgc gtatcgcccc catcatgcgc cagggcatca
     2941 gcagccgcag cggcaaaccc attgaggtag tgatcgaagg cggcacctat gaaaccctgg
     3001 cccagtggcg ggacaccctc ttcaaagcca tcaacgacaa caacccgggc tttctcgccc
     3061 tggattccga ctacaaacag accagcccgc aaattaaggt ggacatcaac tacgagctag
     3121 ccgcaagcct gggggtttcg gtgcaaagta tcggctccac cctggaaacc ctcttggcct
     3181 ccaagcgggc ctccacattt gtcgatcagg gcgaggagta cgacgtgctg ctggaagggc
     3241 gccgggagca atttaatgac cccagcgacc tggccaatgt acaggtgcgc tcggatacca
     3301 caggcgcctt agtgcccctg ggcaacctga tcaccttgag cgacttcgcc gactcgaaaa
     3361 ccctcaaccg ctttaaccgc aaccgcgcca tcaccattga ggccaaccta gagaccgggt
     3421 ttacccaaga caaagccctt gcctacatag agcagctggt gaaaacccag ctgccggaaa
     3481 ccgccatcct cgattacaag ggccgctccc gcgccttcat cgacgccagt ggcgccagca
     3541 actgggcctt tatcctgggc atagtggtga tgtttttggt gctggcagca cagtttgaaa
     3601 gctacatcaa ccctttagtg attaccctca ccgtgcccct agccatagct ggcggcctgt
     3661 gcggcctggc cattgcgggt atgagcctga atctgttcag ccaggtaggg ctgattttgc
     3721 tggtggggtt ggcggccaaa aacggcattt taattgtcga gtttgccaac cagggccggg
     3781 atgaaggcct ctccattgag gaagccatcc gacgggcggc agatactcgc ctacgcccca
     3841 tcctcatgac caatatcacc gcattggtag gggctctccc cttaatattt acccccggcg
     3901 cgggcagcga aacccgcagc gtattggggg tgaccttgtt ttcaggcgtc ttggtcgcta
     3961 ctgtgctcac cctttgtgtg gtgcccctgg tgtaccgatt gctggcaccc tttactcacc
     4021 cccccttagc cgttacccgt aaactcaact ccgccctgca ggatgatgct taaatgatgg
     4081 ttcgatgtac tccaaggacg ctatacaaga tggttcgctc cgatacaact ctgctcccac
     4141 tgctgttaac cgcgtgcctg cacgcagccc ccatagccgc cgctgaagaa gaggagttcg
     4201 cccccgaggc gacgcctcaa gccaccctcg ccgcaggccg ggtggtgctc ggtgagccgc
     4261 tggcccaaga ggccacactc acaacctatt acgacagcaa cccccagccg ttgtggtaca
     4321 cccaagcaag ctacccgaac cggcaaaaac tgctggcgtt tattcaaagc agcgaggatg
     4381 aaggcctgct gccgcaggat tatcacctca acgccctcac caccgggtgc agcggcgata
     4441 gcatgacggc agagttgcaa aagaattgcg attggttgct cagcgacggc tttatcaccc
     4501 tggccaatca cctcgccaac ggcaaggtaa accccaccag cctcaccccc gagtggaaaa
     4561 cccaaaccaa gctggccaac ctaggccccc tacttagcga tatcagcaag ggccgcgatc
     4621 ccattgagtt gctggcaggg ctgcgaccta aatcccccga atatcaagcc ctgcgcgcag
     4681 aaatccccaa actgcgcgcg cgcctggccg atgccccgcc ctgggagccc ctcgccctta
     4741 aacccgccat caaaatcgat atggaagacg cccgcttagg gccgatcatc gagcgcttga
     4801 tgtactgggg cgacctgcct aaaacctatc agtccaccgg gcgttacgaa gctcaattgc
     4861 aagaggccat caaacacttt cagcggcgga tgagcctgga tgtggacgcc gtgattggca
     4921 aatccaccct agtggccctc aatgcgagcc ccgaatttcg cctccagcag ctggtgggca
     4981 attt
//
LOCUS       DAOQGS010000298         4969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8829_length_4969_cov_5.350427, whole genome shotgun sequence.
ACCESSION   DAOQGS010000298 DAOQGS010000000
VERSION     DAOQGS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_4969_cov_5.350427"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKZ52_11470"
     CDS             <1..251
                     /locus_tag="PKZ52_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNG60436.1"
                     /translation="QHWMEKQVYLNMGFFLLGTAALGLDAVPIEGIDRDVLNRELHLT
                     HRGLTGVALVAVGYGADTDFNASLPKSRFAAEQIMSFI"
     gene            complement(292..3126)
                     /locus_tag="PKZ52_11475"
     CDS             complement(292..3126)
                     /locus_tag="PKZ52_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HNG60437.1"
                     /translation="MINRQKMSDPIVEPVDRNRLLELRARFLALNSLKLQRVKEGLTL
                     TQQQFLRLLPVLLQVNHPSFPGFVDKSTPVGLVGFNWDKDIIAAARSLSRGFTPQRFL
                     REEAKILSLFLMGSLGTIAQTPRSDFDIWLCYQPKLSATELAKLEQKAQLISQWAQTL
                     GLEVHFFLMNHQAFLQGDLLPMSEESSGELQRILLLDEFYRTSIWLAGRLPVWWLVPA
                     AVERRGEYARYIAVLLRNHFISDQDYIDFGASAHFTAEEFVGAGLWQLHKAIEAPYKS
                     VLKLVLLEAYVNYQAEFSPLSLLHKQKIHAGRDDLNELDPYIALYQFLEDYLLRRSDS
                     VRLEVVRRSFYFKVNRGISKKIGMGAKSWQRQVLERMCRHWGWSSARIAYLDERMRWS
                     LKDVMAENDALVATLMRSFLELNVAARHSGLNNKLCGDDFRLLSHKLTAAFGVKPGKQ
                     LLINPGIKKDLTTQQLWLKKPTPQTWALADSNGGNDLYTANSVQELLLWCSVNSIISA
                     HSKLIWPAKPDAGTDRARTLMHNWVVQNNTPIALAAFKQEPRVEALLVIINWHTAPAD
                     FNPAASGGAFDVLNYGPNQLNLIQRLDCFTRNSWGEWACVSFSGQEALVDYLRWCWRE
                     LTDTCWQWMQCETVASHLSTACSQRVQQLMRALFNCFNRSDMRQRRFLFSFGGKHCCL
                     QWQNDDLLPLTIYSERDLWNFLAVEQEVFCPLVVDTYCLPQQPLAVIGHHVQPGVSQL
                     FYCVAAQTIVLYICDDKGAILRLQLETQEKQQQLVHWIGFLRRALALWQDQHPHNLAD
                     INSLALFEIIPQQQGFAVQRKAVHLEIPAPVLAATIAGGWSDDAQITLSCAGESATWP
                     YLASSGPLNRNLAKTKSADPSGEESAWDHMARWLTPLLPAGQGIIRLDELGWPGATKD
                     WQLVSLVKCKLMVETQLNIARIRQRKREGS"
     gene            complement(3635..>4969)
                     /locus_tag="PKZ52_11480"
     CDS             complement(3635..>4969)
                     /locus_tag="PKZ52_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNG60438.1"
                     /translation="ALNSAVTADGGEIGRAQALQAQAAGIPTSVHMNPILLKPNSDTG
                     AQVIVQGRALSNMEAGNYHHYKKQAKTAVLDSIAQLAASYQAIIVEGAGSPAEINLRR
                     GDIANMGFAEAVDCPVILIADIDRGGVFAHLLGTLMLLSQAEQARVVGFVINRFRGDL
                     SLLQPGIDWLEQVSGRPVLGVIPYLHLLHLEAEDAINQQQTAKNNRPFQVAVPLLPHM
                     SNHTDFDALRVNPAVNLQFVPLDQINCAADLIILPGSKNVRFDLAQLKQFGWQAKLHQ
                     HLRWGGKLLGICGGLQMLGNHIADPLGLEGPAGESEGFGLLDFATELHPHKTLKTVQA
                     RAVHPRLLGCTVQGYEIHAGVSSGPAFNNPLFACEGYFDGCMDDSQAVMATYLHGLFN
                     QPAAQQALLHWAGLVDASASDYAQVQEQSLNRLADTLEAHIDIPLLKTFLYQ"
BASE COUNT         1298 a         1279 c         1174 g         1218 t
ORIGIN      
        1 cccagcattg gatggaaaaa caagtgtatt tgaatatggg ctttttcctg ttgggtactg
       61 cagccctcgg tttggatgcg gtacccattg agggcatcga cagagacgtt ttaaaccgcg
      121 agctacattt aacacacaga ggcttgaccg gcgtggccct tgtggcagtg ggttatggtg
      181 cggataccga ttttaatgcc agcttgccta aatcccgatt tgctgctgag caaatcatga
      241 gttttattta aacctaaaca ccgcagtcaa accctactgc agtttttagg tttaactccc
      301 ctctcgtttg cgctgccgga tgcgggcgat atttaattgc gtctccacca tgagtttaca
      361 tttcactaag gagaccagtt gccagtcttt agtggcgccg ggccaaccta attcatccag
      421 gcgaataatg ccctgcccgg cgggcaacag cggcgtgagc caacgcgcca tatgatccca
      481 ggcggattcc tcgcctgatg gatcggcgga tttggttttg gcaaggttgc gatttaacgg
      541 cccactactg gccaggtatg gccaggttgc gctctccccg gcgcaggaaa gggtaatttg
      601 ggcatcgtcc gaccagccac cggcaatcgt tgccgccaat accggggcgg ggatttcaag
      661 gtgcaccgct ttacgctgta ccgcaaaacc ctgctgctga ggaataattt caaataatgc
      721 caaactatta atatccgcca aattgtgcgg atgctgatcc tgccagaggg ctaaggcgcg
      781 gcgtaaaaat ccgatccaat gcaccagctg ctgttgtttt tcctgagttt ccagctgcaa
      841 acgcaatatt gcgcctttat catcacagat atacaaaaca atagtttgcg ccgccacaca
      901 ataaaataat tggctcacac ccggctgtac atgatggcca atgaccgcca agggctgctg
      961 aggcaggcaa taggtatcaa ccaccaaggg gcaaaatact tcctgttcca ccgctaaaaa
     1021 attccataaa tcccgctcgc tataaatggt gaggggcaat aggtcatcgt tttgccattg
     1081 caggcagcag tgtttaccgc cgaatgaaaa taaaaaacgg cgctggcgca tatcggagcg
     1141 attaaaacag ttgaataaag cgcgcattaa ttgctgcaca cgctggctac aggctgtgct
     1201 taggtggctg gccacagtct cgcactgcat ccactgccag caggtgtctg ttaattcacg
     1261 ccaacaccag cgcaaataat ccaccaaagc ttcctgcccg ctaaaggata cacacgccca
     1321 ctccccccaa ctgttgcggg taaaacaatc caggcgctgg attaaattta attgattggg
     1381 gccgtaattt aatacatcga atgcaccgcc actggccgca ggattaaaat ccgctggcgc
     1441 ggtgtgccaa ttaataatca ccaatagtgc ttcaacacgg ggctcctgtt taaacgccgc
     1501 aagtgcgatg ggggtattat tttgcaccac ccaattgtgc attaatgtac gtgcgcgatc
     1561 tgttccggca tcaggcttgg cgggccaaat cagtttagag tgagccgaga taatcgaatt
     1621 tacactacac cagagcaaca attcctgcac actattggcg gtgtataaat cattgccgcc
     1681 attgctatca gccaaagccc aggtctgcgg cgtcggcttt ttaagccaca actgctgggt
     1741 cgttaaatct ttttttatgc ctggattaat cagcagttgt ttacctggct taacgccaaa
     1801 ggctgcagtt aatttgtggc tgagcaggcg gaaatcgtcg ccgcagagct tattgttgag
     1861 gccgctgtgg cgcgccgcta catttaactc taaaaatgag cgcatgaggg tggcgaccaa
     1921 ggcgtcgttt tccgccataa catcttttag gctccagcgc atacgctcat ctaaataggc
     1981 gatgcgcgct gaactccagc cccagtggcg gcacatgcgc tccaacactt gccgctgcca
     2041 agattttgcg cccataccta tttttttgga aataccgcga ttgactttaa aataaaaact
     2101 gcgccgtacc acctcaagcc gcacagagtc cgagcgccgc agtaaataat cctccaaaaa
     2161 ttgatagagg gcaatataag gatccaactc atttaaatca tcgcggccgg cgtggatttt
     2221 ttgtttgtgc agcaggctca gcggtgaaaa ttccgcttgg taatttacat aggcttccag
     2281 cagcactaat ttcaatacgg atttataggg ggcttcaatg gctttgtgta attgccaaag
     2341 cccggcgcct acaaattctt ctgccgtaaa atgggctgag gcgccaaaat cgatatagtc
     2401 ttgatcgcta ataaaatggt tgcgcagcaa taccgcaata taacgcgcat attcccccct
     2461 gcgctcgaca gctgcaggca ccaaccacca caccggcaga cgcccagcga gccaaatact
     2521 ggtgcggtaa aattcatcca acaataaaat acgctgtaat tcgccgctgg actcctccga
     2581 cataggcaat agatcgccct gcaaaaaggc ctgatgattc atcaaaaaaa agtgaacttc
     2641 caggccaagt gtctgcgccc actggctaat taattgcgct ttttgctcta atttggccag
     2701 ctcggttgca gataatttgg gctgataaca cagccaaata tcaaaatcac tgcggggtgt
     2761 ttgggcgatg gtgccaaggc tgcccattaa aaataaactg agaattttag cttcttcgcg
     2821 cagaaaacgc tgaggcgtaa agccacggct taaactgcgg gcggcggcaa ttatgtcttt
     2881 atcccaatta aagcccacaa ggcccacagg tgtgctttta tccacaaaac ccggaaaaga
     2941 cgggtgattg acctgcaaca aaactggcag caggcgtaga aattgctgtt gggtaagtgt
     3001 gagcccttct tttacgcgct gcagcttaag actatttagc gctaaaaaac gcgcacgcag
     3061 ttccagcaag cgattgcggt ccaccggctc aactataggg tcactcattt tttggcggtt
     3121 aatcataagc tgtgtctaaa acacctcaaa aacgcctaaa caatttgttt tttaatccct
     3181 aaagatgcat ccatgcttgg atgtgaacta aatagttata accaaccact attctttcgc
     3241 tcaacttccc caatagtaga ccctcgacac actatggtgg cggttagata aaaatagtct
     3301 tagccagcca ggtttagata gaaaatgtta aacccagact tagcgcctgg cgctcaaggt
     3361 ttagagttga caatttttgt tggtattaaa gggtgcgctt tgggttttag cccaaaccag
     3421 ggtattggtg gcaaagcgtt acactaagaa ctccagggta actttcggcc ataaacccat
     3481 gggcttaatg tctgtaatgc gcgggagatt tttcaaggcc acgggctgct tattaaaagg
     3541 cggcttttta tatttatatt gcgcgcactg aataaaatcg gcagcgcgct aatataaaac
     3601 ctgtttttta gaatgactct gctgatttta tcgcctattg atacaaaaac gttttaagca
     3661 gcggaatatc tatatgcgct tctagggtat cggcgaggcg attgaggctc tgctcctgca
     3721 cctgggcgta atcgctggct gaggcatcca ctaatcccgc ccaatgtaac agcgcctgct
     3781 gtgcagccgg ttgattaaac aagccgtgca gatatgtagc cataaccgct tggctatcat
     3841 ccatacagcc gtcgaaataa ccctcgcacg caaacaaggg attattaaag gctggacctg
     3901 aactcacacc ggcgtgaatt tcatagccct gcaccgtgca gcctaataaa cgcgggtgaa
     3961 cagcccgggc ctgcactgtt ttaagggttt tatggggatg taattcagtg gcaaagtcca
     4021 agaggccaaa accctcgctc tcccccgcag ggccttctaa ccctaaaggg tcagcgatgt
     4081 ggtttcccag catttgcagg ccgccgcaga tgcccaacag cttaccgccc cagcgtaaat
     4141 gctggtgcag ttttgcctgc caaccaaatt gttttagttg agccagatcg aagcgcacat
     4201 ttttactgcc tggcagaata attaaatccg ctgcgcaatt aatttgatct aaaggcacaa
     4261 actgcagatt aactgcagga ttcacccgca aggcatcaaa atccgtgtga ttactcatat
     4321 gcggcagcaa gggcacagcc acctgaaatg gccggttatt tttagccgtt tgttgctgat
     4381 taatggcatc ttcagcttcc aaatgtaata agtgtaaata aggaattaca cctaacacgg
     4441 ggcggccgct gacctgttcc aaccaatcaa ttcccggctg taataaactt aagtcgccgc
     4501 gaaagcgatt tataacaaac cccaccaccc gcgcctgttc tgcttgggat aacagcatca
     4561 aagtgcccag cagatgagca aacacaccgc cgcggtcaat atcagcaatt aaaattaccg
     4621 ggcagtccac cgcctcggca aagcccatat tggcaatatc gccccgccgc aaattaattt
     4681 ccgcagggct gcccgcgccc tccacaataa tcgcttgata actggccgcc agctgggcga
     4741 tggaatcgag caccgcagtt tttgcctgtt ttttgtagtg gtggtaattg cctgcttcca
     4801 tattggataa tgcgcgccct tgcacaatta cctgagcccc ggtgtcgcta ttgggtttta
     4861 gcaaaatggg attcatatgc acgctggtgg gaattcctgc tgcctgggcc tgcagtgctt
     4921 gggcgcggcc aatttctccg ccatcggcgg taacggcaga atttaacgc
//
LOCUS       DAOQGS010000299         4957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8863_length_4957_cov_4.321093, whole genome shotgun sequence.
ACCESSION   DAOQGS010000299 DAOQGS010000000
VERSION     DAOQGS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8863_length_4957_cov_4.321093"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKZ52_11485"
     CDS             <1..266
                     /locus_tag="PKZ52_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60439.1"
                     /translation="GDLQAYLLVQFLPMLIIPLILLLFPVNHSRPSAYWLLLVCYLLA
                     KVCEHFDTVIYELGLGLSGHTLKHLIAAAGIFLLAVNYRRRTA"
     gene            655..1272
                     /gene="slmA"
                     /locus_tag="PKZ52_11490"
     CDS             655..1272
                     /gene="slmA"
                     /locus_tag="PKZ52_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HNG60440.1"
                     /translation="MTPTPPAKPRRQQILDAFASMLEASPGVRITTAALAKEVGVSEA
                     ALYRHFPSKTKMFEGLIDSIEEAILVPAAAIAAKDELTALNRCEEIFALLLQFCQSSP
                     GVTRILTGDALAGETDRLHLRIVHFYERLESILKQLLREAELAEGLEFSLPVSQTVHL
                     WLALVEGRIAQYVRSQFSQSPLTQWKESWTVMYRGFIRRSEVSLA"
     gene            complement(1241..2029)
                     /locus_tag="PKZ52_11495"
     CDS             complement(1241..2029)
                     /locus_tag="PKZ52_11495"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNG60441.1"
                     /translation="MTETHPILSDSELLRYSRHILLPEVDIVGQEKLAQARVLIIGLG
                     GLGSPVALYLAAAGVGRLLLADHDEVDLSNLQRQVIHQTASLGLNKALSAQQQIAQLN
                     PNCHTDVLPAYLYDAQLNAVLPEVDVVVDCSDNFATRQSINRACVAAGKPLVSGAAIG
                     FSGQIAVFNLGESSPCYSCLYPELPEAQLSCSQSGVIAPLVGVIGSYQALEAVKILIG
                     CGESLAGRLQLFDGLSGQWQTIKLAKDPACPVCGTKPTTPPNGE"
     gene            complement(2026..2865)
                     /gene="prmC"
                     /locus_tag="PKZ52_11500"
     CDS             complement(2026..2865)
                     /gene="prmC"
                     /locus_tag="PKZ52_11500"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNG60442.1"
                     /translation="MSLSIRQALALSTRLEADTARLDIELLLGHCLGKPRSYLRAWPE
                     RLLTEAEQRQFHELLCRRQGGEPMAYILGEREFWSLPLAVSPATLIPRPETELLVETA
                     LALPLPHNTCALDLGTGTGAIALALAQERPQWRLTATDRAPQALALAQANSQRLGLPL
                     ELIESDWFSALAGRRYHLILSNPPYIDACDPHLHVGDVRFEPSSALVAGANGLQDLFT
                     IIEQAPSFLTPGGWLLLEHGWQQAQAVVAALSERGFSQVRWQADLAGQPRMSLGQWPE
                     NLK"
     gene            complement(2881..3333)
                     /locus_tag="PKZ52_11505"
                     /pseudo
     CDS             complement(2881..3333)
                     /locus_tag="PKZ52_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(4374..4957)
                     /locus_tag="PKZ52_11510"
                     /pseudo
     CDS             complement(4374..4957)
                     /locus_tag="PKZ52_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
BASE COUNT          847 a         1321 c         1582 g         1207 t
ORIGIN      
        1 acggggattt acaggcatac ctattggtac agtttcttcc catgctgata attcctctca
       61 tcttgctgct ttttcccgtc aatcattccc gcccatcggc ctattggttg cttctggtct
      121 gttatctgtt ggccaaggtt tgcgagcact ttgatacggt tatttatgag ctagggttgg
      181 ggttgagtgg acacacactc aaacacctca tcgccgcagc ggggatattt ctgctggccg
      241 taaattaccg gcgacgcacc gcttaattga agcaataggt gctgctttta gtcgctagta
      301 ttttctcttc ttgggtgcat ctttaataaa cttttcatca acactttgcc cgccgctaga
      361 ggatgcatgg ccttgagcct gttcgttttt tcggcaggtg acgcatttgt cggctggcgt
      421 caaaatgcgg tggaagcttg tgtgccaaat gtgatttgat ctaggcatag tttctgctaa
      481 ctactcgcaa atgcgtgtgt gactcactct tggatgcggc ttgcccctgg gctgtttggg
      541 ggtagatagc tgcggtgagt gcgcatttgg cagggctgta ccccgtaatt gcggggttct
      601 ttagctgcga acgctgtaat tagcctatta ttcttacaat aagagcgatg taccatgacc
      661 ccgacacccc ccgcaaaacc ccgccgtcaa caaattctcg atgcttttgc cagtatgctc
      721 gaagcgagcc ctggcgttcg catcaccacc gccgctttgg cgaaggaagt tggtgtgtca
      781 gaagctgccc tttatcgtca ctttcccagt aaaactaaaa tgtttgaggg cctgatagat
      841 tccatcgagg aggccatttt agtaccggcg gcggctatag ccgccaaaga tgagctgacg
      901 gcgctcaacc gctgtgaaga aatatttgcc ctgctgctgc agttctgcca gagcagccct
      961 ggagtcaccc gcatacttac cggggatgcg ctagcgggtg aaacggaccg gttgcatctg
     1021 cgcatcgtgc acttttacga gcgcttggag agtattctca aacagttatt gagggaggcg
     1081 gagctggccg aaggcttgga attctccctg ccggtgtcgc aaaccgtgca cctgtggctg
     1141 gcgctggtgg aagggcgcat cgcccagtat gtgcgcagcc agttcagcca gtcgccgctg
     1201 actcaatgga aggagtcttg gacggtgatg taccgaggtt ttattcgccg ttcggaggtg
     1261 tcgttggctt agtgccgcac accgggcagg ctgggtcttt ggcaagcttg atggtttgcc
     1321 attgaccgct gaggccatca aagagttgta acctgcccgc taggctctcg ccacagccga
     1381 tgagtatttt caccgcttca agggcctggt agctgccgat gacacccact aaaggtgcga
     1441 tgaccccgct ttggctgcag ctgagctgag cttcgggcaa ttccgggtag aggcagctgt
     1501 aacaggggga cgactcgccc aggttgaaca ccgctatctg cccgctaaag ccaatggccg
     1561 cgccacttac caggggtttt ccggccgcca cgcaggcgcg gttgatgctt tggcgggtgg
     1621 cgaaattatc gctgcagtcc accaccacat ccacctctgg cagcactgca ttcagctggg
     1681 cgtcgtacag gtaggccggc aaaacatctg tatggcaatt ggggttgagc tgggcaatct
     1741 gctgttgtgc cgatagggct ttgttaaggc ccaggcttgc cgtttgatgg atgacctggc
     1801 gctgcaggtt ggacagatct acctcgtcgt ggtcggcgag caataaccgg cctacacctg
     1861 cagcggccag ataaagggcc accggggagc caaggccgcc gagcccgatg attaacactc
     1921 gtgcctgtgc caacttctct tggccgacta tatctacttc aggtagcaga atatggcggc
     1981 tgtagcgcag taattcgctg tcgctgagga ttgggtgtgt ttctgtcatt taaggttttc
     2041 cggccattgc cccaggctca tgcgcggctg ccccgccaaa tctgcctgcc agcgcacttg
     2101 gctaaagccg cgctcgctca atgcggctac cacagcctgg gcttgttgcc agccgtgctc
     2161 cagcagcagc caaccgccgg gggttagaaa gctgggtgcc tgctcaataa tggtgaatag
     2221 atcctgcagc ccattggcac cggccactag ggcgctgctc ggttcaaagc gcacgtcgcc
     2281 cacgtgtaag tgggggtcgc aggcgtctat gtacggcggg ttgctcagga tcaggtgata
     2341 gcggcggccc gcaagggcgc taaaccaatc cgactcgatc agttcaaggg ggaggccaag
     2401 gcgttggctg ttggcctggg ccagggccag ggcctgtggc gcgcggtctg tggcggtaag
     2461 gcgccactgg gggcgctcct gggcgagggc cagagcaatg gcgccggtgc cggtgccgag
     2521 gtcgagggcg caggtgttgt ggggtagggg aagcgccagt gccgtttcca ccagcagctc
     2581 agtctcgggc cgagggatga gggtggcggg gctcaccgct aggggcaggg accaaaactc
     2641 ccgttcgccg agaatatagg ccatgggttc gcccccctga cggcggcata agagctcgtg
     2701 aaactgccgc tgttcggcct cagtgagcag ccgctcgggc caggcccgca ggtagctgcg
     2761 gggtttgccc aggcagtggc ccagcagcag ctcaatatcc agccgggcgg tatcagcctc
     2821 taggcgggtg cttagggcta gggcttggcg gatgctcagg ctcattggcc gcgagaatgg
     2881 gccgtgggcg atttcttctt cgtcggcttc gcgcacggct tcaatgcgca catcaaagtg
     2941 cagcactttg cccgctaggg catggttgga atccacggtc acttcatcgt cggtgacatc
     3001 cttaacgatc acgtattgaa tgtggttatc gtccgcctgg gcctggaact ccatgccgac
     3061 ttcaatggtg tctatgccgc taaaagctgc gcggggcagg gtttgaatca ggtcggggtc
     3121 gatctcgcca taggcgtctt ccggctgcac aactacctta agactctctc ccgccgtttt
     3181 gccagcgagg gcgcgctcaa ggcccacaat aatgtgcccg aggccctgga tgtagctcat
     3241 gggctctgaa ccggctgagg agtcaatgat gttgccatcc tcgtcagtca gggtgtaatg
     3301 gatggataca acgcagttgt ctgcaatttt cacggatgac tcctcgcgcc cgaagcgcat
     3361 aaaaacggcg cgaagcgcca aaaatcaagc ggtattaccg cttggtgtgg gttgggtggg
     3421 ggcgctgcgg cgccccggtt ggctaaggct gtgtcttata agtggcgggt gtagcggtgt
     3481 ccagcgtttt tacgcgttta cagctcatcc ccggtggtgt ggggtttttt gtacccctta
     3541 taaccgcccc ggttattgct gcttttatag ggtttcggtc cttgggaggg cgcattgccg
     3601 gggcttacgt agctgccgga gccgctggtg taagcggctt tggccggttg aggggaatag
     3661 ggctttttcg ccccttggcc ctgatagcca gcttggggtg acttgccctt ataaccgccg
     3721 ttgtaggcct gttggccgtt gctgtgtttg tggccattgg ccgggcgttt gtggatgggt
     3781 ttgtcgtcca gggccttgag tttatccagg gggatgtcgg cgcggttgcc gaaggactgc
     3841 agcttggcct cagtgcggta atcgccatcg gcggggcgtt gatacacgcc tacataggcc
     3901 ggctgggtag ggttcttttt gccgccatag gcgggtttgc ctgtgcctgt atgcttgggt
     3961 ttatcctgcc aggagcggtt gtccccggcg ccgttgtaag gttttttatc ccctcgctcg
     4021 aaggatttag gcttgccctc gtgggatttg tgggggtgtt ttttaaagcc gcctttgccg
     4081 tggttaggcc gcgctggccg ctcggcacct tcgcccacct cctggcgtgg ccgcggttca
     4141 cgctgggccg ggggagtgtt gtccgcagcg gcgggttttg ccgttggcat gttgcggctt
     4201 aagcgtttgc gggcgggggc aaaaccgtcg ctggaggggg tgtcggtgga ggcgggttta
     4261 accgcttcta actgtacggc cggagttttg gcggcggggc ggggggcatc cgggcggcgg
     4321 ttttggggtt ttactttggg cttatgggct ttgggtgcgc ctttaagacc tttgctctcg
     4381 ggtaccgcgt gctcaggttc aaagcctttg atggtgtcgc gggtgagctt gcggtcgatg
     4441 agtcgttcaa tgtcccacag ctggtgaaat tcatcggcgc tcaccaggga gatcgcccgg
     4501 cctttactgc ccgcccggcc cgtgcggccg atgcggtgca catagtcctc cggcacattg
     4561 ggcaggtcaa aatttaccac ttggggtagc tcagctatat ccaggccgcg ggcggctatg
     4621 tcggtggcca ccagtatatt cactcggccc cgcttgaagt cttccagggc ttttagccgc
     4681 gcggcctggc ttttgttgcc gtggatggcg ctggagcgga tgtcttcttt gttcaaaaag
     4741 gcggcgagtt tattggcgcc gtgtttggtg cgggtaaaca ctagggcctg gcgccaatcg
     4801 cggctggtga tgaggtggct gagcagttcg gccttgcggg ttttatccac cgggtgaatc
     4861 cactggtcaa ccgtctccgc tgtggagttt ttcgggctga cgttgatttc caccggctca
     4921 cttaaaatac ccagagccag ttggcgaatg tcgtcag
//
LOCUS       DAOQGS010000300         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8935_length_4930_cov_4.714667, whole genome shotgun sequence.
ACCESSION   DAOQGS010000300 DAOQGS010000000
VERSION     DAOQGS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8935_length_4930_cov_4.714667"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="PKZ52_11515"
     CDS             <1..1400
                     /locus_tag="PKZ52_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNG60443.1"
                     /translation="NIDTVYPEHSFNPHQAPPEKQSAAHAAAYMSREDSHAGKSQLLA
                     IDSREHALQAIQTIDAQGEGFGPSKFDDPSHQEFSHYFKFLTLQSQIEGYDPNSEHLP
                     KHPQPPAPAKHLVSTADLATVVFNFPDNPVAASYLPGQAELANVVSGLYQYMLIMTES
                     IFLQPPHNQKRYFNQSLHRSMIWILDKIIQQVRTISFNSGQVNYNLAPTFENINLGHR
                     HQAFNNLNSVCKNFRAQFGNEPWYTATDLDYYIAMIPTLPDVSMFWPDVAKPQLEKFK
                     GVPKFPANPPASVGKDEARHACMGLNHCKGQGRTRDNNCAGQGYCSTALEYNYADPSQ
                     PNISDHTCHVKNNCAGQGGCGLYGTAQEQNQPGHNDCATLGSCATPINAERFSTDGPN
                     RGKGVWTRARRVFEEKTWPTLRKENPSLPKEAPAVPHPELFSNGPTMEWIETYSDQGM
                     TACGASGMSGANSCA"
     gene            1412..2353
                     /locus_tag="PKZ52_11520"
     CDS             1412..2353
                     /locus_tag="PKZ52_11520"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNG60444.1"
                     /translation="MPIDRGHYQLGLGVGLRNVHFDHILRELPAVDWFEAISENFMDS
                     GGRPRAVLRQIAEHYPLVLHGVSLSIGSTDPVNRDYVRRLKALADEIQPRWVSDHLCW
                     TGVLGLNSHDLLPLPLNEEVLRHLIPRIHQVQDMLGRPLVLENPSTYLRFRNSTISEP
                     QFLRALCAATECRLLLDVNNVYVSCFNAGTDPQDYLAEFPFDAVVQLHLAGHQHCGTH
                     MVDTHDGPVCDEVWRLFHTAWHRSKGAATLLEWDGDVPPFALLHAEVLRAREWMSLNE
                     TQPTTQVPAAQRRDQPSTPIGFMVPDVMASSRLYEGV"
     gene            2356..3213
                     /locus_tag="PKZ52_11525"
     CDS             2356..3213
                     /locus_tag="PKZ52_11525"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNG60445.1"
                     /translation="MDKANLAGLQQWFLNALRDPSALDAMSVTNNLLPAANINASACL
                     AIYQRSYVLRLRQCLGDQFPATRHALGEHLFNDFVDCYLKDCPSTSYTLYDLGKRFSE
                     WLEHSRPDRALPEEQRESWIDFMVDLSAYESALFRLFDAPGHEGGEWPQATVADDNLI
                     LQPYLTLATYRYPVAWYYHEIRAGRCPSFPPQATSQVVMLRRDYQTATFPVTAVQFRF
                     LEKMRDGVPISQVLDDIASWTKQPLAQVIHSWRTEVRRPWIAAGFFIECTLLTRRQTQ
                     AEESRVSLD"
     gene            complement(3300..>4930)
                     /locus_tag="PKZ52_11530"
     CDS             complement(3300..>4930)
                     /locus_tag="PKZ52_11530"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNG60446.1"
                     /translation="VLTRRAAAIETLGATTVLCTDKTGTLTHNRMTLMALVSGAKPLL
                     AASGQALSATERSLLEAALLACPKAPSDPMDVAIHRLAHDLGIDASGELLQGFGLRPE
                     LFATTQILRLAHEPHPQAFSKGALEAIALLCRLSADELGALRAQADTLAAEGMRVLAV
                     GRAVSGAAMPASPGELEFSCLGLLAFADPLRETVPAAVAECQSAGIRVVMITGDYPTT
                     ARAIARQAGLLPSDVLSGEELERLTDVQLAERVKTISIFARIRPQQKLRLVQCLKSNG
                     EIVAMTGDGVNDAPAIKAAHIGIAMGGRGTDVAREASSIVLLDDDFGSIVKTIRLGRR
                     IYDNLRKAIEYIVAVHIPIAGLALLPLLMGLPLILTPIHIAFLEMVIDPACSVVFEAE
                     EAEDDVMQRPPRNPQTPLLIGQRISWALLQGFVALALLGGLLAAGVYRQMPENDLRAW
                     VFSTLVLFNMGLILINRSFQASLVTAFVRPNRSLWILFSAVGATLAIAVAWPPAQRLF
                     NFGQFHLSEFLICLGLGAACLLALEGVKKFWFRV"
BASE COUNT         1119 a         1329 c         1382 g         1100 t
ORIGIN      
        1 ataatattga tacggtttat cccgaacaca gttttaaccc gcaccaagcg ccgccggaga
       61 aacagtcggc ggcccatgcc gccgcctaca tgagccgcga ggatagccat gcaggcaaat
      121 cccaactgct ggcgattgat tcgcgcgaac acgccctgca ggcgatccaa actattgatg
      181 cgcaggggga aggttttggc ccgagcaaat ttgatgatcc atcccatcag gaattctcac
      241 attactttaa atttctaacc ctgcaatcgc agattgaagg ttatgaccct aatagcgaac
      301 atttgcccaa acatccgcaa ccaccagcgc cagcaaaaca tttggtgagc actgccgatc
      361 tagccacggt ggtttttaat tttccagata accctgttgc tgcatcctac ctgccggggc
      421 aagccgagct tgccaatgta gtcagtggct tgtatcaata catgctaatt atgacggaga
      481 gtattttttt acagccgccc cataatcaaa agcgctattt caatcaatcc ttacaccgct
      541 cgatgatttg gattctcgat aaaataattc agcaagtgcg cacgatttct tttaatagcg
      601 gccaggtgaa ctacaacctg gcacccacgt ttgaaaatat aaatttgggg catcgccatc
      661 aggcatttaa caaccttaat agtgtgtgta aaaacttccg cgcacaattt ggcaatgagc
      721 cttggtatac cgcaacggat ttggattatt acattgcgat gatccccaca ttgccagatg
      781 tcagtatgtt ctggccggat gtggcaaagc cacaattgga aaaatttaaa ggtgtgccta
      841 aatttcccgc caatccaccg gcatcggtgg gtaaggatga agcgcgccac gcctgtatgg
      901 gtttgaacca ctgtaaaggc cagggccgca cccgcgataa taattgcgcc gggcagggat
      961 attgctccac cgcgctggaa tacaattatg ccgatccatc acaaccgaat atttcggatc
     1021 acacctgcca tgtaaaaaat aattgtgcgg ggcagggtgg ctgcgggctt tatggtacgg
     1081 ctcaggagca aaaccagcct ggacataacg actgcgcaac actgggttcc tgtgcaaccc
     1141 ctatcaacgc cgagcgattt agtaccgatg gccccaatcg cggcaaaggt gtatggacgc
     1201 gcgcgcgacg cgtatttgag gaaaaaacct ggccgacact gcgcaaggaa aatccgtccc
     1261 tgccaaaaga ggcaccggca gtgcctcatc ctgaactctt cagcaatggc ccaaccatgg
     1321 agtggattga aacctacagt gatcagggca tgaccgcctg tggtgccagt ggcatgagcg
     1381 gtgccaatag ctgcgcgtga tggagttggc gatgcctata gatcgaggtc attatcaact
     1441 tgggttgggt gtgggtttgc gcaatgtgca ttttgatcac atcctgcgtg agctgcctgc
     1501 ggtggattgg tttgaggcca tttcagaaaa ctttatggat tccggtggca ggccccgcgc
     1561 tgtgctgcgg cagattgccg agcactaccc gctggtgctg cacggcgtat cgctgtcgat
     1621 tggcagtacg gacccagtca accgcgatta tgtgcggcgc cttaaagcct tagccgatga
     1681 aattcagccg cgctgggtca gcgatcatct gtgctggact ggcgtgctgg ggctcaacag
     1741 tcacgatcta ttgcccttac cgctcaatga agaggtgctc cggcatctga tcccccgcat
     1801 ccatcaggtg caggacatgc ttgggcgtcc tttggtgttg gaaaatccca gcacctattt
     1861 acgctttcgc aattccacaa taagcgaacc gcaatttttg cgcgcactct gcgccgcgac
     1921 ggagtgtcgg ctgttgttgg atgttaataa tgtgtatgta agctgcttta acgcaggtac
     1981 tgacccacag gattacctgg ctgaatttcc atttgatgct gtagtacaac tgcaccttgc
     2041 cggccaccaa cactgtggca cccatatggt ggatacccac gatggcccgg tgtgtgatga
     2101 ggtgtggcgg ctatttcaca ccgcttggca cagatccaag ggggccgcca ccctattgga
     2161 gtgggatggt gatgtgccgc cctttgcgct attacacgcg gaagtattgc gtgcccgtga
     2221 atggatgagc ctcaatgaaa cgcagccgac gacacaggtg cctgccgccc agcgcaggga
     2281 tcagccctcc acacccatcg gttttatggt gccggatgta atggccagca gccgcttgta
     2341 tgagggggtt taaggatgga caaagccaac ttggcgggac tgcaacagtg gtttttaaat
     2401 gcgctgcgcg atccttcggc gctggatgca atgagtgtta caaataacct gttaccggct
     2461 gcaaatatta atgcctcagc gtgcctcgct atttaccagc gcagttatgt actgcgcttg
     2521 cgccagtgtc tgggggatca atttccagcg acgcgccacg ccttagggga gcatttattt
     2581 aatgattttg ttgattgtta cctgaaggat tgcccctcca cgagttacac gctttacgat
     2641 ttgggcaagc ggttttcaga gtggttggaa cattcgcgcc cggatcgcgc actgccggag
     2701 gaacagcggg agagctggat cgattttatg gtggatttat cggcctatga aagcgcactg
     2761 tttcgtctct tcgatgcccc tggccatgaa ggcggcgaat ggccgcaggc aacggtagcg
     2821 gatgacaatt taattctgca gccttacctt acactcgcca cctaccgtta ccctgtggct
     2881 tggtattacc acgaaattcg cgcgggccga tgcccaagct ttccgccgca ggccacctca
     2941 caggtggtga tgctgcgccg cgattatcag acggctacat ttccggtgac tgcagtgcaa
     3001 tttagatttt tagaaaaaat gcgcgacggc gttccaatca gccaggtatt ggatgatatt
     3061 gcgagttgga ccaagcaacc cttggcgcag gtaattcatt cttggcgcac agaagtcagg
     3121 cgcccatgga ttgcagccgg tttttttatc gagtgcactt tattaacccg gcggcaaacc
     3181 caagcagaag aatcgagagt gtcgctggat taaggcgaat ataagggctt ttttaataat
     3241 tgggcgcaac ccaaaactcc tcgccgccgg gttaataaaa gtgcagttgt ggcgatactc
     3301 taaacacgaa accagaattt tttaacccct tccagcgcca ataggcatgc cgccccaagc
     3361 cccaggcata ttaaaaattc actgaggtga aattggccaa aattaaatag cctctgggcg
     3421 ggtggccagg ccacggcaat tgccagggtt gcacccacgg cgctaaataa aatccataag
     3481 gagcgattgg gccgcacaaa agcggttacc agggaggctt gaaacgagcg gttaattaaa
     3541 attagcccca tgttaaataa caccagggtg ctaaataccc aggcccgcag gtcgttttcc
     3601 ggcatctgtc tatacacacc tgcggccagt aaaccaccca gtagtgccag ggccacaaaa
     3661 ccctgtagca aggcccagct gatgcgttgg ccgattaata aaggtgtttg cgggtttctc
     3721 ggcggccgct gcattacatc gtcttctgcc tcttccgctt caaataccac cgaacaggcc
     3781 gggtcgatca ccatttccaa aaatgcgata tgaatcggcg tgaggatcag cggcagcccc
     3841 attaataacg gcaatagcgc caggcccgct atgggaatat gcaccgccac tatgtattcg
     3901 atggccttgc gcaaattgtc gtaaatgcgc ctgcctaggc gaatggtttt gacgatagag
     3961 ccgaagtcat catccagcag cacaatggat gaggcctccc gagccacatc cgtgccgcgc
     4021 ccgcccatgg cgatgcctat gtgggcggct ttgatggccg gggcatcgtt caccccatcg
     4081 ccggtcatgg cgacgatttc accgttactc ttcaggcatt gcaccaggcg cagtttttgt
     4141 tgcgggcgaa tgcgggcgaa gatggagatg gttttcaccc gctcggccag ctgcacatcc
     4201 gttaggcgct ccagctcttc gccgctgagc acatcgctgg gcagcaggcc cgcctgtcgc
     4261 gctatggctc gggcggtggt ggggtaatcg ccggtaatca tcaccacccg gataccggcg
     4321 gactggcact cggccaccgc ggcgggcacg gtttcccgca gcgggtcggc gaaggcgagc
     4381 aggcccaaac aactaaattc aagctcgcct ggggatgcag gcattgcagc ccccgacacg
     4441 gcccggccca cggctaacac ccgcataccc tcggcggcca gggtgtcggc ttgggcccta
     4501 agggcgccga gttcatcggc tgacaggcgg cacagcaggg caatggcttc tagggcgcct
     4561 ttgctaaacg cctgggggtg aggctcatgg gcgaggcgca ggatttgggt ggtggcgaat
     4621 aactccgggc gcaggccaaa gccttgcaac agttccccgc tggcgtcgat gccgaggtcg
     4681 tgggccaagc ggtggatggc cacatccata gggtcgctgg gggccttagg gcaggcgagt
     4741 agggcggctt caagaaggct tcgttctgtg gcgctcaggg cctgcccgct tgcggccagc
     4801 aagggcttag cgccgctcac cagggccatt agggtcatgc ggttgtgggt gagggtgccg
     4861 gttttatccg tgcacaagac ggtggtggcg ccgagggttt cgatggcggc ggcgcggcgg
     4921 gtgagtaccc
//
LOCUS       DAOQGS010000301         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_8996_length_4905_cov_4.242887, whole genome shotgun sequence.
ACCESSION   DAOQGS010000301 DAOQGS010000000
VERSION     DAOQGS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8996_length_4905_cov_4.242887"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="rsxC"
                     /locus_tag="PKZ52_11535"
     CDS             <1..874
                     /gene="rsxC"
                     /locus_tag="PKZ52_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNG60447.1"
                     /translation="VVIGIEDNKPAAIAAMRQAAAPHGAIEVVVVPSRYPMGSAKQLT
                     QAVTGLEVPAGGRSNDVGVLVHNVATIYAIQQALRFGRPLISRVLTVGGDCVGKPQNV
                     EALIGTPLSHLVQTCGGITGKPARLLMGGPMMGQALPTLDVPLLKGCSGVLALSQKEV
                     NAEAPSPCIRCSRCVSACPMGLVPLEMAKFAKVDNFDQAQEYGLRDCILCGSCSYVCP
                     SHIPLVQYFEYAKGEIYTRKNDQKKMEYTVRLTNERNERLEREAAAKAAAKSQKKAKR
                     GSSEAAATSTETAE"
     gene            906..1997
                     /locus_tag="PKZ52_11540"
     CDS             906..1997
                     /locus_tag="PKZ52_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNG60448.1"
                     /translation="MTARSLPVAGAAPHAHDRASVDRIMLQVCFALAPATAFGFYLFG
                     WPAVFLWLFTCAGALITEALCLWGQGQSIARLKDSSALLTGWLLALSLPPWAPWWIGI
                     GGAAFAIGIGKQVYGGLGQNLFNPAMLARVALLISFPVQMSTWANITPMGSLLAPGIW
                     DGLGITLGLNSIPDGATGATILGEAKANYTTSLPIGDMLAQHFSLSQAFVGHTRGSLG
                     ETSELLILAGGLWLMYLRIISWHIPVAFLASLAFFSGLFHHANPARYAAASFHLTSGG
                     IAMGAFFIATDYVTSPASNKGKLLFGIGCGFMVFVIRAYSGFPEAVAFAVLFMNALTP
                     LIDRISKPRSYGRDGRGRPLKVPSARKEP"
     gene            2001..2639
                     /gene="rsxG"
                     /locus_tag="PKZ52_11545"
     CDS             2001..2639
                     /gene="rsxG"
                     /locus_tag="PKZ52_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNG60449.1"
                     /translation="MTSVAQAGFRSRLPYQAGLLGGVCCLISLLLIIGNSKTSVIIEE
                     QVNQDKLAMMADVLPSNLYDNNPIKESTTISDSPIMSNPITVMKASKSGQLSGAVLQV
                     TIQGWGGKIQLIMGVNAQGEINGVRIVSHKETPGLADKIEVEKSEWIKSFNGKSLANT
                     SEKNWAVKKDGGEFDQFAGATITPRAVVKGVHQGMQFYQEWSKTQSSGMQTP"
     gene            2667..3356
                     /locus_tag="PKZ52_11550"
     CDS             2667..3356
                     /locus_tag="PKZ52_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNG60450.1"
                     /translation="MSDVNYGRIFRDGIWDNNVSLGQILALCPLMAVTTSATNGLGMG
                     IATTAVLILTNAMISSVRGIISPQVRIPVMIVLIAAVVTVVDMGINAWMHSLYKVLGL
                     FIALIVTNCAIFGRAEAFAIKNPILPSMADALAMGIGFSLVLIAVGGVREILGSGTLF
                     AHASLLLGAHFAWLEMVIIPKFQGILLAILPPGAFLTLGFLIAAKRAIDNYVSRKKAQ
                     TPPSPLVVLQT"
     gene            3382..3639
                     /locus_tag="PKZ52_11555"
     CDS             3382..3639
                     /locus_tag="PKZ52_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNG60451.1"
                     /translation="MKVSIAYAAENSPYWADFNVDDETTVATAIEKSGILKQFPEINL
                     RQAKIGIFGKFTKPDALVQPGDRIEIYQPIIRVLDDDDDDD"
     gene            3623..4717
                     /locus_tag="PKZ52_11560"
     CDS             3623..4717
                     /locus_tag="PKZ52_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG60452.1"
                     /translation="MMTTINPQRLIQLITAGCILALCACSRDTTTSLTGTNLATTYLI
                     EGAAQGTRYHITYVAPVNFSSTQQAALSAAISAELARLDKAISNYRPDSEIEQFNSQI
                     TTEPVEVSAEIVKLIEIARSVHKASNGCYDLTIKPLFDLWGFKKNVFTPPSDEALAQT
                     LQLVGMEKLETLDKNHLRKKIPNLRVDLSSIGQGYSISTLAAILEAQAITNYIVEIGG
                     ELKARGQKPGNKPWRVALEKPLSNEQKIEKIAVFNSIEPMSMMPSGTYHHYFDADGKR
                     YSHILNARTGKPITHTSASATVLNSDPTLADAWSTALLCLGSQEGIPIANAQGIAALY
                     IDQRSDGFSEQPTQPLINLSTVSFENAKGD"
BASE COUNT         1208 a         1439 c         1186 g         1072 t
ORIGIN      
        1 cgttgtgatc ggcattgaag acaacaagcc cgcagccata gccgccatgc gccaagccgc
       61 cgccccccac ggcgccattg aagtggtggt ggtgccctcc cgctacccta tgggttcagc
      121 caaacaactg actcaagcgg taaccggttt ggaagtaccc gccggtggcc gcagcaacga
      181 tgtgggcgtg ctggtgcaca acgtggccac catctacgcc attcaacagg ccctgcgctt
      241 tggccgcccc ttgatctctc gggtgctcac cgtgggcggc gactgtgtgg gcaagccgca
      301 aaatgtagag gccctcattg gcactccctt aagccaccta gtgcaaacct gcggcggcat
      361 caccggtaaa cccgcccgct tactgatggg cggccccatg atgggccaag ccctacccac
      421 cctggatgtg ccactgctta agggctgctc gggtgtactg gcgttgagcc aaaaagaagt
      481 caacgcggag gcgccctccc cctgcatccg ctgcagccgc tgcgtcagcg cctgccccat
      541 ggggctggtg ccgctggaaa tggccaagtt cgccaaggtg gacaatttcg atcaagccca
      601 ggaatacggc ctgcgggact gtattttgtg cggctcctgc tcctacgtgt gcccctccca
      661 tattcccctg gtgcagtact ttgaatacgc caagggtgaa atttacacac gcaaaaatga
      721 ccagaaaaaa atggagtaca ccgtgcgcct caccaacgag cgcaacgaac gcctagagcg
      781 ggaagcggcg gccaaagcgg ccgccaagag ccagaaaaaa gccaagcgcg gcagcagtga
      841 agcggccgca accagtaccg aaaccgctga ataaacagct taacttcact gaccgagatg
      901 cacctatgac cgcccgaagc cttccagtgg caggcgccgc cccccacgcc cacgaccgcg
      961 ccagcgtcga tcgcatcatg ctgcaggtgt gctttgcact ggcacctgca actgccttcg
     1021 ggttttattt atttggttgg ccagcggtat ttttatggtt gttcacctgt gccggcgccc
     1081 tcatcactga ggccctttgc ctatggggcc aaggccagag catcgcccgc ttaaaggatt
     1141 cctccgccct gctaaccggc tggctgctcg ccctcagcct gcccccctgg gcaccctggt
     1201 ggattgggat tggcggcgca gcttttgcca tcggcatagg caaacaggtg tacggcggcc
     1261 tcggccaaaa ccttttcaac cccgccatgc tcgcccgcgt agccctattg atttccttcc
     1321 cagtgcagat gtccacctgg gccaacataa cccccatggg cagcttattg gcccccggta
     1381 tatgggacgg cctgggtata actctgggcc ttaacagcat ccccgatggc gccaccggcg
     1441 ccaccatatt gggggaggcc aaagccaact acaccaccag cctgcccatc ggcgacatgc
     1501 tggcccagca cttctcctta agccaagcct ttgtgggcca cacccgcggc agcctggggg
     1561 aaacctccga actattaatt ctcgctggcg gcctgtggct tatgtacctg cgcattatca
     1621 gctggcacat accggtcgca tttttggcca gcctggcctt ttttagtggc ttatttcacc
     1681 acgccaaccc tgcccgctac gccgccgcca gttttcacct cacctccggc ggcattgcca
     1741 tgggcgcctt ttttatcgcc actgattacg tcacctcacc ggctagcaat aaaggcaaat
     1801 tattatttgg tatcggctgc ggctttatgg tgtttgtgat tcgcgcctac agcggctttc
     1861 ccgaagcggt ggcctttgcg gtgttattta tgaatgcgct gacgccttta attgaccgca
     1921 tatccaaacc gcgctcctac ggccgcgacg gccgcggccg cccgctcaaa gtaccctctg
     1981 cccgtaagga gccataattt atgaccagtg ttgcacaagc gggttttcgc tcgcgcctac
     2041 cttaccaagc cggtttatta ggcggcgtct gttgtttaat tagtttgttg ttaattatcg
     2101 gcaacagtaa aaccagcgta atcattgaag aacaagtcaa ccaggataaa ttagccatga
     2161 tggcggacgt gttgccatcc aacctctacg ataacaaccc cattaaagaa agcaccacca
     2221 tcagcgacag ccccattatg agcaacccca tcaccgtaat gaaggccagt aaaagtggcc
     2281 aattaagcgg cgcggtatta caagtgacta ttcaaggttg gggcggcaaa attcaattaa
     2341 ttatgggcgt gaatgcccag ggtgaaatca acggcgtgcg cattgtcagc cacaaagaaa
     2401 cgccgggcct ggccgacaaa attgaagtgg aaaaaagcga gtggattaaa agctttaatg
     2461 gcaaatccct cgctaacacg tccgaaaaaa actgggcagt gaaaaaagac ggcggcgaat
     2521 ttgaccagtt tgccggtgcc accattacac cccgcgcagt agtaaaaggc gtgcaccagg
     2581 gtatgcagtt ttatcaagag tggtcaaaaa cccaatcaag cggtatgcaa accccctaag
     2641 accagcgaca aaagtaggta ttaactatga gcgatgtaaa ttacgggcga atttttcgcg
     2701 atggcatttg ggataacaat gtctccctag ggcaaattct cgccctttgc cctctaatgg
     2761 ccgttaccac cagcgccacc aatgggctgg gcatggggat agccacaact gcggtattaa
     2821 ttcttaccaa tgcgatgatt tcatctgtgc gcggcattat ttcaccccag gtgcgcatcc
     2881 cagtgatgat tgtactgata gccgctgtgg tgacagtggt agatatgggc attaacgcct
     2941 ggatgcactc gctgtataaa gtactgggtt tatttatagc gctgattgta accaattgcg
     3001 ctatttttgg ccgcgccgaa gccttcgcca ttaaaaatcc cattttgccc tctatggcag
     3061 atgcactagc catgggtatt ggcttttcct tggtgcttat cgctgtgggt ggcgtgcggg
     3121 aaattctcgg cagcggcaca ttattcgccc acgcatcgtt attactcggc gcccatttcg
     3181 cctggctgga aatggtcatc attcctaaat tccaaggaat tttgctggcg attttacctc
     3241 ccggcgcctt tttaaccctg ggctttttaa tcgctgccaa acgggctatt gataattacg
     3301 tcagccgcaa aaaagcccaa acgcccccct cccctttagt ggttttgcaa acctaatctg
     3361 catagctttt aggaggcccc tatgaaagtc agcatcgcct atgccgccga aaactccccc
     3421 tactgggcgg attttaatgt ggatgatgaa accaccgtcg ccacagccat tgaaaaatcg
     3481 ggcatactta aacaattccc ggaaattaat ctcaggcagg caaaaattgg catttttggc
     3541 aaattcacta aacccgatgc cctggtacaa ccgggggatc gcatcgaaat ttatcagccg
     3601 atcatccgcg tgctggatga cgatgatgac gacgattaac ccccagcgat taattcaact
     3661 catcaccgca ggctgcattt tggccttatg cgcctgcagc cgcgatacca ctaccagttt
     3721 aaccggcacc aatttagcca ccacctattt aattgaaggc gctgcccaag gcactcgcta
     3781 ccacattacc tacgtggcgc cggttaattt cagcagcaca caacaggccg cgctctcagc
     3841 ggccattagc gctgaattag cgcgcttaga taaagccatt tccaattacc ggcccgattc
     3901 cgagatagaa caatttaata gccaaatcac caccgagccg gtggaagtgt ccgctgaaat
     3961 tgttaagctc attgaaattg cccgcagcgt gcataaagcc agcaacggct gttacgatct
     4021 caccataaaa ccgctgtttg atttatgggg ttttaaaaaa aatgtcttta cacccccgag
     4081 cgatgaagcc ctcgcgcaaa cgctgcaact cgtcggcatg gaaaaattag agaccttgga
     4141 taaaaaccac ctgcgcaaaa aaatccccaa cctgcgggtt gatttatcct ctatcggcca
     4201 gggctacagc atcagtactc tagccgccat tttagaggcc caagccatca ccaattacat
     4261 tgttgaaatc ggcggcgaat taaaagcccg cggccagaaa cccggcaata aaccctggcg
     4321 cgtcgccttg gaaaaaccat taagcaacga acaaaaaatt gaaaaaatag cggtgtttaa
     4381 tagcatcgag cctatgtcta tgatgccttc cggcacctat caccattatt ttgatgccga
     4441 cggcaaacgc tacagccaca tactcaacgc ccgcaccggc aaacccatca cccacaccag
     4501 cgcctcggcc accgtgttaa actcagaccc aaccctcgcc gatgcctggt ccactgcact
     4561 tttgtgttta ggcagccaag agggcatccc catcgctaat gcccagggca ttgccgcctt
     4621 gtatatcgat cagcgcagcg atggttttag cgagcaacct acccaacccc taatcaattt
     4681 atcaaccgta agttttgaaa atgccaaagg cgattaaacc cctgcagcaa acctcacaga
     4741 gcaagagctg taacaaaccg tacaataacc gtcttaaata ccacaaaacg acgctcagat
     4801 agggcgataa aatttaccgc aggcaggcgc gagggcaaat taagttactt gggaacctct
     4861 gaaaaatgta ggcggcgaag cgctggctag gcgaatttgc caaaa
//
LOCUS       DAOQGS010000302         4900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9007_length_4900_cov_4.639009, whole genome shotgun sequence.
ACCESSION   DAOQGS010000302 DAOQGS010000000
VERSION     DAOQGS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9007_length_4900_cov_4.639009"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKZ52_11565"
     CDS             complement(<1..581)
                     /locus_tag="PKZ52_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG60453.1"
                     /translation="MATNHNTSLFKLSPLNAKRWQHFKHNRRAFISLWLFLLLALVSA
                     GAELVANERPLLIYSEGRWLFPVLVDYTEQDLGGDFATAANYQDPFVQDLIAQKGWML
                     KALVPYSYNTHIHQLPTPAPSPPTAHNWLGTDDQARDVFARVLYGTRTSIAFALCLAL
                     CGAALGVLVGAVQGYYGGWVDLLGQRFLEVWSSL"
     gene            complement(589..1641)
                     /gene="yejB"
                     /locus_tag="PKZ52_11570"
     CDS             complement(589..1641)
                     /gene="yejB"
                     /locus_tag="PKZ52_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HNG60454.1"
                     /translation="MGQYILRRLLLMVPTLFGILLINFAVVQFTPGGPVEQMLYRLKN
                     PDHTNASAQADIQQSGQARGIPPEVIKKIEALYGFDQPAYVRFGQMVVRYLQGDFGES
                     FYRNAKVTDLIAEKLPTSISLGLWSTLLIYFISIPLGIYKAVRHGSRFDVASSFMLSI
                     LYALPGFLLAMVLIVLLAGGSYWQVFPLRGLTSEGFSDFTLWQQITDYFWHLALPLTA
                     YTIGGFATLTALTKNSFLDEINKQYVYTARAKGLTERQVLYGHVFRNSMLIIIAGFPA
                     AFVGMFFTGSLLIEVMFSLDGLGLLSFEAVTQRDYPVFFGTLFIFTLLGLVVKLLSDL
                     VYVWVDPRIHFQQQMG"
     gene            complement(1644..3521)
                     /locus_tag="PKZ52_11575"
     CDS             complement(1644..3521)
                     /locus_tag="PKZ52_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNG60455.1"
                     /translation="MNTRRLLYPLVALCCSFMIALATQAADQTAKDAPSSPKTKITHA
                     LTLMGEPKYPANFERFEYTSAKAQKGGSVKLAGYGTFDSLNAFITKGTPDVYLGLLYD
                     TLTQQSLDEPFTEYGLVAEKIELAEDRSFVIFHINPKAKFNDGKAITAEDVVFTFNTL
                     ITKGAPQYATLYAEVKSVQALDAARVKFDFKTTTNRELPLIVGQLPVLPKHFWQNKDF
                     AVASLDIPLGSGPYKIKKVDAGRSLVYERVANYWAADLPINRGMYNFDQLTLDYYRDD
                     TVIVEALKARQLDYRWERVAKTWATAYDTPAVKSGLLKKQLIDDKTPTGMSALIFNLR
                     KPLFDNIYLRQAFNYAFDFEWTNKTVFFDSYERIQSYFSNSELASAGLPSGRELEILT
                     KYRDKLPEAVFAQPFANPKTDGSGNNRDNMLKAQALLKQGGFELKEGKLIDPKTQKPL
                     VLELVTHDALFERIANPWIQNLKRLGIEVNLRVVDVSQYINRVNSFNFDLTVVPYSQG
                     LNPGNEQREYWGSAAASIEGSSNYAGIKNPIIDELVDLVIRAPDRPELIAATRALDRV
                     LLNQHYMVSLYTYTKHRLVYWDKFGQPEVAPAYDNYYRAGFMTRWIDKDKEAKLNASK
                     P"
     gene            3616..>4900
                     /locus_tag="PKZ52_11580"
     CDS             3616..>4900
                     /locus_tag="PKZ52_11580"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HNG60456.1"
                     /translation="MAALKTLQGQPKHQAPLPIHPKIRPPMNWNEEILNSLIWLIKTF
                     LISGIGFVVVFGAVLKFTQAGRDFLRLSGGYFNWQRSAKPLLLLCGVIVLALVSVRLS
                     VLISQASNGFYTGLQELNPEAFWTNFGIWFLIAIGFVIRALVAYYVKSALTIDWRIWL
                     TESLITRWLHNQNYYRSHFAKAQMDNPDQRIQQDVESFISLSLSLSVGLLEACVSLFT
                     FTILLWTLSGTLALFGLEIPRAMVILAYVYVFIATGIAIWLGRPLINLNFLNEKFNAN
                     FRYALIRLREYGESIAFYRGEAREQKGLFSRFGDVISNSWAIVFRALKFDGFNISVNQ
                     VASVFPLIIQAPRLFAKEIKLGDMMQSARAFSEVQEALSFFRLSYDNFANYRAVMIRL
                     MGFLQVVDEAENLPRIEAAHQGHNLTLAKFSALKPD"
BASE COUNT         1309 a         1214 c         1156 g         1221 t
ORIGIN      
        1 agagatgacc agacctctaa aaaacgctgg cctaataaat ctacccagcc gccgtaatag
       61 ccttgaacgg cccccaccaa aacccccaac gccgcgccgc agagcgctaa acacaaagcg
      121 aaggcaatgg aggtgcgggt gccatataac acgcgggcaa acacgtcgcg ggcttgatcg
      181 tccgtgccca gccaattgtg cgccgtcggt ggcgatggcg ccggggtggg caattggtga
      241 atatgtgtgt tgtaggaata aggcaccaag gctttcagca tccagccttt ttgcgcaatt
      301 aaatcttgca caaaagggtc ttgataatta gcggcagtgg caaaatcgcc gcctaaatcc
      361 tgctcggtat aatccaccaa tactgggaat aaccagcgcc cttcgctata aattaataat
      421 ggccgctcat tggccactaa ttccgccccg gcggaaacca gcgccaatag caaaaacaac
      481 cataaactga taaaggcgcg gcggttgtgt ttaaagtgtt gccagcgttt agcgttcagg
      541 gggcttagct taaacaggct cgtgttatgg ttcgttgcca tcgcgtgttt agcccatctg
      601 ttgttgaaaa tgaatgcgcg ggtccaccca cacatacacc aaatccgaca gcagcttcac
      661 caccaacccc agtaaagtaa aaataaataa ggtgccaaaa aataccgggt agtcccgctg
      721 ggtaaccgct tcaaaactca acagtcccaa gccatccaaa gaaaacatca cttcaatcaa
      781 caaagagccg gtaaaaaaca tacccacaaa tgccgccgga aagcccgcaa taataatcaa
      841 catagaattg cgaaatacgt ggccgtataa cacctgccgc tcggttaagc ctttggcccg
      901 cgcggtgtat acatattgtt tattaatttc atctaaaaaa gaatttttcg tcagcgccgt
      961 aagtgtggca aaaccgccga tagtgtaagc agtaaggggc agggctaaat gccagaaata
     1021 atccgtaatt tgctgccaga gggtaaaatc gctaaagcct tctgaggtga ggccccgcaa
     1081 agggaatacc tgccaatagg agccaccggc caataacaca attaacacca tggccagcaa
     1141 aaaacctggc agggcataca aaatggagag cataaaactg ctggccacat caaaacggct
     1201 gccgtggcgc acggctttat aaatacccag cggaatggaa ataaaataaa tcagcaaggt
     1261 gctccacagc cccagggaga tagaggtggg cagtttttcg gcaattaagt ccgtcacctt
     1321 ggcgttgcgg taaaaactct cgccaaaatc accctgcaga taacgcacca ccatttgacc
     1381 aaagcgcaca taggccggtt gatcaaaacc gtataaggct tcgatttttt ttatcacctc
     1441 cggcggaatt ccccgcgcct gcccgctctg ctgaatatcc gcctgggccg aggcattagt
     1501 gtggtcagga ttttttaacc ggtagagcat ctgctccacc ggcccgccgg gggtaaactg
     1561 caccacagca aaattaatca gcaatatgcc aaatagcgtg ggcaccatta gcagcaggcg
     1621 ccgcagaata tactgcccca taactagggc ttgctggcgt taagttttgc ttccttgtcc
     1681 ttgtcgatcc accgggtcat aaaaccagcg cgataataat tatcgtaggc aggtgcaacc
     1741 tccggctggc cgaatttatc ccaataaacc aaacgatgtt tggtataggt atagagcgac
     1801 accatgtagt gctggtttaa taaaactcta tctagcgcgc gtgtagcggc aattaactca
     1861 ggccggtcag gcgcgcgaat taccaaatca accagctcgt caataatggg gtttttgata
     1921 ccggcataat tggagctgcc ctcgatactc gccgctgcgc tgccccaata ttcacgttgc
     1981 tcattacctg gattaaggcc ttgggaataa ggcaccacag taagatcaaa attgaagcta
     2041 tttacgcggt taatgtattg agaaacatct acaacgcgaa gattcacctc gatccctagt
     2101 cgttttaagt tttgaatcca agggttggca atacgctcaa aaagcgcatc gtgagtcaca
     2161 agttccaaaa ccagcggctt ttgagttttt ggatcaatta atttaccctc ttttagctca
     2221 aaaccaccct gctttaacaa ggcctgagct ttgagcatat tatcgcgatt gttgccagaa
     2281 ccatcagttt ttgggttggc aaatggctga gcaaaaacag cttctggtag tttatcgcga
     2341 tatttagtga ggatctcaag ctctcgccca ctgggcaagc cagcagaggc caattctgaa
     2401 ttggaaaaat aactttgaat acgctcgtag gaatcaaaaa aaaccgtttt attggtccac
     2461 tcaaaatcaa aagcataatt aaatgcctgg cgcaaataaa tgttatcgaa cagcggtttg
     2521 cgcaggttaa atatcaacgc cgacattcct gtaggggtct tgtcgtcgat tagctgcttt
     2581 ttgagcaggc cggattttac tgctggggtg tcataggcgg tggcccaggt ttttgcaaca
     2641 cgctcccagc gataatcaag ctggcgggct tttaaggctt ctactattac cgtgtcgtca
     2701 cgataatagt ccagcgtcaa ctgatcaaaa ttatacatac cgcgattaat cggcaaatcg
     2761 gcagcccagt agttggctac ccgctcgtag accagcgaac gccctgcgtc caccttttta
     2821 attttgtaag ggccagaccc caaaggaatg tccaagctcg ccacagcaaa atctttgttt
     2881 tgccaaaagt gcttaggcaa cacaggcagc tggccgacaa ttaacggcag ctctcgattg
     2941 gtagtggttt taaagtcgaa ttttacccgc gcagcatcca acgcctgcac cgatttaact
     3001 tccgcataaa gcgtagcgta ttgcggcgct ccctttgtaa tcagggtatt aaatgtaaaa
     3061 accacatcct cagccgtaat tgccttgcca tcattaaatt ttgccttggg attaatatga
     3121 aagattacaa aactgcggtc ttcagctaat tcaatttttt ccgctaccaa accatattca
     3181 gtaaacggct catctaatga ctgctgggtg agggtgtcgt acaagaggcc caggtagaca
     3241 tcgggtgtac ccttggtaat aaacgcattc aggctatcaa aagtgccgta tccagcgagc
     3301 ttaacactcc cacctttttg cgccttagcc gaggtgtatt caaagcgttc aaaattagcc
     3361 ggatatttag gctcccccat tagggtaagg gcgtgggtga ttttggtttt gggggaactg
     3421 ggtgcgtcct tggcggtctg gtcggcggcc tgtgtggcaa gtgcaatcat aaagctgcaa
     3481 cacagggcca ccaatggata cagcagacgg cgagtattca tagggtaccc ttaaggtcag
     3541 tttaaactgg gcgcagagtg taccctaaag cctgccggtt acaagcggta ggcgaaggcg
     3601 ccgcaaggcc ccataatggc tgcccttaaa acccttcaag ggcaacccaa acaccaagcc
     3661 cctttaccca tccaccccaa aataaggccc cccatgaatt ggaacgaaga aattctcaac
     3721 agcctcattt ggcttattaa aacgtttttg atcagcggca ttggatttgt tgttgtattt
     3781 ggcgccgtgc tgaaatttac ccaggccggg cgagattttt tgaggttgag cggcggctat
     3841 tttaactggc agcgcagcgc aaaacccctg ctgctgctgt gtggcgttat tgtgctggca
     3901 ttggtttcgg tgcgtttatc ggtattaatc tcccaagcca gtaatggctt ttatacaggg
     3961 ctacaggagc ttaacccaga ggcgttttgg accaattttg gcatttggtt tttaatcgcc
     4021 atcggttttg ttattcgcgc tttagtggcc tattacgtaa aaagcgcgct caccatcgac
     4081 tggcgtattt ggttaaccga atccttaatt acccgctggt tgcacaacca aaattattac
     4141 cgcagccatt ttgccaaagc gcaaatggat aacccagatc aacgtattca acaggatgtg
     4201 gaaagtttta ttagcctgag cctgagctta tccgtagggc ttttagaagc ctgtgtttca
     4261 ttattcacct ttacaatttt actttggacc ctttcaggca cccttgccct attcggcttg
     4321 gaaattcccc gcgccatggt aattctggcc tatgtttatg tatttattgc caccggcatt
     4381 gcaatttggc tggggcgccc gctgattaat ttaaattttt taaacgaaaa atttaacgct
     4441 aatttccgat atgcgttgat acgcctgcgc gagtatggcg agagcattgc cttttaccgg
     4501 ggagaagcgc gggagcaaaa aggcctattc agccgctttg gtgatgtcat cagcaatagc
     4561 tgggcgattg tgttccgggc ccttaagttt gatggcttta atatctcggt gaaccaggta
     4621 gcttcggtat ttccactcat tattcaggcg ccgcgcttgt tcgccaaaga aattaaatta
     4681 ggcgatatga tgcaaagcgc ccgcgccttt agtgaggtgc aagaggcttt gtcgtttttc
     4741 cgtttgtcct acgataattt tgccaattac cgcgcggtga tgattcgttt gatgggcttt
     4801 ttgcaggtgg tggacgaggc tgagaaccta ccgcgcatag aggcggccca ccaagggcac
     4861 aatcttaccc tggctaaatt ctccgccctc aaacccgatg
//
LOCUS       DAOQGS010000303         4900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9009_length_4900_cov_4.185965, whole genome shotgun sequence.
ACCESSION   DAOQGS010000303 DAOQGS010000000
VERSION     DAOQGS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9009_length_4900_cov_4.185965"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /gene="hscA"
                     /locus_tag="PKZ52_11585"
     CDS             complement(<1..1803)
                     /gene="hscA"
                     /locus_tag="PKZ52_11585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNG60457.1"
                     /translation="MALLQISEPGLSPAPHARKLAVGIDLGTTNSLVASVRSGTPTAL
                     VDAQGESILPSVVCYSPGQVVVGAEAKRQAAQDPLNTVSSVKRLMGRSVKEVKSLGRH
                     LPYQLSEGENQVPLIHTAGGVKTAVEVSADILKVLAARATEALGGALEGCVITVPAYF
                     DDAQRQATKDAGRLAGLNVLRLLNEPTAAAVAYGLDQAEEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLSTGGDSALGGDDLDHTLAEYFIAQWQLGEDLSPSVQRQLLDLARASKE
                     QLSNQDAVTAVLGERQLPLTRAQFNQLIDPLIDKTLLACRRALRDADVALDEIKNVVM
                     VGGSTRTPRVRERVGALFGREPLVSIDPDKVVALGAAIQADVLVGNKPADELLLLDVI
                     PLSLGLETMGGLVEKIIERNTTIPVAKAQEFTTFKDGQTAMALHVVQGERDLVSHCRS
                     LARFELRNIPPKVAGAAHIVVTFQVDADGLLSVSAQEKSTGVQAAIVVKPSYGLDDES
                     ITRMLKESFAHAKEDMQERALREQKVEAERVIEALVSALDKDGAHLLTAAEQADIAAG
                     LEQLRASHNGLDSDAIRREWPHLLVFGALGMWICG"
     gene            complement(1814..2335)
                     /gene="hscB"
                     /locus_tag="PKZ52_11590"
     CDS             complement(1814..2335)
                     /gene="hscB"
                     /locus_tag="PKZ52_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNG60458.1"
                     /translation="MIQHNYFELFQLPQAFDLDQAALAAAYRKVQQQVHPDKFAGLPA
                     AEQRMAVQFAALVNQAFDTLRHSVKRAQYLLELAGQGQSMQNSSTTDPEFLLQQMQWR
                     ERLADCQDEADPLAALDALRQQAKAAMAVLEGEFGAAYKACNWPEARQLLGKLMFVNK
                     LLADIHQHEDLYL"
     gene            complement(2608..2931)
                     /gene="iscA"
                     /locus_tag="PKZ52_11595"
     CDS             complement(2608..2931)
                     /gene="iscA"
                     /locus_tag="PKZ52_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNG60459.1"
                     /translation="MALTMSQAAVHHVKKHLASRGKGAGIRLGVKTTGCSGLSYVLEF
                     VDAFAEDDVIFPQEGFSLVVDPKSLAYLDGTELDFAKEGLNEGFKFNNPNVKNECGCG
                     ESFHV"
     gene            complement(2962..3345)
                     /gene="iscU"
                     /locus_tag="PKZ52_11600"
     CDS             complement(2962..3345)
                     /gene="iscU"
                     /locus_tag="PKZ52_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNG60460.1"
                     /translation="MAYSEKVIDHYENPRNVGKLDAEANNVGTGMVGAPACGDVMRLQ
                     IQVNEQGIIEDAKFKTYGCGSAIASSSLLTEWVKGKTLDEAAQIKNVDIAEELALPPV
                     KIHCSVLAEDAIRAAIDDLRKKRGE"
     gene            complement(3370..4584)
                     /locus_tag="PKZ52_11605"
     CDS             complement(3370..4584)
                     /locus_tag="PKZ52_11605"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNG60461.1"
                     /translation="MQTPIYFDYSATTPVDPSVAEAMMQCLTREGNFGNPASRSHVFG
                     WKAEEAVEHAREQLAQLINADPREIVWTSGATESDNLAIKGVALFNSKRGKHLITSKI
                     EHKAVLDTCRQLEREGFEVTYLEPTSEGIITPEAVAAAIRPDTTLVSLMHVNNEIGVI
                     NDIAAIGAVCRERHVYFHVDGAQSLGKLPVDMEQLPVDLLSISAHKMYGPKGIGALYV
                     RRKPRVRIEAQIHGGGHERGMRSGTLPTHQIVGLGAAAAVAKARLEADYQHAKTLRER
                     FLAGISDIEQVHINGSLSQSVPHILNVSFAFVEGESLIMSLKDLAVSSGSACTSASLE
                     PSYVLRALGLSDELAHSSLRFGFGRGTTIEQVDFAVEKIHYAVNKLRELSPLWDMYKD
                     GIDLNKVEWAAH"
     gene            complement(4658..>4900)
                     /locus_tag="PKZ52_11610"
     CDS             complement(4658..>4900)
                     /locus_tag="PKZ52_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNG60462.1"
                     /translation="IDAVNESVDATHCGGQGNCQKGEVCLTHYLWSDLSDQIHQFLSG
                     ISLASLVARQDVRRVSLRQDELENATEERITLSPLV"
BASE COUNT         1091 a         1426 c         1277 g         1106 t
ORIGIN      
        1 gccgcagatc cacatgccga gcgcgccgaa caccagcagg tgcggccact cgcggcggat
       61 ggcgtcgcta tccaggccgt tgtgggaggc gcgcagctgc tcaaggcctg cggctatgtc
      121 agcctgttcg gcagcggtca gtaaatgggc gccgtcttta tccagggcgc tcaccagagc
      181 ctctatcacc cgctcggctt ccactttttg ctcccgcagg gcgcgctctt gcatgtcttc
      241 tttggcgtgg gcgaaggact ctttgagcat ccgcgtgatg ctttcatcgt ccaaaccata
      301 ggagggttta accactatgg ccgcctgcac cccggtggat ttctcctggg cgctcacgct
      361 caataagccg tcggcatcca cctgaaaggt caccactatg tgggcagcgc cagccacttt
      421 gggggggata ttgcgcagct caaagcgagc taaggagcga cagtggctca ccagatctcg
      481 ctccccctgc actacatgca gtgccatggc ggtttggcca tctttaaagg tggtgaactc
      541 ttgggccttg gccaccggaa tggtggtgtt gcgttcgata attttctcca ccaagccgcc
      601 catggtttcc aggcccagag agaggggaat aacatccagc agtaggagtt catcggcggg
      661 tttattgccc accagcacat cggcttgaat ggccgcgccg agggcgacga ccttatcagg
      721 gtcgatggag accaacggct cccggccaaa cagcgcgccc acccgctcgc gcacgcgggg
      781 ggtgcgggtg gaaccgccca ccatcaccac atttttaatc tcatccaagg ccacatccgc
      841 atcccgcagg gcgcggcgac aggccagtag ggttttatca attaaggggt cgatcaactg
      901 gttaaactgc gcgcgggtaa gcggtaattg ccgctcgccc aagacagccg tcaccgcatc
      961 ctgattggag agctgttctt tgctggcgcg agccagatcc aacaactgcc gctgcacaga
     1021 aggcgataga tcctccccca gctgccactg cgcaataaaa tactccgcta gggtgtggtc
     1081 caaatcgtcc ccgccgaggg cggagtcgcc gccggtggac aagacctcaa acaccccacg
     1141 gcttaagcgc aggatagaaa tatcaaaggt gccgccgccc aaatcgtaca cggctatcac
     1201 accctcttcc gcttgatcca agccataggc cacggcggcg gcggtgggtt cgttgagcag
     1261 gcgcagaaca ttcaggcccg ctaggcggcc cgcatctttg gttgcctggc gctgggcgtc
     1321 gtcaaaataa gcgggcaccg taatcacaca gccttctaag gcgccgccga gggcttcagt
     1381 ggcgcgggcc gctaggactt ttaaaatatc cgccgagact tctaccgcgg ttttgacacc
     1441 gcctgcggta tgaatcagcg gcacctggtt ttccccctca ctcagttgat agggcaaatg
     1501 gcggccaagg cttttcacct cttttacact gcggcccatc aggcgtttaa ccgagctgac
     1561 ggtattgagc gggtcctggg ccgcctggcg tttggcctct gcgccaacca ccacctgccc
     1621 agggctgtaa cacaccacgg agggcaaaat actctccccc tgcgcgtcca ctaaggctgt
     1681 gggcgtgcca gagcgaacac tggcgaccag ggaattggtg gtgcccaaat cgatacccac
     1741 cgccagtttg cgagcgtggg gtgcggggct taggcccggt tcagaaattt gcaaaagcgc
     1801 catggaataa ccgttagaga taaagatctt cgtgctggtg gatgtcggcc aatagtttgt
     1861 tcacaaacat cagtttgccg aggagttgcc gggcctctgg ccagttgcag gctttatagg
     1921 cagcaccaaa ctcgccttcc aaaaccgcca tggcggcctt cgcctgttgc cttagggcgt
     1981 ccagcgcggc aagcgggtcc gcctcgtctt ggcaatcggc taagcgctct cgccactgca
     2041 tctgctgcaa taaaaactcg gggtcggtgg tggagctgtt ctgcatggac tgcccctgcc
     2101 ccgccaactc cagcaggtac tgtgcccgct tgacgctgtg gcgcagggta tcaaaggcct
     2161 ggttcaccaa tgcggcgaac tgcaccgcca tgcgctgctc tgcggcgggc agacctgcga
     2221 atttatccgg gtgaacctgt tgctgtactt tgcggtaggc ggcggccaaa gccgcctggt
     2281 ctaaatcaaa cgcctggggt aattgaaata actcaaaata attgtgttgg atcacttaag
     2341 ggttccacaa aagcggtttg gcctgtttcg aaggttttag cgcgcaggct gttagctgcg
     2401 cgcttcgggt aagttaccgg ttctgaacag tgtgcttatt gcgctgtaca gttaaccttg
     2461 caatttaagc gcgctgtttt tgccctcgac gactttgcgc cccggctgga aacgaattta
     2521 aacagcccct ttagatgttg cttgaagtta tcgcaggctc cgcacgttaa aaaacaacag
     2581 ctagtttaac gaagctgttt aacagggcta aacgtgaaaa ctctccccgc aaccgcattc
     2641 gttttttacg ttcgggttat tgaatttaaa tccctcgttt aaaccctctt tagcaaaatc
     2701 caactcggta ccatctaaat aggccaaact tttgggatct actaccaggg aaaaaccttc
     2761 ctgcggaaaa atcacatcat cttcggcaaa ggcatccaca aattccaaca cgtaggagag
     2821 gccagagcag ccggtggttt taacgcccag gcgaataccg gcacccttac cccgactggc
     2881 taaatgtttt tttacgtgat gcacggccgc ttgcgacata gtcaaagcca taatgcattt
     2941 ccccttacaa acaaattaaa cctattcacc gcgcttttta cgcaaatcat cgatagcggc
     3001 acgaatggca tcttcggcca atactgagca gtgaattttc accggcggca gagccaattc
     3061 ttcagctata tcaacatttt taatttgcgc cgcttcatcc agagttttgc cttttaccca
     3121 ttcggttaac aacgagctgg aggcaatggc cgaaccgcag ccgtaggttt taaatttggc
     3181 atcttcaata atgccctgct cgttcacctg aatttgcaac cgcataacat cgccacaggc
     3241 cggggcaccc accatgccag tgcctacgtt attggcctct gcatccaatt tgcccacatt
     3301 gcgtggattc tcataatggt caattacttt ttcactgtat gccataacct tacctcgcta
     3361 acgggccaac taatgggcag cccattccac cttatttaaa tcaatgccgt ctttgtacat
     3421 atcccacaga ggcgacagct cgcgcaattt attcaccgcg taatgaattt tttccaccgc
     3481 aaaatccacc tgctcaatcg tagtgccgcg gccaaaccca aagcgcagtg agctgtgggc
     3541 taattcatca gacaggccca atgcgcgcag tacataactc ggctctaagc ttgctgaggt
     3601 acaggcggaa ccggaggaaa ccgctaaatc tttcagcgac ataatcagtg attcaccttc
     3661 tacgaaggca aaactcacgt tcaaaatatg cggcacgctc tggcttaaag agccgttgat
     3721 atggacttgt tcaatatcgc taatgcccgc taaaaaccgc tcacgcaatg ttttggcgtg
     3781 ctgataatcg gcctccaaac gggctttagc caccgcagcg gccgccccta aaccgacaat
     3841 ttggtgggtg ggcaaggtgc ccgaacgcat accgcgctcg tggccgccac cgtgtatttg
     3901 cgcttcgatg cgtacgcggg gtttgcggcg cacgtacaaa gcgccaatgc ctttagggcc
     3961 atacattttg tgagcggaaa tagacaacaa atccaccggc aactgctcca tatccacggg
     4021 cagtttgccc aggctctggg cgccgtccac atggaaatag acatggcgct cacggcaaac
     4081 cgcacctatg gcggctatgt cgttgatcac gccaatctcg ttatttacat gcatcaagga
     4141 aactaaggtg gtgtccgggc gaatggcggc ggcaaccgcc tccggggtga tgatgccttc
     4201 actggtcggc tccagatagg tcacttcaaa cccttcccgc tctaattggc ggcaggtatc
     4261 cagcaccgct ttgtgttcaa ttttggaggt gataaggtgt ttgccgcgct tgctgttaaa
     4321 cagagcaacc cctttaatgg ccaggttgtc cgattcggtg gcaccgctgg tccacacaat
     4381 ttcacgagga tcggcattaa tgagttgtgc cagctgttcg cgggcatgct caaccgcttc
     4441 ctcggccttc caaccaaaca catgggagcg ggaggccggg ttgccgaagt taccctcgcg
     4501 ggttaaacac tgcatcatgg cttcggcaac gctggggtct accggcgtgg tggcggaata
     4561 atcaaaataa attggggttt gcatagcggt tcccactctt caagggctgt taactcatag
     4621 cccataccta gttaattcaa acgtcgctca gtgccgctta tacgagcgga gagagcgtga
     4681 ttctctcttc agtagcattt tccagctcat cctgacgcag gcttacccgc cgcacatcct
     4741 gccgagccac caagcttgcc aggctgatgc cacttaaaaa ctgatggatc tgatcggaca
     4801 gatcactcca taaatagtgg gttaaacaca cctcaccttt ttggcaattg ccctggccgc
     4861 cacaatgggt ggcatccacg gattcattca ccgcgtcgat
//
LOCUS       DAOQGS010000304         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9055_length_4883_cov_4.686620, whole genome shotgun sequence.
ACCESSION   DAOQGS010000304 DAOQGS010000000
VERSION     DAOQGS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9055_length_4883_cov_4.686620"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKZ52_11615"
     CDS             complement(<1..673)
                     /locus_tag="PKZ52_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60463.1"
                     /translation="MKLVIFLTLALLSLNVLGRPTIQDFTRERDLRTTVLSPNGRYIA
                     SIVNKDQMRILVITDLQAPNNPIIGRLNQKITRPKFVSWANDERLLVTIDVPYDTASV
                     ERDAQKKKDFNIDDYFMFSRIVSFDLHAQNPVTLLNDDKNLRWNINLSSIHHFLPNDP
                     NNILMNVIKDDVICLSKVNVYNGTSETVAKGTRFTVAFISDYDGNTRYRYDYKPIAKE
                     VLIYES"
     gene            complement(900..2252)
                     /locus_tag="PKZ52_11620"
     CDS             complement(900..2252)
                     /locus_tag="PKZ52_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNG60464.1"
                     /translation="MRLINTRRTFLKSMGLGASCAATFSSGILPLVANAAATGSDYKA
                     LVCINLNGGNDAFNTLVPSSDALYNQYSKIRQELALPKDKLNLLKNSSGQSAGFGLHP
                     QLNGLAELYNTGKLAAVANLGNLIEPVEKSRYKQNNTQLPANLFAHNDQAVFAQTLNN
                     GASDTGWAGRIAEALGEININQQLAMNITLSGANPWQRGQEHLPFGLLKSGIANIDAL
                     AQSDAAARARVYLKLLANKRSNPLQQFYTDVVSNSLVMSKYVGDILNATRIPAPLNTA
                     EGFSQNFSIIAKMIAAHQDFNIQRQIFFVELGSFDTHANQLKGQEGLLSELNKALTDF
                     YSTLTAMGYADKVVSFTLSEFGRTLSRNGTDGTDHGWGSHHFVMGDAVKGQQIIGTLP
                     SLELGSDDDIGEGRIIPTISFEQYAATLSKWFGVSDEDLVNIFPNLKNFNNKNLGFLS
                     "
     gene            complement(2257..4149)
                     /locus_tag="PKZ52_11625"
     CDS             complement(2257..4149)
                     /locus_tag="PKZ52_11625"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNG60465.1"
                     /translation="MLHLRFGIALCALIACTTGCKNSHKNSDSSAASSSSSSSSSSSS
                     SSGVSIVLKPDLAPDPQLPTRGSPNWNKAAEAARFLSRASFGPTAKDIDYIIQNGQAA
                     WLEQQLALPATPQLKLFTKRLQDLGFTPYLPWMINFADLSAEEYHFERVVFQHHIWME
                     TAIWGQDQLRARVAQALSQILVVSQFGAAEFHRETGFANYTDILMEGAFGNYAELLSK
                     VTLNPIMGIYLNTLNNPKADEAKYTRPDENYAREVLQLFSIGLSELNTDGSLKLDAKG
                     KPIPTYNQETIKEFARVFTGWSFATYSEFAENGPWTLYPAPIANDMELKAFPEFHDTG
                     SKTLLNGAVIPAGQTPRQDINAAIANIMAHPNVGPFISKKLIQFLITSNPSPDYVKRV
                     ADVFNDNGKGVKGDMRAVVKAIYLDAEFYQPIDAKSSYGKLKETPLAIAGIWRAFKAQ
                     GVPIRSPLNNSVITIAHHHYNSEEQEMLHAPSVFNFYSSDYSPPGALTRQAILAPEAQ
                     IFGGLLAVYQNNLLANLIFQRDISDKNIYTGAAAGEVKGIIPYGTYYDPIVKALLNLT
                     EERALADKPAELIDRINLLLTQGQLSAEDSALIAEHIKTIADPLARIYEAIYLIAISP
                     EYAVQG"
BASE COUNT         1187 a         1045 c         1287 g         1364 t
ORIGIN      
        1 gtgactcata aataagtact tctttggcaa tgggtttata gtcgtaccgg tagcgtgtat
       61 tgccgtcgta gtcgctaata aaggcgacgg taaatcgagt tcctttggcg acagtttcgc
      121 ttgtgccatt ataaacattc actttactca ggcatatgac gtcatctttg ataacgttca
      181 tgagaatgtt attggggtca ttgggtaaaa aatgatgaat gctagaaaga ttgatattcc
      241 agcgtagatt tttgtcgtca ttcaataaag tgacaggatt ttgggcgtgc agatcgaagg
      301 atacaatgcg cgaaaacata aaataatcgt ctatgttgaa atcctttttc ttctgggcat
      361 cacgctcgac ggacgcggtg tcatagggga catctatagt cactaataag cgctcgtcat
      421 tcgcccagga tacaaatttt ggtcgggtaa ttttttgatt aagtcggcca attattggat
      481 tgttgggcgc ttgtaaatcc gtaatcacta aaatacgcat ctggtctttg ttgactatgc
      541 ttgcaatgta gcgcccatta ggtgatagca ctgtggtgcg caaatctcgt tcccgagtaa
      601 aatcttgaat ggtgggtcgc cccaatacgt ttagagatag aagtgctagt gtcaaaaaaa
      661 tgactaattt catcgggttt ccctgtggag agctaataac taataaacta ataaatagaa
      721 gggttcgata agtttggtag ctcacgcttt gtgagctaca ggcacgataa ggtaagttgt
      781 aatgtgcgtc aatttactca ggcgatctgt atgtttttta cacccccctg cgtctttgga
      841 gttatccgtt ttttagctaa atttaccaga tcattttcgc ctaggtattc aacaggcgcc
      901 tagcttaaaa atcccagatt tttattatta aagtttttaa gattgggaaa aatattcacc
      961 agatcctcat cgctaacacc aaaccatttg cttaaggtgg ccgcatattg ctcgaagcta
     1021 atggtgggaa taatgcgccc ttcgcctata tcgtcatcgc tgcccaattc taagctcggt
     1081 aaagtgccga taatctgctg gcccttcacc gcgtctccca tgacaaaatg atggctgccc
     1141 cagccgtggt cggtgccgtc agtgccgttg cgggaaaggg tgcggccaaa ttcggagagg
     1201 gtaaagctca ctaccttatc tgcgtagccc atagcggtaa gtgtgctgta gaaatccgtc
     1261 agggctttgt tcagttcgct taataaaccc tcctggcctt tgagctggtt ggcgtgggtg
     1321 tcaaaggagc ccagctcgac aaaaaatatt tgccgctgaa tattaaaatc ctgatgggcg
     1381 gcgatcattt tagcgatgat agaaaaattc tggctaaacc cttcagcagt atttaagggg
     1441 gcagggatgc gggtggcatt taatatatcg cccacatatt tagacatgac cagggaattg
     1501 ctcaccacat cggtataaaa ctgctgcagt gggttgctgc gtttgttggc caataatttt
     1561 aagtacaccc gcgcacgtgc cgcagcgtcg ctctgcgcta gcgcatcaat attggcaata
     1621 cctgatttta ataggccaaa aggcaggtgc tcctggccgc gctgccaagg gttggcgcct
     1681 gagagggtaa tattcatggc caattgttgg ttgatattaa tctcacctaa ggcctcggca
     1741 atacgcccag cccagcctgt gtcagaggct ccattattta aggtttgggc aaataccgcc
     1801 tggtcgttgt gggcaaataa attggccggt aattgagtgt tgttttgttt gtagcgggat
     1861 ttttccaccg gttcgattaa attgcccagg ttggcaacgg cggcgagttt gccggtgttg
     1921 tacagctcgg caaggccgtt aagttgcggg tgtaagccga aaccagcgct ttggccactg
     1981 ctgtttttta gcaggtttaa tttatctttt ggcagggcta gctcctgccg aattttgctg
     2041 tattggttat agagtgcatc gctgctgggc acgagggtat taaaggcgtc attgccaccg
     2101 tttaggttga tacacaccag tgctttgtaa tcgctgccgg ttgcggcggc attggccact
     2161 aggggcaaaa taccgctgct aaacgtggcg gcacagcttg cgcctaggcc catggatttt
     2221 aaaaaggttc tacgggtatt aatcaggcgc atggatttat ccttgtacgg cgtattcagg
     2281 ggaaatggca attaaataaa tagcctcata aatgcgcgca agcggatcgg caatagtttt
     2341 tatatgctcg gcaattaacg ccgaatcctc tgcactcaac tgcccctggg tgagcagcag
     2401 attaatgcgg tcaatgagtt ctgccggttt atcggctaag gcccgctctt cagttaaatt
     2461 cagcagggct tttactatgg ggtcataata agtgccgtag ggaataatac ccttgacttc
     2521 accagcggct gcaccggtat agatattttt atcgctaata tcgcgctgga aaataagatt
     2581 ggccaatagg ttgttctggt ataccgcaag caggccgccg aagatttgtg cctccggtgc
     2641 gagaatagcc tgccgtgtca gggcgccggg cgggctgtaa tcgctggagt aaaaattaaa
     2701 tactgacggc gcgtgcagca tttcttgttc ttcagagttg taatggtggt gggcgatggt
     2761 gatgacggaa ttattaaggg ggctgcgaat gggcacgccc tgggctttaa aggcccgcca
     2821 aatacccgcg atggccagcg gtgtctcttt taatttgcca tagctacttt tggcatcaat
     2881 cggttggtaa aactccgcat ccaggtaaat ggcttttacc actgcgcgca tatcaccttt
     2941 tacgcctttg ccgttatcgt taaatacatc ggcaacgcgt tttacatagt cggggcttgg
     3001 gttgctggta attaaaaatt gaataagttt tttgctgata aacggcccca cattggggtg
     3061 ggccataata ttggctatgg cggcattaat atcctgccta ggtgtttgcc cggcgggaat
     3121 aacagcgcca tttaacagtg ttttgctgcc ggtatcgtga aattctggaa aggctttgag
     3181 ttccatgtcg ttggcgatgg gggcagggta gagtgtccag gggccatttt cggcaaattc
     3241 tgagtaggtg gcaaagctcc agccggtaaa tacccgcgca aattctttaa tggtttcctg
     3301 attgtaggtg gggatgggtt tgcccttggc atcgagtttt aaactgccgt cggtattgag
     3361 ttcgctcagg cctatgctga agagttgtaa tacttcgcgg gcgtagtttt catccgggcg
     3421 ggtatattta gcttcatcgg ctttggggtt atttagggta tttaaataaa tgcccattat
     3481 ggggttgagg gttactttgc tgagtaactc ggcataattg ccaaaggcgc cctccatgag
     3541 aatatccgtg taattagcaa agccggtttc ccggtgaaat tccgcagcgc caaattgaga
     3601 caccaccagt atttgactta acgcctgggc aacccgcgcg cgcaattgat cttggcccca
     3661 aatagcggtt tccatccaaa tatgatgttg aaataccacc cgttcaaagt gatattcctc
     3721 agcgcttaaa tcggcgaaat taatcatcca gggtaggtaa ggggtaaagc ctaaatcctg
     3781 taagcgtttg gtaaacagtt ttaactgcgg tgtggcgggc agagctaatt gttgctcgag
     3841 ccaagcggct tggccgtttt gaatgatgta atcaatatct ttagccgtgg ggccaaagga
     3901 tgcccgcgat aaaaaccgcg cggcctcggc ggctttattc caattggggg agccccgcgt
     3961 tggcaattgc ggatcggggg cgagatccgg ttttaacaca atgctcacac cggaggatga
     4021 cgaggatgaa gacgagctgg aagatgacga actggctgcg ctgctgtcgc tatttttatg
     4081 gctatttttg cagccggtgg tgcaggctat taatgcgcat aatgcgatac caaaccttag
     4141 gtggagcatg ggaggtcctt agcgggggcg atgatgaggc tttaaatgga cgctgcggtg
     4201 aacaggtttt acagcctaat gttgtaaggg ccggattact atcaacccgg cggccaaata
     4261 gatcgtcctg atctatttgg ccttcgcagc gcaaaaactg gcgaaaatac gtggattttc
     4321 ttgtttttgc gcagctcgcg gcggtttgca ccgccgagtg cggcatcctt gccgccatta
     4381 ttaacccggc actaaatgcc gccgggtcaa tataaagcgc cgcgctaaaa aaatcccgct
     4441 cagccagtgg tgcggcacgg ctaaatgttc tgattgcgaa cctattcaaa actcgttgtt
     4501 tgtttgccgg gcggcgctgg tgagattaat tttggcccta cctatttccg cacactgtaa
     4561 tttttattta cgcgcctttt tgtcgcttgc agtgtgtttg gggggcaacc tgcaccaggg
     4621 gtaggtgtgt tttggatcct gcgactgcgc gcaggatgac aggttaaaaa caccaggatg
     4681 atttatttaa aaatgcggag gatgacgcat ttaaaattgc tgtggatgac gtgttgcaaa
     4741 attcgctgtg tagaacgctc ataactaact ggggtggttg tgcctgatgg ttcaaaaaaa
     4801 atgctacagg tcaataaatt caattcaagt tactagctgt gcggaagcaa tgaatacaca
     4861 tcatttcaga ccgtcaaaaa gag
//
LOCUS       DAOQGS010000305         4864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9111_length_4864_cov_4.443335, whole genome shotgun sequence.
ACCESSION   DAOQGS010000305 DAOQGS010000000
VERSION     DAOQGS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9111_length_4864_cov_4.443335"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="urtC"
                     /locus_tag="PKZ52_11630"
     CDS             <1..720
                     /gene="urtC"
                     /locus_tag="PKZ52_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNG60466.1"
                     /translation="PGVLAFVFGWLAFRSRVSGVYLSIITQALTYALMLAFFRNDMGF
                     GGNNGLTDFKEIIGFDLQATSTRIVLFILSVAALILAYCGARYITASKLGRVLVSVRD
                     AESRTRFLGYRVEHYKLMVFVVSALLAGIAGALCVPQVGIINPSEFNPLNSIEIVIWV
                     AIGGRGTLYGAVLGALLVNYGKTYFTSGVFAEYWLFILGALFVVTTIYLPRGIVGLKE
                     QWASGKLAALFKNGASAKVAS"
     gene            717..1469
                     /gene="urtD"
                     /locus_tag="PKZ52_11635"
     CDS             717..1469
                     /gene="urtD"
                     /locus_tag="PKZ52_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNG60467.1"
                     /translation="MSDTAVKSILYLNGVNKTFDGFKAINNLCLDIRPGELRAIIGPN
                     GAGKSTMMDIITGKTKPDTGEVIFRDQIDLTRHDEADIANLGIGRKFQKPTVIGSLTV
                     WENLELALAGARGVWQTLFFKLSAEKHARLESVLAQVGLAARRDELSFNLSHGQKQWL
                     EIGMLLVQDPQLLLVDEPAAGMTDYETLETAKLLKGISQHHSVVVVEHDMDFIRALDT
                     KVTVLHEGSVLAEGRLEHLQQDQRVIDVYLGR"
     gene            1473..2168
                     /gene="urtE"
                     /locus_tag="PKZ52_11640"
     CDS             1473..2168
                     /gene="urtE"
                     /locus_tag="PKZ52_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNG60468.1"
                     /translation="MLEAKQINLYYGASQALQQVNVIAKKGEITCILGRNGVGKTSIA
                     RAITGRHPISAGEIIWQGESIHGMSAADRAKRGISYVPQGREIFSQLTVWENLKTGFA
                     VLPRKERKVSDEIFDLFPVLRTMLQRRGGDLSGGQQQQLAIARALVMKPGLLILDEPT
                     EGIQPSIIKDIHNVISILRRQGEMAVLLVEQYFEFAKTLADAYVVMDRGVAVVSGRMA
                     EIDEAKVKQHLMV"
     gene            complement(2750..3673)
                     /locus_tag="PKZ52_11645"
     CDS             complement(2750..3673)
                     /locus_tag="PKZ52_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNG60469.1"
                     /translation="MRKLFKKLGVCSSCLLCACSLIRPPPAQLLRESYTSQLDQSTKQ
                     FYVYLPKGYNRKKAWPVLMFLHGDGERGNGREELGYVLKHGPLYEAWIQKKDLPFIII
                     SPQLPMFERDKLPEMAYIRSRTLAEIPQREASTPARTYMPPAFPMARAPELSDWNGVA
                     PLLPDGWERVEADLLAILTQVQTRYATDNSRIYLSGLSYGGFGTWYMASRHPQLFAAI
                     APVVGWGHASFMQPIAQAHLPVWAFAAGRDEAVPISHFYAGMRALEAFGSSEVRFTVH
                     EDMAHDAWKRIYAGDDLYNWLLAHRHEQPKP"
     gene            3888..4211
                     /gene="trxA"
                     /locus_tag="PKZ52_11650"
     CDS             3888..4211
                     /gene="trxA"
                     /locus_tag="PKZ52_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNG60470.1"
                     /translation="MSGIVTVSDASFETEVVNASGPVLVDFWAPWCGPCKMIGPILEK
                     LAGVYGDKIKICKMDVDANKETSAKLNIRGIPTLIIYKGGKAESTKVGALSETQLKAF
                     IDAAL"
     gene            4470..>4864
                     /locus_tag="PKZ52_11655"
     CDS             4470..>4864
                     /locus_tag="PKZ52_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNG60471.1"
                     /translation="MNLTDLKKKPIEELIEMAAAVGMESLARSRKQDVIFNLLKRHAK
                     SGEDIYGDGVLEILQDGFGFLRSAGSSYLAGPDDIYVSPSQIRRFNLRTGDAISGKIR
                     PPKEGERYFALLKVNDINTDRPESSRNKIL"
BASE COUNT         1137 a         1079 c         1334 g         1314 t
ORIGIN      
        1 cctggagtgc tggcatttgt atttggttgg ctggcgtttc gctcgcgggt gagtggggtt
       61 tatttatcca ttattaccca ggcccttacc tatgcgttga tgctggcgtt tttccgcaat
      121 gatatggggt ttggcggcaa taatggtttg acagatttta aagaaatcat tggttttgat
      181 ttgcaagcca cttcaacgcg tattgtgttg tttattttaa gtgtagcggc gttgatattg
      241 gcctattgcg gcgcccgtta catcactgcg agtaaattgg gccgggtgtt ggtttcggtg
      301 cgggatgctg aaagccgcac gcggttttta ggctataggg ttgagcacta taaattaatg
      361 gtgtttgtgg tatcggcact gctggccggt atagctggcg ccctttgtgt gccgcaggtg
      421 ggtattatta accccagtga gtttaaccct cttaactcca ttgaaattgt tatttgggtg
      481 gccataggtg ggcgcggtac tttgtatggc gctgtgctgg gtgcgctgct ggtgaattac
      541 ggtaaaacct attttaccag tggcgtattt gccgaatact ggttgtttat tttgggcgcc
      601 ttgtttgtgg taaccactat ttatttgccg cgaggtatag tcggtttaaa agagcagtgg
      661 gccagtggca aattggctgc tttatttaaa aatggcgcga gcgcgaaggt ggcatcatga
      721 gcgacacagc agttaaaagt atcctctact taaatggtgt caataaaacc tttgatggtt
      781 ttaaagcgat taataatctg tgcctggata ttcgccccgg cgagctgcgc gcgattattg
      841 gccctaatgg tgcgggcaaa agcaccatga tggatattat tacgggtaaa actaaacccg
      901 acacgggcga ggttattttt cgcgatcaaa ttgatttaac tcgccacgat gaagcggata
      961 tagcaaacct gggtattggc cgtaaattcc agaagcccac ggtcattggc agccttacgg
     1021 tgtgggaaaa tttagagttg gctttggcag gtgcccgcgg cgtctggcag acgctgtttt
     1081 ttaaactgag cgccgaaaaa cacgctcgct tagaatcggt gttggcgcaa gttggccttg
     1141 cagcgcggcg ggatgagttg tcttttaatc tctcccacgg tcaaaagcag tggttggaaa
     1201 tcggtatgtt actcgtgcag gacccgcaat tattgttggt ggatgagcca gcggcgggta
     1261 tgacggatta tgaaacctta gaaaccgcta aattacttaa aggtatcagc cagcatcact
     1321 ctgtggtggt ggtggagcac gatatggatt ttatccgcgc tttggatact aaggttacgg
     1381 ttttacacga agggtctgtg ctggcggaag ggcggttaga gcatctgcaa caggatcaac
     1441 gagttattga tgtgtatttg gggcgctaaa taatgttaga agccaagcaa attaatttgt
     1501 actacggcgc cagccaagcc ctacagcagg taaatgtgat cgctaaaaaa ggtgagatca
     1561 cctgtatttt agggcgcaat ggcgtgggta aaaccagtat tgcgcgggcc attaccggtc
     1621 gccatccgat ttcggcaggg gagattattt ggcaggggga gtctattcac ggcatgtccg
     1681 ctgcggatcg cgccaagcgg gggatttctt atgtgcccca ggggcgcgag attttttcgc
     1741 aattaacggt gtgggaaaat ttaaaaactg ggtttgcggt gttgccgcgc aaggagcgca
     1801 aagtgagcga cgaaattttt gatttattcc ctgtgttgcg caccatgttg cagcggcgcg
     1861 gtggggattt atccggtggg cagcaacagc agctcgccat cgctcgcgcc ctggtgatga
     1921 agcctggcct gttaattttg gatgaaccca cagaaggtat tcagccgtcg attattaagg
     1981 atattcacaa tgtgattagc attttgcgca ggcagggtga aatggcggtt ttattggtgg
     2041 agcaatattt tgaatttgcc aaaaccctgg cggatgctta tgtggtgatg gatcgcggtg
     2101 tggcggtggt gagcggccgc atggcggaga ttgatgaggc caaggtaaaa cagcatttaa
     2161 tggtttagtg ctttgtaacc attcagcaca tttttactac ccagtctgca gcagtgaata
     2221 cacattattt caaacgcgcc gtgaatacgt gcgtgcagac agtcagaaag gtcccccctt
     2281 ttgactgtct gcacaaatct gtagtcacca ctgcaccacc gcgcatcgat tatgctgcat
     2341 ggctaaatct tcgagagctt gctgcccgcg cgcaagagag taataaaggg agtaataaag
     2401 agagtaataa agtgttctga gtagttttgt tttttctatt ggtcgactgc agcgagtttt
     2461 aactgggatt gtggtgttgg gattattcgg ccacctattt attggctttt ttattagatg
     2521 ttgttttccg gggttaacct aaaaatctca gttaaacctc acagagaacg cctcgcccgc
     2581 ttaacgtcta agcactgcga gggctcggcg ggatttatgt cggaagtcgc tgcgctcttg
     2641 ttacttacgc tagaacgggc ttgctcctta cttttaatga catttggggg aaggcgcaaa
     2701 ataagggtaa gtagcaaaat cctcgtcctt tactcgcagg aataaaaatt tagggtttcg
     2761 gctgttcatg gcgatgggca agcaaccagt tataaagatc atcaccggca taaatgcgtt
     2821 tccaggcgtc gtgggccata tcttcgtgga cggtaaaacg cacctctgag ctgccaaagg
     2881 cttccagtgc gcgcatgccc gcataaaaat ggctaatcgg caccgcttcg tcgcggcctg
     2941 cggcaaaggc ccagacgggt aggtgggcct gggcaatggg ctgcataaaa cttgcgtggc
     3001 cccagcccac caccggtgct atagcggcga acagttgtgg gtggcggctg gccatatacc
     3061 aggtgccaaa accgccgtag ctgagcccgc ttaaatagat gcggctgtta tcggtggcgt
     3121 agcgggtttg cacctgggtg agtatcgcca ataaatccgc ctccacccgc tcccagccgt
     3181 ccggcaataa gggggcaaca ccgttccaat cgctcagttc cggtgcccgc gccatgggaa
     3241 aggccggtgg catataggtg cgggcaggtg ttgatgcttc gcgctgggga atttccgcta
     3301 gggtgcggct gcggatatag gccatctcgg gcagcttgtc gcgctcaaac atgggcaatt
     3361 gcggtgaaat aataataaac ggcaagtctt tcttttgaat ccaggcctcg tacagggggc
     3421 cgtgtttcaa cacatagccc agttcctcgc gcccgttgcc gcgctcgcca tcgccgtgca
     3481 gaaacatcag caccggccaa gcttttttcc ggttgtagcc tttagggagg tagacgtaaa
     3541 actgtttggt gctttgatcc agctgtgagg tgtatgattc gcgcagcaat tgcgcaggag
     3601 gcgggcgaat taaactgcag gcgcagagca ggcagctgct gcaaacccct aattttttaa
     3661 acaatttacg cataaaaaat acccgcaacg attggaagct ggcaagttta ggtgtttggg
     3721 ggcggctttc ggaagaaaat tccctgctcg ggcttgagtt ttgttatttg cgcctcaaca
     3781 ttcgccaaat gctgtctaga cttagaaggg ccgaggggtt gtcacagggg caggcactcc
     3841 ttattattgc gccgtttatt aaatgcccaa tgcggagata acaggttatg agcggaattg
     3901 ttaccgtcag tgacgcaagt tttgaaacag aagtagtaaa tgcctcaggt ccagttctgg
     3961 ttgatttttg ggcgccctgg tgtggtcctt gtaagatgat cgggccaatt ttagaaaaat
     4021 tagccggtgt atatggcgat aaaattaaga tctgcaaaat ggatgtggac gctaacaaag
     4081 agacttcagc caagttgaat atccgcggta tccccacttt gatcatctat aaaggcggca
     4141 aggctgagag caccaaagtg ggcgctttgt ctgagaccca attaaaggcc ttcatagacg
     4201 cggcacttta atcgcattta taagccacag cctgctgtgg cttgcctttt ttctttgcac
     4261 aacttttgag aatcgctata ctccacttaa ttgctgaaag gcttgccgaa ccctaatcct
     4321 ccggcgccgt gtttgccatc cccgattggc acaccctcag gtcaagtgct ctcttctttc
     4381 ctcctcgctt cttttgcttg accctaggca catcaacaat tttctaagcg cttgcgttta
     4441 tcaactcctc aataagacaa ataacactca tgaatctaac cgaccttaag aaaaaaccca
     4501 tcgaagagct tatcgaaatg gccgctgctg tcggcatgga gagtttggct cgctcccgta
     4561 aacaggatgt tatttttaat ttattaaagc gccacgcaaa aagcggcgag gatatttacg
     4621 gtgatggtgt tttagaaatt cttcaggacg gttttggttt tttgcgctcg gcaggttctt
     4681 cgtacctggc ggggcccgat gatatttatg tatctcccag ccaaattcgc cgctttaacc
     4741 tgcgcacggg cgatgccatc tccggcaaaa ttcgcccccc caaagagggt gagcgctatt
     4801 ttgccttact taaagtcaac gatattaata ccgaccgacc cgagagttcg cgcaataaaa
     4861 ttct
//
LOCUS       DAOQGS010000306         4836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9191_length_4836_cov_3.972809, whole genome shotgun sequence.
ACCESSION   DAOQGS010000306 DAOQGS010000000
VERSION     DAOQGS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9191_length_4836_cov_3.972809"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKZ52_11660"
     CDS             <1..1359
                     /locus_tag="PKZ52_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNG60472.1"
                     /translation="THEVGHNMGLGHSYIQNSPGGVFSWGRGHGVMGLFSTVMAYPQS
                     YGTTNQLPIFSNPSLKTCAGSACGVDRARTDGADAAGSLNTLATQISNFMPAIVPLAP
                     ASSSSSSSSSSSSSGKISSSSSSSSSSSSSSSSGQTAVACNRSVSADNLIKNGEFTLL
                     DGWVSGLGASSLALKEVVNGTCTDKVLWVNNRKSYLGDSFYTLGTALVAGTKYRLTAR
                     TSIRNSARDNVRVAMVVASSSAKQLYELPMVSVTPNEFTSYSAEFVAPVSGINGVLFF
                     GPQTGVDFLLDGVELTKVVTPAAPTNTVLINEGFEASSNGWSAVFGGVASSVNTNPAE
                     GKYCLRISQRTSVNSGAAVQLGAQLLAGKNYALSAKLRVGSTATAAANVQIWLAFNDS
                     RGAQKISLGAGSIPGNVWASLNTSFNVYAVGAMTNAQIMFVGPAAGQDLFVDAVKLNS
                     VN"
     gene            complement(1543..2082)
                     /locus_tag="PKZ52_11665"
     CDS             complement(1543..2082)
                     /locus_tag="PKZ52_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG60473.1"
                     /translation="MCALELPPSKIANALWLVPTTDLHQAFLQRVFTQSRKALWQASG
                     LDAPWIDKLLEDQYNLQQKNYGAQENGRYSYVIAAKGQWVGRLLVRADASTLHIMDIA
                     LLPEYQNQGIGQHVLTYLQQQASNRGQLLTLNTDATNRAYHWYLRCGFTVTQQVGIDC
                     AMAWRAPTSADVSHYSLVQ"
     gene            2927..3469
                     /locus_tag="PKZ52_11670"
     CDS             2927..3469
                     /locus_tag="PKZ52_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNG60474.1"
                     /translation="MSDAYLGEIRIFAGDFAPVNWMLCQGQILSIASNNALFAVIGTF
                     YGGNGVTTFALPDLRARTPVGTGGGGPFDAVTIGEASGQQSVTLTQQQLPQHTHLVSV
                     ATEPANPVSAPTSTNNILGASSAGPGTANIWSDALGTAVTLAPNTVGLAGANLPFSIQ
                     NPYLGLSFIICTAGIFPSRQ"
     gene            3659..>4836
                     /locus_tag="PKZ52_11675"
     CDS             3659..>4836
                     /locus_tag="PKZ52_11675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG60475.1"
                     /translation="MDWTAGYVSDVEYTAGFYSEQAPGLLNFACILNGVEPVDTSKGF
                     NYCELGFGRGLTVNVLAAANPQGRFYAADFNPSHVAGARQLAASAELANLFLLESSFE
                     QMALGEVEGLPQFDFITLHGIYTWVTAENRAHIVQFISRYLKPGGIVYLSYNAMPGWA
                     CAQPLQKILVEHAALNPNRSDVQIHHAAQFVDKLQQLNAGFITANPGLKPRLATLNNA
                     DRHYLVHEYMHRHWQPMYHIDVANDLAEAKLDYVGSADLPLAYRQMYLTAEQFALLES
                     ISDRRMRETVHDYLLNIGFRKDIFVRGARRISQLRQSEYLAQLGLVALLPRDDCQMAF
                     KLNVGEMHGKPETYAPLFDRLAQGPQSFAQLNALFGGGGRMSPSLIEAASLLIATGQA
                     A"
BASE COUNT         1151 a         1088 c         1258 g         1339 t
ORIGIN      
        1 acccacgagg tgggccacaa tatggggctt ggtcattctt atattcaaaa ttcacctggc
       61 ggagtgttta gctgggggcg cggccacggt gtgatgggtt tatttagtac ggtaatggcc
      121 tacccacagt cctacggcac tacgaatcaa ttgccgattt tttccaaccc aagccttaaa
      181 acctgtgctg gtagtgcctg cggagtggat cgtgcgcgca ccgatggcgc cgatgccgct
      241 ggcagtttaa ataccctagc cacacaaatt agtaacttta tgcccgcaat tgtgccgctg
      301 gcacccgcta gcagttcatc gtctagttca tcgtcctcaa gtagcagcgg taaaatttct
      361 tcaagctcat ccagttcaag tagctcaagc agttcaagct cttccgggca aacagcggta
      421 gcctgtaatc gcagcgtgtc ggcagataat ttaattaaaa atggggaatt tacccttttg
      481 gatggttggg taagtggatt aggcgcgagc agcttagcgc ttaaagaggt tgttaatggc
      541 acttgcaccg ataaggtgtt gtgggtgaat aatcgtaaat cttatttagg tgacagcttt
      601 tacactctag gtacagcttt ggtggcgggc actaaatacc gcctcaccgc ccgcaccagt
      661 attcgcaata gcgcacggga taatgtgcgg gtagcaatgg ttgtggccag cagtagcgca
      721 aaacaattgt acgaattgcc catggtgtcg gtaaccccta atgaatttac cagctacagc
      781 gcagaatttg tggcgcctgt cagtggtatt aatggcgtgt tattttttgg cccacaaacc
      841 ggagttgact ttttgttaga tggagttgaa ttaaccaaag tggttacacc tgcggcgcct
      901 accaatacgg tgctgataaa tgaaggcttt gaagccagca gcaatggttg gtcggcggta
      961 tttggcggtg ttgccagcag tgtgaatacc aatcctgccg agggtaaata ttgtttgcgg
     1021 atcagccagc gcacgagtgt aaattctggc gccgccgttc aattgggcgc gcaattatta
     1081 gcaggtaaaa attacgccct gagtgccaaa ttacgggtgg gcagcacggc tacagcagcg
     1141 gctaatgtac aaatttggtt ggcatttaac gacagccgcg gtgcgcaaaa aattagcctt
     1201 ggtgcgggta gtattcctgg caatgtatgg gcgagcttaa atacaagctt taatgtatat
     1261 gccgtcggcg ctatgaccaa tgcacagatc atgtttgtgg ggcctgcggc tgggcaggat
     1321 ttatttgtgg atgccgtgaa gttaaattct gtgaattaat ttatttttaa attgatgcta
     1381 gtgcctgcaa cggctcggtg attcaccggg ccgtttttta ttttgggctg ctattgaacc
     1441 cggcacttgg taattaaggt ggggattaaa ctttaaacac tagcgcgcaa tagagctaac
     1501 aaaaaactag ctaaattcca tttagtgcag agcagtaagc ctttactgga ccaggctgta
     1561 atgacttaca tccgcgctag ttggggcgcg ccaagccatt gcgcagtcaa taccaacttg
     1621 ttgggtaact gtaaaaccgc agcgtaaata ccagtgatag gcccggttgg tggcatctgt
     1681 attgagggtg agcagttgcc ctcggttgct tgcctgctgt tgtaaatagg tgagcacgtg
     1741 ctggccgatg ccttggtttt gatattcagg caacagggca atatccatga tgtgcagagt
     1801 gctggcatct gcgcgcacca acaggcgtcc aacccattgc cctttggcgg ctatcacata
     1861 ggagtagcgg ccattttctt gggccccata gttcttttgc tgcaagttgt actgatcttc
     1921 cagcagctta tcaatccagg gggcgtcgag gccggatgcc tgccaaagcg ctttgcggct
     1981 ctgggtaaac acccgctgca gaaaggcttg atgcaggtct gtagtgggaa caagccagag
     2041 ggcattggca attttgctag ggggtaattc gagggcgcac ataggggtaa agccgttaaa
     2101 aagtctcggc taactgtagc tcggctgcgg cgttttgcac gctcttatgt gattatttca
     2161 cggctttttc tgcttgtaag gctacaggcc aggcgttgcg caaatagtca tttgacgatt
     2221 atgccatcct tcatcgaaga aaaattttat gctctgtctc ttctaggtta aggcgtttct
     2281 gacaagaaca cgtctgtgaa gggcttgtag ttgtcctgtg catatccggc cagcaagaaa
     2341 gcctcagcgg cgtgagcacc taactattag cctggcggcc ttttaaccag gttaataatg
     2401 ggcgacaagt atgccgcact gagggtgcca gtcgcttcac gctccgctaa cactcgaaat
     2461 tcagcgcact attacaaatc tcagccacaa gtctcagcca caagccccag cgcagcgaag
     2521 gcaaaataga tgaggactgt ctatttttgc tgccaataat acttcagccg cgcttagagt
     2581 gatggctgct caaacttcgt cagcagctta gccatagaac tttttggtgc ctgtgcttgc
     2641 atcctgctgg cattctgcta aacccttatg gccgcaggat ttttttacgc tgagcgcgtg
     2701 tttaacataa taataagttc agggtaagag tgtgcgtgtg gtctttctca tttaagttgc
     2761 cgactatggc agctgtggtc cgagtaataa ttaagttgta cgtaaaagtg taaataccgt
     2821 attaggcgca ggcagatacg cgttttactc cccgttactg tgcgtgtgat cgattggtgt
     2881 gttcgatgta catacacagg tttactccaa tttttgaggg tttgttatga gtgatgctta
     2941 tttaggtgaa attcgtattt ttgctggtga ttttgcgcca gttaattgga tgctctgtca
     3001 agggcaaata ttatcaattg cctctaataa cgcgttgttt gctgtgattg gtacttttta
     3061 tggcggtaat ggtgttacca cctttgcact accggatttg cgcgctcgta cgcccgtggg
     3121 tacaggtggc ggtgggccgt tcgacgctgt gactataggt gaagcgagtg gccagcagag
     3181 tgtcacactc acacaacaac aattacccca gcacacacac ctggtctctg tggcaacgga
     3241 gccagccaac ccggtttccg caccgacgtc aactaataat attttgggtg cttctagtgc
     3301 cggtccagga actgctaaca tctggtctga cgcgctaggt acggccgtca ctttggcgcc
     3361 taatactgtg gggctggccg gggccaattt gccgtttagc atacaaaacc cgtatttggg
     3421 cttgagtttt attatttgta cggcgggtat tttcccgtcc cgtcaataac aaatggtttg
     3481 agagccactg attaattagt gttttttaaa tcaccagggg caagatgcct gcgagtgcac
     3541 tacagggaag tatttgtgtg cccgcacact attcacgaat tcagacctaa tcagattatc
     3601 ctttaggttg cttaactttt ttgactcaaa ctaccaaaca ttaaataggg gtaaaattat
     3661 ggattggacc gcaggttatg tatctgatgt tgaatacact gcaggctttt acagcgagca
     3721 ggcgcctggc ctattaaatt ttgcctgtat tttaaatggc gtggagccag tggataccag
     3781 caaggggttt aattattgtg aactcgggtt tggccgtggt ttaacggtca atgttctagc
     3841 cgctgccaat ccccaggggc gcttttacgc cgctgatttt aacccctccc atgtggctgg
     3901 cgcgcggcag ttggccgcta gtgctgagct cgccaattta ttcttattag aaagtagttt
     3961 tgagcaaatg gccctgggtg aagtggaggg cctaccgcaa tttgatttta ttacgctaca
     4021 cggtatttat acctgggtaa cggccgaaaa ccgcgcccat atagtgcaat ttatcagccg
     4081 ctatttaaaa cctggcggga ttgtatacct aagttacaac gccatgcccg gctgggcctg
     4141 cgcgcagccg ttgcaaaaaa ttctggtgga gcacgcagcg ttaaatccca atcgctcgga
     4201 tgtgcaaatt caccatgctg cacaatttgt cgataaatta cagcaactca acgctggatt
     4261 tattacggct aatccaggtt taaaaccccg cttggcaacc ttaaataatg ccgatcgaca
     4321 ttatttagtg cacgaatata tgcatcgcca ttggcagcct atgtaccaca tagatgtggc
     4381 gaatgatttg gctgaagcta aattagatta tgttggctct gcggatttac ctttggctta
     4441 ccggcaaatg tatttaaccg cagagcagtt tgcgttattg gagtctattt ccgatcgccg
     4501 tatgcgcgaa acagtgcacg attatttatt gaatataggc tttcgcaaag atatttttgt
     4561 gcgcggtgcg cggcgtatat cgcaattacg tcaaagtgag tatttggcgc aattgggctt
     4621 ggtggcattg ttgccgaggg atgattgtca aatggcattt aaattaaacg tcggcgaaat
     4681 gcacggtaag cccgaaacct atgccccctt atttgatcgc ctggcccagg ggccacaatc
     4741 cttcgcacag ttgaacgcct tatttggcgg tggcgggcgc atgagcccat ctttaattga
     4801 ggcggcaagt ttattgattg ccactggcca agccgc
//
LOCUS       DAOQGS010000307         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9200_length_4833_cov_4.073252, whole genome shotgun sequence.
ACCESSION   DAOQGS010000307 DAOQGS010000000
VERSION     DAOQGS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9200_length_4833_cov_4.073252"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKZ52_11680"
     CDS             <1..300
                     /locus_tag="PKZ52_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60476.1"
                     /translation="DTQQNYESRLFINTAPVESGGRTIGYAYCGGSDVRTCYLSEGTF
                     YCSISYTGTVTCEDGRGSERIGDWLLNTHRFYLGLKICGAQGYGCTTKYREVTIY"
     gene            413..856
                     /locus_tag="PKZ52_11685"
     CDS             413..856
                     /locus_tag="PKZ52_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate pyruvate-lyase family protein"
                     /protein_id="HNG60477.1"
                     /translation="MTDGTVTKSLEALFNIPIKVIVQRQEPVAAADMAEGVVCDLGSE
                     RLLVRDVTLVRTDSHQLMAQAHSMIALDLLPAKLAAGLLAGELGIGELIRSQSLDTYR
                     QLVDLGISRREQALGIWRRYAICKQGQVLMQVEEWFPWTVYAAAV"
     gene            complement(1137..3170)
                     /locus_tag="PKZ52_11690"
     CDS             complement(1137..3170)
                     /locus_tag="PKZ52_11690"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HNG60478.1"
                     /translation="MHKPLFTSLFTCLLISACSGPDGTASGSSSSSSSSSSGSGRTSS
                     SSSSSSSSSSSSSTSSSSSSSSSSSSSGRTSSSSSSSSSGQTANNGKQMENLSRGLVA
                     VKTNNGVFLSWRSLGTDPANTAFNIYRDGNLITGQAISALTNYVDSAGTNNSQYSLRP
                     VIDGREQAAEQASVWASQSKIITLKRPSGGTGPDGVAYTYSPGDTSVGDLDGDGDYEL
                     VVKWDPSNAKDNSQSGNTGNVYIDAYTLEGQQLWRIDLGRNIRAGAHYTQFQVYDFDG
                     DGRAEVAMKTADGTRDGAGKVIGNADADYRNSAGYILSGPEFLTVFDGLTGKALATAD
                     YIPARGTVSSWGDSYGNRVDRFLAGTAYLDGKRPSMVFSRGYYTRAVVAAWDWRDGKL
                     TNRWVFDSNNDKSFEGQGAHSFSVADVDNDGKQEIIFGAATIDDNGKGLSNTKLCHGD
                     ALHVTDMDPNRPGLEAFMVHEDPSCYGNHGIEMHDPRTGQIIWSVQGGGDIGRGLAMD
                     VDPSQPGYEAWASTGNLYSATGRDLGAVKPAGVNFGIWWDGDLLREILDKNTISKWNP
                     SAKTTDRLLTASNASSINGTKAVPMLSADILGDWREEVILKGNDDSSLELFTTTIPSS
                     FKLYTLMHDAQYRVAIAWQNTGYNQPPHPSFFLGSGAVLPTTPADIYLSPRLK"
     gene            3530..4138
                     /locus_tag="PKZ52_11695"
     CDS             3530..4138
                     /locus_tag="PKZ52_11695"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNG60479.1"
                     /translation="MDKNRIEAFSDGVLAIIITIMVLELKVPHGIELSALIQLYPVFI
                     SYCLSFIYVGIYWNNHHHMFKAVHKVNGAALWANLVLLFWLSLLPFTTGWMGENHFAQ
                     WPVTVYGINLLFCALAYFVLQNVLIRHQGADGVLARAIGRDIKGKLSPVFYILGCTAA
                     WLSYPALGMVFFVAVALMWLVPDRRVETLVAKTPHVHEGESR"
     gene            complement(4221..4769)
                     /locus_tag="PKZ52_11700"
     CDS             complement(4221..4769)
                     /locus_tag="PKZ52_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNG60480.1"
                     /translation="MRRFKPGNFLPSIKLDSVNQGAISIPSPGVVTHLQFRRFAGCPM
                     CNLHLHQFKTRAQELIAANIQEVVVFHSTVESLKEFQADTPFAMIADPTKQLYRQFAV
                     QESIWAVAHPVALFTAIYAYIKGFRGAPSAEESVQGLPAEFLINEKGEIIAAHYGRHG
                     SDHWSFDDVLRKAKTMGTVSPT"
BASE COUNT         1125 a         1160 c         1281 g         1267 t
ORIGIN      
        1 gatactcagc agaactatga gtcccgttta tttatcaata cggcccctgt ggaaagtggt
       61 ggccgcacca ttggttatgc ctattgcggc ggcagtgatg tgcgaacctg ttacctctca
      121 gagggtacct tctattgcag tattagttat acgggtactg tcacctgtga agacggtcgg
      181 ggcagtgagc gcatagggga ttggttgctc aatactcacc gcttttattt aggattaaaa
      241 atttgcggcg cgcaaggata cggctgtaca accaaatacc gcgaggtaac tatttattaa
      301 tgctcaattt gccaaacagc taaaccaaaa tgggtttagt ttatattcac ttgagggccc
      361 ggaagggccc attccctatg cttcaattcc gccactgttg cggctgttat tagtgactga
      421 tggcaccgtc actaaatccc tcgaagccct atttaatatc cctataaaag taattgtgca
      481 gcgccaggaa ccggtggcgg ctgccgatat ggcggagggt gttgtctgcg atttaggttc
      541 tgagcggctg ctggtgcggg atgtcacact ggtgcgcaca gatagccatc aactaatggc
      601 acaggcgcac tcaatgatcg ccctggattt attaccggct aaattggcgg caggcctttt
      661 agccggtgag ttgggtatag gcgaactcat ccgcagccag agtttagata cctaccggca
      721 gctggtggat ttaggcatta gtcggcgaga gcaagctttg ggtatatggc ggcgctatgc
      781 catttgtaaa caaggacagg tgttgatgca ggtggaggag tggtttccat ggacggttta
      841 cgccgccgca gtttaataaa tttaattgat aataaaactg cgctggcggt ttctattaac
      901 ccagcgaata aatagatcgc cctgatctat ttatccttcg cagcgcgaaa cctacgaaaa
      961 tgcgcggatt ttctagtttt agcgcagctc gcggcggttt gcaccgccga gtgctgcatc
     1021 catgccgcca ttattaaccc ggcactaatt gtcgccggtt taataacact acccaaatta
     1081 ttgagtggtg gctgaataaa aatactgcgg caagcgcgaa actggccgca ggccatttat
     1141 ttaaggcgcg gcgacaggta aatatctgca ggtgttgttg gcaacacggc gccactgcct
     1201 aagaaaaagc tcgggtgcgg cggctggtta tagccggtat tttgccaggc aatcgccacg
     1261 cggtattggg cgtcgtgcat cagggtgtag agtttaaaac tgctgggaat tgtggtggta
     1321 aacaattcta agctgctgtc atcgttgccc tttaaaataa cttcttcccg ccaatcaccc
     1381 aaaatatccg ccgataacat aggcacggcc ttagtgccat taattgagct ggcattactg
     1441 gcggtgagta aacggtcggt ggtttttgcg cttgggttcc atttactgat ggtattttta
     1501 tcgagaattt cccgcagtaa atcgccatcc caccaaatac caaaattaac tccagccggt
     1561 ttaaccgcgc ctaaatctcg cccggtggcg ctgtataaat tgcctgtgct ggcccaggcc
     1621 tcgtaaccgg gttggctcgg gtccacgtcc atagctaagc cgcggccaat atcaccgccg
     1681 ccctgtacgc tccaaataat ttgcccggtg cgcgggtcgt gcatttcaat accatggttg
     1741 ccgtaacagc tggggtcttc atgcaccata aaggcttcca ggcccggccg gttagggtcc
     1801 atatcggtta cgtgcagggc atcgccgtgg cagagtttgg tattgctcag gcccttgcca
     1861 ttgtcgtcaa tggtggcggc gccgaagata atttcctgtt tgccatcatt atctacatcc
     1921 gccacagaaa acgagtgagc gccttggcct tcaaaactct tatcgttatt actatcaaat
     1981 acccagcggt tggtgagctt gccatcccgc caatcccaag cggccactac cgcgcgggtg
     2041 taatagccac gggagaacac catgctgggg cgcttaccgt ccaaataggc ggtacctgct
     2101 aagaagcgat ccacccgatt gccgtagctg tcgccccagc tactcaccgt gccgcgggcg
     2161 gggatatagt ccgccgtggc cagggcttta ccggttaagc cgtcaaagac ggttaaaaat
     2221 tctgggccgg ataaaatata acctgcgcta ttgcggtaat cggcatcggc attgccaatc
     2281 actttgcccg cgccatctct ggtgccgtcg gctgttttca tagccacttc cgcgcgtcca
     2341 tcgccgtcaa aatcgtacac ttggaattgg gtgtaatggg ccccagcgcg aatattgcgg
     2401 cccaagtcaa tgcgccatag ttgttgcccc tctagcgtgt aggcatcgat ataaacattg
     2461 cccgtattgc cggattggga attgtctttg gcgttggacg ggtcccattt cacgaccagt
     2521 tcgtaatcgc catccccatc caaatctcca actgaggtat cgccgggcga gtaggtatag
     2581 gccacgccat caggccctgt gccgccgctg gggcgtttga gggtaataat tttgctttgg
     2641 ctggcccata ccgatgcctg ctcagccgct tgttcacggc catcgatcac tgggcgcagg
     2701 ctgtattggc tgttattggt gcctgcgcta tcgacataat tggtgagagc actgatggct
     2761 tggccggtaa ttaaattgcc gtctcgataa atgttaaacg ctgtgttggc aggatcagtg
     2821 cccaatgagc gccaggataa aaatacgccg ttattggttt ttactgccac caaaccccgt
     2881 gagaggtttt ccatttgttt gccgttgttt gcggtttggc cagagctgct ggaagacgag
     2941 gagctagagg tgcgaccgct cgatgaactg ctggagcttg atgaactact agaagatgta
     3001 gagctggaag aactcgagga ggatgaactt gaactactgg atgaggtgcg gccggaacct
     3061 gaggagcttg agctggatga gctgctggag ccggaggccg ttccatccgg gcctgaacac
     3121 gcgcttatta acaggcaggt aaataaactg gtaaacaacg gcttgtgcat agcgcaatct
     3181 caacatgggt gagcggttag agaataatgt ggcagcacta aacctaaaaa ttttggccag
     3241 ctgtgttgtt ctattgagca ggcatttagg tttagataaa gactgcgcag caatatacag
     3301 ggataatagc ggcaggatct gaggtgcgat tcaaatttgc ggtaaaagga ttgtggcaac
     3361 tgtttacctg gttggcttag cgtagcagcg ttaatttgtc acaattattt ttgccaaagc
     3421 gatgcgtgaa ctcgcccagg cctaattatc aaccctgcga tatttaatgc cgggcaaata
     3481 gaaactccta taaaagatcc gtattctccg ctaactatag gtattcaccg tggataaaaa
     3541 ccgaatagaa gcatttagtg atggcgtgtt ggcgattatc atcaccatta tggtgttgga
     3601 actaaaagtg ccccatggta ttgagttgtc agcgcttatc cagctttacc ccgtatttat
     3661 aagttactgc ctgagtttta tttacgtggg gatttattgg aataatcacc accacatgtt
     3721 taaagcggtg cacaaagtca atggcgctgc gctgtgggct aatttagtgt tgttattttg
     3781 gctgtcgctt ttacccttta ccaccggctg gatgggggaa aatcattttg cgcagtggcc
     3841 ggtgacggtg tacggaatta acttgctctt ctgcgcactt gcttattttg tgctgcaaaa
     3901 tgtattgatc cgccatcagg gcgccgatgg ggtattggcc cgtgccatag gcagagatat
     3961 caagggcaag ctctcccccg tgttttatat attagggtgc accgccgcct ggttgagtta
     4021 cccagcgtta ggcatggtat tttttgtcgc cgtagccttg atgtggctgg tgcctgatcg
     4081 ccgcgttgaa acgctggttg caaaaacccc tcatgtccat gaaggtgaat cgcgctgaat
     4141 taatttggta actattcagc tcactactcg ctgcgcgcca gtggcgtatt tttatgcaga
     4201 gctagctttt caatacgctg ttaagtcggg ctaaccgtac ccatggtttt ggctttgcgt
     4261 aacacatcgt caaaactcca gtggtcactg ccgtgccgcc cgtagtgcgc ggcaataatt
     4321 tcaccctttt cattaattaa aaattcggcg ggcaaccctt gtacgctttc ctctgccgag
     4381 ggcgcgcccc taaaaccttt tatgtacgca taaatagccg taaataatgc caccgggtgg
     4441 gccaccgccc aaatggattc ttgcaccgcg aactgcctat atagctgttt agtagggtcg
     4501 gcgatcatgg cgaagggcgt atcggcttga aattccttta aactctccac ggttgaatga
     4561 aacaccacaa cttcttgaat atttgcagcg atcagttctt gggcgcgggt tttaaattgg
     4621 tgtaaatgca aattgcacat ggggcagcct gcaaagcggc gaaactgtaa atgggtgaca
     4681 acacccgggc tggggatgct aatggcaccc tgattgacac tatcaagttt tatagagggt
     4741 aaaaaattgc ccggtttaaa gcgtcgcata gggtgttctc gtcatatacg gtggggtaaa
     4801 ccgacttccc tgtcggcagc ggcggggatt ata
//
LOCUS       DAOQGS010000308         4832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9204_length_4832_cov_3.776638, whole genome shotgun sequence.
ACCESSION   DAOQGS010000308 DAOQGS010000000
VERSION     DAOQGS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9204_length_4832_cov_3.776638"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKZ52_11705"
     CDS             <1..818
                     /locus_tag="PKZ52_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNG60481.1"
                     /translation="DGRLNEVFGHLDINTLRPEWMLQYFEARSSQISAKKELKFLSVL
                     CNWAKARGLMTAPNPTNDIMRHMKINEKRDIYVEDSWFDLVLKHSTPMVRDTLHFTYL
                     CANRPDETAQARFSDIDGDELVIALTKTENKGLAEKRIPITGELKSYIDSQRAKPIRS
                     MFLVSDSEGQQIKINTGKFKREFKLARDAAQVEADDIGVSFVRFQLKDIRAKAGTDLA
                     REYGIEAARLALGHTTQKQTQDYIRSVRKAGANARKSKIGESKIFSGESKSVR"
     gene            complement(838..914)
                     /locus_tag="PKZ52_11710"
     tRNA            complement(838..914)
                     /locus_tag="PKZ52_11710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Arg,seq:tct)
     gene            complement(932..1008)
                     /locus_tag="PKZ52_11715"
     tRNA            complement(932..1008)
                     /locus_tag="PKZ52_11715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(972..974),aa:Pro,seq:tgg)
     gene            1348..1746
                     /locus_tag="PKZ52_11720"
     CDS             1348..1746
                     /locus_tag="PKZ52_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG60482.1"
                     /translation="MTTDDNHTTRLNASLPETHATHVLSIEFFYAGKTYPVNMNKLPF
                     MIGREEQTCDLVIDSPMTSRNHCSIEIRENQLGLLDKSTNGTFVKLGRSDSVFIRNEF
                     CPLVGQGYIKLGEALSLDDPQLLMFKVVSR"
     gene            complement(1788..2312)
                     /locus_tag="PKZ52_11725"
     CDS             complement(1788..2312)
                     /locus_tag="PKZ52_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG60483.1"
                     /translation="MNVDAAWILIDTTLKVGLGGVIAISAAWLFQRSRHIKQLPQVAQ
                     RKLEILENISGSVGQVNHHFAKYSALVIESTRFGNAWPSARRLELESINEVLVKEFDQ
                     LADAEAKLLMLGEKTMERTLRLYAAKIALFRKQVYVGRADISDQEIAELKSGIMKLRE
                     QFYELLSKRYDRLL"
     gene            complement(2647..2901)
                     /gene="moaD"
                     /locus_tag="PKZ52_11730"
     CDS             complement(2647..2901)
                     /gene="moaD"
                     /locus_tag="PKZ52_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNG60484.1"
                     /translation="MSRPTRIVYFAALAQSLGVRDEQLNLAAAVSLAELRAQLIQRGG
                     PWQALAAPHIKAAVNHTMASGEAWVNPGDEVAFFPPVTGG"
     gene            complement(2898..3398)
                     /gene="moaC"
                     /locus_tag="PKZ52_11735"
     CDS             complement(2898..3398)
                     /gene="moaC"
                     /locus_tag="PKZ52_11735"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNG60485.1"
                     /translation="MSTNAFTHLDNQGQAHMVDVGAKPATHRQARAEGLVRMQPATLR
                     LIQEGGFAKGDVLAVARIAGIQAAKKCADLIPLCHPLLIHHVSVDIDLDEGASCARIA
                     ATVGLHGQTGVEMEALTAVSVAALTLYDMCKSVDKTLQIDGVHLAAKTGGRSGDWFFT
                     PVSAQP"
     gene            complement(3415..4440)
                     /gene="dapD"
                     /locus_tag="PKZ52_11740"
     CDS             complement(3415..4440)
                     /gene="dapD"
                     /locus_tag="PKZ52_11740"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNG60486.1"
                     /translation="MTQLYALGLGIGTQNNKQQWLEVFFAKPLLQPASQTAEALAKVL
                     GYSGGNAVIEFSAAKAEKLAQALEGSELFELAASLQHSSKPLVAVFLAENSGPKSVPE
                     AYLKLHLLSHRLVKPHETALDGIFAVLPNVAWTNQGAIDIEELPQRQLAARCKGEVLS
                     VDSVDKFPKMTNYVVPKGVRIAHTGRVRLGAYLGEGTTIMHEGFVNFNAGTLGTSMVE
                     GRISAGVVVGEGSDLGGGCSTMGTLSGGGNIIISVGKGCLIGANAGIGIPLGDRCTVE
                     SGLYITAGTKVALLDEHNTLVKHLKARELAGHSDLLFRRNSLNGAVECKTNRSAVALN
                     EALHANN"
     gene            complement(4451..4804)
                     /locus_tag="PKZ52_11745"
     CDS             complement(4451..4804)
                     /locus_tag="PKZ52_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HNG60487.1"
                     /translation="MAVRLFGIPACDTVKKARQWLDAAGVAYEFIDLRQPPIAPEQVA
                     IWYGALGSSLVNTRSTTYRQLSPQEQAQIVQGDSLAVLQRHPTLIKRPVLQTEQAFYC
                     GFLAATYQSIFQHIN"
BASE COUNT         1229 a         1311 c         1231 g         1061 t
ORIGIN      
        1 atgatggccg cctaaatgag gtatttggcc acctggacat aaacaccttg cgtccagagt
       61 ggatgctgca gtatttcgag gcgcgctcct cgcagatttc ggccaagaag gaattgaagt
      121 ttttaagcgt gttgtgcaat tgggcgaagg cgcgcgggtt aatgacggcc cccaacccaa
      181 cgaacgacat aatgcggcac atgaagatta acgaaaagcg cgacatttat gtggaggatt
      241 cttggtttga cctagtgctt aagcactcca ccccaatggt tcgcgatacg ctgcacttca
      301 cctacctatg cgccaaccgc cccgacgaga ccgcgcaggc cagattctcg gatatagacg
      361 gggatgaatt ggtgatagcg ctcacaaaaa cggaaaataa gggattagcg gaaaaacgga
      421 taccgattac cggcgagctg aaaagttata tcgactcgca acgcgccaag ccgatccgct
      481 caatgttcct ggttagcgac tcggaggggc agcagatcaa aattaataca ggcaagttta
      541 agcgtgagtt caaactggca agagacgccg cgcaggtcga agctgacgat atcggggttt
      601 cgtttgttcg atttcagcta aaggatatta gggcaaaggc cgggactgat ttggcccgcg
      661 aatacgggat tgaggcagca cgtttggctc ttggacatac cacccagaag caaactcagg
      721 actacattag gtcggttcgg aaggctgggg caaacgccag aaaaagtaaa attggggaaa
      781 gtaaaatttt tagcggggaa agtaaaagtg ttcgctaacc gtgtaggcct cgccgtgtgg
      841 cgcgcccgag aggattcgaa cctctgaccc acggcttaga aggccgttgc tctatccaac
      901 tgagctacgg gcgcattcag acccggaaaa atggtcggag tggagggatt cgaacccccg
      961 acattctgct cccaaagcag acgcgctacc agactgcgct acactccgac attcacaaga
     1021 atacccgtgg gcacccctgg aagaggtgcg catattactg acgcccccct tgtgcgtcaa
     1081 tacttgcgct aaaaaaatgt ctcgctgttt cattggctta gctgcgccga gtgtttaagg
     1141 ttacaagcat ctagccgcag tgctaaaaca gcataatcac agcaaataaa acagatccat
     1201 tacaccgctt aatcacacct tttaggccta actgcatgca ataggccgca ataaaataag
     1261 cactatctgt gaagcaagtg ttcgctgacg ggttacgcaa aggcactaca cgggttagaa
     1321 ttatcgaatt caccacgagg ccgccttatg acaactgacg acaatcacac tactcggtta
     1381 aatgcgtctc ttccggagac ccacgccacc cacgtcttgt ctatagagtt tttttacgcg
     1441 ggcaaaacct acccggtgaa tatgaataaa ctgccattta tgattggccg ggaagaacaa
     1501 acctgcgacc tggtcatcga cagccccatg acctcacgca atcactgctc cattgaaatc
     1561 cgtgaaaacc aattgggttt gttggataaa tctaccaacg gcacctttgt aaaactcggc
     1621 cgctccgata gcgtatttat tcgcaatgaa ttttgcccgc tggttggcca aggttatata
     1681 aaacttggcg aagcgctgag tttggacgac ccacaattat taatgtttaa agtcgtcagc
     1741 cgataaattc atccgccttg gcgccataaa aataccggct aaaaacccta caacaagcgg
     1801 tcgtaacgct tactcaataa ttcgtagaac tgctcgcgca atttcataat tccggattta
     1861 agttcggcaa tttcctgatc gctaatatcc gcgcgcccca catacacttg tttgcgaaat
     1921 agcgctattt ttgccgcata taaacgcagg gtgcgctcca tggttttctc acccagcatc
     1981 aataatttag cttccgcatc ggccagctgg tcaaattctt ttaccaatac ttcattgata
     2041 gattccaatt ccaaacgccg cgccgagggc caggcattgc caaagcgggt ggattcaatc
     2101 accagcgctg aatatttggc gaaatggtga ttcacctgcc ccacactgcc ggaaatattt
     2161 tccagtattt ctaatttgcg ctgggccact tgtggcagtt gttttatgtg ccgactgcgc
     2221 tgaaaaagcc aggccgcgct gatggcgatc acaccaccca aaccaacctt taaagtggta
     2281 tcaattaaaa tccaggccgc gtctacattc ataacaaaaa gctcaatagg tcaaaataat
     2341 gacgaagatg ggtttcttct ggctattttg caaagagcac tccaatttca ctcaatcgtc
     2401 ttaaatgaaa aaatgatcgg atctgatgaa acggctttca aagaagcgaa caaggcggaa
     2461 tgcccgagtc gcccatgagg gggggaggga agtcataagg ttacgagcag ttagtagagg
     2521 caacccttgt ggttgcccag tcggctatca ctcagttttt aggcgggagc gggcaaccac
     2581 aagtagcgcc ccgaatagct attaaggata tccgcaactt aacgacaaaa agaagctggg
     2641 taaaccctaa ccgcctgtaa ccggtgggaa aaatgccacc tcatcgccgg ggttaaccca
     2701 agcttcgccg ctggccatag tgtggttaac cgccgctttt atatgggggg ccgccagagc
     2761 ctgccagggg ccgccgcgct ggatcagttg cgcccgcagc tctgccaggc taaccgccgc
     2821 cgcaaggttt aactgctcat cgcgcacacc caggctttgc gccagggccg caaaataaac
     2881 aatacgggtc gggcgactca cggctgcgcg cttaccggag tgaaaaacca atcgccggag
     2941 cggccaccgg ttttggccgc taggtgcacg ccgtcaatct gaagggtttt atcaacggat
     3001 ttacacatat cgtaaagcgt cagcgccgct acgctcaccg ccgtgagcgc ctccatctcc
     3061 acgccggttt ggccatgtaa acccaccgtt gccgcaatgc gcgcgcagct ggcgccttca
     3121 tcaaggtcta tatcaaccga cacatggtga atcaataaag gatggcaaag gggaattaag
     3181 tccgcacatt ttttggcggc ctgaatcccc gcaatacgcg ccaccgctaa tacatccccc
     3241 ttggcaaagc ccccctcttg gataaggcgc agggtggcag gctgcatgcg cactagcccc
     3301 tccgcccgag cctgccgatg ggttgcaggt tttgcaccca catccaccat atgggcctgg
     3361 ccctggttgt ccaggtgggt aaaggcgttt gtgctcatag cagctcgctc ttggctagtt
     3421 attggcgtgg agggcttcgt tgagggctac tgcactgcgg ttggttttac actcaacggc
     3481 gccatttaaa gaattgcggc gaaataaaag atccgagtgc cccgccaatt cacgggcttt
     3541 taagtgtttc accagggtgt tgtgttcatc taacaaagcg accttagtgc ccgccgtaat
     3601 gtacaggcca gactccacgg tgcagcgatc acccagcgga ataccgatac cggcgttggc
     3661 gccaatcaag cagcctttgc ccacggagat aataatgttg ccgccgccag atagggtgcc
     3721 catggtggag cagccgccgc cgaggtcaga gccttcaccc acaaccacac ctgcggagat
     3781 gcgcccttcc accatgctgg tgcccagggt gcccgcgtta aaattcacaa atccctcgtg
     3841 catgatggtg gtgccttcac ccaaataggc acccaagcgc acccggccgg tatgggcgat
     3901 gcgcacaccc ttgggcacca cgtagttggt cattttgggg aatttatcca cgctatccac
     3961 gcttaatacc tcgcccttac agcgcgcggc taactgccgt tggggcagct cttcgatatc
     4021 gatcgcgccc tggttagtcc aggctacatt gggcagtacg gcaaaaatgc catccaaggc
     4081 ggtttcgtgg ggtttaacca ggcgatggct taagaggtgc aacttaaggt aggcctcggg
     4141 taccgatttg gggccggaat tttccgctaa aaataccgcc accaaaggtt tactgctgtg
     4201 ttgcaggctg gcggccagct caaataactc gctgccttca agcgcttggg caagcttctc
     4261 ggctttagcc gcagaaaact caatcaccgc attgccgccg ctatacccta aaaccttggc
     4321 cagggcctca gctgtctggc tcgcaggctg caacaagggt ttggcgaaaa aaacctccag
     4381 ccactgctgc ttgttatttt gggtaccaat acccaaaccg agggcgtaaa gctgcgtcat
     4441 aacacgtacc ttaattaatg tgctgaaaaa tagattggta ggttgcggcc aaaaagccgc
     4501 aataaaaagc ttgctccgtt tgcagcaccg ggcgcttgat cagcgttggg tggcgctgca
     4561 acaccgccag gctgtcgccc tgaactatct gcgcttgctc ctgcggcgac aactgccgat
     4621 aggtagtact gcgggtattg actaaggaac tgcccagcgc gccgtaccaa atggccacct
     4681 gctcaggtgc gatgggcggc tggcgcaaat cgataaattc ataggctacg ccagctgcat
     4741 ccaaccactg gcgggccttt tttactgtgt cgcaggcggg aatgccgaat aaacggacag
     4801 ccatagtacc cctttaaaaa gccgaaagca ta
//
LOCUS       DAOQGS010000309         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9276_length_4808_cov_4.566800, whole genome shotgun sequence.
ACCESSION   DAOQGS010000309 DAOQGS010000000
VERSION     DAOQGS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9276_length_4808_cov_4.566800"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PKZ52_11750"
     CDS             <1..1306
                     /locus_tag="PKZ52_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60488.1"
                     /translation="DSLMIQFSALEREDDFGDLSDQIVSDEFINRTRRLNNKDIYSIG
                     FNYDLGNSVYLMGYQSKGRVSRTDLSEIIFPWGDSTRAELPYVGKPEVTMVFGRFNSM
                     FGDLVIGCKRIKIHENYRGTYTDVIDGVQQDPFFEELIPENNKIELCSADYQYLVDEF
                     YAEFSFDSVDVDNLNFASLDDSFFSGEMGFNCFSEIECKLVHKEFLVSPVYGFEFLGV
                     QTVDANYGRNDYQPFSSVKIDGAGVEGVWSTIALYGNFQRSNVSSPFYLARQTANPNF
                     EEKQYTFGLTATPKWIDTLGFKFSLNESTSLYEAGALSKSNIPDLFRTEIYELSVNQA
                     IWNDLVGNLSLSAIDQRYVIPKFGSEADFRSRVRFVNLDLSLKFEPCRLCEFQVYAIN
                     ILDNNKKYIEGYLADFASAEQAASLEKYFTERVFALSVKVSY"
     gene            complement(1312..1752)
                     /locus_tag="PKZ52_11755"
     CDS             complement(1312..1752)
                     /locus_tag="PKZ52_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60489.1"
                     /translation="MKLLHLTKIATLASLLALSNISCAADPVKPATTAPSATYPSTEP
                     TTTAPPRTTENTERPPTLSYTTDKEGLLIPVDKNGKPFEQCSQEGSRNTCPLYNYKVN
                     VREIKTTVITEVTYKINPDCVQVCFEYGTGKPGRCYTKCSSQLP"
     gene            2031..2267
                     /locus_tag="PKZ52_11760"
     CDS             2031..2267
                     /locus_tag="PKZ52_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60490.1"
                     /translation="MKILIALLLLALTACAAVAQKTRQAFFPVNQSKVLLPVLTSAFV
                     PGEMHVYLQLEVRYAVFFIPLMAPMRIRMFLFSH"
     gene            complement(2562..3089)
                     /gene="yjgA"
                     /locus_tag="PKZ52_11765"
     CDS             complement(2562..3089)
                     /gene="yjgA"
                     /locus_tag="PKZ52_11765"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNG60491.1"
                     /translation="MNEEHNHNPDEIELLSKTSMKKEMQKLQDLGIELTLLKPQKLAT
                     MPISDELRDAIELLHRLTHNEAVRRQKQFIGKLMRHEDTDAIEKAFNNIAEKQNREAR
                     LLKTIERWRDNLIAGEASVAESFIESFPNCDRQQLRQLIKGAKSEFGRPKPPTFTRKL
                     FTFLRDIITAEPEQN"
     gene            complement(3103..4470)
                     /gene="mgtE"
                     /locus_tag="PKZ52_11770"
     CDS             complement(3103..4470)
                     /gene="mgtE"
                     /locus_tag="PKZ52_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNG60492.1"
                     /translation="MHPITIEERQSATQEHLGRIRALMESGTFQQVRRMLKALTPVEI
                     ARLISSSPAPSRPLIWGFVDKDDAGEVLEELPADIQSEFLSQMNTKDLVAMTEGLDPD
                     DIVDILQNLPDQVTQEVLAAMSAQDRERVSVILSYDEDTAGGLTNTDTITVRPRFTLD
                     VVLRYLRRHDDLPPSTDALIVVNKKDEYLGLLPLSVLLTNDPTTTVREVMETDRDPIP
                     AALPEKEVAALFEQHDWVSAPVVDEQGKLVGRITIDDVVDVIRQNADHSLMGMAGLGE
                     EEDTFAKARHTIGRRFIWLTVNLATALISSSVINLFKDTIEKVVVLAVLGPIVASMGG
                     VAGSQTLTVVIRGMALGQIGQHNLNWLLSRELRVGLANGVLLALMLGLGASWWFQDVK
                     LGAILGLALIFNLCTAATFGALLPVFLKSLKIDPALAGAVTLTTFTDSIGYFAFLCLA
                     SIFLT"
     gene            complement(4586..>4808)
                     /locus_tag="PKZ52_11775"
     CDS             complement(4586..>4808)
                     /locus_tag="PKZ52_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNG60493.1"
                     /translation="RAAAKFANTANRFSSTVEVSCGGKTINGKSIMSLMLLAASQGTA
                     ITLATSGSDALEALEALLELIANKFDEGE"
BASE COUNT         1077 a          976 c         1327 g         1428 t
ORIGIN      
        1 agattcttta atgatccaat tttcagcgct agagcgcgaa gatgattttg gggatttatc
       61 cgatcaaatc gtgagtgacg aatttatcaa tagaactagg aggttgaata acaaggacat
      121 ttattctatt gggtttaatt acgaccttgg taattcggtt tatctgatgg gttaccaatc
      181 caaagggcgt gtctcaagaa cggatctttc tgaaataatt ttcccttggg gggatagtac
      241 tagagcggaa ttgccttatg tcggaaagcc tgaagtgaca atggtatttg ggcgttttaa
      301 tagtatgttt ggtgatcttg taattggatg taagcggatt aaaattcatg agaattaccg
      361 tggaacttat actgatgtta ttgatggagt tcagcaggat ccattttttg aagaattgat
      421 ccctgaaaat aacaaaattg agttatgttc tgctgattat cagtatttgg ttgatgagtt
      481 ctatgcggaa ttcagttttg attcggttga tgtagataat ttaaattttg catcgttgga
      541 tgattcgttt tttagtgggg agatgggttt caattgtttt tccgagattg agtgtaagtt
      601 ggttcataaa gaattccttg tatcacctgt atatggtttt gaattcttgg gggtgcaaac
      661 agttgatgct aattatgggc gtaatgatta tcagcctttt tcgagtgtga aaatagatgg
      721 ggcaggtgtt gagggggttt ggtcaactat tgcattgtat ggcaattttc agcggtccaa
      781 tgtttctagt cctttttatc ttgcgcgtca gacagcaaat ccaaatttcg aggagaagca
      841 gtatactttt ggtttaacgg cgacgccgaa atggattgat acccttggtt ttaaattttc
      901 cctaaatgaa agcacctctt tgtatgaggc gggtgcctta tcgaagtcca atattcctga
      961 cttgtttagg accgagatat atgagttgtc tgttaatcag gcgatttgga atgacttggt
     1021 tggcaatctg tcgcttagtg ctattgatca gcgttatgtg attccaaagt ttgggagcga
     1081 agcagatttt cgttcgcggg taagatttgt aaacttggat ttatctctca agtttgaacc
     1141 ttgcaggtta tgtgagtttc aggtttatgc tattaatatt ttggataaca ataagaagta
     1201 tattgagggg tatttggctg attttgcaag cgctgagcag gctgcgagct tagaaaaata
     1261 ttttactgaa agagtgtttg ctctttcagt aaaagttagt tactagagat tttatggaag
     1321 ttggcttgag catttcgtat agcatcgacc tggttttcca gtgccatatt caaaacaaac
     1381 ctgaacgcag tctggattaa ttttgtacgt aacctcagtg attactgtgg tcttaatttc
     1441 tctgacattt actttgtaat tgtacagtgg gcatgtattt ctgcttcctt cttggctgca
     1501 ttgttcaaaa ggtttcccat ttttatcgac aggaattagc aagccttctt tgtctgtagt
     1561 gtaactaagt gtaggagggc gctctgtgtt ttctgttgtg cggggtggag cggtagtagt
     1621 tggctcagtt gatgggtagg tggcagaagg ggcagtagtt gcaggtttaa ccggatcagc
     1681 cgcacagcta atattagata aagccagtag cgacgcgagg gtcgctattt ttgttaaatg
     1741 taataatttc atgttgactc ctaggtgatt ttttgaataa gggtacgctt agcgcagggc
     1801 tatattaact aaacttgcta aggttgcaag ttttttgatt gtgtgctttt gaatggtgcg
     1861 aggtgttagt ggggattatt tgctgtggca tttttttgtg aatcagtcgc agttttttag
     1921 ctgtttttat cctgtagtga tagctatctt tttgcccgtc accgggcttc gcccgttagt
     1981 attttgtcgg tgagtgttaa taattacaca ataaattttg cgcccgacac atgaaaatac
     2041 tgatcgcatt actcctgttg gcgttaaccg cctgcgctgc tgtcgcacaa aaaaccaggc
     2101 aggcgttttt ccctgttaat caaagtaaag tgctgctgcc tgtgctgaca tcggcatttg
     2161 tgccgggtga aatgcatgtt tacctccagc ttgaggttag atatgcagtt tttttcatcc
     2221 cgctgatggc gccgatgcgc attcggatgt ttttatttag ccattgattt tgctggaggg
     2281 tttttcttgg gcggatgcac ctgaaggtca ttttaacggt gcacttgagg agattctctc
     2341 tcttgaacct agtgctaagc taaatagtgt cagcgttttt gcatagccgc cccagcttta
     2401 aaattaactg aatttaagct gtggcgtgct atgagcgtgg cgaggcataa gggcttgatt
     2461 aagccccctg tggtcatagg ttggtagcag gaggctaaaa agcctgaacc tacaaaataa
     2521 aatcaaaggc taaaaaagtc aaagccggtt aataaggccg gttaattttg ctctggctcc
     2581 gccgtaataa tatcccgcaa aaaagtaaac aacttgcggg taaaggttgg cggtttggga
     2641 cggccgaatt cggatttggc gcctttgatt aactgccgca attgttggcg gtcgcagttg
     2701 gggaagctct caataaagct ctccgctacg ctggcttccc cggcgattaa attatcccgc
     2761 cagcgctcta tggttttaag taggcgggct tcgcggtttt gtttttcggc gatgttgtta
     2821 aaggcttttt caatggcgtc ggtgtcttca tgacgcatga gtttgccgat aaattgtttt
     2881 tgcctgcgca cggcttcgtt gtgggtgagg cggtggagga gttctatggc atcgcgcagc
     2941 tcatcgctga tgggcatggt ggcgagtttt tgcggtttga ggagggtcag ctcaatacct
     3001 aaatcctgca atttttgcat ttcctttttc atgctcgttt tgctgagcag ttctatttca
     3061 tccgggttgt ggttgtgttc ttcgttcatg ggggcattca ttttaggtaa ggaaaatact
     3121 ggctaagcat aaaaacgcaa agtagccgat ggagtcggta aaggtggtga gggttacggc
     3181 gcctgcaagc gccgggtcaa tttttagaga ttttaaaaat accggcagca gggcgccgaa
     3241 ggtagcggcg gtgcataaat taaaaatgag tgccaggccc aaaattgcgc cgagtttgac
     3301 atcttgaaac caccagctgg cgcccagccc cagcataagg gctagtaaaa cgccattggc
     3361 caggcccacc cgcaattccc gggataataa ccagttgagg ttgtgttggc caatttgccc
     3421 taaggccata ccgcggataa ccacggtcag cgtttgtgag cctgccacgc cgcccatgct
     3481 ggccactatg gggccgagca ccgccagcac gactactttt tcaatggtgt ctttaaatag
     3541 gttgatcacg ctggaggaga tcagcgcggt ggcgaggtta accgtgagcc aaataaagcg
     3601 gcggccgatg gtgtggcggg ccttggcgaa ggtgtcttct tcctccccta aaccggccat
     3661 ccccattaaa gagtggtcgg cgttttggcg gatcacgtcc accacgtcgt cgatggtgat
     3721 gcggcccacc agtttgccct gttcatccac tacgggggcg gaaacccagt cgtgttgttc
     3781 gaagagagcg gccacttctt tttccggcag ggcggcaggg attgggtccc ggtcggtttc
     3841 catcacctct cgcaccgtag tggtcgggtc gttggtgagc agcaccgata gaggcaagag
     3901 gcccaggtat tcatctttct tgttaacaac aatcagggcg tcggtgctcg gcggcaggtc
     3961 gtcgtggcgg cgcaggtagc gcagcactac atccagggta aagcgcggtc gcacggtgat
     4021 ggtgtcggtg ttggtgaggc cgcctgcggt gtcttcatcg taggagagaa ttacgctcac
     4081 ccgctcgcga tcctgggcgc tcatggcggc gagcacttct tgggtcacct ggtcgggcag
     4141 gttttgcagt atgtctacta tgtcgtccgg gtctaagcct tcggtcatgg ccaccagatc
     4201 cttggtgttc atttggctta aaaattcgct ttgtatatcc gcaggcagtt cttccagaac
     4261 ttccccggca tcgtctttat ccacaaagcc ccagattaaa ggccggctgg gggcagggga
     4321 ggagctgatc aggcgggcga tttccaccgg ggtgagggct ttgagcatgc ggcgcacctg
     4381 ttgaaacgtg ccgctttcca tcagcgcacg aatgcgcccc aggtgttcct gggtggcgct
     4441 ttgccgctct tcgatggtga tgggatgcat aaaacctcta ggatcaaccg agcgctaggt
     4501 gcgctctgag cagaaaaaac caggcatcct agatacctcg cagggtgaac agggccgctc
     4561 aaaaagcgcg ctttgcaaag cggaattatt cgccttcgtc gaacttgttg gcgattaact
     4621 ccagcagggc ctcaagggcc tccagggcgt cgctgccgct ggtggcgagg gtaatcgccg
     4681 tgccttggct ggcggccagc agcatgaggg acataatgct tttgccgttg atggttttgc
     4741 cgccgcagga gacctccacc gtgctgctaa agcggttggc ggtgttggcg aacttggcgg
     4801 ctgcgcgg
//
LOCUS       DAOQGS010000310         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9321_length_4795_cov_4.113291, whole genome shotgun sequence.
ACCESSION   DAOQGS010000310 DAOQGS010000000
VERSION     DAOQGS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9321_length_4795_cov_4.113291"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PKZ52_11780"
     CDS             complement(<1..459)
                     /locus_tag="PKZ52_11780"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60494.1"
                     /translation="MLGEHTILVVDDNSKTQHDISVVLRFLNEPVTTHSYLQWRQLHD
                     SLSSRGEEISCIVLGETYERLDLIAEIKRIKDRLPFTPIILLNDKNLEWPEDIEPTIK
                     RRVIAQLCNPPAYNPWVDALHRAQRFREALMAASKNNEKRPVHLFRSLVGT"
     gene            616..960
                     /locus_tag="PKZ52_11785"
     CDS             616..960
                     /locus_tag="PKZ52_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60495.1"
                     /translation="MKLKNMSKHQRPRPVSNSQGLGAGQITDGNILACEALLAREIEL
                     KQNNLTWPHSRWGSGVEDLSITAGVAGAAHGALLRFNPVLRLSVRFKDLCRRRTSVEN
                     TGFIWPVGLFIY"
     gene            969..1520
                     /gene="ampD"
                     /locus_tag="PKZ52_11790"
     CDS             969..1520
                     /gene="ampD"
                     /locus_tag="PKZ52_11790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNG60496.1"
                     /translation="MAFDISNGLIQPARWVASPNYSARPSAGDISLLVVHNISLPPGE
                     FGGGHVEAFFQNRLDTSLHPYFAAIAELQVSAHCLIDRAGLLVQFVNLYERAWHAGVS
                     SFEGRPNCNDYSIGIELEGSDELAFTAAQYQTLAALARALMAEFPLITPARIVGHSHI
                     APGRKTDPGPAFDWDYFHGILSP"
     gene            1689..2498
                     /locus_tag="PKZ52_11795"
     CDS             1689..2498
                     /locus_tag="PKZ52_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60497.1"
                     /translation="MAFLAILLALSAVQLFGVQPLGGSWQARIHKAAQTVAGAVWVIG
                     LPTLAVAAAYLWLAVHAWWLALPLAAAVLWLSIASAAHKQAVEQFINAGRAEDWGAAS
                     EVYRQLEGAALVAEGDWQALNKAVLERAAYLGFCQFFAVLFWFCLLGPAAALGYRLLC
                     ISAQHNNWPPLGRFLWYVEWPAVRLLGLTFAFTGNFLTGIQRWLSCLSCSQRSSAEII
                     THYVLGALGVMEGGRQTVDVTRRELFAMARLLRRSLWFWLGALALLALRGW"
     gene            complement(2680..4221)
                     /locus_tag="PKZ52_11800"
     CDS             complement(2680..4221)
                     /locus_tag="PKZ52_11800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60498.1"
                     /translation="MPSLSTLLRPYGDKSVISVFVLGISSGFPWVMIGSALSLWLKEA
                     HLSRSAIGFAGLIFSVYAINFLWAPLLDRYAPRLGPLGKRRAWIAICQLAIAICCWAM
                     GLFNPVDAAAALMAVALLLAFCSATQDVAVDAYRTDGLKLDDAKNLSANSVATMAGWW
                     TGYAGLGGLPLILSDHGWSWPQLYWLMAGIMLLLWAITGFLPDPPNRHVHQTDQAAYT
                     YPLPGLGKIKLAGLMLGPFLLILWGALAGAGISWALIPGLASDYLTALLIMLGLVNLI
                     LIIRALMQVPRPAQVATATPSDRLLHWLMTTIIEPLDEFFRRQGTRYALSLLAFMLVF
                     KLGEAMLGRMSIVFYKEVGFSNTDIAEYSKLLTWWATVLFAIPCGLLNARLGTFKGLF
                     LAGIFMAASNLMFSLIAIKGPDTFWLGAAVLVDGFTSAWATVAFVAFISAQCNQKFSA
                     TQYALFASIGNFGRTNIAANSGVLVTALGGNWALFFILTSLMVIPGLWILWRLRNALE
                     GAVKT"
BASE COUNT         1045 a         1205 c         1393 g         1152 t
ORIGIN      
        1 ggtgcccact aaggagcgaa ataaatgcac cgggcgtttt tcattgtttt tactggcggc
       61 cattaaggct tcgcgaaaac gctgggcgcg gtgcaatgcg tccacccagg ggttgtaggc
      121 gggtgggttg cacagctgag caataacgcg gcgtttaatc gtgggctcta tatcctccgg
      181 ccactccagg tttttatcat tcaacagaat aatcggcgta aacggcaggc ggtctttgat
      241 gcgtttaatt tcagcaatta aatccaggcg ctcgtatgtt tcgcctaata caatacagct
      301 aatttcttca cctcggctgg agagggaatc gtgcaattgc cgccattgta aatagctgtg
      361 tgtggtgacg ggttcattta aaaagcgcaa aaccacactg atatcgtgtt gggttttact
      421 gttgtcgtct acgactaaaa ttgtgtgttc tcccagcata gccgtgtggt gcctcattat
      481 tttttgttat tggttgtggc tggtgtcata gcacaagcac tgccgcaggc ttattcctat
      541 tgaagattcg aggcttatta caggtaaatg caatatccaa gcaaccctat aggttaaaac
      601 gcgctgaaag gggctgtgaa attgaaaaat atgtcgaaac atcagcgccc taggccagta
      661 agtaatagtc aaggtttagg cgcaggtcaa attactgacg gcaacatatt ggcgtgtgaa
      721 gccttgctgg cgcgtgaaat agagctaaag cagaataact tgacctggcc gcactctcgt
      781 tggggttcag gtgttgaaga cttgagcatt actgctggcg tagcgggggc ggcgcatggt
      841 gcactattaa ggtttaatcc tgtccttcgc ctatcggtgc gctttaagga tttgtgccga
      901 aggcgtacct cggtggaaaa taccggtttt atctggccgg ttggcctatt tatctactga
      961 gaatgcccat ggcgtttgat atttctaacg ggcttattca gcctgcgcgt tgggtggcct
     1021 cacccaatta ttctgcccgc ccgagcgctg gtgatatcag ccttctggtg gtacataaca
     1081 tcagtttgcc acccggtgaa tttggtggcg gtcatgtgga ggcttttttt caaaaccgct
     1141 tggacaccag ccttcaccct tattttgcgg ccatcgccga gctgcaggtg tctgcccact
     1201 gtttaatcga tcgggccggt ttactggtgc agtttgttaa cctgtacgag cgggcctggc
     1261 acgcgggtgt atcgagcttt gaagggcgcc ctaactgtaa cgattattcc attgggattg
     1321 agcttgaggg ctccgatgag cttgcgttta cggccgctca gtatcaaacc ctcgcggcgc
     1381 ttgcgcgtgc cttgatggct gagtttccgc tgattacccc ggcgcggatc gtcggccata
     1441 gccatatagc gccggggcgc aaaaccgatc ccggcccggc gtttgattgg gattattttc
     1501 acggcatttt atccccctaa aaaacgcaaa gggccttcac agccaagggg gcgagcgctt
     1561 tttagcaaag tcttttacat ggctgtaagc cctggcttgc ggtttgctgc ccctgtagcc
     1621 attgcgtaca ctctgaatta agtattgttc tggttatccg ttttactagt tgcagccaag
     1681 gggcaaaaat ggctttttta gcgattttat tagcattaag tgcggtgcag ttgtttggtg
     1741 tacagcccct tgggggcagc tggcaggccc gcatccataa agctgcgcaa actgtggcgg
     1801 gggcagtttg ggtgataggg ctgcctactt tggcggtggc ggcggcctat ttatggttgg
     1861 ccgtccatgc atggtggtta gccttgccac tggctgccgc ggtgctctgg ttgagtattg
     1921 ccagcgctgc ccacaagcag gctgttgagc agttcattaa tgccggacgg gcggaggatt
     1981 ggggggcagc cagtgaagtg tatcggcagc tagagggcgc tgcccttgtg gccgaggggg
     2041 attggcaggc actgaataaa gcggtgctgg agcgcgcggc ctatctgggc ttttgccagt
     2101 tttttgcggt gttgttttgg ttttgcctac tcggccccgc tgcagcatta ggttatcggc
     2161 tgctgtgcat cagtgcgcag cataacaact ggccgccgtt gggcaggttc ttgtggtatg
     2221 tggagtggcc tgcggtgcgt ttgctggggc ttaccttcgc gtttacgggc aattttttaa
     2281 cgggcatcca gcgctggctc agttgcttat cctgctctca gcgcagcagc gccgaaatta
     2341 tcacgcacta tgttttaggt gcgctgggcg tgatggaagg cggccgacaa acggtggatg
     2401 taacccgccg cgagctattt gccatggcaa ggctgctgcg ccgcagcctg tggttttggt
     2461 tgggggcgct tgcgctgctg gctttaaggg gatggtaaat acttaataga attaatagct
     2521 tacagcatat tctaaaaata attgttaaaa gcttgaccgg ctcgcaaggc aagtcataag
     2581 atggccccac ctgttagccc ctgggcttgc ttaagtcagc tctcccctgt tgcatggatg
     2641 taacccgctc ccttgaacgc cctggggcta atgggccttc tatgttttta ctgctccttc
     2701 tagcgcattg cgcagccgcc ataaaatcca caatcctggt atcaccatca ggctggtcaa
     2761 gataaaaaat aacgcccaat tgccccctag tgccgtcacc aacacgccgc tgttggccgc
     2821 tatattcgtg cggccaaaat tgccgatgga ggcgaagagg gcgtattggg tggcggagaa
     2881 tttttgattg cactgggcgg agataaacgc cacaaaagcc accgtggccc atgccgaggt
     2941 aaatccatcc actaatacag cggccccgag ccaaaacgta tccggcccct tgatggcgat
     3001 gaggctgaac ataaggttgc tggccgccat aaatatgccc gccaaaaaca agcccttaaa
     3061 agtgcccagg cgggcgttga gcaggccgca gggaatggcg aataggacgg tcgcccacca
     3121 ggtgagcaat ttggaatact cggcaatgtc cgtattgcta aaccccacct ccttgtaaaa
     3181 aacaatagac atgcgcccca gcatggcttc gccgagctta aataccagca taaaggccaa
     3241 caggctcagt gcgtagcggg tgccctggcg gcggaagaat tcatctaaag gctcaataat
     3301 agtggtcatc agccaatgca gcagccggtc gctgggggtg gccgttgcaa cctgggcggg
     3361 cctgggcact tgcatcaggg cgcggatgat aaggattagg ttcactaagc ccagcataat
     3421 gagcagggct gtgaggtagt cactggcaag ccctggaata agcgcccagc taatgccagc
     3481 gccggccagt gcgccccaca aaatcaataa aaatggcccc agcattaagc ctgcgagttt
     3541 aattttgcca aggcccggta atgggtaggt gtaggcggct tgatcggttt ggtggacatg
     3601 gcggttgggt gggtccggca ggaagccggt gatggcccac agcaacagca tgatacccgc
     3661 catcaaccag tacagctggg gccagctcca gccgtggtcg gaaagaatca aaggcaggcc
     3721 gccgagcccc gcataacccg tccaccaacc ggccatggtg gccaccgagt tggccgagag
     3781 attcttcgca tcatccagct tgagcccatc tgtgcggtag gcgtccaccg ccacatcctg
     3841 ggtagccgaa caaaacgcca gcagtaacgc cactgccata agcgcggcgg cggcatccac
     3901 gggattaaat aagcccatgg cccagcagca gatggcaata gccagttggc aaattgcaat
     3961 ccaagcgcgg cgtttaccca gcggccccag ccgcggtgca tagcgatcaa gtaggggcgc
     4021 ccagaggaaa ttaatggcgt aaacactaaa aattaacccg gcaaagccta tggcgctgcg
     4081 gcttaagtgg gcctctttaa gccagaggct gagggccgag ccgatcataa cccaggggaa
     4141 accactgctg atgcccagga caaaaacact aatgacggat ttatcaccgt aggggcgcag
     4201 aagggtagac agcgagggca tggctggggg ctctgatggc aatcgcgcac actagcgggt
     4261 aaggggcaaa aattcaaaaa tactgcgcaa ccgtaaactg cggcttgcgc gcttcgggtg
     4321 atgcccctca ggcaatgcca tttaagttag ggattctggc gcgcaatttc ggtgtgagac
     4381 tacctagctg ggctacttaa ggggcaatta agcaggatcc tgggggcaac ccttgtggtt
     4441 gcccagttta tgcctctgcc ctgatcagga tttacgcggc agcgggcact caaggggcgc
     4501 ccccacggct tttccccatt gggagattca cattgatttg aggggggctt atcctcagct
     4561 taatgccatc gcctcgctca gatggagcct ggatgctaat tgttttcacc cctggcgctt
     4621 gaatttccct cgcggccccc aagatagcct gccattcgct gtaggtgaga cacaggttga
     4681 tgcgcaaaat tattggtatt tggttgggcc ttgaacttgt gttggcgtgg ctgattagcc
     4741 attgggtggg atttggcagc ctattgctga gctggttatt cgctattgcg gtggg
//
LOCUS       DAOQGS010000311         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9338_length_4788_cov_6.567716, whole genome shotgun sequence.
ACCESSION   DAOQGS010000311 DAOQGS010000000
VERSION     DAOQGS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_4788_cov_6.567716"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PKZ52_11805"
     CDS             complement(<1..1151)
                     /locus_tag="PKZ52_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60499.1"
                     /translation="MAGKALPPSGGGYDPKFKALLDKLSERVDVLSDHRGDPLDRALT
                     PRDLLDLGLAKRIGRAAQRLVSGSNLVATTGPGSSAIPPAPTGFTVTGGLSHIFLEWD
                     NAREAYGNHGYTAIFRNSVDNLANAVEVAQTASSFNFADMDVHFGVNYYYWIRFVSAS
                     NVMGPPNSPAGTLGKVAEDPAELLERLSGEIGRTQLAEALRTEIDDIADDIAALEAVY
                     GDTATAAQAAADAAAAATQALGARADAVQARTDAIAAQTAAVNAKNIAIVEAGNALTK
                     ANEASASALTASTAGATATSKAAEAATSATSSAGSAASAQSSAELAATSATASGNSAT
                     AAASSASSASSSATAAGTSASAANTSKLAAETALAGAQSAQSSAVSAKN"
     gene            complement(1151..2734)
                     /locus_tag="PKZ52_11810"
     CDS             complement(1151..2734)
                     /locus_tag="PKZ52_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60500.1"
                     /translation="MSISLGPFLGLMPKADPKLLNPQFAQVALDCDLSTGSLAPARAP
                     GASIGALLSGTKTLHWFNPASNGGAGYWLQFSGDVDVVRGPIADDPYLRTYFTGDGVP
                     KYTTVTLAQQGAGPYPGASRNLGLPAPSAPTAAGPSGTPPPGGQKIQTAYVMTYVSDI
                     GEEGPPSEPSNIIDRWDGGTVNLTGLSVASGSFVIVAKRIYRIELNDVYQFVGEVGAA
                     TTTFTDNFDTEFLGEPVPSSEWVAPSPDMKGLVVLPGGIMAGAWGNTIAFSEPYQPHA
                     WPIRYRLALDYDVVAMAVSAYGLVVGTTGTPYLVSGSSPDAMGQTKLELAAACVSKRS
                     MVDMGDFVVYATADGLVGAGGNGARLITAEHITPKQWRTRYNPALIHAVRWGERYLGF
                     YPGGAFTFSPSEGFKDFTQTADCTFLDEAKGDIYIKTGVQLRKWDEGGATSYTYRTKI
                     FSIPPSRPFMACKVSASSYPVTLKFYADGVLKKTKAVQNDAAFRLPANKRYSECEIEL
                     TGTATVSGLQVAATMSEIV"
     gene            complement(2751..3980)
                     /locus_tag="PKZ52_11815"
     CDS             complement(2751..3980)
                     /locus_tag="PKZ52_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNG60501.1"
                     /translation="MPRPATSFNKRIVILGSSVAAGSGASYGWGARLASALLALGYEV
                     INVSISGNKTSDCINRFYSDVAPLNPYAVIIGLSLANEGLASLANHEAVYRSFINGIS
                     QLIEMNRQIGAHTIIGGVYAQNVYTAKEYYYLKLADSQLSKIEGVPYIPFLGQIDDGS
                     GHWESGMFTDAGHPNDIGAESMFRAFDLKLFNNLNRYRAALNVVNGWALKLPSTAGAM
                     PFYYQPDTPFGCVTVMFKLRFGAASLIGQPICSLVGSFAIPLRIKNQLGNYEVAAGYT
                     TIIDSGIASTGPDARWLTIVFDYHVNVAKLYVDGVFSGQAVAAGASDITAVAWAQRPD
                     SLGFDGLDYEYSQFMVWRTALTADQVLAVTRGEIYTGGLVVYSPCADGLALQDARLTN
                     LAPTDGAILLGAQPALV"
     gene            complement(4350..>4788)
                     /locus_tag="PKZ52_11820"
     CDS             complement(4350..>4788)
                     /locus_tag="PKZ52_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60502.1"
                     /translation="MSGITLFIVSVIAIALLAIAFFVKRPTQYFKSGEGKGVLFGIAA
                     AIGACVSVALFAFSLKSQALEYFKSGEVYLGLDATNKISPMCYAGENSDRLTSNLGVR
                     ASLIRSDDGQASVNFKYTHHSCAFNADNRAYDALGLEAVYKIW"
BASE COUNT         1150 a         1346 c         1256 g         1036 t
ORIGIN      
        1 tcgtttttgg ctgaaaccgc agatgattgt gcggactgag cacccgctaa cgccgtctct
       61 gccgcaagct tcgatgtgtt ggcagcactt gccgatgttc cggctgcggt ggcgcttgat
      121 gatgcactgc ttgcgcttga cgcggctgca gtggctgaat tgccagatgc tgttgcgcta
      181 gtggcggcaa gctcggcgct tgactgcgca gaggctgcgg aaccggccga actggtggcg
      241 ctagtagccg cctcagccgc cttgctggta gccgttgctc cggccgtgga tgctgttagg
      301 gcagatgccg acgcctcgtt ggccttggtg agagcgttgc ccgcctcaac aattgcaata
      361 tttttagcgt ttaccgctgc ggtctgcgct gctatggcat ccgttctagc ctgaacagca
      421 tccgccctcg cgcccaaggc ctgagtggcc gccgccgccg catccgccgc cgcctgggcg
      481 gcagtggcgg tatcgccata caccgcttca agcgccgcaa tatcgtcggc aatgtcgtct
      541 atttcggtgc gcagcgcttc ggctaattgg gtgcgcccaa tctccccact aagcctttcc
      601 agcagctcgg caggatcttc cgccaccttg cccagggtgc cagcagggct gttgggtggc
      661 cccatcacat tggaggcact gacaaaccga atccagtagt aatagttaac cccgaaatgc
      721 acatccatat cagcaaagtt aaaactgcta gcggtttgcg ccacctctac ggcattggcc
      781 aggttatcaa ctgaattgcg gaatatggcg gtatagccgt ggttgccgta ggcctcgcgg
      841 gcgttatccc actctaagaa aatgtgcgaa aggccgccgg taactgtaaa accggttggc
      901 gccgggggaa tggcagaact gccggggcca gtggtggcca caaggttgct accggacacc
      961 aggcgctggg ccgccctgcc aattcgcttg gcaagcccaa ggtccagcag gtcgcgagga
     1021 gtgagcgccc tatctagcgg gtcgccccgg tgatccgaaa gtacatctac ccgctcgctc
     1081 agcttatcga gcagggcctt aaatttgggg tcataccccc cgccgcttgg cggtaatgct
     1141 ttgccggcca ttagacaatc tcgctcatag tggcagccac ctgcaggccg ctcacagttg
     1201 ccgtgccggt tagctcaatc tcgcactcgc tgtagcgctt attggcgggc aagcggaagg
     1261 cggcatcgtt ttgcacggcc ttggtctttt tcagaacgcc gtcggcatag aatttcaggg
     1321 taacgggata gctggatgca gataccttgc aggccataaa cggccgcgat ggagggatgg
     1381 agaaaatctt ggtccggtag gtgtagctag tagcgccgcc ctcatcccac ttgcgcaact
     1441 ggacgccggt ttttatgtag atatcgcctt tggcctcgtc cagaaatgtg caatcagcag
     1501 tctgggtaaa atccttaaat ccctcgctgg gtgaaaacgt aaaagcgcca cccgggtaaa
     1561 aacccaaata ccgctcaccc catcgcacgg catgaatgag cgcggggtta tagcgggtgc
     1621 gccattgctt gggcgtaatg tgttcggcgg ttattagtct cgcaccattc ccgccagcac
     1681 caaccaggcc gtcagccgtg gcatacacaa caaagtcgcc catatcgacc attgagcgct
     1741 tcgatacgca ggcagcagcc agctccagct tggtttgccc catggcgtca gggcttgagc
     1801 cggacaccaa ataaggtgtg ccagtagtgc ccacaacaag cccataggct gatacagcca
     1861 tggccaccac gtcataatcc agcgccaggc gatagcgaat aggccaggca tggggctgat
     1921 agggctcgct gaatgcaatc gtattgcccc aggccccggc catgattcct ccaggcaaaa
     1981 ccaccaggcc tttcatatct gggcttggcg caacccactc agagcttggc accggctcgc
     2041 ctaaaaactc agtatcaaag ttatcggtaa aggtcgtagt ggccgcgcca acctctccca
     2101 caaactgata aacgtcgttt agctcaatgc ggtaaatgcg cttggccaca atgacaaaag
     2161 agccagacgc aaccgataac ccggttaaat tcaccgtgcc gccgtcccac cggtcaatga
     2221 tattgctagg ctcactgggc ggcccctcct cgccaatatc gcttacatag gtcatgacat
     2281 aggcggtttg gattttttgg ccaccagggg gcggagttcc acttgggcct gcagcagtag
     2341 gcgcagaggg cgcgggcaat cccagattgc gcgaggcgcc agggtatggc cccgccccct
     2401 gttgcgccag cgtcacagtg gtgtactttg gaaccccatc gcctgtgaaa taggtgcgca
     2461 aataggggtc atctgcaatc ggcccccgca ccacgtccac atcgccggaa aactgcagcc
     2521 aatagccagc gccgccgtta gaggccgggt taaaccaatg gagtgtttta gtgccgctga
     2581 gcaaggcccc aatggatgca ccaggggcgc gggcaggagc aagcgaccct gtacttaaat
     2641 cacaatcaag cgccacctgg gcaaattgtg gatttaataa ttttgggtca gccttcggca
     2701 ttaagccaag aaatgggcca agcgatatac tcatggatta ccttggagga ctatacgagg
     2761 gcgggctgag cgcccaggag aattgcgccg tcggtgggcg ctaggtttgt cagtcttgcg
     2821 tcctgaaggg caaggccatc ggcacaaggg gaataaacaa ctaagccgcc ggtataaatt
     2881 tccccgcgag ttaccgccaa cacctgatcg gccgttagcg ctgttcgcca aaccatgaat
     2941 tgagaatatt cataatccag gccgtcaaag cctagactat caggcctttg agcccacgca
     3001 acggcagtta tatcactggc accggcagcc actgcttgcc cagaaaaaac gccatccaca
     3061 tagagttttg cgacgtttac gtggtaatca aaaacaatgg tgagccatct cgcatccggc
     3121 ccagttgagg caatgccgga atcaattatg gtggtataac ctgcagcaac ttcgtaattg
     3181 ccgagctgat tttttattcg caatggtatg gcaaacgacc caaccaagga gcaaatcggc
     3241 tggcctataa gacttgcagc cccgaacctc agcttaaaca tgacggttac gcaaccgaag
     3301 ggggtatccg gctggtaata gaacggcatt gcgccagccg tagaggggag cttcagcgcc
     3361 caaccattaa caacatttaa cgcggctcga taccgattta aattattgaa taattttaaa
     3421 tcgaaggccc taaacatcga ctcggcacca atatcattag ggtgtccagc atccgtaaac
     3481 atgccagact cccaatgccc tgagccgtcg tctatttgtc cgaggaacgg aatataaggc
     3541 acaccctcaa ttttgctcaa ttgcgagtcg gccaatttta aatagtaata ttccttggcc
     3601 gtgtagacat tctgagcgta cacaccgcca ataatcgtgt gcgcgccaat ctgccgattc
     3661 atttcaatta gctgcgaaat accattaata aatgatcggt aaaccgcctc atggtttgcc
     3721 aagctggcaa gcccctcatt cgccaggctt agcccgataa ttaccgcata cggattaaga
     3781 ggcgcaacat ccgagtaaaa ccggttaatg cagtcgctag ttttatttcc gctaatcgaa
     3841 acattaatta cctcgtaacc cagtgccaat agcgcgctgg caagtcttgc gccccagcca
     3901 taggaagcgc cagagccagc agccacggag cttcccaaaa ttacaatacg cttgttgaat
     3961 gaggttgcag gccttggcaa ataggtgctg ccaggcggga gatttataag gtcgcgaata
     4021 tcggcatttt cgccgcctac aataaattcc tcgccggaat gaatgtggcg cccagtaatc
     4081 gggtgccaac tttttatctc atagtgcgta tcgctgtacg cgccctcatt ttgccaaagc
     4141 gtaaacgtgc acacacctga attattggtg agttgcgatt ttatattggg cgcaacaaaa
     4201 ccacctgtac ctttgccaag gccaaccaac ttaatactca ccttggcgcc agccagggga
     4261 gcaccatcaa aatcacttat ggttactgat acttgtcgag tctgaatcac agagcaccca
     4321 cttttttaat atttattaag cgcgagcgct caccagattt tataaacagc ctctaacccc
     4381 agcgcatcgt aggcccggtt atcggcgtta aatgcgcagg agtggtgtgt gtatttaaag
     4441 ttaacgctcg cctgcccgtc gtcacttcta ataagcgatg cgcgcacacc cagattcgag
     4501 gtaagccggt cggaattttc gcccgcgtaa cacatggggc taatcttatt agtggcatct
     4561 aaccccaaat acacctcgcc ggatttaaag tattccagcg cttgggattt aagcgagaat
     4621 gcgaatagag caactgaaac acaggcgcca attgcggcag caataccaaa taaaacgccc
     4681 ttgccttccc cggatttaaa atactgagta ggccgcttaa caaaaaacgc tatcgccagt
     4741 agcgcaatgg caataacgga aacgataaaa agagtaatgc cggacatg
//
LOCUS       DAOQGS010000312         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9344_length_4785_cov_4.738478, whole genome shotgun sequence.
ACCESSION   DAOQGS010000312 DAOQGS010000000
VERSION     DAOQGS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9344_length_4785_cov_4.738478"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..712)
                     /locus_tag="PKZ52_11825"
     CDS             complement(62..712)
                     /locus_tag="PKZ52_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG60503.1"
                     /translation="MSTVLEVNIQFQRGSHRLIANACINAPITGILGASGSGKSTFLS
                     AIVGLIKPHQGIIKLNGKTLFNASSREWVPPHKRRIGLVFQDGQLLPHLSVKRNLLYG
                     YHNTPHEERRFTLDSVVELLELAPLLNRHPKALSGGERQRVALGRAVLYSPHLLLLDE
                     PLSALDERLKQQILPFFARIYSECTIPMIYVSHAQSEIDYLTDTCLQVADGELKSL"
     gene            complement(696..1391)
                     /gene="modB"
                     /locus_tag="PKZ52_11830"
     CDS             complement(696..1391)
                     /gene="modB"
                     /locus_tag="PKZ52_11830"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNG60504.1"
                     /translation="MLTDAEWQVLGLSTKVGLCATLLCLLPGIFFGWLLARRDFWIKP
                     VLEAALFMPMVLPPTVPGYLLLITFGNQGAIGRWLKEHFNLEFAFNWKGAVLASAVIA
                     FPLMVQAAKLSVRMIDRRIELAASTLGASPFKVWYTVTLPLMLPGILIGSIMVFSRSL
                     GEFGATITFVGNIAGETRTLPLAIYSATHQIDGDKDAMRLIFICMALAFCSLLASNVL
                     TAKVERWLGEHRA"
     gene            complement(1438..2247)
                     /gene="modA"
                     /locus_tag="PKZ52_11835"
     CDS             complement(1438..2247)
                     /gene="modA"
                     /locus_tag="PKZ52_11835"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNG60505.1"
                     /translation="MFPLLKQPHPNRNRLRCQLYASVLIAVNCFLLPPGAYAAEVKVY
                     AAASLTDALSELAQAYQKSTPASSIKNSFASSATLAKQIENGANADVFISADKDWADY
                     LEKRNLIDKSSRTNLLANQLVLIAPLNASPQVTLSKGVDLAKAFNGKLCTGDPTSVPV
                     GKYAKQALDYFNSWKTIESRVVGTEDVRTALAFVERGECGLGIVYSTDAQISKKVHIV
                     ARFPSESHAPIIYPGALTTKANAEAKQFWHYLQTPAAAEVFKKYGFQIDAK"
     gene            2813..2938
                     /locus_tag="PKZ52_11840"
     CDS             2813..2938
                     /locus_tag="PKZ52_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60506.1"
                     /translation="MDFLVLTQLAAVCTAERGILAASINPALNAAGLILTLRMDV"
     gene            complement(3113..3400)
                     /locus_tag="PKZ52_11845"
     CDS             complement(3113..3400)
                     /locus_tag="PKZ52_11845"
                     /inference="COORDINATES: protein motif:HMM:NF039672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HNG60507.1"
                     /translation="MNHTPNIRFFISYSGMKLPLNLVNEISPEQMHNRNTYFKATYDE
                     EGKMLLCQKIVYNEVDVQHEYEYSQDGILIFARITEGDEIRELNLATSGKL"
     gene            complement(3397..4134)
                     /locus_tag="PKZ52_11850"
     CDS             complement(3397..4134)
                     /locus_tag="PKZ52_11850"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNG60508.1"
                     /translation="MSAKNSRSKTQDKWQHHLQNTWQDKWRRNDIAFHQPQVNPLLQQ
                     YLPSLKLCPGDQVLVPLCGKSVDMDWFIQQGLSVIGVEVSAVAIAAYFATLAITPKQQ
                     VQGNFIRWQFEQRQILCGDIFDLRLRDLNKIKLVYDCAALTALPPQLRILYVAHLRRT
                     LPSSSPILLMTVESSETNLTALDALPSAQRIDSEVSALYQKEYQIKLLHGASCVRIDP
                     EHPSIGPCALDEKVYLLNKLAKPLNTP"
     gene            complement(4233..4640)
                     /locus_tag="PKZ52_11855"
     CDS             complement(4233..4640)
                     /locus_tag="PKZ52_11855"
                     /inference="COORDINATES: protein motif:HMM:NF022495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HNG60509.1"
                     /translation="MPHSPLVCELAALNSMTDTERLEYFMTRVFEAEEVWYLSSANRF
                     FTRTINGEHYFPLWPYKIFATEAALDYWHDGAPAACSLEYFMEEIIEQLHAQNILLDV
                     MPRAEEPGSLISPKRLQAILQGMIDAGEYRLDS"
BASE COUNT         1168 a         1058 c         1219 g         1340 t
ORIGIN      
        1 gccaaattcg cctagctagt gcttcgccgc ctacattttt cagaggttcc ctagttattg
       61 gttaaagcga ttttaattcg ccgtctgcca cttgtaggca ggtatcggtt aaataatcaa
      121 tttcactttg ggcgtggctt acgtaaatca tagggatagt gcattcgctg tatatgcgcg
      181 caaaaaacgg caaaatttgt tgttttaagc gctcgtctaa ggcggataaa ggctcatcca
      241 ataataataa gtgcggtgaa tacaacaccg ctcgtcccag tgctaccctt tgccgttcgc
      301 caccggagag cgctttgggg tggcggttta ataagggggc cagctctaat aattccacta
      361 cagaatccag cgtaaagcgg cgctcctcat gaggggtgtt gtggtagccg tataataaat
      421 tacgttttac cgacaggtgc ggcaacaatt gaccgtcttg aaaaaccaag cctattcgcc
      481 gcttgtgggg tggcacccat tctcggctgc tggcgttaaa aagtgtttta ccattaagtt
      541 taataatgcc ctggtggggt ttaataaggc ccactatggc cgataaaaag gtacttttgc
      601 cactgccaga tgcacctaaa attccggtaa ttggcgcatt gatacaggcg ttggcaataa
      661 gcctgtggct gccgcgctga aattgaatat tcacctcaag cacggtgctc acccagccag
      721 cgctctactt ttgcggttaa cacattgctg gctaacagcg agcaaaaggc caatgccata
      781 cagataaaaa ttaaacgcat agcgtcttta tcgccgtcga tttggtgcgt agccgaataa
      841 attgccaggg gcagggtgcg ggtttcacca gcaatattgc ccacaaaggt tatggtggcg
      901 ccaaattcgc cgagggagcg gctaaatacc ataatagagc cgattaaaat acccggcagc
      961 atgagcggta aggtgacggt gtaccagact ttaaaagggc ttgcgcctaa tgtgctagcg
     1021 gctaattcaa tgcgcctgtc aatcatgcgt acagataatt ttgccgcctg caccattagg
     1081 ggaaaggcga taaccgccga tgccaacacg gcgcctttcc aattaaaggc aaattccagg
     1141 ttaaaatgct ctttaagcca gcggccaatg gccccttggt tgccaaaggt aattaataat
     1201 aaataaccgg gcaccgtggg cggcaatacc atgggcataa ataaggctgc ctccagaacc
     1261 ggttttatcc aaaaatcccg gcgcgctaat aaccagccga aaaatatacc gggcaataaa
     1321 cacaacagcg ttgcacacag gccaaccttt gtggataggc ctaatacttg ccattcggcg
     1381 tcggttaaca tcgggcaggg gtgtccttat tgttcagggc cgtttgtcag tgggccatta
     1441 tttggcgtcg atttgaaaac catatttttt aaatacctcc gctgctgctg gggtttgtaa
     1501 atagtgccaa aattgttttg cttcagcatt ggctttagta gtgagtgcgc cgggataaat
     1561 aataggggca tgactttcgc tggggaagcg cgccacaatg tgcacttttt tactgatttg
     1621 ggcatcggta ctgtacacaa tacccaggcc acattccccg cgctccacaa atgctaatgc
     1681 ggtgcgcaca tcttcagtgc ctaccacgcg gctttctatt gttttccagc tattaaaata
     1741 gtccagcgcc tgcttggcat atttacccac tggcacgcta gttgggtccc cggtgcacag
     1801 tttaccgtta aaagccttgg ccaggtcaac gcctttgctc agggttacct gtgggctggc
     1861 atttaaagga gctatgagga caagctggtt ggcgagcaaa ttggtgcggc tagatttatc
     1921 gatgaggttg cgcttttcta aataatcggc ccagtcttta tcggctgaaa taaatacatc
     1981 ggcattggcg ccattttcga tttgtttcgc gagggttgcg gagcttgcga aggagttttt
     2041 aatgctggag gcaggggttg atttttgata cgcctgggcg agttcgctta acgcatcggt
     2101 gaggcttgcc gctgcataga ctttcacttc cgctgcatat gcaccaggtg gcagcaaaaa
     2161 acagttaaca gcgatcaata ccgaagcata aagttggcag cggagcctgt tacggttcgg
     2221 gtggggctgt tttaatagag gaaacataga ctactccgat aattgtatac ctattgggtg
     2281 attcgttatg catgattgta tatcacgccg atggtgtcaa tattaaatgc cagcttgcag
     2341 tatttatttt tggtgatgct ttaacctggt ttgaatttgc atgggatgtg catttcaaca
     2401 tgtgcgttta tattgctccc cggcagctag gttgcgcagg gacgccacaa aattgatgta
     2461 attgggtcgt gaggcgatac tatattcggc ctatacagga tattcggtaa gggggtataa
     2521 gcgtgtattg attggcgcgt attaaagaat aacaatcgcc ttcaagtgag attcacaact
     2581 gggtgaaggt tacattgtgc tatgccataa ggaagcatct gatagattgc ttggctcgct
     2641 tttgcggcgt tctaattaac aaataaaggc gtgttaaagg aagaaagtac ctgtttttaa
     2701 aatgcaattc gccagtgtat tacaggctaa atctcggtag ttactatccc cgcggcaaga
     2761 tagttcgtcc tgatttattt tgccttcgct gcgctaaacc tggggaaaat gcatggattt
     2821 tcttgtttta acgcagctcg cggcggtttg caccgccgag cgcggcatcc ttgccgcgag
     2881 tattaacccg gcactaaatg ccgccgggtt gatactcaca ttgcggatgg atgtgtgaat
     2941 gtaactcctg tggttgcccc cttgcgcctg ggttatatat tggtatgtgt ggcgaggagg
     3001 gcacccacaa ggggtgcccc tacggctgct tgctgctcag tatatcctta gctttatgac
     3061 atgggtttat tgtatggtgc tttttgctga gcgattgtaa cttacattct gcttacaatt
     3121 taccgcttgt ggcgaggttg agctcgcgga tctcatcacc ctcggttatt ctggcaaata
     3181 taagtattcc atcttgggaa tattcatact cgtgctgcac atccacctcg ttataaacaa
     3241 ttttttggca gaggagcatt ttgccttctt catcgtaggt ggctttaaaa taggtgttgc
     3301 ggttgtgcat ttgctcgggg ctgatttcat taaccaggtt taatggcaac ttcatgccgc
     3361 tgtagctaat aaaaaagcga atgttgggtg tgtggttcat ggcgtattta agggtttggc
     3421 aagtttgttg agtaaataaa ccttttcatc aagggcgcag ggaccgatgc tggggtgctc
     3481 tgggtctatg cgcacgcaac ttgctccgtg caataattta atttgatatt ccttttggta
     3541 cagcgcactg acttcgctat cgatgcgttg tgccgagggc agagcatcaa gggcggttag
     3601 attggtttcg ctggattcca ccgtcattaa taatattggg ctgctagaag gcaaggtgcg
     3661 ccttaaatga gccacataca aaatacgcag ctgcggcggc agggcggtaa gagcggcgca
     3721 gtcgtagact aatttaattt tattaaggtc tcttagacgc aaatcaaaaa tatcgccgca
     3781 gagaatttgc cgctgttcaa attgccaacg aataaaattg ccctgcacct gctgtttggg
     3841 tgtgatggcc agggtggcaa aataggcggc gatggcaaca gcgcttactt caacgcctat
     3901 tacacttaaa ccctgttgta taaaccaatc catatccaca cttttaccgc acaggggcac
     3961 cagcacttga tcgccagggc acagttttaa actgggcaaa tactgttgta ataatgggtt
     4021 tacctggggc tggtgaaagg caatatcatt gcgccgccac ttatcttgcc aggtgttttg
     4081 caagtgatgt tgccatttat cctgggtttt actgcgagaa ttttttgcgc tcatgactga
     4141 tactctggcg atgaaccagg aatcagttca aagtggttta tttaaagcct gggatggggg
     4201 cttgttattt cgcgttttat gttgtcttgg ggttaagaat cgagcctgta ttcgcctgcg
     4261 tcgatcatgc cttgtaatat ggcctgcaag cgttttggac tgatgaggct gccgggttct
     4321 tctgcgcgcg gcatcacatc taaaagaata ttctgtgcgt gcaattgctc gattatttcc
     4381 tccataaaat attctaacga acaggccgcc ggtgcgccgt cgtgccaata atcaagggcc
     4441 gcttcggtgg cgaatatttt atagggccag agtggaaaat aatgttcgcc gttgatcgtg
     4501 cgggtgaaaa agcggttggc gctacttagg taccatactt cttccgcctc aaatacgcgg
     4561 gtcataaaat attccaggcg ctcggtatcg gtcatggaat taagggccgc taactcgcag
     4621 actaatgggc tatggggcat aggttactcc aagcataagc aacttgttga aatactacct
     4681 tgctagctat ggcggcgtta aattttggca aaaagtcctc atttactcgt tgtaaactcg
     4741 gcaattgctc ctgcattgct ctacctcctg catccatgca gtcgt
//
LOCUS       DAOQGS010000313         4784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9351_length_4784_cov_5.927046, whole genome shotgun sequence.
ACCESSION   DAOQGS010000313 DAOQGS010000000
VERSION     DAOQGS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9351_length_4784_cov_5.927046"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..849)
                     /locus_tag="PKZ52_11860"
     CDS             complement(166..849)
                     /locus_tag="PKZ52_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNG60510.1"
                     /translation="MLIVIEALLSREEVNLARAQLLNASWVDGKVTAGTQSAAVKTNR
                     QLAENSPVAQQLGELILRKLGANPLFLSAAVPDKIFPPLFNRYGVGEGFGLHIDNAIR
                     PIKGTSQAIRTDLSATLFFSDASDYDGGELHIETSYGAQSVKLNAGDLVLYPARSLHR
                     VTQVTRGERLASFFWLQSMVRDDAQRSLLFDLDQSIQAVAQELGANHGEVLRLTGVYH
                     NLLRTWASC"
     gene            complement(862..1365)
                     /locus_tag="PKZ52_11865"
     CDS             complement(862..1365)
                     /locus_tag="PKZ52_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNG60511.1"
                     /translation="MAIKPWPLPIRLIHWLMVGLITADLLFLETGERAHKFVGFTLAG
                     LVCIRLALLGLSRNPAYKINLPKLAAIKQQITQRQWAYTHHSPLGALMICTIWGLLLA
                     AATSGYLQTTDALWGEEWVEQLHSVIVYSLFTAIALHVSAIALLQRRAKLPLVQRMWR
                     GVPDKNP"
     gene            complement(1355..1621)
                     /locus_tag="PKZ52_11870"
     CDS             complement(1355..1621)
                     /locus_tag="PKZ52_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNG60512.1"
                     /translation="MFVLTAASAKTFADADCPKAPQDKWLPVYEMQKKIINDYKFAIK
                     KFATTGNCYEIYGWALNATGTQWEKIEVYFDPVTGAIVKKVNGD"
     gene            complement(2024..4321)
                     /locus_tag="PKZ52_11875"
     CDS             complement(2024..4321)
                     /locus_tag="PKZ52_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNG60513.1"
                     /translation="MSSPVLRPLNLRQLLSAPVFVLGAGVSHSYAAEPTATSRLEKLT
                     VEEAVVAPNANPRAQEGAPYKVNELASPLYTRPIADTPKTLQVISKEAMQDAGVQDLA
                     DVMRAQPGITLGTGEGGNAFGDRFIIRGFEARNDVFVDGVRDPGVTTRETFAVEQVEI
                     AKGPNASFAGRGTTGGAINNVTKKAGASDFTDVDLGLGTDAKKRVTVDNNAVLTDTLA
                     LRSNLMYGERDLPARGLASEQRQGAAFAGAWKASEQFSLLADYYYQASDDVPDGGIAW
                     DPTTGAPIKGRDYFGQIGRDFWRTSSNIGTLVAKWDISNTLRWQTTLRDGKTTNRYVI
                     SISGLAANPSPGAGVPVGAQTPGVFVRANSQNRNQTNDAQTLQSILNWDIAPGGMQHS
                     LVFGIEAAEEDITNLPYSDSLRGANAGTPGNPNNFFWRQNGGKLTVNTAGYSTLTVES
                     RSVFLMDTWTLSEQWELFAGARADQFDFSVNSGATAYTGATNGAKTYDESMGNGHLGL
                     VYKPTDYGNIYLNFSTSSNPTGEQLDSATNCAYGGLCQNGNNAGAASGFTPTPEKNTN
                     YELGSKWELFDDALLLSGAVFEIIKEDVISTTNVPNSNPAITLVNQVGELQVRGFELA
                     ATGNINERLSVQTGITWLDTEITQSDTPSEIGQKFPNTAEKSANLQLRYQLLNKLALG
                     GLVTYKGEIWGGTPNGGTTNNKIDANTRLDLMADWEISQQFSLQFNLQNATDEVYYDA
                     LYRSATPFTYVGEGRSLTVTAKMAF"
     gene            complement(4350..>4784)
                     /locus_tag="PKZ52_11880"
     CDS             complement(4350..>4784)
                     /locus_tag="PKZ52_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG60514.1"
                     /translation="EPSLPFMQPIAVTAPQFVVQAAAITPPSQVAPAAVQPLAAPAAP
                     AAPQADANPYYQMVLRQIAQHKRYPAQAKRIRQQGLVEISFEISADGRLLSAQVSQSS
                     QVPSLDAAALAAVTNASPFPPIPAELGVSRLRLSLPIEFSLR"
BASE COUNT          987 a         1218 c         1419 g         1160 t
ORIGIN      
        1 acatccgtgt aggctcgtca aaaaggtccc tctttttgac ggtctgcact aacctgtagt
       61 caccactgct acaccgcgaa tcgattatgc cggatggcta aatcctcctg aatttgccgc
      121 cagcgcggaa gaagggagta aagtgtgctg aatagttacg atgagtcaac aactggccca
      181 ggtgcgcaat aaattgtggt acacaccagt aaggcgcaat acttccccgt ggttggcgcc
      241 caattcctgg gctaccgctt ggatgctctg gtctaaatca aataataaac tgcgctgggc
      301 gtcgtcgcgc accatgcttt gcaaccaaaa aaatgaagct aaacgctcac cgcgggtgac
      361 ctgggtcact cggtgtaaac tgcgggcggg gtagagcact agatcgccgg catttaattt
      421 cacactttgg gcgccgtagc tggtttcaat gtgcaactca ccgccgtcgt aatcgctggc
      481 gtcgctaaaa aataaagtgg cggataaatc ggtgcggatg gcttggctgg tacctttgat
      541 cggccgaata gcgttgtcga tatgcaggcc aaaaccttca cccacgccgt agcggttaaa
      601 tagcggtgga aatattttat cgggcacggc ggcggataaa aacagcggat tggcgccgag
      661 tttgcgcaga attaattcgc cgagttgctg ggcaaccggg ctgttttccg ctaattgccg
      721 attggttttg actgcagccg attgtgtgcc cgcagtcact ttgccgtcca cccagctggc
      781 atttaataat tgcgctcgcg ccaggtttac ttcttcgcgg cttaacagcg cttcaataac
      841 aattaacatg agcctggcct tttatggatt tttatcgggt acgccgcgcc acatgcgctg
      901 taccaggggc aatttagcac ggcgttgcaa gagtgcaatg gcacttacat gcagggcgat
      961 ggcggtaaat aaactgtaca caataacgct gtgcagctgc tccacccact cctcacccca
     1021 gagggcatcg gtagtttgta aataaccact ggttgcggcg gctaataata aaccccaaat
     1081 ggtgcaaatc atcagtgcgc ccaaggggct gtggtgtgta taagcccact ggcgctgggt
     1141 tatttgctgt ttgatggcag cgagtttggg cagatttatt ttataggccg ggttgcggct
     1201 caaccccaac agcgccagac gaatacagac taagcctgcc agcgtgaagc cgacgaattt
     1261 atgggcccgc tcgccggttt ctaaaaacaa caggtcggcg gtgataaggc ccaccatcaa
     1321 ccagtgaatt aagcggatgg gcaggggcca gggtttaatc gccattgact tttttaacta
     1381 tagcgccggt aactgggtca aaatacactt caattttttc ccattgggtg ccggtggcat
     1441 taagtgccca gccataaatt tcgtaacaat taccggtggt ggcaaatttt ttaatggcga
     1501 atttgtagtc gttgataatt tttttctgca tttcatacac cggcaaccat ttatcttgcg
     1561 gcgctttggg gcagtcggcg tcggcgaagg ttttggcact ggcggcagtt aaaacgaaca
     1621 taacaagcct ggcgagtttt aacataatga atagtctcgt gttggttgat ggggtgtgag
     1681 ctgcatttat taccgagtta cccgcaatga atacacatca tttcagacac gccgtaaata
     1741 catccttctt gtaggctcgt caaaaaggtc cttttttttg gcggtctgaa actatgtgtt
     1801 ctccttgctt ccgtacagtt aatattaacc cggcggcatt tagcgccggg ttaatactgg
     1861 cggcatggat gccgccccgg cggtgcaaac cgccgcgagc tgcactaaaa caagaaaatc
     1921 cgcgcatttt cgcccgtttt agtgcagcaa aggcaaaata gatcaggacg atctattttg
     1981 ccgccgggtt aaaaagtgcc gctcacacca ttttcgctgg aaattaaaac gccattttgg
     2041 cggtaaccgt taagctgcgc ccctcgccta cataagtaaa cggcgttgcc gagcggtaga
     2101 gggcgtcgta gtaaacttcg tcggtggcat tttgcaggtt aaattgcagg ctaaattgtt
     2161 ggcttatttc ccaatcggcc attaaatcca aacgggtatt tgcgtcgatt ttattattgg
     2221 tggtgccgcc gttcggtgtg ccgccccaaa tttcaccttt gtaggtaact aggccaccta
     2281 aggctaattt attcaataat tgatagcgca attgcaggtt cgcgctcttc tcggcggtgt
     2341 tggggaattt ttgcccgatt tcactcgggg tatccgattg cgtaatttcg gtatccaacc
     2401 aggtgatgcc cgtttgtacg gataaacgct cgttgatatt accggtggcc gctaattcaa
     2461 acccgcgtac ttgtaattca cccacttggt tcaccagggt aatggccggg ttgctgttgg
     2521 gcacattggt ggtggagatt acatcctctt taataatttc aaataccgcg ccgctgagca
     2581 gtagggcatc gtcgaataat tcccatttgc tgcccagctc gtagttggtg tttttctccg
     2641 gcgtgggggt aaagccgctg gcggcacctg cgttgttgcc gttttggcag agaccgccgt
     2701 aggcgcagtt ggtggccgaa tctaattgtt ccccggtggg gttggaggag gtgctgaaat
     2761 ttaaatagat attgccgtag tcagtgggct tgtacaccag tcccaaatgg ccgttgccca
     2821 tggattcatc ataggttttg gcgccgttgg tggcacctgt gtaggcggtg gcgccggagt
     2881 tgacagagaa atcaaactga tcggcgcggg ccccggcgaa taattcccac tgctcgctta
     2941 aggtccaggt atccattaaa aatacactgc ggctctccac tgtgagggtg gagtagccgg
     3001 cggtattcac cgttaacttg ccgccgtttt gccgccagaa gaagttattg gggttgccgg
     3061 gggtgcccgc attggcgccg cgcaggctgt cgctgtaggg caggttggtg atgtcctctt
     3121 cggccgcctc aatgccaaac accaaggagt gctgcatgcc gccgggggct atgtcccagt
     3181 tgaggatgct ctgcagggtt tgtgcgtcat tcgtttggtt gcggttttgc gagttggccc
     3241 ttacgaacac gccgggggtt tgcgccccca caggcacacc ggctccgggg gatgggttgg
     3301 cggccaggcc ggatatggag attacatagc ggttggtggt tttgccatcg cgcagggtgg
     3361 tctgccagcg cagggtgttg ctgatatccc acttggcgac caaggtgcct atgttgctgg
     3421 aggtgcgcca aaagtcccgg ccgatctggc cgaagtaatc gcggcctttg atgggggcgc
     3481 cggtggtggg gtcccaggcg ataccgccat cgggcacatc atcgctggct tggtagtaat
     3541 aatcggccaa caggctgaac tgctcgctcg ccttccaggc accggcaaag gcggccccct
     3601 ggcgctgttc gctggctagc cctcgggcgg gcagatcgcg ctcgccgtac atcaggttgc
     3661 tgcgcagggc gagtgtatcg gtgagcaccg cgttgttatc caccgtgaca cgttttttcg
     3721 cgtctgtgcc caggcccaga tccacatcgg taaagtcgct ggcgcccgct tttttggtga
     3781 cgttattgat cgcgccgccg gtggtgccgc ggcctgcaaa gctggcgttg gggcccttgg
     3841 caatttccac ctgctccacg gcaaaggtct ctcgtgtggt gacgcctggg tcccgcacgc
     3901 cgtccacaaa cacatcgttg cgggcctcaa agccgcgaat aataaagcga tcgccaaagg
     3961 cattgccgcc ttcacccgtg cccagggtaa tgcccggctg ggcgcgcatt acatcggcca
     4021 ggtcttgcac ccctgcgtcc tgcatggctt ctttggagat cacctgcagg gttttggggg
     4081 tatcggcgat ggggcgggta tagaggggcg aggccagttc gttcactttg tagggggcgc
     4141 cttcttgagc gcgggggttg gcgttgggag ccactaccgc ctcctccaca gtgagttttt
     4201 ccagccttga ggttgcggta ggctctgcgg catagctgtg gctgacgcca gcgcctagga
     4261 caaaaacggg tgcgctcagc agctggcgca ggttgagggg gcgtaaaaca ggcgaagaca
     4321 tagggtttag ttccaaagta ggtaaataat taacgcaggg aaaattcaat cggcaggctc
     4381 agccttaaac ggctcacgcc cagctctgca ggtatggggg gaaaggggct ggcattggtg
     4441 acggcggcta aggcggccgc atccaagctg ggcacctggc tggattggct cacctgggcg
     4501 gataacaagc ggccatcggc gctgatttca aaactgatct ccaccaaacc ctgctggcga
     4561 atgcgcttgg cctgggcggg gtagcgtttg tgctgggcga tttggcgcag caccatttgg
     4621 taataggggt tggcatcggc ctgtggggcg gcgggggctg ccggtgcggc cagcggttgc
     4681 actgctgcgg gagccacttg gctgggcggc gtaatggccg cggcctgcac cacaaattgc
     4741 ggggcggtaa cggcaatggg ctgcataaag ggcaggctgg gctc
//
LOCUS       DAOQGS010000314         4772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9384_length_4772_cov_5.091160, whole genome shotgun sequence.
ACCESSION   DAOQGS010000314 DAOQGS010000000
VERSION     DAOQGS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_4772_cov_5.091160"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKZ52_11885"
     CDS             complement(<1..744)
                     /locus_tag="PKZ52_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNG60515.1"
                     /translation="MCQLLGMSCQSPASIDFSLQGFTARGGMTDEHKDGWGIGFFRQA
                     RWQVFTDANPAAHSPLTSQLQQEKIKSETVLAHIRKATVGEVNERNCHPFVRTIAGQS
                     WVFAHNGHLPDYAPALNGDYVPRGDTDSEKAFCWLLQEVQARLSSDQLQAAVPTSLFA
                     LLHQLCQEIAQAGVFNILLSNGNWLFGFCSTNLSYLQRQYPFAEARLVDLDVTLDLNA
                     VNSQDDRMVLLSTKPLTHNEPWVSLNKGEA"
     gene            1109..1612
                     /locus_tag="PKZ52_11890"
     CDS             1109..1612
                     /locus_tag="PKZ52_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG60516.1"
                     /translation="MSVVYLNSAKSSDAASEIPDSELDLALQTAQAEAAASNLGYAGR
                     VSPEDAWRLFTSGKAHIVDVRAIEERKFVGHVPNTLHVAWQTGPALIKNPRFLRELEQ
                     KLAKDVVVLFLCRSGKRSAAAAEAASKAGFTHAFNIIEGFEGDLDGNQQRGSNGWRAK
                     NLPWVQD"
     gene            1710..2690
                     /locus_tag="PKZ52_11895"
     CDS             1710..2690
                     /locus_tag="PKZ52_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNG60517.1"
                     /translation="MSVKAPDQDVQKGQWPIDQIVQELREARSQWRNLAGRSQELGQR
                     DLPSRQALEIIFEQLCGALFPLRLGPPDLRAKTEDFYVGHTLDLALNELWQQVLLELR
                     FYHRNSTQHRNTDQIALNAELKQNASNIVHQFAQKLPEIRRLLDRDVQAAFNGDPAAR
                     SFDEVLLCYPGITAIIHHRLAHELYRLDLPLLARILAELAHSRTGIDIHPGAQIGPGF
                     FIDHGTGVVIGETAIIGARVRLYQAVTLGAKRFPVDAQGSLEKGLPRHPIVEDDVVIY
                     AGATILGRITIGKGSVIGGNVWLTRNVAAGSQVTQANSQQLLCESTDEPI"
     gene            2677..3012
                     /locus_tag="PKZ52_11900"
     CDS             2677..3012
                     /locus_tag="PKZ52_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG60518.1"
                     /translation="MNRSNEPITQAFGQKIRIIRKSFGWSQEALAHRAGLNRSYLGQI
                     ERGETLVSIVTIEKLAKAFRLSLSLLLAELPTPAPSTRTESTEQSTTVPNTPAYSIRY
                     LSAAEPNTK"
     gene            3308..4243
                     /locus_tag="PKZ52_11905"
     CDS             3308..4243
                     /locus_tag="PKZ52_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNG60519.1"
                     /translation="MSNEQDLQLALGDHAARQLANATKTTPQLSTISPRWLVHMLQWL
                     PVEAGIFRLNKVKNPTRVTVTCSGRDEAEITPTFVDYEENPREYFLNAVTSVVDVHTR
                     VSDLYNSPHDQIKEQLRLTVETIKERQESELINNKEYGLLANVDDSQRISTLTGAPTP
                     DDLDELITKVWKEPGFFLTHPLAIAAFGRECTRRGVPPPTVSLFGSQFLTWRGIPIIP
                     SEKVPVDENGKTKILLLRVGEKRQGVVGLYQPGLAGEQSPGLAIRFMGIGRNAIASYL
                     ISLYCSLAVLTEDALAVLDDVDVSKYHDYPDTYKA"
     gene            4215..>4772
                     /locus_tag="PKZ52_11910"
     CDS             4215..>4772
                     /locus_tag="PKZ52_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60520.1"
                     /translation="MTTQIPTKPNLPLDIADLTALANAFFHALPGESVDLALPVNTQA
                     AQAAQLSVPSTAQLATQVPAQKHQAQLPVEPFSFYGDISPQAFGLPTEAQLRALLMDG
                     PQYGQGLIAPAASAPVSSSAVHLPRASQDPFLHPSARPQHTPGNYYFLSESGPWSRPQ
                     EHTTPVKTQLDVNAIRKDFPILAERV"
BASE COUNT         1294 a         1252 c         1099 g         1127 t
ORIGIN      
        1 cgcttcaccc ttattaaggc tcacccaggg ttcgttatga gttaaaggtt tggtggataa
       61 caacaccata cgatcatcct gggaattaac cgcatttaaa tccaaggtca catccaggtc
      121 caccagccga gcctcggcaa acggatactg ccgctgcaaa taacttaaat tggtcgagca
      181 aaaaccaaat aaccaattgc cgttggacaa taaaatatta aacacacccg cctgggcaat
      241 ttcctggcag agctggtgca gcagcgcaaa taaagaggta ggcaccgccg cctgtaattg
      301 atcagaactc aagcgcgctt gcacctcctg cagcagccaa caaaaagcct tttcactatc
      361 ggtgtcgcca cgggggacat aatcaccatt gagtgccggc gcatagtccg gcaggtgccc
      421 attgtgggca aacacccagg attgcccggc tatggtgcgc acaaatggat ggcagttgcg
      481 ctcgttgacc tccccaactg tggctttgcg aatatgggcc agcaccgtct ccgatttaat
      541 tttctcctgc tgcaactgac tggtgagcgg cgaatgggcg gctgggttgg catcggtaaa
      601 cacctgccag cgggcctggc gaaaaaaccc tatgccccag ccatctttat gctcatccgt
      661 catgccgccc cgcgcggtaa aaccctgtaa agagaaatca atactggcgg gactctggca
      721 actcatacct aacaactgac acataaccaa cgcttccaaa aactcaactg atcctgagga
      781 atactatttc agcatagata agaaataact gtgccaactg aaattcaacc aggggcgcag
      841 cgtttaaata gcgctgatcg gcatacagcc cgctgtcacc aggggcaaat ctcggcccct
      901 aatattggcc aaaaactatg tttacccgaa ccacaaatgc gcccaggtgc accgataaca
      961 acaggccagc tgatgaaatc tacacagtga aatttttata taagaatatg tctttatatt
     1021 atttcccttg cgtttaggcc ctgactatat ttcgttgcaa taactcacac ccatttaatc
     1081 acacgcgagt aatcgccgag gaaaacatat gtcagtggtg tatctaaact ccgccaaatc
     1141 atcggatgcg gcaagcgaaa ttcctgattc agaactagac cttgcactgc aaactgccca
     1201 agcagaagcc gccgcaagca atctcggcta cgccgggcgc gtatcgccgg aggatgcctg
     1261 gcgtttattt accagcggta aagcccatat cgtagatgtg cgcgcaattg aagaacgcaa
     1321 atttgttggc catgtgccaa acacattaca tgtcgcctgg caaactggtc ccgccctaat
     1381 aaaaaacccg cgttttttac gcgagctaga gcagaaatta gcaaaagatg tggtggtgtt
     1441 atttttgtgc cgcagcggca agcgttctgc agcagcggca gaagcagcta gcaaagcggg
     1501 ttttacccat gcgtttaata ttattgaagg atttgaaggt gatttggacg gcaaccaaca
     1561 gcgcggctcc aacggctggc gggcgaaaaa tttaccctgg gtgcaagact aatatcgctg
     1621 cgtaaaaaaa tcgatgtaca gttgataccg cactgaattt tcgtcgcggc aggcaataga
     1681 taattaataa caatgaaatt gagataccca tgagtgttaa ggcacctgat caggacgtac
     1741 aaaaaggcca atggcctatt gatcaaatag tgcaagaact gcgcgaagcc cgcagccagt
     1801 ggcgcaattt agcggggcgt tcacaggaac taggccaacg ggatttaccc tcgcgccaag
     1861 cgctggaaat aatttttgag caactttgcg gcgcgctttt cccgctgcgt ttaggcccac
     1921 ccgatttgcg cgctaaaact gaagattttt atgtgggcca cacactggat ttagccctca
     1981 atgaattgtg gcagcaagta ttgttagagc tgcgttttta ccaccgcaat agcacacaac
     2041 accgcaatac cgatcaaatt gccctcaacg cagaattaaa acaaaacgcc agcaatatag
     2101 tgcatcagtt tgcacaaaaa ttaccggaga ttcgccgcct gttggatagg gatgtgcaag
     2161 cggcttttaa tggcgaccca gccgcgcgct catttgatga ggtattactc tgctacccag
     2221 gcattacggc cattattcac caccgcttag cccatgaatt ataccgcttg gatttaccgc
     2281 tgctggcgcg cattttggcg gaattagctc actcccgcac cggtattgat atacatcccg
     2341 gtgcacagat tggcccaggt ttttttattg atcacggcac aggtgtggta ataggtgaaa
     2401 cagccattat cggcgcccgt gtgcgcttgt accaagcggt taccttaggc gctaaacgct
     2461 ttcctgttga cgctcaaggc agtttagaaa aaggcctgcc acggcatccg attgtggaag
     2521 acgacgtagt gatctatgcc ggagcaacaa tcttaggccg tattaccatt ggtaaaggat
     2581 ccgtcattgg cggcaatgtg tggttgacgc gcaacgtggc agcgggcagc caagtaaccc
     2641 aagccaatag ccaacaactg ctgtgcgaat ccaccgatga accgatctaa cgaaccgatt
     2701 acccaggcgt tcgggcaaaa aattcgcatt attcgcaaat cctttggttg gtcccaagag
     2761 gcattagcgc accgagcggg tttaaaccgt tcgtaccttg ggcaaattga acgcggcgaa
     2821 acccttgtct caatcgttac tattgaaaaa ttagccaagg catttcgctt atccttaagc
     2881 ctgttattgg ctgaattgcc aaccccagcg cccagcactc gaacagaaag cactgaacaa
     2941 agcaccacag tgcccaacac accggcatat agcattcgtt atctatctgc cgcagaaccc
     3001 aatacaaaat aaatgtttaa atatcaacac ttactgttta aaaatcagaa aaaaattgtt
     3061 tttcctgagc ttttgatgcc tataggcggt tgagccttgc gcaggctctg ccggattata
     3121 gcgccgcgcc gacgaggcct gcgtgtctat tttgtgcgcc cacactgctc agcagataac
     3181 tgcagctaat tttagtaatc gcgttgtaaa aacacatcta tttatttgcc agcaggttat
     3241 ttattacccg caggcaatca aattgttata caccatttca taagtttgcc atcattggaa
     3301 taccgctatg agtaatgagc aagatctaca gctagccctt ggcgatcacg ccgcgcggca
     3361 actggctaat gccaccaaaa ccaccccgca actctccact atttcacctc gctggttagt
     3421 gcacatgctg caatggctgc cggtggaagc cggtattttc cgcttaaata aggttaaaaa
     3481 cccaactcgt gtaacagtga cctgctcagg ccgcgatgaa gctgaaatca cacctacctt
     3541 cgtggattac gaagaaaatc cccgtgaata ttttcttaat gcggtgacca gcgtagtcga
     3601 tgtgcataca cgtgtatccg atttatacaa cagcccccac gaccaaatta aagagcagct
     3661 gcgcttaacg gttgaaacca tcaaagagcg gcaggaaagt gaattaatta ataataaaga
     3721 atacggcttg ctcgccaatg tggacgacag ccaacgcatc agcactctca ccggcgcacc
     3781 gacacctgat gacctggatg aattaattac caaagtctgg aaagaaccgg ggttctttct
     3841 cactcatccg ctggccatag ctgcgtttgg ccgcgaatgt acccgccgcg gtgtgccgcc
     3901 gccaacggtg agtttattcg gttcacaatt tttaacctgg cgcggcattc cgattattcc
     3961 atcggaaaaa gtacccgtag atgaaaatgg taaaactaaa attttattgc tgcgtgtagg
     4021 cgaaaaacgt caaggcgtgg tggggttata tcagcccggt ttagccggtg aacaaagccc
     4081 aggccttgct atccgcttta tgggcattgg ccgcaacgcc atcgcctcct acttaatttc
     4141 tctctattgc tctttagcgg tattaactga agatgctttg gcggttttag acgacgtgga
     4201 tgtaagcaag taccatgact acccagatac ctacaaagcc taatttacct ttggatattg
     4261 ccgacctcac tgccttggcc aatgcatttt ttcatgcact gcccggtgaa agtgtagatc
     4321 ttgcgctgcc tgtgaatact caagcagcgc aggctgcaca attatcagtg cccagcactg
     4381 cgcaactggc aacacaggtt cctgcacaaa aacatcaagc gcaattgcct gtcgaaccat
     4441 ttagctttta cggcgatata tcgcctcaag catttggttt acccaccgaa gcgcaattgc
     4501 gcgcgctgct tatggacggg ccccagtacg gccaaggatt aattgcacca gccgcttcgg
     4561 cacccgtctc atcaagcgcg gtacatttac cgcgcgccag ccaagatccg tttttacatc
     4621 ctagtgcgcg acctcaacac acgccgggca attattattt tttatcggaa agcggtcctt
     4681 ggtcgcgtcc ccaggaacat acaacgccgg ttaaaaccca attggatgtt aacgccattc
     4741 gcaaggattt tcccatattg gctgagcggg tc
//
LOCUS       DAOQGS010000315         4766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9403_length_4766_cov_3.946084, whole genome shotgun sequence.
ACCESSION   DAOQGS010000315 DAOQGS010000000
VERSION     DAOQGS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9403_length_4766_cov_3.946084"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..2857
                     /locus_tag="PKZ52_11915"
     CDS             182..2857
                     /locus_tag="PKZ52_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNG60521.1"
                     /translation="MPQSLIPALIRRVSWLTLGSASLFSHLAVAEEPPQKPQLPYQNL
                     ALTPVERAKDLVSRMSWEEKIPQLINDAPAIPRLGVREFNWWNEGLHGVAALGEATVF
                     PQAIGMAAAWDAPLLKQAAGVVSTEFRAKHYAKQHRFGGSDWFAGLTVWAPNINIFRD
                     PRWGRGQETYGEDPHLTARLGVAYVQGLQGTDPKYLKTVATPKHYAVHSGPEATRHKD
                     NVNPSPKDLEETYLPAFKATVQEGQAGSVMCAYNAINGVPACANQALIKDTLRGKWGF
                     EGFVVSDCAAVYDIYSPKNHAYTPTPEAGIKAAFDAGMDLICGDADEADHIRVALQKG
                     LISQQQIDTALIRLFTARMRLGQFDPRPQVFPEITASANDTPDNRKLAQTLAEKSIVL
                     LKNQNNFLPLGASIKTLAVIGPNADSTEALVGNYNGSPSHPVTLLTGLQKRFGTGAVR
                     YAQGTGLLYPLEAPVPDSVLCVDAKCKKPGLKAEYFADKSFADKTLATKPLKQQVEPN
                     VQVSWKDSVRSTAMRWSGFIKAPETGRYTLRYKADGGYRIWVNDQLIIDAWKLDWRAV
                     IASGSIELNAGQIYSLKVESFQRGEQGNEQLIWSIPSDPGAQAALAAANAADAIIFAG
                     GLTAQVEGEEMPVPMPGFVGGDRTDLALPAGQRALLEKLYATGKPIVVVLMNGSALAV
                     NEEDAKATALLEAWYPGGQGGDAIAAILAGDVSPAGRLPVTFYKSLEQLPAFNDYSMS
                     NRTYRYHKSPVLYPFGYGLSYSAFRFGEIKVQPKQWKPGNTLRLSVNITNTGGRDADE
                     VVQLYLARPDIPGSPIRSLAGFQRIKIAKGATENVIFELQAEQLSTVDETGKRAVVAG
                     KADIWIGGGQPDARPGLPITAGSRASVQITSGQDL"
     gene            complement(3161..4126)
                     /locus_tag="PKZ52_11920"
     CDS             complement(3161..4126)
                     /locus_tag="PKZ52_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNG60522.1"
                     /translation="MPQILSIGEVMVELSPAGSHQNKRLMALGFAGDTYNTAVYLARL
                     GLEVGFFTRLGDDPYSAEAIATMAAEGLATSGVEVIPGRTAGLYMIANQANGERNFTF
                     WRSQSPARDLFSSQRSLATFKQHLQQTQQVYFSGITLAILSEAARVEFFSLLQGFRAK
                     GGRVVFDNNYRQALWPDQNSAQIAMQQALECADSALLTDDDYERLWGSAEFAQVLQRC
                     RNAGVHEVVLKRGSDPVCVAWRVGQGELLTAEVAVPVVNDVIDTTAAGDSFNAGYLTA
                     RAQGQTPVKAAQFGSRCAAIVIRHRGAIVEREVFLAEFMQNQYYV"
     gene            4351..>4766
                     /locus_tag="PKZ52_11925"
     CDS             4351..>4766
                     /locus_tag="PKZ52_11925"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNG60523.1"
                     /translation="MNRSAAQPLVLNPDRLFSSDPAQRAIARRLYTSIKNLPIISPHG
                     HTDPSWFADNEPFGNASELLIRPDHYVFRMLYSQGISLEALGIPSKDGAAVETDARKI
                     WHLLAHHYYLFRGTPSRLWLDHVFQEVFGLEVSLSPA"
BASE COUNT         1199 a         1407 c         1168 g          992 t
ORIGIN      
        1 cgccaaattt tggcaaaaaa gcgcccatat ccagcgtctt aggcaccctc gctacaagtt
       61 caactgcggt ttttaggtta taaacaaata aataggcgcc gcacctggtc tgtatcctca
      121 ggtttcatca aaaaaccacc tcaaaaccac ctcaaaacca cctcaaaata agagattaac
      181 catgccacaa tcgcttatcc ccgcgctgat acgccgtgtt agctggctaa cactgggttc
      241 tgcaagcttg tttagccacc ttgcagtggc cgaagagccg ccccaaaaac cccaattgcc
      301 ctatcaaaac cttgcactta cccccgttga gcgcgctaaa gatttggtga gccgcatgag
      361 ttgggaggaa aaaatcccgc aattaattaa cgacgcaccg gccattccgc gcctcggcgt
      421 gcgtgagttc aactggtgga acgaaggcct acacggggtt gccgccctgg gggaagctac
      481 tgtattcccc caagccatcg gcatggcggc cgcctgggat gcaccgctgc taaaacaagc
      541 ggctggtgtg gtgagcaccg aatttcgcgc caaacactat gccaagcaac accgctttgg
      601 cggctcggat tggtttgcag gccttaccgt ttgggcaccg aacattaata tctttcgcga
      661 cccgcgctgg ggccggggcc aggaaaccta tggagaagac ccccatttaa ctgcccgtct
      721 aggcgtcgcc tatgtacaag gcctgcaggg tactgatcct aaatacttaa aaaccgtcgc
      781 caccccaaaa cactatgccg tacacagcgg ccccgaggcg acccgccaca aggataacgt
      841 caaccccagc cccaaagact tagaagaaac ctacctgccc gcctttaagg ccaccgtgca
      901 ggagggccaa gcaggctcag tgatgtgcgc ctataacgcc atcaacggcg tgcccgcctg
      961 cgccaaccaa gcactcatta aagatacctt gcggggcaaa tggggctttg agggttttgt
     1021 ggtgtccgac tgcgctgccg tatacgacat ctacagcccc aaaaaccacg cctatacccc
     1081 aacgccagaa gcgggcatca aagccgcctt tgatgccggt atggatctca tttgcggcga
     1141 tgcggatgaa gcggaccaca tccgcgtggc cctgcaaaaa ggcctcatca gccaacagca
     1201 aatagacacc gcgctgattc gcctctttac cgcccgcatg cgcttaggcc agttcgaccc
     1261 gcgcccgcag gtattccccg agattaccgc cagtgccaac gacacccccg acaatcgcaa
     1321 actggcgcaa accctggcgg aaaaatccat cgttctgctg aagaaccaga acaacttttt
     1381 acccctcggc gccagcatca aaaccctcgc cgtgatcggc cccaatgccg atagcaccga
     1441 ggccctggtg ggcaactaca acggttcgcc ctcccacccg gtaaccttac tgacaggcct
     1501 gcaaaaacgc tttggcacag gagccgtgcg ctacgcccag ggcaccggcc tgctctaccc
     1561 cttggaagct ccagtgcctg atagcgtttt gtgtgtggat gccaagtgca aaaaacccgg
     1621 ccttaaagcg gaatactttg cggataaaag ttttgccgat aaaaccctcg ccaccaagcc
     1681 ccttaaacag caggtagagc cgaacgtaca ggtgagctgg aaagacagcg tgcgctcaac
     1741 agccatgcgc tggagcggtt ttatcaaagc gccggagacg ggccgctaca cattgcgcta
     1801 caaggccgat ggcggttacc gcatttgggt taacgaccag ctgatcatcg acgcttggaa
     1861 actcgactgg cgggcggtga ttgccagcgg cagcatcgag cttaacgccg ggcagatcta
     1921 cagcctgaag gttgaaagct tccagcgcgg tgaacagggc aacgagcagc tcatttggag
     1981 cattccatct gacccaggcg cccaagctgc cctggccgct gcgaatgcag ccgatgccat
     2041 catttttgca ggcggcctaa ccgcccaggt ggaaggcgaa gaaatgcccg tgcccatgcc
     2101 cggctttgtc ggcggcgacc gcacggatct tgccctaccc gcaggccagc gcgccctatt
     2161 ggaaaaactc tatgccaccg gtaaacccat agtagtggta ctgatgaacg gcagcgccct
     2221 tgcggttaac gaagaggacg ctaaagccac cgcattgctt gaagcctggt accccggcgg
     2281 ccaaggcggc gacgccattg ccgccatact cgcaggcgat gtcagccccg ccgggcgcct
     2341 accggtcacc ttctataaat ccctcgagca gttgcccgcc tttaacgatt actccatgag
     2401 taaccgcacc taccgctacc acaaaagccc agtcctctac cccttcggct acggcctctc
     2461 ctacagcgcc ttccgcttcg gggaaattaa agtgcaaccc aaacaatgga agcccggtaa
     2521 caccctacgg ttaagcgtga acatcaccaa taccggcgga cgggatgccg acgaagtggt
     2581 gcagctgtac ttggcgcggc cagacatccc aggcagcccc atccgcagcc tcgcaggctt
     2641 ccagcgcata aaaatcgcca aaggcgccac ggagaatgtg atctttgaac tgcaggccga
     2701 gcaattaagc actgtggatg agactggcaa gcgggcagtg gtggcaggca aggcggatat
     2761 ctggatcggc ggcggccaac ccgacgcccg cccaggccta cccatcaccg ccggtagccg
     2821 ggctagcgtg cagatcacct cggggcagga tctttagagc aggatgggtg aattggcaag
     2881 gaacaagcag ggacgctttt ttgcctgcct ttatttctag gctgatttag cgaaaggaac
     2941 acattaaaca caagaattga ttaaacgaag taaagcaatt aaacgaaagg aacttaatta
     3001 aataccaaac tgattcccca gtagcgctga aagtgtatta gtcacgatct aatctgacac
     3061 ttcggcttga aataagagag ttaccggttt tgaatatggg tgtcgaatcc taaccaaaac
     3121 acaaaggtaa ctctcatgct acaaactaca aatctcatca ttaaacataa tattggtttt
     3181 gcataaactc ggctaaaaaa acctcccgct caactatagc gccgcgatgt ctaattacga
     3241 tagcggcgca gcggctgcca aattgtgccg cctttacagg cgtttgcccc tgagcacggg
     3301 cagttaaata acccgcatta aaactatcac cggctgcagt ggtgtcaata acatcattta
     3361 ccacaggcac agccacttct gcagtcaaca attccccttg cccgacccgc caagcaacac
     3421 acaccggatc agagccgcgt tttaatacca cctcatgaac acccgcattg cggcagcgct
     3481 gtaaaacctg cgcaaattca gcactgcccc ataggcgctc gtaatcatca tcggtgagca
     3541 aagcgctatc ggcgcactca agtgcctgct gcatggctat ttgcgctgag ttttgatcgg
     3601 gccagagggc ttggcgataa ttattgtcga acaccacccg accgcccttt gcgcgaaatc
     3661 cttgcaataa actaaaaaat tctacccgtg ccgcttcgct taaaatagcg agggtaatac
     3721 cgctgaaata aacctgctgg gtttgctgca ggtgctgttt aaatgtggcc aaggaacgct
     3781 ggctggaaaa taaatcccgc gccggtgatt ggctgcgcca aaacgtaaaa ttgcgctccc
     3841 cattggcttg gttggcgatc atatacaggc ccgctgtacg gccgggaatt acttcaaccc
     3901 cactcgtagc taggccttcg gcagccatgg tcgctatagc ttcagcgctg tacgggtcgt
     3961 cacctaagcg cgtgaaaaaa cccacctcaa ggcctaagcg cgctaaataa actgccgtgt
     4021 tataggtgtc acccgcaaaa cccaacgcca ttaaacgctt attttggtgg ctgccggcgg
     4081 gggataattc caccattact tcaccgattg aaagaatctg cggcatagtt ttctctttaa
     4141 gtaaattgtc gaaccaatat aggtggcagg taatttcgtt gcattatccc tattctagcc
     4201 ctgtttatgc ctactctaaa cttagggtgc cgtaataata gtttttaaag cagttttgcc
     4261 tgttggctat ttagcgccct gagtaactgg gttattatcc ggccttagtt tcttcacgtt
     4321 taaattcgcc cgcgcacaag gtgatacgcc atgaaccgct ctgcagcaca accgctggta
     4381 ttaaatcctg accgattatt ctcctctgac ccggcgcagc gcgctatagc ccgccgactt
     4441 tacaccagta taaaaaattt acccatcata agcccccacg gccacacgga ccccagctgg
     4501 tttgccgata acgaaccgtt tggcaacgcc agtgaattat taatccggcc cgaccattat
     4561 gtatttcgga tgctctacag ccaaggaatt tcccttgagg ctctgggcat tcccagcaag
     4621 gatggtgcgg cggttgaaac ggatgcgcgc aaaatttggc atctgttagc gcaccattat
     4681 tatttatttc gcggcacccc gtcgcggctg tggttggatc atgtatttca ggaagtattt
     4741 ggcttagagg taagtttatc gccagc
//
LOCUS       DAOQGS010000316         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9404_length_4765_cov_5.317197, whole genome shotgun sequence.
ACCESSION   DAOQGS010000316 DAOQGS010000000
VERSION     DAOQGS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9404_length_4765_cov_5.317197"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKZ52_11930"
     CDS             complement(<1..265)
                     /locus_tag="PKZ52_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60524.1"
                     /translation="MHSSTLSFDEAYSAATPQVRPRAYGKAGLSPVAKLNCLALVCLL
                     KVGLGVILLTHWNKGDEQRLETVLTVRTLADDTAPLPLAAPPEP"
     gene            complement(409..1041)
                     /locus_tag="PKZ52_11935"
     CDS             complement(409..1041)
                     /locus_tag="PKZ52_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60525.1"
                     /translation="MGAKTCMLIYQDEGAKGALAEYPPLDRAATDRLVQALFPAANPI
                     GTGSLANTHPPKHSLYAGCFGGLSIVAAREFGDDRPSRLPQRFIDYGKNQHIYLHAMH
                     SVVDWFAFGHWQGGVLVRALSLSPDRGLIEDLGSKEPFEQGFWHGERPATAGADEDYP
                     LAFHPLDLAEEALQAILGFQLEGPLNPDALQPERIPLVVYSRAKPWWQFW"
     gene            1208..1591
                     /locus_tag="PKZ52_11940"
     CDS             1208..1591
                     /locus_tag="PKZ52_11940"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HNG60526.1"
                     /translation="MKLPILTLLCSLLLSPVALALRCGSDVIDEGMRKIEVEQKCGPP
                     ASKDSRIEYRTVRKHRSVRDDYSGYNDLYELRQRRDRQSEEEVETVQVVIEEWVYNFG
                     STRFMQLLIFEDGRLKEIKDMGYGK"
     gene            complement(1962..2225)
                     /locus_tag="PKZ52_11945"
     CDS             complement(1962..2225)
                     /locus_tag="PKZ52_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNG60527.1"
                     /translation="MTWKLLFTKQAQKDAQKLSAAGLKNKAQELLQIIEANPYQNPPP
                     YEKLVGDLAGAYSRRINIQHRLVYQVLSEEKIIKVLRLWTHYE"
     gene            complement(2222..2464)
                     /locus_tag="PKZ52_11950"
     CDS             complement(2222..2464)
                     /locus_tag="PKZ52_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009722422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNG60528.1"
                     /translation="MQTLSASEARANLYRLLDETAQSHQPIGIQGKRNNAVLVSAEDW
                     EAIQETLYLLSVPGMRESIKEGMAEDPSDCAEELDW"
     gene            2925..4394
                     /gene="nifE"
                     /locus_tag="PKZ52_11955"
     CDS             2925..4394
                     /gene="nifE"
                     /locus_tag="PKZ52_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HNG60529.1"
                     /translation="MKAKDIAQLLDEPACEHNKKEKSGCAKPKPGATDGGCAFDGAQI
                     ALLPIADVAHVVHGPIACAGSSWDNRGTRSSGPDLYRIGMTTDLTEQDVVMGQSEKRL
                     FHAIKQAIEDHQPPAVFVYNTCVPALIGDDVDAICREAQARWNVPVVPVDAAGFYGTK
                     NLGNRIAGDAMVRYVIGSREPDPLPAGTERPGIKVHDVNLVGEYNIAGEFWHVLPLLD
                     ELGLRVLCTLSGDARFREVQTMHRAEVNMMVCSKAMLNVARKLQEKFGTPWFEGSFYG
                     ITDTSQSLRDFARLINDPDLTARTEALIEREETRIRALLEPWRERLNGKRVLLYTGGV
                     KSWSVISALQDLGMKVVATGTKKSTEEDKQRIRELMGDDVKMLDEGNPKLLLQTVADY
                     KADILIAGGRNMYTALKARIPFLDINQEREFGYAGYEGMLELVRQLCLTLESPVWAAV
                     RKSAPWGSSPGLALSQFEQGQIDQNQFDQDHHEQGVAAL"
     gene            4391..>4765
                     /locus_tag="PKZ52_11960"
     CDS             4391..>4765
                     /locus_tag="PKZ52_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNG60530.1"
                     /translation="MTEIIKRKKALSVSPLKTSQTSGGALAFLGVARSVPLMHGSQGC
                     TAFAKVFFVRHFREPVPLQTTAMDQVSSVMGADDNIVEALKVICEKNNPALVGLVTTG
                     LAETQGADINRAVGEFRHRFPQY"
BASE COUNT         1012 a         1275 c         1336 g         1142 t
ORIGIN      
        1 tgggctctgg cggcgctgca aggggtaggg gcgctgtatc atcagccagg gtgcgcacgg
       61 ttaatacggt ttctaagcgc tgctcatcgc ccttgttcca gtgcgtcaga agtattaccc
      121 ccaggcccac tttcagcaga cacaccaggg ctaaacaatt gagcttcgcc accggcgaca
      181 ggcccgcctt gccataggcg cgggggcgca cctgaggtgt ggcggctgag taggcttcat
      241 cgaagctaag cgttgagctg tgcataggtg tgtaccggtt tgaatacaag cacatcctat
      301 ttgataattg ttatcatctg caagcaattg ctgaaagttt tttatttgta tttggctgca
      361 tgtcctcgca gctcgacgac tagggcgccg gaaccaggcg caaggggatt accagaattg
      421 ccaccagggt ttggcgcggc tgtacaccac cagggggatt cgctctggct gcagggcgtc
      481 ggggttaagc ggcccctcca actgaaaccc caaaatggcc tgaagggcct cttcagctag
      541 atccagaggg tgaaaggcta aggggtagtc ctcatccgcc cctgcagtgg cgggccgttc
      601 cccgtgccaa aacccctgtt caaatggctc tttagaacct aggtcttcga taaggccccg
      661 gtcgggcgaa aggctcaggg cgcgcaccag cacaccgccc tgccaatgcc caaacgcaaa
      721 ccaatccacc acgctgtgca tggcgtgtag gtagatatgc tggtttttac cgtagtcgat
      781 aaaccgctgc ggcaaacgcg aagggcgatc gtcgccgaat tctctggcgg caacaatgct
      841 caggccgccg aaacagcccg cgtaaaggct gtgtttgggc gggtgggtat tggccaggct
      901 gccggtgcct atggggttgg cggctggaaa gagcgcctgg acaagccggt cagtggccgc
      961 gcgatcaaga ggcggatact cggcaagagc gcccttggca ccctcgtctt ggtatatcag
     1021 catgcaggtt ttagcgccca taatgaggct ccatggaagt ggtgggtagt gaggattatg
     1081 ctgcgtttta gtcggtattt attaccgctt gggcctgttt gccacaaaag gcgcgctccg
     1141 acaaatcagc ttgctagact cttgctgtac catggccgct cgcctgcctt attaccaagg
     1201 tcgccttatg aagttaccta ttctcaccct gctgtgctcc ctattgttaa gccctgttgc
     1261 cctagcactg cgctgcggct cggatgtgat cgacgagggc atgcgcaaga ttgaagtcga
     1321 acaaaaatgc ggccccccgg cctccaaaga ttcccgtatt gaataccgca ccgtgcgcaa
     1381 acaccgctcc gtgcgcgacg attacagcgg ctataacgac ctatacgaac tgcgccagcg
     1441 ccgcgaccgg cagagtgaag aggaagtgga aacggtgcag gtggtgattg aagagtgggt
     1501 ttataacttt ggctctaccc ggtttatgca gttgttgatt tttgaagatg ggcggttgaa
     1561 ggaaattaag gatatggggt atggcaaata ataatacgct tggttaggaa tttattgcgt
     1621 gaagcgtttt tttaaccttt atccctattg gtcgggtttt gccgtttggg ttttaggagt
     1681 tgcgtgtcat ggattgttat atgtgtactc gactttgcat gggccgccaa ttcctgacgc
     1741 ttacgccaac gagatatggt ttcaattgat ggcctttttt atcgtccgct tcccctattg
     1801 gttcgcaaca ttaatacttt tgatcctgtg tgttcgattc tacaaagcta aaaagtctgt
     1861 aaaaatcgct gcacaataaa tttcgttaat ttcttgataa ttcttttttg cgcaagcact
     1921 ttagggtctg ataattgggg cttcgcgcgt gcctgctggt tttattcgta gtgcgtccaa
     1981 agtcttagca ctttgataat tttttcttcg ctcaacacct gataaacgag cctgtgttga
     2041 atgttaatgc ggcgggaata ggcaccggcc aaatcgccca ctaatttttc atagggtggg
     2101 ggattttgat atgggttggc ttcaattatt tgcagtaatt cctgagcctt attctttagg
     2161 ccagcggctg agagtttttg cgcgtctttt tgcgcctgtt ttgtaaacag tagcttccat
     2221 gtcaccagtc caactcctca gcgcaatcgc tgggatcttc ggccatgccc tctttgattg
     2281 attcgcgcat accgggaact gagagcaggt agagagtctc ttgaatagct tcccaatcct
     2341 cagccgaaac gagcacagcg ttgttgcgtt tgccctgtat cccaatgggc tggtgggact
     2401 gggctgtttc gtcgagcaaa cggtacaaat ttgcccgcgc ttcactggct gataatgttt
     2461 gcatagtaaa ttcctcagta tgagttttaa gcgtacgtca ttacgtacgt atcggcaagt
     2521 gctggtcttt caaggtaaat ggcaagttgc gggctgtgaa ttgcacacct tttaacctcg
     2581 gacactctgc gtaggggcaa ccattgtggt tgcccgttgt gcctatcttc aactgtgtcc
     2641 gggcgcgggc ggccacaagg gccgccccta cagtcgcgcg cgaaatcgca cttgtaacac
     2701 ctgtcgcacc tacgccaatc ctaccggcca ccgccttgtc gcagatcgtg caaatcccgc
     2761 cccttgtagg ttttgcgaca gcgacttatc cccactaagt ccttaaaaat cagccgctta
     2821 agcgctggtc tggcatttgc acccctccat ttaaccaaac ccctccacac cagagcgcca
     2881 ctgcctgtgt ggccccctta attttgtttg cgaaaggtct gcctatgaaa gctaaggaca
     2941 tcgcccagct gcttgatgaa cccgcctgcg aacacaataa aaaggagaag tccggctgcg
     3001 ctaagcctaa gcctggcgcc accgatggcg gttgtgcctt tgatggcgcg cagattgcgc
     3061 tgctgcccat cgccgatgtg gcccatgtgg tacacgggcc aattgcctgt gcgggcagtt
     3121 cttgggataa ccgcggcacc cgctcctcgg ggccggatct ctaccgtatc ggcatgacca
     3181 ccgatcttac cgagcaagat gtggtgatgg gccagagtga aaaacgcctc tttcacgcca
     3241 ttaaacaagc cattgaagat caccagccgc cagcggtgtt tgtgtacaac acttgtgtgc
     3301 ccgccctgat tggcgatgat gtagacgcta tctgccggga ggcccaggcc cgttggaatg
     3361 tgccggtggt gccggtggat gccgctggtt tctacggcac taaaaattta ggcaaccgca
     3421 tcgcaggcga tgccatggtg cgttatgtga tcggcagccg ggagccagac ccactgcctg
     3481 cgggcacgga gcgcccgggc atcaaggtgc acgatgtgaa cctggtaggg gaatacaaca
     3541 ttgccgggga gttctggcat gtgttgcccc tgttggatga gttgggtctg cgagtgctct
     3601 gcaccctctc cggcgacgcc cgttttcgcg aagtgcaaac tatgcacagg gcggaggtga
     3661 acatgatggt gtgctccaaa gccatgttga acgtcgcccg taaactgcag gaaaaattcg
     3721 gcaccccttg gtttgaaggc agcttttacg gcattactga tacctcccaa tccctgcggg
     3781 attttgcccg cttgattaac gaccctgacc tcaccgcccg cactgaagct cttatcgagc
     3841 gggaggaaac ccgcattcgc gcgctgcttg aaccctggcg tgagcgcctg aacggcaagc
     3901 gggttctttt atataccggt ggagttaagt cttggtctgt catctccgcc ctgcaggatt
     3961 tgggcatgaa ggtggtggcc acgggcacta aaaagtccac cgaagaggac aagcagcgca
     4021 tccgtgagct gatgggggat gatgtgaaaa tgctcgatga gggcaacccc aagctgctgc
     4081 tgcaaaccgt ggccgattac aaagccgata tccttatcgc gggcggccgc aacatgtata
     4141 cggccctcaa ggcgcgcatt ccctttttgg atattaacca ggagcgggaa tttggctatg
     4201 caggttatga aggcatgttg gagctagtgc gccagctgtg tttgaccctt gaaagcccgg
     4261 tgtgggcagc ggtgcgcaaa agcgccccct ggggatcaag cccaggcctt gccctcagcc
     4321 aattcgagca gggccagatt gatcaaaacc agtttgatca ggatcaccat gagcagggag
     4381 tggccgccct atgactgaga tcatcaaacg caaaaaggcc ctctcggtca gccccttaaa
     4441 aaccagccaa acctccggcg gcgccttggc ctttttgggg gtggcccgct cggtgcccct
     4501 gatgcacggc tcccagggct gcacggcgtt tgccaaggta ttctttgtgc gccactttcg
     4561 cgagccggtg cccctgcaaa ccaccgctat ggatcaggtg agttcggtca tgggtgctga
     4621 tgacaacata gtggaagcct taaaagtcat ctgtgaaaaa aacaacccgg ccctggtggg
     4681 gctggtcact actggccttg ccgagaccca aggggcggac attaaccgcg cagtgggcga
     4741 gtttcgccac cgcttccccc aatac
//
LOCUS       DAOQGS010000317         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9461_length_4746_cov_4.797058, whole genome shotgun sequence.
ACCESSION   DAOQGS010000317 DAOQGS010000000
VERSION     DAOQGS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9461_length_4746_cov_4.797058"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKZ52_11965"
     CDS             complement(<1..564)
                     /locus_tag="PKZ52_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG60531.1"
                     /translation="MSIEVRNLQKNFGQFTALNNVSLDFPTGELVALLGPSGCGKTTL
                     LRIIAGLENPDSGSVLFHGEDATDVHVRERNVGFVFQHYALFRHMTVFDNVAFGLTVR
                     PKATRPSKEEIKRRTHELLTLVQLDWLADRYPHQLSGGQRQRIAIARALAVEPKVLLL
                     DEPFGALDAKVRKDLRRWLRRLHDELHI"
     gene            complement(588..1481)
                     /gene="cysW"
                     /locus_tag="PKZ52_11970"
     CDS             complement(588..1481)
                     /gene="cysW"
                     /locus_tag="PKZ52_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNG60532.1"
                     /translation="MHSVAASLHPRSTKPSHGATTEAPWIRYSLIAVALSFLAFFLIV
                     PLMVVFTEAFSKGAGVYFAAITEPAAAHAIKLTLIAAGIAVPLNVVFGLAASWAIAKF
                     QFRGKQLAITLIDLPFAVSPVIAGLIYMLLFGAQGLMGGWLAEHDIRIMFAIPGIVLA
                     TIFITFPFIARELIPLMQEQGKEQEEAAITLGANGWKMFFRVTLPSIKWGLLYGIILC
                     NARAMGEFGAVSVVSGKIRGHTNTMPLHIEILYNEYQFAAAFAVASLLALLALVTLVL
                     KSALEWKAAREHELAIRTAQE"
     gene            complement(1484..2383)
                     /gene="cysT"
                     /locus_tag="PKZ52_11975"
     CDS             complement(1484..2383)
                     /gene="cysT"
                     /locus_tag="PKZ52_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNG60533.1"
                     /translation="MSFAPSASSPPGQTISTPKTASKKQPWVQKSVLPGFGLSLGFAT
                     LYASLLVFIPLAGLLIKSLELPLDQLWDVISNRRALASYRLTFGASLIAATLNLFFGL
                     IVAWVLARYNFPGKRIVDAMVDLPFALPTAVAGIALTAIYAPNGIIGQWFALLDIKIA
                     YTWIGVTVALTFIGLPFVVRTVQPVLVDLETEAEEAAASLGATRWQTFRYIIIPALLP
                     SLLTGFALSFARAIGEYGSVVFISGNMPYRTEITPLLIMTKLEQFDYAGAAALGSIML
                     VISFAILLLINGLQHWSHKRAGG"
     gene            complement(2494..3486)
                     /locus_tag="PKZ52_11980"
     CDS             complement(2494..3486)
                     /locus_tag="PKZ52_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNG60534.1"
                     /translation="MRLLTQIFLLAVTGFAASAGFAKEVKLLNVSYDPTRELYREYNA
                     AFAKYWLGKTGDTVTVEQSHGGSGAQSRAVIDGLQADVVTLALAGDIDPLAKIGKLLP
                     EDWQNRLGDNSAPYTSTIVFLVRKGNPKHIKDWDDLIKKGVEVITPNPKTSGGARWNY
                     LAAWGFAKKKLGSDEAAQDFVKKLYKNVPVLDSGARGATTTFVQRQIGDVLLTWENEA
                     YLAVNELGADKVEIVVPSISIVAEPSVSLVDKNAEAHGTKEVAQEYLKFLYSKEAQNI
                     IAKNYYRPRDKEVAAAYASKFPSIELITIDHFGGWKKAQPEHFGDGGIFDKIYQ"
     gene            complement(3573..>4746)
                     /locus_tag="PKZ52_11985"
     CDS             complement(3573..>4746)
                     /locus_tag="PKZ52_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNG60535.1"
                     /translation="LIALSSQQAAAGFPTLYGKILLTANSYDLEKNNFALTAAGATTY
                     KHTGATGVATELDSVAVESTGSRLGVQGDFGVAHDLTVFYRIEYGIDVDNGTNSNGRE
                     LSQRNVFAGIRSADWGQVLVGKNDTPLKTLQTTSVLRTDLDRFNDYPLADIGTYLAGE
                     NRPDNVIQYTSPIILGGLEVNLAAIQAEETGVAVNATNQQNDNGFATGKSLSVVYGKA
                     KWYVGAAYETNVATADTLRLAGEVTIGPVKLGGFYQTAERHEDYDTIGGLTSTFVGSA
                     VNSQGVQNGLNPLAEWDGAAGTAYKEQDAVVLNGQWKIAGPWVAKVQVAQATSTPSAA
                     FADVDTDAAAIGVDYNLSEAARIFSYYASLKTRGDQKINTVASVDKTYAVGLDLRF"
BASE COUNT         1275 a         1184 c         1131 g         1156 t
ORIGIN      
        1 gatatgtaat tcatcgtgca acctgcgcaa ccagcggcgc aaatctttgc gcaccttagc
       61 atctagggcg ccaaagggtt catccaataa taaaaccttt ggctccaccg ctagcgcgcg
      121 ggcgatggcg atacgctggc gctggccgcc ggataattgg tgggggtagc gatcggccaa
      181 ccaatccaat tgcaccagtg tcaataattc atgggtgcgg cgtttaattt cttctttgct
      241 cggccgcgtg gctttggggc gcacggttaa accgaaggcc acattgtcaa ataccgtcat
      301 gtggcgaaat aacgcgtagt gctgaaatac aaaccccaca ttgcgctcgc gcacatgtac
      361 atcggtggca tcctcaccgt gaaataatac gctgccgcta tcgggatttt ctaacccggc
      421 aataatacgc aacagcgttg ttttgccaca cccggaaggg ccgagcaatg caactaactc
      481 accggtggga aaatccagag agacattatt taacgccgta aattggccaa aatttttctg
      541 taaattgcga acttcgatac tcataaaaaa ttcttactct agggcaatta ttcttgggcg
      601 gtgcggatag ctaattcgtg ttcccgcgca gccttccact ccagcgcact tttgagcacc
      661 agggtcacta aggccaacag cgccagcaaa gaggcaaccg caaaggccgc cgcaaattga
      721 tactcgttgt ataaaatttc gatatgcagc ggcatagtat tggtatggcc gcgaattttg
      781 ccgctcacca cactcaccgc accaaactca cccatggcgc gggcgttaca taaaataatg
      841 ccgtacaaca aaccccattt aatggaaggt agggttacgc gaaaaaacat cttccaacca
      901 ttagcgccca gggtaatagc cgcctcttct tgctctttgc cctgctcttg cattaaagga
      961 attaactcgc gggcaataaa agggaaggta ataaatatag tggctaaaac tatgccgggt
     1021 atagcaaaca taatgcgaat atcatgctcc gctaaccagc cgcccattaa cccctgggcg
     1081 ccaaataaca gcatataaat taaaccggca ataaccgggg agacggcaaa gggtaaatcg
     1141 atcagagtaa tcgccagttg tttgccgcga aattgaaatt tggcaatcgc ccaactggcg
     1201 gccaaaccaa ataccacatt gagcggcacg gcgatgcctg cggcaatcag agttaattta
     1261 atggcgtggg ccgcagcagg ctcggtaatc gctgcaaaat acaccccggc gcccttgcta
     1321 aaggcctcgg taaacaccac cattaagggc acaattaaaa aaaacgctaa aaaactcagg
     1381 gcaacggcaa ttaaactgta gcggatccag ggcgcctcgg tggtggcgcc gtggctaggt
     1441 ttagtactgc gtgggtgcag gcttgcggca acactatgca tatttaaccc cctgcccttt
     1501 tatggctcca gtgctgcagc ccattaatta ataataaaat ggcaaaagaa ataaccagca
     1561 taatgctacc tagtgcagct gcacctgcat aatcaaattg ttcgagtttg gtcataatca
     1621 gtagaggagt aatttccgtg cggtagggca tattgccgct aataaacacc accgagccgt
     1681 attcaccaat ggcgcgggca aaggacagtg caaagccggt taatagcgag ggtaaaagag
     1741 cagggataat aatgtagcga aaggtttgcc agcgcgtggc gcctaaactg gccgccgctt
     1801 cttccgcttc ggtttctaaa tcgactaata ccggttgcac cgtgcgcacc acaaacggca
     1861 aaccaataaa ggttaaggct acggttacac cgatccaggt ataggcaatt tttatatcta
     1921 ataatgcaaa ccactgaccg ataataccat tgggtgcata aatagcggtg agcgcaatac
     1981 cggccacggc ggtaggtaat gcaaagggta aatccaccat agcatccaca atgcgtttgc
     2041 ctggaaagtt atagcgggcc aatacccaag ccactataag accaaaaaat aaatttaagg
     2101 tggcggcaat taaagaggcg ccaaaggtta agcggtaact agccagtgcc cggcgattac
     2161 tgatcacatc ccacaactga tccagcggca actctaggct ttttatcagt aaaccggcca
     2221 agggaataaa cactaaaagg cttgcgtata aagtggcaaa acccaaagac aaaccaaacc
     2281 ccggcagtac cgatttttgt acccagggtt gctttttgct tgctgtttta ggtgtggata
     2341 tggtctgccc aggtgggctt gacgctgatg gggcaaaact catttaaact atctctattg
     2401 gtgcaacacg cggcggttaa ctcgtataaa tgcgacaggc acattcgtgg caataaaaaa
     2461 cgcttaaccc tagggttaag cgttttttat tttttattgg tagattttat cgaaaatacc
     2521 accatcgcca aagtgttccg gctgggcctt tttccatcca ccgaaatgat cgatggtgat
     2581 cagctcaatg ctaggaaatt tactggcgta ggccgcggcc acttctttat cgcggggccg
     2641 gtaataattt ttagcgatga tattttgcgc ttctttgctg tagaggaatt ttaaatactc
     2701 ttgggccact tctttggtgc catgggcttc ggcattttta tccacaaggc ttacggaggg
     2761 ctcagcgaca attgagatgg acggcacaac aatttcaacc ttatccgcgc ccagttcatt
     2821 caccgctaaa taagcttcgt tttcccaggt taacaacaca tcaccgattt gacgttgtac
     2881 aaaggtagtg gtggcgccgc gagcaccgga gtcaagcacg ggcacatttt tgtagagttt
     2941 tttaacaaag tcttgcgccg cttcatcgga gcctagtttc tttttcgcaa agccccaagc
     3001 cgctaaataa ttccaacggg caccgccgga agttttgggg ttaggggtaa taacctcaac
     3061 gcctttttta atcaggtcat cccaatcttt gatgtgtttg gggttgccct tgcgcactaa
     3121 aaatacaatg gtagaggtat aaggcgctga gttatcgccc agtcggtttt gccaatcttc
     3181 tggcaataat ttgcctattt tagccagcgg atcaatgtca ccggccaggg caagcgtcac
     3241 tacatccgct tgtaaaccat caatcaccgc ccgcgattgg gcgccggagc cgccgtggga
     3301 ctgctcaacg gtaaccgtat caccggtttt acccaaccaa tatttagcaa acgctgcgtt
     3361 atattcgcgg tacaactcac gggtggggtc gtaggagacg tttaataact tcacctcttt
     3421 agcaaacccc gctgaggcgg caaatcccgt tactgccagt aaaaaaatct gtgttaataa
     3481 gcgcataaaa atttcaacct aaaagctaag gaatctctga aaaaaagaca ggtgccctta
     3541 aatgagcacc tgccaatggc gaatgtcagt gattaaaaac gtaaatccag gcccactgcg
     3601 taggttttat ccacgctcgc taccgtatta attttttgat ccccacgggt tttaaggctg
     3661 gcgtaatagc tgaaaatacg cgccgcttcg ctgaggttgt aatccactcc gattgcagcc
     3721 gcatctgtgt ctacatcggc aaacgccgcg ctgggtgtgc tggtggcttg ggccacttgc
     3781 actttggcaa cccaagggcc ggctattttc cattggccgt tgagtacaac cgcgtcttgt
     3841 tctttatagg cggtgccagc agcgccatcc cattcagcca gtgggtttaa gccattttgc
     3901 acaccctgag aattcacagc tgaacccaca aaagtagagg tgaggccgcc gatggtgtcg
     3961 taatcttcgt ggcgctcggc ggtttgataa aaaccaccta acttaactgg gccaatagtg
     4021 acttcaccgg ccaaacgcaa agtatccgcc gtggccacat tggtttcgta tgcggcgccc
     4081 acgtaccatt tggctttgcc atacacaact gagagggatt taccggtggc aaaaccattg
     4141 tcgttttgct gattggtggc atttactgca acgccggttt cctcagcttg aatcgcggca
     4201 aggttaactt ctaaaccgcc taaaataatg ggcgaggtgt attgaataac gttgtcgggg
     4261 cggttttccc cggctaaata agtgcctata tcagcgagcg ggtaatcatt aaaacggtct
     4321 aaatcagtgc gcaaaacgct ggtagtctgc agtgttttta acggcgtatc gtttttaccg
     4381 actagcacct gcccccaatc ggcacttcta ataccggcaa atacgttgcg ctggcttaat
     4441 tcgcggccat tgctgttggt gccgttatcc acatctatac cgtattcaat gcggtaaaaa
     4501 acggttaaat cgtgggctac accaaaatca ccttgcacac ctaaacgcga acctgtgctc
     4561 tcaacggcaa ctgaatctaa ttcagtcgca acgccggttg cgccggtgtg tttataggtt
     4621 gtggcgcctg cggcggttaa agcaaaatta tttttttcca aatcataact attggcggtt
     4681 aacaatattt tgccatacag agtagggaaa cctgctgcgg cctgttgtga gctcagtgca
     4741 ataagg
//
LOCUS       DAOQGS010000318         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9462_length_4746_cov_4.420593, whole genome shotgun sequence.
ACCESSION   DAOQGS010000318 DAOQGS010000000
VERSION     DAOQGS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9462_length_4746_cov_4.420593"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..771
                     /locus_tag="PKZ52_11990"
     CDS             223..771
                     /locus_tag="PKZ52_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60536.1"
                     /translation="MKLAAIFILARAHHGLCALLQLTEGCHLQKWRLNQDRLNDWIFG
                     KIFMTTLNEYWIPADTELNYTAKILLSLPRQPKALEEIKPLMEAKLLMLMEQAEREEA
                     GAVREALELDDSSNSLTYGMEDLAAIAWTLVYEDTALNMLLPMLELEPSQTEPVEPEL
                     QQLVEKQTFFQVMEAVSTLANL"
     gene            855..>4746
                     /locus_tag="PKZ52_11995"
     CDS             855..>4746
                     /locus_tag="PKZ52_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HNG60537.1"
                     /translation="MAEATQRALRHIENEVGNIDEYVAKKLGYPSVEAAHKGLMALQV
                     DTVAAAIYNIEARDRGIIIADQTGVGKGRQAASLIRYAVRNGKTPVFITVKPNLFSDM
                     YGDLVDIGSGNIRPFITNADVGVEYNGETLFVGKGKAHQEKLRNIVDTGDLPPNYQAL
                     FTTYDQLRGDDNIRRNVISALKNPLFILDEAHNAAGERKKEGKFGNKKTATNTTAGFV
                     FDALAGNPVVYLSATYAKRPDNMPLYYRTDLLDAVDTPEELIDAVNSGGVPLQQVVAY
                     MLAANGQLFRRERSFAGIKMNFVRDTANVSEQERISDSVNRGLRNIVMANRMFVEWFK
                     ENGKGIAPPGGAAMMAGNRADAMAGSVNPFTSVVHNYIRQLLLGIKVKAAAEKAIALH
                     REGKKPVIALENTMGSFLAEYVEKHKLKVGDPVKFTYNDILKTALERSRRVSFKDSKG
                     KSVAVTVELGELDLLTRNVYTQAEAEINKIDLSSLPASPIDYMLNELKKAGIEVAEIT
                     GRGYFVDYSGSVPVLGQRSAKQIKDRRKTVDNFNNGNIDALILNAAGSTGLSIHASER
                     FKDQSPRHMIVAQPMLDINVLMQMLGRINRTGQVELPEFTFLSLDLPSEIRPTATTKA
                     KMAKLNANTSANDKSDTSFEAPDFMNKYGDQVVNEFLQDNPIIAQETGISGSEDSALP
                     DLALRFTGRLALLDAKKQRQAMDEILALYAAKIDYLNSVGMNDLVSQDLPLDAYILES
                     KIVHEGKDPNSVFAGHTTLHKVNAKYLGKPPTASEAAAALKKSLQGREPLEVANAIIA
                     EKEADSAYLDETIAYVKKAEDEAAKKLAALEAYREKEDADQAKLAKLADAHTAAEIKV
                     EAAQKSVEMVMQDKQEAVEAIRKGLKIGDRMVIDTGDESSVAVVVDIKNTHKKGSGNP
                     WAASKLKLTLMVNSGARQITVNYRQLKNGLFVNYLDGRGTDGLEEIFNPLIAEDRREI
                     RYIATGNLIAGAAKLRGKIVNFTDRAGLVHQGILLPRKYGKEGEFEQGDTVSNFVARD
                     PEAIAKFLQGEGAEIGVKSPDKAIKVSAGRDKSLVISLPLSNKNKTVFAVKLDRLLRD
                     IVGDFYSQGSAHVAKIPGGKVTEAIKRLIELTPLTIPSSMRKQWEAAGGNPAPEPVMS
                     FDGQNFQAGAAPGGQLTEAKVPDVPKPAPNLEPKEKEEETGANGRIIGDKFITDAPTT
                     IYTTKKGKDIEGVIAKGVSLEEIKRHVDPYAWPRNGGVFVRLKHVEREAGPKFSLGEN
                     GGLVSDEEVRAVINRVQSNFGAKLKITAVASAVEL"
BASE COUNT         1313 a         1083 c         1299 g         1051 t
ORIGIN      
        1 gaccgctgga gcgatttgga aaaccatcta ctgttgatta cgatggttat gcgttcaaaa
       61 agatcgacca tttaattgtt gccaaggcgc ttgagtcgat taaaaacggt gggcgcgcct
      121 ccataataat cgcagctaat cgcgaggctg gtgttattgg tagcggtgcc gatagggtat
      181 tcttcaattg gttgtacagt cactataatg tgattgatca ttttgaagtt agcggcgatc
      241 tttattctcg ccagggcgca tcatggcctg tgcgcgttat tacaattaac ggaagggtgc
      301 catcttcaaa aatggcgcct gaatcaggat cgattaaacg attggatttt tgggaagata
      361 tttatgacca cgttgaacga gtactggatt ccagccgata cggaactcaa ctacacagcg
      421 aaaatactgc tgagcctgcc gcggcaaccg aaggcgctag aggaaataaa acctctaatg
      481 gaggcaaagc tgctaatgct gatggagcag gcggaacgag aggaggccgg ggcggtacgc
      541 gaagcgctgg aactggacga cagcagcaac agcctgacct atggaatgga ggatctagca
      601 gcaatagcgt ggactctagt atacgaggac acggcattaa acatgctgct accaatgctg
      661 gaactggagc caagccagac ggaaccagtg gagccggaac ttcaacagct ggtggaaaag
      721 cagacgttct tccaagtaat ggaagcagtg tcaactctag caaacctgta gttacgggcg
      781 gtgcgtcaga gtttcaggcc gagtatgttg ccgcgagctc cgggtttaat gacagcgtat
      841 taactccaaa aaatatggcc gaagccacac agcgggcttt gcgccatatt gaaaacgaag
      901 tgggcaatat tgacgagtat gttgccaaaa agcttgggta tccatctgta gaggcggcgc
      961 acaaaggctt aatggcgctt caggttgata cggttgcggc ggccatttat aacattgagg
     1021 cgcgcgaccg cggcattatc attgccgacc aaaccggcgt tggtaagggg cggcaggcgg
     1081 cgtcattaat ccgctacgca gttcgcaacg gcaaaacccc tgtatttata acagtaaagc
     1141 ctaatttgtt ctcggatatg tacggggatt tggttgatat tggctcaggc aatattcgcc
     1201 cattcattac taatgctgat gttggtgttg agtacaacgg tgaaacccta tttgtgggca
     1261 agggtaaggc gcaccaggaa aaattaagaa acattgtcga taccggcgat cttcctccaa
     1321 attatcaagc gctattcacc acttacgacc aattgcgcgg ggatgacaat attcgccgca
     1381 atgtcattag cgcgctcaaa aatccgctat tcattctcga tgaggcacac aacgcggccg
     1441 gagagcggaa aaaagagggc aaatttggca acaaaaaaac cgccaccaat acgacagcgg
     1501 gatttgtttt tgatgccttg gcggggaacc ccgtagttta tttgtctgca acctacgcca
     1561 agcgcccaga caatatgccg ctgtattacc gcaccgacct attggacgcg gttgatacgc
     1621 ctgaagaact aattgacgcg gttaattccg gtggcgttcc gctgcagcag gtggttgcct
     1681 atatgttggc ggccaatgga cagctattta ggcgcgagcg ctcatttgcc ggtatcaaaa
     1741 tgaatttcgt gcgggacaca gcaaacgtgt ctgagcagga acgaatttca gattcggtga
     1801 accgcggatt gcgcaatatc gttatggcaa accgcatgtt tgttgagtgg tttaaggaaa
     1861 acggtaaggg gattgcgccg cctggcgggg ccgccatgat ggctggcaat agggcagacg
     1921 ccatggcagg tagcgtcaac ccctttacct ctgtggtaca caattacatt aggcagttgc
     1981 tgcttggaat taaggtgaag gctgcggccg aaaaggcaat tgcactgcac cgagagggca
     2041 aaaagcctgt tatcgccctg gaaaatacca tgggttcatt cctggctgaa tacgttgaga
     2101 aacataagct aaaggttggc gacccggtta aattcaccta caacgatatt ctaaaaaccg
     2161 ccctggagcg ctcgcgccgg gttagcttta aagactcgaa gggtaagagc gttgccgtca
     2221 ctgtagagct tggcgagctg gatttgctca cccgaaacgt atacacccag gcagaggccg
     2281 aaattaacaa aattgatttg agcagcctgc cagcatcgcc tattgattac atgctaaacg
     2341 agctgaaaaa ggccggaatc gaagtggctg aaattaccgg gcgcggctat ttcgttgatt
     2401 attctgggtc tgtcccggtg ctcgggcagc gcagcgccaa acaaattaag gaccggcgca
     2461 agaccgtgga taatttcaac aatggaaata ttgacgcgct gattctgaat gcggccggat
     2521 caaccgggtt aagcattcac gcgagcgagc gatttaagga ccagagcccg cgccatatga
     2581 ttgttgccca gccaatgctg gacattaacg tattaatgca aatgctcggc agaattaaca
     2641 gaaccggcca agttgagctg ccagaattta cattcctatc tttggacctg ccttcagaaa
     2701 ttcgcccaac ggccactacc aaggccaaga tggcaaaact aaacgccaac accagcgcca
     2761 atgataaaag cgatacctca tttgaggcgc ctgatttcat gaataaatac ggcgatcagg
     2821 tggtaaatga attcctgcag gataacccaa taatagctca agagaccgga atatcaggat
     2881 cggaagattc agcccttccc gatttggcgc tgcgctttac cgggcgtctg gcgttgctcg
     2941 atgccaagaa acagcgccag gccatggatg aaatattggc gctttacgct gccaaaattg
     3001 attacctcaa tagcgtgggc atgaatgatt tggtgtctca agatttgccc ctggatgcct
     3061 atattctcga aagcaaaatt gtgcacgaag gcaaagaccc aaatagcgtt tttgctgggc
     3121 acacaacttt gcacaaggtt aatgctaagt atctgggcaa gcccccaact gcaagcgagg
     3181 ctgccgcggc gctcaagaag tctttgcagg gtagagaacc gctagaagtg gcaaacgcca
     3241 ttattgccga gaaggaagcc gatagcgcct atttggatga gacaattgcc tacgtgaaaa
     3301 aagccgaaga cgaggccgcc aagaaattgg ccgcactgga ggcttatagg gaaaaagagg
     3361 acgcagacca agccaaatta gccaagctgg cagatgcgca tacggcggca gaaattaaag
     3421 ttgaggccgc ccaaaaatcc gttgaaatgg taatgcagga taagcaggag gctgtagagg
     3481 ctattcgcaa aggcttaaaa attggcgacc ggatggtaat tgatactggc gatgagtcgt
     3541 ctgttgctgt tgtggtggat attaaaaaca cccacaaaaa aggcagcggc aacccttggg
     3601 ctgcatctaa gctaaagcta accctaatgg tgaactcggg cgcgcgtcaa atcacggtta
     3661 attaccggca attgaaaaac gggctgtttg ttaattatct tgacggcagg ggtaccgatg
     3721 ggcttgagga aatatttaac cctctaatag ctgaggaccg tcgggagatt cgctatatcg
     3781 ccactggtaa cctaattgct ggggcggcaa agctgagggg caaaattgtt aatttcacgg
     3841 accgcgcagg ccttgtgcat caaggcattt tgttgccgcg caaatacggc aaagaaggcg
     3901 aatttgagca gggtgacact gtttcaaatt ttgtcgctcg cgatccggaa gccattgcaa
     3961 aattcctaca gggcgaaggc gcagagattg gtgttaagag tcccgataag gccataaagg
     4021 ttagcgctgg gcgcgacaaa agcctggtga tttccttgcc gctatccaat aaaaataaaa
     4081 ccgtgttcgc ggttaaattg gatcgcctat tgcgcgatat tgttggggat ttttatagcc
     4141 agggcagcgc ccatgtggcg aaaatccctg gcgggaaagt aaccgaggct attaagcggc
     4201 taatagagct cacgcctctt actattccct cctcgatgcg caagcagtgg gaagcggctg
     4261 gcggtaatcc tgcgccagaa ccggttatgt cttttgatgg gcaaaatttc caagccggag
     4321 ctgcgccagg cggacaattg acagaggcca aagtgcctga cgtacctaag ccagcaccga
     4381 atttggagcc aaaagaaaaa gaagaagaaa ccggggctaa cggccgcatt attggtgata
     4441 aatttatcac tgacgctcct accacgattt acacaaccaa gaaaggcaag gacatcgagg
     4501 gcgttattgc caagggtgtt agcctggagg aaattaaacg gcatgttgac ccttacgcat
     4561 ggccgcgcaa tggcggtgtg tttgtacgct taaagcatgt tgagcgcgag gcagggccaa
     4621 aattctccct gggcgaaaat ggcggcctag tcagcgatga agaggtgcgt gcggttatta
     4681 atcgagtgca gtccaacttt ggcgcgaaac tcaaaataac tgcagttgcg agcgcggttg
     4741 agctcc
//
LOCUS       DAOQGS010000319         4720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9539_length_4720_cov_4.203215, whole genome shotgun sequence.
ACCESSION   DAOQGS010000319 DAOQGS010000000
VERSION     DAOQGS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9539_length_4720_cov_4.203215"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKZ52_12000"
     CDS             complement(<1..652)
                     /locus_tag="PKZ52_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNG60538.1"
                     /translation="MRVPKLSSAVIFLAVFSLISGCSSKKEEKISSEQKLYEKAQLAL
                     NASNWDSAIKALQLIEEHYPFGSYGEQSQLEIIYAHYEADQFDEAIAAADRFIRLHPQ
                     HRNTDYAYYMRGMASFNKERSFLSSLLSIDNTNRDPGAAKDSLAQFTDFLTRYPKSPY
                     APDAQKRMVYLRNVLARYEIHVANYYFKRGAYLAAANRGRYVVENFQTTPAVPDALA"
     gene            764..1723
                     /gene="rluD"
                     /locus_tag="PKZ52_12005"
     CDS             764..1723
                     /gene="rluD"
                     /locus_tag="PKZ52_12005"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNG60539.1"
                     /translation="MRRRITLNHTVPYEASGQRFDQVSAQLFPQFSRARLQQWIKNGE
                     LTLNGQAFKGNGKLVGGEAMFLDVEVQDEGDWQAEAIALDVVFEDDTILVVNKPAGMV
                     VHPAAGNSEGTLLNALLHHSPQLAAIPRAGIVHRLDKDTTGLMVVAKTLEAQNHLVSQ
                     LQAREVSRQYQAIVVGRVISGGTINLPIGRDSKHRKKMAVSKTGEGKEAITHYEVAER
                     FSYFTRLCIKLETGRTHQIRVHMSHNRFPLVGDPAYGGGGKGLRSSVINEEVLEAVKA
                     FPRQALHAEALSLIHPQTEEQCQWQVPLPPDMLELLTLMRRYD"
     gene            1731..2498
                     /gene="pgeF"
                     /locus_tag="PKZ52_12010"
     CDS             1731..2498
                     /gene="pgeF"
                     /locus_tag="PKZ52_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNG60540.1"
                     /translation="MLAPDWPAPAQVKAVITQAQLAGEAAGFSQPPYGEFNLATHVGE
                     AIERVAANRQLLSARLGIGPVQWVEQVHGTEVFSARWPIVTPAPVADGVYTATPGLPI
                     AVLTADCLPVLLCTEAGDEVAAAHAGWRGLAQGVLAQTLNAFRAPAKVLIAYLGPAIG
                     PAHFEVGEEVLEVFRHSFAPYGDVGPCFVPGRPGHYLADLYRLARMQLSALGVTAIYG
                     GQDCTFADGRFYSFRRSGGPCGRMASLIWLDDSSSHP"
     gene            2522..3280
                     /locus_tag="PKZ52_12015"
     CDS             2522..3280
                     /locus_tag="PKZ52_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60541.1"
                     /translation="MPLMIAAIALLGRRFGGRAAGLMSGFPIIGGPIVYFVYWDQGAG
                     FALAAAKATLAGVVGLSSFSFVYAWLAPRFSWWVCLLAGWSVYSAIGIALAQAHLSAH
                     LYALIAVVTLLAQLRWGRPLAAVVSASQTSSAEIICRMLFAAALVLAVTLLARPLGAE
                     LAGVLAAFPIASSVIAVFSQRFYSPWHAVLALRALKQGLLSLLAFFYGLVVFGGTLSF
                     DAAFVLAALMAVLTQACVVLAASCNRCRAGRSAP"
     gene            complement(3287..3907)
                     /locus_tag="PKZ52_12020"
     CDS             complement(3287..3907)
                     /locus_tag="PKZ52_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60542.1"
                     /translation="MNKYFVSMAFIGLMGLGPLGWCSGLSAAKVPGFDSAQVLAKEAW
                     PHFNAIYLAPTQLAFNKQWLKDRGQKISSAEREQLQAQYSELMDKNVRERLRARGWQL
                     QPAPGPDTLTLSTNVTDFFLNAPDMEPTISKSYVRVAGFAQVEFTLSQTDGTVLAKLN
                     DYSETHEKPGGQLGLTNRGLNRWDFGHLMQRWSTRLTEFLPAPEQT"
     gene            4201..>4720
                     /locus_tag="PKZ52_12025"
     CDS             4201..>4720
                     /locus_tag="PKZ52_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNG60543.1"
                     /translation="MRLDRFTNQLQVALSDAQSLAVGRDHNQLDGLHLLHALIEQQGG
                     AVKPLLASAQFDLNRLRQLLIQGLDNLPRIKNPTGDVAMSQDLGRLLNLADKTAQQQG
                     DSFISSESVLLAAMNDGNNSMGKLLLSVGKLPALQKAIEKIRGGEKVDNPDAENNRQA
                     LTKYTVDLTARAE"
BASE COUNT          976 a         1227 c         1356 g         1161 t
ORIGIN      
        1 ccgccagtgc atcgggcact gctggagtgg tttgaaagtt ctctaccaca tagcggccgc
       61 ggttggcggc ggctaaatag gcgccccgtt taaaataata attcgccacg tgaatttcat
      121 agcgggccag cacattgcgc agatacacca tgcgtttttg ggcatccgga gcataggggg
      181 atttggggta gcgagttaaa aagtcggtaa attgcgctaa agaatcttta gctgcacctg
      241 ggtcgcggtt ggtgttgtcg atggataata aagacgagag aaaactgcgt tctttattaa
      301 aactcgccat accgcgcata tagtaggcgt agtcggtatt gcggtgttgc ggatgcaagc
      361 gaataaagcg gtcagctgcg gctatagcct cgtcaaattg atccgcctcg tagtgggcgt
      421 aaataatttc cagctggctc tgctcaccat agctgccgaa gggatagtgt tcttctatta
      481 attgcagggc tttaatggcg ctgtcccagt tgctggcgtt gagggcgagc tgagcttttt
      541 cgtagagttt ttgttcggag ctaatttttt cttctttttt agaggagcag ccgctgatca
      601 ggctgaaaac cgcgagaaat ataacggcgc tggaaagttt aggtactcgc atggggcatg
      661 gctctgtatc attggcgggg gtggcaattg ccacccgaag ttgcgttgct taaggcctta
      721 gttggcaggg ggcgcagtgt agccagtagc taatgagaac tcaatgagac gccgaataac
      781 cctaaaccac accgtgccct atgaggctag cgggcagcgc ttcgatcagg tcagtgccca
      841 attgttcccc cagttttccc gcgcccgctt acaacaatgg attaaaaatg gcgaactcac
      901 cctcaacggc caggccttta agggcaatgg caaattagtg gggggcgaag ctatgttttt
      961 ggatgtggaa gttcaagacg aaggagactg gcaggccgaa gccatagctc tggatgtagt
     1021 atttgaagat gacaccattt tagtggtgaa caaaccggca ggcatggtgg tgcatccggc
     1081 ggccggtaat agtgaaggca ctttgctcaa tgccttgttg catcacagcc cccagctggc
     1141 agccataccc agagcaggca tagtgcaccg cctggataaa gacaccaccg gtttgatggt
     1201 ggttgctaaa acccttgaag cgcagaatca tctggtcagc cagctgcagg cgcgggaagt
     1261 ctcccgccag tatcaggcca tcgtggtggg ccgggtaatc agcggcggca ccattaattt
     1321 gcccatcggc cgcgacagta aacaccgcaa aaaaatggcg gtcagcaaaa cgggggaggg
     1381 caaagaggcc attacccatt atgaagtggc ggagcgcttt agttatttca cccgcctgtg
     1441 cattaagttg gaaacgggcc gcacccatca gatccgtgtg catatgagcc acaaccgctt
     1501 tcctttagtg ggcgatcctg cctatggcgg cggcggtaaa ggcctgcgct ccagcgtcat
     1561 taatgaagag gtgttagagg cggtaaaggc ttttcctcgc caggcattgc acgccgaggc
     1621 cctctccctc attcacccgc agactgaaga acagtgccag tggcaggtgc cgctgccgcc
     1681 ggacatgttg gaactgttaa cgcttatgcg ccgttacgac taggccgctt atgctcgctc
     1741 ccgactggcc agccccggcc caggttaaag cggtgattac ccaggctcaa ttggcggggg
     1801 aagctgccgg ttttagccag cccccctatg gcgagtttaa tttggctacc catgtggggg
     1861 aggccattga acgggtggcg gctaaccgtc aactgttgag tgcgcgcttg ggtataggcc
     1921 ctgtgcaatg ggtggagcag gtgcacggca ctgaagtatt cagcgcccgc tggcccatag
     1981 tgactcctgc acctgtggct gatggggttt ataccgctac acctggtttg cccatcgccg
     2041 tcttaaccgc cgattgcctg ccggtgctgc tgtgtaccga ggctggcgat gaagtcgccg
     2101 ccgcccatgc tggctggcgc gggctggccc agggtgttct ggcgcaaacc ctcaacgctt
     2161 ttcgtgcgcc agccaaagtg cttatcgcct atttaggccc agccattggg cccgctcact
     2221 ttgaggtggg ggaggaagtg ctggaggtat ttaggcattc attcgccccc tatggtgatg
     2281 tcggcccttg ttttgttcca ggccgccctg gtcactacct ggctgatttg tataggcttg
     2341 ctcgtatgca gttatcggcg ttgggggtaa cagccattta tggcgggcag gattgcacct
     2401 ttgctgacgg gcggttttat tccttccgcc gcagtggcgg accctgtggc cgcatggcga
     2461 gcctgatttg gctggatgat tccagttcac acccctgatg ctgctggcaa agctgttttt
     2521 tgtgccgctg atgattgcgg ccatagccct gttggggcgg cgttttggcg gccgggcggc
     2581 agggcttatg tcgggctttc ccattatcgg cggccccatt gtgtattttg tttattggga
     2641 ccaaggggcg ggtttcgccc tggccgccgc caaggccacc cttgctgggg tggtgggttt
     2701 atcctcgttt agttttgttt acgcctggct tgcgccgcgt tttagctggt gggtttgcct
     2761 attggcgggc tggagcgtct actctgccat aggtattgcc ctggcccaag cccacttaag
     2821 tgcccacctg tatgcgctga tcgctgtggt taccttgttg gcgcaattgc gctggggaag
     2881 gccgctagca gcagtggtta gtgcaagcca aaccagcagc gccgaaatta tctgccgcat
     2941 gttatttgct gctgcgttgg tgttagctgt cactctacta gcgcggccct tgggggcgga
     3001 attggccggc gtgctggctg cctttcccat cgcctccagt gtgattgcgg tgttctccca
     3061 gcggttttac tcgccctggc acgcggtact ggcgctgcgg gctctgaaac aggggctttt
     3121 gagcttactg gcgttttttt acgggctggt ggtgttcggc ggcaccttga gttttgatgc
     3181 ggcatttgta ttggcagctc tgatggccgt gcttacccaa gcctgtgtgg tgttggccgc
     3241 tagctgcaac aggtgccggg cgggccgctc ggcaccctaa acagtcttag gtttgttctg
     3301 gtgccggtag aaactccgtc aatcgcgtag accagcgctg cattaaatgg ccaaaatccc
     3361 agcggttcaa accgcgatta gtaagcccta attgcccgcc gggcttttca tgggtttcgc
     3421 tatagtcgtt taatttggca agcactgtgc cgtccgtttg gctgagggta aattccacct
     3481 gagcaaaacc cgccacccgc acataacttt tgctgatggt gggctccata tccggcgcat
     3541 tgaggaaaaa atctgtgacg tttgtgctaa gggtcagggt atctggccct ggggccggtt
     3601 gcagttgcca gccccttgca cgcaaacgct cgcgcacatt tttatccatt agctcactgt
     3661 attgagcctg caattgttcc cgctcggcgc tggaaatttt ttgcccgcga tctttcaacc
     3721 actgcttatt aaaggccaat tgagtggggg ctaaataaat ggcattaaag tggggccagg
     3781 cttctttggc gagtacctgg gctgaatcaa agcctggcac cttggccgcg cttaaaccac
     3841 tgcaccaacc cagcggtccc aggcccataa gcccaataaa agccatgctg acgaaatatt
     3901 tattcataag tcacctcgat aaaacagcgg tcttagtggg aagccttagt ttaaggaaag
     3961 ttcgggattg agtgtgagac ccctctgccg aattagtgtt tgcataaaac ccacgctgcg
     4021 gtttccgctg cgggctcgcc gtccgcaacg ccttcgcaaa caccaattgg ttaggggcct
     4081 acaccaaaaa ttctgtgctt gcggtttttg cgctaaatca agcctcgccg cctgaattac
     4141 ttgaattcat cgccaccgcc cccagcttga gttcaaccca ccatgaggac ttaattcgtt
     4201 atgcgtcttg accgttttac taatcaacta caagtggcgc tctcagatgc ccagtcactg
     4261 gccgtcggcc gcgaccataa tcagctggac ggcctgcatt tattgcacgc gctgatcgaa
     4321 cagcagggcg gtgcggtaaa acccctactg gcaagtgcgc agtttgatct taaccgcctg
     4381 cgccaactgc tgattcaagg cttggataat ttaccgcgca ttaaaaatcc cactggcgat
     4441 gtggccatgt cgcaggattt aggccgctta ttaaaccttg ccgataaaac cgcccagcag
     4501 cagggggata gttttatttc cagcgaatcc gtgttgttag ccgccatgaa cgatggcaat
     4561 aacagcatgg gtaaattatt gctcagcgta gggaaattgc cggccctaca aaaagccatt
     4621 gaaaaaatac gtggtggtga aaaagtggat aaccctgatg ctgaaaataa ccgccaagcc
     4681 ctaacaaaat acactgtaga tttaactgcg cgggccgagg
//
LOCUS       DAOQGS010000320         4712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9558_length_4712_cov_5.214516, whole genome shotgun sequence.
ACCESSION   DAOQGS010000320 DAOQGS010000000
VERSION     DAOQGS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_4712_cov_5.214516"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKZ52_12030"
     CDS             <1..557
                     /locus_tag="PKZ52_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG60544.1"
                     /translation="NFALGGQLIRCNALPDHDFAAIYGADQLVAVGSWRDLTICGQTA
                     CLKPGVIISLDGIAKGFAVDAAVKLIQAAHVQSGWINAGGDIRVFGEQVLAVSIRDHE
                     GNLHAKGGLRDAAIATSTTCASAEFPGWVLDAQGQSLAAATIAVIAHKAWRADALTKV
                     AAATPVAQRADVVGRLGGRWVPVP"
     gene            663..1091
                     /locus_tag="PKZ52_12035"
     CDS             663..1091
                     /locus_tag="PKZ52_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60545.1"
                     /translation="MKKSRINLILSFAVLAFSGLALVLLAPSEDGLPHPLNGVLREIH
                     GVGAAFGLFMFGYMFSDHVKKKLVRYRKTRAPRPWDGYLHLWLWVALIVSGLLLYYPQ
                     EIAGESPISMAKAHWLAGVGLIFLLPLHVGRNYIFNRAGK"
     gene            complement(1485..2069)
                     /locus_tag="PKZ52_12040"
     CDS             complement(1485..2069)
                     /locus_tag="PKZ52_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNG60546.1"
                     /translation="MKLLPLFTLGCLLTGNALAYEAGDIIARAGVATVDPAGDGALNG
                     ALDVKSNSQLSLTLTYMLSEDWGVGVLAATPFKHDITLAGKTVGSTKHLPPTLTAQYH
                     FSLGESIHPYVGLGVNYTQFFSEESALGDLSLDSSFGLAAELGADFTFNDHWGANIAL
                     WYIDIDTDASLDGNKLATVQIDPWVYNLGLSYRF"
     gene            complement(2480..2974)
                     /locus_tag="PKZ52_12045"
     CDS             complement(2480..2974)
                     /locus_tag="PKZ52_12045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG60547.1"
                     /translation="MSLVLSAPEPLTAHHRVDNFSCGEPALDEWLKKRAHGNQLTGAS
                     RTFVVADQDNQVQGFYALAAGAVVHADATGAVRRNMPDPIPVMVLARLAVNQKAQGIH
                     LGAALLQDAVKRAQMVAQNAGVRALLVHAINDTAKQFYTRYGFQESPLQPLTLLLKLP
                     IVAP"
     gene            complement(2971..3240)
                     /locus_tag="PKZ52_12050"
     CDS             complement(2971..3240)
                     /locus_tag="PKZ52_12050"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNG60548.1"
                     /translation="MRAASINLRALPEQRALIDLAAHALGKNRSDFMLEAACERAQAV
                     LLDQVFFALDDERYQQFTALLDTPTSSNPALVQLLAVKAPWNDKA"
     gene            complement(3419..4270)
                     /locus_tag="PKZ52_12055"
     CDS             complement(3419..4270)
                     /locus_tag="PKZ52_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60549.1"
                     /translation="MYIALTGTEDDLHKLETLTLQNAVCIDLKNSFTSNLKLSLEAVS
                     TPFYVGLLASGLSQSKLIENFAKQGAAGCLQSYETAKTLLDNKSYLTGIQSIIPTFQA
                     MHEYGLANNQFQALYKLVAMRFYGALEDLSKQALKIIITKNICNLDILSAQGLKIAKS
                     DCVKLKRRIIQQQQEHSLTIEHAYDEEISHLSYQVFQYTELFSALMNFSIPDNIIDLL
                     TRLESSRHLFTHRNSIVDQKYINEVGNNLEIGDTLHASPDDMYRWQNAIIDYSAALLE
                     SLDTAYS"
     gene            complement(4417..>4712)
                     /locus_tag="PKZ52_12060"
     CDS             complement(4417..>4712)
                     /locus_tag="PKZ52_12060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG60550.1"
                     /translation="NAAPATNRAVSGALGSLVSQAQAAYQAGNYQTAISTAERGLRID
                     RRAPALYLVMAQSYLALNQPTQAAQFANQGLRFSQSGSAEARALEKVRNGAGE"
BASE COUNT         1101 a         1049 c         1248 g         1314 t
ORIGIN      
        1 ttaattttgc cttgggcggg caattaattc gctgcaacgc gctgcctgac cacgactttg
       61 ccgcgattta tggcgccgat cagctggtgg cggttggctc ctggcgggat ttaaccatct
      121 gtgggcaaac cgcctgttta aagccggggg tgattattag tttggacggc atcgctaagg
      181 ggtttgcggt tgatgcagcg gttaagctta ttcaagccgc ccatgttcaa tccggctgga
      241 tcaatgcggg gggcgatatt cgcgtgtttg gcgagcaggt tttagcggtg agtattcgcg
      301 accatgaggg caatctgcac gctaaaggcg gtttgcgtga tgccgccatt gccacctcaa
      361 ccacctgcgc cagtgcggaa tttcccggct gggttttgga tgcacagggc cagtcattag
      421 cggctgcaac catcgcagtg attgcccata aggcctggcg ggccgatgct ttgaccaagg
      481 tggccgccgc aaccccggtg gcccagcgcg ctgacgttgt gggccgcttg ggcgggcgct
      541 gggtgcccgt gccctaatcg ggtagtattt ctctgcattt cgacggatgt gtgcaaaacc
      601 gcgcccccag ctgcaggcca aatcgggggc tgtttattat tttgaatacc aaaacgatgt
      661 atatgaagaa atcccgtatt aatttaatcc ttagttttgc ggtgctggcg ttttcagggc
      721 tggcacttgt gttgctggct ccgagtgaag atggcctgcc ccatccttta aatggtgtgc
      781 ttagggagat acacggcgta ggcgccgcgt ttggcctatt tatgtttggc tacatgtttt
      841 ctgatcatgt gaaaaaaaag ctcgttcgct accgcaaaac acgcgcccct cgcccttggg
      901 atggttatct acatctgtgg ttatgggtag ctttaattgt gagcgggctg ctgctgtact
      961 acccgcaaga gatcgcaggg gaaagcccta tcagcatggc taaggcccat tggcttgccg
     1021 gtgtgggttt aatatttttg ttgcccttgc atgtgggccg taactatatt tttaatcgcg
     1081 cgggaaaata acggtgctaa agatagaatt agtacctgta ctggatatgt tgctggcgcc
     1141 gactggaaag tgttttagcg ctgccatgcc tcaacactcc gcccgcagtg ttgaattcag
     1201 gttagtgcag atacgccgtg aatacatccg tgtaggctcg ccataaagat ccctcttttt
     1261 gacggtctgc actaacctgt attcaccact gctacaccgc caattgattc tgccgcttgg
     1321 ttaaatttct cttaatttgc cgttcactta ggttaaggta ataaggcgtg ttgagctgaa
     1381 aagctgaaaa gctgaaaagc tgaaaagctg aaaagctgaa aagctgaaaa gctgaaaatg
     1441 ttacgctaca aaaaggcctg cgcactcagc aggccataaa ttaattaaaa acgataactt
     1501 aagcctaggt tatacaccca agggtcaatc tggaccgtgg cgagtttgtt tccatccagg
     1561 cttgcatccg tatctatatc gatataccac agcgcaatat tcgcgcccca gtggtcgtta
     1621 aacgtaaaat ctgcacctaa ctccgccgct aaaccgaaag aagaatccag tgataaatca
     1681 cccagtgccg attcttcgct aaaaaattgc gtgtaattaa ctccgaggcc gacataggga
     1741 tgtatgctct cgcccaacga aaaatgatat tgcgcagtaa gggtgggcgg taaatgtttg
     1801 gtgctgccga cggttttgcc ggccagagta atatcgtgtt taaacggagt ggcggctaat
     1861 acacccacac cccaatcctc gctgagcata taggtgagcg tgaggcttaa ttggctgttg
     1921 gattttacat ccagcgcgcc gtttaacgcg ccgtcgcctg ccgggtcaac ggttgccaca
     1981 cccgcacgcg caataatatc gcccgcttca taggccagtg cattgccggt taataaacag
     2041 ccgagggtaa ataagggtaa taatttcata tggatactcc taaggttttg gggcaatttt
     2101 aggcgtaaca aatccaggtt atttgattga tgtcaggtaa ttgctgcggg tgcggtgttt
     2161 tgacgatgct tagggacgaa tttttgattt gggttaaaaa ataaattctt tttatctttt
     2221 tgcggcagaa ttgtgcgctg tgagttaatc aacacataat cctaaaaact tcagttgaat
     2281 ctttttgcga atacctaaca cttgaaaacg cctcaattcg gcgtcttagc cgctccagct
     2341 gcgagcgcaa ctgaagtttt tagataattg atgttgcata aaacgattgt attttgattg
     2401 gaaatagaga attaactgcg cgatgtgcgc gtatttggca gtgtgggatc gcgaaatatg
     2461 tcggcgcggc tgatattttt taaggtgcaa caataggtaa ttttagcaat aaggtgagcg
     2521 gctgcagagg agattcttga aagccgtagc gggtgtaaaa ttgtttggcg gtatcgttaa
     2581 tggcgtggac cagtaaagcg cgcaccccag cattttgcgc gaccatttgc gcgcgcttta
     2641 ctgcatcctg caacagcgct gctcccaaat gaataccctg tgccttttga tttaccgcaa
     2701 gccgagccaa caccatgacc gggatggggt cgggcatatt tcggcgcacc gcgccggttg
     2761 catccgcgtg aaccacggcc ccagcggcta aggcgtagaa tccctgcacc tgattgtctt
     2821 gatcagctac cacaaaggtg cggcttgcgc cggttagttg gttgccgtga gcgcgctttt
     2881 taagccattc atccagtgca ggttcaccac agctgaaatt atcaacgcgg tgatgtgcag
     2941 ttaagggctc aggcgcgcta agtaccaaac tcacgccttg tcattccagg gtgccttcac
     3001 tgcgagcaac tgtacgaggg ctgggttgct gctagtaggg gtgtcaagca gggcggtaaa
     3061 ttgctggtaa cgctcgtcat caagggcaaa aaatacctga tccaacaaca cagcttgggc
     3121 gcgctcgcag gctgcttcaa gcataaaatc ggagcggttc ttacccagcg catgggcggc
     3181 taagtcaatc agtgcccgtt gctcaggcag tgcccttaaa ttgatggagg cggcgcgcat
     3241 tgttatggac tcttcgtgtg ttgacaatag atatacagga tggtcttgtg tgtagctgtt
     3301 gtcaatacgg aatttgtgtt tggggtgttt gattttaggt ttaggctgcg cgaacaagaa
     3361 tgaatttatg cggtggactt ttctgcggga gatgtactta catgcaggag cgaggaaact
     3421 aagaatatgc ggtatctaaa gattcaagta gtgcggcaga ataatctatt atcgcgtttt
     3481 gccagcggta catatcgtcc gggctagcat gcaacgtgtc tccaatttct aaattgtttc
     3541 cgacctcatt gatatacttc tgatccacaa tagaatttct gtgagtaaat aaatgtctgc
     3601 tcgattctag tcgtgttaat aaatcaataa tattgtctgg gattgagaag ttcataagtg
     3661 cagaaaataa ttctgtgtat tgaaagactt gataactcag gtgtgagatc tcttcgtcat
     3721 atgcgtgttc tattgtgaga gagtgctctt gctgttgttg aataattctt cttttgagtt
     3781 tgacgcaatc cgattttgct attttcagtc cttgtgctga tagtatgtct aaattgcata
     3841 tgttcttagt gatgatgatt ttaagcgctt gtttgcttag atcctcaagt gctccataga
     3901 atctcatggc gactaattta tatagcgctt gaaattggtt gtttgctagt ccatattcgt
     3961 gcatcgcttg gaatgtggga attatggact gaatgccagt taaatagctt ttgttatcta
     4021 gtagtgtttt agctgtttcg tagctctgca aacagccggc agctccctgc ttggcaaagt
     4081 tctcaataag ttttgactga ctcaaaccgc tcgccaataa gccaacataa aaaggtgtgc
     4141 tcacggcttc aagagaaagt tttaaatttg atgtgaaact gttttttaag tctatgcaaa
     4201 ctgcattctg tagggtaagt gtttctaatt tatgtaagtc gtcttcggtg ccagttaagg
     4261 caatatacac ataaaattct cgtcttaaat cttgataagt ccatgccgat tgcataaatt
     4321 tttggcgcca gttcggtgta aattgctttg atgattgatc gaccagatgt agtcaagatt
     4381 gtcatcagtc ggtattggca tcacttaatg cctcaattac tccccagccc catttcgtac
     4441 tttctccagc gcccgcgcct cggcggagcc gctttggcta aagcgcaggc cctggttggc
     4501 aaactgcgcc gcttgggtgg gttggttgag ggcgagataa ctctgcgcca tgaccaggta
     4561 gagggcgggt gcccggcggt cgatgcgcag gccgcgttcg gcggtgctga tggcggtttg
     4621 gtaattgccc gcctggtagg ccgcttgagc ttggctgact aagctgccca gggcgccgct
     4681 caccgcccgg ttggtggcgg gggcggcgtt gg
//
LOCUS       DAOQGS010000321         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9565_length_4710_cov_4.028786, whole genome shotgun sequence.
ACCESSION   DAOQGS010000321 DAOQGS010000000
VERSION     DAOQGS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9565_length_4710_cov_4.028786"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="PKZ52_12065"
     CDS             <1..1299
                     /locus_tag="PKZ52_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNG60551.1"
                     /translation="VGRIEANILERVTEVERSARQYNLLRDPKFQTFLQNDLTLLAED
                     VAKLQQTLPQAAGPIGHFLAASQQLLSAAEAEPATLASRLQELRDQRDQLVEFWDAYS
                     AEQLNAAQARFNSTLRWLLILGAWALPGTLLLVALSVAAVGAPLRRLASAIHDLGHAR
                     WQRPIAIEGPKDLRELGENLEWMRQRLALSEQHKQAFLQDITHELKAPLAAITEASRL
                     LNEQVLGSTTPGQEEVIRILLENSQSLHELIQQLLNYNAVTQGLLADVHAFDLTAMSQ
                     KLCAKITAARPHSQCSWHLPEEPFYVNSDPQLISMILNNLLSNAHDFSGQHGKVTLSW
                     GESPQGWWLSVADNGPGINKDELNKIFKPFYQGRARRHGSLKGSGMGLAIVAACTKLL
                     AGKIKVSSTLKKGTRFIVHFPINPEIVHEPPSFQTGSAPV"
     gene            1259..1882
                     /locus_tag="PKZ52_12070"
     CDS             1259..1882
                     /locus_tag="PKZ52_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60552.1"
                     /translation="MNHPVSRPALRLFKFHQWGGVVLLANCLQGCSTLPLPGETSSPT
                     NQAKLQPQRTLNELLAHRLTLCSLDKTQRAEQIKTLRGQLQHLRKDDSSNLEEELNGL
                     LLTSCEPASTPGLMGEVLNHLVNLGQWPVEYDNLFDLLRSQQRAISQYNARALDSSRE
                     NQELRQALQNQKEEYNKLQSSYKEAIKGIGDIEETLDSRKQKRLPPP"
     gene            1892..3226
                     /locus_tag="PKZ52_12075"
     CDS             1892..3226
                     /locus_tag="PKZ52_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNG60553.1"
                     /translation="MPQARVLLVDDDPSMLTLYSYRLQACNCVITTAQSGTEALEALN
                     KELPDLVITDLRMENMDGLALFDRIQAQWPSLPVIIFTAQGSIREAVNATQKGVFSFL
                     TKPPDKDELVTTINQALQLSRRINIPHGEWQALFSTRSAKMHALIDQAKLLASSDVNV
                     LISGESGAGKEVLARSLHRYGDRSDKPFVAINCGAMPGELLESELFGHVKGAFTGAAR
                     DHEGLFVAADGGVLFLDEIGDMPLVLQSKLLRVLQEKTVRPLGGTKDRAINVRIISAT
                     HQNLTEAIKNKTFREDLFYRLNVVNLKVPPLRERKEDIPLMVTTFLDALAKNHNMTKQ
                     LAPQALVLLLSYDWPGNVRQLKNVIEQVYALSTSAMISPLLIENAIPDLNRGDVKPLT
                     EAKQEFERDYVIGLLKATQGNMAQAAILAGRNRSDFYKICKRYDLDSDSFKS"
     gene            3347..3895
                     /locus_tag="PKZ52_12080"
     CDS             3347..3895
                     /locus_tag="PKZ52_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNG60554.1"
                     /translation="MHQFKTRLSRLIGATAIICSPCFAEFYAGGSVGLSQWDDAPKSE
                     GTAYEIYGGYQFSEFFAAEIALTDPGSFDYSPSYNETFGTDISGANYSLVLSLPLSDA
                     YGLYAKIGLLDYEVERSYNDRTQAKIRNQDLNYALGFQLNAVDNVWVTFDYRYYPLTF
                     KYAGQEMELNADTFSVGARFTF"
     gene            complement(4098..4379)
                     /locus_tag="PKZ52_12085"
     CDS             complement(4098..4379)
                     /locus_tag="PKZ52_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNG60555.1"
                     /translation="MSTDNAQWSVYMVRCGDGSLYTGIAKDVAKRFAAHQAQGPKCAK
                     YLKGREPLRLVYQERVGDKSAALKREYAIKQLPKHTKEQLLTQRHLVPG"
     gene            complement(4376..>4710)
                     /locus_tag="PKZ52_12090"
     CDS             complement(4376..>4710)
                     /locus_tag="PKZ52_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638204.1"
                     /note="catalyzes the phosphorylation of protein substrates
                     at serine and threonine residues in vitro; specific
                     substrate in vivo has not been identified yet; plays a
                     role in long-term cell survival and expression of surface
                     appendages; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stress response kinase A"
                     /protein_id="HNG60556.1"
                     /translation="DDALNGPAVQDLWMLLSGSPAAQQTQLVQVLEGYEEYRPFDTQE
                     LALIPSLRALRLMNYSAWLARRWEDRAFVAAFPWFNTHAYWAGHIKQLQEALHEINTL
                     RFHRGQPF"
BASE COUNT         1133 a         1368 c         1191 g         1018 t
ORIGIN      
        1 gtggggcgca ttgaggcgaa catccttgaa cgggtgacgg aagttgagcg ctcggcacgc
       61 caatacaacc tgctgcgcga ccccaaattt caaacctttc tgcaaaacga tttgacctta
      121 ctggcggagg atgttgccaa attgcaacaa accctgcccc aagccgcagg ccccatcgga
      181 cacttcctgg cggccagcca acagctgctc agcgccgccg aggctgagcc agctaccctc
      241 gccagccgct tacaggagct gcgcgaccag cgcgaccagc tggtggaatt ttgggatgca
      301 tacagtgctg agcagctcaa cgccgcccag gcgcgcttta acagcaccct gcgctggctg
      361 ttgattttag gcgcctgggc gctgccgggc accctgctgt tggtggccct cagcgtcgcc
      421 gctgtggggg cgcccctgcg ccgcctggcc agtgccattc acgacctggg gcacgcccgc
      481 tggcagcgcc ccatcgccat tgaagggccc aaagatttgc gggagctggg ggagaacctt
      541 gagtggatgc gccagcgcct agcgctcagc gaacaacata aacaagcgtt tttgcaggat
      601 attacccacg aacttaaagc ccctttggcc gccatcactg aggcctcacg gctgctcaat
      661 gaacaggtgc tcggcagcac cacaccaggc caggaggaag tcattcgcat actgctggaa
      721 aactcccaaa gcctccatga attaattcag cagctgttga actacaacgc cgtgacccag
      781 gggctgctgg ccgatgtgca cgcctttgac ttaaccgcca tgagccaaaa actctgcgcc
      841 aaaatcaccg ccgcccgccc ccacagccaa tgcagctggc acctgccgga agagcccttt
      901 tacgttaatt ccgacccaca attaatctcc atgattctca acaacctatt atccaatgca
      961 cacgactttt ccggccagca tggtaaagtc actctcagct ggggcgagag cccacagggc
     1021 tggtggctga gtgtggcgga taatgggccg gggattaata aggacgaatt aaataagatt
     1081 tttaaaccct tttaccaagg tcgcgcccgc cgccacggct cgcttaaggg cagcggcatg
     1141 gggcttgcca tagtcgccgc ctgcaccaaa ctgttagcag gcaaaataaa ggtcagctcc
     1201 acccttaaaa aaggcacgcg ttttattgtg cacttcccga tcaaccccga gattgttcat
     1261 gaaccaccca gtttccagac cggctctgcg cctgtttaag tttcatcaat ggggcggagt
     1321 tgtgctgttg gccaactgcc tacaaggctg cagcaccctg cccctccccg gcgagaccag
     1381 cagccccacc aatcaggcca aactgcaacc tcagcgcacc ttgaacgagc tgctggccca
     1441 ccggcttacg ctgtgctcac tggataaaac ccagcgcgcc gaacagataa aaaccctgcg
     1501 cggccagctg caacacctgc gcaaggacga cagcagcaac ctagaagagg aattaaacgg
     1561 cctgctgcta accagctgtg agccagccag cacaccgggg ctgatgggcg aagtgctcaa
     1621 ccatttggtc aacctaggcc agtggcccgt cgaatacgat aacctgttcg acttattgcg
     1681 cagtcaacag cgcgccataa gccaatataa tgcccgcgcc ctggacagca gccgggaaaa
     1741 ccaagagctg cgccaagcgt tgcagaacca gaaggaggag tacaacaaac tgcaaagctc
     1801 ctacaaggag gccatcaaag ggatcggcga tattgaagag accctagatt cccgcaaaca
     1861 aaaacgactg ccaccccctt aaggaaccgc catgccccaa gcgcgtgttt tattggtcga
     1921 tgacgacccg agtatgctga cgctatattc ctaccgcctg caggcgtgta attgcgtcat
     1981 caccaccgcg caatcgggca ccgaggcatt ggaggccctc aacaaagaac tgccagacct
     2041 ggtaatcacc gatttgcgca tggaaaacat ggacggtttg gccctgtttg atcgcatcca
     2101 agcccagtgg ccgagcctgc cggtgattat ttttaccgcc cagggctcca ttcgcgaggc
     2161 ggtcaacgcc acgcaaaaag gcgttttctc gtttttaacc aaacccccag ataaagacga
     2221 gctggtgacc accatcaacc aggccttgca attgagccgc cgtattaata ttccccacgg
     2281 cgagtggcag gcgttattca gcacccgcag cgccaaaatg cacgccctca tcgaccaagc
     2341 taaattgctc gccagctccg atgtaaacgt gttaatcagc ggcgagagcg gcgccggcaa
     2401 agaagtactc gcccgcagcc tgcaccgcta cggcgatcgc agcgacaaac cctttgtcgc
     2461 catcaactgc ggcgccatgc cgggggaatt gttggagtcg gaattattcg gccatgttaa
     2521 aggcgctttt accggcgctg cgcgggacca tgaaggctta tttgtagccg ccgatggcgg
     2581 cgtattgttt ttagatgaaa tcggcgatat gccgctggta ttacaaagta agttgctgcg
     2641 ggtgttacag gaaaaaactg tgcgcccgct cggtggcact aaggatcgag cgattaacgt
     2701 gcggattatt tccgccaccc accaaaattt gacggaagcg attaaaaaca aaacctttcg
     2761 ggaagattta ttttatcgct taaatgtagt gaatttaaaa gtgccacccc tgcgcgagcg
     2821 caaagaagat attcccttaa tggtcacaac gtttttggac gcgctggcta aaaaccacaa
     2881 catgaccaaa cagttggcgc cgcaggcgct cgttttattg ttgtcctacg actggccagg
     2941 caatgtgcgg cagttaaaaa atgtcattga acaggtgtat gcactctcca ccagcgcgat
     3001 gatttcaccg ttattaattg aaaatgccat ccccgattta aaccgcggcg atgtcaaacc
     3061 cctcaccgaa gccaaacaag aatttgagcg ggattatgtg attggcctgt taaaagccac
     3121 ccagggcaat atggcccaag ccgctatttt ggccggtcgc aaccgctcgg atttttacaa
     3181 aatttgcaaa cgctatgatt tagacagcga cagttttaaa agctaggcct gcgcaccaat
     3241 tattaacccg gcactaaacg ccgccgcgtt aacggctatt tttcagcccg cattcagctt
     3301 ttaaagcgca gattgagcgc gcccttggca accttttgga gacactatgc accagtttaa
     3361 aacccgttta agccgcctga taggcgccac cgcaattatc tgcagcccct gttttgccga
     3421 attttatgcg ggcggctcgg tgggcttaag ccagtgggat gacgccccta aatccgaagg
     3481 caccgcctat gaaatttacg gcggctatca attttctgaa ttcttcgcag ctgaaattgc
     3541 cttaaccgat ccgggcagtt ttgactacag ccccagttac aacgaaacct ttggcaccga
     3601 catctcaggt gctaattatt ctttggtgtt atccctgccc ttaagcgatg cctacggttt
     3661 atatgccaaa atcggcctgc tcgattatga agtggagcgc agctataacg accgcaccca
     3721 ggccaaaatt cgcaaccagg atcttaatta cgccctgggg tttcaattaa atgcggtgga
     3781 taatgtatgg gttacttttg actaccgcta ttacccgctc acctttaaat atgcgggtca
     3841 agagatggag ttaaatgcgg acacctttag tgtgggggcg cgttttacct tttaatgcgg
     3901 catttatttc tacggttgca tccgcacatg caattaacta aaggcagctt aggctgcctt
     3961 ttttattggc gaaactcagc cagcggcttt taacccggcg aaaaataaga tagtcctgat
     4021 ctatttttct tgtgctgcgc taaaactcgc ggattttctt gttttagctc agctcgcagc
     4081 ggcttgcgcc gccattatta acccggcact aaatgccgct gggttaataa ttgctcttta
     4141 gtatgtttgg gcaattgttt tatcgcatac tcgcgtttaa gcgccgctga tttatccccc
     4201 acccgctcct gatacaccaa acgcagcggc tcgcgccctt ttagatattt ggcgcactta
     4261 ggcccttgag cctggtgcgc ggcaaagcgt ttggccacgt ctttggcaat accggtgtac
     4321 aaacttccat cgccacagcg caccatgtac acgctccact gggcattatc ggtgctcaaa
     4381 acggctggcc ccggtgaaaa cgcagggtgt ttatctcgtg cagcgcctcc tgtagctgct
     4441 tgatatgacc ggcccaataa gcgtgggtgt taaaccaggg aaaagccgcc acaaaggcgc
     4501 gatcctccca gcggcgggcc agccaagcac tgtaattcat caggcgcagc gccctaaggc
     4561 tggggataag ggccagctcc tgggtgtcga aggggcgata ttcttcatag ccttccagca
     4621 cctgcaccag ctgcgtctgc tgggcggcgg ggctgccgct taataacatc cacagatcct
     4681 gcaccgctgg gccgttgagc gcatcgtcac
//
LOCUS       DAOQGS010000322         4695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9605_length_4695_cov_5.339440, whole genome shotgun sequence.
ACCESSION   DAOQGS010000322 DAOQGS010000000
VERSION     DAOQGS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9605_length_4695_cov_5.339440"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKZ52_12095"
     CDS             complement(<1..468)
                     /locus_tag="PKZ52_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60557.1"
                     /translation="MSTAQPAAQLKAVSSLALLYMFRMMGLFMVFPVMTLYGMHYIDS
                     TEQRLGFAIGAYGLSQAVCQMPLGLLSDLYGRKTIIFIGLLIMIAGSVVAALSDSVWG
                     LIIGRALQGIGAVGSTIMALVGDLTTEQNRTKAMAVIGASIGVSFVIAMVLGST"
     gene            689..1342
                     /locus_tag="PKZ52_12100"
     CDS             689..1342
                     /locus_tag="PKZ52_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNG60558.1"
                     /translation="MTASPAPTSPTVQGIILFSISSSLQTGLLAQLIGDKTGLKCHVS
                     TQFSPDLEGAYLYLVDCQGRSIDDLQALVAEIQEHNQPYIAALLNATTESEHEQLLDW
                     PCISGLFSTDTDQENLLRGLEALLKGEYWVPRRLLHLHFEKNRRAPSSKRLDIKLTKR
                     ERQILKLIKDGATNFDIAEALEVSEHTVKSHLYNVYKKIGVRNRLEASNWVRDLEDI"
     gene            1580..4414
                     /gene="uvrA"
                     /locus_tag="PKZ52_12105"
     CDS             1580..4414
                     /gene="uvrA"
                     /locus_tag="PKZ52_12105"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNG60559.1"
                     /translation="MVDTITVRGARTHNLKNIDLDIPRDKLIVITGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLSLMEKPDVDHVEGLSPAISIEQKSTSHNPRSTVGTI
                     TEIYDYLRLFFARIGEPRCPTHQLPLSAQTISEMVDQVLAMPEDSKVMLLAPVVRDRK
                     GEHHHVFESLRRDGFVRVRVDGLVVDLDDTPALDKKKKHSIDVVVDRFKVRPDIKQRL
                     AESFETALNLADGIAMVASMEGEEEEKLFSAKHACPLCDYSLSELEPRIFSFNNPAGA
                     CPECDGLGVKQFFAPEKVVVDAQLSLAEGAVRGWDARNFYYFHILTSLAKHYKFDVDV
                     PWQKLSAKHQKVILYGSGEETVNFTYVNERGDKFQRSHAFEGVIPNLERRYRDTDSSG
                     VREELAKYLSSQVCPSCNGARLRLEARHVFVDNKTLPELVDMPVGDAHGYFTKLKFTG
                     AKGKIAEKILKELRDRFRFLVDVGLNYLTLSRSADTLSGGEAQRIRLASQIGAGLVGV
                     MYILDEPSIGLHQRDNDRLLNTLKHLRDLGNTVIVVEHDEDAIRLADHIIDVGPGAGV
                     HGGQIIAQGKLAQIVKAKNSLTGAYLSGKKSIAIPKTRHAPDKKKMLVLSGAAGNNLK
                     NVTLEIPVGLFTCVTGVSGSGKSTLINNTLHPLAATALNNATTLEAAPYKSIEGLDLF
                     DKCVDIDQSPIGRTPRSNPATYTGIFTPIRDLFAATAEARSRGYKAGRFSFNVKGGRC
                     EACQGDGVVKVEMHFLPDVYVPCDTCKGKRYNRETLDVHYKGKNIHEVLDMTVEDARA
                     FFDAVPSIASKLQTLMDVGLSYIRLGQAATTLSGGEAQRVKLSRELSKRDTGKTLYIL
                     DEPTTGLHFHDIAQLLAVLHRLRDNGNTVIVIEHNLDVIKTADWIVDLGPEGGGGGGQ
                     IIATGTPEAICKVKASHTGRFLKAVLEAKK"
BASE COUNT         1197 a         1243 c         1162 g         1093 t
ORIGIN      
        1 ggtggagccg agcaccatgg cgataacaaa ggacacgccg atggacgcgc cgatcaccgc
       61 catggctttg gtgcggttct gctcggtggt caaatccccc accagggcca taatggtgct
      121 acccacagcg cctataccct gcaaagcccg gccgataatc aacccccaga ctgaatcaga
      181 aagcgccgcc accacactgc ccgcaatcat aatcagcagg ccgataaaaa taatggtttt
      241 gcgcccgtac aggtcggata gaagccccaa gggcatttgg cagaccgctt ggctgaggcc
      301 ataggcgcca atcgcaaacc ccaagcgttg ctctgtactg tcgatatagt gcattccata
      361 tagggtcata acagggaaga ccataaacaa ccccatcatg cgaaacatat agagaagagc
      421 aagagaagaa actgctttta actgcgcggc cggttgagct gtggacacaa tagctactcc
      481 gaaaaaaatg ccgcgattct atcagcacac aggccccctc ggtggaacga tttcgcgatt
      541 cgccgcagcg caagcgagcg tcttatctgc tgaaaattag cgttgagcta gtgacactga
      601 aggtgtttca gttacaatct gtgcgatcat ctatgcaact tcagcgcaca gaattccagg
      661 cccccattag ccaaggcaga caaccatcgt gaccgcatcc ccagccccta cttccccgac
      721 tgttcagggc attattcttt tctcgattag cagcagtttg caaactggat tattggcgca
      781 attgatcggc gataaaacgg gccttaagtg ccacgtctca actcaattct caccagattt
      841 agaaggcgct tacctgtatt tagttgattg ccagggccgc tccattgacg atttacaagc
      901 gctagtagcc gaaattcaag aacacaatca gccttacatc gccgcattgc tcaatgccac
      961 tactgagagc gagcacgagc agttactcga ctggccctgt atttccggcc tgttctccac
     1021 cgataccgac caagaaaatt tgctgcgcgg ccttgaggcg ctgcttaaag gcgaatattg
     1081 ggtgccgcgc cgcctgctgc atttgcattt tgaaaaaaat cgccgcgcac cctccagcaa
     1141 acgcctggac attaaactca ccaaacgcga gcggcagatt ttaaaattga ttaaagatgg
     1201 cgccactaac tttgatatag ccgaagccct agaagtgagc gaacacacgg ttaaaagcca
     1261 cctgtacaac gtgtacaaaa aaatcggcgt gcgcaatcgt ttagaggcaa gcaactgggt
     1321 gcgggattta gaagacatct aagcctatcc tccctgccgc cccctacagg cggcaatccc
     1381 ttgcttgatt tcccctcctg tcactcaact cttttttctg ccgcctcacc caaccattac
     1441 acttccaacc gaaattctgc tgtcacttca tccaacccct gtgtaaaagc gtaatcacgc
     1501 gcctcgcctt ttgcgcctgt atgccgggca gaaaaagcgt attatcgccg tttggcttaa
     1561 attaatgttc cgaggattta tggtagatac catcaccgtg cgcggtgccc gcacgcacaa
     1621 tttaaaaaat attgaccttg atattccccg cgacaaatta attgtaatca ccggtttgtc
     1681 cggttcgggc aaatcctctt tggcctttga caccctctac gccgagggcc agcgccgcta
     1741 tgtggagagt ctgtccgcct acgcgcggca atttctctcc ctgatggaaa aacccgatgt
     1801 ggatcatgtg gagggtttaa gcccggcgat ttccatcgaa caaaaatcta cctcccacaa
     1861 cccgcgctcc accgtgggca ccattacgga aatttacgat tatttgcggc tttttttcgc
     1921 ccgtatcggc gagccccgct gccccaccca ccagctgccc ttaagtgccc aaaccatttc
     1981 ggaaatggtg gaccaagtgc tggcgatgcc cgaggacagc aaggtgatgt tgctggcgcc
     2041 ggtggtgcgc gatcgcaaag gagagcacca ccacgtcttt gaaagcctgc gtcgggacgg
     2101 ttttgtgcgg gtgcgggtgg atggtttagt ggtggattta gacgatacgc ccgcgttgga
     2161 taaaaagaaa aaacacagca ttgatgtagt agtggatcgc tttaaagtgc ggccggatat
     2221 taaacagcgc ctggcagaga gttttgaaac ggcgctgaat ttagccgatg gtattgccat
     2281 ggttgccagc atggagggcg aggaggaaga aaaattattt tccgccaaac acgcctgccc
     2341 gctgtgtgat tattcactgt cggaattgga gccgagaata ttttcgttta acaacccagc
     2401 cggggcctgc ccagagtgtg atggcctagg agtaaaacaa ttttttgccc cagaaaaagt
     2461 tgtagtggat gcacaactga gccttgccga aggcgccgtg cgcggttggg atgcgcgcaa
     2521 tttttattat tttcatatac tcacctccct ggccaaacac tataaattcg atgtggatgt
     2581 gccctggcaa aaactcagcg ccaaacatca aaaagtgatt ttatacggct caggcgagga
     2641 aaccgttaat ttcacttatg tgaatgaacg gggcgataaa ttccaacgct cccacgcctt
     2701 tgaaggcgtc attcccaatt tagagcgccg ctaccgcgac acggattcct ctggcgtgcg
     2761 cgaggaatta gccaaatacc tatccagcca ggtctgcccc agttgtaatg gtgcgcgcct
     2821 gcgcttagag gcgcggcatg tatttgtgga taataaaacc ctgccggagt tagtggatat
     2881 gccggtgggc gatgcccatg gttattttac taaactgaaa tttaccggcg ccaaaggcaa
     2941 aattgccgaa aaaatattaa aagaattgcg cgatcggttt cgttttttag tggatgtggg
     3001 gctgaattat ttaaccctca gccgcagcgc cgataccctt tccggcggtg aagcccaacg
     3061 cattcgccta gccagtcaaa ttggcgcggg cttggtgggt gtgatgtata ttttggatga
     3121 gccttccatc ggtttacatc agcgcgataa cgaccgcctg ctcaacacct taaaacatct
     3181 gcgcgatttg ggtaatacgg ttattgtggt ggagcatgac gaagatgcca ttcggcttgc
     3241 cgaccatatt attgacgtgg ggcctggcgc gggtgtgcac ggcgggcaaa ttatcgcgca
     3301 gggtaaatta gcgcagattg taaaagccaa aaactccctc accggtgctt atttatccgg
     3361 caaaaaaagc atcgccattc ccaaaacccg ccatgcgccc gacaagaaaa aaatgttggt
     3421 gttaagcggg gccgctggca ataatttaaa aaatgtcacg ctggaaattc ccgtcggttt
     3481 atttacctgt gtcactggcg tgtccggttc aggcaaatcc actttaatta ataatacgct
     3541 gcacccttta gcagccaccg cgctgaataa tgccaccacc ctggaggctg cgccctacaa
     3601 aagtattgag ggcttagact tatttgataa atgcgtggat atagaccaaa gccccatcgg
     3661 ccgcacgccg cgctctaatc ccgctaccta cacaggtatt ttcaccccca ttcgggattt
     3721 atttgccgcc actgcagagg cccgctcgcg gggttacaaa gccgggcgat ttagttttaa
     3781 cgtcaaaggc ggccgctgcg aggcctgcca gggcgatggc gtcgtcaaag tagaaatgca
     3841 ttttttaccg gatgtatatg tgccctgcga tacctgcaaa ggcaaacgct acaaccggga
     3901 aaccctggat gtgcattaca aaggcaaaaa tattcacgaa gttttggata tgacggtgga
     3961 ggatgcccgc gcgttttttg atgctgtgcc gagtattgcc agcaaattac aaaccctgat
     4021 ggatgtgggg ctctcctata ttcgcttagg tcaagcggca accaccctct ccggcggtga
     4081 agcccagcgg gtaaaactct cgcgggaact atccaaacgg gataccggta aaactctgta
     4141 tattttggat gaacccacca ccggcctgca ttttcacgat atagcgcaat tattagcggt
     4201 gctgcaccgc ctacgcgata acggcaatac ggtcattgtg attgaacaca atttagatgt
     4261 gattaaaacc gctgactgga ttgtggattt agggccggaa ggtggtggcg gcggcgggca
     4321 aattattgcc accggcacgc ccgaagccat ctgcaaagtg aaggcctccc acaccgggcg
     4381 atttttaaag gccgtgttgg aggcaaaaaa ataaatcccg ctctccacac cgcagccttc
     4441 catggatgga aggtttttgc cgcgtaaaaa gcccgctttc caattcgaag ttgttatttt
     4501 ctcagcacct gactcactac ctaggcaccg gcaatgaata cacagcattt cagacacgcc
     4561 gtgaatacca tcctaagtag gctcgtcaaa aagctccctc tttttgacgg gctgaaatga
     4621 tcctaaaaac cgcagttaag ctcgtagcga gggtgcctaa ggcgctgaat atgggcgctt
     4681 ttttgtcaaa atttg
//
LOCUS       DAOQGS010000323         4676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9663_length_4676_cov_5.633845, whole genome shotgun sequence.
ACCESSION   DAOQGS010000323 DAOQGS010000000
VERSION     DAOQGS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9663_length_4676_cov_5.633845"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PKZ52_12110"
     CDS             <1..1357
                     /locus_tag="PKZ52_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HNG60560.1"
                     /translation="SQDVDAAAQDPLAFSNVMYAFHYYAGTHDFNTMKNKVQTALNAG
                     LGVFVSEWGSSDVGTSYSNMAVAQQWMDFMNQKGLSWINWSLGNKDEASSILAPTAPL
                     SGPWVDADLTGPGRWLKAYFDKPTASTTPNTSSSSSSSSSSSSSSSSSSSSSSSSTGA
                     VSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTGATAAKSVSLTTSADGTR
                     ITLNWSVSNINVMQYEVFRDLDSDVNGATRLNGFGSITTNYTDSNLQAGTTYYYWIKV
                     TDTSNVVTYSQAIAGKLATTAAQTTTTTQTTSSSSSIAGNYKITSKFSGRVLDVVGNS
                     YNTSALIQQYDYNGNSNQKWGVAYDNGSNLMIYALSSYQCLDVPNGNNGTYVQQYTCS
                     HQANQNWRLLDSGNGFYQIQNVASGKCLEVTNWNGNNGTRITQSDCNNADNKLWRLDR
                     I"
     gene            complement(1624..2439)
                     /locus_tag="PKZ52_12115"
     CDS             complement(1624..2439)
                     /locus_tag="PKZ52_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60561.1"
                     /translation="MALRIFACTTAAWFCSAAVYGHEDLDLGIKDIRFDPECRPIVVI
                     QNTGERPLPATFYLAVNPAYLTVSKDAPNNRTKGQSQTSRSLRALDPHKKLLPVGGEL
                     EVRLDKPMVKNPAPLTAKLFVQGEFWEYNQKNDQLSVPIDCMPGQGTLPGIIVKPSHA
                     DLALEPAIVTARGCQVTIRIINLNHGALPVNAWSGADGIVLSIYDVQKEARLAPILLA
                     QIDPGQKLINGEVYFNESRDPGLYRYSLWQVRDDPEFSNNHQEVDLRQCNRAE"
     gene            complement(2495..3157)
                     /locus_tag="PKZ52_12120"
     CDS             complement(2495..3157)
                     /locus_tag="PKZ52_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG60562.1"
                     /translation="MYPVYGVKVNVDTAQVASPLVPLVGYETGVHLGAPDDVLQVSIA
                     AWALNIDSELIFVGDAGTTEDTAVGSARHGIELSSAWQFNPQLRWELEYSNSRAALDE
                     AIDGRRTIAGSLRHTILNEFLFTPTDRWSASLRVRYFSSYPLDDGAWAPASQLVNARL
                     HYAAEHWQLRLDLLNLLNSDDHDIEYFYASSLQGESMPYEDHHYHVFEPRSVRITWRY
                     TP"
     gene            3679..>4676
                     /locus_tag="PKZ52_12125"
     CDS             3679..>4676
                     /locus_tag="PKZ52_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60563.1"
                     /translation="MDIPPFIPPTPLQPPQNNLREGVLLLKGLMNNLLTLPADKSLWV
                     ELLRHMPLPSAQQQAILGQGGQKTSPQPSWQPLLGEAPIELVELKLPSGQKIWAATLR
                     SWFATNLAPLPPKSLLEVKPTKDGQLLMVPTKPNNLPAPQQALLGELWRKSLPQAQSP
                     KQLLQLMAPLVQPLADLPRSSRHPLQPIQQFIERQSQQVNNLISQLKIPTTETQKNQI
                     AQLTAKFINHSGVLLEPKLRSAIAQPATQPESIDEITTYDFKAQILKTLAAINQLTPH
                     TTPGDDEWPDNLWHLFLGNKEGAPSSRQNGHWLLLETLLPSLQKTLAGIQLQQTHSL"
BASE COUNT         1366 a         1268 c          956 g         1086 t
ORIGIN      
        1 gagccaagac gttgatgccg cagcacagga tcctctagct tttagcaacg tgatgtacgc
       61 cttccactac tacgcaggta cacacgactt caacaccatg aaaaacaaag tgcaaaccgc
      121 attaaatgca ggccttggcg tctttgtatc ggagtggggt tcatctgacg taggcacttc
      181 atatagcaat atggctgtgg cgcaacaatg gatggacttt atgaatcaaa aaggccttag
      241 ctggatcaac tggtctttgg gcaacaaaga tgaagcctcg tcaatccttg cccccactgc
      301 cccactgtct ggcccctggg tggatgccga cttaaccggg cctggccgtt ggttaaaagc
      361 ctactttgat aaacccactg ccagcaccac acctaacacc agctctagct ctagctctag
      421 ctctagctca agctccagtt catcaagctc gtctagctct agctcatcta gtaccggtgc
      481 cgtaagctca agctcgtcta gctctagctc gtccagctca agctcttcca gctcatcaag
      541 ctcgtctagt tcgagttcaa gttcaagctc aagctcaagc tcttctagtt cctctagcac
      601 aggtgcaaca gcggctaaat ccgtcagttt aaccaccagc gctgatggca cacgcatcac
      661 actgaattgg tctgtcagca acatcaatgt tatgcagtat gaagtgttcc gcgacctcga
      721 cagtgatgtg aacggcgcta cacgcttaaa tggttttggc tcaattacca ccaactacac
      781 cgacagtaat ttgcaagcag gtactaccta ctactactgg atcaaagtaa ccgataccag
      841 caacgtagta acctacagcc aagcgattgc gggcaagttg gccacgactg cggcacaaac
      901 aactaccacc acacaaacca ctagctcatc cagctcaatt gcgggtaact acaaaattac
      961 cagtaaattt agcggacgcg tcttagatgt tgttggcaac agttacaata caagtgcgct
     1021 aattcaacaa tatgattaca acggcaacag caatcaaaaa tggggtgtcg cttacgataa
     1081 tggcagcaac ctgatgattt atgccctgag tagctaccaa tgtttagacg tccccaacgg
     1141 caataacggc acctatgttc agcaatacac ctgcagccac caagcgaacc aaaattggag
     1201 actgttggat tcgggcaatg gtttttatca aatccaaaac gtggcaagtg gtaagtgttt
     1261 agaagtcacc aactggaatg gtaacaacgg tactcgtatc acccagagtg actgtaataa
     1321 cgcagacaac aagctctggc gccttgacag aatctaaacc tccccaaaaa acccggcgca
     1381 agccgggttt ttttatttat agctgccccc tttaacaacc gctgcttaat gactgttttc
     1441 agaattgccg caggcagaaa gcccattacg acttcaacct atccccgccc ccaaacctaa
     1501 tcaaaggggc atttccgcaa atttatttca tactagtcgc gaggcatttt cgccaggaat
     1561 gagtaagttt atgcggaaca aatggatttg cgcagatgcc aagagatttc cccgcaaagt
     1621 taattactcg gctctattac attgccgtaa atcaacctct tgatgattat tagaaaattc
     1681 tggatcgtct ctaacctgcc acaggctata ccggtacaag ccaggatcgc gggactcgtt
     1741 aaaataaacc tcgccattta tcaatttttg cccaggatct atttgcgcaa gtaaaatggg
     1801 cgcaagccta gcttcctttt gaacatcata aatgctgaga acaataccat cggcccccga
     1861 ccaggcatta acaggaaggg ccccgtgatt gaggttgata atgcgtattg tcacctggca
     1921 acctcgtgca gtgactatgg caggctctaa agccagatca gcatggctag gctttactat
     1981 aatacctggc agagttccct gccccggcat acagtcgata ggcacactta attggtcatt
     2041 tttttggttg tattcccaaa attctccctg cacgaaaagc ttagccgtca gcggtgcagg
     2101 attttttacc atgggcttat ccaagcgcac ttccagttcg ccgcccaccg gcaataattt
     2161 tttgtgcgga tcaagcgcgc gcaatgatct ggaggtctgc gattgtcctt tagtgcgatt
     2221 attgggcgcg tctttactca ccgttaaata agcgggattt acagcaagat aaaatgttgc
     2281 gggcaaaggc cgctcacccg tattttgaat aacaactatg ggccggcatt cggggtcaaa
     2341 ccttatatcc ttaatgccca aatccaaatc ctcatggccg taaacggcag cgctacaaaa
     2401 ccaagcggct gttgtacagg caaatatacg gagcgccatt ttgatttggc cagtggtgtc
     2461 atatgtcatt attaggcgac tcaaaaattg cagatcatgg ggtataacgc caagttattc
     2521 gaacagagcg cggctcaaat acatgataat ggtgatcctc atacggcata gattctcctt
     2581 gcaggcttga tgcataaaaa tactcaatat catggtcatc gctattcaac agattcaaca
     2641 gatccaacct caactgccaa tgttctgccg catagtgcaa acgagcattt accaattggc
     2701 tcgcaggcgc ccaagcgcca tcatctaatg gatagctgga aaaataacgt accctgagcg
     2761 acgcagacca ccgatcggtc ggcgtaaata gaaattcatt tagaattgta tggcgcaaag
     2821 aacctgcaat tgtgcgccgc ccgtctattg cttcatccag tgctgcgcga ctatttgaat
     2881 actctaactc ccagcgcaat tgcgggttaa attgccatgc actggataat tcaatgccgt
     2941 ggcgagcgct gcccacagct gtatcctccg tagtacccgc atcgccaaca aaaattaatt
     3001 cggaatcaat attcagcgcc caggccgcta tagacacctg caaaacatca tccggcgcac
     3061 ctaggtgcac acccgtttca tatcccacca ggggcaccaa agggcttgcc acttgagcgg
     3121 tatctacatt gaccttaacc ccatataccg gatacaaacc tgcaacaaga tcataattag
     3181 cggttgcaca tccttttgct catacagatc gggaagtatt tacgccctta ccaaccaacg
     3241 caccgggtta gacacatgca ctatctgaca aaataaagat gaatacaaaa taccaaataa
     3301 acatgcaaca taaaaattat caccggccat tttacacata aaatcaaaat aggtatcgaa
     3361 aagatcggca tgctatcacg catgcaatcc atagaaataa acaggcctaa taaaaatgaa
     3421 cctaaaaaca caaacgggtt gactacatta ttccaaaaaa aacgcaacca aaccctgccc
     3481 tccctcaaat ccaacaaata tattagagct gaggccactc ataagcgatt tccgaataat
     3541 tagtttatga gctggtatat ttacatcaaa ctctagtgca aattttgata acacgaaggt
     3601 agcgcacccc gtgtgattga atgcaaaacc atcttaattg ctgctaggct aataaggtta
     3661 aagcttggaa ccatcgcgat ggacatcccc cccttcatcc cacccacacc gctgcagccg
     3721 ccacagaaca acctgcggga aggtgtttta ctcttaaagg ggttgatgaa taacctgttg
     3781 acactgccag cagacaagag cctgtgggtg gaactgctcc gccatatgcc actgccttca
     3841 gcccaacaac aggctatcct tgggcaaggc ggccaaaaaa cgagccccca gcccagctgg
     3901 caacccctat tgggcgaagc gccaattgaa ttagtggaat taaaattacc ctctgggcaa
     3961 aaaatttggg ccgccacttt gcgctcctgg ttcgcaacaa acctcgcccc tttaccaccc
     4021 aaatcgttat tggaggttaa acccactaaa gatggccagc tcctcatggt gcccaccaag
     4081 ccaaacaatt taccggcccc gcaacaggct ttattgggtg agctctggcg caaaagcctg
     4141 ccacaggccc agtcgcccaa acaactcctg cagctcatgg cgcctttggt gcaaccactt
     4201 gctgacttgc ccaggtccag ccgccacccc ttgcagccca tacaacagtt tatcgagcga
     4261 caaagccaac aggtcaataa cctaattagc caattaaaaa tccccacaac cgaaacacaa
     4321 aaaaaccaga ttgcacaact aactgcaaaa ttcattaatc acagtggcgt gttgctagaa
     4381 cccaagttgc gcagcgccat cgctcagcct gccacgcagc cagaaagcat agatgaaatt
     4441 actacttatg attttaaggc ccaaattcta aaaaccctgg cggcgatcaa tcagttaacc
     4501 ccgcacacca cccccggtga tgatgaatgg cccgataacc tatggcattt atttttaggt
     4561 aataaagaag gcgccccgag ttcccgccaa aatggccatt ggttattgct tgaaactctt
     4621 ttgcccagcc tgcaaaaaac ccttgcgggt attcaactgc agcaaacaca cagcct
//
LOCUS       DAOQGS010000324         4671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9682_length_4671_cov_5.195407, whole genome shotgun sequence.
ACCESSION   DAOQGS010000324 DAOQGS010000000
VERSION     DAOQGS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9682_length_4671_cov_5.195407"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKZ52_12130"
     CDS             <1..501
                     /locus_tag="PKZ52_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60564.1"
                     /translation="SRTTTRHSTGAEVIAGAYILDVHVDNNGCFSSLPQPSVNGHAAG
                     VECGFLHYGPNENVHLEFTATHPNNRAVFKFDVIRGSVAVPAASAGLTETAAGSAGAY
                     TKIAGNYAHDFAAADLVGTCPLGNAAFSAQLEVYGKATNGVYRIGYDSSRWIAFALAA
                     SPTPTP"
     gene            complement(826..990)
                     /locus_tag="PKZ52_12135"
     CDS             complement(826..990)
                     /locus_tag="PKZ52_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60565.1"
                     /translation="MCPVCAAGIGTWLLAGGAGGGVVLTTTYALVKSRSQKAILKDKN
                     KQPPIKNAAS"
     gene            complement(999..1646)
                     /locus_tag="PKZ52_12140"
     CDS             complement(999..1646)
                     /locus_tag="PKZ52_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HNG60566.1"
                     /translation="MQSIVSAEEWQLQQEAFLAKEKEFTRQRDALNAQRRQLPITEIT
                     KDYRFTGADGEVSLLDLFAGHKQLIIYHFMFAKTPCTGCSMMVDNMGHSSHLHARDTA
                     RVLISLAPYPLLAEFKQRMGWQHPWFSSAGSDFNRDFNVTTDTGEMFGISVLIRDGQR
                     IFRSYFTTRRGAEYLGSNFTYLDLTPMGRQELWEQSPAWVNQTPPYQWWKKHDEY"
     gene            1787..2812
                     /locus_tag="PKZ52_12145"
     CDS             1787..2812
                     /locus_tag="PKZ52_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNG60567.1"
                     /translation="MMARPNHLGAAYMARKIIVVFFAEAVLSSAIAPLDVFTRTNSIL
                     VAQGQPAAFDVHLLTLNHAAALADNLAFVQHTQLSDFPPAPLGHDQCLIIIPAFLGNW
                     DEVCADNTALIHWLKQQYQHGSEIASLCKGSYFLAAAGLLDGKPCTSHWAVAEDLQQR
                     YPAIQLQPDAVITDQNGVYTGGGAFSSLNLIVYLIEKFCGHTLGILVAKHFSIQRDHV
                     SQAHFAMFNGLTRHNDQPIRSAQDYIQSNWQKPLTIDAIAEQVNMSKRNFIRRFKQAV
                     NLTPAEYIQRVKIEAAKKSLETGRASISDIIYTLGYNDIKTFRDTFKRITGITPQDYR
                     KKYAHEP"
     gene            2846..3472
                     /locus_tag="PKZ52_12150"
     CDS             2846..3472
                     /locus_tag="PKZ52_12150"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HNG60568.1"
                     /translation="MLMIQRHARRYLLAVIAIVLASCTQRPPLPSFNEWRSHHAQEVA
                     LLEQHLIEQDITHVLPLQQLLRSASDWSNCSEEAYAVPPPQQWDSLVMVLRLVGALRV
                     AGIMGEIEVHSGYRNSRLNSCAGGAARSAHMRSFALDFTPVNDADLTQKLCAFWRAEG
                     KNWNMGLSRYSTGRIHIDTAGFRSWGVDRDGKSTACLRAPSPHDAQRI"
     gene            complement(3605..4378)
                     /locus_tag="PKZ52_12155"
     CDS             complement(3605..4378)
                     /locus_tag="PKZ52_12155"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNG60569.1"
                     /translation="MSETASEVPEPSAPPALTAQQRDFLNYLRNPASPPPAGANPRAM
                     AIYAELVYRNLAGFINQFFPVCSQVCEHTCEEGAWQALIRAFIAQHPSQTPYFLAIAE
                     EFLQFLLSKPAALEALPAYITELAHYEWVELALDIADIPQPVPATPTQNPWDQHWQLS
                     PLAWCLAYQYPVHLIGPGNPNPAPQPSAYVVYRNAQLEVKFIALTLVSAQWLQTITPP
                     TTPRQALSGLIKGEISAGLKQQTDSLLRDWIELGVLIPV"
     gene            complement(4371..>4671)
                     /locus_tag="PKZ52_12160"
     CDS             complement(4371..>4671)
                     /locus_tag="PKZ52_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNG60570.1"
                     /translation="QFLQQLNGEAISYLHIAGHWLEEDHWRIDTHAERVDEPVWALLA
                     DVYARFGVKPTLLERDDNFPAISELTAEVGRIKQAQRGCGTEPLQTPGAGGQPHE"
BASE COUNT         1114 a         1177 c         1249 g         1131 t
ORIGIN      
        1 agcagaacca ccacgcgcca cagcaccggc gcagaagtga ttgccggagc ctatatttta
       61 gatgtgcatg tggataataa cggctgcttt agcagcttgc cgcagccgag tgtgaatggt
      121 catgccgcag gcgttgagtg cggcttttta cattacgggc caaatgaaaa cgtgcattta
      181 gaatttaccg ccacacaccc caataatcgt gcggtattta aatttgacgt aatacgcggc
      241 tcagttgctg tgcctgccgc ctctgccggt ttaactgaaa cagcggcagg ctcagcaggt
      301 gcctacacta aaatagcggg caattacgca catgattttg cagccgccga tttagtgggc
      361 acctgccccc tcggcaatgc agctttttcc gctcaattag aggtatatgg caaggccact
      421 aatggcgtgt accgcatcgg ttacgacagc tcgcggtgga tagcctttgc ccttgccgca
      481 agccccacac caacgcccta gttaactctg cggcctttta tgccgggtta atttttgtgc
      541 cgggttaatg cagcggcaag gatgccgcag caagggcgca agctgcgtta atggcagggc
      601 gaaggaggct gccctacctt aagattgcag tagattcgcg gtgcagcagt gatgaataca
      661 ggttattgca gacagtcaaa aagaggggcc tttttgacga gcctacacgg atgtatttac
      721 ggcgcctccg caataacctg tatttatcac ggcggatgga gcattgaggg ctcgccgctc
      781 gcctgacggt ttttagccgg tgcagatatt ttgattcacc agcgcttagc tagccgcgtt
      841 tttaatcggc ggctgtttat ttttatcttt taaaatagct ttttggctgc gggattttac
      901 taacgcataa gtcgtcgtta acaccacacc gccgcctgca ccacctgcta ataaccaagt
      961 gccaataccc gctgcacata cagggcacat aattcacctc aatactcgtc gtgttttttc
     1021 caccattgat agggtggggt ttggttaacc caggcggggc tttgttccca caactcctgg
     1081 cggcccatgg gggtcaaatc caaataggta aaattactgc ctaggtattc agcccctcgg
     1141 cgagtggtga aataactgcg aaaaatccgc tgtccatcgc gaattaatac actgatgcca
     1201 aacatttccc ccgtatcggt ggtgacatta aaatcccggt taaaatcgct gccggctgag
     1261 gaaaaccaag ggtgctgcca gcccatgcgc tgcttaaatt ctgccaataa tggatagggt
     1321 gctaaggata ttaatacccg cgctgtgtcg cgcgcgtgca ggtgcgacga gtggcccata
     1381 ttatccacca tcatagagca accggtgcag ggcgttttag caaacataaa atgataaata
     1441 atcagctgtt tgtggccggc aaataaatcc aataatgaaa cctcgccatc ggcgccagta
     1501 aagcggtaat ccttggtaat ttcggtaata ggtaattgtc ggcgctgggc atttaatgcg
     1561 tcgcgctggc gggtaaactc tttttctttg gctaaaaaag cctcctgctg tagttgccat
     1621 tcttctgcac taacaataga ttgcataact gcctccaatt aatatgtgtg agcccaattt
     1681 aacccggcgg ctttttatgc cgggttaata gtatgctgca tgtttaacgg ccatgcgctg
     1741 tgaataaatg ccatccatag ggtggttttg cgccaagggc tccactatga tggctcgccc
     1801 taatcacctg ggagcagcct atatggcccg caaaatcatc gtagtatttt ttgccgaagc
     1861 agttttatcc tcggccattg ccccattaga cgtatttacc cgcaccaatt ctattttggt
     1921 agcacagggc caaccggcgg cgtttgatgt tcatctactc acgctgaatc acgcggccgc
     1981 gcttgcagat aacctagcct ttgtacaaca cacccagttg agcgactttc cacctgcacc
     2041 cttagggcac gatcagtgtt taattattat tccagcgttt ttaggcaact gggacgaggt
     2101 gtgcgccgac aatacggcgc tcattcattg gctaaaacag caataccaac acggcagtga
     2161 aattgcgagc ctgtgtaaag gcagttattt tttggcggcg gcgggtttat tagatggcaa
     2221 gccctgcaca tcccactggg cagtagcaga ggatttacag cagcgctatc cggcgattca
     2281 attacaaccc gacgcggtaa taaccgatca aaatggtgta tatacgggcg gcggggcatt
     2341 ttcatctttg aatttaattg tgtatttaat tgaaaaattt tgcggccata cgcttggcat
     2401 cttggtggcc aaacattttt ccatccagcg ggaccatgtc agtcaggccc attttgctat
     2461 gtttaatggc cttactcggc acaatgacca acccattcgc agcgcccagg attatattca
     2521 aagtaactgg caaaagcctt taactattga tgccattgcc gagcaggtga atatgagcaa
     2581 gcgcaatttt attcggcgct ttaaacaggc cgttaacctc accccagcag aatacattca
     2641 aagagtaaaa atagaagcgg caaaaaaatc cttagaaacc ggcagagcga gtattagcga
     2701 tattatttac actttaggct acaacgatat caaaaccttt cgcgatacgt ttaaacgcat
     2761 aaccggtatt acaccgcagg actaccgcaa gaaatatgca cacgaaccct aagcctgcgc
     2821 ggccagccat aaaagcgcaa tcagtatgct aatgatccaa cgccatgcac ggcgctattt
     2881 attagcggta attgccattg tgcttgcgtc atgcacacaa cggccgccat tgccgtcgtt
     2941 taatgaatgg cgcagccacc atgcgcaaga agttgccctg ctcgaacaac acctaataga
     3001 gcaggacatt acccacgtgc tgccgctaca gcaattattg cgttcggcct ccgattggtc
     3061 gaactgctct gaagaggcct acgcagtgcc gccgccacag cagtgggatt cgctggttat
     3121 ggtgttgcga ctggtgggtg cgctgcgggt tgcgggaatt atgggtgaaa ttgaagtgca
     3181 ctcggggtat cgcaattcca gattaaacag ctgtgctggc ggcgccgccc gcagcgcgca
     3241 tatgcgcagt tttgccctgg atttcacgcc tgtgaacgac gccgatctca cgcaaaagct
     3301 gtgcgcgttt tggcgcgccg agggcaaaaa ttggaacatg ggcctcagcc gttattccac
     3361 tgggcggatt catatagata ccgcagggtt tcgctcttgg ggtgttgatc gcgatggaaa
     3421 atcaacggca tgcctgaggg cgccatcgcc gcatgatgct cagaggattt agctgaaaag
     3481 tctcaaagcc aaggaatata aatttagcta attaatacac cactgccttt tttaagcagg
     3541 ttcgccgcaa taacaaatta cctaaaattt taatccgcgc ccacgcaata gctgcaccta
     3601 aacattaaac gggaattaat acgcctaatt caatccagtc gcgcaataag ctatcagttt
     3661 gttgtttaag gcctgcggat atttcgcctt tgattaagcc gctgagggct tggcgcgggg
     3721 tggttggtgg ggtgatggtt tgcagccatt gggcgcttac cagtgtgagg gcgataaatt
     3781 tcacctccaa ctgggcattg cggtacacca cataggcgct gggttggggg gctgggttgg
     3841 ggttgccggg gccgattaga tgcaccgggt attgataggc taaacaccag gccagggggg
     3901 agagttgcca gtgctgatcc caagggttct gggttggggt ggcgggcacc ggctggggaa
     3961 tatcagctat atccagggcc agctctaccc actcgtagtg ggcaagctcc gtgatatagg
     4021 cgggcaaggc ttctagggcg gccggtttac tgagtaaaaa ctgcaggaat tcttcggcaa
     4081 tggctaaaaa gtacggggtt tggctggggt gctgggcgat aaaagcgcga attagcgcct
     4141 gccaggcgcc ttcttcgcag gtgtgctcac aaacttggct gcagacgggg aagaactgat
     4201 tgataaaccc ggccaggttg cggtacacca actcggcgta gattgccata gcccgcgggt
     4261 tggcgcccgc aggtggcggg ctggccgggt tgcgcaggta gtttaaaaaa tcgcgctgct
     4321 gggcggttaa tgctggtgga gcggagggtt cgggcacttc gctggcggtc tcactcatgg
     4381 ggctgccctc ctgcgccggg ggtttgcaaa ggctcggtac cgcagccgcg ctgcgcctgt
     4441 ttgatgcggc caacttcggc agtgagttca ctgatggccg ggaagttatc gtcccgctcc
     4501 aaaagggtgg gtttaacgcc gaagcgtgcg tagacgtcag cgagcaaagc ccacaccggc
     4561 tcatccacgc gctcggcgtg ggtgtcgatg cgccagtggt cctcttccag ccagtgcccg
     4621 gcgatatgca gataactgat ggcctcgcca tttaactgct gcaaaaactg c
//
LOCUS       DAOQGS010000325         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9717_length_4661_cov_4.822623, whole genome shotgun sequence.
ACCESSION   DAOQGS010000325 DAOQGS010000000
VERSION     DAOQGS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_4661_cov_4.822623"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="tilS"
                     /locus_tag="PKZ52_12165"
     CDS             <1..526
                     /gene="tilS"
                     /locus_tag="PKZ52_12165"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNG60571.1"
                     /translation="LWFSQLHLLPPSQAVLGQMELLMNSRLDSEACVNWGQWQLRRFT
                     GRLYVMPQLNPLAPDWQGRWHTQTPALMPNGSYLSAQPYELGLAPGAYTLRLRQGGER
                     SHPEQRAHSQTLKKLLQEAGVEPWLRNQLPLVYQGEQLVAVGDLWLERGFSVQQGVKL
                     SWNFSAHAHLLEHS"
     gene            526..1305
                     /locus_tag="PKZ52_12170"
     CDS             526..1305
                     /locus_tag="PKZ52_12170"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNG60572.1"
                     /translation="MRDEFVHKGYTVVRNFLTPDQVAALNKPLRQFHSTWLAEHADVY
                     ARGAVNSAYITSTRFRQNPARQELFTFLASHRFMVEVEKVVGDSACFMNTQLFFDPHN
                     TAQNNYWHRDSQYHLNLREQQESLRGPQVVHFRIPLVDERGIELVPGSHKRWDTAEEL
                     DVRLERNHRQRHDSLSSGAAIALKAGDLCVFSAAMIHRGLYGLNRFALDILMCDPAPE
                     LLQFVDNDCLPTQQELTALEHPAAFLRTLTQLNACGGASLP"
     gene            complement(1329..1589)
                     /locus_tag="PKZ52_12175"
     CDS             complement(1329..1589)
                     /locus_tag="PKZ52_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60573.1"
                     /translation="MKTLLLAPFLLSLLAHASFAAEASCEAKAAEKKLSGAAQTSFIK
                     KCEKDAKASTAKVECEAKAVEKKLFGAAKNSFTKKCIADAGQ"
     gene            1588..1764
                     /locus_tag="PKZ52_12180"
     CDS             1588..1764
                     /locus_tag="PKZ52_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60574.1"
                     /translation="MVVPALVVVAATIVAGYKSCQFGGSLSVSGFNIAICVQPTQHIP
                     AVCLAHTLCRISFY"
     gene            2675..>4661
                     /locus_tag="PKZ52_12185"
     CDS             2675..>4661
                     /locus_tag="PKZ52_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60575.1"
                     /translation="MGLRSGEVPVSVKQNYLLRVTTYGLIFLLHLSLSWGQFSFGFWL
                     LIALQGLVYPHLVYFWIPNNAAEHRLLLLDAAAYGVHLALWGFNPYLVAFFTAAMGVT
                     YLSVGGRPMLIKALLFHGLGLALGGLGTGWRFLPQIPLLTLAIVATGFTLYLLNLGSL
                     VYATNSKLRTARKQALEQQEELVLTHRLVATVNGSLDMDVILQTIMEAVQRYYPFETL
                     YMLPYSEDKTAFEITGIYGDSVTDLERTQFMRMQFDLEKDADSIFVRGLTRNKIVYLP
                     RMSPAAAATSAPIDQHLYAIKSAKSLLYVPVCFNNEVIAGAAFLNYEVPFDLEEKDFA
                     RISQFLMHAASAVRNLYLIRELKAASAAANDARVRAEQSEEAKGRFLANMSHEIRTPL
                     TAVLGYADLLTDKNLSVKDRDKFTQIILQSGQHLLAMINDILDIAKIEAGKMSIETLP
                     CSLVETLYPLDALMRLRALEKGLVFHLELNYPIPKIVHTDATRLKQILLNLLNNAVKF
                     TQEGSVRLSVARVDSQLVFEVRDTGIGIDEQQAKQLFSAFTQADSSTTRLYGGTGLGL
                     FVSKNIAQLLGGDISLHSKVGEGSTFTVRIQCPVGDEFIADEASFARLWEEQSRQHEA
                     QQLPQLHGKILVAEDNLENQRLISHVLQQLNVRFDMAG"
BASE COUNT         1046 a         1174 c         1212 g         1229 t
ORIGIN      
        1 cctgtggttt agccaattgc acctactgcc gcccagccag gcggtgctgg ggcagatgga
       61 actgttaatg aatagccgct tggacagcga ggcctgcgta aactgggggc agtggcaatt
      121 gcgccgcttt accgggcggc tttatgtaat gccgcaactc aaccccttag cgcctgattg
      181 gcaggggcgc tggcataccc aaacacccgc gctaatgccc aacggcagtt atttaagcgc
      241 gcagccttac gagctgggcc ttgcccccgg tgcatacacc ctgcgcctgc gccagggcgg
      301 cgagcgcagc caccccgagc agcgcgccca ttcccaaacc ctgaaaaaac tcctacaaga
      361 ggcaggtgtt gagccgtggc tgcgcaatca attgcccttg gtgtatcagg gcgagcaatt
      421 agtggcggtg ggggatttgt ggctggagcg gggttttagc gtacaacagg gcgttaaact
      481 cagctggaat ttttcggctc atgcgcattt attggagcat tcttgatgcg ggacgaattt
      541 gtgcacaagg gttacacggt ggtgcgcaat tttttaacgc ctgatcaagt tgcggcatta
      601 aataaaccgc tgcggcaatt tcacagtact tggcttgctg aacacgccga tgtgtacgcg
      661 cgcggcgccg ttaattcagc ctatataacc agcacgcggt ttcgacaaaa tcccgctcgg
      721 caggagcttt ttaccttttt ggcatcgcac agatttatgg ttgaggttga aaaggtagtg
      781 ggcgacagcg cctgttttat gaatacacaa ttatttttcg acccccacaa tactgcgcaa
      841 aataattatt ggcaccggga cagccaatat catttaaatc tgagggaaca acaggaatct
      901 ttgcgtggac cccaggtggt acattttcgt attccactgg tggatgagcg gggtattgaa
      961 ctggtgcctg gaagccacaa gcgttgggac acagccgagg aattagacgt gcgcttggag
     1021 cgcaatcacc gccagcgcca tgattcttta agttccggcg cggccatagc acttaaggcc
     1081 ggggatttgt gcgtattttc cgccgccatg atccaccggg gtttatacgg tttaaatcgt
     1141 tttgcgctgg atattttaat gtgcgatccg gcgcccgaac tgctgcaatt tgtggacaac
     1201 gactgtttgc ccactcagca ggaattaacc gctttggaac atccggctgc gtttttacgc
     1261 acgctcaccc aactaaacgc atgcggtggc gcatccttgc cgtaaagttg cgcccgtaaa
     1321 tcctgatttt attgcccagc atcagcaata cattttttag tgaagctatt tttcgctgcg
     1381 ccaaacaatt ttttctctac agctttagcc tcacactcca ccttggctgt gctggctttg
     1441 gcgtcttttt cacatttttt aataaaacta gtttgcgctg cgccggagag ttttttctcg
     1501 gcggcttttg cttcgcagct ggcttcggcg gcaaagcttg catgggcaag cagcgataat
     1561 aaaaaaggag ctaagagtaa tgttttcatg gtggtgcccg cgttggttgt ggtggcggcc
     1621 actatagtag ccggttataa atcctgtcaa tttggcggct cgttaagcgt atctggtttt
     1681 aatatcgcta tttgtgtgca gcctacgcaa cacatccccg ccgtctgcct ggcccataca
     1741 ctctgccgta ttagctttta ctaactgcca tcggcagctt tatttgcgcc acatcaaact
     1801 tggcgggaaa tcgcttgatc ggcatcaaaa gctatttttc gccatgcgcc aaacttaatt
     1861 catagcaaga aattatgttc cattgagata agtcgaggtg tttatgtatc tgccagctgt
     1921 tgttgttacc gttacattaa ttgccacttt ggtattgttg ggggtgggtg gcgctgggtt
     1981 aattattgct gataataaat gcactgggct gcgtcatcat taatcagcac ctgcacgcgg
     2041 gcgcgttctt ggcagtctgt taggcatacg gtgctgagct tacagagcaa atacgtccat
     2101 ataagtgtgt gattgcagaa atgtgagacg acccaatgga cggggtaggt agaatttcgt
     2161 cgggaatgaa atcgacgatc ctgtggatgg taacgtcatt tagttaagag ttttgtgccg
     2221 tcgtttattt gaatgtgtac ttatccctaa aacctccccg tcagaaaggg gagggagcaa
     2281 aacgcctttg ttgtgcggtt ttttgctcct ccccctttcc aagggggagg ctggaagggg
     2341 tcgggtaaac agcaaggcac tatgaaaacc aggtcaaaaa cctaaccgag gggcatcgcc
     2401 gttgtatccc taattcgcaa cccgctgttg cattgatcat tcgttacgac tccaaactgc
     2461 ctcttaccgt cggcgggcat ctgcccactc aattgcatta atttttataa atacgcgcac
     2521 cggcacaact gcccaggggg cgctgtggca caatggcggc cccgcctttg atccatcagc
     2581 agtactccca tggcccattt atgtacacgg caatcgcccc ttggggttaa tccactatac
     2641 ttcttgaata cctgttatta ggaagataga tcacatgggg ttgcgatccg gcgaggtgcc
     2701 tgtcagcgtc aaacaaaact acctgctgag ggttactacc tacggcctga tatttttgtt
     2761 gcacctgagt ttaagttggg ggcaattttc tttcggcttt tggctgttga tcgccctaca
     2821 gggtttggta tacccccatt tagtctattt ctggattccc aataacgccg ccgagcatcg
     2881 cttgctgctg ctcgatgccg ctgcctacgg cgttcattta gccctttggg gctttaatcc
     2941 ttatttggtc gctttcttca ccgccgccat gggcgtcacc tatctatcgg taggcgggcg
     3001 gccaatgctg atcaaggcgt tgttgtttca cggcttgggc ttggccctgg ggggactggg
     3061 cacgggctgg cggtttttgc cgcaaatacc cctgcttacg ctcgccatag tcgctacagg
     3121 gtttactctg tatctactca atctcggcag cttggtgtac gccactaatt ccaaattgcg
     3181 caccgcccgc aagcaagcgc tggagcagca ggaagaatta gtgctcaccc atcgcttggt
     3241 ggccacagta aatggctctt tggatatgga tgtgattttg caaaccatca tggaagcggt
     3301 gcagcgctat tacccgtttg aaacccttta tatgctgccc tacagcgaag ataaaaccgc
     3361 ttttgagatc accgggattt acggcgattc agtgacggac ttggagcgca cgcaatttat
     3421 gcgcatgcag tttgatttag aaaaagacgc cgacagtatt tttgtgcgcg gccttacccg
     3481 caataaaatt gtttatctgc cccgcatgtc gccggcggct gcggccacct cagcgcccat
     3541 tgatcagcac ctgtacgcca tcaaatcggc caagtcctta ctctatgtgc ccgtttgttt
     3601 taataacgaa gtgatcgcag gtgcggcctt tttaaattac gaggtgccgt ttgatttaga
     3661 agagaaggac tttgcccgca tcagccaatt cttaatgcat gccgccagcg ccgtgcgcaa
     3721 cctgtattta attcgtgagc tcaaagcggc ctccgccgcc gccaacgatg cgcgggtgcg
     3781 cgccgagcaa tcggaagaag ctaaggggcg tttcctggct aatatgagcc acgaaattcg
     3841 cacgcctcta acggcggtgc tcggttatgc cgatttgctt accgacaaaa acctctcggt
     3901 taaagatcgc gataaattca cccagattat cctgcaaagc ggccaacacc tgctggccat
     3961 gatcaacgat attttggaca ttgccaaaat tgaagcgggc aaaatgtcca ttgaaacact
     4021 gccctgttct ctggtggaga cactctaccc tttggacgcc ctcatgcgct tgcgcgcctt
     4081 ggaaaagggc ttggtgttcc atcttgagct gaattatccc atccctaaaa ttgttcacac
     4141 cgatgccacc cgcctaaaac aaattctgct taatttgctc aataatgccg ttaaatttac
     4201 ccaggaggga tcggtgcgct tgtcggtggc gcgagtggat tcgcagctgg tctttgaagt
     4261 gcgcgatacg ggtataggta ttgatgagca gcaagccaaa caattatttt cggcttttac
     4321 tcaggcggat agctctacca cgcggctgta tggcggtact gggctcggtt tatttgtgtc
     4381 aaaaaatatc gcccaattat tagggggcga catcagtttg cattctaaag taggcgaggg
     4441 cagtacgttt acggtacgta tccagtgccc ggtgggggat gaatttattg ccgacgaagc
     4501 aagctttgcc cgcctctggg aggagcagag ccgccagcac gaagcgcaac aattaccgca
     4561 gttgcacggc aaaatattgg tggccgaaga taacctggaa aatcagcgtt taattagcca
     4621 tgtgttgcag cagctcaatg tgcgttttga tatggcaggc a
//
LOCUS       DAOQGS010000326         4658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9723_length_4658_cov_4.805779, whole genome shotgun sequence.
ACCESSION   DAOQGS010000326 DAOQGS010000000
VERSION     DAOQGS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9723_length_4658_cov_4.805779"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1249)
                     /locus_tag="PKZ52_12190"
     CDS             complement(83..1249)
                     /locus_tag="PKZ52_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNG60576.1"
                     /translation="MQRFAIAPRPNWKLKAREYGFNFHTLMGEPYWREDAYYSFTLAQ
                     IENDIEAPTVELHQMCLHAVSQVVASERLMQQFCIPQMHWNLIAESWRKQDPSLYSRL
                     DLAYTGQGPAKLLENNADTPTSLYETGFWQWLWLEDQLQAGLLPAGADQYNLLQEKLI
                     ERFRQLKCHFPDTLLHLSCCQDSSEDRGTVQYLADCALEAGIANEFVFIEDIGLSEHG
                     PFTDLNNGEIRWLFKLYPWEFMLREAFAEHIAKSKTQWLEPPWKALVSNKAILPLLWE
                     LFPNHPNLLPAYFDCDPRAHFSGRYVKKPIFAREGANISIWQGDAAILASDGPYGEEG
                     YILQGYTALPEFNGRHALVGSWLVDGEACGLSIREDSSPITQNFSAYLPHIIAP"
     gene            complement(1249..1794)
                     /locus_tag="PKZ52_12195"
     CDS             complement(1249..1794)
                     /locus_tag="PKZ52_12195"
                     /inference="COORDINATES: protein motif:HMM:NF018408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HNG60577.1"
                     /translation="MTVLFAGCESNEQQADIFKTLEECKTTYPDLSAQCEMAFNEALK
                     KAEDTAPKYKTLDDCIIDFGPERCTPYKSADGNNWFMPAMAGFMLARALDDNRRYNTA
                     PMFTSSINSSPYYNRWTTVDGNSYGDYRNRQVRTNADAFRPQPRITKTIERGGFGSKA
                     AAASSWGSSSTKSSSRSSWGG"
     gene            complement(1885..2301)
                     /locus_tag="PKZ52_12200"
     CDS             complement(1885..2301)
                     /locus_tag="PKZ52_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNG60578.1"
                     /translation="MEFFHTSLQGLVNFASYFFASITLLIIFSFVYVAITPHDEWKLV
                     KQEKNIAAATGLAGALIGFAIALGSVVSNSVHLLDFVLWGVIALIAQLIAFALVRFVF
                     MPELIQRIKDNEMSAGVILGATSIAVGVLNAACLTY"
     gene            complement(2411..3235)
                     /locus_tag="PKZ52_12205"
     CDS             complement(2411..3235)
                     /locus_tag="PKZ52_12205"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60579.1"
                     /translation="MDAQEVTKILDEYYEELTGILGRFTKTRDGIHIDQKDGYRLREI
                     ANELVDFVADHIPNSKHHAALIANYYNQGISNWLSSSSYASVEEIRGSVKSLKIRIER
                     NPKIFEEESGEIVVDKGEKIKFDGIETIANRFHAVVMQLRQRYDSRTTIDVNDEYDVQ
                     DLFHSLLRLFFNDIRAEEWNPSYAGGSSKSDFLLPEIGTVVEIKKTRKSMTTKQLGEQ
                     LIVDIAKYKKHPQCARLICFIYDPEGRVNNPRGIENDLSNCDSDLDVRTIIVPKHI"
     gene            complement(3364..4005)
                     /locus_tag="PKZ52_12210"
     CDS             complement(3364..4005)
                     /locus_tag="PKZ52_12210"
                     /inference="COORDINATES: protein motif:HMM:NF022144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HNG60580.1"
                     /translation="MFGSLFGKKDSHPSLPKAPEVLGLFLGGSVKIDPLKLRLVEPDL
                     IIEGAASDQIIQAVGVIDLDPQTQILRFYTDDDGFIQVLQSGGKGDEYVADVKLWYFY
                     ETRSVGNDWEQVLKHNISLPEYSLDGHTFTRSWQAAGSDNPPVAMTEKTYHLEGSVSE
                     TDQFLMLYERQANENLAEYLTLAGEESLCAGTFERNLVICTGFDLSAADFTAN"
     gene            complement(4154..>4658)
                     /locus_tag="PKZ52_12215"
     CDS             complement(4154..>4658)
                     /locus_tag="PKZ52_12215"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNG60581.1"
                     /translation="KLASLTSSVTEYENHARAAAAKGDQALALDCAKKVAELRAQVEA
                     EQQLVSQFTQSESTLRKNISQAKNNLARMEQQVDMVKATESVQKAQVSVSSRHLGANN
                     KMKTATESLARIQEKQKLRQAEFEAAAELASDESGDDLQKRLRDAGISGPSTSADDEL
                     ARILGKQ"
BASE COUNT         1193 a         1069 c         1067 g         1329 t
ORIGIN      
        1 gctaaatcct cctgaatttg ccgcccgcgc ggaagaaagg agtaaagtgt gctgagtagt
       61 tactaagtat tttaatgtgc tgttagggcg caattatgtg gggtaaatag gcggagaagt
      121 tttgtgtaat aggtgatgag tcctctcgta tggaaagccc gcaggcttcg ccatcgacta
      181 accaagagcc tactaatgca tggcggccat taaattccgg caaggctgta taaccttgca
      241 gaatataacc ttcctcacca taggggccgt cagaggctaa aatggcggcg tctccttgcc
      301 aaatacttat attcgcgcct tcgcgggcga aaatgggttt tttaacatat ctgccactaa
      361 aatgtgctct agggtcgcag tcaaaatacg ccggtaataa attaggatga ttgggaaaca
      421 attcccataa caggggtaaa atggctttat tggaaactaa cgctttccac ggaggctcta
      481 accattgtgt tttactcttt gcaatgtgct cggcaaatgc ctcgcgcagc ataaattccc
      541 aggggtagag tttaaataac cagcggattt ctccattatt aagatcggta aatgggccgt
      601 gttcgctaag gcctatatct tctataaaga caaattcatt agctataccc gcctcgagtg
      661 cgcaatccgc caagtattgc acagtgccac gatcctcgct ggaatcttgg cagcagctta
      721 aatgcaagag tgtatcggga aaatgacatt ttaattgtcg aaaacgctcg atgagttttt
      781 cctgcagtaa attatattga tcggctcctg ccggtaataa tccggcttgc aattgatctt
      841 ccagccaaag ccactgccaa aaaccggttt catagaggct tgtgggtgtg tctgcgttat
      901 tttcgagtaa ttttgcaggg ccctggcctg tataggctaa atccaagcgg gagtagaggc
      961 ttgggtcctg tttgcgccag gattcggcaa ttaaattcca gtgcatctgt ggaatacaaa
     1021 attgctgcat taggcgctca ctggccacaa cctgacttac cgcatgcaaa cacatttgat
     1081 gtaattcaac tgtcggcgct tctatatcgt tttcaatttg cgccagtgta aaggagtaat
     1141 atgcgtcttc ccgccaatag ggctccccca taagagtgtg aaaattaaag ccatattcac
     1201 gggctttgag tttccagttg ggcctggggg caatggcgaa acgctgcatt agccgcccca
     1261 actagagcgc gaggaagatt ttgtactgct gctgccccaa ctgctggccg ccgctgcttt
     1321 agagccaaat ccgccgcgtt caatcgtttt agttatcctg ggttgcgggc ggaatgcatc
     1381 ggcatttgtg cgtacttggc gattgcgata atcgccgtaa gaattgccat ccaccgtagt
     1441 ccagcggttg tagtaggggg atgagttaat ggaggaggta aacatgggcg cggtgttata
     1501 cctgcggtta tcatccagcg cacgggcgag cataaaccca gccatcgcag gcataaacca
     1561 attattgccg tcggcacttt tgtacggtgt gcagcgctct gggccaaagt caataataca
     1621 atcatccagc gttttatatt taggcgctgt gtcctcggcc tttttaagtg cttcgttaaa
     1681 tgccatttcg cattgggcgc ttagatcggg gtaggtggtt ttacactctt ccagggtctt
     1741 aaaaatatcg gcctgttgtt cattggactc acagccggca aataataccg tcatacacaa
     1801 agccagcggc tttaaggcaa aaactcctga caatttgcgc atgcgcgaaa ggtttatcga
     1861 ttggctcctt ttcataactc acctctaata agtcaggcag gcggcgttga gtacacctac
     1921 cgcaatggaa gtggcgccga gaataacccc tgcggacatt tcattatctt taatacgctg
     1981 aattaactca ggcataaaca caaagcgcac cagagcaaaa gcaattaatt gtgcgatgag
     2041 tgcaataaca ccccagagga caaaatctaa taaatgaaca gaattactca caacactgcc
     2101 cagtgcgatt gcaaacccaa taagcgctcc ggctaaaccg gttgcggcgg cgatattttt
     2161 ctcctgtttg actaacttcc actcgtcgtg gggtgtgatg gcaacgtaaa caaaactaaa
     2221 gataatgagc agcgtgatgc tggcaaaaaa gtaactggca aaattcacca ggccttgcag
     2281 tgaggtgtga aagaattcca tatagagtct ctttgattgt tgggcttgtt ttgatgttgc
     2341 cgcttaacgc cgcgctatgg ggcttgccgt agctcaatat aggcaagtat cgagcagcgt
     2401 tttgttaaat ttatatatgc ttgggcacga tgattgttct aacatcgaga tcactgtcac
     2461 agttacttaa atcgttctct atgcctcttg gattattaac tcttccttcc ggatcatata
     2521 taaaacatat cagtcgtgca cactgaggat gctttttata ttttgctatg tcaactatga
     2581 gctgttctcc taattgtttg gttgtcatag atttacgtgt tttctttatt tccacaaccg
     2641 tacctatttc gggcaacagg aaatcgcttt ttgatgaccc gcctgcatag cttggattcc
     2701 attcttcagc tcgaatatca ttaaagaaca accttagtaa gctgtgaaac aggtcttgga
     2761 catcatattc atcatttacg tcaatcgttg ttcttgagtc gtatcgttgt cgtaactgca
     2821 taacaacagc atgaaatctg tttgcaattg tctcaatacc gtcaaactta atcttctcac
     2881 ccttgtcaac tacgatttca ccactttctt cctcgaagat ctttgggttt ctttcgattc
     2941 taatctttag agacttaaca gaacctcgta tttcttcaac gctggcatag gaagaactac
     3001 taagccaatt gctaattccc tggttgtaat agttagcgat taacgcagca tggtgtttgg
     3061 aatttgggat atggtctgca acaaagtcta ccagctcatt tgctatttct cttaaccgat
     3121 agccatcttt ctgatcaata tgaataccat ctcgggtttt agtgaatctc cccaatatgc
     3181 cagtgagctc ctcataatat tcatcaagta tttttgtaac ttcttgtgcg tccataagac
     3241 tccaaaaaat ttaacagcca gttattgacc tggcggcaaa tgccgggtta ataactggcg
     3301 ttaagcgata gcacaaacag ctgccgacca gggcaaaaag aattattccc tggggcgttg
     3361 catttaattg gcagtgaagt ccgccgcact taaatcaaac cctgtgcaaa tcactaggtt
     3421 gcgctcaaag gtgcctgcgc acagggattc ttcaccggca agggttaagt attctgcaag
     3481 attttcattg gcttgtcgct cgtacagcat caaaaattgg tcggtttccg ataccgagcc
     3541 ctccagatga taggtttttt ccgtcatagc aacaggtggg ttatcgctac cggccgcctg
     3601 ccaactgcgg gtgaaggtat ggccatcaag gctatactcg ggcaggctga tattgtgttt
     3661 aagcacctgt tcccagtcat tgcctacgga gcgggtttca tagaagtacc acagctttac
     3721 atcggcaaca tattcatcgc ccttgccgcc actttgtaat acctgaataa atccgtcgtc
     3781 gtcggtataa aaacgcaaaa tttgggtctg agggtctaaa tcaattacgc ctacggcctg
     3841 gataatttga tcgctggcag cgccttcaat aattagatca ggctccacta agcgcaattt
     3901 caacggatca attttaacgc taccgcctaa aaataggccg agtacttcag gagccttggg
     3961 taggctggga tgtgagtctt tttttccaaa caatgagcca aacatacagc aggtctccgt
     4021 taaattattg agcgagcgta atagaaaaac ccgcacagca agccttgttt aactgcagtg
     4081 gcgggttatt gattcacgat taagcttgtg ttttaaatgg gcagttacac agaggtaacc
     4141 gcccatttaa aggttactgt ttacctaaaa tccgcgctag ctcatcatca gcagatgttg
     4201 atggcccgct gatacccgcg tcgcgcaggc gtttttgtaa gtcatcgccg ctttcatcgg
     4261 aggctaattc cgcagctgct tcaaattccg cctggcgcaa tttttgtttt tcctgaatgc
     4321 gcgctaagga ttcggtggca gttttcatct tattgttagc gcctaggtgg cgagaggaaa
     4381 ccgaaacctg ggctttttgt acagactcag tggcctttac catatcaacc tgctgctcca
     4441 tacgcgctaa attgttttta gcttggctga tgtttttgcg cagggttgat tccgattgtg
     4501 taaattgact taccaattgt tgctcggctt ctacctgtgc gcgcaattca gcgacttttt
     4561 tggcgcaatc caatgctaag gcttgatcgc ctttagccgc tgcagcgcgg gcatggtttt
     4621 catattcagt tacggaactg gtcaggctgg caagtttt
//
LOCUS       DAOQGS010000327         4633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9790_length_4633_cov_5.216033, whole genome shotgun sequence.
ACCESSION   DAOQGS010000327 DAOQGS010000000
VERSION     DAOQGS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9790_length_4633_cov_5.216033"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..244)
                     /locus_tag="PKZ52_12220"
                     /pseudo
     CDS             complement(170..244)
                     /locus_tag="PKZ52_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
     gene            498..1763
                     /locus_tag="PKZ52_12225"
     CDS             498..1763
                     /locus_tag="PKZ52_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNG60582.1"
                     /translation="MKLHWQILIAIGLAFITGLLLPKDSQVLGVSLYSLLGFFGAAFL
                     QGLKMLVIPLVISSVICGVAGIGSNRLGRMGWLTAATFIGFTASAVLVGLLMVNLFSP
                     GTHNGKPASDLLNLVDTQQVAAQVAKVAERDGGDLAGVFLRMIPSNIVAAASDDGQML
                     GVIMFSLLFGYFLLQSAEPGRSAQLSFWRGLNDTMMRMTLFVMKFAPIGVYGLITQTV
                     MNTGLKSLSVMGVFFITVAAGLILHCFGTLALYLKVFGRVNPLKHYKAMFPAMLTAFS
                     TASSNASLPVNIKCLEENAKVSHKTTSFVLPLGASLNMDGTALYECVAVIFIAQAYGV
                     DLTHSQQVMVVLMALLTSFGIAGIPAASLVAISVILGSLGLPIEAIALLMVTDRILDM
                     MRTAVNVFSDSCCAVVVARVEGEKGVLVD"
     gene            1900..>4633
                     /locus_tag="PKZ52_12230"
     CDS             1900..>4633
                     /locus_tag="PKZ52_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNG60583.1"
                     /translation="MKWFRDLPIVRKQMLMLMGVALLPILILGLLSYSTAKQELSHSA
                     TEALIAVRDLKTAAINRHFDQLRGQITSMALSEDVQIALVELTQGFNSLNSGTPPNSS
                     GLAAYYQSQFAAEYQKRSQKAIATQPILAALSPAGLWLQTEYIANNANPLGSKHLLNS
                     GADETPYNRTHAKLHPKLRHELEAFGYYDIFIVDNASGNVVYTVFKELDFATNLTTGS
                     WAKTDLARAFGQAKTLPQGKTSLTDFAPYIPSYEAPAAFLATPIYIQGKALGTLIVQV
                     PIEPINAIMQERSGMGKTGETYLVGPDYLMRSDSFLDPTHHSVSASFAAPETGSVKTE
                     AIKKALSGQTSAELLIDYNGNPVLSAFAPLKIADFSWVIAAEIDQAEALKSVYSLRTL
                     TLIIGVILLAAVAVLALFSARMVSQPITSMEHIISRVQKEGNFRLSLNNRDQDEIGKT
                     CRSVDKLVLDTGRVIQLVNLHLQQLVRGETVNAITEVFPGDLGLLAQGVNSTVEAIDS
                     ARKEQARQAVIIAQKAQEAEQAAQEARHQATQTLIIKQAIDVCATAAMIADTHHKIVY
                     TNQALEHLMQHMDGALRTEVGSVKAGHLNGIELNRFAHLPNLANTQQQSQTQKNRWHL
                     GHFTLDTAVTPIRGPQGEYLGCVVEWIDRTEELAKHAQETKIAQENARIRQALDASST
                     STLILDEQQLIIYANRAMQTLLTASQRAIASQIPHFNAAAIMGKSSDYVHPHRQLQAA
                     ALKNNLQTVQIDNAIGDKHFVITASPITDNQQQQLGTVIEWRDRSAEIAIEKEINQVI
                     QTASKGDFSALLNLQGKSGFFLNVAQGLNQIITTTKHATDEIIRVIGAMALGDLTQRI
                     SSDYSGEFALVKRDVNKTLDSLTHTMSEIILASETIATSSQQISAGTQDLSTRTE"
BASE COUNT         1182 a         1248 c         1106 g         1097 t
ORIGIN      
        1 cttagaagat gtctatgttt tgcgattttt gacgctcttt gatctaccat taattgccag
       61 tcttctctgg cctgtacttt gagtctttca tcctgcggat cctcaattag tttgtactgg
      121 tagccaacat catctccctt cattacaaac tacctttagg ggtttggggt ttggggttga
      181 gggtccaaaa ccgctccacc gtaatggcta ccacagcaac agaacacagg ataatcggca
      241 ccattaacca gccgcctgcg attaaaatgt tactctccac gacaaactcc cgaagctatt
      301 attgttggcc gggcatgata cccgaatttt tcgcgcggtt aatgaaacag ttcccgcctt
      361 aatcgcctgc agccaggccg ccaagcaagc atagcaccgg tagtcaaaac ccccgctcag
      421 gcggccaaaa cacagctgcg gcacaggctt tgccaggcta atttcacgcc ccccatcaca
      481 cccacccagg ttcccccatg aaactacatt ggcaaattct catcgccatc ggcttggcat
      541 ttatcaccgg cctgctgctg cccaaagaca gccaggtact cggcgtgtcg ctgtacagcc
      601 tgttgggatt tttcggcgcg gcatttttac agggcttaaa aatgctggtg attccgctgg
      661 tgatttcctc ggtgatctgc ggcgttgcag gcattggcag caaccgccta ggccgcatgg
      721 gttggcttac cgccgccacg tttatcggct ttaccgccag cgccgtgttg gtgggtttgt
      781 taatggtgaa cctgtttagc cccggtactc acaacggcaa acccgccagc gatttattaa
      841 acctggtgga cacccagcag gtggccgccc aggtggccaa agtggccgag cgggacggcg
      901 gcgatctagc cggcgtattt ttgcgcatga ttcccagcaa catagtggcc gccgcctccg
      961 atgatggcca aatgctgggg gtcattatgt tttccctatt atttggttat tttttattgc
     1021 agtcggcgga gcctgggcgc agcgcccaat taagtttttg gcgcggcctg aacgacacca
     1081 tgatgcgcat gaccctattc gtgatgaaat tcgcccccat cggtgtatac ggccttatca
     1141 cccaaactgt tatgaatacc ggtttaaaaa gcctttctgt gatgggcgtg ttttttatta
     1201 ccgtggccgc aggtttaata ctgcattgtt ttggcacttt ggcgctgtat ttaaaagtgt
     1261 ttggccgggt caatccgtta aaacactaca aggctatgtt ccccgctatg ctcacggcgt
     1321 tttccaccgc cagctccaat gcgtcactgc cggtgaatat taaatgtttg gaagagaacg
     1381 ccaaggtttc ccacaaaacc acctcgtttg ttttaccgct gggcgcctct ttaaatatgg
     1441 acggcaccgc cttatatgaa tgtgtggcgg tgatttttat tgcccaagcc tacggcgttg
     1501 atttaaccca cagccagcag gtgatggtgg tattaatggc acttttaact tcgtttggta
     1561 ttgcaggtat tcccgctgcc agtttagtgg cgattagcgt aattttaggc agcctgggcc
     1621 tacccataga agctattgcc ttactaatgg tgaccgaccg cattctggat atgatgcgca
     1681 cggcggtaaa tgtttttagc gatagttgct gcgctgtggt ggtggcgcga gtggaagggg
     1741 agaaaggtgt attagtggat tagcactgcg ttcagtgcag cgtctttccc tcgataatgc
     1801 accaacaata ggcacaaata aattgttgaa ttcgccgccg cctaaaaaaa tcttcgtcta
     1861 ggcttaattc accttcaggc gatttattag ggcgacagga tgaaatggtt tcgcgattta
     1921 cccattgtgc gcaaacaaat gttgatgcta atgggagttg cgctgctccc tatccttatt
     1981 ttggggcttt taagttacag caccgcgaaa caagaattaa gtcactccgc caccgaggcg
     2041 ttaattgccg tgcgggattt aaaaaccgcc gcgattaatc gccactttga tcagttgcgc
     2101 ggccaaatta ccagtatggc cctcagcgaa gatgtacaaa tcgcccttgt ggaattaacg
     2161 caaggtttta acagcctaaa ttcagggacc ccacccaata gctcagggct tgccgcctat
     2221 taccaaagcc aattcgccgc tgaatatcaa aaacgcagcc aaaaagccat tgccactcag
     2281 ccgattttag ccgccctctc cccggcgggc ctgtggctgc aaacggaata catcgccaat
     2341 aatgcaaacc ctttaggctc aaaacatctg ctcaacagcg gcgcagatga aactccctac
     2401 aaccgcaccc acgctaaact gcacccgaaa ttgcgccatg aattagaagc ctttgggtat
     2461 tacgatattt ttatcgtcga taacgccagc ggcaatgtgg tgtacaccgt atttaaagaa
     2521 ttggattttg ccaccaattt aaccaccggc agctgggcca aaaccgattt ggcgcgggcc
     2581 tttggccaag ccaaaactct gccgcaaggc aaaactagcc tcacggattt tgccccctat
     2641 ataccatcct atgaagcacc ggcggcgttt ttagccacac cgatttatat ccaaggcaaa
     2701 gccctcggca cgttaattgt gcaggtgcct attgagccga ttaacgccat tatgcaagag
     2761 cgctccggca tgggtaaaac cggcgaaacc tatttagtgg ggccggatta tttaatgcgc
     2821 tcggattcat ttttagaccc cacccaccac agcgtgagtg catctttcgc cgcaccggaa
     2881 accggcagtg ttaaaaccga ggcgattaaa aaagccttaa gcgggcaaac gagcgcagag
     2941 cttttaattg actacaacgg caatccggtt ttatcggcct ttgcgccgct aaaaatagcc
     3001 gatttcagct gggtcattgc cgccgaaatt gaccaagccg aagcgttaaa atccgtgtat
     3061 agcctgcgca ccctgacgct cattattggc gttattttat tagcagcggt ggcggtactg
     3121 gcgctgttta gcgcgcggat ggtcagccag cccattacca gcatggaaca catcatcagc
     3181 cgagtgcaaa aagagggtaa tttccgcctg agtttaaata atcgcgatca ggatgaaatc
     3241 ggcaaaacct gccgcagtgt ggataaacta gtactggata ccgggcgggt aattcaattg
     3301 gtcaatctgc atttgcagca attagtgaga ggggaaacag tcaacgccat aacagaggta
     3361 tttcccggtg atttgggttt attggcccag ggcgttaata gcaccgtcga ggctatagac
     3421 tccgcccgca aagaacaggc ccgccaggcc gtaatcatcg cgcagaaagc ccaggaggct
     3481 gaacaggctg cgcaggaggc gcgccaccaa gctacccaaa ccctcatcat taaacaggcc
     3541 atagacgtgt gcgccaccgc cgccatgatc gccgataccc accataaaat tgtctacacc
     3601 aatcaggctc ttgaacactt aatgcaacac atggatggcg ccttgcgcac agaggtgggc
     3661 agcgtaaaag ccgggcacct aaacggcatt gagctaaacc gcttcgcgca cttgccaaac
     3721 cttgccaata cacagcagca aagccaaaca caaaaaaatc gttggcattt aggccatttc
     3781 acattggata cagccgttac ccccattcgc ggcccccaag gggaatattt aggctgtgtg
     3841 gtggaatgga ttgaccgcac cgaagaatta gccaaacacg cccaggaaac caaaattgcc
     3901 caggaaaatg cccgtattcg ccaggcgctg gatgcctcct ccaccagcac acttattttg
     3961 gatgaacagc agctaattat ttacgccaac cgcgccatgc aaacgctttt aaccgcgtcg
     4021 caaagggcta ttgccagcca gattccgcac tttaatgcgg cggcaattat gggtaaatcc
     4081 agcgattatg tgcacccgca ccggcaatta caggcagcgg cgttaaaaaa taatctgcag
     4141 actgtgcaaa ttgacaacgc cataggcgat aaacattttg tcattaccgc aagccccatt
     4201 accgataacc aacaacagca attaggtacg gtaatcgaat ggcgagaccg cagcgccgaa
     4261 atcgccatcg aaaaagaaat taatcaggtc attcaaaccg cctctaaagg cgactttagc
     4321 gccctgttaa atctgcaggg taaaagcggc tttttcttaa atgtcgccca ggggttaaat
     4381 caaattatca ccaccaccaa acacgccacc gacgaaatta ttcgcgtgat tggcgctatg
     4441 gccctggggg atttaaccca gcgcatcagc agtgattaca gcggtgaatt tgccctggtc
     4501 aaacgcgatg tgaacaaaac cctcgacagc ctcacccaca ccatgagcga aattattctc
     4561 gccagtgaaa ccatcgccac cagcagccaa caaatttccg ctggcaccca ggatttaagt
     4621 acgcgcacgg agg
//
LOCUS       DAOQGS010000328         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9810_length_4629_cov_4.087888, whole genome shotgun sequence.
ACCESSION   DAOQGS010000328 DAOQGS010000000
VERSION     DAOQGS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9810_length_4629_cov_4.087888"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKZ52_12235"
     CDS             <1..838
                     /locus_tag="PKZ52_12235"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNG60584.1"
                     /translation="GDVYLVGGVEHMGHIDMNQAIDPNPLMGLSVAKAAGSMGVTAEY
                     LSLLHGISREQQDAFSLRSHQLAAKARAEGSFGREIVPTPGHNEAGGPVLVTEDETIR
                     ADTTLEALAALKPVFNPNGGSITAGSSSQVTDGASGMLVMSAEKAAALGLTPVAKVVG
                     WSLVGVDPSIMGFGPVPACQKLLQAHGLKMDAIDAIELNEAFAAQALAVLKNLNLLAV
                     MDKKVNLFGGAIALGHPFGCSGTRITGTLISVLAKQGGSRGIATMCIGMGQGIATLIE
                     RL"
     gene            891..1994
                     /locus_tag="PKZ52_12240"
     CDS             891..1994
                     /locus_tag="PKZ52_12240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNG60585.1"
                     /translation="MQKDTRELTALTSLRGVAAVLVVLHHYCLVFTDDIVRWLPSRIL
                     VNAYLCVDLFFMLSGFVLAYVYYSFSEGVTAPDYRRFLLARFARIYPLHLFTLGIVLA
                     LEFAALAVWLNTPSVHSHWAPPFTAEQSLLSLFSNLVMLQTLHWGAFWNVPAWSLSAE
                     FITYLWLPWLIHALAFATIRTCIVVVALSLGCLLAIEAYFGDLGITFAGWPQIVRAGA
                     GAALGGVAWRCYRQSLLPIISGAALLPIFGSTLLALALPLPATALMPVFFCLVLAAAR
                     NQTRPSWLFHNAFSHWLGRISFALYLVHWVVLDTLRQLSLWFTGRGLGANLGLAQELA
                     ALAGLLCLCGLLAWGLQRWVEVPARTWLVRRLN"
     gene            2028..3071
                     /locus_tag="PKZ52_12245"
     CDS             2028..3071
                     /locus_tag="PKZ52_12245"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNG60586.1"
                     /translation="MSQSQIPNCAIAGVASAVPSLRIDNLALEGDFPAAEVRKTVQLA
                     GIRYRYVSDGSICTSDLCLASARQLLDKLKWPVESIDALILVTQSPDYFLPSTASLLH
                     RDLGLATHCAAFDVGLGCSGFPYGLQLAAMLLNFGHKRVLLCQGDTPSLFTSQEDRGT
                     SLLFGDAGTTTALEFSPGAAPWYFNLQSDGRGFDQLIIPAGGFRTRHSDNPRDYCVSM
                     NGAGLFNFTLERVPGLIMDTLAQAGLGVEQVDHYIFHQSNQFMMKHLAKKCGLPEART
                     PIILEDFGNTGGASVPLTLTQALKQQRDDPIKVMLLGYGVGLSWGSALLELPAAAPLL
                     HSELARPLVGVGP"
     gene            3068..3811
                     /locus_tag="PKZ52_12250"
     CDS             3068..3811
                     /locus_tag="PKZ52_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG60587.1"
                     /translation="MNNQEFSGCSILLTGATSGLGAAIAQGLAEAGASLWLTGRNLAQ
                     LEQLAAQLPGQHQCLSLDLSSSSELEAQLNPWLPENLYGFCHCAGAVDTRPLKLMKPA
                     PSALQLQINYAAALEIARIASLRHHMPGAASLLFISSVYANLGAPGQSAYCASKAAIS
                     AAARALAVELAPRSIRVNSLAPGFVATAMTLENAKLSAQALEAIIAKHPLGPGSPEQV
                     ARAALFLLSPQNTWITGTELIIDGGYSAQ"
     gene            3846..4082
                     /locus_tag="PKZ52_12255"
     CDS             3846..4082
                     /locus_tag="PKZ52_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNG60588.1"
                     /translation="MNQAEALALIAEIFECPNNLTPSTQKHNVRGWDSMGVLSLMAEF
                     DDRFGILLTQPQLEQMKTVDDILKILREHGHLAA"
     gene            4085..4624
                     /locus_tag="PKZ52_12260"
     CDS             4085..4624
                     /locus_tag="PKZ52_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60589.1"
                     /translation="MALTLEKITTIIARSLRITPTELYEAFAPATENELADIIQFRQT
                     QLGKSIAWQDEAYLRWRYDFSGNLTSPLNRLWRVKINGELLGLIGLDYVQLNCRGERI
                     DASNPLDLVVLAKLDGLGLGVWMSLVLQQDNGLMFAMGATKSSKTIVQKLFTPMADLG
                     SWKYLLHSSRYLHKKVHPL"
BASE COUNT          911 a         1314 c         1305 g         1099 t
ORIGIN      
        1 aggggatgtg tacctggtgg gcggcgtgga gcatatgggt cacatcgaca tgaaccaagc
       61 catcgacccc aatcctttaa tggggctgtc ggttgccaag gcggcaggct ccatgggggt
      121 tactgctgag tacctatctc tgttacacgg catcagccgg gaacagcagg atgccttctc
      181 cctgcgctcc catcaattgg cggctaaggc ccgcgccgaa ggcagcttcg gccgggagat
      241 tgtgcctacg ccgggccata acgaggccgg tgggccggta ttggtaaccg aagacgaaac
      301 cattcgcgcc gataccaccc ttgaggctct ggccgcactt aaacctgtgt tcaaccccaa
      361 cggtggcagt attactgccg gtagctcctc acaagtaacc gatggggctt ccggtatgtt
      421 ggttatgtcc gctgagaaag ctgctgccct aggcttaacg ccagtggcca aggtggtggg
      481 ctggagcctg gtgggggttg atccgtccat tatgggcttt ggccctgtgc ccgcttgcca
      541 aaaattactg caggctcacg gcctgaagat ggacgccatc gacgccattg agctgaacga
      601 agcctttgcg gcccaggcgc tggcggtgct taaaaacctc aacctgcttg cagtgatgga
      661 caaaaaggtg aacctgtttg gcggcgctat tgcccttggc cacccgtttg gctgctccgg
      721 cacccgtatt accggcaccc tgatcagcgt gttggccaag cagggcggca gccgaggtat
      781 agccaccatg tgtatcggca tgggccaggg tattgccacc ctgattgagc gcctctaacc
      841 cagcaccgca acccatgctg ccacccttca cccgcttgat gtagagatgt atgcaaaaag
      901 atacgcggga gctgacggct ctaaccagtt tgcggggcgt ggcggcggtt ttggtggtgc
      961 tgcaccacta ctgcctggtg tttaccgacg acatagtgcg ctggttgccc tcccgcatcc
     1021 tggtaaacgc ctacctgtgc gtggacctgt tttttatgct cagcggtttt gtgctggcct
     1081 atgtctacta ctcctttagc gagggtgtaa ctgcacctga ttaccgccgg tttttgctgg
     1141 cgcgctttgc ccgcatttat ccgttacacc tatttacgct tggcatagtg ctggcgctgg
     1201 agtttgccgc cttggctgtt tggctgaata ccccgagcgt gcatagccac tgggcgccac
     1261 cctttaccgc cgagcagtcc ctcctgagtt tgtttagcaa cctggtgatg ctgcaaaccc
     1321 tgcactgggg ggcgttttgg aatgtgcccg cctggtcgtt aagtgcggaa tttatcacct
     1381 acctctggct gccctggttg atccatgccc tagccttcgc gactattcgc acctgtattg
     1441 ttgtggtggc cctaagcctt ggttgcctgt tggctataga ggcctatttt ggcgacctag
     1501 ggataacctt tgcgggctgg ccgcagattg tacgggccgg agcaggggct gccttagggg
     1561 gggtggcctg gcggtgttat cggcagagcc tgctgccgat catcagcggc gctgcgctgc
     1621 tgccgatatt tggctcaact cttttggccc ttgccctgcc tctgcccgcc accgcgttga
     1681 tgccggtgtt tttctgtttg gtactggccg ctgcccgcaa tcaaacccgg ccgagctggc
     1741 tatttcataa tgcctttagc cattggctgg ggcggatttc ctttgccctt tacctggtgc
     1801 attgggttgt gttggatacg ctccggcagt tgagcctgtg gtttaccggc aggggcctgg
     1861 gggccaactt ggggctagcc caagagcttg ctgcccttgc gggcctgctc tgcctgtgtg
     1921 gtttacttgc ctgggggctg cagcgctggg tggaggtgcc cgctcgcacc tggttagtcc
     1981 ggcgccttaa ttaataaaaa gtgcatgatg cttaaagagg tatttttatg tcgcaaagtc
     2041 agattcccaa ctgcgccatc gcgggtgtgg cctccgctgt acccagcctg cgcatcgata
     2101 acctcgccct tgagggggat ttcccggcgg cagaggtgcg caaaacagtg caattggcgg
     2161 gtattcgcta ccgctatgtc tccgacggta gcatctgcac cagcgacctt tgtttagcct
     2221 ccgcccggca attgctggat aagcttaaat ggcccgtcga atccatcgat gccctaattt
     2281 tggtgaccca gagcccggat tatttcctgc cctccaccgc cagcctcttg caccgggatt
     2341 taggcctggc cacccactgc gcggcatttg atgtagggct tggttgctcc ggcttcccct
     2401 atggcttgca gcttgcggcc atgctgttga acttcggcca caagcgcgtg ctgctgtgcc
     2461 agggtgatac cccgagcctc tttaccagcc aggaagatcg cggcaccagc ctgttatttg
     2521 gcgatgcagg caccacaacg gccttggaat tcagccccgg cgccgcccct tggtacttta
     2581 acttgcagag tgatggccgg gggtttgacc agttaatcat tcccgctggc ggctttcgca
     2641 cccgtcacag cgataaccct cgggattact gcgtaagcat gaacggcgcg ggcctgttta
     2701 atttcaccct ggagcgggtg ccgggcctga ttatggatac cctggcccag gcgggcctgg
     2761 gggttgagca agtggatcac tacatcttcc accaatccaa ccaatttatg atgaaacacc
     2821 tagccaaaaa atgtggcctg cctgaagcac gcacgcccat tattctggaa gacttcggca
     2881 acaccggcgg cgcttcagta cctttaaccc taacccaagc gctcaagcaa cagcgggatg
     2941 accccatcaa ggttatgttg ctgggctatg gggtagggct ttcttggggt tctgccctgc
     3001 tggaattacc ggccgccgca cccttgctgc attctgagct agcccggcca ctggttgggg
     3061 ttggcccatg aataatcagg aatttagtgg ctgctcaatc ctgttaaccg gcgccacctc
     3121 aggccttggg gcggccatcg cgcaaggcct tgccgaggcc ggggcgagcc tctggctcac
     3181 cggtcgcaac ttggctcagt tggagcaatt ggcggcgcag ttgccggggc agcatcagtg
     3241 cttgagcctg gatttatcca gtagcagcga gcttgaagcg cagctaaacc cctggttgcc
     3301 ggaaaacctg tacggctttt gccactgtgc cggtgcagtg gatacccgcc ccctcaaact
     3361 catgaagccc gccccaagcg ccctacaatt gcaaattaat tatgccgccg ccctagaaat
     3421 tgcccgcata gcgagcctgc gtcaccacat gccaggcgcc gctagcttat tattcatcag
     3481 ttccgtatat gccaacctgg gggcacccgg ccagtccgcc tattgcgcca gtaaagcggc
     3541 gatcagcgcg gctgcccgcg ccctggcggt tgaattagcg ccccgtagca ttcgcgtaaa
     3601 cagcctggcg ccgggttttg tggccacggc aatgacgctg gaaaacgcta agctttcagc
     3661 acaggcgttg gaggcgatta tcgccaaaca ccccttaggc cccggcagcc ctgagcaggt
     3721 ggcgcgggcg gcgctgtttt tgttatcgcc ccagaacacc tggatcaccg gcacagaatt
     3781 aattatcgat ggcggctata gcgcccaata agcctgtagc gcccaataac tttaaggttg
     3841 aagctatgaa ccaagcagaa gcactggcac tgattgcgga aattttcgag tgccccaata
     3901 acctcacacc cagcacacaa aaacacaatg tgcgcggttg ggattccatg ggcgtgttaa
     3961 gcctgatggc agaatttgac gatcgatttg gtattttatt aacccaaccc cagcttgagc
     4021 agatgaaaac cgttgacgat attctcaaaa tcctgcgcga gcacgggcat ttagcggcat
     4081 agttatggcg ctgacgctgg aaaaaataac cacaataatc gcccgcagcc tacgtattac
     4141 tcccaccgag ctgtatgaag ccttcgcgcc agcaactgaa aacgaattgg ccgatattat
     4201 tcaatttcgc caaacccaat tgggtaaaag tatcgcctgg caggacgaag cctatttgcg
     4261 ctggcgctat gatttttccg gcaatttaac ctcgccatta aatcggcttt ggcgggtaaa
     4321 aataaatggc gaactattgg gtttaatcgg ccttgattac gtgcaattaa attgccgcgg
     4381 tgagcgcatt gatgcctcta acccactgga tttagtggtg ctggcgaagc ttgatggcct
     4441 ggggttaggt gtgtggatga gcctggtatt gcagcaggat aacggcctga tgtttgccat
     4501 gggcgccact aaaagttcta aaaccatagt gcaaaaatta tttaccccta tggccgattt
     4561 aggcagctgg aaatatttat tgcacagctc gcgttatttg cataaaaaag ttcatccgct
     4621 gtaattacc
//
LOCUS       DAOQGS010000329         4627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9815_length_4627_cov_4.771216, whole genome shotgun sequence.
ACCESSION   DAOQGS010000329 DAOQGS010000000
VERSION     DAOQGS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_4627_cov_4.771216"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /gene="nirB"
                     /locus_tag="PKZ52_12265"
     CDS             complement(<1..1588)
                     /gene="nirB"
                     /locus_tag="PKZ52_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNG60590.1"
                     /translation="MNKYKILVVGNGMVGHKLIENLVQHPDAQSYEVVTFAEEPRLAY
                     DRVQLSKYFSGATAEDLMLTSEDYYQSNGVRYKLNDKVVAIDAANNCVTTASGHSEPY
                     HALVLATGSYPFVPPLPGKDQPHCLVYRTIEDLEAITASAKVSNVGVVVGGGLLGLEA
                     AKALKDLGLETHVVEFAPRLMAVQLDEAGGSLLRRKIEALNVKVHTEKNTKEIVAGET
                     CRYRMNFADGSFLETDMILFSAGIRPQDELARASGIKIGERGGIVVNNKCQTSVENIY
                     AIGECALWDNKIFGLVAPGYQMAKVAASQLTGGSDEFVGADMSTKLKLLGVDVASIGD
                     AHGRTPGCISYVFQDGVKEVYKRLIVSADGKKLLGAVLVGEVEAYGTLLQICLNQMDL
                     PEDPAALIVPSVDGAAATGMGVDALPESAVICSCHDVSKGTICAAVQAGACTIGDIKS
                     CTKAGTGCGGCVALATQVMNSELTKLGVEVNTDLCEHFAYTRQELYHLVRVGKITTFE
                     ELVEKHGKGLGCDICKPTAAS"
     gene            complement(1603..2412)
                     /gene="cobA"
                     /locus_tag="PKZ52_12270"
     CDS             complement(1603..2412)
                     /gene="cobA"
                     /locus_tag="PKZ52_12270"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNG60591.1"
                     /translation="MPPVIPSFSSSSQASGKVWLVGAGPGDPELLTLRALRVIEQADL
                     ILFDQLVSPEIRALFPKQVPQFYVGKSKGQHSIPQHNLNALLVKKARQGLQVVRVKGG
                     DPFVFGRGGEELLVLRKAGIEAEVVPGITAASGASTYAGIPLTHRGLAQGCTLITAQG
                     ETDTAINWAALVGLNHTLVFYMGLSRAQWIADELQAAGMGANKPVAVIENGCRSNQRV
                     VITELADLAASVASHALQSPSLIVVGEVVRLAQQLQTPAHDFVTTCQRLSA"
     gene            2936..3553
                     /gene="gmk"
                     /locus_tag="PKZ52_12275"
     CDS             2936..3553
                     /gene="gmk"
                     /locus_tag="PKZ52_12275"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNG60592.1"
                     /translation="MAIGGTLFTVSAPSGAGKTSLVNALVAATPGVQVSISHTTRAMR
                     PGEVNGVNYHFVEPPQFLAMLGEGAFLEHAQVFNHYYGTSSTWVNETLASGVDVILEI
                     DWQGAAQVRRQIPEAVSLFILPPSQACLLERLTGRGQDAQAVIDARMAEAKSEISHYV
                     ESHYIIVNDDFNQALKEFQAIVIAQRLSQSKQSERHAELLSSLLA"
     gene            3750..4013
                     /gene="rpoZ"
                     /locus_tag="PKZ52_12280"
     CDS             3750..4013
                     /gene="rpoZ"
                     /locus_tag="PKZ52_12280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNG60593.1"
                     /translation="MARITVEDCLDHVANRFELVIVGSKRARQLAIMGKQPLVPAEND
                     KPTVLALREIEKGLISVDILKEPDEQRVVVPKHIPPVDSSFDE"
     gene            4032..>4627
                     /locus_tag="PKZ52_12285"
     CDS             4032..>4627
                     /locus_tag="PKZ52_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNG60594.1"
                     /translation="MLTIDSLCHRLSSYLEPAQINRVRRAYYYAEQAHTGQFRRSGEP
                     YITHPLAVANILAGMHMDHQSLMAAMLHDVIEDTGIGKQALHEQFGEVVAELVDGVSK
                     LAKIEYESQAEKQAKNFQKMALAMARDLRVIVVKMADRLHNMRTLGALAPEKKRRIAK
                     ETLEIYASIAHRLGMNDLRIEFEDRCFTAMFPLRAARL"
BASE COUNT         1132 a         1298 c         1125 g         1072 t
ORIGIN      
        1 tagacgccgc cgtgggttta caaatatcac agcccaggcc tttgccgtgt ttttctacta
       61 attcttcaaa ggtagtgatt ttacctaccc gcaccaagtg atacagttcc tggcgggtat
      121 aggcaaagtg ttcgcataaa tcggtattta cctctacgcc taatttagtt aattcgctgt
      181 tcatcacttg agtggccagc gccacacaac cgccgcagcc ggtgcccgct ttagtacagc
      241 ttttaatatc accaatggtg caggcacctg cttgcacggc agcgcaaata gtgcctttgc
      301 tcacatcgtg gcaggagcaa ataaccgcgc tttcaggcag cgcatccacg cccattccag
      361 ttgcggcggc gccatccaca gacggcacaa ttaaggccgc ggggtcttcc ggtaaatcca
      421 tttgattaag gcaaatttgc aataaggtgc cataggcttc cacctcgccc accagtacgg
      481 cgccgagtaa ttttttgcca tcggccgaaa caattaagcg tttgtatact tcttttacgc
      541 catcttgaaa cacgtaagaa atacagcctg gcgtgcgacc gtgggcatcg ccgatgctgg
      601 ctacatccac acccaacaat tttaatttgg tgctcatatc ggcacccaca aattcatcgc
      661 tgccgccggt taattggctg gcagcgactt ttgccatttg ataaccgggc gccaccaaac
      721 caaatatttt gttatcccac agggcgcact cgccgatggc ataaatattc tccaccgagg
      781 tttggcactt gttgttcacc acaatgccgc cgcgctcacc aattttaata ccgcttgcgc
      841 gcgctaattc atcctgcggg cgaataccgg cggagaataa aatcatgtcg gtttctaaaa
      901 aggagccgtc ggcaaaattc atgcggtagc ggcaggtttc cccggcgaca atttctttgg
      961 tatttttttc ggtatgcact tttacattca gcgcttcgat tttccggcgc agcaaactgc
     1021 cgcctgcctc atccaattgc acggccatca agcgcggcgc aaattccacc acatgggttt
     1081 caaggcccaa atcttttagc gccttagccg cttccaaacc caacaggcca ccgcccacta
     1141 ccacgcccac gttgctcact ttggccgagg cggtaatggc ttctaagtct tcaatggtgc
     1201 ggtacaccaa acaatggggc tgatctttac cgggcagcgg cggcacaaac gggtaagagc
     1261 ctgtggccaa taccagcgcg tggtagggct cgctgtggcc actggcggtg gtcacgcagt
     1321 tattggccgc atcgatggcg actaccttgt catttaattt ataacgcaca ccgttggatt
     1381 gataataatc ctccgaggtg agcattaaat cctccgccgt agcgccggag aaatatttgg
     1441 acaattgcac tcggtcgtaa gctaggcgcg gctcttcggc gaaggtaacc acttcatagg
     1501 attgggcgtc cgggtgttgg actaaatttt caattaattt gtggccgacc atgccgttgc
     1561 cgaccaccag tattttgtat ttgttcatga taaagcctct ggttaagccg acagacgttg
     1621 gcaggtggtt acaaaatcgt gcgctggggt ttgcagttgt tgcgccaagc gcaccacctc
     1681 acccacaaca attaaagaag gcgattgcag ggcgtggctg gcaacgctgg ccgccaaatc
     1741 ggcaagctcg gttatcacca cccgctggtt tgatcggcag ccattttcaa tcacggccac
     1801 aggtttattc gcccccatgc cggcagcctg cagttcatcg gcaatccact gggcgcggct
     1861 caggcccatg taaaacacta gggtgtggtt aagacctacc agcgccgccc aattgatggc
     1921 ggtgtcggtc tcaccctgtg cggtaatcag cgtgcagccc tgggccaggc cgcgatgggt
     1981 taaaggaata cccgcgtagg tgctagcacc ggaggcggcg gtgatgccgg gcaccacttc
     2041 cgcctcaata ccggccttgc gcaacaccaa caactcctcg ccgccgcggc caaaaacaaa
     2101 cgggtcgccg cctttaacac gcaccacctg taagccctgg cgcgcttttt tgaccagtag
     2161 ggcgttgagg ttgtgctgag gaatactgtg ttgccctttg cttttaccta cgtagaactg
     2221 gggcacctgt ttgggaaaga gggcgcgaat ctctgggctt accaattggt caaataaaat
     2281 taaatcggcc tgctcaatca cgcgcagggc gcgcagggtt aataactcag gatcaccggg
     2341 gcctgccccc accaaccaga ctttgccgga tgcttgggag gacgagctga aagaaggaat
     2401 tacgggcggc ataaaaaata cttcgcggct taggaaacag attaaaagca agcccacctg
     2461 ggtaaggcgg ccaaacaccc ggaaacaaaa accctgattc gagccaactt gctgcctatt
     2521 cgctaaagca caatatgagc caacccctat aatttattat aagtctttat aattcaatta
     2581 gttataaata tcacatgggc tcaacaggcc gttcaattcc tcacaaacgc accaataaaa
     2641 atcattaagc cctattggtg agcttttaat catcttcgac aggtatcgtt tgtgtttttt
     2701 agtggtgcct attcgcacca tatagataca ttaattttca ctaaaaaatt tcaaccagag
     2761 cgatgcctaa gttttttgca ttatttttgt gcatatgagc ggtaaaggcc tgagatgctg
     2821 tccccttgcc gaatcagcta tcattacccc atacacattc ccagcgcatg tgcgctttcg
     2881 accgcgcatt gcagaacccc acggcaccgg cacaccacca acaagagcaa ttagcatggc
     2941 aattggcggc actctattta cagtttcagc tccgtctggc gcaggaaaaa ccagcctagt
     3001 aaatgcccta gtggccgcca caccaggcgt tcaggtatct atttcccaca ccacccgcgc
     3061 catgcggccc ggtgaagtca acggcgtcaa ctaccacttc gtggagccgc cacaattctt
     3121 agcgatgctg ggcgaagggg cctttctgga gcatgctcaa gtgtttaatc actattacgg
     3181 cacctcctcc acctgggtga atgagaccct ggcctctggc gtggatgtga ttttagaaat
     3241 tgactggcag ggcgcagccc aggtgcgcag gcagataccg gaggcggtca gcctatttat
     3301 tctcccccct tcccaggcct gcctgctgga gcgcttaacc ggccgcggcc aagacgccca
     3361 agcggtgatc gatgcacgca tggccgaggc gaagagtgaa atttcccact atgtagaaag
     3421 ccactacata atcgtcaatg acgatttcaa ccaagctctc aaagaattcc aagccatagt
     3481 cattgcccag cggttgagcc aaagtaaaca atctgagcgc cacgccgagc ttttatcgag
     3541 cctgttggcc tgacccaggc tgtggctgtg gcgcaaaaag gttgagcggc atgatcgatt
     3601 tgcgtacact tcgtaaccct attttttaat gggtcagcca gctcaacacc ctaagcccat
     3661 ctggcctgta acgcccagcg taaacaggct taggctgacc ccagacgggc acaccccaaa
     3721 cttccttcat tttattaggc accttattca tggcacgtat cactgttgaa gattgtttag
     3781 accacgtagc aaaccgcttt gagttggtaa ttgtgggcag taaacgcgct cgccaattgg
     3841 ccattatggg caaacaaccc ttggttcccg ccgaaaacga caaacccacg gttttagcat
     3901 tgcgggagat tgaaaaaggc ctcatcagcg tcgatattct gaaagaaccc gatgaacagc
     3961 gtgtggtagt gcccaagcac atcccacctg tcgactcatc cttcgacgag taaatctatt
     4021 aggagggcgt cttgttaacc attgactcgc tttgccacag gctgtcctct tatctagaac
     4081 ccgcccaaat caaccgtgtg cgccgcgcct attattatgc ggagcaggcg cacaccggcc
     4141 agttccgccg cagcggcgaa ccctatatca ctcaccccct agcagttgcc aatatcctcg
     4201 ccggtatgca catggaccat caaagcctga tggcagccat gctgcacgat gtcattgaag
     4261 ataccggcat aggcaaacag gccctgcacg agcaatttgg cgaggtggtg gcagaactcg
     4321 tggatggggt gagtaaactt gccaaaatcg aatacgaatc ccaagcggaa aaacaggcca
     4381 agaactttca aaaaatggcc ctggccatgg cgcgggacct gcgggtgatt gtggttaaaa
     4441 tggccgaccg gctgcacaac atgcgcactt taggtgccct agcgccggaa aaaaaacgtc
     4501 gcatcgccaa agaaacctta gaaatttacg cctccattgc ccaccgtttg ggcatgaacg
     4561 atcttagaat cgaatttgaa gatcgctgtt ttactgccat gtttcccctg cgggcagcac
     4621 gtttaca
//
LOCUS       DAOQGS010000330         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9859_length_4616_cov_4.166411, whole genome shotgun sequence.
ACCESSION   DAOQGS010000330 DAOQGS010000000
VERSION     DAOQGS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9859_length_4616_cov_4.166411"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKZ52_12290"
     CDS             <1..545
                     /locus_tag="PKZ52_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60595.1"
                     /translation="SAFATLACFQQSPRFVQQADQYWQINTVPSGPLAGKPALLKQAE
                     PFAGRINGSVQRQECSGAEAKPLIKTSKASVKGTLSVEGDAVKLILSVTDLKDKIVKE
                     ERLEFLGHSPTVNFTAGAAGEFALIQKLRANAKPGDKISLLRESEYSLTFTKKFLTIR
                     AVHFANGLAYAEDVWEITPR"
     gene            665..1450
                     /locus_tag="PKZ52_12295"
     CDS             665..1450
                     /locus_tag="PKZ52_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNG60596.1"
                     /translation="MRVLLRFLGAFVVILAQPGWAISNVEADKPRLPNEGFSGALGLA
                     AEGKKGNQEKQAFSGASRLNYRQEDTLILLMLAQDYGSKNKVRDDRSQFAHLRWTNLL
                     SEKLATEVFAQWETEEFDYLKSRRLAGAGLRYFVGYKPDVYLCALGLGAFKEKEVNDL
                     STYIDEQNMWRMNAYYSLKVQINNQLSLVNTIYAQPNLDDFSDYRILFDLTLSSKVTE
                     HLAFSLAYRLRHDSQPAQNLAATPPIINHASNMEYKSGLTYSF"
     gene            1487..2437
                     /locus_tag="PKZ52_12300"
     CDS             1487..2437
                     /locus_tag="PKZ52_12300"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNG60597.1"
                     /translation="MKLLNATHNPLGLGPTLLVCALSIGLPLFWDIPGHWRLIGALQN
                     SGHAVLFFFMAFCLAWRKLPGQWLLLALCALGLSIEAVQYFIGKDCDIQDVLLDNLGS
                     ISGWLSFKLIKNRSLGRAVFPLGMLPVCLMVAAFYKPCLIALCYAMQWQNFPTLVSFD
                     ELGRGFLIDHHEGGEYALTRLPAEIIQQGGPVNRTRVLQLNCPAQNWPGVALIDLPPN
                     WQGFTQVQLDVWLADKTPIHLGLALRGLNNQSDHHDVSQRFLLQPGYNRVRWRLEDVR
                     SKASNQNLLAEIDKIIIFCMPEETRKQVSSLWVDDLRLVE"
     gene            2754..3659
                     /locus_tag="PKZ52_12305"
     CDS             2754..3659
                     /locus_tag="PKZ52_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNG60598.1"
                     /translation="MSIPTIIKQINTIVLGKDTQVRLSLACLLAQGHLLIEDLPGMGK
                     TTLAHCLAKVLGLKFERVQFTADLLPADILGVAVFAREQGVFRFHPGPIFTQVLLADE
                     INRASTKTQSALLEAMEEGQVTVEGKTRPLPTPFFVIATQNPLSQSGTYHLPESQLDR
                     FLMRISIGYPTAQSERMLFEGINPRQALEALQPVINREALLDLQSQVRSVTASHALLD
                     YLQRLVAATRQEAIFSYGLSPRGALAWLNAAKAWAFIHERNYLIPEDLQAVMQAVLGH
                     RLKGARQQDVAQLLKELQQRVPVLA"
     gene            3662..4615
                     /locus_tag="PKZ52_12310"
     CDS             3662..4615
                     /locus_tag="PKZ52_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNG60599.1"
                     /translation="MLSWWDNYWHQWLLRRQPKASVFTLNRRNLYTFPGTSGFVYLGL
                     TLVLWLIGTNYQNNLILALAYGLISVFVIAILHAYANLAGIKLEALPSHAVFAEETAQ
                     VKLRLTATNKRGGENIRCLFRGQTGVAVELPCQQPCVINLPVSTQKRGWLQPGRLLIE
                     TYYPLGLIRCWTWLVFDVELLVYPKPLRKELPSLSKSGSGEQIGAIKLGGSDFEGIKN
                     YTPGDSRKHIAWKAVAKGKGLLTKYYVEDIAQEQWLDLNDLPNSLEERLSILTYWVLQ
                     LSEQNQVFGLRLNAQTISPNQGSAHRLACLSALVGGYGLGI"
BASE COUNT         1118 a         1121 c         1157 g         1220 t
ORIGIN      
        1 gctcggcatt tgccaccctg gcctgctttc agcaaagccc tcgttttgtt cagcaggcgg
       61 atcaatattg gcaaattaat actgttccca gcgggccgct agcgggtaaa ccagccttgt
      121 taaaacaagc agaaccgttt gcaggccgca tcaacggctc tgtgcagcgg caggaatgca
      181 gcggtgcaga agccaaaccc ctaattaaaa ccagtaaagc tagtgtaaaa ggtactttaa
      241 gtgttgaagg ggatgccgtt aaattaattt tatccgtaac agaccttaaa gataaaattg
      301 ttaaagagga gcgcctggag tttttaggcc actcacctac agtgaatttt accgcaggcg
      361 ccgctggcga attcgcgctt atacaaaaac tgcgcgccaa cgcgaaacct ggcgataaaa
      421 tctctctgct gcgggagtcc gaatattccc tcaccttcac taaaaaattc ctcaccatcc
      481 gcgctgtgca ttttgccaat ggtctagcct atgcagagga tgtatgggag attacgcctc
      541 gctgatgccg ttttacccgc gggatagtta aacccctgtc agcatttaat ttatgtattc
      601 aatccgggcg catccagcgc ctttattttt tctattgttt taatttttat caataaggtt
      661 agttatgcgt gtgttgttac ggtttttagg tgcttttgtg gtgatattgg ctcagcccgg
      721 ctgggctatc tccaatgtgg aggcagataa accgcgcctg cccaatgagg gcttttccgg
      781 tgcgctgggc cttgcggcgg agggcaaaaa aggcaaccag gaaaaacaag ccttttcagg
      841 cgcaagccgc ctaaattacc ggcaggagga cacgctaata ttattaatgc tggcccagga
      901 ctatggctca aaaaataagg tgcgggatga ccgcagccag tttgcccacc tgcgttggac
      961 caatttattg agcgaaaaac tcgccactga agtgtttgcc cagtgggaaa cagaagaatt
     1021 tgattattta aagtcccgcc gcttggcagg tgctggcctg cggtattttg tgggctacaa
     1081 accggatgtt tatttgtgcg cgctggggct gggtgcgttt aaagagaaag aggtaaacga
     1141 tttaagtacg tatatagatg agcaaaatat gtggcgcatg aacgcctatt actcgctcaa
     1201 agtgcaaatt aataatcaat tgtccttggt caataccatt tacgcccagc ctaatttaga
     1261 cgattttagt gattaccgga ttttgtttga tctaacactc agcagcaaag tgactgaaca
     1321 cctggcattt agcctcgcgt accgattgcg ccacgacagc cagcctgcac aaaacctggc
     1381 ggcaacgcca ccaattatta atcacgccag taatatggaa tataaatctg ggctcaccta
     1441 ttcgttttag ctcagccaca gccacagcta tgcgcgcttc tgtgcaatga aactgcttaa
     1501 cgccacccac aatccactcg gtttagggcc aactctgctg gtctgtgcct taagtatcgg
     1561 cctgccttta ttttgggata tccccggcca ttggcgcctg atcggcgcgc tgcaaaattc
     1621 cgggcatgcg gtgttatttt ttttcatggc cttttgtttg gcctggcgaa aattgcctgg
     1681 ccagtggttg ttattggctt tgtgtgccct gggcctgagt attgaggccg tgcaatattt
     1741 tatcggcaaa gattgtgata ttcaggatgt gctgctggat aatttgggtt caattagcgg
     1801 ctggttgtcg tttaagctca ttaaaaaccg ctcgctgggc cgcgcggttt tccccttagg
     1861 tatgttgcct gtatgtttaa tggttgcagc cttttacaag ccgtgtctaa ttgcactgtg
     1921 ttacgcgatg caatggcaga attttccgac gctggttagc tttgatgagc tcgggcgcgg
     1981 ttttttaatt gatcaccatg aaggcggtga atatgccctc actcgtctgc ccgcagaaat
     2041 tattcagcag ggtgggccag ttaaccgcac gcgggtatta caactgaatt gcccggcgca
     2101 aaattggccg ggtgtagcct taattgattt gccgcccaat tggcaggggt ttacccaggt
     2161 gcaattggac gtatggctgg cggataaaac gccgattcat ctagggctcg ccctgcgcgg
     2221 ccttaataac cagtccgatc atcacgatgt gagccagcgc ttccttctgc aacccggcta
     2281 caaccgggtc cgctggcgcc tggaggatgt tcgcagcaaa gccagcaatc aaaatttgct
     2341 agccgaaata gacaaaataa tcatattttg tatgcctgag gagacacgca agcaagtgtc
     2401 atccctgtgg gtggacgacc tgcgcctggt agagtgacgg cttttagcgt ctacaaaggc
     2461 ttgagggcac ctgcggatat tcaattttcg tgatgctcgc taggcatttt cgagcggaag
     2521 caggcagttt atgcagtata aatgaccgac tgagcacgaa aataacgacg tgagggcacg
     2581 aaaagtggat ttccgcaggt acccttaagt ttctgcccgg aagactgggg gctcttgcta
     2641 cacttaacag catgttgcgt tgtttaaacc tagtcattgg cctataaagc ctaagccgcg
     2701 cgctaaacca tttaggctct gatcgttatt tacctcccag gatttaccca ggaatgtcca
     2761 tccccactat tattaagcaa ataaatacca tcgtgttggg taaagatacc caagtgcgtt
     2821 taagccttgc ctgtcttttg gcgcaggggc atttattaat tgaagatttg cccggtatgg
     2881 gcaaaacaac cctggcccat tgtttggcga aggtgttggg gcttaaattt gagcgggtgc
     2941 aatttaccgc cgatttatta ccggccgata ttttaggcgt ggcagtattt gcgcgggagc
     3001 aaggggtttt tcgttttcac cccggcccta tttttaccca agtattattg gccgatgaaa
     3061 taaaccgcgc ctccaccaaa acccaaagcg cgctgcttga agccatggag gaagggcagg
     3121 tgacagtaga aggcaaaacc cgcccgctgc caacaccttt ttttgtaatc gccacgcaaa
     3181 accccttaag ccaaagcggc acttatcacc tgccggaatc ccagttggat cgctttttaa
     3241 tgcgtatttc catcggttat cccaccgcgc agtccgagcg aatgttattt gaaggcatta
     3301 atccgcgcca agccctagag gcgctgcagc cggttattaa ccgagaagcc ctgcttgatt
     3361 tacaaagcca agtgcgcagt gtcaccgcaa gccacgcatt attggattat ttacagcgcc
     3421 ttgttgccgc cacccggcaa gaggcaattt ttagttatgg gctatcaccg cgcggcgcct
     3481 tagcttggct taatgccgct aaagcctggg cgtttattca cgagcgcaat tatttaattc
     3541 ctgaagattt gcaggccgtt atgcaagctg ttttaggcca ccgtctaaaa ggcgcgcgcc
     3601 aacaggatgt ggcgcaatta ttaaaggaat tacaacagcg cgtacctgtg ttggcttaag
     3661 tatgctcagt tggtgggata attattggca ccagtggcta ttgcggcgcc agcccaaagc
     3721 ctctgtgttt acgctcaatc gtcgcaattt atacaccttt cctggcacct cagggtttgt
     3781 atatttgggg cttactttag tgctgtggtt aattggcacc aattatcaaa acaatttaat
     3841 ccttgccctg gcctatgggt taatcagtgt atttgttatc gccattttgc acgcctacgc
     3901 caatttagcg ggcataaaat tggaggcctt accctcgcac gctgtttttg ccgaggaaac
     3961 tgctcaggtt aagctgcgcc ttacggccac caataaacgc ggtggcgaga atattcgctg
     4021 tttatttcgc ggccaaacag gtgttgccgt tgagctgccc tgccaacagc cctgtgtaat
     4081 taacctgccc gttagcacac aaaaacgcgg ctggttgcaa ccgggccgct tattaattga
     4141 aacctattat cccctggggt taatccgctg ctggacttgg ctggtatttg atgtggaatt
     4201 attggtttat ccaaaaccgc tgcgcaaaga attacctagc ctttcgaaat ctggcagcgg
     4261 cgagcagata ggtgcaatta aattaggcgg tagcgatttt gaagggatta aaaattacac
     4321 ccccggcgac tcgcgcaaac acatcgcctg gaaggccgtt gccaaaggca aaggcttgtt
     4381 aaccaaatat tatgtagagg acattgccca ggagcagtgg ctggatctaa acgatctgcc
     4441 caactccctg gaggagcggc tgagtattct cacctactgg gtactgcaac tgagcgaaca
     4501 aaaccaggta tttggcttgc gcttaaatgc ccaaaccata tcccccaacc aaggcagcgc
     4561 ccaccggctg gcctgtttaa gcgcgttggt tgggggatat ggtttgggca tttaag
//
LOCUS       DAOQGS010000331         4612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9872_length_4612_cov_4.842879, whole genome shotgun sequence.
ACCESSION   DAOQGS010000331 DAOQGS010000000
VERSION     DAOQGS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9872_length_4612_cov_4.842879"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKZ52_12315"
     CDS             complement(<1..348)
                     /locus_tag="PKZ52_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNG60600.1"
                     /translation="MRTFLTSPFFTWIRRLQFPLAIVIFTYYALLPGTQVHLVLSDKT
                     THFLGNATLYLSAWVALGGRFRLLLLISLLIPYSLFIELCQYFSPGRTVDVRDMVARE
                     ASLPVRDLRRSIRR"
     gene            complement(360..1739)
                     /locus_tag="PKZ52_12320"
     CDS             complement(360..1739)
                     /locus_tag="PKZ52_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60601.1"
                     /translation="MRFSSSSLSLSVRLFLASAVLLPPLLAFSSHMLNHAMLRSQQTA
                     ERNLLRGQIHLLLGAAQPNKKGLKLPDNLPESRFSTVNSGLIGWILDSNDKIYWMSKS
                     GDLVEKTELPQFNQPFVAGKEYYASFTLGGEEFYALTYDTLWEINKQDKNYRFVVAHN
                     QGPMQAELNAYQKRLWKWLAGLALLLVIVQTLVTRWGIQPLRRLARDLEQVEAGLSQE
                     LTGRYPADIQPVTNNLNRVLRNELAQKERYRNSLNDLAHSLKTPLSVLRGQLQNRDKE
                     QLMQVVDEQVNRMTAIVDHQLRRATAQVTQTLAHNMLDLTPLVQRLINAMHKVYQNKG
                     IQCTSTLPPQLKFPGDEADMMELIGNLIENACKYGQTKVQISAKIEERPIGKELVIVV
                     EDDGPGIPTSLHDTILTRGARADTATAGQGIGLAVAVDILSSYGGSLEITTAALGGAG
                     FILRLPYKT"
     gene            complement(1797..2489)
                     /locus_tag="PKZ52_12325"
     CDS             complement(1797..2489)
                     /locus_tag="PKZ52_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG60602.1"
                     /translation="MRLLIVEDEQAIRLQLQEFLMEQGFAVDTAADGREGLYFATEYD
                     YDLAIIDIGLPHLDGINLIKKARAAGKLYPILILTARSNWQDKVEGLESGADDYLVKP
                     FHNQELRARLNALIRRASGHASPVLEFGPLALDTAAKSLSVNGAEVELTSYEYNTLEY
                     LALHAGQVISKTQLTEHLYQQDFERDSNVIEVFIGRLRKKIDLGPDQEAITTLRGRGY
                     RFNLPRTNKASA"
     gene            complement(2520..2795)
                     /locus_tag="PKZ52_12330"
     CDS             complement(2520..2795)
                     /locus_tag="PKZ52_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60603.1"
                     /translation="MLISLACIQASAEESSISRTQAAQMAQQKFGGKVISIDEVEPAP
                     AASDEDASAQPAPPSQNNPRFVVKLMQSGRVQVVNLDAQGQPLNPAP"
     gene            complement(2823..3368)
                     /locus_tag="PKZ52_12335"
     CDS             complement(2823..3368)
                     /locus_tag="PKZ52_12335"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNG60604.1"
                     /translation="MLSSRAFPRWIFCALVGSAGLTHQAVAAGYDQTEDFARVVSSRP
                     IYRSVEERIPSQRCWTETVREEPRNDNSPAGAIVGGVVGGALGHAVGHGSGNKKIGTA
                     VGAVLGATVGNEVAKNRAQADEGGYRERERCQETSRTERREEIIGYDVDYQYQGRTYS
                     TRMDHQPGSRIRVAVRVEPLE"
     gene            3478..4149
                     /locus_tag="PKZ52_12340"
     CDS             3478..4149
                     /locus_tag="PKZ52_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60605.1"
                     /translation="MQILLIEDDKSLATGMQEALKNAGLVVNWVDRGQEALQSLAVQV
                     PDIVILDLGLPDMDGLSLLKTLRRQHKQVQVLILTARDSVVDKVAGLDVGADDYLTKP
                     FDLDELLARLRAFERRLGTASSHLIVHGRITLNLRDRIALVDDAELSLSKREFMLLKA
                     LMEQQNRVHTKESLEAKLYSWGEEVVSNTIEVHIHHLRKKLPENSIQTLRGVGYRLTK
                     IDTAG"
     gene            4153..>4612
                     /locus_tag="PKZ52_12345"
     CDS             4153..>4612
                     /locus_tag="PKZ52_12345"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNG60606.1"
                     /translation="MGSIRLFLILLLISVVTIANFTAAVRGYVGSMQEAEAFFDERLE
                     AQLEYIHASLGLALRQPGVQTPLKIVPSLSLHRPLPLNYHIEFQVFHPVNGLVARSED
                     FPVQALVVWQEGFSEFEYEGVRWRGRIHWDEPLGLWYLVAERFDQRYSLAE"
BASE COUNT          965 a         1197 c         1381 g         1069 t
ORIGIN      
        1 ccggcggatg ctgcgtcgca gatcccgcac cggcaggctg gcctcccggg ccaccatatc
       61 gcgcacgtcc acagtgcgac cgggggaaaa gtactggcac agttcgataa acagggagta
      121 ggggatcagc aggctaatca acagcagcag acgaaagcgc ccgcctaagg ctacccaggc
      181 tgataaatat agggtggcat tgccaagaaa atgggtggtt ttatccgaga gcaccaggtg
      241 cacttgagtg ccgggcagca gggcgtaata ggtgaagata acaatggcca gggggaattg
      301 cagccgacga atccacgtga agaaggggga cgttaaaaaa gttcgcataa caaaattaat
      361 caggttttat agggtagacg cagaataaat ccggcgccgc caagagctgc ggtggtaatt
      421 tccaggctgc caccatagct gctgagaata tccaccgcaa cggcaaggcc tatgccttgc
      481 ccggctgtgg cggtatcggc gcgggcgcct cgggtcagga tggtgtcatg caggctggtc
      541 ggaattcccg gcccatcatc ctcaaccact atgaccaatt ctttaccgat gggccgttcc
      601 tcaattttag cggagatttg caccttggtt tgcccgtact tacaggcgtt ttcgatcaga
      661 ttgccaatca gctccatcat atccgcctcg tcgccaggga atttaagctg gggcggtagg
      721 gtgctagtgc attggatccc cttattttga tacaccttgt gcatggcgtt gatcaggcgc
      781 tgcaccaggg gtgtaaggtc gagcatattg tgggcgaggg tttgcgtcac ctgagcggtg
      841 gcgcggcgca gttggtgatc cactatggcc gtcatgcggt tcacctgctc atccaccacc
      901 tgcatcagct gctctttatc gcggttttgc agttgccccc gcagtaccga aagcggtgtt
      961 tttaggctgt gggcgagatc gtttagggaa ttgcggtagc gctctttctg ggccaattcg
     1021 ttgcgcagta cccggttgag gttattggtg accggttgaa tatccgcagg ataccggccg
     1081 gttagctcct ggctcaggcc cgcctccacc tgttctaaat cccgcgccag ccgccgcagg
     1141 ggttgaatac cccagcgggt tacgagagtt tgtacgatta ctaggagcag cgctaagccc
     1201 gccagccact tccagaggcg tttttgatag gcattgagtt ccgcctgcat ggggccttgg
     1261 ttgtgggcta ctacaaagcg gtagttttta tcctgtttgt taatttccca cagggtgtcg
     1321 taggtgaggg cgtaaaactc ctcgccgccg agggtaaagc tggcgtagta ctccttgccc
     1381 gccacaaagg gttggttaaa ttgtggcaac tcggtttttt ccaccaaatc gccggatttg
     1441 gacatccaat aaatcttgtc attgctgtcg agtatccagc cgataagccc tgaattgacg
     1501 gtactaaagc gggattccgg caggttatcc ggcagtttga ggcccttttt attgggttga
     1561 gcggcgccta acaagaggtg aatttgcccg cgcaataggt tgcgctccgc cgtttgctgg
     1621 ctgcgcagca tggcgtggtt gagcatgtgg gaactaaacg ccagcagtgg cggcagcaat
     1681 accgccgagg cgagaaataa gcgaacgctt agggataatg acgagctgga aaaacgcacg
     1741 ttcaggtctc aagccccaac gggtgccaaa caaacccggc aagggtgggc atagccctag
     1801 gcactggctt tgttggtgcg cggcaggtta aacctatagc cgcgcccgcg cagggtggta
     1861 atggcctctt gatctggccc caggtcaatc ttcttgcgca ggcggcctat aaacacctca
     1921 atcacgttgc tgtcccgctc aaaatcttgc tggtacaggt gttcagttag ctgggttttg
     1981 gagatcacct ggcctgcgtg cagggccaga tattcaaggg tgttgtattc atagctggtc
     2041 aactccactt ccgcgccgtt cacacttaag ctttttgcgg cagtatcaag ggctagaggg
     2101 ccgaattcca agactgggct ggcgtggccg gaggcgcggc gaattaaggc gtttaagcgg
     2161 gcgcgcagct cttggttgtg aaagggtttc accaggtaat catcggcgcc ggattccaac
     2221 ccctcaactt tgtcctgcca gttgctgcgg gcggtgagaa tcaaaatagg gtagagcttt
     2281 cccgctgcac gagctttttt gatgaggtta atgccgtcta aatggggtag gcctatgtcg
     2341 ataatggcga ggtcgtagtc gtattcggtg gcgaagtaca agccttcacg gccatcggcg
     2401 gcggtgtcca ccgcaaaacc ctgctccatt aaaaattcct gcaattgcaa gcggatggcc
     2461 tgttcatctt caactatcaa aagtcgcatg gggtaccttg tggggagtgt gcgttgatat
     2521 tagggcgcgg ggttgagcgg ttggccctgg gcgtcgagat taaccacctg cacgcgcccg
     2581 ctttgcatga gtttaaccac aaagcgcggg ttgttctgac tgggcggcgc gggctgggcc
     2641 gaggcatctt cgtcacttgc cgccggtgca ggctccactt catctatgct gatgaccttg
     2701 ccgccaaatt tctgctgcgc catttgtgca gcctgggtgc ggctgatgga agactcttca
     2761 gcactggctt ggatacaggc caaactgatg agcaagcagt aacaccagcg catcacaaac
     2821 ggttactcga ggggctcgac gcggacggca acgcggatac ggctgccggg ttggtgatcc
     2881 atacgggtgc tgtaggtgcg gccctgatat tggtagtcca catcgtagcc gatgatttct
     2941 tcgcgccgct cagtgcggct ggtctcctgg cagcgctccc gctcccggta gccgccctcg
     3001 tcagcctggg cgcgattttt cgccacttca ttacctacgg ttgcacctaa aacggcgcct
     3061 actgccgtgc cgattttttt attaccgctg ccgtggccca ctgcgtgtcc gagggcaccg
     3121 cccactacgc cacccactat ggcacccgct ggtgagttgt cgttacgggg ttcttcgcgc
     3181 acagtctcgg tccaacagcg ctgggaagga atacgctcct ccacgctgcg gtagatgggc
     3241 cgggaactta ccacgcgggc aaagtcttcg gtttgatcat atcctgcagc taccgcctgg
     3301 tgcgtaagac ccgcactccc caccagggcg cagaagatcc aacggggaaa ggcacggctt
     3361 gataacatac aaggtctcca aagtgaggta ggggaattca ggctaggctt tgatctctga
     3421 atcctagctg aagcgcctgc tacgataaca attttccgtt caaggttcct ttaagcgatg
     3481 caaatactgt tgatcgaaga cgacaaatcc ctcgccaccg gcatgcagga agccttaaaa
     3541 aatgcgggtt tggtggttaa ttgggtggat cgcgggcagg aggcgctgca gagtttagcg
     3601 gtgcaagtgc ccgacatcgt tattttggat ctaggcctgc cggacatgga tggcttaagc
     3661 ctgctcaaaa cactgcgcag acagcacaaa caagtgcagg tgttgattct tactgcgcgg
     3721 gattctgtgg tggataaggt ggcagggctt gatgtggggg cggatgatta cctcaccaag
     3781 ccgttcgatt tggatgagct gttggcccgc ttgcgggcgt ttgagcgcag gctgggcacg
     3841 gccagtagcc acctgattgt gcacgggcgt attacgctaa acctgcgcga tcgcatcgcc
     3901 ctggtggatg atgctgaact cagtctctcg aagcgggaat ttatgttgct caaagccctg
     3961 atggagcagc aaaaccgcgt tcatacgaag gaatcattgg aggccaaact ttacagctgg
     4021 ggggaggagg tagtgagtaa tacaatcgag gtgcacatcc accatctgcg caaaaagttg
     4081 cctgaaaaca gcattcaaac cctgcgcggc gtcggctatc ggctcacaaa aattgataca
     4141 gcaggctagg acgtggggtc aattcggctc ttccttatat tgctgcttat cagtgtggtc
     4201 actattgcca actttactgc ggcggtgcgc ggttatgtgg gcagtatgca ggaggccgag
     4261 gcattttttg acgagcggtt ggaggcccag ctggaatata ttcacgccag cctcggcctt
     4321 gcgctgcgcc agcccggcgt acagacgccg ttaaagattg tgcccagctt atccctacat
     4381 aggcctcttc cacttaacta ccacatagaa tttcaagtct ttcaccccgt caatgggctg
     4441 gtggcccgct cggaagattt tccggtacag gccttagtgg tgtggcagga ggggttttcg
     4501 gagtttgagt acgaaggggt gcgctggcgg gggcgcattc actgggatga acccttaggg
     4561 ctgtggtatt tggtggcgga gcgttttgat cagcgctaca gcctggccga at
//
LOCUS       DAOQGS010000332         4606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9896_length_4606_cov_4.834981, whole genome shotgun sequence.
ACCESSION   DAOQGS010000332 DAOQGS010000000
VERSION     DAOQGS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9896_length_4606_cov_4.834981"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..321)
                     /locus_tag="PKZ52_12350"
     CDS             complement(145..321)
                     /locus_tag="PKZ52_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HNG60607.1"
                     /translation="MPNEPAQFTLLDDEVLAEIKQQLALLRYGSLEISVHNGQIVQLE
                     RREKKRWGKSENAK"
     gene            565..1128
                     /locus_tag="PKZ52_12355"
     CDS             565..1128
                     /locus_tag="PKZ52_12355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG60608.1"
                     /translation="MLTSTPLRDPLIQALAGYPALIVPGLNNSDAEHWQSRWQARLPH
                     STRIELDDWATPNLDKWCSAIDRALARTHGPQVLIAHSFGTLACARMAQLLAEKIHGL
                     FLVAPADPDKFAIADQLPKQPLGVKTHVIASSNDPWMRAEKAAHWAAQWGADFLVLKD
                     VGHINSASNLAEWPLGVEQLQRLMDGI"
     gene            1890..3110
                     /locus_tag="PKZ52_12360"
     CDS             1890..3110
                     /locus_tag="PKZ52_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNG60609.1"
                     /translation="MGTILNMTAQLKPSPAAISRTEFTVLIALLMSIIAISIDALLPA
                     LGIIRSDLKLLDANQVQLLITAIFLGMTFGQLISGPISDALGRKPILYFGLALYFIGS
                     LVCYYGQDLTHLLIGRLIQGLGVSGPYVAAMSIVRDKYTGEQMAKIMSIVMLIFMGVP
                     AIAPSLGQGILLLADWRSIFIFYIGYALVIGSWIFLRLEETLAKNNRIRLSASSFSAG
                     FKEVATNKISMGYTLCMGCIFGSLIGYLNSSQQIFQDMFATGRLFTLYFGVLALIFGA
                     ASLGNAYLVERVGMRRLCDLALRGMIASAVIFLAVQYCVAVNLAMFMLFAGINFLCFG
                     FIFGNSNAIAMEPMGHIAGIASAIIGAVSSAIALILGTCIGLAYNNTLLPVTLGFLLL
                     CSIAWLAMKFANRP"
     gene            3495..>4606
                     /locus_tag="PKZ52_12365"
     CDS             3495..>4606
                     /locus_tag="PKZ52_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNG60610.1"
                     /translation="MNRAAAKTHTSLSVRIILGVVLLIFLATVVQIYLSNQLARQALL
                     AELQAQLETKAEYNNKELRNKIDRVNRDVLYLSHLPSLDGLVRAIEHNGFDDVEQQST
                     GILTKRLQGRFASFLDANPEYFQALFIGLADDGRELIRVRSAGGIIDAVPAEQLQQMK
                     QRDYFVASQHLKKGEVYNSDIHLHQEQGELERPLRRTLSAATPVYSESGRLFGMLVIN
                     MDIGSTLDKLSADNLGYISTYMFTQRGDYIIHPNTEKTFAIDLGNSFSWQQDIERIAG
                     RAINAQDTALISLPPHHLTAFTLSGQALHGVQTDLPLDEQRFLSLIYAVEHDYLSTRN
                     RGVNKIILISASTIACLIAVLIYGYVRRLIAPLIQL"
BASE COUNT         1097 a         1108 c         1108 g         1293 t
ORIGIN      
        1 taaaatgctt ttaatgcgaa atgggaagat cgtttgaccg ccccatgggt gcgaaatgac
       61 atgcaaaact ccagataaaa aggcatatca cttccggcgg tccggtcagt gcttggtttt
      121 tttgttttaa atggttagcg ctggctactt agcgttttcg ctttttcccc agcgtttttt
      181 ctcccgtcgt tccaattgca ctatttggcc attgtgaacg gatatttcca acgagccgta
      241 gcgaaggagt gccaactgct gtttgatttc ggcgagcact tcgtcgtcta gcaaagtaaa
      301 ttgcgctggt tcgttgggca tttggctacc taatgtgacg ttggtgagcg gtataggtcg
      361 ggatgggcgc catagtaaag gggttgttta tgaagtaaaa atactcattt tttctgagct
      421 tataaccttt cgtttggatt gctgcggcgc cgctgccgtc ataatccggc ctgttgtagg
      481 ttataagctc agaaaaaatc ggtacttcag ccgccgctga aagatgcgta ccctgctggc
      541 cagtaccgat caaaaggccc tcagatgctg acctccaccc cgcttcgtga tccattaatc
      601 caggccctag ccgggtaccc ggcgctcata gtgcccggcc ttaacaatag cgatgccgag
      661 cattggcaaa gccgctggca ggcgcgtctg ccgcacagca cacgtattga actggacgat
      721 tgggcgaccc ccaatttaga caaatggtgt agcgccatcg accgggcgct ggcacgcacc
      781 catgggccgc aggttttaat tgcacacagt ttcggcactt tagcctgcgc ccgtatggcg
      841 cagttgctag cggagaaaat tcacggctta tttttggtag cccctgccga tccagataaa
      901 tttgccattg ccgatcaatt gcccaagcag ccgctagggg ttaaaacaca cgtaattgcc
      961 agctctaatg atccttggat gcgcgctgaa aaagccgctc actgggccgc acaatggggt
     1021 gccgattttt tagtgttaaa ggatgtaggg cacattaatt ccgcctccaa tttagcggaa
     1081 tggccactgg gtgttgagca attacaacgc ttgatggacg ggatataaaa gttttaagta
     1141 actatttagc ataccttatg actcttttaa gctcgggcgc caaataaagg tagatttaga
     1201 ccggaggcag aatcgattcg cggtgcagca gtgatgaata caggttattt cagaccgtca
     1261 aaaagaggga cctttttgac gagcctacac ggatgtattc acggcgtgtc tgaaataacc
     1321 tgtattcatc actgcggggg agtactgagg gctcacagta ctaaagaagt aatttcctgg
     1381 tgatttcttt aagcgctaga atcacgtccc gccatactaa aatgtgtact gaatagttac
     1441 agctttaaat acctccgcgt gggctcgtaa aaaaggactc cctttttgac gctttgaaat
     1501 aacccctatt caccactact acaccaatcc tcctccattt accgcctgcc gggaacgcgc
     1561 actgggtgtg ctgggtaatt accgggaaac taaatacgca aacgcctaca agaggtgcac
     1621 ccacaggcgt tttttatgca aagatgcctg caccttagac ctgcccccat aataaagcgg
     1681 ctagggaact tctaaaaatt accgtaccgg ctctggcagc cttagattta gtcataaact
     1741 gctcatttac tcgcagtagg ctctgccttt ttgttaactt cgcctggcgg gagcttagct
     1801 gccaagattt ttcaaaggtt gcccagcgat ttaaccgcgt cgcggtcgat ttactcagcc
     1861 gctgttcgac tagcctctac acagccacta tcggaaccat tttgaatatg accgctcagc
     1921 tcaagccctc cccagccgct atttcccgca cagaattcac tgttttaata gccctcttga
     1981 tgtcgattat cgctatttcc atcgacgcct tattgcccgc cctgggcatt attcgcagcg
     2041 accttaaatt gcttgatgcc aatcaggtac agttactgat tacggctatt ttccttggta
     2101 tgacattcgg ccaattaata tccggcccca tttccgatgc cctagggcgc aaacccattt
     2161 tatactttgg attagcgctg tattttatcg gcagtttagt gtgttattac ggccaggatt
     2221 taacccacct gctgattggg cgtttgattc agggcctggg tgtttcaggg ccttatgtgg
     2281 cggccatgtc gattgtgcgg gataaataca caggcgagca aatggccaaa attatgtcca
     2341 ttgtgatgct catttttatg ggtgtgcccg ccatagcccc ctcattaggg caaggcattt
     2401 tattattagc ggattggcgc agtattttta ttttttatat aggctacgcc ttggtcattg
     2461 gcagctggat tttcctgcgt ttagaggaaa ccctagcgaa aaacaatcgc attcgtttat
     2521 cggccagcag ttttagcgct ggatttaaag aggtggcaac caacaaaatt agcatgggtt
     2581 acaccctttg tatgggctgt atttttggca gtttgatcgg ttatttaaat tcttcgcaac
     2641 agatattcca ggatatgttt gccactggca ggttatttac cctgtatttt ggtgtattgg
     2701 cgctgatttt tggcgccgct tcattgggta atgcctatct ggtggagcgt gtgggtatgc
     2761 gccgtttatg cgatctcgcc ctgcgcggga tgattgccag cgctgtcatt tttttagccg
     2821 tgcagtattg tgttgccgta aatttagcca tgtttatgct ttttgccggt attaattttt
     2881 tgtgtttcgg ctttattttt ggcaatagca acgccatcgc catggagcct atggggcata
     2941 tagccggtat tgcctcggcg attatcggcg cggtgtcatc ggctattgca ttaattttag
     3001 gcacctgcat tggccttgct tataacaata ccctgctgcc ggtcaccctc ggctttttgt
     3061 tgttgtgttc tatagcctgg ctggcgatga agtttgccaa tcgtccctag cgcccgctac
     3121 ctcagcgcat aggcgtttgg gcaagcgttt acagcgcgcc aagtgcgcag cccatgggcc
     3181 aatcggtgag ggaagattag cgggtgaatt acagcggcag cgtgctatta ttaaaactta
     3241 cgggcagccc gtctgtattc ctaacttaat tgttataact acctgccatc ttatttaggc
     3301 aagctctgtt cgtcggcgac gcctattggc ttagcagtga aagagggggg gcttgggtgg
     3361 gcagatattt taagcatccg gtcttatttt aatttggcgg ttttgcgcaa aaaaccttat
     3421 aaaaatagct gtaaataacc actgcacaca accctaaaca gtagtgctaa aaagccttcg
     3481 caactggata gcctatgaat cgagccgccg ccaagacgca cacatcgctg agcgttcgca
     3541 tcatcttagg tgtggtgctg ttaatatttt tggccacggt tgtgcaaatt tatttgtcca
     3601 atcaattggc ccgccaagcg ctgctggcgg agctgcaagc gcagctggaa accaaagccg
     3661 aatacaacaa taaagaattg cgcaataaaa ttgatcgcgt aaatcgcgac gtgttgtatt
     3721 tatcccattt gccttcccta gatggattgg tgcgcgccat tgaacacaat ggctttgacg
     3781 atgtggaaca acaaagtaca ggcattttaa ccaaacgctt gcaagggcgt tttgcttcgt
     3841 ttctcgatgc caatcccgaa tattttcaag ccttgtttat tggccttgcc gatgatggcc
     3901 gggaattaat tcgtgtgcgc tccgctgggg gcataattga tgcagtgcct gccgaacaat
     3961 tacagcagat gaaacagcgc gattattttg ttgccagcca acatcttaaa aaaggtgagg
     4021 tttacaactc tgatatacat ctgcatcagg agcagggcga acttgagcgc ccattgcgcc
     4081 gcacattaag tgcagccaca cccgtttaca gcgaaagcgg ccgcctgttt ggcatgctgg
     4141 taataaatat ggatataggt tcgacactgg ataaattgtc ggcggataac ctaggctata
     4201 tatccactta tatgttcaca caacggggcg attatatcat tcaccctaat accgaaaaaa
     4261 cctttgctat tgatctgggt aattcattta gctggcagca ggatattgaa cgcattgccg
     4321 gcagggcaat taacgcgcag gatacggcct taatcagcct gccgccacat catctcaccg
     4381 cgtttaccct atctgggcaa gccctgcacg gcgtacaaac ggatctgccg ctggatgaac
     4441 agcgttttct ttcgttaatt tatgccgtcg aacacgacta cctcagcacg cgcaatcgcg
     4501 gcgtcaataa aattatttta atcagtgcct ccactatcgc ctgcctaatt gcggtactaa
     4561 tttatggtta tgtacgcagg ctgatagcac ccttaataca attaag
//
LOCUS       DAOQGS010000333         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9941_length_4596_cov_3.659987, whole genome shotgun sequence.
ACCESSION   DAOQGS010000333 DAOQGS010000000
VERSION     DAOQGS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_4596_cov_3.659987"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="PKZ52_12370"
     CDS             <1..2073
                     /locus_tag="PKZ52_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG60611.1"
                     /translation="DQTESGDPAVAFHIDGIYSARSQGATALMYDLEASEILRGPQGT
                     LFGRNSTGGVINLHTAKPQDKFAASLEHTIGSYDRAATRAMLNVPVNDIWALRFAAAT
                     DNADGVVDFAPSSNLGEKYGSTDLTSYRLSSLLKPNDELQWLLTFENFNDKGAGHIPT
                     LTGGSDRSAYIQVPGMNDYEVNSLRTRLDYSFANGITLSYIGGYTESTRVSRWDRSWL
                     PGNYEWGGCIECNHDATQHEIQIKNSDDADWKWMLGLFNFRENNLTHFDIVHPNRDTR
                     PAGNSNPIYWETYRQPDRGLDSNSIFGQTTYSFTEALRGTLGARYTSDERWDKGGRNI
                     KCPSDPNTATPGIADNRQLAAAGQCWVDNINDTSPDWSKVTGMGRIEFDLNADDLIYG
                     SVATGFKSGTIQDGVGYTGATPMTPAALAAVIATNNNEAAGTQAYINPEENINYELGF
                     KGLFLEQRLQLFASLFSTRYTDLQVTSNITTATGADLLRKTNAGKATINGLELEANWL
                     VGEQGRLEGSLALLDAKYDEFYTVDSSFGADGVAFNPSANNPKFPTLLNFSGNQLVQA
                     PDVAFTLSYRHDFTLNGGATLTPRLRSTYSSEVWFDPANRGDRPQGFKNLPYAADLDR
                     QDAYVKWDTSLQFKPASEDYLLEAFVDNLTDEEIKNDQGRWNSRAQPNFMWAPGRTFG
                     LRMKVDFQ"
     gene            complement(2212..3345)
                     /locus_tag="PKZ52_12375"
     CDS             complement(2212..3345)
                     /locus_tag="PKZ52_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60612.1"
                     /translation="MHNGRPTVLFVDDSPEDLRLLAGVLVGEFDVVTAESGQQALELC
                     QSRPASVAVLDVDMPGMDGYATCCALRARHPNMQILFLSSHDGLDEILRGYDAGGIDY
                     ILKPFSAEILRGKIGGALALASNLTKLELEKNEASGAFMAAVASMGDLGVVIHFLRES
                     FQVSVSGDLARLLFDCMESYQLQSCVQLRCQFGCENYSSTGPISQLEIELLSRVAQTE
                     TRFLARGRRIFINFPCVTLLIKNAPQDENKLGRLKDALAIMLEGLNEKQLSIEKSQQI
                     LGMLRSNSRSSLLLLQELEQIVLDIEERFGELRHNNINLIDRLAEALREEFFHLAITD
                     KDEQKLMALVEAAKQDANSLFIDAQTLGAHLNWIKEKIHSKRQ"
     gene            complement(3495..>4596)
                     /locus_tag="PKZ52_12380"
     CDS             complement(3495..>4596)
                     /locus_tag="PKZ52_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNG60613.1"
                     /translation="GVKGISLFLVPKYLPDAQGGCGPKNALSCGALEKKMGIKACATC
                     VMNFDGAKAWLIGEPHQGLACMFTMMNYERLVVGIQGLGAAHGSFIQALDYANTRLQS
                     RSPTGIKNPAGPADPIIEHPDVRRMLLTMRALNEAGRAFYLYVAMALDGAKYAEPQEA
                     RRYADTVALLTPIAKAFITDKAFDTAVLGQQVFGGHGYIREWGQEQYVRDIRITQIYE
                     GTNGIQAMDLLGRKILASQGQLLAPWLAEMQAFVLTTELPAALRQAFSDALARLEGAT
                     EWVLSRAKTDPAVVGNCAVAYLHLAGLVAYGYMWLRMSAAAAGREGDFYREKQQLAAF
                     YCQHLLPQSLALHGQIIATDNPWAVGFNGVER"
BASE COUNT         1084 a         1288 c         1172 g         1052 t
ORIGIN      
        1 gatcaaaccg aaagcggcga cccggcggtg gcgtttcata ttgatggtat ttattcggcc
       61 cgctcccagg gcgccaccgc cctgatgtac gatttagaag ccagtgaaat tctacgcggc
      121 ccccagggca cattatttgg ccgcaactcc accggcggcg tgattaattt acacaccgcc
      181 aaaccccaag ataaatttgc cgcaagcctt gaacacacga ttggcagtta tgatcgcgcc
      241 gccacccgcg ccatgcttaa tgtgccagta aatgatatct gggcgctgcg ttttgccgcc
      301 gctaccgata atgctgacgg tgtagtcgat tttgccccca gctctaatct gggtgaaaaa
      361 tacggctcca ccgatttaac ctcctaccgg ttgagttcgt tattaaaacc caatgacgaa
      421 ttgcaatggt tattaacctt cgaaaatttt aacgataagg gtgcaggcca tattcccacc
      481 ctcactggcg gcagtgaccg cagtgcctat attcaagtgc cgggcatgaa tgattacgaa
      541 gtgaattccc tgcgcacacg cttggattat tccttcgcca acggcattac cctcagttat
      601 ataggcggtt acaccgaatc gacccgcgtt tcccgctggg atcgcagctg gttgcccggc
      661 aattacgaat ggggcggttg tattgaatgt aatcacgatg ctacccagca cgaaattcaa
      721 attaaaaaca gcgacgatgc cgattggaaa tggatgctgg ggttatttaa tttccgcgaa
      781 aataatttaa cccactttga catagtgcat cccaaccgag atacgcgccc ggctggcaac
      841 agcaacccca tttattggga aacctaccgc cagccggatc gcggtttgga ttccaacagt
      901 atttttggcc aaaccactta cagctttacc gaagcgctgc ggggcacctt gggtgcgcgc
      961 tataccagcg atgagcgctg ggataagggc ggccgcaata ttaaatgccc ctccgacccc
     1021 aataccgcca ccccaggcat tgccgataat cgccaactcg ccgctgcagg ccaatgctgg
     1081 gtggataata ttaatgacac ctcgccggat tggagcaaag ttaccggcat ggggcgcatt
     1141 gaatttgatt taaatgccga tgatttaatt tacggctctg tggccacagg ttttaaatcc
     1201 ggcaccattc aagacggtgt tggctacacg ggtgcaaccc ctatgacgcc ggcggcctta
     1261 gccgcagtta ttgccactaa taataacgaa gcggcgggca cccaggcgta tattaatccg
     1321 gaagaaaata ttaactacga gttaggtttt aaaggtttat ttttagagca gcgcctgcag
     1381 ttattcgcct cgttattttc cacccgctac acggatttac aagtcaccag taatatcacc
     1441 actgccacag gtgcggattt actgcgcaaa accaacgcgg gtaaagccac tattaatggc
     1501 ttggagcttg aggctaattg gttggtgggt gaacagggcc gtttggaagg ctcactggcg
     1561 ctcttggatg ccaaatatga tgaattttat accgtagatt cgtccttcgg tgccgatggt
     1621 gtggccttta accccagcgc caataacccg aaattcccca ccctgttaaa tttcagcggc
     1681 aaccagttgg tgcaggcacc ggatgtggca tttaccttaa gttatcgcca cgactttacc
     1741 ctcaacggtg gcgccaccct aaccccgcgc ctgcgcagca cctattcatc ggaagtttgg
     1801 tttgaccctg ccaaccgggg cgatcgcccg cagggcttta aaaatctgcc ctatgcggcg
     1861 gatttggacc gccaggatgc ctatgtgaag tgggatacca gcctgcaatt taagcccgcg
     1921 agcgaagatt atttattgga agcatttgtg gataatctca ccgatgaaga gattaagaat
     1981 gaccaagggc gttggaatag ccgggcacaa ccgaacttta tgtgggcgcc cgggcgcacc
     2041 tttggcctgc gcatgaaggt ggattttcaa taacagccgc ccataaaaaa accgcgctta
     2101 gcgcggtttt tttatgggct tattaacccg gcaccaaatg ccgccgggtt aataactttt
     2161 ttaaatagat taggtgcgaa tgcgcatgca attgtgcgtg aataaactgg actactgccg
     2221 cttactgtga attttttctt ttatccaatt gagatgggca cctaaagtct gggcatcaat
     2281 aaataaacta ttggcgtcct gcttcgcagc ctccaccagg gccatcaatt tttgctcatc
     2341 cttgtccgta atcgccaagt gaaaaaattc ctcgcgcagg gcttcggcta acctatcaat
     2401 caaattgata ttgttatggc gcagctcgcc aaaacgctct tctatatcca acactatctg
     2461 ctctagctct tgcaataata aaaggcttga gcggctgttg gagcgcaaca tgccgagaat
     2521 ctgctggctt ttttcaatgc ttaattgttt ctcgttcagc ccctctagca taatcgccag
     2581 ggcatctttt aagcggccca atttattttc atcctggggg gcatttttaa tcagtagggt
     2641 cacgcacggg aagttaataa aaatgcgccg cccgcgggcc aaaaagcgcg tttccgtttg
     2701 ggcgacgcgg cttaataatt caatttccaa ttgcgagata gggcctgtac tgctgtaatt
     2761 ttcgcagcca aactgacagc gcaactgcac gcaggattgc agttgatagg attccatgca
     2821 gtcaaataac aaccgcgcca aatccccgct gacgctgacc tgaaaactct cccgcaaaaa
     2881 atgaataaca acccctaaat cccccatgga ggccacggcg gccataaacg cgccggaggc
     2941 ttcgtttttt tccagttcta atttagtgag gttggacgcc agcgccaggg cgccgccgat
     3001 cttgccgcgc agaatttctg cggaaaaggg tttgagaata tagtcgatac cgccggcgtc
     3061 atagccgcgc agaatttcat ccaaaccgtc gtggctggag agaaataaaa tttgcatatt
     3121 cgggtggcgc gcgcgcaggg cgcagcaagt ggcatagccg tccatgccgg gcatatccac
     3181 atccaaaact gccactgatg cagggcggct ttggcacagc tccagcgctt gttggccgct
     3241 ctcggccgtc accacgtcaa actcgcccac caaaacgccc gccaatagac gcaagtcctc
     3301 aggagaatcg tctacaaaca atacggtggg tcgtccatta tgcatagcgt tcggccatcg
     3361 ggttagggac caagcgcagc caggtccgca gggtggttag agagcctgtg atttaattgg
     3421 cgttagccgc gcgcgcggcc aactcttatc cagtctagac cggcaatagc catcgcgctc
     3481 taaacctgcc cgccttaacg ctcgacgcca ttaaaaccca ccgcccaggg gttgtccgtg
     3541 gcgataattt gcccgtgtaa ggccaggctc tggggcagca ggtgttggca gtaaaaggcc
     3601 gccagctgtt gtttttcccg gtaaaaatcc ccctcccgcc cggcggctgc cgcactcata
     3661 cgcagccaca tatagccata ggccaccagg cccgccaggt gcaaataggc caccgcgcaa
     3721 ttgcccacca ccgcagggtc cgttttggcc cggcttaaca cccactcggt ggcgccctcc
     3781 aagcgcgcca gggcatcgct aaatgcctgc cgcagtgccg ctggcagctc ggtagtgagc
     3841 acaaacgcct gcatttcggc aagccaaggc gcaagcaatt gcccttggct cgccaggatt
     3901 ttacggccta atagatccat ggcctgaatg ccgttggtgc cctcgtaaat ttgcgtgatg
     3961 cggatatccc gcacgtattg ctcctggccc cactcgcgga tataaccgtg gccgccgaac
     4021 acctgctggc ccagcacggc ggtgtcaaag gctttgtcgg taataaacgc tttggcgatg
     4081 ggcgtaagca gcgccacagt atcagcatag cggcgggcct cttggggttc ggcgtattta
     4141 gcgccgtcca gggccatggc cacatagagg taaaaagccc gcccggcttc attcagcgcc
     4201 cgcatagtaa gcagcatgcg gcgcacatcc gggtgttcga taatcgggtc ggcgggcccc
     4261 gccgggtttt taatgccagt ggggctgcgg ctttgcaggc gcgtgttggc atagtccagc
     4321 gcctggataa agctgccgtg ggcggcgccc aggccctgaa tacccaccac caagcgctcg
     4381 taattcatca tggtgaacat acaggccagg ccctgatggg gctcgccgat taaccacgcc
     4441 ttggcgccat caaaattcat cacacaggtg gcgcaggctt tgatgcccat ttttttctcc
     4501 aacgcgccgc agctgagggc atttttaggg ccgcagccgc cctgtgcatc cggtaaatac
     4561 ttgggcacca aaaataagga aatccctttg acgccc
//
LOCUS       DAOQGS010000334         4595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_9943_length_4595_cov_3.461454, whole genome shotgun sequence.
ACCESSION   DAOQGS010000334 DAOQGS010000000
VERSION     DAOQGS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9943_length_4595_cov_3.461454"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1907
                     /gene="murJ"
                     /locus_tag="PKZ52_12385"
     CDS             369..1907
                     /gene="murJ"
                     /locus_tag="PKZ52_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNG60614.1"
                     /translation="MRSSALVGSMTFISRILGLARDVVMARYLGADGAADAFYVAFRI
                     PNMFRRFFAEGAFSQAFVPVLSEYRAGGNAQAVRYLIDRVAGCLGLSLLLLCSVVVVA
                     APYLMVVFAPGFAEGSERYNLAVDLIRIMFPYLFFISLAGFIGSILNSYDKFAIPAFT
                     PVFLNIILIAAAVGFSSESMSPAVALGWGVLIAGFVQLGFQLPFVAQMGLAPKPKIEF
                     KDPAVKKVLGLMAPALFGASVSQLNILINTMIASLLPVGSVSWMSYSDRLVELPLGIF
                     AVAIATVILPVMSRQMKAQPEAATATLDWGMRSVLIIAIPACLALVVLAKPILIALFC
                     YGAYDERDALMSSYSLMTYAAGLPAFMLVKVLVSGFASRMDTKTPVKIGLFVLAANMA
                     LNAVVVWPLHHFWQIGHAGLTLATALAAMLNALWLYTALRRRHLFKPTVGWGRFIVQL
                     VVSALAMVLALMALLNISPAFIELVFWQRLAWLALMCAAGLAVYFLALFVCGIRLKDF
                     KGHF"
     gene            complement(2108..3343)
                     /locus_tag="PKZ52_12390"
     CDS             complement(2108..3343)
                     /locus_tag="PKZ52_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNG60615.1"
                     /translation="MGTLNRQILIASLMGLVLGCLLHPLPEANPWREGAIYSATLVAG
                     VFIGLLKMLLVPLIFASVCTGVAQLQHHQQGSTVWRLTLLCFIATMLIAMMLAVAASH
                     ISPAGAGLNLAMFGDAMAASTAKPTGLNDFVQQIVGSLFMNPFAAFAEGKVLAILIFA
                     LILGAAMAQLKEEAAPVLDFMQRLLALMMVILGWVMRLAPLGIFALLTKLIAVQSLPL
                     LLSLMHFIALIFITTFIHGAIILPGLVWLFAKTSPLEFLRLSLPACITAFSTSSSAAT
                     IPVTLKCAEQLSIPPALARFVIPLGATINMDGTALYEAAAAIFIAQLAGMDLTFSAQL
                     IIMATAMIAAMGAPGIPSAGMVTMILVLQAAGLPVEAIAILLPIDRILDTIRTAVNVE
                     GDLAVSMVVKHYCPETLEG"
     gene            3454..4023
                     /locus_tag="PKZ52_12395"
     CDS             3454..4023
                     /locus_tag="PKZ52_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNG60616.1"
                     /translation="MEALISSTAAVAIAEIGDKTQLLTLFLITRYRKPWPIVAGVLLA
                     TLVNHGLSAWLGSWVAGFIPQQWLPYLVAASFIAVGLWLLVPDKDEDEGDRFAQYGAF
                     MASLVLFFLAEIGDKTQIATVILAAKYSATVQVVLGTTLGMLLANVPVIIAGQWIMAR
                     IPMAAARKMAFLLFMLLAVATLIGAIWLK"
     gene            4145..>4595
                     /locus_tag="PKZ52_12400"
     CDS             4145..>4595
                     /locus_tag="PKZ52_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNG60617.1"
                     /translation="MPLRFHVLARTALFLSLSGGAWADTASLNSGNSAWVLTATALVL
                     FMTLPGLALFYGGLVRAKNVLSVLMQCFAICCVASLLWFFCAYSLSFAPGSAWLGDLS
                     KALFSGMGRESLQGDIPESLYALFQMTFAIITPALIVGAVAERMKFSS"
BASE COUNT          960 a         1061 c         1271 g         1303 t
ORIGIN      
        1 ggcggggttc atgtcggaag tcgctgcgct cttcctaccc acgccatttg gggctttccc
       61 tcaaattact atcattgatg ttgctgggga tgacaaaagc gttgatggct cttgggttgt
      121 gctaaagact taataatcta aattctttac ttaatagtcc ctgcaccgaa ggggataggc
      181 gacgcctgca caatcttcgt tacccacgga cttggctgcc tggaagcttt ctggaggacg
      241 tatatcgagg ccgcaaacca ttgctgggct aaggatctat tgcccttgtc ctgcgcccca
      301 tttcatggct caatgccgcg cttttaacgg tggaatttgt ggctcacact cagcctgcta
      361 acaaactcat gcgctccagt gctctggtgg gcagtatgac gtttatttcg cgtattttag
      421 gcctcgcccg tgatgtggtg atggcgcggt atttgggggc ggatggggcg gcggatgcgt
      481 tttatgtggc gtttcgcatt cctaatatgt ttcggcgttt ttttgccgag ggggcgtttt
      541 ctcaggcgtt tgtaccggtg ctgtcggagt atcgggccgg tggcaatgcc caggcggtgc
      601 gctatttaat tgaccgggtg gcggggtgct tggggcttag tttgttgctc ttatgcagtg
      661 tggttgtggt ggccgcacct tatttgatgg tggtgtttgc accggggttt gctgagggtt
      721 ctgagcgcta taacttggcg gtagatttaa tccgcatcat gtttccttat ttattcttta
      781 tctccctggc aggttttatc ggcagtattt taaatagcta cgataaattt gccattcccg
      841 catttacgcc ggtttttttg aatatcattt taattgccgc tgctgtgggc ttttccagtg
      901 aaagcatgtc cccggctgtt gccttgggat ggggggtatt aattgcgggg tttgtgcagt
      961 tggggtttca gttgccgttt gtggcccaaa tggggcttgc cccaaagcct aaaattgagt
     1021 ttaaagaccc ggcggttaaa aaagtattgg ggctgatggc gcctgcatta tttggcgcct
     1081 cggtaagcca gttgaatatt ttaattaata ccatgattgc ctctttgttg ccggtgggaa
     1141 gtgtcagttg gatgagttat tccgaccgtt tggtggaatt gcccttaggt atttttgctg
     1201 tagccattgc cacggtgatt ttgccagtga tgtcccggca gatgaaggcg cagccggaag
     1261 cggccaccgc tactttggat tggggtatgc gcagtgtatt aattattgca atccctgcgt
     1321 gtttggcttt ggtggtattg gccaagccta ttttaattgc gctgttttgt tacggcgcct
     1381 acgacgagcg cgacgcgctt atgtccagtt atagccttat gacctatgcg gccggtttgc
     1441 cggcatttat gttggtaaaa gtattagtga gcgggtttgc atcgcgcatg gatactaaga
     1501 ctccggtaaa aataggtctt ttcgtgctcg ctgctaatat ggcgcttaac gctgtagtgg
     1561 tgtggccatt acatcatttt tggcagattg gccatgccgg gcttaccttg gcgactgctt
     1621 tggcggctat gttgaatgcg ctttggttgt atacggcttt gcgccgccgc catttattta
     1681 agcccacggt gggctgggga cgttttattg tgcagttggt tgtttccgct ttggccatgg
     1741 tgttggcctt aatggcttta ttaaatatca gcccggcatt tattgagttg gtgttttggc
     1801 agcgtttggc ttggcttgcg ttaatgtgtg cagcgggttt ggcggtttat tttctggctt
     1861 tatttgtgtg cggtattcgc ttgaaggatt ttaaagggca tttttgattt tttaatgggg
     1921 gttgattttg gttgccaata caagaatgta aatcggtgtg gtgttggttt attttcttta
     1981 gttgcgcctg aacccctccc caaccctccc cttcaaaagg ggagggggta aagcgtctca
     2041 ttttgaatat tttttgctcc ttcagcgctt ctagtggagg ccgggatcgg agcagctagc
     2101 ccagcagtta accctccaat gtttccgggc aataatgttt taccaccata ctcaccgcta
     2161 aatccccctc cacatttacc gctgtgcgaa tggtatcgag gatgcggtcg atgggtaata
     2221 aaatggcgat ggcttccacc ggtaaacccg ccgcttgcag tactaaaatc atcgtcacca
     2281 tgcctgcact gggaataccc ggcgcgccca tggcggcaat catggcggtg gccataatta
     2341 ttaattgggc gctgaaggtt aaatccatgc ccgccaattg ggcgataaaa atagcggcgg
     2401 cggcttcata cagtgcggtg ccatccatat taatggtggc gcctaacgga ataacaaaac
     2461 gggccagggc aggggggatg gataattgct cggcgcattt taaggtgacg ggaatagtcg
     2521 ctgcgctgga actggtggaa aacgcggtaa tacaggcggg taaagagagt cttagaaatt
     2581 cgaggggtga ggttttagca aacaaccaaa ccaggcccgg caaaataatc gcgccgtgaa
     2641 taaacgtggt gataaaaatc agcgcaataa aatgcattag gcttaaaagc agtggcaggg
     2701 attgcacggc gattaattta gtgagcaggg caaaaatgcc taggggcgct aagcgcatca
     2761 cccagcctaa aatcaccatc attagggcta ataagcgctg cataaaatcc aatacgggcg
     2821 cggcttcctc ttttaattgc gccatggcgg cgcctagtat cagcgcgaaa attaaaatgg
     2881 ccagcacttt accttcggca aaggccgcaa atgggttcat aaataaactg ccaactatct
     2941 gctgaacaaa atcatttaat ccggtgggtt tggcggtgct tgccgccatg gcatcgccaa
     3001 acatggcgag gttaagacca gcgccagcag gagaaatatg gctagctgcc accgccagca
     3061 tcatggcaat taacatggtg gcaataaaac acaacagcgt taaacgccag actgtgctgc
     3121 cttgctggtg gtgttgtagc tgggcaacgc cggtgcatac ggaggcaaaa ataagcggca
     3181 ctaacaacat cttaagcagg ccgataaaaa ctccggccac cagggtggca ctataaatag
     3241 cgccctcgcg ccaggggttg gcctcgggta gtgggtgcag taaacagccg aggactaggc
     3301 ccattaagct agctataaga atttggcggt ttaatgtacc catggtgtgt gctcgtggcc
     3361 gttgtcgcgg ccacagcata agagccgcgc gcccttgggg caatcgcatt tcgcttcacc
     3421 tcattgccac ttgccctaca cacacgagaa tccatggaag ccttaatcag ttccaccgct
     3481 gccgtcgcca tcgctgaaat tggcgataaa acccaattgc tcacgctatt tttaattacc
     3541 cgctaccgca aaccctggcc aatagtggcg ggtgtgttat tggccacctt ggttaaccat
     3601 ggcttatccg cctggctggg cagttgggtg gccgggttta ttcctcaaca atggctgcct
     3661 tatttggtgg cggcgagttt tatcgcagta ggcttgtggc tgttggtgcc ggataaagac
     3721 gaagacgagg gcgatcgctt tgcccagtac ggggccttta tggcgagcct ggtgttgttt
     3781 ttcctggcgg aaattggcga taaaacccaa attgccacag ttattttggc cgctaaatat
     3841 tcggccacgg tgcaggtagt gctgggcaca accctcggca tgttattggc gaacgtgccg
     3901 gtgattattg ccggccagtg gattatggcg cgcatcccca tggcagccgc tcgcaaaatg
     3961 gcattcctcc tatttatgtt attggccgtg gccactttaa ttggcgctat ttggcttaaa
     4021 taggcctaat ccataccggt gcacagtgtt gtcataaaac caatggccgc actttttttg
     4081 cccattatta cctgctagtc tgcgcggctt ttcatttcac catgagaact caaggtcgcc
     4141 gattatgccg ctacgatttc acgttttagc cagaacagcc ttatttttaa gcctttctgg
     4201 cggtgcttgg gctgacactg ccagcctcaa ctcaggtaac agcgcctggg tattaactgc
     4261 cactgcactg gtgttattca tgacgctgcc aggccttgca cttttttatg gcggcttggt
     4321 gcgggcaaaa aacgtgcttt cggtgcttat gcagtgtttt gctatttgct gcgtggcatc
     4381 cctgctgtgg tttttctgcg cttatagttt aagttttgcc cctggctccg cctggttagg
     4441 ggatttaagt aaagccctgt ttagcggtat gggccgcgag agtttgcagg gcgatattcc
     4501 cgaatccctc tacgccctct ttcaaatgac ctttgccatc atcaccccag ccctaattgt
     4561 gggggcggtg gcagagcgga tgaaattctc cagtg
//
LOCUS       DAOQGS010000335         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10011_length_4575_cov_3.741593, whole genome shotgun sequence.
ACCESSION   DAOQGS010000335 DAOQGS010000000
VERSION     DAOQGS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10011_length_4575_cov_3.741593"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..920)
                     /locus_tag="PKZ52_12405"
     CDS             complement(189..920)
                     /locus_tag="PKZ52_12405"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNG60618.1"
                     /translation="MTAYFISDLHLSEETPQIYEAFKDFLTRLQAGDDLYILGDFFDA
                     WIGDDEDAPFALEVKETLRRLTQRGVTGYMLPGNRDFLLGETFCRETGLTLLPEEHVI
                     TLNGERVLLMHGDSLCTDDTEYMKFRQMVRNPAWQAQVLALPLAQRRAMAAQLRQQSK
                     SLNAMKAEDIMDVNQREVEARMEYWGVSRLIHGHTHRPCQHTFKIAGATAERWVLGDW
                     DQQYWWLIGNDQLTLKEACVVPSLI"
     gene            complement(923..1405)
                     /locus_tag="PKZ52_12410"
     CDS             complement(923..1405)
                     /locus_tag="PKZ52_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNG60619.1"
                     /translation="MITLHTNLGDITIELNPEKAPKTCANFKQYAEDGFYDGTIFHRV
                     INNFMIQGGGLTADMKQKSTRAPIENEADNGLPNDTGTIAMARTSDPHSATAQFFINV
                     KDNDFLNHSAKNSQGWGYCVFGKVTEGMDVVNKIKVVKTGAQDVPQATITIERVTVAG
                     "
     gene            1554..3218
                     /locus_tag="PKZ52_12415"
     CDS             1554..3218
                     /locus_tag="PKZ52_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNG60620.1"
                     /translation="MTDDNKPQNFLAQQIQQDLDNGIISTLVTRFPPEPNGYLHIGHA
                     KSICLNFGLAEEFGGQCNLRFDDTNPAKEDQEYIDAIIKDVRWLGFNWAGEVRYASAY
                     FDQLYNWALYLIEQGKAYVCHLTADEAREYRGTLKEPGKNSPYRERSVAENLALFEQM
                     RAGEFNEGESVLRAKIDMAHGNINMRDPILYRIRKQAHHQTGDKWCIYPTYDFAHGQE
                     DAIEGVTHSICTLEFADHKPLYDWFIEHLPVPHKPVQYEFGRLNLNYTVTSKRKLKQL
                     VDDGLVDGWDDPRMPTIAGLRRRGVTPAALRKFCQMIGVTRSDGVVDVAMLEHAIRDD
                     LDKNTPRAMCVLRPLKVTLLNYPADKTEVLTAPGHPHNEDLPSRSLPFSREIFIDQED
                     FREEANKKYKRLVLGKRVRLRNAYVIEAVNAVKNAAGDIIAIEANIIEETLGKDPADG
                     VKPKGVIHWVDGKEHVQFAVRLYERLFTDPSPDSGDKDFLQFINSDSLVELPACSGEP
                     GLKEAAIEQAYQFEREGYFCRDSRAAGLVFNRTISLKDALAKAADE"
     gene            3240..>4575
                     /gene="cysS"
                     /locus_tag="PKZ52_12420"
     CDS             3240..>4575
                     /gene="cysS"
                     /locus_tag="PKZ52_12420"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNG60621.1"
                     /translation="MQFSLYNTLSGEKELFKPLVAGKIGMYVCGITVYDFCHIGHARV
                     MVVFDVITRYLRARGWDLTYVRNITDIDDKIIQRAAENGESIDALTERFIAAMVEDET
                     ALGVLRPDQEPKATEHVAGIIDLIKLLEEKQFAYAASNGDVYYRVAKFADYGKLTNKK
                     LDELEIGKRIAIEEAKEDPRDFVLWKAAKAGEVSWPSPWGNGRPGWHIECSAMSKSCL
                     GLTLDIHGGGPDLPFPHHENEIAQSEAANGCQYAHYWMHAGAVRVNDEKMSKSLGNFF
                     TIRDVLSRYHPEVVRYLLLSSHYRSSINYSEESLKEARSALERFYGCLKQFGDLPVLP
                     LAEAFASEPGQAFCAAMDDDFNTRVALSVLFDLVRQINSSPDAHSAAVLKTLGSILGV
                     LQAAPEAFLQAGSTALDVAKIEALIHERAEAKGAKDFARCDEIRLELKAMGVV"
BASE COUNT         1095 a         1007 c         1253 g         1220 t
ORIGIN      
        1 ggatggtagt tcggcatcag tttttacaag tcagccaaga gaagttttta aataaatttc
       61 tagcggttaa cgtaggacat atttgcccat aacacatatt gtcaatttag gttttatgaa
      121 ctccactatt tatgaagtgt attagctcag cctcgatctg acagttaccg atttttcaat
      181 ggtacctgtc agattaggct tgggacgaca catgcttcct taagcgtcaa ttgatcgtta
      241 ccaatcaacc accaatattg ttgatcccaa tcccctagca cccatcgttc tgcagttgcg
      301 ccagcaattt taaaagtgtg ctggcacggc ctgtgggtat gcccatgaat aagcctgctc
      361 acgccccaat actccattcg tgcctcaacc tccctttgat tcacatccat aatatcctcg
      421 gccttcatag catttagcga cttgctctgc tggcgcagtt gcgccgccat ggctctgcgc
      481 tgggcgaggg gtaaggcgag tacctgtgct tgccaggccg ggttgcggac catctggcgg
      541 aatttcatgt attcggtgtc gtcggtgcag aggctgtcgc cgtgcatgag tagaacccgc
      601 tcgccgttga gggtgatgac atgttcttcg gggaggaggg tcaagccggt ttctcggcag
      661 aaggtttcgc ccaagagaaa atcccggttg cctgggagca tgtagcctgt cacgcctcgc
      721 tgggtgagtc ggcgcagagt ttcttttact tctagggcga agggggcgtc ttcgtcgtcg
      781 ccgatccagg cgtcaaaaaa atctcccagt atatacaaat catccccggc ctgcaggcgg
      841 gttaagaaat ctttgaatgc ctcatagatc tgaggggttt cttcgctaag gtgcaggtcg
      901 gagatgaaat aggctgtcat agttagcctg cgacggttac gcgctcaatg gtgatggttg
      961 cttggggtac gtcttgggcg ccggttttta ctactttgat tttattcacc acgtccatac
     1021 cttcggtcac cttgccgaat acacagtagc cccaaccttg gctgtttttg gcggagtggt
     1081 tgaggaagtc gttgtctttt acgttgataa aaaactgggc ggtggcgctg tgggggtctg
     1141 aggtgcgggc catggcgatg gtgccggtgt cgttgggtag gccgttgtcc gcttcgtttt
     1201 caatgggggc gcgggtggat ttttgtttca tgtctgcggt taagccgcca ccttggatca
     1261 tgaagttgtt gatcacgcgg tggaagatag tgccatcgta aaagccgtcc tctgcatact
     1321 gcttgaagtt ggcgcaggtt ttaggggctt tttctgggtt taattcgatg gtgatgtcgc
     1381 cgagattggt gtgtagggtg atcatagctg ctccaggatg aggtgtggcc aggctgcttg
     1441 tagtgcaggc atggcttggt gctaaaaagg ggggctataa tagctgcttt gtctttgaat
     1501 atgaaccgcg ctgaagttca cgcgcttagg acttacaccc aatggcaggc ccaatgaccg
     1561 acgataacaa gccgcaaaat tttctggcgc aacaaatcca acaggattta gacaacggca
     1621 tcatctcaac cctggttacc cgcttcccac cggagcccaa tggttattta catataggcc
     1681 acgccaaatc catttgttta aattttggtt tggcggagga gtttggtggc cagtgtaatt
     1741 tacgctttga cgacaccaac cctgctaaag aagaccaaga atatattgat gccattatca
     1801 aagatgtgcg ttggctgggt tttaactggg ccggggaagt gcgctatgcc tcagcttatt
     1861 ttgatcaatt gtacaactgg gcgttgtatt taattgagca gggcaaggcc tatgtgtgcc
     1921 atttaacggc ggatgaagcc cgtgaatatc gcggcacttt aaaagagcca ggtaaaaatt
     1981 cgccctaccg tgaacgcagt gtggcagaaa acctggccct atttgagcag atgcgggcag
     2041 gggaatttaa tgaaggggag agtgtattgc gagccaaaat tgatatggcc catggcaata
     2101 ttaatatgcg cgacccgatt ttgtatcgaa ttcgcaagca ggctcaccac caaactgggg
     2161 ataaatggtg catttaccca acctatgatt ttgcccacgg tcaggaagat gccattgagg
     2221 gggtaaccca ttctatttgc acactggaat ttgccgacca taaacccctg tacgattggt
     2281 ttattgaaca cttgcctgtg ccccataaac cagtgcaata cgaatttggc cggttaaatt
     2341 taaattacac cgttacctcg aagcgtaaat taaaacaatt ggtggatgac ggcctggtgg
     2401 atggctggga tgacccgcgt atgcccacca tagctggcct gcgccgccgg ggtgttacgc
     2461 ctgccgcgct acgtaaattt tgtcagatga tcggtgtgac ccgttcggat ggggtggtgg
     2521 atgtggccat gcttgagcac gctatccgcg atgatttgga taaaaacacc ccccgcgcca
     2581 tgtgtgtctt gcggccgcta aaggtgacct tattaaatta tccggcggat aaaaccgaag
     2641 ttttaacagc cccaggccat cctcacaatg aggatttgcc gagccgctct ttacctttta
     2701 gccgcgagat ttttattgat caggaggatt ttagggagga ggctaataaa aaatataagc
     2761 ggttggtgtt gggtaagcgc gtgcgcctgc gtaatgctta tgtaattgaa gcggtcaatg
     2821 cagttaaaaa tgccgctggg gatattatcg ccattgaggc caatattatc gaggagactc
     2881 tgggtaaaga ccctgccgat ggcgttaaac ccaaaggcgt tattcactgg gtagatggta
     2941 aagagcatgt gcaatttgcc gtgcgattat acgagcgttt atttaccgac ccatcaccag
     3001 acagcggcga taaagatttt ttacagttta taaattccga tagcctggtt gaattacccg
     3061 cgtgttcagg tgagccgggg cttaaagagg ccgccattga acaggcctat caatttgagc
     3121 gcgaggggta tttctgtcgg gacagccgtg ccgctggctt ggtgtttaac cgcaccatca
     3181 gcttaaaaga tgctttagcc aaagctgccg acgaataatt ttgagcgaga ttcagtgcca
     3241 tgcaattttc tttatacaac acactcagcg gtgaaaaaga actgtttaag cctttagttg
     3301 caggcaaaat tggcatgtat gtgtgcggca ttaccgtata tgacttttgc catattggcc
     3361 acgccagggt aatggtggta tttgatgtga ttacgcgcta tttgcgtgcg cgaggctggg
     3421 atttaaccta tgtgcgcaat attactgata tcgacgataa aattattcag cgcgcggcgg
     3481 aaaatggcga gagtattgat gccctaactg agcgttttat cgcggccatg gtggaggatg
     3541 aaaccgcctt aggcgtattg cgcccagatc aagaacccaa agccacagag cacgtggcgg
     3601 ggattatcga cctgattaaa ctcttggaag aaaaacaatt tgcctatgcg gcgagcaatg
     3661 gcgatgtgta ttaccgggtg gcaaaatttg ccgattacgg caagcttacc aataaaaaac
     3721 tcgatgaact ggaaataggt aagcgcatcg caattgagga agccaaagaa gacccacggg
     3781 attttgtgtt gtggaaggcg gccaaagcag gcgaggtgag ttggccttcg ccctggggca
     3841 atggcaggcc tggctggcat attgagtgtt cagctatgag taaaagttgt ttagggctca
     3901 ctctggatat tcacggcggc gggccggatt taccctttcc ccaccatgaa aatgaaattg
     3961 cccaaagcga agcggctaac ggctgccaat atgctcatta ctggatgcat gccggagccg
     4021 tgcgggtaaa tgatgaaaaa atgtctaagt ctttgggcaa tttttttacc attcgcgatg
     4081 tattaagccg ctaccatcca gaggtagtgc gttatttgtt attaagcagc cattaccgca
     4141 gctctattaa ttattcggaa gagagtttaa aagaagcccg cagcgcttta gagcggtttt
     4201 atggttgttt aaagcagttt ggcgacttgc ccgttctacc attagcagaa gcctttgcca
     4261 gcgagccggg ccaagccttc tgtgcggcta tggatgatga ttttaatacc cgcgtggccc
     4321 tgagtgtttt atttgatttg gtgcgacaga taaatagctc gccggatgca cattcggcgg
     4381 cagtattaaa aactctgggc agtattttgg gtgtgttgca ggcggctccc gaggcgtttt
     4441 tacaagcggg cagtacggct ttggatgtgg ccaaaattga agcgcttatt catgagcgtg
     4501 ctgaggcaaa gggcgcaaaa gattttgccc gctgcgatga aattcgccta gagctgaaag
     4561 ctatgggggt tgttt
//
LOCUS       DAOQGS010000336         4573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10019_length_4573_cov_5.224878, whole genome shotgun sequence.
ACCESSION   DAOQGS010000336 DAOQGS010000000
VERSION     DAOQGS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10019_length_4573_cov_5.224878"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..4223
                     /locus_tag="PKZ52_12425"
     CDS             48..4223
                     /locus_tag="PKZ52_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG60622.1"
                     /translation="MFKSPARLCVTLLAVFGLCLNTSWALADDYPRKVSFQNVMQSED
                     MALGEVEAILQDHEGFMWFGGRNALLRYDGAEFLNITIASDAGQQAEFRNVNQAIDLF
                     EDSRKQLWVATRSGLYIYNREREYLTAAKDAKTQQALFAKNVVSSIDESPGGEILLAT
                     WNGVYVFDPHTGQYQLLTLADQAADELIHSAMYDLVVDRQQNIIWLASTKGLIKINWL
                     TKHIKHFLPDPQRPDSLSNNALKTIAQDQQGELWLGADNGLYRFNPHTEQFMNYAHNP
                     SDTRSLTDNMVRHITVAKNGWVWLACQGGLNLFDREHNNFIRFTHEEGQPRSISSSIT
                     RRVYEDHNGDIWVGTYPAGVNFYDRSSDAIRVLKPHAEPARGLLSKTVEAITEDKQGQ
                     LWIGAGGVTRYNIAEDTYEHYRIGIPGVNAQEKEIKTAEPINALTDSAGDIWFGSWLN
                     GLHLYNRQRDRFEQLPYDIQLASTAIKTARILPDNSVWSVYEDRQQNFWLGTHNAGLL
                     KFDRAAHEYRVFAPIAGDDHSLSNAVVWKTFEDSKGRFWVGTAKGLNIMDRAQGTFKR
                     YEADPNKPHQLANDSVLVIFEDHKGRIWLGTDAGLHLYREATDDFELYGLKQGFVDNG
                     IRSITEDPLGNLWLGTNNGIVMFNADTLRVQNYIRHMGENIGGTSTGASLTTAKGIVA
                     IGSKNGLFLIDVKKLQINQNPPPIAFTELRIFTQKVLPGEQDGILQRVINQTDAVTLD
                     YKKNMLSLHFAALNFRNPDKNRYAYKLEGFDDNWREVGNQRSALYTNLDAGNYVFRVR
                     AANNDGVWNEEGRSIRIIQRAPPWKTRWAYGAYGFISLSMLLLLVRAQFKKRRHLEAQ
                     SRLLELKVAERTAELNEKNKAIQAMLSNMRQGLFTLEPDGRIHPEYSRHLEELFAERE
                     LAGKNGFELLFAHSNIQADILSQLRSALESMIGEDETNYECNIHLLLNEYEINLNGAH
                     KYLSLDWNPIIEEQIVVKMMVSVRDITALRQMELEAREKKRELEIIGQLIQVPADKYR
                     VLVASCRRFIALSLAQIDATQERDDAVIAQLFRHLHTIKGNSRTYNLAYLSEAAHNAE
                     AYCGQLIQMSAMQLESQPQWDRRQLRADLERVAAVLNEYDQVFTHVLGRNASHAHGQT
                     GIWLDSRATASLTTILTCIRRGTPANIDQTQLLRLEQTMQGALHKSLADVLADIINAL
                     PSLAAQLGKATPTLNISAEAIVINRQSEEWLQNIFAHLLRNCLDHGIEPAQLRTALGK
                     PAAGTIVITANIRCEELIITLRDDGQGLDMEKLFQLGVQRNFWPPDATPTLGEIANII
                     FHAGISSKEQVTEISGRGVGMDAVKQFVSEQGGAITIHLDADPQARPVERSKIQFELI
                     IRLPAGQLIADLV"
BASE COUNT         1220 a         1018 c         1184 g         1151 t
ORIGIN      
        1 aggcgttcaa accctgtcgt aaaagaccgc taacagccag cctacctatg tttaaatctc
       61 ccgcccgatt atgtgtaacg ctattagccg tatttgggtt atgcctaaac acatcctggg
      121 ctctagccga cgactatccg cgcaaggtga gctttcagaa tgtaatgcaa agtgaggata
      181 tggctctggg agaggtggaa gccatactgc aggaccacga gggttttatg tggtttggcg
      241 ggcgcaatgc actgttgcgt tacgatgggg cggaattttt aaatatcacc atcgcaagcg
      301 atgcaggcca gcaggcagaa tttcgtaacg tcaatcaagc catcgacctg tttgaggaca
      361 gccgcaagca gctatgggtt gccacccgct caggtttgta tatatataac cgcgagcgtg
      421 aatatttaac cgcagcaaaa gacgccaaaa cacaacaggc cttatttgcc aaaaatgttg
      481 tcagcagcat tgatgaaagc cccggtggcg aaattttatt ggccacttgg aatggggtgt
      541 acgtctttga cccccatacg ggccagtatc aattgttaac acttgcggat caggcggccg
      601 atgagcttat tcacagcgcc atgtatgatc tggtggttga ccggcagcaa aatattattt
      661 ggcttgccag caccaaaggt ttaataaaaa ttaattggct tacgaagcac ataaaacact
      721 ttttgcccga cccgcaaagg cccgattctc tcagtaataa tgcgctaaaa accatcgcgc
      781 aggatcagca gggggagctt tggttgggcg ctgataatgg cctgtaccgg tttaacccgc
      841 acacagaaca atttatgaat tatgcgcaca accccagcga tacgcgcagc cttacggata
      901 atatggtgcg gcatattacg gtggctaaaa acggttgggt gtggcttgcc tgccagggcg
      961 ggcttaattt atttgatcgc gagcataata attttattcg ttttactcac gaggaggggc
     1021 agcctagatc aataagtagc agtatcaccc ggcgggtgta cgaggaccat aatggcgata
     1081 tttgggtggg cacttaccct gcgggtgtta atttttatga ccgctccagc gacgcaattc
     1141 gcgtattaaa accccatgct gagcctgcgc gcggtttatt gagtaaaacc gtagaagcca
     1201 ttacggaaga taaacagggc cagctgtgga ttggtgccgg tggggttacc cgctacaaca
     1261 ttgcagagga tacctatgag cattatcgca ttgggatacc tggcgttaat gcgcaagaaa
     1321 aagagattaa aacagcggaa ccgatcaatg cacttaccga tagtgctggc gatatttggt
     1381 ttggcagttg gctaaatggt ttgcacctgt acaaccgcca gagagatcgt tttgagcagc
     1441 tgccctatga tatacagctg gcgagtacag caattaaaac tgctcgtata ttgcctgata
     1501 attctgtgtg gtcagtgtat gaagatcgcc agcagaattt ttggctgggc acccataatg
     1561 cagggttgct gaaatttgac cgcgccgccc acgagtatcg cgtatttgca cccatcgcgg
     1621 gggatgatca ttcccttagc aacgccgtgg tgtggaaaac ctttgaggat tccaaagggc
     1681 gtttttgggt ggggacggct aaagggctca atattatgga tcgagcccaa ggtacattta
     1741 aacgctatga ggccgatccc aacaaaccgc atcaattagc caatgattca gtgctggtta
     1801 tttttgaaga tcataagggg cgcatatggt tgggcaccga tgcaggtttg catctgtatc
     1861 gcgaggcgac ggatgatttt gagctgtatg gcctaaagca ggggtttgtt gataatggca
     1921 tccgcagtat cactgaagac ccattgggca atttatggtt gggcactaac aacggtattg
     1981 ttatgtttaa tgccgacact ttgcgggtgc aaaattatat acgccatatg ggtgagaata
     2041 ttggcggcac cagcactggg gcttccctca ccaccgccaa aggcatagtc gccattggca
     2101 gcaaaaatgg cctgttttta atcgatgtga aaaaactgca aataaatcaa aatccgccgc
     2161 ctatagcctt tactgaattg cgtattttta cccaaaaagt actgcctggc gaacaggatg
     2221 ggattttaca gcgagtgatc aatcaaaccg acgctgttac cttagattac aaaaaaaaca
     2281 tgctgtccct gcattttgcc gcattaaatt ttcgcaaccc tgacaaaaat cgctatgcct
     2341 ataagttgga agggtttgat gacaattggc gagaagtggg caatcagcgc agtgcgctgt
     2401 ataccaattt agatgcgggc aattatgttt ttcgcgtgcg ggcagccaat aatgacggcg
     2461 tatggaatga ggagggccgt tccattcgca tcatacagcg cgcgcccccg tggaaaaccc
     2521 gctgggctta tggtgcctat ggcttcatat ccctgagtat gttgctgttg ctggtgcgcg
     2581 cgcaatttaa aaaacgtcgc catttggaag cacaaagccg gttgttggag ctaaaagtag
     2641 ccgaacgcac cgccgagtta aacgagaaaa acaaagcaat tcaagccatg ctcagcaata
     2701 tgcgccaggg cctatttacg cttgagcccg atgggcgtat tcacccggaa tattcccgtc
     2761 acttagaaga attattcgcc gagcgggagc tggctggtaa aaatggtttt gagttattgt
     2821 ttgcccattc aaatatccaa gccgatattc taagtcaatt gcgttccgcg cttgaatcca
     2881 tgatcggtga ggatgaaacc aattatgaat gcaacataca tttattatta aatgagtatg
     2941 aaattaatct taacggtgca cacaaatact tatccttaga ctggaacccg atcattgaag
     3001 agcaaatagt tgttaaaatg atggtgtcgg tgcgggatat taccgccttg cggcaaatgg
     3061 agttggaggc ccgtgagaaa aaacgtgagt tggagattat cggccaactg attcaggtac
     3121 ctgcggataa ataccgtgta ttggtggctt cttgccgccg ctttatcgct ttgagccttg
     3181 cgcaaattga cgcaacacag gagcgagatg atgcagttat tgctcagtta tttcgccatt
     3241 tgcacaccat aaaaggcaat agccgcacct acaatttagc ttatttaagc gaagcggccc
     3301 acaacgcaga agcctactgt gggcaattaa tccagatgtc cgctatgcag ttggaaagtc
     3361 agccgcagtg ggacagacgg caattgcgcg ctgatttaga gcgcgtagcc gccgtattaa
     3421 atgaatacga ccaggtgttt actcatgtat taggccgcaa tgccagccat gctcatgggc
     3481 aaacggggat ttggctggat agccgagcaa cggcgagcct tacaactatt ttaacctgta
     3541 tacgccgtgg cacccccgcg aatattgacc agacgcagtt gctcaggctt gagcaaacta
     3601 tgcagggtgc tctacataaa tcccttgccg acgtgttagc ggatattatt aatgcattgc
     3661 catcccttgc cgcgcaattg ggcaaggcaa ctccgacgtt gaatatcagt gcggaggcta
     3721 ttgttataaa tcgccaaagt gaagagtggc tgcagaatat ttttgctcat ctgctgcgca
     3781 attgtttgga tcatggcatc gagcctgctc agctgcgcac agccttgggt aaacctgcgg
     3841 cgggcaccat tgtaatcacc gccaatatcc gctgtgagga actgattata accctgcgcg
     3901 atgatggcca agggctggat atggaaaaac tttttcagtt aggtgtgcaa cggaattttt
     3961 ggccgccgga tgcaacgcct acactcggtg aaattgccaa tattattttt cacgccggta
     4021 tttcatcgaa agagcaggtg accgaaattt ccgggcgcgg tgtgggtatg gatgcagtga
     4081 agcagtttgt aagtgagcag ggcggggcca ttactatcca tttagacgcc gacccacagg
     4141 cgcgccctgt agagcgcagc aaaatccaat ttgaattaat aatcaggcta ccggcgggac
     4201 aattaattgc cgacttggtg taacagtctg cagtgaattc ttatatttaa attgctgtgt
     4261 atgggtgctt acctgtgact gtgtcgcagg cttgtatcta ttcagcacaa ttaattactc
     4321 tctccatggt ggcggtaaaa ataggtaggc tacagatgga attctccgta ccgcattggt
     4381 aaatacaggt tatgtcaggc cgttaaaaaa gagggacctt gttgacgagc ctagatggat
     4441 gtattcacgg cgtgtctgac gtaacctgta ttcaccgctg cggggattta ccatactagg
     4501 aaacctctga aaaatgtagg cggcgaagct ctggctaggc gaatttggca aaaaaagcgg
     4561 acgactgcat gga
//
LOCUS       DAOQGS010000337         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10041_length_4568_cov_4.338356, whole genome shotgun sequence.
ACCESSION   DAOQGS010000337 DAOQGS010000000
VERSION     DAOQGS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10041_length_4568_cov_4.338356"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKZ52_12430"
     CDS             complement(<1..1115)
                     /locus_tag="PKZ52_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNG60623.1"
                     /translation="MADCPLLIQRLTGPGRGRVLQTYCRLLCWLLIALVSPLCRAEEA
                     PMPDVRVLIDVSGSMKENDPNNLRVPAIELLCRLMPQGSRAGLWFFAQGVNPVVKLSA
                     VDNNWKQNAIRLSKTIHSQGLLTDIGAALEKAAAEPSGPGYKKHIILLTDGMVDVGTD
                     AEKNQAQWRRISSELLPKLKADGFHIHTIALSEKADKNLLEKLSLGTDAASVVALSAD
                     QLVKAFLQVFDTASPQPQLPIEGNHFDVDKSIEEITALIFRGDVAGQIKLIDPQGQEF
                     SAQTAPKNVNWYRADNYDLITLNKPATGQWTLGGSLAPGSRLTLVSDLKLAVQPLPNN
                     IFIGEGQDLSLQLQEAGKTYINPEFLKLIQFTAEIVPA"
     gene            complement(1367..2743)
                     /locus_tag="PKZ52_12435"
     CDS             complement(1367..2743)
                     /locus_tag="PKZ52_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNG60624.1"
                     /translation="MGAEVEQSEVSAHAYSEHLALVNEVSRVVAIEDIRNVQGQLLVA
                     KGGEIDNRLAEKVLRFKLLRPIEDSVAIENEFNVDSLLKRLDYFLEADPFLKFLKELM
                     PPLVIKSCCEAFCRFAVLRQKLTVLAIQLPIVFEQALFVGWFGTLVRHKAKGSPEECI
                     AAFIGGLSHDMGMLHISPAVLNKQGAYEPAEWRQMQAHPIIGAKILQVIPKLPAEVAR
                     GVIEHHENLDGTGYPRGLVGDKLGALGQTLNLLDSLFAIYRKYFKPFNRSLRDLIPII
                     QMNSHSRYGSAGAGAIMLLKKLPENKACGVPIELMPDFVAAVKVRNAYLNTNIKIIND
                     MATQIGYQNKNPRIIAIQNSIIHIAVSLVQSGIINDAYMRWLDQVLNERLEFAYREVE
                     EAFILMQEVVFHLDKLKQQLMAFIEFDTDAAKMPFLLPAMEQLIAVPMQPIPDSLKDL
                     WVGRMVVY"
     gene            complement(3386..4498)
                     /locus_tag="PKZ52_12440"
     CDS             complement(3386..4498)
                     /locus_tag="PKZ52_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG60625.1"
                     /translation="MKKLFAIFLGVLTLAVFVGTAVFLYKKNQQKPVIYETVQATKVD
                     IVKKTVATGQIVPRKEIEIKSQVSGVVEELYVQAGQLIKRGDLIAKIEIIPNMQVLSS
                     AESQMDRARLNLDNAQRDFDRQKNLFAQKLIPESEFTKYRLALDLQKEALAAAENNVA
                     LIKEGASKKTGKISNQVKATVEGMVLNVPVKEGAFIMEYNDFKDGTTIASIANMRDLI
                     FEGKVDESEVGKIKEGMELVLNVGALGAEKYRATLEYISPKGVTDQGTVKFEIRAAIT
                     LPEGSFLRAGYSANADIVLAQRNQVLAINEGNLITENGQTFVEVMTAPQVFERRAVKV
                     GISDGINIEILDGITGSDAIKNMNPAASAQGGPKPG"
BASE COUNT         1163 a         1168 c         1057 g         1180 t
ORIGIN      
        1 gcgggcacta tttctgccgt aaattgaata agctttaaaa actccgggtt gatataggtt
       61 ttacccgcct cctgtaattg caggcttaag tcctgccctt caccgataaa aatattattc
      121 ggcaagggtt gcacggcaag ttttaaatca ctcaccaggg ttaaacggct gcccggtgcc
      181 aatgagccgc ccaaagtcca ttggccggtg gccggtttat tcagcgtaat taaatcgtag
      241 ttatccgctc ggtaccaatt gacatttttc ggcgccgttt gagcactaaa ctcctggcct
      301 tgcgggtcaa ttaatttaat ttggcccgcc acatcgcctc taaaaatgag ggcggtaatt
      361 tcttcaatgc tcttatcgac atcaaaatga ttgccttcaa taggcagttg cggctgcggc
      421 gatgcagtat caaatacttg taaaaaggct ttgactaatt gatcggcaga gagcgccact
      481 acagacgcgg catctgtacc tagggatagc ttttccaaca gatttttatc ggctttttcc
      541 gatagggcta tggtgtgaat gtgaaagcca tcggccttta atttaggcaa taactcgctg
      601 ctgatgcgcc gccattgggc ctgatttttt tcggcgtcgg tgcctacatc caccatgcca
      661 tcggtgagca aaataatatg ttttttatag cctggcccgc tgggctcggc ggcggctttt
      721 tctaaggcgg cgcctatatc ggtaagcaag ccttgagagt ggatggtttt acttaaacgg
      781 atggcatttt gtttccaatt attatccact gccgatagct tcactacagg gttaacaccc
      841 tgggcaaaaa accacaggcc cgcccggctg ccctggggca ttaaacggca caataattca
      901 atggcgggca cccgcaaatt attggggtcg ttctccttca tactgccgga tacatctatc
      961 agcacccgca catcgggcat aggcgcctct tcggcacggc acaacgggct gactaaggca
     1021 atcaatagcc agcacagtag gcgacaatag gtctgcaaaa cccgtccacg gccggggccg
     1081 gtcaatcgtt ggattaacaa ggggcaatcc gccattcaat tcctcatctg gcagcgtgat
     1141 ggcgtgccca tagactacgc cttatttaaa aaggtatagc agtaaaacaa gtaaagtgga
     1201 aaccttgagg ccgccaggca agcggcattt gccttggggt gggacgctgc acgggttaat
     1261 aaggtggggc taaaacccca aagggcggga agacaccatc agctcagatg caacaggcaa
     1321 taacacgagg gttaagcata caaggccaag atctaaattc acgcgtttaa taaacaacca
     1381 tacgcccaac ccataaatcc ttaagggaat cgggaatcgg ctgcatgggc accgcgatta
     1441 actgctccat ggcgggtagt aaaaacggca tcttggcggc atccgtgtca aattctataa
     1501 atgccataag ctgctgttta agcttatcca aatgaaacac cacttcctgc ataagaataa
     1561 atgcctcctc cacttcccgg taggcaaatt ctaaacgctc attaagcacc tgatctaacc
     1621 aacgcatata ggcatcgttg ataatacccg attgcaccag ggatacagca atgtgaataa
     1681 tgctgttttg aatggcgata atgcgcggat ttttattttg gtagccaatt tgggtggcca
     1741 tgtcattaat aattttaata ttggtattta agtacgcatt gcgcaccttt acggccgcca
     1801 cgaaatccgg catcaactca ataggaacgc cacaggcttt gttttccggt agttttttta
     1861 acagcataat cgcgccagcc ccggcagagc cgtaacggct gtggctattc atttgaataa
     1921 tgggaattaa atcccgcagg gagcggttaa agggtttaaa gtatttgcgg taaatagcaa
     1981 ataaactatc caacagatta agcgtttgcc ctagggcacc caatttatcg cccaccagac
     2041 cgcgtggata gccggtgcca tccaggtttt catggtgctc tatcaccccc ctggccactt
     2101 cggcaggtaa tttagggatt acctgtaaaa tcttggcgcc aataatcggg tgcgcctgca
     2161 tttgccgcca ctctgcaggc tcataggccc cttgtttatt gagtaccgca gggctgatat
     2221 gcaacatacc catatcatgg gataaaccgc caataaaagc agcgatacat tcctcagggc
     2281 tgcctttggc cttatgccgc accagggtgc caaaccaacc tacgaataag gcctgttcaa
     2341 acacaattgg cagttgaatg gccagcacag taagtttttg gcgcaaaaca gcaaaacggc
     2401 aaaatgcctc acaacaggac ttaatcacca gggggggcat taactctttg aggaatttca
     2461 gaaaggggtc agcttcaaga aaatagtcca agcgctttaa cagcgaatcc acattaaatt
     2521 cattttcaat ggcgactgaa tcttcaatgg ggcgcagcaa cttaaaacgc agcacctttt
     2581 ccgccaggcg attgtcaatt tcaccgccct tggcaactaa cagctgccct tgcacattgc
     2641 gaatatcctc aatggcgacg acccggctca cctcattgac taatgccagg tgctcagaat
     2701 aggcgtgggc actgacttca gattgttcta cctctgcgcc catgggcgaa aacacctgtt
     2761 gtcgatttct ctcactccca agtataggca gataaaaact gagcgccagc gcgcaaaaac
     2821 gcctacatcc gacaaacgcc ccgcctaatc aactggaaaa aataagtttg tgccgaatag
     2881 cgcggcataa cggcgggttt ggcacagaag ggcgcacttt ttacggcgcc aaatttattc
     2941 tggcgatagg tgcactttta cacaacacag ggaaatgctt gggttttata actattcagc
     3001 atctttttat gtagctggag gtaattcccg cactgattag gaactatcat gtgcgtgttt
     3061 tagtgctgtg cccgcagtgg tgactacagg ttagtgcaga cactccgtaa atacatccga
     3121 gtaggctcgt caaaaaggtc cctctttttg acggtctgca ctaacctgtg gtcaccactg
     3181 ctgcaccgcg aatcaatttt gcagtatggc taacctcgag catttgccgc ccgcgcggaa
     3241 gagggtaata aggtgtgctg attagttacc aaaggtttta cgtttgtctg cacccacaaa
     3301 aaagggcctt gcgaagattt tctatcacaa ggcccgcaat aaatcggcgc ctgagccggt
     3361 ttacaggccg caagcgttat ggctgttaac caggttttgg gccgccttgc gcgctggccg
     3421 cagggttcat atttttgatg gcatcactgc cggtaatgcc atcaagaatt tcaatgttaa
     3481 tcccatcgga tatacccact tttaccgccc ggcgctcaaa tacctggggc gccgtcataa
     3541 cctccacaaa ggtttggcca ttttccgtaa tcaaattgcc ttcgttaatc gccaatacct
     3601 gattgcgctg ggcgagcact atatctgcat tcgccgaata acccgcgcgc aaaaaactgc
     3661 cttcaggcaa agtgatggcg gcgcggattt caaatttaac cgtgccctgg tcagtcaccc
     3721 ccttgggtga aatgtactcc aaggtggcgc ggtatttttc cgcgcccaag gcgcccacat
     3781 ttaataccag ctccatgcct tctttaattt tgcccacctc ggattcatcc accttgcctt
     3841 caaaaataag gtcgcgcata ttggcgatac tggcgatggt ggtgccgtct ttaaagtcgt
     3901 tatattccat aataaaagcc ccctccttca ctggcacatt taacaccatg ccctccacgg
     3961 tggcttttac ctggttggaa attttgccgg tttttttcga ggcgccttct ttaattaagg
     4021 ctacgttgtt ttccgccgcc gccaaggctt ctttttgcag atcaagggcc aggcggtatt
     4081 tggtgaattc cgattcggga atcaactttt gcgcgaacag gttcttctgc cggtcgaaat
     4141 cccgctgggc gttatccagg tttaaacgcg cgcggtccat ttgtgactcc gccgagctta
     4201 acacctgcat attggggata atttcaatct tggcgatcag atcaccccgt ttaatcagct
     4261 ggcccgcctg cacatataat tcctccacca caccggaaac ctgggactta atttcaattt
     4321 ccttgcgcgg cacaatctgc ccggtggcaa cggttttttt cactatatcc actttggtgg
     4381 cctgcacggt ttcatagatt accggctttt gttgattctt tttatacaaa aacaccgccg
     4441 tgcctacaaa taccgccaga gttaacacgc cgagaaaaat ggcaaaaagt tttttcataa
     4501 gggtctctat ttgtgtcttc cattaatgtg taaaaggtgg ggtttattcg tcctgcaatg
     4561 cgacaatg
//
LOCUS       DAOQGS010000338         4549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10097_length_4549_cov_4.425011, whole genome shotgun sequence.
ACCESSION   DAOQGS010000338 DAOQGS010000000
VERSION     DAOQGS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_4549_cov_4.425011"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2255)
                     /gene="tssM"
                     /locus_tag="PKZ52_12445"
     CDS             complement(<1..2255)
                     /gene="tssM"
                     /locus_tag="PKZ52_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HNG60626.1"
                     /translation="MKKFLAFLLHPIFVSFIGLTALALLIWFAGPLIKFGASNSAPLA
                     EVFPRVMMIVAMLVMMIIAILIGQLRKAKNNQNLVQGLSTPKEDPAQLAAKEQSTEEL
                     LQIGERFTQALDTLKNFKFKGLTGSKALYELPWYIIVGPPGSGKTTALINSGLEFPLA
                     DHLGRGAVAGVGGTRNCDWWFTNEAVLIDTAGRYTTQDSHRVVDAAAWEGFLELLKRH
                     RRRRPINGAIVAISLQDLLMQTEDERALQARTIRSRLDELMDRLEIRFPIYLMLTKSD
                     MVPGFSDFFAEINRDDREQVWGVSLPDATNPNQGPNFDFLHQELQKIIRRLYDRVLAR
                     MHAERDIKRRGVISAFPQQMENVKVLVEGFVRQAFMTNRFKFQPYLRGVYFTSGTQDG
                     TAIDRLMAQVASNFGFAREVSSGLNQQGKSYFLTRLFKEVIFPESEIVGANTGYETSM
                     RWVQRAAYIGMAALMVVLGLVWSGALVKHKLNVREVENHLTQFKAEAAKPAYQQLDMR
                     GSLPALNELRAAASVMDQQSLPWLANLGLYDGRVDDEANQAYDRELLRHFLAQMMTTL
                     EKTLQVGDEGSDLYNTFRVYLMFNKLDTLNKEEVQNWFKKFWETTIKTDEPKRAELEE
                     HLAHLLTLELTPVELNQAVVNHVRDILLKEPIAKRVYSRIRHTPAFARKVDMLDEMGD
                     KARQYFVINPQVREALQMPVLYTKAGYDKVDLSADSDVIKAVIEERSILAQAEAGKDV
                     LNYTPEEPAKV"
     gene            complement(2579..3496)
                     /gene="icmH"
                     /locus_tag="PKZ52_12450"
     CDS             complement(2579..3496)
                     /gene="icmH"
                     /locus_tag="PKZ52_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HNG60627.1"
                     /translation="MDEKDKTLFKQPGAGDSTVLKPTPGRKGGMDMRPPPVTPSYTPE
                     PIAAAGAAPQGRVDLSAFGPGLNPIVQAASTLLMVFDKTRGTLRHANVAGLHNSLVNE
                     IKQFENRLRDLNLKPEQAWASRYLMCTALDEAVMYTPWGGESAWGQRTLLSIFHRDTG
                     GGEKSFQMLDQLRQNPGDNRAVLELFYLVLSLGFEGKYRLMPRGSEALEALRDDLYAS
                     IRAQRGDYERSLASAWQGLGRTRRTLSHYLPTWVAASVVAALVFFGYVGFKLWLNASS
                     EPTLESLSRVAPISAPAAGSSATIPEQKP"
     gene            complement(3528..>4549)
                     /gene="tssK"
                     /locus_tag="PKZ52_12455"
     CDS             complement(3528..>4549)
                     /gene="tssK"
                     /locus_tag="PKZ52_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNG60628.1"
                     /translation="RLGAVDVLPQEGAQGLARYYPKEHKVRDNSIDSGDDHAVQVGKL
                     RLRVLLETDDLSGYTCIGIARIAETRQDKNILLDDKYIPTVLDCQAAPRLNGFIVELA
                     GMLRHRAEAIAGRLADTRRGGTAEVTDYMLLQTLNRLEPIMVHLAKVQGLHPVELFKE
                     CMGAIGELATFNTKNHRPPELPVYLHDNLQAGFEIVFAHLRQLLATIYEQTAISIGLE
                     DKGYGIRVADKYDKSLINSATFILVVRADIAEEALRSRLPQQMKIGPVENIRQLINSA
                     LNGILIKALPVAPRQIPFRAGHVYFELEQRGEIWRYMSTSGGFAMHVGGDFPGLEMEF
                     WAIRR"
BASE COUNT         1136 a         1229 c         1091 g         1093 t
ORIGIN      
        1 ctgaccttag cgggctcttc cggcgtgtaa tttaatacat ctttacccgc ttcggcctgg
       61 gctaaaatag accgctcttc aatcactgct ttgattacat ccgagtcagc ggataaatcc
      121 actttatcgt aaccggcttt ggtatataac accggcattt gcagcgcctc gcgcacctgc
      181 gggttaatca caaaatattg ccgcgcctta tcgcccattt catccagcat atccactttg
      241 cgagcaaatg cgggggtgtg gcgaatgcgg ctataaacgc gcttagcgat tggctctttt
      301 aataaaatat cccgcacatg attcaccacc gcctggttaa gctccaccgg ggttaattca
      361 agagtgagca aatgagccag gtgttcttct aattcagcgc gcttgggctc atcggtttta
      421 atagtggttt cccagaattt tttaaaccaa ttttgcactt cctctttatt gagcgtatcg
      481 agtttattaa acattaaata aacccggaag gtgttgtaca ggtcgctgcc ttcatcgcca
      541 acttgcagtg ttttttccag ggtggtcatc atctgggcta aaaagtgccg cagcaattcc
      601 cggtcgtagg cctgattggc ttcatcatcc acacggccgt cataaaggcc caggttggca
      661 agccaaggca gggactgctg atccataaca gacgccgctg ctcttaactc atttaatgca
      721 ggcaaactgc cgcgcatatc caactgttgg taggcaggct tggcggcttc ggctttaaat
      781 tgggttaagt ggttttccac ttcccgcaca tttaatttat gtttcaccaa tgcgccagac
      841 cacaccaaac ccaagaccac cattaaggcc gccataccaa tataggccgc gcgctgcacc
      901 cagcgcatgg aggtttcata gccggtgttg gcgccgacaa tttccgactc agggaaaata
      961 acttctttaa ataaacgggt aagaaaataa cttttaccct gctggttcag cccactggaa
     1021 acttcccgcg caaaaccaaa attcgacgcc acttgggcca ttaagcggtc gatggcggtg
     1081 ccatcctgag tgcccgaggt aaaatacaca ccgcgcaaat agggttggaa tttaaagcgg
     1141 ttggtcataa aggcttggcg cacaaaaccc tccaccaaga ctttgacatt ttccatctgc
     1201 tgcgggaagg cgctaattac cccgcgccgc ttaatatccc gctccgcatg catacgggcc
     1261 aaaacccggt cgtataggcg gcggataatt ttttgtaatt cctggtgcag aaaatcaaaa
     1321 tttggcccct gattcgggtt ggtggcatcc ggcagcgata ccccccacac ctgctcgcga
     1381 tcatcgcgat taatttccgc aaaaaagtcg ctaaagcccg gcaccatgtc ggatttagtg
     1441 agcattaaat aaatcgggaa gcgaatttcc aaacgatcca ttaattcatc taaacgcgag
     1501 cgaatggtgc gcgcctgcaa cgcccgctca tcttcggttt gcattaataa atcctgcaaa
     1561 ctaatcgcca ctatggcgcc attaataggc ctgcggcggc ggtggcgttt taacagctct
     1621 aaaaaacctt cccaggcagc ggcatccacc actcggtggg aatcctgagt ggtatagcgc
     1681 ccggcggtat caattaacac cgcttcgttg gtaaaccacc agtcgcaatt gcgcgtaccg
     1741 cccacaccgg ccacagcgcc gcggccgaga tgatccgcca ggggaaactc taaaccggaa
     1801 ttaattaatg ctgtagtttt acccgaaccc ggcggcccaa caatgatgta ccagggcaat
     1861 tcatacagcg ctttactgcc ggtgagacct ttaaatttaa agttttttaa ggtatccagg
     1921 gcctgggtaa aacgctcgcc aatttgcaat aattcttcgg tggattgctc tttggcggcc
     1981 agctgggcgg ggtcttcttt gggcgtggat aaaccctgca cgaggttttg attgttttta
     2041 gctttgcgca actggccgat taaaatggcg atgatcatca tcaccaacat ggccacaatc
     2101 atcatcacgc gcggaaatac ttccgccagt ggcgcgctgt tgctggcacc aaatttaatt
     2161 aaggggccag caaaccaaat taataatgcc aacgccgtaa gcccgataaa cgagacaaat
     2221 atcgggtgca gtaaaaacgc caaaaatttc ttcataccaa tggatacctg ttgtcgatcc
     2281 gcaacgaata ggcagacctt tattaacccg gcggcaaaat agatcgtcct gatctatttt
     2341 gccttcgccg cactaaacct acgaaaatgc gcggattttc ttgttttagt gcggctcgcg
     2401 gcggtttgca ccgccgaggc ggcatccttg ccgcctttat taacccggca ctaattgccg
     2461 ccgggttaat aatacgcgct aaaacctctg cgcagcagtg ttattaaagt tgtaaaagcc
     2521 tctgcctaat cggcatttta taaaaacacc cattaatcac aggctattta ggaagccgct
     2581 aaggtttttg ctctggaatt gtcgcactag aacctgccgc gggtgcacta ataggtgcaa
     2641 ctcggcttaa ggattctaaa gtgggttcgg aactagcatt taaccaaagt ttaaaaccca
     2701 cataaccgaa aaataccaaa gccgccacca cacttgccgc cacccaggtg ggtaaataat
     2761 gggacagggt gcggcgggtg cggcccagcc cctgccaggc gctcgctagg ctgcgctcat
     2821 agtcaccgcg ctgggcgcga atggaggcat acaggtcatc ccgcagcgcc tctaaagcct
     2881 cgctacccct gggcattaag cggtatttgc cttcaaaacc gagacttaat actaaataaa
     2941 ataattccaa tactgcgcga ttgtcgccgg ggttctgccg cagttggtcg agcatttgaa
     3001 aggatttttc accgccgccg gtatcgcggt gaaaaatact taacaaagtg cgctgccccc
     3061 aagcgctctc gccgccccag ggggtataca tcaccgcttc atccaaggcg gtacacatta
     3121 aataacggga ggcccaggcc tgttccggtt ttaaatttaa atcccgcagg cggttttcaa
     3181 attgtttaat ttcattcacc aaagaattgt gcaaacccgc cacattggcg tggcgcagtg
     3241 tgccgcgggt tttatcaaac accattaata aagtactggc ggcctgcaca atggggttaa
     3301 ggcccggccc aaaggcgctt aaatccaccc gcccctgggg cgcggcgcca gctgctgcta
     3361 tgggctccgg tgtataactg ggcgttaccg gcggcgggcg catgtccata ccccctttgc
     3421 ggccgggggt gggttttaat accgtgctgt cgccagcgcc aggttgtttg aagagggttt
     3481 tatccttttc atccatagta gtgtctcgca aaagttagcg gcatgattta gcggcgaatg
     3541 gcccaaaatt ccatttccaa tcccgggaag tcgccaccca catgcatggc aaatccaccg
     3601 gaggtactca tataccgcca aatttcaccc cgttgctcta attcaaaata aacgtggcca
     3661 gcgcggaagg gaatttgacg gggtgcaacc ggcagcgcct taattaaaat accattgagt
     3721 gcagaattaa taagctggcg gatattttcc accgggccaa ttttcatttg ttgtggcagg
     3781 cggctgcgca gggcttcttc agcaatatcc gcccgcacaa ccaaaataaa ggtggcggaa
     3841 ttaatcagcg acttgtcgta tttgtccgcc acccggattc cataaccctt atcctcaagg
     3901 ccgatggaga tggcggtttg ttcgtaaata gtggccagca attgccgcaa atgggcaaac
     3961 acaatttcaa acccagcctg caaattatcg tgtaaataca ccggcagttc gggcgggcgg
     4021 tgatttttgg tattaaaggt ggccagctcg ccgatggcgc ccatacattc tttaaataat
     4081 tccaccgggt gtaagccctg cactttggct aagtgcacca tgataggttc aagccggttg
     4141 agggtttgca gcagcatata atcggtaact tcagcggtgc cgccgcggcg tgtatccgcc
     4201 aagcggccgg caatagcttc ggcgcggtgg cgcagcatgc ccgctaattc cacaataaaa
     4261 ccattcaaac gcggcgccgc ctggcaatcg agaacagtgg gaatatattt atcatccaac
     4321 aaaatatttt tatcctggcg agtttccgcg atacgcgcga taccgataca ggtgtaacca
     4381 cttaaatcat cggtttctaa taatacccgc agacgcaatt tacccacctg cacagcgtgg
     4441 tcatcgccgc tatcaatgga gttatcccgc accttgtgtt ctttcggata ataacgggcc
     4501 aggccctggg cgccctcttg cggcagcaca tccacagcgc ctaaccggc
//
LOCUS       DAOQGS010000339         4512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10245_length_4512_cov_3.696881, whole genome shotgun sequence.
ACCESSION   DAOQGS010000339 DAOQGS010000000
VERSION     DAOQGS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_4512_cov_3.696881"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..657
                     /locus_tag="PKZ52_12460"
     CDS             175..657
                     /locus_tag="PKZ52_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60629.1"
                     /translation="MIETLLGGLLGGAFRLAPEALKWLDRVGERRHELAMQDKALAFE
                     QLRGAQRMAEINANAEIAWNTGSLEALREAIKGQSVPTGIAWADALSTSVRPVITYAF
                     MTLYSATKAVTVMIALSTEAGWGMALEHAWSAADQALWAGILNFWFLGRTLDKMQRGV
                     "
     gene            657..1130
                     /locus_tag="PKZ52_12465"
     CDS             657..1130
                     /locus_tag="PKZ52_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNG60630.1"
                     /translation="MVAFTVPPLAIALAKRFEGFEKQVKRTTPITAVPYICPAGYWTI
                     GYGHLCTPTHPAITELEAERYLAQDLIAALTATLRLCPVLIQEPEHRLAVIVDFTFNL
                     GAGRLQTSTLRLRINQKNWVEAARELKRWVHGGGKVLPGLVLRRDMEIQYLLGNS"
     gene            complement(1264..1836)
                     /locus_tag="PKZ52_12470"
     CDS             complement(1264..1836)
                     /locus_tag="PKZ52_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60631.1"
                     /translation="MTNKDFFFGDVFWLYDSAALHLGFMNELVRKVSPEYFTSQLNRL
                     LRSNSGRIVVIDSRSCPDFDAFLRGIQKDAIGLVEIYARTDVNSAVVATLACDLFLQD
                     GIVSVFAHWCCRNEEREAEIVNTLLKPIHAAGLDKKTYLRIDNKVERALYNGDHESEI
                     NRVIRLSKTIPSIDIEAKAIELASVSVRTC"
     gene            complement(2092..4110)
                     /locus_tag="PKZ52_12475"
     CDS             complement(2092..4110)
                     /locus_tag="PKZ52_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60632.1"
                     /translation="MLLNQSVWDEIGTMKKLVTFMLFGILTFSVAGCGKGGGKEASKD
                     SSPVTLTEKGESTITVKEGDGVSLFIELDSFSSARASRLKATKINQSDVIHEDPNPGT
                     GVFANGYVQYIKFIKVKGPDIESAQTVTPYSKDKQFGISFVAPDTKGAAVVEYEYKAI
                     LVNDKGQSDESIRKITVLGVNNPPVIKLAPTAQVSPNQSVSLYATETTDVDGSVASYS
                     WVQISGTSVTLANTTQATLNFIAPNSVKSEQLVFQLTATDNLGATSTQKVTVTVSPSD
                     LPTISIRFPSVTSYTTASTISVSGYASAKNGATIKSVTVRVDNDAVLTSMKGNFWSVS
                     SFYLPTNFDTVPVVATVEDSLGKMAEARATIYRKAPIFSDIKWNKLMGLNVNPLNGGL
                     LALINDNTLFDDYELAALSFDSKLTDKSVYDFPSAAFESVPRFDGNVATAFSLTDNKY
                     YLSSTVQASGKSSSLIHLVDLSTKKMQQVYPLEINKDVSLTSISGIAVDKNSTVYVAD
                     GSQNRLYSIDPDKFGVKAQGEALFPVEKVNFISLATDFFAEKYAPYVLPNSNEMPLYG
                     VNKSTKAVEQIAGDFLGGSTAAKRFVVDEVIGTAYVLDAKGDVYRLNLKDNTRVLFAK
                     GPIGDLAYDPIDSILYFYYPDSKSIHMIDIFSGESVEVTRSKSDLLLK"
     gene            complement(4269..>4512)
                     /locus_tag="PKZ52_12480"
     CDS             complement(4269..>4512)
                     /locus_tag="PKZ52_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60633.1"
                     /translation="YNIGGKALNKPVISYTTKVYKDGVLVRARESGTFTTKFAGLKDT
                     AVNLEIAAFKWDKEDEPKDLEVLASVIVSDLKKIGD"
BASE COUNT         1207 a         1090 c          999 g         1216 t
ORIGIN      
        1 gctggaaaac ggccacgctg cccgcgacat ccgcgatctg cgggatttag tggaggcctg
       61 gcgggaggcc cgccataccg cctggcaaac cttcatcaaa atcatcacca ccagcatcct
      121 catggcgttg atcctcggcg ccgcctttaa actcaaaatc ctcggagacg gccaatgatc
      181 gagacacttt taggaggttt attgggtggt gcctttcgcc tcgcgcccga ggcattgaaa
      241 tggctggacc gcgtcgggga gcgccgccac gaattggcca tgcaggataa agcgctggcg
      301 tttgaacagc tgcgcggtgc ccagcgcatg gctgaaatca atgccaacgc ggaaatcgcc
      361 tggaatactg gaagcctcga agctctacgc gaggccatta aggggcaaag tgtaccgact
      421 ggaatcgcct gggcagacgc actctccacg agcgttaggc ctgtgattac ctatgcgttt
      481 atgacgctct acagtgcaac taaagccgtg acggtgatga tcgcactgag caccgaagcc
      541 ggttggggca tggcgcttga gcacgcctgg agtgctgcag atcaagccct ctgggcggga
      601 atactgaact tctggttttt ggggcgaacc ctcgacaaga tgcagcgggg ggtgtgatgg
      661 tcgcatttac cgtcccgcca ctggccattg ccctcgccaa acggtttgaa ggattcgaaa
      721 aacaggttaa acgcaccacc ccaatcacgg cggtgcccta tatctgccca gcgggttatt
      781 ggacgattgg ctatggccat ctgtgcacac ccactcatcc cgccatcact gaactggaag
      841 cggagcggta cctggcgcag gacttgattg ccgccctcac ggcgacgctg cgcctttgtc
      901 ccgtcctgat acaagagccg gaacaccggc tcgcggtcat cgtcgatttt acctttaacc
      961 tgggtgctgg ccgcctacaa acgtccacat tgcgtctgcg cattaatcaa aagaactggg
     1021 tggaggccgc ccgtgaactc aagcgatggg ttcacggggg tggaaaggtg ttgccggggt
     1081 tggtgctgcg tcgagacatg gagattcaat atttattagg taactcgtaa ttcattacga
     1141 tttttgattt attattgtcg caaagaagaa agcaagtcgt tcatgatttc aacagattgc
     1201 gcgagcaaat gcccttgaca taggctcgcg tcgtcgtgag gaaaagtgat ctccgtaaaa
     1261 tatttaacac gtcctaacac ttaccgacgc caattcaatt gctttagctt caatatcgat
     1321 agagggtatt gtcttcgata aacgtattac gcgatttatc tcactctcgt gatcgccatt
     1381 gtagagcgct ctttcaactt tgttatcaat acgaagatat gtttttttat ccaatccagc
     1441 cgcatgaata ggcttcaaca gtgtgttgac aatttccgct tctcgttctt cattcctgca
     1501 gcaccagtgc gcgaaaacac tgacaatacc gtcctgtaga aatagatcac atgctaaggt
     1561 tgctacgacg gcactattga catctgtcct agcgtagatt tctaccagcc cgattgcgtc
     1621 cttttggata ccccttaaaa aggcatcgaa gtcaggacaa cttctactat ctataaccac
     1681 tattcttcca ctattgctgc gcagcaacct atttagctgg gacgtaaaat attccggtga
     1741 aactttgcgc accaattcat tcataaaacc aaggtgcaat gctgcactgt catacagcca
     1801 aaacacatcg ccgaagaaaa aatctttatt tgtcatatga gcatcctaaa ccgcagctta
     1861 atatttaatg agggactttg aaaatacaaa ttcatacccc acatcacgaa aattcgcaca
     1921 ccccgaacag gtaaaaatca gatcatctct gccagtctat ttgtattgac ggcttaagcg
     1981 acacggggat gaagaatgac gtcttcccta atgaatgtag accctacgtt tgcctgaaaa
     2041 aataaaaagt gccgcagctc ttaggttccg aaactgcggc acttagattt tctattttaa
     2101 caacagatct gactttgacc gggtaacctc aacagattca ccagagaaaa tatcgatcat
     2161 atgaatcgat tttgaatcag ggtagtaaaa atacaaaata ctatcaattg gatcgtacgc
     2221 caagtcaccg ataggacctt tggcgaataa cacacgagta ttatccttga ggtttaatcg
     2281 gtatacatcc ccctttgcat caagcacata agctgtacca atcacctcgt cgaccacaaa
     2341 ccgctttgcc gccgtgctac cgccaagaaa atcacctgca atctgttcta cggccttggt
     2401 gctcttattc actccataca gaggcatttc gttgctatta gggagcacat agggagcgta
     2461 tttttcggca aaaaaatcag ttgccaggga tataaagttc actttttcaa cagggaataa
     2521 tgcttcacct tgtgctttaa ctccgaactt atcaggatcg attgaataca gcctgttctg
     2581 gcttccatct gcaacataca cggttgaatt tttatcaact gcaataccgg atattgaggt
     2641 taacgataca tccttattaa tctccaaagg gtaaacctgc tgcatcttct tggtcgataa
     2701 atcaaccaaa tgaatcaatg agcttgattt tcctgaagcc tgtactgtag aacttaagta
     2761 atatttgtta tctgtcaagc tgaatgcggt agcgacattt ccatcaaacc ttggcaccga
     2821 ttcaaaagca gcacttggaa aatcatacac gcttttgtct gtcaattttg aatcaaaact
     2881 taaggcggcg agctcgtaat catcgaacaa tgtattatca ttgattagtg ccaataaccc
     2941 accgtttaat ggatttacat ttaatcccat tagcttattc cattttatat cactgaatat
     3001 aggggctttt ctatatatgg ttgcgcgggc ctccgccatt ttgcctagag aatcttcaac
     3061 cgttgccacg acgggaacgg tatcgaaatt agtgggcagg tagaagctcg acaccgacca
     3121 aaaattgcct ttcatactgg tgagaaccgc atcattatca acccgcacag tgacagactt
     3181 tattgttgct ccatttttag cggaagcata tccggacaca ctaatggtcg aagctgtggt
     3241 gtaggatgtt acggacggga atcggattga aatagtagga agatccgacg gtgaaaccgt
     3301 tacagtcact ttttgcgtac tggttgcgcc gagattgtca gttgcagtta actgaaatac
     3361 caattgttct gacttgacag aattgggcgc aataaaattg agtgtggcct gcgtggtatt
     3421 ggcaagcgtc acacttgtac cgctaatttg tacccagcta taggaggcta cacttccatc
     3481 gacatcggtt gtctcggttg catacaggga tactgactga ttgggtgata cttgcgctgt
     3541 gggcgcaagc tttataaccg gtgggttatt gacgccaagc acggtaattt tgcgaattga
     3601 ttcgtccgac tggcctttgt cattcaccaa tatggcttta tattcatact ccacgactgc
     3661 tgcgcctttg gtatcaggtg caacaaatga aatcccaaat tgcttatcct tgctgtaggg
     3721 cgtgacggtt tgcgccgatt caatatcggg gccttttact ttaataaatt taatgtattg
     3781 aacatagcca ttagcaaaca cgcctgtacc gggatttgga tcctcatgaa taacgtcgga
     3841 ttgattgatc ttggttgcct ttagtcgtga cgctcttgcg cttgaaaagc tgtcaagctc
     3901 tataaataat gaaaccccat caccttcttt aacagttata gtactttcgc ctttttcagt
     3961 tagggtcaca ggcgaggaat cttttgaggc ttccttccca ccccctttgc cgcagccagc
     4021 tacactaaac gtaagtattc caaacagcat aaaagttact aattttttca ttgttcctat
     4081 ttcatcccaa acagattgat taagcagcaa acagtggcca cttaatacca ggttgattta
     4141 acggtgtatc agagattagc gtgacgacca taccaatagg gtaagtaaaa tatgaaatcg
     4201 aaatcacgaa cctacgtaac accacagaag aagcattggc acactgccag aggcagcctc
     4261 cttttcattt agtcaccaat ttttttgaga tcactaacaa ttacggatgc aagcacttcc
     4321 aaatcttttg gctcgtcctc tttatcccac ttaaatgcgg ctatttccaa gttaacggca
     4381 gtatctttga gcccagcgaa tttggtagtg aacgtgcctg attcgcgagc acgaaccaat
     4441 acaccgtctt tgtaaacttt tgtggtgtag gaaataactg gcttgttaag tgccttgccg
     4501 ccaatattgt at
//
LOCUS       DAOQGS010000340         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10324_length_4483_cov_4.613144, whole genome shotgun sequence.
ACCESSION   DAOQGS010000340 DAOQGS010000000
VERSION     DAOQGS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_4483_cov_4.613144"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKZ52_12485"
     CDS             <1..426
                     /locus_tag="PKZ52_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60634.1"
                     /translation="RLSNRTPFGTGSSVAQICQLPSLEDFLYYSPECFFTFKHWFDLV
                     PSLSQRSLLAALTQLPEPLQNALQAQQIGSLAEHISRQGKNAQQIRHICHTWLDGFRS
                     LKIIRHLQSQAFAPAPLLASLAKQPFGPGAVTSIGNLPI"
     gene            423..1283
                     /locus_tag="PKZ52_12490"
     CDS             423..1283
                     /locus_tag="PKZ52_12490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNG60635.1"
                     /translation="MNSLLKHSLAFLRFWTPPEKWRELVALDQDPRSYTLNRMDALRR
                     IHYTAFVAGLCLLGNYYFKNLGQVEHLLLPIITLVNPALSDLNALHQWMYYSLLREVW
                     WAADYLIFYGIIPILFIRFFLKEPIADYGWRWNNTHLHLSKYAYLLLPIMAMAVSASY
                     RSDFTDHYPFYRLCSRSGSDWLMWELCYVAQFIFLEFFFRGFLLNALRPAIGANAVWF
                     MVLPYLMIHFSKPWLEATGAIFFGLFLGILALQNRSIWGGFMVHCGLALCMDVAALIQ
                     KNALPTQWLP"
     gene            1358..1894
                     /gene="ppa"
                     /locus_tag="PKZ52_12495"
     CDS             1358..1894
                     /gene="ppa"
                     /locus_tag="PKZ52_12495"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNG60636.1"
                     /translation="MSYNLVPTGKNPPDNVNVIIEIPQGGEPVKYEVDKDSGVLFVDR
                     VLGTAMRYPCNYGYIPNTLCGDGDPADVLVVLDRPLIHGTVIACRPVGVLRMTDDGGQ
                     DSKILAVPVSKITPVYDHVKNYTDLPEILVRQVEHFFQHYKDLEKGKWVKIEGWADAE
                     AARQEIVESIANYQNSGE"
     gene            2010..2096
                     /locus_tag="PKZ52_12500"
     tRNA            2010..2096
                     /locus_tag="PKZ52_12500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2044..2046,aa:Leu,seq:caa)
     gene            2423..2719
                     /locus_tag="PKZ52_12505"
     CDS             2423..2719
                     /locus_tag="PKZ52_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60637.1"
                     /translation="MQYTQSMTDLVFAIRARLSSAQKQSIKTSNPELLGDLADIYHET
                     LDITLRAHIEHLMTHAGTTWVALLHKPSAIKPVVRLHKMPLHASAVLSSRGAVA"
     gene            3484..4146
                     /locus_tag="PKZ52_12510"
     CDS             3484..4146
                     /locus_tag="PKZ52_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60638.1"
                     /translation="MKNVKRLGLAVAIAAASVSVNASTLVFDFSGTANITFDASSHTP
                     EVASAYNAFVNFVPQLKTFSGLVVLPSFEDYLTGTHTIALNTNGLHVSLSSGFLGTLE
                     GTSAMGSVIPDDTQEGDSGFLVLSEGQVAGFEWHIGASNPIVSRFNAGLPSGLPTRLG
                     SVDVVAEGTTFSYHIGDVYFAANRSGTADITMVPEVDSSAMLLAGLGIIGAIVRRRRL
                     DK"
     gene            4296..>4483
                     /locus_tag="PKZ52_12515"
     CDS             4296..>4483
                     /locus_tag="PKZ52_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60639.1"
                     /translation="MSQVVTEKISRLSQNTRIGIGRENLHQLIEALLCPSVLVATILG
                     VCFYFDGEITTATLIASLL"
BASE COUNT         1059 a         1290 c         1020 g         1114 t
ORIGIN      
        1 cggctgtcca accgcacccc cttcggcacc ggctcatcgg tggcgcagat ctgccaacta
       61 ccgagccttg aagactttct ctactactcg ccagagtgtt tttttacctt caaacactgg
      121 tttgacctag tgccatcgct cagtcagcgg tccctgctcg cagccctcac gcaactgcct
      181 gagccgctgc aaaacgccct gcaggcccag cagattggct cccttgccga acatataagc
      241 cgccagggta aaaatgctca gcagattaga cacatctgcc acacctggct ggacgggttt
      301 cgcagcttaa aaatcatccg ccacttacaa tcccaagcct tcgcgcccgc gcctctgctc
      361 gccagcctcg ctaaacaacc ctttggccca ggcgctgtaa cctcgatagg gaatttaccc
      421 atatgaatag cctactcaaa cacagcctag cctttttgcg cttttggacc cccccggaaa
      481 aatggcgcga gctggtggcg ctcgatcaag acccgcgctc ctacaccctc aaccgcatgg
      541 acgccctgcg gcgcatccat tacaccgcgt ttgtggcggg cctgtgtttg cttggcaact
      601 actacttcaa aaatttaggc caggttgagc acctattgct gcccatcatc accctggtca
      661 acccggcgct cagcgatcta aacgcactcc accaatggat gtattacagc ctattgcgcg
      721 aagtctggtg ggcggcggat tacctgatct tctacgggat tatcccaatt ctatttattc
      781 gcttcttcct caaagagccc atcgccgact acggctggcg ctggaataac acccacctgc
      841 atctgagcaa atacgcctat ttattactcc ccatcatggc tatggcagtg agtgccagct
      901 accgcagcga ctttaccgac cactacccgt tttaccgcct gtgctcccgc agcggttcag
      961 actggttgat gtgggagctg tgttatgtgg cgcaatttat ttttctggaa tttttcttcc
     1021 gcggcttttt actcaacgcc ctacgccccg ccatcggcgc caacgccgtc tggtttatgg
     1081 tgctgcccta tttaatgatt cacttttcca aaccctggct ggaggccact ggagctatat
     1141 tttttggcct cttcctgggt attttggccc tccaaaatcg ctccatttgg ggcggtttta
     1201 tggtgcactg cggccttgcc ctgtgtatgg acgtggccgc cctaatacaa aaaaatgccc
     1261 tccccaccca gtggctaccc taagcctggc aggcgcccca ggcaaaaggg ctatgctaaa
     1321 ctcgcgcctt ttaacccctc atccacagga cttcactatg agctacaacc tggttcccac
     1381 aggcaaaaat ccccccgaca acgtcaatgt cattatcgaa atcccccagg gcggcgaacc
     1441 cgtcaaatac gaagtggata aagactccgg cgtattattt gtcgaccgcg tactgggcac
     1501 cgccatgcgc tacccctgca actacggcta tatccccaac accctctgcg gcgacggcga
     1561 cccagcggac gtattagttg tactggatcg ccccctcatt cacggcacag ttatcgcctg
     1621 ccgcccggta ggtgtattga gaatgactga cgacggcggc caagactcca aaattttggc
     1681 cgtcccagtg agcaaaatta ccccggtgta cgaccatgta aaaaactaca ccgatctgcc
     1741 ggaaatcctc gtcagacagg ttgagcattt cttccaacac tacaaggatt tggaaaaagg
     1801 caaatgggtg aaaatcgaag gctgggccga cgccgaagcc gcccgccagg aaattgtgga
     1861 atccatcgcc aactaccaaa acagcggcga ataattcctc gccccaggat gggcgcacaa
     1921 ccctttagtt aaactctgaa tcccctaaaa caggccgcag agaaaagaat tgacaatcca
     1981 ttgcgcgctg ttatgatgcg cgcctctttg ccagtgtggc gaaattggta gacgcagtgg
     2041 attcaaaatc caccgggctt acccccgtgc cggttcgagt ccggccactg gtaccacata
     2101 taaaacagca ttgataaatt aatgctgtcg atttaaaaag cccttaacaa gggctttttt
     2161 ttcgtctcgg tggctacctc atacaccact ctcctctgca cacttatcga ctaagcctac
     2221 tctagcaaca gggttttgat atgtggctca ccgctaataa ccaagtgtta gtcattttga
     2281 acagaaccca gtttattggt ctaaaaaaac tccgacgacg tcgcttctgc cctctaagat
     2341 aaatttggac aagaaattgc tcctcaacac aataactcgc cacctcggcg ctctttcaca
     2401 gcagcacgtt aggaaccccc gcatgcagta cacccaatcg atgaccgatt tagtgtttgc
     2461 cattcgcgca cgcttaagct ctgcgcagaa gcaatccatc aaaaccagca atccagaact
     2521 gctgggtgat ttggcggata tctatcacga aaccctggat attaccctgc gcgcccacat
     2581 cgaacacctg atgacccatg caggcactac ctgggtggcc ttgctgcata agccttcggc
     2641 tattaaacct gtcgtcaggc tgcacaaaat gcccctgcac gccagtgctg tgctgtcatc
     2701 ccgcggggct gtggcttaaa cgccttgcac attccgttgt tgctaatgtg cggcggcccg
     2761 aatcactcta gcggtcgcct ccgtttctaa catccttcct caactaccct ccgccgccaa
     2821 ccaaacgcct taacttaaat ggtgctttac ccgcactaga gccttattcg tgcccaattc
     2881 tgaggcagtt gttgctgtgc tgattattta accaatgtaa cagtacgatt aacaaatttg
     2941 atggttaatc gacgaatgac cataacaaaa cctcgatata aagtatgaat ttgaggcaga
     3001 atggactagc atgcggctta gtacagccac ggtgggccgc ttgggggatt cccccgacgc
     3061 ggccgccaac agagttgtag ttcagacgca gcaccaaggg gtaactcgcc accttgtcgt
     3121 ttttgctgcc aaatcgaagc acaaattcaa ggtaacactg gttttaggtg cgcatcccgg
     3181 agcagctcct gggccattgt cgggttttac caaacggtgc ctaaaaaaga cgtaggtttc
     3241 tatctaagcg tcaaggcaca aaatgtgcag tttgttgcat cgcagcttca tacaactgta
     3301 tgggatttga atagttgagt tattacacct aagcattagg tgcgaaaact cagcgcaggc
     3361 gcttaatgtt aaaattttgt tacctgtatt tcatttcctg gcagaatggg tcgctcgctg
     3421 gacaggctga acatcatccc tttggcatat ttgggtcatt tgttatttta ggagtgaagt
     3481 aatatgaaaa acgtaaaacg cctaggcttg gcggtggcga ttgctgctgc cagcgtatcg
     3541 gtaaacgcgt ctacactggt gtttgatttc tctggcacag ccaacatcac tttcgacgct
     3601 tcctctcaca cccctgaagt cgccagcgcc tacaatgcgt tcgtcaactt cgtaccacaa
     3661 ctgaaaacct tctccggcct ggtagtttta cctagctttg aagattacct gaccggtaca
     3721 cacaccattg cactcaacac caatggcctg cacgtttcac tttccagcgg gtttttaggt
     3781 accttggaag gcacttccgc aatgggcagc gtgatccctg atgacactca agaaggcgat
     3841 tctggcttct tagtgctgtc tgaaggtcag gttgcaggct ttgaatggca catcggcgcc
     3901 agcaacccga ttgtgagccg cttcaacgct ggcttgccct cgggtctacc cactcgcttg
     3961 ggtagtgttg acgtagtggc agaaggtact accttctcgt accacatcgg cgatgtttat
     4021 tttgcggcta accgcagcgg cactgcagac atcactatgg tgcctgaagt ggattcctcg
     4081 gccatgttgt tagcgggcct tggcatcatc ggcgctatag tgcgccgccg tcgcttagat
     4141 aaataagtgc accctcataa ggggggtatt aaataccccc ctttttttcg cccccgtatt
     4201 gggtgtggaa atttccgcaa tttagttgca aaaaaagatc atagcggggc gaaattagtg
     4261 ttaaatgccc tccccggagt ccgttaagat ttaccatgtc ccaagtcgtc acggaaaaaa
     4321 tttctcgttt atcgcaaaac acgcgcatag gaatcgggcg tgaaaatttg catcaactta
     4381 tcgaagcact tttgtgcccc agtgttttag ttgccactat tctgggcgtg tgcttttact
     4441 ttgatggtga aattaccacc gccacattaa ttgcatcgct act
//
LOCUS       DAOQGS010000341         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10477_length_4441_cov_4.394665, whole genome shotgun sequence.
ACCESSION   DAOQGS010000341 DAOQGS010000000
VERSION     DAOQGS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10477_length_4441_cov_4.394665"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PKZ52_12520"
     CDS             complement(<1..1280)
                     /locus_tag="PKZ52_12520"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNG60640.1"
                     /translation="MLGFLLTRHWRDTPSGMCLEFWWASEQGPVCVEVPNQEVVFFIP
                     RKQASQVQALLASLPQWRMAEVALSTFTHQPVNAIYFKQHKQARRAQGMLSDAHIDHW
                     EADIRPPERYLMERFITATALIEKSAAGNLILRAAPDNNFRPNLRCISLDIETTFDAK
                     QLYSIAVWGADIKQVWMVQPPDRSSPLISSEELPIALVTNQRQLLIEFLTWLHSYDPD
                     ILIGWNLVQFDLWVLEQIAKHEQVPWQLGRNRQACQWRQEEGEAGRRFVVIPGRQALD
                     GIELLKAANYSFSRFNLHTVSQVLLGEGKLLHSSQQAQEITDLYLNNPQQFAAYNLKD
                     CQLVWEIFLKQDLLAFAIARSQLTGLLLDRIGGSVAAFEYSYLPRLHRQGFVAPNLGE
                     LHSDIISPGGWVMDSKPGLYHNVLVLDFKSLYPS"
     gene            1585..3516
                     /locus_tag="PKZ52_12525"
     CDS             1585..3516
                     /locus_tag="PKZ52_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60641.1"
                     /translation="MKPLSIGTLLCTLSLFAGCNGQIAPGNSSGGQIKNPPPRLKLTE
                     LKFDNPWLKGCVDDEARVRKIQYSDELTHLTCRGYPKAFEGFIRKTGGADMLVDTTAG
                     LEQLTALTELDLTDNYINQIDLVKLKNLTTLKLDGNLLQNLDLSNYSALKNLSITMNP
                     LHTLKLPRTGQLESLSLGFNSAGEGLGYSTLSYYLNTNSAILKDTRFFDDKTGNALSK
                     ASFQRISNIDFAQQFHLKSFSAGNLGLGETSLAFEGFPAANNLESLDLSSNNLTGINT
                     SKLSNLKSLNLIGNTIVSLDITSNPKLEELNISHNRMSSIKALPNNKLKTLNILGTSV
                     PDLDLKTVFDSKTLSTVTMSNSAILPVGLAPNLTSLTLHSNGEFDTNRLAPARNLAEL
                     TLDSGSINNIYLNNSPFKQVNLTLHQQKSPILVSCIEEIASLNIGWANPTPSEDTIAL
                     ESTKGCEFKKISLGTYASVAKIDLTSIPSEAFEISTSTSYKNIKLPHYAKSVALSGVS
                     DLNIFSFYDFPQLESLHLNNTSFDYLSLKGASVLKELAMNSVNLNQFDSGAAPDSLTS
                     LALEQVEVQSIDITSIARNLTSLVLAGCSIDIFRSSTFSKGIALDVRATPLKGDSLTT
                     FKQVSGRDLDSNGWLKPLP"
     gene            complement(4233..>4441)
                     /locus_tag="PKZ52_12530"
     CDS             complement(4233..>4441)
                     /locus_tag="PKZ52_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNG60642.1"
                     /translation="HVAAPMPGLIISVSAMTGQKVERGDALVSIEAMKMESIIRAERA
                     GVIKNVLVKMGTVVDAKDLLVEFA"
BASE COUNT         1260 a         1116 c          952 g         1113 t
ORIGIN      
        1 atactggggt acaggctttt aaaatcaagc accaaaacat tgtggtacag accgggtttg
       61 gagtccatca cccatccgcc ggggctgata atatccgaat gtaattcacc taaattcggc
      121 gccacaaagc cctgccggtg taaacgcggc aaataactgt attcaaatgc cgccaccgag
      181 ccgccgatgc gatcaagcaa caggccagtt aattggctgc gcgctatagc aaaggctaat
      241 aaatcttgct ttaaaaaaat ctcccatacc aactggcagt cttttaaatt gtaagcggca
      301 aactgctgcg ggttatttag gtataaatcg gttatctctt gagcctgctg actgctgtgc
      361 agtaatttgc cttcacccaa cagcacctgg gacacagtat gtaaattaaa acggctaaaa
      421 ctatagttgg cagcttttaa taattcgatg ccatctaagg cttggcggcc tggaatcacc
      481 acaaaacgcc gccccgcttc gccttcctcc tgccgccact ggcaggcttg gcgattccgg
      541 ccaagttgcc agggcacttg ctcgtgtttg gcaatttgct ctaacaccca taaatcaaac
      601 tgcaccagat tccagccgat taaaatatcg gggtcgtagc tatgcagcca cgttaaaaac
      661 tcgattaata attgccgctg attagttact aatgcaatgg gcaactcctc actggatatt
      721 aggggcgagg agcgatcagg cggctgcacc atccacacct gtttgatatc cgccccccac
      781 acggctatgg aataaagctg tttagcatca aaggtggttt caatatccag ggaaatacag
      841 cgtaaattgg gccgaaaatt attatcagga gcggcccgca ggattaaatt acctgcggca
      901 gatttttcga ttagtgccgt ggcggtaata aaacgctcca ttaaataccg ctccggcggg
      961 cgaatatccg cctcccagtg gtctatatgg gcgtcgctta acataccttg cgcccggcgg
     1021 gcctgtttgt gttgtttaaa ataaatggcg ttgacgggct ggtgggtaaa agtgctcaag
     1081 gcaacttccg ccatgcgcca ctggggaagg ctggccaaca aggcctgcac ctgggaggct
     1141 tgcttgcggg gaataaaaaa caccacttcc tggttgggca cttccacaca caccggcccc
     1201 tgctcactcg cccaccaaaa ctccaggcac atgccgcttg gggtatcgcg ccagtggcgg
     1261 gtaagtagaa aacctaacat tttgaagcct tggttgattc tggtcgcaca ggggttgtaa
     1321 aggtggcggg tttgcggtga attggcaagg gtagatgggc tgaatttctc acttagcgat
     1381 tggccgggac ggttggggtg tgccacagac acgcaaccga cacaccccct ccaaaaaaat
     1441 aaatccattt ttagaatttg tgcaggtaac agcacataaa tcattagcat ctgtactgca
     1501 gcggtatttg cgagatactt cgcgctgctc gctatcaact aagattttag ctggccccct
     1561 tcaggatact ttgcggagct taaaatgaaa ccattatcca tcggcaccct actgtgcact
     1621 ttaagccttt ttgctggctg taacggccaa attgccccag gcaactccag cggcgggcaa
     1681 atcaaaaacc cacccccacg cttaaaactc actgagctta aatttgacaa cccatggctt
     1741 aagggctgtg ttgacgatga ggcacgtgtg cgaaaaattc aatatagtga tgaattaacg
     1801 cacttaacct gtcgcggcta ccccaaagca tttgaaggtt tcattcgtaa aaccggcggt
     1861 gcggatatgt tggtggatac caccgctggt ttagagcaat taacagcact aaccgaactg
     1921 gatttaactg acaactacat caaccagatt gatctggtaa aacttaaaaa ccttaccacc
     1981 ttaaaattgg atgggaattt actgcagaac cttgacctgt ctaattattc ggctctaaaa
     2041 aacctcagta ttaccatgaa ccccttacac acgttaaaac tgccccgcac aggccaattg
     2101 gaaagtctta gtcttggctt taattctgcg ggggaaggac tcgggtattc cacactttcc
     2161 tattatttga acaccaatag cgccatactc aaagacactc gctttttcga tgacaaaaca
     2221 gggaatgcat taagcaaagc cagttttcag cgtattagca atatcgattt tgcacagcaa
     2281 ttccatctta agtccttcag cgcgggcaat ttaggtctcg gtgaaaccag cctcgcgttt
     2341 gaaggtttcc cagcagcaaa caatttagaa agcctagact taagctctaa caatttaacc
     2401 ggcatcaata ccagcaagct gtccaatcta aaatcactca acttaatcgg caataccata
     2461 gttagccttg acataacttc aaaccccaaa ttagaggagt taaatattag ccacaaccgg
     2521 atgagctcca ttaaggcgct tccgaataat aaactaaaaa cccttaatat tttaggaacg
     2581 agtgtaccag accttgactt aaagaccgtt tttgacagca aaacgctctc gactgtaacc
     2641 atgtccaata gtgcaatatt gcccgtcggt ttagctccca acttaacctc attaacgctt
     2701 cactccaacg gggaattcga cactaaccga ctggctcctg cgcgcaatct tgcagaacta
     2761 acattggact ccggctccat caacaatata tatctcaata actccccctt taagcaagta
     2821 aatttaacat tacaccaaca gaaatcacca attctggttt cctgtataga agaaatagcc
     2881 tcactcaata taggctgggc caacccaacg ccaagtgaag atactatcgc acttgaaagc
     2941 accaagggat gcgaatttaa aaaaatctca cttggcactt atgcctccgt tgcaaaaata
     3001 gaccttacga gcatacctag cgaagcattt gaaatcagca ccagtaccag ctataagaac
     3061 atcaaacttc ctcactacgc caagagcgtt gcactgtctg gagtcagcga tttgaacatt
     3121 tttagctttt acgattttcc acagctggaa agtttgcatt tgaataacac ctcttttgat
     3181 tatttaagtc tcaaaggtgc atcagtatta aaagaattag ccatgaactc tgtcaacctc
     3241 aatcaatttg attcgggggc cgcaccggat tctctcacca gccttgccct agagcaggtt
     3301 gaagtgcaat ccatagatat aacttccatt gcgcgcaatt taacctcact ggttctggcg
     3361 ggttgttcaa tcgacatatt taggagcagt actttcagca aaggtattgc ccttgacgtg
     3421 cgcgccactc cacttaaagg cgacagcctt accaccttta aacaggtttc aggccgcgac
     3481 ttagacagca acggatggct aaaaccactg ccgtaatagg cgggtgcaat tattggggta
     3541 gccggtctta atctgccgag gttagccact acagtttttc aagaaaaagg ggtgtaccga
     3601 ttaaagagtg ggcgcccttt ttatttgcaa aaaattcgag aaactctgga gcggaggagt
     3661 taaatcctat ttagtcagcc gttatatcct ctgcgacgct taattttacc gattgtaacg
     3721 gcgtacacag gcatggctag gagcacttgt ttcaacaaat attgcttaaa aattgcagtg
     3781 caccaaggtg cacccaacca ttcgcattgg attgcacctt acccggcaag cccaatagtt
     3841 tggttaccca cgagattttc aacccgtgca gcacatatta acggtcaaga atctttgatc
     3901 taggtttgga taaatttttt aaaacaaacc attatcgcta atggcgggtt acacccgccg
     3961 tacgaggttt ctttggggca atgcaagatt tggagtaatt taattacggg aaaaagtagg
     4021 gtggattcgg agcaaagcga caatccaccc attagcaatt ccgatctcac cgtttacaat
     4081 aataattctc cgcggcgaga ttaatggtgg attgctgcgc gaatccgccc tacgattaat
     4141 ccaccttacg ggttgccagt gcaaattaac acttaaatcc tgaacataaa aaaggcgcac
     4201 ccaagtgcgc cttaagtaga accattcggc tgtcaggcaa actccaccaa taaatccttc
     4261 gcatccacca ccgtacccat tttcaccagc acatttttaa taacccctgc ccgctcggcg
     4321 cggataatgg attccatttt catggcctca atagatacca gtgcatcgcc gcgctcgact
     4381 ttttggccag tcatggcgga gacgctgata attaagccgg gcataggggc ggctacgtgg
     4441 t
//
LOCUS       DAOQGS010000342         4432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10514_length_4432_cov_4.417181, whole genome shotgun sequence.
ACCESSION   DAOQGS010000342 DAOQGS010000000
VERSION     DAOQGS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_4432_cov_4.417181"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1839)
                     /locus_tag="PKZ52_12535"
     CDS             complement(250..1839)
                     /locus_tag="PKZ52_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HNG60643.1"
                     /translation="MISTANITMQFGAKPLFENVSVKFNNSNRYGLIGANGCGKSTFM
                     KILGGDLVPTGGQVMLEPNVRLGKLKQDQFAYEEFTVLDAVIMGHTELWAVKAERDRI
                     YSLPEMSEEDGMKVAELEVAFAEMDGYTAEARAGELLLGLDIPIEQHTGLMSAVAPGW
                     KLRVLLAQALFSDPEVLLLDEPTNNLDINTIRWLEGILRQRNSTIIIISHDRHFLNAV
                     CTHMADLDYGEIRIYPGNYDDYMLASTQVRERLLSENAKKKAQISELQAFVSRFSANA
                     SKAKQATSRARQIEKIQLDDVKPSSRVSPYIRFTQNKKLHRQAVVLENISKGFDRTLF
                     QDFSMQVEAGERIAIIGPNGAGKTTLLRCMYGDLTPDKGTIKWAEQAEIGYYAQDHSA
                     DFSDDITLFDWMKQWTKGDEQLVRGALGRMLFSNDEVKKKVKVISGGEKGRMLFGKFS
                     LINPNVLLLDEPTNHLDMESIEALNLALENYPGTIIFVSHDREFVSSLATRIIDLQPK
                     GIVDFHGNYDDYLRSQGVYTD"
     gene            complement(1959..2435)
                     /gene="bfr"
                     /locus_tag="PKZ52_12540"
     CDS             complement(1959..2435)
                     /gene="bfr"
                     /locus_tag="PKZ52_12540"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNG60644.1"
                     /translation="MKGDPKVIAHLNIILGNELIAINQYFLHAKMYKNWGLKALAKHE
                     YHESIDEMKHADVLVDRILFLDGIPNLQDLGKLLVGETTEEMLNCDLKLENKAIPDLK
                     EAIAYCESVRDYGTRDILQSILESEEEHVDWLETQLSLINQVGLQNYLQTQMEAAS"
     gene            complement(2574..2771)
                     /locus_tag="PKZ52_12545"
     CDS             complement(2574..2771)
                     /locus_tag="PKZ52_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HNG60645.1"
                     /translation="MYVCLCQGVTDRQIRQSIEDGADTLRALRQQLGVMAQCGKCGCM
                     TKELLLQALDERQSSTYYQVA"
     gene            complement(3390..>4432)
                     /locus_tag="PKZ52_12550"
     CDS             complement(3390..>4432)
                     /locus_tag="PKZ52_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNG60646.1"
                     /translation="PKSADVSPVLSVDGYEAFIVKSALVGQDYRIRVRTLASYATNLN
                     KRYPVVIKIDGQWDFSLLTSAYNCLYFDGQMPETIFIGIDWNITDDKVQALRARDLSP
                     VALPQIPNSGQAKGFVQAIASEILPAVNNRYRTTGQEFLVGGSWGGLFVTYALMERPD
                     VFDGALAIGSSYGGADQSLQAQINALAKAQPLKGNRFYAGAGKLDPAVRGSEEYYEAL
                     KKADIKGLHYKMDFLEGFGHSGMNIPGYAAGLQFLFARPNLTITKQNLQKFAGDYAPV
                     GGKGDGLKLQVGENNLQVVLGDEVINLFALAENQFYRQGVFFNLTFEGNWVTAETFFG
                     TNRYKRIMGGGK"
BASE COUNT         1185 a         1161 c          987 g         1099 t
ORIGIN      
        1 cctcgccgca gcctacccct aatttgcagc cgccacctgc acaccaacaa aaccaaacaa
       61 atacacctta aataaggccc aatctatttg ccgtctcggc gaatattagc gctaggcgca
      121 aacagttaat cctgcgttca acctaaaaac tgcagctggc aataatttta catccgtcac
      181 tcgcgcaacg ttttaacaat atcaaccgca gctatattcc cagccaaata gccacacggc
      241 cagccgaggt tagtcggtat aaacaccctg gctgcgcagg taatcgtcgt aattgccgtg
      301 gaaatccaca atgcccttgg gctgcaaatc gataatacgg gttgccaaag atgacacaaa
      361 ttcccggtcg tggctgacga aaataatagt gcctgggtaa ttttccagcg ccaggtttaa
      421 cgcttcaatg gattccatat ccaagtggtt ggtgggctca tccagcagta aaacattagg
      481 gttaatcagg ctaaatttgc cgaataacat acggcctttt tcaccaccgg atattacctt
      541 cactttcttt ttcacttcat cgttggaaaa caacatgcgc ccgagggcac cgcgcaccaa
      601 ttgttcatca cctttggtcc actgcttcat ccaatcaaat agcgttatat cgtcactgaa
      661 atcggcgctg tgatcctgag cgtaataacc gatctccgcc tgttcggccc atttaatcgt
      721 gcccttatca ggggttaaat cgccatacat acagcgcagc agcgtggttt taccggcgcc
      781 gttggggcca ataatagcga tgcgctcacc ggcttccact tgcatactaa aatcttgaaa
      841 caaggtgcgg tcaaacccct tgctgatatt ctccagcacc accgcctggc ggtgtaattt
      901 tttattttga gtaaaacgaa tataagggct tacccggctg gaaggtttta catcatccag
      961 ctggattttt tcaatttggc gagcgcggga ggtagcctgt tttgccttag aggcattggc
     1021 agaaaagcgg ctaacaaacg cctgcagttc agaaatctgc gctttctttt tggcgttctc
     1081 acttaataaa cgctcgcgca cctgagtgga ggccaacata taatcgtcgt aattaccggg
     1141 gtaaatacga atctcgccgt agtctaaatc cgccatatgg gtacagacag cgtttaaaaa
     1201 gtggcggtcg tgggaaataa taatgatggt gctattgcgc tgacgcaaaa tgccttccaa
     1261 ccaacggatg gtattaatat ccaagttgtt ggtaggctca tccagcaata acacctccgg
     1321 gtcggaaaat aacgcctgcg ccaacaacac tcgcaatttc cagcccggcg ccaccgcact
     1381 cattaaaccc gtgtgttgct ctatgggaat atccaacccc aatagtaatt ccccggcgcg
     1441 ggcttcagcg gtgtaaccgt ccatttcggc aaaggccacc tctaattccg ccaccttcat
     1501 gccgtcttct tcgctcattt ccggcaagct gtaaatgcga tcgcgctcgg cttttaccgc
     1561 ccacaattcg gtgtgcccca taatcacggc gtctaatacc gtaaattcct cgtaagcaaa
     1621 ctgatcctgc tttaatttcc ccaaacgcac attgggctcc agcattacct ggccgccggt
     1681 gggcaccaaa tcgccaccca aaattttcat aaaggtggat ttgccgcagc cattagcgcc
     1741 aatcaagccg tagcgattgg agttattaaa tttgacggag acgttttcaa acaggggttt
     1801 agcaccaaat tgcatggtga tattggcggt ggaaatcacg gtaggtacct aaaaatgcgg
     1861 gctattaaaa ttacagatca ataaaaaaca tcagcccaaa aaagtaaggc gcgatggcag
     1921 acaacatcgc gccttgtaga ggctgaacat ctaatggctt aggaagcggc ttccatttgg
     1981 gtttgcaaat aattttgcag gccgacttga ttgattaaac tcaattgagt ttccaaccaa
     2041 tccacgtgtt cttcttcaga ttctaaaatg ctttgcaaaa tatcgcgggt gccgtaatcg
     2101 cgcacggatt cacagtaggc aatggcttct tttaaatcgg gaatggcttt gttttcaagc
     2161 ttaagatcgc aattgagcat ctcttcggtg gtctcgccca ccagcaactt gccgaggtcc
     2221 tgcaaattgg ggataccatc taaaaataaa atgcggtcaa ccaacacgtc tgcgtgtttc
     2281 atctcgtcaa tagactcgtg atactcgtgt ttcgccaggg ctttcaggcc ccagttttta
     2341 tacattttgg cgtgcagaaa atattggtta atggcaatca gttcattgcc gagaataata
     2401 ttgaggtgag cgatgacttt agggtcgcct ttcatagaat gtcctccaca tggcgggtgc
     2461 cctaggcggg ctggggcgtg aaggcgggaa gtgtggaggg ggcaggtagc agtgtcaata
     2521 aatgattgca gggcttagcc ctgcttaaga tagcgaccaa ggggccggtg tgtttaggcg
     2581 acctgataat aggtcgagct ttggcgctca tccagggctt gcaacaacag ctctttggtc
     2641 atgcaaccgc atttgccgca ctgggccata acacccaact gctggcgcaa agcccgcaaa
     2701 gtatccgccc catcctcaat agactggcga atttggcgat ccgtaacccc ttggcaaaga
     2761 cacacgtaca taagctcacc tcaatgacta tgaagcaaga ctattctaaa tgataaccat
     2821 tgtcaattga gttcttattt ggctttgagc cagccaggca atttttgggt gttatgagat
     2881 gacactcaca gaccggccgt gaggacaaaa taggtacacg cccaggcagc caataacttt
     2941 gggcacacaa ctaaaaacgg cagtgccccg cccccaatga catttagttg taactattca
     3001 acactcttta ttcaccccta ctgcgggggc ggcaaattca ggaggattta gccatccggc
     3061 ataatcgatt cgcggtggtg cagtggatta cgggatcaca gcactaaaac actcacgtta
     3121 tagctccctg tcagcgcagg aattgcctcc gactacataa aaagatgctg aatagttaga
     3181 tttagttagg gatttactaa ttagataaca acctagggcg caccttgtgg gtgcctgctg
     3241 ccgcatagat cctgaccctt agccgaagta cactctgggc aaccacaagg attgcccctg
     3301 cacaggttta actgtatgac agtgccccca ccccctatct caataaaggg tttattacgg
     3361 acaacccaag caccttcatt gactggaatt tatttgccgc cccccattat ccgcttgtaa
     3421 cgattagtgc caaaaaaggt ttcggccgtc acccaattgc cttcaaaggt taaattaaaa
     3481 aatactcctt ggcggtaaaa ctggttttct gccagagcaa ataaattaat gacctcatca
     3541 cccagcacaa cttgcaggtt gttctcaccg acttgtaatt tcaaaccatc tcccttgccc
     3601 ccaaccggcg cgtaatcacc tgcaaacttc tgcaagtttt gcttagtaat agttaagttg
     3661 gggcgggcaa ataaaaactg taagcctgca gcatagcctg gaatattcat acccgaatgg
     3721 ccaaacccct caagaaaatc catcttatag tgcaagcctt taatatccgc ttttttaagc
     3781 gcctcgtaat attcctcgct gccacgcacg gctgggtcta acttcccggc gcctgcataa
     3841 aaccggttac cttttaaagg ctgcgccttg gcaagagcat ttatttgcgc ctgcaaactc
     3901 tggtcggcgc cgccatagga gctaccaata gcgagtgcgc cgtcaaacac atccggccgc
     3961 tccattaagg cataggtaac aaacaaaccg ccccaagagc cgcccaccaa aaactcctgg
     4021 ccagtggtgc ggtagcgatt attcaccgcc gggagaattt cgctggcaat agcctgcaca
     4081 aaacccttag cctggccgct attggggatt tggggtagag caactggaga taaatcccgc
     4141 gcccgcaacg cttgcacctt atcgtctgtt atattccaat caatgccgat aaaaatagtc
     4201 tccggcattt ggccgtcgaa atataaacaa ttataagcgc tagtcagcag ggaaaaatcc
     4261 cactggccgt cgattttaat taccacggga tagcgtttat tcaggttggt tgcatagctg
     4321 gcgagggtgc gcaccctaat gcgataatcc tgcccaacaa gtgcagattt cactataaat
     4381 gcttcatagc catccacaga taaaaccggc gatacatccg cactcttggg tg
//
LOCUS       DAOQGS010000343         4425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10538_length_4425_cov_4.179863, whole genome shotgun sequence.
ACCESSION   DAOQGS010000343 DAOQGS010000000
VERSION     DAOQGS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_4425_cov_4.179863"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3473
                     /locus_tag="PKZ52_12555"
     CDS             <1..3473
                     /locus_tag="PKZ52_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60647.1"
                     /translation="RTKTGEERWFDTVIAPFHGQAGQVEKCVALSIDITSRKAVEQEH
                     QRLGQLLRDVLEAASEVAIIATDTHGAITLFNTGAERMLGYSAEEMLGELPFARLHLA
                     DELSSYAASVDNPEDLSGFALLSHQALELGPQTQQWTYVRKDASQLKVSLTLSAMRGQ
                     DGVLVGYLIVAADITESLKQQQALISAKDQLMAAAEVAELGVWEYLPAQQRLHWNERM
                     CEIFGQPLEAPPAGMEDWFSHVHQDERASITAGFSSALASTGEFAGVYRFITPEGSQR
                     YVQFGAYFERDLAGAVFKVTGISRDITGQQELENRLRQAKEQADAANLAKSQFLANMS
                     HEIRTPMNAVLGMLQLVAQTQLGLRQQDYVNKAQTAAKSLLGLLNDILDFSKIDAGKL
                     VIDPTSCSLDDICAEVAVVMAGNQGDKPVEVVFDLAPDLPQRVLIDKLRIQQVLINLI
                     GNALKFTAQGQVWVQLSAEASKADPGRCTVRFCIRDTGIGIREDQQKRIFDGFVQAEA
                     STTRRYGGTGLGLVITKNLIELMGGQLQLASELGQGSEFSFALHLPLGDTPSLAAKAR
                     PAAAGSVLLVHETPLAIEVLERDLTRLGFQVTSAHSAADALSLTEGRARAGEAFSLAL
                     VDWRLSTSNGLSLARQLHDINPEPLGVILLLTQREKEQLAQHQGHDAQFLWQLSKPIV
                     RPALEQVLRLVLAGDQAAPDQAAAPQILAGVSLLVVEDNALNRQVAAELLGGVGAKVS
                     LAVDGRDGVNKATARGAAYDLILMDLQMPVMDGLEATRQLRKQGLNLPILAMTANASA
                     EDKDECLAAGMDGHLSKPIDLNLVVAAIRQALGNTGQVAVAAEAELAAAAGIEPAPTL
                     EQETDQASHLVDADLDESAEALSADADAEFVEGAAEVAGPVPDAHLEQEQEQEQEQEL
                     PVAQALTSGIETQTEARAADEELDLGDLEESLPVVDFFDLLGDELDNEEEPQRAPEDR
                     PEAEAQPAEAQDDLARDTRDAAAPEAAPLFQPPPESVPEGPPGASPQSPAQPQAPVEP
                     SFRETEPPAGDWVDGLDDILRRFGGKRALYQKMLANLGPETERLIAELNSAALAQDRE
                     RASTAAHSIKGTAATLGAKALSQRAAWLEKRIKTSDGPLEEILTPEAFEALAEALAGA
                     LTALDQALAA"
     gene            complement(3752..4375)
                     /gene="thiE"
                     /locus_tag="PKZ52_12560"
     CDS             complement(3752..4375)
                     /gene="thiE"
                     /locus_tag="PKZ52_12560"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNG60648.1"
                     /translation="MLSAPSLYAITDPFLLPGERLFTGVAEALAGGCGWIQYRNKTAA
                     PQAVRLAEAARLNELCQRHGGQLIINDDVALAQAVGAAGVHLGREDGSIAAARALLGP
                     DAIIGATCHASLEFAQQALSEGASYIAFGRFFPSATKPQAKPAPLSLIAAARRLSAPV
                     VVIGGINPQNLPSLKHAGASCFALCEAVFGGADVLARCRQLLALAQA"
BASE COUNT          929 a         1364 c         1384 g          748 t
ORIGIN      
        1 accgcactaa aaccggcgag gagcgctggt ttgatacggt gattgcgccc ttccacggcc
       61 aggcgggcca ggtggaaaaa tgtgtcgccc tcagcattga tatcacctcc cgcaaggccg
      121 tggagcagga gcaccagcgc ctgggccaac tgctgcggga tgtgctggag gcggcctcgg
      181 aagtggcgat tatcgccacc gatacccacg gcgccatcac cctgtttaac accggggcgg
      241 agcgcatgtt gggctacagc gccgaggaga tgctggggga gctgcccttt gcgcggctgc
      301 acctggcgga cgaacttagc agttacgcgg ccagtgtgga taacccggag gacttaagcg
      361 gctttgccct gctgagccac caggctttag agctaggccc gcaaacccag cagtggactt
      421 acgtgcgcaa ggacgccagc cagttaaaag tctccctcac cttaagcgcc atgcgcggcc
      481 aggacggtgt actggtgggc tatttgattg tggcggcgga catcaccgag agcctcaagc
      541 agcagcaggc gctgatctcc gccaaggacc agctgatggc cgctgcggaa gtggcggagc
      601 tgggggtgtg ggaatacctg cccgctcagc agcggctgca ctggaacgag cgcatgtgcg
      661 agatttttgg ccagccttta gaggcgccgc ccgccggcat ggaggactgg tttagccatg
      721 tgcaccagga tgagcgggcc agtattacgg cggggtttag tagcgccttg gcgagcacag
      781 gcgaatttgc cggggtgtac cggttcatca cccccgaggg cagccagcgc tacgtgcagt
      841 ttggcgccta ctttgagcgc gacctggccg gggcggtgtt taaggtcacc ggcatcagcc
      901 gcgatatcac cggccagcaa gaactggaaa accgcctgcg ccaagccaag gaacaggccg
      961 atgcggccaa cctggctaag tcccagtttt tggccaatat gagccacgaa atccgcacgc
     1021 cgatgaacgc tgtgctgggc atgctgcaac tggtggcgca aacccagctg ggcctgcgcc
     1081 agcaggatta cgtcaacaaa gcccaaacgg cggccaagtc cctgctgggg ctgctcaacg
     1141 atattctgga tttctccaaa atcgacgccg gtaaactggt gatcgatccg accagttgtt
     1201 ccctcgacga tatctgcgcc gaggtggcgg tggtgatggc gggcaaccag ggggataaac
     1261 cggtggaggt ggtcttcgac ctggcgccgg acttacccca gcgggtgttg atcgacaagc
     1321 tgcgcatcca gcaagtgctg atcaacctga tcggcaatgc tctgaagttc accgcccagg
     1381 gccaggtctg ggtgcagctg agcgctgagg cgagcaaagc tgaccccggc cgctgcacgg
     1441 tgcgcttttg tattcgcgac actggtatcg gcatccgcga ggaccagcaa aaacgtatct
     1501 tcgacggctt tgtgcaggcg gaggcctcca ccacgcgccg ctatggcggc acggggctgg
     1561 gcctggtgat caccaaaaac ctgattgaac tgatgggcgg gcagctgcag ctggccagtg
     1621 agctggggca gggcagtgaa tttagttttg ccctccacct gcctctaggg gacaccccaa
     1681 gccttgccgc taaggcccgc ccggcggcgg caggctccgt cctgctggtg cacgaaacgc
     1741 ccctagccat agaggtactg gagcgcgatc tcacccgctt aggctttcaa gttaccagtg
     1801 cccacagcgc cgccgatgcc ctgagcctca ccgaagggcg cgcccgtgcc ggggaggcct
     1861 ttagcctggc cctggtggac tggcgcctga gcacttccaa tggcttaagc ctcgcccgcc
     1921 aactacacga catcaaccct gagccactgg gggtgattct gctgctcacc cagcgggaga
     1981 aagaacagct ggcccagcat cagggccacg acgcccagtt cctctggcag ctgagcaaac
     2041 ccatagtgcg gcccgccctt gagcaggtgt tacgcctggt attggccggg gatcaagccg
     2101 cgccggacca agccgcagcc ccgcaaattc tcgccggggt gtcccttttg gtggtggaag
     2161 ataacgccct caaccgccag gtggcggcgg agctgcttgg cggcgtgggt gctaaggtga
     2221 gcctggcggt ggacggccgc gacggcgtca acaaggctac ggccaggggt gccgcctacg
     2281 atttaattct catggacctg caaatgccgg taatggatgg cctggaggcc acccggcaac
     2341 tgcgcaaaca gggcctgaac ctgcccatcc tcgccatgac cgccaacgcc tcggcggagg
     2401 acaaagatga gtgcctcgct gccggaatgg atggccactt gagtaaaccc atcgatctaa
     2461 acctggttgt ggccgccatc cgccaggcgc tcggcaacac cggccaggtt gctgttgcag
     2521 ctgaggccga gcttgccgct gcggctggga ttgagccagc gcccacactt gagcaagaga
     2581 ctgatcaggc ctcgcacctg gttgatgcag acttggatga gtcggcagaa gccttatcgg
     2641 ctgatgccga tgcagagttt gttgaaggtg cggcagaagt agccgggcca gtgccggatg
     2701 ctcatttgga gcaagagcaa gagcaagagc aagagcaaga gctgcccgtt gcacaggcct
     2761 tgacgagcgg cattgaaacc caaaccgaag cgcgggcagc ggacgaggaa ttggaccttg
     2821 gcgatctgga ggagagcctg ccggtagtgg acttctttga tttgctaggg gatgaactcg
     2881 ataacgagga ggagccacag cgagccccag aggacaggcc cgaggctgaa gctcagcctg
     2941 ctgaggccca ggatgatctg gccagagata cccgcgatgc agcagcacct gaggcagcgc
     3001 ctttattcca gccgccgcca gaatccgtac ccgaggggcc gcctggcgcg tcgcctcaat
     3061 ccccggccca accgcaagct ccagttgaac cctccttccg cgagaccgag ccgccagccg
     3121 gcgactgggt agacggcctg gacgacatac tgcgccgctt cggcggcaaa cgcgccctct
     3181 accaaaagat gctcgccaac ctggggccgg agacggagcg tttaatcgct gaacttaaca
     3241 gcgcagcctt ggcgcaggac cgcgaacggg ccagcaccgc cgcccattcc atcaaaggca
     3301 ccgccgccac cctaggcgcc aaagccctga gccagcgcgc cgcctggctg gaaaaacgca
     3361 tcaaaaccag cgatggcccc ctagaggaaa tcctcacccc cgaggcattc gaggcactgg
     3421 cagaagccct cgccggagcg cttacggcgc tggatcaagc cctggcggct taatgaagca
     3481 cttaatcaat tccactgtag aaaatcgaac ccactaagtt aaggagaagc ccattctggc
     3541 gtaggttgga agagcgcagc gacttccgac atcccgccga gccattgcaa tgcctagctt
     3601 ttatgctggc ggatggatgt cggaagccgc tcggcaattg ctcctgcatt gctctaccac
     3661 atccatgtga ctatgcgcgc tgccaaccta cgagtaaaaa ctcacctcat aacgcctaac
     3721 ctaatgacac ttcctctgga gctgtgccgc cttaggcctg cgccaacgcc agcaattgtc
     3781 ggcagcgcgc cagcacatcc gccccgccaa acaccgcttc acacagggca aaacagctcg
     3841 cccccgcgtg ttttaagctc ggcaaattct gcgggttgat gccgccgatc accactacag
     3901 gggcacttag gcgccgggct gcggcaatca aacttaaggg ggcgggtttg gcctggggtt
     3961 tggtcgcaga gggaaaaaag cgcccgaagg caatataact ggcgccctca ctgagggcct
     4021 gctgggcaaa ctcaaggctt gcatggcagg tggcgccgat gatggcatct ggccccaaga
     4081 gggcgcgggc ggcggcgatg gagccgtcct cgcggcccaa atgtactcca gcggcgccca
     4141 ccgcctgggc cagcgccaca tcatcattaa taatcagctg gccgccatgg cgctgacaaa
     4201 gctcattgag gcgcgcggct tcagccaggc gcaccgcctg tggggcggcg gttttattgc
     4261 ggtactgaat ccagccacag ccgcctgcta aagcctcggc caccccagta aacagccgct
     4321 cgccgggcag taaaaagggg tctgtaatgg cgtagaggct gggggcgctg agcattaaaa
     4381 cggcttcacg atcaccagaa tggcgatgag aattaaaaac accac
//
LOCUS       DAOQGS010000344         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10559_length_4419_cov_5.690880, whole genome shotgun sequence.
ACCESSION   DAOQGS010000344 DAOQGS010000000
VERSION     DAOQGS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10559_length_4419_cov_5.690880"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PKZ52_12565"
     CDS             <1..1453
                     /locus_tag="PKZ52_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HNG60649.1"
                     /translation="WQVVDAEGSARTLNFEVRKALYDDETCVQLVVKPGVARGEVQVA
                     AQVQQIRNQDLVTGLFNRHHLVEQLEQVLARTLTDHSTSALVSIEVEDFLGIVQTRLG
                     VAAADIALSIIAAHLKSLCQNGEILCRYSDDCFMLLMPSISADAALGRAEAIGRNLRE
                     HIVTVDDVTLMFGYALGIGLINDTTVSAETPIAHAIKALELLHKERAQNKNLSARIYE
                     PEVAKANQEREHSEKQVAQMVQKALDQGRFKLLFQPILSLRGSDKEHYEVLLRMREVD
                     GEDLTPAEFFDVAEKMGAMIRIDRWVILESIKLLAEHRGKGHPTRMIIHLSESSIKDK
                     SLVPWLAVAFKAAKLAADGLIFQLNEVDVNNNLNHAKELTFALQALGCDVCINHFGCV
                     LHPFAVLSHLAANFVKVDGSFTQELQSGHSEPKALADLVGQLHQLEKITIVPFVENAS
                     VLSKLWQSGVHFIQGYYLQGPAESMDFNFDTEN"
     gene            1995..3551
                     /locus_tag="PKZ52_12570"
     CDS             1995..3551
                     /locus_tag="PKZ52_12570"
                     /inference="COORDINATES: protein motif:HMM:NF028300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM35 domain-containing protein"
                     /protein_id="HNG60650.1"
                     /translation="MRLAILGAVSTALTACGGGILPGVGGKSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSGGQAYGLWIQESTLGFCDFKGVVDTVYSGFTGTGYADSENALNAS
                     VSYSVVAANAGTYNTAVRYANGASSARGYDIFVDGTKVKSGLGTSTTSWTNWSEELIE
                     LNLPAGAHTITLRATTASGLPNIDRVGVKETGVSAAACAVVGSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGAVGN
                     AATGATLYGNSTGKCATCHYNFGEGDIAGTDGGIPPIEPLKTQYTTYAGALLPVALNL
                     NDYIKAYMPSKNKLTVQQAADVTAYATTLPSAIRGKALFESATQGCATCHGADGSGGL
                     SLIPLSTAAMIDPNGSGYLTIEGIAAKIVDSMPQTKTTYKNKTLNTKYGSCDQACAND
                     IAKYIWTTFP"
     gene            3671..>4419
                     /locus_tag="PKZ52_12575"
     CDS             3671..>4419
                     /locus_tag="PKZ52_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60651.1"
                     /translation="MNKLNLIIGGIFALSLSSAHGEVLLYEQDFEHPVNFVNDGGDVN
                     IFRTVNNLYANQPAGFEFAQTYTVETLLVGGSSAWTVAGNPGGGFKDPQGIAGKHVIS
                     MLSDRQDDWLALSFDVGAYKFLNFDLDISSIDLNNFGGPFYDGKAPKFQLSLYDNPSG
                     AVGLGSGSFLSRAEASGTLADNRWTFNWSHAAVGLSTAGNTNGHVTLRVDLLEGGYGA
                     LDNFRIAASDIPAVPEPANWLMLVAGLGLTAR"
BASE COUNT         1059 a         1205 c         1054 g         1101 t
ORIGIN      
        1 ctggcaggtg gtagatgccg aaggcagtgc tcgaaccctc aattttgaag tgcgcaaagc
       61 cctctacgac gatgaaacct gcgtgcagct ggtggtgaaa cccggcgtcg cacggggtga
      121 agtgcaggtg gcggcgcagg tgcaacagat ccgcaatcag gacctagtca ccggcttgtt
      181 taaccgccac catttagtgg aacaactcga acaagtgctc gcccgcaccc tcacggatca
      241 ttccacctcg gccctggtca gcatcgaagt ggaagacttc ctcggcatag tgcaaacccg
      301 cctgggtgta gccgctgccg acatagccct cagcattatt gccgcccact taaaaagcct
      361 ctgccagaac ggcgaaatcc tctgccgcta cagcgacgat tgttttatgt tgctgatgcc
      421 gagcatttcc gccgacgccg ccctaggccg cgccgaagcc ataggccgca atctgcgcga
      481 gcacatagtc accgtagacg atgtcaccct tatgttcggt tatgccctgg gcataggatt
      541 gatcaacgac accaccgtct cggcggaaac ccccatagcc cacgccatta aagcccttga
      601 gctgttacac aaagagcggg cgcagaataa aaatctgtcg gcgcgcatct atgaacccga
      661 ggtggccaag gccaaccaag agcgcgaaca cagcgaaaaa caagtggccc agatggtgca
      721 aaaagccctg gatcagggcc gctttaaact gttattccaa ccgattttga gcctgcgcgg
      781 ctcggataaa gagcattacg aagtgctgct gcgcatgcgc gaggtggacg gcgaggacct
      841 aactcccgca gagttctttg acgtggccga aaaaatgggc gccatgatcc gcatcgatcg
      901 ctgggttatt ttggaatcca ttaaattgct ggccgaacac cgcggcaaag gccatcccac
      961 gcgcatgatc attcacctaa gtgaatcctc gattaaggat aagtccttgg tgccctggtt
     1021 ggcagtggca tttaaggcgg caaaactcgc cgccgacggc ttaatttttc aattaaatga
     1081 ggtagacgtt aataacaacc tcaaccacgc caaagagcta acctttgctc tgcaagccct
     1141 cggttgcgat gtgtgtatta accacttcgg ttgcgtgctg caccccttcg ccgtgttaag
     1201 ccatttggcg gccaattttg ttaaggtgga cggctccttt acccaagaat tacaaagcgg
     1261 ccacagcgag ccaaaagccc ttgctgatct ggtggggcaa ttgcatcaat tggaaaaaat
     1321 caccattgtg ccctttgtgg aaaacgccag cgtcttgtcc aaactctggc aatccggcgt
     1381 gcattttatt cagggatatt atttacaggg gcccgccgaa agtatggatt ttaatttcga
     1441 tacggaaaat taatacccct attgtgcacc cactaacgaa gctgtaattg ttttttaccc
     1501 cgcggtttaa tagccccaaa gcgttttttg catttccctt cgcatcatta atcagactaa
     1561 agtttgaaat taaaatctgc ataccccgcc ttgggctgac ttctttgctt cgcaaaatcc
     1621 aacttcccat ggaaattcgc gatgaaaatc aaaataatca gatatttttt cgtgatctga
     1681 gtcttgattt gctgtgacca aaaacacatt cccagtattt ctttagggct ataccgccga
     1741 ttgtgacctg cggcgcggaa ggccctgcgc cgctgattag aatggtacgg gtgtgagtgt
     1801 ctctctaaac aagcctgcga gcctccagca attccataac tgaacccagc caccgattaa
     1861 taaagtatta ataagtaccc ccgagctgcc ggaagctctc gcggtgaaca tgggttccta
     1921 agaccacaac tatatcctcc tgggagttta ttgcgatgtc tgttacgaga tttagcaacg
     1981 ttaaaacgct agcgatgcgt ttagctattt taggtgctgt aagcacagcg ctgaccgcgt
     2041 gcggcggcgg cattttaccg ggcgtcggcg gcaagagttc atcctctagt tccagttcta
     2101 gctcaagctc tagttcatcc tcttcctcta gctcatcctc ctcaagcagc agctcaagct
     2161 ccagcggtgg ccaagcttat ggcctctgga ttcaagaaag caccctcggc ttctgcgact
     2221 ttaaaggcgt agttgatact gtttattccg gctttaccgg cactggctat gcggactctg
     2281 aaaatgccct aaatgccagc gtaagctata gcgtagttgc ggccaacgca ggcacctaca
     2341 acaccgccgt gcgctatgcc aacggcgcct cctccgctcg cggttatgac atttttgttg
     2401 acggcaccaa agtaaaatca ggccttggca cctccaccac tagctggacc aactggagcg
     2461 aagaactgat cgagctgaac ctgcccgctg gcgctcacac catcaccctg cgcgccacca
     2521 ctgcatcggg tcttcctaac atcgaccgag taggcgtgaa agaaaccggc gttagcgccg
     2581 ctgcctgtgc cgtcgtgggt tcatcgtcca gctcttctag cagctcaagc tctagcagtt
     2641 cttcttcaag ttcttctagc agctcaagct ccagcagttc ttcttcaagt tcttccagca
     2701 gctcaagctc tagcagttct tcttcaagtt cttccagcag ctcaagctct agcagttctt
     2761 cttcaagttc ttctagcagc tcaagctcta gcagttcttc ttcaagttct tccagcagct
     2821 caagctctag cagttcttct tcaagttctt ctagcagctc aagctctagc agttcttctt
     2881 caagttcttc cagcagctca agctctagca gctcttcttc aagttcttct agcagttcaa
     2941 gctccagcag ttcttcttca agttcttcta gcagctcaag tggtgctgta ggtaacgctg
     3001 caactggtgc aactctgtac ggcaactcaa caggcaaatg tgcaacctgt cactacaact
     3061 ttggcgaagg cgatattgct ggtactgacg ggggaatccc accaattgaa ccgctaaaaa
     3121 ctcagtacac cacctatgcc ggtgcattgt taccagtggc gcttaacttg aacgactaca
     3181 tcaaagccta tatgccgtct aagaacaaat tgactgttca gcaagcggcg gacgttacgg
     3241 cctatgcaac taccttaccc tcagcaattc gcggtaaggc cctgttcgaa tctgcaaccc
     3301 agggctgtgc aacctgccat ggcgccgatg gttcgggtgg cctttctcta atccccttga
     3361 gtacagcggc aatgatcgat ccaaatggtt ctggatacct gactatcgag ggtattgcag
     3421 cgaagattgt agactccatg ccacaaacaa aaacgaccta caaaaacaaa acgctcaata
     3481 ccaaatatgg cagctgtgac caagcctgtg ctaacgacat agccaaatat atctggacca
     3541 ccttcccata atgggttagt gatttagatt tgaaaaaggc agcttcggct gcctttttta
     3601 tgccccaacg tttgccaggc cattaatctg ctctatgctg ggtacccctt ttaaaattcc
     3661 ggagcatgtt atgaataaat tgaacttaat tatcggcggt atttttgccc tttccctcag
     3721 cagtgcacat ggggaggttt tattatatga acaggatttt gagcacccgg ttaattttgt
     3781 aaacgacggc ggcgatgtga atatctttcg caccgttaat aatctttatg ccaatcaacc
     3841 agctggattt gaatttgccc aaacctacac ggttgaaact ctgctagtgg gcggcagctc
     3901 cgcctggacg gtggcaggca atcccggcgg agggtttaaa gacccccaag gcattgccgg
     3961 taagcacgtg atttccatgt tatccgatcg ccaggacgat tggttggcgc tgtcttttga
     4021 tgtgggcgcc tataaatttc tcaattttga tctcgatatt tcatccattg atttaaataa
     4081 ttttggcggg ccattttatg acggcaaggc gcctaaattt cagctctcct tatacgacaa
     4141 tccaagcggt gccgtaggtt tggggagcgg cagttttctc tcccgcgccg aggccagcgg
     4201 cactttggca gataatcgct ggacatttaa ttggagccac gctgccgtcg ggctgagtac
     4261 cgctggcaat accaacggcc atgtcacgct gcgggtggat ttactcgaag gcggttatgg
     4321 cgccctggac aattttagaa tcgccgcctc ggatattccg gcagtgcccg aaccggccaa
     4381 ttggctgatg ttggtggcgg ggttagggct gacagcccg
//
LOCUS       DAOQGS010000345         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10618_length_4403_cov_4.255980, whole genome shotgun sequence.
ACCESSION   DAOQGS010000345 DAOQGS010000000
VERSION     DAOQGS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_4403_cov_4.255980"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKZ52_12580"
     CDS             <1..408
                     /locus_tag="PKZ52_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNG60652.1"
                     /translation="LGFALVGASLLAGACNLLLRGELAAEALGVPVVPVRWALFLLAC
                     GCTALAVSIGGNLGFIGLVAPHSLRLLGVWDQRILLPACCVLGALLLVLADTAARLLL
                     APVQIPVGVLTAVLGVPLLLYLLRQPAYGGRDA"
     gene            401..1183
                     /locus_tag="PKZ52_12585"
     CDS             401..1183
                     /locus_tag="PKZ52_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG60653.1"
                     /translation="MLRCSNLTLQRGGKRILHRLDWQVAAGEVWGVLGANGAGKSSLL
                     LCLAGLLPAAAGEVHLLGRPLAAHRPRERACSLGFLAQDQTADLPIRLRDWLLLSRYP
                     KQGLWGRQRLCDLAKVAQVLEQTQLTPLASRYTCELSGGERQRMALAGLLVQAAPWLV
                     LDEPTNHLDLHQQMSLLPQLIAASCGGGGALVMSLHDVNLAEQFCSHCLLLFEGGEIA
                     AGPVAQVLTAEAISRIYRHPIEVILGPSGRVFVPRRLVESLK"
     gene            1342..1803
                     /locus_tag="PKZ52_12590"
     CDS             1342..1803
                     /locus_tag="PKZ52_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60654.1"
                     /translation="MPCVNRVRKFGQVVLALGGCIAGGALAANDGSVLWALDNQKNQN
                     IKLLMPTFSGSFENRSLVLVFSPVKYTKQKVEFSVAVTAACSRSPVLSKSYSVTTYPA
                     AENGGRFKLALPKHLARCFKVDPKGEISVAMEFRSSPVDELVSLRGKAFIE"
     gene            complement(1793..2944)
                     /gene="queG"
                     /locus_tag="PKZ52_12595"
     CDS             complement(1793..2944)
                     /gene="queG"
                     /locus_tag="PKZ52_12595"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNG60655.1"
                     /translation="MSTSPFYLELTAALRRRAQELGFSAMGVSDTDLHRYIPRARAWI
                     DQGNHLPLEWMAEQQALKENPAQLVPGTLRIISLRMDYLPSRKGHIEVLKDPNKAYIA
                     RYALGRDYHKTLRKRIAQLGDFMRDWVRRHRPDEAIAYRAFVDSAPIMERPLAEKAGL
                     GWIGKNSLIIHPQAGSFFLLGELFTNLPLPLDTLPVANQCGDCTACLSICPTDAFRAP
                     YVLETQRCIAYLTIENKGPIPEEYREAMGNRVFGCDDCQAICPWNKFAGVAQVGDFAP
                     RHNLQQEDLLTLFNWDETTFLHHTQGSAIRRIDYRRFLRNLSIGLGNAPSHPDIIAAL
                     KTRLNDGALFDEETPWLQEHFEWALAQQLNPHRRRKRKIKRPAEQAPIQ"
     gene            3094..>4403
                     /locus_tag="PKZ52_12600"
     CDS             3094..>4403
                     /locus_tag="PKZ52_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNG60656.1"
                     /translation="MPALPQGLYPAAAVKAIDAAAIAAGTPGVVLMKRAARAALNLIL
                     ARYPQAHLWIFCGGGNNGGDGYVLAGLAKSRGLQVSLFWAEDPAKLSREAADAYAFAL
                     QEQVEIQPASALPAQVPEQLLIVDALLGIGLTGEVRPATAALIQKLNQYRAPRLALDI
                     PSGLCADTGRVLGAALQASLSLCFVALKPGLFTGSGPALCGEVLFDDLAIAPEFYPPA
                     PITRVDAQTCAGLLPRRAADAHKGHSGHVLVVGGELGMGGAGLLCSEAALKVGAGLVS
                     LATRAEHVLASQVRRPEIMAKAVNTAADLAGLTAKASVLALGPGLGQEPWGQQLHQSV
                     LSSGKPLVLDADGLNLLARTTAGLSCPSPCIITPHPAEAARLLGITTEQVQADRLAAA
                     ANLAAKTQAVVVLKGAGTVIASGNQRFIAKVGNPVLATAGSGDVL"
BASE COUNT          703 a         1250 c         1495 g          955 t
ORIGIN      
        1 ctggggtttg cgctggtggg tgcctctttg ttggcgggtg cgtgcaattt attgctgcgc
       61 ggggagctgg cggctgaggc cctgggcgtg ccggtggtgc cagtgcgctg ggcgctcttt
      121 ttgttggcct gcggctgcac ggccctggcg gtgagcatcg gcggcaactt ggggtttatt
      181 ggcctggtgg cgccccacag tttgcggctg ctcggagtgt gggatcagcg cattttattg
      241 cccgcctgct gtgtgttggg ggcgctgctg ttggtgctgg cggacaccgc cgcgcgcctg
      301 ctcctggcgc ccgtgcaaat cccggtgggg gtgttaaccg ccgtgttggg tgtgccccta
      361 ttgttgtacc tgctgcgcca accggcctat gggggccgcg atgcttaggt gttctaacct
      421 aaccctgcag cgcggcggta agcggattct gcaccgcttg gattggcagg tggcggcggg
      481 ggaggtatgg ggtgtgttgg gggccaatgg ggcgggcaag agcagtttgc tgctgtgttt
      541 ggcgggcctg ttgcccgcag cggccggtga ggtgcatctg ctggggcgcc ctttggcggc
      601 ccatcgcccc cgcgagcgcg cctgcagttt ggggtttctc gcccaggatc aaaccgccga
      661 cctgcccatt cgcctgcgcg attggctgtt gctcagccgt taccctaagc agggcctgtg
      721 gggccgccag cgcctgtgcg atctggccaa agtggcgcag gtcctagagc agacgcagtt
      781 aaccccttta gccagccgct atacctgtga gctgagcggc ggtgagcgcc agcgtatggc
      841 cttggcgggc ctgctggtgc aggcggcgcc ctggctggtg ttggatgagc ccaccaatca
      901 cttggacctg caccagcaga tgagcctgct gccgcagttg attgcggcta gctgcggggg
      961 tggcggtgcc ttggtgatga gcctgcacga tgtcaattta gccgagcagt tttgcagcca
     1021 ctgtctgctg ttgtttgagg gtggggagat tgcggcgggg ccggtggcgc aggtgttgac
     1081 ggcagaagcg attagccgca tctaccgcca cccgattgag gtgatcctgg ggccgagcgg
     1141 gcgagtgttt gtgccccggc gcttagttga gtcgcttaag taatcggagc gcctgtgcgc
     1201 ttctgcacag tgttcaggat tttttagcgg aggttgagtg gggcagggta atcttgcgcc
     1261 cgcgttatag tgaacgccct gggcttctgt gcccctgggg cgattggttg gtgctgcgtg
     1321 cagcctagat gagttgagct tatgccctgt gtaaatcgcg ttagaaaatt cggccaagtg
     1381 gtgttggctt taggaggttg tatcgcaggc ggcgccctgg cggccaatga cggcagtgtg
     1441 ctgtgggcgc tggataatca gaagaatcaa aacatcaaac tattgatgcc cacttttagc
     1501 ggcagctttg aaaaccgcag cttggtgctg gtatttagcc cggtgaaata caccaaacaa
     1561 aaggtggagt tctctgtggc ggtgaccgcc gcttgctccc gcagcccagt gctgtccaaa
     1621 agttatagtg tcaccaccta cccggccgct gaaaacggcg gccgctttaa gctcgctctg
     1681 cctaagcatt tggcccgctg ttttaaggtg gacccgaagg gggagattag tgtggcgatg
     1741 gaatttcgct caagcccggt ggatgagctg gtgtccctgc ggggcaaggc gtttattgaa
     1801 taggggcttg ctcggccggg cgtttgattt tgcgtttgcg ccgccgatgg gggtttagct
     1861 gctgggctag ggcccactca aaatgctcct gcagccaggg ggtttcttca tcaaacaagg
     1921 cgccatcgtt caggcgtgtc ttaagggcgg cgataatatc cgggtggctt ggcgcattgc
     1981 caaggccgat ggataggttg cgcaggaagc ggcggtagtc gatgcgccgg atggcactgc
     2041 cctgggtgtg gtgcagaaag gttgtttcat cccagttaaa tagggtcaat aaatcctcct
     2101 gctgcagatt gtggcggggg gcaaagtccc ccacttgggc caccccggca aacttattcc
     2161 aggggcagat ggcctggcag tcgtcgcagc cgaatacccg gttgcccatg gcctcacggt
     2221 attcctctgg aatcgggccc ttgttttcga tggttaaata cgcgatgcag cgctgggtct
     2281 ccagcacata gggcgcccgg aaggcatcgg tggggcagat gcttaaacag gcggtgcagt
     2341 cgccgcactg gttggccaca ggcagagtat ccaaaggcag gggcaggttg gtgaacagct
     2401 cccccaagag aaaaaaactg ccggcctggg ggtgaataat caggctgttt ttgccgatcc
     2461 agcctaagcc tgctttttcc gccagggggc gctccattat cggtgcgcta tccacaaacg
     2521 cgcggtaggc gatggcttca tccggccggt ggcggcgcac ccaatcgcgc ataaagtccc
     2581 ccagctgcgc aatgcgtttg cgcagggttt tgtggtagtc ccggcccagt gcgtagcggg
     2641 cgatgtaggc tttgttggga tctttgagca cctcgatatg ccccttgcgg ctgggcaaat
     2701 agtccatccg cagggagata atgcgcaggg tgccgggcac cagttgggcg gggttttctt
     2761 ttagcgcttg ttgctcggcc atccactcta ggggcaggtg attgccctgg tcgatccagg
     2821 ccctggccct tgggatgtag cggtgcaggt ctgtgtcgct cacgcccatg gcgctaaacc
     2881 ccagctcctg cgcgcggcgg cgcagggcgg cggtcagctc gaggtaaaag gggcttgtgc
     2941 tcataggggc ttggggtggc gcgcaggggc ggctattgtg cctgaaaaga cctcagctgg
     3001 cgctgcggga atctgtgcat tttgcggcct taagcgcttg ggggcggcac tgggcctgcg
     3061 tatactagcg ggcctctttt ttggtgagat tctatgccag ctctgcccca gggcctctat
     3121 ccggccgccg ctgtaaaagc cattgacgcc gctgccattg cggcgggcac ccctggcgtg
     3181 gtgctgatga aacgcgccgc ccgcgccgcc ctcaatctga tcctcgctcg ctacccccaa
     3241 gcgcacctgt ggatcttctg cggcggtggc aacaatggcg gcgacggtta tgtgctggcg
     3301 ggcctggcca aatcccgcgg cctccaggta agcctattct gggcggagga cccggccaag
     3361 ctctcccgtg aagccgctga cgcctatgct tttgctctgc aagaacaggt agagatacaa
     3421 ccggccagcg ccctgcctgc tcaagtcccc gagcagctat tgattgtgga tgcgctgctg
     3481 ggtatcggct tgacaggtga agtgcgcccg gccaccgccg cgcttattca gaagcttaac
     3541 caataccgcg ccccccgcct ggctttggat attccctccg gcctctgtgc cgataccggc
     3601 cgggtgctgg gcgcggcgct gcaggcgagc ctcagcctgt gttttgtcgc cctcaagcct
     3661 ggcctcttca caggctcagg ccctgccttg tgcggcgagg tgctgtttga tgatcttgcc
     3721 atcgcccctg aattttaccc gcccgcgccc atcacccgcg tggatgcgca aacctgtgca
     3781 ggcctgttgc cgcgccgggc ggcagatgcc cacaagggcc acagcggcca tgtattggtg
     3841 gtgggtggcg agctgggcat ggggggcgcc gggcttttgt gcagcgaggc cgcccttaaa
     3901 gtgggggcgg ggctggtcag ccttgccacc cgggccgaac acgtattggc cagccaagtg
     3961 cgcaggccgg aaattatggc caaagccgtc aacacagcgg cggatttagc aggccttacg
     4021 gctaaagcaa gcgtgctcgc cctaggccct ggcctggggc aggagccttg gggccagcag
     4081 ttgcatcaaa gcgtgctcag cagcggcaaa cccctggtgc ttgatgccga tggcttaaac
     4141 ctgttggctc gcaccaccgc aggcctcagc tgccccagcc cctgcattat taccccccat
     4201 cctgctgagg cggcccgctt gctgggcatt accaccgagc aggtgcaggc cgatcgctta
     4261 gccgccgctg cgaatcttgc cgccaagacc caggcggtgg tggtgttaaa aggggcgggc
     4321 actgtgatcg cctcgggaaa tcagcgattt attgccaagg taggcaaccc ggtgctcgcc
     4381 accgcaggca gcggcgatgt gct
//
LOCUS       DAOQGS010000346         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10642_length_4398_cov_4.437716, whole genome shotgun sequence.
ACCESSION   DAOQGS010000346 DAOQGS010000000
VERSION     DAOQGS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_4398_cov_4.437716"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /gene="ccsA"
                     /locus_tag="PKZ52_12605"
     CDS             <1..183
                     /gene="ccsA"
                     /locus_tag="PKZ52_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNG60657.1"
                     /translation="AQKTVFSLMAWATYALLLWGRHAWGWRGAAAMRWTFTGFAFLLL
                     AYFGSKFVLELILQRA"
     gene            complement(189..755)
                     /locus_tag="PKZ52_12610"
     CDS             complement(189..755)
                     /locus_tag="PKZ52_12610"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNG60658.1"
                     /translation="MAENTDNRRRVAAVLVLERGSPSNPEILLVKRARHLNSHAGEVG
                     LPGGMAEPFDQDLWHTALREAQEEVGLVPDSVLLQRSLRHAFTRQGVEVAPFVAAWQQ
                     EHPLSLCADEIESAFWLPVSFLKQDQRVRTDIFPRQTKEYWAPVYRFAGQIIWGFTAR
                     LLVEYMNLCHGAAIGRSHQAPVARFKPA"
     gene            1000..1326
                     /locus_tag="PKZ52_12615"
     CDS             1000..1326
                     /locus_tag="PKZ52_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HNG60659.1"
                     /translation="MQSEWPAVDELIALAKESPAQLEAFRRHEIERLIASAPESIQRR
                     LRGLQFQIDCKRRLHSSPMGACIELSQMMFDSVSQLNNALQGISANRQHPPKQAASVL
                     PFTKAV"
     gene            complement(1337..1690)
                     /locus_tag="PKZ52_12620"
     CDS             complement(1337..1690)
                     /locus_tag="PKZ52_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HNG60660.1"
                     /translation="MNEQWQEGEDYRVKLCFYVPVDALDAVKAAIFAAGAGALGHYQH
                     CAWQTLGQGQFKPLTGAKPQIGAVGELVSVPEYRVEVLCSGRNAAAVEAALLKAHPYE
                     APAYDFTLLWRPTPQ"
     gene            1695..2522
                     /locus_tag="PKZ52_12625"
     CDS             1695..2522
                     /locus_tag="PKZ52_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNG60661.1"
                     /translation="MRLFVDQLTNVDFSYLCPERGLLGETWLASIHLVGSLDEQGMIC
                     DFGRVKKHLRQWLDDTLDHKLLVPSLAPQLAAEDNNSLSFNYGGGLVLKTCGPATAFC
                     HIPTAQITPASVAAWCIGQLKGDFGPGVSSIELNFRPEQTDGPFYHYSHGLKKHLGNC
                     QRIAHGHRSIIKIWRNGQLCETSMEQVAKAWRDIYIGTRADIVAEHADHWEFAYSASQ
                     GNFRLQIPKAAVYLMDEDTTVENISAHLAAKLKAESPQDQLVVKAYEGLGKGAEVSC"
     gene            2859..3722
                     /locus_tag="PKZ52_12630"
     CDS             2859..3722
                     /locus_tag="PKZ52_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNG60662.1"
                     /translation="MSSSTALLGFNDPFSSWTHLLAAVFFGIATFFLLKKGKGNAVRI
                     AALSLYSFALVFLFSMSGVYHLLAQTGDARAVLQRLDHAAIWVLIAGTFTPIHVILFR
                     GPWRWLILSTVWGIGLLGLIFEAIFFTSFPQWLITSLFLGLGWMGALTGYKFRKAYAG
                     EKLRGLVLGGIFYSIGAVCDFLQWPVIWPSYIGGHDVFHIFVILGAISHWRFIYKWAA
                     HSASNKMIFHVSTFPDGAVVGQAVGDHIRVEASDLHELKARIKTLVKTKFHSSIEPEI
                     HLKYFNEERIQ"
     gene            complement(3827..3914)
                     /locus_tag="PKZ52_12635"
     tRNA            complement(3827..3914)
                     /locus_tag="PKZ52_12635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3878..3880),aa:Ser,seq:gga)
     gene            4104..>4398
                     /locus_tag="PKZ52_12640"
     CDS             4104..>4398
                     /locus_tag="PKZ52_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60663.1"
                     /translation="MESPNFLLILAWEAFGLALVAVAVLLFQNRKMRSLVNKLQARMH
                     ELVGDLKKSRDELNKKPPVREETYLHYINQHLEQTKKHHFSFRGGQDIMLDLDP"
BASE COUNT          991 a         1304 c         1111 g          992 t
ORIGIN      
        1 gcgcaaaaga ctgtattttc ccttatggcc tgggccacct acgcgctgct gctgtggggg
       61 cggcacgcct ggggttggcg cggcgcggcg gccatgcgct ggaccttcac aggcttcgcc
      121 tttttattgc tggcctactt cggcagcaaa tttgttctcg aacttatact gcagcgcgct
      181 taacaatctt aagcgggctt gaagcgcgcc accggcgcct ggtggctgcg gccaatagcg
      241 gcaccgtggc agagattcat gtattccacc aacaagcgag cggtaaaccc ccagataatc
      301 tggccggcaa agcggtacac cggcgcccag tattcttttg tttggcgggg aaaaatatcc
      361 gtgcgcaccc gctggtcctg ctttaaaaaa ctcaccggca accaaaaggc cgactcaatt
      421 tcatcggcac acaggctcaa ggggtgctcc tgctgccaag cggctacaaa cggcgccact
      481 tccaccccct ggcgggtaaa ggcgtgccgc aaggagcgct gcaatagcac cgaatccggt
      541 accaggccca cctcctcctg agcctcccgc aaagcggtat gccacaaatc ctggtcaaac
      601 ggttctgcca tgccgccggg cagccccacc tccccggcat gggaatttaa atgccgcgcg
      661 cgttttacca acaggatttc cgggttgctc gggctgccgc gctctagcac cagcactgcg
      721 gccactcgcc gccggttgtc tgtgttttcg gccatatcat tactcacttg ctcacacact
      781 ctttactcga caagttcaca ttgcgcgcac aattctcaat tgtccacccg gcctgaggcc
      841 gcgcaattcc agcatataaa aatttgatat aggttttgct attaacatag cgaatagaat
      901 cttgatagta tgatttttaa tcaagcaaca gccccccaac acctggttta cataggggga
      961 gctgtctttc cgtaaggctt gcctgcaacg aggtacccca tgcaatccga atggcccgca
     1021 gtggatgaac tgatcgctct tgccaaagag agccccgcac aacttgaggc gtttcgccgc
     1081 catgaaatcg agcgcttgat cgcctcggcg cctgagtcca tccagcgtag gctgcgcggc
     1141 ttgcagtttc aaattgactg caaacgcagg ctgcacagct cacctatggg ggcctgtatt
     1201 gagctctccc agatgatgtt cgactcggtg tctcaactga ataatgctct gcagggcatc
     1261 agtgccaaca gacaacaccc gcccaagcag gcggcctcgg tattaccctt caccaaagcg
     1321 gtataaaaag gcgccattat tggggcgttg ggcgccacag tagggtgaag tcataggcgg
     1381 gcgcttcata gggatgtgcc tttaacaggg ccgcctccac tgcggcggcg tttcgcccgc
     1441 tgcacagcac ttcaaccctg tattccggca cagacaccag ctctcccacc gcgccgattt
     1501 gcggcttggc gcccgtcagt ggcttgaact gcccctgccc caatgtttgc caagcgcagt
     1561 gctggtaatg ccccaaagca ccagccccgg cggcaaaaat ggccgctttg acagcatcaa
     1621 gcgcatctac cggcacataa aagcacagtt tcaccctata atcctccccc tcctgccact
     1681 gctcgttcat agccatgcgc ctttttgttg atcagctcac caatgtcgat ttcagttacc
     1741 tctgccccga gcgcgggctc ctgggcgaga cttggcttgc cagcatacac ctagtgggca
     1801 gcctggatga gcagggcatg atttgcgatt ttggccgagt taaaaaacac ctgcgccagt
     1861 ggctggacga cacgctggac cacaaattat tagtgccaag cctcgcgcca cagctggccg
     1921 cagaggataa caatagcctc agcttcaact atggcggtgg ccttgtgctt aaaacctgcg
     1981 gcccggccac agccttttgc catatcccca ccgcgcagat tactccggcc agtgtggccg
     2041 cctggtgtat cggccagctt aagggcgact tcggccccgg cgtttccagc attgaactga
     2101 actttcgccc ggagcaaacc gatggcccct tctaccacta cagccacggc ctgaaaaaac
     2161 acctgggcaa ttgtcagcgc atcgcccacg gtcatcgctc tattatcaag atttggcgca
     2221 atggccagct gtgcgagact agcatggagc aggttgccaa ggcgtggcgg gatatttata
     2281 tcggcacccg ggccgacata gtcgccgagc atgccgacca ctgggaattt gcctacagcg
     2341 caagccaggg caactttcgc ctgcagatcc ccaaagcggc ggtgtacctg atggatgagg
     2401 atactacggt ggaaaatatc agcgcccact tagctgccaa actcaaggct gaaagccccc
     2461 aggatcaact ggtggttaaa gcctatgaag gcctgggcaa aggggctgag gtgagctgtt
     2521 agccttatcg taaaaccagc catgcaggcc acaagcacat gcaccttgcc ctgccccctt
     2581 gaaaaaccac tgttgcgtca ctaaaccgtc tccctccgcc cgttcaacta gcgccagcta
     2641 atttagctcc atgtccttag ccgcactaca gccgtcacca atctgcgcca tactttcact
     2701 gacacccttt ttgaaccaac cacaggcaga cagcctttgg cctattgtct tagggatcat
     2761 cggcttgatc gccccagcta gctatttcca aacgcctctg agtttggtat tcaatcgtaa
     2821 gaagcttcct agcaatttcc acccgcgagt cagcatccat gagcagtagt acagccctcc
     2881 tgggctttaa tgacccgttt agcagctgga cacacctatt agcggcggtc ttcttcggca
     2941 tagcaacgtt tttcctgctc aagaagggca agggcaacgc cgtgcgcatc gccgccctgt
     3001 ctttgtactc ctttgccttg gtttttttat tttccatgag cggggtgtat cacctgctgg
     3061 cccaaacggg cgatgcccgc gccgttttac agcgcctgga tcacgccgcc atctgggtat
     3121 taattgccgg cacatttacc cccatccacg tcattttatt tcgcggcccc tggcgctggt
     3181 taattctctc aaccgtttgg ggcatcggcc tgctggggtt aatttttgag gcgatatttt
     3241 tcacatcctt tccccagtgg ttaattacca gcttattttt aggtctcggc tggatgggcg
     3301 cgcttaccgg ctataaattc cgcaaagcct atgcagggga aaaactgcgc ggtttagtgc
     3361 tgggcggtat tttttattcg atcggcgctg tgtgtgattt tttgcagtgg ccggtcatct
     3421 ggccctcgta catcggcggc cacgacgtgt tccatatatt cgtgattctc ggcgcgattt
     3481 cccactggcg gtttatttac aaatgggcgg cacactccgc cagcaacaaa atgattttcc
     3541 acgtttccac ctttccagat ggcgccgtgg tgggccaggc ggtgggggat cacattcggg
     3601 tggaggccag cgatctacac gagctcaaag cgcgcatcaa aaccctggtt aaaaccaaat
     3661 tccacagcag tatcgaaccg gaaattcatc tgaaatactt taacgaagag cgcattcagt
     3721 agcgcagtgg caagggccta ttggccccag gctcagcgtg acgtcgaatt aaccgcgccg
     3781 aaaaaagaaa accccaagga ccttgcgatc actggggttt ggattatggc ggtgagggag
     3841 ggattcgaac cctcgatacc ttgcgatata cacactttcc aggcgtgctc cttcagccac
     3901 tcggacacct caccaacgga aggtggtacc ccccgaaaag gagcgcaaat gtacacaagc
     3961 agccagcgga actcaagctg tttttaccga tagagataac aacttagata aatggcgatt
     4021 cttataccat acttggaatt agacctaagg gcatccgccc cggctttgct attgcattta
     4081 cggcgcacag aacaaaatga ctcatggaat cgcctaactt tttgcttatt ctcgcctggg
     4141 aagcattcgg cctagcctta gtagccgtag cagtgttgtt atttcaaaac cgcaaaatgc
     4201 gctccctggt caataagctg caagcgcgta tgcacgagtt ggtaggggat ttaaaaaaga
     4261 gccgggacga gctgaacaaa aagccgccgg tgcgggaaga gacctacctg cactacatca
     4321 atcaacactt agaacagact aaaaaacacc atttttcctt tcgcggcggc caggacatca
     4381 tgctggatct ggacccgg
//
LOCUS       DAOQGS010000347         4394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10659_length_4394_cov_4.771376, whole genome shotgun sequence.
ACCESSION   DAOQGS010000347 DAOQGS010000000
VERSION     DAOQGS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10659_length_4394_cov_4.771376"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PKZ52_12645"
     CDS             <1..1109
                     /locus_tag="PKZ52_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNG60664.1"
                     /translation="IHIELTDINVNLAVTHNQLSVQQGTAKDSGAGTLALSGGCPEIY
                     PAAACQLNLALAGLQIINNRQLEAKATGDIKATLQERQLALSGALNLSPTTLKLSNAF
                     GSSIKELVVEEINKRGQKPTGAFWPSPLIDVQWQLGANSQVRGRGLEAQVEGTLRTQG
                     PLNQLAYNGNFYTTKGTLELFNKRFILDTGDIRLAPGQIYLDMPAHYESRVNSGEDLT
                     IQARLQGDLNHLNLDLRSTPTLPPDEVLARLIFGKRIETITPFEGLQLAAAVNQLRAG
                     DGFNLMDSTRSLLGVDRLKIDSEKNTDGSTGVNVGVGKYISDKVYVEIQRTPNPNTPW
                     QGQIEVELTPSVNFESNTGQNGQGGAKLLWRRDY"
     gene            complement(1440..2681)
                     /locus_tag="PKZ52_12650"
     CDS             complement(1440..2681)
                     /locus_tag="PKZ52_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG60665.1"
                     /translation="MRPKSSLLSEKIRADRRQYLRLIAKGGALASLASTSPLAAAALA
                     NRLDETAPSKKFILIYHPGGGPNNYLNSVAIKPFEPFGDRVAALTMTVTWPGNHGLSF
                     MAGGANSWYPQQLNSSTLDQQIADTIGHRTPLRCLSLGVQTGGLPFIYRKGLPLPTID
                     TPQQTIDSIMRQFSAAPVKEGISRYDREYAQLSAHKQGLNELFKRIGNEERFKLEEHL
                     AALERYPQRIEQRNRLVSDGPCGAITYTPGTSPLNLYRAQGDIAVTALACGLTNVAAI
                     QFNNTQETWEANDGTPDAVPVVGDMKQVVNGGIHRNYYANLNEYMNKGVAHIVNQLIS
                     AGIFNDTVVLCVTEMGNTEQNTPEGGPITAATGISGFKGGARKLNNSHYAIFPDVVRL
                     LGLEWAVNETIYDYGAGGIIV"
     gene            complement(2922..3335)
                     /locus_tag="PKZ52_12655"
     CDS             complement(2922..3335)
                     /locus_tag="PKZ52_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG60666.1"
                     /translation="MDIGEVTQLTGLPASTLRYYEEKGLICSNGRVGLRRQYAEGVIE
                     RLGLIALARNAGFSLEEITALFSAGRSINRSLLLDKAAQLDKKIAELTAMRDGLRHAA
                     ACSAANHFECPKFLRLLNLAVKNPRRSTNKLKEVP"
     gene            3469..4272
                     /locus_tag="PKZ52_12660"
     CDS             3469..4272
                     /locus_tag="PKZ52_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG60667.1"
                     /translation="MLSIDWLLIYLALGIFVGFMAGLLGIGGGGILVPMLVSIFTYQG
                     FAPEHVVHLALGTALACMIVTASASLRAHALKGTVHWPLAFGLAPGIILGAYLMARSS
                     VTINPRAIALFFAIFMALIAVQMLLKWQPKSAPGSIKPHQLIFTGLGIGAISALATVG
                     GGFLTLSYLSYKNIDIKKAIGTSAAIGLPIAVAGTLGYISSGSSQAFNSPYTLGFVHW
                     PALCVLALSSMLAAPLGVRCASYLAPIYLKRIFALVSLALSVKMLVAVF"
BASE COUNT         1113 a         1172 c         1033 g         1076 t
ORIGIN      
        1 agattcacat tgaattaacc gacatcaatg tgaatctggc ggtaacgcac aatcaattaa
       61 gcgtgcagca aggcacagcg aaagattccg gcgcgggcac actcgcctta agcggcggct
      121 gcccagagat ttatcccgcc gccgcctgcc aattaaacct cgccctcgcc ggcctgcaaa
      181 ttattaataa ccggcaatta gaggctaaag ccactggcga cattaaagcg acgctgcagg
      241 agcggcaatt ggcattgagc ggcgccctca acctctcccc caccacctta aaactctcca
      301 acgcctttgg cagcagtatt aaagaattag tagtagaaga aataaataaa cgcggccaaa
      361 aaccgacagg tgcgttttgg cccagcccct taattgatgt gcagtggcaa ttaggcgcca
      421 acagccaagt gcgcgggcgc ggcttagaag cacaagtgga aggcaccttg cgcacccaag
      481 gtccccttaa tcaattggcc tacaacggta atttttacac caccaaaggc accctggagt
      541 tatttaataa gcgctttatt ttagataccg gcgacattcg cctagcgccg gggcaaattt
      601 atttggatat gcccgctcac tatgaatcac gcgtcaatag cggcgaggat ttaaccattc
      661 aagcgcgcct gcagggggat ttaaatcacc tgaatttaga cctgcgctcc accccaaccc
      721 tgccgccgga tgaagtctta gcgcggttaa ttttcggcaa acgcattgaa accatcaccc
      781 cgttcgaagg cctgcaatta gccgcagcag ttaaccaatt gcgcgccgga gacggcttta
      841 atttaatgga ttccacccgc tctttattgg gagttgatcg gctaaaaatt gattcggaga
      901 aaaacaccga cggcagtacc ggcgtaaacg taggtgtggg caaatatatt agcgataagg
      961 tgtatgtgga aattcaacgc accccaaacc ccaatacccc ctggcagggg caaatagaag
     1021 tggaattaac ccccagcgta aactttgaaa gcaataccgg ccaaaacggc cagggcggcg
     1081 ctaaattatt gtggcgcagg gattattaaa gtgcctctga acatctacgc ggcgaagcgc
     1141 tggctaggcg aacaggccaa aaaaagcggc gtttgcacgc agccaataag cattttttgg
     1201 agcattcaac aacacatggc gaatatgggt aatttttcag aggttcctta aaaaactgtt
     1261 tgccaaaaaa ctgcttaaac atctcaattt gcgcgctcta catacaacaa tcacaagacg
     1321 gctgttttga gcaactgcag agggcgggcg ctttagtaac tgcgggtgtt ctaaacctcg
     1381 cccatggcaa taacctgtat ccattactac tgcaccgcgc gctcatttcc ctaaccacct
     1441 cacacaataa tgccaccggc gccgtaatcg tagatggttt cattcaccgc ccattcaagc
     1501 cctaataggc gcaccacatc cgggaaaatg gcgtagtggc tattattgag tttgcgcgca
     1561 ccacctttaa aaccactaat acccgtggcc gccgtaattg ggccgccttc aggcgtattc
     1621 tgttcagtat tacccatctc ggtcacgcac aacacaacgg tgtcattgaa aataccggcg
     1681 ctgatcaatt gattaacaat atgggccaca cccttattca tatattcatt taaattcgcg
     1741 taataatttc tatgaatacc accatttaca acctgcttca tatcccccac caccggcacc
     1801 gcatccggcg tgccgtcatt tgcctcccag gtttcctggg tattattaaa ttgaatggcc
     1861 gccacattgg ttaaaccaca cgccagcgcc gtcacggcaa tatcaccctg ggcgcggtag
     1921 aggtttaatg ggctagtgcc gggggtgtag gttattgcgc cgcaagggcc atcactgacc
     1981 aaacgattgc gctgctctat acgctgcggg taacgctcaa gagccgcaag atgttcttcc
     2041 agtttaaatc gctcttcatt gccaatgcgt ttaaacaatt catttagccc ctgtttatgg
     2101 gcgcttaatt gcgcgtattc acgatcgtat cgggaaattc cctctttaac cggcgccgct
     2161 gaaaactgcc gcattatgct atcaattgtc tgttgtgggg tgtcgatagt gggcagcggc
     2221 aaacctttgc gataaataaa tggcaaacca cccgtttgca cccccaaaga caaacatcta
     2281 agtggtgtgc ggtggccgat ggtatcggca atttgctggt cgagggtgct ggaatttaat
     2341 tgctgcggat accaggagtt tgcccctccg gccataaaag aaaggccgtg attacccggc
     2401 caagtaacgg tcatggtcaa agcggcaaca cgatcaccga agggttcaaa gggtttaatc
     2461 gctacggagt tcaaataatt attcgggcca ccgccggggt gataaatcaa aataaatttt
     2521 ttgctgggcg ctgtttcatc aaggcgattg gccaacgcgg ccgcagccaa aggtgaggtg
     2581 gacgcaagac ttgccagggc gcctcctttg gcaattaaac gcaaatattg gcggcgatct
     2641 gcgcgaattt tttcactcag caatgaagat tttgggcgca taacaggctc ctcgttattg
     2701 tatacggcca catttgcctg cctaaaccga caaattccta acccgccccg gcaaaccgcc
     2761 agcaaacgag cacctgaata atataagcaa gctttgataa agaaaaattt atctagcttc
     2821 aatgtgatgg gagcctcaac gactattcgc cattacagct gcgccgttat ttatctgctt
     2881 tttagcggcc ccacatatcg tgactaggtg cttcactgcg gtcatggaac ctctttaagt
     2941 ttattggtgg atctgcgcgg attttttacc gctaaattca acaggcgcaa aaactttggg
     3001 cattcgaaat gattggccgc actacaggcg gcggcgtggc gcaggccatc gcgcatagcg
     3061 gtaagctcag caattttttt atccaactgc gcagccttat ctagtaataa actgcgatta
     3121 atactgcgcc ctgcgctaaa cagcgccgta atttcttcca agctaaaccc cgcattgcgc
     3181 gccagcgcaa ttaaacccaa gcgctcaatc accccttcgg catattgcct gcgcagcccc
     3241 acccgcccgt tcgagcaaat aaggcccttc tcctcgtaat accgcaaagt ggaggcgggc
     3301 aagcccgtca actgtgtcac ttcacctata tccattaccg ccccttgact taaagtcaac
     3361 tttaggttgt aggctaattc taagtgctac ccacagcact aaacaacacc cctaaccggc
     3421 tgcgcaggcg catcactctt ttttacacca cacattggcg gtttttttat gttgagtatc
     3481 gattggttat taatttacct cgccttgggt atttttgtcg gttttatggc gggtttattg
     3541 ggcataggtg gcggaggtat tttagtgcct atgctggtga gcattttcac ctaccagggg
     3601 tttgcaccgg agcatgtggt gcatttagca ctgggcaccg ccctcgcttg catgatcgta
     3661 accgcaagcg ccagcctgcg cgcccacgcg ctaaaaggca cggtgcactg gccattagcc
     3721 tttgggcttg cgccgggtat tattttgggg gcctatttaa tggcgcgcag ctcagttacc
     3781 attaacccac gcgccatcgc actattcttc gccattttta tggccttaat tgccgtgcag
     3841 atgttattga aatggcagcc caaaagtgca cccggcagca ttaaacccca ccaattaata
     3901 ttcactggcc tgggtatagg cgctatttca gcccttgcca cggtgggcgg tgggttttta
     3961 acgctgagtt atttaagcta taaaaatatc gatattaaaa aagccatcgg cacctcagcc
     4021 gccattggtt tacccattgc tgtagcaggc accctaggtt atatcagcag cggctccagc
     4081 caggcattta acagccccta taccttgggt tttgtgcact ggcccgcgtt gtgtgtactg
     4141 gcccttagca gcatgctggc ggcgccatta ggtgtgcgct gcgcaagcta tttagcaccg
     4201 atttatttaa aacgaatatt tgcgctggtg tccttggcgt taagtgtaaa aatgctggtg
     4261 gcggtttttt aatttttata cgcaccattg ggatggcgct gacagccgat tattaacccg
     4321 gcggcatgga tgccaccccg tgcaaaccgc cgtgagctgc cctaaaacaa ggaaatccac
     4381 gcattttcgc cagt
//
LOCUS       DAOQGS010000348         4390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10673_length_4390_cov_4.734025, whole genome shotgun sequence.
ACCESSION   DAOQGS010000348 DAOQGS010000000
VERSION     DAOQGS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10673_length_4390_cov_4.734025"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..4022)
                     /locus_tag="PKZ52_12665"
     CDS             complement(102..4022)
                     /locus_tag="PKZ52_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG60668.1"
                     /translation="MSIQLLLALILTCAAQWAAAVTSDPVYPLHLEQSYRQDYDLSVL
                     RTPDGFLWIISDNGLKRYDGYSLRQFNYDPNDPSSLGSPGGFKLFLDARNTLWVLGSV
                     LSRYLPESESFARYSVSNNRGLYTAADATGGNIWLGGERLGLQLFDPRLGEVRKRYLA
                     DTSPLTIFSILPQHDSERLWLATNVGLLLFDPASGEYDTYFQLPNTQGFQPLMSLAQD
                     QAGDLWVASREGLFRINPASRAVHNYPVDAQTPGALSTNALTAIGADRYNNIWIGTEK
                     MGVFRHDPTTDQFELYPASAFDALRFPPATIASIYEDPEGTMWFVCGQQGVVSVNRHL
                     QKFKSFRHSFDSDNSLGFNRVSGLFEDSKGFIWIGTDGNGVDRFNPTTFEFTHFRHNP
                     DDPETLASNAVLAIAEDSHGTIWLGTWAGGLNRLDPATGKVKRYLHDPAAKEGQSIAE
                     NHVMRIHIDNHDRLILAGVASGLQIFDPANGHFQAFTPNGKDASKGIQNYFINDIIPT
                     ADGSFWLGGYQTLERFSPATGYSLVPDLAINKGVLDLHQDKNGLLWIATNAGLYRFNP
                     TRNEVIPFTAADGLRDDFIVGIEQDSLDYLWLATRNGLVRMEQTSRAIDTFDELDGLA
                     GSDFNRFSHLTTRAGLMYFGTDKGISFFDPLHIPRNEYAPHIHFTQLQVNQQVQQLGK
                     SPWLARAFDLTQQLTLPDWADDIKITFAATNLVMPAKNRYRYRLLGKSDEWIDATDQH
                     SAQYSNLSPGQYRFQVIAANNDGVWTGTTRELIITILPAWWQTWWAYCLYAALFVASI
                     QGFSMLRLHLSTQRRKTLEHLVNEQTGKLKEANRAISQLNSQLEQRVAQRTRDLQAEV
                     EERKESEAKVTYIAYHDILSGLHNRAWLLHHLDSLLTAAHTNHDAPPFALLFIGGDRF
                     RAINDTYGHQTGDKLLILAAEKLREICPHPMQLARLGSDEFAVVIDQLNERSFAQQLA
                     ETIVNAFKEPIALDQLRLTLNVSVGYVIAGERYIDATQVLRDANIAMQKAKDRGRGIS
                     QEFDAEMLQQMLDQTALEADLKLALSRNEFSVVFQPIMRLSSMKAAAFEILIRWQHPS
                     RGMVPPDRFITMAESSGLIFDIGLWVLEQACQQLTLWKTTWPDQQLPIVAVNLSPIQL
                     EREDFLTRIEQIFQRTQIDPHQIEFEITESALMRQTNTVTSTLHELRQRGIQLGIDDF
                     GTGYSSLSYLDKLPVQILKIDRSFINALTQNNQINNNTSEIVRATIALAHNLKMSVVA
                     EGVETAEQLALLQSYGCDYGQGYYFARPLLAQDATAKLAQNFVQ"
     gene            complement(4019..>4390)
                     /locus_tag="PKZ52_12670"
     CDS             complement(4019..>4390)
                     /locus_tag="PKZ52_12670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG60669.1"
                     /translation="DHFKKLNDRYGHDMGDKVLRTFAAMLAMNLRDEDIFARLGGEEF
                     VVVCRRQPIEGVCAFADKLRRLASQCSFSADDDISISVSIGGAIVLAGEELSDTLKRA
                     DEALYTAKQNGRNRVEFDTRR"
BASE COUNT          965 a         1020 c         1287 g         1118 t
ORIGIN      
        1 atctacaaaa aacacgaaaa ttgatccagg ttacgcgttg atctattttg tcgccgggtt
       61 aataggtggc gcggtaataa gtttaattac cggggcagcg tttattgcac aaaattctgc
      121 gcgagtttgg ccgttgcgtc ttgagccaat agtggccgcg cgaagtaata accctggccg
      181 taatcgcagc cgtaggattg gagtagggcc agttgttcgg ccgtttctac gccttcagcc
      241 actacagaca tttttaaatt gtgggcaagt gcgatggtgg cgcgaacgat ttcactggtg
      301 ttgttgttga tttggttgtt ttgcgtaagg gcattaataa acgagcggtc tatcttcagt
      361 atttgtacgg gcaatttatc cagataactc aatgaagaat aacctgtacc gaagtcatca
      421 atgcccagtt gaattccgcg ttggcgcagt tcatgtagtg tggaggttac cgtgtttgtt
      481 tgccgcatta gggctgattc ggtaatttca aattcaattt gatggggatc tatctgggtg
      541 cgttggaata tctgttcgat acgcgttaaa aaatcctcgc gctctagctg gatgggcgac
      601 aggttaacgg ccactatggg cagttgctga tcgggccagg ttgtcttcca cagtgttaat
      661 tgttggcagg cctgttccaa cacccataag ccaatatcaa agattaagcc tgatgattcg
      721 gccatagtga taaatcgatc cggcggtacc atgccccgcg agggatgttg ccagcgtatt
      781 aaaatttcga aggcggctgc cttcatgctc gataggcgca tgatgggttg gaataccaca
      841 ctaaattcat tgcggctaag cgccagtttt aaatctgctt ccagcgctgt ttggtcaagc
      901 atttgctgga gcatttcggc atcgaattct tggctgatgc cgcggccacg atctttggct
      961 ttttgcatgg cgatgttggc atcgcgcagc acttgggttg catctatata gcgttcacct
     1021 gcaatgacat agccaacgct aacattgagg gttaagcgca gctggtctaa ggcgatgggt
     1081 tctttaaagg cgttaacaat ggtttcggct agttgttgtg caaagctgcg ctcgtttaat
     1141 tggtcgataa cgacggcaaa ttcgtcactg cctaagcggg cgagttgcat ggggtggggg
     1201 caaatttccc gtaatttttc agcggctaga atcaacaatt tgtcgcccgt ttggtggccg
     1261 taggtatcgt tgatggcgcg aaagcgatcg cccccgataa acaatagcgc gaaggggggg
     1321 gcgtcatggt tggtgtgtgc cgctgttaac aggctatcca ggtggtgtag taaccaagcg
     1381 cgattatgta aaccactcag aatgtcgtgg taggcaatgt aggttacttt ggcttcggac
     1441 tctttgcgct cttctacttc tgcttgtagg tcgcgggtgc gttgtgcaac ccgctgctcc
     1501 agttgggaat tgagttggct gatggcgcgg ttggcttctt ttaatttacc ggtttgttcg
     1561 ttgacgaggt gttcgagtgt tttgcggcgt tgggtcgata ggtgaagccg cagcatgcta
     1621 aagccttgga tggaggcgac aaatagggcg gcgtacaggc aataagccca ccaggtttgc
     1681 caccatgcag gcagtatggt gatgattagt tcgcgggttg tgccggtcca gacgccgtcg
     1741 ttgttggcag ctataacctg aaacctatat tggcccgggg ataggttgct gtattgggcg
     1801 ctgtgttggt ctgttgcatc aatccattcg tcgctcttgc cgagcagcct atagcgatag
     1861 cggtttttcg ctggcatcac aaggttggtg gcggcgaagg tgattttaat atcgtcggcc
     1921 cagtcgggca gcgtcagttg ttgggtgagg tcaaaggcgc gggcaagcca gggtgatttg
     1981 cccagttgtt gcacttgttg gttgacctgc agttgggtga agtggatgtg cggtgcatat
     2041 tcattgcggg gaatatgcag cgggtcaaaa aagctgatgc ctttgtcggt gccaaaatac
     2101 attaagcctg ctcgggtggt caggtggctg aagcgattaa aatcgctccc ggctaggccg
     2161 tccaattcat caaaggtatc gattgctctg gaggtctgtt ccatacgaac caaaccgtta
     2221 cgggttgcca accacagata gtccagggag tcttgttcaa tgccgacgat aaaatcgtct
     2281 cgcaaaccat cggcagcggt gaagggaatg acttcgttgc gggttgggtt aaagcggtag
     2341 agccctgcgt tggtggcaat ccacaacagg ccattcttgt cttgatgtaa gtccaaaaca
     2401 cccttattga tggcgagatc gggaaccagt gaatatcccg tggcaggtga aaatcgctcg
     2461 agggtctggt agccgcccag ccaaaaactg ccatctgcag ttgggatgat gtcgttgata
     2521 aaataatttt gaatgccttt ggaggcgtcc ttcccattag gtgtgaaggc ttgaaagtgt
     2581 ccgttggcgg ggtcaaatat ttgcaggccc gaggccactc ctgccagtat caagcggtcg
     2641 tggttgtcga tgtggatgcg catgacgtga ttttcagcta tagattggcc ctctttggcg
     2701 gctgggtcgt gtagataccg cttcaccttg ccggtagcag ggtcgagccg gttgaggccc
     2761 cccgcccagg tgcccagcca aatggtcccg tgggagtcct ccgcaatggc aagcaccgcg
     2821 ttggaggcca atgtttcggg gtcatctgga ttgtggcgga aatgtgtaaa ttcaaatgtc
     2881 gtcgggttga agcgatcgac accattgcca tctgtgccaa tccatataaa acctttgctg
     2941 tcttcaaata agccggaaac tcggttaaaa cccagggaat tatcgctatc gaagctgtgg
     3001 cggaatgatt taaatttctg cagatggcgg ttgacgctga cgacgccctg ttggccgcag
     3061 acaaaccaca tggtcccctc tggatcctcg taaatacttg caatggtcgc cggtggaaac
     3121 cgcaaggcgt caaaggccga tgccgggtat agctcaaact ggtcggtagt ggggtcgtgg
     3181 cgaaacacac ccattttttc ggtaccgatc caaatgttgt tatagcggtc tgcgccgatg
     3241 gcggttaatg cattggtcga taaggcaccg ggggtctgcg catccacagg atagttatgc
     3301 accgcgcggc ttgcagggtt gatacgaaac agcccttcgc ggctggccac ccataggtcg
     3361 ccggcctgat cctgcgccag gctcatcaag ggctggaacc cctgtgtatt gggcagctgg
     3421 aaataggtgt cgtattcccc gctggctggg tcgaataaca gcaggccaac attggtggcg
     3481 agccatagac gttcagaatc gtgctgggga agaattgaga aaatggtcag cggtgaggtg
     3541 tcagccaagt agcgtttgcg cacttcaccc agccgggggt caaacagctg taaacccaaa
     3601 cgttcgccgc ccaaccagat gttgccgcct gtggcatcag ccgctgtata aagccccctg
     3661 ttattgctga cggaatagcg tgcaaaggat tccgattcgg ggaggtagcg gctgagtacc
     3721 gaacccaata cccacagggt gttgcgtgcg tctaaaaata atttaaaacc gccgggtgag
     3781 cccaagctcg acgggtcgtt ggggtcatag ttgaattgcc gcaggctgta cccgtcatag
     3841 cgtttcaggc cattatcgga aatgatccat aaaaaaccgt cgggcgttcg caggacggaa
     3901 agatcataat cctgccgata ggattgctct aaatgcaggg gatagaccgg gtcgcttgtc
     3961 acggctgcgg cccattgggc ggcgcaggtg agtatgagtg cgaggaggag ttgaatgctc
     4021 atcggcgggt atcgaactcc actcggttgc ggccattttg tttggccgtg tagagggctt
     4081 catcggcccg tttgagcgta tctgataatt cctctccggc caggacaata gcgccgccga
     4141 tactgacgct tatggatatg tcatcgtcgg cgctgaaact gcattggctg gccagccgcc
     4201 gcaatttgtc ggcaaaggca cataccccct caatgggttg acgacggcag accactacaa
     4261 attcttcgcc gcctaatcga gcgaagatgt cttcatcgcg cagattcatc gccagcatgg
     4321 ccgcaaaggt gcgcaacacc ttgtcaccca tatcgtggcc gtatcggtcg ttgagctttt
     4381 taaaatgatc
//
LOCUS       DAOQGS010000349         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10748_length_4372_cov_5.307158, whole genome shotgun sequence.
ACCESSION   DAOQGS010000349 DAOQGS010000000
VERSION     DAOQGS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10748_length_4372_cov_5.307158"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..754
                     /locus_tag="PKZ52_12675"
     CDS             260..754
                     /locus_tag="PKZ52_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNG60670.1"
                     /translation="MPCARRIFAVALFALCLPQVSFSGEAAAESAGAVTKELLLARYD
                     VNGDQVISTEEISQKRDKAFAKMDLNQDGNVSMEEFTHIDELKRQPIVAARFNKLDSD
                     RDGVVSQEEYRTYLGSFKQFDQDGNGKITLAEMDAPRAAEPAKPAAKTHCLLWVCVRG
                     SLKD"
     gene            complement(1292..2101)
                     /locus_tag="PKZ52_12680"
     CDS             complement(1292..2101)
                     /locus_tag="PKZ52_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNG60671.1"
                     /translation="MRRVVFNQKGGVGKSSITCNLAAISAEQGLRTLVVDLDVQGNSS
                     YYLGLDIHAANVPSPEANIAQLLRQSASTWFASASPALSFVRETPFKNLDLLSASPLL
                     AQIAVELEARYKIYKLREVLDELNQHYDRIYIDTPPNFNFFSKTALIAADRVIIPYDC
                     DSFSKQGLNQLLENLVDLKQDHNPNLIIEGVVINNYTPQARLPNALIEQLKNEALPVL
                     ATYLNSSIKMKESHYACKPLIYFARSHKLTGQFKALFDELEAASEVKSTRV"
     gene            complement(2200..4371)
                     /locus_tag="PKZ52_12685"
     CDS             complement(2200..4371)
                     /locus_tag="PKZ52_12685"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNG60672.1"
                     /translation="MALPIEGMSCASCAGRIEKALLEVKGVVSAAVNLAAERAQVEML
                     KGGSRQALVEAVKKAGYSVAQTAAASPVAPPRVDGQLRKVFLAILLTLPLVLPMLLAP
                     FGIPLMLPGWLQLALAAPVQFWLGGHFYLAAWRGIRAGTGNMDLLVALGTSAGFGLSL
                     YQLTASHTSHGLPHYYFEASAVVISLVMLGKWLETRAKRQTASAIAALQQLQPTLAWL
                     RRDGQVVQVAVETIAPGDQILVKPGERVPVDGELLEGHSHIDESMLTGESLPVAKGPG
                     SKARGGTINLDGQILIHTEAVGSETTLAKIIRLVESAQAAKAPIQRLVDKISAVFVPV
                     ILALALCTFGRWWWLGGDVQQALINAVAVLVIACPCALGLATPTAIIAGTGVAARFGI
                     LIQNAESLEIAFRVTHVVLDKTGTLTQGAPSLVAQIPARPDAPLLAIAASLQTASTHP
                     LAKAVLAQAQSDGLKLAAVSEAQVLPGRGIAGVVNGEAFRLGTRRLMHELGIDTQPLQ
                     GAAEQYESQGQTISWLAQLGPEQKLLGLLAFGDTIKPSAAEAVQRLHALGIQTLLLTG
                     DNQGSADYVAKRVGINKVIANVLPADKAQVISELKDAGHSVAMVGDGINDAPALAAAD
                     VGIAMASGADIAMHTASITLMRNDPALIADALDISRRTYRKIQQNLFWAFIYNLIGIP
                     LAAFGYLNPMLAGAAMAFSSVSVVSNALLLKRWRPAQARGE"
BASE COUNT          971 a         1160 c         1169 g         1072 t
ORIGIN      
        1 cagttgaact ctagtatggc cgctaatgct cgaatctgcg cgggtttaag agaatcacac
       61 gccggataag tgtcgcttgc ccctagtatc ccgccgtatc ttaatcacat agattgccgg
      121 gcagggctgt tgaccgtttg gaaggcacaa tgccgacttg gtcgcaaagc caatggatgc
      181 cctacttaag tgtgaattct ctgctagatt ttattggtaa ttggaatatg atccatccaa
      241 ctaccccgga gattccccta tgccttgtgc ccgccgtatt tttgcagttg ctctatttgc
      301 cctgtgtctg cctcaagtca gttttagcgg agaagcggcg gcggagtctg ccggtgcggt
      361 cactaaggaa ttgttgctgg cgcgttatga tgtcaatggc gatcaggtta tttctaccga
      421 agaaatcagc caaaagcgcg ataaggcatt cgccaagatg gatcttaacc aggatggcaa
      481 tgtaagtatg gaggagttta cccatatcga tgaactcaaa cgccaaccca tcgttgctgc
      541 gcgttttaat aagcttgata gcgaccgcga tggtgttgta tcccaagagg aataccgcac
      601 ctatttaggc tccttcaagc agtttgatca agacggcaat ggcaaaatta ccctcgctga
      661 aatggacgca ccccgcgccg ctgaacctgc taaacccgct gctaaaacgc actgtttgtt
      721 atgggtgtgc gtgcgcggta gcctcaaaga ttaaagggca cctgcggaat tttcctattc
      781 gcgcccgagc ggcgcgccat attttttgtg ctcagtcggt catttatcca gtacaaaacg
      841 ccccttcgat tttatgcttg gtgaaattct tgggattcct gcttttgatt ctttgcagtt
      901 atcctaatcc tcattgatag ccgaggcagg ttagggcaac cacaagggtt gcccctacta
      961 tcgcgccttt acttgttaac cagcggcaaa atagatcgtc ctgatttatt tttccttcgc
     1021 cgcgataaac ttacgaaaag gcgtggattt cggtgtttta tcgcagctcg caggcggcct
     1081 gaaccgccga gagcggcatc cttgccgcta ttattactag ctgtgcggaa gcaatgaata
     1141 cacatcattt cagaccgtca aaaagaggga cctttttgac gagcctacac ggatgtattc
     1201 acggcgtgtc tgaaatgatg tgtattcatt gcggatgtct aggtagtaac ccggcactaa
     1261 atgccgccgg gttaataata ccatttgccg cttacaccct agttgattta acttcactgg
     1321 ccgcttctaa ctcgtcaaat aaggctttaa attgccccgt gagtttgtgg ctgcgggcaa
     1381 aataaatcag cggtttgcag gcgtagtggg attctttcat tttaatagag ctgtttaaat
     1441 aggtcgccaa aacgggcagg gcttcatttt ttagttgctc aattaaagcg ttgggcaggc
     1501 gcgcttgggg ggtgtaatta ttaatgacca cgccctcaat gattaaatta gggttgtggt
     1561 cttgttttaa atccaccagg ttttccagta gttggttaag cccctgtttg gaaaaactgt
     1621 cgcagtcgta ggggatgatt actcggtcgg cagctatgag ggcggttttg ctaaagaaat
     1681 taaaattagg cggtgtatca atataaatgc ggtcgtagtg ttggttcagc tcatctaata
     1741 cttcgcgcaa tttataaatt ttatagcggg cctctaattc caccgcaatt tgtgccagca
     1801 gcgggctggc gcttaataaa tctaagtttt taaagggtgt ttcccgcaca aaacttaacg
     1861 ctgggctggc gctggcaaac caggtggatg cgctctgccg cagcaattga gcaatattgg
     1921 cctcggggct tggcacattg gcggcgtgaa tatccaggcc cagataataa ctactattgc
     1981 cctgcacatc taaatccacc accagggtgc gcaggccttg ctcggcgctg atggccgcta
     2041 aattacaggt aatactggat ttacccacgc cgcctttttg gttaaatact acccgtctca
     2101 tcgcgcgcct ctttattttg gttggtgtga aatcgattgc gaacaactgg tgaaatgaga
     2161 ttccccagtt gtcttttaaa atcgtaccgc cgcgggcatt tattcacccc gtgcctgcgc
     2221 cggtcgccag cgtttaagaa gcagggcatt acttaccacg ctgacactgc taaacgccat
     2281 ggccgcgcct gccagcatcg gatttaaata gccaaatgcc gccaagggaa tgccgataag
     2341 attgtaaata aaggcccaaa ataaattttg ttgaattttg cggtaagttc gcctggaaat
     2401 atccaaggca tcggcaatta aggctgggtc gttgcgcatt agggtaatgc tcgcggtgtg
     2461 catggcaatg tcggcgccgg atgccatggc aatacccaca tcggcagcgg ctaaagccgg
     2521 ggcgtcatta atcccatccc ccaccatagc cacgctgtgc ccggcgtctt taagctcact
     2581 tattacctgc gctttgtccg ccggtagcac attggcaatt actttattaa tgccaacacg
     2641 ctttgccacg taatcggcgc tgccctggtt atcgccggtt aacaacaggg tttggatgcc
     2701 tagcgcgtgc aagcgctgta ccgcctcggc ggcgctgggt ttgatggtgt cgccaaaggc
     2761 caataggcct aggagttttt gctcagggcc taattgcgct aaccaggaga tggtctggcc
     2821 ttgggattca tattgctcgg ccgccccctg cagtggctga gtatctatac ccagctcgtg
     2881 cattaagcga cgggtgccaa gcctaaacgc ctcgccatta acgacacctg cgatgccgcg
     2941 cccgggtaac acttgtgcct cactgacagc cgccagcttt agcccatcgc tttgggcctg
     3001 cgcgagtacg gctttggcca atggatgggt gctagccgtc tgcaggctgg cggcgattgc
     3061 cagtaacggt gcatcggggc gggcgggtat ttgtgccacc aggctggggg cgccctgggt
     3121 gagggtgccg gttttatcca ataccacatg ggtaacgcga aaggcgatct ctaaactttc
     3181 agcattttga atcagtatgc caaaccgggc ggccacgccc gtcccggcaa taatggcggt
     3241 aggtgtggcg agccccagag cgcaggggca ggcaatcacc agtacggcca cggcgttgat
     3301 aagcgcctgt tgtacatcgc cgcctagcca ccaccaacgg ccaaacgtgc acagggcaag
     3361 ggccaaaatg acgggcacaa atacagcgct aattttatcc accaggcgtt gaatgggggc
     3421 tttggccgct tgagcgctct ccaccaggcg aatgatctta gcgagggtag tctcactgcc
     3481 aacggcctcg gtgtggataa gaatttgccc atctaaatta atggtgccgc cgcgggcttt
     3541 actgccgggg cctttagcca cgggcaagct ctcgcctgtg agcatggatt catctatatg
     3601 gctgtgtccc tccagcagct caccatccac cggcacccgc tcacccggtt ttaccagtat
     3661 ctgatctccc ggcgcgatgg tttccaccgc cacctgcacc acttggccgt cccggcgcag
     3721 ccaggccagg gttggttgca attgttgtag ggcggcgatg gccgaggcgg tttggcgttt
     3781 ggcgcgtgtt tccagccatt tacccagcat caccaggctg ataaccaccg ctgaggcttc
     3841 aaaataataa tggggtaggc catggctagt atggctggcg gttaattggt agaggctgag
     3901 gccaaacccg gcgcttgtgc ccagagcaac cagcagatcc atattgcccg tgcccgccct
     3961 tatgccccgc caggcggcga ggtaaaagtg gccgccaagc caaaactgca ccggcgccgc
     4021 cagcgctaac tgcaaccagc ccggcagcat caatggaatg ccaaagggcg cgagcaacat
     4081 gggcagtact agcggcagcg tcagtaagat cgccaagaaa actttgcgca attgcccatc
     4141 aaccctgggg ggcgcgactg ggcttgcggc tgcggtttgc gcgacgctgt agccagcttt
     4201 tttcacggcc tcaactaagg cttggcggga gccgccttta agcatttcta cctgcgcccg
     4261 ctcggcggct aagttcaccg ccgcgctgac cacgcctttt acttccagta gggccttttc
     4321 gatgcggcca gcgcaggagg cacagctcat tccttcaata ggcagagcca cc
//
LOCUS       DAOQGS010000350         4363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10786_length_4363_cov_4.981894, whole genome shotgun sequence.
ACCESSION   DAOQGS010000350 DAOQGS010000000
VERSION     DAOQGS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10786_length_4363_cov_4.981894"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..1397
                     /locus_tag="PKZ52_12690"
     CDS             636..1397
                     /locus_tag="PKZ52_12690"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HNG60673.1"
                     /translation="MSLTKQSLIKGLCLAGFACVSLNTLAADREIGGYVDKAEDRFVR
                     NVWNFVKNFQSWQPVGSHRYLNTQYYYNQPYMMTTSHQNYVDKMDLAYIAGHGSSYYI
                     ETNKSLGQGVDLRNAPAYGDLATGGDLEFMIIESCYTVTSRPETTNWAAPYANMFRGL
                     HQLVGFHTLSNSDNGIPNNYAHKLKANGGVWQSWFAAVNEERYWIFNPTNSDGSPYPG
                     LASAIMYSSTENDRLGSYAADPAGGTSGMKTWWQY"
     gene            1448..2395
                     /locus_tag="PKZ52_12695"
     CDS             1448..2395
                     /locus_tag="PKZ52_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60674.1"
                     /translation="MKISSLVQAFLLVGATFNAQSLLAAGPDLALYQFARTPVKTVDS
                     TLASMKQFIGETPTAAKADDDNTVRFVSDKDVNTTFEQNLATGDLRFQRNFSAYLGDF
                     KPTLPTSDEAIKIAQTFLAANGLQAANERELTVAHVGGLRASSMIGDKPGPVIDKLVT
                     VNFARQVNDLPVIGPGSKMVVNIGNKGEIIGVIRHWRELAGSKKVSGADTYSIDEAKR
                     IALAQMSKEFGSTSDFEILQSKIAYYDNNGVYLQPVYAFETRVVNHELGDTFNYVSVV
                     PAIINAPEALNLRQTEQIAKDLITRNESVKPSTITKGFD"
     gene            2970..3845
                     /locus_tag="PKZ52_12700"
     CDS             2970..3845
                     /locus_tag="PKZ52_12700"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG60675.1"
                     /translation="MSHTLTRWHRLLNYIDAHLEETLGSEQLCKLAHFSQFHFHRQFC
                     AHFGVNLADYIQRVRFTQAAYLLAFRHQFTVTEIALMVGFNHPEVFSRAFKRWSNLTP
                     SAFRAQPPWEFIHQQNTQLNQIRGPFMHTDTANYTVELIQFPTLSLAVLPHRGAPDQL
                     GNSLLNFINWRKQHGLSPDKTRTFNIAYTPLDIEPQTDFRLDLAVELSHQVPLDHPDM
                     ERGTIAGGLCARIIHVGDDEGLEPAVLYLYGPWLSGHGYELRDAPLFLERKTFYPAVP
                     MHLRETHIYLPLQEK"
     gene            4082..4252
                     /locus_tag="PKZ52_12705"
     CDS             4082..4252
                     /locus_tag="PKZ52_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60676.1"
                     /translation="MQLINASLCSIANSDGITYEYVRLIYKSGQLILGYSILPHRVSK
                     ELLALVKNMDKK"
BASE COUNT         1223 a         1117 c          939 g         1084 t
ORIGIN      
        1 ggagcaattg ccgagtttac acgcagtaaa tgagcatttt ttgccaaatt caacgccgcc
       61 agggtgagta aggtaatttt tcagaggttc cctagcaata ctgcacctgc agaagtaact
      121 agaggttagt gcagacacat tgtgactaca gccgtgtagg ctcgtcaaca agattcctct
      181 ctttgacgat ctgaaacgat gtgtattcac tgctttcgta cagcgagtaa ttaattgcgt
      241 tctatttata ttagtttgag cagagcgcaa cgcccatgcg gctgaacatc ccatacaggt
      301 aaactcaatc aatacctacc aaaatccatt tacaccaccc ccgcgataga gcaacacgcc
      361 ttgttatcct gtgtattcat aaccggtaac cccgcgcccc gttcctgcgg ccccaataac
      421 cacagggtaa aaaatcacac gttcacagcg taaataatca ctgccaccca aaaggccttg
      481 ccctaaagta acacccactt cggcgctgct tgtacatcca caaggactta agtattagct
      541 cgggacagga ttagaggcaa gcggcatcag aaggaaatca aaggcaagga gttaatcaac
      601 acactgcctt caataaccca acattggaga acattatgtc acttactaaa caatcattaa
      661 ttaaaggatt atgcctcgca ggttttgcct gtgtttcatt aaatacactg gccgcagacc
      721 gtgaaattgg cggttatgtg gacaaagctg aagatcgctt tgtgcgcaat gtgtggaatt
      781 ttgtaaaaaa tttccaaagc tggcaaccgg tgggctcaca ccgctattta aatacccagt
      841 attactacaa ccaaccctac atgatgacga catcacacca aaattatgtc gacaaaatgg
      901 acctggccta tatcgcaggc cacggcagct cttattatat cgaaaccaat aaaagcctgg
      961 gccaaggcgt tgatttgcgc aatgcgcccg cttatggcga tttagcgact ggcggtgatt
     1021 tggaattcat gattattgaa tcctgctata ccgtaaccag ccggcctgaa accactaact
     1081 gggcagcgcc ttacgccaat atgttccgcg ggctgcatca attagtaggt tttcacaccc
     1141 tgagcaattc cgataacggc attcccaata attacgctca taaattaaaa gccaacggcg
     1201 gcgtgtggca atcctggttt gcggcagtga atgaggagcg ctactggata tttaacccca
     1261 ccaactcaga cggctcccct tacccaggtt tagccagcgc cattatgtac agctcgacgg
     1321 aaaatgaccg tttaggcagt tacgcggcag atcccgctgg cggcacaagc ggcatgaaaa
     1381 cctggtggca atattaattc tttttaaaac cctttttact tacattttga tgaacaggaa
     1441 atttcacatg aaaatctctt ctttagtaca ggctttttta ttggtaggtg caaccttcaa
     1501 cgcgcaatcc cttttggcag caggccctga tcttgcgctc taccaatttg cgcggacacc
     1561 ggtaaaaacc gttgatagca cgcttgccag catgaaacaa tttatcggcg agacccccac
     1621 tgcggcaaaa gctgatgatg acaacaccgt gcgttttgta tccgacaaag atgtcaacac
     1681 caccttcgag cagaatttgg caaccggtga tttgcgcttt caacgcaatt tctccgctta
     1741 cctaggcgat tttaaaccta ctctgcccac aagcgatgaa gcaataaaaa ttgcccaaac
     1801 ctttttggca gccaatggcc tgcaagcggc caatgagcga gaactaacag tagcccacgt
     1861 gggcggcctg cgcgcatcct ccatgatcgg cgataaaccc ggcccagtga ttgataaatt
     1921 ggttaccgta aattttgctc gccaagtgaa cgacctgccg gtaatcggcc ccggctctaa
     1981 aatggtggtt aatattggca acaaaggcga aattattggc gtaattcgcc attggcgcga
     2041 gcttgctggc agcaaaaaag tgagcggcgc tgatacctac tcaatcgatg aagccaagcg
     2101 catcgcttta gcacaaatga gcaaagaatt tggcagcacc tctgactttg aaattctgca
     2161 aagcaaaatt gcctactacg ataacaacgg tgtttacttg caaccggtat acgcatttga
     2221 aacccgcgtt gttaaccatg agctgggtga tacctttaat tatgtcagcg tagtgcccgc
     2281 cataattaat gcgcctgaag cattgaattt gcgtcaaact gagcaaattg ccaaggattt
     2341 aattacccgc aacgagagcg tcaaacccag cactatcacc aaaggttttg attaatctgc
     2401 caccgggaaa cccgtcgcac aaaaagtgta gcaactgccc catcaaaaac cccggccata
     2461 cgccggggtt ttttaatgtg tatttaattt attgtaatta ttcggcgtct ttattaaccc
     2521 agcactaaat gccggcgggt taataatcct aactaggact aaactcttca gcacacctta
     2581 ttaccctgtc cgcacgggcg gcaaattctg gaggatttag ccatactgca taatcgattc
     2641 ccagtgtgac agcgaagact ataggttatt atcggccgtc aaacgaggga cctttttgac
     2701 gagtctatcc agatgtattc acaacatgtc ggcactcacc tgtagtcacc actgcaccgg
     2761 gagcactggg ggcacagagc actaaaacac gcacctggta gtcgccaatc agtgcggcaa
     2821 tcaagcaccg ccgcataaaa aggtgccgaa tggttacata ggttatttga ccgggccccc
     2881 aagtgcgcaa aataaatcaa gccataactc aacaatggcg ctaacctgca gcaactggat
     2941 gtgaattaac actgcttatg aggaccaccg tgtcgcacac actaacccgc tggcataggc
     3001 tgctcaatta tattgatgca cacctagagg aaacgcttgg cagcgaacag ctttgtaagc
     3061 ttgcgcattt ttcgcagttt cattttcacc ggcaattttg cgcgcacttt ggcgttaacc
     3121 tggcggacta tattcaacgt gtgcgtttta cgcaggcagc ttatttattg gcttttcgcc
     3181 atcaatttac cgtaacggaa attgctttaa tggtgggctt taatcacccg gaagtatttt
     3241 cccgcgcctt taaacgctgg agcaacctta cccccagcgc atttagagcg cagccgccct
     3301 gggaatttat tcatcaacag aatacacagc tcaaccaaat tagaggcccc tttatgcaca
     3361 ccgatacagc caattacaca gtggaactca tccaattccc caccctgagc ctcgccgttt
     3421 taccccaccg gggcgcacct gatcaattgg gcaatagcct gcttaatttt attaactggc
     3481 gcaaacaaca tggtttatcc ccggataaaa cgcgcacctt taatatcgcc tacacaccgc
     3541 tggatataga accccaaaca gactttcgcc tggatttagc ggttgaatta agccaccagg
     3601 tacccctgga tcaccctgat atggagcgcg gcactattgc aggcggactt tgcgcccgca
     3661 tcatccacgt aggcgatgac gaaggtttag agcctgcggt gctgtattta tacgggcctt
     3721 ggttgagtgg acacggctac gaactaagag atgcgccact atttttagag cgcaaaacct
     3781 tttacccggc agtgccaatg cacctgcggg aaacccatat ctatttgccg ctgcaggaga
     3841 aatagatatg ggttacggag cttaaatgtt cttggtgctg cgacattaac ctgtggcatg
     3901 ccgcgccaag ccacatgatt atttctcctg aaacaagggt ttcttacggt ccggatagct
     3961 ttaagttcgc tttagaacaa agatatggcc ccttcagcga gtggtacacg tacctaagga
     4021 tgccaattct ataatcgctc ctaccggcac aaccgtgacc tttttatata ggagttatct
     4081 tatgcaatta atcaacgcgt ccctttgcag cattgccaat agtgacggaa tcacctacga
     4141 atacgtgcgc ttgatctaca aatccggtca attgatactc ggctatagta ttttgccaca
     4201 tcgagtcagc aaagaattgc ttgcacttgt gaagaatatg gacaagaaat aattataatc
     4261 caatatggag aactgtcagc cacacatttc acaatcaact gcaaagtact cagttagcaa
     4321 accacatcgt tggtctgcta actaaaagtt tttagttatc gcg
//
LOCUS       DAOQGS010000351         4359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10805_length_4359_cov_4.794610, whole genome shotgun sequence.
ACCESSION   DAOQGS010000351 DAOQGS010000000
VERSION     DAOQGS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10805_length_4359_cov_4.794610"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PKZ52_12710"
     CDS             complement(<1..1158)
                     /locus_tag="PKZ52_12710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNG60677.1"
                     /translation="MSQTSNNLASESWAIADLLRGDFKQSQYGRIILPFCLLRRLECV
                     LAPTKEAVLAKLHEIQPLNLPEEAHEKMLLRATQIGQNQGGLQFFNTSKMDLSKLGES
                     GIKANLENYIQGFSKDAREIFEHFKFMEFVGLLNDANLLYKVVQKIRTIDLSPSAISN
                     HDMGLVFEELIRRFAEGSNETAGEHFTPRDIVRLTTSLVFMEDDDALTKPGIIRTIYD
                     PTAGTGGFLSAGMEYVHELNPQAVMRAYGQELNPESYAICKADMLIKGQEVGNIKLGN
                     TLSNDHLYADKFDYMLSNPPFGVDWKKIEGDIQDEHKLKGFDGRFGAGLPRVSDGSLL
                     FLLHLISKLRDAKEGGGRIGIILNGSPLFTGGAGSGESEIRRYILEADLLEA"
     gene            1459..2505
                     /locus_tag="PKZ52_12715"
     CDS             1459..2505
                     /locus_tag="PKZ52_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60678.1"
                     /translation="MLKPPPCVHIAATSLLTPLGNTPECILVAIKAGVSAYAASAYSN
                     HAGQPMTITQVPEDALEPLTGELKGARLASRVLRLARLALPPLKQVMKSYTGNHPIPL
                     FLVGPEILPNRAQPVSSHLIHCLLQDESLLISRAKCRYFASGRTGLLEAIDLAFQCFE
                     QTNEHAILVGAVDSYIDAATLTQLDQEGRIAAGQNCDTFVPGEGAGFLLLCRPGSAEP
                     ATPSVFRPALGFEPGHKYSDSPMLGQGLDLAFKQALARLPGVSIDDIYSTMNGESWFA
                     KEFGIAFMRSSNRFTAEVKHHHPIDALGDMGSATPVVLLAMAVDNLLKRNTGASIVYA
                     SADTGNRAAVVLAY"
     gene            2554..3276
                     /locus_tag="PKZ52_12720"
     CDS             2554..3276
                     /locus_tag="PKZ52_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60679.1"
                     /translation="MTEIGEGIAVVIQRLEQDKCVFCGKADHKELNKEPINPTGWTRA
                     EISGVGGNFVAKKKSIYPSNISPPAAYRSEGHHCLAFSSFIVDARTSPKDRFAALNHY
                     LKQEGYNPNNPNNTIDLPGRKSKGDTTANANFKEFELAVLAGKPLQLHIGGHKQEFMS
                     ASNMLILAVVTALQDSNACKKPPDDFKKKLKQKIIDAEDRAFKFTASTTSPWVAHPGP
                     LKLAEAYVKKKHDIDSITYPQL"
     gene            3481..3870
                     /locus_tag="PKZ52_12725"
     CDS             3481..3870
                     /locus_tag="PKZ52_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60680.1"
                     /translation="MASEAFVHVLRNNGIDNIEVFPASIRLTEQSEPVHGFYAINVLG
                     IVDVIDQDNSIMDFENEDGNLAIYFIDKLVLKPECDSHALIYRPRGYRTRILIADRFK
                     QLLDQGGVVGALLVTPEKWDGFNGEKP"
BASE COUNT         1101 a         1052 c         1074 g         1132 t
ORIGIN      
        1 tgcctctaat aaatcggctt ctaaaatata gcggcgaatt tcgctttcgc cgctgcctgc
       61 gccgccggta aataagggtg aaccattgag gatgatgccg atgcggccgc cgccttcttt
      121 tgcatccctt aatttactaa tcagatgcaa taaaaataat aaggagccat cggacacacg
      181 cggtaagcct gcgccaaagc ggccatcaaa ccctttgagt ttgtgttcgt cttgaatatc
      241 gccttcgatc tttttccaat ccacaccaaa tggcgggttg gagagcatgt agtcaaactt
      301 gtcggcatag aggtgatcgt tagagagggt gttgcccaat ttaatgttgc cgacttcttg
      361 gcctttaatc agcatatcgg ctttacagat ggcgtaactc tccgggttca gctcttgccc
      421 gtaggcgcgc atgacggctt gcgggttaag ttcatgcaca tactccatgc ctgcagaaag
      481 aaatccgccc gtgcctgcgg ttgggtcgta gatggtgcgg atgatgcctg gcttggttag
      541 cgcgtcatcg tcttccataa ataccagcga ggtagtgagg cgaacaatat cccgtggggt
      601 gaagtgctca ccggcggttt cgttggagcc ttccgcaaag cggcgaatta actcttcaaa
      661 caccaagccc atatcgtggt tggagatggc gctggggctt aaatcaatgg tgcgaatttt
      721 ttgcaccacc ttgtacagca gattggcatc gtttaataag cccacaaatt ccatgaattt
      781 gaagtgctcg aatatttcgc gggcgtcttt agagaagcct tgaatatagt tttccaagtt
      841 ggcttttatg ccggattcgc ccagtttgga gagatccatt ttggaggtat tgaagaattg
      901 cagcccccct tgattttgtc cgatttgggt agcacgcagc agcatttttt cgtgcgcttc
      961 ttctggcagg ttcagaggct ggatttcgtg caacttggcg agtacggctt cttttgttgg
     1021 cgcgagtaca cactctaaac ggcgcagtaa acaaaacggc aggatgatgc ggccatattg
     1081 gctttgcttg aaatcgccac ggagtaaatc ggcaatggcc caggattccg aggctaggtt
     1141 gttggaggtt tgggacatgt gggtaattct taacctgagt tatgggtaaa cctgtgccct
     1201 gcgccctgtt gagcggtggc acggcttatt atcgtttgtg cggaatagta ccccagaggc
     1261 tagttgaggg ctatggtcgg ccgtctcgta agtagtaagg tatcgattga tcgcgcgcga
     1321 accctactca agtaccattt tgcgcagctc ccctttggta tctttagcaa aaatagcctg
     1381 caactggccc tggcctaggc tgaagcctta acataacaag ctccattctt ttctgtcagc
     1441 ccatgagggg ctgtagcaat gcttaaacca ccgccctgtg ttcacatagc cgccaccagc
     1501 ctactcaccc ctttgggcaa tacacctgag tgtatattgg tggcgattaa ggctggggtg
     1561 agcgcctatg ctgcgtcggc ctactccaat cacgcgggcc agcccatgac cattactcag
     1621 gtgcctgagg atgcgttgga accgcttaca ggtgaactaa agggggccag gctcgccagc
     1681 cgagttctgc gcctggcgcg cttggcactc cccccattaa aacaggttat gaagagctat
     1741 acaggtaacc accccattcc gctattttta gtaggccccg agattttgcc caaccgggca
     1801 caacctgtat ccagtcattt aattcattgc cttctacagg atgaatcgct gcttatttca
     1861 cgggctaaat gccgctactt tgcctctgga cgaacgggcc tactcgaggc gattgatctg
     1921 gcgttccagt gcttcgagca aaccaatgaa catgctattt tggttggcgc tgtagatagc
     1981 tatatcgacg cagctacctt aacccaactc gatcaagaag gacgcatagc agcggggcag
     2041 aattgcgata cgtttgtgcc cggtgagggc gccggatttc ttttgttgtg ccgacctggg
     2101 agtgcagaac ctgctactcc ttcagtgttc cggcctgctt tgggctttga acccggccat
     2161 aaatacagcg attcacctat gttgggccaa ggattagatt tggcgtttaa acaagcgcta
     2221 gcgcgtctgc ctggggtgag cattgacgac atttattcaa caatgaatgg ggagtcctgg
     2281 ttcgctaagg aatttggcat tgcctttatg cgcagctcaa accgctttac agcagaggta
     2341 aaacatcacc atccaattga tgccctgggc gatatgggct ctgcaacgcc agtagtttta
     2401 ctcgccatgg ccgtggataa tttactcaaa cgaaatacag gtgccagcat agtttatgct
     2461 tcggcagaca cgggcaatag ggcggcggtt gttctggcgt actgagaacc cacgttcatt
     2521 ttttgtgaag acttagcaat tgggagattg tacatgactg aaattggtga aggtattgct
     2581 gtagttattc aacggctgga gcaggataaa tgcgtgtttt gtggcaaggc cgatcataaa
     2641 gaattaaata aagagccgat aaaccctacc ggctggacac gcgcggagat ttccggtgta
     2701 gggggtaatt ttgtggcaaa gaaaaagtcc atttatccct ctaatatttc accaccggca
     2761 gcctaccgct cagaaggcca ccactgtttg gctttcagct cttttattgt agatgcccgc
     2821 acatcgccaa aagaccgatt tgccgcttta aatcactacc ttaaacaaga aggctacaac
     2881 ccaaacaatc caaacaacac catcgattta ccgggcagaa aatctaaagg tgatactact
     2941 gcaaacgcaa actttaaaga gtttgaatta gccgtgttgg cgggcaagcc cttgcagctg
     3001 catattggtg ggcataaaca ggagttcatg agcgcatcta acatgctgat tttggcagtg
     3061 gttacagcac tccaagacag caacgcctgc aagaaaccac ctgacgactt taagaaaaaa
     3121 ttaaagcaaa aaattattga tgccgaggat agagcattta aatttacagc aagtaccacc
     3181 tctccctggg ttgcacatcc tgggccgctt aagcttgccg aagcttatgt taaaaagaag
     3241 cacgatatag attccatcac ctacccacag ctataggtag tttccatgca catactttgc
     3301 cctaacactg cagtttcacc ggacttaacc tacagcgcga atacctcgtg caatgaactc
     3361 atctggctga cggggcaccc gatccctaag gaacatgatt taagcaattt agaattcctc
     3421 tgtgaaagcc tacccagtgg aaaattaacg gactttacaa tcagtgatgt aggctgtccc
     3481 gtggcatctg aagcttttgt acatgtgttg cgcaataacg gtatcgataa tatagaggtc
     3541 ttccctgctt ccattaggct gactgaacaa tccgaacctg tgcacggctt ttacgccatt
     3601 aatgtactgg ggattgttga tgtaattgac caagataatt caataatgga ctttgaaaat
     3661 gaagatggaa acctggcgat ttacttcatc gataaattag ttctaaaacc agagtgtgat
     3721 agccatgcat taatttatag gccacggggc taccgcacac gaattttgat tgctgatcga
     3781 tttaaacagt tgctggatca gggtggtgta gttggggcgc ttttggtgac acctgagaag
     3841 tgggatgggt ttaatgggga gaagccctga cccgagctac cctcgcggca attacaatta
     3901 atattgtgat tgcgcatttc cattcagcag gggtgcaaat taaattagcg gaactagccc
     3961 aaatactaat agcgctaata cgctgcatca aaaccaccgt gacaagtact gccaccgcca
     4021 gaacatccta aaagagaccg tataccttag cttcttcgaa acgtggatgc tgggcaccga
     4081 gtcgcacatc aagccaggtg tatttattaa cccggcgaca aaatagatcg tcctgatcta
     4141 ttttgtcttc gcccactaat cctgcgccga tgcgtatatc tgtgcaggat tagtggggct
     4201 cacgacggct tgggccgtcg gtgcagcttt ccttcggcac ttacccggca tgaaaagctg
     4261 ccgagttaat aattgaacgc gaatacgctg aacatctgcc gaataaacat tcactcccga
     4321 gccgatttaa tacagctcat cgcctcaaac ttttccgct
//
LOCUS       DAOQGS010000352         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10835_length_4350_cov_4.971828, whole genome shotgun sequence.
ACCESSION   DAOQGS010000352 DAOQGS010000000
VERSION     DAOQGS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10835_length_4350_cov_4.971828"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PKZ52_12730"
     CDS             complement(<1..178)
                     /locus_tag="PKZ52_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNG60681.1"
                     /translation="MDVSYLLDGLNDAQRQAVCAEPSNQLILAGAGSGKTRVLVHRIA
                     WLIQVLGVAPHQIMA"
     gene            complement(485..1684)
                     /locus_tag="PKZ52_12735"
     CDS             complement(485..1684)
                     /locus_tag="PKZ52_12735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60682.1"
                     /translation="MPQALSPSREQALLLTLMAAQFTNILDFMIMMPLSAQLMAVFDI
                     STGQFGLLISAYSLSAGATALVASAILDRYDRRRVLLLSYSGLILATAACALAPNHIM
                     MLVARCMAGLFGGVATSIVMAIVGDVIPLERRGRAMGVVMLAFSLASVAGVPAGIWIA
                     DHFSWQAPFICVAAVSCVVLLGCWVWVPSLTEHVAAKHENLLQSYRGLLSNTNHLWAF
                     ATSFLVMLAGFMTIPYIAPSLVSNAGLSHSQLPYIYLLGGALTLITRPLIGRITDRHR
                     HALVLNVLMAISLIPIVLVSQTLPLGLPYQLLVAGLFFVFVSGRFIPCSAMVTASCTP
                     QYRGRVMSFNSAVMNLGSGLAAMLAGYIMTSDEAGRLVHYDWVGYISGVITLVGMRVA
                     WRVRPVS"
     gene            complement(3276..3974)
                     /locus_tag="PKZ52_12740"
     CDS             complement(3276..3974)
                     /locus_tag="PKZ52_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HNG60683.1"
                     /translation="MNIHKAATRTKTLVALGALVFSSSAFCAPNLITNGSFEEPALPA
                     GSDYLIAPVGSAFIPGWSVVGIEGKDVVIVDSAFDLPDGSGVHFWAAGGSQWIDLTGA
                     GANTFEGVAQTLNLDPGLYSLGFSVGNIVTNIALGKQSTVSLWINGAQVSTFTNSAGN
                     STDVTWQPFTYTFTAAGLTTLEFYNGDSKRDTLSGLDEVFLTAITPVPEPSAASLILA
                     GLALMGFCCGKRRV"
BASE COUNT         1156 a         1056 c         1245 g          893 t
ORIGIN      
        1 ccgccatgat ttgatggggg gccacgccca acacctggat caaccaggcg atacggtgca
       61 ccaacacgcg ggttttaccg gagcccgccc cggcgagaat caactggttg ctaggctcgg
      121 cacagaccgc ctggcgctgg gcgtcgttta agccatcgag cagataagag acatccataa
      181 ccgaccactg ggtaaataaa caggggtggg attgtagcag tatcgtctgg ggataaaacc
      241 tatccagctg tgggcactgc acattggctg gtagttgggt ttaccgctgg gtgagtggtg
      301 agctttggtt atggtgtgtc agatgaagta gtttaggcat tattcggtta tttcttgggc
      361 cgccttgcct agccgacgcc taggtagtat tgaaaatcgt taaaacaccg cgcaaatctc
      421 cgaccatatc aactactaaa ccacaacacc gtacaccaaa taaccggctc atacatatct
      481 ggtgttaaga aactggccgc acccgccaag ccacccgcat ccccaccaga gtaatcacac
      541 cactgatata ccccacccaa tcgtaatgca ccaggcggcc cgcttcatca ctggtcataa
      601 tgtagcccgc cagcatggcg gcaaggccgg agcctaggtt catcaccgct gagttaaacg
      661 acattacccg gccccggtat tggggtgtac agctggcggt gaccatggcg gagcagggga
      721 taaagcggcc ggagacaaat acaaaaaata accccgccac cagcagttgg tagggtagcc
      781 ccaggggtag ggtttgggag acgagcacaa tgggaattag gctgatggcc ataagcacat
      841 ttaacaccag ggcgtggcgg tggcggtcgg tgatgcggcc gatcagtggg cgggtaatga
      901 gtgtgagggc gccgcccagc aggtagatat agggcagctg gctgtggctt aagcctgcgt
      961 tggaaactag ggagggcgct atgtagggaa tggtcataaa gcccgccagc atcaccaaaa
     1021 acgaggtggc aaaggcccag agatggttgg tgttgctcag taatcctcgg taactctgca
     1081 gcaggttctc atgcttggcc gctacgtgtt cggtaaggct tggcacccat acccagcagc
     1141 cgagcaaaac cacacaagaa acagctgcca cgcagataaa cggtgcctgc caggagaagt
     1201 gatcggcaat ccagatgcca gcgggcaccc ccgctacact ggctaaggag aacgccagca
     1261 tcactacacc catggcccgc ccgcgccgct ctaaggggat tacatcaccc actatggcca
     1321 tcacgatgga ggtggcaacc ccgccgaata atcccgccat acagcgcgcc accagcatca
     1381 ttatgtggtt gggggccagg gcgcaggcgg cagtggcgag gattaagcct gagtaactca
     1441 gcagtaacac ccgccgccgg tcgtagcggt cgaggatggc ggaggccacc agggcggtgg
     1501 cacctgcgct cagggaatag gcggagatca acaggccaaa ttggccggtg ctgatatcaa
     1561 acactgccat cagctgggct gacagaggca tcatgatcat aaaatccaga atattggtga
     1621 actgtgctgc catcaatgtg agcaacagag cctgttcgcg gctggggctt agagcctggg
     1681 gcatataaaa cctaggattg aggggcgtgt ttgggggtgc ttgaggtaga ggatagatgc
     1741 taaagcctgc gcctgggttt gtcagaaatt tcctctttaa attgcccgtc aaatacttgt
     1801 cggcccgcgc cagtgaaggc cttaaaacat gccctgtgac ggctctttgt catgtgcaag
     1861 ctgttgaaaa ataaagttat ttccattcat ctggcggctt ggccgcctgt cgcggcgggc
     1921 tggtctttgg gcttaaaagt ccgttttggt cgcaatgctg caaagcggtg atttctctag
     1981 ttcttcagtc aattgctgcc gccactatgg acaatttcgc acacgaagaa gcgtttgtgg
     2041 cgactaaacg cctctgtaat tttgaaccat actgtcttca agttgaggcg cgaaggaatg
     2101 acgtagccgc aaacggaagg aaagagttat taggttgtta agtggatgct aaaccggcac
     2161 ggactagccg caggggccga aaaagagcgg gggacggatt gtcagggaat tgaataatgg
     2221 aactggcagg agacatacgg gaaaacaaag gaattaaaga cggataggcg taaaggacga
     2281 atttaaggag tgcgaatgga agaggttggc ggtgctaact cataaacagg accggcccat
     2341 aaccaggcca gccaaaagga ttgttgaggg aatgcatata cagataaggg aaacagaaaa
     2401 ggaatacggc taaggatgca ggtttaagga ttagccccat tgggcaaagg actctaaagg
     2461 aactggagac gggataaaaa ttgggagtgc agcgaaggat tagagcgcaa gggacagtga
     2521 tatacaacag catggtaatg gataaagcgc ccaggcgcag tgcgtggaga ggaaccccgc
     2581 atcaaggagc acgagcacgt gcagtgaggg catacaggga gccgagaagg attggcgtta
     2641 tggattgcag caggatgagg gctgatggga ctgggtttaa atggagcacc gaaggatcaa
     2701 tcctgtggaa acaggtttaa ggacacataa gaaaccaagg acactaccta agggaattca
     2761 ccaaagggac gcgcttaggg agcacacagg aaccgcgtag gaatatgcag caggcactgg
     2821 ggattgggct gcacttcgca tagggacaca ctgggagcca gacaacagga acacacgggg
     2881 cccgctaggc tgtttgcttg ccgcagcgga acaattggca cctccccaac ggaatggcct
     2941 atcaagcaaa ctcataagct aagtggcgat acatgactga tccgattcgg ctggccctaa
     3001 atacacagta tttggggctt cgttttttta taaacatcag ttgagccgaa gcgaggacgc
     3061 cttagcaagc tgcaaagggt taaccgagtt caggtgacgg ttacatccag tgttactacc
     3121 cgcctaatga ttctcaccgc ccaaagtggg cgccctaaga atggtcagag cgacctaaga
     3181 ggtgccccac gcacaagaag acacccccta agttgaacaa ataaacgcct tgtcgtgttc
     3241 aggctcgagg cgcttgccga ctggactttt agccgctaaa ccctgcgttt gccgcaacaa
     3301 aaacccatca aggctaaacc ggcgagtatc agactcgcgg cagagggttc gggcactgga
     3361 gtaatggccg ttaagaacac ttcgtccaag ccactgaggg tatcgcgttt cgagtcgcca
     3421 ttataaaact caagggtggt gagccctgca gctgtaaatg tataagtaaa tggctgccaa
     3481 gtcacatcgg tactattgcc cgccgaattg gtaaacgtac tcacctgcgc gccgttaatc
     3541 cacagtgaca cggtgctttg tttgcctaag gcaatattgg taacgatgtt tccaaccgag
     3601 aagcccaggc tatataaacc gggatcaaga tttaatgtct gggccacacc ttcaaaggtg
     3661 ttggcgccag cgccggttag gtcgatccac tgtgacccgc ccgcagccca aaaatgcacg
     3721 ccggagccat cgggtaaatc aaaggcggaa tcaacaatta caacgtcttt gccttcaatt
     3781 ccaaccaccg accagccggg aataaatgca ctgcccactg gggcaattaa gtaatcggag
     3841 ccagcgggta gggccggttc ttcaaagctg ccgttggtaa ttaggttggg tgcgcaaaag
     3901 gctgaactag aaaacaccag tgcgcctaac gcgactagtg tcttggtgcg tgttgctgct
     3961 ttatggatat tcattgcttc cgcacagcta gtaattgatt gctatttttg tgtgcatagc
     4021 cccgcctgca taaattggtg taaacctaaa gtgggcagat ggattaacgc ttcgggataa
     4081 tgaatcgaaa tccggctgaa cgcagggtgg ccactcaata tactgtcttt gctcggcgtg
     4141 tagacggttg aattacatgg tatgaaagtg tcacggagag agatcttgcc caaatgcgcc
     4201 gactttatcg gctcgctcca gcgcggctgt ttaaataaac tctaaccttg cgcatgcggg
     4261 tgatacggtt gccggtaatt tactgaaaaa actttgtaac tattcagcat cttttaatgt
     4321 ggctggaggt aattcctgca ctgactagga
//
LOCUS       DAOQGS010000353         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10885_length_4337_cov_4.596217, whole genome shotgun sequence.
ACCESSION   DAOQGS010000353 DAOQGS010000000
VERSION     DAOQGS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10885_length_4337_cov_4.596217"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKZ52_12745"
     CDS             complement(<1..756)
                     /locus_tag="PKZ52_12745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNG60684.1"
                     /translation="MGKNRIFLENNNGILQGSITPANSLLILLNKGVFAVMFAAKNER
                     DISIFIIAIVAILFFHGINLDGLPVGLHSDESSIGINAAAIAKNGTDEHGNSFPIYFK
                     AFGEYKNPIYIYMCGLLFKVFGVSAQALRTTSCLFFVLGFIHCLMLIRNLFPKNRTVL
                     FFAALNFGFLPVLFVLSRISFEVISQFTFTAWILYVTWQIIEYSNNDKKRQLTALALG
                     LLIGLSVYSYSTARLLSALYCFSLAIIFCNRRDF"
     gene            complement(959..2569)
                     /locus_tag="PKZ52_12750"
     CDS             complement(959..2569)
                     /locus_tag="PKZ52_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60685.1"
                     /translation="MKFFIPPQLLAALSFLQSDKCNDYAYRAVFIFFSLFLILPLWTI
                     TYLPLGDLPDHAGQLNVIMHYSEYANEYRINWFTPYLVGYSISLFFGIFFSATTALKI
                     TYSLAVLAVPIATASLLRNLQLNRYWVIPSFACAFSFSFYWGFFSYVVATPIAIAFYA
                     FCVNYGRTSQNIKTAVIAALFSALLFFAHAMAWAFSMMVAACILFIYNNLKETKRKLL
                     PFIALLPLVAYWVSINGATQSQPIESGHYIEHITSKLGNEINYIIEQFKDRTAKHEHT
                     QRAKELLSFSIGRMPLLDFVALALILVFWPTLIGARLTRNWRRWLPPLCAVGAFMVVP
                     YWIFDTAYVNLRFAAFLIPLSVFLFEVNKTQSPVEQYGPVSFAARFLLGLSITYVLLA
                     GNYRTIASFKSNDDDFKTILSKMEPNKIVLALMFDQDSALTFAPPYMHFGSYYQAEKG
                     GVVIASFSHDPVAHNVPVRYQGAPWPLPDTWDPSKFNWQQHHGDQYDYFLVRSKTLRD
                     NLFSAVTGKVTLEAKQGDWLLYKNDKSL"
     gene            complement(2584..>4337)
                     /locus_tag="PKZ52_12755"
     CDS             complement(2584..>4337)
                     /locus_tag="PKZ52_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60686.1"
                     /translation="FQQAARTYPSIYINPHLPRSPAGKFFPEGSRAILPTKICLIVEL
                     GDIQTLYTVPNRQGQKLSSLHKLNVRHRKPGHQPSLLYGNNNVRSQRQLGEYSHKPSK
                     LLFSRAQSPNCLKKEQHLNSVPWFCGRTSFPLESPPLNTVWAQAHTFMHRCQMSELLK
                     GCDESLPKVSNGYIGMSGVQISLFLATILVFTFTILLSLPHETADEGFHTPQIWEFFS
                     GNYKIEASLTVLPVYHAIIAGVLKAVGHFSIPFARFVHLCIACLALPVFWQICHVLRH
                     QDSDTRILQFLLFPIILPFFSLIYTDIPALLFVLLMVLYTLKKNYWAAAALALVAVSM
                     RQTNLIWMGFCGAIIIGEHWPHLFNRRWDSFKHEFIPLFLKLLPYGLTTLAAGCFFYF
                     NGGVAVGDSHQHQISINLSNVYFFLLLSFVFFLPYNLAYTQKIFDILKGKPKVWYGIA
                     MCFVVYMATYSNSHQYNSFGLSFYLRNVVLHYTVEFPAAKALTFLPICWSALTFYVLA
                     NEGKVKWRIWVLYAFIIISVLPLPLVEQRYYIVAFVMFLAMKPSTHPLADRFCLFIYL
                     PISATLLYFISQAKLFL"
BASE COUNT         1316 a          841 c         1057 g         1123 t
ORIGIN      
        1 aaaatctcgc cgattacaaa agattattgc aaggctaaaa cagtagagtg ctgacaataa
       61 tctagcggtt gagtaggagt agacagacag ccctatgagt aagcctaagg ctagagctgt
      121 tagttgccgt ttcttatcgt tgttgctgta ttcaataatt tgccatgtaa cgtaaagtat
      181 ccaggcagtg aaggtgaatt gggatataac ttcgaaggaa atgcgtgaaa gtacaaacag
      241 aacaggcagg aaaccaaaat tcaatgctgc aaaaaacaaa acagttctat ttttgggaaa
      301 taagtttcga ataagcatta ggcagtgtat aaagcctaat acgaaaaata aacagctggt
      361 tgtgcgtaaa gcctgggcgc taacgccgaa gaccttaaac aaaaggccgc acatgtatat
      421 atagatagga ttcttatact ctccaaacgc tttgaagtaa ataggaaagc tattgccgtg
      481 ttcatcagtg ccgtttttgg cgatggctgc ggcatttatg cctatagagg actcatctga
      541 atgcagccct accgggagtc cgtctaggtt aatgccatga aaaaacagta tcgcaactat
      601 agcaattata aaaatgctaa tgtctctttc gttcttggct gcaaacataa ctgcaaaaac
      661 tcctttgttc aaaagaatta acaatgagtt cgcaggggtg atgcttcctt ggagaatccc
      721 attattgttt tctaaaaaaa tcctgttctt acccatcctc agccgaaatg taaacggcta
      781 tgtatattga gcaggaattt ttatggagtg gtaagtgcca cacctaaatt aatggtatat
      841 acgttatatc agtgtgcggt atctcaatgt gagttgagcc tcaatttgga aatcacattt
      901 cactttggct gcgccctgtt catgaacatt ctagaaaaac gcattagggc taaggttttt
      961 aaagggattt atcgttcttg tacagcagcc aatcaccttg ttttgcttca agagttacct
     1021 tgcctgtaac cgctgaaaag agattatctc gaagtgtttt cgagcgcact agaaaatagt
     1081 catattgatc gccatggtgt tgttgccaat taaacttgct aggatcccag gtgtcgggta
     1141 ggggccaggg ggcgccctgg taacgtacag gcacattatg tgccacagga tcgtgagaga
     1201 agctggcgat tacaactccg cctttttccg cttgataata gctaccaaaa tgcatataag
     1261 gcggcgcaaa cgttagggcc gaatcctgat cgaacattag ggccaataca attttgtttg
     1321 gctccatttt gctcagaatg gttttgaaat cgtcatcatt agatttaaaa cttgcgattg
     1381 ttcggtaatt tcctgccaaa agaacgtaag taatagaaag ccctaataga aagcgcgcag
     1441 cgaaagagac tgggccatat tgttcaacgg ggctttgggt tttatttact tcaaataaaa
     1501 acacgctcag agggattaaa aaggccgcaa agcgcaggtt tacgtaagct gtgtcaaaaa
     1561 tccagtatgg aaccaccata aacgcaccga cggcacaaag cgggggcagc catctcctcc
     1621 aatttcgcgt cagcctggcg cctatgaggg ttggccagaa aactagtatc agggcaagag
     1681 ccacgaaatc caaaagaggc attcggccta ttgaaaagct caacaattct ttggctctct
     1741 gggtgtgttc gtgttttgct gtgcggtctt taaattgttc aatgatatag tttatttcat
     1801 tgccaagttt gcttgttata tgctcgatat aatgcccgct ctcgatgggt tgcgactggg
     1861 tcgctccatt gatggaaacc caatatgcaa ctagaggcag cagcgcaata aatggcaata
     1921 atttacgttt ggtttcttta agattgttgt aaataaaaag aatgcaggcc gctaccatca
     1981 tggagaatgc ccaagccatg gcgtgagcga aaaacagcag tgctgaaaat agggctgcaa
     2041 tcacagcggt ttttatattt tggctcgttc ggccataatt gacgcagaaa gcatagaagg
     2101 caattgctat cggggtggct actacgtagc taaaaaaccc ccaataaaac gagaagctaa
     2161 aagcgcaggc aaagcttggg ataacccagt atcggttgag ttgtaaattg cgcagaaggc
     2221 ttgcagttgc aatggggact gcgagcaccg ccaaagagta agtgattttc aacgccgttg
     2281 tggcggagaa gaaaatgcca aaaaatagac taatgctgta gccaaccaaa tagggagtaa
     2341 accagttgat acgatattca ttggcgtatt ccgagtagtg cataattaca tttaactgcc
     2401 ctgcatgatc cggtagatcg cccagtggta aataggtgat agtccaaaga gggagtatta
     2461 gaaataggct aaagaatatg aatactgctc tgtacgcata atcgttgcat ttgtcagatt
     2521 gtagaaaact caaggcggcc agcagctggg ggggtataaa gaacttcatg agctggctga
     2581 tccttataaa aataatttgg cttgcgagat aaaataaagc agcgtagcgg agataggtag
     2641 gtagataaag aggcagaacc tgtcagccaa cgggtgcgtg ctgggtttca ttgcgaggaa
     2701 cattacaaac gcaactatgt aatagcgctg ctccactaag ggtaagggca atactgagat
     2761 aatgataaat gcatagagca cccagattcg ccatttaacc ttgccttcgt tagccaaaac
     2821 atagaaggtt aatgcgctcc agcagatagg caggaatgta agggctttgg cagcggggaa
     2881 ttccaccgtg tagtgtaaaa ccacattacg caaataaaag gaaagcccaa aggaattgta
     2941 ttggtgacta ttggagtatg tagccatgta taccacaaag cacatggcaa tgccgtacca
     3001 aactttgggt tttcctttga gaatgtcgaa tattttttgc gtgtaagcaa ggttgtaggg
     3061 cagaaaaaat acgaaactta gtagtaggaa aaagtagacg ttggataggt tgatggagat
     3121 ttgatgttgg tgggaatcgc ccaccgctac accgccgtta aaatagaaga agcatccagc
     3181 cgctagggtc gttaatccat acggtagtag ttttaaaaac aagggtataa attcgtgttt
     3241 gaaagaatcc cagcgtcgat taaacaaatg aggccagtgc tcaccaatga tgattgctcc
     3301 gcaaaaaccc atccatatca aattagtttg ccgcattgaa accgcaacca atgcgagggc
     3361 tgccgccgcc cagtagtttt ttttcaaggt gtacagcacc attagaagca caaacaaaag
     3421 cgcggggata tcggtgtaaa taaggctaaa aaatggcagg attatgggga ataacaaaaa
     3481 ttgaagtatg cgggtatcgc tatcctggtg gcgcagaaca tgacatattt gccaaaacac
     3541 cggcagtgcc aggcacgcga tacacaggtg gacaaagcgc gcaaatggaa tagaaaaatg
     3601 ccccacggct ttgagtacac cagcaataat ggcgtggtaa accggtagca ctgtgagtga
     3661 ggcttctatt ttgtaattac cgctaaagaa ctcccagatt tgcggtgtat gaaaaccctc
     3721 gtcagcggtt tcatggggga ggctgagcaa tatagtgaag gtgaagacca gaatcgttgc
     3781 taggaatagg gagatttgga caccactcat tccaatataa ccatttgata ctttaggaag
     3841 actttcatcg catcctttaa gtaattctga catctgacac ctatgcataa acgtgtgggc
     3901 ttgtgcccag actgtattta agggcgggga ttctagagga aacgaagttc tgccgcaaaa
     3961 ccaaggcacc gaattgaggt gttgttcctt ttttaagcag tttggtgatt gggcgcggct
     4021 gaacagcagc ttagatggct tgtgtgagta ctcacctaat tggcgttgtg atctcacgtt
     4081 gttattcccg tataacaatg acggctgatg gcccggtttg cggtggcgta cgttcagttt
     4141 gtgcaagctt gatagctttt ggccctgtcg atttgggact gtatataggg tttgtatatc
     4201 gcctaactcc acaattaagc aaatttttgt agggagtatt gcccgactac cttctggaaa
     4261 aaacttacct gctggagaac gaggtaaatg cgggttgata taaattgatg ggtaagtcct
     4321 agccgcctgt tgaaata
//
LOCUS       DAOQGS010000354         4336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_10890_length_4336_cov_4.623686, whole genome shotgun sequence.
ACCESSION   DAOQGS010000354 DAOQGS010000000
VERSION     DAOQGS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10890_length_4336_cov_4.623686"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKZ52_12760"
     CDS             <1..487
                     /locus_tag="PKZ52_12760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG60687.1"
                     /translation="WQHLPDTPQFDAIYTDEVMVHIPDLTAFFNKCKRMLKPGGRMVH
                     KDLHFTHSQFKHAKDPLSQHVNKMYAYSGHYRTLVDMLSALDAAGFHLDQIKQIPIEH
                     YKRTMDQVWIPNINNNRRYLEQLTSPKHTREFKLYLKAILLLFRQKVFNLHIVSAAKA
                     D"
     gene            509..1336
                     /locus_tag="PKZ52_12765"
     CDS             509..1336
                     /locus_tag="PKZ52_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60688.1"
                     /translation="MNHTYETAGYLIEQFLARGQLHESSVTLGGTGPNGTSQLALLEL
                     IGFDKVRALKAINLISGSVFGYFIYLAFKERKMHVEHYLNYDKGARDLHGASLIKGAS
                     FFCGFKRGRSSLYDNHLVEATVRYLIHDDFCERPLRSFDGNMVFWAYCGRRQALIKIT
                     PETFPDMNVWQVITACLSIHFIHGEYAYGDYQFSDPMFSPQFKALVRKLLRSGDNHLF
                     INYKKTQVAGNIIFIKSQPCRFPNWVLLRDFLTFTCNMKNPRLIKTHRHNVEMLLRG"
     gene            complement(1450..3312)
                     /locus_tag="PKZ52_12770"
     CDS             complement(1450..3312)
                     /locus_tag="PKZ52_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG60689.1"
                     /translation="MPEYKKIVDKIWSAKAEAFETEHEPWIYANRLNIFIGPNNSGKS
                     LFNRALISKEFQWVDETVPNKLLKMLELYKIHISQHPRSRLISPAPTPPGADIIQELK
                     NAIENLSRPVTKDQFDTTKFLLDQHTKFLLKEINRDTSFSGPPYRELYNSIINEPQDI
                     FKIPMRQEQSWHYIPILRGLRPLTNSLDNHYSMRTQKDYSTDCNTHTGLELYDLITSH
                     LLGEPEEREKVRNYEKMLSENFFNYAEVSLTPKIRTDTLHAKIGHEKQRPIYQLGDGL
                     QTVIIITAAAFFTEKPSLFAIEEPETHLHPGFQRRLIEVLLDKTPHQYFVTTHSNHLL
                     ELAECRDEISIFRVNKEPGEQTKFKISVCDQDRDLLRELGVNPASVYLANCTIWVEGI
                     TDRLYIQAFLEQYKKHNPGQYDHWIENYHYTFVEYQGAGLAHWEFADEQNHDQEQMLA
                     TITTPNILVIADGDIETKGNRQGKLESQLAEKLYITPGKEIDNLVPTEVIKSFVKARI
                     DGMIKDNKENKAKIQRHNIKPENIDRLHNLSNEQKGIGWHIDKLLFGRDPTEDEYRLF
                     SKLGSATGTIRQKVELCRYATAKMRAEEWEMSEEIKKLCEIIFEHIAENNTTGR"
     gene            complement(3593..4123)
                     /locus_tag="PKZ52_12775"
     CDS             complement(3593..4123)
                     /locus_tag="PKZ52_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNG60690.1"
                     /translation="MGRSRYHFTHPDQPHFLTLTVLHWIPIFTRPATVQILFDSFQFL
                     INQGLTLYAYVILENHLHLVAQSPQLNKDIARFKSFTARALIAYLQEHNVKTILDQLA
                     FYKKAHKSDRPLQFWQEGTHPELIQNEAMMRQKIEYIHHNPVKRGYVDMAEHWRYSSA
                     RNYAGREGLIPVNKAW"
BASE COUNT          994 a          870 c         1052 g         1420 t
ORIGIN      
        1 ctggcaacat ttgcccgata caccgcagtt tgatgctatt tatactgatg aagtgatggt
       61 gcacataccc gatttaactg cattttttaa caagtgcaag cgtatgctaa agcccggcgg
      121 tcgtatggtg cacaaggatt tgcattttac gcattcgcaa tttaaacacg ccaaagatcc
      181 gctgagtcag cacgtcaaca aaatgtacgc ctacagcggc cattaccgca cactggttga
      241 tatgttgtca gcattagatg ctgcaggctt tcatttagac caaataaaac aaatccctat
      301 tgagcactat aaacgcacca tggatcaggt gtggattcct aatatcaata ataataggcg
      361 ctatttggaa caattgacca gccctaagca cacgcgggaa tttaaactgt atttaaaagc
      421 tattttgctg ctatttcggc agaaggtatt taatctgcat attgtatctg cagcaaaggc
      481 cgattaattg ccagcaagga gcaatgctat gaatcacact tatgaaacgg cgggctattt
      541 gatcgagcaa tttcttgccc gtgggcagct gcatgaatca agtgtgacct taggtggaac
      601 cggcccaaat ggcacaagtc agttggcgct tctagagctg attggttttg acaaggttcg
      661 cgcgctcaag gccatcaatc taatttccgg cagtgtattt ggatatttta tttatttggc
      721 ctttaaagag cgcaaaatgc atgtggagca ctatcttaat tatgataagg gcgcgcgtga
      781 cttgcatgga gcttctttaa tcaagggcgc cagttttttt tgtgggttca aacgaggccg
      841 ctccagcctg tacgacaatc atctggttga ggcaacagtt cgctatttaa ttcacgatga
      901 tttttgtgag cgcccattgc gctcttttga tggcaatatg gttttttggg cctattgcgg
      961 gcgcaggcag gcgcttatta aaatcacccc tgaaaccttt cccgatatga acgtatggca
     1021 ggttattact gcctgtttgt ctattcactt tatccatggt gaatatgcct acggcgatta
     1081 ccaattctcg gacccaatgt tttccccgca gtttaaagcg ctagtgcgta aactgttgcg
     1141 cagtggggat aatcatcttt ttattaacta caaaaagacc caggtcgcgg gcaatatcat
     1201 cttcatcaaa agccaacctt gccgatttcc taattgggtg ctgttgcggg attttttaac
     1261 ttttacctgt aatatgaaaa acccgcggtt gattaaaacc catcggcata atgtggagat
     1321 gttgttgcgg ggttgatgca catatttcgc agggtgggca agcttgcttg cccacccttg
     1381 gtggccgaga ttttatcgac ggttgggatt ttattgtgtt atcgattggc gggcaaagag
     1441 ttgctcaccc tatctaccag tcgtattgtt ttctgctatg tgctcgaaaa ttatttcaca
     1501 gagtttttta atttcctcac tcatctccca ttcttccgct cgcatcttgg cggttgcgta
     1561 tcggcaaagc tctactttct gcctgattgt gcctgtggct gagcctaatt ttgagaagag
     1621 gcgatattca tcctctgtag ggtctctgcc aaagagaagt ttatcaatat gccagccaat
     1681 acctttttgt tcgtttgata ggttgtgaag tcgatcgata ttttctggtt ttatattgtg
     1741 tctttggatc tttgccttat tttccttgtt atctttgatc atgccgtcaa ttcgtgcttt
     1801 tacaaatgat tttataactt cagtcgggac taagttgtca atttcttttc ctggtgtgat
     1861 atagagtttt tctgctaatt gactttctag ctttccctgc ctattacctt tggtttcaat
     1921 gtctccgtca gcaataacga gaatattggg cgtggtaatt gttgcgagca tttgttcttg
     1981 gtcatgattc tgctcgtccg caaattccca atgggctaag ccagctccct ggtattctac
     2041 aaacgtgtaa tgatagtttt ctatccaatg gtcgtattgc ccggggttgt gttttttgta
     2101 ttgttctaag aatgcttgta tatataagcg atcagttatt ccctcaaccc atattgtaca
     2161 gtttgccagg tacaccgacg ctgggtttac accgagttcc ctcaataggt cacggtcctg
     2221 gtcgcaaaca ctgatcttaa atttagtctg ctcgcccggt tctttattga cgcggaagat
     2281 actgatttca tcgcggcact ctgctagttc caagaggtga tttgagtgtg tggtgacaaa
     2341 atattgatga ggtgtcttgt cgagcaaaac ctctataagt cttctctgga agccagggtg
     2401 caaatgtgtc tctggttctt ctattgcgaa tagcgacggt ttttcggtaa aaaatgcagc
     2461 agcagtaatt ataatgacag tttgtagacc gtcgcccaat tggtaaatgg gtctttgttt
     2521 ttcatggccg atttttgcgt gcaaggtatc ggttctgatt ttaggtgtta atgatacttc
     2581 agcataattg aagaagtttt cggatagcat tttttcgtaa tttcgcactt tttcgcgttc
     2641 ttctggctcc ccaagtaagt gggaagtgat taagtcatat aattctagtc ccgtatgtgt
     2701 attgcagtcg gttgagtaat ctttttgggt gcgcatactg tagtgattgt ccaatgagtt
     2761 ggttagtggt cttaatcctc ttaaaatagg tatgtagtgc cagctttgct cctggcgcat
     2821 agggatttta aaaatgtctt gaggttcgtt tattatcgaa ttatatagct cacgataggg
     2881 cggaccgcta aagctagtat ctcggttaat ttcttttagg agaaacttgg tgtgttgatc
     2941 gagcagaaat tttgtagtgt cgaattgatc ttttgtgaca ggtcttgata aattttcaat
     3001 cgcgtttttt agttcctgta ttatatctgc gcctggaggg gtgggtgctg gtgagattaa
     3061 tcggctgcga ggatgttgag aaatgtgtat tttatatagc tctaacattt ttagtagctt
     3121 gtttggaact gtttcgtcaa cccattgaaa ctctttggat attagcgcgc gattaaacaa
     3181 gctcttccct gagttgtttg gcccaataaa aatattgagc cggtttgcgt aaatccaggg
     3241 ctcgtgttct gtttcaaaag cttcggcttt tgcagaccaa atcttatcga caatcttctt
     3301 atattcaggc attggctcct ccagtgaaaa gcacatttaa gttcaaattt ttattggcta
     3361 ttttgcggtg ctgcttggca gttcaccgag tttctgtttg ttcagcgttt tctgctcgtt
     3421 ccgccaaggc gcattctggc ctatttagtc tctatgcatt cccacgcgga gcgtgggaac
     3481 gaggagaaca tcaaagaggc agttgtatgt gttccggtgc tgctatgccc tgtgcgggag
     3541 taggagcgtc catcaccatt ctcgttccca cgctccgcgt gggaatgcat acttaccagg
     3601 ctttatttac cggaattaag ccttcccgcc cagcgtaatt tctggcgcta gaataacgcc
     3661 aatgctccgc catatccaca taaccgcgtt taaccgggtt gtggtgaatg tattcaattt
     3721 tctgtcgcat catggcttca ttttgaatta attctgggtg tgtgccttct tgccagaatt
     3781 gtaatgggcg gtctgatttg tgggcttttt tgtagaaggc gagttggtcg aggatagttt
     3841 tgacgttgtg ttcttgcagg tacgcaatta atgcgcgggc ggtgaaggat ttaaagcggg
     3901 ctatgtcttt gtttaattgc gggctttggg cgactaagtg aagatgattt tctagaatga
     3961 cgtaggcgta gagagttaag ccttgattga ttagaaattg gaatgagtcg aagaggattt
     4021 gtaccgttgc gggccgggta aatatgggaa tccagtgcag gacggtcagg gtgaggaagt
     4081 ggggttggtc gggatgtgtg aaatggtaac ggcttctgcc catgggttag tccttttagg
     4141 gtgagtttgt tatgcgttcc tggagtgcgg tgtttatgtt tagttttggt ctgcattccc
     4201 acgcggagcg tgggaacgag gtgttgcttg ggggggaagt gggttgggaa cttacttcgc
     4261 cccagccccc ttaatcgcct gtaaatagtc cccaccattg cgatgctgcg caatcacctg
     4321 cgccatggtt aaaccc
//
LOCUS       DAOQGS010000355         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11041_length_4294_cov_4.494692, whole genome shotgun sequence.
ACCESSION   DAOQGS010000355 DAOQGS010000000
VERSION     DAOQGS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11041_length_4294_cov_4.494692"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKZ52_12780"
     CDS             complement(<1..1106)
                     /locus_tag="PKZ52_12780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNG60691.1"
                     /translation="MNKTIEAVTARIVARSKPRRDAYVAAMKATMDNNPPKKRLSCGN
                     LAHAYAACGSQDKDTLRLLQSANISITTAFNDMLSAHQPFESYPNIIKAAARAMGSTA
                     QVAGGVPAMCDGVTQGQPGMEMSLFSREVVAMACAVGLSHNMFDGNMFLGVCDKIVPG
                     MVIGALSFGHIPAVFVPAGPMHSGISNKEKAKVRQQYAAGEVGEDKLLEVETKSYHSP
                     GTCTFYGTANSNQMLVEMLGVQLPGASFVSPDSPLREALTRAAVEKLILSTQAAGTYR
                     PLYDVITEYSMVNAVVGLLATGGSTNNTLHLVAMARAAGIELTWEDMDELSRATPLLA
                     RIYPNGEGDVNQFHAAGGMSYLVHELRRGGLLNE"
     gene            complement(1118..2572)
                     /gene="zwf"
                     /locus_tag="PKZ52_12785"
     CDS             complement(1118..2572)
                     /gene="zwf"
                     /locus_tag="PKZ52_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNG60692.1"
                     /translation="MKNTDILIVGGDGDLALRKLYPALYYLKLNNCMPSNTRIFGMAR
                     TPSEQSAFLAKVKEWLINYSAANLFNEETWLAFSENLTFVQGDSTKPDDLTRIKNTYF
                     GDDHELIIYLAVPPLIFGKIATSLDACGLAKPSTRIVVEKPLGDSKESFMAINAELAR
                     VFTEKQLFRIDHYLGKESVQNLLALRFANDIFEPIWNSKHIDNIQITVTETVGCGNRW
                     DFYDETGAMRDMIQNHLLQVLCLVAMEPPADLDADSVRGEKLKVLRCLKPITAENITQ
                     QTVRGQYAAGEIEGKAVPGYLEEEEGGRESNTETFVALKAEIANWRWSGTPIYIRTGK
                     RLSRRHSEIVIEFKQNNSKLFAKQLTENCANQLIIHLQPDEGIHIRMKNKRAGLDTSM
                     QLEDRYMDLSFSDNAKAKKHDAYTRMFYDVIRNDQTLFVSAGEVEAAWEWVDQIFAGW
                     KSQNQAVVPYTSGSNGPDAAFELLAKDGRRWIDG"
     gene            3327..>4294
                     /gene="gap"
                     /locus_tag="PKZ52_12790"
     CDS             3327..>4294
                     /gene="gap"
                     /locus_tag="PKZ52_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNG60693.1"
                     /translation="MPRIAINGFGRIGRNVLRALYERNLHDSIQIVAINDLGSPAINA
                     HLLKYDTVHGTFGFDVSHTNDAISVNGDSIKIIAERNPAALPWGELGVDIVFECTGLF
                     TAREKAALHLDAGAKTVLISAPGTNADLTVVYGVNHDKITPEHTILSNASCTTNCLAP
                     VAAALHDGIGIVSGLVNTVHSFTNDQNITDAYHSDMYRARAAGHSIIPTKTGAAAAIG
                     LVIPALAGKLDGLAVRVPTINVSLLDLTFQSARPTTVEEVNAVVAAAAASRFEGVLQI
                     NKLPLVSHDFNHSSASSVFDATQTRVIGGTQVKVLAWYDNEWGFSNR"
BASE COUNT         1091 a         1140 c         1009 g         1054 t
ORIGIN      
        1 ttttcattta ataaaccacc gcgacgcaat tcatgcacca ggtagctcat accgcccgcg
       61 gcgtggaact ggtttacatc gccctcacca ttgggataaa tacgcgcaag cagaggtgtg
      121 gcgcgggata attcatccat gtcctcccag gttaattcaa tgcctgcggc gcgggccatg
      181 gcgactaaat gcagcgtatt attggtagaa ccgccagtgg ctaataatcc caccacagca
      241 ttcaccatgg aatattcagt aattacatcg taaagggggc gataagtacc cgccgcttgc
      301 gtagataaaa tgagtttttc caccgccgcg cgggttaagg cttcacgtaa aggagaatcg
      361 gggctaacaa aagaagcacc cggcaattgc acgcctaaca tttccaccag catttggttg
      421 ctattggcgg tgccgtagaa agtacaggta cctgggctgt ggtaactttt agtttccact
      481 tccaataatt tatcttcgcc cacttcaccg gcggcatatt gctggcgcac cttggctttt
      541 tctttattgg aaatacccga atgcatgggg cctgcgggca caaacacggc ggggatatgg
      601 ccaaaactta aagcgccaat caccattccg ggcacaattt tatcgcacac acccaaaaac
      661 atattgccgt cgaacatgtt gtggcttaag cccacagcgc aggccatggc caccacctca
      721 cggctaaata agctcatctc catgcccggc tggccttggg ttacaccgtc acacatggcg
      781 ggcacgccgc ctgcaacctg ggcggtggag cccatggccc gcgcggccgc tttgataata
      841 ttcgggtagg attcaaatgg ctggtgcgcc gataacatat cgttaaacgc ggtggtaata
      901 ctgatattgg cgctctgcaa taaacgcaga gtgtctttat cctggctgcc acaggcggca
      961 taggcgtggg ccaaattgcc gcaggataag cgttttttgg gcggattatt gtccatggtg
     1021 gccttcatag cggccacata agcatcccgt cggggcttgc tgcgtgcaac gatacgcgca
     1081 gttacagctt caattgtttt attcacacaa cctccgatta accgtcaatc caacggcggc
     1141 catctttggc caagagttca aatgcggcat cagggccatt cgaacctgat gtatagggca
     1201 ctacggcttg gttttgcgat ttccaaccgg caaaaatttg atccacccac tcccaggccg
     1261 cttctacttc accggcggaa acaaataggg tttgatcgtt gcgaatcacg tcgtaaaaca
     1321 tacgcgtata agcatcgtgt tttttcgcct tggcattatc cgagaaggat aagtccatgt
     1381 agcgatcttc caattgcata ctggtatcaa gacccgcacg tttatttttc atgcgaatgt
     1441 gaataccttc atcgggctgc aaatggataa tcaactggtt ggcgcaattc tcagttaatt
     1501 gcttggcaaa taatttggag ttgttttgtt taaattcgat aacgatttct gaatgcctgc
     1561 ggcttaaacg cttgccggtg cggatataaa taggggtgcc gctccagcgc cagttggcga
     1621 tttccgcttt taaagccaca aaggtttcgg tattactttc gcggccaccc tcttcctctt
     1681 ctaaataacc gggcaccgct ttgccttcga tttcaccggc ggcgtattgc ccgcgcacgg
     1741 tttgttgagt gatgttttca gcagtgatcg gttttaaaca gcgcaacact tttaactttt
     1801 caccgcgcac actatcggca tccaaatcag cgggcggctc catggccacc aaacacaaca
     1861 cctgcaaaag gtggttctga atcatatcgc gcatagcgcc ggtttcgtcg taaaaatccc
     1921 agcggttgcc gcagccgacg gtttcggtaa cagtaatttg aatattgtcg atatgtttag
     1981 aattccaaat cggctcgaaa atatcattgg caaagcgcag cgccaatagg ttttgcacag
     2041 attctttgcc caggtagtga tcgatacgga acaattgttt ctcggtaaat acccgcgcca
     2101 gttcggcatt aatcgccata aatgattctt tagaatcacc cagtggcttt tcaaccacaa
     2161 tgcgggtgga gggtttagcc aagccgcagg catcaaggga ggtggcgatt ttgccaaaaa
     2221 tcagaggcgg caccgccagg taaataatca gttcgtggtc gtcgccaaaa taggtgtttt
     2281 taatgcgcgt taaatcatcc ggcttggtag agtcgccctg gacaaaggtg agattttcac
     2341 taaaggccag ccaggtttct tcattgaata aattagctgc gctgtagtta atcagccatt
     2401 ccttcacctt agccaaaaat gcactctgct cgctgggcgt gcgcgccata ccaaaaatac
     2461 gggtgttaga aggcatacag ttattcaatt ttaagtaata cagcgcggga tagagtttgc
     2521 gcaatgccaa atcgccatca ccgccgacga tcaaaatatc tgtatttttc atgctaacac
     2581 ctacctaaat ttaagaaagg atttaaggca aaacgccctc atattgccgc cgcattaatt
     2641 caacagcgaa gagaagctca caccgtaaaa ttgtgtacca caagagcccc tgtttagact
     2701 ggaggtgtga actttttgca gctttcgcag gcagaaatct gcaggggcgc aacagtgacg
     2761 taccggcgcg cgtgcaacat attcacgcat tcagacttaa tcagagcatc ctataaaaat
     2821 gtgtatgcgc ccaaattggc tgtggacacg ggcaaacact ttagaaatta ctgtccacct
     2881 aaggacaaag gccaaggcgt ctagacaagc gtgaataacg ctaaagcggc aacaccaaca
     2941 gcgcatttaa aaccttgcgc accctaaaaa gtaccagcct attgcaaagt ttcagccagg
     3001 cgtattattc cttcgcacct gcgcccactg cttcagtgcc taatttgaca attcagtcgg
     3061 tggccaaacg ccacggcgac cgagcaagct tccgaataag agcgattgag cgcgtgcaac
     3121 ggttaagacc ggggcattgt agaggttggc cggttgcttt ttcaactgcg attacccata
     3181 atccacggcc tcttgaaccg gtaatgcacc cccgcaaagg tgtgcctaca tcgctaggta
     3241 tgccataaaa accggaacct catttaaagc aattcggtgc acagtctgct gtgtataccc
     3301 ctcacttgac caagcaggaa tttaaaatgc ctcgtatagc gattaacggt tttggacgca
     3361 ttggccgcaa cgtattgcgc gcactgtatg aaagaaactt gcacgattct attcaaatcg
     3421 tcgccatcaa cgacctaggt tcacccgcaa ttaacgccca tctgctcaaa tacgacacag
     3481 tgcacggcac ctttggtttt gacgtgagcc acactaacga tgccatctcc gtcaacggcg
     3541 atagcatcaa aattatcgca gagcgaaacc ctgcggcact gccttggggc gaactgggcg
     3601 tggacatcgt atttgagtgc accggcctat tcaccgcccg tgaaaaggcc gccctgcatt
     3661 tagacgcggg cgccaaaacc gtattaattt cagcgcctgg caccaatgcc gatttaaccg
     3721 tggtctacgg cgttaaccac gacaaaatca ccccagagca cacaatcctc tccaacgcat
     3781 cctgcaccac caactgtttg gcacccgttg ccgccgcgtt gcatgatggc ataggcattg
     3841 tctctggttt ggtcaatacg gtgcactcct ttaccaacga ccaaaacatc acagatgcct
     3901 atcactctga tatgtaccga gctcgcgccg ctggtcactc catcattcca accaaaaccg
     3961 gcgctgcagc cgccattggc ttggtgatcc ccgcccttgc aggcaagtta gacggcttag
     4021 ccgtgcgtgt tcccactatt aacgtttcgc tgctggattt aaccttccaa tcagcgcgcc
     4081 ccacgactgt ggaagaagtt aacgctgtag tagccgcagc cgccgcatcg cgctttgaag
     4141 gtgttttgca aattaataaa ttgcctttgg tatcccacga ttttaatcac tcatcagctt
     4201 cgtccgtgtt cgatgccacg caaacacgcg ttatcggcgg tactcaagtt aaggtgcttg
     4261 cttggtacga taatgagtgg ggattttcta accg
//
LOCUS       DAOQGS010000356         4292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11055_length_4292_cov_3.221383, whole genome shotgun sequence.
ACCESSION   DAOQGS010000356 DAOQGS010000000
VERSION     DAOQGS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11055_length_4292_cov_3.221383"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKZ52_12795"
     CDS             complement(<1..943)
                     /locus_tag="PKZ52_12795"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNG60694.1"
                     /translation="MSDQSTERVFQNDIIQQMLANGWQLGSAEKYNRELALYPEDLIG
                     FVQQTQDAQWQKYKALYPHNAEQKLLELVGAQLNKADPNATHKDARTFGTLGVLRHEL
                     RDRNVRFNLCQFKPEHDLNPDTLARYHQNKLRIVQELVYSPWATDAELAETGTKAKAW
                     RIDLVLFVNGLPIATLELKSEFKQAVDNAIKQYRTTRFPICPTTKKPEPLLTFKRGAI
                     VHFAVSQYQVYMATQLNGATTYFLPFNKGTKEGAAGNDVPEDVNRYATDYLWNEVLLP
                     ENILNILARFVHLHIEEKEDWEGRKYKKESMIFPRYHQ"
     gene            complement(977..1960)
                     /gene="rhuM"
                     /locus_tag="PKZ52_12800"
     CDS             complement(977..1960)
                     /gene="rhuM"
                     /locus_tag="PKZ52_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNG60695.1"
                     /translation="MSENKVQLFTSADGVVSLEVSLDQETTWLSLDQMVYLFERDKSV
                     ISRHLRNLFAEGELERRSVVAKNATTAADGKTYQVDYYNLDVIISVGYRVKSQRGVQF
                     RQWATRVLKQHLVQGYTINQKRLAERGIEFEQAIALLSKTLSNQQLVAPAGEAVLGVI
                     SEYARSWSLLQGYDEQSLADIAQQQQGMIALDFTHALTAIAELKRELMAKGEATELFA
                     QLRGNGLESAIVTIEQGFGEEFFYPNIASRAAHLLYFVIKNHPLADGNKRTGSFLFLW
                     YLQINQHLLAKPVAQLINDNTLVALALLVAESLPDQKELMIRLVEHFILLK"
     gene            complement(1985..3292)
                     /locus_tag="PKZ52_12805"
     CDS             complement(1985..3292)
                     /locus_tag="PKZ52_12805"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNG60696.1"
                     /translation="MGRYQAYPEYKDSGVEWLGAVPQHWNIKRVGSIFEERREKVNDV
                     DFPPLSVTMQGIVPQLDNAAKTDAGDNRKLVMAGDFVINSRSDRKGASGISSLTGSVS
                     LISIVMSPRKYNSQFFHHLFRCRAFQEEFYRYGKGIVADLWSTNSSELKNILVPDLPQ
                     EEADRIANFLDHETAKIDTLIEKQQQLIQLLKEKRQAVISHAVTKGLNPNAPMKNSGV
                     EWTDQVPNHWKVTKLRYYAVISNGATPNRENTAFWENGNIGWLNSSKVNDQIIEFADQ
                     FITELAVLKTSVKAVRENDLVMAITGEGQTRGKVAVCKIDATINQHLASISITNNVLS
                     HEFLYIWFVANYERIRYESNGAGSTKGAITCGDIESFPVVIPPVGEQYEILELIKKET
                     LKFDQLEAKAISAIDLMQERRTALISAAVTGKIDVRHWQAPQN"
     gene            complement(3292..>4292)
                     /locus_tag="PKZ52_12810"
     CDS             complement(3292..>4292)
                     /locus_tag="PKZ52_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60697.1"
                     /translation="EADIATITRAFGDFEVINAYGLDKAADVKSNRGRQSATPKAEVP
                     KTFASKIFATHEFGYRRITIERPLRLSAQFTAEKIESLRFANGALKPVMEWIYGRISA
                     VWTDATYGQLSKHEEAIRSRIKKDFPDLKEKHIKEVLDAEIWLAQKALMKKAQQLRTE
                     IGDAQHDDFNEFDKTLTQAQKTLGIKLDAKELKQLLDAITWKNPEAEPVIKKVLKEKA
                     NPQYGKFTYKGKVVEFETDGDLRDNENVPLNPALSVNEINEAYFQNEVAPHVPDAWIN
                     ADKRDEKDGEIGIVGYEIPFNRHFYVYQPPRNLEEIDADLDKVTADILALLQEVHS"
BASE COUNT         1012 a          985 c          977 g         1318 t
ORIGIN      
        1 actggtggta acgcgggaag atcatgcttt cttttttgta tttacggcct tcccagtctt
       61 ctttttcctc aatgtgtaaa tgcacaaatc tggcgaggat atttaaaata ttctctggca
      121 ataagacttc attccagagg taatccgtag cgtagcgatt aacatcttcg ggcacatcgt
      181 tacccgctgc gccttctttt gtgcctttgt taaagggcaa aaagtaggtt gtggcaccgt
      241 ttagttgtgt ggccatgtaa acctgatatt ggcttacggc aaaatgcaca atggcgccgc
      301 gcttaaaggt taacagcggt tctggttttt tggtggtggg gcaaataggg aaacgggtgg
      361 tgcgatattg tttaatggca ttgtctaccg cttgtttgaa ttcagatttc aattccagcg
      421 tggcaatggg taaaccattt acaaacaata ccaaatcaat tcgccacgct tttgctttgg
      481 tgcccgtttc agccagttcc gcatccgttg cccaagggct atacaccagc tcttgcacta
      541 tgcgcagttt gttttggtgg tagcgcgcaa gggtgtctgg gtttaaatca tgctcgggtt
      601 taaattggca gaggttaaaa cgcacattgc ggtcgcgcag ttcatggcgc agtacaccga
      661 gcgtgccaaa ggtgcgggca tccttatggg tagcgttggg gtcggcttta ttcagttgcg
      721 cgcccaccag ctccaataat ttttgttcag cattgtgcgg gtaaagggct ttgtattttt
      781 gccattgcgc atcttgggtc tgctgcacaa aacctattaa gtcttcaggg tacaacgcga
      841 gttcgcggtt gtatttttca gccgaaccca actgccagcc gttagcgagc atttgttgaa
      901 taatgtcgtt ttgaaatacg cgctcggtgg attggtctga catgttgaat ccttctgtaa
      961 tctgtaatgt ttcgttttat ttcaacaaaa taaaatgctc aaccaagcgg atcattaact
     1021 ctttttgatc cggcaggctt tcggccacca gtaaggccaa agcgactagc gtgttgtcgt
     1081 taatcagttg tgcgactggt ttggctaata ggtgctgatt gatttgcaag taccacaaaa
     1141 acaaaaacga gccagtgcgt ttattaccgt cagccagcgg atggtttttg ataacgaagt
     1201 acagcaaatg cgccgcacgg ctggcgatat tggggtaaaa gaattcctcg ccaaaacctt
     1261 gttcaatggt gacgatggcc gactccaaac cattgccgcg cagttgcgca aataattccg
     1321 tggcttcgcc tttggccatt aactcgcgtt ttaattcagc gattgcggta agcgcatggg
     1381 taaaatccaa cgcaatcata ccttgctgct gctgggcaat atccgcaagg ctttgctcgt
     1441 cataaccttg gagtaaactc catgagcgcg cgtattcact gattacgcca agtaccgctt
     1501 cacctgctgg cgcgaccaat tgttggttag ataatgtttt ggaaagcagg gcaatggctt
     1561 gttcgaactc aatcccgcgc tcggcaaggc gtttttggtt gatagtgtag ccttgcacca
     1621 agtgctgctt aagcacacgg gttgcccact ggcggaattg cacaccgcgt tgcgatttaa
     1681 cgcggtagcc cacggagata atgacatcca gattgtagta atccacttgg taggttttgc
     1741 cgtcagcggc agttgttgca ttttttgcaa caactgaacg gcgctcaagc tcaccctctg
     1801 caaacagatt gcgtaggtgc cgtgaaataa ccgatttatc gcgctcaaat aggtagacca
     1861 tctgatcaag actcaaccac gtggtctctt gatcaagcga tacctccagc gataccacgc
     1921 catcggcaga ggtaaacagc tgaaccttgt tctctgacat ctggattccc ttagttctac
     1981 tattttagtt ttgtggtgct tgccagtggc gcacatcgat tttgccagtg acggcggcgg
     2041 agattaaagc tgtgcgacgt tcctgcatta agtcaatcgc agaaattgcc ttagcctcca
     2101 attggtcgaa cttaagcgtt tcctttttaa ttaactcaag aatttcgtat tgttcgccaa
     2161 ccggcggaat tacaacagga aaagactcta tatcaccaca tgtaatcgcg ccctttgttg
     2221 atccagcacc gttcgactca tatctaattc gttcatagtt tgcaacaaac cagatataca
     2281 aaaactcgtg cgatagaaca ttatttgtta ttgatataga tgccaaatgt tggttaattg
     2341 tggcatctat tttacagaca gcaacctttc ctcttgtttg cccctcgccc gtaattgcca
     2401 taacaagatc gttttctctt acagctttta cactggtttt taaaactgcc aactcagtta
     2461 taaactgatc agcaaactct attatctggt catttacttt tgaactgttt aaccatccaa
     2521 tatttccatt ttcccaaaaa gctgtgtttt cgcgatttgg tgttgcacca ttcgatatca
     2581 ctgcgtagta acgtaactta gtcactttcc aatggtttgg aacttggtct gtccattcca
     2641 ccccagaatt tttcataggt gcattaggat taaggccttt ggtgacggcg tggctgataa
     2701 cggcttggcg tttttctttt aggagttgaa tgagttgttg ttgcttttcg atcagcgtgt
     2761 cgatttttgc ggtttcgtga tcgaggaagt tggcgatcct atctgcctct tcctgcggaa
     2821 gatcaggaac caatatattc ttgagttcag atgaattggt actccataaa tcagcaacta
     2881 tccccttccc gtatctatag aactcttctt gaaacgctcg gcatctaaaa agatggtgaa
     2941 aaaattgcga gttatatttt cgcggcgaca tcacaatact gattaaagac accgaaccag
     3001 ttagagatga aattccggat gctcctttcc tatcagaacg actatttatt acaaagtctc
     3061 cagccataac caattttcga ttatcaccag catcggtttt ggcagcatta tccaattgag
     3121 gaactatacc ttgcatagtc acagacaggg gcggaaaatc aacatcattt actttttcac
     3181 gcctttcttc aaaaatagat ccgacacgct ttatgttcca atgctgaggg actgccccta
     3241 accactccac accagaatcc ttatattctg gataagcctg ataacgcccc attacgaatg
     3301 cacctcttgt aacagcgcca aaatatccgc cgttacctta tccaaatccg catctatctc
     3361 ttccaaattg cgcggcggtt ggtacacata aaaatggcgg ttaaacggaa tttcgtaacc
     3421 gactattcct atttcgccat ctttttcatc gcgcttatct gcattaatcc aagcatctgg
     3481 cacatggggt gccacttcat tttggaagta ggcttcgttg atttcattta cggatagcgc
     3541 agggttgagc ggtacgtttt cgttatcgcg caggtcgccg tcggtttcaa attctacgac
     3601 tttgcctttg taggtgaatt tgccgtattg cgggttggct ttctctttca gcactttttt
     3661 aatgacgggt tcggcttctg gatttttcca agtgattgca tccagcaatt gttttagctc
     3721 tttggcatcc aactttatgc ccagcgtttt ttgcgcttga gttagggttt tgtcgaactc
     3781 gttaaaatcg tcgtgctgtg catcgccaat ttcggtgcgc agttgttggg cttttttcat
     3841 aagggctttt tgtgcgagcc agatttcggc atccagtacc tctttgatgt gtttttcttt
     3901 taagtctggg aagtcttttt tgattctact acggatggct tcttcatgtt tgctcagctg
     3961 cccgtaggtg gcgtcggtcc atacggcgct gatgcggcca taaatccact ccatcactgg
     4021 ctttaatgcg ccgttggcga aacgcaggct ttcaattttt tcagccgtga attgtgcgga
     4081 taagcgcaat ggccgctcga tggtaatgcg gcgataaccg aactcgtggg ttgcgaatat
     4141 tttgctggcg aaggttttgg gaacttccgc ttttggggtt gcagattggc gaccacgatt
     4201 gctttttaca tcggctgctt tatctaaacc ataggcattg atcacttcaa aatcaccaaa
     4261 ggcgcgggtg atggtggcta tgtctgcttc at
//
LOCUS       DAOQGS010000357         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11155_length_4268_cov_4.458106, whole genome shotgun sequence.
ACCESSION   DAOQGS010000357 DAOQGS010000000
VERSION     DAOQGS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11155_length_4268_cov_4.458106"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKZ52_12815"
     CDS             <1..1286
                     /locus_tag="PKZ52_12815"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60698.1"
                     /translation="VGKSEDGGVNVVLLIQVPLRQRPRPVSYGAIGAAGMDAMEMERG
                     ISLPPEHWQRVLDVYASKGLEAGLLKRALIIDFAARMQLPIPTLLKGLLEDLDKQSAS
                     KPSQEAPLDDSASLVMAAPDSALGRAAARVIADGLAKAVNLPTSAAHQAALPEALATL
                     AASELNIAKAIALYRKPYEPDAAQALLARLAPEHATLERALILSWLDQAAAASPKAPL
                     VTLSGDWRAHKRKAASDTWRWAGKGLPASVELTTAPAETVPAVVNFISAEAPPAVDAS
                     LSISRSLHKLTPGDKALNFATQPVADGSLATNALYLDEILVKYSGKKPLRYALLEVPL
                     PPGADVERTTWGIEVTFAGATEAVVLEKAQAEDGALSYAVPLEQVSGEVRIRHLVRFG
                     QKGSFQLPPARLKDMYNPARAYYEQAPKYSRVQVN"
     gene            1290..3056
                     /locus_tag="PKZ52_12820"
     CDS             1290..3056
                     /locus_tag="PKZ52_12820"
                     /inference="COORDINATES: protein motif:HMM:NF021572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HNG60699.1"
                     /translation="MPVELKRRLRYWFAGACCALLAALPLGKALAQPEGFAQPGLLHL
                     VLAQQGKLTYWQLASSGRASRQPLPQNQQVPLGSLWKLWVYAYLADSGAGASAANAET
                     PYTCQGNNPEEAYCCSAKGEQVGRDLALAKSCGLYFSPSRLNLDAAHWRTYWQSRGLP
                     QALLDLGHLTPDQQVPLAELLNSLAQLPAQEQAKRALLQVLLSPQGKPHAARVGSRLR
                     IKTWSWRDEAANPIGGFAGWLADGTVLWAQAEGTSAQVLSRYGAALNTHLPNPPPIHD
                     TSCVQVQLFAAYPIKTVLDAQGQTAKAGPLQGQFRVEFVKGTALTFTSHGETRLGTTD
                     TGQPQLTADLTQAEYIARVLEREAAAQPVEAAKALAIAARTYLIQNARRAPDPRGTGD
                     GQGECWQIDDSSQTQRVSPSPATAKTRAIADWTADLISSGASVRYHLDQPGSNRLVWK
                     TAVQQAQQGFSFYEILNLAFPQNDLTSEYSSTSLCQPLPQARAWLAGQIPKWRAALLS
                     EPGFAPPPLPQLCQLPSGNPFNDTARNRIFARGHYSLQQRLDITHEYLHLAFADYPSG
                     QDETYIEQLARRLLLGQELSHD"
     gene            3049..3858
                     /locus_tag="PKZ52_12825"
     CDS             3049..3858
                     /locus_tag="PKZ52_12825"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HNG60700.1"
                     /translation="MTNHYLKAIMVTSLSLLALGAASSGPAEPAQDAAPELKTPINGW
                     RPKVEKGGQFTQQVNYPAASVNTPAGQADTARIRGAIAATPKNTAGPATLVVNGVAMP
                     LSSNPDGSFDRPYIFTSGSNSLEARAPSGASKRVQFYQQAGNETRPQLRIVLSWDSDN
                     TDLDLHVITPDGEHAWYGNRVLSNGGAQDMDVTTGFGPEIFASPSPLPGQYLVYVNYY
                     GGSTDEDAQPITSARVTLITHEGTADEKIETTLVPMRAAGELNFVKRFSYP"
BASE COUNT          926 a         1407 c         1212 g          723 t
ORIGIN      
        1 cggtgggcaa gtccgaggac ggcggggtca acgtggtgct cctgatccag gtgccgctgc
       61 gccagcgtcc gcgccccgtg tcctacggcg ccatcggcgc ggcggggatg gacgccatgg
      121 agatggagcg cggcatcagc ctgccgccgg agcactggca gcgggtgcta gatgtatatg
      181 ccagcaaggg cttggaggcg ggcctgctga aacgagcgct gattattgat tttgccgccc
      241 gtatgcagct gcccatcccc accctgctga agggattgct ggaagatttg gataaacaaa
      301 gtgccagcaa acctagccaa gaggcgcccc tggacgacag cgcaagcctg gtcatggccg
      361 cgccggattc cgcccttggc cgcgccgcag cccgagtaat cgccgatggc ctggccaaag
      421 cggtgaacct gcccaccagc gccgcccacc aggcggcatt gcctgaggcc ctagccaccc
      481 tggcggcaag cgagctgaat atagccaagg ccattgccct ctaccgcaaa ccctatgagc
      541 cagatgccgc ccaagccctg ctggcgcggc tcgcccctga gcacgcgact ttggagcggg
      601 cgctgatcct cagctggctg gatcaagccg ccgcagcctc ccccaaagca cccttggtga
      661 ccctcagcgg tgactggcgc gcccacaaac gcaaagccgc cagcgacacc tggcgctggg
      721 cgggcaaagg cttacctgca agcgtagagc tgactaccgc ccccgccgag accgtgcctg
      781 cagtggtgaa ctttatcagc gccgaggcac cgcctgcagt ggatgccagc ctgagcatca
      841 gccgcagcct gcacaaactc acccctggcg ataaagccct caacttcgcc acccagccgg
      901 tggccgatgg cagcttggcc accaacgccc tctacctgga tgaaatccta gtgaaataca
      961 gcggcaaaaa acccctgcgc tacgccctat tagaagtacc cctgcccccc ggcgccgatg
     1021 tggagcgcac cacctggggc attgaagtga cattcgcagg cgccaccgaa gcagtggtgt
     1081 tggaaaaggc ccaggcggag gacggcgccc tcagttacgc cgtgccctta gagcaggtga
     1141 gtggcgaggt gcgcattcgt catctggtgc gctttggcca gaaaggcagc ttccaactgc
     1201 caccggcgcg gttaaaagat atgtacaacc cggcgcgggc ctattacgag caggcgccta
     1261 agtacagccg cgtgcaggtg aattaagcct tgccggtaga gcttaaaagg cgccttaggt
     1321 attggtttgc cggggcctgc tgcgcgctgc ttgccgccct gccactgggc aaagccctcg
     1381 ctcagcccga gggctttgcc cagcctgggc tattgcatct ggtgctcgcc cagcaaggca
     1441 agctcaccta ttggcagctg gccagctccg gccgggcaag ccgacaaccc ctgccccaaa
     1501 accaacaggt gcccttgggc agcctgtgga aactctgggt gtacgcctat ctggcggaca
     1561 gcggcgcagg cgccagcgca gccaacgcag aaacccccta cacctgccag ggtaacaacc
     1621 cggaggaggc ctattgctgc agcgccaagg gcgaacaagt ggggcgggat ctggccctgg
     1681 ccaaaagctg cggcctctac ttcagcccca gccgcttaaa cctggatgcc gcacactggc
     1741 gcacctattg gcagagccga ggcctgcccc aggcgctact ggatctgggc cacctaaccc
     1801 ccgaccagca ggtaccgcta gcggagctgc ttaattccct agcccaactg cccgctcaag
     1861 agcaggccaa gcgcgccttg ctgcaggtgt tattaagccc ccagggcaag ccccatgcag
     1921 ccagagtcgg ctcccgctta agaattaaaa cctggagctg gcgggatgaa gcggccaatc
     1981 ccatcggcgg ttttgccggc tggctggccg atggcactgt cctctgggcc caggccgagg
     2041 gcactagcgc ccaggtgtta agccgttatg gggcggccct taacacgcac ctgcccaacc
     2101 caccgcccat ccacgacaca agttgtgtac aggtacaact gttcgccgcc taccccatca
     2161 aaactgtgtt ggatgcccag ggccaaaccg ctaaagctgg gccgttgcag gggcaattcc
     2221 gggtggaatt tgtgaagggt actgccctca cttttaccag tcatggggag acgcgcctgg
     2281 gcacaaccga taccggccaa ccgcaactca cggctgatct aacccaagcg gagtacatag
     2341 cccgcgtcct tgagcgcgaa gcggcagcgc agcctgtgga ggcagccaag gccctggcca
     2401 tagccgcccg cacctacctg atacaaaacg cccgccgcgc gccggatccc aggggcacag
     2461 gtgatggcca gggggagtgt tggcaaatcg acgacagcag ccaaacccag cgggtcagcc
     2521 cgagcccagc cacagccaaa acccgcgcca tagccgattg gacggcggat ctgatcagca
     2581 gcggcgccag tgtgcgctac cacctggacc agccaggctc caaccgcctg gtctggaaaa
     2641 ccgccgtgca gcaggcccag caggggttca gcttctacga gattttaaat ctcgccttcc
     2701 cgcaaaacga tctgacgagt gaatacagca gcaccagcct ctgccagccc ctgccgcaag
     2761 cgcgcgcttg gctggccggg caaatcccca aatggcgggc cgccttgcta agcgagccgg
     2821 gttttgcgcc gccgccattg ccgcaactgt gccagttgcc cagtggcaat ccattcaatg
     2881 atacggcccg caaccgcatc tttgcccgcg gccactacag cttgcagcag cgcctggaca
     2941 tcacccacga atacctacac ctggcctttg ccgattaccc cagcggccag gatgaaacct
     3001 acatcgaaca actcgcccgc cgcctgttgc tgggccagga gctaagccat gactaatcac
     3061 tatctcaaag caattatggt caccagccta agcctgcttg cgctcggcgc cgccagcagc
     3121 ggcccggccg aacccgccca ggatgccgcc cccgagctta aaacccccat caatggctgg
     3181 cggcccaaag tggagaaggg cggccaattc actcaacagg tcaactaccc cgccgcctcg
     3241 gtcaatacgc cagcaggcca ggccgacacc gcccgcatcc gcggtgccat agctgccacg
     3301 ccgaaaaaca ccgcagggcc cgccacccta gtggtaaatg gcgttgccat gccgctgtcc
     3361 agcaaccccg acggcagctt tgatcgccct tacattttca cctccggcag caacagcctg
     3421 gaggcgcgcg cccccagcgg cgcctctaaa cgggtgcagt tttaccaaca ggcgggcaat
     3481 gaaacccgcc cacagctgcg cattgtgttg tcctgggatt cggacaatac agacctagac
     3541 ctgcacgtca tcaccccgga tggggagcac gcctggtacg gcaaccgggt attaagtaac
     3601 ggcggcgccc aggatatgga cgtcaccacc ggcttcggcc cggaaatctt cgcaagcccc
     3661 agccccctgc ccggccagta tttggtatac gtgaactatt acggcggcag caccgacgag
     3721 gacgcccaac cgataaccag cgcccgcgtg acactgatta cccacgaagg cactgccgat
     3781 gaaaaaatcg aaaccaccct ggtgcccatg cgggcggcgg gggaattgaa ttttgtgaag
     3841 cggtttagtt atccctagtt gttaagcacc gttttatgca gcaagtcgtg tttgcgaaac
     3901 caataggcga tggtttagtg ctgcaagcgc tcgatacccc ttccgcaaag aaaaatacca
     3961 ctgggtaaat ttctcgttaa atttgccgcc tgatagtaag cattaaaaga tgtgaagatt
     4021 agttgcgtga ataatctgct gctttattaa gtgcggacag agtgcaaccg gaactgttca
     4081 gattactttt ccgacaatat caattaaaaa tattttgtaa ctgtccaaca caccttatta
     4141 ccctctcagc acatctgttt accctcttcc gcactggcgg caaattctgg gggattagct
     4201 atacggcaga attgattcgc ggtgtagcag tggtgactac aggttagtgc agaccgtcaa
     4261 aaagaggg
//
LOCUS       DAOQGS010000358         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11166_length_4264_cov_5.227608, whole genome shotgun sequence.
ACCESSION   DAOQGS010000358 DAOQGS010000000
VERSION     DAOQGS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11166_length_4264_cov_5.227608"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKZ52_12830"
     CDS             <1..784
                     /locus_tag="PKZ52_12830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNG60701.1"
                     /translation="SSSSSSSSSSSSSSSSGRSSSSSSSSSSSSSSSSSSSSSSSGAL
                     SKYCPTNATCKILPLGDSITDGLTVAGGYRIELFRLSMKDGMDITFVGGSKNGPATVD
                     GVAFPQSHEGHSGWTIAQIDDIVQNKNALGVNPNIILLHIGTNDMYGSDPANADKRLA
                     TLLDHIIAKQPNALLAVSNIIPLGILATAEVPKYNAKIPAVLNARIQAGKNIIFVDQY
                     KDFPTSELQDQVHPNSAGYARMATKWYAAIKPYLVDINNITP"
     gene            1037..>4264
                     /gene="hsdR"
                     /locus_tag="PKZ52_12835"
     CDS             1037..>4264
                     /gene="hsdR"
                     /locus_tag="PKZ52_12835"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HNG60702.1"
                     /translation="MSTANTNSNFSFLAEHDPLLVELAQAAERAFASDPNTTLIKLRQ
                     LGEALAQHIAALSGVEFDEQTTQADLLYKIGRELKLDATVRDLFHILRVEGNKATHQF
                     KTQHKEAISGLIVARKLAIWFHQAFGKVGTQFKPGPFVPPTDPSEQLRKLQTQIAQLK
                     NDLQQANQDLGSNQTLTELIRREKDEYEALAIAMDEEARTLAQQAASHEKALAQQRQD
                     YETKIKNLQAQIAALDEKHQATQLHTLNQNTQAATAQIVLDEALTRILIDADLTAAGW
                     QADSEQLTYKNGTRPEERKNIAIAEWPTLYKSEKGRADYVLFAGLTPIAVVEAKRENI
                     NVAGKITQAERYSQGLSVLPPLIGAWELAGQTVAWPDASDGHYKVPFVYSCNGRPYVP
                     QLAEQSGTWFRDVRHASNTKRALQSFHTPAGLIDLLKRSKEDAEKKLKAEPFGYLKLR
                     EYQQKAILAVEDTLERDARSALLAMATGTGKTRTIIGLMYRFLKAERFKRILFLVDRT
                     ALGQQALDSFYEVPLEQAFTLKKNYVIAEMADMAVEAATRIQVATVQAMVKRIFTSDN
                     PPPVDQFDCIIVDEAHRGYTLDQEMTEGELATRDASQYLSSYRRVLDYFDAFKVGLTA
                     TPAKHTSDIFGHPVFIYSYREAVADDWLIDHEPPIRYETLLTQNGIHFAKGETVSSID
                     TQTGEVLSSELDDELNFDVDAFNRRVINENFNKVICEQLVQELDPFGEEKTLIFCATD
                     LHADMVKRLLDDAFKDMYNGKYNQAAVAKITGQSDKVGQLIKQFKNERYPNIAITVDL
                     LTTGIDVPKICNLVFMRRVKSRILYEQMIGRATRRCDEIGKTVFRIYDPVDIYATLAA
                     VNTMKPLVKDPNITLEQLVAELTDPQQLEKALNAPGEQPGESHAHMVLSQLSQKVMRV
                     LRKAEKKAEFKPELKNKLAELHGLWGVEPKTLHQHLHSLGPQQAAAFISQHNGLLNQI
                     SQVATLLGSEYQPVISQHEDEIRERSQSFGPHEKPEDYLQNFAHFIKNQLNQSAALSV
                     VVNKPRDLTRQQLKEIKLLLDGAGFSEANLRSAVRNQTNQDIAASI"
BASE COUNT         1241 a         1159 c          948 g          916 t
ORIGIN      
        1 aagctcatca agcagttcaa gttctagcag ttcatccagc tcaagttccg ggcgcagttc
       61 atccagcagt tccagctcta gtagttcatc ttcgagcagc tcctccagca gttcttcatc
      121 atccggtgca ctttctaaat actgcccaac caatgcaacc tgcaaaattc tgccactggg
      181 tgattccatt accgatgggc ttaccgtggc cggtggttac cgcattgaat tgttccgctt
      241 gagcatgaaa gacggcatgg atattacgtt tgtgggcggc tctaaaaatg gcccggcaac
      301 tgtagatggc gtagcctttc cgcaatccca cgagggccac agcggttgga ccattgcgca
      361 aatcgacgat attgtgcaaa acaaaaatgc cctcggcgta aaccccaata ttattttgct
      421 gcatattggc accaacgata tgtacggctc cgaccctgcc aatgccgata aacgcttagc
      481 caccctgctc gaccacatca tcgccaaaca acccaatgct ttattagcgg tttcaaacat
      541 tattccactg gggattttag ccacggcaga agtgcccaaa tacaacgcca aaatccccgc
      601 tgtattaaat gcgcgaattc aagcgggcaa aaatattatc tttgtagacc aatataaaga
      661 tttccccacc agtgaattgc aagatcaagt tcaccccaat agcgcaggct atgcccgcat
      721 ggctacaaaa tggtacgcgg ctattaagcc atatttagtg gatattaata acattacgcc
      781 ctaattaacc atcgcagaca acccactctt acgcttaaac ccaaccaatg ggtaagcgta
      841 agggtccagt tttattccca cacattcagc acccagcgct acgcaattat aaacgtaatc
      901 ccccttcccc ccggcttaca ctgatcgtga taagctctcc tctccttgca ggcggcaccg
      961 tgcccgcagc actagcacct aaataaaatt aataggccct gcgcatccat catgaacaac
     1021 atggagtttc gtatccgtga gcactgccaa cacaaacagt aatttcagct ttctggccga
     1081 gcacgatcca ttattggtgg aactggccca ggcagccgag cgcgcatttg cctcagaccc
     1141 caataccact ctaataaaat tgcgccagct tggcgaagcc ctggcccaac atatcgccgc
     1201 gctgagcggg gttgaatttg atgaacaaac cacacaagcg gacctgctgt ataaaattgg
     1261 cagggaactc aagcttgatg caaccgtgcg cgatttattc catatcctgc gcgttgaggg
     1321 taataaggcc acccaccaat ttaaaaccca acacaaagag gccatcagcg gccttatagt
     1381 tgcgcgcaag ctagctattt ggttccatca agcatttggt aaagtcggca cacaatttaa
     1441 acccggcccg tttgtacccc ctactgaccc cagcgaacaa ctacgtaaat tacaaacaca
     1501 aatcgctcag cttaaaaatg atctgcaaca agccaatcaa gatttaggtt ccaaccagac
     1561 ccttaccgaa ttaattcgcc gggaaaaaga cgaatacgaa gcgctggcca ttgccatgga
     1621 tgaagaggca cgtactctgg cgcagcaagc agccagccac gaaaaagccc tggcacaaca
     1681 gcggcaagac tatgaaacta aaatcaaaaa cctgcaggca caaatagctg cgctggatga
     1741 aaaacaccag gccacacagt tacacacgct taaccaaaat acccaggcgg ccacagcaca
     1801 aattgtgctg gacgaagctc taacccgcat tcttatcgac gccgatttaa ccgccgcagg
     1861 ctggcaggcc gatagtgaac agctcaccta caaaaatggt acccgcccgg aagagcgcaa
     1921 aaatatcgcc attgccgaat ggcccaccct ttataaaagt gaaaaaggcc gggcggatta
     1981 tgttttgttt gcaggcttaa cgcctatcgc agtggttgag gctaaaagag aaaatattaa
     2041 tgtggccggc aaaattactc aggccgaacg ctacagccag ggcttaagtg tattaccgcc
     2101 gttaattggc gcttgggaat tggccgggca aaccgttgcc tggccggacg ccagtgacgg
     2161 ccactataaa gtgccgtttg tttactcgtg taatggccgc ccctatgtgc cgcaattggc
     2221 ggagcaatct ggcacctggt ttcgcgatgt acgccatgcg tctaatacca agcgtgcgct
     2281 acaaagtttt cacaccccgg caggattaat tgatttactc aaacgcagca aagaagacgc
     2341 cgagaaaaag cttaaagccg agccattcgg atatttaaag ctgcgcgagt accaacaaaa
     2401 agccatttta gccgtggagg ataccctcga gcgcgatgcg cgcagtgcat tattggccat
     2461 ggccaccggc acaggtaaaa cccgcaccat catcggttta atgtatcgat ttttaaaagc
     2521 cgagcgcttt aaacgtattt tatttttagt ggaccgcacc gctctgggcc aacaagcgct
     2581 ggattcgttt tatgaagtac cactggaaca agccttcacc ttaaaaaaga attatgtcat
     2641 cgcggaaatg gccgacatgg cggtagaagc ggcaacccgt atacaagtgg ccaccgtaca
     2701 agccatggtg aagcgcattt ttaccagcga taacccgccg ccggtggatc aatttgactg
     2761 catcattgtg gatgaagcac accgaggtta cactctggac caagaaatga ccgaagggga
     2821 actagccacc cgggatgcca gccaatacct atccagttac cgccgagtgt tggattactt
     2881 cgacgctttt aaagtgggct taactgccac accggcaaaa cataccagcg atatttttgg
     2941 ccacccagta tttatttatt cgtaccgcga ggcggtggca gatgattggt taattgacca
     3001 cgagccaccc atccgctacg aaaccctact tacccaaaac ggcattcact ttgccaaagg
     3061 cgaaaccgtc agctccattg atacccaaac cggtgaggta ttaagtagcg aattagatga
     3121 cgaattaaat ttcgatgtgg acgcttttaa ccgccgggtc attaacgaga attttaataa
     3181 agttatctgc gagcaattgg tccaagaatt agatcccttt ggcgaggaaa aaactctaat
     3241 cttctgcgcc acggacctgc acgcggacat ggttaaacgc ctgctggatg atgcctttaa
     3301 agatatgtac aacggcaaat ataaccaagc ggccgtagcc aaaataaccg gccaaagcga
     3361 taaagtgggc caactgataa aacaatttaa aaacgagcgc tacccgaata tcgccattac
     3421 cgtggatttg ctcaccaccg gtatagacgt tcccaaaatt tgtaatctgg tctttatgcg
     3481 ccgggttaaa tcgcgcattt tgtacgagca gatgatcggc cgcgccaccc gccgctgcga
     3541 tgaaataggt aaaaccgttt tccgcattta cgacccagtg gacatctacg ccaccctggc
     3601 cgccgtaaac accatgaagc ccctggtgaa agacccgaac attacccttg aacaattggt
     3661 ggcagaactc accgacccgc aacagttaga aaaagccctg aatgcaccgg gtgagcaacc
     3721 gggcgaaagc catgcccata tggtgcttag ccaattaagc caaaaggtga tgcgggtttt
     3781 gcgcaaagcc gagaaaaaag ccgaatttaa accggagcta aaaaataaac tggcggaatt
     3841 acacggccta tggggtgtag agcctaaaac cttgcaccag catttacaca gcctaggccc
     3901 ccagcaagcg gcggcattta ttagccagca taacgggctg ttaaaccaaa taagccaggt
     3961 agcaaccctg ctgggcagtg aatatcaacc ggtaatttcc cagcatgagg atgaaattcg
     4021 cgagcgcagt cagagtttcg gcccccacga aaaacccgag gattatttac aaaactttgc
     4081 tcactttata aaaaaccaac tcaaccaaag tgctgctctt tccgtggtgg tcaacaaacc
     4141 ccgcgacctc acccgccagc agctcaaaga aattaaatta ctgctcgatg gcgcaggttt
     4201 cagtgaagcc aatttacgca gcgccgtgcg caaccaaacc aaccaggaca tagccgccag
     4261 catc
//
LOCUS       DAOQGS010000359         4261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11176_length_4261_cov_3.924631, whole genome shotgun sequence.
ACCESSION   DAOQGS010000359 DAOQGS010000000
VERSION     DAOQGS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11176_length_4261_cov_3.924631"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKZ52_12840"
     CDS             <1..436
                     /locus_tag="PKZ52_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNG60703.1"
                     /translation="RIPVIANGDITRAAQAQAVLAATGAAGVMVGRAAQGNPWIFREI
                     NDYLQGTQSPRAPSQEEILTTVLHHLEGIYALYGQEAGVRIARKHFAWYLAALSPLGA
                     VFSPEGSAELRRQFNQVQSTQEQQQLARTLFGRIKPLEDQAA"
     gene            433..747
                     /gene="fis"
                     /locus_tag="PKZ52_12845"
     CDS             433..747
                     /gene="fis"
                     /locus_tag="PKZ52_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HNG60704.1"
                     /translation="MNTVDPILTDRTREISIKPIAPTPANTNSGQSLRDYVETAVSNY
                     FQHLEGQDVTNVYEMVLSEVETPLLEVVMKYTRHNQTRAAEILGLNRGTLRKKLKQYG
                     LL"
     gene            866..2455
                     /gene="purH"
                     /locus_tag="PKZ52_12850"
     CDS             866..2455
                     /gene="purH"
                     /locus_tag="PKZ52_12850"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNG60705.1"
                     /translation="MSSPNLVPVKRALISVSDKTGIVAFAQRLHSLGVELLSTGGTYQ
                     LLKQQGIPVVEVSSYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAVMTEHGIKPIDM
                     VVVNLYPFEQTVAKPDCSLEDAIENIDIGGPTMVRAAAKNHAHVAIVVNSSSYDQVLD
                     ELAANQGKLGLATRFDLAVQAYEHTAAYDGAIANYLGTKLAQPEGSEGSRYPRTFNSQ
                     FIKAQDMRYGENPHQTAAFYVEKSPQDACIATAKQLQGKELSYNNVADTDAALETVKL
                     FSRPACVIVKHANPCGVAEADDLLSAYNKAYATDPESAFGGIIAFNRELDGATASAIV
                     ERQFVEVIIAPSVTPEASAIVSSKKNVRLLECNYWKQPGLAGFDFKRVSGGLLVQERD
                     LGLVSEANLEVVTTRKPTPTEIADLLFAWKVAKMVKSNAIVYAKDQTTIGVGAGQMSR
                     VNSARIAAIKAEHAGLQVAGSVMASDAFFPFRDGLDNAASVGIAAVIQPGGSVRDDEV
                     IAAANEHGIAMVFTRMRHFRH"
     gene            2478..3782
                     /gene="purD"
                     /locus_tag="PKZ52_12855"
     CDS             2478..3782
                     /gene="purD"
                     /locus_tag="PKZ52_12855"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNG60706.1"
                     /translation="MKILIIGSGGREHALAWKVAQNPAVSQVFVAPGNAGTATEAKVR
                     NLALDALNFPALAEFVQSEGVALTIVGPEAPLVAGIVDYFAERNLACFGPRKGAAQLE
                     GSKAFTKDFLARHQIPTAAYQNFSEVEPALAYLRSQGAPIVVKADGLAAGKGVIVAMT
                     LSEAEDAVRDMLSGNAFGDAGCRVVIEEFLDGEEASFIVMADGVHALPMATSQDHKRI
                     GDGDTGPNTGGMGAYSPAPVVTPEVHARVMEQIILPTLRGMAFEGNAYTGFLYAGLMI
                     DGAGNPKVIEYNCRFGDPETQPIMLRLQSDLVALCQAALAGNLAQTDAQWDPRPAVGV
                     VLAAGGYPNDYAKGDVIEGLEAAAALPGKVFHAGTQQKDTAQGQAIVTAGGRVLCATA
                     LGESVTLAQQAAYRLADAISWPGCTKRRDIAYRAIARESARG"
     gene            3943..>4261
                     /locus_tag="PKZ52_12860"
     CDS             3943..>4261
                     /locus_tag="PKZ52_12860"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG60707.1"
                     /translation="MTDDTAAETQVLVLAAESSRKRFRLENDMLVGREPSCDIPINSL
                     HISRQHARITQTPTGFFLEDLNSANGTYINGLKLTTPVALQIGDLVCFDDQGFRVVAE
                     GKVE"
BASE COUNT          949 a         1364 c         1146 g          802 t
ORIGIN      
        1 gcgcatcccg gtcatcgcca atggcgatat caccagagcc gcccaagccc aagcagtgct
       61 ggcagcaact ggcgcggcgg gcgtcatggt ggggcgagcc gcccagggca atccctggat
      121 ttttcgtgaa attaacgact atttacaggg cacccaaagc cccagagccc catcccagga
      181 ggagatactc accaccgttt tacaccacct tgagggtatc tatgccctct atggccagga
      241 ggctggcgtg cgcatcgcac gcaaacactt tgcttggtat ttagcagccc tatccccgtt
      301 aggtgcggtc ttttccccgg aaggcagcgc tgaactacgc aggcaattta atcaggtcca
      361 atcgacccaa gaacaacaac aattagcccg aaccctgttt gggcgcatta aaccattaga
      421 ggatcaagcc gcatgaatac cgtcgacccc atcttgactg accgcacgcg tgaaatcagc
      481 atcaagccca tcgccccaac ccccgccaat accaacagcg gccagtccct gcgcgattac
      541 gttgaaaccg ccgtcagcaa ctactttcag cacttagaag gccaagacgt caccaatgtt
      601 tatgaaatgg tgctctctga agtggaaacg cccctgttag aagtggtcat gaagtacacc
      661 cgccacaacc aaacccgcgc cgccgaaatc ctcggcctca accgtggcac cctgcgcaaa
      721 aaactcaagc aatacggcct gctttaatcg cgctatccat ttaagtttta tcgcgccgca
      781 ccactgcggc ttatgcccgg cccccagata ggtgaggccg ggttttattg tttaatctac
      841 tttctcctga ggactttgct cgctcatgtc gtcccccaat ctagttcccg tcaaacgcgc
      901 gctgattagc gtatcggata aaaccggcat agtggctttt gcccaacgcc tgcacagcct
      961 gggtgttgaa ctgctctcca ccggtggcac ctaccagcta ctcaagcaac agggcatacc
     1021 tgtggttgaa gtgtcttcgt acaccggctt tcccgagatg atggacggcc gcgtaaagac
     1081 ccttcacccc aaagtacacg gcggcatatt ggggcggcgc gggcaggacg acgcggtaat
     1141 gaccgaacac ggcatcaagc ccatcgatat ggtggtggtg aacctctacc cgtttgagca
     1201 aacggtggcc aaacccgatt gctctttgga agacgccatc gaaaacatcg acataggcgg
     1261 gcccaccatg gtgcgcgccg ccgctaaaaa ccatgcccat gtggctatag tggttaacag
     1321 cagcagctac gaccaggtac ttgatgagct tgccgccaac cagggcaagc tgggtcttgc
     1381 cacccgcttt gacctggccg tacaggccta tgaacacact gcagcctacg atggcgccat
     1441 cgccaattac ctgggcacta agcttgccca gcccgagggc agtgaaggca gccgctaccc
     1501 gcgcaccttt aacagccaat ttatcaaagc ccaagacatg cgctacggcg agaaccccca
     1561 ccaaaccgcc gcgttttatg tggaaaaatc cccccaagac gcctgcatcg ccaccgccaa
     1621 acagttacag ggcaaagagc tgtcctacaa caatgtggcc gataccgacg ccgccctgga
     1681 aaccgtcaag ctgtttagcc gcccagcttg cgtcatcgtc aaacacgcca acccctgcgg
     1741 tgtggccgag gccgacgacc tgttgagcgc ctacaacaag gcctatgcca cggaccccga
     1801 atccgccttt ggcggcatca tcgcctttaa ccgcgagttg gatggcgcca ccgccagcgc
     1861 catagtcgag cgccagtttg ttgaggtgat catcgccccc agtgtcaccc cggaggccag
     1921 cgccatcgtc agcagcaaaa agaatgtgcg gttactggag tgcaactact ggaaacagcc
     1981 aggcctggcc ggttttgact tcaaacgagt tagcggcggt ctcttggtgc aagagcggga
     2041 tttaggcttg gtgagcgagg ccaatctgga agtggtcacc acccgcaaac ccaccccgac
     2101 tgaaattgcc gacctgttat tcgcctggaa agtggccaag atggtgaagt ccaacgccat
     2161 cgtttatgcc aaggatcaaa ccaccatagg tgtgggcgca ggccaaatga gccgggtcaa
     2221 ctccgcccgt atcgccgcca tcaaagccga gcatgcaggc ttgcaggtgg ccggttccgt
     2281 tatggcttcc gacgccttct tccctttccg ggacggcctg gacaacgccg caagcgtagg
     2341 catagcggcc gtgattcaac ccggcggctc cgtgcgggat gacgaggtga tcgccgccgc
     2401 caatgaacac ggcattgcca tggttttcac ccgtatgcgc cacttccgcc attgatcagc
     2461 cgctctagga gcaacctatg aaaattctca ttatcggcag cggcggccgc gaacacgccc
     2521 tggcctggaa ggtcgcccaa aaccctgccg tcagccaggt gtttgtcgcc cccggcaatg
     2581 cgggcaccgc caccgaagct aaggtgcgca acctggccct ggacgcttta aacttcccgg
     2641 ccctcgccga atttgtgcag agtgagggcg tcgccctcac catcgttggg ccagaagcgc
     2701 ctttggtggc aggcattgtg gattattttg ccgagcgcaa cctcgcctgc tttggccccc
     2761 gcaagggcgc cgcacagctg gaaggctcca aagccttcac caaagatttc ttagcccgcc
     2821 accagatccc caccgccgcc tatcaaaact tcagcgaagt agaacctgct ctggcctacc
     2881 tgcgcagcca aggcgccccc atagtcgtga aggccgatgg cctggcggcg ggcaagggcg
     2941 tgattgtggc catgaccctc agcgaggccg aagacgccgt gcgcgatatg ttatccggca
     3001 atgcttttgg cgatgctggc tgccgagtgg taattgaaga attcctcgat ggcgaggagg
     3061 cgagttttat cgtcatggcc gatggtgtac acgccctgcc catggccacc agccaagacc
     3121 acaagcgcat cggcgacggc gataccggcc ccaacaccgg cggtatgggc gcctattcac
     3181 cggcacccgt agttaccccg gaggtacatg cgcgggtaat ggagcagatt atcctgccca
     3241 ccctgcgcgg catggccttt gagggcaatg cctacaccgg ctttttatat gctggcctga
     3301 tgatcgacgg cgcgggcaac cccaaggtga tcgaatacaa ctgccgattt ggcgacccgg
     3361 aaacccaacc tattatgctg cgcctgcagt ctgatctggt ggcactttgc caagccgcct
     3421 tagccggaaa tcttgctcaa accgacgctc aatgggaccc gcgcccagcg gtgggcgtgg
     3481 tactggccgc tggcggctac cccaatgact atgccaaagg cgatgtaatt gaaggcctgg
     3541 aagcggccgc cgcgctaccc ggcaaagtct ttcacgccgg cacccaacaa aaggacacgg
     3601 cacaaggcca ggcaattgtt accgccggag gccgagtcct ttgcgccacc gccttgggcg
     3661 agagcgtcac cctagcccaa caggccgcct accgtttggc agatgccatc agctggcccg
     3721 gctgcaccaa gcgccgggac attgcctacc gcgccatagc gcgggaatcc gcccggggct
     3781 gaacctagcg aaatctggca aggctgagcc ttgccgctaa aacctcagcc ggaaagtgcg
     3841 acttaaggct gaggtttttg ggctgggcat ctggccaaat cccggcaagc cagttaggct
     3901 tgccccccgc cccgttctgc cttccaaacc ttcggatcga atatgactga cgatactgcc
     3961 gctgaaactc aagtcttggt gctggccgct gaaagctccc gcaaacggtt taggctggag
     4021 aacgacatgc tggtggggcg cgaacccagc tgcgatatcc ccatcaactc cttgcacata
     4081 tcccggcagc acgcccgcat cacccaaaca ccgacaggct tttttttaga agacctcaac
     4141 tccgccaacg gcacctacat caacggcctg aaactcacca ccccagtggc cctacagatt
     4201 ggcgacctgg tgtgttttga cgatcagggc tttcgggtag tcgcggaagg aaaggttgaa
     4261 c
//
LOCUS       DAOQGS010000360         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11195_length_4256_cov_3.875506, whole genome shotgun sequence.
ACCESSION   DAOQGS010000360 DAOQGS010000000
VERSION     DAOQGS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11195_length_4256_cov_3.875506"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..3767)
                     /locus_tag="PKZ52_12865"
     CDS             complement(30..3767)
                     /locus_tag="PKZ52_12865"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG60708.1"
                     /translation="MNCAGNRYLGSNWALQLLLAALLWLSFSAAKLAALPSGAEVVAG
                     EASISQQGPVLQVEQSSQRAIINYQDFTIAAQETVNFNQPNAQAVVLNRVVGGNLAEI
                     YGHMNANGQVYLINPNGVLVGQGAQLNVGGLLLTTLDIAQEDFLAGRDALAGASSGRV
                     ENQGAIDASGQVILVAPEVINGGSIRAPHGEVALQAANRALLQTPGSGIPILVDEAQV
                     SGQVINTGRIEAVAVRLGSAGDYGPEANPAISNTGVVRAVRASGAGGVIELLAPGGEI
                     ANSGSLDASASHGAGGAVTAQAEYIANSGHVSASAAGGAGDGGAINLQASDSLALFGA
                     SQLSADGAGSGKGGEVLVVAEKDAWFTTTASISARSGEISGDGGFVEFSGHRSVDVNG
                     WVDVNAVNGKAGIWYIDPTDIVIANLDSGGSFTGGNPHTWVLSGPPATSRINVVSIRN
                     ALTSGDVIISTASGAASVGNITWNGNLDVNGLGTIRTLSLTANGTVSFQSGSIWDSNL
                     ATATDGLNINVTAGTGITVTDGVGINSTIGKINFIATTGDATITGLGSTANAADAIQV
                     TASAGRIVDGGDVYYDTTALVGNGGVVLSARDGIDIETNANTLSLLSSNGNVSVVNLG
                     AASVSQLSALTTSYLAASSIQTNNATVFGGSNVQLYATGNIVLPSNGLTVTGNLTLQG
                     NDIVDQNASRTLSVGANHLTVNSSAAGGNSIFNTAVNQLSVSNVSTNSLTFNDVDALT
                     TVSISPFSGTTTINSGAGTDLTVGSQIELNDETGALTFNAGKNLIVNGAVADSIGAIS
                     NNNALSFTAANNIIFNAGAQANAGAGSVALSVTNGNITLGGPIAGATMTVSAAGAAGG
                     NITSNASMDLNGFGGVRTLNLNASGVISFTGGTIADSDTSSASDGLNINATAGTGFSV
                     SGNTLLSAGAGKINITATLGNASVTGLSSSANAADAIRITASGGSIIDGGDSRTDISA
                     TSGGAILSGQFGITALETDVSVLDALSAAGDISLTEAGGLSISRLSALDSSSLTAGGA
                     ILTTNSTVFGGNDLNLITSSYIQVPQLGISMPDLVLLQAADVRAESAVRTVSLSGQHV
                     FVDTASSGGNLQINSTTNLLTLKNTGSNTITLNDTDALLLASVRPQGGSTVISSGAGS
                     NLTLANAPIELDGSNGVLTLNAGADLIINQSAEDTIGAVNNATTINLNATNNVTFTSG
                     GRVNAGTGTVAINASGGSVALGRVDAASTTVTAAPSYFASE"
     gene            complement(3748..>4256)
                     /locus_tag="PKZ52_12870"
     CDS             complement(3748..>4256)
                     /locus_tag="PKZ52_12870"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNG60709.1"
                     /translation="YGQGQVERLPDSNFNKLDLNGLVSQGFGSGRWRQVLQLTGRAQA
                     SDVSLPSIEAMALSGAYGVRGYEAGLFNAEQAALISLEWRLPNLVTGANWRLEPYLLA
                     EAALGRDLSYQGRSRSAHLDDVGLGLSASLGRHLRLNLLAVAGLGGALDGVQLDHEQQ
                     VLCELRWQ"
BASE COUNT          808 a         1323 c         1259 g          866 t
ORIGIN      
        1 agtaaattaa atggtaaaat tcctgttatt tattcagatg caaaatatga aggggctgct
       61 gtgactgttg tactcgccgc atcgacccgg cccagggcca cactgccgcc gctggcgttg
      121 atggcaacag tgccggtgcc tgcgtttacc cggccacctg aggtaaaggt gacgttattg
      181 gtggcgttaa ggttaatggt ggtggcgttg ttcacggcgc cgatggtgtc ctcggcgctt
      241 tggttgatga tcaaatccgc cccggcgttg agggttaaca cgccgttgga gccatccagc
      301 tcgatgggcg cattggcgag ggtgaggttt gaacctgccc cggaggagat caccgtactg
      361 cctccctgag gccgcaccga ggcgagtaac agcgcatctg tatcattcag ggtaatggta
      421 ttgctgccgg tgtttttcag ggtgagcagg ttggtggtgg aattgatctg caagttgccg
      481 ccgctgctgg cggtatccac aaacacatgc tggccgctaa ggcttaccgt gcgcacagcg
      541 ctttccgccc gcacatccgc cgcctgcagc aggactaagt ccggcatact gatgccgagc
      601 tggggcacct ggatgtaact gctggtgatc aggttgaggt cgttgccgcc aaacactgtg
      661 ctgttggtgg tgaggatcgc cccgcccgcc gttaggctgc tgctatccag ggcgcttaag
      721 cggctgatgc tgagcccgcc cgcctcggtc aggctgatat cccccgccgc actcagggca
      781 tctaacacac tcacatcggt ttctagggcg gtaatgccaa actggccgga gagaatggca
      841 cccccactgg tagcgctgat gtcggtgcgg ctatcgccac cgtcgataat actgccgcca
      901 ctggcggtta tccgtatggc gtccgccgcg ttggcgctgg aactcaaacc ggtcaccgag
      961 gcattgccca gggtggcggt gatgttaatt ttgcccgccc ccgccgacag cagggtgtta
     1021 ccggagacgc taaagccggt acccgccgtg gcattgatgt tgaggccgtc gctggcgctg
     1081 ctggtgtcgg agtcggcgat ggtgccgccg gtaaaactaa tcaccccgct ggcattaagg
     1141 ttaagggtgc gcacaccgcc aaagccgttg aggtccatgg aggcgttgga ggtgatattg
     1201 ccgcctgcgg caccggcggc gctcaccgtc atggtggcgc cagcaattgg cccacctaag
     1261 gtgatattgc cgttggtaac gcttaaggca acgctgcccg cccccgcatt ggcctgggcg
     1321 cccgcgttaa aaataatgtt gttggcggcg gtgaagctca gggcgttgtt attggagatg
     1381 gcaccaatgc tatcggccac cgcaccgttc acaatcaagt tcttaccggc gttaaaggta
     1441 agggcccccg tttcgtcgtt taactcaatt tgtgacccca ctgtcaaatc cgtcccagcg
     1501 cccgaattga ttgtggtagt gccggagaag gggcttattg agactgtggt aagggcgtct
     1561 acatcgttaa aggtcaggct gttggtggat acattggaca cgctcagctg gttaacggcg
     1621 gtgttaaaaa tcgaattacc gcctgcggca gagctgttaa ccgttaggtg gttggcgcct
     1681 acagagaggg tgcgggaggc gttttggtcc actatgtcgt tgccctgcag ggttaaattg
     1741 ccggtaacgg ttaagccgtt ggaaggcaac acaatattgc ccgtggcgta aagctgcaca
     1801 ttggaaccgc caaataccgt ggcgttattg gtttgaatgg aacttgccgc cagataactt
     1861 gtggttaacg ccgatagctg ggagacgctg gcggcaccga ggttgaccac cgatacattg
     1921 ccattggaac tgaggaggct cagggtgttg gcattggttt ctatatcaat gccgtcccgg
     1981 gcggacagca ccacgccgcc gttacccacc agggcggtgg tgtcgtagta aacatccccg
     2041 ccatccacaa tgcgcccggc gcttgccgtc acttggatcg catctgccgc attggcggtg
     2101 gagccgaggc ccgtgatggt ggcatcgccc gtggtggcga tgaaattaat cttgccgatg
     2161 gtggaattaa tgcccacgcc gtcggtaacc gtaatgccgg tgcccgccgt gacattaatg
     2221 ttcaggccgt cggtagcagt ggcgaggttg gaatcccaaa tactgccgct ttggaaactc
     2281 accgtgccgt tagcggtgag gctaagggtg cggatggtgc ccaggccgtt gacatccaag
     2341 ttgccgttcc aggtgatatt gcccacactg gccgccccgg aggccgtact gataattaca
     2401 tccccggagg tcagcgcatt cctgatgctc accacattga tgcgcgaggt ggcagggggg
     2461 ccggaaagca cccaggtatg gggattgccg ccggtaaacg agccgccgga atccagattg
     2521 gcaatgacta tatcggtggg gtctatatac caaatacccg ccttgccatt gaccgcattg
     2581 acatccaccc agccgtttac atctaccgag cgatggccgg agaactccac aaaaccgccg
     2641 tcgccgctga tctcaccgct gcgggcgctg atgctggcgg tagtggtaaa ccaggcgtct
     2701 ttttccgcca ccactaacac ttcgccaccc ttgccgctgc ctgcaccatc ggcactcaac
     2761 tggctggcac caaagagcgc cagggaatcg ctcgcctgca ggttaatggc gccgccatcc
     2821 ccggcgccac ccgccgccga ggcactgacg tggccgctgt tggctatgta ctcagcctgc
     2881 gcggtcaccg cgccaccagc gccatggctg gcgctagcat ccaggctgcc gctgttggct
     2941 atctcgcccc ccggcgctaa gagttctata accccgcctg cgccgctggc ccgcaccgcc
     3001 cgcaccaccc cggtgttact gatggcagga ttagcctccg ggccgtaatc gcccgcagag
     3061 cccagccgca ccgccaccgc ttcaatgcgg cctgtattga tgacctggcc actcacctgg
     3121 gcctcatcca ccaaaatagg aatgccgctg ccaggggttt gcagcagtgc ccggttcgcc
     3181 gcctgcaggg ccacctcccc gtggggggcg cggatgctgc cgccgttaat cacctccggc
     3241 gccaccaaaa tcacctgccc gctggcgtca atggcgccct ggttctccac ccggccactg
     3301 ctcgccccgg ccagcgcatc ccgcccggcg agaaaatcct cctgggctat atccaaggtg
     3361 gtaagcagca gaccgcccac attcagctgc gccccctggc ccaccaacac gccgttaggg
     3421 ttgatgaggt acacctggcc gttggcgttc atatggccgt aaatttccgc caggttgccg
     3481 cccaccaccc ggtttaacac caccgcctgg gcgttgggct gattaaaatt caccgtctct
     3541 tgggcggcta tagtgaagtc ttgataattg ataatggccc gctggctgct ttgctccacc
     3601 tgcagtaccg gcccctgctg gctgatcgaa gcctccccgg ccaccacttc ggccccgctg
     3661 ggcagggcgg caagcttagc ggcgctaaaa ctcagccaca gtagcgccgc cagcaatagc
     3721 tggagtgccc agttagagcc taagtaccta ttgccagcgc aattcacaca gcacctgctg
     3781 ttcatgatca agttgtactc catccagcgc gccacccagg cccgccaccg caagcaagtt
     3841 caggcgcaga tgccgcccca ggctggcact aagccccagc cccacatcat ctaaatgagc
     3901 gctgcggctg cggccttggt agctgaggtc gcggccgaga gccgcctccg ccagcaggta
     3961 gggctccaac cgccaattgg cgcccgtgac taaattcggc aaacgccact ccaggcttat
     4021 aagcgccgcc tgctcggcgt taaacagccc cgcttcataa ccgcgcacgc cgtaggcacc
     4081 cgagagcgcc atggcctcaa tactcggcag gctcacatcg ctggcctggg cgcgccctgt
     4141 cagctgcaac acctggcgcc agcggccgct gccaaagcct tggctgacca ggccgtttag
     4201 atccagcttg ttgaagttgc tgtccggcag ccgctccacc tggccctgcc catagc
//
LOCUS       DAOQGS010000361         4254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11211_length_4254_cov_4.492498, whole genome shotgun sequence.
ACCESSION   DAOQGS010000361 DAOQGS010000000
VERSION     DAOQGS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_4254_cov_4.492498"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4223)
                     /locus_tag="PKZ52_12875"
     CDS             complement(<1..4223)
                     /locus_tag="PKZ52_12875"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG60710.1"
                     /translation="MPYLSNIRRWIVSAVGGALYVYAFVGWASPEGGHIVAGQGVIAQ
                     AEKETRIQQASDRLSIDWKGFDINSDERVQFIQPNSSSIAFNRILSNKGSLIQGRIDA
                     NGQVVLINPHGLIFTEGASVNAGGLIASALQMSDQDYLNGSFTLNALEGSEGRVINSG
                     LLNAATGGTISLLGQSVENKGLISAQLGSVNLAAGKEAVLTFGPNGLVGVRVTQAVLQ
                     KELGVDAAIVNSGEINAEGGKVLITASTSKDIFSKAVNVGELSPAKSAVVNDDGSFTL
                     RTGADIKNTGAINVGADAKAGEVVLTGENISNQGSINADSQTTHAGSVEITAKNINET
                     KDNGTVTAVAHNSGKGGDIKLLGDKVGLLDNANIDVSGTNGGGQILVGGDKTGTNPFI
                     QNAQFVYVDEGAHIKSNAIERGDGGRLIAFAADSATLLGQLEAKGGHLIGNGGFIETS
                     GLYGLVLYGSPTITAANGLAGEWLIDPFDLSISDASTRRVSTDTQTFYTSSGTGARVF
                     WDALENVLSANGAIVTIRTGGATTSEPGNITFDYTGNNPSFNFVFDNVGNITSTLKLN
                     ADGDIKFSDKISGLTASVTNNNRLNLDFTAGGDIDLNKVAISTNGGYFTAAANGTQKL
                     SSNPDVFAAGDFKAVDASISTYGAFGGGDIRITTKGKADLGAMDFSYYYDNGNTHKLT
                     QVGSINVTAGGDIILNKNFDFNNSGGYAPDGTANNVTDTTALTLSADGSITLNGVIAD
                     KEYGDGRDALGINVEADHNKDGIGSVTINQPIYTAGGSIVVSGAGIIQNALINTDRAN
                     ERIVVEQANGKNGNATRSEGGNVTFSSDTDVTLNAITTDSFCTLGTCTGNWMVQSRTA
                     GTKINIAQADNTHLTIHGTTDFTINGGTINLGNRDNIFGTQGGSVEAQAGNAVSKTLT
                     LGTVGNTTVSGLGLLLGAADINGTARLTATSPTASVNAITQAPNTSLTVTGSTELTAN
                     TIALENNGNDFSIINSLDAATSVRVFDSANDLVLNTLKTPNLTLNTLGDAHFNGTIIT
                     NDKSVSITQNGAGAVALADSLTQAGTGAVTFNGTVLSAGFKLLSNQNAAWDLAAKTLV
                     WDSKTFGFEGFTRLIGGAGVDTVRLSTSVNAIDLDNQSSTGFVLSNMEVIKATAANAS
                     LKGFKANSQWTITGDKKGTIVGGGQTTAFEGFNELISSDDASNTDLFTFNSAGAIGKI
                     TGQNADVVNLFPGTDGAGVDYDWAVTANTLNGSTTASAQVKRSGQSTDVIAALTGVGT
                     INSSDGKDTVTASATYAGTIDLKAGVNALTTDSARLLFSSRPLNMNGFVGVNDLNAKT
                     QAATLTASTDDVSAILAWAVNPAEDALKGPLSGSITYAQSGQADQTLSFKGFATLTGG
                     ANNDYFTLNNVALNTGTFTGGQINAGTPA"
BASE COUNT         1065 a         1104 c          923 g         1162 t
ORIGIN      
        1 ctggcaggtg taccggcatt aatctgccca ccggtaaatg tgcctgtatt taacgcgaca
       61 ttatttaacg tgaaataatc gttattggcg ccgccggtta aggtagcaaa acctttaaag
      121 ctcagggttt gatctgcttg cccgctttga gcataggtaa ttgagccact taacgggcct
      181 ttaagcgcat cctccgctgg gttaacggcc caggcgagga tagcactgac atcatcggta
      241 cttgccgtta aggttgcagc ctgggttttg gcgttaaggt cattaacccc cacaaaccca
      301 ttcatattta aagggcggct ggaaaacaaa agacgcgcag aatcggtggt taacgcatta
      361 acgcccgctt ttaaatcaat agtgcctgca taggtcgctg acgcagtaac cgtatccttg
      421 ccatcactac tgttgatagt gcccacaccg gtaagcgctg caataacatc cgtactttga
      481 ccgctgcgtt ttacctgggc cgaagccgtg gtactaccat tcagggtgtt agccgtaacg
      541 gcccagtcat aatcgacccc ggcgccatct gtgcccggaa acaaattaac cacatcagcg
      601 ttttggccgg taattttgcc aatggcgccc gcgctattaa acgtaaatag gtcggtattt
      661 gaagcgtcat ccgagcttat taattcatta aagccttcaa atgcggtggt ttggccaccg
      721 ccgacaatag tgcctttttt atccccggta attgtccatt gggaattggc tttaaaccct
      781 ttgaggcttg cgtttgccgc cgtggcttta atcacttcca tattcgataa cacaaaaccg
      841 gtggatgatt gattgtctaa atcaatggca ttaacactcg tcgataaccg caccgtgtcc
      901 acacctgcac caccgataag gcgcgtaaac ccctcaaaac caaacgtctt gctatcccac
      961 actaaggttt ttgcggctaa atcccaggct gcattttgat tgcttaacaa tttaaaccct
     1021 gcacttaaca cagtgccatt aaatgttacg gcgccagtac ccgcttgagt gaggctatcc
     1081 gcaagcgcaa ccgctccagc gccattttgt gtaatcgaga ctgatttatc gttggtaatg
     1141 atagtgccgt taaagtgagc atcgcccagg gtattgagcg ttaaattcgg tgttttaagc
     1201 gtgtttaaca ccaggtcatt agcactatca aacacgcgga cagaggttgc cgcatcgagg
     1261 gaattaataa tggaaaagtc attgccgttg ttttcgaggg caatggtgtt agccgttaat
     1321 tctgtgctgc cggtaacggt taaggacgta ttcggtgcct gcgtaatagc attaaccgag
     1381 gccgttgggc tggtggccgt caaccgtgcc gtaccattaa tatcggcagc ccccagcaat
     1441 aaacccaatc cgcttactgt ggtattgcca actgtaccga gcgtaagggt tttagaaacc
     1501 gcattacctg cctgcgcctc aacactgcca ccttgagtgc caaaaatatt atccctattg
     1561 cctaaattaa tcgtgccacc attaatagtg aaatccgtag tgccgtggat ggttaaatgg
     1621 gtgttatccg cttgggcaat attaattttt gtacccgccg tgcggctttg caccatccaa
     1681 ttaccggtgc aggtgcccag cgtacaaaat gaatccgtgg tgatggcatt taacgtcaca
     1741 tcagtatcgc tgctgaacgt aacattgccg ccctctgagc gggtagcgtt gccatttttg
     1801 ccgttggctt gctcaaccac tatgcgctca ttggcgcggt cggtattaat cagcgcgttt
     1861 tgaataatac ccgcaccgga aaccacgata gaaccacccg ccgtataaat gggttggtta
     1921 atcgtgacgc tgcctatacc gtctttatta tggtcagcct ctacattaat tcctaacgca
     1981 tcgcgcccgt cgccatattc tttatcggct atgacaccat ttaaggtaat ggagccatcc
     2041 gcactcaagg taagtgctgt cgtgtcagta acattattgg cggtgccgtc cggcgcataa
     2101 ccgcctgaat tattaaaatc aaaattttta ttgagaataa tatccccgcc tgcggtgaca
     2161 ttaatcgaac ctacctgggt caatttatgc gtattaccat tatcgtaata atagctaaaa
     2221 tccatggccc ctaaatccgc tttgcccttg gtggttatgc gaatatcacc gccaccgaat
     2281 gcgccatagg tgctaatgct ggcatcaacc gctttaaaat caccggcggc aaacacatcc
     2341 gggttactgg ataacttttg ggtgccattg gcggcggcgg tgaaatagcc accattggtt
     2401 gaaatagcca ctttgtttaa atctatgtcg ccacctgcgg tgaagtctag attgaggcga
     2461 ttattattag tgaccgaggc cgttaagccg ctaattttat cggagaattt tatatcgcca
     2521 tcggcattga gttttagcgt agaggtgata ttaccgacgt tatcaaagac gaaattaaaa
     2581 ctggggttgt tgccggtgta atcaaaagta atattgccag gctcagaagt agttgcacct
     2641 ccagttctaa tagtcacaat agcgccattt gctgaaagca cattttcaag agcatcccag
     2701 aaaaccctag ctccagtgcc gctagaggta taaaaagttt gagtgtcagt actaactcgt
     2761 cgagttgagg catcacttat ggataagtca aatggatcaa ttaaccattc acctgcaagg
     2821 ccatttgctg ctgtgattgt tggactgcca tacaaaacta atccatacaa acccgacgtt
     2881 tctataaatc caccattgcc aattaaatgc ccacctttag cttctagttg ccccaataga
     2941 gtggctgagt ctgcggcaaa ggcaatcagc ctaccgccat caccacgctc tattgcattg
     3001 gattttatat gtgccccttc atcaacatat acaaactgtg cattttgaat aaatggattg
     3061 gtgcccgttt tatcaccgcc cactaaaatc tgcccgccac catttgtacc cgagacatct
     3121 atgttggcgt tatccaacaa accgactttg tcgcctagta atttaatatc cccgcctttg
     3181 ccactgttgt gcgcaacggc ggttactgtg ccattgtctt ttgtttcgtt aatatttttt
     3241 gcagtgattt caacactgcc tgcatgggtg gtttggctgt cggcattgat actaccttgg
     3301 ttagaaatgt tttccccagt cagtacgact tcaccggctt tagcgtctgc acctacattt
     3361 attgccccgg tattttttat atccgcacca gtcctcaagg taaaactgcc atcatcgttc
     3421 accacggcac ttttggcagg cgataactcc cctacattca cagctttgct aaatatatct
     3481 ttgctggtgc tggccgtaat tagaacctta ccgccttcgg cattgatctc cccgctattc
     3541 acaatggcgg catccacacc caactctttt tgcagcaccg cctgggttac ccgtacaccc
     3601 accaatccat tcggcccaaa ggttaaaacc gcttccttac cagcggctaa attaacactg
     3661 cccaactggg cgctaatcag gcctttattc tccacgcttt ggcccagcaa gctgatagtg
     3721 ccgcctgtgg cggcatttaa caggccggaa ttaataaccc ggccttcgct gccctctaag
     3781 gcatttaacg taaagctacc gtttaagtaa tcttggtcgc tcatctgcag ggcggaggcg
     3841 attaatccac ctgcattgac gctagcacct tcggtaaaaa taagcccatg ggggtttatt
     3901 aataccacct gcccattggc atctatacgc ccctgaatta acgagccttt attgcttaaa
     3961 atgcggttaa acgcgatgga ggaactattg ggctgaataa actgcacccg ctcatcgcta
     4021 tttatatcaa aacctttcca gtcaatactt aagcggtcac tggcttgctg gatgcgggtt
     4081 tctttttctg cctgcgctat aacaccctgc ccggctacga tatgcccgcc ctctgggctt
     4141 gcccagccta caaaggcata cacatacaac gcaccgccta cagcagaaac aatccaacgc
     4201 cggatattgg ataaataggg catacattcc gctccattgc acatcattaa tttt
//
LOCUS       DAOQGS010000362         4234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11307_length_4234_cov_4.478823, whole genome shotgun sequence.
ACCESSION   DAOQGS010000362 DAOQGS010000000
VERSION     DAOQGS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11307_length_4234_cov_4.478823"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..3531
                     /locus_tag="PKZ52_12880"
     CDS             100..3531
                     /locus_tag="PKZ52_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG60711.1"
                     /translation="MDREGQLWLAVQHGVFRFNPQTEQFKRYLNDPADRYSYSGKITR
                     DVFMDSQGILWIGTDEGGLCVYDYEKDRFYSFQHVEGRLGAISSNSIRRIYEDNSGDL
                     WVGTYPSGVNFHDRSTAAITVYTSDADPKAGLMGDTVEAVVEDHDGNIWIGSGGITKF
                     NRKDDTLTHYKKTGGADSRVNSTAIISGHVDSDNDIWFGTWAGGLQHYNPTTDRFDQI
                     PFDPTLAGNGQKTSTSLNDGSVWHIYEDKQKTLWIATHLGGLSKYDKKTKIFTNYTQV
                     VSDPTTLSNKLVWTMMEDSKGRFWVGTASGLNLMDREKETFKQYKADANNPAALVNNS
                     VLSIYEDKKGRLWFGTDGGLHLYNEQNDGFSVYNTKDGFVDNGIRSIIEDDANNLWLG
                     TNNGVVMFNPDTKIVKNYRRYNGEQIGGFATGAAIATSRGEMVFGGKNGLRFFDIKKL
                     ASNQKVPPVVVTDFRLFTKPVAVNDSDGLLSQVINQTRELTFDYKKTMFSFGFSALNF
                     RDPDKNQYAYKLEGFDDKWREVGNQRTALYTNISAGNYTFRVRASNNDGVWNETGRAI
                     AIRQLPPPWKTWWAYTIYVLIGLGIVVQFVLSQRKKRRLVEEQNRILEIRVAERTSEL
                     REKNNDIQAMLSNMRQGLFTIEASGNIHQEYSLFLEEIYETKEIAGRNVMDLLFGNAQ
                     LGGDTFDQIKEAIGSIIGEGEMSFDFNSHLLLKEYQADIDGNTKHLSLDWSPIVADDI
                     IAKLMVSVRDVTQLKQMEAEAKSKKRELDIISQLLNVPAKKFLAFAESTKTFIAENRK
                     HIHATAAYSESVVALLFRNMHTIKGNCRTFAFGYFSDIVHEVETVYSTLKNAVDETPW
                     EQEKLLADLASVDTILAEYERVFYTVLGRGDGTTGARDQNGFWADSKAMQTIQTCIDK
                     VAQDFPAVGKAQYFAPVLSMLEKAQSNTLDEVLADIVDSLGSIATQLDKERPLVKIDA
                     ENVRIKTSSNELLTNVFAHILRNSVDHGIESPADRVQKGKAERGTISISAEAVDGHLD
                     IHVRDDGQGINVDKLFQKGVETGRWKEGEKPTYQEIAQLIFVSGISTKDQVSDISGRG
                     VGMDAAMQFLLEHGGAISLNLADTQASGPAQIGTGVKIPFELVVTLPAPAFLQAA"
BASE COUNT         1256 a          882 c          990 g         1106 t
ORIGIN      
        1 ctgtcgcggt tagactgggc taccaaaaaa gtcagcacct atatcccaga ccccaaaaat
       61 cctggctcgc aaactgataa cgcagtttgg cgtatccaag tggaccgtga gggccaattg
      121 tggttggctg tacaacatgg tgttttccgt tttaatccgc aaactgaaca atttaagcgg
      181 tatctgaacg atccagcgga tcgctatagt tatagcggca aaataactcg tgatgtgttt
      241 atggatagcc aaggaatttt atggataggg acagatgaag gcggcttgtg tgtatatgat
      301 tatgaaaaag atcgctttta cagctttcag catgtcgaag gtcggttagg cgccatcagc
      361 tcaaactcga ttcgtcgtat ctacgaggat aatagtgggg atttatgggt gggcacctat
      421 ccttcggggg tcaattttca tgatcgatct accgccgcaa ttacagtcta taccagcgac
      481 gccgacccta aagctggcct tatgggcgat accgtagagg cagtggtgga agatcacgac
      541 ggcaatattt ggatcggctc cggtggtatt acaaaattca accgcaaaga cgatacctta
      601 acccattaca aaaaaaccgg cggtgccgac tcgcgcgtta attccactgc gattatttcc
      661 ggccatgtcg attctgataa cgatatttgg tttggtacct gggccggggg attgcaacac
      721 tacaacccaa cgaccgaccg ttttgatcaa atacccttcg atccaacctt agctggtaat
      781 ggccaaaaaa cctcaaccag tcttaacgat ggtagcgttt ggcatattta tgaagataaa
      841 caaaaaacct tatggattgc cacacatttg ggcggcttaa gtaaatacga caaaaaaacc
      901 aagatattta ccaattacac tcaagtcgtt tctgacccga ctactctatc caataaactg
      961 gtatggacca tgatggagga ttccaaaggg cgtttttggg taggtacggc cagtgggtta
     1021 aacctgatgg atcgagaaaa agagacattt aagcaatata aagccgatgc aaataatcct
     1081 gcggctttag ttaataattc ggttttatct atttacgaag ataaaaaggg caggctgtgg
     1141 tttggcacag atggtgggct tcatctgtac aatgaacaaa acgatggctt ttctgtatac
     1201 aacaccaagg acggatttgt tgataatggt attcgcagta ttattgagga tgatgccaat
     1261 aatttatggc ttggaaccaa caatggcgta gtgatgttta atcctgacac aaagattgtt
     1321 aagaattatc gccgctataa cggcgagcaa attggtgggt tcgctaccgg cgctgcaatt
     1381 gcaactagcc gaggcgaaat ggtctttggt ggtaagaatg gcttacgctt ttttgatatc
     1441 aagaaattag cctccaatca aaaggtaccg ccagtggtag taaccgattt tcggctattc
     1501 accaaacccg tggcagttaa tgactctgat gggttattga gccaggtgat taatcaaacc
     1561 cgcgaactta ccttcgatta taagaaaacc atgttttcgt ttggattttc tgcgctaaat
     1621 ttccgcgacc cggataaaaa tcagtatgcg tataaattag aaggattcga cgataagtgg
     1681 cgggaagtgg gcaatcaacg cacggcgctt tataccaata ttagtgcagg taattacacc
     1741 ttcagggtga gggctagcaa caatgatgga gtctggaatg aaactggcag agcaatcgct
     1801 atccggcaat tgccaccgcc ctggaaaacc tggtgggcct ataccatcta tgttttgata
     1861 ggccttggga ttgttgtgca gttcgtattg tcgcaacgca agaaacgccg tcttgttgaa
     1921 gagcaaaacc gtatactgga aattcgtgtg gccgagcgca ccagcgaatt gcgagagaaa
     1981 aataacgata ttcaagctat gttgagcaat atgcgccagg gtttgtttac cattgaggct
     2041 agcggcaata tccatcaaga atactcccta tttttggagg aaatatatga aaccaaagag
     2101 atcgctgggc gcaacgtgat ggatttgtta tttggaaatg cccagcttgg tggcgatact
     2161 tttgaccaaa ttaaagaggc cattggttca ataattggcg aaggtgaaat gagttttgac
     2221 ttcaactctc acctgctgct taaggaatac caagcggata ttgatggcaa cactaaacat
     2281 ctatccctcg actggagccc tatagtcgcc gacgatatta tcgccaaatt aatggtatcc
     2341 gtgcgcgatg ttactcaatt aaaacaaatg gaagctgaag caaaaagcaa aaaacgcgag
     2401 ctggatatta tcagtcagct actaaatgtt cctgccaaaa aattcttggc atttgcagaa
     2461 tcgaccaaaa cctttattgc cgaaaatcgc aaacatattc atgcaacagc agcttacagc
     2521 gaaagtgttg tagcgctact cttccgtaat atgcatacca ttaaaggcaa ctgccgtacg
     2581 ttcgcttttg gatactttag cgatattgtg cacgaagtag agacggttta tagcactctt
     2641 aaaaacgccg ttgacgaaac cccatgggag caagaaaaat tgctggcgga cctagcatcg
     2701 gttgacacca tattagcgga atatgagcga gttttttaca ccgtattggg gcgcggcgat
     2761 ggcactaccg gagcacgaga tcagaacggc ttttgggcgg atagcaaagc gatgcagact
     2821 attcaaacct gtatcgacaa ggtcgcacag gattttcctg ctgtcggcaa agcgcaatat
     2881 ttcgcaccgg ttctaagcat gttagaaaaa gcacagtcga atactctaga tgaagtgctg
     2941 gccgatatag ttgattcctt aggttcaatt gccacacagc tcgataaaga gcggccccta
     3001 gtaaaaattg atgcagaaaa cgtgcgtata aaaaccagtt ccaatgaatt attaaccaat
     3061 gtttttgcgc acattttgcg caattcagtg gatcacggca tagaatcacc tgcggaccgc
     3121 gtgcaaaaag gcaaagcaga acgtgggaca attagcatat cagcggaagc cgtagacggg
     3181 catctggata tccatgtacg tgacgatggt caaggtatta atgtcgataa gttattccaa
     3241 aaaggtgtgg aaaccggtcg ctggaaagaa ggtgaaaagc caacatatca ggaaattgct
     3301 caattaattt ttgtgtcggg catttccacc aaagaccaag tgagtgatat atcaggtaga
     3361 ggggtcggca tggatgctgc catgcaattt ttattggagc atggtggcgc aatctcgttg
     3421 aatttagccg atactcaagc ctcggggcct gcgcaaattg gcactggagt taaaattcct
     3481 tttgaattag tggtaacctt acccgcaccc gcattcttgc aagccgctta atttgaggtt
     3541 gcatttttta aaccaagggg ctctcctctt ggttttttat tgtttgactc gttttacgcc
     3601 tacgcctaga agtttattat tctgcgagaa atttaaagac cctatgatta ctgccacttt
     3661 caatgccaga actggcaaaa aacttccaga ggtttagaga ataccactta ccagaaaatt
     3721 cctagcagcc ttttgaacac acaggcgggg cactctaaca actaatattt ggctaaaaag
     3781 cgtattttac acctaataaa tgagtatttt acggggaggt ctagcccaac tttagcaccg
     3841 caggaggtag caaggtagta tttcagcaag cggctcgggc aatgatcaat aaagtaagcg
     3901 acgttccaac ttaatgtagt gcactatgtt aataatagtg cgggatttag tgccgtgttg
     3961 atagtggtgg caaggaaacc acgtctagca gcttgttgaa atactacctt gccagcgagc
     4021 gcctacgcat ttaaaaaagc cgtaacccat tatattctgt gcgttttcgc aagctttagc
     4081 gcagtgacga aaaacaaatc agtaaaccgc ttttgggcac cgatttaata aaaatttccg
     4141 ctaaggtata gattgagcag tgatgctcaa cacttaaaaa cctcctaata tctaattgga
     4201 acactacgct atccaatatc ggtggactcg taac
//
LOCUS       DAOQGS010000363         4214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11382_length_4214_cov_4.906708, whole genome shotgun sequence.
ACCESSION   DAOQGS010000363 DAOQGS010000000
VERSION     DAOQGS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11382_length_4214_cov_4.906708"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1049)
                     /locus_tag="PKZ52_12885"
     CDS             complement(150..1049)
                     /locus_tag="PKZ52_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNG60712.1"
                     /translation="MDMLWYVVGGLALWLLAGVRIVEQQSVVIVQFLGRYSCTLEAGI
                     NWIFVPFQLNAGRLSLKIESVPATVEVKTSDNMFVSLPVNLMIQVEPQNAKDAFYKLQ
                     KPHEQVRAWVLNTVRSIAAGMTLEEMFNDRERVVNEVRALLTAKLSEFGYRLEGVLID
                     QPMVSAEVQSSFNRVVAATREKEAALQEGEAIKIRIVKQAEAEADAQRERAKGLADSR
                     RILAVGLQESLNGFAKIPQNTALALLIETNRIDALREASKHGNLILMDLGKADGAQKA
                     LIPLLKGLKNADAPVASSADESN"
     gene            complement(1102..1608)
                     /locus_tag="PKZ52_12890"
     CDS             complement(1102..1608)
                     /locus_tag="PKZ52_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNG60713.1"
                     /translation="MPRKFIKRWLPDADTLRKTPGLKSLGHVLDDPHLFHLSRRSVAV
                     AFLVGLFFCYIPTPGQAFLAAFGALVLRGNLPIAVALVWVSNPFTLPFFVLSAYGAGA
                     FVLGGGAIELPESITWAWFKTIWQPFLLGSVIMGLVAGLAGYGAINLIWRVSVARRWV
                     RRMRERAQ"
     gene            complement(1643..2611)
                     /locus_tag="PKZ52_12895"
     CDS             complement(1643..2611)
                     /locus_tag="PKZ52_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNG60714.1"
                     /translation="MIRAIVLLGDGRIKHGGQELVAYWQAHGDSFIWLDMQGETAEDE
                     SQMLRELNCHPLAIEDVQRFRHPPKTETFAQHTLVLYRGITQFNDDLTIEQQTIALFA
                     MERGLISVHAKPSTAIDFWWDEVVPAGLLRSPGFLACRIMHYSVGRYVDKVLEFEPRL
                     TELEDSMQDHPSDDVMKELIAYKSRLRKLKRIFSYHEKLVSNLLKNTPIKLVEEEGEI
                     EHALQDLYERCERLTSLTTMYFDLCGDLIEGYLSISSHNLNKTMQFLTVITAILGPLT
                     IVVGVYGMNFDVMPELHWRFGYAFVWGLMLSIVGFSFWFFRRRRWL"
     gene            complement(3034..3327)
                     /locus_tag="PKZ52_12900"
     CDS             complement(3034..3327)
                     /locus_tag="PKZ52_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60715.1"
                     /translation="MKNLIAIIVIAASFVSGVCRAELKPGATELNSVEKRLDLLIKQN
                     QSLIAENSALKERCDKPKTKEEVFAACMQAAKGGNSAMAAESIGIRCEQLLKN"
     gene            complement(3403..4143)
                     /locus_tag="PKZ52_12905"
     CDS             complement(3403..4143)
                     /locus_tag="PKZ52_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60716.1"
                     /translation="MAILTLSAEAPLLARWAADAILFLHIAGGGLGILAGSIVLLLPK
                     GGRPHRIVGTVFFIAMGVAFGIGACVAPFLDTGQRPNFIAGVFAFYLLVSAWLTIKYS
                     HVNLALHSVGIVIALSTALAGGVFIYLANNNATGTIDGAPPQSFYVFLILGAAATMDD
                     FMVLLRRTSSCQARLARHLWRMCAAVFFASGSLFLGQPQVFPDYLRGSPVLFMPVLLP
                     LLVMVFWLLRISLRLWRARTAAIVPAKP"
BASE COUNT         1176 a         1192 c          930 g          916 t
ORIGIN      
        1 cctgccattg gacgccaccg ccgcttttgt gggctacaac attttggcca atagcctggg
       61 taaagcgcag cttaccgccc gctacggacg ctaaaaaacc acgctaaaaa gcccctcgcg
      121 gggctttttt atgccggtat ttttatctat taattgctct catccgcact ggatgccact
      181 ggcgcatccg cattttttaa gcccttcagc agtggaatta acgccttctg cgcaccgtca
      241 gccttaccca aatccatcaa aattaaattg ccgtgtttgg atgcctcccg cagtgcatcg
      301 atgcggttgg tttcaattaa tagggctaag gcggtatttt gcggaatttt tgcaaagcca
      361 tttaaggact cttgcaaacc cacggctaaa attcgccgcg aatccgccaa gcctttagcg
      421 cgctcgcgct gggcatccgc ttccgcttcc gcctgtttta ctatgcggat tttaatcgcc
      481 tcgccttctt gcaacgccgc ttctttttcc cgcgtggcgg ccaccacgcg gttaaacgaa
      541 gactgcacct ccgccgacac catgggctga tcgattaata caccttccag gcgataacca
      601 aattcggata attttgcggt taacagcgcg cgcacttcat tgaccacccg ctcgcgatcg
      661 ttaaacattt cttctaaggt catgcccgcc gcaatggagc gcaccgtgtt aagcacccaa
      721 gcgcgcacct gctcgtgggg tttttgcaat ttataaaaag catctttagc attttgcggc
      781 tccacctgaa tcattaaatt caccggcaaa gacacaaaca tattatcgga ggtttttacc
      841 tccacggttg cgggcaccga ttcaattttg agggaaagcc tgcctgcgtt taattgaaac
      901 ggcacaaaaa tccaattaat gcctgcttcc aatgtgcagg aataacgccc caaaaattgc
      961 acaattacca ccgattgctg ctcgacaatg cgcacccctg ccagcagcca cagcgccaaa
     1021 ccgcccacca cataccaaag catatccata attaccctct agtctttttt gttgttgata
     1081 atttattctg gccgcacaga cttattgcgc ccgctcacgc atacgccgca cccaacgccg
     1141 cgccacgcta acgcgccaga tgagattaat cgccccatag cccgccaaac cagccacaag
     1201 ccccataatg acactgccca ataaaaaagg ctgccaaata gttttaaacc acgcccaggt
     1261 aatggactcc ggcaattcga tggcgccacc acccaacaca aacgcccccg ctccataggc
     1321 acttaacaca aaaaacggca gggtaaaggg gttgctcacc cacaccagcg caaccgcaat
     1381 gggcaaattg ccgcgcaata ccaatgcgcc aaaggccgct aaaaaagcct ggccaggggt
     1441 tgggatataa caaaaaaata aacccactaa aaatgccacc gccaccgagc gacgacttaa
     1501 atgaaataaa tgcggatcat ccaacacatg gcctaaggat ttaaggcctg gcgttttacg
     1561 cagcgtgtct gcatcaggca accagcgttt aataaattta cgcggcatag attattccgg
     1621 ctcagcaagt ataaaagtgc atttacagcc atcgacgacg gcgaaaaaac caaaacgaaa
     1681 acccaacaat ggacaacatc aagccccaca caaaggcata accaaagcgc caatgtaatt
     1741 ccggcatcac gtcaaagttc ataccgtaca cccccaccac aatggttaaa gggcctaaaa
     1801 tggcggtaat caccgttaaa aactgcatgg ttttattaag attgtgggac gaaatcgaca
     1861 gataaccctc aattaaatcg ccacacaaat caaaatacat ggtggtaagg ctggtcagcc
     1921 gctcacagcg ctcatacaag tcttgcaagg cgtgctcaat ctcgccctcc tcttccacca
     1981 atttaatcgg tgtgtttttc aatagattgc tgaccagctt ttcatgatag ctaaaaattc
     2041 gcttcagctt gcgcaggcgg gatttataag caattaactc tttcatcaca tcgtcgctgg
     2101 ggtgatcctg catggaatcc tccagctccg tcaggcgcgg ctcaaactcc agcaccttat
     2161 ccacataccg gcccaccgaa taatgcatga tgcggcaggc caaaaaaccg gggctgcgca
     2221 aaagccccgc tggcaccacc tcatcccacc aaaaatcaat ggcggtggag ggcttggcat
     2281 gcacactaat cagcccccgc tccatggcaa acaacgcgat agtctgctgc tcaatagtga
     2341 gatcatcatt aaattgcgtg ataccgcgat agagcaccag cgtatgctgg gcaaaggttt
     2401 ccgtcttggg cgggtggcga aaacgctgta catcctcaat ggccaggggg tggcaattaa
     2461 gctcgcgcag catctggctc tcatcctcag ccgtctcacc ctgcatatcc aaccagataa
     2521 aactatcccc atgggcctgc caataggcca ccagctcctg ccccccatgc ttaatccgcc
     2581 cgtcccccaa caacacaatc gctctgatca cggctacctc agctcgcgct aaaaggccgg
     2641 gattatttca tactcaaggc ggtttagggc ggcccagagg gtggagtggt ctggccaaca
     2701 atctcattaa gttaaggaac aggggcgcac atttgaagta gtgctatctg ggtagcctgg
     2761 atggagcgaa gcgtaatcca ggctacagaa atggcaagta atcgtgaact taagaaaatt
     2821 gcctgaggag gaggccgcag aagtggccgg tggacatatc accaagttaa ggattacagc
     2881 actttattcg gtgtgaaatc gcccaggtaa cattgattct gccgcgcaac gtaagccggt
     2941 tgtgggacaa acccggctta cgctgccgca actggctagc agtctcgtta aacaaatccc
     3001 tcaaataaac caaaccgcta ttgaatcgct aaattaattt tttaacagtt gctcacagcg
     3061 gatgccaatg gattcagccg ccatagcact gttaccccct ttggccgctt gcatacaggc
     3121 agcaaatact tcttcttttg ttttaggttt atcgcagcgc tcttttaggg cgctattttc
     3181 cgcaatcagg ctttgatttt gtttgatcag aagatcaagg cgtttttcga cagagtttaa
     3241 ttccgtagcg ccgggtttaa gttctgccct acagacaccg gaaacaaaac tcgcggcaat
     3301 tacaataatg gcaattaaat ttttcatagc agatcctctt gggtgacgtt gaaaggtgga
     3361 gcggtaaaac aggcgttatg gccaatttag aacaaactta ggttagggct tcgcaggcac
     3421 aatcgctgcg gtgcgcgcac gccacagcct tagcgaaata cgcagcaacc aaaacaccat
     3481 aaccaacagc ggcaataaga ccggcataaa aagcactggt gagccgcgca aatagtcagg
     3541 aaatacctgg ggctggccga ggaaaagtga gcctgaggca aaaaataccg ctgcacacat
     3601 ccgccacaaa tgccgtgcta aacgtgcctg gcaggagcta gtgcggcgca agagaaccat
     3661 aaaatcatcc atagtggccg ccgcgcctaa aattaaaaac acataaaaag actgcggcgg
     3721 cgcgccatca atggtgccgg tggcgttatt gttagccaga tagataaaaa caccgcccgc
     3781 tagagctgtg ctcaacgcaa tcacaatacc cacactgtgc agcgctaaat ttacgtggct
     3841 gtatttaatc gtcagccaag cgctcaccag taaataaaat gcaaatacac cggcaataaa
     3901 atttggccgc tggcccgtat ctaaaaaggg cgcaacacag gcgccaatac caaatgccac
     3961 ccccatggca ataaaaaata cggtgccaac aatacggtgc gggcgcccgc ccttgggcag
     4021 cagcaatact atgctacccg ccagaatccc caggccaccg cctgcaatat gcaaaaataa
     4081 aatggcatcg gcggcccatc tggccaataa gggtgcctct gcactcaagg taagaattgc
     4141 catataagct cccgttgtta ctattaaccc ggcggctaaa tagatcgtcc tgatctattt
     4201 agccttcgct gccc
//
LOCUS       DAOQGS010000364         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11413_length_4208_cov_3.706959, whole genome shotgun sequence.
ACCESSION   DAOQGS010000364 DAOQGS010000000
VERSION     DAOQGS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11413_length_4208_cov_3.706959"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKZ52_12910"
     CDS             complement(<1..234)
                     /locus_tag="PKZ52_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNG60717.1"
                     /translation="MSLHIPGYRIIRTLGKGGMATVYLAVQDIFERQVALKVMAQGLT
                     DDPAFGKRFFREARIVSQLVHPNIVTVFDVGVHE"
     gene            complement(372..689)
                     /gene="grxD"
                     /locus_tag="PKZ52_12915"
     CDS             complement(372..689)
                     /gene="grxD"
                     /locus_tag="PKZ52_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNG60718.1"
                     /translation="MDIIETIKEQIAQNPVILYMKGSPDAPQCGFSQRASQAVMACGK
                     RFAFVDVLSNPEIRANLPAYANWPTFPQLWVAGELVGGCDIITELHAKGELKALIDSA
                     VSE"
     gene            1032..2192
                     /locus_tag="PKZ52_12920"
     CDS             1032..2192
                     /locus_tag="PKZ52_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNG60719.1"
                     /translation="MTGMTLMNTYGNRSTTLVKGEGPYVWDSEGRRYLDGISGIAVCG
                     LGYNYPAVTQAIVEQAQKLLHTSNLYNIPSQQELGAKLVAISGMDKAFFSNSGAEANE
                     AAIKIARKYGNDKGIEQPHIITTQNSFHGRTLATLSASGNNKIQKGFYPLVEGFSHVP
                     YNDLAAIEAAATANTVAVMLEPVQGEGGIRVPSAEFLPKLRALCDAKGWLLILDEIQT
                     GNGRTGTYFAYQHYGFLPDVVTTAKGLGNGVPIGACLARGAAAEVFQAGNHGTTFGGN
                     PLACSTALAVVNSLDSAMLSRVTYLGERIKGSLQEALAGSPKVKEVRGQGLLIGIELS
                     EDCGALVEVGKQKGVLINVTAGNTVRLLPPFILTDEQADALVQAVVSLIQGF"
     gene            2202..3113
                     /gene="argF"
                     /locus_tag="PKZ52_12925"
     CDS             2202..3113
                     /gene="argF"
                     /locus_tag="PKZ52_12925"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNG60720.1"
                     /translation="MAARHFLTLNDISADELRFIIAQAIELKRRHRQGEIIETLKNRV
                     LGMIFEKSSTRTRISFEAGMTQLGGAALFLSPKDTQLGRGEPIEDSARVISRMVDIIM
                     IRTFGHDIIERFAAYSQVPVINALTDSFHPCQLLADIQTFVEHRGSPQGKKVAWIGDG
                     NNMCHSYINASRLCDFQLTIACPEGFEPDAALVARNADRVTLVRDPIEAAKGAHWIAT
                     DVWASMGQEAEQAERAAKFANYQVSHAHLKVAAADALFMHCLPAHRGEEVSAELFEDS
                     QYSVVWDEAENRLHAQKALLLFLLGKL"
     gene            complement(3172..3429)
                     /locus_tag="PKZ52_12930"
     CDS             complement(3172..3429)
                     /locus_tag="PKZ52_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60721.1"
                     /translation="MRLSADHLQAQGFTYYRAPQVAAAGRTFDAVATPGSAYEVRDST
                     SAPGAKASYSPPLTAPIRSGAGVFQAVAEFAPARPVIDIYV"
     regulatory      3570..3663
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3719..>4208
                     /locus_tag="PKZ52_12935"
     CDS             3719..>4208
                     /locus_tag="PKZ52_12935"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNG60722.1"
                     /translation="MPSSPPSRLVVTIAGSDCSGGAGIQADLKTFGVFGVFGASVITA
                     VTAQNSQEFYQANVLSPALFNSQWQAVVAGAPIAAIKLGMLGDGAMANTVADALEAFS
                     ADHPQVPIVIDPVLKASTGGDLSGEPSLKVYQERLIPLAHLLTPNIPEAAKLLNQTEA
                     INL"
BASE COUNT          947 a         1180 c         1164 g          917 t
ORIGIN      
        1 ctcgtgtaca cccacatcga atacggtcac tatgttgggg tgcaccagct gggagactat
       61 gcgggcttcg cggaaaaacc gcttgccgaa ggccgggtca tcggtgagcc cctgggccat
      121 cactttaagg gcaacctgcc gctcgaaaat gtcctgtacg gccagatata cagtcgccat
      181 accgcccttg ccaagggtgc ggatgatgcg gtaaccggga atatgcaggc tcatagcacc
      241 aacccgtgcg gtgcgctctt aggtgaaaag atgcattgaa gaaggcgggt ggtcataaaa
      301 aacaaaggct tggctacgag cgttcagtgt aaccaggcct tagcgggagg ggaacaaaaa
      361 aactctaggg cttattcaga tacagcacta tcgatcagcg cttttaactc gcccttggca
      421 tgcaactccg tgatgatgtc gcaaccaccg accaattcac cggccaccca gagttgtggg
      481 aaggtgggcc agttggcgta ggcgggtaaa ttagcgcgaa tctcagggtt ggagagcacg
      541 tccacaaacg cgaagcgctt gccacaggcc atcacggctt gggaggcgcg ctgggaaaaa
      601 ccgcactggg gggcatcggg ggagcctttc atataaagaa tgacggggtt ttgcgcgatt
      661 tgctctttga tggtttcgat aatgtccata gcggtaccta tgtggatgaa attaagaaag
      721 cgtggattaa attaagaaag cgataaccca gtgaaactct cgggtattgc ggcattttac
      781 tgtcaatggg gagcccggcg gtaccccttg tgcaggactt cttggtttta atcaacctct
      841 agccaataaa ggggttgtca aaggcaaaat attattttta gaatgccctt tgcaggcagc
      901 gatcgctgcc tgttttgttt ttgggctttt aaacctggaa acatcagttg agtctttcgc
      961 gggggcgtta atgcgcacaa gtacgaacta ggcggtgggt ttgactgagg cttctaggtt
     1021 ctctatccga gatcacaggt atgaccctca tgaacaccta cggcaaccgc agcaccactc
     1081 ttgttaaggg cgaaggcccc tatgtctggg atagcgaagg ccgccgttat ttggacggta
     1141 tctccggtat tgccgtttgc ggtttgggtt ataactaccc tgcggtcacc caggcgattg
     1201 tggagcaagc gcagaagctg ctgcacacct ccaacctcta caacatcccc agccagcagg
     1261 agctgggcgc taagttggtg gctatctccg gtatggataa agccttcttc tccaattccg
     1321 gcgctgaagc caacgaggcc gccatcaaaa ttgcccgcaa gtacggcaat gacaaaggca
     1381 tcgaacagcc ccatatcatc actacccaaa acagttttca tggccgcacc cttgcgaccc
     1441 tgtcagcgtc cggcaataac aagattcaaa aaggttttta ccccttggtc gagggcttca
     1501 gccatgtgcc ctacaacgac ctagccgcca ttgaagcggc tgccaccgct aatactgtgg
     1561 cggtgatgct cgaaccggta caaggcgaag gcggcatcag ggtgcccagc gccgagttcc
     1621 tgcctaaatt gcgcgccctc tgtgatgcaa aaggctggct gttgatcctg gatgaaatcc
     1681 aaaccggcaa cggccgcacc gggacctact tcgcctacca acactatggc tttttgcccg
     1741 atgtggttac taccgccaag ggcctgggca acggcgttcc tatcggcgca tgcctcgcta
     1801 ggggcgccgc tgccgaagta ttccaagcgg gcaaccacgg caccacgttt ggcggcaatc
     1861 ccctggcctg tagcaccgcc cttgcggtgg tcaacagttt ggatagcgcc atgctcagcc
     1921 gcgtcaccta tttaggcgag cgcatcaaag gctccctgca agaggcatta gcgggttcac
     1981 ccaaagtaaa agaagtgcgc ggccaaggcc tgcttatcgg catcgagctg agcgaagact
     2041 gcggtgccct ggtggaggtt ggcaagcaaa agggtgttct gatcaacgtc accgctggca
     2101 acaccgtgcg cttattgccg ccttttattc tcaccgacga acaagccgat gccctggtgc
     2161 aagcggttgt ctccctcatt caagggtttt aggaactcgc tatggccgct cgacactttt
     2221 taaccctcaa cgatatctcc gccgacgaac tgcgctttat tatcgcgcag gcgattgagc
     2281 tgaaaaggcg ccatcgccag ggcgaaatca tcgagacgtt aaaaaaccgc gtgctgggga
     2341 tgatttttga aaaatcctcc acccgcaccc gcatttcctt tgaggcgggc atgacccaac
     2401 tgggcggtgc cgcactattt ttatccccca aggacaccca gctcgggcgc ggcgagccca
     2461 tcgaggattc cgcccgggtc atttcccgca tggtggacat catcatgatt cgcaccttcg
     2521 gtcacgacat catcgagcgt tttgccgcct attctcaggt gccggtgatt aacgccctca
     2581 ccgactcctt ccacccctgc cagttattag cggatattca aaccttcgtt gaacaccgcg
     2641 gcagccccca gggtaaaaaa gtggcttgga ttggcgatgg caacaacatg tgccactcct
     2701 acattaatgc ctcgcgcctg tgtgacttcc agctcaccat cgcctgcccg gaaggctttg
     2761 agccggatgc cgcgctggtt gccaggaacg ccgaccgcgt aaccctggtg cgcgacccca
     2821 tagaagccgc taaaggcgcc cactggattg ccaccgacgt gtgggccagt atgggccaag
     2881 aggccgagca ggccgagcgc gcggctaagt tcgccaatta ccaggtaagc cacgcccatt
     2941 taaaagtggc tgcagctgat gccctcttta tgcactgcct gcccgcccac cgcggcgaag
     3001 aggtgagtgc tgaattattt gaggacagcc agtattccgt ggtctgggat gaagcggaaa
     3061 accgcctgca cgcgcaaaag gccctgctgc tgtttttgct gggtaagtta tagggttttt
     3121 agcagtgcac ttagcgacag ggacggggct ttttaagagg aaatattaat ttcaaacata
     3181 gatatcaatc accggccgag caggcgcaaa ctccgccacc gcttggaaaa cgccagcacc
     3241 tgagcgaata ggggcagtca gcggcggcga gtagctggcc ttggcgccgg gggcactggt
     3301 cgagtcacgc acttcatagg cactgccagg cgtcgccacg gcgtcaaagg ttctccccgc
     3361 agcggccact tgcggagcgc ggtagtaggt aaacccttgg gcctgtaaat gatcggcgga
     3421 cagcctcata agtgcacagc tcgtaaaaac ttcagtatag atttacatcg gggttgtgaa
     3481 agcggcctgg gctagtgggc aaaactcaca acttctcttg ggccaacatt tgtgtgcttt
     3541 acctagctgg gcttagcctc tagactggcc aggtcttgtc ggggtgctgt ttagatacgg
     3601 ctgagaaaaa cccgcaaacc tgatccggat aataccggcg tagggaacga ggcgcattat
     3661 tacaggtttt ctgcccgtct gttattgcgt cgcgctccga atttatttgg taaagccgat
     3721 gccctcttcc cctcccagcc gtttagtcgt caccatcgcg ggcagcgatt gctctggcgg
     3781 tgccggtatt caggcggatt taaaaacctt tggggtgttc ggcgtcttcg gcgccagtgt
     3841 gatcaccgcc gtcacggcgc aaaatagcca agagttttat caggccaatg tgctctcccc
     3901 tgcgctattt aattcccagt ggcaggctgt tgtagcgggc gcccctatcg cggccattaa
     3961 acttggcatg ttgggggatg gggccatggc caataccgtg gccgatgccc tagaagcctt
     4021 cagtgccgat cacccgcagg tgccgattgt cattgacccg gtacttaagg cctccaccgg
     4081 cggagatcta agcggcgagc ccagcctgaa ggtttatcaa gagcgcctga tccccctcgc
     4141 ccatctgctg acccccaata ttcccgaggc tgcaaaatta ctaaatcaaa ccgaagcgat
     4201 taacctag
//
LOCUS       DAOQGS010000365         4204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11429_length_4204_cov_4.006990, whole genome shotgun sequence.
ACCESSION   DAOQGS010000365 DAOQGS010000000
VERSION     DAOQGS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11429_length_4204_cov_4.006990"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..870)
                     /locus_tag="PKZ52_12940"
     CDS             complement(217..870)
                     /locus_tag="PKZ52_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNG60723.1"
                     /translation="MKLVSVFLLSLCLVACATNDPYTGERKTSNTTKGAGIGAAVGAL
                     LGAATSSKQDRRKGALRGAAVGGIIGAGTGHYMDKQEKVLRERLEGTGVRVQRNGKNI
                     KLIMPGNITFATGSAQIRQDFFEVLDSVVDVLKEFKDTRIRISGHTDSVGSDAMNQRL
                     SEERADSVKYFLLDQHVPAGRINAYGYGERYPVADNGTEAGRGQNRRVELELEPLEN"
     gene            1092..1553
                     /locus_tag="PKZ52_12945"
     CDS             1092..1553
                     /locus_tag="PKZ52_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60724.1"
                     /translation="MIRRPLKKHSFTPWIMSISLCLVLGACQNYPITFNGNRLNSAPL
                     LSGFNLADKHLSACVNEHIFDQKIRKAEQLTELNCAERSIHSLKGLEFFPHLLVLRLN
                     NNPIDSFKPVLALQNLERLEVGNTTADCASLMQLRQKGVKVEGACAGASAQ"
     gene            2333..2773
                     /gene="cynS"
                     /locus_tag="PKZ52_12950"
     CDS             2333..2773
                     /gene="cynS"
                     /locus_tag="PKZ52_12950"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNG60725.1"
                     /translation="MDKETMTRHILQAKLTKGASWEAMGKAIGVSEVWLASVCLGMNS
                     APKDKALAIAEYLDLGADISEALMAFPTKVWAQAVPTDPLIYRLYEIVGVYGETLKQV
                     IQEKFGDGIMSAIDFSMDVAKEENPKGDRVVLTLNGKFLPYKSW"
     gene            complement(2890..>4204)
                     /locus_tag="PKZ52_12955"
     CDS             complement(2890..>4204)
                     /locus_tag="PKZ52_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNG60726.1"
                     /translation="TTTTLYDAAYRSRLGAEKFMLDGRHTGTGGGNHVTLGGPTPADS
                     PLLRRPDVLQSLITFWQHHPSLSYLFSGMFIGPTSQAPRVDEGRDEMLYELEVAFAQI
                     PKGEVPQPWLVDRLLRNLLIDITGNTHRAEFCIDKLYAPGLSSGRQGILEFRGFEMPP
                     HERMALVQTLLLRCLFMRFWQKPYHKPLVRWGTELHDRFMLPHFIWSDMKEVVRDLNE
                     QGFAFDLAWLLPFEEFRFPHYGRVQVDNISLELRWAIEPWHVLGEEIGSFGTARYVDS
                     SVERVQVRLTGLTDSRYSLVCNGRHVPLQATGTHGEFVAGVRYRAWQPPSALHPTIGV
                     HAPLVFDVVDLWNSRSIGGCVYHVSHPGGRSYDTFPVNAFEAESRRGNRFLTVGHTQG
                     PFSPRPHLDGVRQFFPVVGNKPMAAPLVEVNPEYPHTLDLRRTTR"
BASE COUNT         1010 a         1130 c         1096 g          968 t
ORIGIN      
        1 cacccgcctt aaaagcccag acacaattgg atttaattat tgaagaagcc cccgccacac
       61 cgaccctggc aggtgcagta gatggatttg aggtattgat gatggcagga ttggcgctca
      121 agctgaggcg gcgcagggct gatcagaaag cttaaggggg ggcctattgc agcactctgg
      181 cgcggcaata ggctaaatgc tatttaattt tcctaactag ttttccaggg gctctaactc
      241 aagttcaacg cggcggtttt gaccgcgacc tgcctcggtg ccgttgtcag caactgggta
      301 acgctcgccg tagccgtagg cgttgatgcg gcctgcgggc acgtgttgat ccaataaaaa
      361 gtatttcacg ctatcggcgc gctcctcact taaacgctgg ttcattgcat cgctgccgac
      421 ggaatctgtg tggccggaga tgcggatgcg ggtatcttta aattctttta acacatccac
      481 tacggaatcg agcacttcaa aaaagtcctg gcgaatctgc gcgctaccgg tggcaaaggt
      541 gatattgccg ggcatgatga gcttgatgtt tttaccattg cgctgtacgc gcaccccggt
      601 gccctccaag cgctcacgca ataccttttc ctgcttatcc atgtagtggc cggtgccagc
      661 acctatgatg ccgccaaccg ccgcaccgcg cagggcgccc ttgcggcgat cctgtttact
      721 gctggtggcc gccccgagca gtgcacccac cgcggcgcca atacctgcgc ccttagtggt
      781 attactggtt ttgcgctcac cggtgtaggg atcattggtt gcacaggcaa ccaaacacag
      841 gctgagtaaa aacactgaca cgagtttcat agcggcattc tccgaaagtt agctgacgtt
      901 ttacggggct tgcgccattg tgcccggtat agccgcattt gcaacagcca aagcgcttca
      961 catgtgcaag cacaccatca aagtgtggac gatttagccc tatcagacag cgcttatatt
     1021 tactggcgcc gggttctaat gcccaggaca ggccaggtac aattcccacc tgcccattca
     1081 cgagtcatct tatgatccgc cgccccttaa aaaagcattc ctttacccct tggattatga
     1141 gtattagcct gtgcttggtg ctcggcgcct gccaaaatta cccaatcacc tttaacggca
     1201 atcggttaaa tagcgcgccc ttattaagcg ggtttaatct tgcggacaaa cacttaagcg
     1261 cctgcgttaa cgagcatatt tttgatcaaa agatcaggaa agccgaacaa ctgactgaac
     1321 ttaactgcgc cgagcgctct attcactcat taaaagggct ggagtttttt ccgcacctac
     1381 tggtgctgcg gcttaacaat aatcctatcg actcttttaa accggtcttg gccctgcaga
     1441 atttagagcg ccttgaagtg ggcaacacca cagccgactg cgcaagcctt atgcaattgc
     1501 gccagaaagg tgtgaaggtt gagggcgcct gcgcgggcgc atcggctcag taggctttaa
     1561 taattacacg gcattttgcg attgttttcc tggcgtttgt tcctggttaa atcccaattt
     1621 ttaggcatct tatccaagtt ctttagtgag tttccttgcg ggtttaacaa agagtaaggc
     1681 acaacattac tcttttggca gagtaatggg atttagcaat taaaggcaaa caaccgtaaa
     1741 aaattcgtac ctattcagcg ccattttgtt ggagctggat gtgattcccg tgccgaacag
     1801 aaatcaccag gcgaatgttt tggcgctgtt agccctcagc actcccccgc agtgataaat
     1861 acaggttatt tcagacacgc cgtgaataca tccgtctagg ctcgccaaca aggttcctcg
     1921 ttttgacgct ttgaaataac ctgtattcat ccctgctgca ccgcgtatcg attctgccac
     1981 ttggctaaat ccgccttaat ttgccgcccg cgcttaagag ggcaataagg cgtactgaat
     2041 agttaaaaat tcattgataa aaaactgtct tttcgctctg cgctaaccga ttagcatcac
     2101 cgcctaaatt taccctcctt gcctgagaaa ttagccatta tttatttcct gttacttggg
     2161 tttaagctaa aaaatgcagt caaacctaat gcagtcttta ggcataacgg tttctcagta
     2221 gcgcaaaaat aatgcgcact cgtccatggc attaatcctg caaaattatc cactcactcg
     2281 gggcttaggc tcctattatt aatcgacctg aggaaattgc atgttagccg ccatagacaa
     2341 agagaccatg acccgccaca tactgcaggc taaactgacc aagggcgcca gctgggaagc
     2401 aatgggcaaa gctatagggg tatcggaggt gtggttggcg tcggtgtgcc tggggatgaa
     2461 ttcagccccc aaggataaag ccctcgccat tgcggagtat ttggatttgg gcgccgatat
     2521 tagcgaggcg ctcatggcct ttcccaccaa ggtatgggcg caagcggtgc cgactgaccc
     2581 gcttatttac cgcctctatg aaattgtcgg cgtctatggg gagacgctca aacaggttat
     2641 tcaggaaaaa tttggcgatg gcatcatgag cgccatcgac ttttctatgg acgtagccaa
     2701 agaggaaaat cccaaaggcg acagagttgt gttgacctta aacggtaaat tcttacccta
     2761 taaaagctgg taaaactagg cttttaagcc tgtttttact tactcagcgc agccacaaat
     2821 accgccaaca aaaaagcccg ccgaatcaac agcgggcttt tttttaccgc attccctgcg
     2881 gtttttggct tagcgggtgg tgcggcgcaa atccaaggtg tggggatatt ctgggttaac
     2941 ctccaccaaa ggcgccgcca tgggtttgtt gcccaccaca gggaaaaact gccgcacacc
     3001 atccaaatgg ggcctgggcg aaaatggccc ctgggtgtgg ccaacggtta agaagcggtt
     3061 accccggcgg gattccgcct cgaaggcatt gaccgggaag gtatcgtagc tgcggccgcc
     3121 gggatggcta acgtggtaca cgcagccacc gatggagcgg ctattccaca gatccaccac
     3181 atcaaatacc aagggtgcat gcacgcctat ggtggggtgc agggccgagg gcggctgcca
     3241 ggctctatac cgcacccccg ccacaaattc accgtgggtg ccggtggctt gcaagggtac
     3301 atggcggccg ttacacacca gtgagtagcg gctgtcggta aggccggtca ggcgcacctg
     3361 cacccgctcc accgaggaat ccacatagcg ggccgtgccg aagctgccga tttcttcgcc
     3421 caacacgtgc cagggctcga tggcccagcg cagttccagg ctgatgttgt ccacctgcac
     3481 gcggccataa tggggaaagc ggaattcttc aaagggcagc agccaagcta gatcaaaggc
     3541 aaaaccctgc tcgtttaaat cccgcaccac ctctttcata tccgaccaaa taaaatgcgg
     3601 cagcataaag cgatcgtgca gctcggtgcc ccagcgcacc aagggcttgt gatagggctt
     3661 ttgccagaag cgcataaaca ggcagcgcaa taacagcgtt tgcactaggg ccatgcgctc
     3721 gtgagggggc atctcaaagc cgcgaaactc caaaataccc tgccggccag aacttaagcc
     3781 cggggcatag agtttgtcga tacaaaactc cgcccggtga gtattgccgg tgatgtcgat
     3841 taacagattg cgcaatagac gatccaccaa ccagggctgt ggcacctcac ctttggggat
     3901 ttgcgcaaag gccacctcca gctcatagag catttcatcg cgaccttcat caaccctcgg
     3961 cgcctgggag gtggggccga taaacatccc cgagaagaga taagacaagc tgggatggtg
     4021 ctgccaaaag gtgattaggc tttgcagcac atcggggcgc cgcagcaggg ggctatccgc
     4081 aggggtcggc ccgccgaggg tgacgtggtt gccgccgcca gtgccagtgt ggcggccatc
     4141 caacatgaat ttctcggccc ccaaacggct gcggtaggct gcgtcataaa gggttgtggt
     4201 ggta
//
LOCUS       DAOQGS010000366         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11517_length_4183_cov_4.781250, whole genome shotgun sequence.
ACCESSION   DAOQGS010000366 DAOQGS010000000
VERSION     DAOQGS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11517_length_4183_cov_4.781250"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1082..3400)
                     /locus_tag="PKZ52_12960"
     CDS             complement(1082..3400)
                     /locus_tag="PKZ52_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNG60727.1"
                     /translation="MSPTETLRNFTVGAIEVSPQHNTLRLVNRSITVQPKVMAVLYYL
                     AQHQTRVVGNEELLQAVWQGRVVTLASVQKSINALRSALADLAGEQEFVAYFSKRGYQ
                     LMQPVSWVEVSSRSQNESAPLVPAAPPEPPSNAAEADVTSSSNTHPNSSPTTVALPLN
                     EPPPKISAPKTAALPLKIIMGMVLIGVIILAAFYWLYTPKSAAPNTATQRPAPVAPAP
                     TDTAETAGKKLPWQIITHYLPAEANAHHTTPSPDSKRAAYLRDTEIAGGAHQSDLMIR
                     DTTGTDWLLARSNSAWVDLAWSPSGRALAALEIYRADGLLKDPDFYQSPQYLYNIHLF
                     TLDLKGQHLLEKNLLSQWQGKVNSITWWDENTLEFVATMGANLTKERYRYSITDQTLT
                     SLAPLPTGFQPLISRVRDKTTALISRLQDKTQVEFLDEQQNLLGKQLIAASIKDLSWA
                     ADGGGVYGLETQQNSIYFLPIQGSGYSAPLPAPLHNMPPGGLGNLKINGDGKSIFVST
                     SSQDVQLYQHNPQGLSLLADVHNLIHSSVVYSPAGDGVIYATHQNNQAQIWLLKNGNT
                     EALYKTDGPIQTILWPGADYLLVKANNKIARYQLDKHSWSVLLEDAQDLTPLAYQADT
                     QALIAVKNINDVRNIWRINLETHQYKQLTFGEVGTARHWNERILFQYANQAGLWQLNT
                     QEPNHKAMGDQLPQHSQLLGATGEQVYFVTGGPCHESDIQQFDLIHNTVTTYLKRRDN
                     NVRSMDFSAQQGSLQIPCTTNSYISYEIRAAD"
     gene            3683..>4183
                     /locus_tag="PKZ52_12965"
     CDS             3683..>4183
                     /locus_tag="PKZ52_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60728.1"
                     /translation="MNRLAVVNFLVCMVWASWAAAAPNKVYLNENVGFNVKGYKYTQK
                     TFPCEVDKKLIVSLLDQGKKQSIPMELAGTPEKLRNGTIPVLAVDIEQLALGKEGFNF
                     SRSKNNSLPMIQATAAIITGGNVVSSKHTCAFATLNEFTPAGSVLDLGTATTVCRAMR
                     KCVGDLG"
BASE COUNT          975 a          894 c         1139 g         1175 t
ORIGIN      
        1 gttgccccta gaagggttaa ataatatttg gctttaatca tatagtgtgg ctcgcgcagt
       61 ggaatcgatt tgaagtgcag tgtggttgaa actatggatt tacctatgcc tgtgggctga
      121 acctgatcaa tagtgttatt tcgcgcgcag cttgagtgca tccgctgttg gcgtgctaag
      181 cggtttttct tttgcttggc cttaaataac aggtaattgg gtctgttggc tggggaatga
      241 acgtgcaagt ccaatctcaa ttcttagcaa tgtcgagttg agattggggg tataacagaa
      301 ggcataatgg tttgttgaag tgtgcaacta atgcataaaa cctccgtggc tagcgtgcga
      361 gtaatctttt tgtattttgc gcgtgatcta tcgtttaatc gaaaatgatt aaaatcctgt
      421 ccgccaggta tgggtgtgta tgtgtactat ctgcgtagca gttgcgccgc tgctgatgct
      481 atttttagca atgctttaga gagctgtata tgtgtgcgtg ctgatagtca cagaaaggtt
      541 ttaagtgggt aatgctgagg tagtgggtag gccaaaaggg agatttaggt gctggcggcg
      601 ccagcgccaa tacagccagg ccgatttgta tttaacaagc tgttaatggg ttgtttgaat
      661 gtagctattc agcagctttt tattggtatt ggacgttatt ctccagcaaa ttagaaaata
      721 ccaggcgagt tattgggggc tgcgaatccg cagtgatgaa tacaggttat tgcagacacg
      781 ccgtgaatgc atccgtgtag gctcgtcaaa aaggtccctc ttgttaacgg tctgaaataa
      841 cctgtattca tcactgctgc accgcgcatc gattaggcgg gttggctaaa tccttctcaa
      901 tttgccaccc gagcggagag agcaggaatg cctgctgaat agttgcgttt gaattgcgtt
      961 caggagccac agattaaata tggggtggcg actattttta ttaacctggc actaattgcc
     1021 accgggttaa tagaaacact atgagcagaa gtacttcagc ggatatagac tgtaaacagt
     1081 attaatccgc tgcacgaatc tcatagctta tataggagtt ggtggtgcac ggaatctgta
     1141 ggctgccctg ttgagctgaa aaatccatgc tgcgcacatt attatctcgg cgtttcaggt
     1201 aggttgttac tgtattgtgg attaaatcga actgctggat atcagattca tggcaggggc
     1261 cgcccgtgac aaaatagact tgctcgccgg tagcacctaa taattggctg tgttggggca
     1321 gctggtcgcc cattgcttta tgattaggct cttgtgtgtt taattgccac aaacccgctt
     1381 ggttggcgta ttgaaacaat atgcgctcgt tccagtggcg cgctgtgcca acttcgccaa
     1441 aggtcagttg tttgtattgg tgggtctcta aattaatgcg ccaaatattg cgtacgtcat
     1501 taatgttctt aacggcaata agtgcctgcg tgtcggcctg ataggccagc ggtgttaaat
     1561 cctgtgcgtc ctctagcaat acactccagc tgtgtttatc cagctgatac cgagcgattt
     1621 tattgttagc ttttaccagg agataatccg cccctggcca cagaatagtc tgaatcggtc
     1681 catcggtttt atacagcgcc tcggtgttac cattttttaa tagccaaatc tgcgcctgat
     1741 tattttgatg ggttgcgtaa ataacgccat caccggcagg tgagtagact acgctgctgt
     1801 gaattaaatt atgcacatcc gccaggaggc tgaggccttg aggattgtgt tgatacaact
     1861 gtacgtcttg gctgctggta cttacaaaaa tcgatttgcc gtcgccgttg atttttaaat
     1921 tgccaagccc gccgggcggc atattatgca gtggtgcggg caggggggcg gaatatccac
     1981 tgccttgaat gggtaaaaaa taaattgaat tttgctgggt ttctaggcca taaacaccgc
     2041 cgccatccgc cgcccagctg agatctttaa tactggccgc tattaattgt ttgcctaata
     2101 ggttttgttg ttcatctaaa aattccactt gggttttatc ctgcaagcga ctaatcagtg
     2161 ccgtggtttt atcgcgcacg cggctaatca gtggttgaaa ccccgtcggc aatggcgcaa
     2221 ggctggtgag ggtttggtcg gtaatgctgt agcgatagcg ctctttagta agattggccc
     2281 ccatagtggc gacgaattca agggtatttt catcccacca ggtaatagaa ttcactttgc
     2341 cttgccattg gcttaacagg tttttttcca gcaggtgttg gccctttaaa tccaaagtaa
     2401 ataaatgaat attgtataaa tactgcggcg attgataaaa atccgggtct tttaataaac
     2461 cgtcggcgcg gtagatctcc agtgctgcca gagcgcgccc actgggggac caggctaaat
     2521 ctacccaggc actattgctg cgcgctaaca gccaatccgt accggttgta tcgcgaatca
     2581 ttaaatcgct ttggtgggca ccacctgcta tttccgtatc gcgcaaatag gcggcacgtt
     2641 tgctgtctgg gcttggcgtg gtgtgatggg cgttggcttc ggcgggtaaa taatgggtaa
     2701 taatttgcca gggtaacttc ttgccggctg tttcggcagt gtctgtagga gccggtgcaa
     2761 ctggtgcagg ccgctgtgtt gccgtgtttg gggcggctga tttaggtgtg tacagccaat
     2821 aaaaagcagc cagaataatc acgccgatca acaccatccc catgattatt tttagtggca
     2881 gggcggcagt tttgggcgct gaaattttcg gtggtggctc gtttaacggc agtgctaccg
     2941 ttgtagggct tgaattggga tgggtatttg aactgctggt tacatcggcc tcggcggcgt
     3001 tgctgggggg ctcaggtggc gccgcaggga caagtggcgc ggattcattt tggctgcgcg
     3061 agcttacttc gacccagctg accggttgca tcagttgata gccgcgtttg gaaaaatacg
     3121 cgacaaattc ctgctcgcct gctaaatccg ccagggcgct gcgcagggcg ttaatacttt
     3181 tttgcaccga ggccagtgta accacccggc cttgccacac cgcttgcagc aactcttcat
     3241 tgcccaccac ccgcgtttgg tgttgggcga ggtaatacaa caccgccatc accttgggtt
     3301 gaacggtaat ggagcggtta accaggcgca gcgtattgtg ttgtggagat acttcaattg
     3361 cgcctaccgt aaaattgcgg agcgtttcgg ttgggctcac cctgggtgct tcctattatg
     3421 tagttataag gtaaagatgc agcttggcgt gcgacagccg tgttcaacta tcgatggcaa
     3481 cagcggaatt gtgccggatc aggtaggcta aagacaagca aaattgtttc ccttaaaatg
     3541 cataactatt tgatttgcaa tggtatattt ttggtaagcg aagggtgtgg cacaggtata
     3601 ggtgcggccc gacaattttg gttgtgctta ggtatagttt attcatcccc gcaggccggg
     3661 gctatggttt acggggggcg ttatgaatcg cttagcagta gtaaattttt tagtgtgtat
     3721 ggtatgggct tcttgggcag ccgcagcgcc caataaggtc tatttgaatg aaaatgttgg
     3781 ctttaatgtg aaggggtata aatacacgca aaaaaccttc ccttgtgagg tggataaaaa
     3841 gctaattgtc agcctgcttg atcaaggcaa aaaacagagc atacccatgg agctggcggg
     3901 cacccctgaa aagttgcgca atggcaccat cccggtgctt gcggttgata tagagcagct
     3961 ggccttaggc aaagaaggtt ttaattttag tcgcagtaaa aataacagcc tgccgatgat
     4021 tcaggccact gcggcgatta tcactggcgg caatgtggtc agctccaaac acacctgcgc
     4081 ctttgccacc ctcaacgaat ttactccagc gggcagcgta ttggatttgg gaacggccac
     4141 aaccgtgtgc cgcgccatgc gcaaatgtgt gggcgatttg ggc
//
LOCUS       DAOQGS010000367         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11518_length_4183_cov_4.762597, whole genome shotgun sequence.
ACCESSION   DAOQGS010000367 DAOQGS010000000
VERSION     DAOQGS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11518_length_4183_cov_4.762597"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1903)
                     /locus_tag="PKZ52_12970"
     CDS             complement(<1..1903)
                     /locus_tag="PKZ52_12970"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNG60729.1"
                     /translation="MKLFRSISLALALILLVSGVVLISTFDAKRFLPVITQAAQKEGF
                     ALHVDDIGLAIWPELGLKLTGIQVDSLARPGPTPLLTMAKATLAIPIKPLLAGTLQVR
                     EISLDNGAVNLWQNKTGNNWSGAPAGDMQQNKSAATQTPDTGSNKVATNKIEINKVAL
                     KNIALQWQDDQQPPMAASIKQAGVELKELNHFAVTLVGQLAARGTTPALAVELEQTLK
                     QADASWQLLDGKGKIGPVNGPQFLINYHGDLSDNFAKVAGGFSVQPFNPAAYLPLLGI
                     TLADKSRLQSLSISADVNWHAGALKFTQGILKLDDTAINWQLMQNAERWQLAIKAPRL
                     DVDAYLPPPNPTEEKAQHTSEPPPEIDLSGVPAMDISAQFDELIIARYVLNELHFKLA
                     VDSRRIALAPVSARLYGGALDGQLNISLAPKSAELKGNLDAKGVNLEPLWKHFSPQAQ
                     MQLSGAINAQTQWQATATGLSGLAKQLNGQVTVQGEGVRLSPLNITQHYCQIISQATL
                     WKKDNNEWPEFSQLQELTGEFAITPAQVTFNRLYSRVDKLQVDGAGSWDRQSDQLRVQ
                     LPITLLQMAEAETNIKGCTIGSDYWVNRKLSLLECKGKLATLDAKRDCGIDKAGVAGL
                     LKDYALYKLK"
     gene            2295..2795
                     /locus_tag="PKZ52_12975"
     CDS             2295..2795
                     /locus_tag="PKZ52_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60730.1"
                     /translation="MLEIITHTPTWVFGLFIGLVLLGVQQRKNRQVKKPLAFILPLGM
                     LALSFTGVWTSFGFMPSALSIWGAGLIMSAFAVARIWPVRGLKYNQTNNSFFIPGSWA
                     PFSVIMLIFFAKYSVGVLHGISPAALDSTLLKLAFSLVYGLSSGYFVGRALCLWQAFS
                     QKRAGI"
     gene            complement(2864..3916)
                     /locus_tag="PKZ52_12980"
     CDS             complement(2864..3916)
                     /locus_tag="PKZ52_12980"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNG60731.1"
                     /translation="MSIFKSHITAMSAYKPPLDGRDAHQHLLLDFNERTLPVAKAVED
                     ALVDYIRSGRLQMYPSYGNVVSQIAAYAGVPAEQLMITNGSDQGIELIIRASCREGDE
                     AIIPGPGFAFYAQCAKVENLSIIEPKYRFNEGFPFNQVMAAITPKTRVICLANPNNPC
                     GTPISREHILAIAEAAPQAAILVDECYYEYVKTTVADVVEQYPNIVITRTFSKTWGIP
                     SLRLGYIISHPNNILALLNVRGPYDVNQLAVVAIQAALQNPKATLAYVDEVMQQSKPL
                     LEEYLRNKAVKFWPSATNYIWTFPADPQAVTAGLQQAGILVRPRLDADGVLGVRITIG
                     TLEQTQRLIATLDGLL"
     gene            complement(4046..4171)
                     /locus_tag="PKZ52_12985"
     CDS             complement(4046..4171)
                     /locus_tag="PKZ52_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60732.1"
                     /translation="MPNVACVGGSWLVPKAAVDAQNWQAITDIAKQTTAKLTPKS"
BASE COUNT         1043 a         1084 c          948 g         1108 t
ORIGIN      
        1 tttttaattt atacagcgca tagtctttta ataggcctgc cacgcccgct ttatcaatcc
       61 cacaatcgcg cttagcatct agggtggcca atttgccttt acactctaag agggataatt
      121 tgcgattcac ccaataatca ctgccaatgg tgcagccttt gatattggtt tcagcttccg
      181 ccatctgcag taaagtaatc ggcaattgca cccttaattg atccgactgc cgatcccaac
      241 tgcctgcccc atccacctgt aatttatcca cccggctgta taagcgatta aatgttacct
      301 gcgctggggt aatggcaaat tcgccggtta attcctgcaa ttggctaaac tccggccatt
      361 cgttattatc ttttttccac aaagtggcct ggctaattat ttggcagtag tgctgggtaa
      421 tattcaatgg gcttaagcgt acaccctccc cctgcaccgt cacctggcca tttagctgtt
      481 ttgccaaacc actaaggcct gtagccgttg cttgccattg ggtctgcgcg ttaatggcgc
      541 cgcttaattg catttgagcc tgggggctaa agtgcttcca tagtggctcc aggttcacgc
      601 ccttagcatc aagatttcct tttagctcgg cggatttggg tgcaaggcta atattcaact
      661 gcccatctaa agcgccgcca tatagccgag cagaaacagg cgccaaggct atgcggcgag
      721 aatcaaccgc caatttaaag tgcaactcat ttaatacgta acgagcaatg atcagctcat
      781 caaactgcgc gctgatgtcc atggcaggca caccgcttaa atctatctca ggtggaggct
      841 cagaagtgtg ttgcgctttt tcctcagtcg gattgggcgg cggtaaataa gcatccacat
      901 ccagtctggg agccttaata gccaattgcc aacgctcggc gttttgcatt aattgccaat
      961 taatggcggt gtcatctagt tttaaaattc cctgggtaaa tttaagcgcg cccgcgtgcc
     1021 agtttacatc cgcgcttata cttaaggatt gcagacggct tttatccgcc agagtaatac
     1081 ccaaaagcgg caaataagct gcaggattaa atggctgcac actaaaacca ccggccactt
     1141 tggcaaagtt atcgcttagg tcgccgtgat aattaattaa aaattgcggc ccattgactg
     1201 ggcctatttt tcccttgcca tcgagcaact gccagctggc atcggcttgt ttaagtgtct
     1261 gctcaagctc aacggccaaa gccggtgttg taccgcgcgc cgctaattgc cctactaaag
     1321 tgacggcaaa gtgatttaat tctttaagct caacgcccgc ttgtttaatg cttgcggcca
     1381 ttggcggctg ctgatcgtct tgccattgca gggcaatatt cttaagcgct actttattaa
     1441 tttctatttt gttagtcgct actttattac tccctgtatc cggtgtttgt gttgccgctg
     1501 atttattttg ttgcatatca ccggcaggcg ccccgctcca attgttcccc gttttatttt
     1561 gccaaagatt gaccgcgcca ttgtcgaggg aaatttcccg cacctgcagt gtgccagcta
     1621 acaaaggctt aatgggtatg gcaagtgtcg ccttagccat tgttaataaa ggcgtaggcc
     1681 caggccgcgc cagtgaatcc acctgtatcc ccgtaagctt aaggcccagc tccggccaaa
     1741 tggctaaacc tatgtcatcc acgtgaaggg caaaaccctc tttttgcgcc gcctgggtaa
     1801 ttacgggtaa aaaccgcttg gcgtcgaagg tgctaatcag cacaacgcca cttaccagta
     1861 agatcagcgc taacgccaga gaaatgcttc gaaataattt cacactaatg ctcctatgct
     1921 accaatgggc ttaatttgcc cgaacttatt aacccggggg caaaatagat cgtcctgatc
     1981 tattttgccg tcgctgcgtt aaacttacga aaatgcgtgg attttcttgt tttaacgcag
     2041 ctcgcggcgg cttgcaccgc cgagtgcggc atccttgccg ccattattaa cccggcatta
     2101 aatgccgccg ggttaataac actatacgca aactggctaa tgaggcgagc ctaatgatgc
     2161 atggcccagc aagctgcccc cccaataggc ttacttgtat ccgtaaagtg gcattatcga
     2221 atggattaaa ataatcccaa acagtaccat gagccacgct caatttgacc ccacattttt
     2281 cttaaggaaa cgctatgctc gagattatta cccacacccc cacctgggta tttggactat
     2341 ttattggttt ggtgctactt ggggtccaac agcgcaaaaa tcgacaagta aaaaaacctt
     2401 tggcgtttat tctgcctttg ggcatgttgg cgctttcgtt caccggtgtc tggaccagct
     2461 ttggctttat gccaagcgcc ctatcgatat ggggtgccgg cttaattatg agtgcttttg
     2521 ctgtagcgcg aatctggcct gtgcgcggct tgaaatataa ccaaacaaac aacagctttt
     2581 ttatccccgg cagttgggca ccgttctcgg taattatgct gatatttttt gccaaatatt
     2641 ctgtgggggt tttacacggc ataagtcccg ccgctttaga ctctacccta ttaaaactag
     2701 catttagctt agtatacggt ttatccagcg gttatttcgt cggccgcgcg ctctgcctat
     2761 ggcaggcatt tagccaaaaa agggctggca tttagccaaa aaaatacccc gtaaaacgag
     2821 acagctttac ggggcgatta attgcctaaa gcaggttaat cacctaaagc aaaccatcca
     2881 aagtggcaat taagcgctgg gtttgctcaa gagttccaat ggtaatgcgc acacctaata
     2941 caccatcggc atccaaacgc ggtcgcacca aaatgcccgc ctgctgtaaa cctgcggtaa
     3001 ccgcttgcgg gtcggcggga aaggtccaaa tataattagt ggcactgggc caaaatttta
     3061 ccgccttgtt gcgcagatac tcttccaata agggtttaga ttgctgcatc acttcatcca
     3121 cataggccag agtagcctta gggttttgta atgccgcttg aatggccacc accgctaatt
     3181 gattgacatc gtaggggccg cgcacattta acagcgccaa aatattgttg gggtggctga
     3241 tgatataacc caagcgcaaa ctgggaatgc cccaggtttt tgaaaacgtg cgcgtaataa
     3301 caatattcgg gtattgctcc accacatcgg caacagtggt ttttacatat tcgtaataac
     3361 actcatccac caaaatagcc gcctgaggcg ccgcctccgc aatcgctaaa atatgctccc
     3421 ggctaatcgg cgtgccgcag gggttattgg ggtttgccag acaaataacg cgagttttag
     3481 gagtaatcgc cgccatcacc tgattaaatg ggaagccctc attaaagcga tatttcggct
     3541 caataatgct tagattttcc accttcgcgc actgggcata aaacgcaaaa ccagggcctg
     3601 gaataatcgc ctcatcacct tcgcggcaac tggcgcgaat aattaattca ataccttggt
     3661 ctgagccatt ggtaatcatc aactgctcag ctggcacacc ggcataggcg gcaatttgtg
     3721 acactacatt gccgtaggac ggatacattt gcaggcgacc agagcgaata tagtccacca
     3781 aagcatcttc caccgccttg gcaaccggca aagtgcgctc attaaaatcc aacaacaaat
     3841 gctgatgcgc atcccgccca tccaatgggg gtttataggc gctcatcgca gtgatgtggg
     3901 atttaaagat agacatatta gcctcctgtg aattggacag gcattatacg ccgagggctt
     3961 ggtaaatagc cgcccaggtt aaaccgcttc cataaaaaaa ccgcctaaag gcggttttaa
     4021 ttctgcggct tacatcctct tggccctagc ttttaggtgt gagcttcgcc gtggtttgtt
     4081 tggcgatatc ggtaatagct tgccaatttt gcgcatccac cgccgccttt ggcaccaacc
     4141 aagagccgcc cacacaagcc acattgggca gggctaaata ctg
//
LOCUS       DAOQGS010000368         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11664_length_4147_cov_4.010264, whole genome shotgun sequence.
ACCESSION   DAOQGS010000368 DAOQGS010000000
VERSION     DAOQGS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11664_length_4147_cov_4.010264"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /gene="prfB"
                     /locus_tag="PKZ52_12990"
     CDS             <1..829
                     /gene="prfB"
                     /locus_tag="PKZ52_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153894439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNG60733.1"
                     /translation="NDEGSLGDIQKEIDQLEAQLAKLEFRRMFSKEMDPCNAYLDIQA
                     GSGGTEAQDWADMMLRMFLRWGEAKGFKTTLEECSDGDVAGIKSATIRFEGEYAFGWL
                     RTETGVHRLVRKSPFDSGNRRHTSFASVFVSPEISDDIQIDINPADLKVDTYRASGAG
                     GQHVNKTDSAIRITHVPTGVVVSCQSERSQHSNRDKAMKMLRAKMYEQEMLKRNAEKQ
                     ALEDSKADIGWGSQIRSYVLDDSRIKDLRTGVQTSNCQAVLDGDLDDFIEASLKAGL"
     gene            845..2353
                     /gene="lysS"
                     /locus_tag="PKZ52_12995"
     CDS             845..2353
                     /gene="lysS"
                     /locus_tag="PKZ52_12995"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNG60734.1"
                     /translation="MTDQSQDENKLIAERRSKLAHIRSQGNAFPNQFRRSHEAQALQN
                     SHGEYSKEELEAMDFKVAVSGRILAKRGPFFVLQDASGRIQLYADKDAQHVMKERWHN
                     WDIGDIVGVTGALHKSGKGDLYVNCEEYQLLTKALRPLPDKYHGLADQELRYRQRYVD
                     LVVNPEIRDVFKLRTKIIDFIRSYLNGRDFMEVETPMLQVIPGGATAKPFITHHNALD
                     IQMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGLSTRHNPEFTMLEFYQAYADYND
                     LMNLTEDMLRQLSSAVLGTNLVTATKPAKEGEEPVQVVYDFAKPFTRLSVFNSILHYN
                     PELTADNINTLEAAKKVAEGLKIPVKPSWGLGKVQIEIFEKTVEHRLEQPTFITEYPA
                     EVSPLARRNNDNPFVTDRFEFFVGGREIANGFSELNDAEDQAERFQSQVAEKDAGDDE
                     AMHFDSDYIRALEYGLPPTAGEGIGIDRLVMLLTDSPSIRDVLLFPHMRPEA"
     gene            2360..3037
                     /gene="ung"
                     /locus_tag="PKZ52_13000"
     CDS             2360..3037
                     /gene="ung"
                     /locus_tag="PKZ52_13000"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNG60735.1"
                     /translation="MGKNIELEPSWLAHLQAEFNAPYMQQLRAFLQQEKQQGQVVYPP
                     GPLMFNALNTTPFDAVKVVILGQDPYHGPGQAHGLSFSVPDGVPPPPSLVNMFKEINT
                     DIGLPISKNGNLTKWARQGVLLLNATLSVRAGLAGSHQGRGWEQFTDKIIDLLNKQRE
                     DLVFLLWGSYAQQKGKLIDTRRHLVLKAPHPSPLSSHRGFFGCKHFSQANHWLQSKGL
                     PIIDWAV"
     gene            3037..4101
                     /gene="thiO"
                     /locus_tag="PKZ52_13005"
     CDS             3037..4101
                     /gene="thiO"
                     /locus_tag="PKZ52_13005"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNG60736.1"
                     /translation="MARVTGQRIAIAGAGLMGRLLAWRLALLGHSVHLFDTDSIKSPK
                     NAAHTAAAMISPLSEVVVSERLIYDMGMQSLAIWPKWIAELNHQQHPIISYSDQGSLV
                     IAHPQDEAELQQFYQDLIHHLGAEHGARWLNRAELKQLEPDLASNFARGLLLPFEAHL
                     DNRQLLDNLLAQLNQLGVQLHEHTSINLDTPDQYGQFNLWLDCRGTGAKDGPTPQIVR
                     GVRGEVLWVHTEEVHLNRPIRLMHPRYKLYIVPKPGGRFIIGATELESEDRSPMSLQS
                     LLELGSALYTLNPAFAEARILELDANLRPSLMDNLPHIDNGQLNNGCPFVRVNGLYRH
                     GYLLAPCLVGRVLEEIVWLA"
BASE COUNT         1111 a         1047 c         1029 g          960 t
ORIGIN      
        1 aaacgacgag ggcagcctgg gcgatattca aaaggaaatt gatcagctag aagcccaatt
       61 ggcgaaactg gaatttcgcc gtatgttcag taaagaaatg gacccctgta atgcgtacct
      121 ggatatccag gcgggctccg gcggcaccga ggcgcaggat tgggccgata tgatgctgcg
      181 gatgtttttg cgctggggtg aggccaaggg atttaaaacc actttagaag aatgctccga
      241 cggcgacgta gccgggatta aaagcgccac cattcgtttt gaaggtgaat acgccttcgg
      301 ctggctgcgc acggaaaccg gtgtacaccg cttagtgcgc aaatcgccgt ttgattcggg
      361 caaccgccgc catacctcgt ttgcgtcggt gtttgtgtcg ccggaaatct ccgatgatat
      421 tcaaattgat atcaacccgg cggatttaaa agtggacacc taccgcgcca gtggcgccgg
      481 tggccagcac gtcaacaaaa ctgactcggc catacgcatc acccacgtac ccaccggcgt
      541 ggtggtaagt tgccagagcg agcgctcgca acattccaac cgcgataaag ccatgaagat
      601 gctgcgcgca aaaatgtacg agcaggaaat gcttaagcgc aacgcagaaa aacaagccct
      661 ggaagacagc aaagcagata taggctgggg cagccaaatt cgctcctatg tgctggatga
      721 ttcgcgcatt aaagatttgc gcaccggggt acaaacttct aactgccagg ccgtattaga
      781 tggcgacctg gatgatttta ttgaagctag cttaaaagct ggcctgtaaa aaacgagcca
      841 aaccatgact gatcaaagcc aagatgaaaa caaattaatt gccgagcgcc gcagtaaact
      901 tgcgcatatc cgcagccagg gcaacgcctt cccgaaccaa tttcgccgca gccacgaagc
      961 gcaagcattg caaaactccc acggcgaata cagcaaagaa gagttagagg cgatggattt
     1021 taaagtcgcc gtcagcgggc gtattctcgc taaacgcggg ccgttttttg tattgcaaga
     1081 cgcctccggc cgcatccaac tctatgcaga taaagacgcg caacatgtaa tgaaagagcg
     1141 ttggcataac tgggatattg gcgatatcgt cggcgtaacc ggcgcactgc ataaatccgg
     1201 taaaggggat ttatatgtga actgcgagga ataccaatta ctcaccaaag ccctgcgccc
     1261 actgccggat aaataccatg gcctcgccga ccaagaattg cgctaccgcc agcgctatgt
     1321 ggatttagtg gtaaacccag aaattcgcga tgtatttaaa ctgcgcacaa aaatcatcga
     1381 ttttatccgc agttatttaa acggccggga ttttatggag gtggaaaccc ccatgctgca
     1441 ggtaattccc ggcggcgcca ccgccaaacc atttattacc caccacaatg cgctggatat
     1501 tcaaatgtac ctgcgcatcg ccccagaact atatttaaag cgtttagtgg tgggcggttt
     1561 tgagcgggtg tatgaaatta accgtaactt ccgcaacgaa ggcttaagca cccggcacaa
     1621 cccagaattt accatgctgg agttttacca agcctatgcc gattacaacg atttaatgaa
     1681 tttaaccgaa gacatgctgc gccaattaag cagcgcggta ttgggtacta atttagttac
     1741 tgccaccaag cccgccaaag aaggcgaaga gccagtgcaa gtggtatacg attttgccaa
     1801 accctttacc cgcctgtcgg tatttaattc tatattgcac tacaacccag aattaaccgc
     1861 cgacaatatc aataccttgg aagccgccaa aaaagtcgcc gagggcttaa aaatccctgt
     1921 aaaacccagc tggggtttag gcaaggtaca aattgaaatc tttgaaaaaa ctgtcgagca
     1981 ccgtttagag cagcccacct ttattaccga atacccagcg gaagtctcgc ccttagcgcg
     2041 gcgcaataac gacaatccat ttgtgactga ccgctttgaa ttttttgtcg gcgggcggga
     2101 aattgccaac ggtttttcgg aattaaacga tgcggaagac caggccgagc gtttccaaag
     2161 ccaagtagcg gaaaaagacg cgggcgatga cgaagccatg cattttgact ccgattatat
     2221 tcgcgcgctg gaatacggcc tgccccccac tgctggcgaa ggtataggca tcgaccgttt
     2281 agtaatgtta ttaaccgact ccccttctat tcgcgatgtt ttattgttcc cccatatgcg
     2341 gccggaagcc taaagtatta tgggtaaaaa catagaactg gagcccagct ggctcgcgca
     2401 tttacaggcg gaatttaatg cgccttatat gcaacagctg cgcgcctttt tgcagcagga
     2461 aaaacaacag ggtcaggtgg tttacccacc cggccctttg atgtttaacg ccctgaacac
     2521 cacgccgttt gacgcggtaa aagtagtcat tctaggccaa gacccctacc atggccccgg
     2581 ccaagcccat ggtttatctt tctccgtgcc cgatggcgtg ccgcccccgc catccctcgt
     2641 caatatgttt aaagaaatta atacggatat tggcctgccg atcagtaaaa acggcaattt
     2701 aaccaaatgg gcacgacagg gggtgttatt actcaatgcc actttaagcg tgcgcgcagg
     2761 ccttgccggt tcccaccagg ggcgcggttg ggagcaattt accgataaga ttatcgacct
     2821 gctgaataaa cagcgcgaag atttagtatt tttattgtgg ggttcatacg cccagcaaaa
     2881 aggtaaatta attgatacgc gcagacacct agtattaaaa gccccccacc cttcgcccct
     2941 ctccagccat cgcggttttt ttggctgtaa acatttctcc caggcaaacc actggttgca
     3001 gagcaaaggt ttgccaataa ttgattgggc cgtttaatgg ccagggttac agggcaaaga
     3061 attgccatcg ccggtgctgg tttaatgggc cgtttattgg cctggcgttt ggcgctactc
     3121 ggccacagcg ttcacctgtt tgatacagac tccataaaaa gccctaaaaa tgccgcccac
     3181 accgccgccg ctatgattag cccgctgtct gaagtggtgg taagcgagcg gcttatttac
     3241 gatatgggta tgcagtcctt ggctatttgg cctaagtgga ttgcagaatt aaatcatcag
     3301 caacatccca ttattagcta tagcgaccaa ggcagcttag tgatcgccca cccgcaggat
     3361 gaagcggaat tacagcagtt ttatcaggat ttaattcacc atttaggcgc tgaacacggc
     3421 gcccgttggt taaaccgcgc agaactaaaa caattagagc cagaccttgc cagtaatttc
     3481 gcgcgcggtt tattgctgcc ttttgaagcc catttggata accgccaatt attggataat
     3541 ttattggcgc aattaaacca attaggtgtg cagctacacg aacacaccag tattaattta
     3601 gatacacccg accaatacgg ccaatttaat ttatggctgg actgccgagg caccggcgcc
     3661 aaagacggcc ccaccccaca aatagtgcgc ggcgtgcgcg gtgaagtact ttgggtacac
     3721 accgaagaag tgcacttaaa ccgccctatt cgtttaatgc acccgcgcta caaactctac
     3781 atagtgccca aacccggcgg gcgttttatt atcggcgcca ccgaattaga aagcgaagac
     3841 cgctcaccta tgagcctgca atccctgttg gaattaggca gcgccttata taccttaaac
     3901 ccggcctttg ccgaggcgcg gattttagaa ttagatgcga atttgcggcc tagtttgatg
     3961 gataatttgc cccatattga taatgggcaa ttaaataatg gttgcccgtt tgtgcgggtg
     4021 aacgggttgt atcggcatgg atatttgttg gcgccttgtt tggtggggcg ggtgctagaa
     4081 gagattgtgt ggttagcttg aggtgctatt aagtagttgg gtagagcata gcgaaaccca
     4141 acacagt
//
LOCUS       DAOQGS010000369         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11666_length_4147_cov_3.457722, whole genome shotgun sequence.
ACCESSION   DAOQGS010000369 DAOQGS010000000
VERSION     DAOQGS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11666_length_4147_cov_3.457722"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="PKZ52_13010"
     CDS             <1..1562
                     /locus_tag="PKZ52_13010"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HNG60737.1"
                     /translation="SFNALLKTLEEPPEHVKFLLATTDPQKLPVTVLSRCLQFNLKNM
                     SPERIVGHLGFVLGEEQIPFEEAALWQLARAADGSMRDSLSLTDQAIAFGCGKVASSD
                     VAAMLGTIDYQLIAQVMQGLIDGDGRALLQAVAAFAEHAPDYFGALGDLVTLLHRVAI
                     AQALPEAVDNSFGDRQQILHFSRALTPEDVQLFYQTALIGRRDLHLSPDPRAGFEMTL
                     LRMLAFKPQGLLDLPQAPLHFAGGQGAASSAPAGLPLAEPVAPPDLPPQPEPPSKTSF
                     VATAPADEAPPQMHDARADPLAFAPPAPEPLYDSTGIDNAQAAPVEPLVDASGEAGPD
                     AKKPQPLSAELSAQLGLTRAANLIHERKSRDKPAAVEAQVAAPAVVSVPAAGIRLEDF
                     TPAHWVSVYQALNLRGVIQSTASNAVLTGREGNQLYFLLDEARASLYEDSHRQRLADA
                     LSDYFGQPIKAHINIGALAPEHLTPAQAGINERAEKLQQGIATLRADPFVQQLVRDFD
                     GELKIDSVKVL"
     gene            1576..1893
                     /locus_tag="PKZ52_13015"
     CDS             1576..1893
                     /locus_tag="PKZ52_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNG60738.1"
                     /translation="MGLGDLMKQAQAMQEKMQSMQEEMANREVEGQAGAGLVKVVMTG
                     RHDVRSVSIDPSVMSEDKEVLEDLLAAAVNDAVRRIEELNKSSMGNLAAGLNLPAGFK
                     FPF"
     gene            2275..2868
                     /gene="recR"
                     /locus_tag="PKZ52_13020"
     CDS             2275..2868
                     /gene="recR"
                     /locus_tag="PKZ52_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNG60739.1"
                     /translation="MLSPLIDQLIASLRTLPGVGPKSAQRMALHLLERNRPAALQLAA
                     NLQAACEQVKRCDSCRILTEQTRCHICASPKRQAEVLCVVENPADVMALEQSGIFRGR
                     YFVLYGKLSPIDGIGPAELGLDVLFERLRSDGISELIIATNPTMEGEATAHFIAQKAK
                     PLDVKVTRIAHGVPIGGELEYIDGGTLAHALNSRREL"
     gene            2871..4046
                     /gene="rnd"
                     /locus_tag="PKZ52_13025"
     CDS             2871..4046
                     /gene="rnd"
                     /locus_tag="PKZ52_13025"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNG60740.1"
                     /translation="MSAATASPQTPTPIWVASDSALAQLCTAWNQQAWLAVDTEFLRT
                     NTYYPIAALLQIYDGHAIYFIDPTAISQWQPLAQVMANPAVCKALHACSEDLEIFALL
                     LGALPVNLFDTQVAAAMVGQGFSVGYARLVEQMLGVHLPKDETRSDWLARPLSPAQTL
                     YAAWDVYYLLPIAQQLHAKLTEQNRLAWLQEDCNSLLAHSQDAQDAALSHLRLKGLQR
                     MSRREQALAARLSFWREQVAQETNVPRNHVVKEAALYQLAQSKPKHISQLRHIEDLPE
                     KTIRRYGEDIIEMIQSVLALKDEQLPPPQPRPLSGSQQQLIKNWRLWVEAQAQTLAVA
                     PEVLARKRDYEYLARGLPKGEPLPSILQGWRRSIIGDGLLALCTREPDTALAQFSAE"
BASE COUNT          892 a         1216 c         1187 g          852 t
ORIGIN      
        1 gcagttttaa cgcgctgctt aaaaccctcg aagaaccccc cgaacacgtc aaatttttac
       61 tcgccaccac cgacccgcaa aaactgccgg tgacggtgct ctcccgctgt ttgcaattta
      121 acctgaaaaa catgagcccc gagcggattg tggggcactt ggggtttgtt ttgggggagg
      181 agcaaatccc ctttgaagag gcggccctgt ggcagctggc ccgcgccgcc gacggcagta
      241 tgcgcgacag cctgagcctt accgaccagg ccatcgcctt tggttgcggc aaggtggcca
      301 gcagcgatgt ggcggctatg ctcggcacca tcgattacca attaatcgcc caggtgatgc
      361 agggcttgat tgatggcgac ggccgagcgc tcttgcaggc tgtagctgcc tttgccgagc
      421 acgcaccgga ttactttggc gccctcggtg atttggtgac cctcttacac cgagtggcca
      481 ttgcccaagc tttgcccgag gcggtggata atagctttgg cgatcgccaa cagattttgc
      541 attttagccg cgccctcacc ccggaggatg tgcagctctt ctaccaaacc gccttgatcg
      601 gccgccggga tttgcattta agcccagacc cgcgagccgg ttttgaaatg accctgctgc
      661 gcatgctggc cttcaaaccc cagggtttgc tggatttgcc ccaggcgccc ctgcattttg
      721 caggcgggca gggggctgct tcgagcgcac cagccggtct ccctttggca gaaccagtgg
      781 cgcccccgga cttgccgccg cagccagagc ccccgtccaa gaccagtttt gtggctacag
      841 cgcccgcaga tgaagcaccg ccccagatgc atgatgcccg cgcagaccct ttagcctttg
      901 cgccgccagc acctgagccc ctgtacgatt caacaggtat tgataacgcg caagcggccc
      961 ctgttgagcc cttggttgac gccagtgggg aggctggccc tgatgcaaaa aagccccagc
     1021 ccctcagcgc tgagctatct gctcagctgg gccttacgcg cgcggcgaat ttaattcacg
     1081 agcgcaaaag ccgcgataaa cctgccgccg tagaagccca ggtggctgca cccgcggtgg
     1141 tgagtgtgcc tgcggcgggc attcgcctgg aagacttcac acccgcccat tgggtgagtg
     1201 tgtaccaggc gctcaatctg cgcggggtaa ttcaaagcac ggcgtccaac gcggtgttaa
     1261 ccggccggga gggcaatcag ctgtattttt tattggatga ggcccgcgcc agcctgtacg
     1321 aggattccca ccgccagcgc ttggccgatg ccctaagtga ttattttggc caacccatca
     1381 aggcacatat caacataggt gctctggccc cggagcatct aacccccgcc caagcgggca
     1441 ttaatgagcg ggcggaaaaa ttacagcagg gcatagccac cctgcgcgcg gacccgtttg
     1501 tgcagcaact ggtgcgcgac tttgacggcg aattaaaaat tgacagcgta aaagtactct
     1561 agaggagcaa atgctatggg cctgggtgat ttaatgaaac aagcgcaagc catgcaggaa
     1621 aaaatgcaat ccatgcagga ggaaatggcc aaccgcgaag tggaaggcca ggccggtgcc
     1681 ggtttggtaa aagtggtgat gaccggccgc cacgacgtgc gctcggtaag tatcgacccg
     1741 tctgtaatga gtgaagataa agaagtattg gaagacctct tggccgccgc cgtcaatgac
     1801 gcagtgcgcc gcattgaaga gctgaataaa tccagcatgg gcaatttagc ggcgggatta
     1861 aatttgccgg ccgggtttaa gttcccattt taatcaaccg gcgccaatca gggactgcca
     1921 ggctgaacgc cccatttgca cagctgattg atgcggcgct gttgtcggcc caatgataga
     1981 gcggtgcttt gcactcgcgc caactcaccg atgtgggagt gtgcgcccat cgtgaattgt
     2041 gtttgggcgc cggggcgcag gatgtggtgc cctaaatact cgcccagctt ggttcgggca
     2101 gatcaagccg gagggaattt agccctggga attttaagtt ttgaagttac tttaagtttt
     2161 gaagttactt taagttttga agttacagcg cccggcggcc aaaccagagc accagataca
     2221 acgcacgcag caagacactt ttattaacgc tttacccaga gtaagccagg cccgatgtta
     2281 agccccctaa tcgaccaact aatcgcctcc ctgcgcaccc tgcccggtgt gggccctaaa
     2341 tccgctcagc ggatggcgct gcatttattg gagcgcaacc ggccagcggc cctgcagcta
     2401 gccgccaatt tgcaggcggc ctgcgaacag gtcaaacgct gcgatagctg ccggattctc
     2461 accgagcaga cccgctgcca tatttgcgcc agccccaagc gccaagcgga ggttttatgc
     2521 gtcgttgaaa acccggctga tgtgatggcc ctggagcagt cggggatttt tcgcggccgc
     2581 tattttgtgc tctacggcaa gctctccccc atcgatggca tcgggcctgc ggaattggga
     2641 ttggatgtgt tattcgagcg cctgcgcagc gatggcatca gcgaattaat tattgccacc
     2701 aaccccacca tggaggggga ggccacggcc cactttatcg cccagaaggc caagccctta
     2761 gatgttaaag tgacccgcat cgcccacggt gtgcccatcg gcggcgagct tgaatacatc
     2821 gatggcggca ccctggccca tgcccttaac agccgccggg agctttaatc atgtccgccg
     2881 ccacagcttc cccgcaaacc cccaccccta tctgggtggc aagtgatagt gcgcttgcgc
     2941 agctgtgcac cgcttggaac caacaggcct ggttggcggt ggatacagaa tttttgcgca
     3001 ccaacaccta ttaccccata gccgcgctgc tgcaaatata cgatggccac gccatttatt
     3061 ttatcgaccc caccgccatc agccagtggc agcccttggc gcaggtgatg gccaaccccg
     3121 ctgtgtgcaa ggccttacat gcctgttcag aggatttaga aattttcgcc ttgctgctgg
     3181 gcgctttgcc ggtgaatctt tttgataccc aggtggcggc ggccatggtg gggcaggggt
     3241 tttcggtggg ctatgcccgt ttggtggagc agatgctggg tgtgcacttg cccaaagacg
     3301 aaacccgctc cgactggctc gcccgcccct taagccccgc gcaaaccctc tacgccgctt
     3361 gggatgtgta ttacctgctg cccattgccc agcagttgca cgccaaattg accgagcaaa
     3421 accgcctcgc ctggctgcaa gaggattgta attccctcct cgcccacagc caggacgccc
     3481 aggatgcggc gttaagccat ctgcgcttaa aaggtttgca gcgcatgagt cggcgcgaac
     3541 aggcccttgc ggcgcgcctg agtttttggc gggagcaggt ggcccaggag accaatgtgc
     3601 cgcgcaatca cgtcgttaaa gaggcggccc tgtatcagtt ggcgcagagt aagcccaaac
     3661 acatcagcca gttgcgccac attgaagacc tgccggaaaa aaccattcgg cgctacgggg
     3721 aggacattat tgaaatgatc caatcggtat tagcgcttaa agacgagcaa ctgccgccgc
     3781 cccagccgcg ccccttaagt ggcagccagc aacagctgat taaaaactgg cgcctgtggg
     3841 tggaggctca agcccaaaca ttggcggtgg cgccggaagt gttagcccgc aagcgggatt
     3901 acgaatattt agcgcggggc ttgcccaagg gcgagccact gccgagcatt ttgcagggct
     3961 ggcgccgctc gattatcggc gatgggctgt tggccctctg tacacgcgag cccgacacag
     4021 ctttagccca attttccgcg gaataaaaag cctatgaccg atgcaaccgc tccagcaaaa
     4081 ttaatttgcg atgtgtataa aagcgttaaa aaacaggatg tgtatttata tgtcagtaag
     4141 gccgagg
//
LOCUS       DAOQGS010000370         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11724_length_4135_cov_5.087010, whole genome shotgun sequence.
ACCESSION   DAOQGS010000370 DAOQGS010000000
VERSION     DAOQGS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11724_length_4135_cov_5.087010"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1666)
                     /locus_tag="PKZ52_13030"
     CDS             complement(119..1666)
                     /locus_tag="PKZ52_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNG60741.1"
                     /translation="MDIRRLLSDSLSLAFAHQPDAIAVTFGEQDYTYRDLYQQSQSIA
                     AYLREQGLKPGERVAIFMENSWPIVPSIFGVLYAGGVFLVINHLTKAHKLAYILQDSG
                     AQFLLSDAVVAGQFLPVLNQCPEVRAVIYAGATIAADCHKPLVPLASVLTTQVDTPVN
                     PGRINLDLAALIYTSGSTGDPKGVMMTHANMVFTCDSLIEYLRLDQQARILCFLPLAF
                     DYGLYQILMAVRLGARLIVCKSFAFPAQVFNEINRTQASVFPGVPTVFSTLIGIHHKT
                     PLCFPSVTRVTNTAAALPPEYVPILKTVFPNALIYKMYGLTECKRVSYLEPEDAERFP
                     TSVGKAIPGTQVFILDEARKPLGPNQMGTLYVRGAHVMQGYWNKPIETAKMLVKDLFP
                     GENILCAQDQFMLDEEGNMYFCGRVDDIIKSRGEKVSPVEVENVLYRLPGVREAAVLG
                     IDDPVEGQSIKAFVAIDADSGLDEKKIRQFCTAHLENFMVPKVVEIRESLPKTDSNKI
                     SKKDLRS"
     gene            complement(1677..1928)
                     /locus_tag="PKZ52_13035"
     CDS             complement(1677..1928)
                     /locus_tag="PKZ52_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNG60742.1"
                     /translation="MTDNTPRESLRQFILNNYLFTDDQSALTDDASFLDNGILDSMGI
                     LELIDFLDETFGVKVAGDELVPENLDSINSVLKFLATKQ"
     gene            complement(1931..3862)
                     /gene="asnB"
                     /locus_tag="PKZ52_13040"
     CDS             complement(1931..3862)
                     /gene="asnB"
                     /locus_tag="PKZ52_13040"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNG60743.1"
                     /translation="MCGILGLVAANPAPPEVVKGQLAAMAARLVHRGPDAEGFYCQGP
                     VGLGHRRLSIIDLEGGAQPMQTPDGQLCVTFNGEIFNFIELRQSLQQKGYVFASHSDT
                     EVILHLYREYGLGFANYLNGQFAIGLWDATKQQLILVRDRVGICPLYYFSDEQQFIFA
                     SEIKALMPALPQGLELNHEGLDDLFTGWTCLPPQTIFRAIWQVQPGEMLVIKNPSSIS
                     RHFYWQLNYPQYSSEYDPRSEAELIDELHQLLTDATAIRLRSDVPIGAYLSGGLDSSV
                     LVALMHRQIKDQLHTFSLGFTDPALDESVFQDRVVNYLGTQHARRTIDYPQIADALVA
                     TIWHTESPILRTAPVPMGLLSQWVQQQGYKVVLTGEGADEVLGGYDIFKEAKVRQFWA
                     RQPASTWRPHLLTRLYPYMELPKGDASVYLKRFFGVGLETPELLWHSHMPRWQTTSKA
                     KLFFGENFSAGLQHSFEAKINALFPPQASRWHSFNRAQFLEIKTLMSGYLLTSQGDRM
                     LTQHSIEGRFPFLDHRLMEFANRLPPKYKMKAMREKYLLRAMAKRYLPADISLRHKQP
                     YRAPNLAASQDALFNSDLADYLSAETCQRWGIFAGNKVGFLLQKARGAKALNTSEAQA
                     LTGILTTHILLDQFCSGRN"
BASE COUNT         1105 a         1016 c         1052 g          962 t
ORIGIN      
        1 gctgctgggg tttcctcact tttatcgggc aatacagcca attgaggggc aaactgctct
       61 agcaactgag taaactgccc tgcacattcc tgagcataga gttcggcaaa tagcggcatc
      121 atgagcgcaa atccttttta ctaattttat tggaatcggt tttgggtaaa ctctcgcgaa
      181 tttccactac cttgggcacc ataaaatttt ccaaatgggc ggtgcaaaat tggcggattt
      241 ttttctcgtc caggccgcta tcggcatcga tggccacaaa ggcttttatg gattgcccct
      301 ccaccgggtc atcaataccc agcaccgccg cctcccgcac gccgggcaaa cggtacaaaa
      361 cattttccac ctccaccggg cttacctttt cgccacggga tttaataatg tcatccaccc
      421 ggccgcaaaa atacatattg ccctcttcat ccagcataaa ttgatcctgg gcacagagaa
      481 tgttttcacc ggggaacaga tcttttacca gcattttggc ggtttcaatc ggtttgttcc
      541 agtagccttg cattacatgg gcgccgcgca catatagggt gcccatttgg ttggggccta
      601 agggtttgcg cgcttcatct aaaataaata cttgggtgcc aggaatggct ttacccacag
      661 aagtaggaaa gcgctcggca tcttcaggct ctaggtaact cacccgcttg cattcggtaa
      721 ggccgtacat tttataaatg agtgcattgg ggaaaacagt ttttaaaatc ggcacatatt
      781 ccggcggcaa agcagcggcg gtattagtca cgcgggtaac cgacggaaaa cacaggggcg
      841 ttttatggtg aatgccaatc aaagtggaaa acaccgttgg cactccggga aatacactgg
      901 cctgggtgcg gtttatttcg ttgaatacct gggcgggaaa ggcaaaggat ttacacacaa
      961 ttaaacgcgc acccaaacgc accgccatta aaatttggta caggccataa tcaaaggcga
     1021 gcggtaaaaa acacaggatg cgcgcctgct gatccagcct taaatactca attaaactat
     1081 cgcaggtgaa caccatattg gcgtgggtca tcatcacgcc tttagggtca ccggtgctgc
     1141 cggaggtata aattaacgcc gccaaatcta aattaatgcg gcctggattc accggtgtat
     1201 ccacttgagt agttaacaca ctggccagcg gcaccaaggg tttgtggcaa tcggcggcta
     1261 tggtggcacc ggcataaatc acagcgcgca cctccgggca ttgatttaac accggcaaaa
     1321 attgccccgc cacaacagcg tcggataata aaaattgcgc gccgctatcc tgcaaaatat
     1381 aagcaagttt atgggctttg gtgaggtggt taatcaccaa aaatacgccg ccagcgtaga
     1441 gcacaccaaa aatactcggc actatgggcc agctattttc cataaatatg gccacccgct
     1501 cacccggctt taagccctgc tcgcgcaagt acgcagcgat gctttggctc tgctggtaca
     1561 ggtcgcgata ggtgtaatcc tgctcgccaa aggtaaccgc aatggcatcg ggctggtgtg
     1621 caaaggcgag gcttaggcta tcggataata ggcggcgaat atccataaaa aaacccttat
     1681 tgcttagtgg ccagaaactt cagcacgctg ttaatggagt cgagattttc cggcactaat
     1741 tcatcccccg ccacttttac gccgaaggtt tcatctaaaa aatcgatcaa ctctaaaata
     1801 cccatggagt ctaaaatgcc gttatctaaa aatgaggcgt catcggtaag ggccgattgg
     1861 tcgtcggtaa acaaatagtt attgagaata aattggcgca aagactcgcg gggggtgtta
     1921 tcggtcatgg ttaattcctg ccactacaaa attgatcaag aagaatatgg gtggtgagta
     1981 taccggtgag ggcctgggct tcactggtgt ttaaggcttt ggcgccacgg gctttctgca
     2041 gcaaaaagcc caccttattg cccgcaaata tgccccaacg ctggcacgtt tcggcactta
     2101 aataatcggc caaatcgcta ttaaataaag catcttggct ggcagcaagg ttgggtgcgc
     2161 ggtagggctg tttatggcgc aaactaatat ctgcgggcaa atagcgttta gccatggcgc
     2221 gcagcaaata tttttcccgc atggccttca ttttatattt gggcggcaag cggttggcaa
     2281 attccattaa acggtgatct aaaaacggaa aacgcccttc aatggagtgt tgtgtcaaca
     2341 tgcgatcacc ctgggaggtg agcagataac cggacataag ggttttaatt tctaaaaatt
     2401 gtgcacggtt aaaactgtgc cagcggctcg cttgcggcgg aaataaggcg ttgattttgg
     2461 cttcaaaaga atgctgaagg cctgcggaaa aattctcgcc gaaaaataat ttggctttgc
     2521 tggtggtttg ccagcggggc atatgggagt gccagagtaa ttcgggggtt tccaggccca
     2581 cgccaaaaaa ccgctttaaa tagacgctgg catcgccctt gggcaattcc atataggggt
     2641 ataagcgggt taataaatgg ggccgccagg ttgaggccgg ttgccgagcc caaaattgcc
     2701 gcaccttggc ttctttaaaa atatcgtaac cacctaatac ttcatcagcg ccctcaccgg
     2761 ttagaaccac tttataaccc tgctgctgca cccattggga taataaaccc ataggtactg
     2821 gtgcggtgcg taaaataggg gattcggtat gccaaatggt ggcgacaaga gcgtcggcaa
     2881 tttgcgggta gtcgatggtg cggcgggcat gttgggtgcc taaataattc accacccgat
     2941 cttgaaaaac actttcatcc aaggccggat cggtaaaacc taaagaaaag gtatgcagct
     3001 gatccttaat ttgccggtgc attaaggcca ccagcacgga ggaatctaaa ccgccggata
     3061 aataggcgcc tatgggtaca tccgagcgca ggcggatagc cgtggcgtcg gttaataact
     3121 ggtgcagctc atcaatcagc tcagcttctg atcgcgggtc gtattcactg ctgtactgcg
     3181 ggtaattgag ttgccaataa aaatgccggc tgatgctgga gggattttta atcaccagca
     3241 tttcaccggg ttgcacctgc caaatagcgc ggaaaattgt ttgcggcggc aggcaggtcc
     3301 agccggtaaa taaatcgtct aatccttcgt gattcagttc aagcccctgg ggcagcgcag
     3361 gcattaaggc ttttatttcc gaggcaaaaa taaactgctg ctcatcgcta aaataataca
     3421 gcgggcaaat acccacccga tcacgcacga gaattaattg ctgcttagta gcatcccaca
     3481 ggcctatggc aaattggcca tttaaataat tcgcaaaccc caggccgtat tcgcggtaca
     3541 gatgcagaat aacttcggtg tcggaatgac tggcaaatac ataacccttc tgttgaaggg
     3601 attggcgcag ctcaatgaaa ttaaaaattt ccccattaaa ggtgacgcac aactggccgt
     3661 ccggggtttg cataggttgg gcgccgcctt ccaggtcgat aatactcagg cggcgatggc
     3721 ctaggcccac cggcccctgg caataaaagc cctccgcatc aggccctcgg tgcaccaggc
     3781 gcgctgccat ggccgcaagc tgccccttca ccacctcagg cggcgcaggg ttggccgcaa
     3841 ctaggccgag gattccgcac atatggatct ccgttaacag gggcttatgg gttttattgt
     3901 tagaaagggc gtaaaggcgg caaaattcgg gccgataaag cccaaagcat agcagccaac
     3961 agggcttata gcacttgata agggaaggcg ttaactgagt ttagatataa cgcaaataaa
     4021 accgctggat gagagccata aacacggcgc caggcaacgg gcaaacagca ggggggcttg
     4081 aacctaaaaa ccgcagttga actctactac ggccgcctaa tacttgaatc tgcac
//
LOCUS       DAOQGS010000371         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11749_length_4131_cov_3.724485, whole genome shotgun sequence.
ACCESSION   DAOQGS010000371 DAOQGS010000000
VERSION     DAOQGS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11749_length_4131_cov_3.724485"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKZ52_13045"
     CDS             <1..989
                     /locus_tag="PKZ52_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60744.1"
                     /translation="STPSPQDGMASTLRPDFLKAKFASASIFNYVIKYRISSSAVIVT
                     SIKLNKDILGAQKDSSKERSALYEIERIGKVKFTSSSSLSEIESLKDDSWRAKGAVRK
                     VVTRHAAVNGMLNDYRKAGWLMGVHADVAFGKDEFDSFTLFHNPSQTAKLDFYESVRD
                     NLGVTTENAKQLSAILRQVQQDGQPVKWVVHSQGGIIFKQALAYHLKHYSGQSLNLNS
                     VVFHAGGNNKRVTDKLLNKVGIRREAPDRDNPFDMVPNLAGFNHLSPAAITRSLRFSK
                     KVAGENSSIAESPHTLPFLTLEAYHHFLVLAGDFKSAKYVDRYMQKQARKGH"
     gene            1047..1793
                     /locus_tag="PKZ52_13050"
     CDS             1047..1793
                     /locus_tag="PKZ52_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60745.1"
                     /translation="MFSIVSQLFGCVRAAALSGPDFNQSIEKVIHVGHRVSVFYLIPG
                     NLSRELNFDKIYQRDTAKSYQINPEKLTSQKTLTWREFESIDMGTWDYWGKKSQGPGG
                     QLGSLRVSIGYSLLPKGADLIEHVKQSYQEYLNGAKGLNQKYRVDDMGKPVSPEEYAE
                     DAIAPPSKFSNTTVGGAQFITWETDREFDGFKNSPYIYYVLPIDQHGYLTFLFRPTIS
                     VFGQDMIAKQEQRIQQDINTFLSHIRVKPI"
     gene            complement(1911..3530)
                     /gene="nifA"
                     /locus_tag="PKZ52_13055"
     CDS             complement(1911..3530)
                     /gene="nifA"
                     /locus_tag="PKZ52_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HNG60746.1"
                     /translation="MRPSSFTKPASRIGSRSELLERQFSALCKIAHKLSWEKNIEDTL
                     TGILSVLHNDARLLHGLITLADPMHTVLHVGAVYSEDENLSKSTASVRYRAGEGVLGR
                     ILQHNQPIVLARISSEPRFLDRLSLYDLELPFIAIPIRDGDGAAIGVLAAQPDVAADD
                     YLSERTTFMEAVANLLSQTVRLLVNMEKGRTIASERDELRREVRAKYGFDNMVVGRSE
                     GMRQVFDQVRRVAKWDSTVLILGESGTGKELIANAIHYNSPRAHNQFVRLNCASLPET
                     LLESELFGHEKGAFTNAVKQRKGRFEQADGGTLFLDEIGEISPMFQAKLLRVLQEGEL
                     ERVGGGQTIKVDVRIVTATNRNLEVEVDKGRFREDLFYRLNVMAIRLPPLRERIVDIP
                     ELSQFLLSKIAKHQGRELRLTDSALRLLMQHDWPGNVRELENSLERAAVMSEEGTIDS
                     DVITLASSFYTPSSPHPSQHNAPSYNNHITPPSLNSADLLNPAVDERERLIMALEQAG
                     WVQAKAARLLGMTPRQIAYRIQIMKINVRKM"
     gene            complement(3543..>4131)
                     /locus_tag="PKZ52_13060"
     CDS             complement(3543..>4131)
                     /locus_tag="PKZ52_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60747.1"
                     /translation="VANRDDFVLNALEEAINAGQQALDNLNAALPPPLQEAKKPVNIN
                     QLIRDAIALSTVSLLSQGITIEWKPSLYLPAVIGREQRLLGMFKQLIDNAIEAMSDRK
                     IDYRELSIITHGEKDRVICEITDTGSGIKPELIVKVFEPFYSTKPPGRSCRGMGLSMV
                     QEIVADHAGTVFIDPSYREGCRVVVHLPLTSAGRS"
BASE COUNT         1100 a          993 c          970 g         1068 t
ORIGIN      
        1 caagcacacc ctcacctcag gatggtatgg cgagcacttt acgaccagat tttttaaagg
       61 cgaagtttgc cagtgccagc atttttaact acgtaattaa atacagaatt tcttcgtcgg
      121 cggttatcgt tacttcgata aagttaaata aagatattct cggtgctcaa aaagactcgt
      181 ctaaggagcg ctcagcacta tatgagattg aacgtattgg caaggttaaa tttaccagta
      241 gctcctcact cagtgaaatt gaaagtctta aagatgatag ctggcgcgcg aaaggggcgg
      301 taagaaaagt agtcactcgc cacgcggcgg ttaatggcat gctcaatgat taccgaaagg
      361 caggctggct gatgggagta catgcagatg tcgcttttgg taaggatgaa ttcgactcat
      421 tcaccctttt ccacaaccca agtcaaaccg caaaattaga tttttatgaa tcagtgcgag
      481 acaacctagg tgtcacaaca gaaaatgcca agcaattatc agctattttg cgtcaggttc
      541 aacaggacgg ccagccagtg aaatgggtag tacacagcca aggtgggatt atctttaaac
      601 aagctcttgc gtaccatctc aaacactact ccggacaaag ccttaacctc aattcagtgg
      661 tctttcatgc cggtggcaac aataagcgag taactgataa attgttaaat aaagtaggta
      721 ttcgccgcga agcacccgat cgcgacaatc cctttgatat ggtgcctaat ctcgcaggtt
      781 ttaatcactt aagtccggcg gcaatcaccc gcagcttgcg ttttagtaaa aaagtagctg
      841 gagaaaacag cagcatcgcc gaaagtcccc acaccttgcc ttttttgaca cttgaggcct
      901 atcatcactt tcttgtactc gcaggtgatt ttaaatcggc taagtatgta gaccggtaca
      961 tgcaaaagca agcgcgcaag gggcactagt caccccaact cagacacatg cataccatga
     1021 attgcattca caaaaagatt ctcattatgt tttctatagt tagccaactt ttcggttgcg
     1081 tacgagcggc tgcactttca gggccagact ttaaccaatc cattgagaag gttatccatg
     1141 ttggccatcg ggtaagtgtt ttttatttga tacccggcaa cctctcccgc gaattaaatt
     1201 ttgataaaat ttatcagcgc gataccgcta aaagttatca aatcaatcct gagaagctga
     1261 ctagccaaaa aaccttaacc tggcgcgagt ttgaatctat cgacatggga acttgggatt
     1321 attggggtaa aaaatctcag gggccaggtg ggcagttggg tagtttaaga gtcagcattg
     1381 gatacagctt attgcccaag ggtgctgatc tcattgaaca cgtcaagcag tcttatcaag
     1441 aatatttaaa tggcgcgaag ggactcaatc aaaaatatcg agttgatgat atgggcaaac
     1501 ctgtttcccc agaagagtat gccgaagatg ccattgcccc ccctagtaaa ttttcaaaca
     1561 ccacagtcgg cggcgcgcaa tttatcacct gggaaaccga tcgtgaattc gatggcttta
     1621 agaatagccc atatatttat tatgtattgc ctatagatca acacggatac ttaacatttt
     1681 tatttaggcc cacgatctca gtttttgggc aagacatgat cgccaaacaa gagcaacgca
     1741 ttcagcaaga catcaacaca tttttatctc atataagagt aaagccaatt taaaatcagc
     1801 tccgagccca gcaaaatcaa acttaagtca attcatactt tagcgaatta agtctcaaga
     1861 agagcctgcc ataaaatgga acttgaaaat ctgctgtggc tgaaaaaaaa ttacatctta
     1921 cgcacattaa ttttcataat ttgaatgcga taggcaattt gccttggcgt catacccaat
     1981 aaacgggcgg ctttggcctg cacccagcca gcttgctcca aagccataat taagcgctcg
     2041 cgctcgtcta cggcggggtt taagaggtcg gcgctgttta ggctgggggg ggttatgtgg
     2101 ttgttgtagc tgggggcgtt gtgttgcgag gggtgggggg agcttggggt gtagaagctg
     2161 ctggcgaggg tgatgacatc cgaatcaatg gtgccctctt cactcatcac cgctgcgcgc
     2221 tctaaagaat tttctaattc ccgtacattg cccggccagt cgtgttgcat taataaccgc
     2281 aatgcactgt cggtaaggcg tagttcgcgg ccttggtgtt tggcaatttt acttaataaa
     2341 aattgcgata attcggggat gtccacaatg cgctcgcgca gcggcggcag gcggatggcc
     2401 atgacgttaa ggcggtaaaa taaatcctcc cgaaagcggc ccttatccac ttccacttct
     2461 aaattgcggt tggtggcggt aacaatgcgc acatcaactt taatggtttg cccgccgcct
     2521 acccgctcta attcgccctc ttgtaatacg cgcaataatt ttgcttggaa catgggcgag
     2581 atttcgccga tttcgtctaa aaataaagtg ccgccatcgg cctgttcaaa gcggcctttg
     2641 cgctgtttca ccgcattggt aaaggcacct ttttcatggc cgaataattc cgactctaat
     2701 aatgtctccg gcaaagaggc acaatttaag cgcacaaatt gattgtgggc gcggggggaa
     2761 ttgtagtgaa tggcattggc gattaattct ttacccgtgc cggattcccc caaaattaat
     2821 accgtgctgt cccatttggc caccctgcgc acctgatcga atacctgccg cataccctcg
     2881 gagcggccca ccaccatgtt gtcaaaaccg tatttagcgc gcacttcccg gcgcaattca
     2941 tcgcgctcgc tggcgatggt gcggcctttt tccatattca ccaataaacg cacggtttgc
     3001 gataataaat tggccaccgc ttccataaat gtagtgcgct cgcttaagta gtcatcggcg
     3061 gccacatccg gctgcgctgc taatacacca atggcagcgc catcgccatc gcgaatgggt
     3121 atagcaataa agggtaattc caaatcgtac agggataaac gatcgagaaa acgcggctcg
     3181 gaggaaatgc gcgcaagcac aatcggttgg ttgtgttgca gtatgcgccc gagcacacct
     3241 tcaccagctc tatagcgcac cgaggcggtg gatttactga ggttttcatc ttcgctgtac
     3301 acagccccca catgtaacac tgtgtgcatg gggtctgcca gggtaattaa accgtgtaat
     3361 aaacgggcgt cgttgtgcaa cacacttaaa atacccgtga gggtatcttc gatatttttt
     3421 tcccaactta atttatgggc aattttgcac agggcggaaa attgccgctc caataactcg
     3481 ctgcggctgc cgatccgact ggccggtttg gtaaaactgg aaggtctcat agggttgtct
     3541 cgttaggatc ttccggccga ggtcagcggt aggtgtacca ccacacggca gccttcacgg
     3601 tagcttggat ctataaatac agtgcctgcg tgatcggcga caatttcctg caccatggat
     3661 aaccccatgc cccggcaact gcgccccggc ggtttagtgc tgtaaaacgg ctcaaacact
     3721 tttacgatca actccggttt aatgccagaa cccgtgtcgg tgatctcaca gatcacccgg
     3781 tctttttcgc cgtgggtgat aatgcttaat tcccgataat caattttgcg atcgctcata
     3841 gcttcaatgg cattgtcaat taactgcttg aacatgccga gcaagcgctg ctcccggcct
     3901 attaccgccg gtaaatataa agacggtttc cactcgatgg taatgccttg ggataataag
     3961 gacacagtgg ataacgcaat ggcatcgcgt attaattggt taatattgac cggtttttta
     4021 gcctcttgca gcggcggcgg tagggcggca tttaaattat ccagcgcctg ttggccggca
     4081 ttaatcgcct cttctaaagc gttcagcaca aagtcatccc ggttggccac c
//
LOCUS       DAOQGS010000372         4117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11810_length_4117_cov_4.602413, whole genome shotgun sequence.
ACCESSION   DAOQGS010000372 DAOQGS010000000
VERSION     DAOQGS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11810_length_4117_cov_4.602413"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKZ52_13065"
     CDS             <1..445
                     /locus_tag="PKZ52_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNG60748.1"
                     /translation="LEIEKQVMLQVLDNCWKEQLASMDHLRQGINLRSYAQRNPKQEY
                     KRESFELFQTLLNNLKHEVTRVLFRVEPITPEQMEAMERAQAAEAERARRQQQLQHDQ
                     VSGFTGEVVQQPQVALTRRGAKVGRNDPCPCGSGLKYKACHGKLA"
     gene            458..1678
                     /gene="argJ"
                     /locus_tag="PKZ52_13070"
     CDS             458..1678
                     /gene="argJ"
                     /locus_tag="PKZ52_13070"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNG60749.1"
                     /translation="MAVGEASWPVMHPISGVRLGVAQAGVKYAGRNDLLVIAIEEGAS
                     VAGVFTQSAFAAAPVLIGREHIANGDIRYLVVNSGNANACTGERGMNDARATCRAVAE
                     LTDVKPHQVIPFSTGVIGEYLPIEKIIGAIPQAIASGLEDNWALAGRTIMTTDTRPKG
                     ASVQFEFDGETISVTGIAKGAGMIRPNMATMLAYIATDAVCAPHVLEAIVKKATDKSF
                     NRITIDGDTSTNDACILIASGQAGLPMLTSTHGELYEKLLAAVTDVQQQLAQAIVKDG
                     EGATKFVAVKVEQGTNSHECLQIAYAIAHSPLIKTALYASDPNWGRIVAAIGYAGVKN
                     LDARLVNVWLDDVLIVANGGRAASYTEEAGAAIVSQAEFTIRVALGRGDAQEQVWTCD
                     LSHEYVTINAEYRS"
     gene            1687..2700
                     /locus_tag="PKZ52_13075"
     CDS             1687..2700
                     /locus_tag="PKZ52_13075"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNG60750.1"
                     /translation="MSALAVVVGVIVNGAGEILLAKRAAHQHQGGLWEFPGGKLEASE
                     APLAGLWRELQEELGISVVKAEPLIQIHHQYPDKSVLLDVYRVTQFDGVAQGREGQPL
                     VWIKPSELNNYPFPAANRPIVQALMLPQQLCITGAAATPAIWSERFAQSLQPNTWMLA
                     RPQNEEQLSWPWPQLIIDAFSLCQRHNTRLLLHSSLLLKMAPGFNGLGPEEQRNLLQQ
                     YCHGVHLTAAAAADQLLCIPTDFPLGMSCHTEQELAKAHALNCHYALLSPALPTQTHA
                     DAAPLGWSEFTRLCRLAKVPVFALGGIDARHRGLAQSLGAQGVAGIRGFWGINSTGAG
                     DNT"
     gene            2727..3449
                     /locus_tag="PKZ52_13080"
     CDS             2727..3449
                     /locus_tag="PKZ52_13080"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNG60751.1"
                     /translation="MITQLRKCVHLSHYRAAGLLGAAVLLCSSAALALSLADLTEKDA
                     NAGLKSAMEQGSVAAVAKLGAANGFLNNDKVRIKLPGKFDKVRSVLQLTGQGQKIDDL
                     ELAMNRAAESAVPMAKPLLISAVKSMTFNDAKNILAGGETSVTDFFREKTAASLAVKF
                     LPLVKGVTDKAKLAQSYNSVMSKAQTLGLASDQEATVEAYVTKKAAEGLYVMIAEEEK
                     AIRRDPVGTGSKIIGKVFGALK"
     gene            3870..>4117
                     /locus_tag="PKZ52_13085"
     CDS             3870..>4117
                     /locus_tag="PKZ52_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNG60752.1"
                     /translation="MRYLVFASAFVAGFVVMVLEMLGGRIIAPWFGGDIYVWGSIITV
                     FMLALSLGYLIGGRWSLHNASLRRYGLIFLGAGLLILPV"
BASE COUNT          944 a         1106 c         1141 g          926 t
ORIGIN      
        1 gttagaaatt gaaaaacaag tgatgctaca agtgcttgat aactgctgga aagagcagct
       61 ggcgagtatg gatcatttgc gtcaggggat taacttgcgt tcctacgcgc aacgtaatcc
      121 taaacaagaa tacaagcgcg aatcctttga gctgtttcaa accctgctta acaatcttaa
      181 acacgaagtg acccgcgtac tcttccgggt ggaacctatt acccctgagc agatggaagc
      241 catggagcgc gcccaagcgg ccgaggctga gcgcgcccgc cgccagcaac agctgcagca
      301 cgatcaggtc tccggtttca ccggcgaagt cgttcaacag ccgcaagtgg cacttactcg
      361 gcgcggcgcc aaagtgggcc gcaacgatcc gtgcccctgt ggttctggat tgaaatacaa
      421 agcatgccac ggcaagttgg cgtaagaggg tagacccatg gcagtaggtg aggccagctg
      481 gccagtaatg catcccatca gcggcgtgcg cttgggggtg gctcaggcgg gcgtgaaata
      541 cgccggtcgc aacgacctgt tggtgatcgc cattgaagag ggcgcaagcg tggccggggt
      601 atttacccag agcgccttcg ccgccgcccc cgtgttaatc ggccgggagc acatagccaa
      661 tggcgatatt cgctacctgg tggtgaactc cggcaatgcc aatgcctgca ccggcgagcg
      721 cggtatgaat gacgcccgcg ccacctgccg ggcggtggcg gaactcaccg atgtaaaacc
      781 ccaccaagtg attccctttt caaccggagt gattggtgaa tacctgccca ttgaaaaaat
      841 cattggggct ataccccagg ccatcgcaag cggcttggaa gacaactggg ccttagctgg
      901 ccgcaccatc atgaccaccg atacccgccc caagggcgcc tcggtgcagt ttgagtttga
      961 tggcgagacc atcagcgtga ccggcatcgc caagggtgcc ggaatgattc ggcccaatat
     1021 ggccaccatg ctggcctata tagccaccga tgcggtgtgt gccccccatg tgctggaggc
     1081 catagttaaa aaagctaccg ataaatcctt taaccgcatc accattgatg gcgatacctc
     1141 caccaacgac gcctgtattt taattgccag cggccaagcg ggcctgccca tgctcaccag
     1201 cacccacggt gagttgtatg aaaaactgct ggcggcggta accgatgtgc agcagcagct
     1261 ggctcaagcc atagtgaaag acggcgaagg cgccaccaag tttgtggcgg taaaagttga
     1321 gcagggcact aatagccatg agtgtttgca aatcgcctac gccatcgccc attcccccct
     1381 catcaaaacc gccctctacg ccagcgaccc caactggggc cgtattgtcg cggccatagg
     1441 ctacgcaggg gtgaagaacc tcgatgcccg cttggtgaat gtgtggctgg atgatgtgtt
     1501 aatcgtcgcc aacggcggcc gcgccgccag ttacaccgag gaagccggtg ccgcgattgt
     1561 gagccaagcc gaatttacca ttcgtgtggc cctaggccgg ggggatgccc aagagcaggt
     1621 gtggacctgc gatctctccc acgaatacgt caccatcaac gccgaatacc gctcctaagc
     1681 tgccccgtgt ccgcactggc cgttgttgta ggggtaatcg ttaacggcgc gggggagatt
     1741 ctgctggcca aacgagcggc ccaccagcac cagggcggtc tatgggaatt ccccggcggc
     1801 aagctggagg ccagtgaggc gccgctggct ggtctctggc gggagctgca ggaggagctg
     1861 ggaatctcgg tagttaaggc cgagccctta atccaaatcc accaccagta cccggataaa
     1921 agcgtgctgc tggatgtgta ccgggtgact caatttgatg gtgttgccca gggccgagag
     1981 gggcaaccgc tggtttggat taagcccagc gaactgaata attacccatt ccccgccgcc
     2041 aaccgtccta ttgtgcaggc gcttatgttg ccgcaacagc tgtgtatcac aggggctgct
     2101 gccactccgg cgatctggtc agagcgtttt gcccagagtc tgcagccgaa tacctggatg
     2161 ttggcgcgcc cccaaaacga agaacagctc agctggcctt ggccacaatt aattatcgat
     2221 gcctttagct tgtgtcagcg ccacaacacg cggctacttt tgcacagcag tttattgtta
     2281 aagatggcgc caggctttaa cggccttggg cctgaggagc agcgcaacct tttacaacag
     2341 tattgccacg gcgtgcatct aaccgccgct gcagctgcgg atcagctgct gtgcatacct
     2401 acagatttcc ccctgggcat gtcttgccat actgagcagg agcttgctaa ggcacatgca
     2461 ctcaactgcc actacgcgct gctttcaccc gcgttgccca ctcaaaccca tgcagatgcg
     2521 gcgcccctcg gctggagtga atttacccgc ctatgccgcc tggcaaaagt gcctgtattt
     2581 gccttgggtg gcattgatgc tcggcacagg gggctggcac aaagcttggg tgcgcaaggc
     2641 gttgccggta tacgcggctt ttggggaatt aattcaaccg gggctggcga taacacctga
     2701 cttatttatt cacgcgagga agaaccatga tcacgcaact gagaaaatgt gtgcacttaa
     2761 gccattaccg ggcggcaggt ttgctgggcg ccgctgtgtt gttatgttcc tcagccgcac
     2821 tggccttaag ccttgccgat ttaactgaaa aggatgccaa tgccggttta aaaagtgcta
     2881 tggagcaagg ctctgtggcg gcggtggcca aattaggcgc ggccaatgga tttttaaata
     2941 acgacaaggt gcgcattaaa ttgcccggca aattcgacaa ggtgcgcagc gtgttgcagc
     3001 ttacaggcca agggcaaaaa attgatgact tggaattagc catgaaccgt gcagcggaaa
     3061 gcgccgtgcc gatggcaaaa cctctgttaa ttagcgccgt aaaatccatg acctttaacg
     3121 atgcgaaaaa tattttggcg ggcggcgaaa cttccgtgac ggattttttt cgcgaaaaaa
     3181 cagcggcgtc tttagcggtg aaatttctcc ctttagtgaa aggcgtaacc gacaaagcta
     3241 aattggccca atcctataat tcggtgatgt ctaaagcgca aacgctcggc ttagcgtccg
     3301 accaagaagc cacggtggaa gcatatgtca ctaaaaaggc ggcagaaggc ttatatgtga
     3361 tgattgccga agaggaaaaa gccattcgcc gcgacccggt cggcaccggc agtaaaatta
     3421 ttggcaaagt atttggcgcg ttgaaataaa cacacctagg cttcaaggtt tctctaggca
     3481 gtaatttctt aagggcgcct caaaaattca ctgttcgcgc cctcgctatt ttttgtgctc
     3541 agccagtcgt tgcctctaaa cttaccatcg gctgctggcg tcctgcacac gctcgtccgc
     3601 gcaaaaatgc ctagttaaca tcacaaaaat tgaattttcg tcgctgcctt taactctgct
     3661 gctgcaatcg caacaattta ttcttggtgt cctgacagcc tgttgaaata acagttctcc
     3721 agaccattgc ctaaatagtt caaccagcgg ctcgaaacac ttcctacact atcctcgccg
     3781 ttaaactatg cgttattctt cgcagcctgt ggcaatacct gtttgcagtg tacaggcgcc
     3841 tggtgtgtta tttttgagtt gatcaatcta tgcgttattt agtgtttgcc tcagcatttg
     3901 ttgcaggttt tgtggtgatg gtattggaga tgctgggcgg gcgaattatt gcgccctggt
     3961 tcggcggcga tatttatgtg tggggcagta ttatcacggt gtttatgctg gcgctgagct
     4021 tggggtattt aattggcggt cgctggtcac tacacaatgc gagtctacgg cgctatggcc
     4081 taatcttttt aggtgccggg ttattaattt taccggt
//
LOCUS       DAOQGS010000373         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11847_length_4109_cov_5.017267, whole genome shotgun sequence.
ACCESSION   DAOQGS010000373 DAOQGS010000000
VERSION     DAOQGS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11847_length_4109_cov_5.017267"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1460
                     /locus_tag="PKZ52_13090"
     CDS             273..1460
                     /locus_tag="PKZ52_13090"
                     /inference="COORDINATES: protein motif:HMM:NF037817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5610 domain-containing protein"
                     /protein_id="HNG60753.1"
                     /translation="MLTFHSSSVERTGVWLKTTPATPAAASVTTQSLQRPKDNSDTVN
                     ISSQGVARAQALDAQPATNPYANTIVNFIQLQLQKDQADGASKEALSQRLAAGFEGFL
                     KGYEDAYALLGGSGALPDEVNEALAQTKQQVTDAVAKLAEDFGIEAPASATQTDNKNP
                     DSRAQLQKALDQLVDSTRTDNLAAAAANNNGHSALGQTRSLNLQLTTAEGDIIELIAN
                     NTSATAFANSDTEIHGRAQQSSQWSLSVQGDLNDQELSAISDFINQLDGLADEFYQGD
                     LSQAVSFAQNIGFDNTQISAFSLSLKQVDIRRVESTYGGPQSGSSEVKNPQQSRLQLL
                     GQWLDQLDQLRNNSLNNALPEHWLQQLSVQSLAQWYPGNEKENAFLSDYLSADSGSES
                     QSA"
     gene            complement(1855..>4109)
                     /locus_tag="PKZ52_13095"
     CDS             complement(1855..>4109)
                     /locus_tag="PKZ52_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG60754.1"
                     /translation="PRFVNVQPSLFDDWDQLKENAKSLADIVAEQDDAAYNDIWLAQS
                     HQLQLLLNTMREAVLYFDSQGCLKLANNSAQQWLLKSPHTGARLSELFSQWPDAHKQQ
                     EEIFQVVRRGTNTWGSKSALVLGELTYWVSIDKIATTDLGGQVNGVMAVFTDITDTVL
                     KEKALQEGEMRYRAYMSHSNDGIWCYELLAPVDIHLSIDEQAQQIAKQARLAECNERF
                     AQMYGFDSVQAMLGLTLFRPESQSTLDKIRLFIEQGYCFNQEELIFRTRQSESLHLQT
                     NASGIIENDKLIRIWGASHNISSSRHYQARVDFLANHDSLTQLPNRTCLYHFIEKQMV
                     SVPSGQSLALLLIDLDRFKEINDTLGHSFGDHLLRKIGERLSQLFAGSNAQIARLGGD
                     EFAICLPIVEHKDEAMSVAAAVARSIASMGDINGITLAMSASIGVSLYPQQAPDLSTL
                     MRFADVAMYYAKSHYKGVALYDGDIDPHSQQRLELMGALGQAIQAQELVLYFQPKINL
                     QDDQIYAFEALVRWQHPELGLIPPSEFIPLAEMSSLIYPLTRWVLENSISQCQAWRRM
                     GYPLGVAVNLSVRNLMDERIVGDLRKMLVLYNLPADALELEITESMIMHDPERALDVL
                     EQLADLGITLSIDDFGTGYSSLAYLKRLPVRTLKIDYSFVCGMLQDKQDQIIVHSTIN
                     LAHNLGLYVVAEGVESAEVWAELKQLGCDAAQGFYLGIPMPAPDSLAWLSQGSWQPPG
                     AIAKAAASIE"
BASE COUNT         1083 a         1054 c          965 g         1007 t
ORIGIN      
        1 tcggcaattg ctcctgcatt gctctacctc ctgcctccct gcagtcgtcc gctttttttg
       61 ccaaattcgc ctagctagtg cttcgccgcc tacatttttc agaggttccc taggtattta
      121 aaccagctgt taggttcttg agtcacactt taagacaaca aaaagttctc agcagaagct
      181 aaataaaaat caatctccgc cgataaccca atacggggtt gaataaatca actctgcttg
      241 tgttaccgcc cattgtgctg tggagactat atatgctgac ttttcattcg agctccgtgg
      301 aacgcacagg cgtttggtta aaaactaccc ccgcaacccc agcggctgcc agcgttacta
      361 ctcaatcgct gcagagacct aaggataata gcgatacggt aaatatcagc tcccaaggcg
      421 tagcgcgggc gcaggccttg gacgcgcagc ctgctacaaa tccctacgcc aataccattg
      481 tcaattttat tcagttgcaa ctgcaaaaag atcaagccga cggcgccagc aaagaggcct
      541 tatcacaacg cctggcggca ggttttgaag gttttttaaa gggctacgag gatgcctatg
      601 cgctactggg cggcagcggt gctctgcctg atgaggtgaa cgaggccctg gcgcaaacca
      661 agcagcaagt gacggatgca gtggcaaaac ttgccgagga ttttggtata gaagcgcctg
      721 catcggccac acaaactgat aataaaaacc cagacagccg cgctcaatta caaaaggcct
      781 tggatcagct cgtcgattct acccgcaccg ataatctcgc tgccgctgca gctaataaca
      841 acggccacag cgcactgggg caaacccgca gtttaaattt acaattaacc accgccgaag
      901 gcgatattat tgaattaatt gccaacaata cttccgcaac cgcgtttgcc aattccgata
      961 ctgaaatcca cggccgcgca caacaatcca gccagtggag cctatctgtg cagggcgatt
     1021 taaatgacca agagctcagt gccattagtg attttattaa tcaattggac ggcctggccg
     1081 atgagtttta ccagggagat ttatcccaag ccgtaagctt tgcgcagaat atcggttttg
     1141 ataatacaca aattagcgct ttttcattga gcctgaaaca agttgatatt cgccgcgtag
     1201 aatccaccta tggcggcccc caatccggta gcagcgaagt taaaaatcca cagcaatcgc
     1261 gcctgcaact acttgggcaa tggctggatc aattggatca attgcgcaat aacagcctta
     1321 ataacgccct gccagaacat tggttacagc aactaagcgt gcaatccctc gcccagtggt
     1381 atccgggcaa tgagaaagaa aatgcttttt taagcgatta tttatcggct gatagcggct
     1441 ctgaaagcca gtcagcctag gctgattgct cgctccactg gagcggcaaa ttgaggggat
     1501 ttagccatgc ggcacgaccc taaaagcgca gttaaaccct gctgcagttt ttaagatata
     1561 caattcgcgg tgtagcagtg atgaatgcag gtttattgca gaccgtcaaa aagagggacc
     1621 tttttgacga gcctacacgg atgtatttac ggcgtgtctg caataaacct ctattcgcca
     1681 ctgcgggcgg aatcttgaga gcgcgcagct aaagcacggt ttctagtcag tgtgagaaca
     1741 cgtccagtta cacttattat ccgaaaccgc taggtattaa atagttacca gcttgtcagg
     1801 atatattcgc agtgcgcctg aattgcccgc attctttttt aagcggatga acgcctattc
     1861 gatactcgct gcagctttcg ctatagcgcc aggtggctgc caactgccct ggctcaacca
     1921 ggcaaggctg tcgggggccg gcataggaat gcccagataa aacccctggg cggcgtcgca
     1981 gcctaattgt tttagctccg cccaaacttc agcactttca acaccttctg ccaccacata
     2041 caggcccaaa ttatgggcaa ggtttatggt tgaatgcacg ataatttgat cctgtttatc
     2101 ctgcaacata ccgcagacaa acgaataatc aatttttaag gtgcgcacag gcaggcgctt
     2161 taaataggcc agggaagaat aaccggtgcc aaaatcatca atcgacagag taatacctaa
     2221 atccgccaat tgctctaata catccaatgc ccgctccggg tcgtgcatta tcatgctttc
     2281 agtaatttct aattccagcg cgtcggcggg caagttgtac agcaccaaca ttttgcgcaa
     2341 atcccccact atgcgctcgt ccattaaatt gcgcaccgaa agattcaccg caacccccaa
     2401 gggataaccc atgcggcgcc aggcttggca ttggctgatg ctgttttcca acacccagcg
     2461 agtcaaggga taaataagcg acgacatttc ggctaaggga ataaattccg atgggggaat
     2521 taaccccagc tcaggatgtt gccagcgcac caaggcttca aaggcataaa tttgatcgtc
     2581 ttgtaaattt attttcggct gaaaatagag cactaattcc tgcgcctgaa tggcctgccc
     2641 aagggcgccc attaattcca agcgctgctg ggagtgagga tctatatcgc catcgtacag
     2701 cgccacacct ttgtaatggc ttttagcgta atacatagcc acatcggcaa agcgcataag
     2761 ggtgcttaaa tccggcgcct gttgagggta gagcgatacc ccaatactgg cgctcatggc
     2821 caaggtaatt ccgtttatat cacccataga agcgatggag cgggcaactg ccgcagccac
     2881 cgacatagcc tcgtctttgt gctccacaat gggtaaacaa atggcaaatt catcgccgcc
     2941 taaacgggca atttgtgcat tgctaccggc aaataattgg cttaaacgct cgccaatttt
     3001 acgcagtaaa tgatcgccaa aactgtggcc gagggtatcg ttaatttctt taaagcggtc
     3061 taaatcaatt aataacagcg caaggctttg gccgctgggc acgctcacca tttgtttttc
     3121 aataaaatgg tacaggcagg tgcgattggg cagctgggtg aggctgtcgt ggttggccag
     3181 aaaatccacc cgcgcctggt aatggcggct gctggaaata ttatgggagg cgccccagat
     3241 acgaattaat ttatcgttct caataatgcc tgaggcattg gtctgcaggt gcaaactctc
     3301 gctctgccga gtgcggaaga tcaattcttc ctgattaaaa caataccctt gttcgataaa
     3361 caaacgtatt ttatccaggg ttgattggga ttcaggccga aatagcgtta agcccaacat
     3421 tgcctgcaca gaatcaaagc catacatctg ggcaaaacgc tcattgcact ccgctaagcg
     3481 ggcctgtttg gcaatttgtt gggcctgctc gtcaatactt aaatgaatat ccaccggggc
     3541 gagtaattcg tagcaccaaa tgccatcgtt ggagtggctc atataggccc gataacgcat
     3601 ttcaccttct tgcagggcct tttcttttaa taccgtatcg gtgatatcgg taaataccgc
     3661 cataacacca ttgacctggc cgcctaaatc cgtagtggca attttgtcga tgctcaccca
     3721 ataggtcagc tcacctagca ccagtgccga tttagagccc caggtgttag tgccccggcg
     3781 caccacctgg aatatttctt cctgttgttt gtgcgcatcg ggccattggc taaagagttc
     3841 gcttaaacgc gcgccggtgt gaggtgattt aagcagccat tgttgggcgc tgttattggc
     3901 taattttaag cagccttgcg aatcaaaata caacacggcc tcgcgcatgg tgttgagcaa
     3961 tagctgtaac tgatggctct gcgcaagcca tatgtcgttg taggcagcat catcctgctc
     4021 agccacaatg tccgcgaggg atttggcgtt ttcctttagt tggtcccaat catcaaataa
     4081 actcggttga acattaacaa aacggggtt
//
LOCUS       DAOQGS010000374         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11872_length_4103_cov_4.516551, whole genome shotgun sequence.
ACCESSION   DAOQGS010000374 DAOQGS010000000
VERSION     DAOQGS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11872_length_4103_cov_4.516551"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2150
                     /locus_tag="PKZ52_13100"
     CDS             <1..2150
                     /locus_tag="PKZ52_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNG60755.1"
                     /translation="FVKGLQGDDPHYFKTDATAKHFAVHSGPEFNRHEFNATPSERDL
                     YETYLPAFKALVQEGKVAAVMGAYNRVNGESASASKRLLQDILRGEWGFKGYVVSDCD
                     SIEDIYLHHKIVKTAAEAAALGIKTGMDLNCGKTYAALLEAVNNNLITEAQIDTALKR
                     LMYARLRLGLFDPPAQVPWNNLPNTLLQSAEHNQLARRAAQKSMVLLKNNGLLPLKKD
                     LKAIAVIGPTADEPVALLGNYYGTPASPVTFLKGIQQAVSPSTQVYYARGADLVDGRQ
                     EPRAAPIIASKYLRPHKNSAEQGLTGEYFRGKDLAAKPVLTRTDSTIAFRWDRNSPTD
                     ELVARGELNRSANLDKDNFSIRWRGQLIAPITGEYEIHLTANDGARLTLGGKTLIDAW
                     QATDHVINHTAKINLTAGEPQDIQLDYFEDIRDAEIRLAWRLPGAKAPLQEALEAAAK
                     AEVIIVAAGLTSEVEGEEMKVNYPGFNGGDRTDLRLPKPQQELLTALNALGKPIVLVL
                     TGGGALAVDWAQENIPAILMAWYPGQAGGAALADILFGDVSPSGRLPVTFYSSGNKLP
                     DFQDYSMQGRTYRYFAGTPLYPFGHGLGYSQFSYSQLKTAPKLRGNQSTTITATIKNT
                     GNRPATEVVQLYVRPIKPGPQDALKQLRGINTVDLAAGEEKTLHFTLAGLNDFAVYDP
                     AKHSFVVKPGDYEVQLGASSADIRLRKTIRVSP"
     gene            2940..3884
                     /locus_tag="PKZ52_13105"
     CDS             2940..3884
                     /locus_tag="PKZ52_13105"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNG60756.1"
                     /translation="MQKTLLAMGIALLSASMAGCGGGGASSSSSSSSSSSSSSSSSSS
                     TSSSSSSSSSSSTSSSSSGTVHATCPSTNAAAFQNFATVVPGKIESENFDLTGFMDST
                     STNEGGAYRTDTAVDIKAISGGYAVGWITAGEYLEYSLYVQTPGTYDVYLRSGAVAAG
                     RTISITQCDKSLLRDIKIPVVANWGEYKTWKVGSVQLTAGIQKIRVTAGTMDSLDLDW
                     INIGTYTGTIDPPDPVSTSSSSSSSSSSSSSSSSSSGGANGSALFASKCQSCHSASSL
                     ANHSATQIKNAINSVGRMSSLKSMSDADISAIATYLKR"
BASE COUNT         1090 a         1126 c          939 g          948 t
ORIGIN      
        1 catttgtaaa aggcctgcag ggggatgacc cgcattattt taaaaccgac gccaccgcca
       61 aacattttgc cgtgcacagc gggccggaat ttaatcgcca cgaatttaat gccaccccca
      121 gcgagcggga tttatacgaa acctatttac ccgcctttaa agccttggtg caagaaggca
      181 aggttgcagc ggtaatgggt gcttacaacc gggtcaatgg cgaatccgcc tccgccagta
      241 aacgcctatt acaggatatt ttgcgcggcg aatggggttt taaaggttat gtggtttccg
      301 attgcgactc tatagaagat atttatttac accataaaat tgtcaaaacc gccgccgaag
      361 ccgccgccct cggcattaaa actggcatgg atttaaattg cggcaaaacc tacgccgcgc
      421 tgttagaggc ggtaaacaat aatttaatta ccgaggcgca aattgatact gcattaaagc
      481 gcttaatgta cgcccgcctg cgcttgggat tattcgatcc accagcccaa gtgccttgga
      541 ataatttacc caacacatta ctgcaatcgg ctgagcacaa ccaattagcg cgccgggccg
      601 cgcaaaaatc catggtgctg ctgaaaaata atggcctgtt gccgctgaaa aaagatctta
      661 aagccattgc cgtaattggc cccaccgccg atgagccggt tgcactgcta ggtaattatt
      721 acggtacccc ggcatccccc gttacctttt taaaaggcat ccaacaagcc gtctccccca
      781 gtacccaggt ttattatgcg cggggtgcgg atttagtgga cggcaggcaa gaaccccgag
      841 cggcgcccat tattgcaagc aaatatttgc ggccgcataa aaattctgcc gagcagggtt
      901 taaccggcga atattttcgc ggtaaagatt tagcggctaa acctgtactc actcgcacag
      961 actcaactat cgcctttcgc tgggatcgca attcccccac cgatgaatta gtcgcacgcg
     1021 gtgaattaaa ccgcagcgca aaccttgata aagacaattt tagtattcgc tggcgcgggc
     1081 aattaattgc gccgattacg ggcgaatatg aaattcacct caccgccaac gacggcgccc
     1141 gcttaacact gggcggcaaa accctgattg atgcctggca agccaccgac cacgtcatta
     1201 accacaccgc taaaataaac ctaaccgcag gcgagcccca ggatattcaa ctggattatt
     1261 ttgaagatat ccgcgatgca gaaattcgcc tcgcctggcg cttacccggc gctaaagcac
     1321 ccttgcaaga agcgctggag gccgccgcga aagccgaggt aattattgtg gcggcaggcc
     1381 ttactagtga ggtggaaggc gaagaaatga aagtgaatta cccgggtttt aatggcggcg
     1441 accgcacgga tttgcgcctg cccaaacccc agcaggaatt attaaccgcc ctcaacgcgc
     1501 tgggtaaacc catagtattg gtattaaccg gcggcggcgc cttggcggtg gattgggcgc
     1561 aggagaatat cccggctatt ttaatggctt ggtatccggg gcaagcaggc ggcgctgcat
     1621 tggcggatat tttatttggc gatgtcagcc cgtccggccg cctgcccgtt actttttata
     1681 gcagcggcaa taagctgcca gattttcaag attattccat gcaggggcgc acctatcgat
     1741 attttgccgg cacccccttg taccccttcg gccatggctt aggctacagc caatttagtt
     1801 atagccaatt aaaaacagca cccaaactgc gcggcaatca atccacaaca attaccgcaa
     1861 ccattaaaaa taccggcaac cggcccgcta cagaggtagt gcaattatat gtacgcccta
     1921 ttaaaccagg cccccaggat gcgttaaaac aattgcgcgg cataaatacg gtggatttag
     1981 cagcggggga agaaaaaacc ctgcacttta ctcttgcggg gctaaacgat tttgcagttt
     2041 acgacccagc caagcacagc ttcgtcgtaa aacctggtga ctatgaagtg caactggggg
     2101 catccagcgc cgatattcgc ctgcgcaaga ctattcgcgt ctccccctaa caatccggtt
     2161 aaataccacc tgccctattt acagcagact ctttaagggt tggcagcctt tttttattat
     2221 cggcagggcc taaaattccc ctcctgtgga ggggtgcccc gaaggggtgg ggtggtagat
     2281 cgcttcgttt ttttgcagcc cgctgtaatt agagatttgc gtttcacctg gaataatagt
     2341 cacctgaaac aaaagccacc tgaaataaaa ccgggtcctc caccgcaacc accccggcag
     2401 gcaagcctgc cacccctcca ggggagggga attttagctg gcgctagctg caaaattcct
     2461 cgtaatcatc tcgaaaatta actcacctca tccaagccaa ttcttattcg ccccacttaa
     2521 gaattattac catcggaaaa ttcaattctt ttcgttttgc attcaagtcc gctccgtgta
     2581 aaaaagacgc tatttcccag ccaaatagtt aaaaaacata tatggtaaaa aacaaaaaca
     2641 tggggcgata aatacctatg tttaaatcgt caaaaaataa taagtgatat taattgactc
     2701 atagatcaca cattcgctgc agcgtgctcg gcaaagttag gcattggcac cacgacggcc
     2761 gctcatttgt gtttacatat gggagtgaat ttctcacatg gggctggacg acctagatgt
     2821 attagatgca caccagatgc gagggtttat gcgcgcaaac accaggtgcc acaggcgtgt
     2881 tttcgccagc atagctaccc agccttttcc ctcactctta tttttcaggc ggaattatta
     2941 tgcaaaaaac tcttttagcc atgggtatcg ccctcttgtc agcctctatg gcaggctgcg
     3001 gtggcggcgg cgccagcagc agttcgtcct ccagtagttc atccagcagc agctcatcct
     3061 ccagcagttc aacttccagc agcagctcct cgagcagctc ttcttccacc agctcatcca
     3121 gcagcggcac tgtgcacgcc acctgcccca gtactaacgc tgcagccttc caaaatttcg
     3181 ccacggttgt gccaggcaaa attgaatctg aaaatttcga tttaaccggc tttatggatt
     3241 ccacttccac caatgaaggc ggcgcctatc gcaccgacac tgcagtggat attaaagcca
     3301 tctccggcgg ttacgcagtg ggctggatta ccgcaggcga atatttagag tactcactct
     3361 atgtccaaac ccccggcacc tacgacgttt atttacgctc cggcgccgta gccgctggcc
     3421 gcaccatatc cattacccag tgtgataaaa gcctgttaag agatattaaa attccagtgg
     3481 ttgccaattg gggcgaatat aaaacctgga aagtaggcag cgtgcagtta accgcaggca
     3541 tccaaaaaat ccgtgtaacc gcaggcacta tggattcgtt ggatcttgat tggatcaaca
     3601 tcggcaccta caccggcacc attgatccac cagacccagt ctccacctcc agcagctcgt
     3661 caagctctag ctcatcaagc tccagctctt caagttcaag cggtggcgcc aatggctcag
     3721 ccttgtttgc cagcaaatgc caaagctgcc acagcgcctc aagcctggcc aaccactctg
     3781 ccacgcaaat taaaaatgcc attaatagtg taggccgcat gtccagctta aaatctatgt
     3841 ccgatgcgga catcagcgct attgccacct acttaaagcg ttaatccccc tcaaagtaaa
     3901 acagccacct tcgggtggct ttttttggct tggcattttg ggcctggaat aaatatccaa
     3961 aacgagcagt tactacctag acacctgcaa tgaatacaca tcatttcaga cacgccgtaa
     4021 atacatccgt gtaggctcgt caaaaagggg cagattcaac tccgaagtgc atcacagccc
     4081 ccggctagaa aaagaagcca gct
//
LOCUS       DAOQGS010000375         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_11886_length_4100_cov_4.302596, whole genome shotgun sequence.
ACCESSION   DAOQGS010000375 DAOQGS010000000
VERSION     DAOQGS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11886_length_4100_cov_4.302596"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKZ52_13110"
     CDS             <1..646
                     /locus_tag="PKZ52_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG60757.1"
                     /translation="GLFLASIPLLLIITAFLSSSGISADMFAGEREKRTLEALVITSC
                     PSHAFILGKWLCAWGLTAIAITWQLLLLSLAFAVLPLSELGIRVSVSLGDWLIIWLTL
                     LNLGALACGLQVLISLHARNFKDAQMLISLMAAIPVVTLGYTLFTPDFYSAWFNWLPI
                     LSHQLIIKTLLTGQSWQLWAFAQSLLASLIPWCLIMLLAQSRLRSARVIYGRST"
     gene            complement(1025..1786)
                     /locus_tag="PKZ52_13115"
     CDS             complement(1025..1786)
                     /locus_tag="PKZ52_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HNG60758.1"
                     /translation="MSPESFPLDAYLARIGLAARPSTDAAGLAALMRAQLFTVPFENT
                     YVQAGLGVSLEPAAIVDKIIYRRRGGYCYEVNGLFAWALQALGFQVRLVGARPMFYGR
                     RWAKTHMGLVVDLPDGTYFCDTGFGRFGLRAPLPLVSSPNPVQQDWDQFRIHKEGHEW
                     IVEALNNGEWESQFSFDEFPAEWVDFMPANFFNSHSPDTIFVQKLLIIQHQPDARVIL
                     LDDVLKTIREGGTSEEKLNEPVERRVERFGLSWPV"
     gene            1953..3578
                     /locus_tag="PKZ52_13120"
     CDS             1953..3578
                     /locus_tag="PKZ52_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60759.1"
                     /translation="MGQFNVECGTLDGSVTASVKATQFNSPPRYLSAMKSLRPLLLSL
                     ALTASLPVFASSSSVFETNFDGDGKNIPFTLLYGASIEANDGRGNSAYLKCAQGGSTL
                     ISKQPINEMGVLELWVKPTAPDTHYEMTFYVRAKDKDENSWVKIGAIDSEKNDAEYTA
                     KRVSIDEAGSNYLKIEINVSSGSIAIDDIAIDKIGLAIALRKSQLETQKNLQAQFSKD
                     EDAKILTESIVRLGEAYISQIELQRRYLESSAGITATLQLALATSQRNQMANPLAYQT
                     FGGIVKDVKSLSSPIQSQRVDSLLKPFGDIATATLNVVSGGVYPIFAETFKSIVAASF
                     ERNNYANLDIPKDLRKYAEKNGPQVYTKTETFLNEIKNDLGNITALDKDLSGIQKDVD
                     SFRKDLEKNLREYLVTGGMEKNNDSLNRIMSKDPKDREGAIKVVRDYFASQAAFFQNG
                     TKNNAQLLQFLLVANSKIEKVQEFDERFNQITSSVLTFYSKFEKSIEPERNPFSTDAD
                     KKTWENSAKSAREHVKKSRAAFESAYINYVTGQ"
     gene            3769..>4100
                     /locus_tag="PKZ52_13125"
     CDS             3769..>4100
                     /locus_tag="PKZ52_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNG60760.1"
                     /translation="MQLHNATTTQLTQWEADLAAAYETIKAQKLNLDLTRGKPSSEQL
                     DLSNALDGILRGEYTSPTGVDTRNYGGLDGIPEARALGSWLLSVPEEQVYCGGNSSLT
                     LMHQAVMAA"
BASE COUNT         1058 a         1105 c          962 g          975 t
ORIGIN      
        1 cgggttattt ctcgcctcta ttcccttatt gctaattatc accgcctttt taagcagctc
       61 cggtatcagc gccgatatgt tcgccggtga acgggaaaaa cgcacccttg aagccttggt
      121 gattacctcc tgcccttccc acgcatttat actgggaaaa tggctgtgcg cctggggcct
      181 taccgccatt gccattacct ggcaactact gttgttaagc ctcgcctttg cagtattgcc
      241 gctgagtgaa ttgggtattc gcgtaagcgt aagccttggc gattggctga taatttggtt
      301 aacgctgtta aatctcggcg ccctagcctg cggcttgcag gtattaattt ccttacacgc
      361 ccgcaatttt aaagacgccc agatgcttat tagcctgatg gctgcaattc ccgtagtcac
      421 cctcggctac actttgttta caccggactt ttattcggcc tggtttaatt ggctgcccat
      481 tcttagccac caattaatta tcaaaaccct tttaacgggg caatcctggc aattatgggc
      541 ttttgcccaa agcctgttgg caagcctaat tccctggtgc ctaattatgc tgctcgccca
      601 gtcgcgcctg cgctcagcgc gggttattta tggccgcagc acttaattat tcagcatttt
      661 ttattatctt ctccatgggg gaggcaaatt gaggggaatt tagccaagcg gcataattgt
      721 gaaaacgccg ttgaaatttt ttggacagtc gatcgcggtg cagcaggggt gaatacaggt
      781 tattgcagac cgtcaaaaag agggaccttt ttgacgagcc tacttaggat gtattcacgg
      841 cgtgtctgaa ataacctgta ttcatcattg cggatgcaca gcactcaagc gctcgcttga
      901 cggtttcaag tcgacacaag gttcacctcc aaccagaata atcacgtcca accagaataa
      961 atagatactc aatagatccg ttttcatttt gctaccgagc agccaaaagt ctcttctgca
     1021 acactcaaac cggccaggac aaaccaaagc gctccactcg ccgctctacc ggttcattta
     1081 gtttctcttc gcttgtgcca ccttcgcgaa tggtttttaa cacatcatcc aaaaggatga
     1141 cgcgcgcatc cggctggtgc tgaataatta aaagtttttg cacaaaaata gtatctgggc
     1201 tgtggctgtt aaagaaattt gcgggcataa aatccaccca ttcagccggg aattcatcaa
     1261 agctaaattg gctctcccac tcgccattat taagtgcttc tacaatccat tcatgacctt
     1321 ctttgtgaat ccgaaattga tcccaatcct gctgcacagg gtttgggcta ctcaccaagg
     1381 gcaagggggc gcgcaggccg aagcggccga atccggtgtc acaaaagtag gttccatcgg
     1441 gtaaatccac aactaggccc atatgggttt tcgcccagcg gcggccgtaa aacataggcc
     1501 gcgcgcccac taagcgcacc tggaatccca gcgcctgcaa ggcccaggcg aacaggccat
     1561 ttacttcata acaataacca ccccgacggc ggtaaatgat tttatccaca atggccgcag
     1621 gctctaaact cacccccaag ccagcctgta cataggtgtt ttcaaacggc acggtaaaaa
     1681 gctgcgcccg catcagggcc gcaaggcctg cagcgtccgt gctcggccgg gccgcaaggc
     1741 caatgcgcgc tagataggca tcaagtggga aggattcagg cgacattcag cgctcctttt
     1801 aacaataggg cggggttaag gtgtgttggt tcggcggggc agcgcctggg taaaaatgct
     1861 tgcccaagcc accaccccaa atacagttca caaacgcact ttgacgtttg atcattcata
     1921 cacggggccg taaggcctaa agaggcggca caatggggca atttaacgta gaatgcggca
     1981 cccttgacgg cagtgtaaca gccagtgtca aggccactca atttaactcc ccacctaggt
     2041 acttaagtgc aatgaagtct ttgcgccctc ttttgctcag ccttgcgctc accgcgagcc
     2101 tgccggtttt tgcttcttca tcgtcggtat ttgaaaccaa tttcgatggc gacggcaaaa
     2161 acatcccctt taccctgttg tacggcgcct ccattgaagc caacgatggc cgtggcaata
     2221 gcgcctactt aaaatgcgcc cagggcggct ccaccctcat cagcaagcag ccgattaatg
     2281 aaatgggcgt attggaactc tgggtaaagc ccaccgcacc cgacacccac tatgaaatga
     2341 ccttttatgt gcgcgccaaa gataaagacg aaaatagctg ggtaaaaatt ggcgccattg
     2401 actctgaaaa aaacgatgcg gaatacaccg ccaaacgggt ctccattgat gaggcgggca
     2461 gtaattattt aaaaatcgaa attaatgtca gcagcggcag catcgccatt gatgatattg
     2521 ccatcgacaa aattggcctc gccattgcac tgcgcaaaag ccaattagag acgcaaaaaa
     2581 atctgcaggc gcaatttagt aaagatgaag acgcgaaaat tcttaccgaa tccattgtgc
     2641 ggttgggcga ggcctatatc tcgcaaattg aattgcagcg tcgctatttg gaatcctcag
     2701 cgggcatcac cgctactttg cagctggccc ttgccacctc ccagcgcaac caaatggcta
     2761 accctttggc ctaccaaacc ttcggcggca tagttaagga tgtaaaatcc ctctcctccc
     2821 ccatccagtc ccagcgggtt gattccctgt tgaagccctt tggcgatata gccaccgcca
     2881 cgttaaacgt ggttagcggc ggtgtgtacc ccatttttgc cgagacgttt aaatccatcg
     2941 tggcggcatc gtttgaacga aacaattacg ccaatttgga catacccaaa gacctgcgca
     3001 aatacgcgga gaaaaatggc cctcaggtgt acaccaaaac cgaaacattt ttaaacgaaa
     3061 ttaaaaatga tttgggcaat atcaccgcgc tggataaaga cctcagcggt atccaaaaag
     3121 atgtcgatag cttccgcaag gatttagaaa aaaacctgcg ggagtatttg gttaccggcg
     3181 gtatggaaaa aaataacgac agcctcaacc gcattatgag caaagacccg aaagatcgcg
     3241 aaggggcgat taaagtagtg cgggattatt ttgcctcaca ggcggcgttt ttccaaaacg
     3301 gcactaaaaa caatgcgcag ttgctgcagt ttttattagt ggccaacagc aaaattgaaa
     3361 aagtgcagga gtttgacgag cgctttaacc agattacctc ctcggtactc accttttact
     3421 ccaagtttga aaaatccatc gagcctgagc gcaacccgtt tagcaccgat gcggataaaa
     3481 aaacctggga gaacagcgct aagtccgctc gcgagcatgt gaaaaaatcc cgcgccgcgt
     3541 ttgaaagcgc ttacatcaat tatgtaacgg gccaataagc gccctgcttc acaaggccgc
     3601 gcaagcggcc tttttattgc ccgcggcggc cgagtaaacg ctgggtttgc gctgcatcta
     3661 tttgcacaat tgagcgtttt atctacaggc cagatcctct ataatcgcgc gctttttcgc
     3721 tgcccgcctg acgggcctca ctctccccac tgcttcgagg gctgtatttt gcaactgcac
     3781 aacgccacca ctacacaatt aacccaatgg gaagctgacc ttgcagcggc ctatgaaacc
     3841 atcaaagccc aaaaactcaa tttagactta acccgcggca agccttcctc tgagcagttg
     3901 gatctctcca acgcgttaga cggtattttg cgcggcgaat acaccagccc caccggcgtt
     3961 gatacccgca actacggcgg cttggatggc atccccgagg cacgcgcttt gggttcttgg
     4021 ttattaagcg tgcctgaaga gcaggtttat tgtggcggca acagcagctt aaccctgatg
     4081 caccaggcgg taatggcggc
//
LOCUS       DAOQGS010000376         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12001_length_4076_cov_4.150460, whole genome shotgun sequence.
ACCESSION   DAOQGS010000376 DAOQGS010000000
VERSION     DAOQGS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12001_length_4076_cov_4.150460"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="hisS"
                     /locus_tag="PKZ52_13130"
     CDS             complement(<1..793)
                     /gene="hisS"
                     /locus_tag="PKZ52_13130"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNG60761.1"
                     /translation="MTKIQALRGMNDLLPNDSALWQYLEASVADVLGRYGYREIRFPI
                     VESTDLFKRAIGEVTDVVEKEMYTFNDRNDESITLRPEGTAGCVRACEENGLLYNQTQ
                     RLWYQGPMFRYEKPQKGRLRQFHQIGIEAFGMAGPDIDAELLLLTARLWRDLGILEHV
                     RLEINNLGTPQARAEYRSALVAFLNPLAEQLDADSQRRLSTNPLRILDSKHPGTRALL
                     AGAPKLADFLPEANRQHFAQLQAILKQAGLNFTVNPYLVRGLDYYS"
     gene            complement(808..1920)
                     /gene="ispG"
                     /locus_tag="PKZ52_13135"
                     /gene_synonym="gcpE"
     CDS             complement(808..1920)
                     /gene="ispG"
                     /locus_tag="PKZ52_13135"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNG60762.1"
                     /translation="MQCESPIKRRVSRQIMVGNVPVGGGAPISIQSMTNTPTTDVAAT
                     VAQIRRIQEAGADIVRVSVPSMDAAEAFGAIRKQVSIPLVADIHFDYKIALRVADLGV
                     DCLRINPGNIGKEERVRAVIEKARDLNIPIRIGVNAGSLEKELQRKYGEPTPEALVES
                     ALRHVAYLDKYDFQNYKVSVKASDVFMAVAAYRKLATEIEQPLHLGITEAGGLRSGTV
                     KSAVGLGILLMEGIGDTVRISLAADPVEEVKVGWDLLKSLKLRAKGINFIACPSCSRQ
                     NFDVIKTLNELEERLDDIKVSMDVAIIGCIVNGPGEAKEADIGLTGGTPKNLLYVDGE
                     PNTKLENAQLVDDLERIIRAKAQSKLEADQNLIAKA"
     gene            complement(1943..2815)
                     /locus_tag="PKZ52_13140"
     CDS             complement(1943..2815)
                     /locus_tag="PKZ52_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNG60763.1"
                     /translation="MSEVSSEVIESLKTRPGEGLKRLREAAGLSVMDVAEATRITRSR
                     IEALERDDYASAGQSPAHVIGYMRAYSRLLAISADPWLGVLERKLRQEVEDEQKQLLP
                     EPVLVDSHRHYRVLPWVAFMVAGVIVWLVGSDFYTGASPIAQKIEAPELVRLNEMAAK
                     DALAKDIVTPQAPEEKVVDIQPLPKTMPVLDPTSPANSVPTQVINVGTPGAGDALVVA
                     ASDDCWIEVQDRSGKQVFAQLLRTGDNLRLFGVAPFSIKLGNAPAVNLWVNSREIPVQ
                     PENSRRTLRLVAGP"
     gene            complement(2830..3573)
                     /gene="pilW"
                     /locus_tag="PKZ52_13145"
     CDS             complement(2830..3573)
                     /gene="pilW"
                     /locus_tag="PKZ52_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNG60764.1"
                     /translation="MVVLASAVLISGCVSETREGRRNTTQAVETRLELGNRYLADGNR
                     ESARRQFMDVLKMDSGAAEAYLGIARVHEANVEYPEAETNFKKALSKANKESRSSIEM
                     AYGEFLWKRKRYKDAAKHFESAGGDYDFRGRSQGLYWHGRCLSELGDLAKAQGSFQHA
                     INVKPEFGEPYMELADLAFRQRDYINAEQHYEQFTKFAKQNPRSLWLGIRLHRVLGHD
                     DKVASYALVLKNLYGSSDEYLEYQRLIGQ"
     gene            complement(3662..>4076)
                     /locus_tag="PKZ52_13150"
     CDS             complement(3662..>4076)
                     /locus_tag="PKZ52_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNG60765.1"
                     /translation="NKKYPIAMLLASAKRYIQGLPDTHRKITIEYTLIDQLNDRPEHA
                     RELAQLLQDVPVKINLIPFNPFGLVDYKRVSNNALKRFQTILIEAGYTTTIRTTRGDD
                     IDAACGQLAGQVNDRTRRSERYLGRVEAVRLVGGL"
BASE COUNT          872 a         1155 c         1123 g          926 t
ORIGIN      
        1 ccgagtaata atcgaggccg cgcaccaggt aggggttaac cgtaaagtta aggcccgcct
       61 gcttcaatat ggcttgcaac tgggcaaaat gctggcgatt ggcctccggc aaaaagtccg
      121 caagcttcgg cgctccggcc aacagagcgc gggtgccagg gtgtttggaa tctaaaatgc
      181 gcaagggatt ggtgcttaaa cgccgctggg agtcggcatc caactgctcg gccaatggat
      241 tgagaaaagc caccagtgca ctgcggtact ctgcgcgggc ctgaggggtg ccgaggttgt
      301 taatctccaa acgaacatgc tccagaatgc ccagatctcg ccagagccgg gcggtgagca
      361 gcagcagttc tgcatctatg tcggggccag ccataccaaa tgcttcaatg ccgatctggt
      421 ggaactgccg caggcggccc ttttggggct tttcatagcg gaacatgggg ccttggtacc
      481 acaggcgctg ggtttggtta tacagcaggc cgttttcttc acaggcgcgc acgcagcctg
      541 cggtaccttc cgggcgcagg gtaatgctct cgtcgttgcg atcgttaaag gtatacatct
      601 ccttttcgac cacatcggtg acttcaccta tagcgcgctt gaatagatcc gtagattcca
      661 caatcgggaa gcgaatctcc cggtagccgt agcggcccaa tacgtccgcc acactggctt
      721 ccaggtattg ccagagcgcg gaatcgttgg gcaatagatc gttcatgccg cgcagggctt
      781 ggattttagt cactgggaat ctcgtgctta ggctttggcg atgaggtttt gatcggcttc
      841 gagtttgctt tgcgccttgg cgcggatgat gcgctcaagg tcgtccacca actgggcgtt
      901 ttccaacttg gtattgggct cgccatccac atacaacagg tttttagggg tgccgccggt
      961 caggccaata tcggcttctt tggcttcgcc ggggccgttg acgatacagc cgataatggc
     1021 cacgtccatg gacactttga tatcgtccaa gcgctcttcc aactcgttaa gggttttgat
     1081 tacgtcgaag ttctgccgcg agcagctggg gcaggcgata aagttaatgc ccttagcccg
     1141 caatttaagg ctcttaagta aatcccagcc gactttaact tcttccaccg ggtcggcggc
     1201 cagggagata cgcaccgtat cgcctatgcc ttccatcaat aaaatgccca agcccaccgc
     1261 cgacttgacg gtgccggatc ttaagccacc ggcctcggtg atgcccaggt gtaacggctg
     1321 ctcaatctca gtcgccagct tgcggtaggc ggccaccgcc ataaacacat cggaggcctt
     1381 aaccgagact ttgtaatttt ggaaatcgta tttatccagg taagccacat ggcgcagggc
     1441 ggattccact agagcttcag gggtaggctc accgtatttg cgctgcagct ctttttctag
     1501 ggagccggcg ttaacaccta tgcggatggg gatgttgagg tcgcgggctt tttcgatcac
     1561 cgcccgcacc cgctcttctt tgccgatatt gccggggttg atgcgcaggc aatccacccc
     1621 caagtccgcc acccgcaggg caattttgta atcaaaatga atatccgcca ccagcgggat
     1681 agagacttgc ttgcgaatag cgccaaaggc ttcggcggca tccatggagg gaaccgatac
     1741 ccgcactatg tcggcacctg cctcttggat gcggcgaatt tgtgccacgg tggcggccac
     1801 atcggtggtg ggcgtgttgg tcatgctttg gatgctgatg ggcgccccgc cccccacagg
     1861 cacattgccc accataatct gacgggacac tcggcgtttg atgggggact cacactgcat
     1921 aatcgacact aacttaaggc tattacggac cggccaccaa tcgtaaggta cggcgggagt
     1981 tctcaggttg gacgggaatt tcccgcgagt tcacccagag attcacggcg ggagcgttgc
     2041 ccaatttaat gctaaagggg gctacgccaa aaaggcgcag attatccccg gttcgcaaca
     2101 actgcgcaaa tacttgcttg ccggagcgat cctgcacctc aatccaacaa tcatcgctgg
     2161 cggccaccac caaagcatcg ccagcaccgg gggtgcctac attaattacc tgggtgggca
     2221 cgctgttggc cgggctagtg gggtccagca ccggcatggt tttgggtaac ggctgaatat
     2281 cgacaacttt ttcttccggc gcctggggcg tcactatgtc cttggcgagg gcatctttgg
     2341 ccgccatttc attgaggcgc accaattcag gcgcttctat cttctgggcg atgggcgatg
     2401 caccggtata aaaatcgctg cccaccagcc acactatgac gccggcaacc ataaaagcca
     2461 cccagggtaa aacccggtag tggcggtgag aatccaccag cacaggctcc ggcagcaatt
     2521 gcttctgctc gtcttcaacc tcctggcgca gtttgcgctc cagtacccca agccaagggt
     2581 ctgcggaaat cgccaacagg cgcgagtagg cgcgcatata gcctataacg tgggcggggg
     2641 actgcccggc gctggcgtag tcatcccgct ccaaggcttc aatgcgggag cgggtgatgc
     2701 gggtggcctc ggcaacatcc attactgata gcccagcggc ttcccgcaaa cgcttcaagc
     2761 cttcgccggg acgtgttttt aaactttcaa ttacctcaga agatacttca gacataacac
     2821 atcagatggc tactgcccaa ttaaacgttg atattccagg tactcatcgg aggacccata
     2881 gaggtttttt aacaccaggg cgtagcttgc gaccttatcg tcgtgaccta acacccggtg
     2941 caggcgaatg cccaaccata ggctacgggg attttgtttt gcaaatttcg tgaactgttc
     3001 gtagtgctgc tcagcgttaa tgtagtcgcg ctgacgaaag gccagatcag ccagctccat
     3061 atagggctcg ccaaattccg gctttacatt gatcgcatgc tgaaagctgc cttgggcttt
     3121 ggccagatca cccaactcag acaggcagcg gccatgccaa tacaatccct gggaacggcc
     3181 gcggaagtcg taatcgcccc cggcggactc gaagtgtttg gcagcgtcct tgtagcgctt
     3241 gcgtttccag agaaactcac cataagccat ttcgatacta gagcgcgact ccttgttggc
     3301 cttacttaac gctttcttga aattggtctc agcttccggg tactccacat tggcctcatg
     3361 cactcgggcg atacccaaat aggcctcggc agcccctgaa tccattttta gaacgtccat
     3421 aaactggcga cgggccgatt cgcgattgcc atcggccaaa taacgattgc cgagctccag
     3481 gcgcgtttca accgcctgcg ttgtattgcg ccgcccctct ctggtctcgc tgacgcaacc
     3541 gctgattaaa actgcagaag ctaatacaac cattggcaca cagggatacc gcatcgttca
     3601 ctatctcctc aattgaagtt ttagccttag tgtttacctg ttaccgcagg ccgcagcccc
     3661 atcaaagccc gcccaccagc cgcaccgctt ccacccgccc caaatagcgc tcactgcggc
     3721 gggtgcgatc attcacctgc ccggccagct ggccgcaggc ggcgtctatg tcgtcgccgc
     3781 gggtggtgcg aatagtggtg gtgtaaccgg cctcgatcaa aatagtttga aagcgcttta
     3841 atgcattgtt gctgacgcgc ttgtaatcca ccaaaccaaa cgggttaaac ggaatgaggt
     3901 taattttcac cggcacgtcc tgcagcaatt gggcgagttc gcgggcgtgc tcgggccggt
     3961 cgttgagctg atcgattagg gtgtattcaa tggtaatttt gcgatgggta tcgggcaagc
     4021 cttggatata acgcttggcc gaagccaata acatggcgat ggggtatttt ttgttg
//
LOCUS       DAOQGS010000377         4072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12016_length_4072_cov_5.153597, whole genome shotgun sequence.
ACCESSION   DAOQGS010000377 DAOQGS010000000
VERSION     DAOQGS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12016_length_4072_cov_5.153597"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..568)
                     /locus_tag="PKZ52_13155"
     CDS             complement(230..568)
                     /locus_tag="PKZ52_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNG60766.1"
                     /translation="MKLITAVIKPFKLDDVRIALSEVGVQGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLELAVSADMVDKVVEAITKAAQTGKIGDGKIFIQPLEQIVRI
                     RTGETGVEAV"
     gene            complement(790..2229)
                     /locus_tag="PKZ52_13160"
     CDS             complement(790..2229)
                     /locus_tag="PKZ52_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNG60767.1"
                     /translation="MNKNISISSVLKGAAVLWPLAAALLVPGLAIAEEAAVAPPVPQK
                     GDVAWMLTSTLLVLMMALPGLALFYGGMVRSKNMLSISMQVFVTFSLISVLWAIYGYS
                     FAFTAGNAFIGGTDRLFLKGMIDAAGTLSNAATFSKGVYIPEYLYAAFQMTFAALTPC
                     LIVGAFAERMKFSAVLVFCVLWFTFSYLPIAHMVWFWAGPDAYTTKEAADAATAGAGF
                     LFQKGALDFAGGTVVHINAGIAGLVGCLLVGKRKGYGNMSLAPHNVPFTMIGAALLWV
                     GWFGFNVGSNLEANAFAAQVFINTFLATAAAVLAWAFGEWLVRGKPTMLGAASGAVAG
                     LVAITPACGWVGPMGSIVIGLIGGFVCMMAVLKLKAALGYDDSLDVFGVHCVGGILGA
                     ILTGVFNDTALGGTGIIDYVTGTWAYAGMGTQVMAQVWGVVVTLLWSGIVAFICFKIV
                     DITIGLRVADDVETEGLDTAEHGERSYHF"
     gene            complement(2287..2625)
                     /gene="glnK"
                     /locus_tag="PKZ52_13165"
     CDS             complement(2287..2625)
                     /gene="glnK"
                     /locus_tag="PKZ52_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNG60768.1"
                     /translation="MKLITAVIKPFKLDAVREALSDIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKTKLEVAVADGQVDTVVEAISKAAHSGKIGDGKIFVSSLEQVIRI
                     RTGETGEEAL"
     gene            complement(2698..3609)
                     /locus_tag="PKZ52_13170"
     CDS             complement(2698..3609)
                     /locus_tag="PKZ52_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNG60769.1"
                     /translation="MLVRISLLSPHFYVEFTASGQMVSVFGRVGLLPAKEFLHQAHNN
                     LKEAWHLRISFSDDFAITLEETSMKFTKSLLATAVAALASASLFAPVANAEVSGSVGV
                     ANMYYWRGLDLGTGDPQVSGDLKFSESGFYAGIWGGSGDSVGGTEYDLYIGYGNTIGD
                     IFKYDFSLWTYSYPSAPETSTSTSYKVVGSNVVAEDVVTPLDPFNTPGELSEAVLTLG
                     VGPVSLTYMDNIAGGTGYWYSTISATFDKFTILYGLHETDLAHVDLSYAYNDRLSFKI
                     GKVVDDVDGTFDDDVKFIASYSLPIGD"
BASE COUNT         1217 a         1092 c          884 g          879 t
ORIGIN      
        1 aaccagcgac caattggtta aaagccaact gctctaccga ctgagctaac gacccaaaag
       61 aggcgcacat cttaatgatt tataaactaa attcaaggtt tttttagtcg catagcaaaa
      121 gaatttagct tgctttgggc gcccagcaaa tttgacctaa gcctattctg cctccgaaaa
      181 caccagacga aaaaaaacgc ccctcagggc gtcttaggct aaagcaattt tacaccgcct
      241 caacccccgt ttcaccggtg cgaatgcgca caatctgctc taggggttgg ataaaaattt
      301 ttccatcacc gattttgccg gtttgtgctg ccttagtaat ggcctccaca accttatcca
      361 ccatatcggc ggagacagcc agctcaagct tcaccttcgg taaaaaatcc actacatatt
      421 ccgccccacg gtacaactcc gtgtggccct tttggcggcc aaagcccttt acctcagtaa
      481 ccgtcatacc ctgtacaccc acctccgaca aggcgatacg gacgtcatct agtttaaatg
      541 gcttaatgac cgcagtaatt agtttcatat caggaccctt aaatagcaaa cagacaaaca
      601 attcagataa acaaaacaag catagtgctg gcttagttta ggcaaggttt aagcaagttg
      661 cgcaacctag gccacaaaac ctgcaatgta cagcaagccg tcttaaaccc caaacaaaaa
      721 agcccctggg gtttccccca ggggcaaagt cagcctacgg cgacagatca agacatgaac
      781 cgaaccaacc tagaaatgat agctgcgctc accgtgctca gcggtatcca agccttcagt
      841 ttcaacatca tccgcaaccc gcagaccgat ggtgatatca acaattttga agcagataaa
      901 ggcgactatg ccggaccaca agagcgtgac caccacaccc catacttgcg ccatcacttg
      961 cgtgcccatg ccagcataag cccaggtacc ggtaacataa tcgataattc ctgtaccgcc
     1021 taaagccgtg tcattgaata caccagtgag gatggcgccg agaataccgc ccacacagtg
     1081 cacaccgaaa acatccaagg agtcgtcgta acccagagcg gctttcaact tcagaacagc
     1141 catcatacaa acgaagccac caatcaggcc gataacgatg ctccccatag gccccaccca
     1201 accacaggcg ggggtaatgg cgaccaggcc agcaacggcg ccagaggcgg cacccagcat
     1261 agtgggtttg ccacgcacca gccactcacc aaaggcccaa gccaatacag ccgcagcggt
     1321 cgctaaaaag gtgttgatga agacttgcgc ggcaaacgcg ttagcttcca ggttagagcc
     1381 tacgttaaag ccgaaccagc ccacccagag taaggcggcg ccaatcatgg tgaaaggaac
     1441 attgtggggc gctaacgaca tgttgccata acccttgcgc ttgccgacta acaaacaacc
     1501 tactaggccg gcaatacccg cattgatgtg caccacggtg ccgcctgcaa aatccaaggc
     1561 gcctttttgg aacaggaagc ctgctccagc agtggcggca tcggcagctt ctttcgtggt
     1621 gtaagcgtct ggaccagccc agaaccacac catgtgggcg ataggcaggt aagagaaggt
     1681 aaaccacagc acacagaaca ccaaaactgc agagaacttc atgcgctcag caaatgcgcc
     1741 aacgattaaa cagggagtta acgcggcaaa cgtcatttgg aacgcggcgt ataggtattc
     1801 cgggatataa acacctttac taaacgtcgc ggcattggaa agcgtaccgg cggcatcgat
     1861 catgcctttt aaaaataacc gatccgtgcc gccgataaat gcattgcctg cggtaaacgc
     1921 aaatgaataa ccgtaaatgg cccaaagcac cgagattaga ctgaaggtaa caaatacctg
     1981 catggagata gacagcatgt ttttagaacg caccataccg ccgtaaaaca gcgccaaacc
     2041 cggcaaggcc atcataagca ctaaaagggt actggtaagc atccaggcca catcaccttt
     2101 ttgtggaaca ggtggtgcca ccgcagcttc ttcggcgatt gccaaacccg gcaccaataa
     2161 agctgccgcc agcggccaca acactgcagc ccctttcagc actgagctta tagaaatatt
     2221 tttattcaca ggaattctcc taaacgaaag acctcgggta tttcccaggc cagacaagta
     2281 acaaggttaa agagcttctt cgccagtctc accggtgcgg attcgaatca cctgctccaa
     2341 agaagacaca aaaatttttc catcgccgat tttccctgaa tgcgcagcct tgctaattgc
     2401 ttcaactacg gtatcgactt gtccatcagc taccgcaact tctaatttag ttttcggtaa
     2461 aaaatccact acatattccg cccctcggta gagttcggta tggccttttt gacgaccaaa
     2521 accttttact tcagttacgg tgataccctg cacacctata tcggataagg cttctcgcac
     2581 ggcatccaac ttaaaaggtt tgatcacggc agtaattaat ttcatcggac tgctcctaaa
     2641 taaaatgcag agtacctgca gttggaaaaa tgcggccttt gcaggccgca ggattaatta
     2701 atcgccaatt ggcaagctgt aggaggcgat aaatttcacg tcgtcgtcaa aagtgccatc
     2761 aacatcatca accaccttac cgattttaaa gctgaggcga tcgttgtagg cgtaagataa
     2821 atccacgtgt gcaagatcgg tttcatgcaa accatagaga atggtaaatt tatcgaaggt
     2881 tgctgaaatt gtgctgtacc agtagccagt tccacctgca atgttgtcca tgtaagttaa
     2941 agaaacagga ccgactccca atgtcagcac cgcttcgctc agctcaccgg gtgtgttaaa
     3001 tgggtctaaa ggagttacca catcttctgc tactacattg cttccaacaa ccttgtaaga
     3061 agttgaggtt gaggtttcag gcgcgctggg gtagctgtag gtccatagac taaaatcata
     3121 tttaaagatg tcacctatgg tattaccata tccgatgtag aggtcgtact cggtaccacc
     3181 gacagaatcc cccgaaccgc cccaaatacc cgcatagaat cctgactctg aaaatttaag
     3241 gtcaccggaa acctgcggat cacctgttcc caaatccaaa ccacgccagt agtacatatt
     3301 cgcaacgccc accgaaccgc tcacctccgc attggctaca ggggcgaata aacttgcaga
     3361 tgccaaggcc gcaacagcgg ttgccaataa gcttttagta aatttcatgg atgtctcctc
     3421 caaagtgata gcaaaatcat cagaaaaact gattctcaaa tgccaagctt cttttagatt
     3481 gttgtgtgcc tggtgtaaaa actctttagc aggaagcaag ccaacacgtc caaacacaga
     3541 aaccatctgg cctgaagcag taaactcgac atagaaatgg ggtgacagga gggatatacg
     3601 caccaacata atgcatttgc acgaatttaa cgcaatgtgg cgcaccgata aatttcgtta
     3661 tggcgtatgg gcgttttaag tgctcgtcta attggaggaa cccgaagagt ttttgcggtt
     3721 acaactgggc agagcgggcc ctaattggtg cacacgaagg aaaaggttct acctatgcat
     3781 agggatgaag acttcccgca ctcaccaagc tggatctcag atcgacaaat atccccacgg
     3841 agattgattg gttaagcgct taatcggcca taggaaaagc catgaaggcc tgattaattg
     3901 gctatattca gcccaaataa gcagcaagcc ccctgaacaa acatttaggc ttcgcattat
     3961 tttctaatac ccatcttagg ttaatgaggc atccaaccat taattacaca aataaaaaag
     4021 gtgggagagt caactccgaa atgcatcaca gcccccggct agaaaaagaa gc
//
LOCUS       DAOQGS010000378         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12131_length_4052_cov_4.629972, whole genome shotgun sequence.
ACCESSION   DAOQGS010000378 DAOQGS010000000
VERSION     DAOQGS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12131_length_4052_cov_4.629972"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="PKZ52_13175"
     CDS             <1..1515
                     /locus_tag="PKZ52_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60770.1"
                     /translation="GAVAQVALSGTISYDRVGHLPNGGLNYSAISAMPARGVVVQLMG
                     SQNEVLASTLSDSQGNYQFAVKANTQVKVRVLAQLKNTNPTWAVDVADNTNSNAGYVL
                     EGSLVSSGSNSTQVRNLRAGSGWANGSYSQTRAAAPFAILDSIYDGLNLVASADPSLN
                     LPELHVRWSENNVSATGNYALGFIGSSMYSSYDNTIYILGKENNDTDEYDRGVIQHEL
                     GHFIEDKLSRSESLGGAHNLDSRVDMRVAFGEGWGNAFSGMAGGDSVYRDSMGATQSQ
                     TFKFDVEDNQGFAKGWFNERSIHSVLYDIFDSNNDGVDTVSLGFKPILAALHSSDYIN
                     FPGLTSIYPSIELIKRNNPQAESAITALMNNQAIYGTGMYGAGETNDSGYSTMLPIYE
                     QLSPGVSKEVCSNNHWQETNGADVRRYLLVTIPSDSQYTISVKGASTTDPDLRLWRNG
                     KFALQSTLDKAGVETVSKKLSQGVYTLEVYDSANADDDPNTGGKMCFSVSLTQG"
     gene            1520..2080
                     /locus_tag="PKZ52_13180"
     CDS             1520..2080
                     /locus_tag="PKZ52_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60771.1"
                     /translation="MKLTSLLIIAASQIAFNAQAGVGEFNFATLSKPSVFNPAAIHAH
                     GCARKPGAEIAISGREFFNLQPGQVDKLAVDLTGTPGQKITLHISAENKGLMVQGPEE
                     INLEVGSDQLASFNLPVKALLDGRWVINLIVHSETADGEVAGRALALVVQVGNMQVAG
                     GGQQKATQSDAQGAPVIVLPAQEEIL"
     gene            complement(2424..>4052)
                     /locus_tag="PKZ52_13185"
     CDS             complement(2424..>4052)
                     /locus_tag="PKZ52_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNG60772.1"
                     /translation="ISSWQEYIDNLGNLSQAIVSSLKKKARQTLLFDTMGTELSGAKL
                     LTAAITFAKRIKQQPEPAVGVLLPTGAAGVICNIAALFSGKTLVNLNYTASQEALLSA
                     LEQAGIRTVYTAERFLTKLAAKGMPLETMLSGVQLIKLEEFAKTITPREKLLTLFWVH
                     LLPSWLLQKIYTTPVAPEACAVVLFSSGSEGAPKGVQISHKNLLANVKQITDVLNMED
                     NDVVMANLPLFHAFGLTVTQFLPLVQGLPMVCHADPTDVLGSAKAIATYRATILFGTS
                     TFLRLYVRNTKIQPLMLNSLRFVVAGAEKLHAEVRSGFSQKFNIAIYEGYGATEVAPV
                     ASVNLPDKLELESWQTQLGQRAGSVGMPLPGTSVKIVDPQTFIELPTGEAGMILIGGP
                     QVMVGYLNNPEKTASAIKEIDGMRWYVTGDKGYIDEDGFIFIVDRYSRFAKIGGEMIS
                     LSQVETAIKEQINNPEIDVVVINLPDDKKGEKLIALHEAPLDTDNLRQQLQAAGLNPL
                     AIPGQWCLVAQLPKLGSGKTDFSAAKKLAEEAAR"
BASE COUNT         1011 a          936 c         1043 g         1062 t
ORIGIN      
        1 ggtgctgtgg cacaagtggc cttaagcggt actattagct acgatcgcgt gggccatttg
       61 cccaatggtg gccttaatta cagcgcaatt agcgccatgc ctgcacgggg tgtggtggtg
      121 caattaatgg gcagccaaaa tgaggtgctg gccagcacac taagcgatag ccagggcaat
      181 tatcaatttg cggtaaaagc taatactcaa gttaaagtac gcgtattagc gcaattaaaa
      241 aatacaaacc ccacctgggc ggtggatgtt gccgataata ctaatagcaa tgcaggttat
      301 gtattagaag gcagtttagt cagcagcggc agtaacagta cgcaagtgcg taatttgcgc
      361 gcgggcagcg gttgggctaa tggcagttac agccaaaccc gcgccgctgc accctttgcc
      421 attttagatt cgatttacga tggcttaaat ctggtcgcaa gcgccgaccc aagcttaaat
      481 ctgcctgaat tgcacgtgcg ttggagcgaa aataatgttt cggcaacggg taattatgcg
      541 ctgggtttta tcggtagctc catgtattcc agttacgaca ataccattta tattctcggt
      601 aaagagaata acgacaccga cgaatatgat cgcggtgtga ttcaacatga attaggccat
      661 tttattgaag ataaactgtc ccgcagtgaa agcctaggtg gcgcccataa tttggatagc
      721 cgcgtggata tgcgcgtagc gtttggtgaa ggttggggca atgcgttttc cggtatggcg
      781 gggggcgaca gtgtataccg cgattccatg ggggcaaccc aaagccaaac ctttaaattt
      841 gatgtggagg acaaccaggg ttttgctaag ggttggttta atgaacgctc cattcacagt
      901 gtgttgtacg atatttttga tagcaacaat gatggcgtgg atacagtttc cttaggcttt
      961 aaacccattt tggcggcgct gcacagcagc gattatataa actttccagg tttaacctct
     1021 atttacccct cgattgagtt gattaaacgc aacaacccgc aagcggagag tgcaattacg
     1081 gcattaatga ataatcaggc tatttatggt actggcatgt acggcgcagg ggaaactaac
     1141 gattctggct atagcactat gctgcctatt tacgaacaat tatcccctgg tgttagcaaa
     1201 gaagtgtgca gtaataatca ttggcaggaa accaatgggg ccgatgtgcg ccgctattta
     1261 ttggtgacta ttcccagcga tagccaatac accatcagcg taaaaggtgc cagtactacc
     1321 gacccggatt tacgcctctg gcgcaatggc aaatttgctt tgcaatccac tttagataaa
     1381 gccggtgttg aaaccgtgag caaaaaactc agccaaggcg tttataccct ggaagtgtac
     1441 gattctgcca atgccgatga tgacccgaat accggcggca aaatgtgttt ttctgtaagt
     1501 ttaacccagg gataattgta tgaaattaac cagcctgctt attattgccg ctagccaaat
     1561 tgccttcaac gcccaagccg gggtagggga atttaatttt gccactttaa gtaaacccag
     1621 tgtttttaac ccggcggcaa tccatgctca tggttgtgcg cgcaaacccg gcgcggaaat
     1681 tgccatcagc ggccgggaat tttttaatct gcaacctggc caggtggata agcttgcggt
     1741 ggatctcact ggcacgccag ggcaaaaaat taccctgcat atcagcgccg aaaataaagg
     1801 cctgatggtg caagggccag aggaaattaa tttagaagtt ggctctgatc agctggcgag
     1861 ttttaatttg ccagtaaaag cgctactgga tggccgctgg gtaattaatt taattgtgca
     1921 cagcgaaacg gccgatggtg aagtggcggg cagagcgttg gcgctggtgg tgcaagtggg
     1981 caatatgcaa gtggccggcg gtggccaaca aaaagcgacg caaagtgatg ctcagggcgc
     2041 tccggtaatt gtgttgcccg ctcaggaaga aattttgtag tggtggccgg tatttgtggc
     2101 agtggttagc gctgccagcc aatagggcaa actcaggagt aaaaaatctg cggcaggttg
     2161 gcaacaatcg ggcgagtgct tttttcactg taagtgctca gatcttgccc gcagcaacga
     2221 atacaggtta ttgccgccac accgcgcgcc ttattaatct cgatgctatt aatctcggtg
     2281 ctcttaatct cgttcccgtt tctcagcgct ttgcgtggga acgcctattc tcgggcaaat
     2341 atcttctctc gattcgtcac ccggtatgcg ttcccacgca gagcgtggga acgaggtaac
     2401 aagatattca gcggatttcg ctatcagcgt gcagcctctt cggccagttt tttcgccgca
     2461 ctaaaatccg ttttacccga gcctaattta ggcagttgcg ccaccaaaca ccattggccg
     2521 ggaatggcta ggggattaag ccctgccgcc tgtaattgtt gccgcagatt atcggtatcc
     2581 agtggtgcct cgtgcagggc aattaatttt tcgccttttt tatcatcggg caaattaatt
     2641 accaccacgt ctatttcagg gttgttgatc tgctctttga tggcggtttc cacctggctg
     2701 aggctaatca tctcgccgcc aattttggca aagcgggaat aacgatcgac aataaatata
     2761 aaaccgtcct cgtctatgta gcctttatca ccggtcacat accagcgcat gccgtcgatt
     2821 tctttaatgg cactggcggt tttttctgga ttatttaaat aacccaccat cacctgaggg
     2881 ccgccaatta atatcatgcc tgcctcgccg gtaggcagtt caataaaggt ttgcgggtcg
     2941 acaattttca cactggtgcc gggcagcggc atacccacag agcctgcccg ctgccccaat
     3001 tgtgtctgcc aactttccag ctctaattta tcgggcagat taacgcttgc caccggggct
     3061 acttcggtgg cgccataacc ttcataaatg gcaatattaa atttctggct aaagccgctg
     3121 cgcacttcag cgtggagttt ttccgccccg gctactacaa aacgcaagct gtttaacatc
     3181 aagggttgta ttttggtatt gcgtacatac aggcgtaaaa acgtactggt gccaaataaa
     3241 atagtggctc tataagtggc gatggctttg gcgctgccta atacgtctgt tgggtccgcg
     3301 tggcacacca tgggcaagcc ctgcaccagg ggtaaaaatt gtgtcacggt taaaccaaag
     3361 gcgtgaaaca gcggtaaatt ggccatgact acatcgttat cttccatgtt taacacgtcg
     3421 gtaatttgtt tgacgttggc taataaattt ttatggctaa tttgtacgcc tttgggtgcg
     3481 ccttcactgc cgctggaaaa taacaccact gcgcaggcct ctggggccac aggagtggta
     3541 taaatttttt gcagcagcca ggagggtaag aggtgcaccc aaaaaagtgt gagcagtttt
     3601 tcgcgcgggg taatggtttt ggcaaattcc tctaatttaa tgagttgtac gccgctgagc
     3661 atggtttcaa ggggcatacc ctttgcggcg agcttcgtta aaaagcgctc agctgtgtac
     3721 acggtgcgaa taccggcttg ctccagcgcg gataacagcg cctcttggct tgcggtgtaa
     3781 tttaaattca ccagggtttt accgctaaat aaagcggcta tattgcaaat cacaccagcg
     3841 gcccccgtgg gtaataaaac acccaccgca ggctcaggtt gttgctttat gcgtttagca
     3901 aaggtgatgg cggcggtgag taatttagcg ccgcttaact ctgtgcccat ggtgtcaaac
     3961 aaaagggttt gcctggcttt ctttttgagg ctgctgacaa tggcttggct taaattgcct
     4021 aaattgtcga tatattcctg ccaggagcta at
//
LOCUS       DAOQGS010000379         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12270_length_4021_cov_4.308623, whole genome shotgun sequence.
ACCESSION   DAOQGS010000379 DAOQGS010000000
VERSION     DAOQGS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12270_length_4021_cov_4.308623"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /gene="parE"
                     /locus_tag="PKZ52_13190"
     CDS             <1..1728
                     /gene="parE"
                     /locus_tag="PKZ52_13190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNG60773.1"
                     /translation="IDVILHKDGSVSVTDDGRGMPVDIHPEQGKPGVEVILSTLHAGG
                     KFSNKNYQFSGGLHGVGISVVNALSKILEVTIKRDGQVHRIGFRNGDKSKDLHVVDTC
                     PKRQTGTAVRFLPDPQYFDTAKFSVSRMRHVLRAKAVLCPGLRVSFLDEATGDKDDWY
                     YEDGLKDYLAASTQGFEVLPPNPFVGHFASEHAAVDWAVQWLPEGGELVQESYVNLIP
                     TAQGGTHVNGLRTGLLDAMRDYCEMRNLIPRGIKLAPEDIWNNISFVLSSKLADPQFS
                     GQTKERLSSREAAAFVSGVAKDAFSLWLNQHTDQSAALAEFCLSNAQKRVRSSKKVER
                     KKITQGPALPGKLADCSSADPARGELFLVEGDSAGGSAKQARDREYQAIMPLRGKILN
                     TWEVESAEVLASQEVHDISVAMGIDPGSDDLSGLRYHKICILADADSDGLHIATLLCA
                     LFVRHFPALVAAGHVFVAMPPLFRVDVGLDVYYALDESEKLGILERIAAEKKKGKVNV
                     QRFKGLGEMNPLQLRETTMSPDTRRLVQLVIDDSLATNQIMDMLLAKKRAGDRKSWLE
                     NKGDLAQVI"
     gene            2042..2782
                     /locus_tag="PKZ52_13195"
     CDS             2042..2782
                     /locus_tag="PKZ52_13195"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNG60774.1"
                     /translation="MTSRRTLLQESTALAAGLALSSAAFAKDTKAKKTEQPPYADRLT
                     DATGKYAAEPLPYAYNALEPVIDARTVELHYQFHHKPAVANAKKAEEALAHARDTGDF
                     ALIKHYEKELAFSLSSHILHTIYWTNLSPTGGAPSPELAKAIDLHFGSFDKLKAQLLA
                     AANSVEGSGWGILGYLPSTQKLTLLQCENHQKLTTWGVIPLLVLDVWEHAYYLHYQNR
                     RSEYTSAIFSIINWENVSARFKAALQQA"
     gene            2786..3736
                     /locus_tag="PKZ52_13200"
     CDS             2786..3736
                     /locus_tag="PKZ52_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNG60775.1"
                     /translation="MIQELPMNEWIALVLSLPTQNATVRMRAWRSLKASGVAVLRDGV
                     YLLPNRASGRQLFASIQDDVQASGGTAFILQVADDTEANFPALFDRQEEYALLLEELS
                     KLQATFSADSLASAIKQLRKLRKALAGIVAIDYFPTAALTQTEAALVAAEVQLNRLVS
                     PDEPHPMAGAIELLDPSNYAERYWATRRRPWVDRLASAWLIRRYIDATARFIWLESPE
                     DCPPHALGFDFDGATFSHVGAMVTFEVLLASFDLQQPALQRLGAVVHYLDTGGVQPPE
                     ASGLEQILWGLRNTLEDDDQLLSAACGVFDALLSAFIEKV"
     gene            3742..>4021
                     /locus_tag="PKZ52_13205"
     CDS             3742..>4021
                     /locus_tag="PKZ52_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNG60776.1"
                     /translation="MTTPAAATDQPPSSVSFWEAFRFWLKLGFISFGGPAGQISIMHQ
                     ELVERRRWISEKRFLHALNYCMVLPGPEAQQLATYIGWLMHRSWGGIVA"
BASE COUNT          907 a         1132 c         1102 g          880 t
ORIGIN      
        1 attgatgtga tcctgcataa agacggctcg gttagcgtca ccgatgatgg ccggggcatg
       61 ccggtggata ttcaccccga gcagggcaag ccaggagttg aggtaattct ctccaccctg
      121 cacgcgggcg gtaaattctc caacaaaaat tatcaattct ccggcggttt gcacggtgtg
      181 ggtatttctg tggtgaatgc cctgtccaaa atcctcgaag tcaccatcaa acgggacggc
      241 caggtgcacc gcatcggctt tagaaacggc gataaatcca aagacctgca tgtagtggat
      301 acctgcccga aacgccaaac cggcaccgcc gtgcgttttt tgccggaccc acaatacttt
      361 gataccgcta aattctccgt ctcccgcatg cgccatgtgc tgcgcgccaa ggccgtgctc
      421 tgccccggtt tgcgggtgag ttttttagat gaagccaccg gcgataaaga cgattggtac
      481 tacgaagacg gcttaaaaga ttatctcgcg gccagcaccc agggctttga agtgctgccc
      541 cccaacccat ttgtgggcca cttcgcctcc gaacacgccg ccgtggattg ggccgtgcaa
      601 tggctgcccg agggcggtga gctggtgcag gagagttacg ttaacctgat tcccaccgcc
      661 cagggcggca cccacgtcaa cggtctgcgc accgggctgt tagatgccat gcgcgactac
      721 tgcgaaatgc gcaatttaat cccccgcggc attaagcttg ccccggagga tatttggaat
      781 aacatcagtt ttgtcctctc cagcaaatta gcggaccctc aattctccgg ccaaaccaaa
      841 gagcgtttat cctcccgcga ggcggcggca tttgtttccg gtgtggccaa agacgccttt
      901 agtttatggc tcaaccaaca caccgaccaa agcgcggccc tggccgaatt ttgtttatcc
      961 aacgcgcaaa aacgtgtgcg ttcaagcaaa aaagtcgagc gaaaaaaaat cactcagggc
     1021 ccagctctac cgggcaaact tgccgactgc tccagcgccg atcctgcgcg gggtgaatta
     1081 tttttagtgg aaggagactc ggccggtggc tctgccaagc aggcgcggga tcgggaatac
     1141 caagccatca tgcccctgcg cggcaaaatc ctgaacacct gggaagtgga aagtgcagag
     1201 gttttagcct cccaagaagt acacgatatt tccgtggcca tgggtataga cccaggctca
     1261 gatgacctct ccggcctgcg ctaccacaag atttgtatcc tcgccgatgc ggactcggac
     1321 ggtttacaca tcgccactct tctatgcgcc ctgtttgtgc gccacttccc cgcattggtg
     1381 gcggcgggcc atgtgtttgt agccatgccg ccgctatttc gcgtcgatgt gggcctggat
     1441 gtctattacg ccttagatga aagcgaaaaa ctcggcattt tggagcgtat cgcggcggag
     1501 aagaaaaaag gcaaggtgaa tgtgcagcgc tttaaaggct tgggtgaaat gaacccgctg
     1561 caattgcgcg aaaccaccat gtcgccggac acccgccgcc tggtgcaatt agtgattgat
     1621 gacagccttg ccaccaatca aatcatggat atgttattgg cgaaaaaacg cgccggggat
     1681 cgtaaatcct ggttggaaaa taaaggcgat ttggcgcagg tgatttaatt tagccgctac
     1741 actcaggggg ttagcgcagt tcactaacac cctcctttct caactgcgcc gcttaggtgt
     1801 gaacaccagc ggccttcctg cccttgttta ccaagtacaa cacagccata gccggataac
     1861 accagggttg cgccaaggtg aacgatacgt tggcagcctt gtacattgcg cctgagagca
     1921 gtatcccagg cgcagggcag agcaaatcag taaatatact tacaaacttg tttatagctc
     1981 tacacaccca tatggacgag gtggtatatt cgccgctctc tcccgcgtgc aggctcttta
     2041 tatgaccagt cgacgaacgc tcctacaaga atcaacggca ttggccgccg ggcttgcact
     2101 ctccagtgcg gcgtttgcca aagacactaa ggctaagaaa acagaacaac ccccatacgc
     2161 cgaccgcttg accgatgcaa cgggcaagta tgctgccgag cccctgccct atgcctataa
     2221 cgccttggaa ccggtaattg acgcgcgcac agttgaactg cattaccaat tccaccataa
     2281 accggctgta gccaatgcca aaaaagcgga agaggccctg gcccatgccc gcgacacggg
     2341 tgactttgcc ctgatcaaac actatgaaaa agagctggcc tttagtctgt ccagccacat
     2401 attgcacacc atttattgga caaacctctc ccccacaggg ggtgccccct caccggaatt
     2461 agccaaagcc atcgacctgc actttgggtc gtttgataag ttaaaagccc agttactggc
     2521 ggctgccaac agtgttgaag gaagtggctg ggggattttg ggttacctgc cgagcacgca
     2581 aaaactcacc ctattgcagt gtgaaaacca tcaaaagctg accacctggg gtgttattcc
     2641 cctgctggta ctggatgtgt gggagcacgc ctattacctg cactaccaga accgccgcag
     2701 tgaatacacc agcgctattt ttagcattat taattgggag aacgtgtccg cgcggtttaa
     2761 ggcagcgctg cagcaggcct aggaaatgat tcaagagttg cccatgaacg agtggatcgc
     2821 gttggttttg tcgctgccca ctcaaaacgc caccgtgcgg atgcgcgcct ggcgctcact
     2881 gaaggcgtcc ggtgtcgccg tgctgcgcga tggcgtgtat ctgctgccca atcgggcaag
     2941 cggtaggcag ttgtttgcat cgattcaaga cgatgtgcag gcatctgggg gtactgcctt
     3001 tattttgcag gttgcagatg acaccgaggc caatttccct gcgctgtttg accgccagga
     3061 ggaatacgcc ctgctacttg aggaactctc caaactgcag gcgacattca gcgcggactc
     3121 cctagcgagc gccattaaac agttgcgtaa gttgcgcaaa gccttggcgg gtattgtggc
     3181 aattgattat ttccccactg cggcgctcac ccaaaccgaa gcggcattgg tggcggcgga
     3241 agtacagctt aatcgtttgg tgtcacccga tgagccgcac ccgatggcgg gcgctattga
     3301 gctacttgat cccagcaact atgcggagcg ttattgggcc acccgcaggc gcccctgggt
     3361 ggatcgcctc gccagtgctt ggctgatccg gcgctacatc gatgcaactg cgcggtttat
     3421 ctggctggag tcaccggagg attgcccgcc ccatgccctg gggtttgact ttgacggtgc
     3481 caccttcagc cacgtcgggg ccatggtcac ctttgaggtg ctgctggcca gttttgacct
     3541 gcaacaacca gcgctgcagc gcctgggcgc agtggtgcat tacctggata ccggtggtgt
     3601 gcaaccccct gaagccagtg gccttgaaca aatcctatgg ggcttgcgca atacccttga
     3661 ggatgacgat caactcttaa gcgcagcctg cggcgtattc gacgcgctgc tgagcgcatt
     3721 tatcgaaaag gtatgagcat catgacaaca cccgctgcgg caactgatca accaccctcc
     3781 agtgttagct tttgggaggc ctttcgtttc tggctcaaat tgggctttat cagttttggt
     3841 gggcctgcgg gccaaatatc gatcatgcat caggagctgg tggagcgtcg ccgctggatc
     3901 agcgaaaagc gcttcttaca cgcattgaat tactgcatgg tgctgccggg gccggaggcg
     3961 cagcagctgg ccacctatat cggatggctg atgcaccgca gctggggcgg catagtggcg
     4021 g
//
LOCUS       DAOQGS010000380         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12321_length_4013_cov_3.445680, whole genome shotgun sequence.
ACCESSION   DAOQGS010000380 DAOQGS010000000
VERSION     DAOQGS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12321_length_4013_cov_3.445680"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKZ52_13210"
     CDS             <1..386
                     /locus_tag="PKZ52_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60777.1"
                     /translation="PTPTGTVRHKLSLNTCDDCHSGETLTQFTHVKPTTFPAALSSFV
                     QGGVVVTDPGGEIGVSRKFDDLTRRGQILEDLAVKSCRSLIPRINDPLVLNPRIQPTF
                     KLIKPQVDPTPVSFSRQQQVHTFVH"
     gene            1129..2106
                     /locus_tag="PKZ52_13215"
     CDS             1129..2106
                     /locus_tag="PKZ52_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG60778.1"
                     /translation="MTATPRKTLFNRFLIGLTLALSAFSCQIATRAFAVDEVMTPITT
                     PAQPTAIELGTGPLADATAQESWHRQFGSVFARNVTVATLTPFLPSKRKASGAAVIVA
                     PGGGFRTLSMSNEGWDVAKALADKGVAAFVLKYRLKQTPAEMAAFEKAMAEMFSGASR
                     ANRPLAEQDPAVEFAVQIADAQAAFALVRARAKEWRVDPDRIGMIGFSAGAMLTISTT
                     LYSKEAKPAFIGDIYGPLTKVDVPADAPPLFAALAADDPFFANAGYGLIDSWRAAKRP
                     VEFHLFEQGGHGFGMYPKPTTSTGWFDSFVRWLDMHGYLKPKTGKETGK"
     gene            complement(2297..3169)
                     /gene="sucD"
                     /locus_tag="PKZ52_13220"
     CDS             complement(2297..3169)
                     /gene="sucD"
                     /locus_tag="PKZ52_13220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNG60779.1"
                     /translation="MSVLINKNTKVLCQGFTGSQGTLHSEQAIAYGTNMVGGVTPGKG
                     GQKHLGLPVFDTMKDAVADTGADATVIYVPAPFCKDSILEAAYSGVKLIVCITEGIPT
                     LDMLQCKVRCDELGVRLIGPNCPGVITPGECKIGIMPGHIHLPGRVGIVSRSGTLTYE
                     AVKQTTDFGFGQSTCVGIGGDPIPGSNFIDILTLFQNDPQTEAIVMIGEIGGTAEEEA
                     AAFIKANVKKPVVSYIAGVTAPPGKRMGHAGAIISGGKGTAAEKFAALHDAGVATVKS
                     LADIGKTLKEVTGW"
     gene            complement(3169..>4013)
                     /gene="sucC"
                     /locus_tag="PKZ52_13225"
     CDS             complement(3169..>4013)
                     /gene="sucC"
                     /locus_tag="PKZ52_13225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNG60780.1"
                     /translation="YLGAVVDRSTRRIVFMASTEGGVEIEKVAEETPEKILKAVIDPL
                     TGAQPYQARELGFKLGLNSEQIKQFTKIFIGLAKMFQDFDFALLEINPLVITKQGNLH
                     CLDGKINIDGNALFRQPKIKAMHDPSQEDAREAHASAWELNYVALEGNIGCMVNGAGL
                     AMGTMDIVKLHGGQPANFLDVGGGATKERVVEAFKIILSDDNVKAVLINIFGGIVRCD
                     MIAEGVIGAVKEVGVKVPVVVRLEGNNAELGAKVLADSGLNIIAATSLTDAAQQVVKA
                     AEGK"
BASE COUNT         1010 a         1106 c          940 g          957 t
ORIGIN      
        1 caccaacccc aaccggcacc gttcgccata agttatcgtt gaacacctgc gacgactgcc
       61 actccgggga aaccctcacc caatttaccc atgtaaaacc caccacattt ccagcggcat
      121 tatccagttt tgtgcagggc ggcgtagtgg tgaccgaccc gggcggcgag atcggcgtca
      181 gccgaaaatt cgatgattta acccgtcgcg ggcaaattct tgaggacttg gcggtaaaat
      241 cctgccgcag tttaattccg agaattaatg acccattagt actaaatccg cgcattcaac
      301 ccacgtttaa actcattaaa ccgcaggtgg atccaacacc cgtgagtttc agccgccagc
      361 agcaagtcca tacgttcgtt cactaaccgt atagccaccc taaggttttg aggggatatt
      421 cacacggcgc gcaaatttat tttgcgcgct tttttatttt ggctctcgca aaagctattt
      481 aacgcctgcc acacagcgcc aaaagcgcaa cactcgccaa tcgcattgag ttcaataggt
      541 ttaattaaaa tcaactatga attagggaat aaagtgtaac aaacacgaac ccagcaactt
      601 gtagcgccat acgcgtcctg gctacagctt caaatatccc cgcctgcata cttattcaga
      661 gtttctgaaa attgattgcg cacacatgca cattaatttt ataaaacaca tcgcgccacc
      721 aagctaaaaa taaacacacc actcctgctg cgaaaataaa cttctgcgtt tacaaaattt
      781 gcacatatgc aaacataatt aacgcctcac agaggtagac tgcccaagaa gattgaactc
      841 ccacgcaggc tgcaaaaaaa cggcatttgg cctgggcatg gcatctattg caatattcag
      901 gttttgaatg gacatttcac gcccaattaa gcggcacgcg cattttgtta gcaacaaaaa
      961 gactacaagg ctaataattg ttaaaaatgc aaagttcaac cgctacaacc actgtccact
     1021 taattacgcc gcactgtatt atcatatgac cagtgtcgat tagttcaccg caaaagagcc
     1081 ctacagataa ctggcacgag tagataacaa cagaccatag gaaattaaat gaccgccacc
     1141 cctcgcaaaa cccttttcaa ccgcttttta atcgggctca cccttgctct ctccgccttc
     1201 agctgccaaa tagccacgcg cgcattcgca gttgatgagg tgatgactcc gataaccact
     1261 ccagcccagc ccaccgcaat tgaactggga accggccccc tagcggacgc caccgcccag
     1321 gagtcctggc accgccaatt tggcagcgtg tttgcgcgca atgtaactgt agccacccta
     1381 acaccctttt taccttcaaa acgcaaggct tcaggagcgg cggttatagt cgcccctgga
     1441 ggcggttttc gcactctgtc tatgagcaat gagggctggg acgtagcaaa agcccttgcc
     1501 gataaaggcg tggcggcttt tgttctcaaa tatcgcctaa aacaaacccc cgccgaaatg
     1561 gccgccttcg aaaaagccat ggccgagatg ttctccggcg ccagccgcgc caatcggcca
     1621 ctcgccgagc aagacccagc ggtagaattt gccgtgcaaa tcgccgacgc ccaggcagcc
     1681 tttgccctgg tgcgcgcgcg ggctaaagaa tggcgcgttg acccagatcg catcggtatg
     1741 atcggctttt cggcgggtgc catgctcacc atctccacca ccctctacag caaagaagcc
     1801 aaaccggcgt ttatcggcga tatctatggc cccttaacca aagtggacgt acccgccgac
     1861 gcgcccccac tgtttgcggc cctagcggca gacgacccat ttttcgccaa cgcaggctat
     1921 ggcctcatcg acagctggcg cgcggccaaa cgcccggtgg agtttcattt attcgaacaa
     1981 ggcggccacg gcttcggcat gtaccccaaa cccaccacta gcacaggctg gttcgacagt
     2041 tttgtgcgtt ggttggatat gcatggatat ttgaagccta aaacaggaaa agaaacaggg
     2101 aaataactgc tcaatattcc ctctccagtt aaacctttct gcagtggtct aaggctgagg
     2161 atttaattct tttttggggt taaaaatatc ggtttggcta taactcctaa tatttgatct
     2221 agctgtcact gacgaataaa acccataaaa aaaccgccga ggctgcccag ggcggttttt
     2281 tattaccgag caataattac cagccagtaa cttctttcaa ggttttacct atatcggcca
     2341 agcttttaac tgtggcaaca ccagcatcgt gtaaagccgc gaatttctcc gccgctgtac
     2401 ccttgccacc ggaaataatg gcacctgcgt ggcccatacg tttgccggga ggcgcagtta
     2461 cacctgcgat gtaggacaca acgggctttt taacattggc tttgataaag gctgccgctt
     2521 cttcctcggc ggtaccgcca atttcaccga tcatgacaat cgcttccgtt tgcgggtcgt
     2581 tttggaacag ggtgagaatg tcaatgaagt tggagcctgg gattgggtca ccgcctatac
     2641 ccacgcaggt ggattggcca aaaccgaaat cagtagtttg cttcacggct tcataggtaa
     2701 gggtaccgga gcgagaaaca atacccacgc ggccaggtaa atggatatga ccgggcatga
     2761 tgccgatttt acattcgccg ggagtgatta cacctgggca gttagggccg atcaaacgca
     2821 cgcccaattc gtcacaacgc actttacact gcagcatatc gagggttgga ataccttctg
     2881 tgatacacac gatcagcttc acgccgctgt aggctgcctc caaaatggag tctttacaga
     2941 aaggcgcagg tacgtaaatt acggtggcat cggcaccggt atccgccacg gcatctttca
     3001 tggtgtcaaa tacaggcaga cctaagtgtt tttggccgcc tttaccgggg gttacaccgc
     3061 ctaccatgtt ggtaccgtag gcgatggctt gttctgagtg gagtgtacct tgtgaaccgg
     3121 taaaaccctg gcagagcacc ttggtatttt tgttgattaa tacgctcatt atttaccctc
     3181 cgcggctttt accacttgct gcgcagcatc tgtcaggctg gtggcagcga tgatgttcag
     3241 gccgctgtcg gccagtactt tagcgccgag ctcggcgttg ttgccttcaa ggcgcacaac
     3301 cacaggcact tttacgccca cttcttttac cgcgccaatt acaccttcag cgatcatgtc
     3361 gcagcgcacg ataccgccga agatgttgat caagacggcc ttaacgttat cgtcggagag
     3421 aataatttta aacgcctcaa ccacgcgctc tttagtggcg ccgccaccta cgtcgaggaa
     3481 gttagctggc tggccgccgt gtaatttcac tatgtccatg gtgcccatgg cgaggcctgc
     3541 gccgttcacc atacaaccga tgttgccctc gagggcaacg tagtttaatt cccaagcgga
     3601 ggcgtgggct tcacgggcat cttcctgtga aggatcgtgc atggccttaa ttttgggttg
     3661 gcggaacagg gcgttgccgt cgatattaat tttgccgtcc aggcagtgca gattgccttg
     3721 tttggtgatc actagcgggt taatctccag cagggcaaag tcaaagtctt ggaacatttt
     3781 tgccaagccg ataaaaatct tggtgaactg cttaatttgt tcgctgttta ggcccaattt
     3841 aaagcccagt tcgcgggctt gatagggctg cgcaccagtt aaaggatcga taacggcttt
     3901 aagaattttt tctggcgttt cttcagctac tttttcgatt tccacaccgc cttcggtaga
     3961 cgccatgaac acgatacggc gggtagaacg atccaccact gcgcctaaat aca
//
LOCUS       DAOQGS010000381         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12339_length_4009_cov_4.385432, whole genome shotgun sequence.
ACCESSION   DAOQGS010000381 DAOQGS010000000
VERSION     DAOQGS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12339_length_4009_cov_4.385432"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            899..1381
                     /locus_tag="PKZ52_13230"
     CDS             899..1381
                     /locus_tag="PKZ52_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60781.1"
                     /translation="MNYLVNSIILSGLCALSAAAMADTARSFERETVRTSAKGTTTVS
                     VDQDATANGWVRNKEITRPDGKVAERHTEVNRDPSTGVVTRSVDGTKFNGKTYSKDST
                     RTPTENGFIKETTQTTPNGKTREKLKVVERSPGERSVDVTRVNGQGKVFHRNTHTTRN
                     "
     gene            1391..1972
                     /locus_tag="PKZ52_13235"
     CDS             1391..1972
                     /locus_tag="PKZ52_13235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNG60782.1"
                     /translation="MAVLAFFNRKRQELALEQFLAATERRAYQSALLTTRREADALDI
                     VQDAMLQLVQHYREHGAEEWPKLFQRILQHKIVDWVRARHRQQGLYCQLDADENDSDP
                     WERILDPRDINPAAILMAQQDVQRVLNALEILPLRQRQCFLLRAWEGFDTEATAQALG
                     ISSGSVKTHYARALTKLNTALTCPAPQEPSYAH"
     gene            1962..2300
                     /locus_tag="PKZ52_13240"
     CDS             1962..2300
                     /locus_tag="PKZ52_13240"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HNG60783.1"
                     /translation="MPTDHHTDPSRQEADAAAQFSQALVKSLNQATEQLPRSTQAALA
                     ASRAQALGQKKTHWHWALAASLLLALGMLTWHAPPPDTDAMALEILQTDDQLLNEIDL
                     LESLAEVDET"
     gene            2297..2578
                     /locus_tag="PKZ52_13245"
     CDS             2297..2578
                     /locus_tag="PKZ52_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60784.1"
                     /translation="MSRLNLPRSPSLRLLHASLWALALMGAAGARAEATAPRPQAAQE
                     APLDSEFWELYDELADDSGRLPAPEEIPPPKESLLPTPKPDNKGAKEEL"
     gene            2575..3075
                     /locus_tag="PKZ52_13250"
     CDS             2575..3075
                     /locus_tag="PKZ52_13250"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNG60785.1"
                     /translation="MKFIILLLIMCSLGTQVWAADTSWSSLTAEQQSLLAPLKDKWPQ
                     LPQKRREKLLQRAQQYAALPEDQRAQVRERFARWQALKPEEKQQLRANFQRFQQLSPE
                     EQAEVKQRWAWFKQLPPSEQEALRSKWRSLSPEQRQAIRQKIRAMSPEQRQKARERLI
                     QRRLNQ"
     gene            3084..3956
                     /locus_tag="PKZ52_13255"
     CDS             3084..3956
                     /locus_tag="PKZ52_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60786.1"
                     /translation="MNFPASALPPLAALTLSLAAAPALAELDWQARANLSHSQQAYDE
                     QPSQAQTQLLLSSLWRDDNWDLTFSLPLISMDANYQIEPGRTPNLCARIQAAKPQKLA
                     RWLRLGRVNQKTIDRCLAQANTAEDETVSGLGDARLEANYYWQPSDNLELSLGSGVKA
                     ANAEPSKNLGSGRESLYLMGGLTYLWSSVSAGVSGEYSQHLGEGGPDEQDSNQSLSAH
                     LDWQWQPSLSLGAFTRWESASFQDEDDLVSSGVAFTYQPIKNLSLGLSIERFQPNSQG
                     LSRSLTGHIAYSAF"
BASE COUNT         1031 a         1252 c         1039 g          687 t
ORIGIN      
        1 cacaccattt gggaaaggcg ctcgccgcta aggcttttaa gccttcaaat atgtgtttat
       61 ctaaataatc cgaatgcata gcctaacgtt tacacctaac cattcacaca ggttgcagag
      121 aatttaacgc ccaccgatag ggcgtttagc aaccgacaag ctgcgtttaa cctataaaaa
      181 caggctaaaa ccacactcca gtctgcacag gcatcggtaa attcgctgcc gttttaaaat
      241 tatagcgcta cacctctatt aatcagccgt tgtaaaaaaa cagtctaaaa attctgttta
      301 aggatcaaaa acttaagggc agccaggatt gacggatacg ggcacgcgct aaaattagca
      361 gggattgtgt ttagccatac tccccttaaa aagggggagg ctgggaggca gtgttattaa
      421 gtaaagagtt ttgagatgaa tctttacccg ttggttggaa gagcacagcg aattccgaca
      481 tcccgcagaa tccctgccgt agctatagtt tggtctggcg gatggatgtc ggaagtcgct
      541 gcgctcttcc aaccgacgct atagcgggcc tttcccataa cttaatgact ttgggccggg
      601 ggctggcgct aggtactctg caaataggcc aaaatgcata actgattgac attactcggg
      661 caggctttaa ataacacagc gcccggccaa cacaacttca gtaataggca cctgcggcgc
      721 tcatcggtat cctggccgcc gcaaacaggg ttcgggccag caaaaaaatt gcccccacct
      781 gtcaaccaac ccgcagcccc cgcgttaata gaggtaactg gcaaaaaacc agcggcaaca
      841 ccgggccaca atcttaagtg gcccataatt aacctcatca acaggcagag gcatcagcat
      901 gaactattta gtcaacagca ttattttaag cgggctctgt gcactcagtg ccgcggctat
      961 ggcagatacc gcccgctcct ttgaacgcga aaccgtgcga accagcgcca aaggcaccac
     1021 caccgtctcg gttgaccagg acgccaccgc caatgggtgg gtgcgcaata aggaaatcac
     1081 ccgccccgat ggcaaggtcg ccgagcgcca cactgaggta aatcgcgatc cttcgaccgg
     1141 tgtagtaacc cgttcagtgg acggtacaaa atttaacggc aaaacctaca gcaaagattc
     1201 cacccgcacc ccgaccgaaa atggattcat taaagaaacc acccaaacaa cccccaacgg
     1261 taaaacccgc gaaaagctca aagtggtaga gcgcagcccg ggggagcgct cggtggatgt
     1321 cacgcgggta aacggccagg gtaaggtgtt ccaccgcaac acccacacca ctcgcaacta
     1381 attaaacccc atggccgtgt tggcattttt caatcgcaag cgccaggagc tggcgctgga
     1441 gcaatttctt gcggccactg agcggcgcgc ctatcaaagc gccctgctca ccacccgccg
     1501 ggaagcggac gccttggata tagtgcaaga cgccatgctg cagctggtgc agcactatcg
     1561 cgaacacggt gccgaagagt ggccgaagtt attccaacgg attttgcagc acaaaatcgt
     1621 cgattgggtg cgcgcccggc accgccaaca gggcctctat tgccaattag atgcggatga
     1681 aaacgactct gacccctggg agcgcatcct agacccccgc gacatcaacc cggcggcgat
     1741 acttatggcc cagcaggatg tacagcgggt gttaaacgcc ctggagatat tacccctgcg
     1801 ccagcggcaa tgttttttgc tgcgcgcctg ggagggtttt gatactgagg ccacggccca
     1861 ggccctgggg attagcagcg gcagtgttaa aacccactac gcccgcgccc ttacaaaact
     1921 caacaccgcc ttgacctgcc ctgccccaca ggagcccagc tatgcccact gaccatcaca
     1981 ctgatccatc ccgccaagag gcagacgccg cagcgcaatt cagccaagcc ctggtgaaga
     2041 gcctcaacca ggccaccgag caactgcctc gctccaccca ggcggccttg gcggcaagta
     2101 gggcgcaggc cttagggcag aaaaaaaccc actggcactg ggccttagcc gcatcgctgc
     2161 tgctggccct gggcatgctc acctggcatg ccccgccgcc agataccgac gccatggccc
     2221 tggaaatttt gcagactgat gaccagctgc tcaacgaaat cgacctgttg gaaagcctgg
     2281 ctgaggtgga cgagacatga gccgtctgaa tctaccccga agcccaagcc tcaggctgtt
     2341 gcacgctagc ctgtgggcgc tcgccctgat gggggccgca ggcgcgcggg cagaggccac
     2401 cgccccgcga ccgcaagccg cacaggaggc gccattggac agcgaatttt gggagctgta
     2461 cgacgagctg gccgatgaca gcggccgcct gcccgccccc gaagaaatac caccgcccaa
     2521 agaatcccta ttgcccacgc ccaagcccga caacaaaggc gcgaaggagg aactatgaaa
     2581 ttcatcatcc tgctactgat catgtgcagc ctaggcacac aggtgtgggc cgccgatacc
     2641 agctggtcgt cacttactgc cgagcagcag agcctgctgg cccccttaaa agacaaatgg
     2701 ccccaattgc cccagaagcg acgggagaaa ctgctgcaaa gggcccagca gtacgccgct
     2761 ctgccggaag atcagcgcgc ccaggtgcgc gagcgcttcg cccgctggca ggctcttaag
     2821 cccgaagaaa aacagcagct cagagccaac tttcaacgct ttcaacaact gagtccagag
     2881 gagcaagccg aagtaaaaca gcgctgggcc tggtttaaac aattgccccc tagcgagcag
     2941 gaagccctgc gcagcaaatg gcgcagccta agcccggagc agcgccaggc catacggcaa
     3001 aaaatccgcg ccatgagccc agagcagcgc caaaaagccc gcgaacggct gatccaacgt
     3061 cgtcttaacc aataaggtat gccatgaatt ttcccgcttc agctttgccg cccctagccg
     3121 ccttaaccct gtcactggcc gccgcacctg ccctggccga actggattgg caggcccgag
     3181 ccaacctgtc ccacagccaa caggcctacg acgaacaacc cagccaagca caaacccagc
     3241 tgttactgag cagcctttgg cgggacgaca attgggacct cacattcagc ctgccattga
     3301 tcagcatgga tgccaattac caaattgaac cgggtagaac ccccaaccta tgcgcccgca
     3361 tacaagccgc caaaccgcaa aaactggccc gctggctgcg cctaggacgc gtcaatcaaa
     3421 aaaccatcga tcgctgtttg gcccaggcca acacggcaga agacgagaca gtatccggcc
     3481 tgggcgatgc ccgcttggaa gccaactatt actggcaacc cagcgacaac ctggagctaa
     3541 gcctcggcag cggggtaaaa gccgccaacg ccgagccgtc taaaaacctc ggcagcggcc
     3601 gggaatccct ctacctaatg ggtggcctga cctatctgtg gtccagcgtc agcgcagggg
     3661 tcagcggcga atacagccaa cacctaggtg aaggtggccc agatgaacag gacagcaacc
     3721 aaagcctcag cgcgcaccta gactggcagt ggcaacccag cctcagcctg ggtgccttca
     3781 cccgctggga atcggcaagc ttccaggatg aggacgacct cgtcagcagc ggcgtggcct
     3841 ttacctacca acccatcaaa aacctcagcc tcggcctcag tattgagcgc ttccagccca
     3901 acagccaggg cctcagccgc agcctcaccg gtcatatcgc ctacagtgcg ttttaaccta
     3961 aaaaccgcag ttgaactcgt agcgagggtg cctaagacgc tgaatatgg
//
LOCUS       DAOQGS010000382         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12394_length_3999_cov_4.525101, whole genome shotgun sequence.
ACCESSION   DAOQGS010000382 DAOQGS010000000
VERSION     DAOQGS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12394_length_3999_cov_4.525101"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1566
                     /locus_tag="PKZ52_13260"
     CDS             271..1566
                     /locus_tag="PKZ52_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNG60787.1"
                     /translation="MIKKIDGKSLAKACLGAYKAAGLIFVSECVMHLSVTELYLIAMC
                     LIFSVPYLIWRLGRTDYWAPLVVVQIISGILLGPGVMGAWQPEYYKFVFTPDVIKCLN
                     GVAWWAVMMFVWIAGIELDLKKAWQYRRESGITAGLALGMPLLFGCVAGFGLLNYQGW
                     MGSKALTWQFVLGVGMACAVTALPILILLMEKLDILRAPIGQRILRYASLDDIAIWGV
                     LAVILMDWERVGRQVGFLVVFAAAAFLFRKLMCAIEEKDRWFAGIMWLALCGLGADWS
                     GLHFMVGAFLAGAVMDADWFNQEHMDLLRHHLLLTVMPVFFLSTGLRTNWDVGGMAVF
                     GVAAILLVASISGKLAGVHLAGKWLRWAPGEASLIGWLLQTKALIMIIFANVLLDKQI
                     ITNETFTALLLMAVGSTMLTTPVVAPKLKRLGALLQRET"
     gene            2072..2542
                     /locus_tag="PKZ52_13265"
     CDS             2072..2542
                     /locus_tag="PKZ52_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNG60788.1"
                     /translation="MHPIDIGINEPQRLQIAEGLSQLLADSYTLYLQTHNFHWNVVGP
                     HFRELHLMFQEQYTELAVAVDDIAERIRALGVAAPGTYQAFARLSKIKEVEGVPSASE
                     MVQILTTGHEQIVKTCRAVLKRSQAADDESTSSLASDRMRVHEKTAWMLRAVAG"
     gene            complement(2543..3466)
                     /locus_tag="PKZ52_13270"
     CDS             complement(2543..3466)
                     /locus_tag="PKZ52_13270"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNG60789.1"
                     /translation="MPTSTSNSSSRLLKLLLGQREKWLSGLGAFIAISTVALGTQWLL
                     PQAALVILPSMGASAVLLFALPHAPLSQPWPVVGGHLISGVIGVAVQQWLPGEFAAAL
                     AVGAAITAMYFLRCLHPPGGATALMAVIGGGAGWAFVWQVCLLNLVLILLAALVFNAP
                     FPWRRYPNAWHSKPTSPLPFSFAQEDFAAAITEIDTLVDVSPEDLQQLVRLACEHAQA
                     QKHLDAPIAAGCYYSNGALGDSWAVRLVLDINLDEPKAMRRKVIYKIVAGEGLYSIHL
                     CSLGEFTAWARQQVEQHNGHYVPHRTRSGKA"
     gene            3617..>3999
                     /locus_tag="PKZ52_13275"
     CDS             3617..>3999
                     /locus_tag="PKZ52_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60790.1"
                     /translation="MRRHLTWLLLFTLLAPRSWALELPSLLADWQGWVLEDEAEQFCP
                     KALEDDRKECVWPGELTLQASDKGADFKLAVDVFAPSWVDLPGGPGYWPQEVKNGSSW
                     IPVRASSERPQVYLLKGRHLLSGRF"
BASE COUNT          838 a          982 c         1185 g          994 t
ORIGIN      
        1 gcatgtagcg cagcgagaaa ttctgcgcta cttggcgctg ttcctgctgg ctcatcagca
       61 taggccaagg ggtaaaaagc gctgcacagc agcagagtga ggctaagcag gatgcggttc
      121 attgtgtact ccatggcaaa ataggtgtgc aggtgggctg ccaccttagc ttaatttacc
      181 cagggattta agtgcgctgg ccgcatcttg cgcatacagc ggccctctcg cgtgttgccg
      241 ccatgggggg caagttgtgc tgatgcttag gtgataaaaa aaattgatgg taaaagcctg
      301 gccaaagcct gcttgggcgc gtacaaagcg gccggtctta tttttgtaag tgagtgtgtg
      361 atgcatcttt cggtaactga actgtattta attgccatgt gcctgatttt ttccgtgcct
      421 tatttaattt ggcgcttggg ccgtaccgac tattgggcac ctttggtggt ggtgcagatt
      481 atttcgggca ttttattagg gcccggtgta atgggcgcct ggcaacctga gtattataaa
      541 tttgtgttta ccccggatgt tattaaatgc ctaaacggtg tggcctggtg ggccgtgatg
      601 atgtttgtgt ggattgcagg cattgaattg gatttaaaaa aggcctggca atatcgccgc
      661 gaaagcggca tcacagcggg cttagccttg ggtatgccgt tgctgtttgg ctgtgtggcg
      721 ggttttggat tgttgaatta ccaaggctgg atgggcagca aagcgctgac ttggcaattt
      781 gtattaggcg tgggtatggc ctgtgcggta acggcgctgc cgattcttat tttattaatg
      841 gaaaaattag atattttgcg cgcgcccatt ggccagcgca ttttgcgtta tgcaagcttg
      901 gatgatattg ccatttgggg tgtgcttgcg gttattttga tggattggga gcgggtaggg
      961 cgccaggtgg gatttttggt ggtgtttgcc gccgccgcat ttttattccg taaattaatg
     1021 tgtgctatcg aggaaaagga ccgctggttt gccggcatta tgtggctggc cctgtgtggc
     1081 ttaggcgctg attggtccgg cctgcatttt atggtggggg cgtttttagc cggtgcggtg
     1141 atggatgcgg attggtttaa tcaggaacat atggatttat tgcgccacca tttattgctt
     1201 acggtaatgc cagtattttt cttaagcacc ggcctgcgca ccaattggga tgtaggcggt
     1261 atggcggtat ttggcgtggc agctatttta ttggtagcct caattagcgg taaattagcc
     1321 ggtgtacatt tggccggtaa atggctgcgc tgggcgcccg gggaggcgtc tttaattggt
     1381 tggctgttgc agactaaagc cttaattatg attatttttg ccaacgtatt attggataaa
     1441 cagattatta ccaatgagac atttaccgcg ctgttattaa tggcggtggg cagcaccatg
     1501 ttaactacac ccgtagtggc gccgaaatta aaacgcttag gggcgttgct gcagcgggaa
     1561 acctaaggtc tgggcgcggt ttatgtgcgg cgcctagccc cttattaacc cggtactaat
     1621 tgccgccggg ttaataaaaa gattaaataa agcgttttgt acctatattt gcctgacttt
     1681 gtgtttgccc ataatcccct ctaaaaccct cctgtgcaca aatcacttcc ctgtgatatg
     1741 ccttaaaggc agcttcgcag tgcatattgc tcctgcaatt cgtgaaaagg ggagagggtt
     1801 ttatattggc agtgcaaaca atactccctg gcctactttt ttgtgtgcgg cccatttcat
     1861 tgggcaggta gtgaagccat ccctgcgcct gtaaaaatct acacttcttg ccgcagccct
     1921 attcgccagg cgacttctcg cccagccccc tatcagcgcg ataaattttt ttaactctcg
     1981 cttggcagtc agtgctgcta tggtgttcac agctggcttg gcttttaaat aaagcgagaa
     2041 ttcttattaa caacagcaga ggcgataatc tatgcacccg attgatatag gtattaacga
     2101 accccaacgc ctgcaaattg ctgagggctt aagccaatta ttggccgatt cctacacctt
     2161 gtatttgcaa acccacaatt tccactggaa cgtggtgggg ccgcattttc gcgaattaca
     2221 tttgatgttt caggagcaat acaccgagct tgccgtggcg gtggacgata tagccgagcg
     2281 cattcgcgct ttgggtgtgg cggcgccggg tacttatcag gcctttgcgc gcttaagcaa
     2341 aattaaagag gtggagggtg tgcccagcgc cagtgaaatg gtgcagatac tcaccacggg
     2401 ccacgagcaa attgtcaaaa cctgccgggc ggtgttgaag cgctcccagg cggcggatga
     2461 tgaatccacc tcctctttgg cgtccgaccg catgcgggtg cacgaaaaaa ccgcctggat
     2521 gttgcgggcg gtggccggat gattaggctt tgccgctgcg cgtgcggtgg ggtacgtaat
     2581 ggccattgtg ttgctccacc tgctgccgcg cccaggcggt aaattccccc agggaacaca
     2641 ggtggatgct gtataaaccc tcgccggcga caattttata aattactttg cgccgcatgg
     2701 ccttgggttc gtccaggtta atatccagca ccagccgcac cgcccagcta tcgcccaagg
     2761 cgccattgct gtagtagcag cctgcggcta tgggggcgtc caggtgtttt tgcgcctgag
     2821 cgtgttcgca ggctaggcgc accaactgct gtaagtcctc cgggcttacg tccaccaagg
     2881 tgtctatttc ggtaatcgcc gcggcaaaat cctcctgggc aaaactaaag ggcagggggc
     2941 tggtgggttt gctgtgccag gcgttggggt agcggcgcca ggggaagggg gcgttaaaca
     3001 ctagggcggc cagcagaata agcaccaggt tgagcaggca cacctgccac acaaaggccc
     3061 agcccgcgcc gccgccgatc accgccatca acgccgtggc gccccccggt gggtgcaggc
     3121 agcgcaaaaa atacatggcg gtaatcgctg cacctaccgc tagggcagcg gcgaattcac
     3181 cgggcagcca ctgctgcacc gccaccccaa tcaccccgct gattaaatgc ccacccacca
     3241 cgggccaggg ttgcgagagc ggtgcatggg gcagggcaaa cagcagcacc gccgaggcgc
     3301 ccatggaggg cagaataacc agcgccgcct ggggcagcag ccactgggtt cccagggcca
     3361 ctgtgctaat ggcaataaag gcgccgaggc cggaaagcca tttctcccgc tggcccagta
     3421 ggagtttaag aagccgggat gacgagttag aagtgctagt aggcatagct gcaccaaaat
     3481 agcgccaagg tacctttatg ctggtgggga ttcaactgcc ggctgccttc ctcatggtga
     3541 gcttgagcat tcatcgatta acccaagcgc cccagtcgtt tatgctgggc agattgttca
     3601 cttgtgagga ttacccatgc gccgccacct aacatggttg ttgttgttca ctttactggc
     3661 cccgcgcagc tgggccttgg agctacccag cctgcttgcc gattggcagg gctgggtgct
     3721 ggaggatgag gccgagcagt tttgccctaa ggcgctcgag gatgaccgta aagaatgcgt
     3781 ctggcccggc gagctgacac tgcaggccag cgacaaaggc gcggacttta aattagcggt
     3841 ggatgtgttt gcgcccagct gggtggacct gcccggcggg ccgggttatt ggccccagga
     3901 ggtgaaaaac ggcagtagtt ggattcccgt gcgggcaagc agcgagcggc cccaggtgta
     3961 cctgctcaaa ggccgccacc tgcttagcgg ccgcttcag
//
LOCUS       DAOQGS010000383         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12441_length_3991_cov_4.973577, whole genome shotgun sequence.
ACCESSION   DAOQGS010000383 DAOQGS010000000
VERSION     DAOQGS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12441_length_3991_cov_4.973577"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1931
                     /locus_tag="PKZ52_13280"
     CDS             264..1931
                     /locus_tag="PKZ52_13280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG60791.1"
                     /translation="MTDAPNAWREKYLDALDEHERLEARLDECQSALRRAYIHLSAAV
                     SGQNSELDTALLQARDRLKPGASSDFSAALQKVEKHLEALETFQAQQKNLGLSALLNA
                     CHSLRRLRLPKPALQNLNQFIQRLEREITNPAMLARALEDWQQLLEQALAAAALPDVS
                     LWQRLRGGRRLPADEAPAQSDFYQTIPAAPTNPAAEPDLQLDAVTHKCQVPIDQQPVA
                     EHIAAVLQQLLDLLNPTEALLPRVTDLRARIDSGLSWQQLGEALEELRDLYAQASSSL
                     DSELTDYLERVNDDLAQICQQLGAVLAVSNQSTALAEPLSQLAQSVKNATDLESLKRE
                     VSAQISGIHSALNQLANPWQEELPKLLERLQLMERQSQHTQKLLERERLRATFDSLTE
                     LPNREAFNQRIKLEWQRYKRYGQGLTLAICDIDHFKRFNDQFGHQVGDRVLKLVSKAL
                     AKRLREVDFVARYGGEEFVVILPQTPLPNALKTLDKIRAAIASLAFRFRDEPVQISLS
                     FGLAFSLDAETANQLFARADEALYAAKNAGRNCCIAWSKPPGETPEA"
     gene            2052..3326
                     /gene="traF"
                     /locus_tag="PKZ52_13285"
     CDS             2052..3326
                     /gene="traF"
                     /locus_tag="PKZ52_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNG60792.1"
                     /translation="MQPTRFTLSLLALSLMPLKLWAAGFGIYDARAMAMGGAATGVGD
                     THTGFFYNPALLALGEENENRARTSRWYFPMVSVQTTQASLDALDISEADLDTRLTGA
                     IDAFNAATGRDTASSALDITNEADDAIEKLTDHNLEVDAYAGFVVSIPSDYQGGALAL
                     GTHVVGGGLAFVAPDDRALLNKYRDFFTYASQNDTIDGAPHQELVDETTGKIGNPVSD
                     IASFVAFRGAVLTELSVSAGNLWSFGKADIALGITPKIVKALIFDEERRVVNEAVTTD
                     TQNSDHYYFDTDIGVLLQWQDKFRLGASIKDLRSKEFVSARGNPVAIKAKPRIGGAYV
                     DERYSLGLDLDLAHSSAFAGELTRQDLSLGGEYRWRSLALRGGYRYDISGNNSGSFSL
                     GAGVMLGRWVSDLALTQGGENLAAALQIGRAF"
     gene            3642..>3991
                     /locus_tag="PKZ52_13290"
     CDS             3642..>3991
                     /locus_tag="PKZ52_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60793.1"
                     /translation="MSQEAVKETRGFATEAKQLLHLMIHSLYSNKEIFLRELISNASD
                     AADKLRFEALNKPSLLEGDPDLRIRISYDKDARTITISDNGIGMSRDEAIINLGTIAK
                     SGTAQFLQNLSSDQ"
BASE COUNT          926 a         1336 c         1054 g          675 t
ORIGIN      
        1 ccaggctatc gaggagcggc gttaggggcc gttgcagagg tatttgttcg aacatgaggc
       61 agtacacaca taggatagac cccagcctgg cctgggcagc cggtcattgt atgtggcggg
      121 tgcaggcatg ggaagttgta aacggcagga tgttctgatt aataaccttg gggcccggcc
      181 aacccaaata ctgataggcc ggagaatata cgcactttgg cctacacttt aggcacctta
      241 agaattttcg gaaagcacga gccatgacgg atgcgcccaa tgcctggcgg gagaaatacc
      301 tggatgccct ggatgaacac gagcgcctag aagcgcgcct ggatgaatgc caaagcgccc
      361 tgcggcgagc ctatattcac ctgagtgcag cggtgtccgg gcagaactcc gagctggaca
      421 ccgccctgct acaggcccgg gatcgcctaa aacccggcgc cagcagcgac tttagcgccg
      481 ccctgcaaaa agtggaaaaa cacctcgaag ccttagaaac ctttcaggcc caacagaaaa
      541 acctcggcct tagcgcttta cttaacgcct gccacagcct gcgccgcctg cgcctgccca
      601 aaccggccct gcagaacctc aaccagttta tccagcgcct ggagcgggag atcaccaacc
      661 ccgccatgct cgcccgcgcc ttagaagact ggcaacagct gctggagcag gccctagccg
      721 cagccgccct gccggatgtg agcctctggc agcgcctgcg cggcggtcgc cgcctgcccg
      781 cagacgaagc cccggcccag agcgattttt accaaaccat cccggcggcc cccaccaacc
      841 ccgccgccga gccagactta caactggacg ccgtcaccca caagtgccaa gtgcccatcg
      901 accagcaacc ggtggccgaa cacatagcgg cggtgctgca gcaattattg gatctgctca
      961 accccaccga agccctgctg ccccgcgtta ccgacctgcg cgcccgcatc gacagcggct
     1021 taagctggca acaattgggg gaggccctag aggagctgcg ggatttatac gcccaggcct
     1081 catcatcttt agatagcgag ctgactgatt acctagagcg ggtgaacgac gacctagcgc
     1141 aaatctgcca gcagctgggc gctgtactgg cggtgagcaa ccagtccacc gccctggccg
     1201 aacccttaag ccagctggcc caaagcgtga aaaacgccac cgacttagaa agcttaaaac
     1261 gcgaggtgag cgcccagatc tccggcattc actcagcctt aaaccaactg gccaacccct
     1321 ggcaggagga gctgcccaag ctgctagagc gactgcaatt gatggagcgg cagtcccagc
     1381 acacgcaaaa actgctcgag cgggagcggc tgcgggccac cttcgacagc ctcaccgaac
     1441 tgcccaaccg ggaggccttt aaccagcgca tcaaactgga gtggcagcgc tacaaacgct
     1501 acggccaggg cctcaccctc gccatttgtg atatcgacca cttcaaacgc tttaacgatc
     1561 aattcggcca tcaagtgggc gatagggtgt taaaactggt cagtaaagcc ctcgccaaac
     1621 gcctgcggga agtggacttt gtggcccgct acggcggcga ggagttcgtg gtgatcctgc
     1681 cccaaacacc cctgcctaac gccctcaaaa ccctggataa aatccgcgcc gccatcgcca
     1741 gcctggcctt tcgttttcgc gatgaaccgg tgcaaattag cctgtcgttt ggcctcgcct
     1801 tcagcctgga tgccgaaacc gccaatcagc tgtttgccag ggccgatgaa gccctctacg
     1861 ccgccaaaaa cgcgggccgc aactgttgta ttgcctggag caaaccgccc ggcgagaccc
     1921 cagaggctta agctgcgggc caagcgcaca gtcttcagcc cccgccgccg ccttaactgg
     1981 ttaagcgcct gggcttttgg catcatggct cgcccgcgcc gcccttaaac ctaaccctac
     2041 aggtatcctg catgcagccc acgcgtttca ccctcagcct gctggcgctc agcctaatgc
     2101 cccttaagct ttgggcggca ggcttcggca tctacgacgc ccgcgccatg gccatgggcg
     2161 gcgccgccac cggcgtcggc gacacccaca ccggcttctt ctacaacccg gccctattgg
     2221 ccctggggga ggaaaacgaa aaccgcgccc gcaccagccg ctggtacttc cccatggtaa
     2281 gcgtgcaaac cacccaggcg agcctcgacg ccctggacat cagcgaggcc gacctggata
     2341 cgcgcttaac cggcgccatc gacgccttta acgccgccac cggccgcgac accgcctcct
     2401 ccgccctgga tatcactaac gaagcggatg acgccatcga aaaactcacc gaccacaacc
     2461 tagaagtaga cgcctacgca ggctttgtgg tgagtatccc cagcgactac cagggcggcg
     2521 cccttgccct gggcacccat gtggtgggcg gggggctggc gtttgtggcg ccggatgatc
     2581 gcgctttact caacaaatac cgggacttct tcacctacgc cagccaaaac gacactatcg
     2641 acggcgcccc ccaccaggaa ttggtggacg aaaccaccgg caaaatcggc aacccggtga
     2701 gcgatatagc ctcctttgtg gcttttcgcg gcgccgtgct taccgagttg agcgtgtcag
     2761 cgggcaacct gtggagtttt ggcaaggccg acatagccct aggcattacc cccaaaatcg
     2821 tcaaagcgct gatttttgat gaagagcgcc gggtagtgaa cgaagcagtc accaccgaca
     2881 cccaaaacag cgatcactac tacttcgata ccgacatagg cgtgctgctg caatggcagg
     2941 acaagttccg cctcggcgcc agcatcaaag acctgcgcag caaagagttt gtcagcgcgc
     3001 gcggcaaccc ggtggccatc aaagccaaac cccgcattgg cggagcctat gtggacgagc
     3061 gctacagcct ggggctggat ctggacctcg cccacagcag tgcctttgct ggcgagctca
     3121 cccgccagga cttaagcctg ggcggtgaat accgctggcg ctccctcgcc ctgcgcggcg
     3181 gctaccgcta cgacatcagc ggcaacaaca gcggcagttt tagcctcggc gccggggtca
     3241 tgctcggccg ctgggtgagc gatctggccc tcacccaagg cggcgaaaac ctcgccgccg
     3301 ccctgcaaat aggccgggcg ttctaacgcc ccaagcgttt aattaccgtc gccaaggtta
     3361 tttacctctc cccccaacac ccgccccgcc tcccccttaa atagggagag gggcaaaaat
     3421 acccgcaaaa aagcactttg cttacctgca aggcaatcag ccccctcccc tctttgaggg
     3481 gagggttggg gtggggtgag tagcagtaca tcgccaacca caaaacctcc cacgcaccca
     3541 ccaccccatt gaaatccccc gccaacagcc ccaataagag caccacgatt ttccagccgt
     3601 gcctggcaac ttaaaaaccc acaattagag gaaccctatc catgtcccaa gaggccgtca
     3661 aagagacccg tggatttgcc acagaagcca aacaacttct gcacctgatg atccactccc
     3721 tgtattccaa caaagaaata ttcctgcggg aattaatttc caacgcctcc gatgcggcgg
     3781 ataaattgcg ttttgaagcc ctcaataaac ccagcctatt agaaggcgac ccggacctgc
     3841 gcattcgcat cagctacgac aaagacgccc gcaccattac catcagtgac aacggcatcg
     3901 gcatgagccg ggacgaagcc atcatcaatt taggcaccat cgccaaatcc ggcacagcac
     3961 aatttttaca aaacttaagc agcgatcaga a
//
LOCUS       DAOQGS010000384         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12560_length_3967_cov_5.323108, whole genome shotgun sequence.
ACCESSION   DAOQGS010000384 DAOQGS010000000
VERSION     DAOQGS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12560_length_3967_cov_5.323108"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1428)
                     /locus_tag="PKZ52_13295"
     CDS             complement(238..1428)
                     /locus_tag="PKZ52_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60794.1"
                     /translation="MAFILCASTEELIANHPWEGVSVDRAYFYDLLVQTTLLGHVLVL
                     VAEEVFRHPVLRSELVDTNKSLARLLLAEGHIKILGELKEPPAVYHIHAKKISSIVNL
                     VRSSQAELPSPPSETIEQAFLQLLEQLPRQNSAEQGFYLGDIPEREYNLIINGFFKRV
                     ANGARPSRDLMLQLLNTLYGQTQGPWQSTGKRKLMQLLADLSSCALGRALQLTLRQRV
                     GIEGQFSPLIGGASATLAMHMAPLDGPVSLIKLPWPNSLLQPKTWQKALANEEFSRAR
                     NAFISALQASLQPPITRAQVDGLIHSAEEYQQLLQWFFAKSADKDMLANPANLVLSFE
                     PTSFAGSPVIGELYWQQAFNGFRRVMALWNFNGRQTGAVDARTLPSYQLIYNKKKLAA
                     IGLN"
     gene            complement(1707..2441)
                     /locus_tag="PKZ52_13300"
     CDS             complement(1707..2441)
                     /locus_tag="PKZ52_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNG60795.1"
                     /translation="MNSPEVAGNLVQQVTQSIGCAIVQGTFAKANAMITEAELSDRFK
                     ISRTVIREAVKMLAAKGLLTSRQRRGIRIAPSSQWNMFDEDVLQWTLSGAPSLHLLRE
                     FAQLRLAIEPEAAALASQCQDKAKIRDIQAAAEALMSVEQQGLSEYDADLHFHTMIFA
                     ATENRFFVQFRSFIQAALRVSIQCNFGIEGNKTAVADPHMQVYLAIEAGNAEMSRQLM
                     REMLVENLRLIDLAIAQQNTVAAVGA"
     gene            2619..>3967
                     /locus_tag="PKZ52_13305"
     CDS             2619..>3967
                     /locus_tag="PKZ52_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNG60796.1"
                     /translation="MTNPLAGQPLPKEQLVNVAHLLDAYVQRIPSADVSEQRVSFGTS
                     GHRGSSFNTSFNEYHVLAISQAICDYRNQANISGPIFVGRDTHALSEPAQQSALEVLV
                     GNNVTVMVAARNDYTPTPAVSHAILSYNQGRSTALADGIVITPSHNPPDNGGFKYNPP
                     NGGPADSDVTTWIQNRANELLADGLRAVKRASASEVRASNLVVAHDYIEQYVSHLPNI
                     IDLAAIAAAGVSLGVDPLGGAGLNFWYAIAERHKLNLSVVNPSVDPQFAFMSADWDGK
                     IRMDPSSQYTMRPLLKQAAAYDIVCACDADHDRHGIATAKGGLLPPNHYLAVMVDYLF
                     QSRPWRPEVAVGKTLVSSAMIDKVARRLGRPLLEVPVGFKWFAPGLFDGSLGFGGEES
                     AGASFLCRDGSVWSTDKDGLIPCLLAAEMTAANGQNPAQRYEQLCAEFGRSYETRIDA
                     "
BASE COUNT          917 a         1140 c         1045 g          865 t
ORIGIN      
        1 taattcccgc agtgatgaat acaggttatt tcagacacgc cgtgaataca tccgagtagg
       61 ctcgtcaaaa aggtccctct ttttgacggg ctgaaataac ctgtattcat cactgctgca
      121 cggcgaatca attctgccgc ttggctaaat cgaccttaat ttgccgccgg gttaataaga
      181 acataacaag gtgtgttaaa tagttaccga caaacaactc accgcaggta aatcccatta
      241 attaagccca atcgccgcca gttttttctt gttataaatt aattgataag acggcaaagt
      301 gcgggcgtcc accgcaccgg tttggcggcc attaaaattc cacagcgcca ttacgcggcg
      361 aaagccgtta aaagcctgtt gccaatacag ctcaccgata accggcgagc ctgcaaacga
      421 tgtcggctca aaactcaaaa ccaaattggc ggggttggcg agcatatctt tatcggcgct
      481 tttagcaaaa aaccattgta ataattgctg atattcctca gcactgtgaa ttaatccatc
      541 cacctgcgca cgggtaatcg gcggctgtaa actcgcctgc aatgcgctga taaaagcgtt
      601 gcgggcgcgg ctaaattctt cattggcgag ggctttttgc caggttttcg gctgcaataa
      661 actattgggc cagggcaatt taattaagga taccggcccg tctaagggcg ccatatgcat
      721 ggcaagggtc gcgctggcgc cgccgattag gggggaaaat tgcccttcga tgccgacgcg
      781 ctgccgcagg gttaattgca gggcgcggcc gagggcgcag ctgcttaaat ccgctaataa
      841 ctgcattaat ttgcgtttgc cagtgctctg ccaggggcct tgggtttggc cgtacagggt
      901 atttaataat tgcagcatta aatcgcggct gggccgtgca ccgttggcga cgcgcttaaa
      961 aaaaccgtta ataattaaat tgtattcccg ctcgggaata tcccctaaat aaaaaccctg
     1021 ctcagcgcta ttttgccgag gcaattgctc tagcaactgc aaaaaagcct gctctatggt
     1081 ttcgctgggc gggctgggta attccgcttg gctgctgcgc actaaattaa caatggagga
     1141 aatttttttg gcgtgaatgt ggtatacggc aggcggctct tttagctcgc cgaggatttt
     1201 aatgtgccct tctgccagta ataaacgcgc caggctttta ttagtgtcca ccagttccga
     1261 gcgcaatacc ggatggcgaa atacctcctc cgccaccagc accaacacat ggcccagcaa
     1321 ggtggtttgc actaacagat cgtaaaaata cgctcggtca acacttacgc cctcccaggg
     1381 gtggttcgca atcagttcct ccgttgaggc gcaaagaata aaggccatac tggctccaag
     1441 gttggccggg ctgctgatat agcggcgcac agggcgcgct aatttaacac gcttacaccc
     1501 cggcggtggt agcacagtac tgtcatcaag ttagtgattc tggcgctcaa tatcggtgtg
     1561 agatcacctt ggcagcctgg atctagcccg gtttcgaggc gccccggatt gcgctgcgct
     1621 gcatccgggc tactcaaacg gcgagctgta ttcttaaccc aatgccctat cgagaaatta
     1681 acaaccacgc ccagaagata gtaagcttaa gcccccaccg ccgcgaccgt gttttgctgc
     1741 gcaatagcca aatcaattaa gcgcaaattc tccaccaaca tctcgcgcat tagctgccga
     1801 gacatttccg cattgcccgc ttcgatggcc aaatatacct gcatatgcgg gtccgccact
     1861 gcggttttat tgccctcaat gccgaaatta cactggatac tcacccgcaa agcggcctga
     1921 ataaaactgc ggaattgcac aaaaaagcgg ttttccgtag cggcaaaaat catggtgtga
     1981 aaatgcaaat ccgcatcgta ttcgcttaag ccctgctgct cgacgctcat aagggcttcg
     2041 gcagcggctt gaatatcgcg gattttggct ttgtcttggc actggctggc tagggcggcc
     2101 gcctccggct caatggccag gcgcaattgg gcaaactcac gcaataggtg caaggagggc
     2161 gcgccgctga gggtccactg caatacgtcc tcatcgaaca tattccactg ggagctgggc
     2221 gcgatgcgga tgccgcgacg ctggcgggag gtgagcagac ccttggcggc gagcattttg
     2281 accgcctcgc gaatcaccgt gcggcttatt ttgaagcggt cggacagttc cgcttcggta
     2341 atcatggcat tcgccttggc aaaggtgccc tgcacaatgg cgcagccaat ggattgggta
     2401 acctgctgga ctaaattgcc cgcaacttct ggagagttca atggacaccg ttcctattta
     2461 aaaatcatcg cgtgcgcacc ggttacatcg gcggcgtttg aagggcgatt ctaatcaatt
     2521 gttcgacaat taacagtggg gagctgcgca aaacgcggtt gtgacttacc cacgcgcttt
     2581 gtaccatggg gcacacatca cgataatagg taggctgtat gacgaatcca ctggccgggc
     2641 agcccttgcc caaagagcag ttagtaaatg tcgcgcactt gctcgacgcc tatgtgcagc
     2701 gcatccccag cgccgatgta agtgagcagc gggtgagctt cggcacctcc ggccaccgcg
     2761 gcagttcgtt taacactagc tttaatgaat accatgtgct ggccatcagc caggccattt
     2821 gcgactaccg caaccaggcc aatatcagcg ggcctatttt tgtcggccgc gacacccacg
     2881 ccttatccga gcccgcccaa caaagcgccc tggaggtgtt ggtgggcaac aatgtgacgg
     2941 ttatggtggc cgcccgcaac gactataccc ccacacccgc cgtctcccac gccatcttga
     3001 gttacaacca gggccgcagc acagctcttg ccgatggcat agttatcacc ccctcccaca
     3061 acccgccgga taacggcggc tttaaataca acccgcccaa cggcggcccg gccgatagcg
     3121 acgtcaccac ctggattcaa aaccgcgcca atgaattgct tgccgatggc ttgcgcgcgg
     3181 taaaaagggc cagcgccagc gaggtgcgcg cctccaacct ggtggttgca cacgattaca
     3241 ttgagcaata cgtcagccac ctgcccaaca ttatcgacct tgccgccata gccgccgcag
     3301 gcgtaagcct gggagtagac cctttagggg gcgcggggtt gaatttttgg tacgccatcg
     3361 ccgagcgcca caagctcaac ctcagtgtgg tcaaccccag tgtggacccg caattcgcct
     3421 ttatgagcgc cgactgggac ggcaagatcc gcatggatcc ctcctcccaa tacaccatgc
     3481 ggccactgct aaaacaggcc gccgcctacg atatcgtctg cgcctgcgac gccgaccacg
     3541 accgccatgg catagccacc gcaaaaggcg gcctgttgcc ccccaaccac tacctggcgg
     3601 ttatggtgga ttacctcttt cagtcccgcc cctggcgccc tgaggttgcc gtgggtaaaa
     3661 ccctggtgag cagcgccatg atcgacaagg tggcccgccg cctaggccgc cccttgctgg
     3721 aagtgccggt gggctttaaa tggtttgcgc cggggctgtt tgacggcagc ctcgggtttg
     3781 gcggcgaaga aagcgctggc gcgtcttttt tatgccggga cggcagcgtc tggtccaccg
     3841 ataaagacgg cctgattccc tgcctattag cagcagagat gaccgccgcc aacggccaaa
     3901 acccagccca acgctatgag caactgtgcg ctgaatttgg ccgcagctat gaaacccgca
     3961 tcgatgc
//
LOCUS       DAOQGS010000385         3964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12581_length_3964_cov_4.829880, whole genome shotgun sequence.
ACCESSION   DAOQGS010000385 DAOQGS010000000
VERSION     DAOQGS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12581_length_3964_cov_4.829880"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..315)
                     /locus_tag="PKZ52_13310"
     CDS             complement(136..315)
                     /locus_tag="PKZ52_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60797.1"
                     /translation="MADEGVWHDTSGGVPLGYGSCIHPVEGGPLSLLLSITVLYLERE
                     KRKHTFARYADDLMI"
     gene            complement(338..1597)
                     /locus_tag="PKZ52_13315"
     CDS             complement(338..1597)
                     /locus_tag="PKZ52_13315"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNG60798.1"
                     /translation="MNTPPAGPAVEVGVRLAVDQVARSAYGRLVAWLAVQWRDIAAAE
                     DALADAFVKALQTWPRDGIPTHPDAWLLTVAKRELLQKHRREKFLQDSRFTAMFSDIS
                     DEGGIEHLAQALPDYRLSLMLTCASPAIEETVRMPLMLQTVLGLRVDEIAPALLLSPG
                     SLAQRLVRAKLRIKNQALPFIPGDAADLPEALHCVLEAIYGTFGISSDDVDGAEIRSQ
                     GLQQEAIYLCQLLCELLPQSAEAKGLLALMYFCRARRAAQTAEEHVFIPLAEQNKALW
                     DRALIQTADHLLMQAHGLRQPGYFQLEAAVQSAHCHRLFSGQTPWHAIAELYRQINSY
                     FPSLGSQVAGAVACAEAGKLPEAWDLLHQIDPARVRSFQPWWVAKAHLATLMGDKIAA
                     YAAYTHAIGLSQQKKIIAHLTQKRDGL"
     gene            complement(1604..1963)
                     /locus_tag="PKZ52_13320"
     CDS             complement(1604..1963)
                     /locus_tag="PKZ52_13320"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNG60799.1"
                     /translation="MQCMMIFKESTEEVNKRHDPEAAPAYWAGWMAYIGALSESGLVL
                     SGNGLQHPSTGVTLSIRDGQRQIMDGPYADTKEHLGGYFILEVESLDVALDWAARSPA
                     ALNGSVEVRPVLPPPAP"
     gene            2224..3028
                     /locus_tag="PKZ52_13325"
                     /pseudo
     CDS             2224..3028
                     /locus_tag="PKZ52_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644283.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            3081..3698
                     /locus_tag="PKZ52_13330"
     CDS             3081..3698
                     /locus_tag="PKZ52_13330"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HNG60800.1"
                     /translation="MHYFLLLCALLLPGCDKKPTNHAAIKPSPLVVIKIPAAMEHTQE
                     KAEPAPEGKHTQNTHAHGAAKLQIIAEGNLLSVDMSMPSSDAVGFEHAASNATEKAQL
                     TATLNTLQAPDYLFELSPTAGCTLKQGSVATALLNPQPAPAEHADFDAHYEWECTNAA
                     RIKNISIKLFTYFISLEKITADYIVDTKQGGIELRRNGQTQINFD"
     gene            complement(3716..>3964)
                     /locus_tag="PKZ52_13335"
     CDS             complement(3716..>3964)
                     /locus_tag="PKZ52_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNG60801.1"
                     /translation="EYNAEQLLTEFFLVPYFGACIHVPPPPPNQIIYVRLPKGLPPPD
                     IYTPFWLQGTLKIETKENDLGVSSYSLDATSVEAYTEE"
BASE COUNT         1031 a         1050 c         1009 g          874 t
ORIGIN      
        1 aatattctcg ccgaatgcac tgtacaggac gggttgcaac ccgcccttgt atcaccgcac
       61 ttttaaatta agcctaaggc caacaaaccg cgcatcactg cacaatacgc gctcccctgc
      121 tcgtatactc ccgactcaaa tcattaaatc atcggcgtag cgcgcaaagg tgtgtttgcg
      181 tttttcccgc tctaaataca gcacagtaat agacaacagc agcgagagtg ggccaccttc
      241 gactgggtgg atgcaggagc catagccgag cggtaccccc cctgaagtgt cgtgccacac
      301 accttcgtcc gccatgccag ggcgaagaat tccgccccta aagcccatca cgcttttgcg
      361 ttaaatgcgc aatgattttt ttctgctggg atagccctat ggcgtgggta taggccgcgt
      421 aagcggctat tttatcgccc attaatgtcg ctagatgggc ctttgcaacc caccagggct
      481 gaaagctgcg caccctggca ggatcaattt gatgcaataa atcccaggcc tcgggcagct
      541 tgcctgcctc agcacaggca acagcccccg ccacctggga cccaagcgat gggaaataac
      601 tgttgatttg ccggtataac tcggcaatgg catgccatgg tgtttggccg gaaaacaacc
      661 ggtgacagtg ggcggactgc acggccgctt ccaactgaaa atacccaggc tggcgcaaac
      721 catgggcttg catcagcaga tgatcggcgg tttgaattaa cgctctgtcc caaagggcct
      781 tattttgctc agccagggga ataaacacat gctcttctgc cgtttgggca gcgcggcgcg
      841 cgcggcaaaa atacatcaat gccaataaac ccttagcttc ggcggattgt ggcaatagct
      901 cacacagcag ctgacaaaga taaatcgctt cttgctgcaa accttggctg cgaatttctg
      961 cgccatcaac atcgtcactg cttatgccaa aggtgccgta aatggcttcc aacacacagt
     1021 gcagcgcctc gggcaaatca gcggcatcgc ccggaataaa cggcaaggct tggtttttaa
     1081 ttctcagctt ggcgcgtacc aaccgctgcg ctagtgaccc aggcgagagc aatagcgccg
     1141 gggcaatttc atcaacgcgc aagcccaaga ctgtctgcaa catcagtggc atgcgcaccg
     1201 tctcctcaat ggcggggctt gcacaggtga gcatgagcga taaacgatag tccggcagcg
     1261 cctgggcgag gtgctcaatc ccaccctcat cgctgatgtc gctgaacatg gcggtgaaac
     1321 gcgaatcttg cagaaatttt tcgcgccgat gtttttgtag caattcgcgc ttagcgaccg
     1381 tcagcagcca ggcgtccgga tgggtcggga taccatcccg gggccaggtt tgtagcgctt
     1441 tcacaaaggc atcggccaat gcatcttcgg cagcagcgat atcacgccat tgcacggcaa
     1501 gccaggccac caggcgcccg taagcactgc gggcaacctg gtcaacagca agcctgaccc
     1561 ccacttcaac ggccgggcca gcgggtggcg tattcatacc ggtttatggc gccgggggcg
     1621 gcaacaccgg ccgcacctcc acactaccat ttaacgccgc cgggctgcgg gcagcccaat
     1681 cgagggcgac atctagggac tcaacctcca aaataaagta accgccaaga tgctccttgg
     1741 tatcagcgta agggccatcc ataatttggc gctggccatc gcggatactg agcgtgaccc
     1801 cggttgaagg gtgttgtaag ccattaccac ttaacaccag gccagattcg ctaagggcgc
     1861 cgatataggc catccacccg gcccagtatg ctggcgccgc ctcaggatca tggcgtttgt
     1921 tcacttcctc ggtggactct ttaaaaatca tcatgcactg catatggctt tctccaatgt
     1981 attttcgatt aataggtaac aaaggccgcg tgttgtgtat caatcgatca ggggcagcct
     2041 tgtcgttaac tagatgcatg gcaaattcgt caatcgacag gggcgaaaaa aatatttacg
     2101 cataccaaga gcattccgca agttcatagc cttaggtaca gccagggtgc aggctacggc
     2161 gcgccctggc cttggggtgt ggcatttgca cctgagggac aaaaataacc tagcctaaac
     2221 ctagtggtag atggggtgaa ttctcttaaa tgattatgac aaggcattcc ccttggagga
     2281 gaccggcgaa tccttagggg aaaatgacga gcgcacggtg gcggacctat taattgagca
     2341 ggtggaattt tgcgacgtat tattaattag caaaaccgat ttaattagcc ctgagcgcgt
     2401 agcggaatta acagccgtac tgcgcaaact taatagccgg gcagagatta tccccatgag
     2461 taaaggcaat gtgcctttgg caaaagtgct caatacaggc cgctttgatt ttttacaggc
     2521 ccaattggcg ccaggttggc ttaaggagat gcgcggcgaa cacacaccgg agacagaaga
     2581 atatggcatt agcagttttg tctaccgtgc ccgcaagccc ttccatccac aaaagattta
     2641 tgattttttt agcaattccc aaacggtagc caaaaatcta ttgcgctcta aagggtattt
     2701 ttggctcgca tcccggcccc actacgcagg ccaatggagc caagccggtg gcattgccca
     2761 gcacggcgct gccggtttat tttggcacgc ggtgcccaaa gagcagtggc cggatgatgc
     2821 agaaaccgtg cagtatattc tggaaaaatg gcaagagcct ttcggcgata ggcggcaaga
     2881 attagtattt attggccgga atctggatca agcagccttt attgccgacc tggatgcatg
     2941 cctacttacc gatgaggaaa ttgctcaagg cgaaacctat tggcaaactt tgccagaccc
     3001 ttttcctagc tggagcgaaa aacattaacc ggccataagg cgcaggtaaa aagactgaag
     3061 cctttttaac gagaaaatcc atgcattatt ttttattgtt atgcgcactg ctgttaccag
     3121 gctgcgataa aaaacccacc aaccatgcgg caattaaacc ctcgccattg gttgttatta
     3181 aaatacccgc agcgatggag cacactcagg agaaggccga gccggcgcca gagggcaagc
     3241 acacgcaaaa cacccacgcc cacggcgcgg caaaactgca gataattgcc gagggcaatc
     3301 ttttaagtgt tgatatgagt atgcccagta gcgatgcagt agggtttgaa cacgccgcca
     3361 gtaatgcaac tgaaaaagca caattaactg ccactttaaa cacactgcaa gcgccggatt
     3421 atttattcga acttagccct acggcaggtt gcacattaaa acagggctcg gtggcaaccg
     3481 cactgctaaa cccgcaacca gcacccgctg aacatgccga ttttgatgcc cactatgaat
     3541 gggagtgcac aaatgccgcg cggatcaaaa atattagcat caagctcttt acctatttca
     3601 taagtctgga aaaaataacg gcggactata ttgttgacac caaacaaggg ggcattgagt
     3661 tacggcgcaa cgggcaaacc cagataaatt ttgattaggt gtgattattt ttacgttatt
     3721 cttctgtata ggcttccaca ctggtggcat ctaaactata ggaggataca cccaagtcat
     3781 tttctttagt ttcaattttt aaggtgccct gcaaccaaaa cggtgtgtaa atatccgggg
     3841 gcggcaagcc cttgggaagg cgcacataaa taatttgatt gggcggtggc ggtggcacat
     3901 ggatgcaggc gccaaaatac ggcaccaaaa agaattccgt caacaattgc tcggcgttgt
     3961 attc
//
LOCUS       DAOQGS010000386         3963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12587_length_3963_cov_4.936029, whole genome shotgun sequence.
ACCESSION   DAOQGS010000386 DAOQGS010000000
VERSION     DAOQGS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12587_length_3963_cov_4.936029"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(722..1516)
                     /locus_tag="PKZ52_13340"
     CDS             complement(722..1516)
                     /locus_tag="PKZ52_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60802.1"
                     /translation="MNRLQNALTAALLFGLAGTAHAKTYTYELTNLNSSNSQIIYPGL
                     DLNGAKSATFTVNRNGNELPEIRSFEITFPNAGKLRTGAFKRLEDGRYRALVSGAWVF
                     KEVIIQLDGNSDFVTNSFAHIQIFVSETTSNLNPEALNQGPILATAGGVVRETTNLVT
                     VDSTAIMIDGKRLSLNLKDRLGQSPDGPQGFILDALWMGRGQKLIYIPGPFAPDRWDR
                     IEPIAIVIDGPATDPIVQIRYKDNGSEQMSFSMPLRALLQQNFPTP"
     gene            complement(2110..3690)
                     /locus_tag="PKZ52_13345"
     CDS             complement(2110..3690)
                     /locus_tag="PKZ52_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNG60803.1"
                     /translation="MQRISLLFLSCLLTTACGGNKPPGTTSGGTSSSSSSSSSSSSSS
                     SSSSSSSSSSSTSSSSSSGATGWQLVWADEFSGSTLDTSKWSFEKNCAGGGNNELQCY
                     TTAAENAYVKDGYLNITALKKTTQGPAKFDDDSAYNPQDTSVTRDYSSARLRSKNKGD
                     WRYGRMDIRAKLPQGQGIWPAIWMLPTASAYGAWPLSGEIDIMEAVNTNAAGGNAVYG
                     TLHYGNPWPNNKYTGANTSPATPIWQEFHTYSVEWEAGEIRWYVDNNHYATQVQSGWF
                     TAATSKPGAPFDQAFHMILNLAVGGEWPGSPNAQTTFPQTMQVDFVRVYQCAQDTDTG
                     KGCASFINPTIKPLTGNQPPAATPGRANYALPPMFTLFNDSLASGLQYGSYNPDNTIS
                     ISEANETGRGKIIKIAKTGSNGNVFLNLIDGPVDLRSFAEGGEIRFDLKINSLAAGSK
                     LLIKLDSGWPNTSDIAVNFPATGQWAEVRISIPQLIARGNSLAAGAANLASVTNLLVI
                     EPTGDMDLSLDNIQLVKP"
BASE COUNT          906 a          804 c         1085 g         1168 t
ORIGIN      
        1 ctctcgggtt attctaaccg tttttagggt gtccactttt agtggtaggg atcaagctac
       61 tgcttgctgc ccaacaggat tgattggaca ggtaaagtgc gcagcttttg ttgttagata
      121 tccagtcgag agaagttttc ttcgggttta ctcgtgttca tttctgctcc tgaatgttta
      181 accggctggt gcctgcgcca aataaatatt attctcataa taaacaatag ttaataaaaa
      241 atataatata gaatttttgg aatcctaggc tatcgatggt tgaatttttt tggtgcggca
      301 gtcctaattg tgccttcgtg tggaatatcc caattttggc ttttgtatta cggaatatag
      361 ataatgtgct ctggttttag gtgggagcat cctgtgtgtt ctgtaattcg attatagcgg
      421 tatggttgct agaaattcgt ggttgtgtag aggctgattt tggggtgcag aatatgtggt
      481 gagcaggatt aataaatggc aatatgacaa cctcgattat gagattgttg tttaattgta
      541 tatccgcaat aaaaatcaaa gggatgctag gttgtaagat atggtgcttg aaattcgatc
      601 gctgaaatta tgcgctatgg atgtgggaaa aaacagcgga aaattttata tgtttttcga
      661 gaaagtttgt ctacatagat atagaaaccc tggcacacat tagcatacca ggggaaaacc
      721 attaaggcgt tggaaagttt tgttgcaata gtgcacgcaa tggcatcgag aacgacattt
      781 gctcggatcc gttatcttta tagcggatct gcactattgg atctgtagct ggcccatcta
      841 taacaatagc aatgggttca atgcgatccc atcggtctgg cgcgaaaggt ccggggatat
      901 agatgagctt ttggccgcgt cccatccaca atgcatcgag aataaacccc tgcgggccgt
      961 caggagattg tcccagccga tcttttaggt tgagtgatag acgtttgcca tcaatcataa
     1021 ttgcggtgct gtccacggta actaagttag tggtttcgcg gacaactcct ccggctgtgg
     1081 ctagtatcgg gccttggttc aatgcttctg ggttgaggtt ggaggtggtt tcactcacaa
     1141 aaatctgtat atgtgcaaag ctattggtaa caaagtcaga gttgccgtct aactgaataa
     1201 ttacttcttt aaacacccaa gcgcctgaaa ccaatgctcg gtagcggcca tcttctaagc
     1261 gcttaaatgc gccggtccgc agcttgccag cgtttgggaa agtgatttca aagctgcgaa
     1321 tttctggtaa ttcattaccg ttgcgattta ctgtaaacgt ggctgatttt gcaccgttta
     1381 agtccaggcc tggataaatt atttggctat tggatgagtt gagattagtt aactcatagg
     1441 tataggtttt tgcgtgtgcg gtgccggcaa gtccgaagag gagggcagct gttaaagcat
     1501 tttgtagtct gttcatattg attcctacag ttggttccta agttaagtgc acattttggc
     1561 gttaattgcc accggtgcaa gcacaagtgc tcgcccggcg ccgtacgcta aatctattta
     1621 atccgtatgt caattatttg atgggtttag acctgatatg ttttgaggtg ttgagtttta
     1681 gaatggcttc tatttttaac tagaatgatt tgaacatcca agttttgtag gtttattaaa
     1741 tattttgagg cttgagttgc ctatcgatta gttgacaagc aggttaagtg ctaggctgta
     1801 cattgaaagt attcaaatgt cttgaatggt agtttttatg tcgctgagaa tacgaatgta
     1861 tcttgaggag gctttagcga ctatgattta atcggtttga aatgctgaat aggaggcaat
     1921 gaattaggta ttgatggctt aactaacacg cagtttgatt ttttgctggc cagagttggc
     1981 gtgggtgcaa tcagcggtta atactctgcg tgctttgttg atcttgtatg cgagcgtaag
     2041 gcccgcattc aaaatcgctt tagaagctgg aattgtttga gtcccagatg ttcaactagt
     2101 gattaatttc taaggtttca ctaattgaat attatctaaa ctcaaatcca tatcccccgt
     2161 gggctcaatt accaacaggt tggttaccga agccagattc gcagcccccg ccgctagcga
     2221 attcccccgc gcaattaact gcgggattga aatccgtacc tcagcccatt gccctgttgc
     2281 aggaaaatta acagcaatat ccgaggtatt gggccagccg ctgtctagtt tgattaataa
     2341 tttactacct gcagccagtg agtttatttt taaatcaaaa cgaatttccc ctccttcagc
     2401 aaaactgcgc aaatccactg gcccgtcgat taaatttaaa aacacattgc cattgctacc
     2461 ggttttagca attttaatga ttttgcctcg gccggtttcg ttagcttcgc tgatgctaat
     2521 agtattgtcc gggttatagc tgccgtattg taggccgctg gctaggctgt cgttaaatag
     2581 cgtaaacatc gggggtaggg cgtagttggc gcggccggga gttgcggcgg ggggctggtt
     2641 gccggtgagg ggttttatgg tggggttgat aaaacttgcg cagcctttgc cggtgtcagt
     2701 gtcttgggcg cattggtata cacgcacaaa atccacctgc atggtttgcg ggaaggtagt
     2761 ttgggcattg gggctgccgg gccattcgcc gcctacggcc aggtttagaa tcatgtggaa
     2821 ggcctggtca aacggcgcgc cgggtttgct ggtggcagcg gtgaaccagc cgctttgtac
     2881 ttgggtggcg tagtggttgt tatccacata ccagcggatt tcgcccgctt cccactccac
     2941 cgaataggta tgaaattcct gccagatggg ggtagccggg gaggtgttgg cgccggtgta
     3001 tttgttattg ggccaggggt tgccgtagtg cagggtgccg tacacggcgt tgccgccagc
     3061 ggcgttagtg ttaaccgctt ccataatatc gatttcgccg cttaggggcc aagcgccata
     3121 ggcggaggcg gtgggcagca tccatatggc gggccatatg ccttggccct ggggtagttt
     3181 cgcgcggatg tccatgcggc catagcgcca gtcgcccttg tttttactgc gcaaacgagc
     3241 cgaggaatag tcgcgagtga ccgacgtatc ctgggggtta taggctgaat cgtcgtcgaa
     3301 tttggctggc ccttgggtgg tttttttcag ggcagtgata ttcaaataac cgtcttttac
     3361 gtaggcgttt tccgctgctg tggtgtaaca ctgcagttca ttattgccgc cgcctgcgca
     3421 gtttttttcg aatgaccatt tgcttgtgtc tagggtagag ccggaaaatt catcggccca
     3481 aaccagttgc cagccggttg cgccggatga gctgctactg gatgtgctgc tggagcttga
     3541 ggagctactg cttgaggaac tgctggatga gcttgagctg ctggacgaag agcttgagga
     3601 ggtgccgcct gaagtcgtgc ctgggggctt gttgcctccg caagcggttg tgagtaggca
     3661 ggataaaaac aacagtgata tgcgctgcac ggttcggccc tcgagcggta aaaaacaacc
     3721 ccggcaaggg ccggggttaa atcgttattt aagcagttta gcgcgcggca agtgttgcta
     3781 tggttccgct tttagtctgg gcggcaatgg tagcggggga tggatttcag cactttctcc
     3841 ccgtggcgat atttaacgct gtagcagttg ccgtattcat cccgcagggt ttcatcgtaa
     3901 taagcgcgct caaccggctt gcgtttgggg tactggtaac gcggtgccga ataggtcacc
     3961 ggg
//
LOCUS       DAOQGS010000387         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12606_length_3961_cov_3.986431, whole genome shotgun sequence.
ACCESSION   DAOQGS010000387 DAOQGS010000000
VERSION     DAOQGS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12606_length_3961_cov_3.986431"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PKZ52_13350"
     CDS             <1..1169
                     /locus_tag="PKZ52_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNG60804.1"
                     /translation="AQRIEQRTKYDLEMMQELGYCTGIENYSRYLSGREPGLPPPTLF
                     DYLPAEALLVIDESHVTVPQIGAMYKGDRSRKETLVQYGFRLPSALDNRPMRFEEWER
                     LAPQMIFVSATPGKYEEEHQGQVVEQVVRPTGLIDPIVEVRPASTQVDDCLSEIRRIA
                     DRNERILVTVLTKRMAEDLTDYLIEQNIRVRYLHSDIDTVERMEIIRDLRMGEFDVLV
                     GINLLREGLDMPEVSLVAIFDADKEGFLRSDRSLIQTIGRAARNVHGHAILYADKITA
                     SMQRAMDETQRRREKQMAFNELHGITPKGIIKNVADILEANRIPGARTGRSKKAAKNS
                     PEEILQKIDPYKDLWGQIEQLEKSMQQAAKDLEFEKAAALRDAIAQLKVRAMAS"
     gene            1229..1498
                     /locus_tag="PKZ52_13355"
     CDS             1229..1498
                     /locus_tag="PKZ52_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG60805.1"
                     /translation="MTVWIDVRAADEFAAGHISGALLIPHEEIAAKIGFFDFAKDEDI
                     RVYCRTGRRSGIAKEVLNNLGYVNVINEGGYEELLQRKAQGQPIP"
     gene            1794..3017
                     /locus_tag="PKZ52_13360"
     CDS             1794..3017
                     /locus_tag="PKZ52_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNG60806.1"
                     /translation="MNNILFNTHDIVLAVCILFCAFFAVANNLSKTFSPASRNLLTVF
                     FVLSACASFDTLVFWGDAVRYAAFALSPWLLMLFSFAAFAIGPFLYWFFRSLQQPEVK
                     LSATDYLQLLPALLALPYLYWACLRHPIEQQQALILNFSIFSNTQVHFFAFLTLKKLI
                     PVVYGIQCLALIYRKPSLMAGQPAALRPILQLYCGFVVLWVWGLAVHLLGQWMPVKLS
                     DQLGVAGNYLSLALLLVIWLERSKMPALIERIQLAQLVAAPQPSEHLPATEDAKDSEV
                     DELAARIQGLMLEQQPHLNSQITLERFAALLGEPPRPVSVAINRCFKQNFQEFINCFR
                     IEEAKRRLQDPLLADCTVVEIAHQAGFNSKATFNRLFKQQVGVTPSAYRQAFLPSFMV
                     SRHPFAASQDLHNLN"
     gene            3110..>3961
                     /locus_tag="PKZ52_13365"
     CDS             3110..>3961
                     /locus_tag="PKZ52_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNG60807.1"
                     /translation="MLTTKKWLGAALAALCLTAGVSETALAKPYKSAEIFTPAANLYG
                     KYVVRMRAAKASGVISNFFLWKDGSEMSGVFWEEVDVEVFGKNNATSWQSNIITGQGT
                     RTTSEQVHNSGSSFGDGYNTFTLEWSPDKVRWLVNNQVIRTTEGGQARNLLSPSQFRF
                     NFWPPNNPEWVGSWNDSVLPQYMFVNWIEFHRWNGSGFELAWRDDFNSFDSNRWGKAD
                     WTFTENRADFSPNNVVVKNGYLVLAMTREGQEGYNGTPPVDNGGSNSSSSSSSSSSSS
                     SSSSSSSS"
BASE COUNT          928 a         1057 c          999 g          977 t
ORIGIN      
        1 aggcccagcg cattgagcag cgcactaaat acgatttgga aatgatgcag gagttgggtt
       61 attgcaccgg tattgaaaac tattcccgct atttatctgg ccgggaaccg ggtttaccgc
      121 cgcccacttt atttgattat ttacctgccg aagcactttt ggtaattgat gaatcccacg
      181 taaccgtgcc gcaaattggc gccatgtaca aaggcgatcg ctcccgcaaa gaaacccttg
      241 tgcaatacgg ttttcgtttg ccctcggcgc tggataaccg ccccatgcgg tttgaagaat
      301 gggagcgctt agcgccgcaa atgatttttg tctcggccac acccggtaaa tatgaagagg
      361 aacaccaggg ccaggtggta gagcaggtgg tgcgccccac ggggcttatc gatccaattg
      421 tagaagtgcg gcctgcttcc acccaggtgg atgattgttt atcggaaatc cgccgcattg
      481 ccgatcgcaa cgagcgtatt ttagtaaccg tactcaccaa acgcatggcg gaggatttaa
      541 ccgactattt aatcgagcaa aatattcgcg tgcgttattt acactcggat atagacacgg
      601 ttgagcgcat ggaaattatc cgcgatttac gtatgggcga atttgatgtg ctggtgggta
      661 ttaacctgtt gcgggagggc ttggatatgc cagaggtctc cctggtggct atttttgatg
      721 cggataaaga aggctttttg cgttcggatc gctcattgat tcaaaccatc ggccgcgccg
      781 cgcgcaacgt gcatgggcac gctattttat acgccgataa aattaccgcg tccatgcagc
      841 gggccatgga tgaaacccag cgccgccggg aaaaacaaat ggcatttaat gagttgcacg
      901 gcattacacc caaagggatt attaaaaatg tggcggacat tctagaggcc aaccgcatac
      961 ctggtgcccg caccggccgc agtaaaaaag cggcaaaaaa ttccccggaa gaaattttac
     1021 aaaaaattga cccctacaaa gatttgtggg gccaaataga acaattggaa aaatccatgc
     1081 agcaagcggc taaagatctt gagtttgaaa aagccgctgc cttgcgcgat gctatagcgc
     1141 aacttaaagt gcgcgccatg gccagttaac gcctaatttt gtacaccttc acccatatta
     1201 tgaataacat aactttgagg caactcacat gacagtatgg atcgacgttc gcgccgccga
     1261 tgaattcgcc gcaggccata tctccggcgc cctattaatt ccccatgaag aaatcgccgc
     1321 taaaattggc ttttttgatt ttgccaaaga tgaggatatt cgcgtctact gccgcacggg
     1381 ccgccgctcg ggcattgcta aagaagtgtt aaataatttg ggttatgtaa acgttatcaa
     1441 tgagggcggc tacgaagaat tgttgcagcg caaagcccag ggccagccga ttccttaatc
     1501 gcagtgagtt tttaaaccat cgcccgttgg caaattgcgg ttgaatttaa aacttcaatg
     1561 agtgcattcg gctgagatct gcagcacatt aattatacgc tggcaattcc cctgtgacgg
     1621 ccgtcaacga ccctcgcggc ggggtttgcc attctaggcc taatagtcag ttccacgaga
     1681 agctgcagca catagcttgg cagagcgtta acaccgaacg ggcgagtggg taaagtggtg
     1741 cggccctttc gcgcccttta cattggctgg agcccttaag ggtttgaagg cgtatgaaca
     1801 acattctttt taatacccac gacattgtgt tggccgtttg cattctattc tgcgcatttt
     1861 ttgcggttgc caataatctc agcaaaacct tttcccctgc ctcgcgcaat ctgctcacgg
     1921 tattttttgt tttaagcgcc tgcgcctcct tcgacacctt ggtgttctgg ggcgatgcgg
     1981 tgcgttatgc agcctttgca ctttccccct ggctgttgat gttatttagt tttgccgcct
     2041 ttgccatagg gccttttttg tactggtttt ttcgctcctt gcagcagcct gaggtaaaac
     2101 ttagcgctac ggattacctg caattattgc ctgcgctcct ggcattgccc tatctgtatt
     2161 gggcctgctt gcgccacccc atcgaacaac agcaggcact tattttaaat ttctccattt
     2221 tcagtaatac ccaggtacat ttttttgcct tcctcacctt aaaaaaactt atccccgtgg
     2281 tgtatggcat ccagtgcctg gcgctgattt atcgcaaacc gagtttaatg gccgggcagc
     2341 ccgccgcgct gcggcctatt ttgcagttgt attgcggctt tgttgtgctc tgggtgtggg
     2401 gcctggccgt gcatctgctc ggccagtgga tgccggtcaa attaagcgat cagcttgggg
     2461 tggcgggcaa ttatttaagc ctggctttgc tgctggtgat ttggctggaa cgctcaaaaa
     2521 tgccagcgct cattgagcgc attcagctgg cccagctggt agcagcgccg cagcccagtg
     2581 aacacctgcc cgcaactgaa gacgctaaag atagtgaagt ggatgagttg gccgcgcgca
     2641 tacaagggct catgctggag cagcaacccc acctgaattc ccaaattacc ctggagcgtt
     2701 ttgccgcttt gctgggcgag ccgccgcggc cggtgtcggt ggccattaac cgctgtttta
     2761 aacagaattt tcaagagttc attaattgct ttcgcatcga ggaagccaag cggcgcctgc
     2821 aggatccgct cttggccgat tgcacggtgg tggaaattgc ccatcaggcc gggtttaaca
     2881 gcaaggctac ctttaaccgc ctattcaaac agcaggtggg tgttacaccc agtgcctacc
     2941 ggcaggcgtt tttgccaagt tttatggtct cgcggcaccc ttttgcagcg tctcaagatc
     3001 tgcacaacct aaattgaagc tttaccgcct ctattaagcc cttcaatctg ccatttgtgc
     3061 cgcgcccgtg aggcgcggcc tcaccttggt cccttggagg aatcctctca tgcttacaac
     3121 aaaaaaatgg ttaggcgcag ccttagctgc cctctgccta acagcgggcg tatctgaaac
     3181 agctttggca aaaccctaca aatcagcgga aatttttacc cccgcagcca acctctacgg
     3241 caaatacgta gtgcgcatgc gcgcagccaa agccagcggc gtaatctcca acttcttcct
     3301 ctggaaagac ggctcggaaa tgtccggcgt gttttgggaa gaagtggacg tagaagtctt
     3361 cggtaaaaac aacgccacct cctggcagag caatatcatc accggccaag gcacccgcac
     3421 aacctccgag caagtacaca acagcggctc gtcgtttggt gacggctaca acacctttac
     3481 cctcgagtgg agccccgaca aagtccgctg gttggtgaac aaccaagtga tccgcaccac
     3541 cgaaggcggc caggcccgca accttctaag cccttcgcag ttccgcttta acttctggcc
     3601 acccaacaac cctgagtggg ttggcagctg gaacgacagc gtgctgccgc aatacatgtt
     3661 cgtaaactgg attgaattcc accgctggaa cggctcaggc tttgaactgg cttggcgcga
     3721 tgatttcaac agctttgatt ccaaccgttg gggcaaagcc gactggacct tcaccgaaaa
     3781 ccgcgccgac ttttcaccca acaacgtggt ggtgaaaaac ggctacctgg tattggccat
     3841 gactcgcgaa ggccaagagg gctacaacgg cacgccccct gtcgataatg gcggctccaa
     3901 tagcagttct tccagcagtt catccagtag cagttcttct agctcatcca gcagcagctc
     3961 a
//
LOCUS       DAOQGS010000388         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12646_length_3954_cov_3.981534, whole genome shotgun sequence.
ACCESSION   DAOQGS010000388 DAOQGS010000000
VERSION     DAOQGS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12646_length_3954_cov_3.981534"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKZ52_13370"
     CDS             <1..1033
                     /locus_tag="PKZ52_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNG60808.1"
                     /translation="PDPDVIRMMGDKVAAIEAMKKAGVPTVPGSGGPLPEDADECHAI
                     AAKIGYPIIIKAAAGGGGRGMRVVHTEAALINSIHVTKGEAKAAFGDDTVYMEKYLQN
                     PRHVEVQILADGQGSAIHLFDRDCSLQRRHQKVLEEAPAPGIPDEVRKEVYAACTKAC
                     VDIGYKGAGTFEFLYEDGRFYFIEMNTRIQVEHPVTEMVTGIDLIKEQILVCSGQKLR
                     IKQKDVVLRGHAFECRINAEDPSTFMPSPGKITNFHAPGGLGIRVDSHVYSGYSVPPY
                     YDSMIAKVISFADDRPAALAKMRVALDELVISGIKTNTPLQQDLVRDAAFAQGGVNIH
                     YLEKKLQLK"
     gene            1088..1957
                     /gene="prmA"
                     /locus_tag="PKZ52_13375"
     CDS             1088..1957
                     /gene="prmA"
                     /locus_tag="PKZ52_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNG60809.1"
                     /translation="MPWLELRIDTDRDAAPRWEQALLKAGALSVTLQDNADQPIFEPA
                     LGETPLWQATRVTGLFDSEVDTARLERQMRLPTDSFWQWQLLEDKDWEREWMKDFHPI
                     PVAAGFWICPSWITPPEPNAVNLLLDPGLAFGTGTHPTTFMCLEWLSSQSLRGLTCID
                     YGCGSGILGIGALLLGASSVLGVDIDPQALIATQDNAQRNGLAPERFPVVFPKACPTT
                     AVDILLANILAGPLVELAPELTPRVKPGGLLCLSGILHSQAQAVVDAYSDAFDFAPWR
                     TRDEWVCLSATKR"
     gene            1977..3266
                     /locus_tag="PKZ52_13380"
     CDS             1977..3266
                     /locus_tag="PKZ52_13380"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HNG60810.1"
                     /translation="MADMITRCPQCATAFRITAAHLKLAQGSVRCGACFTIFNALENL
                     SERSTSNAPASNLADDFLISDHMGIEDESSETSAEASTKSSLFERVEQAKAQPEKSEE
                     ADESWAIALLSEEGLAEVHASDDEFLEEEPSFRPFSPGPSDKPIELSDDFLRATQKAQ
                     AAAQTQAREPQPPATAGAPQQAGADNAPADSEQDWATSLAEEHPELNADDLKDLQNAL
                     EQSRQEPVFASNRAEPAADFAPFSATAQVPGSRNAHLIERIELDPLEVTVRQRKGLSP
                     QFWWSLGGLCAMLALLLQVAIIKMPQWRYDTPLSGLYALACQKLACPAEARVDTSKID
                     TKSLLVHEHPSRPGAFAVDAVIVNKAPFVQPFPRLILSFRNLQNQLIYERIFLPAEYL
                     GGEMAGEKNMPIKQEIRIGLEILDPGRDAVSYNLTAD"
     gene            3508..>3954
                     /locus_tag="PKZ52_13385"
     CDS             3508..>3954
                     /locus_tag="PKZ52_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNG60811.1"
                     /translation="MLSIGPYSLSSQVLLAPMAGVSDLPMRKLCLAQGAGLAFGEMLI
                     SDTRHWGSRKSAARLQVEASTAPIAMQIAGSAPKQMAEAAKACVDAGAQIVDINMGCP
                     AKKVCNRAAGSALLRDETLVGQILDAVVAAVDTPVTLKIRTGWCPQS"
BASE COUNT          886 a         1210 c         1070 g          788 t
ORIGIN      
        1 cccagatccg gacgtgatcc gcatgatggg cgataaggtt gccgccattg aagccatgaa
       61 aaaagcgggt gtacccacgg tacccggctc cggcggccct ttgccggagg atgccgacga
      121 gtgccacgcc atcgccgcca aaattggcta cccgattatc atcaaagccg ccgctggtgg
      181 cggtggccgc ggcatgcgcg ttgtgcacac agaagcggcg ctgattaact ccatccacgt
      241 caccaaaggc gaggccaaag cagcctttgg cgatgacact gtttatatgg agaaatacct
      301 gcaaaacccc cgtcacgttg aggtgcaaat cctcgccgac ggccagggca gcgccattca
      361 cctattcgac cgggactgct ccttgcagcg gcgccatcaa aaagtactgg aagaagcccc
      421 ggctcccggc attcctgatg aggtgcgcaa agaagtctat gccgcctgta ctaaagcctg
      481 tgtggatata ggctacaagg gcgctggcac ctttgagttt ctctatgaag acggccgttt
      541 ttactttatc gagatgaaca cccgtattca ggttgagcac ccggtcaccg agatggttac
      601 cggcatcgac ctgatcaaag agcagatttt ggtgtgttcc ggccagaagc tgcgcatcaa
      661 acaaaaagac gttgtgctgc gcggccacgc atttgaatgc cgcatcaacg ccgaagaccc
      721 aagcaccttt atgcccagcc ccggcaaaat taccaatttc cacgcgccgg gcggcttggg
      781 tattcgggtg gattcccatg tctattccgg ctattccgtg cccccctact acgactccat
      841 gatcgccaag gtcatctcct ttgccgatga ccgcccagcg gccctcgcca aaatgcgtgt
      901 cgccttggac gaacttgtca tcagcggcat caaaaccaat acgccgctgc agcaggatct
      961 ggtgcgggat gcggcctttg ctcagggtgg cgtcaacatc cactaccttg agaaaaaact
     1021 gcaacttaaa taagcctcca ccctccccgc gccggtttta agccgcgcgg gcgaacccgg
     1081 acaccctatg ccttggttag aactgcgcat cgacaccgac cgcgacgccg ccccccgttg
     1141 ggagcaagct ctgctaaaag ccggggcctt gagcgtcacc ctgcaagaca acgccgacca
     1201 accgatcttt gaaccggccc taggtgagac ccccctgtgg caggccacgc gggtgaccgg
     1261 cctgttcgac tcggaggtgg ataccgccag gttagaacgc caaatgcgcc tgcccactga
     1321 tagcttctgg cagtggcagt tgctggagga taaagattgg gagcgggaat ggatgaaaga
     1381 cttccatcca attcctgtag ctgccggatt ttggatctgc cccagctgga taacaccccc
     1441 tgagccaaac gccgttaatc tactattgga cccaggcctg gccttcggca ccggcaccca
     1501 tcccactaca tttatgtgct tggaatggct cagtagccaa tcgttaaggg ggctcacctg
     1561 tatagactac ggctgcggct ccggcatttt gggcataggc gcactgctgc tgggggccag
     1621 ttccgtgctt ggggtggaca tagatcccca agctctaatc gccacccaag ataatgccca
     1681 gcgcaacggc ctggcccctg agcggttccc ggtggtcttc cccaaagcct gccccacaac
     1741 agcggtggat attctactgg ccaatattct ggccgggcca ttagttgaat tggcgccaga
     1801 gctcacgcca cgggtaaagc ctggcgggct attatgtctg tcgggtattt tacatagcca
     1861 ggcgcaagcg gtggtggatg cctacagcga cgcatttgat tttgccccct ggcgcacccg
     1921 cgatgagtgg gtatgcttaa gcgccactaa acgctaactt ttatctagga aattccatgg
     1981 ccgatatgat tacacgctgc ccgcaatgcg ccacagcgtt tcgtatcact gcggctcacc
     2041 taaaactcgc ccagggatcg gtgcgctgcg gcgcctgttt cactattttt aacgcgctgg
     2101 aaaacctctc cgaacgctcc acctcaaacg cccccgcctc caatcttgcg gatgatttct
     2161 taatcagcga ccacatgggg atcgaggatg agagcagcga gaccagcgct gaggccagca
     2221 ccaaatcctc tctttttgaa cgggttgaac aggccaaagc ccagccggaa aaatctgaag
     2281 aagccgacga gtcctgggcc atcgcactgc tgagcgaaga agggctggcc gaagtacacg
     2341 ccagcgacga tgaatttttg gaagaggaac ccagcttccg cccctttagc cccggcccca
     2401 gcgataagcc cattgaattg tccgatgact ttttaagggc gacccaaaaa gctcaggctg
     2461 cggcgcaaac ccaggcccgt gagcctcagc ccccagcaac tgccggcgcc ccgcaacagg
     2521 caggcgcgga caatgcaccc gccgacagcg agcaggattg ggccacctcc ctggccgagg
     2581 agcacccgga gcttaacgcg gatgatttaa aagacctgca aaatgccctg gagcaaagcc
     2641 gccaggaacc ggtgtttgcc agcaaccggg ccgagcccgc tgctgatttt gcccccttta
     2701 gcgctaccgc ccaggtgcct ggttcgcgca atgcgcatct aatcgagcgc attgaacttg
     2761 acccattgga agtcaccgtc cgccagcgca aaggcttgag ccctcagttt tggtggagcc
     2821 tcggcggctt gtgtgccatg cttgctctgc tgctacaggt ggcgattatc aaaatgcccc
     2881 agtggcgcta cgacacgccc ttaagcgggc tgtatgcact ggcctgccaa aagcttgcct
     2941 gcccggcaga agcccgcgta gacaccagta aaattgacac caaaagcctg ctggtacacg
     3001 agcaccccag ccgcccgggc gcttttgcgg tagatgccgt catagtcaat aaagcgccct
     3061 tcgtgcagcc ttttccgcgc ttaattttga gtttccgcaa tctgcaaaac caactgattt
     3121 acgagcgtat ctttctgccc gccgagtatc tgggggggga aatggccggg gagaaaaata
     3181 tgcccatcaa acaggaaatc cgcatcggcc tggaaattct cgaccctggc cgcgacgccg
     3241 tcagctataa cctcaccgcc gattagggcc tgaatggggc cgagcaagcc tgccgccaca
     3301 ggatcgccaa aattcgctcc aacctttgtc tgcaaacagt gaatccattc aaaaaattgt
     3361 aacaacgaat atttagctat tttttgagca atctgctcaa attgtgcgtt ccaaaaccac
     3421 gccagtaaag gtagaatgcg cgcctttttg gctatccatg agcttgttgg atgcgcctaa
     3481 tcgtcaactc cctgaggagc aaaccatttg ctaagcatcg gcccctactc actgagctcc
     3541 caagtgctgc tggcacccat ggcgggcgtc agtgacttac ccatgcgcaa gctctgctta
     3601 gcgcagggtg cgggcttggc gttcggggag atgctcatca gcgatacccg ccactggggc
     3661 agccgcaaaa gtgctgctcg cttgcaagtt gaagcctcca cggcgccgat tgccatgcaa
     3721 attgccggca gcgcccccaa acagatggcc gaggccgcca aggcctgtgt ggacgcgggc
     3781 gcacagattg tcgatatcaa tatgggctgc ccggcgaaaa aggtctgcaa ccgcgccgca
     3841 ggctcagccc tgttgcggga tgaaactcta gtaggtcaaa ttctcgatgc ggtggtggcc
     3901 gcagtggata caccggtcac cttaaaaatt cgcaccggct ggtgccccca gagc
//
LOCUS       DAOQGS010000389         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12675_length_3947_cov_3.802415, whole genome shotgun sequence.
ACCESSION   DAOQGS010000389 DAOQGS010000000
VERSION     DAOQGS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12675_length_3947_cov_3.802415"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /gene="nhaD"
                     /locus_tag="PKZ52_13390"
     CDS             complement(<1..1084)
                     /gene="nhaD"
                     /locus_tag="PKZ52_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNG60812.1"
                     /translation="MVAALIIVFILAYAAIAFEHPLNINKSASALVGAGLLWTIYALC
                     APDTHALSDELSHTLAATAQIVFFLMGAMTIVEVIDAHNGFQVVTQRIKTQSLARLMA
                     VVVAVTFVLSAILDNLTTTIVMVSLMRKLLSQREDRLLFAGMIVIAANAGGAWSPIGD
                     VTTTMLWIGGQITPLNIVLSLLLPSLVNALIPLALVVYKLRGRRVIAPLIEQADDHGT
                     SSLERNWVFFLGLSVLIAVPIFKAVTHLPPFIGVLFGLGIVWLAADILHKNKHAEQKS
                     HLTLVHALTRIDMASIVFFIGILFAVATLEHTHILSALAAWLDETMGRQDLIVVLIGL
                     ASAVVDNVPLVAASMGMYSLEQYPADG"
     gene            1199..1333
                     /locus_tag="PKZ52_13395"
     CDS             1199..1333
                     /locus_tag="PKZ52_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60813.1"
                     /translation="MVHSTSKTGLAPSSAGKYRELRDKYTQLRSQLGQIENTLQFTRH
                     "
     gene            2109..3566
                     /locus_tag="PKZ52_13400"
     CDS             2109..3566
                     /locus_tag="PKZ52_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60814.1"
                     /translation="MTQIVHPVPSPAGLKARQLAGVMLLASLLAACGADPAPSSSSSS
                     SSSSSSSSSSSSSSGRSSSSSSSSSSSNSSSSSSSSSSSGHSHTVDHCTDGFSPQASD
                     ATMANTIATFTQNNQTDVVVQPEVIKWMEDHAWQEAHFTWHEVRRCSSFGTRGGVINP
                     CTFPEMKPEQNECTGAQDGYEFLVMHRHMIQSLKQLWPKHASQFDGWKKFPAKSDYPD
                     VVQQYFKAWTSANTTNAALADNIDTMTKEEVLRRWPTEGDFGKWIQCSDSGLHGALHF
                     NGYPPNNQAHSIANQKRNLDGYLFWKLHGWIDNVWEKYRLKTGIAPNDAKLQKELVAQ
                     CREMDFWSSKIDPNSVKPDEPVVIAPEKGYFHETVRPALERAGCATCHGVGEDGNLRL
                     GYEISSADIVKRLVNVDSANAEAYRLVVPGDPEHSWLYLKASGGSKTASVVCRDGATG
                     CKQSMPPGGGAGLTQTELSALYKWIKDGAPVPTKL"
BASE COUNT         1082 a         1106 c          925 g          834 t
ORIGIN      
        1 aaccatccgc cggatattgc tccaaactgt acattcccat ggaggccgcc accaagggca
       61 cattatccac caccgcacta gcaaggccga ttaacactac aattaaatcc tgccgcccca
      121 tggtttcatc caaccaggcg gctaatgcac ttaaaatatg ggtgtgttca agggtagcta
      181 ccgcgaataa aataccgata aaaaatacaa tcgacgccat atcaatacgc gttaaggcgt
      241 gcaccaaggt cagatgggat ttttgttcgg cgtgtttatt tttatgcaat atatccgctg
      301 ccaaccacac tatccctaag ccaaataaaa cacctataaa tggcggcaaa tgggtaacag
      361 ctttaaaaat cggcactgca attaatacac ttaaccccaa aaaaaatacc caattgcgct
      421 caagggaact ggtgccatga tcatctgcct gctcaataag cggcgctatt acccggcgcc
      481 cgcgcagttt ataaaccacc agcgccagag gaattaaggc atttaccagg gacggcaaca
      541 gtaaacttaa gacaatattc aaaggcgtaa tttgcccgcc aatccacagc atagtagtgg
      601 tcacatcgcc gatgggcgac caggcgccac cggcattggc ggcaattaca atcatgccag
      661 caaacaataa tcgatcctcc cgctggctta ataatttgcg cattagcgac accatcacaa
      721 tggtggtggt gagattatcc aaaatagcgc tcaacacaaa cgttaccgcc acaaccaccg
      781 ccatcaagcg cgctaatgat tgcgttttaa tacgctgggt aactacttgg aaaccattat
      841 gggcgtcgat tacttcaaca atcgtcatag cgcccatcag gaaaaataca atctgcgctg
      901 tggcggcaag ggtatgggaa agctcgtcgc tcagggcgtg tgtatcaggt gcgcaaagcg
      961 cataaatagt ccacaacaac cccgccccca ccaaagccga tgctgattta ttaatattca
     1021 ggggatgttc aaatgcaatt gccgcgtagg ccagaataaa aacaattata agtgcagcaa
     1081 ccatgggaat accttttaac caaatcgtta cctaaattta tcagcacagc accgcagctt
     1141 ttatcagtta ttcagccggt accaacacac aataagcggc cggccatggt acatttttat
     1201 ggtgcacagc actagcaaaa ccggcttggc gcccagcagc gcaggcaaat acagagagct
     1261 gcgcgataaa tacacccagt tgcgctcgca attgggccag atagaaaaca ccctgcaatt
     1321 cacccggcac taattgccgc cgggttaata aaaaactcta atcgcgccca aaataaccac
     1381 gacaaaaaat tcgtggtgat tgttatgcac aagtgtttaa aaattggaag ctattgggca
     1441 caccatatta atttgccgct ttccaaacgg acggcaaatt ttggaggatt ttttccaagc
     1501 ggcatagtca attcgcggtg cagcagtggt aaatacagat tattgcagac cgtcaaaaag
     1561 agggaccttt ttgacgagcc cacacggatg tagtcacggc gcgtctgcac taatctgtat
     1621 ttaccaacgc ggagggacgt aaaacactcg ccggatggtt gttttagtag cccggacaga
     1681 gcgaagcgta attgagggtg cgcagcctaa tccgggagtg ctcaagaacc ccaataacag
     1741 cctgtgcatc caggctaccc aagcaattcc acttctaatc tgcgcaactc aattctttaa
     1801 cttaacaaca ttcccgcttt agccaggtag tcaaaaccac cctaaaggac tgtcagaaaa
     1861 atcatacttt taagcaagaa gtgctgcttt taacagattt aaatcccaga ctcggtatga
     1921 tcgcctggtc atacaaattc tcggaggctg aatattcgcg gccgccacag caaccgctaa
     1981 aggccagggc tgggcttgtg ttagcgcgcg ctggtatccc gtatggcatc tacccaaccc
     2041 taaacctgca attcggccaa acccctgggc cgcaatctgc ggtttcacta taaaaaaaag
     2101 taatggctat gactcaaatt gttcaccctg tgccctcccc ggcggggcta aaagcgcggc
     2161 agttggcagg agtgatgctc ctggctagcc tgttagcagc ctgtggtgca gatcctgcac
     2221 cctcgtccag ttcctccagc agttcatcta gctcctcaag ctcatctagc tcaagctcct
     2281 ccgggcgcag ttcaagttcc tccagttctt caagctcctc taactcctcc agttcgtcca
     2341 gctcatcaag ttcatccggc cacagccaca cggtggatca ctgcacggat ggtttctcgc
     2401 cccaggccag cgatgccacc atggccaaca ccatcgcgac cttcacacaa aacaatcaaa
     2461 ccgatgtggt cgtacagcct gaagttatta aatggatgga ggaccacgcc tggcaggaag
     2521 cccactttac ctggcacgaa gtgcgccgct gcagcagttt tggtacccgc ggcggagtta
     2581 ttaacccctg caccttcccg gaaatgaaac cggagcaaaa cgagtgtacc ggcgcccaag
     2641 acggctacga gttcctcgtc atgcaccgcc acatgattca atccctcaag cagttgtggc
     2701 ctaaacacgc ctcacaattt gacggctgga agaaattccc cgccaaatcc gattaccctg
     2761 acgtagtgca gcagtacttc aaagcctgga ccagcgccaa taccaccaac gctgccctgg
     2821 ccgataacat cgacaccatg accaaagagg aagtgttgcg gcgctggccc accgaggggg
     2881 actttggcaa atggattcag tgcagcgaca gcggcctgca cggcgcactg cactttaacg
     2941 gttacccacc gaacaaccag gcccacagta tcgccaacca aaagcgcaat ctggatgggt
     3001 atttgttctg gaaactacac ggctggatcg acaacgtctg ggaaaaatac cgcctcaaaa
     3061 ccggcatagc gcccaacgac gccaagctgc aaaaagaatt ggtagcccag tgccgggaaa
     3121 tggatttttg gtccagcaaa atcgacccca actcggttaa acctgacgag cctgtggtca
     3181 tcgcaccgga gaaaggctac ttccatgaaa ccgtgcgccc cgccctagag cgcgcaggct
     3241 gcgccacctg ccatggcgta ggggaagacg gcaacctgcg tttaggctat gaaatcagct
     3301 ccgccgacat agtcaaacgc ctggtgaatg tagactccgc caacgctgaa gcctaccgcc
     3361 tggtggtgcc gggggaccct gagcacagct ggctgtacct aaaagcctcc ggcggcagca
     3421 aaactgcatc agtggtgtgc cgcgatggcg caacaggctg caaacagtct atgccccctg
     3481 gcggcggtgc gggcctaacc caaactgagt tgagcgcgct gtataaatgg attaaagacg
     3541 gcgctccggt tcccaccaaa ctttaacccg ataatcttca gttaaacgcg tagcggcggt
     3601 ttaactgaag cagctgggtt tagctaaaga atgcaccgct acaaaaaaac cgctcgctgg
     3661 gttagctctg cgagcggttt tttatgccgg aaattttgtc ggcggcttca ccgcggttgt
     3721 agcctaaatg tcattaggtt acggtttgcc tgggtttttt gtagcctggg tggagcgcag
     3781 cgcaatctgg gggcgcgcaa aaaacccgga ttgcgctgcg cttcacccag gctacctagg
     3841 taattcctct caaaatagac aaaccacaat ccttaacttc acgatcctta acttaatcac
     3901 agggtcgtgg ccccagcgag cgcgctgcag gggttaactg cggtttt
//
LOCUS       DAOQGS010000390         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12684_length_3945_cov_4.716195, whole genome shotgun sequence.
ACCESSION   DAOQGS010000390 DAOQGS010000000
VERSION     DAOQGS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12684_length_3945_cov_4.716195"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKZ52_13405"
     CDS             complement(<1..402)
                     /locus_tag="PKZ52_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG60815.1"
                     /translation="MSYQVLARKWRPKIFREMVGQEHVLRALINALDHNRLHHAYLFT
                     GTRGVGKTSIARILAKCLNCERGVTSEPCGVCSACKEIAEGRFVDLIEVDAASRTKVE
                     DTRELLDNVQYLPSRGRYKVYLIDEVHMLSSS"
     gene            complement(534..630)
                     /gene="ffs"
                     /locus_tag="PKZ52_13410"
     ncRNA           complement(534..630)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKZ52_13410"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(765..1943)
                     /locus_tag="PKZ52_13415"
     CDS             complement(765..1943)
                     /locus_tag="PKZ52_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNG60816.1"
                     /translation="MFKVKTYNKISEKGLSRFAPGPYQLGADIADADAYILRSHKLHG
                     EPVPASLLAVARAGAGVNNVPVADYSKQGIVVFNSPGANANAVKELVLAGMLLSSRDI
                     LGGKAYADSLTHMTDAAEMSKLLEAEKARFAGNELMGKTLGVVGLGAIGSMVAEMALA
                     LGMDVVGYDPALSVEAAWRLPSQVRRMENLNSLLAASDYVTLHVPAIEATKHMISATS
                     IKAMKPTAALLNFAREAIVDSAAVSAALESGGLRQYICDFPEPGMLNRKDVIALPHIG
                     ASTEEAEENCAIMAANQLRDFLENGNITNSVNFPAIAMSRVPGRCRLTFSNNNVSGVL
                     GNVLSIFTEHKVNVIDMTNKSRGELAYNILDLEQVPGSDVLAAIANVAEVIRVRLIPA
                     "
     gene            complement(2292..3410)
                     /locus_tag="PKZ52_13420"
     CDS             complement(2292..3410)
                     /locus_tag="PKZ52_13420"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNG60817.1"
                     /translation="MQPSVKPANPNFSSGPCSKRPGYSVNQLVIDTLGRSHRSALGKK
                     ALEAACVETAELLGLPAGYRVGVVPGSDTGAVEMALWSLLGARPVDVLAWESFGSGWV
                     SDIMQELKIKDVRKFVADYGTLPDLSAVNFHHDVVFTWNGTTSGVKVPNGDWIAADRQ
                     GLTICDATSAVFAMDMPWEKLDVVTFSWQKVLGGEGAHGMLILSPRAVERLESYTPPW
                     PMPKLFRMTKGGKLVEGIFKGDTINTPSMLCVADYLDALGWVRSIGGLSAAIARSNAN
                     LAVLEDFVAKTPWIHFLAKNQAERSNTSVCLTLDVSEAQVKTIIKLLEQEKVAFDVGA
                     YRDAPPGLRIWCGATVETADLKALLPWVEWAYQQATAA"
     gene            complement(3711..>3945)
                     /locus_tag="PKZ52_13425"
     CDS             complement(3711..>3945)
                     /locus_tag="PKZ52_13425"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG60818.1"
                     /translation="LLAAEPESTALAHQLALQYSQHGYTSEALELLYSLLKKNLQAEE
                     GAVKRTYTDIIATLPKGDPLAVSYQRKLYALLY"
BASE COUNT          880 a         1089 c         1124 g          852 t
ORIGIN      
        1 gctgctggag agcatgtgta cttcgtcgat caggtacacc ttgtagcggc cccggctggg
       61 taggtactgc acgttatcga gtagttcccg ggtgtcctcc accttggtgc gcgaggccgc
      121 gtccacctcg atcaaatcca caaagcgccc ttcggcgatt tctttgcagg ccgaacacac
      181 gccgcagggc tcagaggtaa ccccgcgctc gcagttcaaa cacttggcca ggatacgggc
      241 gatactggtc ttacccacac cgcgggtgcc ggtaaacaaa taggcgtggt gcaagcggtt
      301 gtgatccagg gcgttaatca gcgcccgcag cacatgctcc tgccccacca tttcgcgaaa
      361 tattttcggc cgccacttac gggccagtac ctgataactc acagtcgtct cacaggctca
      421 aaagggggcg gattataacg caactgatcg cgctaaaacg caatgcgcac agactaattg
      481 gcggctttgc gacggggaga aaaaggggga aatacgagga gaattggagg ggcccttacc
      541 agccacatcc cggcacacaa ctgaaccgct accgttgctc ccttccgggc ctggcggggt
      601 ttacggcaag tcattgcggg aggaccgata aggaccacca taacaaaccg cagcagcaag
      661 cgctgctggg aggcggcatt atcctgattt tactggggat tgcaaggatt agatggaagg
      721 caggggggca tggccagggg tgagcgctat gccccgccat gggtttaggc cgggattaac
      781 ctaacccgaa tcacctcagc cacattggca atggcagcca acacatcact gccgggcacc
      841 tgttctaaat ccaggatgtt gtaggccagc tcgccacggc ttttgttggt catgtcgatc
      901 acattcacct tgtgctcggt gaagatggac agcacattgc ccaacacgcc agagacattg
      961 ttgttgctga aggtgagacg gcagcggccg ggtacgcggc tcatggcaat agccgggaag
     1021 ttaaccgaat tggtgatatt gccgttctct aaaaagtccc gcagttggtt ggccgccatg
     1081 atggcgcagt tctcttcggc ctcttcggtg ctggcgccta tgtggggcag cgctatgaca
     1141 tctttgcggt tgagcatgcc aggctcgggg aagtcgcaaa tgtattggcg caggccacca
     1201 ctttccagcg cggcacttac ggcagcggaa tccacaatgg cttcgcgggc gaaattcagc
     1261 agcgctgccg tgggcttcat ggctttgata ctggtggcgc tgatcatgtg tttggtggct
     1321 tcaatagcag gcacatgtag cgtgacatag tccgaggcgg ccaacaggct gttgaggttt
     1381 tccatgcggc gcacttggct gggcaggcgc caggcagctt ccaccgatag ggccgggtcg
     1441 tagcccacga catccatacc caaggccaga gccatttccg ccaccatgga accaatggcg
     1501 cccaggccca caaccccgag ggttttgccc atcagttcgt taccggcaaa acgggctttc
     1561 tctgcctcca acaatttgga catctcagcc gcgtcggtca tatgggtaag gctatcggca
     1621 taggctttac cgccaagaat atcccgcgag ctcaacaaca ttccggccag taccagttct
     1681 ttaacggcat tggcattggc accgggggag ttaaatacca ctatgccctg cttgctgtaa
     1741 tcggccaccg gcacattgtt cacaccggca ccggcgcggg ccaccgccaa caggctggct
     1801 ggcaccggct cgccgtgcag cttgtggctg cgcagaatat aggcgtctgc atcggcaatg
     1861 tcggcgccca attggtaggg gccgggcgca aagcggctga ggcctttttc agaaattttg
     1921 ttataggttt ttactttgaa catgggtagc cccgtgagag tctcaatccg tccactgaaa
     1981 gccagctgtc aaaatgtcga aacaattgac aagcggcccc agccattaaa cataacaagc
     2041 tgatttagtt ataaaaatat aaatcggctt tatatgtgct aaacccggtc accttactaa
     2101 agattgctgg tctggaattt ttagctggta aaaccttgtt tcttcgattt ttgccccaga
     2161 gatatcgggg cttttttttg cctggcgttt taccacaagc cttaaggggc aaagtcctta
     2221 aaactcttat ccgccacccc agacaaaaaa gcgccgcaat acagcggcgc catcactgcc
     2281 tctaactttt gttaagctgc ggtagcctgt tggtaggccc actcaaccca gggcagcagc
     2341 gcttttaagt cggctgtttc caccgtagcg ccgcaccaaa tgcgcaggcc cggaggtgca
     2401 tcccggtagg cgcccacgtc aaaggcgacc ttttcctgct ccaataactt aatgatggtt
     2461 tttacctggg cttcgctgac atccaaggtc aaacatacgc tggtgttgga gcgttcggct
     2521 tggttcttgg ctaaaaagtg aatccaaggt gttttggcca cgaagtcttc caacacggca
     2581 aggttggcgt tggagcgggc gatggcggcg gagagaccgc cgatagagcg cacccagccg
     2641 agggcgtcaa gataatcagc cacacacagc atagagggcg tgttgatggt gtcgccttta
     2701 aaaatccctt caactaattt gccgcccttg gtcatgcgaa atagtttcgg catgggccaa
     2761 ggtggggtat aggactccaa gcgctcaacg gcgcgtgggc ttaggatcaa cataccgtga
     2821 gccccttccc cgccgagcac tttctgccag gagaaggtca ccacatcgag tttctcccaa
     2881 ggcatatcca tagcaaatac cgctgaggtg gcatcacaaa tggtaaggcc ttggcggtcg
     2941 gcggcgatcc agtcgccgtt gggaacctta acgccggagg tggtgccatt ccaggtgaat
     3001 accacgtcgt ggtgaaagtt aacggcggat aagtccggca atgtgccgta atcggcgaca
     3061 aacttgcgca cgtctttgat tttaagttcc tgcatgatgt cgctcaccca gccggaaccg
     3121 aaggattccc aggccagcac atccaccggg cgggcgccga gcaaagacca cagggccatc
     3181 tccaccgcgc ctgtgtcgga gccgggcaca acgcctacgc ggtaaccggc gggcaggccg
     3241 agcagctcag cggtttccac acaggctgct tccagggctt ttttacccag ggctgagcgg
     3301 tgtgagcggc ctagggtatc aatcaccaat tggttaaccg aataaccagg gcgcttgctg
     3361 caggggccgg aggaaaaatt gggattggcg ggttttacac ttggctgcat ggatcttctc
     3421 aacttgtaaa aataaaacgg atagggccag ttttaggcgc tggccaatgc ttgtagcctg
     3481 cggcgctctg atggatttaa cgcgctggcg atcctgggtg cgaaaaaggc cggacattat
     3541 gccagcaatg ccccacctgg caagcggaca ttgggtcaaa tcttggccgg ctagatgtac
     3601 gtcgtgaagc aaggcctcgg ctcagactgg ccttaagctg gcagcggaca atttgggttg
     3661 atgctcagtc cgcaaaaact catgtcccgg gtcgcccgca ctcagaagaa ctaatacaac
     3721 agcgcgtaga gtttgcgctg gtaactcacc gccaaggggt cgcccttggg cagggtggcg
     3781 ataatatcgg tataggtgcg tttaaccgcc ccctcttctg cctgcagatt ttttttcagc
     3841 agtgaatata aaagctccag ggcctcactg gtgtacccgt gctgggagta ttgcagggcc
     3901 aattgatggg ccagtgccgt gctttcaggc tcagccgcca gcaag
//
LOCUS       DAOQGS010000391         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12817_length_3919_cov_4.864130, whole genome shotgun sequence.
ACCESSION   DAOQGS010000391 DAOQGS010000000
VERSION     DAOQGS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12817_length_3919_cov_4.864130"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKZ52_13430"
     CDS             complement(<1..485)
                     /locus_tag="PKZ52_13430"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNG60819.1"
                     /translation="MSDTLRSRSSGFTLVELLVAISIAAILLAVAIPSFQSLIKRNAV
                     ENLQTRLAAALTAARSEAASRNVVTTLCSSDTGTGCVANKWSSGWIVFIDLNADGIKD
                     NNEEILQTFQNKNGYFINFKNEANNAINSLSFTSQGFVRNDTRAYAAFCDPDKISNYT
                     QG"
     gene            complement(1025..>3919)
                     /gene="metH"
                     /locus_tag="PKZ52_13435"
     CDS             complement(1025..>3919)
                     /gene="metH"
                     /locus_tag="PKZ52_13435"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNG60820.1"
                     /translation="SCYVSAHPNAGLPNEFGEYDQSPAEMALIIEEFARSGFVNIIGG
                     CCGTTPAHIAAIAKTVAPIPARKRPEIAPACRLSGLEPFNIFANSLFVNVGERCNVTG
                     SAKFKRLIIEENYTEALSVALEQVENGAQVIDINMDEGMLDAEKAMVRFLNLIAGEPD
                     IARVPIMVDSSKWHVIEAGLKCIQGKPIVNSISLKEGEAEFLEKAALCAQYGAAIVVM
                     AFDENGQADTKARKIEICKRSYDLLTQKIHFPPEDIIFDPNIFAVATGIDEHNNYAVD
                     FIEATAWIRKNLPHAGVSGGVSNVSFSFRGNNPVREAIHSVFLYHAIKAGMNMGIVNA
                     GQLAIYDDLPEILRNRVEDVISNRNSQATEALLEIAEQFKDGGGDHTKQKEDLAWRQL
                     PVAQRLAHALVKGITAHIEEDTEAARREAKRPLDVIEGPLMNGMNIVGDLFGEGKMFL
                     PQVVKSARVMKQAVAYLQPFIEQEKDEKSRPNGKILMATVKGDVHDIGKNIVGVVLQC
                     NNFEVVDLGVMVPCEKIVQTAVAENCDIIGLSGLITPSLDEMVTIAKEMQAQGINKPL
                     MIGGATTSKAHTAVKIDPQFSLNQVVYVADASRAVGVASQLLSDELRPAFVASVKDDY
                     EKVRARRANRHNAVKLVPYEQALARSFAADWNTYTPPKPSFLGVKALNNYPLTELIDF
                     IDWTPFFLSWELAGKFPDILTDSVVGEAATHLYQDAKDCLQKIIANNWLQANAVIGFW
                     PATSDGDDIELTDTEGAPIAQLHHIRQQQEKNSTDNTLYSLADFIAPKSSGKHDYLGG
                     FVVTAGLGVDEIAREFERKGDDYTAIMIKALADRLAEAFAEHLHQRVRREFWGYAPAE
                     ALGNEALIKEQYQGIRPAPGYPACPDHTEKATLFKLLNATENTGVSLTEHFAMYPTAA
                     VSGWYFAHPQAKYFGVGKLGEDQIISLAKRKKIPKAQMEKWLQSHLDYLD"
BASE COUNT          917 a          918 c         1001 g         1083 t
ORIGIN      
        1 ccctgggtgt agttggagat tttatctggg tcgcagaatg ctgcgtaggc gcgggtatcg
       61 ttgcggacaa aaccttggct ggtgaaactc agtgaattga tggcgttatt agcttcattt
      121 ttaaagttaa taaagtagcc atttttattt tgaaatgtct gcagtatctc ttcgttatta
      181 tctttaatcc cgtcggcatt gagatcaata aaaacaatcc agccggatga ccatttattt
      241 gccacgcaac ctgtacctgt gtcgctgctg catagggtgg tgaccacgtt gcgggaggct
      301 gcttctgagc gggcggcagt taaagcagcg gcgaggcggg tttggaggtt ttcgacggcg
      361 ttgcgtttga tgaggctttg gaaggagggg atggctacgg cgaggaggat agcagcgatg
      421 ctaatcgcca ccagcaattc aacaagggta aagccgctgg atctggatct taaggtatcg
      481 ctcattcgag ttgccttgat agagagtgtc acatttgaga cgcgttacat ccgcatttag
      541 ccccaataga actttgtatt tagattaaac catggcttta ccatagagca tctgcccacg
      601 tacagtcacc ctgccttgga tgggcggcaa gagagtaaga ccaatggcag gtcgatttcg
      661 ctgctattga ttgggctaaa ttgagctttg aataagcgca aatcacgtca aaaatataat
      721 gggtagtcag tcacaaatca cgaatcgcaa gcgtcgagta ctcctataac ccgaggttgt
      781 tctagccaat tcacaaatct agctttttag gcgccgcttg ttgaatctgg atctggggat
      841 attgagcaaa ctacgcgggc aaacccctct tgctcgccta gggcaggatt ggtggggtgt
      901 gctggtatgt ctgagtaata ggtgcgaatt aagacttaat catcgcacct tactcagagt
      961 ttcttagagt ttcgtccttt gctccttgtg tgtgcgatga ggatttggct tattagggaa
     1021 agacctaatc caagtaatcc aggtgcgatt gcaaccactt ttccatctgc gctttaggaa
     1081 tttttttgcg tttggcgagg gaaattattt ggtcttctcc cagcttgccg acgccaaaat
     1141 acttggcctg aggatgcgca aaataccagc cggaaacggc cgcagttggg tacatggcaa
     1201 agtgctcagt gagcgatacg ccggtatttt ccgttgcatt gagcaattta aatagggttg
     1261 ctttttccgt atggtctggg caggccgggt agccgggtgc cgggcgaatg ccttggtact
     1321 gctctttgat taaggcctca ttacccagcg cctctgccgg tgcgtaaccc cagaattctc
     1381 tgcgcactcg ctggtgtaaa tgttcagcaa aagcttcggc cagacgatct gctagggctt
     1441 taatcatgat ggccgtgtag tcatcccctt tgcgctcgaa ttcccttgca atttcatcca
     1501 ccccaaggcc tgctgttact acaaaaccgc ctaggtaatc gtgtttgcct gaggatttag
     1561 gcgcaataaa atctgcaagg ctatacagcg tattgtctgt tgagtttttt tcttgttgtt
     1621 ggcggatatg gtgtaattga gcgattggcg caccctcggt atcggtgagc tcaatatcat
     1681 cgccgtcgct agtggcaggc caaaagccga ttaccgcatt cgcctgcaac cagttattgg
     1741 cgataatttt ctgcaaacag tcttttgcat cttgatacaa atgggtagct gcttcgccca
     1801 caaccgagtc ggttaaaata tcggggaatt taccggccag ctcccaactt aagaaaaagg
     1861 gtgtccagtc gataaaatca attaattctg tcagcggata attattgagc gccttcacgc
     1921 ccaaaaacga cggtttaggt ggtgtgtagg tattccagtc ggctgcaaat gaacgcgcaa
     1981 gggcttgttc ataggggact aatttaaccg cgttgtgccg attggcgcgg cgggcgcgca
     2041 ctttttcata atcgtctttt acgcttgcca caaaggccgg gcgcagttcg tctgataaca
     2101 attggctggc aacgcctacg gcgcgggacg catcggcaac atataccact tggttgaggg
     2161 aaaattgcgg gtcaattttc accgccgtgt gtgcttttga agtggtggcg ccaccgatca
     2221 tcaagggttt attaataccc tgtgcttgca tttctttggc aatggtaacc atttcatcta
     2281 aggatggtgt aatcaaacca gataaaccaa tgatatcgca gttttcagca acggctgttt
     2341 gcactatttt ttcgcagggc accataacgc ctaagtccac tacttcaaaa ttattgcatt
     2401 gcagtaccac gcctacaata tttttgccaa tatcgtgcac gtcacctttc actgtggcca
     2461 ttaaaatttt gccattgggg cgggattttt cgtctttttc ctgctcgata aatggctgca
     2521 gataggccac cgcttgtttc atcacccggg cagatttaac cacttggggc aaaaacattt
     2581 ttccttcgcc aaataaatca cccactatat tcatgccgtt catcagcggc ccttcgatga
     2641 catcaagggg gcgcttggcc tctcggcggg ccgcttcggt gtcctcttca atatgggcgg
     2701 taatgccttt aaccaatgcg tgggcaagcc gctgggcgac aggtaattgc cgccaggcta
     2761 ggtcttcttt ttgtttcgtg tggtcgccgc cgccgtcttt aaattgttcg gcaatttcta
     2821 acagagcttc agttgcttgg ctgttgcggt tggaaataac atcttctact cggttgcgca
     2881 agatctctgg caaatcatca taaatagcca attgccctgc gttgacgatc cccatattca
     2941 tacccgcttt tattgcgtgg tagagaaata ccgagtgaat ggcctcgcgc acgggattgt
     3001 tgccgcgaaa ggaaaacgac acgttgctca cgccaccgga gacaccggcg tggggcagat
     3061 ttttgcgaat ccaggcggtg gcctcaataa aatccacggc ataattattg tgttcgtcga
     3121 taccggtggc gacagcaaaa atattggggt caaaaataat atcttccggc ggaaaatgta
     3181 ttttttgtgt gagcagatca taactgcgct tacaaatctc aattttgcgc gctttggtat
     3241 cggcctggcc gttttcgtca aaggccatga ccacaatggc ggcgccgtat tgggcacata
     3301 aggcggcctt ttctaaaaac tccgcttcac cttctttaag ggaaattgag ttgactatgg
     3361 gtttgccctg aatgcatttt aaaccggcct caattacgtg ccatttggag gaatccacca
     3421 taatgggtac gcgggctata tccggctcac ccgcgattag gtttaaaaac cgcaccatgg
     3481 ctttttcggc atctaacatg ccctcatcca tattgatgtc aattacctgg gcgccatttt
     3541 ctacctgctc taatgctacg cttagggcct cggtgtaatt ctcttcaata atgaggcgtt
     3601 taaatttggc ggagccggtg acattgcagc gctcgcccac attcacaaat aatgaattgg
     3661 caaaaatatt aaacggctca aggccggata aacggcaggc aggggctatt tctgggcgtt
     3721 tgcgcgctgg aataggtgcg acggttttgg cgatggccgc gatgtgcgca ggagtggtgc
     3781 cgcagcagcc gccgatgata ttcacaaaac cgctgcgggc aaattcctcg atgattaatg
     3841 ccatttctgc cgggctttga tcgtattcgc caaattcatt aggcaagccc gcgttggggt
     3901 gggcggagac ataacagct
//
LOCUS       DAOQGS010000392         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12877_length_3908_cov_4.222424, whole genome shotgun sequence.
ACCESSION   DAOQGS010000392 DAOQGS010000000
VERSION     DAOQGS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12877_length_3908_cov_4.222424"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKZ52_13440"
     CDS             <1..421
                     /locus_tag="PKZ52_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNG60821.1"
                     /translation="RLWIAPPTKMDQHQLMEEGYYSIYGVKGARTEMPGCSLCMGNQA
                     RVATNSTVVSTSTRNFPNRLGEGADVYLASAELAAIAAVMGKLPTPEEYLSYASKINS
                     MGADIYRYLNFNEIASYQAAADEGKRIAAVEITTLAI"
     gene            complement(1171..2274)
                     /locus_tag="PKZ52_13445"
     CDS             complement(1171..2274)
                     /locus_tag="PKZ52_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNG60822.1"
                     /translation="MNAKLEMRTFLWMLLAISMGFLWMIQPFFAPIFWACALAIIYYP
                     WHIRLLARMPQRPNTAAMISLSICVLVIILPVVFIVAQVVSQAADAYSHIEKGAFDPG
                     QYLERVNSLFPQAQPLLKKLGLDINNLKKTAMDAAMVGGRFAAEHALNIGQNAAGLLL
                     DFSLMLYLSFFMLRDGNQLLELLVRALPLGDARERQLFALFGEVTRATVKGNLVVALV
                     QGALGGLILFILGMPGVLLLAVLMAFASLIPAVGPALVWAPIALYWVATGQYWQAGVL
                     TGFGVLVIGMVDNVLRPILVGRDTKMPDYIVLLSTLGGIALFGINGVVVGPLVAALFM
                     AFWGIFMAEIHVSGDALIAPANTSVTDEEPHNN"
     gene            2349..2831
                     /locus_tag="PKZ52_13450"
     CDS             2349..2831
                     /locus_tag="PKZ52_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNG60823.1"
                     /translation="MPSFDIVSEINAEEVRNAVDIASRELDTRFDFRGVEASFKWTKD
                     ETTLSAEADFQLQQMVDILRNKLVKRNVDTSAMEIGKAVHSGKTFSQKITFKQGVETE
                     VAKKIVKLIKDSKIKVQAAIQGDQVRVTGKSRDDLQETMALVRTADLGQPFQFTNFRD
                     "
     gene            complement(3094..>3908)
                     /gene="purU"
                     /locus_tag="PKZ52_13455"
     CDS             complement(3094..>3908)
                     /gene="purU"
                     /locus_tag="PKZ52_13455"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNG60824.1"
                     /translation="GIVASVASLFSLQGFNIRESSQFEDVYTSRFFMRTVLESVEGPK
                     NIAALRATFSAIADRYGMTWELHDGSYRPKLLIAVSQWGHCLNHLLNSAKQGSLPVDI
                     VGVVSNHNELRDLTQWYGVPYHYLPVTPDTKAEQEAQILNLMHASGADFLALARYMQI
                     LSNDLCAALSGRAINIHHSFLPGFKGAKPYHQAFDRGVKLIGATAHYVTADLDEGPII
                     EQEVERVTHALTPEQLVEIGRDIEAVVLARAVRWHAEHRVILNGQKTVVFAR"
BASE COUNT         1097 a          967 c          924 g          920 t
ORIGIN      
        1 ccgcctgtgg attgccccac ccaccaagat ggatcaacac caattaatgg aagagggtta
       61 ttacagcatt tacggcgtta agggtgcccg caccgaaatg cccggctgtt cgctgtgtat
      121 gggtaaccag gcgcgggtag cgactaattc aaccgtagtc tccacctcta ctcgcaactt
      181 ccccaaccgt ttgggtgaag gcgccgatgt atatttggcc tcggctgagt tggcggccat
      241 tgccgcggta atgggcaaac tgcctacgcc ggaagaatat ttaagttatg ccagcaaaat
      301 aaatagcatg ggggcggata tttaccgcta cttaaacttt aatgaaattg ccagttatca
      361 agcggcggcg gatgaaggca aacgcattgc ggcggtggaa attacaacgt tagcaattta
      421 accgctaatt cagaactaga agcccgcctt agatggcggg ctctagttct ggacgagaaa
      481 atcaaacaat atctacaaaa ggagctcgcg atgagaaccg ccacattatt cttgattgtt
      541 ttttcttccg ttgatgcatg gtctgctgct gaatgcacag aaaaggtaac cagagttata
      601 aatcacaaga acaatgggat ttatttccag acagataaaa cttgctcgag caactggtgc
      661 caaattgctt ggggtacaga agccgccaac aacaggggat ttagcgttct gttaaccgca
      721 aaaactacag acaagacagt aactatttac tggccagaat tgagtagttg cgatgcctta
      781 aaccccgttt acaaatcacc cgagtatatc atcctgtaaa aaaacgagtt gatagcaaca
      841 aggagactgc aatcaactcg ccacttcacc ccccccaata tatcgccacc agcccatttc
      901 caacactcaa gctaaagttg actggacaga tgttttacat gcgacgtcgg cgcataaatt
      961 atatacttgg cagccttgtc acattcatct attgattctg tattcactta gcaatatgaa
     1021 ttaacccatg ctgcaggtat tcgcagaacg agcatccata acaattatat tttccctggc
     1081 aagcaggccc tactgcaatt cccaactgtc aatttcaaaa tttaatttat gctcataaaa
     1141 ggcgatttgt gcatccatat tttaatccta ttaattattg tgcggctctt catcagtaac
     1201 agaagtattg gcaggggcaa ttaacgcatc cccactgaca tgaatctccg ccataaaaat
     1261 gccccaaaac gccataaata aggccgccac caatggcccc accactacgc cattaatacc
     1321 gaatagggct atgcctccca gcgtggacaa taacactatg taatcgggca ttttggtatc
     1381 gcgccccacc agtattggcc gcaatacatt atcaaccata cctatgacca ataccccaaa
     1441 accggtcaac accccggctt gccaatactg cccagtggcc acccaataca aggcaattgg
     1501 cgcccacacc aaagcagggc cgacagcggg aatcagtgag gcaaatgcca ttaataccgc
     1561 caacaacaaa acccccggca tacccaaaat aaataaaatc aagccgccta aggcgccctg
     1621 cactaatgcc actaccaaat tgccttttac ggttgcacgg gttacctcgc caaacagcgc
     1681 aaataattgt cgctcccggg cgtcacctaa gggcagtgcg cgcactaata attccaataa
     1741 ttggttgcca tcgcgcagca taaaaaagct taaatacagc attaaggaga aatccaataa
     1801 taggcctgca gcgttctggc ctatatttaa agcgtgttca gcggcaaacc taccacccac
     1861 catagcggcg tccatggcgg tttttttcaa attattaata tccaggccga gtttttttaa
     1921 tagcggttgc gcctggggga ataaggaatt aacgcgctct aaatattggc ccgggtcaaa
     1981 agcacctttt tcaatgtggc tgtaggcatc ggccgcttgg cttactacct gcgccacaat
     2041 aaacaccacg ggcagaataa tcaccagcac acaaatactc aggctgatca tggcggcggt
     2101 attgggcctt tggggcatgc gtgccagcag gcgtatatgc caggggtaat aaatgatggc
     2161 cagcgcacag gcccaaaaga tgggggcaaa aaacggttgg atcatccata agaagcccat
     2221 ggagatggcg aggagcatcc aaagaaaggt gcgcatttca agtttggcat tcataggcgt
     2281 gggccttggg ttgggtgttg gggtgggctg atgtaaaatg ccgttttttg tcttgtgggg
     2341 aatttaccat gccttcgttt gatattgtct cggaaattaa tgctgaagaa gtgcgcaacg
     2401 ccgtggatat tgccagccgt gaactggata cccggtttga ctttcgcggt gtggaagcga
     2461 gttttaaatg gaccaaggat gaaaccaccc tcagtgccga agcggatttt cagctgcaac
     2521 aaatggtgga tattctgcgc aacaaactgg ttaagcgcaa tgtggacacc agcgccatgg
     2581 aaattggcaa ggcggtgcat tcgggtaaaa ccttcagcca gaaaattacc tttaaacagg
     2641 gcgtggaaac ggaagtggcg aagaaaatcg tcaagctgat taaagacagc aaaattaaag
     2701 tgcaggcagc tattcagggc gaccaggtgc gggtgaccgg caaaagccgc gatgatttgc
     2761 aggaaaccat ggcgctggtg cgcacagcgg atttaggcca gccgtttcaa tttactaatt
     2821 tccgagattg atccacggag gtcataaaat tgggtttggc aatttttagg ttgtttaacg
     2881 ctgtaaatta tcacttcctt tgcagcatgt ttattaattt aagacccatt cgtaggggca
     2941 acccttgtgg ttgcccctta tttacctcga ctatcaatca ggatttgcaa tgaatggggc
     3001 acccgcaagg ggcgccccta cgatgccgcc taacccaata agattggggc aaatttagcg
     3061 cgctgtcgtc attcaattta ccgagaattc tttttagcgc gcaaacacta cggttttctg
     3121 cccatttaaa atcacccggt gctcggcatg ccaacgcacc gcacgggcca atactaccgc
     3181 ctcaatatcc cgcccaattt ccaccaattg ctccggagta agtgcatggg ttacccgctc
     3241 tacctcctgc tcaataatcg gcccttcgtc taaatcggcg gttacataat gggcggtggc
     3301 gccaattaat ttaaccccgc gatcaaacgc ctgatggtag ggtttggcgc ctttaaaacc
     3361 gggcaaaaag gagtggtgaa tattaatggc acggccggac agcgccgcgc ataaatcatt
     3421 ggagagtatt tgcatataac gggcgagggc taaaaaatct gcgccacttg catgcattaa
     3481 atttaagatc tgcgcctctt gctcagcttt agtatcgggg gttacgggta aataatgata
     3541 gggcacgccg taccactggg ttaaatcccg cagctcgtta tggttggaaa ccacacccac
     3601 tatatccacc ggcagggagc cctgtttggc actatttaat aaatgattta agcagtggcc
     3661 ccattgggaa acggcaatta ataatttcgg ccggtagctg ccatcatgta attcccaggt
     3721 catgccgtag cggtcggcga tggcgctgaa ggtggcgcgc agggcggcga tattttttgg
     3781 cccctcaacg gattccagca ccgtgcgcat aaaaaaacgc gaggtgtaca catcttcaaa
     3841 ttgcgaggat tcgcgaatat taaagccctg cagggaaaat aaactggcca ccgaggccac
     3901 aatgccgg
//
LOCUS       DAOQGS010000393         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12887_length_3907_cov_3.812565, whole genome shotgun sequence.
ACCESSION   DAOQGS010000393 DAOQGS010000000
VERSION     DAOQGS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12887_length_3907_cov_3.812565"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKZ52_13460"
     CDS             complement(<1..755)
                     /locus_tag="PKZ52_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60825.1"
                     /translation="MVANALALGADEVMHVRHYDQGYTPSIKAYQKDLLQLMLENTVP
                     EFGAYSLDLFSVPLSTNRSKLTLQQGDKVNVLFASEWQGLGVSDEQVLSLELPIYYGL
                     HGLRQLIVRASDQDVFAKINSLQQFKSLSCGQGTRWLDVNIYRSNGIVVNEGQSFINL
                     MPMLQKKRFDYLPLSVLETDRVFSERAPELKDLIVLDDLMIFYPLPSFLFFPASEEHL
                     LERLRAGFAQLEQNGELLPLFERHFNSVPRILSG"
     gene            1491..3041
                     /locus_tag="PKZ52_13465"
     CDS             1491..3041
                     /locus_tag="PKZ52_13465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60826.1"
                     /translation="MGKKNFFQRLMRENHLQRELIIWVSLAVVLPGALMAAMVILENR
                     DANILNKSHQAADRYADLMQSGLALPLWNVAPNLGQPIIDSVKLDPAVSKIQVKTSDQ
                     ELFISYDAGSVAGASSADLIHIVRPIHYNQQNLGTLDLIYSLEPARQKASQETKQLIS
                     IISMQLIISLTVLIGFLHRRIIHPLSLLRTFANGIAQGNLRSEVPSLKKDELGDLAID
                     LEKMRQSLESNIQNLELHVNERTQELSQSNQQLSTTLEQLKLTQGHLIQSEKLAALGA
                     LVAGVAHELNTPIGNGLSVASALTHTCQNFAESLQKGMTKSALQAFVADVTEGNEIIS
                     RNLNKASELVSGFKQVAMDRTSAQRRSFKLLEFLNDTYLTLTPTFRRTAIKVVIECPE
                     DIELNSYPGPLGQVITNLLNNTLIHAFDADASGLVSIHGSMEDTQVCIRVSDNGKGIA
                     PEHLGKIFDPFFTTKLGKGGNGLGMHIVHNIVSGLLGGSIQVQSSLGAGCCFTLLLPQ
                     TAPATHAE"
     gene            3060..>3907
                     /locus_tag="PKZ52_13470"
     CDS             3060..>3907
                     /locus_tag="PKZ52_13470"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HNG60827.1"
                     /translation="MTQLPPDDDELFIFAEEDESTDLLVPPQERNNYWKILIVDDDKE
                     VHDATEFTLRGTLILNRPLEYVHAYSAKQAEALLREHTDIAVILLDVVMETPNAGLDL
                     IKTIRGPLANTQTRIILRTGEPNQAPELQIIRDYDINDYKLKSELNQKRLYTSLTSAI
                     RGYRQICTIEASKKGLDLILRSSSELLTQEGLRAFAQGVIIHIAALLNVEPEGIICVR
                     RRDSQPTHELHIIAAAGDYCPLLDRQLCDDNQTSIYSLLNQSIDKRCNIFEAAGMALY
                     LGCNAQG"
BASE COUNT         1125 a         1008 c          849 g          925 t
ORIGIN      
        1 ccggataaaa tgcgcggcac agagttaaaa tggcgttcaa acaagggcaa caactcaccg
       61 ttctgctcaa gctgcgcaaa gcctgcgcgc agacgctcaa gcaaatgctc ttcagacgca
      121 ggaaaaaaca gaaaagaagg cagcggataa aaaatcatta aatcatccaa cactattaaa
      181 tccttaagct caggtgcccg ctcggagaaa actcgatccg tctccagcac actcaagggc
      241 aaataatcga agcgtttttt ttgcagcatg ggcattaaat taataaaact ctgcccttca
      301 ttcaccacaa taccgttcga gcggtagata ttcacatcca gccagcgggt gccctgccca
      361 caacttaagc tcttaaattg ttgcaatgaa ttgatcttgg cgaatacatc ctggtcagag
      421 gcgcgcacaa ttaattgccg caacccgtgc aagccataat aaataggcag ctctagggac
      481 aaaacctgct catcacttac gcccaagccc tgccactcag aggcaaacaa tacattcacc
      541 ttatcgccct gctgcagggt gagcttcgag cggttagtac ttaacggcac tgaaaacaga
      601 tccaagctat aggcgccaaa ttccggcacg gtgttttcta gcatcagctg cagcaaatct
      661 ttttgatagg ctttaatgct gggggtatac ccctggtcgt aatggcgcac atgcatcacc
      721 tcatcagctc ccagcgccaa agcattggca accataagca cccacaaaaa accatagcga
      781 attagacaaa tttttggcat acagatattt aaaagctaat ttctcttaga gactatagct
      841 aacgactgaa gaacatatga cctaagctga caaaaaattg ccgtatgcat cactacacca
      901 agttatgcat atttatttac ccgccagcta accatgcgat gaacattcgt attcgctata
      961 taggaaccgc ttatataagt cttaaagtgg gaacattttg aaaccgtcgc aggcagaaag
     1021 ctctgcgatt taagctagag ggatggactt atacgctttc aaaatattca tgaattacca
     1081 aagaattaat cgcgttcgcg cagtcctcac gccttaaaca ggcaacaatg ggtggccatt
     1141 acagccatga gcgccacgta ataaatctta taatcgcaat cctgtaattt gcatctatag
     1201 ttaaacctgg taaccgccgc cgagtaattt atagctattt ataattaaca gtgatttttc
     1261 taaccctatt tgtaaggcag cgttcagcgg ttgacgccgc aaatcgttgc cgcaattgag
     1321 tcaaactatt taccgcaacc gggagctgta atgaggtact ggatttacaa cgggaaagag
     1381 ccaataaatg gctttcacta tggagacacc tacaggccta taggatcatt catcctatgc
     1441 ctgctatgag gctgttcaaa acacaactgg cttgccagca ggtacagccc gtgggcaaaa
     1501 agaatttttt ccagcgcctg atgcgtgaaa atcatctaca gcgggagtta attatttggg
     1561 taagcctggc ggtggtattg cccggtgccc tgatggccgc catggtgatt ttagaaaacc
     1621 gcgatgccaa tatcctcaat aaatcccacc aggccgccga ccgctacgcg gatttaatgc
     1681 agagtgggct tgcactgcca ctgtggaatg tggcgcccaa tctcggccaa ccgattatcg
     1741 acagcgtaaa attagacccc gccgttagca aaatccaagt taaaaccagc gatcaggaat
     1801 tgtttatctc ctatgatgca ggatcagttg ccggcgcctc cagtgcggat ttaattcaca
     1861 tcgtaaggcc catccattac aaccaacaga atttaggcac cctggattta atctacagcc
     1921 tggagcctgc gcgacaaaaa gccagccaag aaaccaaaca gctgatcagc attatcagca
     1981 tgcaattaat tatcagccta acggtattaa ttggcttttt acaccgccgc atcattcacc
     2041 ctctaagttt gctgcgcacc tttgccaacg gtatcgccca gggtaattta cgctctgaag
     2101 tacccagcct taaaaaagat gagctggggg atctggccat cgacttagag aaaatgcgcc
     2161 aaagcttaga atccaatatc caaaatttgg agctgcatgt taacgagcgc acccaggaat
     2221 taagccagtc caaccaacaa ctctccacca cactggagca attaaagctc acccagggcc
     2281 acctgattca atcggagaaa ctcgccgcct taggcgcctt agtcgcgggg gttgcccatg
     2341 aattaaatac tccgattggc aatggtttat cggtggccag cgccctgacc cacacttgcc
     2401 aaaattttgc cgaaagcctg caaaaaggca tgaccaaatc cgcgctgcag gcatttgtgg
     2461 ccgatgtaac ggagggcaat gaaattatca gccgcaattt aaataaggcc tcggaactgg
     2521 tgagcggctt taaacaggtg gccatggata gaaccagtgc acaaaggcgc agctttaaat
     2581 tattggaatt tttaaacgat acctatctca ccctcacacc caccttcagg cgcacagcaa
     2641 ttaaggtggt catcgaatgc cctgaggata ttgaattaaa ttcctacccc ggcccattgg
     2701 gccaggtaat taccaatctt ttaaataata ccttgattca cgccttcgat gcagacgcca
     2761 gcggtttagt ctccattcac gggagtatgg aagatacaca ggtgtgtatt cgcgtcagcg
     2821 ataacggcaa gggcatagcc cctgaacatt taggcaaaat ttttgatcct ttttttacta
     2881 ccaaactggg caagggcggc aatggcttgg gcatgcatat agtgcacaac attgtctccg
     2941 gtttattagg cggttctatt caggtgcaat ccagcctcgg cgcaggctgc tgttttaccc
     3001 tgttattacc gcaaaccgcc cccgccacac acgcagagta attacccaca ggttgatgta
     3061 tgacccagct gccccccgat gacgatgaat tatttatttt tgctgaggaa gatgaaagca
     3121 cagatttatt agtaccacca caggagcgca ataattactg gaaaattctc atagtagatg
     3181 atgataaaga ggtgcacgac gccaccgaat ttaccctgcg cggcacgctt attctcaacc
     3241 gtccactgga gtatgtacac gcctactcgg ccaaacaagc cgaagcgcta ctgcgcgagc
     3301 acacggatat tgcggttatt ttacttgatg tggtgatgga aacccccaac gccggtttgg
     3361 acctgattaa aaccattcgc ggcccattgg cgaacacgca aacgcgcatt attttgcgca
     3421 ctggcgagcc caatcaagcg cccgagctac aaattatccg cgactacgat attaacgatt
     3481 acaaattaaa atcggagctg aaccaaaaac gcctttacac ctcactaacc tctgctattc
     3541 gcggctaccg ccaaatctgc accattgagg cgagcaaaaa aggcttggat ttaattctgc
     3601 gctccagctc cgaattgctc acccaagagg gattacgagc ctttgctcag ggcgtgatta
     3661 ttcatattgc cgccctgctg aatgtggagc cggaaggtat tatttgtgtg cgccgtcgcg
     3721 atagccagcc cacccacgaa ctgcatatta ttgctgcggc tggggactat tgccccctgc
     3781 tcgatcgcca attatgcgac gacaatcaaa ccagtattta cagcctactc aaccaaagta
     3841 tcgataaacg ctgtaatatt tttgaagcag ccggcatggc gctgtattta ggctgcaatg
     3901 cccaggg
//
LOCUS       DAOQGS010000394         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12897_length_3905_cov_4.372208, whole genome shotgun sequence.
ACCESSION   DAOQGS010000394 DAOQGS010000000
VERSION     DAOQGS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12897_length_3905_cov_4.372208"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2671
                     /locus_tag="PKZ52_13475"
     CDS             <1..2671
                     /locus_tag="PKZ52_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG60828.1"
                     /translation="PDLRHKIQVRLADIEMARSQNRQLQAADGVHFAQAISLYDELLK
                     QPQTQKDAATDERLLYRLSKAYALDGKSAESEAILAQLVAAHPESNYAAEAEFRRGEQ
                     AFNLRQYGKAERSYQQVVKAGKSTPFYLNAVYMQGWSQFKQNKASEAIDSFTTLLDLL
                     LPAGVSVEQLDGGQKNLAEDTLRVLAYTFAYQEGAASITQAYKTRGERPYQALIYGQL
                     ADYYLEKKRYKDSADTYQNFIGQFPNNDLGPEYFVRMIEVYKVGGFPSEILPAKEAYV
                     NNYGVNSSFWASKNERQREPLKVHLKQFLDELSSYYHAQAQQQTAASKLPATKRKTPE
                     PQTVAANNVAASASAASLYLKAAAYYNQFITAFPQDAAAPNMTFLMAEAYADAGSMAD
                     AVKAYEKVAYEYKDASKGGEAGYSALIILDQLVKSTAGEAQKQWQAHKTQSAILFADN
                     YAKDNRAVAGLANATDDLFQRGDKTQALQLAQRVVNWQPAASSAQQKTAWLVIAHTQF
                     DLLQYKEAEEAYRKALALMPQSDPQYKQVSERIAASMFKAAETKVASQNQTAAVEQLL
                     QISTVAPGTDIAIKAQYDAANLLMDMKQWASAEQVLVDFNRRYPQHALAATVAPKLAL
                     IYQELQQWDKAADALAIMAKNDANPEARRSSLFLSAELARKSGKTQDAITRYGDYVRQ
                     YPRPFDVATEARFQLMELNQKMGDSKAAAGWQKALVDADAAAGSERTPRSRFLAASAN
                     SQYADNEMAAFNAVRLTAPLKDSIKVKRAAMDKALAAYKQVIDSGVPELTTKANHNIG
                     EIYAGLHRDLLKSERPAGLDSLATEQYEIMLEEQATPFKEKAVAMLIANSERASKGVY
                     DEWVKKSFTELAKLLPARFGKQEQILEVSNGLE"
     gene            2658..3350
                     /locus_tag="PKZ52_13480"
     CDS             2658..3350
                     /locus_tag="PKZ52_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG60829.1"
                     /translation="MASNKRANHFLSLLCALLLLAGCAETPSKEAKPEAITPDPSGKI
                     AQVIPNPYLQHAGDAPAAAKTEFTRIQGLIKNGRSADAITALTKMRQDYPKLSGPAVN
                     LALLYWKQKNYAQAQTAFEQAQGINKYNPDAYTQYAIMLREQGKFTEAEAQYLKALAV
                     WPHNVQAHRDLGVLYDLYLVQPEKALQHFTLCDQLSAEPNKEIKGWIIELNRRLNKKP
                     APAPEAKLEGAP"
     gene            3347..3643
                     /locus_tag="PKZ52_13485"
     CDS             3347..3643
                     /locus_tag="PKZ52_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60830.1"
                     /translation="MKKYLALLLLTSFAQAAEKSPPPAPSASGDDLSISIHGNQEQPT
                     VMHIVPWQEAGDNKIIQEVNKPPLNDLFPQIERSEHQREVRYLLNGDQSEKAER"
     gene            3716..>3905
                     /locus_tag="PKZ52_13490"
     CDS             3716..>3905
                     /locus_tag="PKZ52_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG60831.1"
                     /translation="MGFLDGVIKFFQDGGSHMYPIALAMIAGWAMVIERWIYLHKSKS
                     VNRDAFERMLPLLKKKDFN"
BASE COUNT         1098 a          962 c          964 g          881 t
ORIGIN      
        1 ccctgatctg cgccataaaa tccaagtgcg cctggcggat attgaaatgg cgcgcagcca
       61 aaaccgccaa ttgcaggcgg cagacggtgt gcattttgcc caggctattt ccctctacga
      121 tgaattatta aaacagccgc aaacgcaaaa agatgccgcc accgatgagc gtttgttgta
      181 tcgcttatcc aaagcctatg cgctggatgg caaatccgct gaatcagaag ccattttggc
      241 ccaattagtg gcggctcacc cggagtccaa ttacgcggcg gaagccgaat ttcgccgcgg
      301 cgagcaggcg tttaatttgc gccaatacgg caaggcggag cgctcttatc agcaggtggt
      361 aaaagccggt aaaagcacgc cgttttattt aaatgccgtg tacatgcagg gctggtccca
      421 gtttaagcaa aataaagcct ctgaggcgat tgattcattt accaccctgc tggatttatt
      481 attgccagcg ggcgtaagcg ttgagcaatt agacggcggc caaaaaaatc ttgctgagga
      541 tacactgcgg gtgctggctt atacttttgc ctatcaagag ggcgccgcgt ccattaccca
      601 agcctataaa acccgcggtg agcgccccta ccaagcatta atttacgggc agctggcgga
      661 ttattattta gagaaaaagc gctacaagga ttctgctgat acctatcaga attttattgg
      721 ccaatttccc aataatgacc ttggccccga atattttgtg cgcatgatcg aggtgtataa
      781 agtcggcggc ttccccagtg aaattctgcc cgccaaagaa gcctatgtga ataattacgg
      841 cgtgaatagc agcttttggg cgagcaaaaa tgagcgccag cgcgaaccgc ttaaagtgca
      901 tttaaaacaa tttttggatg aattatccag ctattaccac gctcaagccc agcagcaaac
      961 ggctgccagt aaattgcctg caactaaacg caaaacccct gagccgcaaa cagtggccgc
     1021 caataatgtc gccgcgagtg cctctgccgc cagtttatat ttaaaggccg ccgcttatta
     1081 caatcaattt attactgcct ttcctcaaga cgctgcagcg ccaaatatga cgtttttaat
     1141 ggcggaagcc tacgccgatg caggttccat ggctgatgcg gtaaaagcct atgaaaaagt
     1201 cgcctatgaa tataaagatg caagcaaagg tggcgaagcg ggttacagcg cgttaattat
     1261 tttggatcaa ctagtgaaaa gcactgcggg tgaggcgcaa aaacaatggc aagcgcacaa
     1321 aacccaaagt gcgattttat ttgccgataa ttacgccaaa gataaccgcg ctgttgcagg
     1381 ccttgccaat gccactgatg atttattcca gcgcggcgat aaaacccagg cgctgcaatt
     1441 agcccagcgg gtagtgaatt ggcagccagc ggccagcagc gcgcaacaaa aaacggcctg
     1501 gctggttatt gcccataccc aatttgattt attgcaatac aaagaggcgg aagaagccta
     1561 ccgcaaagcc ctcgcattaa tgccgcaatc ggacccgcaa tataaacagg tgagtgagcg
     1621 cattgccgcc agtatgttta aagcggcgga gaccaaagtg gccagccaaa atcaaaccgc
     1681 ggcggtggag caattattac agattagcac tgtggcaccg ggcactgata tcgccattaa
     1741 agcccagtat gacgcggcga atttattaat ggacatgaag cagtgggcga gtgcagagca
     1801 agttttagtg gattttaatc gccgctatcc gcaacacgcg cttgccgcca cggtggcgcc
     1861 caaactggcg ctaatttatc aagaattaca gcagtgggat aaggctgccg atgccctagc
     1921 cataatggcc aaaaatgacg ccaaccccga ggcgcgccgc agttcattat ttttatcggc
     1981 agaactggcc cgtaaatccg gtaaaactca ggatgccatc acccgctacg gcgattatgt
     2041 gcgccaatat ccgcgcccgt ttgatgtggc taccgaagcg cgctttcaat taatggaatt
     2101 aaatcaaaaa atgggcgaca gtaaagccgc cgctggttgg caaaaagctc tggtggatgc
     2161 cgatgctgcc gcaggcagcg agcgcacccc ccgctcgcgc tttttggcgg cctccgccaa
     2221 cagccaatat gcggataatg aaatggccgc ttttaatgcg gtgcgcttaa ccgcgccctt
     2281 aaaagacagt attaaagtta aacgcgccgc tatggataag gctttagccg cctataaaca
     2341 ggtaattgat tcaggcgtgc cggaattaac cactaaggct aaccataata ttggtgaaat
     2401 ttacgcaggc ttgcaccggg acttattaaa gtctgaacgc ccggcaggtt tggacagttt
     2461 ggctacagag caatacgaaa ttatgctgga agagcaggcc acacccttta aagaaaaagc
     2521 ggtggccatg ttaatcgcca acagcgagcg cgcttcaaaa ggtgtgtacg atgaatgggt
     2581 gaaaaaaagt tttacggaat tagccaaatt attacccgcc cgttttggca agcaagagca
     2641 aatattggag gtaagcaatg gcctcgaata agcgtgctaa ccatttttta agcctgctat
     2701 gcgccctttt gttgttagca ggttgcgccg aaacccccag taaagaagct aagccggaag
     2761 caataactcc tgatccgagc ggcaaaatag cccaggtaat acccaaccct tatctgcagc
     2821 atgcaggcga tgcccccgcc gcggccaaaa cagaatttac ccgcattcag ggcttaatta
     2881 aaaatggccg cagtgctgat gccattacag ctctcaccaa aatgcgccag gattatccta
     2941 aattatcagg ccctgcggtg aatttggcgc tgctgtattg gaaacaaaaa aattacgccc
     3001 aggcgcaaac cgcttttgaa caggcccaag gcattaataa atacaaccca gatgcctata
     3061 cccagtacgc catcatgctg cgggagcagg gcaagtttac cgaggccgaa gctcaatact
     3121 taaaagccct tgcggtgtgg ccccacaatg ttcaggccca ccgagattta ggtgtgttat
     3181 acgatcttta tctggtgcaa ccggaaaaag ccctacagca ctttaccctg tgtgaccagt
     3241 taagtgctga acctaataag gaaataaaag gctggataat agaattaaat cgtcgcttaa
     3301 acaaaaaacc agcccccgcg ccggaagcta aacttgaggg agcgccatga aaaaatacct
     3361 cgccctattg ctgttaacct cttttgccca ggcggcggaa aaatctccgc cgccggcacc
     3421 ttctgcgtca ggggatgatt tgagtatcag tattcacggc aaccaagagc agccgactgt
     3481 aatgcacata gtgccttggc aggaggccgg ggataataaa attattcagg aggtgaacaa
     3541 accaccgctg aatgatttat ttccccaaat tgaacgcagc gaacatcagc gtgaagttcg
     3601 ctacttgcta aatggtgatc agtctgaaaa ggcggagcgc tagaattttt gaatctgctg
     3661 cgctaattgc agcatgtact aacaattaat gtaatgttcg gagtttggag tttttatggg
     3721 ttttttagat ggtgtaatta agttctttca agacggtggc agccatatgt accccatcgc
     3781 cttggccatg attgcaggct gggccatggt gatcgagcgc tggatttatc tgcataaatc
     3841 caagtccgtc aaccgcgatg cgtttgagcg catgttgccg ctgttaaaaa agaaagattt
     3901 taacg
//
LOCUS       DAOQGS010000395         3896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12943_length_3896_cov_5.568602, whole genome shotgun sequence.
ACCESSION   DAOQGS010000395 DAOQGS010000000
VERSION     DAOQGS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12943_length_3896_cov_5.568602"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..155
                     /locus_tag="PKZ52_13495"
     CDS             3..155
                     /locus_tag="PKZ52_13495"
                     /inference="COORDINATES: protein motif:HMM:NF017018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HNG60832.1"
                     /translation="MLTPERTLALASAQKVMEAIAEQGFYLQMPPQADEEMRRIHQHN
                     SKLYGL"
     gene            164..376
                     /locus_tag="PKZ52_13500"
     CDS             164..376
                     /locus_tag="PKZ52_13500"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNG60833.1"
                     /translation="MISEEAWIDVQMRLAFQEDALAQMSQQMAWQAEELKKAQGHILL
                     LNQKLNDLLSALDNQNPIIDQRPPHY"
     gene            385..726
                     /locus_tag="PKZ52_13505"
     CDS             385..726
                     /locus_tag="PKZ52_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60834.1"
                     /translation="MYSHDEYVMGWMYYGAGVAVFLLAWWFLTRPIAFNELRQLLRFI
                     PAVALIVPWHADPDSIYLAPAWVVAITEGIFEGPAAFWRAGTPLLFAMVLMLIMVTIV
                     KLIGFFIRSRD"
     gene            898..1614
                     /locus_tag="PKZ52_13510"
     CDS             898..1614
                     /locus_tag="PKZ52_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60835.1"
                     /translation="MNFLLPPNSTLLWLSLALCLPALCIAVVYAPWRLLLASQARQHL
                     FMASTLGLSLVWLFGVTVKNTIIFHPILMTVTTMVLGWSLAQLAGLSALLLLLLFQTA
                     LRSALVGWDMAYSQLHLSTVALDFCLNVLVPASWTWCVIAAVNRWQFKNPFTYFLGVG
                     FFGAMLGCLWVDLAALAMCYWAGSAAHWVIIKDHFWLFLLMTFPEGFINGTIATAFTV
                     FMPDLVKTYKESWFVQDDKN"
     gene            1728..2963
                     /locus_tag="PKZ52_13515"
     CDS             1728..2963
                     /locus_tag="PKZ52_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60836.1"
                     /translation="MNRDCFCVTLNQQQLRAQLLSDQLSQDLLLTHPHLFAQTSVFLS
                     PDHFQQIQRVIAAIGRVVKRPEFTQQVLHLPADNSCFTAQPLGVFMGYDFHLGAAGPK
                     LIEINTNAGGAFLNALLVDAQSACCRSTHVEALTKESLHQQFYAMFQNEWQLMRGDKP
                     LKTMAIIDVQPEQQYLYPEFKMAQHLFARHGITALIADPSELCIQGNALCVLQTGQLH
                     EIDLVYNRLTDFALKEHTHLALRQAFEADLAVITPNPNLHARYADKRNLCVLSDECRL
                     RALGVSEGDIAVLKEAIPATRLVKPEDADELWAARKQLFFKPWAGFGGRAAYRGDKLS
                     LRIWQEIVHGHYIAQQVIPPSERGILVDGNETTLKMDIRAYVYDGAIQLLAARLYQGQ
                     TTNFRTPGGGFAPVYIMSR"
BASE COUNT          896 a          900 c          968 g         1132 t
ORIGIN      
        1 tggtgctaac ccctgagcgt acactcgccc tcgccagtgc ccaaaaggtt atggaggcca
       61 ttgccgagca gggtttttat ctgcagatgc cgccccaggc cgatgaagaa atgcgccgta
      121 ttcaccaaca caacagcaaa ctctacgggt tgtaaccagc gatatgatca gcgaagaagc
      181 ctggattgat gtgcaaatgc ggttagcgtt tcaagaagat gccctagcgc agatgagcca
      241 gcaaatggcc tggcaggcgg aagaattaaa aaaagcccag ggccatattt tattgctcaa
      301 ccaaaaactc aacgatttat taagcgcgct ggataaccaa aaccccatta tcgatcagcg
      361 cccaccgcat tattaaggct tagcatgtac agtcacgacg aatatgtaat gggatggatg
      421 tattacggcg ccggtgtggc ggtattttta ttggcctggt ggtttttaac ccgccccatt
      481 gcatttaacg aactgcgcca attattgcgc tttattcccg ccgtcgccct tattgtgccc
      541 tggcacgccg acccagactc catttacctt gcgcccgctt gggtggttgc cattaccgaa
      601 ggcatatttg aaggcccagc cgcattttgg cgcgccggta cgcctttatt atttgctatg
      661 gtgttaatgc taattatggt caccattgtg aaattaattg gcttttttat tcgcagccga
      721 gattaacact tatttaaata ggtacgcggc gatggggtgt atttaaagcc tgttgcaaca
      781 gggcgcgctt ttggcgctgt ggttttttgc tggatgcgtt gttacgcaga gtatttctcc
      841 cttaattagc atttatagca gtccttcagc ctgttggtcc gacgagagca aatgcttatg
      901 aattttctgt tgccgcccaa tagcacatta ttatggttaa gcttagcgct ttgcctgccc
      961 gcactgtgta ttgcggtggt atacgcacct tggcgattat tgctcgcctc ccaggctcgg
     1021 caacacttat ttatggccag cacattgggt ttaagcctgg tgtggttgtt tggtgtcacg
     1081 gtaaaaaaca ctattatttt tcaccctatt ttaatgacgg tcaccaccat ggtgttgggt
     1141 tggagcctag cgcaactggc tgggcttagc gcattactcc tcttgttatt atttcaaacc
     1201 gctttgcgct ccgccctggt gggttgggat atggcctatt cacaactgca tttaagtact
     1261 gtggcgctgg atttttgcct caatgtatta gtgcccgcca gttggacctg gtgtgtaatt
     1321 gcggcggtta atcggtggca gtttaaaaac ccttttacct attttttggg ggtaggtttt
     1381 tttggcgcca tgctcggctg tttatgggtt gatttggccg ccctggccat gtgttactgg
     1441 gcgggcagtg cggcccattg ggtcatcatt aaagatcatt tttggttgtt tttattgatg
     1501 acctttccag aagggtttat caacggcacc attgccaccg catttacggt atttatgccg
     1561 gatttggtaa aaacctataa agaatcctgg tttgtgcagg atgataaaaa ttgaatgctg
     1621 gtaaaccaaa ttgttaattg agcaagcatc gctacccaca acatctattg ccgagacaac
     1681 ttgtgccttc ctcacattcc aagcaaccgc cgagctctgc agaagccttg aatcgagact
     1741 gtttctgcgt cactttaaat caacagcagt tgcgcgcgca gctactgtct gatcagctca
     1801 gccaggattt attgctgact cacccgcact tatttgcgca aaccagcgtt tttttatcgc
     1861 ctgatcattt tcagcaaata cagcgcgtga ttgcggctat tggccgggtg gttaagcggc
     1921 ctgaatttac ccaacaggtg ttacacctgc ctgcggataa tagctgcttt accgcccagc
     1981 cgttgggtgt ttttatgggc tacgattttc acctgggcgc cgctgggccc aaattaattg
     2041 aaattaatac caatgcaggc ggagcgtttt taaatgcgct cttggttgat gcccagagcg
     2101 catgctgtcg ctccacgcat gttgaggcgc tcaccaaaga gagtttgcat cagcagtttt
     2161 atgccatgtt tcaaaatgaa tggcagttga tgcggggcga taaaccgctt aaaactatgg
     2221 ccattattga tgtgcaaccg gagcaacagt atttatatcc cgaatttaaa atggcgcagc
     2281 atttatttgc ccggcacggc ataacagccc taattgccga ccccagcgag ttatgtattc
     2341 agggtaatgc cctctgtgtc ttgcagaccg ggcagctcca tgagattgac ctggtctaca
     2401 atcgcctgac ggattttgcg ctcaaggagc atactcattt ggccttgcgg caggcgtttg
     2461 aagcggatct ggcggtaatt acccccaatc ccaacctcca tgcccgctat gcggataaac
     2521 gcaatttatg tgtactcagt gatgagtgca ggctaagggc cttgggggtg agtgagggcg
     2581 atattgcggt gttaaaagaa gccataccgg ctacgcggct ggtgaagcct gaagatgctg
     2641 atgaattatg ggcagcgcgc aagcagctat tttttaaacc ctgggcgggt ttcggcggcc
     2701 gagcggccta tcgcggcgat aaactaagcc tgcgtatttg gcaggaaatt gtgcatggcc
     2761 actacattgc gcagcaagta ataccgccca gtgaacgcgg tattttggtg gatggcaatg
     2821 aaaccacact taaaatggat atacgcgcgt acgtgtatga tggtgcgatt cagttgctcg
     2881 ccgcgcgtct ttatcagggc caaaccacta attttagaac cccaggtggt ggttttgcac
     2941 ctgtatacat aatgtcgcgt tgatattggt ggtatctgat ttaaggctgg agttatttta
     3001 tttttcgcct aactatttcg gcatctttta tcagcatctt tttaagaggc taaagggaat
     3061 tccagcactg aatgggaatc atcaagtgcg tgttttagtg ctgcgacccc ctaatactgc
     3121 attgccgtga gtcgattttg ccccatggct aaatcctcta gggaacctct gaaaaattac
     3181 cttactcgct ctggcgtcgt tgaatttgcc aaaaaaatgc tcatttactg cgtgtaaact
     3241 ccgctttttt tggcaaattc gcctagccag agcttcgccg ccgacatttt tcagaggttc
     3301 cctagaattt tccagccgcg cgaaagaggt aattaaggcg tgctgaatgg ttacattttc
     3361 gcaagcatga agcctgggag ggcgctacag cggcgcattt caatttgttc attcctaagc
     3421 ggcttattaa ccaagtggtt aaataatatc tcggcagtct gtaacttagg cagattaccc
     3481 tatcaatttg tgtgcttatc agcctatcaa tcggctttta ttcaattggc agttgtttta
     3541 gcgttgatgt aaagagcgat ttaatcttaa gtgttcagcg caggtttttt aatagctcgc
     3601 tgatgctaaa acactcctgc agatttacaa atgatcaaag aataagcgcg cttttagtcc
     3661 cgtaattaca tcctccagca tataattaat ctgtgcagcg aatcctgctg tgctctttac
     3721 cgtgctgcgt gatactactg ccggaggtgg cctatgactg ttgctgatgt atgtgtttat
     3781 gtggtgatga cgttgagtgt tttgactgtg tgtgcatctg cacttgatcc tgcatagccc
     3841 cagggcccag caggctcata tctatatgca gtggcggtga tggtgtgagc gggggc
//
LOCUS       DAOQGS010000396         3888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_12980_length_3888_cov_5.456561, whole genome shotgun sequence.
ACCESSION   DAOQGS010000396 DAOQGS010000000
VERSION     DAOQGS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12980_length_3888_cov_5.456561"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKZ52_13520"
     CDS             <1..985
                     /locus_tag="PKZ52_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60837.1"
                     /translation="LASNGLLAIGAFDLFDDDRTLSLTAGQLQVGLSAASGQNLWNLA
                     VDEADIALVGSADLTLTDSQGLSLRDLNNDGKSLALSDGNLALRLASGDLSINADLLA
                     QDASNDSRTAGTIDVAVAAGSISSRGALSITARAASPQPPGSFSLRFVLEDNSAADRS
                     IRLGAGTHLTAVGGDILMDTRPQNTAKGAHRTYVQEQALGALPAAEVSAYTNNNDPTT
                     GRVTLNGAPLAASKQQSIGANRWLAIVADGDTDYAGALEQTEDLSQATKAIEQVNKGG
                     LTVDEQFGKVFGDCDEFDPKTRHRCKVNSALKAFLSHWLVGGELPSKREPD"
     gene            985..1596
                     /locus_tag="PKZ52_13525"
     CDS             985..1596
                     /locus_tag="PKZ52_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNG60838.1"
                     /translation="MPQYGRIFFKLVLAVLLHTACVSAQANNDKREAELQVRAQFIYN
                     FANFVEWPKDAFANPEAAIKVCLFGEVDFAPYLFAFADTLIGSRPLKIQRANEIAEIR
                     AGCHILYVGQDERVKLPTFWQQIHYIYVLSIGDRQGFADKGGIINILRAQDKMQFEVN
                     IENALANGLFLDSDLLALARVIKRNTAMGDNANAAPVNNGKGN"
     gene            1652..3607
                     /locus_tag="PKZ52_13530"
     CDS             1652..3607
                     /locus_tag="PKZ52_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60839.1"
                     /translation="MRYYRDLPLQRKISTIVAVGMTAVTLLTLLYFFAFDIGNLKRDL
                     GEEIRVLARITAARSAAAVAFGDGINAAENLKTLELRSSIQHACIFDQNQQLFASYRR
                     PNSPFEPCAASFNGAFSPSLAQLKDRIEVAEPIARKNQQLGIVLITADLSSIAERKHN
                     WLLTCALVMVFAPFITYLMTLRLKSSVVKPILDLADVMDLVRSSNNLSLRAQVLGRDE
                     VGSLGESFNEMLRIIETNNRDLEHLYRVSVEKSAEAEAAAASLQIRNQQIKDLFGGAA
                     HDLRQPLQAMAIFVQTLGRKIHDPEQLIIVDKLRQATQNLSGLFKEVLDVARYEFDQQ
                     VAATQSVAIKPLLNKLYLEFEALAQEKGLRLHFFTPDNKILAHAILLERIIRNLLSNA
                     IRYTDKGGVLLGCRKRGNSLAIEVWDTGRGIPLNQQEQIFHKYVQVSEEDRDLRGGFG
                     MGLAIVKQFVDSLGYKLSVRSVPNRGTVFRLLVPLVTSNRRPAKLNPRPAPRTPALIP
                     ASVVLPPAQLKMADMRERKELRILLLDDHDDVRWGIKQHLEEWGFAVNDFASEQAATA
                     FYRTGGKKPDLIISDYELGPAHTGTQAIEALRRTLGEATPAFIVTGADDPDIWQAITA
                     SGYFAISKPFKPARLRALINHLLLVQR"
BASE COUNT          921 a         1101 c         1030 g          836 t
ORIGIN      
        1 ccttgccagc aacggtctgt tggcgatcgg ggcgtttgat ctattcgatg acgaccgcac
       61 actcagcctc accgccgggc aattacaggt aggcctcagc gctgcaagcg ggcaaaatct
      121 gtggaatcta gcggtggatg aggcggatat cgccctagta ggcagcgcgg atttaaccct
      181 caccgatagc caaggcctca gcctgcggga tttaaataac gacggcaaaa gccttgcctt
      241 aagcgatggc aacctggccc tgcggctcgc ctccggcgac ctcagtatta atgccgacct
      301 attggctcag gatgccagca acgatagccg cactgcgggc actattgatg tggcggtggc
      361 cgcaggcagt atcagcagcc ggggcgctct tagcatcacc gcccgtgcag ccagccccca
      421 gccccccggc agtttcagcc tgcgctttgt gttggaggat aactccgcag cggatcgctc
      481 cattcggtta ggggcgggga cgcaccttac cgccgtcggc ggcgatatct tgatggatac
      541 ccgcccgcaa aacaccgcca agggggccca ccgcacctat gtgcaagagc aggccctggg
      601 cgcgctgccc gctgccgagg ttagcgccta cactaataac aacgacccca ccacaggccg
      661 cgtcaccctc aatggcgcgc ccctagcggc cagtaaacag cagagcatag gcgccaatcg
      721 ctggctcgcc atagtggccg atggggatac ggattatgcc ggtgccctgg agcaaaccga
      781 ggatttaagc caggccacta aagccataga gcaagtgaat aaaggtggcc tgacggtgga
      841 tgaacaattt ggcaaggtgt ttggggattg cgacgagttc gacccgaaaa cccgccaccg
      901 ctgcaaggtg aattccgccc taaaagcatt tttaagccac tggttagtcg gtggcgaatt
      961 accctccaaa cgggagccag attaatgccg cagtatgggc gtattttttt caagctagtg
     1021 ttagctgtgt tgttgcacac ggcctgcgtc agcgcgcagg caaataatga taaacgcgaa
     1081 gctgaactgc aggtgcgggc gcaatttatt tataacttcg ccaattttgt cgagtggccc
     1141 aaagatgcct ttgccaaccc cgaggcggct attaaagtct gtttattcgg cgaggtggat
     1201 tttgcgccct atttatttgc ctttgccgac acgctcatcg gcagcaggcc gcttaaaata
     1261 cagcgggcca atgaaatcgc ggaaattcgc gcgggctgcc atattttata tgtggggcag
     1321 gatgagcggg taaaactgcc caccttctgg caacaaattc actatattta tgtattaagc
     1381 atcggcgatc gccagggttt tgccgataaa ggcggcatta ttaatattct gcgcgcgcag
     1441 gataaaatgc agtttgaggt caatatagaa aacgccctgg ccaacggcct atttttagat
     1501 tcggatttat tggccctggc gcgggttatc aaacgcaata ccgccatggg cgataatgcc
     1561 aacgccgccc cagtgaataa cggcaagggg aattagcctt gcagcggccg gtgttgtcaa
     1621 gccattgtgc gtgtgggggg cgttaaacca catgcgttat taccgcgatt tacccctgca
     1681 gcgcaaaatc tccaccattg tggcggtggg catgacggcg gttaccctgc taaccctgct
     1741 gtattttttt gcttttgata taggcaattt aaagcgcgat ttaggcgagg aaatccgcgt
     1801 cctcgcgcgc attaccgccg cccgcagcgc agcggcagtg gcctttggcg acggtattaa
     1861 tgcggcggaa aatttaaaaa ccttagagct gcgcagctcc attcagcacg cctgtatttt
     1921 tgatcaaaac cagcaattat ttgccagcta ccgccgcccc aatagcccgt ttgaaccctg
     1981 cgcagcaagt tttaacggcg catttagccc ttcgcttgcg caattaaaag accgcataga
     2041 agtagctgaa cccatcgccc gcaaaaacca acagctgggc attgtgctga taactgccga
     2101 tttatccagc atcgccgagc gcaaacacaa ttggttattg acctgcgccc tggtgatggt
     2161 gtttgcgccg tttattacct acctgatgac cctgcgctta aaaagcagcg tggtaaaacc
     2221 tattttggat ttggccgatg tgatggacct ggtgcgcagc agcaataatt taagcctgcg
     2281 cgcgcaggtg ctcggccgcg atgaagtggg cagcctaggg gaatccttta acgaaatgct
     2341 gcgcattatt gaaaccaata accgggatct agaacacctc taccgtgtgt cggtggaaaa
     2401 aagcgccgag gcggaagccg cagcggcatc gctgcaaatt cgcaatcaac aaattaaaga
     2461 tttattcggc ggcgccgccc acgatttgcg ccaaccctta caagccatgg cgatttttgt
     2521 gcaaaccctc ggccgcaaaa ttcacgaccc cgaacaatta attattgtgg ataaattgcg
     2581 ccaggctacg caaaacctaa gcggtttatt taaagaagtg ctggatgtag cccgctacga
     2641 atttgaccaa caggtggccg ccacccaatc ggtagccatt aaaccgttac tgaataaact
     2701 ttatttagag tttgaagccc tggcccagga aaaaggcctg cgcctgcatt tttttacgcc
     2761 ggataataaa atcctcgccc acgccatttt gcttgaacgc atcatccgca atttattgag
     2821 caacgccatt cgctacacgg ataaaggcgg cgtattactg ggctgccgca aacggggcaa
     2881 tagtttagct attgaggtgt gggataccgg gcgcggcatt ccgcttaatc agcaggagca
     2941 aatttttcat aaatatgtgc aggtgagcga agaggaccgc gatctgcgcg gcggctttgg
     3001 gatgggactg gccattgtga aacagtttgt ggatagcctc ggctataagt tatccgtgcg
     3061 ctcggtgccc aaccgcggca cggtatttcg cctcttagtg cctttggtca ccagcaatcg
     3121 ccgcccagcc aaactcaacc cccgcccggc gccccgcacc cccgcactca ttcccgcctc
     3181 tgtggtatta ccgcccgccc agttaaaaat ggcggacatg cgcgagcgca aagagctgcg
     3241 cattttgctg ctggacgatc acgacgatgt gcgctggggc attaaacagc acttagagga
     3301 gtggggtttt gccgtcaacg acttcgccag cgaacaggcg gccacagctt tttaccgcac
     3361 gggcggtaaa aagccggatt taattatctc cgactacgaa ctcggccccg cccacaccgg
     3421 cacccaagcc attgaagcgc tgcgccgcac actgggcgaa gccaccccgg catttatcgt
     3481 caccggcgcc gacgacccgg acatttggca agccatcact gccagcggct acttcgccat
     3541 cagcaaaccc ttcaaacccg cccgcctgcg ggctttaatc aatcatcttt tattggtgca
     3601 gcgctaagta cccagcagca cctgccgccc agaaaaaatt gttaagagtt ctgcgggcgc
     3661 ccctcatagg ccccgcttgg gcctgcgggg aaaagtcggc gactattttt cagttcaaac
     3721 tttgccccaa attcccctcg tctggagggg tgccccgaag gggcggggtg gtgggcgatt
     3781 cacaggagct tatctaccaa acccagcacc aacgcatatg cctcactatg aggcacaaaa
     3841 gccaagagct gctttcccat cgacacccag aaaacccaat taagactc
//
LOCUS       DAOQGS010000397         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13020_length_3882_cov_4.790698, whole genome shotgun sequence.
ACCESSION   DAOQGS010000397 DAOQGS010000000
VERSION     DAOQGS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13020_length_3882_cov_4.790698"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PKZ52_13535"
     CDS             <1..1017
                     /locus_tag="PKZ52_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60840.1"
                     /translation="AIFPSGAGVLHNSTKSHLIEALLEGSNLNVVCKSSAKAALDFIN
                     TRMPDLILMDIQMPEMDGYAATAHIRALVADANPIILAMTANNSPEDTQKSLAAGMQG
                     HLCKPIDADELIKQLGFWLHANTQGPVPTTPVSAPLKAITTDELPGIDLAAALVRLRG
                     NRTLLEEVLISFADKAALIIPELQQAIKTLDLDGATRILHRLKGTSANLSAVRVSPLA
                     ARLETQCKQGQLPNDDELIELESAIGELITTARGLYSAQSAQPISPTDVDYTAIKQCL
                     AQIGTHLNSDLGRAEDAVTALLQLSQNTPWAAAANQLRTLFYQFNLRAVHDLILNGFE
                     QEPL"
     gene            1014..2156
                     /locus_tag="PKZ52_13540"
     CDS             1014..2156
                     /locus_tag="PKZ52_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60841.1"
                     /translation="MSSQTKILIVDDSPEDIRLLMNELKGDFQITAATSADLALQMIS
                     ETLPDIVLLDVNMPGTDGYTACSIIKDSPETASIDVIFLSSNDSTDEVLNGFSAGASD
                     YLVKPYAPLILLSKINACIARRQDLHKLTEAAKYAGAVAMSAMSDTGDLGTIVNFLRS
                     SFAIKTADDLCREICSALSTFGLNACAHIYLDDGEVFGATEGAPSELEMNLIKRILNS
                     PEPIITVGNRLLLIKPGVVLLIKNMPEAAEKAARIKDHLMILLEGIVAKVISLNSQAK
                     LNQLLSSNINRVIKDAEHALIAIRENQEAHKKQSIDIMEKMVTEVEASFFNMGLTDQQ
                     EEKLLEIMTKAVSESLNHFEAGLEIDNKISGIIDSLSQVSSDAQGI"
     gene            complement(2212..3561)
                     /locus_tag="PKZ52_13545"
     CDS             complement(2212..3561)
                     /locus_tag="PKZ52_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HNG60842.1"
                     /translation="MNITCFKAYDIRGRVPDELNDDVAYRVGRAFAEYLNAKRLVVGH
                     DIRLTSPALTNALVRGMTDAGADVIHIGQCGTEEVYFATFALEVDGGICVTASHNPMD
                     YNGMKLVREGSKPISGDTGLLDIKALAESGQFKASARKGLVEQRDMRGAYVEHLLTYV
                     DTKALKPIKVVVNAGNGGAGAVVDALQPHLPFEFIKIHHDPDGNFPHGIPNPLLPENQ
                     GVTAEAVLQHGAAVGIAWDGDFDRCFFFDEKGNFVEGYYVVGLLAEAFLAKHPGAKIV
                     HDPRLTWNTVDVVNQAGGVPVQSKTGHAFIKERMRLEDAVYGGEMSAHHYFKTFAYCD
                     NGTIPWLLVLELMQQKNLPLSALVEERIARFPCSGEINRTVRNTREMLATVEAQFAAE
                     AVAIEHVDGLSMAFADWRFNMRSSSTEPLARLNVESRGDEALMRAKTQQLLALMDQF"
     gene            complement(3561..>3882)
                     /locus_tag="PKZ52_13550"
     CDS             complement(3561..>3882)
                     /locus_tag="PKZ52_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNG60843.1"
                     /translation="IGGEQIKNGIFQINTMVEKPKKEDAPSNLAIIGRYILTPDIFEI
                     IEQTKPGKGGEIQLTDALLTQAQKGCVIGYKFKGKRFDCGSVDGFVDATSFVYNNIYN
                     VKKG"
BASE COUNT          995 a         1007 c          948 g          932 t
ORIGIN      
        1 gccatttttc catctggcgc gggggtttta cacaattcta cgaagagcca tttaattgag
       61 gcgctgctgg agggcagcaa tttaaatgtg gtctgcaaat cctcagctaa agccgcctta
      121 gattttataa acacccgcat gccggatctt atactcatgg atatccaaat gcccgagatg
      181 gacggctacg cggcgaccgc gcacattcgc gctcttgttg ctgatgccaa cccaattatt
      241 ctggcgatga ccgctaataa tagcccggaa gacacacaaa aaagcttggc ggcaggtatg
      301 caagggcatt tgtgtaaacc cattgatgcc gacgaattaa taaaacaatt agggttttgg
      361 ttgcacgcca acactcaggg cccggtaccc actacacctg tcagcgcacc acttaaagcg
      421 attacaactg atgaattacc cggcattgat ttagcggccg ccttagtgcg tttgcgcggc
      481 aaccggacct tattagagga ggtattaatc tccttcgccg acaaagcggc acttattatt
      541 cccgaattac agcaggccat taaaaccctg gatttggacg gcgccacccg tatcttgcat
      601 cgcttgaaag gcacctcggc caatttaagc gccgtgcgcg tgtcgccact ggccgcacgg
      661 cttgagacgc aatgcaaaca aggccagctg ccaaacgatg atgagttaat tgagttagaa
      721 agcgccattg gggaattaat cactacagcg cggggcttat attcagcaca atcagcgcaa
      781 cccatatcgc ccactgacgt ggattacaca gctattaaac agtgcctggc acagattgga
      841 acacacctaa acagtgattt aggccgcgca gaggatgcgg tgacagcatt gctgcaattg
      901 agccaaaaca ccccatgggc cgctgcagcc aaccaactgc gcacgctgtt ttaccagttt
      961 aatctgcgcg ccgtacacga tctaatatta aatggctttg agcaagaacc cttatgagta
     1021 gtcaaaccaa aatattaatt gtcgatgatt cccccgagga tattcgatta ttgatgaatg
     1081 aactgaaagg cgattttcag atcaccgccg ccacatctgc agaccttgcg ctgcaaatga
     1141 tttcagaaac cctgcccgat atagtgctgt tggatgtaaa catgcccggc accgatggtt
     1201 ataccgcttg ttcaattatt aaagattcgc cggagacagc cagcattgat gtgatatttt
     1261 tatccagtaa tgactccacg gatgaggtgc tgaatggttt tagcgctggc gccagcgact
     1321 atttggtaaa accctatgcg ccgttaattt tattgtcaaa aattaacgcc tgtatcgccc
     1381 gcaggcaaga tttacacaaa ttgactgagg cggccaagta tgcaggtgcc gtggccatga
     1441 gcgccatgag cgataccggc gatttaggta ccattgttaa tttcctgcgt tcgagttttg
     1501 ccattaaaac agccgatgac ctctgccggg aaatctgctc ggccctatcc acatttggtt
     1561 taaacgcctg cgcgcatatt tatttggatg atggcgaagt gtttggcgca acggagggcg
     1621 cccccagcga attagaaatg aatttaatta aacgcatcct aaacagccca gaaccgatta
     1681 ttaccgttgg caaccggctg ctgctgatca aaccaggcgt tgtgttatta attaaaaata
     1741 tgcccgaggc ggcggaaaaa gccgctcgta ttaaggatca cctcatgatt ctgctggagg
     1801 gcatcgtcgc caaagtgatc agcctcaaca gccaggccaa actcaatcaa ttgctcagca
     1861 gcaatattaa ccgggtgatc aaagatgccg aacacgccct cattgctatc cgtgaaaacc
     1921 aagaggccca caaaaaacaa agcatcgaca tcatggaaaa aatggtcaca gaagtggagg
     1981 cctccttttt taatatgggc ctgacggatc agcaggagga aaagttgctg gaaattatga
     2041 ccaaggcggt gtctgaatcc ttaaaccatt ttgaggcggg cctggagatt gataataaaa
     2101 ttagcggcat tattgattcg ctgtcgcagg tctccagcga cgcgcaggga atttagcgca
     2161 ggatttctac aggcgaaaaa aatggccctt agggccattt ttattaggtg attaaaactg
     2221 atccatcagc gccaataatt gttgggtttt ggcgcgcatc aaggcttcat ccccacggga
     2281 ttccacattt aaacgggcca agggttccgt gctggagctg cgcatattaa agcgccaatc
     2341 ggcaaatgcc atggaaagtc catccacatg ctcaatagcc actgcttcag cagcaaactg
     2401 cgcttccact gtagccagca tttcccgcgt gttgcgcacg gtgcggttaa tttcgccaga
     2461 acaggggaat cgcgcaatgc gctcttccac cagcgccgag aggggcagat ttttttgctg
     2521 cattaattct aaaaccaaca accaggggat ggtgccgtta tcgcaatagg caaaggtttt
     2581 aaaataatgg tgggcgctca tttcaccgcc gtacaccgca tcttccaaac gcatgcgctc
     2641 tttgataaac gcatggccgg ttttactttg cacgggcacg ccacccgcct ggtttaccac
     2701 atccacggta ttccaggtta aacgcgggtc gtggactatt ttggcgccgg ggtgtttggc
     2761 taaaaacgcc tccgccaata gacccaccac ataatagcct tcaacaaaat tgcctttttc
     2821 atcaaagaaa aagcagcggt caaaatcccc atcccaggca atacccaccg ccgcgccgtg
     2881 ttgcaacacc gcttcggccg ttacaccctg attttccggc agtagcggat tgggaatacc
     2941 atgggggaaa ttgccatcgg gatcgtggtg aattttgata aattcaaagg gcagatgagg
     3001 ttgtagtgca tccacaacgg cacccgcgcc accgttgccg gcattcacca ccactttaat
     3061 aggtttgagg gccttggtat ccacataagt gagcagatgc tccacatagg cgccgcgcat
     3121 gtcccgctgc tcaaccaggc ccttgcgcgc cgaggcttta aactggccgg attcggcaag
     3181 ggctttaatg tccaataatc ccgtgtcgcc ggatatgggt ttagaacctt cccgcaccag
     3241 cttcatgccg ttgtaatcca tggggttgtg gctggccgtc acgcaaatgc cgccgtctac
     3301 ctccagtgca aaggtagcga agtagacctc ctcagtgccg cactggccga tatgaatcac
     3361 atccgccccg gcgtctgtca tgccgcgcac caaggcattg gtgagggccg ggctggtgag
     3421 gcgaatatcg tggcccacca ccaggcgctt agcatttaaa tattcagcga aggcgcggcc
     3481 cactcggtag gccacgtcat cgttcagctc gtcgggaact cggccgcgta tgtcgtaggc
     3541 tttaaagcag gttatgttca ttagcctttt ttcacattgt aaatgttgtt gtacacgaag
     3601 gaggtggcat ccacaaaacc gtccacgctg ccgcagtcaa aacgcttacc tttgaattta
     3661 taaccgatca cacagccttt ttgcgcttgg gttaacagcg cgtcggtgag ctgaatttca
     3721 ccgcctttac caggcttggt ctgctcgata atttcgaaaa tatccggcgt taaaatatag
     3781 cggccgataa tagcgaggtt gctgggggca tcttcttttt tcggtttttc caccatggtg
     3841 ttgatttgga aaataccgtt tttaatctgc tcgccgccga tc
//
LOCUS       DAOQGS010000398         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13068_length_3874_cov_4.892380, whole genome shotgun sequence.
ACCESSION   DAOQGS010000398 DAOQGS010000000
VERSION     DAOQGS010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13068_length_3874_cov_4.892380"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..898)
                     /locus_tag="PKZ52_13555"
     CDS             complement(239..898)
                     /locus_tag="PKZ52_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60844.1"
                     /translation="MAKAVDKRYFIYPVHGKACSATSGTAMSVILGPEAIKSTNYGSG
                     TDSTSISSYMKPLNTKDYTIIGTGPKGLWVAGHLLNDNLGGSGTDAENLTPLTQTANK
                     QHSGYEGKIKAGLDASRQLMEGYASIPFIVGIKYEVAVSSATFGTFAPYDKAPSHLSI
                     KTTLVKADKKTFDIDVLGVNDWTGIPASIANYLKAAVFATDIHNDDSHLLNCANPNCM
                     H"
     gene            complement(1166..1549)
                     /locus_tag="PKZ52_13560"
     CDS             complement(1166..1549)
                     /locus_tag="PKZ52_13560"
                     /inference="COORDINATES: protein motif:HMM:NF028005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS amidase immunity protein Tai4 family
                     protein"
                     /protein_id="HNG60845.1"
                     /translation="MATKIKAVLYIFVAAGFLATLSCSHIKTSADSKINKNKKALKNW
                     TLSYCLASALEPGPAKQDALNTAGAYLEAGNQPIEAYEAATLLINNYLAKNYTGIRPG
                     SFNTKKCIDLYESSELDSLTRKYSN"
     gene            complement(1534..2022)
                     /locus_tag="PKZ52_13565"
     CDS             complement(1534..2022)
                     /locus_tag="PKZ52_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system amidase effector
                     protein Tae4"
                     /protein_id="HNG60846.1"
                     /translation="MARPLFSKSWNLFLEVNADVKSVGDKIGGKVKFNIDNGIFTNAC
                     PIRMSYVLNYSGTSIPKPGGKYNVVSGGDAKWYMYRVAEMMTFLSATFGAPNFTAKKP
                     KIADFANKRGILLIRGSGWSDAAGHVTLFDGKSCSDACHLTGDPLNGAFTPDEASIWL
                     LK"
     gene            complement(2121..2642)
                     /locus_tag="PKZ52_13570"
     CDS             complement(2121..2642)
                     /locus_tag="PKZ52_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNG60847.1"
                     /translation="MRYRRNNVPGGTYFFTVNLLQRDKRLLVDHIEGLRQAIAQVKRR
                     RPFIIDAWVVLPDHMHAVWTLPAGDVDYSGRWREIKKAFCKQLLPIETLSSIRKAKGE
                     RGIWQRRFWEHTIKNDADYRHHIHYTYYNPVKHGLVTQVKHWPYSSFHRDVRLGLFDI
                     NWCGKPIDVVGVD"
     gene            complement(2836..3156)
                     /locus_tag="PKZ52_13575"
     CDS             complement(2836..3156)
                     /locus_tag="PKZ52_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60848.1"
                     /translation="MIKFERYRHIHDLFVHYANQENRSDILDLLNNGIASLHDAENLC
                     RFTWKIAELVNTDEENKTLVLGSTDNTDMLADLSYEISKLMRTTGYEAIWQRISNEEM
                     GEPD"
     gene            complement(3153..3500)
                     /locus_tag="PKZ52_13580"
     CDS             complement(3153..3500)
                     /locus_tag="PKZ52_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60849.1"
                     /translation="MGIRICDYQFNFDKTWILPNPQMGLSFSSTWDNLQFVYGMQTKK
                     AKNKPIDVYWILSAADIPPGLEFVEDMSQPGKGHYFLAVTVKEGMSVETLAGCLERVA
                     YRMSVIKNGGSLL"
BASE COUNT          957 a          873 c          896 g         1148 t
ORIGIN      
        1 aatctacgat tttttggggg gctttcttaa gaaagattaa tttttcagag gttccttagg
       61 tttcaagcta ttaaattttt gggtggccca gtgttgtgtt gtttagctta aatagtgcca
      121 ctagccgtgc gcaaaataat tgggcagcca aaatttatac ggcaactaag ttttgcgccg
      181 ctattttgat tgaggcagga acgcggcaca ctattcgtgt gtgccaatct atcccttgtt
      241 aatgcatgca attgggattg gcgcagttta ataagtggct gtcgtcgttg tgaatgtcgg
      301 tcgcaaatac agccgcttta agatagttgg caatgctcgc tggaatgccc gtccaatcat
      361 ttacacctaa cacatcaatg tcgaaggttt ttttatccgc tttaactaat gtggttttaa
      421 tggataagtg tgaaggcgct ttatcgtagg gggcaaatgt tccgaatgtg gcgcttgaga
      481 ccgcaacttc atatttaatc cctacgataa atggaatgct tgcataacct tccataagtt
      541 ggcgagaggc atctaagcct gcttttatct tcccttcata tccggaatgc tgtttgttag
      601 ccgtctgggt tagtggtgta aggttttctg catcagtccc tgagccgccc aagttatcgt
      661 taagcaagtg ccccgctacc caaaggccct ttgggccggt gcctattatt gtatagtctt
      721 tggtatttaa cggtttcatg tatgagctaa tgctggtgga gtctgtgcca gaaccgtagt
      781 ttgtgctttt tattgcttcc ggccctaata tcacactcat tgccgtgccg gatgtagcgc
      841 tgcaggcttt gccgtggacg ggatatataa agtaacgctt atcaactgct ttggccatga
      901 ggctaactcc aaaaatggtg gggtgcttgg tggctttgta tccctccttg caaaatccat
      961 tagcagccgc ccgttcgtgt ataaagtagg tcggaaaagc gtagcgcctt ccgacgtgaa
     1021 taattccgag aagctgcgaa gttaaaccct gcaatgtact cgcggctaga tgtcggaagg
     1081 cgcctgcggc tttgcgacct acgtaagccc aaaatgattc cgggttgcgc ggttacggat
     1141 tgtgcggctt gtacaatgaa tgtaattaat tgctgtattt cctggtaagg ctatcaagtt
     1201 cggaactctc atataggtca atacattttt tggtattaaa actacctggc cgaatacctg
     1261 tgtagttttt tgccaaataa ttatttataa gcaaggttgc cgcctcgtat gcctctatgg
     1321 gttgattgcc cgcttctaaa taggcacctg ctgtgtttaa agcatcttgc ttagctgggc
     1381 ctggttcgag ggcgctggca agacaatagc ttaatgtcca gttcttaaga gcctttttat
     1441 ttttgtttat tttggagtcg gctgaggttt ttatatggga gcaagataga gttgccaaaa
     1501 atccagccgc aacaaaaatg tacaataccg cttttatttt agtagccata tggatgcctc
     1561 atctggggta aacgcgccat tgagcggatc tcctgtaaga tggcatgcgt ctgaacacga
     1621 tttgccatca aataaggtta cgtgcccagc ggcgtcgctc cacccagatc cgcgtatgag
     1681 taatatgcct cgtttgtttg cgaaatcagc tattttgggc tttttagctg taaaatttgg
     1741 ggcgccgaag gtggccgata gaaaagtcat catttcggcc actctgtaca tataccattt
     1801 cgcatcccct ccgcttacga cattatattt tccccctggc ttggggattg atgtgcctga
     1861 ataatttaaa acgtaactca tgcggattgg gcatgcattg gtgaaaatgc cattatcgat
     1921 attaaatttt actttccctc caattttgtc gcccactgat tttacgtctg catttacctc
     1981 tagaaaaagg ttccaggatt tgctaaataa tggtcgtgcc ataaagtaac tccaattttt
     2041 ctaagtgtca gcagtaggtt ggttggaaaa gcgaatcgcc ttccgacccg cataaatgat
     2101 ttcgggttgc atgttttaag tcaatctacg ccgacgacat caattggttt tccgcaccag
     2161 ttaatatcaa aaagccccag gcgcacatcg cgatgaaacg aagaataggg ccaatgttta
     2221 acctgggtta ctaggccgtg ttttaccgga ttgtaataag tgtaatggat atggtggcga
     2281 taatccgcgt catttttaat tgtgtgctcc cagaacctgc gctgccaaat gccgcgctcg
     2341 cctttggctt tgcgtatgga cgatagagtc tcaataggca gcagttgttt gcaaaaagcc
     2401 tttttaattt ccctccagcg gccagaataa tcaacgtccc cggcaggcaa tgtccacacc
     2461 gcgtgcatat gatctggcaa taccacccag gcatcgataa taaacgggcg gcgcctttta
     2521 acttgcgcga tggcctgccg caacccctca atatgatcca ccaataatcg cttatcccgt
     2581 tgcaataaat tcacagtaaa aaaatacgtg ccacccggta cattattcct gcgataacgc
     2641 ataagtcccc ctggtaaaat ccttgccaat ttgtgtatta ggtaggtcgg aaaagcgaag
     2701 cgccttccga cgtgaatatg cattcgagac cgcgatgtta aaccgtgtat gtgctcgcgg
     2761 atagatgtcg gaaggcgcct gcggcttttc cgacctacgc aagtccaaaa atgatttcgg
     2821 gtttcgcgtt aaaagtcaat ccggctcacc catctcttcg ttagatattc gttgccaaat
     2881 agcttcatac ccggttgtgc gcatgagttt cgatatctcg tagcttaaat cagcgagcat
     2941 gtcagtattg tctgtactgc ctaacactag agttttgttt tcctcatcag tatttaccag
     3001 ctccgctatt ttccaggtga aacgacaaag gttctcggca tcatgaaggc ttgcgatgcc
     3061 gttatttaaa agatccaaaa tgtcagagcg attctcttga ttggcatagt gcacaaataa
     3121 atcgtgaata tggcggtagc gttcaaattt aatcacagta atgaacctcc atttttaata
     3181 acgctcatgc ggtaggcaac gcgttcaagg cagcctgcga gtgtctcgac gctcatgcct
     3241 tcttttacag taacggccag aaaataatgc cccttgcctg gctggctcat atcttcaaca
     3301 aactcaagcc ccggtggaat gtctgctgct gacaaaatcc aatagacgtc aataggctta
     3361 tttttggcct tcttggtttg catgccgtac acaaattgta agttatccca agtgctggaa
     3421 aacgatagcc ccatttgggg gttgggcagt atccaggttt tatcgaagtt aaattgataa
     3481 tcgcatatac gaatacccat tatagtccgg tatgcgccct catttcttgg ttcgtataga
     3541 gtgggatatt tgccgcgaat aatcggccgg gcccgtagtt gccgtatttc ggcgacaatt
     3601 ttttgcaagt cgcttaagca tttataatgt ttaatctacg tcataatcca ccatgagtgt
     3661 taaattagtt ccgtgtgtag gtcggaaaag cgcagcgcct tccgacgtga ttatgcattc
     3721 gagaccgcga tgttaaaccg tgcaatgtgc tcgcggatag atgtcggaag gcgcctccgg
     3781 cttttccgac ctacgcaagt ccgaaaatta ttccgggtta cgcgtttttc cgatctgccc
     3841 aaggttaaaa accaatccag gttacgcgtt acgc
//
LOCUS       DAOQGS010000399         3862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13128_length_3862_cov_4.491463, whole genome shotgun sequence.
ACCESSION   DAOQGS010000399 DAOQGS010000000
VERSION     DAOQGS010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13128_length_3862_cov_4.491463"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..2055
                     /locus_tag="PKZ52_13585"
     CDS             298..2055
                     /locus_tag="PKZ52_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HNG60850.1"
                     /translation="MKCTISDYLAPLCLLSLLSLAACTAETNNASSSSSSSSSSSGKT
                     GSSSSSSSSSSSSSSSSSSSSNSSSSSSSSSSSSSSSSTSSTSSSSGFPLVEARNPNI
                     WADVPDMSVVRVGDTYYMSSTTMHMNPGVPIMKSTDLINWEIINYAHKALDANNNALN
                     MNGANAYGKGTWASSINYKNGTFYVTSFSYTTNKTYIWRTTNIEGGAWTQTTLPNLYH
                     DASLLLDDDGRAYLAYGQDDIRLIELNADLSGIKSGGVNQQIIAKASSVAGSSFILKA
                     EGTLIQKINGWYYISNICWPSGKSRTQILHRARSITGPYEGRVVFQDQGIAQGEYVST
                     PDGKWYLYAFRDSGAVGRIPWLVPVQWQNDWPVAGVNGKAPQTLGFNVDDKRLRGIIR
                     SDEFTNTNLDLAWQWNHNPDATGWSLAARPGFMRLTTTRTAASFVDARNSLTQRMFGP
                     TSTGTIALDVSGMKDGDVAGLGALQSKYGFVGVTQTGSNRALVMVNAASGSAREVERV
                     SLTNNRVYLRINGNFKNQADDATFYYSLDGTTWRQIGDTLQMSYELTHFMGYRFALFN
                     YATKTAGGFVDFDYFRVSE"
     gene            2277..3728
                     /locus_tag="PKZ52_13590"
     CDS             2277..3728
                     /locus_tag="PKZ52_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNG60851.1"
                     /translation="MRTLLVNCVFSAVLTLAMSGCSGTNPTASSSSSSSSSSSSSSGK
                     SSSSSSSSSSSSSSSSGKSSSSSSSSSSSSSSSSSSAGMAGRYPNYNTNPIAADAQGM
                     PSSAQTLAAKIKLGWNMGNSMEAVLKNSDGSYLKLDGIAPENSWGNASISNELMQRVK
                     QSGFDAVRLPLSWDQYADPTTAKIDPAWLNRVKTAVQLAINNGLYVLINIHWDGGWLE
                     NNVNAQSQAAVSDKQRAYWSQIATALRDFDEHLLFASANEPAVENAAEMEVLLAYHQT
                     FVDAVRATGGKNAYRVLVVQGPSTSAEKTYQLMNKLPKDSQANKLMVEVHSYTPYQFT
                     LMTEPQDWGYTAYPFYYWGSGNHSTTDTQHNPTWGEEADIDKEFNWMKEKFTSKGIPV
                     ILGEFAAMRRNEQVPAAERNLHFKSRAYWHKTVIQKALQNGLVPFYWDAGDAKGKFGS
                     GLFDRDQDTRNDASNNTLVDTETMNALKAGAGK"
BASE COUNT         1031 a          990 c          912 g          929 t
ORIGIN      
        1 tgagctgccc taaaacaagg aaatccacgc attttcgcca gttttagggc agcgacggct
       61 aaatagatca ggacagtcta tttagccgcc gggttaataa ttgcctaaag cagcatgatc
      121 aattaaccca gtgcaacacc cattatttta aaaataaaaa cgtcttaagg aatataaaga
      181 ccgtctgtag aactcatttt tattcgctcg gcggattttt atgccgggtt aatagcaggc
      241 agggctagtc actcccacac caatccatta ataaatcaca tcgcaaagag cttttttatg
      301 aagtgtacaa tcagtgatta tcttgccccc ttatgtcttt taagcctact cagcctcgcc
      361 gcttgcactg cagaaaccaa taatgccagc tccagtagtt catcctccag cagctcaagt
      421 ggcaaaaccg ggtcaagcag ctcttcatcc agtagttcat caagcagttc atcctccagt
      481 tcttcatcaa gcaattcctc gtcgagcagt tcttcctcca gttcatcgtc gagcagctcc
      541 tcaaccagta gcacttcatc cagcagcggt ttcccgctgg ttgaagcgcg caaccccaat
      601 atttgggcgg atgtgccgga tatgtcggtg gtgcgggtgg gggatactta ttacatgagc
      661 agcaccacca tgcatatgaa ccccggtgtg cccattatga agtccaccga tttaattaat
      721 tgggagatta ttaattacgc ccataaagcg ctagatgcca ataacaacgc cttaaatatg
      781 aacggcgcca acgcctacgg caaaggcact tgggccagca gtattaacta caaaaacggc
      841 acgttttacg taacgtcttt ttcctacact accaataaaa cctatatttg gcgcaccacc
      901 aatattgaag gcggcgcctg gacgcaaacc accctgccca atttatatca cgacgcctcc
      961 ttattattag atgacgacgg ccgcgcctat ttagcctacg gccaggacga cattcgttta
     1021 attgaattaa atgcggattt atccggcatt aaaagtggtg gcgtcaatca acaaattatt
     1081 gccaaagcct cgtctgtagc gggcagtagt tttatattaa aagccgaagg gacattaatt
     1141 caaaaaatta atggctggta ttacatctcc aatatctgct ggccatcagg caaaagccgc
     1201 acgcaaattc tgcatcgcgc ccgctccatt accggcccct atgaaggccg cgtggtattt
     1261 caagatcagg gcatcgctca gggcgaatat gtcagcactc ccgatggcaa atggtatttg
     1321 tacgcctttc gcgatagcgg cgcggtgggg cgtattcctt ggttagtgcc ggtgcagtgg
     1381 caaaatgatt ggccagtggc aggggtaaat ggcaaggcgc cgcaaacgct cgggtttaat
     1441 gtggacgata aacgcctgcg cggcattatt cgctccgatg aatttaccaa taccaattta
     1501 gatttagcct ggcagtggaa ccacaacccg gatgccactg gttggtccct ggccgcccgg
     1561 cccggtttta tgcgcctcac taccacgcgc acggcagcga gttttgtgga tgcgcgcaac
     1621 agcctcaccc aacgcatgtt tggccccacc agcaccggca ccattgccct tgatgtaagc
     1681 gggatgaaag acggcgatgt ggcgggcctt ggcgcattgc aatccaaata cggttttgtg
     1741 ggcgttaccc aaaccggcag caaccgcgcc ctagtgatgg tcaacgcagc ctccggttcc
     1801 gctcgtgaag ttgagcgggt gagcctcacc aacaaccggg tgtatttacg cattaatggt
     1861 aattttaaaa accaggcgga tgacgccacg ttttattaca gcctagatgg caccacctgg
     1921 cggcaaattg gcgacacact gcagatgtcc tatgagttaa cccattttat gggttatcgc
     1981 tttgcgctgt ttaattacgc caccaaaacg gcgggcggtt ttgttgattt tgattatttt
     2041 cgagtcagcg aataagccaa tttataaacc ggcggcacat gccgccgggt taataataat
     2101 caatgcatgc ggcgtttttg cggcttaaac gcgcaaacac caaatctcaa ggcgatggat
     2161 ggtgttgcca actttaccgt cagttttatt aaaaattcta cgcgcatacc tgtgcagcca
     2221 aatttagctt gatcctgcca gtgtttaagg cgcattttta ttacaggcaa ccttttatgc
     2281 gtacattatt agtcaattgt gttttcagtg cagttttaac cctcgcgatg agcggctgca
     2341 gcggaactaa tcctaccgcg tcatcaagct cgagcagttc cagttcgtcc agctccagca
     2401 gcggtaaatc ctcaagttct agctcgtcta gctctagttc ttcaagttca agcagcggca
     2461 agtcctcaag ttctagttct tcaagttcta gctcttcaag ctccagttca tcaagcgccg
     2521 gtatggctgg ccgttatccc aattacaata caaacccgat tgcagcggat gctcagggca
     2581 tgcccagcag cgcgcaaacc ctggctgcaa aaattaaatt gggctggaat atgggcaata
     2641 gcatggaggc ggtattaaaa aattccgatg gcagttattt aaaattagac ggcatagcac
     2701 cggaaaacag ctggggcaat gcgtcaattt ccaatgaatt aatgcagcgg gttaaacaaa
     2761 gtggttttga tgcggtgcgc ctacccctct cgtgggatca atacgccgat cccaccaccg
     2821 ccaaaattga cccggcgtgg cttaatcgcg ttaaaaccgc cgtgcaattg gcgattaata
     2881 atgggctgta tgtattaatt aatattcact gggatggcgg ctggctggag aataatgtca
     2941 acgcccaaag ccaggcagcg gtgagcgata aacagcgggc ctattggagc caaattgcca
     3001 cagcactgcg ggattttgac gaacatttat tatttgccag cgccaatgaa cccgccgtgg
     3061 aaaacgccgc ggaaatggaa gtgttactcg cctatcacca aacctttgtg gatgcggtgc
     3121 gcgctaccgg cggcaaaaat gcctatcgcg ttttagtggt acaggggcca tccaccagcg
     3181 cagaaaaaac ctaccaatta atgaataaac tgcccaagga cagccaagcc aataaactaa
     3241 tggttgaggt gcactcctac acgccctatc aatttaccct gatgacggag ccgcaagatt
     3301 ggggctatac ggcctaccct ttttattatt ggggcagtgg caaccattcc actaccgata
     3361 cccaacacaa ccccacttgg ggtgaagaag cagatataga taaggaattt aattggatga
     3421 aagaaaaatt caccagcaaa ggtattcccg ttattttggg cgagtttgcc gccatgcgcc
     3481 gcaacgaaca agtgcccgct gccgagcgca acctgcattt taaatcccgc gcctattggc
     3541 ataaaaccgt catccaaaaa gcgctgcaaa atggtttagt gcctttttat tgggacgcgg
     3601 gggacgccaa aggcaaattc ggctcagggt tattcgatcg cgaccaagac acccgcaatg
     3661 acgccagtaa taacaccctg gtggacacag aaaccatgaa tgctttgaaa gcaggggcag
     3721 gcaaataaag aattctgcgg agcgccgcgt cggcttattt tatagggccg cgcagcgggc
     3781 ctctaagcgt gttttatatg tgcatcgata caagtattcc gcccaaaagt ggcggaacac
     3841 ttagccagac acacaccagc at
//
LOCUS       DAOQGS010000400         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13209_length_3847_cov_4.712816, whole genome shotgun sequence.
ACCESSION   DAOQGS010000400 DAOQGS010000000
VERSION     DAOQGS010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13209_length_3847_cov_4.712816"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKZ52_13595"
     CDS             complement(<1..303)
                     /locus_tag="PKZ52_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60852.1"
                     /translation="MLSPTLRSCLEIYTINTQKRHLVMAADIHFEAPNWSRDGRFLVI
                     NSEGLLYQVPIAEPKLIPINTDFATRCNNDHGISPDGGELVISHHAEEKNNASIIYR"
     gene            809..3439
                     /locus_tag="PKZ52_13600"
     CDS             809..3439
                     /locus_tag="PKZ52_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG60853.1"
                     /translation="MKPIKPLPSVITLVIAAGLTACGGDPNASSSSSSSSSSSSGKAS
                     SSSSSSSSSSSGKTSSSSSSSSSTSSSSSSNSSSGTVRHYSDFTPNSSRGKTLYESAE
                     GGCVDCHGVDGNGNKKITVSKYPTSENLFRKINDTMPVDAPETFINQDAADLTAYLET
                     WRVKPTSCRADQPVIYGERSLKLLTSYEFRNSVQDLFPSATIPAEYLETTGDIAIGGF
                     PSHYNAVMTGAVADLYYANAEKIADWAIAKGLPFTCTDKTACAEKFINEFAYKAFRRP
                     LVDDTTGNLEISGFKELFSKAPSPQAGLRWAIGATLISPNFPYRPEQGIPAADARAKG
                     WGKTTTSSAAGDYEVVAGGTTVKGAAFSSKSTGESLTDGTHNIYTAGNIAQSFTLTDP
                     AFITIRARANDYKGAWPEMTVSLGGQVLGKVLVDKYEVQTYQYFVTGKTGSQQLTIAF
                     SNQDQGEQPYGRTGFDKDLFIVDATVSAAKKKATTSVAQSAIELAASDAYVLDPFEYA
                     AALSYMYTGSTPDETLLAAAKNGDLNSPDKVALQIDRLQASPRGQEHVRRFAALWMQT
                     NKLYGQNFIRNDKKFTDKVRDSMAKEVEENFAYIFNNNVPFAEFYTADYTFLDATLAS
                     YYSINRNDGGAGADKFVKTPTTTRGGALTTGAFMTTFAHPESTSPILRAVYAREIMLC
                     HHIAPPPALNGERAALTAKVDELKAGGALSTRDYYEKLTNDPLCAVCHASMINPLGGG
                     MEDFSEIGLPQTQQLDFGLHRNLIPVDFNGTLIGTENLNDAAQIPFSGAKALSKAIGA
                     LSASQACLIEKAYRFSTGRALSPNALDQYKSEAALSVAEVNDLSCAAEKLNKVMQDSK
                     QSPKAMFKALGLLDVIRIRK"
     gene            3474..>3847
                     /locus_tag="PKZ52_13605"
     CDS             3474..>3847
                     /locus_tag="PKZ52_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60854.1"
                     /translation="MNRDILKSERSANRERRRFLDMLTKVGVSSTLYRAMPLLAGTLA
                     SRYAQAADLPNKRIVFMYLPNGAPPGKWLPKSLTEMNIATEFYAPVASVCEFSQVDMG
                     NGGHGNTWRSMASYNDSCQNTV"
BASE COUNT         1007 a          940 c          919 g          981 t
ORIGIN      
        1 gcggtaaata atggaggcat tatttttttc ttcggcgtgg tggctaataa ctaattcgcc
       61 tccgtcgggg gagataccgt ggtcattgtt gcagcgagta gcaaaatccg tattgatcgg
      121 aattaactta ggctccgcaa tgggcacttg atataacaaa ccctcgctat taatgactaa
      181 aaagcgtccg tctcggctcc aattaggcgc ttcaaaatga atatcggccg ccataaccaa
      241 atgacgtttt tgtgtattga tcgtatagat ttctaaacaa ctgcgcaggg taggcgatag
      301 cataattatt tgctcggaaa ggtgtttatt aaatgacggc agtttagcag cttgttgcaa
      361 tacctagcag cttgttgaaa tacgtaggtg gcgacgcgct ggcaatgcag gagtaattgc
      421 cgaatgtacg cgcaggaaca gttggcatat gtcggattaa ttctggtagt attaatccga
      481 ttaatttcgg tttaattgtt tattttattt tttttagatt gacttgccaa gctgtgatcg
      541 acaccacatc ggctgagcac tatcacctta taatcatgat gactattgcc gcaggccccc
      601 ttgtgtgacg caattaaagg tgcgaacacc atcaacttgg tgatcacacc taaatagccg
      661 catacaaaca caataattgg gccgcagaat ttgggcagta gggtttatgt cttcccgggc
      721 atccattttg acaaccctaa ttcgagttga gggacgtatg aatattctca ctgctcttgc
      781 gcgcgcgtgg ctttgcgctg cgcatcgcat gaaacctata aaaccgctgc cgagcgttat
      841 taccctggtg atagccgccg gtttaactgc ttgcgggggc gaccctaatg ccagcagttc
      901 gtcgagttca tcgtccagca gttccagcgg taaagcttcg tccagttcca gctcatcctc
      961 cagcagctcc agcggtaaaa cctcatcgag ttctagctcg tccagttcta ctagctcatc
     1021 gagttcctcc aactccagca gcggcacagt gcgccattac agcgatttca cgcccaatag
     1081 cagccggggt aaaaccctct acgaaagcgc cgagggcggt tgtgtggatt gtcacggcgt
     1141 ggatggcaat gggaataaaa aaattaccgt ctctaaatac cctactagtg aaaatctgtt
     1201 ccgtaaaatt aatgacacca tgccggtgga tgcaccggaa acctttatca atcaagatgc
     1261 ggctgattta acagcctatt tagaaacctg gcgggttaaa ccgacttcct gccgagccga
     1321 tcagccggtc atttacggcg agcgctcgtt aaaattatta acctcctacg aatttcgcaa
     1381 ttctgtacag gatttattcc cctctgccac tattccagcg gaatatttag aaaccactgg
     1441 tgatattgcc ataggcggtt tccccagcca ttacaatgca gtgatgaccg gtgctgttgc
     1501 cgatttatat tacgccaatg cggaaaaaat tgccgattgg gcaattgcca aagggctgcc
     1561 gtttacctgt accgataaaa ccgcctgtgc ggaaaaattc attaatgaat ttgcctataa
     1621 agccttccgc cgccccttgg tggacgacac cactggcaat ttagaaatta gcggctttaa
     1681 agaattattt agcaaagccc ccagcccaca agctggtttg cgctgggcga ttggggcgac
     1741 tttaatttcc cccaattttc cctatagacc cgaacaggga attcccgccg ctgatgcgcg
     1801 ggctaagggc tggggtaaaa ccactacgag ttccgccgct ggtgattatg aagtggtagc
     1861 aggtggcacc acggtaaaag gcgctgcatt tagcagtaaa agcaccggtg agagcctcac
     1921 cgatggcacc cataatattt atacggcagg taatatagcc cagagtttta ccctgaccga
     1981 tcccgccttt attaccattc gcgcccgcgc caatgattat aagggggcct ggccggaaat
     2041 gacggtgagt ttaggcggcc aggtgttagg caaagtattg gtggataaat acgaggtgca
     2101 aacctaccaa tattttgtca caggtaaaac tggttcccaa caacttacga ttgcttttag
     2161 caaccaggat caaggtgaac agccctacgg gcgcacaggt tttgataaag atttatttat
     2221 tgtcgatgcc actgtttccg ccgcgaagaa aaaagccaca acctctgttg cacaaagtgc
     2281 aattgagttg gcggcaagtg atgcctatgt gttagaccca tttgaatatg ccgcagcgtt
     2341 aagttacatg tacaccggca gcacacccga tgaaacccta ttggcggctg ctaaaaatgg
     2401 cgatttaaat tcaccggata aggtcgccct gcaaatcgac cgcttgcagg cttcgccgcg
     2461 aggccaagag catgtgcgcc gttttgcggc cctgtggatg caaactaata aactgtacgg
     2521 gcagaatttt atccgcaacg ataaaaaatt caccgataag gtcagagatt ccatggccaa
     2581 agaggtggaa gaaaattttg cttatatctt taacaacaac gtgccatttg cagaatttta
     2641 taccgctgat tacacctttt tggatgcaac ccttgccagt tactacagca ttaatcgcaa
     2701 tgatggcggc gcaggtgcgg ataaatttgt gaaaactccc accactaccc gtggcggtgc
     2761 tttaaccact ggcgcattta tgactacctt tgcccacccg gaatccacct cgcctatttt
     2821 gcgtgcggta tatgcccgtg aaattatgct ctgccaccac atagcgccac cgccagcgct
     2881 gaatggtgag cgcgctgctt taaccgctaa agtggatgag ttaaaagccg gtggcgcact
     2941 ctccacacgg gattattacg aaaaattaac caacgatccg ctctgtgcgg tgtgccacgc
     3001 cagcatgatt aatcctttag gtggcggcat ggaagatttt agtgaaattg gtttgccgca
     3061 aactcagcaa ttagattttg gtttgcaccg caatttaatt ccggtggatt ttaacggcac
     3121 cctaatcggc acggaaaacc ttaacgacgc agcgcaaatt ccctttagtg gtgccaaagc
     3181 gctttccaaa gccataggtg cattatcggc cagccaagcc tgcttaattg aaaaagccta
     3241 tcgctttagc accgggcggg cgctatcgcc caatgcgttg gatcaatata aatcagaagc
     3301 ggcgctgtca gttgccgaag tcaatgattt aagttgtgcg gcggaaaaac tcaataaagt
     3361 catgcaggac tccaagcaga gccctaaagc catgttcaag gctttaggtt tgttggatgt
     3421 cattcgcatc cgtaaataat gctgctgcgc acctgctaag atgaggatta gatatgaacc
     3481 gcgatatttt aaaatctgaa cgcagcgcca accgcgagcg ccgccgcttt ttagatatgc
     3541 tcaccaaggt gggtgtttca tccaccttat accgcgctat gccactgctg gccggtacat
     3601 tggccagccg ttatgcacaa gcggcagacc tgcccaacaa acgcattgta tttatgtatt
     3661 tacccaatgg cgcgccgccg ggtaaatggt tgccaaaatc cttaacggaa atgaacattg
     3721 ccactgaatt ttatgcaccg gtggccagtg tatgtgagtt tagccaggtg gatatgggca
     3781 acggcggtca cggtaatacc tggcgttcca tggcttccta taatgattct tgccaaaata
     3841 ccgtgga
//
LOCUS       DAOQGS010000401         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13216_length_3846_cov_3.681351, whole genome shotgun sequence.
ACCESSION   DAOQGS010000401 DAOQGS010000000
VERSION     DAOQGS010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13216_length_3846_cov_3.681351"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKZ52_13610"
     CDS             <1..307
                     /locus_tag="PKZ52_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNG60855.1"
                     /translation="QHTEIYAKPIRALGQEGDVLLVISASGNAGNLLQAVTTAHERGL
                     QVIALTGGDGGDIASLLDVQDIEICVQVDSRTRIHEIHLLCLYSLCDLIDHTLFGMD"
     gene            328..906
                     /locus_tag="PKZ52_13615"
     CDS             328..906
                     /locus_tag="PKZ52_13615"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNG60856.1"
                     /translation="MTLLRSLALVLPCVLLLTNCAETPKKEDFNPPPPPGKRSLGEFV
                     DDQKIESAVYANINNSSLDFTETHIRVNSFKGTVLLTGQVPDEELKTEAGRAASETQG
                     VGPGRVHNEIQLASNTTFFTRSNDSGLEAKIRVKLLANREFQGSKVKIVVEDRVVFIM
                     GQLTRKEGQLATEIVASTDGIEKVVRVIDFIN"
     gene            complement(1062..2582)
                     /locus_tag="PKZ52_13620"
     CDS             complement(1062..2582)
                     /locus_tag="PKZ52_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HNG60857.1"
                     /translation="MNLKQENRIRKIVIAGGGSAGWMCAAALVRVFGDSDISIVLVES
                     NEIGTVGVGEATIPHMAAFNEMLKISEPEFMRKTQGTFKLGIEFVNWRELGHSYIHPF
                     GTYGKEIDSMPFHQYWLKGMQQGWAPKLEEFALASTAARQGRFSRPINIPGSPLSEIN
                     YAYHFDASLYAKYLSEYAQKRGVVHVEGMIEQIPLDPDTGFIQSLVLKSGQVIEGDLF
                     IDCSGFRSLLLGKTLGIEYEDWSDLLPCDRAVAVPCERIEPLLPYTRSTAHSAGWQWR
                     IPLQHRTGNGHVFSSRYMSVDEATAILLSNLDGPATAEPREIKFTAGRRKKIWHKNCV
                     AIGLASGFLEPLESTSLHLIQMGVGRLISLFPDKSFHADYVDKYNEKMQNDLVGIRDF
                     IVLHYWATRRNDSEFWRYCRNMAVPATLHRKVNMYRDGGHIERENLELFTEPSWLAVM
                     HGQGVENRRYHPSVDVMPGLQVREKLEAMRLKVKNSVGELPSHGEFIKRYCSAENG"
     gene            complement(2579..>3846)
                     /locus_tag="PKZ52_13625"
     CDS             complement(2579..>3846)
                     /locus_tag="PKZ52_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60858.1"
                     /translation="PETLNFIVRDLGISLTELMTGQDPGVFCLGMQLKPSSFSEISVV
                     AHVPARAQISAMPANYAVERFRRVGGMEKLEQYSLAAQMIKANAMVLPNKKGECPLGD
                     DEIGISIRLSHLRGLLIARLKTFGVMVIQKNTMVISVDSDGVQAQFIDGSRKEFDAII
                     STAPNYELFGRVQLQQTVQWVTTDRLPAPSAELTLDVRGWMIMDAVAQGKRCEIRSIG
                     WGDKAQASMHNSVGDGEIKHISAAWFGAYWQDRIVCLSDSQFFGAEFLLDPLQPVIKV
                     VQLLTHILPVQTSADLLARYFNERVQAISLACREYIIAHLIESSIENIQDLVSEQDRA
                     LYWQRKTIYQTLGEPVQPAANLISDGIWAAFWHAHSIYPQSASNLLRSFDLDETLHQA
                     RTIESNITEIIPRLPTHQYYISLLNGGGV"
BASE COUNT         1074 a         1035 c          804 g          933 t
ORIGIN      
        1 ccaacacact gaaatctacg ccaaacccat ccgcgccctg ggccaggaag gcgatgtatt
       61 gttggtcatc agcgccagcg gcaatgcggg caacttattg caagcggtta ccaccgccca
      121 cgagcggggc ctacaagtga tcgccctcac cggcggcgac ggcggggata ttgcgtcgtt
      181 attagatgtc caggacattg aaatttgtgt gcaggtggat tcgcgcacac gaattcacga
      241 aattcatttg ttatgcctat acagcctgtg cgatcttatc gaccacacat tgtttggcat
      301 ggactaaccc aacctaaaag gccccctatg acactgctgc gctccttagc cttggttctc
      361 ccctgtgtac ttttgctcac caactgcgcc gaaaccccga aaaaagaaga ctttaatccc
      421 cccccaccgc cgggcaaacg cagtctcggc gaatttgtgg acgatcaaaa aatagaatcg
      481 gccgtgtacg ccaacattaa taacagcagc ctggatttca ccgaaaccca catccgcgtc
      541 aacagtttta aaggcactgt attattaacc ggccaagtac ccgatgaaga actcaaaacc
      601 gaagccggcc gcgccgccag cgaaacccag ggcgtaggcc caggccgcgt gcacaacgaa
      661 atccaactgg ccagcaacac cacctttttc actcgcagta acgatagcgg cttggaagcc
      721 aaaatccgcg taaaactcct agccaaccgc gaatttcaag gctccaaagt taaaatcgtc
      781 gtggaagatc gcgtggtatt tatcatgggc caactcaccc gcaaagaagg gcaattggca
      841 actgaaatag tggcgagtac ggatgggatt gaaaaggtgg tgcgggtgat tgattttatt
      901 aattaaacgc ccaaccaccg ctatttaagc gcagcatttt ctgccccacc aactggcctg
      961 catagaaatt ctgccaacgt tggagaagct catacgaaaa ccgtcgcaga caaacatctg
     1021 cgacggttta actcacaaat ggagcaatga gctaaatgct atcagccatt ttctgctgag
     1081 caatagcgct tgataaattc gccatgcgaa gggagctcgc cgacagaatt tttcactttt
     1141 aaacgcatgg cctccaattt ctctcgcacc tgaagtcccg gcatcacatc tacgctcggg
     1201 tgataacgcc tgttttccac cccttgccca tgcattaccg caagccaact aggctcagta
     1261 aatagttcca aattttcccg ttcaatatgc ccgccatcac gatacatatt tactttgcga
     1321 tgcaacgtgg caggcaccgc catgttgcgg caatagcgcc aaaactctga atcattgcgg
     1381 cgtgtagccc aatagtgcag gacgataaaa tcgcgaatac ctaccaggtc gttctgcatt
     1441 ttttcgttat atttatcgac atagtctgca tgaaagctct tatctggaaa taggcttatt
     1501 agcctaccta cccccatttg aatcaggtgc aaactagtag attctaacgg ctccaaaaat
     1561 ccgctggcca accctatggc aacacagttt ttatgccaga tttttttgcg ccgccctgcc
     1621 gtaaatttta tttccctagg ttcagccgta gctgggccat ctaaattcga caggagtatt
     1681 gccgtcgctt catccaccga catatagcgg ctggaaaaga catgtccatt gccagtgcgg
     1741 tgctgcaaag gaattcgcca ttgccaacca gcagaatggg cggtagaacg ggtgtaaggc
     1801 aacaagggtt caatgcgctc acagggaacc gccacggcgc gatcacaggg caatagatcg
     1861 gaccagtctt catactcaat gcccagcgtc ttgccgagca ataaagagcg aaaaccagaa
     1921 caatcgataa ataaatcgcc ttctattacc tggccgcttt tcaacactaa ggactgaata
     1981 aatcccgtat caggatctaa aggaatctgc tcgatcatgc cttcaacatg aaccacaccg
     2041 cgtttttgcg catattcact caaatacttg gcatacaagc tggcatcaaa atgataggca
     2101 taatttattt ctgacagggg cgagcccgga atattaatag gccgcgaaaa tcgcccttgc
     2161 cgagcagcgg ttgaagctaa tgcaaactcc tctaatttag gtgcccatcc ctgctgcata
     2221 cctttcagcc aatactgatg aaatggcatt gaatcaattt ctttaccata agtgccaaat
     2281 ggatgaatat aggagtgccc aagctcccgc caattcacaa attcgatgcc caacttaaaa
     2341 gtgccctgag ttttacgcat aaactccggt tcgctaattt ttagcatttc attaaaagct
     2401 gccatgtgcg gaatagttgc ctcgcccaca ccaacagtgc caatttcatt ggattcgact
     2461 agcacaatac taatgtcgct atcaccaaat actcgcacca gggcggcagc acacatccag
     2521 cctgcactgc caccaccggc aataactatt tttcgtatgc gattttcttg ttttaaattc
     2581 acactccacc tccattaagc agcgaaatat aatattgatg ggtaggcaag cggggtatta
     2641 tttcagttat gttcgattct atagtccgcg cctggtgcaa ggtttcatct aagtcaaacg
     2701 agcgcaataa attggaagcg ctttgcggat aaatagaatg tgcatgccag aacgccgccc
     2761 aaatgccatc agagataagg tttgccgcag gctgcacagg ctcacccaat gtctgataga
     2821 tcgtttttct ctgccaatat aaagccctat cctgctcgga gactagatct tgtatatttt
     2881 ctattgagga ttcgattaag tgagcaatga tatattctcg acaggctaaa ctgattgcct
     2941 gcacacgctc attaaagtac cgcgccaaca gatcagcaga tgtttgcacg ggcaaaatat
     3001 gtgttagaag ttgcaccact ttaataacag gctgcagggg atctaataaa aattcagccc
     3061 caaagaattg gctatcagac aaacatacta ttcgatcttg ccaataggca ccaaaccaag
     3121 cagctgagat atgtttgatt tctccatcgc ccacggaatt atgcattgat gcttgtgcct
     3181 tatcgcccca accaatgctg cgaatttcgc aacgcttacc ctgggcgacg gcatccatta
     3241 tcatccaccc acgcacatct agcgtcagtt cagcgctagg agcaggcaat cgatcagttg
     3301 tcacccactg taccgtttgc tgtagttgca cgcgcccaaa cagctcataa ttcggtgcgg
     3361 tcgagataat agcatcaaat tctttacgcg aaccgtcgat aaattgcgct tgcactccat
     3421 ccgaatcaac gctaattacc atagtatttt tttgaattac catcaccccg aacgttttta
     3481 agcgagcaat taataaaccg cgcaaatggc tcagacgaat actaatgcct atttcatcat
     3541 cacccagcgg gcactcacct ttcttattcg gcaacaccat ggcgttggcc ttaatcatct
     3601 gtgcagcaag tgaatactgt tcgagctttt ccatcccacc cacccgccta aagcgctcta
     3661 cagcgtagtt ggcaggcatt gcacttattt gtgctcgcgc gggcacatgt gccacaaccg
     3721 atatttcaga aaaagaactg ggctttaatt gcattcccaa acaaaaaacc ccaggatctt
     3781 gcccggtcat taattcggtc aacgaaatcc ccaaatccct cacaataaaa tttaaggttt
     3841 caggac
//
LOCUS       DAOQGS010000402         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13280_length_3833_cov_4.796983, whole genome shotgun sequence.
ACCESSION   DAOQGS010000402 DAOQGS010000000
VERSION     DAOQGS010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13280_length_3833_cov_4.796983"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..881)
                     /locus_tag="PKZ52_13630"
     CDS             complement(66..881)
                     /locus_tag="PKZ52_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60859.1"
                     /translation="MGPAPSLQGIICEPLPGAAASNTAMILLNSGVIHRVGSCRLSVL
                     LARSVCTAANIVTVRFDFSGIGESDARRSALTADEVAVAEVIEVMNYLAQHKKIERFI
                     LYGLCSGAYASYRTALKDPRVIAIAQIDGYCYSSWKTYWHHFVPRLLSSARWASVAKR
                     LLGLKKTVSGAAVSGIEAKFFEVPNFGNFPPQAEVTEGLKQLVKRQVRFFNLFCRGEH
                     YNYADQYRDCFSSVKFGPLLELLYLPNASHILAEPEDRALVIERMTAWVKSQV"
     gene            complement(899..1672)
                     /locus_tag="PKZ52_13635"
     CDS             complement(899..1672)
                     /locus_tag="PKZ52_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNG60860.1"
                     /translation="MQTFYFGPAASSLYGAYHEPQGVPRREGIVLCYPFGQEYMRAHR
                     SFRRLAINLAAKGFAVLRFDYRGCGDSAGDTPGVTSQDWIADIHAAIQELKDIAAVQT
                     VSLMGLRLGGLLAAAAAQNQNINRLVLWDCSLSGTAYCDEISREIAAQQSRSKFIAPN
                     GDMLYNGFNLPSSLQTGLKQLDLLQMEQLKQLQWSLIVSHEHANFTALKEAYSSNPRF
                     SYTLAPAPHDWNYVDHVGGILWPAPIVAAIEQYFEGGLA"
     gene            complement(1753..3774)
                     /locus_tag="PKZ52_13640"
     CDS             complement(1753..3774)
                     /locus_tag="PKZ52_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HNG60861.1"
                     /translation="MEFPSELFNASSVQAFADSLVESARAFVAKPDATIAELASALPN
                     DRSRLAELNPPAKPVAFDSVLSQLARARAQYGQESAVLAGDGSYNLRELEVRSNELAW
                     SLKAQGIGPGSLVALVLKRGYDLPAVIIALWKLGAAYMPLDPSLPRARVESMLETAQV
                     TALLGHQRWAEDFGVHRYLSLETLEQVSFEPYPEVSLTESTRAYVIFTSGSTGVPKGV
                     EVGHGALANFLTAIGEKLNLKPGHRLLAITTLSFDISILELFLPLLAGASVRVADENL
                     VLDGFALKQQLEQSAITHLQATPTTWRMLLTAGWRAPKGFNALCGGEALPSDLAAGLI
                     QAGCTLWNLYGPTEATVWTATYKVADSAELLVSRGGAPVYLGEPLANTQLYVLDDRLQ
                     PLPLGVYGDLWIGGAGLALGYIGAPELTAERFITDAEGRRLYKSGDIARWNQSGQLEY
                     LGRSDLQVKLRGFRIELAEIESALRSHPAITDAAVVVQDLGSDQRLVAFVIYREGDEP
                     TASQLRSHLRQSLPDYMLPQQFTQVEQFPTTASGKLDRKKLAAPEEAGHTSSHAYTPP
                     TTATEKLLVEIWAGALLKEQVSIDSQFFDIGGHSLLALEVILAIEEACQVRFAPQDMW
                     VNTLEQLAAKIDASRSAGSPQPEPVPATEAGSPEQEKRSGWLGRLFGRD"
BASE COUNT          821 a         1116 c         1151 g          745 t
ORIGIN      
        1 ggccgcaacc gcttgtggca agcgcgcccc ttcgagacag aagaatgcgc tcccagagcc
       61 caggattaga cttggctttt aacccaagcg gtcatgcgct caatgaccag ggcccgatcc
      121 tccggctccg ccaaaatatg cgaagcgttg ggcaggtaaa gcagctccaa cagagggccg
      181 aatttcactg agctaaaaca gtcccggtat tgatcggcgt agttgtaatg ctcgccgcgg
      241 cagaagaggt taaaaaaccg cacttggcgt ttaaccaatt gctttaggcc ttcagtaacc
      301 tcagcctggg gcgggaaatt accgaagttt ggcacctcaa aaaacttcgc ttcaatcccc
      361 gaaacggccg caccgctgac ggtttttttc agtcccaata ggcgtttggc tacgctcgcc
      421 caacgggcgc tggaaagcag gcgaggaaca aagtggtgcc agtaggtctt ccagctggaa
      481 tagcagtagc cgtcgatctg ggcgatggcg ataaccctag ggtcttttag ggcggtgcgg
      541 taggaggcat aggcgccaga gcagagcccg tacaaaataa agcgctcgat ttttttgtgc
      601 tgagccaggt aattcattac ctcgatcact tccgccaccg ccacttcatc ggcggttagc
      661 gcgctgcggc gggcgtcgct ctcgccgata ccggagaaat caaaccgcac cgtaactata
      721 ttggcggcgg tacagacaga gcgcgccagc aataccgata aacgacagga acccacccgg
      781 tgaatcactc cagagttgag caatatcatg gcggtattgc tcgcggcagc ccctggcaga
      841 ggttcgcaga tgatgccctg taggctaggc gctgggccca tgctgagtgt tttttccctc
      901 acgctaaacc cccttcaaaa tactgttcaa tggccgccac tataggggca ggccaaagga
      961 tgccgcccac atggtccaca taattccagt cgtggggcgc aggcgccagg gtgtagctaa
     1021 agcgcgggtt gctgctgtag gcctctttaa gggcggtgaa attggcgtgt tcgtgggaga
     1081 cgattaggct ccattgcaat tgcttaagct gttccatctg caataagtcc agctgcttaa
     1141 ggcccgtttg caggctgctg ggcagattaa aaccgttgta gagcatatcg ccgttggggg
     1201 cgataaattt agagcgggac tgctgcgcag ctatctcccg gctgatctca tcgcagtagg
     1261 ctgtgccgct taaggagcaa tcccacagca ctaaacggtt gatgttctgg ttctgcgccg
     1321 ctgccgccgc cagtagcccg cccaaacgca ggcccatcag ggagacggtc tgcaccgctg
     1381 ctatatcttt aagctcttgg atggcggcgt gaatatcggc gatccagtct tggctggtca
     1441 cgccaggagt atctccagcg gaatcgccac agcctcggta gtcaaaacgc agtaccgcaa
     1501 aacccttggc cgccaggtta atggccaggc ggcgaaagct gcggtgggcg cgcatgtatt
     1561 cctggccgaa gggataacac aggactatgc cctcccggcg cggcacgccc tgaggttcgt
     1621 ggtaggcgcc atatagggat gacgctgcag ggccaaaata aaaagtttgc aatgggcctc
     1681 cttttagaaa aaacagacaa atgccgcagg tgcggcacta tagccccaag gggtcagtaa
     1741 aggaacactc acctaatcgc gcccaaacag gcggcccaac cagccggagc gtttttcctg
     1801 ctcagggctg ccagcttccg tggctgggac gggctcaggc tgggggctac cagctgaacg
     1861 gctggcgtca attttggcag ccaactgctc cagggtgttt acccacatat cttggggagc
     1921 aaaacgcacc tggcaggctt cctcaatggc caaaatcact tccaaggcga gcaaagaatg
     1981 gccgccaata tcgaagaatt ggctgtcgat actgacttgt tctttaagca gcgcaccggc
     2041 ccaaatctct accagcagct tctcggtggc agtggttggc ggtgtgtagg cgtgactgct
     2101 ggtgtggcca gcctcctcgg gagcggcgag ttttttgcgg tcgagtttgc cggaggccgt
     2161 ggtggggaac tgctccactt gggtgaactg ctgaggcaac atgtaatccg gcaggctttg
     2221 gcgcaggtgg ctgcgcagtt gggaggcagt gggctcatca ccctcgcggt agataacaaa
     2281 cgccaccagg cgctggtcgc tgcccaaatc ctgcaccacc acagccgcat cagtaatagc
     2341 cggatggctg cgcagcgcgc tttcgatttc ggccagctca atacgaaagc cccgcagttt
     2401 gacctgcaaa tcgctgcggc ctaaatactc caactggccg ctttgattcc agcgggcgat
     2461 atcgccggac ttatacaagc ggcggccctc ggcgtcggtg ataaagcgct cggcggtaag
     2521 ctcaggggca cctatataac ccagggccag gcccgcgccg ccaatccaca agtcgccata
     2581 cacccccagg ggtaagggct gcagcctgtc atccagcaca tacaactggg tgttggccag
     2641 gggctccccc aggtatacgg gcgccccgcc ccggctaaca agcagctcgg cgctgtcagc
     2701 aaccttgtaa gtggccgtcc atacggtggc ttcggtgggg ccatagaggt tccaaagggt
     2761 gcagcccgct tggataagcc cggcggccaa atcgctcggc agcgcctcgc cgccgcatag
     2821 cgcattaaag cccttgggcg cccgccaacc ggcggtaagc aacatacgcc aagtagtagg
     2881 ggttgcctgc aggtgggtga tggcggactg ctccagctgc tgctttaaag caaagccgtc
     2941 caacaccagg ttttcatcgg ccaccctgac actggcgcca gcaagcagcg gtaaaaacag
     3001 ctccagaata gaaatatcaa aactcaaggt ggtaatggca agcaggcggt ggccgggctt
     3061 taggttcagt ttttcaccta tggcggttaa gaaattcgcc aaagcgccgt ggcccacttc
     3121 cacaccctta ggcaccccgg tggagccaga ggtgaagatc acataggcgc gggtggattc
     3181 ggtaaggctc acctctgggt agggctcgaa ggagacctgc tcaagggttt caaggctcag
     3241 gtagcggtgc acgccgaagt cctccgccca gcgctgatgg cccagcaggg cggtaacctg
     3301 agcggtctcc agcatggatt ctacccgcgc cctgggcagg cttgggtcca ggggcatgta
     3361 ggcggcaccg agtttccaca gggcgataat cactgcaggc aagtcatagc cgcgcttgag
     3421 caccagagcg accagactgc ctgggcctat gccttgcgcc ttaaggctcc aggccagctc
     3481 gttgctgcgc acttctaact cgcgcaagtt gtagctgcca tcccccgcca gcacagcact
     3541 ctcctggcca tactgggcgc gggcgcgggc taactggctg agcacactgt caaaggccac
     3601 tggcttggcg ggtgggttga gctcggccag gcggctgcgg tcatttggca aggcgctggc
     3661 gagttcggcg atggtggcat cgggcttggc aacaaaggcg cgggcggatt ccaccaggct
     3721 atcggcaaag gcctgcacgc tactggcgtt gaagagttcc gaggggaatt ccatgccgcc
     3781 gtccctactg ggccaagcgc ttcggcgtgg cgcccttcct gcccatgtct cgc
//
LOCUS       DAOQGS010000403         3829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13305_length_3829_cov_3.754902, whole genome shotgun sequence.
ACCESSION   DAOQGS010000403 DAOQGS010000000
VERSION     DAOQGS010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13305_length_3829_cov_3.754902"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3829)
                     /locus_tag="PKZ52_13645"
     CDS             complement(<1..>3829)
                     /locus_tag="PKZ52_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HNG60862.1"
                     /translation="HGQHTLSATQINNTQGLIYSDAQTLTVTASTVDNTAGRWQHSGQ
                     TLIVNGLDEWINKANADTAALLQTDGTLVIQGFNRLLNDGQVGEAPSTIRARSAQLQG
                     KRVENLNGAQLALVVTEKADGNASGIPQALASLPPVKSTLKLDQLVNQGRIFTQASLN
                     LSDANLLNTGGEFSSFSDLTLNAGDLGALGGKLWADGILTLTSTGDFTFANQAQLDAG
                     RGIVWDVKGAFTNQGTLASGTNVTLKANAFTNTAAADLSALGTLALEVAQALTNQGRI
                     RGSSDTQVQANSLENNGLISAQQDLKVTAGASLTNTNTLFATGNLIALAPELFNTSTP
                     TTTASIWAGGKLILAGGQAESGRVPRVGQQLINTSGSIQTYQAGDIELWFNQVVNQAE
                     PFELKPETINVKQWFTTGSGKPADAINDGEFDITISELMGLMDRSGNQLPYIEHNLYV
                     FQPAKVVRQPRINSGGALLIQTLNDPKSAQAECSGTPCKPALIRNEGGSLSAVGNLTL
                     EAGYDPDTVSGTVLGHGSRLDNSNLYLPGETTQESNQFINVIPDPGVAYTDIARFSNM
                     GTFTSGPFFTGTQAVEKAATLHIGTLIAGGELTAHVENVENGEKAIYQAAGTGPAITG
                     DAPPPMDFSQGAPFSRVALSNADSTQVKVTERTQLDPNQASSDGLGSTGDTDITPPSQ
                     PQDEQTQAREARIYNPLQNLVLPNNGYLYRVDIKGSSPYVVQAADLLKPPTDTPNTEK
                     PGTPPTSSDYFFSQLGYDPEQIGRRLGDSFYETELVRQAVMETRGVRFVTDPRTGLAI
                     DSDEAQYRYLLDNALEAQKDLQLAVGTALTPEQVRALTQDIVWLERHEMAGQEVWVPV
                     YYATGVDPTQMAKGAVMAGQSVRVVSEELLNRGDIRGLSLVDVSNQKSQNFGAIGSLD
                     RLIVKGQNLDNKGLMLGKTTQVSGFDIDNSGQMIARETLDVTADHELSNRGELAGADV
                     NAKSTGGDVINQGQINASSNVDLDAGRDILLKPGQGAGITTGRNLTAKAARDFNLEGQ
                     TINTQGDATLSATRDVTITPTTERSLTSGGNTSTEAVTHTATKITTGGSLSIDAGRDL
                     GITATKIDTGKDLTLRGERDVTLTSAADSTHTEGQGKNWHTIETDVKQVTTNISAGGN
                     IDANAGQDLNLVAVKGKADGDIHLSAKRDTNLLGAVDSEYDYSYEKKKKSLGRSKTKE
                     SESLTETAVATELSAGGNLLLNTKLGSKGEILKETGQRINTEGAKLSAGDPEASGETQ
                     GTIAAYAADGIILA"
BASE COUNT          665 a         1001 c         1169 g          994 t
ORIGIN      
        1 cggccaagat gatgccatca gcggcataag cggcaatggt gccctgggtt tcgcctgagg
       61 cttcgggatc gccagcactg agcttggcgc cttcggtatt aatgcgctgg ccagtttcct
      121 tgaggatttc gcctttgctg ccgagtttgg tgttcagcaa cagattgccg ccagcgctta
      181 attccgtcgc gaccgccgtt tctgtaaggc tttcactttc tttagtttta ctgcggccca
      241 ggcttttttt cttcttctcg tagctgtagt cgtattcaga atccacggca cccaataggt
      301 tggtgtcgcg cttggcgctg aggtgtatat cgccatccgc tttgcctttg acggccacta
      361 ggttgaggtc ttgcccggca ttggcgtcga tgttgccccc ggcgctgatg ttggtggtga
      421 cttgcttaac gtcggtttca atggtgtgcc agtttttgcc ttggccttca gtatgagtgc
      481 tatcggcggc gctggtgagg gtgacgtcgc gctcacctcg aagggtgagg tctttgccgg
      541 tatcgatttt ggtggcggtt atgccaaggt cgcggcctgc atcgatactt agggagccgc
      601 cagtggtgat cttggtggcg gtgtgggtga cggcttcagt gctggtatta ccgccgctgg
      661 tgagtgagcg ctcagtggta ggggtgatcg ttacatcgcg ggtagcgctg agggtggcgt
      721 cgccctgggt gttgatggtt tgaccttcga ggttgaagtc gcgcgccgcc tttgcagtga
      781 ggttgcggcc agtggtgatg ccagccccct gccctggttt aagcagaata tcccgccctg
      841 catcgagatc aacattactg ctggcgttaa tctggccctg gttgatgaca tccccgccgg
      901 tacttttggc gtttacatcg gctccggcca gctcaccccg gttgctcagt tcatggtcag
      961 cggtaacgtc caaggtctcg cgggcgatca tctggccgct gttgtcgata tcaaagccgc
     1021 tcacctgggt ggttttaccc agcatcaagc ccttgttatc gaggttttgg cctttgacga
     1081 ttaaacgatc caggctgcca atcgcaccaa aattttggct tttttggttg ctcacatcta
     1141 ccaggctcag accgcgaata tcccctcggt tgagtaattc ttcactgacc acgcggacag
     1201 attgccctgc catgacggct cctttggcca tctgggttgg gtccacgccg gtggcgtaat
     1261 acaccggcac ccaaacctct tgcccagcca tttcgtggcg ctccaaccag acgatatctt
     1321 gggttaaggc gcgcacttgt tcgggtgtta gggcggtgcc caccgccagt tgcaggtctt
     1381 tttgcgcttc gagggcgtta tccagtaggt agcggtattg ggcttcgtcc gaatcgatgg
     1441 ccaggcccgt gcgcgggtcc gttacaaaac gcacaccgcg cgtttccatc accgcctggc
     1501 gcaccagctc ggtttcataa aagctgtcgc ctaagcgacg gccgatctgt tctgggtcgt
     1561 agcccagttg cgagaaaaag taatcgctgc tggtgggcgg cgtgccgggt ttctctgtgt
     1621 tgggtgtatc tgtgggtggt ttgagcaggt cggcggcctg tactacatag ggtgagctgc
     1681 ccttgatgtc cacccggtac agatagccat tgttgggtaa taccaggttt tgcagcgggt
     1741 tgtagatgcg tgcttcgcgg gcctgggttt gctcatcttg gggttgtgag ggcggcgtta
     1801 tgtcggtgtc gcctgtgctg cctaaaccgt ctgagctggc ctggttgggg tcaagttgag
     1861 tgcgctcggt gaccttaact tgggtggagt cagcgttgct cagggcaacc cggctaaacg
     1921 gtgcaccctg actgaagtcc atcggcggcg gcgcatcgcc ggtgatggcc gggccggtcc
     1981 cagcggcttg gtaaatcgct ttctcgccgt tttcgacgtt ctcaacatga gcggtgagtt
     2041 cgccaccggc gatgagggtg ccgatgtgca aggtggcggc tttttcgacg gcttgagtcc
     2101 ctgtgaaaaa aggcccgctc gtaaaagtgc ccatgttgga aaagcgcgcg atgtctgtgt
     2161 acgccacacc aggatctgga atgacgttga tgaattgatt tgattcttgg gtggtttcac
     2221 ccggtagata gaggttggaa ttgtctaggc gactgccgtg gcccagcaca gtgccggaga
     2281 cggtatcggg atcataaccc gcctcgagtg tgagattgcc caccgcactc aagctaccgc
     2341 cttcattgcg aatcaacgct ggtttacaag gggtgccaga acattccgcc tgtgcggatt
     2401 tagggtcgtt tagggtttgg atcaataaag cgccgccaga attaatacgc ggctggcgaa
     2461 ccaccttggc gggctgaaat acgtacaggt tgtgttcaat gtagggtagt tggttaccac
     2521 tgcggtccat taaacccatc agctcgctga tcgtgatatc gaactcccca tcgtttatgg
     2581 cgtcggcagg cttgcctgag cccgtggtga accactgttt cacgttaatc gtctcgggct
     2641 ttagctcaaa gggctcagcc tggttaacaa cctggttaaa ccacagttca atatcacccg
     2701 cctggtaggt ttgaatactg cccgaggtat tgatgagctg ctggcccacc cgtggcaccc
     2761 ggccgctctc ggcttgacca cccgcaagaa ttaacttgcc cccggcccag atgctcgccg
     2821 tggttgtggg tgtgctggta ttgaacaact ccggtgccag ggcgatgaga ttcccagtgg
     2881 caaacaaggt attggtgttg gtaaggctgg cccctgcagt cacttttaag tcttgttgag
     2941 ccgagattag gccgttgttt tcaaggctgt tggcctgtac ctgggtatca cttgagcccc
     3001 gtatgcggcc ttggttcgtt aaagcctgag ccacctcaag ggcgagtgtg cccagtgcgg
     3061 acagatctgc agccgctgta ttggtaaagg cgttggcctt cagagttaca ttcgtgccgc
     3121 tggcgagagt gccttggttg gtaaaggctc ccttaacatc ccacacaatg cccctgcctg
     3181 catccaattg cgcttggttg gcgaaagtaa aatcgccagt actggtaagg gttaggatgc
     3241 catcggccca gagtttgcca cccagtgcgc caagatcccc ggcgtttagg gtgaggtcgc
     3301 tgaagctaga aaattcgccg ccagtattga gtaggtttgc gtcactaaga tttaggctgg
     3361 cctgagtgaa tatgcggcct tggttgacca gctgatccaa cttcaacgtg gatttaacgg
     3421 gcggcagact cgccagggct tggggtatgc ctgaggcgtt accgtcggct ttttcagtga
     3481 ccactagtgc cagctgagcg ccgtttaggt tctcaacacg tttcccctgt aattgcgcac
     3541 tgcgggcgcg gatggtgctg ggcgcctctc ctacctggcc gtcgttgagg agccggttaa
     3601 acccctgaat aacaagagtg ccgtcggtct gcaacagcgc ggctgtatcg gcattggcct
     3661 tgttaatcca ttcatccaag ccgttaacga ttagcgtctg cccgctgtgc tgccagcggc
     3721 cagcggtgtt atctacggtg ctcgcggtga cggttagggt ttgggcatcg ctataaatga
     3781 gcccttgggt attgttgatt tgggttgcgc tgagggtgtg ttggccgtg
//
LOCUS       DAOQGS010000404         3820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13358_length_3820_cov_4.609296, whole genome shotgun sequence.
ACCESSION   DAOQGS010000404 DAOQGS010000000
VERSION     DAOQGS010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13358_length_3820_cov_4.609296"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKZ52_13650"
     CDS             complement(<1..383)
                     /locus_tag="PKZ52_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60863.1"
                     /translation="MDIAALFDAGWTVIPRAFKQASALGEFAESLRQNYRPAQIGRSQ
                     SARLEQSVRSDKILWLDNLENPAVNEYLQAMEAIRQEVNRRCFLGLFDYECHFARFEA
                     GDFYKTHLDAFADEHNPRGNRKLSSV"
     gene            complement(398..1246)
                     /locus_tag="PKZ52_13655"
     CDS             complement(398..1246)
                     /locus_tag="PKZ52_13655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNG60864.1"
                     /translation="MSEICVSVVICTRNRAQYLLECLRSFEKIHTDINWQLILIDNGS
                     SDNTQAVISQFAAATSIALVVGQEPNKGLSYARNKGIELAEGEFIAFTDDDCYPETDY
                     VDKLLIRLTSDPKLGFVGGRVLLFDPEDYPVTIQTYNEPMYLNPGEFLFAGVIHGANF
                     AFRKTALVQAKGFDVRLGAGTPFPSEDVDTMAELLRLGWRGEYDPAIVVYHHHRRRSQ
                     EEVDKLFVGYDWGRGAYFAKHILKPGHRLLFAKRWFNNIRWQPARTSRREIQAALVFW
                     WRSLVG"
     gene            complement(1341..2507)
                     /locus_tag="PKZ52_13660"
     CDS             complement(1341..2507)
                     /locus_tag="PKZ52_13660"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNG60865.1"
                     /translation="MPLISVLVLDAAQRSALAVTRSLGQLPNVLITTAERANKSLAGA
                     SRFSHESLVYPCPQAQPGEFVAWMSGLLSNRSFDWVFPVTEVTSQLLLMYQAQIPNLR
                     LPFAPLETVMALADKGQLTERAQALGVPVPQSQWHANTDSLKADHLTYPVVVKPCLSK
                     IYTQNHWLATSVFVAQDAAALTRELALRPYLAEHPFMVQEFIPGSGAGVFALYNRGQP
                     VAFFAHKRLREKPPEGGISVLSESAAVTPTLERYARTLLDAVNWHGVAMVEFRETPGG
                     DFYLMEVNTRFWGSLQLAIDAGVDFPALLLRASQGEALPPQAPYKIGQKLRWLLGDLD
                     SLYIYLKRPYTFGAKLRRVIEFLSPSFSAQRYEINRWGDLAPAWRELYQYFKQR"
     gene            2618..3760
                     /locus_tag="PKZ52_13665"
     CDS             2618..3760
                     /locus_tag="PKZ52_13665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNG60866.1"
                     /translation="MPATPPSILHAIDTTGPGGAETVFLDLAQQLIIPGYRNIALIKG
                     PGWVEDQLIKRGITYFILKPHGFLSLPYYWQVLKLLREQNVRLVQANLLGSTLTFSIV
                     TLIMCIPLVATLHGQVDVNPKERFIGFKNLLMKWGVNQLVTVSEDLRRYVATRKLFPF
                     DAIKTIYNGIDPHRYTGAQTIQLREQLGLPAHSRLIGAIGNIRPAKDYANLVHAAEHV
                     IKQHSDVHFVIAGHPKKDLMDEINRLVAQLNLSSHIHFIGFLDNTPGFLAEMEIFVLS
                     SISEGFSIATLEAMASRLPVIATRCGGPEEILSDGRDGLLVAKQNPAALSEAILNTLD
                     DTALKGSLATAAHTTVQERFSISAMLKSYRHLYGPLLKQPTTEAAR"
BASE COUNT          994 a         1051 c          906 g          869 t
ORIGIN      
        1 accgacgaaa gtttgcgatt gccccgcggg ttgtgttcat cggcgaaagc atccaggtgc
       61 gttttataaa aatcccccgc ttcaaaacgg gcaaaatggc attcgtaatc aaataggcct
      121 aaaaaacaac gccgattgac ctcctggcga atggcctcca tagcctgcaa atattcgttt
      181 accgcagggt tctctaagtt atctagccac agaattttat ccgaacgcac cgactgctcc
      241 agccgcgcag actgactgcg gccgatctgg gctgggcgat aattttgcct taatgactcg
      301 gcaaattcgc ccagtgcgct cgcctgctta aacgcgcggg gaattacagt ccaaccggca
      361 tcaaacaagg ctgcaatatc cataacctac taccccctca acccacaagg gagcgccacc
      421 agaaaaccaa ggcggcttga atttcgcgcc gggaggtacg cgcaggttgc caacgaatat
      481 tattaaacca gcgtttagca aataaaagtc ggtggccagg ttttaaaatg tgtttagcga
      541 aataagcgcc gcgcccccag tcgtagccca caaataattt atccacctct tcttggctgc
      601 ggcgccgatg gtggtggtat accacgatgg cggggtcata ttcaccccgc cagcctaatc
      661 gcaataattc cgccatggtg tcaacatctt cactggggaa gggcgtgccc gcgccaaggc
      721 gcacgtcaaa acccttagcc tgcacaagag cagtttttcg aaaggcgaaa ttcgcgccgt
      781 gaattacacc ggcaaataaa aattcgcccg ggtttaaata catgggctca ttgtacgtct
      841 ggatcgtgac tggataatct tccgggtcaa ataataaaac gcgcccacca acaaaaccta
      901 gtttcggatc ggatgtgagt cgaatcaata atttatccac ataatctgtt tcgggataac
      961 aatcgtcatc agtaaaagcg atgaattccc cttcggccag ctcaataccc ttattgcgcg
     1021 cataactgag ccccttatta ggctcctgcc ccaccactag agcaattgag gtcgcagcgg
     1081 cgaattggct aattactgcc tgggtattat ccgatgagcc gttatcaatc aggatcagct
     1141 gccaattaat atcggtgtgt attttttcaa aagaccgcag acactcaagc aggtattgcg
     1201 cgcgattgcg cgtacatatc accactgaaa cgcaaatttc actcacgcta ttcacctgtt
     1261 ttatgtttaa ttcatcgcag cgagtgcttc cgttgcgatc ggttcaaaga accatgatta
     1321 cgccacgact caactgcacg ctaacgctgt ttgaaatact gatacaactc ccgccacgcg
     1381 ggggccaaat cgccccagcg attaatttca taacgctgag cgctaaaact tggcgaaaga
     1441 aattcgatta cgcgacgcag tttcgccccg aatgtgtagg ggcgcttaag ataaatatag
     1501 aggctatcca aatcccctaa cagccaacgc aatttttgcc ctattttata aggtgcttgc
     1561 ggcggtagcg cttcaccctg gcttgcgcgc agcaacaaag ccggaaagtc aacgcccgca
     1621 tcaattgcca attgcaggga tccccagaaa cgggtgttca cttccatcaa gtaaaaatct
     1681 ccgcccggcg tttcacgaaa ctccaccatg gcaacaccgt gccaatttac tgcatccaac
     1741 agcgtacgcg cgtagcgctc aagggtcggg gtaaccgcag cactttcact taaaacacta
     1801 ataccgccct ccgggggttt ttcccgcagc cgtttatggg caaaaaacgc tacaggctgc
     1861 ccccggttat aaagcgcaaa cacacccgca ccgctgccgg gaataaattc ttgcaccata
     1921 aatgggtgct cggccaagta gggccgcagg gctaattcgc gggtgagcgc ggcagcatcc
     1981 tgagcaacaa acacgctggt ggcaagccag tgattctgcg tataaatttt tgatagacag
     2041 ggtttaacca ctaccgggta ggttaaatgg tcagctttta acgaatccgt attggcatgc
     2101 cattgggact gcggaaccgg tacgcctaag gcctgcgccc gctcggtaag ctgcccctta
     2161 tccgccaggg ccataacggt ttccaacggc gcaaagggca gacgaagatt gggaatctgc
     2221 gcctgataca tcaacaacaa ctggctggtg acttcggtaa cgggaaatac ccaatcaaat
     2281 gagcggttgc tgagtagacc actcatccag gccacaaact cgccaggctg agcctgcggg
     2341 caggggtata ccaaactctc atggctaaaa cgcgaagcac ccgccagact cttattcgct
     2401 cgctccgccg tagtaattag cacattaggt agctgaccta aagagcgcgt caccgccagt
     2461 gcactgcgct gagcggcatc gaggacaagt acagaaataa gcggcatgag gtgggctcta
     2521 attatgatgg cggcatttta cgggcaatag cgccgcctgt ggtaagtgca ttgctgttta
     2581 tagtacattg gcgcactttt ataactgcgc cctcgctatg cctgccaccc ccccgagcat
     2641 ccttcacgcc atagacacca cagggccagg tggcgcagaa acggtttttt tagatctcgc
     2701 tcagcagcta ataatccccg gctaccggaa catagcgctt ataaaaggcc ctggatgggt
     2761 ggaggatcaa ttaattaagc gggggattac ctacttcatt ctcaagcccc acggcttttt
     2821 atccctaccc tattattggc aagtgttaaa gctgctgcgc gagcaaaacg tgcgcttggt
     2881 gcaggctaac ctgctcggct ccacgctgac tttttcaatt gtcacgctca ttatgtgcat
     2941 tccattggta gctacgctgc atggtcaagt ggatgttaat cctaaggagc gatttattgg
     3001 ttttaaaaat ctgctcatga aatggggcgt gaatcagttg gttaccgtga gcgaggattt
     3061 acgccgctac gtcgccaccc gcaagctgtt tccctttgac gcgattaaaa ctatttacaa
     3121 cggtatcgac cctcaccgct acaccggtgc tcaaactatc caattgcgcg aacaattggg
     3181 cctgcccgcc cacagccgct taatcggtgc catcggcaat atccgccccg ctaaggatta
     3241 cgccaacctc gtgcacgcag cggaacacgt gatcaagcaa cacagtgatg tgcattttgt
     3301 tattgccggg catcctaaaa aagacttaat ggacgaaatt aaccgcttag ttgcccaact
     3361 caatctcagt agccacatac atttcatcgg ttttttagac aatacgcctg gctttctggc
     3421 agaaatggaa atcttcgtac taagctccat cagcgaggga tttagcatcg caaccttgga
     3481 agccatggcc agccgcttgc cagtcatcgc gacccgttgt ggtggccccg aagaaattct
     3541 cagcgacggc cgcgatgggc tcttggtggc caaacagaac cccgcagccc tatctgaggc
     3601 gatattgaat accttggatg acacagcctt gaaaggctca ctcgcaactg cggcacacac
     3661 aactgtacag gagcgatttt ccataagcgc tatgttaaaa agttaccggc acctatatgg
     3721 ccccctttta aaacagccta ctaccgaggc cgcgcgatga ctcgatttag ccgaggaatc
     3781 aaaaataatt ttggctcgaa aaagggcctg gtgcgatttg
//
LOCUS       DAOQGS010000405         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13398_length_3812_cov_3.772691, whole genome shotgun sequence.
ACCESSION   DAOQGS010000405 DAOQGS010000000
VERSION     DAOQGS010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13398_length_3812_cov_3.772691"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKZ52_13670"
     CDS             <1..570
                     /locus_tag="PKZ52_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNG60867.1"
                     /translation="VQWGTQWYIFYHNRAIANERGKSDYQRSVNVDLLNFTADGGMTV
                     NAGRQGVPQLKNFNPYNLVEAETLEKESGGIEVERVGDALVVAFTDNSWIKVAKVDFE
                     TGAAGVELRLAAAADSKIEVILDDLKNAPAAVIPVKATGDLTKFATQAVKLSGLKGVH
                     DVYYRVEGRVNVDTYKFVREAGEGALKKK"
     gene            complement(1185..>3812)
                     /locus_tag="PKZ52_13675"
     CDS             complement(1185..>3812)
                     /locus_tag="PKZ52_13675"
                     /inference="COORDINATES: protein motif:HMM:NF023959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin family protein"
                     /protein_id="HNG60868.1"
                     /translation="GEGLNNQVQQSLSGAVSIQAAGDINSGRWYSANNRFEVASLGNM
                     GKTEGELAAVIGLGDAPVPLTATGNLAVEAVFNPTQLTLSYRQFSSNPIAADAIANSV
                     LGSDHSVDNYYSTFGKQSAVAFTSIAGDVGLLMNMDLKQPLLNESKSPTTARTPGLNI
                     LPSKLDVASVQGDINLNGSIILAPEKDGRLNLTAQGNIVANSELAGNSGKEIAVQQLD
                     LLPEDFGSIDQPIADFSIKSDTAFTRLWGTSDKPGLTEITSKNAHSAKSVYLGDTVNN
                     WIYSASGDIARSPDMATSRFIIVSAKGMDINANGNLRDTSVIFHHQDASQHSSITTGR
                     DMLFTEARDTSNKLKDDNGNGILVLGSGSLRVQAGGSINLNTSEGIQSLGQGEVETSR
                     TLTQYNPYLADKAADLFISAGINKTPNYGAVITQYLAKPRMQGKTFVDLIGEATPEQF
                     ISQLNLQLSQPVKTLDDARSQFARLPLWRQQQVALGATRFTNTAAEPNYSRELVAYVT
                     SPSFNKLDLQNALNTELGSKFEGSQAVQNALAALPIEQQHNIALKALGDGGSVLARNF
                     LDGLVLSEIRQGGETAIRQGLKKVDPEGYERGYAALATLYPGIAKDNNPWAGSLAMDN
                     TKIRTSAEADVNILLPGGNFEVGLETPTLAQDTIATKGIGLVVGSYGDINVAAAGSIN
                     VNKSRIFNLGGGDVMLWSSYGDVDAGKGAKTALSVPPPKVQIDPNTGASRLVFPPAVA
                     GSGIQATNPAPSNDPRAAISDGADVLLFAPAGVVDAGDAGISSTGNILVGALEFVGRD
                     NVAGNVTISVASDTSVALPAGAGSVGNDAAQSAEKAADNATGSQKEEKRLAYLTIELL
                     GTGNDDDEDEEEKRKKRAN"
BASE COUNT          864 a          964 c         1002 g          982 t
ORIGIN      
        1 gttcaatggg gcacccagtg gtatattttt taccacaacc gcgccattgc caatgaacgc
       61 ggcaaaagtg attaccagcg ctctgtaaac gtggatttat taaatttcac cgctgatggc
      121 ggcatgaccg taaatgctgg ccgccagggc gtgccgcaac ttaaaaactt taacccctat
      181 aatttagtgg aagcggaaac cttagaaaaa gaatccggcg gtattgaggt ggagcgcgtg
      241 ggtgatgcgt tggttgtagc ctttaccgat aacagctgga ttaaagtagc caaagtggat
      301 tttgaaaccg gcgctgccgg tgtggaatta cgcttagcgg ccgctgccga tagcaaaatt
      361 gaggtgattt tagatgacct taaaaatgcc cccgctgcag ttatccccgt aaaagccact
      421 ggcgatttaa ctaaatttgc tactcaagcg gtgaaattaa gcggccttaa gggtgtgcac
      481 gatgtgtatt accgggtgga gggacgggtt aatgtggata cctataagtt tgtgcgcgaa
      541 gccggtgagg gtgcgctgaa gaaaaaataa ttactctgcg tgagcgttgc gaatttaatt
      601 attgaattaa aggccagctt aatgctggcc tttttatttg gggataattt gtagttggat
      661 tttgcgaggt taattgcaga attagtgaaa gccccacggg cagactttgt ggttacccaa
      721 tacatgcctc ggcgataaat cagaatcaac ggtggatcgg gcacccgcca aggggcgtta
      781 agggttacgc ttgcggacaa ttcactcctc cagcagggcc ccaaattccc cctcctttga
      841 aaggggggcg caatgtcatt aggttaaggt ttattggcgc ttgcacgccg caaaactccc
      901 ctcctttgga ggggtggcag gcctgcctgc cggggtggtt gcggtggcgg gccctgtgtt
      961 tttccagcag cgatgcaatt tttcccagtt aaaggctgta agcggtgaac aaaaagcttc
     1021 gccgggggct accaccccgc ccctttgggg cacccctcca aaggagggga attttaggcc
     1081 ctgctcatgc aggcgccgtc gccactccgg caggcacccc tccaaaggag ggaaattggg
     1141 gcggcccctg ctgggaaata ccgagtggtc agtaggtgct cctgttaatt agcgcgtttt
     1201 ttgcgttttt cctcttcatc ttcgtcgtca tcattacctg tgcctaataa ttcaattgtc
     1261 aggtaagcga gccttttttc ttctttttgg ctgccagtgg cgttatcggc ggctttttca
     1321 gccgattggg cggcgtcgtt gcccacggag cctgcgcctg ctggcagggc tacgctggtg
     1381 tcggaggcga ctgagatggt cacattaccg gccacgttat cccggccaac aaattccaag
     1441 gcgcccacca agatattgcc ggtggagcta atgcccgcat cgcccgcatc caccaccccc
     1501 gccggggcaa ataacaacac atcggcaccg tcgctgatgg cagcgcgtgg gtcgttgctg
     1561 ggggcggggt tggtggcttg gataccgcta ccggccaccg caggcgggaa gactaggcgg
     1621 ctggcgccag tgttagggtc gatttgcact ttggggggcg gcacactcaa agcagttttg
     1681 gcacccttac ctgcgtctac gtcgccgtag gatgaccaga gcatcacgtc gccgccgcct
     1741 aggttaaaga tgcgggattt gttgacgttg atggagcctg cggcggcgac gttgatatcg
     1801 ccgtagctgc ccacaacaag gccaatgcct ttggtggcaa tcgtatcctg ggcgagggtc
     1861 ggcgtttcta aaccaacttc aaaattgccg ccgggcagta ggatatttac atccgcctca
     1921 gcgctggtgc ggattttggt gttatccatg gcaaggctgc ccgcccaggg attgttgtct
     1981 ttggcgatgc cggggtagag ggtggcaagg gccgcgtatc cgcgttcata gccctccggg
     2041 tcgacttttt taaggccctg gcgaatggct gtctcaccgc cctggcggat ttcacttagc
     2101 accaagccat cgaggaagtt gcgggccagt acactgccgc catcgcctag ggcttttagg
     2161 gcaatgttgt gttgctgctc aatgggtagc gccgccagcg cgttttgcac cgcttggctg
     2221 ccttcaaatt tgctaccgag ctcagtattc aatgcatttt gcaaatccaa tttattgaag
     2281 cttgggctgg taacataagc gaccaattcg cggctgtaat taggctcagc ggcagtgtta
     2341 gtaaagcgag ttgcgcccag agcgacttgt tgttgccgcc acaggggcaa gcgagcaaat
     2401 tggctgcggg cgtcatccag ggtttttacc ggctggctta actgtaaatt taattggcta
     2461 ataaactgct caggcgttgc ctcaccgatt aaatccacga aagttttgcc ctgcattctt
     2521 ggtttcgcca aatattgcgt aattacagcg ccataatttg gagttttatt tattccagcg
     2581 gaaataaata aatcggcagc tttatcggca aggtaagggt tgtattgcgt tagcgttcta
     2641 ctcgtttcaa cttcaccctg cccaagagat tggatacctt cagaggtatt cagattgatt
     2701 gagccacccg cctgaacacg caggctaccg gacccaagca caagtattcc atttccattg
     2761 tcatctttaa gtttattgct tgtatcccgc gcctcagtaa aaagcatatc ccggcctgtg
     2821 gtgatactcg aatgctggct cgcatcttgg tgatgaaaaa tcacgctggt gtcgcgcagg
     2881 tttccattag cgttaatatc catgccttta gcactcacaa taataaatct ggatgtggcc
     2941 atgtcgggtg atcgagcgat atcaccgctt gcagagtaaa tccagttatt aaccgtatca
     3001 cctagataga cactcttagc ggagtgtgcg tttttgctag tgatttcagt taaaccaggc
     3061 ttgtctgaag tcccccacaa acgtgtgaag gcggtatcgc tttttataga gaaatccgct
     3121 attggctgat caatcgaacc gaaatcctca ggtaataaat ccaattgttg gactgctatt
     3181 tctttacccg aattccctgc cagctcgcta ttggcaacta tatttccctg cgcagttaga
     3241 ttaagtcgcc catctttttc cggggccaaa ataattgatc catttaggtt gatgtctccc
     3301 tgtacactcg ccacatctaa cttgcttggc aaaatattca gaccaggtgt tcgcgcagta
     3361 gtgggggatt tgctttcatt taaaagtggt tgttttaagt ccatattcat caacaaccct
     3421 acatcgcctg caatactggt aaacgcaaca gcactttgtt tgccgaacgt cgaataataa
     3481 ttatcgactg aatggtcaga ccctaaaaca ctattagcaa ttgcgtcagc cgcaattgga
     3541 ttgctgctga actgccgata actcaaagtc aattgagtgg ggttaaacac cgcctcaacc
     3601 gccaaattcc ccgtggcggt taagggcacc ggcgcatcgc ccagcccaat aaccgccgct
     3661 aactcgcctt cggttttacc catattgcca aggcttgcca cctcaaagcg gttattagct
     3721 gaataccagc ggccagagtt aatatccccc gccgcctgaa tggaaacggc accacttagg
     3781 ctctgctgca cctggttatt aagcccctcg cc
//
LOCUS       DAOQGS010000406         3802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13454_length_3802_cov_4.072591, whole genome shotgun sequence.
ACCESSION   DAOQGS010000406 DAOQGS010000000
VERSION     DAOQGS010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13454_length_3802_cov_4.072591"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1239)
                     /locus_tag="PKZ52_13680"
     CDS             complement(100..1239)
                     /locus_tag="PKZ52_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNG60869.1"
                     /translation="MLKCLFWLAAFTAIFSYFIYPVILRISRLRTNHRLNLNPNAELP
                     KLSLIVTAYNEAKRIEQKITNTLAIQYDPVRLELIVASDCSDDGTDDLVRRYADQGVK
                     LVRASERLGKENAQRAAIAAACGDILVFSDVATEIPADALEKLATYFSDPSVGAVSSE
                     DRFISQDGSIAGEGAYVKYEMWLRKLESEQAGLVGLSGSFFAARASICQEWDIHSPSD
                     FNTALNAAKAGLRAISAPDVLGYYQDLKDPSKEYARKIRTVIRGMTGLWRHKEVLNIK
                     TMGFFAFQVFSHKLMRWLTPWFLLATFLLSALCATAGAFYSLVFIAQLAFYGLALAAH
                     ILPDLRAIGPVKIIYFFVNVNIALLEAGIKFLAGQRMTTWKPSAR"
     gene            1547..2650
                     /locus_tag="PKZ52_13685"
     CDS             1547..2650
                     /locus_tag="PKZ52_13685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNG60870.1"
                     /translation="MVKLERPLRVLHIVSGDLWAGAEAQVFTLLSQLQLMAAPKVVLL
                     NPGELADRLQQLNIPVVILPEANMSSLQIFAHLLKIIYTFKPHVVHTHRQKENILGGA
                     AFFINRLLGRAAVSVRTVHGAPEFIPRGLGRVQEYLNTWVGRHIQAQLIAVSEDLQRK
                     LLATFKPNKLTLVRNGVDEQLLRALLPADTSAENTVNLGIVGRLEAVKRVDLFLAMAA
                     QLIVLAPEIPWQFHVIGDGSLAARLKATAVSLGIDDRVQFHGQVNPVAPLLARLNVIV
                     MCSDHEGTPMVALEALALGIPLVAHKVGGLAELLAGREQLLVSDHSPQGYANKLLAYW
                     RAPGEPVQLPHEYTARYNAESMLALYNRLLGAV"
     gene            2690..>3802
                     /locus_tag="PKZ52_13690"
     CDS             2690..>3802
                     /locus_tag="PKZ52_13690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNG60871.1"
                     /translation="MNNNRTDTKPSLLLVANWDSNVGYAWWLMECYWARIAAQYSDRY
                     RIILAFPTISSIPEHLQTEQIECVEFAFAQGWGIGILKRAQFIWRNRIKLIYLTDHPS
                     YSPLYLLYRCLGVKWIITHDHSPGQRYTPTGIKQFVKSLFNRFSWISASACIGATEYV
                     KERLRRVNCIPSQKCFAAPNGIPLRSLQGDKQVLRRRLGLAEANQIIVTVARANKYKG
                     IDFALDVFALIAQTNPELNFTYLMLGDGPHLAEFKQRAAQLGLAERVVFLGRVESVDD
                     YLMACDIALHPSLGEVGYCLAILEYMLAKLPVVVPDNPSVCGATSAETGVIYPEFNVA
                     AAAQALVELMQQPLVREQKGNASRLAVEEHFNLEKSQ"
BASE COUNT          907 a          872 c         1028 g          995 t
ORIGIN      
        1 agtctaacca cctggcctgg tagccttcga tattcaagcg ctgagacgaa ggttgatggg
       61 tgataatggg ctgcccgacc ggtggcaagt ctccccggat tatcgcgcgg agggtttcca
      121 tgttgtcatt ctctgtccag ctaaaaattt aatgcccgct tcaagcaggg caatatttac
      181 gttgacaaaa aaataaataa ttttcaccgg cccgattgca cgcaggtcgg gcaatatgtg
      241 cgcagctaag gcgaggccgt aaaatgcaag ctgggcgata aatacaaggc tataaaatgc
      301 cccagccgtt gcacataggg cgctgagtaa aaacgtagcc aacaaaaacc agggtgtaag
      361 ccagcgcatt aatttgtggc tgaatacctg gaacgcaaaa aaacccatgg tttttatatt
      421 gaggacttct ttgtggcgcc aaagtcctgt catcccgcga atgaccgtgc gaattttgcg
      481 tgcgtattct ttggagggat cttttaagtc ttgataataa cccagtacgt ccggtgcact
      541 gatggctctt agccccgctt tggcggcatt taatgccgta ttaaagtcgc tcggtgagtg
      601 tatgtcccac tcctggcaaa tggatgcgcg cgccgcgaaa aaagaaccgc ttaagccgac
      661 caaacccgcc tgctcggatt cgagtttgcg cagccacatc tcgtatttaa cataagcgcc
      721 ctccccggca atagagccat cttggctgat aaatcgatct tcgctggaaa cagcgccaac
      781 tgacggatct gaaaaataag tggctaattt ttccaatgca tcggcaggga tttccgtggc
      841 cacatcggaa aataccaata tatcgccaca ggcggcggca attgcggcgc gctgcgcatt
      901 ttctttgccg agccgctcgc tggcgcgcac cagcttcacc ccttgatcgg cgtagcggcg
      961 caccaaatca tcggtgccat catcggagca atcagacgct acaatcaatt ccaagcgtac
     1021 aggatcgtat tgaatggcta atgtattggt tatcttctgt tcgatgcgct tggcttcgtt
     1081 gtaggcggtg acgatcaggc tgagcttggg cagttctgcg ttgggattca gattaagccg
     1141 gtggttggtg cgcaggcgac tgatacgcag aataacgggg taaatgaaat agctgaaaat
     1201 ggctgtgaag gctgccagcc agaacaagca tttaagcatg aggactcctc tttaactagg
     1261 cgagtagggg cgatctaggt agaatcgccg cctttgtgag cacagatatc agccgccaaa
     1321 ggcaggccca tgagtataca tgatcctcaa aatctcggtt aaacctgctg tgctggtcgc
     1381 acgctttgca tgtgagcaaa tatttaggcc gttgtgctgc gctaatgttt tatcaagtgt
     1441 ggacagaaac gcgtcagggg tattgtggtg tggctgtgga attgtgcggt tgcaggccct
     1501 ttagggtgct aaggtgttaa cttaatacag agcaggtaag aactatatgg tcaagctgga
     1561 gcgcccgctg cgagtcctgc atattgtttc gggcgacctg tgggcggggg ctgaggcgca
     1621 ggtatttacg ctcttaagcc aattgcagct gatggcggcg ccgaaagtgg tgctgctgaa
     1681 ccccggtgag cttgccgatc gtttgcagca attgaatatt ccggtggtta tattgccgga
     1741 agcgaatatg tcttcgctgc agatatttgc ccatctgtta aaaattattt acacctttaa
     1801 acctcatgtt gtgcacacac ataggcaaaa agaaaatatt ttaggcggcg ctgctttttt
     1861 tattaatcgt ttattggggc gcgcggctgt gtctgttaga acggtgcacg gcgcgccaga
     1921 atttataccg cgcggcttgg ggcgcgtgca ggaatatctc aatacctggg tcggccgtca
     1981 tatccaggcg caattgattg cggtttccga ggatttacaa agaaaattac tggccacatt
     2041 caaacccaat aaactcacct tggtgcgcaa cggcgttgat gagcagttac tgcgagcgct
     2101 gttgcccgct gatacaagtg cagaaaacac tgttaacctg ggtattgttg ggcgcctaga
     2161 ggcggttaaa cgggtggatt tatttctggc catggccgcg caattaatag tcttggcgcc
     2221 tgagataccc tggcaatttc atgtgatcgg tgacggcagt ttagccgcgc gattaaaagc
     2281 gacggcggta tctttaggta ttgatgatcg cgtgcagttt cacggccagg taaatccggt
     2341 ggcgccgcta ttggcgcgtt taaatgtaat tgtaatgtgc tctgatcacg agggcacacc
     2401 tatggttgcc ttagaggcgc tggccttggg aatacccttg gttgcgcata aggttggcgg
     2461 cctggcggaa ttattggcgg gccgcgagca attgttggtg agcgatcatt cgccacaggg
     2521 gtacgccaat aaactcctcg cctattggcg tgcaccgggt gagccagtgc aattgccaca
     2581 cgaatacacc gctcgataca acgcggagtc tatgttggcg ttgtacaatc gcctattggg
     2641 cgcagtttaa attaactttg tctgtttttg ttggagaatc ccgtttgtca tgaataacaa
     2701 ccgcacggac actaaaccat cgttattgct ggtggccaat tgggattcta atgtcggtta
     2761 tgcctggtgg ttaatggaat gttactgggc gcgcatcgct gcgcagtaca gcgatcgcta
     2821 tcgaattatt ttggcttttc caacgatttc ttccattcca gagcacttac aaacggagca
     2881 aatagaatgt gttgagtttg cctttgcgca aggctggggt attggcattc tcaaacgggc
     2941 gcaatttatt tggcgtaacc gaattaaatt aatctaccta actgaccacc ctagttattc
     3001 gcctttatat ttactgtatc gctgtttagg ggtgaaatgg attatcaccc atgatcactc
     3061 gccgggccag aggtatactc cgacgggcat caaacaattc gtaaagtcat tattcaaccg
     3121 tttcagttgg atatctgcca gtgcctgtat tggtgctacc gaatatgtca aagagcgttt
     3181 gcgccgcgtt aattgtattc ccagccaaaa atgttttgcc gcgcccaatg gaatacccct
     3241 gcgcagtttg caaggggata aacaggtttt gcgcaggcgg ttaggtcttg ctgaggcaaa
     3301 ccagataata gtaactgttg ccagagccaa taaatataaa gggattgatt ttgcactgga
     3361 tgtatttgca ctgattgcac aaactaatcc cgagcttaat tttacctact taatgctggg
     3421 tgacggcccc catttggcgg aattcaaaca gcgggcggcg cagctggggc ttgctgagcg
     3481 ggttgtattt ctcgggcgag tggagtccgt ggacgattat ttaatggcct gtgatatagc
     3541 cttgcacccc tctttaggtg aggtgggtta ctgcttggct atattggaat acatgctggc
     3601 aaaattgccg gtggtagtgc ctgataatcc ctctgtctgc ggcgctacca gcgccgaaac
     3661 cggtgtcatc tatcctgaat ttaatgttgc tgctgcagcg caggcgctgg ttgagcttat
     3721 gcagcagcct ctggtgcgcg agcaaaaagg caacgcgagc cgattggcgg ttgaagagca
     3781 ttttaatctt gaaaaatcac aa
//
LOCUS       DAOQGS010000407         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13572_length_3781_cov_3.297370, whole genome shotgun sequence.
ACCESSION   DAOQGS010000407 DAOQGS010000000
VERSION     DAOQGS010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13572_length_3781_cov_3.297370"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKZ52_13695"
     CDS             <1..802
                     /locus_tag="PKZ52_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG60872.1"
                     /translation="MIDRYPRPLGFLDSDLTETFVQAFEEMGGTWVGNSKVHKVYWNG
                     FNAVITELEDGRTISSDKLLCAAGRVSNVADLKIENAGLKLNDKGLIEVDASLATCVP
                     HIYAAGDIIGPPSLASASMEQGRRASSNALGCPVGAMGDLIPSGIYSIPELSGVGLTE
                     EQARKAYGDVIIGKAKFDEIARGQISGTQNGMLKIICAPDGEKILGVMIVCEGATELI
                     HIGQMALITQSHVDIFVENIFNFPTLAEAYRVAALEIIGKRSARQHVF"
     gene            complement(849..1097)
                     /locus_tag="PKZ52_13700"
     CDS             complement(849..1097)
                     /locus_tag="PKZ52_13700"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HNG60873.1"
                     /translation="MSAIAYSLYAWDKRAAQASRWRISERNLHLVALLGGWPGAIFAQ
                     QRLRHKSQKLSFRVIFYSTVLINVALLIAYGWRQFWAF"
     gene            complement(1195..2625)
                     /locus_tag="PKZ52_13705"
     CDS             complement(1195..2625)
                     /locus_tag="PKZ52_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNG60874.1"
                     /translation="MVNFSHPLLQRLVRKKPLDLEQPNELKRCLSALDLTLLGIGAII
                     GTGIFVLTGIAAATTSGPAVVVSFIVAGLACAFAAFAYAELSALVGGSGSAYGYSFAA
                     FGELVAWIIGWDLILEYGVAVAAVANGWAGYFNNALHVIGIDLPVYLTRGPWAGGELG
                     AGWVNLPAFIIVLLLTIMLAIGVKQSAKLNTVMVFVKLLTILVFLSVGIFHVDSAHWS
                     PFAPFGWFSHDELGKPIGILAGASVVFFAYVGFDAVSTATEEANKPQRDIPLGILASL
                     IFCTLIYIVVSGVLTGILPYTQLNVPSPVAFALESIGIKWASALVAAGVIAGLTTVML
                     VLFYALTRIITAMGRDGLLPEVFAEVNPKTQTPVKNTVLCGLVMAVMAGFIPLGTLAE
                     LVNVGTLFAFVLVCLGVIVLRYTQPDAPRPFKIPAGILVCVLGALSCSALIAFLPHHT
                     YERFVYWLAAGLVIYFSYGFYHAKRA"
     gene            complement(2659..2898)
                     /locus_tag="PKZ52_13710"
     CDS             complement(2659..2898)
                     /locus_tag="PKZ52_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HNG60875.1"
                     /translation="MNKTVMISQQVNRFVRVWRLCQQTANLMVGQGDYQQYLTHMKSH
                     HPELTPMSEKEFFRYHQNSRYPQARGKANINRCPC"
     gene            complement(2895..>3781)
                     /locus_tag="PKZ52_13715"
     CDS             complement(2895..>3781)
                     /locus_tag="PKZ52_13715"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNG60876.1"
                     /translation="IYFALNSPAAVIGKTVEEAAGVISQWGFVITPEMLTQMAQDVGE
                     ASILSRTGGAPSFALGMANILAGFLGGKALMAFWYHFAILFEALFILTTLDAGTRVCR
                     FLIQDLLGSAVPSLANTQSWGANILATFLAVAGWGYFLYVGVTDPFGGINSLWALFGI
                     ANQMLAGIALILVSVLLLRSGKGQYVWISALPAAWILLITLYASGLKLLSADPKIGFL
                     AHAQKYAQGLADGKILPPTQSISQMQQVVFNDYVDAVMTLIFICVVISILGFALRCLW
                     QHRRNGAYITTESVEVTP"
BASE COUNT         1200 a          967 c          804 g          810 t
ORIGIN      
        1 catgattgat cgctatccgc gcccactggg atttttggat agcgatttaa ccgaaacatt
       61 tgtgcaggcc tttgaagaaa tgggcggcac ctgggttggc aatagtaaag tacataaggt
      121 ttattggaat ggttttaatg cagttattac tgaactggaa gatggccgca ccatctcttc
      181 agataaacta ctctgtgccg cagggcgtgt ttcaaatgtt gcggatttaa aaattgaaaa
      241 tgcaggttta aaacttaacg ataaggggtt gatagaagtc gatgcctcct tagctacctg
      301 tgttccgcat atttatgcgg caggcgatat tattggcccc ccctccttag cctcagcctc
      361 catggaacaa gggcgcagag ccagtagcaa cgctctaggc tgtccagtcg gcgccatggg
      421 ggatttaatt ccttccggta tttattccat accagaactt tccggtgtgg gcttaacgga
      481 agaacaagcg cgtaaagcct atggtgacgt gattataggt aaagccaagt ttgatgaaat
      541 agcccgcggg caaatatccg gcacccaaaa cggtatgtta aaaattattt gcgcgccgga
      601 cggggaaaaa attctcggtg ttatgattgt atgtgaaggg gcaacagaac tgattcacat
      661 aggccagatg gcacttatta cgcaatcaca cgtggatatt tttgtagaaa atatttttaa
      721 ttttcccacc ctcgctgaag cctaccgagt agctgcacta gaaattatcg gcaaacgctc
      781 cgcccgccag catgtttttt aaaattcttt gcctcaaaaa atgaagcgca cattcaggat
      841 gcaactgctt aaaaagccca aaattgccgc cagccatatg caatcagcaa agcgacatta
      901 atgagtaccg ttgagtaaaa aataaccctg aaggataact tttgggattt atgccgcaag
      961 cgctgctgcg caaaaatcgc acctggccag ccacctaata atgcaaccag atgtaggttg
     1021 cgctcgctaa ttcgccagcg gctagcctgt gctgcgcgct tatcccaggc atataagctg
     1081 taggcaatcg cgctcatggc acaatagccc atgagcacat acgaactcat ataaccgcat
     1141 gccaccaaaa cacccaacag cgcaacaaac aaaatcggaa tgaacacgcc taaatcaagc
     1201 gcgtttagcg tggtaaaagc cataactaaa ataaatcacc aagcccgccg caagccaata
     1261 cacaaaacgc tcataggtat ggtgtggcaa aaaggcaatt agtgctgaac aggacagagc
     1321 gcccaacaca cagactaaaa tacctgccgg gattttaaat ggacgcggcg catcaggctg
     1381 ggtgtagcgc aacacgatca cacctaaaca aaccaacaca aaagcaaata gggtccccac
     1441 gttcaccaat tccgctaaag tgcccaacgg aataaaacct gccataaccg ccataacaag
     1501 gccacataaa actgtatttt taactggcgt ttgagttttc ggattcactt cagcaaaaac
     1561 ttcaggcaat aaaccatctc gccccatggc ggtgataatt cgcgtgaggg cataaaacaa
     1621 caccaacatt actgtagtta agcctgcgat aacccccgca gctacaaggg cggaggccca
     1681 cttaatacca atgctttcaa gggcaaatgc aactggtgag ggtacattta attgtgtata
     1741 gggcaatatg ccggttaaca cgcccgatac cacaatataa attaatgtac aaaaaataag
     1801 agaagccaga ataccaagag gaatatcgcg ctggggctta ttggcctctt cggtagcggt
     1861 ggatacagca tcaaaaccca cataagcaaa aaacaccact gaagcgccag ccaaaatacc
     1921 aatcggctta cctaactcgt catgggaaaa ccaaccaaag ggcgcaaatg gactccaatg
     1981 ggcgctgtcc acatgaaaaa tgccgacact caaaaacacg aggatagtaa gcagttttac
     2041 aaacaccata accgtattga gtttagcgct ctgtttaaca cctatagcca gcataatagt
     2101 cagcagcagc acaatgataa acgcgggcaa atttacccag ccagcaccca gctcaccccc
     2161 cgcccagggc cctcgggtta aatacaccgg caaatcaatg ccaatcacat gcagggcatt
     2221 attaaaataa cccgcccacc cattggccac ggcagctacc gcaacgccat attctaaaat
     2281 taaatcccaa ccgataatcc aggccacaag ctcgccaaaa gcagcaaacg aataaccata
     2341 ggcgctgccg gaaccgccca ccaatgcaga gagttctgca taggcaaagg cggcaaaggc
     2401 gcaggcgagc ccagccacaa taaacgacac cacaactgca gggcctgagg tagtagccgc
     2461 cgctatgccc gttaacacaa atatgccggt gccaataatg gcacctatac ctaatagagt
     2521 taaatcaagc gcgcttaaac agcgctttaa ttcattgggc tgctctaaat caagcggctt
     2581 tttgcgcacc aagcgttgca acagcggatg agagaaatta accatgatga catctcaaga
     2641 aaataaaata atggagccct aacaggggca gcgattaata ttggccttcc cgcgagcctg
     2701 ggggtaacgt gaattctgat ggtaacgaaa gaattctttt tcagacatag gggttaactc
     2761 tgggtgatgg ctcttcatat gggtgagata ctgttgataa tccccctgac ccaccatcaa
     2821 attggccgtt tgctgacaca accgccacac gcgcacaaaa cgattcacct gctgagaaat
     2881 cattaccgtt ttattcatgg tgtcacctcc acagactctg tggttatata ggccccgttg
     2941 cgacgatgct gccaaaggca acgtaatgca aagcccaata tgcttattac aacgcagata
     3001 aatataaggg tcataacggc gtcaacataa tcgttaaaga ccacctgctg catttgactt
     3061 atcgattgcg tgggcggcaa tatttttcca tccgccaaac cctgcgcata tttctgggca
     3121 tgggctaaaa agccaatttt aggatctgcg gataaaagct ttaaaccgct ggcatagagt
     3181 gtgattaata aaatccacgc agcaggtaat gcactgatcc aaacgtattg ccctttaccg
     3241 ctgcgcagca acaacacact aaccagaatt aaggcgatcc ccgccaacat ttgattggca
     3301 ataccaaaca aagcccacaa actattaata ccgccaaagg gatcggtaac ccccacatac
     3361 aaaaaatacc cccaaccggc aacggctaaa aaagttgcca gaatatttgc cccccaagac
     3421 tgggtattgg caagcgatgg cactgcacta ccgagtaaat cttgaattaa aaagcggcag
     3481 acacgggtac cggcatctaa agtcgttaaa ataaataagg cttcaaataa aatggcaaag
     3541 tgataccaaa aggccatcaa tgctttaccg cctaaaaatc ccgctaaaat attcgccata
     3601 cccaaagcaa aactcggtgc gccaccggtg cgcgataaaa tcgaggcttc acctacatcc
     3661 tgggccattt gggttaacat ctctggagta ataacaaacc cccactgact gatcacaccc
     3721 gcagcttctt caaccgtttt gccaatcacc gcagcagggc tgtttaacgc aaaataaata
     3781 c
//
LOCUS       DAOQGS010000408         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13659_length_3763_cov_5.368123, whole genome shotgun sequence.
ACCESSION   DAOQGS010000408 DAOQGS010000000
VERSION     DAOQGS010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13659_length_3763_cov_5.368123"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKZ52_13720"
     CDS             complement(<1..1534)
                     /locus_tag="PKZ52_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60877.1"
                     /translation="MNDTIKLALNLNRKEYTGEDKLVVYAFSAGGKYLAQAPANTDAL
                     DLALDGRYDGRDITLAIAPTPEGTNPTTAPSLATLKKLGAHLTHARALRDIKGIDIVI
                     PPIIFPKICFCTVRGKLVKRFTLPDGTQVQRPVCNARVHICEIDSIPLIIDRIPDLDI
                     NRLRESLLDKLRVIPRPFPPGPDPDPIIRLDTPLALQARTLAKPLAATQPLAPDLSQA
                     VIALSANQTPSVLRRTLIDLRALISIHLCEFKFLWKYFKKTCHTTLYVDGDGQFNGVI
                     THRCTDQPDIYIWVEQYYEGAWHTVYNPSIPCGTYWDYKCGTEITLNLPKAHACDRPT
                     IDLPDGVVNFVLPYKIGETTIWGKPGTPAPFGWVRSDGLVDYASSPLGNLYNAPFGGI
                     LNFYHDDSYFIPSTAIKYYRYSFRRFSATPNSGPTDPSWTPITTALARGYRMEYSDRL
                     PTYESYPVGPFSVGSHSNLFKFKPQTPPARSTDPATVVVREWLSGNLNDVAASWNTLI
                     PAP"
     gene            2057..2608
                     /locus_tag="PKZ52_13725"
     CDS             2057..2608
                     /locus_tag="PKZ52_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNG60878.1"
                     /translation="MQDDAALIRAAQNGERAAFGELVGRYYNTLYRFAYRWCGNREEA
                     EDITQQACIKLARSLGQFRFEAAFSSWLYRLVINCAKDWRSSQHRHRGEALTPNTEEP
                     VAMPQVGDAEIYLQQVLRQLEHMGEHYKETALLVFAEGMSHAEAASVLEVKESTVSWR
                     LHEIRKQLQLLQRTQPGSWEVEP"
     gene            2605..3414
                     /locus_tag="PKZ52_13730"
     CDS             2605..3414
                     /locus_tag="PKZ52_13730"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG60879.1"
                     /translation="MNDDQLKQLFAAQNIPAPSPAAKAAALQAAMAVFAEQNTLASPI
                     SAQEQNIINRADEIANNIQAHKTRYSINSLAARFYGLFAVAGLHAAVIIALMVGLAPK
                     NEMVLVDNVKVQTVEELKEEIALPPPPPPDYVPPPPDFIPPPSFNVAAEAPAPANAIT
                     SVAAKAEAAPAAKAPPTPARPPKKGLAPPSYPSESKKLGEEGAVALALYLNEEGKVQE
                     ARVETSSGFPRLDEAAVKHAVKAWKFEPCTEAGKPVACWYKIKFRFQLKDA"
BASE COUNT          954 a          893 c         1031 g          885 t
ORIGIN      
        1 ccggtgcggg tatcagtgta ttccagctgg cggcaacgtc gtttaaatta ccgcttaacc
       61 attcgcgcac taccactgtt gctgggtctg tgctgcgtgc gggcggtgtc tgcggtttga
      121 atttaaataa attgctgtgg ctgcccacac taaacggccc tactggataa ctctcatagg
      181 tgggcaagcg gtcggaatat tccatgcgat aaccgcgcgc taaagcggtg gtgatgggtg
      241 tccagctggg atcggtgggg ccgctattgg gggtggcgga aaatcggcgg aaggagtagc
      301 gataatattt aatggcggtg gacggaataa aataagaatc gtcgtgataa aaattgagta
      361 tgccgccaaa gggtgcattg tataaattac ccagtggact gctggcataa tcaactaagc
      421 catcactgcg cacccagcca aaaggcgcgg gtgtgccggg tttgccccag atggtggttt
      481 cgccaatttt atagggcaaa acaaaattca ctacaccgtc gggtaggtcg attgtgggcc
      541 ggtcacaggc gtgggctttg ggtagattta atgtgatttc ggtaccgcat ttataatccc
      601 aataggtgcc gcaggggatg ctggggttat ataccgtatg ccaggcgcct tcataatatt
      661 gttcaaccca aatataaata tctggctgat ctgtgcagcg gtgggtaatt acgccgttaa
      721 attggccatc accgtccaca tataaagtgg tatggcaggt ttttttgaaa tacttccaga
      781 gaaatttaaa ttcacacaga tgaatagaaa taagtgcgcg caaatcaatt aatgtgcggc
      841 gcagtacgct gggggtttgg ttggcgctaa gcgctatgac cgcctgggag agatccggcg
      901 ctaggggttg agttgccgct agcggtttgg ccagggtgcg ggcttgtaaa gccaagggcg
      961 tatccaggcg gataattggg tcgggatcgg ggccgggcgg aaatggcctt ggaatcaccc
     1021 gcaatttatc cagcaggctc tcccgcagcc ggttaatatc caagtcggga atgcggtcga
     1081 taattaatgg aatagagtct atttcacaaa tatgtacgcg ggcgttacac acagggcgtt
     1141 gcacttgggt gccatcgggt agcgtaaaac gcttcacgag tttgccgcgc accgtgcaaa
     1201 aacatatttt tggaaaaatt atcggcggga taacaatatc aatacctttt atatcccgca
     1261 gggcgcgcgc atgggtgaga tgggcgccga gttttttcaa tgttgcaagg ctcggtgcag
     1321 tggtagggtt tgtgccttcc ggggtaggcg ctatggcgag tgtgatgtcg cgcccatcgt
     1381 agcgcccatc cagtgctaaa tcgagcgcat ctgtgttggc tggtgcctgc gccaagtatt
     1441 taccgcctgc tgaaaatgca taaaccacta gcttgtcttc gccagtatat tctttgcggt
     1501 ttaaattgag tgccagttta atcgtatcgt tcataacgga ctccaaaaat ataggttatt
     1561 gtttggcaca agcggcgttg ctgtgcatat ctgttatgcc gacggtaaaa agtggcaggg
     1621 cagatcctaa aaccctattg gaatgtgcat gtaattgccc ggcactattt aacgggataa
     1681 atgggtaata atgccatttt gactgtggcg gttaattggt gctgggtatt ggtgggagga
     1741 attggcggcg gtggcaagaa tttatacgtg ttccagaagt gataaaagta gtaataatgc
     1801 cagaggtaaa aaatagtaac tgatcagcac cgcctgtttg ccatccccac cattaaaaac
     1861 acagtcttac ccgtgcacca ttactggctc ggattcgcta ccgtcacaca aactgagtgg
     1921 agggcgggtg tgacaaatac tcatgtggtg tatatcctgg cgagcttgca tgcgcccaag
     1981 gattgctccg catgccccgt cttataaaac agtgcgaacc acgagccccc aatgtaccgt
     2041 ccagacgtat ccccccatgc aggatgacgc cgcccttatt cgcgccgccc aaaacggtga
     2101 gcgcgccgcc tttggtgaat tagtcggccg ttattacaac accctttacc gctttgccta
     2161 ccgttggtgc ggcaaccgcg aagaggcgga agatattacc cagcaggcct gtatcaaact
     2221 ggcgcgcagc ctcgggcaat ttcgttttga ggcggcattt agcagctggc tgtatcggtt
     2281 ggtaattaat tgcgcaaaag attggcgcag cagccagcac cgccaccggg gtgaggcgct
     2341 aacgccaaat accgaggaac cggttgcaat gccgcaggtg ggagacgccg aaatatattt
     2401 acagcaggtg ttaaggcaat tagaacacat gggcgagcac tataaagaaa cggcattatt
     2461 agtatttgcc gaaggtatgt cccacgccga agcggccagt gtattggagg taaaagagag
     2521 caccgtatcc tggcgcctgc acgaaattcg caaacagctt caattgctgc aacgtacaca
     2581 accgggcagc tgggaggttg agccatgaat gacgaccaat taaaacaatt atttgccgcg
     2641 caaaatattc ctgccccatc acctgcggcg aaagcggcgg cattacaagc cgccatggcg
     2701 gtttttgccg agcagaatac attagcctcg cccatatccg cgcaggagca gaatattatt
     2761 aaccgcgctg atgaaattgc taacaacata caggcgcaca aaacccgtta ttccataaat
     2821 agtttggccg cgcgattcta cggattattt gccgtagcgg gtttacatgc ggcggtgatt
     2881 atcgccttga tggttggcct tgcaccgaaa aatgaaatgg ttttggtgga caacgtaaag
     2941 gtgcaaacgg ttgaagaatt aaaagaagaa attgcactgc ctccgccgcc accgccagat
     3001 tatgtaccgc caccaccgga ttttattccg ccgccttcat ttaatgtagc tgcggaagcc
     3061 ccggccccag ccaatgccat tacctcagta gcggcaaaag ctgaggctgc gcccgctgcc
     3121 aaagcgccac caacccccgc ccggccaccc aaaaaaggcc tggcgccgcc gagttatccg
     3181 agtgagtcaa aaaaactcgg cgaagagggc gccgtggcct tagcccttta tttaaacgaa
     3241 gagggtaagg tgcaagaggc gcgggtggaa acttccagcg gcttcccccg tttagatgaa
     3301 gccgcagtaa aacatgcggt taaagcctgg aaatttgagc cctgtaccga agcgggtaaa
     3361 ccggttgcct gttggtacaa aattaaattt cgctttcagc ttaaagatgc ttaatgctgg
     3421 gtgtaattgc cacccaggcg ggccgcccgc ttttaaaagc agcggctggc ttggcagggc
     3481 aaccgagcaa aattgaatca tttggctgcg cgcaaaatta tatttaaatt gctccgtaac
     3541 gaattatctc tgcgggctga gtattgaggg cttgcagcac tcaatactca gccgatattt
     3601 tttagctggc gcgatcatca ccttcaatta ccctaaacgg ggacttatta acccggaatt
     3661 agtgccgggt taataaaggc ggcaaggatg ccgcgtcaaa ggtgcaaacc gccgcgagct
     3721 gcactaaaac aagaaaatcc gcgcattttc gtaagtttag tgc
//
LOCUS       DAOQGS010000409         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13672_length_3761_cov_4.446573, whole genome shotgun sequence.
ACCESSION   DAOQGS010000409 DAOQGS010000000
VERSION     DAOQGS010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13672_length_3761_cov_4.446573"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKZ52_13735"
     CDS             <1..261
                     /locus_tag="PKZ52_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60880.1"
                     /translation="PGVVRSGFFANATPLMRAALWLVGDLLLISPTAGAKTILHLATR
                     EQLTASGQYFVHCAPKAPAKFALDDEACERLWQESERFTQLA"
     gene            405..2111
                     /locus_tag="PKZ52_13740"
     CDS             405..2111
                     /locus_tag="PKZ52_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNG60881.1"
                     /translation="MANLRSLFSRSGCCLKSFNTLLRLSGIGALSLSLLVSGCGNDKE
                     AVSSKAKEGYKELDDLYIVDCLLPNQVRRLGNTSYLPPRKAIKTTTSDCRIRGGEYVD
                     YNRADYRSALNVWLPQAQAGDAEAQNYVGEIFEKGLGQTPDFASAVSWYKKSADQGYA
                     RAQINLGYMYEKGQGVPRDMTLALNYYRQASGIKNDTLVLNSDAQKEIAANKQQLADA
                     LQKAQTQANVLQKQVAGLQTKLTKQEALAAKSPQAAQELASVRQELEAMKSLYEKAQG
                     DKSSISSELQSVELAYRKFSDQPLLTNETLANSAPRTFKDINFGRYYALIIGNQDYAF
                     LEPLRSPISDARKLKDVLEAKYGFSTLLIENGSEKQILNTINNFYSQIGENDNLLIFY
                     AGHGQLSTGENSKKERGYWLPVDAKANTLSTWINNAVISDHLDRLKARSILVIADSCY
                     AGFLGSEKSPLLFGVSSSQITEAGIKASLNRRARLVISSGGLAPVLDGTNSNHSVFAN
                     ALIETLEHNNDTLRDSNLFSALSVNVRKRASAADLVQMPEMKPVREAGHAGGTFYFVP
                     KI"
     gene            2155..3585
                     /gene="mgtE"
                     /locus_tag="PKZ52_13745"
     CDS             2155..3585
                     /gene="mgtE"
                     /locus_tag="PKZ52_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNG60882.1"
                     /translation="MAAARSDNLQDTLEPIFALLQKQELVESLAHRQSGSANPLVEQL
                     VHRQHEAELRRKLHNLNAADIAHLLDMLPQKKRLLVWSLVSDQQAAECLLELSENSLH
                     HVFAETPAEQLKNLLGRLEADELAVIADFVPEDVLTAVKAGLESGERDWLETTLNYDE
                     ESVGSLMSRDSLVVYSSWTVDQAIDSIRQLDELPEQTDQVFVVNEQRVLVGALPLTAL
                     LRHRGQEQLKTIMNTQVVRFLPTTEGQEAALAFERYDLICAPVVDERGRIIGRLTVET
                     IMDFVREQNEHMELAKQGLSARADLLDPIWQGAQTRWLWLAINLITAFCATRFIALFA
                     SSIESLVALATLMPIVASIGGNAGQQTAALMVRSLSLDQIHKNNLPLALGKEIAVSLI
                     NGLIWGSLLGAVAAAIYGNLGLGLVMLAAICLNLLIAAVAGIFTPLLINNLGRDPAMG
                     TSVILTFITDSMGFFIFLGLATLWLI"
BASE COUNT          960 a         1011 c          913 g          877 t
ORIGIN      
        1 ccgggtgtgg tgcgcagcgg tttttttgcc aatgccacac cattaatgcg cgccgccctg
       61 tggctagtgg gcgatttatt attaatttca cccacggcgg gcgccaaaac catcctgcat
      121 ttagccaccc gcgaacagct taccgcctcc ggccaatact ttgtgcactg tgcgccaaaa
      181 gcccccgcta agtttgcctt ggatgatgag gcgtgcgaac ggctgtggca ggaaagtgag
      241 cgatttaccc agctcgccta gcatatccat taacacagat gctaaagccg gcagcgccaa
      301 tagggtgcaa aatcctgcta tgctgccgcc caggcgcgca agcgtgcaac caggtcaaat
      361 catccagcgg cccaccgctg ccattcattc tttcgaggtg cttcatggcc aatcttcgtt
      421 ccttgtttag ccgttcaggc tgctgcttaa aatcgtttaa taccctgctg cgcttatccg
      481 gcataggtgc actcagccta agcctactgg tgagcggttg cggcaatgat aaagaagcgg
      541 tcagcagcaa agccaaagaa ggctacaaag aattggacga tttatacata gtggattgtt
      601 tgctgcccaa ccaggtgcgc cgcctgggca atacctccta cttaccgccg cgcaaagcca
      661 ttaaaactac cacctctgac tgccgcattc gcggtggcga atatgtggat tacaaccgcg
      721 ccgattaccg ctccgcctta aatgtatggc tgccccaagc ccaagcaggc gatgctgaag
      781 cgcaaaatta cgtgggggaa atttttgaaa aaggcctcgg ccaaaccccg gattttgcct
      841 ccgccgtcag ctggtataaa aaatccgccg accaaggcta cgcccgcgcg caaattaacc
      901 tgggttatat gtacgaaaaa ggccagggcg taccgcggga tatgaccctg gcgcttaatt
      961 attaccgcca ggcctccggc attaaaaacg ataccctagt gcttaattcc gatgcccaaa
     1021 aagaaatcgc cgctaacaaa caacagctag ccgatgcact gcaaaaagct caaacccaag
     1081 ccaatgtgtt acaaaaacaa gtggccgggc tgcaaactaa attaaccaaa caagaagccc
     1141 tcgccgcaaa aagcccgcag gccgcccagg aacttgccag cgtacgacag gaattagagg
     1201 ccatgaaatc cctctatgaa aaagcccagg gcgataaaag cagcatatcc agcgaattac
     1261 aatcggtgga attagcctac cgcaaattct ccgaccagcc cctattaacc aatgaaaccc
     1321 tcgccaacag cgccccgcgc acgtttaaag atattaattt tggccgctat tacgcgctga
     1381 ttatcggcaa ccaggattac gcctttttag aacccctgcg ctcccctatt tccgatgcgc
     1441 gcaaattaaa agatgtgctg gaagctaaat atggctttag tacactattg attgaaaacg
     1501 gcagcgaaaa acaaatcctc aataccatta ataattttta cagccaaatt ggcgagaatg
     1561 ataatttatt gattttctac gctggccacg gccaattatc caccggcgaa aacagcaaaa
     1621 aagagcgcgg ttattggttg ccggtggacg ccaaggctaa cactttaagc acctggatta
     1681 acaacgcggt tatttccgac cacctggacc gcttaaaagc ccgctctatt ttagtaattg
     1741 ccgattcctg ttatgcaggc tttttaggct cagaaaaaag ccccttatta tttggcgtct
     1801 cctccagcca aattactgaa gctggcatta aagccagcct caatcgccgc gcccgcttgg
     1861 ttatttcctc cggcggccta gcaccggtac tagacggcac caacagcaac cactcggtgt
     1921 ttgccaatgc gttaattgaa acccttgagc acaataatga caccctgcgg gatagtaatt
     1981 tattctccgc cctgtcagtc aatgtgcgca aacgggccag cgcagctgat ttagtgcaaa
     2041 tgccggaaat gaaaccggtg cgggaagctg gccacgcagg gggcaccttc tattttgtac
     2101 ctaaaattta aaactgctgt gccagcagcg ttttaagctg agcaatggcg ccttatggcc
     2161 gcagcccgca gcgataattt acaggatacc ctagagccca tttttgcttt attgcaaaaa
     2221 caggaactgg tggaatcctt ggcccatcgc caatcgggct cggctaatcc cttagtagag
     2281 caattagtcc atcgccaaca cgaagccgaa ttgcgccgca aattacacaa tttaaatgcg
     2341 gcggacatag cccatttact ggatatgctg ccgcaaaaaa aacgcttgct ggtgtggagc
     2401 ttagtgagcg accaacaggc cgccgagtgt ttgttggaat taagtgaaaa cagcctgcac
     2461 catgtatttg ccgaaacgcc cgccgagcag ttaaaaaatc tcttaggccg cttagaagcg
     2521 gatgaattag cggtaattgc cgattttgta ccggaagatg tgctcaccgc tgttaaggcg
     2581 ggactggaaa gcggcgagcg ggactggctg gaaactacct taaattacga tgaagaatcc
     2641 gtcggcagcc ttatgagccg ggattcatta gtggtgtaca gttcctggac ggtggatcag
     2701 gccattgact ccatccgcca actagacgaa ttacccgagc agaccgacca ggtgtttgtg
     2761 gtgaacgagc agcgggtatt ggtgggcgcc ctgccgctga cagccctgtt gcgccaccgg
     2821 gggcaagaac aattaaaaac cattatgaat acacaagtgg tgcgcttttt acccaccact
     2881 gagggccaag aggcggccct cgcgtttgag cgctacgact taatttgtgc ccctgtggtg
     2941 gatgagcgcg gccgtattat tggccgctta acggttgaaa ccatcatgga ctttgtgcgc
     3001 gagcaaaacg aacatatgga attggccaaa cagggtttga gcgcccgcgc cgatttatta
     3061 gaccctatct ggcaaggcgc ccaaacccgc tggttgtggc tggccatcaa tttaattacc
     3121 gccttttgcg ccacccgttt tatcgccctg tttgcctcct ccattgaatc cctagtggcc
     3181 ctcgccacac tcatgccaat tgtcgccagc atcggcggca acgcaggcca acaaaccgcc
     3241 gccttaatgg tgcgcagttt aagcctggat caaattcata aaaataattt gcccctagcc
     3301 ctgggcaaag aaatagcggt aagcctaatt aacggtttaa tctggggcag tttattgggc
     3361 gctgtggcgg cggctattta tggcaatcta ggcctgggtt tggtgatgct ggcagcgatt
     3421 tgtttaaacc tattgattgc agctgtagcg ggcattttca cgccgctgct gattaataac
     3481 ctgggccgcg acccggccat gggaaccagt gttattctca cgtttattac cgatagcatg
     3541 ggttttttta ttttcctcgg cttggccact ctttggctaa tttagtacaa caggtttttt
     3601 gcaaaatcca gggtgattat caacactgct ttttctctcc cggcactgca cgcgctttaa
     3661 tcttgcccgc agtaattaat aaggggtatt tcagcgacgc cctattaagt gctttggcgc
     3721 tgggagccct caatggtccg cccgcagtgg tgaatacgag t
//
LOCUS       DAOQGS010000410         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13739_length_3749_cov_5.486194, whole genome shotgun sequence.
ACCESSION   DAOQGS010000410 DAOQGS010000000
VERSION     DAOQGS010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13739_length_3749_cov_5.486194"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..846
                     /gene="ribE"
                     /locus_tag="PKZ52_13750"
     CDS             385..846
                     /gene="ribE"
                     /locus_tag="PKZ52_13750"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNG60883.1"
                     /translation="MNKIEGDFKQSQGRYALLVSRWNSFVVEHLKDGAIDTLQRHGIP
                     DANITLVYAPGAFEFPLAAQKLARSGKFDAIIALGAVIRGSTPHFDYVAGECTKGLAQ
                     VSLDAGIPVSFGVLTVDSIEQAIERSGTKAGNKGAEAAATALEMVSLLSKI"
     gene            914..1357
                     /gene="nusB"
                     /locus_tag="PKZ52_13755"
     CDS             914..1357
                     /gene="nusB"
                     /locus_tag="PKZ52_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNG60884.1"
                     /translation="MPSTPAARSKARHYAMQALYQWQMTKNPINNILAEFHADNDMNV
                     VDVPYFHELVHGVPAEITTLETDFLPFVNDMSLAQIDPITLAILRMACFELRHRIDVP
                     YRVVINESLNLAKKFGATDSHKFINGVLDKVAGRLRSVEVNAGKN"
     gene            1361..2293
                     /gene="thiL"
                     /locus_tag="PKZ52_13760"
     CDS             1361..2293
                     /gene="thiL"
                     /locus_tag="PKZ52_13760"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNG60885.1"
                     /translation="MNEFTLIQQYFAALTPPCASVPQAIGDDCALLAPPEGKLLAVSI
                     DTQVEGVHFPVGAAAEQIGRRVACCALSDLAAMGAQPLWATLALTLPKADATWLAAFS
                     RGLGAVLNTWQVRLVGGDTTCGPLTISLQVHGSVASEHALRRSGARAGDCIYVTGPLG
                     DGAAGLALLQGQLSLPAEAAAYLTERFYAPTPQIAAGLALAGRATSAIDISDGLLADL
                     NHILTASQVGARLELAALPINPLWAAQVEPDCARRWALTGGDDYQLCFTLAPDQTPPL
                     AATAIGVITEALGIIGCEQGHEFALTGGGFEHFR"
     gene            2355..2783
                     /locus_tag="PKZ52_13765"
     CDS             2355..2783
                     /locus_tag="PKZ52_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNG60886.1"
                     /translation="MAFGFGSGLLPKAPGTWGTLAALPFYFLLNALPNYAYWMGLVLA
                     FAIGVWICDKTSNDIGEDDHGGIVWDEFVGLWLALACVPPGWGWVVAGFVAFRLFDAL
                     KPWPISLADKHVSGGLGVMFDDILAGVAALGSLKILEWLC"
     gene            2783..3481
                     /locus_tag="PKZ52_13770"
     CDS             2783..3481
                     /locus_tag="PKZ52_13770"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNG60887.1"
                     /translation="MRRLLTGLCCSGFLLGATAVWADADDDIVPLNAAKKNAAPIEVV
                     PLGLFNNSAVLKINGHERILRQGKTSPEGVTLISANARSAEVDINGQRQTLILNRGVG
                     TRFSNPPVAEARIASQVNGHYFTPGRINNQNVEFLVDTGATTITINANTAGKLGLEYD
                     KDKSVDVQTAQGMSKAYPVVLNSASVGNVTLNNIEALILEGEYPTAILLGNSFLSRVD
                     LKVEQGVMVLQSKY"
     gene            3520..>3749
                     /locus_tag="PKZ52_13775"
     CDS             3520..>3749
                     /locus_tag="PKZ52_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNG60888.1"
                     /translation="MSVEFVESCKLPTPFGMFVMHGFFDHTHQKEHVVLTLGDIKPEQ
                     TVLVRIHSECLTGDGLFSLRCDCGAQLQSAMY"
BASE COUNT          819 a         1051 c         1043 g          836 t
ORIGIN      
        1 aatgtttttt gaggtggaga ctggcaatat tgggggagtc catatacgga aaagccgcag
       61 gcgccttccg acctggatga gcagcctagg ttttacagtt agggaacctc tgaaaaatgt
      121 aggcggcgaa gcgctggcta ggcgaatttg ccaaaaaaag cggagtttac acgcagtaaa
      181 tgagcatttt tttggcaaat tcaacgacgc cagggcgagt aaggtaattt ttcagaggtt
      241 ccttagctgt cacaggcaat ttacgtcgga agtcgctgcg catttgcaac caaccctcaa
      301 ccgcattcag cgcctaacca atagaaaata gcccaagagg cgtcagcgtt ctgggcaact
      361 aaaccttttt aacgagagaa caacatgaac aaaattgaag gtgattttaa acaaagccaa
      421 ggccgctacg ccctgttagt gagccgctgg aacagctttg tggtggagca tcttaaagac
      481 ggcgccattg atacactgca gcgccacggc atccccgacg ccaatatcac cctggtctac
      541 gcccccggcg cttttgaatt tccgctggcc gcacaaaaac tcgcccgctc cggcaagttc
      601 gacgccatta tcgccctcgg cgccgtgatt cgcggcagca cgccgcactt tgattacgtg
      661 gccggcgagt gcaccaaggg cttagcccag gtgtctttgg atgcgggcat tcctgtcagt
      721 ttcggcgtgt taacggtgga ttccatcgag caggccatcg agcgctccgg caccaaagca
      781 ggcaataaag gcgccgaagc ggcagcaacg gccctggaga tggtgtccct gctcagcaaa
      841 atttaaccca gccgtttttt gttttagccc gcctaaggcg ggcattattt tttaagcatc
      901 cgagataacg cccatgccct ctacacccgc tgcccgcagc aaagcccgcc attacgccat
      961 gcaagccctc tatcaatggc agatgaccaa aaaccccatt aacaacatcc tggcggagtt
     1021 ccacgcggac aacgatatga acgtagtgga tgtgccttat tttcatgagc tggtgcacgg
     1081 cgtaccggcg gaaatcacca ccctggaaac cgacttctta ccctttgtta acgatatgag
     1141 cctggcgcaa atcgacccca tcaccctggc gattttgcgt atggcctgtt ttgagctgcg
     1201 ccaccgcatc gacgtgccct accgcgtagt aattaacgaa tcgttaaacc tggcgaaaaa
     1261 atttggcgcc acggatagtc ataaatttat caacggtgtg ttggacaaag tggcgggccg
     1321 cttgcgcagc gttgaagtta acgcgggcaa aaactaatta gtgaacgaat ttaccctgat
     1381 tcagcagtac ttcgccgcgc ttacgcctcc ctgcgccagt gtgccgcagg ccataggtga
     1441 tgactgcgcg ctgctggctc cgcccgaggg taagctactt gcagtgtcca tcgataccca
     1501 agtagaaggg gtgcactttc ccgttggcgc ggctgcagaa cagatcggtc gccgagttgc
     1561 ctgttgtgcc ctcagcgacc tggctgccat gggcgcgcag cccctctggg caacccttgc
     1621 acttacgttg cccaaagccg atgcaacatg gctggcagct tttagccggg gcttgggagc
     1681 ggtattaaac acctggcagg tgcgcctggt gggcggcgat accacttgcg gcccgctgac
     1741 catcagcctg caggtacatg gcagtgtggc cagtgagcac gcgctgcgcc gcagtggcgc
     1801 aagggcaggg gattgcatct atgtgactgg ccccttaggt gacggcgccg caggcctggc
     1861 ccttttgcaa ggccagttaa gcctgcccgc tgaggcagcc gcctatctca ccgagcgctt
     1921 ttacgcgccc acaccgcaaa tagctgcggg gcttgcgctt gcaggccgcg ctacgagcgc
     1981 catcgacatc tccgatggcc tgctcgccga tcttaaccac atcctcaccg caagccaggt
     2041 gggggcgcgc ctggagcttg ccgccctgcc gatcaacccg ctgtgggcgg cccaggtgga
     2101 acctgactgc gcccgccgct gggccctaac cggcggcgat gattatcagc tgtgttttac
     2161 actggccccc gatcaaaccc cgccgctagc cgctaccgcc ataggcgtga tcaccgaggc
     2221 gctgggcatc atcggctgcg agcaggggca tgaatttgcc ctcacaggag gtggctttga
     2281 gcactttcgt tgaaccgcca gcggcagcga tcaagcccga ctgggccttt atcagcgcta
     2341 aaccctggcg ctttatggcc tttgggtttg gctccggcct gttgcccaaa gctcctggca
     2401 cctggggcac actggcggca ctgccgtttt actttctctt aaacgcctta cccaactatg
     2461 cttattggat ggggctggtg ctggcatttg cgattggtgt gtggatctgc gacaaaacca
     2521 gcaacgatat aggcgaggac gaccatggcg gcatagtctg ggatgagttt gtcggcctgt
     2581 ggttggcgct ggcctgtgtg ccccctggat ggggttgggt cgtggcgggg tttgtcgcct
     2641 ttcgcctgtt tgatgccctc aaaccctggc ccattagcct ggcggataaa cacgtgtccg
     2701 gcggattagg cgtgatgttt gacgatattc tggcgggagt ggcagctctt ggcagtctta
     2761 aaattctgga gtggttatgc tgatgcgacg cttgttaact ggactttgct gcagcgggtt
     2821 tttacttggg gctactgcgg tctgggccga tgcagacgac gatatagtgc ccttaaacgc
     2881 cgccaagaaa aacgccgccc ccatcgaagt ggtgcccctt gggttgttca acaactccgc
     2941 cgtactgaaa attaacggcc atgaacgcat cttgcggcag ggcaaaacct caccggaggg
     3001 cgttacctta atctccgcca acgcccgcag cgccgaagtg gacatcaacg gccagcggca
     3061 gaccctaatt ctcaatcgcg gcgtaggtac tcgttttagc aacccgccgg tggccgaggc
     3121 gcgcatcgcc agccaggtca acggccacta tttcacgccg ggtcgtatca acaatcaaaa
     3181 cgtcgaattt ttagtggata ccggtgccac gaccatcacc attaatgcta atacggcggg
     3241 taaactcggg ttggaatacg acaaagacaa atcggtggac gtacaaaccg cccagggcat
     3301 gagcaaggct tatccggtgg tgttaaattc tgcctcggtg ggcaatgtca ccctgaataa
     3361 tatcgaggcg ttgattttag aaggcgaata ccccaccgcc attttgttgg gcaacagctt
     3421 tttaagccgc gtcgatttaa aggtagagca gggcgttatg gttctgcaga gcaaatatta
     3481 aataccgcgt ccatgcggta aatccctata ggtactgttg tgtcggttga atttgtagaa
     3541 tcctgtaaat tacccacgcc gtttggcatg tttgttatgc acgggttttt cgaccacacc
     3601 catcaaaaag aacatgttgt gctcactctg ggtgatataa aacccgagca aacggtttta
     3661 gtgcgcatcc attcagagtg cctaaccggc gatggcttat ttagcctgcg ctgcgattgc
     3721 ggtgcgcaat tacaatcggc catgtataa
//
LOCUS       DAOQGS010000411         3747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13754_length_3747_cov_4.159534, whole genome shotgun sequence.
ACCESSION   DAOQGS010000411 DAOQGS010000000
VERSION     DAOQGS010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13754_length_3747_cov_4.159534"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            600..2018
                     /locus_tag="PKZ52_13780"
     CDS             600..2018
                     /locus_tag="PKZ52_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNG60889.1"
                     /translation="MHNIKWNQPLGWFAAAIVSSALVGCGAAPAASSSSSSSSSSSSS
                     SSSSNSSSSSSSSNSSSSSSSSSSSSSSSSSSGGISLPLVVAIDSGSTTSTTLNGVVY
                     QADKYSTGGTANTTTDAVSGGALYSTERYGAFSYEVPVTANGRYTVVLHFAEIYQTTA
                     GARSFNVLIEGNSVASNLDIYSKVGHDVGYSVTYDDIPVVDGKVSIELVKGVENPTIA
                     GFAIYSRDGKLDTSVVTSPTANLTKYSAYVGKSTEKKTMTSNHATGLIGDYFFTHWKD
                     GGSASLTLDPTGVFSVSWQGGGYNYVGGPGWHYGDKNRVVGYRLDKDSGASYISLYGW
                     GYDKNLPTSNTARLVEYYILQRWTYDPSQGATFGKTFTSNGVQYSTYRSTRTSQPSIN
                     GRQTFYQYWSKPNQQQPLGTDNKIIFADHVKAWADSGWTLPDTNNTDASDDPTYQVLA
                     VEVFNPGNSGTGSGRVWDATPQ"
     gene            2396..3220
                     /locus_tag="PKZ52_13785"
     CDS             2396..3220
                     /locus_tag="PKZ52_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNG60890.1"
                     /translation="MKDKSGKITLTTIAMSWLIGCAPVAQSPAASQESGKTSTSVGIE
                     KFQAYTQKFNQKTLITQNHATGHIGNLFFTHWKDSGAASLNMDTDGAFSVSWQGGGYN
                     YVGGPGWHYGDKNRVIGYRFNEDSGASYITLYGWGYDKTMPTTNPAHLVEYYILQRWT
                     YDPSKDGVYGKTFVSNGVEYSTYRSIREQKPSINGITTFYQYWSKPTQQRALGQDHTI
                     VFADHLKAWAESGWVLPNTNNFDASDDPTYQVMAVEVFNPPKDGKASGKVWDATPH"
     gene            complement(3300..>3747)
                     /locus_tag="PKZ52_13790"
     CDS             complement(3300..>3747)
                     /locus_tag="PKZ52_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNG60891.1"
                     /translation="GISMVRQWTNESFASKADSAFAKIQAALPPNLQGEIQQITTYAV
                     PTLPPVPRTVSFSNLRNCIRTRQKISIEYCDQAKNSTTRTIWPLGLVFFNPVWLLISW
                     CETRKSFRNFRLDRIHSLSMSEAHFTDQPDKNLTAYLNTLHACQAQ"
BASE COUNT         1009 a          819 c          874 g         1045 t
ORIGIN      
        1 gctgcattca cctgtagtca ccactgctac tccgcgaatc gtttatgcgg tatcgccaaa
       61 tcctccagaa ttagccgccc gcacggaaga cggtaattag gtgtgctgaa tagttacaat
      121 tatttttatc gctcctgatg caatatataa caccctatta ttaactcggc actaaatgcc
      181 gccgggttaa aatctagcat tcacttgaac ctgtgggacc catttaacta tacgattctt
      241 ttttcaacat ttgcgtgccg aatggttgcg actctacatt tccgtttacg gttaaagcaa
      301 agatatagtc tgttttactc taatgtttta gggggaggta ttcggcattt ttttaacgct
      361 taagctgtat accggcaaat aaaataccgc acaccctagt ggttattaat taaatactga
      421 tcacgcaata gatgtgacct gattccatat gcccattggg ttaggttaat aaattttctc
      481 attggcgtgt ttcccagtgg agtatgactt aggccacaat tttttatgtt ggaatcagtt
      541 agcgtgtagc ctgacgaatt tgtattacct aaatcaacaa taaaattagg aaggcgttta
      601 tgcacaacat aaagtggaat caaccattag gctggtttgc cgcggcaatt gtatcgagtg
      661 ctttggtggg ctgtggcgca gcgccagcag cctcttctag cagtagctca agcagcagtt
      721 caagtagttc aagttcctcc tccaacagct ccagttcatc ttccagtagc aatagcagct
      781 catccagctc aagttcctcc tcatcctcta gcagctctag ttcttcaggc ggtattagcc
      841 ttcctttggt ggtcgcaatt gattccggca gtaccacaag caccactctt aatggcgtcg
      901 tgtatcaagc ggataaatat tccactggtg gcacagcaaa taccacaacg gacgcagtaa
      961 gtggcggtgc gctgtattca acggagcgtt acggggcgtt tagttatgaa gtaccggtga
     1021 cggccaatgg ccgatacacc gtggtgctgc attttgcaga aatttatcaa accaccgcag
     1081 gtgcgcgcag ttttaatgta ttaattgaag gcaatagtgt tgcaagcaat ttggatattt
     1141 attccaaagt aggccacgac gtgggttact cggtcaccta cgacgatatt ccagttgtag
     1201 atggtaaagt aagcattgaa ttggttaagg gggtggaaaa cccaacaatt gctgggtttg
     1261 ctatttattc gcgagacggc aaactcgaca catccgtggt gacgtcccca accgcaaacc
     1321 taactaaata ctctgcctat gtaggtaaat ccaccgagaa aaaaaccatg acttcaaacc
     1381 acgccacagg cttgattggg gattatttct ttactcattg gaaagatggt ggctctgcgt
     1441 cgttaacgct agatccaact ggtgttttct ccgttagttg gcaaggcgga ggttacaatt
     1501 atgtaggcgg ccctggctgg cactatggcg ataagaaccg agttgtaggg taccggcttg
     1561 ataaagacag tggcgcaagt tacatatctc tctatggatg gggttacgat aaaaaccttc
     1621 caacctccaa taccgcgcgt ttagttgaat attatatttt gcagcgctgg acctatgatc
     1681 caagccaagg ggcaacattt ggcaaaacat tcactagcaa tggcgtgcag tattctacct
     1741 atcgttcaac ccgtactagt cagccgtcaa ttaatggccg tcagactttc tatcagtact
     1801 ggagtaagcc caatcaacaa caacctcttg gaactgataa caaaattatt tttgctgatc
     1861 acgtaaaagc ctgggctgat tcggggtgga ctttgccgga tacgaataat accgatgcta
     1921 gtgatgatcc tacttatcag gtgcttgcag tagaagtatt taacccaggt aatagcggta
     1981 cagggtctgg ccgtgtgtgg gatgcaacac cacaataagc cataatcttc gggcgattaa
     2041 acccttatag ccattcaacc ttagtagctt atctttacag aggcgcacac cttccaggtg
     2101 ggcgcctttt tttcgctcct agaatcttta ggcttatacc ggttgttgac gcccagttgt
     2161 cgtagccatg ctagcctgtc cgactgaaca gatgatatgg gtctataatc catgaaacct
     2221 cgcaaactcc gtgcatccat agctatgcat cactggggct tattgaggac agtgaacagt
     2281 ctgaatccaa gcgcagtgat gagtttgccc agtggattta ataatgcgat aaattcctgc
     2341 tgagcttggt ttagaatttt gaatataagc gggttaatta atgaagagtg gcactatgaa
     2401 agataagtct ggcaagatta ctttaactac tattgcaatg agttggttaa ttggttgtgc
     2461 tccggttgct caatcgccgg cggctagtca ggagtcgggc aaaacatcaa ccagtgtggg
     2521 tattgaaaaa tttcaggcct acacgcagaa atttaatcaa aaaaccctga ttactcaaaa
     2581 ccacgccaca ggccatatag gtaatttatt ttttacacat tggaaggaca gcggggccgc
     2641 ttcactaaat atggatactg acggcgcctt tagcgtgagt tggcagggcg gtggttataa
     2701 ttatgtgggt gggccaggtt ggcattatgg cgataaaaat cgtgtcatcg gctatagatt
     2761 taacgaagat agcggtgcca gttatatcac tttatatggt tggggttacg ataaaactat
     2821 gcctacaacc aatccagcac accttgtgga atattatatt ttacagcgct ggacctatga
     2881 ccccagcaaa gatggcgttt atggtaaaac ctttgtgagt aatggcgttg agtattccac
     2941 ctatcgttcc attcgcgaac aaaaaccctc tattaacggt attaccactt tttaccaata
     3001 ttggagcaag ccaactcagc agcgagcgct cggtcaggat cacaccattg tatttgccga
     3061 tcacctaaaa gcttgggcgg aatccggttg ggtgttgcca aatacaaata actttgatgc
     3121 cagtgacgac ccaacttatc aggtaatggc cgtggaagta tttaatccac ccaaagacgg
     3181 caaagcctcc ggtaaggtgt gggacgcaac accccattaa ccctgcataa aaatccgccg
     3241 ggttaatgtt tggggtggat attgtgaagt aaaaggccac tcgctagcgg tggccttttt
     3301 tattgggcct ggcaagcgtg cagcgtgttt aaataagcgg tgaggttttt atcgggttgg
     3361 tcggtaaaat gcgcttcact catgctgagc gagtggattc tatcgagcct aaaatttcta
     3421 aaacttttgc gtgtttcaca ccaactgatt agcagccaaa ctgggttaaa aaacactaaa
     3481 cctaagggcc aaatcgtacg cgttgtggag tttttagctt gatcacagta ttcaatagat
     3541 attttttgcc gagtgcgaat gcaattgcgt aaattggaaa aactcaccgt cctgggcacg
     3601 gggggtaatg tcggtacagc ataggtagtg atttgttgaa tttcaccctg cagattcggc
     3661 ggtaaagccg cttgaatctt ggcaaatgcg ctgtctgctt tactggcaaa tgattcattt
     3721 gtccattggc gcaccatgct gatgccc
//
LOCUS       DAOQGS010000412         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13826_length_3736_cov_4.677533, whole genome shotgun sequence.
ACCESSION   DAOQGS010000412 DAOQGS010000000
VERSION     DAOQGS010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13826_length_3736_cov_4.677533"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3528)
                     /locus_tag="PKZ52_13795"
     CDS             complement(<1..3528)
                     /locus_tag="PKZ52_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nitrate reductase/sulfite reductase
                     flavoprotein subunit alpha"
                     /protein_id="HNG60892.1"
                     /translation="MSQTPTTAPSKTVASVCPYCGVGCGMLMEVADNKVVKLSGNKQH
                     PANFGRLCTKGSSSHQVLGHSGRLDKAYLRTSRSQASVSAPMAQAIAHVACELNRIRT
                     TYGPGAISFYVSGQMSTEAQYLANKLAKGFIGTNLIESNSRLCMASAGAGYKLSLGSD
                     APPGSYQDFEATNLFFVIGSNMADCHPILYLRMMDRVKAGAKLIVVDPRRTLTAEKAD
                     LFLHIKPGTDLALLNGILYLLHKAGRTDTAFIERYTQGWQAVEAMLPDYTPEAVSRVT
                     GLRVEDIQAAADLIAAAPEFMTCWTMGLNQSTHGTWNTNAICNLHLATGTICRPGSGP
                     FSLTGQPNAMGGREMGYMGPGLPGQRSLLVESERAEVEAIWGLAPGSLGTQLGKGTID
                     LFEQMVAGEVKACWIICTNPVASVPNRQTVIDGLKAAELVIAQDAFLDTETNAYADVL
                     LPGALWAEAEGTMINSERNITLMQPAVNPPGEAMADWRIIAEVAKAMGFSEGFNFASA
                     EEVFEEIKRFYNPKTGWDLRGVSFTRLRESPLQWPMPPEQTSSRSPLRYINDGRSQPL
                     HQDERGERPALVFATASGKAQFHARPHLAPAEMPCADYPLMLNTGRVQHQWHTLTKTG
                     KIATLNKLNPGTFIEINPQDAAALGINNRDSVQINSRRGSAILPAVITDKVVQGSCFA
                     PIHWNDVFGENLCINAVTSDYVDPISQQPELKVCAVALQKITLIKTLEITDPVSEPAH
                     LNHLATALVGALRPAPVFNETEQWYLSGLLEGLKPPPAPTEVPVIPPSAPFSSEKRYW
                     LDGLLAGLFSRTAQQLSAQPSPSTAPTLHLLFGSQTGNAETLASTCAAQFTRDGWNVI
                     VSSMDDYNTAQLAEQSLLIVITSTFGDGDAPDNAQNFWHFLSSVAAPTLHHLKFTVLA
                     LGDSNYDQFCGYGKKLDARLLELGAEHLLGRVECDSDYQQPFEQWLENVAAVLAPLKP
                     HLVPAAVQAADAKKLVSNAPALFSKNNPHAAKLITNQKLNGPGSNKDVRLFGFSLAEG
                     LNYEAGDALGVMPKNDVVLVNELLAQLNLPAHTDVSVPNCGSMALSDALIQHYEIGKP
                     HPDTLKFIAERSSNTDLKTLLNGDKNSLKHWLYGRQLIDLLREFPVAASAEEWLLQLK
                     KLQPRLYSIASSPKAYGDQVQLCVSAVRYG"
BASE COUNT          767 a          948 c         1145 g          876 t
ORIGIN      
        1 gccataacgc accgccgata cacacagctg cacttgatcg ccataggctt ttgggctgga
       61 ggcgatggaa taaagcctgg gttgcaattt ttttaactgc agcagccact cttctgcact
      121 tgccgcaaca gggaattccc gcagcaggtc aattaattgc cggccgtaca gccaatgttt
      181 taagctgttt ttatcgccat ttaagagggt ttttaaatct gtattgcttg agcgctcggc
      241 gatgaatttt agggtgtccg ggtggggttt gccgatttcg tagtgctgaa ttaacgcatc
      301 gcttaaggcc atggagccgc agttgggcac gcttacatct gtgtgggcag gcaaatttaa
      361 ttgtgccaat aattcattga ccaaaaccac atcgtttttg ggcatcacgc ctagggcgtc
      421 accggcctcg taatttaggc cttccgctaa ggaaaaacca aataaacgca cgtctttatt
      481 agagcctggg ccgttgagtt tttgattggt aattaatttg gcggcatggg ggttattttt
      541 gctaaataat gcaggcgcat tcgaaactag ttttttggca tccgccgctt gcacagcggc
      601 gggtacaagg tgcggtttta agggtgctaa aactgccgct acattctcca accattgctc
      661 gaagggttgt tgataatcgc tgtcgcactc tacccggccc aatagatgtt cggcgccaag
      721 ttctaacagg cgggcatcga gttttttgcc atagccgcag aattgatcgt aattggaatc
      781 ccctaaggcc aacacggtga attttaaatg gtgcagtgtg ggcgcggcca cggagcttaa
      841 aaaatgccaa aaattctgcg cattatccgg cgcgtcgcca tcgccgaagg tgctggtaat
      901 tacaattaat aatgactgct cggccaattg ggcggtgttg taatcatcca tgctgcttac
      961 aattacattc cagccatcgc gggtgaattg cgcggcgcag gtgctggcga gggtttccgc
     1021 attgccggtt tgcgagccga acaacaggtg cagggtgggg gcggtggacg gacttggttg
     1081 agcgctcaat tgctgtgctg tgcggctaaa taatccggct aaaaggccat ccagccagta
     1141 gcgtttttcg ctgctaaagg gtgcggaagg cggaattact ggcacttcag tgggtgccgg
     1201 ggggggctta agcccttcta ataaaccact taaataccac tgctcggttt cattaaatac
     1261 cggcgctggg cgcagggcgc ctaccaaagc tgtggctaag tgatttaggt gtgcaggctc
     1321 actcacaggg tcagttatct ctagggtttt aatcagcgta attttttgca acgcaaccgc
     1381 gcagactttt aattccggct gttgggatat tgggtcaaca taatcgctgg tgacggcgtt
     1441 aatacagagg ttttcgccga atacatcatt ccaatgaata ggcgcaaaac agctgccctg
     1501 caccactttg tcggtaataa ctgcaggcaa aatagcgctg ccccggcggg aatttatctg
     1561 cacagaatcc ctattattaa tgcctagggc ggcggcatct tgcgggttaa tttcgataaa
     1621 ggtgccgggg tttaatttat tgagggtggc gatcttgccg gttttagtaa gtgtgtgcca
     1681 ttggtgctgc acccggccgg tgttgagcat caacgggtag tccgcgcagg gcatttccgc
     1741 cggagccagg tgggggcgag cgtgaaactg tgctttgccg ctggcggtag cgaataccaa
     1801 ggccgggcgc tcgccgcgct catcttgatg caagggctgg ctgcggccgt cattgatgta
     1861 gcgcaggggg ctgcgggagc tggtttgctc agggggcatg ggccactgca ggggtgattc
     1921 gcgcaggcgc gtaaaactca cgccgcgtaa atcccagccg gttttggggt tgtaaaagcg
     1981 tttaatttcc tcgaatacct cttcggcact ggcgaagtta aagccttcgc taaagcccat
     2041 ggctttggcc acttcggcga tgatgcgcca gtccgccatg gcctcgccgg gcgggtttac
     2101 cgccggttgc atgagggtga tattgcgctc ggagttgatc atggtgcctt cagcctcggc
     2161 ccagagggcg ccgggcaaca gcacatcggc gtaggcgttg gtttcggtat cgagaaaggc
     2221 gtcctgggcg ataaccagtt cagccgcttt taaaccatca attaccgtct gccggttggg
     2281 tacgctggcc accgggttgg tgcagatgat ccagcaggct tttacttccc ccgccaccat
     2341 ctgctcgaac aggtcgatgg tgcctttgcc cagttgggtg cctagactgc cgggggccag
     2401 gccccagatg gcttccactt ccgcccgctc cgactccacc aagaggctgc gctggcccgg
     2461 cagccccggc cccatataac ccatctcgcg gccgcccatg gcattgggtt ggccggtgag
     2521 ggaaaagggg ccggagcctg ggcggcagat ggtgccggtg gccaggtgca ggttgcaaat
     2581 ggcgttggta ttccaggtgc cgtgggtact ttggttgagg cccatggtcc agcaggtcat
     2641 aaactcgggg gcggcggcga tcagatcggc ggcggcttga atgtcttcca cccgcaggcc
     2701 agtaacacgg ctcaccgcct cgggggtata gtccggcagc atggcttcaa ctgcctgcca
     2761 gccctgggtg tagcgctcga taaaagcggt atcggtgcgg ccggctttgt gcagcaggta
     2821 caaaatgcca ttgaggagcg ccaggtcggt gccgggtttg atgtgcagaa agaggtcggc
     2881 cttttccgcc gtaagggtgc ggcgggggtc caccacaatt aacttagccc cggcttttac
     2941 ccgatccatc atgcgcaggt acaaaatcgg gtggcagtcc gccatattgg agccgatgac
     3001 gaagaaaaga ttagtggctt caaaatcctg gtaggagccc ggcggcgcgt cggagccaag
     3061 cgataacttg taaccggccc cagcgctggc catacacagg cgggagttgg actcgattaa
     3121 gttggtgccg ataaacccct tggcgagttt gttagccaag tactgagcct cggtggacat
     3181 ttggccggag acataaaaac tgatggcgcc ggggccatag gtggtgcgga tgcggttgag
     3241 ttcgcaggct acatgggcga tggcctgggc catgggggca gacactgagg cttggctgcg
     3301 ggatgtgcgc agataggctt tatccaggcg gccggagtgg ccgagcactt ggtgactgct
     3361 ggagcctttg gtacagaggc ggccgaaatt ggcggggtgt tgtttgttgc cgctgagctt
     3421 caccaccttg ttgtccgcca cctccatcaa catgccgcag ccgacgccgc aataggggca
     3481 cacgctcgcg acggttttgc tcggtgcggt tgtgggtgtc tggctcacat caaattcgct
     3541 cttaggtcgt gaagctggct tagcaaggcc tatgccacgg ccccaagagc gccataaggg
     3601 cggtctatcc acatgaattc aaaagggttt ctgatttgat aggcgggcgt ttagggccgc
     3661 cactgcggtg gtgtgcactc ggctgcgccc cataaaggca ccacaaaaga tcgtcaaggc
     3721 accaaattag agtctt
//
LOCUS       DAOQGS010000413         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13857_length_3730_cov_4.429660, whole genome shotgun sequence.
ACCESSION   DAOQGS010000413 DAOQGS010000000
VERSION     DAOQGS010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13857_length_3730_cov_4.429660"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3349)
                     /locus_tag="PKZ52_13800"
     CDS             complement(<1..3349)
                     /locus_tag="PKZ52_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60893.1"
                     /translation="MLAAGVDQKTELHKSTTSKVALSKHTGDLTLGANSLTQVSLEQP
                     PQAVLVDDASLSAAAVAQLKSLAGSKSVDIKDAAGQAFKGLAVTPAAYAQWQQGQSDS
                     PRRVRVLSAEGYIDQLAADRIQGATAVDASVQNPSKIELYGKHIELGEDAQVAAKGGA
                     IKLVATDGVEPMKSTRSDSVDASVHFAEGASLDVSGAQVNLAMERNSLEVEVRGNEVR
                     DQPLQRDGVLRGQKVRVDARLGTSMVDTKGSQEKQAKSVAERSTQGGSVQVLSEGAVD
                     FDPNSQVNIKGGKVNYSGGELVTTGLIKDGKVVDISAADPNQVYEGLQTLSRYEEGYT
                     DVQDAGRLEIKARNLQLDANVLAGQAPGQRQLDIKSQPKQGSLLLDLNYFDVDANNIQ
                     KLTGQSVWLSPLRPEGMDLKRETWLNPQLINAASLSELQLFLRGDLTQERGGDIHLAP
                     GGNFTAQAQEILLAGTIDASGGNIDISSQPAVGRGQFSNSTVVQKDEAQHTVQLLAGA
                     RLDVSGTWVNRSASVEQGQQAVSVLDGGSVKLSSRGDLILDAGATIAAEAGALVSASN
                     AFTGGKGGAINLKAAFPATQVKLTLEADLLARGFSQNGSLSINLPALQIGQGNAVQKD
                     MAAKVFGYDFFAEGGFSKYSFTTNLGGVDILPQEGASFAWQQRNWVMKDSAYLAAQSS
                     RSVAALAASQLLPDYLRKPVSINIAQNRPKSFFGLELESFDKYLHFSDGVDWRFDPLA
                     KVDFASIKNMYVGGSIYAPGGELSFNNNRQSNNLFSYDPSSTLWLGKEAWLSVAGTFI
                     PSPGEPNLSLGKLINGGRIRFGLSPLDEAYTNGATPTGRIVSEEGSGIDLSAFSATFD
                     VLGGDGYRKESRASDAGSLEFYIADGLLWQSNVLAKSFRELGAKGASVSLTLLPEGDL
                     RSYNNDQKFPDSKRGFDFFAQRQQDLLEGMSFGDDLPVNISKIDGDVAPSFNRVFLFA
                     DDINRWNLDELSLDSIDSVLRYSGAADKELSADIHLRDSFELTVKSSLNLDAREIAVA
                     EVARAELYAPWLVLGEQTYVSKVETKQNDIDQQVKPIKPFRETLPAQGYLTLFGEQVE
                     LAGDLQLNGAAQFEREVTGERIRDKIAA"
     gene            complement(3262..>3730)
                     /locus_tag="PKZ52_13805"
     CDS             complement(3262..>3730)
                     /locus_tag="PKZ52_13805"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNG60894.1"
                     /translation="PPTRLPFTLVDGVNGNRVGGAGTPLDPRLGPLQNNGGATLTYLL
                     LPSSPARDAGDPGFDGAGLTDQRGAARVVNGRVDIGALEAATGLLVYYPFDNFNPADA
                     LGSSSLTYQGDGGVWWNTDHRGQGSCLRRVLIRRRNCINPQPVKLRSPNIPAI"
BASE COUNT          845 a         1105 c          904 g          876 t
ORIGIN      
        1 tggcggcaat cttgtcgcgg atgcgctcgc cagttacttc gcgttcgaac tgggctgcgc
       61 catttaattg aagatcacct gcaagctcaa cctgctcgcc aaataatgtt aaataaccct
      121 gagctggaag tgtctcccta aagggtttaa ttggcttgac ctgttgatca atatcatttt
      181 gtttcgtctc aactttactt acgtaagttt gttcgcccaa aaccagccaa ggcgcataaa
      241 gttctgcccg ggcaacttcc gctactgcaa tttcccttgc atcaagatta agagatgatt
      301 ttactgttaa ttcaaacgaa tcacgcagat gaatatctgc actcagttct ttgtcagcag
      361 cgccagagta tctcagcacg ctatcaattg aatctaagct caattcgtcc aaattccagc
      421 ggtttatatc atcggcaaat agaaagacac gattgaagga gggggcaaca tcaccatcta
      481 ttttagaaat atttaccggc aagtcatccc caaaactcat accttctaac aaatcttgtt
      541 ggcgctgtgc aaaaaaatca aaaccgcgct ttgagtcggg aaatttttgg tcattgttat
      601 aagaacgcaa atcgccttcg gggagcaaag taagacttac acttgcgcct ttagcgccca
      661 attcccgaaa actctttgcc aaaacattgc tctgccaaag caaaccgtca gcaatataaa
      721 actccaaact gcctgcgtcg cttgctcgac tttctttgcg atacccatct ccacccagca
      781 catcaaaagt agcgctgaat gcagataaat caattcctga gccctcctct gacacgatac
      841 gcccggtcgg agtagcacca ttggtataag cctcatccaa aggtgacaat ccaaatctga
      901 ttcgccctcc atttattaac ttccctaatg ataaattagg ctctccggga ctcgggataa
      961 atgttccagc aaccgacaac caagcctctt tgccaagcca aagtgttgaa cttggatcat
     1021 aggaaaataa attattagac tgacggttgt tattgaaact aagttcgcct cccggagcat
     1081 agatagagcc gccaacatac atgtttttaa tgctagcaaa atctactttc gctaggggat
     1141 caaaccgcca atcaactccg tcggaaaaat gaagatattt atcaaagctc tcaagctcca
     1201 agccaaaaaa actcttgggt ctgttttgag caatattaat actcaccggc ttacgcaaat
     1261 aatccggtaa caattggcta gcggctagtg ccgccactga gcgactcgat tgcgcagcca
     1321 agtaggcgct atccttcatg acccaattgc gctgttgcca tgcgaaactt gcaccctctt
     1381 gcggcaaaat atccacgccg cctaggtttg tggtgaagct gtatttacta aagccaccct
     1441 cggcaaaaaa atcgtagcca aacaccttag ctgccatgtc tttctgaacg gcattgccct
     1501 ggcctatttg cagggcgggc agattaatgc tgaggctgcc gttttggcta aagccacggg
     1561 ctaatagatc agcctcaagg gtaagcttca cctgtgtggc cggaaaggcc gcctttaaat
     1621 tgatcgctcc ccctttgccg ccggtaaatg cgttgctggc acttacaagg gctccggcct
     1681 cagcggctat tgtggcccca gcatccaaaa ttaggtcgcc tcggctactg agtttaaccg
     1741 agccaccatc caataccgat accgcctgtt gcccctgctc gactgaggct gaacgattga
     1801 cccaggttcc cgatacatcc agacgggccc ccgccaacag ttgcacagtg tgttgtgctt
     1861 cgtccttttg aacgacggtt gagttactaa attgcccgcg accgacagcg ggttggctgc
     1921 tgatatcaat gtttccgcca gaggcgtcga tggtgccagc caacagaatt tcctgggcct
     1981 gagcggtaaa attgccgcct ggggctagat gtatatcccc tccacgctct tgggttaaat
     2041 ccccccgcag gaacaactgc agctcggaga gactcgcggc attgatcagt tgcgggttca
     2101 accaagtttc ccgcttaaga tccatgccct ccggacgcag aggactcagc cacaccgatt
     2161 gcccggtaag cttttggatg ttgttggcgt caacgtcgaa ataattaaga tcgagcaaaa
     2221 ggctgccctg cttaggctgg ctttttatgt ccagctgccg ctgccccggc gcctgcccgg
     2281 ccagcacatt ggcatccagc tgcagattgc gcgccttaat ttccaaacgc cccgcatcct
     2341 gcacatcggt ataaccctct tcatagcggc tgagggtttg caggccttcg tacacctggt
     2401 tggggtccgc cgcgctgata tccactacct tgccgtcttt aattaggccc gtggtaacca
     2461 gttcaccgcc gctgtaattt accttgccgc ccttgatatt cacttgggag ttcgggtcaa
     2521 aatccacagc accttccgat aacacctgca ccgagccgcc ctgagtggag cgctcggcca
     2581 cgcttttggc ctgtttttcc tggctgcctt tggtgtccac catgctggtg cctaggcgcg
     2641 cgtccacccg caccttttgc ccgcgcagta caccatcccg ctgcaggggt tggtcgcgca
     2701 cctcgttgcc gcgcacttct acttccagag aattgcgctc catagccaga ttgacctgcg
     2761 cacccgacac atccagcgag gcgccctcgg caaaatgtac agacgcatcg accgaatcag
     2821 atcgtgtgga tttcatgggc tctaccccat cggtggcgac caacttaata gccccgccct
     2881 tagccgccac ctgagcgtcc tcacccagct ctatgtgttt gccgtacagc tcgattttgc
     2941 tggggttttg cacgctggca tccaccgccg tggcgccctg tatacgatcg gccgccaact
     3001 ggtctatgta gccttccgca ctcaatacgc gcactcggcg ggggctatca ctctgcccct
     3061 gctgccattg ggcgtaggcc gcaggcgtaa cggccaagcc cttaaaagcc tggcctgcgg
     3121 catctttaat atccacactt ttactaccgg cgagggactt aagctgagcg acagcggcgg
     3181 cacttaggga ggcgtcgtcc accaacacgg cctggggcgg ttgttccagg ctcacctggg
     3241 tgaggctatt ggcgcctagg gttagatcgc cggtatgttt ggagagcgca actttactgg
     3301 ttgtggattt atgcagttcc gtcttctgat caacacccgc cgcaagcaac tgccctgacc
     3361 gcggtggtcg gtattccacc acaccccgcc gtccccttga tacgtgagcg atgatgaacc
     3421 cagcgcgtcc gccggattga aattatcgaa tgggtaatac accaacaacc cggtcgccgc
     3481 ctcgagcgcg ccgatgtcca cccggccatt gacgacgcgc gccgcaccgc gttgatccgt
     3541 gaggccggcg ccgtcgaagc cgggatcgcc cgcgtcgcgc gccgggctgc tcggcagcag
     3601 caaatacgtg agcgtggcgc cgccgttgtt ttgcagcgga ccgaggcgag gatccagcgg
     3661 cgtgccggcg ccgccgaccc ggttgccatt cacgccgtca acgagcgtga atggcaaccg
     3721 ggtcggcggc
//
LOCUS       DAOQGS010000414         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13872_length_3727_cov_5.148420, whole genome shotgun sequence.
ACCESSION   DAOQGS010000414 DAOQGS010000000
VERSION     DAOQGS010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13872_length_3727_cov_5.148420"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PKZ52_13810"
     CDS             complement(<1..1428)
                     /locus_tag="PKZ52_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60895.1"
                     /translation="MSNQHRWKFSRIGGFDQALITSKEDLLSLEQLDQKLWAALSCPV
                     KGLQLDEAMLAAIDVDGDGRVRVPEIVAAVNWACSALKDPNLLLSSADSLNLSDFSDG
                     ELGQKLLASSKEILIALGKAEQTVISLQDAQSQEAIVANTRFNGDGVVPLAATDDEQL
                     QALLNLIMHTQGESTDLSGKPGINQDALAGFFKALNERLSWLDEAGPTVKPLSDNCEG
                     AYKALLAVQGKIDDFFSRTRLVAFDSRSQEQLNKPVSSLETLGSSDLSSACDEVKHFP
                     LALVSADAKLPLADGINPAWQAAMADFVAQVVTPLLGKDLTHLEFNQWQSLKATFNAY
                     QQWLAAEKGASLKGICADELRRWADPKWQAALEDLMQRDLAMAPAVKAFADVKKILLL
                     RLHLKKLLNNFVNFTEFYGSVGQAVFQAGTLYLDGRACELCIRVDDAAKHATLAGLSK
                     CFLAYCDCTRSDGAKMTIAAAFTAGD"
     gene            1513..1962
                     /locus_tag="PKZ52_13815"
     CDS             1513..1962
                     /locus_tag="PKZ52_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60896.1"
                     /translation="MLRLLLPILFPSWRFFNSIGPSPRLMLQFDAEPWLEYYPKPASL
                     PVREHLARLFLNPRGNKLLFVQSCAVRLFDEQDASAVENIFLALAQAIHFGQLVPPSP
                     EASLRWRVEQLQWETTGNLTAIPVYTSDARCVSELAALWQRDTRLCP"
     gene            1953..2771
                     /locus_tag="PKZ52_13820"
     CDS             1953..2771
                     /locus_tag="PKZ52_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60897.1"
                     /translation="MPISLGLLLSQLGLALAVFGQALEHQQASSGERRLAALRAWAAA
                     LLLVGIIAGFCCGALAQGAPWASHLWGTAQAFVTKQTLAPLALISQAVRALACWGILL
                     VGILSLKRFGGPYCGGSDLMTLQLSLAMAIAESTTSPAWRLGAAGYLCIQLCLSYWQS
                     GWVKLVNPQWRNGRALWEVFAFTHYPVTQATRRWAEHPRILWLMGWLIMLVEFCFPLS
                     LYYMASLYAGLVFMGLFHLANAWLFGLNRFFWIWLATYPTVIAFAPIIAEFMRR"
     gene            complement(3148..3438)
                     /locus_tag="PKZ52_13825"
     CDS             complement(3148..3438)
                     /locus_tag="PKZ52_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60898.1"
                     /translation="MKLVSASLVNIKRARREMERAFTAGNWEDVKRWDQLVGRGLDAA
                     FDDPNRDNAGLVEELEKVLGLYAQIVIGLPEQTAQQWLNAPISVGEGKREFK"
BASE COUNT          807 a         1055 c          951 g          914 t
ORIGIN      
        1 atcaccggcc gtaaaggcgg cggcgatggt cattttggca ccgtcactgc gggtgcaatc
       61 gcagtaggct aaaaaacact tgcttaaacc cgccagggtt gcgtgtttgg cggcgtcgtc
      121 cacgcggata cacagctcgc aggcgcggcc atccaaataa agggtgcccg cttggaatac
      181 cgcctggccc acggagccat aaaattcagt gaagttaaca aagttgttga gcagtttttt
      241 aagatgcagg cgcagcagca ggattttttt cacatcggcg aaggccttta ctgccggcgc
      301 catagccaag tcccgctgca ttaaatcctc caaagccgcc tgccacttag ggtccgccca
      361 gcggcgcagc tcatcggcgc agatgccctt gaggcttgca cccttctccg ccgccagcca
      421 ctgctggtag gcattaaagg tggctttaag gctctgccac tgattaaatt ccaagtgggt
      481 taggtctttg cccagcagag gcgttaccac ctgggcgaca aaatccgcca tggcggcctg
      541 ccaggccggg ttgatgccgt ccgccaaagg cagcttggca tcggcgctta ccagggcgag
      601 ggggaagtgt ttgacttcgt cgcaggcgga ggacaaatcg ctgctgccga gggtttccag
      661 gctcgagacc ggtttgttga gttgctcctg gctgcggcta tcaaaggcca ccaggcgggt
      721 gcggctgaag aagtcatcaa ttttgccctg caccgccaag agtgctttat aggctccctc
      781 gcagttgtcg cttaagggtt tgaccgttgg gcctgcttca tccagccagc ttaagcgctc
      841 gttgagggcc ttaaaaaatc cggccagggc gtcttggttg atgccgggtt tgccgcttaa
      901 atcggtgctt tcgccctggg tgtgcataat caggttaagc agcgcctgta attgttcatc
      961 gtctgtagcg gccaggggca ccacgccatc accgttaaag cgggtattgg ccactatggc
     1021 ttcctggctt tgggcgtctt gtaggctaat gacggtttgc tcagccttac ccagggcaat
     1081 aaggatttct ttactgctgg ccagcagttt ttgccccaac tcgccgtcgc taaagtcact
     1141 caggttcagg ctatcagcgc tgctcagcag caagttgggg tctttcagcg cactgcaggc
     1201 ccagttcacc gccgccacta tctccggcac ccgcacccgg ccatcgccat ccacatcaat
     1261 agccgccaac atagcctcgt ccaactgcag gccttttacc gggcagctca gggccgccca
     1321 gagtttttga tccagctgct caaggctgag cagatcctcc ttgctggtaa ttaaggcctg
     1381 atcaaaaccg cctatgcgac tgaatttcca tcggtgctga ttgctcatgg ataacctcgt
     1441 gttaagttga taattgcctt aggcgttaaa ttgcctgcct atactggggt aacaggttaa
     1501 acgagccccc ttatgctgcg cttactgctg cccattcttt ttccctcctg gcgttttttt
     1561 aacagcatcg ggccgagccc gcgtttaatg ctgcaatttg atgccgaacc ctggctggaa
     1621 tactacccta agcctgccag cttgcccgta cgcgagcacc tggccaggct gttcttaaac
     1681 cctcggggca acaaactctt attcgtgcaa agttgcgccg tgcgcctctt cgacgagcag
     1741 gatgccagcg ccgtggagaa tatttttctc gccctcgctc aagccattca ctttggccag
     1801 ttagtgccgc cttcgcccga ggcgagcctg cgctggcgag tcgagcaact gcagtgggaa
     1861 accacaggca atttaacggc catcccggta tacaccagcg acgcccgctg cgtgagcgag
     1921 ctggccgccc tctggcagag ggatacacgt ttatgcccat aagcctaggg ctactgttga
     1981 gccaactggg cctcgccctg gctgtttttg ggcaggcatt ggagcatcaa caggcctcaa
     2041 gcggtgagcg gcgcctagcc gccttacgcg cctgggccgc cgctttatta ctggtgggga
     2101 ttattgctgg attttgctgc ggcgcgctag cgcagggcgc cccttgggcc agccatttat
     2161 ggggcacagc gcaagctttt gttaccaaac aaacccttgc accccttgcc ctaatcagcc
     2221 aagccgttcg agcactcgcc tgttggggga ttttgctggt gggcatacta agccttaaac
     2281 gctttggcgg cccctattgc ggcggcagtg atcttatgac tctgcaatta agcctcgcca
     2341 tggccattgc cgaatccacc actagccccg cttggcgctt gggggcggcg ggctatctgt
     2401 gtattcagct gtgtttgtcc tactggcaat ccggctgggt aaaactggtg aatccccagt
     2461 ggcgcaatgg ccgcgcgctt tgggaagtgt ttgcctttac ccattatcct gttacccaag
     2521 ctacccggcg ctgggccgag cacccgcgca ttttatggct gatgggttgg ctgattatgc
     2581 tggtggaatt ttgttttccc ttaagcctgt attacatggc aagcctgtac gccgggttag
     2641 tctttatggg cttatttcat ttagccaatg cttggctgtt tggcttaaac cgttttttct
     2701 ggatttggct tgccacctat cctacggtga tagcctttgc gccaataatc gcggagttta
     2761 tgcggcggta aatgtgcctc aaactttaga caggaaaacg tataggttgg tgctcaaaga
     2821 attccgcctg gattgggccg aattataaat aacggagaag tacaataatt aaaaagacaa
     2881 taaaacccca cacattaaaa agggcgaacg cgacagcttc gcccccatta acaatctatc
     2941 taatcaagcg acagccaaca tacatcaaaa cctatcgcca gctatcaacc cggtggcaag
     3001 gatgccgcat caaaggtgca aacggccgca agctacacta aaacaagaaa atccgcgcat
     3061 tttcgtaagt ttagtgcagc gaaggtaaaa taaatcagga cgatttattt taccactggg
     3121 ttaataacac tgctgaaaaa ctgcaggtta tttaaattcc cgtttccctt ctcccactga
     3181 aataggtgca tttaaccact gttgcgcagt ttgttccggc agcccaatta caatttgtgc
     3241 atacaaaccc agcacttttt ccaactcttc aaccaagccc gcattatcgc gattggggtc
     3301 atcaaaagcg gcatcaagcc ccctgcccac caactgatcc cagcgcttaa cgtcttccca
     3361 attgccagcg gtaaaagctc gctccatctc cctgcgtgcg cgttttatat ttactaatga
     3421 tgccgatacg agtttcatac tgcgctcctg attgggtgat atcccgttta acggccagta
     3481 aacgggaaac tttaggtgcc agtgcggcgc aaaattacca ccccttgccc aggccaaaag
     3541 actcatgtgc attcggctac tttttgggag ctgaggcccc acggcctcta ggcgtcacaa
     3601 aaagttcatc tttttaacat cccagcttca tatagggccg cgacaatcgc cattgataca
     3661 tccaagccga cgatttacgc agggtcttca ctactatgca cgcaaaaacc tttggtattt
     3721 tttcatt
//
LOCUS       DAOQGS010000415         3721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_13915_length_3721_cov_4.394981, whole genome shotgun sequence.
ACCESSION   DAOQGS010000415 DAOQGS010000000
VERSION     DAOQGS010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13915_length_3721_cov_4.394981"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1150
                     /locus_tag="PKZ52_13830"
     CDS             287..1150
                     /locus_tag="PKZ52_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNG60899.1"
                     /translation="MHILLTGASGFIGSALSRALTQQGHTLVPVSRRHGVDFSRMQAA
                     GNWLPLLAGVDAVINAVGIIAEGGGQRFDVLHRHAPQALFNACQQAGVGKVIQISALG
                     ADERAVTAYHLSKRAADDALRRLPLDWFVLRPSLVYGQGGASSSLFMRLSRLPLVPLV
                     GSGEQLVQPVHISDLVATVLRCLAQKGGQQTLDVVGPEVVSFAQWLQQMRSSQGLGAA
                     RFVPIPAGLVMAACQLGRFISPLMQPDNLRMLQMGSTADVKPLQTFLGRSPLGVAPHL
                     FTRNEGAVYEL"
     gene            1140..1619
                     /locus_tag="PKZ52_13835"
     CDS             1140..1619
                     /locus_tag="PKZ52_13835"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HNG60900.1"
                     /translation="MSYELVKTLHILSMVLLFGTGLGSAFYKWMADRSGAVAHIAITN
                     QHVVLADWLFTTPTVIFQPLSGLWMVLHLGLPISTPWVMISLGLYALAGACWLPVVWL
                     QIRMRSLSAQAAAAEEALPAEYWALARMWFWLGVPAFIAMVLVVALMVFKHIPGVEL"
     gene            1616..2212
                     /locus_tag="PKZ52_13840"
     CDS             1616..2212
                     /locus_tag="PKZ52_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HNG60901.1"
                     /translation="MSSLMQSALGAQFEQLPPALKGHYRAGNTLEQGHLDIEYPRWMQ
                     PFLTLLRLLGALVNRSGPKVPTQVEKFDSPSGQRWRRTISFPDGKVVYFNSRWVPGQG
                     NRLIEFVNPMVGLEMAVHVANGKLYYEGVRFVVKLGSLLIPIPEWLLLGHTSIEEQGL
                     DEHHFAMDFRLTHPLFGQIFRYAGTFAIEPEPVVNRLE"
     gene            2237..2950
                     /gene="tsaA"
                     /locus_tag="PKZ52_13845"
     CDS             2237..2950
                     /gene="tsaA"
                     /locus_tag="PKZ52_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNG60902.1"
                     /translation="MAYTLAPIGIIHSCFSQKFGIPRQPGLAPAATGQLVLLPPFNNP
                     EAVAGLEGVSHLWVEFVFHAGYGGWKPRVKMPRLGGNKTLGVFATRSPNRPNNLGLSV
                     VSLLKVELEPQVRLTLGGVDMLDGTPVLDIKPYLPYADVIENARNDWAAEPPALIAVQ
                     FSPQAQAQFSELNARYPKLAELAAQVLSQDPRPSYQDYTSERTYALRLWDVDIHWRCV
                     AASTGFMIEVLFCRLVVPD"
     gene            2997..3620
                     /locus_tag="PKZ52_13850"
     CDS             2997..3620
                     /locus_tag="PKZ52_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014480914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNG60903.1"
                     /translation="MFKSLAADVLGLSDIGKILDKKDFDKADVDDYVFAEDGEHIYVV
                     IKSKTDEYCFTNLAMIHLDGSSAVSKKRLLHRYPYKHHPVSHVRIETAGTVDLDVELK
                     FKLADKEYSIDIDKTQIERVKNIYKALTVIGEKCREIHRQMETLRHTCAAVNGMFVLR
                     ELPEQVVLSLPDIINQTVLQVEAHYNERRKEIQNYDFGEVFGRYIKW"
BASE COUNT          784 a          969 c         1089 g          879 t
ORIGIN      
        1 cgctgaatat gggcgctttt ttgtcaaaat ttggcgaaag cagtagtcac cactagggtg
       61 agtcaaattt tgacaaaaaa gcgtgcagat tcaagtatta ggcagccgta gtagagttca
      121 gctgcggctt ttaggtttaa ctactcaaac cttcgtatgg atccatccgg agggcttttt
      181 tgttccgctc tgatcgcagc ggaagtattc ccgcgtacaa cctcccaaac tccagttgtg
      241 gtttcagcaa tcgtaagcaa cacaagagcc ttggaggcca agacgtatgc acatcctttt
      301 aaccggcgcc agcggattta tcggcagcgc cctaagccgt gcgctcacac agcagggcca
      361 taccctggtg ccggtcagcc gccgtcatgg agtggacttt agccgcatgc aggcggcggg
      421 caattggctg ccgctactgg caggggtgga cgcggtgatt aacgccgtgg gcatcatcgc
      481 cgaagggggc ggccagaggt ttgatgtatt gcaccgccac gccccccagg ctctttttaa
      541 cgcctgccag caggcagggg tgggcaaggt aatacagatt tccgccctgg gggctgatga
      601 gcgggcggtg acggcctatc acctgagtaa acgcgccgcc gatgacgccc tgcgccgcct
      661 gcccctcgac tggtttgtac tgcgcccctc cctggtgtac ggccaaggtg gtgccagtag
      721 tagtttgttt atgcgcctgt cccgcttacc cctggtgccc ctggtgggct ctggtgagca
      781 gctggtgcag ccggtgcata tcagcgattt agtcgcaacc gtactgcgct gcttggcgca
      841 gaagggcggc cagcagactt tagatgtggt ggggccagag gtagtgagct ttgcccagtg
      901 gctgcaacag atgcgcagca gccagggttt aggggcggcc cgttttgtac ctatccccgc
      961 cgggctggtg atggccgcct gtcagttggg gcggtttatc agcccactta tgcagccgga
     1021 caacctgcgt atgttgcaga tgggcagcac ggcggatgtt aaacccctgc agacgttttt
     1081 agggcgcagc ccccttggtg tggcaccgca tttattcacc cgtaatgaag gagcggttta
     1141 tgagttatga attagttaaa accctacaca tcctcagcat ggtgctgctg tttggcaccg
     1201 gccttggcag tgcgttttac aaatggatgg ccgatcgcag cggcgcggtg gcccatatag
     1261 ccatcaccaa tcaacatgtg gttctggcgg actggctgtt caccaccccg accgtgatct
     1321 tccaaccctt aagcggcctg tggatggtgc tgcatctggg cctgcccatc agcacgcctt
     1381 gggtcatgat cagccttggc ctgtacgccc tggcgggggc ctgttggctg ccggtggtgt
     1441 ggttgcaaat tcgtatgcgc agcctatcgg ctcaggctgc cgcagccgaa gaggcgctac
     1501 cggccgagta ttgggccctg gcgcgcatgt ggttttggtt gggggtaccg gcgtttatcg
     1561 ccatggtgtt ggtggtggcc cttatggttt ttaaacacat cccaggagtt gagttatgag
     1621 cagtttgatg caaagcgccc ttggcgcgca gttcgagcaa ttacccccag cacttaaggg
     1681 gcactatagg gcgggtaata ccctggagca ggggcacttg gatatcgaat acccccgctg
     1741 gatgcagccg tttttaacct tgttgcggct gctgggcgct ctggtgaacc gcagtggccc
     1801 caaggtgccc acccaggtgg aaaaattcga cagccccagc ggccagcgct ggcggcgcac
     1861 tattagtttc cccgatggca aggtggtgta tttcaatagc cgctgggtgc cggggcaggg
     1921 caatcgttta attgaatttg tgaacccaat ggtggggctg gagatggcgg tgcatgtggc
     1981 caatggcaag ctgtattacg aaggcgtgcg ctttgtggtg aagcttggca gcctgttaat
     2041 ccccattccc gagtggttgc tgctgggcca taccagcatt gaagagcagg ggctggacga
     2101 gcaccacttc gctatggact ttcgccttac ccacccgttg ttcgggcaga tattccgcta
     2161 cgcgggcacc tttgccatag agcctgagcc ggtggtgaac cggctagaat aagcgctttt
     2221 gttatgaagc gcgtttatgg cctacaccct tgcccccatc ggcattattc acagctgttt
     2281 tagccagaag ttcggcatac cccgccagcc gggcctggcc cctgccgcca ccggccagtt
     2341 agtcctgctg ccacctttta acaacccgga ggcagtggca ggccttgagg gggtgagcca
     2401 tctgtgggtg gagtttgtct ttcatgctgg ctacggcggc tggaagccac gggtgaaaat
     2461 gccgaggctc ggcggcaata aaaccctcgg cgtgtttgcc acccgctccc ccaaccggcc
     2521 caataaccta ggcctctccg tggtgagcct acttaaagtt gagctggagc cccaggtgcg
     2581 cttaaccctc ggcggggtgg atatgctcga tggcaccccg gtactggata tcaaacccta
     2641 tttgccctat gccgatgtaa ttgaaaatgc ccgcaacgac tgggccgcag agccgcccgc
     2701 attaattgcc gtgcagtttt ccccgcaggc tcaggcgcaa tttagcgagt taaacgcgcg
     2761 ctaccccaag ctggccgaac ttgccgccca ggtattaagc caagacccta ggccctccta
     2821 tcaggactac actagtgagc gcacctacgc gctgcggttg tgggatgtgg atattcactg
     2881 gcgctgcgtg gcggcttcta cgggctttat gattgaagta ctgttctgcc gcttggttgt
     2941 gcccgattaa aaccggcgca gtttaattat ttattcttaa aaagaagggg aagactatgt
     3001 ttaaaagttt ggcggcagat gtattgggcc taagtgatat tggcaaaatc ctggataaaa
     3061 aggattttga taaggccgat gtggatgact atgtatttgc agaggatggt gagcacattt
     3121 atgtggtgat taaatccaaa actgatgaat actgttttac caatttagcc atgattcact
     3181 tagatggctc ctcggcggtg agtaaaaagc gcctgctgca ccgctacccc tataaacacc
     3241 acccggtgag ccacgtgcgc attgaaacag cgggtacggt ggatttagac gtggaattaa
     3301 aatttaaact tgccgataaa gaatattcca ttgatataga taaaacccaa attgagcggg
     3361 tgaaaaatat ttacaaggcg cttacggtaa taggcgaaaa atgccgggaa atccaccgcc
     3421 aaatggaaac cctgcgccac acctgcgcgg cggtaaatgg catgtttgtg ttgcgggagt
     3481 tgcccgaaca agtagtgctg agcctgccgg atattattaa ccaaacggtt ttgcaggtag
     3541 aggcccacta taacgagcgc cgcaaagaaa ttcagaatta cgattttggt gaggtgtttg
     3601 ggcgctatat taagtggtag gtaactattt agcacacctt attgcccttt taagcgcggg
     3661 cggcaaatta acagcttgtt gaaatactac cttgctagct ctggcggcgt taaatttggc
     3721 g
//
LOCUS       DAOQGS010000416         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14076_length_3696_cov_4.544631, whole genome shotgun sequence.
ACCESSION   DAOQGS010000416 DAOQGS010000000
VERSION     DAOQGS010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14076_length_3696_cov_4.544631"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2291)
                     /locus_tag="PKZ52_13855"
     CDS             complement(<1..2291)
                     /locus_tag="PKZ52_13855"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG60904.1"
                     /translation="MWIVSIALRRPYTFVVAALLIVLAMIYVLGKMATDIFPEIKIPV
                     VAQLWEYKGLTAKEMSDRITSQAERELGQAVDNIEHTEAFSVAGLAIVKVFFQPGTDI
                     NAAMTQTTMSANAAWRSMPSSAQPGRVRRYSASELPVVQMGISSDTLSDIEIGDAAGT
                     VLRPIVSQKKGLTLTAPFGARSRQISVDIDANALLANNLTPAELVTAMGAQNLILPTG
                     TMKIDDSEFDIKLNGSVPNINEIGLLPIRSEQGRTLLVRDVANVHDGFSPTTTLARQN
                     GSRSVLNSVLAYGGVSTIDAVNFIKDSIPEMLRKMPDGVNIKLMFDQSVFVRSAINNV
                     LHEGLIAASLTATMILLFLGNWRSTCIIAVPIPLSIMVSLICLYLIGQTINLMTLGGL
                     ALAVGILVDDATVEIENIERQMALGKNPMQAILDGAEEIAMPALVSTLCICIVFVPMF
                     FLTGIAQYLFVPLAQAVIFAMLASYIISRTLIPTLVMFMMRNHHHSKEASNNPVARSF
                     AKLHTGFNRGFERLQQQFAALLTAIFRRRKQCAAGFLIFCIGSLGLYPLLEQDLFPTV
                     DSGQLRLHIRAPSGTRLEQMPYLVDQIESHIREVIPDAQEGDILDIIGGPYSTINTLN
                     GNSGTAESSDSEIMISFKRGALGADDFMRELRLQLPQAFPQVEFFFQPPDQISQTLNF
                     GIPAPIDVQFIGGKQTETIPLAQDVTNELRKIPGLVDVNVFQRLSKPTLALEMNRTQL
                     QQLGLSARNVAENMLIALSGSMQT"
     gene            complement(2732..3121)
                     /locus_tag="PKZ52_13860"
     CDS             complement(2732..3121)
                     /locus_tag="PKZ52_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNG60905.1"
                     /translation="MDEHFMAPSGPLTEEHIAKMVAVFYQRARAHPSLGRVFNTVVAD
                     WDHHLAVVQDFWSRALLATNRYSKHPYPAHVDLPIKREHFAQWLELFRPAALENLPQE
                     DAYTAIAKAEQIAESFRRGLFPFDPIN"
     gene            complement(3240..>3696)
                     /locus_tag="PKZ52_13865"
     CDS             complement(3240..>3696)
                     /locus_tag="PKZ52_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60906.1"
                     /translation="LMIGVIMFEHAELKGEKHRILQAADTYTAPEILESRETAAGRLQ
                     HTVVNALLVERYLPPEQKGDSWPHDATGTPWWTVVARPPHVGLVAAFNAAMGGSYLRK
                     LDPKHNVVAFAAQGEEVIMNKGEYCVVRASDKTELRQANLVALRASYYA"
BASE COUNT          935 a          919 c          941 g          901 t
ORIGIN      
        1 gtttgcatgc tgccgcttaa ggcaatcaac atattttcgg ccacattgcg cgcacttaaa
       61 cccagctgct gtaattgggt gcgattcatt tccaatgcca gggtgggttt acttaaacgc
      121 tgaaagacgt ttacatccac taaacctgga attttacgca gttcattcgt tacgtcctgt
      181 gccaatggaa tagtttccgt ttgtttgccg ccaataaact gcacgtcaat gggggcgggt
      241 ataccaaaat ttagggtctg gctaatttga tccggtggct gaaaaaagaa ctcaacctgg
      301 ggaaaagctt gaggtaattg caaacgcagc tcgcgcataa aatcatccgc acccagcgcg
      361 ccgcgcttga agctgatcat aatttctgaa tcggatgact cggcggtgcc ggaattaccg
      421 ttcagcgtat taatagtgct gtaagggccg ccgatgatat ccagaatgtc gccctcctgt
      481 gcatctggaa tcacttctcg tatatggctt tcgatttgat ccactaaata aggcatttgc
      541 tcaaggcgtg tgcctgaggg cgcgcgaata tgcaaccgca gctgaccgct atccacagtg
      601 gggaataaat cctgctccag cagtggataa agccctaagg agcctatgca aaatattaaa
      661 aaccccgccg cacattgttt gcggcggcgg aaaatagccg taagcaaggc ggcaaattgc
      721 tgttgcaatc gttcaaaacc tcgattaaac cctgtatgca acttggcaaa actgcgcgca
      781 accggattat tgctcgcctc tttgctgtga tggtgattgc gcatcataaa catcaccaga
      841 gtggggatta gtgtgcgcga gataatatac gaggcgagca tggcaaaaat caccgcttgc
      901 gccaggggga caaacaggta ttgggctata ccggttaaaa aaaacatcgg cacaaacaca
      961 atacaaatac acaaggtgga aaccaacgcc ggcatggcga tttcctcggc gccatccaaa
     1021 atagcttgca tggggttttt gcccagggcc atctgccgtt caatattttc aatttccacc
     1081 gtggcatcat ccaccaaaat acccacggct aaggcaagcc cgcccagggt cattaaatta
     1141 atcgtctggc caattaaata caggcagatt agtgacacca taatagatag cgggataggc
     1201 acggcaataa tgcaggtgct gcgccagttg cccagaaata ataaaatcat ggtggctgtg
     1261 aggctggcgg caatcagccc ttcgtgcagt acattattaa tcgccgagcg cacaaatacc
     1321 gattggtcga acatcaattt tatattcacc ccgtcgggca ttttgcgcag catttcgggg
     1381 atggaatcct taataaaatt gacggcatca attgttgata cgcccccata ggcgagtacg
     1441 gaatttaata cgctgcgaga gccattttgg cgggcgagtg tagtggtggg cgagaaccca
     1501 tcgtgtacat tggccacatc gcgcacgagt agggtacggc cctgttcact gcggatgggc
     1561 aacaggccta tttcattaat attgggcacc gagccgttta atttaatatc aaactcactg
     1621 tcatcaatct tcatggtgcc ggtgggcagt attaaatttt gtgcccccat ggccgttacc
     1681 aattctgctg gggttaaatt attagccagt agggcgttgg catctatatc gactgatatt
     1741 tgcctgctgc gcgcaccgaa gggcgctgtg agggtgaggc cctttttctg cgaaacaata
     1801 ggccgcaata cagttcccgc tgcgtcgcca atttcaatat ccgataaggt atcgctggaa
     1861 atacccattt gcaccaccgg caattcggac gccgaatagc ggcgcacgcg gccgggttgc
     1921 gcactgctgg gcatagagcg ccaggcggca ttagcgctca tggtggtttg ggtcatggcg
     1981 gcattaatat ctgtgccggg ctgaaaaaac actttaacaa tggccaggcc cgcaaccgaa
     2041 aaggcttcgg tatgttcaat attatccacc gcctgaccca attcccgttc ggcttgggag
     2101 gtaatgcggt ctgacatctc tttagcggtg aggcctttgt actcccacaa ttgcgccacc
     2161 acgggaattt ttatttccgg gaaaatatcc gtcgccattt tgccaagcac ataaatcatg
     2221 gcgagcacaa taagcagtgc cgccacaacg aaggtgtagg ggcggcgtaa ggcgatcgaa
     2281 acaatccaca tggtatacag aaaagctcag cagcagttgg ttagaaataa ccaacctata
     2341 aaaataggaa cctgccaaag caatatgcac accagctata gcttcagtgc acctattaac
     2401 ccggcggtca aatagatcgt cctgatctat ttgaccttcg cagcgcaaaa actggcgaaa
     2461 atacgtggat tttcttgttt ttgcgcagct cgcggcggtt tgcaccgccg agtgcggcat
     2521 ccttgccgcc attattaacc cggcattaaa tgccgccggg tcaataatag gccttattat
     2581 cgattgcgct ggatagggcg ttgggttgtg taatttccac gggaatacgc gctgttgaaa
     2641 tttgcccaga taatggggcc agggttgtta tttcaacagt attaccctgg tgagcacagg
     2701 cgcagtgggc gcgcttgcat gtcacattaa tttaattaat aggatcaaac ggaaataagc
     2761 cacggcggaa actctcggca atctgttccg cttttgcgat tgctgtgtac gcgtcctctt
     2821 ggggtaaatt ctctagggcc gccgggcgaa ataattctag ccactgggca aaatgttcgc
     2881 gttttatcgg taaatccaca tgggccgggt aggggtgctt gctgtagcgg ttagttgcta
     2941 aaagtgctcg cgaccaaaaa tcctgcacca ccgccaagtg gtgatcccaa tcggcaacca
     3001 cagtattaaa taccctacct agggatggat gggcgcgggc gcgttgataa aaaacggcca
     3061 ccatcttagc gatatgctct tcagttagcg ggccagatgg cgccataaaa tgctcatcca
     3121 aaaatacacc taccggtggg agttgccggg attggaaatt attgcgcgca atttgggtgt
     3181 gagcgcaaaa ataaatgccc gcccgaatgg gatattacat tcggacgggc cttgcgcttt
     3241 taggcgtaat acgaagcccg taaggccact aaattcgctt ggcgcagttc ggttttatca
     3301 ctggcgcgca ccacacaata ttcgccctta ttcataatta cttcttcgcc ctgggcggca
     3361 aaggccacta cattgtgttt cgggtccagc ttgcgtaaat agctgccgcc cattgcggca
     3421 ttaaatgccg ccaccaaacc cacgtggggt ggccgcgcca ccactgtcca ccagggcgtg
     3481 ccggtggcgt cgtggggcca gctatcgcct ttttgttccg gcggtaaata gcgctccacc
     3541 aataaggcat taaccaccgt gtgttgtaaa cgcccggcgg ccgtttcccg agattctaaa
     3601 atttccgggg cggtataggt gtcagcggct tgtaagatgc ggtgtttttc acctttgagt
     3661 tctgcgtgtt caaacataat tactccgatc attaat
//
LOCUS       DAOQGS010000417         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14134_length_3686_cov_5.506197, whole genome shotgun sequence.
ACCESSION   DAOQGS010000417 DAOQGS010000000
VERSION     DAOQGS010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_3686_cov_5.506197"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PKZ52_13870"
     CDS             <1..1536
                     /locus_tag="PKZ52_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNG60907.1"
                     /translation="PLRAARLHASLKKVRGNRKALVENIQAAIEARLAREDIIGDVIG
                     REKHLYSIYNKMRSKNKSFNEIMDVYAFRIIVEDINTCYRTLGAVHNLYKPMAGEFKD
                     YIAIPKINGYQSLHTVLIGMHGVPIEVQIRTKEMDELAGRGIAAHWLYKSVGGDEHND
                     PPVARVDRWISRILELQQHTGSSIEFVENVKMDLFREEVYVFTPKGEIIELPQGATPV
                     DFAYAVHTQVGNRCVACRINDRPAPLSQALQSGQKIKIITSESSQPNPNWLNFVVSAK
                     ARSAIRHYLKHQRHMESVELGKRMLSRALSNLGMQLEQLAEREFNHLLEANKVSCVEE
                     LYESIGMGKRVALSVAQCLKPDGENNGYTPAPLTIDSSDGLLINFARCCRPIPGDPIV
                     GHLSTGKGLVVHRESCKNVTDLRSKRENITEVNWSIKVAGEFLTDIRVEVESERGIIA
                     SLATRIAETRTSIEGINVSERDAQHSVITLTIGVRNRVHLADVMRRVRTMRSVSKVSR
                     AKS"
     gene            1590..1973
                     /locus_tag="PKZ52_13875"
     CDS             1590..1973
                     /locus_tag="PKZ52_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNG60908.1"
                     /translation="MTNKAIIFTDKAPAAIGTYSQAVKVHNTVYLSGQIPLVPATGAL
                     VEGDFAEQARQVFRNLKAVCQAADGDLKDIVKLNLFLTDLSNFPIVNTVMAEFFEAPY
                     PARAAIGVKELPKGAVVEADGIMVI"
     gene            complement(2151..2750)
                     /gene="ssb"
                     /locus_tag="PKZ52_13880"
     CDS             complement(2151..2750)
                     /gene="ssb"
                     /locus_tag="PKZ52_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNG60909.1"
                     /translation="MVARGVNKVILVGNVGQDPEVRYTAGGSAIANLSIATSEAWRDK
                     QSGQMQERTEWHRVVFWARLAEIVGEYVKKGSKIYVEGRLQTRKWSDPQGVERYTTEI
                     VASELQLLDGRGAGGPQAGGADDGYGGGYGQGGYEPAGNYGAAPAYAAQTPQPQAPAR
                     NPAPSYPPRQAPSRPAPANQAPPQPAPPAMDSFDDDIPF"
     gene            complement(2806..>3686)
                     /locus_tag="PKZ52_13885"
     CDS             complement(2806..>3686)
                     /locus_tag="PKZ52_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG60910.1"
                     /translation="SLDHARHQEQPRFHRRGCYILLRQVPNPPPTVRGKDAIPVPAMI
                     GQSLRNSELARLNVGIFSLHFILSALFVALPLAMQDAGIAATDHWQFYGPILALAFIA
                     MMPFMHLAERKRKVKPVLLAAIAGLVVGLVLLQLAGTAWLGWAGGLFVFFMSFCLLEA
                     TLPSLLSKQAPAGAKGTAMGLFSTCQFLGISAGGSLGGYWVQHHSTLTVFYYAAVLAS
                     LWFALALFMKKPVYLTSLSVALQQEPSVEALKQAIPGVQEAAWVQEQGLLHLKVKDEE
                     FDRQQLDQYLARLTAL"
BASE COUNT          910 a         1034 c          961 g          781 t
ORIGIN      
        1 cccctgcggg cagcacgttt acacgcctcc ttaaaaaaag tgcgcggcaa ccgcaaagcc
       61 ctggtggaga atattcaggc cgccattgaa gcgcgcctag cccgcgagga cattattggc
      121 gatgttatcg ggcgggagaa acacctctac agcatttaca acaaaatgcg cagcaaaaat
      181 aaatccttta acgaaattat ggacgtgtac gcgtttcgca ttattgtgga ggatattaat
      241 acctgttatc gcaccctggg cgcggtacac aatttgtata aacccatggc cggggaattt
      301 aaagactata tcgccattcc gaaaataaac ggctaccaga gtttacatac tgtattaatt
      361 ggtatgcacg gtgtgcccat cgaagtgcaa attcgcacca aagaaatgga cgaattagcc
      421 ggccgcggca tagccgccca ttggttgtat aaatccgtcg gtggcgatga gcacaacgac
      481 ccgccggtgg cgcgcgtgga tcgctggatt tcgcgtattt tagagctgca acaacacacc
      541 ggcagctcca ttgaatttgt tgaaaacgtc aaaatggatt tgtttcgcga ggaggtatat
      601 gtatttaccc ctaaaggcga aattatcgaa ttgccccagg gcgcaactcc agttgatttt
      661 gcctatgcag tgcacaccca agtgggcaac cgttgcgttg cctgccgcat taacgaccgc
      721 ccggcgcctt tgtcgcaagc gctacaaagc gggcaaaaaa ttaaaattat tacctcagaa
      781 tcctcccaac ccaatcctaa ctggctgaat tttgtggtga gcgccaaagc tcgctccgcc
      841 attcgccact atttaaaaca ccagcgccat atggaatcgg tggaattggg caaacgtatg
      901 ctcagccgcg cgctcagcaa tttaggcatg caactggaac aattggccga gcgggaattt
      961 aaccatttat tagaagccaa caaagttagc tgtgtggaag agctgtatga aagcatcggt
     1021 atgggcaaac gggtcgcgct ttctgttgcg cagtgtttaa aacccgacgg cgaaaataac
     1081 ggttataccc ctgcgcccct caccattgat tcctccgacg gcttgttaat taatttcgcc
     1141 cgctgttgcc gccccatccc cggcgacccg attgtcggcc atttgagtac cggcaagggt
     1201 ttggtagtgc atcgagaatc ctgtaaaaac gtcaccgact tacgcagcaa gcgcgaaaat
     1261 atcaccgagg tgaattggtc tatcaaagtg gccggagaat ttcttaccga tattcgcgtg
     1321 gaagtggaat ccgagcgcgg tattattgcc tccttggcca cccgcatcgc cgaaacgcgc
     1381 accagcattg aaggcattaa tgtcagcgag cgggatgcgc agcattcggt aattacgctg
     1441 accatcggcg tgcgcaaccg ggttcatttg gcggatgtta tgcgccgcgt gcgcaccatg
     1501 cgcagcgtca gcaaagtctc ccgcgccaaa agttaatttc tctttaaccc tcctgcggca
     1561 gctgccgccc tttgtcttgg aattttttta tgaccaataa agccattatt tttaccgaca
     1621 aagcccctgc ggccataggc acctactcac aggcggtaaa agtgcacaac accgtttacc
     1681 tatcagggca aattcccttg gtaccggcga ctggggctct ggttgaaggg gattttgcag
     1741 aacaggcgcg ccaagtattc agaaacttaa aggccgtctg ccaagccgcc gatggcgacc
     1801 tcaaagacat agttaaattg aatttatttc tcactgattt gagcaacttt cccatcgtca
     1861 atactgtgat ggccgagttt ttcgaagcgc cctaccccgc ccgcgcagcc ataggcgtta
     1921 aagaattacc caaaggcgcg gtggtggaag ctgatggcat tatggtgatt taagcgcacc
     1981 catttatgca ggtttactgt tacccacgca attacataaa gccccgcaca gtaaacacag
     2041 ctgaacttag gcaaaaataa aaaggccgcc ctagggcggc ctttttattt caactttgct
     2101 ctactcgatt gatcttcagt acatgactgg acgactaacc gagcagacaa ttaaaacgga
     2161 atatcatcat caaacgagtc catggccgga ggggctggct ggggcggcgc ttgattggct
     2221 ggagccgggc gactgggggc ttggcgcggc ggatagctgg gggccgggtt gcgagccgga
     2281 gcctggggct ggggtgtctg ggcagcgtag gcaggggcag cgccataatt gccggcgggt
     2341 tcataaccgc cctggccata gccaccgcca taaccatcat cggcaccacc ggcttgaggg
     2401 ccgccagcac cgcgaccatc cagcagttgc agctcggagg ccacaatctc cgtggtgtag
     2461 cgctcaaccc cctgggggtc gctccacttg cgggtttgca ggcggccttc cacataaatt
     2521 ttgctgcctt tttttacgta ttcgccgacg atttccgcca ggcgcgccca aaacaccacc
     2581 cggtgccatt cggtgcgctc ttgcatctgg ccggattgtt tatcccgcca ggcctcggag
     2641 gtggcgatac tgagattggc aatggccgaa ccgccagcgg tataacgcac ctctgggtcc
     2701 tggccaacat tgcccactaa aattactttg tttacgcctc gagccacgat gagcctcctc
     2761 ttcaataaaa aactaaacca tgatgcgccc tgcgggcggt agatgctaga gcgccgttaa
     2821 ccgcgccagg tactgatcca actgctggcg atcaaattct tcatccttca ccttcaaatg
     2881 caacagcccc tgctcctgca cccaggccgc ttcctgcaca ccaggaatcg cctgttttaa
     2941 agcctcaacg ctgggctcct gctgcagggc cacagacaaa ctggttaaat atacaggttt
     3001 tttcataaat aaggcgagcg caaaccacag gcttgccaac acggcggcgt aataaaatac
     3061 cgttagggtg ctgtggtgct gcacccaata gccgcctaag ctgccgccag cggagatacc
     3121 gagaaattga caggtggaaa acagccccat ggcggtgccc ttagcaccgg cgggcgcctg
     3181 tttactcagc agggagggca gcgttgcttc taggaggcaa aacgacataa aaaacacaaa
     3241 cagcccgccc gcccagccca gccaggcggt gcccgccagc tgcagcagca ctaaaccaac
     3301 caccaagccc gcaatggccg ccagcagcac aggcttaacc ttgcgcttgc gctcggccag
     3361 atgcataaac ggcatcatgg cgataaacgc cagcgccaaa atcggcccgt aaaattgcca
     3421 gtggtcagta gccgcgatac ccgcatcctg catagccagc ggcagagcca caaacagggc
     3481 ggacaaaata aaatgtaacg aaaaaatacc gacgttcaac cgcgccaatt cactgttgcg
     3541 cagggactga ccgatcatcg cgggcaccgg gatcgcatct ttgccccgca cagtgggggg
     3601 cgggttgggc acctggcgca ataaaatgta gcagccccgg cggtggaatc ggggctgttc
     3661 ctggtgccgc gcgtgatcga gtgacg
//
LOCUS       DAOQGS010000418         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14355_length_3651_cov_5.015851, whole genome shotgun sequence.
ACCESSION   DAOQGS010000418 DAOQGS010000000
VERSION     DAOQGS010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14355_length_3651_cov_5.015851"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKZ52_13890"
     CDS             complement(<1..620)
                     /locus_tag="PKZ52_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNG60911.1"
                     /translation="MRWIFFSLLAANLGIFIWWQTAKPPASPVAHKYITPAGVGSVVL
                     LSEADKQAGGPPSPVPPAPEAIPVPVTPNPEAAPAPADKVDNSHAQCVLVGPYENSDQ
                     VQQVLQKLQAKEINAHLFELELAVSGGFQVYLDGFENRAQAKKRLDELQAGGIDSFIV
                     PKGEFINTIALGSFDQEGAAKGQQEKLAAAGIAAKIRESKRPVMEL"
     gene            complement(631..1383)
                     /locus_tag="PKZ52_13895"
     CDS             complement(631..1383)
                     /locus_tag="PKZ52_13895"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNG60912.1"
                     /translation="MSRLEIDLGNSRAKWRLPALSLSGNLPSNALTAADELPPEWAGL
                     QVTSIWLGSVLAEADTESAIAKLQRFFACDVHRAYSSPAAPGLINGYAEPSRLGVDRW
                     LALLAARQHRGGDLVVVDAGTAITLDLVRADGTHLGGYILPGVARQAQSLWGGTGRVQ
                     VERAAMQPRFTPGQTTQECVAAAITAAIAGLCLLARQSLPEAAWCLTGGDADWLAAIC
                     GELHLSHAFYPDLVLDGLAWAQMQVYPAPQLN"
     gene            complement(1387..2355)
                     /locus_tag="PKZ52_13900"
     CDS             complement(1387..2355)
                     /locus_tag="PKZ52_13900"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNG60913.1"
                     /translation="MIERSQQLLSLLSDGRYHSGEALGSALGISRAAVWKLVQQLQAQ
                     QVPVVSRQGRGYCWDGATDLLSQAAITGALSEPVAVMLEVLWQTASTNSLLLEAARSG
                     SIHRRVILAEQQTGGRGRRGRTWVSPLAQNLYLSLGWHFEAGIAQVEGLSLAVGVVVV
                     QSLQALGIEGLSLKWPNDIWLHGRKLAGVLIEVGGDLSGQFHLVLGLGLNSHMPVEEA
                     SAQGWASLLEQRVLSRNLLAARLIDALSQLLEVFAARGFVYYQQEWNRLNGLADCAVL
                     IDQAGTLEEGVCIAAAPNGGLQVRTSTGLKTLLGGEISLRLQPNRV"
     gene            complement(2352..3485)
                     /gene="alr"
                     /locus_tag="PKZ52_13905"
     CDS             complement(2352..3485)
                     /gene="alr"
                     /locus_tag="PKZ52_13905"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNG60914.1"
                     /translation="MAAISLLSVFMSRSYPILNIDLGALASNYHLLQQRASGARVGAV
                     VKANAYGLGVEAVAACLYGRGCRDFFVSSTEEGVALRPLVPRAQIFVLQGVEPGDEGL
                     CQAFELTPVLISASMAKRYLSLGPATDFALKVNTGMNRLGLEPEQLLALVPELPVTGC
                     RLLMSHLACADEPAHSLNAEQLRQFQYLAEVARKALPQLQLSLANSAGIFLGPAYHFD
                     LLRPGAALYGLNPGNVAEGLLAPVVQLLLQVIQTRTLRAGDCVGYGATFRAGGETPVV
                     MVSGGYADGLMRALGQRGCAWFNGLRLPLIGRVSMDSLVFDVSGLPPAHRPVEGDRLE
                     FFGPNISVDAQAQVAGTIGYELFTRLGERCQRQYLNAAKGVGA"
BASE COUNT          767 a         1204 c         1050 g          630 t
ORIGIN      
        1 cacaactcca tcaccgggcg tttgctttcg cggattttgg ccgcgattcc agcggcggcg
       61 agcttttcct gttggccctt ggcggcgcct tcttggtcaa acgaacccag ggcgatggtg
      121 ttaataaact cgcccttggg cacgataaag ctgtcaatgc cgcccgcctg aagctcatct
      181 aagcgttttt tcgcctgggc gcgattttca aagccatcta ggtacacctg aaaaccaccg
      241 gagaccgcca gctccaattc aaacagatgg gcgttgattt ccttcgcctg caatttttgc
      301 agcacctgct gcacctgatc gctgttctca tagggcccca ccaatacaca ctgagcgtgg
      361 ctgttatcga ccttgtcagc aggcgccgga gccgcttctg gatttggggt aaccggcacc
      421 gggatcgcct cgggcgctgg tggcacaggg ctgggcgggc cgcccgcttg cttgtccgct
      481 tcgctgagca acaccaccga acccaccccg gcaggtgtta tatatttgtg ggccaccggg
      541 ctggcggggg gtttggcggt ttgccaccaa atgaagatgc ccaggttagc ggccagtaga
      601 gagaaaaaaa tccaacgcat aggggtttat tcaattaagt tgtggtgcgg ggtacacctg
      661 catctgcgcc caggccaggc cgtctaacac caggtcggga tagaacgcat gactgaggtg
      721 caattcaccg cagatggcgg caagccagtc ggcatcgccg ccggttaggc accaggccgc
      781 ttcaggtagc gactgccgcg ccaataggca taggcctgcg atggcggcgg tgatggcggc
      841 cgccacgcac tcctgtgtgg tctgcccagg tgtaaaacgc ggctgcatcg ccgcgcgctc
      901 aacctgtacg cgaccggtgc caccccacag gctctgcgcc tggcgggcca ctcccggcag
      961 gatatagccc ccaaggtgag tgccatcggc gcgcaccaaa tccagtgtga tagcggttcc
     1021 cgcatccacc accaccagat cgccgcccct gtgttgccgg gccgccaaaa gcgccaacca
     1081 gcggtctaca cccaagcggc tcggttcagc atagccatta attagcccgg gcgctgcagg
     1141 gctgctgtaa gcgcgatgca cgtcacaggc gaagaagcgc tgcagcttgg caatggcgga
     1201 ctctgtatcg gcctccgcca acaccgagcc gagccaaatg ctggttacct gcaggccagc
     1261 ccactctggc ggcaattcgt cggcggccgt taatgcgttg ctcggcaggt tgccactcag
     1321 gctcagagcg ggtaggcgcc atttcgcgcg gctgttgcca agatcgatct ctaaccggga
     1381 catggcttac accctgttcg gctgcaggcg caggctgatt tcgccgccga gcagggtttt
     1441 cagtcccgtg ctggtgcgca cctgcaggcc accattgggt gcggcggcga tacacacgcc
     1501 ctcctccaag gtgccggctt gatcaatcaa caccgcgcaa tcggccaagc cgtttaagcg
     1561 gttccactcc tgctggtaat agacgaagcc cctagcggca aacacctcca gcaattggct
     1621 aagggcgtcg atgagacgcg cagccagcag gttgcgactg agcacccgtt gctccaaaag
     1681 actcgcccac ccctgagcgc tcgcctcttc aacgggcata tggctgttga gccccagccc
     1741 cagcaccaaa tggaactgcc ccgataagtc tccgcccacc tcaattaata ccccggccag
     1801 tttgcggccg tgcagccaga tatcgttggg ccactttaat gataagccct caatccccag
     1861 cgcctgcagg ctctgcacca ccaccacccc cacggcaagg ctcaagcctt ccacctgggc
     1921 tatgcccgcc tcaaagtgcc aaccgaggga gaggtagaga ttttgcgcta aggggctgac
     1981 ccaggtgcgc ccgcgtcgtc cgcgcccgcc ggtttgctgt tcggccaaaa taacccggcg
     2041 atgaatgctg ccgctgcggg ctgcctctaa cagcaggctg ttggtggagg cggtctgcca
     2101 cagcacctca agcatcacgg cgacaggctc agacagggca ccggtgatgg cggcctgact
     2161 gagcaaatcc gtcgctccgt cccagcagta gccgcgcccc tggcgggaga ccaccggcac
     2221 ttgctgagcc tgcagctgtt gcaccagctt ccataccgcc gctcggctga tacccaaggc
     2281 gctgccgagg gcttcgccgg aatggtagcg gccgtcgctg agcagactca gcaactgctg
     2341 gctgcgctca atcaagcgcc caccccttta gccgcgttta aatactgccg ctgacagcgc
     2401 tcacccaagc gggtaaaaag ctcatagccg atggtgcccg ccacttgggc ctgggcatca
     2461 acactaatgt ttgggccgaa gaactccaaa cgatccccct ccacagggcg atgggcgggc
     2521 ggcaggccgc tgacatcaaa caccagggaa tccatggaca cgcgaccaat cagcggcagg
     2581 cgcaggccat taaaccaggc acagcccctc tgcccgaggg cgcgcatcag gccgtcggcg
     2641 taaccgccgg acaccatcac caccggggtt tcccccccgg cacgaaaggt tgccccatag
     2701 cccacgcagt cgcccgcccg cagggtgcgg gtttgaatga cttgcagcaa cagctgcacc
     2761 acgggggcaa gcagaccttc ggccacattg ccggggttga ggccgtatag cgctgcgccc
     2821 gggcgcagca gatcaaagtg ataggcgggg cctaaaaaaa ttccggcgga gttggcaagg
     2881 ctaagttgca actgcggcag cgccttgcgg gctacttcgg ccaggtattg aaactgccgc
     2941 aattgctccg cattgaggct gtgggcaggt tcatcggcgc aggctagatg gctcatcagc
     3001 aagcggcacc cagtgacggg caattcgggc accagcgcca acaattgctc cggctcaagc
     3061 cctaagcgat tcataccggt attgaccttt agggcaaagt cagtggcggg gccgaggctt
     3121 aaataccgct tcgccatgct ggccgagatg agcacgggcg tgagttcgaa cgcctggcac
     3181 aagccctcat ccccaggctc caccccttgc agtacaaaaa tttgcgcccg gggcaccaaa
     3241 ggccgcaatg ccaccccttc ctcggtggag gagacaaaaa aatcccggca gccccggcca
     3301 tacaggcacg cagccaccgc ctccaccccc aaaccatagg cattggcctt gaccaccgcg
     3361 ccgacacggg cgccagaggc gcgctgctgc aacaaatggt aattactggc cagggcgcca
     3421 agatcgatat tcaggatggg gtaactgcgc gacataaaaa cagacaggag ggaaatggcc
     3481 gccatctaac caaaccgcgc tgtaaaaggc tagtgctggc aagacctgcg gacctgcgat
     3541 tgtctccacc gccaacccag ataaacccgc aaagcggcca gataagtcgc cctcaaaaga
     3601 cccaaaacca aatcgatata aaaccgcgcc cggcccctcc cttgccccgc c
//
LOCUS       DAOQGS010000419         3644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14395_length_3644_cov_4.508498, whole genome shotgun sequence.
ACCESSION   DAOQGS010000419 DAOQGS010000000
VERSION     DAOQGS010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14395_length_3644_cov_4.508498"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..602)
                     /locus_tag="PKZ52_13910"
     CDS             complement(141..602)
                     /locus_tag="PKZ52_13910"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HNG60915.1"
                     /translation="MELILQPLSATKSIALIAHDNCKKHLIAWCQTHQSILKNHRLFA
                     TGTTGALIEAATELALTKLLSGPLGGDQQVGALIAEGKIDVLIFFWDPFEPLPHDPDV
                     KALLRVAAVWDVAVACTELTANFLIQSPLFNAPCERRVPDYGRYLNARVAE"
     gene            complement(806..1141)
                     /locus_tag="PKZ52_13915"
     CDS             complement(806..1141)
                     /locus_tag="PKZ52_13915"
                     /inference="COORDINATES: protein motif:HMM:NF016197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HNG60916.1"
                     /translation="MSHSFADLNRLLAALEMIMRRDGLWHHKSPSLEAMSSSMPFAVD
                     TMEFTDWLQFIFIPRMQTLIEQQLPLPAVCTIKPAAEVALMGRTGVEGLMEHLQQIDD
                     FFLQSAASY"
     gene            complement(1146..3254)
                     /gene="dinG"
                     /locus_tag="PKZ52_13920"
     CDS             complement(1146..3254)
                     /gene="dinG"
                     /locus_tag="PKZ52_13920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HNG60917.1"
                     /translation="MLDEDVKNIIQTSYRKYLQSLQLQARYGQKLMIAQIARTLGGVS
                     LSQDGERFGANHVCVVEAGTGTGKTVAYLLAAIPMAKARKKKVVISTATIALQEQIVY
                     KDLPDIAKHSGLNFTYVLAKGRGRYLCLSKLDRVLSADNQPLIPLYEELITEQDNQLF
                     QSMMSALSEGSWNGDKDAWPDELPQESWQKVTTDHRQCTGRKCSHVRNCAFFRARDAL
                     DDADVVVANHDLVLADLALGGGAILPAPKHTFYVFDEGHHLPDKALSHFACHTRVVST
                     VRWLGQTEGQCKHVLETIAGLVYVQTLALPLEAHLKNTRTKLEAVQPLLLPFIAQMDR
                     TASVVRYRFIQGEVPAELSVLAQQLHLLFAELARVLDKIVKELNRALEDEDSAVPKVD
                     VETAFTLFGSWCTRAESNSELFLSFANSTPNPKWPVARWITLISQGDVVDFELVSSPI
                     LASHTLNEHLWEKCAGAVVTSATLTALGNFERFKLRAGTPDETNYSVVPSPFDFASSG
                     VLHLPKQAIDATDANAHTLSIVQLLPQVLNANEASLVLFSSRKQMQEVQGLLPAAWQQ
                     KLLVQGDESKQRLLEKHRQRIDAGQGSVLFGLASFAEGLDLPGAYCQHVVIAKIPFAV
                     PDDPIEAAMAEWIEQQGGNAFMQIAVPDAAIKLVQACGRLLRTESDRGQVTLLDRRIV
                     SKRYGKAILNSLPPFKRDLQ"
     gene            complement(3244..3561)
                     /locus_tag="PKZ52_13925"
     CDS             complement(3244..3561)
                     /locus_tag="PKZ52_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60918.1"
                     /translation="MHLAFGAPLGLGFETVEQVADSLDDAIINNYVLHPSNCIAYEQL
                     TGQTPNLPVGADQRLFNSEDFVPERAFFAERLSRCEPRWQQKYIEAYANPVAAKLKLA
                     HVG"
BASE COUNT          947 a          912 c          886 g          899 t
ORIGIN      
        1 taggcgaatt tggcaaaaaa agcggagttt acacgcagta aatgagcatt ttttttgcca
       61 aattcaacga cgctagggcg agtgaggtaa tttttcagag gttccctaac gcaaagctgg
      121 atacgcccgg ggcaaataat ctactcggct acacgcgcat ttaaataacg cccataatct
      181 ggcactcgcc gctcgcaagg cgcgttaaat aaaggcgatt gaattaaaaa gttggcggtt
      241 aattccgtac aggccaccgc cacatcccac acggctgcca cccgcagcag cgcttttaca
      301 tctgggtcgt ggggcaaagg ctcgaaggga tcccaaaaga aaattaatac atcaattttt
      361 ccttcggcaa ttaaggcccc cacctgctgg tcgcccccta aggggccgct caatagttta
      421 gttaacgcaa gctccgtagc ggcttcaatt aatgcaccgg tagtgccggt ggcaaacagg
      481 cggtggtttt ttaatataga ttgatgggtt tggcaccagg cgattaagtg ttttttgcaa
      541 ttgtcatggg cgataagcgc tatggatttt gttgccgaca aaggttgcag aataagttcc
      601 atacccacct cgttaaaaat aaattaagtt gtgcgccact tattaacccg gcggcattta
      661 gtgccgggtt aataatggcg gcatggatgc cgccccggcg gtgtaaaccg ccgggagctg
      721 cgctaaaaca agaaaatccg cgtattttcg taggtttagc gcagcgcagg caaaatagat
      781 caggacgatc tattttgccg cctggttaat aggatgccgc agattgcaag aaaaaatcat
      841 caatctgctg caaatgctcc attaagccct caacacctgt gcgccccatt aaggctacct
      901 cagcagctgg tttgatggtg cagacagcgg gcaagggtaa ttgctgttcg atcagcgttt
      961 gcatacgggg gataaaaata aattgtagcc aatcggtaaa ttccatagtg tccaccgcaa
     1021 agggcatgct gctactcatc gcctcaaggc tcggcgactt gtggtgccat aggccatccc
     1081 ggcgcataat catttcaagg gcagcaagca gcctatttaa atcggcaaaa gagtgcgaca
     1141 tacaattatt gtaaatcccg tttaaaaggt ggcagagaat ttaaaatggc cttgccgtag
     1201 cgtttggaca caatgcgccg gtctaataag gttacctggc cccggtcgga ttccgtgcgc
     1261 aacaaacgcc cgcaggcctg cactaattta atagccgcat cgggtacagc aatttgcata
     1321 aacgcattgc cgccctgctg ctcaatccat tcggccatgg ccgcttcaat aggatcatcc
     1381 ggcaccgcaa agggtatttt ggcaattacc acatgctggc aataggcgcc gggtaaatcc
     1441 agcccttcgg cgaaactggc caaaccaaat aatacactgc cctgcccggc atcaattctc
     1501 tggcggtgtt tttccaataa acgctgttta ctttcatccc cttgcaccaa taatttttgc
     1561 tgccaagcgg cgggcaataa accctgcact tcttgcattt gtttgcggga ggaaaataat
     1621 accaggcttg cctcatttgc atttaatacc tgaggcaata actgcacaat ggagagagtg
     1681 tgtgcattgg catcggtggc gtctatggcc tgttttggca ggtgtaaaac accgctgctg
     1741 gcaaaatcaa aagggcttgg caccacggaa taattggttt catccggtgt gcctgcgcgc
     1801 aatttaaagc gctcaaaatt acccagggcg gtgagcgtgg ctgaagtaac cacagcgcca
     1861 gcacattttt cccacaggtg ttcattaagg gtatgagagg ccaaaatagg gctggaaacc
     1921 aattcaaaat ccaccacatc cccctggctt atcagggtaa tccagcgggc aactggccat
     1981 ttcgggttgg gtgtggagtt ggcaaaactt aaaaataatt cgctattact ttccgcgcga
     2041 gtacaccaac tgccaaacag ggtgaatgcc gtttccacat ccaccttagg caccgcgcta
     2101 tcttcatctt ccaaagcgcg gtttaattct ttaacaattt tatccagcac gcgggcaagc
     2161 tcggcaaata ataaatgtag ttgctgggct aacacactta attccgccgg cacctcgcct
     2221 tggataaacc tatagcgcac cacagacgcg gtgcgatcca tctgggctat aaacggtaat
     2281 aataatggct gcaccgcttc taattttgtg cgggtatttt ttaagtgcgc ttctaagggc
     2341 agcgcgaggg tttgtacata caccaggccc gcaatggttt ctaatacatg tttacactgg
     2401 ccttcggttt gccccagcca gcgcacggtg gagacgacac gcgtatggca ggcaaaatgg
     2461 ctgagggctt tatccggtaa atgatggcct tcatcaaaga cataaaaggt atgtttaggg
     2521 gcaggtaaaa tcgcgccgcc gccgagggct agatccgcta atactaaatc gtgattagcc
     2581 accaccacat cggcatcatc cagggcatcg cgggcgcgga aaaatgcaca attgcgcacg
     2641 tggctgcatt tgcggccggt gcactggcgg tgatccgtgg tgactttttg ccagctctct
     2701 tgcggtaatt catcgggcca ggcgtctttg tcgccattcc agctgccctc gcttaaggcg
     2761 gacatcatgg attgaaataa ttgattatcc tgctcggtaa ttaattcttc ataaagggga
     2821 attaacggct gattatccgc cgataaaacg cgatccaact tgcttaaaca cagataacgc
     2881 ccgcgcccct tggctaatac ataggtaaag tttaagcccg aatgtttggc gatatccggt
     2941 aaatccttgt aaacaatctg ctcctgcagg gcgatagtgg ccgtggaaat caccaccttt
     3001 tttttgcgcg ccttagccat aggaatggcc gctaataaat aggcaacggt tttacctgta
     3061 cctgtgcctg cttctactac acacacatgg ttggcgccga agcgttcacc atcttgggat
     3121 agggacacgc caccgagggt acgggcaatt tgcgcgatca ttaatttttg cccgtagcgc
     3181 gcctgcagct gcaagctttg taaatacttg cggtaactgg tttgaatgat gttttttaca
     3241 tcttcatcca acatgggcga gcttcaattt tgccgcgacc ggattggcat aggcttcaat
     3301 atatttttgt tgccagcgcg gttcacaccg gcttaagcgc tcggcaaaaa acgcccgctc
     3361 cggcacaaaa tcctcgctgt taaataagcg ttgatcagcg cccacaggta aattaggggt
     3421 ttgcccagtc aattgctcat aggcgatgca attggacggg tgcaatacat aattattaat
     3481 aatggcatcg tccaggctgt cagcgacttg ttctaccgtt tcaaacccca agcctaaagg
     3541 cgcaccaaaa gctaaatgca cagcgccttt aaaaccggta atacctaggg caatacataa
     3601 ttattaataa tggcatcgtc caggctgtca gcgacttgtt ctac
//
LOCUS       DAOQGS010000420         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14437_length_3638_cov_4.258164, whole genome shotgun sequence.
ACCESSION   DAOQGS010000420 DAOQGS010000000
VERSION     DAOQGS010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_3638_cov_4.258164"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKZ52_13930"
     CDS             <1..778
                     /locus_tag="PKZ52_13930"
                     /inference="COORDINATES: protein motif:HMM:NF019256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HNG60919.1"
                     /translation="GGVIASGAYMASNAAMNRTSPIFRSVHMRQDMLCQAIALPSNLN
                     DTAARDEAQKKVEALLASGNLTTAEFYNIQTTVPGSSCSTCHDAIINPLFAVDDFDHV
                     GLPRKQSGGQIVQKAFMGDAGEIGKDGVPIPMVNQGGRLYSANAVGVLGSYEANQDKR
                     NGGGLAFSGSKDLGQKMVAAGLKGLDTCLFEKTARFSLGHSLNPAFEESGLEKLLTAQ
                     QKSHMSCVTNAMEAAYTNSNGSARAAMKALGLSDVIRYRK"
     gene            870..2228
                     /locus_tag="PKZ52_13935"
     CDS             870..2228
                     /locus_tag="PKZ52_13935"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG60920.1"
                     /translation="MKKIIEKLKAAEQAEHHAQAQEAKVNYARYAREHEKTHERAQKQ
                     INKDRRGFLDMLHKAGIGASLLRVSSLAAGLMASRYALAAGGGKKVIYCYLDSGAATG
                     HWLPRSATSMNTVTKVYGPQGTDVASLCHFRQVNVALSGHSAAHQSLGALQSGVPTMD
                     ARIAPLLSAAAPYRAIYLGSDATVSGSLCSTIGPCLDDPAAAYTRYFNSALPAGSTDD
                     TYTKVFDSQKKALAQLRSKLSQDEKQRIDEHAEALAGLESRVAALMSGSGPDIETYRP
                     TLPAASTYLNRTVARGKVQADIMIAALQAGLTNVGVLQLGNHQGTWSGDGTVFKDTLH
                     SSAHNNPTDPTAFNEMIGHLSQVPAYIIKRLTDTTGADGQKLIDSTVFVQVTCMGNGM
                     THEPADAPFIVATRMPGFARGFSASAGGGTEDLNGAIPKGLGLADGTYAPMGKNSLGL
                     LT"
     gene            2272..3606
                     /locus_tag="PKZ52_13940"
     CDS             2272..3606
                     /locus_tag="PKZ52_13940"
                     /inference="COORDINATES: protein motif:HMM:NF019554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNG60921.1"
                     /translation="MNRIPPALADLLQCPVCASPLSALSCSHCQTEFFRFGDLLSFMP
                     AGRWQHKLWQHQLALNNQQAHMGMSAMQALLERCDLVPTTYQRLQECLQIAEQSLASI
                     EDLMAEVGLSPEFDEQFSHLSLGAISEYYHHILQDWAWGDEAINQRLAPILALDLPGN
                     PGTVLALGAGAGRLSWELHRRWGARLSLACDINPLLLAASHKLIVKQEPFSFYELNSF
                     PQINQARSGAYALTPPPGSAAERASWHALAADVWHMPIKPNSLDLVLTAWFIDVHGGD
                     NRTLIGLIERWLKPGGVWVNSGPLLYPKATPLECKYDREELLELMQIAGLSCEQERLD
                     EQGHLMSPINVRRQTEQVWTFAARKPLVAPVSPQDLPPAWLLFHHLAVPRFFHAPAES
                     HPLIDQILAQVDGERSIEAIAALTASQFPEGLDPRATIVEILGELFKPKNRS"
BASE COUNT          778 a         1170 c          995 g          695 t
ORIGIN      
        1 tggtggtgtg attgcctccg gtgcctacat ggcctccaac gccgctatga accgcacctc
       61 gccgatcttc cgctccgtgc acatgcgcca ggatatgctc tgccaggcca ttgccctgcc
      121 cagcaactta aacgataccg ccgcccggga tgaagcgcag aagaaagtag aagcgctgtt
      181 ggcatcgggt aatttaacca ccgccgagtt ctacaacatt caaaccacgg tgcccggctc
      241 ctcctgctcc acctgccacg acgccatcat caacccgctg tttgccgtag atgattttga
      301 ccatgtgggc ctgccgcgca aacagtctgg cggccagata gtgcagaagg cctttatggg
      361 ggatgccggt gaaattggta aggatggcgt gcccatcccc atggttaacc agggtggccg
      421 cctctactcc gccaatgcgg taggtgtgct cggctcctat gaggccaacc aggataaacg
      481 caacggcggc ggcttagcct ttagcggctc taaagacctg ggccaaaaaa tggtggctgc
      541 gggcttgaag ggattggata cctgtttatt tgagaagact gcgcgcttct ccttgggcca
      601 ctccctcaac ccagcctttg aagagagcgg gcttgagaag ctgctcaccg cccagcaaaa
      661 atcccatatg tcttgtgtaa ccaatgccat ggaagcggcc tacaccaata gcaacggcag
      721 tgcgcgagcc gccatgaagg cactgggttt atccgatgtc attcgctacc gcaaataacg
      781 ctcgctagcc ctacgcgccg ggttcgcccg gcgctgtgta catcgagaag ccgagacaac
      841 cagaaaagac gaccagaaaa ggttacttta tgaaaaagat catcgaaaaa ctcaaggccg
      901 cagaacaggc cgagcaccat gcccaggccc aagaagccaa agttaattac gcccgctatg
      961 cccgtgaaca cgaaaaaacc cacgagcgcg cccagaagca aatcaataaa gaccgccgtg
     1021 gctttttgga tatgctgcac aaagccggca taggcgcgtc tttgttacgc gtctctagcc
     1081 tcgcggcggg ccttatggcc tcccgctacg ccctggctgc aggcggtggt aaaaaggtta
     1141 tttactgcta tttagatagc ggcgctgcca ccggccactg gctgccgcgc agcgcaactt
     1201 ctatgaatac cgtgacaaaa gtctatggcc cccagggcac tgatgtggcg agcctgtgtc
     1261 actttcgcca ggtgaacgtg gccctctccg gtcactccgc cgcccaccaa tctctggggg
     1321 cgttgcaatc gggtgtgccc accatggatg cgcgtatcgc gcctttactc agcgccgccg
     1381 ccccctaccg ggccatttac ctaggcagcg acgccaccgt gagcggctcc ttgtgcagca
     1441 ccatcggccc ctgtttggat gatccggcgg cggcttacac ccggtatttc aactcggccc
     1501 tgcccgccgg tagcaccgac gacacctaca ccaaggtgtt tgattctcag aaaaaagccc
     1561 tggcgcaatt gcgcagcaaa ttaagccagg acgaaaaaca gcgtatcgac gaacacgccg
     1621 aggccctagc cggtttagaa agccgtgtgg cggctttgat gtcgggctcc ggccccgata
     1681 tcgaaaccta ccggcccacg ctgcccgctg cctccaccta tttgaaccgc accgtggcgc
     1741 ggggcaaggt gcaggcggat attatgatcg ccgccctgca ggccgggctt accaacgtgg
     1801 gcgtgctgca actgggcaac caccagggca cctggtctgg ggatggcacc gtgtttaaag
     1861 acacgctgca cagctccgcc cacaacaatc ccaccgaccc caccgcgttt aatgagatga
     1921 tcggccacct aagccaggtg cctgcctaca tcatcaaacg cctcaccgac accaccggcg
     1981 ccgacggcca aaagctcatc gacagcacgg tgtttgtgca ggtgacctgc atgggtaacg
     2041 gtatgaccca cgaaccggcc gatgcgccct ttatcgtggc cacccgtatg ccgggcttcg
     2101 cccgcggctt ctcggcctcc gccggtggcg gcaccgagga tctcaacggc gccatcccca
     2161 aaggtttggg cctcgccgac ggcacctatg cgcccatggg caaaaactca ctgggtttat
     2221 taacctgatt ctgacctgag cggccctagg gccgcttttt ttaagctcgt tatgaatcga
     2281 atcccgcctg ctcttgccga cctgttgcaa tgcccggtct gcgcctcgcc cctcagcgca
     2341 ctcagttgca gccactgcca aacggagttt tttcgctttg gcgacctgct cagttttatg
     2401 cccgcaggcc gctggcaaca taaactctgg cagcatcaat tagccctcaa taaccaacaa
     2461 gcccacatgg ggatgagcgc catgcaagcc ctgctggagc gctgcgacct ggtgcccact
     2521 acttatcaac gattgcagga atgcctgcag attgcagagc agagcctggc cagtatcgaa
     2581 gacctgatgg ccgaggtagg cctaagcccc gagtttgacg aacaattcag ccacctcagc
     2641 ctcggcgcca tcagtgagta ttaccaccac atactgcagg attgggcctg gggcgatgag
     2701 gccatcaacc agcgcctcgc gccgattttg gcccttgatc tgccgggcaa cccaggcact
     2761 gtgctggccc tgggggccgg ggcgggcagg ctcagttggg agttgcaccg ccgctggggt
     2821 gcgcgcctga gcctcgcctg cgacatcaac ccactgttac tagcagccag ccacaagctg
     2881 attgttaaac aggagccctt cagcttttac gagctcaata gcttccccca aatcaaccaa
     2941 gcccgcagtg gcgcctacgc cttaacgccc ccacccggca gcgcggctga gcgcgccagc
     3001 tggcacgccc tcgccgccga tgtgtggcac atgcccatca agcccaacag cttggatctg
     3061 gtgctcacag cctggtttat cgatgtgcac ggcggcgata accgcaccct gatcggctta
     3121 attgagcgct ggttaaagcc cggcggcgtc tgggttaact caggccccct gctgtacccc
     3181 aaagccaccc cgctagagtg taagtacgac cgcgaggaac tgctggagct gatgcagatt
     3241 gcaggcttga gctgtgagca ggagcggctg gatgaacagg ggcatttgat gtcgcccatc
     3301 aatgtgcgca ggcaaaccga gcaggtgtgg acctttgccg cccgcaagcc actggttgcc
     3361 ccagtaagcc cgcaagacct gccgcccgcc tggctgctct ttcaccatct agccgtgccg
     3421 aggtttttcc acgctccagc cgagagccac ccgttgatcg accagatcct cgcccaggtt
     3481 gacggcgagc gctccataga ggccatagcc gccctcaccg cttcgcagtt cccagagggc
     3541 ttagacccca gggcaacaat tgtggaaatt ctcggggaat tatttaaacc taaaaaccgc
     3601 agttgaactc gtagcgaggg tgcctaagac gctgaata
//
LOCUS       DAOQGS010000421         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14452_length_3635_cov_4.903911, whole genome shotgun sequence.
ACCESSION   DAOQGS010000421 DAOQGS010000000
VERSION     DAOQGS010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14452_length_3635_cov_4.903911"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..423
                     /locus_tag="PKZ52_13945"
     CDS             25..423
                     /locus_tag="PKZ52_13945"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HNG60922.1"
                     /translation="MAITYTKAAQVMNEQIETITSHQIISLLMDGALERIDQGILALH
                     AQQLEDHLVLKEKLIAIINGLRNCLDFERGGDIALNLNDLYMYMAERLEDTEGDAHLL
                     ALTEVRHLLLEVKAGWDNMPKDIINQAVAA"
     gene            complement(490..1149)
                     /locus_tag="PKZ52_13950"
     CDS             complement(490..1149)
                     /locus_tag="PKZ52_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60923.1"
                     /translation="MNKLVTSASLIALCALASVAQAATIEINFDGVNSGSSANSAAPS
                     GIVFYQAHYVNDVDAYGDEISNTQKWQIDSQSTADYPVTVDNPASFGYGAAPSGTNAL
                     NALWQPVLMRFESALDLTGFSTVLDNSTYGDILPAAVYFLDSNKKILSQIAVDQTVPG
                     AVATLTTPVNGVREVLFSSGAFYDTVQISSVPLPPSAALFLSGLALLGTIKYRKKRTQ
                     E"
     gene            complement(1212..3176)
                     /locus_tag="PKZ52_13955"
     CDS             complement(1212..3176)
                     /locus_tag="PKZ52_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNG60924.1"
                     /translation="MNYLLQRSTLLSLAISACFSISAEAAPVVSRLTPPSQLFATQGA
                     ESGPIIARFIPGQRFDLQATVSPDAGKTISDVQFLVDGNPVPGSITLTGGAALNSGLP
                     LDTKVASLRAYTNTLVGVHTLSVKVTQSDNQQTTALGNFEIVGINANAGRKAKNIIIM
                     VGDGMGASHRTAARIMLNGYAQGKTKERLAMDSFPNTAMIMTASLNSIITDSAPGMSN
                     YVTGNKAQNNQEGVWPDDTKDAFDNPRFEYVSEFLARTQGKKLGIVTTADVFDATPAA
                     NAVHTSNRGAGTGIVDQFFDDANKTGLTVLMGGGRKWFLPNGTPGSARAASSDYSLPA
                     DIISAWGVSAGAVDPARNLISDFQNSGWTYASDLTSLNAAGNPNKLLGLFAYSNMNVA
                     LDKIAGRRGNGAVVNDFGFPNQPMLDEMTQKALDVLDANSPNGFVLMVEAASIDKQAH
                     NMDSDRFIVEAIEFDYAIKKAKDYAETHPDTLVLVTADHECAGVAIIGASTKTDADLA
                     ASANTVAGKRDAVVGVYDAAGFPKYTKAADGYPVSTDPDYKMLIGYASNVDRYEDWRT
                     NAQPLRDSQQPFNNTAPLNTYPTDPLSRDAATGFLITGQIPLSSAVAAHTGGDIPLSA
                     LGRGANLFGGVMDNTDVFFSIMQAAIGGAK"
     gene            complement(3225..>3635)
                     /locus_tag="PKZ52_13960"
     CDS             complement(3225..>3635)
                     /locus_tag="PKZ52_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60925.1"
                     /translation="AFKDFFKLPAGPQGLEPTEKLRALNNQRVRIRGFMAHEDDPTAG
                     VFLLTSQPANVAEKADGMADDLPAATLTVYMPPEDKSKILTYRPGLWVLTGTLQLGNQ
                     EDSNGRISYARLIMDKRDRSIAPTLPSKPSTAAR"
BASE COUNT          899 a          847 c          941 g          948 t
ORIGIN      
        1 ggatattttt tgaggatggt tattatggca attacgtaca ccaaagcggc gcaggttatg
       61 aacgagcaga tcgaaacaat tacctcccac caaattattt ccctgctgat ggacggcgcc
      121 cttgagcgca ttgaccaagg gattttagcc ctgcacgccc agcagttgga ggatcacctg
      181 gtgttaaaag aaaaactcat cgccattatt aatggcctgc gcaattgttt ggattttgag
      241 cgcggcggcg atatagcgct gaatttaaat gacttgtata tgtatatggc ggagcgcttg
      301 gaggataccg aaggggatgc ccatttattg gcactcactg aggtgcggca tttattgtta
      361 gaggtaaaag ccggttggga taatatgcct aaagacatta ttaatcaggc ggtggcggct
      421 taattcacgg taataaccag gcaattgcta aaaaaaagcc gccgagtaac cggcggcttt
      481 tttaatggtt tattcctgtg tacgtttttt gcgatattta attgtgccca gtagtgcaag
      541 gccggataaa aataatgcgg cactaggtgg cagaggtacg ctggatatct gcaccgtgtc
      601 gtaaaacgct ccagatgaaa agagcacttc gcgcacgcca ttgactggag tagttaatgt
      661 ggccactgcg cctggcaccg tttggtcaac ggcaatttgg cttaaaatct ttttattgct
      721 gtccagaaaa tacacggcag cagggagaat atccccatag gtgctgttgt ccaatacggt
      781 tgaaaagccc gttaaatcaa gggcgctttc aaagcgcatg agcacaggtt gccacagggc
      841 atttagcgca ttggtgccgg aaggagcggc gccataacca aagcttgccg gattatccac
      901 cgttactgga tagtccgcag tgctttggct atcgatttgc cacttctggg tgttagagat
      961 ctcatcgcca taagcgtcca catcattcac gtaatgtgct tggtaaaaaa caatacccga
     1021 tggagccgcc gaattggcac tgctaccgga atttacacca tcgaaattaa tttcgatggt
     1081 ggcggcctgg gcgacactgg ctagtgcaca aagggctatt aggctggccg aggtgactaa
     1141 tttattcata acttcacatc ctcttaaata ggtgcacata atgtgcacca ctgattattt
     1201 ggcatagcgc tttatttggc tccgccaatt gccgcttgca tgatggagaa gaacacgtcg
     1261 gtgttatcca tcacgccgcc aaataaattt gccccgcgtc caagagcgga gagcggaata
     1321 tcgccgccgg tgtgagccgc tactgccgaa cttaacggaa tttgcccggt aattaaaaag
     1381 ccggtggcag cgtcccgact gagcgggtcg gtaggatagg tatttaatgg cgccgtatta
     1441 ttaaagggct gctgagaatc gcgcagaggt tgtgcgttgg tgcgccaatc ttcataacga
     1501 tctacgttag aggcataacc aattaacatt ttgtaatcgg ggtcggtgct gacgggataa
     1561 ccatcggcgg ccttggtgta tttaggaaat ccagctgcat cgtacacgcc cactaccgca
     1621 tcgcgtttgc ccgcaactgt attagcgcta gctgcaagat cggcgtcggt ttttgttgaa
     1681 gcgccgatga tcgcaacccc ggcacattca tggtcagcgg taactaagac caaggtatcc
     1741 gggtgagttt ccgcataatc tttggctttt ttgatggcgt aatcaaattc aatggcttca
     1801 acaataaagc gatcgctgtc catgttgtgg gcttgtttgt cgatggaggc agcttccacc
     1861 atcaatacaa atccgttcgg gctattcgca tccagcacat ccaatgcttt ttgcgtcatt
     1921 tcatccaaca taggttgatt gggaaatcca aaatcattca ccacggcgcc atttccgcga
     1981 cggccagcaa ttttatcgag cgccacgttc atgttggagt aggcaaataa acctaatagt
     2041 ttattagggt tgcccgcagc attgagtgag gtgaggtcgg atgcataggt ccaaccgcta
     2101 ttttgaaaat cggagattaa attgcgcgca gggtccactg ctcctgcgga tacaccccag
     2161 gcgctgataa tgtcggcggg caagctgtaa tcgctactgg cggcgcgagc tgaaccaggc
     2221 gttccattgg gtaaaaacca tttgcggcca ccacccatca atacggttaa accggtttta
     2281 ttggcatcat caaaaaactg atccacaatt ccggtgccag caccgcgatt agacgtgtgc
     2341 accgcgttgg ccgcaggggt tgcatcaaac acgtcagcgg tggtgactat gccgagtttt
     2401 ttgccctggg tgcgcgctag aaattctgat acatattcaa aacgcgggtt atcaaaagcg
     2461 tctttggtat catctggcca aacaccttcc tgattatttt gcgctttatt tccggtaaca
     2521 taattcgaca tgcccggcgc agaatcagta ataattgaat ttaaagaggc ggtcataatc
     2581 atggcggtat tgggaaagct atccatggct aaacgctctt tagtttttcc ttgtgcatag
     2641 ccatttaaca tgatgcgtgc ggctgtgcgg tggctggcac ccatgccatc gcccaccata
     2701 ataataatgt tttttgcctt gcggcctgcg ttggcgttaa tacccacgat ttcaaaatta
     2761 cccagggccg tagtctgctg attatcactt tgggtaactt taacgctcag ggtatgcaca
     2821 cccactaagg tattggtata ggcgcgcagg ctggccactt tagtgtccag cggtaagcct
     2881 gaatttaacg ctgcaccacc ggttaaggta atggagccag gcactggatt gccgtctact
     2941 aaaaattgca cgtctgaaat agttttgccg gcatcagggc ttactgtggc ttgtaaatca
     3001 aagcgttgac cgggaataaa gcgagcgata attgggccgc tttcagctcc ttgcgtagca
     3061 aataattggc ttggaggcgt cagtcgagat accactggag ctgcttcggc gctaattgaa
     3121 aagcatgctg aaattgccag gctcagcaga gttgatcgtt gcaacaaata attcatggga
     3181 tttcctctca attaaatcac ggattttttg gcgaagcaca taccctagcg ggcagcagtg
     3241 gagggtttgg acggcagcgt tggcgctatg gagcgatcgc gtttatccat aattaatcgt
     3301 gcatatgaaa tgcgtccgtt actgtcttcc tgattgccca gttgcagtgt ccctgttaat
     3361 acccaaaggc ccgggcgata agtgagtatt ttgcttttgt cctcaggggg catatagacc
     3421 gtaagtgttg cagcgggtaa gtcatccgcc atgccgtcgg ctttctcggc cacattggcc
     3481 ggttgggatg taagtaaaaa cacccctgcg gtggggtcat cctcgtgggc cataaagccg
     3541 cgaatacgca cccgttgatt attgagggcg cgcaattttt ccgttggctc caatccttgg
     3601 gggcccgctg gcaatttaaa aaaatcctta aaggc
//
LOCUS       DAOQGS010000422         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14478_length_3631_cov_4.536633, whole genome shotgun sequence.
ACCESSION   DAOQGS010000422 DAOQGS010000000
VERSION     DAOQGS010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_3631_cov_4.536633"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..906)
                     /locus_tag="PKZ52_13965"
     CDS             complement(136..906)
                     /locus_tag="PKZ52_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNG60926.1"
                     /translation="MKLKLLSALVVSVCTAQAQAALVAGDLMFTSFNADEDGFSLVTF
                     VDLAPNTAVFFSDNEWNGAAIGAGGAFNSGESYSRWNSGGSLINAGSVIRFSAVDSTN
                     LASSIGSYSRVSVSGNTNYGIANSNETLYAYLGSAATAPSLFLSAITNGNFAADGSIA
                     NTGLVQGTSAIDLNGTATTTPDYGVYSGVRSGLASFAAYKVLVGDAANWTVDTTNGNY
                     ASTVPDVTAFSVSQVPLPAGVWLLGSALVGVAGLRRRV"
     gene            complement(1421..2071)
                     /locus_tag="PKZ52_13970"
     CDS             complement(1421..2071)
                     /locus_tag="PKZ52_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNG60927.1"
                     /translation="MIKTFSALVLGAGLLMGASAQAAAVGGFSNTLVLENFDDLGFVS
                     SGPITLAGGQVIATSNVLATYDSLPLDLGSNGTWGLDGFVAIGDYYNYTTSPSVTDSL
                     TFISFAALSSLGAELSVYKDAFSSGSLLLEALGVNGAVLESAQVSLDFNDPAAYNRAA
                     VFGFVRAANDIHGLRITGDGFVVDNLMTSAVPVPASLPLFLSGMGLLYALRRRKKA"
     gene            complement(2177..>3631)
                     /locus_tag="PKZ52_13975"
     CDS             complement(2177..>3631)
                     /locus_tag="PKZ52_13975"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNG60928.1"
                     /translation="GASYNWFLTDPRFNARTNPLEPHLYGWVVEIDPNNPASVPVKRT
                     AMGRFKHESAQVAISRDSNNNLRVAYYMGDDERNEYVYKFVAAGNFNPTNRAANRDLL
                     DRGTLYVARFTSNATAVAKTYRGEWVALLPDTLSVIANGAGGFYKLRELSQFGAPADG
                     VANLDDAQVQALILIKTRQAADAVGATMMDRPEWAALRTYGNGWGYSTTTGAGLGYNA
                     QYPLELFLTLTNNNRRGGGGSLSVNSPNGTTAAGSARPPVDLANPRPDNDFGHIIRWR
                     EDGNTVTATSFEWDIFVLCGDTATAKTLASNYQNANLVDGVAGTGPFYQGNINDTPNG
                     SANLGAPDGLWFDQFGRLWIQTDQQGDGGGDFNNLGANSMSCADPNTGEIRRFLTSPS
                     NCEVTGVMNTPDGKTLFVGIQHPGEDAPVTNPLQNSTWPQSQFNRNSAGEPLALVADA
                     NNPGQKVAAPVGAPIRPRSSILVITRTDGGVIGA"
BASE COUNT          815 a         1014 c         1062 g          740 t
ORIGIN      
        1 aatacacatc gtttcagacc gtcaaaaaga gggacctttt tgacgagcct acagggatgt
       61 atttacggcg tgtctgaaat gatgtgtatt cattgctggt gcctaggtag taataatttt
      121 aaatgcagcg tttaattaaa cccggcggcg cagtcctgcc acacccacca aggccgaacc
      181 caataaccaa acacccgctg gcagcggcac ttggctgacg ctaaaggccg tcacatcagg
      241 aactgtggat gcatagttac cgttggtggt gtcaaccgtc caattagccg catcgcccac
      301 caggacttta taggcggcaa aactggctaa accacttcgc acaccgctgt aaacgccata
      361 atcaggcgtg gtagtggccg tgccattaag gtcgatggcg gaagtaccct gcaccaagcc
      421 ggtattggca atcgagccgt cagcagcaaa attaccattg gtaatagcgc ttaaaaatag
      481 gctcggcgcc gttgcggcac tgcctaaata ggcgtatagc gtctcattgg aattggcgat
      541 accgtaattg gtattaccgc tcaccgacac ccggctatag gagcctatgg agctggctaa
      601 attagtggaa tccacggcgc taaagcgaat aacactgcct gcattaatca ggctgccacc
      661 ggaattccag cgtgaataac tctcgccaga attaaatgca ccaccggctc ctatggcggc
      721 accattccat tcgttatcac taaaaaatac cgccgtattg ggggcaaggt ccacaaacgt
      781 caccagagaa aaaccgtctt cgtcggcatt aaacgaggta aacattaaat cgcctgccac
      841 caacgccgcc tgagcttggg cggtgcatac cgatacaacc aaggccgata ataattttaa
      901 tttcataaca gcaccccttt ggaatttgag attccactgt gcaagcgggc aatgactaaa
      961 cgatgaaatt agtgtgaatt cttggtgacg tttctgttgc ataatcgccg cgagcgctag
     1021 cgcgggaata tcttttctca acaacagccg actacgaata gataacacag tggcactagc
     1081 tggcctcacc ctaagcaatg tcgctcagtt gcgagatagc agcctttttt cgccatgggc
     1141 agtgcctcaa attcccctcc tctggagggg tggcaggctt gcctgccggg gtggttgcgg
     1201 tggcggatcc cagtaatgga cgaggaatat ttcctatttt ttacctacag gagcagagca
     1261 ggccgcgaac acaaaacagc gcagcggcct taccacccca ccaccccacc cctgcggggc
     1321 acccctccaa aggaggggaa ttttaggccc tgcccatggc gagaatgtcc ggcaaagccc
     1381 aactcaactg agttaggcgc ccggctgcca ccgacaaaac ctaggctttt ttgcggcgac
     1441 gcaaggcgta caacaagccc atgccggaga ggaacaaggg cagtgacgca ggtacaggta
     1501 cggcggaggt catcaggtta tccaccacaa aaccatcgcc ggtgatacgc aggccgtgga
     1561 tgtcgttggc ggcgcggaca aagccaaaga ctgcggcgcg gttgtaggcg gcggggtcgt
     1621 taaagtccag gctgacctgg gccgattcca acacggcgcc gttcaccccc agggcttcca
     1681 gcaacaaact gccggagcta aaggcgtcct tgtacacact caattcagcg ccgaggctgc
     1741 tcaaggcggc gaaggagata aaggtcaggc tgtcggtgac gctggggctt gtggtgtagt
     1801 tgtagtagtc gcctatggcc acaaaaccgt ccaggcccca ggtgccgttg ctgcctaaat
     1861 ccagcggcag ggaatcataa gtggccaaca cgttggaggt ggcgatcacc tggccgccgg
     1921 ccagggtgat ggggcctgag gagacaaacc ccaggtcatc aaaattctct aacaccaggg
     1981 tattggagaa accacccacc gccgccgctt gggcggaggc gcccattagt aagcccgcgc
     2041 caagcactag ggctgagaat gtcttaatca ttgcagtact cctaaataaa acgtgtaggg
     2101 tcagatcgaa caggggtgcg gtacgcagcc aggcttttaa cctaaaaacc tgacggccat
     2161 tgccttaagc tttgccctag gcaccgatca cgccgccgtc ggtacgggta attaccaaga
     2221 tcgacgagcg cgggcggatg ggggcaccca ctggcgccgc cactttttgg ccggggttat
     2281 tggcatcggc caccagcgcc aggggctcac cggcgctgtt gcggttgaac tgcgactggg
     2341 gccaggtgct gttttgcagg gggttggtaa ccggcgcgtc ctcgcctggg tgttgaatgc
     2401 ccacaaacag ggttttgcca tccggagtat tcatcacacc cgtcacctcg cagttggagg
     2461 ggctggttaa aaagcggcga atctcgcctg tgttcgggtc cgcacaggac atggagttgg
     2521 cgcctaggtt gttgaagtcg ccgccgccat cgccctgctg gtcggtctgt atccacaggc
     2581 ggccaaactg gtcgaaccag aggccatccg gcgctcccag gttggcagag ccgttgggtg
     2641 tatcgttgat attgccctga tagaaaggcc cagtaccggc cacaccgtcc accaaattgg
     2701 cgttctggta gttgctggcg agggttttag ccgtagccgt atcgccgcag aggacaaaaa
     2761 tatcccactc aaaactagtt gccgtcaccg tgttgccgtc ttcacgccag cggatgatgt
     2821 ggccgaagtc gttatccggg cgcgggttgg ccaggtccac cggcgggcgg gcggagcctg
     2881 cagcggtggt gccgttgggg ctgttcacag acaagctgcc accgccaccc cggcggttgt
     2941 tgttggtgag ggtgaggaac agctccaggg gatattgggc gttgtagcct aggcctgcgc
     3001 cggtggtggt ggagtaaccc caaccattgc cataggtgcg cagtgccgcc cactcggggc
     3061 gatccatcat ggtggcgccc acggcatcgg cggcctggcg ggttttaatc aaaattagcg
     3121 cctgcacctg ggcgtcatcc aggttggcca ctccgtcggc tggggcgccg aactggctca
     3181 gctcgcgcag tttgtaaaaa ccgcccgcgc cattggcgat taccgatagt gtatccggca
     3241 gcagggccac ccactcgccg cggtaggttt tcgccactgc cgtggcgttg ctggtaaagc
     3301 gcgccacata cagggtgccg cgatccaata aatcgcggtt ggcggcgcgg ttggtggggt
     3361 tgaagttacc ggcggcgaca aacttataga cgtattcgtt gcgctcatca tcgcccatgt
     3421 aataggccac tcgcaggttg ttattgctat cgcggctgat ggccacctgg gcgctttcat
     3481 gcttgaagcg gcccatggcg gtgcgcttta ccggcacact ggcggggttg ttcgggtcga
     3541 tctccaccac ccaaccgtac aagtgaggct ctaaagggtt ggtgcgggcg ttaaaacgcg
     3601 ggtcggttaa aaaccaattg taagaggcgc c
//
LOCUS       DAOQGS010000423         3622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14542_length_3622_cov_4.921222, whole genome shotgun sequence.
ACCESSION   DAOQGS010000423 DAOQGS010000000
VERSION     DAOQGS010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14542_length_3622_cov_4.921222"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            647..1894
                     /gene="lysA"
                     /locus_tag="PKZ52_13980"
     CDS             647..1894
                     /gene="lysA"
                     /locus_tag="PKZ52_13980"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNG60929.1"
                     /translation="MSNLHTRADGLYFESVSLAELAERFGTPTYVYSRAAITQAYRQY
                     ASALGDWPGQICYAIKANSNLGLLNVLARLGAGFDIVSAGELERVLAAGGDPAKVVFS
                     GVGKTSAEIARGLEVGVGCFNVESESELEAVSQVAVAQNKVASISIRVNPDVNPETHP
                     YISTGLKENKFGIGIDRAVAVYKRAASLPGIRIQGVDCHIGSQLTKLEPFLDALDRLL
                     LLIDELAASGITIHHLDLGGGLGVTYKNETPPSPGDYISAVKARLGERKIALMFEPGR
                     SIVANAGLLLTRVEYLKPTEHKNFAIVDAAMNDNIRPALYQAWQEIVPVVPRNGANKA
                     YDIVGPVCETGDFLGKDRDLCIEPGDLLAMLSAGAYGFVMSSNYNSRPRAAEIMVDGD
                     KAYTVRKRETLADLIAHEQMLPD"
     gene            1900..2730
                     /gene="dapF"
                     /locus_tag="PKZ52_13985"
     CDS             1900..2730
                     /gene="dapF"
                     /locus_tag="PKZ52_13985"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNG60930.1"
                     /translation="MRIKFTKMHGLGNDFVMIDAISQAVTMTPERARKLADRHFGVGC
                     DQVLIVEPPAQPENDFKYRIFNADGSEVENCGNGARCFAVFVRLRRLTHKTEIRAETA
                     GGLLILKVTARGEVCVDMGAPILEPAKIPFLAENQACEYPLEVQGQTLYCGAVSMGNP
                     HAVLLVDNTAAAPVAQLGPLIESHPRFPKRVNVGFMQKLSENEINLRVFERGTGETLA
                     CGTGACAAVVSGIERGLLKSPVKVHLPGGALNISWGGGDRSVEMTGPATVVFHGQVRI
                     "
     gene            complement(2844..3116)
                     /locus_tag="PKZ52_13990"
     CDS             complement(2844..3116)
                     /locus_tag="PKZ52_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60931.1"
                     /translation="MSDEPEELDDDEIEGEPIEEGGDEETVRTASADILEEDDANMAV
                     RNAMRKQIESDVEAFLARGGTISVIPPNVVADPPRKPESNYGGQPI"
     gene            complement(3306..>3622)
                     /locus_tag="PKZ52_13995"
     CDS             complement(3306..>3622)
                     /locus_tag="PKZ52_13995"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNG60932.1"
                     /translation="EHLELALADAEAYIDQIRHAGAIFVGSYTSESLGDYCAGPSHVL
                     PTSGTARFSSPLGVYDFQKRSSVIGCSRRGAQTLGRIASVLARAESLEAHARSAEYRL
                     HE"
BASE COUNT          794 a          935 c          985 g          908 t
ORIGIN      
        1 tctgataatg gtttaacatc tcacaacaca agcgcccaca cgctttgctt gatcattctg
       61 ttaaagaacg ctaagatcgc tcggatcctc gcttttcccg tttcgcttag tgctcagcgg
      121 ggtcgcacat aatactcttt tccaacaccc tgtcaacctt atttttcatc cctcggtgtt
      181 ttttatcgct caattatctt tgaaaaccag ctcagataat tttttgaaca ccgcttctaa
      241 cttactgatt aaaaagactt tttagaaggt tttgcaactt cccattccgc ctttttattc
      301 cgcctcgtcc gaagtggcgc gcattatcta cacctcccct caaccggtca actctttttt
      361 ccaactttat tcccgcgagc tgataattca gccagcagct tgagaattga tttgtctggg
      421 tttccttgtt cttgcgggac aggggcactt atactgcggg gctgttttga ggatttggtt
      481 atgggaactg cgcgctctgc ggctgcgttt ttatgcgttt ttgttggttt gtctctggta
      541 ggttgcgggc aaaaggggcc gcttaagctc cccaataagc cgaccagcca tatcgacact
      601 attcacttgt ttgcttctag tcacttattt gcttctaggt acccctatgt ctaatttaca
      661 caccagagca gacggtcttt attttgagag cgtcagcctg gctgagctcg ccgagcgctt
      721 tggtacgccc acttatgtat atagcagagc ggccataaca caggcctatc ggcaatacgc
      781 cagtgcgctc ggcgattggc ccgggcagat ttgttatgca attaaagcaa actcgaatct
      841 cggcctactg aatgtgttgg ccaggctcgg tgccgggttc gacatagttt cagctggcga
      901 gcttgagcgc gtgcttgccg ctgggggcga ccctgcgaaa gtagttttct ccggtgtagg
      961 taaaacatcc gccgagatcg cccgcggatt ggaggtgggc gtaggctgct tcaatgtcga
     1021 atctgagagc gagcttgagg ctgtatccca ggtagccgtt gcgcagaata aggtcgcgtc
     1081 gatttccatt cgggtcaatc cagatgtaaa ccccgaaacc cacccttata tttccaccgg
     1141 ccttaaagaa aacaagttcg gcatcggcat agaccgggcc gttgctgtgt acaagcgcgc
     1201 ggcaagcttg ccggggatcc gcattcaggg tgttgattgc cacataggct cccaactaac
     1261 aaagcttgag ccattcttgg atgcgctgga caggctgttg ttgctgatcg atgaactggc
     1321 cgccagtgga atcactatac atcacctgga tttgggtggt ggcttggggg ttacctacaa
     1381 gaatgaaaca ccgccctccc ctggcgatta catcagtgcc gtgaaggcca ggttaggcga
     1441 gcgcaaaatc gccctaatgt ttgagcccgg ccgctccata gttgcgaacg cagggctttt
     1501 attgactcga gtggagtatt taaaacccac cgagcacaag aacttcgcca ttgtggatgc
     1561 cgctatgaat gacaatattc gccctgcgct ctaccaggcc tggcaagaaa tagtgcctgt
     1621 ggtgccgcga aacggtgcca ataaagccta cgatattgtg ggcccggtgt gtgagacggg
     1681 ggattttctc ggcaaggatc gtgatttgtg tattgagcct ggcgatttat tagccatgtt
     1741 atcagcgggg gcctatggtt ttgtgatgag ttccaactac aactcccgcc cccgtgcggc
     1801 cgagattatg gtggatggtg ataaggccta cacggtgcgc aagcgggaga cccttgcaga
     1861 tctaattgcc catgaacaga tgctgccgga ttaagcgcca tgcgaattaa gttcaccaaa
     1921 atgcacgggc tgggcaatga ttttgtcatg attgatgcca tcagccaggc ggtgaccatg
     1981 acacctgagc gggcgcgcaa gttggcagat cgccattttg gcgtgggttg cgaccaggtc
     2041 ttgatcgtgg agccaccggc ccagccggaa aatgatttca agtatcgaat atttaacgcc
     2101 gacggcagtg aggttgaaaa ctgtggcaat ggggcgcgct gtttcgctgt gtttgtgcgc
     2161 ttgcggcgct tgacccacaa aacggagata cgcgcggaaa ctgcaggtgg gctactgatc
     2221 ttgaaagtca ccgcaagagg tgaggtttgt gtggatatgg gggcacccat tttagaacct
     2281 gccaagatcc cctttttggc ggaaaaccag gcctgtgagt acccgctgga ggttcagggt
     2341 caaaccctat actgcggcgc ggtcagtatg ggcaaccccc acgcggtgct tttggtggac
     2401 aacactgccg cagcgccggt tgcacaactg gggccgctga tcgaatccca cccgcgcttt
     2461 cctaagcggg ttaatgtggg ctttatgcaa aagctcagtg aaaacgaaat taacctgcga
     2521 gtatttgagc gcggtacagg tgaaaccctg gcctgtggca ccggtgcctg cgcggcggtg
     2581 gtctctggta tcgagcgcgg cttgttaaag tcgcctgtta aggtgcattt acccggcggc
     2641 gcgctcaata tcagctgggg cggaggggat cgctcagtgg aaatgaccgg gcctgccacc
     2701 gtggtgtttc acggccaggt gagaatttaa accgcccctt aaacctaaaa acaatttatt
     2761 gggccgcttg ggcgccaggt taaaaaccct caccacaaaa aaagccgcag gagactgcgg
     2821 ctttttcgta tggaataggc gcactagatt ggctgaccgc cgtagttgga ctccggcttg
     2881 cgcggaggat ccgctacaac attgggcggg atcacgctaa tggtgccgcc acgggccaaa
     2941 aaggcctcta catccgattc aatttgttta cgcatggcgt tgcgcacagc catattggcg
     3001 tcgtcctctt cgaggatgtc agccgatgct gtccgtacgg tctcttcatc gccaccctct
     3061 tcaatgggtt caccttcgat ttcgtcatcg tccagttctt cgggttcgtc actcatatat
     3121 cgctctcaca tgttgcttgg gtgaattaac caggtttgtg gatagttata gaacaagctt
     3181 gaaggcccgt cacgccggaa cgcttgggga cctttagttt attttgttct agcaacaaag
     3241 acgcacaaac acttcggcta tgttactaaa atctacagat tgagtgttct gtagcctggc
     3301 acggctcact catgcaaacg gtattccgcc gatcgcgcat gggcctccag ggattctgca
     3361 cgcgccaaaa ccgaggcaat gcgccccaag gtttgcgccc ctcgacggga acagccgatg
     3421 actgagctgc gtttttgaaa atcatatacg cccaagggcg acgaaaagcg cgcggtgcca
     3481 gaggtgggca atacgtggct ggggcctgcg cagtagtcac caagggattc ggatgtatag
     3541 gaacctacaa atattgcacc tgcgtgacgt atttggtcga tatacgcctc agcatcagcc
     3601 agggccagct ccaagtgctc tg
//
LOCUS       DAOQGS010000424         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14599_length_3614_cov_5.285192, whole genome shotgun sequence.
ACCESSION   DAOQGS010000424 DAOQGS010000000
VERSION     DAOQGS010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14599_length_3614_cov_5.285192"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..699)
                     /locus_tag="PKZ52_14000"
     CDS             complement(388..699)
                     /locus_tag="PKZ52_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HNG60933.1"
                     /translation="MATFHRIAADAALKLLDQTPNALVFDIRAEADYNRGHLAGAQYF
                     TQNQLPMVAKKFSKETPVFIYCYHGNASQTFAQFFIDFRFTQVYSIDGGYEHLSKLVS
                     A"
     gene            complement(1014..1529)
                     /locus_tag="PKZ52_14005"
     CDS             complement(1014..1529)
                     /locus_tag="PKZ52_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60934.1"
                     /translation="MRVCDLVDDPEAVVNSLTEPAASQWRVLSEEPLGVFNQWDRVEH
                     LLCAAQQQAPHELSLSEALYKLYAYSNQFAKAQVFINWVLNQSSRACNLPADPLAASN
                     QHSQQGPLNRAARYYLYALKANAFVALRQGELTRAQALVAQLQLLDPLDQVGGSVVVA
                     LVERMGEEAYA"
     gene            2154..2942
                     /locus_tag="PKZ52_14010"
     CDS             2154..2942
                     /locus_tag="PKZ52_14010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG60935.1"
                     /translation="MAIYSPIDVLNCTTMRFAVADYTISDVLTPAEAAALNQVLPQLK
                     NKAILDLGVGAGRTVAALTAIADSYVGVDYVPEMVAHCRRRFPAVHFEQSDARAMPQF
                     SEQSFDLIVFSCNGICMVDHAGRLAILSEVRRLLRPGGFFMFSTYNRNCKEHNMVFRF
                     PHFRFSPNPLRLLVRSARFAGNTVIRAVNRLRYKRHEVRHADYSILNDQCHNYQTLLY
                     YIDIEGQKRQLQQAGFSGAVSIYDRLGMPAAADINEDMLLYLVS"
     gene            3024..>3614
                     /locus_tag="PKZ52_14015"
     CDS             3024..>3614
                     /locus_tag="PKZ52_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNG60936.1"
                     /translation="MSEFDYLTAGLRTIDAELAAVVALRARLGAPFVQACSLIRATRG
                     RVIVLGMGKSGHIGRKIAATFASTGTPAFFVHPAEASHGDFGMITRDDSVLYISNSGN
                     SPEILTLLPMIKRLNLPLLAMTGNPQSPLAKQAQVVLDISVASEACPLDLAPTSSTTV
                     SVVLGDALAVALLEARGFTAEDFAFSAQKTRSSLFLT"
BASE COUNT          829 a          903 c          950 g          932 t
ORIGIN      
        1 ttgccaaatt caacgacgct agggcgagtg aggtaatttt tcagaggttc cttaactatt
       61 caacactctt tactcccctc tactgcgcgg gcggcaaatt caggaggatt tagccttccg
      121 gcataatcaa ttcgcggtgt agcagtggtg acgataggtt agtgcagacc gtcaaaaaga
      181 gggacctttt tgacgagcct acacggatgt attcacggcg tgtctgcact aacctatcgt
      241 caccactgcg ggcgctggat tacagggtca cagcactaaa acacgcacgc gatagttccc
      301 tgtcagagca agaattaccg caagccacat aaaatgatgc tgaatagtta cagcaatttt
      361 ttcaactgcg atttttaggt taaagtatca ggcggacact aatttgctta ggtgttcata
      421 gccgccgtcg atgctgtaaa cctgcgtaaa gcgaaaatca ataaaaaact gtgcgaaggt
      481 ttggctggca ttgccgtggt agcaatatat aaacaccggt gtctctttgc tgaatttttt
      541 tgccaccatg ggcaattgat tttgggtgaa atactgcgcc cccgctaaat gcccccggtt
      601 ataatccgcc tcggcgcgga tatcaaacac cagggcattg ggggtttggt ccagtaattt
      661 taatgcggca tcggcggcga tgcggtgaaa cgtggccata ggtgcacctg gggcgtgttg
      721 ataaaaggag taagataaaa atggcgagat attcattgct ctagagtaaa aatatacgtt
      781 tttaattaac tcggtggcaa aatagaaggt cctgatctat tttgcctgcg ctgcgctgac
      841 acctgcgaaa acgcctgggt ttttgtgtga tagcctaagc gtttggtggc tggcattcct
      901 gccgctccta taaaatcagt ctaaaaaaca agcccggcct aaaaaacagc cggggttaat
      961 aaataataaa ttgatcgttg cgattctgcg cgcccagcca gtaataatca ggcttaggca
     1021 tatgcctcct cgcccatgcg ctcaaccagc gctaccacta cactgccgcc cacttgatcg
     1081 agtgggtcta ataactgcaa ttgcgcaacc agggcctggg cccgcgttaa ttccccctgc
     1141 ctgagggcca caaatgcatt ggcttttaag gcgtataaat aatagcgggc ggcgcgattt
     1201 aatggccctt gttggctgtg ttggttgctt gccgctaggg gatcagccgg taaattacag
     1261 gctcggctgc tttggtttaa aacccaatta ataaatactt gtgctttagc aaattgattg
     1321 ctgtaggcgt agagtttata cagcgcttcg ctgaggctta attcatgggg cgcctgttgt
     1381 tgggcggcgc ataataaatg ctccacgcga tcccattgat taaaaacccc caggggctcc
     1441 tcacttaaaa cacgccattg ggacgctgcg ggctcggtaa gggaatttac tacggcctct
     1501 gggtcatcaa ctaaatcgca tacgcgcatg gtattaatcc tgattgcaat gagcagctgt
     1561 ttgcaagcct caggccagat taaaaattgt ttaaatgctg gaaaaatagg tattaattga
     1621 cacagcatgt tgtgcgtctg tcgcttatgc gacaggggcc tgcagccttc acgacacagc
     1681 catgcaagga accgctgatt acgtctggcg gcagggatga ttttgcactc gtcgcgggtg
     1741 gcaacgctgt gaggatttac attgcggggc tcccagcacc aagtactcgc ctgatggttt
     1801 taccgtacag ctagtaagtc ggtgcgggaa tcaagtctag ggaacctctg aaaaattacc
     1861 ttactcaccc tggcggcgtt gaatttggca aaaaaatgct catttactgc gtgtaaactc
     1921 cgcttttttt gccaaattcg cctagccaga gcttcgccgc ctacattttt cagaggttcc
     1981 ctagctaaaa taagtccagc taaaataaaa aagtgctgaa tagttagttt gcaaacaatg
     2041 aattttttac cctggcctat actgaaaaat gcagccgcgt aagccgtgta ttgaggtttg
     2101 gactcaggcg catgcactgc tgcacagcca ccgatttatt gtttggatag cccatggcga
     2161 tttattcccc cattgatgtt ttaaattgca ccaccatgcg ttttgctgta gcggattata
     2221 cgatctcaga tgtgttaacc ccagcggagg cagcagcgct gaatcaggtg ctgccacagc
     2281 ttaaaaacaa ggccattttg gatttggggg tgggggcagg gcgcacggtg gcggcgctca
     2341 ccgccattgc cgatagttac gtgggggtgg attatgtgcc ggaaatggtg gcccactgcc
     2401 gcaggcgatt tcctgcggta cactttgaac agagcgatgc gcgcgctatg ccgcaattta
     2461 gcgagcagag ttttgattta attgtgttct cctgcaacgg catttgtatg gtggatcacg
     2521 ctgggcggct ggcaatttta agtgaggtgc ggcgtttatt gcggcccggt gggtttttta
     2581 tgttttccac ttataaccgc aattgcaaag aacacaatat ggtttttcgt ttcccgcatt
     2641 ttcgtttttc gcccaaccct ttgcgcctat tggtgcgctc ggcgcggttt gcgggtaata
     2701 cggtaatacg cgcggtgaat cgcctgcgtt ataaacgcca cgaggtgcgc cacgcggatt
     2761 attccatcct caacgaccag tgccacaatt accaaaccct gctgtattac atcgatatcg
     2821 aaggccagaa gcgccagttg cagcaggccg gttttagcgg tgccgtctcc atctatgatc
     2881 gcttaggcat gcctgcggcg gcggacatca acgaggatat gttgctctac ctggtcagtt
     2941 aaggcccgcc tcgctttggc cactagctga caagcctccg ccgcccctat accatgcccg
     3001 cagtttttcc tgcggcagtt tctatgagcg aatttgatta tctaaccgct gggttgcgca
     3061 ccatcgatgc ggaactggcg gctgtggtgg ccctgcgcgc acgcctaggg gcgccctttg
     3121 tgcaggcctg tagcctgatc cgcgccaccc gcggccgggt gattgtgttg ggcatgggca
     3181 agtccggcca tatcgggcgc aaaatcgccg ccacgtttgc ctccaccggc acccccgctt
     3241 tttttgtgca tccggcggag gccagccacg gtgatttcgg catgatcacc cgggatgact
     3301 cagttcttta tatctccaat tccggcaatt caccggaaat cctcacccta ctgcccatga
     3361 tcaaacgcct gaatttgccg ctgctggcca tgacgggcaa tcctcaatcg cccctggcga
     3421 aacaggctca ggtggtgttg gacatcagtg tggcgagcga ggcctgcccg ctggatttag
     3481 cccccacctc aagcaccacg gtgagtgtgg tgttggggga tgctctggcg gtagctctgt
     3541 tggaggcgcg cggttttacg gcggaggatt ttgctttttc ggcacaaaaa acgaggtcaa
     3601 gtcttttctt gaca
//
LOCUS       DAOQGS010000425         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14659_length_3605_cov_5.494366, whole genome shotgun sequence.
ACCESSION   DAOQGS010000425 DAOQGS010000000
VERSION     DAOQGS010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14659_length_3605_cov_5.494366"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PKZ52_14020"
     CDS             <1..139
                     /locus_tag="PKZ52_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNG60937.1"
                     /translation="HLLISGTGKYHDYNFYRVSGLAIAGKPKGAAQNEAEPVNVKSLL
                     P"
     gene            164..667
                     /gene="nuoJ"
                     /locus_tag="PKZ52_14025"
     CDS             164..667
                     /gene="nuoJ"
                     /locus_tag="PKZ52_14025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNG60938.1"
                     /translation="MGTALAFYLAAAIAIIASVRVITTTNPVHALLYLVVSLLAVAMI
                     FFALGAPFAAALEMIVYAGAIMVLFVFVVMMLNLGSVVVAQEKAWLTPKTWIGPGILS
                     LILLIELCRSIGMETDHPLNTAVIGPKAVGISLFGPYVLAVELASMLLLSAMVTAWHL
                     GRDTEKE"
     gene            671..982
                     /gene="nuoK"
                     /locus_tag="PKZ52_14030"
     CDS             671..982
                     /gene="nuoK"
                     /locus_tag="PKZ52_14030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNG60939.1"
                     /translation="MLETLPMEHGLVVAGILFSLGLAGVLVRRNILFMLMSIEIMLNS
                     TALAFVLAGSRWQQPDGQVMFILVLTLAAAEASIGLALVLQLYRRYQSLDVDAASEMR
                     G"
     gene            979..2826
                     /gene="nuoL"
                     /locus_tag="PKZ52_14035"
     CDS             979..2826
                     /gene="nuoL"
                     /locus_tag="PKZ52_14035"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNG60940.1"
                     /translation="MNHLYLTIVFPLVGFLLLAFGRDRWSENVAAVIGVSALGLSALV
                     AAIAGYDFLTHTPEKGVFVQPLWTWVSIDGFAPTFNLHLDGLSLTMLGVITGVGFLIH
                     LFASWYMRGETDGYARFFSYMNLFVASMILLVLGDNLVAVYFGWEGVGLCSYLLIGFY
                     FRDPANGLAARKAFIITRVGDAFMAIGLCVLFKELGTLHIATLLTEAPKHFASGSPTL
                     AFATLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTYGLF
                     ELTPDVLYLVGLVGAITLVLAGFAALVQTDIKRILAYSTMSQIGYMFLALGVKAYAPA
                     IFHLMIHAFFKALLFLSSGAVILACHHEQNIFKMGGLRKKIPFVYACFVVGGAALAAL
                     PFVSAGFYSKDEILWQAFTTDHKELFYAGVVGAFFTSIYTFRLIFIVFHGKENTHAHA
                     IHGADYWLPLSILLVLSTFVGALIHPPLVGVLPASPEGDEHSKHMVEIISGAIALTGV
                     AVAWFLFIGERKLVTALANSAPGQLLSKLWYSAWGFDFLYDLFLVKPYCLLSRLLRID
                     PVDKSFWLIPAVVGAAHTGLSRVQTGKLRWYATTIVIGAVAVLATLVFL"
     gene            2845..>3605
                     /locus_tag="PKZ52_14040"
     CDS             2845..>3605
                     /locus_tag="PKZ52_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNG60941.1"
                     /translation="MILTWLILIPFLGGFLSWQADRFGALLPRWIALGTMLLLFGFTL
                     NIWLSHDFSLAGLVNGQPKWVLEYQAEWIPHFGIGWHLALDGLSLLMIMLTGFLGVMA
                     VLCSWTEITKFVGFFHLNLMWIIGAVVGVFLAIDLFLFFFFWEMMLVPMYFLVGLWGH
                     SAPGGLGRVQAATKFFIYTQASGLLMLLAIIGLVFAHYSQTGIVSFDYHTLLKTQLSA
                     GLEWTLMLGFFIAFAVKFPIVPFHGWLPDTHSQAPT"
BASE COUNT          617 a         1100 c          933 g          955 t
ORIGIN      
        1 gcacctgttg atctccggca ccggaaaata ccacgactac aacttctatc gcgtttcggg
       61 cctcgccatt gccggcaagc ccaaaggcgc cgcgcaaaac gaagcggagc ctgtaaacgt
      121 gaaaagtctg ttgccctaac acttgccagc tcaggaattt accgtgggaa ctgcccttgc
      181 cttttatctt gccgcagcca tagcgatcat cgcttcggtg cgcgtgatta ccactactaa
      241 tcccgtccac gccctgctct acctggtggt atcgctgctg gcggtggcga tgattttctt
      301 tgccctcggc gccccctttg cggcagccct ggagatgatc gtgtacgccg gcgccatcat
      361 ggtgttgttt gtgtttgtgg tgatgatgct gaacctgggc tctgtagtcg tcgcccaaga
      421 gaaagcctgg cttacgccca aaacctggat tgggcctggc atcttgtcgc tgattctgct
      481 gattgaactc tgccgcagta tcggcatgga gaccgatcac ccgctcaaca cggcggtaat
      541 cggccccaaa gcggtaggga tcagcctgtt cggcccctac gtactcgccg tggagcttgc
      601 ctccatgttg ttactttccg ccatggtgac cgcttggcac ctcggccgcg acactgagaa
      661 ggaatagacc atgctagaga cccttcccat ggagcacggc ctagttgttg ccggcatcct
      721 gtttagcctg gggcttgcgg gcgtactcgt gcgccgcaac atcctgttta tgttaatgag
      781 cattgaaatc atgctgaact ccaccgccct ggcctttgtg ctggcgggca gccgctggca
      841 gcagccggac ggccaagtca tgtttatttt ggtgcttacc ctagccgccg ccgaggcctc
      901 cattggcctg gccttagtcc ttcaactcta tcgccgttac cagtctctgg acgttgatgc
      961 agccagcgag atgcgcggat gaaccatcta tacctcacca tcgttttccc attagttggg
     1021 tttttactgc tggcgtttgg ccgcgaccgc tggagcgaaa atgttgctgc cgttatcggc
     1081 gtaagcgccc tgggcctttc tgccctggtg gccgccattg caggttacga ctttttaacc
     1141 cacacacccg agaagggcgt gtttgtacag cccctgtgga cttgggtttc tatcgacggc
     1201 tttgccccca cctttaacct gcatcttgac ggtctgtccc tcaccatgct gggcgtaatt
     1261 accggcgtgg gctttttgat ccatctattt gcctcctggt atatgcgcgg cgaaaccgac
     1321 ggctatgcgc gctttttctc ctatatgaac ctcttcgtcg ccagcatgat tctgctggtg
     1381 ctgggggata acctggtggc tgtttacttc ggctgggaag gcgtgggcct gtgctcctat
     1441 ttattgatcg gcttttattt tcgcgatccg gccaacggcc tggccgctcg caaagccttc
     1501 atcattaccc gcgtgggcga tgcctttatg gccatcggcc tgtgtgtgtt gtttaaagaa
     1561 ttaggcacat tgcacatcgc cacattgctc acggaagccc ccaaacactt tgccagcggc
     1621 agccccaccc tcgcttttgc caccctgatg ttgttgggcg gcgcggtggg taaatccgcc
     1681 caattgcccc tgcaaacctg gctagcagac gccatggctg gccccacgcc ggtatcggca
     1741 ttaatccacg ccgccaccat ggtaaccgcg ggcgtatatt tgattgcccg cacctacggc
     1801 ctgtttgaat tgacccctga cgttctgtat ctcgtgggcc tagtaggcgc catcacctta
     1861 gtgttagcag gctttgccgc cctggtgcaa accgacatta aacgcatcct cgcttactcc
     1921 accatgagcc aaatcggcta catgttctta gccctcggcg ttaaggccta tgccccggcc
     1981 atcttccacc tgatgatcca cgccttcttc aaggccctat tgttcttgtc atccggtgcg
     2041 gtcattttgg cctgccacca cgagcagaat attttcaaaa tgggcggcct gcgcaaaaag
     2101 attccctttg tgtatgcctg ttttgtggtg ggtggcgcgg ccctggcggc cctgcctttt
     2161 gtcagcgccg gtttctactc taaagacgaa atcctctggc aagccttcac caccgatcac
     2221 aaagagctgt tctacgccgg tgtggtgggc gctttcttta cctcgattta caccttccgc
     2281 ctgatcttta ttgtcttcca cggcaaagaa aacacccacg cccacgccat ccacggcgct
     2341 gattactggt tgcccttgag cattctgctg gtgttatcca cctttgtggg cgcgctgatt
     2401 caccccccgc tagtcggtgt gctgcccgcc agccccgagg gtgatgaaca cagcaaacac
     2461 atggtggaga ttatctctgg cgccatcgcc cttaccggtg tggcagttgc ctggttcttg
     2521 tttatcggcg agcgcaagct ggttaccgcc ttggccaact cggcgccggg ccaactgctt
     2581 agcaaattgt ggtacagcgc ctgggggttc gacttcctct acgatctgtt cctggtcaaa
     2641 ccctactgcc tgctcagccg cctgctacgc attgacccgg tggataaatc cttctggctt
     2701 atccccgctg tggtgggcgc ggcccacaca ggcttgagta gggtgcaaac cggcaagctg
     2761 cgctggtatg ccaccaccat cgtcatcggc gctgtggcgg ttttagccac cctcgtattt
     2821 ttataacttt taggaaccag tcgaatgatc ctcacctggc tcatcttaat tccctttctc
     2881 ggcggcttct tgtcttggca agccgaccgt ttcggggctc tgttgccgcg ctggattgcg
     2941 ctgggcacca tgctgttatt gtttggtttt accctcaata tctggctcag ccacgacttt
     3001 agcctggcgg gcctcgtcaa tggccaaccc aagtgggtgt tggaatacca agccgaatgg
     3061 ataccgcact ttggcatcgg ctggcatttg gccctggacg gtttgtccct gctgatgatc
     3121 atgctcaccg gctttttagg tgtcatggcc gtgctctgct cctggaccga aatcactaag
     3181 tttgtcggct ttttccacct taacctgatg tggatcatcg gtgccgtggt gggcgtattt
     3241 ctcgccatcg acctgttttt gtttttcttc ttctgggaaa tgatgcttgt ccctatgtat
     3301 ttcctcgtag ggttgtgggg ccacagtgca cccggtggct tgggccgcgt acaagcggcc
     3361 actaaattct ttatctacac ccaggcatct ggccttttga tgctattggc cattatcggc
     3421 ctggtgttcg cccactactc gcaaaccggt atcgtctcct tcgactacca caccctgtta
     3481 aaaacccagc tctccgcagg cctggagtgg accttgatgc tgggcttttt tatcgccttc
     3541 gccgtgaagt tccccatagt gcccttccac ggctggctgc ccgacaccca ctcccaagcc
     3601 cccac
//
LOCUS       DAOQGS010000426         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14673_length_3603_cov_5.155862, whole genome shotgun sequence.
ACCESSION   DAOQGS010000426 DAOQGS010000000
VERSION     DAOQGS010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14673_length_3603_cov_5.155862"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKZ52_14045"
     CDS             <1..500
                     /locus_tag="PKZ52_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HNG60942.1"
                     /translation="GALVRLEDQDRALWDQAAITAACGRLRECLSSAPAGPYCLQAAI
                     AACHAEAASFTATPWAEITGLYHALWRREPSPVIALNHAVALSYWQGAEAGLSALAPL
                     AASLGDYYLFYSARAQLHERCQRHPEAAEDYRRALSLVPPGPEHDFLAGRLTQLGSIH
                     LGHSH"
     gene            524..1039
                     /locus_tag="PKZ52_14050"
     CDS             524..1039
                     /locus_tag="PKZ52_14050"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNG60943.1"
                     /translation="MQQPLLELERHFVAPLHRAWQAFTTPQGLAAWMGPAGTQVECRQ
                     FHLAPGGHMHYALISEGAEPFYGRWDFLAVMPFQLLDYYLAFSDAEGNCVHHPLAPGW
                     PLKTRVQVVFQAVADDTRLQINACPAMDSSDEEIACFIASIEHVQANWQEAFSALDEY
                     FAREKASTGRG"
     gene            1187..1672
                     /locus_tag="PKZ52_14055"
                     /pseudo
     CDS             1187..1672
                     /locus_tag="PKZ52_14055"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            1662..2075
                     /locus_tag="PKZ52_14060"
     CDS             1662..2075
                     /locus_tag="PKZ52_14060"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNG60944.1"
                     /translation="MRINVNTQVAAPIDQVWHCYTTPEHIKQWNAASDDWHTTAAEVD
                     LRVGGLFSSRMEAKDGSFGFDFAGTYTQIIPHQLIEYSFGERTARVQFVAGAQGVLVS
                     VEFEAEETHSIEQQREGWQAILDNFARHVTAQTKS"
     gene            2150..2596
                     /locus_tag="PKZ52_14065"
     CDS             2150..2596
                     /locus_tag="PKZ52_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNG60945.1"
                     /translation="MTHATAIFSTERTYPCSCAAVFAAIAEPARLAVWWGPNGFTNTF
                     TEFNFVPNGRWQFVMHGPDGTDFPNESEFLAIEPYKSLQIRHLNAPQFTLTINLSAID
                     GGCLLSWQQAFDDPQVAHAVKAIVEPANEENLARLHRHLQAFGDFD"
     gene            2766..3173
                     /locus_tag="PKZ52_14070"
     CDS             2766..3173
                     /locus_tag="PKZ52_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HNG60946.1"
                     /translation="MISQIYLNLPVANLAKSLAFFKALGFADNPQMSNEAGACVEINN
                     SIFVMLSTRDLFQTFTPKAICDTASAVEVLISLKCASRAAVDSLVAKALAAGGTTYDE
                     PEDLGFMYSHSFVDLDGHGWGLFYAPDEANTVA"
     gene            3309..>3603
                     /locus_tag="PKZ52_14075"
     CDS             3309..>3603
                     /locus_tag="PKZ52_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HNG60947.1"
                     /translation="MPNLTHQQVCINLAVQNVSASQAFFSALGYSFNPEYSNDKAACL
                     ILGANTFAMLLQPDFFNQFAPAGLSNQGNEVLIALSLPDRAAVNDVINKALAAG"
BASE COUNT          837 a          980 c          890 g          896 t
ORIGIN      
        1 gtggcgccct agtgcgcctg gaagatcaag accgcgcact ctgggatcag gccgccataa
       61 ccgccgcctg cgggcggctg cgggaatgct taagcagcgc cccggctggt ccctattgcc
      121 tgcaggcggc catcgccgcc tgccacgccg aggcagcaag tttcacagcg acgccctggg
      181 cggaaattac cggcctctac cacgccctgt ggcgccggga gcccagcccc gtcatcgccc
      241 tcaatcacgc cgtggccctc tcctactggc agggggccga ggctgggctt agcgccctgg
      301 cgcctctggc cgcaagcctt ggcgattact acctgttcta cagcgcccgc gcccaactgc
      361 acgagcgctg tcagcgccac cctgaggctg ctgaggatta ccgccgcgcc ttaagcctgg
      421 ttccccccgg cccggagcac gattttctcg ctgggcgttt aactcagcta ggctcaatac
      481 atttgggcca ctcacactaa ccacagcccc gcaggaaacc tctatgcagc agcccctttt
      541 agagcttgaa cgccattttg tggcgccctt acatcgagcc tggcaggcct ttaccacacc
      601 ccagggcctg gccgcttgga tggggcccgc tggcacccag gtggaatgcc gccagttcca
      661 tttagccccc ggcgggcaca tgcattacgc cctaatcagt gaaggggcgg agccctttta
      721 tggccgctgg gattttttag ccgtaatgcc atttcaactc ttggattatt acctggcctt
      781 tagcgacgcc gagggcaatt gcgtacacca tccactagcc cccggctggc cgttaaaaac
      841 ccgtgtgcag gtggtgtttc aggctgtagc ggacgatacc cgcctgcaga ttaacgcctg
      901 cccagccatg gacagcagcg atgaagaaat cgcctgtttt atagcgtcta tcgaacatgt
      961 gcaggccaac tggcaggagg cctttagcgc cctggatgag tatttcgcgc gggagaaagc
     1021 cagcactggc cggggatagc aaaccggcct aatctaggcc accaataaat attggttaaa
     1081 aaaatgctcg cggctgtcga ttccaagcct agccattcga ctaaccctcg catcgacaca
     1141 ctgcacttat gcagatcttc cggcattcac tgacagtgag caccctatgg ttagtctaaa
     1201 acaaaaaatt ctcacccact tatggtttga tactcaggcg cgggcagcgg cggaatttta
     1261 ttgcggtatt ttcccaaact ctgccataca ccgcatcaac ctactcaaaa acacaccctc
     1321 aggtgattgt gagttggtgg atttccaatt gtgtggccag cgttttatgg ccatcagtgc
     1381 cgggccctat ttcacgttta acgaagcgat ttctctgctg gtgtactgcg acgatcaagc
     1441 ggagatcgat tattactggc aagccttatc agatgaacct gcctctgagt agtgcggttg
     1501 gttaaaggat aaatttggtg tgtcctggca aattgtaccg cgcctaatag acgaagtatt
     1561 tgcccaggaa aataccgaat tacaagggcg cgttttacac gctgtgctcg gtatgacaaa
     1621 aattgattta aaccttttaa aacaactatg tggagacacc tatgcgcatt aatgttaata
     1681 cccaagtggc cgcacccatt gatcaagttt ggcactgtta caccaccccc gagcacatta
     1741 aacagtggaa tgccgcaagc gatgactggc acaccaccgc cgctgaagtt gacttgcgtg
     1801 tgggcggttt attttcctcc cgcatggaag ctaaagacgg cagtttcggt tttgatttcg
     1861 ctggcaccta tacacaaata attccccatc aattaattga atacagtttt ggtgagcgta
     1921 ctgcccgtgt gcaatttgtg gccggtgctc agggcgtatt ggtaagcgtg gaattcgaag
     1981 cggaagaaac ccactccatt gaacaacaac gcgagggctg gcaggcaatt ttggataatt
     2041 ttgctcgcca tgtcacagca cagacgaaat cttaaattct tttgggtatt ttttgcttga
     2101 tcttaattca aattaaacca cttattttaa tgcgccagca ggtaaaacca tgacacatgc
     2161 tactgccata ttctccaccg agcgcaccta tccctgctcc tgcgcagccg tgttcgccgc
     2221 gattgctgaa cctgcgcgtt tagcggtgtg gtgggggcca aatggcttca ccaatacctt
     2281 tacggaattt aattttgtgc ccaatggccg ctggcagttt gtgatgcacg gcccggacgg
     2341 caccgatttt cccaatgaga gtgaattttt agccattgag ccgtataaat cactgcagat
     2401 tcgccacctc aatgcgccgc aatttaccct gacgattaat ttatccgcca ttgatggcgg
     2461 gtgtctgctc agctggcaac aagcgtttga cgatccgcag gttgcccatg cggtaaaagc
     2521 catagtcgag cccgctaatg aggaaaatct ggcgcggctg catcggcatt tacaggcctt
     2581 tggtgatttt gattaagccg gctgcaggta taaaaaagtt catagccagg tcgaaattac
     2641 ctctgccggt tcgattaaat aaagtaagtt ttcatttcag cgaagcggcg gcgcacttga
     2701 gcactgcctt attatctggg cctcagcatt aatcagcagc tttttagcca tgagaggatg
     2761 agattatgat ttcacaaatt taccttaacc tgccggttgc aaacctggct aaatcgctgg
     2821 ctttttttaa agcgctgggt tttgctgata atccgcagat gtctaacgag gcgggtgcat
     2881 gtgtggagat taataacagt atctttgtaa tgttatctac acgggatttg tttcaaacct
     2941 ttacacctaa agccatctgt gacactgcca gcgcggttga ggtgttgatc tcgttaaaat
     3001 gtgcaagccg cgcagcggtg gatagccttg tggccaaggc gcttgcggcc ggtggtacca
     3061 cctatgatga accggaggat ttaggtttta tgtattcaca tagttttgtt gacctggacg
     3121 gccatggctg ggggcttttt tatgctccag atgaggccaa cacagttgcc taattaccac
     3181 cacaaacttg gggcggctat atcttagcgt ttgcagcgcc gcccattggt ttaagcaagc
     3241 tgccatcact ttactccacg cgttaggctc ccaagactaa acatttaatt aattaaagag
     3301 gccttattat gcccaacctc acccaccaac aagtctgcat taatttagcg gtgcaaaatg
     3361 tatcggccag ccaagccttt ttcagcgcgc ttggctacag ctttaaccct gaatacagca
     3421 acgataaagc cgcttgttta atcctcggcg ccaacacctt tgccatgtta ttgcagccgg
     3481 atttcttcaa ccaatttgca ccggcgggcc tttccaacca aggcaatgaa gtattaattg
     3541 ctttaagcct acccgatcgc gccgccgtaa acgatgtaat taataaagca cttgccgctg
     3601 gcg
//
LOCUS       DAOQGS010000427         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14761_length_3589_cov_4.964346, whole genome shotgun sequence.
ACCESSION   DAOQGS010000427 DAOQGS010000000
VERSION     DAOQGS010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14761_length_3589_cov_4.964346"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKZ52_14080"
     CDS             <1..310
                     /locus_tag="PKZ52_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60948.1"
                     /translation="VILALVVTIILSAFFAFQAGQEQAAQQQKNHPGCDLAANKTAGC
                     TVLRDNSTVIATGLLVARSSTHLALYANGKTTIYPLKDYIIDSYKKSNGVIVQASANP
                     "
     gene            446..1042
                     /locus_tag="PKZ52_14085"
     CDS             446..1042
                     /locus_tag="PKZ52_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60949.1"
                     /translation="MDIEMAFKCAASLAAVTTVGIAFYKIKFDWQQRFRDEYNFSLAF
                     IDAVKARVDMHEFCLSKGCHAIMGSSKFKSEEVLHLLSFDAPVEAINDYRFAQGCLSF
                     FRVNGRPRLEYQLGLRTRWIRAFKFWIYLILYAVAALIALSPLLVASKYNTSPADLLY
                     QLSFSVSLFGFIAYCGIRNAKSIHKAERLISAQDRCGL"
     gene            1225..1734
                     /locus_tag="PKZ52_14090"
     CDS             1225..1734
                     /locus_tag="PKZ52_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNG60950.1"
                     /translation="MEYRRVHVDGGTYFFTVTLLERSGNDLLTRHIDLLRQAVAQVKK
                     HYPFKIHAWVVLPDHLHCLLEMPEGDCNYALRWRLIKTLFSRSISADEFRSATRLRRR
                     ERGIWQRRFWEHYVRDQWDFDRHLDYIHYNPLKHGYVKRVADWPHSTFHHFVRCGVYD
                     RGWGDEIAP"
     gene            complement(1868..3409)
                     /locus_tag="PKZ52_14095"
     CDS             complement(1868..3409)
                     /locus_tag="PKZ52_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60951.1"
                     /translation="MALTELSNNSAKVELRDEDQSVLIANYGALITGERSQVLTLALS
                     GLGSSQDNFHIQLTSGSTHNQAPNANCTVSTAYQPYQNKNDTLQNTDCASVTLGKGYS
                     AKYYSIPLKAYTALEVSARGTKFEPLMLVLRDPQGKIIPNYPKSFYSQWDAEYRSTID
                     GTYSLEVISPTPATAINFQLQSQVSSHSYEDYQKTLPHCTIKNIQLPASGTLELNDAF
                     EKSFCIYWQGFSGVSDENKIVYSFTLPPYQKLQIENLNNWEVSEQHYAEMSLESWLHF
                     EYYPYTGLPKATYFNGPAPQQMAISVMQNFGTNAHLRLTTSQAATPKDGKAQSICKTV
                     TSVEVDKISSLGGNLSASSCPSLAKGKDWYANYFSMKLITGSDLYINATFLDYLPANT
                     LFFTLRDSAGKVVPFFYGRSVDFASSAEAHIKKTDTYTLEVTSQEKLTALDYTLYIDC
                     DNGIPKPLPPSEGGSSSGSSSGSSSSGGPDVQAGSVDLLILLGVLGIGLGTRCRSGFS
                     AGKYF"
BASE COUNT          899 a          725 c          931 g         1034 t
ORIGIN      
        1 ggttattctg gcgcttgtgg ttacaatcat tttgtcggca tttttcgcct ttcaagctgg
       61 tcaggagcaa gccgcacagc aacagaaaaa tcacccaggc tgtgacctgg ccgcaaataa
      121 aacagcgggc tgtacggttt tacgcgataa cagcacggtg attgcaactg gccttttagt
      181 ggcccgcagt agcacccatc tggcgctcta tgcaaacgga aaaaccacaa tttacccgct
      241 taaagattac attattgatt cgtacaagaa gtccaatggt gttatcgtcc aggctagcgc
      301 taacccttaa catagtgcgc attggtggtt gcaccgaagt cggaaaatga gggaattatg
      361 gtgttgcaat caggagtcga gttgttgtaa tttattcttg caaggtggta attaatttta
      421 aaatgattgg ttttcgagga tttttatgga tattgaaatg gcatttaaat gcgcggcttc
      481 attggctgct gttacaaccg taggtatagc tttctataaa atcaaatttg actggcagca
      541 gagatttaga gatgaataca atttttctct tgcttttata gatgcagtca aggcgagggt
      601 agatatgcat gagttttgtc tgagtaaagg gtgtcacgcc ataatgggta gttcgaaatt
      661 caaatccgaa gaggttttgc atttgctgag cttcgatgcc ccggttgaag ctataaatga
      721 ctatcgattt gcccagggat gcttaagttt ttttcgcgtt aatgggcgcc caagacttga
      781 ataccagctt ggtttgcgaa ctcgctggat tagagcattt aaattttgga tttatttaat
      841 tctatacgca gttgcagcgt taattgcatt gtcgccattg ttggttgcgt cgaaatataa
      901 tacttcccct gctgacttat tgtatcagct ttcatttagt gtgagtcttt ttggttttat
      961 cgcgtattgc gggatacgta atgctaagtc aatccataag gcggaaagat taatttcggc
     1021 tcaagataga tgtggattat aaaaaggtgt gcttccaata aatggctggt atttatgctc
     1081 ttagtgagca gtaacgcgta acccgtacaa atcgtagggc gcaccttggt gcgccgaata
     1141 agatattttt cacactttaa aacccgcacc aagaagtagg tgcgttatta cctggcgcac
     1201 caaggtgcgc cgtacggatt agccatggaa tatcgacgcg ttcacgtaga cggcggcact
     1261 tattttttca ctgtaaccct gcttgagcgc agcgggaatg atttattaac tcgccatatt
     1321 gacctgctgc gccaagcagt ggcgcaggtt aaaaagcact acccgtttaa aattcacgca
     1381 tgggtggtgt tgccggatca tctgcattgt ttgctcgaaa tgcccgaagg cgactgcaat
     1441 tatgctttac gctggcggct gattaaaact ctattttcgc gctctatttc tgcagatgaa
     1501 tttcgcagcg caacacgttt gcgccgccgg gagcggggga tttggcagcg gcggttttgg
     1561 gagcattatg tgcgcgatca gtgggatttt gaccggcatt tggattatat ccattacaat
     1621 ccgctgaaac acggttatgt aaaacgcgtg gcggattggc cccattcgac atttcatcat
     1681 tttgtgagat gcggggttta tgataggggc tggggcgatg aaattgcgcc ttaagctgaa
     1741 gagtagaggt agggcgcacc ttggtgcgcc gattgaaaat cctttttaga aaaatatcct
     1801 aatttaagat gcgatattgg acttgaacgg cgcaccaagg tgcgccctac gccgctcaat
     1861 accgatttca gaaatattta ccagccgaaa atccgctcct gcagcgggtg cccaagccga
     1921 tccccaacac gcctagcaaa attaataaat ccacactgcc cgcttgaacg tctggccctc
     1981 ctgaagagct gctgccggat gaacttccgc tggatgaacc gccttctgag ggggggagtg
     2041 gttttgggat gccattatcg caatcgatat ataaagtgta atccagtgcg gtgagttttt
     2101 cttggctggt aacttctaga gtataggtgt cggttttttt gatgtgcgcc tcagcggagg
     2161 aggcaaaatc aactgatctg ccgtagaaga acggtactac tttgcctgca ctatcgcgca
     2221 gggtaaaaaa taaagtgttg gccggtaagt agtccaaaaa tgttgcattg atatacaaat
     2281 ccgaaccggt aattaatttc atggagaaat aattggcgta ccagtcttta ccctttgcta
     2341 gtgaggggca gcttgatgct gatagattgc ctccaagcga ggatatttta tctacctcga
     2401 cagaagtaac tgttttgcaa atggactggg ctttgccgtc cttgggggtg gccgcttggc
     2461 ttgtggtgag gcgcaggtga gcattggtgc cgaaattttg cattacacta attgccattt
     2521 gctggggcgc tgggccatta aagtaggtgg ccttgggaag tcctgtgtag ggataatatt
     2581 caaagtgcag ccaggattct aagctcattt cggcgtaatg ctgttcagac acttcccagt
     2641 tgttgaggtt ttcaatctgt aacttttggt agggcggcag cgtaaatgaa taaactattt
     2701 tgttttcatc gctgacgcct gaaaaaccct gccaataaat gcaaaaggat ttttcgaacg
     2761 catcgttaag ctcaagtgtg ccgctggcgg gcagttgtat atttttgata gtgcagtggg
     2821 gcaacgtctt ttgataatct tcgtaggaat gcgaagaaac ctgagattgc agctgaaaat
     2881 tgatggctgt tgcgggggtg ggggaaatta cttctaagct gtaggtgcca tctattgtgc
     2941 ttctatactc tgcgtcccat tgggagtaaa aggatttggg gtaattgggg ataatcttgc
     3001 cttggggatc tcttaatacc aacatgagtg gctcgaattt ggtgccacgg gcggaaacct
     3061 ccagcgctgt gtaggctttt aagggaatag aataatattt tgccgaataa cccttgccga
     3121 gcgtgacgga tgcgcagtct gtgttctgca atgtgtcatt tttattttga tagggctggt
     3181 aggcggtgct aaccgtacaa ttggcgttgg gggcctggtt atgagtggag ccgctggtca
     3241 attggatgtg aaaattatct tgcgagctac ctaggccgct taaagcgagc gttaatacct
     3301 gcgagcgctc gcccgtgatt aaggcgccat agttggcaat gagcacggat tggtcttcat
     3361 cgcgcaattc tacttttgct gaattgttgg ataattcagt taacgccaca aaactgttgg
     3421 ctggaacctc aaaactaaag cgtgtacagg ataaaaaacc ggtgatgcct gcaaaaatat
     3481 aactgccttc gctcgatagc tctacctgct ctgcgggcgt tgtgcaggat ctaaggaacc
     3541 tctgaaaaat tacttaaaat tggtaaaatg gcatctttcc taccgccgg
//
LOCUS       DAOQGS010000428         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14855_length_3574_cov_5.103154, whole genome shotgun sequence.
ACCESSION   DAOQGS010000428 DAOQGS010000000
VERSION     DAOQGS010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14855_length_3574_cov_5.103154"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1904)
                     /locus_tag="PKZ52_14100"
     CDS             complement(210..1904)
                     /locus_tag="PKZ52_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNG60952.1"
                     /translation="MLARVGTYTLAGAELETVLIPNETTQYEPSQFQGSAYLYNGSNI
                     TPERIYRFPGKAHTMEYAGWSVALSNQWAAFATRPYVSSAPGVQNDPTQTYVYIVGKT
                     NGVWNSCPTENSLANNCNNAFRNNGVALTKPLTRIAFGTKSSLPENLDANSFAMAISD
                     KYLVLANSRNSIVNIYRYNSTSKNWDLEFNLDDDDYKTVGRAVAISGDKVAITSSWMP
                     ENQSTPGVFNGYVRIYHRNATTGTWSMTSQADGLIAGGNYGYRVKMEGNNLVVAAGTE
                     EAHRLYFYRFDSNDRINGSPFVVPFSGRIRGLSLSGETVAVAGGDNNYALSVFSRNTL
                     NSAPEWKRSTSLAGSFYSNRNFDGQPYQGIDEVEVVGDDLSLGWRAHNSTTQPNNFKG
                     AVIFEKVSLLDSCREPTNLVANCSFDAVTNNSLNTAASGANWSLLSYLGASGSVDYTG
                     RQMRVSINNPGSDMWHVQARTAVNLSQAVNYVLTFRAKADNNRGFVVNIGHNGNQDNN
                     WQSYGRVNVSATTEWTEYSYEFSGVPMDANAFLDFNFGNAGTSAVTIDSVKLKALN"
     gene            2408..3352
                     /locus_tag="PKZ52_14105"
     CDS             2408..3352
                     /locus_tag="PKZ52_14105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG60953.1"
                     /translation="MNTSISSLQFDWTLIRSFLAVMDEGNLQAAAARLHSTQPTISRH
                     IATLEQQLGSRLFDRRARQLSPTALAVNIALHARDMESNANAISRALVAQNDNLITQV
                     RISASQCTSCYILPPILKMLRAQEPGIAIELISSNHYVSLQKLEADIAVRMARPQEEC
                     LVARRLGQVNIGAYAHKNYLAERGTPKTAADLMQHDLIGYDQQLSILKGLQQWGLAIE
                     RAQFSLRTDDHMMVWQAIHQGLGVGFTAHYVARRDPDVLPLLTHLPVEPMEVWLTLHE
                     DLRTSVGIRRVFNCLAEHLTERLALEAKIDQTLNAAHI"
BASE COUNT          912 a          854 c          843 g          965 t
ORIGIN      
        1 cggtctgtca gtcggagcca aattgccacg ccgtttttgc cttggctaac accaagccga
       61 tccgcagttc ggtccgttta aaaatgtgat aaatggggtt cgctatttta aaacccaata
      121 aaaaggcccc tcaggggcct ttttattggg tttttaacct aaaaatagca gtgggtgtta
      181 actgctattt ttaggtttaa taactgtttt taattcaacg ctttcaattt aacgctatca
      241 atggtgaccg cactggtacc ggcattacca aaattaaaat ctaaaaatgc attggcatcc
      301 atgggcacgc cgctaaattc ataggaatat tccgtccatt ccgtggttgc actcacattc
      361 acgcggccat aactctgcca gttattatcc tggttgccgt tgtggccgat attcaccaca
      421 aaaccgcgat tgttatcggc cttggcgcgg aaggttaaaa cataattcac cgcctgggat
      481 aaattaaccg ctgtacgcgc ctgcacatgc cacatatcag aacctgggtt attaatgctc
      541 acgcgcattt ggcggccggt gtaatcgaca ctgccagagg cgcccagata gctaagcagg
      601 ctccaattcg cgccgctggc ggcggtattg agtgaattat tcgtaactgc gtcaaacgaa
      661 caattggcca ctaaattagt aggttccctg caggaatcaa gcaggcttac tttttcaaat
      721 ataaccgcgc ctttaaaatt attcggttgc gtggtgctgt tatgggcgcg ccagcctaag
      781 gataaatcat ctcccaccac ttccacttca tctatacctt gatagggctg cccatcaaaa
      841 ttgcgattgc tataaaaact gcccgccagt gatgtggagc gtttccattc cggcgcacta
      901 ttaagtgtat tgcggctaaa tacactgagc gcataattat tatcgccacc ggctacggcg
      961 actgtttcac cgcttaaact caagccgcga attcgaccgc taaacggcac cacaaatggt
     1021 gagccgttga tgcgatcatt gctatcaaag cggtaaaaat acaagcgatg agcttcctct
     1081 gtgcctgctg ccaccactaa attattgccc tccattttga cgcggtagcc gtaattacca
     1141 cccgcgatta aaccatcagc ctgtgatgtc atactccagg tgccagtggt tgcattgcgg
     1201 tgataaatgc gcacataacc gttaaataca ccgggcgtag attgattttc cggcatccag
     1261 ctggaggtta tcgccacttt atcgccggaa attgccactg cgcggccgac ggttttgtag
     1321 tcgtcgtcat ccaaattaaa ttctaaatcc cagtttttgc tggtgctgtt atagcggtaa
     1381 atattcacaa tgctattgcg tgaattggcc agcactaagt atttatcgga aatcgccatg
     1441 gcaaatgaat tggcatccag attttccggc agggaagatt tagtgccaaa ggcaatacgg
     1501 gttagcggtt tggtaagtgc aacgccattg ttacgaaatg cattattgca attattggcc
     1561 agtgaatttt ccgtgggaca gctgttccac acgccgttgg ttttgccaac tatatacacg
     1621 taggtttgcg tgggatcgtt ttgcacaccc ggcgcactgg atacataagg ccgcgtggca
     1681 aaggccgccc actgattaga aagcgcaact gaccagcctg catattccat ggtgtgggct
     1741 ttgccgggga agcgataaat gcgctcaggt gtgatattgc tgccgttata taaatacgcc
     1801 gagccctgaa attggctggg ttcgtattgg gtggtttcat tggggattaa tacggtttct
     1861 aactctgcgc ccgccagcgt ataagtgcct acacgcgcga gcattctgcc catttctggg
     1921 ttttgtgaac catggggtgc cggtttgggg gcaacaatac ccgcctgcgc actcagggct
     1981 gttaacgcgc tgatgcttaa agctagtatc catggggcgt gaaggcgagg ttttggttgt
     2041 atctgggtca taaatatctc gtggcgtgta gattaaatat ccccaattta attatgggga
     2101 actgtcgcag ctgctgcgag caatctaaca agcgtctaat ttcctgtgca atgtaatttc
     2161 atctgcaaaa atgcatatgt aaaattcatt tttgtacagc acacttaggt tgcgcaaatg
     2221 tataaattcg gttaggcgtg ctatgtattt accaatattt atcttattat tggccttaag
     2281 tgaatatcga aagtaggttg cagtggtgaa atttaggcaa atttaaattg aaagcggact
     2341 ggttttttta ccgttgcgac aaatggccca gcaaattatt agttattaaa taatgtgaga
     2401 actcgccgtg aatacgtcta tatcatccct tcaatttgat tggaccctta tccgttcatt
     2461 tttagcggtt atggatgagg gtaacctgca ggcagctgca gctaggctac attccacaca
     2521 gccgacgatt agccggcata ttgccacctt agagcagcaa ttgggcagcc ggttattcga
     2581 tcggcgcgcg cgccaattat cgccgacagc cttagcggtt aatatcgcgc tacacgcgcg
     2641 cgatatggaa tccaacgcca atgctattag ccgagcacta gtggcacaaa atgataatct
     2701 gattacccaa gtgcgcatta gcgccagtca atgcacgtcc tgttatattt tgccgcccat
     2761 tttaaaaatg ctcagagcac aagaacccgg catagccatc gaattaattt cctctaatca
     2821 ttatgtcagc ctgcagaaac ttgaagcgga tatagcagtg cgcatggccc gcccccagga
     2881 ggaatgttta gtggcccgcc gcctcgggca ggtgaatata ggtgcctatg cccataaaaa
     2941 ttatttggcc gagcgcggca cgcctaaaac cgccgccgat ttaatgcaac atgatttaat
     3001 cggttacgat caacaactgt ctattttaaa aggcttgcaa cagtgggggc tggctattga
     3061 gcgcgcgcaa tttagcctgc gcaccgacga tcatatgatg gtatggcagg ctatccacca
     3121 gggtttgggc gtaggtttta ccgcccatta tgtcgcgcgc cgagaccccg atgtgctgcc
     3181 attgctcacc cacttaccgg tcgagcccat ggaagtttgg ctgacattac atgaggattt
     3241 gcgcactagt gtaggtattc gcagagtatt taattgcctg gcggaacact taaccgagcg
     3301 tttggcgctg gaggctaaaa tcgatcaaac attaaatgcg gcgcatattt aacagcctgt
     3361 ggaaatgcct gtgcggcgat gctctggcaa gaaaaattca acgccaggga gatattatcc
     3421 taaaaaccgc agttgaactc tactacggct gcctaatact tgaatctgca cgcttttttg
     3481 tcaaaatttg actcaccgta gtggcgacta ctgctttcac caaattttga caaaaaagcg
     3541 cccatattca gcgtcttagg caccctcgct acga
//
LOCUS       DAOQGS010000429         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14919_length_3564_cov_4.905101, whole genome shotgun sequence.
ACCESSION   DAOQGS010000429 DAOQGS010000000
VERSION     DAOQGS010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14919_length_3564_cov_4.905101"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKZ52_14110"
     CDS             <1..427
                     /locus_tag="PKZ52_14110"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNG60954.1"
                     /translation="VVLLGYSFGADVLPYVAANLPKEQQAAVKHLVLLGLSQFAHFEF
                     YLTNWLSSETKASPFATQDALKSIPAIAGLCVQGAEDDESICSQLQQPNIKSVTLPGD
                     HHYDEDYDSLARIILTVLAGGEAQAVKPGATDSAAKQVN"
     gene            578..>3564
                     /locus_tag="PKZ52_14115"
     CDS             578..>3564
                     /locus_tag="PKZ52_14115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNG60955.1"
                     /translation="MYHNTVAPSFLAQLSATMKQQPQAQALVLVDEVWSYEQLDQTAN
                     RLQFYLADAGLSRGAVVAIHTHSRAIAIAAMIAILREGGAYLPLDPLYPPERLQYMVE
                     NASCRLMLCDNDDITPAGITRVDVRSLDMSLPLPKLALAPIKAESDAYIIYTSGSTGR
                     PKGVRMNHGTLNNLIHWQNQHYIEGECYRTLQFSALSFDVSFQEIFATLCQGGTLVLL
                     PPEIKQDFRALLTFVDQQGVERLFLPYIALLQLIQWANRLEFYPECIREWITAGEQLV
                     VSDELRRAFTSMPYARLINQYGPSESHVVTELILPEDPSLWPAVPSIGYPIDTAQLWV
                     LDDEGRELADLEEGELWIGGPVLGNGYINNPEETAKRFILREMDGEPQRIYRTGDLAS
                     KNRYGEISYKGRIDAQVKISGYRVELAEIEAQMLNTGAVREVAVAVEEQGDIKRLVAF
                     MVDGAMPWSDEQLFASLHKALPFYMVPSQILRLPALLKTPSGKVDRKSLLAQLSQFEQ
                     AQLPHSSGEHGPAVETIALEEAPITGAASLEVPNSSGPTEAQILTILRRELLLPGLTL
                     EDNLVAMGMTSLGANRVAAAFYDDLGLDLPTYTLFQHRNVARLVAALTGVRAAGSPAP
                     GAKLEAQSQAAIAIVGMALRVPGAQDVLTFWRNLVNGQESIHFFEPTQNGRVNARGVL
                     AEPLAFDEQFFDIPPVEAKFIDPQQRLLLELAWQALENAGVVPEQFTGRIGVYCGTGN
                     NTYYLERVLQNPDALENYSSFAAMLANEKDYCATRIAYKLNLVGPAVSVHTACSTSLV
                     AVCQAVEALRQGHCDIALAGGAALTFPQQQPHEHQEGSIYSADGHTRTFDAASSGTVF
                     SDGGAMVVLKRLDYALADGDHIYASIKGIGINNDGADKGSFSAPSLEGQRRVIGQALL
                     DAGVNAAAIGLVEAHGTATPVGDPIEVAALTQAYREHTDEVGYCHLGSLKSNFGHLTA
                     AAGVAGLIKTALCVEQGARVASI"
BASE COUNT          722 a         1016 c         1065 g          761 t
ORIGIN      
        1 ggtggtgttg ctcggctatt catttggcgc cgatgtgctg ccctatgtgg ctgcgaattt
       61 gccaaaggag cagcaggcgg cggtgaaaca cctggtgtta ctggggcttt cgcaatttgc
      121 tcattttgaa ttttatttaa ccaactggtt atccagcgaa accaaagcat cgccttttgc
      181 cacccaggat gcgctcaagt ctatccctgc tatcgccggt ttgtgtgtgc agggcgcgga
      241 ggacgacgag agtatctgca gtcaattgca gcagccgaat atcaaaagcg ttaccttgcc
      301 cggggatcat cactacgatg aagactacga tagcctggcg cgcatcattc tgacggtact
      361 tgccgggggt gaggcacagg cggttaaacc tggagcgacg gacagcgcgg ccaagcaggt
      421 taactaacct gtgctttatg gcagttctag tgggttgcgc taaggtaata ttgccatttg
      481 cagtgtgtga ggtttttttt ccgcgcagtt tttagcacac tggcgcccta gtctgccttt
      541 tagaaggcgt ttgccataac caccatggaa ccctggtatg tatcacaaca cggtcgctcc
      601 atccttcctt gctcagttgt cggcaaccat gaaacagcag ccccaggcgc aggcgctggt
      661 gttagtcgat gaggtatgga gttacgagca gctcgaccaa actgccaatc gcctgcagtt
      721 ttatcttgcc gatgccgggt tgagccgagg cgccgtggtg gcgattcaca cccattcgcg
      781 agcaatagcc atagcggcga tgattgcaat actgcgcgaa ggcggcgcct atttgcccct
      841 ggaccctctc taccccccag agcgtttgca gtacatggtg gaaaacgcta gctgccgcct
      901 gatgctgtgc gataacgatg acataacccc agcgggcata acacgggtgg atgtacgcag
      961 cctggatatg agcctgcccc tgcctaaact agccttagcc cccataaaag ccgagagcga
     1021 cgcctacatc atttacacct ccggctccac cggcaggccc aagggggtgc gcatgaacca
     1081 cggcaccctg aacaatttaa tccattggca aaaccagcat tacatcgagg gcgagtgtta
     1141 ccgcaccctg cagttttcgg cccttagttt tgatgtctcc ttccaagaaa tttttgccac
     1201 cctctgccag ggcggcactt tggtgctgtt gccgccggag atcaaacagg attttcgcgc
     1261 cctgttaacc tttgtggatc agcagggggt ggagcgttta ttcctaccct atattgcgct
     1321 gttgcagttg atccagtggg ccaatcggct ggagttttac cccgagtgta tccgcgagtg
     1381 gatcacggcc ggggagcagc tggtggtgag tgatgagctg cgccgggcgt ttacgagcat
     1441 gccctacgcc cgcctgatca accagtacgg tccctctgaa tcccatgtgg taaccgagct
     1501 gattctgccg gaagacccca gcctctggcc tgcggtgccg agtatcggct accccatcga
     1561 taccgcccag ttgtgggtgt tggatgacga gggtcgggag cttgccgatt tagaagaggg
     1621 ggagttgtgg atcggcggcc cggttttggg caatggctac attaataacc cggaggaaac
     1681 cgccaagcgg tttattctgc gggagatgga cggggagccc cagcgcatct accgcaccgg
     1741 ggatttagcc agcaaaaacc gctatgggga gatcagttac aagggccgca tcgacgccca
     1801 ggtgaagatc agtggctacc gggttgagct tgccgagatt gaggcgcaga tgctcaacac
     1861 cggcgcggtg cgggaagtcg ctgtcgcggt ggaggagcag ggggacatca aacgcctggt
     1921 ggcctttatg gtggatggcg ccatgccctg gagcgacgag cagctgtttg cttctctgca
     1981 caaagccctg ccgttttata tggtgccaag ccaaatcctg cgtttgcccg cgctattaaa
     2041 aacccccagc ggcaaggtgg atcgcaaaag cctgctagcg cagttaagcc aatttgaaca
     2101 agcgcagctc ccgcacagct ctggggagca tgggcctgct gtcgagacta tcgccctgga
     2161 agaggcgccc atcacaggtg cagctagtct agaagttccc aactctagtg gcccgacgga
     2221 ggcgcagatc ctcaccattc tgcgccggga gttgctgctg cccggcctta cccttgagga
     2281 caatttagtg gccatgggca tgacctccct gggtgccaac cgggtggccg ccgcgttcta
     2341 cgatgatttg gggctggatt taccgacgta cacgcttttt caacaccgca atgtggcccg
     2401 cctggtggcc gcgctcaccg gcgtgcgagc agcaggcagc cccgcccccg gcgccaaatt
     2461 ggaggcccag agtcaggcgg ccatcgccat agtgggtatg gccctgcggg tgccgggcgc
     2521 acaggatgtg cttaccttct ggcgcaatct ggtgaatggc caggagtcca tccacttttt
     2581 tgagcccacc cagaacggtc gggtcaatgc ccgtggtgta ttggcagaac ccctggcctt
     2641 tgatgagcag ttttttgata ttccgccggt ggaagccaaa tttatcgacc cccagcagcg
     2701 cctcttattg gagctggcct ggcaggcatt ggaaaacgca ggggtggtgc cggagcagtt
     2761 tactggccgt atcggggtgt actgcggcac cggcaacaat acttattacc tcgagcgggt
     2821 gctgcaaaac ccggatgcgt tggaaaacta cagcagcttt gccgccatgc tcgccaacga
     2881 aaaagactac tgcgccaccc gtatcgccta taagctcaac ctggtggggc ctgcggtgag
     2941 cgtgcatacg gcctgttcca cctcgctggt ggcggtttgc caggcggtgg aggccctacg
     3001 ccagggccac tgcgatatag cgctggcggg cggcgcggct cttacctttc cccagcagca
     3061 gccccatgaa caccaagagg gcagtattta ttccgccgac ggccacaccc gcacctttga
     3121 tgcggcaagc tccggcaccg tgtttagcga tggcggcgcc atggtggtat taaaacgcct
     3181 ggattacgcc ctggctgatg gcgaccacat ctacgccagt atcaaaggca tcggtattaa
     3241 caacgacggc gccgataagg gcagtttttc cgcccccagt ttggaaggcc agcgccgggt
     3301 gatcggccag gccctgctgg atgcaggcgt gaatgccgct gccataggtt tggtggaggc
     3361 ccatggcacc gccaccccgg tgggggatcc cattgaagtg gccgccttaa cccaggccta
     3421 ccgagagcac accgacgagg taggctactg ccacttaggc tccctaaaaa gtaactttgg
     3481 ccacctaacc gccgctgccg gtgtggcggg cttaattaaa acggccctgt gtgtggagca
     3541 gggcgctcgg gtggccagca ttca
//
LOCUS       DAOQGS010000430         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_14995_length_3552_cov_5.497855, whole genome shotgun sequence.
ACCESSION   DAOQGS010000430 DAOQGS010000000
VERSION     DAOQGS010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14995_length_3552_cov_5.497855"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..2637
                     /locus_tag="PKZ52_14120"
     CDS             286..2637
                     /locus_tag="PKZ52_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60956.1"
                     /translation="MANFNSYGFSAWRQRWLDSPWLQRFVQLGLARQAMLLGLMLLAV
                     IVLSGLAGIGLAVAHGRSHMLNQWAADEVHKREAALIAAVGSGVNEAVTSRLQYFVEA
                     GFAAGVRLHQRSTLRQWQVPAGLQAAAPLLEFSLGPEGDLPAPQWLAAPWPVGISLLW
                     LALISGLCALWAFLVWWMYVRPVITLAQHAHNLSMDRVAPIKPAITQPVLLTELADVY
                     NALEHVRHNLHEELEQRKAVELALMAEKHEKLGVRRQQMVAEQANRAKSQFIATMSHE
                     IRTPMNGILGMVELLRDTDLTPAQNHYLDVINRSGDNLLVIINDILDYSKIEAGKMEL
                     EEHSFLLDDVVDDSAALFAAMANKRNVELLTSIEPGVPRNLIGDPMRLQQIVVNLVGN
                     AFKFTKAGYIHLHVRLFQDEQPQAPKLLFSVSDTGIGIERHIKDSLFEAFSQADSSTS
                     RHYGGTGLGLTISKQLANMMGGEIGVDSTPGQGANFWFTAQFRLNNQAPTFNADLVLL
                     RQKQLLLISENTRLASIIQQQCAYWQMPCTYAHNRVEVWAALERMNTSGQSFHAVLVD
                     AKMAEGFPSELVWQIKERYPNYPWRFILLARNQHTLEADANFDHLITRPIAMRRLEAA
                     LLGRYGPTVNHEAGLIAQDSILLPMHNPSDIKLLVAEDNPVNRMVIEGLLRKLGIQPD
                     FVEDGLAAVNAATDNTKRYDVVLMDCEMPQLDGFEASMRIRAWEEQHDYAPLSIIALT
                     AHIELEHKERVFDIGMNYFLSKPITLDKVREALIRLGFLARGV"
     gene            complement(2654..3247)
                     /locus_tag="PKZ52_14125"
     CDS             complement(2654..3247)
                     /locus_tag="PKZ52_14125"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNG60957.1"
                     /translation="MKIASFGLVAALVLSAPSFADWKLQADQSQLFFSSVKKDIIAET
                     HQFKTVQGVVSEDGQFSVSVDLASAETGIPIRNERLAQFLFETTKFSQASAKGKIDLA
                     ALGKQTIGSSQTVKLPVIFELHGKTITQDASLTLTKLSASQVAVVTSAPIFLNAADFD
                     LLPGIEKLKELAALPSISPLVPISLHLRFTHEASKKG"
BASE COUNT          834 a          860 c          960 g          898 t
ORIGIN      
        1 tttaagggct gcgccgggtt ttgccaaacc ttgcacccca gagtctggcc gccgcccatt
       61 gggaatttcc acccatcgag ctgttcacag acacccagtt tacaactggg cggcttttgg
      121 gctcacgatg cttattaatc aaggtcggct aaaacccgcg cccgccctct gcgcctatta
      181 aataatggcg agaatttttt tcctggcagc gtcaaaactg tcgtcggctg cgaggaagcc
      241 gctgctcttg ttacactagg gccgtctttt tagcgcgtat tactcatggc caattttaac
      301 tcgtatggat tttccgcctg gcgccagcgc tggctggatt cgccttggtt gcagcgcttt
      361 gtgcagctgg gattggctcg gcaggcgatg ctgttggggc tgatgctgct ggcggtaata
      421 gtgcttagcg gccttgcggg cataggtttg gcggttgcgc acgggcgcag ccatatgctc
      481 aatcaatggg cggcggatga ggtgcacaag cgggaggcgg cgctgatagc cgcagttggc
      541 tcgggtgtaa atgaggccgt taccagccgc ctacagtatt ttgtggaagc cggttttgct
      601 gcaggcgtgc gcctccatca gcgcagtacc ctgcgccaat ggcaagtgcc cgctggcctg
      661 caggcggcgg cgccgctgtt agagttttcc ttaggcccag agggcgatct ccccgccccg
      721 caatggctgg cagccccttg gccggtgggc ataagcctgc tttggctggc gctgattagc
      781 gggctgtgtg cgctgtgggc gtttttagtc tggtggatgt atgtgcgccc ggtaataaca
      841 ctggcccagc atgcccacaa cctgagtatg gatcgcgtcg cgcctataaa acctgccatt
      901 actcagccgg tgctgctcac tgaattagcg gatgtgtaca acgcgctaga gcatgtgcgc
      961 cacaatttgc acgaagaatt agagcagcgc aaagcggtgg agcttgcgct gatggcggaa
     1021 aaacacgaaa aactcggtgt gcggcgccaa cagatggtgg ccgaacaagc gaaccgcgcc
     1081 aaaagccagt ttattgccac catgagccac gaaattcgca cgcccatgaa cggcatttta
     1141 ggcatggtgg aattattgcg cgatacagat ttaactccag cgcaaaacca ttatttggat
     1201 gtgattaacc gctcgggtga taatttattg gtgatcatca acgatatttt ggattattcc
     1261 aaaatcgaag cgggcaaaat ggaattggag gagcacagct ttttgctcga tgacgtggtg
     1321 gatgatagcg ccgcgttatt tgccgctatg gccaataaac gcaatgtgga gttattaacc
     1381 agtattgagc caggcgtgcc gcgcaacctc attggcgacc ctatgcgcct gcagcagatc
     1441 gttgttaacc tggtgggcaa tgcctttaaa tttaccaaag ccggttatat tcacttgcat
     1501 gtgcggttat ttcaggatga acaaccgcag gcgcctaagc tgttattttc ggtcagtgat
     1561 accggtatag gtatcgagcg ccatattaaa gattctttat tcgaagcctt tagccaagcc
     1621 gatagctcca ccagccgcca ctacggcggc acgggtttag ggcttaccat ttccaaacaa
     1681 ttggccaata tgatgggcgg tgaaattggt gtggacagca caccggggca gggcgccaat
     1741 ttttggttta ccgcacaatt tcgtttaaat aaccaggcgc cgacatttaa tgccgacctg
     1801 gtgttattgc gtcaaaaaca gctgctgttg atcagcgaaa atactcggct ggccagtatt
     1861 attcagcagc agtgcgccta ttggcagatg ccctgtacct acgcacacaa tcgcgttgag
     1921 gtgtgggcag cgcttgagcg catgaataca tccggccagt catttcacgc ggtgctggtg
     1981 gatgccaaaa tggccgaggg ttttccttca gaattggtgt ggcaaattaa agagcgctac
     2041 cccaattatc cctggcggtt tattttattg gcccgcaacc aacacacgct ggaggcggac
     2101 gctaattttg accacttgat taccaggccc atcgccatgc ggcgtttgga agcggcccta
     2161 ctggggcgct atggccctac cgttaaccat gaagcgggcc taattgctca ggattcgatc
     2221 ctgttgccca tgcataatcc gtcggatatt aaattattag tggcggagga taatccggtg
     2281 aatcgcatgg taattgaagg cctgctgcgc aaattgggca ttcaaccgga ttttgtggaa
     2341 gacggtttag cggcagtaaa tgccgccacc gataatacta agcgctacga tgttgtttta
     2401 atggattgcg agatgccgca attggatggc tttgaagcca gtatgcgtat tcgcgcctgg
     2461 gaggaacaac acgattacgc cccgttaagc attattgccc tcaccgcaca tatagagttg
     2521 gaacacaaag agcgcgtatt tgacatcggt atgaattatt ttttaagtaa acccatcacc
     2581 cttgataaag tgcgcgaagc gttaattcgg ctgggttttt tagccagggg tgtataaccc
     2641 gcccggtaaa aaattaacct tttttgctcg cctcgtgggt aaaacgcaag tgcaacgaaa
     2701 tcggcaccag tgggctgatt gaaggcagcg ccgccaactc ttttaatttt tcgatgccgg
     2761 gtaataaatc aaaatcggcg gcatttaaaa atatcggtgc cgaagttaca acagcaacct
     2821 gggaggcgct taatttagtc aaagtaaggc tagcatcttg ggtaatggtt ttaccgtgca
     2881 attcaaaaat caccggcagt tttactgttt ggctgctgcc aatggtttgt ttgcctaggg
     2941 cggccaaatc aattttgccc ttggcgctgg cttggctgaa tttagtggtt tcaaataaaa
     3001 attgcgccaa gcgctcattg cgaataggga tgccggtttc ggcactggcc aaatctacac
     3061 tcaccgaaaa ttgcccgtct tcactcacca caccttgcac ggttttaaat tgatgggttt
     3121 cggcgatgat gtcttttttg acggaggaga aaaacagttg ggattgatca gcctgtaatt
     3181 tccaatcggc aaaactcggt gcggataaaa ccagtgcggc cactaggccg aaggatgcaa
     3241 tcttcataaa tgggctccaa aaaatactag gttaagtgtg gcaattaacc ataaatctta
     3301 agcggttaat catagggtga acgccccaca ataacttttt catttcactg ggacaaggct
     3361 tttgccgcga gagccggcaa aacaccactg gacttgggct gttttggttt atctgtggca
     3421 atttcaacag gttgttaagg aacctctgaa aaatgtagcc agcgttttgc atacgctgtg
     3481 ctaaacctac gaaaatgcgc ggattttctt gttttagcgc agctcccggc ggtttacacc
     3541 gccggggcgg ca
//
LOCUS       DAOQGS010000431         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15016_length_3548_cov_5.530490, whole genome shotgun sequence.
ACCESSION   DAOQGS010000431 DAOQGS010000000
VERSION     DAOQGS010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15016_length_3548_cov_5.530490"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(579..1085)
                     /locus_tag="PKZ52_14130"
     CDS             complement(579..1085)
                     /locus_tag="PKZ52_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HNG60958.1"
                     /translation="MFFSTRLKAVFNIGAALALMVGAQAWAAELTPNVFSIALKSLQA
                     PETTSLSQFHNKSLVVSFFEPDCPWCYRQILMLNDVQQRCSDQLQVILIGTQGTRQQL
                     QNELRRSKTLLPAFASSSALLQLTGPIRATPVTFIFNGQGVKVAELKGFAPQSAILDG
                     ICRAQVVP"
     gene            complement(1075..2964)
                     /locus_tag="PKZ52_14135"
     CDS             complement(1075..2964)
                     /locus_tag="PKZ52_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG60959.1"
                     /translation="MAKSPGVRVNNECSMCGVKRVMLNGLRGEHTTILVDGVPTHSLV
                     SSVYGLDATSAAGVERIEIARGAGASLIAPEAIGGTINLITKQARADELDLDIAGGDN
                     NYRKIAAVGTLLANEDSTGITAIAQQDRRDQVDGDNNGVSENPLLDNKSLSLRISQDF
                     GSQDNVSLRAKHARSEIFGGPMATDIRAVKRDYFADPEFVSEHLFADDDVRHTFVAKP
                     WETAEWIESTRRELAASWLHEANSELNFTLTASSAQHQQDSFYEGFIYKADNDVAYFD
                     GRINYSLTDSQHLTFGLDSRRETLRSHTNSQSPAYVTDSFNYSSTGIYLQDTWTANTD
                     LEIAFALRADKLIADFIDPKKPADEIDQTLLSPRVDMRLRHSEAWNSRLSLGQGYRVP
                     LSFFESDHGLLDSDAGFNIAIDRVERSKSVNYSLNYDLGPISATSSIAYTQVENLAQL
                     TQDENDVPTLGQAQERADVTDYDIALTYKVNSQLSLSTVAETYHYNNAFKQAFSVAPI
                     EQRLSLSADFDWQGLDTYISAVWIGKRDLTDYATPVSPSFDLKGEQPKSTQAAGFWTL
                     DVQSSYEIGEGYKIYLGAYNLLNYTQVRDGQTPLFYQDGEFDVTHVYGPLRGREVYAG
                     VRFVF"
     gene            complement(2937..3179)
                     /locus_tag="PKZ52_14140"
     CDS             complement(2937..3179)
                     /locus_tag="PKZ52_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60960.1"
                     /translation="MSTQKPLPFTLTLLGSLLLAQLSHAHSVDEVVVTGVKERLYAAG
                     MLKDSIEKTEVVAAESMIRNRRTTSPKPLLNPPACG"
     gene            3306..>3548
                     /locus_tag="PKZ52_14145"
     CDS             3306..>3548
                     /locus_tag="PKZ52_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNG60961.1"
                     /translation="MLINHRKPVVEADSPRDSVHNYYERFVFEQLLRASDRAAADANY
                     FADVACVALNHLPPRYVRFDVDMSFFLSPQEMDEMAD"
BASE COUNT          848 a          906 c          911 g          883 t
ORIGIN      
        1 ataaaatgaa ctttagcctg catgcaattg ctcaacttaa tcgagagaat tacatcagcc
       61 tagttgtgac aaagtattaa tcaagccaaa ctttataatc ttttggcatg taccccaaaa
      121 gctccaaggg ctaataattg ttgttttgta ggggcttgtg gagggcttcc gccgggatgg
      181 aaaactgcag aggcacgcca cacaaggaga gactaaacgc tatctgcatg taggcgcttt
      241 tcaatttttt atttaattcc ccgcccgtta attctcctcc ttgagacaaa ataattaagt
      301 taagggaaaa gcccgttctg gcgtaggttg gaagagcgca gcgacttgtg acatcccgcc
      361 gagccctagt ggtgcctaga gtttatgctg gcggatggat gtcggaagtc gctgcgctct
      421 tccaacctac gagtaaaaat acgcctcaaa acgcttaatt taatgtggtt gctgctgccg
      481 cgcgatcgat tctgccgttt ggctaaatcc cacccaattt gccgcccgcc tggagagggt
      541 aataatgagt gcccaataat taccattaag acagagaatt aaggcactac ctgcgcccgg
      601 caaatcccat caagaatcgc gctttgcggt gcaaaacctt ttaattcagc cacttttacg
      661 ccctgcccat taaatataaa tgttaccggc gttgcgcgaa taggcccggt taattgtaat
      721 aaagcggaac tgctggcaaa ggcgggcagc agagttttac tgcggcgcaa ttcgttttgt
      781 aattgctgcc gcgtaccctg ggtgccgatt aatatcacct gcagttgatc gctgcagcgc
      841 tgctgcacat cattgagcat aagaatttgc cggtagcacc aggggcagtc cggctcaaaa
      901 aaactcacca caagtgattt gttgtgaaac tggctcaagg aggtcgtttc gggcgcctgc
      961 agggatttaa gcgcgataga aaatacattt ggcgttaatt ctgcagccca agcctgggcc
     1021 cccaccatca gcgcaagcgc agcacctata ttaaagacag cttttaacct ggtgctaaaa
     1081 aacaaaacgc acacccgcat aaacttctcg cccgcgcagc ggcccgtata catgggtcac
     1141 atcaaattca ccgtcttggt aaaataacgg cgtttgtccg tcgcgcactt gggtgtaatt
     1201 cagcagatta tatgcgccca agtatatttt atagccctcg ccaatttcgt aactactttg
     1261 tacatcaaga gtccaaaaac ctgccgcttg agtgctttta ggctgctcac cctttaaatc
     1321 aaagctgggg ctgacggggg ttgcatagtc ggttaaatcc cgcttgccga tccacacagc
     1381 gcttatataa gtatccaggc cctgccagtc aaaatctgcg ctgaggctga ggcgctgttc
     1441 gatgggggcc acactaaacg cctgtttaaa ggcattattg tagtgataag tttccgccac
     1501 tgtgctcagg cttaattggg aattaacttt gtaggtaagg gcaatatcat agtccgttac
     1561 atcggcgcgc tcttgtgctt ggcctagggt gggcacatcg ttttcatcct gggttaattg
     1621 cgccagattt tccacctggg tataggcaat agagctggta gcacttatgg gccccaagtc
     1681 gtaattaagc gaataattaa cagacttgga gcgttctacc ctatcgatgg caatattaaa
     1741 acctgcgtcg ctatctaata agccgtggtc gctttcaaaa aacgacaggg gcacgcgata
     1801 cccctggcct aaagaaagcc ttgaattcca ggcttcgctg tggcgcaagc gcatatccac
     1861 gcggggggat agcagcgttt ggtcaatttc atccgcaggt tttttagggt cgataaaatc
     1921 ggcaattaat ttatcagcgc gcagggcaaa ggcaatttcc aagtcggtat ttgccgtcca
     1981 agtgtcctgc aggtaaatgc ctgtagagct gtaattaaaa ctgtccgtta cataagccgg
     2041 gctttgacta ttggtgtggg agcgcagggt ttcgcggcga gaatccaggc caaaggtgag
     2101 gtgctgacta tcagttaggc tgtaattgat gcggccatca aaataagcta cgtcattatc
     2161 ggccttataa ataaatcctt cgtaaaatga atcctgctga tgttgagcgc tgcttgcggt
     2221 cagtgtgaaa tttaattcgc tattggcctc gtgcagccag ctagcggcta attcccgccg
     2281 cgtcgattca atccactcgg cggtttccca gggtttggcg acgaatgtat ggcgcacatc
     2341 gtcatcggca aataaatgtt cagagacaaa ctctgggtcg gcaaaataat cgcgttttac
     2401 cgcgcggata tccgtagcca tcggcccgcc aaaaatttcc gagcgggcgt gtttggcgcg
     2461 caggctgaca ttatcctggc tgccgaaatc ctgagaaatg cgcaggctta aggacttatt
     2521 atccaacagc ggattttcac tcaccccgtt gttgtcccca tcaacctgat cgcgccgatc
     2581 ctgttgggct atggcggtga tgccggtgct atcctcgttg gccaagagcg tgcccacggc
     2641 ggcgattttg cggtagttgt tgtcgccccc ggcaatatcc aggtccaatt catcggcgcg
     2701 ggcctgcttg gtaattaaat taatggtgcc gccaatggcc tccggggcaa ttaacgaggc
     2761 gcctgcaccg cgggcaattt caatgcgttc aactcctgcg gcactggtgg cgtccaggcc
     2821 gtagacgctg gacaccaaac tgtgggtggg caccccatcc actaaaatcg tcgtgtgctc
     2881 gccccgcagg ccgttgagca tcacccgctt tacgccgcac atggagcact cattgttcac
     2941 ccgcacgccg ggggatttag caatggcttg ggtgaggttg tccgcctgtt ccttatcata
     3001 ctctcggcgg ctaccacctc ggttttttca atgctgtcct taagcatacc ggcggcatag
     3061 aggcgctctt ttacgcccgt caccaccact tcatctacgg agtgggcgtg gcttaactgg
     3121 gcgagcaaaa ggctgccaag cagggtgagg gtaaagggca gaggcttttg ggtagacatg
     3181 gggcaaccgg gtacagcgaa cgaatgaaat gttatagtat ttcatttgtg gccagaagcc
     3241 aatgcccggc gcagggccca aaagccgact acacttctgg ggttcaccct gagcgagcct
     3301 gcgccatgtt aattaaccat cgtaaacccg ttgtagaagc cgactccccc cgcgatagcg
     3361 tgcacaacta ctacgagcgt tttgtgtttg aacagctgct gcgggccagc gacagagcgg
     3421 cagcggacgc caattatttt gccgatgtgg cttgcgtggc cctcaatcat ctgccgcccc
     3481 gttacgtgcg ttttgatgtg gatatgtcgt ttttcttatc cccccaagaa atggacgaaa
     3541 tggccgac
//
LOCUS       DAOQGS010000432         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15025_length_3547_cov_4.803837, whole genome shotgun sequence.
ACCESSION   DAOQGS010000432 DAOQGS010000000
VERSION     DAOQGS010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15025_length_3547_cov_4.803837"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKZ52_14150"
     CDS             <1..944
                     /locus_tag="PKZ52_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60962.1"
                     /translation="ARIEAGHLNLIYKTLSPCELVQRCLGMVQNLAAKRNISLVFYPG
                     NFASITITADQTRLEQVLLNLITNAVKYNRDRGRVNIACAIEADTYFRILVQDTGKGI
                     PVEKQSQIFQPFNRLGEENSKTEGSGVGLVITQRLVQQMGGRIDFSSQEGVGSRFWID
                     IPLRQEVVVSPPVVGQPVELPEPANNELEIGGRRRILYIEDSPSNQRLMEHLLSRFAQ
                     LELQLAGDALKGLFYARTEHPDLIIMDINLPGMDGFEALEVLQSDLATAHIPVIALSA
                     NAMSHDIEKGLKAGFVSYLTKPLEFGLLIEVFNRVLK"
     gene            1238..1393
                     /locus_tag="PKZ52_14155"
     CDS             1238..1393
                     /locus_tag="PKZ52_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60963.1"
                     /translation="MKIKNSFGAQSGIFLAVVCMRELLLAMQGGHIPALACHAFEFYI
                     LSLYSFI"
     gene            1546..2874
                     /locus_tag="PKZ52_14160"
     CDS             1546..2874
                     /locus_tag="PKZ52_14160"
                     /inference="COORDINATES: protein motif:HMM:NF040572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNG60964.1"
                     /translation="MTIEPGIEVVIQYQSGTWTADPHTNGGQLYDANGDLSVIVPENQ
                     TSYPIVGVAMGSLVGRVNGGTPFLIGAAAYAILSAIGGPLELCINDDLTGQYGAGLTD
                     NEGSLTIGVTVYANANTAPDLSQPLAREPAQQSPAVPPANLLGPLQYLIGTWTNKNLP
                     GTTKGGPDSPYSYNVMPLPQIDPSSPTGYILKNFSYYEELTFTAIHGNAPNRGGSGQQ
                     VAYTLFYEQRVYFAEGPNKDALVHAENGSLLYLLDTVQPLGPYGNGDQPGLGSLTVQN
                     SVAPTQQFNLVKQVSVPHGNSILALGNYASENQMTGVPIIPAASPLPSGVNTAQYAAN
                     DPVSNPQPALTANPNQVLTNALEARPCSNFLKLAMSSANGKGIVTNIGFEQQHANVSQ
                     YDFTYWLEAFDGTTDYTQLQYTQTITLQIPINGQLINFPHVTANTLTKSL"
     gene            3264..>3547
                     /locus_tag="PKZ52_14165"
     CDS             3264..>3547
                     /locus_tag="PKZ52_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNG60965.1"
                     /translation="MTYADHVKRLTPAEKRLVKYINEHWSRAQALDELKSHLQTAIEV
                     ELATIPIYLFAYYSINRTPAGFPDTALAQFADRAGASIMSVAVEEMLHMSL"
BASE COUNT          909 a          844 c          859 g          935 t
ORIGIN      
        1 tagcccgcat tgaagctggg catttaaacc ttatttacaa aactctgtcg ccctgtgaat
       61 tggtgcagcg ctgtttgggc atggtgcaaa acctagcggc gaagcgcaat atcagtttgg
      121 tgttttaccc gggtaatttt gcctcaatta ccatcaccgc cgatcaaacc cggttagaac
      181 aagtgttgtt gaatttaatt accaatgccg tgaaatacaa tcgtgatcgc gggcgggtga
      241 atatcgcctg tgcgattgag gccgatacgt attttcgcat cctggtgcag gatacgggca
      301 agggcattcc ggttgaaaaa cagagccaaa ttttccagcc gtttaatcgc ctaggcgaag
      361 aaaactctaa aaccgaaggc agcggtgtgg gcttggttat tacccagcgt ttagtgcagc
      421 aaatgggcgg gcgcattgat ttttccagcc aggagggcgt gggcagccgc ttttggattg
      481 atattcccct gcgccaagaa gtggtggtga gcccaccggt ggtggggcaa ccggtggagc
      541 tgccggagcc tgccaataat gaattggaaa ttggcggccg ccgccgtatt ttgtatatcg
      601 aagacagccc atccaaccag cgcctgatgg agcatctgct cagccgcttt gcgcagttgg
      661 aattacagtt ggcgggtgat gccctcaagg ggctttttta tgcccgcacc gagcacccag
      721 atttaattat tatggatatt aatttacccg gtatggatgg ctttgaagcg ctggaagtgt
      781 tgcagtcgga tctcgccacc gcccatattc cggtcattgc cttaagcgcc aatgccatga
      841 gccacgatat tgaaaagggc ttaaaggctg ggtttgtcag ttatttaacc aaaccattag
      901 agtttggttt gttaattgag gtgtttaacc gcgttttaaa ataatctttt ttcgtaattt
      961 ttggctgtaa acgaatttat caaaatccgc caaggcattg taagtgctta aaccgcacgg
     1021 atgcgcaatt tgctttgctg tattaacccg gcggcttttt atgccgggtt aataactgcg
     1081 gcaaggatgc cgcgccaaag gcgcaagccg ccgcgagctc cgctaaaaca agaaaatcca
     1141 cgcattttcg caagtgtcag cggagcgagg gcaaaataga tcaggacgat ctattttgcc
     1201 gccgggttaa taagacgaat tgccgccgca agcttttatg aagattaaaa actcattcgg
     1261 cgcgcaaagt ggcatttttc tggcagttgt ttgtatgcgt gagttattac ttgcaatgca
     1321 aggcggccat attccagctt tggcttgcca cgcatttgaa ttctatattt tgtcgctcta
     1381 ttcctttatt taatttgtgt cctaaaattc tctttctata ctcagcttct acgcgacaac
     1441 acagctctag aggcgatgat gaattcaacc actgcaatcc attctattct tggcgcactg
     1501 actaatataa ccctaaaggc gaatcaaccc tggcaatcta cgggtgtgac gattgagcct
     1561 gggattgaag tggtaattca gtatcaaagc ggtacctgga ccgctgatcc tcacaccaat
     1621 ggcggccaac tttatgatgc caatggtgac ctaagcgtca ttgtgccgga gaaccaaacg
     1681 agttatccga ttgtgggcgt ggccatgggg tctttggtgg gccgcgttaa tggtggcacg
     1741 ccatttttaa tcggcgctgc ggcctatgcg attttatcgg caattggagg gccgctggaa
     1801 ttatgcatca acgatgattt aacgggccaa tacggagcgg gtcttacgga taatgaggga
     1861 agcttaacta tcggggtaac cgtatatgca aacgccaata cggcaccgga tctttcccaa
     1921 cccttagccc gcgagcctgc gcaacaatcg ccagctgtac cgccggcgaa tttattgggg
     1981 ccattgcagt atttaattgg cacctggact aataaaaatc ttcctggaac caccaagggc
     2041 ggtccggatt caccttattc ctacaacgtg atgccgctgc cgcaaataga tccaagctcc
     2101 cccaccggtt atattctgaa aaactttagt tattacgaag aattgacctt tacagcgatt
     2161 cacggcaacg cacctaatcg cggcggcagc ggtcaacaag tggcttatac attattttac
     2221 gagcagcgcg tttattttgc tgaaggcccc aataaagatg cgctggtgca tgcggaaaat
     2281 ggctcgttat tatatctgct cgataccgtc caacccttgg ggccctatgg caatggtgat
     2341 caacctggcc taggttcgct taccgtacag aacagcgttg cacctacgca acaatttaat
     2401 ttggttaagc aggtgtcagt accccacggc aattcgattc tcgcgctggg caattacgcg
     2461 agtgaaaatc aaatgaccgg tgtacccatt attccagctg catccccttt gcccagtgga
     2521 gtcaataccg cccagtatgc cgccaatgat cctgtttcta atccgcagcc agcactcacg
     2581 gccaacccta atcaagtgtt aaccaacgca ttggaagcgc gcccttgcag caattttttg
     2641 aagttggcga tgagcagcgc aaacggtaag ggtattgtta ctaatattgg ttttgaacag
     2701 cagcatgcga atgtcagcca atatgatttt acctattggc tagaagcctt tgacggcacc
     2761 acagattaca cccaattgca gtatacccaa accatcaccc tgcagattcc gattaatggc
     2821 cagttgatta attttccgca tgtgacggcc aatacactca ccaaaagttt gtaattcggc
     2881 ggctttttat ttgctgtcgc caagttttta cacataaata actgttcagt aaggcttgtg
     2941 actctttccg cgtgcgctgc aaattgatag gggttttatc gattcgccac tgcgggttga
     3001 gtgttgaggg tgcgcaacaa taatacactc gcctgatatt ttttagtcgg tgcgggcatc
     3061 atgcccaact acactaaaag tgctaaatag ttacacccaa aaggctggta aatagttaca
     3121 acaatagaag cggtgattag ttacacccat agcgcacata tttatcgcct tgaacgcctc
     3181 cttgtacatt caatagagat tttttaagag aaagttccgt ataacaattt accctcagca
     3241 aaaacaagtg gaaggaataa agaatgactt atgccgatca tgtaaaacgc ttaacgcctg
     3301 ctgaaaagcg cctggtaaaa tatatcaacg agcattggag ccgcgcgcag gccctggatg
     3361 aactcaaatc acacctgcaa acggcgattg aggtggagtt ggcaactatt cctatatatc
     3421 tatttgccta ctattcaatt aaccgcacgc cggctgggtt tccggatacg gcgttggcac
     3481 aatttgccga ccgcgccggt gccagcatta tgagtgtggc tgtggaggaa atgctgcata
     3541 tgtcgct
//
LOCUS       DAOQGS010000433         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15035_length_3545_cov_4.745845, whole genome shotgun sequence.
ACCESSION   DAOQGS010000433 DAOQGS010000000
VERSION     DAOQGS010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15035_length_3545_cov_4.745845"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="PKZ52_14170"
     CDS             complement(<1..2267)
                     /locus_tag="PKZ52_14170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG60966.1"
                     /translation="MKSSDLHTLLTAQLAQLPAPTQPTAEYTALLELVSDTYHRQDRE
                     LAQREQTLKNIKYQLQSLLTIQQKLLSADEATSILNSICQIATQVISASRIYLWIYKE
                     NLFQPFYEMEYDQLLGRFTPVRLQACGLDQAYLDEVNYRQSVVLDDANNSPSLNPHSR
                     LYLSEQKIASLIDVPIATSCGYFGLLRLAQIYPGRHWSDSEQQFAVGLADKINQVAYK
                     YYCQHQKGIDESESRFKSLAQSTDAAIFAFRNFIFFSNSAMEKLTGFGQDAIRVLPVS
                     VLLGENFASQFNSHVLKNTDEGTKRRLEVEFTNRSGDTRWALVSVTATKLENRPTWLA
                     TAIDITERKRSEVQLNRQAFHDSLTGLPNRAKLLAEIDRNLVKVGRERHYKFALLFLD
                     LDGFKQINDNFGHLFADEVLIEIGLRLRNLADINHTAARLGGDEFAFLLTDTHSTEQV
                     QQFADKLLQEIHRPILNDDHGVHISASIGICLSDRFGETADLMLRHADVAMYQAKAEG
                     RNRCALFDRKLHAHTQRTLDVEKQLRARLRQGDFQFIFNPVLNTQSGVLAQFDCAINW
                     QLSNPESPAEPAQPNSDATLLMAIEDWSIQKAVQHHTTFNHPCLQLNIALSAHSLTDI
                     HFIRRLGRQLAILPAGAIACSVTESDWMLLHERYQKHLRLMEEIALHWIIEDFGSGHS
                     CLMYLAQLPVKALRLDARFMHTAMQAGGHQPIKALVDMGKNMGLPVIAKGVDTQQHLA
                     LARRLHCPLIQGAMV"
     gene            2573..3466
                     /locus_tag="PKZ52_14175"
     CDS             2573..3466
                     /locus_tag="PKZ52_14175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNG60967.1"
                     /translation="MDEPTGRFDNKMDVYRAGFMDGVGVELITAINKTIDLVATVVIP
                     FEILVLIITRRTLIQREIMVNLACALAILATLHTVDFSYKYASLEYVRAHAFFSLHNA
                     NSPGVLTLVILAHVLVGDLCFYVSHRLRHTKYLFMLEHVVHHSSTELNFVTNLRTSFV
                     VTFYGWLPLLVPLLFGFDPALLLACFALANALPFFMHHNLIKKMGWLEYVFNTPSHHR
                     VHHGSNPCYIDKNFGGVFIIWDRLFGTYADEVEPVVYGLSCGKPSGNPFNVLFGGWVR
                     LFKIIKRKLGKNSLPAHRQAE"
BASE COUNT          826 a          790 c          939 g          990 t
ORIGIN      
        1 tttaccattg ccccttgaat taaggggcaa tgtaaacgcc gggccagtgc caagtgctgc
       61 tgggtatcca cgcccttggc gataactggc agccccatgt ttttgcccat gtccactagg
      121 gctttaatcg gttggtggcc gccagcttgc attgccgtgt gcataaagcg cgcatccagt
      181 ctaagtgctt ttaccggcag ctgagccaaa tacatcaggc aggaatggcc gcttccaaaa
      241 tcctcgatga tccagtgcag ggcaatttct tccatcaggc gcaggtgttt ttgatagcgc
      301 tcgtgcaaca gcatccagtc gctttcggtg accgagcagg cgatagcgcc ggcgggcaga
      361 atggctaatt ggcggcccag cctgcgaata aaatgaatat ccgtgaggga gtgggcgcta
      421 agggcaatat ttaattgcag gcaggggtga ttaaatgtgg tgtggtgctg cacggctttt
      481 tgaatggacc agtcttcaat ggccattaat aaagtggcat cgctgttggg ctgcgcaggc
      541 tctgcgggcg actccggatt ggataattgc cagttaatcg cacagtcaaa ctgggctaaa
      601 acccccgact gggtattcag cactgggtta aaaataaatt ggaaatcccc ttggcgcagg
      661 cgcgcgcgca attgtttttc gacatccaag gtgcgctggg tgtgcgcgtg taatttgcgg
      721 tcgaacaatg cgcagcggtt gcgcccttcg gccttggctt ggtacatggc cacatccgca
      781 tggcgcagca ttaaatcggc ggtttcacca aaacggtcac ttaaacaaat gccgatgcta
      841 gcgctgatgt gtacaccgtg gtcgtcattg agaatcgggc ggtgaatttc ttgtagcaat
      901 ttatcggcga attgctgcac ttgctctgtg gagtgtgtat ccgtaagcaa aaatgcaaat
      961 tcatcgccgc cgaggcgcgc tgccgtgtgg ttgatgtccg ccaggttgcg caggcgcagg
     1021 ccaatttcaa ttaatacttc atcggcaaat aaatggccga aattgtcgtt aatttgttta
     1081 aaaccgtcta aatccaaaaa taacagtgca aatttatagt gccgttcgcg gccaactttt
     1141 accaggttgc ggtcaatttc cgccagcagc tttgcccggt tgggaaggcc cgtgagggag
     1201 tcgtgaaagg cttggcggtt aagctgtacc tccgagcgtt tgcgctcggt gatatcaata
     1261 gcagtggcaa gccaggtggg gcggttttct aatttggtgg ctgtgacgct tactagcgcc
     1321 cagcgggtat cgccgctgcg gttggtaaat tcaacttcca ggcggcgttt ggtaccctca
     1381 tcagtatttt ttaatacgtg ggaattaaac tggctggcaa aattctcgcc caacaataca
     1441 ctcaccggca agactcgaat ggcatcttgg ccaaaaccgg tgagtttttc catggcgctg
     1501 ttgctaaaaa aaataaaatt gcgaaacgca aatatggccg catcggtaga ttgggcgagg
     1561 gatttaaagc gcgactcgct ctcatcaatg cccttttggt gctggcaata atatttatag
     1621 gccacctggt taattttatc ggcaagcccc accgcaaatt gttgttcact atcactccag
     1681 tgcctgcctg ggtatatttg cgctaggcgc aacaggccaa aatatccgca gctagtagca
     1741 atcggcacat caattaagga ggcaattttt tgttcgctta aatacaagcg gctgtgcggg
     1801 ttcagtgagg gtgaattatt cgcatcatcc agcaccaccg actggcggta attgacttca
     1861 tctaggtacg cttgatcaag gccgcaggct tgtaaacgca ccggtgtaaa acggcctaag
     1921 agttgatcgt attccatttc ataaaagggc tgaaacaagt tctctttata aatccacaga
     1981 tatatacggc tggcgctgat gacttgtgtg gcaatttggc aaatcgaatt taaaatactg
     2041 gtcgcttcat ctgcgctgag gagtttttgt tgaatggtca acaaactttg cagctgatat
     2101 ttaatatttt taagggtttg ttcccgctgg gctaattcgc gatcttgccg atgataggtg
     2161 tcggacacta gctccagtaa tgctgtgtac tccgccgttg gttgcgtcgg cgctggcagc
     2221 tgggctaatt gcgctgttaa taatgtatgt aaatctgaac ttttcacagg cagaggcgat
     2281 gggttagtgg atgggatatc aattcttaat ggtaaggcag aaggcggtgc agtataaccc
     2341 agagcccgtg cttgggaagc ttcaatagtt gcttttaggg ctgtgtgcaa cttgccttct
     2401 ggggtaactc ttcagctgag gtttttaggt tcaagggata tgggcccatg ccaatgaagt
     2461 ttctccctta agtgtggctt ttgaccctgc tggtgcgcct gtgtcggccc aggtgcggcg
     2521 gtaaataggc cggaagcagg tcacatttgg tgcgaaatac gtaaatttac gtatggatga
     2581 gcctactggc aggttcgata ataaaatgga cgtgtatcgg gcagggttta tggacggcgt
     2641 aggcgtagag ctgataacag caattaataa aactattgat ttggtcgcta ccgtggtgat
     2701 tccgtttgag attcttgtgt taattatcac ccgccgcacg cttatacagc gggaaattat
     2761 ggtaaatctt gcctgtgccc tcgccatatt ggccacctta catactgtgg acttcagtta
     2821 taagtacgca agcctagagt atgtgcgcgc ccacgcgttt ttttcgttgc acaatgctaa
     2881 ttcccccggc gtattgacct tggtcatttt agcccatgtg ttagtgggcg atttgtgttt
     2941 ttatgtgagc caccgcctgc gccacaccaa gtatttattt atgttggagc atgtggtgca
     3001 ccattcgtca accgaattaa atttcgtcac caatttgcgc actagttttg tggtaacttt
     3061 ttatggctgg ctgccgttgc tggtgccgct gttatttggc tttgatccag cactcttatt
     3121 agcctgtttt gccctggcca atgcattacc gttttttatg caccacaatt tgattaaaaa
     3181 aatgggttgg cttgaatacg tatttaatac accctcccat caccgggtgc accacggttc
     3241 taacccgtgt tatatcgata aaaattttgg cggtgtattt ataatctggg atagattatt
     3301 tggcacctac gccgatgagg tggagcctgt ggtctatgga ttaagctgcg gcaaacccag
     3361 cggcaatccg tttaatgtgt tgttcggcgg ctgggtgcgg ttgtttaaaa tcattaaacg
     3421 caaacttggc aaaaacagtt tgccggccca caggcaggct gagtaataat tgtaactatt
     3481 cagcatcttt ttacgtgagt tgccaataat ttataggacg ggctgattaa gtttgaggcg
     3541 gggat
//
LOCUS       DAOQGS010000434         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15070_length_3540_cov_4.739742, whole genome shotgun sequence.
ACCESSION   DAOQGS010000434 DAOQGS010000000
VERSION     DAOQGS010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15070_length_3540_cov_4.739742"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3540)
                     /locus_tag="PKZ52_14180"
     CDS             complement(<1..>3540)
                     /locus_tag="PKZ52_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNG60968.1"
                     /translation="DEQTYAKAKPHFEAVWKDFQAAGRDLADFVRAIRDNFGAGVKPY
                     LSRFAKEKREELLNAANPNNERDSRNRTDEVEATERSVQPEKTGARGRGAGQPDGQPG
                     NQGFDFGDSVPSVRPAANGERSGGRSEAQQSSVGARAAGAIDDRGGPDDGAARLYAER
                     APDGAIEGATEQAAAVGETSLKPVPGEIKQASASQIKARMPFLTDGQVEDVVFAEKRL
                     AKTDGFGVMFTNGTGTGKTFTGLGIVRRMLDAGKKNVLIVAPKQTILDGWKKAASGFF
                     GIDASILPDTKSNGGGGVVGTTYAALASNNKLSEIKWDAIVADESHCLSSDEAGNKTE
                     AQKSFDALAGNNARRLASMLRPDLYERAADLRSKISSYNKSDNQQAWAEAAKLEDSLD
                     AVVREIGAHERAVIESVEAVRPQDKARVVFLSATPFAYDKNIQYANGYLFDWGSDKDG
                     LRYNSGGNREQFFMQHFGYSMRYNRLTAPDARVDRGLMERNFNATLKAQGVLSGRALD
                     SDFDYDRRFVLAESKIGKRVDDAIKWLNEAASGKAAIAGGDTLRDEILKDKFDYHARM
                     YFLEAIKAREALPYIESQLGAGRNVLVMHDFKKGGTLNPFSQRINNPDSALQSAYAAF
                     KKEFKDLIGAFEALPSPIDLLSDKYPGALIYNGNVSARERIKLQDMFNDDTRPPMVMI
                     AQGDAMREGVSIHDTTGKRQRVLIHLGMPVKPTAAIQQEGRIYRTGQKSNALFRYFTI
                     GTAWERMAFASKIAGRAGTAENLAMGEEARGLKQSFIDAYEDAQDWPVGYDGEGVGGK
                     AEDRARAQNLTRWDQAKAYYFGTKKQGKGQSSAGREGNDYFATPEPVGLKMVEWSAAA
                     PGDRVLEPSAGHGAISRWFPEGAKVRAIEQSADLASRLTLHTDGDVINGNFEDHHIVN
                     KYDAIVMNPPYGVGGSTAIAHLAKAAQHLAEGGRVVALIPTGPAADKKFDAWLFGKDA
                     KGKNNEPDLHLVMDVSLPSSTFERAGTSVSTRIVVIEKVSIGSEHVTKIRPSKNISLN
                     KDLSIEELFDSIENISAPPKATPIQEEVEAPKKGKSTEVGEVSGNKIITDAPEVEYTT
                     KKGKLLKGVLLDMPLDKAREIDKYSWVTTINGVKGAFVRMEYIQRPESMFSLGDTVAT
                     PISQSEVAEVINRVQSNFGAKLKITAVASAVEL"
BASE COUNT          747 a         1022 c          800 g          971 t
ORIGIN      
        1 ggagctcaac cgcgctcgca actgcagtta ttttgagttt cgcgccaaag ttggattgca
       61 ctcgattaat aacttcagcc acttcacttt ggctgatggg tgtagcaacc gtatcgccta
      121 gagaaaacat tgattccgga cgctgaatat attccatcct tacgaacgcg ccctttactc
      181 cgtttattgt tgttacccag gagtacttat caatttcgcg cgccttgtcc aggggcatat
      241 ccaaaagcac accctttaac agctttcctt ttttcgtggt gtattcaacc tccggggcgt
      301 ctgtaattat tttatttccg gacacctcac cgacttcagt gcttttgcct tttttcggcg
      361 cctccacctc ttcctgtatc ggcgttgcct ttggtggcgc acttatgttc tcaatgctgt
      421 cgaaaagctc ttcgatgctc aagtccttgt taagcgatat gtttttagat gggcgaattt
      481 ttgtaacgtg ttcagaccca atcgacacct tttctataac gacaattcgg gttgatacgc
      541 ttgttcctgc ccgctcaaat gttgaggacg gcaacgaaac atccatcacc aaatgcaggt
      601 cgggttcgtt atttttcccc tttgcatctt ttccaaatag ccaggcatca aatttcttat
      661 ctgcagcggg tccagtcgga attagagcta caacacggcc cccttcggcc aaatgctgtg
      721 ccgccttggc aaggtgcgca atggccgtac taccacccac cccataaggt ggattcatca
      781 caatggcgtc atatttattg acgatatggt ggtcttcaaa atttccgtta attacatcgc
      841 catcggtatg cagggttaag cggcttgcca aatcggcgct ttgctcaatg gcgcgcacct
      901 tggcgccctc cgggaaccat cgtgatatgg caccatggcc agcgctaggc tctagaactc
      961 tgtcaccagg cgccgcagca gaccactcca ccattttcaa tcctaccggc tcaggcgtgg
     1021 caaaataatc attaccctct cgccctgcag atgattggcc cttgccctgc ttttttgtgc
     1081 cgaaatagta ggccttcgcc tgatcccatc gggttaaatt ttgcgccctt gccctgtctt
     1141 ctgctttgcc gccaacgccc tcaccgtcat agcctaccgg ccagtcttga gcgtcctcgt
     1201 aggcgtcaat aaacgattgc ttaagccctc gcgcctcctc tcccatggca aggttttctg
     1261 ccgtgccggc gcgccctgcg atttttgagg caaacgccat gcgctcccac gccgtgccaa
     1321 tcgtgaagta ccggaacagc gcattgctct tttgtcccgt gcggtaaatg cgcccctctt
     1381 gctgaattgc ggcagttggt ttaactggca tcccaaggtg aatcaatacc cgctgtcttt
     1441 ttcctgtggt gtcgtgaatg cttacgcctt ctcgcattgc gtcgccctga gcgatcatca
     1501 ccataggggg cctggtgtca tcattaaaca tatcctgaag ctttatgcgc tcccttgcgc
     1561 tcacattccc gttataaatt aaagcaccag ggtatttgtc acttaaaaga tcaattgggc
     1621 ttggcaaagc ctcaaacgcg ccaattaaat ccttaaactc tttcttgaat gcggcataag
     1681 cagattgcag agctgaatct ggattgttaa tgcgctgaga gaatggattt agcgttccgc
     1741 cctttttaaa atcgtgcatg accaatacat ttctgccggc ccctaactga gattcgatgt
     1801 agggcagcgc ctcgcgagcc tttatggcct ccaaaaagta catgcgggcg tgataatcaa
     1861 acttatcctt caatatttcg tcgcgcagcg tgtcaccgcc agcaatggct gctttgcctg
     1921 acgccgcttc gttaagccac ttaatggcat catccactcg cttaccaatt ttgctttcgg
     1981 caagcacaaa gcggcgatca taatcaaaat cagaatcaag ggcccgccca gacaaaacgc
     2041 cctgagcctt gagcgtcgca ttgaaattgc gttccattaa ccctctatca actctggcgt
     2101 ctggggcggt aagtcggtta tagcgcatgg agtaaccgaa gtgctgcata aaaaattgct
     2161 ctcgattacc tccagagtta tacctcaatc catctttatc gctgccccag tcgaacaaat
     2221 agccgtttgc atactggatg tttttatcat aagcaaacgg ggtggcggat aagaaaacca
     2281 ccctagcttt atcctgtggt cttaccgcct ccaccgactc tataaccgct cgctcatgcg
     2341 cgccaatctc ccttaccacc gcatccaaac tgtcttcaag ttttgcagcc tctgcccacg
     2401 cctgctggtt atcactttta ttgtatgagc ttattttgct gcgcagatcg gcggcgcgct
     2461 cgtataagtc cgggcgcaac atggaggcaa gccggcgggc attattcccg gccagagcat
     2521 cgaaggattt ttgggcctcc gttttattcc cggcctcgtc gctagacagg caatgggact
     2581 catctgcaac gattgcatcc cacttgattt cgcttagctt gttattgctg gccagcgcag
     2641 cgtaagtggt cccgacaact ccaccgccgc cgttggattt tgtgtccgga agaatggagg
     2701 cgtcaattcc aaaaaaacct gaggcggcct tcttccagcc gtcgagaatg gtttgcttgg
     2761 gggcgacaat aagcacattc tttttgcccg cgtcgagcat tctccgaacg atgcctagac
     2821 cggtaaatgt ttttcccgtg cccgtgccat tggtaaacat gacaccgaag ccatcggttt
     2881 tcgcaagacg cttttcagca aatacaacat cctcaacctg tccgtcagtc agaaatggca
     2941 ttctggcttt tatctggctg gcacttgcct gctttatttc tccgggtaca ggcttaaggc
     3001 ttgtctcgcc tactgcagcg gcttgctcag tagcgccttc aattgctccg tctggagctc
     3061 gctcagcata tagtcgcgct gcgccatcgt cagggcctcc cctgtcgtca atagctccgg
     3121 cagcacgtgc gcccactgag gattgttgcg cctcacttct tccaccgctc cgctctccat
     3181 tagcagcggg gcgtacactt ggaacgctat cgccaaagtc gaatccttga tttccaggct
     3241 gtccgtcagg ctgtccagcg cctcgtccgc gcgctcctgt tttttcaggc tgaacactgc
     3301 gctcagtcgc ttcgacctca tcagtccggt ttcggctatc gcgttcatta tttggattgg
     3361 cagcatttag cagctcctct cgcttctctt tagcaaatct tgataaatac ggtttaactc
     3421 cggcgccaaa attatcgcga atcgcccgca caaaatcggc caggtcgcgc cctgctgcct
     3481 gaaaatcttt ccaaacagcc tcaaagtggg gttttgcctt ggcataggtt tgttcgtcaa
//
LOCUS       DAOQGS010000435         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15071_length_3540_cov_4.526255, whole genome shotgun sequence.
ACCESSION   DAOQGS010000435 DAOQGS010000000
VERSION     DAOQGS010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15071_length_3540_cov_4.526255"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKZ52_14185"
     CDS             <1..1206
                     /locus_tag="PKZ52_14185"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNG60969.1"
                     /translation="PISPALAPPSFAVAPTERTLSYRYTQYNEMSIDPKKVSSINADE
                     GGGSNQRYDIDINQYAFSTPIGDHFSMSVDIQDEVLTGASPWTVTKEDVKGTPSDTSD
                     DEIKLVMSGATIKEHRTEANVATTYYYSTGTISGNVGFSTEDDYESTSAGLSWGGEFD
                     DKQTGVSVGVSYSDDSVDPEEGSYKGTELGGKIGGWLNGSDPVVGTAKKESVSGFISG
                     SRILSPNWIVQAGVSYTQKSGHLSDPYKANDKRPDERNLYTFNAGSRYWIKPIKAALH
                     ADYRYYSDDWGIESHTLSLALYQNWQKLQIIPSLRYYTQTSASFYSISDPETTLYNYY
                     ADDHRLSNFGAITTGLKIVFKQKPVDWIVSGEYYVSGADMALDKADEPEHPGLLKYAK
                     FTFGVEHKF"
     gene            1259..2113
                     /locus_tag="PKZ52_14190"
     CDS             1259..2113
                     /locus_tag="PKZ52_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG60970.1"
                     /translation="MGSPCQIRLRAPSLSHAQALCAPLIQEALRFEAKYSRYLPTSLV
                     SHINNSAGATQILDAEAAAIVDYAAQLYQQSEGLFDITSGLLRQAWDFSSAQVPSQHR
                     IQALLPHIGWLHVSWQNPQLCLPAGMQIDLGGIVKEYAADALAQCAQAQGINSGLIEL
                     GGDIRVFGESTWPIGISDPQQPGRPLLQLKLQAGGFATSGNYQRYFIYQGKRYSHILN
                     PKTGWPVVTPAGVSVIADNCLIAGSVSTLALLMGEEQCLDWLYDVGLPFLCVFNDGRV
                     VNEFSQTG"
     gene            complement(2137..2619)
                     /locus_tag="PKZ52_14195"
     CDS             complement(2137..2619)
                     /locus_tag="PKZ52_14195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNG60971.1"
                     /translation="MTETLSIPLQTIDFKSTSLAEFPARAYLIVNTASKCGFTPQYTG
                     LEQLWQTYRGRGLQVLGFPCDQFGGQEPGSEAEISDFCQLNFGVSFPLFAKIDVNGAN
                     THPLFAALKKDAPGILGTEAIKWNFTKFLLDGSGTVIKRYAPATKPEEITPDIERLLA
                     "
     gene            2839..3387
                     /locus_tag="PKZ52_14200"
     CDS             2839..3387
                     /locus_tag="PKZ52_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60972.1"
                     /translation="MAILTQDMANFLEEWSSLGGPTARKYNLPVSVMLACASVESNWG
                     KGKIYAGSLNPFSLQKWPHTPKPSAYKTYWSETVVQTDPKVVRKAPFACAMSREDAVR
                     QWCEWILYYGEADGPPGNQNPKATRQANKAAIERRVKLLALRNNPEEFAGHLYWVGFG
                     EEKYKDLYKQRLKDYSMRKFDA"
BASE COUNT          903 a          855 c          909 g          873 t
ORIGIN      
        1 cctatttcac cggctttggc gccgccaagc tttgcggtgg cgcccaccga gcgcacgctg
       61 agctaccgct acacccaata caacgagatg agcatcgacc cgaaaaaagt cagcagcatt
      121 aatgctgatg aaggcggcgg ctctaatcag cgctacgata tcgacatcaa ccaatacgcc
      181 ttcagcacgc ccattggcga tcatttctcc atgtcggtgg atattcaaga tgaggtgctc
      241 accggcgcct caccttggac ggtaaccaaa gaagatgtta aaggcacccc ttcggatacc
      301 agtgacgacg aaattaaact ggtcatgagc ggcgcgacga ttaaagagca ccgcaccgag
      361 gccaatgtgg ccactaccta ttactacagc accggcacta tcagcggcaa tgtggggttc
      421 tccacggagg acgattacga atccaccagc gcaggcctga gctggggcgg cgaatttgac
      481 gataaacaaa ccggtgtgtc tgttggtgtg agttattcag atgacagcgt cgatccagaa
      541 gagggcagct ataagggtac tgaactgggg ggcaaaatcg gcggctggtt aaatggttca
      601 gacccagtgg tgggcacggc taaaaaagaa agtgtgtccg gttttatcag cggttcacgt
      661 attctttccc ccaactggat tgtacaagcg ggcgtgagtt acacgcaaaa atccgggcat
      721 ttaagtgacc cctacaaggc caacgacaaa cgcccagatg agcgcaattt gtataccttt
      781 aatgccggtt cccgctattg gattaaaccc attaaagcgg ccctgcacgc cgactaccgc
      841 tactacagcg acgactgggg tattgaatcc cacacattga gtttggccct ttatcagaat
      901 tggcaaaaac tgcagattat tccctccctg cggtattaca cccagacttc ggcgtctttt
      961 tattccatct ccgatcctga aaccacgctc tataactact acgccgatga tcaccgccta
     1021 tcgaatttcg gcgcgattac caccggttta aaaatagtgt tcaaacaaaa acctgtggat
     1081 tggattgtct ctggtgagta ctacgtgtct ggcgctgata tggcgctgga taaagccgat
     1141 gaacctgagc accccggctt attaaaatac gccaaattta cctttggcgt tgagcacaag
     1201 ttttaagcct taatcggcac cctctgtgga gagcctatat tccgttgcct ttcgggctat
     1261 gggctctccc tgtcaaatcc gcctgcgggc gccatctctg tcccacgcgc aagcactctg
     1321 tgcaccattg attcaagaag ccctgcgctt tgaagctaaa tacagccgtt atttacccac
     1381 tagtttggta agccacatta ataattcggc aggcgcaacc caaatactag atgctgaagc
     1441 ggccgccatt gttgattatg ccgcacagct gtatcagcag agtgaaggat tattcgatat
     1501 caccagcggg ctgctgcgcc aagcctggga tttttccagc gcccaggtgc cctcccagca
     1561 caggatacag gccctattgc cgcatatcgg ttggctgcac gtaagttggc aaaaccctca
     1621 attgtgttta cccgccggga tgcaaattga tttgggcggt attgttaaag aatacgcggc
     1681 agatgctctg gcccagtgcg cccaggcgca gggaattaac agcggtttaa ttgaattagg
     1741 cggcgatata cgcgtgtttg gcgagagcac ttggcccatt ggtattagcg atccacagca
     1801 gccgggcagg ccgctgctgc aattaaaact acaggcgggt ggctttgcca ccagcggcaa
     1861 ttatcagcgc tattttattt atcaaggtaa gcgttacagc catattctca accccaaaac
     1921 cggctggcca gtggtaacac ccgctggtgt atcggtaatt gccgataatt gtttaattgc
     1981 cggcagcgta tccaccctgg cactgctcat gggggaagag cagtgtttag attggttata
     2041 tgatgtgggt ttgccgtttt tgtgtgtatt taatgatggc cgcgtggtga atgagttttc
     2101 ccaaactgga taaaattgtt tgggatcaaa atacttttaa gctaataagc gttcaatatc
     2161 gggcgtaatt tcttcaggtt tggtggccgg tgcgtagcgc ttaataaccg tgccgctgcc
     2221 gtcgagcaaa aacttcgtaa aattccattt aattgcttca gtgccgagaa tgccgggggc
     2281 gtcttttttt aaagcggcaa aaagtggatg tgtgttggcg ccattgacgt caatcttggc
     2341 gaataacgga aaactcaccc caaaatttaa ttggcaaaag tcgctaattt ccgcttcgct
     2401 gccgggctct tgtccgccaa attgatcaca ggggaagccc aacacctgta aaccgcgccc
     2461 ccgataggtt tgccataact gctctaaccc cgtgtattgg ggtgtaaaac cacatttgct
     2521 ggcggtattt acaatcaggt aggcacgggc gggaaactcc gccaggctgg tggatttaaa
     2581 atcgatggtc tgcagtggga tggataaagt ctcggtcatg agggctcgct cataaggttc
     2641 tgtgaaggcg ggtgattgct ttcttggtga aagcaaagcg gcggtaaaac ttgggtttta
     2701 gtcaggttga ttgcgtgcgt acgtaaaacg cgcggtgttg gatcaggccg tgttcgcgaa
     2761 tataaattgt caactggcct aaatcaatgt acaagatgag cgcgttaata aaatttaacc
     2821 ctacctcgcg aggtgattat ggctatctta actcaggata tggcaaattt tttagaggag
     2881 tggagcagtt taggcgggcc tacggcgcgc aaatataatt taccggtgag tgtaatgttg
     2941 gcctgtgcct cggtggaatc aaactggggg aaagggaaaa tttacgccgg ttcgttaaac
     3001 ccatttagtt tgcaaaaatg gccccataca cccaaaccca gcgcctataa aacctattgg
     3061 tcggaaactg tggtgcaaac cgaccccaaa gtcgtacgca aagcgccgtt tgcctgcgcc
     3121 atgtccagag aggacgccgt gcgccaatgg tgtgagtgga ttttatatta cggcgaagcc
     3181 gacggcccac cgggcaacca aaaccccaaa gcaacacgtc aagccaacaa ggcggcgatt
     3241 gaacgacggg taaaactgtt ggccttgcgc aataatccag aggaatttgc cgggcatttg
     3301 tattgggttg gttttggtga ggaaaaatat aaggatttat ataagcagcg tttaaaggat
     3361 tactcaatgc gtaaatttga tgcctaaact cacctcaata taatgagcgg ttgcaggtaa
     3421 ttgcagattt taattatttg taactattca gcacacttta cttccctctt ctgcgcagac
     3481 ggcatattca ggaggattta gccatccggc ataatcgatt cgcggtgtag cagtggtgac
//
LOCUS       DAOQGS010000436         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15087_length_3539_cov_3.667623, whole genome shotgun sequence.
ACCESSION   DAOQGS010000436 DAOQGS010000000
VERSION     DAOQGS010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15087_length_3539_cov_3.667623"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKZ52_14205"
     CDS             <1..636
                     /locus_tag="PKZ52_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG60973.1"
                     /translation="MAGNGAQALTFAVQNRYDLFLFDLQMPFVGGKEAAEILIRQGIK
                     TPIIAFTANVMTHQRAEYESIGFADILEKPFNRWQVATLLQKYLPKLELRVNRVLVVD
                     DDPVNQMLARQLMLSQRPGITVHEALTAEIGLELIERGERYDLILMDLHLPGMNGFAA
                     TEELRKRNITSPVYILSANSEKDHQEAVVHCDAQGFIEKPLNRARLKQLLV"
     gene            833..2650
                     /gene="typA"
                     /locus_tag="PKZ52_14210"
     CDS             833..2650
                     /gene="typA"
                     /locus_tag="PKZ52_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNG60974.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLSQSGTLDRKDQGAERIMD
                     SNDQERERGITILAKNTAITWNGYRINIVDTPGHADFGGEVERVLSMVDCVCLLVDAV
                     DGPMPQTRFVTSKAFEQGLNPIVVINKVDRPGARPDWVVDQVFDLFDRLGATDEQLDF
                     PIIYASALNGVAGLSPDELASDMTPLFQMIVDKVPPPKVDIDGPLQMQISALDYNSYV
                     GIIGIGRITRGKIGPNKQVVVADLHGNKRKAKVLQVMGYHGLERIETQEASAGDIVCI
                     TGIDKLGISDTICDPDCVEILPPLTVDEPTVSMTFQVNDSPFAGLEGKYVTSRNIKDR
                     LEQELIHNVALRVTPGESPDKFKVSGRGELHLSVLIENMRREGFELGVSRPEVVTREV
                     DGEIHEPYEQVVIDVEEQHQGSVMEELGLRRGELTNMVPDGKGRVRLEFIIPSRGLIG
                     FRGSFLTMTSGSGIMTSIFDHYGPVKGGNMAKRQNGVLVSMVRGKTLAYALFALQERG
                     RLFLGHAEEVYEGQIVGLHSRDNDLVVNPTKAKQLTNIRAAGTDEALVLTPHIRHTLE
                     QALEFIEDDELVEVTPKSIRIRKKLLTENERKRASKSGS"
     gene            complement(2710..3261)
                     /locus_tag="PKZ52_14215"
     CDS             complement(2710..3261)
                     /locus_tag="PKZ52_14215"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF006605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNG60975.1"
                     /translation="MSQTSTDGLGEFSEQLYSFEQVSSAIAALAQDINSRLPGCEPLL
                     VLCLLNGGLVFTGQLLPHLKRPVELSYVHATRYHNNVAGAELRWISPQHADIQGKTIL
                     LCDDIFDEGKTLHAVYQELLHQGASSVHCAVMTTKDHPRKPANFRPEFSALWVPDRYV
                     FGMGMDYSGLWRNAPGIYAVHGM"
BASE COUNT          899 a          892 c          903 g          845 t
ORIGIN      
        1 atggcaggca acggcgccca agcgctgacc ttcgcggtgc aaaatcgcta cgacctattt
       61 ttattcgatt tacaaatgcc ttttgtgggc ggcaaagaag cggcggaaat tcttattcgg
      121 cagggtatca aaacacccat tatcgccttt actgccaatg taatgaccca ccagcgggcc
      181 gaatatgaaa gcatcggctt tgccgatatt ttagaaaaac cgtttaaccg ctggcaggtt
      241 gccaccttgc tgcaaaaata tttacccaag cttgagctga gggtaaatcg tgtattggtg
      301 gtggatgatg acccggtcaa tcaaatgttg gcgcggcaat taatgctctc ccagcggccg
      361 ggtattacgg tgcatgaggc gcttactgcg gaaataggtt tggaattaat cgagcgcggt
      421 gagcgttatg atttaatttt aatggattta cacttgccgg gtatgaatgg ttttgccgcc
      481 accgaagaat tgcgcaagcg caatatcacc agccccgttt atatattaag tgccaattct
      541 gaaaaagatc atcaagaggc cgttgtgcac tgcgacgcgc agggatttat tgaaaaaccc
      601 ttaaaccgcg cccgcttgaa gcagttgttg gtgtgacctg ccggcgcatc tttttgtggc
      661 acagcatgtc gcctgcccgc taatttcaca cgccgccgcc acttcctgtg cagcctatta
      721 aggattattc gcagtattgc tatctgctgt catattaagt gtctataatg ccgcccttct
      781 tttagtacca cagcctcccg tgctgttgtg attgttccct gaggatccat ttgtgataga
      841 aaaattgcgc aatattgcca ttattgccca cgtagaccat ggcaaaacca cccttgttga
      901 taagctgctg tcgcagtctg gcaccttaga tcgcaaggac caaggcgctg agcgcatcat
      961 ggattccaac gaccaagagc gcgagcgggg cattaccatc ctggcgaaaa acaccgccat
     1021 tacctggaac ggctaccgca tcaacatagt agacacccca ggccacgccg acttcggcgg
     1081 cgaagtagag cgcgtactgt ccatggtgga ttgtgtgtgc ctgttggtag acgcagtaga
     1141 cggccctatg ccacaaaccc gctttgtaac ctccaaagcc tttgaacagg gcttaaaccc
     1201 catagtagta atcaacaaag tagaccgccc cggcgcccgc cctgattggg tggtggatca
     1261 agtgtttgat ctcttcgacc gccttggcgc caccgacgag cagttggact tcccgattat
     1321 ttacgcctcc gccttaaatg gcgtggccgg tctgtcaccg gatgagctcg cctccgatat
     1381 gacgccttta ttccagatga tcgtggataa ggtgccacca ccgaaagtag atatcgacgg
     1441 ccctttgcaa atgcaaatct ccgccctgga ttacaactcc tacgtgggca ttatcggcat
     1501 tggccgtatc acccgcggca aaatcggccc caacaaacag gttgtagtgg cggacttaca
     1561 cggcaacaaa cgcaaggcca aagtattgca ggtaatgggt tatcacggcc tggagcgcat
     1621 cgaaacccag gaggcctccg caggggacat agtctgtatc accggtatcg ataaattagg
     1681 tatttccgac accatttgtg atcccgattg tgtggaaatt ctgccgcccc tcaccgtgga
     1741 tgagcccacc gtgagcatga cgttccaggt gaatgactcg ccttttgccg gtttagaagg
     1801 caaatacgtt acctctcgca atattaaaga ccgcttggaa caagagttaa tccataacgt
     1861 ggccctgcgg gtaactccag gtgaaagccc cgataaattt aaagtatccg gtcgcggcga
     1921 attacaccta tcggtattaa ttgaaaacat gcgccgggaa ggttttgaat tgggcgtatc
     1981 ccgccctgag gtagtcactc gggaagtgga cggcgaaatt cacgagccct acgaacaagt
     2041 agtgatcgac gtggaagagc aacaccaggg ttccgtgatg gaagagctgg gcctgcgccg
     2101 gggtgaatta accaatatgg tacccgacgg caaaggccgt gtgcgtttgg aatttattat
     2161 tccctcgcgc ggtttaatcg gttttcgcgg cagcttttta accatgacct ccggctctgg
     2221 catcatgacc agtatttttg atcattatgg cccggtaaaa ggcggcaata tggccaagcg
     2281 ccaaaacggt gtattggtga gtatggtgcg cggtaaaact ctggcttatg cgttgtttgc
     2341 cctgcaggag cgcggccggt tatttttagg ccacgctgaa gaagtctacg aagggcagat
     2401 cgtcggcctg cacagccgcg ataacgacct agtagttaac cccactaaag ccaagcagct
     2461 caccaacatt cgcgctgcgg gtactgacga agctctggta ttaacgccgc atattcgcca
     2521 tacattggag caagcgttgg aatttattga agatgatgaa ttggtggaag taacccccaa
     2581 aagcattcgc atccgcaaaa aattactgac ggaaaacgag cgcaagcgcg catccaaaag
     2641 cggcagttaa ttgatagtta ttacccaata aaaaaggcgc ctaggcgcct tttttattgg
     2701 ctgaataatt tacatgccgt gtacggcata aatgcccggc gcattgcgcc aaagccccga
     2761 ataatccatc cccatgccaa atacatagcg atcgggcacc cataaggcgc taaattcggg
     2821 gcgaaaattg gcgggtttgc gtgggtggtc cttagtggtc atcactgcgc agtgcacaga
     2881 actcgcccct tggtgcaaaa gttcttgata caccgcatgc agggttttgc cctcatcaaa
     2941 aatatcgtcg cacagcagaa tggttttgcc ttgaatgtcg gcatgctggg gcgaaatcca
     3001 gcgcagctcg gcaccggcca cattattgtg gtagcgggta gcgtgcacgt agctgagctc
     3061 caccggtctt tttaaatgag gcaataattg cccggtaaat accaagccgc catttaatag
     3121 gcacagcacc aaaaggggct cgcagccggg aaggcggcta ttaatatcct gcgccagggc
     3181 tgctatggcg ctgctgactt gctcaaagga atacaactgc tcggaaaatt cacctaatcc
     3241 gtctgttgac gtttggctca taaatacctc aggccaaggt taaatgtgga cgccgctaag
     3301 cgctgcgaaa ttgttttaat gcactgcaaa attcgggttt taactgcaga gcgatataaa
     3361 aagtgtatgc aagaattttt ggctgcgctg gaaaacttaa aaaatcttag cgcccacggg
     3421 cttcctcacc tatcccatta aatggtttgc cagtgggtgt agcaataaat tattgacgag
     3481 aaattgctag cgcccaggca gcccgctggc ttaagtgtta gataaatagc gagctatta
//
LOCUS       DAOQGS010000437         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15111_length_3534_cov_3.468525, whole genome shotgun sequence.
ACCESSION   DAOQGS010000437 DAOQGS010000000
VERSION     DAOQGS010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15111_length_3534_cov_3.468525"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKZ52_14220"
     CDS             <1..281
                     /locus_tag="PKZ52_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HNG60976.1"
                     /translation="SHTQPVQSPEEVPFLLLVPAFITSELKTAFQIGFIIFIPFLVID
                     IVVASVLMAMGMMMLSPLIISLPFKIMLFVLVDGWALIIGTLAASFGI"
     gene            295..564
                     /gene="fliQ"
                     /locus_tag="PKZ52_14225"
     CDS             295..564
                     /gene="fliQ"
                     /locus_tag="PKZ52_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNG60977.1"
                     /translation="MTPEVALDLFAQAFWLTITMVLVIVGPSLAVGLVVSIFQAATQI
                     NEQTLSFLPRLIVTLLTIMATGAWMINKLMELFSQLMLDIPHVIG"
     gene            567..1358
                     /gene="fliR"
                     /locus_tag="PKZ52_14230"
     CDS             567..1358
                     /gene="fliR"
                     /locus_tag="PKZ52_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNG60978.1"
                     /translation="MLPIVLTDQQIAQWLGQLFLPLTRFSGLLMLMPVIGTRLVPART
                     RMVLAFWLALISAPLLPALPPLDLLSLPTLLMLAEELLLGMMMGFIFMLVFQVFVLAG
                     QFMALKLGMGFSAMNDPTTGISVTSLSQFYLLLVTLCFIGMNGHILLIEGLLRSYQAF
                     PPGAIDLSREHFIKIALLSSWLFERALVMSLPVFAFLMVINIAFGVMSRSAPQLNIFA
                     VGFPFTVLCGLLMIGYGLNTFLADFGEIIDQGFAHLETIALTAKP"
     gene            1386..2519
                     /gene="flhB"
                     /locus_tag="PKZ52_14235"
     CDS             1386..2519
                     /gene="flhB"
                     /locus_tag="PKZ52_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNG60979.1"
                     /translation="MADDHSSEDKTEDATPRRQEKAREEGQIPRSRELTTALVLLVSL
                     VALWVFGGHIVRTMLVVFQRNFSISREAIFDPNIMAGYFATSLFDALWDLVAFAGLTL
                     LAALVAPIALGGWLFTPNSALPKLNRLDPMAGLKRMFSTKSLIELGKALAKVGLIIIF
                     SYAVIHGMLNAIAGLSHEPSAQAMEHMLSLCFWASIIIASSTLVIAGIDIPIQIWEYT
                     KKLKMTLQEVKDEMKDSEGRPEVKGRIRQLQREMAQRRMMSNVPKADIVITNPTHYAV
                     ALQYDPNKPGAPILLAKGTDQIALKIREIAKASGIESVESPALARSIFHTTQIDAEIP
                     TGLYVAVAQVLAYVFSLRNYRKGRGEKPQFPRNISVPRDLYFD"
     gene            2695..3441
                     /locus_tag="PKZ52_14240"
     CDS             2695..3441
                     /locus_tag="PKZ52_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60980.1"
                     /translation="MSILSVVVICGILMIASLLVVSFVNRQHTRKRVVTHKIWELRRR
                     INELEEVGAAIEPLVESPIIPKAINDEIIDLIHRVTALDPSIVYLESNLRTAQSLSAA
                     YMEQTAPSVLYRVQPSDAAIARAQHYLQEAGRIVRNQQIAGRIDITEMDQFIRELGWA
                     HLMVEVLSHIGQGHKALARSDVLSAYGYYRNAINKLMTSTVTDERKHRLIREVSEILQ
                     NKRKAISLDLMPESNFNPGANTPDLPLQGM"
BASE COUNT          835 a          903 c          853 g          943 t
ORIGIN      
        1 tctcccacac ccagccggtg caatccccag aagaggtacc gtttttatta ttagttccgg
       61 cgtttatcac ctcagagctt aaaacagctt ttcaaattgg ctttattatt tttatcccgt
      121 ttttagtgat cgacatagta gtggccagcg tgttgatggc catgggtatg atgatgctat
      181 cgccgctgat tatttccctg ccgtttaaaa ttatgttgtt tgtattggtg gacggctggg
      241 cgttaattat tggcaccctc gcggcaagtt tcggcattta acctaggaag ccccatgaca
      301 cctgaagtag ccctcgattt atttgcgcaa gcattttggc ttaccatcac catggtgcta
      361 gttattgtcg gccctagttt agcggtgggt ttagtggtga gcattttcca agcggccacc
      421 caaattaacg aacagacgtt aagttttctg ccgcgcttaa tcgtgacact tttgaccatc
      481 atggccacag gcgcttggat gattaataaa ctgatggagt tgttctccca gctgatgctg
      541 gatattcccc atgttatcgg ctgaatatgc tgccgattgt tttaaccgac caacaaattg
      601 cccaatggct ggggcaactt tttctgccct taacccgctt tagcggttta ttaatgttaa
      661 tgccggtgat tggtacccgg ctagtgcctg cgcgcacccg tatggtattg gcattttggc
      721 tggcgctaat cagcgcaccg ctgctgcccg ccctgccgcc attagacctg ctctcgctgc
      781 ccacgctatt aatgctcgcc gaagaattat tattgggcat gatgatgggg tttattttta
      841 tgctggtatt tcaggtattt gttttggccg gccaatttat ggcgctcaaa ttaggcatgg
      901 ggttttccgc tatgaacgac cccaccaccg gcatttcggt aacttcccta tcacaatttt
      961 atctgctgct ggtcacgcta tgttttatcg gcatgaacgg acatatttta ttaatcgaag
     1021 gtttactgcg cagttatcaa gcctttccac ccggcgcgat tgatttatcg cgggaacatt
     1081 ttattaagat tgcgctactg agcagttggt tatttgaacg ggccctggtg atgtcgctgc
     1141 cggtatttgc atttttaatg gtgataaata tcgcctttgg ggttatgagc cgttcagcgc
     1201 cgcaactgaa tatttttgca gtggggtttc cctttaccgt gctgtgcggc cttttgatga
     1261 ttggctatgg tttaaataca tttttagcgg attttggcga gattatcgac cagggctttg
     1321 cccacttaga aaccattgca ctgaccgcta aaccctaaac cttcaacaac ccgtgagcag
     1381 acgccgtggc agacgatcac agcagtgaag ataaaacgga agacgcaacg ccgagacggc
     1441 aggaaaaagc ccgtgaagaa ggccaaattc cccgctcgcg ggaactcacc accgctttag
     1501 tattattagt ttcattggta gccctgtggg ttttcggcgg ccatatagtg cgcaccatgc
     1561 tggtggtgtt tcagcgcaat ttttccatca gccgcgaagc catcttcgac ccgaatatta
     1621 tggcgggtta ttttgccact tcgttatttg atgccctgtg ggatttagtg gcctttgcag
     1681 gccttacgct cctggccgcc ttggtggctc ctatagcgct cggcggctgg ttatttactc
     1741 ccaatagcgc cctacccaaa ttaaaccgct tagaccctat ggcagggctt aagcgcatgt
     1801 tttccactaa atccttaatt gaactaggca aggctttggc caaagtaggt ttaattatta
     1861 ttttttccta cgctgttatt cacggcatgt taaatgccat cgcaggcctc agtcatgaac
     1921 ccagcgccca ggctatggaa catatgctct cgctgtgttt ttgggccagt attattatcg
     1981 cctcgtcaac cttggtcatt gcaggcattg atattcccat tcaaatttgg gaatacacca
     2041 aaaaattaaa aatgaccctg caggaagtta aggatgaaat gaaagattcc gaaggcaggc
     2101 cggaggttaa aggccgcatc cgccaattac agcgggaaat ggcccagcgc cgcatgatgt
     2161 ctaatgtgcc caaagccgat atcgtcatca ccaaccccac ccactatgcg gtggccctgc
     2221 aatacgaccc gaataaaccc ggtgcgccca ttttattggc caagggcaca gaccaaatcg
     2281 ccctgaaaat tcgcgagatc gccaaggcgt ccggtattga atcagtggaa tccccagccc
     2341 tggcccggtc aattttccac accacacaaa tcgacgcgga aattcccacc gggctttatg
     2401 tggcggtggc acaggtattg gcctatgtat ttagcctgcg caattaccgc aaaggccgcg
     2461 gtgaaaaacc gcaatttccg cgcaatatca gcgtgccgcg ggatttgtat ttcgattgac
     2521 gcaccctaac agcttgttga aatacgtagg cggcgacgca ctggcaaggt agtatttcaa
     2581 caagctgcta agcggcccca ttgagcgggc ctgccacatc cggcctacac ttacaccata
     2641 ccgtgcgccc cgattgggcg gcggccagtg ttataggcgg gggtttggta tttcatgagc
     2701 atcttaagcg tggtagtcat ctgcggtatt ttgatgatcg cctctttatt ggtggtcagt
     2761 tttgtcaatc gccaacacac ccgcaaacgc gtggtcaccc ataaaatatg ggaattgcgc
     2821 aggcgtatca acgaattgga ggaagttggc gccgctattg aacccttggt ggaaagcccg
     2881 attatcccca aagcgattaa cgatgaaatc atcgatttaa ttcaccgagt taccgccctc
     2941 gaccccagta ttgtgtattt ggagagcaat ttgcgcaccg cgcagtccct ctcggcggcc
     3001 tatatggaac agactgcgcc ctcggtgctg taccgcgtgc aaccgagcga tgccgccatc
     3061 gcccgcgcgc agcattattt acaggaggca ggcagaatag tgcgcaacca acaaattgcc
     3121 gggcgcatcg acattacgga aatggaccaa tttattcgtg aattgggctg ggcgcatttg
     3181 atggtggaag tactcagcca cataggccaa ggccacaagg ccctggcccg ctccgatgtc
     3241 ttaagcgcct atgggtatta tcgcaatgcc atcaataaat tgatgaccag caccgtcacc
     3301 gatgagcgca aacaccggct tattcgcgaa gtctcggaga ttttgcaaaa taaacgcaaa
     3361 gccatttccc tggatttaat gcccgaatcc aactttaacc ccggcgccaa cacaccggat
     3421 ttaccgctgc agggcatgta ggatgctcga ataaagccga tttaaacctg caaaaaacct
     3481 tattactagc tgtgcggaag caatgaatac acatcatttc agaccgtcaa aaag
//
LOCUS       DAOQGS010000438         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15130_length_3530_cov_5.301295, whole genome shotgun sequence.
ACCESSION   DAOQGS010000438 DAOQGS010000000
VERSION     DAOQGS010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15130_length_3530_cov_5.301295"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /gene="lipB"
                     /locus_tag="PKZ52_14245"
     CDS             complement(<1..477)
                     /gene="lipB"
                     /locus_tag="PKZ52_14245"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNG60981.1"
                     /translation="MSLVVKQLGLCAYTPVWEAMRAFTDRRDSSTPDEMWCVEHTPVF
                     TQGQAGKPEHLLSAGDIPLVASDRGGQITYHGPGQLVVYPLLDLRRRHLGVRQMVSLL
                     EQSCIDLLAHWQIQAHAKADAPGVYVGGGSLPERKIASLGLRVRKGCCYHGISINVA"
     gene            complement(478..741)
                     /locus_tag="PKZ52_14250"
     CDS             complement(478..741)
                     /locus_tag="PKZ52_14250"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNG60982.1"
                     /translation="MSQPPLLEFPCSYPIKVMGAAGEYFTEQVLAVFSQHAPGFDREQ
                     VRIQPSAKGNFVSIHVVIEAQGAEHIEVIYQALKTLPDLKLVL"
     gene            complement(743..1906)
                     /locus_tag="PKZ52_14255"
     CDS             complement(743..1906)
                     /locus_tag="PKZ52_14255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNG60983.1"
                     /translation="MPKLFLSPLFFSVLLFSVGAVADGPLIPAPPTIAAPAYLLMDAT
                     SGSVLLEQNADVQRPPASLTKMMTSYIAVGDVEKGKLKETDLVHVSEDAWRRGGAVTE
                     GSTMFLEVNSDVSVIDLLRGVIIQSGNDASIALAEHIAGSEPAFADIMNQQAKQLGMT
                     NTHFENATGLPGDNHKTTARDLAILARAVINDHPAHYPLYAERWFNYKGHNQPNRNRL
                     LFRDSTVDGLKTGHTQEAGYCLVASAKRNDMRLITVVLGSASEETRAVESQNLLAYGF
                     RYFEGANIFHKTQELAATRVWKGKANKISLGVGKDVSVTIQKGGKDRLTVQTTVANPI
                     IAPISPGQELGQVVVSLDGKEVFKGPLVAMTGIEQAGFFGRLWDSLMMLFAGV"
     gene            2320..3150
                     /locus_tag="PKZ52_14260"
     CDS             2320..3150
                     /locus_tag="PKZ52_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="HNG60984.1"
                     /translation="MDQYELMTKALTPLSNLATFEEEPPSPTTPPSGLVFYVVGDRFE
                     RFCDHNVVLNYAEALEQHKIIARLASEIVIGQGIGAQQLQALQTAFATADYRHSIVMT
                     QRLSEKVPAALVDKRDAKNVLVTRPTQNGPLYFRSQLVVDDHCVEFADCTDGEHLPGT
                     LLIEAAKQLFIASIRTHKPAPLNGTALDEMRFTLTGIQVQFEHFVFPIAAELHLTLQD
                     IEITNLRAKGTAIITIEQLGRRCCQIIFHAHAYSETVAESLEHRCGLKIKNRLLAANL
                     "
     gene            complement(3299..>3530)
                     /locus_tag="PKZ52_14265"
     CDS             complement(3299..>3530)
                     /locus_tag="PKZ52_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60985.1"
                     /translation="KVILPSGAGVLHNSTKSHLNRTVHQGFADNQNMTPRMRHAYILV
                     GINLKTAMEPLKNYLTRPGGVEFGKKMLIYCV"
BASE COUNT          900 a          942 c          877 g          811 t
ORIGIN      
        1 ggccacatta atactgatgc cgtggtagca acaacccttg cgcacccgca accccaagga
       61 ggcaattttg cgctcaggca ggctgccgcc gcccacatat acgccggggg catcggcttt
      121 agcgtgggcc tgaatctgcc aatgggcgag caggtcaatg caggattgct ccaacaggct
      181 caccatctgc cgcacaccca gatggcgacg gcgcaaatcc aacagcgggt acaccactaa
      241 ctggccaggc ccatggtagg taatttgccc gccccggtcg ctggccacca atggaatatc
      301 acctgcgctg agcagatgct caggcttgcc cgcctggccc tgggtaaaca ccggtgtgtg
      361 ttcaacacac cacatttcat cgggtgtgct tgaatcacgc cggtcggtaa aagcgcgcat
      421 ggcctcccac accggagtat aggcacacag gcccaactgc tttaccacta ggctcaatta
      481 caacaccagt tttaaatcgg gcagggtttt aagggcctgg tagatgacct cgatgtgctc
      541 ggcgccctga gcttcaatca ctacatggat ggagacaaaa ttgcctttgg cggagggctg
      601 aatgcgcacc tgctcgcgat caaaaccggg ggcatgttgg ctaaataccg ccaatacctg
      661 ctcggtaaaa tactcccccg cagcgcccat caccttaatc ggatagctgc agggaaactc
      721 caaaagcggc ggctggctca tgctaaaccc cggcgaataa catcatcaaa gaatcccaca
      781 aacggccaaa aaagcccgcc tgctcaatac cggtcatggc caccaatggg cctttgaata
      841 cttctttacc gtccaaactc accaccactt gccccagctc ttgccctggg cttatagggg
      901 caataattgg gttggcgaca gtggtttgca cggttaaacg atctttacca cctttttgaa
      961 tagtgacgct tacgtccttg cccaccccta aggaaatttt attagctttg cccttccata
     1021 cacgggtggc agccagctcc tgggttttgt ggaagatatt ggcgccttca aaatagcgaa
     1081 agccgtaggc caaaagattt tgtgattcca ccgcgcgggt ctcttccgag gcactgccta
     1141 aaaccacggt gatcaagcgc atatcattgc gcttggcaga agccaccaaa caataccccg
     1201 cctcttgggt gtggccggtt tttaagccat ccacggtgct atcgcgaaat aataggcggt
     1261 tgcgattggg ctgattgtgg cccttgtagt tgaaccagcg ctcggcgtac aaagggtaat
     1321 gggccgggtg atcattgatc actgcccgcg ccagaatggc caaatcccgc gcggtggttt
     1381 tatggttgtc gccgggcaag ccagtggcat tctcaaaatg ggtattggtc atgcccagct
     1441 gtttggcctg ctggttcatg atgtcggcaa aggccggttc tgaacctgca atatgctcag
     1501 ccagtgcaat ggacgcatcg ttacccgact gaataataac cccgcgcaac aggtcaatta
     1561 cggatacgtc ggaattgacc tccaaaaaca tggtagagcc ttccgtcacc gccccacccc
     1621 gccgccaggc atcttcagag acgtgtacca ggtcggtctc tttgagctta cctttttcca
     1681 catcccccac cgcgatgtag ctggtcatca ttttggtaag gctcgccggt ggccgctgca
     1741 catcggcgtt ttgttccaac aatacgctgc cactggtggc gtccataagc aaataagccg
     1801 gggcggcaat ggtaggagga gcgggaatca atgggccatc ggccacagcc ccgacagaga
     1861 agagcaaaac actaaaaaac aagggggaaa ggaacaattt aggcatgaaa ttctgcttgg
     1921 aaattaaaaa agctgcgcat tgtaccaggt gtgatgcagg caacaagcgc ggaagaagac
     1981 cgcttaggcg gaatttaagc ccagctttta aaagccgagg aattcatacc ggctcacata
     2041 gggtgaatag ttcacgcttt aggggctact ttatttacat tccacacggg gatttagcat
     2101 tactgtgcag caaagacggg attaacctaa aagccgcagt tgaactctac tgcggctgcc
     2161 taagacttga atctgcacgt tttttatcaa aatttggctt accgtagagt tgactactgc
     2221 tttcaccaca ttttgacaaa aaacacacat attcggcgcc ttaggcaccc tcgctacgag
     2281 ttcaactgcg gtttttaggt ttaaacgagg agattgacca tggaccaata tgaattgatg
     2341 actaaagcgc taaccccctt gagcaacctt gccaccttcg aggaagaacc accctcccct
     2401 acaacgcccc ccagcggcct ggtgttttat gttgttggcg accggtttga gcgcttttgc
     2461 gaccacaatg tagtgctaaa ttacgcggaa gcactggagc aacataaaat tattgcccgg
     2521 ctggcgagcg agattgtgat cggccagggc ataggcgcgc agcaattgca ggcgctgcaa
     2581 accgcttttg caaccgcaga ttatcgacac tctattgtga tgacacagcg gctcagcgaa
     2641 aaagtccctg cggcactggt ggataaacga gatgctaaaa atgtattagt cacacgccca
     2701 acacaaaatg gccccctgta ttttcgcagt caattagtcg tggatgacca ctgcgttgaa
     2761 ttcgccgact gtacggatgg tgagcatcta cccggcactt tattaattga ggcggctaaa
     2821 caacttttta tcgccagtat ccgcacccac aaacccgccc ctctaaatgg cactgcgctg
     2881 gatgaaatgc gctttactct tactggtata caagtgcagt ttgaacattt tgtctttcct
     2941 attgcggccg aactgcattt aaccttgcag gatattgaga tcaccaacct gcgcgcgaaa
     3001 ggcacggcca ttattaccat tgagcagctg gggcgcaggt gttgccaaat aatttttcac
     3061 gcccacgcct acagcgaaac agttgccgag agtctcgaac atcgctgcgg gctgaaaata
     3121 aaaaatcgct tactcgccgc taatttataa cagccgcgac ataatggttg atccctgctt
     3181 tgcgactgcc cggcctgcag aataaagaca ggtattttaa atcgttaaaa atagggaacc
     3241 tctgaaaaat gtaggcggcg aagctctggc taggcgaatt tggcaaaaaa agcggagttt
     3301 acacgcagta aatgagcatt tttttgccaa attcaacgcc gccagggcga gtaaggtaat
     3361 ttttcagagg ttccatagca gtttttaggt ttatgcctac aaggatgtag gcgtggcgca
     3421 tccgaggtgt catgttttga ttatctgcaa aaccctggtg aacagttcta tttaaatggc
     3481 tcttcgtaga attgtgtaaa acccccgcgc cagatggcaa aatgaccttc
//
LOCUS       DAOQGS010000439         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15207_length_3520_cov_2.879654, whole genome shotgun sequence.
ACCESSION   DAOQGS010000439 DAOQGS010000000
VERSION     DAOQGS010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15207_length_3520_cov_2.879654"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKZ52_14270"
     CDS             <1..246
                     /locus_tag="PKZ52_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNG60986.1"
                     /translation="QRAQRLGAALDAVVTCDTAIAHLAGALGKPTLLLLSSEGEWRWL
                     QNRADTPWYPSLRLLRQPRPGDWAGLAAPTLEWVRGR"
     gene            441..1763
                     /locus_tag="PKZ52_14275"
     CDS             441..1763
                     /locus_tag="PKZ52_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG60987.1"
                     /translation="MLLEFRVGNYRSIRDEQVLSLVASTTDEELEDTHLVPTGLKAFP
                     SAVRSAVVYGPNASGKSTLLFALNYMRAVVAESAAVIQPGQTYNVQPFKLDQAFAKAP
                     TTFEMTFLIDGVRHQYAFEMTSQRFVSESLVVYRTSKPTKWYSRRLMDDGETYEYEFS
                     AYLTGSRKLWQDSTRPNALFLSTAAQLNSELLSPVFRAIVDDIAYLPAGSAFDPEFTT
                     TLLTTEEGRAAIRDFLAAADISIADVQAVARKGLRQQIFLHAGGMAPSSPEERELLFP
                     IFQHTTTNGSAKFELHEESDGTQRLYGMAAPVIDVLKRGRTLVVDELDSSLHPLLVRR
                     LISMFHDPSINKAGAQLVFSTHDTSLLDHTLFRRDQIWFTEKDREQATRVYPLTDFSP
                     RKREAWENGYLTGRYGAVPFFSALPDILRHALPEPSTPDSEPASPLKA"
     gene            1786..2463
                     /locus_tag="PKZ52_14280"
     CDS             1786..2463
                     /locus_tag="PKZ52_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNG60988.1"
                     /translation="MGRENQPKHRQQARDLKRRAAQRAPYKRMLIVCEGEKTEPNYFG
                     EICQEYRLATANVQVWPSALGTQPLQVVNYAELLFRDGDRSKGIRPKAFDHVYAVFDR
                     DDHTTYHQALAKADSLRDKLRNDSGEVVAFQAIASAPCFELWLLLHFEDIHAPIHRDE
                     VYARLKRYLPDYDKGRSGYWAATQDRFEQAVARAENRAAATTAHDGNEPYTSVHKLIL
                     SLAHLKD"
     gene            complement(2623..>3520)
                     /locus_tag="PKZ52_14285"
     CDS             complement(2623..>3520)
                     /locus_tag="PKZ52_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60989.1"
                     /translation="SCLTGAEIAALAPDATYWNFIVDDLRLAEGIARCARDFPDLPRV
                     YGLDDRIGDLPPQHPYATRFSGAQLDRRLREALALCQRLVVSTPPLAELYGPLVESVR
                     VVPNRLERALWQGVAPPTREAAPPRRLRVGWAGAQQHAADLALLRPVIAATADTFDWI
                     LFGMAPAGCEAWVREIHPAVPFPDYPARLAALDLDIALAPLEINLFNEAKSNLRLLDY
                     GWLGWPVICTDILPYRENAPPVIRVANTPEAWTTALHALATDPDTRHRLGQELAAWVR
                     RHYVLEDHLEEWREALVPGWGG"
BASE COUNT          739 a         1035 c         1083 g          663 t
ORIGIN      
        1 cagcgggcac agcgcctcgg tgccgcgctg gatgccgtgg tcacctgcga caccgccatc
       61 gcccacctgg ccggcgccct gggcaaaccg accctgctcc tcttgtccag cgaaggcgaa
      121 tggcgctggc tacaaaatcg cgccgacacc ccgtggtacc cgagcctgcg cctgctgcgc
      181 cagccaaggc cgggtgactg ggcgggacta gcggcgccga cgttggagtg ggtgaggggg
      241 aggtaggggg gcggctcgtc ggcctgagcg gtcattcgac atcatttggt tatacggccg
      301 ccaagtagcg ggttgcggcc cttaaaggat ggtgatcggg gatgcctaga gaacgtaaaa
      361 accggtacgg tacaaataat tattaaaatt ttgcacctaa tgtacgataa ttaatgaaat
      421 attagtactg aggagccgtt atgctattgg agtttcgggt cgggaattac cgctccatcc
      481 gcgatgagca ggtgctgagt ctcgtcgcct cgacaactga tgaagagttg gaagacaccc
      541 atctggtgcc cacgggactg aaggccttcc cgagcgctgt acgcagtgcc gtggtgtacg
      601 gtcccaatgc cagcggcaag tcaactttgt tgtttgcgct gaattacatg cgggctgtag
      661 tggcagagtc ggcggctgtc attcagccgg ggcagaccta caacgtccag ccttttaagc
      721 tggatcaagc cttcgccaag gcgcccacga cgttcgagat gactttcctc attgatgggg
      781 tgcgacacca gtatgccttc gagatgacgt cgcagaggtt cgtgagcgag tccctcgtcg
      841 tatatcgcac ctctaagccg actaagtggt acagccgtcg attgatggac gatggcgaaa
      901 cctacgaata cgagttcagc gcttacctca ccggttcgcg caagctctgg caagacagca
      961 cgcgtcctaa cgcgctcttc ttgtcaacag ctgctcagct caacagtgag ttgcttagtc
     1021 cggtattccg agcgattgtt gacgacattg cgtacctgcc ggcaggctcc gcttttgatc
     1081 ctgaatttac aaccaccttg ctgaccaccg aggaggggcg cgctgctatt cgcgattttc
     1141 tcgcggccgc agatatcagc atcgcagatg tacaagctgt tgcgcgcaag ggactccgcc
     1201 aacaaatttt cctccacgca ggtggcatgg caccaagcag ccctgaagag cgcgaactcc
     1261 tctttcctat ttttcagcac accaccacca atgggagtgc caagttcgag ttgcacgaag
     1321 aatctgatgg cacgcagcgc ctctacggaa tggctgcccc ggttatcgac gtgttaaagc
     1381 gtggccgcac cttggtggtc gatgaactcg atagcagcct tcaccccctt ctggtgaggc
     1441 gtctcatctc gatgttccat gatccttcta ttaacaaggc cggtgcgcaa ctcgttttca
     1501 gtactcacga cacttcactg ttggaccaca ccttattccg acgcgaccag atatggttca
     1561 ccgagaagga cagagaacaa gccacaaggg tctatccgct caccgatttc agtcctcgca
     1621 agcgcgaagc ttgggaaaac ggctatctaa cgggcagata cggagctgtg cccttcttta
     1681 gcgcattacc ggacatcctg aggcacgcgc tgcccgagcc aagcactcct gattcagagc
     1741 cagccagccc ccttaaggct tgagccagag ctacaggaga actgcatggg acgagagaat
     1801 caaccaaagc accgccaaca ggcgcgtgac ctcaagcgtc gcgctgcgca gcgggcgccg
     1861 tataaacgca tgctcatagt gtgcgaggga gaaaaaacag aaccaaacta cttcggagag
     1921 atttgccaag aatatcgact ggcaacggct aacgtgcaag tgtggccgag cgcgctcggg
     1981 acacaaccac tacaagtggt taactacgct gaattgttgt ttagggatgg cgaccgcagc
     2041 aaaggcatcc ggcccaaagc ttttgatcac gtttacgcag ttttcgaccg cgacgatcac
     2101 accacctatc accaggcact cgcaaaagcc gattccctac gagataagct cagaaacgat
     2161 tccggtgagg ttgtcgcgtt ccaagccatc gcctccgcgc cctgtttcga actgtggtta
     2221 ttgctgcatt tcgaagatat ccacgccccg attcaccgcg acgaagttta tgcacggctg
     2281 aagcgctatc tgccggatta cgacaaaggc cgtagcggtt actgggcggc cacccaagac
     2341 cggttcgaac aggctgttgc ccgagctgaa aaccgtgctg ctgccaccac agcccacgac
     2401 ggcaacgaac cctacaccag cgtgcacaag ctaatattgt cgctcgccca tctgaaagac
     2461 tgatcctgtt tggagtaccc cgaatcactt cccgcatgca tcatcagaaa aaatagcggg
     2521 caattcctct tctagactgc caaggatttc agcgaccgca cttagttcag gaccagccac
     2581 tcaactacga cgtcgccaag gacagcaaag gccgaaaagc actcaccctc cccaacccgg
     2641 aaccagcgcc tcccgccatt cctccaggtg gtcctccagc acgtaatgcc gccgcaccca
     2701 ggcggcaagt tcctgaccca ggcggtggcg ggtatccggg tcggtggcca gggcgtggag
     2761 ggccgtggtc caggcttccg gggtgttggc cacccggatc accggcgggg cgttttcccg
     2821 gtagggcagg atgtcggtgc agatgaccgg ccagccgagc cagccgtaat cgagcaggcg
     2881 gaggttgctc ttggcttcgt tgaagaggtt gatctccagg ggggcgaggg cgatgtccag
     2941 gtccagggcg gcgaggcggg cggggtagtc ggggaagggg acggcggggt ggatttcccg
     3001 cacccaggct tcgcagccgg cgggggccat gccgaagagg atccagtcga aggtgtcggc
     3061 ggtggcggcg atgacggggc gcagcagggc caggtcggcg gcgtgttgct gggcgccggc
     3121 ccagccgacc cgcaggcgcc ggggcggtgc cgcttcccgg gttgggggcg ccacaccttg
     3181 ccacagggcg cgttccagac gattggggac gacgcgcacg gattccacca gggggccgta
     3241 gagttccgcc aggggcgggg tcgagactac cagccgctgg cagagggcga gagcttcgcg
     3301 caggcgccga tcgagctggg cgccgctgaa gcgggtggcg taggggtgtt gcgggggcag
     3361 gtcgccgatg cggtcgtcca ggccatagac ccggggcaga tcggggaaat cccgggcgca
     3421 gcgggcgatg ccttcggcga ggcgtaggtc gtcgacgatg aaattccagt aggtggcgtc
     3481 cggggccagg gcggcgattt ccgccccggt gaggcagctc
//
LOCUS       DAOQGS010000440         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15210_length_3519_cov_4.007794, whole genome shotgun sequence.
ACCESSION   DAOQGS010000440 DAOQGS010000000
VERSION     DAOQGS010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15210_length_3519_cov_4.007794"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..443)
                     /locus_tag="PKZ52_14290"
     CDS             complement(144..443)
                     /locus_tag="PKZ52_14290"
                     /inference="COORDINATES: protein motif:HMM:NF021222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HNG60990.1"
                     /translation="MKKIILCCVALSCSQFVLAGDGTTELERKNLIVIAKQLNQLHEL
                     ANEAEAASDKDARVKFNYQMLHKDITAMSQAIQNHINKPSRTPRAVPELNQSYSN"
     gene            complement(440..934)
                     /locus_tag="PKZ52_14295"
     CDS             complement(440..934)
                     /locus_tag="PKZ52_14295"
                     /inference="COORDINATES: protein motif:HMM:NF022523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HNG60991.1"
                     /translation="MRSQWIFLISCIAISAQIAAADPIVIADHGGTPTGVPTYETVRQ
                     EYLNSAPAVPQEQAFNPYPILSEIKPGKLARPLRAKAPIDTPIFFIGPDDFSVKWAQQ
                     NKQYLLNISAAGYATNLISEQQFIDLQNQIKPLPLAPMPLDQVAELFGIPVYPVLLFK
                     EIRQ"
     gene            complement(934..1551)
                     /locus_tag="PKZ52_14300"
     CDS             complement(934..1551)
                     /locus_tag="PKZ52_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNG60992.1"
                     /translation="MKVLLCLASVICALESQNVMGNNAAAVPISYAQMPNAYIQISQQ
                     YGIPSEVFFSVMMQESSSKINIKSRNIWVPWPWTLNVELKPYYFSNKQEAKTALRQFL
                     ATDNKRIAVGIGQIYLPSHGHLFSDPTVLLEPEINLHYAAKLLADNFQWTIDRGNPNW
                     WTAVGRYHTPSRPDLAIPYSENVYARCKRISPHCDRFGTVSKGIH"
     gene            complement(1551..2333)
                     /locus_tag="PKZ52_14305"
     CDS             complement(1551..2333)
                     /locus_tag="PKZ52_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60993.1"
                     /translation="MRLKHLKISWVITAGLLTANACFSDIPLYNEDGSLRKIPAASYA
                     STQANSKRFLNPVIGQPAWDYWGLTESEWRRYEELKKSSPWAQWENTATPLAILAHYA
                     DPDTRQRYARIEAELDQWRFNQTMEYQRVYNREREIVNAAYQMAWEKRAPLIGNIGTA
                     DRTLFFVPKGVCEARCIALINPLLRTGSHLDIYVVGATEESEVFAWAKSAAIPPERVQ
                     TKHITLNFETGILKDLNPLPVGLIDLPIAYWHREGRYEKVIF"
     gene            complement(2348..3265)
                     /locus_tag="PKZ52_14310"
     CDS             complement(2348..3265)
                     /locus_tag="PKZ52_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60994.1"
                     /translation="MNTDQFDNSMPPEYLEQDTRIRETHSRNSIDLDDDENDLESDNK
                     PARDNRRSRAHREPKKTGKPWLSMMALLILIGFLVYRFLYQPPDTVKEGDTFGSSIVS
                     EVKQIAGDLESLPPKSAIEKLPIEAPKVTAVDVPGGNTPKDSTAHLESMIDAIRKNQE
                     LQMEILKDLKDASTRLEEKQRLHEDYLKSLTDKISSVPKKQSPSKQLAEGVNTLQKRT
                     KPTPSKITSTTQSLIAPVKPAVVFPDIRVTSIKNYNGKAGANLYVRGEPSPFILVGQN
                     WNGITLVGADTSTRSVSIEYFNEIRSYSL"
     gene            complement(3274..>3519)
                     /locus_tag="PKZ52_14315"
     CDS             complement(3274..>3519)
                     /locus_tag="PKZ52_14315"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HNG60995.1"
                     /translation="IGLVALIDGLTERDIRKMCGGNESAMRYHHAKRFIFPSVALAFG
                     FYLTLPITIHPAFVFLPIMLVTGLLIYTTASTFKKFL"
BASE COUNT          848 a          761 c          856 g         1054 t
ORIGIN      
        1 cccagaaaac aaaccagcgt tgccaataga aaaaaataca agcgcgaggg ttgtccaaat
       61 agctggataa attgatcgtt ggttgagcca ttcatgggta tgtgtgctgc atatctttta
      121 ataaagtttt ttggggttaa aacttaattg ctgtagctct ggttaagttc cggtacggcc
      181 ctgggtgtcc tcgatggctt gttaatatgg ttttggatcg cttggctcat ggcggtaatg
      241 tctttatgca acatctgata attaaacttc acacgcgcat ctttatcaga tgccgcctct
      301 gcctcgttgg ctagctcatg gagctggtta agttgcttgg cgatgacaat tagatttttg
      361 cgctccaatt ctgtcgtgcc atctccggcc agaacgaatt gactgcagct aagtgctacg
      421 caacacagaa tgattttttt cattgtcgaa tctctttaaa tagcaggacg ggatatacag
      481 gaataccaaa taattccgcg acttggtcaa gcggcatggg ggccagcggg agtggtttta
      541 tctgattttg aaggtcaata aattgctgtt cactgataag atttgttgca taacccgccg
      601 cactgatatt gagcaggtat tgcttattct gctgtgccca cttcacggaa aaatcatcgg
      661 ggccaatgaa aaatatgggt gtatcgatag gcgctttggc cctcagtggg cgcgccaatt
      721 tgccaggttt tatttctgac aggatcgggt aaggattaaa ggcttgctct tgtggaacag
      781 cgggcgctga atttagatat tcctgccgaa cggtttcgta agtgggcaca cctgtcggtg
      841 tgcccccgtg gtcggcaata acgatcggat cagccgccgc aatctgagca ctaattgcga
      901 tacagctgat taaaaaaatc cattggctac gcattagtga atacccttgc taacagttcc
      961 aaaacgatca caatgcggtg agatccgttt gcatcgtgca taaacattct cactgtacgg
     1021 aatcgctaaa tctgggcgcg atggtgtgtg gtagcggccc acggctgtcc accaattagg
     1081 attccctcga tcaatcgtcc attggaaatt gtcggcgagt aattttgcgg cgtaatgcaa
     1141 attaatttct ggctcaagta gtacggtggg atcactaaat aaatggccgt gcgatggaag
     1201 gtagatttgc ccaatcccaa ccgcaatacg tttattgtcc gtggccaaga attgccgtaa
     1261 ggccgttttg gcttcttgtt tattcgagaa ataatagggc tttaactcga cattcagtgt
     1321 ccatggccaa ggcacccaaa tattgcggct cttaatgtta attttactgc tgctctcctg
     1381 catcatcacg ctaaaaaata cttctgaagg gatgccgtat tgctgtgata tttggatgta
     1441 ggcattaggc atctgtgcgt atgaaatagg tacggcggca gcgttatttc ccataacatt
     1501 ttgagactct aatgcgcaaa taaccgatgc caaacacaat aaaactttca ttagaaaatc
     1561 actttttcat atcggccttc ccggtgccag taggcaatcg gtagatcgat aaggcctaca
     1621 ggtaagggat tcaggtcttt gagtattccg gtttcaaagt tcagcgttat atgttttgtt
     1681 tgcacgcgct caggtggtat agcggcagac ttagcccagg caaacacctc agattcttca
     1741 gtcgccccta caacataaat gtccaggtgt gatcctgttc ttaacagagg attaatcagg
     1801 gctatacagc gtgcttcgca aacgcctttg ggtacaaaaa acagcgttcg atcagcagtg
     1861 ccgatattgc cgatcagcgg tgcccgtttt tcccacgcca tttgataggc ggcgttcaca
     1921 atttcgcgtt ctcggttgta tacgcgctga tattccatcg tttgattaaa tcgccattgg
     1981 tcgagttcag cctcaattcg tgcgtagcgc tggcgtgtat cgggatcagc gtaatgcgcg
     2041 agaatggcga gcggtgtggc cgtgttttcc cattgcgccc agggactgga tttttttagc
     2101 tcttcgtagc gacgccactc cgattcggtt aacccccaat agtcccaggc aggttggcct
     2161 atgaccggat tgaggaatct ttttgaattt gcttgtgtag aggcataaga tgcagcgggt
     2221 atttttcgca gactcccgtc ttcgttgtat aacggaatat cactaaagca ggcattggcc
     2281 gttagtaagc ctgctgtaat gacccaactt atttttaagt gtttaagcct cataatcgat
     2341 ccttaattta aagactataa cttctgattt cattgaaata ttcgatgctg acacttcggg
     2401 tcgaggtgtc tgcacccacc aacgtaatac cgttccaatt ttgcccgacc aaaataaacg
     2461 gcgatggctc tccgcgcaca tacagattgg cgccagcctt gccgttatag ttttttatac
     2521 tggtaactcg aatgtctgga aaaaccactg caggttttac aggggcgatg agcgactgtg
     2581 ttgtactggt tattttgctc ggtgtgggtt tggtgcgttt ctgtagtgtg tttacgcctt
     2641 ctgctagctg ttttgatggg ctttgttttt tgggaacgct tgagattttg tctgttagtg
     2701 attttaaata gtcttcatga aggcgttgct tttcctctag tcgagttgag gcgtctttca
     2761 ggtcttttag aatctccatt tgaagttctt gatttttgcg tatcgcatca atcatcgatt
     2821 caagatgtgc ggtgctgtct ttgggggtat tgccgccggg cacatccact gcagtaactt
     2881 taggggcttc gatcggtaat ttttcaattg cgcttttggg cggtaaggac tccaaatcac
     2941 cagcaatttg tttgacttca gatactatgc tggatccaaa cgtgtcgcct tctttcacgg
     3001 tatcaggtgg ctgatataag aatctgtaca ccaaaaagcc aattaaaata agcaaggcca
     3061 tcatcgacag ccacggcttt cccgtttttt taggttcgcg atgcgccctt gatcgacggt
     3121 tgtccctggc tggcttgttg tcagattcca agtcgttttc gtcgtcatct aagtcaatgg
     3181 aatttcgact atgtgtctcg cggattctag tatcctgttc aaggtattca ggtggcattg
     3241 agttatcgaa ttgatcagta ttcatttata aactcacaga aatttcttaa aggtggatgc
     3301 ggttgtatag atcaacaggc cggttaccag catgatcggc aagaaaacaa atgctggatg
     3361 gatggtgata ggaagcgtta ggtaaaatcc gaaggcgagt gctactgacg gaaaaataaa
     3421 cctttttgca tggtggtaac gcatcgcgct ctcattgccg ccgcacattt ttcggatgtc
     3481 gcgttctgtt aggccatcga ttaatgcgac tagcccaat
//
LOCUS       DAOQGS010000441         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15294_length_3507_cov_4.028100, whole genome shotgun sequence.
ACCESSION   DAOQGS010000441 DAOQGS010000000
VERSION     DAOQGS010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15294_length_3507_cov_4.028100"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(677..1990)
                     /locus_tag="PKZ52_14320"
     CDS             complement(677..1990)
                     /locus_tag="PKZ52_14320"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNG60996.1"
                     /translation="MSGRLLSALINKAEIGILREVSGLWSFQYTQEWLNDPQSFALSP
                     HIPLSGESLIDGASTRPVQWYFDNLLPEEGQRVLLAGDAKLEVADAFGLLAWYGAESA
                     GSVTLLPSDSAPQTAQPLRPLPDEVLETRICQLPKASLTHGAIKRMSLAGAQHKLAVV
                     LHDGKLYEPAGATPSTHILKPDHPDDDYPHSVINEWFVMLLAKRLGLEVPNVHRRYVP
                     SPVYLIDRFDRFFDGQEWQRRHVIDACQLLGLDRSFKYSQGSMENLAALANACRSPAV
                     ARSRLFSWLVFNALIGNSDAHLKNLSFLVTHEGVQLAPFYDLLSVATYDTAAFDKMDW
                     PLETQLAWPILGVRHFSDVNRALLIKAGASLNLAKGTAERLLEHVRSRAVYEAEMLYA
                     KIEAENAQLAQLNPELSATMVGEARCIRAIIYTVLKEMAKQIARA"
     gene            complement(1987..2253)
                     /locus_tag="PKZ52_14325"
     CDS             complement(1987..2253)
                     /locus_tag="PKZ52_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG60997.1"
                     /translation="MKIPIDSAAAVGRVVRASRKAQKIRQDDAAGSIGVSENFLGKIE
                     RGSEAVQWGKLFQVLEGLGIRVSLDVPESVAALLNDPVNIKVKQ"
     gene            complement(2533..3192)
                     /locus_tag="PKZ52_14330"
     CDS             complement(2533..3192)
                     /locus_tag="PKZ52_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60998.1"
                     /translation="MQLKNMTLAQLKGLEQHIPSIRGLVGLPLVESYEEFLEVLYSDL
                     DCCNKLLVEHRQALQNDNEDRLSIQIVTSLKFKGYIAEHDTQHGGHVDIFVKHNQFTW
                     IGEAKIHGTYDYLMEGFLQLTTRYSAGVYNETSGGLFIYIRNKDAKAVVDAWKEHLSC
                     AFADKIEFIDCTYNPDCFYSSIRHSVSGKQYSIRHMPVALHFEPQDKSARTAKSKLPK
                     D"
     gene            complement(3182..>3507)
                     /locus_tag="PKZ52_14335"
     CDS             complement(3182..>3507)
                     /locus_tag="PKZ52_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG60999.1"
                     /translation="RSFQEVCHLTYYSFLTLLGSGFSGEVLIDTLRYLTGNRANVLKT
                     HFELIDEMDNAYVLDPPLVLQLQKNGTLYHPVTGELVSDPDNKVHLFFSPSDDFKELQ
                     ANNAA"
BASE COUNT          978 a          899 c          749 g          881 t
ORIGIN      
        1 atttcaccat ggtcgaacgg ctggcggctg tattgaaagt ccccgaggcc tatttttatg
       61 caaaggaaga tgaactcgct caactgctcc gagcctggcc actgctcaaa aaagcacagc
      121 ggcaaaccat caacgaagca attagagagc tcttaaccta gaaccaaaac cccttctaag
      181 cgcatccact catggcaatt gttgagtgga ctcccctacc aatgacacaa tcaaaccgct
      241 tgcaaagtcc ggctgtaagt tattgatttt attgggggca aatttatgaa actagcggac
      301 ttcctatttt ggtgcacggg agaattcgga gaataagacc gaggagagag aggaaaacgc
      361 cgaaatgttt aaaaaatagg cgccgattgg aatttgtaga aatcctcaaa accccctaaa
      421 tatccgcaga aatacgcact aacaggcaat aaaaaacccc aactgtctcc agttggggtt
      481 tgttttttgg tggtgggacg cccacccgca tcaaacgcca tcagcacaag tgattgggat
      541 taatgctaac acttaagggc ttacagggcc aagcacttac gccctcagaa agtaagcgtc
      601 caccaacata tttccacccc accgttagac ggtaacagtt caactccccc tatggaatct
      661 gaacccaaca cactggctaa gctcgggcga tctgctttgc catctcttta aggacggtat
      721 atatgatagc cctgatacac cgggcctcac ctaccattgt cgcggaaagc tcagggttca
      781 gctgggcaag ctgagcattt tcagcttcaa tcttggcgta aagcatctcc gcctcatata
      841 ccgcacgact tcgtacatgc tccaacagac gctcggccgt gcctttagcc aagtttagcg
      901 atgcgcctgc cttgatcagt aaggcacggt taacgtccga gaagtggcgt accccgagaa
      961 taggccaagc caattgggtc tctagaggcc aatccatttt atcgaaagca gccgtgtcat
     1021 atgtggccac gctgagtagg tcatagaacg gtgccagttg caccccctca tgagtgacca
     1081 aaaagctcag gtttttaagg tgagcatcgc tattgccgat gagtgcatta aacaccaacc
     1141 agctgaataa cctagagcgc gctaccgccg gactacgaca ggcattcgcc agggcggcga
     1201 gattctccat gctgccctga ctgtatttga aactccgatc caatcccagt aattggcagg
     1261 catcaattac atgtcgtcgt tgccactcct gcccatcaaa gaagcggtcg aaacggtcaa
     1321 tcaggtacac cggcgaaggc acatagcgac ggtgtacatt aggaacctca agcccgagcc
     1381 gtttggccag cagcatgacg aaccattcgt tgatgactga gtgaggataa tcatcgtctg
     1441 ggtgatcagg cttcaaaatg tgggtggacg gtgttgcacc agcaggttca tacaacttgc
     1501 catcgtgtag gacaaccgct agcttgtgct gagcacccgc cagcgacatc cgtttgatcg
     1561 caccatgagt caatgatgcc ttgggcaact ggcaaatgcg ggtttccaaa acttcatccg
     1621 gtaatggccg cagaggctga gccgtttggg gcgcagaatc cgacggtaac aaagtcaccg
     1681 accccgccga ttccgcgcca taccaagcta gcaatccgaa ggcgtctgcc acttcaagct
     1741 tggcatctcc agcaagcaaa acccgttgcc cttcttctgg caataaatta tcaaaatacc
     1801 attgcacggg gcgcgtgctt gctccatcta ttaaggattc accgctcagc gggatgtgcg
     1861 gactcaacgc aaagctctgc gggtcgttta accactcttg ggtatattga aagctccaga
     1921 ggccgctcac ctcccgcaga atgccgattt ctgccttatt gattaaagca ctcagcaacc
     1981 gaccgctcat tgcttgacct taatattcac cgggtcgttc aatagggccg ccacactttc
     2041 tggcacatct aggctaactc gaatgcccaa tccctcaagc acttggaata gcttgcccca
     2101 ttggacggcc tcggaaccac gttcaatctt gcctaggaaa ttctcgctaa cgccgatact
     2161 acccgctgca tcgtcttggc gaattttttg ggccttacgg cttgctcgca ccaccctgcc
     2221 aacggcggct gcgctatcaa tcggtatctt catacaaaat cctcacgaac gaatggattc
     2281 tgcaccaact ctagtcttta ggcaagaaat cctttcattc gtgcggattt ttaatagaag
     2341 tgatctttta cagtaaaatt cctcacagag gtgaggattc cacaaccata cagaaagatt
     2401 taataattac tttaaacttg agctacgatt gcagccagtc atgaaggcgc ttcctttcgg
     2461 taggaactcc aagtgatgaa atgaaggtta tttacaacga atatctctca tctttttctg
     2521 cctctagagc atttaatctt taggaagttt tgattttgcc gttcgtgcac ttttgtcttg
     2581 aggttcaaag tgaagtgcga ctggcatatg gcgtattgaa tattgtttac cactgacgga
     2641 atgccggata gaggaataaa aacaatcggg attataagtg caatcaatga attctatttt
     2701 atccgcaaaa gcacaactca gatgttcctt ccacgcatcg accactgctt tcgcgtcttt
     2761 attgcgaata tagataaaca aaccgccgct agtttcattg taaacaccag cagaataccg
     2821 ggttgtcaat tgcaaaaacc cctccataag ataatcgtat gtgccatgta ttttggcctc
     2881 gccaatccaa gtaaattgat tatgctttac aaatatgtct acatgaccac catgctgagt
     2941 atcatgctct gcgatatagc ctttgaattt taagctggtc actatttgaa tagaaagacg
     3001 atcttcattg tcattttgaa gagcttgcct atgctcaaca agcaacttat tacaacaatc
     3061 aagatctgaa tataaaacct ccaaaaactc ttcataactc tcaaccaaag gtaagcctac
     3121 aagcccccta attgaaggga tatgctgctc aagacctttt aattgcgcaa gcgtcatatt
     3181 tttaagctgc attattcgcc tgcaattctt taaagtcgtc gctaggagag aaaaataaat
     3241 gcactttatt atcaggatcg cttaccaact ctccggttac cggatgatac aaagtgccgt
     3301 ttttctgcaa ttgcaaaacc aatggcgggt ccaacacata tgcattgtcc atctcatcaa
     3361 taagttcgaa atgtgttttt aatacatttg ctctattgcc cgtcaaataa cggagagtat
     3421 ctattagcac ctcaccagaa aaaccgcttc ccaaaagtgt taaaaagctg taatacgtga
     3481 gatggcaaac ctcttgaaag gaccttc
//
LOCUS       DAOQGS010000442         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15408_length_3490_cov_4.406987, whole genome shotgun sequence.
ACCESSION   DAOQGS010000442 DAOQGS010000000
VERSION     DAOQGS010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15408_length_3490_cov_4.406987"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKZ52_14340"
     CDS             <1..311
                     /locus_tag="PKZ52_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61000.1"
                     /translation="LAARYAVGGGSSGTASSAFIAVSAADFAQYKHISDYLKKHSLVT
                     ACRLLQSNGKELLFELGLRASISQFNTSLSLDQRLEPSANPEGAGSAAAPLNYRWIAN
                     "
     gene            363..1094
                     /gene="hda"
                     /locus_tag="PKZ52_14345"
     CDS             363..1094
                     /gene="hda"
                     /locus_tag="PKZ52_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNG61001.1"
                     /translation="MTTSAPVQLSLGVSLKDDATFANYFQNTQANAQALAALLQVSAN
                     QGERNLAIWGSRGCGLTHLLQAVCHRAYEGGLSVQYLPLRDLIGYAADDVCEGLEAVQ
                     LLCLDGLDEICGNRQWEQALFHLFNRINLAEHRLILASHTSIPSLPILLPDLKSRLLS
                     CVIYHIESLNDAGKEQALILRAKGRGLEMSQEVAHFILTRTVRDTNELFNLLNRLDDA
                     SLQHQRKLTIPFVKEVLGHQEAEHK"
     gene            complement(1102..1524)
                     /locus_tag="PKZ52_14350"
     CDS             complement(1102..1524)
                     /locus_tag="PKZ52_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG61002.1"
                     /translation="MADSDSMSLSFHINFNDQCQAAFEFYAQVLNGQIGTMLQFKDSP
                     AAASVAPQRQSKIVHANINIEGVEIAGADVEASQYTAPTGFFILLGFASELQVEAVFA
                     RLAQGGEVIMAPQQTFWSACYAIVIDRFGVPWKLNCGN"
     gene            complement(1524..2483)
                     /locus_tag="PKZ52_14355"
     CDS             complement(1524..2483)
                     /locus_tag="PKZ52_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNG61003.1"
                     /translation="MNTKVVYSSHYDFGGWGIDKLHPFDGKKFSKAWGVVQSSVPDAQ
                     AYVLEPESPADDDLLRLVHSNEYLKSLDNSAVIAAVIEIGMARLLPISVLKNYLLTPI
                     RWATQGTLLATRYALTHNVMVMNLGGGFHHAFAEKGEGFCFFADAALAIKKMRAEGLL
                     GATDTVAMIDLDAHRGNGFESFFKDDAAVAIFDMYNFQVYPGLHGGDVDDFPFMVPLR
                     IGTIGAEYLGALREELPGFLASLKSPKIIFYNAGTDILEDDALGGLAVEFDAVIERDK
                     YVLTELRKLDVPVVVMTSGGYSSDSYKLVGECAKSLLQLNNSN"
     gene            complement(2480..3043)
                     /locus_tag="PKZ52_14360"
     CDS             complement(2480..3043)
                     /locus_tag="PKZ52_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61004.1"
                     /translation="MQKVSKPVKLYVWAMTDTNPGHVSLEIDNTYISYWPTGEDSETK
                     DRIKSPPGKGNLKDFKFGFCHQVSFPSSYRTDRRLERKDADSVLIINGLDAELMCQRW
                     EAFKAKPKRYNMKSYNCSTVVASFLEIGSGVAYGKTPCIKIDHYEADPIKRLFYKLRF
                     MGNSITMWTPNDVHQYALQIKSSKGFS"
     gene            complement(3086..3466)
                     /locus_tag="PKZ52_14365"
     CDS             complement(3086..3466)
                     /locus_tag="PKZ52_14365"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61005.1"
                     /translation="MDWPGKRDCLRLIQTPSRATLKPCGAESVDFDTTQNLFIEGDNL
                     EALKLLQKSYCGKVKIIYIDPLETDKKVYLIRETKSTHDSDKRRLEENLKILCGQAHF
                     KELNGVVYAVATSVAQVLSIDPLA"
BASE COUNT          913 a          943 c          794 g          840 t
ORIGIN      
        1 ttctcgccgc ccgctacgcc gtaggcggcg gcagtagcgg caccgccagc agcgcgttta
       61 tcgccgtgag cgctgcggat tttgcccagt acaaacacat cagcgattat ttgaaaaaac
      121 acagcctcgt caccgcctgc cgcctgctgc aaagcaacgg taaggagtta ctgtttgaac
      181 tcggcctgcg cgccagcatc agccaattta ataccagttt aagcctggac caacgcttag
      241 aacccagcgc caatccggaa ggggctggct ctgcggcggc gccgttaaat taccgctgga
      301 ttgccaacta aggcgtatat ggagtaaaaa tgcgctatct atccgtttag ggttatttcg
      361 ccgtgacaac gtccgcaccg gttcagctaa gccttggcgt atcgctcaaa gacgatgcca
      421 ccttcgccaa ttatttccaa aatacccagg ccaacgccca ggccctggcg gcgctcttgc
      481 aagtcagcgc caatcaaggc gagcgcaacc tggccatttg gggttcccgc ggttgcggcc
      541 taacccactt actgcaggcg gtttgccacc gcgcctacga aggcggcctt tccgtgcagt
      601 atttacccct gcgggattta attggctacg ccgccgacga cgtgtgtgaa ggattagaag
      661 cggtgcaatt actctgtttg gatgggctgg atgaaatctg cggcaaccgc cagtgggagc
      721 aggcgctatt tcatttattc aaccgcataa atcttgccga acaccgctta atcctcgcct
      781 cccacaccag tattcccagc ctgccgattt tattgccgga tttaaaatcc cgcctgctca
      841 gctgcgtgat ttaccacatt gaaagcctca acgacgcagg caaagaacag gcgttaattc
      901 tgcgcgctaa agggcgcggg ctggaaatga gccaggaagt ggcgcacttt atcctcaccc
      961 gcacggtgcg cgacactaat gaattattta atttattaaa ccgcctagat gatgcctcct
     1021 tgcagcacca gcgcaaatta accattccgt ttgtaaaaga agtgctcggc catcaggagg
     1081 ctgagcacaa ataaatgccg cttaattacc gcagttaagt ttccaaggca ctccaaagcg
     1141 atcgatcaca atagcgtaac aggcggacca aaaggtctgc tggggcgcca taattacctc
     1201 ccctccctgc gctaaacggg caaaaacagc ctccacctgc agctctgatg caaaccccag
     1261 caaaataaaa aaccccgttg gcgccgtgta ttggctcgcc tccacatccg ccccggcaat
     1321 ctcaactcct tcgatattaa tattggcgtg gacaattttg ctttgccgct ggggcgctac
     1381 gctagccgct gcaggagaat ctttaaactg cagcatggtg ccgatttggc cgtttaacac
     1441 ttgcgcgtag aattcaaaag cggcttggca ttggtcgttg aaattgatgt ggaaggagag
     1501 gctcatggag tcagaatctg ccattaattt gaattattaa gctgtaagag agatttcgca
     1561 cactcaccaa ctaatttata cgagtctgag ctgtagccgc cgctggtcat aacaaccaca
     1621 ggcacatcga gcttgcgcaa ttcggtcagc acatatttat cccgctcaat aaccgcatca
     1681 aactccactg ccaagccccc caaggcatca tcctctaaaa tatccgttcc tgcgttataa
     1741 aagataattt taggcgactt aaggctcgcc agaaaccccg gcaactcttc cctcaaggcc
     1801 cctaaatatt cagcgccaat agtgcctatt ctaagcggaa ccataaaggg gaaatcatcc
     1861 acatctcccc catgcagccc cggatatact tgaaaattgt acatatcaaa aatagctacc
     1921 gccgcgtcat ccttaaaaaa ggattcaaag ccattgcccc tgtgcgcatc caaatcaatc
     1981 atggcaaccg tatctgtagc acctagcaat ccttctgcgc gcattttttt aatagccagg
     2041 gccgcatcgg caaaaaaaca aaaaccctcg cctttctctg caaaagcgtg atgaaagcca
     2101 ccgcccaaat tcatgaccat tacgttgtga gtaagggcgt aacgagtggc taacaatgtg
     2161 ccctgggtag cccaacgaat aggcgttaac aggtagtttt tgagcactga aatcggcaga
     2221 aggcgcgcca tgcctatctc aataaccgca gcgatcacgg ctgagttatc aagggatttt
     2281 aaatactcat tgctatgaac cagccgcaac agatcatcat cagcgggtga ctcgggctca
     2341 agtacataag cctgcgcatc aggcacagaa gattggacta caccccacgc cttactaaat
     2401 tttttaccgt caaacggatg taatttatca atgccccagc ctccgaagtc ataatggctg
     2461 gagtaaacaa ctttagtgtt catgaaaacc ccttggagga cttaatctgc aacgcgtact
     2521 gatgcacatc attgggtgtc cacatggtta tggagttacc cataaagcgt aatttataga
     2581 acaatcgttt aatggggtca gcctcgtaat ggtcaatctt aatgcacggg gttttaccat
     2641 aggcaacgcc ggagccaatt tccaaaaaag aggcgacaac ggtcgagcaa ttataacttt
     2701 tcatgttgta gcgtttcggc ttggctttaa acgcctccca acgctggcac attaattctg
     2761 catccagccc attaataatt aatacactat cggcatcctt gcgctccaag cgccgatcgg
     2821 ttctataact gcttggaaaa gagacctggt ggcagaagcc aaacttaaaa tctttgagat
     2881 tacccttgcc gggcggactt ttaatgcgat ccttggtttc ggagtcctcg ccagttggcc
     2941 aataactgat gtaggtatta tcaatctcta gggaaacatg cccaggatta gtatcggtca
     3001 tggcccacac gtagagtttt acgggtttgc ttaccttttg catagaaaca acctcctcat
     3061 tgttgaaaca aataaataat ggtaattacg cgaggggatc gatggataac acctgggcaa
     3121 ccgaggtcgc cactgcgtaa acaacaccgt tcagctcttt aaaatgggcc tgcccacaga
     3181 gaattttcag gttctcttct aagcggcgtt tatcgctatc gtgggtcgat ttagtctcgc
     3241 gaattaaata gaccttttta tccgtctcca gcgggtctat ataaattatt tttaccttgc
     3301 cgcagtagct tttttgcaaa agctttaaag cctctagatt atcgccttca ataaacaggt
     3361 tttgcgtggt gtcaaaatcc accgattctg ccccgcaggg ctttaaggtg gcgcggctgg
     3421 gggtttgaat gaggcgcaaa caatcccgct tgcccggcca gtccatccct tagcgctccg
     3481 ggcggttatc
//
LOCUS       DAOQGS010000443         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15414_length_3489_cov_5.108037, whole genome shotgun sequence.
ACCESSION   DAOQGS010000443 DAOQGS010000000
VERSION     DAOQGS010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15414_length_3489_cov_5.108037"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..303)
                     /locus_tag="PKZ52_14370"
     CDS             complement(40..303)
                     /locus_tag="PKZ52_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61006.1"
                     /translation="MLSPSNGTLLPHDIQQLQSLLQTETEQKERALAKVVLLEEEIRA
                     LKLVLATHNGAAPQAQNTSAQFNGELILVVDDQPLNLDLIGSS"
     gene            complement(413..649)
                     /locus_tag="PKZ52_14375"
     CDS             complement(413..649)
                     /locus_tag="PKZ52_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61007.1"
                     /translation="MASLLKAKNLTLQMAIALSVKELTHPTGKRPNIINPAVFSAGLI
                     KAARMPHSAVQAAASCVKTRKSTHFRKFNAATAK"
     gene            939..3020
                     /locus_tag="PKZ52_14380"
     CDS             939..3020
                     /locus_tag="PKZ52_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNG61008.1"
                     /translation="MPLFINTNVSSINAQRQMVKAGAEMDKAMSRLSSGMRINSAKDD
                     AAGLAISNRQTSQIRGLDQAVRNANDGVSLIQTAEGALGESTNILQRMRELAVQSSNG
                     IYSDADRSTLDAEVKQLTKELDRIAKSTSFNGQNILDGSVKKVALQVGTEAGQTIDLK
                     LQSVDAKTLGMGSLTSDLMGDEITATFDSQVSLTNGDLLINGQSVIQDTDFVGSTDTV
                     ESLLNKINANVKGVTASMVVELNASNVGTGVADSAGTTITLTKLNGATSVFNVADTKN
                     LQEFADKVNSVSGGAISATIDDKGLLHLMAKDAQTLAVGAAGNTGIGDTSDRGRIVLT
                     SDNGDPITIERGATGSLDKLDRLGFREIRKPGTIEGAALVNDSSGAHEALAVGELTIN
                     GVVISETDTNTLQGKINNINKVSDQTGVIAKASSSVTIDFTGYVSSNVTSTLDNIVMN
                     GTIYDLGLAVSATNTDIATAINTFKEESGITARVAGRNIILESDRGAIQLGIVQSGAV
                     NLFGTSTGLQTITQAMVDSTAAFVTSSTTLASSGTMSYQAGARIKLESINGNPISVEL
                     SNDSDAARLGLKETNNLSGGSFGQSISSISIDTAANASKALKVIDNALNTVNDIRSQL
                     GAVNNRLDFTVSNLMNVSENTSAARSRITDADFAAESANLSRSQVLAQASQAMLAQAN
                     AKPQQVLSLLR"
     gene            3126..>3489
                     /locus_tag="PKZ52_14385"
     CDS             3126..>3489
                     /locus_tag="PKZ52_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNG61009.1"
                     /translation="MNEVSQVRLVPQVALSSSVAKSDSNRKLTDKNLAAALPAEREAV
                     ISSTQAVHSDEQKGKQLKDALGTMQDFVQNVKRELQFSVDKDLGRTVVKVVDSESGDL
                     IRQIPEEIFLELARKVKEH"
BASE COUNT          951 a          776 c          835 g          927 t
ORIGIN      
        1 aggtcatttt gccatctggc gcgggggttt tacacaattc tacgaagagc caattaaatc
       61 aaggttaagc ggctgatcat caaccactaa aattaattca ccgttaaatt gtgcgctagt
      121 gttctgtgct tgcggcgccg cgccattgtg tgtggctaga accagtttaa gtgcgcgaat
      181 ttcttcttcc aacaatacca ccttggctag ggcgcgctct ttttgctctg tttctgtttg
      241 caataggctt tgcaactgct gtatgtcgtg cggcagaagc gttccatttg atggcgagag
      301 cataacaata atggcctgtg tatttaataa aagtgctcag agtgaaaaac acgctttaat
      361 tcgcggctcc ataaccattt attaacccga cggcaaaata gatcgtcctg atctattttg
      421 ccgtcgctgc gttaaactta cgaaaatgcg tggattttct tgttttaacg cagctcgcgg
      481 cggcttgcac cgccgagtgc ggcatccttg ccgcctttat taacccggca ctaaataccg
      541 ccgggttaat aatattcggt cgtttaccag ttggatgcgt taattccttg acgcttaagg
      601 ctattgccat ttgtaaagtc aaattcttgg cctttaataa cgaagccaaa gcctcgcttg
      661 agtatagttg gctcgcagcc tcttataggg gcaattagtg ctgacctctt taattgctag
      721 gtttttatag ccaaaacacc tatgagcgtg gtttttaggt tgttctgctt taagcactat
      781 ggattagggc tgcagattat ttttttagta gaaaagtact aaagatattt tgagggtggc
      841 cgatagattg ggtagaagcg ctcaactact gagctttcgg attgccgctg gcgttaaggc
      901 ttggtcgcaa tcataagacc aacgtgttag gaggctctat gccactgttt attaatacca
      961 acgtttcatc cataaacgct cagcgccaga tggttaaagc cggcgctgaa atggacaagg
     1021 ctatgtcccg tttgtcatcc ggtatgagga ttaactcagc taaggatgac gcagcaggcc
     1081 ttgctatttc taaccgccaa acttcgcaaa ttcgcggcct tgatcaagcg gtgcgcaacg
     1141 ctaacgacgg tgtgtccttg attcaaaccg ctgaaggtgc cttgggcgaa agtaccaata
     1201 ttctgcaacg tatgcgcgaa ttggcggtgc aatcctccaa cggtatttac agcgacgccg
     1261 accgctccac cctggatgcc gaggtgaaac agctcaccaa agaattagat cgtattgcta
     1321 aatctacttc ttttaacggt caaaatattc ttgatggctc agtcaaaaaa gttgccctgc
     1381 aagtgggaac cgaagcaggc cagactattg accttaagct gcaatcagtg gatgccaaaa
     1441 ccctcggaat gggttcattg acttcagact taatgggcga tgaaattacc gctacattcg
     1501 actctcaagt ttcgttaacc aatggcgatt tattaattaa tggccagtcc gtgattcaag
     1561 atacagattt tgtcggtagc actgacacag tcgaaagtct gctaaataaa attaacgcta
     1621 atgtgaaagg tgttactgcc tctatggtgg tggaattaaa tgcctccaac gtaggcacag
     1681 gtgttgccga cagcgcaggc accaccatta ctttaaccaa actcaatggt gcaacctccg
     1741 tatttaacgt ggcggatacc aaaaatttac aagaatttgc cgacaaagtt aattcagtat
     1801 ccggcggtgc gattagcgcc accattgatg ataaaggctt attgcattta atggcaaaag
     1861 acgcacaaac cctagccgtg ggtgcggccg gcaacacggg tattggcgat acctctgatc
     1921 gtggtcgaat tgtactgacc tctgataacg gcgacccaat tactattgag cggggggcaa
     1981 ccggttcgct ggataaattg gaccgcctag gatttagaga gatacgtaag cccggtacta
     2041 tagaaggcgc cgccttggtt aatgactcca gtggtgcgca tgaagctctg gcggtcggcg
     2101 aattaactat taatggcgtg gtgatatcgg agaccgatac caatacccta caaggaaaaa
     2161 tcaataatat aaacaaggta tccgatcaaa caggcgtcat cgctaaagct tcatcttcag
     2221 tgacaattga ttttactggc tatgtaagtt caaacgtaac atctacccta gacaatatag
     2281 taatgaacgg tactatttac gatttgggcc tggccgttag cgcaaccaat accgatatag
     2341 ctaccgcaat taataccttt aaagaggagt cgggtattac tgcacgagtc gccggtcgca
     2401 atattatttt ggagtccgat cgcggtgcta tccaattggg tatagtgcaa agtggtgctg
     2461 tgaatttgtt tggaacctcc acagggttgc aaactattac acaagcaatg gtggattcta
     2521 ctgcagcttt tgtaacctca agtaccacct tggcttcgtc aggtactatg agttatcaag
     2581 ctggcgcgcg cattaaactg gaaagtatta atggcaaccc aattagcgtt gagctgagca
     2641 atgactctga tgctgctcgc ttaggtttaa aagaaaccaa taacctaagt ggaggctctt
     2701 tcggccaatc aatatcatcc atcagtattg ataccgccgc taatgcctct aaagcattaa
     2761 aagtgattga taatgcttta aacacagtga acgacatccg ctcgcaactg ggtgctgtga
     2821 acaaccggct tgattttacc gtgtcgaact taatgaacgt atcggaaaat acttcagctg
     2881 cacgttcgcg cattaccgat gcagactttg cggcagaaag tgcgaaccta agccgctcgc
     2941 aagtattggc gcaagcctcg caagctatgt tggcgcaagc caatgcaaaa ccgcaacaag
     3001 tgttgtcact cctgcgttaa tggagtgatt aatgaattaa agcgtctatt attaactagg
     3061 cgttttcaat tctcgaataa caccggggat tttctccccg gtttttggcg ttacggaggt
     3121 tttatatgaa tgaagtaagc caagtgagac tggtgccgca agtggcgctt tcatcgtcgg
     3181 tggccaagtc agactccaat cggaaattga cggataaaaa cctggccgca gctctaccgg
     3241 ctgagcgcga agccgtgatt agctcaactc aggctgtgca ctcagatgag caaaagggca
     3301 aacagctaaa agatgcgctc ggcactatgc aagactttgt gcaaaacgtg aaacgggaat
     3361 tgcagtttag cgtggacaaa gacttaggcc gcacggttgt taaagtggtg gattccgagt
     3421 ccggtgatct aattcggcaa attccagagg aaatttttct ggagttggca agaaaagtga
     3481 aagagcacg
//
LOCUS       DAOQGS010000444         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15464_length_3483_cov_4.202450, whole genome shotgun sequence.
ACCESSION   DAOQGS010000444 DAOQGS010000000
VERSION     DAOQGS010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15464_length_3483_cov_4.202450"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKZ52_14390"
     CDS             <1..237
                     /locus_tag="PKZ52_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HNG61010.1"
                     /translation="GIGAALTISQLIEGIEALLGIQIFDPEVYFVSYFPSVWQWQDTL
                     KVVTGAAFTCVIASIYPAWRAAQIAPAEALRYNI"
     gene            271..978
                     /locus_tag="PKZ52_14395"
     CDS             271..978
                     /locus_tag="PKZ52_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG61011.1"
                     /translation="MTAPVLECADLSKTYAQGELVVDVLKAVNFSVQAGQQVAIVGAS
                     GSGKSTLLNLLGGLDRPDTGWVKINGQAFSSLSENARGQMRNRHLGFVYQFHHLLGEF
                     TALENVCIPLLIAGVAQKTAQAQARDLLGQVGLLERIAHKPAELSGGERQRVAIARAL
                     ANKPACVLMDEPTGNLDGKSAAAVQALMHRLSAELATAFVVVTHDLRFANTMNRVWQL
                     NDGSLNTIEVNTTGAAL"
     gene            975..2204
                     /locus_tag="PKZ52_14400"
     CDS             975..2204
                     /locus_tag="PKZ52_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNG61012.1"
                     /translation="MNLPLSIGLRYAGTKKHNALLAFLARISIVGLALGVGLLITVLS
                     VMNGFDRELKQKILALMPQGIIYNHDGISDWQVLKQRLMQQPGIVAASPFVELNGLVS
                     KNTAAEPVILYGIRPDEEIAVSHLHTYINAAVMQQLSEGQRGLVLGKPLADKLQVQVG
                     EPIMLIVPDTQSTQGAAKLDYFTVLSILETKTELDQHLAITSLEAAAQLNGRSQEVSG
                     MRVKVADLFEARSIVWHAVMGLGEGYYGTSWMTTHGNLYTAIQMSKNLVGLLMSLIVA
                     IAAFNVVSTLVMVVVEKQGDIAILRTLGMSTHQIMQIFMVQGTLIGFIGTGLGVLGGL
                     GLSLCVESLVRGIEALFHVQFLKTDVYPLTYLPAEIVWLDVAKVAGTALLMSFLATLY
                     PSYKAARIQPAEALRYE"
     gene            complement(2219..2992)
                     /locus_tag="PKZ52_14405"
     CDS             complement(2219..2992)
                     /locus_tag="PKZ52_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG61013.1"
                     /translation="MSEHIILFLVSLLANFLSALAGGGAGLLQLPALIFLGLPFATAM
                     ATHKIATVTLGMGASIKHLKAGTLDLRFASFILVAGLPGVLIGAVGAVHLPETIAKTC
                     LGVLTISLGIYSWSNKSLGQTSLAANRHRQGLWLGGAVIFIIGLLNGALASGTGLFVT
                     LWLIRWFGLDYKQAVAYTMILVGLFWNGSGAIAVNSLSPAQWNWLPALIAGSLLGGYA
                     GTHIAILKGNGFIKKMFEVLTLASGLWLLWNAWAQPFHT"
BASE COUNT          886 a          821 c          890 g          886 t
ORIGIN      
        1 ggcataggcg cggccctcac tatttcacaa ttaatagagg gcattgaagc cctgctcggt
       61 attcaaatat tcgaccctga agtgtatttc gtcagttatt ttccctccgt ctggcagtgg
      121 caggatacct taaaagttgt cacaggcgcg gcttttacct gtgtgatcgc cagtatttat
      181 cctgcgtggc gcgccgctca aatcgccccg gccgaagcgc tgcggtataa catttaaatc
      241 gcaaccatta atgactatct acgaggtgtt atgaccgcgc cggttttaga atgcgctgat
      301 ttatccaaaa cctacgccca aggggagttg gtggtggatg tattaaaagc ggtgaatttt
      361 tccgtacaag ctggccagca ggtggccata gtgggtgcat ccggctccgg taaatccacc
      421 ctattaaatt tattaggcgg cttggatcgg ccggataccg gctgggtcaa aattaacggc
      481 caggcgtttt cttccttatc ggaaaacgcc cgtggccaga tgcgcaaccg ccatttgggg
      541 tttgtgtatc agtttcacca tttgttaggc gaatttaccg ccttggaaaa cgtctgtatt
      601 ccgctgttaa ttgccggtgt agcgcaaaaa acggcgcaag cacaagcgcg ggatttatta
      661 ggtcaggtgg gtttattaga gcgcatcgcc cataaaccgg cggaattatc cggcggcgag
      721 cgtcagcgcg tcgccattgc cagggcactt gccaataaac ccgcctgtgt attaatggat
      781 gaacccaccg gcaatttgga cggtaaaagt gctgcagcgg tgcaagcgct gatgcatcgc
      841 ttaagcgccg agcttgccac cgcttttgtg gtggtgaccc acgatttgcg ttttgccaac
      901 accatgaatc gcgtatggca attaaatgat ggcagcctta atactattga ggtgaatact
      961 actggggcag ccctgtgaat ttgccgctaa gtattgggct gcgctacgcg ggcacgaaaa
     1021 aacacaatgc gctgctggcg tttttagccc gtatttccat tgtgggcctg gccttagggg
     1081 tgggtttatt aattacggtt ttatccgtga tgaacgggtt tgatcgcgaa ctcaaacaaa
     1141 aaattctcgc cttaatgcca caaggcatta tttacaacca cgacggtatt agtgattggc
     1201 aggtattaaa acagcgttta atgcaacagc cgggcattgt ggcggcttca ccctttgtgg
     1261 aattaaatgg tttggtgagt aaaaacaccg ccgccgaacc ggtaattctt tacggtattc
     1321 gcccggatga agaaattgcc gtcagccatt tgcacactta tatcaacgcc gcggtaatgc
     1381 aacaattaag tgaaggccag cgcggcttgg tattgggtaa acccctggcg gataaattac
     1441 aggtgcaggt gggcgagccg attatgttga tagtgccgga tacccaaagc acacaggggg
     1501 ctgcgaagct ggattatttt accgtgctga gcattttaga aaccaaaact gagttggatc
     1561 aacacttggc aatcacctcc ttggaagccg ccgcgcaatt aaacggccgc agccaggaag
     1621 tatccggtat gcgggtaaaa gtggctgatt tatttgaagc gcgcagcatt gtttggcacg
     1681 ccgtgatggg gttgggggag ggttattacg gcaccagttg gatgaccacc cacggcaatt
     1741 tatacaccgc tattcaaatg tcaaaaaatc tcgtgggatt gttaatgtcg ttaatcgtcg
     1801 ccatcgccgc ttttaatgtg gtgagtacat tggtaatggt ggtggtggaa aaacaaggcg
     1861 acattgcaat tctgcgcacc ctgggcatga gcacccatca aatcatgcag atatttatgg
     1921 tgcagggcac cttaattggt tttattggca ccggcctagg tgtgctgggc ggtttgggat
     1981 tatccctgtg tgtggaatcc ttagtgcgcg gcattgaggc gttatttcat gtgcagtttt
     2041 taaaaaccga tgtctatccg ctcacctatt taccggcgga aattgtttgg ttggatgtgg
     2101 caaaagtggc aggcacggct ttactgatga gttttttggc aaccctatac cccagttata
     2161 aagctgcccg tattcaaccg gcggaagctc tgcgctatga ataaatatgc cccggtcttt
     2221 aggtgtggaa tggttgtgcc caggcattcc acaataacca taatccgctt gccaaggtga
     2281 gcacttcaaa cattttttta ataaagccat tgccctttaa aattgcgata tgggtgcccg
     2341 cataacctcc cagtagggag cctgcaatta atgctggcag ccaattccac tgggcggggc
     2401 tcaaggaatt taccgctatt gccccagagc cattccaaaa taaacccacc agaatcatgg
     2461 tgtaggcaac cgcctgttta taatccaaac caaaccagcg aataagccaa agtgtaacga
     2521 ataagcccgt gccagaggct agtgcgccgt ttaaaaggcc aataataaaa atcaccgcgc
     2581 cgccgagcca taaaccctgc ctgtggcggt tagcggcaag tgatgtttgg ccgagtgatt
     2641 tattgctcca agaataaata cctaaactaa tggtgagcac acctaaacag gttttggcga
     2701 tggtttcagg taaatgtact gcccccaccg cgccgattaa tacaccgggc aaacccgcca
     2761 ctaaaataaa gctggcaaaa cgcagatcta atgtacctgc ttttaaatgc ttaatacttg
     2821 cgcccatgcc gagggttacc gtggcaattt tatgggtggc catggcggtg gcaaagggca
     2881 ggcctaaaaa aatcagtgcc ggtaactgca acaaacccgc gccgcctcca gctagagccg
     2941 ataaaaaatt ggccaaaagg ctgactaaaa ataaaatgat atgttcggac attagttggg
     3001 cttaacaata aatgaggtga aggtttggga tggaggagga aacataattt gcccccttaa
     3061 aaagtacatc gtcttacaat aggagtatcg attttttact gggcgctttg ttttgtaaat
     3121 atcacatggg tgttttagtg cgttgagccc tcaatgctca cccgcagtga tgactacagg
     3181 ttagtgcaga cacgccgtaa atacatccgt gtaggctcgt caaaaaggcc cctctttttg
     3241 acggtctgca ataacctgta gtcaccactg ctacaccgcg aatcgattat gccgcatggc
     3301 aaggtcctcc aaaataagtc gcccgcgcgg atagggtaat aaggtgtgct gaatagttac
     3361 ttttttggca ctttgctcct taccaccccc cccttaacaa ggggcggagc aaaaccttcc
     3421 agccgaggca ttttgcacgt gctttcggta tttttgcccc ctccccttaa caaggggagg
     3481 gct
//
LOCUS       DAOQGS010000445         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15477_length_3482_cov_4.198132, whole genome shotgun sequence.
ACCESSION   DAOQGS010000445 DAOQGS010000000
VERSION     DAOQGS010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15477_length_3482_cov_4.198132"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1482
                     /locus_tag="PKZ52_14410"
     CDS             118..1482
                     /locus_tag="PKZ52_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNG61014.1"
                     /translation="MNNPTLKAPAQLTGHWLWGSARELAAAPHEFIAHAPSAFNGLVS
                     FRVLHKRIIAVNDPNIVRQIMVTQQQRYRRSYHYTNPVLGQGLLSTDGALWQKRRRQV
                     QPAFRRETMLHLVRMANSTVDKLFAHWDAAKAASQPLELLSEMQKFALSVNSQALLSL
                     DVSWDAAEKFGIAVSDALKLLRKRNNAPVRFLAWLPTPLNRQLTHTRTILEEHIAPII
                     KARRSQSADAPQKNALQSDILTALLDARDPETGDALSDQEILDETKTLFAAGFETTAT
                     ALTWAIYLLVHNPEQAAHWYAEVDSVLAGRQPQWEDLPNLPFTANMINEVLRLYPPVY
                     NIARECIEDDEINGMHIKKGSVLVMSILGIHRDERWWPEPLAFLPERFNASWHKHAFL
                     PFATGRHLCVGNHFALTEMAVALIRLAQRYRVELIDTSPVGMAAQITLVPNRPLHFKL
                     LARP"
     gene            1479..2027
                     /locus_tag="PKZ52_14415"
     CDS             1479..2027
                     /locus_tag="PKZ52_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61015.1"
                     /translation="MRPFTAQLLDLGDDTAVVQYEQAFYQAFYTLTHNRLIRTLWHWD
                     LDQQRLATRIGYEHQRIYVGRDMQGEIAMAIAVNTELQLYQSGAFGFPKPAHTTGQCE
                     LLAFFAINNHHLQHTLHFWRSCFKDLSACGFHTGFASSAARLVPVYSRAGGEILDKTV
                     LEGEQRFHWKFDLNRQWLKGQN"
     gene            complement(2109..3029)
                     /locus_tag="PKZ52_14420"
     CDS             complement(2109..3029)
                     /locus_tag="PKZ52_14420"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HNG61016.1"
                     /translation="MKFRLKHLLRIYCGFLRQTPPLFSGHVKHAIWGAAEQAAELPVI
                     PNIIWLYWDAEQITSITAELCINLIKSLHPVFDVRLLNKITISDYLPDFPKELADKAP
                     NFISDMVRLMLIEKYGGIYLDATVLLSKPIDWALKFQQDDRSEAVLYYTDENTVDEEF
                     PMVESWFIVAPPNSKFIRAWREEYQNSITCADIDRYWRGCELLPQSKFPLRLPYYLCY
                     WAAQRVLRKNQDYRLTLLRAEDDAFSYGLGFKKKWDEVAMADLMLFNKKPAPCPNLVK
                     LIRYDRVRLDYYIQRKFYKKDSWLGELIAE"
BASE COUNT          958 a          890 c          760 g          874 t
ORIGIN      
        1 attaattgca acaggcaggc cattaggtgc ctttttataa tcgcccggaa acaatttaat
       61 tgtatgcaca tacgccagtt tcgtatcaca ccgtcttcct gcgagtaaca gcgccttatg
      121 aataatccca cactaaaagc ccccgcccaa ttaaccggcc attggttgtg gggcagtgca
      181 cgggaattag ccgctgcgcc ccatgaattt attgcccatg cgccttcagc atttaatggc
      241 ttggtgagct ttcgcgtatt acacaaacgc atcattgccg taaacgatcc gaatattgtg
      301 cgccagataa tggtgacgca gcagcagcgt tacaggcgca gttatcacta taccaatccg
      361 gtactggggc aaggtttact cagcacagac ggcgccctat ggcaaaaacg ccgccgccag
      421 gtgcagccag cctttcgccg cgaaaccatg ctgcatttag tgcgtatggc caattccacc
      481 gtagataaat tattcgccca ttgggacgct gccaaagctg catcgcagcc cttggaatta
      541 ctcagtgaaa tgcaaaaatt tgcactgtcg gtaaattctc aggcgctgct atcgttagat
      601 gtaagctggg atgccgccga gaaatttggt atagccgtaa gcgatgcctt aaaactcttg
      661 cgcaaacgca ataatgcccc agtgcgcttt ctcgcttggc tacccacccc cctaaatcgg
      721 cagttaacgc acacacgcac tattttggaa gaacatattg cccccatcat taaagcgcgc
      781 agatcccaga gtgcagatgc tccacaaaaa aatgcactgc agagcgatat attaacggct
      841 ctattagacg ctcgcgaccc tgaaactggc gacgcactga gcgatcagga aattctggac
      901 gaaaccaaaa ctctatttgc agctggtttt gaaaccacgg caacggcact tacctgggcc
      961 atttatttat tggtacacaa tcctgagcaa gcagcgcact ggtatgcaga agtggatagt
     1021 gtattggcag gaagacaacc ccaatgggag gacctaccga acctgccgtt taccgccaat
     1081 atgattaatg aagtgttgcg gctttaccca ccggtttaca acattgcccg cgagtgtatt
     1141 gaagacgatg aaataaatgg catgcacatt aaaaaaggca gcgtactggt aatgtctatt
     1201 ttaggcatcc accgcgacga gcgttggtgg cccgaaccct tggcattttt gcccgagcgt
     1261 tttaacgcca gctggcataa acacgctttt ttaccttttg ccactgggcg gcatttatgt
     1321 gtgggcaatc attttgcttt aaccgaaatg gcggtggcat taattcgcct agcgcagcgc
     1381 tatagagtgg aattaattga tacctcgccg gttggcatgg cggcacaaat caccctggtg
     1441 cccaaccgcc cactgcactt taaattgctg gcccgcccat gaggcctttc accgcacaat
     1501 tattagatct tggcgatgac acagccgttg tgcagtacga gcaggcgttt tatcaggctt
     1561 tttatacact cacgcacaac cgattaatac gcaccctatg gcactgggat ttagaccagc
     1621 agcggctggc aacccggatt ggctatgaac accagcgtat atacgtgggc cgcgatatgc
     1681 agggtgaaat cgccatggcc attgcagtta atacagaatt acaactgtat caatccggcg
     1741 catttggttt tcctaaaccc gcccatacaa ccgggcagtg tgaattatta gccttttttg
     1801 ctattaataa tcaccatctg caacacacct tacatttttg gcgcagctgt tttaaagatt
     1861 tatccgcctg cggcttccac accggctttg cctccagtgc agctcgtttg gtgccagttt
     1921 acagccgtgc gggcggtgaa atattagata agacggtact agagggtgag caacgctttc
     1981 attggaaatt tgatctgaac cgccaatggt taaaaggcca gaattagttt caacccggtg
     2041 gaattttatg ccgagttagt agcatacacg cttggcagat tgagaataaa attgcgggct
     2101 ccagccaatt actcagcgat tagctcgccc agccaagagt cctttttgta gaatttgcgc
     2161 tgtatataat agtccaatcg aactcggtcg tagcggataa gttttaccag gttagggcac
     2221 ggggcgggct ttttgttaaa caacatcagg tcggccatag ctacctcatc ccatttcttt
     2281 ttaaagccga gaccgtaact gaacgcgtca tcttcagcgc gcagcagcgt taggcgatag
     2341 tcttggtttt ttcgcaacac acgttgagcg gcccaataac acaaataata aggcagcctt
     2401 aaggggaatt ttgactgggg caacaattcg caacctcgcc aatatctatc aatgtctgcg
     2461 catgtgatac tgttttgata ttcctctcgc caagcgcgga tgaacttgga attaggcggc
     2521 gccacaataa accaactttc aaccattgga aattcctcat ccactgtatt ttcatcggta
     2581 taatacaaca cagcttccga gcgatcgtcc tgctgaaatt ttaaagccca atcgatgggt
     2641 tttgaaagta acacggtagc atccaggtaa attccaccat atttctcaat caacataagc
     2701 cgaaccatgt ctgaaataaa attaggagct ttgtcggcca gctcctttgg gaaatccggc
     2761 agataatcag aaatagttat tttattgagc aaccgcacat caaacaccgg atgcaagctt
     2821 tttattaggt tgatacaaag ctcagccgta attgaagtta tttgttccgc atcccaatac
     2881 aaccaaataa tatttggaat aacaggcaat tccgcagcct gctcagctgc accccaaata
     2941 gcgtgtttga catgcccact aaaaagcggc ggagtttgcc ttaggaaacc acagtagatt
     3001 ctgagcagat gcttgagcct gaatttcacg atgttttaac cagtgtcaaa ttaataaatt
     3061 atttatatcg ccgctttaac gcccttgcta tcagtacagc gtgctgctta ataatatcgt
     3121 cacctatgcg tccaacacgg cagataggta tttattttaa catccatcat ccctaggccg
     3181 ttaaatattt taactctaat ccatataccc tacccacacc aaacagcggc accaactcac
     3241 acctaaatac ctaacagcct atagaaaaat acctaaacca aggagcccgg gcaacttgcc
     3301 gcttgcgcag ctgttattaa cccggcggca aagatgccaa ccctgcggcc taagccgccg
     3361 gaagctacgc taaaaaagaa aacccgccca ttttcgaccg ttttagctca gtgaaggcaa
     3421 aaatagatca ggacgatcta ttttgccgcc aggttaataa aacggcagaa atcacctgca
     3481 cc
//
LOCUS       DAOQGS010000446         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15637_length_3458_cov_3.989715, whole genome shotgun sequence.
ACCESSION   DAOQGS010000446 DAOQGS010000000
VERSION     DAOQGS010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15637_length_3458_cov_3.989715"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..235
                     /locus_tag="PKZ52_14425"
     tRNA            151..235
                     /locus_tag="PKZ52_14425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Leu,seq:cag)
     gene            complement(351..881)
                     /locus_tag="PKZ52_14430"
     CDS             complement(351..881)
                     /locus_tag="PKZ52_14430"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNG61017.1"
                     /translation="MLKADCEIFANYNGAMNKSIYTCAAQLDALALELDRGAFFGSIL
                     GTLNHIMVADIIWLKRFANHPARFNALQIMSEFPTPKALNEILYSTLAPLLYQRRALD
                     NIIGAFVQELNDEVLASALSYKNLQGDEIKKNLGFLLLHFFNHQTHHRGQLTTLFSQL
                     GIDVGVTDLFVCIPSA"
     gene            complement(973..1401)
                     /locus_tag="PKZ52_14435"
     CDS             complement(973..1401)
                     /locus_tag="PKZ52_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNG61018.1"
                     /translation="MSERAGSCLCGAVTYRLTSEPVMARICWCRTCQHLSANGTVNII
                     VPTDALSSVGVTSEYVSIADSGNEVKRRFCPQCGCHLFANSSARPQFTVVRAGTLSDP
                     SSIKPNANIWASSAPQWACMDPALERFEQQPAPPAARPQP"
     gene            complement(1906..2715)
                     /locus_tag="PKZ52_14440"
     CDS             complement(1906..2715)
                     /locus_tag="PKZ52_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNG61019.1"
                     /translation="MALAHPQAQPLALQPVWSVEGIDTPESVLEVVNKRQNYFLVSSI
                     GGDPFVKDGVGGLVKINGRGEILDKNWLSGLNAPKGMAQFKDKVYVADIDEVVVVDIN
                     TAQVVEKIPMPGAIMLNDVAADERGTIYISDTFAARVYRLKNHKVDVYLEEVVSANGL
                     WAEPDRLLVGAANQLLAYNAAKKSKQIGSDLPLEIDGITPFKCNSYLVSSWEGQIWSI
                     SKKGEQTLLLDSVAEGINTADIMYSESAKLLFVPNFFANKVSAYKVKGSAD"
BASE COUNT          974 a          844 c          806 g          834 t
ORIGIN      
        1 ggcttgcgga tcgacaaaag atggcggttg cttcaaaggt ttagtcctgc ataataagag
       61 ttgacaagag tatacagcct atcgccacaa atagctggtg caagtgttga cacctgtagc
      121 gactatcttt atagtcgcgg cctcatttta gcccaggtgg tgaaattggt agacacgcca
      181 gcttcaggtg ttggtggcgc aagccgtgga ggttcaagtc ctctcctggg caccatacaa
      241 aaaaaggccg ctttatgcgg ccttttttat tgctcttttt tgcaattatt ttctaagcac
      301 ttgttgaaat acctaggcgg cacccttgca aggcaaattt ggcgaacaac ctaagcgcta
      361 ggaatacaca cgaataaatc cgtcacccca acatcaatac ctaattggct gaagagcgtt
      421 gttagctggc ctcgatggtg ggtttggtga ttaaaaaaat gcagcaaaag aaagcctaaa
      481 ttcttcttaa tctcgtctcc ctgcagattt ttatagctca atgcagaggc gagcacttca
      541 tcgtttaatt cctgcacaaa ggcgccgata atattatcca acgcgcgccg ctggtataac
      601 aaaggcgcga gggtagagta aagaatttca tttaacgctt taggcgtggg aaattcgctc
      661 ataatctgca gcgcattaaa gcgcgcggga tgattggcaa agcgttttaa ccagataata
      721 tccgccacca taatatggtt gagcgtaccg agtatagagc cgaaaaaagc cccccgatcc
      781 aactcaagcg ctagcgcatc cagttgcgca gcgcaggtat aaatactttt attcatggcg
      841 ccattatagt tggcaaaaat ctcacaatcg gcttttaaca tgggacgctc gcaactaatt
      901 aggtggcata acacaaaggt gtagttggct ggactataca cgcgcagtaa tgcgcgcaaa
      961 tgcctgccat ggttagggct gcgggcgggc cgcaggtggt gccggctgct gctcaaatcg
     1021 ctccaaggcc gggtccatac aggcccattg aggcgcgctg gaggcccaaa tattggcatt
     1081 gggtttgatg gaagacggat cgcttaaggt acctgcgcgc acaactgtaa actgcgggcg
     1141 cgccgaagaa ttagcaaaca aatggcatcc acattggggg caaaaacggc gtttaacctc
     1201 attgccgcta tcggcaatgc tgacatattc actggtaaca cctaccgacg ataatgcatc
     1261 tgtgggcaca ataatattca ccgttccgtt ggccgatagg tgctgacagg tgcgacacca
     1321 gcaaatgcgc gccatcacag gctcactggt taagcgatag gttacggcgc cacaaaggca
     1381 actgccagct cgttcagaca taaaggctcc tcaatatgcg ggttggtgta cgtgcagcag
     1441 gtgccaacat acctcatcca tgccgccagt ggcaatacaa ccctgcacag gttcagcggc
     1501 agtttaatgg agcgcaattg ggccgcgcgc gcggctatta ggcccgcagg aatttaaggg
     1561 atcgattgta aaacgatgta tttgcccctt gtcgcagcta ggcatttata atcacccggc
     1621 aatcaggcca gcacataaaa tttccgtgca tcttttgcca tccccataca ggcgggaata
     1681 ctgtgcagtc atcgcgggca aaagcccgcg atgactgcac aataacacga gccgccacct
     1741 gagtttggtg ttgaaaaact gtgcggactt caaagcgcca gaggacaacc tggcgcatta
     1801 acggggtgtt gaaataccta gcagcttgtt gaaatactac tttactagct ctggcgacgc
     1861 cgcctaggta tttcaacaag ctgctaggcg gcaggtgaag agaaattaat cagcgctacc
     1921 ttttacttta taagcgctga ctttattggc gaagaagtta ggcacaaata ataacttcgc
     1981 actctcactg tacataatat ctgcagtatt aattccctct gcaacagaat ctaacaatag
     2041 agtctgctca ccttttttgg aaatagacca aatctgccct tcccaggagg agaccaaata
     2101 actattgcat ttaaatgggg taataccgtc tatttctaac ggcaagtcgc tgccaatttg
     2161 tttggatttt ttcgcggcat tgtaagcaag cagttgattg gccgcgccca ccaagagtcg
     2221 atccggctca gcccagagac catttgcact taccacttcc tcaaggtaca catccacttt
     2281 gtgatttttt aagcggtaca cacgggcagc aaaagtatcc gaaatataaa tggtgccgcg
     2341 ctcatctgcc gccacatcgt tcagcataat cgccccaggc ataggtattt tttctacaac
     2401 ttgggcagta ttaatatcaa ccactactac ctcatctata tccgccacat aaaccttatc
     2461 cttaaattga gccattcctt tgggcgcgtt taaacccgac agccaatttt tatccaaaat
     2521 ttcaccgcgg ccattgattt taactaggcc gccaacacca tctttcacaa acgggtcgcc
     2581 gccaatggat gagactaaaa agtaattttg ccgcttgtta actacctcaa gtacagactc
     2641 aggagtatca ataccttcta cagaccagac tggttgcaag gctaggggtt gagcctgcgg
     2701 atgggccaat gccatccctg acccaaacgt gagcatcgca agagataagc ctgcacataa
     2761 tttttttata tagggagttt tcataggtgc ctctgaagta agagttaaac aaaatgagaa
     2821 aattagcgct aaagcaaaat cccgttgccg cccgcacaac cgaaaaaagc acacctaatt
     2881 aaatgcaggc gcacccctcg tgaagaccaa cactacaggg ttttattggc catgtatttg
     2941 ttgtgcacac accaaccagg aaccatgggc tcacctttta cggcataaag aaaggatgca
     3001 gtgatgtgta acccgacggc atttaatgcc gggttaataa tggcggtaag gatgctgcac
     3061 caaggccgca agccgccagg cttcgctaga acaggaaatt tcgcgcattt tcgaatgcgt
     3121 cagcgagagt gagagcgccg aaaggtttta gctaatgttt tttgtttgtg cctattcagc
     3181 acacctcatt actatatccg cgcgggcgac atatttagga ggacttaaac atgcggcaca
     3241 attgatacgc gctttaacag ttgagactac aggtggagta ttgacggctc accacagtaa
     3301 agcatgcatg tgatagtccc caatcagtgc gggaattacc tccaaacaca taaaaatatg
     3361 ctgcatggtt agttttgttt taaagcagaa aaaagctgca caagcgccaa aatcgatacc
     3421 gctcagcaat ttttgaaggc aaaaaaaaag cgccgaaa
//
LOCUS       DAOQGS010000447         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15655_length_3455_cov_8.695294, whole genome shotgun sequence.
ACCESSION   DAOQGS010000447 DAOQGS010000000
VERSION     DAOQGS010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15655_length_3455_cov_8.695294"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..3380)
                     /locus_tag="PKZ52_14445"
     CDS             complement(93..3380)
                     /locus_tag="PKZ52_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61020.1"
                     /translation="MEKNGIAGNKVKGVFHGGSIFVNREALQTSQDVETVILHELAHV
                     GLAKMFGQDIQAAMGSLYLAMGGDKTLRELATKYAMPLEKYQAAYASAKPAERLGFMT
                     EELLAHIAENNKPSVKRFLKELIGAIRSWLRSKGFLSSAELSESELFALLKEVRQFAA
                     VDDGGIRYSLSNESTRPSGGFSLSGAKKAVVDAFKSEQRLAADRARENLPGEIRDSLD
                     RLGDPRTVRARVRDAARRWLTPSGLLTKDVHKLKLERDGQLNGDEFHASIRLQGFYDA
                     VKEGYQKEYEYLQPATKARLNQAMRGEDVRIPEQVKAAIAHMRDQIKRLSAIHIEQLL
                     DDAANLEEQGDDAGAAAKLRLADTISGNFETYLHRSYRAFDDPDWPNKVRENHPEVYA
                     AAVNYLANGYADGGPVTREHLDLAKKKVALMLEEGTAFDSLGAFISESKLGAKDLSII
                     KRRKTIAPEILALLGEYEDAPINYTKSVAKMSRLVYNHAFLKKLREHGFEAGLFSESA
                     NTNLGHNKKIAGDASEAYAPLNGLYTSHEFHQALVDAMGREGSGWLYDVYRHLVSINA
                     MVKFGKTVLSPPTALRNFYSAMFFSLVNGHFNFRQGRNFSWSTYFKGKPDGLAYMEKL
                     RRLGVVYDNPYAGEMMALLKDSRLDETVFNWKPFKWTKTGIDYAQKFYGFGDDLWKII
                     GFENEKAMLIKDKGMSEAAAEVEAAERIRNTYPTYSLIPKAVDALRRFPLVGTFVSFP
                     AEIIRTSYHIVRYLGNDIRELGIKNPMVQRKALGLLIAGASMYALQAITKALVGVDDD
                     EEKALRLMSPEWQKNSNLAYLGRDEKGALQTMDMSFLDPYGYFKRPINALLQDQPLGE
                     ALKSGTKDLLTPFFGQDITFGAIMDVAMNQKDTGGRVFNPEDTVANQTLDISAHIAKA
                     VQPGIVTNITRMYMASTGKVTASGKKFDVGDELAALGGFRWGTFDPKTSMYYQTFAFT
                     DAKADASKILTAAIRDPNKVDEEELRDAFDTAMRARAKAYDRMAAVVAAAEKSGLSKW
                     QINKILRDSGITNEDARALIKGDAPKWKLEQSAMRSNVRRAGVLFSQEVADEIKRRQK
                     IVREWAK"
BASE COUNT          755 a          971 c          860 g          869 t
ORIGIN      
        1 aagaaaactg gcgtgggctg ctgcttatag cgattattat tttttgcttc tgggcgggga
       61 tccagaatga accggcgccc tactccaaat agctacttgg cccactccct aacgatcttc
      121 tgtctgcgct taatttcgtc ggccacttcc tgagaaaaca gcacccccgc cctgcgaaca
      181 ttggagcgca tagctgattg ctccagcttc catttaggcg cgtcgccctt aatcagcgcg
      241 cgagcgtctt cattggtaat gccagaatcc cgcagtattt tattaatctg ccacttacta
      301 aggcctgatt tctcagcggc cgccactaca gccgccatac ggtcataggc ctttgccctg
      361 gcgcgcatgg ctgtatcaaa tgcgtcccga agctcctctt catcgacctt gttagggtcg
      421 cgaatagccg cggttaaaat cttagaggca tccgccttgg cgtcggtgaa agcaaacgtc
      481 tggtaataca tggaggtttt ggggtcaaag gttccccagc ggaaaccccc aagcgccgcc
      541 agttcatcgc caacgtcaaa ctttttgccg ctggccgtca ctttgccggt agaggccata
      601 tacatgcggg tgatgttggt aacaataccc ggctgaaccg ccttcgctat gtgcgcggaa
      661 atatctaggg tttggtttgc cacagtatct tccgggttaa acaccctgcc ccccgtgtct
      721 ttttggttca tggctacatc catgatggcg ccaaaggtaa tatcttggcc aaagaacggc
      781 gtcaacaagt ccttagtgcc agactttaaa gcctcgccca agggttggtc ttgcagcagc
      841 gcattgattg gccgcttaaa gtaaccgtaa gggtccaaaa agctcatatc catggtttgt
      901 agcgcaccct tttcatcgcg ccccaggtaa gccaggttag agtttttctg ccactcgggg
      961 gacattaggc gcagcgcttt ttcctcatca tcatccacgc ccactaaagc cttggtaatc
     1021 gcctgcagcg catacattga ggcgccagcg ataagcaagc ctaaggcctt gcgctgcacc
     1081 atggggtttt taatcccaag ctcgcgaata tcattaccca ggtaacggac tatgtggtaa
     1141 ctggtgcgga ttatttcggc cgggaagcta acgaatgtgc ccaccagggg gaaccgacgc
     1201 agcgcatcca ccgccttggg aattaggcta tacgtcgggt atgtgttgcg aatccgctca
     1261 gccgcttcaa cttcggcggc cgcctcgctc atccccttat cctttatcag catggccttt
     1321 tcgttttcaa agccgatgat tttccagagg tcatcaccaa agccataaaa cttctgcgcg
     1381 tagtcgatac cggtttttgt ccacttaaat ggcttccaat taaaaacagt ctcatcgagg
     1441 cggctgtctt tgagtagcgc catcatttca cccgcgtaag ggttatcgta aacaaccccc
     1501 aggcgccgca gcttttccat gtatgccagg ccgtccggct tgcctttgaa ataggtggac
     1561 caagagaaat ttctgccttg gcggaaattg aagtgcccat taaccaggct gaagaacatc
     1621 gcgctgtaga agttacgtag cgcagttggg gggctcaata cggttttgcc gaatttcacc
     1681 atggcgttaa tcgataccag gtgccgataa acatcataga gccatccgct gccctctcgc
     1741 cccatggcat cgactagcgc ttggtggaac tcgtgggagg tatacaggcc attcagtggc
     1801 gcataagcct cgctggcatc cccggcaatc ttcttgttgt gcccaaggtt ggtattggca
     1861 ctctcgctaa aaagcccagc ctcaaaacca tgttcgcgca gctttttcaa aaacgcatgg
     1921 ttgtaaacaa ggcggctcat cttggcgacg gacttggtgt aattaattgg ggcgtcctca
     1981 tactcgccca ggagcgccag aatctcgggt gcaatcgttt ttcggcgctt gataatgctt
     2041 aaatccttgg cgcctaattt tgactcggag ataaacgcac ccagcgaatc aaatgcggtg
     2101 ccctcctcca gcatcaaggc aactttcttt ttagccaagt ccaggtgctc gcgggtaacc
     2161 ggcccgccgt cggcataacc attggccagg taattaaccg cggccgcgta cacttccggg
     2221 tgattctcgc ggaccttgtt aggccagtcg gggtcatcaa acgcccggta gctgcggtgc
     2281 aggtaggttt caaagttgcc cgaaatggtg tctgccaggc gcagttttgc cgcggcccca
     2341 gcatcatcgc cttgttcttc caggttggcg gcatcatcaa gcaattgctc aatgtggata
     2401 gcagacagcc gcttaatttg gtcgcgcata tgggcaatgg cggctttaac ctgctcgggt
     2461 atgcgcacat cttcaccgcg catggcttgg tttaatctcg ccttggtggc cggctgcagg
     2521 tattcatatt ccttttggta gccttctttt accgcatcat aaaaaccctg caggcgaata
     2581 gatgcgtgga attcatcgcc atttagctgg ccgtctcgct ccagttttag cttgtgcaca
     2641 tcctttgtca gcaatccgct gggggtcagc cagcggcggg cagcatcgcg aacgcgagcg
     2701 cggaccgttc tggggtcacc caatctatcc agggaatcgc gaatttcccc tggcaagttt
     2761 tcgcgcgctc gatcggcagc aaggcgctgc tcagacttaa aggcatccac caccgctttc
     2821 ttggccccag ataaagaaaa cccgccactg gggcgggttg attcgttgct taggctgtat
     2881 ctaattcccc cgtcgtcaac ggccgcaaac tgccgcacct ccttaagcag cgcaaacaat
     2941 tcactttcgg ataattccgc agaggataaa aaccccttag atcgcaacca ggagcgaatc
     3001 gcaccaatta attctttaag gaatcgctta acgctgggtt tattattttc tgcaatgtgg
     3061 gccaacaatt cctcagtcat aaaaccaagg cgctcagccg gttttgcgct ggcataagct
     3121 gcttggtatt tttccaaggg catagcgtat ttggtggcaa gctcgcgcag cgtcttgtct
     3181 ccacccatcg ccaagtacaa gctacccata gccgcctgaa tatcctggcc aaacattttg
     3241 gcgaggccga cgtgggctaa ttcatgtaaa ataacagtct caacatcctg tgaagtttgc
     3301 agggcctcgc gatttacaaa gatgctgccc ccgtggaata cgccttttac cttgttgcct
     3361 gcaatcccgt ttttctccat gtgcgcttgc acttctggcg ggagctcaac cgcgctcgca
     3421 actgcagtta ttttgagttt cgcgccaaag ttgga
//
LOCUS       DAOQGS010000448         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15674_length_3454_cov_5.894675, whole genome shotgun sequence.
ACCESSION   DAOQGS010000448 DAOQGS010000000
VERSION     DAOQGS010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15674_length_3454_cov_5.894675"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="PKZ52_14450"
     CDS             <1..1658
                     /locus_tag="PKZ52_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61021.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSGFAFSGSFTPLTNQGPDERVTSE
                     TITLTGVNATKTLKLTSDAKAGYSIDGGDFISTDSEIPAGVHTLAVRLVTSTAASTPA
                     SASLSIGGQAVVFTATTRADITPPTGTISFPPKISLTEGTTVIMRGTVKDEASSIKSV
                     KINNQTATFDADKGTWELKAATLSPGTQTLLLEVEDSAGNKNTRAASIDITQGDITKT
                     FPANGGVDFVGPQTVAWDNLDGRNRALVMDVDAKALIAVDLNTGVRSVLSDNTTQNDL
                     PFIYEGKGYNAGSIVLDKKVGKVYAGLSIDGEGSNLDLPHSIIGVDVLTGTRSIVSNS
                     VFFETSDMVLDTTGSNYRLFAGETKEDYLSGWDFISNSGFDYSSYSLGQPDKVNQFYN
                     VGSLAVDAPRKRLLMTSLSGQQFVYAVDIAAASNAPFQNKGARSVFSDGATPNVENPF
                     TSSGNYVLTSIRVDSDRSRALMVDRLKPAIFELALSDDKTKDGARSVLSDNATANKLA
                     NPYGLHIESGMPYALLVDKGTKALIAVDSESGERVFISKTSAY"
     gene            1697..2872
                     /locus_tag="PKZ52_14455"
     CDS             1697..2872
                     /locus_tag="PKZ52_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61022.1"
                     /translation="MSDDFTKLTMGAAGVNMQCHPHELMKLNSAPFVEVLRGASAPYG
                     YQIFLTGYSDMVALKEQELSADSARQLSQDFHLIKSNPRMLSNHKLNGSVDTWSAQGR
                     YYRMDYKVSNGQVVIFNIQPLDHIQKSRDLLEKPSLYSVAKNSEGGWHIKSKVDVAKE
                     GYGAVNGQSNNLTKATWLMGAHLEFEYGNQVKEFTLFHNPSVGGPGDTWESFRDKLGI
                     TTPVTKVFAEKLLAAQQANQTIKWVAHSQGGVIFAEAVRTILKKNKKQTRILDKQSIA
                     LHGNANNNFRSSFLFDKAGIEVIASRGNTYDLVYVFAGLNTLNPWRMIGAAVYWRHVF
                     SGSVNQSTHTMQHKDYDAWNQEMTFGAGRGRSPIQKGFHAVDQLGRKAIKAIPNYLK"
     gene            2878..>3454
                     /locus_tag="PKZ52_14460"
     CDS             2878..>3454
                     /locus_tag="PKZ52_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61023.1"
                     /translation="MKKSIFGACFFMTFLTSLIAGAKEPVKFERVVRLPDAELRFSMT
                     EDFSKDMPAAPLVEKVTIDRLQDLKAPQDRFVIGRRWWDLKPPGFFKKNWGSVQMTVA
                     IGASIDLHGEKLDFCKSSQTDFLIFYRGSLLDKWRAHNATVAPEDKLKYSVDISPFFD
                     SVGQRFVSYFEYKKNSHQVVVLEGVGGRDADI"
BASE COUNT          944 a          712 c          876 g          922 t
ORIGIN      
        1 caagctccag ttcatccagt tcaagctcta gctcgtcaag ttctagctcc agctccagct
       61 ccagcggctt tgcctttagc ggcagcttta cccctcttac taatcaaggc ccggatgagc
      121 gcgttacctc cgagaccatt actcttaccg gtgtaaatgc caccaaaacc ttaaagctca
      181 ccagcgatgc caaagcgggc tactcgatag acggcggcga cttcattagc accgacagcg
      241 aaatccctgc cggagtacac accctggccg ttcgcttagt taccagcacc gccgccagta
      301 cccctgcatc agctagcctc agcatcggcg gccaggccgt agtctttacc gccaccaccc
      361 gcgccgatat aaccccgccc accggcacca ttagcttccc gcccaaaatc agcctcaccg
      421 aaggcactac cgtcatcatg cgcggcaccg ttaaagacga agccagcagc attaaaagcg
      481 taaaaataaa taaccaaacc gccaccttcg acgccgacaa aggcacctgg gaattaaaag
      541 ccgccacgct aagcccaggc acacaaacct tattgttaga agtggaagac agtgcaggga
      601 ataaaaacac ccgtgccgcc agcatcgata ttactcaggg cgatataacg aaaacattcc
      661 ccgcaaacgg cggtgtggat tttgtggggc cgcaaaccgt ggcttgggat aatttggatg
      721 ggcgcaaccg cgccttggtc atggatgtgg atgccaaagc attaattgcg gtggatttaa
      781 ataccggggt gcgcagtgtg ttgagtgaca acacgacgca aaatgatttg ccgtttattt
      841 acgaagggaa aggctataac gcaggttcga ttgttttaga taaaaaggtt ggaaaagttt
      901 atgcgggatt gtcgattgat ggtgaaggct cgaacctaga tctgccacat tcaatcattg
      961 gtgttgatgt actaaccggt acgcgctcca ttgtgtccaa ctcagttttt tttgaaacct
     1021 ctgatatggt gttagatacc accggctcaa attatcggtt attcgcggga gagactaagg
     1081 aagactattt aagtggctgg gactttatta gtaattcggg gtttgattac agttcctatt
     1141 cattggggca gcctgacaag gtaaatcaat tttataacgt gggcagttta gctgtggatg
     1201 ccccgcgcaa gcgcctgctt atgaccagtt taagcggcca gcaatttgta tatgccgttg
     1261 atattgcagc ggcgagtaat gcgccatttc aaaataaagg tgccaggtcg gtgttttctg
     1321 atggcgccac tccaaatgtg gaaaacccat ttacctcaag cggcaattat gttttgactt
     1381 ccattcgagt ggattctgat cgtagtcgcg cactaatggt ggatcgtcta aagcctgcaa
     1441 tttttgaatt agcgttaagc gatgataaga ccaaagatgg tgcgcgaagt gttttgtccg
     1501 ataatgctac agccaataaa ctcgctaacc cttatgggtt acatattgag tcaggtatgc
     1561 cctatgcgct tctggtggat aaaggcacaa aagcactaat tgcagtggat tcagagagtg
     1621 gtgagcgagt ttttatatct aaaacctctg cctattaagt tgacggtagt tatcaggatt
     1681 aatttaggag ttatctatga gtgacgattt tacaaaatta actatgggag ctgctggtgt
     1741 gaatatgcag tgccaccctc acgagcttat gaagttaaat tcggccccat ttgttgaggt
     1801 gttgaggggg gcgtctgctc cttatgggta tcaaatattt ctcacagggt acagcgatat
     1861 ggtggcacta aaagagcaag aattaagcgc tgatagcgct aggcaattgt ctcaggactt
     1921 tcatttaata aaatccaatc ctcgtatgct gtctaatcac aaactgaatg gtagtgtaga
     1981 tacatggagt gcccaaggac gctattatcg aatggattat aaagtctcta atggtcaggt
     2041 tgttattttt aatatccaac ccctggatca catacagaaa tcaagagatt tgttagaaaa
     2101 acctagtctt tatagtgtgg ctaaaaatag tgagggtggt tggcatatta aaagcaaagt
     2161 ggatgtggcg aaggaaggct acggtgcggt gaatggccag tcaaataatt taaccaaggc
     2221 tacatggttg atgggggcgc atttggaatt tgaatatggg aatcaggtga aagagttcac
     2281 cttgtttcac aatccaagtg tcggtggtcc gggcgatact tgggaatcat ttcgcgataa
     2341 gttaggcatt accaccccgg tgaccaaagt ttttgcggaa aaattgctgg ctgcacagca
     2401 ggctaatcag accattaaat gggttgcaca cagccaagga ggggtaatat ttgcggaggc
     2461 agttcgcacc attcttaaaa agaataaaaa acaaacgcgc atcttggata agcagtcaat
     2521 agctttgcac ggcaatgcta ataataattt ccgctcaagt ttcttatttg ataaggcggg
     2581 tattgaagtg attgccagcc gtgggaatac gtatgacttg gtgtatgtgt ttgcagggct
     2641 taacacactt aatccgtggc ggatgatagg tgctgcagtt tattggcgtc acgtattttc
     2701 tggctcggtt aatcaaagca ctcatacaat gcagcataaa gactatgacg cgtggaacca
     2761 ggaaatgact tttggggcag ggcgaggccg cagccctatt cagaaaggct ttcatgcagt
     2821 cgatcagttg gggcgtaaag cgattaaggc aatacctaac tatttgaaat aaatacaatg
     2881 aaaaaatcta tttttggggc ttgttttttt atgacatttc taacatcact aatagctgga
     2941 gctaaagaac ctgtaaagtt cgagcgagtg gttcggctgc cggacgctga gttgcgtttt
     3001 agtatgaccg aagatttttc taaggatatg cctgcggcgc ccttggtgga gaaagttacg
     3061 atagataggt tgcaggattt gaaggcgcct caggaccgtt ttgttattgg gcgacgttgg
     3121 tgggatttaa agcccccagg ttttttcaaa aaaaactggg ggagtgtgca aatgaccgtg
     3181 gcgattggtg cgagcataga cttgcatggg gaaaaattag atttttgtaa gtctagtcag
     3241 acagattttt tgatttttta tcgggggtca ttgcttgata agtggcgagc acataatgct
     3301 actgtggctc ctgaggacaa gttaaaatac tcggtagata tttcgccatt ctttgactct
     3361 gtagggcaaa gatttgttag ttattttgag tataaaaaaa attctcatca ggtggtggtg
     3421 cttgaggggg ttggagggcg tgatgcggat atat
//
LOCUS       DAOQGS010000449         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15697_length_3452_cov_4.168973, whole genome shotgun sequence.
ACCESSION   DAOQGS010000449 DAOQGS010000000
VERSION     DAOQGS010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15697_length_3452_cov_4.168973"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKZ52_14465"
     CDS             complement(<1..182)
                     /locus_tag="PKZ52_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61024.1"
                     /translation="MNYYWSASVGVVLLAALIYLGGRYLPAPGAQEQTIKPTNAMVQE
                     ANTQQGSAAENIPTGQA"
     gene            578..1297
                     /locus_tag="PKZ52_14470"
     CDS             578..1297
                     /locus_tag="PKZ52_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG61025.1"
                     /translation="MQSPLDVSPAILLKAVAFGWAAAQSLLHIPHLCIEQGQHVFLYG
                     PSGSGKSTLLNLIAGISLANTGSVEILGTNLSALPQRKRDEFRAQHLGIIFQQFNLIP
                     YLNLLDNLLLRAKFAQAANPQEDKNTRRQAQDLLARLGLGAQLQQKAQDLSVGQQQRV
                     AVARALLCEPKIIIADEPTSALDADNKNSFIDLLLSSAQQFNSTIIFVSHDKSLEGYF
                     NRAIDIQAFHPAQHRSSAHDI"
     gene            1287..2534
                     /locus_tag="PKZ52_14475"
     CDS             1287..2534
                     /locus_tag="PKZ52_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61026.1"
                     /translation="MIFKLARLSLWHRRSTVLLTLLSLSFSIALVLGVDHLRNQAKIS
                     FNKTLSGTDLIVGARSGSINLLLYAVFRIGNATNNISWQSYQELAKHSAVDWVIPLSL
                     GDSHKGYRVLGTTQDYFTHYQYGQQQALSLAKGKAFSALYEVVLGAEVAAKLGYTIGD
                     TIIIAHGSGETNLSKHDKQPFVVSGILAPTGTPVDRTLHTSLGSIEAIHLGWRAGVPN
                     ESAPQAAQQADLTPSSITAMLVGLKSRTAIFNVQRQINEFPQEPLLAIVPGVALTELW
                     QMLAMVEHLLMVIAVSILLAALLGMVTTLLAAMNERQREIAILRSLGAKASFIFILVE
                     LEIMLILGGAFVLAGGALCLSLAAGQPYLAQYFGLFISSNPLQSYTPMAASLVVLLAL
                     LLGAAPASAAYRRALSDGLRQRY"
     gene            complement(2548..>3452)
                     /locus_tag="PKZ52_14480"
     CDS             complement(2548..>3452)
                     /locus_tag="PKZ52_14480"
                     /inference="COORDINATES: protein motif:HMM:NF021624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HNG61027.1"
                     /translation="FHDDFSPENTAFYFHEFAAAAAGAGLQYLTEAEFSDTQSLQFSP
                     EVQATLSQLGQDSILAQEQYLDFLTGRNFRQTLLCHHEKPLTRPLAAAHMRNFYFSIN
                     VQPEQPVDSVNNAALMRFAHPELGAMSTDNPLAKAALSILGAQFPKALKFADLVAQAQ
                     AMLADNSPVDEPRRAELAEFLCAALRRGIAQIHTEPHNLPEQAGEFPVASAIARQQAQ
                     HSPVITTLLHDTVSLDDPLSRAMLVLLDGTRNRAALVEELKTRAKAPQETQAQAEYAQ
                     FIANLPELVETQLKRLIRLGLLLQ"
BASE COUNT          858 a          866 c          853 g          875 t
ORIGIN      
        1 gcttgcccag ttggaatatt ctctgcggcg ctaccctgtt gtgtgttggc ctcctggacc
       61 atggcattag ttggcttgat tgtttgctct tgcgccccag gcgctggcaa ataacggcca
      121 cccaaataaa ttaatgccgc cagcagaaca acacccacac ttgcagacca ataataattc
      181 atttcacctt acctaattgt gtgttttact gccgctctga taaattaccc ggcagcctga
      241 atgctacgga taacgcagat tagagcatat tttaacagcc ggttgaaata ccaccgtgcc
      301 agtaatggcg gcgcttaggt atttctcccg gccattacga gcgcgactta cgtaataaat
      361 cggcatgagt tgagagcgtg aaaaataagt acttgcctta cgcttctact taagcggttt
      421 taaagttaaa cggtttaaag ttaaagagta ttaaagttaa gaagtattaa aggccagtct
      481 gaaatcatgc gtatttatta cagatatttt atagcagtgc gcggcgcaaa gttatcccac
      541 acgccgtttt tcaatcattt ttattgaacc caattgtatg cagtcaccat tagatgtttc
      601 gcctgctata ttactcaagg cagttgcctt cgggtgggct gccgcccaat cgctgctgca
      661 cataccccac ttgtgtattg aacagggcca acacgtcttt ttatatggcc cctcaggctc
      721 tggtaaaagc accctgctaa acctaattgc aggcattagc cttgcaaaca cgggcagcgt
      781 ggaaatactg ggcaccaatc ttagtgccct gccccagcgc aagcgcgatg aattccgcgc
      841 gcagcatttg ggaattatat tccagcagtt taaccttatc ccttacttaa acctattgga
      901 taacctactc ttgcgggcga agtttgcgca ggccgccaac ccacaggagg ataaaaacac
      961 tcgccgccaa gcgcaggatt tattggcccg tttaggccta ggcgcacagc tgcaacaaaa
     1021 agcgcaggat ttaagcgtcg gccagcagca gcgtgtggcg gtggcccgcg cgcttttatg
     1081 cgagccaaaa ataattattg ccgacgaacc cacctcggca ttagatgcag acaacaaaaa
     1141 tagttttata gatttattgc tcagcagtgc ccagcaattt aacagcacga ttatttttgt
     1201 cagccacgat aaatcattag aaggttattt taaccgggcc attgatattc aagcctttca
     1261 tcctgcccaa cacagaagct ctgcccatga tatttaaact ggcgcgctta agcctgtggc
     1321 accgccgcag cacggtactt ttaaccctgt tgtcgttaag ttttagcatt gcactggtgc
     1381 tgggggttga ccatctgcgc aaccaagcca aaatcagctt taataaaacc ctctccggca
     1441 cggatttaat tgtcggcgcc cgctccggta gcattaattt attgttgtac gcggtatttc
     1501 gcatcggcaa cgccactaat aatattagct ggcagagcta ccaagaatta gccaaacact
     1561 ccgccgtgga ctgggtaatt ccgctgtcgc tgggggattc ccacaagggt tatagagtgc
     1621 tgggcactac tcaagattat tttacccact atcaatacgg ccagcagcag gcgttaagcc
     1681 tcgccaaagg caaagcgttt agcgctttat atgaagtagt tttaggcgct gaagtggctg
     1741 caaaactcgg ttatacaatt ggcgacacaa taattatcgc ccacggcagt ggtgagacaa
     1801 acctcagcaa acacgataaa caaccgtttg tggtgagcgg aattttagca cccactggca
     1861 caccggtgga tcgcacactc cacaccagcc ttggctctat agaggccatt cacctgggtt
     1921 ggcgagccgg tgtgccaaac gaaagtgctc cacaggctgc ccagcaggcc gatttaacac
     1981 ccagcagtat taccgccatg ctcgtggggc ttaaatcccg cactgccata tttaatgtgc
     2041 aacggcaaat taatgaattc ccgcaggagc cactgctggc gatagtacca ggcgttgccc
     2101 tcaccgagct gtggcaaatg cttgccatgg tggagcattt attaatggtg attgcggtat
     2161 ctattttatt ggcggcttta ctgggcatgg taaccacact attagccgct atgaatgagc
     2221 gccagcggga gatagcaatt ttgcgctccc tcggcgccaa ggcgagtttt atttttatac
     2281 tggttgaatt ggaaattatg ttaatccttg gcggcgcatt tgtgctggca ggcggcgcgc
     2341 tatgcttaag ccttgccgca ggccagccgt atttagcaca gtattttggt ttatttatta
     2401 gcagcaatcc cctgcaatcc tatacgccga tggcggcaag cttagtagtg ttattagcgc
     2461 tattattggg tgcggcgcca gcgagcgccg cgtataggcg cgccctgagc gatggcttgc
     2521 ggcagcggta ttgaaataaa cctgcaatta ttgcagcaat aaacccaagc gaattaagcg
     2581 ttttaattgt gtttccacca actccggcaa attggcaata aattgcgcat attctgcttg
     2641 ggcctgagtt tcttgcggag ctttagcgcg ggtttttaac tcctccacca gggcggcgcg
     2701 gttacgcgtg ccatctaata acaccagcat agcgcggctg agtgggtcgt ccaggctgac
     2761 cgtatcgtgc aataaggttg taattaccgg gctgtgctgg gcttgctggc gggcaatagc
     2821 actggcaaca ggaaattcac cggcctgctc cggcaggttg tgtggctcgg tgtgaatctg
     2881 ggcgatacca cggcgaaggg ctgcacataa aaattccgct aattccgccc ggcgcggttc
     2941 atccaccgga gaattatcag caagcattgc ctgggcctgc gccaccaggt ctgcaaactt
     3001 tagcgctttg ggaaactggg cacctaaaat actcaatgcg gccttagcca gagggttatc
     3061 ggtgctcatg gcgcctaatt cagggtgcgc aaaacgcatt agagcggcat tattcacgct
     3121 gtctactggc tgctccggtt gcacattaat cgagaaataa aaattgcgca tgtgtgccgc
     3181 tgccagcggg cgcgtgagag gtttttcgtg atggcacagc agcgtttggc gaaaatttcg
     3241 gccggttaaa aaatctaaat attgctcctg ggctaatata gaatcttgcc ccagctggct
     3301 gagcgtcgcc tgcacctcag gggaaaattg caggctttgt gtatcgctaa attctgcctc
     3361 cgtcaggtat tgcaaacctg ctccggcagc ggcggcagca aattcatgga aataaaacgc
     3421 ggtattttca ggtgaaaaat catcgtggaa aa
//
LOCUS       DAOQGS010000450         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15727_length_3449_cov_4.642899, whole genome shotgun sequence.
ACCESSION   DAOQGS010000450 DAOQGS010000000
VERSION     DAOQGS010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15727_length_3449_cov_4.642899"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKZ52_14485"
     CDS             <1..636
                     /locus_tag="PKZ52_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61028.1"
                     /translation="EIREVIHEFKQGFQLGHATSGATGYRIAWGGQFENQQRAASRLS
                     LVVPVSLFLIFVLLFSTFRSLRQALLILSIIPFALVGGIVALWLTGEYVSVPASVGFI
                     ALLGITVLNGVVLVSYFNQLYAQGLDMFEIVSQGARRRLRPVMMTASITAFGLIPLLF
                     ATGPGSEIQRPLAIVVIGGLVTATALTLILLPILYQRFAFAKPANRGISHV"
     gene            629..931
                     /locus_tag="PKZ52_14490"
     CDS             629..931
                     /locus_tag="PKZ52_14490"
                     /inference="COORDINATES: protein motif:HMM:NF023014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HNG61029.1"
                     /translation="MSDICLTLLCPIAHQEHLIDHLLLASDVQVFASSNGAAHGLHPR
                     ELSASEQVLGQARAVQITALVSKADSETVLAALKHEFAGIGLRYWLLPVIEAGEIL"
     gene            928..2175
                     /locus_tag="PKZ52_14495"
     CDS             928..2175
                     /locus_tag="PKZ52_14495"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNG61030.1"
                     /translation="MKYWLFGLLAALSSGIASAAEPQIETPGLLPTSVARTILDQDPE
                     VTAARAGSEVFKEEAALAKGSPYEWTVKYTAQKRKLEAGDEEYNEWNAALERTVRLPN
                     KIRADKRIGNALLDRGEAEYGEALHETAKELLNLWLDWLAAEQAYTLTQANQQSTQDN
                     LGIVQKQFKAGDAAKLEVALAQGELAEQQRASSTAKIKASVAWSKVHARFPGLTREYA
                     NLPEARPVNLPESYWRERISSQSDELKIAEAHYREAQANGERARADRVPDPTLGVFSA
                     SEFAGRERVVGLSISLPLSGSQRSAKARKAVQTAYVAHDDWILQQRENEARIAAAVAT
                     ASGHYESWQLAEASAAALSENVRLMQRAYTLGEADLSTLLLARRQATAASLAALEAKV
                     ESARAYYSLLIDAHLVWDLDHEK"
     gene            complement(2758..3249)
                     /locus_tag="PKZ52_14500"
     CDS             complement(2758..3249)
                     /locus_tag="PKZ52_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61031.1"
                     /translation="MQYHLLKIGNALGYDVISATNDRSRCHAGNNFSFISLDNFPIID
                     IDKDTLNTIALIDVVWFEKGTNRIIGAFEVEKSTSIYSGILRLTDLHYSFPENNASLY
                     LVIPDNREKELISQLMRPAIKQSNVQIHYLVFSDLSKYCDAICTFGENVDALKKIARI
                     ACL"
BASE COUNT          917 a          840 c          851 g          841 t
ORIGIN      
        1 gaaatccgcg aggtgatcca cgagttcaag cagggtttcc agcttgggca cgcgacctcg
       61 ggagccaccg gctatcgcat cgcctggggt ggacagtttg agaatcagca gcgcgcggct
      121 agccgcttaa gccttgtggt tccagtgtcg ttatttctga tttttgtgtt gctgttttcc
      181 acgtttcgct ccctgcggca ggcactgctg atcctcagca ttattccttt tgcactggtc
      241 ggcggcatag tcgcgctctg gctgacgggt gaatatgtct cggtgccagc atccgtgggt
      301 tttatcgccc tgctgggcat cacggtactc aatggtgtgg tactggtgag ttacttcaac
      361 cagctttatg cccaagggct ggatatgttt gagattgtca gccaaggtgc gcgccgacgc
      421 ttacgcccgg taatgatgac ggcgtccatc acggcatttg ggttgatacc tctgttattt
      481 gccaccggcc ccggctccga aatccagcgc cccctcgcga tagtggttat cggcggcttg
      541 gttacagcca ctgcactcac acttattttg ctacccattc tttaccagcg gtttgccttt
      601 gcgaaacccg ccaaccgggg gatttcccat gtctgatatc tgcttaacgc tgttgtgtcc
      661 catcgcccat caagaacatt taatcgacca tttgctgctg gcgtctgacg ttcaggtatt
      721 tgccagcagc aatggtgcgg cgcacggctt acacccacgc gaactgagtg ccagtgaaca
      781 agtgcttggc caagccagag ctgtgcagat tacggcattg gtaagcaagg ccgatagcga
      841 aacggtactt gcagccctta aacacgaatt tgcaggtatc ggtctgcgct attggctgtt
      901 acctgttata gaggcaggag aaatcctatg aaatattggt tattcggcct attggctgcg
      961 ttaagttcgg gtatagcgag tgcggcggaa ccccagattg aaaccccggg cttgttgcct
     1021 acatcggtgg cgcggaccat tcttgatcaa gatccagaag tgacagctgc ccgtgcaggt
     1081 tcagaagtat ttaaagaaga agccgccctt gctaaaggct cgccctacga gtggacggta
     1141 aaatacacgg cccaaaagcg caaactggaa gcgggtgatg aagaatataa cgagtggaac
     1201 gccgccttag aacgcaccgt acgcctgcca aacaaaatcc gcgctgataa gcgtatcggc
     1261 aacgctctat tagatagagg agaagcagaa tacggtgagg ccttgcatga gacggctaaa
     1321 gagttactga atctgtggct ggactggctg gccgccgaac aggcctacac gctaacccag
     1381 gctaaccaac aatccacgca ggacaatctc ggcattgttc aaaagcaatt taaagcgggc
     1441 gacgccgcca agctggaagt ggccttggca caaggtgaac tggctgagca gcaacgcgcc
     1501 agcagtacgg ccaaaattaa ggcctctgtt gcctggtcaa aagtccatgc ccgttttcca
     1561 ggactgaccc gcgaatacgc caatctacca gaggcccggc ctgtcaattt gccggaaagc
     1621 tattggcgcg aacggatttc cagccagagt gacgaactca aaattgccga ggcgcattac
     1681 cgtgaggcgc aagctaatgg cgagagggct cgcgctgatc gagtacccga tcctaccctg
     1741 ggcgttttca gcgcatcaga gtttgctggc agagaacggg ttgtaggcct atcgattagc
     1801 ctacccctct caggctccca gcgcagcgca aaggcccgca aagcagtaca aactgcctat
     1861 gtagcgcacg atgattggat actgcagcag cgtgagaatg aagcgcgaat agcagcagct
     1921 gttgctaccg ccagtggaca ttacgaaagc tggcaattag cagaagcaag tgctgcggcc
     1981 ctgtcagaaa atgtccgcct tatgcagcgc gcttatacct tgggcgaggc tgatttatcc
     2041 acgcttctgc ttgccaggcg ccaggctaca gccgcatcac tcgctgccct ggaggccaaa
     2101 gttgaatcgg cacgcgctta ttacagctta ttaatcgatg cgcatttagt ctgggattta
     2161 gaccacgaga aatagccgca ttagagcgca aatgagcaag caagaatata cggaaagcaa
     2221 taaaaatcat ttaaattaat acaccatcaa tctaactgag tatccaccat gaaaaaattc
     2281 gcatcccatt atattaatgg gtttgttaat aggcacccct gttctggccc atgaatctga
     2341 ttttcatgtt agcgatcaga gcgcaccagc cgtacgaaaa agtgcagcag ctgaatttag
     2401 caaaaatgac agtgagctaa gcttcggcaa gttacctccg agctggatgg acatgtgttt
     2461 aaaaaatgcg accattttaa aaaaatgaca gaggtatata tgtcctactc tcatattcag
     2521 tttattaact tgcgggagat agtttgcttg atggagacat tcattcgagt gaatagactt
     2581 aattctcttt atgaacaatc ttgagaggca aatgaccatc gactttaaat ggtagggcaa
     2641 catccttaat ttaggcaatt aaatcaccta gcagaggcct ctagatttga cggaaagtaa
     2701 ggtgaacaat aaaaggatta aatgttattt cgaaaaatct gcacgatccg ctagtattca
     2761 taaacaagca attctagcga tcttttttaa tgcgtctaca ttttcaccaa aggtacatat
     2821 cgcgtcgcag tacttgctta gatctgaaaa taccaggtaa tgtatttgga cgttactttg
     2881 tttgatggcc ggtcgcatta actgtgaaat aagttctttt tccctgttat ccgggataac
     2941 tagatacaag ctcgcgttgt tttcagggaa cgaataatga agatccgtca gccgcaaaat
     3001 acccgagtaa atggacgtac ttttttcaac ttcgaaggcc cctataattc gattcgttcc
     3061 tttttcaaac cagacaacat ctatgagggc aatagtattg agcgtatctt tatcaatgtc
     3121 gattatagga aaattgtcca aactaataaa cgaaaaatta tttccggcgt ggcagcgcga
     3181 acgatcattg gtcgcactaa ttacatcgta tcctagagcg ttgccaatct tcagtaaatg
     3241 gtattgcatt tcagtatgca ggttttcttc ctcgtgctct gatccctacc actaaaagtg
     3301 gacaccctaa aaacggttag aataacccga gaggtgaccc atgaccaaga agactcccgc
     3361 acaatcgaac aaaaaacaca ctgctgaatt caagcttgag gctctaaaac tggccgatag
     3421 agtgggtgcg acagaagcag ctgaaaaac
//
LOCUS       DAOQGS010000451         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15750_length_3446_cov_4.599233, whole genome shotgun sequence.
ACCESSION   DAOQGS010000451 DAOQGS010000000
VERSION     DAOQGS010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15750_length_3446_cov_4.599233"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..735)
                     /gene="greB"
                     /locus_tag="PKZ52_14505"
     CDS             complement(226..735)
                     /gene="greB"
                     /locus_tag="PKZ52_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNG61032.1"
                     /translation="MSRYRPPAPKSAPYITREGWQTLQAELQFLWHEERPKVTQVVHE
                     AAKNGDRSENGDYIYGKRRLREIDRRIGYLSRRLDVLKIVDSTPDDLSKIYFGAYVTL
                     EKDDGSTITYRIVGPDEFDLKAHKISLDAPLAKALLGKTLDDEIRLTLADKVDIYYVV
                     DVRYGPLSD"
     gene            complement(732..2615)
                     /locus_tag="PKZ52_14510"
     CDS             complement(732..2615)
                     /locus_tag="PKZ52_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNG61033.1"
                     /translation="MPNHQSPPKQAPYGFWESPISVDAVLANAIQPMYPQGASGWLFW
                     LEARPAQKGRQVIVAQLPDGTKRELLPEEHSARSQFLEYGGCPFWLAGKELIWGNAQD
                     QNLYAMDFARPAAPRKLTQSGGRYADIQWDTRRNRILAVMETPSDGLSSLSIASIDLS
                     LEEQTPRVLQQGADFYAYLSLAPNGQQLAFIQWHQGHMPWDATALMLAEVNPDGSLAE
                     PATWVAEEAQQAIAQPQFGPDGCLYWVSDATNWWNLYRRQGEKVEALAPMEAECTTPR
                     WVSGMSSYCCVDADEILFCYTQGGLWNLARLNTQNGQWQPLLTDYPHISQLSSADGGA
                     LFLAAEPQGPIQIYHYSDGQVQPLAAPAPDELQAYYSQPKSIYVPSDGYNLHAFYYPP
                     HNPDYSEPEGLPPVIVLCHGGPTGQTSALLNLKNQYWTSRGFAVLDVNYRGSTGFGRA
                     FRHSLHGAWGQADVADLCNAAQWVSAQGLAGLRFIKGSSAGGFSVLAALAFHHSFDGG
                     VSLYGIGDLALLAQDTHTFEARYLDSLVGPYPEQAQVYAERSPLFAADQIRCPVLIFQ
                     GLLDKVVPPNQAQDLVATLKHQGTAVTYVEFAEEGHGFRQAQSIREQLAAEQVFYQQV
                     ISQ"
     gene            2666..>3446
                     /gene="tilS"
                     /locus_tag="PKZ52_14515"
     CDS             2666..>3446
                     /gene="tilS"
                     /locus_tag="PKZ52_14515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNG61034.1"
                     /translation="MAAYGLAQGNGAQGYCVALSGGLDSMVLAHLAKTYLPQVRLLHI
                     NHGLSPFADQWQQQVEHWAAEQNLPCHSQRVLVARQGESLEAAARKARYAVFEATLSP
                     GEALLCGHHLNDQAETFFLRLLRGSGLAGLTGMAPARPIGAGVLLRPLLGLERGALLA
                     YAQAQGLTWVEDESNSDERFERNWLRHSLLPQLTSRWPKAAAQVANTCLRLQEEQQLL
                     QEYLASDLASCSPRPERLGHSLDLTCLQQGSSARRNSLLRLW"
BASE COUNT          673 a         1000 c         1019 g          754 t
ORIGIN      
        1 ccatattcag cgtcttaggc accctcgcta cgagttcaac tgcggttttt aggttcaagc
       61 cggtgtattt tgctgtgtgc cagctgctat agcgccctcc cctttttaag gggaggggtt
      121 tgtggggcaa gcaaaaatcc agatgctaaa gacggcacaa aacacttaac ctaatggcat
      181 tgcgcaccat ccccccgcaa aactcccctt tgatagcccg aaaatttaat cactaagcgg
      241 cccgtaccgg acatccacca cataataaat atccaccttg tccgctaagg ttagcctaat
      301 ttcatcatct agggttttac ccaagagggc tttagccagc ggcgcgtcta ggctgatttt
      361 atgggctttt aaatcgaatt catccggccc cactatgcgg taggtaatcg tgctgccatc
      421 gtctttttcg agcgtgacat aggcgccgaa ataaattttg cttaggtcgt ccggtgtgct
      481 gtcaacaatt tttaatacat ctaaccggcg gcttaaataa cctatgcgcc ggtcgatttc
      541 ccttaggcgg cgtttgccgt aaatgtagtc gccattttcc gagcggtcgc catttttggc
      601 cgcctcgtgt accacttggg taaccttcgg ccgctcctcg tgccataaaa attgcaattc
      661 agcctgcaga gtttgccagc cttcgcgggt gatatagggc gcgcttttgg gggcgggggg
      721 gcggtagcgg ctcattggct gatcacctgc tggtaaaaca cctgctccgc tgccaattgc
      781 tcgcggatgg attgagcctg gcgaaagccg tggccctcct cggcgaactc cacataagtt
      841 accgcggtgc cctggtgttt gagggtggcc actaagtctt gggcctggtt ggggggcact
      901 actttgtcta acagcccttg gaatatcagc accgggcagc ggatttgatc cgccgcaaac
      961 aagggcgagc gctcggcgta cacctgtgct tgctcagggt aggggcccac tagggagtcc
     1021 aaataacgcg cctcaaaggt gtgggtgtct tgcgcgagca gtgccaaatc cccaatgcca
     1081 tacaggctta cgccgccgtc aaaactgtgg tgaaaggcca gggccgccag cacgctaaag
     1141 ccaccggcgc tgctgccttt gataaagcgc aggcccgcca agccctgggc gctcacccat
     1201 tgggcggcgt tgcacaaatc cgctacatct gcctggcccc aggcgccgtg caagctgtgg
     1261 cgaaaggcgc ggccaaagcc tgtgctgccg cggtaattca catccagcac cgcaaagcca
     1321 cggctggtcc agtattggtt ttttaaattc agcagggctg aagtctgccc ggtggggccg
     1381 ccgtggcaga gcacaatcac cggcggcaag ccctcaggtt ctgagtaatc cgggttgtgg
     1441 ggcgggtaat aaaaggcgtg caggttgtag ccgtcgctgg gcacataaat gcttttgggt
     1501 tggctgtaat aggcctgcag ctcatcaggg gcgggcgctg ccaggggttg tacctgtccg
     1561 tcgctatagt gataaatctg tattgggcct tgaggctcgg cggctaaaaa cagcgcgccg
     1621 ccatctgcgc tactcaactg gctgatatgg gggtaatcgg tcagcagtgg ctgccattgg
     1681 ccgttttggg tgttgagcct ggccaggttc cacagccccc cttgggtgta gcagaagagg
     1741 atttcatccg cgtccacaca acaataactg ctcatgccgc ttacccagcg gggcgtggtg
     1801 cattcagcct ccatgggggc cagggcctca accttctcgc cctggcggcg gtagaggttc
     1861 caccagttgg tggcatcgct cacccaatac aggcagccgt cggggccaaa ttgaggttgg
     1921 gctatggcct gttgcgcctc ctcggccacc caggtggcag gctcggcgag gctgccgtcg
     1981 gggttgacct cggccagcat caaagcggtg gcatcccagg gcatatggcc ctggtgccat
     2041 tggataaacg ccagttgttg gccgttgggg gcgaggctta agtacgcgta aaagtctgcc
     2101 ccttgttgca gaactcttgg ggtttgttct tcaaggctga ggtcgatact tgcgatgcta
     2161 aggcttgaaa gcccgtcgct aggagtctcc atcaccgcca atattcggtt gcgcctagta
     2221 tcccactgaa tatcggcgta gcggccgccg ctttgggtga gcttgcgggg cgcagccggg
     2281 cgggcaaaat ccatggcata gaggttctga tcctgcgcgt tcccccagat cagctccttg
     2341 cctgccagcc aaaaagggca gccgccgtat tctaaaaact ggctgcgggc actgtgctcc
     2401 tccggcaata attcccgctt ggtgccatcg ggcaactggg ccacaataac ctggcggccc
     2461 ttttgcgctg gccgcgcctc caaccaaaac aaccatccac tagccccctg cgggtacatg
     2521 ggctgaattg cattggcgag gacggcatca acactaatag gggattccca aaatccgtaa
     2581 ggggcttgtt ttggcggcga ttgatggttt ggcattacag ggctcgtggc ggcatgatca
     2641 ggtagttaag gtttttaggg agtctatggc ggcttacggc ctggcacaag gtaacggggc
     2701 acaaggctat tgcgtagccc taagcggcgg gctggattct atggtgctgg cccatctggc
     2761 caaaacctat ttgccccagg tgcgcctgct gcacatcaac cacggcctct cgccatttgc
     2821 cgatcaatgg caacagcagg tggagcactg ggccgctgag cagaatctgc cctgccacag
     2881 tcaacgagtg ctggttgccc gccagggcga gagcctggag gccgccgccc gcaaggcccg
     2941 ttatgcggta tttgaagcaa ccctaagccc aggcgaagcc ctcttgtgcg gccatcactt
     3001 gaacgatcag gcggagacgt tttttctgcg cctattgcgc ggcagtggcc tggctggcct
     3061 aaccggcatg gccccggccc ggcccatcgg cgccggggtt ctgctacggc ctttactggg
     3121 gcttgagcgt ggcgcgctgc tagcctatgc ccaagcccag ggccttacct gggtggaaga
     3181 tgagagcaac agtgacgagc gctttgagcg caattggctg cgccatagcc tattgcccca
     3241 attaaccagc cgctggccca aggccgcagc ccaagtggcc aacacctgcc tgcgcctgca
     3301 ggaggagcag caattactgc aggagtattt agcctctgac cttgcctcct gtagccctcg
     3361 gcccgagcgc ttgggccaca gcctggattt aacctgcctg caacagggca gcagcgctcg
     3421 ccgcaacagc ctgctgcgcc tgtggt
//
LOCUS       DAOQGS010000452         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15785_length_3442_cov_5.237083, whole genome shotgun sequence.
ACCESSION   DAOQGS010000452 DAOQGS010000000
VERSION     DAOQGS010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15785_length_3442_cov_5.237083"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1149
                     /locus_tag="PKZ52_14520"
     CDS             280..1149
                     /locus_tag="PKZ52_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNG61035.1"
                     /translation="MPALNSLNHIIARIKDGTVVPYLGPDALRGVVDMLSKNPIPADS
                     DSLILAMNDGKPMAPRLMYEFARAAMNVEQKKGRKSVERFLTTTYGERKWSGSVFHQW
                     LAGLSPQYVIDINRDTQLQQLYADRAHTLIVGVARIAGTQYRFKLYQFNPGAGYAPIE
                     LEQVDKLLPVLFKPMGTPLPEANYIASDADYVDYITELMGGFAIPSFLKEYRLGKKYV
                     FIGMRFLRDTERMVLSDIIHGADSPAGFVLLPEPTDKERRFCDKKQLQIIEADWQSLV
                     AASSADQELAALA"
     gene            complement(1737..2459)
                     /locus_tag="PKZ52_14525"
     CDS             complement(1737..2459)
                     /locus_tag="PKZ52_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNG61036.1"
                     /translation="MSQIANLSREVALRIALAARILPDVGVSRLLEILHRRISDDLTE
                     ESLKLITVTDLKTGFASTDGEEDGEDISVGLPALKEAVRILWGENDQEQLPKPVELTE
                     PMPGSIRVAIASNSGETLNGHFGSCIRFLVYQVSATDYKLVGIQSAVEADLSEDRNLF
                     RVDLIRDCQVLYVVSIGGPAAAKVVRGGIHPMKVIEGGEASEIIAKLQAVMSNSPPPW
                     LAKILGVDPEERVRFERSEATG"
     gene            complement(2508..2771)
                     /locus_tag="PKZ52_14530"
     CDS             complement(2508..2771)
                     /locus_tag="PKZ52_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNG61037.1"
                     /translation="MEQAEIINIIKQALPSAQISAEGADCSFTVQVVSESFSGVGAVK
                     RQQQILGLFSALLTTGALHALSVKAYTPEEWAAAQTPLTQLSL"
     gene            complement(2892..3224)
                     /gene="grxD"
                     /locus_tag="PKZ52_14535"
     CDS             complement(2892..3224)
                     /gene="grxD"
                     /locus_tag="PKZ52_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016145530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNG61038.1"
                     /translation="MSTSPTVALIQQQLSENPVILYMKGTADHPECGYSGAAVAALKK
                     AGIAFSFVNVLQAPLIRERLPSISQWPTFPQLFVKGELVGGSDIVCAAVADGTLQAMV
                     AKAADVQA"
BASE COUNT          929 a          840 c          752 g          921 t
ORIGIN      
        1 attgaaggta tgactgcagc aggttattta ggctaaagct ataccacagg gcttaagctg
       61 aggttttgga ttaattcgat tcttcttccc aactattctc tgcagacggc atgtattggc
      121 tattaatggt ccgctgagcc tttgattgca gaaactgcca gcctttaatt aatccctttt
      181 atgccaccgg tttagggcat caacggtaaa ttctgtaatg gcgtgaattt tgcccctatg
      241 gattttttat tgtcgctttt tattaaccga ggaatctcta tgcctgcttt aaactcgctc
      301 aatcatataa ttgcccgcat taaagatggc acagtcgtcc cttatttagg gccggatgcg
      361 ctgcgcggcg tggtggatat gttgtctaaa aatccaattc ccgccgacag cgatagcctt
      421 attctggcca tgaacgacgg caaacccatg gcgccgcgtt taatgtatga atttgcacgg
      481 gcggccatga acgtagaaca aaaaaaaggc cgcaaatccg tggagcgctt tttaacgact
      541 acctacggtg agcgcaaatg gtctgggtcg gtgtttcatc aatggttggc cggtttatcc
      601 ccacagtatg taatcgacat caatcgcgat acacaactgc aacaactcta tgctgatcgc
      661 gcccatacct taattgtcgg cgttgcccgt attgctggca cccaatatcg atttaagctc
      721 tatcaattta atccgggcgc aggttatgcc cccattgaac ttgaacaggt ggataagctg
      781 ctgccggtat tattcaaacc catgggcacg cccctaccgg aagccaacta tattgcctcc
      841 gatgccgact acgtggatta catcactgaa ttaatgggcg gctttgccat tcctagtttc
      901 ttaaaggaat accgcctggg aaaaaaatac gtttttatcg gtatgcgttt tctgcgggat
      961 acagaacgca tggtgttaag cgatattatt cacggcgctg actcacctgc gggttttgta
     1021 ctcttacccg aacccaccga caaagaacgg cgcttttgcg acaaaaaaca actgcaaatt
     1081 attgaagccg actggcaaag tttagtggcc gctagcagcg ccgaccagga actcgcggcg
     1141 ctcgcctaga ttagcttagg ttaatggcag aaaaacaaat ccaatagggg ttggatgtgt
     1201 ttagggccta tcccatctgc gcacagtttt taaagatctt ttttactgcg ggcaatggca
     1261 taggctcatt ttttcatttt cgatcagatt ttcttgcagc atcttccagg cattgaatcc
     1321 cgccggatta atagcaaaca ctataatcaa tactcgaata aatattcgca ggaaattacg
     1381 aaaaaggccg ccttatctag acggcctttt ttaaagccga gaaactattt agctagcaaa
     1441 cttaagcatt cattaccgca gacattattt ccctttccat gcgaccggca aagcagcata
     1501 attaagccat accaaataat aaatccgcgg tatagcagtg gttaatacag gtcatttcag
     1561 accgtcaaaa agagggatcc ttttgacgag cctacttagg atgtattcac ggcgtgtctg
     1621 aattgacctg tatttactac tgcgggcttc cagcactcca gcactaaaga aatctcctat
     1681 ttgtttcaac aaacggaatt caatagccag taaattaaaa aaatggcgtt gagcgcttag
     1741 cccgtagctt cgctgcgttc aaaacgtaca cgctcttcag gatctacacc gaggatttta
     1801 gctaaccaag gcggtggaga attgctcatt accgcttgta atttggcgat tatttcactg
     1861 gcctcgccac cttctattac cttcatgggg tgaattccgc cgcgtaccac tttcgcagcc
     1921 gcaggtccac cgatggaaac cacatacaac acctggcaat cgcgaattaa atccactcga
     1981 aataaattgc gatcttccga taaatctgcc tctactgctg attgaatccc taccagctta
     2041 taatcggtag cgctgacttg ataaactaaa aagcgaatac aggacccaaa atggccatta
     2101 agcgtttcgc cactgttgga ggctatggcc acacgtatgg atcctggcat gggctcagtt
     2161 aattcaacgg gcttcggcaa ttgctcttgg tcgttttcac cccataaaat tcgcactgct
     2221 tctttaaggg ctggcaagcc tacgctaatg tcttcgccat cttcttcacc gtccgtgctg
     2281 gcaaagccgg tttttaaatc cgtgacggtt attaatttta aagactcctc ggttaaatca
     2341 tcgctgatgc ggcgatggag tatttctaac aaacggctca cacccacatc aggcaagatg
     2401 cgtgcggcca aggctattcg cagcgcaact tcccggctta aattggcgat ttggctcata
     2461 aggcacctcg ataaaaatgg atgaaagtct gtggccgtag atagttttta aagggacaac
     2521 tgcgttaatg gcgtctgcgc cgccgcccat tcctctggtg tataggcctt tacactcagc
     2581 gcgtgcaatg cacctgtggt cagtaatgca ctaaacaatc ccagtatttg ctgctgacgc
     2641 ttaaccgcgc ctacgccact gaaactttca ctgactacct gcacggtaaa actacagtca
     2701 gcgccctcgg cactaatctg tgcactgggc aaggcctgtt tgatgatatt aataatttcc
     2761 gcttgttcca tagttgatat acaccaaata ggattaaaaa ttatgcattg aacccaacat
     2821 gaatgctctg caaaaattca ccgcctaaaa aacgtcgcgc gccagcattg cgattcacta
     2881 ctaaaatgag cttacgcctg aacgtctgcg gccttagcaa ccatcgcctg taaggtacca
     2941 tcggccactg ctgcacagac tatatcgctg ccgcccacca gctcgccttt aacaaataat
     3001 tgtggaaagg taggccactg agaaatagag ggtaaacgct cgcgaattag gggcgcttgc
     3061 aatacattga caaagctaaa tgcaattccg gcctttttta atgcagctac agccgcgcca
     3121 gaataaccac actccgggtg atccgcagta cctttcatat ataaaataac gggattttcg
     3181 ctcagttgtt gctgaataag cgcgacagta ggtgatgtgc tcataaataa tttccaatgg
     3241 taagttatgt caattcatta agtgaaggta attttttagc cagcgcgagc taatggcata
     3301 ctttggtggc gcaaaaagct aagggtttct atgttggcgg caacggccaa cgcggtaaaa
     3361 tcatctaaag tagcgagaga cgtatcggca cctgcgtaga ccaggtgttt gacaatatta
     3421 atattcccaa cggatgcagc at
//
LOCUS       DAOQGS010000453         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15861_length_3433_cov_4.486382, whole genome shotgun sequence.
ACCESSION   DAOQGS010000453 DAOQGS010000000
VERSION     DAOQGS010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15861_length_3433_cov_4.486382"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKZ52_14540"
     CDS             complement(<1..673)
                     /locus_tag="PKZ52_14540"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HNG61039.1"
                     /translation="MGQLIWAVCLLVLSLRLYAAEPFYQVSTPVSSQAPEVRAQAASK
                     ALKDMLWRLTGSQQAEQNPALASIFSRAETYADSFSYTGSGQITFTFSPQGLNQLIKE
                     YGLPYWPANRPKVLVWSLVNGQWPAPGDSALAELKAQAQLRGIPLVFPNYDSEDRTAL
                     SLEQFKAFDSEALLNASFRYSLDTVMAIIISDSGSRWTFDHRGDKRSGDNPVQGINAL
                     ADFLAA"
     gene            820..1101
                     /locus_tag="PKZ52_14545"
     CDS             820..1101
                     /locus_tag="PKZ52_14545"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNG61040.1"
                     /translation="MLLDLTRHITQEITERLSALPKPPLPQLDQGLLRHWVEAAVQKC
                     QLVSREEFDAQTEVLLRTRLKVEALEAQLAQLEAKFDELSSAKNDSAHT"
     gene            1289..2002
                     /locus_tag="PKZ52_14550"
     CDS             1289..2002
                     /locus_tag="PKZ52_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNG61041.1"
                     /translation="MTETIRLSKKLIQQIGCSRREAELYIEGGWVTVDGEVIDQPQFP
                     VADQTVLLLPGANTTAAGPITLIMHVAADLNKEQTLAHLIAANRWAEDQTAGRLLKRH
                     LFQIKPCLPLLPGVAGMQILSQHWGLQRKFAEDGARMEQEFIVEVNGALPAEKLALLN
                     SGLSFLGKKLPPAKVSWQSETRLRFALKNPQPGQIQSVCEQVGLEVITIKRIRIGTTS
                     MGKMPLGQWRFLDARKNIG"
     gene            complement(2270..2728)
                     /locus_tag="PKZ52_14555"
     CDS             complement(2270..2728)
                     /locus_tag="PKZ52_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61042.1"
                     /translation="MRKMYGIPSFFILYALATAQHAHSCTGDEHVKYIKEVRFDNDKL
                     ACGSELKRLVIKWSSQAPKGSPKPNGIGLVVKNAKGKEIVRYDLPETKPDSLVYATCI
                     NKHYAPQTEIRFYFSEFTNTRTEKNGSVSQTFAINPKNESCVLSELLTTP"
BASE COUNT          837 a          839 c          891 g          866 t
ORIGIN      
        1 gggcggcgag aaaatccgcc agagcattaa tgccctgcac agggttatca ccactgcgct
       61 tgtcaccgcg atgatcaaag gtccagcggc tgccgctatc gctgatgata atagccatca
      121 ccgtatccag gctgtagcgg aaggaggcat tgagcagcgc ctcgctatcg aaggctttaa
      181 attgttccag gcttaacgcc gtgcggtctt cgctatcgta attggggaac actaagggga
      241 tgccgcgcag ctgtgcctgg gcttttaatt cggctagggc actgtcgccg ggggctggcc
      301 actggccgtt cacgaggctc cacaccaaca ccttgggccg gttggcgggc cagtagggca
      361 ggccgtactc cttgatcaac tggttgaggc cctgggggga gaaggtaaaa gtaatttggc
      421 cgctgccggt gtagctaaaa ctatctgcat aggtttcggc gcggctaaat atactggcta
      481 aggcggggtt ttgttcggcc tgctggctgc cggttaagcg ccagagcata tctttcagcg
      541 ctttactggc ggcctgggcg cgcacctccg gggcttggga gctaaccggc gtgctcacct
      601 ggtaaaaggg ttccgccgcg tagaggcgca ggctcaatac caataaacat actgcccaga
      661 tcaactgccc cacggcccgc cctctttatc gttaaaaagc gggcattgta gcggcaaata
      721 cctccagggc gtacaagggg cgggagccgt tggggttttg gtgtacactt tgcggccagc
      781 gcgccccgct ggcctgttcg accctttgaa ggtgctgcca tgttgcttga tttaacccga
      841 cacataaccc aagaaattac cgagcgccta agcgccttgc ccaagccccc gctgccccaa
      901 ttagaccagg gcctgctgcg ccattgggtg gaagccgccg tgcaaaaatg ccaattggta
      961 agccgggaag aatttgatgc ccagacggaa gtgctgctgc gcacccgttt aaaagtagag
     1021 gccttagagg cgcaactggc gcagctggaa gcaaaattcg acgagctcag cagtgccaaa
     1081 aacgattcgg cccacaccta atactccctc gttacctgcg tattattaac ccggcagctt
     1141 tgtatgccgg gttaataatt attgattaaa tatggccgct acagtacggc ggcgcattta
     1201 ggtttaatta gagcatcctt gattaaagtg attagagtgc ggtgaattct ccttccgccc
     1261 ctatctattc ctattattag agcgttccat gaccgaaacc atacgtttaa gcaaaaagct
     1321 tattcaacaa attggctgct cccgccgcga agctgagctg tatattgaag gcggatgggt
     1381 aaccgtagat ggtgaagtga tagaccagcc gcaatttccg gtggctgatc aaaccgtact
     1441 tttattgccc ggcgccaaca ccaccgctgc aggccctatt accttaataa tgcatgtggc
     1501 ggcggattta aataaagaac aaaccttggc gcacttaatt gccgccaatc gctgggcgga
     1561 ggatcaaacc gccgggcgtt tactaaagcg ccatttattc caaataaaac cctgcctgcc
     1621 cctgctgccc ggtgtggccg gtatgcaaat attaagtcag cactgggggt tacagcgtaa
     1681 atttgccgaa gacggcgcgc gtatggagca ggaatttatt gtggaagtaa acggcgcact
     1741 gcccgctgaa aaactcgccc ttttaaattc cggcttaagt tttttaggca aaaaattacc
     1801 ccccgccaaa gtgagctggc agagcgaaac ccgcctgcgc ttcgccctta aaaaccccca
     1861 gcctgggcaa attcaatcgg tatgtgaaca ggtgggcctt gaggtaatca ccattaaacg
     1921 catccgcatc ggcaccacct ccatgggtaa aatgccgctg gggcagtggc ggtttttgga
     1981 tgcaaggaag aatataggtt agttgtgttt gcccgaattt tttgcaagcg gtgtaaattt
     2041 ttagcgcgca gttaaataag gatttacaca tttaacgccg ccagcgccag caacaaaata
     2101 attcaacttc cgttaggggt tgttttattc actgtgtagc ccggatggag tgtagcgcaa
     2161 tccggggtct taaataatcc ctgattacgc tgcaatccat ccaggcaacc cagattagca
     2221 tttgcgaatg cgccagtcga aaggctgcga aggaaaagtg ctagcggttt tatggcgtag
     2281 tcaataactc gcttaaaaca caggattcat tcttggggtt aatggcaaag gtttgcgaga
     2341 cagaaccatt tttctcagtg cgggtgttgg tgaactccga aaaataaaag cgtatttcgg
     2401 tttgtggtgc ataatgtttg ttgatgcatg tggcatacac aagtgagtca ggttttgtct
     2461 ccggtaagtc atagcggacg atttctttgc ctttggcatt tttaactacc agtccaattc
     2521 cattgggttt tggtgatcct ttaggggctt ggctagacca tttaataact aagcgtttaa
     2581 gttcgctgcc gcaggctaat ttatcattgt caaaacgcac ttccttaatg tacttaacat
     2641 gctcgtctcc ggtgcagcta tgtgcatgct gcgcggtggc taatgcatag aggataaaaa
     2701 atgaagggat gccatacatt tttctcattt taaaacgtcc taattttgtg aattcggcga
     2761 catgctagcc caggcaaata caactgtaaa tatagcgctc ggacaggagt agggcggggc
     2821 ctggtaggct aacacttaac ggcgcgcact tttaagcgac ttttagaatg gtgttaatcg
     2881 agtgctaccc aggcgctaaa ttgtagcgtt tagcggcgag atccgggtat tcaattggta
     2941 ggcgcataga ggcttcttaa gtgtaatcat tcctatattc accactaaat ttgctgctag
     3001 acccgtaggg ctcaccttgg tgcaccgctc cgacggccgc agaaaatttt attaattaac
     3061 tctacgagtg cgatcgaggt gatgccgatc acaatttaag ggtttgttcc tgcttttgtg
     3121 gaggtgagta tggcgctacg tgtcttgcat ccaggtgatt gttagagggg atttaggact
     3181 tgcctctagt gctattgcca aaactcgcag cgagaacagc ccagggcaat ttctaacaat
     3241 ccctctgatg ggcgcgccgc tgctcgaaaa ccaaattaat aaactattac aggtaacgtc
     3301 atgaataaac aaatgactgg cttaatagca ttggcatgtg ggcttagcgc ctgtggcggc
     3361 gcgccgtcga taaactcgtc ttcatcatct tcatcatctt catcatcttc atcatcttca
     3421 tcatcttcat cat
//
LOCUS       DAOQGS010000454         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15920_length_3426_cov_4.746366, whole genome shotgun sequence.
ACCESSION   DAOQGS010000454 DAOQGS010000000
VERSION     DAOQGS010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15920_length_3426_cov_4.746366"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKZ52_14560"
     CDS             complement(<1..562)
                     /locus_tag="PKZ52_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNG61043.1"
                     /translation="MVAASINFEHWADFLFQLGAISGASEFQGFLVGLQVSGEPLAED
                     AWQDTVAQFLDIAMLPKTPEQLAGLTALNAMVSHALADGDYRFRLLLPDDALPLAARL
                     EALAQWCQGFLFALGHAGAEGVKQEGLDDVAQIAQLEHSCDESEENEVYFAELVEYVR
                     VVVLQLAAEQARRTASAAPLQAPTDAH"
     gene            782..994
                     /locus_tag="PKZ52_14565"
     CDS             782..994
                     /locus_tag="PKZ52_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HNG61044.1"
                     /translation="MQDSLTAQLAAQLDRLLHEYAALKAENAQLKQAAQHWQQERQLL
                     IDKNTLACNRIEAMIARLKKWDGPHD"
     gene            987..1286
                     /locus_tag="PKZ52_14570"
     CDS             987..1286
                     /locus_tag="PKZ52_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNG61045.1"
                     /translation="MTKPHRVTLTLLEKDYQIACPPQEQAALLTAAKEVEARMRSTRV
                     QQGVVGSERIAVLVALNLCYELQQLKTQQAEGGHRADDEALQQMLVKLQAALDGA"
     gene            complement(1298..2140)
                     /locus_tag="PKZ52_14575"
     CDS             complement(1298..2140)
                     /locus_tag="PKZ52_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG61046.1"
                     /translation="MGLTVLVVDDATFIRDLVKRAVRQMVGEVELLEAADGARAQALI
                     KQKAPDLILSDWEMPEVSGEELLKWVRAQPNLVATPFIMITSRGDRNHVIAAVNAGVS
                     DYISKPFTAEELSRKVHKQLKRIGFSPKAPAAKPAGAFSSLEVLTGATAERSPKAAPK
                     PAVAAAFAKPVAAPSAAPAKVTLQAHLRLAKQAFVCEVKEISLQAMSGLIDRTETLPQ
                     LFEVAALDILDAQGQVLARLNVYIHALTAASLSPESDKVRVMVRFVDQDPAKLAALSQ
                     WIQR"
     gene            complement(2196..2978)
                     /gene="yaaA"
                     /locus_tag="PKZ52_14580"
     CDS             complement(2196..2978)
                     /gene="yaaA"
                     /locus_tag="PKZ52_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNG61047.1"
                     /translation="MLALLSPAKDLDFDSPILDIKATQPRLPEHSKDLMQTLKQAQVA
                     DVAALMHLSEKLALLNYTRFQDWRWPQPKRRARQAIWAFNGDVYQGLQARSWAAEDVA
                     FAQSHVRILSGLYGLLRPLDAILPYRLEMGTSLATERGRDLYQFWGETVTQLLNKDLK
                     AEGSGLVVNLASQEYFKVVKPKHVAGRIIEPVFKDYHAPSGTYKIISFFAKKARGQMC
                     AYIVNNRLTQADELKSFSGDGYGYNAALSSGDTWVFTRRAEA"
     gene            complement(2997..3425)
                     /locus_tag="PKZ52_14585"
     CDS             complement(2997..3425)
                     /locus_tag="PKZ52_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HNG61048.1"
                     /translation="MVERLQVQVNEQNECQLVRSPAFIKGLASGDVIKLTGATDGAPQ
                     FELVRRSGNLAIRVVSRGDIQALSERLTPHLEQLGGELDAETPRLLIYSIHVSLGFTA
                     IEALLNGAMDEHSAWFYGNVYDPADGTTPLNWWLDVLDQV"
BASE COUNT          717 a         1015 c         1004 g          690 t
ORIGIN      
        1 agtgggcatc ggttggggct tgaagtggtg cggcagaagc cgtgcggcgc gcctgttcgg
       61 cggccaattg caacaccacc acccgcacgt attccaccag ctcggcaaaa tacacttcgt
      121 tctcctcgct ctcgtcgcag ctgtgctcta gctgggcaat ctgcgccaca tcgtccaggc
      181 cctcctgttt cactccctct gcgcctgcgt ggccgagggc gaacaaaaag ccctggcacc
      241 actgggcgag ggcttctaag cgcgccgcga gcggcagggc gtcgtcgggc agcagcaggc
      301 gaaaacggta gtcaccgtcg gccagggcgt ggctcaccat ggcgttgagg gcggtgaggc
      361 ctgccagctg ttcaggggtt ttaggcagca tggcaatgtc caaaaactgc gccactgtgt
      421 cctgccaggc gtcttcagca aggggttcgc cgctcacctg caggcccact aaaaagcctt
      481 gaaattccga ggcgccgcta attgccccga gctgaaataa aaaatctgcc cagtgttcaa
      541 aattgatgct cgctgcgacc atggccctgc ctgctaaaac gcggaatgaa acaatataag
      601 ctctgcccca catgctacca taagccttat aaattaaggc acttaaggct cgctggcacg
      661 gggctcgcag gcacttcacc agccgctgca cgctacctag gtgcggccct ttctttgtac
      721 gttttgccta aggggtttag ttgatgttca gtgcccatcc accggccagg tcataacgct
      781 tatgcaggat tcgctcaccg ctcaattggc cgcccagctg gatcgcctgc tgcacgagta
      841 cgcggcgctc aaagccgaaa acgcccaact aaaacaagcg gcgcaacact ggcaacagga
      901 gcgccaactt ttaatcgaca aaaacaccct cgcctgcaac cggattgaag ccatgattgc
      961 gcgcctgaaa aaatgggacg gcccccatga ctaaacccca ccgcgtcacc ctgaccctgc
     1021 tggaaaaaga ctaccagatc gcctgcccgc cccaggagca ggccgccctc ttaacggcgg
     1081 cgaaagaagt ggaagcccgt atgcgcagca cccgtgtgca acagggggtg gtgggatctg
     1141 agcgcattgc ggtgctggtg gccctcaacc tctgttacga actgcagcag ttgaaaacgc
     1201 aacaggctga gggcggccat agggccgatg acgaggcact gcaacagatg ttggtaaagc
     1261 tgcaggcggc tttagacggc gcctgagaag gcccggccta gcgctgaatc cactgcgaaa
     1321 gagccgcaag cttggccggg tcctgatcca caaagcgcac catcacccgc accttgtcac
     1381 tctccgggct caggctcgcc gccgtgaggg cgtggatata aacattcaag cgggccagca
     1441 cctggccctg ggcatccaga atatccaggg ccgccacctc aaataattgc ggcaaggttt
     1501 ccgtgcggtc gattaggccg ctcatggcct gcagggaaat ctcttttact tcgcagacaa
     1561 aagcctgttt agccaggcgc agatgggcct gtagagttac tttggcgggg gcggcgctcg
     1621 gcgccgccac aggtttggca aaggcagccg ccaccgcagg cttaggggcg gcctttgggc
     1681 tgcgctcggc ggtggcgccg gtgagtacct ctaaggagct aaaggcccca gcgggtttgg
     1741 ccgccggagc cttggggcta aaaccgatgc gtttgagttg tttatgtact ttgcggctga
     1801 gttcttcggc ggtaaaaggc ttggagatgt aatcgctgac cccggcgttg acggcggcga
     1861 tcacatgatt gcgatccccc cggctggtga tcatgatgaa gggcgtggcg actagattgg
     1921 gctgggcgcg cacccatttg agcagctctt caccggagac ctcgggcatt tcccagtcgg
     1981 atagaatcaa atccggcgct ttttgtttga ttagggcctg ggcgcgggcg ccgtcggcgg
     2041 cttctaataa ttcaacctcc cccaccatct ggcgcacggc gcgtttgacg aggtcgcgaa
     2101 taaaggttgc gtcgtctacc actaacaccg ttaagcccat tgccctgtac cctgttcatg
     2161 gaagctaact gccctgttat ccagatgcgc ctgggttatg cctcggcgcg gcgggtaaac
     2221 acccaggtgt cgccactgga tagggctgcg ttatagccgt acccatcgcc gctgaaactt
     2281 tttaactcat cggcctgggt gaggcgattg ttaacaatat aggcgcacat ttggccccgc
     2341 gcctttttcg cgaaaaaact tatgatcttg taggtgccag aaggggcgtg gtaatcctta
     2401 aacaccggct caataatgcg cccggccaca tgtttgggtt taaccacctt gaagtattcc
     2461 tgggaagcga ggttcaccac caatccgctg ccctcggctt ttaaatcctt gtttaacaac
     2521 tgggtgaccg tctcccccca aaactggtag agatcccgcc cccgctcggt ggcaagacta
     2581 gttcccatct ccaagcggta gggcagaatg gcatccaggg gccgcaacaa cccatacagg
     2641 ccggagagaa tgcgcacatg gctctgggca aaggccacgt cctcggcagc ccaactgcgc
     2701 gcctgcaggc cttggtagac atcgccatta aacgcccaga tggcctggcg ggcccggcgt
     2761 ttgggctggg gccagcgcca gtcttgaaag cgcgtgtagt tgagcagggc gagtttttca
     2821 ctcaggtgca taagcgccgc cacatccgcc acctgggcct gtttgagcgt ttgcataaga
     2881 tccttgctgt gctcaggcaa gcgcggctgg gtggctttga tatcgagaat cggcgaatca
     2941 aagtctaggt ctttggcggg ggagagaagg gcgagcatgt ttatacctgt ttgaatttac
     3001 acctgatcta aaacatcgag ccaccagtta aggggggtgg tgccatcggc gggatcgtag
     3061 acattgccgt agaaccaggc gctgtgttca tccatggcgc cgtttaacag ggcttcgatg
     3121 gcggtaaacc ccaggcttac atggatgctg taaatcaaca ggcggggagt ttcggcatcc
     3181 agctcaccgc cgagttgttc caaatggggg gttaggcgct cggaaagcgc ctgaatatcc
     3241 ccccggctca ccacccggat ggccaaattg ccgctgcggc gcaccagctc aaactgcggt
     3301 gcgccgtcgg tggcgccggt gagtttaatc acatcgccgc tggcaaggcc cttgataaac
     3361 gccggcgagc gcaccagctg gcactcgttc tgttcattca cctgcacctg taggcgctca
     3421 accacc
//
LOCUS       DAOQGS010000455         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15961_length_3420_cov_3.604458, whole genome shotgun sequence.
ACCESSION   DAOQGS010000455 DAOQGS010000000
VERSION     DAOQGS010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15961_length_3420_cov_3.604458"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKZ52_14590"
     CDS             complement(<1..517)
                     /locus_tag="PKZ52_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HNG61049.1"
                     /translation="MLKPFIFASVSLLGSVVASQAWAAKTVDVLVLYGSSAQASRNGA
                     DINARIASYVAYANQAYASSGVDMQLRLVAAQKLDLPISNVSEANLNTLRQNATVAQL
                     RRQYGADVVSYLTLSQTVSGGSICGIGYIPAGNPTSATFFSDAPSLAFNLVGVDCNLN
                     VLTHEVGHNMGL"
     gene            complement(637..1140)
                     /locus_tag="PKZ52_14595"
     CDS             complement(637..1140)
                     /locus_tag="PKZ52_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61050.1"
                     /translation="MAASGLIPIPEPLPQVPWSGPARLVLWDNKSPPVTAQQDGIEQI
                     LVQLDPTHLASLHPGQILVMPLPDGAPEVHALITDTFNDATGTHNWRASVQNDLPNAS
                     VLITQGTEQTHIAIFTEQGSYTLIADNKTGKATLVDEGKLIARQALVDDGVVLHEHPE
                     LTPPLSP"
     gene            complement(1200..1358)
                     /locus_tag="PKZ52_14600"
     CDS             complement(1200..1358)
                     /locus_tag="PKZ52_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61051.1"
                     /translation="MRIVALVAGSLMFFVAVSYFLHERATGDVKPTNPMGTAQVIDVS
                     DNLKKKLS"
     gene            complement(1813..>3420)
                     /locus_tag="PKZ52_14605"
     CDS             complement(1813..>3420)
                     /locus_tag="PKZ52_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61052.1"
                     /translation="DSGVRLSVKLSWGETPEQSIEHKIATSKKWKTRSFKFKPGVNTT
                     SARFEILASGEGRCGIGAVSLMPADNIKGFRADTLALMKEMNCPILRMPGGNFVSSYD
                     WKHTIGDQDLRPPIYDPVWRAVQPNDVGIDELLQLCELIGSEPFWCASTGFAEPRSGA
                     ELVEYINGPADSTWGRVRALNGRKKPYGVKYWAVGNEMYGHWQMGHMAPEQYTIKHNL
                     FADAMRAVDQSIYIVAPGGFVDEMTTGQGIFIAGQPEVKVGSERDWAYSMFKGSWGKF
                     DALGTHAYPPQDKRFDLKTGKLFDVKMPLNEWARQPANRVRTMVDAWEEYKKHFPKLN
                     EGSVKVFFDEWAYGFEDSLKNCLAIALTLHEFFRHTDFIAMAGFTMATAWLDFNRTDA
                     TISAKGRIFQLYHQHFGAIPVAVSGTNPPPKPQYPIGGDQPSVNTGSLNWPLDVSASL
                     TEDKQALIVAVVNATEQAQLLELDVQGFTAAAKGRCWKFTGASLNARNAVGKPPEVLT
                     RETEFDARTRPILIAATSVEFYRFERA"
BASE COUNT          895 a          882 c          784 g          859 t
ORIGIN      
        1 caagccccat attgtggccc acctcgtggg taagcacatt taaattgcaa tccacaccca
       61 ccaaattaaa tgccaaactc ggtgcgtcac tgaaaaatgt tgcggaggtt gggttgcccg
      121 ccgggatata tccaatgccg caaatactac cgccggagac agtttggctg agggttaaat
      181 aagacaccac atcagcgccg tattgcctac gcaattgtgc aaccgtggca ttttgccgca
      241 aggtatttaa attcgcctct gaaacattac taataggtaa atctaacttc tgcgctgcca
      301 ctaatcgcag ctgcatgtct accccactgc tggcataggc ttggttagcg tacgccacat
      361 aagaagcaat acgcgcattt atatcggcgc cattgcgcga ggcctgtgca ctggatccgt
      421 ataatactaa tacatccacc gttttagcag cccaggcctg actggcaacc acacttccca
      481 acagacttac cgaggcaaaa ataaacggtt tcagcataat actatctcaa cataaacaaa
      541 ttttttgtag ccagtaaacg ctgtaaccag gcgcttttag gcgatactca cagccaacca
      601 cacaggcgaa ttaaaaccca ataaaagcat tcaaaactac ggcgataggg gtggtgttaa
      661 ttcagggtgt tcatgcaata ccacgccgtc gtccaccaac gcctggcgcg caattaattt
      721 accctcgtcc accaaggtag ccttgccggt tttgttatcg gcaattaatg tgtagctacc
      781 ctgttcggta aaaatagcaa tatgggtttg ctcagtgccc tgggtaataa gcaccgatgc
      841 attaggcaaa tcgttttgta cactggcccg ccagttgtgg gtgccggtgg cgtcgttaaa
      901 tgtatcggta attaatgcgt gcacctctgg cgcgccatcg ggtaacggca tcaccaaaat
      961 ttgcccgggg tgtaaggatg ctaaatgggt tggatcaagc tgcaccaaaa tctgctctat
     1021 gccgtcctgc tgcgcagtta ccggcgggga tttattgtcc cacagcacta agcgcgcagg
     1081 cccgctccag ggtacctggg gcaaaggctc aggtatgggg attaatccac tggcagccac
     1141 tgcaacgctg gtatttactg cggcagactt agctgaactg gctggcggcg cggcagaact
     1201 cagctcagct ttttttttag gttgtcgctc acatcaatta cctgggctgt acccatggga
     1261 ttggttggct taacatcacc ggtagcgcgt tcgtggagga aataactaac tgccacaaaa
     1321 aacatcaagc tgcctgcgac caaagctact atacgcacac accctccgat caatcgtaat
     1381 atttgctgcc tattgctaga aggatagaag gataaatcct aggcaaagga tcgttttatt
     1441 ctaaatcgaa gccaaaactg cgctcttggt tttaatcgag ctgggttggc tgatgctgtg
     1501 tgaaaattca tcattgccaa acggatatag gattttgaac ttttgaacga ttcattaacc
     1561 cggcggcatt tagtgccggg ttaataatgg cggcaaggat gccgcagagg cggcgcaagc
     1621 cgccgcgagc cgcgctaaaa caagaaaatc catgtatttt tgccagtttt agcgcagcga
     1681 aggaaaagta gatcaggacg atctattttt ccgccgggtt aatagtaaag atgatctgat
     1741 taatcctaag ttaatacaaa aatcccggcg cctatatatc aacgccgggg cggattaaat
     1801 atctgcgtgt tattaggccc gctcgaagcg ataaaactcc acactggtag cggcgattag
     1861 tatcggccta gttcgagcgt caaattccgt ttcgcgcgtc aacacttctg gcggcttacc
     1921 caccgcgtta cgcgcattta aacttgcccc tgtgaatttc caacagcggc ccttggctgc
     1981 cgcagtaaag ccctgtacat ccaactctaa taactgcgcc tgctctgtgg cattaaccac
     2041 cgcaacaatc aaagcctgtt tatcttccgt gagcgatgcg gatacatcca acggccaatt
     2101 gaggctgccg gtattaacac tgggctgatc accaccgatg ggatattggg gttttggcgg
     2161 tgggttggta ccgcttacag caacaggaat cgcaccaaaa tgctgatgat acaactgaaa
     2221 tattcgccct ttggcgctta tggttgcatc ggtacggtta aagtccaacc aagcggtcgc
     2281 catagtaaaa cccgccattg caataaaatc ggtatggcgg aaaaattcat gcagtgtcag
     2341 cgcgatggct agacaatttt ttaaactgtc ctcaaaacca taggcccact catcaaagaa
     2401 aactttaaca ctgccttcgt ttaatttcgg aaaatgtttt ttatattcct cccacgcatc
     2461 taccatggtg cgcacacgat tggctggttg gcgcgcccat tcattcagcg gcatttttac
     2521 atcaaataat ttgccggttt ttaaatcgaa gcgtttatct tgtggcggat aagcgtgcgt
     2581 gcctaaggca tcgaatttac cccaactgcc tttaaacata ctgtaggccc aatctcgttc
     2641 agaacccact tttacctctg gctgaccggc aataaaaata ccttggccgg tggtcatttc
     2701 atccacaaaa ccaccggggg ccactatata aatagattgg tccaccgcgc gcatggcatc
     2761 ggcaaataaa ttatgtttaa tggtgtattg ctccggtgcc atatggccca tttgccaatg
     2821 gccgtacatt tcattgccca ccgcccaata tttaacgccg tagggttttt tgcgaccatt
     2881 taatgcgcgc accctacccc aagtgctatc ggcagggccg ttaatatatt ccaccaattc
     2941 cgcacccgag cgtggttcgg caaacccggt gctggcacac caaaacggtt cgctgccgat
     3001 aagctcacac aattgcagca gctcatcaat acccacatca ttgggttgca cagcgcgcca
     3061 cactggatcg taaataggtg ggcgtaaatc ctggtcacca atagtgtgtt tccagtcgta
     3121 ggaggacaca aaattaccgc cgggcatccg caatatgggg caattcattt ctttcattaa
     3181 tgccagggta tccgcgcgaa agcctttaat attatcagcg ggcattaaag agactgcgcc
     3241 aataccgcag cggccctcac cgcttgcgag aatttcgaac cgagcagaag tggtattcac
     3301 accgggttta aatttaaaac tgcgggtctt ccatttcttc gaagtggcaa ttttatgttc
     3361 aatactttgc tcaggtgttt ccccccaact gagttttaca ctgagcctta ctccagaatc
//
LOCUS       DAOQGS010000456         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15972_length_3418_cov_5.314897, whole genome shotgun sequence.
ACCESSION   DAOQGS010000456 DAOQGS010000000
VERSION     DAOQGS010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_3418_cov_5.314897"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1353)
                     /locus_tag="PKZ52_14610"
     CDS             complement(175..1353)
                     /locus_tag="PKZ52_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61053.1"
                     /translation="MIKVFAIGNYRSLKNIVAPMGGLNLVTGANGSGKSNLYKALRLL
                     SDVAKGNLIATLASEGGLAYTYWAGPNSFSKDMKAGDAPIQGNARKANSRLHFGFVAE
                     GFGYSISLGLPPPPDPDDPEPSMFNLDPEIKRECIWAGDYYRPAACLVDRAGPMVKVR
                     AGKKWQVYTQHLNTYESMLSEIGDPIATPEIHQIRHIIRSWRFYDQFRTDAHSIIRNP
                     QIGTRTPVLDQDGHNLVAALRTIYEIGDRDGLFEAIADAFPGGSIGFHADSENRFTLE
                     FYQQGLLRPLKQAELSDGTLRYLLLIAALLTPRPPSLLVLNEPETSLHPDLLPALGRL
                     ISRVAKQTQVWVVSHASRLVANLEDNSDCNSIHIAKELGETHICGQGLLDAPPWYWPS
                     "
     gene            complement(1584..2198)
                     /locus_tag="PKZ52_14615"
     CDS             complement(1584..2198)
                     /locus_tag="PKZ52_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61054.1"
                     /translation="MKSSEAWKDFEEVKRLNLQLVKFENMQQKTAVLLLVRVTELCSE
                     TMLLVNQGCICSAPIILRSALESYIDMRCVIKSERHLDEMRGSFQGYQSKKEGKKRNL
                     QPMSIESKFKFAGKLEAYRGIYADLCLSAHGSIAIAVADHAAGNDVSVGHEADPVRKR
                     LYLNHIIFLASTALIECLTFLGFIDSQEEGLIKIKQNAEQGLYE"
     gene            complement(2814..3194)
                     /locus_tag="PKZ52_14620"
     CDS             complement(2814..3194)
                     /locus_tag="PKZ52_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61055.1"
                     /translation="MNECIIKLVSIFCLYAAVGGAVAVEAAPFLEKVSSSSNHKVFSC
                     SVGNAVAESGCMDGFETAVHTGGSSLNSTFINSSLSASFDSIQGHLKSNNYQIAPYCV
                     EVCVIYTDGRKGRCYWRCAFGYEG"
BASE COUNT         1008 a          833 c          687 g          890 t
ORIGIN      
        1 acgtgcgtgc acgcggtgct taagatggta aatagaaaca ataatccaca gcgaaatggg
       61 tacattctta agtcctaaaa tatattaatt aagataatta atggaaggga atactcgtgc
      121 aaaattgtaa catcgcgcag caataccttt aaacgtgctg acagatcgcc aatgttaact
      181 cggccaatac caaggcggtg cgtctaatag gccctggcca caaatatgcg tttcacccag
      241 ctctttggct atgtgaatag aattgcaatc gctattatct tccaaattgg caaccaaacg
      301 cgaggcgtga gataccaccc acacctgggt ttgtttcgcc accctgctaa ttaaccgccc
      361 cagtgcgggc aataaatctg ggtgcaggct ggtttctggt tcatttaata ccagcaatga
      421 gggcggccta ggagtcaata acgcggcgat taataataaa tacctaaggg tgccgtcgga
      481 taattccgct tgtttaagtg ggcgcaacaa accctgctgg taaaattcca acgtaaaccg
      541 attttcactg tcggcgtgaa agccgatact gccgccggga aaagcatccg cgatggcttc
      601 aaacaacccg tccctatcgc caatttcata aatagtgcgc agggccgcca ccaggttgtg
      661 gccgtcttgg tctaatacgg gggtgcgcgt gccgatctgt gggtttctaa taatagaatg
      721 tgcatccgtg cggaattgat cgtaaaagcg ccaactgcga ataatatgcc ttatttgatg
      781 aatctccggc gtggcaattg ggtcgccaat ttcgctgagc atgctttcat aggtgtttaa
      841 atgctgggta tacacttgcc attttttacc tgcacgcacc tttaccatgg ggccagccct
      901 atccactagg caagcggcag gccggtaata atcccccgcc caaatacatt cgcgtttaat
      961 ttcggggtct aaattaaaca tggatggctc tgggtcatcc ggatctggtg gaggcggcaa
     1021 ccctaaagaa atagaatacc caaacccttc cgccacaaaa ccaaaatgca ggcgcgaatt
     1081 ggctttgcgc gcattaccct gaatgggcgc gtcacctgct ttcatgtctt tactaaatga
     1141 attggggcca gcccaatagg tgtacgctaa accaccttca ctggccaaag tggcaattaa
     1201 attgcctttg gccacatccg ataaaagccg caacgcttta tataaatttg atttaccact
     1261 gccattcgcc ccagtgacaa gattaaggcc gcccataggg gcgactatgt ttttaaggga
     1321 gcgataattg cctatggcaa acacttttat catttattct tctccttggg caaaaacgag
     1381 cagcaagagt ttgtcgctgt ctaactcagt tacattaatt aactattgcc gcaggtaaat
     1441 tcgcagggta gatttggagc gaagcgacaa tccaccattt cgtggccccg gtctcgccgt
     1501 tcaatagaat aatgcttggc tgcaagaccg atggtggatt gctgcgcgaa tccgccctac
     1561 gattgatcca gcttacgggt tggttattca taaagcccct gctctgcatt ctgctttatc
     1621 ttgataagcc cctcttcctg tgaatctata aaccctaaaa acgtcaaaca ctcaatgaga
     1681 gccgtagaag cgagaaaaat aatgtggtta agataaagtc ttttgcgtac agggtccgct
     1741 tcatgaccta cagatacatc gtttcctgca gcatgatctg caactgcaat tgcaatgctt
     1801 ccatgtgcac ttaaacataa atctgcataa atacctctat atgcttccag ctttccagca
     1861 aatttaaatt tgctttcaat actcattggc tgcaaatttc gtttcttgcc ttcttttttc
     1921 gactgatagc cttggaatga accgcgcatt tcatccaagt gacgttcaga tttaataacg
     1981 cacctcatat ctatatagga ttctagcgcg cttcgaagaa ttataggggc cgagcagata
     2041 cagccttgat tgaccaacaa catggtctca gaacaaagtt cagtaaccct aaccaacaaa
     2101 agcacagcag tcttttgttg catattttca aatttaacca attgcaaatt taaccttttc
     2161 acttcttcaa aatccttcca tgcctcactg cttttcatct cgaatcctcg catactaatg
     2221 ccaatttaca gagcccagat tgccccctca atgggtagga gactttggaa aacagagcat
     2281 atgccagctc aagatctcta ccccagccat aacccctttg attgtaaaaa atacgtccca
     2341 ccagctaaaa atcacgacaa taggacatga aattcttttt tgggtaatac aataatttgg
     2401 ttaataaatt gagataggaa aacgcagggg gggggattcg gagcgaagcg aaaatacaca
     2461 atatcgcgac tcggatatcg ctgttcacaa taacaattcc ctgctgagag caaaatggtg
     2521 gattgctgtg aaaatccacc ctacgattga tccaccttac ctgttaagac tgatctacct
     2581 tacgcgtttg caagtcagga cttatatatg caattcgcaa gtgggcgcgg ggccaacaaa
     2641 gcagtgagca aataaaacgg ttaaaaccag acgctcaacc agtcaaatga acgcatcgct
     2701 gacacagaat aaaccaccaa acgtaactta atccaattac cacttaaaat agcgaaagat
     2761 taagttaaaa atatttggcg atcctatcta tgattcaatg accgagcatt ccgctaccct
     2821 tcgtatccaa atgcacagcg ccaataacac ctaccttttc ggccatccgt atagataacg
     2881 caaacctcca cacaatatgg tgcaatctgg taattattac ttttcaaatg accttgaata
     2941 ctatcgaatg aagcgctcag cgacgagtta atgaatgtgg aatttaagga tgagcctccc
     3001 gtatgaacag ccgtttcgaa gccatccata caccctgatt cagcaaccgc attaccaaca
     3061 gagcaggaaa acactttatg atttgaagaa cttgaaactt tctctaaaaa aggtgccgct
     3121 tcaactgcaa ccgcgccgcc cactgcagca tatagacaaa aaatagatac caatttaata
     3181 atgcattcgt tcatgacctt tcccttaaag ttgctcgttg caaagcccaa atcctattag
     3241 cattacattt attggtcaag ttttatctat tcaggttatg gataccaaga ccgaccttcg
     3301 ttaaaccaag caacctcgat gaccgcaaaa acgggccttt accgatggca tgtaggatgg
     3361 gagtaatacc cagcaattct acattttaat gttgggcata actgcccaac ctacccca
//
LOCUS       DAOQGS010000457         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_15974_length_3418_cov_4.230746, whole genome shotgun sequence.
ACCESSION   DAOQGS010000457 DAOQGS010000000
VERSION     DAOQGS010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15974_length_3418_cov_4.230746"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKZ52_14625"
     CDS             complement(<1..379)
                     /locus_tag="PKZ52_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61056.1"
                     /translation="MSESIRLVNQQLAFCQAQLLSARTSAGFNQRCTLQACKLQMHVL
                     MQAYLAEIAERHGLKWQIKLADLTESLAQLHNLLEQKTLVSADFNHLQQLINEPAAWR
                     YWLQLYQSALTAGTQTPLSPVLSD"
     gene            complement(398..3031)
                     /gene="topA"
                     /locus_tag="PKZ52_14630"
     CDS             complement(398..3031)
                     /gene="topA"
                     /locus_tag="PKZ52_14630"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNG61057.1"
                     /translation="MGKSLVIVESPAKAKTINKYLGSQYVVKSSIGHIRDLPTSGSGE
                     AVDPKARAAQAAQTRKMSAEEKVSYKAKKGREQLVKRMGIDPDNNWQAHYEILPGKEK
                     VIEELRKLAADADQIYLATDLDREGEAIAWHLREAIGGDESRYRRVVFNEITKSAIQE
                     AFKNPGRVDMNRVNAQQARRFLDRIVGYMLSPLLWEKIARGLSAGRVQSVAVRLISER
                     EKEIRAFIPEEYWDVTAELDTAKQERVNFSVVRYQGASFEPKTQAEAQKAVQAVKQSA
                     LAVIAREDKPTSSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTD
                     STNLSAEAVENCRAYIEITYGKQYLPEKPVVYSSKEGAQEAHEAIRPSEVTRLPSQLT
                     DMERDAERLYALIWQQFVACQMPPAQFTSTSVIAEAQGYQLRAKGRVVKFDGYLKVLP
                     AVAKKDDEGVLPDMKVGQALSLHSVQGNQHFTKPTARYTEASLVKELEKRGIGRPSTY
                     ASIISTIQERGYVHVQNRRFYANKMGDIVTERLHESFEDLMDYSFTANMEQNLDVVAE
                     GKKDWISVLNEFYKGFSNKLAKASGAEFGMRGNDPTETNIPCTTCGRNMQIRTGTTGV
                     FLGCSGYALPPKERCKTTMNLAPGEEAINTEDEEVELAQLRAKKRCKLCNATMDSYLL
                     DETRKLHICGNNPDCSGYEVEKGNFKLKGYDGPLIECDKCGADMQLKTGRFGKYFGCT
                     NEACKNTRKLLRNGEAAPPKMDAIPLPDLRCEKVNDYYVLRDGAAGLFLAASGFPKNR
                     ETRAPLLEEILPHKDQIDEKYEFLWSGPTRDPNGLPAIVRFSRKSKEHYLQTEIDGKP
                     SGWRAVYENGKWQEITGKAKA"
BASE COUNT          664 a          841 c          974 g          939 t
ORIGIN      
        1 catcgctcag cactgggctt aaaggtgttt gggtgcctgc agtaagcgcg ctttgataca
       61 gttggagcca atagcgccaa gcggcgggtt cattaattaa ttgttgtaga tggttaaaat
      121 ccgcgctcac tagagttttc tgttcaagta gattgtgcag ctgcgctaag gattccgtta
      181 aatcggctaa tttaatctgc cattttagcc cgtgccgttc ggcaatttcc gccagataag
      241 cctgcattaa gacatgcatt tgcagtttgc aggcttgcaa tgtgcagcgt tggttaaacc
      301 cagcgcttgt gcgtgcgctg agtaattggg cctggcaaaa ggccagttgt tggttgacta
      361 ggcgtatcga ttcactcata gcatgtgccc tgagggctta agccttggct ttgcctgtga
      421 tctcttgcca tttgccgttt tcgtacaccg cgcgccagcc tgagggttta ccgtcgattt
      481 cggtttgcaa ataatgttct ttgcttttgc ggctaaagcg cacaatggcg ggtaggccat
      541 tggggtcgcg ggtggggccg ctccagagga attcgtattt ctcgtcgatt tgatccttgt
      601 ggggcagaat ttcttccaac agcggcgcgc gggtctcgcg gttttttggg aaaccgctgg
      661 cggcgaggaa caaaccggcg gcgccatctc gtaacacgta ataatcgttg actttttcgc
      721 agcgtaaatc cggtagggga atcgcatcca ttttaggtgg tgccgcctcg ccgttgcgca
      781 ataatttacg cgtgttttta caggcttcat tggtgcagcc aaaatactta ccaaaccggc
      841 cggtttttaa ttgcatatcg gcgccgcatt tatcgcactc aattaagggc ccgtcatagc
      901 cttttaattt aaagttgcct ttttccactt catagccgga gcaatctggg ttattgccgc
      961 agatgtgtaa tttgcgggtt tcatccaaca aataggaatc catagtggca ttgcaaagtt
     1021 tgcagcgctt tttcgcccgc agttgcgcta actccacctc ttcatcttca gtgttaatag
     1081 cctcttcgcc gggagctaaa ttcatagtgg ttttgcagcg ctctttaggg ggcagcgcgt
     1141 agcctgagca gcctaaaaat acccccgtgg tgccggtgcg aatttgcata ttgcggccgc
     1201 aggtagtgca gggaatattc gtctccgtag ggtcgttgcc gcgcatacca aattcggcgc
     1261 cggaggcttt ggccaacttg ttggaaaagc ctttgtaaaa ttcatttaat acgctaatcc
     1321 aatctttttt gccctcagcg actacgtcta ggttttgttc catattggcg gtgaagctgt
     1381 aatccatcag atcttcaaaa ctttcgtgca ggcgctcagt gacaatatcg cccattttat
     1441 tggcgtaaaa gcggcggttt tgcacgtgga catagccgcg ctcttgaatg gtggaaataa
     1501 tcgaggcgta ggtggaaggg cggcctatgc cgcgtttttc taattctttc accaggcttg
     1561 cttcggtgta gcgggcggtg ggtttggtga agtgctgatt gccttgcacg gagtgcaggg
     1621 agagggcttg gccgactttc atatccggca acacgccttc atcgtctttt tttgctacgg
     1681 cgggcagtac ttttaaataa ccgtcgaatt tcactacgcg gcccttggcg cgcaattggt
     1741 agccctgggc ttcggcgatc acgctggtgc tggtaaattg ggccgggggc atttggcagg
     1801 caacaaattg ttgccaaatc aatgcgtaca ggcgttcggc atcgcgctcc atatctgtca
     1861 attggctggg cagacgagtg acttcagagg ggcgtatggc ttcgtgagcc tcttgggcgc
     1921 cttctttgct ggaatacacc acgggttttt ccggcaggta ctgcttgccg taggtgattt
     1981 cgatataggc gcggcagttc tccaccgcct cggcgcttaa attggtggag tcggtacgca
     2041 tgtaggtgat gtaacccgcc tcgtacaggc gctgggccat catcatggtt tttttcactc
     2101 caaagcccag gcgtgtgctg gcggcttgtt gtagcgtgga ggtaataaac ggcgccgaag
     2161 gtttggagga ggtgggctta tcctcccggg cgattaccgc aagggcgctt tgttttaccg
     2221 cctggacagc cttttgggct tcggcttggg ttttgggttc aaagcttgcg ccttggtacc
     2281 tcaccacact gaaattcacc cgctcctgtt tggcagtatc aagctcggcg gtaacatccc
     2341 aatactcttc cgggataaag gcgcgaattt ctttttcccg ctcgctgatg aggcgcacgg
     2401 ccaccgactg cacgcggcct gcggagagac cgcgggcgat tttctcccag agcagtggcg
     2461 agagcatgta gccgacgatg cgatcgagaa agcggcgcgc ctgttgggcg ttgacgcggt
     2521 tcatatccac ccggccaggg tttttaaagg cttcttgaat ggcggatttg gtaatttcgt
     2581 taaacaccac ccggcggtag cggctctcat ccccacctat ggcttcgcgc aggtgccagg
     2641 cgatggcctc cccctcgcgg tccaagtccg ttgccaggta gatttggtcg gcatcggcgg
     2701 cgagtttgcg cagttcttca atgaccttct ctttgcccgg tagaatttcg tagtgggcct
     2761 gccagttgtt atctgggtct atgcccatgc gtttgactaa ttgttcgcgg ccttttttgg
     2821 ctttgtagct gactttttct tctgccgaca ttttgcgggt ttgggcagct tgggcggcgc
     2881 gggctttagg gtctacggct tcgccggagc cgctggtggg taggtcgcga atgtggccga
     2941 tgctggattt cactacatat tggctgccga ggtatttgtt aatcgttttg gcttttgcag
     3001 gcgactctac gataaccagt gatttaccca tggtgtacgg ctcacttatg aatcagttgg
     3061 ggctgggggc aggctggaag ggggcgccgc ccggcggaag gtgcgcatat ataagtccta
     3121 ggttgcataa ggtcaaggat tagttcttgc ccagggtgat gcccctaacc tgtgaccaag
     3181 gcccattgca gtcagactag ttgaaaattt cccccttggt aggttaataa ttccgaacat
     3241 aaaagctgtg gttctgctgg cggctaaagt cgttttggcg cgctatacag gctatatata
     3301 agaagcaaga gtgcccgccg caagctatcc cttgttcaag tgccataaaa gcgtggcgaa
     3361 aaatgccaac cagcccagcg ctgcggccag acgccagaag ctgagagggt ctttttcc
//
LOCUS       DAOQGS010000458         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16130_length_3398_cov_3.702363, whole genome shotgun sequence.
ACCESSION   DAOQGS010000458 DAOQGS010000000
VERSION     DAOQGS010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16130_length_3398_cov_3.702363"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PKZ52_14635"
     CDS             complement(<1..686)
                     /locus_tag="PKZ52_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNG61058.1"
                     /translation="MSLFDDVSFGFCSKLPLLLQTEATECGLACLGMVAGFHGYRTDL
                     AALRGRFPVSLKGATLAQLMHIAQQMELATRALKLELEDLGQLRLPCVLHWNFNHFVV
                     LKQVSGKSVTIHDPAMGVRTLSLDEVSRCFTGVALELWPNPGFQPQQQKQPVRLTALL
                     GKVSGLYRSFAQVLLLALSLEVFALLSPFFLQWVIDNVIVSADRDLLTTLALGFGLLM
                     LMQEAVGLLRS"
     gene            complement(700..1953)
                     /locus_tag="PKZ52_14640"
     CDS             complement(700..1953)
                     /locus_tag="PKZ52_14640"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNG61059.1"
                     /translation="MSALFRPAALAAKQTSWLGEIVLIRPLSFTFLTAFAAVFAAIVV
                     LFLCWGGYTKRSTVTGQLVPDAGLVKVYTQQPGVVLEKRVLEGQRVQRGEVLYVLSSE
                     RQGNQGEVQAGISRQVQSRQHSLQDELGKTRLLQQEEREALAHKIDGLQAEAAKLDGQ
                     IDGQRSRVKLAEDTLARYQGLLAQDFIAREQVQQRQEELLDQRARLQGLERERIAAGR
                     ELAAQRNELSGLSLKHQNQLAAIERSIAGTSQELAESEAKRSLLVTAPEGGMVTAGVA
                     EVGQTVEPGKPLISVVPLGARMQAHLYAPSRAVGFVKPGDAVLLRYQAYPYQKFGHVR
                     GRVLQVARTALLSQELAGVAGSQPGGGEPVYRITVELAQQTINAYGKPQPLQAGMLLD
                     ADVLQDTRRLYEWVLEPLYSLTGKL"
     gene            complement(2099..2341)
                     /locus_tag="PKZ52_14645"
     CDS             complement(2099..2341)
                     /locus_tag="PKZ52_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61060.1"
                     /translation="MLKKLSNYIVAKEEECRVKAGISRFGLWLRNCLVFFFAELYVIF
                     GNDGAGFDAPVIIGALVVYVLVCWVLWIFVRENHKG"
     gene            complement(2595..3263)
                     /locus_tag="PKZ52_14650"
     CDS             complement(2595..3263)
                     /locus_tag="PKZ52_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61061.1"
                     /translation="MVAVALTAFFLPFVPLLVLDVNKVGGWVFVENGVSEDRARLLVY
                     GGEAASKRKWGWVPPVIYLFCNSNTCLSIYGIEQAKANSIQSGLFSLIVISGRGMSAE
                     IVAHELSHAVIYRKSGFMRRIMLPVWLEEGVATYLSEDKRFLGYSLIVGRDEAKRSAV
                     ELSSIPEREWDHISSVDPFYTYGRASGVAAYCTRRDQGFIAKLIDVVNDREKIFLLLD
                     QCLE"
BASE COUNT          931 a         1080 c          717 g          670 t
ORIGIN      
        1 gaacggagca agccgaccgc ctcctgcatc agcatcaaca atccgaatcc cagcgccagc
       61 gtggtcaaca aatcgcggtc tgccgacacg atcacattat cgatcaccca ttgcagaaaa
      121 aacggactga gcagagcaaa aacttccagc gacagcgcca gcagcaagac ctgggcaaac
      181 gaacgatata agccgctaac cttgcccagc aaagctgtca gccgaaccgg ctgcttctgc
      241 tgctgcggct ggaaccccgg attgggccac agctccagcg caacaccggt aaaacaacgc
      301 gacacttcat ccagcgacaa ggtgcgcacc cccatcgccg gatcgtgaat ggtcacgctc
      361 ttgccgctta cctgtttcag taccacgaaa tggttgaaat tccagtgcaa tacgcaaggc
      421 agccgcaact gacccaagtc ttccagctcc agcttgagcg cgcgggtggc cagctccatt
      481 tgctgggcaa tatgcatcaa ctgcgccagc gttgccccct tgagcgacac cggaaaccgg
      541 ccgcgcagag cggcaagatc ggtgcgatag ccatgaaaac cggccaccat gccaaggcag
      601 gcgaggccgc attcagttgc ctcggtttgc aataacagcg gcaatttgct gcaaaacccg
      661 aaggacacat catcaaacaa agacatcgta tttcttcaat caaagtttgc cggtcaggct
      721 atataaaggt tccagcaccc actcatacaa acggcgcgta tcctgcaata catcggcatc
      781 cagcaacatg ccggcctgca gcggctgcgg cttgccatag gcgttaatcg tctgctgcgc
      841 cagctcgaca gttatccggt ataccggctc gccaccgcca ggctggctgc cggccacgcc
      901 agccaattcc tgactgagca atgcggtgcg cgccacctgt aacacccggc cgcgcacatg
      961 gccaaatttc tggtacggat aggcctgata gcgcaacaac acagcgtcgc ccggcttgac
     1021 aaagcccacc gcccggctcg gcgcatacaa gtgcgcctgc atccgggcgc caagcggcac
     1081 gacactgatc aatggcttgc cgggctcgac ggtctgcccg acttcagcta ccccagccgt
     1141 caccatcccc ccttccgggg ccgtcaccag caggcttcgc ttggcctcgc tctcggcaag
     1201 ctcctggctg gtgccggcaa tgctgcgctc aattgcggcc aactggttct ggtgtttcag
     1261 cgacaagccg ctcaattcgt tgcgctgcgc cgccagctca cgtccggccg cgatccgttc
     1321 gcgctccagc ccctgcaggc gggcgcgttg gtccagcaat tcttcctgcc gctgctgcac
     1381 ttgttccctg gcgatgaaat cctgtgccag caagccctga taacgcgcca gcgtatcttc
     1441 cgccagtttc acccggctac gctgcccgtc gatctgccca tccagcttgg ctgcctcagc
     1501 ctgcaggccg tcgatcttgt gcgccagcgc ctcgcgctct tcctgctgca gcaatcgtgt
     1561 tttgccaagc tcatcctgca gactgtgctg acggctctgc acctgccggc tgatgcccgc
     1621 ctgcacctcg ccctgattgc cctgccgctc gctcgacagc acatacagca cctcgccacg
     1681 ctgaacacgc tgcccctcca gcacccgctt ctccaacacc acgcctggct gttgggtata
     1741 taccttcacc aagcccgcat ccggcaccag ttgcccggtg accgtgctgc gcttggtata
     1801 tcccccccag cagaggaaca aaacgacaat agccgcgaaa acagcggcaa aagcggtgag
     1861 aaaagtaaac gacaacggcc ggatcaggac tatctcaccg agccaactgg tttgcttcgc
     1921 cgccaaggcg gctgggcgga ataacgcact cacagcgcct ccggcaagaa aatcaaagca
     1981 tcgacaatct gtaacaaact cataatcata agcagcggtt tcttatcaag caggtatctg
     2041 gcaagcaata ttgatcaggc aaaacaaaat aagcagccaa tcaccaatcc acaggcaact
     2101 agcctttatg attttcacga acaaaaatcc acaaaaccca gcaaaccagc acatacacaa
     2161 caagtgcccc aattattaca ggcgcatcaa agccagctcc atcattgcca aaaatcacat
     2221 acaattcggc aaaaaagaat acaaggcaat tccgcaacca aaggccaaaa cggcttattc
     2281 ccgccttaac cctgcactcc tcttcttttg caacgatata gttacttagt tttttaagca
     2341 ttcacaaaat tgccagtagc agccagatta acaatcgcat cggctctgtt gcataaaatc
     2401 cagcaagtga ctgatttata aagaaattcg caaaatcaat tattttgcga atttctcaac
     2461 aaaatcaatg gcatggcgtg atttgtgcaa caacgccata taaacctacc gacaacagta
     2521 aaaatcatta ccaaacgcca agcggccaac cagacagcaa gctcaaacac cacatacaaa
     2581 taaagaaaaa atcatcattc caaacactga tctaacaata aaaatatttt ttctctgtca
     2641 tttacaacat caatcaactt agcgataaac ccctgatcac ggcgggtgca atacgctgca
     2701 acaccgctag cccgcccata tgtataaaac ggatcaacag aactgatatg atcccactct
     2761 ctctcaggga tagacgagag ttccacagcc gacctttttg cttcgtctct accaacaatt
     2821 aaagagtacc caagaaatct tttatcctca ctcaagtatg ttgccacccc ttcctccagc
     2881 caaacgggca acataattcg tctcataaaa ccagacttcc tgtatataac agcatgagac
     2941 aattcatgag caactatttc ggcagacatt cccctgccgg agataacaat caaagagaat
     3001 aggccgctct gtattgaatt agctttagcc tgctcaatcc cgtaaataga taggcatgta
     3061 ttagagttac aaaacagata aattacaggc ggcacccaac cccatttcct ttttgaggct
     3121 gcctcccctc cataaaccaa cagcctagcc ctgtcttccg acactccatt ctctacaaac
     3181 acccagccgc ccaccttatt aacatccagc acaagcaaag gaacaaatgg aagaaaaaaa
     3241 gcagttaacg cgacagcaac cacaacacag gctattagga gctttctcat ttgtaagcac
     3301 ttaccaaatc aaccggcctc accacaagca aggccggagc attagcttaa ccattaagcc
     3361 aacacggaag ctctacagat taatatgctc ggccactt
//
LOCUS       DAOQGS010000459         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16201_length_3387_cov_4.862545, whole genome shotgun sequence.
ACCESSION   DAOQGS010000459 DAOQGS010000000
VERSION     DAOQGS010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16201_length_3387_cov_4.862545"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            599..1966
                     /locus_tag="PKZ52_14655"
     CDS             599..1966
                     /locus_tag="PKZ52_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNG61062.1"
                     /translation="MRLNKLRIRPLVLGLCLAPALAQADTLKEIYQQALQNDHQFKAA
                     QANLAAGREAPVIGRAGLLPQVAGEAQLAKQELDVSGQPQISGRDATEKSYGVSLRQP
                     LFDLAVWNQYRASQVQGDLAEIQFLADQQDLIIRTSQAYFDALKAVDNANTAKAEETA
                     LARQLEQTKQRFAVGLIAITDVHEAQAAYDAAVAAKLLAQGQVGIAVQAVEVLTGKAY
                     TGLSPLQGKFPVTAPVPEARQEWVDFALKNNLQLKGAAGRAQVAEYNYKAKAAGHLPT
                     VYGSLSYEKADSSGDGYSGPAAYDLDSDSTTKVAAISLKVPIFTGGYTSASRRQALQQ
                     RYAADEGKLQAERDIVQATRSFHLSVMTGVASVKARQQSIVSSQSALDATKAGYDVGT
                     RDLVDLLRAQQNLFRAQRDYADALYSYVLGSLKLKQAAGMLSEKDVEALDNWLDKNAL
                     VKFDM"
     gene            complement(2328..>3387)
                     /locus_tag="PKZ52_14660"
     CDS             complement(2328..>3387)
                     /locus_tag="PKZ52_14660"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNG61063.1"
                     /translation="TGSSSSSSSSSSSSSSSSSSSSSSGKTSSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSGLGPIIARTTCPTAPPTRANNVVVPDYTQLTVPKSVYTPMMVE
                     QPIFKASRIDLPWNLYKPDQAAMNPGQKFPMVLSLHGGYGREVAQGNILVDGAPFLLG
                     ASTGLLTPENRAKYPAYIVMPWCRLQADCSFYSNEWASNGGANFQVASQPSKYGSAVI
                     ELIDYMINTYQVDPARIYVTGASMGGGGTWELAQRRPDLIAAAFPMAGHTPAQSFLDS
                     FAKSKVPVWAFSSSTDNNNPSRDTDAAVSYIKSKGGCAWVTKFDNIEHGHLLWQRSFL
                     EPGLWDWVFAQKQPRAGQ"
BASE COUNT          748 a          845 c         1008 g          786 t
ORIGIN      
        1 tagcagcttg ttgaaatact accttgctag cactggcggc gttaaatttg gcgaaaaaat
       61 cctcatttac tgcgtgtaaa ctccgttttt ttcgccaaat ttgccttgcc agagcgtcgc
      121 cgcctacgta tttcaacaag ctgctagatg cgggcataca tttaatttag atgcgcaggc
      181 cccaatcaac agcgctcagg ccgtagcaca ggcgctggtg ttttccaaac actcgtgccg
      241 ccctattgca ggcagagcat ttgcaattgc ccgttattgg actggggttt tgccattaaa
      301 cgtaaaagag tttcactggg cggaaacttt ccgcgcggat acttacctta tgcggcgtcg
      361 ccgcgccgct gcactgcctt gccctgcaat agccgcgttg gaatagctag gttgcacacc
      421 gacccggtgc ctcggggctg atgtgtttta gtggagggct aggcttggtg tagcggcggg
      481 taaaattggc gcttaagcgc tacgccagcg ggtgtttttt tacaaaaaga tgttaggcat
      541 tggcggtatg agtggttaca ctgccgcagc tgctttaatt gaaccgatta ggatcactat
      601 gagattaaat aaattgcgca tacggcccct agtgttgggt ttgtgtttgg cccctgcctt
      661 ggctcaggcg gataccctaa aagaaattta ccaacaagcg ttgcagaatg atcaccaatt
      721 taaggccgct caggccaact tggcagctgg gcgcgaggcg ccggtcattg ggcgcgcagg
      781 gctgctgccc caggtggccg gtgaggcgca gttggccaaa caggaattgg atgtaagcgg
      841 ccaaccgcaa atcagcggtc gcgacgccac cgaaaaaagt tatggtgtca gcctgcgcca
      901 accgctattt gatcttgcgg tgtggaatca ataccgcgcc agccaagtgc agggggatct
      961 ggcggagatt caattcttag ctgaccagca ggatttaatt attcgcacct cgcaggcgta
     1021 ttttgatgct ttaaaagcgg tggataacgc caataccgcc aaggccgaag agaccgccct
     1081 ggcccgccag ctggaacaga ccaaacagcg ttttgcggtg ggcctaattg ctattacaga
     1141 tgtacacgag gctcaagccg cctacgacgc cgccgtggcg gcgaagttat tagcccaggg
     1201 ccaggtgggc attgcggtgc aggcggtgga agtattgacc ggcaaggctt ataccggcct
     1261 tagccctctg caagggaaat tccctgttac cgcccccgtg cctgaggcgc gccaggagtg
     1321 ggtggacttt gccttaaaaa ataacctgca attaaaaggc gccgctgggc gcgcccaggt
     1381 ggctgaatac aactataaag ccaaggcagc gggccatttg cccactgtgt atggcagcct
     1441 gagttacgaa aaagcggatt ccagcgggga tggctactcc ggccctgcag cctatgattt
     1501 ggattcggat tccaccacca aggtggcggc tattagcctt aaagtgccta tttttaccgg
     1561 tggctacacc tcggccagcc gccgccaagc cctgcagcag cgctatgccg ccgatgaggg
     1621 caagctgcag gcggagcgcg atatagtgca ggctacccgc tcgtttcacc tttcggtgat
     1681 gaccggtgtg gccagtgtta aagctcgcca gcagtctatt gtgtcttcgc aaagtgcttt
     1741 agatgccacc aaggcgggct acgatgtagg cacccgggac ctggtggatt tattgcgcgc
     1801 tcagcagaat ttattccgcg ctcagcggga ttatgccgat gccctttact cctatgtgct
     1861 ggggagtttg aagcttaagc aggcggcggg catgttgtcc gagaaggatg tggaggcgct
     1921 ggataactgg ctggataaaa atgccttggt gaaatttgat atgtaaataa tccgggcgag
     1981 caaaaccctg atacaaaaaa accggccttg gccggttttt ttgcgtctat ataaaaataa
     2041 aaggcgcttt ccggaattca cttccactgt aggtgttttt gcgcaggtca atttacccat
     2101 gcgggacagc cggagtatgg caatgtcatt tggttgagcg ttttgcaccg atacttactg
     2161 gatcatgcgc acagcccaaa acccctcccc attttaagta ggagacaggg aggggcagaa
     2221 taaataaccc acaggggatg aaaagctaat cgtgaattac aggaagtagc cataaaaaaa
     2281 ccccttccag ccggtgctgg aaggggtttt gttgatgggc ttaaggctta ttgaccggcg
     2341 cggggttgtt tttgcgcgaa tacccagtcc cacaggcctg gctctaagaa cgagcgctgc
     2401 cataacagat ggccgtgttc gatattgtcg aacttggtta cccaggcaca gccgcctttg
     2461 ctcttgatgt agctgaccgc agcatcagta tcgcgcgagg ggttgttgtt atcggtgctg
     2521 gagctaaatg cccatacggg tactttgctc ttggcaaacg aatccaagaa ggactgtgcg
     2581 ggggtgtggc cggccatggg gaaggctgcg gcgattaggt ctggacggcg ctgcgccaat
     2641 tcccaagtgc cgccaccacc catggaggcg cccgtgacgt aaatacgggc agggtctact
     2701 tgataggtat tgatcatgta atcaatcagc tcgatcaccg ctgagccgta tttagacggt
     2761 tggcttgcca cctggaagtt ggcgccaccg ttggaggccc actcgttgga gtagaacgag
     2821 cagtcggcct gcagacggca ccagggcatc acaatatagg ccgggtattt ggcgcggttt
     2881 tctggtgtta acaggccggt gctggcgcct aacaagaagg gcgcgccgtc caccaaaata
     2941 ttgccttggg ccacttcacg gccgtagccg ccgtgcagag acaataccat cgggaacttc
     3001 tgccctgggt tcatggccgc ttgatcgggt ttgtagaggt tccagggtag gtcgatgcga
     3061 ctggctttga agatgggttg ctccaccatc ataggtgtgt acacgctctt aggcaccgtc
     3121 agctgggtat agtcgggcac taccacgtta ttggcgcgag taggtggcgc agtggggcag
     3181 gtggtgcggg cgatgatggg gcctaggcct gagctgctgc tggagcttga actactgctt
     3241 gagctgctgg aagaagagct agagctgctg ctggaggacg aacttgagct gctagatgag
     3301 ctggaggttt tgccgcttga gcttgaactg ctagagcttg agctgctgga acttgaacta
     3361 ctgcttgagc tgctgctgga accggtt
//
LOCUS       DAOQGS010000460         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16366_length_3364_cov_4.812632, whole genome shotgun sequence.
ACCESSION   DAOQGS010000460 DAOQGS010000000
VERSION     DAOQGS010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16366_length_3364_cov_4.812632"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKZ52_14665"
     CDS             <1..397
                     /locus_tag="PKZ52_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61064.1"
                     /translation="GIWQIKLNAKAQTAKNQLEISLAPAILVADASDETSQNFRAQIA
                     ALIERFHGKTLSPDSEEVVRYTNLFLQLRQNKIDRKSGTSLTETNVACDYNSNGVAWN
                     QWAADPTSSLSAWRTLVAALMADYNYIYE"
     gene            406..1686
                     /locus_tag="PKZ52_14670"
     CDS             406..1686
                     /locus_tag="PKZ52_14670"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNG61065.1"
                     /translation="MKRRTLLGYLGLTGLGIALPSVPCRVWAASSAYKGLLYINLQAS
                     GGWDVTSFCDPKINQPNEKIINNWATTAEVQKAGNIAYAPVGNNARFFKQHFDKMLVI
                     NGINSRTNAHVAGRLFNHTGSQKNGTPQLSALHAFAHNQGLAMPLMAYGDAVTANLVA
                     PTKLSRDIFQIVEPNIRTPGQATQWLANEDLAAIKAIQAERANSLKDGKLQLAKQQQQ
                     VNEYYDSVFADTEGFKNFSALYQSLNEGEYVKDSYTDAIKFALTAFTTGLGVSADITI
                     GGFDTHDEHDVRGVKALDDLTNAASMLWHYAEQLGIGDRLVVSIASDFGRTPYYNDGR
                     GKDHWTYGSTILMQKNVPWTNRVIGKTTELHKALKINPQTLQVDANGIELEPSHVHQN
                     LRNYLGIAANPAAVRFKLDAGTQVEFLNSTAQTA"
     gene            1931..2914
                     /locus_tag="PKZ52_14675"
     CDS             1931..2914
                     /locus_tag="PKZ52_14675"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNG61066.1"
                     /translation="MKTPVRVTVTGAAGQISYSLLFRIAAGDMLGKDQPVILQLLEIT
                     PALNALKGVAMELDDCAFPLLAGIVTTDDPNVAFKDTDYALLVGARPRGPGMERKDLL
                     EANAAIFSVQGKAINDHASRQVKVLVVGNPANTNALIAQRNAPDIDPRQFTAMTRLDH
                     NRALSQLGEKLAVSINDINKITIWGNHSSTQYPDLFHAVVKGEVAINKVEREWYESSY
                     IPTVQQRGAAIIAARGASSAASAANAAICHIRDWALGTADGDWVSMGVYSDGSYGIEE
                     GLIYSFPCTCSNGQWSIVQGLDIDAFSRARMSATEKELQEERDAVKSLLPA"
     gene            2984..>3364
                     /gene="bcp"
                     /locus_tag="PKZ52_14680"
     CDS             2984..>3364
                     /gene="bcp"
                     /locus_tag="PKZ52_14680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNG61067.1"
                     /translation="MSLVQVGAPAPAFTLQNQEGSSISLDSLRGQWLAVYFYPKASTP
                     GCTVQACGLRDTMAELRSLGVQVLGISPDAPPKLKKFAQAQSLPFTLLSDVDHAVAEA
                     YGVWALKKFMGKTFMGVVRTTFIID"
BASE COUNT          876 a          902 c          793 g          793 t
ORIGIN      
        1 gggtatttgg cagataaaac tcaacgccaa agcacaaacg gccaagaatc aattggaaat
       61 cagccttgca ccagcgattc tagtcgccga tgccagcgat gagaccagcc aaaatttccg
      121 cgcacaaata gcggcattaa ttgagcgctt tcacggcaaa acactatcgc ccgattctga
      181 agaagtggtg cgttatacca atttatttct acaactgcgg caaaataaaa tcgatcgcaa
      241 aagcggcact tctctgaccg aaacaaacgt agcctgtgac tataattcca acggtgttgc
      301 atggaatcag tgggccgctg acccgacctc aagcctcagt gcatggcgca ccctagtcgc
      361 cgcattaatg gctgattaca actatattta cgagtaagaa atgttatgaa acgccgcacg
      421 ctacttggct atttaggttt gacaggtttg ggcattgccc taccctcggt gccctgccga
      481 gtgtgggctg ccagcagcgc ctataaaggc ctgttatata ttaatttgca ggccagcggc
      541 ggctgggacg taaccagttt ttgcgacccg aaaattaacc agcccaatga aaaaattatc
      601 aataattggg cgacaacggc tgaagttcaa aaggcgggca atattgccta tgccccggtt
      661 ggcaataatg cgcgtttttt taagcaacac ttcgacaaaa tgctggtgat taacggtatc
      721 aatagccgca ccaatgctca tgttgcaggc cgcttattca accacacagg ttcccagaaa
      781 aacggcacac cacaactctc tgccctgcat gcttttgcgc acaatcaggg gctggcgatg
      841 cccttaatgg cgtatggcga cgcagtcaca gccaatcttg ttgcacccac aaaattatcc
      901 cgtgatattt ttcaaattgt tgaacccaat atccgcacgc ctggacaagc cactcagtgg
      961 cttgccaatg aagatctggc agccatcaaa gccatacaag ctgagcgcgc caattcgtta
     1021 aaagatggca aattgcaact ggccaaacag caacaacagg taaatgaata ttacgactcc
     1081 gtgtttgccg ataccgaggg ctttaagaat ttttctgcgc tttaccaaag cttgaacgag
     1141 ggcgaatatg ttaaggatag ttataccgac gccataaaat ttgccttaac ggcctttacc
     1201 acgggcttag gtgtaagcgc tgatatcacc ataggtggct ttgacaccca cgacgaacac
     1261 gatgtacgcg gcgtcaaagc actggacgac ctgacaaacg cagcctccat gctctggcat
     1321 tacgcagaac aattgggcat tggcgatcgc ctcgttgtaa gtatcgccag cgattttggc
     1381 cgcactccct attacaacga tggcaggggt aaggatcact ggacctatgg cagcactatt
     1441 cttatgcaga aaaatgtacc ctggaccaac cgtgttatcg gcaaaaccac cgagctacac
     1501 aaagccttaa aaataaatcc gcaaaccttg caggttgatg ccaacggcat cgaattagaa
     1561 cccagccatg ttcatcaaaa tttgcgcaac tatttaggta tcgctgccaa cccagcggca
     1621 gtgcgtttta agcttgatgc cggcactcaa gtagagtttt taaattcaac cgcccagaca
     1681 gcttaaccgc ctgttgagaa gcttgagatt gcctttggta tacactgaaa gtctttccct
     1741 tttgggtggc cagtagattt ttgcggaatt tttctggcca attcatcggc aaggttaaat
     1801 gcgcacaaac cgtaaatgac gccccatttc atattgcggg gtgactttta ggctcacttc
     1861 tttataatgt cgccccgcta tcctgaagcc atcaggacca ccatttattt gctataagcg
     1921 gagagtcaga gtgaaaacac ccgtacgagt taccgtcacc ggcgcggctg gtcaaattag
     1981 ttactcccta ttattccgca tcgccgctgg cgacatgttg ggtaaagatc aacccgttat
     2041 tctgcaatta ttagaaatca cccccgcatt gaacgccctt aaaggcgttg ccatggaact
     2101 tgatgactgc gccttccccc ttttggccgg catagtgacc accgacgacc ccaatgttgc
     2161 ttttaaagat actgattatg cccttcttgt aggtgcccgc ccccgcggcc caggcatgga
     2221 gcgcaaggat ttactcgaag ccaacgccgc tattttctcg gtacaaggca aggcgattaa
     2281 cgaccacgcc tcgcgtcagg tcaaagtgct ggtagtgggt aaccctgcca ataccaatgc
     2341 actaattgcc cagcgcaatg cgccggatat tgatccgcgc cagttcaccg ccatgacgcg
     2401 cttagatcac aaccgagccc tctcccaatt aggtgaaaaa ctcgctgttt ccatcaacga
     2461 catcaacaaa atcaccatct ggggcaatca ctcctctact cagtaccctg atttattcca
     2521 tgctgtggtt aagggtgaag ttgctatcaa taaggttgag cgggaatggt acgaatccag
     2581 ctacatccca actgtacaac agcggggcgc cgcgatcatt gccgctcggg gggcatccag
     2641 cgcagcttcc gctgccaacg ccgccatctg ccatattcgc gactgggcgc tgggtacagc
     2701 ggacggcgat tgggtgagca tgggcgtata cagtgacggc agctacggca tcgaagaagg
     2761 cctgatttat tccttcccct gcacctgctc taacggtcaa tggagcattg tgcaaggcct
     2821 tgatatcgac gctttctcac gggcgcgcat gtcagcaacc gaaaaggaac tgcaagaaga
     2881 gcgcgatgct gttaaatccc ttctgccagc ataagctggt ggcctcctgc gtttaaaaac
     2941 aaaaagcacc ttagcctggg tgcttttttt ttgaggaatc attatgagcc ttgtccaagt
     3001 cggtgcccca gcacctgcgt ttacgcttca aaaccaagag ggcagttcaa tttcactgga
     3061 ttcattgcgc ggccaatggc tggcagtgta tttctatccc aaggcctcca cacccggttg
     3121 caccgtccag gcctgcggac tgcgcgacac catggcggaa ctgcgcagcc tcggcgtaca
     3181 ggtcctaggc attagcccgg atgcgccacc aaagttgaaa aagtttgcac aggctcagag
     3241 cctgccgttc accctgttga gcgatgtcga tcacgctgtt gccgaagcct acggtgtttg
     3301 ggcgttaaaa aaattcatgg gaaaaacctt tatgggtgtt gtgcgcacca catttattat
     3361 cgat
//
LOCUS       DAOQGS010000461         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16461_length_3352_cov_4.553837, whole genome shotgun sequence.
ACCESSION   DAOQGS010000461 DAOQGS010000000
VERSION     DAOQGS010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16461_length_3352_cov_4.553837"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PKZ52_14685"
     CDS             complement(<1..1283)
                     /locus_tag="PKZ52_14685"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNG61068.1"
                     /translation="MHNGADILFEHTYAGLGEAFATPCAPTPVAAPRLIAFNWDLARQ
                     LNITLPDDPLLLARHFSGNQLFPSAHPCAQKYAGHQFGSFNPRLGDGRALLLGEVIDE
                     NGARKDVQLKGAGPTPYSRNGDGRAALGPVLREYLISEAMYHLGVSTTRALAAVSSGE
                     AVWRQGQLPGAVLTRVASSHIRVGTFEFFYRQGQLEEVKTLADYVIARHYPQAQTAAN
                     PYLALLDGVITAQAELVAHWMQLGFIHGVMNTDNTSISGETIDYGPCAFMDKFVFHQC
                     FSSIDMEGRYAYSRQPQIALWNLSRLAECLLALLDDNQQNAIAKAEASLERFHATYQN
                     AWLRGWRKKLALDMQSDDTDARFILDYLQCLRIYGLDFSATNRNLAAAITEALPNTQL
                     SAKPQLSAWLAQWRERILARGLSTQELKAELLAANP"
     gene            complement(1516..1788)
                     /locus_tag="PKZ52_14690"
     CDS             complement(1516..1788)
                     /locus_tag="PKZ52_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNG61069.1"
                     /translation="MAAIHSELLNLKNLGMASVNILRAVGINNYQDLSEIGAVEAYCR
                     IKARGIHVSKVMLYALQGALLDMHWNDLPPALKAQLVEEAEALEQV"
     gene            1982..2641
                     /locus_tag="PKZ52_14695"
     CDS             1982..2641
                     /locus_tag="PKZ52_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNG61070.1"
                     /translation="MFEQALGLDDSHINYGLAERPIHQNSANALARLRAAAQHAGFEL
                     RLASGFRSFSQQLTIWNAKARGERRVLDSNEQPIDIVPLTDAEKMFAILRWSALPGTS
                     RHHFGTDWDVYAENLLPPNYRLQLTQEEYLHGVQKEFNRWLTEFIRTDGEFVRPFEAG
                     SSAVAAEPWHISFLPEARKLTPWVKQETLLNTLSQADIELKTQIQQQLPKILAEYVRP
                     V"
     gene            2672..3316
                     /locus_tag="PKZ52_14700"
     CDS             2672..3316
                     /locus_tag="PKZ52_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNG61071.1"
                     /translation="MLVIFDCDGVLVESEALAAQAFSQVLARYEIVLSADECERLFIG
                     KTLTQCIETLQQSYPGRLPGDFREQLDEASEALFAQQLAPVQGVEAVLTAVRARGIAL
                     AVASNGGLAKIQLNLTRTGLIDYFAANIVSAEHVPQPKPAPDVYLAAAEAQGVPAQFC
                     CVVEDSNVGALAGLSAGMRVLLFRPPWRTHHSPAPAGVEVFEDMQQLLGLILRY"
BASE COUNT          737 a          851 c          944 g          820 t
ORIGIN      
        1 gggttggccg ccaatagttc tgcttttaat tcctgggtac ttaaacctcg cgccagtatt
       61 cgctcgcgcc actgggcgag ccaagcactt aattggggtt tagcgctaag ctgggtgtta
      121 ggtaatgctt ccgttatcgc ggccgccaaa ttacggttgg tagctgaaaa atccagaccg
      181 tatatgcgca aacactgcaa ataatccaag ataaaacgcg catctgtgtc atcgctttgc
      241 atatcgaggg cgagtttttt gcgccagccg cgcagccagg cgttttgata agtagcatga
      301 aaacgctcaa ggctcgcctc cgctttagct atggcatttt gttggttgtc atccagcagc
      361 gctaacaaac actctgccaa acggcttaaa ttccacaggg caatttgcgg ttggcggctg
      421 taggcatagc ggccttccat atcgatggag ctaaaacatt ggtgaaaaac aaatttatcc
      481 ataaacgcgc aggggccgta atcgatggtt tcgccgctga tactggtgtt gtcggtattc
      541 atcacaccgt ggataaaacc caattgcatc caatgcgcaa ctaattctgc ctgcgcggtg
      601 atcaccccat ctaacagggc gagataggga ttggcagcgg tttgcgcctg cgggtagtgg
      661 cgggcaataa cgtaatcggc gagggttttt acctcctcta gttgcccttg gcggtaaaaa
      721 aattcaaatg tgcccacccg tatgtggctg ctggccaccc gggtgagaac ggcgcccggc
      781 aattggccct ggcgccacac ggcttccccg ctgctgacgg ctgctaaggc gcgggtggtg
      841 gacacgccca aatggtacat ggcctcgcta attaaatact cccgcagtac aggcccgagg
      901 gcggcgcggc catcaccatt gcgtgaatag ggggtggggc cggcgccttt taattgcaca
      961 tctttgcgcg cgccattttc atcgatcacc tcgcccaata ataaggctcg gccatccccc
     1021 aggcgcgggt taaaactgcc gaattggtgg cctgcgtatt tttgtgcaca ggggtgggcg
     1081 ctggggaaga gttgattacc gctaaagtgg cgcgccaaaa gcaatggatc atcaggtagg
     1141 gttatattca gttggcgcgc taaatcccag ttaaatgcaa ttagtctggg ggctgccacc
     1201 ggtgtggggg cgcagggcgt tgcaaaggct tcgccgagac ctgcgtaggt gtgttcaaag
     1261 agaatatctg ccccgttgtg catagctacc tcactcatgg ctgctttaac tagtggcgga
     1321 actagtgttt agccgcgcaa aaagctgcat tgtgttctcg cctaggggct aacacaagcg
     1381 ttcacagggg gctatgaggg ataaaaatgg tggcaaaaag cagctgaatt tatgcggggc
     1441 caatgccaat acataacacg aactggctgc gggcctgaac gcgcagccag tacatgagcg
     1501 ggttacctta aggccttata cctgctccaa agcctcggct tcttccacta gctgagcctt
     1561 taatgccggg ggtaggtcat tccagtgcat gtctaagagc gcgccttgca gcgcatagag
     1621 catgacttta gaaacgtgga ttccccgcgc cttaatgcgg caataggctt ctaccgcgcc
     1681 tatttcggat aaatcctggt aattattaat gcccacagcg cgtaagatgt taacggacgc
     1741 catgccgagg ttttttaaat tgagaagctc actatgaatt gctgccatgt tgttacttcc
     1801 tgcacgtttt attatcttgg gcggtatcgt ctgtgcgatt tgcctgggag tttacagttg
     1861 cattaacctc atgccctagc agcatagacc gcatttggtt gcggtgcaaa attcaaccat
     1921 cattttttga tctacgcacg gcacaggcag tagcctttgc gatcttgcag agagtaaccc
     1981 tatgtttgaa caggccctgg ggcttgacga cagtcatatc aattacggtt tagctgaacg
     2041 ccccatccac caaaatagtg ccaatgcctt agcgcgcctg cgggctgcgg ctcaacatgc
     2101 gggttttgaa ttgcgcctgg ccagcggctt tcgcagtttt agccaacaat taaccatctg
     2161 gaacgccaag gcccgcggtg agcgccgggt gttggattct aatgagcagc ccatcgatat
     2221 agtgcccctc accgatgcgg aaaaaatgtt tgccattttg cgttggtcgg cactgcctgg
     2281 cacctcgcgc catcatttcg gcaccgattg ggatgtgtat gccgaaaatc tgctgccgcc
     2341 caattaccgt ttgcagttga cccaagagga atacctgcac ggcgtgcaaa aagaatttaa
     2401 tcgctggtta acggaattta tccgcacaga tggtgaattt gtgcggccgt ttgaagcggg
     2461 ctcatccgct gttgccgccg agccctggca tattagtttt ctgcccgagg cgcgcaagct
     2521 caccccctgg gttaaacagg aaaccctgtt aaataccctc agccaagccg acatcgagct
     2581 taaaacacag attcagcagc agctgccaaa aattttggcc gaatatgtac gccctgttta
     2641 agccgggtag tgatgtggac ttggggcgct tatgttggtg atttttgatt gtgacggcgt
     2701 gttggtggag agcgaggccc tggcggcaca ggctttttcg caggtgctgg cgcgttatga
     2761 gattgtgttg agtgcagatg agtgcgaaag gctgtttatc ggcaaaaccc tcactcagtg
     2821 catagagacc ttgcagcaga gttaccccgg gcgcctgccc ggggattttc gcgagcagct
     2881 ggatgaggcc agtgaagcct tatttgccca gcagctagcc cctgtgcagg gggttgaggc
     2941 ggtgttaaca gctgtccgcg cgcgggggat tgctttggcc gtggcgagta acggcgggct
     3001 tgccaaaatt cagctgaacc tcacccgcac cgggttaatt gattattttg ccgctaatat
     3061 tgtcagcgcc gaacatgtac cccaacccaa acctgcgcca gatgtttatt tggcggccgc
     3121 cgaggctcag ggcgtaccgg cgcaattttg ctgcgttgtg gaggattcca atgtgggggc
     3181 gctggcgggt ttgagtgcgg gtatgcgcgt gctgttattt aggccgccct ggcgcaccca
     3241 tcacagtccg gccccggcgg gggtggaggt attcgaagat atgcagcagt tattagggct
     3301 gattttgcgt tattaatttc tcccaggctt ttagttaaaa accgcagtta ac
//
LOCUS       DAOQGS010000462         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16692_length_3321_cov_5.998163, whole genome shotgun sequence.
ACCESSION   DAOQGS010000462 DAOQGS010000000
VERSION     DAOQGS010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16692_length_3321_cov_5.998163"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKZ52_14705"
     CDS             complement(<1..714)
                     /locus_tag="PKZ52_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNG61072.1"
                     /translation="MNIKNILQTGKIALGALALGLSLGAQADTSATGNNNGFYYSFWK
                     DNGNASWQLLSGGRYTLTWDRSTNNVVGGKGWNPGTNNRVISYTGTYNGSSSQNTYLA
                     LYGWTRSPLVEYYVIESWGSYNPSSCSGGTSYGSYQSDGATYDMRKCTRTNQPSIDGT
                     QTFSQYFSVRNPKKGFGQVSGTITFANHANAWKSKGLNLGNHFYQIMATEGYQSQGSS
                     DITVSEGPGSNSSSSSSSSS"
     gene            complement(2138..>3321)
                     /locus_tag="PKZ52_14710"
     CDS             complement(2138..>3321)
                     /locus_tag="PKZ52_14710"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HNG61073.1"
                     /translation="GGGGSGDGQKGSVTIGGVRYNLTQCTRTNQPSISGTSTFKQFFS
                     VRQDPLPWGAVQGSIDVAAHFKAWADAGMKLGTDFDYMVLATEGYDGNRQSAGNAELW
                     ISTGTATSSSSSSSSSGGNSCGTVGGVPVCCDISADPDGDGMGMENNKVCTVTDKTVG
                     YTPPNPADVLAAINVGGTGAAVQVGNIYFAPNRYMTGGTANSTTATITGGNDLFKTEL
                     YGDFSASIPASNGRYSVELGFVEMYHTSTNARSFNVAIEGQRVLTNVDIFAEVGANTA
                     LKKTFEASVTDGKLDIALTTNTDNATLSSILVRKSTSSSSSSSSSSSSSSSSSSSSSS
                     SSSSSSSSNSSSSSSSSSSSSSSSSSSSGGQVAGALDTSALVLMLAGLIGFKRRSRAA
                     R"
BASE COUNT          881 a          778 c          778 g          884 t
ORIGIN      
        1 gctgcttgac gagctagacg atgaggaatt ggagcctggg ccttcgctta ccgtaatgtc
       61 cgaactgcct tggctttggt aaccctcagt agccatgatt tgatagaagt ggttacccag
      121 gttcaagcct ttacttttcc aagcgttagc gtggttggca aaagtgatgg tgccgctaac
      181 ttggccaaag ccttttttag ggttgcgaac gctgaagtac tggctaaagg tttgagtacc
      241 gtcgatagag ggctggttgg tgcgcgtaca tttacgcata tcataggtag cgccatcgct
      301 ttggtaactg ccgtagcttg taccaccaga acagcttgat gggttgtaag aaccccagct
      361 ttcgattacg tagtattcaa ccagcgggct gcgggtccaa ccataaagtg ccaaataagt
      421 gttttgcgag cttgagccgt tgtacgtacc agtgtaacta ataacacggt tgttggtacc
      481 tgggttccag cccttaccac ctaccacgtt gttcgttgat ctatcccaag tgagggtata
      541 gcggccgcca gacagtaatt gccagctagc attgccgtta tctttccaaa acgaatagta
      601 gaaaccatta ttgttaccag tggcgctggt atcagcctgc gcgcctaaag acaaacccag
      661 tgctaatgca cctaatgcta ttttgcccgt ttgcagaata tttttaatgt tcatagtaat
      721 ctccggcctt tataaatata ggaattaacg ttttggaggg cagcaaagaa gttgtttttt
      781 gaaatatttt ctcgctgctt aaaattattg ttctccactg ctcaaacggt ttttcacctc
      841 agtcgtgatc tagccatgaa agcaaaacaa cctcgcctat tatggtctaa attactacat
      901 gaagagttag cccttaccta tttgattcct tatcagctta acgcacgggg ctttacagct
      961 gctgtgaatt ggttccggta tgactattta atttatgtaa tacgtctcta attagaatta
     1021 aaaaatttcc tgcgatacgt taaaaatgac tattactcag cttcacggta tttgttggct
     1081 tttaccaaca cattaagatt atttggcgtc gtgtatttaa gccacaagct attagctgca
     1141 atactcagcc tgccggttga agtttcttta taaccaaccg ctggccaatg agacacatta
     1201 ctgtcaacca gctcgctgag ctaacacatc atctttttaa tttatggaat aaaaagcgtg
     1261 agggtttaac ctgcgtaagt ttccccatta gatcgttggc gttttatcag cggctgttta
     1321 cccagaacac acatctaatt aacctgaatc aatttacttt ttatccgctc gttttaacac
     1381 ctaggccgcg actctttggc aaggtagcgt ttcaacaggc tgttagttga tcaagccaat
     1441 ttagttagga acacagcctg actcaacctg agctctgagc aaaatgcgca tcagaaagcg
     1501 cttgagaaag tgtcacttaa tcaacacaca tcatgtttgg gcgatttaaa atagagttac
     1561 tcccgtcaac acaatacaca agtgccaaaa accgatcggg tagtaaattg tttttctcga
     1621 atacatatcg ccaatgctaa gtaaaaccaa gcctagcaac attatttggc tggcctgcaa
     1681 tcagcgcaaa gcgaataaaa agccaaaaaa acagcctaat tttcaacgca ggaaaaattt
     1741 cctgcaaaaa atttttttgt ccagctaata ggcgttcgaa ttgacagcgc tttttataca
     1801 aatcgcgctc atatacccac aggtggatac aattgttttt atgggggagc tgccaggtta
     1861 aaacactatt aacccggcgg caaaatagat cgtcctgatc tattttgccc tcgctccgct
     1921 gacacttgcg aaaatgcgtg gattttcttg ttttagcgga gctcgcggcg gcttgcgcct
     1981 ttggcgcggc atccttgccg cagttattaa cccggcataa aaagccgccg ggttaatatt
     2041 aatgattaaa gaaacatacg agcgacactt aggaaaatac agaatccata ggcaaaaaaa
     2101 agccgcctat aaaggcggct ttttatctgc cagtgaatta gcgagcggcg cgtgaacgac
     2161 gcttgaagcc aatcaaacca gcgagcatca atacaagcgc tgaggtatcc agcgcccctg
     2221 ccacctgacc accggagctg ctggaggaag atgatgagga gcttgagcta cttgacgaag
     2281 agctggagct attagacgaa gagctggagc tactcgatga agagctagag cttgagctgc
     2341 tcgatgaaga gcttgatgag ctagagctgg acgaggagct gctggtggat ttacgtacca
     2401 aaatggaact tagggtggcg ttatccgtgt tagtggtaag cgcaatatcc aattttccat
     2461 cggtaacact agcttcaaat gtctttttca atgcagtgtt ggcaccaacc tctgcaaaaa
     2521 tgtccacatt ggtcagcacg cgctggcctt caatcgccac gttgaatgaa cgagcattgg
     2581 tgcttgtgtg gtacatctcg acgaagccta actcaaccga gtagcgcccg ttgctagcag
     2641 gaatagaagc cgaaaaatca ccgtagagtt cagttttgaa caaatcatta ccgccggtga
     2701 tagttgctgt ggtggaattt gctgtaccgc cggtcatgta gcggttaggt gcaaagtaaa
     2761 tattgccaac ttgcaccgca gcgccagtac cacctacgtt aatagcggcc agaacatcag
     2821 ctgggtttgg cggggtgtag ccgactgttt tatcggtgac ggtacacacc ttgttatttt
     2881 ccatacccat gccatcgcca tctggatcgg cgctgatgtc acaacaaact ggtacaccac
     2941 ccacggtgcc acagctgttg ccgccagaag aactgcttga actggatgag cttgttgcgg
     3001 taccggtact gatccacagt tcggcattac cagctgactg cctgttgcca tcgtaacctt
     3061 cggtggcgag taccatatag tcgaagtcgg tacctagttt catgccagca tcagcccagg
     3121 ctttgaaatg ggcagccacg tcgatggagc cctgcacagc accccagggc agagggtctt
     3181 ggcgtacgct gaagaactgt ttaaaggtgg aagttccaga aatagacggc tggttggttc
     3241 gtgtgcactg cgtcaggttg taacgcacgc cgccgatggt gacggagcct ttttggccat
     3301 ccccactgcc accaccgcca g
//
LOCUS       DAOQGS010000463         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16696_length_3321_cov_4.075015, whole genome shotgun sequence.
ACCESSION   DAOQGS010000463 DAOQGS010000000
VERSION     DAOQGS010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16696_length_3321_cov_4.075015"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..668
                     /gene="can"
                     /locus_tag="PKZ52_14715"
     CDS             33..668
                     /gene="can"
                     /locus_tag="PKZ52_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNG61074.1"
                     /translation="MSELNFLFENNARWASDIKARDPDFFKKLVAQQTPEMLWIGCSD
                     SRVPANELLGLLPGELFVHRNIANAVVHTDLNCLSVLFYAVHVLKIKHIIVCGHYGCG
                     GVQAALGDKQVGLADHWLRNIRDVYYNRREQFTDELTERERINLLCELNVSQQVANVS
                     HSSVVQNAWDQGQDLSIHGWIYDIGDGLLKKITEVIDSPAQIPEQYRAKKQ"
     gene            complement(715..1332)
                     /gene="thrH"
                     /locus_tag="PKZ52_14720"
     CDS             complement(715..1332)
                     /gene="thrH"
                     /locus_tag="PKZ52_14720"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNG61075.1"
                     /translation="MEIACLDLEGVLVPEIWIKFSEKTGISELRATTRDIPNYDELMK
                     MRLAILAKHQLGLKEIQEVIATLSPLDGAIDFVNWLRERFQVVILSDTFYEFAGPLMA
                     QLGYPTLLCHKLTVDDKGMVVDYNLRQANPKRQAICAFKTLYYRTIACGDSYNDTTML
                     AEADAGILFHAPENVIREFPQFPAVHTYADLKQEFIKASIRPLSL"
     gene            1511..2233
                     /locus_tag="PKZ52_14725"
     CDS             1511..2233
                     /locus_tag="PKZ52_14725"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNG61076.1"
                     /translation="MNQIPLVDIEAKLLNASPFTIMEFALQEHGNIAISFSGAEDVAL
                     IDIASQIKPDIQVFCLDTGRLHPETYQFIERVRDHYKIAIDVLSPDGEAVRALVREKG
                     LFSFYQDDHAECCGIRKIAPLRNKLLTLDAWITGQRKDQSPGTRFNLPVVQLDPAFAR
                     PGETLTKYNPLANWTSQQVWDYIRAYQVPYNLLHERGFVSIGCEPCTKTVGPGQHERE
                     GRWWWEAATKKECGLHADNLKD"
     gene            complement(2316..2903)
                     /locus_tag="PKZ52_14730"
     CDS             complement(2316..2903)
                     /locus_tag="PKZ52_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG61077.1"
                     /translation="MNASAWNHRYKLVNLSAAPHCLPAIAAWQHDQWVQTLRPKQADL
                     FTPAPNIPADALQERQSQLRQHLGANLIPTTFVLLEKGRAVGSVSLVNYRRQNEKTQK
                     TKPLTTEVMDGQAGDKDAQSIWLTNMYVLPEHRKQGLAQVLLNRAEDYARSLQLKHIY
                     LYAQDAEGYYKKRGWLVLKTVNLDGQKLKVLVRHL"
     gene            complement(2952..>3321)
                     /locus_tag="PKZ52_14735"
     CDS             complement(2952..>3321)
                     /locus_tag="PKZ52_14735"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275799.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNG61078.1"
                     /translation="RLYREARPGANYAWSLKLLQEYKQRRPEVLTKSGLMVGLGETKE
                     EIIETMQHMREHQIDMLTIGQYLAPSKSHLPVKRYVEPAEFDDYARIAKELGFVHAAC
                     GPLVRSSYHADKQAHGELVK"
BASE COUNT          785 a          841 c          866 g          829 t
ORIGIN      
        1 actcccgagc tgattcaagc gaggtgcccc caatgagtga acttaacttt ttgtttgaaa
       61 ataacgcccg ttgggcgagc gacatcaaag cccgggaccc tgattttttt aagaaattag
      121 tggctcaaca aacgcctgaa atgctctgga tcggttgctc tgacagccgt gtgcccgcca
      181 atgagttatt aggtttattg cccggcgaat tatttgttca ccgcaatatt gccaacgccg
      241 tggtacatac ggatttaaac tgtttgtcgg tgttgtttta cgcagttcac gtcttaaaaa
      301 ttaagcacat tattgtctgc ggccactatg gctgcggtgg cgtacaagcg gccttgggcg
      361 ataaacaggt tggccttgcc gatcattggc tgcgcaatat tcgcgatgtt tattacaacc
      421 gccgggaaca gttcaccgac gagcttaccg agcgtgagcg gattaattta ttgtgtgaat
      481 taaacgtcag ccagcaggta gctaacgtca gccactcaag cgtggtgcaa aatgcctggg
      541 atcaggggca ggatttatca atccatggtt ggatttacga tattggcgac ggcttgttga
      601 aaaaaatcac cgaggttatt gattcaccgg cgcaaattcc cgagcaatac cgggctaaaa
      661 aacagtaatt gccgagctcg caaaagcgct cgataagagc gcttttgcgc cagattatag
      721 gctgagcggg cggatgctgg ctttgataaa ttcctgtttc aaatccgcgt aagtatgcac
      781 cgcaggaaat tgcggaaatt ctcgaattac attttcaggg gcgtgaaata aaataccggc
      841 gtccgcttca gccagcatgg tggtatcgtt gtaggaatcg ccgcaggcga tggtgcggta
      901 atacagggtt ttaaacgcac aaatagcttg gcgcttgggg ttggcttggc gcaggttgta
      961 atccaccacc atgcctttgt cgtccacagt gagtttatga cataacaaag tgggataacc
     1021 taattgcgcc attaaagggc cggcaaattc atagaaagtg tctgataaaa tcaccacctg
     1081 aaagcgctcg cgtagccaat tgacaaaatc aatggcgcca tctaatggcg atagagttgc
     1141 aattacctct tgaatttctt ttaaacccaa ttgatgtttg gccaaaatag ccaggcgcat
     1201 tttcatcaat tcatcgtaat tagggatgtc gcgggtggtc gcgcgcaatt cggaaattcc
     1261 cgttttctcg gagaatttaa tccatatctc aggcaccaat acaccttcaa gatctaagca
     1321 ggcaatttcc acaataagtc ctcatcagca agggtcaaca ataaaaggcc cgaaccttga
     1381 ggccgagcaa acgcgtctta cataaaggcc gccattctca ccaattcagc cagaggatgc
     1441 aatttttacc gcggcaattg cacgcggcct gggccatgat ggtattctgg gcttttgcgc
     1501 gggagccacc atgaaccaaa tccctcttgt cgatattgaa gccaaattgc tcaacgcttc
     1561 gccgtttacc attatggaat ttgccctgca ggagcacggg aatatcgcta tctcttttag
     1621 cggtgccgaa gatgtggcac tgattgatat tgccagccaa ataaagcctg atattcaggt
     1681 gttttgctta gacacaggcc gcttacaccc tgagacctat caatttattg agcgcgtgcg
     1741 cgatcactac aaaatcgcca tcgatgtact cagccccgat ggcgaagcgg tgcgtgccct
     1801 ggtgcgcgaa aagggcctat ttagctttta tcaggacgat cacgccgagt gctgcggcat
     1861 tcgcaaaatt gccccgctgc gcaataaatt gctgacactg gatgcctgga tcaccggcca
     1921 gcgcaaagat cagagccccg gcacccgctt caatttaccg gtggtacaac ttgacccggc
     1981 ctttgcccgc ccaggcgaaa ccctcactaa atacaatccc ctagccaact ggacttccca
     2041 acaggtgtgg gattacatcc gcgcctacca ggtgccttac aacctgttac acgagcgggg
     2101 ttttgtgtcg attggctgcg aaccgtgcac caaaacagtt gggcccggcc aacatgagcg
     2161 ggaggggcgc tggtggtggg aagcggcgac gaaaaaagaa tgtggtttac acgctgataa
     2221 tttaaaagat taagcagacg cgcttatcaa ctcctgaacc gctgaaaatt gtgcaattgt
     2281 ttttatgcaa atgacatttt tcagcggttc gcactttata aatgccgcac caaaaccttt
     2341 aatttttgcc cgtctaaatt caccgttttt aacaccagcc agccgcgttt tttgtagtag
     2401 ccctcggcgt cttgggcgta gagataaatg tgtttgagct gcaggctgcg ggcgtagtct
     2461 tcggctcggt tgagcagtac ctgggcaagc ccctgtttgc ggtgctccgg caacacatac
     2521 atattggtga gccaaatact ctgggcgtct ttatcacccg cctgcccatc catgacctcg
     2581 gttgttagcg gcttagtttt ttgtgttttt tcattctggc ggcggtaatt caccaggctt
     2641 accgagccta ccgcgcgacc cttttccagc agtacaaagg tggtgggaat caggttggcg
     2701 cctaggtgtt ggcgcagttg ggattgccgc tcctgcaggg cgtcagcggg aatattcggc
     2761 gcgggggtga ataaatcggc ctgtttgggc cgcagggttt gcacccattg gtcgtgctgc
     2821 caggcagcta tggcaggcag gcagtgggga gccgctgaga ggttgacgag tttatagcgg
     2881 tgattccaag ccgatgcatt catctgcgca tcaccacccg ctgctgtatc accgccacgg
     2941 gttgaacccc cctatttcac cagctcaccg tgggcctgtt tatccgcgtg gtagctcgag
     3001 cgcaccaggg ggccgcaggc ggcgtgtaca aagcccagct ctttggcaat gcgggcatag
     3061 tcgtcgaatt cagcgggctc cacataacgc ttcaccggca agtgggactt agacggcgcg
     3121 aggtactggc cgatggtgag catgtcgatt tgatgctccc gcatgtgttg catggtctcg
     3181 ataatttctt ctttggtctc ccctaaaccc accatcaaac cggatttggt taacacctcg
     3241 ggcctgcgct gcttatattc ctgcagcaat tttaatgacc aggcgtagtt ggcgccgggg
     3301 cgcgcttccc gatacaggcg g
//
LOCUS       DAOQGS010000464         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16790_length_3310_cov_4.680799, whole genome shotgun sequence.
ACCESSION   DAOQGS010000464 DAOQGS010000000
VERSION     DAOQGS010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16790_length_3310_cov_4.680799"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKZ52_14740"
     CDS             complement(<1..546)
                     /locus_tag="PKZ52_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HNG61079.1"
                     /translation="MFNLKTIMPGGVHPDGHKDATATRPILTRIPLPPRLYLPLGQQA
                     GEQSRPLVKVGEQVLKGQRIAISGGSLSSNIHAPTSGTVVAIDDITAPHPSGLPTRAI
                     VIESDGEDRWIELTEHLNPKELSPEELATKVEEAGIVGMGGAIFPAAVKLRQGRRYEI
                     KTLLVNGGECEPYLTTDDRLMR"
     gene            complement(585..1130)
                     /locus_tag="PKZ52_14745"
     CDS             complement(585..1130)
                     /locus_tag="PKZ52_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNG61080.1"
                     /translation="MFIATFVLTLLGLSLGLLLGIAAKVFAVGDENPLVKEVEGLLAG
                     SQCGQCGYPGCGPAAKAIVDGDAPITCCPPGGKSVIEALAKCLDIDLNSLGEVAGPKI
                     ARIEKELCVGCTRCFKVCPTNAIVGANKQLHVVMEKACIGCSKCAEECPEDCIAMIPE
                     PVTLNNWHWPKPELVPLAASA"
     gene            complement(1145..1717)
                     /gene="rsxA"
                     /locus_tag="PKZ52_14750"
     CDS             complement(1145..1717)
                     /gene="rsxA"
                     /locus_tag="PKZ52_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNG61081.1"
                     /translation="MDYLLLFISTTLVNNMVLVKFLGLCPVMGVTNKLDSAIGMGLAT
                     TFVITVSSLCAWVIDELLLIPMHIEYLRILSFILLAAAVVQFTEAVMRKASPLLHKSL
                     GIYLPLIVTNCAVLAVPLLNTSLHHTLGQSLIYAVGSSVGFTLVMVIFAGLRERLAIA
                     NVSETFKGTPIGLITAGLLSLIFMGFSGMI"
     gene            1973..2143
                     /locus_tag="PKZ52_14755"
     CDS             1973..2143
                     /locus_tag="PKZ52_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61082.1"
                     /translation="MSEIAETMPRTFLAKIVGWGIFLALFCRNSAADNAVLSGCCVGF
                     ATNKNRPERGHL"
     gene            2293..>3310
                     /gene="nifL"
                     /locus_tag="PKZ52_14760"
     CDS             2293..>3310
                     /gene="nifL"
                     /locus_tag="PKZ52_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HNG61083.1"
                     /translation="MRKLGQPLPDPSGLLVASKKIAQGLLPEQELLSEEVFFQAVEHC
                     PVAISITDLKANILYANRMFTQVTGYAMEEVIGKNESILSNHTTPRLAYQALWGRLAQ
                     QKPWAGMLLNRRKDGSLYLAELTVAPVINEKGETVNYLGMHRDCTEIHQLEQRVLNQK
                     AMIEAVINAAPEAMVVLDEQGKVVLSNPSFVAIAADLAPDKTQETLLSILGEKLDGKL
                     EPLLQNGQGFTSKEITIEQGSYTPRYFSCFGTCIDVQGETADGFFEQTVIHYKMLVFN
                     DITDVRRRQRDTHLTALKALMAEQELVQGMRETLNGAIHQLQGPVNVFAAAVTIMQRR
                     AVANR"
BASE COUNT          814 a          836 c          904 g          756 t
ORIGIN      
        1 gcgcatcagg cgatcgtcgg tggtgaggta gggttcgcac tcgccgccgt ttaccagcag
       61 ggttttaatt tcatagcggc ggccttggcg cagtttcacg gccgccggga atatagcccc
      121 gcccatgccc acgatgcccg cttcttccac cttggtggcc aactcctcag ggcttagctc
      181 tttgggattg aggtgttcgg tcagctcaat ccagcggtcc tcgccatcgc tttcaatcac
      241 tatggcgcgg gtgggcaagc cggaagggtg gggagcggtg atgtcgtcga tggctaccac
      301 agtgccggag gtgggggcgt gaatattgga ggacaggctg ccgcccgaaa tcgcaatacg
      361 ctggcctttg agtacctgct cgcccacctt aaccaggggg cgcgattgct cgccggcctg
      421 ctggcccaag ggtaaataaa gcctgggcgg caggggtatt ctggtgagta tggggcgggt
      481 ggcggtggca tccttgtggc cgtccgggtg cacgcccccc ggcataatgg ttttgaggtt
      541 aaacataagc gctacttctg atacggcagg taaatgcagg cgagttaggc ggacgcggcc
      601 aagggcacca gttcaggttt gggccagtgc cagttgttga gggtgaccgg ctcgggaatc
      661 atggcaatgc aatcctccgg gcactcctcg gcgcatttgg agcagccgat acaggctttt
      721 tccatcacta cgtgcagctg tttattggcg cccacaatgg cgttggtggg gcagactttg
      781 aagcagcggg tgcagcccac gcagagttct ttttcgatgc gggcaatctt gggcccggcc
      841 acttcaccca ggctgttcag gtcgatatcc aaacacttag ccagagcctc gattacgctt
      901 ttgccgccgg gtgggcagca ggtgatgggc gcgtcgccat cgactatggc tttggccgca
      961 ggcccgcagc cggggtagcc gcactggccg cactggctgc cagccaatag gccctccact
     1021 tctttaacca gcggattttc atcccccacg gcaaatactt tggcggcgat tcccaacaat
     1081 aaacccagtg ataaccccag cagggttaaa acgaatgtag cgataaacat ggcgtgttgc
     1141 cctcttaaat catgccgcta aagcccataa aaatgagcga taacaagcct gcggtaatta
     1201 atccaatcgg tgtgcctttg aaggtttcac tcacattagc tatggccaga cgctctctga
     1261 ggcctgcaaa aatcaccata acgagggtaa aaccaaccga tgagcccacc gcataaataa
     1321 ggctttggcc tagggtgtgg tgcaaactgg tattgagcag cggcacggct aacaccgcgc
     1381 agttggtaac aatgagcggt aaataaatgc cgagggattt atgcaaaagt gggctggctt
     1441 tgcgcatcac cgcctcagta aattgcacca ccgcagcggc caataaaatg aaggataaaa
     1501 tgcgcaaata ttcgatatgc atggggatta acaataactc gtcgattacc caagcacata
     1561 aagatgaaac ggtgataaca aaggtggtag ccaggcccat cccgatggcg gaatccaatt
     1621 tattggttac acccatcaca gggcagaggc ctaaaaattt caccaacacc atgttgttta
     1681 ctagggtggt gctgataaaa agcagtaaat aatccatacc cgtatctcaa ctattggggt
     1741 taaacacagt gctttcgcaa ggacacaggc tggctgtgca gcgcggtttt tcagcgcgat
     1801 ttacagtggt gaaattccgg ccattaaact tcttactagc tgtgcaaaag caatgaatac
     1861 acatcatttc agaccatcaa aaaaagggac ctttttgacg agcctacacg gatgtattca
     1921 cggcgtgtct gaaatgatgt gtattcattg cgggtgtcta ggtagtaagc gtatgagcga
     1981 aattgcagaa actatgccac gcaccttttt ggcgaaaatt gtcggttggg ggattttttt
     2041 ggctttattt tgtcgcaaca gcgccgccga caatgccgtt ttgtcgggct gttgtgtggg
     2101 ttttgcgaca aataaaaatc ggcccgagcg cggccatttg tagtatttgc gacaaatgcg
     2161 acaggcttgt agcgcttgcc cccagtaaat aggcctttgt cagttgggca cacattttgc
     2221 ccctgcccgt tagcacaccg ccggatgaaa ttttttaggt atttaggcgg gttatcagcc
     2281 agaggacaga atatgcgtaa acttgggcag ccacttcccg atccgtcagg cctattagtg
     2341 gccagcaaaa aaatcgccca aggcctgctg cccgagcaag agttattgtc ggaagaagta
     2401 ttttttcaag cggtggagca ctgcccggtg gccatctcta ttaccgattt aaaagccaat
     2461 attctctatg ccaaccgcat gtttacccag gtgaccggtt atgccatgga ggaggtcatc
     2521 ggcaaaaacg aatccattct ctccaaccac accacccccc gtttagccta ccaagccctt
     2581 tgggggcgcc tggcgcaaca aaaaccctgg gcgggtatgt tgttaaaccg ccgcaaagac
     2641 ggctctttat atctagcgga gttaactgta gcgccggtca ttaatgaaaa gggcgaaacc
     2701 gttaattatt taggcatgca tcgggactgc acggagatac atcaattaga gcagcgggta
     2761 ctcaaccaaa aagccatgat tgaagcggta attaatgccg cccccgaagc catggtggtg
     2821 ctggatgagc agggcaaagt ggtgttatcc aaccccagtt ttgtagccat tgcggcggat
     2881 ttagcgccgg ataaaaccca agaaacactg ctgagtattt tgggtgaaaa actggacggt
     2941 aaattagaac ccttattgca aaacggccag ggatttacca gtaaagaaat taccatcgag
     3001 cagggcagct acacgccccg ttatttttcc tgttttggca cctgtattga cgtgcagggc
     3061 gaaacggccg acggcttttt tgaacagacc gttattcact acaaaatgct ggtatttaac
     3121 gatattaccg atgtgcgcag gcgccagcgg gacacccatt taaccgcctt aaaagcgctg
     3181 atggcggaac aagaattagt gcaaggcatg cgcgaaacac tcaatggcgc cattcaccaa
     3241 ttgcaagggc cggttaatgt atttgccgcc gcagtaacca ttatgcagcg gcgggcggtg
     3301 gccaaccggg
//
LOCUS       DAOQGS010000465         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_16929_length_3295_cov_4.315123, whole genome shotgun sequence.
ACCESSION   DAOQGS010000465 DAOQGS010000000
VERSION     DAOQGS010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16929_length_3295_cov_4.315123"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKZ52_14765"
     CDS             complement(<1..234)
                     /locus_tag="PKZ52_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNG61084.1"
                     /translation="MRLTTKGRYAVTAMLDLALHANAGPVSLADISGRQEISLSYLEQ
                     LFAKLRQNQLVQSVRGPGGGYRLGGPQDQIFVAE"
     gene            complement(307..1116)
                     /gene="trmJ"
                     /locus_tag="PKZ52_14770"
     CDS             complement(307..1116)
                     /gene="trmJ"
                     /locus_tag="PKZ52_14770"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HNG61085.1"
                     /translation="MTAHCFDRIRIVLVNTTHPGNIGGAARAMKNMGLARLVLVAPKE
                     FPSERALWRAASAQDVLEKVQVVDTLEEAVADCALVLGTSARERRIPWPLLTPRQSGE
                     RVITEAAAGHEIAIVFGREDRGLTNEELQLCQFHIHIPSNPDYSSLNLAAAVQVIAYE
                     LRVHWLERQAGRELVFDDWDQPPAASKDLELYFQHLEQTLTELNFLTPDNPRQTMTRL
                     RRLFNRVRLDQMELSILRGMLTGIQNFIYHSQKRLAKLQAGTKPADDSIPD"
     gene            1279..2073
                     /locus_tag="PKZ52_14775"
     CDS             1279..2073
                     /locus_tag="PKZ52_14775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNG61086.1"
                     /translation="MEPMLTMALRAARKAGEIIERAIERVDLLTIEEKGRNDFVSEID
                     KKAEQEILYHLKKAYPDHAFLGEETGRSNSSDYVWIIDPLDGTTNFLHGIPHFSVSIA
                     CAYKGVLTHAVVYDPIKREEFTASKGRGAHLNDRRIRVSNRRSLDGALIGTGVPFNGF
                     ALEHIDDYLNCLREVAGQTAGVRRAGSAALDLAYVAAGRFDGFWEMNLKEWDMAAGIL
                     LIKEAGGMVSDFKGGNEYLEQGHVVCAAPKVFKPLLQIVGKHMGHI"
     gene            2449..>3295
                     /locus_tag="PKZ52_14780"
     CDS             2449..>3295
                     /locus_tag="PKZ52_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNG61087.1"
                     /translation="MVLIVRILLLCSFLTALAPQARAANTTAHAIQYFDLPAQNLAAG
                     LIEFALQAHITLVVDDQLVAGLYGQPVNGRLSVETGLQQLLQGTDLTFSYQAQSNSYL
                     LQKNLASPAPATTAAQTEIEEIVVLGYLTYPFRYTTVRNSQLQAGVNYFDSVRFTNVL
                     PQQLISDQKPEDLADVLKFASGVMPADGLADTNDDIYIRGFGRAAIFFDGLRIGDTTG
                     TKLLPAVIERVELLKGPGTLFFGQAEPGGTLNFISKKPTAESFINSEITAGTLGKKHY
                     TLDINR"
BASE COUNT          784 a          900 c          855 g          756 t
ORIGIN      
        1 ctccgcaacg aatatttgat cctgtggccc gccgagccga tagccgccgc cggggccgcg
       61 cacactctgc accaactggt tctgccgcaa cttggcgaac aactgctcaa ggtacgaaag
      121 ggaaatctcc tgccgcccgg aaatatcggc aaggctcacc ggccctgcat tggcgtgtag
      181 ggctaggtcc aacatagctg tcaccgcgta gcggccttta gttgtaagtc gcatacttat
      241 gcctcctgat ctgtccttaa attatcaaat acccgagtgt tttaatcaag taaatatcct
      301 agtcttttaa tcgggtattg agtcatccgc aggcttagta ccagcctgta acttggccag
      361 acgtttttgg ctgtgataaa taaaattctg aatccccgtc aacatgccgc gcagaatgga
      421 caactccatc tggtcaaggc gcacccggtt aaacaaacgg cgcagacggg tcatggtttg
      481 gcgcgggtta tccggggtta aaaaatttaa ctccgtcaga gtctgctcaa ggtgctgaaa
      541 atacagctct aaatctttgg aggcggctgg cggctgatcc caatcgtcaa acaccaactc
      601 ccgccccgcc tgccgctcca accagtgcac acgcaattca taggcgatca cctgcacagc
      661 ggcagccagg ttaagggagc tgtagtcggg gttggaggga atatgaatgt ggaattggca
      721 caattgcagc tcttcgttgg tgaggccgcg atcttctcgg ccaaacacta tggcgatctc
      781 atggcccgcc gccgcttcgg taatcacccg ctcaccactc tggcggggcg ttaacaaggg
      841 ccagggaatg cgccgctcgc gggcactggt gccaagcacc aaagcgcaat cggccacggc
      901 ttcctccagg gtatccacca cctggacctt ttctaataca tcctgggcgc tggccgcccg
      961 ccacagggcg cgctcggacg gaaactcctt gggcgccacc aataccaagc gggctaggcc
     1021 catattcttc atggcccgcg ccgcgccgcc gatgttgccg gggtgggtgg tatttaccaa
     1081 cacaatgcgg atgcggtcaa aacaatgggc agtcataggg cggtggtcac agcgtaacgt
     1141 taggggggcg caaaaaagcc gcgcagtcta gcagaaggcg gccgccaggc gaagcggcgg
     1201 cgggcaggag tttcgctggc tggcaaggcc cctcgctgct acaatgcgcg gctttttgcc
     1261 cctaccttga gttacaccat ggaacccatg ttaacaatgg ctcttagagc cgcccgtaaa
     1321 gccggtgaaa ttattgagcg cgccatcgag cgcgtggatt tactgaccat cgaagaaaaa
     1381 ggccgcaacg attttgtcag cgagattgat aaaaaagccg agcaggaaat cctctaccac
     1441 ctaaaaaagg cctatcccga ccacgcgttt ttgggcgaag aaaccggccg cagcaacagc
     1501 agcgactatg tatggatcat cgacccgctg gatggcacga ctaacttcct ccacggcatt
     1561 ccccattttt ccgtttccat cgcctgcgcc tacaagggtg tgctgaccca cgcggtggtc
     1621 tacgacccga tcaaacggga agaatttacc gccagcaaag gccgcggcgc gcacttaaat
     1681 gaccgccgta tccgcgttag caaccgccgc agcctagatg gtgccttgat cggtaccggc
     1741 gtgcctttta acggcttcgc gctggaacac atagacgact acttaaattg tttgagggaa
     1801 gttgcaggcc aaaccgccgg tgtgcgcagg gccggttccg ctgcgcttga tttagcctat
     1861 gttgccgctg gccgttttga tggcttttgg gagatgaatt taaaagaatg ggatatggcg
     1921 gcgggcattt tgttaattaa agaagccggt ggcatggtca gcgattttaa aggcggcaat
     1981 gaatatttag agcaaggcca tgtggtgtgc gcagctccta aggtctttaa gccgctgctg
     2041 caaattgtcg gcaaacacat ggggcatatc taacccacct acaaggcttt gttatgccct
     2101 aaaaattgct gcaccgaggg cgcggaattt ttagggcatt tatctgcgca cttaggtttt
     2161 tagcccccgc gcgccaaacg ccccccctat ctgccagctc cccagtaaaa atgtccgcta
     2221 acactcagaa ttcacacaca ggatgggtta cggatttttt ttgcggctac aatgctcagc
     2281 gcggttgtaa ccgggaatat ttgggtgctc aagcaggcaa cccaaaaaac tgtggtgaat
     2341 agttaggccc attttttggg cctggttttt ttagctattg atcatagtgc gttttaagga
     2401 ggcgttatgg tgcgcctaaa aaccaagttg cgttaggctt gttaggctat ggtgttaatc
     2461 gttcgaatcc tcctgttgtg ctcattttta acggctttag cgccccaggc ccgcgccgcc
     2521 aacacaaccg cccatgctat tcaatatttt gatctacctg cgcaaaatct cgccgcaggc
     2581 ctaattgaat ttgctctaca ggcacacatc actttagtag tagacgatca actggtggcg
     2641 ggtttatacg gccagccggt taacggccgc ttaagcgtgg agacaggcct gcaacaactt
     2701 ctgcagggca ccgatttaac ctttagttat caggcgcaga gcaattctta tttactgcaa
     2761 aaaaacctcg ccagccccgc acctgcgaca acggctgcgc aaactgaaat tgaagaaatt
     2821 gtcgtactgg gttatctcac ctatccattt cgctacacta cggtgcgcaa cagccagctg
     2881 caggccggcg tgaattattt tgattcggtg cgctttacca atgtattgcc gcaacaatta
     2941 atcagcgatc aaaagccgga agacttagca gatgtattaa aattcgccag cggcgttatg
     3001 cctgcggatg gcttagccga caccaacgat gatatttata ttcgcggctt tgggcgcgcc
     3061 gctatctttt ttgatggttt gcgcattggc gataccacag gcacaaaatt attaccggcg
     3121 gttattgaac gggtcgaatt gttaaaaggc cctggcactc tttttttcgg ccaggccgaa
     3181 cccgggggca cacttaattt catcagcaaa aaacccacag cagaatcttt tatcaacagc
     3241 gaaataaccg ctggcaccct cggcaaaaag cactatacgc tggacatcaa ccgcc
//
LOCUS       DAOQGS010000466         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17013_length_3281_cov_5.744885, whole genome shotgun sequence.
ACCESSION   DAOQGS010000466 DAOQGS010000000
VERSION     DAOQGS010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17013_length_3281_cov_5.744885"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PKZ52_14785"
     CDS             <1..1288
                     /locus_tag="PKZ52_14785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNG61088.1"
                     /translation="RRYRRQKHLNAKLRELDSLKDDFMANTSHELRTPVNGIIGIAQA
                     LQDGIAGNQSATTLKHIDMILNCGRRLERLINDVLDFSTAKTASLQLVRSCFNITSVI
                     AEVVHECTPNLAADQVQLINRATGEYPLLFGDAERTRRVIYNLLTNAIKYTERGTISI
                     DVTASADALCIIVQDTGIGISEEQLPNIYESFTQIASSGALTKNGTGLGLTIAKYFVE
                     AQGGALQVHSKPGEGTTFSVWLKQASADEINQPLAKPPNTPATPAAVPAGEIQPPDSP
                     AHPHHESLLPVFYPKNYQPNLSLKKILVVDDEAINRTILRSQGLKYGFIVYEAANGQE
                     AIDALYMGLSCDLILLDIMMPKLSGIETCRLIRNRYSREQLPIVFVSAKTQPCDIDEC
                     FRAQGNDFLAKPVNREELISCMEKYLEQSPINKLAS"
     gene            complement(1742..>3281)
                     /locus_tag="PKZ52_14790"
     CDS             complement(1742..>3281)
                     /locus_tag="PKZ52_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNG61089.1"
                     /translation="QQYVAGGWPSMTHPVEYGGQGLPVSMETLVSDMVGAANWAWGMY
                     PGLSHGAMHTLSLHGTDEQKATYLTKLVAGEWTGTMCLTESHCGTDLGMLRTKAEPQA
                     DGSYRISGSKIFISAGEHDLTENIVHIVLARIPGAPEGTKGISLFIVPKFLPDAAGAV
                     GLRNAVSCASIEHKMGIHANATCVLNFDGATGFLIGEPNRGLHYMFTFMNNARLGTAV
                     QGLSHAQLGFQKSLSYAKERLQSRSLSGVKNPAGPADPIIVHPDVRRMLLTQKAMAEG
                     GRMLAYSTALLVDKDAFASDEDQRKEASELLALLTPIAKAFMTETGFECASLALQCFG
                     GHGYIQEWGVEQNLRDSRIAMLYEGTTGIQALDLLGRKILMTQGESLKRFTKIIHKYC
                     KSQEGNDQMAEFIEPLAAKNKEWGELTLQIGMKAMSNPDEVGAAAVDYLMYSGLVTFA
                     YWWARAAEIALNKQDEPFYAAKLTTARFFYQRLLPRTLAHAAAMKTGVDGLLALDAEH
                     FSFD"
BASE COUNT          837 a          870 c          803 g          771 t
ORIGIN      
        1 ccgccgctat cgcaggcaaa aacacctcaa tgccaaattg cgggagctgg attcattaaa
       61 agatgatttt atggcgaata cctcccacga actgcgcaca ccggtcaatg gtattattgg
      121 catagcccag gcgttgcagg acggtattgc gggcaatcaa tcggcaacca cactcaaaca
      181 cattgatatg atcctcaact gtgggcgccg cttggagcgt ttaattaatg atgtgttgga
      241 tttctccacc gccaaaaccg cttccctaca attggtgcgc tcctgtttta atatcacctc
      301 ggtaattgcc gaagtagtac acgaatgcac accaaattta gccgctgacc aagtgcaatt
      361 aatcaaccgc gccactggcg agtacccgct tttatttggc gatgccgaac gcactcggcg
      421 cgtaatctac aatctgctga ccaatgcgat taaatatacc gagcgcggca ccattagcat
      481 tgatgttact gcctcagccg atgcgctatg catcattgtt caagatacgg ggatcggtat
      541 tagcgaagag caattaccga atatttatga atcctttacc cagatagcgt cctccggcgc
      601 attaacgaaa aatggcaccg gcttgggttt aaccatcgct aagtattttg ttgaggcaca
      661 gggtggtgct ttacaagtgc actcaaaacc gggcgaaggc actaccttta gcgtgtggct
      721 taaacaagcc agtgccgatg aaattaacca acccctggca aagcccccca atacgcccgc
      781 aaccccagca gcggtacccg caggggaaat acagccaccc gacagccctg cacaccctca
      841 tcacgagagc ctgctaccgg tgttttatcc aaaaaattac cagcctaatt tatcgttgaa
      901 aaaaattctg gtggtggatg acgaagctat caaccgcacc attttacgca gccagggcct
      961 taaatatggc tttattgtct atgaagcagc caatggccag gaggccattg acgcgcttta
     1021 tatggggctc agctgcgatt taattttgct ggatattatg atgccgaaat tatccggcat
     1081 tgaaacctgc cggttaattc gcaaccgcta ctcccgcgaa cagttgccga ttgtctttgt
     1141 tagcgccaaa acccagcctt gtgacataga cgagtgtttt cgcgcccagg gcaatgattt
     1201 tttagccaaa cccgtcaacc gcgaagaact tatttcctgc atggaaaaat acctagagca
     1261 gtctcccatt aataaactgg ccagttaatt aaccgcaacc catcagcacc ttttaatccg
     1321 aaccaagata aatccggcga cgctccttac cgcctaggca ccggcaatga atacacatcg
     1381 tttcagacag gccgtgaata caatccctgt aggcttgtca aaaaggtccc tcttgttgac
     1441 ggtctgaaat gatgtgtatt cattgcttcc gtaaagcgag taaataagca ctttattaac
     1501 ccggcactaa atgccgccgg gttaataaca ccatgctagc ggcccacatc ttggcaacag
     1561 gctgcaagcg acgaaaggcc aaataaaaat acattagaat gtactgaacg gttagggcca
     1621 aacataaatg gatcctggga ctgcgcgcag gatgacgggt ggtttacgca ggatattcac
     1681 gccatgggag cgcccacaaa aaaggctgcc gcagcagcct tttttattca tttagccgtg
     1741 tttaatcaaa cgaaaaatgc tccgcatcca gtgccagcaa accatccaca ccggttttca
     1801 tggccgctgc atgggctagg gtgcggggca ataaacgctg atagaaaaaa cgcgcggtag
     1861 tgagtttagc ggcgtaaaaa ggctcatctt gtttattcag ggcaatttcc gctgcccgcg
     1921 cccaccagta ggcaaaggtg acaaggcctg aatacattaa ataatccact gcagcagcgc
     1981 caacttcatc cgggttgctc atggccttca tgccaatctg cagggtgagc tcgccccact
     2041 ctttattttt ggcggccagc ggttcaataa attccgccat ctggtcattg ccttcctggc
     2101 ttttgcagta tttgtgaata attttggtaa agcgttttag gctttcgcct tgggtcatta
     2161 aaattttgcg gcccaataaa tccaacgcct gaataccagt ggtgccctca tacaacatgg
     2221 caatacggct atcgcgcaga ttctgctcca caccccactc ttgaatataa ccgtggccac
     2281 caaaacactg cagggcaagg cttgcgcatt caaacccagt ttcggtcata aaggctttgg
     2341 ctatgggtgt taacagggcc aataattccg aggcttcttt acgctgatct tcgtcgctgg
     2401 caaaagcgtc tttatccacc aacaaagcgg ttgaataggc gagcatacgg cctccctcgg
     2461 ccatagcttt ttgggtaagc aacatgcggc gcacatccgg gtgcacaata atcgggtcgg
     2521 cgggacctgc agggtttttc acaccggata aagagcgcga ttgcaaacgc tctttggcgt
     2581 aacttaagga tttttgaaat cccaactgcg catggcttag gccctgcacc gcggtgccca
     2641 agcgggcgtt gttcataaag gtgaacatat agtgcaagcc ccggttgggc tcgccaatta
     2701 aaaagccggt ggcgccatca aaatttaata cacaggtggc gttggcgtgg atacccattt
     2761 tgtgttcgat ggaggcgcag ctcactgcat tgcgcaggcc cacagcacct gcggcatcgg
     2821 gtaaaaattt cggcacaata aataaagaaa tgcctttagt gccctccggg gcaccgggga
     2881 tacgcgccag cacaatatgc acgatatttt cggtgagatc gtgctcgcca gcggagataa
     2941 aaattttgct gccgctgatg cggtagctgc cgtcggcttg aggctcggct ttagtgcgca
     3001 gcatgcctag gtcagtgccg cagtgggatt cggttaaaca catggtgccg gtccattcac
     3061 cggccacgag cttggtaaga taagtggctt tttgctcgtc ggtgccgtgc aaactcaggg
     3121 tgtgcatggc gccgtgggag agacctgggt acatgcccca agcccagttg gcggcgccca
     3181 ccatatcgct caccagggtt tccatggaga ccggcagccc ctggccgccg tattccaccg
     3241 ggtgggtcat ggagggccaa ccgccggcta catattgctg g
//
LOCUS       DAOQGS010000467         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17016_length_3281_cov_3.891507, whole genome shotgun sequence.
ACCESSION   DAOQGS010000467 DAOQGS010000000
VERSION     DAOQGS010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17016_length_3281_cov_3.891507"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PKZ52_14795"
     CDS             <1..1418
                     /locus_tag="PKZ52_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNG61090.1"
                     /translation="RDVVGWLKCEYLQSRVGEVFPGIVGAVVGFGLFVELSDLFVEGL
                     VHISSLPQDYYHHQPAQHRLVGERTGRVFRLGDRLTVRVVRVSLDERKVDFELVEHLG
                     KSVPNAKFNRDKKSAKRTAFVPHEHPAKKQQPVYAEQLAKSGGKKKKKQKLSKAERGA
                     KGKSQDFSAATKVEPINQAANKASIKGGIAGATKPVNTSAATIRKRAATAAPIETANT
                     KTPGKVAAAQLNSSVKAPNKSNEQERVTSKVGSAAQAVAPLASAKKTSRKEKPKSSAK
                     NSATSAQQAVAPVINAEKAKAKVKVAAPVTSAAKAKQKPSSATAAKATKKTAAGTAKK
                     AAAAVKPAANKKALAAKKPARAEKPTITPKAGANKIAAADKKSPANKKAKAGAKLAVT
                     KKSPAAATGKNSAPTKSVGTKKTALAKATAAKSGGATAKAGQSKPKPAVKTSKTSPKA
                     AAVKKPALKVKNKVSASKTKK"
     gene            1513..2256
                     /gene="rlmB"
                     /locus_tag="PKZ52_14800"
     CDS             1513..2256
                     /gene="rlmB"
                     /locus_tag="PKZ52_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNG61091.1"
                     /translation="MAKSEYVFGLHAVQSLLKTAPLRIEKLFVLRGREDQRIQKVLNL
                     AQAQDISWEWQTRKEMDARSGEGNHQGVIAVAKPGQLQDETFLYQLIADAAPILLLVL
                     DGVTDPHNLGACLRSADAAGVQAVIIPKDNSAGLTETARKVACGAADSVPLVSVTNLS
                     RCLEKLQELGVWLVGTTGDAKDTIYDLDLTGNIAVIMGAEGSGMRRLTREHCDYLAHI
                     PMCGSVSSLNVSVATGVCLFEALRQRRQS"
     gene            2299..>3281
                     /locus_tag="PKZ52_14805"
     CDS             2299..>3281
                     /locus_tag="PKZ52_14805"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNG61092.1"
                     /translation="MRLCVFSSKPYDEQYLRPALEGECELVFLPVHLNKNTVPLAEGA
                     GGICCFVNDDLSAPVLEALALRGIQFIVLRCAGFNQVDVAAAKRLKLVIARVPEYSPY
                     AVAEHAVALILALNRNIHRAHNRVREGDYSLQGLMGFDLHGKTVGIVGAGKIGRAFAA
                     IVRGFGCTVLICDPVADPASCDWARWVDFDSLMQQSHIVSLHCPLTPHTHHLINARAL
                     ALMQPGAMLINTSRGALVDARAVIDALKSGHLGYLGLDVYEEEGDLFFEDLSNQVIQD
                     DVFARLTTFPNVLITAHQAFFTHEALTRIAEVTLLNLRAIKQGALIPANLVA"
BASE COUNT          879 a          736 c          888 g          778 t
ORIGIN      
        1 cccgcgatgt ggtgggatgg cttaagtgtg aatatttaca aagccgagta ggggaggttt
       61 tccccggtat tgtgggcgcg gtggtgggct ttggtttatt tgtggaattg agcgatttat
      121 ttgttgaagg cctggtgcat atttcatccc taccgcagga ttattatcac caccaaccag
      181 cgcaacaccg tctggtgggg gagcgcaccg gacgagtatt tcgtttgggg gatcgtttaa
      241 cggtgcgagt ggttcgtgta agcctagatg agcgtaaagt cgattttgaa ttggtggagc
      301 atctgggcaa aagtgtgccc aatgctaaat ttaatcgcga taaaaaatcg gcgaagcgca
      361 cagcgtttgt gccgcatgaa catccggcta aaaaacagca gcctgtttat gccgaacagc
      421 tagcaaagag cggcggcaag aaaaagaaga aacagaaatt atccaaagcc gagcgtggcg
      481 ctaagggtaa atcgcaggat ttttctgccg ctacgaaagt agagccaata aaccaagcgg
      541 ctaataaggc gagtattaaa ggtggtattg ctggggcaac taagccggtc aataccagcg
      601 cggcaaccat tcgcaagcgc gccgcaaccg ctgctcctat agagactgcc aatactaaaa
      661 cgcccggcaa agtagcggcg gctcaattaa acagctcagt taaggcgcca aataagtcga
      721 atgagcaaga acgagtaaca agcaaagtag gttctgccgc tcaggcggtg gcacctctgg
      781 cgtctgcaaa gaaaacctcg cgcaaagaga agccaaaaag ctcagccaaa aatagcgcaa
      841 ctagcgcgca acaggccgtt gcgccggtaa ttaatgctga aaaggctaaa gcaaaagtta
      901 aggtggcggc acctgtgact tcagctgcta aagcgaagca aaagccatcg tcagcaacgg
      961 ctgctaaggc aactaagaaa acagccgcag gcacagctaa aaaagccgcc gcggcagtaa
     1021 agcccgccgc aaataaaaaa gcgctggcag cgaaaaagcc agcaagagcg gaaaagccaa
     1081 caattacccc aaaggctggt gccaataaaa ttgcggcggc agataaaaaa tcaccagcaa
     1141 acaaaaaagc caaagcgggg gcaaagcttg ctgtaactaa aaagtccccc gctgcagcaa
     1201 caggcaaaaa ttccgcccca actaaatctg ttggcacaaa aaaaacagcc ctggcaaaag
     1261 ccaccgccgc caaaagtggt ggtgcaaccg ccaaggcggg gcagtctaaa cccaagcctg
     1321 cagtaaaaac ctcaaaaaca tcacctaagg ccgccgcagt gaaaaagcct gccctcaaag
     1381 tgaaaaacaa ggtatccgcg agtaaaacaa aaaaataagt ttttatcgtt gatgatatgt
     1441 gcacgtattt tgggatgtaa acagcgccct gggtgatcgg atcctcaggg ctttcttttt
     1501 agagcaatag gtatggcaaa atctgaatat gtatttgggc tgcacgccgt gcagtcacta
     1561 ttaaaaacgg cgcctttgcg cattgagaaa ttatttgtat tgcgcgggcg cgaagatcag
     1621 cgcatacaaa aagtacttaa tttggcgcag gcgcaggata ttagttggga atggcaaacc
     1681 cgtaaggaga tggatgcccg cagtggcgag ggcaatcatc agggtgtgat tgcagtggct
     1741 aaaccggggc agctgcagga tgaaacattt ttgtatcaat taattgctga tgccgcacct
     1801 attcttttac tggtgctaga tggcgttacc gatccgcata atttaggggc ctgtctgcgc
     1861 tcagccgatg ctgcgggtgt tcaagcggtt attattccca aagacaattc agcgggttta
     1921 accgagactg cgcgcaaagt tgcctgtggt gcggccgata gtgtgccctt ggtgagcgtg
     1981 acaaacttat cccgctgttt agaaaaatta caggagttgg gcgtgtggtt agttggcacc
     2041 acgggagatg ccaaagatac catttatgat cttgatctca ccggcaatat agcggtgata
     2101 atgggagctg aaggcagtgg tatgcggcgt ttaacccgcg agcactgcga ttatctagcg
     2161 catattccca tgtgtggcag cgtcagtagt cttaatgtat ctgtagctac cggcgtgtgt
     2221 ttatttgagg ctctgcgcca gcgcagacaa tcctaagccg attatccggg ttaataatga
     2281 gaagcggagt cagtaaacat gcggttatgt gtatttagca gtaaacccta tgatgagcag
     2341 tatctgcgtc ccgccttaga aggcgaatgt gaattagttt ttttgccggt gcacctcaat
     2401 aaaaatactg tgcccttagc tgagggggca gggggtattt gttgtttcgt caacgatgac
     2461 ttatcagccc cggtccttga agctttggct ctgcgcggta tccaatttat tgtgttgcgc
     2521 tgtgcagggt ttaatcaggt ggatgtggca gcggcaaaac gcctaaaatt ggtaattgcc
     2581 cgagtgccgg aatattcacc ctatgcagtg gctgaacatg ccgttgcact tattttggcg
     2641 cttaatcgca atattcaccg ggcgcacaat cgcgtgcggg aaggtgatta ttcgctacag
     2701 ggtttaatgg ggtttgattt acacggtaaa accgtaggga ttgtcggcgc cggtaaaatt
     2761 ggccgcgcat tcgctgcaat tgtgcgtggt tttggttgca cggtgttaat ctgcgatcct
     2821 gtggccgacc ctgccagctg cgattgggcg cgctgggttg attttgattc tttaatgcag
     2881 cagtctcata tagtgtccct gcattgtccg ctcacaccgc acactcacca tttaattaat
     2941 gctcgcgcat tggctttaat gcagcctggg gccatgttaa tcaataccag tcgtggcgct
     3001 ttggtggatg cgcgggccgt tatagatgct ttgaaatccg gccatttggg ttatttaggt
     3061 ttggatgtgt atgaagagga gggggatcta ttttttgagg atttatccaa tcaggttatt
     3121 caagacgatg tatttgcccg cttgaccacc ttccctaatg ttttaattac tgcgcaccag
     3181 gcttttttta cccacgaagc gctgacccgg attgccgagg tgactttgtt aaatctgcgc
     3241 gccattaagc agggtgcgct tatacctgcc aatcttgtcg c
//
LOCUS       DAOQGS010000468         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17041_length_3277_cov_4.769088, whole genome shotgun sequence.
ACCESSION   DAOQGS010000468 DAOQGS010000000
VERSION     DAOQGS010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17041_length_3277_cov_4.769088"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKZ52_14810"
     CDS             complement(<1..249)
                     /locus_tag="PKZ52_14810"
                     /inference="COORDINATES: protein motif:HMM:NF015608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNG61093.1"
                     /translation="MLWLKAFHIVAFTSWFAMLFYLPRLFVYHQMADDAASKERFSLM
                     EHKLYKVIGTPAMLVTLLLGISMAGMGWDYYKTQGWFHA"
     gene            complement(320..1645)
                     /locus_tag="PKZ52_14815"
     CDS             complement(320..1645)
                     /locus_tag="PKZ52_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNG61094.1"
                     /translation="MVKAFLVKLKLALTGPGHLGLMCGYALAVGLAVSLCVLLFRAAI
                     DGLCARLLGIKPDYFEQLSFLSQGISLAAGSAVLWVLYRWAGPQSQQLSVAHVIDRVH
                     NHQGHMPLRNWALQWAGAVASLVCGQSVGQEGPIVHLGAGVGAWLGKRRALAPEECRT
                     LLAAGVAAAIGACFDTPLAGVILAFEVVLLRWQMDGVLPVMLAAVVGALAYQATALPS
                     LQVRFSDLPALTFGGSLGWAALGAPLLGLAAGLYVLANRWGLRLRQWPLSLRLLLAGL
                     STWLVAAWQPAVLGLGFDSLTAVLNHQLPLAALVTLGLCKLVLSPWVVGLGVPGGLIG
                     PNLLAGACLGGAWGVLGVTVLGLPPEAAKLFALLGMAAVMAASLNAPLAALVAVLELA
                     RSADIILPAMVMIALACLVQQQLLGFKGLFVEQLQQAGKPLPAAGASPP"
     gene            1698..2738
                     /gene="argC"
                     /locus_tag="PKZ52_14820"
     CDS             1698..2738
                     /gene="argC"
                     /locus_tag="PKZ52_14820"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNG61095.1"
                     /translation="MVRIGIVGGTGYTGVELLRLLAAHPKAQVEVITSRAEEGMPVAD
                     MFPNLRGHYDLRFSAPDFNQLNELDVVFFATPHGVAQAMMPKFMDSNTRVIDLSADFR
                     IRDIALWERWYGQPHGCPELVPQAVYGLPEVNRDKIRTAKLIACPGCYPTSVQLGFLP
                     LIERGLIDTSRLIANSASGVSGAGKQAKVDNLFAELNDSFKAYGVAGHRHLPEIEQGL
                     NDLQAAGAPGVNLTFVPHLLPTVRGIHSTLYATLTQAGTDLQALFEERYKDEPFVDVL
                     PQGQYPQTRTVRGTNLCRIAVAQPQGRDTVVVMVAEDNLTKGASGQAIQNMNIMLGLP
                     ETMGLNQPALLP"
     gene            2747..>3277
                     /locus_tag="PKZ52_14825"
     CDS             2747..>3277
                     /locus_tag="PKZ52_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61096.1"
                     /translation="MSTVKVKGSKHYPMKVVPHRPLRDWAIRAGIALAFVALVAGSYL
                     QRQWHSSQLVEDLAQAQAALKQAQSAEAQARQQLANMEMGAEVDRKSSQDVRAEISAL
                     KEQVQLQQQQLSFYRSLMNPAEGKQGLAIGELEYTRGAEPQRYHYSLKVLQLANLDQI
                     SDFEGDWHFKLVGKQQG"
BASE COUNT          704 a         1063 c          961 g          549 t
ORIGIN      
        1 ggcgtgaaac cagccttggg ttttgtaata gtcccagccc atgcccgcca tgctgatgcc
       61 cagcagcagg gtaaccagca tagccggggt gccgatgacc ttgtacagct tgtgctccat
      121 caggctaaag cgctccttgg aggccgcatc atcggccatc tggtggtaca caaacaggcg
      181 cggcaaatag aacaacatgg caaaccaact ggtaaaggcc acaatgtgaa acgcttttaa
      241 ccacaacata gggattcctc gagcaaaggg ccgtggactg tatctttaac ctctgcccag
      301 cgcaatctga gggggaggat cagggtgggc tcgcccctgc tgctggcagg ggtttacccg
      361 cctgttgcaa ctgctccaca aataaaccct taaaccccag cagttgctgc tgcaccaggc
      421 aggccagggc gatcatcacc atggcgggca gaataatatc ggcgctgcgg gcgagttcta
      481 acaccgccac cagggccgcc agaggtgcat ttaaactcgc tgccatcact gccgccatgc
      541 ctaacagggc gaatagcttg gctgcttccg gcggcaggcc caagaccgta acccctaaca
      601 caccccaggc gccccccaaa caggcccccg ccaagaggtt cgggccgatc aagccgccgg
      661 gcaccccaag ccccaccacc cagggggaga gcaccagttt gcacaggcct agtgttacta
      721 gggctgcgag tggcagttgg tggttgagca ccgccgtcag gctgtcaaag cccaaaccca
      781 gcacggcagg ttgccaggcg gccaccagcc aggtgctcaa accggccagc agcaggcgca
      841 ggctgagggg ccattggcgc aggcgcaggc cccagcggtt ggcaaggaca tagaggccag
      901 cggcaagccc aagcagcggc gcccccaggg cggcccagcc taaagagccg ccgaatgtaa
      961 gtgcgggcag gtcgctaaag cgcacctgca ggctcggcag ggcggtggcc tgataggcga
     1021 gcgcccccac caccgccgcc aacatcaccg gcaacacgcc gtccatctgc cagcgcagca
     1081 gcactacttc aaaggccaga atcactccgg ctaagggggt atcgaaacag gcgcctatgg
     1141 ctgcggccac cccggcggcg agcagggtgc ggcactcctc gggggccagc gcgcgccgct
     1201 tgccaagcca agcgcccacc cccgccccca ggtgcactat gggcccctct tggcccacgc
     1261 tctggccgca taccaagctt gccacggcgc cggcccactg cagcgcccag ttgcgcaggg
     1321 gcatatggcc ctggtggttg tgcacccggt cgatcacatg ggccacgctc agctgctggc
     1381 tttgcggccc cgcccagcga tacaacaccc acagcactgc acttcctgcc gcaaggctta
     1441 tgccctgact taagaagctg agctgttcaa aataatccgg cttgatccca agcagtcggg
     1501 cgcacagccc gtcgatggcg gcgcgaaaca gcagtacaca caggctcaca gcaaggccca
     1561 ccgccagggc atagccgcac atcaggccca ggtggccggg ccctgtgagg gccaacttta
     1621 gttttactag aaaggccttt accatacgct cctctttttc ctacacccct gcaccagcgg
     1681 ggtgcgacga ggttgctatg gttcgaattg gtattgtggg tggcaccggc tacaccgggg
     1741 tggagttgct gcgcttgctg gcggcccacc ctaaggcaca ggtggaggtg atcacgtcgc
     1801 gggcggaaga gggcatgccg gtggcggata tgttccccaa tctgcgcggc cactacgacc
     1861 tgcgctttag cgccccggat tttaaccagc ttaatgaatt ggatgtggtg tttttcgcca
     1921 ccccccacgg cgttgcccag gccatgatgc ccaaattcat ggactccaac acccgcgtga
     1981 tcgacctctc cgctgacttt cgcatccgcg atatagccct ctgggagcgc tggtacggcc
     2041 agccccacgg ctgccccgag ctggtacccc aggccgtgta tgggctgccc gaggtcaacc
     2101 gagacaaaat cagaaccgcc aagctcatcg cctgccccgg ctgttaccct acctctgtac
     2161 agctgggctt tttgcccctg atcgagcggg gccttatcga caccagccgc ttaatcgcaa
     2221 acagcgccag cggtgtcagc ggcgcgggca agcaggcgaa ggtggataac ctcttcgccg
     2281 agctgaacga cagtttcaaa gcctatgggg tggcgggcca ccgccatctg ccggagatag
     2341 agcagggcct taacgacttg caagcggcgg gagcgccggg ggttaacctc acctttgtgc
     2401 cgcatttgtt acccaccgtg cggggcatcc actccaccct gtacgccact cttacgcaag
     2461 cgggtactga cctgcaggcc ctgtttgaag agcgctataa agatgagcct tttgtggatg
     2521 tattgcccca aggccagtac ccgcagaccc gcaccgtgcg cggcaccaac ctgtgccgta
     2581 tcgccgtagc ccaaccccag gggcgcgaca cagtggtggt gatggtggcg gaggataacc
     2641 tcaccaaagg cgcctccggc caagcgattc aaaacatgaa cattatgctg ggcctgccgg
     2701 aaaccatggg ccttaaccaa cccgccctct tgccctaggc ccctctatga gcacggtaaa
     2761 agtcaaaggc agtaaacact accccatgaa agttgtgccc caccgccccc tgcgggactg
     2821 ggctatccgc gcgggcatag ctctggcttt tgtggcactg gtggcaggca gctatttgca
     2881 gcgccaatgg catagcagcc agctggttga ggatttggcc caagcccagg cggcgcttaa
     2941 acaggcccag agcgccgagg ctcaagcccg ccagcaactg gccaatatgg agatgggcgc
     3001 cgaggtggat cgcaagtcca gccaagatgt gcgcgcggaa atcagcgcac tgaaagaaca
     3061 ggtgcaatta caacagcagc aattgagttt ttaccgctcc ctaatgaacc cggcagaggg
     3121 caaacagggc ctggccatag gtgagctgga atacacccgc ggcgccgagc cccagcgtta
     3181 tcactacagc cttaaggtgt tacagctggc taacctggat caaatcagcg attttgaagg
     3241 cgactggcac tttaagctgg tgggcaaaca acagggc
//
LOCUS       DAOQGS010000469         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17113_length_3270_cov_4.076205, whole genome shotgun sequence.
ACCESSION   DAOQGS010000469 DAOQGS010000000
VERSION     DAOQGS010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17113_length_3270_cov_4.076205"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..519)
                     /locus_tag="PKZ52_14830"
     CDS             complement(16..519)
                     /locus_tag="PKZ52_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNG61097.1"
                     /translation="MLRALVFFLVCLSTATGWAAIDTFEFSSEENRERYSILVDELRC
                     PKCKNNNLSGSNSMIAMDLKREIHSMVEEGKSNQQIADFMVARYGDFVLYRPRMTTQT
                     ALLWGAPVGLFLVALIVVISLVRGRARALKVEPGTALSPEEQAQLLRLTGAASSAQSN
                     HSKPHQG"
     gene            715..790
                     /locus_tag="PKZ52_14835"
     tRNA            715..790
                     /locus_tag="PKZ52_14835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Val,seq:tac)
     gene            833..909
                     /locus_tag="PKZ52_14840"
     tRNA            833..909
                     /locus_tag="PKZ52_14840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Asp,seq:gtc)
     gene            1173..2630
                     /locus_tag="PKZ52_14845"
     CDS             1173..2630
                     /locus_tag="PKZ52_14845"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG61098.1"
                     /translation="MAVTGVEAWVKKLALQSMPVASEVVAELNKLTGSDDATLQQMGE
                     AILRDPHLTSHVLRVANSVVHNQGRAPINTVSRAIALLGVKGMRAISISVLFIDSLLA
                     KGPKNHLLQLMAKGFHAANQAKNLVMRFDEQAGEEVFVAALLHHVGEMAFWSCEKLTQ
                     ERQQLLNADATKRENFAKAYLGTGFKEISLGLARHWKLGSTLVESLEAGAEENLSRKV
                     QVILLAERISQAAPGGWESPPMQAIVREIAEITQVDEQVALTWILDGAEQAAEIALKY
                     GAHELCPFIPGRDHRLDASRPMSVGRVLQADPQVQLNILRELGAANAEALDVNTIFKM
                     VIEGMHRGIGLERVVLAFIQHHRVSAKFVLGQGTEDWKQTFNFDASPFCDNMFTHAIK
                     TGGAIWFKGDELKRLCATTDSQPIVDCLGEAECLVFALDVNGRKPAVFYADRGSFGGA
                     IAQDHFESFRHFALQAQTNLCRVSAPKPRPVSAAR"
     gene            complement(2632..>3270)
                     /locus_tag="PKZ52_14850"
     CDS             complement(2632..>3270)
                     /locus_tag="PKZ52_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNG61099.1"
                     /translation="ALAAYNCGELNVTNAVARNKAKGLPTDFWSLPLPAHTKTYIPKI
                     MAIREIITTPAKYKVDLHPIPNRPYFAKVNVASAISLSAAANVTGVDVKDLQALNPAL
                     TRAATDPVITKSLLVPASSAQTFASTINNMPDKSQLAPKNEPLVQVASAAKPAKAVAE
                     QGGKITHELKKGESLWTLSKRYKVSMDQIAQWNNLKPKQDMKPGKQLIIWQR"
BASE COUNT          686 a          834 c          975 g          775 t
ORIGIN      
        1 gccatcgtca tagagttacc cttggtgtgg tttagagtgg ttggattgag cgctggaggc
       61 agcgccggtg aggcgcagca actgggcctg ctcttcaggg cttaaggcgg tgccaggctc
      121 caccttaagc gcccgtgcgc gcccgcgcac caggctgatg accacaatca gcgccactaa
      181 aaacaggccg acgggggcgc cccacagcag cgcggtttgg gtggtcatgc gggggcggta
      241 gagtacaaaa tcgccgtagc gagccaccat aaaatcggcg atttgttggt tgcttttgcc
      301 ctcctccacc atgctgtgaa tctcccgttt taggtccatc gcaatcatgg agttggagcc
      361 ggagagattg ttgtttttgc atttggggca gcgcagctca tccaccagaa tcgaatagcg
      421 ctcacgattt tcctcgctgc taaattcaaa ggtatcaatg gcggcccatc cagtggcggt
      481 agacaaacaa accaggaaaa aaactaaggc tcgaagcata gggggtgcct tggcgttaag
      541 gggcaaaatg ggcccggatt atagcagtga ggacttgcaa cagtgggaat tgcgttgcga
      601 tttccacccc ccgcttggat acccgagaac aaataaattc ctttgatttc aataattttt
      661 attgacgagc gagtgtaagt cgttagaatg cgcggctcct aacacaggca ctgcgggtgg
      721 ttagctcagc tgggagagcg gcagccttac aagctgtagg tcacaggttc gatccctgta
      781 ccacccacca ttaatacgag ccttgctctt gatggcgtta agccactacc gcggaccggt
      841 agttcagttg gttagaatgc cggcctgtca cgccggaggt cgcgggttcg agccccgtcc
      901 ggtccgccac ttattgaaaa gccctgcact gcagggcttt tttttcgccc gctatttccg
      961 catttattac ccaaaccggc tccagctaat accttcatcg actggaaact atggttttgc
     1021 cgccatgggc aaaatgttgt cggtaaaagc ccctagtttg tgtgtactgg cctatgctta
     1081 tttagatcaa ccaaggatag cgcgcccggt tagctgccaa gataggttct aaccggcctg
     1141 cgctattaac gcgtgagttt ttagggggtg ccatggcggt aacaggggta gaggcctggg
     1201 ttaaaaagtt ggctttgcaa tccatgcctg tagcgagcga ggtggtggcg gagctcaata
     1261 aattaaccgg ctctgacgat gccacgctgc agcaaatggg cgaggccatc ctgcgggacc
     1321 cgcaccttac ctcccatgtg ttacgggttg ccaacagcgt ggtgcacaac caaggccgtg
     1381 cgccgattaa taccgtaagc cgcgccattg ccctgctggg ggtgaaaggc atgcgggcta
     1441 tctccatctc ggtactcttt attgattccc tcctggccaa aggtcctaaa aatcatcttt
     1501 tgcaattgat ggcaaaaggc tttcacgccg cgaatcaggc gaaaaacctt gtgatgcgat
     1561 ttgatgaaca ggccggggag gaagtgtttg tggcggcgct gctgcatcat gtgggcgaga
     1621 tggcattctg gtcctgcgaa aaactcaccc aagagcgcca gcaactttta aatgccgatg
     1681 caaccaagcg cgagaacttt gccaaggcct atttgggcac cgggtttaaa gaaatttccc
     1741 tgggcctggc ccggcattgg aagctcggtt caaccctggt ggagtccttg gaggcggggg
     1801 ctgaggaaaa tctctcgcgc aaggtgcaag tcattttatt ggccgaacgc attagccaag
     1861 cggcgccggg cggttgggag agcccaccga tgcaggcgat tgtgcgcgag atcgccgaga
     1921 tcacgcaggt ggatgagcag gtggcgttga cctggatctt ggatggcgcc gaacaggcgg
     1981 ctgaaatcgc ccttaaatac ggcgcccatg agctttgccc gtttattcct gggcgcgatc
     2041 atcgtttgga tgcatcccgc cctatgtcgg tgggccgggt gctgcaggcg gacccgcagg
     2101 tgcagttaaa cattctgcgt gagttgggag cggccaatgc cgaagccttg gatgtgaaca
     2161 ccatttttaa aatggttatc gaaggtatgc accggggcat cggcctggag cgggtggtgc
     2221 tagcgtttat tcagcaccac cgcgtgtcgg ctaagtttgt attggggcag gggacggaag
     2281 actggaaaca gacgtttaat tttgacgcca gccctttctg cgacaacatg tttacccatg
     2341 caattaaaac cggcggtgcc atatggttta aaggcgatga gttaaaacgc ctgtgcgcca
     2401 ctaccgactc ccagccgatt gtcgattgct taggggaggc ggagtgtttg gtgtttgcgc
     2461 tggacgtcaa cggccgcaaa ccggctgtgt tctacgccga ccgaggcagt ttcggcggcg
     2521 ctattgccca ggatcatttc gagagtttta ggcactttgc cctgcaggca caaactaatt
     2581 tatgccgcgt ttccgccccc aagccgcggc cggttagcgc cgctagataa gttagcgctg
     2641 ccagataatc aactgtttac ccggcttcat atcctgcttg ggctttaggt tgttccactg
     2701 ggcaatttga tccatggaca ccttatagcg tttgctcaaa gtccacagag actcgccctt
     2761 tttcagctcg tgggtaattt taccgccttg ctccgctacg gccttggcag gtttggctgc
     2821 ggaagccacc tgcacgagcg gctcattttt cggcgccaac tggcttttat ccggcatatt
     2881 gttaatggtg ctggcgaagg tctgcgccga cgaggcgggc accagtaggc ttttagtgat
     2941 cacaggatct gtcgccgccc gggtaagggc cgggttgagt gcttgtaagt cttttacatc
     3001 gacgcctgtg acattggcgg cggccgacaa gctgatagct gaagccacat tgaccttggc
     3061 gaagtagggg cggttgggga tggggtgcag gtcaactttg tatttggctg gggtggtgat
     3121 gatctcccgg atcgccatga tcttggggat ataggttttg gtatgagccg gtagcggcag
     3181 ggaccaaaaa tctgtgggga ggcccttcgc cttgttgcgt gctacggcgt tggtcacgtt
     3241 caactcaccg cagttatagg ctgctagagc
//
LOCUS       DAOQGS010000470         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17223_length_3254_cov_3.776180, whole genome shotgun sequence.
ACCESSION   DAOQGS010000470 DAOQGS010000000
VERSION     DAOQGS010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17223_length_3254_cov_3.776180"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PKZ52_14855"
     CDS             <1..1407
                     /locus_tag="PKZ52_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61100.1"
                     /translation="LQLSRAQGTSSHADKRLTSAPMAPFARIERKLQLGLEWSVETTI
                     TRIAPATGPIHLDVPLLAGEAPLGVQPNAQGLMPVNLANDEDSFSWSSRLKIAPELVL
                     VAPEQPAWVEDWQLEAASQWHIQAEGVPQLGNNLAPRWQPWPAEQLRIQVGKPPAVKG
                     NNLTVQAVSLTQRVGQKAQDVTLELNILSNQGQDFILSLPAEAKLNSIKRDGGELPPI
                     VQDGRVKLPVKPGNQQFSVQWQQLEELPLRLHTAQLDLGLPARNINLNLQLPADRWIL
                     LLGGPQMGPALLFWGVLCVVLGLAWALGRSGLAHLKSYEWVLLSLGVATQSLSVLVIL
                     AAWFAALRWRGQQSGWTGGRYKTLQVGLVGLSLLALGCLLGAIPQGLLSSPDMQIHPA
                     SYGGLSWYSDYSAGLMPQAWVISLPMWVYRVSMLLWSLWVAFALMGWLKWGWQQLNAG
                     GFWPGDKPDAAPAQAPNL"
     gene            1456..1791
                     /locus_tag="PKZ52_14860"
     CDS             1456..1791
                     /locus_tag="PKZ52_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HNG61101.1"
                     /translation="MNDQLLNLINQPASQFSAVIAWIDQHYSFTPTQFQNGAQLNEAN
                     QNNGSCKVFALAQLHSLTAEQTLQLFCEHYRAVLEHPEGTDHQNIRQFMAQGFGGLRF
                     SGEALALRS"
     gene            1877..>3254
                     /gene="mnmG"
                     /locus_tag="PKZ52_14865"
     CDS             1877..>3254
                     /gene="mnmG"
                     /locus_tag="PKZ52_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNG61102.1"
                     /translation="MIYPTSFDVIVIGGGHAGTEACLAAARMGCKTLLLTHNIETLGQ
                     MSCNPAIGGIGKSHLVREIDALGGAMARATDRAGIQFRVLNLRKGPAVRATRAQADRI
                     LYKAAIRHILENQPNLSIFQQAVDDLIVEGDTVKGVITQMGVKFLAPSVVLTAGTFLG
                     GKIHIGLENHSGGRAGDPPSIALAQRLRELPLRVDRLKTGTPPRIDARSVNFAGLEEQ
                     WGDTPTPVMSFMGSVAEHPRQTCCWITHTSAKTHDIIRSGFDRSPMFTGVIEGVGPRY
                     CPSIEDKVNRYGDKDSHQIFIEPEGLTTHELYPNGISTSLPFDIQLALVRSIKGFENA
                     HILRPGYAIEYDFFNPQDLKYSFETRALNGLFFAGQINGTTGYEEAGAQGLLAGLNAA
                     LRAQEKEAWCPERDQAYIGVLADDLITLGTKEPYRMFTSRAEFRLMLREDNADARLTE
                     KGRELGLVS"
BASE COUNT          654 a         1024 c          928 g          648 t
ORIGIN      
        1 ctgcagctca gccgcgccca aggcaccagc agccatgccg ataaacgcct cacgtcagcc
       61 cccatggcgc cttttgcgcg catcgaacgc aaactgcaat taggcctgga atggagcgtg
      121 gaaaccacca tcacccgcat agcgccagcc accggcccca ttcatttgga tgtgccgctg
      181 ttggccgggg aggcgccctt gggtgtgcag cccaatgccc agggcttgat gccggtgaat
      241 ctggccaatg atgaagacag tttcagctgg agctcccgcc tgaaaatcgc cccggagctt
      301 gtgctcgtgg ccccggagca gcccgcctgg gtggaagact ggcaactaga ggccgcctcc
      361 caatggcata ttcaagctga gggtgtgcct cagttgggca ataacctggc gcctcgctgg
      421 cagccctggc ccgctgagca actgcgcatt caagtcggta agcccccggc ggtgaagggc
      481 aacaacctca ctgtgcaggc ggtttccctt acccagcggg tggggcaaaa ggctcaggac
      541 gtcaccctgg aactgaatat cctcagcaac cagggccagg actttatcct cagcctaccg
      601 gccgaggcca agctcaacag tatcaaacgc gatggcggcg agttgccacc catagtgcag
      661 gacggtcgcg tgaagctgcc cgtcaaaccg ggcaaccagc agttttcggt gcagtggcag
      721 cagttggagg aattgcccct gcgcctgcac accgctcaac tggacttggg cctgcccgcg
      781 cgcaatatca acctgaattt gcaactgccc gccgatcgct ggattttgct gctcggcggc
      841 ccccaaatgg gcccggccct gttgttttgg ggggtgctct gcgtggtgtt gggcctggcc
      901 tgggcgctgg gcagaagcgg ccttgcccac ctgaagagtt acgaatgggt attgttaagc
      961 ctgggggtgg ccacccagag cttgagtgtg ttggtgatcc tcgccgcctg gtttgccgcc
     1021 ttgcgctggc gcggccagca gtccggctgg accggcggtc gctacaaaac cctgcaagtg
     1081 ggcctggtgg gcctatccct gctggccttg ggctgcctgc tgggcgccat tccccagggc
     1141 ttgctctcaa gcccggatat gcaaattcac ccagccagct acggtggcct cagttggtac
     1201 agcgactaca gcgcaggcct tatgccccaa gcctgggtga tttccctgcc catgtgggtg
     1261 taccgggtga gcatgctgct gtggtccctc tgggtcgcct tcgccctaat gggttggtta
     1321 aagtggggct ggcagcagct caatgctggc ggcttttggc ctggagataa acccgatgct
     1381 gcgcccgctc aggcacctaa cctctagttc ccagccctcg cactttggtg cgagggcatt
     1441 caattgagac tccctatgaa cgatcaactg ctcaacctaa ttaaccaacc ggccagccaa
     1501 ttcagcgctg tcatcgcctg gatcgaccag cactacagct ttacccccac gcaatttcaa
     1561 aacggcgctc aactcaatga ggccaatcaa aacaacggct cctgcaaagt cttcgccctg
     1621 gcccagttac acagcctcac cgccgagcaa accctgcaat tgttctgcga gcactaccgc
     1681 gccgtgcttg agcacccaga gggcacggat catcaaaaca tccgccagtt tatggcccag
     1741 gggtttggcg gtttaaggtt tagcggggag gcattagcgc tgcgcagtta aatactggaa
     1801 ttgtgcgttt aatttttgta ccgctggggc aaactctcta taatctgcgc cccttcgctt
     1861 aagactgccc tctgccatga tttaccccac ttcgtttgat gtcatcgtca ttggcggcgg
     1921 ccatgcgggt acggaagcgt gcctggcggc tgcccgtatg ggctgcaaaa ccctgctgct
     1981 cacccacaat atcgaaaccc tcggccagat gagctgcaac cccgccatag gcggtattgg
     2041 caaaagccac ctggtgcgcg agatagacgc tttgggcggc gccatggcgc gggcgacgga
     2101 ccgagcgggc attcaatttc gcgtgttgaa cctgcgcaaa ggccccgccg tgcgcgccac
     2161 ccgcgcccag gcggaccgca tactttacaa agcggccatc cgccatattt tggaaaacca
     2221 gccgaacctg agtatcttcc agcaggcggt ggacgattta attgtggaag gggacacggt
     2281 caaaggtgtt atcacccaga tgggggtgaa attcttagcc cccagcgtag tgctcaccgc
     2341 aggcaccttt ttgggcggca aaattcatat tggcctggaa aaccactccg gcggccgggc
     2401 aggggaccca ccctccatcg ccctcgccca gcgcttgcgc gaattaccct tgcgcgttga
     2461 ccgcttaaaa accggcaccc cgccgcgcat tgacgcccgc agcgtgaact ttgctggcct
     2521 ggaagagcag tggggcgata cccccactcc ggtgatgagt tttatgggct cggtggccga
     2581 gcacccgcgc caaacctgct gctggatcac ccataccagc gccaaaaccc acgacatcat
     2641 ccgctcaggc tttgatcgct cgcccatgtt taccggcgtg atcgaagggg ttggcccccg
     2701 ctattgcccg tccattgagg acaaggtcaa ccgctatggg gataaagact ctcaccagat
     2761 ttttatcgag cctgaaggcc ttaccaccca cgagttgtac cccaacggca tctccaccag
     2821 cctgccgttt gatatccaat tggccctggt gcgctccatt aagggctttg aaaacgccca
     2881 tatcctgcgc ccgggttacg ccatcgagta cgactttttt aacccgcagg atcttaaata
     2941 cagctttgaa acccgcgccc tcaacggcct attcttcgca ggtcaaatca acggcaccac
     3001 cggttatgaa gaggcggggg cccaggggtt actcgccggt ctaaacgccg cgctgcgcgc
     3061 ccaggagaaa gaggcctggt gcccagagcg ggaccaagcg tatataggtg tgttggccga
     3121 tgacctcatc accctgggca ccaaagaacc ctaccgcatg tttaccagcc gggcggaatt
     3181 tcgcttgatg ctgcgcgaag ataacgccga tgcgcgcctc accgaaaagg gccgcgagtt
     3241 aggcctggtg agtg
//
LOCUS       DAOQGS010000471         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17245_length_3252_cov_4.610572, whole genome shotgun sequence.
ACCESSION   DAOQGS010000471 DAOQGS010000000
VERSION     DAOQGS010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17245_length_3252_cov_4.610572"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3252)
                     /locus_tag="PKZ52_14870"
     CDS             complement(<1..>3252)
                     /locus_tag="PKZ52_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG61103.1"
                     /translation="PTPQSLENTKGIITALYEDNKKRLWVGVQGGGLYIRAEHQNEFT
                     VYTPPVALQFDLATATVRDVKQDKEGFLWMATDNGLVKIDETRKTAVWYQHEDNNPHS
                     LGDNRLWKIMLDKDQNLWISSDHGGVAVYIRSADRFDRYEHDPYNADSVNSNQVRQIY
                     QDRLGDYWVGLFPVGVDYINAEVANFTIFRHQPDQPNGLPNDALLSMYPAAGNSVWIG
                     SEAGLTLFDTVKKEFTNFAHDTNNPKSLSANAVISMTKDMYGNYWFGTWSGGLNRFNE
                     KDKTFERFVPQADNPNSVSSSYIWGLMGDKDGNLWIGTEAGSLDKYAINTGKFTHYVP
                     NESDPKALSGHYIRAIVQDSDGDIWVGTLNGLNKFNPQDESFTRYQHDDAKPNSLPHN
                     SVGFIFEDSHRNLWVATENGVALFDKKSGTFTNYSVAQGLAHESVTAIAEDAEGDIWF
                     TTMNGLSRFNPKTQKFRNFSKANGLAGNIMSKPVIYAAPGGLLLVGSTQGLAMFNPAN
                     LLENTLVPPVFLTGFKIFNKSVPISADSEVLTRHIHGANRINLNYHQTMFSLDFSALN
                     FRNASDNQYKFKMEGFDQDWIDAGKLRSATYTNLNPGDYRFLVMGSNNNGVWNQLGAS
                     VDIHVAPPPWRTIYAYCFYVLVVLGIIYIFVRSQRLKVQFEQEKVLQLQTINKLKDEF
                     LANTSHELRTPLNGIIGIAEALVDDNAEELGPRVLSQLQMIAQSGRRLSNLVNDILDF
                     SRMRHKGFDLRLRSINVVNLANSVVSMTLPLARKKNLQVFCELNDNLPRVAADEERLE
                     QILYNLIGNAIKFTLQGHVRISAHEENNSVYITVSDTGIGIPESDLVSIFDSFSQVKG
                     DATREFEGTGLGLAVTKNLVELHGGTITVKSTLGKGSEFTFNLPIAESDLEQDEQRPN
                     DTRLRSVIEAAAPVVPVELETVEESKDPNGFKPHILIVDDDTINRQVLISQLGAAQYR
                     FTEAVNGEDAINAVMQDFSIDLVLLDVMMPKMTGYDTAQRIRKIRQRQDLPIIFITAK
                     HFATDLLTGFGVGGNDFLTKPVSKPELLSRTKTHLDLSRITRNLEKIVEERTNT"
BASE COUNT          847 a          755 c          767 g          883 t
ORIGIN      
        1 ggtattagtg cgctcctcaa caattttttc caggttgcgc gtaatgcgcg ataagtccaa
       61 atgagttttg gtgcgtgata ataactcggg tttagagact ggcttagtta aaaagtcgtt
      121 gccgccaacg ccaaaaccgg ttaatagatc cgtcgcgaaa tgtttggcgg tgataaatat
      181 gattggcaaa tcctggcgct ggcggatttt gcggattcgc tgggctgtgt cgtaaccggt
      241 cattttgggc atcattacat ccagcaatac aagatcaata gaaaaatctt gcataacggc
      301 attaatagca tcttcgccgt ttacagcttc ggtaaagcga tactgagccg ctcccaattg
      361 gctaattaat acctgccggt taatggtgtc gtcatccaca attaaaatat gcggtttgaa
      421 gccgttgggg tcttttgact cttcaaccgt ttccaattca acgggaacta ctggtgcagc
      481 cgcctcaata acagagcgca aacgggtgtc gttggggcgc tgctcgtctt gttctaaatc
      541 actttcagcg ataggcaaat taaaggtaaa ttcagagcct ttgcccaggg ttgatttaac
      601 cgtgatggta ccgccgtgta attccactaa atttttagta accgcaaggc caaggccggt
      661 gccttcaaat tcgcgggtgg catcgccttt tacttgggaa aatgagtcga atatgctgac
      721 caaatcagat tcagggatac cgattcccgt atcggatacc gtgatataaa cactattatt
      781 ttcttcatga gcggaaatac gcacgtggcc ctgcagggta aatttaattg cattgccaat
      841 taaattgtag agaatttgct ccaagcgctc ttcatcggca gcaacccgcg gcaaattatc
      901 attcagctcg caaaatactt gcagattctt tttgcgcgcc aagggcagcg tcatactcac
      961 aacagaatta gcaaggttaa ccacattaat tgagcgcagg cgcagatcaa agcccttatg
     1021 gcgcatacgg gagaaatcga gaatatcatt gaccaggttg gacagccggc gaccactttg
     1081 cgcgatcatc tgcagctgtg ataagactct tgggcctagc tcctctgcat tgtcatcgac
     1141 taaggcctca gcaataccaa taataccgtt taggggcgta cgcagttcat gggaggtatt
     1201 ggccaaaaac tcgtctttta atttattgat ggtttgcaat tgcaaaacct tttcctgctc
     1261 aaactgtact tttagacgct gtgaacgcac gaaaatataa ataataccca gcacgaccaa
     1321 tacgtaaaaa caataggcat aaatagtgcg ccagggcggc ggtgctacgt gaatgtccac
     1381 cgaggcaccc agctgattcc ataccccgtt gttgttagaa cccatcacca aaaagcgata
     1441 gtcgccgggg tttaggttgg tgtaagtggc gctgcgtaat ttgcctgcat caatccaatc
     1501 ctgatcaaag ccttccattt taaatttata ttggttatcg ctggcattgc ggaaatttag
     1561 ggcggaaaag tccagcgaga acatggtctg gtggtaattt aaattgatgc gattagcgcc
     1621 gtggatatgg cgggttaaca cttcgctatc ggcagagatg ggcacacttt tgttaaagat
     1681 tttaaagccc gttaaaaaca ccggcggcac cagggtgttt tccaataagt tggcggggtt
     1741 aaacatggcc aagccttgag ttgaacccac caataacagg ccaccgggtg cggcataaat
     1801 aacaggctta ctcatgatat tgccagccag cccattggct ttggagaaat tgcgaaactt
     1861 ctgcgttttg gggttaaacc ggctgaggcc attcatggtg gtaaaccaaa tatcgccctc
     1921 ggcgtcttcg gcaatcgccg taaccgattc atgggccagg ccttgagcaa cggaataatt
     1981 ggtaaaggtg ccagattttt tatcaaataa ggcaacacca ttttcagtgg ctacccacag
     2041 attgcggtgt gagtcctcaa aaataaatcc aaccgaatta tgcggcaggc tgttgggttt
     2101 agcgtcgtcg tgttgatagc gggtaaagct ttcatcctgt ggattaaatt tatttaagcc
     2161 attgagggtg cccacccata tatcgccgtc gctgtcttgc actatggcgc gaatatagtg
     2221 gccgcttaag gccttggggt cggattcatt gggtacatag tgagtgaatt tgccagtatt
     2281 aatcgcatat ttatccaggc tgcccgcttc agtgccaatc cacaaattgc catctttatc
     2341 ccccatcaag ccccatatgt aactggagct taccgaatta gggttatccg cctgcggcac
     2401 aaaacgctca aaggttttat ctttttcatt aaaacgattt aagccccctg accaggtgcc
     2461 aaaccaataa ttgccgtaca tatctttggt catggatata acagcattgg cgctgaggct
     2521 tttggggtta ttggtgtcgt gggcgaaatt ggtgaattct tttttcaccg tatcaaacag
     2581 tgttaagccc gcctctgaac cgatccagac gctattgcct gcggctgggt acatactgag
     2641 taatgcgtca ttgggcaagc cattgggctg atcgggttgg tggcgaaaaa tagtaaaatt
     2701 ggccacttcc gcattgatat aatccacgcc cacggggaat aggcccaccc aataatcccc
     2761 caacctatct tggtaaattt gccgcacttg gtttgaatta accgagtcgg cgttgtaggg
     2821 atcgtgttcg taacggtcaa agcgatcagc actgcgaata taaacggcta cgccgccgtg
     2881 gtcgctggag atccataaat tctggtcttt atccagcata attttccaca aacggttatc
     2941 gcctaaactg tggggattat tatcttcgtg ctgataccac acggcggttt tacgggtttc
     3001 atcaattttg accaggccat tgtcggtggc catccataaa aaaccttctt tatcttgttt
     3061 tacatcgcgc accgtggcgg ttgctaaatc aaattgcaga gccacaggcg gcgtataaac
     3121 ggtaaattca ttttgatgtt cggcacgaat atacaacccc ccgccctgca cacccaccca
     3181 caggcgtttt ttattgtctt catacagcgc agtaataata cctttggtat tttccaaact
     3241 ttgcggcgta gg
//
LOCUS       DAOQGS010000472         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17257_length_3251_cov_4.075720, whole genome shotgun sequence.
ACCESSION   DAOQGS010000472 DAOQGS010000000
VERSION     DAOQGS010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17257_length_3251_cov_4.075720"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PKZ52_14875"
     CDS             complement(<1..812)
                     /locus_tag="PKZ52_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61104.1"
                     /translation="MTLHHANKPLQIANGFTATKTQAWIKTLEYGAIALALQCADWLL
                     ALLLEQTYVLAMGSLFIVYLIALRKISRLQLCLLITLQCTSAWLLDMDVRVLLGFSLA
                     QGVSLGIFWAFLHRYHLVLSDGVSATTPSEEYQKNTAPLERELLKNVTRMCSAWLIAS
                     TLAAGVFGVIVASGIKQFFIDMSAYWLANICTFVGLIMPIMTQLTQAAREDHQRKRVK
                     PYLTTLLTTCAFLIYSNVIINTTPYPFIYILLPMCLLAVRLPVTTIAMIIYGG"
     gene            complement(1257..2222)
                     /locus_tag="PKZ52_14880"
     CDS             complement(1257..2222)
                     /locus_tag="PKZ52_14880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG61105.1"
                     /translation="MNDNKQPLILIIEDNPSDLRVLGNLVNHLGEVIFATSGESGLIL
                     AQHRKPDLILLDVELPDINGFKICSELKKLQETEDSAVIFVTANNDEHSEVAALEAGA
                     VDYICKPYVAQIVRARVKTHLTLKKQASELQELANKDGLTGVFSRRYFDYQARVEINR
                     HARQCSPLSLVLIDVDHFKSYNDHYGHLKGDWCLKTIAQTLQSNSRRPGEFVARYGGE
                     EFVAILPNTNYREAEKYGRWICERVRSLALPHENSPTGRYISISAGLITITPDPTTLL
                     DTLITEADKALYQAKHNGRDQFIQGNISIASLAVNIFKNPQTSRI"
     gene            2679..3050
                     /locus_tag="PKZ52_14885"
     CDS             2679..3050
                     /locus_tag="PKZ52_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNG61106.1"
                     /translation="MNIAKMYKAMVRSVGAAGMTAALMLPVTAFAVEKPTVEPKTQEV
                     KSAEAKLKDGSAKEQAAVNINTADAATLAAGLNGIGIKKAEAIVAYREQNGKFTSADQ
                     LLEVKGIGKATLDKNRDKIKI"
BASE COUNT          850 a          679 c          849 g          873 t
ORIGIN      
        1 ccgccgtaaa taatcatggc gatggtagtg acgggcagcc gcaccgccag cagacacata
       61 ggcaataaaa tataaataaa tgggtagggc gtggtgttaa taatcacgtt gctgtaaatc
      121 agaaatgcac aggtggttag tagtgtggtt aggtagggtt taactctttt gcgctggtga
      181 tcctcgcggg ccgcctgggt tagctgggtc ataatgggca taatcaggcc cacaaaggta
      241 cagatattgg ctaaccaata ggcgctcatg tcgataaaaa attgtttaat accgcttgca
      301 acaatcaccc cgaatacacc ggcggcgagt gtgctggcaa ttagccaggc gctgcacatg
      361 cgggtgacat tttttaataa ttctcgctcc aagggagccg tgtttttttg atattcctcg
      421 ctcggtgttg tcgcactaac gccatcggat aacaccagat ggtatcgatg taaaaaagcc
      481 caaaaaatgc ccaggctaac cccttgcgcc aagctaaagc caagcagcac acgcacatcc
      541 atatcaagca gccaagctga tgtgcattgc agcgtaatca gcaggcataa ttgcaggcgg
      601 gaaattttgc gcagggcaat tagatagacg ataaaaaggc tgcccatcgc caatacatag
      661 gtttgctcta ataaaagtgc cagcagccag tcggcacatt gcagcgcgag tgcaatagcg
      721 ccatattcta gagttttaat ccaagcctga gtttttgtag cggtaaatcc atttgctatt
      781 tgtaagggct tattcgcgtg atgtaatgtc atggggcgta ggttagcgat tggcgagtgg
      841 gcaaacaggt gaatattttt tattgttata atttaaccgg ttgttaaggt gaattaattt
      901 gaggagtctc tgagcaatta ctgtttgcag gaacgcatta tttatagcag acaagcagga
      961 actgatggaa agttacctgt agctccgctg tgggcctcct gcccataggc tttaaaaatt
     1021 tcccgcctag cagtttgttg acactattaa cccggcggct aaatagatcg tcctgatcta
     1081 tttagccttc gctgccctaa aactggcgaa aatgcgcgga ttttcttgtt ttagcgcagc
     1141 tctaggcggt ttacacagcc ggggcggcat ccatgccgcc attattaacc cggcactaaa
     1201 tgccgacggg ttaataattg atgttctata gcgcgcatcg tccttctaag cgggctttaa
     1261 attcggctgg tttggggatt tttaaaaata ttcactgcaa ggcttgcgat gctgatattg
     1321 ccctgaataa attgatcgcg gccattgtgc ttggcttggt ataaggcttt atccgcttcg
     1381 gtgatcagtg tgtccagcag ggttgttggg tctggggtga tggtaattaa gcccgcgcta
     1441 atgctgatat aacgtcccgt gggggaattt tcatggggta gggctaggga tcgcacgcgc
     1501 tcgcaaatcc aacggccgta tttttcggct tcccgataat tagtattcgg cagaattgcc
     1561 acaaattcct cgccgccgta gcgggcgacg aattcaccgg ggcgccggga gttgctttgt
     1621 aatgtttggg cgatggtttt aaggcaccag tcgcctttta ggtggccata gtggtcgttg
     1681 taactcttaa aatgatccac atcaattaat accaggctga gcggggagca ttgacgggcg
     1741 tgccggttaa tctcaacccg tgcctggtaa tcgaaatagc ggcggctaaa tacaccggtc
     1801 aaaccatctt tattggccag ttcttgtaat tcgctggctt gttttttgag tgttaaatgg
     1861 gtttttaccc gcgcgcggac aatttgcgct acatagggtt tgcaaatgta atctacggcg
     1921 ccagcttcca aggcggctac ttcagaatgt tcatcattat tcgcggtgac aaaaataacg
     1981 gcggaatctt cagtttcctg cagtttttta agctcactgc atattttaaa accgttgatg
     2041 tcaggcagct caacgtccag taaaatcaaa tcaggttttc tgtgctgggc gagaataaga
     2101 ccggactcgc cggaggtggc aaaaataacc tcacccagat gattcaccaa attgccaagt
     2161 acacgcaggt cgctggggtt gtcttcgata attaaaatca agggctgttt attgtcgttc
     2221 acagttttaa tctcctaaag cagtgttcga ctggtgcggc acagtgttaa gtgcctgctt
     2281 gcgaaccggt agatagtgaa aaagtgacaa ttaaaattga gctgcgttta acttggggcg
     2341 ttgcttgttc gaatataatt tttagcttgg gaaatctaaa caagctctga tcaattagtt
     2401 tcctaatgat ctcgatagtt gtttgccagg ggttcaaccg aggtcttagc ttaagcaggc
     2461 tgcacagcgg ccatgtcttt tagtcaatgg gcctattagg ctgcaggggc ttgctcttca
     2521 agtttagcta acttggtctg attcgctagg cgggggtttg cgcgatattg cttacacaca
     2581 ttagcgttaa tatccgctag tgggcggcga ggctgatagt taggctagcc gcgtctgatt
     2641 gagatcagat aactcaatat tcttgtaagg agatcggtat gaatatagca aaaatgtata
     2701 aagcaatggt tcgttcagtg ggtgctgccg gtatgacagc ggctcttatg ttacctgtga
     2761 cggcttttgc ggttgaaaaa cccactgttg aaccgaagac ccaggaagtc aaaagcgccg
     2821 aggcaaagct taaagatggc agtgctaaag aacaggccgc cgtcaatatc aacacggcgg
     2881 atgcggcgac attggcggcg ggccttaacg gcataggcat aaaaaaggcg gaggcgattg
     2941 tggcctatcg tgaacaaaac ggtaaattta ccagtgcaga tcagctgctt gaagttaaag
     3001 gcataggtaa agcaacgctg gataaaaatc gcgataaaat aaaaatctaa cgccatcgaa
     3061 tagcaagcca gtattcaaac cggctgttaa tttttattta ggaagggtgg tgtaaggcag
     3121 ggacgctaaa gtgaaatgta catacaatgc ccgtgcgtat ttaaagtgcc aaaaccagag
     3181 tgttcgttct tggcggcagt attcaaaccg gctgttaatt tttatttagg aagggtggtg
     3241 taaggcaggg a
//
LOCUS       DAOQGS010000473         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17272_length_3249_cov_4.650595, whole genome shotgun sequence.
ACCESSION   DAOQGS010000473 DAOQGS010000000
VERSION     DAOQGS010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17272_length_3249_cov_4.650595"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2304
                     /locus_tag="PKZ52_14890"
     CDS             <1..2304
                     /locus_tag="PKZ52_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61107.1"
                     /translation="AAWSGPTPAILVNVRRQPGANVIAVTDQILALLPELQASLPAAV
                     TIEVLSDRTRSIRAALKDVKFELCLAVALVVMVIFLFLRSAAATLIPSLAVPLSLVGT
                     FGVMYLAGFSLNNLTLMALTIATGFVVDDAIVMIENIARHREMGKPARQAALDGAREI
                     GFTIISLTFSLLAVLIPLLFMGDVVGRLFREFAITLAVAILISAVVSLTLTPMLCARL
                     LGRPGGTAPAISHKATPASGWQAVLTARYSQALYWVVAHQRTMLALVVFTIGLTAFLA
                     LAIPKGFFPRQDTGLIQGISLGPANTGFAAMSARQEALVARLEADPAVASVASVVGVD
                     NSNTQLNQGRLLISLKPRAERSALSEVLERLQAGAEQAGMPLFLQAAQDLSLDDRASQ
                     SQYQLLLTSPDAELLANWLPQVVSTLNGLGALRDVASDQPPEVMGAYIAVDRDTASRL
                     GVSQAAVDEVLYNAFGQRLISTIFTQSKQYRVVLEVDSEDKGLARLEDLYVTGNGGQQ
                     IPLANLAHWSERPSSLLITRDKQFPAATISFNLAPGVSIGQAVNAIEDSLAQMDWPAG
                     VDKRFQGAARAFADALDNQLWLIAAALLSMYIVLGVLYESYLHPLTILSTLPSAGIGA
                     LAALWACGEDLGVIAIIGIVLLIGIVQKNAIMIIDFALDAQRNQGKSAAEAAVQACVL
                     RLRPIVMTSLAALLSAVPLVLGHGEGSELRRPLGISLIGGLLASQALTLFTTPVVYLA
                     FDRLRQWQLGPQKLNNRADLAPPGQVV"
     gene            2306..>3249
                     /locus_tag="PKZ52_14895"
     CDS             2306..>3249
                     /locus_tag="PKZ52_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61108.1"
                     /translation="MAREPMLKRAEPIWSVFVRRPVATSLLTLALALPGLVCFKLLPI
                     SALPMVDLPTIGVEASLPGAAPEIVASALATPLERALGRISGVTELTSTSAQGQTRIN
                     VQFDLDKDINSAAREVQAAINAAAADLPSGMLSKPSYRKSNASGAPILIIALTSTAIT
                     QERLYDLAFTHLGQAISQVSGVGDVAVNGSALRSIRVELDPNALTQRGISLEQVRTAI
                     ASANPNRPTGVIEGRQQRWQVAVNDQVRQAADLSELIIHQSSGQVVRLKDVARVEDSV
                     QERRNAGTSRGEPAVLLVIFGQPGANVGATAHSIKALLP"
BASE COUNT          613 a         1030 c          944 g          662 t
ORIGIN      
        1 gccgcctggt ccgggccaac cccggcgatc ctggtaaatg tgcgccgcca gccgggggcc
       61 aatgtgatag cagtgaccga ccagattctc gccctgctgc ccgagctaca ggctagcctc
      121 cccgctgcgg tgaccattga agtattaagt gatcgcaccc gcagcattcg cgctgccctt
      181 aaggatgtga aatttgagtt gtgcctggct gttgccctgg tggtgatggt tatttttctg
      241 tttctgcgca gcgctgcggc caccttgatt cctagccttg cggtgccgct gtcgctagtg
      301 ggcaccttcg gggtgatgta tctggcgggc tttagcctca acaatctcac gctgatggcc
      361 ctgaccatcg ccaccggttt tgtggtggac gatgccatag tgatgatcga aaacatcgcc
      421 cgccaccggg agatgggtaa gcccgcccgc caagcggctc tggacggcgc gcgggaaatt
      481 ggcttcacca ttatctccct gaccttttcg ctgttggcgg tattgatccc actgttattt
      541 atgggggacg tggtggggcg gctgttccgc gaatttgcca tcaccctggc cgtggcgatt
      601 ctaatttctg ccgttgtttc ccttacgctt acgcctatgc tgtgtgcccg tttgctcggc
      661 agaccggggg gcacggcccc tgccatttct cacaaggcaa cccccgcgag tggttggcaa
      721 gcggtgttaa ccgcccgcta tagccaagcg ctgtattggg tggtggccca ccagcgcact
      781 atgctcgcgc tggtagtgtt taccataggt ttaacggcct tcctcgcact ggctatccca
      841 aagggatttt tcccccgcca agacacaggt ttaatccaag gcattagcct ggggcctgcc
      901 aataccggct ttgccgccat gagtgcgcgc caggaagcgc tggtcgcccg cctagaggca
      961 gacccagccg tggcatcggt ggcctcggta gtgggcgtgg ataactccaa tacccagctt
     1021 aaccaagggc gcctgcttat aagcctcaaa ccccgcgctg agcgctcggc actgtcggaa
     1081 gtgctcgagc gcttacaggc tggcgctgag caggcgggga tgccgttatt tttacaagcc
     1141 gcccaggatt tgagcctgga tgaccgggcg agccaaagcc aatatcaatt gctgctgacc
     1201 agcccagatg ctgaactgct ggccaattgg ctgcctcagg tggtcagcac cttaaacggg
     1261 cttggggccc tgcgcgatgt cgccagcgac cagccccccg aggtgatggg cgcttatatt
     1321 gcggtagatc gtgatacggc ctcgcgtctg ggtgtaagcc aagcggcggt ggatgaagtg
     1381 ctctacaacg ccttcggcca gcggcttatc tccaccatct ttacccagtc caagcagtac
     1441 cgggtggtgt tggaggtgga tagcgaggat aagggtttgg cgaggctaga agacttatac
     1501 gtaaccggca atggcgggca acaaattccg ctcgccaacc tcgcccactg gagcgaaagg
     1561 cccagcagcc tgctgatcac tcgggataaa caatttcccg ccgccaccat cagttttaac
     1621 ctggcccctg gtgtttccat aggccaagcg gtgaacgcca tcgaggacag cctggcccaa
     1681 atggattggc ctgcgggggt agacaagcgc tttcaaggcg ccgcccgtgc cttcgccgat
     1741 gctctggata accaactctg gctgatcgcg gcggccctac taagtatgta tatagtcctg
     1801 ggcgtgctgt acgaaagtta cctgcaccct ttgaccattc tctccaccct gccctccgcc
     1861 ggcatcggcg ctttagccgc cctttgggcc tgtggtgaag acctcggcgt gatcgccatc
     1921 atcggcatag tgctgctcat cggcatagtg cagaaaaacg ccattatgat tatcgacttc
     1981 gccttggacg cccagcgcaa ccagggcaaa agtgctgcag aggccgcagt gcaggcctgc
     2041 gtgctgcgct tgcggcccat tgtgatgacc agccttgccg ccctgctcag cgcagtaccc
     2101 ttagtgttgg gccatggcga aggcagcgaa ctgcgccgcc ccctgggcat cagcttaatc
     2161 ggcggcctat tggccagcca ggccctcacc cttttcacca ctccggtggt gtatttggcc
     2221 tttgatcgcc tgcggcaatg gcaactgggc ccacaaaaac taaacaaccg tgccgatctg
     2281 gcaccgcctg gccaggtagt gtgatatggc ccgcgaaccc atgctcaaac gtgcagaacc
     2341 catttggtcg gtatttgtgc gccggccggt ggccaccagt ttattgaccc tggcactggc
     2401 cctgcccgga ctggtgtgtt ttaaattgtt gcccatcagc gccctgccga tggtggactt
     2461 acccaccatc ggggttgaag ccagcctgcc cggtgccgcg ccggaaattg tcgcctcggc
     2521 gctggccacg cccctagagc gcgccctcgg ccgcatcagt ggcgtcacag aactcacttc
     2581 caccagcgcc caagggcaaa cccgcattaa tgtgcaattc gacctggata aagacatcaa
     2641 cagcgccgcc cgggaagtgc aagcggccat aaacgccgcc gcggcagacc tgcccagcgg
     2701 catgctgagt aaacccagct accgcaagtc caacgcctcc ggggcgccca tcttaatcat
     2761 tgccctcacc tccaccgcca tcacccaaga gcgcctctac gacctcgcct ttacccatct
     2821 gggccaggcc atctcccagg tgagcggtgt gggcgatgtg gcggtaaacg gcagcgccct
     2881 gcgctcaata cgagtagagc tcgaccccaa tgcactcacc cagcggggca tcagccttga
     2941 gcaagtgcgt accgccattg ccagtgccaa ccccaatcgc cccaccggtg ttattgaagg
     3001 ccgccagcag cgctggcagg tggccgtcaa cgaccaagtg cgccaagccg ccgacctaag
     3061 cgagctgatc attcaccaga gctcaggcca agtggtgcgc cttaaggacg tagcgcgggt
     3121 ggaagattca gtacaggagc ggcgcaacgc cggcacctcg cggggggaac cggcggtatt
     3181 gctggtgatt tttggccagc ccggcgccaa cgtgggagct accgcccaca gcattaaggc
     3241 cctgctgcc
//
LOCUS       DAOQGS010000474         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17384_length_3234_cov_4.960365, whole genome shotgun sequence.
ACCESSION   DAOQGS010000474 DAOQGS010000000
VERSION     DAOQGS010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17384_length_3234_cov_4.960365"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="PKZ52_14900"
     CDS             <1..1771
                     /locus_tag="PKZ52_14900"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNG61109.1"
                     /translation="AVFARAQASYPGGEAPALGCLGDLLAVRMAWDWALLHWLQQHPR
                     LRSLASRFAQQFGQVAQWQASLNEQQRPLWVWQRALEISLQADLARKLCAPKPAAIAA
                     KALAVQAVFCIDVRSEPMRRALESVAPGIQTLGFAGFFGLPMEYQPYASDLRRPQLPA
                     LLTPALSARPLLADRQQRRLLAPLLRQHSQLHTGQQAAATFGWVEAKGVFEGLRLLAQ
                     SFGRGHLANPLNHFEGISGWQLWRDGAPLSLEATAQLLAGVLRHMGLTDGFAPRVLLV
                     AHASCTANNPLAASLDCGACGGQSGEVNVRVLAYLLNQPDVRAALTSTGITIPAQSRF
                     IPCLHNTTTDDLHWFGGDADVEPEAWHGWLKTAAERARALRAGSLDIKATSPERRAAA
                     FAARSRDWAELRPEWGLANNAAFIVAPRAATAHVDLQGRAFLHDYHWQRDSEFATLEL
                     IITAPMVVTQWINLQYYASTTDNLHYGSGNKLLHNVVGGNLGVFEGNGGDLRIGLPLQ
                     SLHDGEHWRHQPVRLSVYLAAPRPAIAAIIERHPNIADLINQDWLYLFQWDTEQLSIQ
                     RYYRGQWQPAPNTQQLKASPCTP"
     gene            1759..2679
                     /gene="cysD"
                     /locus_tag="PKZ52_14905"
     CDS             1759..2679
                     /gene="cysD"
                     /locus_tag="PKZ52_14905"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNG61110.1"
                     /translation="MHALSHLQALEAEAIGILREVAASFSKPCMFYSIGKDSTVMLHL
                     ARKAFWPGPVPFTLLHIDTTWEFAEMARFRDELVARWGLKLLVHVNREALAQGVHPIE
                     SGSVRYNDQMKTQALKQAMAQHRFDAALGGARRDEEKSRAKERVFSVRDAYQNWDPKN
                     QRPELWGIYNTQLNPGESLRVFPLSNWTELDIWLYILREGIDLVSLYFAQPRTTWRSD
                     ESGQVFILDDERMLPYLSPRERASIALRQVRCRTLGCYPQTGAVLSQAQDVAGIIQEL
                     LLSKTSEREGRMLDKDQVGSMEKKKREGYF"
     gene            complement(2737..>3234)
                     /locus_tag="PKZ52_14910"
     CDS             complement(2737..>3234)
                     /locus_tag="PKZ52_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61111.1"
                     /translation="FRDRATQCLDHTGLVLALVELLQQSVRPRLPFVVLELVASPQAG
                     LFTSQPLPHEVQVEFAERLKRYTKDDIGVVARWRGPCFIVLGTTTKEHLMVRLDLLRA
                     ALRINPLSYEDEPVYTDLIIASGVLNELEHAFDQLLNAWWRLHWRKRTGLVGVKPPKG
                     AQPGA"
BASE COUNT          591 a         1058 c         1003 g          582 t
ORIGIN      
        1 ggcggtattt gcccgcgccc aagccagtta ccccggtggc gaggcgcccg ctttggggtg
       61 cctgggggat ttattggcgg tgcgcatggc ctgggactgg gcgctattgc actggctgca
      121 gcagcatccc cgcctgcggt cactggccag ccgctttgcc cagcagtttg ggcaggtggc
      181 ccagtggcag gcgagcctaa acgagcagca gcggcccctc tgggtgtggc agcgcgccct
      241 ggagatcagc ctgcaggctg atcttgcccg caagctctgc gcccccaagc ctgcagccat
      301 tgccgctaag gccctcgcag tgcaggcggt attttgtatc gatgtccgct cagagcctat
      361 gcgaagagca ctggagtcgg tggcaccggg tattcaaacc ctggggtttg ccggtttttt
      421 tggcctgccc atggagtatc aaccctatgc cagcgacctt aggcgccccc agctgcccgc
      481 cctgctcaca cccgccttga gcgcgcgccc cctattggcc gatcgccagc agcgccgcct
      541 actggcgccc ctattgcgcc agcacagcca gctgcacacc ggccagcagg ccgccgccac
      601 ctttggttgg gtggaggcca agggcgtctt tgagggcttg cgcttattgg cccagagctt
      661 tgggcgcggc cacctggcca atcccttaaa ccactttgag ggcatcagcg gctggcaact
      721 ctggcgcgac ggcgcgcccc tcagtcttga ggccacggct caactgctgg ccggagtgct
      781 gcgccatatg ggtctaaccg acgggtttgc gccgcgggtg ctgcttgtgg cccacgccag
      841 ttgcaccgcc aacaaccccc tggcggcgag cctcgactgc ggcgcctgcg gcggccaatc
      901 cggcgaagtg aatgtgcggg tgctggccta tctgttaaat cagccggatg tgcgcgcagc
      961 ccttacatcc acagggatca ccatccccgc tcagagccgg tttatcccct gcctgcacaa
     1021 caccactact gatgacctgc actggttcgg cggcgatgcc gatgtggaac ctgaggcctg
     1081 gcacggctgg cttaaaaccg ccgccgagcg agcccgcgcc ctgcgggcgg gcagcctcga
     1141 cattaaggcc acaagcccag agcgccgcgc cgctgccttt gccgcccgca gccgggattg
     1201 ggccgagctg cggccggagt gggggctggc caataacgcg gcgtttatcg tcgcgccccg
     1261 cgccgccaca gcccatgtgg acttgcaggg gcgggcgttt ttgcacgact accactggca
     1321 gcgcgatagt gaatttgcca ccctggagct gatcatcacc gcccccatgg tggtcaccca
     1381 gtggatcaac ctgcagtact acgcttccac caccgataac ctgcactacg gcagcggcaa
     1441 taaactgctg cacaacgtgg tagggggcaa cctgggcgta tttgaaggca acggcggcga
     1501 cctgcgcatc ggcctacccc tgcaatccct ccacgatggc gagcactggc gccaccagcc
     1561 ggtgcgattg agcgtgtacc tcgccgcgcc gcgcccggcc atcgccgcca tcatcgagcg
     1621 ccatcccaac atcgccgacc tgatcaacca ggattggctc tatctgttcc aatgggacac
     1681 agagcaactg agcatccagc ggtactaccg gggtcaatgg caaccggcac ctaatactca
     1741 gcagctgaag gcctcgccgt gcacgcccta agccatttgc aggccctgga ggccgaggcc
     1801 atcggcatac tgcgggaagt ggcggcgagt tttagcaagc cctgtatgtt ctactccatc
     1861 ggcaaggact ccacggtgat gctgcacctg gcccgcaagg cgttttggcc cgggccggtg
     1921 ccgtttaccc tgctgcacat cgacaccacc tgggaatttg ccgagatggc gcgctttcgc
     1981 gatgagctgg tggcgcgctg ggggcttaag ctcttggtgc acgtcaaccg cgaggccctg
     2041 gcccaggggg tgcaccccat tgagtccggt tcggtgcgct acaacgatca gatgaaaacc
     2101 caagccctca aacaggccat ggcccagcat aggttcgatg cggccctcgg cggcgcccgc
     2161 cgggatgaag aaaaatcccg cgccaaggag cgggtgtttt ccgtgcggga tgcctatcaa
     2221 aactgggacc cgaaaaacca gcggccggag ctgtggggca tctacaacac ccagctgaat
     2281 ccgggtgaga gcctgcgggt gttccccctc tcgaactgga cggagctgga tatctggctg
     2341 tacatactgc gcgagggcat cgacctggtc tccctctact tcgcccagcc gcgcaccacc
     2401 tggcgcagcg atgagtccgg gcaggtattc attctcgatg acgagcgcat gttgccctac
     2461 ctaagccccc gcgagcgcgc ctccatcgcc ttgcgccagg tgcgctgccg caccctcggc
     2521 tgctacccgc aaaccggcgc ggtgctgagc caggcccagg atgtggcggg aattattcag
     2581 gaattactgc tgagtaaaac cagcgagcgg gagggccgta tgctggataa agaccaggta
     2641 ggctccatgg aaaagaaaaa acgcgagggt tatttttagc ctgtggagcc tgtcggggtg
     2701 gaatctaaac acggcaatcg ggtttaaccc cagaacttaa gcccccggct gggcgccctt
     2761 gggcggttta acccccacca aaccggttcg cttgcgccaa tgcaagcgcc accaggcatt
     2821 gaggagttga tcgaaggcgt gctccagttc gttgagtaca ccgcttgcga tgatgagatc
     2881 cgtgtacacg ggctcatctt catagcttaa ggggttgatg cgcagggcgg cccgcagcag
     2941 gtccagccgc accatcaggt gttctttggt ggtggtgcca agcacaataa aacaggggcc
     3001 gcgccagcgc gccaccacgc ctatatcgtc cttggtgtaa cgctttaagc gctcggcaaa
     3061 ctccacctgc acctcgtggg gcaggggttg gctggtgaat agacccgcct gggggctggc
     3121 caccagttcc aagaccacaa acggcaggcg cggccgcacg ctctgctgca gcagttccac
     3181 cagggccaat accaggcccg tgtggtccag gcattgggtg gcgcggtcgc gaaa
//
LOCUS       DAOQGS010000475         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17440_length_3228_cov_4.266625, whole genome shotgun sequence.
ACCESSION   DAOQGS010000475 DAOQGS010000000
VERSION     DAOQGS010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17440_length_3228_cov_4.266625"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKZ52_14915"
     CDS             complement(<1..544)
                     /locus_tag="PKZ52_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNG61112.1"
                     /translation="MTHSEKTKARNTHTPAFKKSLLAMCVVAAVQQNAVYAAEPTAET
                     EEIVVTGMRESLNSAQDIKKSADTFVDAISAKDIGALPDKSVTEALQRVPGVTVSKYA
                     APVDPDHFSAEGSGVVIRGLGQTRSEFNGRDTFTANSGSGLSFQDVSPELMGAVKVYK
                     NQTADMIEGGISGTVNLNTRK"
     gene            complement(668..1969)
                     /gene="tyrS"
                     /locus_tag="PKZ52_14920"
     CDS             complement(668..1969)
                     /gene="tyrS"
                     /locus_tag="PKZ52_14920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNG61113.1"
                     /translation="MSVDPTLLDDLQKRGLIAQMTGDQQLKEFLQSPRTLYAGFDPTA
                     DSLHIGSLVPLLALKRFQLAGHKPIALVGGATGLIGDPSFKAQERKLNTPDVVAQWVQ
                     RLKDQVSQFIDFNDSPSGAQVVNNLDWVGNLNVLDFLRDVGKHFSVNNMINKESVKQR
                     LDREGEGISFTEFTYMLLQSFDFAQLYKTFGCTLQIGGSDQWGNITGGVDLTRRLHGG
                     QVFGMTLPLVTKADGTKFGKTESGTVWLSAAKTSPYAFYQFWLATADADVYKFLKYFT
                     FLSVDEIDALEQRDRESGAKPQAQAVLAREVTQLVHGEKGLQAAMRITEALFCGELSA
                     LTAEDFTQLALDGLPASELKSEALAAQSLAQILTEAGAVQSGKQVKDALSSGSLLVNG
                     TALDAAALMDTSQVFSPDKSVHGKYFLVKIGKKKHHLFFIA"
     gene            2169..>3228
                     /locus_tag="PKZ52_14925"
     CDS             2169..>3228
                     /locus_tag="PKZ52_14925"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HNG61114.1"
                     /translation="MKIIKTIWLKLQNSDREAFPRWHLLIASGLVAGLVTLINAPAST
                     DTTAETQASSSEERPSVNTPLEGQMLSESDELLNPLATLIPKSNPLSFYEFDTTPQPS
                     EAAQDPLVKHEVVGKGDNLSSIFQRAGLDNKKLAEVLDSCKESKTLKQMHPGHRLTFT
                     VDPQGTLTRVDYQPNLLETRTFTRQGEKFVYSESKRKPDIQTKVASAVIQSSLYGAAK
                     TAQISDKLIYDMANVFAFDIDFALDLQPGDSFKILYEEAYLDGKKVGGEQLLAAEFIN
                     EGKVYKAVRYLDKDGQGQFYSDDGKALRKGFLRTPVEFARISSGFSLGRMHPILNKIR
                     AHKGVDYSAPTGTPIMAAG"
BASE COUNT          787 a          875 c          820 g          746 t
ORIGIN      
        1 gcttgcgagt attgaggttg accgtaccgg aaataccccc ctcgatcatg tccgctgttt
       61 ggttcttata aactttcaca gcccccatca attcagggga cacatcttga aagctcaaac
      121 ccgaacctga gttagcggta aaggtatccc ggccgttaaa ctctgatcgg gtttgcccca
      181 aaccgcggat caccacgcca ctaccttcag cagagaagtg gtcagggtca accggcgccg
      241 catattttga aacggttaca cctggtacgc gctgcagggc ctcggtgaca cttttatcag
      301 gtagggcgcc aatgtctttg gctgaaatgg cgtccacaaa tgtatcggcg gattttttaa
      361 tgtcctgggc gctgtttaag ctctcccgca tacccgttac aacaatttct tctgtctcgg
      421 cggtgggttc tgctgcataa acagcgttct gttgaacggc cgcaaccacg cacatagcca
      481 acagtgattt tttaaatgct ggtgtatgtg tgttgcgcgc cttggttttt tccgaatgag
      541 tcattattca gccccaatta ttattcatat aaatttcgtg ccagcacggg ggaagccccg
      601 tgaagactgg cgccaggatg gatagtagct agcgattccg gcggaagtca aactgacaat
      661 acgggtttta ggcaataaaa aagagatgat gctttttttt gcctatcttt accaaaaagt
      721 attttccatg caccgattta tcaggcgaga acacctggct ggtatccatg agcgcagccg
      781 catctagggc tgttccgttc accagcagcg agccgctgct aagtgcatct tttacttgtt
      841 tgccgctttg cacggccccg gcttcagtca aaatctgcgc gagggactga gcggccaggg
      901 cttcactttt caactcactg gcaggcaggc cgtccaaggc aagctgggta aaatcctcgg
      961 cggtcaatgc gctgagctca ccacaaaaca aggcttcagt gatccgcatg gccgcctgca
     1021 ggcccttttc gccgtgcaca agctgggtaa cttcacgggc gaggacagcc tgggcctgag
     1081 gcttggcacc gctttcgcga tcgcgctgct ctagggcatc aatttcatcc acacttaaaa
     1141 aagtaaaata cttcaggaac ttatagacat cagcgtccgc cgtggccaac caaaactggt
     1201 aaaaagcata gggggaggtt ttggcggcag agagccacac ggtgccactt tccgttttgc
     1261 caaatttggt gccgtcggcc ttggtgacca ggggtagggt catgccgaat acctggccgc
     1321 catgcaggcg gcgggtgaga tccaccccgc cggtaatatt gccccactga tcagagccgc
     1381 caatttgcag cgtgcagcca aaggttttgt acagctgcgc aaagtcgaag gactgcagca
     1441 gcatataggt gaattcggta aaggagatac cctccccctc ccgatccagc cgctgcttaa
     1501 ccgactcctt gttaatcatg ttattgaccg agaagtgttt gcccacgtcc cgcagaaaat
     1561 ccaatacatt gaggttgccc acccagtcta ggttgtttac cacctgagcg ccgctgggag
     1621 agtcgttaaa atcgataaac tggctgacct gatcttttag gcgctgcacc cactgggcaa
     1681 ccacatcagg ggtattgagt ttacgctcct gggctttaaa gctcgggtcg ccgatcaggc
     1741 cggtggcgcc ccccaccaaa gcgatgggct tgtggcctgc cagttgaaaa cgcttgagcg
     1801 caagcaaggg tacgagactg ccgatgtgca ggctgtctgc ggtggggtca aagcctgcgt
     1861 agagggtgcg aggggattgt aaaaattctt ttagttgttg gtcgcccgtc atctgggcaa
     1921 ttaggccgcg tttttgcagg tcatcaagca aagtcgggtc gacactcatg tttggtctcg
     1981 ctgaagtgaa cttttttaaa acgcgacacc atacagaaga tgctcagatg gctcaaaccc
     2041 tgaacggttg atccgcactt ttttgcctga gcttattcca ataaatttca tgcattttaa
     2101 gtctgaaggg tgcttcccta gaaattttct gctataaatc cacccgtgcc aacagctacg
     2161 tcctaacaat gaaaataatc aaaactatat ggctcaagtt acagaacagt gaccgcgagg
     2221 cctttcctcg ctggcatctg ttgatcgcca gcggcttagt ggcaggcctg gtcacattga
     2281 ttaatgcacc agcgtccacc gacactaccg ccgaaaccca agcttctagc agtgaagagc
     2341 gcccgagtgt gaatacaccc ctagagggcc agatgctgtc tgaatctgac gagctactca
     2401 acccacttgc aaccctgatc cccaagtcca accccctctc cttctacgaa ttcgatacta
     2461 ccccgcagcc gagcgaagca gctcaagacc ccttggtaaa acacgaagta gtgggcaagg
     2521 gcgacaacct ctctagtatt tttcagcgcg caggtctaga taacaaaaaa ctcgccgaag
     2581 tgctggacag ctgcaaagaa tccaaaaccc tcaagcaaat gcacccaggc cataggctga
     2641 cctttacggt tgacccgcaa ggtacactta cccgcgtgga ctaccagccc aatctgctgg
     2701 aaacccgcac attcacccgc caaggggaaa agtttgttta cagcgaaagc aagcgtaaac
     2761 cggatattca aaccaaggtc gccagcgctg ttatccagag ttctttatac ggcgccgcca
     2821 agactgcgca gatcagcgac aaactgattt acgacatggc caatgtgttc gccttcgata
     2881 tcgactttgc gctggacctg cagccgggcg atagcttcaa aatcctctac gaagaagcct
     2941 atttagacgg caaaaaggtt ggtggcgagc agttgctagc ggcggaattt atcaatgaag
     3001 gcaaggtata caaggcggtt cgctacctgg ataaagacgg tcagggccag ttttacagcg
     3061 atgatggcaa ggccttgcgg aaggggtttt tacgcacacc ggttgaattc gcgcgcatta
     3121 gctccggctt cagcttaggg cgcatgcacc ctattttgaa caaaattcgt gcacacaaag
     3181 gcgtggacta ttccgccccc accggcaccc ccatcatggc agcgggtg
//
LOCUS       DAOQGS010000476         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17565_length_3214_cov_4.373219, whole genome shotgun sequence.
ACCESSION   DAOQGS010000476 DAOQGS010000000
VERSION     DAOQGS010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17565_length_3214_cov_4.373219"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKZ52_14930"
     CDS             complement(<1..1115)
                     /locus_tag="PKZ52_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNG61115.1"
                     /translation="MSPNPSDPNASAHRQSLAALSLGALGVVYGDIGTSPLYTIKELF
                     APGHGVAVNAPDVIGAISAIVWLLIVVVSFKYALIVLRAGNQGEGGALALTALASNAT
                     AHKPRLKALILMLGLFGATLFYGDSLITPAISVLGAVEGLEIITPSLEPVVIPAALVI
                     LGGLFALQRQGTQVVGRLFGPVVLMWFLLLAGSGLWHILQYPDILQALNPLHAVQFVS
                     RQGAHLLVPLGAIVLAVTGAEALYADMGHFGPKPIRWAWTFVVLPALAINYMGQGALL
                     LQNPAAVNNPFYNLFSAQLLWPVLGLATLAAIIASQAVISGAFSMTRQAVQLGFLPRM
                     RCLHTSAAESGQIFMPAVNVMLLLGVVAAVLIFGSSSA"
     gene            complement(1244..1954)
                     /locus_tag="PKZ52_14935"
     CDS             complement(1244..1954)
                     /locus_tag="PKZ52_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG61116.1"
                     /translation="MSADILLIEDDDDLRHTLQSALVLEGYGVTSAVSLSEARALLKH
                     RPSHLPPWDVWLLDLNLPDGQGQELLALLPSSGSTACIVITAQSDEARVAQLLDAGAD
                     GYLVKPFGVLELFARIRLALRQRGNQNRAAPKRYKHQELEVDLLSHRVSLNGLPCHLT
                     PKEFALLSCLIARAGQLVSQRQLLAQVWGAEALEHSHYLRLYMGQLRQKLGDNALAPR
                     WIFTETGLGYRLAANELA"
     gene            complement(1962..>3214)
                     /locus_tag="PKZ52_14940"
     CDS             complement(1962..>3214)
                     /locus_tag="PKZ52_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNG61117.1"
                     /translation="RGSLVPHLQLHLWFWVSLTAVNGIVLALMAYLRQQLASARQLSA
                     QLTEVNQWHQVLLESHTVSSLGERFLTQLAASAEPLPGPLLLALKGLSPAAQGPYCLW
                     GPWQLEQLAGVEAAEAERRALGPGTGCYERWQDLVLPILGRKSAYGALVLPFAVPAAT
                     RRHWQLLCHQLGGFYDRRLARQKELLASAALEQERTSKAIIAAVSHDFRTPLATIIGA
                     SSSLLAQELKLTQDQQQAQVQRIYQQAQHLARVSQNLLQLARLGSDVAIARAWEDPAE
                     MLGSLLAQLPFRAQVQVQLPEAAPWVWCNSVLINQALANLLENAHQYGPKDAPIQLRG
                     RLQAGLWLWQVQDGGTGLSAEQAKARQRAFERGEEAAHPSGVGLGLALCEAIARAHQG
                     ALSFNPQPSSTWQLQIPQPPQPAA"
BASE COUNT          695 a         1019 c          977 g          523 t
ORIGIN      
        1 gcggaggagc tgccgaatat caacaccgcc gccaccacac cgagcagcaa cattacattc
       61 accgccggca taaaaatctg tccggattcc gccgccgagg tatgcaggca gcgcatacgc
      121 ggtaaaaagc ccagctgcac cgcttggcgg gtcatggaaa aggcgccgga gatcaccgct
      181 tgggaggcaa taatggccgc cagggtggca aggcccaaca cgggccagag caattgggcg
      241 ctaaacaggt tgtaaaacgg gttgttcacg gcggccgggt tctgcagcag cagggcgccc
      301 tgccccatat agttgatggc cagcgccggg agcaccacaa aggtccaggc ccagcggatg
      361 ggcttggggc cgaaatggcc catgtcggcg tagagggcct ccgcgccagt aacggcgagc
      421 acaatggccc ctaagggcac cagcaaatgg gcgccctggc ggcttacaaa ttgcaccgca
      481 tgcagggggt taagggcctg caaaatgtcc gggtactgca ggatgtgcca aaggccagag
      541 cctgccagga gtaaaaacca catcagcacc accggcccga ataggcgccc caccacctgg
      601 gtgccctggc gctgcagggc gaataaaccg cccaaaatca ccagggcggc gggaattacc
      661 accggctcta gggagggggt aataatctcc agcccctcca ccgcacccag cactgaaata
      721 gctggggtaa tcaggctatc gccataaaac agggtggcgc caaacagccc tagcatcaaa
      781 ataagggctt ttaaacgggg tttatgcgcc gtggcattgc tggccagagc agtcagcgcc
      841 agggcgcctc cctccccctg gttgcctgca cgcagcacga tcaaggcata tttaaaactc
      901 accaccacaa tcagcagcca gacgatggcg gagattgccc cgattacatc cggggcattt
      961 acggccaccc catggccggg ggcgaagagt tctttgatgg tatataaagg actggtgcct
     1021 atatcgccat acaccacgcc gagggcgccg aggctcaagg cggccaggct ctggcgatgg
     1081 gctgaagcat taggatcaga aggattcggg ctcacgatta aaactcccac agaaataaac
     1141 gatatgcctc gcaagcacgg gaggcattga gtgcaggatc agtatggcga gctgtgtgtt
     1201 aggaaagtgt atggattggc tctaacctaa gagccgaccg tgctcaggcc aactcattgg
     1261 ccgccagccg atagcccaac cctgtttcgg taaaaatcca gcgcggtgct agagcgttat
     1321 cgcccaactt ctgccgcagc tggcccatgt acaggcgcag gtaatggctg tgctccaggg
     1381 cttctgcccc ccacacctgg gcgagcaatt gacgctggga caccagctgg ccggcccggg
     1441 cgattaaaca actgagcagg gcaaactcct tgggggtcag gtggcagggc aagccgttga
     1501 ggctcacccg atgggagagc agatccacct ccagctcttg gtgtttatag cgcttaggcg
     1561 ccgcccgatt ctggttgccg cgctgacgca gggccaagcg aatccgcgcg aagagttcga
     1621 gcactccaaa gggcttcacc aaataaccat cggcccccgc atccaacagc tgcgccacac
     1681 gggcttcatc ggactgggcg gtaatcacaa tacaggcggt gctgccgctg cttggcaaca
     1741 gggccagcag ctcctggccc tggccgtcgg gcagatttaa atccagcagc cacacgtccc
     1801 agggcggcag gtggctgggc cggtgtttga gcagtgcccg cgcctcgctt agggatacgg
     1861 cgctggtcac cccataacct tctaagacca gggccgactg cagggtgtgg cgtaggtcgt
     1921 cgtcatcctc aatcaataaa atatcagcgc tcatcggagg cttacgccgc tggctgcgga
     1981 ggttggggaa tctgcagctg ccaggtgctc gaaggctgcg ggttgaagct gagcgccccc
     2041 tgatgggcgc gggctatggc ctcacacaga gccaacccca agcccacacc gctggggtgg
     2101 gccgcctcct caccgcgctc aaacgcgcgc tggcgcgcct tggcctgctc ggcactaagc
     2161 ccggtgccgc catcctgcac ctgccacagc cataaacctg cctgcaggcg cccccgcaac
     2221 tgaataggcg catccttggg cccgtactgg tgggcattct ccagtaggtt cgcgagggcc
     2281 tggttgatca acacactatt gcaccacacc cacggcgccg cctcgggcag ctgcacctgc
     2341 acctgggcgc gaaaaggcaa ttgggccagc aagctgccga gcatctcggc ggggtcttcc
     2401 caggcgcgcg caatagccac atccgacccc aggcgggcca actgcagcag gttctggctg
     2461 acccgcgcca agtgctgagc ctgctgataa atacgctgca cctgagcctg ctgctgatcc
     2521 tgagtcagct taagctcctg tgccaagagc gacgacgagg cgccgatgat ggtggccagg
     2581 ggcgtgcgaa agtcgtggga tacggcagcg ataatcgctt tactggtacg ctcctgttcc
     2641 aaagcggcac tggctagcag ctctttttga cgggccaggc ggcgatcata aaacccgccc
     2701 agctggtggc acagcagctg ccagtggcgg cgggtggcgg cgggcacggc aaacggcagc
     2761 accagcgccc cgtaagcgct tttgcgcccc agtataggca acaccagatc ctgccagcgc
     2821 tcgtaacagc cggtgcccgg cccgagggcg cggcgctccg cttcagccgc ctccaccccc
     2881 gccaactgct ccaactgcca aggcccccac aggcaatagg ggccctgcgc cgccggggat
     2941 aagcctttga gcgccagcag caaagggcct ggcaaaggct ctgcgctggc tgccaactga
     3001 gtgagaaaac gctcccccag gctgctgacc gtgtgggatt ccaacaatac ctggtgccat
     3061 tgattcacct cagttaactg ggcgcttagc tgccgcgcgc tcgccagctg ctggcgcaaa
     3121 taggccataa gcgccagcac tatgccatta acggctgtca agctgaccca aaaccacagg
     3181 tgcagctgca ggtgcggcac taagctgccg cgcg
//
LOCUS       DAOQGS010000477         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17665_length_3204_cov_3.087012, whole genome shotgun sequence.
ACCESSION   DAOQGS010000477 DAOQGS010000000
VERSION     DAOQGS010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17665_length_3204_cov_3.087012"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3204
                     /gene="dnaE"
                     /locus_tag="PKZ52_14945"
     CDS             <1..>3204
                     /gene="dnaE"
                     /locus_tag="PKZ52_14945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNG61118.1"
                     /translation="ITLIAMDSIGYKNIIEVISKGYLHGQIIGGTLIQMDWIREHSEG
                     VIALSGGRLGEVGQAILSGRLDLATEKIQTLKNIFPQRFYIEITRTQRPGEETYIHQV
                     VPLADQFEIPLVATNDVRFLAQSQFEIHEARVCITEGRTLTDPRRERIFTDAQYLKSP
                     AEMVELFSDLPDAVSNSVEIAKRCSLNIQLGKYFLPEYPIPEGMTENEFFADITRKGL
                     DKRLAKLFDVSAVDFAQRRKPYDERINFELDIITQMGFAGYFLIVMDFIGWAKRHDIP
                     VGPGRGSGAGSLVAYALDITDLDPLQYDLLFERFLNPERVSMPDFDIDFCMDNRDAVI
                     GYVADNYGRDAVSQIITFGTMAAKAVVRDVARVQGKSYGLADKLSKMIPADPGMTLKA
                     AYEQEEILRDFLAGDSDAQEIWDMALQLEGLTRNVGKHAGGVVIAPTKLTDFAPLYCD
                     ETGSGLVTQYDKGDVEEAGLVKFDFLGLRTLTIIDWAKKMIDKRRLQQGLEPLDISLL
                     PLDDVATYNLLKKAETTAVFQLESRGMKDLIKRLQPDNIEDLIALVALFRPGPLQSGM
                     VDDFINRKHGRAKVAYPDAKYQHESLKPILEPTYGVIVYQEQVMQIAQVLAGYTLGGA
                     DLLRRAMGKKKPEEMAKQREVFQDGAKNKGIDPELAIRIFDLVEKFAGYGFNKSHSAA
                     YALVSYQTAWLKAHYPSQFMAATMSSDMDKTDKVVTFIEEVRAMGIALLPPDVNRGEF
                     HFTVDERDNIIYGLGAIKGLGEGPVDNIIAARKDGPFLNLFDFCARVDVRKVNKRALE
                     ALVRSGALDTIGPQVDVDYDRAVLWAALSEAVKTAEQHHANKNAGMMDLFGQVETVSP
                     GNVDVYADYAQQKRWSIKERLNGERDTLGLYLTGHPIDEYEQELTHLISSRLSHLKPE
                     KAKQTVAGLVVAFRVMKTKKGDNMGFVTLDDRTGRLEVAVFADIYSAHRDKLVKDALL
                     VIEGQVSHDDFSGGLKMRADAVFPLAEARETKVSALRLRLRPSTVASLQGDELKQCLQ
                     RHTPGACKVVVDYQLPRVQGDVSLGDAWRVRPSDELLA"
BASE COUNT          808 a          652 c          890 g          854 t
ORIGIN      
        1 cattacactt attgcgatgg attcgattgg atataaaaat atcattgagg ttatttcaaa
       61 aggttatctg cacgggcaga taataggcgg gacattgatc caaatggatt ggattagaga
      121 gcatagcgag ggggtaatag ccctttctgg tggacgtttg ggggaggtgg ggcaggcaat
      181 tttatccggg cgattggatt tggctactga aaaaatacag acgctgaaaa atatctttcc
      241 gcagcggttt tatattgaaa ttacccgtac ccaacgccct ggagaagaaa cctatatcca
      301 tcaagttgtg cccttggctg atcaatttga aataccgctt gtggcaacca acgacgtacg
      361 ttttctggct caatcgcaat ttgaaattca tgaagcccgt gtgtgtatta ccgaagggcg
      421 tacgctcacc gatccgcgca gagagcgtat ttttaccgat gcgcagtatt taaaatcacc
      481 agctgaaatg gtggagcttt ttagcgattt gccggatgct gtgagtaata gtgtcgaaat
      541 cgccaaacgt tgtagtttaa atatccagtt gggtaagtat tttttgcctg agtacccgat
      601 accggaggga atgacggaga acgaattttt tgctgatatt acgcgcaagg gactcgataa
      661 gcgcttagca aagttgtttg atgtgagtgc tgttgatttt gcacagcgaa gaaaacccta
      721 tgatgagcgt attaatttcg agctcgatat tattacgcag atgggatttg ccggttactt
      781 tttgattgta atggatttta tcggctgggc taaacgccac gatattcctg tcggccctgg
      841 ccgtggctcg ggggctggtt ccttagtagc ctatgcgctg gatattactg acctcgatcc
      901 attgcagtac gatttgctgt tcgagcgctt tttaaatcct gagcgcgtat ccatgccgga
      961 ttttgatatc gatttctgta tggataatcg cgatgccgtt attggttatg tggctgataa
     1021 ctatgggcgc gatgccgtct cacaaattat tacctttgga accatggcag ctaaagcggt
     1081 ggtgcgcgat gtggcgcggg tgcaggggaa atcatatggc ttggcggata agttatccaa
     1141 aatgatcccc gctgaccctg gcatgacttt aaaagcggcc tatgagcagg aagaaattct
     1201 gcgggatttt ttagccgggg attcggatgc ccaagaaata tgggatatgg ctctgcagct
     1261 tgaaggcttg acgcgcaacg tcggcaaaca cgccggggga gttgtaattg caccgactaa
     1321 attaaccgat tttgcaccat tgtattgtga cgaaaccggt tctggcttgg tgactcaata
     1381 tgacaaaggt gatgttgaag aggcgggcct agttaaattc gactttctgg gtttgcgcac
     1441 gcttacaatt atcgattggg caaaaaagat gatcgataaa aggcgtttgc agcaagggct
     1501 tgaacctttg gatatcagcc tattaccgct cgatgatgtt gccacctata atttattaaa
     1561 aaaagctgaa acaactgcgg tattccagtt ggaatcccgc ggcatgaaag atttaattaa
     1621 gcgtttacag ccggataaca tcgaagacct aatcgcattg gtggcacttt ttcgcccagg
     1681 ccctttgcag tcgggcatgg tggatgactt tattaaccgc aaacacggtc gcgctaaggt
     1741 tgcctaccca gatgctaaat atcaacacga gtcgttaaaa cctattcttg aacccactta
     1801 tggcgtaatt gtgtaccaag aacaggtcat gcagattgcc caggtattgg ctggctacac
     1861 gcttggcggc gcggatttat tgcgccgagc catgggtaag aaaaagcctg aagaaatggc
     1921 gaagcagcgg gaagtgtttc aggacggcgc gaaaaataaa ggtattgatc ctgaattggc
     1981 catccgtatt tttgacttgg tggagaaatt tgcagggtac ggatttaata aatcccactc
     2041 ggcagcctac gcgctggtgt cttatcaaac ggcttggtta aaggcccatt atccatcgca
     2101 gtttatggcc gctaccatgt catcggatat ggataaaacc gataaggtgg tgacttttat
     2161 tgaagaagtg cgcgcaatgg gaattgccct gttgccaccg gatgttaatc gcggtgagtt
     2221 tcattttacg gtggatgagc gcgacaacat tatttatggt ttgggtgcca ttaagggttt
     2281 gggtgaaggc ccggtggata atattattgc ggcacgaaag gatggcccat ttttaaattt
     2341 atttgatttt tgcgcgcggg tggatgtgcg taaagtcaat aagcgtgcgt tggaggcttt
     2401 ggtgcgcagc ggtgcgctgg ataccattgg gccacaggtg gatgtggatt atgaccgcgc
     2461 agttttgtgg gcggccttat cagaagcggt gaaaacggct gaacaacacc atgctaataa
     2521 aaatgccggc atgatggatt tattcggcca agtggaaacc gtctcgccgg gcaatgtgga
     2581 tgtgtacgct gattatgcgc agcaaaagcg ctggagtatt aaagagcgtt taaatggcga
     2641 gcgcgatacc ttggggcttt atttaaccgg gcaccccatt gatgaatacg agcaggaatt
     2701 aacgcatttg atatccagcc gcctatccca tttaaaacct gaaaaagcca aacaaacggt
     2761 agcaggttta gtggtggcgt ttcgcgtgat gaaaaccaaa aaaggcgaca acatgggttt
     2821 tgtgaccctg gatgatcgca ccgggcgttt ggaggtggcc gtgttcgccg atatttattc
     2881 ggcccatcgc gataaattag taaaagatgc cctgctcgta atcgaggggc aggtgagcca
     2941 cgatgatttt agtggcggct taaaaatgcg cgcagatgcg gtttttccgc tcgctgaggc
     3001 ccgtgaaacc aaggtgagtg cattgcgctt gcggctgcgc cccagcactg tagcctctct
     3061 gcagggggat gagcttaaac agtgtttaca gcggcataca cctggagcct gtaaggtagt
     3121 ggtcgattat caactgccgc gggtgcaggg cgatgtgagc ttgggcgatg cctggcgtgt
     3181 gcgtcccagc gatgaactgc tggc
//
LOCUS       DAOQGS010000478         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17672_length_3203_cov_4.249047, whole genome shotgun sequence.
ACCESSION   DAOQGS010000478 DAOQGS010000000
VERSION     DAOQGS010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17672_length_3203_cov_4.249047"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKZ52_14950"
     CDS             complement(<1..744)
                     /locus_tag="PKZ52_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNG61119.1"
                     /translation="MSDLMQLHGAAYWLPIIFSGLMVLAFFIYGILDGYDLGVGILLP
                     ADNEGERDIMIASIGPFWDANETWLVLGVGIMLIAFPAAHSVVLFHLYLPVTIMLAGL
                     ILRGVAFDFRAKAPEDHKTLWDRAFKLGSILATLAQGYMLGIYVMGFEQSILAYLFAC
                     LSAICVTAGYSFIGGAWLIMKTEGALQQRAIRWTRGCGYLTALGIVLVSIVNPLISPT
                     IFAKWFSGSAWFLLLLIPALCAGLFLALMR"
     gene            complement(741..2135)
                     /locus_tag="PKZ52_14955"
     CDS             complement(741..2135)
                     /locus_tag="PKZ52_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNG61120.1"
                     /translation="MLLDTLLLDTLVLSRMQFAANISFHILFPSITIALGWFLFYFKW
                     QYGRSQDEVWMRIYRFWVKIFALTFALGVVSGITMSFQFGTNWPGFMQKVGNIAGPLL
                     GYEVLTAFFLEATFLGIMLFGIGRVSNRLHTLATFLVAFGTSLSAFWIIALNSWMQTP
                     AGFEMRDGVAHPVDWWAIIFNPSMPYRLAHMLTASGLTAGFLIAGVCAYRILKGDPKP
                     APRKALKIAVFANSVLIILQIFLGDLHGLNTLQHQPAKIAAMEGIWQTERGAPLLLFA
                     IPDAASQSNLFAVEIPKAASLILTHDRNGELKGLNEFTGHTPPVKPLFFGFRIMVGVG
                     VLMLAVAWVSSFYLWRRCELPVWLLKVSVTMTFSGWIATLAGWYVTEIGRQPYLVTGV
                     LKTADAVTQVASDNVALSLAIYLSLYGALLFAYVRTIFVMARKAVLVDAPQQLTTLQE
                     QLLANPKLKLEQSL"
     gene            complement(2277..2828)
                     /locus_tag="PKZ52_14960"
     CDS             complement(2277..2828)
                     /locus_tag="PKZ52_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HNG61121.1"
                     /translation="MVTGLTPMIQSCVLHFGEMGSRWGINRTVGQMYALFVLTQQPLC
                     ADQIADSLGFSRSNVSMGIKELLSWELIKLQHLPGERREFYSAPGDVWDIAKTLIEQR
                     RKREIDPTLSTLRNLLLEAPANETEAYAQQRMRDMHELIEMLTIWTTEIQRMDAASLN
                     KLSKLGSNIGKVLDMKDKIFGRS"
     gene            2999..3190
                     /locus_tag="PKZ52_14965"
     CDS             2999..3190
                     /locus_tag="PKZ52_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61122.1"
                     /translation="MYALIRDLLFRLDAEVSHELTLDWLGAAQRLKLLPLFASPMPQA
                     PVEVFGLKNSFKIAKFQPK"
BASE COUNT          888 a          814 c          766 g          735 t
ORIGIN      
        1 cctcatgaga gctaaaaata aaccggcgca cagagcaggg attaacaata ataaaaacca
       61 ggccgagccg ctaaaccact ttgcaaagat tgtggggctt atcagcgggt tgacgatact
      121 cactaataca atgcccagcg ccgttaaata accgcacccg cgcgtccagc ggatggcgcg
      181 ctgttgcagg gcgccttccg ttttcataat taaccaagcg ccaccgataa aactataacc
      241 ggcagtgaca caaatagcgc ttaaacaggc aaataaatag gccaatatgc tctgctcaaa
      301 ccccattaca taaataccca acatatatcc ttgggcaagc gtggctaaaa tcgagccgag
      361 tttaaaggcg cgatcccaca gggttttatg atcctccggt gctttggcgc gaaagtcaaa
      421 ggccacgccg cgcaggatta accccgccaa cataatggtt accggcagat acaaatgaaa
      481 taacaccaca ctgtgggctg cgggaaaggc gattaacata atgcctacgc ccaataccag
      541 ccaggtttca ttggcatccc aaaatggccc aatcgaggca atcataatat cccgctcgcc
      601 ttcattatcc gccggcaata aaataccaac tcctaaatca tagccatcca aaatcccata
      661 aataaaaaac gcgagtacca tcaggccgct aaaaataatg ggaagccaat aggcggcgcc
      721 gtgaagttgc attagatcgc tcataggctt tgctccaatt ttaatttggg gttagcaagc
      781 aattgctcct gcagcgttgt gagttgttgt ggcgcatcca ccaacaccgc tttgcgggcc
      841 ataacaaaaa tagtacgcac ataggcaaat aaaagcgcgc cgtagaggct gagataaatc
      901 gccaaagaga gcgctacatt gtcggaggct acctgagtta cggcatctgc agtttttaat
      961 accccggtga ctaaataagg ttggcggcct atttcggtta cataccagcc tgccaaggta
     1021 gcgatccagc cagagaaggt catggtaaca ctcactttta acagccaaac gggtaattca
     1081 cagcggcgcc agaggtaaaa actactgacc caagccacag ccaacattaa taccccaacc
     1141 cccaccatga tgcgaaagcc aaaaaataac ggttttaccg gcggcgtatg gccggtaaat
     1201 tcatttaagc cctttaattc gccattgcgg tcgtgggtta aaatgaggct cgcggcttta
     1261 ggtatttcca ctgcaaataa attactttgg ctggctgcat ctggaatggc aaataacaac
     1321 aagggcgcgc ctcgttctgt ctgccatatc ccctccatag cggcaatttt ggcgggttga
     1381 tgctgcaggg tatttaggcc gtgtaaatcc cccaaaaata tttgcagaat aatcagtaca
     1441 ctattggcaa atacggcaat ttttagtgct ttgcgggggg caggtttagg gtcgcctttt
     1501 aaaatgcggt aggcgcacac cccggcaatt aaaaaaccgg cagtcaggcc cgaagctgtg
     1561 agcatatggg ctaatcgata gggcatggag gggttaaaaa taatcgccca ccaatccacc
     1621 gggtgggcaa cgccgtcacg catctcgaac cctgcgggcg tttgcatcca tgaattcagc
     1681 gcaataatcc aaaaggcgga taggcttgtg ccaaaggcca ctaaaaatgt cgccagggta
     1741 tgtaagcggt tggacactcg gccaatgcca aacaacataa tgcctaaaaa tgtggcttct
     1801 aaaaaaaacg ccgtgagcac ttcataaccg agtagggggc ctgcaatatt gccgaccttt
     1861 tgcataaacc ccggccagtt ggtgccaaat tgaaaactca tggtgatgcc actcaccacg
     1921 ccgagcgcaa acgttaaggc aaatattttt acccaaaagc ggtaaatgcg catccagact
     1981 tcatcctggc tgcggccgta ttgccactta aaataaaaca gaaaccagcc gagcgcgatg
     2041 gtgatgctgg ggaataaaat atgaaaggaa atattagcgg caaactgcat ccgcgataat
     2101 acgagggtat ccaataaaag ggtatctaat aacatggcag tctcctcaag tacaacaaag
     2161 gggttacagc actatttatt acgcgcttga gccgttgtgc ccacagcaaa tgtatttatt
     2221 ttctggggtt taaacggcga cgagccaaac gctcaattgc ctatcggccg gtttcgtcac
     2281 gagcgtccaa atattttgtc cttcatatct aataccttgc ctatgttgct gcctaattta
     2341 gacagcttat ttaggctggc cgcgtccatg cgttgaatct ccgtggtcca gatggtgagc
     2401 atttcaatta attcatgcat atcccgcatg cgctgttggg cgtaggcctc agtttcgttg
     2461 gcgggcgctt ccagcaataa attgcgcagg gtagaaaggg ttgggtcgat ttcccgtttg
     2521 cgccgttgct caatgagggt tttagctata tcccacacat cccccggtgc gctgtaaaac
     2581 tctcggcgct cgccgggcaa gtgctgcaat ttaatgagtt cccaggacaa taattcctta
     2641 atgcccatgc tgacgttgga gcgggaaaag cccaggctat cggcaatttg gtcggcacag
     2701 aggggctgct gggtaagcac aaatagggcg tacatctgtc ccacggtgcg gttgatgccc
     2761 cagcggctgc ccatctcacc aaagtgcagc acacaggact gaatcatagg ggtgaggcca
     2821 gtgaccattt cttaattcct gtaatttcag tatttgctga aattacagga aggtttgatc
     2881 tgcgtcaagt taaaaccttg tattagttcc aagcgagcgg tgcgcttggg gcgggactta
     2941 agtggctgcg ccggtcgctt taggctaata tcgccgcctt ttgcccagga gcctcgccat
     3001 gtatgccttg attcgcgacc tgttatttcg cctcgacgcg gaagtctccc atgagttaac
     3061 cctggactgg ctgggggccg cccagcgctt aaagctcctt cctttatttg ccagccccat
     3121 gccccaagca ccggtggaag tatttggcct taaaaatagc ttcaagatcg ccaaatttca
     3181 accaaaatag cttcaaagca aaa
//
LOCUS       DAOQGS010000479         3199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17706_length_3199_cov_4.614822, whole genome shotgun sequence.
ACCESSION   DAOQGS010000479 DAOQGS010000000
VERSION     DAOQGS010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17706_length_3199_cov_4.614822"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKZ52_14970"
     CDS             complement(<1..289)
                     /locus_tag="PKZ52_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61123.1"
                     /translation="MKKLIFWVSFFMTFLTSLMAGAKEPVMFERVIHLPDTEIRFSMT
                     EDFSKDMPAAPFVEKVTAHMLGELKMPGDRFVIGRRWWDLKPQGFFKKNWGS"
     gene            complement(295..1470)
                     /locus_tag="PKZ52_14975"
     CDS             complement(295..1470)
                     /locus_tag="PKZ52_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61124.1"
                     /translation="MGNDFSKLTMGVAGVNLQCHPQELMRLSMAPLVEEPLDSVSAYG
                     YQLWLTGYSDMVVRKERSSAFGMGRQLTQDLRSLKANPRMLSNHKLKDSVDTWSSSGM
                     FYRMDYKVCNGQIFIFNIQPLDHIQKSRDLLEKPSLYRVAKNSEGGWQIKGKVDVAEA
                     GYGAVNGQSNNLAKATWLMGAHLEFEFGDQVKEFTLFHNPSVGGPGDTWESFRDKLGI
                     TTPVTKVFADKLLAAQQANHTIKWVAHSQGGVIFAEAVRTILKKNKKQTGILGKQSIA
                     LHGNANNNFRSSFLFDKAGIEVIASRANTYDLVYVFAGFNTLNPWRVIGAAVYWRHVF
                     SGSVNQSTHTMQHKDYDAWNREMTFGAGRGRSPIQKGFHAVDQLGRKAIKAIPNYLK"
     gene            complement(1509..>3199)
                     /locus_tag="PKZ52_14980"
     CDS             complement(1509..>3199)
                     /locus_tag="PKZ52_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61125.1"
                     /translation="SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGFVFSGSFTPLTNQ
                     GPEARVTSETITITGVNALTSLRFTGDANAEFSLNGGPFSKESDVTLKPGSHTLTARL
                     VTSGSGSTPVSASFTIGSQTLAFTATTRADVIAPTGTISFPPKISLTEGATVIMRGTV
                     KDEANGSGVKSVKINNQTATFDADKGTWELKAATLTPGSQVLTLNVEDGAGNSSRAAS
                     VNIMQGDTNITFPERSGVDFVGPQTVAWDNLDGRNRALVMDVDAKALIAVDLNTGKRE
                     VLSDNTTQPEIPFIYEGKGYNAGSIVLDRKAGKVYAGLSIDVDNLESTLDHSIIGVDV
                     KTGGRSFVSSNVSYETAEIVLENTSAEYRIFSGDSKQDYINVWDLNRNEASNYSSSSL
                     GIPNSINPFNNVGSIAIDSPRKRLLVTSLSGDQFVFSVDIASPVSASAETKGARSIFS
                     DASVPNNENQFTQNDNYVLTSIRVDADRSRALMVDRLKPAIFELALSDDKTKDGARSV
                     FSDNATANKLTNPYGLHIESGMPYALLVDKGTKALMAVDLLSGERVFISKTSVN"
BASE COUNT          875 a          802 c          628 g          894 t
ORIGIN      
        1 tacttcccca atttttttta aaaaaaccct gaggtttcag atcccaccag cgacggccaa
       61 ttacaaaacg atccccaggc atctttagct cacccaacat atgtgccgtt actttttcaa
      121 caaaaggtgc cgcaggcata tctttagaaa aatcttcagt catactaaaa cgtatttcag
      181 tgtccggcaa atgaattact cgctcgaaca ttacaggctc tttcgcgcca gccattagcg
      241 atgttagaaa tgtcataaaa aaagagaccc aaaaaataag ttttttcata gtgtttattt
      301 caagtagtta ggtatcgcct taatcgcttt acgccccaac tgatcgactg catgaaagcc
      361 tttctgaata gggctacggc ctcgcccagc cccaaaagtc atttccctgt tccatgcatc
      421 atagtcttta tgctgcattg tatgagtgct ttgattaact gagccagaaa atacatgccg
      481 ccagtaaact gcggcaccta tcactctcca cggattaagc gtgttaaacc ctgcaaacac
      541 atacaccaaa tcataggtgt ttgctcgact ggcgatcact tcaataccgg ctttatcaaa
      601 taagaaactt gagcggaaat tattattagc attaccgtgc aaagctatcg actgcttacc
      661 caaaatacct gtttgttttt tattcttttt aagaatagtg cgcacggcct ccgcaaaaat
      721 tacccctccc tggctatgcg caacccactt aatggtatga ttagcctgct gagcagccag
      781 caatttatct gcaaaaactt tagttactgg tgtggtgata cctaatttat cgcgaaaaga
      841 ttcccaagta tcgcccggcc caccgacact tggattgtga aacaaggtga actctttcac
      901 ctgatcccca aattcaaatt ctaaatgtgc ccccatcagc catgtcgcct tggctaaatt
      961 atttgactgt ccatttaccg caccgtaacc cgcctcagcc acatccactt tgcctttaat
     1021 ttgccaacca ccttcactat ttttagccac cctgtaaaga ctaggttttt ctaatagatc
     1081 tcttgatttt tggatatgat ctaagggttg aatattaaaa ataaaaatct ggccgttaca
     1141 gactttgtag tccatgcgat aaaacatgcc actgctactc caagtgtcta cactgtcctt
     1201 tagtttatgg tttgatagca ttcgaggatt ggcctttaaa gatcgtaaat cttgcgttaa
     1261 ctgccttccc ataccgaatg cagaactccg ctcctttctc actaccatgt cgctataacc
     1321 cgtcaaccac aactgataac cgtaagcgct taccgagtcc agcggctctt ctaccaaagg
     1381 agccatactc aatcgcatca gttcttgtgg gtggcactgc aaattcacac ccgcaacacc
     1441 cattgttaat ttagaaaaat cattacccat aaccacctcc taatataatc ctgataactg
     1501 cccacaaatt aattcacaga ggttttggaa ataaatacac gctcaccgct taacaaatcc
     1561 acagccatta acgcttttgt acccttatct actaaaagcg catacggcat acccgattca
     1621 atatgtaagc catacgggtt ggtgagttta ttggctgtag cattatcgga aaaaacactt
     1681 cgcgcaccgt ctttggtctt atcgtcgctt aacgctaatt caaaaatagc aggctttaaa
     1741 cgatccacca ttaatgcacg acttctatct gcatcaactc taatggaagt taaaacataa
     1801 ttatcgtttt gggtgaattg attttcgtta tttgggacag atgcatcgga aaagatagac
     1861 ctagctccct tggtctcagc cgacgcactc actggcgagg caatatctac agagaaaaca
     1921 aattgatcgc cacttaaact cgtaaccagg aggcgtttgc gaggagaatc tattgcaata
     1981 cttccgacgt tgttaaatgg attaatgctg ttaggaatac ctaaagaaga ggagctgtag
     2041 tttgacgcct catttctatt caaatcccag acattaatat aatcttgctt agagtcgccc
     2101 gaaaatattc tatattccgc actcgtattc tctagaacta tctctgcagt ctcgtatgaa
     2161 acattactag aaacaaaaga ccgccctccc gtcttcacat cgactccaat tattgagtga
     2221 tccaacgtgc tttctaaatt atcaacatct atcgacaatc ccgcataaac cttcccagct
     2281 ttccgatcta aaacaatcga acctgcgtta tagcctttac cttcataaat aaagggaatt
     2341 tctggttggg tggtgttatc acttaacact tctcgtttgc cagtattcaa atccaccgca
     2401 attaatgctt ttgcatccac atccattacc agtgcgcgat tgcgtccatc taaattatcc
     2461 caagccacgg tttgcggccc cacaaaatcc acaccgctgc gctcggggaa cgtaatattg
     2521 gtatcacctt gcataatatt cacgcttgcg gcacggctac tattgccagc gccatcttct
     2581 acattcagcg ttaaaacttg tgaaccgggg gttagtgtgg cggcttttaa ttcccaggtg
     2641 cctttgtcgg cgtcgaaggt tgcggtttga ttatttattt ttacgctttt tacgccactg
     2701 ccgttggctt cgtctttaac cgtgccacgc atgatcacgg tggcaccttc ggttaggctg
     2761 atcttgggcg gaaagctaat ggtgcctgtg ggcgcaatga catccgcgcg ggtggtggcc
     2821 gtaaaggcta gggtttggct gccaatagtg aaactggcgg ataccggggt gctaccgcta
     2881 ccactggtga ctagccgtgc ggtaagcgtg tggctgccag gcttgagggt tacatcggac
     2941 tccttagaga agggtccgcc attcaatgaa aattcagcat tggcatcccc cgtgaatctc
     3001 aggctagtca acgcattcac gccagtaatg gtgatggtct ccgaggtaac acgcgcctct
     3061 ggcccctgat tggttaaggg tgtgaagctg ccactaaaca caaagccgct ggaactggag
     3121 gatgagctag aacttgagct ggaagagctg gagctagaac ttgacgagct agagcttgaa
     3181 ctggatgaac tggagcttg
//
LOCUS       DAOQGS010000480         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17718_length_3198_cov_4.285078, whole genome shotgun sequence.
ACCESSION   DAOQGS010000480 DAOQGS010000000
VERSION     DAOQGS010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17718_length_3198_cov_4.285078"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..2369
                     /gene="fliD"
                     /locus_tag="PKZ52_14985"
     CDS             336..2369
                     /gene="fliD"
                     /locus_tag="PKZ52_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNG61126.1"
                     /translation="MIDNNIVKSLGAGSGVDSANIIKQLKEIEKAGPQARIDAKKLKA
                     ETQISDFGLISSSLTALKTAATALTDKEGFFSKSASYTPSEAFTPVKLDTKVASGTYA
                     FDVTQIAKSQSLSSSKFSSLSDAVGTGTLTFELGSWDSSSTNFTLDGTKTGTSVTIDS
                     SNNTLQGLRDAINKAAFGVQASVVKDGTGYRLALTAASGQNNQLRIAVSEDGGAPSNT
                     DNTGLSRFVFDETTKQLGEDQKGQDAAFTVNGLSLTRDKNKIDDVVEGFTFQLSKADP
                     GKVVSVTISDDKAFAEEKVRAFVDAYNTFLTAVKPAFAYDQEKQKYGSLAGDSLGKSV
                     LTSVKSLIAQAIPGLSGADMTALTNVGIRTEITDGSLKINETDFKKAFENNFDDVQRL
                     FAPDTRSTNSAITVNSFGKQTTPGSYNVVVTTPPKQGFYTGAALAGSFPLDSTGKTYQ
                     FKIKVNGVESNSITLPADKNYTDSNALISQLQTSINSDAKLLAAGITLTADMSGGAIK
                     LTSARYGSNSTVELTEVHADTAADLGLSVAKGTTGIDVAGTIDGKAAFGFANVLRPDL
                     GEKSEGLNLIVGENATSATVTYTKGFGGELTSLVDKFLLSKGLIDNRKTELNTQLDSF
                     KDDQKDLDRRITAFETRMSRQFRAMESILNGMSGSGKYLDNLFKSLPFTASNN"
     gene            2531..>3198
                     /locus_tag="PKZ52_14990"
     CDS             2531..>3198
                     /locus_tag="PKZ52_14990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNG61127.1"
                     /translation="MPSTRPEQVFEDGVCDACRSAKRKHSAIDWPARRQEFESILNRY
                     RSDGSWYDCIIPVSGGKDSCYQAITLKETYGMNPLCVTFTPCELTVVGRQNLDFLREQ
                     GFDLIQVSHNRKSYAQLQRIGFFKLGDCCWPEHIGIFTAPIRVAVQYQIPLIIWGENS
                     QFEYGGPASRRDNPYLDRSWLEEFQMCGYRISDVVHDGVNLNDIKTFLYPSDDAIRRV
                     GVTG"
BASE COUNT          837 a          720 c          791 g          850 t
ORIGIN      
        1 gctaaatatc ggccattatt ggccgataaa tagacaaagc ccgggcttat acggcaaatg
       61 cttgccgtgg ggcggcaatg ggttgccggt gcaggttgcg tgtcaacggc gtattattaa
      121 ttcggcggct ttttatgcca ggttaatagc cgcgctggat aattgcgctg ttggtgcctg
      181 ttgtgtttag tcgcatggct gcttgtgtgt gaaagtcatt tgagaaggag ttgatgtcgc
      241 ttgatccgct tcctgtggaa gcttgttttt cagattaaat cgtttgtggc gcaggatact
      301 cacctgcatg ctgtcggcgg ttatgcgagg gttttatgat tgacaacaat attgtcaaat
      361 cactgggggc agggtccggt gtggattccg ccaacatcat taaacagctg aaagaaattg
      421 aaaaagctgg ccctcaagcg cgtatcgacg ctaaaaaact taaagcggaa acacaaattt
      481 ctgacttcgg tttaatttcg agttccctga cggctttaaa aaccgccgcc actgcgctta
      541 ctgataaaga gggatttttc agtaaaagtg ctagctatac acccagcgaa gcatttacac
      601 ccgtaaaact ggataccaaa gttgcctcag gcacctatgc atttgatgtc actcagattg
      661 ctaaatccca gtcactttcc agcagcaaat tttcctcact ctccgatgct gtgggcacag
      721 gtacgctcac ctttgaattg ggtagctggg attcaagttc caccaatttc accttggatg
      781 gtacaaaaac cggcaccagc gtcaccatcg atagctccaa caatacatta caaggcctgc
      841 gcgatgcgat taataaagcc gcttttggtg tgcaggcgtc agtggtaaaa gatggcactg
      901 gctatcgctt agcccttacg gcggcgtccg ggcagaacaa tcaattaaga attgccgtga
      961 gtgaagatgg tggtgctccg tcaaatacag acaacacagg tttatcgcgc ttcgtttttg
     1021 acgaaaccac caaacaatta ggggaagacc aaaaaggcca ggatgccgct tttacggtga
     1081 atggtttaag cctgacgcgc gataaaaata aaattgacga tgttgtagaa ggttttacgt
     1141 ttcagttgag taaggccgat ccaggcaaag tagtttctgt caccatcagt gacgacaaag
     1201 cctttgcaga ggaaaaagtg cgcgcctttg tggatgctta taacaccttc ctcacggcgg
     1261 ttaaaccagc ctttgcctat gaccaagaaa aacaaaagta cggctcgctt gcaggggatt
     1321 ccttgggtaa atcagtactc accagtgtta aatcgttaat tgctcaagcg attcccggtt
     1381 taagtggcgc cgatatgacc gcgcttacta atgtgggaat tcgcaccgaa attacagatg
     1441 gctcgttaaa aattaatgag actgacttta aaaaagcctt tgagaataat tttgacgatg
     1501 tacagcgttt atttgcgccg gatacgcgca gcactaatag cgccatcaca gtgaattcgt
     1561 ttgggaaaca aaccacgccc ggttcctata atgtagtggt caccactcca cccaaacagg
     1621 gtttttatac cggggcggca ttggctggca gttttccttt ggattccacc ggcaaaacct
     1681 atcagttcaa aataaaagtc aatggtgttg aatccaattc cattaccttg cctgccgata
     1741 agaattatac cgacagcaat gcactgatta gccagctgca aacatctatt aattcagatg
     1801 cgaaattgct ggcggcggga attacactca ccgctgatat gtcgggtggg gccattaagc
     1861 ttacctctgc ccgttacggt tcaaattcca ccgtcgagct gaccgaagtg cacgccgata
     1921 ccgcagcgga tttgggtttg agtgttgcca agggcaccac aggtattgat gtggcaggca
     1981 ctattgatgg taaagcggca tttgggtttg ccaatgtatt gcggcctgac ttgggagaaa
     2041 aatcggaagg cttaaattta attgtgggtg aaaatgccac cagtgcgaca gtaacctaca
     2101 ccaaaggctt tggtggggag ttaacgtctt tggtggataa attcctatta agtaaggggc
     2161 taattgacaa ccgcaaaacg gaattaaata ctcaattgga ttcctttaaa gacgatcaaa
     2221 aagatttaga ccgccgcatt acggcttttg aaacacgcat gagtaggcag tttcgtgcca
     2281 tggaatcaat tcttaacggt atgagtggct caggcaaata tttggacaac ttatttaagt
     2341 ccttaccctt taccgccagc aataattaag accggggcag ttaaagtctg gagataaggc
     2401 caggctgcct tcggctatcc acctgcggcc cgcttcgccc tggcacttgt cttgctcttt
     2461 cgcctattaa ggaatatttg tcaatttttt gtcaggcggt tgcaccatgc tttattgtag
     2521 tgaatgtatt atgccctcca ctcggcctga gcaggtgttt gaggatgggg tttgtgacgc
     2581 ttgtcgttcc gctaagcgaa aacacagtgc cattgattgg cccgcccggc gtcaagaatt
     2641 tgaatcgatt ttaaatcgct accgcagtga tggcagttgg tatgactgta ttattcccgt
     2701 cagcggcggc aaagattcct gttatcaagc cattacccta aaagaaacct acggcatgaa
     2761 cccgctgtgc gtgaccttca cgccctgtga attgactgta gtggggcggc agaatttaga
     2821 ttttctgcgc gagcagggtt ttgatttaat tcaggtctcc cataaccgta aatcctatgc
     2881 gcagctgcag cgcatcggct tttttaaatt aggtgactgc tgctggccag agcacatagg
     2941 aatttttacc gcacccattc gcgtagcggt gcagtatcaa attccattaa ttatttgggg
     3001 ggagaattcc cagtttgaat acggcggccc tgccagccgc cgagataacc cttatttgga
     3061 tcgcagttgg ctggaagaat ttcaaatgtg tggctatcga atcagcgatg tagtgcacga
     3121 tggcgtaaac cttaacgata ttaaaacctt tttatacccc agcgacgacg caatccgccg
     3181 ggtgggcgtg acgggatt
//
LOCUS       DAOQGS010000481         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17737_length_3196_cov_3.848774, whole genome shotgun sequence.
ACCESSION   DAOQGS010000481 DAOQGS010000000
VERSION     DAOQGS010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17737_length_3196_cov_3.848774"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKZ52_14995"
     CDS             <1..397
                     /locus_tag="PKZ52_14995"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNG61128.1"
                     /translation="PADPSHPHHKREVSTTALQALTLFNSDVVVGWSQALAGRILDDV
                     GTNESDAINRLYEILFARKATSSEKSLLKDFWKKQEKTIEAKAWTDKFEVAVPTGVKE
                     GLKLSPVKAAAFVDLVHTVANSNEFAYRF"
     gene            427..1875
                     /locus_tag="PKZ52_15000"
     CDS             427..1875
                     /locus_tag="PKZ52_15000"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNG61129.1"
                     /translation="MENKFRRDFLINSGLGLGGAALSGFVPGLGTVGAAFAEDLIDPL
                     APKAPHFPAKIKSVIWLHQNGAPSTLDLFDYKPELVKLAGTEVPPSFLKGIKTSTQGG
                     VGKLFVSKDRTWKQYGESGAWFSNLVPNIAQHADKLTFIKSSMTVGATHDISILKLNT
                     GDLNPGRPSLGAWISYALGSANPDLPPYVVLYNGKSEPRAGSVNWNSGFLPAVYQGTA
                     FRPGPSPIYYLDRPDLRTSDQQRNALGLLKKLNEKNGDRYPSDSELKARLRSYELADR
                     MQIAAPEAVDISKETAATKELYGLNDSHTAGYGNTLLRARRLVERGVRFIQVVTGEPE
                     GETEITSWDAHDSLEKNHSTMAKMVDKPIAGLLADLQSRGLLESTLVVWTSEFGRTSW
                     GESGTGRDHNAWGYTQWLAGGGLKAGFTYGATDEIGLQVADKETAVDTYDLHATVLQL
                     LGLNHLKTTFLNNGRSERPTVVYGKVIKDILA"
     gene            1926..2309
                     /locus_tag="PKZ52_15005"
     CDS             1926..2309
                     /locus_tag="PKZ52_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61130.1"
                     /translation="MYRLSTTLIILLSLYVTSVHAAEAEIDTDLMQNIEDTNKSLSSN
                     IALENGKGSASDAKDLSEMFAVVEQHFAKKPGATDATNLSKKSKDLSLQIIVDVGAKR
                     FEEANNLATELSRACKSCHAFYKKD"
     gene            2312..2998
                     /locus_tag="PKZ52_15010"
     CDS             2312..2998
                     /locus_tag="PKZ52_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNG61131.1"
                     /translation="MPALLSRGVSHGIVFLALYAIDAAAAGSSTQKTTSSQEETWQAD
                     SRAGKILAEENRCAECHGLDGNGQSNTAGSLGVFPKLAGQNAAYLWKQIKDFRSDQRH
                     SDIMSIMARSVDDADARNILGYYSSLPKMQGKAAASNPAAESLFKRGDDKRGLAACAR
                     CHGEGGKGLAAQPLVPIIGGQDRAYLHKQLSDWRSGERKNSPDGAMSAAAKLLTDQEI
                     NSLADYLSAL"
BASE COUNT          817 a          758 c          809 g          812 t
ORIGIN      
        1 cccggccgat ccatcccatc cgcaccacaa gcgcgaggtt tccaccacgg cgctgcaagc
       61 cctcacttta tttaatagtg atgtggtagt gggctggtcc caggccttag cgggtcgcat
      121 tttggatgat gtaggcacca acgagagcga tgcgattaac cgtttgtacg aaatattatt
      181 tgcccgcaag gcaacctctt cagaaaagtc tttgctcaaa gatttttgga agaaacagga
      241 aaaaactatt gaagctaaag cctggacgga taaatttgaa gtggcggttc ccacgggagt
      301 gaaagaaggc ctgaaactct cgccggtcaa agctgctgcg tttgttgacc tagtgcatac
      361 ggttgccaat tccaacgaat ttgcctaccg cttttaaacc ccatttacta atacgagatt
      421 aagactatgg aaaacaaatt tagacgcgat tttttaatta actccggttt aggcttaggc
      481 ggcgctgcgt taagtggatt tgtccctggc ctcggcactg taggtgctgc ttttgcagag
      541 gatcttatcg accctttagc acctaaagcg ccccatttcc ctgcgaaaat taaatcggtg
      601 atttggctgc atcaaaacgg cgcgcctagc accttggatc tatttgatta caagccagaa
      661 ctggtgaagt tggcgggtac tgaagtgccg ccttctttct taaaaggtat taaaaccagt
      721 acccaaggtg gcgtgggcaa actttttgtg tccaaagatc gcacctggaa acaatacggt
      781 gaaagcggcg cttggttttc taacctagtg cccaatatcg ctcagcatgc ggataaactt
      841 accttcatta aatccagcat gaccgtgggt gccacccacg atatttcgat tttaaaactc
      901 aataccggtg atcttaaccc tggtcgtcct tctttaggtg cttggatttc ttacgcatta
      961 ggttctgcta atcctgattt gccgccctat gtggtgttgt acaacggtaa gtctgagcct
     1021 cgcgccggtt ctgtaaactg gaactcaggc tttttaccgg ctgtgtacca aggcactgcc
     1081 ttccgccccg gcccgtcgcc aatttattat ttggatcgcc ctgatctgcg cacttcagat
     1141 caacagcgta acgccctcgg tttattgaaa aaactcaatg agaaaaatgg cgaccgctac
     1201 ccgtcagact ccgaattaaa agcgcgcttg cgctcttatg aattggctga tcgcatgcag
     1261 attgccgcgc ctgaagcggt ggatatttct aaggaaaccg ctgcgactaa agagttatac
     1321 ggcctgaatg attcgcacac agcaggttat ggcaatacat tattgcgtgc ccgccgttta
     1381 gttgaacgcg gcgtgcgttt tatccaggta gttaccggtg agccagaagg cgaaaccgaa
     1441 attaccagtt gggatgccca cgatagcttg gagaaaaacc acagcaccat ggcaaaaatg
     1501 gtggataaac ccattgccgg tttgttagcg gatttacagt cgcgcggttt attggaatca
     1561 actttggtgg tgtggacttc cgaatttggc cgcacctctt ggggtgagag cggaacaggc
     1621 cgcgaccaca atgcctgggg ttacacccaa tggttggcag gtggcggcct gaaggctggc
     1681 ttcacttacg gcgctactga tgaaattggt ttgcaggttg ccgacaaaga gacagctgtg
     1741 gatacctatg atttacatgc cacggtattg caattgctcg gccttaacca ccttaaaaca
     1801 accttcttaa ataacggtcg ttcagagcgg ccaactgttg tatacggcaa ggtgattaaa
     1861 gatattttgg cttaattgtt cccgggcagg gatgcccgca ttttttctgc gagcttcgtt
     1921 ctaatatgta ccgcttgagc accaccctaa ttatcctttt atctttatac gtaacctccg
     1981 tgcatgcagc tgaggctgag atagatacgg atttaatgca gaatatcgaa gataccaaca
     2041 agagtttatc ttccaatatc gcgctggaaa acggcaaagg ctcggcgtca gacgctaaag
     2101 acctatcgga gatgtttgcc gtggttgagc agcattttgc caaaaaaccc ggtgccaccg
     2161 acgccactaa cctatctaaa aaaagtaaag atttaagcct gcagataatt gtcgatgtgg
     2221 gggctaaaag atttgaagag gccaataatc tggctaccga attgtcgcgg gcttgtaagt
     2281 catgccacgc attttataaa aaggattaag ggtgccagca ctattaagcc gtggggtatc
     2341 ccacgggatt gtttttttag ccctatatgc aattgatgca gccgccgcag gctcatccac
     2401 tcaaaagact acatccagcc aagaggaaac atggcaagcc gatagccgcg caggcaaaat
     2461 acttgctgaa gaaaatcgtt gtgccgaatg ccacggcctt gatggtaatg gccagagtaa
     2521 cacggcgggt tctttagggg tatttcccaa attggccggc caaaatgcgg cttatttgtg
     2581 gaagcaaata aaggattttc gcagcgacca gcgccatagc gatattatgt ccatcatggc
     2641 gcgctcagtt gatgatgcgg atgcccgcaa tattctaggt tattattctt cgttgcctaa
     2701 aatgcagggc aaagccgccg cgtcaaaccc tgccgccgaa agtttattta aacgcggtga
     2761 tgataagcgc ggacttgcgg cctgtgccag gtgccacggg gagggaggca aaggtttggc
     2821 ggcgcagccg ttagtgccca ttattggtgg ccaagatagg gcctacttgc acaaacaatt
     2881 atcggactgg cgcagcggcg agcgcaaaaa tagcccggat ggggctatga gtgccgcagc
     2941 caaactctta accgaccagg aaattaacag cttggctgat tatttatcgg cactttagcg
     3001 cgtttggtta cattggtgtt cgcccctgtg cagacacacc ttttattgat ttgatcattt
     3061 cacgagagaa tatttatgcg actagtacaa ataggcctgg cgttggcggc attgagcagt
     3121 ttgcctgcgc aagcggcgtt acaagtgggc gataaagcgc cagactttaa attgactgcc
     3181 gccctagcgg gtaaag
//
LOCUS       DAOQGS010000482         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17767_length_3192_cov_4.807459, whole genome shotgun sequence.
ACCESSION   DAOQGS010000482 DAOQGS010000000
VERSION     DAOQGS010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17767_length_3192_cov_4.807459"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKZ52_15015"
     CDS             complement(<1..546)
                     /locus_tag="PKZ52_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNG61132.1"
                     /translation="MIPQRAFFSSLLLILSGCLALASMQAFAADPAATYLDSQQSFAL
                     RAQDLVSRMSLQEKIAQLQNDAPAIERLQVPKYEWWNEALHGVARAGGATVFPQAIGL
                     AATFEPEFMHTVASAISDEGRIKYRLFSSQGERNRYQGLTFWSPNVNIFRDPRWGRGQ
                     ETYGEDPYLTSRMGVAFVKGLQ"
     gene            complement(595..918)
                     /locus_tag="PKZ52_15020"
     CDS             complement(595..918)
                     /locus_tag="PKZ52_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61133.1"
                     /translation="MKTLELIRAPEAQLVLALKEMKLKELEKHGQNLVRLLGGEDYRL
                     VLRSVIKVLPSLSQEPAARFEALNQALAPLLQKAAEQPDLLAKLSIILTLVVRLKFTA
                     IHENR"
     gene            1631..3085
                     /locus_tag="PKZ52_15025"
     CDS             1631..3085
                     /locus_tag="PKZ52_15025"
                     /inference="COORDINATES: protein motif:HMM:NF016224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HNG61134.1"
                     /translation="MSKPWDKQRLKHTLARAKGQLKQEASRVKGQVAVNLARLLERRV
                     VVGITGFSGAGKSTLITSLINQLLQFQTASIPGFSPALSNRIRWVKYHPAATGFERNF
                     NYQYFYKHLACEDNQWPASTVDISAATLEIGLSRGPGSGWLAPKEYSLWVELRDYPGE
                     WLMDLLLADMSFIDWCAWCQARFDRYSHLPDIQALRETDPIKAADPQRLIYLVERYEA
                     LAKSVFNQAQDLRFVLPGRVLIPQAFNLSKLPFVPLFLRGHSKAELINAPADSYFCVL
                     QANFERYKTQWVKPFVEKVFGGLDRQLILVDVLQALHQGPEFTEGLVDALAGISESFK
                     YGKTRPWLQWMLPKIDKVVFAATKIDQIVASEHEAVRQLLGVLVKKAYTQTQQAGAAP
                     AIEAVAAVRASVEQEYAGQAGIVGLGQAGKPVGFIHPSVPAQWPSPAHWQAFGQWQPP
                     LLLPPKGLDYRNHDALPHIRMDSLINSLLGDFCP"
BASE COUNT          683 a          838 c          932 g          739 t
ORIGIN      
        1 ctgcaggcct tttacaaatg caacccccat gcggctggtt aaataggggt cttcgccgta
       61 ggtttcctgg ccgcggcccc agcgcggatc gcgaaaaata ttcacattcg gcgaccaaaa
      121 ggtgaggccc tggtagcggt tgcgctcgcc ttggctgctg aataaacggt atttaatgcg
      181 gccttcatcg ctgatggcgc tggcaacggt gtgcatgaat tctggctcga aggtggcggc
      241 aaggccgatg gcctggggga acaccgtggc gccgcctgca cgggctacgc cgtgtagggc
      301 ctcattccac cattcatatt taggcacctg caggcgctcg atggcggggg cgtcgttctg
      361 cagctgggcg attttttcct gcaggctcat gcgggagacc aaatcctggg cgcgcagggc
      421 aaaactctgt tggctatcta aatacgtggc tgcggggtca gccgcaaaag cttgcatgga
      481 agcaagggca aggcagccgc ttaggatgag aagcaaggag gagaaaaaag cgcgttgggg
      541 gatcatgggg aggctcttgt cggaatgcgc tgagcatgcg cacagagggt gggcttaacg
      601 attctcgtga atagcggtga atttaagccg caccaccagg gttaaaataa tgcttaattt
      661 ggcgagtaaa tcaggttgct cagcggcttt ttgcagcagc ggggcgaggg cttgattgag
      721 tgcttcaaaa cgcgcggcag gttcttggct gaggctgggc agcactttaa taacgctgcg
      781 cagcaccagg cgataatcct cgccaccgag cagccgcacc agattttgcc cgtgtttttc
      841 cagctctttt aatttcattt ctttgagggc gagtaccagc tgggcttccg gggcgcggat
      901 taattcgagg gttttcataa gggcgtctta aaaaggaaag cagggtagcg gcttataaat
      961 acttcggcaa atgattctgc ctggggggta agttgtgcag tggctttatc tgattgcttt
     1021 gtattaactt ggcggcaaaa tagatcgtcc tgatctattt tgccgtcgct gcgttaaaac
     1081 ttacgaaaat gcgtggattt tcttgtttta acgcagctcg cggcggtttg caccgccgag
     1141 cgcggcatcc ttgccgcgat cattaacccg gcgctaagtg ccgccgggtt aataactatt
     1201 cagcatcttt ttgtgtggcg ggtttgtggc tggaggtaat tcccgcactg actgatagct
     1261 atcgcggacc tgtgttagtg ctgtaacccc gcaatgcccc tccgcagtgg tgaccatagg
     1321 ttagtgcaga cacgccgtga atacatccgt gtaggctcgt caaaaagatc cctctttttg
     1381 acggtctgca ctaacctatg gtcacaactg ctataccgcg aatcgatggt gccggttggc
     1441 taaatacccc tcaatttccc gccctcacgg atgataaggt gttctgaata cgtacttgct
     1501 ttgccgcaat tacacttggg cagcggcagt tggcgatgca acatccgggt tataaacccc
     1561 ctttaagttg acattgcagc taaatttagc cagcgttggg ccaccacaac acagctggat
     1621 ctactggcct atgagcaaac cctgggataa acagcgcctt aaacacactc ttgcccgcgc
     1681 caaggggcag ttaaaacagg aggcgagccg cgtgaaaggg caggtggcgg taaatttggc
     1741 gcgcttgctc gagcggcggg tggtggtggg cattaccggc ttttccggcg ccggtaaatc
     1801 caccctgatt accagcctta tcaatcaact actgcagttt caaaccgcca gcatcccagg
     1861 ctttagccca gccctgagca accgcatccg ctgggtgaag taccaccccg ctgccaccgg
     1921 ctttgagcgc aattttaatt atcagtactt ttataaacac ttagcctgtg aggacaacca
     1981 gtggcccgcc tccaccgtgg atatcagcgc cgctacttta gaaataggcc ttagccgcgg
     2041 gccgggcagc gggtggctcg cgcccaagga gtacagcctg tgggtggagc tgcgggatta
     2101 ccccggcgag tggctgatgg atttattgct ggcggatatg agttttatcg actggtgcgc
     2161 ctggtgccag gcgcgttttg accgctacag ccacctgccg gatatacagg ctcttaggga
     2221 gaccgacccc attaaagctg ccgaccccca gcggcttatt tacctggtgg agcgctatga
     2281 ggccctggcc aaaagcgtgt ttaaccaagc tcaggattta cgttttgtgc tgccgggacg
     2341 ggtgttaatt ccccaggcgt ttaatttaag caaactgcct tttgtgcctt tatttttgcg
     2401 cggccacagc aaagccgaat taatcaacgc ccctgcggat tcttattttt gcgtgttgca
     2461 ggccaatttc gaacgctaca aaacccagtg ggtaaaaccc tttgtggaaa aggtattcgg
     2521 cgggctggat cggcaattaa ttttggtgga cgtattgcaa gccctgcacc aggggcctga
     2581 atttaccgaa ggcctggtgg atgccctggc gggtattagc gagagtttta aatacggtaa
     2641 aacccgcccc tggctgcagt ggatgctgcc gaaaatcgac aaggtggtat ttgccgctac
     2701 caaaatcgac cagattgtgg ccagtgaaca cgaggcggtg cgtcaactgt tgggggtatt
     2761 agtaaaaaaa gcctacaccc aaactcagca ggcgggcgcc gctcccgcca ttgaagcggt
     2821 ggccgccgtg cgcgcctcgg tggagcagga gtacgcaggc caggcgggca tagtgggcct
     2881 ggggcaggcg ggtaaaccgg tgggctttat ccacccgagt gtgcccgccc agtggccaag
     2941 cccggcccat tggcaggcct ttggtcagtg gcagccgccc ctgttattgc cgcctaaagg
     3001 ccttgactac cgcaaccacg acgccctgcc gcatattcgt atggacagtt taatcaacag
     3061 tttattgggg gatttttgcc cgtgagtctt gagccgaaag ttgaactgaa caaacccgcc
     3121 tttgagtcgc ccttgcgcca agcgcaactc tttaccccgg aggaggccgc gccggagccg
     3181 gtgcgcacgg ct
//
LOCUS       DAOQGS010000483         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17824_length_3187_cov_4.855683, whole genome shotgun sequence.
ACCESSION   DAOQGS010000483 DAOQGS010000000
VERSION     DAOQGS010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17824_length_3187_cov_4.855683"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1197
                     /locus_tag="PKZ52_15030"
     CDS             43..1197
                     /locus_tag="PKZ52_15030"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNG61135.1"
                     /translation="MNNFADLNAVLGQEHGDRLLIAVAERLKNRFKPISQVARIGADI
                     FAIFGPASHVNETRLLAPFGNPFEIAGELQLIGISCGIAPVSNDIASARELLKYANIV
                     LKDLKQKQAQEKVAYFNPTMMDEAKKRLHLLKELRHAFDRDELFLAFQPKIALHNRQV
                     TGFEALIRWRNSRGQFVSPLDFIPLAEQSGLIVRLGEWVLSQAMRSLAQIRAHGFHGV
                     SMAVNVSVVQLQHPEFLHMLKRVIGETLIDASAIELEVTESVEMGNIQNHIQQLQSIK
                     DLGFSLAIDDFGTGFSSLSYLQQMPIDCLKIDRSFIQDVTQCAGANIVQMVLQLARSL
                     RLTVVAEGAETIEQVEALEALGCDLVQGYYFAKPMPLDDLLLWLAAYRSS"
     gene            1419..1495
                     /locus_tag="PKZ52_15035"
     tRNA            1419..1495
                     /locus_tag="PKZ52_15035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Arg,seq:acg)
     gene            complement(1642..2844)
                     /locus_tag="PKZ52_15040"
     CDS             complement(1642..2844)
                     /locus_tag="PKZ52_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG61136.1"
                     /translation="MERVLIIDDDRFTQNVLQKSLYKHYETRTADNGAVGLSLAQNWH
                     PDAILLDVEMPGQNGFEVCDVLKRDKATQEIPVIFLSSRSSIRERMLGFEVGADDYLT
                     KPCSQEMLTAKLQKIIALYHQRNELRDTIKTAEQTALEAMATSFELGKAIRFVEKSYN
                     YSTVERLGAALIEVMESLDLNACVRLNSRFATITLATGGDSVKPLEQDLMAMLHTEQR
                     FVDFGCRSQINYPGVALLVKNMPLDNRARYGRLKDTLPFVLGACDAKLRMLDAEHALM
                     LQNRELSNSVLSVQRTLSDVSQLLVKNQRAIGAVMSDLTTELSVHMNKLGLEGDQEEF
                     IHHKVDVAANKLYSCVAEGNVVENILSGIAELLRQMSSEQARIISETLSTQMANQDES
                     ITSDVELF"
     gene            complement(2980..>3187)
                     /locus_tag="PKZ52_15045"
     CDS             complement(2980..>3187)
                     /locus_tag="PKZ52_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61137.1"
                     /translation="HAVNEHFFAKFNDARASELIFQRFPRGEYCHELLPLPMYSSALR
                     QCSPFISIGLNGDMGITTTLKTID"
BASE COUNT          798 a          821 c          761 g          807 t
ORIGIN      
        1 gcaccctaaa ggcagaaaac cttatttaca agattaattg atattaataa ttttgccgac
       61 ctgaacgcag tgctgggcca ggagcatggg gatagactgt taattgccgt tgccgagcgc
      121 ttaaaaaatc gttttaaacc catcagccag gtggcgcgca tcggtgcgga tattttcgct
      181 atttttggcc cggcgtccca tgttaacgag acgcgcctgc tcgcgccctt tggcaacccg
      241 tttgaaattg ccggtgaact gcaattaatt ggtattagct gcggtattgc gccggtatca
      301 aatgatattg cctccgcccg agagctgctt aaatacgcca atattgtgct taaagacctc
      361 aagcaaaagc aggcgcagga aaaggttgcg tactttaacc ccaccatgat ggacgaggcg
      421 aaaaaacgcc tgcatttatt aaaagaattg cgccacgcgt ttgatcggga tgaattattt
      481 ttagcctttc aacctaaaat tgctttgcac aaccgccagg taaccggttt tgaagcctta
      541 attcgctggc gcaatagccg cggccaattt gtctccccgc tggactttat tcctctggcc
      601 gaacaaagcg gtttaattgt gcgtttaggt gaatgggttt taagccaagc catgcgcagc
      661 ctggcccaga tacgtgcaca cgggtttcat ggcgtgagca tggcagtgaa cgtatccgtg
      721 gtgcagttac aacaccctga atttctccac atgctcaagc gggtaatcgg tgaaaccctc
      781 atcgacgcca gcgctattga attggaagtt accgaatcgg tggaaatggg caatattcaa
      841 aatcatattc agcagctaca aagcattaaa gacctggggt ttagcctagc cattgacgat
      901 tttggcaccg gcttttcatc cctaagttac ctgcaacaaa tgccgattga ttgcttaaaa
      961 attgatagaa gttttattca agatgtcacc cagtgcgcgg gtgccaatat agttcagatg
     1021 gtgctgcaac tggcccgctc cctgcgctta accgtggtgg ccgaaggggc ggaaaccatt
     1081 gaacaggtcg aggcactgga agccttgggc tgcgatctgg tgcagggcta ttattttgcc
     1141 aaacccatgc ccttggatga cctgctgctg tggcttgcag cctataggtc gagttaaaag
     1201 gcggcagttt agaataaccc ggccttgcgc tgcggggcct caaagcgata aaacaggaag
     1261 ctcagaggcc attgcttggg cgccttcgcg ctcaactcca atggatttcg ggccagggcg
     1321 ccaagtagtt aaatttaatg aagaaaactg ttgacgaggg cgagtcaaag ccataacatg
     1381 cgcgccctca gctggtgaca ctgatcacta ctgaagaagc actcgtagct cagctggata
     1441 gagtactcgg ctacgaaccg agcggtcaga ggttcgaatc ctctcgagtg cgccatatac
     1501 cgccccaccg gggcaataaa aaagcctgct taatgcaggc tttttttatg cgctacactt
     1561 ttattttttg tacgcgcatt taatccctcg agggttgcat tattaacccg acattaaatg
     1621 gcgccgtttg aataattgac actaaaacaa ttccacatcg ctggtaatgc tctcatcctg
     1681 gtttgccatt tgggtggaga gggtttcgga gataatgcgc gcctgctcgc tgctcatctg
     1741 gcgcaataat tcggcaatcc cactgagaat attctccacc acattgccct cggccacgca
     1801 gctgtacaat ttattcgctg ccacatccac tttatgatga ataaattcct cctgatcccc
     1861 ttccaggccg agcttattca tatgcacact cagctccgta gttaaatcgc tcatcaccgc
     1921 gccgattgcc cgctggtttt tcaccaatag ctgcgacacg tcagaaagtg tgcgctgcac
     1981 actgagcacg gaattggata attcacggtt ttgcagcatc aaggcgtgct cggcatcgag
     2041 catgcgcaac ttagcatcac aagcgcccaa tacaaacggc agcgtatctt ttaagcggcc
     2101 atagcgggcg cgattatcca gcggcatatt ttttactaac agcgcaacgc cgggataatt
     2161 aatttgcgag cgacaaccaa aatccacaaa gcgctgctcc gtgtgcaaca tggccattag
     2221 atcttgctca aggggtttta cgctatcccc tccggtggca agggtaattg ttgcaaatcg
     2281 gctatttagc cgcacgcagg cattcagatc caaactttcc atgacctcaa tcagcgcagc
     2341 ccccaggcgc tcaacggtgc tgtagttata ggatttttcc acaaagcgta tggctttacc
     2401 caattcaaaa ctggtggcca tggcttctaa ggccgtttgt tcagcggttt ttatggtatc
     2461 gcgcagctcg ttgcgctgat ggtagagcgc gattattttt tgcagcttgg cggtgagcat
     2521 ctcttggctg cagggcttag ttaaataatc atccgccccc acctcaaaac ccaacatgcg
     2581 ctcgcggata ctggaacggc tagataaaaa tatcaccggg atttcttggg tggctttatc
     2641 gcgtttgagc acatcgcaca cttcaaagcc gttttggccg ggcatttcca catccaataa
     2701 aatggcatcc gggtgccagt tttgcgccaa actcaaaccc accgcgccgt tatcggcggt
     2761 gcgggtttca taatgtttat acagcgattt ttgcaggaca ttttgcgtaa atcggtcgtc
     2821 gtcgatgatt agtactcgct ccattactcc acaccacgct tacacttaaa gacactaagc
     2881 acgcaggctt gtcaccggcg tattacttgg cagttaaatc cgctccccga gacttggcag
     2941 ggcatctaca caatcccctg gcctgcccgg ccccagcgtt taatcaatcg ttttaagtgt
     3001 agttgtaatt cccatatcgc cattaaggcc aatcgaaata aaaggcgagc attggcgcaa
     3061 agcagaggaa tacatcggca gcggcaacaa ctcatggcaa tactcgcctc tagggaacct
     3121 ctgaaaaatt aactcactcg ccctagcgtc gttgaatttg gcaaaaaaat gctcatttac
     3181 tgcgtgt
//
LOCUS       DAOQGS010000484         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_17871_length_3182_cov_3.924848, whole genome shotgun sequence.
ACCESSION   DAOQGS010000484 DAOQGS010000000
VERSION     DAOQGS010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_3182_cov_3.924848"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKZ52_15050"
     CDS             <1..782
                     /locus_tag="PKZ52_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNG61138.1"
                     /translation="TLLAGILRQNPAIHAAMTSPVAGLIQGALEQMGANSEAYSFFDD
                     EKRKTICKALFSGYYADKTQPIIFDTNRHWTARLHQLVPLFSDFKMICCVRNPAWIMD
                     SFEVIYRKNPFDFSRMFSPDSRQTVYTRCESLAHTHGPLGLAWTALKEAFYGEFSERL
                     LLVDYDLLTQHPAKTLDLIYQFIGEPVFDHDFNQVVYEESEFDQQLGVKGLHSVGRKV
                     EFKSRRSILPPDLFLKYQEMDFWQDTNGSAANIIASKKPTL"
     gene            807..>3182
                     /locus_tag="PKZ52_15055"
     CDS             807..>3182
                     /locus_tag="PKZ52_15055"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNG61139.1"
                     /translation="MKNKLLCGLSSAFFPLGLYAAAGQLQNQYQRRSLSAQVAPAALE
                     FDQVLPVAATHRYKTPLTPVGKENSGLEIIASTPVRELLVLDGAVPNKEVFYRSIKPG
                     VAIAEINSTAPGLPQLIAILQQYKNLAAVHLVSHAEAGVLLLGNSRINAESFKADIQA
                     FAAIDNAMAEGADLLLYGCNLAADAAGGDILRLIKNNTHVDVAASNNATGAANLGGDW
                     QLEIQQGRVDAGLPLNERALKDFTAILGTYTPRNFCNSSTGGNYQGPITSRNSQDGNY
                     RVTTSPSNFFCGYNGTLGYLSGNHSIYLTKPSGGTFTLTAISLGGGCSTVSAYAKNGV
                     TTTASSLNNATAAYNNHINMTNLTGVSMDKLQLKLTGCAGSMGISSFSVPDANSAPTN
                     ISLSASTVNQSASANATVGTLSSTDPDAGNTFTYSLVSGSGSTDNASFNISGSSLRAT
                     SPSSLSAGSKSVRVRTTDQGSLTFEKAFTITVVDNVAPTVSSVNSSTANGTYKVGDAV
                     SVQVNFSEAVTVTGTPQLTLETGTTDRTLNYASGSGTTALTFTYTVQAGDVSADLDYT
                     ATSALALNGGTIRDGASNNATLTLASPGAANSLGANKALVIDGVAPTVSSVGSTTSNG
                     SYKTGDVISVQVNFSEAVTVTGTPQLTLETGSTDRAVNYTSGSGTSSLSFNYTVQAGD
                     TSADLDYLSTSALALNGGTILDAAGNAATLTLAAPGAANSLGNGKALVVDTSGPTATI
                     VVADTALKIGDTSLVTFTFNEAVSGFTNADLTIANGTLSSVSSSDGGITWTA"
BASE COUNT          748 a          885 c          841 g          708 t
ORIGIN      
        1 ctaccttgct ggcggggatt ttgcgccaga accctgctat tcatgccgct atgacaagcc
       61 cggtggccgg tttaattcag ggcgctttag agcaaatggg ggccaatagt gaggcctata
      121 gtttttttga cgacgaaaaa cgcaaaacca tttgcaaagc gctatttagc ggctactacg
      181 ccgataaaac ccaaccgatt atttttgata ccaaccgcca ttggacggcg cgcctgcatc
      241 aactggtgcc cctgttcagc gattttaaaa tgatctgctg cgtgcgcaat ccggcctgga
      301 ttatggatag tttcgaggtt atttaccgca aaaacccctt tgatttcagc cgcatgttct
      361 caccggactc ccgccagacg gtgtataccc gctgtgaatc cctagcccat acccacgggc
      421 ctttgggcct tgcctggacg gcgctgaaag aggcgtttta tggggagttt tccgagcgct
      481 tattgttggt ggattacgac ctgctcaccc agcatccggc taaaaccctg gatttaattt
      541 accagtttat cggtgagccg gtgtttgatc acgactttaa ccaggttgta tatgaagaaa
      601 gtgaatttga ccagcagctg ggcgtaaaag gcctgcacag cgtaggccgc aaggttgaat
      661 tcaaatcccg ccgcagcatt ttgccgccgg atttattttt aaaatatcag gagatggatt
      721 tttggcagga taccaatggc agtgccgcca atattatcgc cagcaaaaaa cccactcttt
      781 aagctgatta cacggactac gaatctatga aaaataaact actgtgtggt ttgtcatctg
      841 catttttccc ccttggattg tacgcagctg ctggtcaact gcagaaccag taccagcgcc
      901 gcagcctcag cgcgcaggtg gcgccagcgg cgcttgaatt tgatcaggtg ctgccggtag
      961 cggcaacgca cagatacaaa acgcccttaa cacctgtagg taaagagaat tctgggctgg
     1021 aaattattgc cagtaccccg gtgcgggaat tattggtgtt ggatggcgcg gtgccaaata
     1081 aagaggtgtt ttaccgcagt attaaacccg gcgttgccat tgccgaaatt aattccaccg
     1141 ccccaggcct gccgcaatta attgctattt tgcagcagta taaaaatctt gccgccgtgc
     1201 atttagtatc ccatgccgaa gccggtgtgt tgttgctggg taattcgcgc attaatgcag
     1261 agagtttcaa agcggatata caagcctttg cggcaattga taatgccatg gcggagggcg
     1321 cggatttatt gttgtacggc tgtaatttag cggccgatgc agcgggcggc gatatactca
     1381 ggctgattaa aaacaatacc catgtggatg tggccgcctc caataatgca acgggagcgg
     1441 cgaatttagg tggcgattgg cagttggaaa ttcagcaggg cagggtggat gcagggctgc
     1501 cgttaaatga gcgggcgctg aaggatttta cggctatttt aggtacttat acgccgcgca
     1561 atttttgtaa tagcagcacg ggcggcaatt atcagggccc tattaccagc cgaaattccc
     1621 aggacggtaa ttaccgcgtt actaccagcc ccagtaattt cttttgtggt tacaacggca
     1681 ccttaggtta tctatccggt aaccacagta tctatctcac caagcccagc ggcggcacct
     1741 ttacccttac ggctattagc ctagggggcg gctgttctac ggtgagcgcc tatgccaaaa
     1801 acggcgttac cacaacagcg tcatcgttaa ataacgctac tgcggcttat aacaaccaca
     1861 tcaatatgac caacttaacc ggcgtcagca tggataagtt gcagttgaag ttgaccggtt
     1921 gcgcagggtc catgggtatc agtagtttct ccgttcccga cgccaacagc gcccccacca
     1981 atatcagcct gtcggcctcc acggtaaacc aaagcgcaag tgctaatgcc actgtaggca
     2041 ccctgagcag taccgacccg gatgcaggca acacctttac ctacagcttg gtatccggca
     2101 gcggcagcac ggataacgcc agtttcaata tcagcggttc cagcctcagg gccaccagcc
     2161 cctcctcctt gagtgccggg tctaagtcgg tgcgggtgcg caccacggac cagggcagcc
     2221 tgacctttga gaaggccttt accatcaccg tggtggataa cgtcgcgccc accgtcagct
     2281 cggttaattc ctccaccgct aatggcacct ataaggtggg ggatgcggtg agtgtgcagg
     2341 tgaattttag cgaggcggta accgtaaccg gcacgcccca gcttacgctt gagaccggca
     2401 ccaccgaccg caccctgaac tacgccagcg gcagcggcac caccgccctc acctttacct
     2461 atacagtaca agcgggggat gtctcagcgg atttggacta caccgcaacc agcgccctgg
     2521 ccttaaacgg cggcaccatc cgcgatggcg ccagcaacaa tgccaccctc accctggcca
     2581 gccccggcgc ggccaactcc ttaggcgcca acaaagccct ggtgattgat ggggtggcgc
     2641 cgacggtaag ctcggtgggt tccaccacca gtaacggcag ctataaaacc ggcgatgtaa
     2701 ttagcgtgca ggtgaatttt agcgaggcgg taacggttac cggcacaccg cagcttacgc
     2761 ttgagaccgg cagtacggac cgggccgtca actacaccag tggcagtggc accagcagcc
     2821 tcagctttaa ctacacggtg caagcggggg ataccagcgc cgatttggat tacctctcaa
     2881 ccagcgccct ggccctaaac ggcggcacta ttttggatgc ggcgggcaac gccgctaccc
     2941 tgaccctggc cgcccctgga gctgccaact ccttgggcaa tggcaaggct ctggtggtgg
     3001 ataccagcgg ccccaccgca accattgtgg tggcggatac agccttaaaa ataggcgata
     3061 ccagcctagt caccttcacc tttaacgagg cggtaagcgg ctttaccaat gcggatttaa
     3121 ccatcgccaa cggcacccta agctccgtca gctctagcga tggcggcatt acctggacag
     3181 cg
//
LOCUS       DAOQGS010000485         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18178_length_3148_cov_3.716457, whole genome shotgun sequence.
ACCESSION   DAOQGS010000485 DAOQGS010000000
VERSION     DAOQGS010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18178_length_3148_cov_3.716457"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="ribF"
                     /locus_tag="PKZ52_15060"
     CDS             <1..786
                     /gene="ribF"
                     /locus_tag="PKZ52_15060"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNG61140.1"
                     /translation="VVIFEPQPQEYFNAEAAPARLFGLRDKVLALKALGVDQVLCLRF
                     NRWLRNLTAEEFVRQVLVDWLDVRHLEVGDDFRFGRDRAGDYAFLTRAAITYGFTLAN
                     NPTLVRLGERVSSTRIRTLLSQGELAAAADLLGQPYSISGRVVHGRALARTLGFPTAN
                     IAIGNYRRALAGVYAVEVEGPCGCLPAVANIGVKPTIADGLKPSLEVFILNGSYELYG
                     ARLRVRFLRWIRAEQKFINIEALKQQIQRDVKAAADFFTTRQT"
     gene            829..>3148
                     /gene="ileS"
                     /locus_tag="PKZ52_15065"
     CDS             829..>3148
                     /gene="ileS"
                     /locus_tag="PKZ52_15065"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNG61141.1"
                     /translation="MTDYKATLNLPATTFAMKANLAQREPEMLKRWQQEGIYQQIRAA
                     RAGCPTFILHDGPPYANGDIHIGHAVNKILKDIIIKAKTLSGFDAPYIPGWDCHGLPI
                     EHNVEKKIGKAGVKVDVATFRRHCREYASKQVAGQKADFIRLGVLGEWDKPYLTMDYK
                     TEADTVRALGKIIANGHLQRGFKPVYWSVVGASALADAEVEYHEKTSFSIDVRFAVEE
                     DALLRKIGAIKGTGPISVVIWTTTPWTLPANQAVSLNAELDYALVQLNAERIIVAKAL
                     IEALCARSKIAEFTIVQEFKGADLEHLLLQHPFYPKHVPIILGDHVTTDAGTGAVHTA
                     PDHGMEDFVVGKKYNIGTLNYVDENGNYSAQTPIFAGEHVYKADEKVVALLEERGALF
                     HQGKITHSFPHCWRTKTPLIYRATPQWFISMTQNNLLEQVKTSVGEVKWFPTWGESRM
                     RGTLESSPDWCISRQRTWGVPITLFIHKETQIPHPNTVELIDQVAQLIEQKGMDAWFE
                     LDAAELLGDEASHYTKVTDTLDVWFDSGVTHYSVLEQRPEVSFPADLYLEGSDQHRGW
                     FQSSLKTSVAMNGCAPYKQVLTHGFAVDEKGLKMSKSLGNVVPPQKVVNDLGADVLRL
                     WVAATDFSGDMSVSDTILNRTADSYRRIRNTVRYLLSNLPDFNPATDLIALDDMVALD
                     RWAVARAAALQGEIISHYDAYDLHLIYQKLHNFCVVDLGGFYLDITKDRVYTCAQNSA
                     ARRSAQSAQYHIVQALVRWIAPILSFTADEIWQ"
BASE COUNT          757 a          806 c          823 g          762 t
ORIGIN      
        1 gtagtgatat tcgagccgca accgcaggaa tattttaatg ccgaggcggc gccagcccga
       61 ttatttggcc tgcgcgataa ggttctagcg ctcaaagccc tcggtgttga ccaagtcctg
      121 tgcctgcgct ttaaccgctg gctgcgcaac ttaaccgccg aggaattcgt ccgccaggtg
      181 ttggtggatt ggctggatgt gcgccatctg gaagtgggtg atgattttcg ctttgggcgg
      241 gatagggctg gcgattacgc gtttttaacc cgcgctgcga ttacctacgg ctttaccctc
      301 gccaataacc ccaccctggt gcgcctgggt gagcgggtta gcagcacgcg gattcgcaca
      361 ttattaagcc aaggggagct ggccgcggcg gcggatttgt tagggcagcc ctacagcatt
      421 agcggtcggg tggtgcacgg ccgggcgctt gcgcgcacac tcgggttccc caccgcgaat
      481 atcgccatcg gcaattaccg gcgggcgctt gccggtgttt atgcggtgga ggtagaaggg
      541 ccctgcgggt gcctgccggc ggtggcgaat ataggtgtta aacccaccat cgccgatggc
      601 cttaaaccca gtttggaagt atttatttta aacggaagct acgaattata cggtgcgcgc
      661 ctgcgcgtgc ggtttttgcg ctggatccgc gccgagcaaa aatttatcaa tattgaagcg
      721 ctcaaacagc aaattcagcg agacgtaaag gccgccgctg atttttttac tacccgtcaa
      781 acctaaaaaa acccattttt taactcaagc cctatcaagc gaatgaccat gaccgactac
      841 aaggccacat taaatctgcc tgccaccacc tttgccatga aagcgaattt ggcccagcgc
      901 gagccggaaa tgctcaagcg ctggcagcag gaaggtattt accagcagat tcgcgccgcc
      961 cgcgcaggct gccccacatt tattttgcac gacggccctc cttacgctaa cggcgatatt
     1021 cacatcggcc acgcggttaa taaaattctt aaagatatta ttatcaaagc caaaaccctc
     1081 agcggttttg atgcgcccta catccccggc tgggattgcc acggcctgcc cattgaacac
     1141 aatgtggaga aaaaaattgg caaagccggt gtaaaagtgg acgtagcgac ctttcgcagg
     1201 cactgccgcg aatacgccag caaacaagtg gccggccaaa aagccgattt tattcgcctg
     1261 ggagtattgg gcgaatggga taaaccctat ttaaccatgg actataaaac cgaagccgat
     1321 accgtgcggg cgctggggaa aatcattgcc aatggccatt tacagcgcgg gtttaaaccc
     1381 gtgtattgga gcgtggtggg cgcctcggcc cttgctgatg cggaagtgga ataccacgaa
     1441 aaaacctcct tttcaattga tgtgcggttt gctgttgagg aagatgcctt gctgcgtaaa
     1501 atcggcgcca taaagggtac aggtccaatt tccgtggtaa tttggaccac cactccctgg
     1561 accttaccgg ccaaccaggc ggtgagttta aatgccgagc tggattacgc tttagtgcaa
     1621 ttaaatgctg agcgcattat cgtggccaag gccttaattg aagccctttg cgcccgcagt
     1681 aaaatcgctg aatttacgat tgtgcaggaa tttaaaggcg ccgatttaga gcatctgttg
     1741 ctgcaacacc cgttttaccc taaacacgtg ccgattattt taggcgacca cgtcaccacc
     1801 gatgccggta ccggcgctgt gcatacagcc cccgatcacg gtatggaaga ctttgtggtc
     1861 ggcaaaaaat acaacatcgg cacgcttaat tacgtggatg aaaacggcaa ttactcggca
     1921 caaacaccta tttttgccgg tgagcacgtt tataaagccg atgaaaaagt tgttgcactg
     1981 ttagaagagc gcggcgcttt atttcatcaa ggtaaaatca cccacagctt tccacattgc
     2041 tggcgcacca aaacgccgtt aatttaccgc gcaacacccc agtggtttat tagcatgacg
     2101 caaaataatt tgctggagca ggttaaaacc agtgtgggcg aggttaagtg gttccccact
     2161 tggggggagt cccgtatgcg cggcacctta gaatccagcc ccgattggtg tatttcccgc
     2221 cagcgcactt ggggggtgcc catcacctta tttattcata aagaaacgca aattccccac
     2281 cccaacacgg tggaattaat tgatcaggta gcccagttaa ttgaacaaaa gggtatggat
     2341 gcctggtttg aattggatgc agctgaatta ttaggcgatg aagcgagcca ctacacaaag
     2401 gtcaccgaca ccttggatgt ttggtttgat tctggcgtta cccattattc ggtgctggag
     2461 cagcgcccgg aagtgagctt cccggcggat ttgtatttag aaggttctga ccagcatcgc
     2521 ggttggtttc aatcctcctt aaaaacctcc gtcgccatga atggctgcgc tccttacaaa
     2581 caagtattaa cccacggttt tgcagtggat gaaaaaggct taaaaatgtc caagtccttg
     2641 ggcaatgtgg tgccaccgca aaaagttgtc aacgatttag gagcggatgt attgcgcctg
     2701 tgggtggcag caacggattt tagcggcgat atgagcgttt ccgacactat tttaaatcgc
     2761 accgccgatt cctaccgccg tattcgcaat acagtgcggt atttattatc caacctgccg
     2821 gattttaatc ccgctacgga tttaattgct cttgatgaca tggtggccct cgaccgctgg
     2881 gccgtggcga gggctgcggc cctgcaaggt gaaatcatca gccattacga cgcctatgat
     2941 ctgcatttaa tttatcaaaa actgcacaat ttctgcgtgg tggatttagg cggtttttat
     3001 ttggatatca ctaaagatcg cgtctatacc tgtgcgcaaa attctgcggc ccgccgcagc
     3061 gcccagtcgg cccaatacca tattgtccag gccttagtgc gctggattgc gccgattttg
     3121 tcctttactg ccgatgaaat ttggcagg
//
LOCUS       DAOQGS010000486         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18193_length_3146_cov_4.846328, whole genome shotgun sequence.
ACCESSION   DAOQGS010000486 DAOQGS010000000
VERSION     DAOQGS010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18193_length_3146_cov_4.846328"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKZ52_15070"
     CDS             complement(<1..421)
                     /locus_tag="PKZ52_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61142.1"
                     /translation="MTEQVQRRHHRHRKVGVWDAFVKLTIGNVFTFMGWCVVAIGISI
                     LIEWGGMMAAWWAPNHSKIMLDDELSFLAAFPRNFITGLYPGDIAVTFISGTQDLLQK
                     LTLPAWADGFKRAGNGFAQLIGYGLESAINTVIIFMCR"
     gene            complement(418..2622)
                     /gene="traD"
                     /locus_tag="PKZ52_15075"
     CDS             complement(418..2622)
                     /gene="traD"
                     /locus_tag="PKZ52_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HNG61143.1"
                     /translation="MHSYPLEQLLRRPVELQAAMGYGLAAGLVALSPSVVMGAVDTPW
                     MTGGILTALLTRGGMRFYEAYRLIKYQYQLHVLPEYKLDSKAIPKSKEKLFLGLGFEW
                     RSRHTQRIVDFSRGEFDYHKDRATRLSYRCARKIETFANKPWLAKWIPPLVKPLGLDK
                     KPFFEKIGSKALLTGWIATLLATQSWANPWAPIPYTEGNPEFHAVGLWEGEKPIFEMQ
                     AERVAHKFVVGTTRVGKTRLAEVFIDQDIANGDVVIVFDPKGDADLLKRCYISAQRAG
                     RGHQFYCFHLGYPEVSARYNPIGNFGRITEPASRVASQLPGEGHSAAFREFTWRYVNV
                     MSKALVTIGKTISYKTLLHYGADIDPLLSEYIEFLFKQDINQRALRRIGAGDWGKMVD
                     ILAESIKPDKTQAMRDKRALALVDIYKRAGLTDPTAGALIKTFEYEKSFYDKLVASLF
                     PLLEKLTTGPAGELLSPDYLDELDKRPIFDWDTVIRTGGIVYVGLDALSDAEVAQAVG
                     NSMFSDLTSLAGSIYKHGIHQGLPEKYATSIFKRKISIHADEFNELVGKEFIPMANKA
                     GGSGYQLTVYTQTMSDIEARFGSAAKAGQVIGNLGNLVMLRVKELKTAKLLTDLLPEA
                     EINQLTTVSSAQDDVNPETTTDFTSGTQQRITTRNVPLLSPGDLMRLPKGHAFAMLGG
                     TLYKIRIPQFTPEADLPENLSFLYEDMKKRYATASANENWYRLPEIPWEDAA"
BASE COUNT          796 a          762 c          759 g          829 t
ORIGIN      
        1 gccgacacat aaaaataatc actgtattga ttgcactttc taatccgtat ccgatcagct
       61 gtgcaaagcc attgccggca cgtttaaaac cgtccgccca ggcgggcaat gtcagttttt
      121 gtaataaatc ctgagtgcca gagataaatg taacggcgat atcgcctgga taaagaccgg
      181 taataaaatt tcgagggaat gcggcaagaa aactcaattc atcatcgagc atgatcttcg
      241 agtgattggg tgcccaccat gcggccatca tgccgcccca ttcaattaat atggaaatac
      301 caatggcaac aacgcaccag cccataaatg taaatacgtt gcctatcgtc aattttacga
      361 aagcatccca aacgccaacc tttcggtgcc gatggtgtcg tcgttgaacc tgctcggtca
      421 cgcggcgtcc tcccagggta tttcggggag gcgataccag ttttcattcg cagatgctgt
      481 cgcatagcgc tttttcatat cctcgtagag gaacgatagg ttttccggca gatcagcttc
      541 tggggtaaat tggggtattc tgattttgta gagtgtgcca cccagcattg caaaagcatg
      601 gcccttaggt agccgcatca aatcgccagg agatagcagc ggtacgtttc gcgtcgtgat
      661 acgttgctgt gtgcccgagg taaaatcggt tgtggtttcg gggtttacat cgtcctgagc
      721 actgctcact gtggtcagct gattaatttc cgcttcgggc aataaatccg tcagtagttt
      781 cgcggttttt agttctttca cccttagcat gacaagattg ccaaggttac ctatgacctg
      841 tcccgcttta gcggcgctac caaagcgcgc ttcaatatca ctcatggtct gcgtatagac
      901 ggtcaactgg taaccactac caccggcctt gttggccatg ggaatgaatt ctttgccaac
      961 gagttcgtta aattcatcgg cgtgaatgct gatttttcgt ttaaaaatcg acgtcgcgta
     1021 tttttctggc aatccctggt gtatgccatg tttatagatt gacccggcta aacttgttaa
     1081 gtcggaaaac atactgtttc caacagcctg ggcaacttca gcatcggata atgcgtctag
     1141 gccgacgtag acaattccac ctgtgcgaat tacggtgtcc cagtcaaata tagggcgctt
     1201 gtctaattca tcgagataat cgggtgatag caattcgcct gcagggccag tcgttaattt
     1261 ttctaacagt gggaaaagcg atgcgaccaa tttgtcgtaa aagctttttt catattcaaa
     1321 cgtcttaatc aatgcaccgg ctgttgggtc ggttaatcca gcgcgcttgt aaatatcgac
     1381 taatgccaat gcgcgtttat cgcgcatggc ttgggtttta tccggtttga tcgattcagc
     1441 cagaatatcg accatttttc cccaatcgcc cgcgccgatg cgccgtaggg ctcgttgatt
     1501 aatatcctgc ttaaatagaa attcaatata ttccgatagg agagggtcaa tatcagcccc
     1561 gtagtgcagg agggttttat agctaatcgt cttgccgatg gttactagcg ctttagacat
     1621 cacgttgaca taacgccacg taaattctct aaacgcggcg ctatgacctt cgcctggcaa
     1681 ttggcttgcg acccgcgatg caggttcagt aatgcggcca aaattaccga tggggttata
     1741 gcgggctgaa acctcgggat accctaagtg gaagcaatag aattggtggc cacggcctgc
     1801 tcgctgtgcg ctgatgtaac agcgtttaag caagtcggca tcccctttgg ggtcgaagac
     1861 gatgacgaca tcgccattag caatatcctg gtcaatgaat acctcggcca gtcgtgtttt
     1921 accgacgcgc gtcgttccca caacaaattt gtgtgcaacc cgttcggctt gcatctcaaa
     1981 gatcggtttc tcaccctccc aaagtcccac ggcatgaaac tctgggtttc cttcggtgta
     2041 gggaattggt gcccatggat ttgcccagga ctgggtagcc agtagagtgg ctatccaccc
     2101 cgtcagcaat gcttttgagc ctatcttttc aaaaaacggc tttttgtcta atcccagtgg
     2161 ttttacgagc ggggggatcc acttcgcaag ccatggctta ttagcaaagg tttcgatctt
     2221 tctagcacat ctataactta aacgcgtcgc tcgatccttg tggtagtcaa attcaccacg
     2281 gctaaaatcg acgattcgtt gggtgtgacg tgagcgccac tcaaaaccca gtcccaggaa
     2341 taatttttct ttagatttag ggatggcttt tgaatctaat ttgtattcgg gtaatacgtg
     2401 tagttggtac tggtatttaa tcagcctata cgcctcataa aaccgcatgc cccctcgcgt
     2461 taaaagtgct gtgagaattc cccccgtcat ccaaggcgta tctacggccc ccattactac
     2521 cgagggtgac agtgcgacta agcctgctgc aaggccatag cccatcgccg cctgcagttc
     2581 tacaggtcta cgaagcaact gttccagcgg atagctatgc acgcgctcac ctaatcaaga
     2641 ttggactagg caagatggta ggcaggtcgt tgtattgaat cagccgttaa tttgactttg
     2701 ctctgtacaa attaatggct gcaagttttg taaaaagccg caagactaaa aggctgaact
     2761 gccgctagaa gggggcttat ccttcggaaa attattttct ctccccctaa taccggggag
     2821 agaaaataat ctgaaagagc taatggataa gggtttttaa gggggaaggg cctggaaggg
     2881 cgaacggtcg ttaaacggca agggaaaagg caaaacccaa aaggccttac ggcaaaaggc
     2941 atgatcggtt gtagactacg gagcgcggcg aaaccgctta acataaacaa ttaatccgca
     3001 aaaaggccat aaaacatgca gacagcatcc atactaaccc cggcacttga ggcgctgggt
     3061 aagcctgaat gggctgattt tttcggcagc ttcctgaata acttttccca tatgcccccc
     3121 atgcaagatg ctgatgctgg cgaatc
//
LOCUS       DAOQGS010000487         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18199_length_3145_cov_4.556311, whole genome shotgun sequence.
ACCESSION   DAOQGS010000487 DAOQGS010000000
VERSION     DAOQGS010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18199_length_3145_cov_4.556311"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="rhlB"
                     /locus_tag="PKZ52_15080"
     CDS             <1..1220
                     /gene="rhlB"
                     /locus_tag="PKZ52_15080"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HNG61144.1"
                     /translation="GFQYCSPIQAQSLPHTLAGHDVLGKAQTGTGKTAAFMIGVIEDL
                     LARPAPEPRYAGEARALIIAPTRELVVQISEDARALCKYSGLSVHALVGGMDYRKQQE
                     KLHREWVDILVATPGRLLDFCGNQDVHLDQIEVLVIDEADRMLDMGFIPQVRRIVRMA
                     PRSSHRQTLLFSATFSPEILDMAKQWMHEPVKVEIEAESVATDRVTQHVYLVSTEEKY
                     TLLYNILKQEGIETLIVFANRRDECRRLHERLREHGFRAGILSGDVDQGKRIKTLDAF
                     KAGQLQVLVATDVAGRGIHIDDVSHVVNFTLPEEPEDYVHRIGRTGRAGNKGISISFA
                     CEDDAFRLPAIETLLGRPIVCEQPPLELLAQEPPSTVGVEKRRPHGGKSAGRPGHGRS
                     GAGRPSPGRARRR"
     gene            1272..1418
                     /locus_tag="PKZ52_15085"
     CDS             1272..1418
                     /locus_tag="PKZ52_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61145.1"
                     /translation="MKTILVVEDSPMVMKIIKHVMGQNHHYQAVFAESFAQAQDCIAQ
                     IGGE"
     gene            1506..2534
                     /locus_tag="PKZ52_15090"
     CDS             1506..2534
                     /locus_tag="PKZ52_15090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG61146.1"
                     /translation="MLTGSFDAARREKLLAKGVVDYVTKEGRFSYVYVSNLLVRLIKN
                     AAVKVLVVDDSNTSRKFITQLLHLQHYQVLEAVDGVDAIKAVIEHPDIRLLITDYNMP
                     RMDGFELVQNLRVKYEKTDLIIIGLSSENDGALSAKFIKNGANDFLRKPFNHEEFFCR
                     VNHNIELLEMIETIRDSAYRDFYTGAYNRHYFFEQGQALLNQAREKKVSIAAVVIDLD
                     NFRDINARYGNDAGDQIMRIIAQKLMHAFGRFLFARSGGQEFYALLYGLDNTKAVALV
                     DLIKERVREPITLGDKQVTVSFSAGVSNGAGFSLDELMTSANNCVLRSKEAGGGLVLG
                     DDDEGEQN"
BASE COUNT          799 a          916 c          778 g          652 t
ORIGIN      
        1 taggctttca atactgctca cccattcagg cccagtccct gccccacaca ttggccggcc
       61 acgacgtatt gggcaaggcg cagaccggca ccggcaaaac cgcggccttt atgatcggcg
      121 taattgaaga cctgctcgcc cgcccggcgc ctgagccgcg ctacgccggt gaagcccgcg
      181 ccctcatcat cgcccctact cgggagctgg tggtgcaaat ttctgaggat gcccgcgccc
      241 tctgtaagta ttcaggcctt agtgttcacg ccctggtggg cggtatggac taccgcaaac
      301 agcaggaaaa actgcaccgc gagtgggtgg atattctggt ggccaccccc ggccgcctgc
      361 tggatttctg cggcaaccaa gatgtgcacc tggaccaaat tgaagtgtta gtgatcgacg
      421 aagccgaccg catgctggac atggggttta tcccacaagt gcgccgcatc gtgcgtatgg
      481 cgccgcgcag cagccacagg caaaccctgt tattttccgc caccttctcg ccggaaattc
      541 tcgacatggc caagcagtgg atgcacgagc cggtgaaggt ggaaatcgaa gccgagtccg
      601 tcgccaccga ccgcgtcacc cagcatgtgt atctggtttc caccgaagaa aaatacacct
      661 tgctgtacaa cattttgaag caagaaggca tagaaaccct gatcgtcttc gccaatcgcc
      721 gggacgagtg ccgccgcctg cacgagcgcc tgcgggagca cggctttcgc gccggtatcc
      781 tctccggcga tgtggaccaa ggcaagcgca ttaaaaccct cgacgccttt aaagcgggcc
      841 agctgcaagt gctggtggcc accgatgtgg ccgggcgcgg cattcacatt gacgacgtct
      901 cccacgtcgt gaatttcacc ctgccggaag agccagagga ttatgtccac cgcataggcc
      961 gcacaggccg cgcgggcaac aaaggtattt ccatcagctt cgcctgcgaa gacgacgcct
     1021 tccgtctgcc cgccatcgaa acgctcctgg gccgccccat cgtctgcgaa cagccgccgc
     1081 tggaactact cgctcaggag ccgccctcca ccgtgggagt ggaaaaacgc cgcccccacg
     1141 gcggcaaaag cgcggggcgg ccaggccacg gccgttcagg cgcaggccgc cccagcccag
     1201 ggcgcgcacg ccgccgctaa ggcagcattc ctgatctaca cttgccaaag gaaccccttt
     1261 ggcaagcagg catgaaaaca attctcgtgg ttgaagacag ccccatggtg atgaaaatca
     1321 tcaaacacgt catggggcaa aaccatcatt accaggctgt ttttgccgag agcttcgccc
     1381 aggcccaaga ctgcatcgcc cagatcggcg gcgaataaat cgctgccgta gtagatttaa
     1441 accttcccga cgccccggat ggggaaattg tcgatttctg cctgagccgc caattaccca
     1501 ccgtagtgct taccggcagc tttgatgccg cccgccggga gaaattattg gctaagggcg
     1561 tggtggatta cgttactaag gaagggcgct tctcttacgt gtacgtgagc aatttattgg
     1621 tgcggctaat taaaaatgcc gccgttaaag tactggtggt ggatgactcc aacaccagcc
     1681 gcaaatttat tacccagctc ctacacctgc agcactacca agtattagaa gccgtagacg
     1741 gtgtagacgc gataaaagcc gttattgagc acccggatat tcgcctactg attaccgatt
     1801 acaacatgcc gcgcatggac gggtttgaac tggtgcaaaa cctgcgggtg aaatacgaaa
     1861 aaaccgattt aattattatc ggtttatcct ccgaaaacga cggcgccctc tcagctaaat
     1921 ttattaaaaa cggcgccaac gattttttgc gcaaaccctt taatcacgag gaatttttct
     1981 gccgggtcaa ccacaatatt gaactgttag aaatgatcga aaccatccgc gacagcgcct
     2041 accgggattt ttacaccggc gcctacaacc ggcattattt ttttgaacag ggccaagcgc
     2101 tactcaacca agcacgagag aaaaaagttt ccatcgctgc cgtggtcatc gacctggata
     2161 atttccgcga tataaatgcc cgctacggca acgacgccgg cgatcagatt atgcgcatca
     2221 tcgcgcaaaa actcatgcac gccttcggcc gctttttatt cgcccgctcc ggcggccaag
     2281 aattttacgc cctgctctac ggcctggata acaccaaagc cgtcgcccta gtggatttaa
     2341 tcaaagagcg cgtgcgcgaa ccgattactt taggcgataa acaggtgacg gtcagtttta
     2401 gcgctggcgt ctccaatggc gcaggcttta gcctggatga gttaatgacc agcgccaata
     2461 attgtgtgct gcgctcgaaa gaagccgggg gtgggttggt gttgggggat gatgacgagg
     2521 gggagcaaaa ttagattttt gcttgttaat ctcagggcat gagtgagcag taaaaataat
     2581 ttgtttcaaa cacaccacaa taaaacttta aaagctaacc catttggacg ctgcatcaga
     2641 gaaaaatatt ttcagtttgc aaccagtcgc tcacctatcg ctcaagcaaa ttcttttata
     2701 ccaaaggtag caccagataa aagacaacac acaacccgct tttatcacta acaaccacca
     2761 agggcgccac catgcgccag tgcctgcttc tataaaatca ctaatgctaa acacaaaaaa
     2821 tgtcagtgcc gccacaccta aaacccgagc ataccgtccg tgcaagccat caatcacgag
     2881 cctactcagc aacccaaagc ccatcacaca ccatagccct gattcaatat aattaaattg
     2941 cgcaatatcc aatatcttca ccaatcactc catataccaa aataagcatt aaaaagcagt
     3001 tacttaacac cctggatgtt tttgccgctc cccttttaaa gcgcagggat ggggggccat
     3061 gtcattaagt taagggaaag gcccgttctg gcgtaggtca atgagatgga ctccccctct
     3121 ctaacaaccc ggcatcgaag tgcca
//
LOCUS       DAOQGS010000488         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18202_length_3145_cov_3.578964, whole genome shotgun sequence.
ACCESSION   DAOQGS010000488 DAOQGS010000000
VERSION     DAOQGS010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18202_length_3145_cov_3.578964"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKZ52_15095"
     CDS             complement(<1..304)
                     /locus_tag="PKZ52_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNG61147.1"
                     /translation="MNPALDLLQPYPFEKLAQLKAGLTPVAKPHIALSIGEPKHAPPT
                     FVVKHLIEQLGGLSNYPTTKGLLELRSAMAQWATARFNLQAQPLDPERHILPVAGTR"
     gene            complement(338..3031)
                     /locus_tag="PKZ52_15100"
     CDS             complement(338..3031)
                     /locus_tag="PKZ52_15100"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNG61148.1"
                     /translation="MNQSLCAEFACQPIFFNQTQFRNDLLSKTAIRVFKDAIAGVNCH
                     FDTRFLEGEDVRHLVAERATFIDLVLHYAWHQFDWGLDISLIAVGGYGRGELHPHSDI
                     DLLILLADSAEDKYSEQISQLITFLWDIGLEIGSSVRTLQECVDIAKNDITVATNLME
                     ARRIAGNDRLRDQLQILTGPEYMWPVSEFFHAKYQEQHERHKKHNYTESNLEPNVKNA
                     PGGLRDIQTINWVAKRFFGVQTLRQLEGKDFFTEQEFAALRHGEAFLWKVRYGIHRLN
                     RRPDERLLFEHQRELAKLFGYVDNHKELAVEQFMHDYYRAVQALRELNDVLLQYLDEV
                     IHANNSPSITPINAHFQLHEAHIEVTHPQVFAEHPSALMEIFLIMGQRNDIVGVRAET
                     IRLIRENRFLVNDSFRQTPAINAMFMDLLRIENGLITQLTRMKRYGILGRYLPEFGRI
                     TGQMQFDLFHRYTVDAHTLLVIKNMRRFRLPEAQEEFPIAAHIMRRLPQPELLYIAGI
                     FHDIAKGRGGDHSILGVEDATAFCQQHGISPRHTRLVAWLVEKHLLMSYVSQKKDISD
                     PEVIRQFALQVGDQMHLDYLYTLTVADMCGTNPEIWNAWRASLMRQLYLETKRALRRG
                     LENTIDQQDIIEETQRQAIDKLADKGMSETWVRSIWGDMGKDYFLREGHLDIAWQTEA
                     IAKHNSDAPLILIRSAGARLWHGATEIFIRTKDVNHVFLAVATCFAQMGFNIQDARLY
                     STNSGYTVDTFYVLDEKNQPLDDDPALYEKIRQSLVDELSLVNDYSEVIKRRTPRELK
                     QFATPTRTRISNEDPSGYTVLEVISPDRHGLLATLGRIFMDFKIQIQNAKISTLGERV
                     EDVFFISSQHGQQLKDPEFYQALQKTICQTLDLNVEQDARY"
     gene            complement(3049..>3145)
                     /locus_tag="PKZ52_15105"
     CDS             complement(3049..>3145)
                     /locus_tag="PKZ52_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNG61149.1"
                     /translation="KDGRLSAQWEHTLAVTASGYEVLTARKEERF"
BASE COUNT          721 a          729 c          856 g          839 t
ORIGIN      
        1 cccgtgtgcc ggccacaggc aaaatgtggc gttccgggtc aagcggctgc gcctgtaaat
       61 taaaacgcgc cgtggcccac tgggccatgg cgctgcgcaa ttctaagagg cctttggtgg
      121 tggggtaatt ggataggccg cctaattgtt caattaagtg ttttaccaca aaggtgggcg
      181 gcgcgtgttt gggctcgccg atggagaggg caatatgggg cttggcgacg ggtgtaaggc
      241 ctgcttttaa ttgggccagt ttttcaaacg ggtagggttg taataaatcc aaagccgggt
      301 tcataaaggg cctttttggc gtgatgcatc aatggggtta ataacgggcg tcctgctcga
      361 catttaaatc gagggtctgg cagatggttt tttgcagggc ttggtaaaat tccgggtctt
      421 ttaattgttg gccgtgttgg ctggaaataa aaaacacgtc ttccacgcgc tcacctaggg
      481 tggagatttt ggcgttttga atttgaattt taaagtccat aaaaatgcgc ccgagggtgg
      541 ctaacaggcc gtgtcggtcg gggcttatta cttctaaaac ggtgtagccg ctggggtctt
      601 cattgctgat gcgagtgcgt gttggggtgg caaattgttt taattcacgg ggggtgcgcc
      661 gtttaatcac ctctgaataa tcattcacca ggcttaattc atccaccagg ctttggcgga
      721 ttttttcata gagggctggg tcgtcatcta ggggttggtt tttttcatcc aacacataaa
      781 acgtatccac cgtgtagccg ctgttagtgc tgtagagcct ggcgtcttga atattaaaac
      841 ccatttgggc aaaacaggtg gccacagcta aaaatacgtg gttgacatct ttggtgcgga
      901 taaaaatttc cgtggcgccg tgccagagcc tcgcacctgc gctgcggatt aatattaagg
      961 gggcatcgct gttgtgtttg gcaatggcct cagtttgcca ggcgatatcc aaatgaccct
     1021 cccgcaaaaa ataatccttg cccatatcgc cccaaatgct gcgcacccag gtttcgctca
     1081 tgcctttatc ggcgagttta tcaatcgcct gccgttgggt ttcttcaata atatcctgct
     1141 ggtcgatggt attttctaag ccccgccgca aagcccgttt ggtttctaaa tacaattggc
     1201 gcattaggct tgctcgccag gcattccaaa tttccgggtt ggtgccgcac atatccgcaa
     1261 cggtaagcgt gtagagataa tccaaatgca tttgatcgcc cacttgcagg gcaaattggc
     1321 ggatgacttc cgggtcggaa atatcttttt tctgcgatac ataagacatc aacaaatgtt
     1381 tttccaccaa ccaggccacc aggcgtgtat ggcgggggga aataccgtgt tgctggcaaa
     1441 atgcggtggc gtcttccacc cctaaaatag agtggtcgcc gccgcgcccc ttggcgatat
     1501 cgtggaaaat tccggcgata tacagcagct caggctgggg caggcgtcgc ataatatggg
     1561 ccgcaatggg gaattcctcc tgggcttccg gcaggcgaaa gcggcgcata tttttaatca
     1621 ctaataaggt atgggcatct accgtatagc ggtggaataa atcaaattgc atttggccgg
     1681 tgatgcggcc gaattccggc aggtaacggc ctaaaatgcc gtagcgtttc atgcgggtaa
     1741 gctgggtaat taagccgttt tcgatgcgca ataaatccat aaacatagcg tttatggcgg
     1801 gggtttggcg aaatgaatcg ttaactaaaa agcggttttc acggatcagc cgaatggttt
     1861 cggcgcgcac acccactatg tcgttgcgct ggcccatgat taaaaatatt tccatcaatg
     1921 cgctggggtg ttcggcaaat acctgcgggt gggtcacttc aatgtgggct tcgtgcagct
     1981 gaaaatgcgc gttaatgggc gtaatacttg ggctgttgtt ggcgtggatg acttcgtcca
     2041 ggtattgcaa taacacgtcg tttaattccc gcagagcttg cacggcgcgg taataatcgt
     2101 gcatgaattg ctcgacggcc agctctttgt ggttgtccac ataaccaaat aatttggcta
     2161 gttcccgttg atgttcgaat aataggcgtt catccgggcg gcgatttaaa cggtggatgc
     2221 cgtagcgcac tttccataaa aacgcttcgc cgtggcgcag ggcggcaaat tcttgttcgg
     2281 tgaaaaaatc cttaccctcc aattgccgca gggtttgcac accgaaaaat cgcttggcca
     2341 cccagttaat cgtttggata tctctaaggc cgccgggggc gtttttgaca ttgggttcta
     2401 aattggattc ggtgtaatta tgttttttgt ggcgctcgtg ttgctcttga tacttagcgt
     2461 gaaaaaattc actcaccggc cacatgtact caggcccggt tagaatctgc aattggtcgc
     2521 gcaggcgatc attgcccgca atgcggcgcg cctccattaa attggtggct acggtgatgt
     2581 catttttagc tatgtccacg cactcttgca gggtgcgcac actgctgccg atctcaaggc
     2641 ctatgtccca caaaaaggta attaattggc tgatttgttc gctgtattta tcttcggcgg
     2701 aatcggccag caaaattaat aggtcgatat ccgagtgtgg gtgtaattca ccgcggccat
     2761 aaccgcccac agcaatcagc gaaatatcca ggccccagtc aaattggtgc caagcgtaat
     2821 gcaggactaa atcgataaaa gtggcccgct cggccactag gtgacgcacg tcttcaccct
     2881 ctaaaaagcg tgtgtcgaaa tggcaattaa ccccggcaat ggcgtcttta aaaacgcgaa
     2941 tggcggtttt gcttagaaga tcgttgcgaa attgggtttg attaaaaaaa atcggctggc
     3001 aggcgaattc ggcgcacagg gattgattca tactgggtac ctcaaaagtt aaaatcgctc
     3061 ctccttgcgt gcggttaata cctcatatcc gctggcggtc accgctaggg tgtgttccca
     3121 ctgggcgctt aagcggccgt ctttg
//
LOCUS       DAOQGS010000489         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18218_length_3142_cov_4.798510, whole genome shotgun sequence.
ACCESSION   DAOQGS010000489 DAOQGS010000000
VERSION     DAOQGS010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18218_length_3142_cov_4.798510"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKZ52_15110"
     CDS             <1..528
                     /locus_tag="PKZ52_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG61150.1"
                     /translation="GSLAINVSTWQFARPDFVRNTQQLISQSGVPPERVTLEVTESAL
                     MADVQEVVNKLNQLRDFGVKIALDDFGTGYSSLAYLRDLPLDALKIDKSFVDEVAANP
                     HQPLVESMIAIGKHMQLKVVAEGVETQQQLIHLKAMGCHFYQGYLFSKALPKAEFEKW
                     LQKNEVLRNIDRQLR"
     gene            835..1140
                     /locus_tag="PKZ52_15115"
     CDS             835..1140
                     /locus_tag="PKZ52_15115"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG61151.1"
                     /translation="MARKSDIDVMSEQISALVQGVTATSRAVELVSQQMTNERKRTND
                     LMSATFESLSKRERPVFVKTLRKQGYTQEEVGEAIGRSQSAVHQYEKKYDMHAQKIK"
     gene            1565..1801
                     /gene="rpmB"
                     /locus_tag="PKZ52_15120"
     CDS             1565..1801
                     /gene="rpmB"
                     /locus_tag="PKZ52_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNG61152.1"
                     /translation="MSKVCQVTGKGPITGNNVSHAKNHTRRRFLPNLHTHRFWVESEK
                     RFVKLRVSSKGMRIIDKNGIEQVLADIRARGLSV"
     gene            1827..1982
                     /gene="rpmG"
                     /locus_tag="PKZ52_15125"
     CDS             1827..1982
                     /gene="rpmG"
                     /locus_tag="PKZ52_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNG61153.1"
                     /translation="MRDKIRLNSSAGTGHFYTTTKNKRTMPEKMEIKKYDPVVRQHVI
                     YKEGKIK"
     gene            2059..2901
                     /gene="mutM"
                     /locus_tag="PKZ52_15130"
     CDS             2059..2901
                     /gene="mutM"
                     /locus_tag="PKZ52_15130"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNG61154.1"
                     /translation="MPELPEVETTRRGIAPHCLGKTVAQLIVRQGQLRWPVPAALSRI
                     LPGQTLLAVERRGKYLLLQFAAGTAIWHLGMSGSLRVVEAASAPGKHDHIDLCFTTGL
                     CLRFNDPRRFGALLWQPSNEPIEQHSLLAHLGPEPLSEGFDSVYLFERSRKLRVALKP
                     WIMDAKVVVGVGNIYANESLFNARLHPEQAAGSLTRKQAERWVAAIKTILQYAITRGG
                     TTLRDFVGGDGKPGYFAQELNVYGRGGEPCTACAKSLVEIRLGQRSTVYCPRCQKLPK
                     AKVR"
     gene            2909..>3142
                     /locus_tag="PKZ52_15135"
     CDS             2909..>3142
                     /locus_tag="PKZ52_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNG61155.1"
                     /translation="MHIKTMGTGLGVSIWGLAWWAMLGPASGWDFTNLAMILMLASTL
                     AAVLLPIRLAAATLVVSVLAFDWLLIEPRGSLVP"
BASE COUNT          724 a          773 c          854 g          791 t
ORIGIN      
        1 ggcagtttgg ctattaatgt ctccacctgg cagtttgcac ggccggattt tgtgcgcaat
       61 acccagcagt taatcagcca gtccggtgtg ccgccggagc gggtcacttt ggaggttacc
      121 gaaagtgcct taatggccga tgtgcaggag gtggtgaata aactcaatca attacgggat
      181 tttggggtaa aaattgccct ggatgatttt ggcaccggtt attcgtcgtt ggcgtatttg
      241 cgagatttac ctttagatgc gctgaaaatt gataagagtt ttgttgatga ggtggcggcg
      301 aacccccatc agccgctggt ggaatccatg atcgccatcg gcaaacacat gcaattaaaa
      361 gtagtggctg aaggggtgga aacccagcag cagctcattc atttaaaggc catgggctgc
      421 catttttacc agggctattt attctccaaa gcgctgccca aggccgaatt tgaaaagtgg
      481 ctgcagaaga atgaggtgct gcgcaacatt gatcgccagc tgagataaca ggagtcaatc
      541 aaaaagtacc ggcccattgt cttaaattgt gcagccacgg gaccaaatcc agattgctat
      601 cttcaaaggt gccgcttaaa gttaggcggc aacctcggct gcgcggcgat cctgctaaaa
      661 gttttaattt gcgcaactgt ctttgttatg tgctcacgct caggacttgt taattagcat
      721 cttctaatgt aactcctctt ccctgggtgt attcgattat tattcgaaaa atatttgatt
      781 ttaatttggt cttctttata cttaaatcat ctttaactga gactggaggc cgaaatggct
      841 cgtaaaagtg atattgatgt aatgtcagag caaatatcag ctctggttca aggtgttact
      901 gcaacatcga gagcggttga gctggtctcc cagcagatga ccaatgagcg taagagaacc
      961 aatgacttga tgtctgctac cttcgaatcc ctttccaaaa gggagcgccc cgtatttgta
     1021 aagactctca gaaaacaagg ttacactcag gaagaggtag gtgaagctat cggacgatct
     1081 caaagtgccg tgcatcagta cgaaaaaaag tacgacatgc acgcccagaa gattaagtag
     1141 ctatttctga atagcgctgt acttcgtgag tgcagcgtat aaatgtctgc gcacataaca
     1201 agccaatgca cgcggagcca gctacgctcc acagttattg tggtattgct gcgctccttt
     1261 accataataa ttgttccact acgctggccc ggtgattgga gcgttcaggc gtggtggtgt
     1321 tttgctaggt aggcacagcc aaaaggcgtg cttctggaag gggcgtccca atggggcggg
     1381 ggcggcaata aattcgttgc tatgcccaag ctgttctggt ataaaacgcg cctcttttcg
     1441 gccacccccc ggcaattgat gtttttcgat ccgccacggc cgtgaatttg cgcatgtagc
     1501 taaacttttt cctatcgccg cccaattcca gcgggcagcg gttttcttca tatggggcag
     1561 gacaatgtct aaggtatgtc aggttacggg taaaggcccg ataactggta acaacgtatc
     1621 acacgcaaaa aaccacactc gtcgtcgttt tttgcctaac ttgcatactc accgtttttg
     1681 ggtggaatca gaaaagcgct ttgtaaaact gcgcgtttct tctaagggta tgcgtatcat
     1741 cgacaaaaac ggtatcgagc aagtgttggc tgatatccgt gcccgcggcc ttagcgttta
     1801 agctgcggaa ctgaggagag agcaccatgc gtgacaaaat tcgcttaaat tccagcgccg
     1861 gtaccggtca cttctacacc acgaccaaaa acaagcgcac catgcctgag aaaatggaga
     1921 tcaaaaaata cgatcccgtt gttcgccagc acgtaatcta caaagaaggc aaaattaagt
     1981 aattgatgcg cctttgaata aaacccagcc ccgctgggtt ttttttcgcc tgtaatttgc
     2041 gctcaaagcg gggtttgtat gcctgaattg cctgaagttg aaaccacccg tcggggcata
     2101 gcgccccatt gtttgggtaa aacggttgcc cagctgattg tgcgccaggg ccagttgcgc
     2161 tggccggtgc ccgccgcgct cagccgcatt ctgcccggcc aaaccctgtt ggcggtggag
     2221 cgccggggta agtatttatt gctgcaattc gccgctggca ccgccatttg gcatctgggt
     2281 atgtccggta gcctgcgggt ggtggaggcg gcaagtgcgc ccggcaaaca cgatcatatc
     2341 gacctgtgtt ttaccacagg cctgtgcctg cgctttaacg accctcgccg cttcggcgct
     2401 ctgttgtggc agcccagtaa cgagcccatt gagcagcaca gcctgctggc ccatttgggg
     2461 cctgagcctt tgagcgaggg ttttgatagt gtttatttat tcgagcgctc ccgcaaattg
     2521 cgggtggcgc ttaagccctg gattatggat gccaaggtgg tggtgggggt ggggaatatc
     2581 tacgccaatg agtccctatt taacgcccgt ttgcacccgg agcaagccgc cggcagccta
     2641 acccgaaagc aggcggagcg ctgggttgcg gccattaaaa ccatcctgca gtacgccatt
     2701 acccggggcg gcaccaccct gcgggatttt gtcggtggcg atggcaagcc aggttatttc
     2761 gcccaggaac tcaacgtcta tggccgcggc ggcgagccgt gtactgcctg cgcaaaatcc
     2821 ttggtcgaga ttcgtttagg gcagcgctca accgtctatt gcccccgctg ccaaaaactg
     2881 cctaaagcca aagtacgcta gcccgcttat gcatatcaaa accatgggca caggcttggg
     2941 tgtgagtatt tggggcctgg cttggtgggc catgctgggg ccggcaagcg gctgggactt
     3001 taccaatctc gccatgattt taatgctggc ttctacactg gcagcggtgt tactgcctat
     3061 tcggttggct gcggccactt tggtggtctc ggtgctggcg tttgattggt tgttgattga
     3121 gccgcgcggc agcttagtgc cg
//
LOCUS       DAOQGS010000490         3136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18267_length_3136_cov_4.016228, whole genome shotgun sequence.
ACCESSION   DAOQGS010000490 DAOQGS010000000
VERSION     DAOQGS010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18267_length_3136_cov_4.016228"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..837
                     /locus_tag="PKZ52_15140"
     CDS             223..837
                     /locus_tag="PKZ52_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNG61156.1"
                     /translation="MKHIKPLWLSAVLGTVLASASVLPAQAAEYVIDTKGAHAFIQFR
                     VKHLGYSWLYGRFNDFSGSFTYDDAKPEASKIAVDIKVTSLDSNHAERDKHLRSKDFL
                     DTDAFPTAKFVSTSYKSTGPKTADLSGDLTLHGVTKPVVIAVEAIGAGKDPWGGYRAG
                     FEGKTTIKPADFGIEMEKKLGPASGEVELFLTVEGVRQAEGVKK"
     gene            970..1842
                     /locus_tag="PKZ52_15145"
     CDS             970..1842
                     /locus_tag="PKZ52_15145"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNG61157.1"
                     /translation="MNWFKLYEFAAGQDLSVLAHNLQAAQLPHRFTDELDRQILWVPD
                     KYTAQQAQLLIELYAREPSKRVIAAAPAPTSKLAIPSVRTIPITYTLLALSIIGYLFG
                     LAPPAWRQPLLFFPIPLDGSVWPAVWHYWVATGEWWRLITPAFLHWSAVHIIFNALAL
                     VDFGGRLERQLPRLVYMGLFLFTAIVSNFGQFTFVPQVGFGGISGALFGYFGCIFAIS
                     WLVPASNLRLPKVFVVFSLVMILAGIFGLLDSIIGPMANGAHLTGLAAGFVYGIGVGI
                     FLKKRLAKPLGEII"
     gene            2242..2949
                     /locus_tag="PKZ52_15150"
     CDS             2242..2949
                     /locus_tag="PKZ52_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HNG61158.1"
                     /translation="MGQIHFIGGEKGGVGKSFTARLLAQYFIDRGQSFLGFDLDASHG
                     TFSRFYGEFTRPIKVDDFASLDTLLITAEAEPDAVLIVDLAAQTAGRLGEWIKQSDAF
                     NLFASLGYEVFLWHVMDDGADALRLLDKLVDTYSNQPVHFVVVKNLGRGEDFTGFDTS
                     ACHTKVQGQNGYVISLERLHGPLAQKIDFNNQSFWSAAQVPNALSLVERQRVKVWLAA
                     QQAQLDRLNLLAVVDAN"
BASE COUNT          745 a          724 c          797 g          870 t
ORIGIN      
        1 ggcggcgtta aatttggcga aaaaatcctc atttactgcg tgtaaactcc gattttttgg
       61 caaattcgcc tggccagcgc tgggtcgccg acatttttca gcgggtcctt aacgctatag
      121 cactaaatac ttgccgatag gttaaatacg gctaagctgt agcggattta tcagttgtaa
      181 aacccataaa aactccactc acacccccat cgaggaaaca ccatgaaaca cataaaaccc
      241 ctatggttat cagctgtttt gggcacagta ttagccagtg ccagtgtatt gcctgcccag
      301 gcggcggaat atgtcatcga caccaagggt gcccatgcct ttattcaatt tcgcgtcaag
      361 catttaggct acagctggtt gtacggtcgc tttaatgatt ttagcggttc atttacctat
      421 gatgatgcca agcccgaagc cagcaaaatt gccgtggata ttaaggtgac gagcctagat
      481 agcaaccacg ccgagcgcga taaacacctg cgttccaaag attttctcga taccgatgcc
      541 ttccctacgg ctaaatttgt tagtaccagt tataaatcta ctggacctaa aacggccgat
      601 ttatctggtg atttaaccct gcacggcgtc accaagccgg ttgtgattgc ggtggaggcc
      661 ataggtgcag gtaaagaccc atggggtggt taccgcgctg gttttgaagg taaaaccacc
      721 attaagcccg ccgattttgg cattgagatg gaaaagaaat tggggcctgc ttccggtgaa
      781 gtcgaattat tcttaacagt agagggggtt cgccaggccg aaggcgttaa aaaatagcgc
      841 ccaattttta cacgcgggtc taaacactca ttaaaaaggc ggttgctagg tgtatatgga
      901 atgcttagcc accgtcgcac taatttcggc ccagcatttt ttattttgtg gcctattcga
      961 ggcaattaat tgaactggtt taaactatat gaatttgcgg ccgggcagga tttaagtgtg
     1021 ctcgcccata atttacaggc agctcaattg ccccaccggt ttacggatga attagaccgg
     1081 caaatcctct gggtgccgga taaatatacc gcccagcagg ctcagttatt aattgagttg
     1141 tatgcgcgcg agccctccaa acgcgtaatt gccgctgccc cagcgcccac tagcaaactt
     1201 gccattccta gtgtgcgcac cattcctatc acctacacgc tattagcgct gagtattatt
     1261 ggctatttat ttggcctcgc gcccccggcc tggcgccaac cattactgtt ttttccaatc
     1321 ccattggatg gctcggtgtg gcctgcggtg tggcattatt gggtggccac gggggagtgg
     1381 tggcgcctta ttacgccggc ttttttacac tggagtgccg tgcatataat ttttaacgca
     1441 ttggcattgg tggattttgg tgggcgttta gagcggcagt tgccgcgcct ggtttatatg
     1501 ggattatttt tatttaccgc cattgtgtcc aatttcggcc aatttacctt tgtgccgcag
     1561 gtgggttttg ggggtatttc cggtgctttg tttggctatt ttggttgcat ttttgccatt
     1621 tcctggctgg tgcccgcttc caatttgcgt ttgcctaagg tctttgtggt gtttagcctg
     1681 gtgatgattc tggcgggcat attcggcttg ctcgattcca taatcggccc catggccaac
     1741 ggcgcccatt taacgggttt ggcagctggt tttgtgtatg gtattggcgt tgggattttc
     1801 ttaaaaaaac gccttgcaaa acctttagga gaaataattt agtggcatta ttagccatac
     1861 ctttgagtga cctgaatcta aaaaccgcag tagggtttaa ctgcggtttt taggttgaag
     1921 gtgccaagct gtatcgtgtc atcttgaaaa cagcttgccc atccatccca tttcctcacc
     1981 cccaagctct gtgtaggcaa gtgttaaaaa tgaacatccg cccgttggca atgggccatg
     2041 tactaacaaa ccgtcttgtt ttgcgcacca gagttgcaag ttgtgcgccc tataaaggcg
     2101 caatattttt tgttttactt cgccgctgaa aaacacagca caatatcgtc gcgataactc
     2161 attaaagcca agcaacgcac gcctttccgc cgtagaatgc ccgctctttg ccagtaggcg
     2221 tactaatgag tgagcgtgtt tatggggcag atacatttta tcggcggtga aaagggcgga
     2281 gtgggcaaat ccttcaccgc gcgtttgctg gcgcaatatt ttattgaccg ggggcaaagc
     2341 tttttagggt ttgatttgga cgcgtcccac ggtactttct cgcgttttta cggcgagttt
     2401 acccgcccca ttaaagtgga tgattttgcc agtttagaca ccctattgat aacggctgag
     2461 gccgagccag atgcagtgtt aattgtagat ttggccgcac aaactgcagg ccgcttaggt
     2521 gaatggataa aacaaagcga tgcttttaat ttatttgcaa gcttagggta tgaggtattt
     2581 ttatggcacg tgatggacga tggcgccgat gcgctacgtt tattggataa attggttgat
     2641 acctacagca accagccggt gcattttgta gtggtgaaaa atttaggccg cggcgaagac
     2701 tttaccgggt ttgacacttc cgcctgccat acaaaggtac aaggccaaaa tggttatgta
     2761 attagccttg agcgcctgca cggccctctt gcgcaaaaaa tagattttaa caaccagagt
     2821 ttctggtccg ccgcccaagt gccaaatgcg ctgagcctgg ttgagcgcca gcgggtgaaa
     2881 gtatggcttg cggctcagca ggcgcagttg gatcgcttga atttattagc tgtggtggac
     2941 gctaattaac ccggcggctt ttcatgccgg gctaataagt tggctgcaaa aaaataaaaa
     3001 acccacagcc taaagaagca ccgtgggaag catggtcaga aatgttacgc ggggtgtatg
     3061 cgcaaaaacg gattaagcat gcgccccagc caatgggtta agcgcaccgg ttgttctaca
     3121 gcggtgaggc ataaac
//
LOCUS       DAOQGS010000491         3130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18332_length_3130_cov_3.400976, whole genome shotgun sequence.
ACCESSION   DAOQGS010000491 DAOQGS010000000
VERSION     DAOQGS010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18332_length_3130_cov_3.400976"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1372)
                     /gene="phaZ"
                     /locus_tag="PKZ52_15155"
     CDS             complement(71..1372)
                     /gene="phaZ"
                     /locus_tag="PKZ52_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HNG61159.1"
                     /translation="MLYYAYELNHAALAPLREAAGFGKKLLESPLNPFAQTFGGRTVA
                     AAFDLFESMTRRYGKPQWRLHETTVNGCPVAIKDRAIWQRPFCQLIHFERDKNALLKA
                     RGKVQADPRVLIIAPLSGHYATLLRRTVESFLPDHEVYVSDWADAREVPVINGKFDLN
                     DYIDYVIDMLHVVGPNANIVGICQPGPPVLAAISLMSEAEDPDLPATMTFMGSPIDPR
                     ESPTVPNKIAMERSYDWFKDNAIYTVPFPNRGVMRRVYPGFLQLGGFISMNQERHVNA
                     HKDYFNHLVEGDCDSVQKHREFYDEYLAVLDLSAEFYLQTIRDVFQEFKLPKRELIHR
                     GRLVKPEAIKTVALMTVEGENDDISGIGQTQAAHDLCTNIPNSMKIDYIQPGVGHYGV
                     FSGTRYRTEIQPRMREFYLNYFNRERETQFQLANPHLIHSS"
     gene            complement(1522..2445)
                     /locus_tag="PKZ52_15160"
     CDS             complement(1522..2445)
                     /locus_tag="PKZ52_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNG61160.1"
                     /translation="MANANFIAAVLQQAIEGNDKVANLDKTEQLIRDAAEKGAELIVL
                     QELHATQYFCQEENTDCFDMAESLDGYTYSRCAQLAEELDIVLVISQFERRAAGIYHN
                     TAQVIDGAEGRVGIFRKMHIPDDPGFYEKFYFTPGDADGELAGFKPVQTRLGKLGVLI
                     CWDQWYPEAARLMALAGADVLIYPTAIGWNPDDTEAEQERQRDAWVTIQRAHAVANGT
                     PVLVANRTGFEDSPLADAAGIEFWGTSFICGPQGEFLVLADSESQGAFCAEINLQQSE
                     TVRRIWPYLRDRRVDAYQGLTRRWLGDKSFN"
     gene            complement(2432..>3130)
                     /locus_tag="PKZ52_15165"
     CDS             complement(2432..>3130)
                     /locus_tag="PKZ52_15165"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNG61161.1"
                     /translation="GGKYAAPDDNQVPTRLADWLGQIPKTSGVVCEGGGLETNGEILL
                     VNQDCIVDEARNPGSTRQSMTEILREWLGVQAIEWLQNIQLTGDDTDGHIDTIARFVA
                     PNTVVYSGTNPHHPDAQSLQRLDTQLKAIAQRRNWQLFALPTPHYTSRIDGRVLPATY
                     ANFLICNQHVFVPIYGLPEDDAALKVLTAACPAWEIIPVRCEALLEQHGSLHCATMQI
                     IDLKNPGSFDYGER"
BASE COUNT          745 a          766 c          827 g          792 t
ORIGIN      
        1 tttggcaaat tcgcctagcc agagcttcgc cgccgacatt tttcagaggt tccctgtatc
       61 cgcaggtgat ttaggagctg tgaattaagt gggggttggc cagttgaaac tgagtttccc
      121 gctcacgatt aaaatagttg aggtaaaact cgcgcatgcg tggctgaatt tccgtgcggt
      181 aacgcgtgcc gctgaataca ccataatgcc ccacgcccgg ttgaatatag tctatcttca
      241 tcgagtttgg gatgttggtg cacaggtcgt gggccgcttg ggtttggcca ataccggaaa
      301 tatcatcgtt ttccccttcc accgtcatca gcgctacggt tttgatggct tcgggtttta
      361 ctaggcggcc gcgatgaatg agttcgcgtt taggcagttt aaattcttgg aacacatcgc
      421 gaatggtttg caaataaaat tcggcgctta aatctaatac cgccaaatat tcatcataaa
      481 attcgcgatg tttttgtacg ctgtcacaat cgccttcaac taaatgattg aaataatcct
      541 tatgggcatt tacgtggcgc tcttgattca tggagataaa accgcccaat tgcaaaaacc
      601 ctggataaac gcgccgcatt acaccgcggt tgggaaaggg cactgtgtaa atggcgttgt
      661 ctttaaacca gtcgtaggag cgctccatgg caattttatt gggtacggtg ggggattccc
      721 gggggtcgat gggggaaccc ataaaggtca tggtggcggg taaatctgga tcttcggctt
      781 cggacattag ggagatggct gcaagaaccg gagggccagg ctgacaaata ccgacgatat
      841 tggcgttggg gccgactaca tgcaacatat caatgacata gtctatgtag tcgtttaagt
      901 cgaatttgcc gttaattaca gggacttcgc gagcgtcggc ccaatcggag acataaactt
      961 cgtggtcggg taaaaacgat tctacggtgc ggcgcaataa tgtggcgtag tggccggaaa
     1021 gcggggcgat gatcagtaca cgcggatcgg cttgtacttt gccgcgggct tttagcagtg
     1081 catttttgtc ccgttcaaaa tgaattaact ggcaaaatgg ccgctgccaa atagctctat
     1141 ccttgatggc aaccgggcat ccattgaccg tggtttcatg caggcgccac tggggtttgc
     1201 catagcggcg ggtcatggat tcgaataggt caaatgccgc cgccactgtg cgcccaccaa
     1261 aggtttgcgc aaacgggtta agcggtgatt ccagtaattt tttaccgaag cccgcggctt
     1321 ccctcagtgg ggctagtgct gcgtgattga gttcataggc gtaatacaac ataacaatcc
     1381 ccttaagcca aaccgcacaa atacgccggt gatggcgcac cttaagggga ttctagcaca
     1441 ctgatgctgc ggcgcagcta aaataattca gtctaggcca taatttccac gccgcctgac
     1501 caagtggggg cggctgctgg cttagttaaa tgatttatcc cccaaccatc ggcgggtgag
     1561 cccctggtag gcatccaccc ggcgatcgcg taaatagggc cagattcttc gcacggtttc
     1621 cgattgttgg aggtttattt cagcgcaaaa tgcaccctgg ctttccgaat ccgctaacac
     1681 caaaaattcc ccttgtggcc cacaaataaa actggtgccc caaaactcaa taccagcggc
     1741 atcggccaag ggtgaatctt caaaaccggt gcgattggcc accagaacgg gcgtgccgtt
     1801 ggccaccgca tgggcgcgct gaatggtcac ccaggcatcg cgctggcgct cttgttccgc
     1861 ttcggtgtcg tcagggttcc agccgatggc ggtcgggtaa atcaaaacgt cggcgcctgc
     1921 cagtgccatt aagcgtgctg cttccgggta ccattgatcc cagcaaatta acacgccgag
     1981 cttgcctaag cgggtttgca ctggtttaaa gcccgcaagc tcgccgtcgg cgtcgccagg
     2041 tgtgaaataa aatttttcgt aaaatcctgg gtcgtcgggg atatgcattt tgcggaaaat
     2101 gcccacgcgc ccttctgcgc cgtcaatgac ttgggcggta ttgtgataaa tcccagctgc
     2161 tcgccgctcg aattgtgaaa tcaccaaaac aatatcgagt tcttctgcca attgtgcgca
     2221 gcgtgaataa gtatagccgt cgagggattc ggccatatca aaacagtcgg tgttttcctc
     2281 ttggcagaag tattgagtgg cgtgtaattc ttgcaaaaca attaattcgg cacctttttc
     2341 agcggcatcg cgaattaact gttcggtttt atctaaattg gcaactttgt cattgccctc
     2401 aattgcctgt tgcaacacgg cggcaataaa attagcgttc gccataatca aaactcccag
     2461 gattttttaa atcgataatt tgcatagtgg cgcagtgtag gctgccgtgt tgttccaata
     2521 atgcctcaca gcgcactgga ataatctccc aagcggggca ggctgcagtg agcaccttta
     2581 acgctgcgtc atcctctggc aaaccataaa ttggtacaaa tacatgttga ttgcaaatta
     2641 aaaaattggc gtaggtggca ggtagtacgc ggccatcgat acggctggtg tagtggggtg
     2701 tggggagcgc aaatagctgc cagttgcgcc gctgggcaat ggctttaagc tgtgtatcca
     2761 aacgctgcaa ggattgtgca tcggggtgat gggggtttgt gcctgaataa acaactgtat
     2821 tgggcgcaac aaaacgggca atagtgtcta tgtgcccatc ggtatcatcg ccggttaatt
     2881 gaatattctg cagccattca atggcctgca cgcccaacca ttcccgcaat atttctgtca
     2941 tggattgacg cgtcgatccg gggttgcggg cctcatctac aatacaatct tggttgacta
     3001 ataaaatctc gccgttggtt tcaagcccgc caccttcaca aacgactccg ctggtcttgg
     3061 gtatttgacc caaccagtcg gcaaggcggg ttggaacctg attatcatct ggagcagcat
     3121 atttaccccc
//
LOCUS       DAOQGS010000492         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18484_length_3114_cov_4.440667, whole genome shotgun sequence.
ACCESSION   DAOQGS010000492 DAOQGS010000000
VERSION     DAOQGS010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18484_length_3114_cov_4.440667"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKZ52_15170"
     CDS             complement(<1..639)
                     /locus_tag="PKZ52_15170"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNG61162.1"
                     /translation="MAENSKIEWTHHTFNPWEGCTKIGPGCDHCYAETRNNRFGGGNW
                     GSGAPRRKTSESNWRLPIKWSKQAAASGVRYRVFCASLADVFDNEVDPQWRVELFQLI
                     KQTPNLDWLLLTKRIGNARAMLPDDWGRGYPNVWLGATVVNQEEAARDMPKLLSTPAA
                     IRWLSMEPLLAPVDFAGMWVDHENPAAHQNMLEALDWIVLGGESGPGARPMHP"
     gene            965..1040
                     /locus_tag="PKZ52_15175"
     tRNA            965..1040
                     /locus_tag="PKZ52_15175"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:998..1000,aa:His,seq:gtg)
     gene            1114..2259
                     /locus_tag="PKZ52_15180"
     CDS             1114..2259
                     /locus_tag="PKZ52_15180"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNG61163.1"
                     /translation="MPEANSVARIFSEHFRCAPDFLLFAPSLVNLMGEHTDYNQGLQI
                     LSTLSRGVWAGFSPRADNQLQLACNFFPDEPRSWELCALVAQDSEFDWSNILRSLTEQ
                     LYSRFSLPIGLNLYLHCQLPASAGFSLIYSLLYATAAALLQANQQAFEAKALADLCWD
                     VKRRHGRLDPTNAKPWVIASGQAGYAYLHDAQQHQLTPLPINPAWQFFVIDLGEPKQF
                     LDSAIAQRTVQCQTAAHALGIAHLSELSLAQLEREKVNLEEIIYKRARHVVSENARCQ
                     QMIQLLGGTDMAALAQCCAQSQRSLQQDYDVTTERLDRWVSQLKNTFGDSIAVRLNGG
                     GFGGSLVLITNRLDGDALQAQVKAVLPELCLLPVRPAGAQLTRFAKS"
     gene            complement(2334..3011)
                     /locus_tag="PKZ52_15185"
     CDS             complement(2334..3011)
                     /locus_tag="PKZ52_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG61164.1"
                     /translation="MDHHHGTALRIAQFDEGQVHAVRQRHGPLERIQIDEAAALQPEP
                     ISLSQETSALDTTRTLTYHEMKQLLNWSQALEAAAPAKIEPRFIVQTAPLAGKVIPLQ
                     GLDVDTRWKIGSDLTSKICLRDASILVDHAYVARNPSGFRLTATSAAKSFLLNGIPLR
                     DSYLRHGDNLQIGRIKLEYRTDSPQEEPAITPSPVAETSSKQNWGVLITLAVIAAAVV
                     LAVFLKK"
BASE COUNT          634 a          855 c          883 g          742 t
ORIGIN      
        1 ggggtgcata gggcgggcgc caggaccgga ttcaccaccg agaacaatcc agtcgagcgc
       61 ttcaagcatg ttttggtgtg ccgcggggtt ttcgtgatcg acccacattc ccgcgaaatc
      121 aacaggcgcc aataaaggct ccatgcttaa ccagcgaata gcggccggtg tgctcaatag
      181 ttttggcatg tcgcgcgccg cctcttcttg gttaactaca gtggccccca gccaaacatt
      241 cggatagccg cgcccccagt catcggggag catcgcccgc gcattaccta tgcgcttggt
      301 cagcagcagc caatcgaggt ttggggtttg cttgatgagt tgaaataact ccaccctcca
      361 ttgcggatca acttcgttat caaacacatc ggccagcgat gcacaaaata cccggtagcg
      421 tacgccgctg gcggccgcct gcttgctcca tttaattggc aggcgccaat tggattctga
      481 ggtttttcgg cgtggagcgc ctgaccccca gttgccaccg ccgaatcgat tgttgcgagt
      541 ttccgcgtag cagtggtcgc agcctgggcc tatttttgtg catccctccc acggattaaa
      601 tgtgtggtgc gtccattcga ttttgctgtt ctctgccatc gctattcctc gtccactatg
      661 aacttaagag tttttgataa atcggtttgc cagttcaccg cccgcaaatc gacgtcggaa
      721 atgcctggca caaatggtta aaaatcaacc aaaaaaaaca ggcaaaaaag gcaaaaaagt
      781 actttcccca cctgggcctg catgtatata ctgcgcgccc tgcagaggcg ccttaaaagc
      841 cttccgggaa aagtaaaaac gagttaagtt attgaaaaat aacggaacgt gtcacggcct
      901 tctaagccgt gggtcgcagg ttcgactcct gccgggcgcg ccattggcac cggcacatac
      961 tatggtgact gtagctcagt tggtagagcc ctggattgtg attccggtgg tcgtgggttc
     1021 gagccccatc agtcacccca ttctcgatat ttccttttgt aaacttacca gtctagtatt
     1081 taactcgtat caaatttgat ccgcgagttt tgtatgcctg aggctaattc cgtcgcacgt
     1141 attttcagtg aacattttcg gtgtgcaccg gattttttgc tatttgcacc gagcctcgtc
     1201 aacctgatgg gggagcacac cgactacaac caaggcttgc agattctctc cacgctcagc
     1261 cgcggtgtgt gggcaggctt ttcgccgcgg gcggataacc agctgcagct ggcctgcaat
     1321 tttttccccg atgaaccgcg cagctgggag ctgtgtgcac tggtggcgca ggatagtgag
     1381 ttcgattggt ccaacattct gcgcagcctc accgagcagt tgtattcccg ctttagtctg
     1441 cccattggcc taaatttata tctgcactgc caactgccag cctcggcggg tttttcactt
     1501 atttacagcc ttttatatgc cactgccgct gcgctgttgc aggccaatca acaggcattt
     1561 gaggccaagg cgctcgccga tttatgttgg gatgtgaagc gcaggcacgg gcggctcgac
     1621 cctactaacg ccaaaccctg ggtgattgcc agcggccagg cggggtatgc atatctgcac
     1681 gatgctcagc agcaccagtt gacgccattg cccatcaatc ctgcttggca attttttgta
     1741 attgatttag gtgagccaaa acagttttta gatagcgcca ttgcccagcg cacggtgcag
     1801 tgccaaacgg ctgcccacgc attgggtatt gctcatctaa gtgagcttag ccttgcccaa
     1861 ctggagcgtg aaaaagtgaa tcttgaggag atcatttaca agcgcgcccg ccatgtggtg
     1921 agtgaaaacg cccgctgcca gcagatgatt cagctcttag gcggtacgga tatggcggct
     1981 ttggcccagt gttgcgcgca atcgcagcgg tcgctgcaac aggactacga tgtgaccacc
     2041 gagcgcttgg atcgctgggt gagccaactt aaaaacacct tcggcgatag catagcggtg
     2101 cgtttaaacg gcggcggttt cggcggcagc ctggtattga ttaccaatag gcttgatggc
     2161 gacgcgctac aggcgcaagt taaagcggtt ttacccgagc tgtgtctgct cccggtaagg
     2221 cccgctggag cacagctgac ccgcttcgcc aagtcttagt tggcgcaacc tgggcacgct
     2281 tgagtgccca gctcgcccgc cttgcacagc tgagcgggca gctctgcaag cgcttacttt
     2341 ttaaggaata cagctaatac tactgcggcg gcaataaccg ccagggtgat cagcacgccc
     2401 cagttttgtt tgctgctggt ttcggcaacc gggcttggag taattgcggg ttcctcctgt
     2461 gggctatccg tgcggtactc cagtttgatg cggcctattt gcaaattatc gccgtggcgt
     2521 aggtagctgt cccgcagtgg aatgccgttg agtaaaaacg atttggccgc tgaggtggct
     2581 gtgaggcgaa agccgctggg gttgcgggcc acataggcgt gatccaccag aatagacgca
     2641 tcccgcaggc aaattttaga ggtgaggtcg ctgccgattt tccagcgggt gtccacatcg
     2701 agcccctgta gcggaatcac tttgcccgcc aggggggcgg tctgcacgat aaaacgcggc
     2761 tcaattttcg ccggtgccgc cgcttccagt gcctggctcc aattgagcag ttgtttcatc
     2821 tcgtggtagg tgagggtgcg ggtggtatcc agggccgagg tttcttggct gaggctgatg
     2881 ggttctggct gcagggcggc ggcttcatct atttgtatgc gctcaagagg accgtggcgc
     2941 tgccgaacgg cgtgaacctg gccttcgtcg aactgggcaa tccgaagggc cgtcccgtgg
     3001 tgatgatcca cggctatacc gacaacgcgc gcgactggtg gccgatgctg ccgtacatgg
     3061 acccgtcgct gcgcctgatc ctggtggaca tccgcggcca cggcgcctcg ggca
//
LOCUS       DAOQGS010000493         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18534_length_3110_cov_3.176105, whole genome shotgun sequence.
ACCESSION   DAOQGS010000493 DAOQGS010000000
VERSION     DAOQGS010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18534_length_3110_cov_3.176105"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKZ52_15190"
     CDS             complement(<1..489)
                     /locus_tag="PKZ52_15190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG61165.1"
                     /translation="MIKLGLWLNGLAHDDLADYASAQLAIAPSREKPQGCSHIVEFSA
                     DGVQLHDCEHSHYKPLKLDFTLGGAAHRRKFGGGAGQMIAKAVGISGKITPHVLDATA
                     GLGGDAFVVACLGCQVTLLERSPIAYVLLADALKRARQFAAQNDSELMQIIERMQLVP
                     GNS"
     gene            complement(482..871)
                     /locus_tag="PKZ52_15195"
     CDS             complement(482..871)
                     /locus_tag="PKZ52_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNG61166.1"
                     /translation="MNQVSSTAAHVGLPRNFLLNKPGASESYPFYPDVPVFKVVNKIF
                     AIGFSKHDNWFLNLKTKPEDGALLCSLFSAITPGYHMNKRHWISVDLAGDLPAWEIER
                     LINTSYDLVVAGFSKKQRLQLIGASGD"
     gene            complement(868..1842)
                     /locus_tag="PKZ52_15200"
     CDS             complement(868..1842)
                     /locus_tag="PKZ52_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HNG61167.1"
                     /translation="MFGAIKKIWAHTAYGQLASQGVLGMNRRNHSYIARYNPRHLYPL
                     VDNKLKTKRLAEEYSVRVPKLVGAIENQHDIILLDSLVASLQGFCIKPCKGSGGKGII
                     VITGRDERGFIKANGQVITAENVQRHISNILAGLFSLGGKPDIALIETLIHADERMTA
                     FTYEGVPDLRVIVFCGFPVMAMMRLSCKASGGKANLHQGAVGVGIDIKTGAAVNAIQN
                     GQSIKVHPDTQQELSALQVPAWQELLELACGCYDMTGLGYLGVDLVLDRDLGPLLLEL
                     NARPGLSIQIANKAGLLPRLQAVEQLKKIERYSNLERVAFSREHFAAQ"
     gene            complement(1845..>3110)
                     /locus_tag="PKZ52_15205"
     CDS             complement(1845..>3110)
                     /locus_tag="PKZ52_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HNG61168.1"
                     /translation="FGLAFVDEGGARRAEWSIRQASGQQTLYYQVDMVAKPESLGGNQ
                     ALPPALRLVSHTGPQAAAAKELLDRARSRSADAYTLARELINEFNTQTQLAQLLNHSQ
                     KRIVWLVDLLNQAGVPAREVLVLNLEDGRRRQSLTSVLQVFENDRYQLFNIDTAEQGQ
                     HPNQLIWQAHAGALLDLAGGSRSKVHFSIIKQEVPVSRVLREKNLSERNLLDFSVHSL
                     PLEDQALFKGILLIPMGVMIVVFLRILVGIRTSGTFMPVLIAMAFIQTNLVPGLIGFS
                     LIVGVGLVIRSYLSRHNLLLVARISAVIITVIILIAIFAILAYRLGLTEGLKITFFPM
                     IILSWTIERISILWEEEGPSEVLTQVGGSLFVAVLAYAVMSNNLVRHLTFNFLGLQFV
                     FMAAVLLCGNYTGYRLTELRRFKPFIGRA"
BASE COUNT          803 a          799 c          771 g          737 t
ORIGIN      
        1 actattgccg ggcactagct gcatgcgctc gattatctgc attaattcag agtcgttttg
       61 tgcagcaaat tggcgggcgc gttttagagc gtccgctaat aatacatagg caatgggcga
      121 gcgctccaac agcgtaacct ggcagcctaa acaggccact acaaatgcgt cgccacctaa
      181 acctgccgtg gcatccaaaa catggggagt tattttgccg ctgatgccga cggctttggc
      241 aatcatttgc ccagcaccgc cgccaaattt gcgccggtgg gcagcgccac ctagggtgaa
      301 gtctaattta aggggtttgt agtggctgtg ttcgcagtcg tgtagttgta cgccgtcagc
      361 gctaaattca actatatggc tgcagccctg gggtttttcc ctgctggggg caatggccag
      421 ttgtgcactt gcgtaatcgg ctaaatcatc gtgcgccaat ccatttagcc acaggcctag
      481 tttaatcacc actggctcca attaattgca ggcgctgttt tttagaaaac cccgccacca
      541 ctaaatcata ggaggtatta ataaggcgct cgatttccca ggctggtaaa tcgccagcca
      601 aatcaacgct aatccagtgg cgtttattca tgtgataacc cggtgtgatc gcgctaaata
      661 ggctgcacaa cagggcgccg tcctcaggct tggtttttag gttcaaaaac cagttgtcgt
      721 gtttgctaaa gcctatggcg aatattttat tcactacttt aaatacaggc acatcaggat
      781 aaaacggata ggattcgctc gcgccaggtt tattgagcaa aaaattgcgc ggcaggccaa
      841 cgtgcgcagc ggttgaagaa acctggttca ttgcgcggca aaatgctcgc gggaaaaggc
      901 tacccgctcc aaattgctgt agcgctcaat ttttttcagt tgctccaccg cctgtaggcg
      961 gggcaataaa ccggctttat tggcaatttg aatggataac ccagggcgcg cattcagctc
     1021 taataacagc gggcctaaat ctcggtctaa cactaaatcc acacccagat aacccaggcc
     1081 cgtcatatcg taacaaccgc aggcgagctc taataattcc tgccaggcgg gcacctggag
     1141 ggcggataat tcctgctggg tatccgggtg cactttaatg ctctggccat tttgaatggc
     1201 attgaccgca gcgccagttt tgatatctat gcccacaccc accgcgccct ggtgtaaatt
     1261 ggctttaccg ccggaggctt tacaggacag gcgcatcata gccataaccg gaaaaccgca
     1321 aaacacaatt acccgcaaat ccggcacacc ctcataggta aatgccgtca tgcgctcgtc
     1381 ggcgtgaata agcgtttcga ttaaggctat atctggcttg ccacctaaag aaaataatcc
     1441 ggccaaaata ttagaaatat gccgctgtac gttttccgcc gtgataacct ggccattggc
     1501 tttgataaaa ccgcgctcat cccggccggt aatgacaata atccctttgc cgccggagcc
     1561 tttgcagggt tttatacaaa agccttgcaa tgatgcaacc agggaatcca gcagaataat
     1621 atcgtgttgg ttttcaatgg cgcctactaa tttaggcacc cgcactgaat attcttcagc
     1681 caggcgtttg gtttttaatt tattatccac taaggggtat aaatggcgcg ggttgtaacg
     1741 ggcaatatag gagtggttgc gccggttcat gcccaacacc ccctggcttg ccaattggcc
     1801 gtaggcggtg tgtgcccaga tttttttaat ggcgccaaac ataattatgc gcgaccgata
     1861 aagggtttaa agcggcgcaa ttcggtgagg cgataacctg tgtaattccc gcataacaac
     1921 acagcggcca tgaatacaaa ctgcaggcct aaaaaattaa aggttaaatg ccgcaccaga
     1981 ttattgctca tcaccgcata ggccagcacc gccacaaata aactgccgcc cacttgggtg
     2041 agcacctcgc tggggccctc ttcctcccat agaatagaaa tccgctcgat ggtccagctg
     2101 aggataatca tggggaaaaa ggtaattttt aagccttcgg ttaaccctaa gcggtaggcc
     2161 aaaatagcga atatagcgat gagaattatc accgtgataa tcaccgcaga aatcctcgcc
     2221 accagcaata aattgtgccg ggataaatag gagcgaatca ccagccccac acccacaatt
     2281 aaactaaaac caattaagcc cggtaccagg ttggtttgaa taaatgccat ggcgatgagc
     2341 actggcataa aagtgcctga ggtgcgtatg cccaccaaaa tgcgcaggaa tacaacaatc
     2401 attaccccca tgggaatcag cagaatacct ttaaataagg cttgatcttc caggggtaag
     2461 gagtgcacgg agaagtctaa taaattgcgc tcgcttaagt ttttctcccg caatacacga
     2521 ctgactggca cttcctgttt aataatagaa aaatgcacct tggagcggga gccgccagct
     2581 aagtctaaca aagcacccgc gtgggcttgc caaattaact ggttcgggtg ctggccctgc
     2641 tcggcggtgt cgatattaaa taattggtag cggtcgttct caaatacttg caatacactg
     2701 gttaaactct gccgcctgcg gccatcctct aaatttaata ccaatacttc gcgggcgggc
     2761 acaccggctt ggtttaataa atccaccagc cacacaatgc gtttttgcga gtggtttaat
     2821 aactgcgcca actgggtttg ggtattaaat tcgttgatca attcccgcgc gagggtatag
     2881 gcatcggcgg agcgggagcg ggctcggtct aataattctt tggccgccgc cgcttggggg
     2941 ccggtgtggg acaccaggcg cagggccggt ggcagggctt ggttgccgcc taaggattcg
     3001 ggtttagcca ccatatccac ttggtaatac agggtttgtt ggccggaggc ctggcgaata
     3061 gaccactcgg cacggcgggc gccgccctca tccacaaacg ccaagccaaa
//
LOCUS       DAOQGS010000494         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18560_length_3107_cov_5.211664, whole genome shotgun sequence.
ACCESSION   DAOQGS010000494 DAOQGS010000000
VERSION     DAOQGS010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18560_length_3107_cov_5.211664"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(408..1514)
                     /locus_tag="PKZ52_15210"
     CDS             complement(408..1514)
                     /locus_tag="PKZ52_15210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG61169.1"
                     /translation="MKLLLVDDHELFRQGLEMLLSSQPTRKTILHAATAAQALQQHAD
                     HPDIDLVILDYNLGNDRGMDVLLSLKQRDPSLPIAVISGREDSEVILAALGAGASGFI
                     PKSLSTDDTLRAINLLLDGAIYAPWPEDPEDQPKAQSERDIARQQQLQHLASFARGVF
                     DTNALHLKEQAAVESQMGSALHSWVSEIQKDRSRLEALAFQDDLTGLANRRLFLERLD
                     QALRNCRRNKTFMALVFLDLDRFKQLNDSLGHYAGDLLLKEIGQRLMRSVREVDTAAR
                     LGGDEFTLILVDVQSEVGLNNQLTRLRASLKEPLEIQNQLIHPSASIGAAISDGSESA
                     TELMARADESLYAVKAQGRDNFVIAAGVNAQGGK"
     gene            2033..>3107
                     /locus_tag="PKZ52_15215"
     CDS             2033..>3107
                     /locus_tag="PKZ52_15215"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNG61170.1"
                     /translation="MLRIFWVFGICLLLAPLLAAEPLDPQAANLRVLVREFQVTGLEH
                     PSRGLSLEALLQQARREQGKFGANMTVEELNQLADALTLYVRGFGFPFHTVYLPPQRV
                     EAGRVELRLQEGLLGAVHVINKTRLQDKRFERPFAAHMHKLLYGPAIEERVQALKNQP
                     GFKVFAFYSRGAKPGEAVLNLRVDEAEQQVYGLRIDNYGSAASGKQRAIAQYSRFELT
                     GRHDRFDLGLLQTLGGVSNRYGSLAYQLPSPELNFLWDISATNNQFELGDRYAALGLE
                     GDVTTLGSGLTWITRPQPAARTSWRAGLYDKRNRLNAGQTALAREVSQAITLEWSRTW
                     ASDKGAVANLQLEYSYGQGQVERL"
BASE COUNT          599 a          819 c          983 g          706 t
ORIGIN      
        1 ggcacttcga tgccgggttg ttagagaggg ggagtccatc tcattgacct acgctatagc
       61 gggccttttc cttaacttaa tgacattgcg gaggggggct ccgaaggggg gtggtagatc
      121 gccgcgctgt tttttttcgc agcccgctgg aagtaaagat ttcgaaataa attcacctca
      181 attaaaagcc gccttaaata aaagctacct caattaaaag ccgccttaaa taaaagctac
      241 ctcaattaaa agccacctga aataaaaacc acctgaaata aaaacagggt tctccactgc
      301 aaccaccccg gcaggcaagc ctgccccctc cgcgggaggg gaattttggg gtgtgcaggc
      361 gccaaaattt cttaacctga atagcatggg gcgagcagtg ctggccttta tttgccgccc
      421 tgcgcattga cccctgcggc tatgacgaaa ttatcccgcc cctgagcttt aacggcgtag
      481 aggctttcat cggcgcgggc catgagttcg gtggcggatt cggagccgtc gctgatggcg
      541 gcgccgatgc tggcggaggg gtggatcagt tggttttgaa tttctaaggg ttcttttaag
      601 cttgcgcgca ggcgggtgag ctggttgttc aggcccacct ccgactgtac gtccaccaaa
      661 atcagggtga attcatcgcc gcctaggcgg gcggcggtgt ctacctcccg caccgagcgc
      721 atcaggcgct ggccgatttc ttttaacagc aggtctccgg cgtagtggcc gagactgtcg
      781 ttgagttgtt taaagcggtc taaatccaaa aataccaggg ccataaaggt tttgttgcgg
      841 cggcagttgc gcagggcctg gtccaggcgc tccaaaaata aacgccggtt ggcaaggccg
      901 gtgaggtcgt cttggaaggc gagggcctcc aggcggctgc ggtctttttg aatttcgctc
      961 acccaactgt gcagggcgct gcccatttgg ctctccacgg cggcttgttc ttttaaatgc
     1021 agggcattgg tgtcgaacac gccgcgggcg aagctggcta ggtgttgcaa ttgttgctgg
     1081 cgggctatat cgcgctcgct ttgggccttg ggttgatcct ctgggtcttc cggccagggg
     1141 gcgtaaatgg cgccgtctag tagcaggttg atggccctta gggtgtcgtc ggtggagagg
     1201 cttttgggaa taaaaccgct ggcgccagcg ccgagggcgg cgaggatcac ttcggaatcc
     1261 tctcggccgg agatcaccgc aatgggcagg gaagggtccc gctgtttaag gctgagtaat
     1321 acgtccatac cgcggtcgtt gcccaggttg taatcgagga ttactaggtc gatgtccggg
     1381 tggtcggcgt gttgctgcag ggcttgggcg gcggtggcgg cgtgcaggat ggttttgcgg
     1441 gtaggctggc tcgacagcag catttccagg ccctgccgga atagctcgtg atcgtccacc
     1501 agtaacagct tcatcgcctt ttaacttcca caaccgtctt tgaatgctta aagaatggat
     1561 gactaaggcc tgcttggcaa ctttgccgcc gggtgagtct tacgcccggg ttaaacggtg
     1621 aggctggtct atagttaaca aaaactagag tgttctactg gtgcgaggcg gttgcccagc
     1681 ggtgtgttgg gtgcccttta aggccctaag cgtgtcgcga atgctgagct tggcggtagt
     1741 taacacggtg gctaattaag aataaatagc tctgggccgc aggttgcagt tgggtgaggt
     1801 atcacctagg caggttttat cgattcccct tcaggcttca cctggtattg cgctgcgcgg
     1861 cagtttctct taagcatttt tcaacaggtt ggtttatgaa ccatggggcg cagccttgtt
     1921 aagacgactt tgggtgttgg gcatctgcct gctgcttgcg cctcatagtt gcgcggcttt
     1981 gtctcaacag cactctttca acaagttggt ttatgaacca tggggcgtag ccttgttaag
     2041 aatattttgg gtgtttggca tctgcctgct gcttgcgccg ctgttggcgg ccgagccgct
     2101 ggacccgcag gcggcaaacc tgcgggtact ggtgcgcgaa ttccaggtga caggccttga
     2161 acaccccagc cggggtttaa gcctagaggc cctattgcag caagccaggc gggagcaggg
     2221 caagtttggc gcgaacatga cggtggagga gctcaaccag ctggcggatg ctttgacctt
     2281 gtacgtgcgc ggttttggct tcccctttca tacggtgtac ctgccgcccc agcgggtgga
     2341 ggcgggccgg gtggagctgc gcctgcagga ggggttgctg ggggcggtgc atgtgatcaa
     2401 caaaacccgc ctgcaggata agcgctttga gcgcccgttt gctgcgcata tgcataagtt
     2461 gttgtacggc cccgccatcg aagagcgggt gcaggcgtta aaaaatcagc cgggttttaa
     2521 ggtgtttgcc ttttattccc gcggggctaa acccggcgag gccgtattga acctgcgggt
     2581 ggatgaggct gagcagcagg tgtacggcct gcgcatcgac aattacggca gcgccgccag
     2641 cggtaaacag cgcgccatcg cccagtactc ccgctttgag ttgaccggtc gccacgaccg
     2701 ctttgatctg ggcctgctgc aaaccctcgg cggtgtctcc aaccgctacg gcagcctggc
     2761 ctaccagttg ccatcgccgg agcttaattt tctctgggac atcagcgcga ccaacaacca
     2821 gtttgagctg ggggatcgct atgcggccct tgggttagag ggcgatgtca ccaccctcgg
     2881 cagcggccta acctggatca cccgcccgca accggcggcg cgcaccagtt ggcgggcagg
     2941 gctgtacgac aaacgcaacc gcttgaatgc agggcaaacc gccctggccc gcgaggtgag
     3001 ccaggccatc accctagagt ggagccgcac ctgggcctcg gataagggcg cggtggccaa
     3061 cctgcagctc gaatacagct atgggcaggg ccaggtggag cggctgc
//
LOCUS       DAOQGS010000495         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18567_length_3106_cov_4.724353, whole genome shotgun sequence.
ACCESSION   DAOQGS010000495 DAOQGS010000000
VERSION     DAOQGS010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18567_length_3106_cov_4.724353"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKZ52_15220"
     CDS             complement(<1..1043)
                     /locus_tag="PKZ52_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61171.1"
                     /translation="MAFALPLLIGFRYTRAKQRNSFISFVSLFAFLGMALGVFALIVV
                     LSVMNGFDAELRERILRAVPHGYITQTGGLADWPKWAEQVRAYPYVKGVAPFIAGQAL
                     LSANGLVRPTELQAIDIKAEPGVSKIHEHIIAGSLEPMAPGEFGMVLGSIAARNMELA
                     IGDKVLVTLPQISVTPAGAFPRAKRFTLVGIFEVGAQVDQSLALINLEDGQKLFKKAD
                     RVDGLRLEFNSIYAAPAYLNQLTAALKTAAGSESEFVARDWSQTQGSLFQAVKMEKTV
                     VSVMLAIIIFVAAFNIVTSLIMMVGEKRSDIAVLRTMGMSAGNVVAIFMVQGIVIGVM
                     GIVIGSLCGIGAAL"
     gene            1280..1630
                     /locus_tag="PKZ52_15225"
     CDS             1280..1630
                     /locus_tag="PKZ52_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61172.1"
                     /translation="MQITLSAAVKTLALMLSLSLPCMAEQIVVPVGSQQGAHVSMPGK
                     GVSMSQVLSRYGEPDHKSGPVGQPPISTWDYPTFTVYFEHQHTIHSVVKHVPAPQTAP
                     AAQAEEPSDDNNEE"
     gene            complement(1756..2142)
                     /locus_tag="PKZ52_15230"
     CDS             complement(1756..2142)
                     /locus_tag="PKZ52_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG61173.1"
                     /translation="MKFGYTIVYVSSVVNTLEFYKRAFGFETRFLHESEQYGELNTGE
                     TVLAFATHAMGEMHFVAGYQKSALGALPLGIELAFVTENVSAAYTKALLAGAVSIAEP
                     CQKPWGQTVAYVRDNDGVIVELCSPI"
     gene            complement(2144..2437)
                     /locus_tag="PKZ52_15235"
     CDS             complement(2144..2437)
                     /locus_tag="PKZ52_15235"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNG61174.1"
                     /translation="MNPKKVTRENLKKLTDLPNIGKAGARDLNLLGIDQPAQLIGRCP
                     YELYYSLCLATGATQDPCVIDVFISVTRFMSGENPLPWWEYTQERKNYLNKGS"
     gene            complement(2454..>3106)
                     /locus_tag="PKZ52_15240"
     CDS             complement(2454..>3106)
                     /locus_tag="PKZ52_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG61175.1"
                     /translation="ILKLVSGKRIKADALLWCNGRTGNTDNLNLSAAGLMANHRGQID
                     VNKHYATAVPSIYAAGDVIGWPSLASASYDQGRAVVSAILGEEVRSVEDAPTGIYTLP
                     EISSIGRTEAELTAAKVPYEVGRAFFKDTARAQISGEDVGMLKILFHIDTLEILGIHC
                     FGAEAAEIIHIGQAIMNQKGSANSISYFTNTTFNYPTMAEAYRVAALNGLNRVQRP"
BASE COUNT          823 a          841 c          729 g          713 t
ORIGIN      
        1 agggccgcgc ctatgccgca caggctgcca atcacaatac ccattacgcc aatcacaatg
       61 ccctgcacca taaatatggc taccacattg cctgcgctca tccccatggt gcgcagcacg
      121 gcaatatcgc tgcgtttttc ccccaccatc atgattaaac tagtaactat attaaaagcc
      181 gccacaaaaa taataatggc cagcatcacg ctcaccacag ttttttccat ttttaccgct
      241 tgaaataaac tgccctgggt ttggctccaa tcccgcgcca caaattcact ctcgctgcct
      301 gcagcggttt ttaaggcggc ggttaattgg tttaaataag cgggcgctgc ataaatggaa
      361 ttaaattcca ggcgcaaacc atcaactcta tcggcttttt taaacagttt ttggccgtct
      421 tctaaattaa tcagggcgag gctttgatcc acctgggcgc ccacttcaaa aatccctacc
      481 agagtaaaac gcttggcgcg gggaaaagcg ccagcgggcg taaccgatat ttgcggcagg
      541 gtcaccaata ctttatcgcc tatggccaat tccatattgc gcgcagcgat actgcccagc
      601 accataccaa attcacccgg tgccataggc tctagggagc ctgcaataat gtgctcatga
      661 attttcgata ccccaggctc ggccttaata tcaatggcct gcaattccgt aggccgcacc
      721 aaaccattgg cgctgagcag ggcctggccg gcaataaagg gtgcaacgcc tttaacatag
      781 ggataggcgc gcacctgctc ggcccatttg ggccaatcgg ccaggccgcc ggtttgggtg
      841 atatagccgt ggggcaccgc ccgtaaaatg cgctcgcgca gttcggcatc aaaaccattc
      901 atgaccgaaa gcaccacaat cagcgcaaat acccccagag ccatgcctaa aaaggcaaac
      961 aggctgacga aggagataaa tgaattgcgt tgttttgcgc gggtataccg aaaaccgatt
     1021 aataaaggga gtgcaaaggc catggaggaa gcgcgtaagg ggctaggaaa cttgcgacgg
     1081 cggcgctacc ctttaaccta caatcactgg gggtacgccg tcgaaacggg ccttaagtta
     1141 ccacttaagg gcgcgcgatt gtaaacggat ggcgctaaag cgcaaataag atccggccct
     1201 gagcgcgctg tttctggttt aatggagccg ttccctgatg ctcccccacc ctgttaactc
     1261 gtcaaaaata gaggcaatta tgcagatcac tctgagcgca gccgttaaaa ccctggcgtt
     1321 gatgttgagc ctaagcctgc cctgtatggc cgaacaaatt gtcgtaccgg tcggctccca
     1381 acagggcgcc catgtcagca tgcccggcaa aggcgtaagt atgagccagg ttttatcccg
     1441 ctatggggag ccggaccaca aaagcggccc ggtgggccaa ccgccgatta gcacctggga
     1501 ttaccctaca tttacggttt attttgagca ccaacacact attcatagcg tggtgaaaca
     1561 tgtgcctgcg ccgcaaactg cgccagctgc acaagccgaa gaacccagcg acgataacaa
     1621 cgaggaataa acccctggcg taaaatcaag cggcaaaaaa agcgggcttg tgcccgcagt
     1681 taatcgcatc gatatgtaac cgctgagttc agcggccaca acttgttcgg tgttggttac
     1741 cttgttcggt gttggttata tcggcgaaca aagttcaacg ataaccccgt cgttgtcacg
     1801 cacataagcc acagtttggc cccaaggctt ttgacaaggc tcggcgatgg aaacagcacc
     1861 tgccaataat gcctttgtgt aagctgccga tacgttttca gtaacaaacg ccaattcaat
     1921 acccaaaggc aatgctccca gggcagattt ttgatagcca gccacaaaat gcatttcacc
     1981 catggcgtgg gttgcaaatg ctaaaacagt ttccccggta ttgagctcgc catattgttc
     2041 tgattcatgc agaaaacgcg tttcaaaacc gaatgcccgc ttgtaaaact ctagcgtatt
     2101 aacaactgac gaaacataaa caattgtgta gccaaacttc atttcagctg cctttattta
     2161 aataattttt acgctcctgc gtatattccc accaaggaag cggattttca ccgctcataa
     2221 agcgtgtgac agaaataaac acatcaataa cacacggatc ctgagttgct cctgtcgcca
     2281 aacagaggga ataatacaac tcataggggc atcggccgat caattgcgcc ggttgatcta
     2341 tgcccagcaa atttaagtcc ctcgcgccag cctttccaat attgggcaag tcggtcaatt
     2401 tttttaaatt ttcacgggtg acttttttgg ggttcatggc agctgataac tggctaaggc
     2461 cgctgcactc gatttaaccc atttaacgcc gccacccgat aggcttccgc catagtcggg
     2521 taattaaaag tggtgttggt aaaatacgaa attgaattag cggagccttt ttgattcatg
     2581 atcgcctggc cgatgtgaat aatctcggcc gcttccgcgc cgaagcagtg aataccgaga
     2641 atttccaggg tgtctatgtg aaagagtatt tttaacatac ccacatcttc accggaaatt
     2701 tgcgcccgag cggtatcttt aaaaaaagcg cggcccactt cgtaaggcac tttagcggcg
     2761 gttaattccg cctcagtgcg gccgatggag ctaatttcag gcagggtgta aataccagtg
     2821 ggggcatctt caacgctcct cacctcctca cccaaaatcg cactcaccac agcccggccc
     2881 tggtcgtaac tggcgctggc aaggctcggc caaccgataa catcacccgc cgcataaatc
     2941 gaaggcaccg ccgtggcgta atgtttattc acatcaattt ggccgcggtg gttggccatc
     3001 aaacctgcag cggataaatt taaattatcg gtattacccg tgcggccatt acaccacagt
     3061 aaagcatcgg cctttatgcg cttgccggag acgagtttta aaataa
//
LOCUS       DAOQGS010000496         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18598_length_3103_cov_4.092848, whole genome shotgun sequence.
ACCESSION   DAOQGS010000496 DAOQGS010000000
VERSION     DAOQGS010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18598_length_3103_cov_4.092848"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKZ52_15245"
     CDS             <1..496
                     /locus_tag="PKZ52_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNG61176.1"
                     /translation="SHGDFLTLWASLPPALRKTTRPVAGADYWLTSPLRRYIANRVFK
                     SVLIPRSLSRTQPNPIELMSEALNAGDSLIIFPEGTRNLGDDLLPFKSGLFHLAQAFP
                     QVEFVPVWIENLGRVMPKGAFIPVPLLCTLIFGEALRLAEGESRQDFLTRSRSALLNL
                     APLN"
     gene            520..1473
                     /locus_tag="PKZ52_15250"
     CDS             520..1473
                     /locus_tag="PKZ52_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNG61177.1"
                     /translation="MTLSESTATLVHLYTGVFVVLILASLIGQGLKMRAHTDGAKSVV
                     ANLNARTNAWWVMVGVLALAMAAGEIGVVALFAFISFQALREFVSLTHTRRGDHRALL
                     WSFFFFLPLQYWLISTHWVGLFMILIPVYGFLLLPISAAIGSDTTRFLERAAKVQWGL
                     MICVYCISHIPALLMLSIPNFDGRQLHLVAFLILVVQSSDVFQYVWGKLMGKRKISPA
                     ISPSKTLEGLIGGILTSTALGASLFWLTPFNLWQAALIALTINVMGFFGGFVLSAIKR
                     DRGVKDWGWLIEGHGGMLDRVDSLSFAAPVFFHIVRYWWVP"
     gene            1848..>3103
                     /locus_tag="PKZ52_15255"
     CDS             1848..>3103
                     /locus_tag="PKZ52_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HNG61178.1"
                     /translation="MRTVIKPLSAMMIVLASAASQAAPVLLGVGEINGSSDLSGLNYT
                     LESGVSAAVLGGTGSGIAYAGGNQFYLLPDRGPNAQSYTNGALVDNTTSYIPRAHKIS
                     IGLSAASSGSLPLSVSAQLESTTLFYSSSALNYGNTAGLASAVPSINTTHKQYFSGRS
                     DNFAPGATTNPNNARLDPEGIRISNNGKTAYISDEYGPYVYEFNIATGERLRTFNLPA
                     SLTAAQSFSTETAEISGNTAGRTTNKGMEGLAITPDGSKLVGIMQATALQDKSKNVRI
                     VVIDIASGTTQEFAYKLTDGTGVSEILAINNNQFLVLERDGKGLGDGSAAVAKKLFQI
                     DLTNAQSVAGLSGDLTSKAVAKTQFLDITSNLIAAGFSANQIPAKLEGIAFGSDVQLG
                     NTQYHTLILANDNDYLNTVGGLANPNR"
BASE COUNT          765 a          815 c          734 g          789 t
ORIGIN      
        1 cagccacggg gattttctca ccctctgggc gagcctgccg cctgccctgc gcaaaaccac
       61 tcggccagta gccggggccg attattggct aaccagcccc ctgcgccgct atatcgccaa
      121 ccgggtattt aaaagcgtgt taatcccccg ctcgcttagc cgaacccaac ccaatcccat
      181 cgagttaatg agcgaagcgt taaatgcggg tgactcgctg attattttcc ctgagggcac
      241 ccgcaattta ggcgatgact tattgccctt taaaagtggc ttatttcatt tagcccaagc
      301 ctttccccag gtggagtttg tgccggtgtg gattgaaaac ctgggccggg taatgccaaa
      361 aggtgcattt attcccgtgc ctttgttgtg caccttaatt tttggcgaag cgctgcggct
      421 agctgaaggt gaatcccgcc aggattttct cacccgcagc cgcagcgccc tattaaatct
      481 cgccccactt aattaactat ttttagccca gagcactcca tgaccctaag cgaatcaacc
      541 gcaacccttg tgcacttata taccggcgta tttgttgttt taatcctggc cagcctcatc
      601 ggccaaggct tgaaaatgcg cgcccataca gatggggcta aaagtgtggt ggccaattta
      661 aatgcccgca ccaacgcctg gtgggtaatg gtaggcgttt tggccctggc catggccgca
      721 ggggaaattg gcgtggttgc cttatttgct tttatttcgt ttcaagccct gcgggaattt
      781 gtctccttaa cccatacccg caggggcgat caccgggcgc tactgtggag ttttttcttt
      841 tttttacccc tacaatattg gctgatcagc actcactggg tgggattatt tatgatttta
      901 attcccgtct atggcttttt attattgcct atctccgccg ccatcggcag cgataccacg
      961 cgctttttag agcgcgccgc caaggtgcag tgggggctga tgatttgtgt ttattgcatt
     1021 tcccacatcc ccgccctttt aatgttatcc atcccgaatt tcgatgggcg gcaattgcac
     1081 ctggtggcat ttctaattct ggtagtgcag tccagcgatg tatttcagta cgtgtggggc
     1141 aaattaatgg gcaaacgcaa aatctccccc gccatttcac cctctaaaac cctggaaggt
     1201 ttaatcggcg gcattttaac ctccacagcc ctcggggcga gtttattctg gttaacacct
     1261 tttaacctct ggcaagcggc gctgatcgcc ttaactatta atgtgatggg ttttttcggc
     1321 ggctttgtat tatcagccat taagcgcgac cggggtgtaa aggattgggg ctggttaatt
     1381 gaaggccacg gcggcatgct cgatcgagtg gactccttaa gttttgccgc ccccgtgttt
     1441 tttcatatag tgcgctattg gtgggtgccc taaataatag ccaaaatgcg cacagattta
     1501 agcgcgcaat atttatgaaa tgcaggctaa aacccagcta acggacaagt cctaagcgcg
     1561 gcagtgcggt tattacacgc gagccctaaa ttggcttagg gaagctattt gttaacctaa
     1621 ctaaggcgcc tcagaaaaat tcacctagcc gccgcaattt ccggaggcag tctatgtaag
     1681 atgccgccct cctcagccaa ttaattgcca cgcaattgtc attaacgccg cataagctcg
     1741 gatcgcgcag gttcaaacct tagatagtga ttaatgctag cagagctcaa agcaacctgc
     1801 tgtctaaacc gccgcctgcg ctttaattaa actaataaga ggtagttatg cgtactgtaa
     1861 taaaaccatt aagtgcaatg atgatagtgc tggcatccgc cgccagccaa gccgcgcctg
     1921 tattattagg tgtgggcgaa atcaatggca gcagcgattt atccggctta aattacacac
     1981 tggaatccgg cgttagcgcg gctgttttag gtggcacagg ttcaggcatc gcttatgcgg
     2041 gcgggaatca attctattta ttacccgatc gtggccccaa tgcacaaagt tataccaatg
     2101 gcgcattggt agataacacc accagctaca ttccccgcgc ccataaaata tccatagggc
     2161 tcagcgccgc cagcagcgga agtttaccgc tgagcgtgag tgcacaatta gaatccacca
     2221 ccctttttta cagcagcagc gcgctcaatt atggcaatac agcgggtttg gcttctgcag
     2281 tgcccagcat caatacaacc cataaacagt atttttccgg ccgcagcgat aattttgccc
     2341 ccggcgccac cactaatcct aacaacgccc gtttagaccc ggagggaatc cgcatcagca
     2401 acaatggcaa aacggcttat atttcggatg agtacggccc ctatgtatac gaatttaata
     2461 ttgccacagg tgaacgcctg cgcacgttta acctgccagc ctctttaaca gcagcgcaga
     2521 gttttagtac cgaaacagcg gaaattagcg gcaataccgc tgggcgcacc accaataaag
     2581 gcatggaagg cctggccatt acacccgatg gcagtaaatt agtgggcatc atgcaggcca
     2641 ccgctctgca ggataaaagc aaaaatgtgc gtatagtggt tattgatatc gcctctggca
     2701 ccacccagga atttgcctat aaacttaccg acggcaccgg cgtaagtgaa atactggcga
     2761 ttaacaacaa tcaatttttg gtattagagc gcgacggcaa gggcctaggc gatggttctg
     2821 cggcggtggc gaaaaaactc ttccaaattg atttaaccaa cgcacaaagc gttgccggtt
     2881 tgagcggtga cctcaccagt aaagccgtag ctaaaacaca gtttttagac attacctcta
     2941 atttaattgc agcaggtttt agcgccaacc aaataccagc caaattagaa ggcattgctt
     3001 ttggtagtga tgtacagcta ggcaataccc agtaccatac acttattctg gctaacgaca
     3061 acgattacct aaatactgtg ggcggcttag ccaaccccaa ccg
//
LOCUS       DAOQGS010000497         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18651_length_3098_cov_4.015445, whole genome shotgun sequence.
ACCESSION   DAOQGS010000497 DAOQGS010000000
VERSION     DAOQGS010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18651_length_3098_cov_4.015445"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1514
                     /locus_tag="PKZ52_15260"
     CDS             219..1514
                     /locus_tag="PKZ52_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG61179.1"
                     /translation="MQTVRNPSNPMLWVPSGYFTMALMYNLLTGATVLMFSNVGMDNA
                     TATTYAGMLGLAYTIKPLFAPFLEMYRTKKFFVLLSQVILGLGLFAVAAVMQLPQFTL
                     PLLILLWILSFVGSTQDIVSDGIYVTSLDGKSQAKYTGVQSFSWNFGRLAILSGVVTA
                     SGFLHEDIFKHDPHVSGPDWVISWQIVIIAVGVWYLLMSLWHLKVMPDGSKAENPPRN
                     VQEAAATLVDAFVTFFTKKDVWLMIGFAFLYRLSYGFLNAITPLFLKDSVANGGLALT
                     NQDIGVVYGTYGIAANVIGSILGGLWIARSGLKKVLFPLCCMVNVPNITFLFLAIYQP
                     SNQPLIASLIVIEQFFFGVGSVGFMIYMMQQLAPGKYSTAHYAFGTALMGLCMMVTGM
                     ISGTLQQHMGYEHYFIFVMIATIPSFLITWYAPFHHQTK"
     gene            1747..2799
                     /locus_tag="PKZ52_15265"
     CDS             1747..2799
                     /locus_tag="PKZ52_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61180.1"
                     /translation="MRILLPLVLSGGLLAPVGALAADSPVNILGQKQTAKILEFPDMS
                     LNQMDYRGISVEFAVGGESNLRAITPIKVPLNCNGKETAITQLEVSYDLPKIFVRVHD
                     SVHNTQKYDKALPSEGKLLLGDASCDAKNDVVALEANFRSKLTDWLKGQTRQEMQGAK
                     RRVEAFVQENTVVAYKPLEITSFQFRSESGEFSNINQAYDIATKAYAMIEEKDTLSEP
                     VEGIKGAIELWEMAMGNPQINARTDAKKIKAAIAYNLSSAYWVAMNYMQASAYHKLAR
                     MLNEESGATLKLPDYQARIDRQLRNRSLSNNVPNTPVAMVTLERMGSEYIKHAEIVEL
                     TPENFKAGPRQLSAAD"
BASE COUNT          748 a          756 c          784 g          810 t
ORIGIN      
        1 gatgtgttgt tatcgttagg cctgccagtg tatcaggagt tatccgcccg gtgcgggggc
       61 acactgtgaa tcagctaaaa gggtagtaaa aaacccgccg ttggtgggtg gcgtttattt
      121 attctaaggc gttgaagtgc tattgtgccg ccgccttcca cggcctaatt cccctgtgcg
      181 ctctctttgc caaaataaaa attataggta gtcgatctat gcaaactgtc cgaaacccaa
      241 gtaatccgat gttgtgggtg ccgtctggct atttcaccat ggcgctgatg tacaacctgc
      301 tcaccggggc tactgtgctg atgtttagca acgtcgggat ggacaacgca acggccacca
      361 cctacgcggg catgctcggc cttgcctata ccatcaagcc gctgtttgcg ccctttttgg
      421 aaatgtaccg caccaaaaaa ttctttgtgc tgctctccca ggttattttg ggcttgggtt
      481 tatttgccgt ggccgcggtg atgcaactgc cgcaatttac cctgccgcta ttgatattgc
      541 tgtggatttt atcctttgtc ggcagcaccc aagatatcgt cagcgacggt atttacgtca
      601 cctcgctcga tggcaaaagc caggcgaaat acaccggggt acagagcttt agctggaact
      661 ttggccgctt ggcgatttta agtggcgtgg ttaccgccag cggtttttta catgaagata
      721 ttttcaaaca cgacccccat gtaagcggcc cggactgggt tatctcctgg cagattgtga
      781 ttattgccgt gggtgtgtgg tatttactga tgagtctgtg gcatttaaaa gtaatgcccg
      841 atggctccaa agcggaaaac ccgccgcgca atgtgcaaga ggctgcggcc accttagtgg
      901 acgccttcgt gacttttttt accaaaaaag acgtgtggct gatgatcggc ttcgcgtttt
      961 tataccgcct cagttacggt tttttaaatg cgattactcc tttgttctta aaggattccg
     1021 tcgccaacgg cggccttgcg ctgactaacc aggatatagg cgtggtatac ggcacctatg
     1081 gtattgccgc caacgttata ggctctattc tcggcggcct gtggattgcc cgctccggcc
     1141 tgaaaaaagt gctctttccg ctctgctgca tggtaaacgt gccgaatatt acctttctgt
     1201 ttttagcgat ttatcaaccc agcaaccagc ctttaattgc ctccttgatt gtaatcgagc
     1261 agtttttctt cggcgtaggt tccgtgggct ttatgattta catgatgcaa caattggcac
     1321 cgggtaaata cagcaccgcc cactacgcct ttggtaccgc tttaatgggc ctgtgcatga
     1381 tggtcaccgg catgatcagc ggcaccctgc agcaacacat ggggtatgag cactatttta
     1441 ttttcgtcat gattgccacc atcccgtcct tccttattac ctggtacgca ccgtttcacc
     1501 atcagaccaa gtaattaggc gccgcctttt aaaaaagcag cttcggctgc tttttttatg
     1561 ggctgtaaat agtgcgtcct gttcataacc aaaatacaca atttgccgat tacctagaga
     1621 tattcgttcc aattcaaagc ctgcgcaggc ggccgctaaa gtctaagctc ataaataggc
     1681 gccggattga cgtggcgcga ggtggccctg cgcgggtggt ttattggcga gtaagagagt
     1741 aaaggcatgc gaatattgtt gcccttggtt ttatcaggcg gattgttggc accggtcgga
     1801 gcattagcgg cggatagccc tgtgaatatt ttggggcaaa aacaaacggc caaaatttta
     1861 gaattccccg atatgtcgct caaccaaatg gattaccgag gcattagcgt ggaatttgcc
     1921 gtgggcggcg agagcaacct gcgagctatt accccgatta aggtgccgtt aaattgcaac
     1981 ggcaaagaaa ccgccatcac ccaattggaa gtgagttacg atttgccgaa aatttttgtg
     2041 cgcgtgcacg acagtgtgca caacacacaa aaatacgata aggccctgcc cagcgagggc
     2101 aagttgttgt taggtgacgc cagttgcgat gctaaaaatg atgtggtggc gttggaagca
     2161 aatttccgca gcaaattaac cgactggtta aaaggccaaa cccgccaaga aatgcagggg
     2221 gctaaacgcc gggtggaagc ctttgtgcag gaaaatactg tggtggccta taaacccttg
     2281 gaaattacca gttttcaatt tcgctcggaa agcggcgaat ttagcaatat caaccaagcc
     2341 tatgatatag ccaccaaggc ctatgccatg attgaggaaa aagacaccct ctcagaaccg
     2401 gtggaaggta ttaaaggcgc tattgaattg tgggaaatgg ctatgggcaa tccccaaata
     2461 aatgcccgca ccgacgctaa aaaaattaaa gccgccattg cgtataactt atccagcgcc
     2521 tattgggtgg cgatgaatta tatgcaagct tcggcctacc ataagctcgc ccgcatgtta
     2581 aatgaagagt ccggcgcgac cttaaaatta ccggattatc aagcccgcat cgaccgccaa
     2641 ttgcgcaacc gcagtttgtc caacaatgtg cccaacacac cggtggccat ggtgaccctg
     2701 gagcgcatgg gttctgaata tattaaacac gccgaaatag tggaattaac gccggagaat
     2761 tttaaagccg ggccgcggca attaagtgcg gcggattaac gcctattctg cggtttttca
     2821 ccgattgttt gcgaacattt ctgccaggga tgttcctcat ttggagatga agtctttagg
     2881 ttaaggaatg cctagccttg aaacagccgt aggggcgccc cttgtgggtg ccctttgcac
     2941 cggaaatcct gctcgatacc cgagggccgc agagtaacca caagggttgc ccctaggatt
     3001 tcgccttatt aacccggcgg caaaatagat cgtcctgatc tattttgcct tcgctgcgtt
     3061 aaacttacga aaatgcgtgg attttcttgt tttaacgc
//
LOCUS       DAOQGS010000498         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18740_length_3089_cov_3.679301, whole genome shotgun sequence.
ACCESSION   DAOQGS010000498 DAOQGS010000000
VERSION     DAOQGS010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18740_length_3089_cov_3.679301"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /gene="queA"
                     /locus_tag="PKZ52_15270"
     CDS             complement(<1..787)
                     /gene="queA"
                     /locus_tag="PKZ52_15270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNG61181.1"
                     /translation="MLRQAFAYHLPEELIAFAPAPERSTSRLLCLDGASDALAHRTFN
                     QLPQLLNSGDLLVFNNTRVIPARLFGHKASGGKVEILIERLLPGGEALAQVRASKSPA
                     PGSQILLDEQTHVQVLGRQGALYQLAFNQPPLEVLERLGHMPLPPYIERPDDEADRER
                     YQTVYAQTPGAVAAPTAGLHFDDGLLNTLKAQGIHTAFVTLHVGTGTFSPVRVDNIYE
                     HTMHSEWMQVSEEVCEQVRAAKARGNRVIAVGTTSVRCLETASR"
     gene            967..2715
                     /gene="msbA"
                     /locus_tag="PKZ52_15275"
     CDS             967..2715
                     /gene="msbA"
                     /locus_tag="PKZ52_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNG61182.1"
                     /translation="MATASADNSWQLYRRLLGYTRPYARLLIISFLGFLLYAGMETLM
                     AQLMKYFVDGLESRDKQLMWMVPLAIVAARFVHGIGSYFGNYYIARVGIHVVNDLRRD
                     LFQHLLHLPSRYYDSQNSGEIISLITYNVAQVTGSVTKAVQVGLRDGFTVLGLFVYLI
                     WVDWKLTCIFVAIAPVLGLLANTASRYFRKLSLRMQVSMGGITHITNEAIGGFRLVKS
                     FNGQTYEAARFVKACEENNRLSKKYERIAALQGPIFHLVIAINLSIILFLIMTFWQQD
                     TGTAVAYLTAAAMIAKPLRQVATINETIQKGLSAAETIFEVIDMQGEQDEYTAPLTVT
                     QGAIEFREVNFSYTEHKQALRDINFTVLPGQTIALVGRSGSGKSTLSHLLLRLYELSQ
                     GSILIDGTTINRVSLHSLRQNIALVNQHTVLFNDTVARNIAYGLDDKDIDFARVQKAA
                     ELAQAKEFILALPEGFNTLIGEDGDRLSGGQRQRLAIARAIYKDAPILILDEATSALD
                     NEAEKAIQIALDALAQGRTTIVIAHRLSTIEAADKIIVMEQGAIVETGTHAELMAANG
                     AYTNLHRSAVNGVTPE"
     gene            2712..>3089
                     /locus_tag="PKZ52_15280"
     CDS             2712..>3089
                     /locus_tag="PKZ52_15280"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNG61183.1"
                     /translation="MIARRLYTLIWYLALPIILGRLIWRSIKAPGYRSHIAERLGFIT
                     PITSSKPCLWIHAVSLGESIAATPLIKALLQSGEFHLLITNTTPTGRARIAQEFGNQV
                     SQAWAPYDIPACIERFLTSAKPCG"
BASE COUNT          719 a          800 c          815 g          755 t
ORIGIN      
        1 tgcggcttgc ggtttctaaa cagcgcaccg aggttgtgcc tacggcaatt acgcggttgc
       61 cacgggcttt ggctgcccgc acttgctcac acacctcttc actcacttgc atccattcac
      121 tgtgcatggt gtgttcataa atattatcca ctcgcacggg ggagaaggtg cccgtcccca
      181 cgtgcaaggt gacaaatgcg gtatggatgc cctgggcttt aagcgtgttc agtagcccat
      241 catcaaaatg caggcctgcc gtgggagcgg ccacggcacc gggggtttgc gcgtacacag
      301 tttggtagcg ctcgcgatcg gcctcatcat ccggccgctc aatatagggc ggtagtggca
      361 tatggcccag acgctccagc acctcaaggg ggggctggtt aaacgccaat tgataaaggg
      421 ctccctgccg cccgagcacc tgcacatggg tttgttcatc taacaggatt tggctgccag
      481 gggcggggct tttgctggca cgcacttggg ctagggcctc accgccgggc aacaggcgct
      541 caatgaggat ttccaccttg ccgccgctgg ctttatggcc gaataaacgg gcgggaatca
      601 cacgagtgtt gttaaacacc aataaatcgc cggagtttaa caattgaggc aattgattaa
      661 aggtgcggtg ggcgagtgcg tcgctcgctc catctaaaca cagcaggcgg ctggtgctgc
      721 gctcaggggc tggagcaaag gcgatgagct cctcgggcaa atggtaggca aatgcctggc
      781 gtaacataat cggtctcaaa taaaacgcca cttaaggcat atccgcgcaa agtgcggcca
      841 gaaaaaaccg cgcagggtac tggaatttag ccgcctgcgc tacgacaggc tcgcaacaag
      901 acggcaggcg gcaaatcagc gataatcgcg ccctttcgtt ttccgctcta caaacttgag
      961 accaccatgg ccactgcctc tgctgataat tcctggcaac tctaccgccg tttattaggc
     1021 tatacccgcc cctacgctcg ccttttgatt attagttttc tcggcttttt actctacgcc
     1081 gggatggaaa cactcatggc gcagctgatg aaatactttg tggacgggtt agaaagccgc
     1141 gataaacaac tgatgtggat ggtgcccctg gccattgtgg ccgctcgttt tgtacacggc
     1201 atcggctcct attttggcaa ctattacatc gcccgggtgg gcattcatgt ggtgaatgat
     1261 ttacggcggg atttattcca acatttattg cacctgccca gccgctatta cgacagccaa
     1321 aattccgggg aaattatttc ccttatcacc tataacgtgg cccaggtaac tggctcggtg
     1381 accaaagcgg tacaggtggg cctgcgggat ggttttaccg tgctgggttt gtttgtgtat
     1441 ttaatttggg tggattggaa actcacctgt atttttgtcg ctatagcgcc agtgctgggt
     1501 ttattggcca acaccgccag ccggtatttt cgcaaattaa gcctgcgcat gcaggtgagc
     1561 atgggcggca ttacacacat taccaacgag gccatcggcg ggtttcgttt ggtgaaaagt
     1621 tttaacggcc agacgtatga agcggcgcgc tttgtaaaag cctgcgagga aaacaaccgt
     1681 ctgagcaaaa aatacgagcg tatcgccgcc ctgcaggggc cgattttcca tctggtgatc
     1741 gccattaatt tatccatcat tttgttttta attatgacct tttggcagca ggataccggt
     1801 acggctgtgg cctatttaac ggcggctgcc atgatcgcca aacctctgcg gcaagttgcc
     1861 actattaatg aaaccattca aaaagggctt tcagccgcgg aaacaatttt tgaagtgatc
     1921 gatatgcaag gcgaacagga tgaatacacg gcgccgctga cagtaaccca gggcgccatt
     1981 gaatttcgcg aggtgaattt tagctacaca gaacacaaac aggctttgcg ggatattaat
     2041 tttaccgtat tgcctgggca aaccattgcc ctggtgggcc gctccggcag cggtaaaagc
     2101 accctctccc atttgttgtt gcgtttatac gagcttagcc aaggcagtat tttaattgac
     2161 ggcactacca ttaaccgcgt cagcctccat agcctacggc aaaatatcgc tttggtgaat
     2221 caacacacgg tgttatttaa cgatacggtt gcccgcaata ttgcctatgg tttggatgat
     2281 aaagacatag attttgcgcg ggtgcaaaag gccgccgaat tagcccaggc taaagaattt
     2341 attctcgccc tgccagaggg ttttaatacg ctgattggcg aggatggcga tagactctcc
     2401 ggcggccagc gccagcgttt agccatagca cgggccattt ataaagatgc accgatttta
     2461 attctcgatg aagccacttc cgccctggat aacgaagcgg aaaaagctat tcaaattgcc
     2521 ctagatgccc tcgcccaagg ccgcaccacc atcgtcattg cccaccgcct aagcaccatt
     2581 gaagcggcgg ataaaattat tgtgatggag caaggcgcga tagtggaaac cggcacccac
     2641 gctgaattaa tggccgcgaa tggcgcttat actaatttgc atcgttcggc tgtaaatgga
     2701 gttacgccgg aatgattgcg cggcgcctgt atacgcttat ttggtatttg gccctgccga
     2761 ttattctcgg ccgtttaatt tggcgcagca ttaaagcgcc gggttatcgc agccatattg
     2821 ctgagcgctt agggtttata acgccgatta catcgagtaa accctgcctg tggattcacg
     2881 cggtgtcctt aggggagagc attgcggcaa cgcctttaat taaggctcta ttgcagtctg
     2941 gggaatttca tttattaatt accaatacaa cccccaccgg gcgggcccgc attgcgcagg
     3001 aatttggcaa ccaggttagc caagcctggg ccccttatga tattcccgcc tgtattgaac
     3061 gctttttaac aagcgccaaa ccctgcggc
//
LOCUS       DAOQGS010000499         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18756_length_3087_cov_4.623351, whole genome shotgun sequence.
ACCESSION   DAOQGS010000499 DAOQGS010000000
VERSION     DAOQGS010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18756_length_3087_cov_4.623351"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKZ52_15285"
     CDS             <1..230
                     /locus_tag="PKZ52_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNG61184.1"
                     /translation="CAFYFAHPYSSWQRGSNENTNGLLRQYYPKNTNFRLVDDEQLKR
                     VINELNERPRKVLGYRTPMDLMNDQLAATAA"
     gene            complement(227..436)
                     /locus_tag="PKZ52_15290"
     CDS             complement(227..436)
                     /locus_tag="PKZ52_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61185.1"
                     /translation="MKKQIVSLVAVVALAGWVSAHAEPTTTKLKIVKVRPYNNASGGP
                     GDVYIQTDRTGLCNTADSTPKCIIH"
     gene            936..1145
                     /locus_tag="PKZ52_15295"
     CDS             936..1145
                     /locus_tag="PKZ52_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61186.1"
                     /translation="MNRTDIQTREQRAKQSNFHISPQQFYDLGLTLWAMQQALRSQIQ
                     VTIQKLKSPNHNQIQQVRNTNDHVA"
     gene            complement(1320..1649)
                     /locus_tag="PKZ52_15300"
     CDS             complement(1320..1649)
                     /locus_tag="PKZ52_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61187.1"
                     /translation="MSKYFWKMLPIAAACISGFSHAGSVSGTISQVMAGQDNWYGVRF
                     YLNITNNQTNGECNPSFVYVEPEAGSGYQAKVSVFLAAYLAGKTVDMTVTAGRGGYCK
                     IFEGVMR"
     gene            complement(1918..2241)
                     /locus_tag="PKZ52_15305"
     CDS             complement(1918..2241)
                     /locus_tag="PKZ52_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61188.1"
                     /translation="MKYLLLAMTFISVSSYAAGGTGLRKILGIGCHNGGDPVCYVTIE
                     GAPVGPAGCNSTSIRWDSSLAGGKNQFALLTAAFMAGKPVDLFIMDTCFPLQPGYPTF
                     SFSNL"
     gene            complement(2679..3005)
                     /locus_tag="PKZ52_15310"
     CDS             complement(2679..3005)
                     /locus_tag="PKZ52_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61189.1"
                     /translation="MKRLFTLGFLIFFTMSAHAWLGPSVQIVDIYFEGLANDQRMVIV
                     VDRNIHSCGWNSAAQIDRSVVGDQAFQTYSSVILSAFYASPKVDLAVIGCTGDRARVN
                     AVRILK"
BASE COUNT          975 a          781 c          552 g          779 t
ORIGIN      
        1 attgtgcatt ttattttgcc catccttatt cgtcttggca gcgcggctcg aacgaaaaca
       61 ccaacggttt gttgcggcag tattacccga aaaataccaa ctttaggttg gttgatgacg
      121 agcaactaaa acgggtaatc aatgaattaa atgaacgccc acgcaaggtt ttagggtata
      181 gaacaccgat ggacttaatg aatgaccaac tggcggctac tgccgcttaa tgaatgatgc
      241 acttcggggt tgaatccgcc gtattgcaaa ggccggttct atccgtctgg atatatacat
      301 cccctgggcc acctgaggcg ttgttataag gccgaacttt gacaattttt aattttgtag
      361 ttgtaggttc tgcatgagca gaaacccaac cagcaagtgc aacaactgcc acgaggctta
      421 caatctgttt tttcatacta cacacctttt tacgttcgcc cgcttatact tatagcagcg
      481 ggattaaaaa tacaaaagaa acttcaactt catacatttt tagtaagata ctagcgagat
      541 cgccctagtg aacaacaacc gaaacaccta tggtaaaggc gtggccagaa tttaattcac
      601 ttaaccacaa ccccacttta ctagcaaaaa aattattaaa aaccctagcc agtttaaagc
      661 aactcaatat gcaccaatga acatgacgta gagtcaaaaa cacctctgac ctgctcctca
      721 tacccaacta gcaccgggct tttttgcgct ttagccatca gcaccatact cagtgcgcta
      781 ctgtcctcaa ttttaatgta taaggagtta caaccgcccg agagggtctg atttaccttg
      841 aaaattgcat acttgcgagc agccactgtt gcctgctcac ttgtataata tcggactgat
      901 tcgattaaca aattactcct ttccgcagca tgtacatgaa tcgaactgac atacaaaccc
      961 gcgaacaacg cgctaagcaa tctaattttc atatttctcc ccaacaattt tacgacctcg
     1021 gcttaacctt gtgggcgatg cagcaagcct tgcgctcaca aattcaagta accatccaaa
     1081 agctaaaatc acctaatcac aatcaaattc agcaagtacg taatacaaat gaccacgttg
     1141 cttagtcatg ttagccgaag gcgtagcccg cgcgtttaat tcaaattcag gtacatacca
     1201 aaaaagcaaa atgatttcct caagttcagg cacgaaaccg cgcgcgccaa ttgacccgcc
     1261 ctacacggcc taaaacacat atgaaaccac ccttcatttt ttatgactgc agcaataggt
     1321 taacgcatca ccccttcaaa aatcttacaa tatcctccgc gccctgctgt cacagtcata
     1381 tcaacagttt tacctgccaa ataggcagcc aaaaaaacag atactttcgc ctgataaccc
     1441 gaccctgctt ccggttccac atagacgaag gaggggttgc actccccatt tgtttggtta
     1501 ttagtaatgt ttaaataaaa acgcacacca taccaattat cttggccggc cattacctgc
     1561 gatatagttc cactcactga acccgcgtgg gaaaaaccag aaatacaggc agccgctata
     1621 ggcagcatct tccaaaagta tttgctcatt taaatttcct ccatagataa actaatttaa
     1681 cgcctactta gatgtgtgaa tttccaattg gaagcccgca ttaaccaatg cgcggctgca
     1741 ctcagccgca accccaaaca agaccattta ttttgttttc cggcgtgcgg cagcggcgat
     1801 ttatcaaacg cacagccatg catttataat taatcctcaa accaaaaaac aacatccaat
     1861 tgcacgagac aaaaccgcgc gggttacact tcgcttacaa gactgcgttt caggtggcta
     1921 aaggttagaa aacgaaaatg ttggataacc tggctgcaaa ggaaaacagg tgtccataat
     1981 aaacagatca actggctttc ctgccataaa tgccgctgtc aacaacgcga actgattctt
     2041 accccctgcc aaactgctat cccagcgaat tgaggtactg ttacatccgg ctggaccgac
     2101 aggggcaccc tcaatcgtta cataacaaac cggatcacct ccattatgac aaccaatacc
     2161 cagaattttt ctcaatcctg ttcctcctgc agcataacta gacacagaaa tgaaagtcat
     2221 tgccaatagt aaatatttca cagcacttct ccaacgttta ataaaaattc attgccgctt
     2281 ctcattcgat cagccaatta ctatcaacca taattactgc tggcgctggg caactccgcg
     2341 cagatattca atattccgta aagacaatac aaaaattcac acccacacaa aacggcacaa
     2401 gcattcggaa ttaaaactca acaaccgccc agtcaatctt atatctttca atacaattaa
     2461 ccgagatatg gcgggaacat atggaacctt cccgtgttct ccgcgacata aaaaagtggc
     2521 gtcggccaag ttagctgaag gtgtaaacca cggattcaat tcaaattcat gcacaatcca
     2581 ataaaggtat aatcattact tcagatgaag aggagaaacc gcgcgagtca aataaaccac
     2641 actacgctga tgaacctttg gcattcaatc tttttaaatc attttaatat tcgtacggca
     2701 tttactcttg ctctatcacc ggtgcaacca atcacggcaa gatcaacttt tggcgaagca
     2761 tagaatgcgc ttaatatgac acttgagtag gtttgaaatg cttgatcccc caccacgctg
     2821 cgatcaattt gtgcagctga gttccagcca cagctgtgaa tatttctatc caccacaatt
     2881 accatccgct gatcgttggc taggccttca aaatagatat cgacaatctg aaccgatggg
     2941 cctagccacg catgagcgga cattgtaaag aatataagaa agccgagagt aaaaagtctt
     3001 ttcatttttt aacaccaaaa tttgttagtg gtggggtgga ttaggcgaag accaaacccg
     3061 agtgaaattc ataatcaagc aaataac
//
LOCUS       DAOQGS010000500         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18770_length_3086_cov_4.553283, whole genome shotgun sequence.
ACCESSION   DAOQGS010000500 DAOQGS010000000
VERSION     DAOQGS010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18770_length_3086_cov_4.553283"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKZ52_15315"
     CDS             complement(<1..182)
                     /locus_tag="PKZ52_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61190.1"
                     /translation="MDKRMFYLWGLVIISLASVLGVFIWLPPIAQTVAYHQFADERSF
                     LSISHFWNVVSNLMFAV"
     gene            complement(256..1125)
                     /locus_tag="PKZ52_15320"
     CDS             complement(256..1125)
                     /locus_tag="PKZ52_15320"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNG61191.1"
                     /translation="MSQVSIALDIHANLGECPRWDEETQLLYWVDINNKQLHRFNPST
                     GQDEFLTFEQEIGCFALRQQGGFVLGMRNGFYVLDGWNTDIRFITDPEAHLEKNRFND
                     GRVDALGRLWAGSVYPPKDYNGANIYSLSPDGVATHQVGNLLTANGIAFSPDSRTLYY
                     ADTPSHAIMAADFDLAAGTISNTRVFHQFPHGQGRPDGGCVDSEGCYWTALYEGGRVV
                     RLSPAGDILQEIPVPARCPTMPAFGDGDLKTLYITSVSSRPEAELAEYPHSGAIFKVR
                     VDVPGRVENRFLG"
     gene            complement(1605..2144)
                     /locus_tag="PKZ52_15325"
     CDS             complement(1605..2144)
                     /locus_tag="PKZ52_15325"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNG61192.1"
                     /translation="MERRQYFRINDTLGFSYRLIGESKKEDTFWSSLAQMDEQINQLL
                     GVVANKQPSLGKLLGLMNQKLERIASQLMLDGELMQKMASRVGEVNISACGIGFSHPQ
                     AVAAKSQIALKLHLLPSDTRVAAEGKVVDCATQPDGLYYWRIKFTRMAEAERDLLIRH
                     LIERQRQQIKESRSQNDGV"
     gene            complement(2153..3046)
                     /locus_tag="PKZ52_15330"
     CDS             complement(2153..3046)
                     /locus_tag="PKZ52_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNG61193.1"
                     /translation="MYFHDENDERPPVHTKAPAWVITFADLMSLLMCFFVLLLAYSEM
                     DAKKFAQVASSMQGSLGGGTVPQQGEGLIPMPVPQAESADTPPEQPSEEDQEAKALME
                     KLNQTLAQTQADAQQLAQRLQGQMERGELEIETDGPNIIVRLREQGSFDSGSADLNPQ
                     AQATLATIESVLQSKQGRILVLGHTDDVPIKTPRFRSNWELSAVRAVAVADALMSSGL
                     LARARFEVTGFADTHPLTGNDTPQGRARNRRVEIVIAQDLNPNLTEQERLLLERTSSN
                     PLLKPRQVRPKPKGALPEHIF"
BASE COUNT          667 a          857 c          882 g          680 t
ORIGIN      
        1 acagcaaaca tcaggtttga aacgacgttc caaaaatgac tgattgagag aaagctacgc
       61 tcgtcggcga attgatgata tgcgaccgtt tgagcgatgg ggggcaacca gataaatacg
      121 cccaacacac tcgccaggct aataatcacc agaccccaca ggtaaaacat ccttttatcc
      181 ataccacaca cctaaattaa ctgagtctct gggaaatact ggtcaattgg cttagcgccg
      241 aggcaaccga aacctctagc ctaaaaagcg attttccact cggcccggca catctacccg
      301 cactttaaaa atcgcccctg agtgggggta ttcggccagc tcggcctcgg gacggctgct
      361 aacgctggtg atataaaggg tttttaaatc cccgtcacca aaggcgggca tggtggggca
      421 gcgggcgggt acggggattt cctgcagtat gtcaccggct gggcttaggc gcaccacccg
      481 gccgccctca tatagggccg tccaataaca gccttctgaa tccacacagc cgccgtcggg
      541 gcggccctgg ccgtggggga attggtggaa tacacgggta ttgctgatgg tgccggctgc
      601 caggtcaaaa tccgcggcca tgatggcgtg gctgggggta tcggcataat aaagggtgcg
      661 gctgtcgggg ctgaaggcga tgccgttagc ggtgagtagg ttccccacct ggtgggtggc
      721 caccccgtcg gggctcaacg aatagatatt cgcgccgttg tagtccttcg gcggatacac
      781 actgcccgcc cagaggcggc cgagggcatc cacgcggccg tcgttaaagc ggtttttctc
      841 caggtgggcc tctggatccg tgataaatcg aatatcggta ttccagccgt ccaacacata
      901 aaagccgttg cgcatgccca atacaaaacc tccctgctgg cgcagagcga agcagccaat
      961 ttcctgctcg aaggttaaaa actcatcctg acccgtggag gggttaaagc ggtgcagctg
     1021 tttattattg atatccaccc aatacagcaa ctgggtttct tcatcccagc gggggcactc
     1081 ccccaaattg gcgtggatat ctaaggcaat gctcacctgg ctcatgtgta ctccaacagg
     1141 ttttagtcaa aatggaaata atctaaatga tggcacagca acccgctgaa ttaaacgctc
     1201 aaaacgggat tcgccgaatt tgatacgcag aaaagaaaaa ccccgccttg ggcaggcagg
     1261 gttttaggag gtttgggcgt gggcgaccta gggtcgatgc aggcgtggag cctaaagcgt
     1321 gtactgagag gggcactgcc ttgcaacggg tatcgcgtgt tgcggtgcga tggtaccccg
     1381 ccggagcagc tctgccaagg acaaaataag ccttggggca aaaaaaaacc gcaacctcat
     1441 gtcgcggcca attctcacag tatgttaatc cttaaccctc ctcggtgatc ccttaatctt
     1501 ccttaaagtg ttcctttgcg ctggttccat tcaagtggga gcagtttagt cgccgggcta
     1561 aatttaccaa gtggcgttta acctcaatgc gtgtgagata tttcctacac gccatcgttt
     1621 tgtgaccggg actcctttat ttgttggcgc tggcgctcaa ttaggtggcg aattaagaga
     1681 tcccgctccg cctccgccat acgggtaaat ttaatgcgcc agtaatagag gccatctggc
     1741 tgggtcgcac agtcaacaac cttcccctca gcggccaccc gggtatcgct gggcaataaa
     1801 tgtagcttaa gggcgatctg gcttttcgcc gccacggctt gcgggtggct aaagcctatg
     1861 ccgcaggcac tgatgttcac ctcgcccacc cggctggcca ttttttgcat caattcgcca
     1921 tcgagcatca actggctggc gatgcgctca agtttttgat tcatcagccc cagcagtttg
     1981 ccaagcgagg gctgcttgtt ggccacaacg cccaacagct ggttgatctg ttcgtccatt
     2041 tgcgccaggg aggaccaaaa ggtgtcttct tttttgctct cgccgattaa ccggtaggag
     2101 aaccctaggg tgtcgttaat gcggaagtat tgcctgcgct ccacggtgac tcctagaaaa
     2161 tatgctcagg cagtgcaccc ttgggtttag ggcgcacctg gcgaggcttt aacagcgggt
     2221 tgctactggt gcgctccaac aataggcgct cctgttcggt caaattgggg ttgaggtctt
     2281 gggctataac gatttccacc cggcggttgc gcgcccgccc ctggggggtg tcgttgcccg
     2341 tgagtgggtg ggtgtcggca aaaccggtga cttcaaagcg ggcgcgcgcc agcaggcccg
     2401 agctcatcag cgcatccgcc accgccaccg cacgcacggc ggagagctcc caattagagc
     2461 gaaaacgggg ggttttgatg ggtacatcat ccgtatggcc caagaccaaa atacggccct
     2521 gcttgctctg caaaacactc tcgatagtag cgagggtggc ctgggcctgg gggttaagat
     2581 cggcggaacc cgaatcaaaa ctgccctgct cccgcaggcg cacgataatg ttagggccgt
     2641 cggtttcgat ctccagctcg ccccgctcca tctgcccctg caggcgctga gccagctgct
     2701 gggcatccgc ttgggtttga gcgagggtct ggttgagttt ttccatcagg gccttggctt
     2761 cttgatcctc ctcggagggc tgctcaggcg gtgtgtcggc ggattccgcc tggggcacag
     2821 gcatggggat taagccctcg ccctgctgcg gaacggtgcc accgcccagg ctgccctgca
     2881 tggagctggc tacctgggcg aattttttcg catccatctc gctgtaggcc agcaagagca
     2941 caaaaaaaca catcagcagg gacattaaat ccgcaaaggt gatgacccag gcgggggctt
     3001 tggtgtggac tggcgggcgc tcgtcgtttt catcgtggaa atacacggct cacctcttac
     3061 ccttgcgctg gccttccggc aaatag
//
LOCUS       DAOQGS010000501         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_18790_length_3084_cov_4.667547, whole genome shotgun sequence.
ACCESSION   DAOQGS010000501 DAOQGS010000000
VERSION     DAOQGS010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18790_length_3084_cov_4.667547"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKZ52_15335"
     CDS             complement(<1..503)
                     /locus_tag="PKZ52_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HNG61194.1"
                     /translation="MSDLFDEAVEELQTFVDWVRFGASRFTEANLFYGHGTDNPWDDA
                     VALTLAAVHTPFDRLGQIAHAKLTRSERVHLARLFQRRINERMPVPYLTGEAYFAGLK
                     FKVTPDVLIPRSPLGEKIEEYFEPYLPQAPERILDLCTGSGCIGIACALAFEAAGVVL
                     SDISAAA"
     gene            complement(658..843)
                     /locus_tag="PKZ52_15340"
     CDS             complement(658..843)
                     /locus_tag="PKZ52_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61195.1"
                     /translation="MGEVIPFKRPTAWQKHKGKTLCREGFHKWVVAKEKQFDVKQGKL
                     VTLYRCARCGETKVQTH"
     gene            complement(868..2934)
                     /locus_tag="PKZ52_15345"
     CDS             complement(868..2934)
                     /locus_tag="PKZ52_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNG61196.1"
                     /translation="MRRLKISAKLTLMCVVTTAIALFCVLLGFIVQDLRLVKALKDEQ
                     TNTELTLLSNNLSALVAQDDTSAIHVVIRHSLSRHSIVAIWVQDQDGSVIDAYPEHIK
                     TYGDFTQFPTRHPNDYSMRMVQRELLAGNLPVGDVFVWVSNSDVESRGEQLRYYALMA
                     FGFALVLAVIIGWLTQKIISDPLKALQKLSQSVIESGDYSLRSSLHTQDEIGQLSSAI
                     DRMLSQIQQRDAMLEGQVSQRTQELQQLADDFRYRAMHDILTGLPNRAFLAEEFPRAV
                     AHARRVNKSFALLLLDLDNFKTINDSFGHDVGDELLKQVAVRMRSVLRGEDMICRLGG
                     DEFVVLVEDVEEAEHVRAVGGNLLQTLHSEMWLGGRSMRISVSIGAALYPDNGRELSD
                     LNRAADIAMYRAKDTGKNQIILYSPHMEQKALYRMLVQNDLRDGMVRDELIVYFQPQV
                     DARLHRLHGCEALVRWQHPLEGFLVPQTFLSYAEEHGLIRHLDYYVLRKACEACMRWR
                     KELGADIPVSVNFSGAHFHSRALLASIKEILNTSGLPAHLLMVEITEGVLIEDPIMAR
                     QLIAEIRELGVRIGLDDFGAGYSTLNYLRSLEVDTVKLDRSFCQSVHSDETEYRMTKG
                     IISLARDFGVELIAEGVENADQLHVLQELGCDYIQGYYFARPLAESEFVAWYQTFKRQ
                     PQLDFV"
BASE COUNT          814 a          869 c          693 g          708 t
ORIGIN      
        1 aaagccgccg ccgatatatc gctaagcacc accccagccg cttcaaatgc caatgcgcac
       61 gcaataccaa tgcagcccga gccagtgcac agatccaaaa tgcgctcggg ggcttgcggc
      121 aaatagggtt caaaatattc ctcgattttt tcacccaacg gtgagcgcgg aatcagtacg
      181 tccggcgtaa ctttgaattt aagcccggca aaataggcct cgccggttaa ataaggcaca
      241 ggcatgcgct cattaatacg gcgctgaaat agccgcgcca aatgcacgcg ctcactgcgg
      301 gtaagtttgg catgggcgat ttggcctaag cggtcaaagg gcgtatgcac cgccgcaagg
      361 gtcagcgcca ccgcatcatc ccaaggatta tcggtgccgt ggccgtagaa taaattagct
      421 tccgtgaagc ggctggcgcc gaaacgaacc cagtctacaa aggtctgcag ttcctctacg
      481 gcctcgtcaa ataaatcgct catcacccgc cccgacagct acaaaagccg cgcagtttac
      541 ctgatctggc ccaccaaggg cacccaggga accctcaagc agctttgctt aaaatgctca
      601 ggcggcgacc ggccggtacg atttgctcaa cgctactagt gggcttgcat cttaaattca
      661 gtgggtttgc actttggttt caccgcagcg ggcgcagcga tacagggtta caagcttgcc
      721 ctgtttgacg tcgaactgtt tctccttcgc caccacccat ttgtgaaaac cctcgcggca
      781 gagggttttg cccttgtgtt tttgccaggc cgtggggcgc ttaaagggga ttacctcacc
      841 catggggcgc ccgcctgtat gcggcaatca aacaaagtcc agctgcggct gccgtttaaa
      901 cgtctggtac caagccacaa attcgctctc cgccagaggc ctggcgaaat aatagccttg
      961 aatatagtca cagcccaatt cctgtaaaac atgtaattga tcggcatttt ctaccccttc
     1021 agcaatcagc tccacaccaa aatcccgcgc cagggagata attcctttgg tcatccggta
     1081 ttcggtttca tcgctgtgca ccgactgaca aaaagaccga tccaatttta cggtatccac
     1141 ttccaagctg cgcaaataat tgagcgttga ataacccgca ccaaaatcat ctaaacctat
     1201 gcgcacaccc agctcgcgaa tttcggcaat taactgacgc gccataatcg ggtcttcaat
     1261 taacacgccc tctgtaattt ccaccatcaa taaatgcgcg ggcaggcccg aggtgttgag
     1321 aatttcctta atactggcta aaagtgcccg cgagtgaaaa tgtgcgccgg aaaaattcac
     1381 gctcacagga atatcggcac ccaactcctt gcgccagcgc atgcaggctt cacaggcttt
     1441 gcgcaacaca taataatcca agtggcgaat caggccgtgt tcttctgcat agcttaaaaa
     1501 agtctgcggc actaaaaaac cttccagcgg atgctgccag cgcaccaagg cttcgcagcc
     1561 gtgcaggcgg tgcaagcggg catccacctg gggttgaaaa taaacaatta attcatcgcg
     1621 caccatgcca tcgcgcaaat cattttgcac cagcatacgg taaagcgcct tttgctccat
     1681 gtgggggcta tagagaataa tttggttttt ccccgtatcc ttagcgcggt acatcgcaat
     1741 atccgcagcc cggttaagat cggataactc gcggccatta tccggataca aggcggcgcc
     1801 aatactcacg ctaatacgca tggagcgccc acccagccac atttccgagt gcagggtttg
     1861 caataaattg ccgcccacag cccgcacgtg ttccgcttct tccacatctt ctaccaacac
     1921 cacaaattca tcaccgccca ggcgacaaat catatcctca ccgcgcagta cgctgcgcat
     1981 acgcacggca acctgtttta ataattcatc gccaacgtcg tgcccaaaac tgtcgttaat
     2041 ggttttaaaa ttatctaaat ccagtaataa cagcgcaaaa cttttattga cgcgtcttgc
     2101 atgggccacg gcgcgcggaa attcttctgc taaaaacgca cgatttggca agccggtcaa
     2161 aatatcgtgc atggcgcggt agcgaaaatc atctgccaat tgttgcagct cttgggtgcg
     2221 ctgggatacc tgcccttcca acatggcgtc gcgctgttga atttggctga gcatgcggtc
     2281 gatggcgctg gataactggc cgatttcatc ctgggtatgc aaacttgaac gcaggctgta
     2341 atcgccagat tcaataacgc tctgggacaa tttctgtagt gcttttaagg ggtccgaaat
     2401 tattttttgc gtcagccaac cgataattac cgccagcact aacgcaaaac caaaagccat
     2461 taaggcataa taacgcagtt gttcgccgcg gctttccaca tccgaattgc tcacccaaac
     2521 aaatacatcc cccacgggta agttacccgc caagagttcg cgctgcacca tgcgcatgga
     2581 ataatcattc ggatgacgag tgggaaattg cgtaaaatcg ccataggttt tgatgtgctc
     2641 gggataggca tcaatcacac taccgtcctg atcctgcacc caaattgcca ctatgctgtg
     2701 gcggcttaaa ctgtggcgga taacaacatg gatcgccgaa gtatcatcct gtgctaccaa
     2761 tgcggataaa ttattactca aaagcgtaag ctcagtgttc gtctgctcat ctttcagcgc
     2821 ttttaccagc cgcagatcct gcacaataaa tccaagcaaa acacaaaaca acgcaatggc
     2881 agtggttact acacacatca aggtcagttt tgcgctgatt tttaaacggc gcatagcagc
     2941 taacacctta tttacactcc acgcgtcaaa aatgattaat acaaacgcta aaaaatgatg
     3001 ctaattatct aacagccttg aacctaaata ccgcagttga actctactac ggccgcctaa
     3061 tgcttgaatc tgcacgcttt tttg
//
LOCUS       DAOQGS010000502         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19012_length_3063_cov_3.913564, whole genome shotgun sequence.
ACCESSION   DAOQGS010000502 DAOQGS010000000
VERSION     DAOQGS010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19012_length_3063_cov_3.913564"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="odhB"
                     /locus_tag="PKZ52_15350"
     CDS             <1..1051
                     /gene="odhB"
                     /locus_tag="PKZ52_15350"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNG61197.1"
                     /translation="ISEILKQQGDVVLSNEVIAYFVAGAGAGAGAGAAAAPAAAPAAA
                     APAAASASAPVNPAAKKLAAEKGLDTASIVGSGKDGRVLKEDVLAASAKPAAAPAAKP
                     AAAAVAAPVVAEGFRVEKRVPMTRLRSRIAERLLDAAQNTAMLTTFNEVNMGPVMALR
                     NKYKDQFEKAHQGTRLGFMSFFVKAAVEALRRIPAVNASIDGNDIVYHGYQDIGVAVS
                     TEKGLVVPVLRNAEHMGLADIENGIRDFGLRARDGKLGIEEMTGGTFTITNGGVFGSL
                     MSTPILNPPQAAILGMHKIQERPMAVNGKVEILPMMYLALSYDHRLIDGKEAVQFLVA
                     IKDMIEDPARILLDI"
     gene            1130..>1370
                     /locus_tag="PKZ52_15355"
     CDS             1130..>1370
                     /locus_tag="PKZ52_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG61198.1"
                     /translation="MSEKFDVIVIGSGPAGYVAAIRAAQLGLKTACIEKWKNSDGKGV
                     NGGTCLNVGCIPSKALLDSSYKYHEAAHALAGHGIA"
     assembly_gap    1371..1412
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1413..2599
                     /locus_tag="PKZ52_15360"
     CDS             <1413..2599
                     /locus_tag="PKZ52_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG61199.1"
                     /translation="AMIARKETIVKQLTGGIAGLFLANKVTALYGTGKLLAGRKVLFT
                     DFEGKEQTLEADNVILASGSIPVNIPVAPVDNDVIVDSTGALAFDRVPGRLGVIGAGV
                     IGLELGSVWNRLGSKVVCLEAMESFLALMDQQIAKETQKILTKQGLDIRLSSRVTGTQ
                     IKTVGAEKEVEVTYIDKEGKEVKETFDKLIVCVGRRPFTQGLLAEDSGVKLDERGFIY
                     VNDLCSTSAPGVWAIGDVVRGPMLAHKGSEEGVMVAERIAGHKAVLNYDIIPSVVYTH
                     PEVAAVGKTEEQLKAAGENYKVGTFPFVASGRALAADEKDGLVKIIAHADTDRILGAH
                     IVGPSAADLVQQVAIAMEFGSSAEDLGMMVFGHPTLSEAVHEAALAVNGHAIHIANRK
                     KK"
     gene            2771..>3063
                     /locus_tag="PKZ52_15365"
     CDS             2771..>3063
                     /locus_tag="PKZ52_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNG61200.1"
                     /translation="MNLHEYQAKQLFAEYGLPVSKGVAAATPAEAAAAADTIGGNLWV
                     VKAQVHAGGRGKAGGVKLVSTKAEIEEFASKWLGKNLVTYQTDAKGQPVSRILV"
BASE COUNT          685 a          827 c          838 g          671 t
ORIGIN      
        1 cattagcgaa atccttaagc agcagggcga tgttgtcttg agcaacgaag ttatcgccta
       61 tttcgtcgcc ggtgccggtg ccggtgccgg tgccggtgcc gccgccgcgc cagcagccgc
      121 tcctgccgcc gcagcgcccg ccgctgccag cgccagcgca cctgtcaacc cagcggcgaa
      181 aaaattggcc gctgaaaaag gcctggatac cgcaagtatc gtaggctcgg gtaaggatgg
      241 ccgggtgtta aaagaagatg tgctggccgc atcggctaaa ccagccgccg cccccgccgc
      301 caaacctgct gctgccgccg tggctgcacc ggttgtggcc gaaggcttcc gagttgaaaa
      361 acgcgtaccc atgacccgcc tgcgctcgcg catcgccgag cgcctgctcg atgccgcgca
      421 aaacaccgcc atgctcacca cctttaacga ggtgaacatg gggccggtta tggccctgcg
      481 caacaaatat aaagaccagt tcgaaaaagc ccaccagggt acccgtttgg gctttatgag
      541 cttcttcgtt aaagcggctg tggaggccct gcgccgtatt ccggctgtaa acgcttccat
      601 cgatggcaac gacatcgtct accacggcta tcaagatata ggcgtggccg tgtctaccga
      661 gaaaggctta gttgtgcctg tgctgcgcaa cgccgaacac atgggccttg ccgatataga
      721 aaacggtatc cgcgattttg gcctgcgtgc ccgcgacggc aaattgggca tcgaagagat
      781 gaccggtggc accttcacca tcaccaatgg tggtgtgttc ggctccttga tgtccacgcc
      841 gattctcaat ccgccccaag ccgctatttt gggtatgcat aaaatccaag agcgccccat
      901 ggcggtgaat ggcaaggtgg aaatcctgcc catgatgtac ctggccctgt cctatgacca
      961 ccgtttgatc gatggcaaag aagccgtgca attcctggtt gccattaaag atatgatcga
     1021 agatcctgca cgcattctgt tggacatcta acccgcaata cctaacaggg cggccttgtc
     1081 gccctagtgc tcagattgaa cgcgtcttca cacctatttg gaactactta tgtctgaaaa
     1141 atttgatgtt attgtcatcg ggtcgggccc agcgggctac gttgctgcta ttcgcgccgc
     1201 acaattaggt ttaaaaactg cctgtatcga aaaatggaaa aatagcgacg gcaagggtgt
     1261 taacggcggc acctgtttga atgtgggctg tattccctct aaagcgctgc ttgatagctc
     1321 ctacaaatac cacgaggcgg cccacgccct ggcgggtcac ggtatcgcgg nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccatg atcgcccgca aagaaaccat
     1441 cgtgaagcaa ttgaccggcg gcatcgcagg tttgtttttg gctaacaaag tgaccgccct
     1501 gtacggcacc ggcaagttac tggcgggccg caaggttctg ttcactgact ttgagggcaa
     1561 agagcaaacc ctggaagctg acaatgtgat tctcgcttca ggctctatcc ccgtgaacat
     1621 tcccgttgcc ccagtggata acgatgtgat tgtggattcc actggcgccc tggcatttga
     1681 tcgcgtaccc ggccgcttag gtgtcattgg tgcgggcgtc atcggccttg aactgggctc
     1741 agtttggaat cgtttaggtt ctaaagtggt ttgtttagag gctatggagt cgttccttgc
     1801 cctgatggac caacaaattg ccaaagaaac ccagaaaatc cttaccaaac agggtttgga
     1861 tatccgctta agctcccggg tcaccggcac tcaaattaaa actgtcggtg cggaaaaaga
     1921 agttgaagtt acctacatcg acaaagaagg caaagaagtt aaagaaacct tcgataaatt
     1981 aatcgtctgc gtcggtcgtc gtcccttcac tcaaggcctc ttggcggaag attccggcgt
     2041 taagctggat gagcgcggtt ttatttacgt taacgacctt tgctctacca gcgcccctgg
     2101 cgtgtgggct atcggtgacg tagtgcgtgg ccccatgctg gcccacaaag gctcagaaga
     2161 aggcgtgatg gttgccgagc gtattgctgg tcacaaagcg gtgctcaact acgacattat
     2221 cccaagtgtg gtgtacaccc acccggaagt agccgcagtg ggtaaaacgg aagagcagct
     2281 caaggccgcc ggtgaaaact acaaagtggg cacctttccc ttcgtggcca gtggccgcgc
     2341 cttggccgcc gatgaaaaag acggcttggt taaaattatc gcccacgccg acactgaccg
     2401 cattttgggg gctcacattg tcgggccatc tgctgcggac ctggtgcagc aggttgccat
     2461 tgccatggag tttggttcca gcgcagaaga cttgggcatg atggtgtttg gccatccgac
     2521 cctatctgaa gccgtgcacg aagctgcctt ggcagttaac ggtcacgcga ttcacatcgc
     2581 caaccgcaag aaaaaataag tttggttgcg ctcgcgcagc tagtcgcggc acttttgggt
     2641 cacaaacctg agggtgcctt ttgttcctag ggcattccca cggggcaata aatgtccaac
     2701 gaataggcca caccagcttg ttcactatgt ttaactcgcg ccaatgcgcg caatcaaacg
     2761 gatacatgtc atgaatttgc atgaatatca ggcaaaacag ctgtttgccg aatacggttt
     2821 gcccgtttcc aagggtgtag ctgcggctac tcccgccgag gctgccgccg ctgccgatac
     2881 tattggcggc aatctgtggg tggtgaaagc tcaagttcac gcgggcggcc gcggtaaggc
     2941 cggtggcgtt aaattggtat ccaccaaggc tgaaattgaa gagtttgcca gcaaatggct
     3001 gggcaagaat ctggttacct accaaactga cgcgaagggc cagccggtta gccgtatttt
     3061 ggt
//
LOCUS       DAOQGS010000503         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19021_length_3062_cov_5.378783, whole genome shotgun sequence.
ACCESSION   DAOQGS010000503 DAOQGS010000000
VERSION     DAOQGS010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19021_length_3062_cov_5.378783"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1043
                     /locus_tag="PKZ52_15370"
     CDS             345..1043
                     /locus_tag="PKZ52_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNG61201.1"
                     /translation="MAGLNKVVTSYDEALAGLTDGMTIIAGGFGLCGIPEGLIAQIKK
                     MGTRQLTVVSNNCGVDGFGLGILLENHQIKKMISSYVGENALFEKQLLSGELEVELTP
                     QGTLAEKMRAGGAGIPAFYTATGYGTPIGEGKEVRMFNGRPYILEESIRGDFAIVKGW
                     KADTYGNVIYRHTAMNFNPMAATAGKITVVEVEEIVAPGELPMDHIHTPGIYVDRVIQ
                     GTFEKRIERLTTQA"
     gene            1053..1709
                     /locus_tag="PKZ52_15375"
     CDS             1053..1709
                     /locus_tag="PKZ52_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNG61202.1"
                     /translation="MTLSREQIAQRVARELQDGFYVNLGIGIPTLVANYIPAGMEVML
                     QSENGLLGMGPFPHPNAVDPDMINAGKQTVTAVTGASIFSSAESFAMIRGGHVDLTVL
                     GAFEVDIEGNIASWMIPGKLVKGMGGAMDLVAGADNIIVTMTHASKDGESKLLNRCNL
                     PLTGAGCIKKVVTDLAYIEIHDGKFWLIERAPGVSVDTIVKLTEGPIVVPEVVPEMQF
                     "
     gene            2243..3007
                     /locus_tag="PKZ52_15380"
     CDS             2243..3007
                     /locus_tag="PKZ52_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNG61203.1"
                     /translation="MSIRLMLATLAAAVSVAASANEVKPEGYQKLCGQGETCALPAAN
                     LVAFGGAGAFVYKVQAGTFTCDVHAFGRDPAPNQPKTCSIPPIRPKVTKVLRQGSSSS
                     SSSGLPVVTATTLPAGRYAIVSRRSGKALEINADGQIVQNQLNSSSVQEWDISTVAGG
                     YYAIKSVKTGLTLTLADWQTEEGARISQSAWIDAHSQHWNLSSTGNNSFSLVSRFNGK
                     ALDVVALNTQDQAPVRLWTYWGGDNQQWQFVKVAGN"
BASE COUNT          811 a          806 c          750 g          695 t
ORIGIN      
        1 tcatcgcccc atcaaccagt gttaccgcag taattgggcg ctcagtagcc gggttggtaa
       61 taacctgctc gcgcgctcca gcctgtaaaa caccgccaat caggctgtgc cccaacagca
      121 aagatgggtc ctgtaactgc accataaaag aaatactcgc cgacaaactg atgcagcatc
      181 ataacaagta aacaaccgca ccgatgaagg cccactcctt ggcaaaatat ccttgggcta
      241 aggcgcaaat actgctataa catgcctgct gcaccgcaaa acgtgtaatg gcttaaagcc
      301 cctggctgtc ccaggttttt aagaattctt cagaggaaca cctaatggct ggtttaaaca
      361 aagtagtcac atcctacgac gaggcattgg ccgggctcac cgatggtatg accatcattg
      421 ccggcggctt cggtctgtgt ggaattcctg aggggctgat cgctcagata aaaaaaatgg
      481 gcacacgcca actcacggtg gtttccaata attgcggcgt agatggattt ggcctgggta
      541 tattgttaga aaaccatcaa atcaaaaaaa tgatctcctc ctacgtgggt gaaaatgctc
      601 tatttgaaaa acaactcctc agtggcgaat tagaggtcga gctcacccca cagggtacac
      661 tggcagaaaa aatgcgcgct ggcggcgcgg gcattcccgc cttttacacc gctaccggct
      721 acggcacgcc cataggggaa ggcaaagagg tgcgcatgtt taacggccgc ccttatattt
      781 tggaagaatc cattcgcggc gattttgcca ttgtgaaagg ctggaaagct gacacctacg
      841 gcaatgtgat ctaccgccat accgccatga acttcaaccc catggccgcc accgcaggca
      901 aaatcacggt agtagaggta gaagaaattg tcgccccagg ggagctgccc atggatcaca
      961 tccacacgcc aggcatttat gtggatcggg tgattcaagg cacctttgaa aaacgcatcg
     1021 aacgcctcac cacccaagcc taggagccca ttatgaccct ctcacgcgaa caaatagccc
     1081 agcgggtggc ccgcgaatta caagacggct tctacgtcaa tctcggtatc ggcatcccta
     1141 cactggtggc caactatatt ccggcgggta tggaagtgat gctgcagtcg gaaaacggcc
     1201 tgctgggtat ggggccgttt cctcatccta atgcagtaga cccagacatg attaacgcgg
     1261 gtaaacagac ggtaactgct gtcactggcg cgtctatttt ctcctccgcc gaatcctttg
     1321 caatgattcg cggcggccac gtggatttga ccgttttggg cgctttcgag gtcgatatag
     1381 aaggcaatat tgcctcttgg atgatccctg gcaagctggt taagggcatg ggtggcgcca
     1441 tggatttagt cgccggtgcc gataacatca tcgtcaccat gacccacgcc agtaaagatg
     1501 gcgaatccaa actcctcaac cgctgcaact tgccactcac cggcgccgga tgcatcaaaa
     1561 aagtcgttac cgatttagcc tatatagaaa tccacgacgg taaattttgg ctcatcgagc
     1621 gtgcgccggg cgttagcgtg gataccattg taaaactcac tgaagggccg attgtggtgc
     1681 ctgaggtggt tcctgaaatg cagttctaac ccatctagca attagattac atcaatccac
     1741 ccgccgccaa gctcgcggcg ggtactccac ctcccggcat ttttaaaaac ttagccctat
     1801 agctccaggg cgaaacgccc cctcacaacg tataaaaaca gcgatattgc agcggcctca
     1861 ttaaattggc agatggaatt accaacgacg atctgcgtaa ctaagactaa actttcattt
     1921 tattaggcgg tcagtcgaga gcgcgtttag attattttag aaagggccag gtaaatactc
     1981 ggcgttaagc ctttgcacca acactttgtg cacagcagca aaacaaggtt aaatctgccg
     2041 tttttctcaa gtaccactca ataattgcaa aacctaaaaa tgctttggcg cctgaataga
     2101 attcttttac caaaatgaaa cctaattaac agcattaagg aataaatgaa aaatatcgct
     2161 caattattaa gcaaaattag agactattta acggtacggt tgtatagtgc aggctgtgaa
     2221 tttttctcgc tcggagtgag ttatgtcgat taggcttatg ttggctaccc tggctgcagc
     2281 agtgtcagta gccgcttcag ccaatgaagt gaaacctgag ggctaccaaa aattgtgtgg
     2341 ccaaggagag acctgcgcgc tgccagcggc taatttagtt gccttcggcg gtgcgggcgc
     2401 atttgtttac aaggttcaag cgggtacttt tacctgtgat gtacacgcct tcggtcgcga
     2461 tcctgcgccc aaccagccca aaacctgttc tattccccct atccgtccta aagtcaccaa
     2521 ggttctgcgc caaggctcat ccagcagcag ctcatccggg ctgcccgtgg taaccgccac
     2581 cacattgcca gcagggcgtt acgccatcgt ttcgcgccgc tccggcaagg cgttggagat
     2641 aaacgccgac ggccaaatcg tgcaaaatca gttaaacagt agctctgtgc aagagtggga
     2701 tattagtacg gttgctggcg gatactacgc cattaaatcc gtcaaaaccg gcctaacgct
     2761 taccctcgcc gattggcaaa ctgaggaagg cgcccgtatt agtcagtccg cctggattga
     2821 cgcccacagc caacattgga atttaagctc aaccggcaac aacagcttca gcctggtgtc
     2881 gcgctttaat ggtaaggcat tggacgtagt ggcccttaat acccaagacc aagcgcctgt
     2941 gcgcctatgg acatactggg gtggtgataa tcaacagtgg cagtttgtta aagtcgctgg
     3001 caactaagga acctctgaaa aatgtcggcg gcgaagctct ggctaggcga atttgccaaa
     3061 aa
//
LOCUS       DAOQGS010000504         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19126_length_3051_cov_3.919226, whole genome shotgun sequence.
ACCESSION   DAOQGS010000504 DAOQGS010000000
VERSION     DAOQGS010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19126_length_3051_cov_3.919226"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1443)
                     /gene="bioA"
                     /locus_tag="PKZ52_15385"
     CDS             complement(121..1443)
                     /gene="bioA"
                     /locus_tag="PKZ52_15385"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNG61204.1"
                     /translation="MIDPEYFQRLSADELIRQDRALLWHPYAASPSAQPIYPVARADG
                     CTLTLTDGRQLVDGMASWWAAIHGYNHPALNAALTAQTQRMAHVMFGGLTHAPAVELA
                     HRLVALTPAGLERVFLADSGSVAVEVAIKMAVQYWQGKGKPHKQQFISLRRGYHGDTL
                     GAMSLSDPDTGMHSLFHAIVPQQIFAPAPQSPFAGPMHTQDIEALETLIKEHHNHCAA
                     LVLEPIVQGAGGMRFYHPDYLKAAQELCKRYELLLIADEIATGFGRSGRLFACEHAGI
                     TPDILCLGKALTGGYLTLAATLCTQPVAQGIGQSSAGVFMHGPTFMANPLACSVANRS
                     IELLMDGQWRGQVAAIEAQLNEELAAARAFNCVADVRVLGAIGVIEFTQPLDLNNLQP
                     FLVEQGVWLRPFGKLLYTMPPYIIRPDELSRITQAMVQAARLRENNLL"
     gene            complement(1436..2059)
                     /locus_tag="PKZ52_15390"
     CDS             complement(1436..2059)
                     /locus_tag="PKZ52_15390"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNG61205.1"
                     /translation="MPKDPSRFSKNTLLAVILCGGASSRMGQDKATLCCDGQMLWQQV
                     AKRLAPQVGQLLVSTSARQAALSFAPYPCVVDGQVAAGPLGGISSALASPLVDGFDWV
                     VFSSCDTPLQPTDWVAALAAARAGNRGISYIRYQGQAHYLHALWHRSLLKPLEEFLQA
                     GGRAVHRFYAQVQAQPVDYAPLAAPPIDPFSNLNSPADLARIKPTYD"
     gene            2148..2801
                     /gene="pdxH"
                     /locus_tag="PKZ52_15395"
     CDS             2148..2801
                     /gene="pdxH"
                     /locus_tag="PKZ52_15395"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNG61206.1"
                     /translation="MDLETIRREYLMGGLRRGNLADSPFDQFERWMSDAINSRIPDPT
                     AMCLATVDAQHRPHQRLVLLKKSSPEGLVFFTNLNSAKAQHIAANAQVSLHFPWHMME
                     RQVRVEGVAHKLSVTEALAYFITRPQESQLAAWASPQSQTISSRAMLLQQFHAMKEKF
                     KQGAIPLPDFWGGYRVVPERFEFWQGGANRLHDRFEYLLTTASDGAERTWQINRLAP"
     gene            2798..>3051
                     /locus_tag="PKZ52_15400"
     CDS             2798..>3051
                     /locus_tag="PKZ52_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNG61207.1"
                     /translation="MSQMQFVTFDQPGPASSLYLAACRKPSVNPGEVLIKVAAAGVNR
                     PDLLQRKGLYPAPEGANPILGLEVAGVISAVGTGVRDWRVG"
BASE COUNT          615 a          874 c          937 g          625 t
ORIGIN      
        1 caactggcga gggttacggt gcatttgccc cgccgcaaaa catccgccag ggtaatggct
       61 tcaatttctt cactgccgtg ggcgatgggg attaagactc gttgcatagg ggctcctcgg
      121 ttaaagcagg ttgttctcgc gcaggcgcgc cgcctggacc atggcctggg taatgcggct
      181 gagctcgtcg gggcggatga tatagggcgg catggtatag agcagcttgc cgaaggggcg
      241 cagccaaacg ccctgttcca ctaaaaacgg ctgcaggttg ttaaggtcca gcggttgggt
      301 aaattcaatc acgcctatgg cccccaacac tcgcacatcc gccacgcagt tgaaggcgcg
      361 ggcggcagcg agttcttcat ttaactgcgc ctcaatggcg gccacctggc cccgccactg
      421 cccgtccatt aacagctcaa tactcctgtt ggccaccgag caggcgaggg gattggccat
      481 aaaggtgggg ccgtgcataa acacaccggc tgaactttgg cctatgcctt gggccaccgg
      541 ctgggtgcat agcgttgccg cgagggtgag atagccgcca gtgagggctt tgcccaggca
      601 gagaatatcc ggcgtgatac ctgcgtgttc acaggcgaag aggcggccgc tgcggccaaa
      661 ccctgtggcg atttcatcgg caatcagcag caattcatag cgtttgcaaa gctcttgcgc
      721 ggctttaagg tagtccgggt ggtaaaagcg catgccacct gcgccctgca ctatgggttc
      781 gagcactagg gcggcgcagt ggttgtggtg ctcctttatc aatgtctcca gtgcctcaat
      841 atcctgcgtg tgcattggcc cggcgaaagg gctttggggg gcgggggcaa agatctgttg
      901 gggcactatg gcgtgaaata ggctgtgcat accggtgtcg gggtccgaca ggctcatggc
      961 cccaagggtg tcaccgtgat agccccgccg caggctgata aattgttgtt tgtgcggctt
     1021 acccttgcct tgccaatatt gcacggccat ttttatcgcc acctccaccg ccacgctgcc
     1081 ggagtccgct aagaacaccc gctcaagccc cgccggggta agcgcaacca agcgatgggc
     1141 cagctccacc gccggggcgt gcgtgaggcc gccgaacatt acgtgggcca tacgctgagt
     1201 ttgcgctgtg agcgctgcgt taagggccgg gtggttgtag ccgtgaatgg ccgcccacca
     1261 actggccatg ccatccacta attgcctgcc atcggtgagt gtaagcgtgc agccgtctgc
     1321 ccgcgctact gggtaaattg gctgcgctga ggggctggcg gcgtaggggt gccagagtag
     1381 ggcgcgatcc tggcggatca gttcatcggc gcttaaacgc tggaaatact cagggtcaat
     1441 cataggtggg tttaattcgg gcgagatcag caggggaatt aaggttgcta aatgggtcta
     1501 tgggcggtgc tgccaggggg gcatagtcta ccggttgagc ctgcacctgg gcataaaacc
     1561 tatgcacggc tcggcccccg gcctgtaaaa attcctctaa gggtttaagc aagctgcggt
     1621 gccagagggc gtgcaaataa tgggcttgac cctggtagcg gatatacgag atgcccctgt
     1681 tgccagccct tgcggcagca agggccgcca cccagtcggt gggctgcaaa ggtgtgtcgc
     1741 agctgctaaa gacgacccag tcaaacccat ccacaagcgg gcttgccagt gcagagctga
     1801 ttccgcctaa ggggcctgcc gcaacctgcc catccacaac acaggggtag ggggcgaaac
     1861 tgagcgcagc ttggcgtgcg gacgtactaa ccaaaagctg ccccacctgg ggcgccaaac
     1921 gctttgccac ctgttgccag agcatctggc cgtcgcagca taaggtggct ttatcctggc
     1981 ccatccggct gctggcgccg ccgcagagaa tcaccgccaa aagtgtattt ttgctaaaac
     2041 ggctagggtc ttttggcata cactgtctca tttgcacggg cgctggatga tggacgggcg
     2101 ccgggcggca aagcttaaca aacccctcta gtaagatgtg agatatgttg gatttagaga
     2161 cgattcgccg agaataccta atgggcgggc tgcgccgggg caatcttgcc gatagcccgt
     2221 ttgaccagtt cgagcgctgg atgagcgatg ccattaacag ccgtattccc gaccccactg
     2281 ccatgtgctt agccacggtg gatgcgcagc ataggcccca tcagcgctta gtcctgctga
     2341 aaaaatcctc cccagaaggc ctggtgtttt tcaccaattt aaacagcgcc aaagcccaac
     2401 acatagccgc caatgcccaa gttagcctgc attttccttg gcatatgatg gagcggcagg
     2461 tgcgcgttga gggggtggcc cataagttat cggtcactga agccctcgcc tactttatta
     2521 ctcgccctca ggagagccag ttggccgcct gggcctcgcc ccagagccaa accatcagct
     2581 cccgcgctat gttgctgcag caatttcacg ctatgaaaga aaaattcaaa cagggcgcta
     2641 ttccgctgcc ggatttttgg ggcggctacc gggtggtgcc tgagcgcttt gaattctggc
     2701 agggcggcgc caaccgcttg cacgatcggt ttgaatatct gctcaccact gccagtgatg
     2761 gggcagagcg cacttggcag atcaacaggc tggccccatg agccagatgc agtttgtcac
     2821 cttcgatcaa cccggcccag cctcaagcct gtatctcgcc gcctgccgta aaccctccgt
     2881 taaccccggc gaggtgttga ttaaggtggc cgccgccgga gtgaaccggc cggatctttt
     2941 acagcgcaaa ggtctctacc cggccccgga aggcgccaat cccattctgg gtttagaagt
     3001 ggccggcgtc atcagcgctg taggcaccgg tgtgcgggac tggcgggtgg g
//
LOCUS       DAOQGS010000505         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19160_length_3048_cov_3.552289, whole genome shotgun sequence.
ACCESSION   DAOQGS010000505 DAOQGS010000000
VERSION     DAOQGS010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19160_length_3048_cov_3.552289"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /gene="ilvB"
                     /locus_tag="PKZ52_15405"
     CDS             complement(<1..1453)
                     /gene="ilvB"
                     /locus_tag="PKZ52_15405"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNG61208.1"
                     /translation="MELLSGGDMLMRALADEGVECIFGYPGGAALHIYDALFRQKQIQ
                     HILVRHEQAATHAADAYSRATGRTGVVLVTSGPGATNAITGIATAYMDSIPMVVISGQ
                     VPSDKIGEDAFQETDMVGISRPIVKHSFLVKDARDIPTIIKKAFYIASTGRPGPVVVD
                     IPKDITSPATTFEYHFPDKVKLRSYTPAQRGHAGQIKKALQLLLQAKRPIIYAGGGVI
                     QGNASAQLIELARRLNAPVTNTLMGLGAFPGTDKQFLGMLGMHGTVEANYAMHHSDVI
                     LAVGARFDDRVTNTPSKFCPSAKIIHIDIDPASISKTVAADIPIVGPVDAVLNEMLDL
                     HKHAEEKQDQEALALWWQQINEWRERYGLLVKPRYTLGDMIKPQEVISMLWEVTKGDA
                     YITSDVGQHQMFSALYYPFDKPRRWINSGGLGTMGFGLPAAMGVQYAFRDATVACVTG
                     EGSIQMCIQELSTCTQYHLPVKIICLNNQALGMV"
     gene            complement(1887..>3048)
                     /locus_tag="PKZ52_15410"
     CDS             complement(1887..>3048)
                     /locus_tag="PKZ52_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61209.1"
                     /translation="RAGNLARMSELQYGIIPDLTKQLDLASHAEMVDMKLLRNRVTDE
                     EIAEVVSKWTGIPVSKMLEGEREKLIRMEATLHERVVGQNEAVTSVANAVRRARAGLS
                     DPNRPSGSFLFLGPTGVGKTELCKSLADFLFDSQDAMVRIDMSEFMEKHSVARLIGAP
                     PGYVGYEEGGYLTEAVRRRPYAVVLLDEVEKAHPDVFNILLQVLEDGRLTDGHGRTVD
                     FRNTVVVMTSNLGSDLIQELANNKSFDMLDFGGGESAEGNEHRYNAMKEAVMGVVSNH
                     FRPEFINRIDEVVVFHPLERKQIHGIARIQLQGLHQRLAERDLAIELSEEVIDKLAEV
                     GFDPVYGARPLKRAIQHMIENPLAHDILSGKFMPGTTIKTQLNGDKIRFFSE"
BASE COUNT          712 a          807 c          795 g          734 t
ORIGIN      
        1 tcaccatgcc caaggcttgg ttattcaagc agataatttt taccggcagg tgatattggg
       61 tgcaggtgga taattcctga atacacattt gaatactgcc ctcaccggta acgcaggcga
      121 cggtggcatc gcggaaggcg tattgcaccc ccatggcggc aggtaagcca aaccccatgg
      181 tgccaaggcc cccggaatta atccaacggc ggggtttatc aaacgggtaa taaagggcgg
      241 aaaacatctg gtgctggccc acatcggagg tgatataggc atcgcctttg gttacttccc
      301 acagcatgga aataacttcc tggggtttga tcatatcgcc cagggtatag cgcggtttca
      361 ccaacaggcc atagcgctcc cgccattcat taatttgctg ccaccacagg gcaagggctt
      421 cttgatcctg tttttcctca gcgtgtttgt gcaagtccaa catttcattc aaaaccgcat
      481 ccacaggccc cacgatggga atatccgctg ctacggtttt tgagatagag gcagggtcta
      541 tatcaatgtg aataattttg gcggatgggc aaaatttact gggggtattg gtgacccggt
      601 catcaaaacg cgcgccgacg gccaaaatca catcgctgtg gtgcatggcg tagttggctt
      661 ctacggtgcc gtgcataccc aacatgccga gaaactgctt gtcagtgccg ggaaaggcgc
      721 ctaagcccat caaggtgttg gtcacaggtg cattgagcct gcgggccagt tcaattagct
      781 gagcgctggc attgccctga ataacaccgc cgccagcata aatgattggc cgcttagcct
      841 gtaacaacag ctggagggct tttttaattt gccctgcgtg gccccgttgc gcaggtgtat
      901 aggagcgcag tttcaccttg tcggggaagt gatattcaaa ggtggtggcg gggctggtga
      961 tgtctttagg aatatccacc accaccgggc ctgggcggcc ggtgctggca atataaaagg
     1021 cttttttgat aatggtggga atatcccgcg cgtccttcac caaaaagctg tgtttaacga
     1081 tgggcctgga gattcccacc atgtcggttt cttggaaggc gtcttcaccg attttatcgg
     1141 agggcacctg acctgagatc accaccatag ggatagaatc catatatgca gtggcgatgc
     1201 cggtaatggc attggtggcg ccggggcctg aggtcaccag caccacgccg gtacggccag
     1261 tggcgcggga gtaggcatcc gccgcgtggg tggccgcctg ttcgtggcgc accagaatat
     1321 gttggatctg cttttggcga aataaagcat cataaatatg taaagctgcc ccacctgggt
     1381 agccaaaaat gcattcaacg ccttcgtctg ccaaagcgcg catcagcatg tcgccgccgg
     1441 aaagtaattc caccgtcatg ttcctcatct gtgataaaaa aatactgccc caggccaggt
     1501 tagctgacat tgatgacaaa tcgtggggcg gtgcgatgtg gtagctctgc cggtacctgt
     1561 gcttttgggg atatacgcag tagctttggc tttggggtta caaagctcgc ttccgatgct
     1621 tgggggtaac agggttgatg tttcccctcg taatgcgacc tgggttgtgc ggtaacatgg
     1681 gccagcgcgg atgcgacctg acattgtgct aactacatgg gaaaagtcaa ccgcttaaaa
     1741 gcctaggagg gggcttaggt actgattttt atagacttgc gtaagcgact gagcctcatc
     1801 ggcacccgcc cactatcctt tttgaccggg cataaaaaag ccaagcggat cttcgcttgg
     1861 cttgtaggct gggattagcc ccagggtcac tcactaaaaa agcgaatttt atcgccgttt
     1921 aattgagttt tgatggtggt tcccggcata aatttacccg acaaaatatc atgggccaga
     1981 gggttttcga tcatatgttg aatggcacgc tttaatggcc gcgcaccata aacagggtca
     2041 aaacccacct ccgccaattt atcgattacc tcctccgata gctcaatggc caaatcccgc
     2101 tccgccaggc gctggtgcaa gccctgtaat tgaatgcggg cgatgccgtg gatttgtttg
     2161 cgctccaagg gatgaaatac cactacctcg tcgatacggt tgataaattc cggccggaaa
     2221 tgattgctca ccacccccat caccgcctct ttcatggcgt tatagcgatg ctcattgccc
     2281 tcagcagact cgccgccgcc aaaatccaac atatcaaagg acttgttatt ggccagctcc
     2341 tgaattaaat ccgaacctag gttcgacgtc atcaccacca cggtattgcg aaaatccacc
     2401 gtgcggccgt ggccatcggt taggcggcca tcttctaaca cttgcaagag aatgttgaaa
     2461 acatccggat gggctttttc cacttcgtct aataacacca ccgcataggg cctgcggcgc
     2521 acggcttcgg ttaaataacc gccctcttca taacccacat aaccgggggg cgcgccaatt
     2581 aagcgggcta ccgagtgttt ctccataaat tccgacatat caatgcgcac catggcgtcc
     2641 tgggaatcaa acagaaaatc ggccagggat ttacacaatt cggttttacc cacgccggtg
     2701 gggcctaaaa ataaaaagct gccgctgggg cggttcgggt cgcttaaacc cgcccgcgcc
     2761 ctgcgcacgg cattggccac ggaagtaacc gcttcatttt gccccactac ccgctcgtgc
     2821 aaagtagctt ccatgcgaat gagtttttcc cgttcgcctt cgagcatttt actgaccgga
     2881 atgccggtcc attttgatac tacctcggca atttcttcat ctgtcacgcg gttgcgcagt
     2941 aatttcatat ccaccatttc agcatggctg gccaaatcta actgcttggt taaatcgggg
     3001 ataatgccgt attgcaattc agacatacgc gccaaattac ccgcccgg
//
LOCUS       DAOQGS010000506         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19223_length_3041_cov_4.356664, whole genome shotgun sequence.
ACCESSION   DAOQGS010000506 DAOQGS010000000
VERSION     DAOQGS010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19223_length_3041_cov_4.356664"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..995)
                     /gene="rsmI"
                     /locus_tag="PKZ52_15415"
     CDS             complement(144..995)
                     /gene="rsmI"
                     /locus_tag="PKZ52_15415"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNG61210.1"
                     /translation="MSRGLTPVGELFIVATPIGNLADMVPRAVEILQNVDFIAAEDTR
                     HSGKLMQHFQINTPLVAYHDYSDETRVKQLLARVQAGECMALISDAGTPLISDPGYRL
                     VHWARQAGIRVTPVPGSCALIAALCASGLPSDRFCFEGFLAAKSSARRGQLEALKNEP
                     RTLVFYESPHRIEECLADLVAIFGSERTAVMARELTKTFETFLTGNLSEIAEQVAGDA
                     NQRKGEIVLMVQGFSAPVDAEALDAEAIRIMEILLSELPVKQAASLTARITGEKKNKL
                     YQWALER"
     gene            1034..2887
                     /locus_tag="PKZ52_15420"
     CDS             1034..2887
                     /locus_tag="PKZ52_15420"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNG61211.1"
                     /translation="MSVFSRWAAISLVPFLLVSCASTPEQPNKPVSQTQSLQQQVQKW
                     LQQAEKAQGPERNALLLQAADGYVRLHNLEQAQAVIDAVPAAELGGESYVKRALIYSL
                     IADRRGNYAAAKEAITGRNVLAQLNSLSGDTAIAARKQRAKLFNESGDYTKAAKERLM
                     LAGLLAGKNLEDQTANNTALWQLLLEWPEAQLATSSNQAKDLNAKAWYELAHLYKSQQ
                     YSTQGQLQALNRWIKRYPEHPASKQLPKELLDLQQQLADLPKQIALLMPQSGKLEAAA
                     SAIVDGLMAAYYQQQRNGALVPSLRFYNTEGARTLDLYQKAVQEGADIVIGPLEKERL
                     AELSQQPSLPVPVLGLNTLEGAGNRQVFQIGLSVEDEAKQIAAQALREGLRKALVLLP
                     ANALGDRNLATFANTFRQQGGAVYEYRYGANTDFGELARRALQIDQSEARVKSLQQMI
                     GPVEATPIPRNDIDMIFAVVQPEQARQLKPNLNFYYAANLPLYTTSSVFSGENSAQNA
                     DLTQTRFLTLPWYFSQSEEKTALEAASDKNPNFARLYALGVDVFNLAPRLKQAQKSAL
                     PAYYGSTGTLSLNNNNQFERLQTWATFDATGNVQLLGNDSSDTRNDSNQNQ"
BASE COUNT          820 a          899 c          720 g          602 t
ORIGIN      
        1 accgatcggc aagtaacttt tacacaagaa aataaaaaat acttttacct ttacacccgc
       61 ttttttgcag aatagtttgc aaaaagaaag acgctctaaa cacggttttt tgaaggccga
      121 gcgcatagat ctaaaaaaca cccttagcgc tccagcgccc attgatacaa tttatttttt
      181 ttctctccag taatgcgcgc cgttaaactc gccgcctgtt ttaccggcaa ttcactcaac
      241 agaatttcca taatacggat ggcttcagca tccaaggctt cggcatctac cggcgcacta
      301 aagccctgca ccatcagcac aatttccccc ttgcgctgat tggcgtcccc ggccacttgc
      361 tcggcaattt cacttaaatt gccggtcaaa aaggtttcaa aggttttggt cagttcccgg
      421 gccattaccg ccgtgcgctc agagccaaaa atcgccacta aatccgccaa acactcttca
      481 atacggtggg gagattcata aaacactagg gtgcgcggct cgttttttaa ggcttctaat
      541 tggcctcgcc gggcgcttga tttggccgct aaaaaaccct caaaacaaaa ccgatccgac
      601 ggcaaaccgc tggcgcacaa agcggcaatt aaggcgcagg aaccgggcac cggcgtcacc
      661 cgaatgcccg cctggcgggc ccaatgcacc aggcgataac ccgggtcgga aatcagcggc
      721 gtgcccgcat cgctaatcag cgccatacac tcgccagcct gtacccgcgc cagcagttgt
      781 ttaacccgag tttcatcgct gtaatcgtgg taggccacta aaggcgtatt gatttgaaaa
      841 tgctgcatga gtttgccgct gtggcgggtg tcctcagcag caataaaatc aacgttctgg
      901 agaatctcca cggcgcgggg taccatgtcg gccaaattgc cgatgggtgt ggccacaata
      961 aaaagttcac caactggggt taaacctcgg ctcatatcaa tgcctactgc ataattgttg
     1021 aaaaggtata ttcatgtcgg tgttttcccg ctgggctgcc atcagccttg tgccgttttt
     1081 gttggtaagc tgcgcctcca ccccggagca accgaataaa cccgtaagcc aaactcaaag
     1141 cctgcaacaa caggtgcaaa agtggctgca acaagcggaa aaagcccagg ggcccgagcg
     1201 caatgccctc ctactgcaag cggcggacgg ctatgtgcgg ctgcataatt tagagcaggc
     1261 ccaagcggtt atcgacgcgg tgcccgccgc cgagctgggg ggggaatcct acgtaaaacg
     1321 cgccctgatt tacagtttaa tcgccgaccg gcgcggtaat tacgccgccg ccaaagaagc
     1381 catcaccggc cgcaatgtgc tggcacagct caattcttta agcggcgaca ccgccatcgc
     1441 cgcccgcaaa cagcgggcaa aactctttaa cgaaagcggt gattacacca aggccgccaa
     1501 agagcgcctt atgttggcag gccttttggc cggtaaaaat ttagaagatc aaaccgccaa
     1561 taacaccgcc ctctggcaat tattattgga gtggcccgag gcccaactcg ccaccagcag
     1621 caaccaagct aaagacctca acgccaaagc ctggtacgaa ttagctcacc tctacaagag
     1681 ccagcagtac tccacccaag gccaattaca ggcgcttaac cgctggataa aacgctaccc
     1741 agaacacccc gccagcaaac aactgcccaa agaattgctg gacctacagc aacaactggc
     1801 ggacctaccc aaacaaatcg ccctcttaat gccgcaaagc ggcaagctcg aagccgccgc
     1861 cagcgccata gtggatggcc tgatggccgc ctattaccaa cagcagcgca acggcgccct
     1921 ggtgccaagc ctgaggtttt ataacaccga aggcgcccgt acactggatc tataccaaaa
     1981 agccgtacag gaaggtgccg acatagttat aggcccattg gaaaaagaac gcctggcaga
     2041 attaagccaa cagccttcgc tgccggtgcc cgttctgggc cttaataccc tggaaggagc
     2101 aggcaaccgt caggtgtttc aaatcggctt gagtgtagag gacgaagcca aacaaatcgc
     2161 cgcccaagcc ctgcgcgaag gcctgcgcaa agcgctggta ctgctgccgg ccaacgcttt
     2221 aggggatcgc aacctggcca cctttgccaa caccttccgc cagcagggcg gcgccgtgta
     2281 cgaataccgc tacggcgcca acaccgattt tggtgaactc gcccgccgcg ccctgcaaat
     2341 tgatcaaagc gaagcccgcg ttaaatccct gcagcaaatg atcggcccgg tggaggcaac
     2401 gcctattccc cgcaacgaca tagatatgat tttcgccgta gtacaaccag aacaagcgcg
     2461 gcaattaaaa cctaatttaa atttttatta cgccgccaat ttaccgctct ataccaccag
     2521 cagcgtattt agcggcgaaa atagcgcgca aaatgccgac ttaacccaaa cgcgtttttt
     2581 aaccctgccc tggtatttca gccagagtga agaaaaaacc gcgctggaag cagccagcga
     2641 taaaaacccc aatttcgccc gcttatacgc cttgggtgtg gacgtattta atttagcgcc
     2701 gcgtttaaaa caggcacaaa aaagcgccct gcccgcctac tacggctcca ccggcacatt
     2761 gagcctgaat aacaataatc aatttgaacg cctgcaaacc tgggccacct ttgatgcaac
     2821 cggaaatgtg cagttgcttg gcaatgacag cagcgatacg cgcaatgaca gcaaccaaaa
     2881 tcaataaacc cttaaatacc agtaaaaata caggcgccgc tgccgaagcc ctggcgcgca
     2941 cttatttaca ggagcagggt tggcacgtgg tggcccataa ttttcgcagc agctacggcg
     3001 agatcgacct aattattcaa cagggccaat ggctcgcctt t
//
LOCUS       DAOQGS010000507         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19227_length_3041_cov_3.057602, whole genome shotgun sequence.
ACCESSION   DAOQGS010000507 DAOQGS010000000
VERSION     DAOQGS010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19227_length_3041_cov_3.057602"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKZ52_15425"
     CDS             <1..366
                     /locus_tag="PKZ52_15425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG61212.1"
                     /translation="GNSAGNSIEYLKTCQQNYPVIYLDPMFPERQKSAAVKKEMQLFH
                     ALIGKDDDSAELFAQALPKAWCRLVVKRPRLAPQLTDTKVNLVLEGKSCRFDIYTQRK
                     ITAESLAECEAGKSEERPA"
     gene            complement(417..1886)
                     /locus_tag="PKZ52_15430"
     CDS             complement(417..1886)
                     /locus_tag="PKZ52_15430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HNG61213.1"
                     /translation="MQRLSTAYLPAIKSAKPNYNRANLKPGIVHLGIGAFHRAHQAVY
                     TDTVMNTMGGDWGIIGCSLRSDAVKQQLQPQDGLYTVLERGSENTPRIIGSVLEVLVA
                     PENPSAIIDAIALPSVKLVSLTITEKGYCHLPASGQLNLSHPDIQQDIAHINAPKSAP
                     GFIVAGLYKRFLNKLEPVSILSCDNLPHNGAVTRAVVIGLAEQIDPALAAWIKNTISF
                     PATMVDRIVPATTAADIESFSEQYGYVDAGLVVAEPFSQWVIEDDFCNLRPAWERAGA
                     LIVKDVAAYETMKLRLLNGSHSLLAYAGFLAGFATVYETMQDAQLLKLTEYFMRAAAA
                     SLQAPEHFDLAAYQAQLIQRFQNPGLQHRTAQIAMDGSQKIPQRWLNSLRDTQQIGAN
                     TDIFAFALATWLRYLQGVNENGASIPISDPLAADLSALSATFSGDYQALVGRFFAFEA
                     VFGQDLQGSTALIECTAYWLAIINKRGIKSAIEHFLAQR"
     gene            complement(1924..2826)
                     /locus_tag="PKZ52_15435"
                     /pseudo
     CDS             complement(1924..2826)
                     /locus_tag="PKZ52_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
BASE COUNT          776 a          734 c          774 g          757 t
ORIGIN      
        1 ggtaattctg ctggcaatag tatcgagtat ttaaaaacct gccagcagaa ttaccccgtg
       61 atttaccttg accctatgtt ccccgagcgg caaaaaagcg cggcggttaa aaaagaaatg
      121 cagttatttc acgccttgat cggcaaagat gatgacagcg cagaattatt cgcccaggcc
      181 ctgcccaaag cctggtgccg cctggtggta aaacgccccc gcctggcgcc gcagttaacc
      241 gacactaaag tgaatttggt tttagagggt aaatcttgcc ggtttgatat ttatacccag
      301 cgaaaaataa cggctgagag tttggctgag tgtgaagccg gcaagagtga agaaagaccc
      361 gcttaaggct attgcctggc ggcgcagcta gccaaaacca gacaataaaa accggtttac
      421 cgctgagcga gaaaatgctc aattgctgat ttaatgccgc gcttattgat aattgccagc
      481 cagtacgccg tgcattctat tagcgctgtg ctgccttgca gatcctgacc aaacacagct
      541 tcaaacgcaa aaaaccgccc gaccaatgct tgataatccc cgctaaatgt tgcactcagc
      601 gcgcttaaat cggcagcgag cgggtcagaa atgggaatac tcgcgccgtt ttcattcacc
      661 ccctgtaaat aacgcagcca agttgctaac gcaaacgcaa aaatatccgt attggcccct
      721 atttgttgtg tatcgcgcag gctgttcaac caacgctggg gaattttttg cgagccgtcc
      781 atggcaattt gcgctgtgcg atgctgcaag cctgggtttt gaaagcgctg aattaattgt
      841 gcttggtagg ctgccaaatc aaaatgctca ggcgcctgta aactagcggc ggctgcgcgc
      901 ataaaatatt cggtgagttt gagcaactgg gcatcctgca tggtttcata aacagttgcg
      961 aagcctgcta aaaaacctgc gtaggccagt aggctgtggc tgccatttaa taaccgcaat
     1021 ttcatagttt cataggcggc cacatctttg acgattaaag caccggccct ttcccaagcg
     1081 ggccgcaggt tacaaaaatc atcttcaata acccattggc taaaaggctc tgccaccact
     1141 aaacctgcat ccacataacc gtattgttcg ctaaaggatt caatatcagc cgctgtggta
     1201 gcaggcacaa tgcgatccac catggtagcg ggaaaactaa tggtgttttt tatccaagcg
     1261 gcgagggctg ggtctatctg ctcggctaat ccgattacca ccgcccgtgt tactgcgccg
     1321 ttatggggta aattatcgca gctgagaata ctgacgggtt ctaatttatt caggaagcgt
     1381 ttatataaac ccgccacaat aaaacccggt gcggatttag gcgcgttgat atgcgctata
     1441 tcctgttgta tatccgggtg ggataaattt aattggccgc tggcgggtag gtggcaataa
     1501 cctttttctg tgatggttag cgagacgagt tttacactcg gcagcgcaat tgcgtcgatg
     1561 attgcactag ggttttccgg cgccactaat acttcgagca cagaacctat aatgcgcggc
     1621 gtattttcac tgccacgctc taataccgta taaaggccat cctggggctg taattgttgt
     1681 ttgaccgcat ccgagcgcaa actgcagcca ataattcccc agtcgccacc catagtattc
     1741 ataacagtat cggtgtacac cgcttgatgg gcgcggtgaa atgcaccaat acccagatgc
     1801 actatgccgg gttttaaatt ggctcggttg tagttgggtt tggcgctttt aattgcgggt
     1861 aaataagcgg ttgacagtcg ttgcatggat gtctcgatat taataatttt tattaacagc
     1921 ctattaaagc ttataggctt tcttaaccag gttataggtt aaatccaccg ctaattcctg
     1981 cgcctcgtct tcatgcaagc gatgctctgc cactaactgc gccaagaata cacagtccat
     2041 gcggcgggct acatcgtggc gggcggggat ggataaaaat gcgcgggtgt catcattaaa
     2101 ccccaccgta ttataaaaac cggcggtttc ggtggtttgt tcgcggaatc tgcgcatacc
     2161 ttctgggctg tcgtggaacc accaggcggg gccgagtttt aagcaggggt agtggcccgc
     2221 caggggtgca agctctcggc tatacacggt ttcatcgagg gtaaataaaa taatcgttaa
     2281 atttttctcg ttgccaaatt tatccagcag cggttttagg gcctgcacat aagaggttgg
     2341 cgtgggaata tcggcaccct tatcgcggcc aaagcggcta aagagactgg cattgtgatt
     2401 gcggaacgag ccggggtgta attgcatcac caagccatct tctaaactca tggcggccat
     2461 ttgagttaac atttgggcgc gaaataattc ggcctcttgc gcattgcatt tacctgcaat
     2521 ggcgcgggca aataaggctt ctgtttcgct gcgggttaaa ttcgctgttg ctgcagtcgc
     2581 gtggccgtga tccgtggcgg tggcacccag cgcacggaaa tagtcccgtc tttgctgaag
     2641 ggcatttaaa tagccagtaa aactaaacgt gtcctcccgc gttaattccg ctaagtgttt
     2701 aaggttaggc aaaaagcctt caaaatccgg atccattacg ggatcggggc ggaacgaggt
     2761 aattacccgg cctcgccagc cactcgcgcg aattttttga tggtggggca aatcatccag
     2821 cggtgctgca ccagcggctt cttacaccgc tcctccgttc aattatacag agcccgccgt
     2881 aacaggaggc aagttaaaag gagtggttga actcggggca acaggcttca actcttttat
     2941 cgttcgcatg gacgcacaga aaaactggaa actggaaaaa gcagagtatg gagcaagtct
     3001 ggtgtatgaa aagctcgcca cagatgatga cattaaaagt g
//
LOCUS       DAOQGS010000508         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19347_length_3029_cov_5.039677, whole genome shotgun sequence.
ACCESSION   DAOQGS010000508 DAOQGS010000000
VERSION     DAOQGS010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19347_length_3029_cov_5.039677"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3029)
                     /locus_tag="PKZ52_15440"
     CDS             complement(<1..>3029)
                     /locus_tag="PKZ52_15440"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG61214.1"
                     /translation="RPMRGLVSRQLLGVVLSLDIALQPLWALAADGTVASTAANAATQ
                     VTQAANGVEVIQIADPNAAGVSHNLFQDYQVGPQGQVLNNSTDDVRSKLAGTIEGNSQ
                     LQHGPASIILNEVTGANPSSLLGVTEVAGQAASLVIANPNGITCSGCGFLNTPRVTLG
                     TGRPLWQEGHLTGLTVDKGILEIGQDGLLGGNLEKLELLAKQIVLYGQVVSPDLTLAA
                     GSLKFNYADGRIEQRGVLPGSVLPYLAIDASALGAMYANKIALVATDNGVGVNVEGPI
                     AAYTGSIHIDANGEVRASDVQAQQNLSIATQGKVTLDKALANSVDINTTDAVQLNSTL
                     AAQEQLSVQAKSIQNLGTVSAGYRPDGSLNNKANLSLIAEGKADNQGQWLSAGSLAIE
                     AADLTNRAGAMISGREGLSLIAGAGSNRGELNSLGALTLTIDGLWDNQVGRVQTAGQL
                     TLNADAWNNSQGHTQAADLSTQIESSLNNTQGEVVTTQGNLIVDAAELINQQGVLQSQ
                     NAVTVTATNLDNTQGSVTVKGGETSKLTLDGTLTNGGGTVAIASNKTNLNAKILDQSA
                     KSSGQAGGALEFTGSGAVDITAEQINNTGGKIAGSGTSLTLKAQELNNQNGTLRSGQA
                     PTKPQETSSASTTPAATSSQLTLALSGDLNNQQGRVLSDNLSLSAASLDNRAGYIDAQ
                     STQGTTQINISGDIRNQTGTVFSKQNTLSLQAAQWQNAQGQIGHLGSGVFTLNAQQMD
                     NTAGKVQTFGGAQWQLTGWNNTQGVVSIRGDGDANSAITLSEDWINEQGRLIVGGQDF
                     TFTARSVHNNGLDETQGIWHSGSGAWALTAETLTNTAGKLGSAGDFKLTLNQLENAGE
                     LQAHQLTLKANSATNGGGITLSSTAANSLDAQVQGDWLNQNGKITLAGGGLITVNRWD
                     NTNGQLLAAGTINLTAQGGDTNQASFINKNGWLQADGGLTLTTASTLDNRQGKLLAGG
                     DTLLTSQAGINNTSGTIDLTPAVTAETTKATAPSLTI"
BASE COUNT          550 a          831 c          868 g          780 t
ORIGIN      
        1 gatggttaaa ctaggcgcag tggctttagt ggtttcagcg gttactgccg gggtgaggtc
       61 aatagtaccg ctggtgttat tgatgcccgc ctggctggta agcagggtat ccccgcccgc
      121 gagcaatttg ccctggcggt tatccagggt acttgccgta gtaagcgtta aaccaccatc
      181 ggcttgcagc caaccgtttt tgttgatgaa ggaggcctgg ttggtatcgc cgccctgagc
      241 ggtgagattg atggtaccgg ccgcgaggag ctggccattt gtattgtccc agcggttgac
      301 ggtgattaaa ccgcctccag caagggtgat tttgccgttc tggtttaacc aatcgccttg
      361 cacctgggca tccaggctgt tagcggcagt gctggaaagg gttataccgc cgccattggt
      421 ggcgctgttg gctttgaggg tcagttgatg cgcttgcagt tcaccagcgt tttccaactg
      481 gttcagcgtc agtttaaagt ccccagcact gcccagtttg cctgctgtgt tggtgagcgt
      541 ttctgccgta agcgcccacg ccccgctgcc gctgtgccag atgccctggg tttcatccag
      601 gccgttgttg tgcacagagc gtgcggtgaa ggtgaaatct tgcccgccga ctattaggcg
      661 cccttgctcg tttatccagt cctcactcag ggtgatcgcg ctgttggcat caccatcgcc
      721 gcgaatactc acaacacctt gggtattgtt ccagcccgtt agctgccact gggcgccgcc
      781 aaaggtttgc accttgcctg cagtgttatc catctgctgg gcattgaggg taaatacacc
      841 actgcccaag tggccaattt ggccctgggc gttttgccac tgggcggcct gtaggctgag
      901 ggtgttttgt ttactgaaga ccgtaccggt ttggtttcga atatctccgc tgatgttaat
      961 ttgcgttgtt ccctgggtgc tttgtgcatc gatataacct gcgcgattat ccaggcttgc
     1021 cgcgctcagg cttaggttgt cgctaagcac gcggccctgc tggttattta aatcaccgct
     1081 taatgccaag gtcagctggc ttgaggtagc tgcgggcgtg gtcgaggctg atgatgtctc
     1141 ctgcggttta gtgggcgctt ggccgctgcg caaggtgcca ttttggttgt tgagttcttg
     1201 ggctttaagt gtcagggatg tgccggaacc agcaatcttg ccgcccgtgt tgttgatttg
     1261 ctccgctgta atgtccacag cgccactacc ggtaaactcc aaggcgccac cggcttgccc
     1321 ggaagattta gccgattgat ccagaatttt tgcatttaaa ttggttttgt tactggcgat
     1381 agcaactgtg ccgcccccgt tggtaagcgt gccatcaagg gtgagtttgc tggtttcccc
     1441 gcccttgacc gtcacgctgc cctgagtgtt gtccagattg gtggccgtta ccgtaacggc
     1501 gttctggctt tgcagcacgc cctgttggtt gatcagttca gccgcgtcca caatcaggtt
     1561 gccctgagta gtaaccactt ccccctgagt gttattgagg ctggactcaa tctgtgtgct
     1621 taggtccgca gcctgggtat ggccttgact gttgttccaa gcatcggcgt tcagagttaa
     1681 ctgcccggcg gtttgcaccc gcccaacttg gttatcccac aggccgtcga tggtaagggt
     1741 tagagcaccc aagctattga gttcgccgcg attactaccg gcccctgcga taaggcttaa
     1801 cccctcgcgg ccgctgatca tggcaccggc gcggttggtt agatcagctg cctcgatagc
     1861 cagcgaacct gcactgagcc actggccttg gttatccgcc ttgccttcgg caatcaggct
     1921 gagattggcc ttgttattga gggagccatc cggccggtaa cccgccgaga cggtacccag
     1981 gttttggatg gacttagctt gaacgctcag ctgttcttgg gccgctaggg tgctattgag
     2041 ctgcaccgca tcggttgtgt ttatatcgac gctattggcc agggccttat ccagggtcac
     2101 cttgccttgc gtcgcaatgc tgaggttttg ctgggcttgc acgtcgcttg cccgcacctc
     2161 cccattggca tcgatgtgaa tagaaccggt ataggccgct atggggcctt ccacattcac
     2221 acccacgcca ttatccgtgg ccaccaaggc aattttgttg gcgtacatgg cgccaagggc
     2281 cgaggcgtct atggccaggt acggcaatac tgaacctggc aatacaccgc gctgctcaat
     2341 gcggccgtcg gcgtaattaa acttgaggct gcccgctgcc agggtgagat cggggctgac
     2401 cacctggcca tagagcacaa tctgtttcgc caataactcc agtttctcta agttgccgcc
     2461 gagtaaaccg tcctgaccga tttctaaaat acccttatca acggttaaac cggttaagtg
     2521 cccttcttgc cagagcgggc ggccggtgcc caatgtcacc cgtggagtat ttaaaaatcc
     2581 gcagccggaa cacgtaatgc cgttagggtt ggcaatcacc aaactcgccg cttgacccgc
     2641 tacttccgta actcctaaaa gtgaggacgg atttgcgccg gtaacttcat taagaatgat
     2701 actggcgggg ccatgctgca gctggctgtt gccttcaatg gtgcccgcta attttgagcg
     2761 cacgtcatct gtgctgttgt taagcacctg gccctgcggg ccgacttggt agtcttggaa
     2821 taggttgtgg ctgacacccg ccgcattggg gtcggcaatc tggatcacct caacgccgtt
     2881 ggcggcttgg gtaacttgag tagcggcatt ggctgcggtg ctggctacag taccgtcggc
     2941 cgccaaggcc cacaggggtt gtaaggctat atcaaggctt aaaaccacac ccaacaattg
     3001 tcggctaact aatcccctca tgggacgga
//
LOCUS       DAOQGS010000509         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19413_length_3024_cov_4.568542, whole genome shotgun sequence.
ACCESSION   DAOQGS010000509 DAOQGS010000000
VERSION     DAOQGS010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19413_length_3024_cov_4.568542"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..2809)
                     /gene="polA"
                     /locus_tag="PKZ52_15445"
     CDS             complement(65..2809)
                     /gene="polA"
                     /locus_tag="PKZ52_15445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNG61215.1"
                     /translation="MSEPAPLVLVDGSSYLYRAFHAAPPLTNAAGEPTGAVKLVLNMV
                     RRLLKDYPSSPIAMVFDAKGKTFRDDLFEHYKAHRPPMPDDLRAQIEPIHQAITAMGL
                     PLIVIEGVEADDVIGTLACQGAAQGQKVIISTGDKDMAQLVNPWVSLHNTMTETRMDE
                     AGVVEKFGLPPELIIDFLALMGDKVDNIPGVPGVGEKTAQALLSQLGNLDAVYQRLDE
                     VAGLPIRGAKSLAEKLREHEAMARLSYQLATIKTDCELPFSLADLHAKPADNATLKAL
                     YERLEFKSWINELNAAPSANAPVEENPGEEQVQTAEPSINYQCVFTLEELNAWITKLK
                     QAEYFAFDTETTSLNYMEAKIVGVSFACTPGEAAYVPFGHDYLGAPAQLTQEQVLGAL
                     KPLLENPQIKKLGQHSKYDVHVLANAGIHMQGIAFDTLLEGFVVNSTTRQDMDSLAEI
                     YLQRQTIHFEDIAGKGAKALTFNQIAIEKAAPYAAEDADITLRLHQTLYPKIAQDAGL
                     LNVYNQIEIPLLPVLQRIERTGALIDAKLLQQQSLQLAAQLDQLQKEAFELAGEEFNL
                     SSPKQLGEILFNKLKIPAIKKTATGQASTAEDVLQELALDYPLPKLLLEHRSLAKLKS
                     TYTDKLPHMVNSATGRVHTSYHQAGAATGRFSSTEPNLQNIPIKTAEGRRIRQAFIAP
                     KGNKIVAADYSQIELRIMAHLSQDAGLLNAFSSGQDVHRATAAEVFGVPLTQVSQDQR
                     RSAKAINFGLIYGMSAFGLAKQLHVGRGEAQKYIDLYFARYPGVKRYMDETRALAYEQ
                     GYVSTLCGRRLYLPDIKSNDRARRQGAERTAINAPMQGTAADIIKMAMVTVDNFLHQE
                     QLNARIIMQVHDELVFEMEESLVADLIPKICTLMSGVLTLDVPLLVEAGVGDNWDEAH
                     "
BASE COUNT          718 a          786 c          757 g          763 t
ORIGIN      
        1 cccagtcagt gcaggaatta cctccagcca cataaaaaga tgctgaatag ttacatagaa
       61 acaattaatg cgcctcatcc caattatccc ctacacccgc ctctaccaat aagggcacat
      121 ccagagttaa cacgccactc atcagcgtgc agattttggg aattaaatcg gccaccagag
      181 attcttccat ctcaaatacc agctcatcat gcacctgcat aataatgcgc gcgtttaact
      241 gctcttggtg caggaaatta tccaccgtca ccatggccat tttaataata tccgccgccg
      301 tgccctgcat gggcgcgtta atggcggtgc gctcggcgcc ttggcggcgg gcgcgatcat
      361 tacttttaat atccggcaaa tacaggcgcc gcccacataa agtacttaca taaccctgct
      421 cgtaggctag ggcgcgggtt tcgtccatat aacgttttac ccccgggtag cgggcgaaat
      481 acaaatcgat atatttttgc gcctcgccgc ggcccacatg taattgtttg gctaaaccaa
      541 acgcactcat accgtaaatt aagccgaaat taattgcctt ggcgctgcgg cgctgatctt
      601 gcgacacctg agttaagggc acgccaaaca cttcagcggc agtggcccgg tgcacatcct
      661 ggccgctgct aaacgcattt aataagcctg catcctggga gagatgggcc ataatgcgca
      721 gctcaatttg cgaataatcc gccgctacaa ttttgttgcc cttaggggca ataaacgcct
      781 ggcgaatgcg gcggccctcg gcagttttaa tggggatatt ttgtaaattc ggctcggtgg
      841 aggaaaaccg gccggtggca gcaccggcct ggtgataaga agtgtgcacg cggccggtgg
      901 cactattcac catatgcggc agtttatcgg tataggtgga ttttaatttg gcgaggcttc
      961 tgtgctctaa caataattta ggcagcgggt aatccagcgc caattcctgc aatacatcct
     1021 ccgccgtaga ggcctggcct gtggcggttt ttttaatggc ggggattttt aatttattaa
     1081 agagaatttc tcccagctgt ttaggcgagc ttagattaaa ttcctccccc gccaattcaa
     1141 acgcctcttt ttgcagctgg tccaactgag cggccaactg caaactctgc tgctgcaata
     1201 atttagcgtc aattaaagcc cccgtgcgct caatacgttg cagcaccggc agcagcggta
     1261 tttcaatttg gttatacaca tttaacagcc cggcatcctg ggcaatttta gggtacagag
     1321 tttggtgcag gcgcagggta atatcggcat cttccgccgc atagggggcg gctttttcga
     1381 tggcaatttg attaaaggta agcgctttag cgcctttgcc agctatgtct tcaaaatgaa
     1441 tggtctgccg ctgcaaataa atttccgcta aagaatccat atcctgccgg gtggtggaat
     1501 tcaccacaaa accttctaat aaggtatcaa aggctatgcc ctgcatatga ataccggcat
     1561 tggccagcac atgcacatcg tatttactgt gttggccgag ttttttaatc tgcgggtttt
     1621 ccagcagcgg ttttaaagcg cccaatacct gctcttgggt taattgtgcg ggcgcaccta
     1681 aataatcgtg gccgaagggc acataggcgg cctcgcccgg cgtgcaggca aaagacacac
     1741 cgacgatttt agcctccata taattaaggc tggtggtttc ggtatcaaaa gcaaaatact
     1801 ccgcctgctt aagttttgta atccacgcgt ttaattcttc caacgtaaat acacactgat
     1861 aattaatact gggctcagcg gtttgcacct gctcctcacc tggattttcc tcaacaggag
     1921 catttgcgct gggtgcggca tttaattcat taatccagga tttaaattcc aagcgctcat
     1981 agagggcttt taaggtggcg ttatcggcag gctttgcgtg taaatcagcg aggctaaagg
     2041 gcagttcaca atcggttttt atggtggcta attgataact taagcgcgcc atggcctcgt
     2101 gctcgcgcag tttttccgcc aatgatttag cgccgcgaat gggcaggccc gccacttcat
     2161 caaggcgttg gtataccgcg tctaaattac ctaattggct taacagcgcc tgggcagttt
     2221 tttcccctac ccccggcacg ccgggaatat tgtccacctt atcgcccata agggctaaaa
     2281 aatcgataat taattccggc ggcaggccga atttttccac cacaccggct tcatccatgc
     2341 gggtttccgt catggtgttg tgcaagctca cccaagggtt taccaattgg gccatgtctt
     2401 tatcgccggt ggagataatc actttttgcc cctgggcggc cccttggcag gccaaagtgc
     2461 caatcacatc gtccgcctcc accccttcaa tcacaatcaa cggcaggccc atggcggtaa
     2521 tggcttggtg aataggctca atctgcgccc gcaaatcatc cggcatgggg ggccggtggg
     2581 ccttgtagtg ctcgaataaa tcatcgcgaa aggttttgcc tttggcgtca aacaccatgg
     2641 caatggggct gcttgggtag tcttttaaca gccgtctcac catattgagc accagcttca
     2701 cagcgccggt gggttcgcct gcagcgttgg ttaggggcgg cgcggcgtga aaagcgcggt
     2761 agaggtagga tgagccatcg actaaaacta ggggtgctgg ttcgctcata taaagcccgc
     2821 aatcaaaaag ccgccagcat aacggatcgc cgcagcagaa tcctgcggcc aagatttttt
     2881 gagccattcg gcgcgcctta ttagcctctt ctaagctcgg ggaatgtagc cagatcgcgg
     2941 tgcagcagtg atggatacag gttattgcag accgtcaaaa agagggatct ttttgacggg
     3001 cctacacgga tgtatttacg gcgg
//
LOCUS       DAOQGS010000510         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19414_length_3024_cov_4.545975, whole genome shotgun sequence.
ACCESSION   DAOQGS010000510 DAOQGS010000000
VERSION     DAOQGS010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19414_length_3024_cov_4.545975"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2536
                     /gene="nuoG"
                     /locus_tag="PKZ52_15450"
     CDS             <1..2536
                     /gene="nuoG"
                     /locus_tag="PKZ52_15450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNG61216.1"
                     /translation="NDTRGRLVMSCMTPAADKTYIAIDEAEAKSFRKSVVEWLMTNHP
                     HDCPVCAEGGHCHLQDMTVMTGHNARRYRFTKRTHVNQDLGPFVNHEMNRCIACYRCV
                     RYYNDYAGGGDLGVFGAHDNVYFGRVADGDLESEFSGNLTEVCPTGVFTDKTHADHYT
                     RKWDMQFAPSVCQLCSVGCNTSPGERYGEIRRVENRYNGSVNYYFLCDRGRFGYGFVN
                     RTDRPVAPELKGSTQAFTIDSALDLAAEYLHSAKAVVGIGSPRASLEANFALSQLAAG
                     NISYGVGAQELQLNQLVANTLQQGLAHSPSLREIEDADGVLVLGEDLTQTAPRIALAL
                     RQAAKTRMLQLAAQKKVPSWNALPLKNIAQRDLSPIYITSIGATRLDDIAAATFRAQV
                     AEQAQLGFAVAHAIDPSAPAVTGLSVEAAQLASEIAQALLAAEKPLVVSGTALLDESL
                     VKAAINVALALKAKGKTPSLSLCLREANSLGVALLGAHTGELSLEAALEKITSGSADT
                     LLIVENDLYQRAPKALVDQALAAAKRVILVDHQRTATADAAHMLLPASSFAEGDGTLV
                     NMEGRAQRFFQVFEPSYYKDGILIREGWRWLHALHSTLNKAAVNWLHLDDATAAVAAA
                     VPALSQITQAAPGADLRIAGSKVAREPRRYSGRTSMRAAVSVHEPRQPQDADSAMAFS
                     MEGVSGPTNSSALIPFAWAPGWNSPSAWNKFQAEIGGQLRAGDSGVRLFDGQASAAYF
                     SAEVQVKSGLFLAPIYHLYGSDEQSSRSEKIQERVPQPYLRLSQTDAARLGLAEGQTV
                     SLSGPGIGVHTQLQICPDQAAGVVALPVLPGSPVVSFGINVDVQGA"
     gene            2537..>3024
                     /locus_tag="PKZ52_15455"
     CDS             2537..>3024
                     /locus_tag="PKZ52_15455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNG61217.1"
                     /translation="MEETSYFGLDALFAVVKAFIILFGVVICAAFLSFVERRLLALWQ
                     DRYGPNRAGPFGLLQLPADMVKMFFKEDWTPPFVDKLTFWLAPAIAMSGLLLAFIVIP
                     IVPGWVAADLNIGLLFFFAMAGLAVYAVMFAGWSSNNKYALLGGIRSTAQTVSYEVFM
                     GLS"
BASE COUNT          604 a          987 c          825 g          608 t
ORIGIN      
        1 aaacgacacc cgcggccgtt tagttatgtc ctgtatgacc ccggcggccg ataaaaccta
       61 tatcgccatc gacgaagctg aagctaagag ctttcgcaaa agcgtggtgg aatggttgat
      121 gaccaaccac ccccacgact gccctgtgtg cgctgaaggc ggccactgcc acctgcagga
      181 tatgacggtg atgaccggcc acaacgcccg ccgctaccgc tttaccaaac gcacccacgt
      241 caaccaggac ttagggccgt ttgttaacca cgaaatgaac cgctgtatcg cctgttaccg
      301 ctgcgtgcgt tattacaacg actacgcagg cggcggcgac ctgggtgtgt tcggcgccca
      361 cgacaatgtc tactttggcc gcgtggcgga tggcgacttg gagagcgagt tctccggcaa
      421 cctcaccgaa gtctgcccca ccggcgtatt caccgacaaa acccacgccg accactacac
      481 ccgcaaatgg gatatgcagt ttgcccccag cgtttgccaa ctctgctccg tcggctgcaa
      541 caccagcccc ggcgagcgct acggcgagat ccgccgggtg gaaaaccgct ataacggctc
      601 ggtcaactac tacttcctgt gcgacagggg ccgttttggt tacggctttg tcaaccgcac
      661 tgatcgcccg gtagcgcctg agcttaaagg tagcacccaa gccttcacta ttgactccgc
      721 ccttgatctt gcggcggaat acctgcacag cgccaaggcc gtagtcggca taggctcccc
      781 ccgcgcctcc ttggaagcca actttgccct cagccagttg gcggccggca acatcagcta
      841 cggcgtaggc gcacaggaac tgcaactcaa ccagctagta gccaacaccc tacaacaggg
      901 cctggcccat agccccagcc tgcgggaaat tgaagacgcc gatggcgtgt tggtactggg
      961 tgaagacctc acccaaaccg caccgcgcat cgccctggcc ctgcgccaag cggctaaaac
     1021 ccgcatgctg cagttggccg cccagaagaa ggtgcccagc tggaacgctc tgcccctgaa
     1081 gaacatagcc cagcgggacc taagccccat ctatatcacg agcattggcg ccacccggtt
     1141 agacgatata gcggccgcga ccttccgagc ccaagtagcc gagcaggcac aattgggttt
     1201 tgccgtggct catgccatcg accccagcgc acctgcagtt accggtttaa gtgttgaagc
     1261 cgcccagctc gccagcgaaa ttgcacaggc cctgctcgcc gcagaaaaac ctctggttgt
     1321 ttcaggcacc gccttgttag atgaaagcct ggttaaagcc gccatcaatg ttgccctcgc
     1381 gctcaaagct aagggcaaaa ctcccagcct gtccctctgc ctgcgggaag ccaatagcct
     1441 aggcgtggcc ttattaggtg cgcacactgg cgagcttagc cttgaagcgg ccctggaaaa
     1501 aatcacctcc ggcagtgccg acaccctgtt gattgttgaa aacgatctct atcagcgcgc
     1561 ccctaaagcc ctggttgatc aggccctggc tgccgccaag cgcgtgattt tggttgacca
     1621 ccagcgcact gccacggcag acgccgccca tatgctgctg cccgccagca gttttgccga
     1681 aggcgatggc acccttgtga atatggaagg ccgcgcccag cgtttcttcc aagtatttga
     1741 gcccagctat tacaaagacg gcattctgat ccgcgagggc tggcgctggc tgcacgcgct
     1801 gcacagcacc ctcaacaaag ctgccgtgaa ctggttacat ctggatgacg ccaccgccgc
     1861 cgtagccgca gcggtgcctg cgctaagcca aattacccaa gctgcccccg gcgccgactt
     1921 gcgtatcgca ggctccaaag tggcccgcga gccgcgccgt tattcaggcc gcacctccat
     1981 gcgcgctgca gtgtctgtac acgagccgcg ccaaccgcag gatgccgata gcgctatggc
     2041 cttctccatg gaaggcgtct ccggccccac caactcctcg gccctgattc cctttgcctg
     2101 ggctcccggc tggaactcac cctccgcttg gaacaaattc caagcggaaa tcggcggcca
     2161 gctgcgcgcc ggcgattcag gtgtgcgcct gtttgacggg caggcttccg ctgcttattt
     2221 ctcagccgaa gttcaagtta aatccggctt gttcctggcg cccatttatc acctctacgg
     2281 cagtgacgag cagtccagtc gttcagagaa aatccaagag cgggtgccac agccgtattt
     2341 acgtctaagc caaaccgacg ctgcgcgcct gggccttgct gaaggccaaa cagtcagcct
     2401 gtccgggccg ggcataggtg tgcacactca gctccaaatc tgcccagacc aagccgcagg
     2461 agtggtggcc ttaccggttc tgcccggcag cccggtggtg agttttggca tcaacgttga
     2521 cgtacagggg gcctaagtgg aagagaccag ttacttcggc ctagacgcgc tatttgcggt
     2581 tgttaaagcc tttattattt tgttcggcgt ggttatctgc gcggcgtttt taagctttgt
     2641 ggagcgccgc ttgctggccc tctggcagga tcgctacggc cccaaccgcg cgggcccctt
     2701 cggtttgcta cagctgcctg cggatatggt gaagatgttc ttcaaagagg actggacgcc
     2761 ccccttcgtg gacaaactca ccttctggct ggccccggcc atcgccatga gcggcctgtt
     2821 actggccttt attgtgattc ccatagtgcc cggctgggtg gccgccgatc tcaacatcgg
     2881 cctgttgttt ttcttcgcca tggcgggcct tgcggtctat gcggtgatgt tcgccggctg
     2941 gtcctccaac aacaaatacg ccctgctcgg cggtattcgc tccaccgccc aaaccgtcag
     3001 ctacgaagta tttatgggcc tgtc
//
LOCUS       DAOQGS010000511         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19415_length_3024_cov_4.219266, whole genome shotgun sequence.
ACCESSION   DAOQGS010000511 DAOQGS010000000
VERSION     DAOQGS010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19415_length_3024_cov_4.219266"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKZ52_15460"
     CDS             complement(<1..238)
                     /locus_tag="PKZ52_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNG61218.1"
                     /translation="MADLAAADKQTGVWDGIRNFQARNFLRDKAAVGDRVFIYHCQCS
                     APGVYGIARICRAAYPDPAQFDPQSPYFDPKSKGD"
     gene            complement(316..627)
                     /locus_tag="PKZ52_15465"
     CDS             complement(316..627)
                     /locus_tag="PKZ52_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61219.1"
                     /translation="MQPIKTKAQIRAELAADVAQYLVQGGEVADIPRGVSGFRDNTNP
                     YSFQSESAPRQERTPLNDVMRTLDARKRGKLSVYKPKPKKKLITDDFGEPLRWVWVQD
                     D"
     gene            1077..1274
                     /locus_tag="PKZ52_15470"
     CDS             1077..1274
                     /locus_tag="PKZ52_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61220.1"
                     /translation="MKKLLSIFTSAHSASDVSKKDSWHTPVNEVLASVRTSDNASEAT
                     NIRASSSGLRGRLPYFPGLLA"
     gene            complement(1578..2534)
                     /locus_tag="PKZ52_15475"
     CDS             complement(1578..2534)
                     /locus_tag="PKZ52_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNG61221.1"
                     /translation="MRILMAPMEGVVDPYLRALYCQIGGIDLFVTEFIRVTHTLLPAK
                     LFLRLCPELHQPIGVPVRVQLLGSDSQALAENALRAAMLGAPGIDLNFGCPAKTVNKH
                     RGGACLLQEPTTLFEIVNAVRKATPSHLPVTAKMRLGYLARQGYVENALAIAEAGAQE
                     LVVHGRSKTDGYKPPAFWDAIGEIKAALNIPVVANGDIWDLDDFKRCRAESGCTDVML
                     GRPLLAKPDLALQIKAFLAGDDYTPLPWATIVGLIWQYHLNTLPDYPSKHIGNRLKQW
                     LMYLQRHYQQAERLFEQIKTLKCADALEAAFAPLLAEANTDN"
BASE COUNT          711 a          688 c          802 g          823 t
ORIGIN      
        1 gatcgccttt ggatttaggg tcaaaataag gcgattgcgg gtcaaattgt gcgggatccg
       61 ggtaggcggc gcggcatatg cgcgcaatac cgtaaacacc cggtgcgctg cattggcagt
      121 gatagataaa tacccggtcg cctacagcag ctttatcgcg taaaaaattg cgtgcctgaa
      181 aattgcggat gccgtcccac actcctgttt gtttatctgc agcagctaag tctgccacgg
      241 agtaaacctg aggttcggtt ttaaaaagcc aataatgcat ggagtgataa attaattgag
      301 atggaagatg gcctatcagt cgtcttgcac ccacacccag cgcaggggtt caccaaaatc
      361 atcggtgatc agtttcttct tgggtttggg cttgtagacg gacaatttgc cgcgtttgcg
      421 cgcatctagg gtacgcatta catcattgag cggagtgcgt tcctgccggg gggctgattc
      481 gctctgaaaa ctataggggt tggtgttgtc gcgaaaccca gaaaccccgc ggggtatatc
      541 cgcaacctcg ccgccctgaa ccagatattg ggctacatcg gccgcgagct cggcgcgaat
      601 ttgggctttg gttttgatgg gttgcatagc ggcggcaaag aatataagcc ctcaagacta
      661 accttgttat agcccactgg gaagcattgt gactaatttt tacgagccta gccggtcgct
      721 ggcgcttacg gctgagaaaa atatttggaa ggcccagagt tgttaccatt gtgttaccga
      781 ttgagtaatt ggatgtaact ttagtatcca atctcatccg caagtgaggg cgaataaata
      841 ccctccaatt catctcgtgt gcaatgaaac cgctgtctta atgactagat ctggcttggc
      901 gtcgcaaagg tgttatttgc tgctgtgcga aaaaattata agaaatggca aaataatact
      961 tgctatcctc aaacgaggtg ttaccatttg taacgtgaag taacaaatct tcacaagcaa
     1021 gccggctgct tacccggcag atcggtacaa ctgaccttag ttagaggaaa ctttccatga
     1081 aaaagttact gtccattttc acttctgcac actctgcaag tgatgtttca aaaaaagata
     1141 gctggcatac ccccgttaac gaagttttag cctctgtgcg aacttctgat aacgccagtg
     1201 aagcgacaaa cattcgagcc agttcttctg gtttgcgtgg tcgactacct tactttcctg
     1261 gcttgctggc ctgataaggt tctagagttg cgtcttcggg cgcaactcgt ttccacctcg
     1321 tttgattggg cccattgttt gggcgctatt cggcgaagaa ttattaagct ccaattgttt
     1381 ttacccagat acatgtttta gttacccctt cggcttgtgt taatggcaag actcggcggg
     1441 tggtaaataa agggcaaccc ttattatcaa gcgctggcct gaagtggcgc tacaagaggt
     1501 tgggctggag cgacggtgtt ggcgcgcaat ccggcggcag ggacgcctaa aatacggttt
     1561 tacctgtagt ttctgcttta attatctgtg ttggcttctg ccagcaatgg cgcaaaagcc
     1621 gcctctaggg catcggcgca tttgagtgtt ttgatttgtt caaataatcg ttctgcctgc
     1681 tgatagtggc gctgtaaata catcaaccac tgttttaaac gattgcctat gtgtttactt
     1741 gggtagtcgg gcagtgtatt taagtggtac tgccaaatca ggccgactat tgtcgcccag
     1801 ggcagtggtg tgtagtcatc acccgctaga aaggccttta tttgtagcgc aagatcaggt
     1861 ttagccaata gtgggcggcc cagcattaca tccgtgcagc ctgattcggc gcggcagcgt
     1921 ttgaaatcgt ccaaatccca aatatcgcca ttcgccacaa cggggatatt aagtgctgct
     1981 ttaatttccc caatagcgtc ccaaaaggcg ggtggtttat agccatcggt tttacttctg
     2041 ccgtgcacca ccagttcttg tgcgcccgct tcggcgatag cgagcgcgtt ttccacataa
     2101 ccttggcgtg ctaaataacc caaccgcatt tttgcggtta cgggtagatg actaggagtg
     2161 gctttgcgca cggcgtttac tatctcgaaa agggttgttg gctcctgcag aagacaggcg
     2221 ccgccgcgat gtttgtttac ggttttggcc gggcagccga agttgaggtc tatgcctggg
     2281 gcgccgagca tggcggctcg aagggcattt tcagccaagg cttggctgtc actgccaagt
     2341 aattgtaccc gtaccggtac gccgattggt tggtggagtt cggggcacag acgcagaaac
     2401 aatttcgccg gcaatagcgt gtgtgttacc cgaatgaatt cagtaacaaa caggtctatg
     2461 ccgccgatct ggcagtagag ggcgcgtaaa taggggtcaa ctacaccttc catgggtgcc
     2521 atcagaattc gcatactgat cctgtgaata aaagtggttt gatcatttga ggtaagtttt
     2581 ggcgggggca gtgtaccact tgaggccttg cggattggga ttgggcttgc acaagactta
     2641 gccaaagagt cattttgcac tgcaaaatcc ctgtaattgc agcattttga cgtggttttt
     2701 cttgctcttt gccgggttaa ttcattatcg tatgcgcccc tatagcccct ggcaaaaatc
     2761 agtactcgct ggtgcccggg atcgatggta ggttgagtgg aattctgcat ggttagtcct
     2821 aagctttcgt acacacatgc cgctaattgg ctggcggttg cgggctggac gataggttgt
     2881 gccatggcaa tttcttgtaa atcaaacaac agtttttgcg cgatggaatt aaaaaaggca
     2941 gtgtaatcac tgcctttttt agttgtgtcg cgctgcaaaa atatcagtca ccaaggggtg
     3001 gctgctcact ttggagaaaa aaat
//
LOCUS       DAOQGS010000512         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19427_length_3023_cov_5.365566, whole genome shotgun sequence.
ACCESSION   DAOQGS010000512 DAOQGS010000000
VERSION     DAOQGS010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19427_length_3023_cov_5.365566"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKZ52_15480"
     CDS             <1..768
                     /locus_tag="PKZ52_15480"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNG61222.1"
                     /translation="TAGSVDLAGILLKTAAYGMMRFAIPLFPNASAEIAPLAMWLGVA
                     GIIYGAFVALRQDDIKRLIAYSSISHMGFVLIGIYSFNALALQGVIVQMLAHGLSAAG
                     LFIMSGQLYERLHTRDLNQMGGLYARMRALPPIAMFFAAASVGLPGLGNFVGEFLILL
                     GAFKVNALVTMFAASGLVLGAVYTLMMVQRSIHGAPKSDAPVVDLSWRELSMMLSLMA
                     LLLGLGLYPQPVLDTSSAVMHGVSHAVKSALPLTLAP"
     gene            776..2242
                     /gene="nuoN"
                     /locus_tag="PKZ52_15485"
     CDS             776..2242
                     /gene="nuoN"
                     /locus_tag="PKZ52_15485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNG61223.1"
                     /translation="MTFTVQHLQALLPLIITCLTCVVVMLAIAAKRHHTFNTLVCLLG
                     LNLALVATYWAAKVIPLSVTPLFIVDGYALFFTALILVSGLGSATLINTYIDSFKYNR
                     EEIYLLMVVATAGAIVLACAHHFAALFIGLELLSVSLYGLVAYSFHRPRSLEAAIKYL
                     VLSATASSILLFGIALLYAQFGSLAFADIGPKLTADAHPIVLVALGMIVIGLGFKLSL
                     APFHVWTPDVYEGAPAPVGAFLATTAKVAVFAVVVRFMLIEAINSLRIPSIFVLLAVI
                     AVISIIVGNLLALRQNNIKRILGYSSIAHFGYLLVLVLTGGTKLGLEGFAAYLAIYVV
                     TALAAFGVVTLMSKSGQNRDADHLADLRGLFWRRPYLAAIMTIAMLSLGGVPITAGFI
                     GKFYVITASITGGIWGYIMLAAVVAGSAIGIYFYLRCMINLFLTKPGMHKFEAQPGWN
                     QHTSGLMVLCSSLLIIYIGVYPETLMQFVQMAKSTGLH"
     gene            2388..2768
                     /locus_tag="PKZ52_15490"
     CDS             2388..2768
                     /locus_tag="PKZ52_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG61224.1"
                     /translation="MTSAPITYIEIPSRNLTASKTFFKQTFGWLYKDLNSDYCHITNA
                     GIPCAFYRTQKITQAGVLLVLQVSQLETAQANVIAAGGQIIKPIFNFPGGQRFHFVDP
                     SGNEFGVWAARKNTDGNNDGGQMR"
BASE COUNT          644 a          904 c          684 g          791 t
ORIGIN      
        1 accgcaggct ccgtggactt agccggtatt ctgttaaaaa ccgccgccta cggcatgatg
       61 cggtttgcca tccccctctt ccccaacgcc agcgcagaaa tagcaccgtt ggccatgtgg
      121 ttaggtgtgg cgggcatcat ttacggcgct tttgtggccc tgcgccagga cgacatcaag
      181 cgcttaatcg cctactcgtc tatctcccac atgggctttg tgttaatcgg catctactcc
      241 tttaatgccc tagcgctcca gggcgtaatc gtgcagatgc tggcccacgg tctatcggcc
      301 gccggtttat ttatcatgtc cggccaactc tacgagcgcc tgcacacccg cgatctcaac
      361 cagatgggtg gcttatacgc ccgcatgcgc gccctgcccc ccattgccat gttcttcgcc
      421 gctgccagcg tcggcctacc tgggctgggc aatttcgtcg gtgagttttt aattttgtta
      481 ggtgccttta aggtcaatgc actggtaacc atgttcgcag cctcaggcct agtgctcggc
      541 gccgtgtaca ccctcatgat ggtgcagcgc tccatccacg gcgcgcccaa aagcgacgct
      601 cccgtggtgg atttatcctg gcgggaactg agtatgatgc tgagcctgat ggccctcttg
      661 ttgggactgg gcctctatcc gcagcccgtg ttagacacct cctccgccgt tatgcacggt
      721 gtttcccacg cagtgaaaag tgcactgcca ttaacacttg ctccttgagg tcgccatgac
      781 attcactgtt caacacttgc aggctttact gccactgatt atcacctgct tgacctgtgt
      841 ggtggtcatg ctggccattg ccgccaagcg ccaccacacc tttaacacac tcgtgtgcct
      901 gttaggcctc aaccttgcgc tggtggccac atactgggcc gccaaggtga tccccctatc
      961 ggtaacgccc ttgtttatcg tcgatggtta tgccctgttt ttcaccgctt tgattctggt
     1021 ctccggcctc ggcagcgcaa ccctcattaa tacctatatc gacagcttca aatacaaccg
     1081 ggaagaaatt tacctgctga tggtggtggc cactgcaggc gccatagtcc tagcctgtgc
     1141 ccaccatttt gccgccctgt ttatcggcct tgagctatta agcgtatccc tctacggcct
     1201 ggtggcctat tccttccacc gcccgcgctc cctggaagcc gccattaaat acctagtctt
     1261 atccgccacc gcatcctcta ttttgctgtt cggtatcgcc ctgctctacg ctcaatttgg
     1321 cagtctcgcc tttgccgaca taggccccaa actcaccgcc gatgcccacc ccatagtgct
     1381 ggttgccctg ggcatgatcg tcattggctt aggctttaaa ctctccctgg ccccctttca
     1441 cgtctggacg cccgatgtgt acgaaggcgc acctgcccca gtgggcgcct ttttggccac
     1501 caccgccaaa gttgccgtat ttgccgtggt tgtgcgcttt atgctgattg aagccatcaa
     1561 tagcctgcgc attcccagta tttttgttct gctggcggtg attgcggtta tttctataat
     1621 cgtcggcaac ctgttggccc tgcgtcaaaa caacatcaag cgcatactcg gctattcctc
     1681 catcgcccac ttcggttatc tcttggtatt ggtattaacc ggcggcacta aattaggttt
     1741 agaaggcttc gccgcgtacc tggccatata cgtagtaact gccctagcag catttggcgt
     1801 agttaccctc atgtccaaat ccggccaaaa ccgcgacgcc gaccatctgg ctgacctgcg
     1861 cggcctgttc tggcgccgcc cttaccttgc cgccatcatg accatcgcca tgttatccct
     1921 cggcggtgta cccattaccg caggttttat tggtaagttt tacgtcatca ccgccagtat
     1981 caccggcggc atctggggct atatcatgtt ggccgctgta gtagcaggct ccgccatagg
     2041 tatttatttt tacctacgct gcatgattaa tttattcctc accaaacccg gcatgcataa
     2101 attcgaagcc caacccggct ggaaccaaca caccagtggt ttaatggtgc tgtgttcatc
     2161 cctgttgatt atttatatag gtgtgtaccc cgaaacactg atgcaatttg tgcaaatggc
     2221 aaaatccacc ggcctacact aagcgtccca aaatcccggc aaatgccggg atttttttta
     2281 tattcccctc cgctggaggg gttccccgca ggggcggggt ggttttggtt tgcactctga
     2341 aatttcaact tgctcctgct ttccaccgct gcccgtataa attaaaaatg acttccgcac
     2401 ccatcaccta catcgaaatc cccagccgca atctcacagc gagcaaaaca ttttttaaac
     2461 aaaccttcgg ctggttgtat aaagatttaa acagcgatta ttgccacatc acaaacgccg
     2521 gtatcccctg cgccttttac cgtacccaga aaataaccca agccggagtt ttattagtac
     2581 tgcaagtaag ccaattagaa accgcccaag ccaatgtcat cgccgcaggc ggccaaatca
     2641 taaaacccat atttaatttc cctggcgggc agaggtttca ttttgttgat ccgagcggaa
     2701 atgaatttgg agtttgggcg gcgaggaaaa acaccgatgg gaataatgat ggggggcaaa
     2761 tgcgttagga actccctatt acttataagg gctgtatcta cgcaccaagt gttatcctcc
     2821 aagaaggcct gtgcccaaac aaaacgcacc gtctcatgtg tttttttaaa aacccctatc
     2881 cattcagcct accgaccgct gaaatttatt ggccctcaaa ttgctcccaa taaaattatc
     2941 ccgcccagat aaacagagtc tgcatatatt tgggttgact aaatcacacc aaccagaaag
     3001 aaaatcgaag gtccgtcata aat
//
LOCUS       DAOQGS010000513         3012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19551_length_3012_cov_5.121745, whole genome shotgun sequence.
ACCESSION   DAOQGS010000513 DAOQGS010000000
VERSION     DAOQGS010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19551_length_3012_cov_5.121745"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..528)
                     /locus_tag="PKZ52_15495"
     CDS             complement(112..528)
                     /locus_tag="PKZ52_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61225.1"
                     /translation="MHQLEIYRLQFGLNGNKIKLASIEERRAPIPRGVPHPAEPVATG
                     IIGFWVDVLDARQQTIYRQFINKGLPINADFTCRDWSQLRRKKFRVEVQIPVLYNAHS
                     IVLFEQYLAAPEDRSLQRRKHLTAFLPQKGVCSQAV"
     gene            complement(650..1015)
                     /locus_tag="PKZ52_15500"
     CDS             complement(650..1015)
                     /locus_tag="PKZ52_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61226.1"
                     /translation="MLIHYFRSLPTGKLVLWCYLWWYCVTLVFYFDPSLSLWLNSIGI
                     SLVIGIALQLSVQRASGDRWQTFRLFMMPFCVSSFSSLIKNQGFWLIFPPKPLQLFCA
                     LSVCAGFILWVKYLQKSAR"
     gene            complement(1156..1425)
                     /locus_tag="PKZ52_15505"
     CDS             complement(1156..1425)
                     /locus_tag="PKZ52_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNG61227.1"
                     /translation="MQINVGSVESEVELQEVLARHLGFPEFYGKNWDAFWDAITGLVE
                     LPNQLEFIGIAQLQAVVPDACQKLRNSLRELNQEFPELGVEVIWS"
     gene            complement(1941..2795)
                     /gene="asd"
                     /locus_tag="PKZ52_15510"
     CDS             complement(1941..2795)
                     /gene="asd"
                     /locus_tag="PKZ52_15510"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048251.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNG61228.1"
                     /translation="MHPYVFIAAQYLTPQALLSRLVGALARCPWSFIKNPFIEKFIAR
                     YEVDMSQAQEENPRAYAHFNAFFTRALKPGARPICSGEAQIACPADGAISQLGEIQNG
                     RIFQAKGQDFSLLELLGGQADLAAAYQDGLFATVYLSPKDYHRVHMPLAGNLQRMVHI
                     PGDLFSVNTVTAAHVPRLFARNERVVCHFSTQAGPMAVVLVGAMIVASVETPWAGVVA
                     PTRRVIRTSHYGAAPEVSLAKGDEMGRFLLGSTAIVLLPKGAGRWRDDLVAGSKVVMG
                     EAIGQLAN"
     gene            complement(2846..>3012)
                     /locus_tag="PKZ52_15515"
     CDS             complement(2846..>3012)
                     /locus_tag="PKZ52_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNG61229.1"
                     /translation="DPARPIVTHCQAHHRSGFTYLVGKALGLTIAAYDGGWAEWGNLA
                     ETPKALAEDL"
BASE COUNT          789 a          787 c          768 g          668 t
ORIGIN      
        1 atttggcgaa agcagtagtc accactacgg tgagtcaaat tttgacaaaa aagcgctcat
       61 attcaagcat taggcggccg tagtagagtt caactgcggt ttttaggttc attacaccgc
      121 ctggctacac acgccctttt gcggcaaaaa cgcggttaaa tgtttgcgcc gctgcaaact
      181 gcgatcttca ggcgcagcta gatattgctc gaataacaca atggaatgag cgttgtataa
      241 aaccggtatt tgcacttcca cgcgaaattt tttgcggcgc aattggctcc aatcccggca
      301 ggtgaaatcg gcatttatcg gcaggccttt attgataaat tgccggtaaa tagtctgctg
      361 gcgcgcatcc aatacatcta cccaaaaacc gataataccg gtggccaccg gttccgccgg
      421 gtggggcaca ccgcgcggaa tcggcgcgcg ccgctcctcg atggatgcaa gttttatttt
      481 gttgccattt aaaccaaatt gcagacgata aatttctagt tgatgcatga cacagctcca
      541 attaattcct tcggatgccg tgcagattag agcttgccag aatatgaagc ggtgaaaaat
      601 tcacattcgg ccctgtgact ttttaccggc tcacttgcgg gtgcaacaat taacgggcgc
      661 ttttttgcaa atatttaacc cacagaataa accccgcaca gactgagagc gcacagaaca
      721 actgcagggg tttgggcgga aaaataagcc aaaaaccttg gtttttaatc agggaggaaa
      781 aactcgatac gcagaagggc atcataaaca gccggaaggt ctgccagcga tcgccgctgg
      841 cccgctgcac actcagctgc agggcaatgc caatcaccag gctgatccct atggagttca
      901 gccacaggct gaggctgggg tcgaaataaa acaccagggt gacgcagtac caccagaggt
      961 aacaccacag caccagtttg ccggtgggta ggctgcgaaa ataatggatc agcataaaaa
     1021 ggctcaacag gaataaggaa ccctgttgca cgagcaacga gcgtaccgac tagcgccgtc
     1081 agcacaagtg gctttctagc agcggcgaat gtgcaacgaa aaaagcgtcg gagcgtatgg
     1141 ccgcgttata tcctcttaac tccatatcac ctcaactcca agttctggga attcttggtt
     1201 taactcccga aggctatttc tgagtttttg gcacgcatca ggaacaacgg cctgcaattg
     1261 agcaataccg ataaattcta attgattcgg tagctccact agcccggtaa tggcgtccca
     1321 gaatgcatcc cagtttttac cgtaaaactc gggaaaccca agatgccgtg ccagaacctc
     1381 ttgaagctct acctctgatt caaccgaccc aacatttatt tgcatgttct agagctttac
     1441 cggctagttg aaatggtgag gaagcacttg tttcgagatg cgattgttaa aaaaatgcaa
     1501 gagccctcgt tgttaattac ccgacttaaa agacgggaga acgcaagaat acgggccata
     1561 aatgtaatca cacttaaaga atcaaacgat aactatttag ccgtttttca tacagctaga
     1621 gttatttcag gcaaagacgg gaactgtccc cggcgagttt taatgctgta acccattagt
     1681 acttcgcccg cagcggtaac tacaaattaa tgcagacacg ccatgaatac ttccctgtag
     1741 gctcgtcaaa aaggttcctc tttttgacgg tctgcaccaa tctgtagcca cccctgctgc
     1801 accgcgaatt gattaggtcg cgtggctaaa tccccccaga atttgccgcc cctacgtaag
     1861 agggaaataa tgtgtgctgg ataattgcat ttaattaaac accttcgcgc tcgtgtaatt
     1921 cattggcaaa caagggcgta ttaatttgcc agctgcccaa tagcctcccc catcaccact
     1981 tttgagcctg cgactaaatc atcccgccag cggccagcgc ccttgggcag caggacaatt
     2041 gcggttgagc ccaataaaaa acgccccatt tcatcgccct tggcaaggga aacttccggc
     2101 gcggcgccgt aatggctggt gcgaattacc ctgcgggtgg gcgccaccac gccagcccag
     2161 ggggtttcca cactggccac aatcatcgca cccaccagca ccaccgccat ggggccagcc
     2221 tgggttgaaa agtggcacac cacccgctcg ttgcgggcga acaagcgggg cacatgtgcg
     2281 gcggtgacgg tgtttaccga gaataaatca ccggggatat gcaccatgcg ctgaaggttt
     2341 cctgccaggg gcatatgcac ccggtggtag tccttaggcg ataaatacac cgtggcaaac
     2401 aaaccgtcct ggtaggccgc cgctaaatct gcctggccgc ctaataattc caacaggcta
     2461 aaatcctggc ccttggcctg gaagatgcgg ccgttttgaa tctcccccaa ttggctgatg
     2521 gcgccgtccg ccgggcaggc aatttgggct tccccgctgc aaatgggccg ggcgccgggt
     2581 tttaaggcgc gggtgaaaaa agcattgaag tgggcatagg cgcgggggtt ttcctcctgg
     2641 gcctggctca tatccacttc ataacgggcg ataaattttt caataaacgg gtttttaata
     2701 aaactccagg ggcagcgcgc caaagcgccc accaggcgcg ataaaagcgc ctggggagtt
     2761 aaatattgag cggcaataaa aacataggga tgcatgggct gtatccaggt aagaggtaaa
     2821 tgtgaatgtg tgtgcgtggc cgcggttata agtcctcagc cagggcttta ggcgtttcgg
     2881 ccagattccc ccactcggcc caaccaccgt cgtatgcggc aatggtaagg cccaaggcct
     2941 tgccgacaag gtaggtaaaa cccgagcggt gatgggcctg gcaatgggtg actatgggcc
     3001 tggccggatc ca
//
LOCUS       DAOQGS010000514         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19573_length_3010_cov_5.750931, whole genome shotgun sequence.
ACCESSION   DAOQGS010000514 DAOQGS010000000
VERSION     DAOQGS010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19573_length_3010_cov_5.750931"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="ccmC"
                     /locus_tag="PKZ52_15520"
     CDS             <1..513
                     /gene="ccmC"
                     /locus_tag="PKZ52_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HNG61230.1"
                     /translation="AIAGAIGLIWRTKLSFIVMRSAAPIGAFLTFAALFTGAVWGKPT
                     WGTYWVWDARITSMLILLFLYLGIIALQGAFQNQDAADRSAAWLAIVGMVNVPIIYKS
                     VEWWNTLHQPATIKFMQKSTIDPSMLYPLIIMFFAFYLFYFWSLLRATRAEILRREIK
                     TQWVSDILKG"
     gene            518..739
                     /gene="ccmD"
                     /locus_tag="PKZ52_15525"
     CDS             518..739
                     /gene="ccmD"
                     /locus_tag="PKZ52_15525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNG61231.1"
                     /translation="MNLYFADFSEFLQMGTHGPYVWSCYFLMLTVLLVLAFIPNIRLQ
                     QLKKMARSRKASANSSRTSGSDSDHASAA"
     gene            723..1187
                     /locus_tag="PKZ52_15530"
     CDS             723..1187
                     /locus_tag="PKZ52_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNG61232.1"
                     /translation="MHPLRKQRLVLVSAIVVVSSIIAILVGFAMKEYANLFYPVSKVA
                     SGEAPRGKSIRAGGCVVPGSVKKSKEMLLTTFDLTDGQASITVTYDSILPDLFAEGEA
                     AVVTGVVGGDGIMKASEVLAKHDENYTPPEVAESVKNTSKHQQACKGMEYDT"
     gene            1177..>3010
                     /locus_tag="PKZ52_15535"
     CDS             1177..>3010
                     /locus_tag="PKZ52_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNG61233.1"
                     /translation="MIPEYGNFALIIALTLAICLAIVPMAGSFTGRVLWMSAARPLAV
                     GQFVFTAIAFCILTYCFVVGDFSVKYVATNSNSLLPTYYKVSAVWGGHEGSLLLWALV
                     LCAWTLAVATFSKQLPLDVLARVLSVMGIIGIGFLSFMLITSNPFTRNLPGVPNEGGD
                     LNPLLQDFGLIIHPPMLYMGYVGFSVVFAFAIAALIGGRLDASWARWSRPWTNMAWAF
                     LTVGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGVFKSWT
                     ILLAIFAFSLSLLGTFLVRSGVLTSVHSFAADPARGTFVLVFLGLVVGSSLLLYAFRV
                     PEFRALPTYTWLSRETFLLFNNIIFVIAAVCVLFGTLYPILADFIAGAKISVGPPWFN
                     IFFAPLMSFIAALSGVGVFLNWKKTAANKLSPLLVTLGLSLFIGLCSPLFVGAKYLWG
                     AALVATLGSWVILSTLMHVWIKARQARHGLWVGINKLSLSYWGMVIAHIGFAWSLMGA
                     GLATIYTEQRDLRLAYQQKVQVGGYEFELKDVANTRGPNYTAETGDIQVYKNAATYTR
                     LHPENRQYLSGGNKMTEAAIDAGFTRDLYVALGEKLDANAWALRV"
BASE COUNT          672 a          712 c          741 g          885 t
ORIGIN      
        1 gctattgcag gtgccattgg cttaatttgg cgtaccaaat tatcgtttat cgttatgcgc
       61 tcggctgcac ctatcggcgc ttttttaaca tttgcagcac tgtttaccgg tgctgtatgg
      121 ggtaaaccca cctggggcac atactgggtc tgggatgcac gtatcacctc aatgctgatt
      181 ttattatttt tataccttgg catcatcgca ctgcagggcg cttttcaaaa tcaagacgcg
      241 gcagatcgca gcgcagcctg gcttgccatt gtgggcatgg taaatgtgcc gatcatttat
      301 aaatcggttg agtggtggaa tacgctgcat caaccggcca caataaaatt catgcaaaag
      361 tccaccatag accccagcat gctctaccca cttattatca tgttctttgc gttttacctt
      421 ttttattttt ggagcttact gcgtgcaacg cgcgcggaaa ttttgcgccg agagattaaa
      481 acccaatggg taagcgatat tcttaaaggt taatacaatg aatctttatt ttgccgattt
      541 tagtgaattc ctgcaaatgg gaacccatgg tccttatgta tggtcatgct actttcttat
      601 gctcaccgtg ctgctggtgc tggcgttcat acccaatatt cgcttacagc aattgaagaa
      661 aatggcgcgc tcgcgcaaag caagcgcaaa ttcatcacgc acctctggga gtgattccga
      721 tcatgcatcc gctgcgtaaa caacgtttag tgctcgtatc cgccatagtg gtcgtcagca
      781 gtattattgc catattggtg gggtttgcga tgaaagaata cgcaaattta ttttaccccg
      841 tatccaaggt tgcttccggc gaagcaccca ggggtaaatc cattcgcgcc ggcggctgtg
      901 tagtgccggg ctcagttaaa aaatctaagg aaatgttgct taccacattt gaccttaccg
      961 acggccaagc ttcaattacc gtcacctacg acagtatttt gccggactta tttgccgaag
     1021 gtgaggccgc tgtggttacg ggtgttgtcg gtggtgacgg gataatgaaa gcgagcgaag
     1081 tgctggcaaa acacgatgag aactacacac caccggaggt cgcggaatcg gtgaaaaata
     1141 ccagcaaaca tcaacaagcc tgtaaaggga tggaatatga tacctgagta cggtaatttc
     1201 gccctcatca tcgccttaac actggccatc tgcttagcga ttgtccccat ggcaggcagt
     1261 tttaccggca gggttttgtg gatgtctgca gcccgcccac tagctgtcgg gcaattcgta
     1321 tttaccgcca ttgctttttg catattgacc tattgctttg tcgtcggcga tttctccgtt
     1381 aaatatgtcg ccacaaactc gaacagttta ttacccacct actacaaagt cagtgccgtt
     1441 tggggtggac atgagggttc tttactgctg tgggcactgg ttttatgtgc gtggaccttg
     1501 gcagtggcaa ccttttccaa acaattaccc ttggacgttt tagcgcgggt gttgtctgtg
     1561 atgggcataa tcggtatcgg atttttatcc tttatgctca tcacctcaaa tccgtttacc
     1621 cgcaatttac cgggcgtacc taacgagggt ggcgatttaa atcctctgct gcaagatttt
     1681 ggcttaatta ttcatccgcc catgctgtat atgggttatg tgggcttctc cgttgtattc
     1741 gcctttgcca tagcggcatt aattggcggc cgcttggatg cctcctgggc ccgctggtcg
     1801 cggccctgga ctaacatggc ctgggcattt ttaactgtgg gtattgcctt gggcagttgg
     1861 tgggcctatt acgaattggg ctggggtggc tggtggtttt gggacccagt agaaaatgcg
     1921 tcatttatgc cctggctggt gggcacggcc ctaatacatt ctttagccgt taccgaaaaa
     1981 cgcggtgttt ttaaaagctg gactatttta ctggctattt ttgcgttctc tttaagctta
     2041 ttaggaacct tcctcgtgcg ctcgggcgtg ttaacctcag tgcattcatt cgccgcagac
     2101 ccagcccgcg gcacttttgt attggtattt ttagggttgg tggttggcag ctccttgctc
     2161 ctgtacgcat ttagagtgcc tgaatttcgc gccctaccca cctatacctg gctctcgcgg
     2221 gaaacatttt tactgtttaa caacattatt ttcgtgattg ccgcggtgtg tgttttattc
     2281 ggtacactgt atccaatttt agcggatttt atcgccgggg ctaaaatctc cgtcggcccg
     2341 ccttggttca atatattttt tgctccactc atgtcattca tcgccgcact ttccggtgtg
     2401 ggtgtatttc tgaactggaa gaaaacagct gccaataaac tatcgccact gctggttact
     2461 ttggggctga gtttatttat cggtttatgc agccctctat tcgtcggtgc taaatacctt
     2521 tggggcgcag ccttagtagc caccttaggg tcttgggtta ttttatcgac attaatgcac
     2581 gtttggataa aagcccgtca ggctcgccac ggcttgtggg tgggcattaa taaattaagc
     2641 ctaagttatt ggggcatggt aatagcccac atagggtttg cctggtcgct tatgggggca
     2701 ggattggcaa ccatttacac ggaacaacgg gatttacgtc tggcctatca acaaaaagtg
     2761 caggtaggcg gctatgagtt tgaacttaaa gatgttgcca atacacgcgg ccctaattac
     2821 accgctgaaa caggcgatat tcaggtatac aaaaacgctg ctacgtacac tcgcctgcat
     2881 ccagaaaatc gccagtattt atccggcggc aataaaatga ctgaagctgc cattgatgca
     2941 ggttttactc gcgacctata cgttgcactg ggcgaaaaat tagatgccaa tgcctgggcc
     3001 ctgcgcgtcc
//
LOCUS       DAOQGS010000515         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19602_length_3008_cov_3.354893, whole genome shotgun sequence.
ACCESSION   DAOQGS010000515 DAOQGS010000000
VERSION     DAOQGS010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19602_length_3008_cov_3.354893"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="PKZ52_15540"
     CDS             <1..2022
                     /locus_tag="PKZ52_15540"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNG61234.1"
                     /translation="VTGYGIRYEYGMFHQRIEQGAQVEYPDHWLRDGNPWEVERPDLT
                     RRVKFYGYTDFYYNADGKKCARWSGTADVLAVPYDMPIPGYQNDTVNTLRLWKAAATD
                     EFNLGEFNAGDYTEAVASKNQAEQITMVLYPNDASENGKELRLRQQYFLASASLQDVL
                     ADWQRDHGLNMAGFADKHCFQLNDTHPTLAVAELMRLLLDECNLEWVDAWAITSRCMA
                     YTNHTLLPEALERWPVRMLKELLPRLLDIIYEINARFLAEVALRWPGDVARQARMSLI
                     EEGHDPHVRMAYLAIVGSFSVNGVAALHSDLLQRELFKDFFELWPHKFNNKTNGVTPR
                     RWLAHCNTPLSNLITQAIGPKWISDLSELSGLKKFVDDREFARQWFDIKQANKAALAR
                     MISDSTGVNFSVDSLFDVQVKRIHEYKRQLLNVLHVIHLYDRIKRGDTARMKPRSVII
                     GGKAAPGYFLAKLIIKLVNNVAAVVNNDPAVTPLLQVVFVPNYRVSAMEVICPGTDLS
                     EQISTAGKEASGTGNMKFMMNGAITIGTYDGANIEILEAAGAENFFLFGLRVEDIPAY
                     RAVHSPNVIIDNDGDLKRVMHLLEAGHFNLFEPNLFDPIVNAIRNEYDPWMVAADFRS
                     YIDTQQKAIETYQDRDAWTRMSILNTAASGVFSSDRTIAEYAREIWQIM"
     gene            2287..>3008
                     /locus_tag="PKZ52_15545"
     CDS             2287..>3008
                     /locus_tag="PKZ52_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNG61235.1"
                     /translation="MEHPNPRYVSRLTKDTLALILAGGRGSRLYELTDWRAKPALYFG
                     GKFRIIDFPLSNCINSGIRRVGILTQYKAHSLIRHIQVGWSHFKRELGEYIEVLPASQ
                     RNSPNWYQGTADALYQNLDIIRASNAKYVLVLSGDHIYQMDYGTMLAQHTANKAKLTV
                     SCIEVPIEEAAGAFGVMTVDDKDRILRFDEKPEHPTPAPNNPAMCLASMGNYVFDADF
                     LLELLEKDATTEGSDHDFGKDI"
BASE COUNT          673 a          991 c          775 g          569 t
ORIGIN      
        1 gtgaccggct atggcattcg ctatgaatac ggcatgttcc accagcgcat tgagcagggt
       61 gcccaggtgg aataccccga ccactggttg cgcgatggca acccctggga ggtggagcgg
      121 ccggatctca cccgccgggt gaagttttac ggctacacgg atttttatta caacgccgac
      181 ggcaaaaaat gcgcccgctg gagcggcacc gccgatgtat tagcggtgcc ctacgacatg
      241 cccatccccg gctatcaaaa cgacacggtg aacaccctgc gcctgtggaa ggcagctgcc
      301 accgatgaat ttaacctggg ggaatttaac gcgggcgatt acaccgaagc ggtggccagt
      361 aaaaaccagg ccgagcagat caccatggtg ctctacccca atgacgccag cgaaaacggc
      421 aaagagctgc gcctgcgcca gcagtacttt ttagcctcgg ccagcctgca ggatgttctg
      481 gccgattggc agcgggacca tggcctgaac atggcaggtt ttgccgataa acactgcttc
      541 cagctcaacg acacccaccc gaccctggcg gtggctgagc ttatgcgcct gctgttggat
      601 gagtgcaacc tagagtgggt ggacgcctgg gccatcacca gccgctgtat ggcctacacc
      661 aaccacactc ttctgcctga agccctcgag cgctggccgg tgcgcatgct taaagagctg
      721 ctgccgcgct tgctggacat catctatgaa atcaacgcgc gctttttagc cgaagtggcc
      781 ctgcgctggc ccggcgatgt ggcccgccaa gcccgcatgt ccctgatcga agaaggccac
      841 gacccccatg tgcgcatggc ctacttagcc atagtgggca gcttctccgt caacggtgtg
      901 gcggccctgc actcggacct attacagcgg gagctcttta aagacttctt tgaactctgg
      961 ccccacaagt tcaacaacaa aaccaacggc gttacccccc gccgctggct cgcccactgc
     1021 aacacgcccc tgagcaacct gatcacccag gccataggcc ccaagtggat cagcgattta
     1081 agcgagttat ctggcctgaa aaaattcgtc gatgaccgcg aattcgcccg ccagtggttc
     1141 gacattaaac aggccaacaa ggccgccctg gcccgcatga tcagcgacag caccggggtg
     1201 aatttctcgg tggattccct gtttgacgtg caggttaaac gcatccacga gtacaagcgc
     1261 cagctgctca acgtgctgca cgtgattcac ctctacgatc gcatcaaacg cggcgacacc
     1321 gcccgcatga agccgcgctc ggtgattatc ggcggcaagg cggcgccggg ttatttcctc
     1381 gccaagctga ttatcaagct agtgaataac gtggccgccg tggtcaacaa cgacccagcg
     1441 gtgaccccgc tgctgcaggt ggtgtttgtg cccaactacc gggtgtccgc catggaagtc
     1501 atctgccccg gcaccgacct ctccgagcag atctccaccg ccggtaaaga ggcctcaggc
     1561 accggcaaca tgaaatttat gatgaacggc gccatcacca taggcaccta tgacggcgcc
     1621 aacatcgaga ttttagaggc cgcaggcgcc gagaacttct tcctgttcgg cctgcgggtg
     1681 gaagatatac ccgcctaccg cgccgtgcac agccccaacg tcatcatcga caacgacggc
     1741 gatctaaaac gggttatgca cctgctggag gccggccatt tcaacctgtt cgagcccaac
     1801 ctgtttgatc ccatagtcaa cgccatccgt aacgagtacg acccctggat ggtggcggcg
     1861 gatttccgct cttacattga tacccagcaa aaagccatcg agacctacca ggatcgcgat
     1921 gcctggacgc gtatgagtat cctcaacacg gccgctagcg gcgttttctc cagcgatcgc
     1981 accatcgccg aatacgcccg cgagatttgg cagataatgt agccatcgct tcccacccta
     2041 agcgccgctg ggtaacaata gcggcgcctg cggtggggtt tggcctgcgc acacccgtcg
     2101 gcctctaacc agttcaccta ggaaggctct gcttaacgcg cacagctgcc ttttcaaaac
     2161 cctcgcgggc atggagcctg cgcctcgcca ccaggaggta ctcaggcagg tttgaaaagg
     2221 ccagttgccg cgttcatcag aggttcctta agcttccaat tgcaccttta ctctgctgga
     2281 gaccccatgg aacaccccaa cccgcgttat gtaagccgct taaccaaaga caccctcgcc
     2341 ctgatactcg ccggcggacg cggtagccgt ttatatgagc tgaccgattg gcgcgccaaa
     2401 cccgccctct acttcggcgg caaattccgc atcatcgact tccccctatc caactgcatc
     2461 aactccggta tccgccgcgt cggcattctc acccagtaca aggcccactc cttaatccgc
     2521 cacatccaag tgggctggag ccactttaag cgcgagctgg gcgaatacat cgaagtgctg
     2581 cccgcctccc agcgcaactc ccccaactgg tatcaaggca ctgccgacgc cctctaccaa
     2641 aacctcgaca tcatccgcgc ctccaacgcc aagtatgtgt tggtgctgtc gggcgaccat
     2701 atctaccaaa tggactacgg caccatgttg gcccagcaca ccgccaacaa agccaaactc
     2761 accgtctcct gcatcgaagt gcccatcgaa gaagccgctg gcgccttcgg tgtaatgact
     2821 gtggacgaca aagaccgcat cctgcgtttt gacgaaaaac ccgaacaccc aactcctgcc
     2881 cccaacaacc cggccatgtg cctagcctcc atgggcaact acgtctttga cgccgacttc
     2941 ctgctggaac tgctggaaaa agacgccacc accgaaggct ccgaccacga cttcggcaaa
     3001 gacatcat
//
LOCUS       DAOQGS010000516         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19655_length_3003_cov_4.544437, whole genome shotgun sequence.
ACCESSION   DAOQGS010000516 DAOQGS010000000
VERSION     DAOQGS010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19655_length_3003_cov_4.544437"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1417)
                     /gene="hemL"
                     /locus_tag="PKZ52_15550"
     CDS             complement(143..1417)
                     /gene="hemL"
                     /locus_tag="PKZ52_15550"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNG61236.1"
                     /translation="MNPLFAQAQAVIPGGVNSPVRAFRAVGGEPIFIERANGAYIFDT
                     DGKRYIDYVLSWGPMILGHNDPDILEAVIAKARMGLSFGAPTQIETELAELIVELIPS
                     IEKVRFVSSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGALTFGVP
                     SSPGVPKSLADHTITLNYNDSAQVEEVFSSIGDQIACVIVEPVAGNMNCIPPLPDFLP
                     TLRKVCDTSGALLIFDEVMTGFRIGIAGAQGHFNIKPDITTLGKVIGGGMPVGAFGGR
                     ADVLNHLAPLGPVYQAGTLSGNPVAMAAGLASLKKINQPGFYAPLFRRTTALVDGLQA
                     IANETGIPFTTNHCGTLWGGFFTEEGPITNYQQVIACNNTRFNQFFHGMLSGGVNLAP
                     ASYEAGFMSAAHTDEDIAETLQIARQVFSALN"
     gene            complement(1437..>3003)
                     /locus_tag="PKZ52_15555"
     CDS             complement(1437..>3003)
                     /locus_tag="PKZ52_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61237.1"
                     /translation="GGSSSSSSGGETSSSSTSSSGGSTSSSSTSSSSSGETSSSTSSS
                     STSSSSGSNSTSSSGSTSSSSTSSSSGVGSTTSSSSSGSTSSSSGGSSSSSSGGESST
                     SSSSTSSSSSSSSSSSSSSSGITRPFKLKFAQESISGTEVAAGNQKIWLYAQDGKTLV
                     GSATTNSEGWADLGQQPEQFAIAYKVGNQFIYQGVNNSGEIKVVTPFNETVELDPELP
                     NGGCPVSKAVTISGIPEEEPDDFSPEYIEVRTNHPEWVATLGDDRKTVTICAARAPLD
                     GKFDLWLSSKAGYQHIQNINWQGAAAFNYAPQAPSLSSWSLSTAASLFAANVIITNDD
                     QLAVYNELIYDVKGSLNTQLAVPKLNGASYHLVLVGQADSPVAGFKVSRNFTSLSSLP
                     AIDVPAAGIEQLTWDASAQKVTWKTNANANIDFFELEVDLGNTQTPSGLVFTTLLDKN
                     QSSFTLPQLPAEIPAPDGNRSVDLSSVDYQGYNNLSDWLGETQSPLITLQEYIEKRKE
                     AENNATRVILYKN"
BASE COUNT          672 a          731 c          868 g          732 t
ORIGIN      
        1 gcgcaatatt caatccgttg caaagcaaca cgtttttcac gataattcgc ggcctgccat
       61 ggtttcatct ggccgaaaca gtagccaata agggctccgt gcctgcaagg catgtagctt
      121 aagtagtaga aataaatagg cgctagttga gggcgctgaa tacctggcga gcaatctgca
      181 atgtttcggc tatgtcttca tccgtatggg cagcggacat aaagcctgct tcataagacg
      241 cgggggcgag gttgacgccg ccagataaca tgccgtggaa gaattgatta aagcgcgtgt
      301 tattacaggc aattacctgt tggtaattgg taatggggcc ttcctcggtg aaaaaaccgc
      361 cccaaagagt gccgcagtgg ttggtggtga aggggatgcc ggtttcattg gcgatggctt
      421 gcagcccatc aactagcgct gtggtgcggc gaaacagcgg ggcgtaaaaa ccgggctggt
      481 ttattttctt caagctggca aggcccgccg ccatagcgac tggattaccg gataaggtac
      541 cggcttggta gaccggccca agtggtgcga ggtggttgag cacatcggcg cgaccgccaa
      601 aggcgcccac cggcatgccg ccacctatca ctttgcccag ggtggtgatg tctggcttga
      661 tattaaaatg gccttgggcg ccggcaatgc cgatacgaaa gcctgtcatc acctcatcaa
      721 aaatcaacag cgccccggag gtgtcgcaga ctttgcgcag ggtggggaga aagtcgggca
      781 atggtggaat gcagttcata ttgcctgcca ctggctcaac gatcacgcag gcgatttgat
      841 cgccgatgga actgaatacc tcttcaacct gtgcactgtc gttgtagttg agggtgatgg
      901 tgtggtcagc cagggatttg ggtacaccgg gggaggacgg tacaccaaat gtgagggcgc
      961 cggaacctgc tttaaccagc agcgaatcgg agtggccgtg gtagcagcct tcgaatttga
     1021 tgattttatc gcggccggta aaacctcgtg cgaggcgaat agcgctcatg gtggcttccg
     1081 tgccagagct gacaaagcgg actttttcaa tgcttgggat gagttcaaca attaactcag
     1141 ccagctcagt ttcaatctgt gtaggtgcgc caaagcttaa acccatgcgc gctttggcga
     1201 tcacggcctc cagtatgtct gggtcgttgt ggcccagaat catgggcccc cacgagagga
     1261 cgtaatcgat ataacgcttg ccgtcggtat caaaaatata agctccgttg gcgcgctcga
     1321 taaaaatcgg ctcaccgccg accgcgcgga aggcgcggac gggggagtta acgccgccgg
     1381 ggattacggc ttgggcctgg gcaaataatg ggttcatgct tcgtgggtta accagactag
     1441 tttttgtaca gaattacgcg agtggcgtta ttttcggctt ctttgcgttt ttcaatgtac
     1501 tcctgcaggg taatcaatgg agattgcgtt tcacccagcc aatcactcag gttgttatag
     1561 ccctggtaat ccacgctgga taggtcaacc gatcggttgc cgtctggagc tggaatctca
     1621 gcaggtagct gtggcagggt aaacgacgat tggtttttat ccagcaaggt tgtgaacact
     1681 agcccactgg gggtttgtgt gttgccaagg tcaacttcca gctcgaagaa atctatgttc
     1741 gcattggcgt tggttttcca agttactttc tgcgcactag catcccaggt gagctgttca
     1801 ataccagcag ccggtacatc aatagcaggc agtgaagaca gcgatgtgaa gttgcgagat
     1861 accttgaagc cagccacagg gctgtcggct tggccgacga gaaccagatg gtaggaggca
     1921 ccgtttagct tgggcactgc aagctgggta ttgagcgaac ctttcacgtc gtaaatgagt
     1981 tcgttgtaaa cggccaactg gtcgtcatta gtaattatca cattggccgc aaacaatgat
     2041 gctgcagtac tcaaactcca gctgctcagc gatggcgctt gcggggcata gttaaacgcc
     2101 gccgcaccct gccagttgat gttttgtata tgctggtagc ctgcttttga cgataaccac
     2161 agatcaaact taccgtctag tggcgcccgc gctgcgcaga tcgtcactgt tttgcggtcg
     2221 tctcccaagg ttgccaccca ctctggatgg ttggtgcgca cctcaatgta ttcagggctg
     2281 aaatcatctg gttcctcctc tggtatccca ctgattgtca cagctttgct gacagggcaa
     2341 ccgccgttgg gtaactctgg atctaattca accgtttcat taaacggtgt gaccactttg
     2401 atttcacctg agttgttaac accttgatag atgaactggt tgccaacttt ataggcaatg
     2461 gcaaattgct caggctgttg ccctaaatcc gcccagcctt cgctgttagt ggttgccgag
     2521 ccgacgaggg ttttaccatc ctgcgcgtaa agccagattt tttggttgcc tgcagccact
     2581 tcggtgccgg aaatgctctc ctgtgcaaac ttgagtttga atggacgagt gatgccagag
     2641 cttgagctac tggacgagct tgagctgctg gagctagagg aagtgcttga gcttgatgta
     2701 ctggattctc cgccagagga gctggagctc gaaccacctg aagagcttga tgttgagccg
     2761 ctggagctgc tggaagtagt agatcctacg ccgctacttg aagaagtcga actgctggag
     2821 gttgaaccac tgcttgacgt gctgttggaa cccgatgagc tgctggtgct gctagaagag
     2881 gtactgctgc tagtttcgcc gcttgagctg ctggaagtag acgaactgct ggtgctgccg
     2941 ccactggagg atgtgctaga gctactggtt tcaccgcctg agctactgga agacgaaccg
     3001 ccg
//
LOCUS       DAOQGS010000517         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19790_length_2990_cov_4.604429, whole genome shotgun sequence.
ACCESSION   DAOQGS010000517 DAOQGS010000000
VERSION     DAOQGS010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19790_length_2990_cov_4.604429"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKZ52_15560"
     CDS             <1..771
                     /locus_tag="PKZ52_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG61238.1"
                     /translation="PARETLGEQPVRLQVNNLSVKDDQLLDAIKNISFSIRGGEILGI
                     AGVSGNGQKQLVEALAGQRPKASGEINVAGRDYLPTQERALAEGFYCLPEEPLRNACV
                     PNMSLEDNLSLRQFNQPAFSRWGWLLNRKAIRKYAVEKIAEYAVRTQGPGQMIKGLSG
                     GNVQRVVLARELAANVNILVVANPCFGLDFKAVADIRGRILMARNQGAAVLLLSEDLD
                     EVLALSDRLYVISGGQLPYETTPQQADLTTIGQYMAGH"
     gene            797..1477
                     /locus_tag="PKZ52_15565"
     CDS             797..1477
                     /locus_tag="PKZ52_15565"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HNG61239.1"
                     /translation="MSAIAARPFAFDLTAKKPALIIIDMQRDFLEAGGFGAALGNDVS
                     LAAACIPAVQALLTAARALQLPIIHTRECHRADLSDCPEHKRVRGSSALRIGDAGPMG
                     RILIDGEPGNAIIASLAPMPGELELTKPGKGAFYNTQLQEYLQQQGIEDLIFAGVTTE
                     VCVQTTMREANDRGYRCLLVEEATASYFPQFKAACIEMLVAQGGIVGWAAQLEAVLAG
                     LNKAYGHE"
     gene            1470..1817
                     /locus_tag="PKZ52_15570"
     CDS             1470..1817
                     /locus_tag="PKZ52_15570"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNG61240.1"
                     /translation="MNNIIWLPNIFDIQSLLTQCQWQPLVEGVRFAEIYSTASPGPRA
                     AFLHYLPGAKVPPHMHQGLEHILILQGYQIDGDKTYGTGALVIHPAGTVHALESPEGC
                     VALGIWEQPVAFV"
     gene            2024..>2990
                     /locus_tag="PKZ52_15575"
     CDS             2024..>2990
                     /locus_tag="PKZ52_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNG61241.1"
                     /translation="MELFFGIDLGTSNSAIACFDGTELSVVPNSRGEGNTPSVVRFTE
                     NSVVVGAKAQNFLFKDPLHTHKEFKRLMGTQVKTKPDQFGHQYTAEQLSSEILKALRQ
                     DAIKFKDCAPDHIVITVPALFELPQSNATAEAARLAGFTQVELLPEPVASALAAGWSE
                     QENAQAWLVFDLGGGTFDVSLVESRDGLLRVVGHDGDNFLGGRDIDRLLAEWVINKLH
                     AEHGVQINQDQAEHTSIVRHIYQEVEKAKIRLSSQERTSIELDFEWDDDAFEADFLIA
                     RSELEELITPIVERAIEVCKRLISQQGLSLAQISRVVLVGGPAHMP"
BASE COUNT          731 a          704 c          816 g          739 t
ORIGIN      
        1 ccagctcgcg agaccttagg ggagcaaccg gtgcgcttac aggtgaataa tctatcggta
       61 aaagacgacc aattattaga tgccattaaa aatatcagct ttagtattcg cggcggagaa
      121 attctcggta ttgccggtgt gtctggcaat gggcaaaaac aattggtgga ggctttagca
      181 ggccagcggc ctaaagccag tggtgaaatt aatgtagccg ggcgggatta tctgcccact
      241 caagagcggg cactggcgga aggtttttat tgtttgccag aggagccact gcgcaatgct
      301 tgtgtgccga atatgagctt ggaagataat ttaagcctgc ggcaatttaa tcaacccgca
      361 tttagccgtt ggggttggct gctcaatcgc aaagcgattc gcaaatacgc ggtggagaaa
      421 attgccgaat acgctgtgcg cactcagggc ccagggcaaa tgattaaagg cttgtccggt
      481 ggcaatgtgc agcgggtggt attggcgcgc gagctggcgg caaatgtaaa tattctggtg
      541 gtggccaacc cctgttttgg cttggatttt aaagcggtag cggatattcg cgggcgtatt
      601 ttaatggccc gcaaccaggg tgcggcggtt ttattattaa gcgaagattt agatgaagta
      661 ttggcgctga gtgatcgctt atatgttatt tccggcgggc aactgccgta tgaaaccacg
      721 ccgcaacagg cggatttaac caccatcggc caatatatgg ctggccacta atttttgatt
      781 aaaggccgga ggatttatga gcgcaattgc ggcacgcccc tttgcatttg atttaaccgc
      841 aaaaaagccg gcattaatta ttattgatat gcagcgggac tttttagaag caggtggctt
      901 tggcgcagcc ctgggcaatg atgtgagcct ggcggcggcc tgtattcccg cagtgcaggc
      961 gttattaact gccgcgcggg cattacaatt accgattata cacacccgcg aatgccaccg
     1021 ggcggattta tccgattgcc ccgaacacaa acgcgtgcgc ggcagcagcg cactgcgcat
     1081 tggcgatgct ggccctatgg ggcgtatttt aattgatggt gagccgggca atgccattat
     1141 tgccagcctt gcgcccatgc ctggggaatt agaattaact aaaccgggca agggggcatt
     1201 ttacaatacc cagctgcagg aatatttaca gcagcagggt attgaagatt taatttttgc
     1261 cggtgtaacc accgaggtat gtgtgcaaac caccatgcga gaggccaatg atcgcggtta
     1321 ccggtgttta ttggtggagg aagccacggc cagttatttc ccgcaattta aagccgcttg
     1381 tattgaaatg ctggtggcac agggcggtat agttggctgg gcggcacaac tggaggccgt
     1441 gttggccggt ttaaataagg cgtatggcca tgaataatat tatttggctg cccaatattt
     1501 ttgatattca aagtttatta acccaatgcc aatggcaacc cctagtggaa ggtgtacgct
     1561 ttgccgaaat ttacagcacg gcaagcccag gcccacgggc ggcattttta cactatttgc
     1621 ccggcgccaa agtgccgccc catatgcacc aggggttgga gcatatttta attttgcagg
     1681 gctaccaaat tgatggcgat aaaacctacg gcactggcgc gcttgttatt cacccggcgg
     1741 gcactgtcca cgcgttggaa tccccagagg gttgcgtggc cctgggtatt tgggagcagc
     1801 cagtagcgtt tgtatagccc cctaaacgcc gtaggattaa tcttccgccc tcggggcacc
     1861 tgcttgcccc catcacaccg cttaccacag gcccatgggc cgtgcgggta ggcgcaccta
     1921 aatcacagcc ctacaggcgc tttcccccac ccaatggctt ttaagcagac ggaacgtcta
     1981 tacttcgccg gtctaattta ttgaggttaa ttgaggccag cccatggaat tgtttttcgg
     2041 tatagatctt ggaacctcca attctgctat cgcgtgtttt gatggcacag aattaagtgt
     2101 ggtgcccaat agccgcggtg aaggcaatac cccctcggtg gtgcgtttta cagaaaactc
     2161 agttgtggtg ggtgccaagg cgcaaaattt tttatttaaa gacccgctgc acacccacaa
     2221 agaatttaag cgtttaatgg gcacccaggt taaaaccaag cccgatcaat ttggccacca
     2281 atacacggcc gagcaattat ccagcgaaat attaaaagcc ctgcgccaag acgctattaa
     2341 atttaaagac tgcgcaccgg atcatatagt aattaccgtg cccgctttat ttgaattgcc
     2401 ccagagtaac gccacggcag aggccgcccg tttggcaggc ttcacacagg ttgaattatt
     2461 gcccgagcca gttgcctccg ccctggccgc aggctggagc gaacaggaaa atgcccaggc
     2521 ctggttagta ttcgatttag gcggcggcac ctttgatgtt tccttagtgg aatcccgcga
     2581 tggtttattg cgcgtggtgg gccatgatgg cgataatttt ttaggcggcc gggatataga
     2641 ccgtttatta gccgaatggg tgattaataa attgcacgct gaacacggcg tgcagattaa
     2701 tcaggatcaa gccgaacaca catctatagt gcggcatatt tatcaagaag tggaaaaagc
     2761 caaaattcgt ttatccagcc aagagcgcac ctccattgaa ttggattttg aatgggatga
     2821 cgatgcgttt gaagcggatt ttttaattgc ccgcagtgag cttgaagaat taatcactcc
     2881 catagtggag cgcgctatcg aggtatgtaa gcgtttaatt agccagcagg gcctaagcct
     2941 tgcgcagatt agccgcgtgg tattagtggg cgggcctgcc catatgccaa
//
LOCUS       DAOQGS010000518         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19828_length_2986_cov_4.555101, whole genome shotgun sequence.
ACCESSION   DAOQGS010000518 DAOQGS010000000
VERSION     DAOQGS010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19828_length_2986_cov_4.555101"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKZ52_15580"
     CDS             <1..535
                     /locus_tag="PKZ52_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNG61242.1"
                     /translation="ANAFPPALVLPLDEDPRLAFVCHWLFDEAFAELEGRQLAINRLA
                     ELLVLQMLRHLVERASLQTGMLAGLAHPHLHRALLAIHSNPAHSWSLMELASLSGMSR
                     SKFAEEFKTVLGQPPGDYLIEWRLGVAQSLLQKLKPVGLVAHEVGYENASVLARVFRK
                     KLGLTPLEWLERQRELG"
     gene            complement(653..2020)
                     /locus_tag="PKZ52_15585"
     CDS             complement(653..2020)
                     /locus_tag="PKZ52_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG61243.1"
                     /translation="MPVPATILQILHNNNINYAVTPTEPSPMVDKTVCQVRSQLLADQ
                     HGWVQVLLPQKDMLDLELLAQELGRTLHPVAAAEINALLAKNNLQSVPAMPAWQGLPT
                     LVDLNILRYENVCLNSGDQAELVCVQRADFQALIREANLCSLSCPTQTINSDARLDEP
                     QIEQAISAFTQRRIQQRLHETLEIPPLNDTASKIIRLRANPHADISDLAKIIEFDPSL
                     AAQVVSWAASPYYSAPGKIKSVQDAIVRVLGFDMVINLALGLSLGRAFNPQVISKAQI
                     RDYWKKAVFMAAAVEALVTSIPRISRPAFGMSYLVGLLHNFGYLILAEVFPPYFQNIN
                     RHLEANPHLAPGLVEQHLVGVASCQITAWLLEYWNLPAEVCTGIRQQFNPAYAGEHAA
                     YSRLVYVANHLLLQHGPGQLVRQPLPEEMFESLNLSREAADEAIINLLNCADDLKSLA
                     RVLEG"
     gene            complement(2307..>2986)
                     /locus_tag="PKZ52_15590"
     CDS             complement(2307..>2986)
                     /locus_tag="PKZ52_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG61244.1"
                     /translation="TRTEEQASSLEETAASMEEITQMLHKSQHNAETANSLSLQAVEI
                     AKSGDASVEKTAAAMRGIQAASTAIANIIGVIDELAFQTNLLALNAAVEAARAGEQGR
                     GFAVVAAEVRSLAQRSAHSAKEIKQLIQDSVSRVQDGSALVEVSRKTLNQMVQEILQV
                     ANMVGEIVVTTKEQASGVSQINTAILQMDQITQQNAALVEQASAASETMADQARALDQ
                     LMGFFKR"
BASE COUNT          610 a          796 c          880 g          700 t
ORIGIN      
        1 ggccaacgcg tttccgcccg ccctggtgct gcctttggat gaagatcccc gcttggcgtt
       61 tgtgtgccat tggttgttcg atgaggcctt tgccgagctg gaggggcgcc agctggcgat
      121 taaccgtttg gctgagttgt tggtgctgca gatgctgcgc cacttggttg agcgggccag
      181 tttgcagacc ggtatgttgg caggccttgc tcacccgcac ttacaccgcg ccctgctggc
      241 catccacagc aacccggccc acagttggag cctgatggaa ttggccagtt taagcggcat
      301 gtcgcgcagc aaattcgccg aggaattcaa aaccgtactg ggccagccgc cgggggatta
      361 tttaattgag tggcgcctag gggtggcgca aagcctgttg cagaagctta aaccggtagg
      421 cctggtggcc catgaggtgg gctatgaaaa tgcctcggtg ctggcgcggg tgttccgcaa
      481 aaaacttggc ctgacgcctt tggagtggtt ggagcggcag cgggagttgg gttagtagct
      541 ctgccatggg ccagtattta tgctgcagtg ggcacccaca cggggcgccc ctacagctgc
      601 ggtctaggtc gcggcgcctt gaatgggtgc cctgccgcgc ggcctgaact gcctaaccct
      661 ccaacacccg cgctaaggac tttaaatcgt ccgcgcagtt gagcaggttg ataatcgcct
      721 cgtccgccgc ctcgcgggac aggttgaggc tttcaaacat ttcctccggc agcggttggc
      781 gcaccagctg gcccgggccg tgttgcagca gcaggtggtt agccacgtac accaggcggc
      841 tgtaggcggc gtgctcgccg gcataggccg ggttaaattg ctggcggatg ccggtgcaaa
      901 cttccgccgg taggttccag tactccagca gccaggcggt gatttggcag ctggccacgc
      961 ccaccaggtg ctgctccacc aggcccggag ccaggtgggg gttggcttcc aggtggcggt
     1021 taatattttg gaaataaggc ggaaacacct ccgccagaat caaatagcca aagttgtgta
     1081 gcaggcccac caggtaggac atgccaaagg ccggccggct gatgcggggg atgctagtga
     1141 ccaaggcctc caccgcagcg gccataaata cggccttttt ccagtaatcg cgaatctgtg
     1201 ctttgctgat cacttgaggg ttaaacgcgc ggcctaagga gaggccgagg gcgaggttaa
     1261 tcaccatatc aaagcccagc acccgcacta tggcatcctg caccgacttt attttgcccg
     1321 gcgccgaata atagggggag gccgcccagc tcaccacttg ggcagccagg gacgggtcga
     1381 attcgataat tttcgccagg tcgctaatgt ctgcatgggg gttggcgcgc aggcgaataa
     1441 ttttgctggc ggtgtcgttt agaggcggta tttctagggt ttcgtgcagg cgctgctgaa
     1501 tgcgccgctg ggtgaaggcg ctgatggcct gttcaatttg cggctcatcc aaacgcgcat
     1561 cgctattgat ggtttgcgtg gggcaggaga ggctgcacag gttggcctcg cggatgaggg
     1621 cctgaaaatc cgcccgctgc acacacacca attccgcctg atcgccggaa tttaagcaga
     1681 cgttttcata gcgcagaata tttaaatcca ccaatgtggg cagcccctgc caggcgggca
     1741 tggcgggcac actttgcaaa ttatttttgg ccaaaagggc attaatttcc gcggccgcca
     1801 ccgggtgcag ggtgcggcct agctcctggg ctaatagctc taaatccaac atatcttttt
     1861 gcggcaacaa aacttgcacc caaccgtgtt ggtccgccaa taactgcgag cgcacttggc
     1921 agacggtttt atccaccatg gggctgggtt cggtgggtgt taccgcgtaa ttaatattgt
     1981 tgttgtggag tatttgcaaa atggtagcgg ggacgggcat gaagaattta cctcacatca
     2041 tgcggatcaa ttgatgtctg tgacagacgg ctacctaaag catagttgcg cccaggcaaa
     2101 gcgctaggtg caagctggtg ggggattgcg caccaaaaca tcagagtttt aaaaggtgat
     2161 atgcaggtag gttggttaga cagtgcgtgg cgacagcaaa aattccctct tattcccgcc
     2221 acacgcggca gccagtgctt atagccagtt gtacagtatt gattgtgagg gattaaaacg
     2281 agaggttatt tataaattat ttcgccctag cgcttaaaaa atcccattaa ttgatctaaa
     2341 gcccgcgctt ggtcggccat ggtttcgcta gcggcggagg cctgttcgac cagcgcggca
     2401 ttttgttggg tgatttgatc catctgcaaa atggccgtat taatttggct gacgcccgaa
     2461 gcctgttcct tagttgtcac cactatttcc cccaccatat tggccacctg taaaatttct
     2521 tgcaccattt gattaagggt tttgcgggag acttccacta gggcgctgcc atcctgcacg
     2581 cggcttaccg agtcttgaat taattgttta atttctttgg ccgaatgtgc cgagcgctgg
     2641 gcaaggcttc tcacctcggc ggccaccacc gcaaaaccgc gcccctgctc cccggcgcgg
     2701 gccgcttcca cagcggcatt taaggctaat aaattggttt gaaacgccag ctcatcaata
     2761 acgccaataa tattggcaat ggcggtgcta gccgcttgaa taccgcgcat ggccgcagcg
     2821 gttttttcca ccgaggcatc gccggattta gcaatttcca ccgcttgcaa acttaaacta
     2881 ttagcggttt ccgcattgtg ctggcttttg tgcagcattt gggtaatttc ttccatactg
     2941 gcggcggttt cttctaaaga ggacgcctgc tcctccgtgc gcgtac
//
LOCUS       DAOQGS010000519         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19829_length_2986_cov_4.289662, whole genome shotgun sequence.
ACCESSION   DAOQGS010000519 DAOQGS010000000
VERSION     DAOQGS010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19829_length_2986_cov_4.289662"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKZ52_15595"
     CDS             complement(<1..946)
                     /locus_tag="PKZ52_15595"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNG61245.1"
                     /translation="MTVVVPVVLSGGSGSRLWPKSRQFYPKQLHKLYGDCTMLQHTLK
                     RVDHLGEPIVICNNEQRFMVADQVHAIGQRAQIILEPIARNTAPAIAVAALTAFKQSP
                     DAIIVVLAADHLIENTAEFRRCLDIAIEAAAGNKLVTFGVVPTKPETGYGYIKTAPGD
                     SPVKQVIQFVEKPNRELAEEYLAAGCYYWNSGMFVFPAAQLIDELTKLQPDMVAHARA
                     ALESAQSDLDFLRLGAANFEMCSNISIDYALMEHTANAWTVPLGAGWSDLGSWDAVWD
                     VAQKDDSGNSCEGDVILQDCHDCFVMADTRLVTLLGVNN"
     gene            complement(1709..>2986)
                     /locus_tag="PKZ52_15600"
     CDS             complement(1709..>2986)
                     /locus_tag="PKZ52_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61246.1"
                     /translation="DFHPRDAAFLVGVVQADGKLVLTATQPQVFASNASFDAQGNLSF
                     VLKVGGQCTQEALVTAQKAKPVNEAAALSGDSLNWFDVENLFSEQGYSSDFTYGLIAP
                     QAVESTFHYDPASKTWQDNSASHHKATVLVTEQGLVLSDDLIMLTDFGPEGEGAHLVP
                     SVAGQALAVPGMNLQLHEFDLGGQNLSQLLAPEIAKAIPPSAEFPQGAKAWVAELTQV
                     ALSAEFYCNEGFDPWFASHLECDNIVAKAWQPAGEGQPNTPVPATSLDDVVASPDEFA
                     TGKAVPVWIASSHEASGAMVNIQGYFISSDGSLNGTLSLRPVLQSQANQVPKVLADLP
                     VQKIVKAGKTLLAVNLPNELIANSDWDHEASGLFVTEEAALDNTPLVRHGHLLAAGVK
                     QQHIVYNSAARTAILSNFNPAANGPAPADQPPM"
BASE COUNT          665 a          772 c          856 g          693 t
ORIGIN      
        1 ggttattcac ccctaataga gtcaccagac gagtatctgc cattacgaag caatcgtggc
       61 aatcctgcaa aatgacatca ccttcacagc tattgcccga atcgtccttt tgagcgacat
      121 cccagacagc atcccagcta cctagatcgc tccagcccgc accaaggggg actgtccatg
      181 cgttcgctgt atgttccatt aaggcatagt cgattgaaat gttagaacac atttcaaaat
      241 ttgctgcacc gaggcgaaga aaatccaaat cgctttgggc actttccaaa gcagctcttg
      301 catgcgccac catgtccggt tgcaatttgg tcagttcgtc aattaactgc gccgctggaa
      361 atacaaacat cccactattc cagtagtaac aaccagcggc gaggtattct tctgcaagtt
      421 cgcggttggg cttttcaaca aactggatga cttgttttac aggggaatca cctggtgcgg
      481 ttttaatata accgtaacct gtctcaggtt tagtggggac cacgccgaag gtaactagct
      541 tgttcccagc ggcagcctct attgcaatat cgaggcagcg acggaattca gcggtgtttt
      601 caatcaagtg atccgctgct aatacaacaa tgatggcatc tggggactgt ttgaaggcag
      661 tcagcgcggc aactgcaatg gccggagcag tgttgcgggc gatgggttca aggatgatct
      721 gggcgcgctg gcctatggca tgtacctggt cagccaccat aaagcgctgc tcgttattgc
      781 agattacaat cggctcgcca aggtggtcta cccgttttaa agtgtgctgc aacatggtgc
      841 aatcaccata aagtttgtgc agctgcttag ggtagaactg acgagatttc ggccagaggc
      901 gggagccact gccaccggaa agtaccacag gtacaactac agtcatgaac gatccttaag
      961 ccaaaaattg gtcgggcatt atatagaggt tgccactaca agccaaattt gctggctact
     1021 gcacactcgt gggagcctat attagcgccg attataaaaa acatagcccc tgagcctgtg
     1081 caggggcctg taagacacgt cacaagggtg tgattcgctt gggaagtcgg aaagtggatc
     1141 ctggatggca caatcggtaa atatcgacaa aggcgcttag cttaatgaca ttccccttga
     1201 aggggtagct ggagtggttg cggggtgcct gttggatgtg atggggagca gttttttcct
     1261 gtgtagtgag attgttaaca ataaaaccta gtggcagcct accagccctc tccttcggga
     1321 caccactccg caggagtggg aattttaggg cctgcttgtt tctggtaatt ccttaactta
     1381 ataacttgcc tcgcggcctt tacctcgaaa ggtggcggca aggcctatag cgggccaggc
     1441 tcaaaacagc ctttttacgg ccagagataa aagccttacg ggccaggcac aacaaagctc
     1501 atccaaggca aatacaaaaa cccacatgac cagaaatata caagcccctt aaaagccaga
     1561 aaatccactc accaaccgga agcaaacaag ctccttacac gccagatccc caaaaacctc
     1621 aggcgagcca aataaaaaag ccccttgcgg ggcttggtgt gtggccttgg ctcgggtgag
     1681 ctttggctgt tgagccaacg ggctgcggct acataggcgg ttgatccgcg ggtgctgggc
     1741 cgttggcggc tgggttgaag ttgctgagga tggcggtacg ggcggcgctg ttgtagacga
     1801 tgtgttgttg cttcacaccg gcagcgagca ggtggccgtg gcgcactagg ggggtgttgt
     1861 ccagggcggc ttcttcggta acaaataggc cgctggcttc atggtcccag tcgctattgg
     1921 cgattaactc gttcggcagg tttaccgcca gcagggtttt accggctttg acgatttttt
     1981 gcaccggcag atcagctaat accttaggca cttggttagc ttgagactgc agcacggggc
     2041 gcaagcttaa ggtgccgttg aggctgccgt cgctggagat aaagtagccc tggatattca
     2101 ccatggcacc gctggcctcg tggctactgg cgatccacac cggtactgct ttgcccgtgg
     2161 caaattcatc aggacttgca accacgtcat caaggctagt ggcgggtacg ggggtattgg
     2221 gttggccttc gccagcgggc tgccaggctt tggcgactat gttgtcgcat tctaagtggc
     2281 tggcaaacca ggggtcgaag ccttcattgc agtagaactc agcgctcagg gcaacctggg
     2341 tcaactcggc cacccaggct ttggcgcctt gggggaactc ggcgcttggg ggtatggctt
     2401 tggcgatttc cggtgcgagc agttggctaa ggttttggcc gccgaggtca aactcgtgca
     2461 gttgcaggtt cataccgggc actgccaggg cttggcctgc cacgcttggc accaggtggg
     2521 caccctcgcc ttcggggccg aagtccgtaa gcatgatcag gtcatcgctc agcaccaagc
     2581 cctgctcggt aactagcacg gtagccttgt ggtggctggc cgagttgtcc tgccaggttt
     2641 tgctggccgg gtcgtagtgg aaggtactct ccactgcttg cggggcgatc aggccatagg
     2701 tgaagtcact gctgtagccc tgctcgctga acaggttctc cacatcaaac cagttgaggc
     2761 tgtcgccgct gagggcggcg gcttcgttga cgggtttggc tttctgtgcc gttaccaaag
     2821 cctcttgagt gcattggccg cccactttca gcacgaagct taggttgccc tgagcatcaa
     2881 agcttgcgtt gctggcgaag acttgcggct gggtagcggt gagcaccagc ttgccatcgg
     2941 cctgcactac acccactagg aaggccgcat cccgggggtg aaagtc
//
LOCUS       DAOQGS010000520         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19852_length_2983_cov_4.310109, whole genome shotgun sequence.
ACCESSION   DAOQGS010000520 DAOQGS010000000
VERSION     DAOQGS010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19852_length_2983_cov_4.310109"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKZ52_15605"
     CDS             <1..335
                     /locus_tag="PKZ52_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HNG61247.1"
                     /translation="TLDRLVLLTDDQRELSYYSLNFGYNLLPGEHYVGRWAFNTNFYI
                     VAGAGSTLFADEEYFTYNFGGGLRFFATDWLALHVDFRNYVFTHSLFGEDKKVQNLES
                     SLGATLFF"
     gene            375..863
                     /locus_tag="PKZ52_15610"
     CDS             375..863
                     /locus_tag="PKZ52_15610"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNG61248.1"
                     /translation="MKNLFVTLAAAFLAASVNAADVAKGPAPDFTLKSTTGQNVKLSD
                     LKGKVVLINMWASWCGPCRQEMPILDKLAKDNKDAGLVVLGVNQDEEASERDAFLKDN
                     PVSFQILDDSKHAVAGLLKSKTQPSSFFVDRAGNLVHAHEGFKAGDDKVYAATVKELL
                     AK"
     gene            863..1552
                     /locus_tag="PKZ52_15615"
     CDS             863..1552
                     /locus_tag="PKZ52_15615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNG61249.1"
                     /translation="MPNLAAPAIGFGCGRFSLPTAKAHRRSGQSPQAKQSPQAKLARP
                     FAAPWGHQGKGLILCCLALLASHSHGAATLGNALPTSGSASQVAAKLPPAPDFTLQSI
                     RGETVKLSHFKGQVVLVNFWASWCPPCRAEMPVLDNLAQTHKTAGLVVLGLNVDVDNE
                     SRDEYLKEHPVSFPILDDSKWSTAKLYIAPSQPVTFFVDRDGNLAHVHQGYKSGDDLV
                     YATKVIELLAK"
     gene            1554..1778
                     /locus_tag="PKZ52_15620"
     CDS             1554..1778
                     /locus_tag="PKZ52_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNG61250.1"
                     /translation="MRNLAAIVVLITSCGCSHLQPEPWVKAYERGHLADPVMKLERSA
                     VAGKYMNHVYEAREAARGADGSAGGGCGCN"
     gene            1765..>2983
                     /locus_tag="PKZ52_15625"
     CDS             1765..>2983
                     /locus_tag="PKZ52_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNG61251.1"
                     /translation="MAAIKVLAAALLVFLSLNLQAAILPEESADLLYHSYDGGGVTVN
                     GPSVLVRKNFKDTASVYANYYTDMVSSASIDVMTAGSKYSEQRTEYSTGVDYLNDTTI
                     YSLGFTNSSENDYEANTINFGISQDFFGDLTTVTMGYTRGDDTVMMNGDESDPVQFEA
                     SQKHQRFSLGLSQVITKNWLATANVESVIDNGYLNNPYRSVRYLDDGKVSYQTERYPQ
                     THNSDAIALRNIYYLPFRSSIKGEVKFFQDSWGVDATTYELKYVHPWKRTWIFEAKLR
                     AYSQSQADFYADIFPHKDFQTFLARDKELSQFSSTNFGLGVTYKLPKPPLAFLNNCTV
                     NFYWDALQFKYDNFHNALLSKASADGKEAAYKPGEEPQPITPALPLRYADFVIDLRRR
                     RILAVREDHRDAGR"
BASE COUNT          739 a          842 c          719 g          683 t
ORIGIN      
        1 aaaccctcga ccgcttagtg ctgctcaccg atgaccaacg ggagttaagt tattattcac
       61 tgaacttcgg ttacaacctg ttgccaggtg agcactatgt gggccggtgg gcatttaaca
      121 ccaatttcta tatagtcgcg ggcgccggaa gtaccctatt tgccgatgaa gagtacttta
      181 cgtacaactt cggtggaggg ctgcgatttt ttgccacgga ctggttggcg ctgcatgttg
      241 atttccgcaa ttacgtcttt acccactcct tatttggcga agacaaaaaa gtgcagaacc
      301 tcgaatcaag cctgggtgcc accttgtttt tttaactctt tatgtattgt ttacactgtc
      361 cttggaggaa ttaaatgaaa aaccttttcg taactcttgc cgctgctttt cttgctgcga
      421 gtgtcaacgc cgctgacgta gccaaaggcc cagcgccaga cttcacctta aaatcaacca
      481 ctggccaaaa cgtcaaactc tccgacctca aaggcaaagt ggtgttgatc aacatgtggg
      541 cttcctggtg tggcccctgc cgtcaagaaa tgccgattct cgataaactg gctaaagaca
      601 acaaagacgc gggccttgtg gtactgggcg ttaaccaaga tgaagaagcg tccgagcgcg
      661 atgccttctt aaaagacaac ccagtttctt tccaaatttt ggatgacagc aagcatgcag
      721 ttgcaggcct gctgaaaagc aaaacccagc catccagctt ttttgtggat cgcgctggca
      781 acctagtcca cgcccacgaa ggctttaaag cgggcgacga caaggtatac gctgccactg
      841 ttaaagagtt gttagctaag taatgcccaa cctggccgct cccgctatcg gctttggctg
      901 cggcaggttt agcctgccaa cagccaaagc ccacaggcgg agcggccaga gcccccaggc
      961 taaacagagc ccccaggcta aactagcccg cccttttgct gcgccttggg gccatcaggg
     1021 caagggcctc atactgtgct gtctggctct gctagccagc catagccatg gggccgccac
     1081 cctgggtaac gcgctcccca cctcggggtc agccagccaa gtcgccgcta aactcccccc
     1141 agctcctgac ttcaccctac agtccatacg gggcgaaacc gtcaagctgt cccactttaa
     1201 ggggcaagtt gtgctggtga atttctgggc cagctggtgc ccaccctgcc gagcagaaat
     1261 gccggtgtta gacaaccttg ctcaaaccca caaaacagct ggcctcgtgg ttttggggtt
     1321 aaatgtggac gtggataacg aatcccgcga cgaatacctc aaagaacatc ccgtctcttt
     1381 cccaattttg gatgacagca aatggagcac ggctaaactg tacatagccc cttcacaacc
     1441 ggttaccttc tttgtcgatc gagacggcaa cctggcccat gtgcatcaag gttataaatc
     1501 cggtgacgac cttgtgtacg ccaccaaagt tatagaatta ctggccaagt gatatgcgca
     1561 acctagctgc gatagtagtg ttaataactt cctgtggctg cagccaccta caacccgagc
     1621 cctgggtaaa agcttacgag cggggccact tggctgaccc ggtaatgaaa ctggagcgca
     1681 gcgcggtggc cggtaaatac atgaaccacg tgtacgaggc ccgcgaagcc gcacgcggcg
     1741 ccgacggcag tgccggaggt ggctgtggct gcaattaaag ttctagccgc tgcgctgctg
     1801 gtttttttga gcctcaacct acaagcagcc attctccccg aggaaagcgc tgacctgctt
     1861 taccactcct acgatggcgg cggcgtaacc gttaacggcc catcggtgtt agtgcgcaag
     1921 aattttaaag ataccgcgtc ggtgtacgct aactactaca ccgacatggt ctccagcgcc
     1981 tccatcgacg ttatgactgc aggcagtaaa tactccgagc agcgcaccga atattccacc
     2041 ggcgtggact acctcaacga caccaccatc tacagcttgg gttttactaa ttccagcgaa
     2101 aacgactacg aagccaacac aatcaatttc ggtatttcac aggatttttt tggggacctc
     2161 accactgtca ccatgggcta tacccggggc gatgacacgg tgatgatgaa tggcgatgag
     2221 agcgacccgg tacaatttga agccagccaa aaacaccagc gctttagcct aggcttaagc
     2281 caggtgatta ccaaaaattg gttggccacc gccaatgttg aatcggtcat tgataacggc
     2341 tatttaaata acccttaccg ctccgtgcgt taccttgatg acggtaaggt cagctatcaa
     2401 accgaacgct acccgcaaac ccacaatagc gatgccattg cactgcgcaa tatttattac
     2461 ctgccgtttc gctcatcaat aaaaggcgaa gtaaaatttt tccaagacag ttggggggta
     2521 gatgccacca cctacgaact aaaatacgtg catccttgga agcgcacctg gatttttgaa
     2581 gctaaattgc gcgcctattc tcaatcacaa gcggattttt acgcagatat tttcccccat
     2641 aaagatttcc aaacctttct cgcccgcgat aaagaactca gccaattcag ttccaccaat
     2701 tttggtttag gtgttaccta caaactcccc aaaccgccct tggccttttt aaacaattgc
     2761 accgtgaatt tttattggga tgcactgcaa tttaagtatg ataactttca caacgcttta
     2821 ttgagtaaag cgtctgccga cggcaaagaa gcagcgtaca agcccggcga ggagccgcag
     2881 cccatcacgc cggcgttgcc cctgcgctat gccgacttcg tcatcgacct gcggcggcgg
     2941 cgcatcctgg ccgtccgcga ggaccatcgc gatgcgggcc gcg
//
LOCUS       DAOQGS010000521         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19877_length_2980_cov_6.684103, whole genome shotgun sequence.
ACCESSION   DAOQGS010000521 DAOQGS010000000
VERSION     DAOQGS010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19877_length_2980_cov_6.684103"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..2107
                     /gene="fusA"
                     /locus_tag="PKZ52_15630"
     CDS             8..2107
                     /gene="fusA"
                     /locus_tag="PKZ52_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNG61252.1"
                     /translation="MARKTPIARYRNIGICAHVDAGKTTTTERVLFYTGVSHKIGEVH
                     DGAATMDWMAQEQERGITITSAATTCFWKGMGQQFEDHRINIIDTPGHVDFTIEVERS
                     LRVLDGAVVVLCGTSGVQPQTETVWRQANKYHVPRLVFVNKMDRTGADFRRVVAQLKN
                     RLGANPVPLQMTIGAEEHFKGVVDLIKMKAIIWNEQDQGMTFTYGDIPADIQDECEDM
                     RNFLVESAAEANDEYMDKYLGGEMLTEDEIKAGIRARTLAGEIIPVIGGSAFKNKGVQ
                     AMLDAVIEYLPAPTEVKPIEGILENGQTAVRHADDAAPFAALAFKIATDPFVGTLTFF
                     RVYSGTLNSGDAVFNPVKGRKERIGRMVQMHANQRDEIKEVLAGDIAAGIGLKDVTTG
                     DTLCAIESVITLERMEFPEPVISLAVEPRTQADQEKMGVALGKLAQEDPSFRVKTDEE
                     TGQIIISGMGELHLDIIVDRMRREFNVEANVGKPQVAYREAIRNTCEIEGKFVRQSGG
                     RGQYGHCWIRFEPGEKEGLEFVNEVVGGVVPKEFIPAIGKGIEEQMKNGVLAGYPLLG
                     LKATVYDGSYHDVDSSEMAFKIAASMATKKLSQHGGAVLLEPVMKVEVITPEENMGDV
                     VGDLNRRRGLILGMEESSMGKIINAEVPLAEMFGYATALRSATQGRATYSMEFAQYSE
                     APANIVKEIIARNSTGG"
     gene            2156..>2980
                     /locus_tag="PKZ52_15635"
     CDS             2156..>2980
                     /locus_tag="PKZ52_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNG61253.1"
                     /translation="MAKAKFERNKPHVNVGTIGHVDHGKTTLTAALTRVCAETWGGQA
                     VAFDGIDKAPEEKARGITISTSHVEYDSADRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCGTTDGPMPQTREHILLSRQVGVPYIVVFLNKADLLADDCGGVGSDEYNEMLELV
                     EMEIRDLLNTYEFPGDDTPIIAGSALMALEGKDDNELGVSAVRKLVQALDSYIPQPER
                     AIDKPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEVEIVGIRATTKTTCTGVEMF"
BASE COUNT          739 a          766 c          816 g          659 t
ORIGIN      
        1 gttcaccgtg gcacgcaaaa ctcccatcgc acgctaccgc aatattggta tctgcgccca
       61 cgtagacgca ggtaaaacca ctactaccga acgcgttcta ttttatacgg gtgtgtccca
      121 caaaatcggt gaggttcacg acggtgctgc caccatggat tggatggccc aagaacaaga
      181 gcgtggtatc actatcacct ccgccgccac tacctgcttc tggaagggga tggggcaaca
      241 attcgaagac catcgcatca atattatcga tactcctggc cacgttgact tcactattga
      301 agttgaacgt tctctgcgag tgctcgacgg tgcggtagta gtgttatgcg gcacctcagg
      361 tgtgcaaccg cagaccgaaa ctgtatggcg ccaagccaac aaataccatg tgccgcgcct
      421 ggtgttcgtc aacaagatgg atcgcaccgg ggcggacttt cgccgtgttg ttgcccagct
      481 gaaaaatcgc ttgggtgcca acccggtgcc attgcaaatg accatcggcg ccgaagaaca
      541 ctttaagggc gtggttgatc tgatcaaaat gaaggccatc atctggaatg aacaagacca
      601 gggcatgacc ttcacctatg gtgatattcc cgccgatata caggacgagt gcgaagacat
      661 gcgcaacttc ctggtggaat cagcggcgga agccaatgac gaatatatgg ataagtacct
      721 aggtggcgag atgctcaccg aggacgaaat caaggcgggc attcgcgccc gcaccctggc
      781 gggtgaaatt atccccgtta tcggtggctc ggcgtttaaa aataaaggcg tgcaggccat
      841 gctggatgct gtcatcgagt acttgccggc acccaccgaa gttaagccga tcgagggtat
      901 cttagagaac ggccaaaccg ccgtgcgcca tgcggacgac gcggcaccct ttgctgcgct
      961 ggcctttaaa attgccaccg acccctttgt gggcaccttg acctttttcc gcgtctattc
     1021 cggcacactt aattctggcg atgcggtgtt caatccggtt aagggtcgca aagagcgcat
     1081 tggtcggatg gtgcagatgc acgccaacca gcgggacgaa attaaagagg tgttggctgg
     1141 cgacatagcc gctggcatcg gcctaaaaga tgtcaccact ggagatacgc tttgcgctat
     1201 tgaaagcgtc attacccttg agcgcatgga attccccgag ccggtcattt ccctggcggt
     1261 ggagccgcga acccaggccg accaggaaaa aatgggagtg gcattgggta aattggccca
     1321 agaagaccct tctttccggg tgaaaaccga cgaagaaact ggccagatca ttatctcggg
     1381 catgggtgag ttgcatctgg atatcattgt tgatcggatg cggcgggaat ttaacgtcga
     1441 agccaatgtg ggcaagcctc aggtggccta tcgagaagct attcgcaaca cctgcgaaat
     1501 tgaaggtaaa tttgttcgcc agtccggcgg gcgcggccag tacggccact gctggattcg
     1561 ttttgagcca ggtgaaaaag aaggcttaga atttgtcaac gaagttgttg gcggcgtggt
     1621 gcccaaggag tttattccgg ccatcggcaa gggcattgaa gagcaaatga aaaacggcgt
     1681 gctggcgggt tatcctctgc tgggcttaaa ggccacggtc tatgatggct cttaccatga
     1741 tgtggactca tctgaaatgg cctttaaaat tgctgcttct atggccacca aaaagctttc
     1801 ccagcacggc ggtgctgtat tacttgagcc tgtcatgaag gttgaggtga tcacgccaga
     1861 ggaaaacatg ggtgatgtgg tgggcgatct caatcgccgc cgcggcctga tattgggtat
     1921 ggaagagtct tccatgggta agatcatcaa tgccgaggtc cccttggccg agatgtttgg
     1981 ctacgcgacc gcactgcgtt cggccaccca ggggcgcgcc acctattcga tggaatttgc
     2041 ccagtacagt gaagctccgg ccaacattgt caaagaaatt atcgccagaa actcaactgg
     2101 cggctaaagt acgcaaactt ttttaactta aaaaccttat taagaggaaa caacagtggc
     2161 aaaggctaaa tttgaacgta acaaacccca cgtaaacgtc ggcaccattg gccacgtaga
     2221 ccacggtaaa acgacgctga cagcagctct tactcgcgtc tgcgcagaaa cctggggtgg
     2281 ccaagcggtt gcttttgacg gcatcgacaa agcgcctgaa gaaaaagctc gtggtatcac
     2341 catctctacc tcacacgtgg aatacgattc agctgatcgt cactacgccc acgtagactg
     2401 cccaggccac gccgactatg tgaaaaacat gatcaccggt gctgcccaga tggacggcgc
     2461 gattctcgta tgcggcacca ccgacggccc tatgccgcaa acccgcgagc acatcctgtt
     2521 gtctcgccaa gtaggcgtac cttacatcgt ggtgttcttg aacaaagctg atctgttggc
     2581 tgacgactgt ggcggtgtag gttctgacga atacaacgaa atgttggagc tggttgagat
     2641 ggaaattcgg gacctattga acacctacga attcccaggt gacgacactc caatcattgc
     2701 aggttctgct ctgatggctc tggaaggtaa agacgacaac gaactgggcg tatccgctgt
     2761 tcgtaaactg gtacaggctc tggattctta catcccacaa cctgagcgcg ccatcgacaa
     2821 gcctttcttg atgccaatcg aagacgtatt ctccatctct ggtcgcggta ctgtagtaac
     2881 tggccgtgta gagcgtggca ttgtaaaagt gggtgaagaa gttgaaatcg tcggcattcg
     2941 cgccaccacc aaaactactt gtactggtgt ggaaatgttc
//
LOCUS       DAOQGS010000522         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19893_length_2979_cov_3.967168, whole genome shotgun sequence.
ACCESSION   DAOQGS010000522 DAOQGS010000000
VERSION     DAOQGS010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19893_length_2979_cov_3.967168"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKZ52_15640"
     CDS             complement(<1..429)
                     /locus_tag="PKZ52_15640"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG61254.1"
                     /translation="MLSLTLAPPHNPLNHPAPKLIRQADQLWQLTFNAMASPCEVLVD
                     APQLAVAQLVPLCEQLVSETWRIEHKYSRYQAGNIIHRLNQAQGQPVLLDHETQQLIA
                     FADTCFQLSGGLFDITSGILRRVWRFKPDQPVPRQDQIDAL"
     gene            complement(673..1986)
                     /locus_tag="PKZ52_15645"
     CDS             complement(673..1986)
                     /locus_tag="PKZ52_15645"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNG61255.1"
                     /translation="MQLTKSGTIAQALAGATCALLGLNTQAGEAGSWDFDTAVLYYGE
                     DNGRVQALEPVVAATRYFADERQLGIKLTLDSLTGASPNGATPSDQPQTFTRPSGKGA
                     YTIAPGEAPLDDTFKDTRGALNLNWSSPINSDWRYSSGFNVSAEHDYFSAGLNGSISR
                     YLNEKNTELTLGLAAASDSVKPEGGVPKGLVRVPKAGVADFWPQFDSTRQGDSDTKTL
                     TDVLLGVTQVINKRTIMQFNYSLSTSSGYLTDPYKLLSVIDATSGKNALDGTGAPIYV
                     YEQRPDSRTKHAFFWQTKYMLERGDVIDGSYRFMTDDWGITSHTIDLKYHWQLDHAYL
                     EPHLRYYTQSQADFYHRYALNTDYNSSTQTLNLSDASADYRLGDLTGTTVGLKYARQF
                     NQQEFSVRAEYFLQSNSGDKGVGALANQELYPDTNAVLFTLGYSF"
     gene            complement(1977..2195)
                     /locus_tag="PKZ52_15650"
     CDS             complement(1977..2195)
                     /locus_tag="PKZ52_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNG61256.1"
                     /translation="MRITLLCTALASLMLTGCSQLGVKSYERGLLAKESMLLTSDPIE
                     AGLDEHIYFSKEASSGGRGFGGGGCGCN"
     gene            complement(2604..>2979)
                     /locus_tag="PKZ52_15655"
     CDS             complement(2604..>2979)
                     /locus_tag="PKZ52_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNG61257.1"
                     /translation="VIAHEVGHHVQYLLGINAQVEKRSAGLSAVERNRLSVKQELQAD
                     CFAGIWGFYAKQKGNFLETGDLEEALNAASAIGDDHLQAAAGRPVRPESFTHGSAKER
                     VYWFNKGFNEGSVASCNTFTST"
BASE COUNT          735 a          712 c          816 g          716 t
ORIGIN      
        1 cagggcgtca atttgatctt gcctggggac gggttggtcg ggtttaaagc gccacacccg
       61 ccgcaaaatt cccgaggtaa tatcaaataa accgccgctt aattgaaagc aggtatcggc
      121 aaaggcgatt aattgctggg tttcgtgatc caacaacacc ggctggccct gtgcctggtt
      181 gagccggtgg atgatattac ccgcctgata acgactgtat ttatgctcaa tgcgccaggt
      241 ttcgctcacc agctgttcac atagggggac taattgcgcc acggctaatt gcggcgcatc
      301 cactaagact tcacaggggc tggccatggc gttaaaggtt aattgccaga gttgatcggc
      361 ttggcgaata agcttagggg ctgggtggtt gagcggatta tggggcggag cgagagttag
      421 gctaagcatg gggccccgtg aaattcgcat gaaatagaaa aatgaagccc agtttaaggc
      481 ctgggcggcc gctcgcgaaa acaggtaaca aatgcccggc cagcaggagc aaataataac
      541 tgctcaaatc gttgcgcgta aatttgcgta cgaaaacgaa cagctgcgtg gcactattta
      601 aaaaactgac actaaaaaaa ttgacccggc atattggccg ggtcaatttg aacctttgca
      661 gctgttgccg gattaaaacg aataacccag ggtaaacagt accgcattgg tgtcagggta
      721 taactcctga ttagccaatg cacccacgcc tttatcgccg gaattacttt gcaggaaata
      781 ctccgcgcgc acggagaatt cctgctgatt aaattgccgt gcgtatttaa ggccgacggt
      841 ggtgccggtt aaatcaccca ggcggtaatc cgcactggcg tcacttaaat taagcgtttg
      901 ggtgctgctg ttgtaatccg tattcaacgc gtagcggtgg taaaaatccg cttgcgattg
      961 ggtgtaataa cgcaaatggg gctcaaggta cgcgtgatcc aactgccagt ggtattttaa
     1021 gtcgatggtg tgggaggtaa tgccccagtc atcggtcata aaacggtagg agccgtcgat
     1081 cacatcgccg cgctcgagca tgtatttggt ctgccagaaa aaagcgtgtt tggtgcgcga
     1141 atccggccgc tgttcgtaca cataaatagg cgcaccagtg ccatccagcg catttttacc
     1201 gctggtcgcg tcgataacgc tgagcaattt atacgggtcc gtcagatagc cgctgctagt
     1261 gcttaaggag tagttaaact gcatgatggt gcgcttgtta ataacttgcg tcacccccaa
     1321 aagcacatcg gtaagggttt tagtgtcgct gtcgccctgg cgggtgctat caaattgggg
     1381 ccagaagtca gcaacacccg ctttgggaac ccgcactaaa cccttcggca cgcccccttc
     1441 gggttttacc gaatcgctgg cggccgcaag gcccagggta agctcggtat ttttctcgtt
     1501 taaataacgg ctaatcgagc cattgaggcc agcgctaaag tagtcgtgct cggcggagac
     1561 attaaacccg ctgctgtagc gccagtcgct gttaataggg gacgaccaat tgagattgag
     1621 cgcaccgcgg gtatctttaa aggtgtcatc caggggggct tcaccggggg cgatggtgta
     1681 ggcgcccttg ccgctggggc gggtaaaggt ttgtggttga tccgaggggg tggcgccgtt
     1741 gggggatgcg ccggtgaggg agtcgagggt caatttgata cccaattggc gctcatcggc
     1801 aaaataacgg gtggcggcta ccaccggctc cagggcttgc acccggccgt tgtcttcacc
     1861 gtaatacagc acggcggtat caaaatccca gctgcccgct tcgcccgctt gagtattcag
     1921 gcccaacagg gcacaggtgg cgcccgcgag ggcttgggca atcgtgcccg atttagttag
     1981 ttgcatccgc agccacctcc gccaaaaccg cggccgccgc tgctggcttc tttactgaaa
     2041 taaatatgct catccaaacc cgcttcaatg ggatctgatg tgagcaacat ggattctttg
     2101 gccaacaggc cccgctcata ggacttaacg cccagttgac tgcagccggt cagcataagg
     2161 ctggccaggg cagtgcataa aagtgtgatg cgcatgaaat aaccgatgta tggtgggttt
     2221 agacaagcct aaatgtaaaa ccttaagtgt ggattaaaca cccaacagaa cagattttat
     2281 tttgccgatt cagcaattta atgagtgact ttttatggaa tgcaaataat ttccattcgc
     2341 gcagagtttg acgggctttt aagatcggct taataacttt gcggattagc ctgggggcgt
     2401 ctgaaaattt cctcactcaa cctgttggta ttgaaaatgg caaaaagcgg aggtggcacc
     2461 ggatggataa ccattaatgc agtgcgctag cccaaattta acgccgccat agtgagaaag
     2521 gaaatatttt aaaaagctgc aaagtaagta ttaatggtaa attcgagcaa tcttgcgact
     2581 gtattgcttg cgaaaattta atattacgtg cttgtaaatg tattgcagct tgccacggag
     2641 ccttcattaa agcctttatt aaaccaataa acgcgctctt ttgcgctgcc gtgggtgaag
     2701 gattctgggc gcacagggcg gcctgcggct gcttgtaaat gatcatcgcc gatggcgctg
     2761 gcggcgttga gggcctcttc taaatcgccc gtttccaaaa aattaccttt ttgtttcgcg
     2821 taaaatcccc aaatgcccgc aaaacaatcg gcctgtaatt cttgtttaac tgaaaggcga
     2881 ttgcgctcaa ccgcggataa tccggcggag cgtttttcaa cttgggcgtt aatacctaat
     2941 agatattgca cgtggtggcc cacctcgtgg gcaatgaca
//
LOCUS       DAOQGS010000523         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_19963_length_2974_cov_3.715313, whole genome shotgun sequence.
ACCESSION   DAOQGS010000523 DAOQGS010000000
VERSION     DAOQGS010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19963_length_2974_cov_3.715313"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PKZ52_15660"
     CDS             <1..1638
                     /locus_tag="PKZ52_15660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNG61258.1"
                     /translation="PAAKVEPVTAKAEPASDEISDAEFESLLDQLHGTGKGPGRASAP
                     SVASAPSAPAATAAPAADIISDDEFEHLLDQLHGRGKGPTTTEKVASAPPTPPPAAAR
                     VEPAAPEPSRSAGAEIPPAAVSPPVAPVIRAAPPARAAEADEEARNRDSATHAVATET
                     TVRVDTQRLDDIMNMVGELVLVRNRLVRLGLDSSDEALAKAVANLDVVTADLQTAVMK
                     TRMQPIKKVFGRFPRVVRDLARNLKKEINLDLVGEETDLDKNLVEALADPLVHLVRNS
                     VDHGIELPAVREAAGKPRTGKIVLSAEQEGDHILLSITDDGGGMDPQKLREKAIEKGI
                     VDPDAAARLSDMEAFNLIFAPGFSTKSEISDISGRGVGMDVVKTKISQLNGSIEIFSK
                     IGEGTRIAIKVPLTLAIMPTLMIMLGRQTFALPLVSVNEIFHMDLTKAHIVDGQECVT
                     VRAKAIPIFHLKRWLVKGLTSFERPKQGHVVIVNVGTRRVGFVVDQLIGQEEVVIKPL
                     GKMLQGTPGMAGATITGDGTIALILDVPSMLKRYVGG"
     gene            1805..>2974
                     /locus_tag="PKZ52_15665"
     CDS             1805..>2974
                     /locus_tag="PKZ52_15665"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNG61259.1"
                     /translation="MVYNVLIVDDSNFFQVRLKEIINEHPELKVVGIAANGREAVDMA
                     RDLKPDIISMDYEMPYMDGVSAIKLILSERPVPIVMFSSLTYEGARTTLDALAAGAVD
                     FIPKNFAEVSRNSAELKRKLHETLLTFAKKSNVRVPAPTHTSSNPSADASRPVPAARP
                     RDTQGRQPATPTPSVTPGAAPAYLNPAAHKPLETTSVDSRPNDSRPVESRPAAKPLRG
                     RVRLVIIGSSTGGPMALTEVLMQLSASFSVPIVVVQHMPPNFTKALAERLDRQCQVRV
                     KEAATGDQLVPGHVYIAPGGHQLIFERGRGVIKILPGDERMNYKPSVDITFASAANEF
                     GGAVLGVVLTGMGADGCEGARLLKDKTATIWGQDEASCVVYGMPKAVASAGLTDEV"
BASE COUNT          691 a          764 c          864 g          655 t
ORIGIN      
        1 cctgcggcta aagttgagcc tgtaaccgcg aaggctgagc ctgcctctga tgaaatatcc
       61 gacgctgaat ttgaaagcct gctcgatcaa ttgcacggca caggtaaagg gcctggccgg
      121 gcatctgcac cctccgtagc ctctgcaccc agtgcgcctg ctgcgaccgc tgcgcctgcc
      181 gccgatatta tttccgatga cgagtttgag catttgctcg accaattgca cggtcgcggc
      241 aagggcccta caacgactga gaaagtggcc agtgcaccgc caacgccgcc gcctgccgcc
      301 gcccgcgtag aacctgccgc cccagaaccc agccgcagcg ccggggcgga gattcccccc
      361 gccgctgttt cgccgccggt tgcgccggta atccgcgctg cgccacctgc gcgcgccgcc
      421 gaggccgacg aagaggcccg caatcgagat agcgcaaccc atgccgtggc caccgaaacc
      481 accgtgcggg tggatactca gcgcctggac gacatcatga atatggtggg tgagctggta
      541 ttggtgcgca accgcctggt gcgtttgggt ttggattcca gcgacgaagc cctcgccaaa
      601 gcggttgcta atttggatgt ggtaactgcc gatttacaaa cggcggtgat gaaaacccgc
      661 atgcaaccga tcaaaaaggt gtttggtcgt tttccccgtg tggtgcgcga tttagcccgc
      721 aatttgaaaa aagaaattaa tttagattta gtgggtgaag agacggacct ggataaaaac
      781 ctggtagagg cgctcgcgga cccattggtg cacttggtgc gcaattccgt tgaccacggc
      841 attgaattac ccgccgtgcg cgaagccgcg ggtaaacccc gcaccggtaa aattgtgctc
      901 tccgcagagc aggaaggcga tcatattttg ctgtctatta ccgacgacgg cggcggtatg
      961 gacccgcaaa aattgcggga aaaagccatt gaaaaaggca tagtggaccc ggacgccgcc
     1021 gcgcgtttgt cggatatgga agcgtttaat ttaatttttg ccccaggttt ttccaccaag
     1081 tcagaaattt ccgatatttc tggccgcggt gtgggcatgg acgtggtgaa aacaaaaatc
     1141 agccaattaa atggctccat tgaaattttc tccaaaatag gcgagggcac acgcattgcg
     1201 ataaaagtgc cgctcaccct ggcaattatg cccaccctga tgattatgct gggcagacaa
     1261 acctttgctt tacccttggt gagtgtgaac gaaattttcc atatggattt aaccaaagcg
     1321 cacatagtgg atggacagga atgtgtgacg gtgcgcgcca aagcgattcc gatttttcat
     1381 ttaaagcgct ggttggtaaa aggcttaacc tcgtttgagc gccccaaaca aggccatgtg
     1441 gtaattgtga atgtgggtac ccggcgcgtg gggtttgtgg tggatcagtt aattggccag
     1501 gaggaagtgg tgattaaacc cctgggtaaa atgttgcaag gtacaccggg tatggcgggg
     1561 gccactatta cgggcgatgg caccattgct ttgatattag atgtgccgag catgttaaaa
     1621 cgctatgtgg gcggttgatg cgcccgcatt aggttcttca cagctaataa gtgcagctat
     1681 aaaggcaaat tattggtgtg agggagccgc agtagcaagg gagagttgtg ataattaaaa
     1741 cgagattgct gcaggcggct attgagcggt ttgggtctca acgtaaaaac accgaggagt
     1801 gacagtggtt tataacgtgc tcatagtcga tgattcgaat ttctttcagg tgcgcttgaa
     1861 ggaaataatt aatgagcacc cggaattaaa agtggtaggc atagccgcca atggccggga
     1921 agcggtggat atggcgcggg atttaaagcc ggatattatt tctatggact atgaaatgcc
     1981 ctatatggat ggggtatccg ccattaaatt aattttaagt gaacgcccag tgccgattgt
     2041 gatgttttca tcgctcacct acgaaggcgc caggacaacc cttgatgccc tggcggcggg
     2101 ggcggtggat tttatcccga aaaattttgc ggaggtctcg cgcaactccg ccgagttaaa
     2161 acgtaaactg cacgaaaccc tattaacctt cgccaaaaaa tccaatgtgc gggtgcctgc
     2221 acctactcat acatcaagta atccgagcgc tgacgccagc aggcccgtac ccgccgcgag
     2281 gccgcgcgat acacaggggc gacagcctgc aacgcccaca ccttccgtaa cacccggtgc
     2341 cgcccctgct tatctcaatc ctgctgcgca caaaccgctc gagaccacgt ctgttgattc
     2401 taggcccaac gactccaggc cggttgaatc caggcccgca gctaaacccc tgcgggggcg
     2461 cgttcgcttg gtaattatcg gctcctcaac cggcgggccc atggccctaa cggaagtatt
     2521 gatgcagctg agtgcaagct tcagcgtacc cattgttgtt gtgcagcata tgccgccgaa
     2581 ctttaccaag gccttggcgg agcgattgga tcggcagtgc caggtgcggg tgaaagaggc
     2641 cgccacaggg gatcaattgg tgccgggcca tgtgtatata gcaccgggtg ggcaccagct
     2701 tatttttgag cgcggccgtg gagtcattaa gattctgccg ggcgatgagc gcatgaacta
     2761 caagccgtct gtggatatta cctttgcctc tgccgccaat gagtttggcg gtgctgtttt
     2821 gggggtggtt ttaaccggta tgggcgccga tggctgtgaa ggggctcgcc tactcaagga
     2881 caaaacggcg accatttggg gacaggacga agccagctgc gtggtgtacg gtatgcccaa
     2941 agcagttgcc agcgcagggc tcactgatga ggtc
//
LOCUS       DAOQGS010000524         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20002_length_2970_cov_4.239794, whole genome shotgun sequence.
ACCESSION   DAOQGS010000524 DAOQGS010000000
VERSION     DAOQGS010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20002_length_2970_cov_4.239794"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKZ52_15670"
     CDS             complement(<1..384)
                     /locus_tag="PKZ52_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HNG61260.1"
                     /translation="MELNSEYPVVVVGAGLVGASLALALRLRLPHLAILLIDQQGPQQ
                     PLAPGQMDPRVVALNLASQTLLDQLGVWGGLERLCAYEQMQVWDGEGSGAIEFAAREL
                     GQEQLGWIVENGHVLAELHRLIAAAP"
     gene            complement(374..1633)
                     /gene="ubiH"
                     /locus_tag="PKZ52_15675"
                     /gene_synonym="visB"
     CDS             complement(374..1633)
                     /gene="ubiH"
                     /locus_tag="PKZ52_15675"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HNG61261.1"
                     /translation="MAPQGIAIIGGGMVGASLALLLSRALPRCPIRLFESNPLLNGSE
                     EKSAAVQGSWDGRSTALAPTSVELFTALGLWPQIAPFATAIERIHVSDQGSWGLSQFT
                     RADNRQQPLGQVVENAGLHPVLHQALAQNPSIQVIPAAQVTALRPLSGGMQVISRADG
                     QEQQWPSQLVILADGADSPLRKALGVAAREVPYEQTALVANVRTSLPHGGCAFERFCP
                     DGPMALLPRGQSPQAHIAGLVWTFPQSQQELIAGLSDSELLERLQGAFGYRLGRFEAL
                     GQRQFYPLRLVVAKEQVRSHLVLQGNAAHFLHPVAGQGFNLSLRDGLRLAQVLSEGAA
                     LGEQLGELKQLQRYAQAAEADQNRTIALSDGFNRIFSRKERSLRWGRDIGMLLLESVP
                     AVRAAFIRQLSGRAQPVNLPRELTGGA"
     gene            complement(1633..2964)
                     /gene="pepP"
                     /locus_tag="PKZ52_15680"
     CDS             complement(1633..2964)
                     /gene="pepP"
                     /locus_tag="PKZ52_15680"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNG61262.1"
                     /translation="MSAITQSEFGARRRALMDAMETDSIAIIPSAQEKIRSRDTHYSF
                     RQDSDFHYLSGFNEPQAVLVLLPGRKQGEYVIFCRERNLEKEIWDGFRYGPEGACASF
                     GADDAYPIDDIDDILPGLIEGRKRVYYALGQEPSFDSRVMGWVNHIRSKVRSGAVPPG
                     EFIDLNHLLHNLRLFKSPAEQVVMGESCALAARAHVRAMQVCKPGMFEYQLEAEILHE
                     FAMGGAPNPAYNTIVGGGANACVLHYINNNCALKDGDLVLIDAGCEYQGYAADITRTF
                     PVGGRFSPEQRALYDLVLDAQLAAIEAVKPGNHWNQPHEAAVRVLTAGLLRLGLLQGE
                     LDELIERQAYRPFYMHRTGHWLGLDVHDVGDYKVGGDWRLFEPGMVLTVEPGLYVGKD
                     EPSVAKKWRGIGIRIEDDVLVTADGCRVLTSGVPKTSAAIEALMAGQGPLL"
BASE COUNT          553 a          948 c          929 g          540 t
ORIGIN      
        1 cggcgccgcc gcgatcagcc ggtgtaattc ggccagcaca tggccgttct cgacaatcca
       61 gcccagctgc tcctggccca gttcgcgggc ggcaaactcg atggcgccgc tgccctcgcc
      121 gtcccatacc tgcatctgct cataggcgca cagccgctct aagccgcccc acacccccag
      181 ttgatccaag agggtttggg aggcgaggtt aagggcgacc actcgcgggt ccatctgccc
      241 tggggccagg ggttgttggg ggccctgttg atcgattaat agaatggcca gatggggtag
      301 ccgcaggcgt agggccaggg cgaggctggc ccctacgagg cctgccccca ccacaaccac
      361 tgggtattca gaattaagct ccacccgtca gctcccgagg caagttaacc ggctgggcgc
      421 ggccggagag ttggcgaata aacgccgccc gcaccgcagg aacgctctcc agcagcagca
      481 tgcctatatc tcggccccag cgcagcgagc gctccttgcg ggagaaaata cggttaaagc
      541 cgtcactcaa agcaatagtg cggttttgat cggcctcggc ggcctgggca tagcgctgca
      601 gttgtttcag ctcccccaac tgctccccta aggctgcgcc ttcacttaac acctgggcta
      661 ggcgcaagcc atcccgcagg gagaggttaa acccctggcc tgccaccggg tgtaaaaagt
      721 gcgccgcatt gccctgcaaa accaagtggg agcgcacctg ttctttggcc accaccaggc
      781 gcagtgggta aaactgccgc tggcctaggg cttcaaaacg gcctaagcgg tagccgaagg
      841 cgccttgtaa acgctcgagc agctcgctat cgctaaggcc tgcaattaac tcctgctgac
      901 tctgcgggaa ggtccagact aggcctgcta tatgcgcctg gggggattgg ccgcggggca
      961 acagggccat ggggccgtcc gggcagaagc gctcaaaggc gcagccgccg tggggcaggc
     1021 tggtgcgcac attggccacc agggcggtct gctcataggg cacctcacgg gcggccaccc
     1081 ccagggcttt gcgcaggggg gagtcggcgc cgtcggcgag aatcaccagc tggctgggcc
     1141 actgctgctc ctgcccatcg gcccgcgaga ttacctgcat accgccactt aagggccgca
     1201 gggctgtcac ctgggcggcg ggaatcacct ggatgctggg gttttgtgcc agggcctgat
     1261 gcaacactgg gtgcaagcct gcgttttcca ccacctggcc caggggctgt tggcggttat
     1321 cggcgcgggt aaattggctt aggccccagc tgccctggtc ggagacatgg atgcgctcga
     1381 tggcggtggc aaagggggcg atctgcggcc acaggcccag ggcagtaaat aactccaccg
     1441 aggtgggggc cagcgccgtg gagcggccat cccaactgcc ttgcacggcg gcggattttt
     1501 cctcgctgcc gttaagcagg gggttggatt caaacaggcg aatggggcac ctgggcaggg
     1561 cgcgggagag cagcagggcg aggctcgccc ccaccatgcc gccgccgata atggcgatac
     1621 cttgcggcgc catcaaagca gcggcccttg gcccgccatc aaagcctcaa tggcggcgga
     1681 ggttttcggc acaccgctgg ttaacacccg gcagccatcc gccgtcacca atacatcgtc
     1741 ctcaatgcga atacctatgc cccgccactt tttcgcaacg ctcggctcgt ctttgcccac
     1801 atacaggcca ggctccacgg ttaacaccat gcccggctca aacaggcgcc agtcgccgcc
     1861 caccttgtaa tcccccacat cgtgcacatc caggcccagc cagtggccgg tgcggtgcat
     1921 gtaaaagggc cggtaggcct gccgttcaat cagctcgtcc agctcccctt gcaacagccc
     1981 caagcgcagc aggcctgcgg ttaacacccg caccgccgcc tcgtggggct gattccagtg
     2041 gttgccgggt tttaccgctt cgatggcggc tagctgggca tctaaaacta ggtcgtagag
     2101 ggcgcgctgt tcaggggaga aacgcccgcc caccggaaag gtgcgggtga tgtccgccgc
     2161 gtacccctgg tattcacaac ctgcgtcaat cagcactaaa tcgccgtctt taagggcgca
     2221 gttgttgttg atgtagtgca acacacaggc attggcgccg ccgcccacta tggtgttata
     2281 ggccgggttg ggagcgccgc ccatggcaaa ttcatggagg atttccgcct ccagctggta
     2341 ttcaaacata ccgggtttgc acacctgcat ggcgcgcaca tgggcgcggg cggccagggc
     2401 gcaggactct cccatcacca cctgctcggc ggggcttttg aacaggcgca ggttgtgcag
     2461 caggtggtta aggtcgataa actcccccgg cggcaccgcg ccgctgcgca ccttgctgcg
     2521 aatatggttc acccagccca tcacccggct gtcaaaactc ggctcctggc cgagggcgta
     2581 atagacccgt ttacggccct caatcaggcc cggcaatatg tcgtctatgt cgtcgatggg
     2641 gtaggcatca tcggcgccga agctggcgca ggccccttcc ggcccgtagc gaaacccatc
     2701 ccatatttct ttttcaaggt tgcgctcccg gcagaagatc acgtattccc cctgcttgcg
     2761 gccgggcagc aacaccagca ccgcttgggg ctcgttaaag ccgcttaagt aatgaaaatc
     2821 gctgtcttgg cggaagctgt agtgggtgtc ccgggagcgg attttctcct gggcggaggg
     2881 gatgatggcg atggagtcgg tctccatggc gtccatgagc gcgcgccgtc tggcgccaaa
     2941 ttcggactgg gttattgcgg acagggcagt
//
LOCUS       DAOQGS010000525         2966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20053_length_2966_cov_4.890416, whole genome shotgun sequence.
ACCESSION   DAOQGS010000525 DAOQGS010000000
VERSION     DAOQGS010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_2966_cov_4.890416"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PKZ52_15685"
     CDS             complement(<1..1096)
                     /locus_tag="PKZ52_15685"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG61263.1"
                     /translation="MPRVIGCALGGVLYLFGSAGWAGPEGGQIVAGQGVIEQVEKETR
                     IQQASDRLSIDWKSFDINADERVLFIQPNSTSIAFNRILSNKGSLIQGRIDANGQVVL
                     INPNGLIFTEGASVNVGGLVASALQMSDQDYLNGQFTLNALEGSEGRVINSGLLNAAT
                     GGTISLLGQSVENKGLISAQLGRVNVAAGKEAIVTFEPNGLVGVRVAEAILQKDLGVD
                     AAIINAGEVKAAGSKVLMTASTSKDIFSQAVNVGELSAAKSVVVNADGSFTLGAGADI
                     KNTGAINVGAAAQAGEAVLLGANITNQGSIQADSQTSHAGHIEITAQNINETKDQGTV
                     TAIAHGAGKGGDIKLLGEKVGLLDSARVDAS"
     gene            1287..1754
                     /locus_tag="PKZ52_15690"
     CDS             1287..1754
                     /locus_tag="PKZ52_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61264.1"
                     /translation="MKKRSIFSRIAILNEGREPFLNYLRNITPQAILLSFALLATYKS
                     TIKPQHHTGLMAFSLLFFSIFAAASYANITLFYKNIFKDSLKKNNASIIRLCRKHKFT
                     KYKQLIMIPWLTLKYKWVELIELLCTVVFLQSTIMIVFYLAGLQYSAMLLSEK"
     gene            complement(1877..2785)
                     /locus_tag="PKZ52_15695"
     CDS             complement(1877..2785)
                     /locus_tag="PKZ52_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61265.1"
                     /translation="MKKYICGVLLSMTFLTSLMAGAKEPVMFERVIQLPHAELRFSMA
                     EDFSRDMPAAPLVEKLTVDMLSGLKTPGDRFVIGRRWWDLKPPGWFKKPLGSMQMTIS
                     IGGGIDVGQLNFCKMTQSEFLIFYRESLVNKWKPHNEGIASADLLKFGVNVYPFFSGR
                     GQEYIPYFEFKKNNKGIIWLSSGAGQDANMYYYFSAPVGAANFVEVEFIGAPDLNGSP
                     YLFQNLVMERARLINESVLVAFGKENCLALGGGNWIEANSVELLKPDPSLLPKPIPVE
                     EFLRKNGLKELPGTRELLEGSKNNLF"
BASE COUNT          878 a          790 c          502 g          796 t
ORIGIN      
        1 ccgaagcatc tactcgggcg ctatccaata ggccgacttt ctcaccgagc aatttaatat
       61 ccccaccttt gcccgcacca tgggcgatgg ccgttacagt gccttggtct tttgtttcgt
      121 taatgttttg ggcggtaatt tcaatgtgac ccgcatggct ggtttggctg tcggcctgaa
      181 tactgccttg gttagtaatg ttggccccca gcaacaccgc ttcgcccgcc tgcgccgctg
      241 cacctacatt aatggcgccg gtatttttta tatctgcacc cgcccccagc gtaaaactgc
      301 catcggcatt taccaccacg cttttagctg cggataattc acctacatta accgcttggc
      361 taaaaatatc tttactggtg ctggccgtca ttaatacctt gctgcctgcg gctttaacct
      421 ccccagcatt aataatggca gcatccacac ccaggtcttt ttgtaaaata gcctcagcaa
      481 cccttacgcc cactaagcca ttgggctcaa aagttacaat ggcctcttta cccgctgcca
      541 cattcacgcg ccccaactgg gcgctaatta agcctttgtt ttcgacactt tggccgagca
      601 aactaatcgt gccgcccgtg gcggcattta acaggccgga attaatgacc cggccttcac
      661 tgccctctaa cgcattgagg gtaaattggc catttaaata atcctgatcg ctcatctgca
      721 aagcggaggc aactaaacca cctacattaa cgctggcgcc ttccgtaaaa attagcccat
      781 tggggttaat taacaccacc tgcccattag catcaatacg gccttgaatt aaagagcctt
      841 tattgctcag gatgcggtta aacgcgatag aggtgctatt cggttgaata aacaacactc
      901 gctcatcggc attaatatca aagcttttcc agtctatgct tagccggtca ctggcttgtt
      961 gaatgcgggt ttctttttct acctgctcta taacgccctg tccggcgaca atttgcccac
     1021 cttctgggcc agcccaccca gcagagccaa ataaatacaa cacaccgcct aaagcgcagc
     1081 caatcacacg tggcatataa aaaaaacaag gcataccttc cgctccatta cacatcaaca
     1141 attaaggggc aaccacgtgg gcgcagtcag acacagataa aatgctttga aatacagtta
     1201 gaatttttgg cgcccattag cacccaacaa tactgaaatt gatttacctt atagtgaaca
     1261 cccgaaccca ctacacaggc atcagcatga aaaaacgcag tatattttct cgtatcgcca
     1321 tcttaaacga aggaagggaa ccatttctta attacctcag aaacatcact ccacaagcaa
     1381 ttttgctaag ctttgcactg ctcgcaacat ataaaagcac aattaaacca cagcaccaca
     1441 ccgggcttat ggctttttca ttactattct tttcgatctt tgcagccgca agctatgcca
     1501 acattacgct attctataaa aacatcttca aagacagcct aaagaaaaac aacgcctcga
     1561 ttataagact atgcagaaaa cacaaattta caaaatacaa acaactaata atgattccat
     1621 ggctaacatt aaaatataaa tgggtagaat taatcgaact gctttgcaca gtcgtgtttc
     1681 tacagagcac tataatgatt gtgttttatt tagcagggct acaatattca gccatgctac
     1741 tttcagaaaa ataaccgcat aaatgatcga cagtggcttc tagccactgt cttgctcaca
     1801 caaaccaatt aaaatactat taacttagca gctccaacgt gtcaactaac ttaataccaa
     1861 atagtaatca cgcacactaa aatagattat ttttgctccc ctcaagcaac tctcgcgtac
     1921 cgggcaattc ttttaatcca tttttccgga gaaattcttc gaccggaata ggtttcggca
     1981 agagcgaagg gtcaggtttt agcaactcca ccgaattggc ttcaatccag tttcccccac
     2041 ctaaagccaa acaattttcc tttccaaacg ccaccaaaac gctttcattg atcagccgtg
     2101 ctctctccat tactaaattt tggaataaat atggggagcc attcaaatca ggggcgccga
     2161 tgaactcaac ctccacgaaa ttagccgcac cgactggagc agaaaaataa taatacatat
     2221 tcgcatcctg acctgcccct gaagaaagcc atataattcc tttattattt ttcttaaatt
     2281 caaaatatgg tatatattct tgtcctcttc cgctaaaaaa tgggtataca ttcacgccaa
     2341 actttaataa atcagcagat gcaattcctt cattatgagg cttccactta ttgactaaag
     2401 attctctata aaaaatcaaa aattcagatt gtgtcatctt gcaaaaattc agctggccaa
     2461 catcaatacc accgccaata gatatagtca tttgcatgct gcccaatggc tttttaaacc
     2521 aaccgggtgg ttttagatcc caccaccgtc gcccgataac aaaacgatct ccaggcgtct
     2581 tcaacccact caacatatca actgtcaact tttccactaa aggcgctgca ggcatatccc
     2641 tcgaaaaatc ttcagccata ctaaaacgca attcagcgtg tgggagctga attactcgct
     2701 cgaacattac aggctcttta gcgccagcca taagcgatgt aagaaacgtc atagataaca
     2761 aaactccaca aatatatttt ttcataattt ttatttcaaa tagttaggta ttgccttaac
     2821 tgctttacgc cctaaccgat caaccgcatg aaaccctttt tgaacagtgc tacgcccacg
     2881 accggcccca taagtcattt ctctattcca cgcatcataa tctttatgtt gcatcgtatg
     2941 agtactttga ttcaccgagc cactaa
//
LOCUS       DAOQGS010000526         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20181_length_2956_cov_4.341951, whole genome shotgun sequence.
ACCESSION   DAOQGS010000526 DAOQGS010000000
VERSION     DAOQGS010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20181_length_2956_cov_4.341951"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="PKZ52_15700"
     CDS             complement(<1..1661)
                     /locus_tag="PKZ52_15700"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNG61266.1"
                     /translation="MLFACGGVVEPTPSSSSSSSSSSSSSGKASSSSSSSSSSSTGRT
                     SSSSSSSSSSSSGRTSSSSSSSSSSSSTGSVLGNVATGATQFEAQCSVCHDHQGSGAF
                     TAPNKPNFNIKTVQARLGDIGLVDFIAKSMPPGNPSKCATADNCAANTAAYMVSLASQ
                     LASSCNTSTQPYGMRSLRLLTARELKNSLIDLGIAQASELTDDMFTQDATYTKSKFPI
                     HTHVTTAVDDNRMDAMLYLADKITATSATRLRNNWGCGTNATNCANSFLTLAERIFRR
                     PLTDAEKSTYNAFFTKYGAQVGSEIALAAAITSPQFLYRSELGTKVSDARNNSSNFGT
                     QINLQNLDQSAYILDNYEYATLLAYMYTGSTPDQTLMQAAKNNQLNTESAVNSQIDRL
                     LQTSRGREHITEFGANWMRTDDALKARRPNNPEFTDDIKNDMAKEVRELFGYVFFTAN
                     TSFAQFYAADYSVINSRLASYYGVSNFSGGTSDWKATTLPNRGGIIGTGAFSVGNSQP
                     DRSGPIKKAVDIRELMLCHHIGAPPTDLTTTSKREELVKAAAQRE"
     gene            2447..>2956
                     /locus_tag="PKZ52_15705"
     CDS             2447..>2956
                     /locus_tag="PKZ52_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61267.1"
                     /translation="MKKHLCEYRGAMAVLLCAAASIFVVACGASKTPPTVYAISGQLN
                     DTGSTLCASQDQVQQACPQAGLAGQDAEFGRDSNKALKKRGGGVAGFDWTKLDAKGQP
                     LAQQSVAWADNGSEAAGSHWSCVQDNVTGLIWEIKETNPEHPRYFGHTYSWWMASEQL
                     NGGFTYHLTP"
BASE COUNT          681 a          631 c          858 g          786 t
ORIGIN      
        1 ttttcacgct gggcagcggc tttaactaac tcttcacgct tagaagtagt ggtcaggtca
       61 gttgggggcg ctcctatatg atggcacagc attaattcac gaatgtctac agcctttttg
      121 atggggccgg agcggtcagg ttgtgagttg cccaccgaga aggcaccggt gccaataata
      181 ccgccgcggt taggcagggt agtggccttc caatcgctgg tgccgccgct gaaattgctc
      241 acaccgtagt aagacgccag gcggctgtta ataacactgt aatcagcggc atagaactgt
      301 gcgaaggacg tgttagccgt gaagaataca taaccaaaca gttcgcgcac ttctttggcc
      361 atgtcgtttt tgatgtcatc ggtgaactca ggattgttgg ggcgacgggc ttttaaggcg
      421 tcgtcagtac gcatccagtt ggcgccgaat tcagtgatgt gctcacggcc gcgcgaggtt
      481 tgcaacaggc ggtcgatttg cgagttaacc gcagattcgg tgttcaactg attattctta
      541 gctgcttgca ttagggtttg gtctggggta ctgccggtat acatataggc caacagagtt
      601 gcgtactcgt agttgtccaa gatataggcg ctttgatcca agttctgtag gttgatctga
      661 gtaccgaagt tggatgagtt gttgcgcgca tcgctcacct tagttcccag ctcagagcga
      721 tagaggaact gtggagaggt gatagcagcg gccagggcaa tttcggaacc aacttgtgcg
      781 ccgtacttag tgaagaaggc gttgtaggtc gacttctcag catcggtcag tgggcgacgg
      841 aagatgcgct cagccaaagt taaaaatgaa ttggcgcagt tggtggcatt ggtgccacag
      901 ccccaattgt tgcgcagacg ggtggctgag gtggcggtga tcttatccgc aagatacaac
      961 atggcatcca tgcggttatc gtctacagct gtagtcacgt gagtgtgaat ggggaactta
     1021 ctcttagtgt aggtcgcatc ctgagtaaac atgtcatcgg tcaattcact ggcttgggcg
     1081 atgcccaaat caatcaatga gtttttcaac tccctagcgg tgagtaggcg caaagagcgc
     1141 atgccgtatg gctgagtgct cgtgttgcac gaacttgcca actgagaagc taaagacacc
     1201 atgtaggcgg cggtgttggc tgcgcagttg tcggcagtgg cacatttgct ggggttgccc
     1261 gggggcattg acttcgcaat gaagtcaacc aagcctatat cgccgagccg tgcttgtact
     1321 gtcttaatat tgaagtttgg tttgtttggg gcagtaaatg ccccgctgcc ttggtgatcg
     1381 tggcaaacgc tgcactgagc ttcaaattga gtagcacccg tagcgacatt gcccaacacc
     1441 gacccagttg aagagcttga ggagctggaa gacgatgagc ttgatgttct accactggag
     1501 gatgatgaag acgacgaaga agagctagat gttctgccag tcgaggagga tgaagacgac
     1561 gaggaagagc tagaggcttt acccgaggaa gacgagctgg aagatgagct gctagagctt
     1621 gagggggtcg gctcaacgac tcctccacag gcgaatagca aggcggtagt cgccagtact
     1681 cctgtaaatt tgatagctcg gctagctttc gatagcgacc ttatactgag catagttatt
     1741 accctattag ttttagtatt gacccaccgc cttaagcggt gggctggtga agatgcggtg
     1801 cgcaattttg ttatgttccg aacccaggtt acctcaggcc agggtaaatt tctgtaatgg
     1861 cattcatgat ttgggttgat aaatatctca aaatgaaaat tatctggatt gatatagcta
     1921 aaaacggcgt taaagggtct gctcgtagct tgagaactgt tgttttatag gataaaagcg
     1981 cttgaggagt ctgagttggg tttgaatttc tctgctgttt tgctaatgag taattaccat
     2041 taattcggag gtagaaatta cctttggtat tagattgctg gtgagcttaa gcagctgcag
     2101 ctggttgcag ggttaaaatg ctcaccgttg tttttagggg ggcgaggcgg ccattttttg
     2161 ccgccttggc ttgtgctgct gggcagtgct taagtggagg ttcgctgagt aaaaaactgg
     2221 gtgtccctca caagtcggtc acgtttgtgt cactctgcag cagcagttga ttaaattcgc
     2281 tttgcttcct gcgtttctgc aattaaagtc aactgcgcgc ttacgtttga tggctgtggc
     2341 gggattaatt ttcggcattt atttttgtgt gttgggtgca atctgcgtgg gctgtgcgca
     2401 attaaattag cgcagttctt tttatcggtt ttattgtcgg tgcgtgatga aaaaacattt
     2461 atgtgaatat cgcggtgcaa tggcagtgtt attgtgcgcc gcggcctcta ttttcgtggt
     2521 ggcttgtggc gccagcaaaa caccccccac tgtttatgct attagcggtc agctcaacga
     2581 taccgggtca accctgtgcg ccagccaaga tcaagtacaa caggcgtgtc cgcaggcggg
     2641 attggctggg caagatgctg agtttggccg agattctaat aaagccttga aaaaacgcgg
     2701 cggcggggtg gcaggctttg attggactaa attagatgcc aaaggccaac cgctagcgca
     2761 acaatcagtt gcctgggctg ataacggctc ggaagctgcg ggcagccatt ggtcctgtgt
     2821 gcaggacaat gtgaccgggc tgatttggga gataaaagaa accaatcccg aacacccgcg
     2881 ttattttggt cacacctaca gttggtggat ggcctccgag caattgaacg gtggttttac
     2941 ctatcatttg acacct
//
LOCUS       DAOQGS010000527         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20289_length_2947_cov_4.013140, whole genome shotgun sequence.
ACCESSION   DAOQGS010000527 DAOQGS010000000
VERSION     DAOQGS010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20289_length_2947_cov_4.013140"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKZ52_15710"
     CDS             complement(<1..388)
                     /locus_tag="PKZ52_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNG61268.1"
                     /translation="MGQAHAHNHQHCIDTAIARAKAICAEAGAKLTAQRQLVLQLVWQ
                     SHKPLGAYPLMSQLQQLTGKIVAPPTVYRALEFLLEHGLIHRVHSLNAFVGCDHPGQP
                     HSSQLLICRNCGDAQELDSATIDASIA"
     gene            380..1318
                     /locus_tag="PKZ52_15715"
     CDS             380..1318
                     /locus_tag="PKZ52_15715"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNG61269.1"
                     /translation="MSHGKVSVKEKTQWYNIGDFSAMWVCMGRRIIGMWLTAAAVLCG
                     LQAAHLQAGEPLRVVSSIAPVNLLVQGLAGPEVSAQSLLNAKADPHEFALRVSERSLL
                     SEADLVIWIGPNLERFLVKALPDTRAQLELSQLPGLTWPQEQPRVDLHLWLNPANAKL
                     MASAIAERLCALLPASAPAIQQRLSLLHARIDSVSAQIKTRLAPVQAVPFGVAHDGYG
                     HWVSYFGLNQVAALSVLPEQSLGAKHLSAVQQKLQGARCVISDFSEGNQQKLQALLSL
                     PIQVADPLAQARPYADYGEFLLALADVFSACLSPAG"
     gene            1385..2002
                     /locus_tag="PKZ52_15720"
     CDS             1385..2002
                     /locus_tag="PKZ52_15720"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNG61270.1"
                     /translation="MLSQIGRGFWVMIIAWVLSSPSFAATTGPHQVVEKVTQEMMAVV
                     KGGDQALKANPEQYYEKVGKVLDSVVSFEFIAKNVMGPTWEQASPAQRSQFTTAFRSG
                     MVKTLAKGMGNYQDLKIQVLPPKGDISGQKRVDVQQEVAGTDKTHQISYSMALDKNNE
                     WKLVNVVLNGVNLGKSFRDQFTQALKQNNNNIDAVTQGWNSEPAK"
     gene            2208..2450
                     /locus_tag="PKZ52_15725"
     CDS             2208..2450
                     /locus_tag="PKZ52_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNG61271.1"
                     /translation="MQEHEVITAVSAAIADAQVQVRFEGNHVHLRVISPAFTGLSPVK
                     KQQLVYGALKDAIASGAIHAVHMQTLTPEEWAAQAQ"
     gene            2484..>2947
                     /locus_tag="PKZ52_15730"
     CDS             2484..>2947
                     /locus_tag="PKZ52_15730"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600848.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNG61272.1"
                     /translation="MDKLLIKGPCQISGEVRISGSKNSGLPILAATLLAQTPVLLQNL
                     PHLNDITTMLALLRCMGVGITIDERMGVEVDASNITDFSAPYDLVRTMRASILVLGPL
                     LARFGQADVSFPGGCAIGSRPVDIHLKGLEAMGAQIEIDGGYIRARAPEGLKG"
BASE COUNT          617 a          857 c          859 g          614 t
ORIGIN      
        1 gggcgatgga ggcgtcgatg gtggcgctgt ccagctcctg ggcatcgccg cagttgcggc
       61 aaattaacaa ttggcttgaa tggggctggc ctgggtgatc gcagcccacg aaagcgttca
      121 ggctatgtac ccggtgaatc agaccgtgct ccaagagaaa ttccagcgcc cggtacaccg
      181 tgggtggggc gactatcttg ccggtcagct gttgcaattg gctcatcagc ggataggcgc
      241 ccaggggttt gtggctctgc cacaccagct gcaacaccag ctgccgctga gccgtcagct
      301 tcgccccggc ctcggcacag atggctttag cgcgcgctat agcggtgtcg atgcagtgtt
      361 ggtgattgtg ggcgtgggct tgtcccatgg caaagtctcg gtgaaggaaa aaacgcaatg
      421 gtataacata ggcgattttt cagccatgtg ggtgtgtatg ggcaggcgaa taatcggtat
      481 gtggctcacg gcggcggctg ttctgtgcgg gctgcaggcg gcgcatttac aggcaggaga
      541 acccctgcgg gtggtgagca gcattgcgcc ggtcaacctc ttggtacagg gtttggcagg
      601 ccccgaggtg agcgcccaga gcctgttaaa tgccaaggcc gatccccacg aattcgccct
      661 gcgggtatcc gagcgcagcc tgctcagtga agccgattta gtcatatgga tcgggccgaa
      721 cctggagcgg tttttggtta aggcgctacc agatacccgc gctcagctgg agcttagcca
      781 gctgccgggc ctcacctggc cccaggagca accgagagtg gatctgcatc tgtggctcaa
      841 tcccgctaac gccaaactca tggccagcgc cattgccgag cgcttgtgtg cgctgttgcc
      901 cgcctcggcc ccggccattc aacagcgatt gagcctatta cacgcgcgca tcgacagcgt
      961 cagcgcgcag atcaaaaccc gccttgcccc tgtgcaggcg gtgccctttg gggtcgccca
     1021 cgacggctac ggccactggg tgagttactt cgggctgaac caggtggcgg ccctgtcggt
     1081 gctgccggag cagagcctag gtgccaaaca cttatccgcc gtgcagcaaa aattacaggg
     1141 tgcgcgctgt gtaatcagcg attttagcga gggcaatcag cagaaattac aggcgctgtt
     1201 gtctctgccc atacaggtgg cggaccctct agcccaggcc cgcccctacg ccgattacgg
     1261 tgagtttctg ctggcgctgg cggatgtgtt cagcgcctgt ttaagccccg cagggtaagg
     1321 tgcctcgccc tgtagtctgc ccgtgcgcgg gtgttttctt ttaattttca acggagacta
     1381 agttatgttg agccaaatag gccgtggatt ttgggtgatg attattgctt gggtgctcag
     1441 tagcccgagt tttgcggcaa caaccggtcc ccaccaagtg gtggagaagg tcacccaaga
     1501 aatgatggcg gtggtgaaag gtggcgacca agccctcaag gccaacccag agcagtacta
     1561 tgaaaaagtc ggcaaggtgc tcgacagtgt ggtgagcttc gaattcatcg ccaaaaacgt
     1621 catgggcccc acttgggaac aggccagccc cgcccagcgc agccagttca ccaccgcgtt
     1681 tcgctccggc atggtgaaaa ccctggccaa gggcatgggc aattatcaag accttaaaat
     1741 ccaagtgctg ccccctaagg gcgatatcag cgggcaaaag cgcgtggatg tgcagcagga
     1801 agtcgccggc accgacaaaa cccaccaaat ctcctacagc atggcgctgg ataaaaacaa
     1861 cgagtggaag ctggtcaacg tggtgctcaa tggcgtcaac ctgggtaaaa gcttccgcga
     1921 tcaatttacc caggcgttaa agcagaataa caacaatatc gatgcggtaa cccagggctg
     1981 gaactctgag cccgctaagt agctgcttgt gcaagtagct gtatttttat taaagaaagg
     2041 ttaagtaaac gggcggacac aaccgcccgt ttgtttttct cctcccgcct cttcgccgcg
     2101 ctcaaaccca tgtcgcgccg cctgcactat gtttttgatg tcccagcccg gcaattgcat
     2161 tacactagcg cccttttttt ggccgccgag ccccacaggc tttagttatg caagaacatg
     2221 aagtaattac cgcggtgagc gccgcaatag ccgacgccca ggtacaggtt cgttttgaag
     2281 gcaaccacgt ccacctgcgg gtgatcagcc ccgcgtttac cggcctcagc cccgtcaaaa
     2341 agcaacaatt ggtgtatggc gcccttaaag acgccatcgc ctccggcgct attcacgccg
     2401 tgcacatgca aaccctgacg cccgaagaat gggccgccca agcgcaatag cttttaccct
     2461 ccaacgtgca actacaaccg ctcatggata aattgttaat taaaggccct tgccagatca
     2521 gtggcgaggt gcgaatttcc ggctccaaaa actccggcct gccgatactc gccgctactt
     2581 tgctcgccca aaccccggtt ttgttgcaaa acctgccgca tttgaacgac atcaccacca
     2641 tgctggccct gctgcgctgc atgggggtgg gcatcaccat cgacgagcgc atgggcgtgg
     2701 aagtggatgc cagcaatatc accgattttt ccgcacccta tgacctggtg cgcactatgc
     2761 gggcgtctat tctcgtgctg gggccgctgc tggcgcgctt tggccaggcc gatgtgtcct
     2821 ttcccggcgg ctgcgccata ggctcccgcc cggtggatat tcacctcaaa ggcttggaag
     2881 ccatgggcgc ccagattgaa atcgacggcg gctatattcg cgctcgcgcc cctgagggcc
     2941 ttaaggg
//
LOCUS       DAOQGS010000528         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20310_length_2945_cov_4.226990, whole genome shotgun sequence.
ACCESSION   DAOQGS010000528 DAOQGS010000000
VERSION     DAOQGS010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20310_length_2945_cov_4.226990"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKZ52_15735"
     CDS             <1..900
                     /locus_tag="PKZ52_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNG61273.1"
                     /translation="TRGNQVLFGGQPGSIAGMSLFWSSFPEGSKFYTSGVVNTLKTTW
                     NAKLVRAAMGVDATGGYLTDPTNNLNRVKTVVDAAIANDMYVIIDWHSHNAEDYKAQA
                     IAFFQNMARTYGTRNNVIYEIYNEPDCPTSAATCGWDNKTSWATIKAYAVDVIAAIRA
                     IDPDNLIIVGTPFYSQFVDEASENPISGVNIAYTLHFYAASHKEQERGRARTALKNGI
                     PLFVTEWGTVGASGDGAVDSAETDTWMQFLYDNNISHANWSVSDKAEGASILKPGSNK
                     VNGWTDTDLTASGNLVKSHILGW"
     gene            complement(1079..2116)
                     /locus_tag="PKZ52_15740"
     CDS             complement(1079..2116)
                     /locus_tag="PKZ52_15740"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNG61274.1"
                     /translation="MASAEKNSLNTAPTEFDNPIIKYDTPDPTRQVGAIIYTADPVAL
                     VVDDWVYLYTGHDEQTQSGVDYRMYDYRLWASQDLMHWQNKGAVLRYSDFAWARGSEK
                     TGNAYAGHVTHRKGADGKANYYFYTTLEGGQKDFGFAIGVAVSDKPEGPFVDPRGMPM
                     ILLSDTAQYQKHSWRNIDPAVFIDDDGRAYLYWGNKQLWWVELEVDLVHLKGEAYSRD
                     ASGKMLNRSTQNVNINTVDTLPNFEEAPFISKHYGRYYLTYAAGFPESLAYAISDSPR
                     GPWHYKGILMDPLPNTSTIHPALINFKGDAYLFYHDAALPGGGSYRRSTCLKKLHFNA
                     DGTIQKITFDK"
     gene            complement(2708..>2945)
                     /locus_tag="PKZ52_15745"
     CDS             complement(2708..>2945)
                     /locus_tag="PKZ52_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61275.1"
                     /translation="RQIKHCIQEHKKADTRTSDGKERARRLQQKAETLNKAADRIDEF
                     LANEQPKIGQGKTKKEIKRVTRIDFFIHVTRNPD"
BASE COUNT          725 a          767 c          695 g          758 t
ORIGIN      
        1 acccgtggca accaggtgtt atttggcggg cagcccggca gtatcgctgg tatgagcctg
       61 ttctggtcga gctttcctga aggcagcaag ttttacacaa gcggtgtggt gaataccctt
      121 aaaactactt ggaacgcgaa actagtgcgc gccgccatgg gtgtggatgc cacaggtggt
      181 tacctaaccg atccgaccaa taacctcaac cgtgtgaaaa ccgtagtgga cgccgcgatc
      241 gccaacgaca tgtacgtgat catcgactgg cacagccaca atgcggaaga ctacaaagct
      301 caggccatcg ccttcttcca aaatatggcg cgcacctacg gcacgcgcaa caacgtcatc
      361 tatgagatct acaacgagcc ggattgcccc accagcgccg caacctgtgg ctgggataac
      421 aaaaccagct gggccaccat caaggcctat gctgtagacg tgatcgccgc cattcgcgcc
      481 atagacccag acaacctgat tattgtcggc acgccgtttt attcgcagtt tgtggatgag
      541 gcttccgaga accccatcag cggcgtgaac atcgcctaca ccctgcactt ctacgccgcc
      601 tcccacaaag agcaagagcg cggccgcgcc cgcaccgcgc tgaagaacgg cataccgctg
      661 tttgtaaccg agtggggaac tgtaggcgcc agtggtgatg gcgctgtgga tagcgccgag
      721 accgacacct ggatgcaatt cctctacgac aacaacatca gccacgccaa ctggtccgta
      781 agcgacaagg ccgagggcgc atccatcctc aagcctggct cgaataaagt gaacggctgg
      841 acggatacag acctgactgc ctccggcaac ctggtgaaga gccacatttt gggctggtaa
      901 ttctagggat gataaaacgg ctggttgctt aaggcttcca gccgtttttt tatgcgcgca
      961 tttttgtatt tggataatgt gtgctgtctt tcgctcaagc ttatgcagcg caaaagccat
     1021 ccattagtta cacctaaaaa ctgcagttgc accctactgc ggtttttaga ttttgccatc
     1081 atttatcaaa cgtaattttt tgaattgtgc catctgcatt aaagtgcagt tttttaaggc
     1141 aggtggacct gcgataactg ccgcctcctg gcaaagcggc atcgtgataa aacagatagg
     1201 catcgccctt aaagttaatt aatgccgggt ggatggtgga ggtgttcggc agtgggtcca
     1261 ttaaaatgcc tttatagtgc caaggccccc tgggtgaatc gctgatggca taggcaaggc
     1321 tttcagggaa gcctgccgcg taagtcaaat aatagcggcc atagtgtttt gaaataaaag
     1381 gcgcctcctc aaaattaggc aaagtatcca ccgtattgat gttcacattt tgggtcgatc
     1441 tatttagcat tttaccgctg gcatcccggc tgtaagcctc gccctttaaa tgcaccaagt
     1501 caacctctaa ctccacccac cacaattgtt tattgcccca ataaagatag gcgcggccgt
     1561 catcatcaat aaataccgct gggtctatat tgcgccagga gtgtttttga tattgcgcgg
     1621 tatcgctaag caaaatcatc ggcataccgc gcgggtccac aaacggcccc tcgggtttat
     1681 ccgagacagc aacaccaatt gcaaaaccaa aatctttttg gccgccctct agggtggtat
     1741 aaaaataata gttggctttg ccatccgcgc ctttgcgatg ggtaacgtgc ccggcgtagg
     1801 cattacccgt tttttcactg ccccgcgccc aggcaaaatc cgaataacgc agcaccgcac
     1861 ctttattctg ccaatgcatt aaatcctggc tggcccataa acgataatcg tacatacgat
     1921 agtccacccc gctctgggtt tgctcatcgt ggccggtgta caaataaacc caatcatcaa
     1981 ctaccagcgc aaccgggtcc gccgtataaa taatcgcgcc cacctgccgg gtggggtccg
     2041 gggtatcgta tttaatgatg ggattatcaa attccgttgg cgcggtatta agggaatttt
     2101 tttccgcact tgccattggt ttaatcggtt gcgtacagct taagcttgtc aacagcgctg
     2161 gcagtgccaa taacgcaaca ctatttttta aatacacctt catggcttac tcactgcttc
     2221 ttggttatca aagtgattta ggaaatttag aataatgctg gattacttcg cgaacccact
     2281 actgcggctt acccaccttg agggttcacc gatgtgtcac cacagcagtt ttaacggtaa
     2341 attgcgcgct caacataaag acaaaatgtt gaataaggcc agtcctttac ctgggttgcc
     2401 aagccatatt taagcggatt gcggtgaagg taatgaatgt ggcattccag atcccactta
     2461 tcccgcagca aatgctccca gtaccgccgc tgccaaatac ctatttcatc atttttattt
     2521 cgtgaagtag agcgacattc tgttttgggc aggcttttgc taaataattt tatcagcccg
     2581 taggtcggaa aagcgcagcg ccttccgacg tgaaattttc tcgatagcac attgttaaac
     2641 catgtattgt gctaccggct tgatgtcgga aggcgctacg cttttccgac ctacatgaat
     2701 caaaaaatca atccgggtta cgcgttacat gaataaaaaa atcaatccgg gttacgcgtt
     2761 taatttcctt tttggttttg ccttggccga ttttgggttg ctcgtttgcc aaaaattcat
     2821 caatgcgatc cgcagcttta ttcaacgttt ccgctttttg ctgaagcctg cgtgcacgtt
     2881 ctttaccatc gctagtgcgg gtgtccgcct ttttgtgttc ttgaatgcag tgtttaattt
     2941 gcctt
//
LOCUS       DAOQGS010000529         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20312_length_2945_cov_3.368858, whole genome shotgun sequence.
ACCESSION   DAOQGS010000529 DAOQGS010000000
VERSION     DAOQGS010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20312_length_2945_cov_3.368858"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2945
                     /locus_tag="PKZ52_15750"
     CDS             <1..>2945
                     /locus_tag="PKZ52_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNG61276.1"
                     /translation="DGNANDIYVYGRDTFILLYGDVSVPIILQGPPSYVFYEKSDHSH
                     DDAEILEFADYSWQSKAQKASPQDFTQGDLTGDGTADLLIRGRGQYDTALLVNGKLVQ
                     DGADLVPAYLGDIDSDGRIYTRGKGWSASPVVNGNLSDRNQAISLAGGKLSVAGKFYV
                     YNASSTLVPDAGTAAATSNVPAPPAPIYAQPTLNAQDQQTMDSLGILSGQFKVNESGA
                     ATYNLPLKLPQGTAGVAPDVSIGYSSQSGDGLLGLGWSLNAGGAISRCRQTLQVDKAA
                     LPISWSSVDRYCLNGQRLLLVSGNYGMADSQYKTEIDTQITVTQKGGSGSADYFEVKA
                     KDGSTSIYGVSGTDSSEEKGYPTSTASCTTVASNGSCADKVMSWNLTQFSDRVGNKIQ
                     YVYENGTNTDQKSYRLNSIKYAFANGQSNARIQFNYEEGKKETSLAAGYQFYRDSRLA
                     SVETYQTIGGENLLRKYSFNYNGDLTKTFTDERDRLTSIVECSAESCLPPLRLTWGDN
                     LMLYPVTRSVDLKAGFYIDSSFGPNYLDVNGDGRMDFVTLDSSTGDKKYRMRYAVQGA
                     DGNFNWGTLTNGATSTSMVSFDISTTGQENFQPHQLRMRTHPVDVNNDGRSDVIVYRK
                     ESWGGVTWDLYLATPTPNAFPNDWSLVYKTSAEFPLPFGANGLSNNGNGVQFGDVDSD
                     GLIDAFFTENDRIYVSLLKRDTSSPNVNLPYKYQMPVDRGSAAGVDSFKLIPGGDFNG
                     DGRLDFIGTTRTISSNGSSCSATGIVKVFYQDTQSSFSRVETISSQQSFVNSNSWVPC
                     TAFLAGKETYPENISFADVNADGAVDVVWTLTNADNTFSKFYVNFGDGKGGFAALQTY
                     TLSRGGARNIQFSDRNSNGKIEAIVTYKNERRMYIYEWNNGAFPSSPAEVDPTSTDSG
                     TSTQFFDVNADATLDRVESTVDKVTFYHGEKALTNGLVTSFTTGRGIKTAVQYEPLGT
                     TPNYTKISN"
BASE COUNT          789 a          682 c          703 g          771 t
ORIGIN      
        1 cgacggtaat gccaacgata tttatgtcta cggtcgagac acctttatat tgctgtatgg
       61 tgatgtatcc gtgcccatta ttctacaagg gccgccaagt tatgtgtttt acgaaaagtc
      121 cgatcatagt cacgatgatg cggaaatatt ggaatttgcc gattacagct ggcaatccaa
      181 agcgcaaaag gcctccccac aggattttac tcagggtgat ttgacgggtg acggcaccgc
      241 cgatttatta attcgtggcc gaggccagta cgacaccgcg ctgctggtca atggcaagct
      301 ggtgcaagac ggtgcggatt tggtgccagc ttatttgggg gatattgaca gcgacggtcg
      361 catttatacg cgtggcaagg gctggtcggc gagcccggtg gttaacggta atctttccga
      421 ccgaaatcag gccatcagct tggctggcgg taaactttct gtagcaggta agttttatgt
      481 ttacaacgcc agctcaaccc tcgttcccga tgctggaact gccgcagcca catctaacgt
      541 gcctgcgccc cctgcgccaa tttacgccca gcccacgctc aatgcgcaag atcaacaaac
      601 aatggattct ttgggaattc tgagcgggca gttcaaagtc aatgaatccg gtgctgctac
      661 ttataacctt ccgcttaaat taccgcaggg aacagccggt gttgcgcctg acgtatccat
      721 cggttacagc agccaatcgg gcgatggttt gttgggcctc ggttggtcgt taaatgcagg
      781 cggggctatt agtaggtgcc gtcaaacgct gcaggttgat aaagcggctc tgccaattag
      841 ttggtcttcc gttgatcgct attgtttaaa tggtcagcga ttattattgg tttccggcaa
      901 ctatggtatg gctgattccc aatacaaaac cgaaatagat acgcaaatta ctgtaactca
      961 aaagggtggc agcggcagtg ctgattattt tgaagtaaaa gcgaaagatg gctccacttc
     1021 aatttacggt gtgtccggta cggatagtag tgaagaaaaa ggttacccaa ccagcacagc
     1081 gtcatgtaca actgttgcga gtaatggtag ctgcgcagat aaggttatga gctggaatct
     1141 tacgcaattt agcgaccgcg tcggcaataa aatccagtat gtttatgaaa atggcactaa
     1201 tacagaccaa aaatcatatc gtctcaattc gattaaatat gcctttgcca atggccagtc
     1261 caatgcgcgc attcaattta attatgaaga gggtaagaaa gaaacatcac tagcggcggg
     1321 ttatcaattt tatcgtgaca gtcgtttagc tagtgtagaa acctatcaaa ccattggtgg
     1381 tgaaaattta ttacgcaaat attcatttaa ttacaatggg gatttaacta aaacctttac
     1441 cgacgaacgc gatcgcttaa ctagcatcgt cgaatgctct gcagaatcct gcttaccgcc
     1501 tttgcgtctt acatggggcg acaatctgat gttgtatcca gtgactcgct cggtggacct
     1561 caaagctggc ttttacattg attcttcctt tggccctaat tatctggacg tgaatggcga
     1621 tggccgcatg gattttgtaa ctctggatag cagtactggg gacaaaaagt accgcatgcg
     1681 ttacgccgtg cagggggctg acggcaattt taattggggt acattaacca acggggcgac
     1741 cagcacctcg atggtttcct ttgatatatc caccacaggg caagagaatt tccaacctca
     1801 tcaattgcgc atgcgcaccc atccagtcga cgtcaataat gacgggcgct ctgatgttat
     1861 tgtttatcga aaagaatcat gggggggggt cacatgggat ttatacttag ccacgccgac
     1921 acctaatgcg tttcccaatg actggtcctt ggtctacaaa accagcgcgg agtttcccct
     1981 tccctttggt gcgaatggat tatccaataa cggtaatggc gtgcaatttg gcgatgtcga
     2041 ttcagacggt ttgattgatg ctttttttac cgaaaacgac agaatttatg tttcactact
     2101 caagcgcgac acaagttctc ccaatgtaaa tttgccctac aagtatcaaa tgcccgttga
     2161 tcgtggctcg gccgcaggcg tagattcttt caaattaatt cccggtggag attttaatgg
     2221 ggacggtcgg ctggatttta ttggcaccac aagaactatt tcttcaaacg gttctagctg
     2281 tagtgctacc ggcatagtca aagtgttcta ccaggacacc caatcttctt tctcaagagt
     2341 tgaaaccata agctctcaac aatctttcgt taattcaaac agctgggtac cctgtacagc
     2401 atttttagct ggtaaagaaa cctatcctga aaatataagt tttgctgatg tcaatgcgga
     2461 tggtgcggta gatgtggtgt ggacactaac caacgcagat aatacttttt caaagtttta
     2521 cgtaaacttt ggtgacggca aaggcggctt tgctgcactc cagacttaca cactttcacg
     2581 gggcggtgcg cgcaatattc aattttccga ccgcaattca aatggaaaaa ttgaagccat
     2641 cgtgacctac aaaaacgaac gacggatgta tatatatgaa tggaacaatg gggctttccc
     2701 tagctcgcca gcagaggtgg atccaaccag cacagattct ggaaccagca cacaattttt
     2761 tgacgtgaat gccgatgcca ccttagatcg ggttgaatca acggttgaca aagtcacctt
     2821 ctaccatggc gaaaaagcac tcaccaacgg cctggtcacc agcttcacca ccggccgagg
     2881 cattaaaacc gccgtccagt acgagccatt aggcacaacg ccaaactaca ccaagattag
     2941 caata
//
LOCUS       DAOQGS010000530         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20384_length_2938_cov_4.828304, whole genome shotgun sequence.
ACCESSION   DAOQGS010000530 DAOQGS010000000
VERSION     DAOQGS010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20384_length_2938_cov_4.828304"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKZ52_15755"
     CDS             complement(<1..274)
                     /locus_tag="PKZ52_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61277.1"
                     /translation="MNQWNCVGAAKVKLFGVAGVVALLTACGGGGGGKNTATNTVTEP
                     TTPVVSGSNNSKNTLSSSSSSSSSSSSNSSSNSSSSSSSSSSGAVAQ"
     gene            complement(1219..1704)
                     /locus_tag="PKZ52_15760"
     CDS             complement(1219..1704)
                     /locus_tag="PKZ52_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNG61278.1"
                     /translation="MRQAGFTLIELMVVLTIVGILASIAIPEYAPYVRRAEIAEAFSM
                     ASGAQRAVEEYYKDKQGFPRDNLQAGAAPAEKLIGNYIQRTELEDGAVHVTFGFKAGN
                     GLKDKILTLRPAFVEDSPSTPIAWLCGFDEPVPGMTATAKNRTNIDSTILPVRCRKSR
                     E"
     gene            complement(1701..2549)
                     /locus_tag="PKZ52_15765"
     CDS             complement(1701..2549)
                     /locus_tag="PKZ52_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61279.1"
                     /translation="MLTRLPIIDYLMSLYLLQFLCRQCIHINTQGGPQRDLLSVDEQM
                     PEFIKSQFGAPIGSEELDGLIYTRIAKLPSVGEQQAAINFYGCLNLPTFHTEQRRLRM
                     GFGYLLALSLALFCMNSIFTLKVVPSLRSLFPEVDAQHLIAPFTYFSFAPALSLGLLI
                     VVGVLSLRVLEYASLGRQVHKSWLDHCLPSRLFELHNFVNQLLLSAVLPEAERHEVSN
                     WLWRNTRDPILEHQLLISHQFNKLIDEILRWQKCAKVMISCVVIAFIVFIYMNCYLSI
                     FTKVAQ"
BASE COUNT          856 a          691 c          636 g          755 t
ORIGIN      
        1 cttgtgccac agcaccagaa gaactcgacg atgaactgga ggaggaattt gatgaagagt
       61 ttgaactgct agaagagcta gacgaacttg aactgctcag ggtgttttta ctgttattgc
      121 tgccactgac cacaggcgtt gttggctctg taaccgtatt agtcgccgta tttttaccgc
      181 cgccgccacc gccacaggcg gttaataagg ctacaacacc agctacacca aacagcttaa
      241 ccttagccgc accaacgcaa ttccactgat tcatctttaa tccccgggac gaatacccaa
      301 tgtccggcgg gctgtgcgct tgcgcaccgt accgcatgtg agtcaaatat cagcgcagcc
      361 ttaactacgc cctgagggtt tttgccaccc tttgtttgag cctagttgag ttggttataa
      421 gttgtataaa cggcttttgt atggaatggg cattctttag atcaaaatgc agcaagccgt
      481 aaagcgtgcc aaaaaatggc agaaaacccg gtaaaaacag gcaaaaaatt aatccgcaga
      541 atttcgcttg cacttaaaca ggcagtgatt aaaaccgcca ctggtgattt attgatcgct
      601 tatagattta aaatctaact aaggaagggg tgtggttatg gcatttagga tgatttgaga
      661 ggggcgttta aaaaaattga ttttttagtt ataagaattg gtggcgctga aaattggttc
      721 tggcacaggg ccaactcctg tgagaaagga ataaatgccg ataaaagaag aaaatacagc
      781 agacctatgt caataactca tcaaaaatta gcaaaataat cccggcctta tcttaaacgc
      841 caaaacaacg ttattatttg gcttacaagg tcgatatagc caaagccctt gacgcgacaa
      901 gctcgctttc gtgccgggcc aacccggagc tgtttattat ttagagacat tggtcttggt
      961 ctagggatga atgcagatta ggtatctatc ggctaaccag tagcttccta ctttgcgatc
     1021 ttaagcacca cattgcatta cgtgcctcca atttacactg agagcttccc gttttcgccg
     1081 ctttctcgca attaaaatag cagctttgcg cttttcaatt cggacattca gttcgcgata
     1141 gaaatctctg attggtaatg cgacgtgcaa tactaaggca tttacagcac atcaactaat
     1201 ggttacacaa tattaatttt actcgcgaga tttgcggcac cttacaggca aaatcgtcga
     1261 gtcgatattg gtccgattct tcgctgtggc ggtcatacca ggcacaggct catcaaaccc
     1321 acaaagccaa gcgattggcg tgcttgggct atcctcaaca aatgcaggcc gcaacgtcaa
     1381 aattttatct ttgagcccat tccctgcttt aaacccaaag gttacatgaa cggcgccatc
     1441 ctcaagttct gtcctttgaa tgtaattgcc tattaatttt tcagcagggg ctgcgcctgc
     1501 ctgtaggtta tccctcggaa agccctgctt atccttataa tattcctcaa ctgctcgttg
     1561 cgcgccggag gccatactaa atgcctctgc tatttctgcc cgccttacgt aaggcgcata
     1621 ttcagggata gcaatcgacg ccaagatgcc gacaatggtt aaaaccacca tcaattctat
     1681 caatgtaaaa cctgcctgcc tcattgcgcc accttggtaa atattgacaa ataacaattc
     1741 atataaataa agacaataaa cgcgattaca acacaactaa tcattacctt tgcacatttt
     1801 tgccagcgta ggatctcatc gattaattta ttaaactgat gggatattag taattgatgc
     1861 tccaaaattg ggtcacgcgt attacgccaa agccaattag atacctcatg acgctcagct
     1921 tccggtaaga ctgccgataa taataactga ttgacaaagt tgtgcagctc aaacagcctg
     1981 gaaggcaaac aatgatccag ccaggatttg tgcacctgcc tcccgagtga agcatattcc
     2041 aatactcgca gactcaagac acctaccaca ataagcaagc ccaagcttaa tgctggcgcg
     2101 aagctgaaat aagtaaacgg ggcgattaaa tgctgggcat caacttccgg aaataaactg
     2161 cgcagggatg gtaccacctt gagtgtaaat atcgagttca tgcaaaaaag ggctaggctt
     2221 agagccaata agtaaccgaa gcccattctg agccttctct gctcagtgtg aaatgtaggc
     2281 aaattaaggc acccataaaa attgattgcc gcctgttgtt caccaaccga aggtaacttc
     2341 gcgattcgcg tataaatcaa cccatccagt tcttcagacc ctatgggagc tccgaactgg
     2401 gactttataa attcaggcat ttgctcatca acagataata aatccctctg cgggccaccc
     2461 tgtgtattaa tatgaatgca ctggcgacat aaaaattgaa gtagataaag gctcatgagg
     2521 taatcaatta ttgggagacg cgtaagcata ccccagccgc ctttgcatga ccagttgagg
     2581 cgatagaggt gtaactattc aggatcttat gtagcttgag gtaatagctg cactgacggg
     2641 taccaattac gtgcctgttt tagcaccgtg gctcggcaat actgcccgca gcggcaacta
     2701 catataagtg cagacacgcc gtgaataatc catgtaggct cgtcaaaaag gtccctcttt
     2761 ttgacggtct gcactaatct atagccacca ctgctacacc gcgaatcaat tatgccgcat
     2821 ggctaagtcc tacagaattt gtcgccagcg cggatagggt aataaggagg actgaatact
     2881 tacctaggga acctctgaaa aatgtaggcg gcgaagctct ggctaggcga atttggca
//
LOCUS       DAOQGS010000531         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20398_length_2937_cov_4.282096, whole genome shotgun sequence.
ACCESSION   DAOQGS010000531 DAOQGS010000000
VERSION     DAOQGS010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20398_length_2937_cov_4.282096"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="PKZ52_15770"
     CDS             <1..102
                     /locus_tag="PKZ52_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HNG61280.1"
                     /translation="IKGFNSMPPKGTCADCSDDEIKGAVKYMVDKAK"
     gene            318..764
                     /locus_tag="PKZ52_15775"
     CDS             318..764
                     /locus_tag="PKZ52_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61281.1"
                     /translation="MTVYCYSKTKQAVLYLLLACLPVVGLMCFYLALNMWEAAQWFWV
                     GLWGMISAVFLGFFFVGLRMRSFIHTGIALTEEGLEFHQGAVNKLHPWSEVGAVKSWP
                     WIQLLVVFDAKGRVLLPVDHMLTNFPAFKQALEARLTQPAVASEIV"
     gene            complement(780..2663)
                     /locus_tag="PKZ52_15780"
     CDS             complement(780..2663)
                     /locus_tag="PKZ52_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNG61282.1"
                     /translation="MTDITQFSDFALKPTLLSALKEIGYEQPSPIQAQAIPSLLNGHD
                     VLGMAQTGTGKTAAFALPVLNAMDLKKRHTQALILTPTRELAIQVAEAFQKYAHNLKD
                     FHVLPIYGGQSMLSQLRALERGVHIVVGTPGRVMDHLRRKSLDLDGITHVVLDEADEM
                     LRMGFIDDVEWILEQVPTDRQTALFSATMPKQIQKVAQTYLNNPVTIQIESKTTTVMN
                     IDQQGWIVKGVNKLDALTRILEIEEYDGVIIFVRTKTATVELAEKLEARGHATSALNG
                     DMNQALRERTVERLKNGKLDIVVATDVAARGIDVPRISHVINYDIPYDTESYVHRIGR
                     TGRAGRNGKAILFVAPREQRMLQSIERATRQPITMMQLPSKEQITHHRINAFKQSIVD
                     VLNTENLDFFSTLVAELCKEQQILPEDAAKALAFLAQKGNPLLIERKESEPEGDFKRE
                     RPERGEKPARGERKERGERREFTSDVPMTTYRIEVGREHGVMPKDIVGAIANEVQLNR
                     DHIGHIRLFELFSTVELPTDLPRELIAHLKKVRVKSQRLDITVDTGPGKKSFEGGKFD
                     KPKADKPKFNMFDDSKEERKPKRKAAAEAPAGKKPSPKAAEAETGKSPKGKRRSLSSS
                     DWD"
     gene            complement(2831..>2937)
                     /locus_tag="PKZ52_15785"
     CDS             complement(2831..>2937)
                     /locus_tag="PKZ52_15785"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNG61283.1"
                     /translation="WQTYVDGQTFEVEANVRFKVKALTDTAYLCKYWR"
BASE COUNT          608 a          707 c          851 g          771 t
ORIGIN      
        1 ataaaaggct ttaactctat gcctcccaaa ggaacctgcg ccgattgtag cgacgacgaa
       61 attaaaggcg cggtgaaata tatggtggat aaagccaaat aataacgccc tggtagaaaa
      121 ggccgcacag cggccttttt ttgtgtctgt gtatcggcga taagggcatc aggtcgtgac
      181 cggtggctca acccagtgca aacccggcaa acccaggtct caatttgtgc tgtcatctgg
      241 gcaggatggg caaccagctc ctaaacttgg ggtaaaccgc tgaccctgcg gcaaaccacc
      301 ggctttgtga gacctgtatg actgtttact gttattccaa aaccaaacag gcggtgctgt
      361 atcttttgct ggcctgcctg cccgtggtgg ggctgatgtg tttttatctg gcgctcaata
      421 tgtgggaggc cgcccaatgg ttttgggtcg ggctctgggg gatgatcagt gcggtatttc
      481 tcggcttctt ttttgttggg ctgcgaatgc gcagctttat tcatacaggt attgcgttaa
      541 ccgaggaggg tttggaattt catcagggtg cggttaataa gctgcacccc tggagtgaag
      601 tgggcgcggt aaaaagctgg ccctggattc aattgttggt ggtgtttgat gctaagggta
      661 gggtgttgtt accggtggat catatgctga ccaacttccc tgcttttaaa caggcccttg
      721 aggcgcgact tacacaaccg gctgtggcca gcgagattgt gtaggccgca agacttactc
      781 taatcccaat cgcttgaact gagtgaacgg cgtttgccct tagggctttt gccggtttcg
      841 gcctcagcag ctttagggct gggctttttc cctgccggtg cctcagcggc ggctttgcgc
      901 ttaggtttgc gctcttcttt gctgtcgtcg aacatattga atttaggctt atccgctttg
      961 ggcttatcga atttgccgcc ctcaaaggat tttttgcccg gccctgtatc caccgtaata
     1021 tccaagcgct gggacttgac gcggactttt ttcaggtggg caattaattc ccgcggtaaa
     1081 tcggtgggca attcaacggt gctgaataat tcaaataggc gaatatggcc tatgtgatcc
     1141 cggttgagtt gcacctcgtt ggcgatggca ccgactatgt cttttggcat cacgccatgc
     1201 tcacggccca cttcaatgcg gtaggtggtc atgggcacgt cgctggtaaa ttcccgccgc
     1261 tcgccgcgct ctttgcgttc accacgggcg ggcttctcgc cgcgctctgg gcgctcccgt
     1321 ttgaaatcac cctctggttc gctctctttg cgctcaatta acagcgggtt acccttttga
     1381 gctaaaaagg ccagggcctt ggcggcgtct tcagggagga tttgttgttc cttgcacagc
     1441 tcggccacca gggtggagaa aaagtctaaa ttttccgtat ttaacacatc aacgatggac
     1501 tgtttaaacg cgttaatcct gtgatgggta atctgctctt tgctgggcag ctgcatcatg
     1561 gtgatgggct ggcgggtggc gcgctcgatg gactgcagca tgcgctgctc gcggggggcc
     1621 acgaataaaa tagccttacc gttgcggcct gcgcggccgg tgcggccgat gcggtgcacg
     1681 taggattcag tatcgtaggg aatgtcgtag ttaatcacgt ggctgatgcg gggtacgtcg
     1741 ataccacgag cggcaacgtc ggtggccact actatgtcca gcttgccatt ttttaggcgc
     1801 tcaaccgtgc gctcgcgcag ggcttggttc atatcgccgt tcagggcgga ggtggcatgg
     1861 ccgcgggctt cgagtttttc ggctaattcc accgtagcgg ttttcgtgcg cacaaaaata
     1921 attacgccgt cgtattcttc aatttccaaa atacgggtga gggcgtccag tttatttacg
     1981 cctttaacaa tccagccctg ttgatcgata ttcatcaccg tggtggtttt gctttcgatc
     2041 tgaatagtga cgggattatt taagtaggtc tgcgcgactt tttgaatttg tttgggcatg
     2101 gtggcggaaa atagcgccgt ctgccgatct gttggcacct gctcaagaat ccactcaaca
     2161 tcatcaataa accccatgcg cagcatttca tcggcttcat ctagcaccac gtgggtgatg
     2221 ccgtctaaat ccaacgattt acggcgcagg tgatccataa cgcggccggg ggtgcccacc
     2281 actatgtgca cgccgcgttc aagggcgcgc agctggctca acatgctttg gccgccataa
     2341 ataggcagca cgtggaaatc ttttaaattg tgggcgtatt tttgaaaggc ctccgctact
     2401 tgaatggcca gttcccgcgt aggggtaagg attaaggctt gggtgtggcg cttttttaaa
     2461 tccatggcgt taagcactgg cagcgcaaaa gcggctgttt taccggtacc ggtttgcgcc
     2521 atacccaata catcgtggcc gttgagtagg ctggggatgg cttgcgcttg gatgggggag
     2581 ggctgttcgt agccgatttc tttaagtgcg gagagaaggg tgggtttgag agcgaagtcg
     2641 gaaaattggg tgatatctgt cataaataaa ccaaaagccg ccgcagcctt ctgggtgcgg
     2701 aggtggtgaa aagggcgcgc gattgtacag cgagactggt caaaaataaa gcaataaggg
     2761 cttcgctggc ggcaaaaccc taaacaagcg cggcaagtcg ctgatgagac ttgccagtac
     2821 ggggaatagg ctagcgccag tatttacaga ggtaggcggt atcggtaagg gcctttactt
     2881 taaagcgcac attggcttcc acctcaaagg tttggccatc tacataggtt tgccagc
//
LOCUS       DAOQGS010000532         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20424_length_2935_cov_5.096875, whole genome shotgun sequence.
ACCESSION   DAOQGS010000532 DAOQGS010000000
VERSION     DAOQGS010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20424_length_2935_cov_5.096875"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PKZ52_15790"
     CDS             complement(<1..162)
                     /locus_tag="PKZ52_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61284.1"
                     /translation="MFIAKPRSAFNNPHRCLMVQGTTSDAGKTTLVAGLCRLFARQGL
                     SVAPFKPQNM"
     gene            155..1096
                     /locus_tag="PKZ52_15795"
     CDS             155..1096
                     /locus_tag="PKZ52_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNG61285.1"
                     /translation="MNIRSNLINLGRAVIAALLLLLIVTHAHRATAGAQISAVDARGK
                     TLIFTKRPERIISLAPSITELLFSAGAETALVGTVDFSDYPAAALKIPRVGSYASFDY
                     EAIVKLQPDLIIGWQSGNPAEKIAHLERLGLRVLLIEPHRLADLIASVHTFAQLAGNS
                     AQAQASIDAFNQIVTPWQKGAKPTVPVRVFYQIWHQPLMTLNKEHLFNELLTTCGGEN
                     VFGDLAPLAPKIDIEAVLKANPDLIIASGMDTARPQWLDQWHQWPQLKAVKNGHLFFI
                     PPDLLQRQSLRAADGLQQLCTAIDKATRKSDGEKATR"
     gene            1792..2145
                     /locus_tag="PKZ52_15800"
     CDS             1792..2145
                     /locus_tag="PKZ52_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNG61286.1"
                     /translation="MSLFERIGGAPAVDAAVNIFYRKVLGDERISHFFESVDMERQAA
                     KQKAFLTMVFGGPNQYTGLDMRKAHAGMHLTEEHFDAVVQHLGSTLTQLGVAADDIAQ
                     IAALAQSVKADVLNQ"
     gene            2147..>2935
                     /locus_tag="PKZ52_15805"
     CDS             2147..>2935
                     /locus_tag="PKZ52_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNG61287.1"
                     /translation="MARVEFNRQVFELADGSNLLSALEQRGLNPRRGCGVGVCQGCLV
                     QILEGIISPRAQEGLSPHQTAQGLAMACMCQVSGIFRAQTLSEPSTRFASEVLRQRWL
                     KGDILQLCVSKPFDYQPGQFVRIFATEGAVGELNAVGRCYSLASLPQEAHLEFHIRVL
                     PDGQMSQYLAHIPLGSPLYLQGPMGRCFYQATSLGQPLLLAAIGTGMAPILGVARAAL
                     KAGHTGPIYCVMGAKEESGLYPLPGLHQVAAAPNVKISYIVQQAG"
BASE COUNT          703 a          791 c          748 g          693 t
ORIGIN      
        1 catgttttgc ggcttaaacg gcgccacaga caatccctgc cgcgcaaata gccgacacaa
       61 ccccgccact aaagtggttt tacccgcatc cgaggtggtg ccctgcacca ttaaacaacg
      121 gtgtggatta ttaaacgcgc tacgaggctt ggcgatgaac attcgaagta atctcattaa
      181 tttagggcgc gctgttattg ccgcactttt gttattgcta attgttacac acgcccatag
      241 ggcaactgcg ggggcgcaga ttagcgcagt ggatgcacgg ggcaaaaccc ttattttcac
      301 taaacgcccc gagcgcatta tcagccttgc accgagcatt accgaattat tattctccgc
      361 cggtgccgag accgccctcg tcggcacagt ggatttcagc gactaccccg cagcggctct
      421 gaaaattccc cgcgtgggta gctacgcgag ttttgattac gaggctattg taaagctaca
      481 accggattta attatcggct ggcaaagcgg caatcccgca gagaaaattg cccatttgga
      541 gcgtttaggg cttagggtat tgttaataga gccccaccgt ttagcggatt taattgccag
      601 tgtgcacacc ttcgcgcagt tggctggcaa tagcgcccaa gcccaggcca gtattgatgc
      661 ctttaaccaa attgtgacgc cctggcaaaa gggcgctaaa ccaactgtgc cggtgagggt
      721 gttttatcaa atatggcatc agccgttaat gacactgaat aaagaacatt tatttaatga
      781 gttgctgacc acctgcggcg gggaaaatgt gtttggcgac ttagcacccc tagcccccaa
      841 aattgatatc gaagcggttt taaaagccaa tccggacctt attatcgcca gcggcatgga
      901 taccgcgcgg ccccaatggc tggatcagtg gcaccaatgg ccacagttaa aggcagtaaa
      961 aaacggccat ttatttttta ttccccctga tttattgcag cgccagagct tgcgtgctgc
     1021 tgatggattg cagcagttat gcacggcaat tgacaaagca actcgcaaga gtgatggtga
     1081 aaaagcaact cgttaaggtg gtgctaaaaa atcaactcct aaaacgcgct gtgcaaatga
     1141 aacctgtcag agactggcct gaaaacattc gcttaacaag cggcgatata aaacctgcaa
     1201 tgatcctcag cagcgactaa attccctgct tggcagtaag tacctggcag cacccgcacc
     1261 gtttgcacag caacatagat gcacatttaa gaaacctctg aaaaattacc ttgctcgctc
     1321 tggcggcgtt gaatttgggc taggcgcccc cgcagaaata ctcatttact gcatgtaaac
     1381 tccgcttttt ttgccggggg cgcccagcca aattcgccta gccagagctt cgccgccatc
     1441 atctttcata ggttccttca gtaagtcggc aggcaaccca tttctcccac ctatttcaaa
     1501 tcagcataag cttgcagatt gcccgctctg ggcactgctc acgaagggct aaataattaa
     1561 tttttccgca gcgcccgctc tttagtcgat tcaatctgca cctcaacaca aatttgattt
     1621 gggtcaggca aacctcaaaa gacgctaaac ccctcactta aacctgctag gcttgcctcc
     1681 agtagggttg ttaaacccgc ccgtcgtcgc ctgcatccgc tgccgtgtac gcagttttca
     1741 catcattgct gcgggtgatt attttcctca atcattaaaa gagttgtcgc tatgagccta
     1801 tttgaacgca ttggcggtgc gcccgctgtg gatgccgctg tgaatatttt ctatcgcaaa
     1861 gtattgggcg atgagcgcat tagccatttt tttgaaagcg tggatatgga gcgccaagcg
     1921 gccaaacaaa aagctttttt aaccatggtg tttggcggcc ccaaccaata caccggcctg
     1981 gatatgcgca aggcccatgc tgggatgcat ctcaccgaag aacattttga tgcggtggtt
     2041 caacacctag gcagcaccct aactcaattg ggcgtggcgg ccgacgacat agcgcaaatc
     2101 gctgctttag cgcaaagcgt taaggcagat gtgctcaacc agtaatatgg ctcgggttga
     2161 atttaaccgg caggtgtttg aacttgccga tggcagcaac ctgttaagcg ccttagagca
     2221 gcgcggcctc aacccgcgcc ggggctgcgg ggtaggcgtt tgccaaggct gcttagtgca
     2281 aattttagag ggaatcatca gcccccgcgc ccaggaggga ctctcgcccc atcaaacggc
     2341 acagggcctg gccatggcgt gtatgtgcca ggtcagtggt attttcaggg cgcaaactct
     2401 ctcagagccc tctactcgtt ttgccagcga ggtgctgcgc caacgctggt taaaaggcga
     2461 tattttgcag ctctgcgtca gtaaaccgtt tgattaccag ccaggacaat ttgtgcgcat
     2521 cttcgccact gaaggggcgg ttggtgaatt aaacgcggtg ggccgctgtt attccctggc
     2581 cagtctgccc caagaggccc atttggaatt ccatattcgt gtgctgccag acggccaaat
     2641 gagccagtac ctggcgcata ttccgctcgg cagcccgctg tatctgcaag ggcctatggg
     2701 gcgctgtttt taccaagcca caagccttgg gcaaccgcta ttactggccg ccataggtac
     2761 aggcatggcg ccgattttgg gcgtcgcccg cgcagccctt aaagcgggcc acacggggcc
     2821 gatttattgc gtgatgggcg ccaaagagga gagcggcctt taccccctgc cgggtttaca
     2881 ccaggtggca gcggcgccca atgtgaaaat ttcctacata gtccagcagg cgggc
//
LOCUS       DAOQGS010000533         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20547_length_2925_cov_4.477003, whole genome shotgun sequence.
ACCESSION   DAOQGS010000533 DAOQGS010000000
VERSION     DAOQGS010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20547_length_2925_cov_4.477003"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKZ52_15810"
     CDS             complement(<1..322)
                     /locus_tag="PKZ52_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNG61288.1"
                     /translation="MQPSQFSVAAPRKIHLHQKSQQLELVYADAQFFLPAEYLRVFSP
                     SAEVQGHGPGQQVLQVGKMHAGIKAIEGQGNYAIKIVFDDGHDSGIYTWNYLYELGQF
                     QAKNW"
     gene            complement(369..1700)
                     /gene="hslU"
                     /locus_tag="PKZ52_15815"
     CDS             complement(369..1700)
                     /gene="hslU"
                     /locus_tag="PKZ52_15815"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNG61289.1"
                     /translation="MAHMTPREIVAELDKHIVGQQAAKRAVAIALRNRYRRMQISGSL
                     RDEITPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LVDSSIKMLREQTKQKMLAKAQDAAEERILDALLPPPRSGFGGAEGEEKHESSTRQLF
                     RKKLREGALDDKEIELDVAAASVGVEIMAPPGMEDMTSQLQNMFSSIASGKTKKAKLT
                     VKQALKKLTDEEAGKLINEEELKARAIEAAEQNGIVFIDEIDKVAKRQSNSGADVSRE
                     GVQRDLLPLIEGCTVTTKHGSIKTDHILFITSGAFHLTKPSDLIPELQGRLPIRVELE
                     ALTPADLERILTEPDHSLTTQHKALLATEGVELNFTADGIRRIAEIAHQVNETTENIG
                     ARRLHTVLERLLESVSFDSGVEQTALQVTAEFVDQELGNLAKNEDLSRYIL"
     gene            complement(1748..2287)
                     /gene="hslV"
                     /locus_tag="PKZ52_15820"
     CDS             complement(1748..2287)
                     /gene="hslV"
                     /locus_tag="PKZ52_15820"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNG61290.1"
                     /translation="MEQYRGTTILSVRRNGRVVIGGDGQVSLGNTIMKGNARKVRRLY
                     KNQVIAGFAGGTADAFTLFERFEAKLESHGGQLVRAAVELAKDWRTDRALRRLEALLA
                     VANAEASLIITGNGDVIQPEQDLIAIGSGGSFALSAARALYNNTDMQARDIVEKSLTI
                     AGDICVYTNHQFTIEELEY"
     gene            complement(2372..>2925)
                     /locus_tag="PKZ52_15825"
     CDS             complement(2372..>2925)
                     /locus_tag="PKZ52_15825"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNG61291.1"
                     /translation="GAEPKRQPKPPVPGWIWLITGCLLGAFVMFLLHVSKIPPAPPHT
                     DKPAPQAASAEKGPAEAKPEADDSVKPRYDFYRLLKENQPPHSPRSETPVSTNPNVPV
                     DANANFVLQVASYKTAEDAEATKAKLLLIGLTARVEKVTLRNTETWHRVLIGPVKSVS
                     EADRIRGQLTDNKYDALVLKVAP"
BASE COUNT          592 a          718 c          856 g          759 t
ORIGIN      
        1 gccaattctt ggcctgaaat tgccccagtt catacagata attccaggta taaatacccg
       61 agtcgtggcc gtcgtcaaag acaattttaa tggcgtaatt gccttggcct tcaatagctt
      121 ttatgcccgc atgcattttg ccaacctgta aaacctgctg ccccggcccg tggccttgca
      181 cttcggcgga aggggaaaat acccgtaaat attccgccgg taggaaaaac tgtgcatcgg
      241 cgtaaaccaa ttccaactgc tgagattttt gatgcaggtg aattttgcgg ggcgcggcaa
      301 cggagaattg agacggctgc ataatcgtgt cctcaataaa aaagcccagg gacactgggc
      361 tttttaggtt aaagaatata gcggctgaga tcctcgtttt tcgctaagtt gcccagctct
      421 tgatcgacaa attcggcggt aacctgcagg gcggtttgct ccacacctga gtcaaaggag
      481 acagattcca acaaacgctc cagcaccgtg tgcaaacggc gggcgcctat attttctgtg
      541 gtttcgttta cctggtgggc aatttcggcg atgcggcgaa tcccgtcggc agtgaaattt
      601 aattccacgc cttcggtggc caacagcgct ttatgttgcg tggtaaggga gtgatccggt
      661 tcggtgagaa tacgctctaa atcggcgggg gtgagggctt ctaactccac gcggataggc
      721 aggcggccct gcaattccgg gattaaatcc gagggtttgg ttaaatgaaa agcgccggag
      781 gtgataaata aaatatggtc ggttttgatg gagccgtgtt tggtggtgac ggtgcagcct
      841 tcaattaagg gcaataaatc ccgctgcaca ccctcgcggg agacatccgc gccggaattg
      901 ctttggcgtt tggccacctt gtcaatttcg tcgataaaga caataccgtt ttgttctgct
      961 gcctcaatag cccgcgcttt taattcctcc tcgttaatca gcttgcccgc ttcttcatcg
     1021 gtgagttttt tcagcgcttg tttaacggtt aatttggctt ttttggtttt gcctgatgca
     1081 atagaggaaa acatattttg taactggctg gtcatatcct ccatgcccgg cggcgccata
     1141 atttctacac ctacggaggc cgcagctaca tctaattcaa tttctttatc atccagagcg
     1201 ccttcgcgca attttttgcg gaataactgc cgggtggagg attcgtgttt ttcctcgccc
     1261 tcagcgccgc caaaaccgct gcgcggcggt ggtaacaaag catccaaaat gcgctcttcg
     1321 gcggcatcct gggctttggc gagcattttt tgtttggttt gttcgcgcag cattttaatg
     1381 gagctatcca ctaaatcgcg aataatggaa tccacatcgc ggcccacata acccacttcg
     1441 gtgaatttgg tggcttccac tttaataaat ggcgcattgg caagttttgc taagcggcgg
     1501 gcaatttcgg ttttgccaac gcctgtgggg ccgatcatta aaatattttt gggggtaatt
     1561 tcatctctca ggctgccgct aatttgcatg cggcggtagc ggttgcgcag ggcaatggcc
     1621 accgcccgtt ttgcagcctg ttggccgact atgtgtttat cgagttcggc gacgatttcg
     1681 cgaggagtca tgtgtgccat ggcaggtctc aaggttaatg cggtcaataa aaggcagctg
     1741 gcgagaatta atattccagc tcttcaatgg tgaactggtg gttggtgtac acacagatgt
     1801 cgccagcgat ggtgaggctt ttttccacta tgtcgcgagc ctgcatatcc gtgttgttgt
     1861 agagggcgcg ggcggcggag agggcgaagg agccgccgga gcctatggca attaaatcct
     1921 gttccggctg gatgacatcg ccgttgccgg taatgatgag ggaggcctcg gcattggcaa
     1981 ccgctaagag cgcctctaag cggcgcagag cgcggtcggt gcgccagtct ttggccagtt
     2041 caacggcagc gcgcaccagt tggccgccgt ggctttcaag cttcgcttca aagcgctcaa
     2101 acagggtaaa ggcgtcggcg gtgccgccag cgaagcctgc aatcacttgg tttttataca
     2161 ggcggcgcac tttgcgcgca ttgcccttca tgatggtgtt gccgagggag acctggccgt
     2221 cgccgccgat caccacacgg ccattgcgcc gcaccgataa aatggttgtt ccccgatatt
     2281 gttccacgcc tgcctcttgc tactgttgct gtgcataagg cgaggttagt gggggcgcgg
     2341 gaggttattt caagccttgg gcgggcaggt gctagggggc gacttttagc accagggcgt
     2401 cgtatttgtt gtcggtgagc tggccgcgaa tacggtcggc ttcgctgacg gatttaaccg
     2461 ggccgatcaa tacgcggtgc caggtttctg tgttgcgcag ggtgactttt tccactcggg
     2521 cggtaaggcc gatcaacaac aatttcgctt tggtagcttc ggcatcctcg gcggttttat
     2581 agctggctac ttgcagcaca aagttggcgt tagcatccac tggcacattg gggttggtgc
     2641 tcaccggggt ttcgctgcgg gggctgtggg gcggttggtt ttctttgagc agacggtaaa
     2701 agtcatagcg tggtttaacg ctgtcgtcgg cctcgggctt ggcctcagca gggccttttt
     2761 cggcgctcgc cgcctggggg gcgggtttat cggtatgggg cggggcggga ggaatttttg
     2821 agacatgcag gaggaacatt acaaaggccc ccaataaaca gccggtaatt aaccaaatcc
     2881 agccgggcac tggcggtttg ggttggcgtt tgggttcggc gccgt
//
LOCUS       DAOQGS010000534         2921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20608_length_2921_cov_4.138870, whole genome shotgun sequence.
ACCESSION   DAOQGS010000534 DAOQGS010000000
VERSION     DAOQGS010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20608_length_2921_cov_4.138870"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2780
                     /locus_tag="PKZ52_15830"
     CDS             <1..2780
                     /locus_tag="PKZ52_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG61292.1"
                     /translation="FAGTLLLGLYPFAKPSFLNLPVNADMVHTVFITGFIPLFFAFMV
                     KAFEKRQHFIAKLSERMWLANKAVGLGVWEWSISTRNVWWDSAMYDLYGLAQQPMTYA
                     RWRQCIHPDDVNQLERLLKSMARAKNEFSTSFRIIHPVLGIRHIQAAATIVYGAANRP
                     LRYVGMNWDVTNLKSAELALHQAKQKAEVASHTKSEFVANMSHEIRTPLNATLGAAQL
                     LQTTELSAPQKKYINMIKHSGESLLAIVNDILDFSKIESGHAQLAMNIFNLRESVQKI
                     ATILSPQAGAKGLEFILHIDKNVPTCVEGDALKLHQVLVNLTSNAIRFTPGGEVTIEI
                     TANKPKHGRVQVGFAVTDTGIGIAKNNQLNLFDAFTQVDSSSTRQYGGTGLGLAIAKR
                     LVNLMGGELLLESEEGLGSRFYFSLEFTQIASRNYTHPLEHQPLRVLLVDHNSRVIDA
                     VKHLVAHWPWDIIAAPSVQQALNIFPTANKLKPIDLLIIDSRVLNEDVLFIQQLSKLG
                     LEPNCLRLVSDINHAAIALPAHCLDLVDAYLIKPITHAGLLDAITDGRTLRQGSHKPL
                     QQEMNLSNRLLSGINILLVEDNPLNQVVAKGLLEQFGAHIDQAWNGKEALELISHNPQ
                     QYHLIMLDIQMPIMDGYSTCHALNERGNKIPIIAMTAGVLSSEREHCERMGMQGFVPK
                     PIEMQTLLNEIQRLLPKLEAAKTSAPLDITVKESQPIAHTLFYPERLLHHINQPAQIN
                     SVLQGIQQLIDSSAAQQTALIHSIEEADFSAAAKILHSQKGAWGNLGATPLYRQICSL
                     ETLAQENRLSLNCRQWLAYQQLYAQTRKAAEHWLQHYYTNETSHTRAPAAAELNTAGV
                     WVENFLQLLDEHSLRACDQYEQLKTHFSAHLDAESAHQLDNAMHNLHFPLAAQILREQ
                     RVNQTAFDTTL"
BASE COUNT          791 a          766 c          674 g          690 t
ORIGIN      
        1 ggtttgcggg tactctgctg ctggggcttt acccctttgc caaaccatct tttttaaatc
       61 tgccggtcaa tgccgacatg gtgcataccg tatttatcac cggttttatt ccgctttttt
      121 ttgcgtttat ggtaaaggcc tttgaaaagc gccaacactt cattgccaag ctgtccgaac
      181 gcatgtggct tgccaataaa gccgttggtt tgggtgtgtg ggagtggagt attagcaccc
      241 gcaatgtttg gtgggatagc gccatgtacg acttgtacgg cttagcccaa cagccgatga
      301 cctatgcgcg ctggcggcaa tgtattcacc cggatgatgt caaccaactt gagcgcttgc
      361 ttaaaagcat ggcgcgggca aaaaatgaat tttccacctc ctttagaatt attcacccag
      421 tattgggcat acgccatatt caagccgccg ccaccattgt gtacggcgcc gccaaccgcc
      481 ccctgcgcta tgtgggcatg aattgggatg tgaccaattt aaaatccgcc gaattagccc
      541 tgcatcaagc caaacaaaaa gcggaagtgg ccagccacac caaaagtgaa tttgtggcca
      601 atatgagcca tgaaatccgc acaccgctca acgctaccct aggtgccgca cagctactac
      661 aaaccactga actatccgca ccgcagaaaa aatacatcaa tatgatcaaa cactcagggg
      721 agagcttgct ggccatagtg aatgatattt tagatttctc caagatcgaa tccggccacg
      781 cccagctggc catgaatatt tttaacctgc gggagagcgt gcaaaaaatt gccactattt
      841 tatcgccaca agccggtgca aaaggcttag agtttatttt acatattgat aaaaatgtac
      901 ccacctgtgt tgaaggcgac gcactcaaac tgcaccaggt attggtgaac ctaacctcca
      961 acgctattcg ttttacgccc ggcggcgagg taaccattga aatcactgcc aacaaaccca
     1021 aacacggcag ggtacaggta ggttttgcag tgacggatac cggcataggc atagccaaaa
     1081 ataatcaatt aaatttattt gatgctttta cccaggtaga ttcttcctcc acgcgccaat
     1141 atggtggcac agggttgggc cttgccattg caaaacgctt ggtgaattta atgggtgggg
     1201 aattgctgct ggagagcgaa gagggtttgg gctccaggtt ttatttctcc cttgaattta
     1261 cccaaatcgc ctcgcgcaat tacacccatc cactcgaaca tcaaccccta cgggttttat
     1321 tggtggatca caacagccgg gtgattgacg cggtcaaaca tctggtagcc cattggccct
     1381 gggatattat tgccgccccc tcagtgcaac aggccttgaa tattttccct acggctaaca
     1441 aattaaaacc catcgattta ttaattattg atagccgcgt attaaacgaa gatgtgttat
     1501 ttattcagca gctgagtaaa ctcggccttg aacctaattg tctgcgcttg gtaagcgata
     1561 taaaccatgc cgccatcgcc ctgcccgctc actgcttgga tttagtagat gcctatttaa
     1621 ttaaaccgat tactcacgca ggcttattag acgccattac agatggccgc accctacgcc
     1681 aaggcagcca caaaccgctg caacaggaaa tgaatttaag caaccgcttg ctcagcggca
     1741 ttaatatttt gctggtggaa gataacccgc tcaatcaggt agttgccaag ggtttgctgg
     1801 agcagtttgg cgcccatatt gatcaggcct ggaatggcaa agaagcctta gaattaataa
     1861 gccacaaccc ccagcagtac catttaatta tgctggatat tcaaatgccc ataatggacg
     1921 gttactccac ctgccatgca cttaatgagc gcggcaataa aattccgatt atcgctatga
     1981 ccgcaggtgt tttatccagc gaacgggaac attgtgagcg catgggtatg cagggttttg
     2041 tgcctaaacc cattgaaatg caaaccctgc tcaatgagat tcaacgcttg ttgcctaagc
     2101 ttgaagccgc taaaacaagc gcaccgctcg acattaccgt taaagaatcc caaccgattg
     2161 cacacacctt attttatcct gagcgcctgc tgcaccatat caatcagccg gcacaaatca
     2221 atagcgtgct gcagggcatt caacagctga ttgacagcag cgcagcccaa caaaccgcgt
     2281 tgattcacag catcgaagaa gcagacttta gtgcagcggc gaaaatttta cacagtcaaa
     2341 aaggcgcctg gggaaaccta ggtgctaccc ccctgtaccg gcaaatctgt agtttagaaa
     2401 ctctcgcgca ggaaaatcgg ctgtccctta attgccgcca atggttggcc tatcaacagt
     2461 tgtatgcgca aacgcgcaaa gcagctgagc attggctgca gcactattac accaatgaaa
     2521 caagtcacac ccgtgcacct gctgccgccg agctaaatac cgccggggta tgggttgaga
     2581 actttcttca gctgctggat gagcacagcc tgcgcgcctg cgaccagtac gagcagctaa
     2641 aaacccactt tagcgcccac ttggacgccg agagcgcaca tcagttggat aacgcaatgc
     2701 acaacttgca ctttccactg gcggcgcaaa tactgcggga gcagagggtg aaccaaacgg
     2761 cctttgacac cacgctttag aaggctgtta ttaacccggc agaatttaga gccgggttaa
     2821 taatggcggt aaggctcgcg acacaccgcc ggaagctgcg ctaaaacacg aaattccgcg
     2881 cattctcgca agtgccagcc cagcgcaggc taaattgatc a
//
LOCUS       DAOQGS010000535         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20622_length_2920_cov_4.306108, whole genome shotgun sequence.
ACCESSION   DAOQGS010000535 DAOQGS010000000
VERSION     DAOQGS010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20622_length_2920_cov_4.306108"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1743)
                     /locus_tag="PKZ52_15835"
     CDS             complement(181..1743)
                     /locus_tag="PKZ52_15835"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNG61293.1"
                     /translation="MVFSNLTGDYKTLRYAVAFTALITSALTGCTGGGTSSSSSSSSS
                     SSSSSSSSNSSSSSSSSSSSSSSSSSSSSSSSSSSSGGISPNDKLVIAINAGSTSAAT
                     LDGVEYKADRFASAGTSQVVTDAIVGTNEDTLYQSERYGTFHYDIPVTNATYTVVMHF
                     AELYHSSAGARAFDVAVEGQTKLSNVDLFSLVGKDAAYKYVVQNVVVNDKTLTIDLSS
                     TIDNATISGFAIYSNNGGAYEEPPEPVCSTADRQTPTAIDYNRAVTRTEQRNPPSGAY
                     GYAIEHATQTLPNHTIYRPDLSKVTKIPIVAWGNGACSNVGTEQADFLLQIASNGYLV
                     IANGGPFGSGSNDQHETELVKAINWAVAENSRKCSQYYGKIDVEKVATMGWSCGGGMA
                     HFAAVDPRVDTAVALNSGLGIYSDRQSYYPRFHSPLAIFNGNSSDVAYNPGMQEYSEV
                     TNVPVYHANYPQGHGDAYFQDNGGEFGIVAVGWLNFMLKDDMGATGKGMFFGNNCRLC
                     KSPWVAKSKGFN"
     gene            2475..>2920
                     /locus_tag="PKZ52_15840"
     CDS             2475..>2920
                     /locus_tag="PKZ52_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61294.1"
                     /translation="MNDTLPGLTRRTLLVGSAAAATSWQGLAFAAAASGIAASVNTQA
                     LGPVISPKIYGGFIEHIGNLINHSLWSEMLDDRKFFHGVIEKPETKPTHPLAALSFNQ
                     KWLAVGPITAIQLDTNTVWVGQHSPVITASSAEPIGMAQQDLALKAT"
BASE COUNT          733 a          668 c          734 g          785 t
ORIGIN      
        1 tactgcgtgt aaactcggca attgctcctg cattgctcta cctcctacct ccctgtagtc
       61 gtccgctttt tttggcaaat tcgcctagcc agagattcgc cgccgacatt tttcagaggt
      121 tccttaaaaa taaaaaaggg ctgcgtatgc agcccttcgc aaataatttt tcagttaggt
      181 ttagttaaat cctttagatt ttgccaccca aggtgattta cacaagcggc agttattacc
      241 aaagaacatg cctttacctg tagcgcccat gtcgtctttt aacatgaagt tcaaccagcc
      301 tacagccaca ataccgaatt caccgccatt atcttggaag taggcatcac cgtggccctg
      361 agggtagtta gcatggtaaa caggtacgtt ggtgacttca ctgtattctt gcatgcctgg
      421 gttgtaggct acgtcactgg agttgccgtt aaaaatagcc aggggtgagt gaaagcgggg
      481 gtaataactt tggcggtcgc tataaatacc caggccacta tttaatgcca ccgctgtatc
      541 tacgcgcggg tcaactgcag caaagtgcgc cataccgccg ccacaggacc agcccatagt
      601 ggccactttt tccacatcaa ttttgccgta gtactgactg catttgcggc tgttttccgc
      661 cacggcccaa ttgatggctt tgactaattc tgtttcgtgt tgatcgttgc tgcctgagcc
      721 aaatgggccg ccgttggcaa ttactaaata gccatttgag gcaatttgca aaaggaaatc
      781 cgcttgctct gtgcccacat tggaacaggc gccgttaccc caggccacaa tgggaatttt
      841 ggtcactttg gataagtccg gccggtaaat ggtgtggtta ggcagggttt gggttgcgtg
      901 ttctatggca taaccatagg cccctgaagg tggattgcgc tgttcggtgc gggttaccgc
      961 gcggttgtaa tcgattgctg tcggggtttg gcggtcagcg gtagagcaga cgggctctgg
     1021 cggttcttca taagcgccgc cattattcga ataaatagca aagccgctaa tagtggcgtt
     1081 gtcgatggtg gagctgaggt caatggtgag ggttttgtcg tttaccacca cattctgcac
     1141 cacgtattta taagcggcat ccttgccgac caaactaaat aaatccacat tactcagttt
     1201 ggtctgccct tcaacagcca catcaaacgc gcgagcgcct gcgctgctgt ggtacagctc
     1261 ggcaaaatgc atcaccacgg tataggtggc attggttacc ggaatgtcgt agtggaaggt
     1321 gccatagcgc tcagattggt aaagcgtatc ttcattggtg ccgacaatcg catccgtcac
     1381 cacctgggat gtgcccgccg aagcgaaacg gtcagcttta tattcaacac cgtccaaggt
     1441 tgccgctgag gtgctgccgg cattaatggc gatgaccaat ttatcattcg gtgaaattcc
     1501 gccagagctt gagctgctgg agcttgacga gctgctggaa gaggaactgc tggagcttga
     1561 tgaactagac gagctagagg aggaattact ggagcttgat gaactgctcg aactggaaga
     1621 tgagctactg gaggacgagg tgccgccacc ggtacatcct gttagcgcag aggtaattaa
     1681 agcggtaaat gccactgcat accgcaaggt tttgtaatcg cctgttaagt ttgagaaaac
     1741 catacgaagg tgccttttta tagtggagtc cggttgtgat gcagaatttt ttgcttcgct
     1801 tatagagctg ttgcatcatt agggcatatc atatgactaa atgatgtggg cgtgtgtgcg
     1861 ttacttcata ttggcaatgg ggaaatcgtt ttaataatga ttaatcccgg caattatgtg
     1921 aggtgggttt tgtgcagtct gttattcaag gcaatacttt ttgcgcggca atcaggccta
     1981 gcctaaaagt cagttttacc ttattgcggt gggcatacag ccgttaccta tgcggccagt
     2041 ttattttcat aattattggc tgaattgtga gcgaggattc catcgcgctt taaagtacaa
     2101 aagagcaagt ctgagcgcct attaactcgg cggcaaaata tatcgtcctg atctattttt
     2161 tgccttcact gctctgactc ttgcgaaaat gcgcggattt tcttgtttta gcgcagctcg
     2221 tggcggggcg gcacccctgc cgccattatt aacctggcac taaatgccgc tgggttaata
     2281 aaaacttgtg aatatgccaa gccagtgctc aaagatgatt tcccactgtt aacaagtaat
     2341 tttataatcc gtgctggtag aaagcctgca ccctatatgt attacgcctt aagggtaata
     2401 ctaattgccg cagactattt ttagtcgata aattttaaaa aacaccatta ataatttaat
     2461 aagaggaggg tgaaatgaac gatacactac cgggtttaac gcgcagaact ttgctggtgg
     2521 gtagcgcagc tgctgcaacc agctggcagg gtttggcatt tgcggcggcg gcaagtggaa
     2581 tagccgccag tgtaaataca caggcgctag ggccagtaat atcgccaaaa atctacggcg
     2641 gctttattga acatataggc aacttaatta accatagcct gtggagtgaa atgcttgatg
     2701 atcgtaaatt tttccacggc gttatagaaa aacctgaaac aaaacctacc catcccttag
     2761 cggcactgag ttttaatcaa aaatggctcg ccgtaggccc catcactgcc attcaattgg
     2821 atacaaacac cgtatgggtg ggccagcaca gcccagtaat taccgcgagc agtgctgagc
     2881 ctataggtat ggcgcaacag gatttagctc taaaagcaac
//
LOCUS       DAOQGS010000536         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20636_length_2918_cov_5.626965, whole genome shotgun sequence.
ACCESSION   DAOQGS010000536 DAOQGS010000000
VERSION     DAOQGS010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20636_length_2918_cov_5.626965"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1135)
                     /gene="ychF"
                     /locus_tag="PKZ52_15845"
     CDS             complement(44..1135)
                     /gene="ychF"
                     /locus_tag="PKZ52_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNG61295.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQAGIGAENFPFCTIEPNSGVV
                     AVPDPRLDALAAIVKPERILATTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAH
                     VVRCFDDDNVIHVANGVNPAADIDVINTELALADLETIDKAIFRYAKAAKGQDKQAVA
                     MKALLEKVQPHLDQAKPLRSFGLDKDELALLKELHLLTLKPTMYIANVHEDGFENNPH
                     LDAVRAIAKAEGAMVVPICNKLESEIAELDASEKSEFLAEMGMSEPGLDRLIRAGYEL
                     LGLQTYFTAGVKEVRAWTIPIGTTAPKAAAVIHTDFEKGFIRAEVIAYNDFVQFKGEA
                     GAKEAGKWRLEGKEYIVADGDVMHFRFNV"
     gene            complement(1145..1726)
                     /gene="pth"
                     /locus_tag="PKZ52_15850"
     CDS             complement(1145..1726)
                     /gene="pth"
                     /locus_tag="PKZ52_15850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNG61296.1"
                     /translation="MPTPVQLIVGLGNPGPDYARTRHNAGADWVEQLAQLLNTPLVNT
                     PKFFGLTGRANTPQGELRLLIPTTYMNRSGQSVAALAQFYKIPVEAILVAHDELDLAP
                     GIARLKQGGGHGGHNGLRDIIASLGNNPNFMRLRLGIGHPGQAKQVADFVLKRAPSSE
                     AELTQAASEKALAVRDDLLRGDWDKALRTLHSA"
     gene            complement(1761..2354)
                     /locus_tag="PKZ52_15855"
     CDS             complement(1761..2354)
                     /locus_tag="PKZ52_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNG61297.1"
                     /translation="MSKQNFALEAHVRDVQGKGASRRLRRLNALVPAIIYGGDKAPAN
                     ISVPHKDLVKQLENEAFYSHIITLNVSGTPELVVLKGLQRHPAKPLILHADFLRVSAT
                     KKLHVNVPLHFINEETSKGVKTGGGVVSHSMTQLEILCLPADLPEYIEVDLADVEVGQ
                     IVHISDLKLPKGVESVALNHGADHDLPVCAIHKPKGE"
     gene            complement(2467..>2918)
                     /gene="prs"
                     /locus_tag="PKZ52_15860"
     CDS             complement(2467..>2918)
                     /gene="prs"
                     /locus_tag="PKZ52_15860"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNG61298.1"
                     /translation="VVVSPDIGGVVRARAVAKQLQTDLAIIDKRRPMAGVAEVMNIIG
                     EVAGRTCLLVDDMVDTAGTLCNAAVALKEHGAARVVAYCTHPVLSGPALERIVASPLD
                     ELVVTDSIPLSAAAQQEPRIRQLSLAGMLAEAMRRISNEESLSAMFR"
BASE COUNT          623 a          760 c          832 g          703 t
ORIGIN      
        1 gcacttgcaa gcatgtgcaa tcgcctgaaa tggcttgttt gaatcagacg ttaaagcgaa
       61 aatgcatcac atcgccatcg gcaacaatat attctttgcc ctccaaacgc catttacccg
      121 cttctttcgc gcccgcttca cctttaaatt gcacaaaatc gttataggca attacctcgg
      181 cgcggataaa ccctttttca aaatccgtgt gaattactgc ggcggctttg ggtgctgtgg
      241 tgccgattgg aatagtccag gcgcgcactt cttttacgcc tgcggtgaag taggtttgca
      301 ggcctaataa ttcgtaaccg gcgcgaatta aacggtccag gcccggctcg ctcataccca
      361 tttccgctaa aaattcgctt ttttcgctgg cgtctaactc ggcaatttcg ctttctaatt
      421 tattgcaaat aggtaccacc atggcgcctt ccgccttggc gatggcacgc acggcatcta
      481 aatgagggtt attttcaaaa ccatcttcgt gcacattggc gatgtacatg gtgggtttta
      541 gggtgagcag gtgtaattct tttaacaggg ccagctcatc tttatccagg ccaaacgagc
      601 gcaggggttt ggcttggtcc agatgaggtt gcactttctc aagcaaagct ttcatggcca
      661 ccgcttgttt gtcttggcct ttggcggctt tggcgtagcg aaagatggct ttgtcgatgg
      721 tttctaaatc cgccagggca agctcggtat tgatcacgtc tatgtcagcg gcagggttaa
      781 caccgttagc tacgtggata acgttgtcgt cgtcaaagca gcgcaccaca tgggcgatgg
      841 catcggtctc gcgaatattg gcgagaaact tattgcccag gccctcaccc tttgaggcgc
      901 cagcgactag gcctgcaata tccacaaact ccatggtggt ggccaagata cgctcaggtt
      961 taactatggc ggccagggcg tccaaacgcg gatctggcac ggctaccaca ccggaattgg
     1021 gctcgatggt gcagaacggg aagttctcgg cgcctatacc cgcttgggtc agggcgttaa
     1081 aaagcgtgga tttacccacg ttgggtaggc cgacaatgcc gcatttaaaa cccatgagtt
     1141 gctcctaggc tgagtgaagg gtgcgaagcg ccttatccca gtcgccgcgc aaaagatcat
     1201 cccgcaccgc cagcgctttt tcgcttgcgg cttgggtgag ttcggcttcg ctactggggg
     1261 cgcgtttgag tacaaagtct gccacttgct tggcttggcc ggggtggcca atgcctaagc
     1321 gcagacgcat aaaattcggg ttgttgccaa ggcttgcgat gatgtcccgc aagccgttgt
     1381 ggccaccgtg gccgccgccc tgtttaaggc gagcgatacc gggggctaaa tccaattcgt
     1441 cgtgggccac caaaatggct tccaccggga ttttataaaa ctgcgcgagg gccgccacac
     1501 tttggccgct acggttcata taggtggtgg gtatcagtag gcgcagctcg ccctggggcg
     1561 tattggcgcg gccggttaag ccaaagaatt tgggggtatt gaccaggggg gtattgagga
     1621 gttgggcgag ttgttccacc caatcggcac ccgcgttatg acgggtgcgg gcgtaatcgg
     1681 ggcctggatt acccaggccc acaattaact gtacaggggt tggcatagca ttacctcgtc
     1741 agccgctgta cagcgggcta ttactcgcct ttaggcttgt ggatagcaca tacgggcagg
     1801 tcgtggtctg cgccgtggtt cagagcaact gactcaacgc ccttgggcag tttaaggtca
     1861 gagatgtgca cgatttggcc aacttctaca tcggccaggt caacttcgat gtactcaggc
     1921 aggtcagcag gcaaacagag gatttccaac tgagtcatgc tatgagacac cacgccgccg
     1981 ccggttttaa cgcctttgga agtctcttcg ttgatgaagt gcagaggtac gttaacgtgc
     2041 agtttcttag tagcggacac gcgcaggaag tcagcgtgca ggattaaggg cttagcaggg
     2101 tgacgctgca aaccttttaa taccacaagc tctggagtgc cagatacgtt cagggtgatg
     2161 atgtgggaat agaaggcttc gttttccagt tgttttacca ggtccttgtg gggaacgctg
     2221 atattggctg gagctttatc accgccataa ataatggcag gtactaaagc attcaggcga
     2281 cgtaggcggc ggctcgcacc tttcccttgg acgtcacgca cgtgtgcttc taaggcaaaa
     2341 ttttgttttg acatggtttt tcttcctgcc ccgaagggcg ttttacacag ggctttcgcg
     2401 accagaagtc ctgtgggtta caaaaagccg gcgaaccggc gtggatttaa gtgctatggg
     2461 ccgctattag cggaacatgg cacttaagga ttcttcatta ctaatacggc gcatggcctc
     2521 ggccaacatg cccgcaaggc tcagctggcg aatacgcggc tcctgctggg cggcggcgct
     2581 taaggggatt gagtcggtca ccaccagttc atccaggggc gaggccacaa tgcgctcaag
     2641 agctgggccg gataacaccg ggtgagtaca gtaggccacc acacgggcgg cgccgtgttc
     2701 ttttaaagcc acggcggcat tacacagggt gcccgcggta tccaccatgt cgtccaccaa
     2761 taaacaggtg cgcccggcta cttcgccgat gatgttcatc acttcggcca cgcctgccat
     2821 gggcctgcgt ttatcgatga tggcaaggtc agtctggagc tgtttggcca ccgcacgggc
     2881 gcgcactacg ccgcctatgt cgggggatac cactacta
//
LOCUS       DAOQGS010000537         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20642_length_2918_cov_3.992665, whole genome shotgun sequence.
ACCESSION   DAOQGS010000537 DAOQGS010000000
VERSION     DAOQGS010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20642_length_2918_cov_3.992665"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /gene="argS"
                     /locus_tag="PKZ52_15865"
     CDS             complement(<1..1113)
                     /gene="argS"
                     /locus_tag="PKZ52_15865"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNG61299.1"
                     /translation="MSLRQQLAEHVRHAMNQAGVPATCDPVIAPSKNPSFGDYQANGA
                     MAAAKHMGQNPRELAQTIGAALNTGGMIAKAEVAGPGFINIHLNPAWLAQAVAEFNPS
                     QAQKVAKTQTIVVDYSGPNLAKEMHVGHLRSTIIGDAVVRVLEYLGHKVIRQNHVGDW
                     GTQFGMLLAELENQMNSGEQAELALKDLEVFYQQAKKHFDEDSAFADKAREYVVRLQS
                     GDTHLLNLWQSFRAVSLHHSEEIYQLLGVSLNASHTFGESAYNDYLAPLVANLEQQGL
                     AVNDDGAKVVFLMELADKKGNPSPVIVQKKDGGYLYATTDLAALQYRSQQLNAQRILY
                     FIDARQSLHMQQVFTLSKKAGLVPGVVQLEHHPFGTM"
     gene            1242..2027
                     /locus_tag="PKZ52_15870"
     CDS             1242..2027
                     /locus_tag="PKZ52_15870"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNG61300.1"
                     /translation="MTDIYVRSAIEAGVFYISWGNPASKNALSIAMYEQIEAALKQAE
                     AQEDIKVCVLLGGEGDFCSGNDLAEFIAGRKQGGAFFKPLLNCIHRLASFKKPLLAAV
                     NGWAVGIGATMLLHCDAVIAAPSAQFSLPFNRLGLCCECGASLLLPLRIGQGRAADWL
                     LRAEPIVAADALQAGFINRVCDDPAAVISDYAREISGRPLSALLAHRALLQEPWQEAL
                     AKAMAAEEARFFQLLQGPEFAAIVAAMSAKWRSPNPTASQPAP"
     gene            2037..2840
                     /locus_tag="PKZ52_15875"
     CDS             2037..2840
                     /locus_tag="PKZ52_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG61301.1"
                     /translation="MIQFDFTGQRVLVVGGTSGINRGIARAFAQHGAHVAVVSRSEEK
                     VQDTLAELSQFGNEVAGFTADVREYAPLSEGIKELAEAWGPLHVVVSGAAGNFPALGA
                     QMSPNAFASVVNIDLLGTYHVMRAVYPYLQRPGASVLNISAPQAQLPMLAQAHVCAAK
                     AGVEMLTRSLACEWGGEGLRVNAIIPGPIANTEGMARLAPTPELMAACAQSVPMGRLG
                     EVEDIANAALLLASPLANFITGAIVPVDGGWLQGGAAAVAASFASLLKK"
BASE COUNT          556 a          758 c          880 g          724 t
ORIGIN      
        1 catggtgcca aacgggtggt gctctaattg cactaccccg ggcaccaggc ctgctttttt
       61 gctcagggta aatacctgtt gcatatgcag gctctggcgg gcgtcgataa aatatagaat
      121 gcgctgggcg tttaattgtt ggctgcggta ctgcagggcg gctaaatccg tggtggcata
      181 taaataaccg ccgtcttttt tctgcacgat taccggggac gggttgcctt ttttatcggc
      241 caattccatt aaaaatacca ccttggcgcc gtcgtcatta actgctaaac cctgttgttc
      301 taaattggct accagggggg ctaaatagtc gttgtaggcg ctctcgccaa aggtgtggct
      361 ggcatttaac gacacgccca ataattggta aatttcttcg ctgtggtgca gtgatacggc
      421 gcggaaggat tgccacaggt ttaataaatg ggtatcgccg gattgtaaac gcaccacata
      481 ttcgcgggct ttatcggcaa aggcgctgtc ctcatcaaag tgttttttgg cttgttggta
      541 aaacacttct aaatctttta gggctaattc cgcctgctcg ccgctgttca tttggttttc
      601 caattcggcc agcaacatgc caaattgcgt gccccaatcg cccacgtggt tttggcggat
      661 taccttatgg cctaaatatt ccagcacccg cactacagca tcgccaataa tggtggagcg
      721 caagtggccc acgtgcattt ctttggcgag gttggggccg gaataatcca ccactatggt
      781 ttgggtttta gccacctttt gcgcctggct cgggttaaac tctgccaccg cctgggccag
      841 ccaagctggg ttgaggtgaa tattaataaa acctgggcca gctacttccg ccttggcgat
      901 cataccgccg gtattaagcg ctgcgccgat ggtttgtgcc aactcccgtg ggttttgccc
      961 catgtgtttg gctgcggcca tggcgccatt ggcttggtaa tcgccgaaac tggggttttt
     1021 actgggggcg atcaccgggt cgcaagtggc gggtacaccc gcttggttca tagcgtggcg
     1081 gacgtgttcg gcgagttgtt ggcgcaggct catatgggtc ggtatgtaat cgcaaataaa
     1141 gggtgcggga ttgtaacctt caagcgcgca tatgcaagct ggccgaatcc tgcgcccgga
     1201 atattcgcta agctttccaa tagtctttta aggaatttgt tatgactgac atttacgtgc
     1261 gctcggccat tgaggcgggc gtgttctaca tctcttgggg taaccctgcc agcaaaaatg
     1321 cgctgtccat tgccatgtat gagcaaatag aggcggccct taaacaggcc gaggcccaag
     1381 aggatataaa agtctgtgta ttgttgggcg gggaagggga tttttgcagc ggcaatgacc
     1441 tggcggaatt tattgccggg cgcaagcagg gcggggcatt ttttaaaccc ttattaaact
     1501 gtattcaccg cttggcgagt tttaaaaaac cgctgttggc ggcggtgaat ggctgggcgg
     1561 tgggcatagg tgccactatg ctgctgcatt gtgatgctgt aattgccgca ccgagcgccc
     1621 aatttagtct gccgtttaat cgcctcggcc tgtgttgtga atgcggtgcg agcctcctgt
     1681 tacctctgcg tatcggccag ggccgggcgg cggattggct gttgcgggct gagcccattg
     1741 ttgctgcgga tgctttgcag gccgggttta ttaaccgtgt gtgtgatgat ccagctgctg
     1801 taatcagtga ttatgcccgc gagatttctg ggcggccctt aagtgcgctg ctcgcccatc
     1861 gcgccctgct gcaagagccc tggcaggaag cacttgccaa ggccatggcg gcggaggaag
     1921 cacgtttttt tcagctgctg caagggccgg aatttgccgc cattgtggcg gccatgagcg
     1981 ccaagtggcg cagcccgaat ccaacagcat cccagcctgc gccctaggag gcacccatga
     2041 ttcaatttga tttcaccggc cagcgggttt tggtagtggg cggcaccagc ggtatcaatc
     2101 gcggtattgc ccgcgccttt gcccagcatg gcgcccatgt ggcggtggtc agccgcagtg
     2161 aggaaaaggt gcaggacacc ctggccgagc tttcccaatt tggcaatgaa gtggcgggct
     2221 ttaccgccga tgtgcgcgaa tatgcgcccc tgtcggaggg catcaaagaa ttggctgaag
     2281 cctgggggcc tttgcatgtg gtggtgtctg gcgcggcggg taatttccct gccctggggg
     2341 cacagatgtc ccccaatgcc tttgcctcgg tggtgaatat cgatctgctg ggtacctacc
     2401 atgtgatgcg cgcggtgtac ccctatttac agcggccggg cgcaagtgtg ctgaatatct
     2461 ccgcgccgca agcgcagttg cccatgctgg cccaagccca tgtgtgcgcc gccaaggccg
     2521 gggtggagat gctcacccgc agcctcgctt gcgagtgggg tggcgagggt ttgcgagtga
     2581 atgccatcat ccccggccct attgccaata ctgagggcat ggctcgctta gcgcccacgc
     2641 cagaattgat ggccgcctgc gcccaatcgg tacccatggg gcgcttaggg gaggtggagg
     2701 atattgccaa tgccgccctg ctgttggcct cgcccctggc gaactttatt actggcgcca
     2761 tagtgccggt ggacggaggc tggctgcagg gcggcgctgc cgcggtggcg gcgagcttcg
     2821 ccagtttgtt aaaaaagtaa gcccctgaaa aacttgacct aagcctgcgg cagcgcaggt
     2881 atttttgcgt acactgtgcc cttttgtttg gaatacct
//
LOCUS       DAOQGS010000538         2915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20676_length_2915_cov_3.901049, whole genome shotgun sequence.
ACCESSION   DAOQGS010000538 DAOQGS010000000
VERSION     DAOQGS010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20676_length_2915_cov_3.901049"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PKZ52_15880"
     CDS             <1..1127
                     /locus_tag="PKZ52_15880"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNG61302.1"
                     /translation="DAKAILLDESDQRMEVRVLAGSLLGNRFKRMWIEGVAINAGTGE
                     MAKHSQNLADFSNLMSIKLQRDDILSIARQGNATVVRVNNLELGKIPSGEFYNLLLRT
                     WIGPVPLSSSFRDGLLTSGKVDEKTISIYSKISPTPERIAVIRAALIAAGKNKGKPTE
                     VAAAKPEPAKVEPVAKVEPPVKEVAKTEPPVKEAVKEVAKVEAVKPVEKAPVKPEAQE
                     PSSPAATATAAAPVAAAAQAASQVAKAATPAASAPKPAAAKANEVFEEEEKQETADSL
                     LVQQLYIAKLSKRTAGFVKYPKSALSKEQEGTVRLAITINRSGQVTNVKSSSTNEVPV
                     LTQAAVEAANRASPYPAIPEQIKSDSFTFTIPITFRVRDR"
     gene            complement(1224..1799)
                     /locus_tag="PKZ52_15885"
     CDS             complement(1224..1799)
                     /locus_tag="PKZ52_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61303.1"
                     /translation="MHGFQEIDFSRRTQNKDLIWDLDTFAQRRTAHEFVMHFENKICV
                     FSPSVEQIYSNYSITFPAEENKRLIILPNPSAHHDIFQSIPEGAVKPTGLYIVPSKQS
                     ELNLRIPLKTQDSPWRDVPLHVGLKLINSRRPRHLPLLPILVKGDLRELDSRTPVLHL
                     HAVSLSALSKLSALELNAIRKVILDRISALQ"
     gene            2005..>2915
                     /gene="radA"
                     /locus_tag="PKZ52_15890"
     CDS             2005..>2915
                     /gene="radA"
                     /locus_tag="PKZ52_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNG61304.1"
                     /translation="MAKTKTAFVCNNCGAEFNKWQGQCSACGAWNSVQEIRLGTQPAS
                     GRAVGYAGSASQVQVLKDINLVDLPRFSSGMQEFDRVLGSGFVPGSVVLIGGNPGAGK
                     STLLLQTLCHLAQEAPALYVTGEESLQQVAMRAQRLSLPNDRLQVAAETDVEAVLYLA
                     GQHKPRIMVVDSVQVMHLSDITSAPGSVSQVRESAAVLTRFAKQSGTCVILVGHVTKD
                     GSLAGPKVLEHMIDCSIMLEGDNDSRFRTLRGNKNRFGAINELGVFAMTEQGMREVSN
                     PSAIFLQRAEEVAAGSVVMVIWEGTRPL"
BASE COUNT          725 a          727 c          807 g          656 t
ORIGIN      
        1 gtgatgccaa agctatattg ctggacgaaa gtgaccagcg tatggaggtg cgtgtgctgg
       61 caggcagcct gttaggcaac cgctttaaac gcatgtggat tgaaggggtg gcgataaatg
      121 ccgggacggg ggaaatggcg aaacactcgc aaaacctcgc cgattttagc aatctcatgt
      181 cgattaaatt acagcgcgat gacattctca gtattgcccg ccagggcaac gccacggtgg
      241 tgcgcgtgaa caacttagaa ctcggcaaaa ttcccagcgg tgaattttat aacctgttac
      301 tgcgtacttg gattggccct gtgcccttat cctccagttt tcgcgatggt ttattaacca
      361 gcggcaaggt ggatgaaaaa actatcagta tttacagcaa aatcagtcca acgcctgagc
      421 gcatcgcggt tattcgtgcc gcattgattg cggccggcaa aaacaaaggc aaaccaactg
      481 aggtagcagc ggctaaacct gagccggcca aagttgaacc tgtagccaag gttgaaccgc
      541 cagtaaaaga agtggcgaaa acagagcccc ctgttaaaga agcggtgaaa gaagtggcga
      601 aggttgaagc ggttaagccg gtggaaaaag cgcccgttaa accagaagcc caggaaccgt
      661 ctagccctgc ggctaccgcc acagctgctg ctcccgttgc ggcagcggcg caagcggcca
      721 gccaagtggc caaagcggct acaccggcgg caagtgcgcc taagcctgca gcagctaaag
      781 ccaatgaggt ttttgaagag gaagaaaagc aggaaacggc tgacagcttg ttggtgcaac
      841 agctctatat tgccaaactc agcaagcgca cggcgggttt tgttaagtac cccaaatctg
      901 ccctttctaa agagcaagag ggtaccgtgc gcctcgccat taccattaac cgctcaggcc
      961 aggttaccaa cgtaaaaagc tccagcacca atgaggttcc tgtactgaca caagctgccg
     1021 tggaggccgc caaccgtgcc tcaccctacc ctgcaattcc cgagcaaatt aaaagcgatt
     1081 cgttcacctt cactataccg attacctttc gagtacgaga tcgttaagtt atttttgttg
     1141 gtttccacct gcaaacagac ccatttaata atgggcctgt ttggttctgc ttaacgcagc
     1201 ctgtggtttt tcagcgcggg tttttactgc agagcactga tgcgatctaa aatcaccttg
     1261 cggattgcat ttaattctag ggcggagagc ttacttaaag cgctcagaga caccgcgtgc
     1321 aggtgcaata ccggagtgcg cgaatctaat tcccgtaaat cgcccttaac caaaataggc
     1381 aatagcggta gatggcgcgg cctgcggctg ttaatcaatt taagacccac atgcaaaggc
     1441 acatctcgcc agggcgagtc ttgggttttc aggggaatgc gcaggtttaa ctcactttgt
     1501 ttgctgggca ctatataaag cccggtgggt tttaccgcgc cttctggaat tgactgaaaa
     1561 atatcgtgat gggcgctagg gtttggcagg ataattaagc gcttattttc ttctgccgga
     1621 aatgtaatgc tgtaattgct gtatatctgc tcaacactcg gcgaaaacac gcagatttta
     1681 ttttcaaaat gcatgacaaa ttcatgggct gttcgccgct gggcaaacgt atctaaatcc
     1741 cagattaggt ctttattttg ggtgcgccgc gaaaaatcaa tttcttgaaa gccgtgcata
     1801 taacctcaat actgtgacaa acctacgggt acgccactta attacccgct cctccaaggg
     1861 caaggaagga atgtttgggg ggataagttg aagcagggaa atccaaccat ttcacattta
     1921 agtgtaagag gccttcgcca gttcgcaagg cctttgatca aaaaaccatc gcaatgccaa
     1981 tctcacctta tttaaggagc cgctgtggcc aaaactaaaa ctgccttcgt gtgtaataac
     2041 tgcggtgcgg aatttaataa gtggcagggg cagtgcagtg cctgtggggc ctggaacagc
     2101 gtgcaggaaa tccgtctcgg cacgcagcca gcgagtgggc gagcggtggg ttatgccggt
     2161 agtgcgagcc aagtacaggt attaaaagat attaacctgg tggatttacc gcgctttagc
     2221 tccggcatgc aggagtttga ccgggtgctc ggcagcggtt ttgtgcccgg ttctgtagtg
     2281 ttaattggcg gcaaccccgg tgcgggcaag tccacgctgc tgttgcagac cctctgtcat
     2341 ctggcccaag aggcccctgc cctttatgtc accggcgaag aatccctgca gcaggtggcc
     2401 atgcgagctc agcgactgag cctccccaat gatcgcttgc aagtggcggc cgaaacggat
     2461 gtggaggcag tgttgtactt agctggccaa cacaaaccgc gcattatggt ggtggactcg
     2521 gtgcaggtga tgcacctgag cgacatcacc agtgcccccg gcagcgtctc gcaggtgcgc
     2581 gaaagtgccg ctgtgctgac gcgctttgcc aaacaatcgg gcacctgcgt aattttggtg
     2641 ggccacgtga ctaaagacgg cagccttgca ggccctaagg tgctggagca tatgatcgac
     2701 tgctcgatca tgctcgaggg tgacaacgac agccggtttc gcaccctcag aggcaataaa
     2761 aaccgcttcg gcgctattaa cgagctgggg gtctttgcca tgaccgagca gggaatgcgg
     2821 gaggtgagta acccttcggc gatttttttg cagcgggctg aagaagtggc ggcaggttcc
     2881 gtggtgatgg tgatctggga aggcacccgg cccct
//
LOCUS       DAOQGS010000539         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20766_length_2908_cov_4.242902, whole genome shotgun sequence.
ACCESSION   DAOQGS010000539 DAOQGS010000000
VERSION     DAOQGS010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20766_length_2908_cov_4.242902"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..648
                     /locus_tag="PKZ52_15895"
     CDS             373..648
                     /locus_tag="PKZ52_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61305.1"
                     /translation="MTRIISNTAKSERRQALSNAIPRNIQNYLSQPQLQTLRQMELLG
                     WRLQFVRREANSPPLVVLRHQDSGKFSVLEDTGRANMKPELRLRSVK"
     gene            909..1214
                     /locus_tag="PKZ52_15900"
     CDS             909..1214
                     /locus_tag="PKZ52_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61306.1"
                     /translation="MNPSDLQTVEEIGAIPLPESFMPRGVMPPVPPAVNEWLDQSQQL
                     SLRTMEYFGWQLWFIRRQQLPAIVVIRDGYSQQFAVLEPGGQINQKPHIQLRADMRN"
     gene            1305..>2908
                     /locus_tag="PKZ52_15905"
     CDS             1305..>2908
                     /locus_tag="PKZ52_15905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG61307.1"
                     /translation="MTVLLPVSPLSLAFYVSAGCAIALGIIGTCVAIYIKRPRARLLF
                     IVLSLLCFTLTAYQLTCAAQFNAADLPAARAASIRQLVLAAVLIPLLLSAQAALLRRR
                     RKPVASVIAAGIGLVYLGVLGIGPDYLGFDSGHLAAVNLVWPLVDNGRAFVPANTPLL
                     FSLAVLVGIAISWALVQAHAAWQQRRLRLCVGFGLFWLLEAVLLLLCWYIQPVIVWRD
                     LAGFPLVLLMIWITAHLAVESVHQGELLQEGKADLKLQVRERQIAENNVRRMAFEDYL
                     TRLPNRMVVHEHIADLLMHRHDNDFRAAFYVLDLDHFKTINDALGHDFGDEVLRCVGQ
                     RLSQHIHTAALIARIGGDEFAVLEERIEGEPRKQIQRTATRILEIMSEPLHVGDHVLD
                     VSCSVGVVTFPTEADTVIDVVRSADIALYEAKKRQRGSYFVFTPQMRELANERLSLEK
                     GLRHALQKDQLSLFFQPQVDADGQQVGAEALIRWNNPAQGFISPLKFIRVAEETGLIN
                     PIGDWVMQKSLLALADWLQKGVPFSG"
BASE COUNT          631 a          672 c          788 g          817 t
ORIGIN      
        1 ttgacaaaaa agcgtgcaga ttcaagcatt aggcggccgt agtagagttc aactgtggtt
       61 tttaggttga ttttgtgtat ctcccttgcc cgtcaaaaaa tgcatataca caaggcgcta
      121 cagtttcctc ttggtctttc acaaccttgc tcacagaggt ctataaatat tcggctcagg
      181 gtacgggctg ctaggcatat gggataattc ccccatgggt ggaacaatat tcgtgatgct
      241 aatctcatcc gtttgccgct atttaatctg tagcacagta tgaacatatg ctgtttgaca
      301 aacatgagaa ttgcgtcgac catcgcagca aaataacaat aacgaactaa aacaataaag
      361 aaggggccat ccatgacccg cattatttcc aataccgcta aatctgagcg tcgccaggcg
      421 ctctctaatg ctattccgcg caatatccaa aattacctca gccagcccca gctgcaaact
      481 ctgcggcaaa tggagctatt aggctggcgt ttgcagtttg tgcggcgcga agctaatagc
      541 ccacctttgg tggttttgcg ccatcaggat tcgggcaaat tttctgtctt ggaagataca
      601 ggccgcgcca atatgaaacc ggaattgcgc ctgcgctcag taaaataggc tgtgccactg
      661 gttccaacga ttttgtctcg tccgcacccg gcagctgagc ctggccggta attggcgctg
      721 ggaaactcgc gggtgtttta tagtgaacgg ccattccttt gcggttttta ggtttgattt
      781 tttcttatta acacgttttg ttattgcaat tgcatctatc atgctgcgtg gcagcagaaa
      841 attttgacaa atgcctcgat aaggtcaaag ctctataggt cttacaccta aaacagggct
      901 ttttggccat gaacccttcc gacctgcaga ctgtagagga aataggcgcc atccctctgc
      961 ctgagagctt tatgccgcgc ggtgtgatgc cgcctgttcc gcctgctgtg aatgaatggc
     1021 tggatcagtc gcagcaatta tccctgcgca ccatggaata ttttggttgg caattatggt
     1081 ttattcgccg tcaacaatta ccggcaatag tggttattcg cgatggctac agccaacaat
     1141 ttgcggtttt agagccgggc ggacaaatta atcaaaaacc gcacattcag ctgcgtgcgg
     1201 acatgcgtaa ttaaacccgg tggccagagt gtaaaaaaca gcgtttgcgc cgctgttatc
     1261 tgatgcctta tttcatggcc tgctaattcc tgttgttgag ttctatgact gtgttgttgc
     1321 ctgtttcgcc gttatccttg gcgttttatg tatccgcagg ctgcgcaatt gcattgggaa
     1381 ttatcggcac ctgtgtggcg atttatatca aacgcccccg cgcacgcctg ctgtttattg
     1441 ttttatccct cctgtgtttt accctcactg cttatcaact aacctgcgcc gcacaattta
     1501 atgccgccga tttgccggct gcccgagccg cgagcattag gcagctggtg ttggccgcgg
     1561 tattaattcc tttattgtta agtgcccagg cggcgctttt gcgcaggcgg cgcaaacctg
     1621 tggcgagtgt tatagccgca ggcataggcc tggtgtattt aggggtgttg ggtatagggc
     1681 ctgattattt gggttttgat tccggccact tagccgcagt gaatttagtt tggccgctgg
     1741 ttgataacgg gcgcgcattt gtgcctgcga atacgccgct tttattctct ctggcggtgt
     1801 tggttggcat tgccatcagt tgggcgctgg tgcaggcgca cgcggcttgg cagcagcgca
     1861 ggcttaggtt gtgtgttggt tttgggctgt tttggttgtt ggaagcggtg ctgttgttgc
     1921 tctgttggta tatacaaccg gtaattgttt ggcgggattt agcgggtttt ccgctggtgt
     1981 tattgatgat ttggattacc gcccatttgg cagtggaatc tgtgcatcag ggggaattat
     2041 tgcaagaggg caaggcggat ttaaaactgc aggtgcggga gcggcagatt gcggaaaata
     2101 atgtgcgccg tatggcgttt gaagattacc tcacgcgctt gcccaaccgt atggtagtgc
     2161 atgaacacat cgccgattta ttgatgcatc gccacgataa tgattttcgc gcggcgtttt
     2221 atgtcttgga tctggatcat ttcaaaacca ttaacgatgc cctcggccat gattttggcg
     2281 atgaggtgtt gcgctgcgtg ggccagcgct tatcccagca tattcacacc gccgctttaa
     2341 ttgcgcgcat cggcggcgat gaatttgcgg tgcttgaaga gcgcattgag ggggagccgc
     2401 gcaaacaaat tcagcgcact gccacgcgga ttttggaaat catgagcgag cctttgcatg
     2461 tgggcgacca tgtattggat gtgagctgct cggtgggagt ggtgacgttt cccaccgagg
     2521 ccgatacggt aattgatgtg gtgcgctcgg cggatattgc tttatatgaa gcaaaaaaac
     2581 gccagcgggg cagttatttc gtttttaccc cgcaaatgcg cgagttggcg aatgagcgtt
     2641 tatctttaga aaaaggcctg cgccacgccc tgcaaaaaga tcagttatca ctgtttttcc
     2701 agccccaggt ggatgcagac ggtcagcagg tgggggccga ggcgttaatt cgctggaata
     2761 atccggccca gggttttatc tcgccattaa aatttattcg cgtcgcggaa gagactggtt
     2821 taattaatcc tatcggcgat tgggtgatgc aaaaatccct gctggccctg gccgattggc
     2881 tgcaaaaagg cgtgcccttt agcggcag
//
LOCUS       DAOQGS010000540         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20812_length_2904_cov_5.268515, whole genome shotgun sequence.
ACCESSION   DAOQGS010000540 DAOQGS010000000
VERSION     DAOQGS010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20812_length_2904_cov_5.268515"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKZ52_15910"
     CDS             complement(<1..619)
                     /locus_tag="PKZ52_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNG61308.1"
                     /translation="MTLFDWAGSAAAWGLWGVLALPLLLLGAAHRLGAWRLAQWLGGA
                     GLLLALALWCAALPSPLTSGVLRLGFVQASLLVLVSLLGWLIYRYGASNFRGDQDGPR
                     FLRGLAGVLLAVQTLLLADHLLLFWAAWVMVSISLHQLLLFYPERPRARLAAHKKFLI
                     ARSAEALLALAIGLLYSQSGSFSLAANMAQLTGGELAWPLTLAALC"
     gene            734..1615
                     /locus_tag="PKZ52_15915"
     CDS             734..1615
                     /locus_tag="PKZ52_15915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG61309.1"
                     /translation="MSKLNYHHLYYFWRVAKQGNLTKTAASLHISQSALSAQIRQLEA
                     SLGVELFSRASRALHLTDSGQSALAYAEEIFTRGEELESLLSQGIQPAVRRVRIGVLT
                     TMSRNFVESFIEPLIRRPNTQYLLQARGQVNLLNALASHEFDLALTNIQVQGGQAELW
                     QCQLLARQPIAILGPPQLPLEKVFSKRYEACEWVAPVADSPIRSAFDSFCAQQQFKPR
                     IVAEADDMAMLRLLARDTGALAVMPEVVVKDELSQGKLVNYLTLPNAFENFYAVTVKR
                     QLPNPLVSELISRGIKL"
     gene            complement(1842..2630)
                     /locus_tag="PKZ52_15920"
     CDS             complement(1842..2630)
                     /locus_tag="PKZ52_15920"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HNG61310.1"
                     /translation="MTHRVKLSAFWVANQFDLKGIKAFLDIKPLADSSSELFYGFAGE
                     KFQYYFNFGVLVLSGYTEDEMKWAIKTVHNFQRDPSENWMRDDHAINVAADSDIVFGF
                     DELTVAKVDAQVIRISMFNLAQSVALDHYHEVSENLLTEVKGFTDQLEHTGKLKISRS
                     NMRRFLGRALNTQIDIAENIYIFDAPDLVWYDDYLDKLHQGLMKHFDLRVRFSEVEYT
                     MRIIAENLAAFREIIHQRESNFLEYIIIALIFVEVLDLIIAKLL"
BASE COUNT          740 a          830 c          734 g          600 t
ORIGIN      
        1 aacacagggc ggccagagtt aggggccagg ccagctcccc gccggtaagc tgggccatat
       61 tggcggcgag ggaaaaactg ccggactggc tgtacaacaa gccgatggca agggccaaga
      121 gggcctcggc gctgcgggcg atgaggaatt ttttatgggc cgcaagcctg gcgcgggggc
      181 gctcggggta gaacaacaac agctggtgca ggctgatgct caccattacc caggcggccc
      241 agaacagcaa cagatggtcg gccagcagca gggtttgcac cgccaacagc acaccggcaa
      301 ggccgcgtaa aaagcgcggc ccgtcttgat cgccgcgaaa attgctagcc ccatagcgat
      361 agatcagcca gccgagcaaa ctcaccaaca ccagcaggct cgcctgcaca aaccccaagc
      421 gcagcacccc gctagttaag ggcgagggca aggcggcaca ccacagcgcc agcgccaaca
      481 gcagccccgc gccccccagc cactgggcca gccgccaagc gccaagccta tgggcggccc
      541 ccagtaacaa aagcggcagc gccaataccc cccaaagccc ccaggctgcg gcagagcctg
      601 cccaatcaaa tagcgtcatg tttacctccc cggataaaag cgaatgcggg gggaattctg
      661 ggctagccta aaccataggt aaaatacata atttgatcga tttcattcac aaaaacagaa
      721 cgaaaccccg gccatgagca aactgaacta ccaccacctg tattactttt ggcgcgtggc
      781 caagcagggc aacctcacca aaaccgccgc cagcctgcac atttctcagt ccgccctatc
      841 cgctcaaatc cgccagttag aggccagcct gggggttgaa ctcttcagcc gcgcaagccg
      901 cgctctgcac ctcaccgaca gcggccagag cgccctggcc tacgccgaag agatttttac
      961 ccgcggcgag gagcttgaat ccctgttgag ccagggcatt caaccggctg tgcggcgggt
     1021 gcgcatcggt gtgctcacca ccatgtcgcg caactttgtg gagagtttta ttgaaccgct
     1081 tatccgccgg cccaacaccc agtacctgtt gcaggcgcgg ggccaggtga atctgctaaa
     1141 cgccctggcc agccacgaat tcgacctggc ccttaccaac attcaggtgc agggcggcca
     1201 agccgaactc tggcaatgcc aactgctcgc ccgccaaccc atcgccatcc tcggcccgcc
     1261 ccagctgccc ctggagaagg tgttttccaa acgctatgag gcgtgcgaat gggtagcccc
     1321 agtggccgac agccccatcc gctcggcctt cgacagcttc tgcgcccaac agcaatttaa
     1381 accccgcata gtcgccgaag ccgacgacat ggccatgctg cgcctgctcg cccgcgacac
     1441 aggggcgctg gcggtcatgc cggaggtagt ggtaaaggat gaattatccc agggcaaact
     1501 ggttaattac ctcaccctgc ccaatgcgtt tgaaaatttt tatgcggtga cagtaaaacg
     1561 gcagctgccc aatccgttgg tgagtgaatt aattagccgc gggattaaac tctaagagaa
     1621 tctgattagg atttgagaat tttttaaaac tcagaaatac gttgctatta acccggtggc
     1681 aaaatagatt gttctgatct attttgcctt cagctccgct gagccttgcg aaaatgcgtg
     1741 gattttcttg ttttagcgga gcttacggcg gcttgcgcca cctgtgcggt atccttgccg
     1801 cacttattaa cccggcataa cagccgtcac ggatttatct atcacaaaag tttggcgata
     1861 attaaatcca atacttcaac aaaaattaac gcaataataa tgtactcaag aaaattgctc
     1921 tcccgctggt gaatgatctc ccgaaaggct gccaggtttt cggcgataat gcgcatggtg
     1981 tactccacct cgctaaagcg cacgcgcaaa tcaaaatgtt tcatcaaacc ttgatgcagc
     2041 ttatctaagt aatcgtcgta ccaaactaga tctggggcat caaagatata aatattctca
     2101 gcaatatcaa tttgtgtatt gagggcgcgg cctaaaaagc gacgcatatt ggatcgacta
     2161 attttaagtt tgccggtgtg ctccaattgg tcggtgaagc cctttacttc agtgagcaga
     2221 ttttccgaca cttcgtggta atgatctaaa gccaccgatt gggcaaggtt aaacatggag
     2281 atgcgaatca cctgggcatc caccttggcc actgtcaatt catcaaagcc aaacacaata
     2341 tcgctatcgg cagcgacgtt tatagcgtgg tcatcgcgca tccaattctc gctggggtcc
     2401 cgctggaagt tatgtacggt tttaatggcc catttcatct catcctcggt atagccagac
     2461 agcaccagaa caccaaaatt aaaataatac tggaacttct cccctgcgaa gccgtaaaac
     2521 agttcagagg aactatcggc gagcggttta atatcgagaa atgccttgat gccttttaaa
     2581 tcaaattgat tggcaaccca aaacgctgag agttttaccc tgtgtgtcat aaagttgcct
     2641 tggcgagtga gaaataattg ctcaagaagt gtatttttaa agtactgccg agcgcggcct
     2701 cattctagcg aatgcccagc cgcgcccggt tacattttta tgacgttttc actcgcccgg
     2761 ctagttgctt attggataca taggcggcga cgcccagcca aaatagaaca ccgcccgcgc
     2821 tggcaaggaa gtagttcagc tattaacccg gcggcaatta gtgccgggtt aataaaggcg
     2881 gcaaggatgc cgcatcaaag gtgc
//
LOCUS       DAOQGS010000541         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20813_length_2904_cov_4.661636, whole genome shotgun sequence.
ACCESSION   DAOQGS010000541 DAOQGS010000000
VERSION     DAOQGS010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20813_length_2904_cov_4.661636"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..2149)
                     /locus_tag="PKZ52_15925"
     CDS             complement(53..2149)
                     /locus_tag="PKZ52_15925"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNG61311.1"
                     /translation="MRSVIQLLFLCAPTRRFVANRYRLASLGILAVILTLTGSPISRA
                     AEIPTASASAQRINSTLAAHNINQNEEEDNAAAQVVASPFLNIPAVNLSRVKPTTPLE
                     PYVFYLPDTNGGLDALQAMRALPNFMPLQKKPITFQTKSHWYALPLHNDLNMPVERFI
                     TTGVSTAPLLRAYWISQEIAANPQDIKPIASQLAYGFPTLLFNLPINSGESGVFLIEY
                     QSIAHFPITLHLYDAAQLFEATQRLKMANGIYIGAIGILFLFFFGQFLISREKVYFYY
                     SMFVLFAILIMMQISGPAQLFNQDSHHQTMLTTLIGGGIYTFYFIFTAEFLQFKQRNK
                     FLYRALLILSASVLALTAISIVYPSAYALSVVIAIGLPFPIAGAIWAWRQKISSALFF
                     LVGSLTHCISTYFLLFACLGFAPFRNELIFRIACLGMMFDIICFALAIIYHSHQVRIH
                     YNRQLQERIDDLNALAESERLSAQALSMSKQAVLNLAATAHDLQQPLSAMQLLLSLQN
                     SADPAIQNVQGALNYACSLLNSALGTARQDYQTFAEMVDSKSLLGAAQARHQARFSEK
                     KITLKMRSPAHRLKCLPIVVNRILDNLLSNAHKYTHSGTVLIAGRKRKNGDFLIQIWD
                     TGRGMDHKQTQRLTTPFERIQDTEELGFGLGLFIVKSLCHQAGYHFAIHSKEQRGSCV
                     SILIPHSQSKQDWLEV"
     gene            2177..2785
                     /locus_tag="PKZ52_15930"
     CDS             2177..2785
                     /locus_tag="PKZ52_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG61312.1"
                     /translation="MNILIADDHDIFRQSLALLIEARSSHRVIAHAQSFSGLVEQAIR
                     VAPDCMLLDYQMPGGDPLSLSEQLRSQMPELKLILLTGIQSGAALQQLEATLCDGLLH
                     KRDNADTILQGIAEVAEGRHFVSATVAELIADAQVGLTQRELQVLHLFAQGISLHAIA
                     ENLSISLRTVEKHKENIMKKLNVVNSIQLVEAAHKLWVGGVQ"
BASE COUNT          773 a          619 c          801 g          711 t
ORIGIN      
        1 tttgccaaat tcaacgacgc cagggcgagt aaggtaattt ttcagaggtt ccctaaacct
       61 ccagccaatc ctgcttggat tggctgtggg gaattaaaat actcacacaa ctgccgcgct
      121 gttctttgga gtgaatagcg aaatggtagc ccgcttggtg gcagagggat ttgacaataa
      181 ataaccctaa gccaaagcct aattcttcag tatcctggat gcgctcaaaa ggcgttgtta
      241 ggcgctgggt ttgcttgtga tccatgccgc gcccggtatc ccagatctga attaaaaaat
      301 cgccattttt acgtttgcgg ccagcaatta atacggtgcc gctgtgggtg tatttatggg
      361 cgttggacag taaattatcc aaaatgcgat taaccactat gggcaggcat ttaagcctat
      421 gggctgggct gcgcattttt aaggtgattt ttttctcact aaagcgcgct tggtggcgag
      481 cctgggcggc gccaagcagg cttttgctat cgaccatttc agcaaaggtt tgataatcct
      541 ggcgggcggt gcccagagcg gagtttaata acgaacaggc gtaattaagc gcaccctgta
      601 cgttttgaat ggctgggtcg gctgaatttt gcagggataa taataactgc atggccgaga
      661 ggggctgctg taaatcgtgg gcggtggcgg ctaaattcag tactgcctgc ttagacatac
      721 taagcgcctg cgcggagagt cgctcacttt cggccagggc atttaaatca tcgatgcgct
      781 cttgtaattg tcggttgtaa tgaatgcgca cctggtggct gtggtaaata atcgccagag
      841 caaaacaaat aatatcgaac atcatcccaa ggcaggcgat gcggaaaatc aactcattgc
      901 gaaagggggc aaagcctaaa caggcaaata ataaaaaata ggtggaaatg cagtgggtga
      961 gcgaacccac caaaaaaaac agggccgagg agattttttg ccgccaggcc cagatggccc
     1021 ctgcaatagg aaaaggcagg ccgatagcaa ttacgacgct cagcgcatag gcgctggggt
     1081 agacaatact gatagcggtt aaggccaata cgctggcact caggattaac agcgcgcggt
     1141 agaggaattt attgcgctgt ttaaactgca aaaattcagc ggtaaaaata aagtagaaag
     1201 tataaatgcc gccgcctatg agggtggtga gcatggtttg atggtggcta tcttgattga
     1261 acagctgcgc agggccactt atttgcatca tgattaaaat ggcaaataat acaaacatag
     1321 aataataaaa gtagaccttc tcgcggctga taaggaactg gccaaagaaa aacaaaaata
     1381 aaatgccaat ggcgccgatg taaataccat tggccatttt aagccgctgg gtggcctcaa
     1441 acagctgcgc ggcatcataa aggtgtagtg tgatggggaa gtgggcaatg ctttggtact
     1501 caattaaaaa tacgccggat tcaccgctat ttatgggtag attaaatagc aacgtgggaa
     1561 acccataggc cagttggctg gcaatgggtt taatatcctg cggattggcg gcaatctctt
     1621 ggcttatcca ataggcgcgc aaaagcgggg cggtggatac gccggtagta ataaagcgct
     1681 ccacaggcat attaagatca ttgtgcaatg gcagcgcata ccaatgggat ttggtttgaa
     1741 aggtgatggg ctttttttgc aacggcataa agttgggcag tgcgcgcatc gcctgcagtg
     1801 catccaggcc gccgttggta tccggtagat aaaagacata gggttcaagc ggtgtggtgg
     1861 gttttacacg gcttaaattc accgctggga tattgagaaa tgggctggcc accacttgcg
     1921 ctgccgcgtt atcctcttct tcattctgat taatattatg tgcggcgagg gtggaattaa
     1981 tccgctgcgc gcttgcgctt gcggtgggga tctcagctgc gcggcttatt gggctgcctg
     2041 ttagggtgag aataactgct agaatgccaa ggcttgcaag cctgtacctg ttggctacaa
     2101 acctgcgggt gggcgcgcac aaaaataaca attgaataac ggaacgcatg gcgcttttca
     2161 acggggtatc tcttttatga acattttgat cgcggatgat cacgatattt ttcggcaatc
     2221 cctggcgctt ttgattgagg cgcgctccag ccatagggtg atagcccatg ctcagtcctt
     2281 tagcgggcta gttgaacagg ctatccgtgt ggcgccagat tgtatgctac ttgattatca
     2341 gatgccaggc ggagaccccc tgagcctgag cgagcaattg cgcagccaaa tgccggagtt
     2401 gaaattaatt ttattaaccg gcattcaatc gggcgcggcg ctgcaacagt tagaggcaac
     2461 cctgtgcgat gggttattac ataaacgcga taacgccgat actattttgc aggggattgc
     2521 tgaggtggcc gagggcaggc attttgtctc cgcgacggtg gcagaattaa ttgccgatgc
     2581 tcaagtgggg ctgactcagc gggagttgca ggtgttacat ttatttgccc agggcataag
     2641 cttgcatgca attgccgaga acctctccat atccctgcgc acagtggaaa aacacaaaga
     2701 aaatataatg aaaaaactca atgtggttaa ctccatacaa ttggtggagg ccgcgcacaa
     2761 attgtgggtg gggggcgttc aataatggct gggcaggaag ttatattgca ctgtttgatt
     2821 gcttcagcct gctgcaaaat taccgctcgc ttactagctg tacggaagca atgaatacac
     2881 atcatttcag accgtcaaaa agag
//
LOCUS       DAOQGS010000542         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_20815_length_2904_cov_4.140751, whole genome shotgun sequence.
ACCESSION   DAOQGS010000542 DAOQGS010000000
VERSION     DAOQGS010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20815_length_2904_cov_4.140751"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKZ52_15935"
     CDS             <1..456
                     /locus_tag="PKZ52_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNG61313.1"
                     /translation="VGIFMILGYKVFGLTANIALCINLVMLLAIMAVMGATLTLPGIG
                     GIVLTMGIAVDANVLIFARIKEELEAGLQPHAAIVAGFDRAFITILDSHLTTMLVAAI
                     LFAIGTGPIKGFAATLFVGISTSLFSAIVGSRSMVNLIYGRRNVTKLHI"
     gene            503..1405
                     /gene="secF"
                     /locus_tag="PKZ52_15940"
     CDS             503..1405
                     /gene="secF"
                     /locus_tag="PKZ52_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNG61314.1"
                     /translation="MGEPHRRVLMFVSLILTILSLVSLGTKGLKFGLDFTGGTSLELR
                     YTQAPDLKQVRETLVSAGYPSAVVVNFGEPTDILVRVQSSAANNELGVVLTNILRKAS
                     GQEITIKQSEYVGPHVGEELMNSGGIGLLVAILGVFLYVAVRFQFKFAMGSILALLHD
                     VVLILGVFSFFQLDFDLTVLAAVLTIIGLTINESIIVADRVRENLRIMRDADVKHVIN
                     TSITQTLSRTIMTSGSINMSVLAMFLFGGEAFHHFSLALLFGVTFGNYSSVYIASGLL
                     VTLKVTREDLIPAEVVQDEVDEMP"
     gene            complement(1543..1692)
                     /locus_tag="PKZ52_15945"
     CDS             complement(1543..1692)
                     /locus_tag="PKZ52_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61315.1"
                     /translation="MWSKLSCSLKDELEYLFAAMAEAALPDQLWLPTQLASASSTQAP
                     KPAQH"
     gene            1811..>2163
                     /locus_tag="PKZ52_15950"
     CDS             1811..>2163
                     /locus_tag="PKZ52_15950"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNG61316.1"
                     /translation="MFKALLQSLFSRRSAAEEQDIADMRASLQRHQRIFRSSHGYGYF
                     DWDLKKQKMDWSGGYWKYLGYTDEDVEVISMSSNYFDYVHPEDVSRMQEAIWKLFKAH
                     TLMGEVTYRVRKKNGG"
     assembly_gap    2164..2173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2241..>2904
                     /locus_tag="PKZ52_15955"
     CDS             2241..>2904
                     /locus_tag="PKZ52_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNG61317.1"
                     /translation="MTFVSGIAFDVTKQKQMQQALQLSEERHARILQSSNDGIWEWVL
                     DQDGFHYSSRCWEHLGYTENHEAIAQGVDHMAAWRKRMHPDDLQQFDRVLAQHIRKQG
                     PFDVEYRIRGKGDRWFWVRARGQMVFNEKGEPVRMSGSNMDITELKEAEERVIKAKED
                     AEKASQAKSEFLSSMSHELRTPLNAILGYSQILAMDDSLAPVQRAHVAEITRAGDILS
                     RLI"
BASE COUNT          677 a          660 c          747 g          810 t
ORIGIN      
        1 gtgggtatat ttatgatttt gggttacaag gtgtttggtt taaccgccaa tatcgccctg
       61 tgtatcaacc tggtcatgtt gctggcgatt atggctgtta tgggcgccac cctcacattg
      121 cctggtatcg gcggtattgt gttaaccatg ggtatcgccg tggatgccaa cgtattgatc
      181 tttgcccgta taaaagagga gttggaggct gggttgcagc cccatgcggc cattgtggca
      241 ggctttgatc gcgcctttat taccattctc gactcccact taaccaccat gttagtcgcc
      301 gccattttgt ttgccatagg cacaggccct atcaagggct ttgcggctac tttattcgtc
      361 ggtatttcca cttctttgtt tagtgccatt gttggttcca ggtccatggt taatttgatt
      421 tatggccgcc gcaacgtaac caaacttcat atttaattgc tctgggatta caacaatgat
      481 tacaaaaaat gcgattaact ttatgggaga acctcatcgg cgggtgctga tgtttgtttc
      541 cctaatactc accattctct ccttagtaag cttgggcact aagggcttaa agttcggctt
      601 ggattttact ggtggcacct cgctggaatt gcgctacacc caagcgccag acttaaaaca
      661 agtgcgcgaa accctcgtat ccgccggtta tccgtctgca gtggtggtaa attttggcga
      721 gccaacggac attttggtcc gtgtacaaag ttctgcggcc aataatgagt taggtgtggt
      781 actcaccaat attctgcgca aagcctctgg ccaggaaatt accatcaaac aatcggaata
      841 tgttggcccc cacgtgggcg aggagttaat gaactctggc ggtattggcc tgttagtggc
      901 tattttgggc gtatttttat acgttgctgt gcgctttcaa tttaaatttg caatggggtc
      961 gattctcgca ttgctgcacg acgtagtatt aatcctcggc gtgttctcgt tctttcagtt
     1021 ggattttgat ttaaccgtat tggccgcagt gttaaccatc atcggcttga ccatcaacga
     1081 atccattatc gtggccgata gggtgcgtga aaatttgcgc atcatgcgcg atgctgatgt
     1141 taagcatgtt attaacacct ccatcactca aaccctaagc cgtaccatca tgacttccgg
     1201 ctcaatcaac atgagtgtgc tggctatgtt tttgtttggc ggagaggcct ttcaccactt
     1261 ttcattggcg ctgttatttg gcgtaacctt tggtaactac tcctccgttt atatagcctc
     1321 gggtttgttg gttactttga aggtgacccg tgaggatctg attcctgccg aagtggtgca
     1381 ggatgaagtg gatgaaatgc cataaaaaaa gggccgcaag gccctttttt tatgggggat
     1441 acagtatggc tcctctctac cagtcgccca ttaactacac cagccatcta ttacctacgc
     1501 caacaacctt aagtggctgg acgaatatgg ccaaggttag gtttaatgct gcgcaggttt
     1561 aggtgcctgt gtactgctgg cagaggctag ctgcgtcggc agccagagtt gatcgggcag
     1621 ggcggcctcg gccattgcgg caaataaata ttccagttca tctttaagcg aacagctcaa
     1681 tttgctccac atatcatgct cctcacaggc gtgctccatt ggagtatagc ggctttaggt
     1741 tcgtgcttaa ggtcgcttcg tcatagtggt ttagggttcg ggctgttatt ggttctatag
     1801 gaattaacac gtgtttaagg ctctacttca atcgttgttt tcccgccgat cagcggcgga
     1861 ggagcaggat attgcggata tgcgcgcctc cttgcagcgc catcagcgta tttttcgctc
     1921 cagccacggc tatggttatt ttgactggga tttaaaaaaa caaaaaatgg attggagcgg
     1981 cggttattgg aaatatttag gttatacgga tgaagacgtg gaagttattt ccatgtcctc
     2041 caactatttt gattatgtgc atccggaaga tgtcagccgc atgcaggagg ccatctggaa
     2101 attatttaag gcccacactc tgatggggga agttacctac cgggtgcgca aaaaaaacgg
     2161 cggnnnnnnn nnnagctggt cgatgtgcta gaggcctacc tgaaggtcgc gccgacgcca
     2221 cgcgagatga acagggctgg gtgacctttg tcagcggtat cgcatttgat gtcaccaaac
     2281 aaaaacaaat gcagcaggcg ttgcaattaa gtgaagagcg ccatgcgcgt attttgcaat
     2341 cttccaacga cggcatttgg gagtgggttt tagaccagga tggttttcat tattcctccc
     2401 gctgctggga gcatttaggt tatacggaaa accacgaagc catcgcccaa ggggtggatc
     2461 atatggcggc ttggcgcaag cgtatgcacc cggatgacct gcaacaattt gaccgtgtat
     2521 tagcccaaca tattcgcaaa caaggcccct ttgatgtgga ataccgcata cgcggaaaag
     2581 gcgatcgctg gttctgggtg cgcgcccgcg ggcagatggt atttaatgaa aagggtgagc
     2641 cggtgcgtat gtctggctcc aatatggata ttactgaatt aaaagaagcc gaagagcggg
     2701 taataaaagc taaggaagat gccgaaaagg ccagtcaagc caaaagtgaa tttctctcca
     2761 gcatgagcca cgaattgcgc acaccgctca atgctatttt gggttattca caaattttag
     2821 ccatggatga cagcctagca cccgtgcagc gcgcccatgt ggcggaaatt acccgcgccg
     2881 gggatatttt atcgcgctta atta
//
LOCUS       DAOQGS010000543         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21085_length_2882_cov_3.599929, whole genome shotgun sequence.
ACCESSION   DAOQGS010000543 DAOQGS010000000
VERSION     DAOQGS010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21085_length_2882_cov_3.599929"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKZ52_15960"
     CDS             complement(<1..235)
                     /locus_tag="PKZ52_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNG61318.1"
                     /translation="MEPKPNAQEIYERLRSMIMSLQLMPGSRITENQLADTDHQRPGL
                     TSKYFSVPKGIPPHLRSPASGCGHGSRADPGPSR"
     gene            532..1368
                     /locus_tag="PKZ52_15965"
     CDS             532..1368
                     /locus_tag="PKZ52_15965"
                     /inference="COORDINATES: protein motif:HMM:NF015466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HNG61319.1"
                     /translation="MPTQVIQGFNAPAQLHPRSSQSQVKALCAAMALSLGFAAQGVSA
                     GAAKWSSTNFQYLYGDSYAQIGVDENGKLGSTEVSSSVITLEHVNGWKYGDNFFFVDI
                     TNSDRGDPNKPTSYYGEISPRLSFSELTGLDMSAGIIKDVLFTATAEIGQGFHNNLYG
                     LAVDLNIPHTPVAQINYYIRNEIGEGKDLGSQVTLVWLTPFSLGSVDFAFEGFFDYAF
                     GMDHAENNIITAPRLLVDVGNAWGAKGTLQMGIEYQIWRNKYGIKDIDEDVAQAMVKW
                     IF"
     gene            1435..2616
                     /locus_tag="PKZ52_15970"
     CDS             1435..2616
                     /locus_tag="PKZ52_15970"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNG61320.1"
                     /translation="MTNLINEVCEEEPTAAQKNALPPLRRRDFVRLSALGAAAYAGVK
                     APPLFAADKDKVVMGFIYVGPRDDYGYNQAHFEGKTALAKLDWVKAVDEERVPETIEV
                     QKTMDSMINLSGAQVLFPTSFGYFDPHILKYAKKYPKVMFLHCGGLWDEAKHPKNVGS
                     YFGYIDEAVYISGLVAGHMSKTGKLGFIAAMPIPQVLRNINSFTLAARSVNPKITTSV
                     VFTGGWSNPVKEAEAANSLIDQGVDVLTCHVDSPKVVVETAEKRGIFSCGYHTNNAAL
                     APKGFLTGAEWNWSQLYLDYAALLKEGKPIPNLMRGGIKEGIVKVSPYGKVVSDAAKA
                     AADAKIKQFMQGDFVVYQGPIKDNTGKEVIPAGKSYGQKDIALESMDWLVEGVVGSAK
                     S"
BASE COUNT          770 a          658 c          737 g          717 t
ORIGIN      
        1 cccgcgacgg acccgggtcc gctcggctcc catgaccaca acccgatgca ggagaacgaa
       61 gatgcggtgg aatccccttt ggaaccgaaa agtatttcga agtcagcccc ggcctctgat
      121 gatcggtatc cgccagctga ttctctgtaa tacggctgcc cggcattaat tgtaacgaca
      181 taatcatgct gcgcaggcgt tcataaattt cttgcgcgtt gggttttggc tccatggcgt
      241 ttttttcagt agaacaggga attagcagtg ttatgcatag ccccatataa agcaagccga
      301 aacctgcgcg caaaaatggg gcgcaaggcc tcatactggg ctaaaaatgg gctctttagg
      361 gggagtcggg ataggcgtgg gtagccgccc ccacctgcag ccaagcaatt gatttatagc
      421 tatttttacc cgctaaatgg ccctctggcg cggcgcctgc atcttgtata caagttagta
      481 tccatcaaga tatacaagaa actatatacc ggacatcaag aggttagcgc catgccaaca
      541 caggtaattc aaggctttaa tgccccagca caattacacc cccgctcaag tcagtcacag
      601 gtcaaagccc tttgcgccgc catggcgttg agtttggggt ttgcggccca gggcgtaagc
      661 gccggggcgg ctaaatggag ttccaccaat ttccaatatt tatacggcga tagttacgcg
      721 caaattggtg tggatgaaaa cggcaaactc ggttcaaccg aggtgagtag ctcggtaatt
      781 accctggaac atgtcaatgg ttggaagtac ggcgataatt ttttctttgt ggatatcacc
      841 aatagcgatc gcggcgaccc gaacaaaccc acctcttatt atggtgaaat ctccccccgc
      901 ttaagttttt ctgagttaac cgggctggat atgtcggcgg gtattattaa agatgtatta
      961 tttactgcca cggccgaaat tggccagggt ttccacaata atttatacgg cttagcagtt
     1021 gatttaaata ttccccacac gccagtagcg cagattaatt attacatacg caatgaaatt
     1081 ggcgaaggta aggatttggg cagccaagta accctggtgt ggttaacgcc gttttcccta
     1141 ggcagtgtgg attttgcctt tgaaggattt ttcgattacg ccttcggtat ggatcatgct
     1201 gagaataaca tcatcaccgc cccaaggctg ctggtggatg tgggtaatgc ctggggcgcc
     1261 aaaggcacgc tgcaaatggg cattgaatat caaatatggc gcaataaata cggcataaaa
     1321 gatattgatg aagatgtggc gcaagccatg gttaagtgga tattttagtt gatgcctcaa
     1381 ggcagtaaac cgataaaaac tcaatagtaa acagcctgga tagaggatgg ccctatgact
     1441 aatttaataa atgaggtgtg tgaagaagaa ccgactgccg cacagaaaaa cgctctaccg
     1501 ccgttgcgca ggcgggattt tgtacgtctc tcggcgctgg gggctgccgc ctatgcaggc
     1561 gtaaaagcgc cgcccttgtt cgcagcggat aaagataagg tggtaatggg ttttatttat
     1621 gtcggccccc gcgacgatta cggctacaac caagcccatt ttgaaggcaa aaccgcactg
     1681 gcaaaactcg attgggtaaa agcagtagat gaagagcgcg tgcccgagac cattgaagtg
     1741 caaaaaacca tggacagcat gattaattta agtggtgccc aggttttatt cccaacctcc
     1801 ttcggctatt ttgacccgca tattttaaaa tacgccaaaa aatatcccaa ggtaatgttt
     1861 ttacactgcg gcggcctgtg ggatgaagct aaacacccta aaaatgtcgg cagttatttt
     1921 ggatatatcg atgaggcggt gtacatcagc ggtttagtgg caggccatat gagtaaaacc
     1981 ggtaaattag gttttattgc cgctatgcct attcctcaag tgctgcgcaa tatcaatagt
     2041 tttaccctgg cggcccgcag cgttaacccg aaaatcacta cctctgtagt atttaccggc
     2101 ggttggtcta acccggtaaa agaagctgaa gcggctaata gtttgattga tcagggcgtg
     2161 gatgttttaa cttgccatgt ggatagccct aaagtagtgg tggaaaccgc cgaaaaacgc
     2221 ggcatttttt cctgcggcta tcacaccaac aatgcggccc ttgcccccaa aggtttttta
     2281 accggcgcgg agtggaattg gagccagctc tatttggatt acgcggcact attaaaagag
     2341 ggtaaaccga ttccgaattt aatgcgcggc ggtatcaaag aaggcattgt aaaagtttct
     2401 ccctatggca aggtagttag tgatgctgcc aaagcggcgg cggatgccaa aattaaacaa
     2461 tttatgcagg gcgattttgt tgtctaccaa ggccccatta aggataacac cggtaaagaa
     2521 gtaattccgg cgggtaaatc ctacggtcaa aaagatatag cgctggaatc tatggattgg
     2581 ttggtggaag gcgtggtggg gtcggctaag tcataggtgt tctgccgaat gctggtagcc
     2641 tgactggatt aaattgcatt taattcaagc aggctgcgaa tacaatcgac gacccatttc
     2701 agaagttttt taaggcactg caaatctgtt actgaccaca actagctgca ctggtgtttg
     2761 cctgtcagtt attaacccgt cggcttttta tgccgggtta acaactgcgg caaggatgcc
     2821 gcatcggcgg cgcaagccgc cgcgagctcc gctaaaacaa gaaaatccac gcattttcgc
     2881 ca
//
LOCUS       DAOQGS010000544         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21097_length_2881_cov_4.508493, whole genome shotgun sequence.
ACCESSION   DAOQGS010000544 DAOQGS010000000
VERSION     DAOQGS010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21097_length_2881_cov_4.508493"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKZ52_15975"
     CDS             complement(<1..711)
                     /locus_tag="PKZ52_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNG61321.1"
                     /translation="MSRCTLVTIEEAERMIVDMPVLVLDMRDFRAYQRGHFPKSLHLN
                     KDTLRQLIRFTAKQVPILIYCDHGHASQDMVNLFASHGFTNCFSLDGGFDKWFQTVNS
                     PKTPLSFELNNWLAANGFDGGNLDKRGWNNETALICAARQGNYDICAELLEAGATVNA
                     INKDGNNALWMACFSGSDQIADLLIEYDIDVDHQNDNGATALIYAASVGNINIVKHLV
                     YAGADTSLATLDDFTALAV"
     gene            complement(949..1596)
                     /locus_tag="PKZ52_15980"
     CDS             complement(949..1596)
                     /locus_tag="PKZ52_15980"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HNG61322.1"
                     /translation="MSPPVVGALVHSEDMLNQHLVSQVDINSADKILNGGVAPAQWFD
                     KLLGQQRQGRGCLPYFLGLQQADFERILQRVSGQLGPGVMANSLSTGEIFERALLRQE
                     LLELRRDEWLELRNLVNAFAANTEVLQLQLAEIIAAGCLGGDHLWRDLGFTQRSELSE
                     FMLQNFPALAALNTNDMKWKKFFYKQLCELGGGYVCRSPSCEACTAYSDCFGSED"
     gene            complement(1593..2075)
                     /locus_tag="PKZ52_15985"
     CDS             complement(1593..2075)
                     /locus_tag="PKZ52_15985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNG61323.1"
                     /translation="MSTEPLPTLIFYEKPGCASNAKQKAQLKAVGFTLDVRNLLTEAW
                     TAQTLGYYFKSLPVAEWFNKSAPKVKEGLINPADITAQQAFVLMLQEPLLIRRPLIRN
                     AAAADINWVGFDLTLILADLGLPAELASDKPYVSEACTDSSIRCETHADTELKQQEAL
                     "
     gene            complement(2086..2364)
                     /locus_tag="PKZ52_15990"
     CDS             complement(2086..2364)
                     /locus_tag="PKZ52_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNG61324.1"
                     /translation="MALKIVESCVNCFACYDVCPSNAIYQSSPHFKINPKKCTECEDD
                     YSVPQCASICPIEEAILSADGIALNPPGSLTGIPPEKMAEAMREIQAR"
     gene            complement(2448..>2881)
                     /locus_tag="PKZ52_15995"
     CDS             complement(2448..>2881)
                     /locus_tag="PKZ52_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNG61325.1"
                     /translation="MPKPTDTIEKHRRPVLMAVATSGGGLINQHFGHATEFLIYEASP
                     NGVRFISHRKVDQYCVGNDSCGEKESALSGSIRALKGCEAVLCSKVGYEPWSLLEEAG
                     IMPNGEHAMEPIEEAVLAVYKEMIANGKLDEVSPEAQQLTA"
BASE COUNT          758 a          738 c          633 g          752 t
ORIGIN      
        1 aacggccaac gcggtaaaat catctaaagt ggcgagagac gtatcggcac ctgcgtagac
       61 cagatgtttg acaatattaa tattcccaac ggatgcagcg taaattaggg ctgtcgcacc
      121 attgtcattt tgatggtcca catcaatatc gtattcaatc aacaggtcag caatttgatc
      181 tgatccgcta aaacaggcca tccacagtgc attattgcca tctttattaa tagcattgac
      241 cgttgcacct gcttctaata attcagcgca tatatcatag ttcccctgcc gggcggcaca
      301 aatcagggcc gtttcattat tccaaccgcg cttatccagg ttgccaccat caaagccatt
      361 ggcggccaac caattattaa gttcaaaact caacggcgtc ttagggctat taaccgtttg
      421 aaaccactta tcaaaaccac catccaaact aaaacaattg gtaaagccgt ggctggcaaa
      481 taaattcacc atatcttggc tggcatggcc gtgatcgcaa taaatgagga tcggcacctg
      541 cttggcggta aaacggatca actggcgcag agtatctttg tttaaatgca aggatttagg
      601 gaaatggccc cgttggtaag cgcgaaagtc gcgcatatcc aagactaata ccggcatatc
      661 cacaatcata cgttcggctt cttcaatggt aaccagtgtg catcggctca taacgcctcc
      721 tcatctactc aagtatcaag ggtgtcttaa aaatatttat tttccgttct attaacccgg
      781 tggccaaata gatcctcctg atctatttgg ccctcgctcc gctgacactt gcgaaaatgc
      841 gtggattttc ttgttttagc ggagctcgcg gcggcttgtg cctttggcgc ggcatccttg
      901 ccgcagttat taacccggct taaaaagccg ccgggttaat aacagcagtt aatcttcact
      961 tccaaaacaa tcgctatagg cagtgcaggc ttcgcaactg ggtgagcgac acacatagcc
     1021 gccacctaac tcacacaatt gtttataaaa aaatttcttc cacttcatat cgtttgtatt
     1081 caacgccgcg agtgcaggaa aattctgtag cataaattcg cttaactcac tgcgctgggt
     1141 aaaacctaaa tcccgccaca gatggtctcc ccccaaacat cccgcggcaa taatttcggc
     1201 cagctgtagc tgtagcactt ctgtattggc ggcgaaggca ttaaccagat tgcgcagttc
     1261 caaccattca tcccggcgca attccaacaa ctcttgccgc aacaatgcgc gctcaaaaat
     1321 ctccccggtg gataaactat tggccatcac ccctggcccc aactgaccgc tcacgcgctg
     1381 taaaatccgc tcaaaatccg cttgttgcag ccctagaaaa tagggtaaac agccgcgtcc
     1441 ctggcgctgt tggcccaaaa gcttgtcaaa ccactgggca ggcgcaacgc caccatttag
     1501 tattttgtcg gcggaattaa tatcgacctg agacactagg tgctgattta acatatcttc
     1561 actgtgcact agggcaccta caacaggggg gctcataggg cctcctgttg ttttaattca
     1621 gtatctgcat gggtttcaca gcgaatactg ctgtcagtgc aggcttcgct cacatagggt
     1681 ttatcgctgg ctaattctgc gggcaggcct aaatcagcta ggatcagggt taaatcaaaa
     1741 cccacccaat taatatcggc tgcggcggca ttgcgaatga gtggcctgcg aattaacagc
     1801 ggctcctgca gcattaatac aaaggcttgc tgggcggtta tgtctgcagg attaatcaga
     1861 ccctctttta ctttaggtgc acttttatta aaccactcgg ccaccggtag cgacttaaaa
     1921 taataaccga gcgtttgcgc tgtccaagct tcagttaata aattgcgcac atcaagagta
     1981 aagcccaccg ctttaagctg cgccttttgc ttcgcattac tggcgcaacc aggcttttcg
     2041 taaaaaatta aggtgggcaa gggctcagtg gacataaata atggcctagc gggcttgaat
     2101 ttcgcgcatg gcttccgcca ttttttccgg cggtattcca gttaaggagc caggtgggtt
     2161 cagcgctata ccatcggcac ttaggatcgc ttcttcaata gggcaaatgc tggcgcattg
     2221 ggggaccgaa taatcatctt cacattcggt acatttttta ggattaattt tgaaatgcgg
     2281 gctactttgg tagatggcgt tactggggca aacatcgtaa caggcaaaac aatttacgca
     2341 ggattcaaca atttttaaag ccataacaac ctctctttac caggtggtaa atacgcagtg
     2401 cacatcctgc gcactgcgta tcgttgcaga cagaaaacag cgcgtaatta ggcggttaac
     2461 tgttgagctt cagggcttac ctcatcgagt ttgccattgg caatcatttc cttataaaca
     2521 gctaataccg cttcttcgat gggttccatg gcgtgctcgc cattgggcat aatgccggcc
     2581 tcttctaaaa gtgaccaagg ctcataaccc acttttgaac acaatacggc ttcacagcct
     2641 tttaaggcgc gaatactgcc gctcaatgca ctttcttttt cgccgcagga atcattaccc
     2701 acacagtatt gatccacttt gcggtggcta ataaagcgca cgccattagg cgatgcctcg
     2761 taaattaaaa actcagtggc atggccaaag tgttggttaa ttaacccacc gccgctggtg
     2821 gccaccgcca ttaataccgg acgacgatgt ttttcgatgg tatcggtagg ttttggcata
     2881 t
//
LOCUS       DAOQGS010000545         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21129_length_2879_cov_3.929533, whole genome shotgun sequence.
ACCESSION   DAOQGS010000545 DAOQGS010000000
VERSION     DAOQGS010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21129_length_2879_cov_3.929533"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKZ52_16000"
     CDS             complement(<1..219)
                     /locus_tag="PKZ52_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61326.1"
                     /translation="MFPIPPNKPPIIPPKRASGVAPVEKIQERSVPRVTESPTDSERR
                     ERQERRQQARPRIKGYDLAMPGGSEKTFF"
     gene            324..791
                     /locus_tag="PKZ52_16005"
     CDS             324..791
                     /locus_tag="PKZ52_16005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNG61327.1"
                     /translation="MRMISLWFAGFIAWYACASLAIEATSSVKVTPLLKTQTSWDGTA
                     LEYPKGKAEITGMIVEIAPGGETGWHLHPVPSFGMLLEGELEVTLKTGAKKIIKQGEA
                     LAEVVNTLHNGRAISKTAVKILVFYAGSTDSKITEKAPAETKPAATIINSAAK"
     gene            complement(933..>2879)
                     /locus_tag="PKZ52_16010"
     CDS             complement(933..>2879)
                     /locus_tag="PKZ52_16010"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNG61328.1"
                     /translation="PQGAFALSARVLINYADVARTESISPGARVKYTHLLMLKPGAQN
                     ALETALKPQLDAHTRLVTLTNYRSNSQDFIARMNTFLLLAASLGVLLAGVALAIAAQE
                     YTAALAQPFALLKTFGMGPKAVMRVFLLFGAVFFLVAAAVAQGLGVASHLALMLVLKN
                     YLANTELSLTAPILASLLAGLILLLAFLSVPVWQLWRFAPAASLREQKLKTKSALVPG
                     FVAVFGLMGLLTGAWQLSLYLLLGSVVLVLFVALGAGRLIQPITRLGQLWPGVGRAGF
                     LALKRHRKSNRFLLAVFALLLVPIIVMAQLRSRLIDTWQVQLPSDAANYFLFNIYTED
                     KQALVPWLSQQNFNAAPFYPMWRARITAINQRPLAEVIPEEANNPAITREINLTRSTQ
                     LPAGNEIKMGQWWGQEEGELLISVEERIAQMLKLKLADRISLSLAGEVVEAKVASVRA
                     VRWDNLAPNFYLITNKAPQSELTANWITSLYVPPEKRSSLTAQLKQYPNITAIDVAQT
                     LGTFRQLMHKLSQAVELVWVFMGLAGVLVLLASVQASLPARRQEIALLRVAGASKRYA
                     IAGLVGEFFILGLLASVAALVVSEGLLYWLQVRMFKLDYASVLWPWLVVPLFVASLIA
                     FLGGIYCRQLVVLSPATILREQSR"
BASE COUNT          750 a          823 c          686 g          620 t
ORIGIN      
        1 aaaaaaggtt ttttcagaac cacccggcat tgccaggtcg tagcccttga tgcgcgggcg
       61 cgcctgttgg cgccgctcct ggcgctcccg gcgctcgctg tcggtggggg attcggtcac
      121 gcgaggcacg ctgcgctcct gaattttttc cacgggcgca acgccgctag cccgcttagg
      181 cgggataatc gggggtttat tgggaggaat gggaaacata acctggcaca atcctgccgc
      241 gcgacaccct gttatcggct aaaattcgcc gctctttagt gtttcctatc cctatcccgc
      301 cccactctca ataaggccct gttatgcgaa tgatttcttt atggtttgca ggttttatcg
      361 cctggtatgc gtgcgcaagc ctcgccatcg aagccaccag cagcgtaaaa gtaacccccc
      421 tgttaaaaac ccaaaccagc tgggacggaa ctgcccttga ataccccaaa ggcaaagcgg
      481 aaattaccgg catgattgta gaaattgcac cgggcggaga aaccggctgg cacctgcacc
      541 cagtgccctc cttcggcatg ttattagagg gggaattaga agtgacctta aaaaccggcg
      601 ccaaaaaaat tattaaacag ggcgaggcct tagccgaggt ggtgaacact ctgcacaacg
      661 gccgcgctat cagtaaaact gcagtgaaaa ttctggtgtt ttacgcaggc agtaccgata
      721 gcaaaattac tgaaaaagcc cctgccgaaa ccaaacccgc agcgactatt attaactcgg
      781 cggcaaaata gatcgccctg atctattttg ccttcgctgc actaaactta cgaaaatgcg
      841 cagattttct tgttttagtg cagctcgcgg cggtttgcac ctttgacgcg gcatccttgc
      901 cgccgagtta acaatcccgc taaattacct aatcagcggg attgctcgcg caaaatagta
      961 gcaggtgata acaccactaa ttggcggcaa taaataccac ctaaaaacgc gattaggcta
     1021 gccacaaaca acggcaccac caaccagggc cacagcacac tggcataatc caatttaaac
     1081 atacgcactt gcagccaata caacagccct tcactcacca ctaatgccgc tacggacgcc
     1141 agcaaaccga gtataaaaaa ttcccccact aaacctgcta tggcgtaacg cttactggcg
     1201 cctgctacgc gcaaaagtgc aatttcctgc cgacgcgcag gcaagctcgc ctgcacactg
     1261 gccaataaca ccaacacacc ggctaggccc ataaataccc acaccaattc caccgcttgc
     1321 gacagtttgt gcattaattg ccgaaaggtg cccagggttt gcgccacgtc gatggcggta
     1381 atattggggt attgttttag ctgcgccgtt aaactgctgc gcttttcggg cggcacatat
     1441 aaactggtaa tccagttggc ggtgagttcg ctctgcggcg ctttattggt aattaaataa
     1501 aaattgggcg ctaaattatc ccagcgcacg gcgcgaacgc tggccacctt cgcctcaact
     1561 acttcaccgg ccaaactcaa actgatgcga tcggccagtt taagctttag catctgggcg
     1621 atacgctcct ccacggaaat taataattcc ccttcctcct gcccccacca ctggcccatt
     1681 ttaatttcgt tgcccgcagg caattgtgtg ctgcgggtga ggttaatttc ccgcgtgatg
     1741 gcagggttgt tggcctcttc gggaatgact tcagctaagg ggcgctgatt aatcgccgtg
     1801 atgcgcgccc gccacatggg ataaaaaggt gccgcattaa agttctgctg gctcagccaa
     1861 ggcactaggg cttgtttatc ttcggtataa atattaaata aaaaataatt ggccgcatcg
     1921 ctgggcaatt gcacttgcca ggtgtcaatt aagcggctgc gcaattgcgc catgacaata
     1981 atcggcacca ataaaagcgc gaataccgcc aataaaaacc ggttgctttt acggtggcgt
     2041 tttaaggcta aaaaacctgc ccgcccaacg cccggccaca attgccctaa gcgcgtaata
     2101 ggctgaatta atcgccccgc ccccagggcc acaaataaca ccaacactac actgccgagc
     2161 aataaatata ggctgagctg ccaagcgccc gttagcagcc ccatcagccc aaacaccgcc
     2221 acaaaacccg gcaccagcgc gcttttggtt ttgagttttt gctcgcgcaa actggcggcc
     2281 ggggcaaagc gccacagctg ccacaccggc acacttaaaa atgccagcaa taaaattaac
     2341 cccgccaaca ggctcgcaag tataggcgcg gtgaggctca attcggtatt ggctagataa
     2401 ttcttcaaca ccagcatgag cgctaaatgg ctggccactc ctaatccctg cgcaaccgca
     2461 gcggccacta gaaaaaatac cgcaccaaat aataaaaaca cccgcatcac cgctttgggg
     2521 cccataccaa aggtttttaa cagcgcaaac ggctgggcca gggccgccgt atattcctgg
     2581 gcggcaatgg ctagggccac gccagccaat aacacaccta agcttgccgc cagcaataaa
     2641 aaggtattca tgcgggcgat aaaatcctgg ctattggagc ggtaattggt cagcgtcacc
     2701 aaccgggtgt gggcgtctaa ttgtggtttt aaagcggttt ctaaggcgtt ttgcgcaccg
     2761 ggcttaagca ttaataaatg cgtatacttt acccgcgcac cggggctaat actttcggtg
     2821 cgtgccacat cggcataatt aattaatacc cgtgccgaca gcgcaaaagc cccctgagg
//
LOCUS       DAOQGS010000546         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21152_length_2877_cov_3.668675, whole genome shotgun sequence.
ACCESSION   DAOQGS010000546 DAOQGS010000000
VERSION     DAOQGS010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21152_length_2877_cov_3.668675"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..952
                     /locus_tag="PKZ52_16015"
     CDS             470..952
                     /locus_tag="PKZ52_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61329.1"
                     /translation="MNFEEQTPEMKQVFLCASIGEVSMEAHALELQLNLALCLLNSNS
                     DAEFERECKKIRTLSPILDELKAKNIFSARDFKALEEAKHARNDFTHRLSEACVSNIK
                     SGSSKFELIQHFREMKETIRVAMEVAESKMQDIAKDKGLDVNGLVQSAKEHVDKWSLA
                     "
     gene            1079..2107
                     /locus_tag="PKZ52_16020"
     CDS             1079..2107
                     /locus_tag="PKZ52_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61330.1"
                     /translation="MTIEGRTKAAFDWYRQRASELDNEMLGNGGKLPRHELWRRQYYE
                     YPYLLLESNETVLERFADIFTNCLDISADGKITTTPMLENNARLAQYFTEVIEETNWR
                     GILNKASMQPAFIQVNEYYKDGTPLGVEMFKALPLKQEQCLYKFSESGFVREMHKTGR
                     FRISPASYYSKGSHIKAVKDLETERNYRLKAINEVMQGISSIEVEGNTLEIINGIVPL
                     CVVIKDYFLFSTCNELSRRMPTDFEANAVLIIKDKREFLGRLENELLMSYPGWEFLER
                     DVYYYDTYNDHPRDQNQEFYKHISYAYQREHRCILRPKRTGIQEDELKPFFVELGSIE
                     DIAEALYI"
     gene            complement(2404..2832)
                     /locus_tag="PKZ52_16025"
     CDS             complement(2404..2832)
                     /locus_tag="PKZ52_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61331.1"
                     /translation="MIEYDDNWDNIHGLRAKHGYAFTDEYNSLAYFGYQQVTTMLKTW
                     AKRELFNDHAVHLKTLAEEILHSTKAVRVVLGPHQAQQFNGEWYLHITVMSSTGKSYH
                     FFNKFTVYFSKDQYRLEAAFCGASPSATIYEERFRFDYSL"
BASE COUNT          834 a          592 c          694 g          757 t
ORIGIN      
        1 gatgcgcggc gccaggccag ttaaaagcgg tggcgcgggt ttcaaacacg ccttgttcgg
       61 ccattttatt tccagtataa agctctaatc gccgcggccc taaaaccgat attaaccctg
      121 cggcaaaata gatcgtcctg atctattttg ccttcgctgc actaaaagtg gcgaaaatgc
      181 gcggattttc ttgttttagt gcagctcgcg gcggtgtaca ccgccgaggc ggcatccatg
      241 ccgccattat taacccggca ctaattaccg ccgggttaat aacacagcta cgcgccgtgt
      301 tatctaaaat taggtgcgca aacggtctcg ctccggttga tggctgcttt ttgaaacgct
      361 tagagggttg gttctgggtg gtggacttaa gggtgtgata gacggcgagg aaatatgtaa
      421 tatgcggcga gtgccgtgca aaaagctgat gagcaaatcg aggtgcagcg tgaattttga
      481 agaacagaca ccagaaatga agcaagtatt tctgtgcgcc tctataggtg aggtatcaat
      541 ggaggcgcac gccttggaac tacagctaaa tctagctctt tgcctgctta attcaaatag
      601 cgatgcggag tttgagcgcg agtgcaaaaa aatacgtaca ttgagcccca tacttgatga
      661 gcttaaggcg aagaatatat tttccgcccg ggacttcaaa gcacttgaag aagcaaagca
      721 tgctagaaat gactttactc atcgcttgtc cgaagcgtgc gtgtcaaata taaagtcggg
      781 atcgtcaaag ttcgaactta ttcagcattt tcgtgagatg aaagaaacta tacgagtggc
      841 catggaggtg gccgaatcga aaatgcaaga tattgccaaa gacaaagggc ttgatgtaaa
      901 tggtctggtg caatctgcga aagaacatgt ggacaaatgg agcttagcct aattgtgcaa
      961 ggaggtctga ctgcttttcc gttccttgtt ctgtggttta acgctacgct accgcaaaac
     1021 aattcactac aaagccgcag ctgcttgcgg cgttagctat aaaatatcgg taagaaaagt
     1081 gacaatagaa gggcgtacca aagctgcatt tgattggtat cggcagagag cttccgagct
     1141 ggataatgaa atgcttggaa atggcggaaa attgcctagg cacgaattat ggcggcgtca
     1201 atattatgaa tacccatact tgctgctcga atcaaatgag accgtattgg aaagattcgc
     1261 ggatatattt acaaactgct tagacatttc ggctgacggg aaaataacaa cgaccccaat
     1321 gctagagaat aatgcacgtt tggcacaata ttttacagaa gtcattgagg agaccaactg
     1381 gcgaggaatc ttaaataagg cttcaatgca gcctgcattt attcaggtca atgagtatta
     1441 taaagatgga acacctctag gtgtcgaaat gttcaaagcg ttgcccttaa agcaagagca
     1501 gtgcttgtat aagttttcag aatcaggctt cgttcgcgaa atgcataaaa caggaaggtt
     1561 tcgcatttct ccagcttcat attactcaaa gggaagtcat ataaaggcgg taaaagatct
     1621 tgagacagaa agaaactatc gcttgaaggc cattaacgag gttatgcaag gtatttcatc
     1681 aatagaagta gagggtaaca cgcttgaaat aataaacggt atcgttcctc tttgtgttgt
     1741 tataaaggac tattttctat ttagcacttg caatgagctt agcaggagaa tgcctacaga
     1801 ctttgaagct aacgcagtat tgataataaa agacaagcgc gaatttcttg gccgtttgga
     1861 aaatgagctc ctcatgagct acccaggttg ggagtttttg gagcgtgatg tttattacta
     1921 tgatacctat aacgatcacc cgagagatca aaatcaagaa ttttacaaac atatatcata
     1981 tgcttatcaa agagagcatc ggtgcatact taggccaaag cgtacaggta tccaagaaga
     2041 tgaactaaag ccattttttg ttgaactggg gtcgattgaa gatatagccg aggctttata
     2101 tatatgaaga gtttgtgcgt aaagataaca atcgtagcca ggccgaaatt tattccaacg
     2161 ctcgtttcgt gccgaaaaag ccagggcggg ttaaaacccg cggggttcga tatcatcgcc
     2221 tgtgcaaacc tcttcattaa attttcattg gttggctttt aataaagctt acgtggcggt
     2281 cgcattgtgc tgcgcgggtt acaacccgca ctacgtaagt cacatgcttt ttagccacca
     2341 tagcctaact gccaattgaa tacttttccc aatcactcaa taaaccgcca ggccagataa
     2401 aacctacagg ctgtaatcaa accgaaagcg ttcctcataa atcgtggcgc tgggcgatgc
     2461 gccgcaaaaa gccgcttcca gcctatactg atccttcgag aaataaaccg taaatttatt
     2521 aaaaaaatgg taagacttgc cggtgctgct cattacagta atgtgcaaat accattcgcc
     2581 gttaaattgt tgggcttgat gggggcctaa taccacgcgt actgccttag ttgagtgcag
     2641 gatttcttct gccaaggttt tgaggtgcac ggcgtgatca ttaaatagtt ctcgcttggc
     2701 ccaggtcttg agcatggtcg tcacctgttg atagccaaaa taggctaagg aattatattc
     2761 gtccgtaaag gcgtagccgt gtttggctcg cagcccgtgt atgttgtccc agttatcatc
     2821 atattcaatc ataaatagag tctccgcgtg aggtgctacc ctcggttggg ttgaggc
//
LOCUS       DAOQGS010000547         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21190_length_2874_cov_4.976233, whole genome shotgun sequence.
ACCESSION   DAOQGS010000547 DAOQGS010000000
VERSION     DAOQGS010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21190_length_2874_cov_4.976233"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(628..1857)
                     /locus_tag="PKZ52_16030"
     CDS             complement(628..1857)
                     /locus_tag="PKZ52_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG61332.1"
                     /translation="MSQHEKTIQETIQADRRRWLSLLAKGGALASMARTSALAAAVLA
                     NRLAEAQVAPSKKFILIYHPGGAPRNYLTSIAVRPFEPFGATVAALTMTINTPGNHGL
                     IFRAAGANSFNPLELNSSTIDQQIADTIGHLTPLRSVELGVLSGNYEGFIRYKGQAVP
                     RINSPEAALQRYWRELPTGTGLSSYERRKLMLTAHQQGLDKILSQLSLEERFKLEAHV
                     GALERYQQRLDANVQQGCGTVAYTKGETPLNLYRAQGDIAVAALACGLTNVASIQFNS
                     TYEDWSPNDGTADGVQAVGDHALILNGGNYAALGPAVNEYMNKGVAHIINKLIAAGIY
                     QDTVVLCLTEMGDSSNNTPDAGPITMATGMPGFKGGLRRLNNDHYEIFPDVVRLMGLE
                     WAVGQTIYKYSGGGLVV"
     gene            2623..2838
                     /locus_tag="PKZ52_16035"
     CDS             2623..2838
                     /locus_tag="PKZ52_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61333.1"
                     /translation="MGIRHRMSHLCMGFTLTCVFLSALCANAASSSSSASSTSSSASS
                     SSSSGGILATSPPPNSSSGGLREPLKN"
BASE COUNT          754 a          747 c          663 g          710 t
ORIGIN      
        1 ttcgccaaat ttaacgccgc cagagctagc aaggtagtat ttcaacaagc tgctaaatgc
       61 cgccgggtta ataaaacacg aatctctatt ttcggcacaa cattctgttt atgtgtttat
      121 ggtcgcaacc tgttgcgatc gtaaacacaa ctccctaaaa acactccata aaggccaaaa
      181 gcgcttaaac ctaatggctg ccacaatgca gccgactgcc ctaaaaaccc tgttttgctg
      241 acaaaaaata gcgctaacaa caaccaaacc acactgtggc cccagcgcaa tattaaatgc
      301 aaaatcagcg ggcgagcgcc tgcatgggcg gcattgaggg gtttgggcca tagtatgtaa
      361 taaatcgccg ctattaaaat tgcgcctatg cttgcggccc aataaaaatt ctcggccatg
      421 aaaaacctct tgagcgattg gataagccgc gctgaccatt ttatattggc tgcgccaccc
      481 aaaatatttg ctgcgttagg cgcaccttgg tgcgcctaat aagcacagag ccacagtatg
      541 agtttaaatt ttaccgatgg aatgagtgaa tttgacgcac ccagatatcc gcaacgaatt
      601 cgtttagggt aattaattat aaagaaacta aaccaccaag ccgccgccag aatatttata
      661 aatcgtctgc cccaccgccc actccagccc cattaaacgc accacatccg ggaaaatttc
      721 ataatgatcg ttattgagcc tgcgcaggcc gcctttaaat ccgggcatgc ctgtagccat
      781 tgtaattggc cctgcatccg gcgtgttatt actgctatcg cccatctcag tcaggcataa
      841 aaccactgta tcctgataaa tacccgccgc tattaattta ttaataatat gcgccacgcc
      901 tttgttcata tattcgttta cggcaggccc cagggctgca taattgccgc cattgagtat
      961 tagggcgtga tcccccaccg cctgcactcc atcagccgtg ccatcattgg gcgaccaatc
     1021 ttcataggtg ctgttaaatt gtattgaggc aacattggtt aaaccgcagg ccaaggccgc
     1081 aacagcaata tccccctggg cgcggtataa atttaatggg gtctcgcctt tggtgtaggc
     1141 tactgtgccg caaccctgtt gtacgttggc atccaagcgt tgctggtagc gctccagcgc
     1201 acccacgtgg gcctccaatt taaaacgctc ttctaaactt aattggctga gtattttatc
     1261 caggccctgt tggtgcgccg tcagcatcag cttgcgccgc tcgtagctac tcaaacccgt
     1321 gcccgtgggc aattcccgcc agtagcgctg cagggccgcc tcgggtgaat taatgcgggg
     1381 caccgcttgg cccttgtagc gaataaaacc ctcgtaattc ccgcttaata cacccagttc
     1441 aacggagcgc agcggtgtta agtgcccaat agtatcggca atttgctgat caatcgtgct
     1501 ggagtttaat tccagcggat taaatgaatt tgccccggcc gccctaaata taaggccgtg
     1561 gttgccgggt gtgttgatgg tcatggtcaa tgccgcaacc gtagctccaa acggctcaaa
     1621 cggccgcacc gcaattgatg ttaaataatt acgcggcgcg ccgccggggt gataaatcaa
     1681 aatgaatttt ttgctagggg ccacctgcgc ctcggccagg cgattggcca acaccgcagc
     1741 agcaagcgca gaggtgcgcg ccatactggc aagagcgccc cccttggcta aaaggctcaa
     1801 ccaacggcgg cggtcagctt gtattgtttc ttgaatggtt ttctcgtgtt ggctcataac
     1861 agactccact tcattatgtt taaccaaaga ttgcgcttgg cattcctacc caactgcact
     1921 ggggtttaca aacagcaacc tgtggttgtg ggtgctgcaa atattgaggg ggataatata
     1981 agcaactggc ggtggggtaa aaagtatcgg cgcctaatgt gatgatggcc gcaatgggtg
     2041 tgtgcaatta caccatgaga atatttatct gttttatata taggggcttt gttagcagcc
     2101 cgggcagctg cttttaattg cgggccatac acaacccgcc tagcttttag cgcaattaga
     2161 aatggtaggc tgcaatgggt gcgcacacta aaagcccgcg caaagctcgg aacttttatt
     2221 aacccggcac aaaggaacct ctgaaaaata tcggcggcga agcactggct aggcgaattt
     2281 gccaaaaaaa gcgcagttta cacacagtaa atgagcatct ttttggcaaa ttcaacgacg
     2341 ccagagcgag taaggtaatt tttcagaggc tccttaaatg ccgccgggtt aaattgtgtt
     2401 gtgcgcgata ttttattcgg cgctaggggt aatgatttct gcataccaca cattgccgca
     2461 cgcgaactca acaccgagaa attcatcaca tacaatcacg tatttcgccg caaaaccctc
     2521 aatctacgta aatttacgtg catacaaaca gtgcagctgg ctgctaaggt cttgagtgta
     2581 ttgtctcagc cgccattttg tagaccaaag agaggatctt atatgggaat tcgtcatcgc
     2641 atgagccatt tatgcatggg gtttacacta acctgtgtat ttttatcagc gctgtgtgcc
     2701 aacgcagcct caagctcatc cagcgctagc tccacatcca gtagcgcatc atccagttca
     2761 tcaagcgggg gcatactggc tacatctccg ccgccaaatt catcctccgg cgggcttagg
     2821 gaacctctga aaaattaatc tttcttaaga aagcccccca aaaaatcgta gatt
//
LOCUS       DAOQGS010000548         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21322_length_2862_cov_4.401140, whole genome shotgun sequence.
ACCESSION   DAOQGS010000548 DAOQGS010000000
VERSION     DAOQGS010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21322_length_2862_cov_4.401140"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..883
                     /locus_tag="PKZ52_16040"
     CDS             389..883
                     /locus_tag="PKZ52_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNG61334.1"
                     /translation="MSAIIINDLCAKDIMTRDLLTAYEGWTIHRLADFFINRNISAAP
                     VIASDHELVGVVSVDDVCRFNNLNEQDKSNALRNHYRDSCGQDINEEVLRSWVKDAAK
                     NCTVHQIMTHEVIAVDQGEPVYAVAKLLSDRHLHRVFVTEDNKVVGVITVMDVLRAFV
                     AAGA"
     gene            complement(1049..1678)
                     /locus_tag="PKZ52_16045"
     CDS             complement(1049..1678)
                     /locus_tag="PKZ52_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNG61335.1"
                     /translation="MSQADTRTLLLDTALALIWRSNYDSVGVNEICKQAGVTKGSFYH
                     HFESKLELFFEASNHYWETIRKDLDGLLSPINPPLEQLAKWITFIFINKVGKDEENIP
                     GCAFFSACMQIGAGDPRIISSLQAMLERGATYNRALIQNLHNGNYLDDEVNCEQKARL
                     MQQFVHGAITYVRASKNIANLKSDLPKGIFRLIGLKKEYWEAVESQIAI"
     gene            1998..>2862
                     /locus_tag="PKZ52_16050"
     CDS             1998..>2862
                     /locus_tag="PKZ52_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG61336.1"
                     /translation="MKASAALFKKSTALLAVALMSTSGLFWFGQQSQANAANGAPPPP
                     PSVEVVELKPTPVRSWVDFSGRLAAVESASLKPQVSGIIQKILFTEGQEVRQGQPLLV
                     IDPRPHEAALQQAKAKLASAESQYQLARDELKRAQQLLKQKLIADSLFEASVNAEAVA
                     AASRQEAQAAVARAQLNVEYAHISAPISGRISRAELTQGNNVEAGANAPVLARITASK
                     TLYAEFNVDEASYIKLVRKTQVLRDMPVQVRLAAEQADAYGGHLESIDNHIDPATGTI
                     RARAILDNAKGE"
BASE COUNT          661 a          666 c          759 g          776 t
ORIGIN      
        1 gctggcttct ttttctagcc gggggctgtg atgcacttcg gagttgaatc tgccttcacg
       61 acgcatggcg caaaacgcat aaaccgagtt gggtggctat cgcgtggcat gcttatgtgt
      121 agattgtcct gttatatatg tgaaccgggt tcgcttcagg gggctggcgt tgcttgggtt
      181 attcggctac ttttatctgg ttgccgtgct tagtctcagc ttgttaccgg ttcgacaatt
      241 cggttgcggt atctagcagc gtgttgaaat actaccttgc cagcgcgtcg ccgcctaggt
      301 atttcaacaa gctgctagtg ggcaaggctg ggttatctgt ctgtcattgc ctgtcgatta
      361 ttcctgctgt gcctttgaga gatcatttat gagtgcaata ataattaacg atttgtgcgc
      421 aaaagatatt atgactcgcg atctgttaac cgcctatgag gggtggacaa ttcatcgctt
      481 ggcggatttt tttatcaatc gcaatatatc cgctgcccct gtcattgcgt cggaccatga
      541 gttggtgggg gttgtgagtg tggatgatgt gtgccgcttt aataacctca atgaacagga
      601 taaaagcaat gcgctgcgca atcattaccg agatagttgt gggcaggata ttaatgagga
      661 ggttctgcgc tcctgggtga aggatgcggc aaaaaactgc actgtgcatc agatcatgac
      721 ccatgaagtt attgccgttg accaaggtga acctgtttac gctgtggcta agttgttatc
      781 tgaccgccac ctgcatcggg tatttgtcac tgaggataat aaagtggtgg gggttataac
      841 ggtgatggat gtactgcggg cgtttgtggc ggcgggcgct tagtgttcaa taggttgctt
      901 gatactgtgc ttgcaattac aaccggtgca agatcagccg gttgcacctg tttacaggca
      961 tatacaaatt aagcttgaat aattttggct gccgatctgc tgtgggctgg tcgtccggct
     1021 gcgaattttc tatttcacta tttgctattt aaatagcaat ctggctctct accgcttccc
     1081 aatattcttt tttcaggcct attaaacgga atattccttt ggggaggtca ctttttaagt
     1141 tggcaatatt tttggaggca cgcacatagg tgatggcccc gtgcacaaac tgctgcataa
     1201 ggcgggcttt ttgctcgcag tttacctcgt cgtctaaata attgccgttg tgcaggtttt
     1261 gaatgagcgc gcggttatag gtggcgccgc gttctaacat ggcttgcaat gagctgataa
     1321 tgcgcgggtc gcccgcgcca atttgcatgc acgcagaaaa aaatgcgcag ccgggaatat
     1381 tctcttcgtc tttacccacc ttattaataa aaataaacgt aatccatttg gctaattgtt
     1441 ctagtggggg attgatgggg gataagagcc cgtcgagatc cttgcggatg gtttcccagt
     1501 agtggttgct ggcctcaaaa aacaattcca atttagattc gaagtggtgg taaaaactgc
     1561 ccttggtaac gcccgcttgt ttacagattt cgtttacccc caccgagtcg taattgctgc
     1621 gccaaattaa agctagggca gtgtccaaca atagggtgcg ggtatcggct tggctcatgg
     1681 tggttctcgt ggctgttttg ggttggggtc tacttctgta ccaactggta tgtatgctgc
     1741 ttgggcgata agctagtcac ccattggcgc ctagggtagc tatagcgcta ggttctcgca
     1801 atctgttcct aagtgtaggg cattttttca caggcttttt gtttaatatc acaaatcctc
     1861 cccgcttaag tggttattcc agccaggccc aaccataaaa ataaatcctt gaccaacata
     1921 ccaaccggta tgtatgctta ctgaaagcgg acatctcggt gtccttatgg ttcccacctg
     1981 gtatgagagc agaaggtatg aaagcgtcag ctgcgttatt caaaaaatcc accgccctgt
     2041 tagcggttgc cctgatgagt acttccggcc tgttttggtt tggtcagcag tcgcaggcaa
     2101 acgccgccaa tggtgccccg ccgccgcccc ccagcgttga ggtagtggag ttaaaaccca
     2161 cgcccgtgcg cagttgggtt gatttttccg ggcgcttagc cgctgtggaa agcgccagcc
     2221 ttaagccgca ggtgagcggt attattcaaa aaatattatt caccgaaggg caggaggtgc
     2281 gccaaggcca gcccctcttg gtgatcgacc cgcgccccca tgaagcggcg ctgcaacaag
     2341 ccaaagccaa attagccagc gccgaatccc aatatcaatt ggcccgcgac gaattaaaac
     2401 gcgcgcaaca attgctcaaa caaaaattaa ttgccgatag cctatttgaa gccagtgtta
     2461 atgccgaggc agtggcggcc gcaagccgcc aagaagcgca agctgcagtg gcccgcgcac
     2521 agttaaatgt tgaatatgcc catatttcag cgccgattag cggccgcatc agccgcgcgg
     2581 aattaaccca gggcaataat gttgaggccg gcgctaatgc gccagtatta gcgcgcatca
     2641 ccgcaagcaa aactctttat gctgaattta atgtggatga agccagctac attaaattag
     2701 tgcgcaaaac ccaagtgttg cgtgatatgc cggtgcaagt gcgccttgcg gctgaacaag
     2761 ctgatgccta tggagggcat ttagaatcca tcgataatca cattgacccg gccacgggca
     2821 ccatccgcgc gcgggctatt ttggataatg ccaaaggcga at
//
LOCUS       DAOQGS010000549         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21329_length_2862_cov_3.573566, whole genome shotgun sequence.
ACCESSION   DAOQGS010000549 DAOQGS010000000
VERSION     DAOQGS010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21329_length_2862_cov_3.573566"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1370
                     /locus_tag="PKZ52_16055"
     CDS             96..1370
                     /locus_tag="PKZ52_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61337.1"
                     /translation="MNDAEYLTQLLGYLPPPIFRAFLQNEFNYSLPLLDDKTPYKQQR
                     RAFFTALSQLESSCRQHMDDSAESIIVLSDECGQRVLEGFARDLCDESRFHALTTLPD
                     QYQRALWLYTHERALFNEALQTRQADIVRTNITCYSGFKAPKNAPVLDDIEAREMLQH
                     EVARYLECPATGVAVQIFKRLRSDSEASQAVDIVHIRIYHNSHPILVDCVHNGELVSR
                     RVIRAELSHITYEPANGHLEVTTQSTLSRENLARLVIRVLMPSEPPVPAMPSHRTLST
                     DPVHQRVIVKRYDYQRLSTPTIFDISEEPIAYTKVIELGYSDLANRTLLIKINYQDVD
                     DIYTASKSLINPVFDFSQYPLNYAKLAVRLKRTDRERGRTISIILRGDNECNIKSKHD
                     RDRALCDRLLHKWRLIKDRKDRECSAHDYVAA"
     gene            1336..2334
                     /locus_tag="PKZ52_16060"
     CDS             1336..2334
                     /locus_tag="PKZ52_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61338.1"
                     /translation="MNAPLTITSLLELIRLFEYASTFLDDQGQRLVGVPNWALTQSSL
                     SCEERANWLVPIGYTNVYPMTVDQNLMFADVTYDTDSNQYYYRSAKSFRKCSVNMDDL
                     AITAISEEKLLHYLADLCGVPVALRTGIQSPAITDVLWYLGKTRIRETWIDIWCARHL
                     SAKSALIFQHLKNKHLPDKGLVLTFGAVLPEVIPPPRQYHFLALKQVLCPRGNGYGID
                     GDLLHRFLGGTSPQDTDSMLPVRFDAYSNTLVITTKVIAPWVIKGTRQQAVVRYLFEQ
                     LHKDRQWVASHEILSYVYGAQQQGHSRRISDIFRGNPLWKDYITTSAKGLYGFKLD"
     gene            2607..2816
                     /locus_tag="PKZ52_16065"
     CDS             2607..2816
                     /locus_tag="PKZ52_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG61339.1"
                     /translation="MSVKHLNQSQLAERWGVSESTLERWRTDGIGPVFLKLQGRVLYR
                     VEDIHAYEADCLRKSTSESATGSER"
BASE COUNT          711 a          718 c          689 g          744 t
ORIGIN      
        1 atttcaatca gttagcacgc gaaggagtct ggcaggttgc tctatggagg tagagaaagt
       61 cgttctttcg ctagataaga tttagtgagg taaccatgaa cgacgcagaa tatttaactc
      121 aacttttggg ctatttgccg cccccaatat ttcgcgcttt tttgcagaat gaatttaatt
      181 attcccttcc cttattggat gataaaacgc cctataaaca gcagcgcagg gcatttttca
      241 ctgcgttaag ccagctagag agttcctgcc gccaacacat ggacgatagt gcggaatcga
      301 ttattgtgtt atcagatgag tgcggacaac gagtgctcga gggtttcgca cgcgatctct
      361 gcgatgaatc tcgctttcat gcgttaacga ctttaccgga tcaatatcag cgggcattgt
      421 ggctgtacac acatgagcgc gccttattta atgaagcact ccaaacacgg caagcggata
      481 tagtacgcac caatatcact tgttattctg ggtttaaagc gcccaaaaat gcacctgttt
      541 tagacgatat tgaagcgcga gaaatgcttc agcacgaggt tgcccggtat ttggagtgcc
      601 ctgcgacagg tgtcgctgta cagattttca agcgcttgcg ctcagacagc gaggcaagcc
      661 aggcggtaga tattgttcac atccgtattt accataattc tcacccgatt ttggtggatt
      721 gcgtccataa cggtgagctt gtttctcgac gcgtcatccg cgcggagctg tcgcatatta
      781 cctacgaacc ggcaaatggc cacctggaag tcaccaccca atcgaccttg agtcgagaaa
      841 acctggcccg cttggtgatc cgtgttttaa tgccgtcgga accgccggtg cctgcaatgc
      901 cgtctcatcg tacgctgtcg acggatcctg tccaccagcg agtaatagtg aaacgctatg
      961 attaccaacg gttatcaaca cctactattt tcgatatttc cgaagaaccc atcgcctata
     1021 ctaaggtgat tgaattaggt tattcagatt tagccaatcg caccttatta attaaaatta
     1081 attaccaaga tgtcgacgat atttataccg cttcaaaatc attgatcaat ccagtcttcg
     1141 acttcagcca gtatccctta aattacgcca agttggcggt tcgcctaaaa cgaactgatc
     1201 gagagcgtgg gcgcacaatc tccatcattt tgcgcggcga caacgagtgc aatattaaat
     1261 ccaaacatga tcgagaccgc gcactctgcg accggttgtt gcataaatgg cgcttgatta
     1321 aggacagaaa agaccgtgaa tgctccgctc acgattacgt cgctgcttga attaattcgc
     1381 ttatttgaat acgcatccac atttttagat gaccaaggac aacgcctggt aggggtgcct
     1441 aattgggcgc tgacccagtc gagtttatca tgtgaagagc gtgccaactg gttagtacct
     1501 atcggttata ccaatgtcta tccgatgacc gtggatcaaa atttaatgtt cgcggatgta
     1561 acctacgata ctgattccaa ccaatattat tatcgatctg cgaaatcatt tcgcaaatgc
     1621 tcggtgaata tggatgacct ggccattacc gcaatatcgg aagaaaaatt attgcattat
     1681 ctggcggatt tatgtggtgt acctgtggca ttgcgtactg gaattcagtc accggctatt
     1741 accgatgtac tctggtattt aggtaaaacc cgtattcgcg agacgtggat tgatatctgg
     1801 tgcgcgcggc atttatcggc aaaatccgct ctgatttttc agcacctgaa aaataaacat
     1861 ctcccagata aagggttagt tctgaccttc ggcgcggttt tgccagaggt gattccaccg
     1921 ccgcgccagt atcatttttt agcgttaaag caggtgttgt gtccacgtgg aaatgggtac
     1981 ggcatcgatg gggatttact acatcgcttt ctcggtggca cctccccaca agataccgat
     2041 agcatgcttc cagtgcgatt tgatgcgtac agtaatacgt tggtgatcac cacaaaagta
     2101 atcgccccat gggtcatcaa agggactcgc caacaagccg tggtgcgtta cttgttcgag
     2161 cagctgcata aggatcgcca gtgggtcgcc agccatgaga ttttgagtta cgtgtacggc
     2221 gcgcagcaac agggccacag ccgtcggatc agtgatattt ttcggggcaa cccgctgtgg
     2281 aaagactaca tcacgacgtc cgcaaaaggc ctgtacgggt ttaagttgga ctgatcgtcg
     2341 gcacatctgc tcgtcgtctg ctcgtcgcta agcagccttc cctataaata gttttctttg
     2401 cgcagccgcc ctattgaacc gcctccgggc ggttttttgt gttcaggaat tagcacagcg
     2461 tgtgatgagt tcgcacgccc cccgtttaat tactcatccc ttcgcttaaa ccctcccata
     2521 tccccaccca ttagcggggc agacgccccg ccatcccagt ttggaaactg cgctcacgca
     2581 tcctcaataa cccataggag cacatcgtga gtgttaaaca tttgaatcaa tcccaattgg
     2641 ctgagcgctg gggcgtcagt gagtcgaccc tggagcgctg gcgaaccgac ggcatcggcc
     2701 cggtttttct taaattgcaa gggcgggtgc tctaccgcgt ggaggatatt catgcctatg
     2761 aagcggactg cctgcgcaaa agcacctcgg aaagtgcaac cgggagtgag cgatgatgct
     2821 gacgctgaac caagccttgg acctctccgc ccacgacctc gc
//
LOCUS       DAOQGS010000550         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21361_length_2859_cov_5.869472, whole genome shotgun sequence.
ACCESSION   DAOQGS010000550 DAOQGS010000000
VERSION     DAOQGS010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21361_length_2859_cov_5.869472"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="PKZ52_16070"
     CDS             <1..2206
                     /locus_tag="PKZ52_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61340.1"
                     /translation="SVLVGFSITAMGLALMFNINNAQTKQVTAQAQVNAQSIAWASAE
                     AFRQVLQGMPSGELTQLVPGAEFRPPAGAQPNRVTPTVVFGLQPVPVNGTVPAHSELT
                     MNLIATDPAAQVGTTLQMVYGIFGLGPPTLKEDGAAAFYDNARFEGEVKYKNVESGSE
                     RVSVKGNVQFTSKISGVSAVRATGDVTLNNNEVVLQSVYANGTVTMQGDAKVVEKLVG
                     IAGVNIQQGFAGSIYSDGNVLYRTAAPATGTSAIVTQVAARGSVDITNQNNLEFRDIG
                     SMAATTLAGCTTGTCFKYVESEGVLKADPLRLERGYSKARITCDSSAQPGSEAVAPAF
                     TGCSLTAPTKFRAATPPLKPIPPLQPIDLEPPLIDVWEREEHANYVVRYDGARSIITV
                     KNVGRGNVTLAGQYELVRSGAQGFLCLLDASGVRDAGCTAAAAPKNIFCTDGCWSVQG
                     GGNQAGAAPYTFYLEGHVAPGVILFDGNLNMKMTPLSPAAFLAAGYIRTEGNIGRSLA
                     LNFAGPDGGAVNGVTLPGVCRNLANAAINLLPTNFCTASGYESTAADNLGNVTLMAGG
                     QRRTSITRTTASYQTGDVDSTSSQETAATGGRVGKIVTKKIVDKNAGTTVTEVTTLAP
                     YSGGDIQLAAENVVYGSILAGNLFRTEGKTTLYGYVVSSALAKKYAATGGGVIYNDGG
                     LGLTQNWLTAETTIDHSVIPEYYKPAEIRGGGGGGGGNGAGVSVQVMRARYL"
     gene            complement(2343..>2859)
                     /locus_tag="PKZ52_16075"
     CDS             complement(2343..>2859)
                     /locus_tag="PKZ52_16075"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNG61341.1"
                     /translation="VVERLIQPKATEGFKETFGKFTASLAIQEREKLGADVNTRIIQN
                     IEGPVVITSVQIENIDFSSEYEHSVEERMLAQVLVEKEKQNLAKERVLAEIKVTQAKA
                     QADAIEAQARAEAEAIRLKGEAEAKAITARSEALKNNAALIELVTAEKWDGKLPSTML
                     PNATVPFISTK"
BASE COUNT          649 a          715 c          775 g          720 t
ORIGIN      
        1 gtcggtattg gtgggtttta gcatcactgc aatggggctg gcgttgatgt tcaacataaa
       61 caacgcccag accaaacagg tcaccgccca agctcaagtt aatgcgcaaa gtatcgcttg
      121 ggccagcgct gaagcgtttc gccaggtgct ccaaggtatg ccctctgggg agctgaccca
      181 actggtgccg ggtgcagagt tccgtcctcc ggctggcgca caaccaaaca gagtaacgcc
      241 aacagtagtc tttgggctgc agccggtccc agtgaacggc acggttcctg cccatagtga
      301 gctaaccatg aacctcatcg ccactgaccc ggctgcccag gtgggcacaa ccctgcaaat
      361 ggtctatggc atttttggtt tggggccacc gacattaaaa gaagatggag ccgcagcctt
      421 ctacgacaat gcccgctttg aaggggaggt taaatataag aatgttgagt ccggcagcga
      481 gcgagtatcg gtcaagggta atgtgcaatt caccagcaaa ataagcggtg taagcgctgt
      541 tcgcgccaca ggcgatgtga cgctcaataa taacgaggtt gtattgcaga gtgtttatgc
      601 caacggcacg gtaactatgc agggcgacgc caaggtggtg gaaaaacttg tcggtatcgc
      661 aggcgtgaat attcagcaag gttttgcagg cagcatatat tcggatggca atgtgctgta
      721 tcgcaccgcg gcacccgcta caggcactag cgctattgtc acccaggttg ctgctcgtgg
      781 ctcggtcgat attaccaatc aaaacaatct ggagtttcgc gatattggca gcatggccgc
      841 cacgaccttg gcgggctgta ccactggtac ctgttttaaa tatgtggaat ctgagggtgt
      901 acttaaggcc gatcctttgc gcctagagcg cggctacagc aaagccagaa tcacctgcga
      961 ctccagcgct caacctgggt cagaagctgt tgcccctgcc tttaccggct gttctctaac
     1021 ggcacctacc aagttcagag ctgcgacgcc gcctcttaag cccatccccc ctttgcaacc
     1081 catcgacctc gagccgccgc tcattgatgt ctgggagcgg gaagagcacg caaactatgt
     1141 cgtgcgctac gatggcgctc gtagcatcat cacagttaaa aatgtcggcc gaggcaatgt
     1201 gaccttagcg gggcaatatg agttggtgcg ctctggtgcc caggggtttt tatgtttgct
     1261 ggatgccagc ggggttagag atgcgggttg taccgcagcg gccgctccta aaaatatttt
     1321 ctgcaccgat ggctgttgga gtgtgcaggg cggaggcaac caggcgggtg cggcgcccta
     1381 tactttttat ctagaaggcc atgtggcacc cggcgtgatt ttatttgacg gcaatttaaa
     1441 tatgaaaatg acgcccctct caccggctgc ctttttggcc gcagggtata tccgcacgga
     1501 gggcaatata ggccgcagtc tcgcccttaa ctttgctggc cccgacggag gtgcggtaaa
     1561 tggtgtcacc ttgccaggag tttgtagaaa cctagcaaat gcagcgatta atttgctgcc
     1621 tactaatttt tgtaccgcca gtggttatga atccaccgcc gccgataatc tgggcaatgt
     1681 aacccttatg gcgggcgggc agcgccgaac tagcattact cgcactacgg caagctatca
     1741 aacgggcgat gttgattcca ctagcagtca agaaactgcg gctacaggtg ggagggttgg
     1801 caaaattgtg accaaaaaaa ttgtcgataa aaacgcaggt accacagtca ctgaagtgac
     1861 cactctggcg ccctacagcg gtggtgatat tcagctggcg gcagagaatg ttgtttacgg
     1921 ctcgatattg gccggtaatc tgttccgcac tgaaggtaaa accactcttt acggctatgt
     1981 agtttcatcg gctttggcca agaaatacgc cgcgaccggt ggtggagtca tttataacga
     2041 tggcggattg ggtttaacac aaaattggct tactgccgaa actacaattg accatagtgt
     2101 aatacctgag tattacaagc ctgcggaaat ccgaggcggc ggtggcggcg gtggcggcaa
     2161 tggtgctggt gtcagcgtgc aagttatgcg cgctcgctat ctctaattat tttggtttaa
     2221 atgtttcatc ggtaaagtcc cggtgcgcgc atactctagc tttgaatctt aatttgatta
     2281 aatagcgcgc tcttttaccg ggatgtaatt gtgtagaaag gcgcctgtaa caagatatct
     2341 agttatttgg tcgaaataaa aggcaccgtt gcattaggca acatggtgct cggcaatttt
     2401 ccgtcccatt tctccgccgt taccaactca attaacgccg cgttattttt taatgcctca
     2461 ctacgggcgg tgatggcttt tgcttccgcc tcgcctttta aacgaatggc ttctgcttcg
     2521 gcccgggctt gggcttctat ggcatctgcc tgcgcctttg cttgggtgac tttaatttcc
     2581 gctaagacgc gctctttggc caggttttgt ttttcttttt ccaccaatac ttgcgcgagc
     2641 atgcgctcct ccactgagtg ctcgtattcg gacgagaaat cgatattctc gatttgcacc
     2701 gaagtaataa ccactggccc ttcgatattc tgaataatgc gcgtattgac atctgcgccg
     2761 agtttttcgc gttcctgaat ggcgagactg gcggtaaatt tgccgaaggt ttctttaaag
     2821 ccttcggtgg ctttgggttg aattaagcgc tcgacaaca
//
LOCUS       DAOQGS010000551         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21481_length_2848_cov_3.570354, whole genome shotgun sequence.
ACCESSION   DAOQGS010000551 DAOQGS010000000
VERSION     DAOQGS010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21481_length_2848_cov_3.570354"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /gene="hrpB"
                     /locus_tag="PKZ52_16080"
     CDS             complement(<1..1565)
                     /gene="hrpB"
                     /locus_tag="PKZ52_16080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNG61342.1"
                     /translation="MSAELPISPLLPELTAQLTHNHQVILQAPPGAGKTTQVPLHLLQ
                     APWLGTKKIIVLEPRRIAARSCAQYMASLLGQTVGKQVGYRVRQDSQVSTQTRIEVVT
                     GGVFLRMLQEDPSLEAVGLVIFDEFHERQLDSDLGLALSLYSQTLWREHDPLRLLVMS
                     ATLDTAALERLLPRAPVLTSEGRMFPVSLHYGKTLSPKDSIAEAVGAAVLEAMASYEG
                     NALVFLPGQREIQACQQWLSGRLDTSVRVFPLYASLPQAQQQQALAPDGAYRKIVLAT
                     NIAETSLTIEGITLVIDSGFCREAEFDPNTGLTRLNLKRISKASATQRAGRAGRLSAG
                     HCFRLWSQELVMNEHSPPEILKADLAPLALQLVAWGIEPGELAWIEPPPQAAYQQALE
                     LLAQLGAIKANVQGRWQLTASGEVISNMPVHPRLGHMLVASQAIGACEQACALASILT
                     ETQKGPGEADLSHLLEVLSTQPGSLPKPWRESVVRQAKQFAQLLNPPPATCQLSAGQI
                     GGFLLACAYPDRIA"
     gene            complement(1884..2546)
                     /locus_tag="PKZ52_16085"
     CDS             complement(1884..2546)
                     /locus_tag="PKZ52_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNG61343.1"
                     /translation="MFTRPLLASLVLGLSLTASSAFASVITNSKAAFTSGLTSYLVDD
                     YENPNYRHGDRIDASFLDAFSDTAMTHVLGETSYKTTGFANLNLMVGTTDHRYCAGCN
                     GSFLLDFSETSLTQGNGVSAIGFDFFKSSAYFATVSYGNGAVDNFQLSLGDGFWGITS
                     DQVIKSIHFGLLNGQATTSGYLEIDNLTIGQVAAKVPEPGNLALLGIGLLGLALSRRR
                     LK"
     gene            complement(2708..>2848)
                     /locus_tag="PKZ52_16090"
     CDS             complement(2708..>2848)
                     /locus_tag="PKZ52_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818489.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNG61344.1"
                     /translation="GQERLREAAKHGFKKAIVPVANAPKEPIAGLEVIGVRKLSEALE
                     QL"
BASE COUNT          631 a          743 c          835 g          639 t
ORIGIN      
        1 gctatgcggt cgggataggc gcaggccagc aaaaagccgc caatttgacc ggcggacaac
       61 tggcaggtgg caggcggtgg gttgagcagc tgggcaaatt gttttgcttg ccgtaccacg
      121 ctttcccgcc agggttttgg cagcgagccg ggctgggttg atagcacttc tagcagatga
      181 ctaaggtcgg cttcccccgg ccctttttgt gtctccgtca ggattgaggc cagggcgcag
      241 gcctgttcac aggcgccgat ggcttggctt gctaccagca tatggcctaa acgcgggtgt
      301 acgggcatgt tactgataac ctcgccgctg gccgttaact gccagcggcc ctgaacattg
      361 gccttgatgg cgcccagctg cgctaggagt tccagcgctt gctggtaggc cgcttgcggc
      421 ggaggctcaa tccaggcaag ctccccaggc tctatacccc aggccactag ctggagtgct
      481 aagggtgcca aatcagcctt gagtatctcc ggggggctgt gttcattcat caccagctct
      541 tgggaccaaa ggcgaaagca gtggccggca gacaagcggc cagcccgccc tgctctttgg
      601 gtggcggaag ccttggagat acgttttagg tttagccgcg tcaggcctgt attggggtcg
      661 aattccgcct ctcggcaaaa gccgctatca atcaccaggg tgatgccctc aatggtaagg
      721 ctggtttcgg caatattggt ggctaaaacg attttgcggt aggccccatc cggcgctagg
      781 gcttgctgtt gttgcgcttg agggaggctt gcgtagagtg gaaaaacgcg cacggaggta
      841 tccagccgcc cgctcaacca ctgttggcag gcctgaattt cccgctggcc aggcaaaaat
      901 accagcgcat tgccctcata actggccatt gcttctagca ccgcagcgcc cactgcttcg
      961 gcaatggagt ccttagggct gagggtttta ccgtaatgca ggctgacagg gaacatgcgg
     1021 ccttcactgg tgagtaccgg cgcgcggggg agtagccgct caagggccgc agtatccaaa
     1081 gtggcggaca tcaccaatag ccgcagaggg tcgtgttcac gccagagggt ttggctgtaa
     1141 agactcagtg ccagccccag gtcactatcc aattggcgtt cgtgaaattc atcgaagatc
     1201 accagcccga ctgcctcaag actcgggtct tcctgcagca tgcgcaggaa aacaccgccg
     1261 gtaaccacct ctatacgggt ttgagtgctg acttggctgt cctggcgaac cctatagccc
     1321 acctgcttgc ccaccgtttg gcccaagagg ctcgccatat actgagcgca gctgcgggcg
     1381 gcgatgcggc gcggttccag cacgatgatt tttttagtgc cgagccaggg ggcctgcaaa
     1441 agatgtaagg gtacttgggt ggtttttccc gctccgggtg gggcttgtaa aatcacctgg
     1501 tggttgtgcg tgagttgcgc ggtgagctca ggcagcagcg gcgagatggg gagctctgca
     1561 gacataggcg gcaagattaa taaaaggcgc ccgattatcc tcaaaactgc agatgaactc
     1621 tactaaggct gcccaataga tgaatctgca ggcttgcttt gaaatttaac tcaccttcta
     1681 agtgattact gctgtcgcca aatttttacc aaaaagcgtt tgattcagtg ctttaggcac
     1741 cctcggtagg atttcatttg ctgttgcagg attgcgcgta ctttgcccct ggcaagctgg
     1801 ggcgctcttg tggctggggt gaatatgggg caaaaaaaac gccggcccca agggaagccg
     1861 gcgccaaagg cctaaatgga aacttatttc agccggcggc ggctgagcgc taggcccagc
     1921 aggccaatac ccaagagggc gagattgcca ggctctggca ccttagcggc tacttggccg
     1981 atggtaaggt tgtctatctc caggtaacca ctggtggtgg cctggccgtt gagtaagcca
     2041 aaatgaatgc ttttgatcac ttgatcgctg gtgatgcccc agaagccatc gcccagtgac
     2101 agctggaagt tatccacggc gccattgcca taggaaacag ttgcaaagta ggccgatgac
     2161 ttgaaaaagt cgaagccgat ggccgaaacg ccattgcctt gagttaggct ggtttcagag
     2221 aaatccagta aaaaggaacc gttgcagccc gcgcagtagc ggtggtcggt ggtgcccacc
     2281 atgaggttaa ggttggcaaa accggtggtt ttgtaactgg tctcgccgag tacgtgagtc
     2341 atcgcggtat cggaaaaggc atctaagaag ctcgcatcga tgcggtcacc gtggcggtag
     2401 ttgggatttt catagtcatc tacaagatag ctagtaaggc cactggtaaa ggccgctttg
     2461 ctgttggtaa taaccgaagc aaaagcactg cttgcagtga gggacaatcc cagcactagg
     2521 ctggcgagaa gagggcgcgt gaacatcaat actactccgt tgaggggcta tgtcgggttc
     2581 cattcattag caattcaaca gcctaaatta aaaaactatt atttttcaat taattagtaa
     2641 atttaaaagc tctcgcttgt atgaaatatc gacgaaaaag ccgggtttga ctgtcgtcaa
     2701 atactaccta caactgctct aaggcttcac tcaacttgcg cacaccgatc acctcaaggc
     2761 ctgcaatggg ttctttaggg gcgttggcca ccggcactat agccttctta aaaccgtgct
     2821 tggccgcttc ccgcaggcgc tcctggcc
//
LOCUS       DAOQGS010000552         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21665_length_2832_cov_4.741808, whole genome shotgun sequence.
ACCESSION   DAOQGS010000552 DAOQGS010000000
VERSION     DAOQGS010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21665_length_2832_cov_4.741808"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /gene="pepN"
                     /locus_tag="PKZ52_16095"
     CDS             <1..1833
                     /gene="pepN"
                     /locus_tag="PKZ52_16095"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNG61345.1"
                     /translation="NIFNTSCVLAHPSTTTDAGFARVEGVVAHEYFHNWSGNRVTCRD
                     WFQLSLKEGFTVFRDEEFSADMLSRTVKRVESVNLLRTAQFAEDAGPMAHPVRPSSYM
                     EISNFYTLTVYEKGAEVVRMVHSLLGKELFRRGTDLYFSRHDGQAATIEQFIACMAEV
                     SGRDFSQFMHWYNQAGTPQVEFTGRYDEANKRFYLTAQQSCPSTPEARSEDKHPFVIP
                     IDMGLLGPQGAYPLICDGLKKSSPTRGVIELTASEQTFVFEQVPHAPVPSLLRGFSAP
                     VKWHYPYSKSDLLSLVLQDEDGFNRWDAMQQYAVRLINDINADFQNGRTPELDSGFIN
                     ACDELLQNEQLDPAMVALMLELPSEAYLAEQQNPMDIDGNHRAREFLRRAMAVHLLPH
                     WQQVYHRLVDNGPFSASAAAVARRSLKNLALSFIAAVDSAEHASLVAKQFNYATNMTD
                     SIAALKIAVNSDFPALQMHAGQMLAQFYERWQHEPLVVNLWLLTQAAAASNALNRVKT
                     LLAHTAYDGKNPNKIRALIGVFSQNLVHFHAVNGEGYEFLAQQVVNLDALNPQIAARL
                     IAPLTKWQRQDATRQALMVVQLRWISTHKLSNDLFEVVTKSLPV"
     gene            2236..2781
                     /locus_tag="PKZ52_16100"
     CDS             2236..2781
                     /locus_tag="PKZ52_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNG61346.1"
                     /translation="MPFKNTEKTYGLLAIAFHWLSALTVFGLFGLGLWMCSLDYYHPW
                     YKQGPDLHRSIGVLLVITTVLRLIWRSLNITPAGEPGLRWQLNLARITHGLLYLWFFL
                     MLISGYLITTAKGQALEVFNWFVIPAFITGIENLEDTAGVVHWLLACAFMSLVAVHAG
                     AAVYHHVIKRDGTLRRMLGRT"
BASE COUNT          684 a          666 c          718 g          764 t
ORIGIN      
        1 aatattttta atacttcctg tgtgctcgct catccatcta ccaccacaga cgccggattt
       61 gcgcgggtag aaggcgtagt ggcccatgag tattttcaca actggtcggg caatcgcgtt
      121 acctgccggg attggtttca gctgagttta aaagaggggt ttacagtttt tcgcgatgaa
      181 gaattttccg cggatatgtt aagccgcacg gtaaaacggg tggaatcggt aaaccttctg
      241 cgcacggcgc aatttgctga agacgcaggc cccatggccc acccggtgcg ccccagttct
      301 tatatggaaa tttccaattt ttacacccta acggtgtatg aaaagggcgc ggaagtggtg
      361 cgcatggtgc acagcctatt gggtaaagaa ttattccgcc gcggcacgga tctgtatttt
      421 tctcgccacg atgggcaggc cgctaccatc gaacaattta ttgcctgtat ggcggaagtc
      481 tccgggcggg attttagcca atttatgcat tggtataatc aggcgggcac cccccaagta
      541 gaatttaccg gccgttatga tgaagccaat aagcgctttt atttaaccgc ccaacaaagc
      601 tgccccagca ccccggaagc ccgcagcgaa gataaacacc cctttgtaat ccccatcgat
      661 atgggattat taggccccca aggcgcctac ccattaatct gcgacggcct caaaaaatcc
      721 agccccactc gcggagtaat agagttaacc gcgagtgagc aaacctttgt gtttgaacag
      781 gtgccgcacg cgcccgtgcc ctcattattg cgcggctttt ctgcgccggt taaatggcat
      841 tacccctata gtaaaagcga tttattaagc ctggtgttgc aggatgaaga cggctttaac
      901 cgttgggatg ccatgcaaca atatgcggtg cgcttaatta atgacattaa tgcagatttc
      961 caaaacggcc gcacgccaga gctggatagc ggctttatta atgcctgtga tgaattatta
     1021 caaaacgaac agctcgaccc tgccatggtg gccctgatgc tggaattgcc cagcgaggcc
     1081 tatttagccg agcaacaaaa ccccatggat atagacggca accaccgcgc gcgggaattt
     1141 ttgcgccgtg caatggctgt gcacttattg ccgcactggc agcaggtgta tcaccgctta
     1201 gtcgataacg ggccattttc cgcatccgca gctgcggttg ctcggcgcag cttaaaaaat
     1261 ctcgcgctta gttttattgc ggcggtggat agtgcagagc atgcctcttt agtcgccaag
     1321 caatttaatt atgcaaccaa tatgaccgat agcatcgctg ccttaaaaat agcggtgaat
     1381 agtgattttc ctgcacttca gatgcacgca gggcaaatgt tggcgcagtt ttatgagcgc
     1441 tggcaacacg agcctttggt ggtgaaccta tggttgttaa ctcaagcagc ggcggccagt
     1501 aatgcattaa atcgcgttaa aactctattg gctcacacgg cctacgacgg taaaaacccc
     1561 aataaaatac gcgcgctgat cggcgtattt agtcaaaacc tggttcactt tcatgctgta
     1621 aatggcgagg gctatgaatt tttagcgcag caggtggtga atttagatgc tctaaacccg
     1681 caaattgccg cccgcttaat tgccccctta accaaatggc agcgccaaga cgccactcgc
     1741 caggccctaa tggtggtgca attgcgctgg atttctactc ataaattatc gaacgattta
     1801 tttgaggtgg tgactaagag cctcccggtg tagccgctaa gttatgggtt tatgtttttt
     1861 gccgggcata aacaccttct cttttaaggg ggaaagctat atcaataagt tatgtgtttt
     1921 acagtaattt ttgcctgatt tttgcggtta cccataaacc ccaccccagc cctcccctta
     1981 aaaaggggag ggagcaaaat agcctagggc atcagtaaaa tgccgtggtt tgctgctttt
     2041 ttgctcctcc cccttttgaa gggggaggct gggtgggggt tttggggcag ggtgcaaatt
     2101 agtttgaaaa cagcccacat tttattttaa tgacaatgag ccgaatttta aggccaaggt
     2161 ggatcgccca cgaaatctta agaaatgagt ctaaataggt tcctaataaa taccctgtga
     2221 ctacctggag acaatatgcc atttaaaaac accgaaaaaa cctatggttt gctggcgatt
     2281 gcgtttcatt ggttgtcggc gctgacagta tttggcctgt ttggtttagg cctgtggatg
     2341 tgcagcttgg attattacca cccctggtat aaacagggcc cggatttaca ccgcagtatc
     2401 ggtgtgctgt tggtgataac aacggtgctg cgtttaatct ggcgcagtct gaatattacg
     2461 cctgctggtg agcctggcct gcgttggcaa ctcaatcttg cgcgtattac tcacggcctg
     2521 ctttatctgt ggttttttct tatgttgatt tcaggctatt taattactac ggctaagggc
     2581 caggcgttgg aggtatttaa ttggtttgtg atccctgcat ttattacagg gattgaaaat
     2641 cttgaagaca cggctggtgt tgtgcactgg ctgctagcct gtgcatttat gagcctggtc
     2701 gctgtgcatg ccggggcggc tgtgtatcat catgttatta aacgggacgg tacattgcgc
     2761 cgtatgttgg gaagaactta acagcttgtt gaaatactac cttgctagct ctggcggcgt
     2821 taaatttggc ga
//
LOCUS       DAOQGS010000553         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21668_length_2832_cov_4.032409, whole genome shotgun sequence.
ACCESSION   DAOQGS010000553 DAOQGS010000000
VERSION     DAOQGS010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21668_length_2832_cov_4.032409"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /gene="map"
                     /locus_tag="PKZ52_16105"
     CDS             complement(<1..687)
                     /gene="map"
                     /locus_tag="PKZ52_16105"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNG61347.1"
                     /translation="MAAVTIKTPDEIATMRIAGRMAAEVLEMIGDYVVPGVTTEQLNS
                     ICHDYITQVQNAVPAPLNYRGFPKSICTSVNQVVCHGIPSEKKVLKSGDIINIDVTVI
                     YNGYHGDTSKMYYVGEVAPHADRLCKITQECLYKGIELVKPGVQLGDIGHVIQQHAEK
                     HHYSVVREYCGHGIGRIFHEDPQILHYGKPGTGYEIKEGMTFTIEPMINAGKAATKLK
                     PDGWTVETKDG"
     gene            1068..2639
                     /locus_tag="PKZ52_16110"
     CDS             1068..2639
                     /locus_tag="PKZ52_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNG61348.1"
                     /translation="MEWLMDPTAWLGLFTLIILEIVLGIDNLIFIAVLADKLPPSQRE
                     RARLIGLSLALVMRLGLLASISWLVTLTEPFVELLGRGISWRDIILILGGLFLLFKAT
                     SELHERLEGRVHQAQKGPYRAKFWAVVTQIIVLDAVFSLDAVITAVGMVNHLSIMMIA
                     VVIAMGMMLLASRALTRFVEKHPTVVMLCLGFLLMIGLSLLVEGMGFHIPKGYLYCAI
                     GFSVLIEMFNQLARSNRTRNLIGERPLRERTAEAVLNMLGGKPDPEEVGDDVAAITQV
                     EEDKPVFAQDERVMIRGVLQLADRSVQSLMTPRPDLVWLDANTPHEQLLAKLANCTYT
                     RLLLCDGELDKMLGVIETRKVLTQMLLGERLDLKNLAEQPLVILEHTSALKVMTLLRQ
                     HPNPVVVVIDEYGGVEGMATLSDLLSAIVGDFATTQDPSAPLLTAGENGSWILDASMP
                     LEQLEALLAVELIDDEQEFYTLGGLILEQLQHIPEAGESFALAGFNFRVLTVDGRRIS
                     QVEVSRLPEEEDNSG"
BASE COUNT          574 a          718 c          820 g          720 t
ORIGIN      
        1 gccgtctttg gtttccaccg tccagccgtc cggttttagt ttcgtggccg ctttgcctgc
       61 gttaatcata ggctcgatgg taaaagtcat accttctttt atttcataac cggtgccggg
      121 tttgccgtaa tgcagaattt gtgggtcttc gtggaatatg cgccctatgc cgtggccgca
      181 gtattcgcgc accaccgagt agtggtgttt ctcggcgtgc tgttggatga catggccaat
      241 atcgcccagc tgcacgccgg gttttactag ctcaatgcct ttatacaggc attcttgggt
      301 gattttgcag agccggtcgg cgtggggcgc cacttccccc acgtagtaca ttttgctggt
      361 gtcaccgtgg tagccgttgt aaatcacggt tacgtcgata ttgataatat cgccgctttt
      421 taacactttt ttctcgctgg gaatgccgtg gcacaccacc tggttgaccg aggtgcaaat
      481 ggatttggga aacccccggt aatttaacgg cgcgggaacg gcgttttgca cttgggtgat
      541 gtaatcgtgg caaatactat tgagctgttc ggtggtgacg ccgggcacca catagtcgcc
      601 gatcatttcc aaaacctctg ccgccatgcg gcctgctatg cgcatagtgg cgatttcgtc
      661 aggggtttta atggttacgg cagccatagg gggtccagtt gaagtccagg ggaaaagcgc
      721 gccattctaa accaagtggt gggattgcac agccgggcag gggcagcctg cttcgcgttt
      781 tcagggcggc gcgggcggaa aaataacacc taaactttag gggttaaggt ggaaaagcgg
      841 ctgaggagcg gcgacttggc cggtgagttt tatcctgtag gttgccattt gccactgtgc
      901 ctcattagaa tcaaaccccc tttggcaacc aaccctgcct aaatgtgcac tcctagattg
      961 cctcccctac ccggggtgag gcgcagcaag accccatttg accgcatttt agttcccgct
     1021 acggcatgtc tacatcagcc tgatcccgca agggaggcct gctaaacgtg gaatggttaa
     1081 tggaccccac ggcctggtta ggcctgttca ccctgattat tctcgaaata gttctgggta
     1141 ttgataacct catcttcatc gccgttctgg ccgataaact cccccccagt cagcgcgagc
     1201 gcgcccgctt aattgggctg agcttagcgc tggtcatgcg cttaggcttg ctggcgagca
     1261 tttcctggtt agtaactctc accgaaccct ttgtggagct attgggccgc ggtatttcct
     1321 ggcgggatat tattcttatt ctcggcggct tatttttatt attcaaagct acctccgagc
     1381 tgcacgagcg cctggaaggg cgggtgcacc aggcgcaaaa aggcccctat cgggctaagt
     1441 tttgggcggt ggtgacgcag attattgttt tggatgcggt gtttagcctg gatgcggtga
     1501 ttacggcggt gggcatggtg aatcatctgt ccatcatgat gatcgccgtg gtgattgcca
     1561 tgggcatgat gctgttggcc agccgagccc tcacccgttt tgtggaaaaa caccccacgg
     1621 tggtgatgtt gtgtttgggc tttttattga tgattggcct gagcctattg gtggagggga
     1681 tggggtttca tatccccaag ggttatttgt actgcgctat tggcttttcg gtgcttatcg
     1741 agatgttcaa ccaattggcc cgctccaacc gcacccgcaa tttaattggc gagcggcccc
     1801 tgcgggagcg cacagccgaa gcggtgttaa acatgctcgg cggcaaaccg gacccggaag
     1861 aggtggggga cgatgtggcg gccattaccc aggtggaaga ggacaaaccg gtgttcgctc
     1921 aggacgagcg ggtgatgatt cgcggcgtgt tgcaactggc cgatcgctcg gtgcaatcct
     1981 tgatgacgcc gcgccccgat ttggtctggc ttgacgccaa taccccccac gaacagctgt
     2041 tggccaagct tgccaactgc acctataccc gcctgctgtt gtgtgatggc gagctggata
     2101 aaatgctcgg cgtgattgaa acccgcaaag tgctcacgca aatgctgctg ggtgagcgcc
     2161 tggatttaaa aaacctcgct gagcagccct tggtgattct tgagcacacc agcgcgctta
     2221 aggtgatgac cctgctgcgc caacacccca acccggttgt ggtggtgatc gacgaatacg
     2281 gtggggtgga aggcatggcc accttaagtg atctgttatc tgccatagtg ggggattttg
     2341 ccaccaccca agaccccagc gcgcccctgc tcaccgctgg cgaaaacggc agctggatac
     2401 ttgatgccag tatgcctttg gagcagctgg aagccttgtt ggcggttgaa ttaattgatg
     2461 atgagcagga gttttacacc ctcggcggcc tgatattaga gcagctgcaa cacatccctg
     2521 aggcggggga aagctttgcg ctggcgggct ttaatttccg tgtgttaacc gtagatgggc
     2581 ggcgcatctc gcaagtggaa gtgagccgat tacccgaaga agaggataat tcgggttaag
     2641 cctgcgcccc tggtctaaac gcaggtctat tttgctatca ccccaaagta gcgtgcaggg
     2701 ggcataaaac ttgtcgattt tatttactgt tttcagccta aaaaccgcaa tagggtttag
     2761 ctgtggtttt tagcttcaac ctaaaaaccg cagttgaact cgtagcgagg gtgcctaaga
     2821 cgctgaatat gg
//
LOCUS       DAOQGS010000554         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21689_length_2830_cov_3.754595, whole genome shotgun sequence.
ACCESSION   DAOQGS010000554 DAOQGS010000000
VERSION     DAOQGS010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_2830_cov_3.754595"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..708
                     /locus_tag="PKZ52_16115"
     CDS             283..708
                     /locus_tag="PKZ52_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61349.1"
                     /translation="MKSLKYWVLIILISGFVTACVKVDVCRWAITPSVDSVLYHHGYI
                     YKTPSIPKENQPPGSTIAQNRLVSEDKVVDLSVTHLTIPGQQMALVYCGGNGFRQGAL
                     AKPLFNAFDPPPNMLIFDYPGYGPVVKQPRWNLIRLRKY"
     gene            834..1208
                     /locus_tag="PKZ52_16120"
     CDS             834..1208
                     /locus_tag="PKZ52_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61350.1"
                     /translation="METTYNDLRSLVSSRAGLFKHFFRITIDSEAPDFSIDQALKNYS
                     YPIAILKSKNDPITPPEASEKLAKLLIRSGKSVIAVNMGAVDHKEILFQPCLANAILG
                     RIRPVVGGLSVSTQGCTRKSGY"
     gene            1434..>2830
                     /locus_tag="PKZ52_16125"
     CDS             1434..>2830
                     /locus_tag="PKZ52_16125"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNG61351.1"
                     /translation="MPGEALIQQEQQRQEVLRRERESTDAPFTVIPRGHLTPNAPPAD
                     SGPCFDIQTIAVEGVEAFSASRIAEMYSPFLNRCLAANDINALIRSLTNLYLDAGYIT
                     SRAYLPNQNLTQGHLRLVVVEGFVETVEIEGASRKRLSSAFPGIEGKTLNLRTIEQGI
                     DAIGRSGADAKVDFLPGSKLGATRLVVKVKNRPQRQFTLGVDNEGSKATGRTRVSLTQ
                     AWNNPIGFNDLAWLNVQTTALGRDDRRKATSVAASYSIPWGNWVETLSANQYQYESWV
                     SEAVVPFATSGDSSSISLDSRYLLWRNQTGLTHLGLRLKQQDTENRVSDVRLMTSSRQ
                     YSAASAYASYRHVGQRQEWQTELALHQGLSSDQAELGGATPDPHYQKWTLDASYLKRS
                     QWQQLPLEWRSQWQFQHSDDLLYASEQLNLGGQYTVRGFAEQSVQGTSGFFTRNSLST
                     IRPGRLDSAGFWQPS"
BASE COUNT          702 a          680 c          732 g          716 t
ORIGIN      
        1 cttgagaatc gatctcgtcg tgtgcattta ccttgggcca acgccaattg gcaccaaaaa
       61 atagagctaa ccgatcaaat attgtttgag caggggtatt ggctggtttt aaaaaaagct
      121 cgctgcccga cggtcgtcta tatcagagga tgctgacgct tccaacaaag aatcattatt
      181 cgtttattga acattgaaca ttgcgtatat gaaataggtg aatcggactt ttgtgcttaa
      241 aaatgaaaaa attagcgtgg ctagcgacgg gcgagaatta aattgaaatc gctaaagtat
      301 tgggtgctga tcattttaat tagcggtttt gtcaccgcct gcgtaaaagt agatgtgtgt
      361 cgttgggcaa tcactccgtc tgtcgattcg gttttatacc atcacgggta tatttataag
      421 actccgagta ttccaaagga aaaccaaccg cctggttcca ccattgcaca gaatcgactt
      481 gttagtgagg ataaagtagt cgacctatct gtcactcact taacgattcc cggtcagcag
      541 atggcgcttg tttattgtgg tggtaatgga tttcgtcagg gcgctttagc gaagcctcta
      601 tttaacgctt ttgaccctcc gcccaatatg ctgatttttg attacccagg atatggtccg
      661 gtggtgaagc aaccgcgctg gaatttgatc aggctacgca aatattaggc aaacacattt
      721 cacaatggca gcaaacacaa gggtttaaac gcattatatt ctgggggcgc tcttttggcg
      781 ggacaatatg cgcccgcttg gcgggacgtc atcctggtga gagctctctg gttttggaaa
      841 ctacgtacaa tgatttacgt tcactggtat cgtctagagc gggtttattt aagcattttt
      901 ttaggataac aatagattct gaagcgcctg atttctcgat agaccaagcg ttaaaaaatt
      961 attcctaccc cattgcaatt ttaaaatcta agaatgaccc gattactcca ccggaagcgt
     1021 cagagaaatt agcgaaactt ttaatccgta gcggcaagtc ggtcattgcc gtgaatatgg
     1081 gtgcagttga tcataaggag atactatttc agccttgctt agcgaacgct attttaggcc
     1141 gtatcagacc cgtggtcggc ggtttgagtg taagcactca aggctgtacg agaaaatcag
     1201 gttattaata gcgccagtat ccagccgcct cagcgactgc acgggccgtt ttacaggaaa
     1261 ttacttattt taaataaaat gttttcatat gatggcctta tttgctgctc cggtattaaa
     1321 atggcgcccc cgaattcaga gtctttttaa tgccaaggat tagtttactg gtcgttttaa
     1381 tcgggtttgg tttatccagt cgtgtagggg ccgctgagcc atctggcgct ggaatgcccg
     1441 gcgaagccct gattcagcaa gagcagcaac gccaggaggt attgcggcgt gaacgcgagt
     1501 caaccgacgc gccctttacc gttattcccc gtggacacct gacaccgaat gcccctccag
     1561 ccgacagcgg cccgtgtttt gatattcaaa cgattgcagt ggaaggcgtt gaggcgtttt
     1621 ctgccagtcg tattgccgag atgtacagcc cctttttaaa ccgttgtttg gccgccaatg
     1681 acatcaatgc gttaattcgt agccttacga atctgtacct tgacgcaggc tatatcacca
     1741 gccgcgccta cttacccaat caaaatctca cccaaggcca cttgcggttg gtggtggtag
     1801 agggctttgt agagacagtg gagattgaag gggccagccg taagcggctg agctcggcat
     1861 ttcccggtat tgagggtaag acgctgaatt tacgcaccat tgagcagggc atcgatgcta
     1921 tcggccgcag cggcgctgac gcgaaggtgg actttttgcc gggcagcaaa ctcggcgcta
     1981 cccgcctggt ggtgaaagtg aaaaaccgcc cccagcggca atttaccctg ggtgtggata
     2041 acgaaggctc caaagccacg ggtcgcaccc gtgtttcgct gacccaagcc tggaataacc
     2101 cgattggatt taacgacctg gcgtggttaa acgtccaaac cactgccttg ggccgtgatg
     2161 accgccgcaa ggctactagc gttgcggcca gttacagtat tccttggggc aattgggtag
     2221 agaccctcag tgctaaccaa taccaatatg aaagctgggt gagtgaagcg gtggtgcctt
     2281 ttgccacctc cggtgactct tccagtatca gtttggatag tcgctattta ctctggcgca
     2341 atcaaacggg attaacccac ctgggccttc ggcttaaaca gcaggacacg gaaaaccggg
     2401 tcagtgatgt gcgcttgatg accagctcac gccaatacag cgccgccagc gcctatgcca
     2461 gttaccggca tgtgggtcag cgccaggagt ggcaaaccga attagcgctg caccaggggc
     2521 ttagtagtga tcaagcagaa ctggggggcg ccactcccga tccccactat caaaaatgga
     2581 ccctggatgc ctcttacctt aaacgcagcc aatggcaaca actcccatta gaatggcgca
     2641 gccagtggca attccagcac agtgatgacc tgttatacgc cagcgaacag ttaaacctgg
     2701 gcgggcaata cacggtgcgc gggtttgccg aacagagtgt gcaggggacc agcggctttt
     2761 ttactcgtaa cagcctaagt accattcggc cagggcggtt ggacagtgcc ggtttttggc
     2821 agccgtctat
//
LOCUS       DAOQGS010000555         2819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_21828_length_2819_cov_4.294139, whole genome shotgun sequence.
ACCESSION   DAOQGS010000555 DAOQGS010000000
VERSION     DAOQGS010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21828_length_2819_cov_4.294139"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKZ52_16130"
     CDS             <1..565
                     /locus_tag="PKZ52_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252200.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNG61352.1"
                     /translation="FILPASQTRDKDALTEQGVGAVLDELSETFEYIVCASPAGIEHG
                     AFMALYFADEAVVVANPEVSSVRDSDRILGILQSKSRKAERGERVLEHLLITRYNPER
                     VAKGEMLGVKDVEEILATKLLGVIPECETVLKASNQGTPVIYDTESKAGKAYNDAVGR
                     LLGETIEHRFLTPEHKSLFQRLFRRSA"
     gene            562..810
                     /gene="minE"
                     /locus_tag="PKZ52_16135"
     CDS             562..810
                     /gene="minE"
                     /locus_tag="PKZ52_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNG61353.1"
                     /translation="MNFFEYFSRKKNTSASIAKERLQIIVAHERNKLKQPDYLPALQR
                     DIMEVLRKYVDIDMTQVEVQLENNGNCAVLELNVTLPE"
     gene            1174..2583
                     /locus_tag="PKZ52_16140"
     CDS             1174..2583
                     /locus_tag="PKZ52_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNG61354.1"
                     /translation="MSKPSPLEATESLKHKTDSWITDGAWISGVAGAVTVVLLAMLYN
                     ADKVLEKGLNIAGWQLTSERVDSLLAALCIITIVMVGIECVRIFKREGSQFIHKAPEL
                     GSGQTGKLFGEALTTYILNLILIGGVTLFYHSAGEYGFANKAPYYQPWFRLLEWILIF
                     YAIIGLPYAFVTRAYRHNPLLDKKDLSTLTGRVLAHILSFVPGLNHLRPEFDENDKKA
                     VLGLVVKMFFTPLMTVFFCDQFPHLVANVGYLLEGLPRTIAEGYYTHSKFNGDFFNIS
                     ISLIFSIDVALAWCGYMISSRWVDNQTASAEPSVLGWMVCISCYPPFQMLLGFYFSAP
                     GEHEVLKFTNQWMITIFTSMMVCSYIVYMSATLWFGVRFSNLTNRGIIRKGPFAYIRH
                     PAYASKNFAWWCVMFPAIIYNAQHTGMGLAGMQIVGLVLMTYTYYLRAITEERHLSAD
                     PVYLEYCKQVPYRFIPKVL"
BASE COUNT          718 a          697 c          647 g          757 t
ORIGIN      
        1 ttttatcctg cctgcctccc agacccgcga caaggacgcg ctcaccgagc agggggtggg
       61 agcggtactc gatgagttgt ccgagacctt cgagtacatc gtctgtgcct cccccgccgg
      121 tatcgaacac ggtgccttta tggcgctgta tttcgccgat gaagcggtag tggttgccaa
      181 cccagaagtc tcctccgtgc gcgactcaga ccgaatttta ggtattttgc aaagcaaatc
      241 ccgcaaggcc gaacgcggtg aacgagtact ggaacatcta ttaattaccc gctacaaccc
      301 cgagcgcgtc gctaagggcg aaatgttggg tgttaaagat gtagaagaaa ttcttgccac
      361 taaactgtta ggcgtcattc ctgagtgtga aaccgtatta aaagcctcca accagggtac
      421 accggtgatt tacgataccg agtctaaagc gggcaaagcc tataacgatg ctgttgggcg
      481 cttattgggc gaaactattg agcaccgctt tttaacgccc gaacataaaa gcctgtttca
      541 acgcctcttc aggagaagtg catgaatttt tttgaatact tctcacgcaa aaaaaatact
      601 tcagcctcca tcgccaaaga gcgtttgcag attattgtcg cccacgagcg caataaatta
      661 aaacaacccg attatttacc tgccctacag cgggacataa tggaagtgtt gcgcaagtat
      721 gtagacatcg acatgactca ggtggaagtg caattggaga acaacggtaa ttgtgcggtg
      781 ctggagttaa acgtcacact cccggaataa tgttcatcgc tgtgtttggc ggcctcaggc
      841 agctaaatgc agcgcacaac accccatgac tatttcttta gaaaggctgt atgggcctgt
      901 gcccgatggg ttttggcctt tgcgcgcaca agtgtgctaa gcggcctgcc accgccaatt
      961 aaagaagtga gatcactacc aactggcaac gctgctcgac ttaacaagtc cacaaaaatg
     1021 tacccacaaa ttcaccgccg ctgtgtacat ttgcaggcta taaacttaag ttacgcactt
     1081 aactaaccgc tttgcttgct cgctttaaga gctacggtgg ccaggttaaa ttgcgaggcg
     1141 ttaatccgaa caacaataat actgagactt gccatgtcaa aaccaagccc tcttgaggca
     1201 accgaatcac ttaaacacaa aacggactcc tggattaccg acggcgcctg gattagcggc
     1261 gtggcaggcg ccgtgactgt tgttctgctg gcgatgctat acaacgccga taaagtatta
     1321 gaaaaaggcc tgaatattgc aggttggcaa ctcaccagtg agcgcgttga cagcctattg
     1381 gcagcgctgt gtattatcac catcgttatg gtgggaattg agtgtgtacg catatttaaa
     1441 cgcgaaggca gccaatttat tcacaaggct cctgagctcg gcagtgggca aaccggcaaa
     1501 ttatttggcg aggccctgac cacctatatt ttaaatttaa ttttaattgg cggcgttaca
     1561 ctgttttacc attcagcggg tgaatatggc tttgccaata aagcccccta ctaccaaccc
     1621 tggtttcgtt tgctggaatg gatactaatt ttctacgcca tcataggcct gccctatgcg
     1681 tttgttacac gcgcctaccg ccacaatcct ttactggata aaaaagattt atccacgctg
     1741 acaggccgtg ttctcgccca tattttgtcg tttgtgcccg gcctaaatca tctgcggcca
     1801 gagttcgatg agaatgacaa aaaagcggtt ttaggcttgg tggttaaaat gtttttcacc
     1861 cccttaatga ccgtgttttt ctgcgatcaa tttccccatc tggtcgccaa tgttggttat
     1921 ttgttagaag gtttaccgcg caccattgcc gagggctatt acacccacag caagtttaat
     1981 ggcgactttt ttaacatttc catctcattg attttctcca tcgatgtagc ccttgcatgg
     2041 tgcggttata tgatttcatc gcgctgggta gataaccaaa ccgcctccgc agaacccagc
     2101 gtattaggct ggatggtgtg tatttcctgc tatccgccat ttcaaatgtt gttgggcttt
     2161 tatttttcag cccctggcga gcatgaagta ttgaaattca ccaaccagtg gatgattact
     2221 attttcacca gcatgatggt gtgcagctat atagtttata tgtcggcgac actttggttc
     2281 ggggtgcgct tttccaacct caccaatcgc ggcattattc gcaaagggcc ttttgcctat
     2341 attcgccacc cggcctatgc cagtaaaaat ttcgcctggt ggtgtgtgat gttcccagcc
     2401 attatttaca acgcccaaca cacaggtatg gggcttgccg gtatgcaaat agtcgggtta
     2461 gtgttgatga cctataccta ttatttgcgc gctatcactg aagagcggca tctttccgcc
     2521 gacccggttt atttagaata ctgcaaacag gtaccctacc ggtttatccc aaaagtttta
     2581 taacccactc tacccttgat actccacggc acatttgctg tggagtatca ggcggcatcc
     2641 aagtaagaaa cgtctgatta aataccgtca ccagcaagaa gccctgcgca aagttgtatg
     2701 gataggtttt taataaatgc gaattgatta gaaattgctt acgtgttttt cgacgggaac
     2761 ctttaaccta aaaaccgcag ttgaactcta ctacggccgc ctaatacttg aatctgcac
//
LOCUS       DAOQGS010000556         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22080_length_2802_cov_4.164179, whole genome shotgun sequence.
ACCESSION   DAOQGS010000556 DAOQGS010000000
VERSION     DAOQGS010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22080_length_2802_cov_4.164179"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..978
                     /locus_tag="PKZ52_16145"
     CDS             346..978
                     /locus_tag="PKZ52_16145"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HNG61355.1"
                     /translation="MINSVIDISHHNKVASFSKIKNAGIYGIIHKATQGTKYKDPTFT
                     ANRSNAVKAGIKVGAYHFGVAGDAIAQAEYLVATAGADSLLVLDFEGNPQGHDMSLDE
                     AEHFVDHINTLTGRYPGLYSGHTIKEALTKAGITNPSQTELAKCWLWIAQYSQAPLVP
                     KIWPRWTLWQYTDGAFGPEPHSLDGIGRCDRDQFNGSLEELDQFWCKNSG"
     gene            complement(1197..1982)
                     /locus_tag="PKZ52_16150"
     CDS             complement(1197..1982)
                     /locus_tag="PKZ52_16150"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNG61356.1"
                     /translation="MYRLLYPQLLSLAEQPAQVSKVILGLNWTLAEVSIEGAKSVGLS
                     FSPQDAPRILTWPGTLVGKNTAELAPWLKHWDPCEAAVGAAVTNAVINHRSVSLSQAQ
                     LLPAVPHIPPHLAVFAHFAPSVQGAKVVVIGSYPGLEASGLLGDFTCIERRVRPGDLP
                     DTAAEFILPEADWVFITASSIANKTLPRLLALAAQARVVLMGPSVPWLANWQDYGVDY
                     VAGVAIKDPAALAQTLMEGGGTRIFSQGVGYCVYATGRMREAF"
     gene            complement(1999..2373)
                     /locus_tag="PKZ52_16155"
     CDS             complement(1999..2373)
                     /locus_tag="PKZ52_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61357.1"
                     /translation="MEAQLQQRVQAEQDTIAAVEARLRAELARYGFLTQMAVLGQGMH
                     NQQLRIDDFDGNQSLYAEWQNAEGHCLGSLLIHGTGQVYAELDVLQDHPTDRKWYVES
                     VTCWGTAAALDCELGLLPALVD"
     gene            complement(2379..2702)
                     /locus_tag="PKZ52_16160"
     CDS             complement(2379..2702)
                     /locus_tag="PKZ52_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNG61358.1"
                     /translation="MPRPDIHVFICSQQRPAGHPRGSCGEKGAAALMPAFSQSLIKHN
                     LVNKVSLVATGCLGPCQVGASVLVFPGAHLYANVKEGDVETIIQEHLVGGAPVAAKLA
                     PAEIW"
BASE COUNT          633 a          819 c          789 g          561 t
ORIGIN      
        1 ttttgacaaa aaagcgtgca gattcaagta ttaggcggcc gtagtagagt tcaaccgcgg
       61 tttttaggat tatggcaaca ctgattaatt agctttttta aagcttatta agccagcggc
      121 atttaatacc ggggtaatag ccgcgtgtta tacaccttcc aacaatgaac actacatgcc
      181 aaaaatcctg gtgaatattc acgattgacc catttgttag caaagcctaa agtgaatttc
      241 cgcagcgaat tacaactggt ttttagcaaa taattcgtcg gctactcaac tgtctattag
      301 cgcacctcaa caatcatctg ccccacccca gcggaggcct tgcccatgat taacagcgtc
      361 attgatatat cccaccacaa taaagtcgca agtttcagca aaataaaaaa tgccggtatt
      421 tacggcatta ttcacaaggc cacccagggc acgaaataca aagaccctac ctttaccgcc
      481 aaccgaagca acgctgtgaa agcgggcatt aaagtggggg cctatcattt tggggtggcg
      541 ggggatgcca tcgcccaggc ggagtatttg gtggccaccg ctggcgccga ttcgctattg
      601 gtgctggatt ttgaaggcaa cccccagggc catgatatga gcctggatga ggctgagcat
      661 tttgttgacc acatcaatac cctgaccggc cgctaccccg gtctctactc aggccacacc
      721 atcaaagagg ccctgaccaa agcgggcatc accaaccctt cgcaaaccga gttagccaag
      781 tgctggctgt ggattgctca gtacagccag gcgccgctgg tgccaaaaat ctggccccgc
      841 tggaccctct ggcaatacac cgacggcgcc tttggccccg agccccacag cctcgacggc
      901 ataggccgct gcgaccgaga ccaatttaat ggcagcctgg aggagctgga tcagttttgg
      961 tgtaagaaca gtgggtaaat tgccgaagtg atactcacct tgtaagcggc acgccgcatt
     1021 tcaaaggcaa tcacttccat caagaaacgg ggtcgagagt agtcgtaggg gcgccccttg
     1081 tgggtgccca agcctgcgtg attcatgcgc ggtagccgga gcacacaggg caaccacgag
     1141 ggttgcccct acaggttcca ttaacttaac gacattacct ctagaggagg ggaattttaa
     1201 aatgcttccc gcatccgccc ggtggcatac acgcaataac ccaccccctg gctaaaaatg
     1261 cgggtgccgc cgccttccat cagggtttgc gccagggcgg cggggtcttt gatggcgacg
     1321 cccgccacat aatccacccc atagtcttgc cagttggcaa gccagggcac acttggcccc
     1381 attaatacca cgcgggcctg ggccgcaagg gccaacaggc gcggcagggt tttattggcg
     1441 atgctgctgg cggtgataaa cacccagtcg gcctcaggca gaataaattc cgccgccgta
     1501 tccggcagat cgccagggcg cacccggcgc tcgatacagg tgaaatcccc cagcaatcca
     1561 ctggcttcaa ggcctgggta actgccgatc actaccacct tagccccttg tacgctgggg
     1621 gcaaaatggg cgaagaccgc caaatggggc gggatatggg ggactgcggg cagaagctga
     1681 gcctggctta ggctcaccga gcggtggttg attaccgcat tggtcacggc ggcgcccacc
     1741 gccgcttcac aggggtccca atgtttgagc caaggggcga gttccgcagt atttttaccc
     1801 accaatgtgc ccggccaggt cagaatcctc ggggcgtcct gggggctaaa gctcaggccc
     1861 acacttttgg ccccctcgat gctgacttcc gccagggtcc aattcaggcc cagaatcact
     1921 ttgctcacct gggcgggctg ctcagcgaga ctaagtaact gggggtagag aagcctgtac
     1981 atgggcgcgt ttaacgggtt agtccacaag cgcgggcaat aggcccagct cgcagtctaa
     2041 ggcggcggca gtaccccaac aggtcaccga ctccacatac catttgcgat ccgtagggtg
     2101 atcctgcaat acatccagct ccgcataaac ctggccggtg ccgtggatca acaggctgcc
     2161 caggcagtgg ccttcggcgt tctgccactc agcgtagagg ctttggttgc cgtcgaagtc
     2221 atcgatgcgc aattgttggt tgtgcatgcc ctgccccaat acggccatct gggttaaaaa
     2281 tccgtagcgg gcaagctccg cccgcaggcg cgcctccacg gcggcgatgg tgtcctgctc
     2341 ggcctgcacc cgctgctgta attgggcttc catagggctc accagatttc cgccggagca
     2401 agtttggccg ccacaggggc gccgccaacc aagtgctctt gaataatggt ttccacatca
     2461 ccctctttga cgttggcgta gaggtgggca ccggggaaca ccagcacact ggctcccacc
     2521 tgacaggggc cgaggcagcc ggtagccacc agggagacct tattgaccag gttgtgcttg
     2581 attaaacttt gcgaaaaggc gggcatcagt gccgccgcgc ccttctcgcc gcagctgcca
     2641 cgggggtgac cagcggggcg ttgttgggaa caaatgaata cgtgaatatc gggcctaggc
     2701 attaccatct cctaagaaaa gtgcgcagcc cgccctgggc cgagcgtgcg cgcggcggcc
     2761 ctgcgggggc ggcaggttaa gctgcaccgg tgggcagcga ag
//
LOCUS       DAOQGS010000557         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22249_length_2789_cov_4.990124, whole genome shotgun sequence.
ACCESSION   DAOQGS010000557 DAOQGS010000000
VERSION     DAOQGS010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22249_length_2789_cov_4.990124"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKZ52_16165"
     CDS             complement(<1..182)
                     /locus_tag="PKZ52_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61359.1"
                     /translation="MSEQSKGIDGADAKRAAPKKRLGDVLIDKGLVSPDQVTIAITEQ
                     KKTGKALGNTLVDLGFV"
     gene            complement(179..2101)
                     /locus_tag="PKZ52_16170"
     CDS             complement(179..2101)
                     /locus_tag="PKZ52_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG61360.1"
                     /translation="MSLLLDALKKAALEKQRRSQDVDVSAAEPTLAAPESAVPDPAAN
                     TTRAAGVSLLANWLEPKQASVGFAEGLEATWVEPIKPAAAASHPRDLPAHTPGPVSLA
                     HAETATQAEAETQAETATQAEAATQAEAETAAEAETVACSAHLPPLDADSGSCRAEPQ
                     DATAECDFIDISDLDFAALVGFQAHVSEAPSAQVAAAHQVEIAAADTGAQAEPALAQP
                     LQALPLQTAASEPEPEPEPEPEPEPEPEPESEPSAQLAAAEPGPVAPAHYTPHSGKQA
                     LAELLHNSQRAAKRSRQRLLHLYGILVASAAGLVGFYYYALQVANPAVPAVALATAVA
                     PIAGGEPQAAEHTPSTASEARASEPAAAAPLNPAVADTLPKNAEPAGAPPQPAAAQLA
                     TPAKPAATAPEPVVAPSQEEAGAAQTLTPVDVPIRTGVTAVLAFHPAALDELAAAVER
                     GYTAYNAGDFVTAAAAYQSALQQDPYQRDALLGAAAVALQQGRTDEALNYYQRQLARA
                     PKDDFALAGLLAIGAQAGADPQLDAQLSRLIAAYPQAAHLHYLQGTLYAQRQQWGAAQ
                     LALLAAWQRDNGNADIACNLAIAFDHLQQPQEAVQFYRQALRLANGQAVNFAPAAIRT
                     RLSELEGAVVEERRRR"
     gene            complement(2108..>2789)
                     /locus_tag="PKZ52_16175"
     CDS             complement(2108..>2789)
                     /locus_tag="PKZ52_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61361.1"
                     /translation="NLLQATLKALETFGNVSIMSSPKVMALNNQTALLKVVDNLVYFT
                     VDVNIDTASTAVAGASRTVTYQTKVNTVPVGFVMSVTPYINEQGAVTLNVRPTISRVI
                     SQVRDPNPSLAEANVVSEIPVIQVREVESVLKVNSGDIAVIGGLMQDETNNAKTGIPV
                     LGKLPGIGAAFRYQDDKTVKTELVIFIRPMVVQHASLTSDLRAYKEYLPSRANPAPAG
                     QPPAAGRE"
BASE COUNT          474 a          852 c          864 g          599 t
ORIGIN      
        1 acaaagccca aatccaccag ggtattaccc aaggctttgc cggttttttt ctgttcggta
       61 atggcgatgg tgacctgatc ggggctgacc agccccttgt cgatcagcac atcgccgagg
      121 cgttttttcg gcgcggcgcg cttggcgtcg gcgccatcta tgcccttgct ctgttcactc
      181 atcgcctgcg ccgctcctca accacagccc cttccagttc gctcaaccga gtgcgaatcg
      241 cggcaggggc aaaattcacc gcctgcccgt tggccaggcg cagcgcctgg cgataaaact
      301 gcaccgcctc ctggggttgt tgcagatggt caaaggcaat ggcgaggttg caggcgatat
      361 ccgcattgcc attatccctc tgccatgccg ccaatagcgc caattgggcc gctccccact
      421 gctggcgctg ggcgtacagc gtgccctgta agtagtgcag atgggcggcc tggggatagg
      481 cggcaatcag gcggctcaac tgggcatcta actgcggatc ggcgcccgcc tgggcaccta
      541 tagccagcag cccggccagg gcaaaatcgt ctttaggcgc ccgggctagc tgccgttggt
      601 aatagttcaa agcctcatcc gtacgcccct gctgcagggc aacggcggcg gcccctaaga
      661 gcgcatcgcg ctggtagggg tcctgctgca aggcactttg gtaggccgcc gccgcagtta
      721 caaaatcccc cgcgttatag gcggtatagc cgcgctcaac cgctgccgcc agctcatcta
      781 gggcggctgg gtgaaaggcc agcacggcgg tgaccccagt gcgaatgggt acatctactg
      841 gggtgagggt ctgcgctgca ccagcttcct cctgggatgg ggcaaccacc ggctcaggtg
      901 cagttgctgc gggcttagcc ggtgttgcca actgcgctgc cgccggttgg gggggcgcgc
      961 ctgcaggctc ggcatttttc ggtagtgtgt ctgcaaccgc tggattcaat ggcgccgcag
     1021 ctgcaggctc tgaggctcgc gcctcggatg ctgtgctcgg ggtgtgctca gcagcctgcg
     1081 gctcgccccc tgcaatcggc gcaaccgcag tggcaagggc aacggcaggc actgcggggt
     1141 tcgccacttg cagcgcgtaa taataaaaac ccaccaggcc agcagcgctg gccacgagga
     1201 ttccatatag gtgcagcagg cgctgacggc tgcgcttggc tgcccgctga ctgttgtgta
     1261 acaactcggc aagggcctgt ttgccgctgt gtggtgtgta atgggcgggg gcaaccgggc
     1321 cgggttcagc agccgccaac tgggcactcg gctcggattc aggttcgggt tcgggttcgg
     1381 gttcgggttc gggttcgggt tcgggttcgg gttcagaagc ggcagtttga agcggtaacg
     1441 cctgaagggg ttgcgctagt gcgggctccg cttgggcacc tgtatcagcc gccgcaatct
     1501 ccacttgatg ggccgctgcg acctgtgcac tgggcgcctc gctcacatgg gcctgaaaac
     1561 ccactaaggc ggcgaagtct aagtcgctaa tatcgataaa atcgcactcg gcagtggcgt
     1621 cctgcggctc cgcacggcag cttccgctgt cggcgtcgag gggcggcagg tgggcggagc
     1681 aggcaaccgt ttccgcttct gccgctgttt ccgcttctgc ctgcgttgcc gcttctgcct
     1741 gcgttgccgt ttctgcctgc gtttccgctt ccgcctgcgt tgccgtttct gcgtgcgcca
     1801 aggagactgg cccaggtgtg tgtgcgggta ggtcgcgtgg gtgggatgcc gcagccgcag
     1861 gctttatggg ctcaacccag gtggcctcta gtccttcagc aaaaccgacg ctcgcctgtt
     1921 tcggttccaa ccagttcgcc aggaggctga cgccagcggc gcgtgtggtg ttggcggctg
     1981 gatccggcac agctgactcg ggtgccgcca aggttggttc agcggccgat acgtccacat
     2041 cttgggatct gcgctgtttc tccagagcag cctttttcag cgcatccaat agcaggctca
     2101 caggccatca ctcccggcca gcagctggcg gttgcccggc gggtgctggg tttgcgcggc
     2161 tgggcagata ctccttataa gcgcgcaaat cgctggttaa actcgcgtgc tgcaccacca
     2221 taggccgaat aaaaatcacc aattcggtct tcaccgtttt gtcatcctga tagcgaaacg
     2281 cagcgcctat gcccggcaat ttgcctaata ccggaatgcc ggttttggca ttgttggttt
     2341 catcctgcat caagccgcct ataaccgcta tatcaccgga attcaccttc agcacagatt
     2401 ccacctcccg cacctgaatc accggaattt cactgaccac attcgcttcg gctagagagg
     2461 gattggggtc gcgcacctgg ctgataaccc gcgaaatagt cggccgcaca tttaaggtca
     2521 ccgcgccctg ttcattgata tagggcgtca cgctcatcac aaaccccacc ggcacggtgt
     2581 tcactttggt ttggtaggtc acagtgcggg aagcccccgc caccgccgtg ctggcggtgt
     2641 cgatgttgac gtccacggtg aaatacacca ggttgtccac cacttttaac agcgccgttt
     2701 gattgttcag cgccatcacc ttggggctgg acataatgct gacgttgccg aaggtttcca
     2761 gtgctttaag cgtcgcctgc agtaaattg
//
LOCUS       DAOQGS010000558         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22283_length_2787_cov_3.848463, whole genome shotgun sequence.
ACCESSION   DAOQGS010000558 DAOQGS010000000
VERSION     DAOQGS010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22283_length_2787_cov_3.848463"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKZ52_16180"
     CDS             <1..320
                     /locus_tag="PKZ52_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61362.1"
                     /translation="ADTNIHSTYLPPLVDHLDLAASGIQAVVWCTGFNMRFEWIKLPV
                     LAEGYPQQKRGVTDVEGLYFLGLNWMHTWGSGRFYHVGRDAEYLSDVMAEVAAKTAQQ
                     PVM"
     gene            complement(463..1101)
                     /locus_tag="PKZ52_16185"
     CDS             complement(463..1101)
                     /locus_tag="PKZ52_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61363.1"
                     /translation="MGFGIKTKELNGSRIQNGLCSQCSAEAVYNAGSIKLLHFWGIPL
                     VPIYASAKVRCANCGSLVKLKQATAEAKANASSLITPLTLIKSAWGLAVAVVAIAGLL
                     ALGFISKEQEQKDLAAPMAGDIYIVKMASFISDYQQATTYPYGVLKISSVEGTKVHFL
                     ISKQAYGNMKSARKSLSSDGHNSDFYSDQSLDADLASLKTQYESGAIKDIRR"
     gene            complement(1434..2405)
                     /locus_tag="PKZ52_16190"
     CDS             complement(1434..2405)
                     /locus_tag="PKZ52_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61364.1"
                     /translation="MPKSITRLKSSPILGIMLGVFYGLTLRITFDWKLVHGFLEIISF
                     AFLINAPFVVGAIAVFFMPREKPPIIWQQLMVANIAMTFFLLAMFILVLEGLICIVLV
                     APVFFVAASLGGLAMGVVIKYFDRAGPTLNCLVLLPFLLGAYESQLPSSQTTGKVANS
                     VLVNAPAEQVFNQIATVKDIQPEELGFSFMHAIGLPRPLEANMSGTGVGAVRLSKWEQ
                     GVSFKERITYWQAPTRMYYEFDIPAGSVPREALDQHVELGGKYFTVTQGGYDIEAINA
                     HQSRLTLHTVYVNKSNLQLYGNTWSTIALADFHHSILQLIKRRAEKT"
BASE COUNT          740 a          644 c          655 g          748 t
ORIGIN      
        1 ccgccgatac caatatccac agcacttatt taccgccgct ggtggatcat ttagatttgg
       61 ccgcaagcgg cattcaagcg gtggtctggt gtaccggatt taatatgcgc tttgagtgga
      121 ttaaattgcc ggttttagcg gagggttatc cgcaacaaaa gcgcggcgtg accgatgtag
      181 agggcttgta ttttctcggt ttaaattgga tgcacacctg gggctcaggg cgcttttatc
      241 atgtggggcg ggatgcggag tatttaagtg atgtcatggc agaggttgcc gccaaaaccg
      301 ctcagcagcc tgttatgtaa atacggttac aagcctgctg aattctttgc tgaagcgagc
      361 tttaagagaa aaatcgggtt aattgtgcat ggattgcagg gcaattaaaa taaccccttg
      421 gctcgctata aagcagcgcc aagggttatt tacgccaggt ggttagcggc gaatatcttt
      481 aattgcaccg ctctcatact gggtcttaag gcttgccaaa tcggcatcta atgattgatc
      541 gctgtaaaaa tcgctattat ggccgtcaga ggagagcgat ttacgggcgc ttttcatatt
      601 gccgtacgcc tgtttactaa ttaaaaagtg aacttttgtc ccctccaccg agctgatttt
      661 cagcactcca tagggatagg tagtggcttg ctgataatcc gatataaacg aggccatttt
      721 gacgatgtaa atatccccag ccatgggggc ggccaaatct ttttgctctt gctctttact
      781 tataaatccc agcgccagca acccagcaat cgccaccacc gccaccgcaa gcccccaggc
      841 ggatttaata agtgtgaggg gtgtaattag cgacgatgca ttggcctttg cctcggctgt
      901 ggcctgttta agtttgacta gactgccgca attcgcgcag cgcactttgg cagatgcata
      961 aatcggcacc aagggtatgc cccaaaaatg caacagctta atactgccag cattataaac
     1021 cgcctcggca ctgcactggc tgcatagacc attttgaatg cggctaccat ttaattcttt
     1081 tgttttaata ccaaagccca tgaggtttct ccataagtaa taatagtgtt taagtgttaa
     1141 tgcgctttaa gacgttaagc cctttcggcg cgtgtaatgc acagatgagg ataattgcct
     1201 agagaacaca tgttgttgag taatgctcga tagggtgcgg cagattgcca gaatataaca
     1261 aggggcgcca tgctcggcgc ctcattttcg agctgaggaa cctctgaaaa ttacctcgct
     1321 cgctctggcg tcgttgaatt taccaaaaaa tgctcattta ttgcgtgtaa actccgcttt
     1381 tatgggtaaa ttcgcttagc cagcgcttcg ccgcctatat ttttcagagg ttcctaagtt
     1441 ttttccgcgc ggcgcttaat cagctgcaaa atagaatggt gaaaatccgc caatgctatc
     1501 gtgctccagg tattgccgta gagttgcaaa ttggatttgt tgacatacac cgtgtgcaat
     1561 gtaaggcgcg attggtgggc attgattgct tcaatgtcat aaccgccctg ggtaacggta
     1621 aaatatttgc cgcctagttc cacgtgttga tccagtgctt ctcgtggtac tgaccctgcc
     1681 ggaatatcaa attcataata cattcgagtg ggcgcctgcc agtaggtaat gcgctcttta
     1741 aaactaacgc cttgttccca ttttgataag cgcaccgctc ctaccccggt gccgctcata
     1801 tttgcttcca gagggcgcgg cagccctata gcatgcataa atgaaaaccc taattcctcc
     1861 ggctgtatat ccttaaccgt cgcaatttgg ttaaaaacct gttctgctgg agcattaact
     1921 aagacgctat ttgccacttt tccagtagtt tggctgctgg gtagttggct ttcataggcg
     1981 cccagcaaaa agggtaacag cacaaggcag ttaagtgtgg ggcctgctct atcgaaatat
     2041 ttaattacta cccccattgc tagtccgcca aggcttgcag caacgaaaaa tactggcgct
     2101 accagcacaa tacaaattaa gccctctaaa accagaataa acatcgctag gagaaaaaat
     2161 gtcatagcaa tgtttgcaac cataagttgt tgccagatga tgggcggttt ctccctcggc
     2221 ataaaaaaca ccgcaatggc ccccaccaca aaaggtgcat taattaaaaa agcaaaagag
     2281 atgatctcta aaaacccatg cactaatttc cagtcaaagg tgatacgtaa ggtgagcccg
     2341 taaaaaacgc ctagcataat gccgaggata gggctggatt ttagtcgtgt tatggatttg
     2401 ggcattggcg ggatcgcaga aaaactttgg ttaagcaagc tgctgaatca aagtgagtca
     2461 gtagtctgtg ggcagtatac ctagcagcca attctagctt ttatagacga tctggtgcaa
     2521 taaatagttc agatatgaag caataggtaa atagcggggc gaaatttata atttattctc
     2581 caatagtggc caaatgtaat catcgcccgc atctgcagga gggctgccag acaaattcgc
     2641 ctagccggtg cttcgccgcc tgcacttttt agcggctcct taaacattca aactaattat
     2701 gcctaccccg gagtaatttc caatacaaaa agtctaggga acctctgaaa aattacctta
     2761 ctcgccctgg cggcgttgaa tttggca
//
LOCUS       DAOQGS010000559         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22375_length_2780_cov_4.222752, whole genome shotgun sequence.
ACCESSION   DAOQGS010000559 DAOQGS010000000
VERSION     DAOQGS010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22375_length_2780_cov_4.222752"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(635..>2780)
                     /locus_tag="PKZ52_16195"
     CDS             complement(635..>2780)
                     /locus_tag="PKZ52_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61365.1"
                     /translation="AAVDMEVVIDRTTTVRKTLEDIEHTLLVALLLVVLVTYAFLRDL
                     RATLIPTIAIPVTLSATLVVIYFCGFSLNNLSLMAVIIATSFVVDDAIVVVENASHHI
                     QKGLTPVAAALQSLREQGFTLLTMNLALVAVFIPLIFMGGALGRLFREFSVTLTAAVF
                     ISLLVTLISTPMLCARLLKPARQNSPVASLGARFTGVYGKSVKACLRRRGFVMLAFIA
                     SIGLSLHLYSLIPKGFFPQQDTGRLNGQIVTEQSISFTEARKRIAQLMAIVGQDPAID
                     AYYEFTGGRGGGTSNTGSFYARLKPMDERRVSAQAVANRLRGKLAQVPGANLFVSPPQ
                     DLNFGARISSGAYQYSLLSDNVDDLRQWAPKVRAALSKLPELTDLNSDYQDRGLQLYL
                     QIDKDAAARLQVATNQIDAALNNAFGQRVVTTLYEPLNQYFVVVNLAQAFTQTPAALE
                     QIYVPNKQQQLIPLASFARWETRNLPLSINHQEQFAAVTLSFNLAANVSLDQAAAAIE
                     RTFNALQPPANLSGRFTGTAKIFQQSLASQPWLILCALLAVYILLGILYESTLHPLTI
                     LSSLPAAGLGALVALKITGTEFTLIALIGIILVIGIVLKNAIIMINCALQLEQQGTLT
                     SQQAIHQACLLRLRPISMTSIAAILGALPLALGAGDGAEIRQPLGVAIVGGLIVGQCL
                     TLYTTPVIYTYLQNLRKRFAQVSFNPGLIKNA"
BASE COUNT          656 a          677 c          832 g          615 t
ORIGIN      
        1 tattggccat ttgttgatcc ttgagaatat tttccggttt tttcggtaat tcatactcgg
       61 cgagagaccc caccccaacc ctcccgtgca caaatcactt ccctgtgatt tgccttaaag
      121 gcagcttcgc tgtgcgcatt gctcctgcaa tgtgtgaaaa ggggagggag caaaatgcct
      181 ggaactccag caaagtgctt tgtttgaagg gtttttgctc ctcccccttt tcaaggggga
      241 ggctgggtgg gggtttctgg cagtgaaaaa ttgtcatgaa aaacagatca aaatactcaa
      301 cgtttctcgt tcccacgctc aaaagaaaat acataggggg caggtaaatt tttatggagc
      361 tgatcaggta ggcggttcca tgctctgcgt gggaagaaac cccaccccag ccctcccctt
      421 aaaaagggga gggagcagaa taccgggaat ttcagaaaat gccttatttt gaagggttct
      481 tcgtagcaag gcggttatcg ctgcgcacag ctgtggcgtt ttagagccaa taaaacctgg
      541 atttcgcagt gcatacaggc tacaaatcaa attacatcag ctgaataaaa accccagcga
      601 cagttttaat actttgcgct ggggtttttg tgttttaggc gtttttaatc aggccggggt
      661 tgaagctcac ctgcgcaaaa cgtttacgca aattctgcaa ataggtgtaa atcaccgggg
      721 tggtgtagag ggttaggcac tggccgacga ttaatccacc gacaatcgca acccccaggg
      781 gctggcgaat ttcggcaccg tcgccagcac ctagggctaa gggcagggcg ccgagaattg
      841 ccgcgatact ggtcatggaa ataggccgta ggcgcagcag acaggcttgg tggatggcct
      901 gctggctggt taaagttccc tgctgctcca attgcaaggc gcaattgatc atgatgatgg
      961 cgtttttcaa cactatgccg atcaccagaa taatgccgat caaggcaatc agggtaaatt
     1021 ccgtaccggt aatttttaag gcaaccaggg ctcccaagcc agctgccgga aggctggaga
     1081 ggatggtcag tgggtggagg gtgctctcgt acaaaatccc cagcaggata tacaccgcca
     1141 gcagcgcgca gaggattaac cagggttggc tggcgaggga ttgctggaat atcttcgcgg
     1201 tgccggtaaa gcggccgctg aggttggctg gcggctgtag cgcgttaaag gtgcgctcaa
     1261 tggcagcggc agcctgatcc aaggagacat tagcggccaa attaaacgac agggtcaccg
     1321 cggcaaattg ctcctggtgg ttgatgctaa ggggcaggtt gcgcgtttcc caacgggcga
     1381 agctcgccag aggaattaac tgttgctgct tgttaggcac atagatttgc tcgagtgccg
     1441 caggcgtttg cgtgaaggct tgggccaggt tcaccaccac aaaatactgg ttaagtggct
     1501 cgtagagggt ggttacaacg cgctgcccaa aggcattgtt aagggccgcg tcaatctggt
     1561 tggtggccac ttgcaggcgc gcggcggcgt ctttatcaat ctgtaaatac agctgcaggc
     1621 cgcggtcttg atagtctgaa ttgaggtcgg tgagctctgg caatttgctc agagctgccc
     1681 gtaccttggg tgcccattgc cgcaggtcat ccacattgtc cgatagcagg ctgtattgat
     1741 aagcgccgga gctgatacgg gcgccgaaat tgaggtcctg gggcgggctg acaaataaat
     1801 tggcgccggg tacttgggca agtttgccgc gcaggcggtt ggccactgct tgggcgctta
     1861 cccggcgctc gtccatgggt ttgaggcgcg cgtaaaagga gccggtgtta gaagtgccgc
     1921 caccgcggcc accggtgaat tcgtagtagg cgtctatagc tgggtcttgg cccactatgg
     1981 ccatcaactg ggcgattcgc ttacgggctt cggtaaagga aatactctgc tcggtgacaa
     2041 tctggccgtt taagcggcca gtatcctgct gcgggaaaaa cccttttgga atcagactgt
     2101 ataggtgcag gctgaggcca attgaggcga taaacgccag catgacaaag cctcggcgcc
     2161 gcaggcaggc tttaacactc ttaccgtaca caccggtaaa tcgcgcccct agactggcga
     2221 cggggctgtt ctgcctggcg ggtttgagca ggcgggcaca gagcataggg gtgctgatga
     2281 gggtaaccaa tagggagata aacaccgcgg cggtgagggt gacggaaaac tcccgaaaca
     2341 gccgcccgag tgcgccaccc ataaaaatga gtggaatgaa tactgccacc agggctaggt
     2401 tcatggtcaa cagggtaaag ccctgctcgc gcagactctg cagggcggcg gctacgggtg
     2461 ttaggccctt ttgaatgtgg tggctggcgt tctccaccac cacaatggca tcgtccacta
     2521 caaagctggt ggcaataatt accgccatga gggataagtt attcaagcta aaaccgcaga
     2581 agtaaatcac taccaaggtg gccgacaagg tgactggtat ggcgatggtg ggaataaggg
     2641 tggcgcgcag gtcgcgcaaa aacgcgtagg tcaccaagac cactaacagc agcgcaacca
     2701 gtagtgtgtg ttcaatatct tcgagggtct tgcgcacggt agtggtacgg tcgatcacca
     2761 cttccatatc cactgcggcg
//
LOCUS       DAOQGS010000560         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22400_length_2778_cov_4.829600, whole genome shotgun sequence.
ACCESSION   DAOQGS010000560 DAOQGS010000000
VERSION     DAOQGS010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22400_length_2778_cov_4.829600"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PKZ52_16200"
     CDS             complement(<1..868)
                     /locus_tag="PKZ52_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61366.1"
                     /translation="MRMFTGPIERVEATAGGILWGRSLLWLAFLGPFFFITYGMCNLL
                     AKQRGITASIAFSWEHSIPVIPWTVIPYWSIDLLYGLSFMLCRGARQVDRHALRLLTA
                     QLISIAFFISLPLRFSFERPVVEGFFGPFFKVLTGMDEPYNQAPSLHISLLIIIWAQL
                     CKLPAPWRWGAHIWCGLIALSVLTTHQHHFFDLPSGAAVGLLCLWLWPERTACPLSLI
                     RQHQSVPGLAGLYACSALAACLLAAGFGGWAWWLLWPAIALALLAGMYGAWGAAGFQK
                     YAGQRSLAVEVLL"
     gene            complement(878..2632)
                     /locus_tag="PKZ52_16205"
                     /pseudo
     CDS             complement(878..2632)
                     /locus_tag="PKZ52_16205"
                     /inference="COORDINATES: protein motif:HMM:NF023569.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT          658 a          782 c          812 g          526 t
ORIGIN      
        1 gtagcaatac ctcaactgcc aggctgcgct ggcccgcgta cttctgaaag cctgccgctc
       61 cccaagcgcc atacatgccc gctagcaggg caagggcaat ggctggccac agcagccacc
      121 aggcccaacc gccaaaccct gcggcaagta ggcaggcagc caaggcactg caggcgtaaa
      181 gccctgctaa cccagggaca gactgatgtt ggcgaatgag acttaagggg caggcggtgc
      241 gctcaggcca tagccacagg cacaacaagc ccaccgcagc cccgctgggt aggtcaaaaa
      301 agtgatgctg atgggtggtg agtaccgaaa gggcaatcag cccacaccaa atatgcgcgc
      361 cccaacgcca gggggcgggc agtttgcaca actgggccca gatgataatc agcaggctga
      421 tgtgcaggga tggtgcctgg ttgtagggct cgtccatgcc ggtgagcact ttaaaaaacg
      481 gcccgaagaa gccctccacc accgggcgct caaagctaaa acgcaggggc aggctgataa
      541 aaaaggcgat ggaaatcagc tgtgctgtca acagccgcag ggcgtggcga tccacttggc
      601 gtgcgccccg gcagagcata aaggacaggc cgtagagcag gtcgatactc cagtagggaa
      661 ttacggtcca ggggataacg gggatgctgt gctcccagct gaaggctatg ctcgcggtaa
      721 tgccgcgctg cttcgccagc aggttgcaca tgccataggt gatgaaaaaa aacggcccca
      781 aaaacgccag ccacaacagg ctgcggcccc aaagtatgcc gccagcggtg gcttctaccc
      841 gttcaatggg gcccgtaaac atccgcatca atacccccta ggcgccgcgc ctgcgggcca
      901 gggacacggt aaaaatgccc cattcatcga tgcgctgggt gattttttca aagccagcag
      961 cggccactaa ttgatccatc tccacctggc tgcgcctgcg catcacccag gcctcgccgc
     1021 ctctgtggct ggtgagggcg cgggcgataa actccaactg tgggtgccag ggctgcccgg
     1081 tatagatcaa atacccgccg ggctccaccg catcggccat gcccgccagg gaggtgcgga
     1141 tcatctcgtt gctggggaat aactcataca ggccggagac tatgccgagt gtgggcttag
     1201 ggttaacccc ggcaatactg gctcggtcaa aggcatcgcc caaatcaaag cgggcgatag
     1261 cgcctaagcc cttagcttga atcagagcac tgccggaggt ttggttgagg gggctatagt
     1321 cccgcagcac tatggatgcg ggtaattcac cctgggtgag ggcctctaat acatagcggc
     1381 cgtggcctgc ggctatgtcc aaaatccgca cgggccgccc ggcctgtttc aactccccca
     1441 tggccagctg cagtaactct tctaagtgta ccttgcgttg gcggatgccc cgtcagccga
     1501 tggcatttaa atactgcgca tccaccaggc ggccgatggg gcctgcccct gccggggtgt
     1561 tgcggtagac ataatctaag gtgctgcccg aatcaaagcc ggtggtttgg cccagtttaa
     1621 taccatctga cagcagggcg cccaggcgca tattcagccg cgccacggcc caaaacagcc
     1681 ccttggggct catagggctt aggggcttgg caaggcgctg ggcttcatca aaagacgcgc
     1741 cggatttatc cgcatccagc aggcaaacct ctggtgccgg ggcgctaaat tgccgctcga
     1801 taaacgtgcg caccttcgcc actgcgccgg agcggtcgcg ctcgccgagg gtgtcgtgat
     1861 aaaacccctc caacacatgt tgctctttaa ccgtggagcc taggcgttga aaaaacgcct
     1921 gctggggttt tgtatgcacg acccaatcgg cgccggaaat taataattgc gtgggcaggg
     1981 taatcgcttg tgcgtccgcc actatgcgct cggcagtggc atacagatcc aacaacacat
     2041 tcacggaaat ggcgcgggca attaatgggt ccgtcgcgta ggatttttgc cgctccacat
     2101 cgtgggttaa atagctgggt ttaacgtagc tattcacaaa aaaattgccg atgcatttgt
     2161 gcaaaaggct taagcctgcg cgtgcaaacg gcacatacaa tttaatatca aaggcagggg
     2221 aggcaagaat ttggcagcga atattcggcg cgtaatcgtg cacccaagta gccaccaata
     2281 cagcccccac actttgtccc aatactgcta tatcccggta ggcaaagccg tgttggctgt
     2341 ggatatgatc aataaaggtg tctacatccc gcactgaagc ggcaaaactg ggggaataac
     2401 cgcgcgggcc aggggattta ccgtggcccc gtgcatccca ggcaaaaata tccatatccg
     2461 gcacattcag ttcatcgacc aaatggccaa tgcgggcgga gtgttcgtgg ccgcggtgaa
     2521 ataacaccag gcaaccgcgc ttaggttggg tggcgggcca atggcggtaa aacaagggtt
     2581 gctgatcgaa agtggtgaac tgagcttcag tacagggccg ttgcgtttgc ataaattctc
     2641 cttgcaaaag ggctgtagat gtaatgggaa aaatgcggca gtaattaccg gcgtaacctg
     2701 tgtatgccaa aaacgcggtt aggctttaac agcttgttga aatactacct tgctagctct
     2761 ggcggcgtta aatttggc
//
LOCUS       DAOQGS010000561         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22404_length_2778_cov_4.177011, whole genome shotgun sequence.
ACCESSION   DAOQGS010000561 DAOQGS010000000
VERSION     DAOQGS010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22404_length_2778_cov_4.177011"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            512..2620
                     /locus_tag="PKZ52_16210"
     CDS             512..2620
                     /locus_tag="PKZ52_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNG61367.1"
                     /translation="MLAHLFGKYKTLVVSIALFLLLDATVLMVNFYTSFQIANDAKTI
                     NLAGRQRMLSQRMVKSLYEVDQSLAEGQFPKSQLAELALSAQMFEETLLALDEGGMAK
                     GSSGEPQRLEAVGPGAGRDALLAAKELWRSYSPLVSAINKATSSEALVPSVAAAVAFA
                     RSNNANLLNYMNLLTQDIERVASAKANQLRTIQLIAIFLAVANFILILRHFLGHLRLS
                     DKALESAREETTNILETVNEGLFLLDKDLRIGTQYSAKLHEMFFHGQQDVNIGNCLFS
                     DFLRDRIKPDDLETAQRFISLLFRKNVKSNLIGDLNPLKRVEINIAEDEGGYTTKYMH
                     FDFRRVMTEDTIKQVLVTVTDITREVLLSKELDESKAENAQQIEMLTGILHANPHVLK
                     VFIQNAFDTLGRINELFKIQDKTTHALERKLKDVFVEVHNFKAEAASLSLATVVGLAH
                     EMESSIKQLLGQPMLTGTDLLAPVVLLEKLIAYMESIQALAEKVASLTLLAPNSVQKP
                     NNSTGRWHHLQEMAQLLAQRHDKKVAVHLTGFNELELAPEQSRLINDMCLQFIRNAVI
                     HGIEKPEIRRLNNKPEVGRIDLSLIQLQDGTLEVNVRDDGAGVDYEKIRNKALSLGLG
                     TDEELKHWSQKQLVGLIFKAGFSTADDLTDDAGRGVGMDVIVSRIQYMKGNIRLNSRK
                     GKACQFVVTLPKMQTERAAA"
BASE COUNT          790 a          549 c          682 g          757 t
ORIGIN      
        1 ctactgggcc agatttatag acaaaggctc aaactgatac ctacaccaca tcagactttt
       61 ttagggtcca gagaaattgt taggtttgct acagaacgaa agccgccatg tattaaaaca
      121 aattgagcca agcaaaaaga caattaaagt tacagtttgc cgcaattcgg tgcagggaaa
      181 atcgaccacg cacttattta attcatgcga aatttatgac gaatcagcgt tggttagatt
      241 ttcttgcgat taatttatgc gctaggtgtg cgctctggat gcttagttat atggcgtcaa
      301 gagaattgaa aatctgcata taaaaaataa atattgtgca cagtaacccc aaggcttgga
      361 tcaaatttat ctaaataaat ataaattcct tagctatacc taacattgag ttttttgctt
      421 gcttgcttga ttattgtgaa tggtgatcaa gacaaggttt taggtgccac cgtcagctga
      481 tggtcaccga ggatacgctg aggaattgcc tgtgttagca cacttgtttg gtaaatataa
      541 aacccttgtg gtttcgatcg ccttgttctt acttctggat gcaacggtgt tgatggtcaa
      601 tttctacacc tcgtttcaaa ttgccaatga tgctaaaacc ataaacctgg ctgggcgcca
      661 gcgaatgtta tcccagcgca tggttaaaag cctttatgag gtagatcaat ccttggctga
      721 ggggcagttc cccaaaagcc aattggcgga acttgctttg agcgcacaaa tgtttgaaga
      781 aaccctcctg gctttagatg agggtggcat ggcaaaaggt tcatctggcg agccccagcg
      841 attagaggcc gtgggcccag gggcagggcg agatgcgttg ttggccgcta aagagctgtg
      901 gcgcagttac agccctttgg taagcgccat taataaagct accagttcag aagccctagt
      961 gcccagtgtg gccgctgcag tggcatttgc ccgtagcaat aatgcgaatt tactaaatta
     1021 catgaacctg ttgacccaag acattgaacg tgtggcgagt gcaaaagcga atcagctgcg
     1081 cacaattcaa ttaattgcaa tttttttagc agtagctaac tttattctga ttttgcgcca
     1141 ttttcttggt cacttgcgcc ttagcgataa agcccttgag tcagccagag aggagactac
     1201 caatattcta gaaacagtga atgaaggact ttttttgctg gataaagacc tgcgtatcgg
     1261 cacgcagtat tcggccaagt tacatgagat gttttttcat ggccaacagg atgtgaatat
     1321 aggtaactgt ttgttcagcg atttcttacg ggatcgaatt aagcctgatg atcttgaaac
     1381 cgctcagcgg tttatcagcc tgctgtttcg caaaaatgtt aagtctaacc tcattggaga
     1441 tttaaacccg cttaagcgtg tcgaaattaa cattgcagag gatgaagggg gttatacaac
     1501 aaaatatatg cattttgatt ttcgtagagt gatgactgaa gacactataa agcaggtgct
     1561 cgttacggtg accgatatca ctcgggaagt gcttttaagt aaagagttgg acgagtctaa
     1621 ggcagagaat gcccagcaaa ttgagatgtt aacgggcatc ctgcatgcca atccgcatgt
     1681 gcttaaagtc tttattcaga atgcttttga taccttaggc agaattaatg aattattcaa
     1741 aatacaggat aaaactaccc acgccctaga gcgtaaactt aaggatgtat ttgttgaggt
     1801 gcataatttc aaagctgaag cggcaagttt aagcctggct acggtggttg gccttgctca
     1861 cgagatggag tccagcatta aacaattatt ggggcagcca atgcttacgg gcacagattt
     1921 attggcaccg gtagtactgt tagaaaaatt aattgcctat atggaaagca ttcaggcgct
     1981 ggcagagaag gtagcaagcc ttacattact tgcaccaaac tcagtgcaaa agccgaataa
     2041 ttcaaccggc cgttggcacc atttgcagga gatggctcag ttgttagccc agcgacacga
     2101 taaaaaggtg gccgtgcatt tgacgggttt taatgagtta gaattggcgc ccgaacaatc
     2161 ccgcttaatt aatgacatgt gcctgcagtt tattcgcaat gcggttattc atggtattga
     2221 aaagccagaa attcgtcgcc taaataataa gccggaagta gggcgcatcg atttaagttt
     2281 aattcaactg caggatggca cattagaggt taatgtgcgg gatgatggcg ccggtgtgga
     2341 ctacgagaaa attcggaaca aagcactaag ccttgggcta ggcacagacg aagagctcaa
     2401 acattggagc caaaaacaat tggtggggtt gatttttaaa gcggggtttt ccaccgctga
     2461 tgacttaaca gatgatgccg gccggggtgt gggtatggat gtaattgtct cgcgtattca
     2521 atatatgaag ggcaatattc ggcttaacag ccgcaaaggc aaggcctgcc aatttgtggt
     2581 gactctccca aagatgcaaa ctgagcgagc ggcagcctag gttatgaata gtctattgaa
     2641 gcttaataaa acctttacca tgatggtggt ggatgattct aatgtaatcc gccgcaagat
     2701 cgagcgcggt gccgatggta cctatttttc agtagttgct actgccaatt ctgggcttga
     2761 ggcggtgctt gcttaccg
//
LOCUS       DAOQGS010000562         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22728_length_2753_cov_4.550408, whole genome shotgun sequence.
ACCESSION   DAOQGS010000562 DAOQGS010000000
VERSION     DAOQGS010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22728_length_2753_cov_4.550408"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKZ52_16215"
     CDS             complement(<1..655)
                     /locus_tag="PKZ52_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNG61368.1"
                     /translation="MKFSTSPRRPFLKKLALSIAALALAGNVSHASAAVGKPEKWELK
                     FGFIKLTDMVPLAVAYEKGYFEDEGLFVTLEPQANWKVLFDRVVSGELDGAHMLAPMP
                     LGSATGVITKAEIISPFTLSYNGAGITVSNAVWDEMKKNVPMEGGKPKHPISAEALKP
                     VLESYKKAGKQLNLGMTFPVGTHNYLLRYWLAAGGVNPGLYSASDASGTAGAQAMLSV
                     "
     gene            complement(1329..2042)
                     /locus_tag="PKZ52_16220"
     CDS             complement(1329..2042)
                     /locus_tag="PKZ52_16220"
                     /inference="COORDINATES: protein motif:HMM:NF021141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HNG61369.1"
                     /translation="MPSSLSRPLFSSPRLFMALCYGLLACCLVSLNGCVTLPKWDASA
                     ARQWRHAIYYEYESWHSSIIIPAPLVAKHSERFGHLAAKREYLGFGYGDINYFTGRDT
                     SLRSGAKALAFSDGPALQVLDYVQDPFAKMPPDRYVKLLVNETQLKNVLRYMDNSVAL
                     DGNGSPRSIKNDEPNTGFFFVAQGRYSMFSNCNTWAGDALNAAGLPISKTWGLTAGGV
                     YRQAKELARAQERLLAQPY"
BASE COUNT          648 a          646 c          764 g          695 t
ORIGIN      
        1 caacagataa cattgcctgg gcaccagctg tgccgctggc gtccgatgca ctgtacaaac
       61 cggggtttac accaccggct gctaaccaat aacgcaataa ataattgtga gtgcccaccg
      121 gaaatgtcat gcccaagttc agttgtttac cggctttttt ataggactct aaaacgggtt
      181 ttaatgcttc ggcactgatg gggtgtttgg gtttgccacc ttccataggt acgttttttt
      241 tcatttcatc ccaaacagca ttggaaacgg tgatgcctgc accgttatat gaaagtgtaa
      301 atggagaaat gatttctgct ttggtaataa cgccagtggc tgaacctagg ggcataggtg
      361 ccaacatatg ggcaccatct aattcaccgg ataccacgcg gtcgaataat actttccagt
      421 tggcttgagg ctctaaggtg acaaacaagc cttcgtcttc aaagtagcct ttttcatagg
      481 ctacggccaa gggcaccatg tcggttaact taataaagcc gaattttaat tcccactttt
      541 caggtttacc tacggcagca ctggcgtgcg acacattgcc tgccagtgct aaggcagcaa
      601 tggaaagtgc taattttttt aaaaaagggc ggcggggcga cgtgctgaac ttcatggtaa
      661 atcctcgtgg cgataattaa atgcgggtgt gtttaggctg ccaacgcacc cgagttaatc
      721 caggtgttag caagcaaacc tagataaatt cggtgactgg ggaagctaat tcaatagagg
      781 tgccactgaa atatttttct ttaatatcag tgagctataa attaattgcg ccgttgaggc
      841 cgcctaatgg ggccgcttgc ggccagtctt aggtgcacgc taaaaaatat ttgttcgttt
      901 taagggcgat gtgggtgctg tgaattattt tttaacgcat cagatcgttg ctggttactg
      961 tatttgattt tttttggtgc gttattgagg cgaattttta ctattgagga gcactttttt
     1021 taaggcaaac cgcttttttt cgatgcgcag gctaaattat tgccctatga gtgagcgaat
     1081 aaggtagctg ctgggcaagc tctatttagg gccggtgggg agcaatgtta ttaatgaagg
     1141 attgcctaac gcgcacatag gcgtatgggc gccctctgtg gatgcccgcg ctcccgtagg
     1201 tactgatcta tggccagggc aaccacaagg cttgcctcga aacatcctat tgcgtgagta
     1261 ggtgcagctg aggagttttt atcaaggacc ggtgaaaggt gcagccgggt ttaactgcac
     1321 tgtttaggtt aataaggctg ggccaacagg cgctcttggg cgcgggcgag ttctttggcc
     1381 tgccggtaca caccgcctgc cgtgagcccc caggttttgc tgatgggcag ccccgccgca
     1441 tttaaggcgt cccccgccca ggtattgcag ttagagaaca tgctgtagcg gccctgggca
     1501 acaaaaaaga agccggtatt gggctcgtca tttttgatgc tgcgggggct accattgcca
     1561 tccagggcaa ctgagttgtc catataacgc agcacatttt ttaactgggt ttcgttgacg
     1621 agcagtttta cgtagcgatc cggcggcatt ttggcgaagg gatcttgcac atagtccagc
     1681 acctgcagcg cggggccgtc tgaaaaggcg agggctttag cgccggagcg caggctagtg
     1741 tcgcggccgg taaagtagtt gatgtcccca tacccaaagc ctaagtattc gcgctttgcg
     1801 gccaaatggc caaagcgttc gctgtgtttg gccactagcg gggcggggat aataatgctg
     1861 ctgtgccagc tttcgtattc ataataaata gcgtggcgcc attggcgggc ggcgctggca
     1921 tcccatttgg gcagggtgac gcagccgttt aggctcacca aacaacaggc gagcagccca
     1981 taacacaagg ccataaaaag gcgcgggctg gaaaataagg ggcgggataa cgaactgggc
     2041 ataggcgaaa aaggcgccat tatagccagg ccggggcggg gcacaaactt ggcgaagcgg
     2101 gcgacttaag aagagctgtt ggcagcaatg ccatgcagtg aaggattttt ggaggttgca
     2161 ctgcctaaaa ttccccagct gtcgagggca atgtcattaa gttagtgttt tcgcactatt
     2221 gttccagaga attttctctc atccagaaac cccaccctcc ccttgaaaag gggagggagc
     2281 agagcagcct gcagccaata ttctttgtgt tgaaggttta ctgcccctcc ccctttgaag
     2341 ggggaggctg ggagggggtt tctagcggta aaaataggct caaattctta atttaatgat
     2401 attgctgtgg agggggggca ggcctgcctg ccgggtggtc gcgatctcag tgttattttt
     2461 cgtgggacga gatagtgttc tagctatggt ttttgcctat tggagcgcag cgggctgcga
     2521 accaaaaaca acgcggtgac ctaccacctc acccctatgg cgcacccctt tcgcaggagg
     2581 gcaattttgg ccctgcccag ggcgaaaaaa ggccagcaac tttctaccca ggttggcaaa
     2641 acgctccttg tattgttagc taaaagcccc tagctgcccg cgatggcgct attgaccagc
     2701 gtctgcgcct cgccaatcaa ctggctcagg tgctcgctgc cgacaaaact ctc
//
LOCUS       DAOQGS010000563         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_22998_length_2735_cov_4.445522, whole genome shotgun sequence.
ACCESSION   DAOQGS010000563 DAOQGS010000000
VERSION     DAOQGS010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22998_length_2735_cov_4.445522"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKZ52_16225"
     CDS             <1..329
                     /locus_tag="PKZ52_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61370.1"
                     /translation="RCKFATNPAITSYHFYRKLDAAAGDPLVGMAADIDYTGELILTR
                     HGTANSGRIEVVFEGKVDSFPAYECYANFGGVTKLLFQLRPPAGNTVLNLLGAATTPV
                     SGRASF"
     gene            719..1801
                     /locus_tag="PKZ52_16230"
     CDS             719..1801
                     /locus_tag="PKZ52_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61371.1"
                     /translation="MSTSLSHINPSDYIYFSPKTANGCAGKPVKIHKDLAVAFITAVN
                     PKSQEGQDAVLKIEQLTALAQDTQAQGTDNAAVKTKEVGCLTVCYTITQAQGDRTPSV
                     YITNFRQRFASDQKTSGLYYVEHQKYTADIEKIEALTHRTLFINGLANNINAACENSF
                     ARLAAREYNLFYTSGNDFKLMGAWSQAESTQVSAKLAKVLNSYAGADVTVYVEGYGAH
                     VLHSALPQVRELSKFSFVLENPVTNIASLIQAIDAKKGKYSHADIVKIYPGHTHLPEF
                     NASKVALAYSRLSLCEKVIKSGSGDNYALVLVQLYQDTNVPTNKLEEGKRYLDPRKNH
                     AYTTDKNISFNTNTFIEGVNKMAAWR"
     gene            1798..2544
                     /locus_tag="PKZ52_16235"
     CDS             1798..2544
                     /locus_tag="PKZ52_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61372.1"
                     /translation="MKKYPNGASGPNLEQCDIITFDLQDAQLQFCLPGQSTNIRSVLA
                     SQESLSNWQSTIDFKLLAKNEWGYTATDEAGEWGVASVFMDMKLINLDLDEFESWLGV
                     TPYCLLKKEELSRLAIEYFTKTALDRSRKFNPDISAEEALKRYRTFTSPEDLLISKQD
                     ILPWYTNKLVRIDGNGSSLIQTFIPISERAVIFFLFDITEQTLDRPFYFTNEECQAFG
                     DMLRDEFLSYVKITYSLEIQEKIKLYAQNP"
BASE COUNT          836 a          703 c          545 g          651 t
ORIGIN      
        1 cgcgctgtaa attcgcaact aaccccgcca tcacctccta ccatttttac cgcaaactcg
       61 atgccgcagc aggagaccca ttggtgggca tggccgccga tattgactat accggcgagt
      121 taattctcac ccgccacggc acagcaaact ccggccgtat tgaagttgtt tttgaaggca
      181 aggtagacag tttccccgcc tatgagtgtt atgcaaattt tggcggcgtt acaaaattgc
      241 tcttccagct gcggccccct gcaggcaata cagtgcttaa tttactgggc gccgccacaa
      301 cccccgtcag tgggcgcgcc agtttttaat ttatttatcc attaggttgg gcagcaatgc
      361 ccaactccta tcaatcaaag aaaataatcc ttcaccccaa tgtacgtttt gcaaaaatat
      421 atttattgct gattgagacg gaaatacata tctaatcgaa acataaaaac aacaaacctg
      481 cgaaaatttg tgctcaaaat taggcatata aaaattcggc ccttgggcca ataatcgcct
      541 tataaccgat ttagcagacc catccagcaa cttcaacaat cgggctcacc aattcttgcc
      601 taatcttgcc taagccagat gtggcagata ctattagttg gtttagcgtc agcccagcca
      661 ctggcctgcc gctaccaacc aacttgcccc attaaatgct gtaaaaaagg aacccaacat
      721 gagtacctcg ctatctcaca tcaaccccag tgattacata tatttctccc ccaaaaccgc
      781 caacggctgc gcaggcaaac ccgtcaaaat ccacaaagac ctagccgttg cctttatcac
      841 tgccgtaaac cccaaatctc aggaggggca agatgcggtg cttaaaatag agcaactcac
      901 cgccctagcc caagataccc aagctcaagg gacggataat gctgcagtaa aaaccaagga
      961 ggtgggctgc cttacggtgt gttacaccat cacccaagcc cagggggatc gaacgccttc
     1021 tgtctatatc acgaatttta ggcagcgttt tgcgtccgac caaaaaacct ctgggcttta
     1081 ctatgttgag caccagaaat acacagccga tattgagaaa atagaggcat taacgcacag
     1141 aacgcttttt atcaatggat tggcaaacaa cattaacgct gcctgcgaaa attcatttgc
     1201 acgtctagct gccagggaat acaacctatt ctatacatct ggcaatgatt ttaagttaat
     1261 gggcgcttgg tctcaggcgg aatccacaca agtatccgca aaattggcga aagtgctcaa
     1321 cagctacgcg ggtgcggatg taactgttta cgtcgagggt tacggcgcac acgtgttgca
     1381 ttcggccttg ccccaagtta gagaattaag taaattttca tttgttcttg aaaatccggt
     1441 aacgaatata gccagcctaa ttcaggctat cgacgccaaa aaaggcaaat attcacacgc
     1501 agatattgtc aagatttatc ctggccatac acatttaccg gaatttaacg catctaaggt
     1561 tgctctagcc tacagccgac tttcgctgtg tgaaaaggtt ataaaatctg gctccgggga
     1621 caactatgca cttgttctgg tgcagctata ccaagacacg aacgtgccca caaacaaact
     1681 ggaagaggga aagagatatc tcgacccaag gaagaatcac gcttacacaa cagataaaaa
     1741 tatctcgttc aacaccaaca catttatcga aggcgtaaac aaaatggcgg cttggcgatg
     1801 aaaaaatacc caaatggtgc ctccggccca aatctagaac aatgcgacat catcactttt
     1861 gatctgcagg atgctcaatt acagttttgt ttgcccggac aatcaaccaa tattcgcagc
     1921 gttctcgcaa gccaagaatc gctcagcaat tggcaatcca caatcgactt caagctactc
     1981 gccaagaatg agtggggata cacagctacg gatgaagccg gtgaatgggg tgttgcctct
     2041 gtttttatgg atatgaagct gattaaccta gacctggatg agtttgaatc ctggctaggt
     2101 gtaacccctt actgtttact aaaaaaagag gagttaagcc gcctcgcaat tgagtatttt
     2161 actaaaacag ccttagatag atcgcgaaaa tttaaccccg acattagcgc ggaggaagca
     2221 ttaaaacgct acagaacatt tacctcacca gaggatttac taatatccaa acaagatatc
     2281 ctgccgtggt ataccaataa gctggtgcgc attgacggca atgggtcctc actaatccaa
     2341 acatttatcc ccatttccga gcgagctgtt atatttttcc tattcgacat aactgagcaa
     2401 acgcttgaca gaccattcta cttcacaaac gaggagtgcc aagcatttgg agatatgcta
     2461 cgcgacgaat ttctaagcta cgttaaaatc acctacagcc tagagatcca agaaaaaatt
     2521 aaactatacg cacaaaaccc ataacccaaa gctgggcgaa agcgcccagc attatcagct
     2581 caagccgaca aattgcgcca aagccgtgtc atactcatat ctacaaaacg attgaaatca
     2641 aaccttaaaa tgaaccgcgc cccaggcaca tcaatgaaat ttttattccc catttacctg
     2701 gccatcacct gcaatttagc ctctgcggac tcctg
//
LOCUS       DAOQGS010000564         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23044_length_2732_cov_3.662309, whole genome shotgun sequence.
ACCESSION   DAOQGS010000564 DAOQGS010000000
VERSION     DAOQGS010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23044_length_2732_cov_3.662309"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKZ52_16240"
     CDS             <1..312
                     /locus_tag="PKZ52_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61373.1"
                     /translation="GCDAAGAGIVVGDPNTLSNYIDGDVVVVPPAANANGNYAQVTIP
                     LDWANLGANGGTADMVVTIFRCSSGVVRRCASTVAADYKFFPNSCRNTVAQITAVPAG
                     C"
     gene            complement(555..1010)
                     /locus_tag="PKZ52_16245"
     CDS             complement(555..1010)
                     /locus_tag="PKZ52_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNG61374.1"
                     /translation="MPFINKFKTFTDSWWGFLITLGIVYGGVKGLQNYWGGQDFADTG
                     LPKITLAEGLKRAQTENKFIVAELSATWCGTCRTMDRTVFSDPEVKRFLTEKVIYVRI
                     DEKDAEAIFFTQTYNAQGYPTIVLLNTQGVLQRHLPLTYTPNEFLQTLR"
     gene            complement(994..2052)
                     /locus_tag="PKZ52_16250"
     CDS             complement(994..2052)
                     /locus_tag="PKZ52_16250"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M42"
                     /protein_id="HNG61375.1"
                     /translation="MTHDFFALLDILKLLVRHPSVVSAEHSFFQVLQRELEETGVKVT
                     WYEGLLVAQGNQPESRYLSAHIDRHGLICTGPNEFQYAAFIASNRGDLTGNSVSEQTF
                     ATISERFIHTAVHAYEPWSGVYRGKGLITAVYLCPQRGNMVFEVSGLEHVVAGTPVAF
                     ADKVTLLDGRISAQLDNVISAAVLIYLFRKGFQGTAFFTAQEEAGRSWRYLLEWFQRF
                     SQSTRDLLVLDTSPYPSTAEADEQLLVLRHKDSNGEFDPDTVRKLENACQILGISYGF
                     KDEYIQRKNTLRQAEGKNPLSLGSTELGRIVAASNGWVNGATVQIPTTGYHTTEETAS
                     VASIGALLQLLEHYAIHQ"
     gene            complement(2105..2644)
                     /locus_tag="PKZ52_16255"
     CDS             complement(2105..2644)
                     /locus_tag="PKZ52_16255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNG61376.1"
                     /translation="MKKLLLAAALSTFACAASADDRFYAGGNVAFWNYDEGSQDVKFN
                     VSSVEGVVGFNVWEIINIEGRLGIGLEGSTETVTSKQDGTLPTKLNLDNYASVYVKPE
                     LKNEVATFYGLLGYTSASASTENAKTERDLDVSGFSFGLGMGFYVADEVLVNIEYRKL
                     VQDSDYTFDGFSLGFTIGF"
BASE COUNT          653 a          597 c          728 g          754 t
ORIGIN      
        1 ggttgtgatg cggcgggtgc agggatagtt gttggtgatc ctaatacctt gtctaattat
       61 atagatggtg atgttgtggt tgttcctcca gcagctaatg cgaatggaaa ctatgctcag
      121 gttacgattc ccttggattg ggcaaactta ggggcgaacg gtgggacggc tgatatggtg
      181 gtaacgattt ttcgctgttc tagtggtgtt gtccgccgtt gtgcatccac ggttgctgca
      241 gactacaaat ttttccctaa cagctgccgc aacactgtcg cccagattac agcagtaccg
      301 gctggttgct agattaagaa gttgatagat aaccctgcat ttatttttgc agggttgttg
      361 tatttttcgg atcgctgatt cggtaggaag tttgttctat tgtccccggt gtgtccccgg
      421 tggtgcgtac gttagtctgt attagttcaa acttgatctg acagctaccg attttttaag
      481 gttgcctgtc agtttagatt tgagctaata cagtttagca attacgtaat ggacacattt
      541 actacttgca tagatcatct aagcgtctgt aagaattcat taggtgtata ggtaagtggt
      601 aaatggcgct gaagcacccc ttgagtattt aataatacaa tcgttggata accctgagcg
      661 ttgtatgttt gagtaaaaaa aatcgcctcc gcatccttct catcaatccg cacatagata
      721 actttctccg ttaaaaaccg cttaacttcc ggatcgctaa acacagtgcg gtccatagtt
      781 cggcaagtgc cgcaccaggt agccgataac tctgcaacaa tgaatttgtt ttcagtttgg
      841 gcgcgtttga ggccttcggc tagggtgatt ttgggcagtc ctgtgtccgc aaaatcttgc
      901 ccgccccaat aattttgtaa gcccttgacc ccgccgtaca cgatcccaag ggtaattaaa
      961 aatccccacc agctgtcggt aaaagttttg aatttattga tgaatggcat agtgctcgag
     1021 taattgcagc agggcgccaa tggaggctac ggaggcggtt tcttcggtag tgtggtagcc
     1081 ggtggtaggg atttgcactg tggcgccgtt gacccagccg ttggaggcgg cgacaatgcg
     1141 gccaagttcg gtgctgccga ggcttaaggg gtttttacct tccgcttggc gcagggtatt
     1201 tttgcgctgg atatattcgt ctttaaagcc gtaactgatg ccgagaattt ggcaggcgtt
     1261 ttctaatttg cgcactgtat ctgggtcaaa ttcgccattg ctgtccttgt gccgcaaaac
     1321 caataattgt tcatcggctt ctgcagttga gggatagggg ctggtatcta ggactaataa
     1381 atcccgcgtg gattgagaaa agcgctgaaa ccattccagt aaatagcgcc agctgcggcc
     1441 ggcttcttcc tgtgcggtga aaaatgcagt gccctggaaa cccttgcgaa acaaataaat
     1501 taatacagca gcgctgatga cgttatctaa ctgggcgctg atacggccgt ctagcagggt
     1561 gactttatcg gcgaaggcga ctggtgtgcc cgccactaca tgttctaagc cacttacttc
     1621 aaataccatg ttgccgcgct gtgggcagag gtaaacggca gtaattagac ctttgccccg
     1681 gtatacgcca gaccagggtt cataggcgtg tacggcggtg tgaataaagc gttcagagat
     1741 ggttgcgaag gtttgttcgg aaacggaatt gccggttaaa tcgccgcggt tgctcgctat
     1801 gaatgctgcg tattgaaatt cattggggcc ggtgcaaatt aggccgtggc ggtcgatatg
     1861 ggcggagaga taacgacttt caggttggtt gccttgagca accaataagc cttcgtacca
     1921 ggtaactttt acaccggttt cttcaagttc tctttgtaac acttggaaga atgaatgctc
     1981 ggcactgaca acgctcgggt ggcgaacgag cagttttaaa atatctaata aggcaaaaaa
     2041 atcgtgggtc atgtttatgc ggggcccata aaaaagggcg cttagcgccc cattttaaga
     2101 tttattagaa gccgatggtg aagccaaggc taaacccgtc aaaggtgtaa tcgctgtctt
     2161 gtaccagttt gcggtattca atattgacga gaacttcatc cgctacataa aagcccatcc
     2221 ctaagccaaa ggagaagcct gatacgtcaa gatcgcgttc agttttggcg ttttcagtgg
     2281 atgcgctggc gctggtgtag cccagtaggc catagaatgt ggcgacttcg ttctttaatt
     2341 caggtttaac gtaaacactg gcgtagttat ccagatttag tttagtaggc agggtgccgt
     2401 cttgtttgct ggtgacggtt tcggtggagc cctctaggcc aatgcctagg cggccttcaa
     2461 tattaattat ttcccaaaca ttaaagccca caacgccttc aacagaggaa acattaaatt
     2521 taacgtcctg gctgccctcg tcatagttcc aaaaggcgac attgccgccg gcgtaaaaac
     2581 ggtcgtccgc cgaggcggcg caggcaaaag tggacagggc ggcggcaagc aacagctttt
     2641 tcataccaat tcgtctccga aggtttttta ggttattttg tgcgaagtcc ttggcaggcc
     2701 taaggaggcg cgccatgagg cggggcaagt at
//
LOCUS       DAOQGS010000565         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23057_length_2731_cov_4.631913, whole genome shotgun sequence.
ACCESSION   DAOQGS010000565 DAOQGS010000000
VERSION     DAOQGS010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_2731_cov_4.631913"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..827)
                     /locus_tag="PKZ52_16260"
     CDS             complement(453..827)
                     /locus_tag="PKZ52_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61377.1"
                     /translation="MRPLINSLTQWLEQHPFAPMEIDCVTTVKLKILDGKCKMAEDEK
                     IIMGTLYDALKTRPGKLLGPEVHELIQKAHRQLDEAMRLQVYEQRLLAETMLSRPVMK
                     AFKARIRAEGLFGLLLDEPADI"
     gene            complement(831..2531)
                     /locus_tag="PKZ52_16265"
     CDS             complement(831..2531)
                     /locus_tag="PKZ52_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG61378.1"
                     /translation="MNLQLEKPYVEAMLSRFSELGFLAEQLKFGNRAEVPFQRFSDEH
                     MHYLKDLYLQAGPALRGKVAQISTLQNAICANDPKFTRGDLERLVPEITRYLTEHAER
                     GWLFKINAAGKPLAYLVTRIDFTPPGEEESGRILIDLKTHAAGKITTNTILLLDKDID
                     GQSIAQMFATKGYMRETPELLASYDASAAKYFDWRAQYGRQFTAQGTGIYAEDPTATH
                     RSQDWSRKSVVVLSSGGGCAKLVNDEEIIQNRELMLDTSGDILGKFLRKAGRNMKFSD
                     SVEKATEKAQQDIPDGLFTQLPVQGFVLMFHLELHHHLWVHVDDMQLYEYQPQLREKL
                     VLPPEQTDLIDILTAEMDVLMDDIVEGKSGGTTVLCAGPAGVGKTLTAEVYSEIIKRP
                     LYRVHSGQLGLSVAEMEKALKETLTRDQRWGAVMLIDEADVYIKRRNDNIAANAVVGV
                     FLRVLEYFNGLLFLTTNRVGDIDEAIVSRCIALIKYLPPDLADRKRIWQVMTEQFKLP
                     VAPELIDTLAERFPTACGRDIKGLSKLVAKYCQYKSAAPTLEVFERCSIFRGMDIAAA
                     "
BASE COUNT          709 a          648 c          660 g          714 t
ORIGIN      
        1 ggagtgattg aaatttgacc taattcgcag aaaggtcaga caatataaca gacggttgaa
       61 tataagccaa aaaatcgcgc ctaaatgatg caaaggatct aagcaaaacc gtgcgaatgc
      121 tacggcgatt aaatcgctaa atgcgcaagt cctataagta aacaaaaccg aggggcggcg
      181 ttgatcagta gaaataataa aaatccacct taaatttatt ttgacattat ggggtgcaat
      241 ttcaggaggt tattaactcg gcactaaatg ccgcagggtt aataatatag gcaggaggtt
      301 tcaaggtcca agcgataaag cattgcagtt ttgcgcacag gcgaatgaaa ctaaagcggc
      361 gatttgtagc aaaagtattt aaaaagacta ttaacagctt gttgaaaaac taccttgcca
      421 gagcgtcgcc gcctgcgtat ctcaacaagc tgctaaatat cagcaggctc atccagcagc
      481 aggccaaata atccttcggc gcgaatgcgg gctttaaagg ccttcatgac cgggcgggag
      541 agcatggttt ccgccagaag tcgctgctca tacacctgca ggcgcatggc ttcgtccaac
      601 tgacggtgtg ctttttggat taattcatgt acctcaggcc ccaacagttt gccagggcgg
      661 gttttaaggg cgtcatatag agtgcccatt attatttttt cgtcctcagc cattttgcat
      721 ttgccatcga ggatttttag cttcacggtc gtgacgcaat caatttccat cggcgcaaac
      781 ggatgctgct ctaaccattg cgtcagtgaa tttatcagag gtcgcatagg ttaagccgcc
      841 gctatatcca tacccctaaa aatactgcag cgctcaaaca cctctagggt tggagcggcg
      901 gatttgtatt ggcaatattt ggccaccagt ttactcagac cttttatatc ccgcccgcag
      961 gcggtgggga aacgctccgc cagcgtgtcg attaattccg gcgccacggg cagtttaaat
     1021 tgttccgtca ttacctgcca aatacgtttg cgatcggcca aatcaggcgg caaatatttg
     1081 atcagtgcaa tgcagcggct gacgatggct tcgtctatat cgccaactcg gttagtggtg
     1141 agaaataaca gcccattaaa atattccaat acgcgcaaaa atacgcccac aacagcattg
     1201 gccgcgatat tgtcgttgcg ccgtttaata taaacatccg cctcatcaat taacatcaca
     1261 gctccccagc gctggtcgcg ggtgagggtt tctttaaggg ctttttccat ttcggccaca
     1321 cttaggccca gctgcccgga gtgtacgcga tataaaggcc gtttaattat ttctgaataa
     1381 acttcagctg ttagggtttt gcccaccccc gcaggccctg cacataacac cgttgtgccg
     1441 ccggatttac cttccactat atcatccatt aatacatcca tttcagcggt gagaatatcg
     1501 attaaatccg tctgctccgg cggtaatact aatttttccc gcaattgtgg ttggtactcg
     1561 tataactgca tgtcatcaac gtgcacccag aggtgatggt gcaattctaa gtgaaacatt
     1621 aaaacaaacc cctgcaccgg cagttgggtg aacagcccat cgggaatatc ctgctgggct
     1681 ttttctgtgg ctttttccac cgagtcgcta aatttcatat tgcgcccagc tttacgcaaa
     1741 aacttaccga gaatatcgcc gctggtatcg agcattaatt cgcggttttg aataatttct
     1801 tcatcattga ctaattttgc acagccgccg ccggaggaga gtaccaccac actcttgcgc
     1861 gaccagtcct ggctgcggtg agtggcggtg gggtcctcgg catagatgcc ggtgccttga
     1921 gcggtaaatt ggcggccgta ctgagctcgc cagtcaaaat atttggcggc cgaggcatcg
     1981 tagctggcga gtaattctgg cgtctcccgc atataaccct tggttgcaaa catctgggcg
     2041 atgctttgac cgtcaatatc tttatccaag agtaaaatgg tgttggtagt aattttgccc
     2101 gctgcgtggg tttttaaatc gatcaatatg cggccggact cctcctcccc cggcggcgta
     2161 aaatcaatgc gggtaactaa atacgcaagg ggtttacctg cggcatttat tttaaataac
     2221 cagccccgtt cggcatgctc tgtaaggtaa cgagtaattt ccggcaccaa gcgctcaaga
     2281 tcgccgcggg taaatttggg gtcgttggcg cagatggcat tttgcaatgt gctaatttgt
     2341 gccaccttgc cgcgtaaggc agggcctgct tgcaaatata gatctttaag gtaatgcata
     2401 tgctcatcgg aaaagcgctg gaatggcacc tctgcgcggt taccgaattt taactgttca
     2461 gctaaaaagc caagctcaga aaagcgactt agcatggcct cgacataggg tttttctagc
     2521 tgcaggttca tagatgcctc atgattaaaa agcgtgcgct caatggatcc ctaaaatgag
     2581 ctgaatagtt aagggattgt ctgctttgtt gcaattgtcg tgaaagtaac aggcagcctt
     2641 gggtttagtt catgcggtgt gcggcagtta ccctcaatag tttgagtgac cagacaaaag
     2701 ttagagtgac ccaatggtta gacagatcag g
//
LOCUS       DAOQGS010000566         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23084_length_2729_cov_6.483545, whole genome shotgun sequence.
ACCESSION   DAOQGS010000566 DAOQGS010000000
VERSION     DAOQGS010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23084_length_2729_cov_6.483545"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(825..1655)
                     /locus_tag="PKZ52_16270"
     CDS             complement(825..1655)
                     /locus_tag="PKZ52_16270"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG61379.1"
                     /translation="MSFDQLFENATRLPSIPKVVQELLQTFDNPDVDMKQIANKIALE
                     PVLTAKLLRTANASHYGLPRKVASVHDAVIVLGFSAVRTLVMASGLVTSLQMPAALDK
                     KQFWTDAFAVAEGAKWLGKFGARVDANTLATAGLVHRVGQLLMYSVYPELAAKVKHAI
                     AAGQSRLEAEQAVFGYTYCVVGAELAKRWRFPAVICDALLHQNAPLAASPFSLLGAYL
                     HLAKYMHSCLQAGVSVEELIEHFPTAVSDACDINKEKLFAALSDFKGLASAMDSMLDD
                     "
     gene            complement(1904..2059)
                     /locus_tag="PKZ52_16275"
     CDS             complement(1904..2059)
                     /locus_tag="PKZ52_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61380.1"
                     /translation="MINRMDFFALLFFQIAITFALAQVLAKRGCADCDKSAAEPAVFD
                     QLVLVDH"
BASE COUNT          756 a          715 c          627 g          631 t
ORIGIN      
        1 acctctgaaa aatgtaggcg gcgaagctct ggctaggcga atttggcaaa aaaagtggac
       61 gactgcaggg aggcaggagg tagagcaatg caggagcaat tgccgagttt acacgcagta
      121 aatgagcatt tttttgccaa attcaacgca gccagggcga gcaaggtaat ttttcagagg
      181 ctccttaggc aaaaccacag cgaaaagtcg tagcctgttg tcagcacgac tggacacagg
      241 ttatttaagg ccgttaattg gatttaaata tccgactgtg cgcaggataa cgctgcgaac
      301 ccccgctcat tcaatccctg caaaacagcc cttaaatcgt tgctcttcag cagtttttat
      361 tagcgctgga ggtggttctc gtgccggcta acaaacgccg ctcaagcact taagtactgc
      421 gagtgccctc ggtgtaaata gttaggttat tatcccggcg gcaaaataaa cctacgaaaa
      481 tgcgcggatt ttcttgtttt agtgcagcta gctgcggttt acactgccga ggtggcatcc
      541 ttgccgcctt tattaacccg gcactaattg ccgccgggtt aataaatata accattcggc
      601 acaccataat ttattttatc tgcattttct tcgcttgaag tgaggaaaaa agaggtcggc
      661 cctagcgagg ccggagcgct tattaaagga ggcatgtacg gatatatctt ggccaggact
      721 aaaaggcgcg aaaataatta cagtaaattt atctacgcgc aaaaataaaa aactcggcgt
      781 gtgcggccga gtttttcgct aaaaaggtag caggcgccct tgcattaatc gtccaacata
      841 gaatccatcg ccgaggcaag gcctttaaaa tcgctcaaag cggcaaagag tttttcttta
      901 tttatatcgc aggcatcact cacagcagta gggaaatgtt caatcaactc ctccacggat
      961 acaccggctt gcaaacacga gtgcatatac ttagccagat gcaaataagc gcctaacaac
     1021 gaaaaagggc tcgccgccag aggggcattt tgatgcaata aggcatcgca aatcaccgcc
     1081 ggaaaccgcc aacgtttggc gagttccgcg cccaccacac agtaggtata accaaatacg
     1141 gcttgttctg cttccagtcg cgactgcccc gccgctatag cgtgttttac ttttgcggcc
     1201 agttccggat acaccgaata cattaacaat tgccccacgc ggtgcactaa gccagcagtg
     1261 gcaagcgtgt tagcatccac ccgcgcgccg aatttaccca accatttagc cccttcagca
     1321 accgcaaacg catcggtcca aaattgtttt ttgtccagcg ccgcaggcat ttgtaagctg
     1381 gttactaaac cagaagccat caccagggtg cgcacagcgg aaaaccccaa cactatcacc
     1441 gcgtcgtgca ccgaggccac cttgcgcggc aggccgtaat gagaggcgtt ggcagtgcgc
     1501 aataatttgg cggtcagcac cggttcaagg gctattttgt tggcgatttg tttcatatcc
     1561 acatcgggat tatcaaacgt ctgcaacaac tcttggacga ctttgggaat ggacggcaaa
     1621 cgggtagcgt tttcgaataa ctgatcaaag ctcatagtgt aaatctcctg acactctaaa
     1681 caatgatagg ccaccggcgt aaattcgcta aaaataggca aaaaaaaggc gccttgcggc
     1741 gccaaaacac acataactaa tttctctgct ggttagttaa cgcacgcaag cgcaacgaac
     1801 ctaccaatac atttactcaa acccagtttg cagaagcctc atctccagtg gctacccaat
     1861 ttcgcctaca cagctgctac cacacagccg tataaccagc taattagtga tccaccaata
     1921 ccaactgatc aaataccgca ggctccgctg cactcttgtc acagtcggca cacccgcgtt
     1981 tagccaacac ctgggccaac gcaaacgtaa tggcgatctg aaagaataat agtgcaaaaa
     2041 aatccattcg gttaatcatc ttcacaccct caataacaca ctttcggccg gccacgccct
     2101 gcacactgca agccttggcg aaccgaaaac aagccgtaat tgttggcgcc cgctgatacc
     2161 tctggggcga actatcggga ggagtatgcg ggaagatcag tttttcctca gtaatcgagc
     2221 cttgggttga tatgtttacc ccgtataaac acacccgctc gcagcttgat cactgggaaa
     2281 aactgagctt cccacagccc cctcgaagtg aatgtttctc acatccaccg atgcaaccct
     2341 acatagcaaa cataaatcca tcataaattt ttcttttaat atcataatgt tatgagatac
     2401 aaaggccgcc acccaagcac gcaagacaag gtattttgtt gccatttggc aacaggcttg
     2461 ctgagcaata gaaataactt ataaaaatca aatagttata gtgttgccaa aattccacgc
     2521 ggcaaagatg ctagactttt ggcccttttc actggagagc ttggcgtgtt gcgtaggtgg
     2581 atcaatcggc tgcggctcac ccatcaatgg gtcatagcga cgctgctggc gctcttgccg
     2641 ctgacggctg ccgtgattta tggcggctgg tccatctggg cgcagtccca cgatcagcgc
     2701 atgttggtgg cccacctgca acaggtggg
//
LOCUS       DAOQGS010000567         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23176_length_2723_cov_3.727136, whole genome shotgun sequence.
ACCESSION   DAOQGS010000567 DAOQGS010000000
VERSION     DAOQGS010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23176_length_2723_cov_3.727136"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKZ52_16280"
     CDS             complement(<1..200)
                     /locus_tag="PKZ52_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61381.1"
                     /translation="MNRLFLHFSAYFIITSGSIALASDTLPMKFSEDNSISAEMDSSS
                     YLKSIPIQNECTEEKSRELTVGT"
     gene            complement(446..2377)
                     /gene="acs"
                     /locus_tag="PKZ52_16285"
     CDS             complement(446..2377)
                     /gene="acs"
                     /locus_tag="PKZ52_16285"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNG61382.1"
                     /translation="MSNPLYAPAAHLAQSAHINAEQYQTLYAQSIQNPDAFWAEQARR
                     FISWQRPFSETFRGNFAEGEVSWFGDGTLNLAENCIDRHLPTRANQTAIIWEGDNPSE
                     SKHISYQELHDQVGRLSNLLKNKGVKKGDRVCIYMPMIPEAAYAMLACARIGAIHTVV
                     FGGFSPEALKDRILDADCKIVITADEGLRAGKTIPLKNNTDRALDFCPQVKTVLVVKR
                     TGGAINWFDNRDVWYSEAENLSPICPAEEMTAEAPLFILYTSGSTGKPKGVLHTSAGY
                     LLQAAMTHRYVFDYQDGEIYWCTADVGWVTGHSYIVYGPLCNGATTLMFEGVPTYPDA
                     SRCWQVIDKHQVNIFYTAPTAIRALMALGDEPVKRCSRASLRILGSVGEPINPEAWQW
                     YYQVVGDSRCPIADTWWQTETGAHLITPLPGAHTLKPGSASLPFFGVEPGLVDSNGQL
                     IEGPGEGNLVIKRSWPSQIRSVYGDHERMVQTYFATYPGFYFTGDGARRDEDGYYWIT
                     GRVDDVLNVSGHRLGTAEIESALVLHPKVAEAAVVGYPHDIKGQGIYAYVTLMQGEVG
                     DEALIKELVALCNKEIGPIAKPDLIQWAPGLPKTRSGKIMRRILRKIAANEVDSLGDT
                     STLADPSVVESLIGSRLNK"
BASE COUNT          606 a          646 c          759 g          712 t
ORIGIN      
        1 gtgcccactg taagttccct agatttttct tctgtacatt cattttgaat ggggatgctc
       61 tttaaatatg aagagctatc catttccgca gaaatgctgt tatcttctga aaatttcatc
      121 ggcaatgtat cggaagccag cgctatgctc ccggaagtaa taataaaata agctgaaaaa
      181 tgtaagaata atctattcat gatctgatcc tggttggttt tgcattcgag accaatctta
      241 atgctataaa attgtgttgg tcaactgtaa gatttagttt agcaactaac aagtatcctg
      301 gatattcgga tttacgctgg gcgggttcgg agcgaagcga caatccactt tttgccgccc
      361 caattttgcc gattattggc aggctatgat gttattgggc acaagccatt tgattgcagc
      421 gataaaagaa gcgcagtacc ccctcctatt tattcaaccg agacccaatc aaagactcca
      481 ccacactagg atccgcaagc gtagaagtat ccccaagcga atccacctca ttagccgcaa
      541 ttttccttaa aatcctgcgc ataattttcc ccgaacgcgt tttgggcaac cccggcgccc
      601 actgaattaa atccggctta gcaatcggcc caatttcttt attacatagc gcaaccaatt
      661 ctttaattaa tgcctcatcc ccaacctcgc cctgcattaa agtcacatag gcataaatcc
      721 cctggccttt aatatcgtgg gggtaaccca ccactgccgc ttcggcaact ttggggtgta
      781 ataccagggc gctttcgatt tcggcggtgc ctaggcggtg gccgcttacg tttaatacgt
      841 catctacccg gccggtaatc cagtaataac cgtcttcgtc gcggcgggcg ccgtcgccgg
      901 tgaagtaaaa tccggggtag gtggcaaaat acgtttgcac catgcgttcg tggtcgccat
      961 agacgctgcg aatttggctg ggccaggaac gtttaatgac taaattgcct tctcctgggc
     1021 cttcaattaa ttggccgttg gaatccacta ggcccggctc tacgccaaaa aagggcaggc
     1081 tggcggagcc gggttttaat gtgtgggcgc cggggagggg ggtgattaaa tgggcgccgg
     1141 tttcggtttg ccaccaggtg tcggcaatgg ggcagcggct gtcgccgact acttggtagt
     1201 accactgcca ggcctctggg ttaatgggct cacccactga gcctaaaatg cgcagggagg
     1261 cgcgggagca gcgtttgacg ggttcatcgc ccagggccat aagtgcgcgg atggcggtgg
     1321 gggcggtata gaagatattg acttggtgtt tgtcgatgac ctgccagcag cgggaggcgt
     1381 ctgggtaggt gggtacgcct tcaaacatta atgtggtggc gccgttgcat agggggccgt
     1441 agacgatata gctgtggccg gttacccagc ctacgtccgc tgtacaccaa taaatttcgc
     1501 cgtcttggta gtcgaacacg tagcggtggg tcatggccgc ttgtaataaa tatccggcgc
     1561 tggtgtgtaa tacgcctttg ggtttaccgg tggagccgga ggtgtacaaa ataaatagcg
     1621 gcgcttcggc ggtcatttct tcggcggggc aaatggggct taaattttcg gcctcgctgt
     1681 accacacatc gcggttatca aaccaattaa tggcaccgcc ggtgcgtttt accactagta
     1741 cggttttaac ttgggggcaa aaatccaggg cgcgatcggt gttgttttta agtggaattg
     1801 ttttgcctgc gcgcaggcct tcatcggcgg tgataacaat tttgcagtcg gcatcgagga
     1861 tgcgatcttt taaggcttct ggcgagaagc cgccaaaaac tactgtgtga atagcgccga
     1921 tgcgggcaca ggcgagcatg gcgtaggcgg cttcgggaat catgggcata tagatgcaca
     1981 cgcggtcgcc ttttttcacg cctttatttt ttaataaatt actcaggcgg cccacttggt
     2041 cgtgtaattc ttggtagctg atgtgtttgc tttcgctggg gttgtcgcct tcccaaataa
     2101 tggcggtttg gttggcgcgg gtgggtaaat ggcggtcgat gcagttttcc gccaggttca
     2161 aggtgccatc gccaaaccag ctcacttctc cttcggcaaa gttgccgcga aatgtttcgc
     2221 taaaggggcg ctgccaggaa ataaaacggc gggcttgttc agcccaaaag gcgtcggggt
     2281 tttggatgga ttgggcgtag agggtttggt actgctcggc gtttatgtgg gcggattgcg
     2341 cgaggtgtgc agcgggtgca tacaaagggt tcgacatggc agctccagtt ttttattggt
     2401 gttgtgaacg agtgaatatg ttagggatgc catcatactt gagggggctt ggctgggtac
     2461 agcaactaaa cgctcggtaa tacccggctg ttttgcgcag cggctgtgcc tactggggtg
     2521 ggcgttttgc gtaggtggct gcaggtggca cactaagatc accccgcccc tgggcaatgt
     2581 cattaagtta aggttttcgc actatttcca cagaaaattt tctctcatcc tgaaacccca
     2641 ccccaaccct ccccttaaaa aggggaggga gctgagcagc ctgaaaacag gtaaaattcc
     2701 ttgtgttgaa ggtttactgc ccc
//
LOCUS       DAOQGS010000568         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23365_length_2710_cov_4.212429, whole genome shotgun sequence.
ACCESSION   DAOQGS010000568 DAOQGS010000000
VERSION     DAOQGS010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23365_length_2710_cov_4.212429"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKZ52_16290"
     CDS             <1..293
                     /locus_tag="PKZ52_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61383.1"
                     /translation="HSDQPPAVKITAIGRMQQYDAINSTELLLTALDDSAPEVSVIAL
                     NTLAAQADESLLSRIKEKMFGLPDGTIRNFYLETIDRIEIRLTLGYSDLTRQ"
     gene            complement(436..978)
                     /locus_tag="PKZ52_16295"
     CDS             complement(436..978)
                     /locus_tag="PKZ52_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNG61384.1"
                     /translation="MAQIGVFFGTNTGKTRKVAKMISGKFGDDIMSKPINVNRSNLEE
                     FLSYDYLILGTPTLGEGLLPGMSADCDGESWEEFLPKLEGADFSGKTVAIYGLGDQVG
                     YAHEFVDAMAELYEFMLDKGAKVVGAWPTDGYEFDHSEAVVDGKFVGLALDLDNQSGL
                     TEQRVDAWLKLIAADFKLPL"
     gene            1765..2028
                     /locus_tag="PKZ52_16300"
     CDS             1765..2028
                     /locus_tag="PKZ52_16300"
                     /inference="COORDINATES: protein motif:HMM:NF016226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNG61385.1"
                     /translation="MSLAHLHNGDVVYARIDIRNDGSVPDADTDALFATAGAKGMLLN
                     IGHFEENPNVELFLVTFEDANGELGPPVTCLAEEISAAPVSLA"
     gene            2039..>2710
                     /locus_tag="PKZ52_16305"
     CDS             2039..>2710
                     /locus_tag="PKZ52_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNG61386.1"
                     /translation="MSAPAEQDDALNQVRRYHQATKHFPNAYARGPGSLDWDAQPNPF
                     RHFSGCEQVPLPLQADRQEQTEGLEQPFAALYQTRQPAHNMNLAGLACVLELSLALSA
                     WKQYGPSRWALRCNPSSGNLHPTEGYALVVNLPGLADGVYHYNAEHHSLELRCRFQEQ
                     AAPTLSPQLLIGLSSVYWREAWKYGERAYRYCQLDVGHALGALSYAAQLQGWPCYLMS
                     EASSQD"
BASE COUNT          712 a          736 c          639 g          623 t
ORIGIN      
        1 tacacagcga ccaaccaccc gccgtgaaaa ttacggccat tggccgcatg caacaatacg
       61 acgctataaa ttcaacagaa ttattgctta ccgcactaga tgatagcgcg cccgaagtat
      121 cggtgattgc ccttaataca ctggcagccc aagcagacga aagcttgctt agccgcatta
      181 aggaaaaaat gttcggcctt ccagatggta caatcagaaa tttttacctg gagactatag
      241 atcgcatcga aatacgcctt acactggggt atagcgattt aacacggcaa taggagcagc
      301 tcttgtttag gctgaaggtg ggaatagtgt gaaccctcgc gggcagaaga cccgcataag
      361 ctatagggat gcacctatgg gttttgacca atttaaaacc aacagagcat ccccatccat
      421 tgggcattca ataacttaca gcggcaattt aaaatccgcc gcaatcagtt ttagccaagc
      481 atccacgcgc tgttcggtaa ggcccgattg attgtccaaa tccagcgcca ggccaacaaa
      541 cttgccatct actacagcct cagagtgatc aaattcatag ccatcggtgg gccaagcgcc
      601 aaccacttta gcgcccttat ccagcataaa ttcatacagc tctgccatgg catctacaaa
      661 ttcgtgggcg taacccactt ggtcgccaag gccgtaaatc gcaacggttt taccggagaa
      721 atcggcacct tctaacttgg gtaaaaattc ctcccagctc tcgccatcac agtcggcaga
      781 catacccggc aacaggcctt cacccagggt gggggtacct aaaattaaat agtcgtagct
      841 taaaaattct tctaagttac tgcggtttac atttataggc ttggacataa tatcatcgcc
      901 gaatttaccg ctaatcattt tcgccacttt gcgtgttttg ccagtattag tgccaaaaaa
      961 taccccaatc tgtgccatga gttgttcctc tttaaaagtc aagaatgcaa ataagtcaat
     1021 aaaacaggtg taaccaaatg cgatgttaag gccactgtct gtggcgctta aaaagggtta
     1081 atcgttgctg accggttttg cgaaacttat gccacctaag gaacattcaa ttagaaccgc
     1141 caagcgcttg ccataaatta tccacagcgg gcagtataaa aataatcact gaatagccag
     1201 ccagcgatta aaaacccagt gctagcaggg ctcgttgcaa gtaaccaccg gaatttttgt
     1261 cgcaaatacc acagcatttg tacaaaagag tttttaaggg aggagatttt gtccggaata
     1321 agacaaacta ttttcatccc tgctgagcaa atgctgtaga ttcccaacat ggctgcagat
     1381 tggcaaagcc cttgctcatg gttttatcat tagttcagat tcaaccctcg cttatttaat
     1441 cagcagcgtt ctatgccggg taagtccagc atggatgccg ccgcaggctg cgcttaaacg
     1501 agaaaaccca catttgttcg caattattaa cccggcggca tttagtgccg ggttaataat
     1561 tgcggcatgg atgccgccct ggctgtgcgg gccgccgcga gctgcactaa aacaagaaaa
     1621 tccgcgcatt ttcgcaagtt ttagtgcagc gaagtgacgg cagaacagat cggaacggcc
     1681 tattccaccg ctgggttaaa ggatcggtaa atattcacgg cggcagattt aaacagaaca
     1741 tcctcaactc aacgtggtaa tcctatgagc cttgcccacc tgcacaatgg cgatgttgtt
     1801 tacgcccgca tagatattcg caacgacggc tcggtgccgg atgcagacac ggatgcctta
     1861 tttgcaacag ctggcgccaa aggcatgttg ttaaacatcg gccattttga agaaaacccc
     1921 aatgtagaat tatttttggt gacctttgaa gacgccaatg gcgagcttgg gccaccggtt
     1981 acctgcctgg ccgaggaaat cagcgccgcc ccagttagcc tcgcatgagt gttcttgcat
     2041 gagcgcccct gcagagcagg acgacgccct caatcaggtt aggcgttacc accaggctac
     2101 taaacatttc cccaatgcct acgcccgcgg tcccggcagc ctggattggg atgcccaacc
     2161 caatcccttc cggcacttta gcggttgcga gcaggtgcct ctgcccctac aggctgatag
     2221 gcaagagcag accgaagggt tagaacagcc ctttgcagcc ctctaccaaa ccaggcagcc
     2281 cgcgcacaac atgaatttag cagggcttgc ctgtgtacta gagttatccc tcgccctttc
     2341 cgcctggaaa caatacggcc cctcccgctg ggctctgcgc tgcaatccct ccagcggcaa
     2401 tctgcacccc actgaaggct acgccctggt ggtgaacctg cctggcctcg ccgatggggt
     2461 ttatcactac aatgcagagc accacagcct ggagttgcgc tgcaggtttc aagagcaagc
     2521 cgctcccacc ttatcgcccc agctgctgat tggccttagc tccgtgtatt ggcgggaggc
     2581 atggaagtat ggggaacggg cctaccgcta ttgccagctt gatgttggcc acgccctagg
     2641 ggcgctcagc tacgccgccc agttgcaggg ctggccctgt tatttaatga gtgaagccag
     2701 cagccaggac
//
LOCUS       DAOQGS010000569         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23589_length_2694_cov_4.708223, whole genome shotgun sequence.
ACCESSION   DAOQGS010000569 DAOQGS010000000
VERSION     DAOQGS010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23589_length_2694_cov_4.708223"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..800)
                     /locus_tag="PKZ52_16310"
     CDS             complement(117..800)
                     /locus_tag="PKZ52_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNG61387.1"
                     /translation="MQPTYSIGSKEIIASQTHKVLRNTYSFLACTLVFSALTAAASMA
                     LGVGHGLALVSNLIALALLWFVLPRTAQSGAGVGVVFAITGLLGAGIGPMLGHYLALP
                     HGGLLVMQALAGTALVFFALSAYVLTTGKDFSFMGGFLMVGLISVLITIVGSMVLGLF
                     GVQISGLAMAINGVIVLLMSGFILYDTSRIIHGGETNYVMATVALYLDILNLFTSLLQ
                     LLGFFSNDD"
     gene            complement(1305..2069)
                     /locus_tag="PKZ52_16315"
     CDS             complement(1305..2069)
                     /locus_tag="PKZ52_16315"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNG61388.1"
                     /translation="MTSPYIPPEAELNYNAFQAHSAGVHAPASLPAGRGLDWIKEGFS
                     YFKQDAGTWILITIVGAVIFVAMNTIPIVNIFAGFFTYVFSGGLMLGAKAQRDGQPLN
                     IGHLFAGFQNSLGSLLGLGGIVMGYTVIAVIIAFAGLMLKITEFNADFLQQLLTGQGI
                     FLSVAILLVLLTPLFMGVWFAPALIVINKIPVLQAITLSIQGCLKNVLPLTINGLLIG
                     LLFFVGAIPLMLGLLVAVPVFYISVFIAYKEIYIDP"
     gene            complement(2281..>2694)
                     /locus_tag="PKZ52_16320"
     CDS             complement(2281..>2694)
                     /locus_tag="PKZ52_16320"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNG61389.1"
                     /translation="YGSLMFPAVWQQLVPERRPHRAAFLPHYSRRLIFLDTYPLIIPD
                     ATSPGILGTLYSGLTAEDIKRLDWFEGELYQRINLEVRVKTGLIACETYVPKPAYSAL
                     AMQDSWRGDIFQQIQMPIFLARYCAKNLPTDTAAG"
BASE COUNT          768 a          631 c          685 g          610 t
ORIGIN      
        1 gatacaggcg cgcgcgaact caagcccgtg gagggcagca gcgctaaagg gagcggcttt
       61 tatacagagg ctaaaggagg ccataaaaca aaaggcccct tgcggggcct caaccattag
      121 tcgtcgttac tgaagaaccc cagcaactgc agcaggctgg tgaagagatt caaaatatcc
      181 agatacaaag cgacggttgc cattacatag ttggtttcac cgccgtgaat aatgcggctg
      241 gtatcgtaca gaataaaacc cgacatcagc agcacgatga cgccgttgat ggccatggcc
      301 aatccggata tttgcacacc gaagaggccg agtaccatgg agcccacgat ggtaatcaac
      361 acactgatca ggcccaccat caaaaaaccg cccataaagc taaagtcttt gccagtggtt
      421 aacacatagg cagagagggc aaaaaatacc agggcggtgc ccgccagggc ctgcatcacc
      481 aacaaaccgc cgtggggcaa ggccagatag tggcccagca taggcccaat gcctgcgcca
      541 agcagccctg tgatggcaaa caccaccccc acacccgcgc cactttgcgc ggtacggggc
      601 aatacaaacc ataacagtgc caaagcaatt aaattactca cgagcgcaag cccatggcca
      661 acccccagcg ccattgatgc agccgctgtg agcgcgctga acaccagtgt gcaggcgagg
      721 aagctgtagg tattgcgcag aaccttgtgg gtttgggaag caataatctc tttgctgcct
      781 atggaataag tgggttgcat aaggattctc cgttaaagtt ggatagttca atggtacttg
      841 ggacaagcgc aaaagcaaag gttccacgcc ttttcgccac ctgttaaagc ctagggtagc
      901 cgctatccct atgagcacct agcgttttaa tttggggatt attgcggcga tttctaggga
      961 acctctgaaa aatgtcggcg gcgaagctct ggctaggcga atttgccaaa aaaagcggag
     1021 tttacatgca gtaaatgagc atttttttgg caaattcaac gacgccagag cgagtaaggt
     1081 aatttttcag aggttcccta gttgtaccta ggccaaaaag tgtcaggaat tcgctaaatg
     1141 gccactgcag tttaggcgcg tacaatgggg tgtattaagg tggctctgat cgagtctgaa
     1201 ttcgtaagta ttctacaaac gcaccggtgc cttcccgtag ctttcacgcg agagtcctta
     1261 tcggtcgcca tttcagccgc aattgtcctg aagttcccta agggttaagg gtcgatatag
     1321 atttctttat aggcgataaa tacagagata tagaacacgg gtaccgccac taataaaccc
     1381 agcatcagcg ggatggcccc cacgaaaaac aaaaggccta taagtaagcc atttatggtc
     1441 aggggtaaaa catttttcaa acatccttga atgctcaggg taattgcttg caatacaggt
     1501 attttgttaa tcacaattaa agcaggggcg aaccagacgc ccataaatag cggtgttaac
     1561 aggactaaaa gaatagccac cgatagaaaa atgccttggc cagttaataa ctgctgcaaa
     1621 aaatcagcat taaattccgt gattttgagc atgaggccgg caaaagcgat aataacggca
     1681 ataaccgtgt agcccatcac aatgccacct aggcctagaa ggctacccaa tgaattttga
     1741 aaacctgcaa atagatgccc gatattaagt ggctgtccat cccgttgtgc tttggcgccc
     1801 agcattaagc cgccactaaa cacataagtg aaaaaaccgg caaaaatatt cacgatcggg
     1861 atggtattca ttgccacaaa aataacagca cccacaatgg taattaaaat ccaggtacct
     1921 gcatcctgct taaagtagct aaagccctct tttatccagt ctagtcctcg cccggcgggc
     1981 aggctggcgg gtgcgtggac acctgcgctg tgggcttgaa aagcgttgta gtttaattca
     2041 gcttctgggg ggatataagg gctggtcatg tgcctgatcc tcgtggcgtt attattggcg
     2101 gacaacagtt ggcacaggtt agccttaaat agaccgtgtg accagcggat acagtgctat
     2161 cgcctgggta tttaaacgag ctgctgggga tgcgttaatt aagtctgcat tcgcgaatag
     2221 tgtaaaaaga cacattgagc agaaatgtgt cggttaaaag ttgtgttgta aaacggcaac
     2281 ttaacctgca gctgtatccg taggtaggtt tttagcgcaa taacgagcta aaaatatcgg
     2341 catttgtatc tgctgaaaaa tatccccgcg ccaggaatcc tgcatggcaa gagcgctata
     2401 ggctggttta ggtacatagg tttcgcaggc gattaggccg gttttaaccc gcacctctag
     2461 gttgatgcgt tgatatagtt ccccttcaaa ccagtcaagt cgttttatat cctcggctgt
     2521 taagccgctg taaagagtgc ccagaatacc tgggcttgtg gcgtcgggga ttatcaacgg
     2581 ataggtgtca agaaatatta ggcggcgact gtagtggggt aaaaaagctg ctctgtgtgg
     2641 gcgccgctca ggaaccaatt gttgccatac ggccggaaac attaaagagc cgta
//
LOCUS       DAOQGS010000570         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23750_length_2681_cov_4.775324, whole genome shotgun sequence.
ACCESSION   DAOQGS010000570 DAOQGS010000000
VERSION     DAOQGS010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23750_length_2681_cov_4.775324"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKZ52_16325"
     CDS             complement(<1..574)
                     /locus_tag="PKZ52_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61390.1"
                     /translation="MYCLIRVNAHPQLGNGHLMRCLTLARIAHGRGYKPVLVLSDLPG
                     VPTGFIREAGFNYVLLPAANETQDATNFKALIQDLDAPCRVVVDVYAFAAIWHRIVYP
                     LCEYLLVIDDLANRTYCCDVLLDQTPGRSADDYKPLVNNAAQVYAGCEHALLAPEFFA
                     AVNDAKRVREHYCAQKRLPRLLVSLGGTDPK"
     gene            complement(553..1263)
                     /gene="pseF"
                     /locus_tag="PKZ52_16330"
     CDS             complement(553..1263)
                     /gene="pseF"
                     /locus_tag="PKZ52_16330"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014975890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNG61391.1"
                     /translation="MRVAVIPARGGSKRIPRKNIKPFLGQPIIHYAIKAALESELFDL
                     VLCSTDDAEIAQVAIGAGAQVPFVRPADIADDHAITADVIAHAISFLQSNGQPVESVC
                     CLYATAAFVTAEQLQQACTVFERERCDFVFSATAFDFPIQRAIYLTAEGRVEMFYPEY
                     ALTRSQDLTPAFHDAGQFYWGSATAWLEKRRIFSPNSAAFIVPSHEVQDIDTLEDWRR
                     AELLYQLRQQLLCTASSA"
     gene            complement(1254..2390)
                     /gene="pseC"
                     /locus_tag="PKZ52_16335"
     CDS             complement(1254..2390)
                     /gene="pseC"
                     /locus_tag="PKZ52_16335"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HNG61392.1"
                     /translation="MIPYGRQHISQLEIDAAREVLQSDWLTQGPKVVEFEQALAAYVQ
                     APHAVTCSNGTAALHAACAALGLGPGDELWTSPNSFVASANCGLYLGAKVDFVDIDPV
                     SRNMCPQQLAAKLAKGRVPKIVVAVHFAGVACDMPAIFALAQQYGFYVVEDAAHAIGT
                     QVNNVPVGACRFSHATCFSFHPVKNLTTGEGGAITTADSVIANRLRRFVTHGITKNPQ
                     EFTDPNPPPWYYEQQSLGFNYRLCDIQAAVGVVQLGKLETFIDKRRQLFERYQTLLAK
                     LPIALPHVPSRQRVAHHIYAVGVDPQLRLTVFNALKSSGIGVNVHYIPIHLQPFYQAL
                     GFKAGDYPLAEAYYAGALTLPLFVEMTFAQQDYVLEQLERNLCA"
     gene            complement(2387..>2681)
                     /locus_tag="PKZ52_16340"
     CDS             complement(2387..>2681)
                     /locus_tag="PKZ52_16340"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNG61393.1"
                     /translation="LAEAIAPEMPTKIVGIRPGEKLHETMCPLDDSHLTLEFDDHFVI
                     TPTIKLFRYVNYEINNLGERGHTVPRGFSYNSGNNPHVLNVDEIRQLHEMAEV"
BASE COUNT          742 a          693 c          631 g          615 t
ORIGIN      
        1 ctttagggtc cgttccccct aaggaaacca gtaatctagg cagccgcttt tgtgcacaat
       61 aatgctctcg cacgcgctta gcatcattca ccgccgcgaa aaattccggc gctaataagg
      121 cgtgttcaca acctgcataa acctgcgcag cattgttaac taagggctta taatcatcgg
      181 cgctgcgccc aggggtttga tccaacaata catcacaaca ataggtgcga ttggctaaat
      241 catcaatgac caataaatac tcgcaaagag gataaacaat gcggtgccaa atagcagcaa
      301 aggcatatac gtccaccacc acccggcagg gcgcatctaa atcctgtatt aatgccttaa
      361 aattcgttgc atcctgagtt tcattggcag caggcaataa cacataatta aacccggcct
      421 cgcgaataaa acctgtgggc acacccggta aatcgctcaa caccaataca ggtttatacc
      481 cgcgcccatg ggcgatacgc gccagggtta aacagcgcat taaatggcca ttacccaatt
      541 gcggatgcgc gtttacgcgg atgaggcagt acataataat tgctgccgta attgatataa
      601 tagttcagca cgccgccaat cctctagggt atctatatcc tgcacctcgt gggagggcac
      661 aataaaggcg gcagaattgg ggctaaatat acggcgtttt tccaaccaag ccgttgcact
      721 cccccaataa aattgccccg catcgtgaaa cgcgggcgtt aaatcctggg agcgagtgag
      781 ggcatattca gggtaaaaca tctctaccct gccctcggcc gttaaataaa tcgccctctg
      841 aatgggaaaa tcaaacgctg tggcagaaaa tacaaaatca cagcgctcgc gctcaaagac
      901 tgtacaggct tgttgcaatt gttctgcagt tacaaaggcc gcggttgcat ataaacaaca
      961 cacactctcc actggctgcc cgttggactg taaaaaactg attgcatggg cgattacatc
     1021 agcggtaatg gcgtggtcat cggcaatgtc tgccgggcgc acaaagggca cctgggcgcc
     1081 ggcgccaatt gccacttggg caatttctgc atcatccgtg gagcacaaca caagatcaaa
     1141 taattcactc tccagcgcag cttttatcgc ataatgaata ataggctggc ctaaaaacgg
     1201 ttttatattt ttgcgcggaa tgcgcttgct gccaccacgg gcgggaataa cggctacgcg
     1261 catagattgc gctccagttg ttctagtaca taatcctgtt gcgcaaaggt catctccaca
     1321 aataacggca aagttaaggc accggcataa taagcttccg ctagcggata atcccccgct
     1381 ttaaagccta acgcttggta aaacggctgc agatgaatgg gaatataatg cacgtttaca
     1441 cctatgccag aggacttaag cgcattaaat acagttaagc gcagttgagg atcaacaccc
     1501 accgcataaa tatgatgcgc cacgcgctgc cggctgggca catggggcag cgcaataggc
     1561 aattttgcca acagcgtttg gtagcgctca aatagctgcc tgcgcttgtc aataaaggtt
     1621 tccagtttgc ccaattgcac tacgcccaca gcggcttgaa tatcacacaa gcgataatta
     1681 aaaccgaggc tttgttgttc gtaataccaa ggtggtggat taggatcggt aaattcctgg
     1741 ggatttttgg taataccgtg ggtaacaaaa cgccgcagcc gattggcaat cacactatcg
     1801 gctgtagtaa tagcaccacc ttcaccggtg gtgaggtttt ttaccggatg aaaactaaaa
     1861 caggtggcat gactaaaacg acaggcgccc acaggcacat tatttacctg ggttcctatc
     1921 gcatgggcgg catcttctac tacataaaaa ccatactgct gcgccaatgc aaaaattgcc
     1981 ggcatatcac aggcgacacc ggcaaaatgt accgccacga caattttcgg cacccgcccc
     2041 ttcgccaatt tagcggctaa ttgttgcggg cacatattgc ggctgacggg atctatatcg
     2101 acaaaatcaa cttttgcccc caaatacaaa ccacaattag cggaagctac aaatgaatta
     2161 ggcgatgtcc ataattcgtc gccaggcccc aggcctaaag ctgcacaggc ggcgtgtaag
     2221 gctgcggtgc cattggagca ggtcactgcg tggggcgcct gtacatatgc ggccagggcc
     2281 tgttcaaact caacaacctt aggcccttgg gttaaccaat ctgactgcaa tacctcccgc
     2341 gcagcgtcaa tttccaattg ggaaatatgt tgacgtccgt agggaatcat acctcggcca
     2401 tctcatgcag ctggcgaatt tcatccacgt ttaacacatg ggggttattg ccggaattgt
     2461 acgaaaaccc ccgcggcacc gtgtggcccc gctcacctaa attattaatt tcataattca
     2521 cgtagcggaa taatttaatg gtgggggtta tcacaaaatg gtcgtcaaat tccaaggtta
     2581 gatgggaatc atccagcggg cacatggttt catgcagttt ttcaccgggg cgaatgccga
     2641 caattttggt tggcatttca ggcgctatgg cttcggccaa a
//
LOCUS       DAOQGS010000571         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23794_length_2678_cov_4.012200, whole genome shotgun sequence.
ACCESSION   DAOQGS010000571 DAOQGS010000000
VERSION     DAOQGS010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23794_length_2678_cov_4.012200"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKZ52_16345"
     CDS             complement(<1..808)
                     /locus_tag="PKZ52_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNG61394.1"
                     /translation="MFEFAWPWLLLALPLPWLAYRFLPPRPSAQAALLVPFFERAQDL
                     LNQTSGAKQQAWLRRLLALALWVCLVLAAARPQWVGDTISLPSSGRDLLMAVDISGSM
                     QIEDMPVNGTPISRIAVIKNVVGDFVVRRQNDRLGLVLFGTQAYLQAPLTFDRQTVNK
                     FLQEAQLGFAGEQTAIGDAIGLSIKRLKNRPAQSRVIILLTDGANTAGELEPRKAADL
                     AKQAGVKIYTVGIGANEMIEPGFFGLSRKVNPSADLDEGTLTYIADTTGGK"
     gene            complement(801..1550)
                     /locus_tag="PKZ52_16350"
     CDS             complement(801..1550)
                     /locus_tag="PKZ52_16350"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HNG61395.1"
                     /translation="MADSSNAFSSAANSSSAASSSASSAANSPLTSPAPGGNQSVTNL
                     QQQLQPAHQDPVNHPAFGAPPEQEMQSLIAQISDIERPAEIGIWPLAPGWWVLFALLA
                     VGLILGAIALYKTIKRRAYRRSALRMLKRLGDGSNLSRSGLARQLNEILKRTALASPY
                     YRHLYIGSAHGEAWREFLCASSPEFPDKAKTVDNLLQSVYRPKAQLDAGVSIAFCEFW
                     IRQHHHMVEPEQTSGASRVINIEKTGEPRHV"
     gene            complement(1540..2508)
                     /locus_tag="PKZ52_16355"
     CDS             complement(1540..2508)
                     /locus_tag="PKZ52_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNG61396.1"
                     /translation="MRAGHTGVYLLPEDLILLRGKAAQLNLHRQAPSRAAMAGTSHTR
                     FRGRGMEFAEVRPYQAGDDIRTIDWRVTARTQVPHTKLFQEERERPVFVLVDQRSPMF
                     FGSQHCFKSVYAAELAALIAWACHQGGDRLGGVLLGDHAQQDLRATRGKPGLLHFLNA
                     LVEYNQRLFSPVPKAKALPLLPALEQLGQVVKPGSQVFLLSDFHDLDNACQESLSRMA
                     KHSDVIALHIFDPLEADIPQGSLLRISNGFKRLLLSGSDLKAPFNASFRSQQQQVQTV
                     CGASRVVFASCPISADSLSPLRRLFAEGSHKGAAALNSLAGALYGR"
     gene            complement(2511..>2678)
                     /locus_tag="PKZ52_16360"
     CDS             complement(2511..>2678)
                     /locus_tag="PKZ52_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61397.1"
                     /translation="AHAWLKGKDFVSPEDVQAVVHDVYRHRLLLSFEAEARGQTADSV
                     IHTILQLVPVL"
BASE COUNT          536 a          766 c          861 g          515 t
ORIGIN      
        1 acttgccgcc ggtggtatcg gcaatgtagg taagggtgcc ttcgtctagg tctgcggagg
       61 ggttaacctt acgggacaga ccaaaaaagc ccggctcaat catttcattg gcacctatgc
      121 cgacggtata gatcttcacc cccgcctgct tggcgaggtc ggcggctttt ctcggttcca
      181 attccccagc ggtgttggcg ccgtcggtga gcaggataat cacccggctc tgggcggggc
      241 ggtttttcag ccgtttgatg gacaggccga tggcgtcgcc tatggcggtc tgttcaccgg
      301 caaagcccag ctgagcctcc tgcaaaaatt tgttcaccgt ctgccgatca aaggtcaagg
      361 gcgcctgcaa gtaggcttgg gtgccgaaca acaccaggcc taagcggtcg ttttggcggc
      421 gcaccacaaa gtcgccgacg acgtttttaa tcaccgcaat acgggagatg ggcgtgccgt
      481 tcaccggcat gtcttcaatt tgcatactgc cggagatgtc caccgccatt aacagatccc
      541 gcccgctgct gggcaggctg atggtgtcgc ctacccattg ggggcgagcg gcggccagca
      601 ccaggcacac ccaaagggcg agggcgagca ggcggcgcag ccaggcctgt tgtttggcgc
      661 cgctggtttg gttgagcaga tcctgggcgc gctcaaaaaa cggcactaac agcgccgcct
      721 gggcggaggg gcgcggcggc aaaaagcggt aggcgagcca gggtaagggc agggcgagca
      781 gcagccaggg ccaagcaaat tcaaacatga cgcggctccc ccgttttttc gatgttaatc
      841 acccggctgg cccctgaggt ctgctccggt tcaaccatgt ggtgatgctg gcgaatccaa
      901 aactcgcaaa acgcgatgct cacccccgca tccaactggg ccttgggccg gtagacggac
      961 tgcaacaggt tatccacagt tttggctttg tcgggaaatt ccgggctgct ggcgcagagg
     1021 aattcccgcc aggcctcgcc gtgggcgctg ccgatataca agtggcgata atagggggag
     1081 gccagcgccg tgcgcttgag aatttcattc agctggcggg ccaggcccga gcgcgacagg
     1141 ttactgccgt cgcctaagcg tttgagcatg cgcagggccg agcgtcggta ggcgcggcgc
     1201 ttgatggttt tgtacagggc aatggcgccg aggatgaggc ccaccgctaa cagggcaaac
     1261 aacacccacc agccgggggc caggggccag atgccgattt cggcggggcg ctctatatcg
     1321 ctaatctgcg cgatgaggct ctgcatctcc tgctcaggcg gtgccccaaa agccgggtgg
     1381 ttgaccgggt cctgatgggc gggctgcagc tgttgctgca ggttggtaac cgactgattg
     1441 ccgccggggg cgggcgaggt cagtggggaa ttggcagcac tgctggctga actgcttgcc
     1501 gctgagctgc tattggcagc gctggaaaaa gcgttggaac tatcggccat agagtgcccc
     1561 cgccagtgag ttcagtgctg ccgccccctt gtggctgcct tcggcaaata ggcgccgcag
     1621 gggcgagagg ctatcggcgg aaatcgggca gctggcaaac accactcggc ttgcgccaca
     1681 gacggtttgc acctgttgct gttggctgcg aaagctggcg ttaaacggag cttttaaatc
     1741 gctgccggat aacaacaaac gcttaaaacc gttgctgatg cgcagcaggg agccctgggg
     1801 aatatctgct tctaaggggt caaaaatatg cagggcgatg acatcgctgt gcttggccat
     1861 gcggcttagg ctctcctgac aggcattgtc taaatcgtga aagtccgaca gcaaaaacac
     1921 ttggctgccg ggtttaacca cctggcccaa ctgctccaag gcgggcagta ggggcaaggc
     1981 tttggccttg ggcaccggcg agaacaagcg ctggttgtac tccaccaagg cgtttaaaaa
     2041 gtgcaataaa ccgggtttgc cacgggtggc gcgcaggtcc tgttgggcgt gatcccccag
     2101 cagcacgccg cccaagcgat cgcccccctg gtggcaggcc caggcgatca gggcggcaag
     2161 ttctgcggca tagacggatt taaaacagtg ttggctgcca aaaaacatcg gcgagcgctg
     2221 atccaccagc acaaacaccg gccgctcccg ttcttcttgg aacagcttag tgtggggcac
     2281 ctgggtgcgg gcggtgacgc gccagtcgat ggtgcgaata tcatcccccg cctgataggg
     2341 gcgcacctcg gcaaactcca tgccccggcc gcgaaaacgc gtgtggctgg tgcccgccat
     2401 ggcggcgcgg ctgggggcct ggcgatgtaa atttaattgc gccgctttac cccgcaggag
     2461 gataaggtcc tcagggagca gataaacgcc ggtatgacct gctctcatgc ttatagcacc
     2521 ggtaccagct gcaaaatggt gtgaatcaca ctgtcggcgg tttggccgcg ggcttcggct
     2581 tcaaaactca acagcaggcg gtggcggtac acatcgtgaa ccaccgcttg tacatcttcc
     2641 gggctgacaa aatctttgcc cttcagccaa gcgtgggc
//
LOCUS       DAOQGS010000572         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23831_length_2676_cov_4.489508, whole genome shotgun sequence.
ACCESSION   DAOQGS010000572 DAOQGS010000000
VERSION     DAOQGS010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23831_length_2676_cov_4.489508"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..1704
                     /locus_tag="PKZ52_16365"
     CDS             379..1704
                     /locus_tag="PKZ52_16365"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNG61398.1"
                     /translation="MAIKTQMLTRCGVPILLSLLACACSGPVDTASSSSSSSSSSSSS
                     SSSSSSSSSSSGRTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGINLPLVVAINAGS
                     SNTATFEGVTYKADQYSTGGSAHEVTNDIVGVTEDTLFQSERYGTYGYKIPVTAGTYT
                     IKLHLAELYWQAAGDRTFNVTVEGQVKLANLDLITQAGRFGAYTVVIPNVRVTDGALD
                     IAVDPSKDAGTMSGFAVYSADGKLDTSTPPVNTNCKAYVGITYDDGPVNTTAFVNALK
                     SAGLVPVTFFVNGTNIGSRPGAIKEMLTVGQVQSHAYTHVDMGSYSAAQVRSQLEQNN
                     TAIVNAGAPKPTIFRPPYGTVNSTIRQVASELGMITITWDNDSQDWNGAATSAIVSAN
                     DRLQDGQVILMHENQTNSLSAIPQIAAKLKAKGFCPGKLDPKTGRAVAP"
     gene            2347..>2676
                     /locus_tag="PKZ52_16370"
     CDS             2347..>2676
                     /locus_tag="PKZ52_16370"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485893.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNG61399.1"
                     /translation="MPTRYQLANAIRVLSMDAVQKANSGHPGAPMGMADIAEVLWNDF
                     LKHNPNHPEWPDRDRFVLSNGHGSMLIYSLLHLSGYDLAIEDLKQFRQLHSRTPGHPE
                     YGYTPGVE"
BASE COUNT          632 a          752 c          642 g          650 t
ORIGIN      
        1 atttattttt cacagtgttg gcaggtgtct cggcggtaac ctttgtggcg gcacagtttt
       61 taaacgagcc caaaggccat atggcggaag tgttagaaga cggcacagtg caattaattg
      121 aagttgaata aaccgcctta ttgtaaaaac cactgcgctt gcagtggttt tttttgtgcg
      181 gagtttgttg ctcgccgttc ccatggaagg tgttttgtta ctgtgtgatc accgttcaca
      241 gacaacaagt accttgcttc gcaaaaatct tacttattgt cgcaacgaat accccgattt
      301 tgaagcgcag cagagtttaa tattgcccgc tacttataat ccgtctttga aaacaacacg
      361 ctataaggcg gatttactat ggctatcaaa acccaaatgc tcacccgttg tggtgtgcct
      421 attcttttgt cactcttggc ttgtgcctgt agcggccctg tggatacggc ttcttccagt
      481 agctcatctt catccagctc gtcctccagc agctcatcat ccagcagttc aagctccagc
      541 tctggccgca ccagcagttc atcttccagc tcctccagca gctcttctag ctcttcttcg
      601 agcagctctt cctctagttc cagctcttcc agttccagta gcggaatcaa tttgcctttg
      661 gtggtcgcca ttaacgcagg ctccagcaat acggcaacct ttgaaggcgt aacctataag
      721 gccgatcaat attcaaccgg cggcagcgcc catgaggtga caaacgatat agtcggcgtg
      781 acggaagata ctctgttcca gtccgagcgt tacggcacct acggctacaa aatcccagtg
      841 accgctggca cctacaccat caagctgcac ctggcggaac tctactggca ggcggcgggg
      901 gatcgcactt ttaacgtaac ggtggaaggg caggtcaaac tggctaatct cgacctgatc
      961 actcaagcgg gccgcttcgg agcttatacc gtggtgatac ccaatgtgcg cgtaaccgac
     1021 ggcgctttgg atattgcggt ggatccatcc aaagatgcgg gcaccatgag tggttttgcg
     1081 gtttattccg ccgacggcaa gttggatacc tcgacaccac cggtcaacac caactgtaag
     1141 gcctatgtgg gtattaccta cgacgacggc ccagtgaaca ccaccgcctt tgtaaatgcg
     1201 cttaaatctg caggcctggt gccggtaacc ttctttgtta atggcaccaa catcggcagc
     1261 cgcccgggcg ccatcaaaga aatgctcacc gtaggccagg tgcagagcca cgcctacacc
     1321 catgtggaca tgggcagcta cagcgcagct caggtgcgct cgcagctgga gcaaaacaac
     1381 accgcgatcg tcaatgccgg tgcgcctaaa cccacgattt tccgtccacc ttatggcaca
     1441 gtaaatagca ccatccgcca agtggccagc gagctgggca tgatcaccat cacttgggat
     1501 aacgattcac aagactggaa cggtgctgcc accagcgcca ttgtttctgc taatgatcgc
     1561 ttgcaagatg gccaggttat tctgatgcac gaaaaccaaa ccaactccct ctcggcgatt
     1621 ccgcaaattg ccgccaaact caaagcaaaa ggattttgcc ccggcaaact cgacccgaaa
     1681 acgggccgcg cggttgcgcc ttaatttata agctggccgc tgtgcaggct taattaaacc
     1741 tgcacagaat ggcaattttt aacgcgcact aggctcgctt agtgcgcgtt tttgtttgcg
     1801 ggtgtggatc tctgcgcttt cgattgcggt ttttaggttt aactactcaa gtcagcttga
     1861 ttactctcac atcgcataat ccattttcgg tgcagcaatg gtgaatattg agggttcaca
     1921 gtaaaagctc tcgtctgatg gtttccaggc agcgcgggaa ttacatgccg ttacactcaa
     1981 cagctgctga gattggcact gtcgtttcat tgaaatattc agtccggaat caacagcgct
     2041 ccgacgcacg gtttgcgaac agaagttatg ttcaagctag ggttttggtt gaatttgcct
     2101 gacgcgagac ctaaacaaga attgtgtaaa tttatcgatc ggcgcgagcg ccgctaaacc
     2161 cagtgcaaca tagtcaattc gacacaaaaa aggcagctac tacgcttgcg gcagggggct
     2221 taatttaact gtgcattgag tggattaacc cttggtttct ccgccaaatc tgaaggataa
     2281 tgccgcgctt tgcctcgccc ggccataggc tggggccaat tcaccgctca actctgggag
     2341 tctctcatgc ccacccgtta ccaactcgct aacgcgatcc gcgtcctcag tatggacgcc
     2401 gtgcaaaaag ctaattccgg ccaccccggt gcccccatgg ggatggcgga catcgccgaa
     2461 gtgctctgga acgacttctt aaaacacaac ccgaatcacc ccgagtggcc cgaccgcgac
     2521 cgttttgtgc tctccaacgg ccacggctcc atgctgattt attccctgct gcacttaagt
     2581 ggctacgatt tagccattga agacctcaag caattccgcc aattacactc ccgaaccccc
     2641 ggccaccctg aatacggcta tacccccggc gttgaa
//
LOCUS       DAOQGS010000573         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23832_length_2676_cov_4.411675, whole genome shotgun sequence.
ACCESSION   DAOQGS010000573 DAOQGS010000000
VERSION     DAOQGS010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23832_length_2676_cov_4.411675"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..714
                     /locus_tag="PKZ52_16375"
     CDS             4..714
                     /locus_tag="PKZ52_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HNG61400.1"
                     /translation="MPGGSHWSHVFRRGTRLRITDPTGGASLALLMYNPISLGEKYNA
                     PDTLKCQHTFKLTQGHCLYSDMGRIFCSITEDSFGWHDTISGSSHKSDVAARWGERDY
                     QTQRNDWLQNGQDSFLVELAKYGLGLRDLAANVNFFSEVRADEEGQLRLCRQSQAGDY
                     VCLRFEMDTLVVMHSCPHRLNTAAAYPKLGLNLSLDLAPPITSDDLCLNHCPENRRGF
                     DNNALYHFGLPFAGLVSQ"
     gene            722..1366
                     /locus_tag="PKZ52_16380"
     CDS             722..1366
                     /locus_tag="PKZ52_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HNG61401.1"
                     /translation="MTIQTSSRDAAKAQQSLIVRAGDYYLGTVKAGQVLRIVDLEGNQ
                     AADTLFYNAADISERYSATDTIAAQGNLYLTAGTMLKSNLLRDMLTIVADTCGRHDTI
                     GGACATESNTVRYALEKRCMHACRDSWLLALQEHEDCGLTKADITHNVNFFMNVPVSP
                     EGGLKFADGISAPGKYVELLAHMDVLVLISNCPQLNNPCNAYNPTPIEVLIWEQ"
     gene            1580..>2676
                     /locus_tag="PKZ52_16385"
     CDS             1580..>2676
                     /locus_tag="PKZ52_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNG61402.1"
                     /translation="MFDTVLIANRGAIATRILRTLKHMGIKSVAVYHEEDANSLHVQQ
                     ADLAVCLGAGSAADTYLNIDKILKAAHDTGAQAIHPGYGFLSENQAFVQACEAAGLVF
                     IGPTLEQMALFGLKHSAREAAAAAQVPLVPGSPLLEDLTQAQRWAEQVGYPVMLKSTA
                     GGGGIGMQVCQDEVELTQAWDSVRRLGGNYFSNNGVFLEAYIARARHIEVQIFGDGQG
                     LAKALGERDCSAQRRNQKVLEETPAPNLPEDQRQALHTTAEALMASVQYRNAGTVEFI
                     YDVDRGAFYFLEVNTRLQVEHGVTEQVWGVDLVNWMIRQAAGELDLALEHGRLTPQGH
                     AVQARIYAEGLKNGRIRKSLSNLSGRSVKGG"
BASE COUNT          586 a          830 c          770 g          490 t
ORIGIN      
        1 tttttgcccg gcggcagcca ctggtcccac gtctttcggc gcggcacccg cctgcgcatc
       61 accgacccaa ccggtggcgc aagcctcgcc ctgctgatgt acaaccccat tagcctgggc
      121 gaaaagtaca acgccccgga caccctcaag tgccagcaca cctttaagct cacccagggc
      181 cactgtttgt attcggatat gggccgcatt ttttgctcca ttactgagga tagcttcggc
      241 tggcacgaca ccatctccgg cagcagccac aaaagcgatg tggccgcgcg ctggggggag
      301 cgggactatc aaacccagcg caacgattgg ctgcaaaacg gccaggacag ttttttagtg
      361 gagctggcca aatacggcct gggccttagg gacttggccg ccaacgtaaa ttttttcagc
      421 gaagtgcggg cggatgaaga gggccagctg cgcctgtgcc gccagagcca ggcgggggac
      481 tatgtgtgcc tgcgttttga gatggacacc ttagtggtga tgcacagctg cccccaccgg
      541 cttaacactg ccgccgctta ccccaaattg ggccttaacc tcagcctgga cttggcgccg
      601 cccataacct cggacgacct gtgcctcaat cactgcccgg agaaccgccg gggttttgat
      661 aacaacgccc tctaccactt tggcctgccc ttcgcgggcc tcgtttctca ataggtgccc
      721 tatgaccatt caaaccagta gccgtgacgc ggccaaggcc caacagagcc tgattgtgcg
      781 ggccggggat tattacctag gcacggtaaa agcaggccag gtactgcgca ttgtggattt
      841 agagggcaac caggccgccg acaccctgtt ttacaacgcg gcggatatca gcgagcgcta
      901 cagcgccacg gacaccatcg ccgcccaggg caacctgtac ttaacggcgg gcacgatgct
      961 caaaagtaat ttgctgcggg atatgctgac catagtggcc gatacctgcg gccgccacga
     1021 caccatcggc ggcgcctgcg ccactgaatc caataccgtg cgctatgccc tggaaaaacg
     1081 ctgcatgcac gcctgccggg acagctggct gctggccctg caggagcatg aggactgcgg
     1141 cctcaccaag gcggacatca cccacaacgt caactttttt atgaacgtgc cggtaagccc
     1201 cgaaggcggc cttaagtttg ccgatggcat ctcagcgccg ggtaagtacg tggagttgct
     1261 ggcccatatg gacgtgctgg tgttgatctc caactgcccc cagctgaata acccctgcaa
     1321 tgcctataac cctacgccca ttgaagtgct gatctgggag cagtgagctt gcctgaatac
     1381 agtacaactc ggcgtatcga cgtaggttgg gcagtaatgc ccaacgctta tcgcccaaca
     1441 gcagagcgtc tcagtcaaaa cccatgagtg ttgggcatga ccgcccaacc tacgcaaaaa
     1501 accagcttgc gccccaggac gacctggggc cagtcgaata aatgcgggac ggcccgccca
     1561 aacacggaac actcctacca tgtttgacac agtactgatc gccaaccgag gcgccatcgc
     1621 cacccgtatc ctgcgcaccc tcaaacacat gggcatcaaa agcgtggccg tataccacga
     1681 ggaagacgcc aactccctgc acgtacagca ggcggaccta gccgtgtgcc taggcgcagg
     1741 cagcgccgcc gatacctact tgaacatcga caaaatcctt aaagcagccc acgacacagg
     1801 cgcccaagcc attcacccag gctacgggtt cttaagtgaa aaccaagcct ttgtgcaggc
     1861 ctgcgaggcg gcgggcctgg tgtttatcgg gccgaccttg gagcaaatgg ccctgttcgg
     1921 tttgaagcac tcggcgcgag aagccgcagc cgccgcccag gtgcccctgg tgcccggctc
     1981 cccgctgctg gaggatttaa ctcaggccca gcgctgggcc gagcaagtgg gctacccggt
     2041 gatgcttaaa agcaccgcag gcggcggcgg tattgggatg caggtgtgcc aggatgaggt
     2101 ggaactcacc caagcctggg attcggtgcg ccgcttaggg ggcaattatt tttccaataa
     2161 cggcgtgttt ttagaggctt atatagcccg cgcccgccat atcgaagtgc agatttttgg
     2221 cgatggccag ggccttgcca aagccttagg agagcgggat tgctccgccc aacggcgcaa
     2281 ccaaaaagtg ctggaggaaa ccccggcccc caatctaccc gaggatcagc gccaggccct
     2341 gcacaccacc gccgaagccc tgatggcttc agtacagtac cgcaacgcag gtacggtgga
     2401 gtttatttac gacgtggacc ggggggcgtt ttatttcctc gaggtaaaca ctcgcctgca
     2461 ggtggagcac ggcgtaaccg agcaagtgtg gggcgtggac ctggtgaact ggatgatccg
     2521 ccaggccgca ggcgagcttg acctggccct ggaacacggc aggctcaccc cccaaggcca
     2581 tgcggtgcag gcgcgcatct atgccgaggg cttaaaaaat ggccgtatcc ggaagtctct
     2641 ctcaaacttg tccggcagaa gcgtcaaggg tggaat
//
LOCUS       DAOQGS010000574         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23849_length_2675_cov_4.425191, whole genome shotgun sequence.
ACCESSION   DAOQGS010000574 DAOQGS010000000
VERSION     DAOQGS010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23849_length_2675_cov_4.425191"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKZ52_16390"
     CDS             complement(<1..506)
                     /locus_tag="PKZ52_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61403.1"
                     /translation="MSARAFAEPYAHDRRHARHLQRQQLVLVAKPAKKAEIIPLVPRQ
                     FKQEKALELVYPKYTRLKIAALLGLVVGLHFVAFHYFHGKPAPEPIAKVATPPIKVER
                     YVPPEIPPQVIQPAPPKPQPKEPPKPVKPKPAKPPVERVNPNVIEERVAPPAPEKPGP
                     VQATGPSA"
     gene            1210..1470
                     /locus_tag="PKZ52_16395"
     CDS             1210..1470
                     /locus_tag="PKZ52_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61404.1"
                     /translation="MMKDDSWFMKDYGRIMQDEIMGAKFWSVSSCAQSQDPNALQLVV
                     RIARRETHCKRRKNRKINYGVLIFAVLGRIINCRGKWPDIKL"
     gene            complement(1494..>2675)
                     /locus_tag="PKZ52_16400"
     CDS             complement(1494..>2675)
                     /locus_tag="PKZ52_16400"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNG61405.1"
                     /translation="AERVNGKPLVWFDNAATTQKPQVVMDRLNYFYRHENSNIHRAAH
                     ELAARATDAYENARQTVANFLGAASADEIIFVRGATEAINLLANTFGERFIQPGDDII
                     VSQLEHHANIVPWHNLAERKGAKLKVIPVDDSGQLQISALRNLIGPRTKLIAVTQVSN
                     ALGTVTPIEEIIHIAHQAGVPVMVDGAQAVSHMQVNVQALDADFYVFSGHKVFGPTGI
                     GVLYGKSRWLEQLPHWQGGGNMIADVTFEKISYQPAPNKFEAGTGNIADAVGLTSALN
                     YVSSIGLAAINAYEHELLNYATAGLKTIPGLKLIGTATHKASVASFILDGYSTEQVGS
                     KLNEEGIAVRSGHHCAQPILRRMGVETCVRPSLAFYNTCEEIDRMLSVLNRLAGKNRL
                     Y"
BASE COUNT          635 a          558 c          747 g          735 t
ORIGIN      
        1 tccgccgagg ggccagtggc ttgcacaggg cccggttttt ctggcgcagg cggtgccacg
       61 cgctcttcaa ttacatttgg gttcacgcgt tcaaccgggg gttttgcggg cttgggtttt
      121 accggtttgg gcggctcttt cggttgtggt ttgggcggcg ccggttggat aacttgcggt
      181 ggtatttctg gtggtacata acgctcaacc ttaatgggtg gcgtggctac tttggcgatg
      241 ggttcggggg ctggtttgcc gtgaaaataa tgaaatgcaa caaaatgcaa gcccacaacc
      301 aggcccaata aggcggcaat tttaagccgt gtatatttcg ggtaaaccag ctctaatgct
      361 ttttcctgtt taaattggcg cggtacgagg gggataatct cggctttttt ggcaggcttg
      421 gctaccagta caagttgctg acgttgcagg tgccgcgcat ggcggcgatc gtgtgcgtag
      481 ggttccgcaa aggctctggc actcataaaa aaatccttcc cgcgcagcgg gggtttaact
      541 taaacgtggt gtatatgttt aaacaaaagc ggttgggagc acgcgtgggc atctcagggc
      601 tcgctgttat ctacaccagt ttttgcagct atcgtgccag gtgatatttt gatttttaat
      661 gctgttttta tgcggatttt gcctatatcg gcaaacttag ccggcgggca gcggttagct
      721 gctgttggat ttggcgcagt ggtcatgcct agatttaagc agggtgttaa aaatgtgcag
      781 tagatcgaaa aaataattgc ctttgcatca ggcggtggtg gaactttgca aaagtggcca
      841 gcagggggct ggtatcgatt ttaccggcgg ataagaaatt aaataaattg taactattca
      901 gctcatttct catttttccc gcgttcgcgg ggatgaaaaa ttgaattatg agctgcttaa
      961 gaagcagtta cgtgctaact aagggggttt ctcgttccca cgctctgcgt ggaaatgcat
     1021 agttgttggg aattcgattg catgaattaa gcagataggc attcccacgc agagcgtggg
     1081 aacaaagaaa acggatcctg cgactgcgcg caggatgacg gttaggtttt gaaggatgtt
     1141 ggttaggttt tgaaggatgt tggttaggtt ttgaaggatg ttggttaggt tctgaaggat
     1201 gttggttggg tgatgaagga tgatagttgg tttatgaagg attatggtcg gattatgcag
     1261 gatgagatta tgggggcaaa attttggagc gtttcatcct gcgcgcagtc gcaggatcca
     1321 aatgcactgc aactagtggt gcgaattgca aggcgtgaaa cacactgcaa gcgacgtaaa
     1381 aacagaaaaa ttaattatgg tgtactgata tttgctgttt tgggaagaat aattaattgc
     1441 aggggaaaat ggccggatat taaattatga gtttaatatc cggtaggagg aatttaatac
     1501 agacgatttt tgcccgctag gcgatttaga acactgagca tccggtcgat ttcttcgcag
     1561 gtgttataaa acgcaaggga agggcgcaca caggtttcta cccccatgcg gcgtaaaatc
     1621 ggttgtgcgc agtggtggcc ggagcgcacg gctatacctt cttcgttgag ttttgagccc
     1681 acctgttcgg tggagtagcc gtctaaaata aatgaggcca cactggcttt atgagtggcg
     1741 gtaccaatta attttaaacc cggaatagtc tttaagccgg ctgttgcgta gtttaataac
     1801 tcatgctcgt acgcatttat ggcggcaagg ccaatgctgc tgacatagtt aagtgcgctg
     1861 gttaagccta cggcatcggc aatattaccg gtgcccgcct caaatttatt gggcgctggc
     1921 tgatagctaa ttttctcaaa ggttacatcg gcaatcatat tgccgccgcc ttgccaatgg
     1981 ggcaattgct ctaaccagcg cgattttcca tacaacactc caatgccggt ggggccgaat
     2041 actttgtggc cggaaaatac ataaaaatcg gcatccaggg cctgcacatt cacctgcatg
     2101 tgggataccg cttgcgcgcc atccaccatc accggtacac ccgcctggtg tgcaatgtga
     2161 ataatttcct cgatgggggt aacggtgccg agggcattgg agacttgggt cacggcgatt
     2221 aattttgtgc gcgggccaat taaattgcgc agcgcactta tttgcagctg gccggaatca
     2281 tctaccggta ttacttttag cttggcacct ttacgctctg ctaaattatg ccagggcact
     2341 atgttcgcat ggtgttccaa ttgcgaaaca ataatatcat caccgggctg aataaagcgc
     2401 tcaccaaatg tattggctaa taaattgatg gcttccgttg cgccgcgcac aaaaataatt
     2461 tcatcggcac ttgccgcacc taaaaaattc gccacggttt ggcgagcgtt ttcataggcg
     2521 tctgtggccc gagcagctaa ctcatgggcc gcacgatgga tattagaatt ttcgtggcgg
     2581 taaaaataat tcagccggtc catcaccact tggggttttt gcgtggtggc ggcattatca
     2641 aaccagacta atggcttgcc attgacccgc tcagc
//
LOCUS       DAOQGS010000575         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23852_length_2675_cov_3.739313, whole genome shotgun sequence.
ACCESSION   DAOQGS010000575 DAOQGS010000000
VERSION     DAOQGS010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23852_length_2675_cov_3.739313"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKZ52_16405"
     CDS             <1..788
                     /locus_tag="PKZ52_16405"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNG61406.1"
                     /translation="LKPDPALQARIDALIQTIAAAQEPDGYIYTWRTIAERNQKNGTP
                     PGTEGTGARMASSQRWVNEDQHSHELYNAGHLYEAAVAWYYATGSRKLLDVAIKNADL
                     VDRTFGPGKLKKAPGHQEIEIGLAKLYQATNERRYLNLARFFLDARGYGEAYMQNHQP
                     LRDQREAVGHAVRASYMFAGAADVMAFSSDTVFMPALRAVWDDIVSSKMYVTGGVGST
                     GSNEGFSDPYELPNYSAYCETCASIAFALWNQRMFQLTGSIKG"
     gene            743..2098
                     /locus_tag="PKZ52_16410"
     CDS             743..2098
                     /locus_tag="PKZ52_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61407.1"
                     /translation="MEPTNVSTHRLNQGIGNRFFVRMINGEPVNDAEYVHTLLGYLPP
                     ALFHNFVVTEFKCSLPPCDKTQSTKHRRQMLRAAISQLDFHTRQQINTIAETIILLSD
                     DIGHTVLDGFAFDYCEPACYQFFTTLPDQYQRSLWVFTHHRRLFDEALHARQMDIVRQ
                     HVICYSGFTAPRHLSAVDTHESRHTLQREVAAHLGCPVSSVAVEMTQQVRPTDTPACT
                     ADIVHIRIYHNARRMLMDCVRKGERVSRRIIRAETTHIIYEPAKGHIEVGSTTAIHRD
                     FLAHLVLRVALQADESARQNSITEAKNDPATELTPIRFYDYQRLSTPCVFDLSEEPIA
                     HTKVIELGYCDAANRTFLMKINYLDAEDIYTASKSLINPLFDFSQYQLNYAKLAVRVR
                     HTNPAQERTISIVLRGDNECNIKSKHNQDRALCDRLLHKWHLIKDRKQRASSAHDFVA
                     A"
     gene            2064..>2675
                     /locus_tag="PKZ52_16415"
     CDS             2064..>2675
                     /locus_tag="PKZ52_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61408.1"
                     /translation="MLPPLTISSLLELTTLFEHSSVFTDHQGQHLKGVPRGRLKHSAL
                     SAAELADWSAIVGYTNTVAVRCEDRTLLLAIEHDQASNGWFYRSPETYRKCAVALDDL
                     AIIAISAAKLLHYLADLLAIPMALRTGIQSPAIPEVLWHLGKTRVQGMGIDIWCARHL
                     TTQATLVIQHLKRRILPDTGLVLTFGPDLPEIVAPPRQYRWVAL"
BASE COUNT          662 a          767 c          633 g          613 t
ORIGIN      
        1 aactaaaacc cgatcctgcc ctccaggccc gaatcgacgc gctcatccaa accattgcag
       61 cagcccagga acccgacggt tacatctaca cctggcgcac catcgccgaa cgcaatcaaa
      121 aaaacggaac gcctccgggc accgagggca ccggggctcg catggcttcc tcgcagcgct
      181 gggtcaacga agaccagcac agccacgaac tgtacaacgc aggacacctc tacgaagcgg
      241 ccgtggcctg gtactacgct accggcagcc gcaaactgct tgatgtggct attaaaaacg
      301 ccgacctggt ggaccgcact tttggtcccg gcaaattaaa aaaagcgccc ggtcaccagg
      361 aaatcgaaat cggactggcc aaactctacc aggccaccaa cgaacgccgc tacctcaatt
      421 tggcccgatt tttcctcgat gcccgaggct atggcgaagc ctatatgcaa aaccaccagc
      481 ccctgcgcga ccaacgcgaa gcagtagggc atgccgtgcg cgcatcctac atgttcgccg
      541 gcgccgccga tgtgatggct ttcagcagcg ataccgtatt tatgccggcc ttgcgcgccg
      601 tttgggacga tatcgtgagc agcaaaatgt acgttaccgg cggcgtgggt tccaccggca
      661 gcaacgaagg cttttcagac ccctacgagc ttcccaatta cagcgcctat tgcgaaacct
      721 gcgcctccat cgcttttgca ctgtggaacc aacgaatgtt tcaactcacc ggctcaatca
      781 agggataggg aatcgattct ttgtcaggat gatcaatgga gaacccgtga acgacgccga
      841 atatgtacac acgcttttag gctatttacc gcctgctctt tttcacaatt ttgtagtgac
      901 agaatttaaa tgctcactac ccccatgcga caaaacccaa tcaacgaaac atcggcgcca
      961 gatgttgcgt gcggccattt cacaactgga tttccacaca cgccagcaga tcaatacgat
     1021 tgccgaaacg attattttat tgtcggatga tatagggcat acggtgctgg atggcttcgc
     1081 gtttgattat tgtgaaccgg cttgttatca attctttacc accctgccgg atcaatatca
     1141 gcgatcactg tgggtattta ctcaccatcg ccgcttattt gacgaggcat tgcacgctcg
     1201 acaaatggat atcgtgcgcc aacacgtcat ttgttattca ggttttaccg cccctcgcca
     1261 tttatctgcg gtagataccc atgaatcgcg ccatacgctc cagcgtgagg tagccgccca
     1321 tttaggttgt cctgtatcga gtgttgccgt tgaaatgacg caacaggtac ggcctaccga
     1381 cacgcccgcc tgcacggctg atattgttca tattcgcatt taccacaatg cccgccggat
     1441 gctgatggat tgtgtgcgaa aaggtgaacg ggtatcgcga cggattatcc gtgccgaaac
     1501 gacgcatatt atctatgagc ctgccaaagg ccatattgag gtgggatcga ccacggcgat
     1561 tcatcgggat tttttagcgc acttggttct gcgtgtggcg ctgcaagccg atgaatccgc
     1621 gcggcaaaac tcgataacag aggcaaagaa cgaccccgcg acagaattaa cgcccattcg
     1681 attttatgac taccaacggt tatcaacacc ctgcgtattt gatctctcag aagagcctat
     1741 cgcccatacc aaagtgattg agttgggtta ttgcgacgcc gccaaccgca cctttttaat
     1801 gaaaattaat tatctggatg ccgaggatat ttataccgcc tccaaatcat taattaatcc
     1861 actattcgac tttagccaat atcaactaaa ttacgccaaa ttggcggtgc gggtacggca
     1921 tacgaacccc gcacaggagc gcaccatttc aattgttttg cgcggcgata atgagtgcaa
     1981 tatcaaatcc aaacacaacc aagatcgcgc gttgtgcgac cgcttactgc ataaatggca
     2041 tttaattaag gaccgaaaac agcgtgcttc ctccgctcac gatttcgtcg ctgcttgaat
     2101 taacaaccct ctttgaacac tcctccgtat tcaccgatca ccaaggccaa cacctaaaag
     2161 gtgtccccag agggcgcctc aaacactccg cattatccgc tgctgaatta gcagattggt
     2221 cggcgattgt tggctatacc aatacggtcg cggtccgctg tgaagatcgc acgcttctgc
     2281 tcgcgattga gcacgatcaa gcgtctaatg gatggttcta ccgctcaccc gagacctatc
     2341 gcaaatgcgc ggtggcactg gatgatctcg ctatcatcgc catctctgcc gcgaaattgt
     2401 tgcactatct ggcggatcta ttagcgatac ccatggcgtt gcgaaccggt attcagtcac
     2461 cagccattcc cgaggtgctc tggcatttgg gtaaaacccg tgtccagggg atggggatcg
     2521 atatttggtg tgcccgacac ctgaccacac aggccactct ggttattcag cacctgaaac
     2581 gcagaatact cccagataca ggattggtac tgacgtttgg cccagaccta ccggagattg
     2641 ttgcaccacc gcgtcaatat cggtgggtcg ccctt
//
LOCUS       DAOQGS010000576         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23899_length_2672_cov_4.405426, whole genome shotgun sequence.
ACCESSION   DAOQGS010000576 DAOQGS010000000
VERSION     DAOQGS010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23899_length_2672_cov_4.405426"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKZ52_16420"
     CDS             <1..360
                     /locus_tag="PKZ52_16420"
                     /inference="COORDINATES: protein motif:HMM:NF017849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNG61409.1"
                     /translation="AAAQSAKICGLWLSADDGLDSLPAGHKSIPVIAIYFPAFTDGRG
                     YSIARQIREQKNYEGELRAIGNFIQDQVFMMKRCGFDAYLVPDDADLESYRTTLGILT
                     DYYQAAVDEPRPLFRRR"
     gene            complement(439..861)
                     /locus_tag="PKZ52_16425"
     CDS             complement(439..861)
                     /locus_tag="PKZ52_16425"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNG61410.1"
                     /translation="MRKFLGFTLIEVLVTVAIVAILAAIAIPSYSQYLQKTRRTDAQE
                     KLLDMAAQQERWFFNKNQYTDDVADVGGATSKDGYYTVTISNLGSKPYTKYTLTATPV
                     TTKSQNNDSECTSFTIDNTGARHAFKGSTPKDDFCWPK"
     gene            complement(869..>2672)
                     /locus_tag="PKZ52_16430"
     CDS             complement(869..>2672)
                     /locus_tag="PKZ52_16430"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNG61411.1"
                     /translation="ATGFSVTSKNTTWLDFTKGSNTDLVNFIRGKSIANFRDRRIDFV
                     PGKSNDTDENNAADGVEPWLLGDIVNSSPLVVSSATAGYDTDYGDETYTKLRDKYKNR
                     RNVLYVGANDGMLHAFNLGFFNADTKKYDAGGAPIGAELWAYIPFNLLPHLKWLTETD
                     YHHNFYMDGRVKLYDVNIFTPDDDHPDGWGSILVATMRTGGTPYELTQTQANGDTVKS
                     TLRSAVVVMDITNPEVAPKLIAEIPMPDNTYTTVNPDIFKFREFNTDGTIALNKWYLA
                     LGSGVTDRSLFTSDKTPKIFVFDLINKKWVNNGTGVTVGTNKGWIGGINARDWDRDYY
                     DDYMYFGTVEGTPSTPSGRLYRAKLSSDGSVGTPTPVLEDTTQAFAATPFTVVDTRNK
                     YWVFAGTGRYFSDSDNLNRSSQNSYYAIKEQLAPSTNGIELNTTFSKSTLANLTGVDI
                     QEDDQLKSVVSVGTSTATTRKTLVKLVDQNNGWYFDFNSANSRNYTSTILSGSSLVFN
                     TYEPGDECTPYGTSRQYRRDFLSGLPHNFVKNSTDTKLLPAFRDLGIGAASDPTPGKV
                     PVSSTSLGAIDVEPSLISTSGGERQSWRELPYSQ"
BASE COUNT          692 a          602 c          621 g          757 t
ORIGIN      
        1 gctgccgctc aaagcgcaaa aatctgtggc ctgtggttat ctgcagacga tggcttagat
       61 tcactgcccg ctggtcataa aagcatcccc gtgattgcca tttatttccc cgcctttacc
      121 gatggtcgcg gctactccat cgcgcggcaa attcgcgagc agaaaaatta tgagggtgaa
      181 ttgcgggcaa ttggtaattt tattcaagat caggttttta tgatgaagcg ttgcggcttt
      241 gatgcctacc tggtgccgga tgatgcggat ttggaatcct atagaacaac tctaggcatt
      301 ctaaccgatt attatcaggc agcagtggac gagcccaggc ctctatttcg gcggcgttag
      361 tgctgaagcg ataatgcaat aaataataaa gcctcgaaat cgaggcttta ttatttatgt
      421 gcaattaaga tttgaatgct atttgggcca gcaaaaatcg tctttggggg tgctaccctt
      481 aaaagcgtgc cttgcaccgg tattgtcaat ggtaaaactc gtacactcgc tgtcattgtt
      541 ttgtgatttg gtggtaactg gtgtagcagt aagtgtgtac ttagtgtagg gcttggatcc
      601 tagattagag atggttaccg tgtaataacc gtcctttgac gtcgcacctc caacgtcagc
      661 aacatcatca gtgtattggt tcttattaaa aaaccaccgc tcctgctgtg ccgccatatc
      721 cagcaatttc tcctgggcgt ccgtacgccg agtcttctgc aaatattggc tgtagctagg
      781 gatcgcaatc gccgccagaa tagccactat ggctaccgtt accagcactt caattagagt
      841 aaaacccaaa aatttacgca taactaacct attgactgta gggcaactct cgccaggatt
      901 ggcgctcccc gccagatgtt gaaattaaag aaggttcgac atcgattgca ccaagcgacg
      961 tgcttgatac aggtactttc ccaggtgttg ggtctgatgc tgccccaatg cctaaatcac
     1021 gaaacgcagg taacaatttt gtgtcagtcg agttcttaac aaaattatgc ggtaaaccag
     1081 acagaaaatc acggcggtat tgccgcgagg ttccataggg tgtgcactca tctcctggct
     1141 cataggtgtt gaacactagg gagcttcctg aaagtatagt ggaggtatag ttgcgcgagt
     1201 tagcagaatt aaaatcaaaa taccagccat tattttgatc gactaatttg accagcgttt
     1261 tgcgtgtagt cgcagtgctt gtgccgacac taacaacgct cttgagttga tcatcctctt
     1321 gaatatctac tcccgttaaa ttagccagag tactcttgct aaacgttgtg ttcagctcaa
     1381 taccattggt agaaggagcc aattgttctt tgattgcgta atagctgttt tgactgctgc
     1441 gattaagatt gtcagaatcg gaaaaatacc tgcctgtccc cgcaaaaacc cagtatttgt
     1501 tccttgtgtc tacgacggta aaaggagtgg cggcgaaagc ttgagtagtg tcttctagca
     1561 cgggggtcgg ggtgcccaca ctgccgtcag aggacagttt ggctctatat agccggccac
     1621 ttggtgtgct tggcgttcct tctaccgttc cgaaatacat atagtcgtca tagtagtcgc
     1681 gatcccaatc gcgagcgttg attccaccaa tccaaccttt attggtgcca accgttacac
     1741 ctgtaccatt gtttacccat tttttattga tgagatcaaa cacgaagatc ttcggggttt
     1801 tgtcgctggt aaatagactg cgatcagtaa cgccagagcc tagagcgaga taccatttat
     1861 ttagtgctat agtgccatca gtattaaact cgcggaattt aaaaatgtca ggattaacgg
     1921 ttgtgtaggt gttatccggc ataggtattt ccgctattaa tttcggtgcc acctctggat
     1981 tggtaatatc catgacaact acggccgatc tcaaggtgga tttaactgtg tcaccgttgg
     2041 cctgggtctg cgttagctca taaggggtgc ctcccgtacg catggttgct actaaaatag
     2101 aaccccagcc gtcagggtgg tcatcgtcag gagtaaatat attgacgtca tataatttaa
     2161 ctcggccatc catataaaaa ttgtggtggt aatcagtctc agttagccac ttaagatgag
     2221 gtaaaagatt aaaaggaatg taggcccaca attcggcacc gataggcgcg ccacccgcat
     2281 catatttttt agtgtcggcg ttgaaaaaac ccaggttaaa cgcgtgcaac ataccatcat
     2341 tggcgcccac atataataca ttgcgacggt tcttatattt atcccgcagt ttagtgtagg
     2401 tctcatcgcc atagtctgtg tcatagcctg cggtggcaga gcttacaact agcggcgagg
     2461 aattaacaat atctccaagt agccaaggtt cgacaccatc tgctgcgtta ttttcatctg
     2521 tgtcgttact ttttccaggc acaaaatcga tacggcgatc tctaaaattg gctatagatt
     2581 ttcctcgaat aaagtttacc aaatcagtat tgctgccctt ggtaaaatct aaccacgtgg
     2641 tatttttgct tgtaacagaa aacccggtag cg
//
LOCUS       DAOQGS010000577         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_23915_length_2671_cov_5.099771, whole genome shotgun sequence.
ACCESSION   DAOQGS010000577 DAOQGS010000000
VERSION     DAOQGS010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23915_length_2671_cov_5.099771"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKZ52_16435"
     CDS             complement(<1..769)
                     /locus_tag="PKZ52_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNG61412.1"
                     /translation="MLSIFVVHQTTHETHEIHLNAEQLHAEAIYIGRQPGCAILLPDS
                     TVSRKHAQITLAGKEVRIVDLHSSGGTFYQGAALVPEEIVIIQEGADILIGPYQLRFN
                     YAAQQQDQTQFPDEGKTKVVVTKLPNFQPVALVKAEDWQYWDGQTIDVTCSKIFRETH
                     DVKTFIFTAAPNLLFRYKPGQFATLHLTINGKPVIRSYSISSTPSRPHYLSVTVKQVP
                     AVAPHPPGLVSNWLHSEFKVGQKIQISGPYGEFSCHLH"
     gene            complement(869..>2671)
                     /locus_tag="PKZ52_16440"
     CDS             complement(869..>2671)
                     /locus_tag="PKZ52_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG61413.1"
                     /translation="GGHFSIWRGGFTQFYEEPNKEGLAENLRKRLGQLRDDWSAYLAG
                     RSIKQLSADEVYMLGHLAPSLQNANKIAFYRDFLTDSVQQGQADSIEGRKVLAQLRES
                     CGVSEEEHIRIVDSLKGEYTLDDALLISANLRINSFKQALQQLIFTALEQGKSLSDAI
                     AQHAEAIGKLREDFALSSQEEAQVIEELGSASGALATAIKHLCAQLRDIQQLQLHLTQ
                     LASEVFIFTYLQHQAQHIVTQCVELFAAVNAQDENLTELGCEFAASHPQAAEWALTQT
                     QLELPQQLVNQLRTAIFVQDAQMAFRREIALLRLQKIDEPFVQLAVARTAQEYGYAWA
                     EAAFKQSLETLMPATNHTHMLPLKPVAMVKLRSIDGLEKIWEQGFSAGRADDNDWVMT
                     EQEASRHHFKIYQQQGVFYCEDLHSANGTILNGDFLQGQRQKLHDTNVISITHASAPL
                     YIDVLQRAARREYPAVEGFFLFAQLALGHTLSHQLLWQLCSQAHWYTDQLPAPTAEVL
                     AVVLKGQLQCVFSDTSGARENLLLDTGASIDLTQTPPLSTWVPSGGSFLCALWQKAQI
                     SQIIQLAPQFYVMLLEHSNTILQRLYQQARIHRH"
BASE COUNT          646 a          623 c          671 g          731 t
ORIGIN      
        1 gatgcaggtg acaggaaaat tcaccatagg gcccggaaat ttgaattttt tgcccgactt
       61 taaattcact gtgcaaccag ttggaaacca aacccggcgg gtgcggcgca acagctggca
      121 cctgtttaac tgtgacactt aaataatggg ggcggctggg ggttgagcta atggaataag
      181 agcgaatgac gggtttgccg ttaatggtta aatgcaatgt ggcaaattgg ccgggtttgt
      241 agcgaaataa cagattgggc gctgcggtaa atataaaggt tttaacatcg tgggtttcgc
      301 ggaaaatctt gctgcaggtt acgtcaattg tttgcccatc ccagtattgc cagtcctccg
      361 cttttaccag cgccaccggt tggaaattgg gcagttttgt taccaccact ttggtttttc
      421 cctcatcggg aaattgcgtt tgatcctgtt gctgtgccgc atagttaaac ctcagctggt
      481 aggggccaat taaaatatca gcaccttctt gaataattac gatttcctcg ggcaccaagg
      541 ctgcgccctg gtaaaaagta ccgccactgg agtgtaaatc aacaatacgg acctcttttc
      601 cagccagagt aatttgggcg tgtttgcgac tgacggtgga gtccggtagt aaaattgcgc
      661 agccgggttg gcggccaata taaatggcct ctgcatgcaa ttgttcggca ttcaaatgaa
      721 tttcgtgtgt ttcatgggtg gtttggtgga caacgaatat agacagcata cagttatttc
      781 caaaatcctc tgttaaacag agttgtgtgg attttcgtca ggcgcagata cttggccgcc
      841 cccactagac tggcatttac caggctgttt aatggcgatg gatcctcgcc tgttgatata
      901 acctttgcaa aatagtgttg ctgtgttcga gcaacattac ataaaactgc ggcgccaatt
      961 gaataatctg actgatttgc gccttttgcc aaagtgcaca taaaaagctg ccgcccgatg
     1021 gtacccaagt actaagcggc ggtgtttgcg ttaaatcgat actggcgcct gtatcgagta
     1081 ataaattctc ccgagcgccg ctggtatcgc taaatacaca ctgcaactgc ccttttaata
     1141 ctacagccag cacctcagcg gtgggggcgg gtaattgatc cgtataccag tgtgcctggc
     1201 tacacaactg ccacaataat tgatggctaa gtgtgtggcc gagggctaat tgggcaaata
     1261 gaaaaaaccc ttccaccgca ggatattctc gccgcgccgc gcgctgtagt acgtctatat
     1321 acagcggtgc gctggcatgt gtaattgaaa taacattggt atcgtgtaat ttctgccgtt
     1381 gaccttgtaa aaaatcacca ttgagaatag tgccattggc ggagtgtaaa tcctcgcaat
     1441 aaaacacacc ttgttgttgg taaattttaa agtgatggcg gctggcttct tgttcggtca
     1501 ttacccaatc attatcgtcc gcgcgtcctg cgctaaaacc ctgctcccag atcttctcca
     1561 ggccgtcaat gctgcgcagt tttaccattg ccacgggttt tagcggcagc atgtgcgtgt
     1621 ggttagttgc tggcattagt gtctccaaac tttgcttaaa tgccgcttcg gcccaggcgt
     1681 aaccgtattc ctgagctgtg cgtgccacag ctaattgcac aaagggttca tcaatttttt
     1741 gcaagcgcag cagggcaatt tcacggcgga acgccatctg ggcgtcctgt acaaaaatag
     1801 ccgtgcgcaa ctgatttact aattgttgcg gcagttcaag ctgagtttgt gtaagtgccc
     1861 attcggccgc ttggggatgg ctggcggcga attcgcagcc tagttcggta aggttttcat
     1921 cctgggcatt tactgcggcg aataattcta cacattgagt aacaatatgc tgggcctggt
     1981 gttgtaaata ggtaaatata aatacttcgg acgcaagttg tgttaagtgt aattgcagtt
     2041 gttgaatatc gcgcagctgc gcgcacagat gttttatagc ggtggccaaa gcgccactgg
     2101 cagagcccaa ctcttcaatc acctgagctt cttcttggct gctcagggca aaatcttcgc
     2161 gcagtttgcc aatcgcctcg gcgtgttggg caatggcgtc agacagactt ttgccctgtt
     2221 ctaaagcggt aaaaattaat tgttgcagcg cctgtttaaa gctattgatg cgcaagttgg
     2281 cagaaattaa cagcgcatca tcaagcgtgt attcaccttt tagggaatcg actatgcgta
     2341 tgtgttcctc ttcgctcacg ccacaactct cccgcaattg cgccagtact ttgcgccctt
     2401 cgatggaatc ggcctggcct tgttgtactg aatcggtgag aaaatcgcgg taaaaagcaa
     2461 ttttattggc gttttgtagg ctcggagcca aatggccgag catataaact tcgtcggcac
     2521 tgagttgttt aatactgcgc ccagctaaat aagctgacca atcgtcgcgc agttggccta
     2581 agcgtttacg caaattctcc gccaggccct ctttattagg ctcttcgtag aattgtgtaa
     2641 aacccccgcg ccagatggaa aaatggcctc c
//
LOCUS       DAOQGS010000578         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24045_length_2663_cov_5.077071, whole genome shotgun sequence.
ACCESSION   DAOQGS010000578 DAOQGS010000000
VERSION     DAOQGS010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24045_length_2663_cov_5.077071"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..833)
                     /locus_tag="PKZ52_16445"
     CDS             complement(417..833)
                     /locus_tag="PKZ52_16445"
                     /inference="COORDINATES: protein motif:HMM:NF015205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HNG61414.1"
                     /translation="MVTNNPNQSIEKQLVPVSMRATALMRHFPSSWFNVENQVFTVMS
                     QIAPDYCGGDWDFIELSNGAFFMAPTRPYKIVVPFGCYFECELGAEACGLLAMMFAYG
                     RLLEQTGDDAFLHHWSALGDFIAHHPDKLLLNRALD"
     gene            complement(941..1276)
                     /locus_tag="PKZ52_16450"
     CDS             complement(941..1276)
                     /locus_tag="PKZ52_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61415.1"
                     /translation="MQTVINGRFEFQDVDCAVEISSYRFSGRTALKVVAAQSKRNLFR
                     GLLEGTIIAIATINVPFPLSANEVIIFDEPPNTGILDALVNANIVTKTGRKITTQHGL
                     HEIVSINCN"
     gene            complement(1726..2313)
                     /locus_tag="PKZ52_16455"
     CDS             complement(1726..2313)
                     /locus_tag="PKZ52_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61416.1"
                     /translation="MKESSAILVVHYQVTHRDIDPPLVRFRCDHKSGFNLTKAQMLES
                     IKKSSLTIKYFNLIELVEVDDLDLVPTLKRLSQIPTERAMPAFTHGLEPLFSLHRQMG
                     VPIRYTVLLVFKWLQLDIASVCEEVGFHRNYLNAALSGKRQPSEVIRAKMVDKLGLDP
                     WGYAEWVLPTILDREIISICEHGDHAPRSSLAPLI"
     gene            2312..2530
                     /locus_tag="PKZ52_16460"
     CDS             2312..2530
                     /locus_tag="PKZ52_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61417.1"
                     /translation="MSLISSKNRLGCELDVDENCDIVVSQLLPNSKEQILVLTIEAAE
                     ELIINLKAAIEFAKDGGYAPNVYPHPHD"
BASE COUNT          773 a          628 c          579 g          683 t
ORIGIN      
        1 acagcattaa catggtgatt aaaatagctc ttttcttccc gattattacc ttgttgtctg
       61 ccaccaccct gctgcccata accattactg cggccatttt gctgctgacc ttggttgtta
      121 cggccatttt gagtgttttg acgattacgt gattgatatt gattgttcat ggtagtactc
      181 cgaaatataa aaaaaccgga tacaccatgc cccagcgggg atgtgtgttc cccggtgggt
      241 ttgaaggaat gccagatcat tcaagatccg tcgctaagcc aaaataggct gcggcatgga
      301 gcgacttgca gatcaacgag gaaacagccg ttgcactgtt agtaccgttt cattgtagat
      361 cattaactgg atgaaaaaaa aggccgctaa gcgacctttt aaaaaaaatt cctgaattaa
      421 tccagggcgc gatttaataa taatttatcg gggtgatggg ctataaaatc acctaaagca
      481 ctccaatgat gaaggaatgc atcatccccg gtttgctcca gtaggcggcc ataagcaaac
      541 atcatggcca acaagccaca cgcctctgcg cccagctcac actcgaaata gcagccaaat
      601 ggaacaacaa ttttataggg ccgtgtaggg gccataaaaa acgcaccatt cgacagctcg
      661 ataaaatccc aatcgccgcc gcaatagtcc ggtgcaatct gactcataac ggtgaacact
      721 tggttttcta cattaaacca gcttgaggga aaatgacgca taagtgccgt agcacgcatc
      781 gaaacaggca ccaattgttt ttcaatcgat tgatttgggt tattagtaac cataaggatt
      841 cctccaggaa gccccttcag aatcctagcc cactagggga aggatttccc ctggggcgag
      901 ttgataggtg cgaacaccca ttgttagtta gaaaccactc ctaattgcag ttgatcgata
      961 cgatctcatg caaaccatgt tgagtggtaa tttttcgacc tgtttttgta acgatatttg
     1021 cgttaaccag ggcatctaaa atgcctgtgt ttggaggctc atcaaaaatg atgacctcat
     1081 tcgccgataa cggaaacggg acattgatcg ttgcgatagc gatgatcgta ccctctaaca
     1141 ggccgcgaaa taaattgcgt tttgattgag ccgcaaccac ttttagtgct gttctaccgc
     1201 tgaatcggta cgaactgatt tcaaccgcac aatccacatc ttgaaactca aatcgaccat
     1261 tgatgaccgt ctgcatacat gacctcacga cagcgcatca agtgtactaa tgacttgcaa
     1321 caaggcatgg gccaaaggct ttatatcgtc tgcctccaat ttcaggttta caccttgagt
     1381 ccgaacaaca acctcgggtg agtgcgcggg ctttttggtc ataacaacag aaattttgtg
     1441 cgatctttta gctcggattg atttgagcac attgctaaaa aaagtagggc gttttgcgaa
     1501 taacataggt tatctccaaa ataaaaaaac cggagacacc tagccccatc gggataagta
     1561 atccccggtg ggttgagtga atgccgatcc caatgggatc cgtcactggg ccgataggcc
     1621 ttggcatgga gtgacgagcg gatcaacgag ataacagccg ttgcactgtg ttttacacgt
     1681 caattgtagc gattaatttt agtgagataa attaataatc tgtcactaaa tcaagggggc
     1741 caggctagac cgtggcgcgt gatcgccatg ctcacaaata ctgataattt ccctgtctaa
     1801 aatcgtaggc agcacccatt ccgcatagcc ccaaggatca agccccaatt tatcgaccat
     1861 tttggctcgg atgacctccg aaggctggcg cttaccactc agcgccgcgt tcaaataatt
     1921 gcggtgaaaa ccaacttcct cacacacaga tgcaatatcc agctgtagcc acttaaacac
     1981 cagtagcacc gtgtagcgga taggtactcc catttgacga tggagactaa acaatggctc
     2041 caaaccatgg gtaaatgcgg gcattgcccg ctcggtaggt atctgtgata atcgcttgag
     2101 agtgggcacg agatccaggt cgtccacctc gaccaactca atcagattga aatatttaat
     2161 cgttagagac gattttttaa tactctctag catttgcgct ttggtcaaat taaatcccga
     2221 tttgtgatca caacgaaaac gcaccaaagg tggatcgatg tctcggtgtg ttacttgata
     2281 atgcacaacc aaaatagcgg aggactcttt catgtcttta atttcctcaa aaaatagatt
     2341 gggttgcgaa ttagacgtcg atgagaactg cgacatcgtc gtttctcaac ttcttccaaa
     2401 cagtaaggag caaatacttg ttttaaccat tgaggcagca gaggaactta taataaattt
     2461 aaaggccgcc atcgaatttg caaaagacgg tggttacgca ccaaatgtct accctcatcc
     2521 acacgactga cccagagaaa aatagaacag gctacttaac ttcttttttt ccgccaaatg
     2581 gattttttaa ttggctcttg agcctgagcc gattcaaacc aaatgacacg gtgcaaatca
     2641 tcaacccgta aacgccatgc agg
//
LOCUS       DAOQGS010000579         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24298_length_2646_cov_4.244693, whole genome shotgun sequence.
ACCESSION   DAOQGS010000579 DAOQGS010000000
VERSION     DAOQGS010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24298_length_2646_cov_4.244693"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PKZ52_16465"
     CDS             complement(<1..1209)
                     /locus_tag="PKZ52_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNG61418.1"
                     /translation="MAVDLSQFRQVFIEESFEGLDSMEAALMALNINAIDSETINAIF
                     RAAHSIKGGSATFGYSAIAGFTHVLETLLDQVRSGQRSLDANSVNLLLKSVDAMRDML
                     ALLQTEQNCYTDSALALKAEFEALLQIPIAQSALAEKPLDRPEQKKPLNESVKAVEPI
                     LAGWKIEFLPGRDLFASGNCPVNILRELSQLGTVLIQPVLTAVPDIKNINPEECYLGW
                     QISLASPCQAEEIAAVFGWVEDQAVIRIEPVYDSARNDLPATHKNRYGGWHIHFKPKP
                     ELLHSGNDPLRIFRALQELGYCEISLGDNIELPDFHSYDSQLLYVHWQINCYGDLGYE
                     AIASAFDWVMGDCDLTITAIEPTEEIANETAEQPALTQVKKLAAALATSAPVEPMANN
                     VVPFITPAPAV"
     gene            complement(1439..1801)
                     /locus_tag="PKZ52_16470"
     CDS             complement(1439..1801)
                     /locus_tag="PKZ52_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG61419.1"
                     /translation="MAKILAVDDSASMRQMVTFTLKGAGHDVTEACDGVEALKVAKNQ
                     SKFDLVISDINMPNMDGIALIKELRSLPEFKFTPILMLTTESGTDKKSEGKAAGATGW
                     IVKPFNPDQLLATVGKVL"
     gene            complement(1820..1990)
                     /locus_tag="PKZ52_16475"
     CDS             complement(1820..1990)
                     /locus_tag="PKZ52_16475"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNG61420.1"
                     /translation="MHAGGVTRADTAGIQLLYALVLAARERQIALTWQNPSEKLRTAA
                     QILGMTSHLGIH"
BASE COUNT          653 a          642 c          645 g          706 t
ORIGIN      
        1 aacagcggga gctggcgtta taaacggcac cacattattg gccatgggtt ctacaggggc
       61 gcttgtggca agtgccgccg cgagcttttt cacctgtgtc agtgcgggtt gttcagccgt
      121 ttcattggct atttcttccg tcggttcgat ggcggtaatc gttaaatcac aatcccccat
      181 cacccaatca aaggcactgg caatcgcttc ataacctaaa tcgccataac agttaatttg
      241 ccagtgcaca tacagcaatt ggctgtcata ggagtgaaaa tcgggtaact ctatgttgtc
      301 cccaaggcta atttcgcaat agcccaattc ctgtaaggcc cggaagatac gcagcggatc
      361 attgcctgaa tgcagcaatt caggttttgg tttgaagtga atatgccagc ctccgtagcg
      421 gtttttatgg gttgcgggca ggtcgttacg ggcgctgtcg tacacaggtt ctatgcggat
      481 gacggcttgg tcttccaccc agccaaatac agcggctatt tcttcagcct ggcagggcga
      541 tgctaaagaa atctgccagc caagataaca ttcttctggg ttaatatttt taatatcggg
      601 aacagccgtt agcaccggct ggataagcac cgtgcccaat tggctaagtt cccgcaaaat
      661 attgaccggg caattgccgc tggcaaatag atctcgcccc ggcaaaaact ctattttcca
      721 tccggcgagt ataggctcaa cggctttgac tgattcgttt aaaggttttt tttgttcagg
      781 cctatccagc ggtttttctg caagggcgga ttgagcgata gggatttgca ggagcgcttc
      841 aaattcagct tttagtgcca aggcagagtc ggtataacag ttttgctcgg tttgcagtaa
      901 ggcgagcata tcgcgcatgg catctacaga ttttaacaac aggtttacgc tgttggcatc
      961 taagctgcgc tggccgctgc gcacttgatc gagcagggtt tccagcacat gggtaaaacc
     1021 tgcaatggcg ctatagccaa aggttgcact gccgcctttt atggagtggg cagcgcggaa
     1081 aatggcatta atggtttcgc tatcaatggc atttatattt aatgccatta aggctgcttc
     1141 catgctatca aggccctcaa aactctcctc gataaacacc tggcgaaatt ggcttaaatc
     1201 gacagccatg gggcctcctg tttttaatcg ccaatgactt aagtacgctc tgatttattc
     1261 gcacatagcc gtttcattac tgcgccgcgc actcatgtat aaaaaaacca tgggcgcggc
     1321 agccgcacat ggcacctatt tgcgggccgc atctttaaaa atgtgctgat taagcctgtg
     1381 gttgtgaata ttcaggtgcc tgcccgccgc atcgcaccca gacttaatca gaaattcctt
     1441 aaagcacttt gcccacagtt gccaataact gatccgggtt aaaaggcttt acaatccaac
     1501 cagtggcacc ggcggcttta ccttcggatt ttttatccgt accggattcg gtggttaaca
     1561 ttaaaatggg cgtgaattta aactccggca gggagcgcag ctcttttatc agcgcaatac
     1621 catccatatt gggcatattg atatcggaaa tgaccaaatc aaatttactt tgatttttgg
     1681 ccactttaag cgcttccacc ccatcacagg cctcggtcac atcatggcct gcacctttaa
     1741 gcgtaaaggt caccatttgg cgcatggatg ctgaatcgtc aacggcaaga attttagcca
     1801 tgtgttatct cctgattaat taatggatac ccaggtgcga cgtcataccc aatatctggg
     1861 cggcggtacg caacttttcc gatgggtttt gccaggtgag ggcaatttgc cgctcccgcg
     1921 cagctaacac caatgcatac aacagctgaa tgcccgccgt atcggcgcgc gtaacaccac
     1981 ctgcgtgcaa aataatgtga tcgttttttt atcgtcgacc agcgtttcca gctgttcgtg
     2041 aatggcttgt acatagccca ccgtgagctg ttcgggtaat tcaaaatcca atgcgtgcgc
     2101 catagtaatc ctcacatgtc taacgcttaa gctgcgcggg acaagccgct ggggtattta
     2161 gtgttaagga tagcccttga tttaaagtct gcattcttcg cgcggccttt gatttttcac
     2221 agcaaacaaa agcgaataag cctgtgagtt ttataatgat ggagcacaaa cgggcgttta
     2281 aaattgcaac taattagcac accttattgc cctccccaca cctatgtcat taagttaaga
     2341 aattaggccg caatttggat tgagaaatat ctgggtagcc tggatggagc gcagcgcaat
     2401 ccgggatttt cgatgcatcc cggattgcgc tgcgctccat ccaggctaca aaattggctg
     2461 acaagtctta acctaatgac gttgctctcc aggcgggaga aatgtttagg gggatttatt
     2521 aagccggcgg ctttttatgc cgggttaata gccaaatggc agcctcgatt cgcggtgtag
     2581 caggggtgaa gacaggttag tgcagaccgt caaaaagagg gacctttttg acgagcctac
     2641 acggat
//
LOCUS       DAOQGS010000580         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24455_length_2635_cov_4.041085, whole genome shotgun sequence.
ACCESSION   DAOQGS010000580 DAOQGS010000000
VERSION     DAOQGS010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24455_length_2635_cov_4.041085"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            539..1186
                     /locus_tag="PKZ52_16480"
     CDS             539..1186
                     /locus_tag="PKZ52_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNG61421.1"
                     /translation="MAAREFRLMMNFYMTPGSCSTGIHILLEELELVFSAQVVNLLAG
                     DTQKPAFLAINPKATIPVLVRDDGTALTDFIAIAWWLAATYPQAKLLPEGLDKQAEVL
                     NVMNDIVSGIHMQGFTRIFTPERYMAREGDKSWVEAQGRSCVDKGLALIERRLQAKPG
                     YLFGRFGIADAALFYVEFWASRINLALPAACAEHLRLMQSRPVVKRVLAEEGYRI"
     gene            complement(1295..1762)
                     /locus_tag="PKZ52_16485"
     CDS             complement(1295..1762)
                     /locus_tag="PKZ52_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNG61422.1"
                     /translation="MAKFLIVVGTVNGHAFAAAQGAAALLKKLGHSTQINVETQPADL
                     LRDADEIILACCSTTDQGKLPYNIYPLYRALEDQQLDLSNRHYGVIALGDSYFPPSQY
                     ALGGISLENAFYSCGAKRIGDIGLLDAQSVDNYPLAAALWVQNWLQKIPAINV"
     gene            1878..2222
                     /locus_tag="PKZ52_16490"
     CDS             1878..2222
                     /locus_tag="PKZ52_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNG61423.1"
                     /translation="MKYSEVIPYILSFDQVTRRTISESGTAIVFCLQERIFAYFETGA
                     PIQWKLSLEVSEAQLRELIYPPQITHAKDKPAGHWITIARVENCDEELLKELIAGSYA
                     RAQAKNATPVGV"
BASE COUNT          605 a          626 c          679 g          725 t
ORIGIN      
        1 tgaatacatc cgtgtaggct cgtcaaaaag atccctcttt ttgacggtct gaaatgatgt
       61 gtattcattg cttccgcaca gctagtaacc cggcactaaa tgccgccgag ttaataactc
      121 atcgcattgg ccttcagcgg gggggaattt ttgggcgtgt tggttgtata aaaccgccct
      181 taattacatc gccttagtgg ggcaaacctt acagagcatt gtggtatttg ttgcaaaacg
      241 ctgctgttaa atctaatcca gttccttggc gtgcgctatt tccgctgcag gcgtgaatat
      301 tagccagtgc acgcatacct tctgatagcg gcacacttcc tgtccgtaat aacctcactc
      361 cagtctgtat caggttcata agtttgcagg ccttggcgat tggatcatta attgctgatg
      421 tcctcactgc cggtcagagt aatgagtgtt ttatgccagg cccttgctat ttaggtattt
      481 taggccgctg attgctcaga attttgcatc cgcacgcctc aatggcaatt gaactgcaat
      541 ggccgcaaga gaatttcgcc tgatgatgaa tttttatatg acgccgggtt cctgctcgac
      601 gggcattcat attttattgg aagaactcga attggttttt agcgcccagg tggtaaatct
      661 gctcgctggg gacacacaaa aacccgcatt tttagccatt aacccgaagg caaccatccc
      721 ggtacttgtg cgtgatgacg gcactgcgct gaccgatttt atcgccatcg cctggtggct
      781 tgccgctacc tatccccagg ccaaattatt gccggagggg ctggataaac aggcggaagt
      841 gttaaatgtg atgaacgata ttgtcagtgg tattcacatg cagggtttta cgcggatatt
      901 taccccggag cgttacatgg cgcgcgaggg tgataaaagc tgggtggaag cccaaggccg
      961 cagctgtgtg gataaaggct tggccttgat tgaaaggcgt ttacaggcca agcccggcta
     1021 tttatttggc cgctttggga ttgcagatgc ggctttgttt tatgtggaat tttgggcgtc
     1081 gcgcattaat ttagcgttgc ctgccgcctg cgccgagcat ttgcgcctga tgcaatctcg
     1141 cccggtggta aagcgggttt tggccgaaga gggttatcgc atttaaaagc ggctgagtgc
     1201 gcctaaagtt tgagtcttat ttttgagtta cccaagtttg gctgctgccc aggttgattc
     1261 cccgtgaccg ttaatttatg agttgtgcgg gaaattagac gtttatcgct ggtatttttt
     1321 gcagccaatt ttgcacccag agggctgcgg ccaatgggta attatccacg gattgagcat
     1381 cgagcaagcc tatatcaccg atgcgttttg cgccgcagct gtagaaggca ttttccaaac
     1441 taatgccgcc tagggcgtat tggctgggtg ggaaataact gtctccgagg gcgatgacgc
     1501 cgtaatggcg attagaaaga tccagttgtt gatcttccag ggcgcggtag aggggataaa
     1561 tgttgtaggg caatttgcct tggtcggtgg tggaacaaca ggcgaggata atttcgtctg
     1621 catcccgcag taaatccgcc ggttgagttt ccacattaat ttgggtgctg tggccgagtt
     1681 ttttcagtag ggccgcagcg ccttgggctg cggcaaaggc gtggccattt accgtgccca
     1741 ccacaatgag aaatttcgcc atggtatgtc tccagattgc gcgtttggaa tgggtttcat
     1801 acaagaccgc aaaatgtgta gtgggccttt gtaggattca ttacgttttt cacctcatca
     1861 gcaaataaca cacccatatg aaatattcag aagttatccc ttatatttta agttttgatc
     1921 aggtcacgcg ccgcaccatt agcgagagcg gtacggctat tgtattttgc ctgcaggagc
     1981 gcatttttgc ttattttgaa accggcgcac cgattcaatg gaaactgagt ttagaagtga
     2041 gcgaggcgca attgagagaa ttaatttatc cgccgcaaat tacccacgcc aaagacaaac
     2101 ctgcaggcca ttggattacc attgcgcggg tggaaaattg cgatgaagaa ttgttgaaag
     2161 aattaatcgc cggttcctat gcccgcgcgc aggcaaaaaa tgctacgcct gtaggcgtgt
     2221 aaaaccagcc aaaaatgagg ccgcgctttt ttataaccgg ccttaggcca accgctgctc
     2281 aggcgcttgt ccaaagcttg gtattacccg ctattgataa gtacaccgct attgcttcac
     2341 cccctgagta atatatccag ctcggcatga ttgcttgccg ctccgccatt acccagcgcg
     2401 tcgctgtacc ttgccctcat cgaatccggt cattggcgcg taggcaatag aggccgcagc
     2461 ggcctagttc tgctgcctta aaccctaggg aacctccgaa aaattacctc actcgcccta
     2521 gcgtcgttga atttggcaaa aaaatgctca tttactcggt gtaaactcgg caattgctct
     2581 tgcattgctc tacctcctgc atccatgcag tcgtcggcaa ttgctcctgc attgc
//
LOCUS       DAOQGS010000581         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24473_length_2634_cov_4.359442, whole genome shotgun sequence.
ACCESSION   DAOQGS010000581 DAOQGS010000000
VERSION     DAOQGS010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24473_length_2634_cov_4.359442"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1387
                     /locus_tag="PKZ52_16495"
     CDS             320..1387
                     /locus_tag="PKZ52_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNG61424.1"
                     /translation="MGLSSKLCIPKSLVLGTASLLLASVISGCGGNPDTGSSSSSSSS
                     SSSSSSSSSTGKPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGNLISTKPT
                     SGQKTVTVKGMSRSYNLYVPSSYPTDKRVPLIIRFHGLGGSGSSEITSTGYKAIADQE
                     GLILVAATGASGPSGNAWNVGPCCTSDKNVDDVAFAKAMVEEVKKVANIDTSRVYATG
                     FSMGGGMTHYLACHASDVFAAFAPEAFDLLKENVDQCKPGRAVPILAQRASNDSLVKY
                     AGGQDCLTGTCINFLGAVGTNDVWKQKNGCTAVTKTDGQCVINTQCTNGVETGLCTNT
                     LGHAPASATIGWNFLKKFTLP"
     gene            complement(1657..>2634)
                     /locus_tag="PKZ52_16500"
     CDS             complement(1657..>2634)
                     /locus_tag="PKZ52_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNG61425.1"
                     /translation="GASFGWYMPAHGYDQFWPKMGVDYDGANPAFNDLYHTGTDTPYK
                     NQSTWYTTNPQSQQHWFNRINDLVTRYKPDLLYTDGGIPFGDVGRQLVANLYNTSAAG
                     HKGVNQAVFTHKDMGSGEFIREAGIQDVERGGMGDINPTPWQTDTSIGDWFYSEGFAY
                     KTTDQIVHTLVDVVSKNGNLLLNVVLYPDGSLPPEPQHFLTEMSAWMAVNSEAIYATR
                     PWKIYGEGPTKAEAGHFKESDLFTEADIRFTTKGKTLYAITLRPPTQPIRILSLGKNS
                     RFIGSAVKSVRMLGHKKPLQWQQTNDALVVQIPPQLPSLYGSSLKIEFQ"
BASE COUNT          683 a          642 c          600 g          709 t
ORIGIN      
        1 gtttcgctag gtcgacggca tagatgcagg agtgccccag ggcgcagcag gcagatcctg
       61 tggaggcaaa attgcgcttg ttttttaggt ataaaatgat acggctggca aaataaaagt
      121 gcgcaatcgt tcgcaaatat actcacaagc tgcgtgtccg tcgccccgct cacaggttcc
      181 tcacaattaa gctttatagt ccctgtcgta cattcaggaa gaaattaaaa gtcacatagt
      241 cgtgtgcaat ataagggaga tgttgttcag cctagatgtg tcgaaactca caataaaata
      301 gactaaaaca ggtgccttta tgggtttatc atcaaaattg tgcataccta agagccttgt
      361 gcttggcaca gcttctttat tgctcgcctc agtgatcagc ggttgcggcg gcaacccaga
      421 tacgggttcg tcgtcttcca gctccagcag ctcatccagt tcaagctcca gctcgtcaac
      481 cggcaaacca agctcttcct ccagctctag ctcatcctct agcagttcaa gctcatcctc
      541 cagcagcagc tctagttcat cgtccagtag ctcaagttca tcctccagca gctcatccgg
      601 taatttaatt agcactaagc caacctcagg gcaaaaaacc gttacggtta aaggcatgag
      661 ccgcagctat aacctctacg tgcccagctc ctacccaact gataaacgtg ttccgttgat
      721 tattcgcttc cacggcctcg gcggcagcgg cagctcagaa attacctcca ccggctataa
      781 agccattgcc gatcaagaag gcttaatttt agtagccgct acaggtgcct ctggcccgtc
      841 gggcaatgcg tggaacgtag gcccttgctg tactagcgat aaaaatgtag atgacgttgc
      901 gtttgctaaa gcgatggtcg aagaggtgaa aaaagttgcc aatatcgata cctcccgcgt
      961 gtacgccacg ggtttttcca tgggcggcgg tatgacccat tatttggcct gccacgcatc
     1021 cgatgtattc gcagcctttg cacctgaagc cttcgattta ttaaaagaaa acgtcgatca
     1081 gtgtaaacca ggccgcgctg tacccatttt ggctcaacgg gcttccaacg atagtttagt
     1141 taaatatgct ggcggccaag attgtttaac cggcacctgt attaactttt taggtgcggt
     1201 gggaactaac gatgtgtgga aacaaaagaa cggttgtacc gcagtcacta aaaccgatgg
     1261 ccaatgtgtc atcaatactc agtgcaccaa tggtgttgaa accggcttgt gtactaatac
     1321 cctcggccac gcaccggcca gtgccactat tggttggaat ttcttaaaga aatttactct
     1381 accctaagta aaaaagtagg aagtgcctgg gcgtaacagc aggcccattc ctacatattc
     1441 cgagctggaa atagagggct atttggggcg ccatcctagg atgagcgccc cttttttttt
     1501 ccgttttatc ggtgttttat aaagagtaaa ttctttcacg cctaaatggt gccgcatttt
     1561 ccaacctata aataaacggc tcgtttcgct atttaaatcg tgttggcttt agctgctttg
     1621 cgattgactg gttaaaactg agctgaaaaa taactattat tgaaattcaa ttttcaaact
     1681 agacccatat aggcttggca gttgaggtgg aatctgcacg actaaggcgt cattggtttg
     1741 ctgccattgc aagggctttt tatggcctag catgcgtacc gatttaacgg cactgccaat
     1801 aaagcgacta tttttgccca aggataaaat acggatcggt tgggtgggag ggcgtagcgt
     1861 aatcgcatag agggttttac ctttggtggt aaaacgaatg tcggcctcgg taaataaatc
     1921 actttcttta aaatggcctg cttcagcctt ggttggccct tcgccataaa tcttccaagg
     1981 ccgcgttgca tatatagctt cgctatttac cgccatccag gcgctcattt ccgttaaaaa
     2041 atgttgcggc tcaggcggca aactgccatc cgggtagagc actacattta ataataaatt
     2101 gccatttttg ctcaccacat ccacaagtgt gtgcactatc tgatccgtag ttttataggc
     2161 aaatccttca ctataaaacc aatcaccaat ggaagtatca gtttgccaag gagtaggatt
     2221 aatgtcgccc atgccgccgc gctccacatc ctgtatgccc gcttcgcgaa taaattcgcc
     2281 tgaacccata tctttatggg taaataccgc ctgattcacg cccttatggc cagctgcact
     2341 agtgttgtaa aggtttgcta ccaattgccg ccccacatcg ccaaatggaa tgccgccatc
     2401 ggtatagagt aaatcaggtt tatagcgcgt gactaaatca ttaatgcgat taaaccagtg
     2461 ctgctgagat tgtgggttgg tggtatacca ggtgctttgg tttttataag gtgtatctgt
     2521 acctgtgtgg tacaagtcat taaatgcagg attagcgccg tcataatcca cgcccatttt
     2581 cggccaaaat tgatcataac cgtgggcagg catataccag ccgaaacttg cgcc
//
LOCUS       DAOQGS010000582         2620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24682_length_2620_cov_5.084600, whole genome shotgun sequence.
ACCESSION   DAOQGS010000582 DAOQGS010000000
VERSION     DAOQGS010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24682_length_2620_cov_5.084600"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1289
                     /locus_tag="PKZ52_16505"
     CDS             177..1289
                     /locus_tag="PKZ52_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG61426.1"
                     /translation="MTNKILYPTLPLFLALALSPPICAGERQDIFPVTAQQMQAMSIQ
                     VKALQQNAEPVVLSAPAQVTVPINRQQVVSASLPGLVLHVLVQPNQLVKQGDPLVKIA
                     SQEFGALQLQWVEANNRVKLARKAAEREKSLFDEGVIAQRRVQETQAALADSEAALTQ
                     AKAALNMIGLSQAAITRIGEGKLEEALVLVAPQSGAVTELAIKPGQRLEPMTALMNIA
                     QTDKLALDIQVPSGTAAAWTAGAPLTLQGHAGTGKIVSASPVVSAGSQSVYVHGELDA
                     KDVDVRSGQLVTVQLPLPADKDSWDIPLAAVVYDAAEAHVFVRTNEGFEARPVKVIMS
                     AGQRVRVQGSIKAGDAIAVTGVIALKGSWLGEKGGE"
     gene            1289..>2620
                     /locus_tag="PKZ52_16510"
     CDS             1289..>2620
                     /locus_tag="PKZ52_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61427.1"
                     /translation="MLTQLVQFSLSQRLFVLLATLIIGTGGWYAFSQLPIDAFPDVAS
                     TQVKVIMKAPGMTPEEIENRIAVPIEVEMLGIPNTKILRSVTKYGLADVTIDFQDGTD
                     LYWARQQVSERLSGITDSLPADISGGMAPITTPLGEMFMFTVEGKGLSLAERRSLLDW
                     VIRPALRTVPGVADVNSLGGVVRAFEVIPNENAMAAAGVSLTQLKDAIRANNRNDGAG
                     RLGEGDEVLLVRSEGSITSLDDLRAVVVTVKNEVPIRVDALADVQIGTSTRYGVVTQS
                     GQGEAVQGLVLGLAGANAQKVVEGVRKKLTEIQPTLPQDVTINVFYDRAELVSKAVGT
                     VSSALLEATVLVIILLGLFLGDVRAAVTVALVLPLAALLTFILMRFFNMSANLMSLGG
                     LSIAIGMLVDAAVVMVENIVQRLGHDPTAGRVPRLHTIYRAVCEVAVPVTSG"
BASE COUNT          591 a          643 c          752 g          634 t
ORIGIN      
        1 gtacagcggg tacgagattt tcgcatcact tttgttgcgt gtggccatcg cccttatcta
       61 aaagtttttg acggctcagc tgcgcatcgt gtgcatctga tcgctttgat acttttacat
      121 ttaaacggtt aatgcacgcg gctttgcgct gtgggcgatt tctcaaagag agcaccatga
      181 cgaataaaat tctttacccc acccttccct tatttctagc gctggcgctg tctcccccga
      241 tatgtgcggg cgagcgtcag gatatatttc ctgtaacggc tcagcagatg caagcgatga
      301 gcatccaagt aaaagccctg caacaaaacg cggaacctgt agtgttaagt gcacctgccc
      361 aggtaaccgt gcctattaac cgccagcagg tggtgagtgc atccttgccg ggcctggttt
      421 tacacgtatt agtacaacct aaccagctgg tgaaacaagg tgatccgtta gtaaagatcg
      481 ccagccagga attcggcgcg ttgcaattgc aatgggtgga agcgaataac cgcgtcaaac
      541 tcgctcgtaa agctgcagag cgcgagaagt cactgtttga tgagggtgtt atcgctcaga
      601 ggcgtgtgca agaaacccaa gccgccctgg cggacagtga ggctgcgtta actcaggcaa
      661 aagccgcact caatatgatt gggctttctc aagcggccat tacccgcatt ggtgaaggca
      721 agctcgaaga agctttggta ctggttgccc cccaaagcgg tgccgtaacc gagcttgcga
      781 ttaaacctgg ccagcgcctg gagcctatga cagcgctgat gaatatcgcc cagaccgaca
      841 aattggcatt ggatatacaa gtgccatctg ggacggcggc tgcttggacc gccggtgcgc
      901 cgctcacgct gcagggccac gcgggtaccg gaaaaattgt cagtgcaagc ccggtggtaa
      961 gcgctggaag ccaatcagtt tatgtgcatg gtgaattaga tgcgaaggat gttgatgtgc
     1021 gctcaggcca actggtcaca gtgcaattgc cactgccagc cgataaagac agttgggata
     1081 tacccctagc cgctgtagtt tatgacgcag ctgaagctca cgtatttgtt cgcactaacg
     1141 agggctttga ggcgcgcccg gtgaaagtga ttatgagtgc tggccaacgg gttcgagtac
     1201 agggctcaat aaaagcggga gacgctatcg ctgtaacagg tgttattgcc cttaaaggtt
     1261 cctggctcgg cgaaaaggga ggcgaataat gctcacgcaa ttagttcagt tctccctgtc
     1321 tcagcggctt tttgtgctgc ttgcgacact gattatcggt actggaggct ggtatgcctt
     1381 tagtcaactg ccgattgatg ccttccccga tgtggccagc acgcaagtga aggtcattat
     1441 gaaagccccc ggtatgacgc cggaagaaat cgaaaaccgc atagccgtgc ccatcgaggt
     1501 tgagatgttg ggtattccca atactaaaat cctgcgttcg gtgaccaaat acggccttgc
     1561 tgatgtcacc atcgattttc aggatggcac tgacctttat tgggcgcgcc agcaagtcag
     1621 cgaacgcctc agcggtatca ccgatagttt gcccgctgat atcagcggtg gcatggcccc
     1681 cattacaact ccgctgggtg aaatgtttat gttcactgtg gagggtaaag gcttatccct
     1741 ggccgagcgg cgcagcctat tggattgggt gattcgtcca gccttgcgta cggttccagg
     1801 cgtggccgat gttaactcct taggcggtgt ggtacgcgcc tttgaagtca tccccaatga
     1861 aaatgccatg gccgcagcgg gtgtgagcct tacccaattg aaagatgcca tccgcgctaa
     1921 taatcgcaac gatggcgctg ggcgcttggg tgaaggtgat gaggttttat tagtccgcag
     1981 cgaaggcagc atcacgagcc tggatgatct gcgtgcagta gtcgtaacgg taaaaaatga
     2041 agtacccatc cgagttgacg ccctggcgga tgtgcaaatt ggtaccagca cgcgttacgg
     2101 tgtcgttacc caaagtggcc aaggcgaagc cgtgcaagga ttagtattgg ggttagcggg
     2161 cgcaaatgcg caaaaagtcg tggagggtgt gcgtaaaaag ctgacggaaa tccagccaac
     2221 cttaccgcag gatgtcacga tcaatgtctt ttatgatcgt gctgaattag tctccaaagc
     2281 agtgggcaca gtgtcctcgg cattattgga agccacagtg ctggtcatca ttttgctcgg
     2341 gttgttttta ggggatgtgc gagcggcggt gacagtagcc ttagtactgc ctctggcggc
     2401 gctgctgacc tttattttga tgcgcttttt taatatgtcc gccaacttga tgagcctggg
     2461 cggattgtct attgcgatcg gtatgttggt cgatgccgcc gtggtgatgg tggaaaacat
     2521 cgtgcagcgc cttggccatg atcctaccgc tggccgggtt cctcgattgc acaccattta
     2581 tcgagcggtt tgcgaagtgg cggtgcctgt tacctcgggt
//
LOCUS       DAOQGS010000583         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24699_length_2619_cov_5.190718, whole genome shotgun sequence.
ACCESSION   DAOQGS010000583 DAOQGS010000000
VERSION     DAOQGS010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24699_length_2619_cov_5.190718"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKZ52_16515"
     CDS             <1..599
                     /locus_tag="PKZ52_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61428.1"
                     /translation="PMDSFLQQHFILGLILFLTFSYALGLVWQAVMQRIWGGSILKEM
                     GNERALYLKTRQGEFRVDGTHKRFSFKPKKAASWEAVDFDQVRNIHIFREVNGGALIE
                     FLLGDWGLWDLHERYRDVINTYTIQITLKDSRDIAIFVLRQYEQREMWLGHFFLNISL
                     GILKALHLYKDIEEEAEEKLDHIVRAFKAVGFDINVRH"
     gene            777..1379
                     /locus_tag="PKZ52_16520"
     CDS             777..1379
                     /locus_tag="PKZ52_16520"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin C"
                     /protein_id="HNG61429.1"
                     /translation="MGVLVGKPAPDFTAAAVLGNGEIVDSYSFSAATKGKKAVVFFYP
                     LDFTFVCPSELLAFDSRVGEFAKRGVEVIGVSIDSHFTHNAWRNTPVNEGGIGPVKYT
                     LVADINHAICKAYDVESEGGVAFRGTFIIDEEGVVRSQIVNDLPIGRNVDETLRTIDA
                     LIFHQEHGEVCPAGWKDGDKGMVATPEGVAAYLAENASKL"
     gene            1508..2452
                     /locus_tag="PKZ52_16525"
     CDS             1508..2452
                     /locus_tag="PKZ52_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNG61430.1"
                     /translation="MPSKIPLGISACLLGDAVRFNGGHKKSDFCTQLLQNYFEFQRFC
                     PEVAIGLGVPRQTIRLVGDFAAPRAVVPATAADLTEPLADYGREVAAQARHFSGYILM
                     KDSPSCGLFSTKVYSGQSPLPGKRAGIFAQALREQLPLLPMEEEARLNDPGLCENFIA
                     RVYAYHDWRLNFLPNASPQQLVNFHSRYKYFVMAHGQALYTQLGRLVAGAGVGDFDQL
                     VHSYEQAFFIGIIKPPTRRNHVNVLYHILGYLKKQVAANLKQDLLKAVEDYRQTLVPL
                     IVPITLLKHYLTHYACDYINQQVYLTPYPEQLKLRNQL"
BASE COUNT          629 a          700 c          650 g          640 t
ORIGIN      
        1 tacccatgga ttcattttta cagcaacact tcattttggg tttaattcta ttcctaacct
       61 tttcctacgc gctgggcctt gtctggcagg ctgttatgca acgcatatgg ggcggcagca
      121 tactgaaaga aatgggcaat gagcgcgctt tatatttaaa aacccgccaa ggtgagttta
      181 gagtcgatgg cacccacaaa cgctttagtt ttaagccgaa aaaagcggcc agctgggaag
      241 cggtggattt tgaccaggtg cgcaatattc atattttccg tgaagtgaac ggcggtgctt
      301 taattgaatt tttgctgggt gattgggggc tgtgggattt acatgaacgc taccgcgatg
      361 ttatcaacac ctacacaatt caaattaccc tcaaagacag ccgcgatatt gccatttttg
      421 ttctgcgcca atacgaacag agggaaatgt ggctggggca cttttttctc aacatcagcc
      481 tgggcatttt gaaggcgcta catctttata aagacattga ggaggaggcc gaggaaaaac
      541 ttgaccatat tgtccgggca tttaaggcgg tgggctttga tattaatgtg cggcactgat
      601 acccccaacc cgctgaggtg tattgcctgt ctctatagaa cccatataaa aattttatct
      661 aatgttgcgc cgcaacgagg tatgattgcc tcaggctatc aggtttacat gcctgattga
      721 tgtttacccc aaaagccttt agtcaaccct ctaaccttaa caataggaga acccttatgg
      781 gcgtattagt aggcaaaccc gcacccgact tcactgccgc tgccgttttg ggcaatggtg
      841 aaattgttga tagctacagt ttttccgctg ccaccaaagg caaaaaagcc gtagtgttct
      901 tttacccact ggacttcacc tttgtctgcc cgtctgagct gttggcgttt gatagccgtg
      961 taggtgaatt cgctaagcgc ggcgtggaag tgattggtgt atccatcgac tctcacttca
     1021 cccacaacgc ctggcgcaac acccctgtga acgaaggcgg tattggcccg gttaaataca
     1081 cactggtggc cgacatcaac cacgctatct gtaaagccta tgacgtagag tcagaaggcg
     1141 gcgttgcctt ccgtggcacc tttatcatcg acgaagaagg cgttgtgcgc tcacagatcg
     1201 ttaacgacct gcccatcggc cgcaatgtgg acgaaaccct gcgcaccatc gacgcgctga
     1261 ttttccacca agagcacggc gaagtatgcc cagctggctg gaaagacggt gacaaaggca
     1321 tggttgccac ccctgaaggt gtagccgctt acctggcaga gaacgccagc aagctctaat
     1381 cctttaatgc ctgtaacacg taaaaccccg gatactcatc tggggttttt tttgccctaa
     1441 attctggtta gcccaaaagc gcccataatt aactcacccc cttaaccgct cgcgcgaggt
     1501 tgtgcccatg ccgtcaaaaa ttcccctagg catttccgcc tgtttgttgg gtgatgcggt
     1561 gcgctttaac ggcggccaca agaagtcgga cttttgcaca cagctgctgc agaattattt
     1621 tgaattccaa cgcttttgcc cggaggttgc cataggcctt ggggtgccga ggcaaaccat
     1681 tcgcctggtg ggggattttg cagcccccag agccgtagtg cccgccaccg ctgcggacct
     1741 gaccgaaccc ctcgccgact acggccgaga agtcgccgct caagcgcgac actttagcgg
     1801 ctatatattg atgaaagatt cccccagctg tggcttgttt agcaccaagg tctactcagg
     1861 ccaaagcccc ctgcccggta aacgggccgg catatttgcc caggccctgc gggagcaact
     1921 gccgcttctt cccatggagg aagaggcgcg tttaaatgac ccgggcctgt gcgaaaactt
     1981 catcgcccgc gtttatgcct accacgattg gcgcctgaac ttcctgccca atgccagccc
     2041 ccagcagctg gtgaactttc acagtcgcta taagtatttt gtgatggccc acggccaagc
     2101 cctctacaca caactggggc gattggtggc gggtgcgggc gtcggggatt ttgatcagct
     2161 agtgcactca tatgagcagg cgttttttat cggcatcatc aagccgccta cccggcgcaa
     2221 tcacgtcaat gtgctctacc acatactggg gtacttaaaa aaacaggtgg cggccaattt
     2281 aaaacaggat ttactcaaag ctgtggagga ttaccggcag actctggtgc ccttaattgt
     2341 gcctattacc ctgttaaaac attacttaac ccactatgcc tgcgactaca ttaaccagca
     2401 ggtttattta acgccctacc cggagcaact gaagttgcgc aatcagctgt aagcctttaa
     2461 aaataaataa aatactggtt gacgcaactg cgccagatcc ctagaatgcg cgacctcagc
     2521 tgatgcaaca caaacagctg aacccggtgc ggggtggagc agcttggtag ctcgtcgggc
     2581 tcataacccg aaggtcgttg gttcaaatcc agcccccgc
//
LOCUS       DAOQGS010000584         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24787_length_2613_cov_4.198202, whole genome shotgun sequence.
ACCESSION   DAOQGS010000584 DAOQGS010000000
VERSION     DAOQGS010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24787_length_2613_cov_4.198202"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2613)
                     /gene="gltB"
                     /locus_tag="PKZ52_16530"
     CDS             complement(<1..>2613)
                     /gene="gltB"
                     /locus_tag="PKZ52_16530"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNG61431.1"
                     /translation="CLGRELSVFAETAEHAERVILSSPVLTPAKFRALLAVDAKRINP
                     KTFKLHYNPEEKSLQQAVNDLVEEAVAAVKQGYITLVLSDFGMQQGLLPIHALLATGA
                     VHQRLTQDSLRCDANLVIETATARDPHHVATLIGFGATAVYPYLSYYVINHLVETGDL
                     LVDISTAYKNYHKSIDKGLLKIISKMGISTVASYRGAQLFEAIGLAEEVVDTCFAGTP
                     SRIKGAGFADLEADQKALAADAFKARKPIAAGGLLKYVHGQEYHAYNPDVVQTLHRAV
                     QSGDYAAWRDYAAVVNNRPVATLRDMFKLKDGIKPIPLSEVEPVENLFKSFDSAGMSL
                     GALSPEAHEALAEAMNRLGGRSNSGEGGEDPARYGTVKSSKIKQVASGRFGVTPAYLV
                     NAEVLQIKVAQGAKPGEGGQLPGGKVNELIARLRYSVPGVTLISPPPHHDIYSIEDLA
                     QLIFDLKQVNPQALISVKLVSRPGVGTIAAGVAKAYADLITISGYDGGTAASPLTSIK
                     YAGSPWELGLSETHQTLCANDLRGKVRVQTDGGLKTGLDVVKAAILGAESFGFGTAPM
                     VALGCKYLRICHLNNCATGVATQQDKLRQDHYIGTVEMVMNFFRFAAEETREWMARLG
                     VRSLKELIGRVDLLEVIEGQTPRQSKLDMSPILYSDDLIKSKPQYCQVARNEPFDQGL
                     LAERIVADVKGAIAGKTGGSWDYTITNCDRSIGARVSGEIAKVHGNLGMESAPIRLNF
                     IGVAGQSFGVWNAGGLEMYLEGDANDYVGKGMAGGKLVIRPPRNSSFVAHETSIVGNT
                     CLYGATGGKLFAAGGAGERFAVRNSGAHAVVEGAGDHCCEYMTGGVVTVLGPTGVNFG
                     AGMTGGFAYVLDEE"
BASE COUNT          524 a          754 c          769 g          566 t
ORIGIN      
        1 tttcttcgtc caacacatag gcaaagccgc cggtcatacc cgcgccgaag ttaacacccg
       61 tagggccaag cacggtaacc acgccgccgg tcatgtattc acaacagtga tcccctgctc
      121 cttccaccac cgcatgggcg ccggagttgc gcacggcaaa gcgctcaccg gcgccgcccg
      181 ctgcgaacag tttgccgccg gtggcaccgt acaaacaggt gttgccgacg atggaggttt
      241 catgggccac aaaacttgag ttgcgtggtg ggcgaatcac cagcttgccg cccgccatgc
      301 ctttacctac atagtcgttg gcatcccctt ctaaatacat ttccaggccg ccggcgttcc
      361 aaacgccaaa gctttggccg gccacgccga tgaagttcaa gcggatgggg gcggattcca
      421 tgcctaggtt gccgtgtact ttagcaattt caccactgac acgggcgccg atagagcggt
      481 cgcagttggt gatggtgtaa tcccagctgc cgccggtttt gcctgcgata gctcctttaa
      541 catccgccac aatgcgctcg gctaataggc cttggtcgaa gggttcgttg cgcgccacct
      601 ggcagtattg gggtttgctc ttaatcaggt cgtcgctgta gagaatcggc gacatatcca
      661 gtttgctttg acggggggtc tgcccctcga ttacttctaa cagatctacc cggccgatca
      721 gctcttttaa gctgcgcacg cccaggcggg ccatccattc acgggtctct tcagcggcga
      781 agcggaagaa gttcatcacc atttccactg tgcctatgta gtgatcctgg cgcagcttgt
      841 cctgctgggt agccacgccc gtagcgcagt tgttcaggtg acagatgcgc aggtacttac
      901 agcccaaagc taccatgggg gcggtaccaa aaccaaaact ttcggcgccc aaaatagcgg
      961 ccttgactac gtccaaaccg gttttcaggc cgccgtcagt ctgcacccgc accttgccgc
     1021 gcaggtcgtt ggcgcagagg gtttgatggg tttcagacag gcccaactcc cagggtgaac
     1081 cggcgtattt aatagaggtg agcgggcttg ccgctgtacc gccgtcgtaa ccggagatag
     1141 taatcaaatc cgcataggcc ttagccaccc cggcggcaat ggtgccgacc cctgggcggg
     1201 agacgagttt gacggagatg agggcctgtg gattcacctg ttttaagtcg aagatcagct
     1261 gggccaagtc ttcgatggag taaatatcgt ggtggggcgg aggcgaaatt agggttacgc
     1321 cgggcaccga gtagcgcagg cgcgcaatca gttcatttac cttgccgccg ggcaactggc
     1381 cgccctcacc gggcttggcg ccctgggcga ctttaatttg cagcacttcg gcgttaacca
     1441 agtaggcagg tgttacgcca aaacggccgg aggccacctg tttgatcttg gagcttttca
     1501 ccgtgccgta acgggcgggg tcctcaccgc cttcaccgga gttggagcgg ccacctaaac
     1561 ggttcatggc ttcggccagg gcctcgtggg cctcgggtga aagcgcacct aaggacatgc
     1621 ccgccgaatc gaagctttta aagagatttt ccaccggctc aacttcggac agcggaatcg
     1681 gcttgatgcc gtcttttaat ttgaacatat cccgcagggt ggccactggg cggttgttca
     1741 ccaccgcagc gtaatcgcgc caggcggcgt aatcaccgga ctgcacagcc cggtgcagag
     1801 tctgcaccac atccgggtta taggcgtggt actcctggcc gtgtacatac ttaagcaggc
     1861 cgcccgcagc tattggtttg cgcgctttaa aggcatcggc ggcgagggct ttttggtcgg
     1921 cttccaaatc ggcaaagcct gctcctttaa tgcggctggg ggtaccggca aaacaggtgt
     1981 ccaccacttc ttcggcaagg ccaatggcct caaataactg ggcgccccgg taggaggcca
     2041 cagtggagat acccattttg gagatgatct ttaacaggcc tttatcgata ctcttgtggt
     2101 agttcttata ggcagtgctg atgtccacga gcagatcgcc cgtctctacc aggtggttga
     2161 tcacgtagta gcttaaatag gggtacacgg ctgtagcgcc gaagccgatc agggtcgcca
     2221 catggtgtgg gtcccgggcg gtggcggttt caatcactag gttggcgtcg cagcgcaggc
     2281 tgtcttgggt taaacgctgg tgcacagcgc cggtggccag cagcgcgtgg atgggcaata
     2341 gcccctgctg cataccaaaa tcgctcaata ccagggtgat ataaccctgc ttcacggcgg
     2401 caaccgcctc ttccaccaag tcgttaacgg cctgctggag ggatttctcc tccgggttgt
     2461 aatgcagctt gaaggttttg gggttgatgc gtttagcatc caccgccaac agggcgcgga
     2521 acttggcagg cgttaacacc gggctggata aaatcacccg ctcggcgtgc tcagcggttt
     2581 cggcaaacac cgacagctcg cggcctaggc atg
//
LOCUS       DAOQGS010000585         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24796_length_2612_cov_5.072350, whole genome shotgun sequence.
ACCESSION   DAOQGS010000585 DAOQGS010000000
VERSION     DAOQGS010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24796_length_2612_cov_5.072350"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKZ52_16535"
     CDS             <1..724
                     /locus_tag="PKZ52_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61432.1"
                     /translation="IGGGRLDTILYDPEYKKRYEVEIQLGKTDESHIIRTIEYWDIER
                     KKNPQYEHCAVIIAEDITSRFLNVISLFNGFIPLIAIQVKAVKVGDNISLFFTKVLDE
                     NKFDLLDEDAGAESTDKEYWERKATKESLRLTEKVMSLLGEVTSGYNLKFNKHYIGLA
                     KNNIANNFISFVPRKSAVLLYVRLDKTDEIDELIEGSDVELLTYDKQWNQYRLRIKET
                     DLSANADLIKDLAARAKEEYMS"
     gene            complement(925..1602)
                     /locus_tag="PKZ52_16540"
     CDS             complement(925..1602)
                     /locus_tag="PKZ52_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61433.1"
                     /translation="MSELANIISDVVASPLGEVIAAVGQGVADAQQALDEGSLAKTLE
                     IYSEGGSQMLQMLRDIGYNPTFYTLPETTGEVRIALRLGSNQTSVPNGTPQVNASLAR
                     QGLNLPALGNKLYATPVDAGYANRFGYQADISAKLTFKIVPVPAPNGVDELRVVPNLV
                     ESKVGEANNRLAALQLAVRWVDEKGEALQTIDEGQIVISQTPSGQGDRPAILRSGQEV
                     VLQLKAI"
     gene            complement(1626..>2612)
                     /locus_tag="PKZ52_16545"
     CDS             complement(1626..>2612)
                     /locus_tag="PKZ52_16545"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNG61434.1"
                     /translation="LCDFGQVEAFGDQAYSLQAVQSQFANQPLQIAGAPKHAVLNTAV
                     LMRAAVLNPAAASALARGSLDASALTLEASAAAVTAARDAAIAVAQPAEIDSSALGTF
                     NAEMIKFKALETQLQLQISDKDRQLQSKDLALQAKAVELQTVQSESLALKSELAQIKA
                     SEAQLKVQNEEFKAEAVRLNPIQTIASNIGAQVAAANQKLQADQKPYQIQNIQVELRG
                     TLSGDGQAMNLVKLGELKEGLAHTSLVRLDLTSSNTKPVENTTLVPDVTGFTESVVRR
                     LLSSVGLRMEKITRSAAGLKNATPGQALSQSPAPGSAVPRNEAVLVVFAVEP"
BASE COUNT          559 a          605 c          757 g          691 t
ORIGIN      
        1 gatcggaggg gggaggctcg ataccattct ttatgatcca gaatataaga agcgatatga
       61 ggttgaaatt cagcttggta aaacggatga gtctcacatt attcggacga ttgaatattg
      121 ggatattgag cgaaaaaaga accctcaata tgagcactgt gcggttatta ttgctgagga
      181 tattacaagt cgttttctaa atgtgatttc gctctttaat ggttttatac cgctgatcgc
      241 gattcaggtg aaggctgtta aagttgggga taatatctcg ttgtttttca ctaaggtgct
      301 ggacgagaat aaatttgatc tgcttgatga agatgctggg gcagagtcca ccgataaaga
      361 atattgggag agaaaggcga caaaagaaag cttaaggtta acagaaaagg ttatgtctct
      421 cctcggggag gttacatctg gatataattt gaagtttaat aagcattata ttggtttagc
      481 aaagaataat atcgctaata attttatttc atttgtccct agaaagtctg cggtgctgtt
      541 gtacgtcagg ctggataaaa cggacgagat agatgagttg attgaggggt ctgatgtgga
      601 acttcttacc tacgataaac aatggaatca atataggtta aggattaaag aaactgattt
      661 aagtgccaat gccgatttga taaaagatct tgctgctcgg gctaaagagg agtacatgag
      721 ttagtggtaa ttgctaaatc tgttttgaat atggacggtt tttgtggagt ggcaactgtg
      781 ttcagtcttg catcgttaaa gcgacgccag gctttgattt tccgatgcta atgttaatgc
      841 agtctataaa acggcgttga ttattttgac tttttcttga gaaaaataaa ctgaaaatgg
      901 gctgatctta tccgcctata aatattaaat agccttcaac tgcaacacca cctcctgccc
      961 agagcgcaaa atcgccggtc ggtctccctg gccgctgggg gtttggctga tgacaatttg
     1021 gccctcgtcg atggtttgca gagcctcgcc tttttcatcc acccagcgca ccgccaattg
     1081 cagggcggcg aggcggttat tggcctcgcc cactttgctc tccactaaat taggcaccac
     1141 tcgcagttca tccacgccgt tgggggcagg cacggggacg attttgaagg tgagtttggc
     1201 gctgatgtcc gcttggtagc caaagcgatt ggcgtagcct gcatccaccg gggtggcgta
     1261 gagcttgttg ccaagggctg gcaggttcag gccctggcgg gccaggctgg cattgacttg
     1321 gggggtgccg ttggggacag aggtttggtt ggagcctaag cgcagggcga tgcgcacctc
     1381 gccggtggtt tccggcaggg tatagaaggt ggggttgtag cctatgtctc gcagcatctg
     1441 cagcatttgg cttccgcctt cggaataaat ctccagggtt ttggccagag agccttcgtc
     1501 cagagcctgt tgggcatcag caacgccctg gcccaccgcg gcaatcacct cacctagggg
     1561 ggaggccact acgtcgctaa taatattcgc cagttcactc atactcaggt ctcgtgcttt
     1621 tgggttcagg gctccaccgc aaacaccacc agcaccgcct cattgcgagg cacggcgctg
     1681 ccgggggcgg gggattggga gagggcttgg ccgggggtgg cgtttttaag gccagcggct
     1741 gaacgggtga ttttttccat gcgcaggccc acggagctta ataggcgccg caccacggat
     1801 tcggtaaagc cggtcacatc cggcaccagg gtggtgttct ccaccggttt ggtattgctg
     1861 ctggtgaggt ccagccgcac cagtgaggtg tgggccaggc cctcttttaa ttcgccgagt
     1921 ttcaccaggt tcatggcttg gccatcgccg cttagggtgc cgcgcagttc cacttggatg
     1981 ttttgaatct ggtagggctt ttggtccgct tgcagttttt ggttggcggc ggccacctgg
     2041 gcgcctatgt tgctggcgat ggtttgaatg gggtttaggc gcaccgcttc ggctttgaac
     2101 tcttcattct gtacttttag ttgtgcctca gaggccttga tttgcgccag ctcgctcttt
     2161 aacgccaggc tttccgattg gacggtttgt agctccacag ctttggcctg caaagctaag
     2221 tctttgcttt gcagttggcg gtctttatcg ctgatctgca gctgtaactg agtttccagc
     2281 gctttgaact tgatcatctc ggcattaaaa gtacctaggg cggagctgtc gatctcggca
     2341 ggttgtgcca cggcgatggc agcgtctcgt gcggcggtaa cagctgccgc gctggcctcc
     2401 aaagtcaggg cgctggcatc caggctaccc ctggccaggg cgcttgcagc ggctgggttg
     2461 agcaccgctg cgcgcatcaa caccgccgtg ttcagcaccg catgtttggg cgcgccggca
     2521 atctgcaggg gctggttggc aaactggctt tgcacagcct gcaggctata ggcctgatcc
     2581 ccaaatgcct ccacctggcc gaagtcacac ag
//
LOCUS       DAOQGS010000586         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24867_length_2608_cov_3.857423, whole genome shotgun sequence.
ACCESSION   DAOQGS010000586 DAOQGS010000000
VERSION     DAOQGS010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24867_length_2608_cov_3.857423"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2325
                     /locus_tag="PKZ52_16550"
     CDS             <1..2325
                     /locus_tag="PKZ52_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNG61435.1"
                     /translation="FSLFGLMAFLFPIMLGYQVGLLLRNREQQLIDPVLLGLRFVGLC
                     LVMVASTGLTVQHQGTHSSDMPFSDGGILGASIAQVADHSFGEIGGVVVLFALCLFGL
                     TIFIDISWFAVLEKIGGLTLAGWDRLMALLRRKAVARLAPEALSPQTVELKPLMVAKA
                     REKAVQLAHKINDALPHKQDKPEAPAALPDGKSQPAANALASNAPDAKPQPVAAATAV
                     KFTPKPAYADETSPADDAGERKIKVVTKLPKPVTLSPRVERDKQQALFDEPVEGELPS
                     ITLLDPPSPGAKKGYSEESITYMSRQLEAKLKDFGIAAQVVEVLPGPVVTRFEIQPAP
                     GVKVSRITNLAKDLARSLAVISVRVVEVIQGKSVVGIEIPNEERQMVRLSEVIASSLY
                     DKSKSPLTMALGHDISGQPMIADLAKMPHLLVAGTTGSGKSVGVNVMLLSILYKASPE
                     EVRLMLVDPKMLELSVYEGIPHLLAPVITDMKDAATGLRWCVGEMERRYRLMAALGVR
                     NLAGYNKKILDAQRAGEPILDPLWRPDESGIGQSEPDELGQLPFIVVVIDEFADMMMI
                     VGKKVEQLIARIAQKARAAGIHMILATQRPSVDVITGLIKANVPTRIAFQVSSKIDSR
                     TILDQGGADQLLGHGDMLYLAPGTSLPVRIHGAFVDDHEVHKVVADWKKRGQPNYLED
                     IFSESTESIQVPGYSEEGEGGGDGESDALYDEAVAFVLESRKASISAVQRKLRIGYNR
                     AARLIDQMEAAGVVTAQGHNGNREVIPPGGPSRY"
     gene            2373..>2608
                     /locus_tag="PKZ52_16555"
     CDS             2373..>2608
                     /locus_tag="PKZ52_16555"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNG61436.1"
                     /translation="MRILLALYLAACACSSWAADASAKLISQLQAIDSLKGDYAQTIT
                     DKNGTLVQKTQGDFVIKRPGYFYWASKGTQEQKV"
BASE COUNT          559 a          751 c          747 g          551 t
ORIGIN      
        1 ttctcccttt ttggtctcat ggcctttttg ttcccgatta tgctgggcta tcaagtgggc
       61 ctgctgctgc gcaatcggga gcagcaattg attgaccctg tgctgctggg cctgcggttt
      121 gtcggcctgt gtttggtgat ggtggcctcc acggggctta cggtgcaaca ccagggcacc
      181 cacagcagcg atatgccgtt ttccgacggc ggcatactcg gcgccagcat cgcccaggtg
      241 gcggatcact cctttgggga gatcggcggt gtggtggtgt tattcgccct ctgcctgttt
      301 ggcttaacga tttttatcga tatatcttgg tttgcggtgc tggagaaaat cggcggcctg
      361 accctcgctg gttgggatcg tttgatggcg ctgctgcgcc gcaaagcggt ggcgcgttta
      421 gcgcctgagg ccctttcgcc gcagaccgtc gaattaaaac ccctcatggt ggcgaaagct
      481 cgggagaaag ctgtccagct ggcccacaaa attaatgacg ccttacccca caaacaggat
      541 aaacctgagg cgcctgccgc cctgccggac ggcaaatcgc aaccggctgc caatgcgctg
      601 gcttctaatg cgcccgacgc caagccccag ccagtcgctg cagccaccgc cgttaaattc
      661 acccccaaac cggcctatgc cgacgagact agtccggcgg atgacgctgg cgagcgcaaa
      721 attaaagtcg tcaccaagct gcccaaaccg gtaaccctca gcccccgagt ggagcgggat
      781 aaacaacagg cgctgtttga tgagccggtg gaaggagagt tgccctccat taccctgtta
      841 gacccgccat cccccggtgc taagaaaggt tattcggaag agtccatcac ctatatgtcc
      901 cgccagttgg aggccaagct caaggacttc ggcatagccg cccaagtggt ggaggtgctg
      961 cctgggccag tggtcacccg ctttgaaatc caacccgccc ccggtgttaa ggtcagccgt
     1021 attactaacc tcgccaagga ccttgcccgc tccctcgctg taattagcgt gcgggtggtg
     1081 gaggtgattc agggtaaatc cgtggtgggc attgaaattc ccaacgaaga gcggcagatg
     1141 gtgcgcctgt cggaagtgat tgcctcttcg ctatacgata agtccaaatc ccccctcact
     1201 atggccctcg gccacgatat ttccggccag cccatgattg cggacctagc caaaatgccc
     1261 cacctgctgg tggcgggcac cacgggttcg ggtaaatccg tgggggtgaa tgtgatgctg
     1321 ctgagcatcc tctacaaagc ctccccagaa gaagtgcgcc tgatgctggt ggacccaaaa
     1381 atgttggagc tgtccgtgta tgaaggcatc cctcatttgt tggcaccggt gattaccgac
     1441 atgaaagacg ccgccactgg cctgcgctgg tgtgtgggtg agatggagcg ccgctaccgt
     1501 ttgatggccg ccttgggggt gcgcaacctg gccggttaca acaaaaaaat cctcgatgcc
     1561 cagcgcgcag gcgagccaat tttggacccg ctctggcggc ccgatgaaag cggtatcggc
     1621 cagagcgagc cggatgaact gggacaattg ccgtttattg tggtggtgat cgacgaattt
     1681 gccgacatga tgatgatcgt gggtaaaaag gtggagcagt tgatcgcccg tatcgctcaa
     1741 aaggcgcggg ccgccggtat ccatatgatc ctcgccaccc agcggccctc ggtggatgtg
     1801 attaccggcc tgattaaagc caacgtgcct acgcgcattg ccttccaggt atcgagcaaa
     1861 attgactccc gtaccatcct cgaccagggc ggcgccgacc agttactggg ccacggcgat
     1921 atgctctatt tagcgccggg caccagcctg ccggtgcgca tccacggcgc ctttgtggac
     1981 gaccacgaag tgcacaaggt ggtggcggat tggaaaaaac gcggccagcc gaattatttg
     2041 gaagatatct tcagcgaatc caccgaatca attcaggtac ccggttacag cgaagaaggt
     2101 gagggcggtg gcgatgggga gtcggacgcc ctctacgacg aggccgtggc ctttgtgttg
     2161 gagtcccgca aggccagtat ttcagcggta cagcgcaaat tgcgaatcgg ctacaatcgc
     2221 gccgcccgtt taatcgacca aatggaagct gccggtgtgg taactgctca gggccacaac
     2281 ggcaaccgcg aagtgattcc ccccggcggc cctagccgct actaatttat aaatccacgg
     2341 cttaagccgt tttatctcag attggaaacc ctatgcgaat tctgttagcc ctctatcttg
     2401 ccgcctgcgc ctgctccagc tgggccgccg atgccagcgc caaattaatc agccaattac
     2461 aggccatcga cagcctaaaa ggcgattacg cgcaaactat taccgataaa aacggcactc
     2521 tggtgcaaaa aacccaaggc gattttgtca ttaaacgccc cggttatttt tattgggcca
     2581 gcaaaggcac ccaggagcaa aaagttat
//
LOCUS       DAOQGS010000587         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24900_length_2606_cov_4.722070, whole genome shotgun sequence.
ACCESSION   DAOQGS010000587 DAOQGS010000000
VERSION     DAOQGS010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24900_length_2606_cov_4.722070"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="nudC"
                     /locus_tag="PKZ52_16560"
     CDS             <1..696
                     /gene="nudC"
                     /locus_tag="PKZ52_16560"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNG61437.1"
                     /translation="GIASVLSPHPRAEDAELIYLGHWCGEPVWLQEDEVTSADTASLR
                     SLADILAPAEFALGSRAMQLLHWRQMHRFCGRCGTATEMSSTELCCHCPSCGASFYPR
                     ISPCVIGLVSRGDELLLASHHRHKVGRYSLLAGFIEAGESAEEAFAREVFEEVGIRIQ
                     QVRYVSSQPWPYPSQLMLGFFAEYQSGEIAPDASEILHAAWFHPERLPDIPSNVSIAG
                     KLIRAFLHERGFA"
     gene            1168..2232
                     /gene="sohB"
                     /locus_tag="PKZ52_16565"
     CDS             1168..2232
                     /gene="sohB"
                     /locus_tag="PKZ52_16565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HNG61438.1"
                     /translation="MEFLSNYGLFFAKTITWVVAFIVVISFMVAMGAKRSRPDKGSVT
                     VTKLNEEFEDLADALRVELLDEKAFKEEKKAEKKNRKKREKEASKAKKSVDASDESDK
                     KRVFVVDFDGDIKASAAEGLRHTVTSILSQARPQDEVVVRLESQGGMVHSYGFASSQL
                     ARIRARNIPLTICVDKVAASGGYMMACVANKILAAPFAVIGSIGVVASLPNFHRVLKK
                     NDIDYEMLTAGEYKRTLTVLGENTEKGRQKFQQDLEETHELFKEFIIANRPQLDINQV
                     ATGEVWFGSRALDSQLIDGLSTSDEYLTGLHPAADIFLIAYETKKTLAQKMGFSSQVF
                     ADGLFNALFERLSNRYWARN"
     gene            complement(2425..>2606)
                     /locus_tag="PKZ52_16570"
     CDS             complement(2425..>2606)
                     /locus_tag="PKZ52_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61439.1"
                     /translation="PDHPALDKSGQFDRSYTYHYKKNDWLKYATLGLLSKEETQGFDS
                     QDIYNKQDVKQPPNL"
BASE COUNT          609 a          614 c          705 g          678 t
ORIGIN      
        1 ggcattgcta gtgtgttgag cccccatcca cgcgcagagg atgccgagtt gatctatctg
       61 ggccattggt gcggtgaacc tgtatggttg caggaggatg aggttacttc tgcggacacc
      121 gcaagcctgc gcagcctggc cgatatattg gctcccgcag aatttgccct gggctcccgc
      181 gccatgcaac tgttgcactg gcggcaaatg caccgctttt gtggccgctg tggcacggca
      241 actgaaatgt ccagcactga gctgtgctgc cactgcccca gctgcggcgc gagtttctac
      301 ccgcgcatct ccccctgtgt aatcggtttg gtcagccgtg gtgatgagtt gttactggcc
      361 agccatcacc gccacaaagt gggccgctat tccctgttgg ccggttttat tgaggctggt
      421 gaaagtgctg aagaggcctt tgcccgcgag gtgtttgagg aggtcggcat tcgtatccag
      481 caggttcgtt atgtctccag ccagccctgg ccctatccca gtcagctgat gctagggttt
      541 ttcgccgaat accaaagtgg cgagatagca ccggatgcca gcgaaatcct ccacgcggct
      601 tggtttcacc ccgagcgttt gccggatatc ccctcgaatg tttccattgc cggtaaattg
      661 atccgcgctt ttttacatga acgcgggttc gcttagctgt attagctcag cccctgaagg
      721 ttgggccttt gccgttaaag tcttgtaact attctcagca ccctttgctg tagttggacg
      781 tgattctcgt gccaaataga aatcaccagg cgaatgcgtg ggtgttgtga gccctaagtc
      841 attccgccgc agtaataaat acaggttatt gcagacacgc cgtgaatact tccgtgtagg
      901 ctcgtcaaaa aggtccctct ttttgacggt ctgcaataac ctgtattcat caccactgta
      961 ccgcgaatcg attctgccga ctggctaaat ctgccttaat ttgccgcccg cgcttaagag
     1021 ggtaataagg tgtgctgaat agttacaaag tctttagaat gccccacgca cccccactga
     1081 tgtattggct tttacggcca tggtgcggcg tgtagcgtgg caggtgttga tctgcacaga
     1141 aattaatttt gtttgaggag cgatggcttg gaatttttat ccaattacgg gttgtttttt
     1201 gccaaaacaa ttacctgggt ggtggcgttt attgtggtga tttcgtttat ggtggccatg
     1261 ggcgctaaac gcagccggcc cgataaaggc agtgtaacgg tgaccaaact caatgaagag
     1321 tttgaggatt tggccgacgc attgcgggtt gaattattgg acgaaaaagc ttttaaagaa
     1381 gaaaaaaagg cagaaaagaa aaaccgcaaa aagcgggaaa aagaagccag caaagcgaaa
     1441 aaatcggtag acgccagtga cgagagcgat aaaaaacggg tgtttgtggt ggattttgac
     1501 ggcgatatta aagcctcggc cgctgaaggc ctgcgccaca cagtcaccag tattttatcc
     1561 caagcccgcc cccaggatga ggtggtggtg cggttagaaa gccaaggcgg catggtgcac
     1621 agctatggat tcgcctccag ccagttagcg cgtatccgcg cccgcaatat tcccctcact
     1681 atttgcgttg ataaggtggc ggcgagcggc ggttatatga tggcctgtgt ggccaataaa
     1741 attcttgccg cgccttttgc ggttataggc tcaattggcg tggtggcgag cctgccgaat
     1801 tttcatcgcg tgttgaagaa aaacgatatc gactatgaaa tgctcaccgc cggtgaatat
     1861 aaacgcactc taactgtatt gggtgaaaac accgaaaaag gccgccaaaa attccaacag
     1921 gatttagaag aaacccacga gttatttaag gaatttatta ttgccaatag gccgcaattg
     1981 gatattaacc aagtggcaac cggtgaagtt tggtttggca gccgcgctct ggatagccaa
     2041 ttaatcgatg gcttgagcac cagtgatgaa tatctgacgg gtttacaccc tgcggcggat
     2101 attttcttga tcgcttatga gacgaaaaag acgctggccc agaagatggg attttccagc
     2161 caagtgtttg cggatggatt gtttaatgcg ctgtttgagc gtctatctaa tcgctattgg
     2221 gcgcgcaatt aaaccggaac actacagggc ttattaaccc ggcagataaa tagatcgtcc
     2281 tgatctattt atccttcgca gcgctaaaac ctttagtgca gctcgcggcg gtttgcaccg
     2341 ccgagtgcgg catccatgcc gccattatta acccggcact aattgccgcc gggttaataa
     2401 aaccctgtag tgtttaagcg cagtttataa attgggcggt tgtttaacgt cttgtttgtt
     2461 gtaaatatcc tgggagtcaa agccttgggt ttcttctttg ctgagtaggc cgagggtggc
     2521 gtatttcagc cagtcatttt tcttatagtg ataggtataa ctacgatcga actggccgct
     2581 tttatccagg gccgggtgat caggaa
//
LOCUS       DAOQGS010000588         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24901_length_2606_cov_4.639357, whole genome shotgun sequence.
ACCESSION   DAOQGS010000588 DAOQGS010000000
VERSION     DAOQGS010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24901_length_2606_cov_4.639357"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1121
                     /locus_tag="PKZ52_16575"
     CDS             165..1121
                     /locus_tag="PKZ52_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG61440.1"
                     /translation="MKSVLTSERDVLYSHPIEEFANAAQLSRGEKIRLMYRNLVKTVR
                     EAPAEPGVQRMLDLGCALLQARSAHLIKPLETSGQLEYQDSLSAADENEIALEFTHPN
                     GETTPSERIVVLDLARHSLRINGRTPTAAVMITPNACDQLLLCFCGDAKSTASAEALE
                     ILELLAEGVACMIDLQRNHAQRRISDQGLGAGGIVKTLEEYQQSAALPEVYGVPARVL
                     EVLQRRVGRLGLSIGEVADDLNLSKRTLQRRLQQHNINFADLRDQVRFHHAISFLIHQ
                     ELSIDSISTALDFSDRTSFTNAFKRWTGLSPSTFRKLFRDYV"
     gene            1251..1583
                     /gene="yajC"
                     /locus_tag="PKZ52_16580"
     CDS             1251..1583
                     /gene="yajC"
                     /locus_tag="PKZ52_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNG61441.1"
                     /translation="MSFFIASAQAADAAAAAAPNPILQILPFVGIFVLMYFFMIRPQR
                     KRQKEHQELLSALSKGDEVVLNSGMLGKIVDVDDAYLTLDVADNVKMKFQKHAVVAVL
                     PKGTIKAI"
     gene            1717..2361
                     /gene="ccmA"
                     /locus_tag="PKZ52_16585"
     CDS             1717..2361
                     /gene="ccmA"
                     /locus_tag="PKZ52_16585"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNG61442.1"
                     /translation="MSLLRFSEFTCWRSDEALFKPVNFSLDSGDIVQVVGPNGAGKTT
                     LLRSLCGLYNHYQGDILWHERSWQSQFYDFSQNLLYLGHTPGVKKSLTAAENLLWYTG
                     IQQGRSPQQQVEHALAQVGLADYADTLCQEMSAGQLRRVALARLYISQRPLWVLDEPF
                     TAIDVLGVSQLEQQLQHHVQRGGTVLLTTHQPLAIAQVKNLTLEACRHEASYES"
     gene            2351..>2606
                     /locus_tag="PKZ52_16590"
     CDS             2351..>2606
                     /locus_tag="PKZ52_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNG61443.1"
                     /translation="MSLETYSLSHALAASLKRELLLAVRQKSELFNPLLFFLVVVVFV
                     PLGITPDKNFLAKLAPGMIWVFALLATLLSLESLFKADFED"
BASE COUNT          654 a          685 c          634 g          633 t
ORIGIN      
        1 gtagtttcgg taatttatgg cattcaattc aacggcgtgg cacatgggaa gctggtttgc
       61 ctcgggaata agaactagtt tgtccaccct aaaattcagc aatgctcgct cttttaataa
      121 cagtttgtta taaatattgc ggcctttctt aaatggtgaa ctgtttgaaa tctgtactca
      181 ccagtgaacg tgacgtgctg tactcacacc cgatagaaga attcgcgaac gcagcgcagc
      241 tgagccgcgg cgaaaaaatt cggttgatgt accgcaatct ggtcaaaacg gtgcgcgagg
      301 caccggctga acccggtgta cagcggatgc tggatttagg ttgcgcgctg ttgcaggccc
      361 gctctgccca ccttataaaa cccttggaga caagcggcca gctggagtat caggattcac
      421 taagtgccgc agacgagaac gaaatcgcgc tagaattcac gcatcccaac ggagagacaa
      481 ccccctccga gcgcattgtt gtgttggatt tggcccgcca ttcgctgcgc atcaacggac
      541 ggacgcccac cgcagcggtc atgatcacac cgaatgcctg cgatcagttg ctgctgtgct
      601 tctgcggcga tgctaaatcc accgccagtg ccgaggcgtt ggaaattctc gaactattgg
      661 cagaaggtgt cgcatgcatg atcgacctgc agcgcaacca tgcgcagcgt cgtattagcg
      721 atcaaggcct cggggccggt ggaatagtca aaacccttga agagtatcag caatcagctg
      781 ctctacctga ggtctacgga gtgcctgcac gggtgctgga agtcttacag agaagagtcg
      841 ggcgactagg cctgagcata ggcgaagttg ccgacgactt aaatttgtct aagcgcaccc
      901 tgcagcgacg cctgcaacaa cacaacatca attttgcgga tctacgcgac caagtgcgct
      961 ttcaccacgc catcagtttt ctaatccatc aagaactcag tatcgatagc atatccacag
     1021 ccttggattt ttctgatcga actagcttca ccaatgcgtt taaacgctgg accgggctct
     1081 cgcctagcac cttccgcaaa ctgttccgcg actacgtcta gggcggcggc gcttaaacct
     1141 aaaaaccaca ggtgaaacgc cctagccttc ctaacagcta tttataatgt tgctcctgca
     1201 aggcaaaact tgtagagtcg gcagccccaa aaagttgttg gaattaccct atgtcttttt
     1261 ttattgccag tgcccaagcc gccgatgctg cagcggccgc tgcccccaac cccattttgc
     1321 aaatactccc cttcgtggga atatttgtgt tgatgtattt ctttatgatc cgcccccagc
     1381 gcaaacggca aaaagagcat caagaactac tgagcgccct aagtaagggt gacgaagtgg
     1441 tacttaacag cggcatgctg ggcaaaattg tcgatgtgga cgacgcctat ttaaccctag
     1501 atgtagccga caacgtgaag atgaagtttc aaaaacacgc tgtggtggca gtgcttccca
     1561 agggcacaat caaagcgatt taatcgcctc ttaagtaaat aatccgtacc atctccctgc
     1621 agggctatta gcctgaaatt ttagccctgc acctacgtct gcagcagaga ctgggcagag
     1681 gttagtagca atttaattgg ccgatgagaa caccgcgtga gtctactgcg atttagtgaa
     1741 tttacctgtt ggcgcagtga tgaagctctg tttaaaccgg tcaatttttc cctcgatagt
     1801 ggtgatattg ttcaggttgt aggccctaac ggcgccggga aaaccactct actgcgcagt
     1861 ctttgcggct tatataacca ctaccagggt gacatcctct ggcacgagcg ctcctggcag
     1921 agccagtttt atgatttttc acaaaacctt ttgtatctcg gccatacccc aggcgttaaa
     1981 aaatccctta ctgcggcgga aaacctgctc tggtatacag gcattcaaca aggtagatcg
     2041 ccccagcagc aagtagaaca cgcacttgcc caagttggcc tagcagatta tgccgacacc
     2101 ctgtgccaag agatgtcggc agggcaattg cggcgcgttg cactggcgcg cttgtatatc
     2161 tcacaaaggc ccctatgggt actcgatgaa cccttcaccg ccatagatgt actcggcgtt
     2221 agccagttag aacagcagtt acaacatcac gtacaacgcg gtggtacggt gttactgacc
     2281 acccaccagc ccctcgccat tgcacaggta aaaaatttga ccttagaagc ctgtcgacat
     2341 gaggcctctt atgagtcttg aaacctacag cctcagccac gcccttgcag ctagtttaaa
     2401 acgcgaactc ttattggccg tacggcaaaa aagtgagcta tttaatccac tgttattttt
     2461 tttggtggtg gtggtatttg taccgctggg gattaccccc gacaaaaatt ttctcgccaa
     2521 actcgcaccg ggtatgattt gggtattcgc cttattggca actttgttgt cactggagag
     2581 tctcttcaaa gccgattttg aagatg
//
LOCUS       DAOQGS010000589         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24941_length_2604_cov_3.850530, whole genome shotgun sequence.
ACCESSION   DAOQGS010000589 DAOQGS010000000
VERSION     DAOQGS010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24941_length_2604_cov_3.850530"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2604
                     /locus_tag="PKZ52_16595"
     CDS             <1..>2604
                     /locus_tag="PKZ52_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNG61444.1"
                     /translation="SIHFATPNPALALDASPFVVTEAFTPWPIAASERLAGVSSFGIG
                     GTNAHAVLQGVDYELADYEGPEYIPFCLSAKNPEGLARLAATYGRYLEQNPDKNTAAL
                     AAYLLKHRTPFPHRLSLGAANATGLSHALQEASFEDTGFKADTCILAFPGQGSQVLGM
                     GRELMAKVPAFAQALETCFELLLEQGTDLAEVMWGEDKSLLNQTQYTQLALFVIGYGL
                     SQSLKELGVSLQGAIGHSIGELVAATCAGVFSLPVALKVVATRGRLMQAQEAGAMLAV
                     RRPAPEVEPLLPEGAVIAAVNTADSCTLAGTFAAIDAAEAALKAAGMKTKRLETSHAF
                     HSPSMDAAAREFARTLADVELNPPALKFISCVTGQWITADQAQSIDYWASQLRQPVRF
                     SDGLKALAELPNLVVIEAGPGRTVCGMVLADLVERSDVRMVPLLAQTGEQEVNGFSQA
                     LGSLWQYHVPLVWPDLPITPARFSRLPPYPFAHRIHEITAQPKPVALVQAAEHKDYSD
                     PVATVSASAPTEQPTAFVSTSPIQSEQDLMKDALIPQLETLFSDISGMDLHGADRKSS
                     FFELGLDSLLLTQSAIKLKKTYKVGVSFRQLLNELDHLDALANYLIEQKAEGLPSVAA
                     PVPGPVAQVQAAAPGAPAVAAAAPLAAAVFAPQPSNFAPINAIPVAADGSMLSLVQQQ
                     LNLMQQQLQLLTQGGAAPLAPAAVLGALSAAPVPVASASAPATKAAGAVKPFGAGTRI
                     NVKRSNEMTEAQAKHLAALSEAYNSQFKSSKAYAQTHRPYLADPRVVSGFRTSLKEII
                     YPIVVARSEGAYLWDIDGFRFVDITCGFGSNFFGNGAPFIKDAVAQQLQTGYEIGPQH
                     PVEEPRQQRDLP"
BASE COUNT          518 a          821 c          738 g          527 t
ORIGIN      
        1 cagcattcac tttgctaccc ccaacccggc cttagccctc gacgccagcc catttgtggt
       61 caccgaagcc ttcaccccct ggcctatagc cgcctctgag cgcttggcgg gggtgagttc
      121 gtttggtatc ggcggcacca acgcccatgc ggtgttgcaa ggggtggatt atgagctggc
      181 cgattatgaa gggcctgagt acatcccctt ctgtttgagc gctaaaaacc ctgaaggcct
      241 tgcgcgcctg gccgccacct acggccgcta cctggagcaa aacccggata aaaacactgc
      301 ggccctggcg gcctacctgc taaaacaccg cacgccattt ccccacaggt taagcctggg
      361 ggcggccaat gccaccggct tatctcatgc tctgcaggaa gcgagctttg aggacaccgg
      421 cttcaaagcc gatacctgca ttctggcctt ccccggccag ggcagccagg tgttgggtat
      481 gggccgggaa ttgatggcca aggtgcccgc atttgcccaa gccctggaga cctgttttga
      541 actcttgcta gagcagggca ctgacttagc tgaggttatg tggggggagg ataagtccct
      601 gctcaaccaa acccagtaca cccagctggc gctgtttgtc atcggctatg gcctcagcca
      661 aagccttaag gagctgggcg taagcctgca aggcgccatc ggccacagca tcggcgagct
      721 ggtggcggcc acctgcgccg gtgtgtttag tctgccggtg gccttaaaag tggtggcgac
      781 ccgcgggcgg ctgatgcagg ctcaggaggc cggtgccatg ctcgccgtgc gcaggcccgc
      841 acctgaggtt gagccgttgc tgccggaagg cgcggtaatt gccgccgtaa ataccgccga
      901 tagctgcacc ctggcgggca cctttgccgc tattgatgcc gccgaggcgg ccttgaaagc
      961 agcgggcatg aaaaccaaac ggctggagac ctcccacgcc ttccattccc cctccatgga
     1021 tgcagcagct agagagttcg cccggaccct ggccgatgta gagttaaacc caccggcctt
     1081 aaagtttatc tcctgtgtca ccggccagtg gatcaccgct gatcaggcgc aaagcatcga
     1141 ctattgggcc agccaactgc gccaaccagt gcgctttagc gatgggctta aagccctggc
     1201 agagctgccc aacttagtgg tgattgaagc cgggccaggc cgcactgtgt gtggcatggt
     1261 gctggctgat ctggtcgagc gtagcgatgt gcgtatggtg cctttgcttg cccaaaccgg
     1321 cgagcaagag gttaatggct tcagccaagc cttaggctca ctctggcagt accatgtgcc
     1381 gctggtttgg ccggatttgc cgattactcc ggcgcgcttt agccgcttac cgccttaccc
     1441 ctttgcccat cgcatccacg aaatcaccgc ccagcccaaa cctgtggcac tggtgcaggc
     1501 ggctgaacac aaagactatt cagacccagt cgctactgtg agcgcctctg cgcctactga
     1561 gcaacccacc gcattcgtta gtacctcacc cattcaaagc gagcaggacc ttatgaaaga
     1621 cgccctaatc cctcaactag agactttgtt ttccgatatt tcaggcatgg acctgcacgg
     1681 cgcggatcgt aagtccagct tctttgaact ggggctggac tcactcttgc tcacccaatc
     1741 ggctattaag cttaagaaaa cctacaaagt gggggtgagt tttcgccagt tgcttaacga
     1801 gctggaccac ctagatgccc tggccaatta cttgatcgaa cagaaagcgg agggcctgcc
     1861 cagtgtggca gcgccagtgc ccggcccggt tgcccaagtg caagcggctg ccccaggggc
     1921 gcctgctgta gcagccgcag cacccctggc ggctgcagtt tttgcccctc agcccagcaa
     1981 tttcgcaccc ataaatgcaa taccggttgc cgccgatggc agtatgctga gcctggtaca
     2041 acagcagctt aacctgatgc agcagcagct gcagttgctc acccagggtg gagctgcacc
     2101 cttggcgcct gctgcggtgc taggcgccct gagcgctgcg cccgtacctg ttgcgtctgc
     2161 cagtgcgccc gccaccaaag ccgccggtgc cgtcaaaccc tttggtgcag gcacccgcat
     2221 caatgttaag cgcagcaatg aaatgaccga ggcccaggct aaacacctgg cggctctgtc
     2281 ggaggcttac aacagccaat ttaaatcctc caaagcctat gcccaaaccc accggccata
     2341 tcttgcggac cctcgggtgg tgtccggctt tcgcaccagc ctaaaagaga tcatctaccc
     2401 catagtggtg gcccgctcgg agggcgccta cctgtgggat atagacggct tccgctttgt
     2461 ggatatcacc tgtggctttg gctccaactt ctttggcaac ggcgcgccct ttatcaagga
     2521 tgccgtggcc cagcagctgc aaaccggtta tgaaatcggc ccccagcacc cggttgaaga
     2581 gccgcgtcag cagcgggatc tgcc
//
LOCUS       DAOQGS010000590         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24977_length_2602_cov_4.420102, whole genome shotgun sequence.
ACCESSION   DAOQGS010000590 DAOQGS010000000
VERSION     DAOQGS010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24977_length_2602_cov_4.420102"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKZ52_16600"
     CDS             complement(<1..676)
                     /locus_tag="PKZ52_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG61445.1"
                     /translation="MAEFILEALNISKAFGGVAALDDVSLKLKHHSFHALLGENGAGK
                     STLVKCIMGYYQPDSGQLLFNNRQEVMSSPQVAAAKGIGMVYQHFTLIPNMSIAENIL
                     LSRPNVPLVIPWKSAIDELTAKMHGMPFQFDLNRYVRDLSVGEKQKVEITKQLLLDSK
                     ILILDEPTSVLTPTEADEVLGKVYALTQAGKLSVLMISHKFREVMQYADTVTVLRKGK
                     YVGTVAT"
     gene            complement(676..1356)
                     /locus_tag="PKZ52_16605"
     CDS             complement(676..1356)
                     /locus_tag="PKZ52_16605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNG61446.1"
                     /translation="MYIPATPYPWPYNGDITPANTALIIIDMQIDFCGPGGYVASMGY
                     DISLTRAPIEPIKKLLALMRAKGFYIIHTREGHRPDLADLPPNKRWRSRQMGAGIGDA
                     GPCGRILVRGEPGWNIIEELAPLPGEIIIDKPGKGSFCATDLDLILRTQGMRNLIITG
                     ITTDVCVSTTMREANDRGYECLMVEDCCAATDPANHLAAINMVKMQGGVFGATTGSAQ
                     LLAQLEQL"
     gene            complement(1381..2304)
                     /locus_tag="PKZ52_16610"
     CDS             complement(1381..2304)
                     /locus_tag="PKZ52_16610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61447.1"
                     /translation="MDASDNSLWVLLVAVIGGAIRVGTPFIFVSLGECLTEKSGRINL
                     GLEGNLIMGAMSAYAVSYLTGSPWLGVLAAAGSGCILGAVHALLCQHPKVNDIALGIA
                     MMLFATGLAFFFGKPFIQPSAPHLPSVSLGAWSDWPVLKSALDINLLFFVGVALAPFI
                     AWFLKSTRPGNRVRIAGESSDAALAMGLSVNRLRFFSTVVGGAMASSVGGSYLSLFYP
                     GSWSEGLSSGQGLMAVALVIFARWDPIKCLYASFLFGSAAALGPALQSVGVTSGYYLF
                     NAAPYVLTLIIMIATSNNTGTIKGMPGEFVK"
     gene            complement(2335..>2602)
                     /locus_tag="PKZ52_16615"
     CDS             complement(2335..>2602)
                     /locus_tag="PKZ52_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61448.1"
                     /translation="GRANSSLAAGYGYAGILVAFLARQNPLAVIPYAILLGGIQAAGG
                     LLQRNHDLPDATTGVIQGIIFMVILTSEIFYGRFAWLQSKPRAA"
BASE COUNT          739 a          685 c          594 g          584 t
ORIGIN      
        1 aggtggcaac ggttcccaca tatttaccct tgcgcaaaac agtcaccgta tcggcgtact
       61 gcatcacttc gcggaattta tggctaatca taagtacgct taacttcccc gcctgagtga
      121 gcgcataaac cttgcccagc acttcatccg cttcagtggg ggttaacact gaggtaggct
      181 catcgagaat aagaattttt gaatccaaca ataattgttt ggtgatttca actttttgtt
      241 tttcaccgac ggacaaatcc cgcacatagc ggtttaaatc aaactgaaac ggcatgccgt
      301 gcatttttgc cgttaattca tctatggcag atttccaggg gattacaagc ggcacattcg
      361 ggcgcgagag cagtatgttt tctgcaatgg acatattagg gatcagggta aaatgctgat
      421 acaccatgcc tatgccttta gccgccgcaa cttgcgggga ggacatcacc tcttgtctat
      481 tattaaacaa taattggccg gagtcgggct gatagtagcc cataatacat tttactagtg
      541 tgcttttgcc cgccccattt tcacctaata atgcgtgaaa gctgtggtgt tttaatttta
      601 ggcttacatc atccagcgcg gccacaccac caaaggcttt gctgatattt aatgcctcta
      661 aaataaattc ggccattaca gctgctccag ctgggccaat aattgcgcgc tgcccgtggt
      721 ggcaccaaat acaccgcctt gcatttttac catattaata gccgccaggt ggttagccgg
      781 gtccgtcgcg gcgcagcagt cctccaccat aaggcattca taaccgcgat cgttggcctc
      841 gcgcatggtg gtgctcacac acacatcggt ggtgataccg gtaataatta aattgcgcat
      901 accctgggtg cgcagaatta aatctaaatc cgtcgcgcaa aatgagcctt tgccgggttt
      961 atcaataatt atctccccag gtaagggggc taattcttca ataatattcc agccgggctc
     1021 gcctcttact aatatgcgcc cgcaggggcc agcatcgcca atacctgcgc ccatttggcg
     1081 gctgcgccag cgtttattag gcggtaaatc cgctaaatcc ggccggtggc cttcgcgggt
     1141 gtgaataata taaaaacctt tagcgcgcat aagagctaag agttttttaa tcggctcgat
     1201 gggtgcgcgg gttaaactga tgtcataacc catggaagcc acataaccgc cggggccgca
     1261 aaaatcaatt tgcatatcga taataattaa agcggtatta gcgggcgtaa tatcgccgtt
     1321 atagggccat gggtaaggcg ttgctggaat atacataaaa cagtcactcc aaaacttagg
     1381 ttatttgaca aattcccccg gcataccttt gatagtgcca gtattattgg aggtagcaat
     1441 cataatgatc agagttaaca catagggcgc ggcattaaat aaataatacc cggaggttac
     1501 acccaccgat tgcaaggccg ggccgagggc cgccgcgctg ccgaataaaa aactcgcgta
     1561 caggcattta attgggtccc agcgggcaaa aataaccaaa gccactgcca ttaaaccctg
     1621 cccactggaa agcccctcac tccaactgcc ggggtaaaac agcgataaat aactgccgcc
     1681 caccgacgac gccatagcgc cgcccaccac agtgctaaaa aaacgcaggc gattaaccga
     1741 tagccccata gccaaggcag catcagaact ttctccggcg atgcgcaccc ggttgcccgg
     1801 ccgagtggat tttaaaaacc aggcaataaa aggggccaaa gccacgccaa caaaaaataa
     1861 taaattaata tccaaggcag attttaaaac cggccaatcg ctccaagcgc ctaagcttac
     1921 cgatggcaaa tgcggcgcac taggttgaat aaagggtttg ccgaagaaaa atgccaggcc
     1981 cgtggcaaat aacatcatgg caataccgag ggctatatca ttcaccttag ggtgttggca
     2041 taacagtgca tgtaccgcac ctaaaataca gccgctgcca gcggcagcga gcacacctaa
     2101 ccagggcgaa ccggttaaat aagaaactgc ataggcactc atagccccca taattaaatt
     2161 gccttccaac cccaaattaa tgcggccgga tttttccgtt aaacactcgc cgaggctgac
     2221 aaaaataaag ggcgtgccga cgcgaatggc gccgccaatc accgccacca atagcaccca
     2281 caggctgtta tcacttgcgt ccattaatca cctcttaaaa atcaacacgc gcaactatgc
     2341 agcgcgaggt ttggattgca accaggcaaa gcggccataa aaaatttcgc tggtaagtat
     2401 caccataaaa ataatgcctt gaatcacgcc ggtagtggca tcgggcaaat cgtgattgcg
     2461 ctgcaataaa ccgccagccg cttgaatacc gcccaataaa atggcgtagg gaataaccgc
     2521 taagggattt tgccgggcta aaaacgccac caaaattcca gcatacccat aacctgccgc
     2581 gagggaagaa ttggcccgcc ct
//
LOCUS       DAOQGS010000591         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24978_length_2602_cov_4.110718, whole genome shotgun sequence.
ACCESSION   DAOQGS010000591 DAOQGS010000000
VERSION     DAOQGS010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24978_length_2602_cov_4.110718"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKZ52_16620"
     CDS             complement(<1..429)
                     /locus_tag="PKZ52_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNG61449.1"
                     /translation="MSKVLIIGAGGVGGVVTHKCAQLADTFSEIILASRTESKCKAIA
                     AQLKRPIRTAQVDADNVAELTALLEKEKPNLVINVALPYQDLHIMDACLNAGVDYLDT
                     ANYEPPNVAKFEYSWQWAYQDKFKNAGLMALLGSGFDPGVT"
     gene            complement(457..2373)
                     /gene="speA"
                     /locus_tag="PKZ52_16625"
     CDS             complement(457..2373)
                     /gene="speA"
                     /locus_tag="PKZ52_16625"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNG61450.1"
                     /translation="MNKLVHHAWTAAHSADLYGVNDWGAGYFGVSAKGDITVTAHDLK
                     DDSSVSLLDIVNEIRARGMDTPVLVRFENLLAQQITQLNEAFRAAIRNSGYLGEYRGV
                     FPVKVNQQSHVIEEIAQVGAKYGHGLEAGSKPELIIALAHLESRDACIVCNGYKDEEF
                     IDLGLQACKMGYRVFFVVETPSELPIILERSRALGIRPLIGVRVKLASIVGGHWNATS
                     GDRSIFGLSIAQVITLVDDLKRENMLDCLQLLHYHLGSQIPNIRDIRTGVIEACQVYV
                     DLVREGAAMGYLDLGGGLAVDYDGSKTNYMHSRNYTLEEYCADIVDVVMTTLDPHEIA
                     HPTIVTESGRATVAYSSVLVFNVLDVATFEPGPLPEVIGEQENQMIHNLKDLVTSATM
                     RNLQECYNDALFYRDEIRELFKRGQVNLRSRSLAENLFLKVMHNLLALAEDAPKGFKE
                     IHKLKEQLSDIYYCNFSLFQSLPDVWAIDQIFPVIPIHRLDEKPTRDAIIADITCDCD
                     GKIDKFVDQLGSRRTLPLHDLKDNEEYYIGAFLVGAYQETLGDLHNLFGDTNVVSVRL
                     TEGGTYEFVHEIEGDSIADVLSYVEYHPKEMQERFRRAAELAVREGRITAAERLKIMN
                     AFSASMRGYTYYEK"
BASE COUNT          600 a          615 c          730 g          657 t
ORIGIN      
        1 ggttacacct gggtcaaagc cggaacctaa taatgccatt aagcctgcat ttttgaattt
       61 atcctgatag gcccactgcc agctgtattc aaatttagcc acattgggtg gctcgtagtt
      121 ggcggtatca agataatcca caccggcatt taaacaggca tccatgatgt gtaagtcctg
      181 gtagggcagg gctacgttaa tcaccaaatt gggtttttct ttttccaaca gggcggttaa
      241 ttccgccacg ttgtccgcat ccacctgggc ggtgcgaatg gggcgtttta attgcgcggc
      301 tatggctttg catttagatt cggtgcggct ggcgagaata atttcgctga aggtgtcggc
      361 cagttgggcg catttgtggg tgactacgcc gcctacgccg cctgcaccaa taattaatac
      421 tttgctcatc aatatttcct ccgaaggaac tgggggttat ttttcgtaat aggtgtagcc
      481 gcgcatgctg gcggaaaatg cattcataat ttttaagcgc tcggcagcgg taatgcggcc
      541 ttcgcgcacg gcgagttccg ctgcgcggcg gaagcgctct tgcatttctt tagggtggta
      601 ttccacataa cttaaaacat cggcaatgga atcgccctca atttcatgca caaattcgta
      661 ggtgccgcct tcggttaagc gcacgctcac cacgttggta tcaccaaata aattgtgtaa
      721 atcccctaaa gtttcctggt aggcgcccac taaaaaggca cctatatagt attcttcgtt
      781 gtcttttaaa tcgtgcaggg gcagggtacg gcgggagcct aattgatcga cgaatttatc
      841 aatcttgccg tcgcagtcgc aggtgatgtc ggcaatgatg gcatcgcggg tgggcttttc
      901 atccaggcgg tgaatgggaa taaccgggaa gatttggtca atcgcccaca catccggcag
      961 ggattggaac aaactaaaat tgcagtaata aatatccgat aactgctctt ttaatttatg
     1021 gatttcttta aatcctttag gggcatcttc ggccaaggcc aataaattgt gcatcacctt
     1081 gaggaataaa ttctccgcca aggagcgcga gcgcaggttt acttggccgc gtttaaataa
     1141 ttcgcggatt tcatcgcggt agaagagggc atcgttataa cactcttgca ggttgcgcat
     1201 ggtggcggag gtaaccaaat cctttaaatt gtggatcatt tggttttcct gctcgccgat
     1261 tacttccggc aaggggccgg gttcaaaggt ggccacatcc aatacgttaa acaccagtac
     1321 tgaagaataa gccacggtgg cgcggccgct ttcggtgact atggtggggt gggcaatttc
     1381 gtgggggtct agggtggtca tgaccacgtc cactatatcc gcgcagtatt cttctaaggt
     1441 gtagttgcgc gagtgcatat agttggtttt ggagccgtcg tagtccaccg ccaagccgcc
     1501 acctaagtct aaataaccca tggcggcgcc ttcgcgcact aagtctacat acacttggca
     1561 ggcttcaatt acaccggtgc ggatatcgcg aatattgggg atttgtgagc ccaggtggta
     1621 gtgcaataac tgcaggcaat cgagcatgtt ttcgcgtttg aggtcatcca ccagggtaat
     1681 tacctgggcg atggataaac caaaaatgga gcggtcgccg ctggtggcgt tccaatggcc
     1741 gcctacaata gaggcgagtt ttacgcgcac gccaatcaag gggcgaatgc cgagggcgcg
     1801 gctgcgctcg agaataatgg gtaattcgga gggagtttcc accacgaaaa atacccggta
     1861 acccatttta caggcttgca aacctaggtc gataaattct tcatctttgt agccgttgca
     1921 aacaatacag gcatcgcgag attctaaatg tgccagggcg atgatgagtt cgggtttgga
     1981 gccagcttcc aggccgtggc cgtatttggc gcctacctgg gcgatttctt caattacatg
     2041 gctttgttgg tttaccttga cggggaacac gccgcggtat tcacccaggt agccgctgtt
     2101 gcggatggcg gcgcggaagg cttcattgag ctgggtgatc tgctgggcca ggaggttctc
     2161 aaagcgcacc agtaccggtg tatccatgcc gcgcgcgcgg atttcgttga ctatatccag
     2221 gagggataca ctgctgtcat ctttaagatc gtgggcggtg actgtgatgt cgcccttggc
     2281 ggagacgcca aagtaaccgg ccccccaatc gttgacgccg tataaatcgg cagaatgagc
     2341 ggcagtccaa gcgtggtgca ccagtttatt caatgtcgga gttctcgcag cttagggtca
     2401 aaaaacccag gttaaagaag ggattttgcg gtttgatgac ggagccttga agacgacaaa
     2461 accccggtcg caaagggcgc gaactatcgc agattttgtg ggctaacgct agggcagagc
     2521 gatcagcgca gcctatggcg ggcatgggct gggggcgtaa ttcgccactg tcttaatact
     2581 gctggcgccg gggctatgag cg
//
LOCUS       DAOQGS010000592         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_24995_length_2601_cov_4.733700, whole genome shotgun sequence.
ACCESSION   DAOQGS010000592 DAOQGS010000000
VERSION     DAOQGS010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24995_length_2601_cov_4.733700"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1083
                     /locus_tag="PKZ52_16630"
     CDS             313..1083
                     /locus_tag="PKZ52_16630"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61451.1"
                     /translation="MRLVLAHGILGFGSDQFDFGINYFNAVRPIFERQGFEVFEPTVN
                     PLGSLAERSEQLATKLALHWPDESDIYIVAHSMGGLDARRVIARYQVGKRIKKLVTLA
                     TPHFGSPVADAVLGKNSALLGFIPNKIRTSLEKATGALNDLTTRDALQDESQDWVQYH
                     SIGCTIKNKGFFRNSQLFSLSQAIGKISDQPNDGVVPLKSAIGGNTHFSVWDGFDHND
                     AIGWSTGWFGFALIKNLLKMPSPHIQRYQELAAQLAHK"
     gene            complement(1280..>2601)
                     /locus_tag="PKZ52_16635"
     CDS             complement(1280..>2601)
                     /locus_tag="PKZ52_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNG61452.1"
                     /translation="GTHFQEMGAVEMARLVVEGLEDNKQRADMANIISRLMPRYTLTV
                     ASTVTNSGEFTRSNRYPAGTPITLKYLPDGTNKFTQWIQSGQVLANNALAQFTMPKND
                     VRIDAVVNNVPPRTSSSSSSSSSSSSSSGTPNFPAAKVFLIGDSTVSYYNSSYAPQEG
                     WGQEIGNFFNSAKITIDNRAIGGRSSKSFYNDHWAAVKADVKAGDFVFIQFGINDRNS
                     ADPARYAPTTDGTFQDYMTRFAKETQALGATPVFVSTVPRNAWNGTSVYNAYHEHPLV
                     TIDLAKSLKIPYLDLNAASTALMESAGEEFSTYFLYLNIPVGEFSTVTTAKADNVHFQ
                     AYGAQEMARLIASLIEAQKATAPMTQLAAALKPRVQVTITGRSDAGLVTQSNAYPAGF
                     KLMLRARPNSTHRFLRWQDAAGTTLSTQAIYTYTVPNSASSVTAVFN"
BASE COUNT          612 a          553 c          714 g          722 t
ORIGIN      
        1 tccgcaggag gggaatatta ggccgtgccc ctagaataat atccacacat cgatgattga
       61 atgatattaa tttaaaaatg ggtgttacgg gaattgactc tgttggatag ggcatgtttg
      121 agtcgcgtag ttaaattgct gggctttgta tggtttttta aggtgctgta cgggttatta
      181 ttggcgggct atatacttga caggtcggaa ggtgctgcgc ttttccggcc ttggtgttaa
      241 tcggcgttat attaaaagct ctggcggtta ggctaattgc caggtgacac ataaaaaacc
      301 tggagtttaa ttatgcggct tgtacttgcc catggcatat tgggttttgg tagcgatcag
      361 tttgattttg gtatcaatta ttttaatgct gtacggccaa tatttgagcg gcagggtttt
      421 gaggtgtttg agcccacggt taacccgctg gggtctttgg ctgagcgcag tgaacagctg
      481 gcaacaaaac tggctctgca ttggccggat gaaagcgata tttatatagt ggcccatagc
      541 atgggcggcc ttgatgcccg ccgtgtgatc gcccgttacc aggtgggtaa acgcattaaa
      601 aaattagtta ccctagcaac accacatttt ggctcgccgg tggctgatgc ggttttgggt
      661 aaaaactctg cgttattagg atttataccc aataaaattc gcacctctct tgaaaaggcc
      721 accggtgcct taaatgactt aaccacacgt gacgccttgc aggatgagtc gcaggattgg
      781 gtgcagtatc acagcatcgg ctgcacgatt aaaaataaag gcttctttag aaattcccag
      841 ttatttagcc tgtctcaggc tataggcaaa ataagcgacc aacctaatga cggggttgtg
      901 cccctgaaat cagcaatagg tggcaacact catttttcgg tttgggatgg ctttgatcat
      961 aacgatgcaa ttggttggtc caccggatgg tttggttttg ccttgattaa aaatctgctc
     1021 aagatgccat cgccacatat tcagcgctat caagaattgg cggcacagct tgctcataag
     1081 taaacgggcg ccgagaggaa tacagattat ttcggacagg ccgtgaatac gttcgtgtag
     1141 gcccgtcaaa aagcttattt gacggcctga ataatattta ttttcctatt tgcggtaagt
     1201 ctttgggccg gattttcaca ccattgcggg tatgaggcct caggctcagt tacaggacgc
     1261 atacagatgc gctgttcttt tagttaaaaa ccgccgttac actgctagcc gaattaggca
     1321 ccgtgtaggt gtaaatagcc tgtgtggaca gggttgtgcc tgcggcatcc tgccagcgca
     1381 gaaagcgatg ggtgctgtta gggcgggcgc gcagcatgag tttaaagcct gcagggtagg
     1441 cgtttgattg tgttaccagg ccagcatcac tgcgaccggt aatagtcacc tgcacgcggg
     1501 gtttaagcgc tgcagccaat tgcgtcatgg gcgcggtggc tttttgcgct tcaattaaac
     1561 tggcaattaa gcgcgccatt tcttgggcac cataggcttg gaaatgcaca ttatcggcct
     1621 tggccgttgt gacggtagaa aattcaccga caggaatatt taaatataaa aaataggtgg
     1681 aaaattcttc cccggcgctt tccattaatg cggtgctggc ggcgtttaaa tctaaataag
     1741 gaatttttag gcttttggct aaatcaatcg tcaccagtgg gtgttcgtga taggcgttgt
     1801 atacgctggt gccgttccag gcgttgcgcg gtacggtgga tacaaatacc ggcgtggcgc
     1861 caagggcttg ggtttctttg gcaaaacgcg tcatataatc ctgaaaggtg ccgtcggtag
     1921 tgggcgcgta acgggcgggg tcggcgctgt tgcgatcatt aatgccaaat tgaataaaaa
     1981 caaaatcacc tgcttttaca tccgctttta ccgcggccca atgatcattg taaaaacttt
     2041 tcgagctgcg cccgccaatg gcgcggttgt cgatggttat tttggcgcta ttaaaaaagt
     2101 tgccaatctc ctggccccag ccttcttggg gcgcatagct gctgttgtag taggaaacag
     2161 tggagtcgcc aattaaaaat actttggccg cggggaaatt tggggtgccg gaactggatg
     2221 acgagcttga gcttgacgat gagcttgagc tggtgcgcgg cggtacatta tttaccaccg
     2281 cgtcgatgcg cacatcgttt ttgggcatag taaattgagc caaggcgtta ttggccagca
     2341 cctggccgct ttgaatccac tgggtaaatt tatttgtgcc gtctggcagg tattttagcg
     2401 tgatgggtgt gccagccggg tagcggttag agcgggtaaa ttcgccgcta ttagtaacgg
     2461 tgctggcgac ggttaatgtg tagcgcggca ttaaacggct aataatattc gccatatcgg
     2521 cgcgctgttt attatcttct aaaccttcta ccaccaggcg ggccatttcc accgcaccca
     2581 tttcttgaaa gtgggtgccg t
//
LOCUS       DAOQGS010000593         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_25046_length_2598_cov_4.758553, whole genome shotgun sequence.
ACCESSION   DAOQGS010000593 DAOQGS010000000
VERSION     DAOQGS010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25046_length_2598_cov_4.758553"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..>2598
                     /locus_tag="PKZ52_16640"
     CDS             245..>2598
                     /locus_tag="PKZ52_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61453.1"
                     /translation="MEIASNGQPVPKVFSAGGALNIKSKQIEISGALSAPFGQVNLTG
                     DDVHLAKSALVNVSGDNQLIPFGSIIGNDLDWLYSLSPLANTTLIDNTPLKQVNIKAE
                     RILLDEGSRINLAGGGDLLGYQFAPGPTGSKDVLAISSPTEGFALLPAGNFVAYDSAL
                     QGQDGIATGTRLTLSNNPLLPAGDYYLLPSRYALLPGAYWVKPTGKLQAPGSSFTQPD
                     GGITLTGQLGKAHTPIHSADWQGFTFYKSRYDGTTAEALAPNGMANYKLRQSDSFFSQ
                     REATANLPHAADAGSLVLDAQKALRLTSGINGQVRTGELGARVDILGQDIHLVDKTRA
                     TDTGLSLLAEDLSKLQVGSTVIGGERDDDKPGEIRVSAKTVTVEENAKVTMPAVVLVA
                     EKQVRVKTGAEVTATAAAANGQGAAITLDKPAALVRLAGEQALVNTASGADGSKLSLD
                     QGAVLKGNALLASAGETQLNSTLALTKGGALSMSGANIQLGGLNPDAPASNVLPTLNS
                     ATENKTLAASAGSWLYFSDDFFTNLGLGRVSLTAREQMRLAEGFNLSTGSLSLKAQSL
                     GRAAGKAGTAVTLKADNLSLSGASLAASPEPVVAGAGALNLEAKQISLGLNADPKTKA
                     PGESHLAINGFKQVNITGLNGISGQGKTQLEAAGDLTLTGSLSTQQQGDLSIKAQGTL
                     TTAQGSSATAAAGGLGGRISLEAAQLNIASRLQAASGQVQLTSEGALSLKAGASLDVA
                     GVSRRFGERTLYTPGGSLSLNSHSGSIELAEGAQLLFGAQAGKDA"
BASE COUNT          593 a          774 c          753 g          478 t
ORIGIN      
        1 attatttggc gagcaggttg agcttgcagg tgatcttcaa ttaaatggcg cagcgcaagc
       61 taattttgta agtagaggcg gacttttagc tcacggcgct tttggcggaa aagccagtag
      121 cggagctaac agagagaatc ctctcacttt gagcagcaat ggcgatttaa gcctaatcag
      181 tagcgtgctc tatcctggaa gcttttcaaa cataacaatt gaaagcacgg ccccaagcgg
      241 tacagtggaa attgccagca atggccagcc ggtacccaag gtttttagtg ccggtggcgc
      301 gctcaacatt aaatctaaac agatcgaaat cagcggcgcc ctaagcgccc cttttggcca
      361 ggtaaatctc accggtgatg atgtacacct cgccaaaagc gccctagtga atgtctccgg
      421 cgataaccaa ctgattccct tcggctccat tatcggtaac gacctagatt ggctttactc
      481 gctaagcccg ttggcgaaca caaccctgat cgacaacacc ccccttaaac aggtcaacat
      541 caaagccgaa cgcatcctgc tggatgaagg tagccgtatt aacctcgcag gcggcggcga
      601 cctattgggc tatcaattcg cccccggccc caccggcagt aaagacgtgc tggcgatcag
      661 tagcccaacc gaaggttttg cgcttttgcc tgcgggcaat tttgtcgcct atgacagcgc
      721 cctgcagggg caagatggca tagccaccgg cacccgcctg acgctcagca acaacccgct
      781 cttgcccgcc ggggactatt acctgctgcc ctcccgctat gcacttttgc ccggcgccta
      841 ctgggttaaa cccaccggca agctgcaggc ccccggcagc agtttcaccc agcctgatgg
      901 cggcatcacc cttaccggcc agctgggcaa ggcccacacc ccaatacaca gtgccgattg
      961 gcagggtttt accttttaca aaagccgcta cgacggcacc acagccgagg ccctggcccc
     1021 caacggcatg gccaattaca agctgcgcca aagcgacagc ttttttagcc agcgggaggc
     1081 caccgccaat ttgccccacg cggcagatgc gggcagccta gtgttggatg cccaaaaagc
     1141 tctgcgcctc acctccggca ttaacggcca ggtgcgtaca ggtgagctgg gggcgcgggt
     1201 ggatattctg gggcaggaca ttcacttagt agacaaaacc cgcgccaccg atacaggcct
     1261 tagcctattg gcggaggatc tctccaaact gcaggtgggc tccactgtga ttggcggtga
     1321 gcgggacgac gacaaacccg gtgagattag ggtgagtgcg aaaacggtca ccgtggagga
     1381 gaacgccaaa gtgactatgc ccgccgtggt attggtggcc gagaaacaag tgcgggtaaa
     1441 aaccggtgcg gaagttaccg ctactgctgc agcggccaat ggccaagggg cagctattac
     1501 cctggataag cccgccgcgc tggtgcgcct ggcgggggag caggccctgg ttaacaccgc
     1561 aagcggtgcc gatggcagca aactcagttt ggatcagggg gcggtattaa aaggcaatgc
     1621 cctgctggcc agcgccgggg aaactcaact taacagcacc ctagccctaa ccaagggcgg
     1681 ggctttaagt atgagtgggg ccaatattca gctggggggc cttaaccccg atgcgcccgc
     1741 ctccaatgtt ttgcctacgc tgaattctgc aaccgagaat aaaaccctgg cggcaagtgc
     1801 aggcagctgg ctgtatttct ccgatgactt cttcaccaac ctgggccttg gccgtgtttc
     1861 cttaactgcc cgcgagcaga tgcgcctggc ggagggcttt aaccttagca ccggtagcct
     1921 gagcctcaag gcgcaaagcc ttgggcgggc ggcaggcaag gcgggaacag ctgtgaccct
     1981 aaaggccgat aacctcagcc tcagcggtgc gagccttgcc gcaagcccag agccggtagt
     2041 agctggtgct ggggccctca atctggaagc caaacaaatc agcctcggcc tgaatgcgga
     2101 cccaaaaacc aaagcccccg gtgagagcca cctggctatt aacggcttta aacaggtgaa
     2161 catcacaggg cttaatggca tcagcggcca gggtaaaacc cagttggagg cggcagggga
     2221 cttgaccctt accggcagtt tatccaccca acagcagggc gatttaagca tcaaggccca
     2281 gggcactctc accacggctc agggcagcag tgcaactgcc gcagccgggg gcctcggcgg
     2341 gcgcatcagc ctggaagccg cgcaactgaa tatcgccagc aggctgcagg cggcctccgg
     2401 ccaggtgcag ctgaccagtg agggcgctct gagccttaag gccggggcga gcctggatgt
     2461 ggcaggcgta agccgccgct ttggcgagcg cactctttat acccccggcg gcagcttgag
     2521 ccttaacagc cacagcggct ccattgaatt agccgagggt gcccagctgt tatttggcgc
     2581 ccaggcgggt aaagacgc
//
LOCUS       DAOQGS010000594         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_25061_length_2597_cov_5.975610, whole genome shotgun sequence.
ACCESSION   DAOQGS010000594 DAOQGS010000000
VERSION     DAOQGS010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25061_length_2597_cov_5.975610"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="PKZ52_16645"
     CDS             <1..1922
                     /locus_tag="PKZ52_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG61454.1"
                     /translation="EELLSGDDTNTLEGVQERLNTALDRVGLLSKTGIADTSQIKKNL
                     KEFVAFATTRYQNQKSHNDKAEELESIFDDAYASYIVNAKQAQALTEQGITNNINSLK
                     AQTRAAFWVNCASLVFGVGLVMLAVFLGRRTIAAPLMQVEQQVSQLASGLRDLNSPID
                     GQARRDEIGGVAKAADRFRKSIIDKQIADAKAAAERRQIEAKLAQEEALRAQETRQRE
                     EESRQQAQAQREAEAAARSRAEREENARKEQARNEEEARLRAEQKAEAQRQAAAAAER
                     QAALEREETARLAAEEALASSVSQFATAIAEGRLSERVARSGGTGARARMEQSLNTMA
                     STLETVLNDLAKGTNALAQGNLSQTLTTPFLGVFEQLRQDFNNSCKNLSALVTNIHHS
                     AEAVASGSGEISVSNENLSQRIEEQASSVEQIARTVDELVDQIKQATENAEHTSQVAI
                     AANQKASDGNVILQNTIKAMGQIEMSSRKIGEIIGVIDEIAFQTNLLALNAAVEAARA
                     GEQGRGFAVVAGEVRNLAQRSADSARQIKSLIGDSLEKVSNGSVLVTQTAKSIEELSG
                     LIQSSQNAMGKISHSARQQYMGVEEIRRSIAQIETITQQNAALVEEATATSEALADES
                     RELRAMFNQFTLASA"
     gene            2056..>2597
                     /locus_tag="PKZ52_16650"
     CDS             2056..>2597
                     /locus_tag="PKZ52_16650"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNG61455.1"
                     /translation="MRWDNERRSDNVEDQRFGSSQPRGALGAAPLLLRLLPFLLQTRF
                     GRSLLIIGALVYFGGKMLGIDIAPLLLGGTVAPHQTEQAAPPAQDPLADFVSVILAST
                     EDTWQSIFHNNGRQYQRPKLVLFRQQVRSACGFAEAAMGPFYCPGDQKLYIDLSFYQV
                     MKTRLGAPGDFAQAYVIAHEV"
BASE COUNT          674 a          745 c          680 g          498 t
ORIGIN      
        1 tagaagaact gctcagcggc gatgacacta acactttaga aggcgtgcaa gagcgcctga
       61 ataccgccct tgaccgggtt ggcctgttga gtaaaacagg catagccgac accagccaaa
      121 ttaagaaaaa tctcaaagaa tttgtcgcgt ttgccaccac ccgctaccaa aatcaaaaaa
      181 gccacaatga taaagccgaa gagttggaaa gtatttttga cgacgcctac gccagctata
      241 tagtcaacgc caagcaggcg caagcgctta ccgaacaggg catcaccaac aatattaatt
      301 ccctcaaagc gcagacccgc gcagcctttt gggtcaattg cgcaagcctt gtttttggcg
      361 tcggcctcgt gatgctggcg gtatttttag gccgcagaac catcgccgcg cctttgatgc
      421 aggttgaaca acaggtgagc caattggcct ccggcctgcg ggatttaaat tccccaatcg
      481 acggccaagc ccgccgcgat gaaattggcg gcgttgccaa ggccgccgac cgctttcgca
      541 aatccatcat cgataaacaa attgccgatg ccaaagctgc cgcagagcgc cgccaaatcg
      601 aggcgaaatt agctcaggaa gaggccctgc gggcgcagga aacccgtcag cgggaagaag
      661 agagccgcca gcaggcccag gcccagcgcg aagcggaagc cgccgcccgc agccgcgccg
      721 agcgggagga aaatgctcgc aaagaacagg cgcgcaacga ggaagaagcg cgcctgcgcg
      781 ccgagcaaaa agccgaggcc caacgccagg cggcggcagc ggccgagcgc caggccgccc
      841 tggagcgcga ggaaaccgcc cgcctcgccg ccgaagaggc cctcgccagc agcgtcagtc
      901 aatttgccac cgccatagcc gaaggccgcc tatcagagcg cgttgcgcgc tccggcggca
      961 ctggcgcccg cgcccgcatg gaacagagcc ttaacaccat ggcgtccaca ttggagacgg
     1021 tattaaacga tttggccaaa ggcactaacg ccctcgccca aggcaattta tcgcaaacgc
     1081 ttactacgcc ttttttaggc gtgtttgaac aattgcggca ggattttaac aacagctgta
     1141 aaaatctttc cgccctggtt accaatattc atcacagtgc cgaggcagtg gcatccggca
     1201 gcggagaaat tagtgtgagc aatgaaaact taagccagcg aattgaagaa caggcatctt
     1261 ccgtagagca aattgcccgc accgtggatg agcttgtcga tcaaattaaa caggccactg
     1321 aaaacgctga acacaccagc caagtagcca ttgccgccaa tcaaaaagcc agcgacggca
     1381 atgttatttt gcaaaatacc attaaagcca tggggcaaat tgaaatgtcc tcccgcaaaa
     1441 ttggcgagat tatcggcgtc atcgacgaaa tcgcctttca aactaattta ttagccttaa
     1501 atgcagcggt ggaagccgcc cgcgccggtg agcaggggcg cgggtttgcc gtggtggcag
     1561 gcgaggtgcg caatttagcc cagcgctcgg cggattcggc gcggcaaata aaatccctca
     1621 ttggcgatag cttagaaaaa gtcagcaacg gctcagtgtt ggttacccaa accgctaaat
     1681 ccatcgaaga attaagcggt ttaattcaat cctcacaaaa tgctatgggc aaaatctccc
     1741 acagcgcccg ccagcaatat atgggtgtgg aagaaattcg ccgcagcatt gcgcaaattg
     1801 aaaccatcac ccagcaaaat gccgctctag tggaagaggc caccgccacc agcgaagccc
     1861 tggccgatga gtcccgcgaa ttgcgcgcca tgtttaatca atttaccttg gcaagcgctt
     1921 aaacgcggcg cattgagcac acaatattgt tctaagggcg aattaacagc gcgttaattc
     1981 gcccgcccca gcgcccccgc taccgcaccc aactggagta cactggcacg cgaaattaac
     2041 agccttaggt gacctatgcg gtgggataat gaacggcgca gcgacaatgt tgaagatcaa
     2101 cgctttggat catcgcaacc gcgcggcgcc ctaggtgccg ccccacttct gctgcgcctg
     2161 ctgccttttt tattgcaaac ccgttttggt cgcagcctgc taattatcgg tgcactggtg
     2221 tatttcggcg gcaaaatgct cggcattgat atagcacctt tattgctcgg cggcactgtg
     2281 gcgccacacc aaaccgaaca agcagcccct ccagcgcagg atcctctggc cgattttgtc
     2341 tcggttattt tggccagcac cgaggacacc tggcagagca tttttcacaa caacggacgg
     2401 caatatcagc ggcccaaatt agttttattt cgccaacagg tgcgctcagc ctgcggtttt
     2461 gccgaagcgg ccatggggcc gttttattgt ccaggcgatc aaaaactcta tatcgattta
     2521 agtttttacc aggtgatgaa aacccgcttg ggtgcaccgg gagattttgc gcaagcctat
     2581 gtcattgccc acgaggt
//
LOCUS       DAOQGS010000595         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_25096_length_2595_cov_5.441732, whole genome shotgun sequence.
ACCESSION   DAOQGS010000595 DAOQGS010000000
VERSION     DAOQGS010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25096_length_2595_cov_5.441732"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..624
                     /locus_tag="PKZ52_16655"
     CDS             136..624
                     /locus_tag="PKZ52_16655"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNG61456.1"
                     /translation="MVRHFLLFSSLSLLINCGAAPTSTTSGTSKSALAEHIALLQKNK
                     ALWKEANIRDYEFTYQATGGCGDPMPPVVISVKDGVVSSVYVPDFGTYESIGSWPSID
                     GVFDSLTQAAVKNPLVFALGASTPNTPPAFDAKYGFPTKISVDPSDAKCDDSQFTIRD
                     FK"
     gene            752..1423
                     /locus_tag="PKZ52_16660"
     CDS             752..1423
                     /locus_tag="PKZ52_16660"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNG61457.1"
                     /translation="MFTGIIQAIGTVAALQPKGGDLSLYIRAPNSASWDWSHVALGDS
                     IASNGVCLTAVNLPGDGFWADASNETLTLTSLKHWRIGQKINLELALTPQSRLGGHIV
                     SGHVDGLGQVIKREADARSERFTLRAPDELAKYIAHKGSVTVDGVSLTVNAVNGAEFE
                     LNIVPHTLSHTVMGTYQPGHWVNLEVDLIARYLERLLLGERAAHSDGASNLTAAFLAQ
                     NGYWK"
     gene            1429..2544
                     /gene="ribBA"
                     /locus_tag="PKZ52_16665"
     CDS             1429..2544
                     /gene="ribBA"
                     /locus_tag="PKZ52_16665"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNG61458.1"
                     /translation="MQLNTTAELIEDIRQGKMIILMDDEDRENEGDLIMAAEMVRPED
                     INFMAMHARGLICLPMTPQRCQLLELPLMVKENQAAHATNFTVSIEAAQGVTTGISAS
                     DRARTIQAAVSRHAKPKDIVQPGHIFPLMAQPGGVLSRAGHTEAGCDLARLAGFEPAA
                     VIVEIMNEDGSMARRPDLEKFAQSHNLKIGTIADLIHYRTLNERTVICTNSREVDTLH
                     GSFTLKTYKDTARNECHFALVKGAINPEADTLVRVHVGSTLRDVLGIQQPGAPGPAWT
                     CQSALARVAKEGAGVVVLICHNETTDDVEESIDWLLTGKVRRPNVELAYKQVGTGSQI
                     LKDLGVCKMRLMSAPYKFNGISGFNLEVTGYVEHPGE"
BASE COUNT          610 a          720 c          692 g          573 t
ORIGIN      
        1 gggaggccat tttgccatct ggcgcggggg ttttacacaa ttctacgaag agccatattt
       61 tatgctgggt tcggctgaac atttaattat tcaagttatt gatccttaca aatctttaaa
      121 aaatggagaa tttatatggt gcgtcacttt cttttatttt catctttaag tttgcttatt
      181 aactgcggcg ctgcgcctac ttcaacaaca tcgggcacga gtaaatcagc gctcgctgaa
      241 cacatagcct tactgcaaaa aaataaagct ctgtggaaag aggccaatat tcgcgactat
      301 gaattcactt atcaggcgac aggcggttgt ggcgatccta tgcctccggt tgttatcagc
      361 gtgaaagatg gcgtggtgtc gtctgtttat gttcccgatt ttggcaccta cgagtcgatc
      421 gggtcttggc catctataga cggagtgttt gacagcctaa ctcaagctgc ggttaagaac
      481 ccgttggtat tcgccctggg ggcctcaacg cccaacacgc ctcctgcatt cgatgcaaaa
      541 tatggctttc ccaccaaaat atcggttgat ccttcagacg caaagtgtga cgacagccaa
      601 ttcactatca gagattttaa atagcaggta gcacctctca actttggcgt aaaatagtcg
      661 cccggcttgc tgtgtctgat tgtgagtcgg ttatccctgt aattcgcggg atttcggcca
      721 agcaataccg ccatcaggtt gagaggtttt tatgttcacc ggcattatcc aagccatcgg
      781 cactgtagcc gccctgcaac ctaagggcgg cgatttaagc ctgtatattc gcgccccaaa
      841 cagcgccagc tgggactgga gccatgtggc cctaggggat tccatcgcca gtaatggcgt
      901 ttgcctcacc gctgtgaact tgcctggcga tggcttttgg gcggatgcct ccaatgaaac
      961 cctcacgctc accagcctta aacactggcg catcggccaa aaaattaatt tggaactggc
     1021 cctcacccct caatcccgct taggcggcca tatagtctcc ggccatgtgg atggccttgg
     1081 ccaggtgatt aagcgtgaag ccgacgcccg ctccgagcgc tttaccctgc gggcaccgga
     1141 tgagctggcc aaatacatag cccacaaagg ttcggtcaca gtggacggcg taagccttac
     1201 ggttaacgcg gtgaacggcg ctgagtttga gctgaacata gtgccccaca ccctaagcca
     1261 cacggtgatg ggcacctatc aacccggcca ctgggtaaat ttagaggtgg accttatcgc
     1321 ccgctacctt gagcgcctac tcttagggga aagggccgcg cacagcgacg gcgcgtccaa
     1381 tttaacagcg gcatttttag cgcaaaacgg ttactggaaa taaattctat gcaattaaat
     1441 accaccgctg agctgatcga agatattcgc cagggcaaga tgattatcct catggacgat
     1501 gaagaccggg agaacgaagg cgatctgatt atggccgccg agatggtgcg ccccgaggac
     1561 atcaacttta tggccatgca cgcccgcggc ctgatttgtt tgcccatgac gccccagcgc
     1621 tgtcagctgc tggagctgcc tttgatggtg aaggaaaacc aagccgccca cgccactaac
     1681 tttaccgtct ccattgaagc ggcccagggg gttaccaccg gcattagcgc tagcgatcgc
     1741 gcccgcacca ttcaagccgc cgtgagccgc catgccaagc ctaaagacat agtgcagccc
     1801 gggcatatct tcccgcttat ggcccagccg ggcggggtgt taagccgcgc tggccacaca
     1861 gaagccggtt gtgatttagc gaggctggcc gggtttgaac cggcggcggt gattgtggaa
     1921 attatgaatg aagatggcag catggcccgt aggccggacc tggagaaatt tgcccaaagc
     1981 cataatttaa aaatcggcac catcgccgac ctgattcact accgcaccct caatgagcgc
     2041 acggtgatct gcaccaacag ccgggaagtg gacaccctcc acggcagttt caccctaaaa
     2101 acttataagg acacagctcg caacgagtgc catttcgccc tcgtcaaagg cgccatcaac
     2161 cccgaggcgg acaccctggt gcgggtgcat gtgggttcca ccctgcggga tgtgttgggc
     2221 attcaacagc ccggcgcccc aggcccggcc tggacctgtc aatccgccct ggcccgcgtg
     2281 gccaaagagg gggctggggt ggtggtgctc atttgccaca acgaaaccac cgacgatgtg
     2341 gaagagagca tcgactggct gctcaccggc aaggtgcgca ggcccaatgt ggagctggcc
     2401 tacaaacagg tgggcacagg ttcccagata ctaaaagacc tgggggtgtg taaaatgcgc
     2461 ctgatgagcg ccccctataa atttaacggc atttccggtt ttaacctcga agtgaccggc
     2521 tatgtggagc acccaggcga gtaggtcaat gagatggact ccccctctct aacaacccgg
     2581 catcgaagtg ccaca
//
LOCUS       DAOQGS010000596         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_25674_length_2559_cov_4.432907, whole genome shotgun sequence.
ACCESSION   DAOQGS010000596 DAOQGS010000000
VERSION     DAOQGS010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25674_length_2559_cov_4.432907"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKZ52_16670"
     CDS             <1..319
                     /locus_tag="PKZ52_16670"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG61459.1"
                     /translation="GHLKLELPQAVGSDDAPKVNRIEVIVDPKGGYSLNGQALINSQA
                     DTLLRAIEKLAISEKKPPFVVTADANVPYQAVVTVMEAAGKSGFANLQLTTQIPGGPA
                     KTP"
     gene            319..1311
                     /gene="lpxK"
                     /locus_tag="PKZ52_16675"
     CDS             319..1311
                     /gene="lpxK"
                     /locus_tag="PKZ52_16675"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNG61460.1"
                     /translation="MEQAVVKQWYTKPTWLWVFLPLMPLLWLLRLLRRRLLTALQPRL
                     PVPVVIIGNITVGGTGKTPVLIALAQALMALGLKVGVVSRGYGGSFSGAFLAVDNSCD
                     PDLCGDEPALLAQALSCPVCIGRDRVAAALELVRRGCQVILSDDGLQHYRLKRDIEII
                     LLDKSRGLGNGWCLPMGPLREPARRLQEADWVLINGGAGPDGFVLQPRRWRQLATNEA
                     FPFQPWPWGEQTQVCALAGIGNPERFFASLEGWGLQVTGRALPDHHRFNPMDFAFADG
                     QPLLITAKDAVKCRMLAPPNTWVLDVDAQLPAAFLRDFCARVQALCPPEQEHTP"
     gene            1308..2093
                     /gene="kdsB"
                     /locus_tag="PKZ52_16680"
     CDS             1308..2093
                     /gene="kdsB"
                     /locus_tag="PKZ52_16680"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNG61461.1"
                     /translation="MTYTVIIPARFGSSRLPGKPLKDLGGKSMVQRVYECARQSAASR
                     VIVATDDQRIADVVAGFGGEVCMTGSHHESGTDRLHEVVTRLDLPAEHIVVNVQGDEP
                     LIPAAVIDQVADNLACRPAVSAATLCEKIHRLEDCFDPNIVKVVCDHAGLALYFSRAP
                     IPWHRDGYSATPQLMPAGINPLRHIGIYAYRVSLLQEFVRWPLAPLEACEKLEQLRIL
                     ANGEAIHVAEAVEAVPGGVDTELDLKRVRQYLAQQEAGGKGAL"
     gene            2090..>2559
                     /locus_tag="PKZ52_16685"
     CDS             2090..>2559
                     /locus_tag="PKZ52_16685"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNG61462.1"
                     /translation="MSTKILFVCLGNICRSPTAHGVMEAKIQQQRLNSRIEVDSAGTS
                     DWHIGKRPDSRTMACAKRRGYDLSPQRARQVCREDFAVFDYILAMDNNNLRELQQLKP
                     AGFSGHLGLFLEVLTEQPLREMPDPYYGDTAAFEQVLRLSEAACDAWLTRMLAA"
BASE COUNT          497 a          797 c          781 g          484 t
ORIGIN      
        1 gggccattta aagttggaac tgccccaggc ggtgggcagt gatgatgcgc ccaaggtgaa
       61 tcgcatcgag gtgattgtgg acccaaaagg cggttattca ttaaacggcc aggcgctgat
      121 caacagccaa gctgacacgc ttttgcgggc cattgaaaaa ctcgccatca gcgaaaaaaa
      181 accccccttt gtggtgaccg ccgatgccaa tgtgccctac caagcggtgg tgacggtgat
      241 ggaagcggcg ggcaaatcgg gctttgccaa tttgcagctc accacacaaa tccccggcgg
      301 cccggctaaa accccctgat ggagcaagcg gtagtcaaac agtggtacac aaaacccacc
      361 tggctgtggg tttttttgcc tttaatgccc ctgctgtggc tgttgcgcct gctgcgccgc
      421 aggttgttaa ccgcgctgca accgcgcctg ccggtgccgg tggtgattat cggcaatatc
      481 acggtgggcg gcaccggtaa aacccccgta ctcatcgccc tcgcccaggc gcttatggca
      541 ctgggtttaa aggtgggtgt ggtgtctcgc ggctacggcg gcagcttcag cggggcattt
      601 ttagcggtgg ataacagctg cgaccccgat ctctgtggcg atgaaccggc gctgttggcg
      661 caggccctca gctgccctgt ctgcataggc cgcgaccgcg tggccgccgc cttggagctg
      721 gtgcgccgcg gctgccaggt gatcctcagc gacgacggcc tgcagcacta ccgcttaaag
      781 cgggatatcg aaatcattct gttggataaa agccggggcc tgggcaatgg ctggtgcctg
      841 cccatggggc cattgcggga gcccgcccgg cgcttacagg aggccgactg ggtactgatc
      901 aacggcggcg cggggccgga cggctttgtg ctgcagccgc gccgctggcg ccagttggcc
      961 actaatgaag ccttcccatt ccaaccctgg ccctggggag agcaaactca agtatgcgcc
     1021 ctggccggta tcggcaaccc cgagcggttt tttgccagtt tagaaggctg gggccttcag
     1081 gtgacgggcc gcgctctgcc cgatcaccac cgctttaacc ccatggactt cgcctttgcc
     1141 gatggccagc ccctattaat caccgctaag gatgcggtta aatgccgcat gctcgccccg
     1201 ccgaatacct gggtgctcga tgtagacgcc cagctgcccg ccgcgttttt gcgcgatttt
     1261 tgcgcccgcg tgcaggcgct ttgcccaccc gaacaggagc acaccccgtg acctataccg
     1321 tcattatccc cgcccgcttc ggctccagcc gcctgcccgg caaacccctc aaagacctag
     1381 gcggcaaaag catggtgcag cgagtgtacg aatgcgcccg ccaaagcgcc gccagccgtg
     1441 tgattgtggc caccgacgac cagcgtattg ccgatgtggt ggctggcttc ggcggcgaag
     1501 tctgtatgac cggcagccac catgagtccg gcaccgaccg gctgcacgag gtggtgacgc
     1561 gcctcgattt accggcggag cacatagtgg tgaacgtgca gggggatgaa cccctgattc
     1621 ccgcagcggt gatcgaccag gtggcggaca atctcgcctg ccgcccggcg gtgagcgccg
     1681 ctaccctatg cgagaaaatc caccgcctgg aagactgctt cgacccgaat atagtgaagg
     1741 tggtgtgcga ccacgcaggc ctcgccctgt atttctcccg cgctcccatc ccctggcacc
     1801 gggacggcta cagcgccacg ccccagctga tgccagcggg aattaaccct ctgcgccata
     1861 taggcattta tgcctaccgg gtaagcctgt tgcaggagtt tgtccgctgg cccctggcgc
     1921 ccttagaagc ctgcgaaaaa ctcgaacaac tgcgcatcct cgccaacggt gaagccatcc
     1981 atgtggccga agcggtggaa gccgtgcccg gcggggtgga tacggagctg gacttaaagc
     2041 gcgtgcgcca gtacctggcc cagcaggaag caggcggcaa gggggcgcta tgagtaccaa
     2101 aatactcttc gtgtgcctgg gcaatatctg ccgcagcccc accgcccacg gcgtgatgga
     2161 ggcaaaaatc cagcagcagc ggcttaactc gcgtatcgaa gtggattcgg cgggcacctc
     2221 tgactggcat ataggcaaac ggcccgatag ccgcaccatg gcctgcgcca agcgtcgcgg
     2281 ctatgacctc agcccccagc gggcgcgcca ggtgtgtaga gaagacttcg ccgtgtttga
     2341 ttacatcctg gctatggata acaacaatct gcgggaatta cagcagttaa aaccggcggg
     2401 gttcagcggc catttaggtt tgtttttgga ggtattaacc gagcagcccc tgcgggaaat
     2461 gccagacccc tactacggcg ataccgccgc ttttgagcag gtattgcgcc ttagcgaagc
     2521 cgcctgcgac gcttggctta cgcgcatgtt agcggccca
//
LOCUS       DAOQGS010000597         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_25981_length_2542_cov_4.057097, whole genome shotgun sequence.
ACCESSION   DAOQGS010000597 DAOQGS010000000
VERSION     DAOQGS010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25981_length_2542_cov_4.057097"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="prmC"
                     /locus_tag="PKZ52_16690"
     CDS             <1..805
                     /gene="prmC"
                     /locus_tag="PKZ52_16690"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNG61463.1"
                     /translation="AATLAALWCLAQGKSVSAIAACQTSLLPLSFEEEIRLQQLISQR
                     LSGVPLAHITGRQHFAGLELLASPDALIPRAETEILLRLALSCLEPKNGQVLQLLDVC
                     TGSGNVALALAHSLPQAFVRGADLSGEAVGLAQQNAQHLNLQARLEFKAGDLCGPFSS
                     AEFSEYFDLISCNPPYIMANNVKKMPKEIADFEPSLAFDGGPMGIAIIERIISEAHPL
                     LKPGGFLCLEVGAGQGPFIEKRLKKSALYTDVITACDGHNVVRALAARK"
     gene            852..1835
                     /gene="nadE"
                     /locus_tag="PKZ52_16695"
     CDS             852..1835
                     /gene="nadE"
                     /locus_tag="PKZ52_16695"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNG61464.1"
                     /translation="MQTQSLARALDFDPAAEVARISASIRELLRSKINRRGLVVGISG
                     GVDSAVSAALCVKAIGANKVFGLLMPEQDSAPGTTPLGEVVARHLGINYEIKNISSTL
                     EAIGCYQWRDAAIKKLFPDYDGTWKNKIVIDGGAQGKINHFKLIVQAPDGSLQSAILP
                     LNEYLTIVAATNFKQRIRKTTEYFHAERLNYAVIGTPNRLEYDQGFFVKGGDGLADIK
                     PIAHLYKTQVYALARFLELPEAICNSIPSTDTYSLQQGQDEFYFALPYHQMDIALWAY
                     NHQVPAEDVATELNLSLEQVHYVFKDIESKRKTTAYLHLAAQLVEPVDLRG"
     gene            complement(2030..2248)
                     /locus_tag="PKZ52_16700"
     CDS             complement(2030..2248)
                     /locus_tag="PKZ52_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61465.1"
                     /translation="MLLPLLKGEAGWGFWAGASKEVLTLNLVTNKTICPLASVSTSKN
                     PKANQQVRDLIDGQFAQTSTCPTSITKL"
BASE COUNT          627 a          612 c          619 g          684 t
ORIGIN      
        1 agcagctact ttggcagccc tttggtgttt ggctcagggc aaatctgttt ccgccatagc
       61 ggcttgccaa accagtctgc tgcctttgtc cttcgaagaa gaaatccgct tacagcaatt
      121 aatttctcag cgcttaagcg gcgtgcccct cgcccatatc accggccgcc aacattttgc
      181 aggcttggag ttacttgcta gccccgatgc gcttattccc cgcgcagaaa cagaaatttt
      241 gctgcgctta gccttaagtt gtttggagcc taaaaatggt caagtgctac agctgttaga
      301 tgtgtgcaca ggctctggca atgtggcttt ggctcttgcc cactccttac cccaagcgtt
      361 tgtgcgcggg gcggatttat ctggtgaggc ggtggggctt gctcagcaga atgcgcagca
      421 ccttaattta caggcgcggc tggaatttaa agcgggcgat ttatgcggcc cttttagcag
      481 tgctgaattt agcgaatact ttgatttaat cagctgcaac ccgccgtata ttatggctaa
      541 taatgtaaaa aaaatgccca aggaaatcgc cgattttgag ccgtctttag cctttgacgg
      601 cggccctatg ggcatagcca ttattgagcg catcatcagc gaagcccatc ctttgttaaa
      661 acctggcggt tttttgtgtt tagaagtggg tgcagggcag gggccgttta ttgaaaaacg
      721 cctgaaaaaa tccgccctgt acacagatgt gatcactgcc tgtgatggcc ataatgttgt
      781 gcgcgccctg gcggcgcgca aataatgaat aaaactcaat aaataaaact atcgatgtaa
      841 atggagcaaa catgcaaacc caatccttag cccgcgccct cgattttgac cctgcggctg
      901 aagtggctcg cattagcgca agcatccgcg aattattgcg cagtaaaata aaccgccgcg
      961 gtttagttgt cggtatttcc ggtggagtcg atagcgctgt aagtgccgcc ttatgtgtga
     1021 aggccattgg cgccaataag gtatttggcc ttttgatgcc ggagcaggat tctgcccctg
     1081 gcaccacgcc cctaggtgag gtggtggccc ggcatttggg cattaattac gaaattaaaa
     1141 atatctcgtc tactcttgag gccatcggct gttatcagtg gcgcgatgcc gctataaaaa
     1201 aattattccc tgactatgac ggcacctgga aaaataaaat cgttatcgac ggcggcgccc
     1261 agggcaaaat taatcacttt aaattaattg tgcaggcgcc ggatggctca ttgcagtcgg
     1321 ctattttacc gctcaatgaa tacctcacca ttgtggccgc caccaatttc aaacagcgca
     1381 tccgcaaaac taccgagtat ttccatgccg agcgattaaa ttacgctgtg attggtaccc
     1441 ccaaccgttt agagtacgac caagggtttt ttgtgaaagg tggcgatggc ttagccgaca
     1501 ttaaacccat tgctcacctg tataaaactc aagtttatgc gctcgctcga ttcttagaat
     1561 tacccgaggc catctgtaat tcgatcccgt caaccgatac ctacagtttg caacagggcc
     1621 aggacgaatt ttatttcgcg ctgccctatc accaaatgga tatcgccctc tgggcctata
     1681 atcaccaggt gcctgcggaa gacgtggcaa cggaattaaa tctgagcttg gagcaagtgc
     1741 actatgtttt taaagacatt gaaagcaaac gcaaaactac tgcttatttg catcttgcgg
     1801 ctcaattagt ggagccggtg gatttgcggg gataagtgac tgcgagctat tggcgtgttg
     1861 cgcctgtgga tttagcgcgt tgcgactgtg taattggcgc gttgcgtttg tgtaattatt
     1921 taactggggg cagggcttcc tgcccttgag gcgtttttta tgctgggcta gcagtataaa
     1981 aaacgcaatc taaaagatta tttatttgca acatctgcaa aagagcgcgt tacaacttgg
     2041 taatgcttgt tgggcaggtg ctggtttgtg caaactgccc atcaattaaa tctcgcacct
     2101 gctgattggc cttggggttt ttactggtgg agacgcttgc taagggacaa attgttttgt
     2161 tagtcaccag gtttagggtt aatacttctt tgctagcgcc tgcccaaaac ccccacccag
     2221 cctccccctt caaaaggggg aggagcaaaa aaccttaaca ctaaggcgct ttgctattgc
     2281 tccagatgat tttgctccac cccttattaa ggggaggttt tatgtataag cgcaaaatca
     2341 gctaaaaaat gttacaaaac gattaactta atgccattac ctttgcacag agagacaaag
     2401 gcaggacgct agggaacctc tgaaaaatta ccttactcgc cctggcggtg ttgaatttgg
     2461 cagaaaaatg ctcgtttact gcgtctaaaa ctcggcaatt gctcctgcat tgctctacct
     2521 cctgcctccc tgcagtcgtc cg
//
LOCUS       DAOQGS010000598         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26202_length_2529_cov_8.305982, whole genome shotgun sequence.
ACCESSION   DAOQGS010000598 DAOQGS010000000
VERSION     DAOQGS010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26202_length_2529_cov_8.305982"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKZ52_16705"
     CDS             <1..333
                     /locus_tag="PKZ52_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61466.1"
                     /translation="RDQCPALVVFPETEPITERSNNRVRRELHLRLVALTRTLPPLSS
                     EAEADRLLTAAHTALMADVNFGGLALSVHEVETEWDIEDADASACAMTTRYQIIYRTL
                     DNNLTLSG"
     gene            338..535
                     /locus_tag="PKZ52_16710"
     CDS             338..535
                     /locus_tag="PKZ52_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61467.1"
                     /translation="MHIQLLKPHTHDGQMLAPGDSLHLAVDLAQWLINLEVACACPSE
                     VSVPAASPSTSIKRFTEEVSL"
     gene            532..1287
                     /locus_tag="PKZ52_16715"
     CDS             532..1287
                     /locus_tag="PKZ52_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61468.1"
                     /translation="MSFYASFQGRVYLGKRDSNGRPIDIRSPGNVAELKLSLKTEVLA
                     HYENQSGNRSLDHRMIKTKAATVSLVIEEFTRDNLALALYGTAVTTANGTVTDEPVGG
                     STPVIGDRYCLAHPKISTLVVKDSAAVPATLVEGSDYTADTDFGALQWLDLTGFTPPL
                     KASYAFGQVDEIGIFTQPLPERFLRLEGINTAQNNAPVLVELYRVGFDPLKELGLITD
                     DYNKFELEGSLLADATKPYDTELGQFGRIVQIR"
     gene            1294..1758
                     /locus_tag="PKZ52_16720"
     CDS             1294..1758
                     /locus_tag="PKZ52_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61469.1"
                     /translation="MNATNPINSSVFATQNLFAALPPVPLQLTLAGETLEITPLRVGE
                     LPAFSRAITPISSAFSAEPDWVRLMSHNGDALIEALAIAARRPAQWVGGLALDEGIAL
                     AEAIFEANLDFFIHRVVPMIVGSSTRIVQTLSSLGPTVSSSSSAPDTSTPLS"
     gene            1992..>2529
                     /locus_tag="PKZ52_16725"
     CDS             1992..>2529
                     /locus_tag="PKZ52_16725"
                     /inference="COORDINATES: protein motif:HMM:NF040424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6441 family protein"
                     /protein_id="HNG61470.1"
                     /translation="MRISLAASGLLDPQRLNSWLPERRRAIRAAVENAMKTVGKDIVA
                     AGRKQMESVFNVRSAGFTRSLRHRLYTAKADQLPALKIGSPIPWLGIHVRGGTVAGRL
                     LIPLLPEHQRIGRKAFGRVVESLIRSGNGFFIQKNGKVILMAENIAENSRQLTRFKRA
                     ERQRTGQRAIKRGQEIPIA"
BASE COUNT          535 a          807 c          684 g          503 t
ORIGIN      
        1 cgcgaccagt gcccagccct ggtggtgttt cccgaaacgg agcccatcac ggagcgctcc
       61 aacaaccgcg tgcggcggga attacacctg cggctggtgg cgttgacccg caccctgccc
      121 ccgctcagca gcgaagccga ggcggatcgg ttattaacgg cggcccacac ggccctgatg
      181 gccgacgtta attttggtgg tctggcgctg agcgtgcacg aggtggagac cgaatgggat
      241 atcgaagacg ccgatgccag tgcctgcgcc atgaccacgc gctaccagat tatctaccga
      301 accctcgaca acaacctgac gctatccggt taatcccatg catatccaac tactcaaacc
      361 gcacacccat gacggtcaga tgctggcgcc tggcgattcg ttgcatctgg cggtggatct
      421 ggcccagtgg ctcatcaatc tcgaggttgc ctgcgcatgt ccatccgaag tcagcgtacc
      481 cgctgcatcg ccatccacat caatcaaacg ctttactgag gaggtatcct tgtgagtttt
      541 tatgcctcgt ttcaaggccg ggtgtatctc ggcaaacgcg attccaatgg tcgtcccatc
      601 gatattcgca gccccggtaa cgtggcggaa ttaaagctgt ccctcaaaac cgaggtgctc
      661 gcccattatg agaatcaatc cggtaaccgc tccctcgatc accgcatgat caaaaccaag
      721 gccgcaaccg tcagcctggt catcgaggaa tttacccgcg acaacctcgc gctggcactc
      781 tatggcaccg ccgtcaccac tgccaatggc accgtcaccg atgaacccgt gggtggctcc
      841 acaccggtca ttggcgaccg ctattgtttg gcacacccca aaatttcaac cctggtcgtt
      901 aaggattcgg ccgctgttcc agcgacgctc gtcgaaggca gcgattacac cgccgacacc
      961 gattttggtg cgcttcaatg gctggatctc accggtttta cgccgccctt aaaagccagt
     1021 tatgcctttg gtcaggtgga tgaaatcggc atttttacgc aacccttgcc cgagcgtttt
     1081 ttgcgtctgg agggcattaa caccgcgcaa aacaatgcgc cggtactggt ggagttgtat
     1141 cgggtggggt ttgatccgct taaagagctg ggactgatta ccgacgatta caacaagttt
     1201 gaactggaag gatcgctcct ggcggatgcg actaagccct atgacactga gctcggccaa
     1261 tttggccgta tcgtgcaaat tcggtaaata cctatgaatg cgacgaaccc gatcaactcc
     1321 agcgtattcg caacccagaa tttatttgcg gccctaccgc cggttcccct acagctgacc
     1381 ctggctggag aaacccttga gatcacgccc ttgcgggtgg gtgaactccc ggcgtttagc
     1441 cgcgccatca ccccgatcag cagtgccttc agcgccgagc cggattgggt gcgcctgatg
     1501 agccacaacg gcgatgccct gatcgaagcc ctcgccatcg cggcccgccg cccggctcag
     1561 tgggtcggcg gcctggccct ggatgagggc attgccctgg cagaagcaat tttcgaggcc
     1621 aacctggatt tttttatcca tcgcgtggtg ccgatgatcg tcggcagcag cacgcggatt
     1681 gtgcagacac tgagctcact tgggccgaca gtctccagca gctcatcagc gccggacacc
     1741 agtaccccac tgtcctgacc tatacactcg cccaggtgcg cgccttttta gccgccatcc
     1801 atcagcaccg gcacgatgcc ctcgccaccg aatgggcact cctgctcacg gccacacgcg
     1861 gcagtgccca ggatatccag accctcagtc gcgacctgcg gcccagggca acacccgctc
     1921 ggccttcgtt cgccgctaaa ccaacacgtc actccaaata actcttaggt aagtaaacct
     1981 taagtaacta tatgcgcata agcctggccg cctccggcct gttagatccc caacgcctga
     2041 acagctggtt gccggaacgg cgcagagcca tccgggcagc ggtcgagaac gcgatgaaaa
     2101 ccgtcggcaa ggacatagtc gcggccgggc gcaagcagat ggagtcggtg tttaacgtac
     2161 gctccgccgg gtttacccgc tcgttgcgcc atcggctcta taccgcaaaa gccgaccagt
     2221 tacccgcctt aaaaatcggc tcaccgattc cctggctggg tatccatgtg cgtggcggca
     2281 cggtagccgg ccgcctgttg atcccactgc tacccgaaca ccaacgcatc ggtcgcaagg
     2341 catttgggcg ggtggtggag agtttgatcc gcagcggtaa cggctttttt attcagaaaa
     2401 acggcaaggt gatcctgatg gccgagaaca tcgccgagaa cagccgccag ctcacccgat
     2461 tcaaacgtgc tgaacgccaa cgcaccggcc agcgggccat caagcgcggc caggagattc
     2521 ccatcgcgg
//
LOCUS       DAOQGS010000599         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26204_length_2529_cov_4.573161, whole genome shotgun sequence.
ACCESSION   DAOQGS010000599 DAOQGS010000000
VERSION     DAOQGS010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26204_length_2529_cov_4.573161"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="PKZ52_16730"
     CDS             complement(<1..1653)
                     /locus_tag="PKZ52_16730"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNG61471.1"
                     /translation="MHLLLVLGGLLACLVLPALLPLAEQQAGRVLFAWRGPLVPPSEI
                     ALVTLNQQAAEALGQPRRLFSRRVHAQLINQLTAAGATLIVFDVAFKDARNEEEDAAL
                     EAAIKAANRVVLFDYLKRYQVDGGSKHADDGKADIEEIIPPLARFKQVALASGPFVLP
                     KAAGLVDHFYPVMHMQGEFTLSQPLIALSLVRPADWQLFLDRAQQAAPNLNSALPIWQ
                     QIHALNSAEREAFLRSCTEALCLSLWHSLNHPDGVAINFYGPPGHLPQWPIDQVLTMS
                     ADEQRSAFSGKIVYVGLVENQQTEQQDAYPTVYSDADGLDLSGVEISATALGNLLHKQ
                     MLMRTAYWQQAVLLMVWLGVLWLLSHRAGRFVWWAGALWHGLYLVAVYWAFVQQNYRL
                     PLAAPLVLSLVFYSCDISLRLRANKLRYHNLLTDLSNYMPAQAAKALSEQISNFAQHN
                     EVVSGVCLLTDIQGYTRLAEELPPAQLHELMNRYYAELIAEVKASGGIIGNIVGDALL
                     ALWLDTHISADACLRASKTARAIQKRLAAQADLHLLPTSCALH"
     gene            1771..2409
                     /locus_tag="PKZ52_16735"
     CDS             1771..2409
                     /locus_tag="PKZ52_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNG61472.1"
                     /translation="MLNTIALFSSIPAERLAQLEQHFNHRTYPKNTILFMEGDTAGQL
                     YIIESGMVSVYTDGADGKQLILNFLSDGDCFGELSLLDNKPRSASVMTLTDSKICSIT
                     REVFKAFLRENPDLYDALICELAGKVRQLTENVKDMALLDVYGRVAHTLERLTGDNNR
                     VTIPKLTHQDIANMVGASREMVSRIMKELVLGGYIELHQRHIEIKKSFPKGW"
BASE COUNT          665 a          699 c          618 g          547 t
ORIGIN      
        1 gtgaagggcg caactggtgg gcaacaggtg taaatccgcc tgggccgcta agcgcttttg
       61 aatcgccctg gcggttttgc tggcccgcag gcaggcgtcg gcgctgatat gggtatccaa
      121 ccaaagggcc aataaggcat cgcccacaat attgccgatg atgccgccgc tggcttttac
      181 ctcggcaatt aactcggcgt aataacgatt cattaattca tgtaattgcg caggcggtaa
      241 ttcttccgcc aggcgggtgt agccctgaat gtcggttaac agacacacac cactgaccac
      301 ctcattgtgc tgggcaaaat tactaatttg ctcgcttaag gccttggccg cctgggcggg
      361 catataatta cttaagtccg ttaacaaatt gtgatagcgc aatttattcg cccgtaagcg
      421 caaactaata tcgcagctgt aaaacaccaa actcaagaca aggggagcgg ccagcggcag
      481 gcggtaattc tgctgcacaa acgcccaata aactgccacc aggtacagcc cgtgccacag
      541 ggcgccagcc caccacacaa agcgcccagc gcggtgcgaa agcagccaca aaacgcccag
      601 ccacaccatt aacagcacag cctgctgcca ataggctgtg cgcattaaca tctgcttgtg
      661 taaaagattg cccaaggcgg tagcggaaat ttccacacca cttaaatcca aaccatctgc
      721 atcggaatac acggtgggat aagcgtcttg ttgttctgtt tgttggtttt ccaccaaacc
      781 cacgtacaca attttgccgc taaatgcgct gcgctgttca tccgccgaca tagttaacac
      841 ctggtcgatg ggccactggg gcaaatgtcc gggtgggccg taaaaattaa tggccacgcc
      901 atccgggtga ttaaggctgt gccacaagga taaacacagt gcctcagtac agctgcgcaa
      961 aaaggcctcg cgctcggccg aattaagtgc gtgtatctgc tgccaaatag gcagcgcgga
     1021 atttaaatta ggcgccgctt gctgcgcacg atctaaaaat aattgccagt cagcgggcct
     1081 aaccaggctt aaggctatta agggctggct gagtgtaaat tccccctgca tgtgcataac
     1141 ggggtaaaag tgatccacca agcctgccgc cttgggcaac acaaaaggcc cagatgcaag
     1201 ggccacttgt ttaaagcgcg ccagcggcgg gattatttct tctatatccg ctttgccgtc
     1261 atcggcgtgt ttactgccgc catcaacttg atagcgcttt aaataatcaa acaacaccac
     1321 gcggttggcc gctttaattg ctgcctctaa cgcggcatct tcctcttcat tgcgggcatc
     1381 cttaaaggcc acatcaaaaa caattaaggt cgcgcccgct gcagtaagct gattaattaa
     1441 ttgcgcgtgc actcggcgag aaaataaccg acgcggttgg ccgagggctt cagcggcctg
     1501 ctgattgagg gtaacgaggg caatttctga cgggggcacc aaagggccgc gccaggcaaa
     1561 caacacacgg cccgcctgct gctctgctag cggtagcaat gcaggcaaaa ctaggcacgc
     1621 aagcaaaccg cccaggacta ggagcaggtg cattttacgg ttacactgcc aagggtcacg
     1681 gccaacttgc atgagcacgc acgcattact gaaacaggga acgataccag aacccgtcgg
     1741 ccccttcccc cactaatttc ggtatacgcc atgctcaata ccattgcgct attttcgtct
     1801 attccagccg agcgcttagc tcagcttgag caacacttca atcaccgcac ctaccccaaa
     1861 aacaccattc tatttatgga aggcgacacc gccggccagc tgtacataat tgaatcaggc
     1921 atggtgagcg tgtacaccga tggcgccgat ggcaaacaat taattctgaa ttttttatcg
     1981 gacggcgact gcttcggcga attatccctg ctggataaca aaccccgctc cgcctcagtg
     2041 atgaccctta ccgacagtaa aatttgctct atcacacgag aagtatttaa agcctttctg
     2101 cgggaaaacc ctgatttata cgacgccctt atttgcgaat tagccggcaa ggtgcgccag
     2161 ctaacggaaa atgtaaaaga catggccctg ctggatgtct acggccgcgt cgcccacacc
     2221 ctagagcgcc taaccggcga caacaaccgc gttaccatcc ccaaattaac ccaccaggat
     2281 attgccaata tggtgggcgc ctcgcgggaa atggttagcc gcatcatgaa agaactcgtt
     2341 ttaggtggtt atatcgagct gcatcagcgg catattgaaa ttaaaaaatc atttcccaaa
     2401 ggttggtaac ccatacttca tcctaaaaac cgcagttgaa ctcgtagcga gggtgcctaa
     2461 ggcgctgaat atgagcgctt ttttgtcaaa atttggcgaa agcagtagtc accactacgg
     2521 tgagtcaaa
//
LOCUS       DAOQGS010000600         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26206_length_2529_cov_4.420776, whole genome shotgun sequence.
ACCESSION   DAOQGS010000600 DAOQGS010000000
VERSION     DAOQGS010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26206_length_2529_cov_4.420776"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKZ52_16740"
     CDS             complement(<1..810)
                     /locus_tag="PKZ52_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61473.1"
                     /translation="MSSLANPLRKNAYCARASLWRRNLCALSGCLVLSSSAWAMRVEL
                     IGPDSSTYTISEGQTLPLKLRASFENEEIAPGTECVATGRVVARRSSESAPVEGEDYT
                     LEGQAFSIKFSTPPQTQTAALFAPGVGEADFNIIALKDKLVEGVEQFQISAEEVVVNC
                     SGPNFYGGPITPYVPFMSYGPYVEITDATVPVDYEAEKLALLPELESITVLTMSGAQA
                     RNKTLAKQMDFARQKPGQNNRVSLKLDGAAIPVAGGNAGDDQVFEQVLAEGE"
     gene            1402..2325
                     /locus_tag="PKZ52_16745"
     CDS             1402..2325
                     /locus_tag="PKZ52_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNG61474.1"
                     /translation="MPSQQFRLARQIRRLLGFIFLVLLSGSFLAEAAPYQFANTSVQT
                     LKAATGDLSHQLIITLPDSYATEKTKRYPVVYYLDAYWDFPLLYATYGNLRYDRAIPE
                     VILVGLSIAGEGYNAYRSRYFSIFSDPHKKFLTGQANLLYRHLTQDIIPSIDAQLRTQ
                     ANPAGRILAGQSMGGLFTLFGLLQPSAAFGRFIAVNPAVAGSEAELARLQMARTETSL
                     NARLFISHGEAEYAPFRDPIIQFQQHLKARNYAGLYLSTQTLAGMGHTGGKGEAYAKG
                     LLWVLQDLAPAEKSGLQMDMEAEHKPAPAAK"
BASE COUNT          615 a          675 c          634 g          605 t
ORIGIN      
        1 ctcgccttcg gcaaggacct gctcaaatac ctgatcatcg ccggcattgc cgcccgccac
       61 aggaatcgct gcaccgtcta atttcaggct gacgcgatta ttctggccag gtttttggcg
      121 ggcaaaatcc atttgtttgg caagagtttt attgcgcgcc tgagcaccgc tcatggtgag
      181 cacggtgatg gattctaatt caggtaataa ggctagtttt tccgcttcat aatctacagg
      241 cacagttgca tcggttattt ccacataagg cccgtaggac ataaatggca catagggtgt
      301 aatggggccg ccgtaaaaat tggggccgct gcaatttacc actacttctt ccgctgaaat
      361 ttgaaattgt tcaaccccct caaccagttt gtcttttagc gcaataatat tgaaatcggc
      421 ttcaccaaca cccggtgcaa aaagggcggc ggtttgtgtt tgcggcgggg tactaaattt
      481 aatgctgaag gcttgccctt caagcgtgta atcctcgcct tccaccgggg cggattctga
      541 ggagcggcgg gccactacac ggccggtggc cacacattca gttcccgggg ctatttcttc
      601 gttttcaaag gaggcgcgca attttaacgg cagggtttgt ccttcagaaa tagtgtaggt
      661 tgaggagtcc ggcccgatta attcaacccg catggcccaa gcgctgctgc tgagcactag
      721 gcagccactg agggcgcaga gattgcggcg ccagagcgag gcgcgggcgc aataagcatt
      781 ttttcgtaat ggattagcta agctactcat aattacctcg tgtttattcc agatcgagtg
      841 ttacacatga gcgcctgcaa aacgccaaaa tccaggggca ttattcttag caaggagtgg
      901 gagcaggcag caaaaaacct cggcacttgt cggcctaagt cagttaatac agcgcaaagg
      961 atgcgagccc cctggtgact gaccggggtt cggcacaggg caaaggcaca cctatttcca
     1021 ctgcaaccac accaaccgag gcataacaga gcgtctgagg actaggccgg atgctaccac
     1081 aggccatggg gcaacaacac ctaagtctgg gcacacggag aaatgggtaa tttttcacct
     1141 aattgaacag gaaaattccc cgtttatgca cgcttcttta aaagtgtttt cctctggcct
     1201 cccggtgcac atgtgttgca gcgcgcagca aatagctgcg cgccagccgt ataattgccg
     1261 tgcatcaggc caatctattt ggccgccgaa ttaacacatc agctggctaa aacacctgca
     1321 aagaaagact aaaaaaattt atgcctgccc agattttttg ccgggcaagc aaatggtttc
     1381 ctctatctgg atcaattttt tatgccctca caacaatttc gcctggcccg ccaaatccgt
     1441 aggctcctgg gttttatttt tctagttctg ctcagcggct cgtttttagc tgaagctgcg
     1501 ccctatcagt ttgccaatac ctcggtgcaa accttaaaag ccgccacggg cgatttaagc
     1561 catcaattaa ttattaccct gccggattct tacgccaccg aaaaaacgaa acgttacccg
     1621 gttgtgtatt atttagatgc ctactgggat ttcccgctgc tgtatgccac ctacggaaat
     1681 ttacgctacg acagagccat tcccgaagtg attctggtgg gcttaagtat tgccggtgag
     1741 ggctacaatg cctatcgcag ccggtatttt tcgatatttt ccgacccgca caaaaaattc
     1801 ctaaccggcc aggcaaatct gttatatcgg catttaactc aagacattat tcccagcata
     1861 gacgcccaat tgcgcaccca agcgaacccg gccgggcgca ttctcgccgg gcaaagcatg
     1921 ggcggtttat ttacgctttt tggtttactg caaccatctg ccgcgtttgg ccgttttatc
     1981 gccgtcaacc cggcggtggc gggcagcgag gcggaacttg cgcgcctgca gatggcccgc
     2041 acggaaacgt cacttaatgc gcggttattt atttcccacg gcgaggcgga atatgcgccc
     2101 tttcgcgacc ccattattca gttccaacaa cacctaaaag cccgcaacta cgctggccta
     2161 tacctgagca cacaaacact tgcgggcatg ggccacaccg gcggcaaagg cgaggcctat
     2221 gccaaggggc tgttgtgggt attacaggat ttagcccccg ctgaaaaaag cggcctacag
     2281 atggatatgg aagcggagca caagccagcg ccagcggcta aatagccgcc aatctggcta
     2341 gtttggcagc gaaggccaac tatttttgtg ttaataaatt cacacatttc gccgtatacg
     2401 taaatttacg tacatacaag cgcagagccc ttgggcacag tggcgctggg ggtttcatcc
     2461 aagcgctccc aatacgacat aagagccggg taggtatagg taaggagcgc ctgcccggct
     2521 cttgacttg
//
LOCUS       DAOQGS010000601         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26309_length_2523_cov_3.641005, whole genome shotgun sequence.
ACCESSION   DAOQGS010000601 DAOQGS010000000
VERSION     DAOQGS010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26309_length_2523_cov_3.641005"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1186)
                     /gene="bamB"
                     /locus_tag="PKZ52_16750"
     CDS             complement(35..1186)
                     /gene="bamB"
                     /locus_tag="PKZ52_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNG61475.1"
                     /translation="MRQVLRAAAVALALCLAACSKVDENLKPVDLVKFDASVTLKKRW
                     SQSIGAGHDRFYNRFRVAVQGEHLFAADDKGGVFALNASNGKTLWKTQLDTAIGGAVG
                     ASPSSVLVGTLKGEVIALDASNGKERWRAKVSSSLVTPPQGTDAMVIALTNDNRLFAF
                     DAVTGQQRWTYDHSMPVLTYRTQATPVIVDDQVFVGFDNGQLLSFALSDGQLRWSTRV
                     AQPKGRTELNRMVDIDSTPIVDGPFVYAGSANGRLVAINRTTGRVSWGQNLSTLFDIA
                     KSGDLIFASGDDSHVLAFNAASGEPAWENKQLHRRNISAPGVLGEYVAVIDDEDYLHL
                     LNQKDGSFAGRMKPSGSEFRAPMLTLGDSLLIFGDDGTLSAYGLQPLKK"
     gene            complement(1183..1938)
                     /locus_tag="PKZ52_16755"
     CDS             complement(1183..1938)
                     /locus_tag="PKZ52_16755"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG61476.1"
                     /translation="MSAHLTEEEQIEAFKRWWQRYGTSTVASVALASALYVGWNLYQN
                     HRAEVAAEASKQYDKLAQLIKPLNDKPLSAEQKTQAKGLAEALVKDQKGSLYADLASL
                     ALAKLAVEDNDLATATTALRRVVETSTDAPTVDIARLRLAKVLAADKKVDEALGLLGA
                     AVNAEFEASYAEAKGDILVQENRLAEAFSAYQAAMTSVNKQGGGQESAMRRSILQFKL
                     ENAREPGTAVNPLAETPANSPAAEAAPAAEAKP"
     gene            complement(2005..>2523)
                     /locus_tag="PKZ52_16760"
     CDS             complement(2005..>2523)
                     /locus_tag="PKZ52_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNG61477.1"
                     /translation="HRLVRGLDYYSDTVFEWVTDDLGAQGTVCAGGRYDGLVAQLGGR
                     PTPAVGCAMGVERLVLLLQTLEKIPEGISDDTDAVFIVLGDYMPQALALAESLRDEMP
                     LLRLTLQAGGGSLKNQLKRADKSSAQVALILGEAEFNCQQLVVKHLRQERAQAQVAFS
                     EAAALIEQWLTE"
BASE COUNT          511 a          767 c          705 g          540 t
ORIGIN      
        1 ggcggggtaa gcaatactga ctcaggctcg cactttactt ttttaacggc tgcaaaccat
       61 aggcagatag ggtgccatca tcgccgaata tcaacaggct gtcgcctaaa gtcagcatgg
      121 gcgcgcgaaa ctcgctgccc gatggcttca tccgccccgc gaagctgccg tctttttggt
      181 tgagcaaatg caggtaatcc tcatcgtcga tcaccgctac atactccccc aatacacccg
      241 gtgcactgat gttgcggcgg tgtagctgtt tattctccca ggccggctcg cccgaggcgg
      301 cattaaatgc cagcacatgg ctgtcatcgc cgctggcaaa aatcaggtcg ccacttttgg
      361 caatatcaaa cagggtagat aaattctgcc cccagctgac gcggccagtg gtgcggttaa
      421 tggccaccaa gcggccattg gcggaaccgg cgtagacaaa gggcccgtcc acaatcggcg
      481 tgctgtcgat atccaccata cggtttaact cggtgcggcc cttaggctgc gcaacccttg
      541 tggaccaacg cagctggccg tcgctaaggg caaagctgag caactggccg ttatcaaaac
      601 cgacaaacac ctgatcgtcc acaatcacag gggtggcctg agtgcggtag gtgagcaccg
      661 gcatgctgtg atcataggtc cagcgctgct ggccggtgac ggcatcaaag gcaaataggc
      721 ggttgtcgtt ggtgagggcg atcaccatgg cgtcagtgcc ctggggcggc gtcaccaggg
      781 agctgctcac cttggcccgc cagcgctctt taccattgct ggcatccagt gcaatcacct
      841 caccctttag ggtacccacc aacacactgc taggggatgc ccccacggcg ccacctatgg
      901 cggtatccag ctgggttttc cagagggttt tgccgttgct ggcattgagg gcaaatacac
      961 cgcctttatc atcggcagca aacaggtgct cgccctgcac agccacgcga aaacggttgt
     1021 agaagcgatc atgacccgcg ccgatggatt ggctccagcg tttttttaag gtcacggagg
     1081 cgtcaaactt aaccagatcc accggtttga ggttttcatc caccttactg caggcggcga
     1141 ggcacagcgc caaggccacc gccgccgctc ttaacacttg cctcatggct tggcctcagc
     1201 ggcaggagcc gcttcagcag caggggaatt ggcgggggtc tccgccagag gattcacagc
     1261 ggtgccaggc tcgcgggcat tctccagttt gaactgcaaa atactgcggc gcatggcgct
     1321 ctcctggccg ccgccttgtt tgttcacgga agtcatagcc gcctgatagg cgctgaaggc
     1381 ttccgctagg cggttctcct gcaccagaat atcgcccttg gcttcggcgt agctggcttc
     1441 aaattcggca tttaccgccg cgcccaagag acctaaggct tcatctactt ttttatccgc
     1501 cgccaacacc ttagctaaac gcagccgcgc tatatccacg gtaggggcat cggtgctggt
     1561 ctccaccact cggcgcagag ccgtggtagc ggtggcaagg tcgttgtctt ccacggccaa
     1621 tttggccagg gccaaggagg caagatccgc gtacaagctg cctttttgat ccttaaccag
     1681 cgcttcagct aagcccttgg cctgggtttt ttgctccgca ctgaggggtt tgtcattcag
     1741 gggcttaatc aattgggcga gtttgtcgta ctgcttagag gcctcggcag ccacctccgc
     1801 gcggtggttt tggtacaggt tccagcccac gtaaagggcg gacgctaagg ccaccgacgc
     1861 cacggttgag gtgccatagc gctgccacca gcgtttaaac gcttctattt gttcttcttc
     1921 tgtgagatgt gcagacacag tgcactccta ttatcgttat ggttaataaa tggaagcccg
     1981 gtgctttagg gcgccgggta ttggttattc agtgagccac tgctcgatta aagccgccgc
     2041 ctcgctaaac gccacttgcg cttgcgcgcg ctcctggcgc aggtgtttaa cgactagttg
     2101 ttggcaatta aattccgcct cacccaaaat cagcgccact tgggctgagg atttatccgc
     2161 ccgcttaagc tggtttttca agctgccgcc gccagcttgc agggttaggc gcagcagggg
     2221 catctcatcc cgcagggatt ccgccagggc caaggcttgc ggcatgtaat cccccaacac
     2281 aataaacacc gcatccgtat cgtcgctgat gccctcgggg atcttctcaa gggtttgcag
     2341 cagcaacacc aagcgctcaa cccccatggc gcagcccacc gcaggagtcg ggcggccacc
     2401 caactgggcc accaggccat cgtagcggcc accggcgcac acagtgccct gggcgcctag
     2461 gtcgtctgtc acccactcaa acactgtatc cgagtaataa tcgaggccgc gcaccaggcg
     2521 gtg
//
LOCUS       DAOQGS010000602         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26337_length_2521_cov_4.080697, whole genome shotgun sequence.
ACCESSION   DAOQGS010000602 DAOQGS010000000
VERSION     DAOQGS010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26337_length_2521_cov_4.080697"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PKZ52_16765"
     CDS             complement(<1..1256)
                     /locus_tag="PKZ52_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HNG61478.1"
                     /translation="MIFPFPVTNLSKALFSSCFAFALCALAGCDGGVLKFTAGTSASV
                     KVPENTDGKVWQASVVLDGPGSLTGLSYRLSGPDQDRFSINTATGEVSLREAADFEQP
                     GDADKNNEYRVTIEASANKQTVVQSLQVQVTDVSLPKVELVQPKLNENIAKGADFTAQ
                     AVVRLYDAESNQTLSGGAVYLNGQALQQDAKDTQLFTGILSVPTVGIDISLVSTFDGG
                     HSISAQGKIFNKLSALSPDIFGVNPGFYLFYLDKTDASVSKMNLANGISQHYVQDRAL
                     ASVGAIFDFNSSYQTAYLAVEEGVGASLKSHLYAFPVGKAIPKVFSAGCTTDKILNLA
                     YDGLNKRVLLVAQDNQASPGGYSVLAIGVDEANGFVNGEYMGDCKKALEAGIVWRIPE
                     SVIKGKFKQFAFHRVSGTFVLADERM"
     gene            2209..>2521
                     /locus_tag="PKZ52_16770"
     CDS             2209..>2521
                     /locus_tag="PKZ52_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61479.1"
                     /translation="MAVNSCLKMIGLAALMAFGSLCANAQSLNQGAYVPGTTTYQYGY
                     NSIPNIQISGAPADTDFKRSAMLHDGSTYRLYFFKKNSTNTLYQFGWNGSVYQYGYNS
                     IP"
BASE COUNT          776 a          678 c          459 g          608 t
ORIGIN      
        1 tgcatacgct catctgccaa tacgaatgtg ccgctaacac gatgaaatgc aaactgctta
       61 aatttacctt taattacact ctctggaata cgccaaacaa tgccggcctc cagggccttt
      121 ttacaatcac ccatatactc accattaaca aaaccattag cctcatccac tccaatcgct
      181 aacactgagt aacctccagg ggatgcttgg ttatcctggg ccaccaacaa tactcgttta
      241 ttgaggccgt cgtaggcaag atttaaaatt ttatcggtag tacaacccgc actgaagact
      301 tttggaattg ctttgccaac ggggaatgca tacaaatgcg acttaaggga agcgcctacc
      361 ccttcctcga cggccaaata ggcagtctgg taagaagaat taaaatcaaa tatagcacct
      421 acactagcaa gagcccggtc ttgaacatag tgttgggata tgccattagc caaattcatt
      481 ttagatactg aagcatcagt tttatcaaga taaaaaagat aaaaacctgg attaacgcca
      541 aaaatatccg ggcttaatgc cgacaattta ttaaatattt taccctgcgc acttatacta
      601 tgcccgccat caaatgtact gaccagcgaa atatctattc caactgttgg cacacttaaa
      661 ataccggtaa ataattgtgt atccttggca tcttgctgca acgcttggcc atttaaatac
      721 accgccccgc cactaagcgt ttgatttgac tccgcatcgt aaagccgcac cacggcctgc
      781 gcagtgaaat cagctccctt agctatattt tcatttagct taggttggac taattcaacc
      841 tttggcagtg aaacatccgt aacttgaact tgcaggctct gcaccacagt ttgtttgttg
      901 gcagaagctt caatagttac cctatattcg ttatttttgt ccgcatcccc cggttgctca
      961 aaatccgcag cctcacgcaa ggatacttcg ccagtagcgg tattaatgga gaacctatct
     1021 tgatcggggc cactgagcct ataactcaag ccggtaagac ttccaggccc atcaaggact
     1081 acactcgctt gccacacttt tccgtcggta ttttcgggca ccttaacact ggcactggtg
     1141 cctgcggtga atttaagtac tccgccgtcg caacccgcta gcgcacatag tgcaaatgca
     1201 aagcaagagg agaagagggc tttggacaaa ttggtaacgg ggaaagggaa aatcataaaa
     1261 ggctccagcc tggcaaactg aagcctgaac ttaatcaact agccttggta cttaaatacg
     1321 taaatttacg tattggtacc gcgatgcgaa gcaagtcgca caatttgcct ccctatttca
     1381 gaggcaagcc cgcccaaaag taatttatgc aagaggctca acatataaac tgcagataag
     1441 caccaacagg cacgggcgat acgccctcta tgcgcaggca tacccagccc aggcacacct
     1501 ttcacccttg ggttaaccgc acgccccgta ccattcgacc cagtgcgccc cattacaatt
     1561 ttttacaaat tagacttcta ctcaccctag catcaggccg aagattaaac ttcagcccat
     1621 tttccgcagc cccgcggctt gaggccttaa gaacagctcc ctggcaagca ccagccataa
     1681 agaagacacc tgagcctaga caccagattc gaaaccaata aaagccaatt agaaccaata
     1741 agtgagtctt caattaatag atgttttcaa aaaattaccg acggaaagcc tgcgcaccaa
     1801 acaattaaat gcactccaga tggcccgaca ttttcacgca gaatcaatta aaaaacccac
     1861 cccacctaat aataaaaaac tcaccatcaa aacaaataat tttaaaaaac agccaccctc
     1921 ttaaaccggc tagatcaatt tatacaaaca atccataaca cttccaccct agggttgaat
     1981 cctaaggaaa aagacggcat aaaccccaca cgcctcaagc ctctgactgc agaattattc
     2041 tcaccaccgt tgcacccatc acaataacgc gttaacctat ttgcaataac cccatagacc
     2101 caatttttat gggcttatta caaggcaaag cccgctgttc ataaatgtgc aaccttcgcc
     2161 aaagacagtc gcactgcatc aacttaaccc attaactgag gacataccat ggccgtgaat
     2221 tcatgtttaa aaatgatagg attagccgct ttaatggcat ttggcagcct gtgtgccaat
     2281 gcccaaagcc tcaatcaagg tgcttatgtg cctggcacca ccacttatca atacggttac
     2341 aactcaatcc ccaatataca aatctcgggc gcacctgccg atacggattt taaacgcagc
     2401 gccatgctgc atgatggcag cacgtataga ttgtattttt ttaagaaaaa ctccaccaat
     2461 acactctacc aattcggctg gaatggcagc gtgtaccaat acggttataa ctcgatccct
     2521 a
//
LOCUS       DAOQGS010000603         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26477_length_2513_cov_4.101709, whole genome shotgun sequence.
ACCESSION   DAOQGS010000603 DAOQGS010000000
VERSION     DAOQGS010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26477_length_2513_cov_4.101709"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..641)
                     /locus_tag="PKZ52_16775"
     CDS             complement(24..641)
                     /locus_tag="PKZ52_16775"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG61480.1"
                     /translation="MTADQTADLIVIGSGPAGQKAAIQGAKAGQSVILVESSRELGGS
                     CVQRGTIPSKTLRENALRVKNMRLNAQLANYKLPEDIELNTLINRLSEVLAAHDTYMR
                     KQVERNGVTCIHGKAAFITPHSLNITRVRGGNLIVEAKKIIIATGSIPRKPDNIEVDH
                     EHIFDSDSILSMLYLPRSLTVLGGGVIASEYASIFQALGVKVTPP"
     gene            complement(638..1519)
                     /locus_tag="PKZ52_16780"
     CDS             complement(638..1519)
                     /locus_tag="PKZ52_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNG61481.1"
                     /translation="MPRLVSAMETSVVAKTIAQQLANKIKQKKIDVPMLPEVANRVMR
                     LTQDPNAITTDLAKLVQSDQVLAGHVMRIANSALYSPNTKLVSLQQAISRLGMSIIGE
                     IALAASINSKMFNAPGHNKIIQCQLRYALYTALWAKEVARVCKRNVEAAFLAGLLHDI
                     GRPVCLQATVEIATQCGFSVTNADLVPIEAAFSRALGVLVVNLWEMPAIVCDVLSMFD
                     RYEEAGDNKELTMVVVAASKFAQYGLSINSDQPTYSRKELEEQPVMADLNLYLEDIEF
                     LLGKQANVKSTLESMSL"
     gene            1832..>2513
                     /locus_tag="PKZ52_16785"
     CDS             1832..>2513
                     /locus_tag="PKZ52_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNG61482.1"
                     /translation="MYHVLVLGKQISTVANQVIAYFKGDAITDIGQLGDALEEKNYRI
                     VVYDGLADSTLDLPYCQEIATFLSPKGLPLLVLTNGVGPEEKLKAFEVGCDDLIDINL
                     ATVDEINARITKSIFHRIANEQLQKRLEMATETARNAMVDNSDLGANIQFLLAVNDCD
                     NLDQVGQQFFSTIERYGLRCSLQMRSTLGIKDMEAHGMAKDLESQLLFQLKDSSRYID
                     FGSRTIVNY"
BASE COUNT          616 a          570 c          628 g          699 t
ORIGIN      
        1 agttacttgg gatcaggaga aggttacggg ggcgtaactt taacacctaa agcctggaaa
       61 attgacgcat attcactagc gataacaccg ccgccgagca ccgtaaggct gcggggcagg
      121 tataacatgg ataaaataga gtcgctatca aatatgtgtt cgtggtccac ctcgatatta
      181 tcgggtttgc gcggaatcga gccggtggcg ataataattt ttttggcttc aacaattaag
      241 ttgccgccgc gcacacgcgt aatgttaaga ctgtggggtg tgataaatgc cgctttgcca
      301 tggatgcagg taacaccatt gcgctccact tgtttgcgca tataggtatc gtgggcggct
      361 aatacttcgc ttaaacgatt aatcaatgta ttaagttcaa tatcttcggg taatttataa
      421 ttggccagct gggcatttag ccgcatattt ttaacccgca gggcgttttc ccgcagggtt
      481 ttggagggaa tagtgccccg ctgcacacag gagccgccca gctcccggct gctttccact
      541 agaataacgg actgcccagc ttttgctcct tgaattgccg ccttttggcc cgcaggcccg
      601 ctgccgataa caattaaatc agctgtttgg tcggccgtca taagctcatg ctctccaggg
      661 tgctcttcac attggcctgt ttgccaagca aaaactctat atcttctaga tataaattta
      721 aatcagccat aactggctgt tcttctagtt ctttacggct ataggtaggt tgatctgaat
      781 taattgataa gccgtactgc gcaaatttgg aggcggccac aaccaccatg gttaattctt
      841 tgttatcgcc cgcttcctcg tagcgatcaa acatgcttaa cacatcgcac actatggcgg
      901 gcatttccca taaattaacc accagtacac ccaaggcgcg gctaaacgct gcttcaatag
      961 ggaccagatc ggcgttggta acggagaaac cgcattgggt agcaatttct accgtagctt
     1021 gcaggcacac agggcggcct atgtcgtgta gaagccctgc taaaaatgcc gcttccacat
     1081 tgcgtttgca tacacgggcg acttctttcg cccataaggc ggtgtagagt gcgtagcgca
     1141 attggcattg aataatttta ttgtgcccag gcgcattgaa cattttgcta ttaatagaag
     1201 ccgctagagc aatttcgcca ataatactca tgcccagccg cgaaattgcc tgctgcaggg
     1261 aaacaagctt ggtgttgggc gaatagagcg cggaattggc aatgcgcatc acgtggcctg
     1321 ccagtacttg gtcgctttgt accagttttg ctaaatccgt tgtgatggca ttggggtctt
     1381 gcgttaggcg cataacccgg ttggccactt caggaagcat gggcacatcg attttttttt
     1441 gctttatctt gttcgccaac tgttgtgcga tggttttggc gacaactgaa gtttccatag
     1501 cagatactaa ccttggcatt aaatacgctt ctctatcaat ttagacgaga tcctattggc
     1561 gtgctgaaat tctttcccac cgcaagaggg ggttagcccc agcttgccgc tatggctaac
     1621 ccggccttaa tgaacttgct catctatatc tgtagctcgc caaaccggct aattccggct
     1681 gcgagcttag acccttgaag ccttggagtg gccttgcggc ccattggaca agcccacagt
     1741 ctcagctaac cttaagctag ggagcgctgc aggcctggcg ttcatgatta gggtagggtt
     1801 tctgaggatc tagcgcccga gggagaagaa tatgtaccat gttttagtcc taggtaagca
     1861 aatttccact gttgccaatc aggttatcgc ctattttaaa ggcgatgcga ttactgatat
     1921 tgggcagctt ggtgatgcgt tagaggaaaa gaattaccgc atagttgttt atgatgggct
     1981 ggccgattcc acgcttgatt tgccgtattg ccaggagata gcaacgtttt tatcgccaaa
     2041 aggattaccg ctattggtgt tgaccaatgg tgttggcccg gaggaaaaat taaaagcctt
     2101 tgaagtgggc tgtgatgatt taattgatat taatcttgcc actgttgatg aaataaatgc
     2161 ccgcattact aaaagcattt ttcatcggat tgctaatgag cagttacaaa aacgcttgga
     2221 aatggccacc gaaacagctc gcaatgcaat ggtggataac tcagatctgg gcgctaatat
     2281 ccagttctta ttggccgtga atgactgcga taacttggat caagtagggc agcagttttt
     2341 tagcactatt gagcgctacg gtttgcgctg cagtttgcag atgcgcagta ccttaggcat
     2401 taaagatatg gaagcccatg ggatggcgaa ggatttggag tctcagttgt tatttcagct
     2461 gaaggattcc agccgttata ttgattttgg ttcccgcacg attgttaact atg
//
LOCUS       DAOQGS010000604         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26615_length_2503_cov_4.958742, whole genome shotgun sequence.
ACCESSION   DAOQGS010000604 DAOQGS010000000
VERSION     DAOQGS010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26615_length_2503_cov_4.958742"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKZ52_16790"
     CDS             <1..396
                     /locus_tag="PKZ52_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61483.1"
                     /translation="SAYLELTGDDLLAAGVAIMARPPGKKNSTIHLLSGGEKALTALA
                     LVFALFRLNPAPFCLLDEVDAPLDDANVGRFARLVEAMAEQVQFIFITHNKIAMEMAE
                     HLLGVTMQEPGVSRVVSVDIAHAAELAAD"
     gene            411..1400
                     /gene="zipA"
                     /locus_tag="PKZ52_16795"
     CDS             411..1400
                     /gene="zipA"
                     /locus_tag="PKZ52_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HNG61484.1"
                     /translation="MREWLIIISVLLIAGILLDGWRRMRAPKIKMARQEVSANDALPL
                     DEDPPLSSEFPSGGARIAAVRDDNDAENILQNVRQKHVESKKTRGVRKEGAEPDVIEK
                     VAPEPAADPLPNLMKAFAKRTQAPQVSTQVPSGDDGFIAPDLKKLDAELDDLFPEIKP
                     ISVKAEAKAELKPAAAKAAPQAAPAPQEVLVMHIMAPKGSTFYGDDLLPILMDLHMRF
                     GDMDIFHRHAQENGTGPVLFSLANLVKPGTFELSNMNDFETPGLSLFMTLPCGEEAMH
                     AFALMAQAAKRLTQGLGGELKDEARNNMTQQIFEHYKERVAEFERKWRLQKAH"
     gene            1481..>2503
                     /gene="ligA"
                     /locus_tag="PKZ52_16800"
     CDS             1481..>2503
                     /gene="ligA"
                     /locus_tag="PKZ52_16800"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNG61485.1"
                     /translation="MDQATARARVAELTQLITQANQAYYVQDDPSIPDAEYDRLFREL
                     KTLEEQFPELRTAFSPTQRVGAPPLKSFAEVSHFVPMLSLDNAFSQEDLWAFDKRIKD
                     RLKSTAELAFCCEPKYDGIAVSLLYEAGILVRGATRGDGAVGEDITQNVRTIRSIPLR
                     LSGDLIPARIEVRGEIYMTKTGFAAMNEQARQNGEKLFVNPRNAAAGSLRQLDSRITQ
                     TRPLFMCAYAVGLVEGGAVADEHFALLQQLKSWGFAISDEVTLADNIDACWDYYQRIM
                     AKRAQLPFDIDGVVFKVNSGQLQQQLGFISRAPRWAIAHKFPAQEEITQLIDVDFQVG
                     RTGTITP"
BASE COUNT          616 a          649 c          651 g          587 t
ORIGIN      
        1 agtgcttatt tagaattaac cggtgatgat cttttagcgg ccggtgtcgc catcatggcg
       61 cgcccgcccg gcaagaagaa cagcaccata catttgttat ccggcggcga aaaagccctt
      121 actgccctgg cgctggtatt tgcgctgttt cgcctcaacc ctgcgccgtt ttgcctcctg
      181 gatgaagtag acgccccgtt ggatgatgcc aacgtggggc gttttgcccg tttggttgag
      241 gccatggccg agcaggtgca gtttattttt atcacccata acaaaatagc gatggagatg
      301 gccgaacacc tattaggtgt gaccatgcaa gagcccgggg tgtcccgtgt ggtcagcgtc
      361 gatatcgccc atgcagccga attagctgcc gattgaggaa tattagaaga atgcgtgaat
      421 ggctcataat tatcagtgtg ttattaattg ctggcatttt gttggacggc tggcgccgta
      481 tgcgcgcgcc aaaaattaaa atggcccgcc aggaggtctc cgccaacgac gccttgccct
      541 tggatgaaga tccgcccctc tccagcgaat ttcccagcgg cggtgcgcgc atcgccgccg
      601 tgcgcgatga caacgatgca gaaaatattc tgcaaaacgt gcggcaaaaa catgtggaat
      661 caaaaaaaac ccgcggtgtg cgcaaagagg gcgccgagcc cgatgtaatt gaaaaagtcg
      721 ccccggaacc ggccgccgat ccgctgccca atttaatgaa agcctttgcc aagcgcaccc
      781 aggcgccgca agtgtctaca caagtgccca gcggcgatga tggttttata gcgccggact
      841 taaaaaaact cgacgccgaa ttggatgatt tattccccga aataaaaccc atcagcgtaa
      901 aagcagaagc caaagcggaa ttaaaacccg ccgccgccaa agccgccccc caggccgcgc
      961 ccgcgcccca ggaggttttg gtgatgcata ttatggcgcc caagggcagc accttttacg
     1021 gtgatgacct actgcctatt ttgatggacc tgcatatgcg ctttggcgac atggatatat
     1081 tccatcgcca cgcccaggaa aacggcactg gcccagtgtt atttagcctc gctaatttgg
     1141 ttaaacccgg cacctttgaa ttatccaaca tgaatgattt tgaaacgccg gggttaagtt
     1201 tatttatgac cctgccctgc ggtgaagaag ctatgcatgc ctttgctttg atggcccagg
     1261 cggccaaacg cctcacccag ggtttgggcg gtgagctaaa agacgaagcg cgcaataata
     1321 tgacccagca aatatttgaa cactataaag agcgggtggc ggaatttgag cgtaaatggc
     1381 gtttgcaaaa ggcccattaa gccccagtgc gaatggcttt aatttatagg ttgaatttat
     1441 tacgccctgt ttaacagggc gttttttatc tgaggtgtgt atggatcagg caacggcaag
     1501 ggcgcgtgtc gcagagttaa cccagttaat tacacaggcc aatcaagcct attatgtgca
     1561 ggatgacccc agcattcccg acgcggaata cgaccgctta tttcgcgaat taaaaacctt
     1621 agaagaacaa tttcccgaat tgcgcaccgc tttttcgccc acccagcgag tgggcgcgcc
     1681 gcctctaaaa agttttgctg aagtcagcca ttttgtgccc atgttgtcct tggataacgc
     1741 ctttagccaa gaagatttat gggcctttga taagcgcata aaagaccgcc taaaatccac
     1801 cgctgagctt gcattttgct gcgagcccaa atacgacggc atagccgtta gcctgttata
     1861 cgaagctggc attttagtgc gcggggcgac tcgcggcgac ggcgcggtgg gggaggatat
     1921 tacccaaaat gtgcgcacca ttcgcagtat tcccctgcgc ttaagcggcg atttaattcc
     1981 agcgcgcatt gaagtgcgcg gtgaaattta catgactaaa accggttttg ccgccatgaa
     2041 tgaacaggcg cgccaaaatg gcgagaaatt atttgtcaat ccgcgcaatg ccgctgcggg
     2101 cagtttgcgc caactggaca gccgcatcac ccaaactcgg cctttattta tgtgcgccta
     2161 tgcggtgggt ttggtggaag gtggcgcggt ggccgatgaa cattttgccc tgctgcaaca
     2221 attaaaaagc tggggctttg caattagcga tgaagtcacc ctggcagata atatcgatgc
     2281 ctgttgggat tattatcagc gcattatggc taaacgcgcc caattgcctt ttgatattga
     2341 tggtgtggta ttcaaagtta actcaggtca gttacaacag cagctcggtt ttatctcccg
     2401 cgccccgcgc tgggccatag cccataaatt ccccgcccaa gaagaaatta cccaattaat
     2461 cgacgtggat tttcaagtgg gtcgcaccgg tactattacc ccc
//
LOCUS       DAOQGS010000605         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26727_length_2497_cov_3.553645, whole genome shotgun sequence.
ACCESSION   DAOQGS010000605 DAOQGS010000000
VERSION     DAOQGS010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26727_length_2497_cov_3.553645"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..736
                     /locus_tag="PKZ52_16805"
     CDS             380..736
                     /locus_tag="PKZ52_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61486.1"
                     /translation="MLQILFAALVSVFLASSVLACPGGDCPDGKCPLSKKLDLDPERA
                     AKLEALETQFKQDREAMQAAHQAKLAEILTPDELAKLAAAHGHHGGEGHHGAIGDEPK
                     KCDEKDKKTAAKKSPI"
     gene            825..1214
                     /locus_tag="PKZ52_16810"
     CDS             825..1214
                     /locus_tag="PKZ52_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61487.1"
                     /translation="MLKFFCISLSILLALDSAGCSSQRGFNAPDLQFKTLITADHIKR
                     FELTLIPKPAQLDPLSSRRGLEEAPSERQLKGLLKDTLASNGFCKAGFLPLGRYAGES
                     HSRLRGECKDAATPEDIAKFPNTIERW"
     gene            complement(1325..1667)
                     /gene="rnpB"
                     /locus_tag="PKZ52_16815"
     ncRNA           complement(1325..1667)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKZ52_16815"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1719..2378)
                     /locus_tag="PKZ52_16820"
     CDS             complement(1719..2378)
                     /locus_tag="PKZ52_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNG61488.1"
                     /translation="MKQLASLCVQSLLLVSLSAPVLANPVYWTDWTAVPTAASAEGSI
                     QVDNTLVGVTYTGSSNHAFVQTGSGTNYWGGNAYTQGSVDNAPLPSELVALNAGGAVT
                     ISFSQPVVDPYIGLVSWNGNTVNFGQPISIDSFGPGFWGTGTPVLNSAQTGFFGNGEV
                     HGVISLKGTYSALSFSHTSENWHGFTVGVSAVPWPASAWLFALGLGVLAPVARKATAG
                     R"
BASE COUNT          617 a          726 c          647 g          507 t
ORIGIN      
        1 taatgaaacc ccggataagc agcaaaccct tataccggaa attctattac cctcatagaa
       61 gtttcaagac agaagcatgc agatggatca caaattccat cgaaggcaac cccatactcc
      121 atggatcatg gttttagcga agactgatta ttgtagctgc atacggagcc aaagcagtca
      181 gtccggtttt tcttcacgaa cgtgacccag tcgccgttga agctctttgg ggcggcacca
      241 tactaaagct gctaacacgg ctcgcagcgc gcaccaacct gtgacctagg ttcaagcttg
      301 tacaatttgg cttgaatccc tggaacctga gcaggcccgt ggcaaccaat tcactcacct
      361 taccccactg gagttcacca tgttacagat cctttttgcc gctttggtta gcgtcttttt
      421 ggccagcagc gtattagcct gccccggcgg cgactgcccc gatggcaagt gccccctatc
      481 gaagaaactg gatttggatc ctgagcgggc tgccaaactt gaagcgttgg aaacccagtt
      541 caaacaggat cgcgaggcta tgcaggcggc gcaccaagca aaattagcgg aaattctcac
      601 ccccgatgag ctggctaaat tggcggcggc ccacggccat cacggcggcg aaggccacca
      661 cggcgccata ggggatgaac caaaaaaatg tgatgagaaa gacaaaaaga ccgccgctaa
      721 aaaatcccct atttaatcag ccgctgaagc agtagccctg cgtgataagc ttaggcggcg
      781 gcagcttcta cgctgccgcc attccaccct aaaacgagga ctgcatgctt aagttctttt
      841 gcatttcgct tagtatcctg ctcgcgctgg actcggccgg ttgcagcagc caacgggggt
      901 ttaacgcgcc cgatctgcaa tttaaaaccc ttatcaccgc cgaccacatt aaacgctttg
      961 agcttaccct aatccccaaa cccgcccagc tggatccttt atcgagccgc cgcggcttag
     1021 aggaggcacc atccgagcgg cagctgaaag gcctgctaaa agataccctg gccagcaacg
     1081 gcttttgtaa ggcggggttt ttgccgctgg ggcgttacgc gggggagagc cattcgcggt
     1141 tgcgcggcga gtgtaaggat gccgccaccc ctgaggatat agctaaattc cccaacacca
     1201 tagagcgctg gtaacacagc ccggctcgag tctaaacaag agtctcagac gaaaaaaagc
     1261 ccggcagtta cactgccggg cttttttgat tcctaaaaac cgcagcggct attaggttat
     1321 taataggcgg agtgggtcga taagccgggt tctgtcgcgg acaatcattc atctgggacc
     1381 tgtgtcacca caggcctcaa gcgacctacc cgaatccagc gcgggccgcg ccaatggatt
     1441 cctatttggt cttgctccga acggggttta cctagccgca gactgttacc agctgcgcgg
     1501 tgcgctctta ccgcaccatt tcacccttac cacacataaa tgcatggcgg tttattctct
     1561 gttgcacttt ccgtaggctc gcgcccccca ggcgttacct ggcgttctgc cctatggagc
     1621 ccggactttc ctcccctgtt gccaggagcg attgtctaac ccactcacgg cgcgcacctt
     1681 aacaaaagcg gcgggcaatt gcgaccgcag aaatagcctt agcgaccggc ggtggctttg
     1741 cgggccacgg gcgccaacac gccaaggccg agggcgaaca accaagcact ggcgggccag
     1801 ggcacagcgc taacccccac ggtaaagccg tgccagttct cgctggtatg gctaaaactt
     1861 aaggcactgt aggtgccttt caaactgata acaccgtgca cttcgccgtt gccgaaaaaa
     1921 cccgtttgcg cgctgttgag cactggagtg cctgtgcccc aaaagcctgg gccgaagctg
     1981 tcgatgctga tgggctggcc aaaattaacg gtgttgccgt tccaactgac caggccgata
     2041 tagggatcaa ccaccggctg ggaaaagctg atggttacag caccaccggc atttaaagcg
     2101 accagttccg acggcagcgg cgcattatcc acactgccct gggtataggc attgccgccc
     2161 cagtaattag tgccactgcc ggtttgtaca aaggcgtgat tgctgctgcc ggtgtaggtc
     2221 acccctacca acgtgttatc cacctgaatt gaaccctccg ccgaagcggc agtaggcacc
     2281 gccgtccaat ccgtccaata caccggattg gccaatacag gagcgctcag ggacaccagg
     2341 agtaatgact gtacacacag tgatgcaagt tgtttcataa gggtacctaa atgagaaaaa
     2401 ttaaagaggg aggtgaaaaa tacacacaag gtagaggcgc aaatgccgcc cgcaaactta
     2461 acacatgaac aacacagaga ctaccgaggg cagcgct
//
LOCUS       DAOQGS010000606         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26799_length_2493_cov_3.613208, whole genome shotgun sequence.
ACCESSION   DAOQGS010000606 DAOQGS010000000
VERSION     DAOQGS010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26799_length_2493_cov_3.613208"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..716
                     /gene="dcd"
                     /locus_tag="PKZ52_16825"
     CDS             147..716
                     /gene="dcd"
                     /locus_tag="PKZ52_16825"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNG61489.1"
                     /translation="MAIKSDRWIRRMAQEHGMIEPFEAGQVRFDGAGQKLISYGTSSY
                     GYDVRCANEFKIFTNVHSVTVDPKHFDESSFVDIQSDVCIIPPNSFALARTVEYFRIP
                     RSVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLF
                     FESDEICETSYGDRKGKYQGQTGVTLPKT"
     gene            1305..1562
                     /locus_tag="PKZ52_16830"
     CDS             1305..1562
                     /locus_tag="PKZ52_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HNG61490.1"
                     /translation="MDINEITKAMTAEIYQAFKTAVELRKWPNGERLTPEQTATCLQA
                     IIAWETLHVPMEERVGYIPPKEEPCADDSHIHTHETPIKWQ"
     gene            1579..>2493
                     /locus_tag="PKZ52_16835"
     CDS             1579..>2493
                     /locus_tag="PKZ52_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNG61491.1"
                     /translation="MSSVGVTTYLKDYQAPEYSILTTQLNFALFDDYTEVTAELSLKR
                     NGEHSAPLRLHGQQLKLISIELNGAPLSTEHYSLDDEFLTIHTVPAECTLTTRVHIEP
                     HKNTALEGLYRSRTMYCTQCEAEGFRKITFYLDRPDAMSSFTTRIEADKTAYPVLLSN
                     GNLIAQGALENGRHFATWQDPFKKPAYLFALVAGDLACVEDSFTTASGRAVTLKIFVE
                     EKDLDKCEHAMASLKNAMRWDEEVYGREYDLDIFMIVAVDDFNMGAMENKGLNIFNTK
                     YVLADSETATDLDYQNIESVVGHEYFHNW"
BASE COUNT          688 a          578 c          605 g          622 t
ORIGIN      
        1 cccgccgctg gttgccgatc ccaacagtgc ggtaagccgt atttaccgca gtattgccag
       61 gcaattaggc gcgcgcttat gggcgcaggc ccaacagggc gcgggcgccc caactattgt
      121 aattactaac gattaaggtt tttagcatgg caattaaatc cgaccgctgg attcgccgca
      181 tggcccaaga gcacggcatg atcgaaccct ttgaggcagg ccaagtgcgc tttgacggcg
      241 ccgggcaaaa attaatttcc tacggcacct ccagctacgg ctacgacgtg cgctgcgcca
      301 acgaatttaa aatttttacc aatgtgcatt cggtaacggt agacccaaaa cactttgatg
      361 aaagcagttt tgtggatatc caaagcgacg tgtgtattat cccgcccaat tcattcgccc
      421 ttgcccgcac ggtggagtat tttcgcattc cccgcagtgt gcttactgtg tgtttaggta
      481 aatccaccta cgcccgctgc ggcattattg tcaatgtcac gcctctagaa cccgagtggg
      541 aaggccacgt cactttagag ttttccaaca ccacgcccct gcctgcgaaa atttacgcca
      601 atgaaggtgt agcgcagatg ttgttttttg aaagcgatga aatttgtgaa acctcttatg
      661 gggatcgcaa ggggaaatat caggggcaga cgggagttac gctgccgaag acgtaataaa
      721 ttcggaccat ctcaagcgtt gctctgatga tgaccttgct tgcatagtcg atgtgattaa
      781 acgcgcaatt aattggtgat aatgataagt aatcaaatcg aaatatcaga ggacgagaga
      841 aaattattga ttgaaatgac cgattttggc gtgcctctgt atgaggtatt aagcatcgcg
      901 tgtccttctt atcgggggca cactggcagc aaacaaatag agatcgccag aaacttggtc
      961 ctggggctaa tggagaaggg gttggtaggc ctatgtcggc ttactccaga aaacaccaaa
     1021 gatcatgtgt atgaactaag tgaatccatc tttatgtctt gcgaagaggt taaaactcac
     1081 ttgatatcgc tcatcaattg ggagaaaagc acggactccc ttgattgcac ggtatcatat
     1141 gagttggccc cgacaagctt aggtgaaaag gtgttggacg aaatatttag tatgagacga
     1201 attaaccgat agttgtaatc gcagctagtt tatcgtgcat cgaaaatatc agttgcaaat
     1261 gcaggcgttg ccactttcaa agactgctta taggtagggt atcaatggat atcaacgaaa
     1321 tcaccaaagc catgaccgct gaaatctacc aagcgtttaa aaccgccgtt gagctgcgca
     1381 aatggcccaa tggggagcgc ttaacccccg agcaaacggc tacctgttta caggcgataa
     1441 tcgcctggga aaccctgcat gtgccgatgg aagagcgggt gggttatatt ccgccaaaag
     1501 aagaaccctg cgcggatgac agccatattc acacccacga aaccccgatt aaatggcaat
     1561 aattgcccga ggcctgttat gagtagcgtt ggcgtaacca cttatttaaa agattaccaa
     1621 gcccctgaat attctatcct caccacccaa ttaaattttg ccctctttga cgactacacg
     1681 gaagtaaccg ccgaattaag ccttaagcgt aatggcgagc actcggcgcc gttacgctta
     1741 cacggccagc agctaaaact tattagcatt gaattaaacg gcgcgccatt aagtactgag
     1801 cactacagcc tggatgatga atttttaacg attcacactg tgcccgctga atgtacgcta
     1861 acaacccgtg tgcatatcga gccccacaaa aatactgccc tagagggttt gtatcgctcg
     1921 cgcaccatgt attgcaccca gtgtgaagcc gaaggctttc gcaaaattac cttttattta
     1981 gaccgccctg atgccatgag tagttttacc acccgcattg aggcggataa aacggcttac
     2041 ccggtgctgc tctccaacgg caatttaatt gcgcaaggcg ccttagaaaa tggccgccat
     2101 tttgccacct ggcaggaccc gtttaaaaaa cccgcttatt tatttgccct ggtggccggt
     2161 gatttagcct gtgtggagga ttcttttacc accgcctcag gccgagccgt aaccttgaaa
     2221 atatttgtgg aagaaaaaga cctggataag tgcgagcacg ctatggcgag tttaaaaaac
     2281 gctatgcgct gggatgaaga ggtgtatggc cgcgaatacg atctggatat ttttatgatc
     2341 gtggcggtgg atgattttaa tatgggcgct atggaaaata aaggcctcaa tatttttaat
     2401 actaaatatg tgttagcaga cagtgaaaca gcaactgatc tggattatca aaatattgaa
     2461 agtgttgtgg ggcatgaata ctttcataat tgg
//
LOCUS       DAOQGS010000607         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26917_length_2486_cov_7.430687, whole genome shotgun sequence.
ACCESSION   DAOQGS010000607 DAOQGS010000000
VERSION     DAOQGS010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26917_length_2486_cov_7.430687"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..587
                     /locus_tag="PKZ52_16840"
     CDS             357..587
                     /locus_tag="PKZ52_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG61492.1"
                     /translation="MGRDYEQVRQDLARNMKDFRGKRGLSQEALALSADVDRTYVSQI
                     ERAVGNPSLLVLCKLGAILETDVLDLLTEQTK"
     gene            991..1551
                     /locus_tag="PKZ52_16845"
     CDS             991..1551
                     /locus_tag="PKZ52_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61493.1"
                     /translation="MKKQFLTLALALTAATATSGALAATQYLALAVNRNSIDTNSNHF
                     TISTDADTLEGIYGLEYNEFFAAEARLGLGINKGKRHLDGQEEALDLGEIKIGSMYAL
                     YAKPQFNSGAFKAFALLGYAGYNSEWSFRDEDYYASSNTAYKGISYGLGAGWSFGQHV
                     INIEWHRTKSKDTTIDSTAVSCQFNF"
     gene            complement(2130..>2486)
                     /locus_tag="PKZ52_16850"
     CDS             complement(2130..>2486)
                     /locus_tag="PKZ52_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61494.1"
                     /translation="VNMNAPSRQKKPAYRLNKLPMDFISIVIFKPPIAVEKNRSLSLK
                     TTVNNGRIYLLSVLAMKIKEEFKNCKNKKIVLFYNKIIFICADVWGGKKMGCVKRGLI
                     KDCAVLLVRRRCKSPG"
BASE COUNT          672 a          615 c          579 g          620 t
ORIGIN      
        1 gctgttgcac ggatgccagg agcgccgggc ggcgcgcttt ggtctggctt tgcttgattg
       61 gcaacgacgg ctgggcgagt ctcggtagta ggaggcggtg ccacgttacc ttggctggcc
      121 ttcgacacgc cacccaagga gaggtgaacc ttggtcgagc cgtcagcctg cggctgtgtc
      181 gtgacacagc cggtcacggt catcacaacg gccagcccgg ccagcatccg aaagcagagt
      241 ccactcatcc atcagctccc tttgatccca agttgagctg attatgaaga ctatattcga
      301 cattgtcgaa tatagtccgt ggattaaagg tctcatgacg cccatgctcg gcgttgatgg
      361 gacgagacta cgagcaagtg cgtcaggact tggcgcgcaa catgaaagac ttccggggca
      421 agcgtggctt gtcgcaggag gcgctcgccc tgagcgcgga tgtggatcgc acctacgtga
      481 gtcagatcga gcgcgctgta ggcaacccgt ccctgctggt cctgtgcaag ctgggcgcga
      541 tcctggagac cgacgtgctc gatctcttga cggagcagac caagtgaacg cgggtgaggc
      601 cacccgaatg gggatggccg caaaaaatgt atatggaagt gactacggaa tgagccaaac
      661 cgcgctgaga cccagtaaaa atggcacttc caaggccatc tttcacttta acgtctgata
      721 aggaacctct gaaaaatgta ggcggcgaag cgctggctag gcgaatttgc caaaaaaagc
      781 ggacgactgc agggaggcag gaggtagagc aatgcaggag caattgccga gtttacacgc
      841 agtaaatgag catttttgcg ggggcgccta gcctaaattc aacgacgcca gggcgagtaa
      901 ggtaattttt cagaatttcc ataagtattt aaccgcgatg gggctcataa cagaagcccc
      961 atttttttgg acacaccaag gagaacccaa atgaaaaaac aatttttaac gctagctctt
     1021 gcattaaccg cagctacggc aaccagcggc gcgctggccg caacccaata tttagccttg
     1081 gcagtcaacc gcaattccat cgataccaat agcaatcatt tcactatctc aactgatgcc
     1141 gatacgctgg aaggtattta cggccttgaa tacaacgaat tttttgccgc cgaagcgcgc
     1201 ttagggcttg gcatcaataa gggcaagcgc cacctggatg ggcaggagga agcgcttgac
     1261 ctgggagaaa ttaaaattgg ttccatgtac gccctgtatg cgaaacctca atttaattcc
     1321 ggggccttca aggctttcgc gttattgggt tatgccggtt ataactctga atggagcttt
     1381 agggacgagg attattacgc atcgtccaat accgcttata aaggcatcag ttatggctta
     1441 ggtgcgggct ggagctttgg tcagcacgtt ataaatattg aatggcatag gaccaaaagc
     1501 aaagatacta ctatcgatag cactgctgtt tcatgtcaat ttaattttta aacttaagga
     1561 agcactgaaa aattaatcct ttttaaagga tacgtcccaa aatcgaaaac tgtcaaaact
     1621 cagtcaaaaa tcgcctatca aattatcgtt ttccgcctgc gaattgccat tttatcaaat
     1681 cgctagcgca ctgcccctag ggcagcagag tctatttcat tcgtaataga atggttaatc
     1741 ccgttagcac atacagccca ttggtatttt tcgccaaacc gaaacagcga actgtgttgt
     1801 agtggaacac gcattatatg taaaaaacgc atgttccact ttggctcgaa caccgacttt
     1861 gatcttttcc gcctgcgcca atggattggt tttggccagt tttactcgcc agccaggtag
     1921 catagcgata aaccaatcga ccagtcgttt tgtatactct ttccgttttc acctccaata
     1981 taagccgcac caacacaaac aggttgctct tcgccgtgta ggaattttcc tgttcgcgca
     2041 atatccttac caatttttag taattattca gagtctctct aacaacgccc ccagaaaact
     2101 cttacacacc gacataaaaa gtgcctatat taacccggtg atttgcaccg ccgccggact
     2161 aacaacaccg cacagtcttt aattaacccc cttttaacac accccatctt ttttccaccc
     2221 caaacatcag cacaaataaa aataatttta ttgtaaaaca atacgatctt cttatttttg
     2281 caatttttaa attcctcttt gatcttcatc gcaagaacag aaagaagata aatccgacca
     2341 ttgttcacag ttgtttttag gcttaggctt cggttcttct ccaccgcaat gggcggctta
     2401 aaaataacga tcgagataaa atccatgggc aatttattca aacgatatgc aggctttttt
     2461 tgcctgctgg gcgcattcat attaac
//
LOCUS       DAOQGS010000608         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_26958_length_2484_cov_4.399753, whole genome shotgun sequence.
ACCESSION   DAOQGS010000608 DAOQGS010000000
VERSION     DAOQGS010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26958_length_2484_cov_4.399753"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKZ52_16855"
     CDS             <1..494
                     /locus_tag="PKZ52_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467203.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNG61495.1"
                     /translation="PHLARLAQKGERARFKFPRPMTDRPGLDFSFSGLKTFTLNTIEQ
                     HALADGLPDEQTCADIAAAFEEAVVDTLVIKSRRALEATGLKRLVIAGGVSANTRLRE
                     KLHSALQKVGGEVFYARHEFCTDNGAMIAYAGCQRLLRGAHAGLAVEVTPRWPLIDLP
                     VLA"
     gene            498..854
                     /gene="folB"
                     /locus_tag="PKZ52_16860"
     CDS             498..854
                     /gene="folB"
                     /locus_tag="PKZ52_16860"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNG61496.1"
                     /translation="MDWIFIRDLPLDTHIGVFAWEQAIEQRLFLDVDLAWDISLAAQT
                     DDLAHALNYAAVAERLQAWAQGYSCQLIERLADYLAQCLLREFNTPRVRLTLYKPGAI
                     PAARTLGVSIERWRDC"
     gene            856..1356
                     /gene="folK"
                     /locus_tag="PKZ52_16865"
     CDS             856..1356
                     /gene="folK"
                     /locus_tag="PKZ52_16865"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNG61497.1"
                     /translation="MQQVILGLGSNIEPRLHLGKAVVLLRRQFTDFVLSPIYETCAVG
                     FSGPNFWNLVAGVHTDLTLEELKAALRAIELTVGRPPQAQKWADRCIDIDILIYGNCR
                     GASVVGHLPRADILRHAYVLAPLADVYPDLCHLETGRSFAQHWRDFAGDKTGICGRFA
                     HELLFA"
     gene            complement(1495..1917)
                     /locus_tag="PKZ52_16870"
     CDS             complement(1495..1917)
                     /locus_tag="PKZ52_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNG61498.1"
                     /translation="MSLIPRNTLNEIEHLFDHTWLPFRYDGGRFGTLAPRVDLKEKDD
                     SFEITLELPGVKKENLAITLNAGILRIEAESKQESVEKNDKILRQERHYGKYVRSFEL
                     GPQVQDSDISATFENGILTLVAPKHKEVTPATRRIEVK"
BASE COUNT          537 a          604 c          665 g          678 t
ORIGIN      
        1 gcccccacct ggcgcgcttg gcgcaaaaag gcgagcgggc acgctttaaa ttcccccgcc
       61 ccatgactga ccggcccggt ttggatttta gttttagcgg tttaaagacg ttcaccctaa
      121 acacaatcga gcaacacgct ttggccgatg gcctgccgga tgaacaaacc tgcgcggata
      181 tagccgccgc cttcgaagaa gccgtggtgg ataccctggt gattaaatcc cgccgggcgc
      241 tggaggcgac gggattaaag cgtttggtca ttgccggtgg tgtgtccgcc aatacacgtt
      301 tgcgggaaaa actgcacagc gcgctgcaaa aagtgggtgg tgaggttttt tatgcccgcc
      361 acgaattctg cacggataat ggcgccatga ttgcctacgc cggttgccag cgcctgctgc
      421 gcggtgccca tgccggtctt gctgtggaag taaccccgcg ctggccctta atcgatttac
      481 cggtactggc ctaggttatg gactggatat ttattcgcga cttacccctg gatacacata
      541 tcggcgtgtt cgcctgggag caggccattg agcagcggtt atttttggat gtggatttag
      601 cctgggatat cagccttgcc gctcagaccg atgatttggc ccatgcactg aattacgctg
      661 ccgtggctga gcgcttacag gcctgggcac agggctatag ctgtcaatta attgagcgct
      721 tggcggatta tttggcgcag tgtttattgc gcgaatttaa taccccccgt gtgcgcctga
      781 cgctgtataa acctggcgcc atacccgctg cacgcacctt gggggtaagt attgagcgct
      841 ggcgggattg ctaaggtgca gcaggttatt ttgggtttgg gcagcaatat tgaaccgcgc
      901 ctgcatttag gcaaggcggt agtgctattg cgccgccaat ttaccgattt tgttttgtcg
      961 cctatttatg aaacctgcgc ggtaggtttt agcgggccga atttttggaa tttagtggcg
     1021 ggcgtgcaca cagatttaac gcttgaagaa ttaaaagctg ctttgcgggc tattgaattg
     1081 accgtggggc gtccacccca ggcacaaaaa tgggcagatc gctgcattga tattgatatt
     1141 ttaatttacg gtaattgccg tggcgcttct gttgtggggc atttgccgcg ggccgatata
     1201 ttgcgtcacg cttatgtgct ggcgccttta gctgatgtgt atccggatct gtgccacctg
     1261 gaaacgggcc gcagttttgc gcagcactgg cgcgattttg cgggggataa aacgggtatt
     1321 tgtgggcgtt ttgcgcatga gcttttgttc gcttaaaagc gcttgagcca tcaatcacat
     1381 atgtgcgaaa aacaaatttt cgtctgctat ttttgtgttc attatgtatg tcagcggcac
     1441 ctcagcccac cctgccgatt gcaggatggg ctgagattgt tgttgcgccg gtaactactt
     1501 gacttcgatg cggcgcgtgg ccggtgttac ttctttgtgt ttgggtgcta ctaaagtgag
     1561 tatgccgttt tcaaaggttg cggatatatc actatcttgc acttgcgggc ccaattcaaa
     1621 actgcgcaca tatttgccgt agtggcgttc ttgacgcaga attttatcgt ttttctccac
     1681 gctttcttgt ttgctttccg cctcaattct caatatgccc gcgtttaggg tgatcgccag
     1741 gttttctttt ttaacccccg gcagttccag tgtgatttca aaactatcgt ctttttcttt
     1801 taggtcaacc ctgggggcca gggtgccaaa gcgcccgccg tcatagcgaa acggcagcca
     1861 ggtgtggtca aacaggtgtt ctatttcatt gagtgtgttg cgtggtatta aactcatagc
     1921 ttagtctcct caaaaaaata attaactacg gggttggtat tattagggaa tctctaaacg
     1981 gcatcctcac cgcccttatt aactcggcac aaaaagccgc cggattaatg ctgtgcaagc
     2041 ccactgctgc ctgcctatgc aataggccgc caggtgatat tcgcagtatc ccttgcttgc
     2101 tcaagctgat atccccatgc ggctgtatcg tttaactgat ttaccgcttg ctgaatatag
     2161 tgtggctaac agggaaaaaa acaagttgtg ccaaatcaaa aaaatgcaga gatattaacc
     2221 cggcggcatt tagtgccggg ttaatgatcg cggcaaggat gccgcgctcg gcggtgcaaa
     2281 ccgccgcgag ctgcgttaaa acaagaaaat ccgcgcattt tcgccagttt taatgcggcg
     2341 acggcaaaat agatcaggac gatctatttt gccgccaggt taataagaca aaccctactt
     2401 ttgatctggc agcttgctgt ctagacaccg caatgaatac acatcatttc agacacgccg
     2461 tgaatacatc ctaagtaggc tcgt
//
LOCUS       DAOQGS010000609         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27036_length_2479_cov_3.819719, whole genome shotgun sequence.
ACCESSION   DAOQGS010000609 DAOQGS010000000
VERSION     DAOQGS010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27036_length_2479_cov_3.819719"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1840
                     /locus_tag="PKZ52_16875"
     CDS             <1..1840
                     /locus_tag="PKZ52_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNG61499.1"
                     /translation="IRASAAFELVSAPVLNILTYRYIPTELRNQLSHLSPQQQARINA
                     FNVRLQMRQRDTGEGFVSRTRLFHTLAGESIEVLRAVLANPLTTEADIQAVLAEQVQL
                     GEQLEREAAGLFDLFAERVLCSPHAIALYEGDQAISFARLAEKVTRISHQLRRAQVAP
                     GTIVGLASERSEAYMAGMLAIIQVGACFMPLDPSYPAERLRYMLADSAAPLVLVDAKG
                     AAALEDLCVRCMRVDFWLADTAACAIDLSAVKRSADDPLYVMYTSGSSGDAKGVVGLQ
                     GATLNRLHWMWRQFPFASDEVCCLKTSPSFVDSIWEMLGPLLAGIPAVIAHDSVIANP
                     AALVELLAKHRVTRLVLLPSLLKNLLNTTLNLAKRLPNLWLWTLSGEAVPTSLAADFR
                     IRLPQSSLLNLYGSTEVAADVTYWLLSPTTPVPATATLPIGAAIDQVQLYLLDENQNP
                     VPLGQEGELYVGGAALAQGYLHRPELTAARFLNLPQVAPGRLFRTGDRAWQMADGNWV
                     YSGRVDDLVKVQGVQVEPAEVERALLLMDEIVDAAVLAVGQEAVQLCAFVVLRGGKRL
                     SPEQIHNHLAHHLIAAAIPADIRFLTQLPRTPNGKIARVELAALYT"
     gene            2010..>2479
                     /locus_tag="PKZ52_16880"
     CDS             2010..>2479
                     /locus_tag="PKZ52_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNG61500.1"
                     /translation="MQCAQIRDAFLNFFASKGHTIVPSSSLIPGNDPSLLFTNSGMVQ
                     FKDVFIGNDKRPYTRATSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIFYAWEFLTQILKLPPERLWVTVHISDDEAADIWIKEVGVDPSRFSRLDED"
BASE COUNT          466 a          752 c          715 g          546 t
ORIGIN      
        1 gattagggct agcgccgcct ttgagctggt ctctgcgcca gtgctgaata tcctcaccta
       61 ccgttatatc cccacggagc tgcgcaacca gctcagccac ctaagccccc aacagcaggc
      121 gcgcatcaac gcctttaacg tgcgcctaca gatgcgccag cgggacacgg gggaggggtt
      181 tgtgtcgcgc acgcggctgt ttcataccct ggcgggtgaa tccattgagg tgttgcgggc
      241 ggtgctggct aaccccttaa ctactgaggc tgatatccag gcggtacttg ccgaacaggt
      301 gcagctgggg gagcagctag agcgggaggc ggcgggtttg ttcgatctat tcgccgagcg
      361 cgtactgtgc agcccccacg caatcgccct ttacgaaggc gatcaggcca tcagttttgc
      421 ccgcttggcc gagaaggtca cccgcatcag ccatcaactg cgccgcgccc aggttgcccc
      481 aggcactatt gtaggattag cctccgagcg ctcggaggcc tatatggccg ggatgctggc
      541 cattatccag gtgggtgcct gttttatgcc cctggatccc agttaccccg ccgagcgctt
      601 gcgctatatg ttggccgact cggcagcgcc cctggtgtta gtggacgcca agggcgccgc
      661 tgccctagag gacttgtgcg tgcgctgtat gcgggtggat ttttggctgg ccgataccgc
      721 cgcctgtgcc atcgacctca gcgctgttaa gcgcagcgcg gatgatcctt tgtatgtgat
      781 gtacacctcc ggctccagcg gcgatgctaa aggggtagtg ggcttgcaag gggccaccct
      841 caatcgcttg cactggatgt ggcggcaatt cccctttgcc agcgatgaag tctgctgcct
      901 gaaaacctcc cccagttttg tagactccat ctgggagatg ctcggccctc tgttggcggg
      961 cattcccgct gtcatcgccc atgactccgt gatcgccaac cccgctgctc tggtggagtt
     1021 actggccaaa catagggtta cccgcctggt tttactgccg agcctgctta aaaacctgct
     1081 caacaccacc ctcaacctcg ccaagcgcct gcctaacctg tggctgtgga ccctcagtgg
     1141 tgaggcagtg cctacgagcc tggcggcgga ttttcgcatc cgtttgccgc agagtagttt
     1201 gctcaatctc tatggctcca ccgaggtggc ggcggatgtg acctattggc tgctcagccc
     1261 cactacgcca gtgcccgcca cagctacact gcccattggc gcggccattg accaggtgca
     1321 actgtacctc ttggatgaga accaaaaccc cgtgcccctg gggcaggagg gagagctgta
     1381 tgtggggggc gccgccttag cccagggtta tttacaccgg ccggaattaa ccgccgcgcg
     1441 ttttcttaac ttgccccagg tggcacctgg gcggctgttt cgcacggggg atagggcctg
     1501 gcagatggcc gatggcaact gggtatacag cggccgggtg gatgacctgg tgaaggttca
     1561 gggcgtgcaa gtggagcctg ccgaggtaga gcgggcgctg ctgttgatgg atgaaattgt
     1621 cgatgcggcc gttttagcag tcgggcagga ggcagtgcag ttgtgtgctt ttgttgttct
     1681 gcgcgggggc aagcgcctga gccctgagca gattcacaac cacctggccc atcacttaat
     1741 cgctgcggct attcccgccg acataaggtt tttaacgcaa ctgccgcgca cccccaacgg
     1801 caaaatcgcg cgggtggagt tggcggcgct gtatacctaa aaaccttagt gaaaccgctg
     1861 ctgcgagcct ctgccaatag ggggggtgag gttttcgggc aacatcgcct tgcccgtcct
     1921 ggcagcctcc accacagcgc tttgccccaa gcctgcaacc ccttatactg cggccccttt
     1981 tcctttcggc tttgatgcgg aatctaccca tgcaatgcgc ccaaattcgc gacgcctttt
     2041 taaatttctt tgccagcaag gggcacacca tagtgcccag cagctctctg attccgggca
     2101 atgacccatc cctgttgttc accaactcgg gcatggtgca gtttaaagat gtatttatcg
     2161 gcaacgacaa acgcccctat acccgcgcca cctccagcca gcgctgtgtg cgcgccggcg
     2221 gaaaacacaa tgatttagaa aacgtcggct acaccgcccg ccaccacacc ttttttgaaa
     2281 tgctcggcaa cttcagcttc ggcgattact tcaagcgcga tgccattttt tacgcctggg
     2341 aatttctcac ccaaatcctc aagctacctc ctgagcgcct gtgggtgacg gtgcacatca
     2401 gcgatgatga agccgccgat atttggatta aagaagtcgg tgtagaccca agccgtttct
     2461 ctcggctcga tgaagacaa
//
LOCUS       DAOQGS010000610         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27051_length_2478_cov_5.096987, whole genome shotgun sequence.
ACCESSION   DAOQGS010000610 DAOQGS010000000
VERSION     DAOQGS010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27051_length_2478_cov_5.096987"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1170
                     /gene="lpxD"
                     /locus_tag="PKZ52_16885"
     CDS             139..1170
                     /gene="lpxD"
                     /locus_tag="PKZ52_16885"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNG61501.1"
                     /translation="MGSSKAFTLQALADYLGGRCIGEGSITLNRLAPLEAAGPGDLSF
                     VAKPALAKLLATAAASAYIVPPALADRVANGIEVAEPYVAYAKVSRLFEVCPQIPTGV
                     HPMACVDPSAILADDVAIGPFCVVAAGARIDAGVQLAAGVHIGARSHIGEGSYLFPNV
                     VIYHDVILGKRVRIHANTTIGSDGFGFAPSAQGWQKIHQLGGVIVGDDVEIGANTSID
                     RGALGATEIAAGVIIDNQVHIAHNVKIGAQTAIAGCVGIAGSAVVGAHCTMGGFVAIS
                     GHVEIADHVHFNGASVVTKSISEPGHYSSGVPLQEVKEWRRNAVRFSQLDSWVERIKA
                     LEQPKNEKI"
     gene            1189..1632
                     /gene="fabZ"
                     /locus_tag="PKZ52_16890"
     CDS             1189..1632
                     /gene="fabZ"
                     /locus_tag="PKZ52_16890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNG61502.1"
                     /translation="MMDILEIRKYLPHRYPFLFIDRVVELTVGESIVAYKNISINEGI
                     FEGHFPNFPVFPGVLILEAMAQACGVLGFKTMDKTPEDGSIYLYAGADDVRFKRQVVP
                     GDQLYLEAKILGEKRGIWKFECKAKVDGQLAASATIMCADRTVAN"
     gene            1647..2417
                     /gene="lpxA"
                     /locus_tag="PKZ52_16895"
     CDS             1647..2417
                     /gene="lpxA"
                     /locus_tag="PKZ52_16895"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNG61503.1"
                     /translation="MIHPSAIIDPAARIAEGVSIGPWTYIGPEVEIGPGCRIESHVVI
                     KGPTKMGHNNRVFQFSTLGEDTPDLKYRGEPTTLTIGDNNIFREGVTIHRGTVQDRGD
                     TLIGNGNLLMAYVHVGHDSVVGNHCILVNNAALAGHVVVHDWAILGGYTLVHQFCHVG
                     QHAFTGMGSAVGKDIPSYVMVTGSPAQARSINMEGLKRRGFSKDDIAVLNKAFKTVYR
                     RGLTLDDALIELQAMLPECAHLAPFIQSLENSSRGIVR"
BASE COUNT          552 a          631 c          699 g          596 t
ORIGIN      
        1 aaatgaaggc ggttattgat gagctggttg ccgctgaaaa aatcgatatt atcgtgaaga
       61 gccaagcggc actttgggcg gacccggctg ctgacatcac cgccaaagtc actgaaaagc
      121 tcaacaaagc taaataatat gggctcatcc aaggcgttta ctctgcaggc cctggcggat
      181 tatttaggcg gtcgttgtat cggtgagggt tctattaccc taaaccgcct cgcgccgctt
      241 gaagcggctg gccccggcga tttgtcgttt gtggccaaac ctgccctcgc caagttattg
      301 gccaccgccg cggccagcgc ctatatagtg ccgccagccc tggctgatcg ggtggcaaat
      361 ggtattgaag tggccgaacc ctatgtggcc tacgccaagg tgagccggtt atttgaagta
      421 tgcccccaaa ttcctaccgg cgtgcacccc atggcctgtg tggaccccag cgcgatactg
      481 gcagacgatg tggccatagg cccgttttgc gtagtggcgg cgggtgcgcg gatcgatgcc
      541 ggtgtgcagc tggccgcagg ggtgcacata ggtgcgcgca gccatatagg tgaaggcagc
      601 tatttattcc ccaatgtggt gatataccac gatgtgattt taggtaaacg agtgcgcatc
      661 cacgccaata ccaccatcgg ttctgacggt tttggttttg cccccagcgc gcaaggctgg
      721 cagaaaatcc accagcttgg gggggtgatt gtgggtgatg atgtggagat cggtgctaac
      781 accagtatcg ataggggtgc cttgggcgct accgagatag ctgctggtgt catcatcgat
      841 aaccaggtac atatcgccca caatgtaaaa attggcgcac agaccgctat tgcaggctgc
      901 gtgggtattg caggttccgc cgtggtgggt gcgcactgca ccatgggcgg ttttgttgct
      961 atcagcggtc atgttgaaat cgccgatcat gttcacttta atggagcgag tgtagtgacc
     1021 aagtctattt ctgaacccgg tcactattct tccggtgtgc cactgcaaga agtaaaagaa
     1081 tggcgccgta atgccgtgcg gttttcccag ttggattcct gggttgaacg tattaaggca
     1141 ttagagcaac ccaaaaacga aaaaatttaa taggcagagg cacttcctat gatggatatt
     1201 ttagagattc gcaaatactt accacaccgc tacccgtttt tatttattga ccgtgtggtt
     1261 gagttaaccg ttggcgagtc catcgtggcc tacaaaaata tctccattaa tgaaggtatt
     1321 tttgaagggc acttccccaa tttccccgtg tttccagggg ttttaattct tgaggccatg
     1381 gcgcaggcct gcggtgtgtt ggggtttaaa accatggata aaacccccga agatggctcc
     1441 atttacttgt acgcaggcgc cgatgacgtg cgttttaagc gccaggtggt gcccggtgac
     1501 cagctttatt tagaagccaa aatactcggt gaaaaacgcg gtatttggaa attcgaatgt
     1561 aaggcaaaag tagatgggca attggctgca tctgcaacta ttatgtgtgc tgaccgcact
     1621 gttgcaaatt aaattgaggt accggcttga tacatccctc cgccattatc gaccccgccg
     1681 ctcgcattgc agagggagtt tctattggcc cttggacgta tataggcccc gaggttgaga
     1741 tcggccccgg ctgtcgcatt gaatcccacg tggtcatcaa aggccctact aaaatggggc
     1801 acaataatcg agtttttcaa ttcagcaccc tcggcgaaga caccccggat cttaaatacc
     1861 gcggcgaacc caccacgctc accataggcg acaacaatat tttccgcgag ggcgtcacca
     1921 tccatcgcgg cacggtgcag gatcgcggcg acaccctaat tggcaatggc aacctgttaa
     1981 tggcttatgt gcatgtgggc cacgatagcg tggtgggcaa tcactgcatt ttggtcaata
     2041 acgctgcctt ggcgggccat gtggtggtgc acgactgggc cattctcggc ggctacaccc
     2101 tggtgcatca gttttgccat gtgggccaac acgcgtttac cggtatgggc agtgcggtgg
     2161 gtaaggatat tccgtcctac gtcatggtta ccggttcccc ggcccaggcc cgcagcatca
     2221 atatggaagg cctaaagcgg cgcgggttta gcaaagatga catagccgtg ttaaataagg
     2281 cctttaaaac ggtctatcgc cgtggcctta ccctggatga cgcgctcata gagctgcagg
     2341 ccatgctgcc cgagtgcgcg cacctggcgc cgtttattca gtcgcttgag aactcctccc
     2401 gtgggatcgt ccgttaacct cgctgccaaa cccctgcgca tcggtatggt ggcgggggaa
     2461 gagtcggggg atattctg
//
LOCUS       DAOQGS010000611         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27077_length_2477_cov_4.674649, whole genome shotgun sequence.
ACCESSION   DAOQGS010000611 DAOQGS010000000
VERSION     DAOQGS010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27077_length_2477_cov_4.674649"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKZ52_16900"
     CDS             complement(<1..497)
                     /locus_tag="PKZ52_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61504.1"
                     /translation="MIDWDKWQEIYLTLKKHKLRTALTAFGVFWGIFMLVVLLGAGNG
                     LKNGVVGMFGGQANTVYMWSGAPTQIPYAGMQRGRKLALKDEDLVAIRQGCPELDVLT
                     GNNDLGRWDGAQYVVRGTKFGAFPTKGIQPANAKLKAYTTALGRFINDADIEEKRKVA
                     VIGTRV"
     gene            complement(494..1756)
                     /locus_tag="PKZ52_16905"
     CDS             complement(494..1756)
                     /locus_tag="PKZ52_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61505.1"
                     /translation="MLSIDSLQEIAHTLKQNKLRTLLTAFGVFWGIFMLILLLGAGKG
                     MQNGMESMFSSDLRDSIWINPRQTSLPYKGLSPGRTIQLTEQDIAAIRQKIPDVQFLS
                     AENPLGSARQADTVVSYQNKTGSFGVMGVADGYFKIKAYQDYQMGRRLNELDQLEERK
                     VAVIGTRVYEKLFDKNEDPLGKIINVNGVTYKVVGVFYDSGWEGRMSERVYVPLSGFQ
                     KTFGKGDQVSLIALSPKPGADGFALEEQVVTLLKERHKVAPDDKKAIFSWNLAKQTRQ
                     FAQMFAAIDAFIWFVGIGTLMAGIVGISNIMLITVKERTVEIGVRKALGATPRQIVGT
                     LLLESILVTSVAGYLGLVLGVALLEAVSYVLTSFNIKLQFFSRPEVDFAIAIKAIILL
                     VAIGAFAGFLPAWRAAKISPIEAMRAAK"
     gene            complement(1750..>2477)
                     /locus_tag="PKZ52_16910"
     CDS             complement(1750..>2477)
                     /locus_tag="PKZ52_16910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG61506.1"
                     /translation="LAPGLYTSLRIPMIELANIHKYYGEGERRLHVLKGINLSIAQGE
                     LVSIMGASGSGKSTLLNILGILDSHNEGRYRLNGIEIDHLTENKAAYLRNHLIGFVFQ
                     SFNLLPFKNAMENVALPLYYQKVSRKQRNLEAMALLEKVGLGQKALNMPNELSGGQKQ
                     RVAIARALAGKPKLILADEPTGALDSVTTEEIMRLFKEMHKEGKTVVIVTHEADIARQ
                     TERLIQLKDGLILADTQQARAQC"
BASE COUNT          626 a          663 c          555 g          633 t
ORIGIN      
        1 acccgggtgc caattacggc tactttgcgt ttttcttcta tatccgcatc gttaataaaa
       61 cgccctaacg ctgtggtata ggcttttaat ttcgcgttag caggctgtat acctttggtg
      121 ggaaaagcgc caaatttagt accgcgcacc acatattgcg cgccatccca acggcctaga
      181 tcattattgc cggtcaagac atccagctcc gggcagcctt ggcgaatggc caccaggtct
      241 tcatctttta atgccagctt gcggcccctt tgcatgccag cgtagggtat ttgcgtgggt
      301 gcgccgctcc acatatacac cgtattggcc tggccgccga acatgcccac cacaccattt
      361 tttaagccat tgcccgcgcc gagcaatacc accagcataa atatgcccca gaacacacca
      421 aaggccgtaa gcgccgtacg caatttgtgt tttttaaggg tgaggtaaat ttcctgccat
      481 ttatcccaat caatcattta gctgccctca tggcttcaat gggggaaatt ttagcagcgc
      541 gccaggccgg taaaaaaccg gcaaaggcgc ctatggctac cagcaaaata atcgctttaa
      601 tggcaatcgc aaaatccact tccgggcgag agaaaaactg caacttaata ttaaaactgg
      661 tgagcacata agacaccgct tccaacagcg caacgcccag taccaaccct aaatagccgg
      721 ccacggaagt aaccaaaatg gattccaaca acagcgtgcc aacgatttgc cgaggtgttg
      781 cgcccagcgc tttacgcacg ccaatttcca ccgtgcgctc ttttacagtg atgagcatga
      841 tattactgat gcccacaata cccgccatca aagtgcctat gccgacaaac caaataaagg
      901 catcaatggc ggcaaacatc tgggcaaatt gccgagtttg tttggccagg ttccacgaga
      961 aaatcgcttt tttatcatcg ggcgcaactt tatggcgctc ttttaataaa gtcaccacct
     1021 gctcttccag ggcaaatcca tcagcaccgg gtttaggcga gagcgcaatc aaggagactt
     1081 gatcgccctt gccaaaggtt ttttgaaaac cactcaaggg tacatacacc cgctccgaca
     1141 tgcgcccttc ccaaccgctg tcgtaaaaca cgcccactac tttataagtc acgccgttca
     1201 cattaattat tttgccgagc ggatcctcat tcttatcgaa taatttttca taaacgcggg
     1261 tgccgataac cgccactttg cgctcttcaa gttgatccaa ctcatttagc ctgcggccca
     1321 tttggtaatc ctgataggcc ttaattttaa aataaccatc ggcaacaccc ataacaccaa
     1381 agctgccggt tttattttgg taggacacca ccgtatccgc ctggcgggct gaaccaagcg
     1441 ggttctctgc cgataaaaac tgcacatcag ggattttttg ccgaatggcc gctatgtcct
     1501 gttcggttaa ttgaatggtg cggccagggg ataggccttt gtagggcaat gaagtttgcc
     1561 gtgggttaat ccaaatagaa tcgcgcaggt cgctgctgaa catggattcc atgccatttt
     1621 gcatgccctt gcccgcgcct aataataaga taagcataaa aattccccaa aaaaccccaa
     1681 acgccgttag cagcgtgcgc aatttatttt gcttgagggt atgggcaatt tcttgcaggc
     1741 tatcgatact tagcattgcg cacgcgcctg ctgagtatcg gcgagaatta aaccgtcttt
     1801 taactggatt aaacgctcgg tttggcgggc gatatccgct tcgtgggtaa caatcaccac
     1861 ggttttacct tctttatgca tttctttaaa taaacgcata atttcttcgg tggtgacgct
     1921 atctaaggcg ccggtgggtt catcggccaa aattaattta ggcttacccg ccagggcgcg
     1981 ggcaatggcc acccgctgtt tttgcccgcc ggataattca ttgggcatat tcaacgcctt
     2041 ttggcccagg ccgacttttt ccagcagcgc catggcttct aaattgcgct gcttgcggct
     2101 caccttttgg taatacagtg gcagggccac attttccatg gcgtttttaa agggcaataa
     2161 attaaacgac tgaaacacaa atccaattaa atggttgcgc aaataggccg ctttgttttc
     2221 cgttaagtgg tctatttcaa tgccgtttaa acggtagcgg ccttcattgt ggctatcgag
     2281 gatgccgaga atattcagca gggtagattt acccgaaccg gacgccccca taatcgacac
     2341 caattccccc tgggcgatgc tgagattaat gcccttaagt acgtgcaggc ggcgctcgcc
     2401 ctcgccgtag tatttgtgaa tattcgctaa ctcaatcatt ggaatcctta aggacgtata
     2461 cagacctggg gcaagct
//
LOCUS       DAOQGS010000612         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27195_length_2471_cov_4.940397, whole genome shotgun sequence.
ACCESSION   DAOQGS010000612 DAOQGS010000000
VERSION     DAOQGS010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27195_length_2471_cov_4.940397"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..323
                     /locus_tag="PKZ52_16915"
     CDS             108..323
                     /locus_tag="PKZ52_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61507.1"
                     /translation="MRRGVIQYGLFACALMCGGRGLAVLEVNLDAEGSDAEAFLRQHP
                     VQFSLAASPAAQCPKAFGLASARRLFN"
     gene            301..666
                     /locus_tag="PKZ52_16920"
     CDS             301..666
                     /locus_tag="PKZ52_16920"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNG61508.1"
                     /translation="MPAAYLIDHQGRIRHEHLGVKTSLAANLRAQLKQLLNEPTQPPL
                     NRADTMIDPWRLWRPALLTLLITGCTSVEPWQRGRLAKPQMAADVHSLQDNLRSHVHS
                     SREAAAAGDVTDGGGCGCY"
     gene            653..>2471
                     /locus_tag="PKZ52_16925"
     CDS             653..>2471
                     /locus_tag="PKZ52_16925"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNG61509.1"
                     /translation="MAVIEQNAKPVTDALTALRLAAMALPGLLMAAPASAGDNISFVH
                     EHYAEGERDLQGLSSKFQPIEVDSLLGAAHFSLTDEISAGLNLSQDTWSGATPMATAP
                     SSAHGNRIAPHSHTHTTTGASMEEGGHLHDAENDPTPSAPQAQTLTGASPYLYSALKL
                     DSNRRPLQTDLEGKVIGGVDNQLVHTLSSASPEVRQQFDLILRRQAAQSSLSLGGGLS
                     QERDFGAWFGHAKGSWDFNRKLTSLGAGISYTHSKTNALLDQDVVPHVYEPYMLTYER
                     RGDDSYNRSHSSSRLELGQYGPRLKGDKDDWGLSLSLSQILNPSAYLTADLGFTHSFG
                     YQANPYKAVEVGFIDPLKQKGQAGGVATANYVFDAELVALLEQRPDLRNQASVNLRYV
                     QYLGAFDAALHLGLRAYSDTWGVKAQSLDARWVQPLGKWTLTPRLRYYSQSKADFYTP
                     YLLTQQGLFAQVTDPVKGAIYIDTRNTSNGLRYFEDPTGTVTPAIDTNPASRKYGQPV
                     VGSHGRAIIEQGSGRAVNDQSLVDNLKQDSLPFDRSLLPQHYSSDARLSAFGTLSAGL
                     IASRKINKELSVEFSYEQLRHAGSLKFGGGGEGSYADFDA"
BASE COUNT          528 a          786 c          708 g          449 t
ORIGIN      
        1 tgggcgggcc attatctggg ccgttagggg ggtgcacaat gcgacaaaat gtcgcaggct
       61 gggactacag atagctgtta gcattggctt ttatgtgagc gcgcggcatg cgtcgagggg
      121 ttattcaata cggcttattt gcctgtgcac ttatgtgtgg cgggcggggt ttggcggtac
      181 tggaggttaa cctggatgcc gaaggctccg acgccgaagc gtttttgcgc cagcacccgg
      241 tgcagttcag cctcgccgca agccctgccg cgcagtgccc caaggccttt ggcctggcca
      301 gtgcccgccg cttatttaat tgaccaccag ggccgcattc gccatgagca tttaggcgtt
      361 aaaacaagcc tcgcagccaa cctgcgcgct cagctgaagc aactgctcaa tgaaccaaca
      421 cagccccccc ttaaccgagc cgacacaatg attgaccctt ggcgcttatg gcgccctgcc
      481 ctactcaccc tactcataac cggctgcacc agcgttgaac cctggcagcg cgggcgcctg
      541 gctaagccgc aaatggcggc ggatgtgcac agcctgcagg ataacctgcg cagccatgtg
      601 cacagcagcc gggaggcggc agcggccgga gatgtaacag acggaggcgg ctgtggctgt
      661 tattgagcaa aacgccaagc ctgtaaccga cgcgcttacg gcattaaggc ttgccgccat
      721 ggcattgccg ggtttgctaa tggcggcccc agccagtgca ggcgataaca tcagttttgt
      781 gcatgaacac tacgccgagg gcgagcggga tttgcagggg ctgagcagta aattccagcc
      841 aattgaggtg gattcgctgc tcggtgcggc gcactttagc cttaccgatg agatcagtgc
      901 cggccttaac ctcagccagg atacctggtc cggcgccacg cccatggcca ccgcccccag
      961 ctccgcccat ggcaaccgca tcgccccaca ctcacacacc cataccacca ccggcgcctc
     1021 catggaggaa ggcggccacc tgcacgatgc cgaaaacgac ccaaccccca gcgcgcccca
     1081 ggcgcaaacc ctcacgggtg cctcgcccta tttgtacagt gcgctaaaac tcgacagcaa
     1141 ccgcaggccg ctgcaaaccg atttggaggg taaggttatc ggcggggtgg ataaccagct
     1201 ggtgcatacc ctctccagcg cctcgccgga agtgcgccag cagtttgacc tgattcttcg
     1261 ccgccaggcg gcgcaatcca gcctgagcct ggggggcggg ctgtcccagg agcgcgattt
     1321 tggcgcctgg tttggccacg ccaagggcag ttgggatttc aaccgcaaac tcaccagcct
     1381 cggcgctggg attagctaca cccacagtaa aaccaacgcc ctgctggacc aggatgtggt
     1441 gccccatgta tatgagccct atatgctcac ctacgagcgg cgtggcgatg acagctacaa
     1501 ccgcagccac agcagcagcc gtttggaatt aggccaatac ggcccccggc ttaaagggga
     1561 caaagacgac tggggcctaa gcttgagcct gagccaaatc ctcaacccca gcgcctacct
     1621 gacagccgac ctgggcttta cccacagttt tggctaccag gccaatccct ataaagcagt
     1681 ggaggtgggc tttatcgacc cgctcaaaca aaaggggcag gcaggtggcg ttgcaacagc
     1741 taactatgtg tttgatgcgg agctggtggc actactggag cagcggccag acctgcgcaa
     1801 ccaggccagc gttaacctgc gttatgtgca gtacctaggc gcgtttgatg ccgcactgca
     1861 cctgggcctg cgcgcctata gcgacacctg gggggttaag gcgcagagcc tagatgcccg
     1921 gtgggtgcag cccctgggta aatggaccct caccccccgg ctgcgttatt acagccagtc
     1981 caaggccgat ttctatactc cctatttatt aacgcagcag ggcctgttcg cccaggtgac
     2041 agacccagtc aaaggcgcca tttacataga tacccgtaac accagcaacg gcctgcgcta
     2101 cttcgaagac cccaccggca ccgtcacccc ggccatagac accaaccccg cctcgcgcaa
     2161 atacggccag ccggtggtgg gcagccacgg ccgggctatc atcgagcagg gcagcggccg
     2221 ggcggtgaat gatcaaagcc tggtggataa cctcaaacaa gattccctgc cctttgaccg
     2281 cagcctattg ccgcaacact attccagcga tgcccgcctc tcggcctttg gcactctctc
     2341 agcaggccta atcgccagcc gcaaaatcaa taaggagcta agcgttgaat tcagctacga
     2401 acagctgcgc cacgcaggct ccctgaaatt cggcggcggt ggcgagggca gctatgccga
     2461 ttttgatgcc t
//
LOCUS       DAOQGS010000613         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27382_length_2461_cov_3.871987, whole genome shotgun sequence.
ACCESSION   DAOQGS010000613 DAOQGS010000000
VERSION     DAOQGS010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27382_length_2461_cov_3.871987"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..935
                     /locus_tag="PKZ52_16930"
     CDS             174..935
                     /locus_tag="PKZ52_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61510.1"
                     /translation="MSELPYCPKCQRPRSLPTKPMDACPHCGIIYAKYRAKHTDETLK
                     PLPEIEPTDEYRWPQKLINPWFYIPARVSIEVIIGRALVWVLFALWSIYFFRAGIDWE
                     KIGGSFLHNANLAFHEFGHVFFRPFGETLTILGGSLFQILLPFIITILFIYRQQEPFG
                     ASVTLWWCGQNWLDVAPYIDDAPDRALPLTTGNESGHDWGNLLTQWNALDQAHHLALQ
                     SRRIGMFIMALALAWGLWIVWQQYQNRTGEFVPDE"
     gene            1597..>2461
                     /locus_tag="PKZ52_16935"
     CDS             1597..>2461
                     /locus_tag="PKZ52_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046424.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNG61511.1"
                     /translation="MSNEPKCPFAAGRVKHTTASAPSNADWWPKQLKLSILHQHSPKA
                     NPLGEGFDYAAAFKQLDLDAVVQDLHALMTDSQDWWPADWGHYGGLMIRMAWHSAGTY
                     RTSDGRGGAGTGNQRFAPLNSWPDNGNLDKARRLLWPIKQKYGNKLSWADLMILAGNV
                     ALESMGFKTLGFAGGREDIWAPEEDIYWGSEATWLGDNRYSGERELEKPLAAVQMGLI
                     YVNPEGPNGNPDPVASGKDVRETFARMAMNDEETVALVAGGHTFGKAHGAGDPSLVGP
                     EPEGADIEEQGL"
BASE COUNT          637 a          672 c          608 g          544 t
ORIGIN      
        1 ccccctttcc aagggggagg ctgggtgggg gttttggcag aggcaaaata ccgtgaaaaa
       61 caggctaaaa tcctcggcct aaaaaccttg cattgttact gagcaattaa aaaataaaaa
      121 tacgccccaa aaaataacaa tctcattagc ctcttaagcc tggcccctaa accatgagcg
      181 aattgcccta ctgccccaaa tgccagcgcc cccgctccct gcccactaag cccatggacg
      241 cctgccccca ctgcggcatt atttacgcaa aataccgcgc taagcatact gacgaaacct
      301 taaaaccttt acccgaaatt gaaccaaccg atgaataccg ctggccgcaa aaattaatta
      361 acccttggtt ttatatccct gcgcgggtga gcatagaggt gattattggc cgcgccttgg
      421 tgtgggtttt atttgcgctc tggagtattt attttttccg cgcgggtatt gattgggaaa
      481 aaattggcgg ctcgtttttg cacaatgcca acctcgcctt ccacgaattt ggccatgtat
      541 ttttccgccc ttttggggaa accctcacca ttttaggcgg cagtctgttt caaatattat
      601 tgccgtttat cattactatt ttatttatct accggcaaca ggagcccttt ggcgccagtg
      661 ttaccctctg gtggtgtggg caaaattggc tggatgtggc gccttatatt gatgacgccc
      721 ccgaccgcgc cctgccctta accaccggca atgaaagcgg ccacgactgg ggcaatttat
      781 taacccagtg gaacgccctt gatcaagccc atcatctggc cctgcagagc cgccgtatag
      841 gcatgtttat tatggccttg gctttggcct ggggcttatg gattgtgtgg cagcaatatc
      901 aaaaccgtac cggtgaattt gtgccggatg aataaatagc ggcaattttt atatttgagc
      961 gggttaatgg attgcatggc tttgcattcc cacgcggtgg gcggaaacga aaagcgtgaa
     1021 aaccccctcc caacctcccc cttgataagg gggaggagca aaaaaacctg tctcgtatct
     1081 cgcccctcgc cccccacggg aatatacata aaacaccaaa taactaatta gtaggcctta
     1141 ttacccttcc acttgagcca cacattgaga ggaatttaac catgcgacag gatcgacgcg
     1201 gtgcagcagg ggtgaataca ggttattgca gaccgtcaaa aagagggacc tttttgacga
     1261 gcctacatgg atgtatttac ggcgtgtctg caataacctg tattcacccc tgcgggggca
     1321 gtatcaaagg ctcgcagcac taaagcactt gccggatggt ttctagccag cgcaagcatc
     1381 acgcccagtt acactaaaaa ggtactgaaa tagtgacaac cgaaaaaaac catacttaaa
     1441 ctgcggcaac taagcttttt cgcagaacct gccgcgcata tttgtcatca ctataagccg
     1501 cattggatta tttaattggg cacataccca taatcaacct agagttctgc taaccgcaac
     1561 aatcgccaaa actaaaaacc caacgaggaa attgccatgt cgaacgaacc taaatgcccc
     1621 tttgccgccg ggcgggttaa acacactacc gccagcgcac cttccaatgc cgattggtgg
     1681 cccaaacagt taaaactcag catccttcat caacactccc ccaaagcaaa tcccttgggc
     1741 gaaggcttcg attacgccgc cgcctttaaa caattagatt tagacgccgt ggtgcaggac
     1801 ctgcacgcac tgatgaccga ctcccaagac tggtggcccg ccgactgggg ccactacggc
     1861 ggtttgatga ttcgcatggc ctggcactcg gcgggcacct atcgcaccag tgatggccga
     1921 ggcggcgcgg gcaccggcaa tcagcgcttt gcgcccttaa atagctggcc ggataacggc
     1981 aatttggata aagcccgcag gctcttatgg cccatcaaac aaaaatacgg caataagctc
     2041 tcttgggcag atttaatgat cttggcgggc aatgtggccc tggaatccat gggctttaaa
     2101 accctcggct ttgccggtgg ccgcgaggat atatgggcgc cggaggaaga tatttactgg
     2161 ggctcagaag ccacctggct gggcgataac cgctacagcg gcgagcgaga attagaaaaa
     2221 cccttagccg ccgtgcaaat gggcttaatt tacgtaaacc ccgaaggccc caatggcaac
     2281 ccagacccag tagcctcagg caaagatgtg cgggagacct tcgcgcgcat ggccatgaac
     2341 gatgaagaaa ccgtggcgct agtagcgggc ggccacacct ttggcaaagc ccacggcgcc
     2401 ggggacccgt ccttagtggg gccggagccg gagggtgccg acatagaaga gcagggcctg
     2461 g
//
LOCUS       DAOQGS010000614         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27474_length_2455_cov_6.199583, whole genome shotgun sequence.
ACCESSION   DAOQGS010000614 DAOQGS010000000
VERSION     DAOQGS010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27474_length_2455_cov_6.199583"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKZ52_16940"
     CDS             <1..607
                     /locus_tag="PKZ52_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61512.1"
                     /translation="QNILNDALRVEIDTELSNIFKLQSIERHQPFTLSPPSAYTHFEN
                     GSIAATVFPLGVSLKLETGQILIKFDSNSDVHFKKQVGPHMTDSTMKLIVDSYLKSIE
                     DPQNEFVYLYEIREKLQRYFGTTKSMTTLLNIDKSDFGKFGKITCDEPIEQGRHRGKF
                     ENLRPATQEELFYCRGFIKNLIEKYAIYLTHKSDEQQITQN"
     gene            839..1339
                     /gene="lptE"
                     /locus_tag="PKZ52_16945"
     CDS             839..1339
                     /gene="lptE"
                     /locus_tag="PKZ52_16945"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNG61513.1"
                     /translation="MWRLLITLGLILGLSACGWQLRGVSSGHFPAQLQLASNDRYAPL
                     IASLGQAMSQRQVVSDDKAPLRLEVDPETLTKRTVAVTAIGSAAQYELTLSAPWRLYQ
                     RAGQTQTLLREGIAKSGRVFDFQPGNNLGKTEEETTLMAEMRREVANSILAQTQNLTS
                     QPASNP"
     gene            1347..2390
                     /gene="holA"
                     /locus_tag="PKZ52_16950"
     CDS             1347..2390
                     /gene="holA"
                     /locus_tag="PKZ52_16950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNG61514.1"
                     /translation="MAKINAEQLSRHLKPLAPVYCVSGDEPLLIQEAADAIRAAGRAQ
                     GFSERELFHVENHFDWGHFLQSANSLSLFASRKILEIRLNNKINDAGNKALVEYCNNP
                     SPDNLLLIISPKLDKSAQNAAWFKAVDKIGITCPIWPINEKQLPRWIEQRLHNAGLEV
                     DTDALALLAAKVEGNLLAAVQEIEKLKLIAEPGAINVHVMASAVADNARYDVFSLMDK
                     ALLNDSAAAVRHLNGLRGEGADPILILWGIAREVRTLLALKHAQHTGQPLDVAARNQG
                     IFEPHLSLLRQQLPRFKPAQLRLLLRQCALADRSMKGAAKLDTWGLLLDITLSLCGTR
                     VLNNGSLRALLKT"
BASE COUNT          691 a          599 c          607 g          558 t
ORIGIN      
        1 tcagaatatt ctgaatgacg ccttgcgagt ggaaatagat acagaattaa gcaatatttt
       61 taaacttcaa agcattgaaa gacatcagcc gtttacgctt agcccgcctt ccgcatatac
      121 gcattttgaa aacggatcaa ttgcggcaac tgtttttccg cttggagttt ctttaaaatt
      181 ggagactggt cagattttga taaaattcga cagcaattcg gatgttcatt tcaagaaaca
      241 agtcgggccg catatgacag attccacgat gaaactgatc gtcgatagct acttaaagtc
      301 aatcgaagat ccacaaaatg agtttgtata cctatatgaa ataagagaaa aattgcagcg
      361 ttattttgga acaacaaaat caatgacaac tctcttaaat atcgacaaat ctgatttcgg
      421 taaatttggt aaaattacgt gcgatgaacc aatagagcaa ggccgccatc gtggaaagtt
      481 tgaaaacctg cgccctgcca cccaagaaga gctgttttat tgcagaggat tcatcaagaa
      541 cttaattgaa aaatatgcaa tatatttaac ccataagtcc gacgagcagc agatcactca
      601 aaactaaatt aaactagcca gcaatggcgc cttgcaacag ccaacgaaat tgtacaaaga
      661 gggatgtggt tttacgcaac gaaacccgag gacaggatga actttctttg tgcttacttt
      721 tccttgtgca ctaataagaa aagccatttg ccattcctcg gtgccgaaat ccaaagggga
      781 gataaatcca accaagccgg gtagaatgcc cgctcattta atttagggtg cgttcagcat
      841 gtggcgattg ctaataacac tggggttaat cttgggcctt agcgcctgcg gttggcagtt
      901 gcggggggtg agcagcgggc attttccggc gcaattacag ctggcaagca acgaccgcta
      961 cgcgccctta attgccagcc tcggccaagc tatgagccag cggcaggtgg tgtcggatga
     1021 caaagcgcca ttgcgcctgg aggtggaccc ggaaacgctg accaaacgca ccgtggccgt
     1081 cacggccata ggctcggcgg cccagtacga attgaccctc agtgcacctt ggcgtttgta
     1141 tcagcgcgcc ggccagacgc aaaccctact gcgcgaaggc attgccaaaa gtggccgggt
     1201 gtttgacttc caaccgggca ataacctggg caaaaccgaa gaagaaacca ccctaatggc
     1261 ggaaatgcgc cgggaagtag ccaatagcat tctggcgcaa acgcaaaatt taacctccca
     1321 acccgcttcc aacccatagc acccccatgg ccaaaattaa cgccgaacaa ctcagccgcc
     1381 acttaaaacc cctcgcgccg gtttattgcg tgagcggcga tgaaccgcta ttaattcagg
     1441 aagccgccga tgccattcgc gccgctggcc gcgcccaggg ctttagtgag cgggaattat
     1501 tccatgtgga aaatcatttt gattgggggc attttttaca gagcgccaac agtttaagcc
     1561 tatttgccag ccgcaaaatt ttggaaattc gtttaaacaa caaaattaat gacgcgggca
     1621 acaaagccct agtggaatac tgcaacaacc caagcccaga taatttgcta ttaattatta
     1681 gccctaagct ggacaaaagc gcgcaaaacg ccgcttggtt taaagcggtg gataaaattg
     1741 gcatcacctg ccccatttgg cccattaatg aaaaacaatt gccgcgctgg attgagcagc
     1801 gcctgcacaa cgccggttta gaggtggaca ctgatgcctt ggccctgttg gcggccaagg
     1861 tggaaggcaa tttgttggcg gcggtgcagg aaattgaaaa actgaaatta attgccgaac
     1921 ctggggctat aaatgtgcac gtaatggcca gcgccgtggc cgataacgcc cgctacgatg
     1981 tatttagcct gatggataag gccctgttaa atgacagcgc agcggcggtg cggcatttaa
     2041 atggtttgcg cggtgagggc gccgacccaa ttttaatttt gtggggcata gcgcgggaag
     2101 tgcgcaccct attagcctta aaacacgccc aacacactgg ccagccccta gatgtggcgg
     2161 cgcgcaacca gggtattttt gagccccatt taagtttgct gcgccaacaa ttgccgcgtt
     2221 ttaaaccggc ccaattgcga ttgttgttgc gccaatgcgc tctggccgat cgcagtatga
     2281 aaggcgcggc caaactcgat acctggggat tattgctgga tattaccctc agcctctgcg
     2341 gcacgcgggt attaaataac ggcagtttgc gggcgctatt aaaaacctag cggctaacta
     2401 cctaaggaac ctctgaaaaa tgtaggcggc gaagcactag ctaggcgaat ttggc
//
LOCUS       DAOQGS010000615         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27532_length_2452_cov_4.934919, whole genome shotgun sequence.
ACCESSION   DAOQGS010000615 DAOQGS010000000
VERSION     DAOQGS010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27532_length_2452_cov_4.934919"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..>2452
                     /locus_tag="PKZ52_16955"
     CDS             331..>2452
                     /locus_tag="PKZ52_16955"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG61515.1"
                     /translation="MNLFARTFVLAVKNSRPVALCGQLNKSAASGLAISWFIASLACA
                     EETKRCAEFAARLDSLEGKVQWQVSGEKQWQSASLNQTFCYGDIILVQEKRAALRLSN
                     NTLVRLNEHSSIKLIPPQQSFWVELIDGAAHFLTRTPKSFTVKAPYVNAAVEGTEFLV
                     TAQNGVNQVGVIEGVVRSHNPAGSVLLTQGMSTQTGSAQQAPGAPVQLKLNDFVSWAM
                     YFPPLPNRLNLPVDIKTLLEQGQDGEVIARISKDAQASAEILSLGAALALNRGQLPQA
                     EELVQRAFKLNPKHPDAQAIAALLDLGRGEAERGQQRAAQNVLDNPFNPTALIAHSYS
                     QQGQGRLQGALDSALEARRIAPADSQIIARVAELYLALGQKAAARKALDEALALTPNH
                     SRLLTFKGFSLLAERKGKSAQTYFTKASQQDSADPYAQLGWGLSLIQQGKLETGREHL
                     ELAVILDPANSLLRSYLGKGYFEENRLKLAETQLNLAQTLDPNDPTPWYYLSQVKHDQ
                     HRYGEALQLVETAIDKNNNRAVYRADGAMGSDAAARLADKSRIYQAMGADQEAISAAS
                     DAIRTDPNDYAGHRAMAMALTGKEGNQTARANEALQASLLAPIGATPLAPGFAETAMQ
                     VLPGAGPHEMGINEYNAAFSPNGINGFVSALGAGNNTRATEGQVQLVGEKTDLSLGQY
                     HYYSDGFRENNDRDYLVNEARLKYQ"
BASE COUNT          591 a          701 c          663 g          497 t
ORIGIN      
        1 gtattaacct ggcccagaac gggttagaat cccaatttaa acaagcgctt gccgcttttg
       61 agcgcgcacc agcagaatcc ttacctctgt ggcaggcgct gacggcacaa tttccgcagg
      121 atggtcccag ccggtattac ctgcagcagt gccagcgagc gcttaatctc agcgcttaaa
      181 ttgtgtacgt gtaatggcgc gaaataatcg ttcgatgcag atgtgttggg cgctgcaaat
      241 taactcgaca atagaaaaat aaacctaatc tcgcaattct ttattcttcc agccggtgcg
      301 gctggattaa caactattag gaaattggtt atgaacctgt tcgcgcgtac cttcgtcctt
      361 gccgttaaaa atagccgccc agttgcactg tgcggccaac tcaacaagag tgcggcaagc
      421 ggcctggcga taagctggtt catcgcctcg ttggcctgtg cggaagaaac caaacgctgc
      481 gccgaatttg ccgcccgttt ggacagcctt gagggcaagg tgcagtggca ggtgagtggg
      541 gagaagcagt ggcagagcgc aagccttaac caaacctttt gttacggcga tattattctg
      601 gtacaggaaa aacgcgccgc attgcgttta agcaataaca ccctggtgcg tttaaacgaa
      661 cacagcagca ttaaattaat tcccccccaa caaagttttt gggtggaatt aatcgacggg
      721 gcggcacatt ttttaacccg tacgccaaaa agttttaccg ttaaagcccc ctacgtaaac
      781 gctgcggtag agggcaccga gtttttggtt accgcccaaa atggcgttaa ccaggttggc
      841 gtaattgagg gtgtggtgcg cagccacaac ccggcgggca gcgttttgct cacccagggc
      901 atgagcaccc aaaccggcag cgcccagcag gcacccggcg ccccggtgca attaaaactc
      961 aacgattttg tcagctgggc tatgtatttt ccgcccttgc ctaaccggct taacctgccc
     1021 gtcgatatta aaaccctgtt ggaacagggc caggatggtg aggtgatcgc gcgcataagt
     1081 aaggatgcgc aggccagtgc cgaaattctc tcgttaggtg ccgctctggc gttaaatcgc
     1141 ggccagttgc cgcaagcgga agaactcgtc cagcgcgcct ttaaactcaa ccccaaacac
     1201 ccggatgccc aggccatagc cgcattatta gatttaggcc gcggcgaagc cgagcgcggc
     1261 cagcagcggg cggcgcaaaa cgtattggat aatccgttta accccaccgc cttaatcgcc
     1321 cattcctaca gccaacaggg ccagggccgt ttgcagggcg ccttggattc cgccttggag
     1381 gcccgccgta ttgctccggc ggacagccaa attatcgccc gcgtagcgga gctgtatttg
     1441 gccctcggcc agaaggctgc ggcccgcaaa gcattggatg aagccctagc ccttaccccc
     1501 aaccacagcc gcctgctcac ctttaagggt tttagtttgt tggccgagcg caaaggcaaa
     1561 agcgctcaaa cctactttac caaggccagc caacaggatt ccgccgaccc ctatgcccaa
     1621 ttgggctggg gcctaagcct gatccagcag ggtaaattgg aaaccggccg tgagcatttg
     1681 gagctggccg ttatactcga cccggccaac agcctgctgc gctcttacct gggcaagggt
     1741 tattttgaag aaaaccgcct gaaacttgcc gaaacccaac tcaacctcgc ccaaaccctc
     1801 gaccccaacg accctacacc ttggtattac ttaagccagg tcaaacacga tcagcaccgc
     1861 tatggcgaag ccctgcaatt ggtggagaca gccattgata aaaacaacaa ccgggcggtg
     1921 taccgggcgg atggcgccat gggctcggat gccgccgcgc gtttggcgga taagtcgcgg
     1981 atttatcagg ccatgggggc ggatcaagag gccatatccg cagcctccga cgccatccgc
     2041 accgatccca acgattacgc cggccaccga gccatggcca tggccctcac aggcaaagag
     2101 ggcaaccaaa ccgcccgcgc caacgaggcc ctccaggcca gcctgctagc ccccatagga
     2161 gctacgccgc tagcccccgg ttttgccgaa accgctatgc aagtcctccc cggcgctggc
     2221 ccccatgaga tgggaattaa cgaatacaac gcagcgtttt cacccaatgg aattaatggg
     2281 tttgtatctg ctctgggcgc gggcaataac acccgcgcaa cggaagggca ggtgcagtta
     2341 gtgggggaaa aaacggattt gagtttgggg cagtatcatt attacagcga tgggtttagg
     2401 gagaataatg atcgggatta tttggtgaat gaggcgcggc ttaaatatca gg
//
LOCUS       DAOQGS010000616         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27552_length_2451_cov_4.535476, whole genome shotgun sequence.
ACCESSION   DAOQGS010000616 DAOQGS010000000
VERSION     DAOQGS010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27552_length_2451_cov_4.535476"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..935
                     /locus_tag="PKZ52_16960"
     CDS             195..935
                     /locus_tag="PKZ52_16960"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNG61516.1"
                     /translation="MHTSTRLTVPEHDSRIAVLVDCDNVTPDVLEYALRVIAQFGRVV
                     IRRGYGNHTTLANVWQKALVKLAFKPFLQYQYAAGKNTADIALALDALEAMFDNRADT
                     FCIVTSDSDFAHLCSKLRERGATVYIVGENKTPAALRNASDQFFECPQPEPVTEITTK
                     VVEPVKVVKRRPKSLIAAVALLTADTSEGRVGLGQLGQYLKRTDPGFSPKEYGHSGLL
                     SMVQTYPDLTITKDNNGGHSVSLTIKSE"
     gene            1002..1892
                     /locus_tag="PKZ52_16965"
     CDS             1002..1892
                     /locus_tag="PKZ52_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HNG61517.1"
                     /translation="MIGFVVVAIIAIYLVIKFKKPSGPASRRPTSKKQIKQGHPSQSA
                     VPGQSPRANKPQWEASYFKPPIKKGYQIFSHSLYVAGIEHRKDDAFTFYRSKSETLQL
                     QPEPENVHDKNAIKVLGIDDTHTYFLGYVPKKSAALLAKTKLTESVYPRLLKIAVNGD
                     HDININYTLVGPKSDKHKYDNYLNEQPASKEQKDYLIFFNIAVSKGLNSGQADALISE
                     HEKMASEFELDEWNNYQNILEYFKDDLRETEGIKKVSEALLIDVLKQLKQEGNSYERL
                     YEDIDEVVERLIEIKPELAC"
     gene            complement(1954..2181)
                     /locus_tag="PKZ52_16970"
     CDS             complement(1954..2181)
                     /locus_tag="PKZ52_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61518.1"
                     /translation="MNTPAAPVDTALNPVPDATITHIASHYFGIETLATRNRDALDFH
                     HIAVWNLKAALEAAYRAGHVSATGVNGGSGD"
BASE COUNT          720 a          592 c          567 g          572 t
ORIGIN      
        1 tcgatctatt aatatccgct ttttaaaccg caaagtagct gaatgcagtg catacccact
       61 cagttataca actgctatta tcggactagg ccaagcggtt gtttgaatgg agcgtcacag
      121 ataaaacggg caaacgaccc cgcccgccca agggttaact tctctaaaat ataaaattta
      181 atggaattga atttatgcac acgtcaacgc gattgaccgt tcccgaacat gacagccgaa
      241 tagcggtatt agttgattgt gataatgtga cgcccgatgt tttagaatac gcgttacgtg
      301 taatcgccca gtttgggcga gtagtcatac gccgtggcta cggcaatcac acgacgctag
      361 ccaatgtatg gcaaaaggcc ctagtcaagt tggcgtttaa gccttttctt caataccaat
      421 atgccgccgg aaaaaacact gctgatatag ccttagccct tgatgcatta gaggcgatgt
      481 ttgacaaccg cgcggacaca ttttgcatcg ttacaagtga ctccgatttt gctcatttat
      541 gtagcaagct cagggagcgc ggcgcgaccg tttacattgt cggcgagaac aaaacacccg
      601 cagcattacg aaatgccagc gatcaatttt ttgagtgccc ccagccggag ccggtaaccg
      661 aaataacgac caaggttgtc gaaccggtta aagttgtaaa acgtaggccc aaatcgctta
      721 tcgctgccgt tgcattattg acggctgata cctcagaagg tcgtgtagga ctcggccaat
      781 taggtcagta cttaaaacgc accgatccag ggttttcacc taaagaatat ggacactccg
      841 gactattaag tatggtacag acctaccccg atttaaccat tacaaaagac aataatggtg
      901 gccattcagt gagtttaaca attaagtctg agtaagttgg atatgtccag gcacagcgat
      961 gcactcaatc aataatccgc aaataatcta ggaaggtcct tatgataggg tttgtagtgg
     1021 tcgcaataat cgctatttat ttagtgataa aatttaaaaa accatctgga ccggccagtc
     1081 ggcgccccac atctaagaag caaattaagc aaggccaccc gtctcagtcc gccgttcctg
     1141 ggcaatctcc tcgagcgaac aaacctcaat gggaagccag ttactttaag ccgccgataa
     1201 aaaaaggcta tcaaatattt tctcacagtt tatatgttgc aggcattgag catcgaaagg
     1261 atgacgcatt tacattttat cgttcaaaaa gtgaaacgct ccagctgcaa cccgaaccgg
     1321 aaaatgttca cgataaaaat gcaattaaag tcttaggcat cgatgatacc catacctatt
     1381 tcttagggta cgtcccgaaa aagagtgcag cattactcgc caaaacgaag ctaacggaat
     1441 cggtctaccc acgattgctt aaaattgcgg ttaatggtga tcacgacatc aatattaatt
     1501 acaccttggt cggccccaag agcgacaaac acaaatacga taattatttg aacgaacagc
     1561 ctgcatctaa agagcaaaaa gattatttaa tcttctttaa catagctgtg agcaaagggc
     1621 tgaattccgg gcaagcagat gcgttaatta gtgaacatga aaaaatggct agcgagtttg
     1681 agctagacga gtggaataac tatcaaaaca ttctggaata ttttaaagat gaccttcgag
     1741 aaaccgaggg aatcaaaaaa gtgagtgagg cgctgctgat tgacgtcctg aagcaactga
     1801 agcaggaggg gaattcctac gagcgactgt atgaggatat tgacgaagtc gttgaacggt
     1861 taatcgaaat aaaacccgaa ctcgcctgct aattccgcgt ttccgacact agcgaggagc
     1921 caatactatt actcaggctt tccccacgac cgatcaatca ccacttccac cgttcacgcc
     1981 ggtggccgaa acgtgaccgg ctcgataggc cgcctcaagg gccgctttaa ggttccacac
     2041 cgcaatgtgg tgaaaatcca gcgcgtcgcg gttgcgcgta gccaaggtct caataccgaa
     2101 atagtggctg gcaatgtgag taatggtggc atctgggacg gggtttaaag cggtatcaac
     2161 gggggcggct ggggtgttca taagcattcc tcagggcgtg gtttgatgcc tccatgtacg
     2221 cgtcttcggc cctcaaaagc aagcattatg tagattaaat caataagtta ttaccataag
     2281 aggccaccgc tatcggcatc tccatccgcg cctatgcccg ccaccgaggc atcagcgata
     2341 ccgccgtgca caaggccatc cgcaccggcc gcattacccc tgaagccgac ggcaccctcg
     2401 accctgaacg cgcagatcgc gagtgggcac tcaataccga agcgctccgg t
//
LOCUS       DAOQGS010000617         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27569_length_2450_cov_4.286013, whole genome shotgun sequence.
ACCESSION   DAOQGS010000617 DAOQGS010000000
VERSION     DAOQGS010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27569_length_2450_cov_4.286013"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PKZ52_16975"
     CDS             complement(<1..1026)
                     /locus_tag="PKZ52_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61519.1"
                     /translation="MALRLSTFFVVAFYSATLFAQDPPAKTAPAAATTKPAPAAQLTL
                     PLEDLRLFAKAFENIRTGYVSEISDAKLLEYALKGMLSELDPHSAYLDSSSLEDLQTH
                     TTGEFGGLGIEVGGDENGVKVISPMDDRFLGDTPFSGPIPLGNHRLRVERRLFLTQLR
                     PLELAPGVAPLHFRVSLERSGLIDMALGGMIAGAGLGLMVLRLFLGEEIETLPRQEFY
                     KPLAAAALPAALGASEAGLAGWQMPAREAQLLIGSAGWGTIVGFGLGLGAQPQGPLPH
                     VLAVGFGVVGGTLGAAAYRFVQPSSGAIAMFNTTVLWSAVAGALGWAYVISERPETAF
                     YGHPSTGR"
     gene            complement(1047..2375)
                     /locus_tag="PKZ52_16980"
     CDS             complement(1047..2375)
                     /locus_tag="PKZ52_16980"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNG61520.1"
                     /translation="MKQLLSTAGIIACCLFGGMQQSFAADAANPETKLSELKNTIEQL
                     KKELISVKSNRENLSAELEASEKKISELNKKIEDLKAQLQNQQSSLNQLRGEREGLAS
                     AKKQQEAQVAQHLNAAYRLGNQSAIKLLLNQENPADLSRNLKYFEYLIAARNKQIQQF
                     AGTIKRLNELEPAITAQSEQLEANKQGVESQRQALNELIDERKRTLAKLNATIDDKDQ
                     ALKAYEQDRKGLQEVMAHIAQAEEKAKLQAEEKAKLKAKAAADKIELAEADEEADKNT
                     RKQNADNTAPKPAKLETGKTVSSSQTESAPLRGNLLQLKGELPWPTTGRLVNDFGTSR
                     VAGKVNWEGIYIQGTMGAPVKAIARGKVVFADYLKGHGLLIIIDHGQGYLSLYAHNQS
                     LLKKIGEPVEAGSVIASLGNTGGQADAGLYFELRHNGQPTNPKPWFKKSA"
BASE COUNT          459 a          658 c          703 g          630 t
ORIGIN      
        1 gcgccccgtc gaggggtggc cgtaaaacgc agtctcgggc ctctcgctga tgacgtaggc
       61 ccagcccagc gcgccggcca ccgcgctcca cagcacggtg gtgttgaaca tggcgatggc
      121 gcccgatgac ggctgcacga agcgataggc cgccgccccc agcgtgccgc cgaccacgcc
      181 gaacccgacg gccaggacgt gcggcagcgg gccctgcggc tgcgcgccga gccccagacc
      241 aaagccgacg atcgtgcccc agccggccga gccgatgaga agctgcgcct cacgcgctgg
      301 catctgccag ccggcgagcc ccgcctccga cgccccgagc gccgccggga gcgccgccgc
      361 cgccagcggc ttgtagaact cttgccgcgg cagggtctcg atctcttcgc cgagaaacag
      421 ccgcagcacc atcaggccca gccctgctcc ggcgatcatg ccgccgagcg ccatgtcgat
      481 aagcccgctg cgctccagcg agacgcggaa gtgcagcggc gcgacgccgg gggccagctc
      541 cagcggtcgc agctgggtca ggaacagccg ccgctcgacg cgcaggcgat gattgccaag
      601 aggtatcggg cccgaaaacg gcgtgtcgcc caggaagcga tcgtccatgg ggctgatgac
      661 ttttacaccg ttttcatcgc cgcccacttc aatacccaag ccgccaaatt cgccggtggt
      721 atgggtttgt aaatcctcta gagaggagct atctaagtat gccgagtggg ggtcaagttc
      781 gctgagcatg ccttttaagg cgtattctaa taatttcgcg tcgctaattt cgctgacata
      841 accggtgcga atattttcaa acgctttggc aaacaggcgt aaatcctcta ggggtagcgt
      901 cagttgcgca gccggggcgg gtttggttgt ggcagcagca ggggcagttt tggcaggtgg
      961 atcttgtgcg aaaagagtag ctgaataaaa agcgacaaca aaaaaagtgc taaggcgcag
     1021 cgccactgag attctccaaa attaatttag gcggattttt taaaccaggg tttggggttg
     1081 gtgggttggc cgttgtggcg caactcaaaa tataaaccgg catccgcttg gccgccggta
     1141 ttgccaaggg aagcaatgac actaccggct tccaccggtt cgccgatttt tttcaataag
     1201 ctttggttgt gcgcatatag gctgagataa ccctggccgt gatcaataat aattaataaa
     1261 ccgtggcctt ttaggtagtc ggcaaatacc actttgccgc gcgctatggc ttttacgggc
     1321 gctcccatag tgccttgaat ataaatgccc tcccagttta ctttgcctgc cactcgactg
     1381 gtgccaaaat cattaaccag gcggccagtg gtgggccagg gtaattcacc ttttaactgc
     1441 aacaaattgc cgcgcagtgg ggcactttcg gtttggctgc tggaaacggt tttgccggtt
     1501 tctaatttgg caggcttcgg tgcggtattg tcagcgttct gtttgcgcgt gtttttatcg
     1561 gcttcctcgt ctgcctcggc cagttctatt ttatcggccg ctgctttggc ttttagtttt
     1621 gctttttctt cggcctgcaa tttcgccttt tcttccgctt gggcgatatg agccattact
     1681 tcctgcaggc ctttgcgatc ctgttcatag gcctttaacg cctggtcttt atcatcgatg
     1741 gtggcgttga gtttggctag ggtgcgtttg cgctcgtcaa ttaattcatt gagggcctgg
     1801 cgttgggatt caacgccctg tttattggcc tctaattgtt cgctctgggc ggtgatggcg
     1861 ggctctaatt cgttgaggcg tttaatggtg cctgcaaact gctggatttg tttattgcgg
     1921 gcggcgatga ggtattcaaa gtattttaaa ttgcgcgata agtccgcagg gttttcctga
     1981 ttgaggagga gttttatcgc gctttgattg ccgaggcgat aggcagcatt aagatgctgt
     2041 gctacctgcg cctcttgttg cttttttgcg ctggcaaggc cctcccgttc accgcgtaat
     2101 tggttgaggc tggattgttg gttttgcagc tgagccttta aatcctcgat ttttttattc
     2161 agctcgctga tttttttctc cgaggcctcc agctccgcgc ttaggttttc acggttggat
     2221 ttaacggaaa tcagttcttt tttaagttgc tcgatggtgt tttttaattc gcttaacttg
     2281 gtctccggat tggccgcatc ggcagcaaaa ctctgctgca taccgccaaa caggcagcag
     2341 gcaataatgc ccgccgtgct taagagttgt ttcatcgtgt gtcctatggg ttattgagcc
     2401 actggttatt gtgcgactaa caggttgcgg ccggtcattt ccacaggctt
//
LOCUS       DAOQGS010000618         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27750_length_2440_cov_4.163103, whole genome shotgun sequence.
ACCESSION   DAOQGS010000618 DAOQGS010000000
VERSION     DAOQGS010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27750_length_2440_cov_4.163103"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..880)
                     /locus_tag="PKZ52_16985"
     CDS             complement(425..880)
                     /locus_tag="PKZ52_16985"
                     /inference="COORDINATES: protein motif:HMM:NF022426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HNG61521.1"
                     /translation="MKTYGCLFAMAIALSGCAGGVKEVACSGQNWAGFGYKMGSEGRS
                     VHEFDSHRSACGSNLEKGALKAYLDGYTRGIIEYCTYDNGYMVGSKNQSISDNCPAEL
                     RASYTKGYNLGKIEFAEKVRQMHNQVEDAESRQHKAQNENIMSSPRPGT"
     gene            complement(900..2276)
                     /locus_tag="PKZ52_16990"
     CDS             complement(900..2276)
                     /locus_tag="PKZ52_16990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG61522.1"
                     /translation="MRQIIKLNKCLMAWAIACVVSYAQASGAPFEIKVSKPEFVLPQF
                     TGPYQEREASIAPEEYERAEKLRSLLAEGRKAEVITELEKSYSIELSPAMLALQAQVY
                     FSLEMYEKAEDTYLTVLRRMPQLIRAHSDLGQLYLVREQPVKAREYFAKAVALGSHDA
                     AVYGQLGYLNLTLHGPFSAISSYQQAAAIEPDNMQWQQGLLAALTQAHMYEAAEALLD
                     GLIARQPAKVDFWLNKAALAMHKEDYRNAVIGLEMAIMLGDKDPRNLKSAAQLHLQLG
                     SFDRALELLDQSMVGNDLPMASIREYYYWLSQLGLWAKAETMLELAGKNMQRLKRDEQ
                     SQYYLLAAQLKAHQTQVAEAGRLFRQALELDPTNSEALLAFAGFAVKQKNYTDAELLY
                     VRAEAIPNSEKRAQLGRAQLYIDMQDYKAALNQLRAVYQKNPDAFEVKDNIEVIENII
                     RTNEAAAL"
BASE COUNT          627 a          636 c          524 g          653 t
ORIGIN      
        1 gtgcccctcg ccttgggcga gcgcgccgcc accgaatttc gccaccacgg ttttaatgtg
       61 cagtggcaaa catttaacat ggggcaccaa gtgaatttgg ctagtttagc ggcgctgggg
      121 gagtggttgg ggcgggtgtt aaccctttaa aaaccgacag cctagaacaa cagcgaggaa
      181 agaaagatac aaaatatcta tttaaggaga tccgcaaaag agcaattaaa aacctatagc
      241 agaataaaaa tactgatttt taaatttttg taacaatgca cgcaaccaca caaaagttca
      301 ggcgttgcac attataagcc tggcggtttt tcatatcggt ttaataatgg cggcaaggat
      361 gccagatcgg cggcaccagc cgccagatta agagccaaca aatcccaaat cccgatacaa
      421 ttctttaggt tccagggcgc ggagaactca tgatattttc attctgcgct ttgtgctggc
      481 ggctctcggc atcttcaacc tggttgtgca tctggcgcac tttttcggcg aattcgattt
      541 tgcccaaatt gtaccccttg gtgtagcttg cgcgcagctc cgccgggcag ttgtcactta
      601 tcgattgatt tttactgccc accatatagc cattatcgta ggtgcaatac tcgataatac
      661 cgcgggtata accatctaaa taggctttta aggcaccttt ctctaaattg ctgccacagg
      721 cggagcggtg gctgtcaaat tcatgcaccg agcgtccttc cgaacccatt ttataaccaa
      781 accctgccca attctggccg ctgcacgcaa cctctttaac gccgccggca cagcctgaca
      841 gggcaatagc catagcaaat aggcaaccat aggttttcat aaattactcc accaataaat
      901 taaagagccg ccgcctcatt agtgcgaata atattttcaa ttacttcaat attatccttc
      961 acttcaaacg catccggatt tttttgataa accgcccgca gctgatttaa cgccgcttta
     1021 taatcctgca tatctatata cagttgcgca cgccccaatt gggctctttt ttcgctattg
     1081 ggtattgcct cggcgcgcac atacagcaac tcagcatccg tataattttt ttgttttact
     1141 gcaaaccctg caaaagccaa cagtgcctca ctattggtgg ggtccaactc caaggcttgg
     1201 cgaaataacc gccctgcctc cgccacctgt gtctgatgag cttttaattg agcagccaat
     1261 aaataatatt ggctttgttc atcgcgtttt aaccgctgca tatttttgcc cgccaactcc
     1321 aacatagtct ctgccttggc ccacaaaccc agctggctca accaataata atattcgcgg
     1381 atactcgcca tgggtaagtc attgcctacc atgctttgat ccagcaactc taatgcgcga
     1441 tcaaaactgc ctaattgcaa atgtaactgc gccgccgatt ttaaattgcg cgggtcttta
     1501 tcgccgagca taattgccat ttcaaggccg ataacagcat tgcgataatc ctctttatgc
     1561 atagctaagg ccgctttatt taaccaaaaa tccactttgg ccggttgccg cgcaattaat
     1621 ccatccaata aagcctcagc tgcttcatac atatgtgcct gagttaaggc tgccaacaaa
     1681 ccctgctgcc attgcatatt atccggttct atagcggctg cttgctgata gctgctaata
     1741 gcactgaatg gtccgtgtaa ggttaggttt aggtagccca actggccgta cacggcggca
     1801 tcgtgggagc ctagggcaac tgctttagca aaatactccc gcgctttaac cggttgttcg
     1861 cgcaccaaat aaagctggcc taaatcgcta tgggcgcgaa tcagttgggg catgcgccgc
     1921 aataccgtta aataagtgtc ttcggccttt tcatacattt ctaaggaaaa atacacctgg
     1981 gcttgtaggg ccagcatggc ggggcttaat tcaatggaat aggatttttc caattcggta
     2041 attacctccg ctttgcgccc ttcggcgagt aagctgcgca atttctccgc ccgctcatat
     2101 tcttcagggg cgatagaggc ctcgcgctct tgataggggc cggtaaattg cggcaagaca
     2161 aattccggtt tgctcacttt aatctcaaac ggcgcgccac ttgcctgtgc ataactcact
     2221 acacaggcaa tagcccaggc cattaaacac ttatttagct ttatgatttg tcgcatgctt
     2281 agattcgatt aataaaagtt aagctataga gatacaccgc ctgcacgggc cggccattct
     2341 tggtaggcac cgtaaaacgc gagtcattga tggccttgcg aataacttcc accatttccg
     2401 ggtaaaccgg gtcaacgatt tttttcacat aagccttgcc
//
LOCUS       DAOQGS010000619         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27803_length_2438_cov_4.486781, whole genome shotgun sequence.
ACCESSION   DAOQGS010000619 DAOQGS010000000
VERSION     DAOQGS010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27803_length_2438_cov_4.486781"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /gene="gspK"
                     /locus_tag="PKZ52_16995"
     CDS             <1..1167
                     /gene="gspK"
                     /locus_tag="PKZ52_16995"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNG61523.1"
                     /translation="PMSRGGLGLSLQRQRGVVLILAMFIVVLVTSIAISTSWRFELDM
                     TRNENRWHGARIRNYGLSAENFVLKYAFDGDESGYDCLTPDMKDNRKLDFWAAEGMTW
                     DMNEASIDLKIIDLNRKFNLNMLVHKVQPKANQTFRTPADQYTEAQKMFIRLLQTIEI
                     DGQPMDTASAIEITDAVVDWLDADSEPTGSGGAEQNYYDSLEEPYPIGNRPMISVSEL
                     ALVKGVTPYLYSRLLPLVTAISPDSKLNINTMPQGLERIFNAKDVLEPFDARRLESIK
                     GNPRQQTNKNKQKNNGGEKADDNPALCSKSLDEAIEDSLILSDLAILPNALDKKALQE
                     FVDVKSEYFALYSTVKVGEITRVNKSLLHRSQSMGAGAQGSDPITVIRRTDGDF"
     gene            1302..>2438
                     /gene="gspL"
                     /locus_tag="PKZ52_17000"
     CDS             1302..>2438
                     /gene="gspL"
                     /locus_tag="PKZ52_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNG61524.1"
                     /translation="MSERIYVRKLEHNLWQWRHVTPQGQWASESFYTGDINLLAESVN
                     GKQVVLLLPGQNAVSMREETDLKDRKLISKVLPFQVEEHIIDSLDDLVFAYSPVKDGA
                     LTLCYVNEEYARASIDALESRGALVQRVLVDYLELAREDGSFTLILEHDQLMLADDQG
                     SGFCVELALAPLVLKSLFAEREPPRLVLVAEGEAELAELKALLPESVLALAAEASAEP
                     AEQDEADDDDLEAPLKPVNSRISEEIGGFWDYVLVQAKPAIDFRQGPLARKLPFAQWF
                     KEWRIPAAAAVIAFGLAMLAATLELTTSRKQYRQYVAERDGFYRQIIPGAGNVVDPVK
                     TLKAKLGGANQSPGSNLVGILNNVGPAISAHKDVKVSSFRYTHEN"
BASE COUNT          579 a          623 c          672 g          564 t
ORIGIN      
        1 ccaatgagta ggggcggttt ggggctgtca ctgcagcgac agcggggtgt ggtgctgatt
       61 ctggccatgt ttattgtggt gctggtgacc tctatcgcca tctccaccag ttggcgcttt
      121 gagttggata tgactcgcaa tgaaaaccgt tggcacggcg cgcgtattcg caattacggc
      181 ctgtccgcgg aaaactttgt tcttaaatat gcctttgatg gcgacgaatc cggatatgac
      241 tgtttaacac cggatatgaa agacaaccgc aaattggatt tttgggcggc cgaaggcatg
      301 acttgggata tgaatgaggc ctccatcgat ttaaaaataa tcgatttaaa ccgcaaattt
      361 aatctcaata tgctggtgca caaggttcag cccaaagcta accaaacctt tagaacaccg
      421 gccgaccaat acaccgaagc gcagaagatg tttattcgcc tgctgcaaac catcgagata
      481 gacggccagc ccatggatac cgcctctgcc attgagatca ccgatgcggt ggtggattgg
      541 ctcgatgcgg acagcgaacc tacgggtagc ggcggcgctg aacagaatta ttacgactcc
      601 ttagaagagc cctatcccat cggcaaccgg cctatgatca gtgtgtcgga gttggccctg
      661 gtgaagggcg taacccccta tttgtactct cgtctattgc cgttggttac ggcgatttcg
      721 cctgacagta aactcaacat caacaccatg ccgcaggggc ttgagcgcat atttaacgcc
      781 aaggatgtgt tagagccctt tgatgcccgc cgcttggaat ccattaaggg caacccgcgc
      841 caacagacca acaagaacaa acagaaaaat aatggtggcg aaaaagccga tgacaatccc
      901 gccttgtgtt ccaaaagctt ggacgaggct atagaggact ccttgatttt gtctgatttg
      961 gccatcctgc ccaacgccct ggacaaaaaa gccctgcaag aatttgttga tgtgaaatcg
     1021 gaatatttcg ccctctacag cacggttaaa gtgggcgaga tcacccgggt gaataagagc
     1081 ttgttgcacc gttcgcaaag tatgggggcg ggggctcaag gttccgatcc gatcacagtt
     1141 ataagaagaa ccgacgggga tttttgacaa ttattaggat cgaggcgata aactgctaaa
     1201 ttgccaacag ggcaggaatc cgcccactac aataatgaat aattgcgcag ctgcggctgt
     1261 gcgggaactg ggctgtggcc cgaagcctta agggtaatcg agtgtctgaa cgcatttacg
     1321 tccgcaaatt agagcataac ctctggcaat ggcgccatgt taccccgcag ggccagtggg
     1381 ccagcgagtc tttttatacc ggcgacatca atctgctggc cgagtcggtg aatggcaaac
     1441 aggtggtttt gctgttgccc ggccaaaacg ctgtttccat gcgggaggaa accgacctca
     1501 aagaccgcaa gctcatcagc aaggtgttac cctttcaggt cgaagaacac attatcgata
     1561 gccttgatga cctggtattt gcctatagcc cagttaaaga cggtgcgctc accctctgtt
     1621 acgtcaatga agaatacgcc cgcgcctcga ttgacgccct cgaatcccgg ggcgccttgg
     1681 tgcagcgcgt gctggtggat tatttggagc tggcccgcga ggatggtagc ttcacgctga
     1741 ttcttgaaca cgatcaattg atgctggcgg acgatcaagg cagcggcttt tgtgtggagc
     1801 tggccctggc gccgttggtg ctcaaatctc tcttcgccga acgagagccg ccccgtttag
     1861 tgctggtggc agaaggcgag gccgaacttg ccgaacttaa agcgctgttg ccggagtctg
     1921 ttttagcgct cgccgctgag gctagcgctg agcctgctga acaggacgag gcggacgatg
     1981 atgatttaga ggctcccttg aagcccgtta attcccgtat cagtgaggaa atcggcggtt
     2041 tttgggacta tgtattggtg caggccaagc ccgccatcga ttttcgccag gggcctttag
     2101 cgcgcaaatt accgtttgcg cagtggttta aggagtggcg cattcccgct gccgctgccg
     2161 tgatagcctt tggtttggcc atgttggccg ccaccctaga gctgactacc tcgcgcaaac
     2221 aatatcggca gtatgtcgcc gagcgcgatg gtttttaccg ccagatcatt cccggcgcgg
     2281 gcaatgtggt ggacccggtt aaaaccctca aggctaagtt gggcggcgcc aaccagtccc
     2341 ccggctccaa tttagtgggc attttaaata atgtaggccc agccatttct gcgcacaaag
     2401 atgtaaaagt cagcagcttt cgctacaccc acgagaat
//
LOCUS       DAOQGS010000620         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27916_length_2432_cov_4.919647, whole genome shotgun sequence.
ACCESSION   DAOQGS010000620 DAOQGS010000000
VERSION     DAOQGS010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27916_length_2432_cov_4.919647"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="PKZ52_17005"
     CDS             <1..1697
                     /locus_tag="PKZ52_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNG61525.1"
                     /translation="VLIYGYVRRLIAPLIQLSSVAHALGDGDYTAVMPKINVPEVEGL
                     ALALEIMRHKIIVRDKEILDNNARLQTSLDYADLIIESMPEAIIIVNANGTIIRANQQ
                     VKHLFGYSQEELIGNAVELLVPPQHRTQHSSHRTAYLASATSRPMGRNMELFAYRKDG
                     SKFPVEIGLNTLPGSGGLNVLATIIDLTHIKKNAEILLHANTRFSLAASAAGLGFWDY
                     DLASQTLQWDDTMYRIYGVENTPASAQPYSLWADAVHPDDLQPSEQALQASIQGPAPF
                     DTEFRIIHPNGAVRYIKAQAFVLRDATGAAQKLYGVNLDITERKKAEQHQQKLLREMT
                     AINAELNSFTYIASHDLKSPLRGIDQLASWIAEDLEGQLDDTTQKHLALMQNRIKRME
                     KLLDDLLEYSRAGRSTGDNSLVDSRALIDDIFDLIAVNKSARLKYLSPMPVFTTHKAP
                     LEVIFRNLIGNAIKHHPGGAIEIYIEAQKRKGGYEFGVGDNGAGIAPEHHQQIFTMFQ
                     TLKPRDVVEGSGIGLALVKKTVEGRGGTIRVESDGVSGSWFYFTWPDIHQPTPQEM"
     gene            1701..2135
                     /locus_tag="PKZ52_17010"
     CDS             1701..2135
                     /locus_tag="PKZ52_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG61526.1"
                     /translation="MLNTNLDRDVTILLVDDDDVDALSIQRTLKKQNLSNPLVRAHDG
                     VEGLELLRRLIKAGQRYIVLLDINMPRMNGFEMLSKVRADPTLASSLIFMLTTSQAED
                     DKAKSYQRNVAGYIVKKQSDNGFTRLIAMLHSYWGIVELPDC"
     gene            complement(2194..>2432)
                     /locus_tag="PKZ52_17015"
     CDS             complement(2194..>2432)
                     /locus_tag="PKZ52_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNG61527.1"
                     /translation="HYPAPGGKVKLAAAWLIEQAGCKGQAYQGITVHAQQALVLTNPE
                     RRPRTDVLACAAIIAKKVAEGFGIELEIEPQLLG"
BASE COUNT          637 a          620 c          599 g          576 t
ORIGIN      
        1 cggtactaat ttatggttat gtacgcaggc tgatagcacc cttaatacaa ttaagctccg
       61 ttgcccacgc cctaggtgat ggcgattaca ccgcggtaat gcccaagatt aatgtgccgg
      121 aggtggaagg gttggcctta gcactggaga ttatgcggca taaaataatt gtgcgggata
      181 aggaaattct cgacaacaat gcccgtttac aaacaagcct tgattacgct gatttaatta
      241 ttgagtccat gccagaggcg attattattg ttaatgccaa cggcactatt atccgcgcca
      301 accagcaggt gaaacacctg ttcggttatt cgcaggagga attaatcggc aatgccgtgg
      361 aattattagt gcccccgcaa caccgcacac agcattcatc ccaccgtact gcttatttgg
      421 ccagcgcaac ctcccggccg atgggcagaa acatggaatt gtttgcctat cgtaaagacg
      481 gcagtaaatt tcccgttgaa ataggcctta acaccttacc cggctcgggt ggcctgaatg
      541 tattagccac cattattgat ttaacccata ttaaaaaaaa tgcagagata ctgctgcatg
      601 ccaatacgcg ctttagttta gccgccagcg cagcaggcct tgggttttgg gattatgatt
      661 tggccagcca aaccctgcag tgggatgaca ccatgtaccg tatttacggc gttgaaaata
      721 caccagcatc ggcacagccc tattccctat gggccgatgc agtacacccg gacgatttac
      781 agcccagcga gcaggccctg caggcaagta ttcaaggccc cgcgccgttt gataccgaat
      841 ttcgcattat tcaccccaat ggcgcggtgc gttacatcaa agcccaggcg tttgtgctgc
      901 gcgatgccac aggcgccgcg caaaaactct acggcgttaa ccttgatatt accgagcgca
      961 aaaaagccga gcagcaccaa caaaaattat tgcgcgagat gacggctata aatgcggaat
     1021 taaatagctt tacctatatc gcctcccacg atttaaaatc gcccctgcgc ggtatcgacc
     1081 aattagccag ctggattgcc gaagatttag agggccaatt agatgacacc acgcaaaaac
     1141 atttagccct catgcaaaat cgtattaaac gcatggaaaa attgctggat gatctgctgg
     1201 agtattcacg cgctgggcgc agcactggcg ataacagcct ggtggatagc cgcgccctga
     1261 ttgacgacat ttttgattta attgccgtca ataaatcggc ccggcttaaa tacctatccc
     1321 ccatgcctgt gttcaccaca cataaagcgc cgcttgaggt gattttccgc aatttaattg
     1381 gcaacgccat aaaacatcac ccaggcgggg cgatagaaat ttatatcgaa gcgcaaaagc
     1441 gcaagggcgg ttatgaattc ggtgtcggcg acaacggcgc aggcatagcc cctgaacacc
     1501 accagcaaat atttactatg tttcaaaccc tcaagccccg cgatgtggtt gagggcagcg
     1561 gtattgggct ggccttagtt aagaagacgg tggaagggcg cggtggtact attcgcgttg
     1621 aatccgacgg agtatccggc agctggtttt atttcacttg gccggatatt catcaaccca
     1681 ctccccagga gatgtaagca atgttgaaca caaacctcga tcgcgatgtg acaattctgc
     1741 tggtagatga tgacgatgta gatgccctgt ccattcaacg cacgttaaaa aaacagaatt
     1801 tatccaaccc cttagtgcgc gcacacgacg gcgtggaggg tttggaacta ctgcggcggc
     1861 tgattaaagc ggggcagcgt tatatcgtgt tattagatat caatatgccg cgtatgaacg
     1921 gctttgaaat gctctccaag gtgcgcgccg acccaaccct ggccagctca ttaattttta
     1981 tgctgaccac ctcccaagcc gaggatgaca aggccaagtc atatcagcgc aatgtggcgg
     2041 gttacatagt taaaaaacaa tccgataacg gctttacacg cttaattgcc atgctgcatt
     2101 cctactgggg catagtggag ttgccggatt gttaaaagcg gcgaagagta attcccgaac
     2161 gcaatagttt gtgggctatt ttttgccgat cccttaaccg agcaattgcg gttcaatttc
     2221 caattcaata ccaaaaccct ctgccacttt tttagcaata atggccgcac aggcgagtac
     2281 gtctgtgcgc gggcgccgtt cggggttggt taataccaaa gcctgctggg cgtggacagt
     2341 gatgccctga taggcctgcc ctttacagcc agcctgctca attaaccagg cggcggccag
     2401 tttgaccttg cccccgggcg ctgggtaatg gg
//
LOCUS       DAOQGS010000621         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_27965_length_2430_cov_4.189053, whole genome shotgun sequence.
ACCESSION   DAOQGS010000621 DAOQGS010000000
VERSION     DAOQGS010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27965_length_2430_cov_4.189053"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1096)
                     /locus_tag="PKZ52_17020"
     CDS             complement(110..1096)
                     /locus_tag="PKZ52_17020"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG61528.1"
                     /translation="MSFSLAQAQRLKQQLGIFEAAKGQEASAEAVAYGRFYGLDQVGA
                     QHYAAGFYQPKGVLRKCGRLMTHYWRSPQAAKSHGTICLAPGLFDHAGLFAPLIKFCL
                     AEGFDVVCIEMPGHGLSDGQYCAIDSFFTYGAIWEAFILQQALNLTAPFIGLGQSTGC
                     TGLTALGLNPAGPLCGSIFFSPLVRPQHWLKVQTTYFALGNFLQKIARGLRINSHHEP
                     FNQLLLQDPLQPQFLSVQWVRALMAWVDALDDWPGAPSAQPVWIFQGSGDEVVDFEWN
                     LTCLTSRFARAHVHMIEGAKHHLANEAPEFTRTLWPAVASTLHKLRATTVEN"
     gene            1353..2249
                     /locus_tag="PKZ52_17025"
     CDS             1353..2249
                     /locus_tag="PKZ52_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNG61529.1"
                     /translation="MPRAWLLLLVLIAGCQNNTEEIAFGSTEYDLVRLTASANEVITA
                     IPVQEGQWVKAGTLLIQQDTQRAQAQLNLATAQLHEASAALEELTQGTRVQAIAAAAA
                     QVQEATARAHNAAAQAARGRSLRQANMLSQSDLDSLTTEQARAEAQVNAAQQTLDLLK
                     AGSRSEQLAQGRARVAAAAAAVELQRQRLAELSITAPSDGQVDALPYRVGERVAPGAL
                     LGTLLQGEQAYGRLYIPEQQRANLAIGDSLTLHISGLAQPVQGIVRSIAQEAAFTPYF
                     ALNQKERRPLGVFNRGKHPRWQ"
BASE COUNT          596 a          720 c          629 g          485 t
ORIGIN      
        1 cggcagggcc cgctccgatc actagaaagt cgtactgaaa atcaggcatg gaaaatccta
       61 tggagggaat gtgcgcggta ttattgcaaa aaccgacccc actttcctat taattctcca
      121 ccgttgtcgc ccgcagtttg tgcagggtgc ttgccaccgc aggccagagt gtgcgggtaa
      181 attccggcgc ttcgttggcc aaatgatgtt tggcgccctc gatcatgtgc acatgcgcgc
      241 gggcaaaacg gctggttaaa caggtgagat tccactcaaa atccaccact tcatcgccgc
      301 tgccttggaa tatccacaca ggctgggcac tcggtgcgcc cggccagtca tctaaagcgt
      361 ccacccacgc cattagggcg cgcacccact gcacggataa aaattgtggc tgcaagggat
      421 cctgcagcaa cagctgatta aacggctcgt gatgggaatt aatgcgcagg ccgcgggcaa
      481 ttttttgcag aaaattaccc agcgcaaaat aggtggtttg cacttttaac cagtgttgtg
      541 ggcgcaccag tgggctgaaa aaaatactgc cgcagagggg gccagccgga tttaatccta
      601 gcgccgtaag gccggtgcaa ccggtgcttt ggccaaggcc tataaaaggc gccgttaaat
      661 tcagcgcctg ctgcaaaata aaggcttccc aaatggcgcc ataggtgaaa aaactatcga
      721 tggcgcagta ttggccgtca cttaagccgt ggccgggcat ttcaatacac accacatcaa
      781 agccttcggc caggcaaaat ttaattaagg gcgcaaataa cccggcgtgg tcaaataaac
      841 ctggcgctaa acaaatagtg ccatggcttt tagccgcctg tgggctgcgc caataatggg
      901 tcatcagccg cccacatttg cgcagcacac ctttgggctg ataaaaaccc gccgcataat
      961 gctgggcgcc tacctgatcc agcccataaa agcgcccgta agcaaccgcc tcagcgctgg
     1021 cctcttggcc ctttgcggcc tcaaagatac ccaactgctg tttcagccgc tgggcctgcg
     1081 ccaaactaaa tgacatgata ttggctccgc ctaagtctta ctctgcagca tagcagttga
     1141 tatgggtatt taatgattac tcgatgcagc tattggcaat cgcctgcatt taataagtag
     1201 gcaaattacc cgaatatttt tttgacctca gccagatcat taaaccgcta caggattaaa
     1261 atccgcttac aatcaaacgc tgcctagccc ttattctcgc gggcttaaca agcgcgacac
     1321 tccccactta cctgtccatt aagaggttac ccatgccacg ggcttggttg ttattactcg
     1381 tgttaattgc cggctgccag aataatacgg aggaaatagc ctttggcagc acggaatacg
     1441 acctggtgcg cctcaccgct agcgccaatg aagtgattac ggctattcca gtgcaggaag
     1501 ggcaatgggt gaaggcaggc acgctactca ttcaacagga cacccagcgc gcccaggcgc
     1561 aattaaacct cgccaccgcg caattacacg aagccagcgc cgccctggag gaattaaccc
     1621 agggcacacg ggtgcaagcc attgccgccg ccgcagctca agtgcaggag gccactgccc
     1681 gcgcccacaa cgccgccgcg caggcggcgc ggggccgtag cttacggcag gccaatatgc
     1741 tcagccaaag cgatttagac tccctcacca ccgaacaggc gcgcgcagaa gcgcaggtaa
     1801 acgccgccca acagactctg gatttattaa aagcgggcag ccgcagcgag cagctcgccc
     1861 agggccgcgc ccgggtagcg gccgccgccg ctgcggttga attacagcgc cagcgcttgg
     1921 cggaattatc cataaccgcc cccagcgacg ggcaggttga cgccctgccc tatcgcgtcg
     1981 gcgagcgcgt cgcccctggc gccctactcg gcaccttatt gcagggcgaa caagcctatg
     2041 gtcgcttgta tatccctgag caacagcgcg ccaacctcgc cattggcgat tcactcaccc
     2101 tgcacatcag cggccttgcg caacccgtac agggcattgt gcgcagcatc gcccaggaag
     2161 ccgcttttac gccgtacttt gccttaaacc aaaaagagcg gcgcccgctt ggtgtattta
     2221 accgaggtaa gcatccccgc tggcaataaa aaaattcctg ccggtttgcc tgtgcaatgg
     2281 gtaaaaccat gaccaatagc gcagcaatgc cagcagccga taattacgcc atcgtcacgc
     2341 gcggcttaag ccggcagttc ggcggcacac gggcggtaaa taacttgaat ttagccattg
     2401 aaaaagcaca gatatttggc tttttaggcc
//
LOCUS       DAOQGS010000622         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28183_length_2419_cov_4.343063, whole genome shotgun sequence.
ACCESSION   DAOQGS010000622 DAOQGS010000000
VERSION     DAOQGS010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28183_length_2419_cov_4.343063"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="PKZ52_17030"
     CDS             complement(<1..1765)
                     /locus_tag="PKZ52_17030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNG61530.1"
                     /translation="MAASSQAGQLLAVKAEPEFDTLRLSLELSAPTQFRYSLLDKPDR
                     LVVELIATEASSSFNPATLKAPPVSSLRHSLNGKNLKLVFELDSSALVKAYKLRSGQQ
                     QPDRVVLELSGFKAVGVRKESSTDKAAAEEKQPSASKEPVVAKDPSARKEPSAQQEPS
                     AQKEPSAKKEPSAKKEPSAQKESSAPREPSALKDTPADKGSAAKEPVTKPANAEQAGA
                     DKPAAAKPGTKSSPRTLESAPVPQVVASKAESSSGQASPAQEVSVKENPRQESQPPIR
                     GRKIIVALDAGHGGKDTGAVGARGVREKDVVLAIARELANLLNQDGRYKVVMTRAGDQ
                     FIPLKGRRDKARKLKADVFISIHADAFVTAQAKGASVFALSQRGATSEMARFLAEHEN
                     SSDLLGSVGGVSLGDKDEVLAGVLVDLSMTATLANSLDLGGRVLKELGRMAELHNRSV
                     EQAGFVVLKSPDVPSILVETGFISNPEEAAALNSPAYRQKLAQAIYRGVNSYFATQGP
                     ALAQPASTEPSAPQPSPIPAASVRPDTPKIDPRADSRAQRLSPVQPKNSVQPNRAPAD
                     NRAGSPAPAKPTAQNHRVEKGD"
     gene            complement(1801..2277)
                     /gene="tsaE"
                     /locus_tag="PKZ52_17035"
     CDS             complement(1801..2277)
                     /gene="tsaE"
                     /locus_tag="PKZ52_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNG61531.1"
                     /translation="MPEVRLFLADEAATDRAGQSLAQALQTGGLVYLEGTLGAGKTHL
                     TRALLRALGHQGAVKSPTYTLVEPYELAALKVFHFDLYRLADPEELEFMGIRDYFAPG
                     HLCLVEWPSRGAGVLPAPDLTLQLAPQGQGRSLCASAGSSIGAAALQALAGISPSL"
BASE COUNT          418 a          732 c          765 g          504 t
ORIGIN      
        1 tatcgccttt ttccacccga tggttttgtg ctgtgggttt ggcgggggcc gggctgcccg
       61 ctcggttatc cgctggcgct cggttcggct gtactgagtt tttgggctgc acagggctaa
      121 gtcgctgcgc tcggctgtcg gcccgcgggt cgattttcgg ggtatcgggc cgcacgctgg
      181 cggcgggtat agggcttggt tggggagccg agggctctgt gcttgcgggc tgcgccaggg
      241 cggggccttg ggttgcaaaa taactgttca ccccgcgata aatggcctgg gcgagttttt
      301 gccggtaggc gggggagttg agagcggcgg cctcctccgg gttggagata aagcctgtct
      361 ccaccaggat cgacggcaca tccggggatt tgagcaccac aaaacccgcc tgctccacgc
      421 tgcggttgtg taattcggcc atgcggccca gttcttttaa aacgcggcca cccaagtcca
      481 ggctgttggc aagggtcgcc gtcatggata gatccaccaa tacaccggcc agcacctcat
      541 ctttgtcccc gaggcttacg ccacccacgg agccgagcag gtccgaactg ttttcgtgct
      601 cagccagaaa ccgcgccatt tcactggtag cgccgcgctg ggagagcgca aaaaccgagg
      661 cgcctttggc ctgagccgtc acgaaggcat cggcgtggat ggagataaat acatccgcct
      721 tcaacttgcg ggccttatcc cggcggcctt tgaggggaat aaactggtcc cccgcgcggg
      781 tcatcaccac cttgtagcgg ccgtcttgat tcaacaaatt ggccagctcc cgggctatgg
      841 ccagcaccac atctttctcc cgcacgccgc gcgcgcccac ggcgccggtg tctttgccgc
      901 cgtggccggc gtctagggcg acgataattt tgcggccgcg aatggggggc tggctctcct
      961 gcctggggtt ctccttgacg gaaacctcct gcgcggggct cgcctgcccg gaactggatt
     1021 cggctttaga cgcgacgact tgcggcactg gggcgctctc taaggtgcgc gggctcgatt
     1081 tagtgccggg cttggctgcg gcgggtttgt cggcgccggc ctgctcggca ttggccggtt
     1141 ttgtaacagg ctcttttgcg gcactgcctt tgtctgctgg agtgtctttt agcgctgagg
     1201 gctctctggg cgctgaagac tctttctgcg ctgagggctc ttttttggct gaaggctctt
     1261 ttttagcgga aggctccttc tgcgctgacg gctcttgttg agctgaaggc tcttttcgcg
     1321 ctgaagggtc cttggccaca acgggctctt tactcgccga gggttgtttt tcctctgcgg
     1381 ctgctttatc ggtgctgctc tctttgcgta cacccacggc cttaaagccg gataattcca
     1441 acactacccg gtccggttgc tgctggccag accgcagttt gtacgccttc accagggctg
     1501 agctgtccag ctcaaacacc agttttaagt ttttgccatt gaggctgtgg cgcaggctgc
     1561 tgaccggcgg cgccttcagg gtggcggggt taaagctgct gctggcctca gtggcaatca
     1621 gctccaccac caagcggtca ggtttgtcca acagggaata ccgaaactgg gtgggggccg
     1681 acagctccag ggacaggcgc agggtatcga actcaggttc agccttcacc gccagcaatt
     1741 ggcccgcctg ggaactagcg gccaaagccc accacagaat aagctgaatc gcaaaacgca
     1801 tcatagcgat ggggagattc cggctagggc ctgcaatgcc gctgcaccta tggagctgcc
     1861 agcactggcg cagaggctgc ggccctgccc ttggggggcc aactggaggg tgagatcagg
     1921 cgcgggcaaa acccccgccc cgcggctggg ccactccacc aggcacaagt gcccaggggc
     1981 gaaataatcg cggataccca taaactccag ctcttccggg tccgccaggc ggtacaaatc
     2041 gaaatgaaat accttgaggg ccgccaattc atagggctcc accagggtgt aggtggggct
     2101 tttaaccgcg ccttggtggc ctaatgcccg cagcagggcg cgggtcaaat gggttttgcc
     2161 cgcgccgagg gtgccctcca aatacaccaa gccccctgtc tgcagggcct gggccagact
     2221 ttggccagcg cggtcggtgg cggcttcatc agctaaaaac aaacgcactt cggggatgga
     2281 cacttactgg gcctcccaac cggcgcaaac ttggggaagc gcgccgatca aatcacccgc
     2341 gctgtggccg aaatgacaat tgcccgcgac caaagcatcc gccagggcgc cgtgcaagca
     2401 cacgcccagc agagcggca
//
LOCUS       DAOQGS010000623         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28236_length_2416_cov_5.160525, whole genome shotgun sequence.
ACCESSION   DAOQGS010000623 DAOQGS010000000
VERSION     DAOQGS010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28236_length_2416_cov_5.160525"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..795)
                     /locus_tag="PKZ52_17040"
     CDS             complement(55..795)
                     /locus_tag="PKZ52_17040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG61532.1"
                     /translation="MLFKPRIPADQPKLNWVESPWLRQPRMARWLMGSRTWYRQILKP
                     SCTAFAQLLKQPVPAQAAVLDAGCGEGLAFKLIERYFQPRIIFGLEIDKDCIANAISR
                     AGRLKTPTHVMQGDVALASFKDETFDIIFAHHLVQYSEDQTALVHKMVALLKPGGILM
                     SAEFCRVFTQSWRAKIIFRHPAATQKSAQEYVQLMTQAGLCVDDQECQYLRPWWARRL
                     WGLAQKLGFKGGEPAAVLFIARKRAAQP"
     gene            1247..2158
                     /gene="cysK"
                     /locus_tag="PKZ52_17045"
     CDS             1247..2158
                     /gene="cysK"
                     /locus_tag="PKZ52_17045"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNG61533.1"
                     /translation="MKANSILQTIGNTPHVRINHLFRPDIEVWMKLEHFNPGSSIKDR
                     IALAMIEDAEARGLINAQTVIIEPTSGNTGIGLAMVCAVKGYRLIIAMPESMSIERRK
                     YMQALGAELVLTPKEQGVSGAVAKANELLNEHPNSWMPQQFNNGANAEVHRRTTAQEI
                     LQDFPEGFDYLITGIGTGGHITGVSEVLKQHFPKMQTFAVEPAKSPVISGGEKGPHRL
                     QGLGPGFVPGVLNKSILDGILLVAEEDAFAKARLCAKKEGIFIGISSGATLAALEQKQ
                     TELPPGSKVLIFSYDTGERYLSIEDLF"
BASE COUNT          630 a          642 c          595 g          549 t
ORIGIN      
        1 gtgcagattc aagtattagg cggccgtagt agagttcaac tgcggttttt aggtttaagg
       61 ctgagctgct cgcttgcgcg caataaacag aacagcggcg ggctccccgc ccttaaaccc
      121 cagcttttgc gccagccccc acaggcgccg cgcccaccag ggccttaaat attggcactc
      181 ctgatcatcc acacacaaac cggcctgggt catcaactgc acatattcct gcgccgattt
      241 ttgtgtagcg gcaggatggc gaaatattat tttcgcgcgc caggattgag taaatacgcg
      301 gcaaaactcc gccgacatta aaatcccacc gggctttaag agggccacca ttttgtgcac
      361 taaagcggtt tgatcctcgc tgtactgcac aaggtgatgg gcgaaaataa tatcgaaggt
      421 ttcatcttta aaagacgcca aagccacatc cccttgcatt acatgggtgg gggtttttag
      481 ccgccccgcg cgggaaatcg cattggcaat acaatcctta tcgatctcca aaccgaagat
      541 aatcctcggc tgaaaatacc gctcaattaa tttaaacgcc aagccctccc cacacccggc
      601 atccaaaacc gccgcttgtg caggcaccgg ctgcttgagc agttgagcaa aggccgtaca
      661 actgggcttt aaaatctgcc gataccaagt gcgcgagccc atcaaccaac gcgccatacg
      721 cggctggcgc agccaagggg attccaccca attaagtttt ggctgatcgg cgggtattct
      781 cggtttaaac agcatgggta ggctcaaaaa agttctaaca aaagccagaa gcggggccag
      841 cacgctaacc tgcagggcca atttgagcaa gcgacgttca aacgaatatg gccagggagt
      901 gaactggttt gaccttgggt gcaaaacatt tcccaactga tttatgtaat ccccatgtac
      961 cagcgatctt tgccgagtta aggggtaata aatttgggcg cggttatatg taggccactt
     1021 cccgcccaga acgaataggt ccagccataa atgcgcaccg gcagaaagtt tgcgctgtga
     1081 tataagcagt tttatcgcta tacggccagg gcgatgagag taccgaaagc gctccaatta
     1141 ggcgtacact cccctcgccc agtgtgcagc gcaggcgctc aagctccaga ggcacacgca
     1201 tttttatgct taatttcctg actcaacccc aacacaggca gaccccatga aagccaactc
     1261 tattttgcag accatcggca atacccccca cgtgcgcatc aatcatttat ttcgcccgga
     1321 tattgaagtc tggatgaagc ttgagcactt taaccccggc agcagtatta aagaccgtat
     1381 tgcgctggca atgattgaag acgcggaagc gcgcggctta attaatgcgc aaacggtcat
     1441 tattgaaccc acctccggca ataccggcat tggccttgcg atggtgtgtg cggtgaaagg
     1501 ctaccggtta attattgcca tgcccgaatc catgtccatc gagcggcgca aatatatgca
     1561 agccttgggg gcagaattgg tgttaacacc taaagagcaa ggtgtaagcg gcgcggtggc
     1621 caaagccaat gaattattaa acgaacatcc taattcttgg atgccgcaac aatttaataa
     1681 cggcgccaat gcagaagtgc accgccgcac caccgcccaa gaaattctgc aggatttccc
     1741 agaaggtttt gattatttaa ttaccggtat tggcaccggc ggccatatca ccggtgtgag
     1801 cgaagtgctc aaacaacact ttccaaaaat gcaaaccttt gcggtggaac cggcgaaatc
     1861 cccggtcatc agcggcggcg aaaaaggccc ccaccgctta cagggcctag gcccaggttt
     1921 tgtgccgggc gtgttaaata aatctattct ggatgggatt ttactggtag cggaggaaga
     1981 tgcttttgct aaagcgcgcc tgtgcgcgaa aaaagaaggc atttttatcg gtatttcctc
     2041 aggcgccacc ttagcggcct tggagcaaaa acaaaccgaa ttgccgccgg gcagcaaggt
     2101 gttaattttt agttacgata ccggcgagcg gtatttatct attgaggatt tattttaatt
     2161 cagcttgctc ccactgatta attctcgttc ccacgcaaaa ctcgggaacg agaatctggg
     2221 atttctgata gaccccggat tgcgctgcgc tccatctagg ctacagaacc tatgtctacg
     2281 ggagattttg cccgcaacca ggaaccccca cccaacctcc cccttgcaag ggggaggagc
     2341 taaaaagcct taaatgaagt aattttgctt gttttcaagg cttttggctc cctcccctat
     2401 tcaaggggag ggctgg
//
LOCUS       DAOQGS010000624         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28250_length_2415_cov_4.236864, whole genome shotgun sequence.
ACCESSION   DAOQGS010000624 DAOQGS010000000
VERSION     DAOQGS010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28250_length_2415_cov_4.236864"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKZ52_17050"
     CDS             <1..330
                     /locus_tag="PKZ52_17050"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HNG61534.1"
                     /translation="VHTDPDAILRAGSVINELADTLFHNLYGSGANGKKIPRFERRAS
                     NNQVDPKHQKAFKAFLDKEGQAFLERVDDWLSAHETDDTTLPTAPKIRLGVGVYAIHK
                     SHDNHEA"
     gene            333..2207
                     /locus_tag="PKZ52_17055"
     CDS             333..2207
                     /locus_tag="PKZ52_17055"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNG61535.1"
                     /translation="MLPTSFRSGLGLLVFCALLSCGGGGGGGNAGIDGSGAPAIAVVS
                     GPINGFGSVIVNGVHYNTEHAEFWVRGVRATEAELNVGSYIHLEGTTSADGKEGVAKL
                     IYFQPNVIGAVSSVDLLAETFVVLGQTVHLTNDTALDFAFSPRDINGIRVGQQVEVSG
                     PLNAAGEVIATRVGLMQTATKELAGPVSDLDLAAKTFRINNITVSFAKASSLPELANG
                     QRVIVRGASVVNGTLQSNAIELEQKPQLPQGQLITQTGLITRFVSARDFTLGNFDVTT
                     SSATVYERAKASDLRLNARATIVGHLQDGKLVADKISLLADVEWKIQGPISDIRNMSL
                     EGGEILVQGSWLKLTAATRVDSELSMAGDRMKFANLRVDDFVVLTGHQEGSNQVVTSI
                     EREDRPQLDVEKKVLGFVQKTPLKDDADAFQLEPQLTVLLGESTQFYDNTAPISKEQF
                     VQKAAGKFVTVFGVMVDDGGFNTFKASSIQITASPATGGKGPKPGEKPSLPVQSPAVQ
                     SPVTTPVPVPPHKTEAAPVEGAKPAVPAPTKAPYTSKPERDSNGSEQTKYPKSPEVKP
                     ADQSTKGDGASTTAAEPAATDVLTNEPAEAAKPLAAPPQATEASSRPLPFHRPLTSKN
                     "
BASE COUNT          575 a          727 c          676 g          437 t
ORIGIN      
        1 gtacacaccg acccagacgc catactgcgg gcgggttctg tcattaatga gctggccgat
       61 acgctgtttc acaacctcta cggcagcggc gccaatggca aaaaaatccc gcgctttgag
      121 cgccgcgcca gcaataacca ggttgaccct aaacatcaaa aagcctttaa ggccttttta
      181 gataaagagg gccaggcctt cttagaacgc gtggatgatt ggttgagcgc ccacgaaacc
      241 gacgacacca cactcccaac agcacccaaa attcgcttgg gggtgggtgt gtatgccatt
      301 cacaaatcac acgacaatca cgaggcataa gcatgttgcc cacttcgttt cgctccggtc
      361 tggggctttt agtcttctgc gctttgctct cctgcggtgg tggcgggggc ggcggcaatg
      421 cgggcatcga cggctctggg gcaccggcca tcgcggtggt atccgggcct atcaatggct
      481 ttggcagcgt gatcgtcaac ggcgtgcact acaacaccga acatgctgag ttctgggtgc
      541 gcggcgtgcg cgctaccgag gccgaactga acgtgggcag ttatattcac ttagagggca
      601 ccaccagcgc cgatggcaaa gagggcgtgg ccaagctgat ttatttccaa cccaatgtga
      661 tcggcgcggt gagcagcgtg gacctgctgg cggagacctt tgtggttttg ggccaaaccg
      721 tgcacctcac caacgatacc gccctggatt ttgccttttc accccgggat atcaacggca
      781 ttcgcgtcgg ccaacaggtt gaggtatccg gccccttaaa tgccgccggg gaagtcatag
      841 ccacccgcgt aggcctgatg caaaccgcca ccaaagagtt ggcaggccct gtatccgatc
      901 tcgacctggc ggcgaagacc ttccgcatca ataacattac cgtgagcttt gccaaggcca
      961 gcagcctgcc tgaactggcc aacggccagc gggtaattgt gcgcggcgcc agtgtggtga
     1021 acggcacgct gcaatcgaat gccatcgagt tggagcaaaa accccagttg ccccagggcc
     1081 agctgatcac ccaaaccggc ctgattaccc gctttgtctc cgccagggat ttcaccctcg
     1141 gcaacttcga tgtaaccacc agcagcgcca ccgtgtatga gcgcgctaaa gcctctgacc
     1201 tgcgcttaaa tgcccgcgcc accattgtgg gccacctgca ggatggcaaa ttagtggccg
     1261 ataaaatcag cctgctggcc gatgtggagt ggaaaatcca aggccccatc agcgatatcc
     1321 gcaatatgag ccttgaaggc ggcgagatcc tggtgcaggg cagctggctg aaactcaccg
     1381 ccgccacccg ggtggacagc gagctgagca tggcggggga tcgcatgaaa ttcgccaacc
     1441 tgcgggtgga tgattttgtg gtactcaccg gccaccaaga gggcagcaac caggtggtca
     1501 ccagcattga gcgggaggat cgcccgcagc tggatgtgga gaaaaaagtg ctcggctttg
     1561 tgcaaaaaac cccacttaaa gacgatgccg acgccttcca attagagcca cagctgacgg
     1621 tactgctggg ggaaagcact cagttttacg acaacaccgc gccgatcagc aaagagcaat
     1681 ttgtgcagaa agcggcgggc aagtttgtca ccgtgttcgg ggtgatggtg gatgacggcg
     1741 gctttaatac ctttaaggcc agcagcattc aaattaccgc aagcccggca acgggcggca
     1801 aaggccctaa acccggtgag aaaccgtcac tgcctgtgca gagccctgcg gtgcaaagcc
     1861 ctgtgaccac gccggtgcct gtgccacccc acaaaaccga agcggcccca gtggagggcg
     1921 ccaaacccgc tgtacccgca cccactaagg cgccctatac aagcaaacct gagcgggata
     1981 gcaacggctc agagcagact aagtacccta aaagcccaga ggtaaaacct gctgaccaaa
     2041 gcaccaaggg cgacggcgcc agtacaactg cggctgaacc tgccgccacg gacgtcctaa
     2101 ccaatgagcc tgccgaagcg gcaaaacccc ttgcagcacc gcctcaagca acagaagcca
     2161 gcagccgccc cctgcctttt cacaggcctc tcacgagtaa aaattgaccc tagcggatag
     2221 cgtgatccgg gcctgagcaa acactcaggc tttttttgcc ggtggataaa ggccccaggc
     2281 ttttctaatt caacatgagc ggatgcgagc aaggccaaaa acccctcctt tgaaagcaca
     2341 ggcatgcagt taaaagttga acctataaac cgcagtgccc gatttcccct cccctggagg
     2401 ggtggcaggc tcgcc
//
LOCUS       DAOQGS010000625         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28270_length_2414_cov_4.914794, whole genome shotgun sequence.
ACCESSION   DAOQGS010000625 DAOQGS010000000
VERSION     DAOQGS010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28270_length_2414_cov_4.914794"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="PKZ52_17060"
     CDS             <1..1596
                     /locus_tag="PKZ52_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61536.1"
                     /translation="KTAIAEGLAKKIIDNEVPDILHGSVVYSLDLGSLLAGTKYRGDF
                     EKRFKGLLAELKKRKNAILFIDEIHTIIGAGAASGGVMDASNLLKPLLSSGELRCIGS
                     TTFQEYRGVFDKDRALSRRFQKIDVVEPTVDDTYKILKGLKSRFEQHFSLKYADGALR
                     AAAELSAKYITDRFLPDKAIDVIDEAGAHQLLLPASKRKKQITVKDIEDVVAKIARIP
                     AKTVSSSDKEQLAKLEDVLRMTVFGQPEAIDAVGSAIKLSRAGLTTGEKPIGSFLFAG
                     PTGVGKTELCRQLAKAMGVELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAV
                     TKHPHSVVLLDEIEKAHPDVFNLLLQVMDHGKLTDNNGRAADFRNVILIMTTNAGAEA
                     GGRASIGFTKQNHTTDALEVIKKTFSPEFRNRLDSIIQFGALSMDVIKTVVEKFLMEL
                     QSQLDHKKVTLDISDEAKQWLAVNGYDEKMGARPMARIIQEKIKKPLAEMVLFGSLST
                     AGGVVTIGVDVAKDTLVLNAEPA"
     gene            complement(1685..1987)
                     /locus_tag="PKZ52_17065"
     CDS             complement(1685..1987)
                     /locus_tag="PKZ52_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNG61537.1"
                     /translation="METVLQKAVALIYDGKGAPQVSAKGSGDDAQAIIELAQKAGVPL
                     CENAPLVDLLSRLELGDEIPQELYIAVAHIIAFAYQISQKAAPNVQEKTIMPGPQL"
     gene            complement(1987..>2414)
                     /locus_tag="PKZ52_17070"
     CDS             complement(1987..>2414)
                     /locus_tag="PKZ52_17070"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNG61538.1"
                     /translation="HSLAEQAKNPGTLMFEVPLPSAEGWITLSAQLTPLQHAQVDDDG
                     ASEKNARAPTATWRLFLEFNLPQNGLLAVQFLWQPGLLRGKVWANQPKLKHQLKDQLK
                     KLKQALQAGGINAEQLAFSDEPINKPAPPINQPLVDVQT"
BASE COUNT          597 a          515 c          619 g          683 t
ORIGIN      
        1 aaaaccgcca tagccgaagg cttagcaaag aaaattatcg acaatgaagt gcctgatatt
       61 ttgcacggca gcgtggtcta ctccctagat ttgggttctt tattagcggg caccaaatat
      121 cgcggcgact ttgaaaaacg ctttaaaggt ttgttggcgg agcttaaaaa acgcaaaaat
      181 gccattttat ttatcgatga aattcatacc atcatcggtg caggcgctgc ttccggcggt
      241 gtaatggatg cttctaattt attgaagcct ttattgtcgt ccggtgaatt gcgttgcata
      301 ggttctacaa cctttcaaga gtatcgcggt gtgtttgata aggaccgcgc gcttagtcgc
      361 cgttttcaaa aaatcgatgt ggttgagccc actgtggatg acacctataa gattttaaaa
      421 ggcctgaaaa gccgctttga acagcacttt agcctgaagt atgccgatgg tgccttgcgt
      481 gcggcagctg agttatcggc taaatatatt accgaccgct ttttgccgga caaagccata
      541 gatgttattg atgaggcggg cgcccatcaa ttattgctgc cagccagcaa gcgcaaaaaa
      601 caaatcactg ttaaagatat tgaagatgtg gttgccaaaa ttgcccgtat tcccgctaaa
      661 accgtctctt ctagcgataa ggagcagtta gccaagttag aagacgtgtt gcgcatgacg
      721 gtatttggtc agccagaagc cattgatgca gtaggctcgg cgattaaatt gtcccgcgca
      781 ggattaacca caggcgagaa acccataggt tcattcttat ttgcaggccc cacgggtgtg
      841 ggtaaaaccg aattatgtcg ccaactagcc aaggccatgg gggttgagct aattcgattt
      901 gatatgtctg aatatatgga gcgccatacc gtttcccgtc taattggtgc gcctcccggc
      961 tatgtgggtt ttgaccaagg tggcttgttg accgatgcgg tgactaaaca tccacactcg
     1021 gttgtattgt tggatgaaat tgaaaaagcc cacccagatg tatttaattt gctgctgcaa
     1081 gtgatggatc acggcaagtt aaccgataac aatggccgtg cggcggattt tcgcaatgtt
     1141 attttaatta tgaccaccaa cgcgggtgcg gaagctggcg gcagagcttc tattggcttt
     1201 accaaacaaa atcacaccac tgacgctttg gaagtgatta agaaaacttt ctcacccgag
     1261 tttagaaacc gtttggattc tattatccaa ttcggcgcgc tgagcatgga tgttattaaa
     1321 actgtggtgg agaaattcct gatggaattg caatcgcagc tggatcacaa aaaggttact
     1381 ctcgatatta gcgacgaggc gaaacagtgg ttggcggtga atggatatga tgaaaaaatg
     1441 ggtgctcgtc ccatggcgcg tattatccag gagaaaatca aaaaaccctt ggcagagatg
     1501 gtgttgtttg ggtccttatc taccgctggt ggcgtagtta ctattggcgt ggatgtggcg
     1561 aaggatactt tggttttgaa tgcagaaccg gcttaatatt taaatattgc cacaggtaaa
     1621 agcgagctaa ggctcgcttt ttttatgctt gaaacttttt tatcgactct atcgacggcc
     1681 gtagctacag ctgcgggccg ggcataatag ttttttcctg tacattaggt gcggcttttt
     1741 ggcttatttg gtaggcgaag gcaatgatat gtgccacggc aatataaagc tcttggggta
     1801 tttcatcgcc tagctctaac cttgataaca aatcgacgag cggtgcattt tcgcataggg
     1861 gcaccccggc cttttgtgcc aattcgataa tcgcctgtgc gtcatcgcca gagccttttg
     1921 cgctaacttg gggggcgcct ttgccgtcgt aaattaatgc cacggctttt tgcaatacag
     1981 tttccatcag gtttgtacgt cgactaaagg ttggttgatg ggcggggcag gtttgtttat
     2041 gggctcgtcg ctaaaggcta gctgctcggc atttatacca cctgcttgca aggcttgttt
     2101 taattttttt aactgatcct ttaattggtg tttgagttta ggttgattcg cccatacttt
     2161 gccgcgcagt agccccggtt gccacaaaaa ctgcacggcc aaaagtccat tttgcggtaa
     2221 attaaattcc aagaataaac gccaggttgc cgtcggtgca cgagcatttt tttcgcttgc
     2281 gccatcgtca tccacctgtg cgtgttggag gggggttaat tgggcggata aggttatcca
     2341 accctcagca cttggtagcg gcacttcaaa catcagggtg ccaggatttt tggcttgctc
     2401 ggccaggctg tgtg
//
LOCUS       DAOQGS010000626         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28350_length_2410_cov_4.503609, whole genome shotgun sequence.
ACCESSION   DAOQGS010000626 DAOQGS010000000
VERSION     DAOQGS010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28350_length_2410_cov_4.503609"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(783..1070)
                     /locus_tag="PKZ52_17075"
     CDS             complement(783..1070)
                     /locus_tag="PKZ52_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HNG61539.1"
                     /translation="MPSVNFHVLWPDGQSATYYSPSTIVHEHFTAGAHYSQEEFASRA
                     RAALDEASERVYKKFGYYCTAAMAEQEKIDGKLRQLREEQISGSVQLARFS"
     gene            complement(1057..2106)
                     /locus_tag="PKZ52_17080"
     CDS             complement(1057..2106)
                     /locus_tag="PKZ52_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HNG61540.1"
                     /translation="MNAQVSRSTSLAQDRQNTLDCLCEELRALPEIAAKTAIQLAAGL
                     AKPAQGLSLEQLYALPGDDCAAFKTADGYQLLAMEGMLPQFVQADPKAAGWSSVMVNI
                     SDIAAMGGRPTAVVNAFWHEDAAASGELLFHIKRACDVFGITFAGGHSSIRPGYSANL
                     AVAILGQAKNLLSCYHVKPGQRLFMLTDLTGSWHGNLPYWGCVQGKTPAQIRAQWNIP
                     AELAEADLAVAAKDISNGGILGTLIMMLELTGCGAIVDLSAIASPGGEMLRWLRAFQS
                     FGFLLAVEPDKVSALINYFQHSHLSCCPIGSFTDDGIIHLDYLGAAAQFWDVRQQPLT
                     AMGGTCAFGGNNALG"
     gene            complement(2103..>2410)
                     /locus_tag="PKZ52_17085"
     CDS             complement(2103..>2410)
                     /locus_tag="PKZ52_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG61541.1"
                     /translation="GAVRIYPVEDDFDALWFGGRLCVARAYRGHQAIGKSLINEAVSR
                     AIDLGCTRFLANVQPQNEAYFQSVHWQTLGAIEVAGRPHVRMQADLSAYPFMPRHPS"
BASE COUNT          593 a          662 c          601 g          554 t
ORIGIN      
        1 tgtaggctcg tcaaaaagat ccctcttttt gacggtctga aataacctgt attcaccact
       61 gctgcaccgc gaaccgatga tgccgcctgg ctagatcctc aaaaatttgt cgcctgcaca
      121 ggcaggagat gcactgtgtt gttacaaaaa aatggtttac tctcgttccc atgctctgcg
      181 tgggaacgcg tatttaatct tctgagcgaa cctgtacaaa ccaatctcta tgcattccca
      241 cgcggagcgt gggaacgaga cataaattac tctgccgtat cgatttttta ctgcgcatct
      301 tgcctgctgc cacatggaat gttggggttc gcgaagctca ccaccaacct acggttttaa
      361 tctcacccgc gctcgatgta ggatcgcatt tacaattatt actttacgct gccaaattaa
      421 tccctataca ttcccacgca gagcgtgaga acgagaaacg agcacagtgc gcttaaacca
      481 ttttgcatag gtcgctcgcg gtgtagctgc gataaataca ggttgtttca gaccgtcaaa
      541 aagagggacc tttttgacga gcccacaggg atgtgttcac ggcgtgtctg aaacaacctg
      601 tatttatcgc tgcgggcgag gactaaaggg ctagcccagc taaaagacac tcaacaatta
      661 caaaatattt aatttaaaat cgcctctaaa attcacccta tttgttctca cctgcgttcc
      721 cactcatagc gtgggaacga gatgcctttg cgcgatacct cacacacagc cgcataattt
      781 atttaactaa accgcgcaag ctgtaccgag ccgctaatct gctcttcgcg cagttgccgc
      841 aatttaccgt ctattttttc ctgctccgcc atagccgccg tacagtaata accaaatttt
      901 ttatacaccc gctcagacgc ctcatccagt gcagcgcgag cgcggctggc gaattcttcc
      961 tggctgtagt gcgcccctgc cgtgaaatgt tcgtgcacaa tggtcgaggg cgaataataa
     1021 gtagcgcttt gtccatcggg ccagagcacg tgaaaattaa ccgagggcat tatttcctcc
     1081 aaaagcgcag gtgccgccca tagcggtaag aggttgttgg cgcacatccc aaaattgcgc
     1141 ggcggcacct aagtaatcca agtgaataat gccgtcatcg gtaaaactgc caatcgggca
     1201 acaacttaaa tgggagtgtt gaaaataatt aattaacgcg gagactttgt ccggttctac
     1261 cgctaacaaa aaaccaaaac tttgaaaggc acgcagccag cgcagcattt cgccgcctgg
     1321 gctggcaatt gcgcttaaat caacgatggc gccgcagccg gttaattcca gcatcatgat
     1381 taaagtgcct aaaattccgc cgttgctaat atcttttgcc gccacggcta aatctgcttc
     1441 ggccaattcg gcaggaatat tccactgggc gcgaatttgc gcgggggttt taccctgcac
     1501 gcagccccaa tagggcaagt tgccatgcca actgccggtt aaatccgtca acataaataa
     1561 gcgctggccg ggttttacgt ggtaacagga cagcagattt ttcgcctggc ctaaaatcgc
     1621 caccgccaaa ttcgcgctgt agcctgggcg aatactgctg tgcccaccgg caaaggtaat
     1681 tccaaatacg tcgcaggcgc gctttatatg aaaaagtaat tcgccgctgg ctgctgcatc
     1741 ctcatgccaa aaggcattca ccactgcggt ggggcgcccg cccatggcgg ctatatcgct
     1801 gatattcacc atcaccgagg accaaccagc ggccttcgga tccgcctgca caaactgcgg
     1861 caacatgccc tccatcgcca gcagctggta gccatcggcg gttttaaagg ccgcgcagtc
     1921 atcgccgggc agcgcataaa gttgctcaag gcttagcccc tgtgcgggct ttgctaagcc
     1981 cgcggccagt tgaatggcgg ttttggcggc aatttccggc agggcacgca gctcttcaca
     2041 caggcaatcc agggtatttt ggcggtcttg agcgaggctg gtactacggg acacttgagc
     2101 gttcatgagg ggtgcctcgg cataaaggga taggcgctta aatcggcttg catgcgcacg
     2161 tgggggcggc cggccacttc aatggcaccc aaggtctgcc agtgaacgga ttgaaaatag
     2221 gcttcatttt gcggttgaac attggctaaa aagcgcgtgc agcccagatc aatagcgcgg
     2281 gaaacggctt cgttaatgag tgatttaccg atggcttgat ggccgcgata ggcgcgggcc
     2341 acacacaggc ggccgccgaa ccagagcgca tcaaagtcgt cctccaccgg gtagatgcgc
     2401 accgccccca
//
LOCUS       DAOQGS010000627         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28670_length_2394_cov_4.501924, whole genome shotgun sequence.
ACCESSION   DAOQGS010000627 DAOQGS010000000
VERSION     DAOQGS010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28670_length_2394_cov_4.501924"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /gene="rmuC"
                     /locus_tag="PKZ52_17090"
     CDS             complement(<1..1389)
                     /gene="rmuC"
                     /locus_tag="PKZ52_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNG61542.1"
                     /translation="MPELLVYSLSIIFLLASIVGLVWAMRLRTQLALAHQELLQTQQQ
                     ADSLRAEQAAVVHKYSALQAEQQHLVSRAAVLEEQISARQSQLTDANSQLVLLREKGD
                     KQAHQLTQLAQELAAAKAAEQERQAQQRLQQEQWHSQKVQLQQEFENLANKIFREKGE
                     QFARTSQEHLGLLLSPFREQISEFRQRVDAIHQETFAANSGLHVQIQQLQMLNQQITQ
                     DAQNLTLALKGDKKLSGSWGEMQLEKTLQLAGLVPGEHYSREVVLQHEDGASRRPDFI
                     IHLPDGKHLVLDSKVSLVDYDAACAASSEDDIKLALEAYVKALKRHIDGLAERDYSNL
                     VGVRSPSFVLMFLPLEAAYIEALKFNRELFNYGYNKNVILVAHTTLMPILRTVANVWQ
                     LDRSSKEAEAISARAGDIYNQACLVAERLLKLGKTLSTANAHYNETVRALAGNQGLHG
                     KLERFKDVAGNIT"
     gene            complement(1665..>2394)
                     /locus_tag="PKZ52_17095"
     CDS             complement(1665..>2394)
                     /locus_tag="PKZ52_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNG61543.1"
                     /translation="RLLFLETPSNPLNDVADLRALSQLAKHHNCLLAVDNCFCTPALQ
                     LPLALGADLVIHSATKYLDGQGRCLGGAVVGSHALVSEVLGFIRTCGPSMSAFNAWVF
                     SKGLETLRLRMDAHSANALELAQWLNEQDWVEQVFYAGLATHPGHQLAKAQQRGFGGV
                     LSFRVVGERAEAWAVLNACRIMSLTANLGDTKTTIVHPATTTHGRLSAEEKRAAGISE
                     NLIRIAVGLENSADLKADLLRYRR"
BASE COUNT          598 a          660 c          546 g          590 t
ORIGIN      
        1 ggtaatattg cccgccacat ctttaaaacg ctccaattta ccgtgcagcc cctgattacc
       61 cgccaaagcg cgcacggttt cattgtagtg agcattggcg gtgctgaggg ttttacctaa
      121 ttttaataag cgctcggcca ccaaacaggc ctggttgtaa atatccccgg cccgcgcgct
      181 gatggcttcg gcttctttgc tgctgcggtc aagctgccac acattcgcca cggtgcgcaa
      241 aatcggcata agggtggtgt gggccaccag aatgacattt ttgttatagc cgtaattaaa
      301 taattcccgg ttgaatttta gcgcctcaat ataggcggcc tccaaaggca aaaacatcag
      361 cacaaatgaa gggctgcgca cacccactaa attgctgtaa tcccgctcgg ccaagccgtc
      421 aatatggcgc ttaagcgctt taacataggc ttccagagcc aatttaatat cgtcctcgct
      481 gctggccgca caggccgcat cgtaatccac caaagagacc ttactgtcga gtactaaatg
      541 tttgccgtcc ggcaaatgaa taataaaatc cggcctgcgg ctagcgccgt cctcatgttg
      601 caacaccact tcgcggctgt agtgttcacc cggcactaaa ccggctaatt gcagcgtttt
      661 ctccaactgc atttcacccc aactgccact gagtttttta tcacctttta atgccagcgt
      721 taaattttgc gcatcctggg taatttgttg attgagcatt tgcaattgtt gaatttgcac
      781 atgcaacccg gaattagccg caaaagtctc ctgatgaatg gcatccaccc gctggcgaaa
      841 ctcgctaatt tgctcgcgaa atgggcttaa caacaggcct aaatgctctt ggctcgtgcg
      901 cgcaaattgc tcacctttct cgcggaaaat tttattggcc agattttcaa attcctgctg
      961 cagctggact ttttggctgt gccactgctc ctgttgcagc cgctgctgag cctgacgctc
     1021 ctgttccgcc gcttttgccg ccgctaactc ttgggccagc tgagtaagct gatgggcctg
     1081 tttatcacct ttctcgcgca acagcaccag ctgggaatta gcatcagtta attgcgactg
     1141 gcgggcgcta atttgctctt ccagtaccgc cgcgcgggac actaaatgtt gctgctccgc
     1201 ttgcaaggcg ctgtacttat gcactacagc cgcctgctcc gctcttaggc tgtcggcctg
     1261 ctgttgtgtt tgtagtaact cttggtgagc aagggctaat tgggtgcgca agcgcatggc
     1321 ccacaccaag ccgacaattg aggcgagtaa aaaaataata ctgaggctat aaacaagcag
     1381 ctctggcata aaaaccatat gcgattaaat ccctcgctgg gcaaaacgac tcgatttaga
     1441 tacagataaa tctggctaaa tacggccaaa ttccaaacat aaaaaagggg cttagctaat
     1501 aacgcccctt attaacccga cggcaaaata gatcgtcctg atctatttgc cgtcgctgca
     1561 ctaaacttac gaaaatgcgc ggattttctt gttttagtac tgctcgcggc ggttagcacc
     1621 gccgggttaa taaaactgcc cacaccagac ggtagaacaa taaactaacg gcgataacgc
     1681 aataaatccg cctttaaatc cgcactattt tctaacccca cagcaatacg aattaaattt
     1741 tcactaatcc ccgccgcacg tttttcttca gcgcttaaac gcccgtgggt agtggtggcc
     1801 gggtggacga tggtggtttt ggtatcacct aagttcgccg ttaaactcat aatgcggcag
     1861 gcatttaaca ccgcccaggc ttcagcccgc tcccccacta cacgaaacga taaaacgccg
     1921 ccaaatccac gctgctgcgc cttggctaat tgatggccgg gatgggttgc aaggcctgca
     1981 taaaatacct gctccaccca gtcttgctcg tttaaccact gcgccaattc cagcgcattg
     2041 gccgaatgag catccatgcg taaacgtagg gtttccaggc ctttgctaaa aacccaggca
     2101 ttaaatgcac tcatgctggg gccgcaggtg cgaataaaac ctaatacttc actcaccaat
     2161 gcatggctgc ccacaaccgc accgcccaaa cagcgcccct ggccgtctaa atattttgtt
     2221 gcactgtgaa taaccaagtc agcgcccaat gctaatggca attgcaaggc cggggtgcaa
     2281 aaacaattat caacggccag caggcaatta tggtgttttg ccaattggct taaggcgcgc
     2341 agatccgcca catcatttag cgggttggag ggggtttcca aaaataacag gcgt
//
LOCUS       DAOQGS010000628         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28688_length_2393_cov_4.281437, whole genome shotgun sequence.
ACCESSION   DAOQGS010000628 DAOQGS010000000
VERSION     DAOQGS010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28688_length_2393_cov_4.281437"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..>2393
                     /locus_tag="PKZ52_17100"
     CDS             67..>2393
                     /locus_tag="PKZ52_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61544.1"
                     /translation="MHLSLNGKAVEQPSLEAAGSLWLAEQAAGEEAQSDSLSLKVFRK
                     LTDGIPVVLTSRLELEVSGAEREVVLGPWLLAGFTPRAFSSDLPAKLEADGALRVQIK
                     PGQWTISLTSHSLSPKPALTLPPGSEDWPGQEVWSFEAAPHLRTVQVSGGASLDPSQT
                     TMPQEWRALPAYLLEPGQGLVLEELFRGNPNPAKDELRLARNLWLDFAGKELIFSDSL
                     TGTMKQSARLDLAQPFELLKADVNSRPQVVTQVVPGSAGVELREPNLRLEGQGRLAMA
                     GQIPVSAWGAEMSAVSWNLALPPGWSLLYARGADSASGSWVTLWGLFDIFMVLIIVIA
                     LGKILRPWVGALALLCLLITYQRSGAAVASWLNLAAVLALLPWVSGGFARWLKRYAWL
                     SFASLVLILLSFSIAQVRYALYPQLESSAGEGGFMESVFEDTFAMRKMEQAPMPMAEA
                     PAAPLASMAEAPGIFDGRLSEEEIVVTGVRASLPKKAGSQIRDYAMPSASQQIDPTQQ
                     TQTGSGVPQWNHNRVYLSWSGPVTADQTTHLYWVPPWINRPGYLLAAILPWLLAFALW
                     QGAGLLRIKLPSVNTGRALSLLLLAAVMGLGSAAPDTLADEASGKTPSLRGAEPSPEL
                     LKELKERLLKAPLCLPDCVSIEAVTLQVKEDSLRLELVLHALTPSIYILPASQTSWWP
                     QLALLNGERASLRQGDDELEVALPPGRQSLVLQGNLAGRNALPLAFGMNINNLKTDLT
                     GWRISGEPTAAAPSTTLQLSRAQGTSSHADKRLTPA"
BASE COUNT          454 a          747 c          731 g          461 t
ORIGIN      
        1 cttagcggcc gcttcagctg gagcagcttg ccccgcaccc tcagggtgcc agaggcgctg
       61 gcgattgtgc acttgagtct gaacggcaaa gccgtggagc agccgagcct tgaggcggcg
      121 ggcagcctgt ggcttgccga gcaggcagcg ggggaagaag cgcagagcga tagtttgagc
      181 ctgaaggtat tccgcaaatt aaccgatggc attccagtgg tgctcaccag ccgcctggaa
      241 cttgaagtga gcggcgccga gcgggaagtg gtgctcggcc cttggctgct ggcgggcttt
      301 accccccgcg ccttctcaag tgacctgccc gccaaactag aagccgacgg cgccctgcgg
      361 gtgcagatta aacccggcca gtggaccatc agccttacct cccacagcct cagtcccaaa
      421 cccgcgctta ctcttccccc aggcagtgaa gactggccgg gccaggaggt atggagtttt
      481 gaagctgcgc cccatttacg cactgtacaa gtcagcggcg gcgcgagcct agacccctca
      541 caaaccacca tgccccagga gtggcgcgcg ctgcccgcct atttgttgga gccggggcag
      601 ggcttggtgc tggaggagtt atttcgcggc aaccccaacc cggccaaaga cgagctgcgc
      661 ttggcgcgca acctctggct ggattttgcc ggcaaagagc tcatcttcag cgatagcctc
      721 accggcacca tgaaacaatc cgcccggctg gatttagccc agccctttga actgttaaag
      781 gccgatgtaa atagccgccc ccaagtggtt acccaggtag tgcccggcag tgccggggtg
      841 gagctgcggg agcccaacct gcgccttgag gggcaggggc ggctggccat ggccgggcaa
      901 attcctgtct cagcctgggg ggccgagatg tcggcggtca gctggaacct ggcactgccg
      961 ccgggctgga gcctgttgta cgcccgcggc gcagactcgg ccagcggctc ttgggtcacc
     1021 ctgtggggcc tgttcgatat ttttatggtg ctaattattg tgatcgccct gggcaaaatt
     1081 ctgcgcccct gggtgggggc cttggccttg ctgtgccttt taatcaccta ccagcgcagc
     1141 ggcgcggcgg tggccagttg gttaaacctg gcggcagtgc ttgctctgtt gccttgggta
     1201 tccggcggtt ttgcccgctg gctaaagcgc tacgcttggc tgagttttgc ctccctagtg
     1261 ctgattctgc tgagtttttc catagcccag gtacgttatg cactctatcc acaactggag
     1321 agttccgctg gggagggcgg gtttatggag tctgtgtttg aggatacttt tgccatgcgg
     1381 aaaatggaac aggcccctat gcccatggcc gaagcccctg ccgcgccttt ggcttccatg
     1441 gcagaagcgc caggcatatt tgacggtagg ttgagtgaag aggaaattgt ggtaaccggg
     1501 gtgcgtgctt cgctgccaaa gaaggctggc tcccagataa gagactacgc catgccaagc
     1561 gcatcccaac aaatcgaccc aacccaacaa acccaaaccg gctccggtgt accccaatgg
     1621 aatcacaatc gggtctacct tagttggagc ggcccggtca ctgccgatca aaccactcac
     1681 ctctactggg tgccgccctg gatcaaccgg ccaggttatc tgctcgcggc catcttgccc
     1741 tggctcttag ccttcgcttt gtggcaaggg gcaggcctgc tgcgcatcaa actgcccagc
     1801 gtaaacactg gccgcgccct gagcctcctg ttgttggccg ccgttatggg cctcggcagc
     1861 gcagcgccag acacactggc cgacgaggcc agcggcaaaa cgccgagctt acgaggtgct
     1921 gagcctagcc ccgagctgct caaggagcta aaagagcggc tgctcaaagc gcctttgtgc
     1981 ctgcccgatt gtgtgagcat cgaggcggtt actctgcaag tgaaagaaga tagcctgcgc
     2041 ctggagctgg tgctccacgc cctaacgccc agcatctata ttttgcccgc cagccaaacc
     2101 agttggtggc cgcaactggc cctgttaaat ggcgagcggg ccagcctgcg ccagggggat
     2161 gacgagctgg aagtggccct gccgccgggc cgccaaagcc tggtactgca gggcaacttg
     2221 gccggtcgca atgcgctgcc cctggccttt ggcatgaaca ttaataacct caaaaccgac
     2281 cttaccggtt ggcgcatcag cggtgaaccc acagcggctg cccccagcac cacgctgcag
     2341 ctcagccgcg cccaaggcac cagcagccat gccgataaac gcctcacgcc agc
//
LOCUS       DAOQGS010000629         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28761_length_2389_cov_4.249786, whole genome shotgun sequence.
ACCESSION   DAOQGS010000629 DAOQGS010000000
VERSION     DAOQGS010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28761_length_2389_cov_4.249786"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKZ52_17105"
     CDS             complement(<1..192)
                     /locus_tag="PKZ52_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNG61545.1"
                     /translation="MSTPIRRKNTSVIAWLQAQPSDFSFAQALRLLERASLLPQPSKN
                     SFVNLPIGRFSPPAREFARL"
     gene            complement(156..1988)
                     /gene="tssF"
                     /locus_tag="PKZ52_17110"
     CDS             complement(156..1988)
                     /gene="tssF"
                     /locus_tag="PKZ52_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HNG61546.1"
                     /translation="MADELLPYYEKELAFIRQLGNEFAKENPKIAGRLGINNETIEDP
                     HASRLIESFAYLNARIQHKLDDEFPELTDALINCIYPHYQRPIPALSIVQCEPDKAKL
                     DAPFLIPRHTLLETPQFNGETCRFRTVYDTTLLPIGVSHASLMGRPFTTPGAGQARGA
                     SSVLKLCLQGFTSDVNFGVLAPPRIRLYLKGQAQYTHPLYQLLLGECLQVVMTTSAQD
                     TQPIYLSPDVLKAVGFDQEDGLFPYPENSFIGYRLLTEFFTYAEKFMFVDIELGEKIS
                     PYYGQNLELYIYFKQADLELEHQLNADMFALNCTPIINLFPHKCDPIKLDHTQMEYPI
                     IPDARRPMGYELYAIQSVTAITTDGEASEFLPFYGLNHRHEGQTMQTFWFASRRHAKL
                     GNLDRDDGTDLYLSLVDLNFNPNAPDNRILSIESLASNRDQPSKLPFNSEQPALQCVN
                     AAPPCTRIRCLLQPTPAVRPPLGNKARWRLISHLNLNHLSLTGSSDSTEAFKEILRLY
                     DFRETSVTRALIDSVLQVKARPISAPLAIDGLTTLCRGVEIEVTLDAQLLSGSSSYLY
                     ATVLEHFFAVYCPLNSFTRTLVKIKNKEGYLKKCPPRSGEKILL"
     gene            complement(1981..>2389)
                     /gene="tssE"
                     /locus_tag="PKZ52_17115"
     CDS             complement(1981..>2389)
                     /gene="tssE"
                     /locus_tag="PKZ52_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNG61547.1"
                     /translation="PARHSKLKELRAAVRRDLENLLNTRYRVVAPAEHLELVNESFIN
                     YGLPDLATVNMIDGRKKKEFIKNIEFIIRTFEPRFKNVRVQFIDNEDKTDRTLRFRID
                     ALMYADPAPEEVIFDSILEPVLRTVSVEDSLHG"
BASE COUNT          559 a          500 c          719 g          611 t
ORIGIN      
        1 gaggcgggca aattcccgcg cgggggggct aaagcggccg atgggtaaat taacaaagct
       61 gtttttgctg ggttggggca aaagggatgc ccgctccaat aaacgcagcg cctgggcaaa
      121 actaaaatca ctcggctgcg cctgcagcca ggcgattaca gaagtatttt ttcgccggat
      181 cggggtggac attttttaag gtaaccttct ttatttttaa ttttcaccag ggtgcgggtg
      241 aaggaattga gcgggcaata gacggcaaaa aaatgttcca gcactgtggc atataaataa
      301 ctgctgctgc cgctgagtaa ttgggcatct aaggtgactt ctatttccac gccccggcac
      361 agggtggtga ggccatcaat ggcgagcggc gcgctaatgg ggcgggcttt tacctgtagt
      421 accgaatcaa ttaaggcgcg ggtaacggag gtttcgcgaa agtcgtacag gcgcagaatt
      481 tctttaaagg cttcggtgct atcactgctg ccggtgaggg ataaatgatt taaatttaaa
      541 tgggaaatta accgccagcg ggctttattg cctaagggcg ggcgcacagc gggggtgggt
      601 tgcagcaggc agcgaatgcg cgtgcagggg ggcgccgcat tcacgcattg cagggctggc
      661 tgctcggaat taaagggtaa tttgctgggt tgatcgcgat tactggccag gctttcgatg
      721 gataaaatgc ggttgtccgg cgcgttgggg ttaaaattta aatccaccag gcttaaatac
      781 aagtcggtgc catcgtctct gtctaaattg ccgagcttgg cgtggcggcg gctggcaaac
      841 caaaaggttt gcatggtttg gccttcgtgg cggtggttaa ggccgtaaaa gggcaaaaat
      901 tcgctggcct cgccatcggt agtaatggcg gtgaccgatt gaatggcata cagctcataa
      961 cccatgggcc tgcgggcatc ggggattatg gggtattcca tttgcgtgtg gtctaattta
     1021 attgggtcac acttatgggg gaataaatta ataatgggcg tgcaattaag cgcaaacata
     1081 tcggcgttta attggtgctc cagctctaag tccgcctgtt taaaataaat atacagctct
     1141 aggttctggc cgtaataggg gctgattttt tcgccaagtt caatatcgac aaacatgaat
     1201 ttttcggcat aggtgaaaaa ttctgtgagc aggcggtagc cgatgaagga gttttccggg
     1261 taggggaaga ggccgtcttc ctggtcaaaa cccacggctt ttaatacatc tgggcttaaa
     1321 taaatgggct gggtgtcctg ggcggaggtg gtcattacca cttgcaggca ttcacccaat
     1381 aataattgat agagcggatg ggtgtattga gcttggcctt ttaaatacag gcgaatgcgc
     1441 ggcggcgcca gcacaccaaa atttacatcc gaggtaaaac cctgcaggca gagttttaat
     1501 acgctggagg cgccgcgcgc ctggcctgcg cctggggtgg taaacgggcg gcccataagg
     1561 cttgcgtggc ttacgccaat gggcagcagg gtggtgtcat acacagtgcg aaagcggcag
     1621 gtttcgccgt taaattgcgg ggtttccagc agtgtgtggc gggggattaa aaacggcgca
     1681 tcaagtttgg ccttatccgg ctcacactgc actatggata acgcgggtat ggggcgctgg
     1741 tagtgggggt aaatacaatt aatgagtgcg tcggtaagct cgggaaattc atcgtcgagc
     1801 ttgtgctgaa tgcgggcatt taaataggca aaactttcaa ttaaacgcga ggcgtggggg
     1861 tcttcgatgg tttcgttatt aatgcctaag cgcccggcga ttttggggtt ttctttggcg
     1921 aattcattgc cgagctggcg gataaaggcc agttcttttt cgtaataggg taatagttca
     1981 tcagccatgt agcgaatctt ccacggatac ggtgcgtaat acgggttcta aaatcgagtc
     2041 gaaaataact tcttccggcg ctgggtctgc gtacatgagt gcgtcgatgc gaaagcgcag
     2101 ggtgcggtcg gttttgtctt cgttatcgat aaattgcacc cgcacgtttt taaaacgcgg
     2161 ctcaaaggtg cggataataa attcgatgtt tttaataaat tcttttttct tgcggccgtc
     2221 gatcatattc actgtggcta aatccggcag gccgtaattg ataaaacttt cgttgacgag
     2281 ttctaagtgc tcagctggcg ccacgacgcg gtagcgggtg ttgagtaagt tttctaaatc
     2341 ccgccgcacg gccgcgcgca attcttttaa tttgctgtgg cgggcgggg
//
LOCUS       DAOQGS010000630         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28896_length_2383_cov_5.422680, whole genome shotgun sequence.
ACCESSION   DAOQGS010000630 DAOQGS010000000
VERSION     DAOQGS010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28896_length_2383_cov_5.422680"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>2383
                     /locus_tag="PKZ52_17120"
     CDS             139..>2383
                     /locus_tag="PKZ52_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNG61548.1"
                     /translation="MNEYHTHNFQSVIDLEHHHIQASRERAGMPADLYTGFAISGGGI
                     RSASFALGVMQALVSANIFKKIDYMSTASGGGYIGSALTWFLRQGIKPDQPAGVEPDN
                     FPLGRARAPADAPQNLALNFIRMHGNYLTPTTKLNGWSLLGLVLRTSTISLVAYFTLL
                     STLMLVPMWLGIFKPITWLPQLRDTPLHIFLHNYFWLAALIAMAAFIVQCLWFAVFSG
                     IAWKSASLRYTVRTNAQKRLGTLLAIITLSFVIGSIPWLHQLSLELVLSLNMNDGYDA
                     WKLNASEEKANLAERLQRIQGAVAAISASIGSLMGFIQYVRARRGNTSDNSFIFYIAS
                     ALILYGLTYSAFVFNISIADFHTGRWIFFGGGLLAITLGLFVNTNYLGLHRMYRDRLI
                     EAFLPDHESAVRNQWQMAKGADTSMLDQFCQAPNERPFHLINTNLVLSDSHVSKYYSR
                     GGDSFVLSPLFSGSDATGWVKTWAWQRKPHNLNSPSSGMSLASAMAVSGASVNPNAGN
                     SGRGPTRSKLVSTLLTLLNIRLGYWVVNPKYVYQRWFKPNLIYPGLNGSLLGPGLHED
                     QAIVELSDGGHFDNLALYELIRRQVKLIILSDAGADPTYCFDDLGNAVEKVRVEFGVN
                     IRFSDEYSLDNVQPGSAPKSCNTAFDVARHSFATAAIEYPDGSKGRLIYIKATLIEGL
                     PADVYNYRKSNPAFPHEPTADQFFSEVQIEAYRELGYRLTWDMLNKNGIIGADGKWQP
                     ERHLDMLK"
BASE COUNT          589 a          597 c          536 g          661 t
ORIGIN      
        1 tgagggactc ctgtctgcag cagttttgca aataacaccc gatcaagttt tgcaaatacc
       61 acccgatcag aggttcctac aaaaatcgcc acacctatat tgggttcact actattgggc
      121 ttactacgga attaaagtat gaatgaatac catacccata attttcaaag cgttattgac
      181 ctagagcacc atcatataca ggcaagccgc gagcgcgccg gaatgcccgc tgacttatat
      241 actgggtttg ccatttccgg tggtggaatt cgatctgcat cttttgcgct aggtgtaatg
      301 caggcattgg tatcggccaa tatattcaaa aaaatcgatt atatgtccac cgcttcaggc
      361 ggcggctata taggttcggc tttaacctgg tttttacggc aaggtattaa acccgatcaa
      421 ccagcaggag tggagcctga taattttccc cttggccggg cgcgtgcgcc cgcagatgcc
      481 ccgcaaaact tggcgctaaa ttttatccgt atgcacggca attatttaac gccaaccacc
      541 aagctcaatg gttggtcatt attaggtttg gttttgcgca ccagcactat ttcactggtg
      601 gcctatttca cgctgctctc cacactaatg ctggtaccca tgtggctggg tatttttaaa
      661 cccattacct ggctgccgca attgcgtgat acccccctgc atatatttct gcacaactat
      721 ttttggctcg ccgcacttat tgccatggca gcatttattg tgcagtgttt gtggttcgcg
      781 gtgttttcag gcatagcctg gaaatcggcc agtttacgct acaccgtccg caccaatgca
      841 caaaagcggc tgggcacatt gttagccatt attacattaa gttttgtgat tggctccatt
      901 ccctggctgc accagttgag cctggaattg gtgctgtccc tcaatatgaa tgacggctac
      961 gacgcctgga aactgaatgc atcggaagaa aaagccaatt tagctgagcg cttgcaacgc
     1021 atacaaggag ctgtagcagc catttctgca tccatcggtt cactgatggg atttattcaa
     1081 tatgtacgcg ctcgccgcgg caacaccagc gataactcat tcatttttta tattgcctcc
     1141 gcactcatcc tctacggtct tacctactct gcgtttgttt ttaatatttc cattgccgat
     1201 ttccacactg gccgctggat attttttggc ggcggtttac tggccattac cctggggctg
     1261 tttgttaata ctaattatct tggcctgcac cgcatgtatc gcgatcgttt aatcgaggcg
     1321 tttttgcctg atcacgaatc cgctgtgcgc aaccagtggc aaatggcaaa aggcgctgat
     1381 acttctatgc tcgatcaatt ctgccaagca cccaatgagc gcccctttca tctgatcaac
     1441 accaatttgg tattaagcga ttcacacgta tcaaaatatt actcgcgcgg tggcgacagc
     1501 tttgttttat ccccactatt ttctggctca gatgccaccg gctgggtaaa aacctgggct
     1561 tggcagcgca aaccccacaa cttaaactcg cctagctccg gcatgagcct cgcctccgct
     1621 atggcggtat cgggcgcatc ggttaacccc aatgccggca attctgggcg cgggccaact
     1681 cgctcaaagt tagtgtcaac acttttaacc ctgctaaata ttcggctggg atattgggtg
     1741 gtaaatccca aatacgtcta ccagcgctgg tttaaaccga atttaattta tccgggttta
     1801 aatggcagtt tattaggccc gggtcttcac gaggatcaag ccatcgttga gttgagtgac
     1861 ggtgggcatt ttgacaattt agcactctat gagttaattc gcaggcaggt aaaactcatt
     1921 atactttctg atgctggtgc cgaccctacc tattgttttg atgacctggg caatgcggta
     1981 gaaaaagtac gggttgagtt cggcgtaaac attcgtttta gcgatgaata tagcctggat
     2041 aatgtacaac ctggctccgc ccctaaaagc tgcaataccg catttgatgt tgccagacac
     2101 agctttgcca cggcggctat tgaataccct gacggcagta aaggccgttt aatttatata
     2161 aaagccacct taattgaagg cctacctgcg gatgtgtaca attacagaaa atcaaaccct
     2221 gcgtttcctc acgaacccac tgcagatcaa ttttttagcg aagtacaaat tgaggcctat
     2281 cgagaattag gataccgttt gacgtgggat atgctcaaca aaaatggcat catcggcgcc
     2341 gatggcaaat ggcaaccgga acggcactta gatatgctaa aac
//
LOCUS       DAOQGS010000631         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_28988_length_2378_cov_5.059406, whole genome shotgun sequence.
ACCESSION   DAOQGS010000631 DAOQGS010000000
VERSION     DAOQGS010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28988_length_2378_cov_5.059406"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKZ52_17125"
     CDS             <1..195
                     /locus_tag="PKZ52_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG61549.1"
                     /translation="APVGSIGLIYVPALFVLTPISSVMAPVGVWLNKRLPEAWLVRLF
                     AVFLLLVAGRMGYLAFEGIV"
     gene            complement(241..1176)
                     /gene="tal"
                     /locus_tag="PKZ52_17130"
     CDS             complement(241..1176)
                     /gene="tal"
                     /locus_tag="PKZ52_17130"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNG61550.1"
                     /translation="MTNKLVQLKQFSDVVADTGDIEAIARYQPLDATTNPSLLFKAAQ
                     MPQYQDLVKQTLASTASIGDAADKIGVAIGCEILKIVPGRVSTEVDARLSFDTEASIA
                     KAHKLIDLYAAQGISKERVLIKLASTWEGIRAARTLEKEGINCNLTLLFSFAQAAACA
                     DAGAFLISPFVGRILDWYKANTAKKEYAAAEDPGVISVTNIYNYYKEHGYKTVVMGAS
                     FRNIGEIEALAGCDRLTISPELLAALETDTGTLSRALEPGKVKTGGAKVVETEAQFRF
                     NMNEDQMATEKLSDGIRKFVADQINLENFLASVKG"
     gene            complement(1202..2227)
                     /gene="dusA"
                     /locus_tag="PKZ52_17135"
     CDS             complement(1202..2227)
                     /gene="dusA"
                     /locus_tag="PKZ52_17135"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNG61551.1"
                     /translation="MHSTDTSFNPWRFCTAPMMEWSDAHTRYFWRLMSRHTRLYTEMV
                     TTGALIHGNRARFLHYHPAELPLALQLGGHSAQDLSLCARMAEDAGFSEVNLNCGCPS
                     DRVQEGRIGACLMAEPELVADAVHRMISTTSIPITIKHRLGIDELDADEHLHRFVALN
                     TQAGCQVFIVHARKAWLKGLSPKENREIPPLQYERVVALKKAFPALTFVVNGGITTLE
                     QCATHLEQLDGVMLGREAYYNPYLLAQVDGTLFNDEAPIAARGEILARLEPYIEAHLA
                     AGGRLHAITRHLLGLFSAQPGGRLFRRHLSEEANKPTAGLDVLRAALAFTQPTPTGGC
                     HSLAQHP"
BASE COUNT          521 a          587 c          665 g          605 t
ORIGIN      
        1 gctcctgtag gctctatcgg gttaatttac gtgcccgctt tatttgtgct tacaccgatt
       61 agcagtgtca tggcgcctgt tggcgtatgg ctgaataaac gcttgccgga ggcctggcta
      121 gtgcgcttat ttgcggtttt tttgctgctg gtggcgggca ggatgggcta tttggcgttt
      181 gagggtatcg tctaaaaata acagcgcttc cctgcgcctt ctctttatcc ttaaagagaa
      241 ttaaccctta acagaggcta agaaattttc caggttaatt tggtcggcca caaacttgcg
      301 aataccgtcg ctgagttttt ccgtagccat ttgatcttca ttcatgttaa agcgaaattg
      361 cgcttcagtt tccaccactt tggcgccgcc ggttttaacc ttgcccggct ctaaagcgcg
      421 gcttagggtg ccggtgtcgg tttccagggc ggctaataat tcagggctaa tggttaaacg
      481 atcgcacccg gccagagctt caatctcacc tatattgcga aagcttgcgc ccatcactac
      541 ggttttatag ccgtgttctt tgtagtagtt gtaaatgttg gtgacggaaa tgacacctgg
      601 atcttcggct gcggcgtatt cctttttggc ggtattagct ttgtaccagt caagaatacg
      661 gcccacaaat ggcgaaatta aaaacgcacc ggcatcggcg caggcggcgg cctgggcgaa
      721 gctaaataac agggttaaat tgcaattgat gccttctttt tccagggtgc gggcggcgcg
      781 aataccttcc caggtggagg ctaatttaat taatacgcgc tctttgctaa tgccttgggc
      841 tgcgtataaa tcaattaatt tgtgcgcttt ggcgatgctc gcttcggtgt cgaaggataa
      901 acgggcgtcc acttcagtgg atacgcggcc gggcacaatt ttcaaaattt cacagccgat
      961 ggctacacca attttatctg cggcatcgcc aatggatgcg gtgctggcga gggtttgttt
     1021 gaccagatcc tgatattgcg gcatttgcgc ggctttaaat aacagcgacg ggttggtggt
     1081 ggcatccagt ggctggtagc gggcgatggc ttcgatatca ccggtatcgg ctaccacgtc
     1141 ggaaaactgt tttaattgca cgagtttgtt ggtcatagaa cccccttaaa atggtcgttg
     1201 attatgggtg ttgcgccaaa gagtggcaac cgcccgttgg tgttggttgg gtaaatgcca
     1261 gggcggcccg taatacatcc agccctgcgg tgggtttatt ggcctcttca cttaaatgac
     1321 ggcgaaacag tcggccgccg ggctgggcgc taaataggcc gagcaaatgg cgggtaatgg
     1381 cgtgtaaccg gccccccgca gctagatggg cctcaatata aggctccaag cgggcgagaa
     1441 tttccccccg cgcggctatt ggggcctcgt cattaaataa tgttccatcc acctgggcga
     1501 gcaaataagg attgtagtac gcctcgcggc cgagcatgac gccatcgagc tgttcaaggt
     1561 gggttgcaca ttgctctaaa gtggtaatac cgccgttgac cacaaaggtt aatgccggaa
     1621 acgccttttt aagtgccacc acccgctcgt attgcagcgg tggaatttca cggttttctt
     1681 ttgggcttag gcccttaagc caggccttgc gggcgtgcac gataaacacc tggcagcccg
     1741 cctgggtatt aagcgccaca aagcggtgta ggtgttcatc cgcatccaac tcatcaatgc
     1801 ccagccggtg tttgatggtg atgggaatgg atgtagtgct gatcatgcgg tggacggcat
     1861 cggccactaa ttcaggttcc gccatcaaac aggcgccgat gcggccctcc tgcacccgat
     1921 ccgacgggca gccgcaattc aaattcacct cactaaaacc tgcatcctcc gccatgcggg
     1981 cgcacaggct taagtcctgg gcgctatggc caccgagctg cagagccagg ggtaactccg
     2041 ccgggtggta gtgtaaaaac cgcgcccgat tgccgtgaat cagcgcgccg gtggtgacca
     2101 tttcggtata cagccgggtg tgacggctca tgaggcgcca aaaataacgg gtgtgggcgt
     2161 cactccattc catcatagga gcggtgcaaa aacgccaggg gttaaaagag gtgtcggtgg
     2221 agtgcacgct gaattcttaa gtgaaaggcg ctattttgcc agagcttggc agcagagccc
     2281 aggtttgtgt gcttttttat tgatgaaaaa aacaggcctt atggttaaac ctaaaaaccg
     2341 cagttgaact ctactacggc tgcctaatgc ttgaatct
//
LOCUS       DAOQGS010000632         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_29371_length_2359_cov_3.964410, whole genome shotgun sequence.
ACCESSION   DAOQGS010000632 DAOQGS010000000
VERSION     DAOQGS010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29371_length_2359_cov_3.964410"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..733)
                     /gene="nth"
                     /locus_tag="PKZ52_17140"
     CDS             complement(104..733)
                     /gene="nth"
                     /locus_tag="PKZ52_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNG61552.1"
                     /translation="MVLAQLQERYPNPPIPLQHKDAFTLLIAVLLSAQCTDERVNQVT
                     PALFALADTPAAMARQSVEDIAAIIRPCGLSPQKSKAIKRLSELLIEHHQGQVPQDFA
                     ALEALPGVGHKTASVVMSQAFGVPAFAVDTHIHRLAQRWGLTSGKNVAQTEADLKALF
                     PQEHWNQLHLQFIYWGREFCSARGCDGTVCELCRACYPNRRKPKATQKA"
     gene            1079..2323
                     /locus_tag="PKZ52_17145"
     CDS             1079..2323
                     /locus_tag="PKZ52_17145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG61553.1"
                     /translation="MQHPSSTPALFTGYQKAVILVLALLQFLVILDFMIIAPIGDLLM
                     KTLNISTEQFGLVVSCYAFSAALSGIAFAGFADKFDRKKLLMTFVAGFIAATFLCGLA
                     NSFIELLAARILTGIFAGLCSSTLFAVVADIFAAQTRGRVMGMIQMGFGLSQVLGIPL
                     GLYIATHFNWHSTFMLIVAMSALVLLAIALVFQPIAEHLKQAVDKNAFLHLWHTLNNK
                     QYQIGFIATMFLTIGGFMLMPFSSVFLVNNVHISHEQLPIIFLCTGVSSLVMMPLIGK
                     MSDSYDRFRIFMFASIAACCMTVIYTHLTPIPFWIMVVFNMVFFAIIMSRMSPAMALN
                     SMVAKPQDRGAYMSISSSLQQTAGGLSAVVAGWVVYQPSSSSPIEHYDILGYMAVSVF
                     LLSVYLVRRVDLSLKQRQMAAA"
BASE COUNT          502 a          534 c          650 g          673 t
ORIGIN      
        1 gtgggtgggc gctcgggggc ggcccacaca ggcagggtgg gcagtaaaaa ggcggcaata
       61 aaaacagcta aacgcatggg aaaaccttag gttgggccag ctactacgct ttttgcgtag
      121 ctttaggctt gcggcgattg ggataacagg cccggcacag ctcgcatact gtgccatcgc
      181 agccccgcgc ggaacaaaat tcccggcccc agtagatgaa ttgcaaatgc agttggttcc
      241 aatgttcctg ggggaagagg gcttttaagt ccgcctcggt ttgcgccaca tttttccccg
      301 aggtcagccc ccagcgctgg gctaagcggt ggatgtgggt atccaccgca aaagccggta
      361 cgccaaaggc ttggctcata acgacgctgg cggttttatg gcctacgccg ggcagggctt
      421 ccagggcggc aaaatcctgc ggcacttggc cctggtggtg ttcaatgagc aattctgaca
      481 ggcgtttaat ggctttggac ttttgcgggg ataaaccgca ggggcgaata atcgccgcta
      541 tgtcctctac cgattggcgc gccatggccg ctggggtatc ggccagggca aatagtgccg
      601 gggtgacctg gttgacccgc tcatcggtgc actgggcgct gagcaacacg gcaattaaca
      661 gcgtaaaagc gtctttgtgt tgcagtggaa tgggtgggtt ggggtagcgc tcttgcagct
      721 gggctagcac cagtgcggcg cgttgccgtt tgttagggga ggcctgtttg gcgctcttgg
      781 cttctgagct ggaacgaaaa cgggaaacgg acactcaaca ggcctcggaa gaaaaggcag
      841 cattgtagtc ccattagatg cgagctggaa attgtctggc ggcaatgttt tggttttatt
      901 agcggcagcg atttttaatg tgagtattct atgtaattgt atttttgacc gtatatattt
      961 accggatgtc tgataaattg gggtttttaa aaccctcgac acgtcctttt ttacgagaat
     1021 cctgtgtgca aggcttaagc gcgttgcaac acttttactt cccattaggt tattcacaat
     1081 gcaacaccct tcgtctaccc ccgccttatt taccggctac caaaaagccg tcattttagt
     1141 tttggcgctg ctgcagtttt tagttatttt ggattttatg atcatcgccc ctatcggcga
     1201 tttactgatg aaaaccttga atatatccac cgaacaattc ggcttggtgg tctcctgtta
     1261 tgcctttagc gcggcacttt cgggaattgc ctttgccggt tttgccgata aatttgaccg
     1321 caaaaaatta ctcatgacgt ttgtggcggg ttttattgcg gctacttttt tatgtgggct
     1381 ggccaatagt tttattgaat tattggcggc gcgaatattg accggaattt ttgcaggcct
     1441 gtgcagttca accctgtttg ccgtagtggc ggatattttt gccgcgcaaa cccgtggccg
     1501 ggtgatgggc atgattcaaa tgggctttgg tttgagccag gtgttgggga ttccgctggg
     1561 cctgtatatc gccacgcact ttaactggca cagcacattt atgctgattg tggccatgtc
     1621 ggcgcttgtg ttgttggcga ttgccctagt atttcagccc attgccgagc atcttaaaca
     1681 ggccgtggat aaaaatgctt ttttgcattt atggcatacg ctgaataata aacagtacca
     1741 aataggcttt attgccacca tgtttttaac catcggcggc tttatgctga tgccctttag
     1801 cagcgtattt ttagtcaata atgtgcacat cagccacgag caattgccga ttatattttt
     1861 gtgcacggga gtatcttctc tggtgatgat gccattgatt ggcaaaatga gtgacagcta
     1921 cgaccgcttt cgaattttta tgtttgcctc tattgcagcc tgttgtatga cggttattta
     1981 cacccattta acccccatcc cgttttggat tatggtggta tttaatatgg tgttttttgc
     2041 catcatcatg agccgcatgt cgcccgccat ggcgttaaat tctatggtgg caaagccgca
     2101 agatcgcggc gcttatatga gtatttcatc atcgctacaa caaaccgccg gtggcttaag
     2161 tgcggtagtg gccggttggg tggtttatca gccctcctcg tcaagcccga ttgagcacta
     2221 cgatattttg ggttacatgg ccgtgagtgt ttttttgttg agcgtgtatt tagtgcggcg
     2281 agtggattta agtttaaaac agcggcagat ggcagcggcc tagggaacct ctgaaaaatg
     2341 taggcggcga agcgctggc
//
LOCUS       DAOQGS010000633         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_29498_length_2353_cov_4.266754, whole genome shotgun sequence.
ACCESSION   DAOQGS010000633 DAOQGS010000000
VERSION     DAOQGS010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29498_length_2353_cov_4.266754"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKZ52_17150"
     CDS             complement(<1..374)
                     /locus_tag="PKZ52_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNG61554.1"
                     /translation="MLKKTKLSSAIVSLMATTMFSAQHVYAEEAAEEVVVTGIRGALE
                     KAMDIKREAVGVVDAISAEDMGKFPDTNLAESLQRITGVSIDRSNGEGSKITVRGFGG
                     DNNMVTLNGRMMPAAGVYGGGSG"
     gene            complement(1430..>2353)
                     /locus_tag="PKZ52_17155"
     CDS             complement(1430..>2353)
                     /locus_tag="PKZ52_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HNG61555.1"
                     /translation="VTGDQKARLELLSGHGGIINALVFTDSQGLQHNVIAGFNSLQAI
                     EQDQYYRGVPLYPFVNRLDAGRYSFAGRSYQFPVNESARDNALHGFIQHLPVHISASH
                     ISPTRAQAQAVYNYPGDRAAYPFPARITMTYTLDSAGTLELEMAVQNLSSVEVPVGIG
                     WHPYFTLGEAVDDLSLQIPPSKRVLVDERLLPTGALDEQQLFPVLAPIAGTAFDTCYR
                     LDAAEDSGRSVQEVILWSERARQGLALWQISGKGQFNYLQICIPPDRASIAIEPVSCG
                     INAFNTHEGITILQPGAFFIATCGVRWLEHT"
BASE COUNT          640 a          596 c          549 g          568 t
ORIGIN      
        1 ccactaccac caccgtatac gccagcagct ggcatcatac ggccgttgag ggtgaccatg
       61 ttgttgtcgc cgccgaaacc gcgaacagtg attttagaac cttcaccatt ggaacggtca
      121 atagacacac cagtgatacg ctgaagagat tcagcaaggt tagtatcagg gaatttaccc
      181 atgtcttccg cgctaatagc atcgaccaca cccacagctt cacgcttgat gtccatggct
      241 ttttctaaag caccgcggat accagtaact acaacttctt ccgctgcttc ttccgcgtaa
      301 acatgctgcg cagagaacat ggtagttgcc attagcgaca caattgcgct tgataattta
      361 gtctttttta acatttgatt ctgactcgcc ttatttaggt tcaagggtga gggtggacgg
      421 atcgttggga ctacagacac ttattagcgt tatttttact gtcattgcac agcaagccta
      481 acgaaataat aaccaaacgg acgaggtgcc aaccaaaatg cggtacaaca tatcgcctgt
      541 taagcgtgta tctgaggtgt ttcattcttt cttggtttgg gatgaggctt cagtttcagc
      601 ccaccataca atagaccagt taacccaaaa gtgttaacgc cctacccaat ggctttttga
      661 aacttcattt caccgcgata cgattgatat ttcgcgcaag accaagacga ctgagaaccc
      721 ttctgatcgc cattgccatg tttactttta ttgtttttta gattctccct cctggccaaa
      781 gccaggcgga agcaaaccat ttccaaccag ttgccaggtt gcccaccgcg caatttaaga
      841 cacgtgaagc actctcatac aagctggcct atagcaaacg atgccacatc gggacttaaa
      901 agaaaagagg ttggcggctt ctacacgaaa tgtttctaat cgttttagcc gccaacagag
      961 aaaattcgca aaattgtctc aataattcga attggttggc tttacttttc aaccgatcaa
     1021 ttagttgggc aacgtaaaaa gtgtccgcct gttcagaaaa agaccatttt ttccgaccgg
     1081 aaccagcatg cgtcagcaat ttcaaaaaga attaaccttc aacaaaaggt caaaaaacga
     1141 tctctttggg caattttaaa tctcccaaat tctctttgct gcaataatat gaacctattc
     1201 catagcattg acttaccagg ctgccttcaa ctgcgaagtt gaaaaatagc actcatcact
     1261 taagcgcatc ttgccatccc caaaaagaac aacaaacatc cacaaagaat attttttgaa
     1321 caggaatatg atgcagcgca acagatgtac gcacttattt aagagtgcga taagcatgtg
     1381 acgataaaaa acggcgcttg agcgccgttt gggaggttag gcgcaattct taagtatgct
     1441 caagccaccg cacaccacag gttgcaataa aaaaggcgcc gggttgtagg attgtgatcc
     1501 cctcatgggt gttaaaggcg tttatcccgc agctaacagg ctcaattgcg attgatgcgc
     1561 gatctggagg gatacaaatc tgcaggtagt taaattggcc cttgccgctg atctgccaga
     1621 gagccaagcc ctgtcgagcg cgctcggacc acagaataac ttcttgcaca gaacgccctg
     1681 aatcctcagc cgcatcaagc cgataacagg tatcaaaggc tgtacccgca attggcgcca
     1741 aaactggaaa taattgctgc tcatccaggg cgccggtggg cagcaggcgc tcatcgacca
     1801 acacgcgctt agacggcggt atctgtaagc tcaaatcatc taccgcctcg cccagcgtga
     1861 aatagggatg ccagcctatg cctaccggaa cctccacaga cgatagattc tgcaccgcca
     1921 tttctagctc cagagttcca gcggagtcta gcgtgtaggt catagtgatg cgggcaggaa
     1981 aaggataggc cgccctatct ccagggtagt tgtataccgc ttgggcttga gcgcgagtgg
     2041 ggctgatatg ggatgccgag atgtggacgg gcaggtgttg aataaacccg tgcaaggcgt
     2101 tgtcgcgtgc agactcattg accgggaatt gatagctgcg gcctgcaaag ctatagcgac
     2161 cagcgtccag ccgattaacg aaggggtaca gcggcacacc gcgataatac tgatcctgct
     2221 cgatggcctg cagcgagtta aagcctgcaa taacgttgtg ctgcagaccc tggctgtccg
     2281 tgaataccag ggcgttgata attccgccgt ggcccgacag cagctccagg cgagcttttt
     2341 ggtcgcctgt gac
//
LOCUS       DAOQGS010000634         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_29546_length_2351_cov_3.647213, whole genome shotgun sequence.
ACCESSION   DAOQGS010000634 DAOQGS010000000
VERSION     DAOQGS010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29546_length_2351_cov_3.647213"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1826)
                     /locus_tag="PKZ52_17160"
     CDS             complement(240..1826)
                     /locus_tag="PKZ52_17160"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNG61556.1"
                     /translation="MSEPLLAVSHLSAQFTGAGAPCLLDVSFTLQAGKTLALVGESGS
                     GKSVTAQSIMRLFRPQQIHFDPQSRIDFNGQNLLALSERQLNRVRGAQIGFIFQEPMT
                     ALNPLHTIGKQIGEVIALHRGLRGAALQHAVVAALQQVQLDDAEQKYRAYPHQLSGGQ
                     RQRVLIAMALANQPQLLIADEPTTALDAQVQKHILDLLKTLQQDLGLTLLLISHDLPM
                     VRRYADAVAVMHKGRIVEQGPVEAVFNTPRSAYTQELLGVNEMPITPVPIAVDAAELL
                     QVANLNVDFVVQKSFWGKPLQFFPAVQQAHFTLRAGQTLGVIGESGSGKSTLVQALLR
                     LIPATGKVVLQGQDLFTLSAKALRPLRRHMQMVFQDPFSSLSPRLNILEIIAEGLQVF
                     EPNSQTIEAQVIEVMQAVGLDADLRYRYPHEFSGGQRQRIAIARALILKPQIIILDEP
                     TSALDRTVQRQVLLLLQQLQRQYGLAFILISHDYRAIKALSHQIAVMQRGKIVEYGAA
                     EQVFNQPQVEYTRQLLQAVY"
     gene            complement(1828..2307)
                     /locus_tag="PKZ52_17165"
     CDS             complement(1828..2307)
                     /locus_tag="PKZ52_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNG61557.1"
                     /translation="MKPVLFLLIILASIITPNFWWLLGLMLLFEWTSLVDVVRAEFLR
                     GRNLDYVKAAKALGVKDATLMWRHILPNATVATMTYLPFLITGAIATLTSLDFLGFGL
                     PPGSASLGELVAQGKNNLQAPWLALSAFGILASLLILLVFIGEGLRDAFDPRLVQGR"
BASE COUNT          625 a          604 c          557 g          565 t
ORIGIN      
        1 actaaggcgc tgaatatgcg cgcttttttc taaaaattgg gcgaaagcag tagtcaccac
       61 tacggtgagt ccaattttta gaaaaaagcg tgcagattca agtattagcc agccgcagta
      121 gagttcaact gcggttttta ggttaaatgg tcatcttggc atttttaacc tgtcatcctg
      181 cgcgcagtcg caggatccaa atagttgcat tttattacgt gtatttaaca agatggcagt
      241 taataaaccg cttgcaataa ttgccgcgta tactccacct ggggctgatt aaatacctgt
      301 tcggcagcgc catactccac aattttgccc cgctgcatca cagctatttg atggcttagg
      361 gcttttatgg cgcggtaatc gtggctgatc aaaataaatg ctaaaccata ctgccgctgc
      421 aactgctgca aaagtaataa cacctggcgc tgcaccgtgc gatccagggc cgaggtgggt
      481 tcatccaaga taataatttg cggttttaaa attaatgccc gcgcaatggc aatacgctgg
      541 cgctggccgc cagagaattc atgggggtag cgatagcgca gatcggcatc caaccccacc
      601 gcctgcatca cctcaataac ctgagcttca attgtttggc tattgggttc aaaaacctgt
      661 aggccttcgg caataatttc cagaatattt aagcgcgggc tgaggcttga aaatggatct
      721 tgaaaaacca tttgcatgtg gcgccgcaaa ggccgcaacg ccttggcact taaggtgaat
      781 aaatcctgcc cctgcaatac cacctttcct gtggctggaa ttaagcgcag tagcgcttgc
      841 accagggtgc ttttgccgct gccgctttca cctataacac ctaaggtttg cccggcccgc
      901 agggtaaaat gcgcctgctg cacagcgggg aaaaactgca gcggtttacc ccaaaaagat
      961 ttttgcacca caaaatccac atttaaattg gccacctgga gcaattcggc tgcatccact
     1021 gcgatgggca caggtgtaat aggcatttcg tttacaccta ataattcctg tgtgtaggcg
     1081 ctgcgcgggg tattaaatac ggcctccacc ggcccctgct ccacaatgcg gcccttgtgc
     1141 atcaccgcca ctgcatcggc gtagcggcgc accatgggta aatcgtggct aattaataac
     1201 agggttaaac ccaaatcctg ctgcagggtt tttaataaat ctaaaatatg tttttgcacc
     1261 tgggcatcta aagccgtggt gggctcatcg gcaattaata attgcggttg gttggccagc
     1321 gccatggcaa tcagcactcg ctggcgctgg ccgccggata actggtgggg ataagcgcgg
     1381 tatttttgct cggcatcgtc caattgcacc tgctgcaggg ccgccaccac tgcatgttgt
     1441 aaggccgcgc cacgcagccc tcggtgcagc gcaattactt caccaatttg tttaccgatg
     1501 gtgtgtaatg gatttaatgc cgtcatgggc tcttgaaaaa taaacccaat ttgcgcgccg
     1561 cgcacgcggt ttaattgccg ctcgcttaag gccaataggt tttgcccgtt aaaatcaatt
     1621 ctgctctggg ggtcaaaatg aatctgctgc gggcggaata aacgcataat actctgcgcc
     1681 gtgaccgatt tacccgaacc ggattccccc accaaggcca gtgttttacc cgcctgcagg
     1741 gtaaaactga catcgagcaa acaaggcgcc ccggcgccgg taaactgggc ggataaatgg
     1801 ctaacggcta atagcggctc actcatatca gcgcccctgc accaggcgcg ggtcaaatgc
     1861 atcgcgcagg ccttcgccaa taaataccaa taaaattaat aaactcgcca aaataccaaa
     1921 ggcacttaaa gccaaccaag gcgcttgcaa attatttttc ccctgggcga ctaattcgcc
     1981 taatgaggcg gaacccggcg gcaaaccaaa gcctaaaaaa tccagcgagg ttagcgtggc
     2041 tatggcaccg gtaattaaaa acggcaagta cgtcatggtg gccactgtgg cattgggcaa
     2101 tatatgccgc cacataaggg tggcatcctt aacgcccagg gctttagcgg cttttacata
     2161 gtctaaattg cggccgcgca aaaattccgc ccgcactaca tccaccaggc tcgtccactc
     2221 aaacaacaac attaaaccta ataaccacca aaaattcggt gtgataatac tggcgagaat
     2281 aattaataaa aataataccg gttttattta aatataaaag atatgaagaa ttcctcatgt
     2341 ttgttggagt t
//
LOCUS       DAOQGS010000635         2334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_29879_length_2334_cov_4.635805, whole genome shotgun sequence.
ACCESSION   DAOQGS010000635 DAOQGS010000000
VERSION     DAOQGS010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29879_length_2334_cov_4.635805"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKZ52_17170"
     CDS             <1..615
                     /locus_tag="PKZ52_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNG61558.1"
                     /translation="RDALKAFVDTSHDDDDFFLVAFNQRANLISEFTDGTTLANKLTL
                     VDTKGQTALYDAAYLGIEKVKQGRHQKRAILLISDGQDNSSRYTYGELRKLLKEAGVQ
                     IYCIGIVEMGGGAGGSLDLQGQGILEEIAQTTGGKYFRAHDPQELAKIYQELDTLEPI
                     EQEAAAYRPRQVLFFWPLGAALGIGLLLILMRLPIWRRFTVSPT"
     gene            640..>2334
                     /locus_tag="PKZ52_17175"
     CDS             640..>2334
                     /locus_tag="PKZ52_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNG61559.1"
                     /translation="MLESLQQFHFLRPWWFLALLPAAWLVWQLSRSQAQGDHWTRLIA
                     PHLLPFLLDGKGGEEKNRVGWLLLPLSLAVVALAGPTWQKLPNPVEKNTSALIVLWDM
                     SPSMLAQDIRPSRLERSRLKLIDLLKTRKDGLTGLIAFSGEAYVVTPLTEDVQTIINL
                     LPSLGPTTLPSVGSNPEMALSEAKKLLKAGNLEHGDILFLTDGIDSKAFANLDDQLKG
                     GKDRVTIWGVGTEEGAPIPLPRGGFLQTGKDLVIAKLNTAELKRFAEQHKGYYLPALA
                     DDGDIKFLSQLLVHTDQTQVQSDKTFDQWTESGQYLSLALLPLCLWLFRRGSLLVIVF
                     SLCLANSEPAAAAAPTEEEVKPAAAPEPAWRKDLRNTWNGLWFTPDQQGARLLKQGDA
                     PAAAEHFANPAWQATAQYQAGNFDAAAKTFSQDKSATGLYNQGTSLLKKGDYDGAIKA
                     FDEALKLQPSYGAAQHNKDIAVKLKALKAKQPKQNNGQKDPNQKQNGDKNQQQNGDSQ
                     NSDGQQGEGEKGEGKQGDQQQSDEQNQADKDKQNGADGEPKEPQQGQDQQGQKDQQE"
BASE COUNT          583 a          720 c          614 g          417 t
ORIGIN      
        1 cgcgatgcgc tgaaagcctt cgtggataca agtcacgatg acgacgattt tttccttgtc
       61 gcctttaacc aacgcgccaa cttaatctct gaatttaccg atggcacgac gctggccaat
      121 aagctgacgt tggtggacac caaagggcaa acggccttgt acgacgccgc gtatctgggc
      181 atcgaaaagg tcaaacaggg ccgccaccaa aaacgcgcaa ttctgcttat ctccgatggc
      241 caggacaatt cctcgcgtta cacttacggc gaattgcgca agctgttaaa agaagcaggc
      301 gtgcaaatct attgcatcgg gatcgttgaa atgggtggcg gcgcaggcgg ttcgctcgac
      361 ctgcaaggcc agggcattct cgaagaaatc gcccagacga ccggcggcaa gtatttccgc
      421 gcccacgacc cgcaagagct ggccaagatc taccaagagc tggacaccct tgagcccatc
      481 gaacaggagg ccgccgccta ccggccgcgc caggtgttgt ttttttggcc gctgggcgca
      541 gccttaggga taggcctgct gttaatcctg atgcgcctgc ccatttggcg ccgcttcact
      601 gtctccccta cttaactttt ttagaaggta aaagcccgca tgctcgagag cttgcagcag
      661 tttcattttt tgcgtccctg gtggttttta gccctgctgc ccgccgcttg gctggtgtgg
      721 caactctccc gcagccaagc ccagggcgac cactggacgc gcctgatcgc cccgcattta
      781 ttgccgtttt tattggacgg taagggtggc gaagaaaaaa atcgcgtggg ttggctgctg
      841 ttgccgctca gccttgcggt ggtggccctc gctggcccca cctggcagaa gctgcccaac
      901 ccggtggaga aaaacacctc cgcgctgatc gtgctgtggg atatgtcgcc gagcatgttg
      961 gctcaggaca ttcgcccctc ccgcttagag cgctcgcgct taaaactcat tgacctgtta
     1021 aaaacccgca aagacgggct cacaggcttg atcgccttct ccggtgaagc ctatgtggtg
     1081 acgcctctaa ccgaagacgt acaaaccatt attaacctac tgccaagcct cggccccacc
     1141 accctaccaa gcgtgggctc caacccagag atggccctaa gcgaagcaaa aaaactgctt
     1201 aaagcgggca acctggagca tggcgatata ttgttcttaa ccgatggcat agactccaaa
     1261 gcctttgcca acctggacga ccagctcaaa ggcggcaaag atcgcgtcac catctggggt
     1321 gtgggcacag aagagggcgc ccccatcccg ctgcctcgcg gtggcttttt gcaaaccggt
     1381 aaagacctgg taatcgccaa actcaacacg gctgagctca aacgctttgc cgagcaacac
     1441 aagggttatt acctgcccgc cctggccgac gacggcgata ttaaatttct ctcccaactg
     1501 ctggtgcaca ccgaccaaac ccaggtgcaa agcgataaaa ccttcgacca atggaccgaa
     1561 agcggccagt acctaagcct tgcccttttg cccctgtgcc tgtggttatt ccgccgcggc
     1621 agcctattgg taattgtgtt tagcctctgc ctggccaatt ctgagcccgc cgctgcggcc
     1681 gcgcctaccg aagaagaggt aaaaccggct gccgcccctg agcccgcctg gcgcaaagac
     1741 ctgcgcaaca cctggaatgg cctgtggttt acccccgacc aacagggcgc gcggctgtta
     1801 aaacaaggcg atgcccccgc cgccgccgaa cactttgcca accccgcctg gcaggccacc
     1861 gcccagtacc aagcgggcaa cttcgacgcc gcagccaaaa ccttcagcca ggataaatcc
     1921 gccaccggct tgtacaacca gggtacgtcg ctgttaaaaa aaggcgacta cgacggcgcc
     1981 atcaaagcct ttgatgaggc cctcaagctg caacccagct atggagccgc ccagcacaac
     2041 aaggatatag ccgttaaact caaagccctg aaggccaaac aacccaaaca aaacaacggc
     2101 caaaaggacc ccaaccaaaa acaaaacggt gataagaacc agcagcaaaa cggcgattca
     2161 caaaatagcg acgggcagca gggcgaagga gagaaaggcg aaggcaagca aggcgatcaa
     2221 cagcagtccg acgaacaaaa ccaagccgat aaggacaagc aaaacggcgc tgatggcgag
     2281 cccaaagagc cccagcaggg ccaggatcaa cagggccaga aggaccagca ggag
//
LOCUS       DAOQGS010000636         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_29940_length_2331_cov_4.287346, whole genome shotgun sequence.
ACCESSION   DAOQGS010000636 DAOQGS010000000
VERSION     DAOQGS010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29940_length_2331_cov_4.287346"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..881)
                     /locus_tag="PKZ52_17180"
     CDS             complement(66..881)
                     /locus_tag="PKZ52_17180"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNG61560.1"
                     /translation="MVFVKTLIRLIGLCLLLASCAHQSERGAGPAAHQQEPMITEGLS
                     RAKQSEISPSDGLQRLIDGNNRFLTGGQLKRDLLAQAQSRGVSGQFPFASIVSCADSR
                     ADPALLFDQGISDIFVARVEGNVINADVLGSLEYAAKISGSKAIVVLGHNHCGAVKGA
                     CDGLKLGNFTQLINRISPAVKATPSIGGPDRSSKNYEFVDAVAVKNVELSLKAIRTGS
                     PLLKELENKGQIKIVGAMLDIETGKVTFLGEPIGPGVKTTIKTRRAEPPAAAE"
     gene            complement(1028..1753)
                     /gene="dnaQ"
                     /locus_tag="PKZ52_17185"
     CDS             complement(1028..1753)
                     /gene="dnaQ"
                     /locus_tag="PKZ52_17185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNG61561.1"
                     /translation="MSRQIILDTETTGLEPQQGHRIIEIGCVELVNRKLTGRHFHHYI
                     NPQRDIDEGAQAVHGISAEFLADKPLFAQLAEAFFEFVKGAELIIHNAPFDVGFINHE
                     FNLLDKGFGKLEDHCTIIDTLAMARHRHPGQKNNLDALCKRYEIDNSHRELHGALLDA
                     EILADVYLAMTGGQITLSLGGEGQNADGSYAEKIIRVQRSKPGVIIKASAEELAEHEK
                     KLAQIDKASGGNCLWLKPEFSHY"
     gene            complement(1750..2187)
                     /gene="rnhA"
                     /locus_tag="PKZ52_17190"
     CDS             complement(1750..2187)
                     /gene="rnhA"
                     /locus_tag="PKZ52_17190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNG61562.1"
                     /translation="MKQIEIFTDGACKGNPGPGGWGALLRYQGTEKSLKGAEANTTNN
                     RMELMAAICALQAVREPCEITLTTDSQYVRKGVTEWMAGWKRNGWRTADKKPVKNQDL
                     WQALDAAAAVHRMHWRWVKGHAGHRENEIADQLANQAITELLS"
BASE COUNT          544 a          625 c          573 g          589 t
ORIGIN      
        1 agattcaagc attaggcagc cgtagtagag ttcaactgcg gtttttaggt taaatggcgt
       61 cggcattatt ccgccgcagc tggcggctct gcccggcggg ttttaatggt cgtttttacg
      121 cccggcccta tgggctcccc cagaaaggtg accttgccgg tttcaatatc cagcatggcg
      181 ccgactattt tgatctgacc tttgttttcc agctctttta acagcgggct gccggtgcgg
      241 atagccttaa ggctcagctc aacattttta actgccacag catcgacaaa ttcataattt
      301 tttgacgagc gatcgggccc gccgatgctg ggcgtagctt ttacagcggg gctaatgcgg
      361 ttaattaact gcgtgaaatt gccgagcttc aaaccatcac aggcgccttt taccgcgccg
      421 caatggttgt gacccagcac cacaatagct ttagagccgc tgattttcgc cgcatattcc
      481 aggctgccga gcacatcggc attgatcaca ttgccctcca ctcgcgccac aaaaatatcg
      541 ctaatgccct ggtcaaataa taaggctggg tcagcgcgag aatcggcgca actgacaata
      601 ctggcaaagg gaaattggcc cgatacaccg cgcgactgcg cctgggccag cagatcgcgt
      661 tttaattggc cgccggttaa aaagcgatta ttgccatcaa ttaaacgctg caacccatcg
      721 cttggagaaa tttcactctg tttagcgcgg ctgagcccct cggtaatcat aggctcctgc
      781 tgatgggccg ctgggcccgc accgcgctcg ctctgatgag cacaactggc gagcaaaagg
      841 caaaggccga ttaagcggat aagggttttt acaaatacca aaagaattcc tctacatcta
      901 aatattaatg ctactttgct ttgcatttaa tgcgcttaat tcagctgagc aaggcgcgcc
      961 acgaagctgc cgcgcgccac ctattcttaa cccggcacta tatgccagcg gataaatata
     1021 agagcactta ataatgacta aactccggtt ttagccacag gcaattaccg ccggaggcct
     1081 tatcaatttg ggcgagtttt ttctcgtgct ccgctaactc ctcggcactg gctttaataa
     1141 ttacgccggg cttgctgcgt tgcacgcgaa taattttttc cgcataggag ccgtccgcat
     1201 tttgaccttc accacctaaa gacaaggtaa tttgcccgcc ggtcatggcc aaataaacgt
     1261 cggcgagaat ttccgcatcc agcaaagcgc cgtgtaattc gcggtgggaa ttatcaattt
     1321 cgtagcgctt acacagggca tccaaattat ttttttgccc gggatggcgg tgccgcgcca
     1381 tggcgagggt atcaataatg gtgcagtgat cctctaactt gccaaagccc ttatccaaca
     1441 aattaaattc gtgattgata aaccccacgt caaacggcgc gttgtgaata attaattcag
     1501 cgcctttcac aaactcaaaa aacgcctccg ccaattgcgc aaacagcggt ttatcggcca
     1561 aaaattccgc gctgatgccg tgcaccgcct gggcgccctc atctatatcc cgctgcgggt
     1621 ttatatagtg gtgaaaatgg cggccggtca atttacggtt cactaattcc acacagccaa
     1681 tttcaataat acggtgcccc tgttgtggct caagaccggt ggtttcagta tcgagaataa
     1741 tttgacggct catgacagta actccgtgat ggcttggtta gctaattgat cggcaatttc
     1801 gttttcgcgg tggcccgcgt ggccttttac ccagcgccaa tgcatgcggt gcacagcggc
     1861 agcagcgtct agtgcctgcc acaaatcctg gtttttaacc ggttttttat cggcggtgcg
     1921 ccagccattg cgcttccacc ccgccatcca ctccgtcaca cccttgcgca catattgaga
     1981 gtcggtggtg agggtaattt cgcagggttc ccgcacggct tgcaaagcgc aaatagccgc
     2041 cattaattcc atgcggttgt tggtggtgtt agcttcagcg cccttgagag atttttccgt
     2101 gccttggtag cgcaacagtg cgccccagcc gccagggcca gggtttccct tacaggcgcc
     2161 gtcggtaaaa atttcgatct gtttcaaacc atacctatat atagaagaag gaaaacgggc
     2221 ggcctattcg ttgccgccgc tattggattt tttatctaaa aaacgcagtt gaactcgtag
     2281 cgagggcgcc taaggcgctg aatatgtgcg ctttgggcag ctgtaataga g
//
LOCUS       DAOQGS010000637         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30354_length_2312_cov_3.530793, whole genome shotgun sequence.
ACCESSION   DAOQGS010000637 DAOQGS010000000
VERSION     DAOQGS010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30354_length_2312_cov_3.530793"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1119
                     /gene="hflX"
                     /locus_tag="PKZ52_17195"
                     /pseudo
     CDS             46..1119
                     /gene="hflX"
                     /locus_tag="PKZ52_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
     gene            1157..2311
                     /gene="hflK"
                     /locus_tag="PKZ52_17200"
     CDS             1157..2311
                     /gene="hflK"
                     /locus_tag="PKZ52_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNG61563.1"
                     /translation="MAWNEPGKNDNDPWGTNSRGKNDGPPDFDELLRSAANKLGRLFG
                     GSGGGGNNASDPSSLAPFIALAGVLAVLIWVGSGFRVVNEQERAVVLRLGSYLETVGP
                     GFRWNPPLIDRVLTENVTRVRLFTTDEQMLTKDLNIVDIKLSVQYRIQDARAFILNVD
                     QPEETLKQATSSALRHVVGTTLMHNVLTEGRAQLGVDVEQRLQTYLGNYTTGIDIEKV
                     NIEDSNPPKEVQTAFDDVIRAREDEERFKNVAQTYANDVIPSAGGAAKRMVEEAMAYS
                     SKVISEAEGEAKRFELLLAEYTKAPEVTRKRLYIDAIEEIMGKTTKVMVDVDKGNNMM
                     YLPLDKIIDAAPKPAAAGRPGMSASDLETITNQVLERLNREASTSRRGGR"
BASE COUNT          523 a          652 c          666 g          471 t
ORIGIN      
        1 ggattgttac gccggcacat cgcacggtga cggtgtatcg ctctccagtt ggtggtgttt
       61 aaccacagtc tctccccctc ccaagagcgc aacctcgagc gcgagcttaa ttgccgggtc
      121 atcgaccgta ccggccttat tctcgatatc ttcgcccaac gcgcccgcac ccatgaaggc
      181 aagctgcagg tggagctggc ccagctgcac cacatgtcca ctcgcctggt gcgtggctgg
      241 acccaccttg agcgccaggg cggcggcatc ggcctgcgcg gccccggcga aactcaatta
      301 gaaactgatc gccgcctact gcgcgggcgt ataaccatgc tggaaaagcg cctggaaaaa
      361 gtgcgccggc agcgcgagca ggggcgcagg tctcgtctgc gggccgacat accgacggtc
      421 tccttagtcg gctacaccaa tgcgggtaaa tccaccctgt tcaacagtat tactaaggcc
      481 ggggtgtacg ccgaaaacca gttgttcgcc accctcgacc ccaccatgcg ccgggtggat
      541 attcccggtc tcggcaaggt ggtgttggcc gataccgtag ggtttatcag ccatttgccc
      601 cacaagttgg tggaagcctt tcgcgccacc ctagaggagg cggccagcgc tgacttgctg
      661 ttgcacgtaa tcgacgccgc cgacccggag cgcgcgcgca acatcgaaca ggtggagaag
      721 gtgctcggcg atattcaggc tttggatatt ccgcgcctaa acctgtacaa caaaattgac
      781 ttgctgcggg atatggaacc gcgcattgat caggacgata gcggatcggc agtagcggtt
      841 tggttatctg cccaagcggg tagcgggctt gagctattaa gtgcggccat agcgcaaaag
      901 ctcggccacc agatctgcag gggtgcggtg cgtttaaaag cctcccaggg cagcctgcgg
      961 gccaagctgc acagcgcgca agcggtccag aatgaagtct ataacgagga cggtagtatc
     1021 caaattagcc tgtgtttgcc acagatggat ttaaaccgta ttctcgccgc cgaattgctc
     1081 actccagacc aattggaatg gctcaacacc cagccataac aatctctatc cttttaattg
     1141 cattggagtt ttaaccatgg cctggaatga accgggtaaa aacgataacg acccctgggg
     1201 caccaattcc cggggcaaaa atgacggtcc gcccgatttc gacgagctgt tgcgcagcgc
     1261 ggccaataag ctggggcggt tgtttggcgg cagtggtggt ggcggcaata atgcttccga
     1321 tccctccagc ctcgctccct ttattgctct ggcgggtgtg cttgcggtgc tgatatgggt
     1381 gggcagtggc tttcgggtgg tgaacgagca ggagcgcgcc gttgtattgc gcttgggttc
     1441 ttatcttgaa accgtggggc cggggttccg ctggaatcca cccttgattg atcgggttct
     1501 aaccgaaaat gtcactcgcg tcaggctctt taccaccgat gagcagatgc tcactaaaga
     1561 cctcaatatt gtcgatataa aactctctgt gcagtaccgc atccaggatg cccgcgcgtt
     1621 tatcttgaac gtagaccagc cggaggagac cctcaagcag gctaccagca gtgctttgcg
     1681 ccatgtggtg ggtacaaccc tgatgcataa cgtgctcact gaaggccgcg cccaattggg
     1741 ggtggatgtg gagcagcgcc tgcagaccta tctcggcaat tacaccaccg gtatcgatat
     1801 cgagaaggtc aatatcgaag actccaaccc gcccaaagag gtgcaaacgg cgtttgacga
     1861 tgtgattcgc gcccgcgaag acgaagagcg ctttaaaaac gtcgcgcaaa cctacgccaa
     1921 cgacgtaatc cccagcgcag gcggtgccgc caagcgcatg gtggaagaag ccatggccta
     1981 tagctccaag gttatttctg aggcggaagg tgaggccaaa cggtttgaat tgttgctggc
     2041 cgagtacacc aaagccccgg aagttacccg caagcgtctc tacatagatg ccatcgaaga
     2101 aatcatgggt aaaaccacca aagtaatggt ggatgtggat aagggtaata acatgatgta
     2161 cctgcccctc gataaaatta tcgatgcggc ccccaagcct gcagcggcag ggcgtcctgg
     2221 tatgagcgcc tcggatcttg aaaccataac caatcaggtg ttggagcgac tcaatcgcga
     2281 ggccagcacc tcccgccgag gaggccgcta at
//
LOCUS       DAOQGS010000638         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30376_length_2311_cov_4.770390, whole genome shotgun sequence.
ACCESSION   DAOQGS010000638 DAOQGS010000000
VERSION     DAOQGS010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30376_length_2311_cov_4.770390"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..2152
                     /locus_tag="PKZ52_17205"
     CDS             155..2152
                     /locus_tag="PKZ52_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNG61564.1"
                     /translation="MRLSTKNGLLAGVLLCVMLLMAATGYWSGSRLSSILAYITGPAW
                     DTADGAMETTIGIQGEMGWMQSRLLGVPVTDAQLATATQMTDEAFARLLAAQIMDKNA
                     SDKVAIELKLYRAAFAKLRAEYDKMQALQQKLQANVEPMMRLGAAMEELGDQQFEALE
                     AQASISVASFKNRWSAADGGMESLIGFYSQLYYLEQLIHGADFTPLPDKINQALGIQQ
                     AAVKAMVESGVFDSPIKDFGKEPAAKAYADYFAQHQSLMTQTVTSALAFRQQFKQCEE
                     ASGVLLASLGAFEELGDSAVEGQTANVATIIKFAQYATIFMLLLVTVTVAGNYFMLKI
                     WVLFPLDLLQTRINDLVRGEGDLTARIDHLRQDELGDLGRSINQLMVLLHALVVKIND
                     KGRAIEAKIAFNKSVAVSTFNHTKNVAENAQNLAAASGQVFVAAGAIASACTSASVSV
                     SQARSNTSSSRDTTRNTAMGMDRVCRLVNELSARITQLRDSAETIGKIVSVIGGISDQ
                     TNLLALNAAIEAARAGEQGRGFAVGADEVRTLAKRTSQSTVEITEVIRAIQDMSQSAF
                     ELINTCTHEVNSKAQDSQSVCSSLEQLAHVVDDLSGMIEQAASAAEEQAAVTKDMSQR
                     VQVIANDAAGVDMEARASMSCADELNQLARQLNQELARFRV"
BASE COUNT          536 a          532 c          691 g          552 t
ORIGIN      
        1 gatgtgtatt cactgcgggt gcttaggtag taagtctgca ggcggcgcag gttttattct
       61 tgctgcagat ttttaaagct ctcctgcctt tgtgtatctg aatacatatg gcatgtggtt
      121 gttaaaaaat gagtggaata tttgaggagc caatatgcgg ctatcgacaa aaaatggctt
      181 attagctggc gtattgttat gcgtaatgtt gttaatggcg gcgacgggtt attggagcgg
      241 tagccgcctc agcagtattt tggcctatat taccggcccc gcctgggata ccgccgacgg
      301 cgccatggaa acgaccattg gtattcaagg tgaaatgggt tggatgcaga gccgtttatt
      361 gggtgtaccg gtgacggatg cgcaattggc aaccgcgacg caaatgaccg atgaagcctt
      421 tgcccgttta ttggcagccc agataatgga taaaaatgcc agcgataagg tggctattga
      481 attaaaactc taccgcgccg ccttcgctaa attgcgcgcc gagtacgata agatgcaagc
      541 cttgcagcag aaattgcaag ccaatgtaga acctatgatg cgtttgggcg cggcgatgga
      601 ggaattgggc gaccaacaat ttgaggccct agaggcgcaa gcatccataa gtgtggccag
      661 ctttaaaaac cgctggagcg ctgccgacgg cggtatggaa agcctgattg gtttttacag
      721 ccagctgtat tatttggagc aattaattca tggggctgat tttacgcctc tgccggataa
      781 aattaaccaa gctctgggaa ttcagcaggc ggctgtaaaa gctatggtgg aaagcggtgt
      841 atttgatagc cccataaaag attttggcaa ggagcctgcg gctaaagcct atgccgatta
      901 ttttgcccag caccagagtt tgatgacaca aacggtaact agtgcgctgg cttttcggca
      961 gcaatttaaa cagtgtgaag aggcttcggg cgttttatta gcgtctttag gcgcgtttga
     1021 agaattaggt gatagcgcgg tagaagggca gacggcgaat gttgccacca ttattaaatt
     1081 tgctcagtac gccactattt ttatgttgct attggtgacg gtgacggtgg cgggcaatta
     1141 ttttatgctc aaaatatggg tgctgtttcc gctggattta ttgcagacgc ggattaatga
     1201 tttggtgcgc ggggaggggg atttaacggc ccgcatcgat catctgcgcc aggatgaatt
     1261 gggggattta ggccgcagta ttaatcagct catggtcctg ctgcacgctc tggtggtgaa
     1321 gattaatgac aagggccggg ccatcgaagc gaaaatcgct tttaataaat ctgtggcggt
     1381 ttccacgttt aatcacacca aaaatgttgc cgagaatgcg caaaaccttg ccgccgctag
     1441 cgggcaggtt tttgtcgctg caggcgccat tgctagcgcc tgtacgagcg cctcagtatc
     1501 cgtaagccaa gcgcgcagca atacctccag tagccgcgat accacccgca atacggctat
     1561 ggggatggat agggtgtgcc ggttggtgaa cgaattaagc gcgcgcatca cccagttgcg
     1621 cgatagcgcc gaaaccattg gcaagattgt ttcggtgatc ggcggtattt ccgatcaaac
     1681 caatttgctg gccctcaatg ccgccattga ggcggccagg gctggtgagc aggggcgggg
     1741 gtttgcggtg ggggccgatg aggtgcgcac cctggcaaag cgcacatccc agtccacggt
     1801 ggaaatcacc gaggtgatcc gtgccattca ggatatgtcc caaagcgctt ttgagttaat
     1861 taacacctgt acccatgagg tcaacagcaa ggcgcaggat tctcagagcg tgtgtagctc
     1921 attggagcag ctggcccatg tggtggatga cttatcgggc atgatcgaac aggccgccag
     1981 cgccgctgaa gagcaggcgg cggtgaccaa agatatgtcc cagcgggtgc aggtgatcgc
     2041 caatgacgcc gccggggtgg atatggaggc ccgcgccagt atgagttgcg ctgatgaact
     2101 caatcagttg gcgcggcagt taaatcagga gctggcgcgg tttcgggttt aattcaccgc
     2161 ccggctctgc cgagcggtta caccaccagg ctcacctgct cgccattcca ctggcaggct
     2221 tcgctcaaca aaaaaccata ggccggcgca atggattcgg cgctttttac cgtggccggg
     2281 tcttccgccg ggtaggcggc ggcgcgcatg g
//
LOCUS       DAOQGS010000639         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30412_length_2309_cov_4.245342, whole genome shotgun sequence.
ACCESSION   DAOQGS010000639 DAOQGS010000000
VERSION     DAOQGS010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30412_length_2309_cov_4.245342"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            498..1460
                     /locus_tag="PKZ52_17210"
     CDS             498..1460
                     /locus_tag="PKZ52_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61565.1"
                     /translation="MYRNFYGLTKPPFRITPDTQAFFAGSERGALLDGLCYAISQGEG
                     IIKLVGEVGSGKTMLCRMLPLRLAESIDWVYLAHPSLSPEHILHAIAQEMGMKVSPSA
                     DKLAVMRSLHKALLKRHMRNRQVVVLVEEAQGMLLETLEEIRLLSNLETCDHKLMQIV
                     LFGQPELDTKLAAPCIRQLRERITYSFYVQPFKQADIQAYLDFRLRAVGYTGPELFDA
                     ELVQLIERYSQGLVRRINILADKTLLAAYTQDRHRLLAADVHQAACDSQYNEPQVISW
                     ARQLKLKIKHRVPAWAALSGLMLAAGCWGATAAPFGLTVGSQLL"
     gene            1457..>2309
                     /locus_tag="PKZ52_17215"
     CDS             1457..>2309
                     /locus_tag="PKZ52_17215"
                     /inference="COORDINATES: protein motif:HMM:NF019280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNG61566.1"
                     /translation="MSRFLLRALCLIPLFATVSCDLLRTKPAQSTGHLSAPALDSARI
                     PALAAAPPRLPPPQPVKREATHTVVVSDVPVREVLFSLARDADVNLDLAEDVDGVVTL
                     NAVNQTLPAILDRIAANSGLRYELNNKVISIRKDLPFVRNYRVDYLNMARAASSNVSV
                     STQISATGQGAGDAKGAGSGDNNSATQVKNSSDNSFWATLQKNIAAIIGGGTPAAGAT
                     AEAGAGADHPDIMLNKESGIMGVRATERQHAEIARFIAEVQTSSERQVLIEATIAGDI
                     GFSVDKTI"
BASE COUNT          501 a          588 c          669 g          551 t
ORIGIN      
        1 gttagcgccc tttccaactt cccacccaga cgccccctta aatagggtga ggagaaaaaa
       61 actgcaaatc agggcattta ctagtgctcc agttgatttt gctccctccc cttgagaagg
      121 ggagggttgg tgtggggttt atgggtaagc gcaaaatcaa gtaaatacaa aacgcttaat
      181 aattacgttt caatccatgg gattgtcctg cacctgtaca ggcactcata tttttagcca
      241 atttgccctg ccagatcttc gccgcccagg catttcaccc ggctgatgaa ccgctggcca
      301 cgcctgagcc gccgctctgg cctttattcc ttcctgaact acactcaagc agatctagac
      361 ccagttcgcc taaccaaaac cccagcagca cttgctctat tggagcggat tgccctgagg
      421 ttctggccgc gtctattgtc cgcgctcctt ggtggttgcc tgttaaggag tttagatacc
      481 gcgagatttg aaacactatg tatcgcaact tttatggctt aactaaaccg ccgtttcgca
      541 taacgccgga tacccaagca ttttttgccg ggtcagagcg cggggcgctg ttggatggct
      601 tgtgttacgc catcagccaa ggtgagggaa ttattaaatt agtgggcgag gtgggcagtg
      661 gcaaaaccat gctctgtcgg atgttgccac ttaggttggc agagtcgatc gattgggtgt
      721 atctggcgca cccgagtttg tcccccgaac acatattgca tgccatagcc caggagatgg
      781 gcatgaaagt gagcccgtcg gcggataaat tggcggtgat gcgctcgctg cataaagcgc
      841 ttttaaaacg ccatatgcgc aaccggcagg tggtggtatt ggtggaggag gcccagggaa
      901 tgttattgga aaccctggag gaaattcgcc tattaagtaa tttagaaacc tgcgatcaca
      961 aattgatgca gatcgttttg tttggccagc cggagttgga taccaaatta gccgcgccct
     1021 gtatccgcca actgcgcgaa cgcatcacct atagttttta tgtgcagccg ttcaaacagg
     1081 cggatattca agcttatctg gattttaggc tgcgcgcggt gggctatacc gggccggaat
     1141 tattcgatgc cgagctggtg caattaattg agcgttattc gcagggctta gtgcggcgca
     1201 ttaatatttt ggcggataaa acgctgttgg cggcctacac acaagaccgc caccgtttgc
     1261 ttgctgcgga tgtgcatcag gccgcttgcg acagccaata taatgagccg caggttatct
     1321 cttgggcgcg gcaattaaaa ttaaaaatta aacacagggt gccggcttgg gctgcgttaa
     1381 gtggcttgat gttggcggct ggttgctggg gcgcgacggc ggcccctttt gggttgacgg
     1441 tgggcagcca gctgttatga gccgttttct cctgcgcgca ctgtgcctga tccctttatt
     1501 cgcaactgta tcctgcgatc tgctgcgcac taagcctgcg caatccactg ggcatttgag
     1561 cgcgcctgcg cttgatagcg cgcgcattcc ggcattggca gcggcgcccc cgcgcctgcc
     1621 gccgccccag cccgttaagc gcgaggccac ccatacggtg gtggtaagcg atgtgccggt
     1681 gcgggaggtg ttgttttcgc tggcgcggga tgcggatgtg aatttggatc tggcggagga
     1741 tgtggatggc gtggtgacgc tcaatgcggt gaaccagacg ctaccggcta ttttggatcg
     1801 catcgcggcg aattctgggc tgcgttatga actgaataac aaggtcatca gtattcgcaa
     1861 agacctgcca ttcgtgcgca attatcgggt ggattattta aatatggcgc gcgccgccag
     1921 cagtaatgtc agcgtttcca cgcagattag cgccaccggg cagggcgcgg gcgatgccaa
     1981 gggcgcaggc agcggcgaca ataattcggc cacccaggta aaaaacagct cggacaatag
     2041 tttttgggcg accctacaga aaaacatcgc cgccattatc ggcggtggta cgcctgcggc
     2101 gggggccact gctgaagccg gtgccggggc ggatcacccg gatattatgc tcaataaaga
     2161 aagcggcatc atgggggtgc gcgccaccga gcgccaacat gccgaaatcg cccgttttat
     2221 tgccgaggta caaaccagct cggagcggca ggtgttaatt gaggccacca ttgccggtga
     2281 cattggtttc tctgttgata agacgattt
//
LOCUS       DAOQGS010000640         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30443_length_2308_cov_3.536618, whole genome shotgun sequence.
ACCESSION   DAOQGS010000640 DAOQGS010000000
VERSION     DAOQGS010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30443_length_2308_cov_3.536618"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2308)
                     /locus_tag="PKZ52_17220"
     CDS             complement(<1..>2308)
                     /locus_tag="PKZ52_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61567.1"
                     /translation="LTVTFKIGTNADLAQQMVQNRVSQALPRLPEVTRQAGVTVVKSS
                     PDLTMVVHLISPDKSYDELYLRNYALMNIKDELAKVPGVGLVRLFGSGDYAMRIWLNP
                     DKIAQYNLTASDVTNAIREQNIQVAAGLIGGAPTTNLIDVQLPVNAKGRLSSPEEFGN
                     IIVRAGKQGELILLKDLARIEMSAADFNLAAQLDGQPAVAIPIFQSPGANAIQISDGV
                     RAKMAAIKNRLPAGIDYQVVYDPTIFVKSSIKAVIKTLLEALALVVVVVILFLQTWRA
                     SIIPLLAVPVSIIGTFALMWVFGFSINTLSLFGLVLAIGIVVDDAIVVVENVERNIED
                     GLSPLQATYKAMQEVSGPIIAISLTLIAVFVPIAFVSGLTGQFYKQFALTIAISTVIS
                     AVNSLTLSPALAALLLKSHSAPKDRLTRVMDRFCGRFFAAFNKVFSRSSGRYSTQVGK
                     LLGRKTLAVGIYAVFLAITAGEFLSIPKGFVPAQDKQYLIAFAQLPDGATLARTKEVI
                     KEMGDIGLKEAGVANAVQFPGLSINGFTNSASAGIVFFPLKPFDERKDKSMSAGAIAR
                     TLQMKFAGIPEAFIAIFPPPPVRGLGTTGGFKLQIEDRGGLGYERLAEVVAQVQQKAA
                     TRPELASVFSNFKINVPQLYADLDRPKAKQLGLNIKEIFDSMQIYLGSLYINDFNQFG
                     RTYQVIAQADAPFRNSPQDALNLQVRNTSGEMVPLGSVLSIKESFGPESAAHYNGYLA
                     ADLNGNAAPGYSSGQAQDAIEAILAETLP"
BASE COUNT          628 a          607 c          526 g          547 t
ORIGIN      
        1 ggtaaggttt ccgccaggat tgcctcaata gcatcctgcg cttgcccgct ggaatatccc
       61 ggtgctgcat tgccatttaa atccgccgcc aaataaccgt tgtaatgggc ggcgctctct
      121 gggccaaaac tttctttaat actcaacaca gaacctaagg gcaccatttc gccactggta
      181 ttgcgcacct gtaaattcag cgcatcctgc gggctattgc gaaagggcgc atccgcctgg
      241 gcaatcacct gataggtgcg gccaaactgg ttaaaatcgt taatgtataa agagcctaaa
      301 taaatctgca tgctgtcaaa aatttccttg atattaagcc ctaactgttt ggctttgggc
      361 cgatccaaat cggcatataa ttgcggcaca ttgattttaa agttggaaaa cacactcgcc
      421 agctccggcc gggtagcggc cttttgttgc acttgggcga ctacttccgc caagcgctca
      481 taacctaagc cgccgcgatc ttcaatttgc aatttaaatc caccggtagt gccaagcccc
      541 cgcacgggcg gcggtggaaa tatggcaata aaggcctcag ggataccggc aaatttcatt
      601 tgtaaggtgc gcgcaatcgc ccctgcactc atgcttttat ccttgcgctc atcaaagggt
      661 ttaagcggga aaaacacaat gcctgcactt gcgctattgg taaaaccatt aatgcttaaa
      721 ccaggaaatt gcacagcatt ggcgacaccc gcctctttta agcctatatc acccatttct
      781 ttaattactt ctttggtgcg cgccagggtg gctccatcgg gcaactgagc aaaagcaatt
      841 aaatattgtt tatcctgggc gggcacaaaa cctttgggga ttgataaaaa ctcacccgcc
      901 gtaatggcca aaaataccgc ataaataccc accgccagag ttttgcggcc cagtaattta
      961 cccacctggg tggaataacg gccagagctg cgtgaaaata ctttattaaa ggccgcgaaa
     1021 aaccgcccac aaaaacgatc catcacacgg gtgagccgat ccttcggtgc cgaatggctt
     1081 tttaataata atgccgccag cgcagggctg agagttaagg agttaaccgc tgagatcacc
     1141 gtagaaatag cgatggttaa tgcaaactgt ttataaaatt gcccagttaa gccgctgaca
     1201 aaagcgatag gcacaaatac cgcaataagc gttaaggaga tggcaataat cggcccgctc
     1261 acttcctgca tagctttata agtagcctgc agtgggctta ggccatcctc aatattgcgc
     1321 tcaacgtttt ccaccaccac tatggcgtca tctaccacta tacctatggc caataccaaa
     1381 ccaaataaac tcaaggtatt aattgaaaag ccaaacaccc acatcaatgc aaaggtgccg
     1441 ataatcgaca caggcacagc cagcagcgga ataatagagg cgcgccaggt ttgcaagaat
     1501 aaaatcacca ccaccacaac cagagccaaa gcttctagca gagttttaat caccgcctta
     1561 atggagcttt taacaaaaat ggtggggtcg tacaccacct gataatcaat gcccgcaggt
     1621 aggcgatttt taatagcggc cattttggcg cgtacaccgt cagaaatttg aatggcattg
     1681 gcgccggggc tttggaaaat cggaatcgcc accgccggtt ggccatccaa ctgagcggcc
     1741 agattaaaat ccgccgcgct catttcaatg cgcgctaaat cttttaataa aattaactca
     1801 ccctgtttgc ctgcacgcac aataatattg ccgaattctt caggagagga cagcctgccc
     1861 ttggcgttta ccggcaattg cacgtcaata aggttagtgg tgggcgcgcc gccaattaaa
     1921 ccggctgcca cttggatatt ttgctcgcgt atggcgtttg ttacatcgct agcggtgagg
     1981 ttgtactggg caattttgtc cgggttaagc cagatgcgca tggcataatc acccgaacca
     2041 aataaacgca ccagccccac gccgggcacc ttggccaatt catctttaat attcatcagc
     2101 gcatagttgc gcaggtataa ctcatcgtag gatttatccg gcgaaattaa atgcaccacc
     2161 atggttaaat ccggcgagct ttttactacc gtaaccccgg cctggcgggt cacctccggc
     2221 aaacgcggca gcgcttggga tacgcggttt tgcaccattt gctgggccaa atccgcatta
     2281 gtgccaattt taaaggtcac cgttaggg
//
LOCUS       DAOQGS010000641         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30500_length_2305_cov_4.410222, whole genome shotgun sequence.
ACCESSION   DAOQGS010000641 DAOQGS010000000
VERSION     DAOQGS010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30500_length_2305_cov_4.410222"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKZ52_17225"
     CDS             <1..1020
                     /locus_tag="PKZ52_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat family protein"
                     /protein_id="HNG61568.1"
                     /translation="LVTAHLPDRDGDGTPDILDLDSNNDGRPDISQTPFASLDTNRDG
                     RIDSQNDSDQDGLDDAAGGDKQLRGSNTDSDGDGVPDSRDIDDDNDGIVDVLEGDGDA
                     DGDGIPNRLDRDSDNDGLSDRFEAKAAAPSGKDSDGDGLDDAYDPDAKGQADSDGDGI
                     ADSMDLDQTHGQDANHDGLDDALGKAAALLDTDGDGKPDAFDTDSDGDTLADNLESGL
                     IKPSGHDQDRDGIDDAFDADFTGGKDADGDGLDDASAAKTDLDRDGLLAYRDTDTDGD
                     GYSDTIENGDFNNDQVIDRQQPPGKMRTAVRGGGADGYLLLLAAMAMPLGRRRLKHTL
                     KRKTA"
     gene            1032..1610
                     /locus_tag="PKZ52_17230"
     CDS             1032..1610
                     /locus_tag="PKZ52_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNG61569.1"
                     /translation="MQSLHAIVLTALTCIAAQAQAAESDSRQPPDTLYGGLSLGLSQL
                     NPQENGSGWHTIDSSSTGFSLYLGYPINSQWFAELTYADLGAATVAPRNNALGGPQDI
                     SYQVPSLTAVYYFWQPKGDIYTYLRAGVASIINKNSGDTDIYEKNTSAQIALGLGAEW
                     RFAEDIFTRLEITSYDVDAQIISLSIGCWIND"
BASE COUNT          599 a          677 c          595 g          434 t
ORIGIN      
        1 ctcgtcaccg cccatttgcc cgaccgcgat ggcgacggca cgccggatat tctggaccta
       61 gacagcaaca acgacggccg ccccgacatc agccaaaccc cgtttgcaag cctagatacc
      121 aaccgcgatg gccgcatcga cagccaaaac gacagcgacc aagacggcct ggatgatgcc
      181 gccggcggcg acaaacagct gcgcggcagc aataccgata gcgacggcga cggcgtgccc
      241 gacagccgcg acatcgacga tgacaacgac ggcatcgtcg atgtgttaga gggcgatggc
      301 gacgcggacg gcgacggcat ccccaaccgc ttagaccggg acagcgacaa cgatggctta
      361 agcgaccgct ttgaagccaa ggccgccgcc cccagcggta aagacagcga cggcgacggc
      421 ctggatgacg cctacgaccc agacgccaag gggcaagccg atagcgatgg cgacggcata
      481 gccgacagca tggacctaga ccaaacccac ggccaggacg ccaatcacga cggcctggac
      541 gacgccctgg gcaaagccgc cgcattgctg gataccgacg gcgacggcaa acccgacgcc
      601 tttgacaccg acagcgacgg cgacaccctg gcggataacc tggaaagcgg cctaatcaaa
      661 cccagcggcc acgaccaaga ccgggacggc attgacgatg ccttcgacgc cgactttact
      721 ggaggtaagg acgctgacgg cgatggcctc gacgatgcca gcgccgccaa aaccgattta
      781 gaccgcgacg gcctgctcgc ctaccgggat accgacacgg acggcgacgg ttactcagac
      841 accattgaaa acggcgactt taataacgac caggtgatcg accggcaaca accgcccggc
      901 aaaatgcgca ccgccgtgcg cggcggcggc gccgacggct atctgctact gctggccgca
      961 atggcgatgc ccctgggcag acggcggcta aaacacactt taaaacgcaa aactgcctaa
     1021 agagggacac tatgcaaagc cttcacgcca ttgtgctcac cgcgctcacc tgcatcgcag
     1081 cccaggccca agccgccgaa agcgacagcc gccaaccccc agacaccctc tacggcggct
     1141 taagtctagg cctcagccaa ttaaacccgc aggaaaatgg cagtggctgg cacaccatcg
     1201 actcgtcctc cacaggtttt agcctttatc taggctaccc aatcaatagc caatggttcg
     1261 ccgaactcac ctatgccgac ctaggcgccg ccaccgtagc tccgcgcaat aatgccctcg
     1321 gcggccccca agacatcagc taccaagtgc cttcactcac tgctgtttat tatttttggc
     1381 agcccaaagg cgatatttac acctacttgc gcgcaggagt ggcgagcatt attaataaaa
     1441 acagcggcga taccgatatt tacgagaaaa acaccagcgc acaaatcgcc ctgggtttag
     1501 gggccgagtg gcgctttgcc gaagatatat ttacccgcct agaaattacc agttacgacg
     1561 tggatgccca gataatatcg ctgagtattg ggtgctggat taatgattaa attttggatt
     1621 ggtgattaac agcttgttga aatactagct tgccagcgcg gaagaggaga gtaaagagtg
     1681 ctgaatagtt acgaatttct agctagtgtg caggcgcaca aggacttaag ctgaacatcc
     1741 taaaaatggg gctggtgctt tttttagcct gttttcatgc ccgataaatt tccgctttct
     1801 tcatctgtat ccgcatataa aagagctgaa gtctattctc cgctaaaagc agtgcctaaa
     1861 attcccctcc tgtggagggg tgccccgaag gggtggggtg gtagggcgtc agtgtatttt
     1921 atggttacaa cctaccgcca cataatgaaa aaaatgtagc tattcgcagg ttttatgtag
     1981 tctaaacccg ctccccctgc aggttgaaaa ccattggcct tgcacatagt acagccaagt
     2041 ctttaatacc caagctgcac taataaattc agattgttgc ggatgggctg caaatgcatc
     2101 ctttataggc ttggcaaaaa aacgctcttt ggggctctct gaaataaatt ctcagctagg
     2161 atacttattc aaacctagca accgcatttc gtgagggatc ttatttgagc ccgtttggcg
     2221 gaacggttaa aaatcacagc cgtatcatgc ttaaacctaa aaaccgcagt tgaactctac
     2281 tacggccgcc taatgcttga atctg
//
LOCUS       DAOQGS010000642         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30755_length_2294_cov_7.540866, whole genome shotgun sequence.
ACCESSION   DAOQGS010000642 DAOQGS010000000
VERSION     DAOQGS010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30755_length_2294_cov_7.540866"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..792)
                     /locus_tag="PKZ52_17235"
     CDS             complement(178..792)
                     /locus_tag="PKZ52_17235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNG61570.1"
                     /translation="MFSIYQLKPAFQNLLRPLVRSCAGRGITANAVTLTALIISLVVG
                     AVLIVYGQAQPGWFLLLPLWMFFRMAFNAVDGMLAREFGQQSALGAYLNELCDVIADA
                     ALYLPFAFIAPFSIWSIGALIWLAALSEMTGALGPLVGAPRAYHGPMGKSDRAFVFGC
                     LGLLVGLGITLPAWCTAVIYIMCGLMGVNMIKRIRAGISVSAKP"
     gene            complement(1487..2065)
                     /locus_tag="PKZ52_17240"
     CDS             complement(1487..2065)
                     /locus_tag="PKZ52_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61571.1"
                     /translation="MTTRTYTWSPIISLHDYLQAPSTPGIYEIGFKKPGYLAPQYGNI
                     GTFGLTYPADFHPMYSGKHQASIRKRLGEHYVGVNSVGNFRSGTKANSFIREYYNSLD
                     FLRSASIPEHLEWTRTGLFFTYLCTSDPAAIEGYYRLNHFEYSWNKKTELSAAQRAAA
                     EGVKDLAGLPSKICHMIFEDGHLVGKGGAKSL"
BASE COUNT          605 a          584 c          531 g          574 t
ORIGIN      
        1 aatcctcatt tactgcgtgt aaactccggt tttttcgcca aatttgcctt gccagtgcgt
       61 cgccgcctac gtatttcaac aagctgttaa gggcgcccac actagctcaa tcgcacggaa
      121 aatacagcct tttttagccg ggcggtcgcg gcatggcctc ggtaaaaatt gtgtaaatta
      181 gggttttgcc gacacgctaa ttcccgcccg aatgcgttta atcatgttaa cgcccatcag
      241 cccgcacatg atatagataa ccgccgtgca ccatgcgggc agagtaatgc caagccccac
      301 caataaaccc aggcagccaa atacaaacgc ccggtcactt tttcccatgg ggccgtgata
      361 ggcgcgcggc gcccccacca aaggccctaa agcccctgtc atttctgaaa gagccgcaag
      421 ccaaattagc gcgcctatgc tccaaataga aaagggcgca ataaacgcaa agggcaaata
      481 aagcgcggcg tcggcaatta catcgcaaag ctcgtttaaa taagctccca gcgccgattg
      541 ctggccaaat tcccgcgcta acatgccgtc cactgcatta aacgccatgc gaaaaaacat
      601 ccacagcggc aatagtaaaa accagccagg ttgagcctgg ccgtaaacaa ttaacaccgc
      661 tcctacaaca agggaaataa tgagtgccgt tagggtaact gcattggctg taatgccgcg
      721 cccggcacag ctgcgcacca acgggcgcaa taaattctga aaggcgggtt tgagttgata
      781 aatactaaac aatctaggct cctgttgttt aacggttccc cgtgggcggg gcgcggcatc
      841 atatagtttt tcaacgctag tgcctataag gctgccgcgg cggccattca aatcaattag
      901 cgccaataac aatttatatt gtcaattcct atcgcgtaaa tgctttttgg atgcagaaaa
      961 tctgcatttt gcgcaaggat ttttcggatg gtttaaacga ttaaaaatga gcgatgtgaa
     1021 tagataactg ctattttgat aggcgaatgc tttcgggggt ataaagaatg actagttggc
     1081 gggaaactgc cgccagcttt gataaatgcc gtgtgcaatt gaaaatacca ggcttgcggc
     1141 gttgcattaa acttaattat tgcttggcag ggcctgcaaa atcatacaag cggattaaca
     1201 attaatctag ggaacctctg aaaaattacc ttactcgccc tggcggcgtt gaatttggca
     1261 aaaaaatgct catttactgc gtgtaaactc ggcaattgct cctgcattgc tctacctcga
     1321 tttcattccc gatgaaattc tacctccccc ttccatgggg tcgtcctgca tccatgcagt
     1381 cgtccgcttt ttttgccaaa ttcgcctagc cagagcttcg ccgcccacat ttttcagagg
     1441 ttcccctagc taaatgccca taggctagct ggctgcttaa aaaggcttag aggcttttgg
     1501 cgccgccttt gccaaccaga tgcccatctt caaatatcat atgacagatc ttggacggca
     1561 agcctgctaa atcttttaca ccttcagccg cagcccgttg tgccgctgat aattcagtct
     1621 ttttattcca gctgtattca aaatgattaa ggcgataata accctcgatt gcagcaggat
     1681 ctgaggtgca taagtaggta aaaaataacc cggtacgagt ccactctagg tgctctggaa
     1741 tactcgcaga gcgcagaaaa tccagagaat tgtagtattc tcgaataaaa ctattggctt
     1801 tggtgccaga gcggaaattg ccgaccgagt tgacgcccac atagtgttcg cccaatcgct
     1861 tgcggataga ggcctgatgc ttgcccgaat acattgggtg aaaatcggcg ggataggtaa
     1921 gaccaaatgt gcctatgttg ccatattgag gtgctaagta gccgggcttt ttaaagccaa
     1981 tttcatagat gccaggcgtg ctgggtgctt gtaaataatc gtgcaaactt atgatgggcg
     2041 accaagtgta ggtgcgagtt gtcataatag atcctctaac agcgtgttga aatactaccc
     2101 tgcaagccct ggcggcgtta aatttagctc ggcgcacccg ctaaaaaatc ctccttgtac
     2161 ggcgtgtaaa ctccggcttt tgtagaggcg ccgagcccaa tgtgccttgc tgtcgccgcc
     2221 aatatttttc aacgcgctgc tattaacccg gcggctaaat agatcgtcct gatctattta
     2281 gccttcgctg ccct
//
LOCUS       DAOQGS010000643         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30779_length_2293_cov_4.227882, whole genome shotgun sequence.
ACCESSION   DAOQGS010000643 DAOQGS010000000
VERSION     DAOQGS010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30779_length_2293_cov_4.227882"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..>2293)
                     /locus_tag="PKZ52_17245"
     CDS             complement(201..>2293)
                     /locus_tag="PKZ52_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG61572.1"
                     /translation="GGPVIDDLIAWRGNLSVSKGKGDVTNAYNTDQTYTNKDRVSGKV
                     QFLITPSETLSARLEADIQPRAGETTNGRTINTPTPLKYADGKDNTAIDNAKRLSRRW
                     FSQNPNYTVDGNFYYGAGGKNVNNDSARPLNSGSNGATAEVNWRLEDYTLTSITAYKD
                     YHFNAVNDEGTPFDVNRNSGGFFNDYSQRSQELRLSSKVGGFVDYQTGLFFLDVKNYS
                     LYQRVWGNDAGAWFANNAQYTSLDKDGNGRYLLQNSLAGLSMAWNSPAGLQQIHNTSS
                     AIFGQANWHFTPKFTLTTGLRVTQEDRNNTASTYIRDQGNSPELNPDLVEGLNGNSVN
                     LGGFASTDKGVFGKVDKLTGVIVDNNTPAQRSAADKVAQKYFNKTPTATPGEAYNSLT
                     PDQKQQVAHAKTLRQGQIGVVFNSTELQPFKKTQPSFVLSPSYKISEDVTVYSSYQHG
                     EKAGISQATNGQSNLVKEEKTDAIELGIKTNLLDNTLVFNAALFRMDITDYQQTSSVL
                     DTYTTTTRNDNTTYYTNATANIPKVRSQGLEIDTLYNGFRNLTLRFAGAYTDAYYVEF
                     TNSPQPVENNNQALKFRDVSGETLLGASKITFNIGVDYRVPVLSNLEFHTSSNILYAS
                     EFNSDNALSSYGKIPAATTIDFAVGLGTAKQTFDANLVVKNLLDDDTHRTQTWNSYTP
                     AVGRWAGVTFSGKF"
BASE COUNT          557 a          499 c          562 g          675 t
ORIGIN      
        1 aataaaaccg cggcgagaac acagcccatg cacataaaga tcaaataatt ttaaaacttc
       61 gggatgaaaa tcattggcgg ttttacgtgg cataagcaat tgtcctgttt gtggtgggtg
      121 gagaaacagg cagcctatct attagttaaa caggccacct gctcgattaa gggcactaac
      181 caaatgggat ttaaatggaa ttaaaattta ccgctaaagg ttaccccagc ccatcggccc
      241 accgctggtg tgtagctatt ccaggtttgt gtgcggtggg tgtcatcgtc caacagattt
      301 ttcactacca gattggcatc aaacgtctgc ttggcggttc ctaggcccac tgcaaaatca
      361 attgtggtag cagcggggat tttgccatag cttgataagg cgttatctga gttaaattca
      421 ctggcgtaaa ggatgttcga gctggtgtgg aactccagat tgctgagtac aggcactcga
      481 tagtcaacgc caatattaaa ggtaattttt gatgcaccta gcagggtttc gccactaaca
      541 tcgcggaatt ttaatgcctg gttattgttc tctactggtt gtggcgaatt ggtgaattcc
      601 acatagtagg catccgtata agcaccggca aagcgcaggg tcaaatttct aaacccatta
      661 tatagagtgt cgatctctaa accttgcgag cgcaccttgg ggatattagc cgtggcattg
      721 gtgtaataag tggtgttatc gtttcgagtt gtggtggtat aggtgtcaag tacgctactg
      781 gtttgttgat aatccgtaat gtccatgcgg aataaagcgg cgttaaaaac cagggtgtta
      841 tctagcagat tggttttaat gcccagctct atagcatcgg ttttttcttc cttcactaaa
      901 ttggactggc cattagtcgc ctgagaaata ccggcttttt caccgtgctg gtacgaacta
      961 taaaccgtta cgtcttcgga tattttatag ctggggctga gcacaaatga gggctgggtt
     1021 ttcttgaacg gttgtaattc tgttgagtta aataccacac caatctgccc ctggcgcagg
     1081 gttttggcat gggcaacctg ttgcttttgg tctggtgtta aactgttata agcttcccct
     1141 ggcgtggcag taggtgtctt attgaagtat ttttgcgcaa ctttatctgc agctgaacgc
     1201 tgagccggtg tattgttatc gactatgacg cctgtcagtt tatccacctt gccaaacacg
     1261 cctttgtctg tcgaggcaaa cccacctaaa ttgacggaat taccattgag cccttcgact
     1321 aggtcgggat taagttctgg tgaattgccc tgatcgcgga tgtaggtaga cgccgtattg
     1381 ttgcgatctt cctgagtaac acgcaggcca gtagtaagag tgaacttggg cgtaaaatgc
     1441 cagttagctt ggccgaagat tgcactgctg gtgttgtgaa tttgttgtaa acctgccggg
     1501 ctattccaag ccattgatag gcccgctaac gaattttgca gcaaatatcg gccattacca
     1561 tctttatcca gagaggtata ttgagcgttg ttagcaaacc acgctccggc atcattcccc
     1621 catacacgct gataaagcga atagtttttg acatcaagga aaaataaacc cgtttgatag
     1681 tcaacaaaac cgcccacttt tgaggagagg cgtaattctt ggctgcgctg actataatcg
     1741 ttaaaaaatc caccggagtt gcggttgaca tcaaacggag ttccctcatc gtttaccgca
     1801 ttaaagtggt aatccttata ggctgttatt gaggtaagtg tgtagtcttc aaggcgccag
     1861 tttacttctg cggtggcacc gttgctgccg gaattaagtg gccgtgcact atcattattt
     1921 acattttttc cgccggcccc gtaataaaaa ttgccatcaa ctgtgtaatt ggggttttgc
     1981 gaaaaccacc ggcggcttaa acgtttggcg ttgtcaatgg cggtgttatc tttaccatcc
     2041 gcatatttaa gtggcgttgg tgtattaatc gtgcgtccat tagttgtttc ccccgcccgc
     2101 ggctgtatat ccgcttccaa ccgggcgctc agtgtttcgc tgggcgtaat taaaaactgc
     2161 accttgccgg aaacgcgatc tttattggtg taggtttggt cggtgttgta ggcgttggtt
     2221 acatcgcctt ttcctttact tacacttaaa ttaccgcgcc aggcaattaa atcgtcaatt
     2281 actgggccac cgg
//
LOCUS       DAOQGS010000644         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30782_length_2293_cov_3.756032, whole genome shotgun sequence.
ACCESSION   DAOQGS010000644 DAOQGS010000000
VERSION     DAOQGS010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30782_length_2293_cov_3.756032"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PKZ52_17250"
     CDS             <1..1234
                     /locus_tag="PKZ52_17250"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNG61573.1"
                     /translation="RSDGGLLATLAEMAFAGRCGLTIDFTQLPNAEPLALLFNEELGA
                     VIQVAQEHLDAVEASFAAAGVHHVYNIGRAQVEQTITLGKDRDVWIKASRAQWQTLWA
                     ETSYRMQALRDNADCALQEHQRIGQDDPGLSAKLVYNPADDIAAPYINTGVRPQVAIL
                     REQGVNSQLEMAAAFERAGFAAVDVHMSDILSGRVNLQGFKGLAGCGGFSYGDVLGAG
                     EGWAKSILFNSRAREEFETFFHRNDTFALGVCNGCQMMSNLKSLIPGAGHWPRFVRNL
                     SEQFEARVALVKVEQTPSVLFAGMAGSHLPVAVSHGEGRAEFADGQAFKKCNESGLVA
                     LRFVDHQINTTEVYPYNPNGSPAGITSLTTQDGRVTILMPHPERVFRTITNSWQPPGW
                     QADGGWLRLFRNARVFVK"
     gene            1501..>2293
                     /locus_tag="PKZ52_17255"
     CDS             1501..>2293
                     /locus_tag="PKZ52_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNG61574.1"
                     /translation="MLRLKPLNSLCAALTLIISSLAQAEQLPYQNPALPVDQRVADLI
                     GRMTLDEKIAQLQTRWWEMVPLLDANGNFSPQEAQKIMPLGIGELARPGQVKNNYAPN
                     RTPQQTAQYTNSIQRWLKEHTRLGIPAIFHDEALHGLSGLNATSFPQAIALASSWQPP
                     LLEEIYTQVAKEVRCRGSQRVLAPVLDVARDPRWGRIEETLGEDPYLVASLGVAAVKG
                     FQGEGATPDNQHVITTLKHLAGHGEPSGGLNTAPANLGERLLREVF"
BASE COUNT          466 a          685 c          673 g          469 t
ORIGIN      
        1 ccgcagtgac ggtggtttgc tggccaccct agcggaaatg gcctttgccg gtcgctgcgg
       61 cttaaccatc gacttcaccc agttgcccaa tgccgagcct ttggcgcttt tgttcaacga
      121 agaattgggt gcggtaattc aggtggccca ggagcacctg gacgcggtgg aggcgagttt
      181 cgccgctgct ggcgtgcacc atgtgtacaa catcggccgc gcccaggtgg agcaaaccat
      241 cactctgggt aaagaccgcg atgtgtggat caaagccagc cgcgcccagt ggcaaaccct
      301 ttgggcggaa accagctacc gtatgcaggc cctgcgcgac aacgccgact gtgccctgca
      361 ggagcatcag cgcatcggcc aggacgaccc cggcctcagc gccaaactcg tctataaccc
      421 cgccgacgac atagccgcgc cttacatcaa taccggcgtg cggccccagg tcgccatcct
      481 gcgcgagcag ggggtgaact cccagctgga aatggccgcc gcctttgagc gcgcaggctt
      541 tgctgcggtg gatgtgcaca tgagcgatat tctcagcggg cgggtgaacc tgcagggctt
      601 taagggcctg gccggttgcg gcggcttctc ctatggcgat gtgttggggg ccggggaggg
      661 ctgggctaag tccatcctgt ttaatagccg ggcgcgggag gagtttgaaa ccttcttcca
      721 ccgcaacgac accttcgccc tcggggtgtg taacggctgc cagatgatgt cgaatttaaa
      781 atcgctaatt ccaggcgccg gccactggcc gcgctttgtg cgcaacctgt ccgagcaatt
      841 tgaggcgcgg gtggcgctgg tcaaagtgga gcagacccca tcggtgttgt tcgccggtat
      901 ggcgggctcc cacctgccgg tggcggtctc ccacggcgag gggcgggccg aatttgccga
      961 tggccaggcg tttaaaaaat gcaacgagtc tggcctggtg gccctgcgtt ttgtcgatca
     1021 tcagatcaac accacggaag tctatcccta caaccccaac ggttcgccgg ccggcatcac
     1081 cagcctcacc acccaggatg gccgcgtgac tattttgatg ccccacccag agcgggtttt
     1141 ccgcaccatc accaactcct ggcagccacc cggctggcag gcggacggcg gctggttgcg
     1201 cctgtttaga aacgcccgcg tatttgttaa ataattttta acaccttagg taacaggcaa
     1261 ccacctgggc ggtttaactc ggcagttaaa ttggccccgg tgggttattt atgggcgcct
     1321 gtacatgcgc cctgatgcgg gttactgcgg cggggtgaat tattctgtat gcctataaat
     1381 cacatataaa aatgcattgc tgcgcgggtt aggaattttt ctatttaggg tcaatacttg
     1441 tggctatcgc cgcgcctggc gcctctttta tgcgttccct ggaacaaagg acaattacct
     1501 atgcttcggc ttaaaccgct caatagcctg tgtgccgccc ttactctcat catcagcagc
     1561 cttgcccagg ctgagcagct gccctatcaa aatccggcgc tgccggtaga tcaacgggtg
     1621 gctgacctga tcgggcggat gaccctggat gaaaaaatcg cccaactgca aacccgctgg
     1681 tgggaaatgg ttcctctgct ggatgccaat ggcaattttt ccccccagga agcgcagaaa
     1741 atcatgcccc tagggatcgg cgaattggcc cgcccagggc aggttaaaaa caactacgcg
     1801 cccaatagaa cgccccaaca aaccgcccaa tacaccaata gcatccagcg ctggttaaaa
     1861 gaacataccc gcctaggcat tccggctatt ttccacgatg aggccctgca cggtctgtcg
     1921 gggcttaacg ccaccagttt cccccaggcc atagccctcg ccagcagctg gcagccgccg
     1981 ctcttggaag aaatttatac ccaggtagcc aaagaggtgc gctgccgagg ttcccagcgg
     2041 gtgttggccc ccgtgttgga tgtggcgcgg gacccgcgct ggggccgtat tgaagaaacg
     2101 cttggggaag atccttacct ggttgcaagc ctaggcgttg cagccgtgaa ggggtttcag
     2161 ggggagggcg caactcccga taaccaacac gtaattacca cgcttaaaca tctggccggc
     2221 cacggtgagc ccagcggcgg cctcaacacg gcgcctgcca acctaggtga gcgcctattg
     2281 cgggaagtgt ttt
//
LOCUS       DAOQGS010000645         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30833_length_2291_cov_2.970930, whole genome shotgun sequence.
ACCESSION   DAOQGS010000645 DAOQGS010000000
VERSION     DAOQGS010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30833_length_2291_cov_2.970930"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2291
                     /locus_tag="PKZ52_17260"
     CDS             <1..>2291
                     /locus_tag="PKZ52_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNG61575.1"
                     /translation="QVDKEERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIDGD
                     DRADLKDALSHIDVPQGMGVIIRTAGVGRTAEELQRDLSRQVQLWESIKASAESVPAP
                     HFLFEESDVIIRAIRDYLRPDIVDVYLDTRESFEKAQAYVQRIMPEYLNRIKLYQESL
                     PLFNRYQIEGQIETAFEREVKLPSGGAIVIDITEALVSIDINSSRATKGGDIEETALA
                     TNLEAADEIARQLRLRDIGGLVVIDFIDMQAPRNQRDVVNRMRDALAIDRARVQIGTI
                     SRFGLLEMSRQRLRPSLEETTSKVCPRCHGQGTIRDTKSLSLSILRIIEEEAKKERSA
                     EIRAVVPVPVGTFLLNEKRTAISQIEARHNARVVILPNADMVTPHYQVTRLRDDEIDN
                     TELSYNIKLINEDAIEEAQDAKAALPSTAKAAISQITPLGAPVEIPTSKSEPAATAPT
                     EAGIVGKLMSFFGNLFKSEAPAKEEVSTQSKPQTGRNRHEGRQRHRRDHRRDRNERSD
                     RKEEITDNRSAESSDRPAQKPRHERADNRVRDEKPSRSEAVETTKNADRSPNDRSRNR
                     RGRAQEVRALEPAAEANAPVADNKLEQEDRPQRRPNNRRPKPHERQRGPRTEEVAAQE
                     EVAADQPIDLTELEEQLSAAIAENQKARQETPVNPFIDTASLQTEPAAAITAEAPAAD
                     PIPGPIESTPEIAPQVPATSEQASPVVALETADSVLAAAETPEEDQRERHQPRRGAQN
                     DAAAPQRSIECAGVETGQERHRRV"
BASE COUNT          578 a          735 c          607 g          371 t
ORIGIN      
        1 caggtggaca aagaagagcg cggcaacaag ggcgcagccc ttaccacatt tatcagccta
       61 gccggccgct acttagtatt aatgcccaac aacccccgcg caggcggcat ctctcgccgt
      121 attgacgggg atgatcgggc cgaccttaaa gatgccctca gccacattga tgttccccag
      181 ggcatgggcg taatcatccg taccgcaggc gttggccgca ccgcggaaga actgcagcgg
      241 gatttgagcc gccaagtaca gctgtgggaa tccatcaaag cctccgccga atcagtgccc
      301 gccccgcact ttctgtttga agaaagcgac gtcatcatcc gcgctattcg cgattatttg
      361 cgcccggaca tagtggatgt gtacctggat acacgggaat ccttcgaaaa ggcccaggcc
      421 tatgttcagc gcatcatgcc tgaatacctt aaccgcatta agctgtacca agagagcttg
      481 ccgcttttta accgctatca aattgaaggc cagattgaaa ccgcctttga gcgagaagtg
      541 aaactgccct cgggcggcgc cattgttatt gatatcaccg aagcactggt cagtatcgat
      601 atcaactcct cccgcgccac taaaggcggc gatattgaag agaccgccct tgccaccaac
      661 cttgaagccg ctgatgagat agcccgccag ctgcgcttgc gggacatagg cggcctggtg
      721 gtaatcgact ttatcgacat gcaggcgccg cgcaatcagc gcgacgtagt caatcgtatg
      781 cgcgatgccc tagccattga ccgcgcccgt gtgcaaatag gcaccatctc gcgatttggt
      841 ttgctggaga tgtcccgcca gcgcctgcgc ccatcccttg aggaaaccac ttccaaggtc
      901 tgcccccgct gccacggcca gggcaccatt cgcgatacca aatccctctc cctgtcgatt
      961 ctgcgcatca tcgaagaaga ggcgaagaaa gagcgcagcg ctgaaatccg cgccgtggta
     1021 cctgtaccgg tcggaacctt cctgctcaat gaaaagcgta ccgccatcag tcaaattgag
     1081 gcccgccata acgcccgcgt ggtgatcctg cccaacgccg atatggtaac gccacactat
     1141 caagtcacgc gcctgcggga cgatgagatc gacaataccg agctgagcta caacatcaaa
     1201 ctcatcaatg aagacgccat tgaagaagct caggacgcca aagctgcctt gccttctacc
     1261 gccaaagcgg ccatcagcca gatcacaccg ctgggcgccc cagtggaaat acccaccagc
     1321 aaatccgagc ccgccgccac agcgcctaca gaagcgggca tagtgggtaa gttgatgagt
     1381 ttcttcggca acctgtttaa atcggaagca ccagctaaag aagaagtcag cacgcagagc
     1441 aaaccccaaa cagggcgcaa tcgtcacgaa ggccgccagc gccaccgccg ggatcaccgc
     1501 cgggaccgca atgagcgctc ggaccgcaaa gaagaaatta ccgacaaccg ctcagctgag
     1561 agcagtgatc gccccgctca aaaacctcgc catgagcggg ccgataaccg cgtgcgcgat
     1621 gaaaaaccct cccgttccga agcagtagaa accactaaaa atgccgatcg atcgcctaac
     1681 gatcgatccc gcaaccgccg cggtcgggca caggaagtgc gcgctttaga acccgccgca
     1741 gaggccaatg cacctgttgc cgacaacaag ctggagcaag aggatcgccc ccagcgccgc
     1801 cccaacaatc gtcgccctaa gccccacgag cgccaacgag gtccgcgaac agaggaggta
     1861 gcggcccaag aagaggtggc tgccgatcaa ccgattgatt taacggaatt agaagaacag
     1921 ctcagtgcag caattgccga gaaccaaaaa gcccggcaag agacgcctgt aaaccctttc
     1981 atcgacaccg catcactgca aacagaaccc gctgcggcaa ttactgcaga ggctcctgcg
     2041 gccgacccca tacctgggcc gatcgaatcc acacctgaaa tagcccccca agtaccggcg
     2101 actagcgaac aggcgagccc tgtcgttgca ctggaaactg cagattctgt attggcggct
     2161 gccgagacgc ccgaagagga ccagcgagag cgccaccagc cccgccgcgg cgctcagaac
     2221 gacgcggcgg cgccccaacg ctctatcgag tgcgccggcg tagagacggg ccaggagcgc
     2281 catcgccggg t
//
LOCUS       DAOQGS010000646         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30842_length_2290_cov_4.512304, whole genome shotgun sequence.
ACCESSION   DAOQGS010000646 DAOQGS010000000
VERSION     DAOQGS010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30842_length_2290_cov_4.512304"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKZ52_17265"
     CDS             complement(<1..291)
                     /locus_tag="PKZ52_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNG61576.1"
                     /translation="MRELLLITVSGQDRPAVTSAVTGILAQHPCRILDIGQAVIHDHL
                     SLGILAEFAQHSAASEAVKNVLYAMHERNLQVRFTPITEAAYKEWVAQQGKER"
     gene            374..1558
                     /locus_tag="PKZ52_17270"
     CDS             374..1558
                     /locus_tag="PKZ52_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61577.1"
                     /translation="MYNARLFHPASPLGVRFREARDTIIFRLHTANIPRLFYLAIGLL
                     IGLLSCATVLRAVERAQEQVLLADYGQSLANLAAKRAVDATLNHDLVSLQVVLQDVMD
                     NPRTLLASIHDVENNLLVQAGSTQALTHAAALRSFSAPIPLQDSIAGFVTVNIESQMP
                     EIKALYLSFAAVCSLLVIVALLSLLNRSGPPFSVTHTPREKPAAKDTAADEPTPGTET
                     PPVFTAYLRWQWHNLATLRQQISAERLEQLFNRLELILVEAVKITGTQCQTRRKQGRQ
                     NGEYWLTYSSGESILVAYYQLMVCAHLIHHLVREEKLKLVISSTLTSSEEIPPEHSAE
                     HFPGQIVCLLPEGIDEEWDNWIYFNEVPAGLQLMGFVPPLDDDIAELLTGLRAAIDAR
                     TT"
BASE COUNT          525 a          648 c          576 g          541 t
ORIGIN      
        1 gcgctcttta ccctgctggg ccacccactc tttgtaggca gcctccgtaa tgggggtaaa
       61 gcgcacctgt aaattgcgct cgtgcatggc gtagagcacg ttttttaccg cttcggaggc
      121 cgccgaatgt tgggcaaatt ccgccagaat gcctaggctt aggtgatcgt gtatcaccgc
      181 ttggcctata tcgagaatac ggcaggggtg ttgcgccaaa atacccgtta cggcagaggt
      241 caccgccggt cggtcttggc ctgagacagt aattaacaat aattcacgca cgctaaatca
      301 acctggcaaa aggggcggca ttatagggga tttatcagcc cgtgcggcta actcacctat
      361 ccgtgtgtgc gctgtgtaca atgcgcggct ttttcacccg gccagcccct tgggtgtacg
      421 ttttagagaa gcgagggaca ccattatttt tcgcctgcac accgccaata ttccccgttt
      481 gttttacctt gcgattggcc ttttgatcgg gttgctcagc tgcgccaccg tcttgcgggc
      541 ggttgagcgc gcccaagaac aggtcttact cgccgactac ggccaatccc tcgctaacct
      601 cgccgccaaa cgcgcggtgg atgccaccct caatcacgat ttagtctccc tgcaggttgt
      661 gctgcaggat gtaatggaca acccgcgcac cctgctggca agcattcacg atgtggaaaa
      721 taacctctta gtacaggcgg gctccaccca ggccctcacc cacgctgccg ccctgcgcag
      781 ctttagcgcg ccgatcccgt tgcaagactc cattgcaggt tttgtgacgg tgaatatcga
      841 aagccaaatg ccggagatta aagccctgta tttaagtttt gcggcggtgt gcagcctgct
      901 ggtgatagtg gcgctgctaa gcctgctcaa ccgcagcggg ccgccctttt ccgtaacaca
      961 tacgcccagg gaaaaacccg ccgccaagga taccgccgct gatgagccaa cccctggcac
     1021 cgaaacgccg ccggttttta ccgcctattt gcgctggcag tggcataacc tcgccaccct
     1081 gcgccagcag atcagtgccg agcggttgga acaattattt aaccggctgg aattaatttt
     1141 ggtggaagcg gtaaaaatca ctggcaccca atgccaaacc cgccgcaagc aaggccgtca
     1201 aaatggtgaa tattggctca cctacagcag cggcgaatct attttggtgg cctattacca
     1261 attaatggtg tgcgcccatt taattcatca cttggtgcgt gaagaaaaac ttaaattggt
     1321 tattagcagc acccttacca gcagcgagga aataccccca gagcacagcg ctgagcattt
     1381 ccccggtcaa attgtttgcc ttttgcccga gggcatagat gaagagtggg acaattggat
     1441 ttatttcaac gaagtgcccg caggcctgca attaatgggt tttgtgccgc cgctagacga
     1501 cgacattgca gaattgttaa caggtttacg cgcggcaatt gatgcacgta caacttaaaa
     1561 ccaccagcgc ccagccgtat attctaccct tggcacagca gccgaagccg cagccaagct
     1621 gctgacacca attgggaaaa gcataattaa gtgcaagtat ttagcacagc ttgttacccc
     1681 cgccacacgg gcggcaaatt gagaggaatt taactgggtt cagggtgtgg cagtatcaaa
     1741 agctctcagc actaaagcac tcgccctatg gtttttagtc ggccagaatc gtattcagcc
     1801 gcatgaaatt aagaacgcac tgactaagca ggcatgttta gcagaacagc tcatgttcgc
     1861 aaaaaacttt ccctctaacc aacgcccaca cctaaaaaca cctcaggcaa tgtgcgactg
     1921 ctgtgtgcac ttgtgcaaac tctgcggcaa gccggttgtt aacccctgcg catttcgcag
     1981 ttgtagccgt gttttgcggc gttgcgcgca gcattaagca ttcgcgccgt gttagcatcg
     2041 agtcgttttg aatacatgtc tcggcactgt gtacgccgtg catttattga tatttatcgc
     2101 attattttga atttcaccga ggcaacgcct cgccacttaa gtgcatcccc aggaggattt
     2161 catgaaggca ttggtactgc gccagcattc attcgctcgc ttttttccgg ccgctgtttt
     2221 ggccactagc ctagttgcag gcctgaccgc ttgcggcgcc gaccccgctg gcaacacccc
     2281 cttaaccggt
//
LOCUS       DAOQGS010000647         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30973_length_2284_cov_4.925527, whole genome shotgun sequence.
ACCESSION   DAOQGS010000647 DAOQGS010000000
VERSION     DAOQGS010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30973_length_2284_cov_4.925527"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PKZ52_17275"
     CDS             <1..1624
                     /locus_tag="PKZ52_17275"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNG61578.1"
                     /translation="GHFSIWRGGFTQFYEEPHTWTGLVVGWILFFVFVMGNFGRFDTE
                     IDQWMRPELPIETASLNQSIAAAQQRLQAKAPQAEIWTIWPATNRDYPNLRIRWETAP
                     NAEGARKRVDETLNANTAEPVVARDTGGGQLLYKMHYALHYIPRSVSDWLIGICSFFM
                     LVAIVSGVITHKKIFADFFTFRPSKGQRSWMDLHVVLATLTLPFFLMITYSGLIFFVF
                     TLFPQPLNTAYGTAANARETFNNELNARAHIQERSHQPAQLVNLTSLIETVESRWGKN
                     QIRLIEVNNPNDSTARIRVMHLLDSSSRSSSEITFDGVSGTVLEENKGETTAARGFYD
                     HVLGLHRGLFGSFSLRWLYFFSGVIGAIIIASGLVLWIVKRRPKQEKQQQPDFGFRLV
                     EGLNVGTIAGLPIAIAAYFWANRLLPLDLSSRGDWEVHSLFIVWLLALLYPLIRPIPR
                     AWIEQFWLAGVGFLLLPLINAATTERHFFNSIIKGDSVFLAIDIAALITGLLFISGAV
                     TLTRRQKTQLAVQDRPARSTKTLNTLSSAEAT"
     gene            1626..1943
                     /locus_tag="PKZ52_17280"
     CDS             1626..1943
                     /locus_tag="PKZ52_17280"
                     /inference="COORDINATES: protein motif:HMM:NF023232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HNG61579.1"
                     /translation="MALSFFIAYFGFALLSLAKNRHFQQVWPHKKLTITLERELRISG
                     WLLLLISTTHLIQAPKISIGLTDVIGIWTFAALIVVLQLTYLPRSVAGIGIITQLLPR
                     SKN"
BASE COUNT          570 a          581 c          511 g          622 t
ORIGIN      
        1 aggccatttt tccatctggc gcgggggttt tacacaattc tacgaagagc cacatacttg
       61 gacaggttta gtggtgggct ggatactatt ttttgtgttt gtcatgggca attttggccg
      121 cttcgataca gaaatagacc agtggatgcg gcctgaattg ccaattgaaa ccgccagcct
      181 caatcaatcc attgcagcgg cgcagcagcg cctgcaagca aaagcgccgc aagcagagat
      241 ctggactatt tggcccgcca ccaatcgcga ttatcccaat ttgcgtatcc gctgggaaac
      301 cgccccgaat gcggaaggcg cccgcaaacg cgtggatgaa acactcaacg ctaacacggc
      361 agaacccgtg gtggcgcggg atactggcgg cgggcaattg ttatataaaa tgcactacgc
      421 acttcactat ataccccgca gtgttagcga ctggttaatt ggtatttgca gtttttttat
      481 gttggtggca attgtcagcg gcgtcatcac tcataaaaaa atattcgcgg atttttttac
      541 ctttcgccca tccaaaggcc agcgctcttg gatggatctg cacgtggtgc ttgccaccct
      601 caccctgcca tttttcctaa tgattaccta cagcggttta atcttttttg tcttcacgct
      661 attcccccaa ccgctcaaca ccgcctatgg aacagccgcc aatgctcgcg agacatttaa
      721 taacgaactt aatgcgcgcg ctcacattca agagcgcagc caccaaccgg cccaattagt
      781 gaatctcacc agcttaattg agacggtaga atcgcgttgg ggtaagaatc aaatccgttt
      841 aattgaagtt aacaacccca atgacagcac tgcccgcatt agggtcatgc acctgcttga
      901 ctccagctcc cgcagcagct cggaaataac cttcgatggc gtaagcggta cagtactgga
      961 agaaaataag ggtgaaacta ctgctgcacg gggtttttat gatcatgttt taggcctgca
     1021 ccggggcctg ttcggcagct tcagtttgcg ctggttatat tttttctccg gggtaattgg
     1081 tgccattatt atagcctcag gccttgtatt atggatcgta aaacgccgcc cgaagcaaga
     1141 aaaacaacaa cagcccgatt ttggctttcg cttagtggaa ggtttaaatg tgggcactat
     1201 tgcgggcttg ccaattgcta tagcagcgta tttttgggcc aaccgcctgc tgccgctgga
     1261 tttatccagc cgcggtgact gggaagtaca cagcctattt attgtttggt tactcgctct
     1321 gctctaccca cttattcgcc ccattccccg cgcctggata gaacaatttt ggctggcagg
     1381 tgtgggcttt ttattattgc cgctcatcaa tgccgccacc accgagcgcc attttttcaa
     1441 cagtattatt aagggcgaca gcgtcttttt agctattgat attgccgctt tgattacagg
     1501 cttattattt ataagtggcg ctgtaacact tacacgccga caaaaaaccc aactggctgt
     1561 acaggatcgc ccggcacgct ccactaaaac attaaatacg ctcagttccg ccgaggcgac
     1621 ctaaaatggc cctaagtttt tttatcgctt attttggttt tgctctgtta agccttgcta
     1681 aaaatcggca cttccaacag gtgtggcccc ataaaaagct gaccattacc ctggagcgag
     1741 aacttagaat atccggttgg ctgctgttgt tgatcagtac cactcattta atacaagcgc
     1801 ctaaaatcag catcggttta accgacgtta ttggcatctg gacatttgcc gctctgatag
     1861 tagtgctgca gttaacctac ctaccccgtt cggttgccgg tattgggatt atcacacagc
     1921 tattacctcg ttcaaaaaat tagtgtactc acaatggtca attatgttta gggtatataa
     1981 aggttcataa aagtggtgat gaggccaagg aaggtaaaag gacttattga gaaaatcgca
     2041 ccgctttcct caaatataac tctggcaagt attccgccgt tacttcttaa aatcggcaaa
     2101 ggttggggct atttttccca cgcggctaat tcagccatcc acaagaatta aacacacttg
     2161 ctctacccac ctgttgaaat accatcttgc cagagcgtcg ccacttacga atttcaacaa
     2221 gctgctaatg ctctgcgctt cacttcttcg catagccggc taatattaac ccggcggcat
     2281 ttta
//
LOCUS       DAOQGS010000648         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_30974_length_2284_cov_4.656348, whole genome shotgun sequence.
ACCESSION   DAOQGS010000648 DAOQGS010000000
VERSION     DAOQGS010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30974_length_2284_cov_4.656348"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PKZ52_17285"
     CDS             complement(<1..1857)
                     /locus_tag="PKZ52_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNG61580.1"
                     /translation="MTFTYLQVALICFAYLAVVFSLAYSTDRGWVSDRLVTHPITYIC
                     SLGIFASAWSFYGVVDLARQFGYGALAYYLGTGVLFLFGGITLKPLIELARRFQITSM
                     ADLLVFRYHSHAAGGVVTLIMLLAMAPLLTLQIQAVADTVHLLTWQHNKNNQLRWFGF
                     TSREAIALVYCLVIALFAMMFGSNREHHKGLVTAMAFESLIKLFGLLAVGGYVLVRIF
                     SGMDGLDWWLNANPEMLQALQRPVYQSSSHTLLLVFLATAISMPHIFHMTVVENPSKM
                     AGAQITWAFPLFLLLMALPIFPILWGGVALELPIAAEYFPLAVPLATGSTSFAIIAFV
                     GGLSAATGAMVGIALALSTMILNHWILPATTLRTKRDLYRQIMWLRRILILVVLLFGF
                     GSYLVLDNRYSLTELALPAFILTLQLLPGVIGVTHWSRGNSLGFIAGLLIGTAIWLVW
                     LMIPMILGVHSISLPLMERPIRLGASEWDFVTLWSIGLNTLVFISVSLLTKQTDEERY
                     SAEICADDELSHPIRLSLDVRSPDEIIERLSSRIGASTARNEVDRALQRLALPPNESR
                     PYALRRLRDEIEANLSGLMGISMASEILDRLVPYRLPETSVATDINLIESRLT"
BASE COUNT          593 a          583 c          695 g          413 t
ORIGIN      
        1 ggtgaggcgg ctttcaatta aattaatatc ggtggccacg ctggtttccg gcagccggta
       61 gggcaccagg cggtcgagaa tttcgctggc catggaaatc cccatcaggc ccgacaggtt
      121 ggcttcaatt tcatcgcgca gacggcgcag ggcgtaggga cggctttcat tggggggcag
      181 ggcgaggcgc tgcagggcgc ggtccacttc attgcgggcg gtgctggcgc cgatgcggct
      241 gcttaggcgt tcgataattt catccggcga gcgcacatcc aggcttaagc gaatagggtg
      301 ggagagttca tcgtcggcgc aaatctcggc gctgtagcgc tcttcgtcgg tttgtttggt
      361 gagcagggat accgatataa ataccagggt attaaggccg atgctccaca gggtgacaaa
      421 atcccattcg ctggcaccta agcgaatggg ccgctccatc aggggtaggc tgatggagtg
      481 cacacccaaa atcatcggaa tcatcagcca caccagccaa atagcggtgc cgattaaaag
      541 gccggcaata aaccccaggc tgttgccccg ggaccaatgg gtaacaccaa tcacgccggg
      601 cagcagttga agggtcagaa taaaggcggg cagggcaagt tcggtgaggc tgtagcggtt
      661 gtcgagcact agataactgc caaagccgaa cagcaatacc accaggatga gaatccgccg
      721 cagccacata atctgccggt ataggtcccg cttggtgcgc agcgtggtgg cgggcaagat
      781 ccagtggttc aaaatcatgg tagacagggc gagggctatc cccaccatgg cgccagtggc
      841 ggcggagagg ccgcccacaa aggcaataat cgcaaacgag gtggagccgg tggccagggg
      901 caccgccagg gggaaatatt cagccgctat gggcagctcc agggcgacac cgccccacaa
      961 aatgggaaaa atcggcaggg ccatgagcag taaaaacagc gggaaggccc aggtaatctg
     1021 cgccccggcc attttgctgg ggttctccac caccgtcata tgaaagatat gcggcatgga
     1081 aatcgcggtg gctaaaaaca ccagcaataa ggtgtgtgag gaggattgat agaccggcct
     1141 ttgcagggct tgcagcattt ccgggttggc gttgagccac cagtccagcc cgtccatacc
     1201 gctaaaaata cgcaccagca cataaccgcc caccgccagt aggccaaata gtttgatcag
     1261 ggattcaaac gccatggcgg tgaccaggcc tttgtggtgc tcccggttgg agccgaacat
     1321 catggcaaac agggcgataa ccaagcagta caccagggca atcgcctcgc gggaggtaaa
     1381 cccaaaccag cgcagttgat tatttttatt gtgttgccag gtgagcagat gcacggtgtc
     1441 ggccaccgct tgaatctgca gggtgagcag cggcgccatg gccagcagca taatcagcgt
     1501 aaccacgccg cccgcggcgt gggagtggta gcgaaatacc aataaatccg ccatggaggt
     1561 aatttgaaag cggcgggcca gctctataag tggtttaagg gtgatgccgc caaataaaaa
     1621 caacaccccg gtgcccaggt aataggccag ggcgccgtag ccaaattggc gagctaaatc
     1681 cactaccccg taaaacgacc aggcgctggc aaaaatacct aaagagcaaa tataggtgat
     1741 tgggtgagtg actaagcggt cggaaaccca gccgcgatcg gtgctgtagg cgaggctaaa
     1801 caccaccgcc agataggcaa aacaaatgag ggcaacttgc aggtaggtaa acgtcatggg
     1861 gattttttat ggcgttgaat gaggtaggcc gccaacacaa ccaaagccca cagcaaaaag
     1921 ggtttgtacc aaataggctg cgggttaatc gcccaactaa taatgctagg cgagagcata
     1981 aacgcgagaa aaaccaacag aagtacgggg cgttgctgat gcataggcta attattatcg
     2041 gtaacttttt ttaagactcg cagcggggcc ggtcagcgat tttaagcagc gcaattcgat
     2101 ggggaagtga attcgtgggg aaatcagctg taaaaacagg ccctaaggct gcggttcgcc
     2161 cggggtgcag ccgatcttac tgaagcctgg cgctaaacgc cagcagaagg tattgtgctg
     2221 ccgcgattcc accggttcgc agcattacca ccaaggaggc agcatgtatc agctaatcgg
     2281 ggcg
//
LOCUS       DAOQGS010000649         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31145_length_2276_cov_3.643854, whole genome shotgun sequence.
ACCESSION   DAOQGS010000649 DAOQGS010000000
VERSION     DAOQGS010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31145_length_2276_cov_3.643854"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1938)
                     /locus_tag="PKZ52_17290"
     CDS             complement(250..1938)
                     /locus_tag="PKZ52_17290"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HNG61581.1"
                     /translation="MNINASIVDQQLEGIVQNHSSLLPKSNDPIKLKSTAFVLLCAAT
                     LFDADITEAADWLTEGGGDAGVDAMHVSDVDDGEFTVTLFQGKYKHNNLDGEANFSEN
                     GIKSAITTIATLFDPNKQVEMNDRLKPKVEEVRSLIRDGYIPLVRMVLCNNGITWKQE
                     AQHYIDQAGFSSDQVQWRHFNHDDIVNVLKRKKSVDDAITLRGSALIEDFNFRRVLIG
                     KVSVHEVAELFNRNGDLLLERNIRRYLGLNRNRVNSSIHETLLDESKRGNFYFFNNGI
                     TMICKKFRHNALQGKDFQLKLEGIQVINGGQTCKTIQQTLNQPDLLRNFDDTYVLLRL
                     YELAEDDSEFVQDITFATNSQNPVDLRDLRSNDPVQVQLELGLKGLGYTYKRKRDEVA
                     VASDQLTSSVVAEATLAIWRRKPHQAKFKRSELFGKLYDPIFATLNPEQALLATIIFR
                     LVENERKRPQIENPPAFLPYASHYIAMLIGDELLRSKSLLLEQITHKNIDELRNNLEL
                     NNIALQQSARHKIERALTELYGDRKISLQQLSATFRRGDLEEVLAGIETQQLPI"
     gene            complement(2009..>2276)
                     /locus_tag="PKZ52_17295"
     CDS             complement(2009..>2276)
                     /locus_tag="PKZ52_17295"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG61582.1"
                     /translation="PGQLPNSLSLDGLMENTVTRNEAIVNLLSRYYAAREEAGRQALI
                     ERRGEGVPKIMHASFALSGKEPEYRMVDDAELLLIIYAAAKSDL"
BASE COUNT          584 a          520 c          482 g          690 t
ORIGIN      
        1 gtccatccct ttgcgctccg ggcggttatc gtacagttca ctaaattccc ccagctccgc
       61 ctttaagcgc tcaaaatcca gggtctccac tctctcgccc tagtcatttt gagtttcggt
      121 aaaggccgac gggaagagtt ttttaagttc ttgtttgcgc tgtttagtaa tgtctaaaga
      181 atcgcctgag atttagatag tgtgatcagt catagttatt cttgctatag gggatggttg
      241 taaagattat taaataggca gttgttgtgt ctctattcca gccaacactt cttctaaatc
      301 accgcgccta aaggtggctg aaagctgctg caaggaaatt tttctatctc cataaagctc
      361 tgtaagcgct cgctctatct tatgtctagc tgactgctgc agggcgatat tgtttagctc
      421 taaattattt cgcaattcgt cgatattttt gtgggtgatt tgctccagca gcaatgactt
      481 gctccgcagc aattcgtcac caattaacat ggcgatataa tgcgaagcat agggcaaaaa
      541 tgcaggtgga ttttctattt gcgggcgttt gcgctcattc tccaccaacc taaaaataat
      601 ggtagccagt aatgcttgtt cggggtttag agttgcgaaa atggggtcgt acaatttgcc
      661 aaataattcg ctacgtttga attttgcctg atgtggcttt cttcgccaaa tggccaatgt
      721 tgcctccgcc actaccgaac tagtcaactg atctgatgcg accgccactt cgtcacgttt
      781 gcgtttgtac gtatagccta agccttttaa acccagctct aattgcacct gaacggggtc
      841 gttggagcgt aaatcgcgca aatctactgg attttggcta ttggtggcaa aggtaatatc
      901 ttgcacaaat tcgctgtcat cttcagctag ctcataaagc ctgagcaaca cgtaggtgtc
      961 gtcaaagttt ctaagcaaat cgggctggtt caacgtttgt tgaatggttt tgcaggtttg
     1021 acctccatta atgacttgga taccttcaag ttttagctgg aaatccttgc cttgaagagc
     1081 attgtggcgg aactttttgc atatcatggt tatgccgtta ttaaagaaat aaaaattacc
     1141 tcgcttggat tcatccaaca gtgtctcatg aatagagctg ttaactcgat tgcggttgag
     1201 ccccaaataa cgacgaatat tgcgctccaa taataggtcg ccatttcgat taaataattc
     1261 cgccacttca tggacggaaa ccttgccgat caacactcgg cgaaaattaa aatcttcaat
     1321 caatgcagaa ccacgcaagg taatggcatc atcgaccgat tttttgcgct ttaatacatt
     1381 cacgatgtca tcatgattaa aatgccgcca ctgaacttga tcactggaaa atccagcttg
     1441 atcaatatag tgttgcgcct cttgtttcca tgtaatgcca ttgttacaca gcaccattcg
     1501 caccaatggg atatatccat cgcggattaa tgaacggact tcctccacct tgggttttag
     1561 gcgatcattc atctcaactt gtttattggg gtcaaataag gtggcaatgg ttgtaatggc
     1621 agatttaatt ccattctcgg agaaattagc ttccccatct aagttgttat gcttatattt
     1681 tccctgaaaa agggtaacag taaattcgcc atcatcaacg tcactgacgt gcatagcatc
     1741 aacaccggca tccccgccac cttcggttaa ccagtccgcc gcttctgtaa tatccgcatc
     1801 aaataaagta gccgcacaga gcaacacaaa agccgttgat tttaacttta tcggatcatt
     1861 agatttaggt aataggcttg aatgattctg tacaatgccc tctagctgct gatcgactat
     1921 gcttgcgtta atattcatta ttgccaccaa gaattattta aatttaatcg tattcagcac
     1981 aaaaccttga tctttggtag ggtgtgccct ataaatcact ttttgctgca gcatatataa
     2041 ttagtaaaag ctccgcatca tctaccatgc ggtactctgg ctctttaccg ctaagggcaa
     2101 agcttgcatg cataattttg ggcacacctt cacccctgcg ctcaatgagg gcttggcggc
     2161 ctgcctcttc gcgggcggca taatagcggc tgagtaaatt cactatggct tcgttgcggg
     2221 taacagtgtt ctccattaac ccgtcgaggc tgaggctatt ggggagttgc cctggg
//
LOCUS       DAOQGS010000650         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31283_length_2270_cov_5.060948, whole genome shotgun sequence.
ACCESSION   DAOQGS010000650 DAOQGS010000000
VERSION     DAOQGS010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31283_length_2270_cov_5.060948"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKZ52_17300"
     CDS             complement(<1..306)
                     /locus_tag="PKZ52_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604476.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNG61583.1"
                     /translation="MRVLGIETSCDETGVALYDSAEGLLAHRLYSQIGLHSEYGGVVP
                     ELASRDHVRKLVPMVEEVLAAANCAPSSIGAVAYTAGPGLIGALLVGACMGRALAYAW
                     "
     gene            520..735
                     /gene="rpsU"
                     /locus_tag="PKZ52_17305"
     CDS             520..735
                     /gene="rpsU"
                     /locus_tag="PKZ52_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNG61584.1"
                     /translation="MPSVKLKENEPFDIALRRFKRSCEKAGVLAEVRRREFYEKPTTV
                     RKRKAAAAVKRHAKKVSRESRKFTRMY"
     gene            796..1245
                     /locus_tag="PKZ52_17310"
     CDS             796..1245
                     /locus_tag="PKZ52_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNG61585.1"
                     /translation="MSTEIKQRVNAAVKDAMRAQAKDRLGVLRLIAAEYKKVEVDERI
                     EVDDARALQLLDKMVKQRRDSVSQFIAGGRPELAEKETFEISVIQEFLPQSLSDEELV
                     TLIKEAIAETGAASAQDMGKLMAIIKPKAQGRCDMSKVSQLIKAQFA"
     gene            1306..>2270
                     /gene="dnaG"
                     /locus_tag="PKZ52_17315"
     CDS             1306..>2270
                     /gene="dnaG"
                     /locus_tag="PKZ52_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNG61586.1"
                     /translation="MSRIPSSFIDDLLNRLDIVDVVDHRVKLKRSGKNYMACCPFHQE
                     KTPSFTVSPDKQFYYCFGCGASGNALGFIMEYDHLGFTDAVESAAKVLGLTVPYEDTQ
                     PGKAPKKESNLYTLLEQASQFYKQQLREHPERKTALTYLQTRGLTPEICALFGIGYAA
                     AGWDNLLRALGPNEQAQKQLLDAGLITENEDKTRKYDRFRQRIMFPILDVRGRTIGFG
                     GRILGSETTTAPDGSKIKAGGPKYLNSPETEVFHKGRELYGLYQARMSNRHLQRLLVV
                     EGYMDVIALAQFDISYAVATLGTACGEDHLQLAFKYCSEIVFCFDG"
BASE COUNT          588 a          658 c          562 g          462 t
ORIGIN      
        1 ccaggcatag gccaaagcgc gccccataca agcccccacc agcagcgcgc caattaaccc
       61 cggccctgcc gtataagcaa cagcgccgat ggaactgggc gcgcagttgg cggcggcgag
      121 tacctcttcc accataggca ccagtttgcg cacatggtcc ctcgatgcca actcaggcac
      181 tacgccgcca tattcactgt gcaaaccgat ttgcgaatac aaccgatggg ctaacaatcc
      241 ctctgcagaa tcgtacagtg caacccccgt ttcatcgcag gaggtttcaa tacccaacac
      301 acgcataaca acacccgatt taaaaagacg cgccatggta ggccccaact caagctcagc
      361 caagcgttta aatctgtcag gctagttttt cacggtatgc actgagagca attcaactaa
      421 aatttaactt gcgatggtgc tggctgaggt ttagaatccg gcacccttaa ataccggtgg
      481 cacttcgctg ccctattcaa aatccaaaca ggtagctcta tgccctcagt aaaactcaaa
      541 gaaaacgaac cttttgatat cgccctgcgc cgttttaagc gctcatgtga gaaagccggt
      601 gttctggctg aagttcgccg ccgcgagttc tacgaaaaac ccaccacagt acgcaagcgc
      661 aaagctgctg ctgctgttaa gcgtcacgct aaaaaggtat ctcgcgagtc tcgcaagttt
      721 acccgtatgt actaatcgcc aggcgtttag ccctggcacc caaggccggg caggcgcccc
      781 gaaagagcat actttatgtc gactgaaatc aaacaacgcg ttaatgctgc ggtgaaagat
      841 gccatgcgtg cgcaagcgaa agatcgcttg ggcgtattgc ggttaatcgc agcagaatac
      901 aaaaaggttg aagtggacga gcgcattgaa gttgatgatg cccgcgctct gcaactgcta
      961 gataaaatgg tcaagcagcg tcgcgactcc gtcagccagt tcatagccgg tggccgccct
     1021 gagctggccg agaaagaaac ctttgaaatc tcagtgattc aagagttttt gccgcaatcc
     1081 ttaagcgatg aagaactggt aacactcatt aaagaagcga tcgccgaaac gggcgccgct
     1141 tccgcccagg acatgggcaa gctgatggcc atcatcaagc caaaagccca gggccgctgc
     1201 gacatgagca aagtaagcca gctgatcaaa gctcagtttg cttaagcggc caaccggtat
     1261 cgagacaccc agcattgaga taagccgcct agcggcttat tttttatgag ccgaatcccc
     1321 tcctcattta tagacgacct actcaaccgc ctcgacatag tcgatgtggt ggatcaccgc
     1381 gtgaagttaa agcgcagcgg caagaactac atggcctgct gcccgttcca tcaggaaaaa
     1441 accccttcat ttaccgtcag ccccgacaaa cagttttatt actgctttgg ctgcggcgcc
     1501 tcgggcaatg ccctgggctt tattatggag tacgaccatt taggctttac cgacgcggtg
     1561 gaatcggcgg caaaagtgct cggcctgaca gtaccctacg aggataccca gccaggcaaa
     1621 gcccccaaaa aagaatccaa cctttacacc ctgctggagc aagccagcca gttttacaaa
     1681 caacaattgc gcgagcaccc tgagcgcaaa accgccctca cctatttgca aacccgcggc
     1741 ctgacgccgg aaatctgcgc cctgtttggc ataggctacg ccgctgcggg ctgggataat
     1801 ttactcagag ccctaggccc gaatgagcag gcacaaaaac aactgctcga tgccgggctc
     1861 attaccgaaa acgaagataa aacccgcaag tacgaccgct ttcgccagcg cattatgttc
     1921 ccgattttgg atgtgcgcgg ccgcaccatc ggctttggcg gccgcattct gggcagcgaa
     1981 accaccaccg cgccggatgg cagcaaaatc aaagccggtg ggcctaagta tttaaactca
     2041 ccggagaccg aagtctttca caagggccgc gagctgtacg gcctctatca agcgcgcatg
     2101 tccaaccggc atttgcagcg cctattggtg gtggaaggtt atatggatgt aattgccctg
     2161 gcgcagtttg acatcagtta cgccgtggcc accctcggca ccgcctgcgg agaagatcac
     2221 ttgcagctgg cctttaaata ctgctcggaa attgtcttct gttttgacgg
//
LOCUS       DAOQGS010000651         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31406_length_2265_cov_4.537104, whole genome shotgun sequence.
ACCESSION   DAOQGS010000651 DAOQGS010000000
VERSION     DAOQGS010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31406_length_2265_cov_4.537104"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..976)
                     /gene="serC"
                     /locus_tag="PKZ52_17320"
     CDS             complement(44..976)
                     /gene="serC"
                     /locus_tag="PKZ52_17320"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNG61587.1"
                     /translation="MTPYRFNFSAGPGALPESVLLLLQEAIHCVPELGLSVLGVSHRS
                     DWFEAVVAQTEANVRALLNLPDNYHVLLLQGGSSLQFSMIPMLLLRGRKQPAEYIDSG
                     YWSAKAIPEARREGPVTVLWSGKEAGYKRLPQSQELNCSAQASYLHYISNETVEGLQF
                     NFLPGHDGVPRICDMSSDFLSRPFDAKKFSLIYAHSQKNLGPAGVTLVVLDDGLLAQA
                     PDDLHTILDYRPHAKAHSIYNTPPVFAIYALMLITRWLLHEIGGISNMGILNAQKASL
                     LYQLLDASDGFYQPRAHRADRSLMNVVFNLPSPL"
     gene            1743..>2265
                     /locus_tag="PKZ52_17325"
     CDS             1743..>2265
                     /locus_tag="PKZ52_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61588.1"
                     /translation="MPTVLRQDFRTRIIKAGAYTNPNAATMVVGGNVVNAPDNNSPLL
                     QGANGAAMVPGGVGGVGAHYRQPLGIKFSLEMGVDPISGGRVALFRPRGNHEQAAYLP
                     YCDNYICSLILPPNGVDYFFTDNLSGCAIYIDQVHGSRDLVLYHANARATQSGPGGDP
                     AATLLMDNMHNAAM"
BASE COUNT          584 a          527 c          567 g          587 t
ORIGIN      
        1 cgtaaaggcc cgcagcatct gctgcctgaa taaattgctg ctcttataat ggcgagggca
       61 aattaaaaac cacattcatc aaagagcgat ccgccctgtg ggcgcggggc tgataaaatc
      121 cgtcgctggc atccagtagc tggtacagca agctggcttt ttgcgcattt aaaatgccca
      181 tattggatat gccgcctatt tcatgcagca gccagcgggt aattaacatc agcgcataaa
      241 tggcaaaaac cggtggcgta ttgtaaatag aatgggcctt ggcatggggg cggtaatcaa
      301 gaatagtatg caaatcatcg ggcgcctggg ccagcaggcc atcatctaat acgactaggg
      361 ttacacctgc ggggccgaga tttttttgcg agtgggcata aattaaggag aatttttttg
      421 cgtcgaaagg gcggcttaaa aaatccgatg acatatcaca aatgcgtggc accccatcat
      481 ggccgggtaa aaaattaaat tgcaagcctt caacggtttc attggaaata taatgcaagt
      541 aactggcttg cgcggaacaa tttaattcct ggctctgcgg caggcgttta taacccgcct
      601 ccttgccgct ccacaaaacc gtaactggcc cttcgcgccg ggcctccggt atagctttgg
      661 cgctccaata gccgctatct atatattccg caggctgctt gcggccgcgc aataacagca
      721 taggaatcat cgaaaattgc aagctactac cgccttgcaa taaaagcaca tggtagttat
      781 ccggtaaatt taataatgca cgcacattgg cttccgtctg tgcgacaaca gcctcaaacc
      841 aatccgacct gtggcttacg cccagtaccg acaagcccaa ttcaggaaca cagtggattg
      901 cttcttgcaa aaggagtaaa acggattctg gcaaagcgcc ggggcctgcc gaaaaattaa
      961 agcgataagg tgtcattatt gattgccgca agggatattg ggtttaactg catccctcgc
     1021 ttcaatccgt gaatcacccg gcagtaaacg cgcaagggtg aataaaaata ctagcattta
     1081 aatgccaaaa gcggcaatta gggaacctct gaaaaatgta ggcggcgaag cgctggctgg
     1141 gtgaatttgg caaaaaaagc ggacgactgc agggatgcgg gaggtagagc aatgcaggag
     1201 caattgccga gtttacacgc agtaaatgag catttttttg gcaaattcaa cgccgccagg
     1261 gcgagtaagg taatttttca gggattcctt agaatccgtt ataagtaatt cgctgatggt
     1321 gtcgatgctg catcgctcga aaggcgtctg ctgaatggcg gctctgcttt tagacgcttg
     1381 taaaaattgt ctcaatcgct ctcgttgcta tgagttcagt gaataagcaa tttgatttct
     1441 gtaaaaccgg ctttttcctt atgtgagcca attttttcaa accagcacct ggccgccaat
     1501 acattttggt gggattaaat tttttttaca attatagcat cctctatgcc gattgtttgt
     1561 tcggccctta tttgatagat cgtgcctgca cgcgggtgtc ttttaaaatt atgcagttct
     1621 tcgcgccaaa atctgcagtg ctcaatgccg ccatttaatt ctgcagtgtg gaatagagtg
     1681 attctggcgc atctgggtgt tagctattat gataaacgcc ctaatcaatt ggagcccaac
     1741 ccatgcctac cgtgttacgt caagatttcc gcacacgaat aataaaagca ggtgcttaca
     1801 caaatccaaa cgcggccact atggtggtgg gcggcaatgt ggttaatgcg ccggataaca
     1861 atagcccgct tttgcaaggt gccaacggcg cggccatggt gcccggtggt gttggtgggg
     1921 tgggggccca ttatcgccag cctttaggca ttaagttttc tttagaaatg ggggtagacc
     1981 ctatctccgg tgggcgcgta gctttatttc gcccgcgggg taatcatgag caggcggcct
     2041 atttacccta ttgtgacaat tatatttgct cgttaatttt gccgcccaac ggcgtggatt
     2101 atttttttac ggataatctc tctggttgcg caatttatat tgatcaggta cacggctccc
     2161 gtgatttggt gttgtaccac gccaatgccc gcgccactca aagtggcccc ggcggcgatc
     2221 cagcggctac cttattaatg gataatatgc acaatgccgc catgg
//
LOCUS       DAOQGS010000652         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31572_length_2257_cov_3.985922, whole genome shotgun sequence.
ACCESSION   DAOQGS010000652 DAOQGS010000000
VERSION     DAOQGS010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31572_length_2257_cov_3.985922"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..>2257
                     /locus_tag="PKZ52_17330"
     CDS             591..>2257
                     /locus_tag="PKZ52_17330"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HNG61589.1"
                     /translation="MQNVFTFPDGRNFALCYAQTPLGASHRQDTPLRSPQDIDQLMAY
                     LGNAKLAGIWRDYYLDQARNKQASPTAPISEMRKRIKEDLWKGRLYFIPRDNPEDKPD
                     NIDQAIRTARTNLQMHLRTIIQDEQKEKAKLEADYEKLSATEKAALSARKAATGLGKA
                     AKDFLIWAGDLADVADINRRAIRIIDAAKKTYWDQNKKTWGENLAQAEYKELVELLGF
                     DPAKITRAQVEEAYELANLIWNDIETRNILKQFALDYAKAQHHTEWTEMAGGLAFEVI
                     LTIILAAITGGAGGVAVVAKNALLLGRLEKAGQSLLTIGKKLKSLKIRESRKVIKTDS
                     APTASGPAPKSKYDKADETQHNAGAKPATQPQPQAAPAQKPAEGKGEIKHVEPAKVTP
                     ESPAGSPVASACTTEGCPISMVSGEELLPLVDGRLPGPLPFEWKRTYRSAHSRDVGLG
                     HGWTHAGCEYIEEQAAQLIYWDDEGRMISMPRPDIGQTTHLINEQLRINQIADGIYRL
                     IKKQEPIKEFTRTGDANRARLQRILHPTAAFVLQFEYENNQLSAINS"
BASE COUNT          637 a          613 c          528 g          479 t
ORIGIN      
        1 cgccaataac gtcaatctat tagtagaaac ggtgatcctc aactatatgg ccctgcacgg
       61 caaacagggt ctcttcgccg acccgaatta tttcccccgc cacggcttgg gaagcacggc
      121 taactggacg aggctcacgg cgtttaaccc gattgtttag tgagtcatag agtctgcgca
      181 ttaaacggtt ttgcgcccgc ccccttactt aagacatgtt attaagttaa ggcggcacgc
      241 ccccaaattc cccgctggag aggagggcgg ggtggttgcg gtgtatctcc atggggagtg
      301 gaattgtttc ttacccttga gcgaaagttg tacgcaaaaa ataccgcggc gccctttgca
      361 aaggagggaa atgttagtcc gcaccaatac gataaatgtc cgccaatact caaattgatg
      421 aaattgcttt gagggggatt gggcttaagt ccctcatcca aggctacttt tgtcccattc
      481 ccgcaaccta ccctgcggcg caatttacta atgcccacct acaggttacc cttatcgcta
      541 gatgagccaa aacgcttata cgatttgaaa tgttgttcca aggaatcctc atgcagaatg
      601 tttttacctt ccccgatggc cgcaactttg cgctctgcta cgcccaaact cccctcggcg
      661 ctagccaccg gcaagacacc ccgctgcgtt ctccccaaga tatagaccaa ttaatggcct
      721 atttaggcaa tgcaaagtta gcgggtattt ggcgggatta ttatctcgac caggcccgca
      781 acaaacaggc atccccaacc gcgcccatca gcgaaatgcg caagcgcata aaagaagatt
      841 tatggaaagg ccgtttgtat tttatccccc gcgacaaccc ggaggataaa ccggataata
      901 tcgaccaagc catccgcacg gcgcgcacca atttgcaaat gcacctgcgt accattattc
      961 aagatgaaca aaaagaaaaa gctaaattag aagctgacta cgaaaaactc agcgctacgg
     1021 aaaaagccgc actttccgcg cgcaaagcgg caactggcct tggcaaagcc gccaaagatt
     1081 ttttaatttg ggccggtgat cttgcggatg tggccgatat caatcgccgt gctatccgca
     1141 taattgatgc cgcaaaaaaa acctattggg accaaaataa aaagacctgg ggcgaaaatc
     1201 ttgcgcaggc cgaatataaa gaattagtag aacttctcgg ttttgacccc gccaaaatca
     1261 cccgcgccca ggttgaagag gcctatgaac tcgccaattt aatttggaac gatatcgaaa
     1321 cccgcaacat cctcaaacaa ttcgccctcg attacgccaa agcacaacac cacactgaat
     1381 ggacggaaat ggccgggggc ttggcatttg aagttattct cacgattatt ctcgctgcaa
     1441 taacaggcgg cgctggtggc gttgctgtgg ttgcaaaaaa tgccctgctg cttggacgcc
     1501 ttgaaaaggc cggccagagc ctgcttacca ttggcaaaaa acttaaaagc ctgaaaattc
     1561 gtgaaagccg caaagtaatt aagaccgata gcgccccgac agcatccggc cctgcaccta
     1621 aatcgaaata cgacaaggca gacgaaactc agcacaatgc gggggcaaaa cccgctacac
     1681 aacctcaacc ccaggcggct cccgcacaaa aacctgccga aggtaagggc gaaataaaac
     1741 acgtcgaacc cgctaaggtc acaccagaat cgcctgcggg gtcgcctgtt gcctccgcct
     1801 gtactaccga aggttgcccc atcagcatgg tctccgggga agagttgttg cccctagtgg
     1861 atggacggtt gccaggcccc ttgccctttg aatggaaacg cacctaccgc agcgcccaca
     1921 gtcgcgatgt cggcttgggc cacggttgga cgcacgctgg ctgtgaatac attgaagagc
     1981 aggcagcgca gcttatttat tgggacgatg aaggccgcat gattagcatg ccgcgtccgg
     2041 acattggcca aactacccat ttaattaacg aacaattacg tatcaaccaa attgccgatg
     2101 gcatctatcg gctaattaaa aaacaagagc cgataaaaga atttacacgt acgggcgatg
     2161 ccaatcgagc gcgtttacag cgaatactcc atcccacagc tgcgtttgta ctgcaattcg
     2221 aatatgaaaa taatcaatta agcgcaatta acagtaa
//
LOCUS       DAOQGS010000653         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31652_length_2254_cov_3.116417, whole genome shotgun sequence.
ACCESSION   DAOQGS010000653 DAOQGS010000000
VERSION     DAOQGS010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31652_length_2254_cov_3.116417"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKZ52_17335"
     CDS             complement(<1..262)
                     /locus_tag="PKZ52_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG61590.1"
                     /translation="MNPNAAQSSAQPSENFVFILLISCVATIGGFLFGFDSGVINGTV
                     EGLKVAFNADSIGTGFNVASILLGSAIGAVKAGVLADRYGRRS"
     gene            578..2065
                     /locus_tag="PKZ52_17340"
     CDS             578..2065
                     /locus_tag="PKZ52_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNG61591.1"
                     /translation="MHFLGIDVGSSSVKLSILDGESGKAVASTTYPESELAINSPEPG
                     WAEQDPETWWHCIAQGCAVLFNKPGVDPKRIGAIGISYQMHGLVLVDKQQNILRPSII
                     WCDSRAVEIGNKALVDIGADYCFGHLLNSPGNFTAAKLRWVQENEPSVFARIHKIMLP
                     GDFIAMKLSGEITTTASGLSEGTLWDFEQHTVAKQLLAHWNIDAQLLPDICPSIGVQV
                     KVNAQAAQLLGIPEGIPIAYRGGDQPNNAFSLNVLNPGEVAATAGTSGVIYGVTDQKA
                     ADRQSRVNTFLHVTNTQQAPRNGVLVCVNGAGRSYSWLRQLLGANGHAIDYGQLNAWA
                     ASTPVGADGLIFHPFGNGAERIFQNRTIGASFSKLELNRHGVGHMIRATQEGIVFALN
                     QGFDVLKSLGGQCNVVRVGYGNMFLSDVFAQAFANTTQAAVECYNTDGAEGAARAAAI
                     GSGFYASTQEAFGGLVKLKVIEPQADLMAAYAEAYGRWVDALPAV"
     gene            2069..>2254
                     /locus_tag="PKZ52_17345"
     CDS             2069..>2254
                     /locus_tag="PKZ52_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61592.1"
                     /translation="MSTEKDYPFFELVTGDQKARLELLSSHGGIINALVFTDSQGLQH
                     NVIAGFNSLQAIEQDQYY"
BASE COUNT          522 a          601 c          607 g          524 t
ORIGIN      
        1 tgctgcgccg gccgtagcga tccgccagca cacccgcctt aacggcgccg atggctgagc
       61 cgagtaatat agaagcaacg ttaaagcctg tgccgatgga gtcagcatta aaagcgactt
      121 ttaagccttc cacggttcca ttgatcaccc cgctgtcaaa cccaaacaaa aagccgccga
      181 tagtggcgac acagctaatc agcagaataa aaacaaaatt ttcagaaggt tgcgcagagg
      241 attgcgcggc gtttgggttc atagttggtc tcaatcgtca gtcttattgt taatgaaata
      301 atggcgattg tgggaggctg gcattgatgg tcaccagatt ccacaaagcc gcgtggcggc
      361 agctgcacta tagcactttc ctctgctaat actgcatgca cagcatgggc tgtgagtgtg
      421 acttggcgcg gaaaaaccgc ctcgtccggc gcatttttta tcgaatttgg gccctgcggt
      481 gctacaatcg cgcgtttttt aatccctgcc gggcgctcaa aacgagccct ttgcgctcag
      541 tgcatcccgt taaatcgaat taatataggc caaatccatg catttcctcg gtatcgacgt
      601 aggtagttcc tcggttaaat tatctatttt ggatggcgaa agcggcaaag ccgtcgcctc
      661 aactacctat cccgaatcag aactcgctat caacagcccc gagcccggct gggccgagca
      721 ggatcctgaa acctggtggc actgtattgc gcaggggtgt gcagttctct tcaacaagcc
      781 cggcgttgat cccaagcgca ttggcgccat cggtatctcc tatcaaatgc atggcctggt
      841 gctggtcgat aaacagcaaa atattctgcg cccctccatc atttggtgtg atagccgcgc
      901 cgttgagatc ggcaataagg ctctggtgga tataggggcc gattattgtt tcggccatct
      961 actgaattct cccggcaatt tcaccgcggc taaattgcgc tgggtgcagg aaaacgagcc
     1021 cagtgtattt gcccgcattc ataaaataat gctgccaggg gatttcatcg ccatgaaact
     1081 ctccggcgaa atcaccacca ccgcatcggg tttgtctgaa ggcaccttgt gggattttga
     1141 acaacacacg gtggcgaagc agctgctggc ccactggaat atagatgctc agttacttcc
     1201 ggacatctgc cccagcattg gcgtacaagt aaaggtaaat gcacaagcgg cgcagttgtt
     1261 gggtattccg gagggtattc caattgccta ccgcggcggt gatcagccga ataatgcctt
     1321 tagtttaaat gtgctgaacc cgggtgaagt tgccgccacg gcgggaacct ccggagtaat
     1381 ttacggcgtc accgatcaaa aagccgctga tcgccagtcc cgcgtgaata cctttttgca
     1441 tgtgaccaat acacaacaag cgcctcgcaa tggagtattg gtgtgtgtca atggcgccgg
     1501 tcgctcctac agctggctgc gccagctctt aggtgccaac ggccacgcta tcgattacgg
     1561 ccagttaaac gcctgggctg ctagcactcc cgtgggggcc gacgggctta ttttccatcc
     1621 ctttggcaat ggcgctgagc gcattttcca aaatcgcacc atcggcgcga gcttctccaa
     1681 attggaatta aaccgccacg gcgtgggtca catgattcgc gccacccagg agggcatagt
     1741 gttcgccctc aatcagggtt ttgacgttct caaaagcctc ggtgggcaat gtaatgtggt
     1801 gcgggtaggc tatggcaata tgtttttaag cgatgtattt gcccaggcat ttgccaatac
     1861 cacccaggcc gccgtggagt gttacaacac cgacggcgcc gagggtgctg cccgtgcggc
     1921 agcgatcggc agcggttttt acgctagcac ccaggaagcc tttggcggcc tggtgaaatt
     1981 aaaagtgatc gaaccccagg cggatttgat ggccgcttat gccgaagcct atggccggtg
     2041 ggttgacgct ttgccagcgg tataaatcat gagtacagaa aaagactacc cgttttttga
     2101 gctggtcaca ggcgaccaaa aagcgcgcct ggagctgctg tcgagccacg gcggaattat
     2161 taacgccctg gtattcacgg acagccaggg tctgcagcac aacgtcattg caggctttaa
     2221 ctcgctgcag gccatcgagc aggatcagta ttat
//
LOCUS       DAOQGS010000654         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31834_length_2246_cov_3.938384, whole genome shotgun sequence.
ACCESSION   DAOQGS010000654 DAOQGS010000000
VERSION     DAOQGS010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31834_length_2246_cov_3.938384"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1148)
                     /locus_tag="PKZ52_17350"
     CDS             complement(51..1148)
                     /locus_tag="PKZ52_17350"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HNG61593.1"
                     /translation="MRILLLSAYDAPSHRYWRQGLIDQLSDYRFTCLTLPPRHFNWRI
                     RGNPISWLDAPQLTAAYDLILATSMVDLATLRGLVPALSRTPCIYYCHENQFAYPESP
                     RQMRPLEPLMVTLYGALAADKVVFNSHWNQQSFFAGVAELLNKMPDKVPAGLVSRIEA
                     KACVLPVPLRQSAPAVKTAAPFSPERPLHILWNHRWEFDKGPDLLLALIEAIPEGLPL
                     RWHIVGQGFRSQPAEFTRMQTLLDGQLASWGFAEAAAYQEILAQSHLVLSTAWHDFQG
                     LAILEAASLGAVPLVPAGLAYPQWFSACHCYGTAEDFGRRDIAALAASAAAAITAYAE
                     GTKALVPADTRQWQWPELKPAYRQLFESMGR"
     gene            complement(1152..1352)
                     /gene="iscX"
                     /locus_tag="PKZ52_17355"
     CDS             complement(1152..1352)
                     /gene="iscX"
                     /locus_tag="PKZ52_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNG61594.1"
                     /translation="MPMKWTDVNAIAIELAETHSDVDPQWVNFVDLRAWVLALPDFDD
                     DPNRCGEKVLEAIQMAWLDEVN"
     gene            complement(1399..1845)
                     /locus_tag="PKZ52_17360"
     CDS             complement(1399..1845)
                     /locus_tag="PKZ52_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61595.1"
                     /translation="MRDSAIASQRLPWHGWTLAVIYLLYSLAAAFDYVMSFVQGADYY
                     RASGMTDAQVVYFSSLPMWVLAAWTLSVWGGLLAPLALMARRRFSRELFVLSLLGVLL
                     YILYTLVLSAGREAMGGLWFMPIAIAVITAGFIVYCHQLIKRRVLV"
     gene            complement(1863..2210)
                     /gene="fdx"
                     /locus_tag="PKZ52_17365"
     CDS             complement(1863..2210)
                     /gene="fdx"
                     /locus_tag="PKZ52_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNG61596.1"
                     /translation="MPPPGKITFLPHHEICPAGATIEANPGESICEAALRNRIGIEHA
                     CEMSCACTTCHVIIRQGYFSLEPPDELEEDYLDKAWGLEADSRLSCQALVGDEDLVVE
                     IPKYTINMVSEKH"
BASE COUNT          526 a          632 c          643 g          445 t
ORIGIN      
        1 agattcaagc attaggcagc cgtagtagag ttcaactgcg gtttttaggt ttaacgtccc
       61 attgattcaa atagttgccg ataagcgggc tttaactctg gccactgcca ttgccgggta
      121 tcggccggga ctagtgcctt tgtgccttcg gcatacgcgg tgatggcagc ggcagcactg
      181 gcggccagcg ctgcaatatc acggcggcca aaatcttctg ccgtgccgta gcagtgacaa
      241 gcactgaacc actgcgggta cgcaagcccc gcaggcacca aaggcacagc gccgaggctt
      301 gccgcttcca atatcgccag cccctgaaaa tcgtgccaag cggtggagag taccagatga
      361 ctttgcgcca aaatttcctg ataagccgct gcctcggcaa aaccccagct tgccagttgc
      421 ccatcaagca gagtttgcat acgggtaaat tctgccggtt gactgcgaaa cccctgcccc
      481 actatatgcc agcgcagggg caagccctcc gggatagcct cgattaaagc caatagcagg
      541 tccggcccct tatcaaactc ccagcggtgg ttccaaagga tgtgcaacgg ccgctctggg
      601 ctaaagggcg ccgccgtttt aacggcgggt gcactttgcc gcaggggaac gggcaacaca
      661 caggccttgg cttcaatacg gcttacaagc ccagcgggga ctttatcggg cattttgttt
      721 aacagctccg ctacgccagc aaaaaaactc tgctgattcc agtgcgagtt aaacaccact
      781 ttatccgccg ctagtgcgcc gtaaagcgtc accattaggg gctctaaagg cctcatttga
      841 cgcggggatt caggataggc gaactgattt tcgtggcagt aatagatgca aggcgtgcgg
      901 ctaagggcgg gcactaggcc gcgcagggtg gccaaatcca ccatggaggt ggcgaggata
      961 agatcgtagg cggcagttag ctgcggggca tccagccagc tgatggggtt gccgcgaatg
     1021 cgccagttga aatggcgcgg cggcagagtc aggcaggtaa agcggtaatc gctgagctgg
     1081 tcgatgaggc cctggcgcca atagcggtgg ctgggggcgt cgtaggcgga taacagcagt
     1141 atccgcaccg tttaattcac ttcgtctaac caggccattt ggatggcttc caacaccttt
     1201 tcaccgcagc ggttggggtc gtcgtcaaaa tctggcaggg ccagcaccca ggcgcgcagg
     1261 tcaacaaaat tcacccactg agggtccaca tcgctgtggg tttcggcaag ctcgatggcg
     1321 atggcgttta cgtcggtcca tttcataggc atgggggcac ctctttcgca attaagtgtt
     1381 tgggggcttg agtcggcgtc aaaccagaac ccggcgcttt atcaactgat ggcaataaac
     1441 aataaaacct gcggtgatga cggcgattgc gatgggcata aaccacagcc cacccatggc
     1501 ctcgcgccca gcggataaca ccagggtgta aaggatgtag agcaacacac ccaacagaga
     1561 gagcacaaat aactcccggc taaaacggcg gcgggccatc agtgccagtg gggccaacaa
     1621 gccaccccac accgagaggg tccaggcggc taacacccac atgggcagcg atgaaaaata
     1681 caccacctgt gcatccgtca tgcccgacgc ccggtaataa tccgccccct gcacaaagct
     1741 cattacataa tcaaaagccg ccgcgaggct gtagagcaag taaataacgg ccaatgtcca
     1801 gccgtgccag ggtaggcgct gggaggcgat tgcactgtca cgcataaatc gtccctatgg
     1861 ggttagtgtt tctcgctcac catattgatg gtgtatttgg ggatctccac caccaggtct
     1921 tcatccccca ccagggcctg gcagcttaaa cgggaatccg cctccagacc ccaggcttta
     1981 tccaggtagt cctcttccaa ttcatcaggc ggctctaggg aaaaatatcc ttggcgaata
     2041 atcacgtgac aggtggtaca ggcgcagctc atttcgcagg cgtgttcaat gccaatgcga
     2101 ttgcgcaggg ccgcctcaca gatactctcc ccaggattgg cttcaatggt tgcccccgcc
     2161 gggcaaattt cgtggtgggg caggaacgta attttgcccg gcgggggcaa ccattgaagc
     2221 caatcctggg gagagtatct gtgagg
//
LOCUS       DAOQGS010000655         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_31903_length_2243_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAOQGS010000655 DAOQGS010000000
VERSION     DAOQGS010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31903_length_2243_cov_4.000000"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PKZ52_17370"
     CDS             <1..1671
                     /locus_tag="PKZ52_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNG61597.1"
                     /translation="GSTRTREQAAMHPLSYIGSQKLEDLTRPGKHLDEMALTAWIAEQ
                     SGLTVFHIDPMKMQVAELAEVMSYQFAKRHDLLCVKITPEELTVAVGQPFVTGWEEQL
                     MQISQRRIRRVVALPSDIERYRVEFYSLARSISGASGGKGPSGVTNFEQLLELGNLKD
                     PEANDQHIVNIVDWLLQYAFDQRASDIHIEPRREKTRMRYRIDGLLHPIYEFPASVGT
                     AIVSRIKILGRMNVAEKRKPQDGRIKTKTPDGTEAELRLSTLPTAFGEKLVMRIFDPE
                     VLLRNFDDLGLNGDDLTRWVKMMESPNGIVLVTGPTGSGKTTTLYSSLKTLASDEVNV
                     STIEDPIEMVEDSFNQTQVQHNIGLDFAQGIRTLMRQDPDIIMVGEIRDLETAQMAVQ
                     AALTGHLVISTLHTNDAPTSISRLLDLGIPGYLLKATVLGVMAQRLVRTLCPHCKQES
                     DISAKDWDDLVKPWKVAKPNKVFRAVGCLECRNTGYMGRQGIYELLSMSENIANLISD
                     NTDLNALRQQAMREGMRTLRLSGAKKLAAGLTTLEEVLRVAPPSTKYQ"
     gene            1643..>2243
                     /locus_tag="PKZ52_17375"
     CDS             1643..>2243
                     /locus_tag="PKZ52_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61598.1"
                     /translation="MRLHRPNTNKLFLGLILCLCTDLACAESPHASGLPAPEAKIAAD
                     NSAATAPATDPQAATSAAAKTDPSPQEQDPALKPASNTTQTLAAATSAPTAEAPATIG
                     PNPTPAAQATMPQETASSTNLAAPLNAESARTFSGQEAGSGAIDLHPPGRFTTAEPLN
                     PSTENLLKPDPAAPPPAPLAPAAPLSLLGATLNPGSASRL"
BASE COUNT          514 a          752 c          595 g          382 t
ORIGIN      
        1 ggcagcaccc gcacccgcga gcaggcggcc atgcatccgc tgagctacat cggcagccaa
       61 aaactggagg atttaactcg gccgggcaaa catttggatg aaatggccct gaccgcctgg
      121 atcgccgagc agtcgggcct caccgtgttc cacatcgacc cgatgaaaat gcaggtggcg
      181 gaactggccg aggtgatgag ttaccagttc gccaaacggc acgacttatt gtgcgtgaaa
      241 atcaccccag aggagctcac tgtggcggtg ggccagccgt ttgtgaccgg ctgggaagag
      301 caattaatgc agatcagcca gcgccgcatc cgccgggtgg tggccctgcc cagcgacatc
      361 gagcgctacc gggtggagtt ttactccctg gcccgctcta tctccggcgc ctccggcggc
      421 aaaggcccct ccggcgtcac caattttgaa cagctgttgg agcttggcaa ccttaaagac
      481 ccggaggcca acgaccagca tattgtgaat atagtggact ggctgctgca gtacgccttt
      541 gaccagcgcg ccagcgacat tcacatagaa cccaggcggg aaaaaacccg catgcgctac
      601 cgcatcgacg gcctgttgca ccccatctac gaattccccg ccagcgtggg cacggccatt
      661 gtcagccgga ttaaaattct cggccgcatg aatgtggcgg aaaaacgcaa gccccaggat
      721 ggccgcatca aaaccaaaac ccccgacggc accgaggcgg aattgcgcct ctccaccctg
      781 cccacggcgt ttggcgaaaa actggtgatg cggatttttg acccggaagt actgctgcgc
      841 aacttcgatg atttaggctt aaacggtgac gatctcaccc gctgggtgaa gatgatggag
      901 agccccaatg gcatagtgct ggtcaccggc cccaccggct ccggtaaaac caccacgctc
      961 tattcctcct taaaaaccct cgccagtgac gaggtgaatg tcagcaccat cgaggatccc
     1021 atcgagatgg tggaagattc gtttaaccaa actcaggtgc agcacaatat cggcctggat
     1081 ttcgcccagg gcatccgcac cctgatgcgc caggacccag acatcatcat ggtgggggag
     1141 attcgcgacc tggaaacggc tcagatggcg gtgcaggcgg cgctcaccgg ccacttggtg
     1201 atctccaccc tgcacaccaa cgacgccccc acctccatct cccgcctgtt ggatttgggt
     1261 atccccggtt atttactcaa ggccactgta ttgggtgtga tggcccagcg cctggtgcgc
     1321 accctctgcc cccattgcaa acaggagagc gacatcagcg ctaaagactg ggatgacctg
     1381 gtgaaaccct ggaaagtggc caaacccaac aaggtgtttc gcgccgtggg ctgcttggaa
     1441 tgccgcaaca cgggttatat ggggcgccag ggcatttatg aattactctc catgagtgag
     1501 aatatcgcca atttaatcag tgacaacacc gatcttaacg ccctgcggca acaagccatg
     1561 cgcgaaggca tgcgcacctt gcggctgtca ggcgctaaaa aactggccgc aggccttact
     1621 accttagaag aagtactccg cgttgcgcct ccatcgacca aataccaata aactctttct
     1681 cggcctaatt ctatgcctct gcacggacct cgcctgtgca gagtcgccac acgcctcagg
     1741 cctgcccgcc ccggaggcca agatcgccgc cgacaacagt gcagcgactg cacctgccac
     1801 agacccgcag gcggctacat cagctgccgc gaaaacggat ccatcaccgc aagagcagga
     1861 tccagcttta aaacctgcgt cgaacaccac ccaaaccctg gccgccgcaa cctctgcacc
     1921 tactgccgaa gcgccagcga ccatcggccc caacccaacg cctgcagcgc aggcaaccat
     1981 gccgcaagag actgcaagca gcactaatct cgccgctcct ttaaatgccg aaagcgcccg
     2041 cacctttagc ggccaagaag cgggttccgg ggccatcgac ctacacccac cgggccgctt
     2101 taccaccgcc gaacccctaa accccagcac cgaaaacctc ctcaagccag accccgccgc
     2161 gccgccgcca gcccccctgg caccggcggc gcccctgagt ttgctcggtg ccacgctaaa
     2221 ccccggcagc gccagccgcc tgg
//
LOCUS       DAOQGS010000656         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32172_length_2231_cov_3.979320, whole genome shotgun sequence.
ACCESSION   DAOQGS010000656 DAOQGS010000000
VERSION     DAOQGS010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32172_length_2231_cov_3.979320"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..709)
                     /locus_tag="PKZ52_17380"
     CDS             complement(95..709)
                     /locus_tag="PKZ52_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61599.1"
                     /translation="MFANQAAFNDPKSRMQALGQNTRPEGAVVDSRMKPLFYLQTGGM
                     INPQRDEIKPGAILYRFASAKDGVQRGMTGGWWVHAKEFAIIENFATLNRVGVSMAAR
                     ILCCVPPEWSDMTLLVRACAREPLLAYKGLGNHVNTTAPDNLERVKMTAHNDIAARRL
                     HQLYIPGLYDCAKKTPEQVMPGALALEQHWEFTKEEAKKGWIYI"
     gene            complement(899..>2231)
                     /locus_tag="PKZ52_17385"
     CDS             complement(899..>2231)
                     /locus_tag="PKZ52_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNG61600.1"
                     /translation="MGIKSIISIWPRFEKASRYFNELDAKGYFLKDKEGKTQDGLPYR
                     NDRTGGLIDSTNPAARKWFWQHARDGVLSAGFDYPWLDETEPDLVPEGLFFSIGSGDR
                     YRNVYPLVHVEGFAEGLREYRPNVRSLILARAAYLGSQRTGGLFWSSDINPSWDTLAR
                     QVPTGLNMTASGIAYWGNDIGGWQWLPQTTSATKPALLEPSDARAVVGNNHDYPELFT
                     RWFQYGVFTPTLRIHGMRAQTEIWSFGKQAEAILAQYNRLRYQLIPYIYAQAKITYDT
                     GTPFMRALWFDFPQDAKVINLGSQYMFGDAFLVAPVTEQGQTQKAVYLPAGADWYNWW
                     TREKFSGGQTITVAAPIEQIPLFVKAGSIIPLGKLVPSTATAQEIEKILVFPGTDASF
                     TLYDDDGVSWGYQKGNFTTNELNWSEEKQELTAKGGLKGKVKHLVEVIRNK"
BASE COUNT          558 a          606 c          517 g          550 t
ORIGIN      
        1 tggcaaattc gcctagccag cgcttcgccg cctacatttt tcagaggttc cttagaataa
       61 aattggcaac tatttaaaga atcggcatca attactaaat ataaatccaa ccttttttag
      121 cttcttcctt ggtaaactcc caatgctgtt ccagcgccag tgcgccaggc attacctgct
      181 cgggtgtttt tttcgcgcaa tcataaaggc cgggaatata caattggtgt aggcggcggg
      241 cggctatatc gttgtgggcg gtcattttca cccgctctaa attatccggc gcggtggtat
      301 tcacatggtt gccaaggccc ttgtacgcca gtagaggctc ccgcgcacag gcgcgcacaa
      361 gcagtgtcat atcgctccac tccggcggca cacaacacaa aatccgcgcc gccatggaaa
      421 ctcctacccg gttaagggtt gcaaaattct caatgatggc aaattcttta gcgtgcaccc
      481 accagccgcc ggtcatcccc cgctgcactc cgtctttggc actggcaaag cggtacaaaa
      541 ttgcccccgg tttaatttcg tcccgctggg ggttaatcat gccgccagtt tgcaggtaaa
      601 acaggggctt catgcggcta tctaccaccg ccccttccgg gcgagtattt tgcccgaggg
      661 cctgcatgcg gcttttgggg tcattaaagg cagcttgatt tgcgaacata agtagattcc
      721 atggcaactg ataagattaa aatttcgcgt ttaacacagc agccggtgcg cttaatacaa
      781 taagcctaac cgcaccaaag cctgcgcagc ttaaaataac atgggcttag cggcaagtta
      841 tagctaaccc gtgccgagtt aacaaaaaat tacacattca caagcgctat tttcgtggct
      901 atttattcct aatcacctca accaagtgtt taaccttgcc ttttaaaccg cccttagcgg
      961 ttaattcctg tttttcctca ctccaattta attcattggt ggtaaaattg cctttttgat
     1021 agccccatga aacgccatca tcatcgtaaa gcgtaaagct cgcatcagtg ccgggaaaca
     1081 ccaatatttt ttcaatctcc tgcgcagttg cagtgctggg caccagcttg cctaatggaa
     1141 taattgaacc ggctttaaca aacagaggga tttgttcaat cggtgctgca acggtaatag
     1201 tttgcccgcc gctgaatttt tcccgcgtcc accagttgta ccaatctgct ccggccggta
     1261 aatacaccgc tttttgcgtc tgcccctgct cggttaccgg cgccactaaa aatgcatcgc
     1321 caaacatata ttgcgagcct aaattaatga cttttgcatc ctgcgggaaa tcaaaccaca
     1381 gcgcccgcat aaagggcgtg cctgtgtcgt aggtaatttt ggcctgggca taaatatagg
     1441 gaattaattg ataacgcagg cggttgtact gggcgagtat ggcctccgcc tgtttgccga
     1501 acgaccaaat ttccgtttgt gcacgcatac cgtgaatacg caacgtgggc gtaaataccc
     1561 cgtactggaa ccagcgggta aataattccg ggtaatcgtg attattgccc accactgcgc
     1621 gggcatcgga aggctctaaa agtgccggtt tggtagcact ggtggtttgc ggcagccact
     1681 gccagccacc tatatcattg ccccagtacg caatgcctga ggcggtcata tttaagccgg
     1741 tgggcacctg gcgcgccaaa gtatcccaac tggggttaat atccgacgac caaaataatc
     1801 cgcccgtgcg ctgggagccc aaataggccg cccgcgccaa aattaaactg cgcacattgg
     1861 gcctatattc gcgcaagccc tcggcaaaac cctccacgtg caccaacgga taaacattgc
     1921 gatagcgatc gccagagccg atgctaaaaa ataatccctc cggtactaaa tccggttcgg
     1981 tttcatccag ccaagggtaa tcaaacccgg cggataaaac gccgtcgcgg gcgtgttgcc
     2041 agaaccattt gcgtgcagcg ggattagtcg aatcaattaa accgccagtg cggtcgttgc
     2101 ggtagggcaa gccgtcctgg gttttaccct ctttatcttt gagaaaataa cccttggcat
     2161 ccagctcatt aaaataccgc gaggcttttt caaagcgcgg ccaaatcgaa ataatggatt
     2221 taatccccat a
//
LOCUS       DAOQGS010000657         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32201_length_2230_cov_3.578851, whole genome shotgun sequence.
ACCESSION   DAOQGS010000657 DAOQGS010000000
VERSION     DAOQGS010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32201_length_2230_cov_3.578851"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..414
                     /locus_tag="PKZ52_17390"
     CDS             142..414
                     /locus_tag="PKZ52_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6471 domain-containing protein"
                     /protein_id="HNG61601.1"
                     /translation="MSKRVLSNTQWRDLVSRLLKSEMSKRKLKYEDLSRALAEEGVIQ
                     TADNLRNKINRGILGADLLVQLLVVLNVKSVERQTLVEIMEDLKGS"
     gene            complement(571..1272)
                     /locus_tag="PKZ52_17395"
     CDS             complement(571..1272)
                     /locus_tag="PKZ52_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61602.1"
                     /translation="MKSVVLGIGLALTCSLCAPLHALTLTVPGTANPWLAGMPDGATA
                     SQDSAPAQAPIMIDLSQLGAGNFLTFTALGATDNGGGLTFGPDGDDSGWVIEHAYGAE
                     NGIASSSLPFLSLTGVFLDDSQPDASATPTPLDYNSLTTSFTSFAAQLKQPFFIGDGL
                     SGNGTGTIQHFLIPTHATRLYLGTADGYGWNNNGGLLMVSVSAVPTPANAWVMVAGLA
                     LFGVMASRKRYITGH"
     gene            complement(1442..>2230)
                     /locus_tag="PKZ52_17400"
     CDS             complement(1442..>2230)
                     /locus_tag="PKZ52_17400"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNG61603.1"
                     /translation="SRLKPKPSTYKLPSKLLLGNWAIAVCALLAVVALGLFSGPSSSS
                     HTHWVSAIGEQKQIRLEDGTQILLNTNSQLEVDFSRDHRLARLVRGEAYFDVHPNPAR
                     PFDVETMQGMVRVLGTHFSVQQQAADTLVTVLEGRVALGAAAAPEQPFKALVELLPNQ
                     QLSLQQAATGSAPRTINAKAALAWRDKQLVAQKQPLVEVVEELERYYPVNIDLAEPAL
                     GAREVTAVIQISALDVTLAALCRPLNLIPQYSPDRTRVTLKPQT"
BASE COUNT          484 a          533 c          680 g          533 t
ORIGIN      
        1 gctccagtgg gtgttcctgc tcgacacagg tggggttaag gtgaataata gctgaaaaat
       61 aatatctggc aagacattat tttattttta tggtaaatat ttttatcctt tgtaaattat
      121 aagttacttt cgggtgcctg aatgtctaag cgagtgctat caaatacgca gtggcgggat
      181 cttgtttcgc gtctgctcaa gtccgaaatg tccaagcgca agcttaagta cgaggattta
      241 agccgcgcct tggctgaaga aggtgttata caaactgccg acaatctacg caacaaaatt
      301 aaccgcggca ttttgggggc tgacctattg gtgcagttgc tggtggtgtt gaatgtgaaa
      361 agtgtggagc ggcagacgtt ggtggagatt atggaggatt tgaaaggcag ctgatggcga
      421 gtttaatagc tctgagcgga atctgttagc cgttgagtgt tatcgcaggt tattgcgcga
      481 cactactgac aaatgagctg tgatatatcc caatctcagt cattgcgacc gggatcgtgg
      541 cgtgcggtta acacgcccta cctgtttaat ttaatgcccg gtgatataac gcttgcgact
      601 ggccatcaca ccaaacagtg ccaaacccgc caccattacc caggcgttgg ccggtgtagg
      661 taccgcactt acggatacca tcagcaagcc gccgttatta ttccagccat agccatcagc
      721 ggtgccgagg tagagccgag tggcgtgggt ggggatgagg aagtgttgga tagtgcccgt
      781 tccattgccg ctcagcccgt caccgatgaa aaacggttgt tttaattggg cggcaaagct
      841 tgtgaagctg gtggttaggc tgttgtagtc cagaggggta ggggtggcgg acgcgtcggg
      901 ttggctgtca tcgagaaaga cacctgttag gcttaaaaac ggcaaggatg agctagctat
      961 accattttca gcgccgtagg cgtgttcgat tacccagccg gaatcatctc catctggccc
     1021 gaatgtcagg ccgccgccat tgtcagtggc acccaaggcg gtaaaggtta aaaagttgcc
     1081 tgcgcccaat tgcgataggt cgatcatgat gggcgcttgg gccggtgcgc tgtcttggga
     1141 ggcagtggcg ccgtcgggca tgccagctaa ccaggggttg gcggtgccgg gtacggttag
     1201 ggtgagggca tgaagggggg cgcaaaggct gcaggtgagg gccaaaccta tacctaaaac
     1261 aacggatttc atcgggggta ccttacgagt ggttatattt tgaccaatgt agaccccagt
     1321 tgaatagctg gcaacaccat ccgtcggctg cacttatccc tgtgtaaaaa ttgcgcagtc
     1381 aattgcaaaa attctattga gcttagcctc cccgattggg ccgggcgcgg cgggcacctg
     1441 gttaggtttg cggctttagg gtgacccggg tgcgatcggg cgaatattgg ggaattaggt
     1501 ttagtggccg gcagagggcg gccaaggtga catccagggc gctgatttga atgaccgctg
     1561 tgacctcccg tgcccccagc gctggctccg ccaggtcgat attgaccggg taatagcgtt
     1621 ctagctcttc tactacttcc accaagggtt gtttttgtgc aactagctgt ttatctcgcc
     1681 aggctagggc ggccttggca ttgatggtgc ggggggcgct gccagtggcg gcctgctgca
     1741 gggatagttg ctgattgggc aatagctcca ccagtgcctt aaatggctgc tccggcgcgg
     1801 cggcggcacc gagggcgacg cgcccttcca gtacggtaac cagtgtgtcg gcagcttgtt
     1861 gctgcacgga aaaatgagtg ccgagcaccc gcaccattcc ttgcatggtc tctacatcga
     1921 agggccgcgc tggattgggg tgcacatcaa aataggcctc accgcgcacc agccgggcta
     1981 gcctgtgatc gcggctgaag tccacttcca gctggctatt ggtattgagc agtatctgtg
     2041 tgccatcttc aaggcgaatc tgtttctgct cgcctatggc gctcacccag tgtgtgtggc
     2101 tgctggatga agggccgcta aacaggccga gcgccaccac ggccagcagt gcgcacaccg
     2161 caatggccca attgcccaaa agcagtttgg atgggagctt ataagtagaa ggcttaggct
     2221 ttaatcggct
//
LOCUS       DAOQGS010000658         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32232_length_2228_cov_5.867464, whole genome shotgun sequence.
ACCESSION   DAOQGS010000658 DAOQGS010000000
VERSION     DAOQGS010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32232_length_2228_cov_5.867464"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKZ52_17405"
     CDS             <1..196
                     /locus_tag="PKZ52_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61604.1"
                     /translation="NKHAAQTNKTQRPCVSPHYEKGWRPSQIQKSPTQSGQARPAEKY
                     AGVAWPLFFVGLNKGRHFIY"
     gene            complement(193..741)
                     /locus_tag="PKZ52_17410"
     CDS             complement(193..741)
                     /locus_tag="PKZ52_17410"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNG61605.1"
                     /translation="MLTRLSPTIYHALHALEQQGTQLALLLIRLLLAVEFGMSGWEKF
                     TGENWFAEIQQQFPWPFNWLPVNLSWTLSTWAELLGAAALVLGLGTRFTAISLGVVTW
                     VATLAVHWPSQWHSLNELAMGYVITDMGAGNFKLPLIYGVLLLPLLTLGAGRISLDYW
                     FRRRFDASFKGSSQQVLLSAKL"
     gene            864..1739
                     /locus_tag="PKZ52_17415"
     CDS             864..1739
                     /locus_tag="PKZ52_17415"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG61606.1"
                     /translation="MEIYQIRYFLAVSETLNFTRAAERCYVSQPALTKSIQKLEELLG
                     GRLFDRTKNSVQLTELGRAMLPNFQQIYAAAQNTKEQARRLLREQRAIVRVGIMCSLD
                     FNRLLPVIADFQVQHPQVLVQMREGTMEMLSEWLDKNDLDLALMSSPYDFPKRFDAHA
                     LFAEDYVVAYAPGHKYAQKCQLTLADLHQEPYCSRLNCEYADYIDNTLDQGGIDLIVV
                     QESPREDWIASMVRAGLGVAFMPQSLAELAHLPFIKVADAHFRRRVQALTLAERPAAP
                     STQALINQLANWPWL"
BASE COUNT          559 a          562 c          551 g          556 t
ORIGIN      
        1 aaataaacat gccgcacaaa ctaataaaac gcaacggcca tgcgtttcac cccattatga
       61 aaaagggtgg cgcccatcgc aaatccaaaa aagcccaacg caaagcggcc aagcacgacc
      121 tgcagaaaaa tacgcaggag tggcttggcc gctctttttt gtggggctca ataagggcag
      181 gcattttatc tattagagtt tggctgagag caaaacctgc tgtgaggaac ccttaaagct
      241 tgcgtcaaat cggcgccgaa accaataatc caggctgatg cgcccggcac ctagggtaag
      301 taatggcagc agcagtaccc cgtaaataag cggcaattta aaattccccg cccccatatc
      361 ggtaatgaca taacccatgg caagttcatt taagctatgc cattgactcg gccagtgcac
      421 cgcaagggtg gccacccaag taaccacgcc tagggatata gccgtaaacc gcgtgccaag
      481 gcccagcacc agcgcagcgg cgccaagtaa ttccgcccag gtggacaggg tccagcttaa
      541 attcaccggc agccaattaa atggccaggg aaattgctgt tgaatttcgg caaaccaatt
      601 ctcgccggta aatttctccc agccagacat gccaaattcc accgccaata ataggcggat
      661 caataacagt gcaagctgtg taccctgttg ctctagggcg tgcagggcgt gataaatagt
      721 cgggcttaat cgtgttaaca taatcggctc ctcgcggtgt aatgtggggc cagcataggc
      781 ctgtaggcgc agcgagtgaa atactgtgtt tttatggcga cataactttt gtctatccaa
      841 cggcggaata taaggggcgt gcggtggaga tttatcagat tcgttatttt ttggcagtca
      901 gtgaaacgct aaatttcacc cgcgccgccg agcgctgtta tgtatcccag cctgcactca
      961 ccaaatcgat tcaaaagtta gaagaattat tgggtgggcg gctgtttgat cgcacgaaaa
     1021 actcagtgca attaaccgag ttgggccggg ccatgttgcc taacttccaa caaatttatg
     1081 ccgccgcaca aaacaccaag gaacaggccc gccgcctatt gcgcgagcag cgcgccattg
     1141 tgcgagtggg gattatgtgc tccttggatt ttaatcgttt gctgccggtc attgccgatt
     1201 ttcaagtgca gcatccccag gtgttggtgc agatgcgcga gggcaccatg gaaatgctct
     1261 ctgaatggtt ggataaaaac gatttggatt tggccttgat gagttccccc tatgattttc
     1321 cgaagcgatt tgatgcccac gcattatttg ccgaggatta tgtggtggcc tatgcccccg
     1381 gccataaata tgcgcaaaaa tgccagttaa ccttggcgga tttacaccaa gaaccctatt
     1441 gctcgcggct taattgtgaa tatgccgatt atattgataa taccttggac caaggtggaa
     1501 tcgatttaat tgtggtgcag gaatcgcccc gtgaggattg gattgcctca atggtgcggg
     1561 cgggtttagg ggtggcgttt atgccgcaaa gtttagcgga gcttgcgcac ttaccgttta
     1621 ttaaagttgc ggatgcacac tttcgccgcc gagtgcaggc actcactttg gccgagcgcc
     1681 cggcggcgcc aagtacccaa gcattaatta atcaattggc taattggcct tggctctagc
     1741 agcttgttga aatactacct tgctagctct ggcggcgtta aatttggcga aaaaatcctc
     1801 atttactgcg tgtaaactcg gcaattgctc ctgcattgct ctacctcctg catccatgca
     1861 gtcgtccggt tttttcgcca aatttgcctt gccagcgcgt cgccgcctac gtatttcaac
     1921 aagctgctaa aacgggtatt gccagattat gccggtgtat ccacagcgct ttgcactggt
     1981 ggcaatactg cggaataagt gtttttctaa ccatgtgtta ccgatctttt taataggcca
     2041 gttaaaaatg ttctgcttaa gtttgcaggt gtgaaaagtg tgtacagccg caaacacttc
     2101 cctgcaggca ctgcccgccg aggacgcttg cattctattg ccgcctctta atcaaaaact
     2161 tcttattaac ccagcggcaa aatagatcgt cctgatctat tttgccctcg ctccgctgac
     2221 acttgcga
//
LOCUS       DAOQGS010000659         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32239_length_2228_cov_3.688449, whole genome shotgun sequence.
ACCESSION   DAOQGS010000659 DAOQGS010000000
VERSION     DAOQGS010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32239_length_2228_cov_3.688449"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="nagZ"
                     /locus_tag="PKZ52_17420"
     CDS             complement(<1..1000)
                     /gene="nagZ"
                     /locus_tag="PKZ52_17420"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNG61607.1"
                     /translation="MPLNKTGPVILDVLSFALNDEEKEVLCHPQVGGVILFGRNIDSA
                     KQVADLTRHIRSTNPNLLLCVDQEGGRVQRLKNGFTRLPPMGHLGQLYSSEPALALDI
                     SEQLGWLMASEVLGVGIDLSFAPVLDVDSHFSQVIGDRAFSPVPMQVELLGGAFMAGM
                     QRAGMANCGKHFPGHGSVKADSHLELPVDARPMQAIEEHDLRPFRALLPKLAALMPAH
                     IIFPAVDPNPVGFSAAWLQGILRKQMGFNGVIISDDLSMAGAAFAGDYPARAQMALEA
                     GCDGILVCNQPAKAHQVLEFLEGINRKTDGRLQTLQSQSALPLALLHAEAPWHQANE"
     gene            1342..1944
                     /gene="lexA"
                     /locus_tag="PKZ52_17425"
     CDS             1342..1944
                     /gene="lexA"
                     /locus_tag="PKZ52_17425"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNG61608.1"
                     /translation="MLKLTSRQQQIFELIKDQIDTTGYPPTRAEIAQMLGFKSANAAE
                     EHIKALARKGVIEVVEGASRGIRLPDHERGIPIVGRVAAGNPILATQHIEEYCPVPES
                     FFHPRADYFLRVSGMSMKDAGILDGDLLAVHATNQVHNNDIIVARIEDEVTVKRFRRE
                     RNRSVVELWPENPEFNVIQVDLRDRNFSIEGLSVGVIRRG"
     gene            1948..>2228
                     /locus_tag="PKZ52_17430"
     CDS             1948..>2228
                     /locus_tag="PKZ52_17430"
                     /inference="COORDINATES: protein motif:HMM:NF015784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulA-like leucine-rich domain-containing
                     protein"
                     /protein_id="HNG61609.1"
                     /translation="MATQTAAKTQSNIGITEVVLPGDEESLALILPMLAHLSRTCTDR
                     WFTWIAPKGVHREMLVDYGFDLSKLRLVHVKNAEEALWVCWDALVAGNS"
BASE COUNT          549 a          606 c          563 g          510 t
ORIGIN      
        1 attcattcgc ctgatgccag ggcgcttcag cgtgtaacag ggccaagggt aaggcacttt
       61 gactttggag ggtttgcagc cgcccgtcag ttttgcgatt aataccctct aaaaattcca
      121 acacctggtg ggctttagcc ggttggttac acaccaaaat accatcgcaa ccggcctcca
      181 aggccatttg cgcccgtgca gggtaatcac cggcaaaggc cgctcccgcc atggataaat
      241 catcgctaat aatgacgcca ttaaaaccca tctgcttgcg caaaatgccc tgcaaccatg
      301 cggccgaaaa ccccaccggg ttaggatcaa ccgccggaaa aataatatgc gcgggcataa
      361 gcgccgcaag ttttggcagc agcgcgcgaa atgggcgtag gtcgtgttct tcaatggcct
      421 gcatgggcct tgcgtccacg ggtagttcca agtggctatc cgcttttacg ctgccgtggc
      481 cagggaagtg tttgccgcaa ttggccatac ccgcccgctg cattcccgcc ataaatgccc
      541 cccccagcag ttcaacctgc atgggcacag ggctaaaggc gcgatcgcca atcacttggc
      601 tgaaatggct atccacatcc aataccgggg caaagctcag atcaataccc acccccagta
      661 cttcgctggc cattaaccaa ccgagttgtt cgctaatatc gagggccaag gcaggctcac
      721 tgctataaag ctgccctagg tggcccatgg ggggcaggcg ggtaaaacca ttttttagcc
      781 gctgcacccg gcccccttcc tgatccacac agagcagtaa attggggtta gtgctgcgga
      841 tgtgccgagt taaatcagcc acttgtttag ccgaatctat attgcggcca aataaaatca
      901 caccccccac ttgggggtgg cacaaaacct ctttctcctc atcgttcaat gcaaacgaga
      961 gcacatcaag aataactggg ccggttttgt ttaggggcat ataccgtcac ttacaggtaa
     1021 tggatggagc ccggcatgat tgccaggccc agcacgcttg tcaattgcgc ctggggctca
     1081 tctgcccggc aaggcgccac taataagccg tgcctgcacc cttgttatgg catcactcca
     1141 agatccaggg ccagccctta ggcctaatag attcagtcca ggcaaaccgg cgctttattc
     1201 tgccgagtgg atattgccgt tgccgtatag aggtgggtga aatggcgaac acaaacccaa
     1261 agcaagaaaa tacttgcacg gcataaacta ctgtatataa tcccaagtac tgtataagta
     1321 accatgcctg tggggcgcgc aatgctcaag cttacttctc gccagcaaca aatttttgaa
     1381 ctcattaaag atcaaatcga caccacgggc tacccaccca ctcgcgctga aattgcccaa
     1441 atgctcggct ttaagtctgc caatgccgca gaagaacaca ttaaagccct cgcccgcaaa
     1501 ggcgtcatcg aggtggtgga aggtgcttcg cggggtattc gcctgcccga tcacgagcgc
     1561 ggcatcccca ttgttggccg agttgctgcg ggcaatccca ttctggccac ccaacatatt
     1621 gaagaatatt gccccgtgcc cgagtcgttt tttcaccccc gcgcagatta tttcctgcgg
     1681 gtatccggta tgagtatgaa agatgccggt attttggatg gcgatttact ggcggtgcat
     1741 gcaaccaacc aagtgcacaa taacgacatc atcgttgccc gtattgaaga tgaagttacc
     1801 gttaagcggt ttcggcggga gcgcaaccgc tcggtagttg agctgtggcc agagaatcca
     1861 gaatttaatg ttattcaagt tgatttaagg gatcgcaatt tctccatcga agggttgagc
     1921 gtcggcgtaa ttcgtagggg gtgatttatg gcaacacaaa cagcagcaaa aactcagtct
     1981 aatatcggca ttactgaagt tgttttaccg ggtgatgaag aatccctggc attaatttta
     2041 cccatgctgg cccatttaag ccgcacctgt accgaccgtt ggtttacctg gattgcaccc
     2101 aagggcgtac accgggagat gttagtggac tacggttttg atctttctaa actgcgctta
     2161 gtacatgtta aaaatgccga agaagccctc tgggtatgtt gggatgcgct agtggcgggc
     2221 aacagcca
//
LOCUS       DAOQGS010000660         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32340_length_2224_cov_5.266482, whole genome shotgun sequence.
ACCESSION   DAOQGS010000660 DAOQGS010000000
VERSION     DAOQGS010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32340_length_2224_cov_5.266482"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKZ52_17435"
     CDS             <1..338
                     /locus_tag="PKZ52_17435"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNG61610.1"
                     /translation="TYHLTPGQCTQVEPCETQAYVDWVNRQGLCGYADWRLPSIRELV
                     SIAVISSEIPAFDKNYFADTIKPRFFTSQTYAAEPSRAWYVYFSDGSVSSTGKSDASF
                     VRLVRGGQP"
     gene            335..865
                     /locus_tag="PKZ52_17440"
     CDS             335..865
                     /locus_tag="PKZ52_17440"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNG61611.1"
                     /translation="MSVKKVRYILGWVALAWGCAAWAECNSLLKPSYDRSKFVIESKG
                     VVVDLTTGLMWQRCPMGYEWQNSTCVLPVGAVTVFSWEQALEQAAKLKNFAGFSDWRI
                     PNKNELGSIVDYACAQPALDTSLFPETQPSGYWTNSPNTFTPARAWAVNFAYGDHMSS
                     VRTDLLGVRLVREIPR"
     gene            1211..1663
                     /gene="rpsF"
                     /locus_tag="PKZ52_17445"
     CDS             1211..1663
                     /gene="rpsF"
                     /locus_tag="PKZ52_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNG61612.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMVERYKSSITTTGGAIHRFEDWGR
                     RQLAYSINKIHKAHYILMNVECGEAVLDELTTTFRYNDAILRHLVIRTDEAITEESPI
                     MKAEKESRERKARAERRQEEASARAEAERADEDESDEDLDDSDADNEE"
     gene            1669..1896
                     /gene="rpsR"
                     /locus_tag="PKZ52_17450"
     CDS             1669..1896
                     /gene="rpsR"
                     /locus_tag="PKZ52_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNG61613.1"
                     /translation="MSRFFRRRKFCRFTAEGVKQIDYKDLETLKAYVSETGKIVPSRI
                     TGTKAKYQRQLSTAIKRSRYIALMPYTDSHE"
     gene            1909..>2224
                     /gene="rplI"
                     /locus_tag="PKZ52_17455"
     CDS             1909..>2224
                     /gene="rplI"
                     /locus_tag="PKZ52_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNG61614.1"
                     /translation="MDVILLEKVGRLGSVGDKVSVKPGYGRNYLLPKGKAITATASNV
                     AAFEARRAELEKSAAERKQEAETRAAALAELVVTIKANAGDEGKLFGSVGARDIAQAI
                     SAA"
BASE COUNT          526 a          556 c          572 g          570 t
ORIGIN      
        1 ttacctatca tttgacacct gggcaatgca cgcaagtgga accctgcgaa actcaggctt
       61 atgtggattg ggttaatcgg caaggattat gtggttacgc agattggcgt ttgcccagca
      121 tccgtgaatt ggtttctatt gcggttatat ccagtgaaat tcccgctttt gataaaaatt
      181 attttgccga tacgatcaag ccgcgctttt tcacctccca aacctacgcg gcagaaccct
      241 cccgcgcctg gtatgtatat tttagtgatg gcagcgtgag ctcaacgggt aaaagcgatg
      301 cgtcgtttgt gcgtttggta agaggaggtc agccgtgagc gtaaaaaaag tgcgttatat
      361 actgggctgg gtggcgctgg cttgggggtg tgctgcttgg gcagagtgta attcgctgtt
      421 aaaacccagc tacgaccgca gcaagttcgt cattgaatcc aagggtgtag tggtggatct
      481 aaccaccggc ctcatgtggc agcgctgccc catgggctac gagtggcaaa actcaacctg
      541 cgttttacct gtcggagccg taacggtgtt ttcctgggag caggcccttg agcaggcggc
      601 caaattaaaa aactttgccg gttttagcga ttggcgcata cccaataaaa atgagttggg
      661 atcaattgtt gattacgcct gcgctcaacc ggccctagat accagcctgt ttcccgaaac
      721 ccagccctca gggtattgga ctaattcgcc caatacgttt acacccgcgc gcgcctgggc
      781 ggttaacttt gcctatgggg accatatgag ttctgtccgc actgatttat tgggtgtgcg
      841 cttggtgcga gaaattcccc ggtaaaggcg gcgctcaggg tgctttttga tgcggctaag
      901 ccccgagtaa agcgcgtttt actagggttt tttattgtgt gcctagcggc ttaaatgaac
      961 gctcatttat tgttgccagc ctttgctgat aactctattt ctatccttat ttctcttctt
     1021 tgtcggccac cttatcggct ggagcactaa tgagttgccg cttatgccaa cggcggcagc
     1081 aaatttgcct tgcttccctg tgccttatcc cctactatgc gcgccccaaa tctcaccggg
     1141 tggtttcacc ctggtggttc tccttgtctt accgcgtggc ggaagactat taacccgtaa
     1201 ggagctaatc atgcgtcatt acgaaattgt atttctggta catccagatc aaagcgaaca
     1261 agtccccgcg atggtggagc gctataaaag ctctatcacc actaccggtg gtgctattca
     1321 ccgctttgaa gactggggcc gccgtcaact ggcctattcc atcaacaaaa ttcacaaagc
     1381 tcactacatt ctgatgaatg ttgagtgcgg tgaagctgtg ctggatgagt tgaccaccac
     1441 tttccgttac aacgacgcta ttttgcgtca cttggttatc cgtactgatg aagcgattac
     1501 tgaagagtcg cccatcatga aagcggaaaa agaaagccgt gagcgtaaag cccgcgccga
     1561 acgccgccaa gaagaagcct cagcccgcgc tgaagctgaa cgtgctgatg aagacgagtc
     1621 cgatgaagac ttggacgatt cagacgctga taacgaggag taattgccat gtcacgtttt
     1681 ttccgtcgtc gcaagttttg ccgttttacc gctgaaggcg taaaacaaat cgattacaaa
     1741 gatctggaaa ccttgaaagc ctacgtatct gagaccggta aaatcgttcc cagccgtatc
     1801 actggcacta aggctaaata ccagcgccaa ttgtctaccg ccatcaagcg ttctcgttac
     1861 atcgctttga tgccatacac tgacagccac gaataagggg taggccagat ggacgtaatt
     1921 ctgttagaaa aagttggccg tttaggctcc gttggcgaca aggtcagtgt aaaacctggc
     1981 tatggtcgca actatttatt gcccaaaggc aaggcgatca ctgcaactgc gtctaacgta
     2041 gctgcctttg aagcccgccg cgccgagttg gaaaaatcag ctgctgagcg caagcaagaa
     2101 gctgaaaccc gtgcggccgc attggctgaa ttggttgtta ccatcaaggc taatgccggt
     2161 gatgaaggta aactcttcgg atccgtcggt gcccgcgata tcgcccaagc cattagtgct
     2221 gcag
//
LOCUS       DAOQGS010000661         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32417_length_2221_cov_6.098338, whole genome shotgun sequence.
ACCESSION   DAOQGS010000661 DAOQGS010000000
VERSION     DAOQGS010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32417_length_2221_cov_6.098338"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKZ52_17460"
     CDS             <1..389
                     /locus_tag="PKZ52_17460"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNG61615.1"
                     /translation="SMLVPIVCVGESLAERQSGKALQVIERQLSAVIDHCGVERLARA
                     VVAYEPLWAVGTGVTAAPEQAQEVHAHIRGMLGSVGMLTRIVYGGSVKAGNAKALFAQ
                     PDIDGALVGAASLIAKEFLDICQAAE"
     gene            448..846
                     /gene="secG"
                     /locus_tag="PKZ52_17465"
     CDS             448..846
                     /gene="secG"
                     /locus_tag="PKZ52_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNG61616.1"
                     /translation="METFILVLHVVIALAIIGLIMLQQGKGAEMGASFGSGSSQTIFG
                     AVGSGNLFSRLTAILVAVFFITSSSLAVMSKHKSKIDDHFLAPATETAPATAAEVKKE
                     AAPEALESPKAEEKPADAKPADGADALENK"
     gene            851..935
                     /locus_tag="PKZ52_17470"
     tRNA            851..935
                     /locus_tag="PKZ52_17470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:885..887,aa:Leu,seq:gag)
     gene            1092..>2221
                     /locus_tag="PKZ52_17475"
     CDS             1092..>2221
                     /locus_tag="PKZ52_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HNG61617.1"
                     /translation="MAGRDKLTEKAVKLAKPKEKSYRLADGGGMYLEVMPNGSKYWRL
                     KYRLGGKEKRLALGVYPAVTLASARIKYRAAKEGLAEGQDPSQTKKHDKLARQAATQN
                     TFATIGQELLTKREQEGAAPVTLAKIKWLLDKKLCPYIGGICVAEITPVQLLTALRKI
                     EAEGLHETASRAKRVASQVIRYAIATGRAERDICQDLNGALVAPKVTHRAAITKPEEL
                     APLLAAIDSYNGTPEVTAALKLTPMLFQRPGEIRHMEWAEINWEAAYWEIPAEKMKMR
                     LPHIVPLCSQAITILKNLQPLTGQGRYVFPSARRGGRPMSEAAVLAALRTLGYSREQV
                     TPHGFRATARTLLDEVLKYRVDFIEQQLAHAVKDANGRAYNRT"
BASE COUNT          554 a          638 c          589 g          440 t
ORIGIN      
        1 cctctatgct ggtacccatt gtgtgtgtcg gtgagtcact cgccgagcgg cagtcgggaa
       61 aggctctgca agtgatcgag cggcagctgt ctgcggtcat tgaccactgc ggggttgagc
      121 gcctagccag agcggtcgtt gcctatgagc ctctctgggc tgtcggcaca ggcgtaaccg
      181 ccgcgccgga gcaagctcaa gaagtgcacg cgcacattcg cggcatgctt ggcagcgtag
      241 gtatgctaac ccggattgtg tacgggggca gtgtaaaagc tggcaatgct aaggcgcttt
      301 ttgcgcagcc agatatagac ggtgctttag ttggtgcggc gtcgctaata gcaaaagaat
      361 ttctagacat atgccaggca gcagaataaa agcgcttgac catataggtg ttaactggtt
      421 gttaattgtg tgtaacgaga gagattgatg gaaacgttta ttctggtact gcatgttgtc
      481 atagcgctgg ccattatcgg cttgatcatg ctgcagcagg gtaaaggcgc cgagatggga
      541 gcctcttttg gctccggctc atcgcaaacc atcttcggcg cagtcggctc cggcaacctc
      601 ttttctcgct tgaccgccat cctggttgcc gtgtttttta tcaccagctc aagcttggcg
      661 gttatgtcaa aacacaagtc caagattgat gatcactttt tagcacctgc caccgaaact
      721 gctcctgcca cagcggcaga agtgaaaaaa gaggcagctc ccgaagcatt ggaatctccc
      781 aaagctgaag aaaaacccgc tgatgccaag cctgccgacg gcgctgacgc actggagaac
      841 aagtaattta gcccaagtgg tggaattggt agacacgcta ccttgaggtg gtagtggcaa
      901 tagctgtgcc ggttcgagtc cggccttggg caccatttta aaatcctgca acatccaata
      961 cagccttcaa acccgcgtaa atactgggtt tgaagcgctt caagctccaa cgacgtccaa
     1021 cccaatccat tgaaagccag aacttctggg ggcataattg ggggcacagg aacgcaaggt
     1081 tgggggcaca aatggcaggc agagataagc tcaccgaaaa ggccgtcaag ctggcgaagc
     1141 ctaaggaaaa atcctaccgc ttagccgacg gcggcggcat gtacctagag gttatgccca
     1201 atggctccaa gtactggcgg ctaaagtacc gtttaggcgg caaagaaaag cgcctcgcct
     1261 tgggggtgta ccccgccgtt accctggcca gcgcccgcat caaataccgc gccgctaaag
     1321 aaggcctcgc cgaaggccaa gatccaagcc aaaccaaaaa acacgacaaa ctcgcccgcc
     1381 aagccgccac ccaaaacacc tttgccacca ttggccaaga gctgcttacc aagcgcgaac
     1441 aggaaggcgc agcgcccgta accctggcaa agataaagtg gctactggat aaaaaattgt
     1501 gcccctacat tgggggcatt tgcgtcgctg aaattacccc cgttcaactg cttactgcac
     1561 tgcgcaaaat agaagccgaa ggcctgcacg aaaccgccag ccgcgccaaa cgtgttgcca
     1621 gccaggttat ccgctacgcc atagccaccg gccgggccga gcgcgacatc tgccaagact
     1681 taaacggcgc ccttgttgcc cctaaagtca cccaccgcgc cgccattact aagcctgaag
     1741 aactagcgcc actgcttgcc gccatagata gttacaacgg cacacccgaa gtcaccgccg
     1801 ctttaaaact cacccccatg ttatttcagc gccccggcga gatacgccat atggaatggg
     1861 ccgaaattaa ttgggaggcg gcctattggg aaattccagc ggaaaaaatg aaaatgcgcc
     1921 tgccgcatat tgtgcccctc tgcagtcaag caattaccat tttaaaaaac ctgcagccat
     1981 taaccggcca gggccgctat gtgttcccca gcgcccgcag aggcggccgc cctatgtcgg
     2041 aagccgccgt gctcgctgcc ctgcgcaccc tgggttacag ccgcgaacaa gtgacgcccc
     2101 acggctttag agccaccgcc cgcaccttgc ttgatgaggt tttaaagtac cgcgtggatt
     2161 tcatagagca acaacttgcc catgcagtta aagatgccaa tgggcgcgcc tataaccgca
     2221 c
//
LOCUS       DAOQGS010000662         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32844_length_2206_cov_5.551371, whole genome shotgun sequence.
ACCESSION   DAOQGS010000662 DAOQGS010000000
VERSION     DAOQGS010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32844_length_2206_cov_5.551371"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..984)
                     /locus_tag="PKZ52_17480"
     CDS             complement(166..984)
                     /locus_tag="PKZ52_17480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNG61618.1"
                     /translation="MSDAFYSSATDVGLFRDNNEDAIVCDGQLNLFLVADGMGGHEAG
                     EVASAIAGDIVAKAIAKGEDLSEAIQQSHRAILHAAEIGQGHKGMGSTIVALLSQGNQ
                     YQIAWVGDSRAYLWRNQPEGKSLQQLTVDHSYVQMLYQHGAITAAEMARHPEKNIITQ
                     CLGSTDLARVDVDEVWGHWQKNDWILLCSDGLTDAVNDQMLCDVLSLHQEPQAAVDAL
                     VKTALNQGGRDNISVVLVAKPPGNWKQWLPGRFNRAKIQGFFKRWRSRSADVAH"
     gene            1252..2145
                     /locus_tag="PKZ52_17485"
     CDS             1252..2145
                     /locus_tag="PKZ52_17485"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNG61619.1"
                     /translation="MLQLQFLNFAKPPMWLVESTYHVGSSAQCNCVIDLPGVLPQHAQ
                     LLIDNDQAQLTPIAAHTAVNGTLINAPTLIGHGDTLELGGALIAVVDPKLARKANLQP
                     AAAPDADVTQVVAVARSAWYLQPMSTALEPNVVEINGKMVLGRAKECDVRLNFAHLSR
                     RHAVLTLTPQGLELEDLGSSNGTFVNDVKVRRVMLKNGDVLSFDNLRFRIVGPEEETD
                     KTQLRSPSAAEENPTYLRPEQTPVGAPMAAAMRQRAAPQVIRPSVAAESDDSRQGRMF
                     LLVGTLAVVLLFATLIFFLLH"
BASE COUNT          499 a          595 c          587 g          525 t
ORIGIN      
        1 actaaggcgc tgaatatgcg cgcttttttc taaaaattgg gcgaaagcag tagtcgacac
       61 tacggtgagt ccaattttta gaaaaaagcg cgcaggttca agtattaggc ggccgtagta
      121 gagttcaact gtggttttta ggtttaacgg cggggcggtg aagcgctagt gcgctacatc
      181 ggctgagcgg ctccgccagc gtttaaaaaa cccttgaatt ttggcccggt taaagcgacc
      241 gggcagccat tgcttccaat tgccgggcgg cttggccact agcaccaccg atatgttgtc
      301 ccggccgccc tggttcaaag cggtttttac cagggcatcc accgccgctt ggggctcttg
      361 gtgcaggctt aacacatcgc acagcatctg gtcgtttacc gcatcggtga ggccatcgct
      421 acacagtaaa atccaatcat tcttctgcca gtgcccccag acttcatcta catctacccg
      481 cgctaaatcg gtggagccca agcactgggt aatgatgttt ttttccgggt gacgggccat
      541 ttcggcggcg gtaatagcgc cgtgttgata gagcatctgc acataggagt gatccaccgt
      601 gagttgctgc aggcttttac cctccggctg attgcgccaa aggtaggcgc ggctatcgcc
      661 cacccaggcg atttgatact gattgccctg gctcaacaag gccacaatgg tggagcccat
      721 gcccttgtgg ccctggccta tttccgctgc gtgcagtatg gcgcggtggg attgttgaat
      781 ggcttcactt aagtcttcgc ccttggcgat tgccttggcc actatatccc cggctatagc
      841 gctggccacc tcaccggctt catgcccccc catgccatcg gcgacgagaa acaagttcag
      901 ctgtccatca caaactatgg catcttcatt attatcgcgg aacaaaccaa catcggtggc
      961 actgctgtaa aaggcgtcag acatgaaaca aaacggttcc taaagcttaa ggggcgagac
     1021 taacctaaaa acacctaatt gtcttgattg cacaaaagct tagcagttgg aatgtgcagg
     1081 gctgcgggtt ttgatgcggc agtggggctt tcgcttgctt aacgggcgca ctactgatga
     1141 gtgtgcggga tattttgctg tgctgagtcc tgtttggtat aaagtcgctc cccggataaa
     1201 cacacaatcc gtatacttcc tgcgaattcc ccacttctgg accctgtcga catgctgcaa
     1261 ttgcaatttc ttaattttgc caaaccaccc atgtggttag tggaatctac ataccatgta
     1321 ggttcatcgg cacagtgcaa ctgcgttatc gacctgcccg gtgtactgcc gcagcacgcc
     1381 cagttgttga ttgataacga tcaagcccaa ctgaccccaa tcgctgctca taccgcggtg
     1441 aacggcacgc ttataaatgc gccgacccta attggccatg gcgatacgct ggagctgggg
     1501 ggcgcgttaa ttgcggtggt ggaccctaag cttgcgcgca aagccaacct gcaacccgct
     1561 gcggcacccg atgccgatgt cacacaagtt gtcgcggtag cccgctccgc ctggtatcta
     1621 cagcccatga gcacggcctt agagcccaat gtggtggaga tcaacggcaa gatggtgctg
     1681 gggcgcgcaa aagaatgcga cgtacgcttg aacttcgccc acttatctag gcgccatgct
     1741 gtattaaccc taaccccgca aggcttggaa ttagaagact taggctccag caatggcacg
     1801 tttgtaaacg acgtaaaagt tcgccgcgtg atgttaaaaa atggcgatgt attaagtttt
     1861 gataatttgc gttttcgaat cgtcggcccg gaagaagaaa ccgataaaac ccagttgcgc
     1921 tcgccaagcg cagcagagga aaaccccacc tatttacgcc cagaacagac tccggtgggc
     1981 gcccccatgg cggcggccat gcgccagcgg gccgcgcccc aagtcattcg gccgagtgtc
     2041 gctgccgaat ccgacgactc tcggcagggg cgcatgtttt tattggtggg cactttggca
     2101 gtggttttat tatttgccac gctgattttc ttcttgctgc attaatttag agttaaggaa
     2161 cctctgaaaa atgtaggcgg cgaagcacta gctaggcgaa tttggc
//
LOCUS       DAOQGS010000663         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32894_length_2204_cov_6.009772, whole genome shotgun sequence.
ACCESSION   DAOQGS010000663 DAOQGS010000000
VERSION     DAOQGS010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32894_length_2204_cov_6.009772"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..838)
                     /locus_tag="PKZ52_17490"
     CDS             complement(239..838)
                     /locus_tag="PKZ52_17490"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNG61620.1"
                     /translation="MLQANHHFDLLAAQIFAKLQEHQWRTFGVTSARDGEGKSTVAHR
                     LALSMARERKHTVMLVDLNVFNPQVHNLFKRKNDMHLGKVLSGAVEFGEVIQGTDVER
                     LKIVANSQSGMAGADLMSSFTFERLIAEISQRYADRICIFDMPSYLESNAALTVTPFL
                     DSLLLVVRDGVTQTKDLQAVSRLMGERKILGLVLNDVAS"
     gene            complement(847..>2204)
                     /locus_tag="PKZ52_17495"
     CDS             complement(847..>2204)
                     /locus_tag="PKZ52_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61621.1"
                     /translation="ADVVDPRSGRPVEATIAFSIKYHSKSADTAQKVAGEITSLFLNE
                     NLKNRKEMASLTEEFLGKSLEEERDKLKALEARLAQFKQEHAKELPEFNQFTISTLER
                     TETELRERTARLDSLQERRIYLKGELARLGASRIVTGGDLNGLTPEDRLRYLYNLSLQ
                     LKTQYGANHPDVIRTNNAINELLASGAQLRDKSFLKDIKQRREQELKLLESKYSPSHP
                     DVAKLRQQIEQLGKLETTAVSSNFESSNNPDYIQLAAQLQAIEQNLISEQAIIGGLQE
                     KIQTLQASLSQSPKVEQDYRALMRELENANRNFLEYQAKQTQAKLATQLEQEQKGERF
                     TLIEPPMRPEEPVSPNRPLLLVLAFVLSAAAAIGCVLGLEKIDGTIRSEGALVRLSGI
                     APLVVIPHMAAHQIEKRALLSWQPVAVSAGIILVVFTLVHFLYKPLDVVYYILIRKFL
                     G"
BASE COUNT          572 a          560 c          473 g          599 t
ORIGIN      
        1 agagcaatgc aggagcaatt gccgagttta cacgcagtaa atgagcattt ttttgccaaa
       61 ttcaacgacg ctagggcgag tgaggtaatt tttcagaggt tccctagcag cttgttgcaa
      121 taagtcgtta gccaggcacg cccttgtgac gtaaaaaagc cagctttcgc tggctttttt
      181 gtttaaggta tttgtactgc cttataaggc ttgatcaatc gaatcaaaaa ccgcgccatt
      241 aagacgctac atcatttaaa accaagccta atatcttgcg ctcccccatt aaacgggaca
      301 ctgcctgcag gtcttttgtc tgcgtgacac catcgcgcac aaccaaaagc aaactatcta
      361 aaaatggtgt taccgtaagt gccgcattgg attctaaata agatggcata tcaaaaatac
      421 agatacgatc agcatagcgc tggctaattt cggcaattaa acgctcaaag gtaaacgaac
      481 tcattaaatc ggcacccgcc atgcccgatt gactattggc aacaattttt aaccgctcaa
      541 catccgtccc ctgaattacc tcgccaaact ccacagcacc gctgagcact ttacctaagt
      601 gcatgtcatt tttacgctta aaaagattgt gtacctgcgg attaaataca ttgaggtcaa
      661 ccaacatcac tgtatgcttg cgctccctgg ccatacttag cgcaagtcgg tgggcgacgg
      721 ttgatttgcc ttcgccatca cgggcgctgg tcacgccaaa ggtgcgccat tgatgttcct
      781 gcaatttagc aaaaatttgc gctgctaaca gatcaaaatg gtgattggct tgaagcataa
      841 aagtcactaa cctaaaaatt ttcggattag aatgtaatac accacatcca gtggcttata
      901 taaaaaatgt accagggtaa aaaccaccag aataatacct gcggagacag cgaccggttg
      961 ccaagacaac aacgcacgct tctcaatttg atgcgccgcc atatggggga ttactaccaa
     1021 gggcgctatt ccacttaaac gcaccaaagc gccctccgag cgaatagtac cgtctatttt
     1081 ttctaagccg agcacacagc ctatagcggc ggcggcgctt agaacaaagg ctagtactaa
     1141 taataaaggc cggttggggc tcacgggctc ttcaggtcgc atgggcggtt caattagagt
     1201 aaagcgctcg cctttttgct cctgttccag ctgggtagcc aacttagctt gcgtctgttt
     1261 ggcctggtat tccaagaaat tgcggttggc attttctaat tcgcgcataa gtgctcgata
     1321 atcctgctcc actttaggcg actggctcaa agacgcctgc agggtttgaa ttttttcctg
     1381 cagcccgccg ataatagcct gctccgagat aagattctgt tcgattgcct gcagctgcgc
     1441 cgccaactga atataatccg gattgttaga actctcgaaa ttactggata ctgcagtagt
     1501 ttccagcttg cccagctgct caatttgctg gcgcagcttc gctacgtcgg gatgactagg
     1561 ggaatatttg ctctccagca gtttaagctc ctgctcgcgg cgctgtttta tatcttttaa
     1621 aaaactttta tcccgcaact gtgcgccgct tgccaataat tcattaatcg cattattggt
     1681 gcgaatcacg tccggatgat tggcgccata ttgggtttta agctgcaaag acagattata
     1741 caaataacgc agtctatctt ccggtgttaa accgttcaaa tcaccacccg tcacaatacg
     1801 gctagccccc aaacgcgcca gctcgccttt taaataaatt ctgcgctcct gcagggaatc
     1861 taaacgcgcc gtacgctcgc gcaactcagt ttcggtgcgc tctagtgttg aaatcgtaaa
     1921 ctgattgaat tcaggcagtt ctttggcgtg ctcttgttta aattgcgcca gcctagcttc
     1981 cagagccttt agtttatccc gctcctcttc taggcttttg cccaaaaact cctcggttaa
     2041 gcttgccatc tctttacgat tttttaggtt ttcatttaaa aataaactgg taatttcacc
     2101 ggccactttc tgcgctgtat cagcactttt gctgtggtat ttaattgaaa aagcaatcgt
     2161 cgcttcaacc ggcctgccac tgcgggggtc taccacatcg gcgc
//
LOCUS       DAOQGS010000664         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_32924_length_2203_cov_4.756052, whole genome shotgun sequence.
ACCESSION   DAOQGS010000664 DAOQGS010000000
VERSION     DAOQGS010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32924_length_2203_cov_4.756052"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PKZ52_17500"
     CDS             complement(<1..1637)
                     /locus_tag="PKZ52_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG61622.1"
                     /translation="MPKLSQTPGSLPLFIAAFLNAFVDLGHKIIVQNTVFKLHDGPQQ
                     VALTALVNAMMLIPFIVLLSPSGFLSDQYRKTRVMQVAAWVAVGLTLLITLFYYLGWF
                     WPAFSMTLLLAAQATIYSPAKYGYLKELFGKERLGQANGVMSAVSMIAILGGIFAYSI
                     AFEILFPKGATTEAEVLRAIAPVGWLLVLNSLIEVAMLYRLPAQAPVVKTESFAFGRF
                     FSGQSFKDDLAPLKHSRSIRLAIIGLATFWGVGQVMLAAFPAFVKEELGLENTIAVQG
                     ILACTGIGIALGSYVAGRVSKNYIDTGLLPMGALGIAIGLGVLTFLHSFSAFTLVFLL
                     VGFCGGLFTVPLNALIQFNAKSEELGRTLAANNWVQNVVMLSFLGLTFLFSLWNLSSK
                     TLLQLIAWVALIGCCYTVWQLPQSFMRAALGYILTRRYKITVQGIKNIPPQGGVLMLG
                     NHVSWIDWGILQLASPRPVRFVMIRSIYSRWFLKWFFDLFGCIPIEPGARSRKALDDV
                     ATALNNGEVVCLFPEGSISRTGHLGEFRKGFERACSQV"
BASE COUNT          624 a          542 c          555 g          482 t
ORIGIN      
        1 acctggctgc aggcccgctc aaaccccttg cgaaattccc ctaaatggcc ggtgcggcta
       61 atggagcctt ctggaaataa acagactact tcgccgttat ttaaagccgt tgctacatcg
      121 tccagcgcct tgcggctgcg ggcgccaggt tctatgggaa tacagccgaa taaatcaaaa
      181 aaccatttta aaaaccaacg cgaataaatg ctgcgaatca tcacaaaacg cacggggcga
      241 gggcttgcca attgcaaaat accccagtcg atccagctga catggttgcc cagcattaat
      301 acgcccccct ggggcgggat atttttaatg ccttgcacgg tgattttata gcggcgggtt
      361 aaaatatagc ctagcgcagc gcgcataaaa ctctgcggca attgccacac ggtgtaacaa
      421 cagccgatta aggccaccca ggcaattaat tgcagcaggg ttttactgct taaattccac
      481 aggctaaaca aaaaagtgag gcctaaaaaa ctcagcatca ccacattttg cacccagtta
      541 ttggcggcca gggtacggcc taattcctcg cttttggcat taaattgaat gagcgcatta
      601 agaggcacag taaacaggcc gccgcaaaaa cccaccaata aaaataccag ggtaaatgcg
      661 ctaaaactgt gtaaaaacgt cagcacgcca aggcctatgg ctatgcctaa ggcacccatg
      721 ggcaacaggc cggtgtcgat ataatttttc gatacgcgcc cggccacgta agagcccagc
      781 gcaatgccga tgcccgtgca ggctaaaata ccctgcacgg cgatggtgtt ctccaggcct
      841 aattcttctt ttacaaaggc ggggaaggcc gccagcatga cttggccgac cccccaaaaa
      901 gtggctaagc cgataatagc gagccgaata ctgcggctgt gttttaacgg tgctaaatca
      961 tccttaaagg attgaccgga aaaaaagcgc ccaaaggcga aggattcggt ttttactact
     1021 ggtgcttgag ccggtaaacg gtacaacatg gccacttcaa ttaatgaatt taatactaac
     1081 agccagccca ccggcgcaat ggcgcgcaat acttcggcct cggtggtagc gcctttggga
     1141 aagagaattt caaaagcgat ggaataggca aatataccgc ctaaaatggc gatcatggac
     1201 accgccgaca taaccccatt ggcctggccc aaacgctctt tgccgaataa ttcttttaaa
     1261 tagccgtatt tagcgggcga ataaatagtg gcctgggcgg cgagcagcag ggtcatacta
     1321 aaggcgggcc agaaccagcc taaataataa aacagcgtaa ttaatagagt gaggcccacg
     1381 gctacccagg cggccacctg catcacgcgg gttttacggt actgatcaga taaaaacccc
     1441 gatgggctta acagcacaat aaaggggatc agcatcatgg cattgaccag agcggtcaat
     1501 gccacctgct gggggccatc gtgtaattta aacacggtgt tttgcacaat tatcttgtgc
     1561 cccaaatcca caaacgcgtt taaaaacgcc gcaataaaca gcggcaggct gcctggggtt
     1621 tggctgagtt taggcatggg gggtccttaa cactaaaatg ggtgacgagg cagggagtgt
     1681 aactgagctg ggcgggtttt gctttatttt gctgggtatt gggtaagaga ataactagtg
     1741 agtgtggttt cacttttgaa gaaaaaggcc tgtggtgaat ctaggcctac tcaaaagagc
     1801 gcgcgctgaa tctttgtgat caaacaagct gctaggaatg aaagtgaaga gggcgaaata
     1861 aagtaaacgg gcaaattggc gcgctagtga gtatttgcat ttagcataag ttcgtccttg
     1921 aacctagctg ggtggagatg gagttattgg cgaatgcgct tccagcctag cgattttaaa
     1981 tagagcgcaa catttgcagc gcagccgctg ctgggtattg tttgcgtgca aagcttaggt
     2041 gtgccactgc ccccgcgcag ggacatgcgc attacagagg ccattacctc gtcgtaactt
     2101 aatacgaact cattcgggca atcctcgcca tgggcgtaac tgcaaatacc aaatccaatc
     2161 cgcccaaccc tttatcacca tgacaattta agcaggcgcc att
//
LOCUS       DAOQGS010000665         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33112_length_2196_cov_3.162541, whole genome shotgun sequence.
ACCESSION   DAOQGS010000665 DAOQGS010000000
VERSION     DAOQGS010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33112_length_2196_cov_3.162541"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKZ52_17505"
     CDS             complement(<1..841)
                     /locus_tag="PKZ52_17505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNG61623.1"
                     /translation="MVQPPNNNNDATRLNTEQPAPNNDATRVTHLDATELSSHDATRV
                     TQPNDARTRLNTTGNSNLNTDNAATVVANTTSQKPTLSGTQSSTRTNTYGYGLGHRVA
                     PTPGARVLRDRFELIEPPLGSGGMGDVFLARDLVREEMQDSSPLIALKLLNETCRSLP
                     GALQALQREAKKAQTLSHPNIVTVFDFDRDGEDAFITMEYLKGESLKDYLRKQQKLSL
                     KEGHAILEKIARGLAYAHQEGFVHADIKPANIYITDTQMVKILDLGIAHAFTEAKKDA
                     PSAA"
     gene            complement(1009..>2196)
                     /locus_tag="PKZ52_17510"
     CDS             complement(1009..>2196)
                     /locus_tag="PKZ52_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61624.1"
                     /translation="LSSNLPMRMYGDVERFSQVVNILLNVIINQSEGCQILLEAEFTR
                     DQEQEGELHLTLSRQTLAAKKLFFQDLTDESIQLNLDLALAKGLADALGGNLDLAEGG
                     NAGEGVQLRFRLRMTMAEHEAQMVFQRPQFRGKRILLQHPNPRLLDIKRHELDAMGFV
                     VFTETQLKKAFNKLEESIGQGQALDAIVYYLEADDTDTIEFAEAILARSDLKFIKQLA
                     LISASSLNSPAIAALRNAANFHTIVRPAGIFELEIHLQPLISKIAQGDVAFITPEVLL
                     YNPDKKNLASKQWLNQFAVVTEVNTLDALFETIQAQPPVIIACVVDQLGMSEQMLLQL
                     RSFERDRQQEFFTPVIGIGSAEQAYSAYQMGVDHFLNKDDSGHTQVHELIYWRDVFIA
                     QQH"
BASE COUNT          478 a          499 c          631 g          588 t
ORIGIN      
        1 cggcggcgct gggggcgtct tttttcgctt cggtaaaggc gtgggcgata cctaagtcca
       61 atatttttac catctgggtg tcggtaatat aaatattggc cggttttata tcggcgtgaa
      121 caaatccctc ttgatgcgca taggcaaggc cacgggcaat tttttcaagg atggcgtggc
      181 cttcttttaa ggataatttt tgttgtttgc gcaaataatc ttttagggac tcacctttta
      241 aatactccat ggtgataaac gcgtcttcgc catctcggtc gaagtcaaat acggtcacaa
      301 tattcgggtg cgacagggtt tgcgcttttt tcgcttcacg ctgcagggcc tgcagggcac
      361 cgggtaagga gcggcaggtt tcgtttaaaa gttttagcgc aattaagggg gaggagtcct
      421 gcatttcctc ccgcactaag tcgcgggcta aaaatacgtc acccataccg ccggagccta
      481 agggcggttc aattaattca aagcgatccc gcagtacacg ggcgcctggg gtgggcgcca
      541 cccgatggcc caagccgtag ccgtaggtgt tggtgcgagt ggacgactgg gtgccggaga
      601 gggtgggttt ttgtgaagtg gtattggcca ccacagtagc ggcgttgtcg gtattgaggt
      661 tgctgttgcc agtggtattg agacgcgtgc gggcgtcgtt aggttgagtg acccgcgtgg
      721 cgtcatggct gctaagctcg gtggcgtcca gatgggtcac gcgggtggcg tcgttattag
      781 gcgcaggctg ctccgtgttt aaacgggtag catcattatt attgttgggc ggctgaacca
      841 tagcgaaatc tcggtggtgt aggacgtgag gataggctaa acgccgtatc tgtgccgtgc
      901 ggctttaaca ctccctgagc attttattaa ctcggcggtt tttatgccga gttaataaat
      961 gcgtaagcgc aggctgtgtg gctgagctat ttacacctgt gcgcggggtt aatgctgctg
     1021 cgctataaat acatcccgcc agtaaattaa ttcatgtact tgggtatggc cgctatcgtc
     1081 tttatttaaa aaatggtcca cgcccatttg gtaggcgcta taggcctgct cggcggagcc
     1141 tatgccaatg acaggggtga aaaattcctg ctggcggtcg cgctcaaagc tgcgcagctg
     1201 caataacatc tgctcggaca tgcccagctg atccactacg caggcgataa taaccggtgg
     1261 ctgggcctgg atggtttcaa acaatgcatc cagcgtattg acttcggtca ccacagcgaa
     1321 ttgatttaac cattgtttgc tggcgagatt ttttttgtcc gggttgtaca gcagtacttc
     1381 tggggtgata aacgccacat cgccctgggc gattttactg atgagcggct gcaggtgtat
     1441 ttccaattca aaaatacccg ctgggcgtac aatcgtgtgg aagttagcgg cattgcgcaa
     1501 tgcggcgatg gcggggctgt tgaggctgct ggcggagatt aatgccagtt gtttaataaa
     1561 tttaaggtcg gaacgggcca aaatggcttc ggcaaattca atggtgtccg tgtcatcggc
     1621 ctctaaataa tagactatgg cgtctagggc ctggccctgc ccgatggatt cctccagctt
     1681 attaaaggcc ttttttaatt gggtttcggt aaataccaca aaacccatgg catctaattc
     1741 gtggcgttta atatccagca ggcgcgggtt ggggtgctgc aataaaatgc gtttaccgcg
     1801 aaattgcggg cgctgaaaga ccatctgggc ttcgtgttcc gccatggtca tgcgcaggcg
     1861 aaaacgcagt tgtacacctt cacctgcatt gccgccttcg gctaaatcca aattgccgcc
     1921 gagggcgtct gctaagcctt tggcaagggc taggtctaaa tttaattgga tggattcgtc
     1981 ggttaagtct tggaaaaata attttttcgc ggcgagggtt tgccgcgaca gggttaaatg
     2041 caattcgccc tcctgctctt gatcgcgggt aaattctgct tccaataaaa tttggcagcc
     2101 ttcactttgg ttgataatga cattgagcag aatattcact acctgggaaa agcgctccac
     2161 atcgccgtac atgcgcatcg gcaggttcga ggagag
//
LOCUS       DAOQGS010000666         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33126_length_2195_cov_3.924299, whole genome shotgun sequence.
ACCESSION   DAOQGS010000666 DAOQGS010000000
VERSION     DAOQGS010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33126_length_2195_cov_3.924299"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKZ52_17515"
     CDS             complement(<1..758)
                     /locus_tag="PKZ52_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNG61625.1"
                     /translation="MKTHVLFKKHLSLAILASLVMSGCGGGYVDSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSTGGTVHATCPSTNAAAFQNFAVVVPGKIEAEDFDPAG
                     YMDSSETNEGGAYRTDTGVDIKSISGGNAVGWITNGEYLEYSVYVQNAGTYDIYVRSG
                     AVGEGRTLKITQCDQTLVQEFKVPNVADWGQFKTWKAGSVRLTAGIQKIRITAGATDY
                     LDLDWIHIGPYSGTIDPPDTVSTSSSSSSSSSSTS"
     gene            complement(1512..1949)
                     /locus_tag="PKZ52_17520"
     CDS             complement(1512..1949)
                     /locus_tag="PKZ52_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61626.1"
                     /translation="MLLDSKKADVFLEQYKLALAVANGNKAPTNRREFVQARESLYEL
                     PKTKQAALRKKISPAFLACINSALFGHYIYCRKYKNGYIFHSLDDGRFYRAVALTSPI
                     ETLFDNYWLTKTALVPFDGQIVCDGLFAGGGLVGKNRIGELRD"
BASE COUNT          572 a          544 c          505 g          574 t
ORIGIN      
        1 gacgttgagc tgctgctgga tgagcttgaa cttgaagtag aaactgtatc cggtggatcg
       61 atggtgccgg agtaaggacc gatgtgaatc caatccaaat ctaaataatc cgtagcgcct
      121 gcggtgatac gaattttttg gattccggcc gtcaagcgta cgctgcccgc tttccaagtt
      181 ttaaattggc cccagtcggc aacattaggc actttaaatt cctgcacgag ggtttgatcg
      241 cactgggtaa tttttaaagt tctgccttcg cccactgcac cggagcgtac ataaatatcg
      301 taggtgccag cattttgcac atacacggaa tactcaaggt attctccatt agtgatccaa
      361 cccaccgcat tgccgccgga aatagattta atatccacgc cggtatcagt gcggtacgcg
      421 ccgccttcgt tggtttcgga ggaatccata tagcctgctg ggtcaaaatc ttccgcctca
      481 attttgccgg gtactacaac ggcaaaattt tggaaggcag ccgcattcgt gctcgggcaa
      541 gttgcatgca cagtgccgcc ggttgatgag ctgctgctgg atgagctact cgaactcgaa
      601 gaagatgagc tggaagagga actggaactg gaggaactag atgagctgga cgagctgcta
      661 ctggacgaat ccacgtaacc accgccgcat ccactcatca caaggctggc taaaatagcc
      721 aagctcaaat gttttttaaa taatacgtga gtcttcatac aagcacctta taagtgattt
      781 atcacgctga actggctgtg gatttattat taaacgcggc agatgcggcc caagcctagg
      841 cccaccctct acctgtatcc gtaatagcca ccacttagcc tccatcgcca aattcaactg
      901 ctcatcactt taccaagtgc taaaggaaat tctgggtaat agctctcaat cccaaccatt
      961 ggttttattg taagactaaa atgttaattt tagttatgag catcacactc caaaagttgc
     1021 tctgccctca ctccagcggc ccaatactgt aataaaaaca tataaaacaa taatttaaat
     1081 ggccggaatg agggcctctc ggattgcata gagggcgaag ggcggtggtg aaatgtgagc
     1141 gatttcgaat ttattctgct gtgtttagca gttatggctg agcgggtatg gggaaattta
     1201 cacgccgtat attgccatgc agcaaaacca cggaagaaac aggctcggcc tgtttcccaa
     1261 tcggcggcga ttgagcggcg cacctaaatt cggctcgggc atttgggtta cttttaaccc
     1321 ggcggcaaaa tagatcgtcc tgatctattt tgccttcgct ccgctgacac tagcaaaaat
     1381 gcgtggattt tctggtttta gcggagctcg cggcggcttg cgcctttggt gcggcatcct
     1441 tgccgcactt attcacccgg cataataagc cgcccggtta ctatattgcc gagccgtttt
     1501 tcgctcctgt atcagtcgcg caattcccct atcctatttt tgccaactaa cccgccgcct
     1561 gcgaataaac catcacacac tatttgccca tcaaaaggca ccaacgcagt tttagtcagc
     1621 cagtagttgt cgaagagggt ttcaattggg ctcgtcaagg cgacagctct gtagaagcgt
     1681 ccgtcatcta aactgtgaaa aatataacca tttttatatt ttcggcagta aatatagtgg
     1741 ccaaataaag cgctgtttat acacgccaaa aacgcggggc tgattttttt gcgcagtgct
     1801 gcctgcttag tttttggcag ttcatacagc gattctcgtg cctgcacaaa ttcacgccga
     1861 tttgtaggag ccttattgcc gttggcaaca gcaagtgcca atttatattg ctcaagaaag
     1921 acatccgctt ttttactatc caacagcatt agttagcact caataagacc cattaaatat
     1981 cgccaatttt acagccctgt cacgataggc caagaacact aaaattccga gctacattat
     2041 gtcgagcaga tatttatagc gccgattaaa taatgtccac ccgcgcaggg aaaatgccgg
     2101 ttactattaa cccggcggcc aaaaacaggc gaaaatacgc ggattttctt gtttttgcgc
     2161 agctcgcggc ggtttgcacc gccgagtgcg gcatc
//
LOCUS       DAOQGS010000667         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33220_length_2191_cov_5.154963, whole genome shotgun sequence.
ACCESSION   DAOQGS010000667 DAOQGS010000000
VERSION     DAOQGS010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33220_length_2191_cov_5.154963"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PKZ52_17525"
     CDS             complement(<1..1021)
                     /locus_tag="PKZ52_17525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNG61627.1"
                     /translation="MLDIERAVAGRFPNFYNKPLLRKPALRLLKALLRQDAVNEFMRK
                     HQALDGGEFIDAIFDYFDFSYSVSQRDRANIPAEGRVVIVANHPIGSLDGLALIKLVT
                     EIRPDVKILANQMLSFFTPLKRFLISVDNFAAVPQARKGIKAILQALSEEQAVIIFPA
                     GEVSRAQPTGVKDGQWKPGFLHVARRANAPVLPIFIKAKNSWYFYGASWLFKPLSTLL
                     LAQEMFNKQSKTIHFHVGEAINPEQLQSDQLTDRTLVKRLRKHLYKIGKGKASQFITQ
                     KTIAHPEPRELLIKELAEAERLGATSDGHEIYLADYHPGSALMRELGRQREIAFRKVG
                     EGTGAK"
     gene            complement(1121..1513)
                     /locus_tag="PKZ52_17530"
     CDS             complement(1121..1513)
                     /locus_tag="PKZ52_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG61628.1"
                     /translation="MLKRIHHAAIICSNYAVSKRFYSECLGLVIIAEHYRAERDSYKL
                     DLALPDGGQIELFSFPAPPARPSFPEACGLRHLAFAVEDLDACVRSLMAKGIALQAIR
                     VDEYTGQRFVFFTDPDGLPLELYECTGG"
     gene            1921..>2191
                     /locus_tag="PKZ52_17535"
     CDS             1921..>2191
                     /locus_tag="PKZ52_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61629.1"
                     /translation="MFASFTSIRFILALLTVLAIALKPLIPQRTLQLYPAAQNTLSIH
                     GPPEPNGQPSVRWLDEANGSWRCAITQSNQIKSNCIMVIDWLPNHA"
BASE COUNT          599 a          588 c          488 g          516 t
ORIGIN      
        1 gtttagcgcc cgtgccctcg cctacttttc gaaaggcaat ttcccgctgg cgccccaact
       61 cgcgcattag ggcagagccg gggtggtaat ccgccagata tatttcatgg ccatcgctgg
      121 tggcacccag ccgctccgcc tccgctagct ctttaattaa taattcccgc ggctccgggt
      181 gggcgatggt tttttgggta ataaattggg aggctttgcc cttgccgatt ttgtacaaat
      241 gtttacgcag gcgtttaacc agggtgcgat cggttaattg atcggactgc agctgctctg
      301 ggttaatggc ttcgcccaca tgaaaatgaa tggttttgga ttgtttatta aacatctcct
      361 gagccaataa tagggtgctc aagggtttaa ataaccaact ggcgccataa aaataccagg
      421 agttttttgc cttaataaat atgggcaata ccggcgcatt ggcgcgcctg gccacatgta
      481 aaaaacccgg cttccattgg ccgtctttta caccggtggg ctgggcgcgg gagacctcac
      541 cggcgggaaa aataatcacc gcctgctcct cacttaaggc ctgcaaaata gccttaatac
      601 ccttgcgcgc ctgaggcacg gcggcaaaat tatccactga aattaaaaag cgtttcaaag
      661 gtgtgaaaaa actcagcatt tgattggcaa ggatttttac atccgggcga atttctgtga
      721 ccaatttaat gagggctagg ccgtctaatg accctatcgg atggttggcg acaattacca
      781 ctcggccttc ggcggggata ttggcgcggt cgcgctggct gacgctgtag gagaaatcaa
      841 aataatcgaa aatagcatca ataaattcac cgccgtccaa ggcctgatgc ttgcgcataa
      901 attcattcac agcatcctgc cgcaatagcg cttttaataa ccgcagcgca ggcttgcgca
      961 acagcggttt attgtaaaaa ttaggaaacc gccccgctac tgcccgctca atatccaaca
     1021 tagccaacac ctaacaacct aatttggctc agcctaggtg gattccattg caaaattatg
     1081 acgggcgtgg cggatacata acggggcaaa aatcggcggg ttaaccccct gtgcactcat
     1141 acaattcaag gggcaaacca tctggatcag taaaaaatac aaaccgctgc ccggtatatt
     1201 catctacacg aatcgcctgt agggcaattc cctttgccat aaggctcctg acacaggcat
     1261 ctaaatcctc cacggcaaaa gccaaatggc gcaaaccaca ggcctctgga aacgaaggcc
     1321 gcgcaggtgg tgcgggaaac gaaaacaatt caatctggcc gccatcgggc agggccaaat
     1381 ccaatttata ggaatcccgc tcggcacgat aatgctccgc tattattacc agccccaaac
     1441 actcgctata aaaccgtttg gacaccgcgt aattggaaca aataatagcc gcgtggtgaa
     1501 ttcgctttaa catgtcacaa tatccgctaa aaggctgccc ttaataattg ccattgtatt
     1561 aacccggcgg caattagtgc cgggttaata aatcgctaaa caacgccatt aataattacc
     1621 acaacaaaac tcaacaaaag tattttcctg cgcccccaaa cccaaccgac ctggctcaag
     1681 ccgagcggct aggttattca cataactgca tacaaggcta tcccttattc acattttatt
     1741 gttcgcgcgt taactgccga catcggcaaa aacagcccta aatgccgtga aaaaaccgcc
     1801 gggctccatc caaatccgcc tgaccatcca tgatctatac tcaatcctag cttgcctacc
     1861 cgatccatag tatgtgttag ggatggcgaa ttcatcgact aaacatataa attggacttt
     1921 atgttcgcat cctttaccag tatcaggttt atcctggcgc tcctgacggt attggcgatt
     1981 gcgttaaaac cacttattcc ccaacgcaca ttacagctgt atccagccgc ccaaaatacc
     2041 ctctccattc acgggccacc ggagcccaac ggccaaccaa gcgttcgctg gcttgacgaa
     2101 gccaatggca gttggcgctg cgcgatcaca caaagcaacc aaattaaaag taattgcatt
     2161 atggtgatcg attggttgcc gaatcatgca g
//
LOCUS       DAOQGS010000668         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33295_length_2188_cov_5.412096, whole genome shotgun sequence.
ACCESSION   DAOQGS010000668 DAOQGS010000000
VERSION     DAOQGS010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33295_length_2188_cov_5.412096"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKZ52_17540"
     CDS             <1..745
                     /locus_tag="PKZ52_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNG61630.1"
                     /translation="AMLSVVPPPQMVPTMEAGTTAGYCVGEPWNQQAVTKGLGVPVVT
                     SENLWPNGSDKVFGVTKAFADKNPNTTIRTVKALIRASAWLDANNNANRPEAVKIISR
                     PAYVGADEKVIANSMTGTFEFEKGDKRPIPDFNKFFRGYYGMPYYSDAIWWTTQMRRW
                     GHIPEQKPDAWYMEIAKKALRPDIYVTAAKELIAEGKMKATDFPDLDKETFMKAPVKS
                     PLDGIEFDGNKPNEYLEKFTIGLKGNQKA"
     gene            849..1823
                     /locus_tag="PKZ52_17545"
     CDS             849..1823
                     /locus_tag="PKZ52_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61631.1"
                     /translation="MSANLISAPNSFFVSALLKQCVKSIALPLVGFAIFLLLWEFGSS
                     KVQTSLGQFPGPAIVFNQVQGLYQEYKEDKASRAAFYERQEKRFAEARAQDPNYVAEH
                     RQYSGKPTFIQQIFTSLKTVFTGFLLASLIAIPLGIAVGLSANLYAAINPLIQLFKPI
                     SPLAWLPLVTIVVSAVYSNPDGVPKSFITSSMTVMLCSLWPTLINTAVGVTSVSSDLI
                     NVSRVLRLNWLRHVRSIVLPSAVPMIFTGLRLSLGIAWMVLIAAEMLAQNPGLGKFVW
                     DEFQNGSSDSLGRIMVAVLTIGVIGYLLDRGMLLLQKVFSWDKQAILR"
     gene            1850..>2188
                     /locus_tag="PKZ52_17550"
     CDS             1850..>2188
                     /locus_tag="PKZ52_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG61632.1"
                     /translation="MKPILELSQVSIEFKTPKGVFKALDNINLKIGQGEFVSLIGHSG
                     CGKSTVLNIVAGLLKATAGGAVLEGKEVNEPGPERAVVFQNHSLLPWLTAYENVELAV
                     KQIFKGKKSKA"
BASE COUNT          564 a          566 c          498 g          560 t
ORIGIN      
        1 ggcaatgtta tctgttgtgc caccgccaca aatggtgccc accatggaag caggcaccac
       61 cgcgggttac tgtgtgggtg aaccctggaa ccaacaggcg gtaactaaag gtttaggcgt
      121 accggtcgtc actagcgaaa acctatggcc caacggctcg gataaagtgt ttggcgtaac
      181 caaagccttt gccgacaaaa accccaacac caccattcgt accgtaaaag cccttattcg
      241 cgcctctgcc tggctcgacg ccaacaacaa tgccaaccgc ccagaagcgg tgaaaattat
      301 ttcccgccct gcgtatgtag gtgccgatga aaaagtgatt gccaacagca tgaccggcac
      361 ctttgaattt gaaaaaggcg ataaacgccc aatccccgat tttaataaat ttttccgcgg
      421 ctattacggc atgccctact actcggatgc catctggtgg actactcaaa tgcgtcgttg
      481 gggccatatt cctgagcaaa aacccgatgc ctggtacatg gaaatagcca aaaaagcctt
      541 gcgtcccgat atttatgtaa ccgctgcaaa agaattaatt gctgaaggca aaatgaaggc
      601 tactgacttc cccgatcttg ataaagaaac cttcatgaaa gctccagtta agagtccttt
      661 agacggtatc gaattcgatg gcaacaagcc gaacgaatat cttgaaaaat tcaccattgg
      721 cttaaaaggc aatcaaaaag cctaaccacc tgcatcactc gcttttctcc ctgctcgcgg
      781 tacacggatc gaccgctttt tatcttcggc ctggccgagt tgttttgccg cattatgagg
      841 gtcagcttat gtctgccaat ctgatttccg cccctaattc tttttttgtc agcgcattat
      901 taaaacaatg cgtcaaatcc atcgcgctgc ccctggttgg gtttgccatt tttctactct
      961 tgtgggaatt tggctccagc aaagttcaaa catccctggg gcaatttccc ggccccgcca
     1021 ttgtatttaa tcaagtgcaa gggctttatc aggaatacaa agaagataaa gcctcaaggg
     1081 cggcgtttta cgagcggcag gaaaaacgct ttgccgaagc ccgcgcccaa gatccaaatt
     1141 atgtggccga gcaccgccaa tacagcggca aacccacctt tattcagcag atatttacca
     1201 gcttaaaaac tgtgtttacc ggctttttgc tggcctcgtt aatcgccatt ccattgggca
     1261 ttgccgtggg cttaagtgcg aatttatacg cggctattaa cccgctgatt caattattca
     1321 aacccatttc accgctggcc tggctgccgc tggtcaccat tgtggtgagc gccgtgtaca
     1381 gcaacccaga cggagtgcct aaatcattta ttacctcatc tatgaccgta atgctgtgca
     1441 gcttgtggcc cactttaatt aataccgctg taggcgttac atcggtgagc agcgatttaa
     1501 tcaacgtcag ccgcgtattg cgcttaaatt ggttgcgcca tgtgcgctcc attgtattgc
     1561 cctcagctgt acccatgatt tttaccggcc tgcgtttatc cctcggcatc gcctggatgg
     1621 tgttaattgc cgctgaaatg ctggcgcaaa accccggttt aggtaaattc gtgtgggatg
     1681 aatttcaaaa tggcagctcc gactccttag gccgcattat ggttgccgta ctcaccatcg
     1741 gcgtaatcgg ttatttgtta gatcgcggca tgttgctgct gcaaaaagta ttttcctggg
     1801 ataaacaagc gattctgcgc tagcccccaa ttaagatgtg gagtgtttta tgaaacctat
     1861 tttagagtta tcccaagtca gcatcgaatt taaaaccccc aagggtgtat ttaaagcgct
     1921 ggacaacatt aatttaaaaa tcggccaagg cgaatttgtc tccttaattg gccactccgg
     1981 ctgcggcaaa tccactgtac tgaatattgt tgccgggctc ttaaaagcca ccgctggcgg
     2041 cgctgtgcta gaaggtaaag aagtcaacga gccaggccct gagcgggcgg tggtatttca
     2101 aaaccactcc ctgctgccct ggctgaccgc ctatgaaaat gtggagctgg cggtaaaaca
     2161 aatatttaaa ggcaaaaaat ccaaggct
//
LOCUS       DAOQGS010000669         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33331_length_2187_cov_3.642589, whole genome shotgun sequence.
ACCESSION   DAOQGS010000669 DAOQGS010000000
VERSION     DAOQGS010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33331_length_2187_cov_3.642589"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2178)
                     /gene="mrcB"
                     /locus_tag="PKZ52_17555"
     CDS             complement(<1..2178)
                     /gene="mrcB"
                     /locus_tag="PKZ52_17555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HNG61633.1"
                     /translation="MYARPLELYEGMSLSSQMLDDQLAVLGYRANDGVQDAGQFNKQS
                     KGKKGAESSYTIYSRGFEFADKTDAPQAFKLDIVGNKVTALTDPTGNPLPLVRLEPEE
                     IGGIYPSQMEDRLLVKIDQIPKLLGEGLIATEDKAFMEHHGVSPMAIVRAMWVNLSSG
                     QVVQGGSTLTQQLVKNFYLSNERSLKRKAREAIMALLLEKHYSKSAIFETYINEVYLG
                     QSGAREIHGFALAAKHYFRQPLNELNAGQIAYLIGLCKGASYYNPWNHPQRALERRNV
                     ILNVMAKEGLLSPAELQAQLDKPLGVLPPTDNSLQTFPAFVELVKRQLQQEYKIEDLR
                     SEGLKIFTTLSSVLQRQAEASLVEKTHSLEKKYKQDLQAAMVVTSVGTAEVLALIGDQ
                     APHTEGFNRALDSRRSIGSLMKPFVYLTALERPEEYNLGTILSDTPVTYTLDNGQEWS
                     PKNSHGESHGDVPFYAALAHSYNQATVQLGMQLGLPAVIKTLRAAGYHDDIAAVPSLL
                     LGSIDAPPIQMAELYHTLAADGVHAPLRVVQEVIDQEGHPLKRYQLEIQQQFSQEAIF
                     ALHHAMYMVFREGTARSVAGLFGNTRLAGKTGTTNDQRDSWFAGFSSDLMAVVWMGRD
                     DNQPTPVSGAGGALQVWADFMAKIPTKGLDEEPPSGMDYYYIDGATGLLSERGCPGAL
                     YLPLRLEQVPSEMSSCGSSLLDRLLPSSAPAPNTPAAPANPNAP"
BASE COUNT          370 a          620 c          702 g          495 t
ORIGIN      
        1 gggggcattg gggttggcgg gggcggctgg ggtgttgggg gccggtgcgc tggagggcag
       61 taggcgatcg agcaggctgc ttccacagct gctcatttca ctgggcacct gttccagccg
      121 caggggtaaa tacagggcgc cggggcagcc gcgctcgctg agcaggccgg tggcgccgtc
      181 gatgtagtaa taatccatgc cgctgggggg ctcttcatcc aggcccttgg tgggaatttt
      241 cgccataaaa tcagcccaca cttgcagcgc tccgccggcg ccggatacgg gggtgggttg
      301 gttgtcatcg cggcccatcc acaccaccgc cattaagtcg ctgctaaagc ctgcaaacca
      361 gctgtcccgc tggtcgttag tggtgccggt tttgcccgcc aggcgggtgt tgccaaataa
      421 tcctgccacg ctgcgggcgg tgccttcgcg gaacaccatg tacatggcgt ggtgcagggc
      481 gaagatggcc tcctggctaa attgctgttg gatttccagt tggtagcgtt tgagggggtg
      541 gccctcctgg tcgatcacct cttgcaccac ccgcaggggc gcgtgtacgc cgtcggcggc
      601 cagggtgtgg tacagctccg ccatttgaat gggcggcgca tcgatactgc cgagcaggag
      661 ggagggcacg gcggctatgt cgtcgtggta acccgctgct ctaagggtct tgatcacggc
      721 gggcaggccc agctgcatgc ccagctgcac cgtggcctgg ttgtaggagt gggcgagggc
      781 ggcataaaac ggcacatccc catggctctc gccgtgggag tttttcggcg accactcctg
      841 gccgttatcc agggtgtagg tgaccggggt atcgctcaaa atagtgccca ggttgtactc
      901 ctccgggcgc tccaaggcgg tgaggtatac gaagggtttc atcaaggagc cgatggagcg
      961 gcgggaatcc agggcgcggt taaagccctc ggtgtggggg gcttggtcgc caatcagggc
     1021 cagcacttcg gcggtgccca cgctggttac caccatggcg gcctgcaaat cctgcttgta
     1081 tttcttttcc agactgtggg ttttctctac taggctcgct tcggcctggc gctgcaacac
     1141 gctggacagg gtggtgaaaa tttttaaccc ctcgctgcgc aggtcctcaa tcttatattc
     1201 ctgctgcagc tggcgcttca ctagctccac gaaggcgggg aaagtttgca ggctattgtc
     1261 cgtcggcggc agcacgccca ggggtttatc cagctgcgct tggagttctg cggggctgag
     1321 caggccctct ttggccatta catttaaaat cacgttgcgc cgctcgagtg cacgctgggg
     1381 gtggttccag gggttgtagt aggaggcgcc tttgcacagg ccgatcaaat aggcgatttg
     1441 gcctgcgttc aactcgttta agggttggcg aaaatagtgt ttggcggcca gggcaaagcc
     1501 gtggatttcc cgtgcgccgc tctggcctag atacacctca ttaatataag tctcaaaaat
     1561 cgcgcttttg ctgtaatgct tttccagcag cagggccatg atggcctccc gcgccttgcg
     1621 tttgagggag cgctcgttgc tcaggtaaaa gtttttcacc agctgctggg tgagggtgct
     1681 acccccttgc accacctggc cgctgctgag gttcacccac atggcgcgca ctatggccat
     1741 gggcgagacg ccgtggtgct ccataaaggc tttatcctcc gtggcgatca agccttcgcc
     1801 gagcaacttg gggatttggt caatcttcac cagcaggcgg tcttccattt ggctggggta
     1861 aatgccgccg atctcttccg gctccaagcg caccaggggc agcgggttgc cggtgggatc
     1921 ggtgagggcg gtgaccttat tgccgacaat atccagctta aacgcctggg gtgcatcggt
     1981 tttatcggcg aactcaaacc ctcggctata gatggtgtag ctgctttcag cgcctttttt
     2041 acccttgctc tgtttgttga attgcccggc atcctgcacc ccatcgttgg cgcggtagcc
     2101 cagcaccgcc aactggtcat ccagcatctg gctggacagg ctcataccct catacagttc
     2161 taagggccgc gcatacaccc gcgccgg
//
LOCUS       DAOQGS010000670         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33435_length_2183_cov_4.848214, whole genome shotgun sequence.
ACCESSION   DAOQGS010000670 DAOQGS010000000
VERSION     DAOQGS010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33435_length_2183_cov_4.848214"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..489)
                     /gene="fdxB"
                     /locus_tag="PKZ52_17560"
     CDS             complement(181..489)
                     /gene="fdxB"
                     /locus_tag="PKZ52_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015816838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HNG61634.1"
                     /translation="MAESAENVIIGRTRGGEQWIPEYVTELNAKTCIGCGRCYKVCPR
                     DVFELVERASVVEDDDDFYDEDDEMMVMTIKNPLDCIGCVSCSRVCPKNCHTHQALPG
                     "
     gene            complement(512..721)
                     /locus_tag="PKZ52_17565"
     CDS             complement(512..721)
                     /locus_tag="PKZ52_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HNG61635.1"
                     /translation="MNEEELKALKKDVSTKKRIATDWASQIHDLVEDRLFTDYGNLVE
                     LARQTATACEQWAEAKNTLEAVEAT"
     gene            complement(1620..2087)
                     /locus_tag="PKZ52_17570"
     CDS             complement(1620..2087)
                     /locus_tag="PKZ52_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HNG61636.1"
                     /translation="MTTDVIDEKAPLLAHPFVKQVVTQLRAMDSYGTYDTWTDCRVLD
                     PLILTKERRREIPVVGDPDDTTISRVKAFYNAISCRVEAESGLMAVPMINLSHEGFGR
                     AIITVGKLIVMDKILRDVHRFGFESLEKLIDEVDKLTNKAVETINAHREVAQL"
BASE COUNT          469 a          585 c          538 g          591 t
ORIGIN      
        1 gcagtccctc gacgttctcc accttgcggt agatcctttc tccggcgacg gtgcggcact
       61 tctcctcgaa caaggccttg ccatccgtca tgcgcgcctg tgtcgcttcc cgctcacccg
      121 tctgcggtgc ctgggcgcag gaagcggtgg cgcaaagggc gagcccgcct aaagcggggc
      181 ttagcccggc agcgcctggt gggtgtggca gtttttcgga cacacgcggg agcaggacac
      241 acaaccgatg caatcgaggg gatttttaat ggtcatcacc atcatttcat cgtcctcgtc
      301 gtaaaagtcg tcatcgtctt caacaacgct tgcccgctcc actaattcaa atacatcccg
      361 agggcacacc ttgtagcagc gcccgcaacc gatgcaggtt ttggcgttta actccgtaac
      421 gtattccgga atccactgct cgccccctcg ggtgcggcct atgatgacgt tttcagctga
      481 ttcagccatg gtgtacctcc gcgctagtaa ctcaggtcgc ctcgacggct tccagggtgt
      541 ttttggcctc agcccattgt tcgcaggctg tggcggtttg ccgcgccagt tccactaaat
      601 tgccgtagtc ggtgaagagc ctatcttcca cgagatcgtg aatttgtgag gcccaatcgg
      661 tggcgatgcg ctttttagtg ctgacgtctt tttttaaggc tttgagttct tcttcgttca
      721 taaggggtgg tctttttgct gggtggaaat tttgttttgc tttttcgtaa tcgggttttt
      781 cgaattttcg ctctggcgct tctcgttccc acgctccgcg tgggaatgca tacaagctga
      841 tgtttccttt tctcgttccc gcgctctgcg tgggaatgca taactgtcgt ttcttttaaa
      901 actcaattct cagtgtaagt accgcgcctc cggcgggtca cttttctttg cgtggccaaa
      961 gaaaagtaac caaaagaaaa gccaccctgc ttttgggttt tgctgcgcaa aactaccttc
     1021 gctccggcac aatttcgggg gtcgtcgcga agggccatcc atggccctcc actcctcaat
     1081 tgggcatccc gcccaattga cccccaaaat tgcacctcca ctcagccaaa agaagggttc
     1141 taccttggcg cctgacaccg taactttggt agatattggt agatagatta aaaagtaaat
     1201 tctcgttccc actctccgcg tgggaatgca tacctggccc tgactcagat ctacccttct
     1261 actttcaacc aatgcgcgga cacatcgaat atcacttcat tctgagacaa ctaacacttt
     1321 ctaaatctgt ccttctaaag aataaattct cgggttaatc acaacatctt aaacgagcct
     1381 cttctctttg attcattctc acgctttgcg tgagaatgag gatcaccgct taaaaaattt
     1441 ctcgtttacc cgccactaca aaggcgattt cctgttttca ttgccagaga atacctacaa
     1501 gtaaaaaacc cgcgttcaat caacagattt tattaacact caaaaaaata aaatatcgcc
     1561 ctgattttcg cacccacaaa acaagtgcga tttaatcaaa cgataaatcc aaccgagcct
     1621 taaagctgcg ccacttcccg gtgggcgttg atggtttcca cggctttgtt ggtgagtttg
     1681 tctacttcgt cgatgagttt ttctaaagac tcaaaaccaa agcggtgtac atcccgcagg
     1741 attttgtcca tcacaattaa tttgcccacg gtgatgatgg cgcggccgaa gccttcgtgg
     1801 gagaggttaa tcatgggcac ggccatgagg cctgattcgg cctctacgcg gcaggagatg
     1861 gcgttgtaaa aggcttttac ccgggagatg gtggtgtcgt ctgggtctcc tactacgggg
     1921 atttcccggc ggcgctcttt ggtgaggatt aaggggtcga ggacgcggca gtcggtccag
     1981 gtgtcgtagg tgccgtagct gtccatggcc cgcagttggg tgaccacttg ttttacaaag
     2041 gggtgagcga gcaagggggc tttttcgtcg attacgtcgg tagtcataac aagtgtccaa
     2101 tgatggtgta gttaaaagtg gttattgggt tgtgcaggct caatcgcccc agccttccat
     2161 ctccatagcg tcaaagcgac cac
//
LOCUS       DAOQGS010000671         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33460_length_2182_cov_4.054067, whole genome shotgun sequence.
ACCESSION   DAOQGS010000671 DAOQGS010000000
VERSION     DAOQGS010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33460_length_2182_cov_4.054067"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1511)
                     /gene="ribD"
                     /locus_tag="PKZ52_17575"
     CDS             complement(435..1511)
                     /gene="ribD"
                     /locus_tag="PKZ52_17575"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNG61637.1"
                     /translation="MANHEHWMGQALKLAEQGINTTTPNPAVGCVLIGADGSLVGQGY
                     HQQAGGPHAEVFALRDAGDKARGATAYVTLEPCNHQGRTGPCSQALIDAGIKTLVYAM
                     QDPNPLVCGQGLARLRAAGVEVIGPYLEAEARELNRGFIKRMTQGRPFLTCKLAMSLD
                     GRTAMANGQSKWITGPAAREDVQTLRARSCAIITGAGTVLADNPSLTWRCNRPAGVSR
                     QPVRIVLDRHLRTPVSAAILQEPGTTWLISPQTVAANSPLAPFVKNLPDELPALMDWL
                     GAEGCNQVLLEAGAKLAGRFLAARLIDELVVYLAPTLMGSRALPLFDLQLDHMSERIG
                     LSIQSVSAVGVDLKITAVPKGPHY"
     gene            complement(1511..1963)
                     /gene="nrdR"
                     /locus_tag="PKZ52_17580"
     CDS             complement(1511..1963)
                     /gene="nrdR"
                     /locus_tag="PKZ52_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNG61638.1"
                     /translation="MHCPFCNQIDTKVIDSRLMADGSQVRRRRECIACKERFTTFEVA
                     ELVMPRIIKQDGTRQPFDEQKLRNGLLRALEKRPVSVERIEAAVNSVKHYLQALGERE
                     VKSLVVGERVMEELRALDGVAYVRFASVYRSFQDISEFRQELDRLEKD"
     gene            complement(1973..>2182)
                     /locus_tag="PKZ52_17585"
     CDS             complement(1973..>2182)
                     /locus_tag="PKZ52_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNG61639.1"
                     /translation="RDTIGFATVRGGDVVGDHTVMFMAEGERVEITHKASSRMSFARG
                     AVRAAVWLNGQPNGLFDMQDVLNLK"
BASE COUNT          463 a          644 c          619 g          456 t
ORIGIN      
        1 ggaaggtcat tttgccatct ggcgcggggg ttttacacaa ttctacgaag agcctaaaat
       61 atatttcgat ccgattgctg gcttcgatat ttattctgca agaatgcggt tctaatcatt
      121 gttagccgaa cgctccgcaa caaacagcgc acggtagctc agccttgagg cagaagaatt
      181 atcaacttca accgcagccg gccaggaaga accaatgctg atcccggcaa ggcgccagag
      241 tctgcgtcgc tcgcattagg aattctgtaa tcaattgtaa agacatgatg aacgacaaca
      301 ttaggctact tgttagagca ccacagccca ttggataagg ctgcgtttgt actctcgcca
      361 gcagatttcg aagagccccg atgcgcctaa cggagcttac cgggaggagt caggcgcgct
      421 atgaatccgc aagtctaata atgtgggcct ttaggcactg cggttatctt caaatccaca
      481 cccactgcgc taacgctttg aatggacaac ccaatacgct cactcatgtg gtctaactgc
      541 aggtcaaaca gaggtagggc acggctaccc ataagtgtcg gcgccaggta aaccactaat
      601 tcatcaatta accgcgctgc taaaaaccgc ccggcaagtt tcgccccggc ttccaacaac
      661 acttggttgc agccctcagc ccccagccag tccatgagcg caggtaactc atcgggcagg
      721 ttttttacaa aaggcgccaa aggagaattg gccgcgacgg tttgggggct gatcaaccag
      781 gtggtgcccg gctcctgcaa aatggccgcg ctcacagggg tgcgcaagtg gcgatcaagc
      841 acgatgcgca cgggctggcg gctgacgccc gcggggcggt tgcagcgcca ggtgaggctg
      901 gggttatccg ccagcaccgt gcctgcgccg gtgataatcg cgcagctgcg ggcgcgcagg
      961 gtctgcacat cttcgcgggc ggcagggcct gtgatccatt tgctctggcc attggccatg
     1021 gccgtgcggc catccaaact catggcgagt ttacaagtga gaaacgggcg gccctgggtc
     1081 atgcgtttga taaagccacg attgagttcg cgggcctcgg cctccaaata ggggccgatc
     1141 acctctacac cggctgcgcg caggcgcgca agaccctggc cgcagaccag tggattcggg
     1201 tcctgcatgg cgtagacgag ggttttgata ccggcgtcga tcagtgcctg gctgcagggg
     1261 ccggtgcggc cctggtggtt gcagggctct aaagtgacat aggcggtggc gccacgggct
     1321 ttgtccccgg cgtcgcgcag ggcgaacact tctgcatggg ggccaccggc ttgttggtga
     1381 taaccctgcc ctaccaggct gccatcggcg ccgataagca cacagcccac cgccgggttg
     1441 ggcgtggtgg tgttaatgcc ctgctcggca agctttaggg cctggcccat ccagtgttcg
     1501 tggtttgcca ttaatctttc tcaagccggt ccagctcttg gcggaactcg ctgatgtctt
     1561 gaaagctgcg gtacacagag gcaaaacgta cataggcaac gccatcgagc gcccgcagct
     1621 cctccatcac acgctccccc accaccaggg acttcacttc ccgctcaccg agggcctgta
     1681 aataatgctt cacactgttt accgctgctt cgatgcgctc cacgctcacc gggcgttttt
     1741 ccagcgcgcg caataaacca ttgcgcagct tttgttcatc gaaaggttgg cgcgtgccgt
     1801 cttgtttgat gatgcgcggc atcaccaact ccgccacttc aaaggtggta aagcgctctt
     1861 tgcaggcgat gcactcccgg cgacggcgca cctggctgcc gtcagccatc aggcgcgaat
     1921 cgatgacctt ggtatcaatt tggttacaaa aggggcaatg catataggtg ccttatttca
     1981 ggttgagcac atcttgcata tcaaataatc cattgggctg gccgtttaac caaaccgccg
     2041 cgcgcaccgc gccgcgggca aaggacatgc ggctggaggc cttatgggtg atttccaccc
     2101 gctccccctc ggccataaac atcaccgtgt gatcccctac tacatcgccg ccgcgcacgg
     2161 tggcaaaacc tatggtgtcg cg
//
LOCUS       DAOQGS010000672         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33653_length_2175_cov_4.366509, whole genome shotgun sequence.
ACCESSION   DAOQGS010000672 DAOQGS010000000
VERSION     DAOQGS010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33653_length_2175_cov_4.366509"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..632)
                     /locus_tag="PKZ52_17590"
     CDS             complement(66..632)
                     /locus_tag="PKZ52_17590"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HNG61640.1"
                     /translation="MALKAVSVLTPLVGQLVLAATLLSAQPASAQMVDLGDFTAPSTL
                     SYQNSFNGQHNQFTDWYAFSVPATQFNGITASISFAQLFGVQLTSELYHGSLSGNQVN
                     LGAYITGGATGQTVFGNAVQTTSVIQPTWLTGGDYLIKVSGAVIGSLGGSYAGIANMA
                     PVPLPPSLPLVGLGLGLLALLKSVRKRA"
     gene            1270..2085
                     /locus_tag="PKZ52_17595"
     CDS             1270..2085
                     /locus_tag="PKZ52_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61641.1"
                     /translation="MTKKTLCAFIGLAGFILAAHSAHAAPVSLKVEIQQTNDDGSLVN
                     GLRTSKTWDLSEETLWQPTPENPEVYDLITTDLKWKNKSTLAFGSAAEVYVNDLKYDA
                     DPLLSFDITLANNTAFNQTYSLSYNTPLLPMLTGVVNASSNLTAVLTDVNHLGGAKIS
                     PLAGSSILRAFDITVNGTEIPKNVDIGNSFSIANGSASKNWSAVNTLVCGTGDFACET
                     MSAVLTLTLSKGDKVRLYGEVEQIQAVPVPNSAVLLGSMLALLAGVRRKLRAA"
BASE COUNT          551 a          454 c          541 g          629 t
ORIGIN      
        1 attcgcctag ctagtgcttc gccgcctaca tttttcagag gttccctagg tatttcagta
       61 agcttttaag cgcgtttgcg cactgatttt agcagcgcta ataagcccaa acccaggccg
      121 actagcggca ggctcggggg taaaggcact ggtgccatat tggcaatacc tgcatagcta
      181 ccgcctaggc tgccgattac ggcgccactg acctttatta aataatcacc gccggtaagc
      241 catgttggtt gaattaccga ggtggtttgt acagcgttgc cgaaaactgt ttggccagtg
      301 gctccgccgg tgatgtaagc gcctaggttt acttgattgc cacttaaact gccgtggtac
      361 agctcgctag tgagttgcac gccaaataat tgcgcaaagg aaatagacgc ggtaatacca
      421 ttaaattgtg ttgcaggcac tgaaaaagcg taccaatcgg taaattggtt gtgctgcccg
      481 ttgaagctgt tttggtagga gagtgtggag ggtgcagtaa aatcgccgag atcaaccatt
      541 tgtgcgctcg ccggctgagc ggacagcaat gtggccgcca gcactagctg gcctacaagt
      601 ggggttaata cagaaactgc tttgagtgcc atgacgggta cctgtgtaag ctgtgtagta
      661 ggtatgaacg gggcgattga gtgcgaaaaa attattaagg aagcaaataa agccactgta
      721 ataatggtga tatttgcatc gatattttgg acacgccggt aatataaccg acacatattt
      781 agcagtgcaa tatcgcaaca catagttaat ccgcgccagc ctcctgcagg atgggcgggg
      841 gggaagcttg cggttctatc tgattgtttt ttatgggttt atcctggttg tttgggcgcc
      901 gggtggaggc tcgcgatgag aataattttt tgcgacaagt gtgtattttg tgtttctttt
      961 ttgttacaaa aaagtctcgt ctttaaactt ttgttgctta ggctgcaaaa taaattctta
     1021 tttttccatc tttagtattt tttacctggc atgcaatgaa tttcgtaatg tttgtcacgc
     1081 ggctgtattc ttgcctctta tacctcatta tttgggcttt aggatgcgta atctagctgc
     1141 aaaaaagccg tgctgtgatg gcctgccccc ttagatttag cgatttatgg gggaagtgtt
     1201 tgcgcactca gctcgtggct gcatttgcga ccaatcaagc ccttacaacc atagtttagg
     1261 atgtatttta tgactaaaaa gactttatgt gcctttattg gcctggcggg atttattctt
     1321 gccgcccatt cggcacacgc cgcgcctgtt agtttaaaag tagaaattca acaaaccaat
     1381 gatgacggta gcctggtaaa tggcttaaga accagtaaaa cctgggattt aagcgaggaa
     1441 acactctggc aacctacccc cgaaaacccc gaagtatatg atttgatcac gactgacctt
     1501 aagtggaaaa ataaaagcac tctggctttt ggatccgccg ctgaggttta tgtcaatgat
     1561 ctaaagtatg atgcagaccc gttgttaagt tttgatatta ctcttgcgaa caacactgcg
     1621 tttaaccaaa cctattcact gtcctacaat acgcctttat tacccatgct aaccggagtg
     1681 gtaaatgctt cttcaaattt gactgcagta ttaactgatg tcaatcatct tggcggtgca
     1741 aaaatttctc ctcttgccgg gagcagtatt ttacgtgcct ttgatataac tgtgaatggc
     1801 accgagatac ctaaaaacgt ggatattggc aatagtttta gcattgcaaa cggatcagct
     1861 agcaagaact ggtcagccgt aaatacactg gtgtgtggta cgggtgattt tgcttgcgaa
     1921 accatgtccg ccgtattaac ccttactttg tcaaaaggcg ataaggtgcg gttgtatgga
     1981 gaagtagagc aaattcaagc agtgccagtg ccaaactctg ctgttttatt aggttctatg
     2041 ttggctttat tagcgggcgt gcgacggaaa ttgcgggcgg cttaacaggc tgttgatggc
     2101 tgcgaggcct gcttcgctgc aggatataga atggacaatg ggctgggatg ccctctatta
     2161 aaatgctttt acacg
//
LOCUS       DAOQGS010000673         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33666_length_2175_cov_3.288679, whole genome shotgun sequence.
ACCESSION   DAOQGS010000673 DAOQGS010000000
VERSION     DAOQGS010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33666_length_2175_cov_3.288679"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PKZ52_17600"
     CDS             <1..915
                     /locus_tag="PKZ52_17600"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNG61642.1"
                     /translation="AERRGVYQLANGRAASIDPLHPLSKHTWLVVAETQGSANRSEDQ
                     ILSACPLDPELFTRLLADRVSSQKQALWDKKLGRFTASEQQCIGAIIWQEKRLDALPG
                     EERAQLLVECLRKEGLSYLHWGEETLQLQARVNLAHTQEPERWPLWDEASLLAQLEVW
                     LKPYLNQVNNLQQLHGLNLADILAAQLSWQDQQQLNSLLPTHWQAPTGTQAAIDYTQS
                     PPVLAIKLQEMFGTHTTPSVYQGRINLALHLLSPARRPLQITQDIGGFWRSSYQEVKK
                     EMKGRYPKHPWPDDPLTAPPQRGLKHRS"
     gene            1038..1796
                     /gene="minC"
                     /locus_tag="PKZ52_17605"
     CDS             1038..1796
                     /gene="minC"
                     /locus_tag="PKZ52_17605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNG61643.1"
                     /translation="MRAKTSSSSQAARPESFQIKGGLFPMTLLEVKTDALNTIAEELT
                     ARVAESPAFFNNNPLVLGFEQLKTEQPFDLKSLVALCRELGFIPAAIKGGSSELETLA
                     LGLGLATLPKGKAKGATTLEIDKTPPASKKANRPEIAPPPVFVPPQVIDTPVRSGQQI
                     YTKGDLIILSSVSAGAEVLADGNIHIYGALRGRALAGVQGDESARIFCQSQEAELIAV
                     AGQYVVDEDLRAQHWKSPIQALCANGKLVIAPLF"
     gene            1826..>2175
                     /locus_tag="PKZ52_17610"
     CDS             1826..>2175
                     /locus_tag="PKZ52_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61644.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSSSISSGLALRGHKTAVIDFDIGLR
                     NLDLIMNVERRVVYDFVNVINGEATLKQALIKDKRTENLFILPASQTRDKDALTAEGV
                     ERVLNELSDMGFEF"
BASE COUNT          559 a          611 c          535 g          470 t
ORIGIN      
        1 gctgagcggc gcggtgttta tcaattggcc aatggccgcg ccgccagcat cgacccgctg
       61 catccattga gtaaacatac ctggctggtg gtggccgaaa cccagggctc cgccaaccgc
      121 agcgaagacc aaatcctcag cgcctgcccc ctcgatccgg aattatttac ccgcctgttg
      181 gccgatcgag tgagcagcca aaaacaggcc ttgtgggata aaaaactcgg ccgctttacc
      241 gccagtgagc agcagtgcat cggcgctatt atttggcagg aaaaacgcct ggatgctcta
      301 ccgggcgagg agcgggccca gttattagtt gaatgcctgc gcaaggaagg cctgagctat
      361 ctgcattggg gtgaagagac gctgcaatta caggcgcggg tgaatctagc gcacacacag
      421 gaacccgagc gctggccttt atgggatgaa gcgagtttac tggcccagct tgaggtatgg
      481 cttaaaccct acctcaacca agttaataac cttcaacaat tacacgggct caatttggcg
      541 gatatcctgg ctgcccaact gagctggcag gatcagcagc agctgaattc gctgctacct
      601 acccactggc aagcacctac gggcactcaa gcagccattg attacacaca atcgccgccg
      661 gtgctcgcga tcaagttaca ggagatgttt ggcacccaca caacacccag tgtttaccag
      721 ggccgcatca atctcgcttt gcatctgctc tcgcccgctc gtcgcccgct gcaaattacc
      781 caagatatag gcggcttttg gcgcagcagt taccaagagg taaaaaagga gatgaagggc
      841 cgctacccta aacacccctg gccagacgac cccttaaccg ccccacccca gcggggacta
      901 aagcacagaa gctaaaacaa cccaacaggg gcagaaaaac ccgggcatga tgtacaaata
      961 cttaatccac ttgtgaaatt tcgtgcaaag gtctataaaa tcgccctact caatgcaccc
     1021 cccttccgta gtgtcctatg cgcgcaaaaa cttcctcttc gtcccaagct gctcggcctg
     1081 aatccttcca gatcaaaggc ggcttgtttc ccatgacgct gctggaagtt aaaaccgacg
     1141 ctctaaatac tattgctgag gaactcaccg cacgggtggc agaatcgcca gcatttttta
     1201 acaacaatcc gctggtgtta ggttttgagc aacttaaaac tgaacagccc ttcgatctta
     1261 aatccctggt ggcgctctgc cgcgaattgg gttttattcc ggcggccatt aagggcggta
     1321 gtagtgaact agaaaccctt gcgctcggcc tcggccttgc cactctgcct aagggcaaag
     1381 ccaaaggcgc gaccaccttg gaaatagaca aaaccccgcc agcgagtaaa aaagccaacc
     1441 gcccagaaat cgccccaccg cccgtgtttg tgccgccaca ggtgattgac acgccggtgc
     1501 gctcggggca acagatttac accaaaggcg accttattat tttgtcttcg gtatctgccg
     1561 gggctgaagt gctcgccgat ggcaatattc atatttatgg cgccctgcgc ggccgtgccc
     1621 tggccggtgt acagggcgat gaatcggcgc gtattttttg ccaaagccag gaggctgaat
     1681 taattgctgt ggcagggcag tacgtagttg acgaagacct gcgcgcccaa cactggaaat
     1741 cccccataca agcactctgc gccaatggca aactcgtcat tgcgccgctt ttttaaataa
     1801 ctctaaacag aataggtaaa ttcccttggc caaaataatc gtggtaacct caggtaaagg
     1861 cggtgtgggc aaaaccacca ccagctcatc cattagcagt ggcttggcgt tacgcggcca
     1921 taaaacagca gtgattgatt ttgatatcgg cttacgtaac ctcgatttaa ttatgaatgt
     1981 cgagcgccgc gtggtttacg attttgtaaa tgttattaat ggtgaagcca ccttaaaaca
     2041 ggccctaatc aaagataaac gcaccgaaaa tctttttatt ctccccgcca gccaaacccg
     2101 cgataaagac gctctcaccg ccgaaggtgt tgaacgtgta ctcaatgaat taagcgatat
     2161 ggggtttgaa tttat
//
LOCUS       DAOQGS010000674         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33731_length_2172_cov_7.330184, whole genome shotgun sequence.
ACCESSION   DAOQGS010000674 DAOQGS010000000
VERSION     DAOQGS010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33731_length_2172_cov_7.330184"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(495..1256)
                     /locus_tag="PKZ52_17615"
     CDS             complement(495..1256)
                     /locus_tag="PKZ52_17615"
                     /inference="COORDINATES: protein motif:HMM:NF023011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3238 domain-containing protein"
                     /protein_id="HNG61645.1"
                     /translation="MRALIVIGFSGLSCLALPNPCAATVFHFWIKSFIPNEHPKINNY
                     FKVTSKGTSVLPAPSVQLPIADISKLTDTCFATDNRGFDKSPLASARVTTEMTLVIDE
                     RKVSVEKFNGRDMVRIGQTENVDCKSGEQLSPPRATSPDTVSISGVKQQNFIYVVNIK
                     ASASNPFYKLLGVQMAPDIDYEVVIQYSVLTKSINVDIVTGYFPSFEAYYTLDDGPVT
                     TIINWPPYENSTAASLFNLGLGINTRSVKYKIKLQ"
     gene            complement(1431..1883)
                     /locus_tag="PKZ52_17620"
     CDS             complement(1431..1883)
                     /locus_tag="PKZ52_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61646.1"
                     /translation="MSKLHVNVAVVLALSVYASQSSAGGLFGKGGLIRGDIGNVLDRT
                     IETPVLTPIAKTLAVLPNLAPIATTHTLREAGEAGEAGEAGEAGEEVSEAPSGNEQSV
                     IFSGNKAVMVIPGRDSPADGLNFDQGEFGNAHGVNDFNSNNARIIYEK"
BASE COUNT          557 a          543 c          516 g          556 t
ORIGIN      
        1 atcaagcgcc gctgctacgg catcttcaag cccgagcatc tgttccagcg cctgtgtctg
       61 gaccttgaag gttaccgtct ctacggacgg tcctgaccac aagatgtagg ggtgtccacg
      121 gagacaacgg aagagccaaa aagagtcggc gctggctgtt cgggagtcca cgccgatggt
      181 tcaggagccg gcgtcggttg tcgaagcgtc ggcgccgatg cttgagaaat cgacgccggc
      241 agttcaggag tcggtgccga tcctgcagca cgtaggctgg gttgacgaag gaaacccagc
      301 agtcgttaca tgcgatggca gtgcactggg ctgacgcatt ttttagtcgt ccaggcattt
      361 tttgcttctt cacgctgccc ccgatgctgg gtttcgttcc tcaacccagc ctacgagctg
      421 gttcaggcat tgcgggtatt gtgtcgaggg cggttaataa gcgctgtaat atcacatgtg
      481 attggtataa ccagttactg aagttttatt ttatacttga cagatcgagt attaattcca
      541 agccctaaat taaataggga cgccgctgtc gagttttcat aggggggcca attaataata
      601 gtcgtcactg gaccatcatc caatgtgtaa taagcctcaa atgatgggaa gtaaccagtc
      661 acaatatcaa cattaattga tttagtaaga acactatatt gaatgacaac ctcgtagtca
      721 atatcaggag ccatttgaac accaagcagc ttataaaatg ggttcgaagc tgatgcttta
      781 atgttaacaa cataaataaa attttgttgc ttcacgccac taattgacac tgtgtcagga
      841 ctggttgccc gaggcggact aagttgctct ccggatttgc aatctacatt ttctgtttga
      901 ccgattctaa ccatgtcgcg cccattaaat ttttcaaccg aaacctttct ctcgtcaatt
      961 actagagtca tttcagtggt tacacgagca gaagccaagg gggatttatc aaatcctcta
     1021 ttatctgtcg caaagcatgt gtctgtcagt ttgctaatgt cggcaattgg taactgaacc
     1081 gaaggcgcag ggagtacact cgtgcctttc gacgttactt taaagtaatt gttgattttt
     1141 ggatgttcat taggtatgaa agatttgatc caaaaatgaa aaactgtagc cgcacaagga
     1201 tttggaagtg caagacaaga aagccctgaa aatccaatta ctattaacgc gcgcattttt
     1261 aagcaacctc taatcagacc catcaacaaa tcaccaagtt tcgagtcggc gttctctgtg
     1321 gcgttggtgc atcatcgccg accacatttc aaggccgcgc cggcagctgt catctgccgg
     1381 cggcgggtta acgaaaattc aaacaccaac attggtgtaa taaataattg tcatttttcg
     1441 taaataattc ttgcgttgtt ggagttgaag tcgttaacac cgtgggcatt cccgaattca
     1501 ccttgatcaa aattaagccc gtcggcaggg ctatctcggc caggaatcac cattactgct
     1561 ttatttccac taaaaataac agattgttca ttgcctgacg gcgcctctga aacttcctca
     1621 ccagcctcac cagcctcacc agcctcacca gcctcaccag cctcacgaag cgtatgcgtc
     1681 gtcgctatag gagctaagtt tggaagcact gccagtgttt ttgcaattgg cgtcaaaaca
     1741 ggcgtttcaa tggtgcggtc aagaacattt cctatgtcgc cccggattaa gccgccttta
     1801 ccaaacaatc cacctgcaga agattgactt gcatacacgg aaagcgccaa caccactgcc
     1861 acattcacgt gtaacttgct catgaataat cacctcagtt agtctaagaa tatcattgac
     1921 tttcacgatg ctacagagct acattaagag tgagtatagc cactctgaag atgtggactc
     1981 acgccagaag aaaaagttct tcgggcttct ctgcactcag ccgggtaggg tgcgcatcca
     2041 tgcgcacgcg ttgccgacgg ggctcggctc tgcgagcccc gctccgcctc agcggcaggt
     2101 ccagctcgcc ggatcgtgcc agtcgccggc gccgtccttc agcaccgtgc gctgcgggaa
     2161 gcggcagatc gg
//
LOCUS       DAOQGS010000675         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33776_length_2171_cov_3.481096, whole genome shotgun sequence.
ACCESSION   DAOQGS010000675 DAOQGS010000000
VERSION     DAOQGS010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33776_length_2171_cov_3.481096"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /locus_tag="PKZ52_17625"
     CDS             <1..1612
                     /locus_tag="PKZ52_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG61647.1"
                     /translation="MKRQLIAPSITTQLSERAYVEVNSLWQHNTQTAATDFKIPATNT
                     TQDTFYQPYPERSAQFDSRFQLTNARYRFDITPNWSINLNAGHIKEKRQGVRPSSDTL
                     TNGDVLLKQPVGEDTLIIPLGGRVAVPLNLRQSGADWTFQVGAIHSLYAEADLETNQQ
                     AALLCNGAIDTNHWQHKMTFGLEWRKQNLSKTLLAEVNDYYPGRTWRLNSYNAVLNDI
                     TARLFAANRTLGNLEPQATQLINADTSLLLTDSITLTDNWIASLGSRYSRMSGDLTYS
                     EFSAAPSQQYPLPSFDNLSSQFGLVYKPSETHSWYFNYSEAVRANYRIDAPEAKNAQP
                     ETSSQYEIGFKSLAFDSRLLMSLGFYRIAKENISEIIPKRGALNSLNYFDQSAQGLDV
                     DMSWQISQQWDVMAGIGYVDALVESGKQQGKTPADIAKNSASLFTHYRFNNHWSADAG
                     LSYLGPRFGSTLGDKLDVLGQKAKLPAYTTLDLHLNYHYKLWDLPSEIKLSIKNATNE
                     QYYTAFVAGVRPNVAEGRSILGTLRLTY"
     gene            1612..2124
                     /locus_tag="PKZ52_17630"
     CDS             1612..2124
                     /locus_tag="PKZ52_17630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNG61648.1"
                     /translation="MRHAHPLALLFQLHYADIRAQIAAKFRRPPQETEDIVQDAFHNI
                     LRANNLEALENPKAYLYKTASNLALNRLRQHRLNAQAIDTQEAPMHAEQDEISPERQV
                     LAQQDLARLYTALNSIPEKYRRTFLLSRMHNKTYKEISEQLNIAESTVEKHIIKVLKC
                     LREHLQEDCS"
BASE COUNT          619 a          571 c          480 g          501 t
ORIGIN      
        1 tatgaagcgg caattaattg caccttccat cacgacgcag ttaagtgagc gagcgtatgt
       61 ggaggttaat tccctgtggc aacacaatac tcaaactgcg gctaccgact ttaaaattcc
      121 cgccaccaac accacacaag atacgtttta tcagccgtat cccgagcgca gcgcgcaatt
      181 tgacagccgc tttcaattaa ccaatgcacg ctaccgcttt gatataacac ctaactggtc
      241 aatcaacctc aatgcaggcc acataaaaga aaaacgccag ggcgtgcgcc ccagcagcga
      301 tacactcacc aatggtgatg tattgcttaa acaacctgtt ggcgaggaca ctttaattat
      361 ccccctcggg gggcgtgtag ccgtgccttt aaacctgcgt caaagtggcg ccgactggac
      421 ttttcaagtg ggtgccatcc acagcttata tgcggaggca gacttagaaa ccaatcaaca
      481 ggcggcgctg ctgtgcaatg gcgcaatcga tacaaaccac tggcaacaca aaatgacgtt
      541 cggcctggaa tggcgcaaac agaatttatc aaaaactcta ttggccgagg tcaatgatta
      601 ttacccaggg cgaacctggc ggcttaatag ttataacgct gtgttaaacg atattactgc
      661 tcgcttattt gccgccaacc gtacactcgg gaatttagaa cctcaggcca cacaattaat
      721 taacgctgat acaagcctat tattaactga ctccatcaca cttaccgaca attggattgc
      781 ctccctcggc agccgttaca gccgcatgag tggcgattta acctacagtg aattcagcgc
      841 ggctccatca cagcaatatc cgctgccaag ctttgacaat ctttcctccc aatttggcct
      901 ggtttataaa ccctcagaaa cccattcttg gtactttaat tacagtgaag cggtgcgcgc
      961 caattatagg atcgacgcgc ctgaagctaa aaatgcccag ccggaaacct ccagccaata
     1021 cgagataggt tttaaatctt tagcatttga tagccgttta ttaatgagct taggttttta
     1081 tcgcattgcc aaagagaata tttctgaaat tattcccaag cgcggcgcac tcaattcgct
     1141 taattatttc gaccaatcgg cgcagggact ggatgtggat atgagctggc aaataagcca
     1201 gcagtgggat gtgatggcag gcataggtta tgtggatgcg ctcgttgaat ccggcaagca
     1261 acagggcaaa actccggcgg acatagccaa aaactccgcc agtttattca cccattatcg
     1321 ttttaataac cactggtcgg cagacgcagg cctgagttat ttaggccctc gctttggtag
     1381 caccctgggt gataaattag atgtgctggg gcaaaaagcc aaactgcccg cttacaccac
     1441 cctggatcta caccttaact atcactataa actctgggat ctgccgtctg aaattaagtt
     1501 atccattaaa aacgccacca atgagcagta ttacaccgca tttgtggcag gcgttcgacc
     1561 gaatgtcgcc gaagggcgca gcattttagg cacacttagg ctcacctact gatgcggcac
     1621 gcccatccac tcgcgttatt atttcagctg cattacgccg atatccgcgc gcaaattgcc
     1681 gccaagtttc gccgtccgcc acaggaaact gaagacatag tgcaggatgc cttccacaat
     1741 attttgcgcg caaataatct tgaagcgctg gaaaacccaa aggcgtattt atacaaaacc
     1801 gccagcaatt tagcccttaa tcgcctgcgt caacatcgtc tcaatgcgca ggcaatagac
     1861 acccaagaag cgccgatgca tgcggaacag gatgaaatat cgccggagcg acaggtgtta
     1921 gcccagcaag atttagcccg gctctataca gcactcaaca gcattccgga aaaataccgg
     1981 cgcacttttt tattaagccg tatgcacaat aaaacctaca aagaaattag cgagcaactt
     2041 aatatcgccg agagcaccgt cgaaaaacac attattaaag tgttgaaatg cctgcgggag
     2101 cacctacaag aggattgcag ctaatggcta aacaggtctc tgtagcggac gaccaggcgc
     2161 tggcgctgat c
//
LOCUS       DAOQGS010000676         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_33855_length_2168_cov_5.166115, whole genome shotgun sequence.
ACCESSION   DAOQGS010000676 DAOQGS010000000
VERSION     DAOQGS010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33855_length_2168_cov_5.166115"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(670..888)
                     /locus_tag="PKZ52_17635"
     CDS             complement(670..888)
                     /locus_tag="PKZ52_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HNG61649.1"
                     /translation="MSIKEIKAFSAKAKEDSELGAKLKSCEKLRELMALAKDSGFDVK
                     EELFYPPNEPQFTEEQLSLKLANALLRV"
     gene            complement(1385..>2168)
                     /locus_tag="PKZ52_17640"
     CDS             complement(1385..>2168)
                     /locus_tag="PKZ52_17640"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNG61650.1"
                     /translation="YRWPIIDEVATACEKPRTQAQQVAYPQLPPPLALECREPAARLL
                     RQRRSAQAFDRTTPIALQDFFALLDHLLPRQHLPPWDCLESAPAIHLVLFVHRVEGLA
                     CGLYALARSQEGLQLLQEEFKREFSWQKVTSAPDHLPLYHLITGKAERMAARLACQQA
                     IASDSAFALAMLAEFSGPLSQSPWRYNQLYQEAGLIGQSLYIDAEAIGLRGTGIGCFF
                     DEGVHEVLGINSEALQSLYHFTLGGPIIDTRIISLPPYPERT"
BASE COUNT          490 a          466 c          594 g          618 t
ORIGIN      
        1 accgcgaatc gattatgccg gatggctaaa tcctcctgaa tttgccgccc gcgcgaaaaa
       61 cgggaggaaa gtgtgctgag tgtgtgctga gtctgtgcag aatagttacg ataattcttc
      121 ttttcaagaa tcttcatttt ttattgtctg tgcatcttat gcatgcaaac tccctaacaa
      181 tctttttggt aactatttag cttgggtgat tactctcgcc acgtggggga cgagtttagg
      241 ggcctcaagc ggcattctcg attcgcggtg cagtggtggt gaatacagta tttcagactg
      301 tcaaaaaaat ggaatttttt gacgagacta catggatgta ttcacggcgt gtctgaaata
      361 agctgtattc atcactgctg gggagtattg tagacgttca gcactagagc gctcgcttga
      421 ttgagggcgt ttcatttaaa gtccagcatt caatctgcac gggtatttca ccctcgcagt
      481 taaaaactgc ctcttccgct cttgaatatt gggatatttt ctcgtcctta gcgattaaaa
      541 ttgcttacac agcaattgcc tttagcctta gcttatcagg ctaatcgtgc tcgaaactgc
      601 agaatttaaa acacgccaaa agcaagcagc ccaatgattg agctgcttta agtattttga
      661 acaatgtggt taaacgcgca gtaaagcatt ggctaatttt agtgacaatt gctcttcggt
      721 aaattgcggc tcattgggtg ggtagaagag ttcttcttta acgtcaaagc cgctatcttt
      781 tgccagtgcc ataagttcgc gcagcttttc gcaggatttt aatttggctc ccagttctga
      841 atcttctttt gctttcgcag aaaaggcttt gatttcttta atcgacataa cggatgctcc
      901 agtaacatga tgtattctct aacgcccagt ggtagcagct tcgtgtaggg cttgccacag
      961 actcgtgggc gtatcagcaa taaataggcc acggggttgc gcgcagattg tgtgctttaa
     1021 accccgaaat ttagtgactt tgtgcgggtt gtttaattct ggcgtaacac aatttggtgg
     1081 tgtttacgac aaaactaaca acttttaacg gcgcggcaat agttgtcggc tttttgtagg
     1141 tttcgttggg tgttaataat ctgtatcggg ggcgatatgt atttgagcgc tcgacccatt
     1201 aattttattt ttgctcaatg cgcttggttt tattacatgg cttgattttc ttgcatgact
     1261 tgggttttat tgcatgactt aggttttatt gcacgacatt aaattgcctg gatataaaac
     1321 ctattgccag ggcttgcgtg tgtgctggaa caatagatca tttaggcttg agattagccc
     1381 ttgactaagt gcgctctgga taggggggca ggctgataat gcgggtatcg ataattgggc
     1441 cgccaagggt gaagtgataa aggctttgca gcgcctcgct gttgatacct aaaacttcat
     1501 gaacgccttc atcaaaaaaa caacctatgc cggttccccg caggcctatg gcctctgcgt
     1561 ctatatagag ggattggcct atgaggcctg cctcctggta gagctggttg tagcgccagg
     1621 ggctttggct taaggggccg ctaaactccg ccagcatggc tagggcaaag gcgctatcac
     1681 tggcaatagc ctgttggcag gccaggcggg ccgccatgcg ttcggctttg ccggtaatca
     1741 gatggtagag gggcaggtgg tccggggctg aggttacttt ctgccagcta aattcccgtt
     1801 taaattcctc ttgtaaaagc tgcaggccct cctggctgcg cgctagggcg tagaggccgc
     1861 aggcgagtcc ctccacgcga tggacgaata ggactaaatg tattgctggg gccgattcca
     1921 ggcagtccca agggggcagg tgttggcgcg gcagcaggtg gtccagcagt gcaaaaaagt
     1981 cttgcagggc tatgggggtt gtgcggtcga aagcttgggc tgagcggcgc tggcgcagta
     2041 gacgtgcggc aggttcacgg cactctaagg ccaggggggg aggtagctgc gggtaggcaa
     2101 cctgctgggc ttgcgtcctg ggtttttcgc acgcggtggc cacttcatca ataatgggcc
     2161 agcggtaa
//
LOCUS       DAOQGS010000677         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34031_length_2162_cov_3.130517, whole genome shotgun sequence.
ACCESSION   DAOQGS010000677 DAOQGS010000000
VERSION     DAOQGS010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34031_length_2162_cov_3.130517"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKZ52_17645"
     CDS             <1..894
                     /locus_tag="PKZ52_17645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG61651.1"
                     /translation="AAANSFNQMLLVATTVFGLSFSANAQLMAQTREFADAQLPANQA
                     TLFNSVVRACIAVAWIAGPPIGFILLGQLGFNLQCKIVGSAYFATALLCLWVLPKTGK
                     SQVPATQVTRSKQKLGPTMLGGIIAFSLMFGCNASYQIALPLMIRDKLQADPALAGFM
                     MGTAALLEIPLMILAGWLGSRRPLLPLIRIGAAAAVVLYLGIWQATAVWQLFALQLLN
                     AVFIGFIAGLGMTWFQNLLPGQAGLSSSYFSNANGLGEITGYLIIALVAQRIGYGDMY
                     GFTGLVALLALVVLSTVCKKA"
     gene            complement(939..>2162)
                     /locus_tag="PKZ52_17650"
     CDS             complement(939..>2162)
                     /locus_tag="PKZ52_17650"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNG61652.1"
                     /translation="PVLRAHLPIWQAELRQLATWWSNVARDPQGGFYGEVGLDNQPLA
                     SAPRGLIHNARILYFFSRLARHTLDAEHSACAHHAYEYLAAHFYDAAHGGYFGLLDAE
                     GRPLDKHKHLYAQAFALYGLTTYYQLTGRAEVLAQALELFSRLETVAADSQWGGYWEG
                     FAADWRWLEDGRLCETSPQAAKTANTHLHLLEAYTALAQASGETSVRQSLARLLKVFC
                     VRLVDEPGGHLRLFLTRAWQDCSGQISFGHDIEASWLLQSALHAAGDETLLQRYAPLA
                     QRLAEVSLNTAWSPRGGIRDGQSLTGGAYAEERTWWAQAEAFIGYLYAWQRSADSRFA
                     ERAIATWQFIYPQHRDERLGEWFWFAKDDVKPPLSAYQLSRNYKSGFWKCPYHNGRAL
                     IEACELMANIKTAPP"
BASE COUNT          436 a          646 c          624 g          456 t
ORIGIN      
        1 gccgccgcca actcctttaa ccagatgttg ctggttgcca ccacggtgtt cggcctgtcg
       61 ttctcggcta acgctcagtt aatggcgcag acccgcgagt tcgccgatgc ccaactgccc
      121 gccaaccagg ccaccctgtt taattccgtg gtgcgcgcct gtattgccgt ggcctggatc
      181 gcagggccgc cgatcgggtt tattctgctg ggccagttag gttttaacct gcaatgcaaa
      241 atcgtcggca gcgcctattt cgccaccgcc ctcttgtgcc tctgggtgct gccgaaaacc
      301 ggtaaatccc aagttcccgc cacccaggtg acacgttcaa aacagaagct cgggccgact
      361 atgctcggcg gtatcatcgc ctttagcctg atgttcggtt gcaacgccag ctaccaaatc
      421 gccctgcccc taatgattcg cgacaaactc caggcggacc cggccttggc gggtttcatg
      481 atgggcaccg cggccctgtt ggaaattccc ctgatgatac tcgccggttg gctcggcagc
      541 cgaaggcccc tgctgccctt gattcgcatc ggcgctgccg ccgcggtggt gctgtacctg
      601 ggtatttggc aggccacggc ggtgtggcaa ttgtttgcgc tgcagttatt gaatgcggtg
      661 tttatcgggt ttattgcggg cctgggcatg acctggtttc aaaacctatt gccggggcag
      721 gcggggctta gctcgtcgta tttcagtaac gccaatggct taggggagat taccggttat
      781 ttaattattg ctttggtcgc ccaacgcatt ggttatgggg atatgtacgg atttaccggc
      841 cttgtggccc tgctggcgct agtggtttta tcaaccgtgt gtaaaaaggc gtagcgggca
      901 atgcccgccc tgggaaaaca ggcctgtaaa acagccgttt aaggcggcgc cgtttttata
      961 tttgccatta attcacaggc ctcaataagg gcgcggccat tgtggtaagg gcatttccaa
     1021 aagccggatt tatagttgcg ggacagctga taggcgctca aaggcggctt tacatcatcc
     1081 ttggcaaacc aaaaccactc gcccaagcgc tcatcccggt gctgcggata aataaactgc
     1141 caagtagcaa tggcccgctc ggcaaagcgg ctgtcggcac tgcgctgcca cgcgtatagg
     1201 taaccaataa acgcctccgc ctgagcccac caggtgcgct cttctgcata ggcgccgcct
     1261 gtcaggcttt gcccatcgcg aataccaccc cgtggcgacc aagctgtgtt gaggctcacc
     1321 tcagccaagc gctgagccaa gggggcatag cgctgtaaca gtgtttcatc tcccgctgca
     1381 tgtaacgcgc tctgcaacag ccaactggct tctatgtcgt ggccgaagga gatctgcccg
     1441 gagcaatcct gccaggcgcg cgttaaaaat aggcgcaggt ggccgccagg ttcatccacc
     1501 aagcgcacac aaaacacctt aagcaaccga gccaggctct gccgcaccga tgtttccccg
     1561 ctggcctgtg ccagagcggt gtaggcctcc agcagatgca ggtgggtatt cgccgttttg
     1621 gcagcttggg gcgaggtttc gcacaggcgc ccatcctcaa gccaccgcca atcggcagca
     1681 aaaccttccc aataaccgcc ccactggcta tccgccgcaa ccgtttccaa gcgcgagaac
     1741 aactccagag cctgggccaa cacctcagcc cgaccggtta attggtaata ggtggttaag
     1801 ccgtagaggg caaacgcctg ggcgtaaagg tgtttgtgtt tatccagggg gcggccctcg
     1861 gcatccagca ggccaaaata gccgccatgg gcggcgtcat aaaaatgcgc ggccaggtac
     1921 tcataggcgt ggtgggcgca cgcagagtgc tcggcatcca gcgtgtggcg ggcgaggcgg
     1981 ctgaaaaaat acaaaatgcg ggcgttgtga atcagccccc tgggagcgct cgccaggggt
     2041 tgattatcaa gccccacctc accgtaaaaa ccaccctggg gatcgcgggc gacattgctc
     2101 caccaggtgg ccagttgccg tagttccgcc tgccagatgg gcagatgcgc cctgaggacc
     2161 gg
//
LOCUS       DAOQGS010000678         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34250_length_2154_cov_3.191996, whole genome shotgun sequence.
ACCESSION   DAOQGS010000678 DAOQGS010000000
VERSION     DAOQGS010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34250_length_2154_cov_3.191996"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PKZ52_17655"
     CDS             <1..1287
                     /locus_tag="PKZ52_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNG61653.1"
                     /translation="DVNMGRLSGHDLLRLVKQDYPHIPVLLITAYASISESVQAMRDG
                     AVDYLVKPFAPDLLISTVKHFIGAGLVDLDEPIAMAESSRQLLQLAKRIAQSDSTALL
                     LGESGTGKEVLARYIHNHSPRAHMPFIAINCAAIPDNMLEAMLFGHEKGAYTGAHSAS
                     PGKFEQANGGTLLLDEISEMELGLQAKLLRVLQEREVERLGGRKSIKLDVRVIATSNR
                     DMHREIAAGKFREDLYFRLSVLPLQWAPLRERVEDIVPLAQALLNRHAIKQHRLAMQL
                     SASAKQALMAYSWPGNVRELDNVMQRALILQTGSVIEDRDLGLQMYNNASAKPAHGDF
                     IRSGQEFNRPQSAADIPVSFTAARARQLIAEEDEGEIAGLGSDLRQREYEIIMETLKK
                     EHGSKKNTADRLGISPRTLRYKLARLRDEGFCFERA"
     gene            1841..>2154
                     /locus_tag="PKZ52_17660"
     CDS             1841..>2154
                     /locus_tag="PKZ52_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61654.1"
                     /translation="MSAAKLILLAFGAAILAGCSGEGSSSSSSSSSSSSSSSGKSSSS
                     SSSSSGKSSSSSSSSSGKSSSSSSSSSSSSGRTSSSSSSSSSSSSSSSTSSSSSSGST
                     RVV"
BASE COUNT          531 a          532 c          538 g          553 t
ORIGIN      
        1 gatgtgaata tggggcgctt aagcggccac gatttattgc gcttggttaa acaggattat
       61 ccgcatattc ccgtgttatt aattaccgcc tacgccagta ttagcgaatc ggtgcaggcc
      121 atgcgcgatg gcgcagtgga ttatttagta aaaccctttg cgcccgatct gttaattagt
      181 acggtcaaac attttattgg cgcaggcctc gtggatttgg atgagccgat tgccatggcc
      241 gaatccagcc gccaattatt gcagctggct aagcgcatag cgcaatcgga ctccaccgct
      301 ttattgttag gtgagtccgg cacaggtaaa gaagtgttgg cgcgctatat acacaaccat
      361 tcgccccgcg cccatatgcc atttattgcc attaactgcg ctgccatccc cgataatatg
      421 ctcgaagcca tgttattcgg ccatgaaaaa ggcgcttata ccggcgccca tagcgcatcg
      481 cctggtaaat ttgagcaggc caacggcggc actttattgc tggatgaaat ttcggaaatg
      541 gaattaggcc tacaggctaa attattgcgc gtactgcaag agcgcgaagt ggagcgtttg
      601 ggtgggcgaa aaagtattaa actcgatgtg cgcgtaatcg ctacttcaaa ccgcgatatg
      661 caccgagaaa tcgccgctgg taaattccgc gaagatttat attttcgcct cagtgtgttg
      721 cccttgcagt gggcgcccct gcgggagcgg gtggaggata tagtgccctt ggcccaagcg
      781 ctgttaaacc gccacgccat taaacaacac cgtttagcca tgcaattatc agcttcagct
      841 aaacaggcgt tgatggctta tagctggccg ggcaatgtgc gcgagctgga taacgttatg
      901 cagcgggcgt taattttaca aaccggcagt gtcattgagg atcgggattt aggtttgcaa
      961 atgtacaaca acgccagcgc aaaacctgcc catggggatt ttatccgctc cggccaagag
     1021 tttaatcggc ctcagtcggc tgccgatata ccggtttcgt ttacagcggc gcgggctcgg
     1081 caattaatag cggaagagga tgaaggcgag attgccgggc tcggcagcga tttgcgccag
     1141 cgtgagtatg aaataattat ggagacatta aaaaaagagc acggtagcaa aaagaataca
     1201 gcggaccggc tgggtattag cccgcgcaca ttgcgctata aattagcgcg tttgcgcgat
     1261 gagggttttt gttttgagcg ggcctagggg cgcaaactct tggtgactca gcgtaaagtt
     1321 gtattcaccc ttactacact gcaaattgct tatgtctctt ggctaaatct ctcaatctgt
     1381 gcgctggcgc gaagaggaga ggagagcgca ataaagcctg ctcaatagtg ataaatttct
     1441 atggtcggcc cgttgccgcc caactactac ctagacaccc gcaatgaata cacatcattt
     1501 ctgacacgcc gtgaatacat ccgtgtaggc tcgtcaaaaa aatccctctt tttgacggtc
     1561 tgaaatgatg tgtattcatt gcttccgcac agctagtaat tcaactggcc ggaacttgtt
     1621 taaccgcccg ctgaattagg tctgtcgggc aggcagcggt aaatggaaaa aggcggcgca
     1681 ggcaattgat aaattaatgt tatcgtactt tttactactt aaaattgaat agaattatct
     1741 agctgtcgag ggatcaatag tcacagagtg acttttgtca tatcacggtt ttacaatctc
     1801 cgtacaatgg cccctataaa tcgaatcatt aggggcatcc atgtctgcag cgaaattaat
     1861 cttactggcg tttggcgccg caattctggc gggctgcagt ggtgaaggca gcagttcatc
     1921 cagcagttcc agttcttcct cgtccagttc atcaggtaaa tccagcagtt cttcttccag
     1981 ttcatcgggc aaatccagca gctcttcctc cagctcctcc ggtaaatcat ctagctcctc
     2041 aagctcctcc tcctcaagtt cgggccgaac ttcatccagc tccagtagtt catccagttc
     2101 cagtagttcc tcctccactt cctccagctc aagctcaggt tcaacccgtg tggt
//
LOCUS       DAOQGS010000679         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34290_length_2152_cov_4.315212, whole genome shotgun sequence.
ACCESSION   DAOQGS010000679 DAOQGS010000000
VERSION     DAOQGS010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34290_length_2152_cov_4.315212"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="rfbA"
                     /locus_tag="PKZ52_17665"
     CDS             complement(<1..805)
                     /gene="rfbA"
                     /locus_tag="PKZ52_17665"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNG61655.1"
                     /translation="MRKGIILAGGSGTRLYPVTLAVSKQLLPIHDKPMIYYPLSTLML
                     AGIRDILIISTPQDTPRFSQLLGDGSQWGINLTYAVQPSPDGLAQAFIIGRDFIGTDT
                     SALVLGDNIFYGHEFADDLSKAAVDQNGARVFAYHVHDPERYGVVEFDKTGKAISLEE
                     KPKQPKSNYAVTGLYFYDNQVVDIAKDLTPSPRGELEITDVNRIYLQRNQLAVQVMGR
                     GHAWLDTGTHESLLDAGQFIATIEKRQGLKIACLEEIAFLNKWIDAAQLE"
     gene            complement(815..1708)
                     /gene="rfbD"
                     /locus_tag="PKZ52_17670"
     CDS             complement(815..1708)
                     /gene="rfbD"
                     /locus_tag="PKZ52_17670"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNG61656.1"
                     /translation="MTRILLTGKNGQVGFELQRALAPLGEVLALDRRGFDLHNEANMR
                     DVIKQFAPNIIVNPAAYTAVDKAQSEPELAQAINAHALAVIGEEAAKLNALVVHYSTD
                     YVYDGHKPDPYEETDTPAPQSVYGKSKLAGEQALINSQAAHLIFRTSWVVGAYGNNFA
                     KTMLRLAAERDGLRVVADQFGAPTSAALIADVTAQVLGQYLNRGDKTFPQGVYHLAAA
                     GETTWHAYAKWVIGRAIEAGRTLKTQPEEVEAIATSAYPLPAPRPLNSRLNTQRLCRD
                     FGLTMPPWQQGLEHVMRLILL"
BASE COUNT          541 a          507 c          519 g          585 t
ORIGIN      
        1 cttctaattg cgcggcgtca atccatttat ttaaaaaggc aatctcctct aaacaggcga
       61 tttttaggcc ttggcgtttt tcaatggtgg caataaattg cccggcatca agcagggatt
      121 catgggtgcc tgtatccagc cacgcgtggc cccggcccat tacttgtacg gcaagttggt
      181 tgcgctgcaa ataaatgcgg tttacatcgg taatttctaa ttcaccgcgt gggctggggg
      241 tcaagtcttt agcaatatcc accacttggt tgtcgtaaaa atacaaaccg gttacagcat
      301 aattggattt gggctgcttg ggtttttcct ccaaactaat ggctttgccg gttttatcaa
      361 attccactac accgtaacgc tctgggtcgt ggacgtgata ggcgaatacg cgggcgccgt
      421 tttgatccac cgctgctttt gataaatcat cggcaaattc gtggccataa aaaatattat
      481 cgcccaatac taacgccgaa gtatccgtgc cgataaaatc ccgaccgata ataaatgcct
      541 gggctaagcc atctgggctt ggctgcacag cataggttaa attaatgccc cactggctgc
      601 catcgcctaa taattgacta aaacgcggtg tatcttgcgg ggtggagata attaaaatat
      661 cgcgaatacc cgccagcatt aatgtgctta aggggtaata aatcatgggt ttatcgtgaa
      721 tgggtaataa ctgtttggaa acagctaagg tgacgggata taaacgggtg ccggaacctc
      781 cggcgaggat aatacctttg cgcattgtgc ctccttaaag caaaatcaaa cgcattacat
      841 gctctaaacc ctgttgccag ggcggcatgg ttaaaccaaa atcccggcat aatcgctggg
      901 tatttaaacg agaatttaat ggccgtggcg caggtagtgg gtaggcgctg gtggcaattg
      961 cctctacctc ttctggctga gtttttaatg tgcgcccggc ttcaattgcg cggccaatta
     1021 cccatttagc gtaggcgtgc caggtggttt ctcccgcagc ggccaaatgg taaacccctt
     1081 gggggaaggt tttatcgccg cggttgaggt attggcctaa cacctgggcg gttacatcgg
     1141 caattaatgc tgcagaagtg ggtgcgccaa attgatcggc cactactcgc aaaccatcgc
     1201 gctcagccgc taaacgcaac atggttttag caaaattatt cccgtaggcg cccaccaccc
     1261 aactggtacg gaaaattaaa tgggccgctt gagaattaat tagcgcttgc tccccagcta
     1321 acttactttt gccgtaaacg ctttgggggg ccggggtatc cgtttcttca taagggtctg
     1381 gtttgtggcc gtcgtacaca taatccgtag aataatgcac caccaacgca tttaatttgg
     1441 cggcctcctc ccctattaca gcaagtgcat gggcattaat ggcctgggct aattccggct
     1501 cggattgggc tttatccacg gcggtatagg ccgctgggtt gacgataata ttaggtgcaa
     1561 attgtttaat tacatcgcgc atattggctt cgttgtgtaa atcaaagcct ctgcgatcca
     1621 acgctaacac ttcccctaaa ggcgctaaag cgcgctgcaa ttcaaagccg acttggccgt
     1681 tttttccggt gagtaaaatt cgtgtcatag tgggtcttaa gggtaatgag tagttaaatt
     1741 atttaattat tagcctaggt ggcaatgcta cttatttaca atatattcga ggtgtacatt
     1801 taatttccat taagtggcaa tggtattttt aaggtttgtg cgttgtttga tctcacctaa
     1861 aaccccctcc cagcctcccc cttaaaaagg gggaggagcc gaaaacctac aaatttaagc
     1921 atagagctag tgccctaggt gtttttgcgc cccacttttt ttaaggagag gcttgggagc
     1981 gccctagctc aatattattg ggtaaaacta aaaacatgct ctggtgtagg ttggaagagc
     2041 ccgacatcta tcccaccact ataaactccg ggcagagcaa agctctcagc gaacatcatg
     2101 tcggaagtcg ctcggcaatt gctcctgcat tgctctacta tgtcacatcc at
//
LOCUS       DAOQGS010000680         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34292_length_2152_cov_3.919409, whole genome shotgun sequence.
ACCESSION   DAOQGS010000680 DAOQGS010000000
VERSION     DAOQGS010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34292_length_2152_cov_3.919409"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2152)
                     /gene="aceE"
                     /locus_tag="PKZ52_17675"
     CDS             complement(<1..>2152)
                     /gene="aceE"
                     /locus_tag="PKZ52_17675"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNG61657.1"
                     /translation="ATLYDVGFNYFFRGNDGEQPGDLIYFQGHSSPGMYARSYLEGRI
                     SEQQLDNFRREVDGNGLSSYPHPWLMPDYWQFPTVSMGLGPINAIYQAHVMRYMSARG
                     LSARGDRKVWAFLGDGECDEPETLGAISLAGREQLENLIFVINCNLQRLDGPVRGNGK
                     IVQELEGVFRGAGWNVIKVLWGSKWDPLLAKDKTGLLQKRMDEVVDGELQNYKANGGA
                     YTRQHFFGKYPELLELVKDMTDDEIMHLNRGGHDPQKVYAAYAAAMGHKGQPTVILAQ
                     TVKGYALGTAAESANVAHSVKKLDIESLKRFRDRFGIPVADEDLKKVPYYRPAADSPE
                     MQYLHKTRKALNGYVPSRVKDFTPLQIPSLDAFAALLKSTGEREISTTMSFVRFLSTL
                     VKDKNMGDKVVPIVPDEARTFGMEGMFRQLGIYSSEGQKYVPHDSDQIMFYKEDKRGQ
                     ILEEGINESGAMSAWIACATAYSTYRVPMVPFYIYYSMFGFQRIGDLAWLAGDIQARG
                     FLIGATAGRTTLNGEGLQHQDGHSHILANTIPNCRSYDPTYGYELAVIIQDGMKRMYE
                     NKENCFYYITTMNENYVHPDMPAGAEAGIVRGLYKLHESKKKAKKKVQLLGAGTILRE
                     VEAAAELLRENHGVEADVWSVTSVNELTRDAQRAERWNRYHPTEKPKKPYVTSTLEGS
                     DGPFIISTDYMKNYSEQLRAYIPGDYYVLGTDGFG"
BASE COUNT          513 a          551 c          540 g          548 t
ORIGIN      
        1 gccgaagccg tcggtgccca gcacgtaata atcaccgggg atgtaagcgc gcagctgttc
       61 actgtagttt ttcatgtagt cggtggagat gataaacggc ccatcgctgc cctcaagggt
      121 gctggtcacg tagggttttt tcggtttttc ggtggggtga tagcggttcc aacgctctgc
      181 ccgttgggca tcgcgggtca gctcattcac cgaggttacg ctccacacat ccgcttctac
      241 gccgtggttc tcgcgcagca attcagctgc cgcttccact tcgcgcaaaa tagtgccagc
      301 gcccaacaac tgcacttttt tcttcgcttt tttcttgctc tcgtgcagtt tgtacaggcc
      361 gcgcactatg cccgcttcgg caccggctgg catatcgggg tgaacgtagt tttcattcat
      421 ggtggtgatg taataaaaac aattttcttt gttttcatac atgcgcttca taccgtcctg
      481 aataatgacc gctaattcgt aaccgtaagt tgggtcgtag gagcggcagt tgggaatggt
      541 attggccaga atatggctgt ggccatcctg gtgttgcaag ccctcaccat tgagcgtggt
      601 gcgcccggcg gtggcgccga tcaagaaacc gcgcgcttga atatccccgg ccagccaagc
      661 caaatcgccg atgcgctgga agccaaacat ggagtaataa atgtagaacg gcaccatggg
      721 cacccggtag gtggagtacg ccgtggcaca ggcaatccag gcagacatag cgccggattc
      781 attaataccc tcttctaaaa tctgaccgcg tttatcttct ttgtagaaca taatttggtc
      841 ggaatcgtgg ggcacatatt tttgcccttc cgaagaataa atacccaatt ggcggaacat
      901 accttccata ccgaaggtgc gcgcttcatc cggcacaatc ggcaccactt tatcgcccat
      961 atttttatct ttaactaggg tcgataaaaa ccgtacaaac gacatagtgg tggaaatttc
     1021 ccgttcaccg gtggatttaa gcagtgctgc aaaagcatcc aaactgggaa tttgcagcgg
     1081 cgtaaaatct ttcacgcggc tgggcacata accgtttaaa gctttgcggg ttttatgcaa
     1141 gtactgcatt tccggtgaat ccgccgcagg gcggtaataa ggcacttttt tcaaatcttc
     1201 atcggcgaca ggaataccga agcgatcgcg gaaacgcttt aagctttcga tatcgagttt
     1261 tttcaccgag tgggccacgt tggccgattc tgccgcagtt cccaaggcat aacccttaac
     1321 ggtttgggca agaattacgg tgggctggcc tttatggccc atagccgcag cgtaagctgc
     1381 gtagactttt tgtggatcgt ggccgccgcg atttaaatgc ataatttcat cgtcggtcat
     1441 atcttttacg agttctaaca actcaggata tttaccgaag aaatgttgac gggtataggc
     1501 gccgccattg gctttgtaat tttgcaattc accgtctacc acttcatcca tgcgtttttg
     1561 caacaggccg gttttatctt tggccagcag cggatcccac ttgctgcccc agagtacttt
     1621 aataacgttc cagcctgcgc cgcggaagac gccttccagt tcttgcacaa ttttgccatt
     1681 gccgcgcaca gggccatcta agcgctgtaa attacagttg ataacaaaaa ttaaattctc
     1741 taactgctca cggcccgcca gagaaatagc gccgagtgtt tcaggttcat cacactcacc
     1801 gtcgcctaaa aaggcccaca ctttacgatc gccacgggcg gataaaccgc gtgccgacat
     1861 ataacgcatg acgtgggctt gataaattgc attgataggg cctaagccca tagaaactgt
     1921 tggaaattgc cagtaatcgg gcattaacca gggatgtgga taagaagata agccgttgcc
     1981 gtccacttca cggcggaaat tatccaactg ctgttcggaa attcggcctt caaggtagga
     2041 gcgcgcgtac ataccgggcg agctgtgacc ttggaaataa attaaatcac cgggctgttc
     2101 gccgtcgtta ccgcggaaaa aatagttaaa gccaacatcg tacagggtgg cg
//
LOCUS       DAOQGS010000681         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34318_length_2151_cov_3.652195, whole genome shotgun sequence.
ACCESSION   DAOQGS010000681 DAOQGS010000000
VERSION     DAOQGS010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34318_length_2151_cov_3.652195"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKZ52_17680"
     CDS             complement(<1..295)
                     /locus_tag="PKZ52_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNG61658.1"
                     /translation="MSKIGIFFGTETGTTRLIAKKIHKKLGDDLADKPLNVNRISVDE
                     LLKYDFLILGTPSYGVGDIPGRSAGCIETNWEEFLTTAGKPDFTGKKIALFGLG"
     gene            complement(308..697)
                     /locus_tag="PKZ52_17685"
     CDS             complement(308..697)
                     /locus_tag="PKZ52_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61659.1"
                     /translation="MNTSNQQAYAPQAYAKPTVNVWALKKDQNIKSLLLRLQGHLGAN
                     TFEIEEDDDLHACSVFLRHTQCAEFRAFIFTFGQKPNRYGIHLEYPDVATSGNLFEAH
                     ENIALKSLVEMLAVHFDIAHIHHLALL"
     gene            complement(799..>2151)
                     /locus_tag="PKZ52_17690"
     CDS             complement(799..>2151)
                     /locus_tag="PKZ52_17690"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNG61660.1"
                     /translation="NRQQQETGMSSIATAKLDLFYLSVVILTGITGSVIAKKIKVPDI
                     VFFLVLGIAIGPAAFNLLNVPADSTLNQLILTLGACYLLFEGGATLRFAVLKEIWLTL
                     LVIATLGVVVTGAITSGFALAFGLPLGIALLLGAVTASTDPATLVPIFKQVPIKDRVA
                     QTVMSESALNDAMGAILTFTVVGFVVGGSEALQPTHIIKELLWEAGGGILIGAVLGYL
                     TALLMAHAALGIFRNHLPFLIVLAVIITFLVTDQVHASGFMAVFTCGVIVGNKEFFRL
                     PLDHHEHEYLEDYVGNTALLMRMFIFILLGSQVDFSLLGQYLWVGVALLLAFMFIARP
                     LTVLLCAAPDRRAKWSWQELVFMSWTRETGVIPAALIGILSGMQVPGVEVAGAITFIF
                     ILGTILIQAPTTALLAGKLGLLANQQLISEPGKKPFEREQSAPIDLTSSAQAHSAPIK
                     "
BASE COUNT          558 a          496 c          557 g          540 t
ORIGIN      
        1 cgccaaggcc aaatagggcg atttttttac cggtaaaatc cggcttgcct gcagtggtta
       61 aaaattcttc ccagttggtt tcgatgcagc ctgcgctgcg gccgggaata tcgccaacgc
      121 cataactggg tgtgccgaga attaaaaaat cgtatttgag cagttcatcc acacttatgc
      181 ggttgacatt cagcggtttg tccgccaggt catcgccgag ttttttgtga atctttttgg
      241 caatcaaacg ggtggtgccg gtttcggtgc cgaagaaaat accgattttg ctcatggggc
      301 ggctccttta taaaagtgcc aggtggtgga tatgggctat gtcaaaatgc acagcgagca
      361 tctccaccag ggatttaagg gcaatatttt cgtgggcttc aaataaattg ccgctggtgg
      421 caacgtctgg atattccagg tggataccgt agcggttggg tttttgccca aaggtaaaaa
      481 taaaggcgcg aaattcagca cactgggtat ggcgtaaaaa cacgctgcaa gcgtgcagat
      541 cgtcgtcttc ttcaatttca aatgtattgg ctcccaggtg cccctgcaag cgcaacaata
      601 ggcttttaat gttttgatct ttttttaagg cccaaacgtt aaccgtaggc tttgcatagg
      661 cctgcggggc atatgcctgt tgattgctcg tgttcatggt catcctccta gggattaaga
      721 tgaataacgc caaaccttgc ccaatgaatg cgcccagaca cctttaaaac ttaccttccc
      781 gctgtttctg cggttgcctt attttatcgg cgcggagtgt gcctgggcgc tgctggttaa
      841 atctataggt gcactttgct cgcgctcaaa tggttttttg cctggttctg atattaattg
      901 ctggttcgct aaaaggccca atttgcccgc caggagtgca gtggtagggg cctgaatgag
      961 aattgtgccg agaataaaaa taaaggtaat agcgccagcg acttcaacgc cgggcacttg
     1021 cataccgctt aatatgccga tgagcgctgc aggtattacg ccggtttccc gcgtccaact
     1081 cataaatacc agttcctgcc aactccattt cgcgcggcga tcaggggctg cgcacagcaa
     1141 cactgttaag gggcgggcta taaacataaa ggcgagcagg agtgccaccc ctacccaaag
     1201 gtattgtccc agcaggctaa aatccacttg agaacccagc agaataaaaa taaacatacg
     1261 cattaacagc gctgtattgc ccacatagtc ttctaaatat tcgtgttcgt ggtgatctag
     1321 gggcaggcgg aaaaactctt tattgccgac tataacgccg caggtaaata ccgccataaa
     1381 gcccgaggca tgcacttgat cagtgaccag aaaggtaatg atgaccgcta atacaattaa
     1441 aaagggtaag tggttgcgaa aaattcccag ggcggcatgg gccattaaga gtgcggttaa
     1501 ataaccgaga acggcgccaa ttaaaatacc gccgccggct tcccagagta attctttaat
     1561 gatgtgggtt ggttgcagtg cttcgctgcc acccacaaca aaacccacta ccgtaaaggt
     1621 gagaatggca cccatggcgt cgtttaaggc ggattcgctc attacggttt gcgccacgcg
     1681 atctttaatg ggcacctgtt taaatatggg tactagggtg gcagggtcgg tggaggcagt
     1741 gactgcacct aataataagg cgatacccag gggtaaacca aaggccaatg caaaaccgct
     1801 ggtaattgcc ccggttacca ctacacccag ggtggcgatc accaataggg tgagccatat
     1861 ttcttttaac acggcaaagc gcaaggtggc gccgccttca aataataaat aacaggcgcc
     1921 gagggtgagt attaattgat tgagggttga atcggcgggc acgttcagta ggttaaaagc
     1981 ggcagggccg atggcgatgc ccagaacgag aaaaaatact atgtcaggca ctttgatttt
     2041 tttggctata acacttcctg tgatgccggt tagaattacg acacttaaat aaaataaatc
     2101 tagttttgcg gtggcgatgc tcgacatgcc ggtttcctgt tgctggcggt t
//
LOCUS       DAOQGS010000682         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34347_length_2150_cov_3.267780, whole genome shotgun sequence.
ACCESSION   DAOQGS010000682 DAOQGS010000000
VERSION     DAOQGS010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34347_length_2150_cov_3.267780"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKZ52_17695"
     CDS             complement(<1..516)
                     /locus_tag="PKZ52_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNG61661.1"
                     /translation="MIQYSTLMAQGLWKNNPALVQLLGLCPLLAVSNNVVNALGMGFA
                     TIWVLMGSNIAIAAIARFVPAAIRLPCFVMVIAAFTTSAELLLQAFSYPLYAILGIFI
                     PLIVTNCVILARAESFASKAPISAAAVDGLAMGAGFTLVIVLLGALRELLGTGGVCAD
                     MQLLFGEAARAM"
     gene            complement(513..1169)
                     /gene="rsxG"
                     /locus_tag="PKZ52_17700"
     CDS             complement(513..1169)
                     /gene="rsxG"
                     /locus_tag="PKZ52_17700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNG61662.1"
                     /translation="MHSPLIRALRTNSLSLTAFASCCALLLGGIYQWTQAPIAQQQLR
                     IQQAALDEIIPAAEHDNAWDKDFFTLNDEDWQALGAPNNRKVFVARQGDAIMGYILPI
                     KTSQGYSGDIELLLGVYVDGRIAATRVVAHKETPGLGDKIDIKRHTWITGFNLKSLNE
                     PKEELWRVKKDNGVFDQFAGATITPRACVAAIKQGLRVFDTRKQQWQQAFTAKRVENP
                     "
     gene            complement(1174..>2150)
                     /locus_tag="PKZ52_17705"
     CDS             complement(1174..>2150)
                     /locus_tag="PKZ52_17705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNG61663.1"
                     /translation="LAVQLWLYGPGNLANACVLMLLCAGFEAGFLALREKPIKPALLD
                     GSALVTGALLAVSLPPYCPFWLLVSAAFFAMVLAKHLYGGLGFNPFNPAMVAYVILLV
                     SFPLPMTQWPMSESLLPASASKPDVYTVLQAWFGSGWDITGPDAISGATSLDLLQHKQ
                     GNTLAAWLAQQRVFNQAVFAASGSEWINLGFLCGGLLLLFKRVFTWHAPVAFLTSLGV
                     LAAFFYDAGSSQSPGSPLLHWFSGGTMLGAFFIITDPVTSAVSPKGRLIFGALIGGLV
                     YVIRAWGNYPDAIAFAVLFGNFAAPLIDFFTVPKTYGHNKSPSAKRFF"
BASE COUNT          558 a          582 c          573 g          437 t
ORIGIN      
        1 catggcgcgg gcggcttcgc caaataataa ctgcatatcg gcacacacac cgcctgtgcc
       61 caacaattcc cgcagggcgc caagcaagac aattaccagg gtaaaaccgg cccccatggc
      121 caggccgtcc actgcggcgg cgctaatagg tgctttgctg gcaaaacttt ctgcccgcgc
      181 gaggatgaca caattggtaa caatcagcgg aataaaaata cctaaaatcg catacagcgg
      241 gtaactaaag gcttgtaata ataattctgc gctggtggta aaggcggcaa ttaccatcac
      301 aaaacagggt aggcgtatgg cggcgggcac aaagcgcgct atggcggcaa tggctatatt
      361 cgagcccatt aatacccaaa tagtggcaaa acccatccct aaagcattta ccacattatt
      421 gctgaccgca agcagagggc agaggccgag cagttgaacc agtgcggggt tatttttcca
      481 cagcccctgt gccattaagg ttgagtattg gatcatggat tttccacccg tttagcggta
      541 aacgcctgtt gccattgctg tttgcgcgta tcaaataccc ttaagccctg tttgatggcg
      601 gccacacagg cgcgcggtgt aatggtggca ccggcaaact gatcgaacac accattatct
      661 tttttcacac gccacagctc ttccttgggt tcattgaggc tttttaaatt aaagcctgta
      721 atccaggtgt ggcgtttaat atcaatttta tcgcccagtc cgggagtttc tttatgggca
      781 accacccgcg tggcggcgat gcggccatcc acataaaccc ccagtaataa ttcgatatcg
      841 ccggaataac cctggctggt tttaatgggc agaatatagc ccataattgc atccccctgt
      901 ctggcgacaa acaccttgcg attgttggga gcacccaggg cctgccaatc ctcatcatta
      961 agcgtaaaaa aatccttatc ccaggcattg tcgtgttcgg cggcgggaat aatttcatcc
     1021 aaagccgcct gttggatgcg caattgttgc tgcgcgatgg gcgcttgagt ccattggtaa
     1081 atgccgccta ataataaggc gcagcagctg gcaaatgccg tcaggcttaa actattggtg
     1141 cgcagagcgc ggatgagggg gctgtgcatg tgcttaaaaa aaccgcttag ccgagggcga
     1201 tttattgtgc ccataggttt tgggtacggt aaaaaaatca attaagggtg cggcgaaatt
     1261 gccaaataac accgcaaagg cgatggcatc cgggtaattg ccccaggcgc gaatcacata
     1321 caccaagccg ccaattaagg cgccaaatat caaccggccc ttggggctga cggcgctggt
     1381 gacggggtcg gtgataataa aaaacgctcc caacatggtg ccgccactaa accaatgcag
     1441 gaggggcgag cctggacttt gggagctgcc cgcatcgtaa aaaaaggcgg cgagtacacc
     1501 taagctggtt aaaaacgcca ccggtgcgtg ccaggtaaac acccgtttaa acagtagaag
     1561 caggccgccg cataaaaaac ccaggtttat ccattcgctg ccgctggcgg caaacactgc
     1621 ctggttgaat acccgctgct gggcaagcca ggccgcaagc gtgttgccct gtttgtgttg
     1681 tagcaaatct aaactggtgg caccgctgat ggcatctggg ccggttatgt cccatccgct
     1741 gccaaaccaa gcctgcaaca ctgtgtaaac atcaggctta ctggctgagg cgggcagcag
     1801 gctctcgctc atgggccact gggtcatggg cagcgggaag gacaccagca aaataacata
     1861 ggccaccatg gccggattaa aggggttaaa acccaagccg ccgtagagat gcttcgccag
     1921 caccatggca aaaaacgccg cgctcaccaa cagccaaaac gggcagtagg gtggcaggct
     1981 gaccgccagg agggcgccgg tgaccagcgc gctgccgtct aaaagggctg gttttatggg
     2041 cttttcccgc agggctaaaa acccggcctc aaagcctgca cacagcagca tcaagacgca
     2101 ggcattggcc aaattgcccg gcccatacag ccacaactgc accgcaagtc
//
LOCUS       DAOQGS010000683         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34477_length_2145_cov_3.465550, whole genome shotgun sequence.
ACCESSION   DAOQGS010000683 DAOQGS010000000
VERSION     DAOQGS010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34477_length_2145_cov_3.465550"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKZ52_17710"
     CDS             complement(<1..755)
                     /locus_tag="PKZ52_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61664.1"
                     /translation="MGIELEEVDENNNLIKNLGELTNANGPGSLYRFDMTFNGGQPGY
                     RYFRLRANADGASKTSDKTVLWVSDLDVRKPFVNEADIKGTDVNLDSLKSYNLVIILN
                     PNSKIPPTREILDKALESVNGKLIQCIPQLNQCLAKVDNLNIEEVVKNLQFKSDGFIK
                     DAYLDPIEIVPTSGESSIETKSGEQSRWHLDRIAHEEAWQLMDQTQHWLGVGVIDNGV
                     RCARINDIDDASGPNSGPNSCINSFSILEKSNHA"
     gene            787..1071
                     /locus_tag="PKZ52_17715"
     CDS             787..1071
                     /locus_tag="PKZ52_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61665.1"
                     /translation="MKNATLVSPTVLIDPSEVEGAGRSIASVLEPELLLLLLPLLLLL
                     LLLLLLLLDETGSLGALLPPPLPQPAAVMKIKDETSWIHLINRFDMRMLR"
BASE COUNT          549 a          467 c          462 g          667 t
ORIGIN      
        1 gcatgattgc ttttttcaag aatagaaaag gagttgatgc aactattggg gccactgttg
       61 gggccacttg catcgtcaat atcatttatt cttgcacagc gcacgccatt atcaatcacg
      121 cccaccccga gccaatgttg tgtttgatcc attagttgcc aggcctcctc atgagcaatc
      181 ctatccaggt gccagcggct ctgttcgcct gatttagttt ctatgctaga ttctccactg
      241 gtcggtacta tctcaatcgg atcaagatat gcatctttaa tgaacccatc actcttaaac
      301 tgtaaatttt tgacaacttc ctcaatgttc agattatcga ctttcgccaa acattgattt
      361 aattggggaa tgcattgaat taatttgccg ttaacagatt cgagtgcctt atccagtatc
      421 tctcttgtcg gtgggatttt tgagttgggg tttagaatga ttacaaggtt gtagctcttt
      481 agtgaatcta agttaacatc agttccttta atatccgctt cattcacaaa cggcttacga
      541 acatccaaat cagacaccca aagaacagtc ttatcagacg ttttgctagc cccatcagcg
      601 ttggctctta gtcgaaagta cctgtagcct ggttgcccac cgttaaacgt catatcaaac
      661 ctataaagcg aaccgggacc attcgcgttg gtgagttctc ctaggttttt tatgagattg
      721 ttattctcat cgacctcttc taattcaatg cccacgaggc ttttctccga cggtgtggtg
      781 acgcgcgtga agaatgccac tttagtttca ccgaccgtat taattgatcc ttccgaagta
      841 gagggggcag gcagatcaat tgcaagtgtg ttggaacctg agctactact actgctactg
      901 ccactgctac tgctactgct actgctactg ctactgctgt tagacgagac aggctctttg
      961 ggcgcgttgc tgccgccacc gctaccgcaa cctgccgctg ttatgaagat aaaagatgaa
     1021 actagttgga tacacctaat taatcgcttc gacatgcgaa tgctccgttg atagttgttt
     1081 aatgtatttt ctacatcata gattcaagag gagactgaat aatcttctct cttatgcatg
     1141 agctgctttc agctcgtaca ctattattaa cgctagcgct taatcccatc ccatttatag
     1201 atgtagatca attcaagatg gcaagttgaa ttcgtatgag cgcgcaaaat ttagaacaca
     1261 ttattaaaag cgcagtttca taccttgccc acctcaacgg gcgcattagc agattgtgtg
     1321 ccacagaaac tcttccagat aattttgact ttattctgtt caacgcgatc aataattcac
     1381 ctgttttcat cgcttctctt gtcaaacgtt tttaaattgg tttttttatc gccgtattgt
     1441 tagcgtgtta tttggaaggc tctttttgtc ggcgtgacta ttgtaatctc gctcgtcagt
     1501 ttgaaaaaat atcgtcgaaa gatgattgga atttgtgcaa acatgcgttt aaatgagaat
     1561 cattaatttg gcagagtgcg tgtaagatta atcgacaatc ggtttttgct ggcttaatct
     1621 aatttttttg cctcatatgc atttaatgct gctggcaaaa aaaccgacat ttagggagtg
     1681 ctaatttagc ttggttttat aaggggctgc tgccgccaat ttaaaaagta gtatttaaat
     1741 ggcctttggc gttcatgatg ttaagccgat cacagcggct gaagcagcgg ctaaattacc
     1801 tgagcaacaa aacccgcaaa gacaaatata atggcagatg aaactcctgc agccggagag
     1861 gcctttgagt ttgttttcgg ccttttgctg caggagtacg cgtctcacac caggtggctg
     1921 gcaatgttat taagtcaaat ttttaatgcg aatatttatc cctgggttgg aggagcgcaa
     1981 ggtcacgtgg gtgtgacatt gtagagaaat gcaggagcaa ttattgagcg acttgcgata
     2041 tccattcacc agtacaaaaa tgggtgcggc gggagtttgg cggggttcat gtcggaagtc
     2101 gctgcgctct tcctacccac gccattttgg gctttccctc aaatt
//
LOCUS       DAOQGS010000684         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34659_length_2138_cov_5.435910, whole genome shotgun sequence.
ACCESSION   DAOQGS010000684 DAOQGS010000000
VERSION     DAOQGS010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34659_length_2138_cov_5.435910"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1115)
                     /locus_tag="PKZ52_17720"
     CDS             complement(135..1115)
                     /locus_tag="PKZ52_17720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNG61666.1"
                     /translation="MSTPTRLPALDCLRAFAIIIVLLYHYKVVVSSEDTFGYITQIGW
                     MGVDLFFVLSGYLIGDQILGPLARQEPFSIGRFYLRRLLRTLPNYYAVCLLYIFGPDI
                     LIGNHTSDWWRFLTFTQNFDFRPGVTFSHSWSLCIEEQFYLLAPLVFVLTARSGQVVL
                     GSWLLIVFGLGLGLGSRLDAWYSHGLNNMSVNNYYHSIYYSSLSRFDELLPGIAIALI
                     KNAHPILFQRLLARGHYWLGIGTLSFASLAYAYPNLHYKEARGYNFFLSTFGYTALAL
                     SFMCIVLSALAPKSLLGRWVIPGAGSLAVCSYALYLIHKPLFKLLIDPLE"
     gene            1697..>2138
                     /locus_tag="PKZ52_17725"
     CDS             1697..>2138
                     /locus_tag="PKZ52_17725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNG61667.1"
                     /translation="MGNVTFEQLEQEAPERRDLYQQFAGQEEEERISFHYENDPEFFK
                     IVTGDSWWTYSSPLWEDTDLTITQAQETKFAWYAQQMNLSPGKTILDVGCGWGGPLVH
                     FCKQYQVSGVGISVSPRGLQVARVRAAEHGVDAQFLLSHWQHLPD"
BASE COUNT          625 a          464 c          536 g          513 t
ORIGIN      
        1 ggaaggtcat tttgccatct ggcgcggggg ttttacacaa ttctacgaag agccacaaat
       61 aataaccagg ccgccaatag gctgatggcc ataataatcc cggcgcctaa taaatcatgg
      121 ctgtgaatgc ccgcttattc aagtgggtca attaataatt taaatagtgg tttgtgaatt
      181 aaatacagcg cgtaggagca aaccgctaaa ctgccagccc caggaataac ccagcgcccc
      241 aacaggctct taggtgccaa tgcggacaac acaatacaca taaaacttaa ggcaagagcg
      301 gtgtagccaa acgtacttaa gaaaaagtta taacctctgg cttctttgta gtgcagattg
      361 gggtaggcgt aagcgaggct ggcaaagcta agcgtgccaa tacccagcca ataatggccg
      421 cgtgccaata aacgctgaaa taaaataggg tgggcatttt taattaaggc aatagctatg
      481 cccggcaata attcatcaaa acggctaagg cttgaataat aaatcgagtg gtaataatta
      541 ttgacactca tattattgag cccatggcta taccaagcat ccaagcggct gcccaaacca
      601 agccctaaac caaacacaat tagcagccaa ctgcctaaaa ccacttgccc gctgcgggct
      661 gtcagtacaa atactagtgg tgccagtaaa taaaattgct cctctataca gagcgaccaa
      721 gagtggctaa aggtgacgcc tgggcgaaag tcaaaattct gcgtgaaggt tagaaatcgc
      781 caccagtctg aagtgtggtt gccgattaaa atatccgggc caaaaatgta cagcaaacag
      841 accgcataat aattgggcag cgtgcgcagc aggcgacgca gataaaacct accgatagaa
      901 aacggctctt gccgcgccaa cgggcctaaa atctgatcgc caattaaata gccgcttaag
      961 acaaaaaata aatccacacc catccagcca atttgggtga tatacccaaa agtgtcttca
     1021 ctggaaacaa ccaccttata gtggtacagc agcacaataa tgattgcgaa cgcacgcagg
     1081 caatcgagtg cgggtaggcg ggtaggggta gacatggcta aatcaatagg agggcatttg
     1141 ttgagccgcg cagggtaaag gattacatca taactgtcta ttttgctggc tgggcggacg
     1201 tcatgaataa atcgtattgc tgccccaccg gtgatacatc ctgtatagat gcgtcaaatt
     1261 ttgacagatc tacgatctgt atctatttgc cgctgatgat gtaatgcgat gacgatggcc
     1321 aaagtttggg ctgctaaaat cgcagggtgt gttttatatg gcttcaacac ccagcctaga
     1381 gtaaaaaatg catgtgcggg cagggcaccc gaattccctg gcctcacttg cagattataa
     1441 atgcagctag attatattca gtgccgctca tgtacagggc tgtaaacggg gaatatcggg
     1501 tggctagtga cgccaacttt ttagctgcca aagcgcatgt ggttttctaa aaggcgggta
     1561 atggctgttt gctgcatatt ctttgctatt gcgcttggtg agttgctaat gcaaaatcgc
     1621 ggcagggtta agcgcgttat aaattacctt ttgtgttaat taattggctg ataaaatatt
     1681 gagttgggag gcgtatatgg gtaatgtcac atttgagcaa ttggaacaag aggcccccga
     1741 gcgtcgggat ttataccagc agtttgcagg ccaggaggaa gaggagcgga tttcctttca
     1801 ttacgaaaac gatcctgaat tctttaaaat agtcacaggc gatagctggt ggacttattc
     1861 aagcccgctg tgggaggaca cagatttaac cataacacag gcccaggaaa ctaaatttgc
     1921 ctggtacgca caacagatga acttatctcc gggtaaaaca attctcgatg taggctgtgg
     1981 gtggggtggc cctttggtgc atttttgcaa gcaataccaa gtgtcgggtg ttggtatttc
     2041 ggtttcaccg cgtggtctgc aggtggcgcg ggtgcgagcc gctgagcatg gggtagatgc
     2101 acaatttctt ttatcgcact ggcaacattt gcccgata
//
LOCUS       DAOQGS010000685         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34660_length_2138_cov_5.220355, whole genome shotgun sequence.
ACCESSION   DAOQGS010000685 DAOQGS010000000
VERSION     DAOQGS010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34660_length_2138_cov_5.220355"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..816
                     /locus_tag="PKZ52_17730"
     CDS             379..816
                     /locus_tag="PKZ52_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNG61668.1"
                     /translation="MISVQTEDFDAGKEAALLRRHLGIGAVVSFIGLVREFAVNPDAC
                     AGLYIEHYPGMTEKSLQGLATQAQQRWSLSGVRVIHRVGQLAPGEQIVLVITAAAHRK
                     DAFAACEWLMDMLKTQAPFWKREGDDWVAAKDSDQAALKRWGF"
     gene            964..1158
                     /locus_tag="PKZ52_17735"
     CDS             964..1158
                     /locus_tag="PKZ52_17735"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNG61669.1"
                     /translation="MATNLSIDTNLLNEALLISGLATKKATVNQALMEFIQRRKQREI
                     TTLFGQLPQDNNYDYKKGRE"
     gene            1155..1562
                     /locus_tag="PKZ52_17740"
     CDS             1155..1562
                     /locus_tag="PKZ52_17740"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNG61670.1"
                     /translation="MKGILVDTCVWSIALRGGKAKDAQITEALTQLIDDNQAKIIGVI
                     RQELLSGYSDKQSYERLKAKLKYFPNEPILDSDYETAAEYANFCRTKGIQGSHTDYLI
                     CAVAIRARLKIFTTDKDFSNYSKHLPIAIYQLK"
     gene            complement(1570..>2138)
                     /locus_tag="PKZ52_17745"
     CDS             complement(1570..>2138)
                     /locus_tag="PKZ52_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61671.1"
                     /translation="KVIEVNVIDVGRWGFGSPSTGAKPFAPFSDWMTQRTGTVEAEVN
                     FNKGKGGWRPALDSVHMSLSDYLWITKENLIFLFFADSWPDMRAGRLIDDLSSIMRWL
                     WAPLFIFAIIVLIKDAKKLRRQWLLTAVIAAWFVVQGLIPIAINEGRYRKPLEGLLVA
                     QIVLWLAARQGAIRLPVAERRDDNPAKA"
BASE COUNT          560 a          583 c          547 g          448 t
ORIGIN      
        1 ccactggagg gctggcaggc tcgcctgccg gggtggttgc tctgacaggg ctcgggtatt
       61 gccagggggt gaggattttt taccggcaaa ggtggccgct tgccaacaaa acacagcggc
      121 ccccaccacc ccaccccttc ggggcacccc tccaatggag gggaatctca ggccgtgccg
      181 tagcgaccac tatcggccaa tccttcatgt agtgacatta ctttcaaagg aggggaattt
      241 taggcactgc cctgatcata caatccttag gctaatgatt acccaggcgg gtttttaggt
      301 taccgctgcc gggcaaactg gcatcatagg cacctgctgt tggcagtttg aaataggttc
      361 atatcccaaa ggctccctat gattagcgtg caaaccgaag actttgatgc gggtaaagaa
      421 gcggccctgc tgcgccgcca cctgggcata ggcgctgtgg tgagttttat cggcctggtg
      481 cgggagtttg ccgtcaaccc cgatgcatgc gcgggccttt atatagaaca ctaccccggc
      541 atgacggaaa aatccctcca ggggctggca acccaggccc agcagcgctg gagtttgagc
      601 ggggtgcggg tcattcaccg ggtggggcaa ctcgcccccg gcgagcagat tgtattggtg
      661 atcaccgccg ccgcccaccg caaggatgcc ttcgccgcct gcgaatggct aatggatatg
      721 ctcaaaaccc aggcgccctt ttggaagcgg gagggcgatg actgggtggc ggccaaagac
      781 tccgaccagg ccgccctaaa gcgctggggc ttctaagcct tagggatcgg gacaccaaca
      841 caagtcgcta tctgcgacta acctgcccag ccacacagca acagcacgca aatggagtgt
      901 tagtctgtgt gctactatgt gtagcatcat gtgtagaact gcgtgtaaac agaggaattt
      961 atcatggcga caaacctttc aatcgacacg aacttgctta acgaagcgct gttaatcagc
     1021 ggcctagcaa caaaaaaagc taccgtaaat caagcgctta tggagtttat ccaaagacgc
     1081 aaacagcgcg agattaccac tttattcggc caactgcccc aagacaacaa ctatgactat
     1141 aaaaagggac gtgaatgaaa gggatactcg tcgatacatg cgtatggtca atcgccctta
     1201 ggggcggcaa ggcaaaggat gcgcaaatta ctgaagcact gactcaactt atagatgaca
     1261 accaagcaaa aataattggc gtcattaggc aagagttgct ctccggatat agcgacaaac
     1321 agagttacga acgattaaag gcaaagctga aatactttcc caatgagcct attttggatt
     1381 cggattatga aactgcagca gaatacgcca acttttgtcg caccaagggt atacaaggct
     1441 ctcataccga ttatttaatt tgtgccgtag ccataagggc aaggctaaag atatttacga
     1501 ctgacaagga cttcagcaac tactctaagc acctaccaat agccatctac caattaaagt
     1561 aactcacatt taagccttcg ccgggttatc gtccctacgc tctgccacag gcaaccttat
     1621 agccccctgg cgcgccgcta accacagaac tatctgcgca accaacaagc cctccaaggg
     1681 tttgcggtaa cggccttcgt tgatggcgat ggggattaag ccctgcacca caaaccatgc
     1741 ggcaattacg gcggttaaca gccactggcg gcgcagtttt ttggcgtctt ttatcagcac
     1801 aataatggcg aagataaaca gcggggccca cagccagcgc atgattgagg agagatcatc
     1861 aatcaatcgc ccggcgcgca tatccggcca agaatccgca aagaacagaa aaattaggtt
     1921 ttctttagta atccaaaggt agtccgacag gctcatatgc acgctatcca acgcgggccg
     1981 ccagccgcct ttgcccttat taaaattcac ttccgcttcc accgtgccgg tgcgctgggt
     2041 catccaatca ctaaagggcg cgaagggttt agcgccggta gagggcgagc caaaccccca
     2101 gcggcccacg tcgatcacat tcacttcaat gacttttt
//
LOCUS       DAOQGS010000686         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34662_length_2138_cov_5.085934, whole genome shotgun sequence.
ACCESSION   DAOQGS010000686 DAOQGS010000000
VERSION     DAOQGS010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34662_length_2138_cov_5.085934"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKZ52_17750"
     CDS             complement(<1..639)
                     /locus_tag="PKZ52_17750"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNG61672.1"
                     /translation="MYSLNLRRLNAADADFSQQLDQLLQFEASHEESVQGRVKTILAA
                     VKSRGDEALLEYTNLFDRRTLTAISEVIIDRAQLQAALARIPADQAEALQQAAERVRD
                     YHSRQQQPSWSYQDDQGNLLGQKVTPLDRVGLYVPGGKAAYPSSVLMSAIPAKVAGVP
                     EVVMVSPSPDGQVNDLVLAAAAVSGVERIYSIGGAQAVAALAYGTATIPKVDK"
     gene            complement(679..1317)
                     /gene="hisG"
                     /locus_tag="PKZ52_17755"
     CDS             complement(679..1317)
                     /gene="hisG"
                     /locus_tag="PKZ52_17755"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNG61673.1"
                     /translation="MSQLTIALTKGRILQETLPLLAAAGIEPLEDISKSRKLTFDTTS
                     PEVRLLILRGSDVPTYVEFGAADVGVSGKDTLLEHIGNSFYELLDLKIARCTLMTAAL
                     AGAPSKSGRIRVATKYVNVAKRYYAAQGRQVDIIKLYGAMELAPVLSLADEIVDIVDT
                     GNTLRANGLEPRETIAQISSRLIVNKASMKLKHHQIEALIDAVESAVGELSA"
     gene            complement(1327..>2138)
                     /locus_tag="PKZ52_17760"
     CDS             complement(1327..>2138)
                     /locus_tag="PKZ52_17760"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNG61674.1"
                     /translation="GLKGAHIFMDTVTVGGTENLLMAATLAKGKTVLENAAREPEIVD
                     LAECLVSMGAQIEGIGTDHITVHGVPSLKGCTHRVMPDRIETGTYLTAAAATGGKVML
                     RDTRADILEAVLLKLEEAGAEITCGPDWIALDMHGRRPKAVNLRTAPYPAFPTDMQSQ
                     FTALNAVAEGVSRVTETIFENRLIQVHELNRMGANISLEGNTAIITGVAKLKGAPVMA
                     SDLRASASLVIAGMAAEGETLVDRIYHIDRGYECIEEKLQALGVDIRRVPG"
BASE COUNT          412 a          615 c          649 g          462 t
ORIGIN      
        1 tttgtccact ttggggatgg tcgcagtgcc gtaggccagg gccgctacag cctgggcgcc
       61 gccgatgcta tagatgcgct ccactccgga taccgcagcc gccgccaata ccaaatcgtt
      121 cacctgcccg tcgggggacg ggctcaccat aaccacttcg ggtacgccgg cgactttggc
      181 tggaatagcg gacatcaaga cggaggacgg ataggccgcc ttgccgccgg gcacatagag
      241 gccaacgcga tccagggggg tgactttttg accgagcaga ttgccctggt cgtcttggta
      301 gctccaggag ggttgctgtt gacggctgtg gtagtcgcgc acgcgctcgg cggcttgttg
      361 cagggcttcg gcctgatcgg caggaatacg ggcgagggca gcctgtaatt gcgccctatc
      421 gataatgact tcgcttattg ccgtcaaagt gcggcggtca aacagatttg tgtactccag
      481 taacgcctca tcgccacggc ttttaacggc ggccaaaata gttttgacgc gtccctgcac
      541 ggactcttcg tggctggctt caaactgcaa caattggtcg agctgctggg aaaagtcggc
      601 gtccgccgcg ttaaggcggc gcaaattcaa ggaatacatg gggcacctgt tgctaagtgg
      661 gcggtaaggc ttcagctcct aggctgacag ctctcctacg gcagattcta ccgcatcgat
      721 taaggcttcg atttgatggt gcttgagttt catggaggcc ttgttaacga tcagccggga
      781 gctaatttgc gcgatagttt cgcgaggctc aaggccgttg gcgcgcaatg tattgccggt
      841 atccactata tcgacgatct catcggcaag gcttaacacc ggggccagct ccatggcgcc
      901 gtagagtttg atgatgtcca cctggcgccc ctgagcggcg tagtaacgct tggccacatt
      961 gacgtattta gtggccacgc gaatgcggcc gcttttgctc ggcgccccgg ccaaggcggc
     1021 cgtcatcagg gtacaacggg cgattttcag atccagcaac tcatagaagc tattgcctat
     1081 gtgctcaagc aaggtgtcct tgccagacac tcccacatcg gccgcgccaa attccacata
     1141 ggtgggcaca tcactgccgc gcagaattag caggcgcact tcgggagagg tagtgtcgaa
     1201 ggtgagtttg cggcttttgc tgatgtcttc caacggctcg atgcccgccg ccgccagtaa
     1261 gggcagagtt tcctgcagga tacggccctt ggtgagggcg atggtcagtt gcgacataga
     1321 cacctcttaa ccgggcacgc ggcgaatatc cacgcccagg gcctgtagtt tttcttcaat
     1381 acactcatag ccgcgatcga tatggtaaat gcgatccaca agggtctccc cttcggcggc
     1441 cataccggcg atcaccaagc ttgctgaggc ccgcaggtcg ctggccataa ccggggcgcc
     1501 cttgagttta gccaccccgg tgataatggc ggtattgccc tccaggctga tattagcgcc
     1561 catgcggttg agctcgtgca cttggatcag gcggttttca aagatggttt ccgtcacccg
     1621 ggagacacct tcagccaccg cattcagggc ggtgaactgg gactgcatat cggtgggaaa
     1681 agccgggtaa ggcgcggtgc gcaggttgac ggctttgggg cggcggccgt gcatatccag
     1741 agcaatccaa tccgggccgc aggtgatctc ggcgcccgct tcctcaagct taagcagaac
     1801 tgcctcgaga atatccgccc gggtatcccg cagcatgacc ttaccgccgg tggcggcggc
     1861 agctgttaaa taagtgcctg tctcgatgcg atcgggcatc acccggtggg tgcagccctt
     1921 caggctcggc acgccgtgca cggtaatgtg gtcggtgcct atgccttcga tctgggcacc
     1981 catgctcacc aggcattccg ccaggtcgac aatttccggc tcccgggcag cgttttctag
     2041 aacggtttta cccttggcca gggtggcggc cataagcagg ttttcggtgc cgcccacggt
     2101 gacggtatcc ataaagatat gggcgccctt aaggccct
//
LOCUS       DAOQGS010000687         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34719_length_2136_cov_4.214801, whole genome shotgun sequence.
ACCESSION   DAOQGS010000687 DAOQGS010000000
VERSION     DAOQGS010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34719_length_2136_cov_4.214801"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1762)
                     /gene="pilB"
                     /locus_tag="PKZ52_17765"
     CDS             complement(38..1762)
                     /gene="pilB"
                     /locus_tag="PKZ52_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNG61675.1"
                     /translation="MSISPPLSGLARRLVRDGVINEEQAQQAILAATKDKVSLPYALV
                     SSGQVNALYILHSASDEFGTPIVDTDSLIGFNFDRSLVDSKLIRKHQALPIFRRGNRL
                     FIAIADPTNLHALDEIKFHTGLNTESILVAADKLQKKIDTYLQQEEEPLGSGLSEIDT
                     DGLDSLDIEAVTEDAGEGPDTSDIDEAPVVRFINKVLLDAIRSGSSDIHFEPYEKIYR
                     VRFRTDGILREVARPPVNLTTRIAARLKVMSQMDLSERRIPQDGRIKMKISKTRAIDF
                     RVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEDNQKKMYLDALEQPQGMILVTGPTG
                     SGKTVSLYTGLNILNTIERNISTAEDPVEINLEGINQVNVNLKVGLGFPEALRSFLRQ
                     DPDVIMVGEIRDLETASIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPTFNVATT
                     VSLIIAQRLARRLCPHCKKPATDIPKEVLIEEGFEDIGIPMGEIKLFEPVGCEKCTDG
                     YKGRVGVYEVVRITPEISRIIMAGGNSIEIADAAKRAGFNNLRISALRKTAIGLTSLA
                     EANRVTKD"
     gene            1989..>2136
                     /locus_tag="PKZ52_17770"
     CDS             1989..>2136
                     /locus_tag="PKZ52_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNG61676.1"
                     /translation="MRNQQGFTLIELMIVIAIIGILAAVALPAYQDYTVRSKMSEAVL
                     QSDAL"
BASE COUNT          534 a          486 c          496 g          620 t
ORIGIN      
        1 atagtattgg ctgcggagga aggtattgcc ataggaatta atccttcgtt acacggttgg
       61 cctcggcgag gctagttaaa ccaatggccg ttttacgtag ggccgaaata cgtaaattat
      121 tgaagcctgc tcgtttagca gcgtcagcaa tttcgatgga attgccaccc gccattataa
      181 ttcgggaaat ttcaggtgtg atgcgaacca cctcataaac ccccacccgc cccttgtagc
      241 catctgtaca tttctcacag ccaactggtt caaacagctt aatttctccc atgggtatgc
      301 caatatcttc aaagccttct tcaattaaaa cttccttggg tatgtcggtg gcgggctttt
      361 tacagtgggg gcaaagcctg cgggcaagcc gttgagcaat aataaggctt actgtagtcg
      421 ctacgttaaa tgtgggtaca cccatattaa gcaaacgagt taaggtttcg gctgccgaat
      481 tcgtatgaag ggtagataga accaagtggc cagtttgagc cgctttaatc gcgatagaag
      541 cggtttccaa gtcgcgaatt tcccccacca tgattacatc cgggtcctgc cgcaagaacg
      601 agcgtaatgc ttcgggaaag cccagtccga cttttaaatt aacattaacc tggttaatgc
      661 cttccaaatt aatttctaca gggtcttctg cggtggaaat attgcgttct atggtgttta
      721 ggatatttaa ccccgtgtag agtgagactg ttttgccaga gccggtaggt ccagttacca
      781 gaatcatccc ctgaggctgt tctagcgcat ccaaatacat ttttttctga ttatcctcat
      841 agcctaacgc gtcaataccg agcttggcgc tggaagggtc taagatacgt aatacaattt
      901 tttcgcccca aagcgtaggt agggtattga cgcgaaaatc gatggcgcga gtcttggata
      961 tcttcatttt gatacgccca tcctggggta tgcgtctctc agagagatcc atctgggaca
     1021 ttactttcaa ccgggcggca atgcgggttg ttaagtttac gggcggtcta gccacctctc
     1081 gcagaatgcc atccgttcgg aagcgcacgc ggtagatttt ttcatagggt tcaaagtgaa
     1141 tatcggagga tcccgagcga atggcatcca ataatacttt gtttataaaa cgtaccactg
     1201 gggcttcgtc gatatccgat gtgtcaggcc cctcgcctgc gtcctcggta actgcctcta
     1261 tatctaagct gtctaatcca tctgtatcga tttcactaag gccgctgccc agaggctcct
     1321 cttcctgttg caagtaagta tcaatttttt tctgcaactt atcagccgca actagaatgc
     1381 tttcagtgtt taagcctgta tgaaatttaa tctcatccag tgcatgcaaa ttagttgggt
     1441 ccgcaattgc gatgaaaagg cgattgcctc gcctgaaaat tggcagcgct tggtgtttgc
     1501 gaattaattt ggaatcgaca aggcttctat caaagttaaa accgatgagg ctgtcagtat
     1561 ctacgatagg ggttccaaac tcatctgagg cagagtgcaa aatatacaag gcattgacct
     1621 gaccactact caccaatgcg tagggtagtg acaccttgtc tttggtcgct gccaaaattg
     1681 cttgttgggc ctgttcttca ttgataacgc cgtctcttac caggcgtcta gccaaaccac
     1741 ttaggggggg ggagatactc atgccttatt cttatttaac ttcggaccgt caaattctag
     1801 cctctttgtt gacctatagc caaggtggag ttagtggccg taaattgtca tcttctgtca
     1861 aagagtgaaa gtatgatcct atcgaaatgc agtaatgcac tatttctccg cgttggacag
     1921 ggcattttta gtatttggca tatctcttga atgctgtgat ttactaccca acaacgagga
     1981 gttttcccat gagaaatcaa caaggtttta cattaattga attaatgatt gttattgcga
     2041 taattggtat tttggctgcg gtcgccttgc cagcctatca agactatact gtccgctcta
     2101 agatgtcgga ggctgtattg cagtctgatg cactat
//
LOCUS       DAOQGS010000688         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34770_length_2134_cov_5.073593, whole genome shotgun sequence.
ACCESSION   DAOQGS010000688 DAOQGS010000000
VERSION     DAOQGS010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34770_length_2134_cov_5.073593"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            628..1518
                     /locus_tag="PKZ52_17775"
     CDS             628..1518
                     /locus_tag="PKZ52_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61677.1"
                     /translation="MIWFLVAAIIVIYLVIKIKKSARQVSRRPKSKKLLQQGAQSQST
                     VPKQSSRANKPEWEASYFKPPIKKGYQIFSPSLDVAGIEHCKDDAFNFYRSKSETLQL
                     QPEPDNAHDKNAIKVLGIHDTQTFFLGYVPKKSAALLAKTGLVELVYPRLLRIATDGD
                     HDIKINYTLVGPKNDKHKYNNYLNEQPASKEQKDYLTFFSIPISKGLNSGQAEALISE
                     HEKTASDFELDEWNNYQNILEYFKDDLRETDGIKKVSEPLLIEALKQLKQEGNSYERL
                     YEDIDEVVERLIEIKPELAC"
     gene            complement(1654..1863)
                     /locus_tag="PKZ52_17780"
     CDS             complement(1654..1863)
                     /locus_tag="PKZ52_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61678.1"
                     /translation="MNTPDAAVDAAQTPIPDAKLTHIASHYFGIETLATRNRDALDFH
                     DIAVWNLKAALEAAYRAGRHSAKSL"
     gene            complement(1954..>2134)
                     /locus_tag="PKZ52_17785"
     CDS             complement(1954..>2134)
                     /locus_tag="PKZ52_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61679.1"
                     /translation="PERFGIECPLAICAFRVECAVGFRGDTASADGLVYGGIADAAMA
                     GVGADGDADCGSLLW"
BASE COUNT          620 a          521 c          502 g          491 t
ORIGIN      
        1 ttcgagcagc gagtgccgtc agtaccttgt gacgggcacc tccgtgcaac ccttcgggaa
       61 accattgaat ctgcccgtgt gttacgtcga tggcatgggc taggagggtc tgttgggtgg
      121 gtgtgaggga tacggggggc ataagcaact ccagataacg attaagggtc ggttaacacg
      181 cccatgaacg cttcctttcg gtctaaagcc aagctatttt cgatctatta ataaccgctt
      241 tttaaagcgc aaagtagctg aatgcagtgc ataccctctc ggttatacaa ctgctattat
      301 cggactaggc caagcggttg tttgaatgga gcatcacaga taaaacgggc aaacgactcc
      361 gcccgcccaa ggtttaatct ctctaaggta gcctcgggca gttggggcag tacttaaaac
      421 gcaccgatcc aggatttcac ctaaagaata cgggcattcg ggattattaa gtatggtaca
      481 aacctactcg gacttaacca ttacaaaaga taatcatggc ggccattcgg tgagacttaa
      541 caattaagtc tgaatgagtt gggcaggcac agaccgggca atcaattcaa taaatattca
      601 gtgaacaaac tagctaggaa ggtcttcatg atatggtttt tagtggcagc aattatcgtt
      661 atttacttag tgattaaaat caaaaaaagc gctagacaag ttagtcgccg ccccaaatcg
      721 aagaagctcc tccagcaagg cgctcagtct cagtccaccg ttcctaagca atcatcacga
      781 gcgaacaaac ctgaatggga agccagttac tttaagcccc ccataaaaaa gggataccaa
      841 atattttctc cgagcctaga tgtcgcaggt atagaacatt gcaaggatga cgcattcaat
      901 ttttaccgat caaaaagtga gacgttacag ctgcaacccg aacccgacaa cgctcacgat
      961 aaaaatgcaa ttaaagtctt gggtatacat gacacccaga catttttttt agggtacgtc
     1021 cccaaaaaga gtgcagcgtt actcgccaaa acgggacttg ttgaattggt ttacccaaga
     1081 ttacttagaa ttgcgaccga tggagaccac gacatcaaaa ttaattacac cttagtcggc
     1141 cccaagaatg acaaacacaa atacaataat tatttgaatg aacagcctgc atctaaagag
     1201 cagaaagact acttaacctt ctttagtatc cccataagca aaggactgaa ttctgggcaa
     1261 gcggaagcgt taattagtga acatgaaaaa acggctagcg acttcgagct agatgagtgg
     1321 aataattatc aaaatattct ggaatatttt aaagatgacc ttcgggaaac cgacggaatc
     1381 aaaaaggtga gcgaacccct gctgattgag gcgctgaagc aactgaaaca ggaagggaat
     1441 tcctacgagc ggctgtacga ggatatcgac gaagtcgttg agcggttaat cgaaataaaa
     1501 cccgaactag cctgctaatt cgggtttttt cgatccacgc gagccacaac atcattcaga
     1561 ttttccaaca aattctgtcg tccctgggtt aattctctag cctgctaccg cgaccgacta
     1621 accaacacta tcgccgttca cgccgttggc tgactacaaa gatttggccg aatggcggcc
     1681 tgctcgatag gccgcctcga gggccgcttt aaggttccac accgcaatgt cgtgaaaatc
     1741 cagcgcgtcg cggttgcgcg tagccaaggt ctcaataccg aaatagtggc tagcgatgtg
     1801 agtgagcttg gcgtctggga tgggggtttg ggcggcatct acagcagcgt caggggtgtt
     1861 catcttcgat cctcagtgcg taatgtgatg gctccatgta cacgtcttcg gctctcaaaa
     1921 gcaagcatta tgtccattaa aatcaatacg ttattaccat aagaggctac cgcaatcggc
     1981 atctccatcc gcgcctacgc ccgccatcgc ggcatcagcg ataccgccgt acacaaggcc
     2041 atccgcactg gccgtatcac ccctgaagcc gacggcacac tcgaccctga acgcgcagat
     2101 cgcgagtggg cactcaatac cgaagcgctc cggt
//
LOCUS       DAOQGS010000689         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_34887_length_2130_cov_4.617831, whole genome shotgun sequence.
ACCESSION   DAOQGS010000689 DAOQGS010000000
VERSION     DAOQGS010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34887_length_2130_cov_4.617831"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2014
                     /gene="uvrD"
                     /locus_tag="PKZ52_17790"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             <1..2014
                     /gene="uvrD"
                     /locus_tag="PKZ52_17790"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HNG61680.1"
                     /translation="HQIMAVTFTNKNAKEMRERLQATLGDASSGAFRNLWVGTFHSIA
                     HRLLKTHWQDAGLPQNFQILDSDDQLRLIKRVSQALNLDEERWPAKQAQWFINEQKDE
                     GVRPAHIVEGADVYLATMKAVYQGYEETCQRGGMVDFAELLLRSHELWLTKPALLKHY
                     QQRFKHILVDEFQDTNAVQYAWLRVLAGNSAQVTAVGDDDQSIYGWRGARIENIQQFD
                     SHFPATQTTRLEQNYRSSKTILEAANTVIANNQGRLGKNLWTEDQQGEPIDLYAAFNE
                     QDEARFVVERIESWVAQGNPRQSAAILYRSNAQSRVLEEALIREGVPYRIYGGQRFYE
                     RLEIKNALAYLRLLQNRQDDAAFERVLNTPTRGIGSKTEEILRQSARDQNISLYQAAL
                     GLIKNNVLPNRAASALAGFLSLLSKLETEIKELPLAELVAHVINESGLLQFHQNEKGE
                     RGQARVENLQELVNACGAFKADDGLEEQTELAAFLDLASLDAGEAQAQDFEDAVQLMT
                     LHSAKGLEFPLVFLVGMEEHLFPHKMSADTKEGLEEERRLCYVGITRAMKKLILTYAE
                     SRRLYGSETHNPPSRFLREIPSELIQEVRVKSTIVIPANQRFKQQDISDTPGLRLGQS
                     VNHSVFGEGVILQFEGQGPAARVQVAFESAGTKWLVAQYAKLEALN"
BASE COUNT          540 a          563 c          593 g          434 t
ORIGIN      
        1 ccatcaaatc atggcggtga ccttcaccaa taaaaacgct aaagaaatgc gcgagcggct
       61 gcaggctacc ctgggggacg ccagtagcgg cgcctttcgc aacctctggg tgggcacctt
      121 tcactccatc gcccaccgct tgttaaaaac ccactggcag gatgcggggc tgccgcagaa
      181 ttttcagatt ctcgactccg acgaccaatt gcgcctaatt aaacgggtca gccaagccct
      241 caatctggac gaggagcgtt ggcccgccaa acaggcccag tggtttatta acgaacaaaa
      301 agacgagggc gtgcgcccgg cgcacattgt ggaaggggcg gatgtgtacc tggccaccat
      361 gaaggcggtg taccagggtt atgaggagac ctgccagcgc ggcggcatgg tggattttgc
      421 tgaattgctg ctgcgctccc acgagttgtg gctcaccaaa cccgccctgc tcaaacacta
      481 tcaacagcgc tttaaacata ttttggtgga cgaattccaa gacaccaacg ccgtgcaata
      541 cgcctggttg cgggtgctgg cgggcaacag cgcccaggtg acggcggtgg gggatgacga
      601 ccaatccatt tatggctggc gcggcgcccg tattgaaaat atccagcagt tcgacagcca
      661 cttccccgcc acccaaacca cgcgcctgga gcaaaactat cgctccagca aaactatttt
      721 ggaggcggcc aatacggtta tcgccaataa ccaaggccgc ctgggtaaaa acctctggac
      781 cgaggatcaa cagggcgaac ctatcgacct ctacgccgcc tttaacgagc aggacgaggc
      841 gcggtttgtg gtggagcgca tcgaaagctg ggtggcccag ggcaacccgc gccaaagcgc
      901 ggctattttg taccgctcca acgcccaatc ccgagtattg gaagaagcct taattcgcga
      961 aggcgtgccc tatcgcattt acggcggcca gcgcttctac gaacggctgg aaattaaaaa
     1021 tgccctcgcc tatttacgcc tattacaaaa ccgccaggac gacgccgcct tcgagcgggt
     1081 gctcaacacc cccacgcggg gcatcggcag taaaacggag gaaattctgc ggcagagtgc
     1141 gcgggaccaa aatatttccc tctaccaagc ggccttgggg ttaattaaaa acaatgtgct
     1201 gcccaaccgg gcggccagtg ccctggcagg gtttttaagt ttattaagta aattagaaac
     1261 cgaaataaaa gaattacccc tagctgaatt agttgcccat gtcattaatg aatccggctt
     1321 gttgcaattt catcaaaatg aaaaaggcga gcggggccag gcgcgggtgg aaaacttaca
     1381 agaattagtt aacgcctgcg gcgcttttaa agccgacgac ggattggaag aacaaacgga
     1441 attagccgcc tttttagacc tcgcctcttt agatgcaggc gaagcccaag cgcaggattt
     1501 tgaagacgcg gtgcaattaa tgaccctgca cagcgccaaa ggcttggaat tcccgctggt
     1561 atttttagtg ggcatggaag agcatctctt cccccataaa atgtctgccg ataccaaaga
     1621 gggtttagag gaagagcgcc gcctgtgtta tgtgggcatc acccgcgcca tgaaaaaact
     1681 cattctcacc tacgctgaaa gccgccgcct ctacggcagc gaaacccaca acccaccctc
     1741 gcggttttta cgggaaattc ccagcgaatt gatccaagaa gtgcgcgtga aatccaccat
     1801 tgtaattccc gccaaccagc gttttaaaca acaggatatt agcgatactc cgggcttgcg
     1861 cttggggcag agcgttaatc actcggtatt tggggagggg gtgattttgc agtttgaagg
     1921 ccaggggcca gcggcgcggg tgcaggtggc gtttgagagt gcggggacta aatggttggt
     1981 ggcgcagtat gcgaagcttg aggcgttaaa ttaaaaattg atgttaaata cctgttgaat
     2041 tggttggacg ggcaggaaac actcgatcgg gtgttttagg gaacctctga aaaatgtagg
     2101 cggcgaagcg ctggctaggc gaatttgcca
//
LOCUS       DAOQGS010000690         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_35176_length_2120_cov_4.079419, whole genome shotgun sequence.
ACCESSION   DAOQGS010000690 DAOQGS010000000
VERSION     DAOQGS010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35176_length_2120_cov_4.079419"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKZ52_17795"
     CDS             <1..290
                     /locus_tag="PKZ52_17795"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG61681.1"
                     /translation="NREFGSYLDNLHVAAAHQGKGIGKALVQAVANSCVHAAQSEGLC
                     LLVNQTNARAQNFYLNLGAVNARADTWAAPDGSAVPTFWFIWPQVNALLAH"
     gene            360..1067
                     /gene="tsaB"
                     /locus_tag="PKZ52_17800"
     CDS             360..1067
                     /gene="tsaB"
                     /locus_tag="PKZ52_17800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNG61682.1"
                     /translation="MLTILAIEASAEACSVALTFRGLVFARTINRLKSHSLVILPAVD
                     ELLAEANCAPSDIDAIAYDCGPGAFTGLRIGLSFAQGLAMGWRKPLVPVASLACLAHQ
                     TINLRPELNQVVALLDARMSELYAAVYRRAADGFTPIKEAFLCSYAQADELLGTYSGA
                     DCALVGLGVRETSAALKAQFVFVDATMNPEASALLVLAELAFRQGQAVPVEQAELVYV
                     RNSVTWDKHQLRRNRDQ"
     gene            1077..1871
                     /locus_tag="PKZ52_17805"
     CDS             1077..1871
                     /locus_tag="PKZ52_17805"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNG61683.1"
                     /translation="MENIIQVIILGLIEGFTEFLPISSTGHLIIAQQLGLGERSDLFN
                     VVIQAGAISAVTLIYWRRILSLFLNFKEPANKDYLLKLGVAFLITAILGVLVKKFGIE
                     LPDSTRPVAIALILGGFWILWAEWYTAKKTPSPDMTWAGAIVVGFAQIVAAIFPGTSR
                     SGAGIFAAMLAGNPSRAAAADFVFMLGIPTMYAASAKKIMDAAQDGLIAQEAWGDVAL
                     GFVVAMVSAFFVVKWLLGYIKSHRFTGFAIYRIILGIVLLTFFSQY"
     gene            1911..>2120
                     /locus_tag="PKZ52_17810"
     CDS             1911..>2120
                     /locus_tag="PKZ52_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNG61684.1"
                     /translation="MNKCLPGLLDFLPAAPSPYHAVEQIKQALTQAGFIQLDEKAPWA
                     LSPGGCYFIVRREASLLAFVYGKAPL"
BASE COUNT          419 a          545 c          591 g          565 t
ORIGIN      
        1 aaaacagaga atttggttct tatttggata atctgcatgt ggctgcggcg caccagggca
       61 aggggatagg caaggcgcta gtgcaggcgg tggcgaattc gtgtgtgcac gcggcgcaga
      121 gcgagggttt gtgtttattg gtgaatcaaa ctaacgctcg cgcccaaaac ttttatttaa
      181 acctcggcgc ggtaaatgcg cgggcggata cctgggcggc gccggatggc agcgctgtgc
      241 cgactttttg gtttatctgg ccccaagtaa acgctttact ggcgcactaa ccaacctcgg
      301 ccacagggct tgcatgtgcc ccttaagggc gtaaaatccg gcactttttc gaggggccta
      361 tgcttaccat tctcgccatt gaggccagtg ccgaagcctg ctccgtggcc ctcaccttta
      421 gagggctggt gtttgcccgc accatcaaca ggcttaaatc ccactccctg gttattctgc
      481 ccgccgtgga tgaactctta gcggaggcca attgcgcccc tagtgatata gacgccatcg
      541 cctatgactg cgggccgggc gcttttaccg gtttgcgcat cggtttaagt ttcgcccagg
      601 gtttggccat gggctggcgt aaacccctgg tgccggttgc cagcctcgcc tgcctcgctc
      661 accaaacaat taacctgcgg cctgaattaa atcaggtggt ggctctgttg gatgcccgta
      721 tgagcgagtt atacgcagcg gtttatcgcc gcgctgccga tgggtttacc cctataaaag
      781 aagcattttt atgctcctat gcccaggccg atgagctgct cggtacttat tctggtgccg
      841 attgcgcact ggtgggttta ggcgtgcggg aaacctccgc cgccttaaag gcacaatttg
      901 tttttgtgga tgccactatg aacccggagg ctagcgctct attggtattg gctgaattgg
      961 cctttcggca gggccaagcc gttcctgtag agcaggctga attggtatat gtgcgcaatt
     1021 cggttacttg ggataaacat caactccgtc gcaaccgcga ccaataagag gcacgtgtgg
     1081 aaaatattat tcaggtgatt attttagggc tgattgaagg ttttaccgaa tttctgccca
     1141 tctccagcac cgggcattta attattgccc agcaattagg cttaggagag cgctcggatt
     1201 tatttaatgt ggtgattcag gctggggcta tttcggccgt taccttaatt tattggcggc
     1261 ggattttatc gctgtttttg aattttaaag agccagccaa taaagactat ttattaaagc
     1321 tgggggtagc atttttaatt accgccattt tgggtgtgtt ggtaaaaaaa ttcggcattg
     1381 aattgcccga ttccacccgc ccggtggcca ttgcgttaat tctcggtggt ttttggattt
     1441 tatgggccga gtggtatacc gccaaaaaaa cacccagccc ggatatgacc tgggcggggg
     1501 ccatagtggt gggctttgcg cagattgtgg cggccatctt ccccggcact tcccgctccg
     1561 gcgcgggtat ttttgccgct atgctggcgg gcaaccccag ccgggcagcg gcggcggatt
     1621 ttgtatttat gttgggtatt cccaccatgt acgcggccag cgcaaaaaaa attatggacg
     1681 ccgctcaaga cggcttaatc gcccaggagg cctgggggga tgtggccctg ggctttgtgg
     1741 tggccatggt atcggcgttt tttgtggtga agtggctgct tggctatatt aaatctcacc
     1801 ggtttaccgg ttttgccatt taccgaatca ttctaggcat agtgctgctg acgtttttct
     1861 cccagtacta aacccgccat tctcgcctta gcccctgcgg agcatccagt atgaataaat
     1921 gccttcccgg cctgctcgat tttttacccg ccgccccctc gccctaccac gcggtggagc
     1981 agattaaaca ggcccttacc caagccgggt ttattcaact ggatgaaaaa gccccctggg
     2041 ccttaagccc cggcgggtgc tattttattg tgcgcaggga ggcctccctg ctggcctttg
     2101 tgtacggcaa ggcgccttta
//
LOCUS       DAOQGS010000691         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_35321_length_2115_cov_4.587864, whole genome shotgun sequence.
ACCESSION   DAOQGS010000691 DAOQGS010000000
VERSION     DAOQGS010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35321_length_2115_cov_4.587864"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKZ52_17815"
     CDS             complement(<1..349)
                     /locus_tag="PKZ52_17815"
                     /inference="COORDINATES: protein motif:HMM:NF023154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HNG61685.1"
                     /translation="MAYLSFAGAITVGGNTWLVNARKSGIYTEMAKRMIGQIEAMLTH
                     HSKIHIVRFDLHQPDYTPDNSRISLFNRYLHNRLKTRYKLARIGFVWVREQERAKQQH
                     YHYALILDGHKIHH"
     gene            complement(620..835)
                     /locus_tag="PKZ52_17820"
     CDS             complement(620..835)
                     /locus_tag="PKZ52_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61686.1"
                     /translation="MGRYKIEVTEHTAYSVTLEADSPQEALEAAQRHEGESVQAAAPS
                     WTFGKPKRMETYREFSETLETKKTAKR"
     gene            complement(838..1167)
                     /locus_tag="PKZ52_17825"
     CDS             complement(838..1167)
                     /locus_tag="PKZ52_17825"
                     /inference="COORDINATES: protein motif:HMM:NF025456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG61687.1"
                     /translation="MSKKNGRTPKRATTQKAAQQKPASYKKLPLNSTDRRAQQQRLLK
                     TLIEKGSITTIEARQELNVMSPAPRILELREQGNRIFTQFVTITDQHGFMHRGVAKYV
                     LISLAGV"
     gene            complement(1171..1386)
                     /locus_tag="PKZ52_17830"
     CDS             complement(1171..1386)
                     /locus_tag="PKZ52_17830"
                     /inference="COORDINATES: protein motif:HMM:NF017717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HNG61688.1"
                     /translation="MNQPQTGVLRLKTILGDPAAKPPIPPLIPVSKSTWWKGVKDGTY
                     PQPVKIGARCTAWKAADIYAYINGLGV"
     gene            complement(1485..>2115)
                     /locus_tag="PKZ52_17835"
     CDS             complement(1485..>2115)
                     /locus_tag="PKZ52_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61689.1"
                     /translation="EAIFPSGAGVLHNSTKSPKISGVWDLLMWGGERLFVKRQFHSLM
                     NDGAVLDVSTFDGSYVQSYDRELVFSLQEFFGDIDGYPSDDLIRPLSHPNNYCPMDGL
                     PKDSFLVIRTEALSEFENNLRFKEKSEMRTPKDTLVEALGLMTLLLAETSPNLKHGNK
                     PNFSQIKERIKLKAQQVFKLKGGETYPNLENINRDLKSAVEKLEAQAPK"
BASE COUNT          472 a          482 c          508 g          653 t
ORIGIN      
        1 ggtgatgtat tttgtggccg tccagaataa gtgcatagtg gtaatgctgt tgtttggccc
       61 gctcctgctc gcgcacccaa acaaaaccta tgcgggcgag tttatagcgg gtttttaagc
      121 ggttgtgcag gtagcggtta aataggctga ttctgctgtt atcgggcgtg taatccggtt
      181 ggtgtaagtc aaaacgcact atatgtattt tgctgtggtg cgtgagcatc gcctcaattt
      241 ggccaatcat gcgcttagcc atttcggtat aaatgccact tttgcgcgcg ttaactaacc
      301 aagtattgcc gcccaccgtt atggccccag caaaacttaa ataggccata gagaggggat
      361 tactgggtat ttagatatta tgaatatcct agtggtaaaa accgctacag ctaaggccgg
      421 gcgcgggtta gcggcgattt taccagcctg aaaaacccct aaatgttgtc cagcaccgtc
      481 caagtgctct aggttattga ttttaaataa taaaaatccg gccttggccc tgtgcttgct
      541 catggtgtaa cctgcaattg gctggcagcg cggtcgccaa acttttgctg ccagccagtt
      601 taatgaaagc cgctaggggt tatctcttag cggttttttt tgtttccaag gtttcagaaa
      661 attccctgta agtttccatg cgtttaggtt tgccaaaagt ccatgaaggg gctgcggcct
      721 gcactgattc accttcgtgc cgttgggccg cttccagtgc ctcctgtggg ctatctgcct
      781 caagcgttac gctataggca gtgtgttctg tgacttcaat tttgtagcgt cccatactta
      841 aacccctgcc agactaatca gcacgtattt ggcaacacca cggtgcataa atccatgctg
      901 atcagttatc gtcacaaact gggtaaaaat acggttgccc tgttcgcgca gctccaaaat
      961 tcgcggcgcg gggctcatta catttaattc ctggcgcgcc tctatcgtgg tgattgatcc
     1021 tttttcaata agcgttttga gtaggcgttg ctgctgggcg cgcctatcgg tactattcaa
     1081 agggagtttt ttatagcttg ccggtttttg ttgggcggct ttttgagtgg ttgcacgctt
     1141 cggggtgcgg ccattttttt tgctcatggc ttacaccccc aggccgttga tataggcgta
     1201 aatatctgcc gccttccagg cggtacagcg ggcacctatt tttactggct gtgggtaggt
     1261 tccatctttt acacccttcc accaagtcga tttagataca gggattagtg gagggatcgg
     1321 cggtttggca gcgggatcgc ctagaatggt ttttaggcgt aaaacgccgg tttgtggttg
     1381 gttcataccg tcttttcctc tgttgtttgg tacaggcaga ggttaggagg ttttgatttt
     1441 ttatgggtgg ccagccaaaa actcgtttgg ccggacagca cttattattt tggtgcttgg
     1501 gcttctaatt tttctacagc actttttagg tctcgattga tgttctcaag gttgggataa
     1561 gtctcacccc ctttgagctt aaacacctgc tgtgccttta attttatcct ttccttaatt
     1621 tgagaaaaat taggcttatt gccatgtttc aagttggggg atgtttccgc aagaagcaac
     1681 gtcataagcc caagagcctc aacaagcgta tcttttggag tcctcatttc tgatttttcc
     1741 ttaaatctca ggttgttctc aaattcactt aatgcttctg tgcgaattac taaaaaactg
     1801 tcttttggta gtccatccat tgggcaataa ttgttgggat gtgagagtgg tcggattagg
     1861 tcatcactgg gatagccatc aatgtcccca aagaactctt gtaatgaaaa taccaattcg
     1921 cgatcgtaac tttgcacgta tgatccatca aaagtgctca cgtctagtac cgccccgtca
     1981 ttcattagtg agtgaaattg ccgttttaca aatagtcttt cgcctcccca catcaacaaa
     2041 tcccaaaccc cactaatttt tgggctcttc gtagaattgt gtaaaacccc cgcgccagat
     2101 ggaaaaatgg cctcc
//
LOCUS       DAOQGS010000692         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_35643_length_2103_cov_3.882812, whole genome shotgun sequence.
ACCESSION   DAOQGS010000692 DAOQGS010000000
VERSION     DAOQGS010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35643_length_2103_cov_3.882812"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKZ52_17840"
     CDS             <1..401
                     /locus_tag="PKZ52_17840"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNG61690.1"
                     /translation="IPQLTQRHQQRAAVKPDYVFLEEQIKLAAEIKAKKTVSLNEKVR
                     EQEKTDMESKSMALENKRRKIKGEPEYKTLAEYRAAQKAEEDKAEAEQSTPHSKIDPT
                     KDALLTEAGAILVDFSKLLKEQSQKKVANF"
     gene            465..1289
                     /gene="xthA"
                     /locus_tag="PKZ52_17845"
     CDS             465..1289
                     /gene="xthA"
                     /locus_tag="PKZ52_17845"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNG61691.1"
                     /translation="MKCISFNTNSIRIRLHQLEETIRVHAPDVIGIQETKVTDTEFPV
                     AEINALGYHCEFIGQKTHYGVALLSKVPPITVTKGFITDGADAQRRFIAAQFNSQDGK
                     PVHLLNGYFPQGESRDHPVKFPNKEKFYQDILAHLGNHGANERVILMGDMNISHQDCD
                     IGIGEENRKRWLKTGKCSFLPEERVWLNRLIEWGLVDTFRSLNPDTQDRFSWFDYRSK
                     GFEAEPKRGLRIDLILASKGLVPSLTDAGIDYEIRGMDKPSDHCPIWAEFKDIKLV"
     gene            complement(1330..1821)
                     /gene="ilvN"
                     /locus_tag="PKZ52_17850"
     CDS             complement(1330..1821)
                     /gene="ilvN"
                     /locus_tag="PKZ52_17850"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNG61692.1"
                     /translation="MRRIISVLMENEPGALSRVVGLFSQRGYNIESLTVAPTEDATLS
                     RMTLTTIGDDHKIEQITKHLNRLIDVVKLVDLTEGAHIERELMLIKVRASGAQRAEIK
                     RCVDIFRGQIIDVTASVYTIQLTGTSEKLDAFYEAVGEASILEVVRSGVSGIARGEKV
                     LGL"
     gene            complement(1821..>2103)
                     /locus_tag="PKZ52_17855"
     CDS             complement(1821..>2103)
                     /locus_tag="PKZ52_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNG61693.1"
                     /translation="KQWQDMQYNSRYSESLYEESLPDFVKLMEAYGHVGIRVNKREEL
                     RAKMEECFAMKDKTVFMDIYVDPTEHVYPMHIAPNGSMRDMWLNKTERT"
BASE COUNT          567 a          492 c          513 g          531 t
ORIGIN      
        1 ttattccgca gttaacccag cgccaccaac agcgggcagc ggttaaaccc gactacgtat
       61 ttttggaaga acaaattaaa ttggctgctg agattaaagc taagaaaact gtctctctaa
      121 acgaaaaagt gcgcgagcag gaaaaaaccg atatggaaag caaaagtatg gcgctagaaa
      181 ataaacgccg taagattaaa ggcgagcctg aatataaaac gcttgcggaa taccgagcgg
      241 cacaaaaagc tgaagaagat aaagcggaag cggaacaatc aaccccgcac agcaaaatcg
      301 accccactaa agatgcatta cttactgagg ctggtgccat tttggtggac ttttccaagc
      361 tgcttaaaga gcagagccag aaaaaagtcg ccaattttta atcgcactgc aattattctt
      421 catagcccgg caatgccggg cttatttttt taaggcaccc accgatgaag tgtatttcgt
      481 ttaataccaa cagcattcgt attcgccttc accaattgga ggagaccatt cgcgtccacg
      541 ctcccgacgt gatcggcatt caagaaacca aggtcacgga tacggaattc ccggtggcag
      601 aaattaatgc gcttggctat cactgtgagt ttattggcca aaaaacccat tatggagtag
      661 cgctgttatc taaggtgccc cctataaccg tcactaaagg ttttataacg gatggcgccg
      721 acgctcagcg gcgttttatc gccgcccaat ttaatagcca agatggcaaa cccgtacatc
      781 ttctcaacgg ttatttcccg cagggggaaa gccgcgacca cccggtgaaa tttcccaaca
      841 aagaaaagtt ttatcaggat attttggccc acttaggcaa tcacggggcg aatgagcggg
      901 tgattttaat gggcgatatg aatatatccc accaggattg cgatataggc attggcgagg
      961 aaaatcgcaa gcgctggtta aaaaccggca aatgcagttt tttacccgaa gagcgcgtgt
     1021 ggttgaaccg attaatagaa tggggactgg tggatacctt tcgcagttta aaccccgata
     1081 ctcaggatcg ttttagctgg tttgattatc gcagcaaggg ttttgaggct gagcctaaac
     1141 gaggtctgcg cattgatctg attttggcga gcaagggatt ggtgccgagt ttgacggatg
     1201 ctggcatcga ctatgagatt cgagggatgg ataagccatc cgatcactgc cctatttggg
     1261 ctgagtttaa agatattaaa ttggtgtagg catccctgcc tgttaaaacc gtgggaaagt
     1321 gctgcaggct tatagcccca gcactttctc ccctcgggcg ataccggaaa cgccggagcg
     1381 taccacttct agaatcgagg cttcgcctac ggcttcgtaa aaagcatcga gtttttcgct
     1441 ggtgccagtt aattgaatgg tgtagaccga ggcggtgacg tcgataattt ggccgcggaa
     1501 aatatccacg cagcgtttta tctctgcccg ctgggcaccg gaggcgcgca ccttaattaa
     1561 catcagctcc cgctcaatat gggcgccttc ggttaaatcc accagcttca ccacatcaat
     1621 taagcgattt aaatgcttag tgatctgctc aattttatga tcgtcaccga tggtggtgag
     1681 ggtcatgcga gacagggtcg catcttctgt gggcgccaca gtgaggcttt cgatgttgta
     1741 accgcgctgt gaaaataaac ccactacgcg ggataaggcg ccgggttcgt tttccattaa
     1801 aactgaaata atacgtctca ttaggtgcgc tccgttttgt ttaaccacat atcgcgcata
     1861 gatccgttgg gtgctatatg catggggtat acgtgttcgg ttggatcgac gtaaatatcc
     1921 ataaatacgg ttttatcctt catggcaaaa cattcttcca tttttgcccg cagctcttcg
     1981 cgcttattta cgcgaatgcc cacatggccg taggcctcca ttaatttcac aaaatccggc
     2041 agggactctt cgtacaggct ctctgaataa cggctgttgt actgcatatc ttgccattgc
     2101 tta
//
LOCUS       DAOQGS010000693         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_35754_length_2099_cov_4.435910, whole genome shotgun sequence.
ACCESSION   DAOQGS010000693 DAOQGS010000000
VERSION     DAOQGS010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35754_length_2099_cov_4.435910"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="cca"
                     /locus_tag="PKZ52_17860"
     CDS             complement(<1..663)
                     /gene="cca"
                     /locus_tag="PKZ52_17860"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047302.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HNG61694.1"
                     /translation="MQIFLVGGAVRDQLLGLPVSEKDWVVVGASAAELLAQGFKAVGK
                     DFPVFLHPHSQEEYALARQERKTAPGYGGFSFDTQPSVSLEEDLVRRDLTINAMAQDD
                     KGTIIDPYGGQQDLQARLLRHVSPAFCEDPVRILRIARFSARYHSLGFKIAPETLHLM
                     GEMVRANEVDHLVPERVWKETSRALHETNPEVYFHVLRSCGALARLMPELDALFGVPQ
                     RAD"
     gene            950..1879
                     /locus_tag="PKZ52_17865"
     CDS             950..1879
                     /locus_tag="PKZ52_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNG61695.1"
                     /translation="MQLSSAYCAVLIIWATTPLGIKWSNSGLSFSSAITLRMLLALIL
                     CAAVLAVQKKRLIHSKKDWQAFGAGALGLFPNMLIVYWSAQYIPSGLVSILLGFFPFF
                     LGVFSWLLLRERSLNARQVAALCLAFCGLAVIYMQQVQTSWQALWAVLGVLVAAAFWA
                     LSSIWVKALGVEIDAMRQSTGTLLIAAPIFAVYWLLFDHQLPAHWQLKSQLGVVYLVV
                     AGSCLGHTLYFYVLRRCSLAATGLLTLLSPMVALGIGFLVLGETLSVRELIGVALVLM
                     ALGVYSGVLIGAWNLILLKLVRMGRWAAIKVTQ"
BASE COUNT          422 a          488 c          573 g          616 t
ORIGIN      
        1 gtccgcccgc tggggtacgc caaatagggc gtctaattct ggcatcaggc gggccagcgc
       61 gccgcagctg cgcagtacgt gaaaataaac ctccggattg gtctcgtgca gggcgcggga
      121 ggtttctttc cagactcgct cgggcactaa atgatccact tcgttggccc tcaccatttc
      181 gcccattagg tgcaaggtct caggcgcaat cttgaagcct aaagaatggt agcgcgcgct
      241 gaagcgggcg atgcgcagga tgcgcaccgg gtcttcacaa aatgcggggg agacatggcg
      301 caacagccgc gcctgcaaat cctgttggcc gccgtagggg tcgatgattg tgcccttgtc
      361 atcctgggcc atggcattaa tggttaaatc ccgccgcact aaatcctctt caaggctcac
      421 gcttggctga gtatcaaaac taaatccccc atagccaggc gctgttttgc gctcctgccg
      481 ggctagggca tattcctctt ggctgtgggg atgtaaaaat accggaaagt ctttgccgac
      541 ggctttaaaa ccctgcgcta ataattctgc ggcggatgca cctaccacca cccaatcttt
      601 ttcgctgacg ggcaggccta ataattgatc gcgcactgcg ccgccgacaa gaaatatttg
      661 catggggtgt tgaccttggg aattaattgc acgacactgt aattgaattt aatttcatcg
      721 acaaattact tatgtcttta cgcgctgcag ctgccaattt cgcttatacc gcttgggttt
      781 ttcctgtggc ggtatttgtt gtactagcgc atacgccagt gccagcgatt gcagcggtat
      841 ggcgccgtgc ccgatcggtg cgctgtaggc ttaggtagat tttttttatt actagagccg
      901 cagcacagtc tgcggctttt tttgcgcaga agctcggcag ggagatgtta tgcagctttc
      961 tagcgcctat tgcgccgtat taattatttg ggccacaact ccattgggga ttaagtggag
     1021 taatagcggt ttaagtttta gcagtgctat tacgctgcgt atgctgttgg cgttaatcct
     1081 ctgcgctgcc gtattagcag tgcagaaaaa gcgcttaatc cacagtaaaa aagattggca
     1141 agcctttggc gcgggcgctt tgggtttgtt tcctaatatg ctgattgtgt attggagcgc
     1201 gcaatatatt ccctccggtt tggtttccat tttactgggc ttttttccgt tctttttagg
     1261 tgtgttttcc tggttgttgt tgcgcgagcg ctcgctcaat gcacggcagg tggccgcatt
     1321 gtgtttggca ttttgtggtt tggcggtgat ttatatgcaa caggtacaaa cttcttggca
     1381 agccctgtgg gcggtattgg gtgtattagt ggcggcggcc ttttgggcgc tcagttccat
     1441 ctgggttaaa gccctaggcg tggaaattga cgctatgcgc caatccaccg gaaccctatt
     1501 aattgccgcg ccgatttttg ctgtttattg gctgttgttc gatcatcaat tgcctgccca
     1561 ctggcaatta aaatctcaat tgggcgtggt ttatctggtt gtcgcaggtt cctgcttggg
     1621 ccacacgctt tatttttatg tgttgcgccg ctgttcttta gcggccactg gccttttaac
     1681 tttgttaagt ccaatggttg ccctaggtat tggttttttg gtgttgggcg agacgctttc
     1741 cgtgcgggag ctaattggag tggccctggt gttaatggcc ctgggtgtat attctggggt
     1801 attgatcggc gcttggaatt taattttact gaaattagtg cggatgggcc ggtgggcagc
     1861 aattaaagtg acccagtgaa ttgattgggc gcagcttaaa ttcaaaagga tttgtaacta
     1921 ttcaggatct tattatctgt cttgaggtaa taaccgcact gacgggtacc aattatgtgc
     1981 gtgttttaac actgtggcac cgcaatactg cccgcagtgg taactacaga ttagtgcaga
     2041 cacgccgtga atacatccat gtaggctcgt caaaaaggtc cctctttttg acggtctgc
//
LOCUS       DAOQGS010000694         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_35821_length_2097_cov_3.984819, whole genome shotgun sequence.
ACCESSION   DAOQGS010000694 DAOQGS010000000
VERSION     DAOQGS010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35821_length_2097_cov_3.984819"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1783)
                     /gene="ubiD"
                     /locus_tag="PKZ52_17870"
     CDS             complement(317..1783)
                     /gene="ubiD"
                     /locus_tag="PKZ52_17870"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNG61696.1"
                     /translation="MSAQDLREFIAQLEARGQLKRIRHSISPVLEMTEIADRTLKAAG
                     PALLFENPNGFTIPVLANLFGTPERVALGMGQTSVAALKEVGELLAFLKEPEPPKGLK
                     DAWAKLPIFKQVLNMAAKEVSSAACQEVVYEGAAVDLTQLPIQTCWPGDVAPLITWPL
                     VITRGPHKERQNLGIYRMQLLGPNKLIMRWLSHRGGALDFREWQQAYPGEHFPVSVAL
                     GADPATILGAVTPVPDTLSEYAFAGLLRGSKTQVVKSLTNSLQVPAGAEFILEGYLAP
                     GEMADEGPYGDHTGYYNEVERFPVFTVTTLTHRKNPIYHSTYTGRPPDEPAILGVALN
                     EVFVPLIKKQFPEIVDFYLPPEGCSYRMAVVSIKKQYPGHAKRIMLGVWSYLRQFMYT
                     KFVVITDDDINVRSWPDVIWAITTRMDPSRDTTLIDHTPIDYLDFASPVAGLGSKMGL
                     DATNKWPGETAREWGLPITMDAKVKTKIDAIWQELGIV"
     gene            complement(1866..>2097)
                     /gene="rho"
                     /locus_tag="PKZ52_17875"
     CDS             complement(1866..>2097)
                     /gene="rho"
                     /locus_tag="PKZ52_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275024.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNG61697.1"
                     /translation="HLDRKMAERRLYPAINIRRSGTRREDLLMGEGELHRVWILRKLL
                     NDMEDVAATEFLVDRLKNFKTNDEFFLSMKGK"
BASE COUNT          552 a          575 c          466 g          504 t
ORIGIN      
        1 tttaaatcca ccaccgcctt taaaaattcc tgccgcgcac aggcattgtg tgcctccagc
       61 aaactgtggc acaccacaac ctcaaaaccg cgccgcacca atgcccgctg taacacctgg
      121 caaaacgcct cgtcgtcatc aacaattaat aggggttctt tggtggctaa catgtcaaca
      181 caggtcttag aatattatta agtattagca gcttgttgaa atacggaggc ggcgacgcac
      241 caccaaggta gtatttcaac aagctgctag aaggccacac catgcgccga gtgcacaagc
      301 gtcgctacaa acgccatcac acaatcccta gctcctgcca aattgcatca atttttgttt
      361 ttaccttcgc atccatagtg ataggtagcc cccattcccg cgctgtttcc ccaggccatt
      421 tattggtggc atccaacccc attttagaac ccaagcccgc caccggagag gcaaaatcca
      481 aataatcaat gggcgtatgg tcaattaatg tggtatcgcg gctggggtcc atgcgggtgg
      541 tgatggccca aatcacatcc ggccaggagc gcacattaat atcatcatca gtgatcacca
      601 caaatttagt gtacataaat tgccgcaaat aagaccacac gcccaacata atgcgcttag
      661 catgccccgg atattgtttt ttaatactca ccaccgccat gcggtaggaa cagccctctg
      721 gcggcaaata aaaatcgacg atttccggaa attgtttttt aattaaaggc acaaacactt
      781 catttaatgc cacccctaaa atcgccggtt catccggcgg tcgtccggta taggtgctgt
      841 ggtaaatggg gtttttacga tgtgtaagtg ttgttacagt aaataccggg aaacgctcca
      901 cctcattgta ataacccgtg tggtccccat aggggccttc atcggccatt tcgccgggcg
      961 ctaagtagcc ctctaaaata aattccgccc ccgcaggcac ctgtaaggaa ttagtcaggc
     1021 tttttaccac ctgggtttta ctgccgcgca acaggcctgc aaaggcatat tcacttagag
     1081 tatccggcac cggagttacc gcccctaaaa tggtggcagg gtccgcacct aaagccaccg
     1141 acacagggaa atgctctcct ggataggcct gctgccactc gcgaaaatcc agcgcaccgc
     1201 cgcgatggct caaccagcgc ataattaatt tattcggccc caataattgc atccggtaaa
     1261 tgccgaggtt ttgccgctct ttgtgggggc cacgggtaat taccaaaggc caggttatta
     1321 agggcgccac atcgccaggc cagcaggttt gaatgggcaa ttgtgttaaa tctaccgccg
     1381 cgccttcata caccacctcc tggcaagcag cactgctaac ctctttggcc gccatattga
     1441 gcacttgttt aaatatcggc aattttgccc aagcatcttt tagccccttg ggtggctctg
     1501 gttcttttaa aaaggccaat aattccccca cctctttaag tgccgccaca gaggtttgcc
     1561 ccattcctaa agccacgcgc tccggcgtgc caaataaatt tgccagcaca ggaatagtaa
     1621 atccattggg gttttcaaat aacagcgcag ggccagccgc ttttaaggtg cggtcggcaa
     1681 tttccgtcat ttccaacacc gggctaatgg agtggcggat gcgttttaat tggccacgcg
     1741 cttccaactg agcaataaat tcacgcaaat cttgagcaga catatcaggg caccaaattt
     1801 agggcaaaaa aaatctcgca attgtactga gcacaaactg cgagattttt acgaattaaa
     1861 acggcttact tgcctttcat ggacaagaaa aactcatcat tggtcttaaa gtttttcaaa
     1921 cgatccacca agaattcggt ggcggcaaca tcttccatat cgttgagcaa tttgcgcaga
     1981 atccacacgc ggtgtaattc gccctcgccc atcaacaggt cttcacggcg ggtgccggag
     2041 cggcggatat taatggcagg gtataaacgg cgctctgcca ttttacgatc caggtgt
//
LOCUS       DAOQGS010000695         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_36181_length_2085_cov_3.927094, whole genome shotgun sequence.
ACCESSION   DAOQGS010000695 DAOQGS010000000
VERSION     DAOQGS010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36181_length_2085_cov_3.927094"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKZ52_17880"
     CDS             <1..260
                     /locus_tag="PKZ52_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61698.1"
                     /translation="EQPLIPTLKHILSTGNPYVFPLAHSTKPLYLVSASKTNTIYGGG
                     EPFSLSVICQDLTGYQQFDMAPAIALGRPVSPFTTAPNQPL"
     gene            309..>2085
                     /locus_tag="PKZ52_17885"
     CDS             309..>2085
                     /locus_tag="PKZ52_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNG61699.1"
                     /translation="MHSTADETALSSPKVGLDASSQVQATAPGQLRVIKRNGQVVPYT
                     DDKIAVAMTKAFLAVEGGTAAASRRVHETVEKLTQQIGATFRRRMPSGGTIHIEEIQD
                     QVELILMRTGQHKVARDYVIYRAERAKLREAKRALAQPIDYPPISVKNPDGSLEPLNQ
                     NRLEILVTEACAGLKGVDASAILKEAMRNLYDGVSADDVNTALVISARTLVEQEPNYS
                     YATARLLLDKLRSEALSFLGIASSATQSQMSDYYPQALPAYIARGIELELIDPALAGF
                     DLEQLGAAILPERDLQFTYLGLQTLYDRYFIHSNGTRIELPQVFFMRVAMGLALREEA
                     KNERAIEFYQLLSSFDYMSSTPTLFNSGTLRPQLSSCYLTSVPDDLHGIYGAIQDNAM
                     LSKFAGGLGNDWTPVRSLGAYIKGTNGKSQGVVPFLKVANDTAVAVNQGGKRKGAVCA
                     YLETWHLDIEEFLELRKNTGDDRRRTHDMNTANWVPDLFMKRVFDDKEWTLFSPNNVP
                     DLHDLFGAAFEARYEEYERMAAEGKIKPAKTVRAVDLWRKMLGMLFETGHPWITFKDP
                     CNLRSPQQHAGVVHSSNLCTEITLNT"
BASE COUNT          487 a          707 c          511 g          380 t
ORIGIN      
        1 gtgagcaacc acttatcccc acgcttaagc acattttatc cacaggaaat ccctacgttt
       61 tcccgcttgc acattccacc aaaccgcttt atcttgtgtc cgcctctaaa acaaacacaa
      121 tatatggggg tggtgagcca ttctccctaa gcgtgatctg tcaagacttg acaggttatc
      181 aacagtttga catggccccc gcaatcgcgt tgggccgccc agtttcgccg ttcacaacgg
      241 cacctaacca gccactttaa ttcacgcaac ctgcggcagt tcgctgcccg ttttctatgg
      301 agcactctat gcacagcacc gcggacgaaa ccgcattatc cagcccaaaa gtcggtttgg
      361 atgccagttc tcaggttcag gccactgccc caggccagct gcgcgtgatc aagcgcaacg
      421 gccaagtagt gccctacact gacgataaaa tcgccgtggc catgaccaaa gcctttttgg
      481 ccgttgaagg cggcactgca gcggcctccc gccgggtgca cgaaaccgtg gaaaaactca
      541 cccagcagat cggcgccacc ttccgccgcc gcatgccctc cggcggcacc attcacattg
      601 aagaaattca ggaccaggtt gaactgatcc tgatgcgcac cggccaacac aaggtggccc
      661 gcgactacgt gatctaccgc gccgagcgcg ccaaactgcg ggaggccaag cgcgccctgg
      721 cccagcccat cgactaccca cccatcagcg tgaaaaaccc cgacggcagc ctggagcccc
      781 tcaaccaaaa ccgcctggaa attctcgtta ccgaagcctg cgcgggcctt aaaggtgtgg
      841 acgccagcgc catcctcaaa gaagccatgc gcaacctcta cgacggcgtc tccgctgacg
      901 atgtgaacac cgctctggta atctccgccc gcaccctggt ggagcaggag cccaactaca
      961 gctacgccac cgcccgcctg ctgctggata aactgcgcag cgaagcactc agctttttgg
     1021 ggattgccag cagcgccacc caaagccaaa tgagcgacta ctacccgcaa gccttgcccg
     1081 cctacatcgc ccgcggcatt gaactggaac ttatcgaccc cgccctggca ggcttcgacc
     1141 tggagcagtt aggcgccgcc atactgcccg agcgggattt gcaatttact tacttaggcc
     1201 tgcaaaccct ctacgaccgc tacttcatcc acagcaacgg cacccgcatc gaactgccgc
     1261 aagtattttt tatgcgcgtc gccatgggct tagccctgcg ggaagaagcc aaaaacgagc
     1321 gcgccattga gttctaccaa ctgctctcaa gcttcgatta catgagcagt acacccacct
     1381 tatttaactc cggcaccctg cgcccgcagc tctccagctg ttacttgacc agcgtgccgg
     1441 acgacctgca cggcatctac ggcgccatcc aagataacgc tatgctgagc aaattcgccg
     1501 gtggcctggg caacgactgg acccccgtgc gctccctcgg cgcctatatc aaaggcacca
     1561 acggcaaatc ccagggtgta gtgcccttct taaaagtcgc caacgacaca gctgtagcgg
     1621 taaaccaagg cggcaagcgc aagggcgcgg tatgtgccta cctggaaacc tggcacctgg
     1681 atatcgaaga gtttttagag ctgcgcaaaa acactggcga cgaccgccgc cgcacccacg
     1741 acatgaacac cgccaactgg gtgcccgacc tatttatgaa gcgcgtgttt gacgacaaag
     1801 agtggaccct attctccccc aacaacgtgc cggacctgca cgacctgttc ggcgccgcct
     1861 ttgaagcccg ctatgaagag tacgagcgca tggccgccga gggcaaaatc aaacccgcca
     1921 aaaccgtgcg cgcggtagac ctgtggcgca agatgctggg catgttgttt gaaaccggcc
     1981 acccctggat aaccttcaaa gacccctgca acctgcgcag cccgcagcag catgccggtg
     2041 tggtgcactc ctccaacctc tgtaccgaga tcacccttaa cacta
//
LOCUS       DAOQGS010000696         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_36806_length_2064_cov_3.584868, whole genome shotgun sequence.
ACCESSION   DAOQGS010000696 DAOQGS010000000
VERSION     DAOQGS010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36806_length_2064_cov_3.584868"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="mreC"
                     /locus_tag="PKZ52_17890"
     CDS             complement(<1..886)
                     /gene="mreC"
                     /locus_tag="PKZ52_17890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNG61700.1"
                     /translation="MALLAVAAVALALVYTQTQWLGPLKQKAIDIATPFYWLANMPRE
                     MSAWSQQRLVPKEQLLQENEALRTELLIQQHRVQQMAAVLAENTRLRQLLNSADKLEN
                     RVLVAELIGVAPDPMLHRILVNRGQVDGVYIGQPVVDAQGLVGQVVEVGENSSWVILI
                     TDTSHALPVQINRNGARLVAEGRGNLYQLGLRHVPNNFDIQEGDLLVSSGLGKRFPNG
                     YPVAVVESIKPDKSSPWAEVIAKPLAELNRNRHVLLVFEEAPQLGRGDAAAPPPAANA
                     SSSSSSSSSSSSASSSSSS"
     gene            complement(946..1977)
                     /locus_tag="PKZ52_17895"
     CDS             complement(946..1977)
                     /locus_tag="PKZ52_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNG61701.1"
                     /translation="MFKRLRGMFSNDLSIDLGTANTLIYVRDRGIVLDEPSVVAIRNI
                     NGQKSVEAVGMAAKRMLGRTPGNITAIRPLKDGVIADFQVTEKMLQHFIRKVHENSWF
                     QPSPRVLICVPCMSTEVEKRAIRESALGAGARHVDLIPEPMAAAIGAGLNVDQATGSM
                     VVDIGGGTTEIAIISLNGVVLSDSVRIGGDRFDEAIVSFVRRKYGTVIGEATAERIKK
                     EIGSAYAGSKVLEIDVRGRNLAEGVPRSFTLNSDEVLEALQEPLSGIVQAVKSALEQS
                     PPELASDIAESGIVLTGGGALLRDLDRLLMEETGLPVLVAEDPLTCVARGGGKALELM
                     DGRNFDFLS"
BASE COUNT          421 a          618 c          602 g          423 t
ORIGIN      
        1 tgctggagct agaactggag gcgctactgg agctggaact ggaactgctg ctggagcttg
       61 cattggctgc agggggcggt gccgcagcat cacccctgcc cagctggggg gcctcttcaa
      121 acaccagcaa tacgtggcgg ttgcggttga gttctgccag gggcttggcg atgacttccg
      181 cccaggggct ggatttatcc ggcttgatgg attccaccac cgccactggg taaccattcg
      241 ggaagcgctt gcccaggccc gaactcacca acaggtcgcc ctcttggata tcaaaattat
      301 taggcacatg gcgcaggccc aactggtaca aattgccgcg cccttcggcc accaagcggg
      361 cgccgttgcg gttgatctgc accggcaggg cgtggctggt gtcggtaatt aaaattaccc
      421 agctggagtt ctcccccact tccaccacct gccccaccaa accctgggcg tccaccaccg
      481 gttggccgat atacacacca tccacctggc cccggtttac caaaatacgg tgcagcatag
      541 ggtctggggc gacgccgatc agctcggcca ccagtacgcg gttttccagt ttgtcggcgg
      601 aattaagcag ctggcgcagg cgggtgttct ccgccaacac cgcggccatc tgctgcaccc
      661 tgtgttgttg gatcaaaagc tccgtgcgca gggcttcgtt ctcctgcagc agctgctctt
      721 tgggcaccag gcgctgctgg ctccaggcgc tcatctcccg gggcatgttg gccagccaat
      781 aaaagggcgt ggctatatcg atggcctttt gtttgagggg gccgagccat tgggtttggg
      841 tatacaccaa cgccaatgcc acagcggcca ccgccagcag cgccaagcga acccccgcag
      901 aggggctttt gcgaaataag ggtttaatag ctcaccccga ggggattagc ttaagaagtc
      961 gaaattgcgg ccgtccatta attccagtgc cttaccgccg ccgcgcgcta cacaggtgag
     1021 cgggtcttca gccactagaa ctggcaggcc agtctcttcc attaacaagc ggtctaaatc
     1081 ccgcaacagg gcgccgccac cggtgagcac tatgccgctt tcggcgatgt ccgaagccag
     1141 ctcaggcggg ctttgctcca gcgcgctttt caccgcctgc actatacccg agaggggctc
     1201 ttgcagggct tccagcactt catcgctatt gagggtaaag cttctgggca cgccttcggc
     1261 caggttgcgg ccgcgcacgt cgatctccaa cactttggag ccagcgtagg cgctgccgat
     1321 ttcttttttg atgcgctcgg cggtggcctc accaattacg gtgccgtatt tgcggcgcac
     1381 aaaactgacg atggcttcgt cgaagcggtc gccgccgata cgcacggaat cagacagcac
     1441 cacaccgttt aacgaaatga tggctatttc cgtggtgccg ccgcctatat ccaccaccat
     1501 ggaaccggtg gcttggtcaa cgtttaaacc tgcacctatg gcagctgcca tgggctccgg
     1561 gatcaggtcc acatggcggg caccggcgcc caaggcggat tcgcgaatcg cgcgtttttc
     1621 cacctcggta gacatacagg gcacacagat cagcactcgg gggctgggct ggaaccagga
     1681 gttttcatgc actttgcgga taaagtgctg caacattttt tcggtaactt ggaagtcggc
     1741 gatcacgcca tctttgagcg ggcgaatagc cgtgatgttg ccgggggtgc ggccgagcat
     1801 gcgcttggca gccatgccca cggcctcaac ggatttttgc ccgttgatat tgcgaatggc
     1861 caccaccgat ggctcatcca acactatgcc gcggtcgcga acgtaaatca gggtattggc
     1921 ggtgcccagg tcaatggaca gatcattaga gaacataccg cgcaggcgtt taaacatagc
     1981 gcttcaaacc tttaataaag acgggggaca aaaatagggg ggaactgcca tgaaaacggc
     2041 cgacagtagc ggcaaccgcc cgtg
//
LOCUS       DAOQGS010000697         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_36883_length_2061_cov_3.935194, whole genome shotgun sequence.
ACCESSION   DAOQGS010000697 DAOQGS010000000
VERSION     DAOQGS010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36883_length_2061_cov_3.935194"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKZ52_17900"
     CDS             <1..379
                     /locus_tag="PKZ52_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNG61702.1"
                     /translation="SYALSAGYYDAYFTKAQQARRLIQEDFVAAFKQVDLILGPTCPG
                     PAFGFGAKGKDPVAMYLEDIYTIATNLAGLPGMSIPCGLADGLPVGLQIIGNYFAEGL
                     MLNAAHQYQQATSFHTLAPANIA"
     gene            405..1844
                     /gene="gatB"
                     /locus_tag="PKZ52_17905"
     CDS             405..1844
                     /gene="gatB"
                     /locus_tag="PKZ52_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNG61703.1"
                     /translation="MPWETVIGLEVHVQLATKTKIFSGASTAFGAEPNTQACAIDLAM
                     PGTLPMPNAEAFKMAAMFGLGINAEINRRSYFERKNYFYPDLPKGYQTTQLAQPIVGA
                     GFLEIDTAAGPKKIRIHHAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLIEVVSEPDIS
                     NAEEAVAFAKKLHGLVTALGICDGEMSQGSLRFDVNISVRRPGEPLGTRTETKNLNSF
                     KFMEEAIELEVERQITLIESGGKVIQETRLYNGDTQKARSMRSKEDSNDYRYFPCPDL
                     LPVVLDDAFMDAVRAAMPELPEAKAARYQSEYGLPAYDAGILSSDRTTADYFETAAKV
                     SGQAKLAANWVMGEVAAQLNRHEISLKDCPVSAGQLGQIIKRIFDGTITSKSGKTLFD
                     AIWAGEGIDADTLIKSKGLQQMNDTGALEKIIDDIITANASQVEAYRAAPEDKKAKML
                     GFFTGQAMKASKGQANPQQLNALLLAKLN"
     gene            1876..>2061
                     /locus_tag="PKZ52_17910"
     CDS             1876..>2061
                     /locus_tag="PKZ52_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HNG61704.1"
                     /translation="MSEKKDKKKVVVETFTDERVRTFLEGTPYGDINPDYYLLERAYR
                     GMNEENFATFVGFFVEAG"
BASE COUNT          544 a          596 c          516 g          405 t
ORIGIN      
        1 cagctatgcg ctttccgccg gttactacga cgcctacttc accaaggcgc agcaggcgcg
       61 ccgcttaatt caagaggatt ttgtcgccgc ctttaaacag gtggacctga tcctcggccc
      121 cacctgcccg gggcccgcct tcggctttgg cgccaagggc aaagacccgg tggccatgta
      181 tttagaagac atctacacca tcgccactaa cttagccggc ctgcccggta tgtctatccc
      241 ctgtggcctg gccgatggcc tgcccgtcgg cctgcaaatt atcggcaact attttgccga
      301 gggcctgatg ttaaacgcgg cccaccagta ccaacaggcc acctccttcc acaccctggc
      361 cccggccaac atcgcctagg agcccaaaat cgatgaatag acatatgcca tgggaaaccg
      421 ttatcgggct ggaagtacac gttcagctcg ccaccaaaac caaaatcttt tcaggcgcaa
      481 gcaccgcctt cggcgccgag cccaacaccc aagcctgcgc cattgattta gccatgcccg
      541 gcaccctgcc tatgcccaac gccgaagcct tcaaaatggc ggccatgttt ggcttaggga
      601 taaatgcaga aattaaccgc cgctcctatt ttgaacgcaa aaattacttc tacccggacc
      661 tgcccaaggg ttatcaaacc acccaactgg cgcagccgat tgtgggcgcc gggtttttgg
      721 aaattgacac cgctgccggg ccgaaaaaaa ttcgcatcca ccacgcccac ctggaagaag
      781 acgccggtaa gtcactacat gaagacttcc acggcatgtc cggtatcgac ctcaaccgcg
      841 ccggcacacc gctcattgaa gtggtgagcg agccggatat cagcaatgcc gaagaggccg
      901 tggcgtttgc caaaaagctg cacggcttgg taacggcgct gggtatttgt gatggcgaaa
      961 tgagccaggg ctcattgcgt tttgatgtga atatttctgt gcgccgcccc ggtgagccgc
     1021 tcggcacccg caccgaaacc aaaaacctca actcgtttaa atttatggaa gaggccattg
     1081 aactggaagt tgagcggcaa attacgctga ttgaaagcgg cggcaaggta attcaggaaa
     1141 ctcgcctcta caacggcgac acccaaaaag cccgcagcat gcgctccaaa gaagactcca
     1201 acgactaccg ctacttcccc tgcccggatt tattgccggt ggtattggat gatgccttta
     1261 tggacgcggt gcgcgctgcc atgccggaac tgcccgaggc caaagccgcc cgctaccaga
     1321 gcgaatacgg cctacccgcc tacgatgcag gaatattaag tagcgaccgc accaccgccg
     1381 attattttga aaccgcggcg aaggtgagcg gccaagcaaa attagcggcc aactgggtaa
     1441 tgggtgaagt ggccgcgcaa ttaaaccgcc acgaaatcag cctaaaagac tgcccggtca
     1501 gcgccgggca gttagggcaa attattaagc gcatttttga tggcaccatc accagcaaaa
     1561 gcggtaaaac cctgttcgat gctatttggg ccggggaagg catcgacgcc gacaccttaa
     1621 ttaaatccaa aggcctgcaa caaatgaacg acacgggcgc tttggaaaaa ataatcgacg
     1681 acatcatcac cgccaatgcc agccaagtag aagcctaccg cgccgcaccc gaggataaaa
     1741 aagccaaaat gctgggcttt ttcaccggcc aagcgatgaa agcctccaaa ggccaagcca
     1801 acccccaaca attaaacgcc ctgttattag caaaattaaa ttaatagccg gccgaattaa
     1861 acataaaggt tttctatgag cgagaaaaaa gacaagaaaa aagtagtagt ggaaactttt
     1921 acggacgagc gcgtgcgcac gtttttagaa ggcaccccct atggcgatat caatcctgat
     1981 tattatttat tagagcgagc ctatcgcggt atgaatgaag agaattttgc tacgttcgtg
     2041 ggcttttttg tcgaggcggg t
//
LOCUS       DAOQGS010000698         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_36884_length_2061_cov_3.792124, whole genome shotgun sequence.
ACCESSION   DAOQGS010000698 DAOQGS010000000
VERSION     DAOQGS010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36884_length_2061_cov_3.792124"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="PKZ52_17915"
     CDS             complement(<1..1494)
                     /locus_tag="PKZ52_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNG61705.1"
                     /translation="MKTLPLLGLLLAALGAQAKSPAVQSTQGQVLAAQMPASLSINEK
                     NYFHMQGLDVTVFSDIYPDGHQTGVTIIQHGSRVAANGDVRLEISPGQWSPVPVGGSL
                     SVDKAGRSISQTMSYPDPSKDKKGFNPINYPDLKFTYKVNVTALTGSQFKISVDLDQP
                     LPPEWVGKVGFNFELFPGELFGKAFLMDQQTGIFPRQANGPIESHHGEFLAAPLATGK
                     TLTIAPDDDQRRITINNARGTLELWDGRSNHNNGWYIVRSSIPANATKNAIEWVVTPN
                     VVKDWVYSPVLQISQVGYTRLQPKKLVIEQDATDLKADEVRLYKLTAQGKQLLRKGAP
                     VAWGKFLRYHYLTYDFSDVTEAGSFVLEYRGQSTAAFKIGDDVFARHVWQPTLEYFLP
                     MQMCHMRVNEKYRVWHGLDHLDDAIMAPVNLNHYDGYISGQSTLTKFKPGERVPHLDA
                     GGWHDAGDFDLRVESQMGTVWLLAAMVEEFGLNYDATSIDQKNKLVEI"
BASE COUNT          523 a          523 c          474 g          541 t
ORIGIN      
        1 aatttccacc agcttatttt tttgatcaat actcgtagcg tcgtaattca ggccgaattc
       61 ttccaccatg gcagccaaca gccaaaccgt acccatttgg gattccaccc gcaaatcaaa
      121 atcccctgca tcatgccaac cgccagcatc taaatgcggt acgcgctcac cgggtttaaa
      181 tttcgtaaga gtggactggc cggaaatata accgtcgtaa tggttaagat ttaccggcgc
      241 catgatggcg tcatctaaat gatctaagcc gtgccatacg cggtattttt cgttcacccg
      301 catatggcac atttgcatgg gcagaaaata ttccaaggta ggctgccaca catggcgggc
      361 aaatacatca tcgccaattt taaatgcagc agttgattgc ccacggtatt ccaatacaaa
      421 acttcctgct tccgttacat cactaaaatc ataggtgagg tagtggtagc gcaaaaattt
      481 cccccaggcc acaggcgcgc ctttgcgcag taattgtttg ccttgtgcgg ttaatttata
      541 cagacgcact tcatcggctt ttaggtctgt tgcatcctgc tcaatcacta atttttttgg
      601 ctgcagccgt gtataaccca cttgggatat ttgcagcacg ggagaataaa cccagtcttt
      661 cactacattg ggcgtaacca cccattcaat ggcatttttt gtggcattgg cgggaatact
      721 cgagcgcaca atataccacc cattattgtg attactgcgg ccatcccaca gctctaaagt
      781 gccgcgtgca ttattgatgg taatgcgccg ctgatcatca tccggcgcta tggtgagggt
      841 tttgccggtg gctaagggcg ctgctaaaaa ttccccgtgg tgggattcaa tggggccgtt
      901 ggcctgacgg gggaaaatcc ccgtctgctg atccattaaa aatgctttgc cgaataattc
      961 gccggggaat aattcaaaat taaatcctac tttacccacc cactcagggg gcaagggttg
     1021 gtctaaatcc acactgattt taaattgact gccagttaag gccgtaacat tcaccttgta
     1081 ggtgaatttt aagtcagggt aattaattgg gttaaaaccc tttttgtcct tacttgggtc
     1141 cggatacgac atggtttgag aaatactgcg gcctgcttta tccaccgaca ggctgccgcc
     1201 caccggcacc ggcgaccact gccccgggga aatttccaag cgcacatcgc cattggcggc
     1261 cacgcggctg ccgtgctgaa taatcgtcac accggtttga tggccatcgg ggtaaatatc
     1321 gctaaacacc gtgacatcca gaccctgcat atgaaaataa tttttttcat taatgctgag
     1381 gcttgcaggc atttgtgccg ctaaaacctg cccctgtgta gattgtaccg ctggggattt
     1441 tgcctgcgcg ccgagggcgg ccagtaaaag ccccaaaagt ggtaatgttt tcataggcga
     1501 tataccttgc atttaaggaa ggaaattgtg gcgagcagct tacctcagga attcgcggat
     1561 aaaagcaaca gacgccgcag aatcgggggt ttatcgttat taagaaaata tttaattaag
     1621 cagaataatc cgatttacct ggcggaattt tcacagtcgc ctcgctcaat ctcgcatggt
     1681 ttgagcgctt aggttcagct gcgcacttat ttttagcact tattctcccc acactatatc
     1741 tggctaaact taggtaaacc ctgagcccct atataattcg ctcacggtgc agcagcactg
     1801 gatacaggtt atttcagaca gtcaaaaaga gggacctttt tgacgagcct acgcggaagc
     1861 attcacggat gtattcacgg cgtgtctgaa ataacctgta tccagtgctg cggctggggt
     1921 tggcaaggct cgccaatact taaggaacct cggaaaaatt accttactcg ctctggcgcc
     1981 gacatttttc agaggctcct aagtcttcgc cctatgattc cagcccgcag gggaatcacc
     2041 gctaccgagc aataccctaa a
//
LOCUS       DAOQGS010000699         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_36959_length_2059_cov_3.321357, whole genome shotgun sequence.
ACCESSION   DAOQGS010000699 DAOQGS010000000
VERSION     DAOQGS010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36959_length_2059_cov_3.321357"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKZ52_17920"
     CDS             complement(<1..478)
                     /locus_tag="PKZ52_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HNG61706.1"
                     /translation="MIKPLVLLFAAIATICAQAETDETQKQTWQFGLGLGSQTLADYR
                     GSSYYQTQFLPIPFVIYNGEHIKIDRDGARGEYRWSDQLEFNVSADAALNGDSEDNPL
                     RAGMPQLNSAFEIGPSINWAFAGSTMQNGWSLRLTLRSVYTYGSEGFGHQGYTFSPK"
     gene            complement(591..>2059)
                     /gene="pnp"
                     /locus_tag="PKZ52_17925"
     CDS             complement(591..>2059)
                     /gene="pnp"
                     /locus_tag="PKZ52_17925"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNG61707.1"
                     /translation="CNRHPRDLEALATAHAVVAGQGPLGLAAITERFLSWSKEKAARY
                     DALSKLRSEAVAQLAAEGSAFSAEQVKEAFETFEYNLVRGRIVAGEPRIDGRDNKTVR
                     GLNIEIGVLPRVHGSALFTRGETQALVAATLGSPRDAQLVEGLEGERKEGFMLHYNFP
                     PFSVGECGRMGATGRREIGHGRLARRGVAPMLPSDADFPYSIRVVSEITESNGSSSMA
                     SVCGTSLALMDAGVPMKAPVAGIAMGLVKEGDKFAVLTDILGDEDHLGDMDFKVAGTA
                     NGVTALQMDIKIQGITEEIMEIALQQALQARLHILAEMNAIIAKPRATLSSNAPQYHT
                     MKVDPDKIRDIIGKGGATIRSITEETGASIDIDDNGTVKIYASNGDGLQAATDRILAI
                     TAEAEIGAIYEGTVVRIVDFGAFVNFLPGKDGLVHISQIAHERVNAVSDYLKEGQVVK
                     VKCLDVDNRGRIKLSIKETLPAPEPTAPVAQEEDAAQE"
BASE COUNT          419 a          553 c          631 g          456 t
ORIGIN      
        1 gtttcgggct aaaggtatag ccctggtggc caaagccttc ggagccgtag gtgtacaccg
       61 aacgcagggt aaggcgcaga gaccagccgt tttgcatagt gctacctgca aaagcccaat
      121 tgatggaggg gccgatttca aaggcggaat ttaattgcgg catacctgcg cgcagggggt
      181 tgtcttcact gtcgccattt aaggccgcat cggctgaaac attaaattcc aactgatcgc
      241 tccagcggta ttcgccccgc gcgccgtcgc ggtcaatctt tatgtgctca ccgttataaa
      301 tcacaaacgg gatgggcaga aactgtgtct ggtagtagga cgagcctcgg taatccgcca
      361 gggtttggct gccaaggccc aggccaaatt gccatgtttg cttttgggtt tcatcggttt
      421 cggcctgtgc gcagattgtg gcaatggcgg cgaaaagcaa aacaaggggt ttaatcatgg
      481 tgacctgggg ctcgcaagag acagtgaaag agcgctgcaa tggaatctgc aacgaattga
      541 tttgacgcaa aaaaaaaccc gccgcagcgg gttttacagc gcgcgaagga ttattcctgg
      601 gcggcatctt cttcctgggc aactggcgct gtaggctcgg gtgctggcag ggtttctttg
      661 atggagagtt taatgcggcc gcggttatcc acgtccaaac acttaacctt caccacttgg
      721 ccttctttta ggtaatcgga aacggcattc acgcgctcgt gggcgatttg cgagatatgc
      781 accaggccgt ctttaccggg taagaagtta acaaatgcgc cgaagtctac gatgcgcact
      841 acggtgcctt cgtagatagc gccaatttcc gcttcggcgg taatggccaa aatgcggtcg
      901 gtggctgcct gcaggccgtc gccattgctg gcgtagattt ttacggtgcc gttgtcgtcg
      961 atatcgatag aggcgccggt ttcttcagta atcgagcgga tagtggcgcc gcctttaccg
     1021 atgatgtcgc ggatcttatc ggggtccact ttcatggtgt ggtattgcgg tgcgttggag
     1081 gacagggtcg cacggggttt ggcgatgatg gcgttcatct cggcgagaat atgcaggcgc
     1141 gcttgcaaag cttgctgcag ggcgatttcc ataatctctt cggtgatgcc ttggatttta
     1201 atgtccattt gcagggcggt gacaccgttg gcggtaccgg ccactttaaa gtccatatcg
     1261 ccgaggtgat cttcatcgcc gaggatgtca gtcaacacag cgaatttatc gccttctttc
     1321 accaagccca tggcaatacc ggccacaggg gctttcatgg gtacaccggc gtccatcagc
     1381 gcaaggctgg tgccacacac ggaggccatg gagctggaac cgttggattc ggtaatttcg
     1441 ctcaccacgc ggatgctgta ggggaaatcc gcatcgctgg gcagcattgg agctacgccg
     1501 cgacgggcca ggcggccgtg gccgatttca cggcggccag tggcacccat acggccgcac
     1561 tcacccaccg agaagggagg gaagttgtag tgcagcataa agccctcttt gcgctcgcct
     1621 tctaagcctt caaccagctg ggcatcgcgg ggcgagccta gggtggcggc gaccagagct
     1681 tgggtctcgc cacgagtgaa cagggcggag ccgtgcacgc ggggcagaac gccgatctcg
     1741 atattcaagc cgcgtacggt tttgttatcg cggccatcga tgcgcggttc accggcaacg
     1801 atgcggccgc gcactaggtt gtactcaaag gtttcaaaag cttctttcac ttgctcagcg
     1861 ctgaacgcgg agccttcagc ggccaactgg gctaccgctt cgctgcgcag tttagagagc
     1921 gcatcgtagc gggcggcctt ttccttggac caggacaaaa accgctcggt gatagcggcc
     1981 aggcccagtg gcccctgccc ggccaccacc gcgtgggccg tggccagcgc ctccagatcc
     2041 cgagggtgcc ggttgcaga
//
LOCUS       DAOQGS010000700         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_37159_length_2052_cov_4.317476, whole genome shotgun sequence.
ACCESSION   DAOQGS010000700 DAOQGS010000000
VERSION     DAOQGS010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37159_length_2052_cov_4.317476"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKZ52_17930"
     CDS             complement(<1..610)
                     /locus_tag="PKZ52_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNG61708.1"
                     /translation="MLASKTRALLVSRAFRLAIGVLIILSMVSFSFDTVPDLDPTLKR
                     LLDYFECFTIGVFTLEYFARITTAQQKLKFVFSFYGLIDLVAILPFYLMLMVDLRSLR
                     ALQLLRLLRVLKMGRYGGAMDRFLAAIRQTKEELLIFSFIALILLYLSAMGIYYFEHA
                     AQPDKFRSLFDCIWWAAVTLTTVGYGDIYPITIGGRIFTVFIL"
     gene            complement(613..>2052)
                     /locus_tag="PKZ52_17935"
     CDS             complement(613..>2052)
                     /locus_tag="PKZ52_17935"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNG61709.1"
                     /translation="GLIAGATGTGKTVSLQSLAEGFSRAGVPVFMADIKGDLSGLAAA
                     GAASEKLAAACEKRHLTLPNFAANSCVFWDLFAAQGHPIRATISDMGPLMLARLFNLN
                     EVQTGVLQLVFKVADDQNLLLLDIKDLRAMLRYVGENSKTLAAEYGNISAASIGAITR
                     ALLTVEEEGGGAFFGEPMLNILDLIQTDAEGMGVINILAGERLMQQPRIYACFLLWLM
                     AELFEQLPEVGDCAKPKIVFFFDEAHLLFNDAPQALLEKIEQVVRLIRSKGVGIYFVT
                     QNPLDIPDTVLGQLGNRIQHALRAFSPKDQKAVKAAAQTLRANPKVDTATAITELAVG
                     EALVSLLDSEGRPAPVERAYIIPPGSRLGPLSAQERTTAVRQSPLAGIYERTEDSVSA
                     YEQLTQNSAPAAEPSKGPNADSASAEGGLGGVMQDVIFGSTGPRGGKREGLVETAAKT
                     LVRQLASEVGRQLVRGILGSLSGSPRRRR"
BASE COUNT          540 a          544 c          513 g          455 t
ORIGIN      
        1 acagaataaa aacggtaaaa atgcgcccgc cgatggtgat ggggtaaata tcgccataac
       61 ctacagtcgt cagggttacc gccgcccacc aaatacaatc aaacagcgag cgaaatttat
      121 ctggctgcgc ggcgtgttca aagtaataaa ttcccatggc ggataaatac aggagtatta
      181 gtgcaataaa actaaaaatt aataattcct ccttggtttg gcgaatggcg gcgaggaatc
      241 gatccatagc gccgccataa cggcccattt ttaacacccg caataaacgc agtaattgca
      301 aggcgcgcag ggagcgcaag tccaccatca acattaagta aaacggcaga atcgctacca
      361 gatcaatcag gccgtaaaaa ctaaacacga atttaagctt ttgctgcgca gttgttatgc
      421 gggcaaaata ttcaagcgta aacacaccga tggtaaaaca ctcgaagtaa tccaacagcc
      481 gcttaagggt aggatctaaa tcgggcactg tatcgaagga aaaactcacc atcgataaaa
      541 taattagcac cccaatggct aaacgaaaag cgcgcgatac cagcagcgcg cgggttttac
      601 tggccagcat ttttagcggc gcctgcgcgg gctacccgag agggaaccta atatgccgcg
      661 caccaattgc cgccccactt cacttgcgag ctggcggacg agcgtttttg ccgctgtttc
      721 aactaacccc tcccgtttgc cgcccctggg gccggtactg ccgaaaatga catcctgcat
      781 tacacctcct aaaccgcctt cggctgacgc tgaatcagca ttggggcctt tggaaggttc
      841 agctgccggg gcagaatttt gggttaattg ctcataggcg gagacgctgt cctcggtgcg
      901 ctcataaata ccggccaagg gagattgccg tacggcggta gtgcgttcct gcgcggacag
      961 ggggcctaag cgggagccag gtggaattat gtaggcgcgc tcaaccggtg cagggcgccc
     1021 ttcactatcc agcagggaaa ccagcgcctc acctacagcc agttcagtga tggccgttgc
     1081 ggtatccact ttggggttag ccctgagggt ttgggcagcc gcttttaccg ctttttgatc
     1141 tttagggctg aaggcgcgca gggcgtgttg tatgcgatta cccaactgcc ccaatacggt
     1201 gtcgggaatg tccaaagggt tttgcgtgac aaaatagatt cccacaccct tagagcgaat
     1261 tagccgcacc acctgttcaa ttttttccaa taaggcttgc ggggcatcgt taaacaacaa
     1321 atgggcttca tcgaagaaaa aaactatttt gggttttgcg caatcgccca cttcaggcaa
     1381 ttgctcaaat aattcagcca ttaaccagag taaaaaacag gcgtagattc gcggctgttg
     1441 cattaagcgc tcccccgcca ggatatttat cacgcccata ccctctgcat cggtttgaat
     1501 caggtcgaga atatttaaca tgggctcgcc aaaaaaagcc cctccgccct cttcttccac
     1561 cgttaataat gcgcgggtga tagcgcctat tgaggccgct gagatattgc cgtattcggc
     1621 ggcgagggtt ttgctattct cgcccacata acgcagcatg gcgcgcaggt ctttaatatc
     1681 cagcaataat aaattctggt catcagcgac tttaaacacc aattgcagta cgcctgtttg
     1741 cacctcgttt aaattaaaca gccgcgccaa cattaatggc cccatgtcgg aaatagtcgc
     1801 ccgaatgggg tggccttggg cggcaaataa atcccaaaaa acacagctat tggcagcaaa
     1861 attaggcagc gttaggtggc gtttttcaca ggctgcggcg agtttttcac tggctgcgcc
     1921 cgccgccgca aggccggata aatcgccttt aatatcggcc ataaagacgg gcacacccgc
     1981 gcgggagaat ccttccgcca acgattgcaa actgactgtt ttgccagtgc cggtagcccc
     2041 ggcaataagg cc
//
LOCUS       DAOQGS010000701         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_37487_length_2042_cov_4.883744, whole genome shotgun sequence.
ACCESSION   DAOQGS010000701 DAOQGS010000000
VERSION     DAOQGS010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37487_length_2042_cov_4.883744"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1357
                     /gene="rimO"
                     /locus_tag="PKZ52_17940"
     CDS             20..1357
                     /gene="rimO"
                     /locus_tag="PKZ52_17940"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNG61710.1"
                     /translation="MSNSSPTVGFVSLGCPKALVDSERILTQLRTEGYQLVPSYEGAD
                     VVVVNTCGFIDSAKQESLDAIGEAIKENGRVIVTGCMGVEEKTIRDLHPSVLAVTGPQ
                     QYEQVVNAVHQVVPPKQNHDPFIDLVPPQGIKLTPRHYAYLKISEGCNHRCSFCIIPS
                     MRGDLVSRPIGEVLDEAERLVKAGVRELLVISQDTSAYGVDAKYRTGFWQGRPVKTRM
                     LELCDALSELGVWTRLHYVYPYPHVDEVIPLMAAGKILPYLDIPLQHASPSILKAMKR
                     PAHQEKTLDRIHAWRKQCPDITLRSTFIVGFPGETEADFQFLLDWLDEAQLDRVGCFE
                     YSPVEGAPANELPGAVPDEVKRERWERFMEKQQAISEAKLAAKIGRTIEVLVDEVHED
                     GAIARSSADAPEIDGVVHIEGDYGLRQGDMLMVQVEDADEYDLFAVPADVEEE"
BASE COUNT          422 a          527 c          598 g          495 t
ORIGIN      
        1 tttttttagc gggtcgttta tgtctaattc ctcacctacc gtagggtttg tttctctcgg
       61 ttgtcctaag gcgctggtgg attccgagcg cattttgacg cagctgcgca cggaaggtta
      121 tcagctggtg cccagttacg aaggcgccga tgtggtggtg gtaaacacct gcggctttat
      181 tgatagcgcc aaacaggaat ccctggacgc cataggcgag gccatcaaag agaacggccg
      241 ggtgattgtg accggctgta tgggggtaga ggaaaaaacc atccgcgatt tacaccccag
      301 tgtgctcgcc gttaccggcc cccagcagta cgaacaagtg gtgaacgcgg tgcaccaagt
      361 ggtgccgccc aagcaaaacc acgatccctt tatcgacctg gtgccgcccc agggcattaa
      421 attaaccccc cgccattacg cctatttaaa aatatccgaa ggctgtaacc accgctgtag
      481 tttttgcatt attccctcca tgcgcggcga cctggtgagc cgcccgattg gggaggtatt
      541 agatgaggct gagcgcctgg tgaaggccgg ggtgcgggaa ttattggtga tctcccaaga
      601 caccagcgcc tatggtgtgg atgccaaata ccgcaccggc ttttggcagg gccggccagt
      661 gaaaacccgt atgttggaat tgtgcgatgc cttaagtgag ctgggcgtgt ggactcgcct
      721 gcactatgta tatccctacc cccatgtgga tgaggtgatc cccctgatgg cggcgggcaa
      781 aattctgccc tacttggata ttcccctgca gcacgccagc ccctcgattt taaaggccat
      841 gaagcgcccg gcccatcagg aaaaaaccct cgaccgcatc cacgcctggc gcaaacagtg
      901 cccggacatc accctgcgct ccacctttat tgtgggcttt cccggcgaga ctgaggcgga
      961 ttttcagttt ttgctggact ggctggatga agcgcagttg gatcgcgtgg gctgttttga
     1021 atattcaccc gtggagggcg cccccgccaa cgagctgccc ggcgccgtgc cggatgaagt
     1081 taagcgcgag cgctgggagc ggtttatgga gaaacaacag gcgatttccg aagccaagct
     1141 cgccgccaaa attggccgta ctattgaggt cttggtggat gaggtgcacg aggacggcgc
     1201 catcgctcgc tccagcgccg atgcgccgga aattgacggc gttgtgcata tagaaggtga
     1261 ttacggcctg cgccaggggg atatgttaat ggtgcaggta gaagatgcgg atgaatatga
     1321 cctgtttgcg gtgcctgcgg atgtggaaga ggagtagtgg gctttagagg gggtatttct
     1381 gccacatttt gggcggcttt tgagtgttat tgcttcctta aatgctcttc ctatgggggc
     1441 actttcattg agttaagagt tggctaacct ttttcgccat gctttgggcc taaaattccc
     1501 ctcctgtgga ggggtgaccc gtaggggtgg ggtggtaggt cgttgcgctg tttttttgtt
     1561 cgcagcctgc cgccctacag ataagagatt gcctagttcc cttaaaaaac acagactccg
     1621 tcaccgcaac cacccgcctg ccacccctcc acaggagggg aattttaggc cgtgcctgca
     1681 gataaaacga actcttaatt aacgatgtag ttttttgtgg cactgaactc tgcgagccgt
     1741 ttattcatgg ctcttctggt tggcttctct gttcagtcat tagccgtcgg cggttattga
     1801 gcccgaacct gttttcaatc ccgccttggc tgcatctgtg accaattcgg cgaaattgat
     1861 ttggctggtc tatgctcaag caaggtgcta gcaagagaaa gactgactcc atggcaaaat
     1921 taacggacgc ttgtgaacca ggctcagggg atgcggtggc ctcggcaatt cgggccctca
     1981 tgccgcctga ggccagtgag gaggtgctaa acgattgtct gcgcctattg ttggagcaga
     2041 tg
//
LOCUS       DAOQGS010000702         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_37701_length_2035_cov_3.966667, whole genome shotgun sequence.
ACCESSION   DAOQGS010000702 DAOQGS010000000
VERSION     DAOQGS010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37701_length_2035_cov_3.966667"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..978)
                     /locus_tag="PKZ52_17945"
     CDS             complement(376..978)
                     /locus_tag="PKZ52_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNG61711.1"
                     /translation="MRIHYSITSPFARKVRIFAALTQEPNIQWVLTKPMETPDFRSIN
                     PLGKIPALQTDAITLFDSALICEYLDELHCARGGASVLHRGQADYFIVQKNHYLANGI
                     MEAAVASVMDQRRPDASASAFWLKRWQDSIEAGLKVAAVDALGTKGNLNIGGIAMVCA
                     LGYLALRLPELNPAAINPELAAWYAAQCQEPWFLATVPQD"
     gene            1133..1627
                     /locus_tag="PKZ52_17950"
     CDS             1133..1627
                     /locus_tag="PKZ52_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNG61712.1"
                     /translation="MGRYLFKLGVFLSLIAGIGNYVVYLGTGRVPFNDLWQHFKKTPV
                     EIPKPGTLDLPSLPKLPSLGGKQESRAYKWMDKNGVVHYADQPPDGQNAQLINVDPDQ
                     NLVQGSPPPVQESAPAKTKTTPSQAASNPVLNGSPAMDNASMQQLLQQIKAAAESAKQ
                     RPIE"
BASE COUNT          564 a          521 c          485 g          465 t
ORIGIN      
        1 aaagataagg attcaaacat actcaaacct aaatgattaa tttaaaaatt gtgctaaaaa
       61 atccgtgcag cgctaactca ggttggataa acaactgacg taaacgcgat tacctacata
      121 taagcaacct caagcattta taaggttgtt aacaggcaaa tctaaaaacc tccgttaaaa
      181 ctcactgtaa atgccaaagg ttccgctaag gtttttaagt tacgaatatt aatgcaagcg
      241 ctacgggcca ttcgtttatt aacttaatca gccaaaggcc gcgtttagtt aaatgccctt
      301 cggcatcttc aataaagaac ctatgtgtta ttttttcagc gccgctcaaa ggcgccgcga
      361 taaataaagg ggcagttaat cctgcggcac agtggcgagg aaccaaggct cttggcattg
      421 ggctgcgtac cacgcggcca attcagggtt gatcgccgct gggttcagct ctggcaggcg
      481 cagcgccaag taccccaggg cacagaccat ggcaatgccg ccgatattga ggttgccttt
      541 ggtgcccaag gcatcaactg cggcgacttt gaggcccgcc tcgatagaat cctgccagcg
      601 cttgagccaa aacgcagagg cgctcgcatc cgggcggcgc tgatccataa cactcgccac
      661 cgcagcctcc ataattccgt tggccaagta atggtttttc tgcacgataa aataatcggc
      721 ctggccgcga tgcaacacac tcgcaccgcc ccgcgcacag tgcagctcat cgagatactc
      781 acaaattaat gcactatcga acagggtgat ggcgtctgtt tgcaaggcgg ggattttacc
      841 caaggggttg atactgcgaa aatcgggggt ctccatgggt ttggtcaata cccactggat
      901 gttaggttct tgagttaagg cggcaaagat gcgcaccttg cgggcaaagg gtgaggttat
      961 actgtagtga atgcgcatgg cggctcccag taaacaaaag ggggcttttt accctgctca
     1021 agtggaaccc acaagggttt tcttagatac ctctgagtta taggcgctgc aaactgcagg
     1081 tagccaagca accagagccc cgcgaatttt aatccagttg gcgcacgcgt ttatgggacg
     1141 ttatttattt aaattagggg ttttcttaag tttaatcgcc ggcatcggca attatgtggt
     1201 gtatctgggc accggccgag tgccgtttaa cgatttatgg cagcatttta aaaaaactcc
     1261 cgtcgaaatt cccaagccag gcacattaga tttaccgagc ctgcctaagc tgcccagcct
     1321 aggtggtaag caagaatcta gggcttataa gtggatggat aaaaatggtg tagtgcatta
     1381 cgccgatcag ccgccagacg gccaaaatgc ccaattaatt aatgtcgatc ccgatcaaaa
     1441 tttagtgcag ggctcaccgc cgcccgtgca ggaaagcgcg cccgccaaaa ctaaaaccac
     1501 cccatctcag gctgccagca acccggtatt aaatggcagc cccgctatgg ataacgcatc
     1561 aatgcagcag ttattacaac aaattaaagc cgctgccgaa tccgccaaac aaaggcccat
     1621 tgaatagttt taacctcgaa taaaccgccc gcacaataaa aaccccagct gctccggcat
     1681 taagagcagc tagggtttta aattcaaggg cgcttattta cccggcggca attagtgccg
     1741 ggttaataaa ggtggcaagg atgccgcgtc aaaagtgcaa accgccgcgt gctgcactaa
     1801 aacaagaaaa tctgcgcatt ttcgcaagta tcagtgcagc gaaggcaaaa tagatcagga
     1861 cgatccattt tgccgccgtg ttaatagtca ttaaaaacca ctgcacaaag ccactttcgc
     1921 acaatcgctg ggtgttatag cgtttccact cgatccacct gctggaagcc gcgcggcaat
     1981 ttattaccct tgcgcccgcg ctcgcccata tagtgctcca tatccttcag gctga
//
LOCUS       DAOQGS010000703         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_38043_length_2024_cov_4.619096, whole genome shotgun sequence.
ACCESSION   DAOQGS010000703 DAOQGS010000000
VERSION     DAOQGS010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38043_length_2024_cov_4.619096"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKZ52_17955"
     CDS             complement(<1..312)
                     /locus_tag="PKZ52_17955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNG61713.1"
                     /translation="MNTTDKILTRSLLSLLISQSAFAAEKPREIEEIIVTATKRATNL
                     QDTPLAVSAFSQETLAENHVVNLNDLQGMVPSLHIAQNGTQNTPMVYMRGVGSSDQTE
                     SG"
     gene            767..1726
                     /gene="pip"
                     /locus_tag="PKZ52_17960"
     CDS             767..1726
                     /gene="pip"
                     /locus_tag="PKZ52_17960"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNG61714.1"
                     /translation="MKTLYAELRVDATGELAVDDVHRLYWEVSGNPDGVPVLFIHGGP
                     GAGSSHNDRRFFDPARYRIVVFDQRGSGRSTPHAELHNNTTAHLISDIEALREHLGID
                     RWVLFGGSWGSTLALLYAQAFPERVMAMILRGIFLCRQEDLHWFYQAGADRIFPDYWE
                     DFIAHIPEAERGNLMQAYYERLTGANELAKMSCAKAWSTWEGRCATLRPNPEIVHFFA
                     EPHRALSLARIEAHYFVNNAFIAPNQIIAHADKLKDIPGIIVHGRYDVVCPLDNAFAL
                     HQAWPGSELQIIRDAGHSSMEPSIVDALVRATDQIADDLYPAA"
     gene            complement(1756..>2024)
                     /locus_tag="PKZ52_17965"
     CDS             complement(1756..>2024)
                     /locus_tag="PKZ52_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG61715.1"
                     /translation="LIVVSVSDDGPGVPSSEQARVFESFVRLSNPQNSSQRGFGLGLA
                     IVKRLMRWHGGDVSLSAAAAGGACFTLHWPRGNGLADAAEQPQS"
BASE COUNT          423 a          522 c          559 g          520 t
ORIGIN      
        1 gccgctttcg gtttgatccg atgaacctac gccgcgcata tataccatag gagtattttg
       61 cgtgccgttt tgtgcaatat gtaaactcgg caccatgcct tgtaaatcat ttaaattaac
      121 cacgtgattt tccgccagtg tttcttggct aaatgcggaa acggctaaag gcgtatcttg
      181 caaattcgtc gcgcgttttg tcgccgtgac aattatttct tcaatttcac ggggtttttc
      241 tgcggcgaag gcactttggc tgattaataa gcttaatagg ctgcgtgtga gtattttgtc
      301 ggtggtattc acttgagtga tcccgttaac tgtgttaatc gaagcgcgca ttcaatgcgc
      361 gcgttttaaa ggcggcgcat agtaacaaaa taggcattta ttatgttacc cattcggcgc
      421 taaagaattg tgcgcctgtg cggctattga taaataggtg tgatgttatg cagaagaaat
      481 tgccaataat aaaaccggcg ctgaaggcga tattggcaag cgggcccgca cactccaggg
      541 actaatcctt ggtatttggg ccttagttat tgggatctgt ctattgggat ctagttattg
      601 cgcctctgtt gtatgcaggc tgggcgggcg agccttaggt gagatgtacg ccctgccatt
      661 cgtctaagcc cttaacagct tctattattg atgcagcgcc tactgcctat tggggggctg
      721 tggtagggtg gctgctaatc acttaacgct tttggagatg agacgaatga aaacactcta
      781 tgctgagttg cgtgtggatg ctaccggtga actggcggtg gatgatgtac accgacttta
      841 ctgggaggtc agcggcaacc cggatggtgt gccggtgtta tttattcacg gcggccctgg
      901 ggcgggcagc agccacaacg accggcgctt tttcgacccg gcccgctacc gcattgttgt
      961 gtttgatcag cgcggcagcg gccgctccac cccccacgca gagctgcaca acaacaccac
     1021 ggcccattta attagcgata tagaggcctt aagggagcat ttaggcatag atcgatgggt
     1081 gttatttggc ggctcctggg gttccaccct ggccctgctg tacgcccagg cctttcccga
     1141 gcgggtcatg gcgatgatac tgcgtggcat atttttatgt cggcaagagg atttacactg
     1201 gttttatcag gcgggtgccg accgtatttt cccggattat tgggaggatt ttatcgccca
     1261 tatccccgag gcggagcggg gtaatttaat gcaggcctat tacgagcgct taaccggcgc
     1321 caatgagctg gcaaaaatgt cctgcgccaa ggcctggtcc acctgggagg gccgctgcgc
     1381 cactctgcgg cccaaccctg aaattgtgca tttttttgcc gagccccata gggcattatc
     1441 ccttgcgcgc attgaggccc attattttgt caataacgcc tttattgccc ccaaccaaat
     1501 tatcgcccat gccgataaat taaaggatat tcccggcatt attgtgcacg gccgctacga
     1561 tgtggtgtgc cccctggata acgcctttgc gttacatcaa gcctggcctg ggtccgaatt
     1621 acaaattatt cgcgacgcgg gccattcctc catggagccg agtatcgtag atgccctggt
     1681 gcgggccact gatcagattg ccgacgacct gtatccggcc gcttaagacg gcttaagggg
     1741 gcgaacttat catttctagc tctgcggctg ttcggcggca tcggcaagcc cattgcccct
     1801 cggccagtgc agggtgaaac aggcgccgcc agcggctgcg gcgctgaggc tgacatcacc
     1861 gccgtgccag cgcatcaggc gtttcacaat cgccagaccc aagccaaagc cgcgctggct
     1921 ggagttttgc ggattgctca ggcgcacaaa ggattcaaat acccgcgcct gttcgctgct
     1981 gggcacaccg gggccgtcgt cgctgacgct gacaacaatt aatt
//
LOCUS       DAOQGS010000704         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_38219_length_2018_cov_4.478349, whole genome shotgun sequence.
ACCESSION   DAOQGS010000704 DAOQGS010000000
VERSION     DAOQGS010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38219_length_2018_cov_4.478349"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..668)
                     /gene="cobO"
                     /locus_tag="PKZ52_17970"
     CDS             complement(66..668)
                     /gene="cobO"
                     /locus_tag="PKZ52_17970"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNG61716.1"
                     /translation="MTEHQDKDHQAAMQATQEKVRAAVAAAKEKRGVIIYLYGQGKGK
                     SSSGFGTLLRAVGHGQKAAVVQFIKGTWKTGEQQFFQQHPNVQYEIMGTGFTWDSQNR
                     AKDMAAAAEVWEKAKLFLQDESINLILLDEITYMFDYGYLDLAECLSAFKQKPLQQNI
                     ILTGRSAIPELIELADTVSEINDIKHAFRAGVKAQRGIEF"
     gene            complement(720..>2018)
                     /locus_tag="PKZ52_17975"
     CDS             complement(720..>2018)
                     /locus_tag="PKZ52_17975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNG61717.1"
                     /translation="GFNARSDDKSADNDGFRAANIHIKARAQLNDAQSIGVVARSTQS
                     ANEYDGCYDSQYQRLDSCDDSAEANAARLNWRYSGTQLQQEFSLAHYINQHDRYLSSA
                     AAKNESVRGTSSDAQYFAHYAVNQAVGVSLGLAAKQETFLQKITTDNYHADEQRDSYG
                     VFSEWLIHPSEQFSYSIGGRWDSSADFGNHSSYRLASAYIIPTTGPELKWRAAASSGF
                     RSPSFYEIYFNNHSGYASPSAQKDFHDETSRGLETGLDAHWQTLSLSATVFSNKIQDE
                     IFYDALNYSGYVQTEGESQSEGVEFSGNWQMSAQVQWRLGYTHLNTEQNSSYLAGTSA
                     KTAKVQRPENTYTLGVHVELLAARWISDLNYRSAFNQRAYGGGLMEDYAVVDAQTAWQ
                     FNSSARIYLRLANLLEENYQEVKGYNTDPLSAYAGLHWSF"
BASE COUNT          450 a          510 c          513 g          545 t
ORIGIN      
        1 gccaaattcg cctagccagc gcttcgccgc ctacattttt cagaggttcc ttaatgcatt
       61 ggcggttaaa attcgatgcc ccgttgggct ttaaccccgg cgcgaaaggc gtgtttaata
      121 tcgttaattt cactcaccgt atcggcaagt tcaattaatt caggaatggc gctgcggccg
      181 gtgaggataa tattttgctg cagaggtttt tgtttgaagg cgctcaaaca ttcggccaga
      241 tccaaataac cgtaatcaaa catgtaggtt atttcgtcca ataaaataag gtttatggat
      301 tcatcctgca aaaataattt ggctttttcc cacacttcgg cggccgccgc catatctttt
      361 gcgcggtttt ggctatccca ggtaaaaccc gtgcccataa tttcatactg cacattgggg
      421 tgctgctgga aaaactgctg ctcaccggtt ttccaagtgc ccttaataaa ttgcaccaca
      481 gcggcttttt gcccgtggcc cacggcccgc agcagggtgc caaagccgga gctgcttttg
      541 cccttgccct ggccgtataa ataaataatt acgccgcgtt tttctttggc ggcggccacg
      601 gcggcgcgca ctttttcctg ggtggcctgc atggccgctt gatggtcttt atcctgatgc
      661 tcagtcataa attcaagtcc aaaaaaaggc gccattggcg cctatcaacg agtcaacaat
      721 taaaacgacc aatgcaggcc tgcatacgcg cttaaagggt cggtattata gcctttaact
      781 tcctgataat tctcctccaa tagattggct agacgtaaat aaatacgcgc ggagctgtta
      841 aattgccagg cagtttgtgc gtctactacg gcataatctt ccatcaggcc gccgccgtag
      901 gcccgctggt taaaagcact gcggtaatta aggtcgctaa tccagcgggc cgctaataat
      961 tccacatgta cgcccagggt ataggtattt tccggtcgct gcactttggc ggttttggcg
     1021 ctggtgccag ctaaataact ggagttttgc tcggtgttta gatgggtgta ccccaggcgc
     1081 cattgcactt gggcagacat ttgccagttg cccgaaaatt ccaccccttc agattggctc
     1141 tcgccctcgg tttgcacata accggagtaa ttcagggcat cgtaaaaaat ttcatcctga
     1201 attttattgc taaaaacagt ggcgctcagg ctgagggttt gccagtgggc atccaggccc
     1261 gtctctaggc cccggctggt ttcatcgtga aaatcttttt gcgcgctggg gcttgcatag
     1321 ccgctgtgat tattaaaata aatttcataa aaactcggcg agcgaaagcc gctgctggcg
     1381 gcggcgcgcc attttagttc tggcccggtg gtgggaataa tataggcgct ggctaagcgg
     1441 taactgctgt ggttgccaaa atcggcgctg ctgtcccagc ggccgccgat gctataggaa
     1501 aactgctcgc taggatgaat taaccactcg gaaaataccc cgtaagaatc ccgctgctca
     1561 tcggcgtggt aattatcggt ggttattttt tgtaaaaatg tttcctgctt ggccgcaagc
     1621 cccaagctga cacccactgc ttggttaacg gcataatggg caaaatactg tgcatcgctg
     1681 ctggtgccgc gcacggattc atttttggcg gcagcggagc ttaaatagcg gtcgtgctga
     1741 ttaatataat gggcgaggga aaattcctgt tgtagttgtg tgccgctgta gcgccaattt
     1801 aaccgcgcgg cattggcctc ggcgctgtca tcgcaactgt ccagccgctg gtattggcta
     1861 tcgtaacagc cgtcgtattc attggcgctc tgggttgagc gcgctactac acctatagat
     1921 tgcgcatcat ttaattgtgc gcgggctttg atgtggatat tcgccgcgcg aaagccatcg
     1981 ttatcagcgc ttttatcatc gctgcgggca ttaaaacc
//
LOCUS       DAOQGS010000705         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_38425_length_2012_cov_3.429228, whole genome shotgun sequence.
ACCESSION   DAOQGS010000705 DAOQGS010000000
VERSION     DAOQGS010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38425_length_2012_cov_3.429228"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKZ52_17980"
     CDS             <1..278
                     /locus_tag="PKZ52_17980"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNG61718.1"
                     /translation="WHKIRERNEALDCYVYARAAAAAAGLDRFEERHWDELERQLGSV
                     HATPMPRIRPPLQPEAVANPAPETPVVTRPQRRRVIKSRWLNLPLYH"
     gene            297..791
                     /locus_tag="PKZ52_17985"
     CDS             297..791
                     /locus_tag="PKZ52_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61719.1"
                     /translation="MSLAARIESLVLRVAQEFNDVRTKTGTITSLTTTDKTSLVAAIN
                     ELKAAVSAATDINDAQVATTSTYSSSKVVGLLNTLKSEILGGADAAYDTLVEIQQLLQ
                     NGTSGLDALLAAVNNRVRFDGAQVLTVPQQVQARANIGALAASDIGNPETDFVAIFTG
                     ALTP"
     gene            788..1177
                     /locus_tag="PKZ52_17990"
     CDS             788..1177
                     /locus_tag="PKZ52_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61720.1"
                     /translation="MSLVTRISALAQRIAQEVANKIAADHLGLARAWVSFGYANNQVS
                     IKASLNVATITRLAAGRYRVTFQSALPDANYCWVGFARSNASSGNQRFALIRPSEAKT
                     AQFLEIACATSSAGLDDSTEINLVVYR"
     gene            1177..1398
                     /locus_tag="PKZ52_17995"
     CDS             1177..1398
                     /locus_tag="PKZ52_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61721.1"
                     /translation="MAYTYEQLDALQTALAKGEHRVTFADKTVEYRSVAELKEAIALV
                     KRDLLEQAIATGLWPAPVRQIRITTVKGL"
     gene            1491..>2012
                     /locus_tag="PKZ52_18000"
     CDS             1491..>2012
                     /locus_tag="PKZ52_18000"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNG61722.1"
                     /translation="MAWRVGNPGAVAALAHSQSELRAKSRDLVRRNAWAAAGVEAFVA
                     NAIGTGIKPQSLLQDDGQRQDIHTLWNSWCEEADAAGLTDFYGLQALACRAMLEGGEV
                     LCRLRYRRPEDGLAVGLQLQLLEPEHLPHTLNQDLPSGNQIRAGIEFDRLGRRVAYHL
                     YRSHPNDGSLGAMS"
BASE COUNT          409 a          612 c          607 g          384 t
ORIGIN      
        1 agtggcacaa gatccgcgag cgcaacgagg cgctggactg ttatgtgtat gcccgagcgg
       61 cggcggccgc cgccggtctg gatcgctttg aggagcgcca ttgggatgaa ctggagcggc
      121 agctcggttc ggttcacgcc acaccgatgc cccgtatccg tccacccctt caacccgagg
      181 ccgtggcgaa cccagcgccc gagacacccg tcgttacaag gccccagcgt cggcgggtga
      241 tcaaaagccg ttggctgaat ttaccccttt atcattgagt tacgtggaga ctgcctatga
      301 gtttagctgc gcgcattgaa agcctagtgc tgcgggttgc ccaggaattt aacgacgtcc
      361 gcaccaagac gggcacgatc acaagcctca ccaccaccga taaaacctcc ttggtggcgg
      421 ccatcaatga actcaaagcg gccgtgagcg cggccaccga tatcaacgat gcgcaggtag
      481 caaccaccag cacctactcc tcaagcaaag tcgtcggcct gctgaatacg ctcaaatccg
      541 agattttagg cggtgctgat gctgcctatg acaccttggt ggagatccaa cagctactgc
      601 aaaacggcac cagtggccta gatgcgttgc tggcggccgt gaataaccgc gtgcgctttg
      661 acggcgcgca ggtgctgacc gtcccacaac aggtgcaggc gcgtgccaac atcggtgccc
      721 tggccgccag cgatatcggc aacccggaga ccgactttgt cgccattttt accggtgcat
      781 tgaccccatg agtctggtca cgcgcatcag tgcgctggcc cagcgcattg ctcaggaggt
      841 ggccaacaaa attgccgccg atcatcttgg cttggcgcgc gcctgggtga gttttgggta
      901 cgccaataat caggtcagta ttaaggccag cttgaatgtg gccaccatca cgcgcttagc
      961 ggccggtcgc taccgtgtga cctttcaaag cgctctaccg gacgccaact actgctgggt
     1021 tggcttcgcc cgcagtaacg cgagcagcgg taaccagcgc tttgctctaa ttaggccgag
     1081 cgaggccaaa accgcccagt ttctggagat cgcctgcgca accagctcgg cgggcctcga
     1141 cgactcaacg gaaatcaacc tggtggtgta ccgctaatgg cgtacaccta tgagcagctg
     1201 gatgccttgc aaacggccct ggccaaaggc gaacaccgcg ttaccttcgc ggataaaact
     1261 gtggaatacc gctctgtggc tgaattaaaa gaggcgattg cgctggtaaa acgcgatctg
     1321 ctggaacagg caattgctac cggtttatgg ccagcccctg tgcgccaaat tcgcatcacg
     1381 accgttaagg gtctttaggg attttaatga gctggttggg cgggttaacg cgtcgtctgc
     1441 tgggccagac ggccccgacc tatgatggag tgggcggcgg acgcagagcc ttggcctggc
     1501 gcgtcggcaa tcccggcgcg gtggcggcgc tggcccattc ccaaagtgag cttcgcgcca
     1561 aaagtcgcga tctggtgcgc cgcaacgcct gggcggcagc gggggttgaa gcctttgtgg
     1621 ccaacgccat tggcaccggg attaaacccc agtcactgct gcaggatgac ggccagcgcc
     1681 aagacatcca tacactctgg aacagctggt gtgaggaggc cgatgcggcg ggcctgacgg
     1741 atttttacgg cctccaagcc ctggcctgcc gcgccatgct cgaaggcggt gaagtgctct
     1801 gccgtctgcg ctaccgacgg cccgaagacg ggttggcggt ggggctgcag ctgcagttgc
     1861 tcgaacccga gcacctgccc cataccctga atcaggacct gccatcgggc aatcaaattc
     1921 gggcgggcat cgagtttgat cgcttgggcc gcagggttgc ctaccacctc tatcgatccc
     1981 atccgaacga cggaagtctc ggcgccatgt cc
//
LOCUS       DAOQGS010000706         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_38443_length_2011_cov_4.905419, whole genome shotgun sequence.
ACCESSION   DAOQGS010000706 DAOQGS010000000
VERSION     DAOQGS010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38443_length_2011_cov_4.905419"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1274)
                     /locus_tag="PKZ52_18005"
     CDS             complement(246..1274)
                     /locus_tag="PKZ52_18005"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNG61723.1"
                     /translation="MHKTLLAVGITLLSATMVGCGGGGDVTSSSSSSSSNSSSSSSGR
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSTGGLTNFQAMTTSFTEGGAIPAEFTCEGKN
                     FGDGISPEIHWTDGPTGTMSYAVAFKDMTIINSANPTNGYHWLMWNIPESINMVPKNL
                     STPAKPTEMGGATQKSAFNGGTKFFGPCPSTSCNARKKDTYSFTVYALGSLSVTPTGT
                     TVQQQIQWLEQNALKKTEVTGTSDAAPKGTNCPGSSSSSSSSSSSSSSSSSSSSSSGG
                     IDGKALFVSNCGSCHKGNGQGSNTGHDVTNHTAAEIKTAIQNVTTMRRFSTLTDAQIN
                     AIAAFLKK"
BASE COUNT          478 a          414 c          528 g          591 t
ORIGIN      
        1 tccctgcagt cgtccgcttt ttttggcaaa ttcgcctagc cagcgcttcg ccgccgacat
       61 ttttcagagg ttcccaagca ctcgggtaag gggcgttatt gctggaggcg cgcagcttag
      121 gtgtgcggat ggttggtgtg tggattttct tctaatccaa acaaattatt ttttagcaat
      181 aggccagcaa ataaaaagcc acccgaaggt ggcttaatgg ttggctaaaa ttgtttgcaa
      241 aaatattact ttttcaaaaa tgccgcaatg gcattaattt gtgcgtccgt cagggtggaa
      301 aaccttctca tagttgtcac attttgaata gccgttttga tttccgctgc ggtatggttg
      361 gtaacgtcat gaccagtatt tgaaccctgg ccattgcctt tgtggcagct gccgcagttg
      421 ctgacaaata atgctttgcc atcgattccg ccggaactgc tggagcttga ggagctagaa
      481 cttgaagaac tggacgagct tgaactggat gagcttgagc ctgggcaatt ggtgcctttg
      541 ggtgctgcat ccgatgtgcc ggtaacttcg gttttcttca gggcgttttg ctctaaccac
      601 tgtatttgtt gttgtacggt ggtgccagtt ggggtaacgg ataatgagcc cagagcatac
      661 acggtgaagg agtaggtatc tttctttcga gcgttgcacg acgttgacgg gcaggggcca
      721 aaaaacttcg tgccaccatt aaacgcactt ttttgcgtcg cgccgcccat ttcggtgggt
      781 ttagctgggg tggaaaggtt tttaggtacc atattgatgg attctggaat attccacatc
      841 aaccagtggt aaccgttggt ggggttggcg ctgttgataa tcgtcatgtc tttaaacgca
      901 acggcataac tcatggtgcc cgttgggcca tcggtccagt gaatttcggg ggaaatacca
      961 tcgccaaagt ttttaccttc gcaggtaaat tctgcaggaa tagcaccgcc ttcggtaaat
     1021 gaagtggtca tggcctgaaa gtttgttaag ccgcccgttg atgaactgct cgatgagcta
     1081 ctggatgaac tgctagagga cgagctgctg ctggaagagg agctgctaga ggatgacgac
     1141 gagcgccccg atgatgaact acttgagttg ctcgatgatg aactgctgct tgaagtgaca
     1201 tcaccgccgc caccgcaacc caccatagtg gctgacagca gggttatacc aacggctaaa
     1261 agagtcttat gcataaaaat tccgcctgaa catgagtgtg gagaccattg ataagccggg
     1321 ctgcgggcta accacttgtg tatcacctaa ggtattggcc tgcaccgacg cttgcggttg
     1381 ggttgtgtgt ttttatgtgt gccttcccgc ctgtgaatat ttcacggcag catgtaaaca
     1441 caattaaata ggtgctgggt tgccaatagg taagtttgct gagcgcacaa catgagaaat
     1501 gtgatctggg atttaattta tgttatttat cttttattga ggattgcaaa aattaaattg
     1561 actgactatt aaaataagaa ttcgtcagtg ttttgctggt tgtggaggcc tagtgttttt
     1621 tgccgttttt aaaaagtttt attgttggac aaaacatgca ggctaggtat tgaggcttgg
     1681 gaatttaatt ttttggaatg ggtaaatttt gctggcggcg agtgattatt tgggggaatt
     1741 aattctgggt tgttgcatgc aaggtttttg ggggttcacc tgcgcgtgta acaatacaag
     1801 ctgtgccgaa gcaatgaata cacattattt cagaccgtca acaagaggga cctggcggat
     1861 tcaaccccga aatgcatcat tcattaagcg gccttggccg ccagtttgtc attcattaaa
     1921 tccttcggtg ttctataccc taaaaccttg cggggccgct catttaactc atcgattacc
     1981 cgttttagtt gctcatcttc aaccaattta a
//
LOCUS       DAOQGS010000707         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_38483_length_2010_cov_4.685934, whole genome shotgun sequence.
ACCESSION   DAOQGS010000707 DAOQGS010000000
VERSION     DAOQGS010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38483_length_2010_cov_4.685934"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PKZ52_18010"
     CDS             complement(<1..1291)
                     /locus_tag="PKZ52_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61724.1"
                     /translation="MLALAPEYRMRVLRYSLLMGWLVLLAACWFDFGQSRITDPADSW
                     GISGFVGKPVEVRGQLLAPQAYNPAPRLFWGGVVPVVVLLLIFGGHDLWRRICPLSAF
                     SQIPRLLGWQRKVGKNGERQLALIQPNSFIARHTLLIQFVLFALGVTLRLTLTSANPR
                     ALVIFTLLTLCVAICVGFLWGGKTWCHYFCPMNVVQLALSGPRGLNAGKPVPGISQSM
                     CRKPGPSGDVSMCVACNSPCPDIDIERNYWQTLPKKTKRFVVYGYLGVVAGFIHGLYA
                     TAGSFSYGAAVWYDSDWQAIGFAPFGSFLAVPRMLGAAVLMLSWIGLAALIGFCLEAI
                     IYQAVNKQHGDSAREIAINRSMVISTASALLLLIFRVALPGLSWLNHELVEIVGWAAT
                     IAIIIWAHRSWSRTSSAHNKGLFVELCKTPAPDGKMTF"
     gene            1513..>2010
                     /gene="rsmA"
                     /locus_tag="PKZ52_18015"
     CDS             1513..>2010
                     /gene="rsmA"
                     /locus_tag="PKZ52_18015"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNG61725.1"
                     /translation="MAVLFEHAMTEHQARKRFGQNFLVDQRIINHIVRSIRPQAGDNL
                     VEIGPGKGAITALLLNACPNLNVVELDRDLIPILKATFSEYPNLVVHQADALKFDFSA
                     LATEQNPLRIVGNLPYNISTPLIFHLLSFRALVRDMHFMLQKEVVLRMAAQVGDNNYG
                     RLGIMT"
BASE COUNT          541 a          513 c          519 g          437 t
ORIGIN      
        1 ggaaggtcat tttgccatct ggcgcggggg ttttacacaa ttctacgaag agccctttat
       61 tatgggcact actggttcgg ctccagctgc ggtgtgccca gataattatg gcaatggtag
      121 ccgcccagcc aactatttcc accaactcat gatttaacca actcaacccc ggcagcgcca
      181 cgcgaaaaat taacagcaat aatgctgacg cggtggagat caccatgctg cggttgatgg
      241 caatttcccg cgcgctgtca ccgtgttgtt tattcacggc ttgataaatt attgcctcca
      301 aacaaaagcc gatgagcgcc gccaagccga tccagctgag cattaaaacc gccgcgccga
      361 gcattctggg cacggctaga aaagagccaa atggcgcaaa gccaatcgcc tgccaatcgc
      421 tgtcatacca caccgcggcg ccataactga aggaaccggc ggtggcgtac aaaccgtgaa
      481 taaaacctgc caccacacct aaatagccgt acaccacaaa gcgtttggtt tttttcggca
      541 gggtttgcca ataattgcgt tcaatatcta tgtcggggca gggtgaatta caggccacac
      601 acatggatac atcgccgctg ggaccgggtt tgcggcacat ggactggcta attcccggta
      661 cgggtttgcc agcatttaag ccgcgcgggc cggacagcgc caattgcact acattcatcg
      721 ggcaaaaata atggcaccag gttttgccac cccataaaaa accgacacaa atggcgacgc
      781 ataaggtgag tagcgtaaaa attaccaggg cgcgggggtt ggccgaggtg agggttagcc
      841 gcaaggtaac gcccaaggcg aataacacaa attgaattaa taaggtgtgg cgggcaataa
      901 aggagtttgg ttgtattagc gctaattggc gctcgccatt tttgcccact ttgcgctgcc
      961 atcccaacag acgggggatt tggctgaagg ccgatagggg gcaaatgcgc cgccaaagat
     1021 cgtggccgcc aaaaattaat aataaaacaa caacgggaac aacgccgccc cagaataagc
     1081 gcggcgctgg gttataggcc tgaggcgcca atagttggcc gcgcacttct acgggcttgc
     1141 caacaaatcc gctaataccc caactgtcgg ctggatcggt gatgcgcgat tggccaaaat
     1201 caaaccagca agctgccaat aatactaacc aacccattaa aaggctgtag cgcaggacgc
     1261 gcatgcgata ttcaggtgca agagctagca tggatacgag cccagggaaa aaccacaaac
     1321 cgcaggttaa acaatccaat atgtaggcgc aatttttaat ggggtattgg cgagcaatag
     1381 tagttttaat aaccgaagcc tgtaacccaa ccgggtcata ccggcgtttg agttctgaac
     1441 aggctcatcc tgatgggttg caattcatgg cgagcagtaa agcgctcgga cctgctgcca
     1501 ggttatgtca caatggcggt tttgtttgag cacgccatga cagaacacca agcacgcaaa
     1561 cgcttcgggc agaattttct ggtcgatcaa cgcattatta accatatagt gcgcagcatc
     1621 cgcccccagg cgggggataa tttagttgag attggcccag ggaaaggggc cattacggcg
     1681 ctgttattaa acgcctgccc gaatttaaat gtggtggaat tggaccgcga tttaattcct
     1741 atcctcaaag ctacatttag cgaatacccc aatttagtgg tgcatcaggc ggacgcttta
     1801 aaatttgatt ttagcgccct agccacagag caaaacccac tgcgcatagt gggcaacctg
     1861 ccctataaca tttccacgcc gttgattttt catttattga gctttcgcgc gttagtgcgc
     1921 gatatgcatt ttatgctgca aaaagaggtg gttttgcgca tggcggccca ggtgggcgac
     1981 aataattacg gccgcttagg catcatgacc
//
LOCUS       DAOQGS010000708         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_38739_length_2002_cov_4.059065, whole genome shotgun sequence.
ACCESSION   DAOQGS010000708 DAOQGS010000000
VERSION     DAOQGS010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38739_length_2002_cov_4.059065"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            742..>2002
                     /locus_tag="PKZ52_18020"
     CDS             742..>2002
                     /locus_tag="PKZ52_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNG61726.1"
                     /translation="MNLKYLVNGSLIAASLVLAGCGATDGSSSSSSSSSSSSSSSSSS
                     GSSSGLQCTAPKIERPTTSGKPVCLFPDEPYSYCTDSIYGNITVVYSSSSETFYSSLC
                     PPNRYSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSGATTGIQYRGVSLAAAEFGE
                     KQLPGIYDKDYTYPAQSSVDYFKSKKMNTVRLPFLWERLQPTLNQAFDNTEFARLDLF
                     VTETTAKGITVVIDPHNYARYRGNLIGSSQVPVSAFANFWSRLATAYKGNNKVIFALM
                     NEPNTMRTEDWLTAANAGAKAVRDAGANNLILEPGNAWTGAHSWTQNYYGTPNSQVMV
                     NFVDPKNNFAFEFHQYFDKDFSGTVAECATAEGDSKLAEATAWLKANNFRGFLGEFGG
                     GNTSTCQATIGRVLQYLESNKQWIGWTW"
BASE COUNT          483 a          500 c          495 g          524 t
ORIGIN      
        1 ccgccatatc gatgagctcc cggcagtcgg ccacaccttg atccaggttg tcgatggttt
       61 tcactaccag ttctaacgcg gctcgctctt tacccagggc ctgggcgcgc tcggggttgt
      121 tccacacatt gggctcggcc agctccagct ctacctcggt gaggcgctct ttgcgttcag
      181 cgtattcaaa gatacccccg aagggaatcg gcgcgggctt gcatgtcggt gagggcgttt
      241 aaaagggggt tgatttccat ggggattaaa gctcgtttac aaacaaaatg gcggcggatt
      301 gtaccctaat caaggcagtt tggggcggcg ctgccagggc ttaacgcacg gcaggtttgg
      361 gcagagtgga taagaaatgg taaaggcgcg cgcggtgtga agaatttacc cgctttgcct
      421 taagccaata aattatttgc gctaataacc aattccgtgt ttgcgcgctt gttggtcaat
      481 ttgtaaataa atagaatccc cggtgcttgg attcaagcga tggcttttgt ctgttttttc
      541 tcatcaacag gcctgtgaaa ttgcgagtga tttagtgctg aagtgctagg caagttctga
      601 tcaagtgtta attcgtaaat atttgaagcc tgtacgaata catgtgttag cgtcttaatt
      661 gggcagtcta accagatcga caagaaatca gaggaatcct agcgcgcaat ttacatagga
      721 cgtaacccaa cggatcctaa tatgaaccta aaatatttag tgaatggctc gctcattgca
      781 gcaagtttgg ttttggctgg ctgtggcgct actgacggga gcagctcatc tagctcgagt
      841 tcgtcatcga gtagttcttc gtctagctct tcaggctcct cgtcgggcct gcaatgtaca
      901 gctccgaaga tcgagcgacc gaccacttca ggcaagccgg tatgcttatt tcccgacgag
      961 ccttacagct attgtaccga ttctatatac gggaatataa ctgttgttta tagctcatct
     1021 tcggaaactt tttacagttc gctgtgcccg ccgaaccgtt attcaagcag ctcctcaagt
     1081 tcatcctcaa gttcatcctc aagttcatcc tcaagttcat cctcaagttc gtctagctcc
     1141 tcttcctcct ctagtacttc aggggctacc accggcatcc aataccgcgg tgtttcacta
     1201 gccgcagccg agtttggcga gaaacaattg cccggcattt acgataaaga ttacacctat
     1261 ccggcccaat cctcggtgga ttacttcaag agcaaaaaaa tgaacacggt gcgcttgccc
     1321 ttcttgtggg agcgtttaca gcccacgtta aaccaagcgt tcgataatac cgaatttgcc
     1381 cgcctggatt tattcgttac cgaaaccacg gctaaaggca taaccgttgt tatcgacccg
     1441 cacaactatg cgcgttaccg gggtaattta atcggttcca gccaggttcc tgtaagcgca
     1501 tttgccaatt tctggagccg tttggccact gcctacaaag gcaataacaa ggttattttt
     1561 gccttgatga atgagccaaa taccatgcgt acggaagact ggttaaccgc agccaacgca
     1621 ggtgccaaag ctgtgcgcga tgcgggcgct aacaacttga ttttagaacc tggcaatgcc
     1681 tggaccggcg ctcactcctg gacgcaaaat tattacggca cgcccaattc acaagtgatg
     1741 gtgaactttg ttgacccgaa aaataatttc gcctttgaat tccatcagta cttcgacaag
     1801 gatttctccg gtactgtagc tgagtgcgcc acagccgagg gtgatagcaa attggctgag
     1861 gcgacggcct ggttaaaagc caataatttc cgcggctttt taggcgaatt tggcggtggc
     1921 aacacctcaa cctgtcaggc aaccattggc cgggtattgc aatacttgga aagcaacaaa
     1981 cagtggatcg gctggacctg gt
//
LOCUS       DAOQGS010000709         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39095_length_1991_cov_5.315083, whole genome shotgun sequence.
ACCESSION   DAOQGS010000709 DAOQGS010000000
VERSION     DAOQGS010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39095_length_1991_cov_5.315083"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1426)
                     /locus_tag="PKZ52_18025"
     CDS             complement(134..1426)
                     /locus_tag="PKZ52_18025"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG61727.1"
                     /translation="MNKNIERSIKQMQAERRRFLDMLGKAGVSAGLLRASPIVGGLMS
                     ARFAEAATNDKKFILLYQPNGAPRNYLSSIALNPLKPYASTVAALTMNIRDPGNHGNI
                     HWATGAKSYNSSDANSSSIDLQVAKVLGNNTPKRSIQLGVQSGSQEGINRLNGAAVTR
                     IDSPSTALQQIFSGTSAGSSSSSTGSTSGPTDAERKLAIVDANKQGLDAIRNKLGYDE
                     RYRLDAHLDTITQLETRLKSQVSTGGSSSGSTGGSSGGSCTKPSVSSSKSALAEYRMQ
                     GDIAVAALACGITNVVSIQFNETQASWLPGDGTSDAVNFNADHHQVNHGGQAAALLPA
                     VCEYMNKGIANLIAKLQTAGIYNNTVILCVSEMGDGVNHTPDAGPIIVASGISGFRAG
                     NKAVGSDHFNVFPDIFKLLGVSGAAGIYGGYGSGGIVA"
     gene            complement(1438..>1991)
                     /locus_tag="PKZ52_18030"
     CDS             complement(1438..>1991)
                     /locus_tag="PKZ52_18030"
                     /inference="COORDINATES: protein motif:HMM:NF019256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HNG61728.1"
                     /translation="GDLTTREYYEIITNDPGCASCHQNRINPLFGIDDIDHLGKFRTT
                     MRGLGPNGQYNLPVNNYGELIGLASIDDTATLTFQGSKDLGKKMSTLPAISECLAVNS
                     FRWATGMPLNSNSYSTLEGGQDNEPAKLTRNQEESFACAEKTLQDTYDANNGSAKSLY
                     RKIGTLDLVRLRKPIDSSQVKQN"
BASE COUNT          463 a          480 c          526 g          522 t
ORIGIN      
        1 ttaaagaaaa aaccacacgc ggcaagtttt caattctcaa gagcgtttct gccgcagtgg
       61 cggtaaatga cgacaatata aaggcaaaaa aaaagccctc aaaagagggc tgggtgttag
      121 tttaatttaa tgcttaggca acgatgccac cactcccata tccaccgtaa atacctgcag
      181 cccctgatac tcccaataac ttgaatatat cagggaacac attgaagtgg tcgcttccta
      241 ctgctttgtt gccagctcgg aagccagaaa tacctgaagc aacgatgatg gggcccgcat
      301 ctggggtgtg gtttacacca tcgcccattt cactaacaca gagtatgacg gtgttattgt
      361 aaatgccagc tgtttgcaat ttggcaataa gattagcaat acccttattc atatactcac
      421 agaccgcagg caataatgca gctgcctggc caccgtggtt aacctggtgg tggtccgcat
      481 taaagttaac cgcgtctgag gtgccgtcgc cgggcaacca gctggcttgg gtttcgttaa
      541 attggatgga cactacgtta gtaatgccgc aggccaatgc tgcaacagca atatcacctt
      601 gcatacggta ttccgccaac gctgatttgc tcgaggatac tgaaggcttg gtgcatgaac
      661 cgcccgagga tccgccagtg ctaccactgg agctaccacc agtggaaacc tggcttttca
      721 aacgtgtttc caactgggta atggtgtcca agtgagcgtc caggcgataa cgctcgtcat
      781 agcccagctt gttgcggatg gcgtctaagc cttgtttgtt cgcgtccaca atagcgagct
      841 tacgctcagc atcagtcggc ccactggtgc tgccggttga gctgctgctg gagccagcgg
      901 acgtaccgct gaatatctgt tgcaacgcag ttgacgggct atcgattcgg gttaccgcgg
      961 caccattgag gcggttgatg ccttcttggc tgcccgattg aacgcctaat tgaatcgagc
     1021 gcttgggtgt attgttgccg aggactttag ccacttgtaa atcgatactg ctggagtttg
     1081 catcagaaga gttatacgac tttgcccccg ttgcccagtg gatattaccg tggttacctg
     1141 gatcgcgaat attcatagta agcgcagcta cagtgcttgc gtacggcttg agcgggttta
     1201 aagcaatact gctcaggtaa ttgcgcggcg caccattggg ttggtagagc aaaataaact
     1261 ttttgtcatt ggtagctgct tcggcaaagc gtgcactcat caacccgcca acgataggtg
     1321 aggctctgag caatccggca gaaacgcccg cttttccgag catgtcaaga aatctgcggc
     1381 gttcggcttg catttgctta atcgatcttt cgatattctt attcattctc atagccccta
     1441 attctgttta acttggctgg agtcgatggg tttacgcaaa cggaccaggt ctagagtacc
     1501 aattttgcgg tagaggcttt tggcgctgcc gttgttggcg tcataggtgt cctgaagcgt
     1561 tttctcggca caggcaaagc tctcttcttg attgcgcgtc aacttagctg gctcgttgtc
     1621 ttggccgcct tcaagggtcg agtatgagtt gctgttcagc ggcataccgg ttgcccagcg
     1681 gaagctgttt actgcaagac attcagagat agcgggcagt gtggacattt tcttgccgag
     1741 gtctttggag ccttggaagg ttaacgtcgc agtatcgtca atgctggcaa gaccaattaa
     1801 ctcaccgtag ttgtttactg gcagattgta ctgtccatta gggccaaggc cgcgcatggt
     1861 ggtgcggaat ttacccaaat ggtcaatatc atcgataccg aacagcgggt taatacggtt
     1921 ttggtggcaa gacgcacagc ctgggtcgtt ggtgatgatt tcatagtact cgcgagtggt
     1981 gagatctcca g
//
LOCUS       DAOQGS010000710         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39214_length_1987_cov_4.390787, whole genome shotgun sequence.
ACCESSION   DAOQGS010000710 DAOQGS010000000
VERSION     DAOQGS010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39214_length_1987_cov_4.390787"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(708..783)
                     /locus_tag="PKZ52_18035"
     tRNA            complement(708..783)
                     /locus_tag="PKZ52_18035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Ala,seq:ggc)
     gene            complement(909..>1987)
                     /locus_tag="PKZ52_18040"
     CDS             complement(909..>1987)
                     /locus_tag="PKZ52_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNG61729.1"
                     /translation="REAAGAPFVVRMMVPEAAGPCPVPDMLRGTIELDWSQVDAQVLL
                     KSDGLPTYHLANVVDDHLMQISHVIRGEEWINSAPKHILLYRYFGWQQPVFCHLPLLR
                     NPDKSKLSKRKNPTSILYYQRMGYLPEALLNYLGRMGWSMPDEREKFTLNEMQENFAI
                     ERVSLGGPIFDIDKLNWLNGLWIRENNTTEQLAARLVQWALNEQNLLAVIPQVHKRLN
                     TLSDFMPMVGFLLAGRLPLTASLFDEIKLDAETRIKVLQFASWRLEQLQAWDKDSIFA
                     ALKQLADGLGIKMKDFVAPIFIAISGSLTSFSVVDAMALLGPDMSRVRLREALELLGG
                     VSKKAAKQLEKDYAALVAPAAAEE"
BASE COUNT          570 a          525 c          426 g          466 t
ORIGIN      
        1 gtgctctatt acgggggcac agcactaaaa cacccacgcg tgagttccca gtcagggcag
       61 gaattacccc cagccacata aaaagatgct gaaaagttac atttttccta atttttcgcc
      121 cgcgcagata aaacaattaa taagacgcgc tcaatagtta caatttttag gccgcacaat
      181 tgatcagcgc gcaatcgcag aatttatcgt caagctgggt gctgatataa aatacctgga
      241 ataagccagg gttcgtaaat agttagaaaa aactatgcaa gcctattgcg ggcttttgcg
      301 ttggatgaat ttaaatacca ctgctctgaa gccaagcagg agtaccacat ctcattgatt
      361 aaaaataaat agccttgaac ctaaaaacca ctcttgggtg aaccacaata aaaaacacct
      421 aactatttag cacaccttat cgtgctcttt gcgagggcgg taaaatgagg gaccttattg
      481 acgagcctac acggatgtat tcacggcgca tctgaaataa gctgtattca ctgctgcggg
      541 cggagtacag atggctcgcg gcactaatat aaccggatgt tttctagtcg gcgcgagaat
      601 cacctccagc cacaataaaa agtgctgaat ggttaccaat aaacatccat acacaaaaca
      661 gaagccataa aaaaaccctc taggttgccc tagagggttt ttgaatttgg tggagcttag
      721 cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctaaagc
      781 cccgtcctag ccgtgtaccg gacagtggcg cgcattctag ggatgcatcc ctatcgagtc
      841 aaccccccaa ttagatttaa tgtaaaaaag cccatccatc cataacaagc cgcattttac
      901 ccgcgcccct actcctcagc agccgctggc gccaccagtg cggcataatc tttctccagc
      961 tgtttagccg cttttttact cacgccgccg agtaattcca gggcctcgcg caggcgcaca
     1021 cgggacatat ccggccccaa caaggccatg gcatccacca ccgaaaacga ggttaaggag
     1081 ccgctaatag cgataaatat aggcgccaca aaatccttca ttttaatgcc taagccatcg
     1141 gccaattgtt ttaaggccgc aaaaatagaa tccttatccc aggcttgtaa ttgctctaag
     1201 cgccaggagg caaattgcag cactttaatt cttgtctcgg catcaagttt tatttcatca
     1261 aataaactcg ccgttaaagg caaacggccc gccaacaaaa accccaccat aggcataaaa
     1321 tcgctcaggg tatttaagcg tttgtgcacc tgcggaatga ccgccaataa attttgctca
     1381 ttcagcgccc actgcaccaa acgcgccgct aattgttcgg tggtgttatt ctcacgaatc
     1441 cacaggccat tgagccaatt taatttgtcg atatcaaaaa tcggcccacc gagggaaacc
     1501 cgctctatgg caaaattctc ttgcatttca ttcagagtga atttttcccg ctcatcgggc
     1561 atagaccaac ccatgcggcc taaataattt aacagggctt cgggtaaata ccccatgcgt
     1621 tgataataca aaattgaggt ggggtttttg cgtttgctga gtttggattt atccgggttg
     1681 cgcaataaag gtaaatgaca gaataccggt tgttgccagc caaaataacg gtacaacaaa
     1741 atatgtttgg gggcggaatt aatccactct tcaccgcgaa tcacatggct gatctgcatt
     1801 aaatgatcat ccaccacatt ggctaaatga taagtgggca agccgtcaga ttttaataac
     1861 acctgggcgt ccacttggct ccaatccagc tcaatagtgc cgcgcagcat gtccggcacc
     1921 gggcaggggc ccgccgcctc cggcaccatc atacgcacca caaaaggcgc gccagcggct
     1981 tcccgac
//
LOCUS       DAOQGS010000711         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39230_length_1987_cov_3.093168, whole genome shotgun sequence.
ACCESSION   DAOQGS010000711 DAOQGS010000000
VERSION     DAOQGS010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39230_length_1987_cov_3.093168"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PKZ52_18045"
     CDS             complement(<1..1146)
                     /locus_tag="PKZ52_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HNG61730.1"
                     /translation="MITPKTLTALQAMSKIGTALAISLVLAACNSKNSSSGNSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGQAVNHAQNGNVECNTTG
                     WSARAATITRSVDDKHAGEGSLLVSGRGASWNGATYPLTTLVNGKTYQVSAWVKLAPG
                     EASEVIKITGKRADDSDNTTYLEYSTVGFGTVSADAWTLIEGTYTPDGVTPFESFIFE
                     TNSGAANEKVSFYVDDFVVSGEAATELAPFKCPKATTNELAVNGDVEDGINNWAGRGA
                     TIAANTEVKYADTASLYVSGRTASWQGATFSVSPIIAGATYNVSAWVKFGAEQATGTI
                     KLTGKLQDDADTGTYKEYSQIASATVTSGEWVKLEGQYVPGSTTPFES"
BASE COUNT          529 a          437 c          484 g          537 t
ORIGIN      
        1 cgattcaaat ggagtggtgc tgcctgggac gtattgcccc tcaagcttca cccattcacc
       61 tgaagttaca gtggcagatg cgatctgaga atactctttg taggtgccgg tatccgcatc
      121 atcctgtaat ttgccggtga gtttaatggt gcctgttgcc tgctctgcgc caaatttaac
      181 ccaggcggaa acgttgtagg tggcgccggc aattattggg ctcacgctaa aggtagctcc
      241 ctgccaactg gctgtgcgcc cagatacata aaggcttgcg gtatcagcgt atttaacctc
      301 tgtattagca gcaattgttg cgccacggcc agcccaatta ttaatgccat cctctacgtc
      361 gccgttgacg gcaagctcgt tagtagtagc ttttggacac ttaaaggggg caagttcagt
      421 agcggcttcg ccagacacaa caaaatcatc tacataaaaa ctaacttttt cattggctgc
      481 gcccgagttt gtctcaaaaa taaaactctc aaagggcgtg acaccatccg gagtataggt
      541 gccttcgatt aatgtccacg catcggcgga gactgtgccg aaacctacgg tgctgtactc
      601 taggtaagtg gtattgtcac tatcatccgc tcgtttgcca gtgatcttaa tcacctcaga
      661 ggcttcacct ggggcgagct tgacccacgc agagacctgg taggttttgc cattaaccag
      721 cgttgttaga ggataggttg caccattcca ggaagcgccg cgacctgaaa ccagcaagct
      781 gccctcaccg gcgtgtttgt catccacact gcgtgtaata gttgcagccc gggcgctcca
      841 gccagtggtg ttacattcca cattgccgtt ctgggcatgg tttacagcct gaccggagga
      901 actgcttgaa cttgaagagc tactcgagct actagagcta gaagagctag aagagctact
      961 agagctagaa gaactactag agctagaaga actactagag ctagaagaac tgctagagct
     1021 agaagaacta gaattgcccg aggaagaatt tttgctgtta caggcggcga gcactaggga
     1081 tatagctaat gccgttccaa ttttactcat tgcctgcaag gcagtaagtg ttttgggggt
     1141 tatcattttg atctcctaat tattatggat accaagtttt ggcaggtgag cgatgtgaca
     1201 atacaaaacc aactaaggat ataccagaaa atctaaattg ctcgctaaag tagaaaaatt
     1261 gtacaagcca ccaccggcag agggagacta atggggattt ttccgcaaat acacgcattc
     1321 gaccagaatt tttactaggc atgttatgga tcaaagtgtc tgatttgaat tgtttagcgc
     1381 tggtcgcaac agtgtgcatt tattgcgctt cgaatcaaca aagatcttta ggtgctcata
     1441 attttatcaa agcaggcagt tttaaattga tatttctagt cagagttaaa ttcaatctgc
     1501 ttattgtcaa agttacacat tggtattgaa tcggctttca tatcaaatac ggccattact
     1561 gttaatatgt gcggttattt tccttttaat tttaatccgt cttccctgct tggtggcagg
     1621 tttaatattc atgataacaa tatgattaat tgtgcgccgg atgccatgtt gatgtgtgcg
     1681 agcgcagtgc ttgtggcttt tggtggatgc tcacaggttg ggaacttata cgactagagg
     1741 aggccgcttg aaaccattag tcagcaacgt ccaccaaggt ggatgttgaa acagggcacc
     1801 acctgttatc tcggaactat tgaggctctc cagtgtgcta atcaagctga atgagtttct
     1861 atttaagaat tgccgcatta ttggggcagt acaaggcgca agctagcatt acaaggatca
     1921 tggtcagaaa acaaagcccg ctcggctggc agctgcgagg aaagaaaagc gcaataaatt
     1981 ggttgca
//
LOCUS       DAOQGS010000712         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39321_length_1984_cov_3.392950, whole genome shotgun sequence.
ACCESSION   DAOQGS010000712 DAOQGS010000000
VERSION     DAOQGS010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39321_length_1984_cov_3.392950"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PKZ52_18050"
     CDS             complement(<1..939)
                     /locus_tag="PKZ52_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNG61731.1"
                     /translation="MFSFYEAQSKVQKDRQLMRETDVRMAKYSRRGIVTNVLIYLLCL
                     ASGEFKEIEYNLSIILTIGLLLTTLLRGYFLYRFDSIYPRAPNRWRNIYFSVTLLGAA
                     WWGIITSSMTVALELQHEAPLIWLYTGVFFATTAHAFAPYGRFLTYYQLIGLVPLAIS
                     AFFVGELISLVSGVVMLFFCWILNHQSRTMTENYWDRLEANYILARKTETLEEEKRGT
                     RATVQLNQEYMGLLRNELKGLLETRRKPRDGSSEASELANNNRPRLLQLFNNVNDFYQ
                     VLTKELVIHNRVFNLRHLLQNLVAQYVNPAQVRSILL"
     gene            complement(1136..>1984)
                     /locus_tag="PKZ52_18055"
     CDS             complement(1136..>1984)
                     /locus_tag="PKZ52_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNG61732.1"
                     /translation="GAVEVLVAGHQCQAAAESLAACPAVGKVLLADSESLAHNLAEPL
                     AKLIVSQAGGYSHILAPATSFGKNLLPRVAALLDVQQISDVIAVVSADTFKRPVYAGS
                     LIATVQSTDAVKVLTIRTTAFDAAANTGGSANVEAIAATGSNNQSAFVSASLAVSERP
                     ELTAAKIIVSGGRALGSAENFELIYQLADTLGAAVGASRAAVDAGYAPNDYQVGQTGK
                     IVAPELYIAVGISGAIQHMAGMKDSKVIVAINKDPDAPIFQAADYGLVADLFEAVPAL
                     TQALKG"
BASE COUNT          446 a          488 c          599 g          451 t
ORIGIN      
        1 cagcagaata ctgcgcacct gggcggggtt aacgtattgg gcgacgaggt tttgcagcag
       61 gtggcgcagg ttaaatactc ggttgtgaat gactaattct ttggttaata cctggtaaaa
      121 gtcattcaca ttattaaata actgtagcag gcgcgggcgg ttattattgg cgagttcaga
      181 ggcttcactg ctgccgtcgc ggggcttgcg gcgggtttcg agtaggcctt ttaattcatt
      241 gcgcaaaagg cccatgtatt cttggtttaa ttgcacggtg gcgcgggtgc cgcgtttttc
      301 ttcttcgaga gtttcggttt tgcgagcgag aatataattg gcttctaacc ggtcccaata
      361 gttttccgtc atggtgcggg attggtgatt taaaatccaa cagaaaaaca gcatcaccac
      421 gccgctcacc aggcttatta attcgccgac aaaaaaagcg ctgatggcga gcggcaccaa
      481 accaattaat tggtaatagg ttaaaaaccg cccgtaaggc gcgaaggcgt gggcggtggt
      541 ggcaaaaaat acccctgtgt aaagccaaat taagggggct tcgtgctgta attcgagggc
      601 caccgtcatg ctggaggtaa taatgcccca ccaggccgca cccaataggg ttacggagaa
      661 ataaatattg cgccagcggt tgggtgcgcg cgggtagatg ctatcaaagc ggtagagaaa
      721 ataaccccgc agcagggtgg tgagtaacag gccgatggtg agaataatac tgaggttgta
      781 ttcaatttct ttaaattcgc cgctcgctaa gcacaacaga taaatcaata cgttggtcac
      841 aatcccccgg cgggaatatt tcgccatgcg cacatcggtt tcgcgcatca gctgccggtc
      901 tttttgaact ttggattgtg cttcgtagaa actgaacatg aagttgccta attaaaagag
      961 cgggcaggtt aaatagactc tagcctggct tatcgcaaaa ccacaaatcc ctgggctggc
     1021 cccagtatgg agttgttaca ccgcaggctg aggggcttaa tttttattgt gggggctgtg
     1081 tatcggtcta agtagcgcaa gtgcgctgcg cagccgaggc cgcgcagcgg gcgctttagc
     1141 ctttaagggc ctgggtgagg gcggggacgg cttcgaataa atccgccacc aggccgtaat
     1201 cggcggcttg gaaaatgggc gcgtccgggt ctttattgat agcgacgatc accttggagt
     1261 ccttcatgcc cgccatatgc tgaatggcgc cggagatgcc caccgcaatg tacaactcag
     1321 gggcaacgat tttgccggtc tgccccactt ggtaatcatt gggggcgtag cctgcgtcca
     1381 ccgctgcacg ggaggctcct acagcggcgc cgagggtgtc ggccaattgg taaatcagct
     1441 cgaaattctc ggcactgccg agggcgcggc cgccggagac aataattttg gcggcggtaa
     1501 gttcagggcg ctcgctgacg gccaggcttg cgctaacaaa ggcgctctgg ttgttgctgc
     1561 cagtagctgc gatggcttca acatttgcac tgccgccagt gtttgcagcg gcatcaaagg
     1621 cggtggtgcg aatggtgagc actttgacgg cgtcagtact ttgcaccgta gcgatcaggc
     1681 tgcccgcgta caccgggcgc ttgaaggtat cggcactcac cacggcgatc acatcggaga
     1741 tctgttgtac gtccagcagc gcagccacgc ggggcagcag gtttttaccg aagctggtgg
     1801 cgggggccag aatgtggctg tagccaccgg cctgggagac gattaatttt gccaagggtt
     1861 cagctaggtt gtgagcgagg gattcgctat cggccaacaa aaccttgccc acggcagggc
     1921 aggcggcgag gctctcagcg gcggcttggc actggtggcc cgctaccaat acttccacag
     1981 cacc
//
LOCUS       DAOQGS010000713         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39454_length_1980_cov_3.823896, whole genome shotgun sequence.
ACCESSION   DAOQGS010000713 DAOQGS010000000
VERSION     DAOQGS010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39454_length_1980_cov_3.823896"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKZ52_18060"
     CDS             <1..436
                     /locus_tag="PKZ52_18060"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNG61733.1"
                     /translation="PFGHGLSYSQFTYSDLTFERDQLAPTDTLKAKIKITNTSAVGGD
                     EVVQLYISDAVASLTRPVKELKAFKRIHLKAKQSRWLEFNVPVALMGFYSEALQYQVE
                     PGKITIQVGSSSQDIRQQKNLMITGDGPVSLPKQFFAQVREL"
     gene            596..1069
                     /locus_tag="PKZ52_18065"
     CDS             596..1069
                     /locus_tag="PKZ52_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61734.1"
                     /translation="MLRAQVGLLFLIMASCAWSAPEPAGATSAPPFGGKPGEALPPIP
                     ASLAGHWFNTENSATVGLIIQADKNCELYTERLTSPRSSRACKVELYRDETYLVYLKG
                     ANGQCGASADFEFRYLKSQQPSLERLDLDTGGAAHFLLERRLESPAVDTTGKKST"
     gene            1206..1628
                     /gene="ndk"
                     /locus_tag="PKZ52_18070"
     CDS             1206..1628
                     /gene="ndk"
                     /locus_tag="PKZ52_18070"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNG61735.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGQIYSRFESAGLRIVAAKMLTLSE
                     AQAKGFYAEHAERSFFGHLVSFMTSGPVVVQVLEGENAVAKNRDLMGATNPKEAAPGT
                     IRADFADSIDANAVHGSDSLTSAAREIAFFFASSDIAA"
     gene            1696..>1980
                     /locus_tag="PKZ52_18075"
     CDS             1696..>1980
                     /locus_tag="PKZ52_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNG61736.1"
                     /translation="MQDVSLPRVNLLGLTQPKLEAFFESLGEKRFRATQLLKWVHQLG
                     VLDFHAMTNMSKVLREKLSQVAELRLPEVVQQLDSTDGTRKFLIRVDGGSA"
BASE COUNT          448 a          562 c          508 g          462 t
ORIGIN      
        1 cccctttggc cacggcctca gctacagcca attcacctac agcgatttaa ccttcgagcg
       61 ggatcaatta gcccccaccg acaccctcaa ggcaaaaatt aaaattacca ataccagcgc
      121 tgtgggcggc gatgaggtag tgcagttgta catcagcgat gcagtggcaa gcctcacccg
      181 cccggtgaaa gagctaaaag cctttaagcg tattcacctg aaagccaagc aaagccgctg
      241 gttggaattt aatgtacccg tggcactcat ggggttttat tccgaggccc tacagtacca
      301 agtggagcct ggcaaaataa ccattcaagt gggcagttca tcccaggata ttcgccagca
      361 gaaaaacttg atgatcaccg gtgatgggcc ggttagccta cccaagcagt ttttcgccca
      421 ggtgcgcgag ttgtaggatt ggcaggagca cttacatagt gtgccctgta aaaagcccgc
      481 aatttttcca attgcgggct ttttaacact cggccccagg caatccctgc caaccattca
      541 cgcctgaaat taacccaaag gtttagaatg caggctttct ttgcggggat accctatgtt
      601 gcgcgcccag gtcggacttt tatttctcat catggcaagc tgtgcctggt ctgcccccga
      661 gcctgcaggc gccacaagcg cacccccgtt cggcggcaaa cccggcgaag ccctgccgcc
      721 gattccggcg agtctcgcgg gccattggtt taacactgaa aacagcgcca ccgtgggcct
      781 gattattcag gcggataaaa actgtgagtt atataccgag cggctcacca gcccgcgctc
      841 aagccgggca tgtaaagtgg agctgtaccg cgatgagacc tacttggtgt acctcaaagg
      901 ggccaatggg cagtgcggcg ccagtgcgga ttttgaattt cgctatctaa aatctcagca
      961 gccgtccctt gagcggcttg atttggacac cggcggcgcc gcccatttct tgctagagcg
     1021 gcggttggaa tccccggctg tcgacaccac aggcaaaaaa agtacctaat ttttaggctc
     1081 ttgccatcca gctgccccta ggctgtgcaa ttttgcaacg aacaccggta taatcgcggc
     1141 tttttcgctc cggtagccta taccggcgct ttatttttta tccatttcct aaagaggagc
     1201 aatccatggc tgttgagcgt accctgagca ttattaaacc cgatgccgtt gcgaaaaatg
     1261 tcataggtca aatttacagc cgatttgaat ctgccggcct gcgcattgtc gccgccaaaa
     1321 tgcttaccct gtcggaagcg caagcaaaag gcttttacgc tgaacacgcc gagcgctctt
     1381 tcttcggcca tttggtgagc tttatgacct ccggccccgt ggttgtacag gtgctcgaag
     1441 gtgaaaacgc ggtagctaaa aaccgcgacc tgatgggcgc caccaacccc aaagaagccg
     1501 cccccggcac catccgcgcc gactttgccg actccatcga cgccaacgcg gtgcacggct
     1561 ccgactcctt aacctccgcc gcgcgggaga tagcgttttt ctttgcatct agtgatattg
     1621 cggcgtaatt gccgcgttta agctgagttt tcccgtttca tttgcagtcc gtttcattcg
     1681 gttataggta ttgccatgca ggacgtaagt cttccccgcg tcaatttact tgggcttacc
     1741 cagccgaagc tggaggcgtt ttttgaatcc ttaggggaaa aacgctttcg cgccacccag
     1801 cttttaaagt gggtgcatca gttgggtgtg ctggactttc acgccatgac caatatgagc
     1861 aaagtgttgc gggaaaaact cagccaggtt gctgagctgc gtttacctga agtggtgcaa
     1921 cagttggatt ccaccgacgg cacccgcaag ttcctcattc gagtggacgg cggcagcgcc
//
LOCUS       DAOQGS010000714         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39511_length_1978_cov_4.461778, whole genome shotgun sequence.
ACCESSION   DAOQGS010000714 DAOQGS010000000
VERSION     DAOQGS010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39511_length_1978_cov_4.461778"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PKZ52_18080"
     CDS             complement(<1..1356)
                     /locus_tag="PKZ52_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNG61737.1"
                     /translation="MLILPGAPALSAFRSHKLLQTLQALDQGVSGIYAEYVHLADTNG
                     PLSPSETEILVQLLRYGPSVKAQMGVGQLLLVVPRPGTISPWSSKATDIAHNAGLARV
                     ARLERGIAYHVQGDFDLNKVLPALHDRMTESVFASLDEAAALFAKQEPRELSIVDVIG
                     GGRIALEVANTQLGLALADDEIDYLVSSFTQLGRNPTDVELMMFAQANSEHCRHKIFN
                     ASWTLDGVEQPHSLFKMIKHTYERGGENVLSAYADNASVIAGPSAGRFYPDPKTREYG
                     YHQEPIHILMKVETHNHPTAIAPFSGAGTGAGGEIRDEGAVGKGSKPKAGMTGFTVSN
                     LQIPGFIQPWEQNYGKPERIVSPLTIMIEGPLGGAAFNNEFGRPNLAGYFRTFEESFD
                     GERRGYHKPIMIAGGYGNIKEEHIAQTPFSPGTKLIVLGGPAMLIGLGGGAVQGGGAV
                     ID"
     gene            complement(1444..1842)
                     /locus_tag="PKZ52_18085"
     CDS             complement(1444..1842)
                     /locus_tag="PKZ52_18085"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNG61738.1"
                     /translation="MPIRFAQQFFAGSLGKFLLVGGLATAAQFATLFVLVELGLCREL
                     TASGLGYGVGAVVNYLLNYYVTFNSKQNHLATLPKFIAVVAFGLSVNTLVFYLANLVL
                     PWYGLSQCVATGASLVANYLLHRFWIYRKG"
BASE COUNT          455 a          548 c          580 g          395 t
ORIGIN      
        1 gtcgatgacg gcaccgccac cctgcaccgc gccaccgccg agaccgatca gcatagccgg
       61 gccacccaac acaatcagct tggtgccagg gctaaaagga gtctgcgcta tgtgttcttc
      121 tttgatattg ccgtagcccc cggcaatcat gatgggcttg tggtaacccc gccgctcgcc
      181 atcgaagctc tcttcaaagg tgcgaaagta accggcgaga ttagggcggc caaattcgtt
      241 attaaacgct gcgcccccca aggggccttc gatcatgatg gtaagcggag agacaatgcg
      301 ctcaggcttg ccgtaatttt gctcccaagg ctgaataaag cctggaatct gcaggttgga
      361 gacggtaaac ccggtcatcc ccgccttagg tttagagccc ttgcccaccg cgccttcatc
      421 gcgaatttca ccaccggcgc cggtgcccgc ccccgagaaa ggcgcaatgg cggtggggtg
      481 gttgtgggtt tccactttca tcaggatgtg aatcggctcc tggtggtagc cgtactcgcg
      541 ggtttttggg tccgggtaaa agcgcccggc gctggggccg gcaatgacag aggcgttatc
      601 ggcataggcc gaaagtacat tctcgccgcc acgctcatag gtgtgtttga tcatcttaaa
      661 aagcgagtgg ggctgctcaa ctccgtctaa ggtccagctg gcattgaata ttttgtggcg
      721 gcagtgctcg gagttggcct gggcgaacat catcaactca acgtctgtgg ggttgcggcc
      781 cagctgggtg aaactgctca ccagatagtc gatttcgtca tccgccaggg ccaggcccag
      841 ctgggtattg gccacttcca acgcaatgcg gccaccgccg atgacatcca caatggataa
      901 ctcccggggc tcttgtttgg cgaacagggc cgccgcctca tcgaggctgg caaacacgct
      961 ttcagtcatg cgatcgtgca gggcggggag cactttgttc aggtcaaaat ccccctgcac
     1021 atggtaggcg atgccccgct ccaggcgagc cactcgggct aaaccggcat tgtgggcaat
     1081 atccgtggcc ttagaggacc agggtgaaat ggtgccgggg cgtggcacca ccagcagtaa
     1141 ctggccaacc cccatttggg cttttaccga ggggccgtaa cgcagcaact gcacgaggat
     1201 ttccgtttcg ctggggctta aggggccgtt ggtgtccgcc aggtgtacat attcggcata
     1261 aataccgcta acaccctggt cgagggcttg cagcgtttgc agaagtttgt ggctgcgaaa
     1321 cgcagacaag gcgggggcac cgggcaaaat cagcataggg ttgaaatctc gctcaacaaa
     1381 aaagaaaaag gcgccattgt acttgatggc cgctcaaaga gtcagtggtg aaataccgag
     1441 gatctagccc ttgcggtaga tccagaagcg atgcaatagg tagttggcca ccagggaggc
     1501 gccggtagcg acgcattggc ttaagccata ccatggcagc actagattgg ctaaataaaa
     1561 tactagggtg ttcacgctta aaccgaaggc caccacagcg ataaacttcg gcagggtcgc
     1621 caggtgattc tgtttactgt taaacgtgac gtaataatta agcaggtaat tcaccaccgc
     1681 ccccacccca taccccaatc ccgaggcggt aagttcgcgg cacaggccaa gctccaccag
     1741 cacaaacaaa gtggcaaatt gcgcggcggt agcaaggccg ccgactagta aaaacttgcc
     1801 tagggaaccg gcaaaaaatt gctgggcaaa acggatcggc atgggcgggg cctgacaaat
     1861 cgggcgcgca gtttaacagc gctgctcgat ctgtgcagtc ctgcactgcg gcgttaaacc
     1921 taaaaaccgc agttgaactc gtagcgaggg tgcctaaggc gccgaatatg ggcgcttt
//
LOCUS       DAOQGS010000715         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39651_length_1974_cov_3.817092, whole genome shotgun sequence.
ACCESSION   DAOQGS010000715 DAOQGS010000000
VERSION     DAOQGS010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39651_length_1974_cov_3.817092"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKZ52_18090"
     CDS             <1..798
                     /locus_tag="PKZ52_18090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNG61739.1"
                     /translation="PLDAITVTCLRCGIAFFALLAFALWQGQPWGFKTHRERCLVVGL
                     GVLMGIHWSCFFKGMQVSTVAIGILAHYTTPVVTVTLEPLLDRQLPKRADVIAALLVL
                     VGLAIMVPQWHWGSQTLWGIFFGVCSALTLASRNVFQRRWLNNTSSVQSMVIQVAVAA
                     LVCAPFSQFDQLGPLTPTDWWLILGLGLVSTAFAHTLIVVSLQHLAAKSVSLISCFQP
                     PLAILLGWLILHEAPNLNTLLGGSLILGCALYEAIKQSSPAADIKAD"
     gene            complement(826..1719)
                     /locus_tag="PKZ52_18095"
     CDS             complement(826..1719)
                     /locus_tag="PKZ52_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNG61740.1"
                     /translation="MRHAISLEALRILDAIERKGSFGAAAEAIHKVPSALSYTVAKLE
                     ADLGVSIFDRSGQRAVLTAAGRLLLEEGRHLLAAAERLEALIKQLESGWEPVLRIARD
                     TILPMAPLLGVLGQFNQLDKQVEISLSDEVLGGSWDALVAERCDLILGAGGELPRGHF
                     EYRSIGEVEFVFAVAAGHPLTGRQAPVDQAAIRDLPTIVVADSSRSLPGRSSGLLDGR
                     HTIRVASMQAKLDAQVMGLGVGFLPKHLAEPALQNGSLIALPCTIPRPNMPAYMAWRK
                     DNKGLALQWFVQACAAVRWFL"
BASE COUNT          417 a          615 c          510 g          432 t
ORIGIN      
        1 ccattggatg ccatcaccgt aacctgcttg cggtgtggta tcgctttttt tgcgctgcta
       61 gcctttgccc tctggcaagg gcagccctgg ggatttaaaa ctcaccgcga gcgctgtttg
      121 gtagttgggc tgggggtatt gatgggcatc cattggagct gtttcttcaa aggtatgcag
      181 gtgtccaccg tggccatcgg catactggcc cactacacca cgccggtggt aaccgtcacc
      241 ctggaacccc tcttggatcg acaactgccc aagcgcgcgg atgttatcgc cgcgctcttg
      301 gtattggtcg ggcttgcgat tatggtgccc cagtggcact ggggcagcca aaccctgtgg
      361 ggcatctttt ttggtgtctg ctccgcgctt acactcgcct cccgcaatgt gtttcagcgc
      421 cgttggctga ataacacgtc cagtgtccaa agcatggtga ttcaggtggc ggtggcggct
      481 ctggtgtgcg cgcccttcag ccaatttgat caactaggcc cgcttacccc caccgattgg
      541 tggctgattc tgggtttggg gctggtcagc acagcgtttg cccacacgct gattgtggtg
      601 agcctacaac acctagccgc caaatccgtc agcctcatca gttgctttca gccgcccctg
      661 gccatcctgt taggctggtt aattctgcat gaagccccca acctcaatac cttgctcggc
      721 ggctcgctta ttttgggctg tgcactctat gaagccatca aacagagcag cccggccgcc
      781 gacatcaagg ccgattaagg atcctaaaaa ccgcagtttc taggattata aaaaccagcg
      841 cacagcggcg caggcctgta caaaccactg cagcgccaag cccttattgt ctttgcgcca
      901 ggccatataa gcgggcatat tcgggcgcgg aatggtacag ggcagcgcga taagactgcc
      961 gttttgcaaa gcgggttcgg ccagatgctt gggcaaaaac cccaccccca agcccatcac
     1021 ttgagcatct aatttcgcct gcatactggc cacccgaatg gtgtggcgcc cgtctaataa
     1081 acctgaggag cgccccggta ggctgcggga ggaatcggct accactatgg tgggcaggtc
     1141 gcgaatggcc gcctgatcca cgggcgcctg ccgccctgtt aaggggtggc ctgccgccac
     1201 cgcaaagaca aactcaacct cgccgatgct gcggtattca aaatgcccgc ggggcaactc
     1261 accgcctgcg ccgagaataa gatcgcagcg ctcggccaca agggcatccc aactgccgcc
     1321 gagcacctcg tcgctgaggc taatttccac ctgtttatcc agctgattaa actgcccgag
     1381 cacccctaaa agcggtgcca taggcaaaat cgtatcccgt gcaatccgca gcaccggttc
     1441 ccagccgctc tccagctgtt taatcagcgc ctccaaccgc tccgccgccg ctaacagatg
     1501 gcggccctcc tccaacaaga ggcggccagc cgccgttaat accgcccgtt ggccgctgcg
     1561 atcaaaaata ctcaccccca aatccgcttc aagcttggcc accgtgtagg acagtgccga
     1621 gggcactttg tgaatcgctt cggcggcggc gccgaaactg cccttgcgct cgatggcatc
     1681 gagaatccgc agggcttcaa ggctgatggc atggcgcatt ggggtttacc ttatcaattc
     1741 aatttttttg aacataccag tcaaaatctt ccgctttttt aaccgggctg ttcaacctaa
     1801 agtaacttca acaggcaata aaaccaacgg gttgccagaa ctcttaatca aattaattga
     1861 ggaatacacc atgaatcgca tgcaaaccct actcaacacc caagccaccc tcgcccccct
     1921 ggcactgcgt ttacccctag gggttatttt tgccgcccac ggcgcacaga aatt
//
LOCUS       DAOQGS010000716         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_39946_length_1966_cov_3.964417, whole genome shotgun sequence.
ACCESSION   DAOQGS010000716 DAOQGS010000000
VERSION     DAOQGS010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39946_length_1966_cov_3.964417"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..376
                     /locus_tag="PKZ52_18100"
                     /pseudo
     CDS             29..376
                     /locus_tag="PKZ52_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
     gene            373..>1966
                     /gene="rsxC"
                     /locus_tag="PKZ52_18105"
     CDS             373..>1966
                     /gene="rsxC"
                     /locus_tag="PKZ52_18105"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNG61741.1"
                     /translation="MSKLWPLSGGIHPPQNKQPTATKPIGRVPLPERLYLSLLQDRGA
                     QLGEALRPLVVPGERVLKGQILATAAGAAAVPIHAPASGTIGDLVQRPLNHPSGHLGP
                     HLELIRDGREQWCTLAPMEDPFSCPKPQALRLIQQAGICGMGGAGFPTALKLATDQPI
                     HTLILNGTECEPYITADDRLMQEAPSDILLGALLCAHILGAGSIKLAIEDNKPAAIRA
                     MASAIKSQPEAPPVHIELAVLPTIYPSGGTKQLVQILTGQQVPSGQQAASLGLLVLNV
                     ATARAVWRALRHGEPLIERVTTFAGAALKVPQNLWVPLGTPAEHLLTQLGWQSAPTLI
                     LGGPLMGFNLNPGASLGKTSNCVIAPSGEEMPPTVSEAPCIRCGQCDSVCPAQLLPQQ
                     LFWYAKAEDSARLLTHNLFDCIECGACSYVCPSTIPLVQYYRHAKADIRAQQANKLKA
                     DRARSRFEAHQARLAEQEAEKERKRIARQSATAAINAELAQTQVPVAAAPAAPEELAA
                     KLQRHQQSAQERLARLQQQAAEE"
BASE COUNT          422 a          654 c          547 g          343 t
ORIGIN      
        1 ggggctgcgc ttctaccccg agtaaatggg ccaagccacc atcgacgccc tggcccattt
       61 actcggggta gaagcgcagc ccctagacgc cgaacacggc gttgaaagcc aggttaaaac
      121 cgtggcgttt attcgcgagg cggagtgtat cggctgcacc aagtgcattc aggcctgccc
      181 ggtggatgcc atactcggcg cggccaaact catgcacacc gtcatcgcta gcgaatgcac
      241 cggctgcgac ctctgtgtgg agccctgccc ggtggattgt attgaaatgc gcccagtgcc
      301 aagtgagctt ccggcctggc cctggccaaa acccttggcc gaggccgccc ttatcgccag
      361 cgaccgccaa ccatgagcaa actctggccc ctaagcggcg gcattcaccc gccgcagaat
      421 aaacagccca ccgctactaa accgattggc cgcgtgcctc tgcctgagcg cctgtattta
      481 agcctgttgc aggatcgcgg tgcccagctt ggcgaagccc tgcggcccct ggttgttccc
      541 ggtgagcgtg tactaaaagg ccaaatactt gccaccgcag caggtgccgc tgcggttcct
      601 atccacgcgc ccgcctcggg caccataggc gatctggtgc agcggccact taatcacccg
      661 tccggccatt taggcccaca cctagaatta atcagggatg ggcgtgagca gtggtgcacc
      721 cttgcgccca tggaagatcc gtttagctgc ccaaagccac aggcgttaag gctaattcag
      781 caggctggca tctgcggcat gggtggcgca ggcttcccta ctgcgcttaa attagccacc
      841 gaccagccca ttcacacatt aattctcaac ggcaccgagt gcgaacccta tatcacggcg
      901 gatgaccgcc tgatgcagga agcgcccagc gatatcctgc tgggcgccct gctctgcgcc
      961 catatattgg gagcgggcag tattaagctt gccatcgaag acaataaacc cgccgccatc
     1021 cgcgctatgg cctccgctat aaaatcccag ccggaagctc cacccgtcca catcgaactt
     1081 gctgtacttc ccaccattta cccctccggc ggcactaagc agttagtcca gatattaacg
     1141 ggccaacagg tgcccagcgg ccagcaggcc gcaagccttg ggttattggt gttaaacgtg
     1201 gccaccgcgc gggccgtgtg gcgcgccctg cgccatgggg agcctttgat tgagcgggta
     1261 accacctttg caggggcggc tcttaaggtg ccgcagaacc tatgggtgcc cctgggtaca
     1321 ccagccgaac acctgcttac ccaactgggc tggcaatccg ctccaacgct aatactcggc
     1381 gggccgctga tggggtttaa cctcaaccca ggggcgagcc ttggcaaaac cagcaactgc
     1441 gtcatagccc ccagcggcga ggaaatgccc cccacggtat cggaggcccc ctgtattcgc
     1501 tgcggccaat gcgacagcgt ctgcccggcg caactattgc cccaacagtt attttggtac
     1561 gccaaggccg aggacagcgc aaggctactc acccacaacc tgttcgactg cattgaatgc
     1621 ggcgcctgca gttatgtatg ccccagcact attcccctgg tgcagtatta ccgccacgcc
     1681 aaggccgata tccgcgctca gcaggccaat aaactcaagg cggaccgcgc ccgcagccgg
     1741 tttgaagccc accaagcgcg ccttgcagaa caggaagcgg aaaaagaacg caagcgtatt
     1801 gcccgccaga gcgccaccgc agccattaat gcagagcttg cccaaacgca agtgcctgtg
     1861 gccgcagccc cagcggcgcc ggaggagtta gcggcgaaat tacagcgcca ccagcaaagc
     1921 gcccaggagc gcctggcgcg cttgcaacaa caagcggccg aagaac
//
LOCUS       DAOQGS010000717         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_40066_length_1962_cov_4.531201, whole genome shotgun sequence.
ACCESSION   DAOQGS010000717 DAOQGS010000000
VERSION     DAOQGS010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40066_length_1962_cov_4.531201"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKZ52_18110"
     CDS             <1..246
                     /locus_tag="PKZ52_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61742.1"
                     /translation="PELTSLKGNGNQLLEWRYEKLPKLEKVMLFNNKMPSIDIYHLPA
                     LKIMDVRQNPMPDPLYDAMNKMTGITFMHDGNAPDWK"
     gene            760..1776
                     /locus_tag="PKZ52_18115"
     CDS             760..1776
                     /locus_tag="PKZ52_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61743.1"
                     /translation="MASGWQNQHSDGVRFNGEPRIPDVPGGDINQLFLRYGSATQQLT
                     LGRQTLEWDDLRFLSANNFWQNPQSFDAFHIDRSLAETSHIQYAYISKSLRIFGRDAD
                     TRLQLSDINYHSLKGQRPRDGLGEHQMHSHALKWQWREWDYTDLTVYGYFINNETAAN
                     FSNDTIGARLGYQQKFSNWNLRFSFAGARQKRPHFSQDYDLPYWHLQNSIGQGAYEWS
                     LDYEYLGAHNGKALITPLGFSHEYQGWADRFTSTPTKGVEDISLRNTSRWGRLRMDIR
                     YHQFFSVQDNDQYGHEMDAELGYQFAVNQKIALRMARFWAEPEYKNSLKSVRNFYLTG
                     NYQY"
BASE COUNT          559 a          478 c          413 g          512 t
ORIGIN      
        1 cctgaactta ccagcctaaa gggcaacggc aatcaattac tggaatggcg ctatgagaag
       61 ttacccaagc ttgaaaaagt catgctattc aataacaaaa tgcccagcat cgatatttat
      121 catttgcccg cgttaaaaat tatggatgta cgacaaaatc caatgccaga ccctttatac
      181 gacgccatga ataaaatgac cggcattacg tttatgcatg atgggaatgc gccagactgg
      241 aaataaatcc ttccttcacc aatagtgcgt ttagttgatg caggttaagc agattgtatt
      301 acctatagcg cctaaatatg cgtaaaatac attttttacc gacatatccg ctctatgaac
      361 aagcgctatt gtgaaactac tacggagcct tcgcttaagt ctggaggtgt tgatatttca
      421 aaactatcac tggcagtatg cctgcgacgg agctacagga aggcacggct aggttttcac
      481 actgttcaca ctttcagact taatcaaagc ccccctcgat ttagcagagc gataaaacgt
      541 gatagcacag cattttctct attcgccgca tccttatttt tctatgcccc ttcactatca
      601 gcccaaccat tagaattagg cgctcgtgca cgtctagccc atttaactga atataaccaa
      661 gatactagta ttcaggggca ggctgccagc ctcgcactgc gtgccaactg gcaagccaat
      721 tggagcaatg ccatcacaag cttcattcag gctgattggg tggccagtgg ctggcaaaat
      781 caacacagcg acggcgtgcg ttttaatggt gaaccgcgta ttcctgatgt gccaggtggc
      841 gatattaacc aattatttct gcgctatggt tcagcaacac aacagctcac actggggcga
      901 cagaccttgg agtgggatga tttgcgtttt ttaagtgcca ataatttttg gcaaaatccg
      961 cagagttttg atgcctttca tattgatcgc tccctagcag aaacctccca tattcaatac
     1021 gcctacatta gtaaatccct gcgcatcttc gggcgagacg ctgatacccg tttacagtta
     1081 agcgatatta attatcacag cctgaaagga caacgcccta gagatggatt gggtgaacac
     1141 cagatgcact cacacgcgct gaaatggcaa tggagagaat gggattacac cgatttaacg
     1201 gtttatggct actttatcaa caatgaaact gcagccaatt tttccaacga taccatcggt
     1261 gcgcgcctag gttatcaaca aaagttctct aattggaatt tacgtttttc attcgcaggc
     1321 gcaaggcaaa aacgcccgca cttcagccaa gattatgatt taccttattg gcatctgcaa
     1381 aatagtatcg ggcaaggcgc ttatgagtgg tcactggact acgaatactt gggcgctcac
     1441 aatggtaagg cattaattac ccctctaggc tttagccacg aataccaggg ttgggccgat
     1501 cgctttacct caacccccac taaaggcgtt gaagatatta gcctgcgcaa caccagccgt
     1561 tggggccggt tgcgaatgga tattcgctac caccaatttt ttagcgtgca ggacaatgac
     1621 caatatggcc acgaaatgga cgcggagttg ggttatcaat ttgccgtcaa ccaaaaaatt
     1681 gctttgcgta tggcacgatt ttgggctgag cctgaatata aaaatagcct taaatccgtg
     1741 cgcaattttt atttaaccgg caactatcaa tactgattca tataacccag agcaggaaaa
     1801 ccaaatgaat tggcacgcct tgcctcagtt ctcagccatt taataaatgc aagcgccccg
     1861 aaataacatc caaataggta acttctgatc cccccatcca tgggctcgtc gatttcattc
     1921 ccgatgaaac tctacctccc caatccatgg ggtcgtcgat tt
//
LOCUS       DAOQGS010000718         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_40069_length_1962_cov_4.353435, whole genome shotgun sequence.
ACCESSION   DAOQGS010000718 DAOQGS010000000
VERSION     DAOQGS010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40069_length_1962_cov_4.353435"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            643..>1962
                     /gene="secD"
                     /locus_tag="PKZ52_18120"
     CDS             643..>1962
                     /gene="secD"
                     /locus_tag="PKZ52_18120"
                     /inference="COORDINATES: protein motif:HMM:NF025098.2"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNG61744.1"
                     /translation="MKPYPLWKYLALIAATVLGAIYSLPNLYLPDPAVQVSGYSSRIK
                     VDQVISDKAVESLKAANITVLHAEMGKEGVMIRLTKAEDQLVAQDVLDKALNDQSGKK
                     GADYVIALNKASTTPAWLEKLGAKPLNLGLDLAGGIHFVLEVDTPKSVNDRMLDIEEV
                     VKKAIRDARIFDVDLKHSGTEFILGVKDEAARDSVTKILQESARELQHTTTGDAATGF
                     IIKATLTEVAIREIEEHAISQNLTTLRNRVNELGVAEPIVQRQGRNRIVVELPGLQDP
                     ARAKAIIGKTANLEFRLEANATSTSKEKFDFRNEADQARFGGAVLERRPIVTGEAVVG
                     ATRGYDQQTGQVQVNISLSGDGGAEMHRATRNNVGRKLGVVFIEYRTSKSMVRNDKGE
                     LVEQTVQIPERHCISLATIQSALGREFRTTGLTEDEAGELALLLRAGS"
BASE COUNT          458 a          502 c          546 g          456 t
ORIGIN      
        1 gccctctgta tggggtaact gttgtaaatt tcagtttagc cgggccgtgc aattgtgcaa
       61 attggttaag ttttctgcgc gcgactgtgc aaatggaaat aagcctggaa ttgttggcgg
      121 gctggtgctg tgcacattgg gaatattctg agttggtctg aaatctgagt cagtctgtga
      181 gaaatctgag tcagtctgta aatggttgcc cactatggtc acctcagact taatcagagc
      241 ttcacctagg ttgagtcctc tcctccgggg agtgaaacct aaaaaccctg tttgcccctg
      301 ctgcggttga attgcatgtt ttggcttaaa tggcataagg cgagcagcgt cgcccctgca
      361 aacactacga ggggttcatt tcttggtgct ctttaatcca tgccacctat ttaagccggg
      421 gttatagcgt ctcacgcaag ggatagcgcc gccctgttgc attcgcggcg gcttggcggt
      481 ggcatggatc ggcatatgaa tgccattttc ttatgttacc ttgaatagtc gctcactcat
      541 cctcataaaa aggcggctat aatcgccgcc cttgatcagg cccgagcccc cagggcagca
      601 ctagaaaagc tataaacgat tcttcatctg ttggacctat gcatgaaacc ctatcctctc
      661 tggaaatacc tcgcccttat cgccgcaacg gtgctaggtg ccatttactc gttgcccaat
      721 ctttatttgc ctgaccctgc cgtacaggtg tccggttact ccagccgcat aaaggttgac
      781 caggttattt ccgataaggc ggttgaaagc ctaaaggcgg ccaatatcac ggtattgcac
      841 gccgagatgg gcaaagaagg ggtgatgatt cgcctgacca aggccgagga tcagctggtc
      901 gcccaggatg ttttagataa ggccttgaat gaccagagcg gtaagaaggg tgctgattat
      961 gttatcgccc tcaacaaggc gtccacgact ccggcctggc tggaaaaact cggtgccaag
     1021 cccttaaacc tggggcttga cctggccggt ggcatccact ttgtgttgga ggtagatacc
     1081 cctaagtcgg tcaatgaccg catgctggat atcgaagagg tggttaaaaa agccattcgc
     1141 gacgcgcgta tatttgatgt ggaccttaaa cacagtggaa ctgagtttat cctgggtgta
     1201 aaagacgaag cggcccggga ttctgttact aaaattctgc aggagtctgc ccgcgagctg
     1261 caacacacga caacgggtga tgccgctacc ggcttcatca ttaaagctac gcttacggaa
     1321 gtagccatcc gcgaaattga agaacacgcc atctcacaaa acctcaccac cctgcgcaat
     1381 cgggtgaatg agttgggggt tgccgagccc atagtgcagc gccagggccg caaccgcatt
     1441 gtggtggagt tgcccggctt acaagacccc gcgcgggcca aagccattat cggtaaaacc
     1501 gccaaccttg aattccgtct ggaggccaat gccacctcca ccagcaaaga aaaattcgat
     1561 ttccgcaatg aggccgatca agcccgcttt ggcggcgcgg tgttagagcg tcgccccata
     1621 gtcaccggtg aagctgtggt gggtgccacc cgcggttacg atcagcaaac cggccaggtg
     1681 caggtgaata tctcgttaag tggtgatggc ggtgcggaaa tgcaccgggc cacgcgcaat
     1741 aacgtcggcc gcaagttggg ggtggtgttt attgagtacc gcaccagcaa aagcatggtt
     1801 aggaatgaca agggcgagtt ggttgaacag accgtgcaaa ttcctgagcg ccactgtatt
     1861 agcttggcca ccatccaaag cgccctgggg cgggaatttc gcaccacggg tttaaccgaa
     1921 gatgaggccg gtgaactggc cctgttgctg cgggcaggtt cc
//
LOCUS       DAOQGS010000719         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_40070_length_1962_cov_4.135291, whole genome shotgun sequence.
ACCESSION   DAOQGS010000719 DAOQGS010000000
VERSION     DAOQGS010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40070_length_1962_cov_4.135291"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1962
                     /locus_tag="PKZ52_18125"
     CDS             <1..>1962
                     /locus_tag="PKZ52_18125"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNG61745.1"
                     /translation="LIELLDNSELAALAAEELKHTLLMFDRFHDVEEKARAGNTHAKA
                     VLQSWADAEWFTSKPAVAESIKLAVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMYK
                     MARDGLTPDEQGAKGPLKQIEAIKAKGIPVAFVGDVVGTGSSRKSATNSVLWFFGDDM
                     PGVPNKRTGGVCIGSKVAPIFYNTMEDAGALVFEAPVDKIAMGDIIEVRPYDGKILSE
                     SGALLSEFSLKSDVLLDEVRAGGRINLIIGRGLTGKARESLGLPTSTLFRTPAAPADT
                     GKGFTLAQKMVGQACGVKGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSAD
                     LVMQSFCHTAAYPKPVDIQTQHTLPDFIMTRGGVSLRAGDGIIHSWLNRMLLPDTVGT
                     GGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVNA
                     IPLYAIKAGLLTVEKKGKKNIFSGRILEVEGLPNLKVEQAFEISDASAERSAAGCTIK
                     LAKEPIIEYLKSNITMLRWMIEQGYGDVRTIERRARKMEDWLANPQLMEADADAEYAA
                     IIDIDLADIKEPILACPNDPDDVKILSEVAGAKIDEVFIGSCMTNIGHFRAAGATQQR
                     PHACQQLGKGEGFHQVIIGAAIQPAHTVLH"
BASE COUNT          463 a          589 c          499 g          411 t
ORIGIN      
        1 ctcattgaat tattagataa cagcgaatta gccgctctgg ccgccgaaga gctcaaacac
       61 acattgctga tgttcgatcg cttccacgat gtagaagaaa aagcccgcgc agggaacact
      121 cacgctaaag ctgtgctgca aagctgggca gatgccgagt ggtttacctc taaacccgct
      181 gtggctgaat ccatcaaatt agccgtgttt aaagtcaccg gcgagaccaa caccgacgat
      241 ctatcccctg ccccagacgc ctggagccgc ccagatatcc cactgcacgc cctggccatg
      301 tacaaaatgg cccgcgatgg cctgacacct gatgaacaag gcgcaaaagg tccgttaaaa
      361 caaatcgaag ccattaaagc caagggaatt cctgttgctt tcgtgggcga tgtggtcggc
      421 accggttctt cgcgcaaatc cgccactaac tccgtattgt ggttctttgg cgatgacatg
      481 cccggcgtgc ctaacaaacg caccggcggt gtgtgtatcg gcagcaaagt ggcaccgatt
      541 ttttacaaca ccatggaaga tgccggcgct ttagtttttg aagccccagt ggataaaatc
      601 gccatgggcg acatcatcga agtgcgccct tacgacggca aaattctctc tgaaagcggc
      661 gcgctactct ccgagttcag cctcaagtcc gacgtgttat tggacgaggt gcgcgccggc
      721 ggccgtatca acctaatcat cggtcgcggc cttaccggta aagcgcgcga atccttgggc
      781 ctgcccacct ccactttatt ccgcacacca gcggcccctg ccgataccgg caaaggtttc
      841 accctggcgc aaaaaatggt tggccaggcc tgcggcgtga aaggtattcg cccgggcact
      901 tactgcgaac cgaaaatgac cacagtcggc agccaggaca ccaccggccc catgacccgc
      961 gacgagctca aagacctcgc ctgtttgggt ttctcggcgg atttggtgat gcagtccttc
     1021 tgccacaccg ccgcctaccc caaacctgtg gacattcaaa cccaacacac cctgccggac
     1081 ttcatcatga cccgcggcgg agtttctctg cgcgccggtg acggcattat tcactcttgg
     1141 ttaaaccgca tgttgttgcc cgacaccgtc ggcaccggcg gcgactcaca cacccgcttt
     1201 ccaattggta tctccttccc agctggctca ggcttagtgg ccttcgccgc cgccaccggt
     1261 gtaatgcctt tggatatgcc tgaatcggtg ctggtgcgtt ttaaaggtga aatgcaaccg
     1321 ggcattaccc tgcgcgattt ggtgaacgcc attccgctct acgctatcaa agctggcctg
     1381 ttaaccgtgg aaaagaaagg caagaaaaac attttctccg gccgcatttt agaagttgaa
     1441 ggcctgccca atttaaaagt ggaacaggcc tttgaaattt ccgacgcctc cgctgagcgc
     1501 tccgccgccg gctgtaccat taaactggcc aaagaaccca tcatcgaata tttaaaatcc
     1561 aacatcacta tgctgcgttg gatgatcgag cagggttatg gcgatgtacg caccattgag
     1621 cgccgcgcgc gcaaaatgga agactggttg gctaatccac aattaatgga agcggacgca
     1681 gatgccgaat acgccgccat tattgatatt gaccttgccg atattaaaga acctatcctc
     1741 gcctgcccga acgatccgga tgatgtgaaa atcctcagcg aagtggccgg tgcgaaaatt
     1801 gacgaagtgt ttatcggttc ctgtatgacc aacataggtc acttccgcgc cgccggagcg
     1861 acgcagcagc gcccgcacgc gtgccagcag ctcggcaaag gcgaagggtt tcaccaggta
     1921 atcatcggcg ccgccatcca gcccgcgcac acggtccttc ac
//
LOCUS       DAOQGS010000720         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_40360_length_1954_cov_5.250132, whole genome shotgun sequence.
ACCESSION   DAOQGS010000720 DAOQGS010000000
VERSION     DAOQGS010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40360_length_1954_cov_5.250132"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..643
                     /gene="rlmJ"
                     /locus_tag="PKZ52_18130"
                     /pseudo
     CDS             68..643
                     /gene="rlmJ"
                     /locus_tag="PKZ52_18130"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
     gene            complement(837..1598)
                     /locus_tag="PKZ52_18135"
     CDS             complement(837..1598)
                     /locus_tag="PKZ52_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG61746.1"
                     /translation="MNLHGIRAIYLFEMARTFRTIAQSIAAPVLSTALYFIVFGTAIG
                     SRMGTIDSVSYGAFIIPGLVMLSLLSESISNASFGIYMPKWAGTIYELLSAPVSWIEV
                     LIGYVGAAASKSIMLGLLILITARVFIPYEILHPVWALAFLVLTAVTFSLFGFIIGLW
                     ADNFQKLQVIPLLIVTPLTFLGGAFYSISMLPPLWQKITLFNPVVYLISGFRWSFYGV
                     ADVHIAVSFGMTLGFLAICLGFVWWIFKTGYKLRK"
     gene            complement(1595..>1954)
                     /locus_tag="PKZ52_18140"
     CDS             complement(1595..>1954)
                     /locus_tag="PKZ52_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HNG61747.1"
                     /translation="IEEAEDMADRIGVIRKGELIVVENKDVLMRKLGKKQLSLNLRQP
                     INSIPERLKDWPLELADGGTTLVYRFDSQTEETGIALLLRTLGEEGIDFKDLRSSESS
                     LEDIFVNLVQAPKEAQL"
BASE COUNT          532 a          496 c          468 g          458 t
ORIGIN      
        1 ctggcagcgg ccgaacctgc cgccgggcct tgcccgctgg cagcactagg tacaggcctt
       61 taacggcggc gtaagtttaa gcgcctaccc cggctccccc tggttcgccc aacaaatcct
      121 gcgcccctac gaccgcctag aactgtgcga gctgcacccg caggacataa cactgctgcg
      181 ccgccaaatg gcgggtgaca aacgggtggc ctgccatttt gaaaatggct acaaacaggc
      241 cttagcccta gttccgccgg ttgaacggcg cggcctaatc cttattgacc cctcctatga
      301 agtcaaaacc gaatacgctc aggtagtaag tgaagttaaa agcctgcacc ggcgctttgc
      361 caccggcgtg tatttaattt ggtacccggt aattgaagcg cgctttgtcg agcaactggt
      421 gcgcggccta aaaaattccg gtatcaaacg catcaaccaa atcgaatggg gcctgggtgc
      481 tggttttaat gaaggcaagg gcatgaatgc caccggccta ctcaccatca acgccccctt
      541 tacccttatg ccctgtttag aagaaagcct caattacgcc ttgcctattt tggcgcctag
      601 cggcgaggga tatgtgaagg tggagacttt ggtgggggag taggtttttt tattggaata
      661 aaaatgagat tggggtaggg cgcactttag tgcgccagcg ggcacctagc ctgtgtgcaa
      721 aataaaccca tattgaaaag ccgatatttt tgtttggacg caagattatt tacgctcaga
      781 cgctgctaac gtcagtcatc tctttgccct gcacgtaatc cgccttacga gtttatttat
      841 tttctaagct tatacccagt cttaaatatc caccaaacaa agcctagaca aatagccaaa
      901 aatccgagcg tcataccaaa gctcactgcg atatgcacat cggctacacc gtaaaaactc
      961 cagcggaagc cactgatcaa ataaacaacc gggttaaata gggtaatttt ttgccaaagt
     1021 ggcggcaaca tgctgatgga gtagaaggca ccgcctaaaa aggttaaggg cgtaacaatt
     1081 aataacggaa tcacctgcag cttttgaaag ttatcggccc ataagccaat aatgaagcca
     1141 aaaagactaa aggtgacggc agtgaggact aaaaatgcta atgcccaaac cgggtgtagt
     1201 atttcgtaag gtataaagac ccgcgcggta attaatatta ataaacccag cataatggat
     1261 ttactggcgg cggcccccac atagcctatt aatacttcga tccaggatac aggcgccgat
     1321 aataattcgt aaatcgtgcc cgcccacttg ggcatataga tgccaaaaga agcattggaa
     1381 atactttcgc tcagtaacga caacatgacc aggcccggaa taataaatgc cccatagctt
     1441 acgctgtcga tggtgcccat gcgtgagcct atggcggtgc caaacacaat aaaatacagc
     1501 gcagtggaga gcacgggggc ggcaatgctt tgggcaatag tgcgaaaggt gcgggccatt
     1561 tcaaacaggt aaattgcgcg aatgccatgc aggttcatag ctgtgcctcc tttggagcct
     1621 ggaccaggtt gacaaaaata tcttccaggg agctttcgct tgagcgcagg tctttaaaat
     1681 caataccctc ttcacccagg gtgcgcagta aaagggcgat gccggtttct tccgtttgcg
     1741 aatcaaagcg atacaccaag gtggtgccgc catcggccag ctccagcggc caatctttta
     1801 agcgctcagg aatggaatta atcggctggc gtagatttaa ggacaattgt tttttgccca
     1861 gtttgcgcat taatacgtct ttattttcta ccacaattaa ttcccctttt ctgatcacac
     1921 caatacggtc cgccatatct tcagcttctt caat
//
LOCUS       DAOQGS010000721         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_40760_length_1944_cov_3.429857, whole genome shotgun sequence.
ACCESSION   DAOQGS010000721 DAOQGS010000000
VERSION     DAOQGS010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40760_length_1944_cov_3.429857"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1047)
                     /gene="ruvB"
                     /locus_tag="PKZ52_18145"
     CDS             complement(79..1047)
                     /gene="ruvB"
                     /locus_tag="PKZ52_18145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNG61748.1"
                     /translation="MIEPDRLIQATASAKDEQFDRAIRPKTLQDYIGQPAVREQMEIF
                     IGAARKRGEPLDHTLVFGPPGLGKTTLANIIAAEMGVALKSTSGPVLEKAGDLAAIMT
                     NLDPGDVLFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVQRLEFYSVADLATIVARSARLLGVPVDTDGALEIAKRARG
                     TPRIANRLLRRVRDFARENPELAVWYDRFGLFDVYGSPWFAAVYLLLFVSLVGCIVPR
                     IRVYVRALRTPPPRTPRNLGRLPASVAGTTRPAAFETSTVSDSITR"
     gene            complement(1076..1696)
                     /gene="ruvA"
                     /locus_tag="PKZ52_18150"
     CDS             complement(1076..1696)
                     /gene="ruvA"
                     /locus_tag="PKZ52_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNG61749.1"
                     /translation="MIGRLTGRLVEKQPPHLLIDVQGVGYELLAPMTTFYQLPGLGES
                     LSLYTHLSISEAAHQLFGFASSADRSFFRLLIKVTGVGPKMALAILSAMDAQECAQCL
                     LQNNLIALVRIPGVGKKTAERLVIEMRDRLKDWAPQASASPQAAQDLPAAQVLNAMVA
                     EAESALVALGYKPAEAAKAIAAAMKQQELTRTEDVIRAVLRAMSPT"
     gene            complement(1693..>1944)
                     /locus_tag="PKZ52_18155"
     CDS             complement(1693..>1944)
                     /locus_tag="PKZ52_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNG61750.1"
                     /translation="LAAACAGLSVHEYEARKVKQAVVGTGAADKTQVQHMVKSLLKLA
                     ASPQEDAADALAVAICHANSFHLTARVELSGRFSRGRMR"
BASE COUNT          379 a          587 c          611 g          367 t
ORIGIN      
        1 gcgcagcgcc accacgctgg gccaccagcg cgagggcacc ggcgtgctgg tgcgcgaggg
       61 ctacgtgctc accatcggct acctcgtgat cgagtccgag accgtcgagg tctcgaacgc
      121 ggcggggcgc gtcgtgccgg ccacgctcgc ggggaggcgg cccaggttgc ggggggtgcg
      181 gggcggcggg gtgcgcagcg cccggacgta caccctgatg cgggggacga tgcaccccac
      241 caacgacacg aacagcagca ggtagacggc cgcgaaccac ggcgagccgt acacgtcgaa
      301 caacccgaac cggtcgtacc agacggccag ttcggggttc tcgcgggcga agtcgcgcac
      361 ccgccgcaac aggcgattgg ctatgcgcgg agtgcccctg gcgcgcttgg cgatttctaa
      421 ggcgccgtcg gtgtctacgg gaacccccaa caaacgggcc gagcgcgcca cgatagtggc
      481 cagatccgcc acggaataaa actccaaacg ctgcactatg ccaaatcgat cgcgcagcgg
      541 agacgtcaac agtcccgccc gcgtggtggc tcccaccagg gtaaagggtg gcagatccag
      601 tttgatggag cgcgctgcag ggccttcgcc gatcatgatg tccaactgat aatcttccat
      661 ggccggatag aggatttcct ccaccaccgg gcttaggcgg tggatctcgt cgataaagag
      721 cacatcgccc ggatcgaggt tggtcataat cgcggctaaa tcacccgctt tttccagcac
      781 agggccggag gtggatttaa gagccacccc catttcggcg gcgatgatat tggccagggt
      841 tgttttaccg agtcccggcg ggccgaacac cagggtgtga tccagcggtt cgccacgctt
      901 gcgcgcagcg ccgataaaaa tttccatctg ctcgcgcacc gcaggttgac ctatgtagtc
      961 ctgcagtgtt ttcggccgga tggcgcggtc aaattgttcg tctttggcgg aggcggtggc
     1021 ctgaataagg cggtcgggtt caatcacagg gcgggccttg cttgatcagc aattgttagg
     1081 tgggcgacat ggcgcgcagc acagcgcgga ttacatcctc tgtgcgcgta agctcctgct
     1141 gcttcatggc tgcggcaatc gccttggccg cttcggcagg cttataaccc agcgccacca
     1201 gggcactttc tgcctcggcc accatggcgt tgaggacctg ggcagcgggc aaatcctgcg
     1261 ccgcctgggg gcttgcagag gcctgcggcg cccaatcttt gaggcgatcg cgcatctcaa
     1321 tcaccaaacg ctcggcggtt tttttgccga caccgggaat gcgcaccagg gctattaagt
     1381 tgttttgcag taggcattga gcgcattctt gggcgtccat ggcggagagt atggccaggg
     1441 ccattttggg gccaacaccg gttaccttaa taagcaagcg gaagaaactg cggtcagcgc
     1501 tgctggcgaa gccaaataac tgatgggccg cttcgctgat ggagagatgg gtatagaggc
     1561 ttagcgactc gcccaaaccg ggcagctggt aaaacgtggt cataggcgcg agcaattcat
     1621 agcccacccc ctgcacgtcg atcaacaaat ggggcggttg tttctctacc aacctaccgg
     1681 ttaaacgccc tatcaccgca tacgccctcg gctaaaacgc ccggagagtt caacgcgggc
     1741 tgttaaatgg aaactattgg cgtggcaaat ggccaccgcc agcgcatcgg ccgcatcctc
     1801 ctggggggat gcggccagtt ttaaaagact ttttaccatg tgttgcacct gggttttatc
     1861 cgcagccccc gtgcctacca ccgcctgttt taccttacgc gcttcgtatt catgcacgct
     1921 aaggcctgcg caggcggctg ccag
//
LOCUS       DAOQGS010000722         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_40781_length_1943_cov_3.890890, whole genome shotgun sequence.
ACCESSION   DAOQGS010000722 DAOQGS010000000
VERSION     DAOQGS010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40781_length_1943_cov_3.890890"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKZ52_18160"
     CDS             <1..467
                     /locus_tag="PKZ52_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG61751.1"
                     /translation="PFYSGHLRGRCTTNVEVCVTAFDFALENWPLVAAFGGLIVAYFV
                     VNSAAGGKSISVHEVTRLVNQGNALLLDVREANEFKAGHITGAINIPFGAVSARVEEL
                     EKHRGKHIILVDKIGQHAGEVAKQLKAQGFDVVRLGGGMSEWTHQSLPVVKK"
     gene            483..743
                     /gene="grxC"
                     /locus_tag="PKZ52_18165"
     CDS             483..743
                     /gene="grxC"
                     /locus_tag="PKZ52_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNG61752.1"
                     /translation="MSIKVYTRRTCSYCVAAKQLLRSKGVEFEEIGLDNDPQLLQEVM
                     TKARQRTVPQIWIGSRHVGGFTDMLALDRAGELDGLLANPPA"
     gene            806..1300
                     /gene="secB"
                     /locus_tag="PKZ52_18170"
     CDS             806..1300
                     /gene="secB"
                     /locus_tag="PKZ52_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNG61753.1"
                     /translation="MTDEINNAESSADESQAPQFALQRIYIKDVSFETPMGVEAFRQQ
                     WQPKVGQELGTAANKIDEDLYEVILKITINIQLGDKTAFLVEVQQAGLFMVANIEGPQ
                     LAHLLNTACPQILFPYAREAIDNLCLKGSFPALALPPINFDMLFARAIAQAQAEAEGA
                     GQDA"
     gene            1495..>1943
                     /locus_tag="PKZ52_18175"
     CDS             1495..>1943
                     /locus_tag="PKZ52_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNG61754.1"
                     /translation="MSDAPQAPYLNRRTFAIISHPDAGKTTITEKLLLFGNAIQIAGS
                     VKGKRGPHAKSDWMTMEQERGISVTSSVMQFPYKGRTVNLLDTPGHEDFSEDTYRTLT
                     AVDSVLMVIDGAKGVEDRTIKLMEVCRLRDTPILSFINKMDRESRDPV"
BASE COUNT          463 a          483 c          499 g          498 t
ORIGIN      
        1 cgccttttta cagcggccat ttgcggggcc gctgcaccac taatgttgag gtatgtgtga
       61 ctgcatttga ttttgcactg gagaattggc ctttggtggc cgcgtttggt gggctgatag
      121 tggcgtattt tgttgtcaac tcggcggctg gcggaaaaag tatttccgtg catgaagtga
      181 cccgtctggt caaccagggc aatgctttgc tgttagatgt gcgcgaggcc aatgaattca
      241 aagcgggcca tattactggc gctatcaaca taccttttgg cgccgtgagc gcgcgggtgg
      301 aggagctgga gaaacaccgc ggcaaacaca tcatactggt ggataaaatc ggccagcacg
      361 caggtgaagt cgccaaacaa cttaaagccc agggttttga tgtggtgcgt ttaggcggtg
      421 gcatgagcga gtggacccat caatccctgc cagtggtaaa aaaatagcca tagaggtttg
      481 ctatgagtat taaggtttat acccgccgca cctgtagcta ttgcgtagcc gccaaacaat
      541 tgctgcgcag taaaggcgtg gagtttgaag agattggctt ggataacgac ccgcagttgc
      601 tacaagaggt gatgactaaa gcccgccagc gcacagtgcc gcaaatttgg atcggcagcc
      661 gccatgtggg tggttttacc gatatgctgg cgcttgatcg cgcgggcgaa ttggatgggc
      721 tgttagccaa tccaccggct taaatgttct attctttgtc ccaacacctg tttaattctt
      781 taacctaact ttttaaggcc tttctatgac cgacgaaatc aacaacgctg agagctctgc
      841 cgacgaatcc caggcccccc aatttgccct gcagcgcatt tatatcaagg acgtttcctt
      901 tgaaaccccc atgggtgtgg aagcgtttcg tcaacagtgg cagcctaaag tcggtcagga
      961 attgggtact gccgccaata aaattgatga agacctctat gaggtgattt taaaaatcac
     1021 catcaatatt cagctcggcg acaaaactgc cttcttggtg gaagtgcaac aggccggttt
     1081 atttatggtg gccaacatcg aaggtccaca attagcccac ttgttaaaca ctgcctgccc
     1141 acaaatttta ttcccctacg cccgcgaagc catcgacaac ctgtgcttaa aaggcagttt
     1201 cccagccctt gctttgccgc cgattaattt tgatatgttg ttcgcccgcg cgattgccca
     1261 ggctcaggct gaagccgaag gcgcaggtca agacgcataa ccattttgcg gcagcttttg
     1321 ggttgccgct gtttactttt aataaaaaac ctgccgaggc gggttttttt attggaagcg
     1381 atttaaccta aaaattgccg ttatactctg ttgcagtttt tgggtttaat ttcataaccc
     1441 ccgcttgccg agtcggttag taaattattc tttacgttta tttttgagcc cgccatgagt
     1501 gacgcccccc aggcacccta tttaaatcgc cgcaccttcg ccattatcag tcacccggat
     1561 gccggcaaaa ccactattac cgaaaaatta ctgttgttcg gcaacgccat tcaaattgcc
     1621 ggttcagtaa aaggcaagcg cggcccccac gccaaatccg actggatgac catggagcag
     1681 gagcggggaa tttccgttac ctcatcggtg atgcaatttc cctacaaggg ccgcacggtg
     1741 aatctgttgg atactccggg ccacgaggat ttctcagagg atacctaccg caccctaacg
     1801 gcggtggatt cggttttaat ggtgattgac ggcgccaagg gtgttgagga tcgcaccatt
     1861 aaattaatgg aagtctgccg cctgcgggat acgcctattt tgtccttcat taacaaaatg
     1921 gaccgggaaa gccgcgaccc ggt
//
LOCUS       DAOQGS010000723         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41003_length_1937_cov_4.196599, whole genome shotgun sequence.
ACCESSION   DAOQGS010000723 DAOQGS010000000
VERSION     DAOQGS010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41003_length_1937_cov_4.196599"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKZ52_18180"
     CDS             <1..464
                     /locus_tag="PKZ52_18180"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNG61755.1"
                     /translation="PDQLLDKLEALVAQNRHNEKALAALKGKLAAASANEWVNDAIAA
                     GGVQLLVKQLETGDGDSLLQLVDQLKNKLGEAIVLLAGVEDGKVTLVAGASKAASAKV
                     KAGDLVKLVAELLGGKGGGRPDMARGAGSDNGQVPQVLEKALAWLQAQLGN"
     gene            587..1807
                     /locus_tag="PKZ52_18185"
     CDS             587..1807
                     /locus_tag="PKZ52_18185"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNG61756.1"
                     /translation="MSLIVQKFGGTSVGTTDRIKNVADKIIKFRQQGHDLVVVVSAMS
                     GETNRLIGLAKDIQAEPDGRELDVLVSTGEQVTIALLCMALKERGQDARSYTGSQVRI
                     LTDNAYTKARIQEIDVERMRADLDEGKVVVVAGFQGVDEEGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRVADRARRLDRITFEEMLEMASLGSKVLQIRSVEF
                     AGKYNVPLRVLSTFADGPGTLITIDEEFPTMEQPVVSGIAFNRDEAKLTIRGVPDRPG
                     VASLVLAPIGDANVEVDVIVQNLSADNTTDITFTVNRGEMSKAKGILDSVAAEIGARE
                     VVADNTVVKVSVVGVGMRSHAGVAAKMFKTLAQENINIQMITTSEIKISVIIEEKYLE
                     LAVRALHTAFGLDK"
BASE COUNT          478 a          471 c          553 g          435 t
ORIGIN      
        1 gccccgatca gctgcttgat aagctggaag ccctggtggc ccaaaaccgc cacaacgaaa
       61 aagcccttgc cgcgttaaaa ggcaagcttg cggctgcgtc tgccaatgag tgggtgaatg
      121 atgccattgc cgccgggggc gtgcagttgt tggtgaagca gttggaaacc ggcgacggcg
      181 acagcttgtt acagctggtg gaccaactta agaacaaact aggtgaagcc atcgtgctac
      241 tagccggtgt agaggacgga aaggtgaccc tggttgctgg ggcgagcaag gcggccagcg
      301 ccaaggtgaa agcgggtgac ctggtgaaat tggtcgccga actattgggc ggcaagggcg
      361 gtgggcgccc ggacatggca cggggtgcgg gcagtgacaa cggccaagtg ccccaggtgc
      421 tggagaaagc gctggcctgg ttgcaggcgc aattgggaaa ttagacgaaa ggagaagaat
      481 tcccatagat gttgttaaca atccacggca attagtgttt aattggcgcc ctttttcgcc
      541 tcccatatcg agcggcgatt ggttttaatt ggagacacgc tcagccatga gtttaatagt
      601 gcaaaagttt ggtggtacct cggtaggcac aaccgaccgc ataaaaaatg ttgcggacaa
      661 aattattaaa ttccgccagc agggccacga tttagtggtg gtggtctccg ccatgagcgg
      721 tgagaccaac cgtttaattg gccttgctaa agatattcaa gcggagccgg atggccgtga
      781 gcttgatgtt ctggtctcta ccggtgaaca agtgactatc gcgcttttat gtatggccct
      841 caaagagcgc ggccaggatg cccgttcgta caccggttca caggtgcgta ttctcaccga
      901 taacgcctac accaaagccc gcatccaaga aatcgacgtg gagcgcatgc gcgcggacct
      961 cgacgagggt aaagtagttg tggtggcagg gttccaaggt gttgatgaag agggcaatat
     1021 cactacctta ggccgcggcg gttctgatac cacgggtgtc gccctggccg cggcacttaa
     1081 agccgacgaa tgtcagatct ataccgatgt tgacggtgta tacaccaccg acccacgggt
     1141 ggctgatcgc gcccgcaggc tcgatcgcat tacctttgaa gaaatgcttg aaatggccag
     1201 cctcggctcc aaagtgttgc aaatccgctc ggtggaattt gctggcaaat acaacgtgcc
     1261 gttgcgcgtc ctctcgacgt ttgccgacgg ccctggaaca ctcattacta ttgacgagga
     1321 attccccacc atggaacaac ctgttgtttc tggcatcgca tttaaccgcg atgaagccaa
     1381 attaaccatt cgcggtgtgc cggacaggcc gggtgtcgcc tccttggtat tagcacccat
     1441 aggcgatgcg aatgttgagg tcgatgtgat tgtgcagaac ctctctgctg ataacaccac
     1501 cgatattacg tttaccgtta atcgcggtga aatgagcaaa gccaaaggaa tacttgattc
     1561 cgtagcggct gaaataggcg ctagagaagt tgtagctgat aatactgtgg ttaaggtgtc
     1621 tgtggtgggc gttggtatgc gttcacatgc tggcgttgcc gccaaaatgt tcaaaaccct
     1681 ggcccaagaa aacattaata ttcaaatgat taccacatct gaaatcaaga tttccgtgat
     1741 catcgaagag aagtatctgg agctggctgt acgtgcattg cacacagcat ttggtctgga
     1801 taaataacat caacccattg tccctgaaag gcttgccttt tgggttgccc tcgggcatgg
     1861 cagatcttgc gggttccaaa ccaagagcat aatcctttta tgcatctata ctaaaaaccg
     1921 gaacccggaa cgcaagg
//
LOCUS       DAOQGS010000724         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41035_length_1936_cov_5.659224, whole genome shotgun sequence.
ACCESSION   DAOQGS010000724 DAOQGS010000000
VERSION     DAOQGS010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41035_length_1936_cov_5.659224"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKZ52_18190"
     CDS             complement(<1..531)
                     /locus_tag="PKZ52_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61757.1"
                     /translation="MAKKILLGVYLTLSAVHIQAGEIPSFIKTEHIASLKSYLIARPN
                     LILMPESACTCDTSLAQLRKEIPNFQPYYAIGDFNRDKIDDFAIALSDTNKPSGTEPT
                     MTVVIFHGPFNKTPKKAIEVVKDYAIKRPKEVISAFNSRLEAGHLIPGRLDLGPAIFG
                     SDDIHMIIYDTKTQKYR"
     gene            complement(887..1330)
                     /locus_tag="PKZ52_18195"
     CDS             complement(887..1330)
                     /locus_tag="PKZ52_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG61758.1"
                     /translation="MHLSIATAEDIPALADLLAILFCQEAEFTPNPPLQIAALNKIIA
                     NPTLGEIIIARINDEAVGMVNLLYTCSTALGGTVGLLEDMVIKPGWRRQNLGTHLLEY
                     AIDRARSRGCLRITLLTDSNNTEAQRFYHRQGFTASTMLPMRLIL"
BASE COUNT          482 a          398 c          497 g          559 t
ORIGIN      
        1 gcggtatttt tgtgtcttgg tatcgtaaat gatcatgtgt atatcgtctg agccaaatat
       61 tgctgggcca aggtcaagtc gtcccggtat gaggtggccc gcttcaaggc gcgagttaaa
      121 agcggatata acctccttag ggcgtttaat tgcataatcc tttacaactt caatagcttt
      181 ctttggagtt ttattaaatg ggccgtggaa aataactact gtcattgtgg gttcggtgcc
      241 ggacggtttg tttgtgtctg ataaggcgat ggcaaaatcg tcaattttgt cgcggttaaa
      301 gtcaccgatt gcatagtatg gttggaaatt tggaatctct tttctaagtt gggctaatga
      361 tgtgtcacag gtgcatgctg attcgggcat taagataagg tttgggcgcg cgattaagta
      421 gctttttagg ctggcgatgt gctcggtctt aataaaagat ggtatttctc ccgcctggat
      481 atgcactgcc gatagggtta ggtaaactcc caagaggatt ttttttgcca tggtttatct
      541 cctgaaaatc aatttcgtca tttgcataat gaggtattga gggctggtgt ttgcttttgc
      601 gtaattgtgc agcaccattt ttaacccgcg cgcgatgggt ataggcgggt gcgtgtttgg
      661 gttagttgag cagtttaaat attgtccgca gcaatgaata cagattactt cagacacgcc
      721 gtgaatacat ccgtgtaggc tcgtcaaaaa gatccctctt tttgacggtc tgaaataacc
      781 tgtattcatt tctgctgcac cgcgctcaaa ttataccgat tggcttaatt ttacataaac
      841 ttccactaat ggtgatggcg aaaagcaggg cttaatagct atgttcttac aatattaaac
      901 gcatgggcag catggtggag gcggtaaaac cctgacggtg ataaaaacgc tgggcctcgg
      961 tgttgttgct gtcagtgagt agggtaatgc gtaggcagcc tctgctgcgg gcgcggtcta
     1021 tggcgtattc taacaaatgg gtacctaaat tctgccgacg ccagcccggt tttatcacca
     1081 tgtcttccaa caggccgacg gtgccgccta aagcggtgga gcaggtgtag agtaaattca
     1141 ccatgccgac ggcttcgtca tttatgcgcg caataataat ttcgccaagg gttggattgg
     1201 caataatttt attaagggcg gcgatttgta gtgggggatt gggggtgaat tctgcttctt
     1261 ggcaaaataa gatggctaaa agatcggcca gggccggtat atcctcagct gtggcgattg
     1321 ataaatgcat agtaattatt gagtaccttt ttagtattgc gggttagtgt aactggacct
     1381 gatgtttgcg ccgactcgaa atcgggcgag ccttcactac tcaacccgca gggatgaata
     1441 caggttattg cagacacgct gtaaatacat ccgtgtaggc tcgtcaacaa ggtccctctt
     1501 tttgacggtc tgcaataacc tgtagtcaca actgctatac cgcgaatcga ttctgccgct
     1561 gggttaaatt cccctcaatt tgccgcgcat atggcgaggg caataataaa ctgtgctcga
     1621 tagtgaccgc atatttaaat ctggctgcta actaaaatta aaatggttta acccggccgc
     1681 ttttaatacc gggaaaaagc tgttgcgctc gggccgagtg gtaatgtcat taagttaagg
     1741 gttttgaggt gaatctttac ccgtaggttg gaagagcgta gcgacttccg acatccatcc
     1801 gccagtacaa actacaccag tagaaactac accagtacaa actacaccag tacaaactac
     1861 aagtgcggca aggactctgt gggatgtcgg aagtcgctgc gctcttccat atatggactc
     1921 ccccaatatt gccagt
//
LOCUS       DAOQGS010000725         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41281_length_1930_cov_3.597333, whole genome shotgun sequence.
ACCESSION   DAOQGS010000725 DAOQGS010000000
VERSION     DAOQGS010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41281_length_1930_cov_3.597333"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKZ52_18200"
     CDS             complement(<1..460)
                     /locus_tag="PKZ52_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61759.1"
                     /translation="MTEVSSPFAKALLRELEHHTFSVVLPGAAGVLAAEPGVLVDTRA
                     YAGIEVAHEIDTVPDSALPIIHISSGEVFQATGTAVGFHEQDAPNGSSPRALALIAAE
                     ARALERSKTLVLRLPLLVDVRVGDWFSGLLERLSREAVLSVSETARLDPVC"
     gene            559..>1930
                     /locus_tag="PKZ52_18205"
     CDS             559..>1930
                     /locus_tag="PKZ52_18205"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNG61760.1"
                     /translation="MKIIVWFTQDLRLSDHPALNAALKDGDEVYCIYVFDPENSQGPQ
                     GAAQWWLHHSLNKLAEDLAAKGGQLILRSGNSSEQLLKLAAELQAQAIYMTRSYEPYL
                     SHEQTKLYEQMTRQGGTAKRFAGFLLLEPDSILNKQAKPFQVFTPFYRHSRELIEHKI
                     KAAPKAQYTGLTAKPTKLKSEALNQWRLLPSKPNWAREFADYFTPGEAGALAALNRAL
                     ADAVENYAERRDFPALPGTSCLSAHLHFGEISPRYVLQQAINHFGGEAAAPFTRQLFW
                     REFNYYLLHHYPHMRTNAFKAKFDSFPWRPNKPYLKAWQQGKTGYPIVDAGMRQLWRT
                     GWMHNRVRMICASFLTKHLQIHWQEGEAWFWDTLLDADKANNIGGWQWVAGCGADAAP
                     YFRIFNPIVQGEKFDPLGDYVRTWVPELSRLPPAFIHKPWLAPAGLLAQCGIKLGTDY
                     PSPLVNH"
BASE COUNT          469 a          601 c          491 g          369 t
ORIGIN      
        1 tacaaaccgg gtcgagccgc gcagtctcag aaacacttaa caccgcctcc ctactcaagc
       61 gctccaacag gccgctaaac caatcgccaa cacgcacatc caccaacagg ggtaagcgca
      121 gcacgagggt tttgctgcgc tccagagccc gcgcttccgc cgcgataagt gcaagggcgc
      181 ggggcgagct gccattgggg gcgtcctgct cgtgaaaacc caccgcagta cccgtagcct
      241 gaaacacctc gccacttgag atatgaatga tcggcagggc actgtccggc acagtgtcga
      301 tttcatgcgc aacctcaatg cctgcataag ctcgggtatc caccaagacg ccgggctccg
      361 ccgccagcac accggcggcg ccagggagca ccaccgaaaa ggtgtgatgt tccagctccc
      421 gcagcagcgc cttagcaaag ggcgagctta cttctgtcaa gactatcttg aaggccaaac
      481 aacacctacc ttacgcaatt gtggggatcc gattctactg actctgcgta gcataccaac
      541 agacccaaag agcccacgat gaaaataatt gtttggttta cccaggactt acgcctcagc
      601 gaccaccccg ccctcaatgc cgccctcaaa gacggcgatg aagtctactg catatacgtc
      661 ttcgacccag aaaatagcca aggcccccaa ggggccgccc agtggtggct gcaccacagc
      721 ctaaataagc ttgccgagga cttagcggcc aaaggcggcc agttaattct gcgcagcggc
      781 aacagcagcg aacagctgct taaactcgcc gcagagctgc aggcgcaggc gatctacatg
      841 actcgcagct acgagcctta cctcagccat gagcaaacta agctctacga gcagatgact
      901 cgacagggcg gcaccgccaa acgctttgcc ggttttttac tgttggagcc cgacagcatc
      961 ctcaacaaac aggctaaacc cttccaggtg tttacaccct tttaccgcca cagtcgcgag
     1021 ctgatcgaac acaaaattaa agcggccccc aaagcccagt acacaggcct caccgccaaa
     1081 cccaccaagc tcaaaagtga agccttaaac caatggcgct tgctgccgag caaacccaat
     1141 tgggcccgtg aatttgccga ctacttcacg ccgggggagg ctggcgccct ggcagctttg
     1201 aatagggctt tggcagatgc agtagaaaat tatgccgagc ggcgagactt ccccgcattg
     1261 ccaggcacca gctgcctctc ggcccatttg cacttcggcg aaatcagccc tcgttatgta
     1321 ttacagcagg cgattaatca ttttggtggg gaagcagcgg cgccttttac ccggcaatta
     1381 ttttggcggg aatttaatta ttacctgctg caccactacc cccatatgcg caccaacgcc
     1441 tttaaagcca aattcgacag cttcccctgg cggcccaata agccttattt aaaagcctgg
     1501 cagcagggca aaacgggtta ccccatagtg gatgccggta tgcggcaact gtggcgcacc
     1561 ggctggatgc acaaccgggt gcgaatgatc tgcgcctctt ttttaactaa acacctgcaa
     1621 attcattggc aagaaggcga ggcttggttc tgggatacgc tcctagatgc cgacaaggcc
     1681 aataatatcg gcggctggca atgggtggct ggctgcggcg ccgacgccgc gccttatttc
     1741 agaattttta accccattgt gcagggggaa aaattcgacc ccttggggga ttacgtgcgt
     1801 acctgggtgc cggaattatc ccgcctgccg cccgcgttta tccataaacc ctggctggca
     1861 ccggccgggc tgttggccca atgcgggatt aaactcggca ctgattaccc cagcccactg
     1921 gtcaatcacg
//
LOCUS       DAOQGS010000726         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41312_length_1929_cov_3.674493, whole genome shotgun sequence.
ACCESSION   DAOQGS010000726 DAOQGS010000000
VERSION     DAOQGS010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41312_length_1929_cov_3.674493"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="pyrD"
                     /locus_tag="PKZ52_18210"
     CDS             <1..715
                     /gene="pyrD"
                     /locus_tag="PKZ52_18210"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HNG61761.1"
                     /translation="GFNNKGVAHLVSQVAQKRQYPGILGINIGKNKHTADEDALKDYE
                     HCLAAVYPYADYITVNISSPNTPGLRALQYGDTLSALLDGLKLRQQRLSLEHGKQVPL
                     VVKIAPDMSDDEITDFAQRIINSGMDGIIATNTTLSREGIEGVKHADEAGGLSGAPVK
                     NKSTHTIKVLAQNLQGKLPIIGVGGILNGEDAAEKLRAGASLVQIYSGFIYRGPQLIY
                     EACASFEVFKKEGIKIIKP"
     gene            complement(749..952)
                     /gene="rmf"
                     /locus_tag="PKZ52_18215"
     CDS             complement(749..952)
                     /gene="rmf"
                     /locus_tag="PKZ52_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HNG61762.1"
                     /translation="MKRQKRNPTDRAQARGYQTGASGGSWDMCPFASGEARERWMAGW
                     REAREDHWNGFNSKAQAQKISNF"
     gene            1275..>1929
                     /locus_tag="PKZ52_18220"
     CDS             1275..>1929
                     /locus_tag="PKZ52_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNG61763.1"
                     /translation="MQLFATCPKGLENLLQDELTALGAREARATVAGVYFDADIVQAM
                     RVCLHSRLANRVLLPLGVEDVSSDYDLYSAAYTIDWQNHLRPDCTFAVDFTGTNDAIK
                     NTQFGAMRIKDALVDKLRDHTGTRPNVDRHTPRFRVNARLAKNKVHIGIDLSGESLHR
                     RGYRLEQGVAPLKENLAAAILMRANWPQIAAEGGHLIDPMCGSGTLLIEAALMAGNIA
                     "
BASE COUNT          488 a          493 c          493 g          455 t
ORIGIN      
        1 ggggtttaat aataaaggcg tggcccattt ggtaagccag gtagcgcaaa agcgccaata
       61 ccccggaatt ttggggatta atatcggcaa aaataaacac accgccgatg aagatgccct
      121 caaagattac gagcactgcc tggcggcggt ttacccctat gccgactata taacggtgaa
      181 tatttcctcc cccaatacac ccggcctgcg cgcgctgcaa tacggtgaca ccctcagcgc
      241 tttattagac ggccttaaat tgcgccagca gcgcttgagc ttagagcacg gcaaacaagt
      301 gcctttggtg gtaaaaattg cgccggatat gagcgatgac gaaataacgg attttgccca
      361 gcgcattatt aattccggca tggatggcat tattgccact aataccacat taagccgcga
      421 aggaattgaa ggcgttaaac acgccgatga agccggtggt ttaagcggcg cgccggtaaa
      481 aaataaatcg acccacacga taaaggtgct cgcccaaaac ctgcagggca aattgccgat
      541 tattggtgtg ggcggcattt taaatgggga agacgccgcc gaaaaactgc gtgcaggtgc
      601 tagcctggtg caaatttatt ccgggtttat ttatcgcggc ccgcaattaa tttatgaggc
      661 ctgtgccagt ttcgaagtgt ttaaaaaaga gggtattaaa attattaagc cctaaaagta
      721 gggcttaata ataaggggag ggaagtgatt aaaaattact gattttttgt gcttgagctt
      781 tactgttgaa gccgttccaa tgatcctcac gcgcctcccg ccaaccggcc atccagcgct
      841 cgcgcgcttc gccgctggca aatgggcaca tatcccaaga gccgccgctg gcaccagttt
      901 gatagccacg cgcctgggcg cgatcagtgg ggttgcgttt ttgtcgtttc ataggcattc
      961 acctttagga taaacgggca tggattaggg cagctctgcg cagtgcgcgc tggggttttg
     1021 tagttcagcg cgacactaaa caaagggacc tgtgcagcag cgggtagttc attgcagcca
     1081 gggccatggt actctgcttt tattagccta gacagcccct gctgtagtgc agaaatttcc
     1141 gcattttttg tgacacaatc cgacgaataa ccattaaacc gacagttgtg gcataacaac
     1201 ttttgaatca tcagcttaac cttataaacc accatccttc gcccagtggc ggaagatgct
     1261 aacgagatag aaccatgcaa ttatttgcca cctgccccaa aggccttgaa aacctcctgc
     1321 aagacgaact cactgcctta ggcgcccgcg aggcccgcgc caccgtggcc ggtgtgtatt
     1381 ttgatgcgga tatagtgcaa gccatgcggg tctgcctgca ctcgcgctta gccaaccgcg
     1441 tcttattacc cctgggggta gaggatgtca gcagcgatta cgatttgtat tcagcggcct
     1501 ataccatcga ctggcaaaat catttaaggc cggattgcac ctttgcggtg gatttcaccg
     1561 gcaccaacga cgccattaaa aatacccaat ttggcgccat gcgtattaaa gatgctttag
     1621 tagacaagct gcgtgatcac accggcaccc ggcctaatgt ggaccgccac acaccgcgtt
     1681 ttcgtgttaa tgcccgttta gccaaaaata aagtgcatat aggtatcgat ttatccggcg
     1741 agagtttgca ccggcgcggc taccgtttgg agcagggcgt ggcaccctta aaagaaaacc
     1801 tcgccgccgc cattttaatg cgcgccaatt ggccgcagat tgccgccgag ggtggccatt
     1861 taattgaccc catgtgtggc tccggcactc ttttaattga agcggcctta atggcgggca
     1921 atattgcac
//
LOCUS       DAOQGS010000727         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41345_length_1928_cov_4.252536, whole genome shotgun sequence.
ACCESSION   DAOQGS010000727 DAOQGS010000000
VERSION     DAOQGS010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41345_length_1928_cov_4.252536"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKZ52_18225"
     CDS             <1..410
                     /locus_tag="PKZ52_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61764.1"
                     /translation="PGGVTKIAATLDHCVDVNGDGMITTSTGPADIKPKGQDECVLWF
                     KPIPSPGYTYGVRATAWEGVKADPITCVVPTPRLWFAWMDGNMTAHFMRVDGATGAAK
                     GGDNLQYGLGFAAGVAVCGGGVAGDGSSRRFRA"
     gene            complement(612..1409)
                     /locus_tag="PKZ52_18230"
     CDS             complement(612..1409)
                     /locus_tag="PKZ52_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019605019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG61765.1"
                     /translation="MNPLQPYLEIHQADVFQGSRQVFNALDLTLYYGQHTVILGPNGA
                     GKSTLIKLLTRELYPRVTDNSYVKIDGSERVIIWELRKKIGLVSQDIQAGYDGHIKAQ
                     DVVLSGLFGSVGMHMHQLPSPEQIQAAAEALAQVGLSDCAERYYAHLSTGQQRRLLLA
                     RALIHKPRVLILDEPTNGLDLQGAFALIQLLRRLAQTGVSIVLVTHHLGEIIPEITRV
                     VLLQEGQIVADGDKAEVLTESNLTALYNTPLRLTQADGFYQAYPQSS"
     gene            complement(1416..>1928)
                     /locus_tag="PKZ52_18235"
     CDS             complement(1416..>1928)
                     /locus_tag="PKZ52_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNG61766.1"
                     /translation="DGRVRAPRSGRYNLQPCPFGLVEAADELTRRGFKVLPYCTDDLV
                     LCQKLLDVGCQVLMPWGSPIGTGLGLMNKYNLKTLRERLPNVPLVVDAGLGAPSQACE
                     AMEMGFDAVLLNTAVAKAADPVLMAKAFAAAIAAGRSAYLAGLIVKRQTASPSTPTIG
                     QPFWHQFPKG"
BASE COUNT          409 a          540 c          589 g          390 t
ORIGIN      
        1 accctggggg cgtcaccaag atcgcggcga cgctcgacca ctgcgtcgac gtcaacggcg
       61 acggcatgat cacgacctcg accggcccgg cggacatcaa gccgaagggg caggacgagt
      121 gcgtcctgtg gttcaagccg atcccctcgc cgggttacac ctacggcgtg cgggcgacgg
      181 cgtgggaggg cgtcaaggcc gacccgatca cctgcgtcgt gccgacgccg cgcctgtggt
      241 tcgcgtggat ggacggcaac atgactgccc acttcatgcg cgtcgacggg gcgacgggcg
      301 cagctaaagg tggcgacaat cttcaatacg gccttggctt cgctgctggc gttgctgtct
      361 gcggcggtgg ggttgcaggg gatggaagta gtagaagatt cagagcttaa agggcggtta
      421 aagccggttt tgcagccggt taagcttgcg catagcgcca ggcagatgat gctagaaagt
      481 cgaaccataa aaagacctta acagggcata ggtgagaaag cggccatgga cacgcccagg
      541 aattaaaagt ttgtcacagc atgtgcgcct tgtcagattc cagcgccaat tttgttaggt
      601 aattcgaatg cttaagacga ctggggatag gcttggtaaa acccatcggc ctgggtaagc
      661 cgcaatggag tgttgtacag ggcggttagg ttcgactctg taagtacctc agccttatcc
      721 ccatcagcca ctatctggcc ttcttgcagt aacaccacgc gagtaatttc cgggatgatc
      781 tcgcctaggt ggtgggtcac caaaacaatg cttacccccg tttgcgcaag gcggcgtagc
      841 agctgaatca gcgcgaaggc gccctgtaaa tccaagccgt tggtgggttc atccagaatt
      901 aacacgcggg gcttatgaat cagcgcgcgg gccaataaca ggcgccgctg ctggccggtg
      961 gagagatgcg cgtagtagcg ctcggcgcag tcgctcaagc ccacctgggc gagggcctct
     1021 gctgccgctt gaatctgctc agggcttggc agttgatgca tatgcatgcc caccgagccg
     1081 aacagtccgg agagcaccac atcctgggct tttatgtggc cgtcgtagcc agcctgtata
     1141 tcctgggata ccaggccgat ttttttgcgc agctcccaaa tgatgacccg ttcgctgcca
     1201 tcaatcttta catagctgtt gtcagtcacc cgtgggtaca gctctcgggt gagcaactta
     1261 atcagcgtgc ttttgcccgc accattgggg ccgagtatca ccgtgtgctg gccgtaatag
     1321 agggttaaat ccagggcgtt aaatacctgc cgactgcctt gaaagacatc ggcctggtga
     1381 atttccaggt aaggctgtaa agggttcatg caggactagc ctttgggaaa ctggtgccaa
     1441 aagggctggc cgatggtggg ggttgagggg ctggcggttt gccgcttcac tatcaggccc
     1501 gccaaataag cactgcggcc cgccgcaatg gcagcggcaa aggccttggc cattaggaca
     1561 gggtcggcag ctttagccac ggcagtgttg agtagcactg catcaaagcc catttccatg
     1621 gcctcacagg cctgggaggg tgcccctaag cccgcgtcca ccactaaggg cacattcggt
     1681 agccgctccc gcagggtttt taagttgtat ttattcatca ggccaaggcc tgtgcctatg
     1741 ggggagcccc agggcattaa cacctggcag ccaacgtcca acagcttttg gcacagcact
     1801 aaatcatcgg tgcaataggg cagcacctta aaaccccgcc gggtgagttc atccgccgcc
     1861 tccaccaagc caaacgggca gggctgcagg ttgtaccgcc cagaccgtgg cgcgcgaact
     1921 cggccgtc
//
LOCUS       DAOQGS010000728         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41435_length_1926_cov_2.998931, whole genome shotgun sequence.
ACCESSION   DAOQGS010000728 DAOQGS010000000
VERSION     DAOQGS010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41435_length_1926_cov_2.998931"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..698)
                     /locus_tag="PKZ52_18240"
     CDS             complement(138..698)
                     /locus_tag="PKZ52_18240"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNG61767.1"
                     /translation="MSHQYLYQSPRIGVRGLSAKELEGNYLHWFSDASVCAFNSHFAY
                     PKTQETVQQFILQLSGSLDHIVWAIYDRAEGVHVGNIGLRIFNRIDNNAELSFLLGEK
                     AYWGRGYGYEAGQMMIGHGFNVLNLQRIYCGCASTNIPMQKLAAKLGFVQEGVRRKNL
                     YLWGKYEDEIEYGLLRNEFVPADLNQ"
     gene            complement(977..1651)
                     /locus_tag="PKZ52_18245"
     CDS             complement(977..1651)
                     /locus_tag="PKZ52_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNG61768.1"
                     /translation="MNYHKHIHTGEGGICVTNDDYLAERMRLIRNHAEAVVDKKGYEP
                     IHNMIGFNFRLGEIEAALGRCQLKKLQEKVDQRFALGVALQKKMAGLKGLYTTPIAPG
                     NTHAFYVFGMRLDVRALGVSRDKIFAALIAEGVPALSKQYVNVHRYAMYRKKIAYGKE
                     HFPWRNGLYESSVDYTKGICPVAEQLQDQTYLGLHLCQYDFNLDDVEAIGQAFHKVWS
                     QLDAIK"
     gene            complement(1648..>1926)
                     /locus_tag="PKZ52_18250"
     CDS             complement(1648..>1926)
                     /locus_tag="PKZ52_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61769.1"
                     /translation="VALDGDPGPRERLADRAALWPARVLQGYSATVSRVCGPGFHMVG
                     NATEFLDPVFSSGVTLALESAHRGAATLLRELGGEEVDWDDAYGAVLV"
BASE COUNT          493 a          544 c          436 g          453 t
ORIGIN      
        1 gctggcaaaa aacccccacc gcaggcccac ttaataaaca gtcaaactgc acatctgccg
       61 gctgatgggc cgcctaggcc gccaataaag cagcgccgcc cgcgttatgg ctcactaata
      121 aataacgcat gggcaactta ttgattcaag tcggcgggta caaattcgtt gcgcaataat
      181 ccgtattcaa tttcatcctc atacttgccc cacaaatata agtttttacg ccgcaccccc
      241 tcttgcacaa aaccgagttt ggcggccaac ttttgcatgg gaatattcgt gctggcacag
      301 ccgcaataaa ttcgctgcag gtttaaaaca ttaaatccat ggccaatcat catctgcccg
      361 gcttcatacc cataaccgcg gccccaatag gctttttccc ccagcaaaaa acttaattca
      421 gcattattgt cgatacggtt aaatatccgc aggccaatat tccccacatg cacgccctcg
      481 gccctatcgt aaatggccca gacgatgtga tccagactgc cggataattg caggataaac
      541 tgctgcacag tctcctgggt tttagggtag gcaaaatggc tgttgaaggc acacacactg
      601 gcatcgctaa accaatgtaa atagttgccc tccaactctt tggccgataa tccgcgcacg
      661 ccaatacggg gggattggta gagatattga tgactcacag agcctcctat taatttttat
      721 cgctgtgatc aacctggtaa caatctgctt gatcgcactg atctattttg acttcgctgc
      781 actaaattta cgaaaatgcg tggaatttct tgttttagtg cagctcgcgg cggttcgcac
      841 cgccggggcg gcatccttgc cgccccaatt gaccctgcat aaaaagctaa ctcggcgcta
      901 attgccgccg ggttaatata tggttttatt tatttgggcg aagcgcgcaa aaaagataat
      961 ttaaaaagta cccacattat ttaatagcat ccagttgcga ccacacctta tgaaaggcct
     1021 gaccaattgc ttcaacatca tccaggttaa aatcatactg gcacaggtgc aggcctaaat
     1081 aggtttgatc ctgcaactgc tcagcgacag ggcaaatgcc cttggtgtaa tccacagaac
     1141 tttcgtacaa gccattgcgc caggggaaat gctctttgcc ataagcaatt tttttgcggt
     1201 acatagcgta acgatgtaca tttacatatt gtttagatag cgcaggcacg ccttcggcaa
     1261 ttaaggcagc aaaaatttta tctcggctca cacccagggc gcgcacatct aaacgcatcc
     1321 caaatacata aaatgcatgt gtattacctg gcgcaatcgg cgtggtatac aagcctttta
     1381 aacccgccat ttttttttgc aaggcaacac caagggcaaa acgctggtct actttttcct
     1441 gcaatttttt aagctgacaa cgccctagcg ctgcctcgat ttcccccagg cggaagttaa
     1501 aaccaatcat attatggata ggctcatagc cttttttatc caccaccgct tccgcatggt
     1561 tgcgaataag ccgcatacgc tcggctaaat aatcatcgtt ggtaacacag attccacctt
     1621 cgccagtgtg aatatgttta tggtaattca aactaaaacc gccccgtacg cgtcgtccca
     1681 gtcgacctcc tcgccgccaa gctcccggag cagcgtggcg gcgccgcggt gggccgactc
     1741 cagcgccagc gtcacgccgg acgagaacac cggatcgagg aactccgtcg cgttgccgac
     1801 catgtggaac ccgggcccgc acacgcggct gacggtggcg gagtagccct ggagcacgcg
     1861 cgccggccac agggccgccc ggtcagcgag ccgctcgcgg gggccggggt cgccgtcgag
     1921 cgccac
//
LOCUS       DAOQGS010000729         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41560_length_1922_cov_5.092662, whole genome shotgun sequence.
ACCESSION   DAOQGS010000729 DAOQGS010000000
VERSION     DAOQGS010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41560_length_1922_cov_5.092662"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKZ52_18255"
     CDS             complement(<1..758)
                     /locus_tag="PKZ52_18255"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNG61770.1"
                     /translation="MADLHSLAPGYTLTIALILGLLIGSFLNVVIYRLPLMMMREWKA
                     SVAEAEALVLPELPERFNLATPRSRCPHCQAPITALQNVPVVSFLLLKGRCSSCKTSI
                     SPRYPIVEAVTGLLSLVVVWHLGVGWVGLLGLVLVWTLVALTMIDVDHQLLPDNLTLP
                     LLWLGLVANSFSLFVPLSQAVWGAVVGYLSLWSVYWVFKLLTGKEGMGYGDFKLLAAL
                     GAWMGWQAVPLIIVLSSLVGAVIGGAAMILAGRGR"
     gene            complement(767..>1922)
                     /locus_tag="PKZ52_18260"
     CDS             complement(767..>1922)
                     /locus_tag="PKZ52_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNG61771.1"
                     /translation="KNVSGEISSGSVTSAKAQLIKQGIKATSVKKRPKPRGGGHAIKP
                     LDIAFFTRQMATMMKAGVPLVQSFDIVAEGVEKKSLADLIKSIRDEVASGTAFAKALE
                     KHPKYFDDLFCSLVESGEQSGALETMLDRIAIYKEKTERLKAKIKKALTYPIAVVVVA
                     IIVTGVLLVKVVPQFAETFGSFGAKLPAFTLFVLGLSNFAQQYWWVILIALVGCIVSI
                     SYLKRTNKRFAHLIDKLMLRIPIVGKIVFESIIARYARTLSTTFAAGVPLIDALESVA
                     GATGNIIYEQASLKVRDDVATGTQLNTSMRATRLFPSILLQMTAIGEESGALDEMLDK
                     VATYYEESVDNQVDSLTSLLEPMIMAILGVLVGGLLVAMYLPIFQLGNVV"
BASE COUNT          542 a          519 c          408 g          453 t
ORIGIN      
        1 cgcccgcgcc cagccaaaat catagccgcc ccaccaataa cggcgcctac caaggaggat
       61 aaaacaataa tcaacggcac cgcctgccag cccatccagg cccccaaggc cgccaacaat
      121 ttaaaatccc cataacccat gccctccttg ccggtcagca acttaaacac ccaataaacc
      181 gaccacaaac tcaaataccc caccaccgct ccccacactg cctggctgag ggggacaaac
      241 aggctaaaac tattggccac caagcccagc cacaaaagag gcagggtgag gttatccggc
      301 agtagttgat gatccacatc gatcatggtc aaagccacca gggtccacac caacaccaac
      361 cccaatagcc ccacccagcc cacacccaaa tgccaaacca ccaccaggct cagcagtcct
      421 gtaactgctt caacaatcgg gtaacggggg gaaatggacg ttttgcaact tgaacagcgc
      481 ccctttagga gcaggaaact cacaacggga acattctgca gtgccgtaat aggagcttgg
      541 cagtgcgggc agcgagagcg gggtgtggct aaattaaagc gctctggtaa ctccggcagc
      601 accaacgctt cggcctcagc aacagacgcc ttccattcac gcatcatcat gagggggagg
      661 cggtagataa ctacgtttag gaagctgccg attaacaatc ccagtattag ggcaatagtt
      721 aatgtgtagc ctggggctag ggagtgtaaa tcggccagcg tcacggttaa accacgttgc
      781 ccaactggaa gattgggagg tacattgcga ccagaagccc ccctactaat acacctagta
      841 ttgccatgat cattggttca agaagagatg ttagactgtc cacctgattg tcgaccgatt
      901 cttcataata ggttgcgacc ttatccagca tttcatctaa agcgccagat tcttcaccga
      961 ttgcagtcat ttgcagcaaa atcgacggaa ataagcgagt tgctctcatg gaagtattta
     1021 actgtgtccc agtcgcgacg tcatctcgaa cttttaaaga agcctgttcg tagataatat
     1081 taccagtcgc tccggccacc gattccaatg cgtcaatgag cggaacacct gcagcaaatg
     1141 ttgttgaaag tgttctggca taacgagcga ttatagattc aaagactatt ttccctacga
     1201 ttggaattct caacatcagc ttgtctataa gatgggcaaa tcgtttattt gtcctcttca
     1261 aataggaaat ggagactatg cacccaacta gtgctatcaa aatcacccac caatactgct
     1321 gtgcaaaatt tgaaagacca aggacaaata aagtaaaagc tggtagcttg gctccaaaac
     1381 tcccaaatgt ttcagcaaac tgcggcacaa cttttaccaa taacacccca gtaacaatta
     1441 tcgcaactac aacaactgct atgggatatg tgagcgcttt tttgattttg gctttaagcc
     1501 tctcggtttt ttctttatag attgcaattc gatccaacat tgtttcaagc gcgccagatt
     1561 gctcgccaga ctctacaagg ctacagaata aatcatcaaa atattttggg tgtttctcta
     1621 gtgccttggc aaatgcagta cctgaggcaa cttcatccct aatagacttt atcaagtcag
     1681 caagcgactt tttttctact ccctccgcga caatgtcaaa gctttgaacc agcggaaccc
     1741 cggctttcat catggtagcc atttggcgag taaaaaatgc tatgtccagg ggcttaatag
     1801 catgcccgcc tcctctgggt ttggggcgct ttttaactga tgtcgcttta attccttgtt
     1861 ttattagctg cgctttggcg ctggtgacac tgccgctgga tatttcaccc gagacattct
     1921 tg
//
LOCUS       DAOQGS010000730         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41807_length_1916_cov_3.557765, whole genome shotgun sequence.
ACCESSION   DAOQGS010000730 DAOQGS010000000
VERSION     DAOQGS010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41807_length_1916_cov_3.557765"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKZ52_18265"
     CDS             complement(<1..557)
                     /locus_tag="PKZ52_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNG61772.1"
                     /translation="MTDLRLADLPPQTQALRGRVILITGASSGIGRAAALHCAEQGAT
                     VILVGRDVKKLEAVYDAIEAANFPQPAIFELNLKTANEQHYTALAKVIDEEFGQLDGL
                     LHNAGELGQLCPLEQISPAVWHEVVQVNLTGPFLLTRALLPLLRKGSAARILFTSSSV
                     GRKGRAFWGVYAVSKAGIENLTQTLA"
     gene            complement(589..1248)
                     /locus_tag="PKZ52_18270"
     CDS             complement(589..1248)
                     /locus_tag="PKZ52_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNG61773.1"
                     /translation="MPLRALLFDLDGTLLDTSGDMGQALNRLLNEEGLPSLPAETIRP
                     QVSNGANALVKLGFGELPGAAHEQLRGRFLDLYLADIARHTRPFPGIEALLQRCLQQG
                     IAWGVATNKPWRYTEPLMRHFSFASSAAVTLCPEHAKPKPDPAMLLLACEQMGCAPGE
                     VIYIGDHQRDIECGKRAGMATIAVGYGFTQSPDEHTHWDATFAVQQAAEIWPLVQQHY
                     L"
     gene            complement(1256..>1916)
                     /gene="ubiG"
                     /locus_tag="PKZ52_18275"
     CDS             complement(1256..>1916)
                     /gene="ubiG"
                     /locus_tag="PKZ52_18275"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNG61774.1"
                     /translation="LASRWWDPAGECKPLHLMNPVRANYIDGQSAVAEKRLLDIGCGG
                     GLLAEAMAQRGAVVTAIDLSEDALQVARLHALESQVTIDYQLTSAEAFAEQHPGEFDL
                     VTCLEMLEHVPEPASVVQAAANLCAPGGAVYFSTLNRTAQAYGLAVVGAEYLLKLLPK
                     GTHDYQRFIRPSELAAMCRQAGLIVRDITGLSYNPINQSFRLSGRDVDVNYLVYCQKP
                     L"
BASE COUNT          355 a          541 c          625 g          395 t
ORIGIN      
        1 gcgagggttt gggtgaggtt ttcaatgccc gctttactca ccgcatacac cccccaaaag
       61 gcccggccct tgcggccgac gctggagctg gtaaacaaga tgcgggccgc actgcccttg
      121 cgcagcagcg gcagtagggc gcgggttaat aaaaaagggc cggtgaggtt tacctgcacc
      181 acctcgtgcc aaacggcagg gctaatctgc tccagcgggc acagctggcc cagctcacct
      241 gcgttgtgca gcaggccgtc cagctggcca aattcttcgt cgattacctt agccagggct
      301 gtgtaatgct gctcgttggc agtttttaaa ttcagttcaa aaatcgcggg ttgggggaag
      361 ttggccgctt cgatggcgtc gtagacggct tcaagttttt tcacatcccg ccccaccaaa
      421 ataaccgtgg cgccctgttc tgcgcagtgc agggcggcgg cgcggccgat accgctgctg
      481 gcgccggtga ttaaaatcac ccgcccgcgc agggcttggg tttgtggggg gaggtcggca
      541 aggcgtaggt cggtcatggt ttcagcggta attaagtggg taaaaggttt ataggtagtg
      601 ctgctgcact aggggccaaa tttcggcggc ttgttgcacg gcgaaggtgg cgtcccagtg
      661 ggtgtgttca tccgggcttt gggtgaagcc atagcccacg gcgatggtgg ccatgcctgc
      721 tcgtttgccg cattcaatgt cccgttgatg atcgccaatg taaattacct caccgggggc
      781 gcagcccatc tgctcgcagg ccaacagcag catggccggg tcgggcttgg gtttggcgtg
      841 ctccgggcag agggttacag cggcgctgct ggcaaagcta aagtggcgca tcaatggctc
      901 ggtgtagcgc cagggtttat tggtggcaac gccccaggcg atgccctgtt gcaggcagcg
      961 ctgaagcaat gcttctatgc cggggaaagg ccgggtgtgg cgggctatat ccgccaggta
     1021 caaatccaaa aaccgcccgc gcaattgctc gtgggccgcc cccggaagct cgccaaaacc
     1081 cagtttcacc agggcgttgg cgccgttgga gacttggggg cgaatggtct cggcgggcag
     1141 gctgggcaat ccttcttcat ttaataagcg gtttaaggct tggcccatgt cgccactggt
     1201 gtccagcagg gtgccgtcta aatcaaataa caatgcgcgc aggggcatgg cggtctcaca
     1261 ggggtttttg gcagtacacc aggtaattta catccacatc ccggccggat aagcgaaagg
     1321 actggttaat agggttgtag cttaagccgg taatgtcccg cacaatcagc ccggcctggc
     1381 ggcacatggc ggccagttcg ctggggcgga taaaccgctg gtaatcgtgg gtgcccttgg
     1441 gcaacagttt tagcagatat tccgccccca ccacggccag gccataggcc tgggcggtgc
     1501 ggttgagggt ggagaaatac acggcgccgc cgggtgcgca caggttggca gccgcctgta
     1561 caacactggc aggctccggc acatgttcga gcatctcaag gcaggtgacc aggtcgaact
     1621 cgccagggtg ttgctcggcg aaggcttctg cgctggtgag ctgataatca atcgttacct
     1681 gagattccaa ggcgtgtagc cgcgccacct gcagggcgtc ttcgctgagg tcgatggccg
     1741 tcaccaccgc gccgcgctgg gccatggctt ctgccagcag gccgccgccg cagcctatat
     1801 ccagcaggcg tttttccgcc acggcacttt ggccgtcgat gtaattggcc cgcaccgggt
     1861 tcatcaggtg caggggtttg cactcccctg ctgggtccca ccagcggctg gcgagg
//
LOCUS       DAOQGS010000731         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41869_length_1914_cov_4.907477, whole genome shotgun sequence.
ACCESSION   DAOQGS010000731 DAOQGS010000000
VERSION     DAOQGS010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41869_length_1914_cov_4.907477"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..821
                     /locus_tag="PKZ52_18280"
     CDS             93..821
                     /locus_tag="PKZ52_18280"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNG61775.1"
                     /translation="MRLSQIIIGLSIFVAQFCLAETLFTATYKGKYNGWNITLERSLQ
                     EVTPGQFNLASKASNFLGQLEEVSEFTLADDQLVPRVYTYNRRILGKTNSESIRFAWN
                     SQEAFYTRANKSQDNRTLKLTPGLLDPALYQLQLQRDLARQQKELNYAFIKRKDIKSY
                     RFSPVGSDTFTLNGVQLKATLVERLENSAKKTRIWLLPELNWQIARIQHKEESGDTYT
                     IELARYSGDSALMNRLYQKPPPRK"
     gene            935..>1914
                     /gene="msrP"
                     /locus_tag="PKZ52_18285"
     CDS             935..>1914
                     /gene="msrP"
                     /locus_tag="PKZ52_18285"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNG61776.1"
                     /translation="MSCPKSPLIIVPPNNPIRPSEITSESAYLNRRELMASFIGGALA
                     GGAMGVQAAPSQIADENPPGPAWLKDKIAIAKPGVAIPEALTPYKHVTQYNNFYEFGT
                     AKTDPAKYAQTLKPDPWRVSISGAVEKPGQYFLDDLLKGIDFEERIYRLRCVEAWSMV
                     IPWVGFSLSELLKKAQPTGSAKYVAFTTLQDKAQMPEQNNRLSTIHWPYQEGLRLDEA
                     MHPLCILAVGIYGKVLPNQNGAPLRLVVPWKYGFKSIKSIVGITLTEKQPPTSWNQLA
                     PQEYGFYANVNPQVDHPRWSQAKERRLPNSLLNPNIVATQMFNGYAEEVA"
BASE COUNT          529 a          516 c          432 g          437 t
ORIGIN      
        1 cgacacagcg caagccattt ataaggccca caactaatct tgatcggtta actcaaaggg
       61 ggcctagccc ccttttttat gctgtgaatt ttatgcgttt aagccaaatt attatcggcc
      121 tgagtatttt tgttgcgcaa ttttgccttg cggaaacttt gttcacggcc acctacaaag
      181 gtaaatataa cggctggaat attacccttg aacgcagctt gcaagaggtc acgcccggcc
      241 aatttaatct tgcctccaag gccagtaatt ttttgggtca gttagaggaa gtaagtgaat
      301 ttacgctcgc cgatgatcag cttgtacctc gtgtatatac ctataatcgc cgcattttag
      361 gcaaaaccaa tagcgaaagt attcgctttg cctggaattc ccaggaggcc ttttatacgc
      421 gcgccaataa aagccaggat aatcgcacgc tgaaattaac ccctggtctt ttggatcctg
      481 cactgtatca actgcagcta cagcgggatc tcgcccgcca acaaaaggag ttaaattacg
      541 ctttcattaa gcgcaaagac attaaatcct accggttcag ccccgtggga agcgatacct
      601 ttaccctcaa tggtgtgcag ctaaaagcca ccctagtaga gcgcctagag aattccgcca
      661 aaaaaacccg catttggctt ttaccggagc ttaattggca aatcgcccgc atccaacata
      721 aggaggagtc tggcgatacc tataccatcg agcttgcgcg ctacagcggc gacagcgcac
      781 tgatgaaccg actctaccaa aaaccgccac cgcgaaaata acccttactt gaacccataa
      841 atcccctaaa ctgcctagaa cggggaacaa gctggttgaa gacatgtccc gttaagacgc
      901 ttctgtcatc ctaaacgtgt ccaggcaggt ttttatgtcg tgccccaaat ctccattgat
      961 tattgtccct cctaacaacc ccattcgacc tagcgaaatt acctctgaat ccgcctactt
     1021 aaatcgacgc gaactgatgg cgagctttat aggcggcgct ctggcgggcg gtgccatggg
     1081 ggttcaggca gcgcctagcc agattgccga tgaaaatccg cccggccctg cttggctaaa
     1141 agacaagata gctatagcca aaccaggagt cgcgatacct gaggcgctaa caccctacaa
     1201 acacgttacc caatacaata atttttacga attcggcaca gccaaaacag atcctgcgaa
     1261 atacgcccag acgttaaaac ctgatccctg gcgagtcagc atcagcggcg cggtggagaa
     1321 acccgggcag tattttcttg atgatctgtt aaaaggaata gattttgagg agcgcattta
     1381 ccgactgcgc tgcgttgagg cctggtctat ggtgatcccc tgggtgggat ttagcctaag
     1441 tgaactgctt aaaaaggcgc aacccactgg ctcagcaaaa tacgtcgcct tcacaacgct
     1501 ccaggacaag gcgcaaatgc cggaacaaaa taaccgccta tcgactattc attggcccta
     1561 ccaagaaggg ttgcgcctgg atgaagccat gcaccccttg tgtattttag ccgtcggaat
     1621 ctatggcaag gtgctgccca atcaaaacgg cgcgcctctg cgtttggtag tgccgtggaa
     1681 atatggcttt aaaagtatta aatccatagt gggcattaca ctaacagaaa agcagccacc
     1741 caccagctgg aatcaactgg ccccacagga atacggcttc tacgccaacg tcaacccgca
     1801 ggtcgatcac ccccgttgga gtcaagctaa agagcggcgc ctccccaata gcctgttaaa
     1861 ccccaatata gttgccacgc aaatgttcaa tggctatgcc gaagaagtag caga
//
LOCUS       DAOQGS010000732         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41872_length_1914_cov_4.086606, whole genome shotgun sequence.
ACCESSION   DAOQGS010000732 DAOQGS010000000
VERSION     DAOQGS010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41872_length_1914_cov_4.086606"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..969)
                     /locus_tag="PKZ52_18290"
     CDS             complement(166..969)
                     /locus_tag="PKZ52_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HNG61777.1"
                     /translation="MNPSNCPNPAQLNRRETLGMLSLLGAVSLAGCAGSKSGASGSAA
                     SSSSSSSSSSNSSSSTSSSSSGGVGQCVLIPQETIGPYPLSAILSNSAMVRRDVTEGK
                     SGVPLRLTFKLVNTQQGCNSFPYASVYIWHCDKDGVYSGYSQPGANTVGQTFLRGIQD
                     TDANGEVTFNTIFPGWYNGRVTHIHFQIFLYNNLSGNGVAISQLAFPDNVVRAVYNSP
                     LYSARGQNSLSLSADNVFSDGTQYQMASITGDINNGFEAFLQVGIAAGA"
     gene            complement(974..1513)
                     /locus_tag="PKZ52_18295"
     CDS             complement(974..1513)
                     /locus_tag="PKZ52_18295"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNG61778.1"
                     /translation="MKRFPLLLLSLSALICLPAAAEAPAPGLWQLSFTHAGAPAERPE
                     PRASERPNSAGDEGRAPRGEGPGAGNPPVHTLCLTASDDPNQVFFMPPRHRQRADGPP
                     PCTLTYLSQTDASANWSLSCDGPRGVMQAQGQAQFSADHYSASLSFASETRGPEHGRM
                     VTASRLGDCSAAPAITDQE"
     gene            complement(1626..>1914)
                     /locus_tag="PKZ52_18300"
     CDS             complement(1626..>1914)
                     /locus_tag="PKZ52_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61779.1"
                     /translation="ITDVPPFCLHGLNAVYLPKWGWYRLDARGNKAGVNAAFSPPLEQ
                     LAFNLANPGEEDLPGLFAEPLPAIVQVLSSSANIAEVFKRLADTDNQAQLR"
BASE COUNT          411 a          520 c          591 g          392 t
ORIGIN      
        1 ctacacggat gtattcacgg cgtgtctgaa atgatgtgta ttcattgctg gcgcctaggt
       61 agtagctagc cttgcccaca ccaaaagcca cagcattttg gatatgtttt ataaaaaagg
      121 ccgagtgatc tcggccaaag cacggcaaca cctatttaaa aaacgctaag ccccggcggc
      181 aatccccacc tgcaaaaacg cctcaaagcc gttatttata tcccccgtga tactcgccat
      241 ttgatattga gtgccatcgc taaaaacatt atcggcgctg aggctgaggc tgttttgccc
      301 ccgtgcactg tacaaaggcg agttgtacac cgcgcgcaca acattatcgg gaaatgccag
      361 ttgcgatata gccaccccat tgccgcttag gttgttgtac aaaaatattt gaaaatgaat
      421 atgggtgacg cggccgttgt accaaccggg gaaaatagta ttaaaagtca cttcaccatt
      481 ggcatctgta tcttgaatgc cgcgcaaaaa tgtctgcccc accgtgttgg caccgggttg
      541 ggaataaccg gaatacacac cgtctttatc gcagtgccaa atgtatacag acgcataggg
      601 aaagctgttg cagccttgtt gtgtgttcac caatttaaat gtcagccgca ggggcacacc
      661 gctttttcct tcggtgacat cccgacgcac catggcggaa ttactcaaaa tagccgagag
      721 cggatagggg ccgatggttt cttggggaat taatacacat tgcccaacgc cgccggaaga
      781 actgctggat gtgctggaac tggaattaga gctggaactc gagctagaac ttgagctagc
      841 cgcagagcca gaagcgccgg atttactgcc tgcgcaaccg gcgagactca ccgccccgag
      901 caggctgagc ataccgaggg tttcccggcg gttgagctgg gcgggattgg ggcaattgga
      961 tggattcata aacctactcc tgatcagtaa tggcaggagc agcggagcaa tcgcccaaac
     1021 ggctggcggt gaccatgcgg ccgtgctcag gccctcgggt ttcgctggca aaacttaggc
     1081 ttgcgctgta gtggtcggcg ctgaactgcg cctgcccctg agcctgcatc accccgcgcg
     1141 ggccgtcgca gctgaggctc cagttggcgc tggcatcggt ttgcgacaaa taagtaagcg
     1201 tgcagggcgg cgggccatcg gcgcgctggc ggtggcgcgg cggcataaaa aacacctggt
     1261 tgggatcgtc gctggcggtt aaacacaggg tgtgtacggg cgggttgccc gcaccggggc
     1321 cctcaccccg aggggcgcgg ccttcatccc cggcgctgtt gggccgctcc gaggcgcgag
     1381 gctcagggcg ctcggcgggc gcgcctgcgt gggtaaaact taactgccag agcccggggg
     1441 ccggtgcttc ggcggcggcg ggcaggcaga tcagggcact gaggctgagt aataggagtg
     1501 gaaatcgctt catgggatgg gtcctgtgca agtggcaaaa ccccgtcagg ggagttgcct
     1561 ttagcctgca gcaggactgt atcggctata ccgccagtgt gtaaccactg ggtatggggt
     1621 ttgtatcagc gcagctgggc ttggttgtcg gtgtcggcca ggcgcttaaa cacctcagca
     1681 atgttggcgc tgctgctgag cacctgcact atggcgggca gcggctcggc gaacaggccg
     1741 ggcaggtcct cctcgcctgg gttggcgagg ttaaaagcca gctgctccaa gggcgggctg
     1801 aaggcggcgt tcacgcccgc tttgttgccc cgcgcatcca gccgatacca gccccatttg
     1861 ggcaggtaca cggcgtttag gccgtgcagg caaaacggcg gcacatcggt gatg
//
LOCUS       DAOQGS010000733         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_41992_length_1911_cov_2.810884, whole genome shotgun sequence.
ACCESSION   DAOQGS010000733 DAOQGS010000000
VERSION     DAOQGS010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41992_length_1911_cov_2.810884"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKZ52_18305"
     CDS             complement(<1..257)
                     /locus_tag="PKZ52_18305"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HNG61780.1"
                     /translation="MRNLWLLLAMVLCAGAAIAITPRHKIADRNPMPNLSTIIPQQFG
                     QWKQIPGSNQIIDPGQAQKLNSIYDQVYARTFENKRGYRVM"
     gene            complement(269..1153)
                     /gene="xrtB"
                     /locus_tag="PKZ52_18310"
     CDS             complement(269..1153)
                     /gene="xrtB"
                     /locus_tag="PKZ52_18310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase B"
                     /protein_id="HNG61781.1"
                     /translation="MTDAQPLTQAPSTSGKAQLIEWAPVLLGLAVLFIPTYWRLGSTI
                     WVTDEQGHGPIILGLALWYIAQQRNRIHTLTAKPQTGLGLAFFMVGLLAYCLGRSQDI
                     LLLEVGAQIPVLMGLLLIARGWAAVKILWFPLFFLIFMVPLPGGLVDALTMPMKIAVS
                     WGAEHILWYFDFPVSRSGVILAVGQYKLLVADACAGLHTLFTLEALGLLYLHWVKHES
                     YFRNITLAILIVPISYTANTIRVIILCLVTYYYGDEAGQGFIHGFAGMVLFLTALIFI
                     IGVDSTLQYYAKRFGANK"
     gene            complement(1168..>1911)
                     /locus_tag="PKZ52_18315"
     CDS             complement(1168..>1911)
                     /locus_tag="PKZ52_18315"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNG61782.1"
                     /translation="GQRFGEAAVSLGLVEESQLAQSLARQYGFTYLAPNDSRIHASVV
                     TAFTPDNLAVEQYKNLRSQLALRWFNQGNQLLAICSLEAGDGRSLTAANLAVVYAQMG
                     KKVLLIDADLRTPSQHLLFSEANTTQGLSSVLAGLHSFESAISPIAAMPGLSLLAAGA
                     HAPNPTELLASNRFSHALDVLTKHYDIILIDTPAGNLYPDSLGLARLAQGALLVARKN
                     TTGLTPLKQYADQLTQIGVSLVGSFLTFG"
BASE COUNT          452 a          458 c          561 g          440 t
ORIGIN      
        1 aacattactc tatagccacg tttattttca aaagttcttg catatacctg atcgtagatt
       61 gaatttaatt tttgcgcctg cccagggtct attatttggt tgcttcctgg aatctgtttc
      121 cattgaccaa attgttgtgg gatgattgtt gataggtttg gcatcggatt gcggtcagcg
      181 attttatgcc ttggtgtaat tgcaattgcc gcgcctgcgc acaataccat tgctagtagc
      241 aaccataggt tacgcatgga gactccattc atttattagc tccaaagcgt ttggcatagt
      301 attgaagcgt gctatccacg ccgataataa aaattaacgc cgtgaggaat aacaccatgc
      361 cggcaaagcc gtgaataaac ccctgccctg cttcatcgcc ataataataa gtaaccaaac
      421 acagaataat tacgcggatg gtattggcgg tgtaggagat gggcactatt aaaatcgcga
      481 gtgtgatatt gcggaagtag gactcgtgtt ttacccagtg cagatataac aggcctaagg
      541 cttctaaggt aaatagggtg tgcaggcctg cgcaggcgtc ggctactaat aatttgtatt
      601 ggcccacggc caaaatgacg ccgctgcggg aaacggggaa atcaaaatac cagaggatat
      661 gttctgcgcc ccaggagacg gcgattttca tcggcatggt gagggcgtct actagcccgc
      721 cgggcagtgg caccataaaa attaaaaaaa acagtggaaa ccaaagaatt ttgaccgctg
      781 cccagccgcg tgcaatgagt aataaaccca tgagcacggg aatttgcgcg cccacttcca
      841 gcaataaaat atcctgcgag cggcccaaac aataggccaa taggcccacc ataaaaaacg
      901 ccaggcccaa accggtttgg ggtttggcgg tgagcgtgtg aatgcggttg cgctgttggg
      961 caatgtacca gagggcaagc cctaggatga tggggccgtg gccctgctcg tcggtaaccc
     1021 agatggtgct gcctaagcgc cagtaggtgg ggataaacag cacggcaagg cccagcagta
     1081 ccggcgccca ttctattaac tgggctttgc cgctagtgct tggggcctgg gtgagtggct
     1141 gtgcatcagt catataaagg ccgcaggcta gccaaaggtt aaaaacgaac ccaccaggct
     1201 tacgccaatt tgcgtgagtt ggtcggcata ttgtttcagc ggggtaaggc cagtggtgtt
     1261 tttgcgcgca accaataaag cgccctgggc cagccgggca aggcctaggc tatccgggta
     1321 gaggttgccc gccggggtat cgatcaggat gatgtcgtaa tgtttggtga gcacatccag
     1381 cgcgtgggaa aagcggttgc ttgccaatag ttcagtgggg ttgggggcgt gggcgcctgc
     1441 cgccagcagg cttaaacccg gcatagccgc tatggggctg atggcggatt caaaactgtg
     1501 caagcccgcg agcacagaac ttaggccctg ggtggtattg gcttcgctaa agagtaaatg
     1561 ctggctgggt gtgcgcaggt ccgcatcaat gagcaggact tttttgccca tttgtgcata
     1621 caccacagcg aggttggcgg cggtgaggct gcggccatcg cctgcttcca ggctgcaaat
     1681 ggccagcagc tggttgccct ggttaaacca gcgcagggcc agttggctgc gcaggttttt
     1741 atattgctcc acggccaggt tatccggcgt aaaggccgtc actacgcttg cgtgaatgcg
     1801 gctgtcgttg ggggctaggt aggtaaaacc gtattggcgc gcgagggact gggcgagctg
     1861 gctctcttcc actaggccta ggcttacggc ggcttcgcca aagcgctggc c
//
LOCUS       DAOQGS010000734         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_42166_length_1906_cov_3.202053, whole genome shotgun sequence.
ACCESSION   DAOQGS010000734 DAOQGS010000000
VERSION     DAOQGS010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42166_length_1906_cov_3.202053"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1906)
                     /locus_tag="PKZ52_18320"
     CDS             complement(<1..>1906)
                     /locus_tag="PKZ52_18320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNG61783.1"
                     /translation="MEYIEGQDLKQARKQLNLQQKIQAVQDIARALDFAASKGYVHRD
                     IKPENIMIAKADHRAVLMDFGIARAAETDLSVTQTGLAIGTPHYMSPEQAKGKAVDAR
                     SDLYSLGVVFYLLLTGKVPFDGDSAVAIGIKHITEPPPVLPESLAVAAPILNRLLAKL
                     PGDRYQQAEDLVAALEELKPLLLDAKPLPEEDSPPPAWASAATLVEVSPGIAPQSMDS
                     LAALEATGVLASQPTGILSSQAAGQHKAAEVASGGLTWFGVGLILSLAAAAAALGIWY
                     VNKPEPAASFAALAAGNNKDVKQLKENIALVTKAYQKSESQLPVLVKLYRDLKKLQPD
                     DPSVEQELTFLAQQQLKRLTWALNLADYEQAVLLNKNLKLLFPEFVTEELNRQEMRLS
                     LRAQIQALLLNGEDYVKRGALAQPPGANAREAYEAVLALDPDIAPAREALANIRQAPK
                     DSSRITTAQIAAAPLNTADKLALAKRLAEKGQLFAPAGQNAYELYAQILLKDPGNPAV
                     REASQSLWQSLPGWANRWVKEGAYNTVNEQLTAAGEIKPNDKALQQLHQSIDEKIVER
                     NRNILPQVTGLRVAGFPQDSFDEPQASSLSVSKHLHVRLALAQSSPQILQVNLYEGAR
                     TPHRSCGIALA"
BASE COUNT          378 a          539 c          541 g          448 t
ORIGIN      
        1 ggcgagggcg atcccgcagg agcggtgcgg ggtgcgggcg ccctcgtaga gattaacctg
       61 caatatctgt ggcgacgact gcgccagagc taggcgcaca tgcagatgtt tactcaccga
      121 taggctgctc gcctggggtt catcaaaact gtcctgggga aaaccggcaa cgcgtaaacc
      181 cgtcacctgg ggcagtatat tgcggttgcg ctcaacaatt ttttcatcga tggattgatg
      241 caactgctgc agcgccttgt cgttgggttt gatttcaccc gcggcggtta gctgctcatt
      301 caccgtgttg taggccccct ctttgaccca gcgattggcc cagccgggca ggctctgcca
      361 gaggctttgg ctggcttcgc gcacagcggg gttgccgggg tctttaagta aaatctgcgc
      421 gtacagctca taagcatttt gcccggctgg cgcaaataac tgccccttct cggctaaacg
      481 cttggccaag gctaacttgt ctgcggtgtt taaaggcgcg gcggcgattt gagccgttgt
      541 aatgcggctt gaatcctttg gcgcctggcg gatattggcc agggcctccc gagccggggc
      601 tatgtctggg tctagagcca acaccgcttc ataggcttcc cgtgcattgg cgccgggcgg
      661 ctgggcaagc gcaccgcgct tgacgtaatc ctcgccgttg agcagcagcg cttgaatctg
      721 tgccctaagg ctcagacgca tttcctggcg atttaattct tcggtaacaa attcaggaaa
      781 taacagcttc aagtttttat ttaacaacac cgcttgctca tagtcggcaa gatttaacgc
      841 ccaggtcagg cgcttgagtt gttgctgggc taaaaaggtt aactcctgct cgacgctcgg
      901 atcatcaggc tgcagctttt ttagatcccg gtagagcttc accagcaccg gcaattggct
      961 ttcacttttt tgataggcct tagtgaccag ggcgatattt tcttttaatt gtttaacatc
     1021 tttattgtta cctgccgcca aggctgcaaa ggatgcggca ggctcaggct tattcacata
     1081 ccaaatgccc agggctgcgg cagccgcggc caggcttaaa atcaggccaa ccccaaacca
     1141 cgtaagccct ccagatgcca cctcggcggc cttatgttgc ccggccgcct ggcttgaaag
     1201 tatcccggtc ggttgtgatg cgaggacacc cgtagcctcc agggccgcca atgagtccat
     1261 tgactggggc gcaatcccag ggcttacttc caccagggtt gccgcactgg cccaggcggg
     1321 cggcggactg tcttcttccg gcaggggttt ggcgtcaagc agtagcggtt ttaattcctc
     1381 aagggctgcc accaaatcct ccgcctgctg gtagcgatcc ccaggcaatt tggccagcag
     1441 ccgattgagg atgggcgccg ccacggctaa ggattccggt aaaaccggcg gcggctcagt
     1501 aatgtgttta atacctatgg ccaccgcaga atcgccatca aagggcacct tgccggtgag
     1561 caataaataa aacaccacac ccaggctgta gaggtcagat cgcgcatcca cggccttgcc
     1621 cttggcctgc tccgggctca tgtaatgggg cgtgcctatg gcaaggccgg tttgggtcac
     1681 gctcaggtcg gtctcagcgg cgcgggcgat gccaaaatcc atcagcacag cgcgatgatc
     1741 agccttggcg atcatgatgt tttccggttt gatatcccgg tgcacataac ctttgctggc
     1801 ggcaaaatcc aaggcgcggg cgatatcctg cacagcttgg attttctgct gcaaattgag
     1861 ctgcttgcgg gcctgtttta aatcctgccc ctcgatgtat tccata
//
LOCUS       DAOQGS010000735         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_42483_length_1898_cov_3.489962, whole genome shotgun sequence.
ACCESSION   DAOQGS010000735 DAOQGS010000000
VERSION     DAOQGS010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42483_length_1898_cov_3.489962"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PKZ52_18325"
     CDS             complement(<1..1180)
                     /locus_tag="PKZ52_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNG61784.1"
                     /translation="MPPVYLRLAIPSPLNRLFDYLPPADLSPAQAAGLQPGLRCQVSF
                     GGQKAVVGFLIEVAQHTEVPEEKLKAVDAILDSTPLLSAANLALAQWVASYYQHPLGE
                     VLWGALPGVLRKGGPLPQAKAWRHSFEGKGLGPTAIKRNKSQQAIHQYLLDEGVLTQE
                     QCRALGFKAATLKQLIHKGLVEETSLSTSIKAPRAILREPHQRANEEQQAALDSISLG
                     QFQAVLLDGTTGSGKTEVYLQLIGRALEAGKQALLLVPEIGLAPQTLARFRQRFCVPL
                     VELHSNLSEGERVKHWWAAAQGEARIVIGTRLALFTPLADLGVIIVDEEHDASFKQNE
                     GLRYSARDLSLVRGQLEQVPVVLGSATPSLESIHNAQAGRFSHLRLSQRAGAAQPPQL
                     S"
     gene            1318..1530
                     /gene="rpmE"
                     /locus_tag="PKZ52_18330"
     CDS             1318..1530
                     /gene="rpmE"
                     /locus_tag="PKZ52_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNG61785.1"
                     /translation="MQESIQPKYGPLVATCSCGNVINTRSTLSKPIHIDVCSECHPFY
                     TGKQKTVDTGGRIDRFKKRFAGIRAS"
     gene            complement(1668..>1898)
                     /locus_tag="PKZ52_18335"
     CDS             complement(1668..>1898)
                     /locus_tag="PKZ52_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61786.1"
                     /translation="IWMSFMVQALEGQPEVIHAQPAGLVTIKINPDTGNRAEPGEPDA
                     VFEYFKAGEGPEENPASDSSDNPQQKLPDDLF"
BASE COUNT          400 a          531 c          530 g          437 t
ORIGIN      
        1 ggctgagctg cggcggctga gcggcgcccg ctctctggct caagcgcaaa tggctaaagc
       61 ggcccgcttg ggcattgtgg atggactcca aagacggggt ggcgctgccg agcaccaccg
      121 gcacctgctc caattggccc cgcaccaggc ttaggtcccg ggcggaatag cgcaggccct
      181 cattctgttt aaaggaggca tcgtgctctt catccacgat gatcacaccc agatccgcca
      241 ggggcgtaaa tagggcgagg cgggtgccga tcactatgcg cgcctcgccc tgtgctgccg
      301 cccaccagtg tttaacccgc tccccttcgc ttaggttgga gtgcagttcc accaagggca
      361 cacaaaagcg ctggcgaaag cgcgccaggg tttggggcgc aaggccaatt tccggcacca
      421 ggagcaaagc ctgtttgccc gcttccaacg cccggccaat cagctgtaag tacacctccg
      481 ttttgccgct gccggtggtg ccatcgagca gcaccgcctg aaactggccg aggctgattg
      541 aatctagggc tgcctgttgc tcttcattgg ctctttgatg aggttcgcgc aggatggcgc
      601 ggggtgcctt gatactggtg cttaaggatg tttcttcaac caggcccttg tggattaatt
      661 gtttgagggt ggcagcttta aagccaaggg cgcggcactg ctcctgggtg agaacgcctt
      721 catctaacag atattgatga atggcttgtt ggctcttatt gcgcttaatg gcggttggtc
      781 ctaagccctt gccctcgaaa ctgtgccgcc aggctttggc ttggggcagg gggccacctt
      841 tgcgcagtac accgggcagt gccccccaga gtacctcgcc taagggatgc tgataatagc
      901 tagccaccca ttgagccaag gcgagattgg cggcgctcaa tagcggcgtg gagtctaata
      961 ttgcatccac cgccttgagt ttttcctcag gtacctcggt gtgttgggct acttcaatta
     1021 aaaaccctac taccgccttt tgaccgccaa aggacacctg gcaccgcaac cctggttgca
     1081 aaccggctgc ctgggcgggg cttaagtcgg cgggggggag gtagtcaaac aggcgattta
     1141 agggggaggg gatggctaag cgcaggtaaa caggcggcat ggagtctatt tgattcggtt
     1201 aatgcttgcg cagcgtaagc tttattaata gtatgcgcgc tcttttttaa ccacccacaa
     1261 taacggctgt gcggtgctcg gcacaagttc gagtggcggc acacatcgag gtttattatg
     1321 caagaatcta ttcaacccaa atacggccca ttagttgcca cctgcagctg tggcaacgtg
     1381 atcaacacgc gctccacgct ttctaagcca atccacatcg acgtgtgttc tgagtgccac
     1441 cctttttaca ctggcaagca aaaaaccgtt gatactggcg gccgtatcga ccgcttcaaa
     1501 aagcgttttg ctggtatccg cgcttcctaa gcaaagtttg caagcaataa aaaaggcgcc
     1561 tggtgcgcct tttttattgc ctagagtttt ttgctgaggc tcaagcgaag agcctcaacc
     1621 gagttattaa cgaatgtggc aagcacctaa agtgcttccc aaccggccta aaacaaatcg
     1681 tccggcagct tctgctgcgg attgtcggag gaatcgctgg ccggattctc ctccgggcct
     1741 tcacccgcct tgaaatattc aaatacagca tcgggctcgc caggctcagc gcgattgccc
     1801 gtatccgggt taattttaat cgtcactaag cccgctggtt gtgcgtgaat aacctccggt
     1861 tgtccttcta gggcctgcac cataaacgac atccaaat
//
LOCUS       DAOQGS010000736         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_42523_length_1897_cov_3.620521, whole genome shotgun sequence.
ACCESSION   DAOQGS010000736 DAOQGS010000000
VERSION     DAOQGS010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42523_length_1897_cov_3.620521"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1747
                     /locus_tag="PKZ52_18340"
     CDS             287..1747
                     /locus_tag="PKZ52_18340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNG61787.1"
                     /translation="MPRPFLLRFKPLAQAVLFTACVAFSAAASCEELQIPDLGDAGGG
                     MMSPAEEYELGQKVLRIYRSNLPTSNDPFFEVYLESLLQKLVTYSAVKDKRLELLVIE
                     DKNLNAFAAPGGIVGVNTGTFLIAQTEAQLASILSHELAHLSQRHFIRTQQKQAKANM
                     AGLVGMIGGIILGATAGPDAGMAAITAAQAGVLDARLRFSRDMEQEADRIGMETLVLS
                     GYPADAMPEMFEVMQSNSRYRTRVPEFLLTHPLTENRIADSRSRAERYPKRPPSPDVD
                     FQIAKARALFQSEESPQFAVKRFSEELKGTSLSSLTARYGLLLALIQSNQTDKAKNVL
                     VELQKDYPSLQALTVAQADIAAKSNQGAQAIELLQNALKTQPHNQLYSIRLAELLMEN
                     SRYADCEKLLQEYVKIKPKNAYSWYLLAEVHGLAGHILDVHKARAEYFILHGIYDKAE
                     IQLRNALKLVGNDFQGRARLEQRLIDVKNMQKEKLG"
BASE COUNT          510 a          528 c          453 g          406 t
ORIGIN      
        1 gtgagcaagc gggtaaattc cgctgggccg cgcagtgtac actgtgggcg cgcaatgatt
       61 aacacagcct gccaaaggct ttgcccttac agcaggagcc gcttattaac cccgcagcgt
      121 ttagtgccgg tttaataagc ccagcataat tcaagcctgt tgaatgctag caactgccta
      181 tcgccctggt gaaaggcaat taaagaatat ggctgtagcc cgccagggaa acacaacaaa
      241 ctgccagggg acaccccggc gccaatgaga gcgtgaccaa cctgtgatgc ccagaccttt
      301 tctcctgcgt tttaagccgc tggcgcaggc cgttttattc accgcctgtg tcgccttcag
      361 tgcagcggcc agctgcgagg aattacaaat tcccgattta ggtgacgcgg gcggcggcat
      421 gatgtcgccc gctgaagaat atgaattggg gcaaaaagtc ctgcgtattt accgctcgaa
      481 tctgcccacc tctaacgacc ctttttttga agtgtattta gagtctttac tgcaaaagct
      541 ggtcacctac agcgccgtca aagacaaacg cctagaactt ttagtcattg aagataaaaa
      601 cctcaacgcc ttcgccgccc ccggcggtat tgtcggcgta aacaccggta catttttaat
      661 tgcccaaacc gaagcgcaat tagcctccat tttatcccac gaattagctc acttaagcca
      721 acgccacttt attcgcaccc agcaaaaaca agccaaggct aatatggccg gtttagtggg
      781 catgatcggc ggcattatat taggggccac cgcaggccca gatgcgggta tggcggcaat
      841 tacagcggcc caagcgggag tattggatgc gcgcctgcgc tttagccgcg atatggaaca
      901 agaagccgac cgcatcggta tggaaaccct ggtgttatcc ggttaccccg cggatgccat
      961 gccggaaatg tttgaggtga tgcagagtaa ttcccgctac cgcacccggg tgccggaatt
     1021 tttactgacc cacccgctga cagaaaaccg catcgccgat tcccgcagcc gcgccgagcg
     1081 ctatcccaaa cgaccgccct cccccgacgt ggattttcaa atcgccaaag cccgcgccct
     1141 atttcagtcg gaagaaagcc cgcagtttgc cgtcaaacgc tttagcgagg aattaaaagg
     1201 caccagccta tcctcgctaa ctgctcgcta cggcttgtta ttggcgctca ttcaatccaa
     1261 ccaaaccgac aaagccaaaa atgttttagt tgaattgcaa aaagattatc cctccttaca
     1321 ggccctcacg gtggctcaag cggatattgc cgctaaaagt aaccagggcg cccaagccat
     1381 tgagctattg caaaacgctt taaaaactca accccacaat cagctctaca gtattcgctt
     1441 agccgaatta ttgatggaaa attcacgcta cgcggactgc gaaaaattgc tgcaggagta
     1501 cgtaaaaata aaacctaaaa atgcctacag ctggtattta ttagctgaag tgcacggcct
     1561 ggctggccac atcctcgacg tacacaaagc ccgcgccgaa tactttattt tgcacggcat
     1621 ttacgacaaa gcggaaattc aactgcgcaa cgccttaaaa ctggtgggca atgatttcca
     1681 aggccgcgcc cgcttagagc agcggctgat cgacgtaaaa aatatgcaga aggaaaaact
     1741 cggttaaccg gcacaaccta ttgcatgagg taatagccgg ttgcaatagg ttgccgacgg
     1801 attggcaggc aaatgagacc caaaaacagc accgcatcgt acattaggaa ttcacctcct
     1861 catttatgta gcctggatga agcacagcgc aatccgg
//
LOCUS       DAOQGS010000737         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_42750_length_1891_cov_4.181917, whole genome shotgun sequence.
ACCESSION   DAOQGS010000737 DAOQGS010000000
VERSION     DAOQGS010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42750_length_1891_cov_4.181917"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1891
                     /locus_tag="PKZ52_18345"
     CDS             <1..>1891
                     /locus_tag="PKZ52_18345"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNG61788.1"
                     /translation="QFPVSLTTENLTTDAEGLAELNLPASENPSRYVLTLFASDGAAF
                     RVKTSKEILIERGESDYRLTASRNFAAPGEKIVFSFKPLANSANNAPVRYEWIRLEDQ
                     SRGEGSLPEGADNFALPLEKSGNYSIHLRDANQLLVAGTSVTVSGPEQKTTPGSVDIL
                     FDQRQYAIGDKATALITFPEPVEEALLTLERDGVEQTALLSKGAEWLNITKLTANQYQ
                     VIIPVTSDFAPNITFSAVYSLHKDFSFQNAGIKVAKPMVTVQVSADKNEYLPGQTVNL
                     AIQTQYLDKPVAAQVALSVVDEMIYTLQPEIAPSIEEFFYHPRRNNVRTSASLAFINY
                     DLALPGSRQSLPGQRQERGVKLLERPRRDDKDTAYWAPSIQTDANGLAKVSFTLPDAL
                     TRWRITARSMTASGEVGEAKSHIRSEKPVYLKWSGPQEFRDQDQPKLGLLAFNQGKEP
                     ISAQLSVSLGEQSASSPVELKPGVSYLPLPAEIKLAAGDITAQLQAKDEPLDALAVPL
                     SLATSSWPQGRSQAVSLESQSTPISLPADARDIRLNTQTDSASLFMGATNDLVEYPYG
                     CVEQTASRLLPLAIAFGLIPSTDVEVRDRLQQILQFSRLRLIHMAGPEALFSWWGGEG
                     SDAFLT"
BASE COUNT          468 a          552 c          491 g          380 t
ORIGIN      
        1 ggcaattccc ggtgagttta accactgaaa acctcaccac tgatgctgaa ggcttagcgg
       61 aattaaacct gcccgccagt gaaaacccca gccgttatgt gctgacatta tttgccagcg
      121 atggcgccgc ctttagggtt aaaaccagca aagaaatttt aatcgagcgc ggcgagagcg
      181 actaccgttt aaccgccagc cgcaattttg cagcgcccgg agaaaaaatt gttttcagtt
      241 ttaaaccgct tgccaacagc gcgaataatg cgccggtgcg ttatgaatgg attcgtttag
      301 aagaccaaag ccgcggtgag ggcagcctgc cagagggcgc tgataatttt gccctgccct
      361 tagaaaaaag cggtaattac agcattcacc tgcgcgatgc caaccaatta ttagtggcgg
      421 gcaccagcgt aaccgtgagt gggccggagc aaaaaactac ccccggcagc gtggatattt
      481 tatttgacca acgccaatac gccattggcg ataaagccac ggcattaatt acctttcctg
      541 aacctgtgga ggaagcgctg ttaaccctag agcgcgatgg cgttgaacaa accgcgttat
      601 tgagcaaagg cgccgagtgg ttaaatatca ctaaattaac cgccaatcag taccaagtca
      661 ttattcccgt caccagcgat tttgcgccga atattacctt cagtgcggtg tacagcctgc
      721 ataaggattt tagtttccaa aatgccggta ttaaggtggc caaacctatg gtgacggtgc
      781 aggttagcgc cgataaaaac gaatatttac ccggccaaac cgtgaacctg gccattcaaa
      841 cccagtacct ggataaacct gtggccgccc aggtagccct ttcggtagta gatgaaatga
      901 tctacaccct gcagccggaa atagccccga gcattgaaga atttttttac cacccccgcc
      961 gcaataacgt gcgcaccagc gcgagcctgg cgtttattaa ttacgaccta gccctgcccg
     1021 gcagccgcca gagcctgccc ggccagcgcc aggagcgcgg cgtgaaatta ttggagcggc
     1081 cgcgccggga cgataaagac accgcctact gggcgccgag tattcaaacc gacgccaatg
     1141 gcctggccaa ggtgagtttt accctgcccg atgccctcac ccgctggcgc atcaccgccc
     1201 gcagcatgac cgcaagcggt gaggtaggcg aagctaaaag ccatatccgc tcggaaaaac
     1261 ccgtgtattt aaagtggagc ggcccccagg aatttcgcga tcaagaccag cctaaactgg
     1321 gcctgctggc ctttaaccaa ggcaaagagc cgatcagcgc gcaactgagt gtgagccttg
     1381 gcgagcagag tgcaagctcg ccggtagagc ttaaacctgg cgtcagttac ctgcccctgc
     1441 ccgccgagat taaactcgcc gccggggaca taaccgccca actgcaggct aaggatgaac
     1501 ccttggatgc ccttgcggtg cccctaagcc ttgccaccag tagttggccc cagggccgca
     1561 gccaagcagt cagccttgag agccaatcca cccccataag cctgcctgcg gacgcccgcg
     1621 acatccgcct gaatacccaa accgattcgg ccagcctatt tatgggcgcc actaatgatt
     1681 tagtggaata cccctatggc tgcgtggagc aaaccgctag ccgcctctta cccctggcca
     1741 tagcctttgg cctgattcca tctaccgatg tggaagtgcg cgatcgcctg cagcaaatat
     1801 tgcaatttag ccgcctgcgg ctgattcata tggcggggcc tgaagcgctc ttcagctggt
     1861 ggggcggcga aggtagcgac gccttcttaa c
//
LOCUS       DAOQGS010000738         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_43337_length_1876_cov_4.845140, whole genome shotgun sequence.
ACCESSION   DAOQGS010000738 DAOQGS010000000
VERSION     DAOQGS010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43337_length_1876_cov_4.845140"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..1186)
                     /locus_tag="PKZ52_18350"
     CDS             complement(407..1186)
                     /locus_tag="PKZ52_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020414979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNG61789.1"
                     /translation="MLLAKPLNDFAVFDSHLHIIDKRFALMANEGFLPAEFTCADYRA
                     AMASYPLVGGAVVSGSFQGVDQDYLIAALQELGPGFVGITQLPADAPDKQIMALHQAG
                     VRAVRFNVRRGGSADINDLTRMAQRIFDLAGWHVELYVDSSMLADMYASLIKLPAVSI
                     DHMGLSKSGLPFLLRLVERGVRVKACGFGRVDFAVAPVLKDLYKANPHGLMFGSDLPS
                     TRAPRPYQVEDFNLVVEALGEDAARKVLSENGQMFYKRLKK"
     gene            complement(1207..1632)
                     /locus_tag="PKZ52_18355"
     CDS             complement(1207..1632)
                     /locus_tag="PKZ52_18355"
                     /inference="COORDINATES: protein motif:HMM:NF036460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 domain-containing protein"
                     /protein_id="HNG61790.1"
                     /translation="MPRPSALLPVQFKQGSTMLCRRSFILCFLSLTYTLPLQALELTS
                     EAEINGLILAVEKSGCQFIRNGDAHSASDAAEHLRLKYRKAKAYAKTAEEFIDNLASQ
                     SSLTKTPYYLITPQGEKIASGVWLHRELQRLRAAAQKSP"
     gene            1657..>1876
                     /gene="rlmJ"
                     /locus_tag="PKZ52_18360"
     CDS             1657..>1876
                     /gene="rlmJ"
                     /locus_tag="PKZ52_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNG61791.1"
                     /translation="MLSYRHAFHAGNFADVLKHAALVHCLEYLVQKDNPLRYIDTHAG
                     IGSYRLDAFEPNKTQEYQQGVALLWQRPN"
BASE COUNT          498 a          507 c          442 g          429 t
ORIGIN      
        1 ccgcaaatag cttgatcagc tgcttgcggt ggtataggag agatggtatc tacgctgtag
       61 cgggcctttg cctaaattca ggatattgcc tcctaaaaag gaggagggga aaaatctgca
      121 aacccaggca ttttgctatg gcgccaggca attttgctcc ctccccttaa aaaggggagg
      181 gttggggtgg ggtttattgg tgcccgcaga atcagcaaag ttagatgaca acacgcttac
      241 attattaacc cggcagcatt tgccgcccgg ttaatagtaa aggtcgcgat ttttaatgca
      301 gtctaatagc gggtgagtca ggcaaaggtg gaatttttta cagcagcggc aatcaaccgc
      361 caacacaata actagctaaa aaataggcct aaccaccaag ttctaactat ttttttaagc
      421 gcttataaaa catctgcccg ttttcactta aaaccttgcg tgccgcatcc tccccaagcg
      481 cttccaccac taaattaaaa tcctcaacct gataggggcg cggcgctcgc gtgctgggta
      541 aatcgctgcc gaacatcagc ccatgcgggt tggccttata taaatctttt aataccggcg
      601 caacggcaaa atccacccgg ccaaatccgc aggcttttac ccgcacacct cgctcgacta
      661 aacgcaataa aaaaggcagc ccgcttttgc ttaaacccat atgatcgata ctcaccgccg
      721 gtaatttaat taggcttgca tacatatcag ccagcatgga tgaatccaca tataactcca
      781 catgccagcc cgccaagtca aaaatgcgct gggccatgcg ggttaaatcg tttatatccg
      841 ccgagccgcc ccggcgcaca ttaaaccgca ccgcccgcac acccgcctgg tgtagcgcca
      901 ttatctgctt atcgggcgca tccgccggta attgtgtgat gcccacaaac cccggcccca
      961 attcctgcag ggcggcaatt aaataatctt gatcaacccc ctgaaacgag ccggacacca
     1021 ccgcgccgcc gactaatggg tagctcgcca tagccgcgcg ataatccgcg caagtaaatt
     1081 cagcgggcaa aaaaccttca ttagccatca gcgcaaatcg tttatcaata atatgcaagt
     1141 ggctgtcaaa aacagcaaaa tcgttaagag gcttggcaag taacatcgtg tgcaccttgg
     1201 gctattttaa ggtgattttt gcgctgccgc ccgcaaccgt tgtaattcgc ggtgcaacca
     1261 aaccccgctg gcaatttttt ccccttgggg cgtaattaaa taataggggg ttttagtaag
     1321 cgagctttgg ctggctaagt tatcaataaa ctcctcagcg gttttggcat aggccttagc
     1381 cttgcggtat ttcaaacgca aatgctcagc cgcatccgaa gcgctgtggg catcgccatt
     1441 gcgaataaat tggcagccgc ttttttcaac ggcaagaata agcccattaa tttccgcctc
     1501 actggttagc tctaaggctt gtaagggcag ggtgtaggtg aggcttagga aacacaaaat
     1561 aaagctgcgg cggcagagca tggtagatcc ttgtttaaat tggacgggca aaagagcgct
     1621 gggtctgggc ataatagcgc tttttggata ccgcttatgc tcagctaccg tcacgcgttc
     1681 cacgcaggca attttgccga tgtgctcaaa cacgccgcac tggtgcactg ccttgagtac
     1741 ctggtgcaaa aagataatcc cctaaggtac atagacaccc acgcgggcat cggcagctac
     1801 cgcctagacg ccttcgaacc caacaaaacc caagaatacc aacagggtgt tgccctgctc
     1861 tggcagcggc cgaacc
//
LOCUS       DAOQGS010000739         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_43623_length_1869_cov_4.900221, whole genome shotgun sequence.
ACCESSION   DAOQGS010000739 DAOQGS010000000
VERSION     DAOQGS010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43623_length_1869_cov_4.900221"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKZ52_18365"
     CDS             complement(<1..264)
                     /locus_tag="PKZ52_18365"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNG61792.1"
                     /translation="MGRWLKLTKDEIKYAKPGRKHGAGRRLTREQEQRIKRLITDKTP
                     DQLKLAYALWTRESVRELIRSEYKIVLPIRTTGDYLKRWGMTPQ"
     gene            complement(256..342)
                     /locus_tag="PKZ52_18370"
                     /pseudo
     CDS             complement(256..342)
                     /locus_tag="PKZ52_18370"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1196..1531
                     /locus_tag="PKZ52_18375"
     CDS             1196..1531
                     /locus_tag="PKZ52_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNG61793.1"
                     /translation="MPSPNETHPLVEKIRGQGGMTARHLMRLVQEVFDRSYEQMRLKV
                     GEDNARKLKEASSHWLRHTVARMEIERGRALKDLSEDLGHASMATTDAIYVQTEHRVR
                     AQSGRERKV"
BASE COUNT          412 a          445 c          488 g          524 t
ORIGIN      
        1 ttgtggcgtc atcccccagc gcttcaaata atcacccgtg gttctgattg gcagtacgat
       61 cttgtactca gatcgaatca attccctcac cgattcgcgc gtccataagg cgtatgccag
      121 cttcagctga tcgggcgtct tgtcggttat taggcgcttg atgcgctgct cttgctcgcg
      181 cgttaaacgt cgcccggcac cgtgttttcg gcccggttta gcgtacttta tctcgtcttt
      241 cgtgagtttt aaccatcgac ctatcgtatc cgcatgaact cctaacatgt cgccaatatc
      301 ggcccttgtc attccgcgtt tccgtaggcg atgcgcctct cggcgcttct cggcgcttct
      361 cttctaaaac ttctaaagtt aattttctgg catctatttt ttccatgcag ccattgtatc
      421 agatactttt aatcgccggg ttaataaatg tgggaaaatt cggtgcaggc acgcatcagt
      481 taagctcgcg aattaatgtg gccagccatt gtggtggtgc gtcggcaggg actctgagtt
      541 gcacggcgca gatgtgtagt gaaataacca gagatgaaag gggttgcgtc gagacggcta
      601 taaatcttgg ggcatttttc gttgcagtct ttcgattttc ttcagctgct aatttatttt
      661 tttgcaaact gaagtatttg ttgttgatgc cccgagccgc gcaaaacgcg gtcgctgttt
      721 ggcctgatac ggcctgttgg tcgattaatt tttgccattg ttgtggggtg cgcctagcca
      781 taaggtttcc tctggtgaaa gacttagggt atggtggatg ggcgctcggg gctaggtggg
      841 gtttgttggt cgcttacagt ctttttcttc gtcaaaggct ttcattgttc attgcgaggg
      901 cgaaaagccc tttgttccgt ctgtcacaaa ccagatgggc ttcattttcc ctaatcaaat
      961 attggcgcga tacatctgaa gattggatca ttaaggtgcg ttttatacgt gacgtgaaag
     1021 gagggaaaga cttgccctta agatgtagaa gacaacaacg gattcacgct tctgatgcaa
     1081 ctgctccgcc atagacttgt atggatgata ctgtgaattt gtctatctat gaacgtaaat
     1141 cgcgtttagc ctcttttaat cagcagcggt tcgaagcggc caatgcggtc gcggggtgcc
     1201 ctcacccaat gaaacgcacc ccctagtaga gaaaattcgc ggccaaggag gtatgacggc
     1261 gcggcatttg atgcgcttag tgcaggaggt gtttgaccgc agttatgagc agatgcgctt
     1321 aaaagttggg gaagataatg ctcgtaaatt aaaagaggcg tcctctcact ggctgcgcca
     1381 cacggtcgcc agaatggaaa tagagcgggg gcgggccctt aaagatctct ccgaggatct
     1441 cggccatgcc agcatggcca ctaccgatgc tatttatgta caaaccgaac accgagtgcg
     1501 ggcccaaagc ggacgagagc gcaaggtgtg acgagttgaa atactccccg ccgggggcta
     1561 gatttaatcc tacacggtgg ccgaattcat acgtatggaa gcagcctgcg gccaactgag
     1621 gaaaagtttt cgatccttcg gcagtttcac atcttaaggc cacaaaaggg gttacgctgg
     1681 cagttaaatc cgctaagctg tagcaaaggt gcctgattta taacgagaaa actatgtcca
     1741 ccctgcgcgt accgcttcgt tttgttctat tcatctccct tcttcttacc gccagcgcct
     1801 ttgcttctga acccgttctc aatggtgtag ctatttactc tgaactgggt agcgagcaat
     1861 ttgtggcgg
//
LOCUS       DAOQGS010000740         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_43715_length_1867_cov_5.469647, whole genome shotgun sequence.
ACCESSION   DAOQGS010000740 DAOQGS010000000
VERSION     DAOQGS010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43715_length_1867_cov_5.469647"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PKZ52_18380"
     CDS             complement(<1..1172)
                     /locus_tag="PKZ52_18380"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNG61794.1"
                     /translation="MSAQLALSPIEAAYIAANVYFTLEGWEATYQFRAIHGSNVKGEP
                     KPRPGMASQDVIKNNVTGSGPLAMGKTGLHNSQLVSPLSGSTGTNLPGIGRTRSGFGY
                     LLQFERAGKQHLVIATRGTRPEMGYPDLLTDINISSNRHMPGAGPIHAGFFDAYNSIK
                     PQLLQLEASIKNADVVHCVGHSLGGAVANLVAIHCAQLGAEVKLYTFGAPRVGLNTAL
                     YDKVLVNYLGEENIYRVSHSLDPIPMIPVAPYIHVLPSIKDANNFFIGSPINSISLEN
                     HDTAKYIQSVEGRSWDDVRSVKLKEGYLNKQYFNQWRASDNWLKQAVGVSVNATMSLL
                     QRVFQGLIDSVGVGLKDVATLIDLLVVAIQRGVEIFQVSKSYIAKFLSDCAKMFGYA"
BASE COUNT          513 a          469 c          427 g          458 t
ORIGIN      
        1 gcgtagccaa acattttggc gcaatcggat aaaaacttag cgatatagga ttttgacacc
       61 tggaatatct ccaccccgcg ctgtatcgcc accactaaga ggtcaatgag ggtcgccaca
      121 tccttaaggc ccacccccac cgaatcaatc agcccctgga atacccgctg cagcagcgac
      181 atagtggcat ttaccgacac ccccaccgcc tgttttaacc aattatcgct ggctcgccat
      241 tggttaaaat attgtttatt taaatagcct tctttgagtt ttacactgcg cacatcgtcc
      301 cagcttcttc cctctacgga ttgaatatat ttagcagtat cgtggttttc taaactgatg
      361 gaattaatcg gcgagccgat aaaaaaatta tttgcatctt taatgctggg caacacatga
      421 atataaggcg ccacaggaat catcgggata gggtctaaac tgtgggagac gcggtagata
      481 ttttcttccc ccaggtaatt aaccaacacc ttatcgtaca gggctgtatt caacccaaca
      541 cggggcgcac cgaaggtata gagttttacc tcggcaccta attgcgcgca gtgaatagcc
      601 actaaattag ccacagcgcc acctaggctg tggcccacac aatgcactac atcggcgttt
      661 ttaatactcg cttccaactg cagcaactgc ggtttaatgg aattataggc atcaaaaaaa
      721 cctgcatgaa tagggcctgc gccgggcata tggcggttgc tgctgatatt aatatcggtt
      781 aataaatccg ggtaacccat ctccgggcga gtgccgcgag ttgcaattac caaatgctgt
      841 ttaccggcgc gctcaaattg caataaataa ccaaaccccg aacgggtgcg gccaatgccc
      901 ggtaaattgg taccggtaga accactcagg ggcgaaacca attggctgtt gtgcaaccct
      961 gtttttccca tggccaaagg ccctgaccca gtcacgttat ttttaatcac atcctggctt
     1021 gccatgcccg gcctgggttt tggctcgcct tttacattgc tgccgtgaat cgctctaaat
     1081 tggtaagtgg cctcccagcc ctccaaggta aaatacacat tggcggcgat ataggcggct
     1141 tcgatagggg aaagcgcaag ctgtgctgac ataatagatc cgtgtagtta agtggaaatt
     1201 aaaaaggggc aagcaaattt tttaaccaaa aattccagtt aaatattttc agaaaaaata
     1261 caaggtaaat attgaaccgt ccgcttaaaa gttttaaagc tgcattttta tttttgggcg
     1321 cgcgacatca gcctcagctc gaataaacat tcgcgcagat acaaatagct caataatggg
     1381 ttttgacgga tagtagcgac tagcctaagc ctgcgcaacg cgccctagat ggccttaaaa
     1441 ggccagtgca aaaagttcga cagttagtga ttgcaaacac taaataagcc cagcagtaca
     1501 tctgccagct gcactgcatt taccctgcgg caaaaatgat ttttttagcg cagcgccggc
     1561 cattttcgca cctttacgca gatgcgcttt tgtgacatgc atcactcaaa aaatagcgtg
     1621 attgctagga ctagcgggca ctcagttaag cccctagcca tgccgcaggt taaacgcgca
     1681 ggcgtcggca gccggtttaa atcggcagat tgtttaaatt attaacccgg cggcatttag
     1741 tgccgggtta ataatggcgg catggatgcc gccccggcgg tgcaaaccgc cgtgagctgc
     1801 cctaaaacaa ggaaatccgc gcattttcgc cagttttagg gcagcgaagg ctaaatagat
     1861 caggacg
//
LOCUS       DAOQGS010000741         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_43760_length_1866_cov_3.504141, whole genome shotgun sequence.
ACCESSION   DAOQGS010000741 DAOQGS010000000
VERSION     DAOQGS010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43760_length_1866_cov_3.504141"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1866
                     /locus_tag="PKZ52_18385"
     CDS             <1..>1866
                     /locus_tag="PKZ52_18385"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG61795.1"
                     /translation="NKTDQLAKFQPTTKDNKSLPLSLTRSVLKDSRGTIWLGHEKGLY
                     TFNPNTKTFASIPLKNAVENIKNVTIWKIIEDKKGILWLGTDGNGLMYYDPTSQTFGS
                     YTRTVSPSSLGAPVVRTVFEDREGDLWVSTFPTGLYHYNQTNNYFAYYANFIKNSAGL
                     YANTTWAFAEDAQQNIWLGLDSLGLVYFDRKNNTFSQTYNGFNFAEHKFPHTVLSLLN
                     DSHGNIWMGTWAQGVTRFNPKTLSYQHFNPNANDDDDQTDNRFVGDSVWSILEAKNGD
                     IYFGTMNQGIIQYSYATQQFTTYKNTQGGDAAPNNNVGWTFLEDANGRIWVGTNMGIN
                     IFNPTTKRFTYLQNDPEDDTSLSSNQILWLYQDSKARIWVCTIGGGLNLYRPQSNSFT
                     RIDTQNAFKNKDVQAIVEDNNGTLWIANTNTITSLNPDTQEMHTYTDKSWLQNGEFNH
                     GAAFKLSSGELLFGGANGFNILNPNKVTSNLAKPKVFFTELELLNETILPSSRNRVIK
                     GDILTTPSITLTHEDSMFSLRFTATNYRSYADNTYTYKLEGFDNQWHKQSTNNKATYT
                     HLDAGRYRFVVRAANNDNVWGDEEKSIEIIVLPAPWQTWWAYCIYATLAVGLIAW"
BASE COUNT          556 a          494 c          371 g          445 t
ORIGIN      
        1 acaacaaaac tgaccaactg gccaaattcc aacccaccac aaaagacaat aaaagcttac
       61 ccctgagtct tacgcgttcc gtactgaaag actccagagg aactatttgg cttggccacg
      121 aaaaaggcct ctacaccttc aaccccaaca caaaaacctt tgcatcaatc ccgcttaaaa
      181 atgcggtgga aaatattaaa aatgtcacca tctggaaaat catcgaagat aaaaaaggca
      241 ttttatggct gggcaccgat ggcaatgggc tgatgtatta cgaccccacc agccaaacct
      301 tcggctctta cacccgtacg gtttcgccct cctctctagg tgctccggtg gtgcgcaccg
      361 tgtttgaaga tcgcgagggc gatttatggg tgagcacctt tccaaccggt ttatatcact
      421 acaatcaaac caataattat tttgcctatt atgcaaattt tattaaaaac tcggcaggcc
      481 tctacgctaa taccacctgg gcttttgcgg aggatgcaca gcagaatatt tggctgggcc
      541 ttgacagcct agggctggtg tattttgacc gcaaaaacaa taccttttct caaacctaca
      601 acggctttaa ttttgccgag cataaattcc cccacacggt tttatctctg ctcaacgaca
      661 gccacggcaa tatatggatg ggcacctggg ctcagggcgt gaccaggttt aacccgaaaa
      721 ccctcagcta tcaacacttt aatcccaatg caaacgacga tgatgaccag actgataaca
      781 gatttgtagg tgatagtgtt tggagcatac tggaagccaa aaacggcgat atttattttg
      841 gcaccatgaa ccaaggtatt attcagtaca gttacgccac ccaacaattt actacctata
      901 aaaacaccca aggcggcgac gcagcgccga ataacaatgt cggctggact tttttagagg
      961 atgcaaatgg ccgcatttgg gtgggcacca atatgggtat taatattttt aaccccacca
     1021 ccaagcgttt tacctatctt caaaacgacc ccgaggacga taccagccta tccagcaatc
     1081 aaatcctctg gttgtaccaa gactccaaag cgcgtatttg ggtgtgcacc attggcggtg
     1141 gcctcaattt atatcgcccg cagagcaatt catttacgcg catcgacaca caaaacgcat
     1201 ttaagaataa ggatgtacaa gccatagtgg aggacaataa cggcaccttg tggattgcca
     1261 acaccaatac gattacatca ttaaaccccg acacccagga gatgcatacc tatacggata
     1321 aaagctggtt acaaaatggt gaattcaacc acggtgccgc cttcaaactc agcagcggtg
     1381 aattattatt tggcggcgcc aacggcttta atatactcaa ccccaacaag gtaaccagca
     1441 acctagccaa acccaaggta ttttttacgg agttagaatt attaaacgaa accatcctac
     1501 cttctagccg caaccgggtc attaaaggcg atattctcac cacccccagc ataaccctca
     1561 cccacgaaga ctccatgttt tccctgcgtt ttaccgccac caattaccgc tcctacgccg
     1621 ataataccta cacctataaa ctcgaaggct ttgataacca gtggcacaaa caatccacca
     1681 ataataaagc cacttatacc catttagatg ctggccgcta ccgttttgtg gtgcgcgccg
     1741 ccaataacga caatgtgtgg ggcgatgaag aaaaatccat agaaattatt gtgctgccag
     1801 cgccctggca aacctggtgg gcctattgta tttatgcgac cttggctgtc ggtttaatag
     1861 cttggt
//
LOCUS       DAOQGS010000742         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_44574_length_1847_cov_4.349330, whole genome shotgun sequence.
ACCESSION   DAOQGS010000742 DAOQGS010000000
VERSION     DAOQGS010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44574_length_1847_cov_4.349330"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..967
                     /gene="gloB"
                     /locus_tag="PKZ52_18390"
     CDS             170..967
                     /gene="gloB"
                     /locus_tag="PKZ52_18390"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNG61796.1"
                     /translation="MLNADFVIHPVPILTDNYVWILVHNQDAWIIDPGEAAPVAQFCH
                     SHGLMPKAILLTHLHQDHWAGVAPLLEHYPVPVLGPGGMPASIARLIAHQLAAGDTVS
                     MAERTWQVVALPGHTPEHIGFYLPGAPGHLSPGHLFSGDTLFSFGCGRAFYSVEQLFT
                     SVNWIKNLPANTCIYPTHEYTAANWRFARAVEPDNPHILTFAERLPHISPANPSLPVV
                     LADELNRNPFLRCGHKSVQKAAGLASGSSINCDLDVFSALRSWKNVF"
     gene            1268..>1847
                     /locus_tag="PKZ52_18395"
     CDS             1268..>1847
                     /locus_tag="PKZ52_18395"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNG61797.1"
                     /translation="MLHIRHYLLTSVVFLSACTFQTKKPGPIAQAQNPHNSTTHTPQI
                     QTPQQNPTPSSDTLVTLANNPDNANSSSDLWGRMRKGFSLNPIPANKLQNDLAFYGRY
                     PSFMNGVSQRGSRFMFYVVDELEKRKMPMEIALIPVIESAYDPKARSPGGAAGLWQMM
                     DGTGKVLGLKRTAAYDGRHDVVASTDAALDYLQ"
BASE COUNT          412 a          569 c          451 g          415 t
ORIGIN      
        1 ttactcagca tgggctgcag tgtctttagc tcctcctcca gcaacatctt acccaccgcg
       61 gtggtatacc aatcctccaa actggcctgc gtcgtgctta agggtgcaag acggtggcgg
      121 ttgcgccagc gttgaagcca tgacccgatc atacagaacc tctcaaagca tgttgaacgc
      181 ggattttgtc atccacccgg tacccattct gaccgacaat tatgtctgga tactggtgca
      241 caaccaagac gcctggatta tagacccggg cgaggccgca cccgtggcgc aattttgcca
      301 cagccatggg cttatgccta aagccatttt gctcacccac cttcaccaag atcattgggc
      361 gggtgttgcg ccgctgctgg aacattaccc agtgccggtg ttggggcctg gcggcatgcc
      421 cgcatctatt gcccggctta ttgcccacca gctcgcagcg ggtgataccg tcagtatggc
      481 cgagcgcacc tggcaggtgg tggcgctacc cggccacacc cccgaacaca taggctttta
      541 tttgcccggc gcccccggcc acctgtcccc cggccaccta tttagcggcg acacgctttt
      601 tagtttcggc tgcggccggg ctttttattc cgtggagcag ctgttcacct cggtaaactg
      661 gatcaaaaac ctaccggcga atacctgcat ctaccccacc cacgaataca ctgccgccaa
      721 ctggcgcttt gcccgcgcgg tggaacctga taacccccat atcctcacgt tcgctgagcg
      781 cctgccccac ataagcccgg ccaacccaag tttgccagtg gttttagcgg atgaacttaa
      841 ccgcaatcca tttttgcgct gcggtcacaa atctgttcaa aaagccgccg ggctggcgtc
      901 tggatcatcc attaattgtg atctcgacgt tttttctgcc cttcggtcct ggaaaaatgt
      961 gttttaattc acaaaaacaa ccccgcccag tgtggcggat gtgaaaaacc ttggttagaa
     1021 tcggttcaga cacaaagcaa agtgcggtta aaaatcgtcc acttctttgg ttttggttcc
     1081 gcttaaccgg agtcaaaagc ggtgcacacc gccgtcatga tgcgcagccg aacgggatgt
     1141 tgctgtggaa ttacctcttt tggaacatag gagttgagtg cgacacgcta ggtttttggc
     1201 tgtacgccta gagtagacct cacgctctga ttggccttta aggtcgccca acgtaggctt
     1261 tgcagttatg ttacatattc gccactattt actgacttca gtagtgtttc tttcggcctg
     1321 tacttttcag acgaagaagc cagggcccat cgcccaggca caaaatccgc acaacagcac
     1381 cacacatacc ccacagatac aaacaccgca acagaatcca acccccagca gtgacacact
     1441 ggtcaccctt gccaataacc cagataacgc caactcaagc tccgaccttt ggggccgcat
     1501 gcgcaaaggc ttcagcctta accccatccc agccaataaa ttgcaaaatg acctcgcctt
     1561 ctacggccgc tacccttcat ttatgaacgg tgtcagccag cgcggcagcc gctttatgtt
     1621 ttacgtggtg gatgaattag aaaagcgcaa aatgcccatg gaaattgcgc tgatccccgt
     1681 gattgagagc gcctacgacc ccaaagcgcg ctcaccgggc ggcgccgctg gcctctggca
     1741 gatgatggat ggcaccggca aagtgctggg ccttaagcgc acggccgcct acgatggccg
     1801 ccacgatgtg gtggccagca ccgatgctgc cctcgactac ttacagc
//
LOCUS       DAOQGS010000743         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_45123_length_1834_cov_4.386172, whole genome shotgun sequence.
ACCESSION   DAOQGS010000743 DAOQGS010000000
VERSION     DAOQGS010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45123_length_1834_cov_4.386172"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKZ52_18400"
     CDS             <1..210
                     /locus_tag="PKZ52_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HNG61798.1"
                     /translation="ARAMHVQILGGNYPGFLLALLPPGAFLVMGLLIAAKNFLDQHYA
                     RRQVQVIKIPAGSKRVRTTGAIKPE"
     gene            248..1339
                     /gene="trmA"
                     /locus_tag="PKZ52_18405"
     CDS             248..1339
                     /gene="trmA"
                     /locus_tag="PKZ52_18405"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HNG61799.1"
                     /translation="MTSSYQQQLNNKTTQLTHLLAPFYQQPIAVFASPEKHYRMRAEF
                     KIWQDYEHNSCQYAMYEPGSNRKSYTLETFPAASLGINNAMPKLMAAINGNEILRRKL
                     FQIEFLSSTDGEVLASLIYHRALDEAWANAAEQLSQALNIHIIGRARGSKKILSQDYV
                     QQRFLINGREFTYRHSENSFTQPNGPVCAQMLNWADTQARKIAQLGSCDLLELYCGNG
                     NFTLPLAQHFRKVLANEIAKISMQDAEHNLAHNKITNITLARMSSEELTQALNGAREF
                     RRLEGVNLQDYHFGCVLVDPPRAGLDAATLNFISQFPHIIYISCNHHTLSENLQQLSR
                     THRVIDAALFDQFPFTEHIETGVVLKRWA"
     gene            1525..1776
                     /locus_tag="PKZ52_18410"
     CDS             1525..1776
                     /locus_tag="PKZ52_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61800.1"
                     /translation="MSASNRAVAGPAQALHIYIENDGAAYLSPNQIAANPSLRANLLV
                     LRLMQLDNQSSLYLTRPCYGFAVNAMPATCDARYWTSAR"
BASE COUNT          493 a          474 c          408 g          459 t
ORIGIN      
        1 gcccgcgcca tgcatgtgca gattttaggt ggcaattacc ccggattttt attggccctg
       61 ttaccaccgg gggcgttttt ggtgatgggc ttattaattg cggctaaaaa ctttttagac
      121 cagcactacg cacggcggca ggtgcaagtc atcaaaatcc ccgctggcag caaacgagtg
      181 cgcaccacag gcgccatcaa accggaataa ccgccagtta ctcacctatt ttttatttgc
      241 gaatttcatg actagctctt atcagcaaca attaaataac aaaacaactc agcttaccca
      301 cctcctggca cccttttatc agcagcctat tgccgtgttt gcctccccag agaagcacta
      361 ccgcatgcgg gcagaattta aaatttggca ggattacgag cacaacagtt gccaatacgc
      421 catgtatgaa cccggctcca accgcaaatc ctacaccctt gaaaccttcc cagccgcgtc
      481 cttaggtatt aataacgcca tgcctaaact catggcggcc attaatggca atgaaatact
      541 gcgccgcaaa ttatttcaaa tcgaattttt aagcagtacc gatggcgagg tattggcaag
      601 cttaatttat caccgggcgc tggatgaagc ctgggcgaac gcagcagaac agttaagcca
      661 agcgttaaac attcatatca tcggccgcgc gcggggcagc aaaaagattt taagccagga
      721 ttatgtgcag cagagatttt taataaatgg ccgggaattc acctaccgcc acagtgaaaa
      781 ttcttttact cagcccaacg gcccggtctg tgcgcagatg ttaaattggg cggataccca
      841 agcgcgcaag atagctcaat tgggcagctg cgatttattg gaactctact gcggcaatgg
      901 caattttacg ctgccactcg cccagcattt tcgcaaagta ttggccaatg aaattgccaa
      961 aatttccatg caggatgccg aacacaattt ggcgcacaac aaaataacga atattaccct
     1021 cgcccgcatg tccagcgagg agttgaccca agccttaaac ggcgcacggg aatttcgccg
     1081 tttagagggt gttaatcttc aggattatca ttttggctgt gtattggttg acccgcctag
     1141 agcgggtttg gatgcggcca cattaaattt tatcagccaa tttccccata ttatttatat
     1201 ctcctgcaat caccatacac tcagtgaaaa tttgcagcag ttaagccgca ctcaccgcgt
     1261 gattgatgcg gcgctgtttg atcaattccc atttaccgag catattgaaa ccggtgtggt
     1321 attaaagcgg tgggcctgat ggatagcttg cgcacacagt tacaggccag tgtgcggttt
     1381 ataagggcaa tcccgttttt attcacttca ttcatgctat taaccagttg cacgcccatg
     1441 gcacagatgc aacacctgtg tacaggctgc acgccgctaa cactgagctc accactcagt
     1501 cctatttatg gatgggcacg caatatgagt gcatccaata gggctgtagc aggcccagcc
     1561 caggccctgc atatttatat cgaaaatgat ggcgcagctt atttatcccc caatcaaatt
     1621 gcagccaatc cctccctgcg cgcgaattta ttagtgctgc gcctaatgca attggataac
     1681 caaagcagcc tatatttaac acggccctgt tatggatttg ccgtaaatgc catgccagca
     1741 acctgtgatg ctcgctattg gactagtgct cggtaaagca aagaggtggt gcacgcactt
     1801 tcccaggcaa ttgatcaggc aaaaattaaa ctta
//
LOCUS       DAOQGS010000744         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_45470_length_1827_cov_1.935666, whole genome shotgun sequence.
ACCESSION   DAOQGS010000744 DAOQGS010000000
VERSION     DAOQGS010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45470_length_1827_cov_1.935666"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(514..1371)
                     /locus_tag="PKZ52_18415"
     CDS             complement(514..1371)
                     /locus_tag="PKZ52_18415"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNG61801.1"
                     /translation="MNEIELKANDSAFSIRGHTFRLDELVNPLLYDPVQQDRWATLLQ
                     TAAPYPHLNVMDWFNKDFLNLILEEFVLSKNLDWRVAQTDMVNFRRSVLGEYLGPATE
                     LYFTIVNSGWFVQLLSQISGVGDLIVDHSRYGGGMHETVKGGYFSVHSDFNRHVTTGL
                     TNKMVFITYLTPGWQPDWAGELELWDKDNKQCITKIAPNFGRSVLMLNGKGHYHGHPS
                     IWNAPAGIPRRSVANYYYVNEYAAFDRSEHYDSIYISPNQSEKFVAFVRPVIPPLIWQ
                     AIRKILGRP"
BASE COUNT          543 a          455 c          410 g          419 t
ORIGIN      
        1 gcaactgcct gaccaagtga gcaccaatat atccggcgcc gccggttact aaaatcgtca
       61 tggaaaatcc tcaatgtaca caaacaataa aacccatgcc acagctgcgg cagggggcgc
      121 cacccgttaa aactcatacc ttagtactgc aatggccagg cctaactagg caaaagggcg
      181 caaagccccg catgatacca aagggctgcg cggctgtaac acagttttgc cgatttgttc
      241 acaggcgcaa ccctcgggct gggcctgtga tgcccgcttg ttgcgataac taaatttaag
      301 tgaaggaaac ggagggcatg gccgttgtgg ccagatgggg tttttcgatt atgcggtgtg
      361 tgtcgattag gatgtgaatg gggaaagcta ataaattgat ctgagcgccc ccaaattccc
      421 ttctcttaaa gggattgcag gcgacctcca acagaacgat aggccgagca gtatccgcca
      481 atccatctgt aactcatggc taataaagga ggcttagggc ctaccaagta ttttacgaat
      541 ggcctgccaa ataagaggtg gaatcacggg gcgaacgaag gcaacaaatt tttctgattg
      601 attgggtgaa atataaatag aatcgtagtg ttcgctgcgg tcaaaagctg cgtattcatt
      661 gacataataa taattagcca cagaacgccg agggataccg gcaggtgcgt tccagatcga
      721 agggtggccg tggtaatggc ctttgccatt gagcataaga actgaacggc caaaattggg
      781 cgctattttg gtaatgcatt gtttattgtc tttatcccac agctccaact cgcctgccca
      841 gtcgggctgc caaccggggg tcaagtaggt aataaacacc attttattcg ttaaaccggt
      901 agttacatgc cggttaaaat cgctgtgcac actaaaatag ccgcctttaa ctgtttcatg
      961 catgccaccc ccgtagcgag aatgatccac aatcaaatcg ccaacaccgc tgatttggct
     1021 gagcagctgc acaaaccatc ctgaatttac aatagtgaaa tatagctcag ttgcggggcc
     1081 taaatattca cccaacacgc tgcggcggaa attcaccatg tctgtttgag caactcgcca
     1141 atccagattt ttgcttaaaa caaactcttc caaaattaag tttaaaaaat ccttattaaa
     1201 ccaatccatc acgtttaaat ggggatatgg cgctgcggtt tgcaaaagag tcgcccagcg
     1261 atcttgctgc accgggtcat acaatagggg attgaccaac tcatccagac gaaatgtgtg
     1321 gccgcgtata ctaaacgcag aatcattggc cttaagctct atctcattca aagaaactaa
     1381 cctgcctccg ccttgatcaa ttccagccgc taccatcgac caccctaaat acatcagccc
     1441 taaaaaattg ctcaaatgcc tatagctaat tacaactggc acgtttggct aaaagccaag
     1501 aattacatcc cccacatacg ctcgccactc agatgaaact ggcgtagccc atggaaggcg
     1561 gcaaataaat ggcgcccaaa accctggcaa tcgctatgcc agtgtatgca aagtgtattt
     1621 tttaccgaaa aaaacgcaga attattggca gcaaagtagc gcgcacgcaa agcagtgaaa
     1681 aataaatcga caaacgccag tattgtgtgc ggctttgcaa aaaagctttc agcataacca
     1741 ttaaatgaat aaggcaaccc cgcgtctacg ctatacacga acctgcgccc cttaacgaag
     1801 cgcaaatttg cggggcctgc actatta
//
LOCUS       DAOQGS010000745         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_45776_length_1819_cov_3.637188, whole genome shotgun sequence.
ACCESSION   DAOQGS010000745 DAOQGS010000000
VERSION     DAOQGS010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45776_length_1819_cov_3.637188"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKZ52_18420"
     CDS             complement(<1..323)
                     /locus_tag="PKZ52_18420"
                     /inference="COORDINATES: protein motif:HMM:NF017870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HNG61802.1"
                     /translation="MKKWLIVSASCVLSLAAALPMAVGLGLEPVVNLLVSGLTTQTAL
                     GLADSRFERGYANSQLSLTFKPLLDGAPQSLQLVVHFKHGPLLVGAGLELGLLGVTAQ
                     LKANPT"
     gene            complement(337..1032)
                     /locus_tag="PKZ52_18425"
     CDS             complement(337..1032)
                     /locus_tag="PKZ52_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61803.1"
                     /translation="MHTKRIVELSFLALFALGVGISLVIHQSYDQWLSQLVQASGSKV
                     AGGPLVLNDLKWQAAEQTLSLSGGQWFEQARASEAWLSLPAARWPVAADAWQGAQFHS
                     ERFVLAHLQVHLRQEGLSSNLNRLMKQVESVQITPEKLSRGKEPLLFTVDQVVFSDLQ
                     VEFTTLKHGVLRWHIEELTLTPTPRNQPFDVLLQQVTRALLAELKTQTTQQLLTLTEA
                     SQPSGDSPPSNEQ"
     gene            complement(1035..1472)
                     /locus_tag="PKZ52_18430"
     CDS             complement(1035..1472)
                     /locus_tag="PKZ52_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61804.1"
                     /translation="MWKLLGWPLAFAAAVGAYWLHVQGQKRERALQDQLQVQQQLVAQ
                     VSNRLTVVEAQYAIYRAKQEKDAKERELREREVRESDHGAPRTPVASEHPPAANRTPA
                     EPYEEPLDETREDTRPRRNASPARSIGNGINSIIDRLRDGFNN"
BASE COUNT          387 a          527 c          502 g          403 t
ORIGIN      
        1 gtcgggttgg ctttaagctg cgccgtcaca cccagcagcc ccagctctaa accggcgcca
       61 accagcagcg ggccgtgctt aaaatgcacc accagctgca aggactgcgg tgcgccatca
      121 agcagcggtt taaaagtcag gctgagctgg ctgttggcat agccgcgctc aaagcggcta
      181 tcggcgaggc ccagggccgt ctgggtcgtc aggccgctca ccaacagatt caccacgggc
      241 tccaagccca gccccacggc cataggcagg gcagcagcca ggctgagtac acagctggca
      301 cttactatta accatttttt catcgctgta gattcgtcac tgttcattgc taggcggact
      361 atcgcctgag ggttggctgg cttccgtgag ggtcagcagt tgctgggtag tttgagtttt
      421 caattcggct aataacgcgc gggttacctg ctgtaacaac acatcaaagg gctgattgcg
      481 cggtgttggc gtaagcgtta attcctcgat atgccagcgc aaaacaccat gtttaagcgt
      541 ggtgaattca acttgcaggt cgctaaagac cacctggtct accgtaaata acaggggttc
      601 tttgccgcgg ctgagttttt ccggtgtgat ctgcacagat tccacctgtt tcatcaggcg
      661 atttaaattg ctactcaagc cctcttggcg caggtgaacc tgcagatgcg caaggacaaa
      721 ccgctcactg tgaaactgcg caccctgcca ggcatcggcg gctactggcc agcgggcggc
      781 gggcaggctg agccaggcct cgctggcgcg ggcctgctca aaccattggc ctccagagag
      841 gctcagggtc tgctcggcgg cctgccattt aaggtcgttg agcactaagg gcccaccggc
      901 aaccttgctg ccgctggcct gtaccagctg acttagccat tggtcgtagc tctggtgaat
      961 gacgagggat atccccacgc caagcgcgaa tagggcgaga aaactcagtt cgacaatgcg
     1021 cttagtgtgc atgactaatt attaaaccca tcgcgcaggc ggtcgatgat ggaattaatc
     1081 ccattgccaa tactgcgggc ggggctggcg ttgcggcgcg ggcgggtatc ctcgcgggtt
     1141 tcatcgaggg gttcttcata gggttcggcg ggtgtgcgat tggcggcggg cgggtgttca
     1201 ctggccaccg gtgtgcgcgg cgccccgtga tcgctctcgc gcacctcccg ctcgcgcaat
     1261 tcgcgctctt tggcgtcttt ttcttgtttg gcccgataga tagcgtactg ggcttcaacc
     1321 accgttaagc ggttgctgac ttgcgccacc agctgctgct gcacctgcaa ttgatcctgc
     1381 aaggcacgct cacgtttttg cccctgcaca tgcagccaat aggcccccac cgctgcggca
     1441 aatgctaatg gccaacctaa cagcttccac ataacatcac ctaaagcccg ctcttattaa
     1501 aatagtgccc gcattataaa gacttagctc aacaaaagtc gcacatggca gcgcgatatt
     1561 cgcctccgac taagacctac gagtgatcca ctatagccct gctgccagcc ccatgctgaa
     1621 agcagttttg aaaactccca agccgggtgt agcgatcgct aaaaagctgc aaaagttaac
     1681 gcacctgaga ttgccatgca ccgctattcc cgcctagaat cggcatcgat gtgacgaccg
     1741 cgctggcatt taatggctat gacattgggt gataccgcta aacacagatt cggttatcac
     1801 ggggtgcaat tccatttct
//
LOCUS       DAOQGS010000746         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_45804_length_1818_cov_4.509926, whole genome shotgun sequence.
ACCESSION   DAOQGS010000746 DAOQGS010000000
VERSION     DAOQGS010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45804_length_1818_cov_4.509926"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKZ52_18435"
     CDS             <1..1041
                     /locus_tag="PKZ52_18435"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389227.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNG61805.1"
                     /translation="EAKLAATIKTAVRMLDNVIDINYYAVDTAKNSNLRHRPVGLGIM
                     GFQDALYQMRLPYSSDAAVEFADRSMEAVSYHAIAASSDLAVERGSYSSFKGSLWDQG
                     ILPIDSIALLAKNRGEKYIDQDQSQTLDWASLRAKVKAQGMRNSNVMAIAPTATIANI
                     TGVSQSIEPTYQNLYVKSNLSGEFTVANPYLVHDLKDRGLWDSVMVNDLKYYEGSLTK
                     IDRIPDDLKALYATAFEVEPRWIVDAASRRQKWIDQAQSLNLYIAGANGKKLDLTYRM
                     AWFRGLKTTYYLRALSATTTEKSTVAQGTLNAVSSDMGGGFNASAAVEAAAPAPVPKA
                     CSLDNPDCEACQ"
     gene            1240..1587
                     /locus_tag="PKZ52_18440"
     CDS             1240..1587
                     /locus_tag="PKZ52_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61806.1"
                     /translation="MNKVFRLILPLFFLASSHLQASVAQGVKIVRVHMLATPAPSGGV
                     YITINGPRSLDNCPGNDFEFYIDLNEQPRGALLYSHAMAAFKENYSVNIIGSDQCGGT
                     YNVETIKDFQVFK"
BASE COUNT          470 a          501 c          442 g          405 t
ORIGIN      
        1 gaagccaaac tcgccgccac catcaaaacc gccgtacgta tgctcgataa cgtgatcgac
       61 atcaactact acgccgtgga caccgccaaa aactccaacc tgcgccaccg cccggtgggc
      121 cttggcatca tgggctttca agacgccctc taccaaatgc gcctgcccta cagcagcgac
      181 gcagccgtgg aatttgccga ccgcagcatg gaagccgtca gctaccacgc tatagccgca
      241 agctccgacc tagcagtaga gcgcggcagc tactccagct ttaaaggctc cctgtgggac
      301 caaggcatac tgcccatcga ctccatcgcc ttgctggcta aaaaccgtgg cgaaaagtac
      361 atagaccaag accaaagcca aaccctggac tgggccagcc tgcgcgccaa agttaaagcc
      421 cagggtatgc gcaactccaa cgtgatggcc attgccccca ccgccaccat cgccaacatc
      481 accggggtga gccaatccat cgagcccacc taccaaaacc tctatgtgaa gagcaacctc
      541 tccggcgaat tcaccgtggc caacccctac ctggtacacg acttaaaaga tcgcggcctg
      601 tgggacagcg tgatggtgaa cgacctgaaa tactacgaag gctccctcac caaaatcgac
      661 cgcatcccag acgatttaaa agccctatac gccaccgcct ttgaggtaga accgcgctgg
      721 atagtagacg ccgccagccg ccgccaaaag tggatcgacc aggcgcaaag cctgaacctg
      781 tacatagcgg gcgccaatgg caaaaaactg gatttaacct accgcatggc ctggttccgc
      841 ggccttaaaa ccacctatta cctgcgtgcc ttatccgcca ccaccacgga aaaatccacc
      901 gtggcccagg gcactttaaa tgcggtgagc agcgatatgg gcggcgggtt taatgcttca
      961 gccgcggtgg aagctgccgc gcctgcacca gtgcctaagg cctgttcact ggataatcca
     1021 gattgcgagg cctgccagta attgtttttc gttttttaag agttctctag gggagagtgt
     1081 tcggtaaggc gggggtggac ccccgccgcc gggctttcac ttaattatgc atgcaagcaa
     1141 ttgcatgttt ctctctaggc ttttgaagtt gcgactgagg gcattgcggg gaagggtata
     1201 ggcgcaccca caaattcaat ctgatttttg gtaaatttaa tgaataaagt atttcggtta
     1261 atcctccctc ttttttttct agcctcgtct cacttgcaag caagtgttgc gcaaggtgta
     1321 aaaatagtcc gagtgcatat gctggcgacc cccgccccat cgggaggtgt ttacatcacg
     1381 attaatggtc ctcgctcgct agataattgc ccaggaaatg attttgaatt ttatattgac
     1441 cttaatgagc agccgagagg tgcattgtta tattcgcatg caatggcagc cttcaaggaa
     1501 aattacagtg taaatattat tggttcggat caatgtggcg gcacttataa tgttgagacg
     1561 atcaaagatt ttcaggtttt taaataacgc gtaattcgca ctaatttagc ccgtaagttg
     1621 ggcaacaatg cccaacgctc gcaatctccg caaaggaaat aatcgttact cgtaggttgt
     1681 ataagcgaag cgccatccga cgagcgaatg cgaattcaag gattaatcac agctctgcaa
     1741 agaatgtgat ttttaacttg gcggcgagac tggaagcggg gaaatttacg atgtttatgc
     1801 gcgagatgtt tttactcc
//
LOCUS       DAOQGS010000747         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_46167_length_1811_cov_2.812073, whole genome shotgun sequence.
ACCESSION   DAOQGS010000747 DAOQGS010000000
VERSION     DAOQGS010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46167_length_1811_cov_2.812073"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKZ52_18445"
     CDS             complement(<1..910)
                     /locus_tag="PKZ52_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNG61807.1"
                     /translation="MNSAVHPAFSLLSTQPVPAMNIWVEEYQHNKTGAQHIHIRADNP
                     ENVFLVALRTVPKDSTGVAHILEHTALCGSQRYPVRDPFFMMTRRSLNTFMNAFTSSD
                     WTAYPFASINRKDFSNLLGVYLDAVFFSRLDPLDFAQEGHRLEFSEAGNANSDLVYKG
                     VVYNEMKGAMSSVPSQLWQTLTKYLFPTTTYHHNSGGDPEAITDLTYEQLVSFYRTHY
                     HPSNAIFMTYGDISAFEHQSKFEELALQHFERSDHIIHVPDEKRYFAPVKVQEYYPLN
                     EDDLSEKTHVVLAWLLGQSTNLEDVLV"
     gene            1125..>1811
                     /locus_tag="PKZ52_18450"
     CDS             1125..>1811
                     /locus_tag="PKZ52_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HNG61808.1"
                     /translation="MAATAEIAISHVELLGVLWLMLAWVGYSNFARRRAKKDFCIASV
                     LHCYRKNWMRAMLTRENRISDAALLANLERNASFLASTSIFVIAGVFTLLASAEKVLD
                     MGLTIPGVPPTTTTLQLQFKIMLLLMIHVYAFFTFTWSMRQYGFCAILLGAAPVDTAP
                     DAQGSAADDYVRHVAKVIDLAGISYNEGLRAFYFSLAVLAWLLNDYVFVAAVATVVFI
                     LYRREFHSRTL"
BASE COUNT          401 a          403 c          526 g          481 t
ORIGIN      
        1 ccactaatac atcttctaaa ttggtggatt ggcccagcag ccaggcgagc accacatggg
       61 ttttttcgct caggtcgtct tcgtttagtg ggtaatactc ctgcactttt accggcgcaa
      121 agtagcgttt ttcatcgggc acgtgaatga tgtggtcgga gcgctcaaag tgctgcaggg
      181 ccagctcttc aaatttgctt tggtgttcaa aggcgctgat atccccatag gtcataaaga
      241 tggcgttgct ggggtggtag tgggtgcggt agaagctcac cagttgttca taggtgaggt
      301 cggtgatggc ttctgggtcg ccaccggaat tgtggtggta ggtggtggtg gggaagaggt
      361 atttggtaag ggtttgccag agttgagagg gcaccgaact catggcgcct ttcatctcgt
      421 tgtacaccac gcctttgtac accaagtcgg aattggcgtt gcccgcctcg ctaaattcca
      481 ggcggtggcc ctcttgggca aaatctaatg ggtctaagcg ggagaaaaac accgcatcta
      541 aataaacccc caataaattg ctgaagtctt tgcggttaat ggaggcgaag gggtaggccg
      601 tccagtcgga acttgtgaag gcgttcataa aggtgttgag ggagcggcgg gtcatcataa
      661 aaaacgggtc ccgcaccggg tagcgttggc tgccacagag cgcggtatgc tctagaatgt
      721 gcgccacgcc ggtagaatcc ttaggcaccg tgcgcagggc gacgagaaat acgttttccg
      781 ggttatctgc gcgaatatga atgtgctggg cgccggtttt attgtgctgg tactcttcaa
      841 cccagatgtt catggcgggg acgggttgtg tagaaagcag gctaaacgct ggatgcacag
      901 cagaattcat agggaaatct cttaattagg gctcgatggg gcatcatacg acgccagagc
      961 ggcaatttca gtaataatcc taaaaacctt agttgaacaa agcctaggtt gcaggctcgg
     1021 agcatcctta gtggcggttt aagagcttgc ttgggggccg ggttgaatta cgcgcgctgt
     1081 ttaatgaatg gcaaaaaatc accaataaat ctaaaggggg caaggtggca gcaacggctg
     1141 aaattgcaat tagccatgta gagttgttag gcgtgttgtg gttaatgcta gcctgggtgg
     1201 gctacagcaa ttttgcccgc cggcgagcga aaaaagactt ttgcattgcg tcggttttgc
     1261 attgttaccg caagaattgg atgcgcgcca tgttgacgcg ggaaaaccgc atcagcgatg
     1321 cggcgctgct ggcaaacctt gagcgcaatg ccagttttct cgcttccacc tccatatttg
     1381 taattgccgg tgtttttact ttacttgctt cggcggaaaa agttttggat atggggctca
     1441 ccatacccgg tgtgccgcct actaccacca cattacagct gcaatttaaa attatgctgc
     1501 tgctaatgat tcacgtatac gcctttttta ccttcacctg gtccatgcgc caatacggtt
     1561 tttgcgccat tttattgggg gctgcgccgg tagacactgc tcctgacgct caggggagcg
     1621 ccgcggatga ttatgttcgc catgtggcaa aagtgattga tttagcgggc atcagttaca
     1681 acgaaggcct gcgggccttt tatttttcgc tggcggtttt agcctggctt ttaaatgatt
     1741 atgtatttgt ggctgcggtt gccacggtgg tgtttattct ctaccggcgg gaatttcact
     1801 cccgcacatt g
//
LOCUS       DAOQGS010000748         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_46235_length_1809_cov_3.724629, whole genome shotgun sequence.
ACCESSION   DAOQGS010000748 DAOQGS010000000
VERSION     DAOQGS010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46235_length_1809_cov_3.724629"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1466
                     /gene="pyk"
                     /locus_tag="PKZ52_18455"
     CDS             12..1466
                     /gene="pyk"
                     /locus_tag="PKZ52_18455"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNG61809.1"
                     /translation="MIRRTKIVSTLGPASDEPGVLERLIVAGVNVVRLNFSHGSPEDH
                     LQRCQLVRELAAKHNVYVAVLGDLQGPKIRISRFANGPIKLAVGDKFVLDASLPKDAG
                     DQHCVGIDYKELPNDVVAGDLLLLDDGRVVFKVESVVGPKIFCEVTVGGKLSNNKGIN
                     RQGGGLTAPALTDKDLADIKTAVAIGVDYLAVSFPRSADDMHLARRLVKEAGGDIALV
                     AKIERAEAVESDEVLDDIIMASDAVMVARGDLGVEIGDAALIGVQKHIIDRARTLNRG
                     VITATQMMESMISSSLPTRAEVFDVANAVLDGTDAVMLSAETAAGQFPVETVEAMVRI
                     IVGAEKHPQSNKPLSRAMPKHMAIDQAIAQAAMHTANNMPGVKAIICLTETGSTPLLM
                     SRYGTHLPVFAFSRHENSLRRAALYRGVETIRFDSDTVNYVDANEWAVGKLVARGVVR
                     EGDLVIISKGDVANVVGGTNTLKIVEVGKPIAKG"
     gene            complement(1499..>1809)
                     /locus_tag="PKZ52_18460"
     CDS             complement(1499..>1809)
                     /locus_tag="PKZ52_18460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG61810.1"
                     /translation="REALNALQNNPPAYDLVLCDWDMPELTGLEVRKQIKQLAKFQDT
                     HFMMVTSISEAAKIREAIGQGITDYIVKPIDTATLEKKIRVALGWDLPEDDKQPQAAG
                     "
BASE COUNT          399 a          437 c          535 g          438 t
ORIGIN      
        1 tggagaaaaa aatgattaga cgtacaaaaa ttgtaagcac attgggcccg gcttcggatg
       61 aacccggtgt gttggagcgc ttgatagtgg caggagtgaa tgttgtacgc ttgaattttt
      121 cccacggcag cccggaagat catttgcagc gctgccagtt agtgcgcgag cttgccgcga
      181 aacacaatgt ttatgtggct gtgttgggtg atttgcaggg gcctaagatc cgtatttcgc
      241 gttttgccaa tggcccaatt aaattagccg taggtgacaa atttgtactg gatgcatccc
      301 tgcctaagga tgccggtgac cagcattgtg taggcattga ctataaagag ttgcccaacg
      361 atgtggtcgc cggggatctg ctgttgctcg atgatggccg cgtagtattt aaagttgaat
      421 ctgtggttgg cccgaagatt ttttgtgaag tgaccgtcgg cggcaagtta tccaacaaca
      481 agggtatcaa ccgtcaaggg ggcggtttaa cagcgccagc cctgaccgat aaagatcttg
      541 cggatatcaa aacggccgta gccattggtg tggattacct ggctgtatcc ttcccccgca
      601 gtgccgatga tatgcatctg gcccgtcgct tggtcaagga agcaggtggc gatattgccc
      661 tggtggccaa gatcgagcgg gctgaggcgg tggagagtga cgaagtatta gatgacatca
      721 tcatggcctc cgatgcggtt atggttgcgc gcggtgactt gggcgttgag attggtgacg
      781 ctgctctgat cggtgtgcaa aaacacatca tcgaccgcgc ccgcaccctc aatcgcggtg
      841 taattacggc cacccagatg atggagtcta tgatcagcag ttcgctgccc actcgcgccg
      901 aagtgtttga cgtggccaac gcggtattag atggtaccga tgcagtgatg ttgtcggccg
      961 agaccgcagc cgggcagttc ccggtggaaa cggtagaggc catggtgcgc atcatcgttg
     1021 gagcggaaaa acacccgcag tccaataagc cgcttagccg cgctatgcca aaacatatgg
     1081 caatcgacca ggctatcgcc caggcggcca tgcataccgc caacaatatg cctggtgtta
     1141 aagccattat ttgcttgacg gagaccggct cgacaccatt attgatgagc cgttatggta
     1201 cccatctgcc agtgtttgcc ttttcccgcc acgaaaactc cctgcgccgc gctgccttgt
     1261 atcgcggtgt tgagacgatt cggtttgata gcgatacagt gaactacgtc gatgccaacg
     1321 agtgggcggt gggtaagttg gtggcgcgcg gtgtggtgcg ggaaggggat ttggtgatta
     1381 tttccaaggg cgatgtggcc aatgtggtgg gcggtactaa cacgctgaaa atcgtcgaag
     1441 tgggtaaacc catcgccaaa ggttaacaaa ccgcggctag tgcaaggctg gccgcagcct
     1501 acccggctgc ctgcggttgt ttatcgtcct ccggcagatc ccaacctagt gcgacgcgga
     1561 tttttttctc caaggtggcg gtatctatgg gcttaacgat gtaatccgtt atgccctggc
     1621 caatggcctc gcgaattttg gccgcctcgc taatggaggt taccatcata aaatgggtgt
     1681 cttggaattt ggccagctgc ttaatttgct tgcgtacttc aagaccggtt aattccggca
     1741 tatcccaatc acacagcact aaatcatagg cggggggatt attctgcagc gcattgagtg
     1801 cctcgcggc
//
LOCUS       DAOQGS010000749         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_46236_length_1809_cov_3.707526, whole genome shotgun sequence.
ACCESSION   DAOQGS010000749 DAOQGS010000000
VERSION     DAOQGS010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46236_length_1809_cov_3.707526"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..668)
                     /locus_tag="PKZ52_18465"
     CDS             complement(54..668)
                     /locus_tag="PKZ52_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008295739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNG61811.1"
                     /translation="MVNPRAARLLLGMLLCLWLQACATATPTNRQNICALFEEKSGWY
                     KDAKKAADKWQSTVPIIMSIMYQESAFVAKARPARTKILWVIPGPRPSDAYGYPQARD
                     NTWDWYQEKTGHYSARRYDFADAADFIAWYNFQSNQMAGIALTDAFNLYLAYHEGQGG
                     FKRGTYKGKNWLLNTAKTVAARTANYDAQLKNCQAELERKRGWF"
     gene            complement(797..>1809)
                     /gene="ligA"
                     /locus_tag="PKZ52_18470"
     CDS             complement(797..>1809)
                     /gene="ligA"
                     /locus_tag="PKZ52_18470"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNG61812.1"
                     /translation="GTITPVARLEPVFVGGVTVSNATLHNKDEINRLGVCIGDSVIVR
                     RAGDVIPQIVAVVLERRPSDAKPIEFPKHCPCCGSDLYEDQTDVAIRCIAGNLCPAQN
                     KEAIKHYASRQAMDIEGLGEKLVEQLVDAGLVRSIADIYELTRAQLAGLERMGEKSAD
                     NLLAAIAESKKTTLARFLFSLGIREVGQATAVNLANYFKDINALQAASLEDLQKVPDI
                     GPVAAGFIVEFFAEEKNRQLVADLLARGIDWPAITTNTSAPLAGQTWVLTGSLSQLTR
                     DEAKERLQALGAKVAGSVSAKTHCVVAGEAAGSKLAKAQELNIRILDENELLALLNNP
                     PS"
BASE COUNT          431 a          488 c          425 g          465 t
ORIGIN      
        1 cctgaccaga gcttcgccgc ctacattttt cagaggttcc ctaaaaacct cggttaaaac
       61 caaccgcgct tgcgttctaa ctcagcttgg caatttttta attgtgcatc gtaatttgca
      121 gttcgcgcgg caacggtttt agcggtattg agcagccaat ttttgccttt ataggtgccg
      181 cgtttaaagc cgccctgccc ttcgtggtag gccaaataca aattaaacgc atcggttaat
      241 gcaataccgg ccatttgatt ggactgaaaa ttataccaag cgataaaatc cgccgcgtcg
      301 gcaaaatcgt agcggcgggc gctgtaatgg ccggtttttt cttgatacca gtcccaggta
      361 ttatcccgcg cctgggggta accataggca tcggacgggc gcgggcctgg gatgacccag
      421 agaattttcg tgcgggcggg ccgggctttg gctacaaagg cggattcttg atacatgata
      481 gacataataa tcggcacggt agattgccat ttatccgctg cttttttcgc atccttgtac
      541 caaccggatt tttcttcaaa taaagcacaa atattttgcc ggttggtggg cgtggcagtg
      601 gcgcaggcct gcaaccataa acagagcaac ataccgagca ataagcgcgc cgcgcgtggg
      661 ttaaccatca cttaaacctt cccattaaat taaggaacct ctgaaaaatt accttactcg
      721 ctctggcgtc gttgaatttg ccataaaatt gccaacccta gtggtttttg acctttgtgt
      781 tcgcgcccga gaaaaactaa ctgggcggat tattcagcag cgctaaaagt tcgttttcat
      841 ccaaaatgcg gatattaagt tcctgggctt tggcaagctt ggaacctgcc gcctcccccg
      901 ccactacaca gtgggtttta gccgatacac taccggccac cttagcgccc aaagcctgca
      961 aacgctcttt agcctcatcg cgggttaatt ggctgaggct gccggttaat acccaggttt
     1021 gcccagccaa aggtgcacta gtgttggtgg tgatggctgg ccaatcaata ccgcgcgcaa
     1081 gcagatccgc cactaactgg cggttttttt cctcggcaaa aaattccacg ataaatcccg
     1141 ccgccaccgg gcctatatcc ggcacctttt gtaaatcctc caggcttgcc gcctgcagcg
     1201 catttatatc tttaaaataa ttggccaaat tcacagccgt tgcctggccc acttcgcgaa
     1261 tacccaagga gaataaaaaa cgcgccagag tagttttttt actctctgca atggccgcca
     1321 ataaattatc ggcggatttt tcccccatgc gttcaagacc tgccaattgc gcgcgggtga
     1381 gttcataaat atcggcaata ctgcgcacca aaccggcatc caccaattgt tccactaatt
     1441 tttcccccag gccttcaata tccatggctt ggcggctggc gtagtgtttg atggcctctt
     1501 tattttgcgc cgggcacaga ttaccggcga tgcagcgaat ggctacatcc gtttgatctt
     1561 cgtataaatc gctgccgcag caggggcagt gtttgggaaa ttcgataggt tttgcatcgc
     1621 tggggcggcg ctctaacacc accgcaacaa tttgcggaat gacatccccc gccctgcgca
     1681 caattacgct atcgccaata cacactccta aacgattaat ttcatcctta ttgtgtaagg
     1741 tggcgttact caccgttacg ccgccgacaa ataccggttc taaacgggct acgggggtaa
     1801 tagtaccgg
//
LOCUS       DAOQGS010000750         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_46303_length_1808_cov_2.088990, whole genome shotgun sequence.
ACCESSION   DAOQGS010000750 DAOQGS010000000
VERSION     DAOQGS010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46303_length_1808_cov_2.088990"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="PKZ52_18475"
     CDS             complement(<1..1783)
                     /locus_tag="PKZ52_18475"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNG61813.1"
                     /translation="MAGQGVIEQAEKETRIQQASDRLSIDWKSFDVNGDERVQFIQPN
                     SSSIAFNRILSNKGSLIQGRIDANGQVVLINPNGLIFTEGASVNAGGLIASALQMNDQ
                     DYLNGKFTLNALEGSEGRVINSGLLNAATGGNISLLGQSVENKGLISAQLGSVSLAAG
                     KEAVLTFEPNGLVGVRVTEAVLQKDLGVDAAVVNSGDIKAEGGKVLITASTSKDIFSK
                     AVNIGELTAAKSAVVNDDGSFTLDAGADIKNTGTVDVASHAVAGEVVLIGDNISHQGR
                     IIADSETTNAGRVELVAKNKSEIKDDGVVTAVAQSQGKGGDIKILGDKVGVFNSAKVD
                     ASGANGGGQVLLGGDRTGENPLIKNAQFLFVGDKTEIKANATQSGDGGRLIAFAEDAA
                     RIYGQFNAAGTAEGYGGFVETSGLNHLDLESTPNVGGALNKNGLWLIDPANISIVTGS
                     TESTDGAGDINNPYTNPIENGKYSTASIGANIIRDALNKGGDVYIKTGRSTSNITVAD
                     TDGNITVNATSITTSSANNSTLYLDAGRNITFNDANNYQFSLSNSNGKSLSLGLFAGK
                     DIDFKSATIITNGGDIKIGSSQVPIGGT"
BASE COUNT          471 a          486 c          322 g          529 t
ORIGIN      
        1 cggtgccacc aataggaact tgggaactgc ctattttgat atcgccgccg ttagtgataa
       61 ttgttgcaga cttaaaatct atatctttac cggcaaataa ccccaaggaa agagacttgc
      121 cgtttgaatt agaaagcgaa aattgataat tattggcatc attaaaggtt atattacgcc
      181 ccgcatccaa gtacagagtc gagttattag cgcttgaggt agtgatgctt gttgcattta
      241 cggtaatatt gccatcggta tccgccacgg taatatttga ggtggatcta cctgttttta
      301 tataaacatc gcctccctta ttcagggcgt cacgaattat atttgcacca atgcttgcag
      361 ttgaatactt accattttcg attggattag tataagggtt gttaatatcc cccgctccat
      421 ctgtagattc agtcgatccc gtgacaattg aaatattagc tggatcaatg agccacaacc
      481 catttttatt cagcgcgcca cccacattag gggtagattc aagatctaaa tgattaagac
      541 cagatgtctc aacgaaccca ccatacccct ccgctgtgcc tgcggcattg aactgcccat
      601 atatacgggc ggcgtcttcc gcaaatgcaa ttagtctccc cccatcgcca ctttgagtcg
      661 cattggcttt gatttcagtt ttatccccaa caaataaaaa ttgtgcattt ttaataagcg
      721 gattttcacc agttcggtca ccgccgagta gaacctgccc tccaccattt gcaccagatg
      781 catctacttt ggcgctatta aatacaccta ccttatcacc caatattttt atgtcgccgc
      841 ctttaccttg cgattgcgca actgcagtta cgactccatc atctttgatt tcgcttttat
      901 tttttgcaac aagctcaacc cggcctgcat tggttgtttc actatcggca atgatacgcc
      961 cttggtggct aatattgtca ccaataagta caacctcgcc ggccacagca tgggaagcta
     1021 cgtcaacggt accggtattt ttaatatccg cacccgcgtc caaagtaaaa ctaccgtcat
     1081 cattcacaac ggcactttta gctgcagtta actcaccaat atttacggct ttactaaata
     1141 tgtctttact cgtactagca gttattaaaa ctttaccgcc ttcagctttt atatcaccgc
     1201 tgtttactac agccgcatcc actcccaaat ctttttgtaa aaccgcttcg gttaccctca
     1261 ctccaaccaa tccattgggc tcaaaggtta atactgcctc cttcccagcc gccaagctca
     1321 cgctacctaa ttgcgcgctt attagcccct tgttctctac actctggcca agcaaactaa
     1381 tattcccgcc cgtcgcagcg tttaacaaac cagagttaat aacccggcct tcactacctt
     1441 ccaaagcgtt taaagtgaat ttaccgttta aataatcttg gtcgttcatc tgcaacgcag
     1501 aagcaattag tccacccgca ttaacactag ccccttcagt gaaaattaat ccattagggt
     1561 taattaatac cacctgccca ttggcatcaa ttcgcccctg aattaacgag cctttattgc
     1621 ttagaatccg gttaaatgca attgatgagc tattgggctg aatgaattgc accctctcat
     1681 caccattaac atcaaagctt ttccagtcga tgcttaggcg gtcactggcc tgctgaatgc
     1741 gggtttcttt ttccgcctgt tcaataacac cttgcccagc cacaacctgg ccgccctgcg
     1801 gagcggca
//
LOCUS       DAOQGS010000751         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_46606_length_1800_cov_4.135244, whole genome shotgun sequence.
ACCESSION   DAOQGS010000751 DAOQGS010000000
VERSION     DAOQGS010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46606_length_1800_cov_4.135244"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKZ52_18480"
     CDS             <1..1033
                     /locus_tag="PKZ52_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNG61814.1"
                     /translation="YIAFGEINWVAGLFYGIKPAVTAIVVQAAHRIGSRALKNNLLWG
                     IAAAAFVAIFALNVPFPLIVILAAMIGYIGGRVAPGYFNAGGGHGKAEKSFGAALIDD
                     HTPSPYHARFGWAHLAKISSVGAILWAVPMGILIATQGWEGTLTQMAWFFTKAALLTF
                     GGAYAVLPYVYQGAVGTYAWLTPTQMIDGLALGETTPGPLIMVVAFVAFVGGYVKAVF
                     GPDNLFMSGAVAAVLVTWFTFLPSFIFILAGGPLVESTHGEMKFTGPLTAITAAVVGV
                     ILNLALFFGYHVLWPNGFSGSLDWMSVMIALGAALALFTFKRSIIQVIGLCALIGLAV
                     KLSGINTLV"
     gene            1042..1488
                     /locus_tag="PKZ52_18485"
     CDS             1042..1488
                     /locus_tag="PKZ52_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNG61815.1"
                     /translation="MKWITRERPKIDRIACPWLITRFIDKEPEFLYVPAHQVLSEAQR
                     TGAIPFDVPGVELTHVGEECSFDTFIKRYGLTDPALLDLAVIVRGADTDRHELAPQCA
                     GLLAISLGLSHNFQDDQALLAQGLVLYDALYAWCSQVRTETHSWSN"
BASE COUNT          368 a          490 c          484 g          458 t
ORIGIN      
        1 ttatatcgcc ttcggggaaa ttaactgggt ggccggtttg ttttacggca ttaaacccgc
       61 cgtaaccgcc atcgtggttc aggccgccca caggatcggg tcgcgagcgc tcaaaaacaa
      121 tctgctctgg ggtatagccg cagcggcatt tgtggctatt tttgcgttga atgtgccgtt
      181 tccccttatc gtcatccttg ccgccatgat tggctatatt ggcgggcgag ttgcccccgg
      241 atactttaat gccggaggcg gccatggcaa ggcggaaaaa tccttcggcg ccgccctgat
      301 cgacgaccat acaccaagcc cctaccacgc ccgctttggc tgggcacacc tggctaaaat
      361 aagcagcgta ggcgctattc tctgggcggt gcccatgggg attctgattg cgacacaggg
      421 atgggaaggc acgctaaccc aaatggcctg gttttttacc aaagcggcgc tgctgacgtt
      481 tggcggtgcc tatgcggtac ttccctatgt ttatcagggt gcggtaggca cctacgcctg
      541 gttaaccccc acccaaatga ttgatggctt ggccctcggc gaaaccactc ccggcccgct
      601 cattatggtg gtggcctttg tggcttttgt cgggggttat gtaaaagccg tttttggccc
      661 ggataacctg tttatgtcgg gcgccgtggc ggctgtgctg gtaacctggt ttaccttttt
      721 accctccttt atttttatcc tagcgggcgg ccccttagtg gaatccaccc atggcgagat
      781 gaaatttacc ggccccctca ccgccatcac cgccgccgtt gtcggggtaa ttttaaacct
      841 cgccctattc ttcggttatc acgtgctctg gccaaacggc tttagtggca gcttggattg
      901 gatgtccgtc atgattgcct taggcgcagc ccttgccctc ttcacattca agcgctcgat
      961 catccaggtc attggcctgt gcgcgctgat cgggttggcg gtaaaactgt ctggtatcaa
     1021 cacactggtg tgaggtgagt gatgaagtgg attacccgtg aacgcccgaa gatcgaccgc
     1081 attgcatgtc cctggttgat cacgcgtttt atcgataaag agccggagtt tttgtatgta
     1141 ccggcgcatc aagtattaag cgaggcacag cgcacgggtg cgattccctt tgatgtgccc
     1201 ggtgttgagc tcacccatgt aggtgaagag tgcagcttcg atacctttat caaacggtat
     1261 ggcctgactg atcctgcgct gctggatcta gccgtcattg tgcgcggtgc ggatacggat
     1321 cgacatgagc ttgcacctca gtgcgcaggc ctattggcca tatcactggg gttatcccat
     1381 aacttccagg atgaccaggc cttattggcg cagggcttgg tgctctatga cgccctctac
     1441 gcatggtgca gccaggtgcg aactgaaacc cacagttgga gtaactaatt tcgctaccca
     1501 gcgcgcagct ggtcgattaa tcgcgtgaga aaattcactc attcaacagc gcttgattaa
     1561 gtctgaaaaa cccgctccgc agccacaaag gatgtacctg tgccgttcaa aatagtcaca
     1621 gattgagact aaaccctaag catcctaaaa cctaacaacc gcacttgaac tctactgcgg
     1681 cggctgaggc gctgaatatg ggcgccttag gcagcctcgc tacgagttca actgcggttt
     1741 ttaggttaag gaacctctga aaaattacct tactcgctct ggcgtcgttg aatttgccaa
//
LOCUS       DAOQGS010000752         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_46957_length_1792_cov_3.995970, whole genome shotgun sequence.
ACCESSION   DAOQGS010000752 DAOQGS010000000
VERSION     DAOQGS010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46957_length_1792_cov_3.995970"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..649)
                     /locus_tag="PKZ52_18490"
     CDS             complement(344..649)
                     /locus_tag="PKZ52_18490"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNG61816.1"
                     /translation="MSVFAKRLKEIRKLRGLSQEKLGTLAGIDEASASGRMNHYERGR
                     HEPDFTMVERLASVLNVPEAYFYAKDDEMAQLLQLWPRLKKAQRQAISTLFEELAAP"
     gene            972..1484
                     /locus_tag="PKZ52_18495"
     CDS             972..1484
                     /locus_tag="PKZ52_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61817.1"
                     /translation="MQYIYPIQPEQVLSDPRIILHFLADDPTTQTGKHYVTLLEGGYF
                     IFVTTYQSAIDAFGNNDRFWLGGTQLELPVMGLKPLITALERHFEHTHHLSLRSQVVF
                     KYAKTIEGEPLSLSRDYVLPGYSLKNHARKVHFSWAGDEWVQDLRLADSVLFEQGYYA
                     ALRQLAEDYG"
     gene            1588..>1792
                     /locus_tag="PKZ52_18500"
     CDS             1588..>1792
                     /locus_tag="PKZ52_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61818.1"
                     /translation="MRNPAPYPLQPNLPLIQHHLCLPVGVQELAHIHDFSIEGRTWSF
                     LRAHQKLILLIHYRQRSGGDTQYG"
BASE COUNT          446 a          438 c          421 g          487 t
ORIGIN      
        1 cccaactgga ggcagttggg gttttttatt gtgtgttaat gcgtatttct gcggagattt
       61 gcaggttttc gaggattttt tccaattcca attggcgcct attttttaaa catttcggcg
      121 tttttttctc tctcctcggt attattctcc gaattctcct gtgcaccaaa ataggaagtc
      181 cgctaacacc tcaaattcag cccctttaaa atcaatgggt tacaagcgga cttttcaagc
      241 ggtttgattg tgtcattggt aggggaatct attagacatg cactgcagag gggacccagc
      301 taggttgccg cacctctcac ctcaggccaa aaatggactc ccgctaagga gctgctaact
      361 cctcgaacaa tgtgctgatg gcttgtcgct gtgccttttt gagtcgtggc caaagttgga
      421 gcagttgggc catctcgtcg tctttggcat aaaaataggc ttctggaacg tttaatacgc
      481 tggccaagcg ctcgaccata gtgaagtcgg gctcatgcct tccgcgttca taatgattca
      541 ttctcccgct ggcagaggct tcgtcgatac ctgccagcgt tccaagtttc tcttgcgata
      601 atcccctcag tttccgaatt tctttcaatc gtttcgcaaa aacggacatg gtttgccctc
      661 gtttaggaag gcagcgatct tcagtggatg cgtaagcttt tttacctagg attcctaagt
      721 tttagaatct ctggtaacct agtgcggaaa ttgtgtgacg gcgggataag tgggtagacg
      781 cctgaactca tgggttttat acaagagtgc aaagctgtgg ctcgatatga tatttcctag
      841 agcgtgctgg ggtgtctgct caagatgggc attgataaac tgcaatacac ccctcacgcg
      901 cacaggtgct aactagctga attaaaagcg aacgatcaaa cggcattgat aacaaggagt
      961 agacaacagg cgtgcaatat atttatccaa tccagcctga gcaggtttta agtgacccaa
     1021 gaatcatcct gcatttcctg gcagatgacc ccaccaccca aaccggcaag cattatgtga
     1081 cgttgttgga aggcggttac ttcatctttg tcaccaccta tcaaagcgca attgacgcgt
     1141 ttggaaacaa cgacaggttt tggcttgggg gtacccagct tgaattaccg gtcatgggcc
     1201 ttaagccatt aattaccgct ctagagcgcc actttgaaca cacgcaccac ctaagcttgc
     1261 gcagtcaagt ggtcttcaaa tatgccaaaa ccatcgaggg tgaaccacta agcctcagcc
     1321 gcgactatgt attgcccgga tactcgttaa aaaatcatgc ccgcaaagtg catttttcat
     1381 gggctggtga tgaatgggta caagacctac ggttggccga tagtgtgtta tttgagcagg
     1441 gttattatgc cgcgctgagg cagttggccg aagactatgg ctagcgcgcc ctacaacttc
     1501 tttaccaacc aaggtcctca tcgaccttag aacactaaaa agctttgata actgcagtaa
     1561 accatggact taaaatggga gcattaaatg cgcaacccgg ccccataccc cctgcaaccc
     1621 aacctgccat taattcaaca ccacctctgt ttgcctgtgg gtgtgcagga gctggcacac
     1681 atacacgact tcagtattga aggccgtacc tggtcgtttt tacgcgctca ccagaagcta
     1741 attttgttga tccattatcg ccagcgttca gggggagata cacaatatgg ct
//
LOCUS       DAOQGS010000753         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_47328_length_1784_cov_3.008676, whole genome shotgun sequence.
ACCESSION   DAOQGS010000753 DAOQGS010000000
VERSION     DAOQGS010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47328_length_1784_cov_3.008676"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKZ52_18505"
     CDS             complement(<1..466)
                     /locus_tag="PKZ52_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG61819.1"
                     /translation="MRLTCIQLAGFKSFVEPTALNFVGNRTAIVGPNGCGKSNVIDAV
                     RWVMGESSAKHLRGDAMTDVIFNGSNSRRPQGFASVELIFDNSEQRLSGQYAGLKSLA
                     IKRRLSRDGDNQFFINGEKCRRRDVTDLFSGTGLGPRAYAIIEQGTIARLIDA"
     gene            complement(906..>1784)
                     /locus_tag="PKZ52_18510"
     CDS             complement(906..>1784)
                     /locus_tag="PKZ52_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61820.1"
                     /translation="DKALIIGDLNAYAQEDPVFELTSHGYIDQVGAFNSFGYSYVFDG
                     AAGRLDHAISSAALSPWVAGVKHWHINADEPSIIDYNLEFKQPACPACGPDYYTPSVY
                     RSSDHDPVIVGLNLNDIDGDGLTDAQEASLGTNPQDLDSDDDGLADGSEDANHNGQVD
                     AGETNPTRPDTDGDGLQDGTERGITTAVADPDGAGPLLGTNVSLFQPDLEPAFTTNPT
                     NADTDGDGSPDGIEDNNHNGRLDAGEYDPLSASSHPEPATSRQVPSLPLWAMVLMGGL
                     LVGQAGRVKRRQGAVS"
BASE COUNT          325 a          508 c          570 g          381 t
ORIGIN      
        1 tggcgtcgat aaggcgggcg atggtgccct gttcgatgat ggcgtaggcg cgggggccga
       61 ggccggtgcc gctgaacaga tcggtgacgt cccggcggcg gcatttttcg ccattgataa
      121 aaaactggtt gtcgccgtcc cggcttaagc ggcgcttgat ggcgaggctc ttaaggcccg
      181 cgtattggcc gctaaggcgc tgctcactgt tatcaaagat cagctccacg ctggcaaacc
      241 cctggggcct gcggctattg gagccgttga agatcacatc ggtcatggca tcgccgcgca
      301 gatgcttggc ggaggactcc cccatcaccc agcgcaccgc gtcgatcaca ttggatttgc
      361 cgcagccgtt gggccccact atagccgtgc gattgccgac aaaattcagc gcggtgggct
      421 caacgaagga cttaaagcca gcaagctgta tgcacgttaa ccgcataggt ggggtggaga
      481 taaataaaat tgagggttat ctgcgctctt tgcggggcgc cagcgggggg cggggattgt
      541 acaggtttgt ggattgctta acagcggccg tgaaactttt ttgcccgccg gggggagtgc
      601 gattgagact ggccagctac taatacaccc aaagcgactc ggccctagat tatggaattg
      661 cccgcgattt ggcgggggcc ttatgagtag cctggatgaa gcgcagcttt ttacctattt
      721 ttgcgtatgg gagtgcagcg ggctgctaac aaaaaacagt acagcgacct accaccccac
      781 cccttcgggg cacccctcca caggagggga attttagtct ctgcccatgg cgagaaacag
      841 ccgccgactc ttaccttatt gcctccgcag gaggggaatt ttgcactgca cctcgctccg
      901 ggggactagc tcaccgcgcc ctgccgccgt ttaacccggc ccgcctgccc caccaacaac
      961 ccgcccatta acaccatggc ccataggggc aggctgggca cttggcggct ggtggcgggc
     1021 tcggggtggg agctggcgct tagggggtcg tattccccgg cgtccaagcg gccattgtgg
     1081 ttgttatctt caattccgtc ggggctgccg tcgccgtcgg tatcggcatt ggtggggttg
     1141 gtagtgaagg ccggttccaa gtccggctgg aatagactga cattagttcc cagcagtggc
     1201 cctgccccgt ctgggtcggc cacggcagtc gtgatgcccc gctcggtgcc atcctgcagg
     1261 ccatcaccat cggtgtctgg tcgggtgggg ttggtttcac cggcgtccac ctggccattg
     1321 tggttggcgt cttcgctgcc atcggccagg ccgtcatcgt ctgaatccag atcctgcggg
     1381 ttagtgccga ggctcgcctc ttgggcgtcg gtgagcccat cgccgtcgat atcattgagg
     1441 tttaagccga caatcaccgg gtcgtgatcg gaggagcgat aaacactcgg ggtgtaatag
     1501 tccgggccgc aggcggggca cgcgggctgt ttgaactcca ggttgtaatc gatgatggag
     1561 ggttcatcgg cattgatatg ccagtgttta acccccgcca cccaggggct taaagccgca
     1621 ctgctgatgg cgtggtcgag gcggcccgcc gcgccgtcga acacatagga ataaccaaag
     1681 ctgttaaagg cccctacctg gtcgatatag ccgtgactgg tgagctcgaa caccgggtct
     1741 tcctgggcgt aggcgttgag atcgccgatg atcagcgcct tatc
//
LOCUS       DAOQGS010000754         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_47734_length_1775_cov_4.896512, whole genome shotgun sequence.
ACCESSION   DAOQGS010000754 DAOQGS010000000
VERSION     DAOQGS010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47734_length_1775_cov_4.896512"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1034
                     /locus_tag="PKZ52_18515"
     CDS             291..1034
                     /locus_tag="PKZ52_18515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG61821.1"
                     /translation="MTPSAHILVVDDDPDIGQLIKDYLQQFNLACTPVLSGIAMDAAM
                     AAAPFDLVILDVMLPGEDGFSLCRRLRAQSSLPILMLTARGEAFDRVVGLELGADDYV
                     VKPFEPRELLARVRSLLRRSQMQAPAAEQAQPEAVAPPLAQARQHFAGWSLDLTQRQL
                     TSPDGVVVALSTAEFKLLQVFLHKPGRVLSREQLLDEARGRNIEAFDRSIDLLVSRLR
                     HKLGEDPKDPKLIRTLRGEGYIFTARPSP"
     gene            1031..>1775
                     /locus_tag="PKZ52_18520"
     CDS             1031..>1775
                     /locus_tag="PKZ52_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNG61822.1"
                     /translation="MTQRLLATLIAAVRPLLPQSLFGRLFGLLALAIVASHLLFAVQI
                     NLRDGHSRPERRPPPGFMEQAPGQPEPPSRAERRPPRPREGEPASGWFWLGLVGQCLL
                     VLGIARWGSRMLTQPIEQLAEGALALGENLQRPPLAEAGPIEARLAIRGFNQMQIRLK
                     HQLAERSQFLLAVSHDLRTPLTRMRLRYEQLPAGELQEKLLQDWQDLSRLVASTLDYV
                     RGQDPQGAPVPVDVEALLGAMVEDRVELGQ"
BASE COUNT          321 a          532 c          576 g          346 t
ORIGIN      
        1 ggcaaagggc accatggaaa cggcgggctc tgctgctgtg gcggaagcgg gtaggtgcaa
       61 agtggcgagg gccgccacag gttgctgttg aattaaggcg gaaatagcgg cggcgtcaga
      121 atcgtgcatg gggcaactca atggggagaa aatggcccat tattcgcctg caacgggcgg
      181 cgctcaagcc ttaacccctg tgtatcaaag ctgatacaaa ccccacacac tcggcgtggc
      241 ggccatgcac tatgcttggc ggcaagcgtt tactcttttt gcgagcccct atgaccccaa
      301 gcgcccatat tttggtggtg gatgacgacc ctgacatcgg ccagcttata aaggattacc
      361 tgcagcaatt taacctcgcc tgcacgccgg tgttgtccgg cattgctatg gatgcggcca
      421 tggcggcggc gccttttgac ctggtgatac tggatgtgat gctgccgggg gaggatggct
      481 ttagcctgtg ccgtcgcctg cgcgctcagt ccagcctgcc gattttaatg ctcaccgcta
      541 ggggggaggc ctttgaccga gtggtggggc tggagctggg ggccgatgat tatgtggtga
      601 aacccttcga acccagggag ttattagccc gggtgcgcag cctgttgcgg cgcagccaga
      661 tgcaggcacc cgctgccgag caggcccaac ctgaggcggt tgcgccgccc ttggcccagg
      721 cccggcagca ctttgcgggc tggagcctgg atctcaccca gaggcaatta actagcccgg
      781 acggggtagt ggtggcgctc tccacggcgg aatttaaatt gttgcaggtg tttttacaca
      841 agccgggccg ggtgctcagc cgcgagcagc tgttggacga agcccgcggc cgcaatattg
      901 aagcctttga tcgcagtatc gatttattag tctcccgcct gcgccacaag ctcggcgagg
      961 accctaaaga ccccaagctc atccgcaccc tgcgcggtga gggttatatt tttaccgccc
     1021 ggccaagccc atgacacagc gtttattagc cacccttatc gcagcggtgc gaccattgct
     1081 gccgcagagt ttattcggcc gcttattcgg cctattggcg ctggccattg tagccagcca
     1141 cttactgttt gccgtgcaga taaatctgcg cgacgggcac agccgccccg agcgcaggcc
     1201 gccgccgggt tttatggagc aggcgcccgg ccagccggaa ccccccagcc gagcggagcg
     1261 gcggccgccc aggccccgtg agggcgagcc tgccagcggc tggttttggc tgggactggt
     1321 gggccagtgt ttgctggtat tgggcatcgc ccgctggggc agccgcatgc tcactcagcc
     1381 catcgagcaa ctggctgagg gcgccctggc cctgggggaa aacctacagc gcccgccctt
     1441 agcggaggcg ggccccatcg aagcgcgcct ggccattcgc ggttttaacc agatgcaaat
     1501 caggcttaaa catcagctgg ccgagcgcag ccaatttttg ctggccgtct cccacgacct
     1561 gcgcacgccc ttaacccgca tgcgcctgcg ctacgagcaa ttgcccgctg gggagctgca
     1621 ggaaaaactc ctgcaggatt ggcaggatct ctcccgcctg gtggctagca ccctggatta
     1681 cgtgcgcggc caagacccac agggcgcgcc ggtgccggtg gatgtggagg ctctgctggg
     1741 ggctatggtg gaggatcgtg tagagctggg ccagg
//
LOCUS       DAOQGS010000755         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_47830_length_1773_cov_4.306752, whole genome shotgun sequence.
ACCESSION   DAOQGS010000755 DAOQGS010000000
VERSION     DAOQGS010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47830_length_1773_cov_4.306752"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1051)
                     /locus_tag="PKZ52_18525"
     CDS             complement(53..1051)
                     /locus_tag="PKZ52_18525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNG61823.1"
                     /translation="METLFSSLDWTLLRSFIALIDTGNLQAAARKINASQPTVGRHIA
                     LLEQQLGKRLFDRRARQLIPTEAALLIAQYARNMEANACAIDRVLKGAPVAESKNLTL
                     TAPQGLAWGYIPIILPRLHARHPNVLFTICASNQVMNLARGEADIALRMFRPQQESLI
                     ARRVGTIKLGLFAHQRYVNKKGLPKTLSDLKNHQLISFDNKYSKNKFCELLACDEREL
                     VVRFQSDDLYVQIQMLMQGLGIMLLPEIFAAADGNLVRCLPDAPIMPMEIWLAMHEDL
                     RTCPTTLDIYHQLADSIETYLNQNTSLYFDALFIDSLHADERSAAGHPALAEPSYC"
     gene            complement(1241..>1773)
                     /locus_tag="PKZ52_18530"
     CDS             complement(1241..>1773)
                     /locus_tag="PKZ52_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNG61824.1"
                     /translation="VKLTPLPFLIKAVAAALRAEPTFNVSMHADGEHIVQKDYVHIGM
                     AVDTPNGLMVPVIRDADKKGIYQIAQESNALAKKAREGKLTPKEMQGGCFTISSLGAI
                     GGKGFTPIVNCPEVAILGVSRAETKPVWNGKEFAPRFMLPLSLTYDHRAINGGDAGRF
                     FTYLNAVLADVRRLLL"
BASE COUNT          465 a          434 c          438 g          436 t
ORIGIN      
        1 ttggcaaatt cgcctagcca gcgcttcgcc gcctacattt ttcagaggtt ccttaacaat
       61 aggacggctc ggcaagcgct gggtgccctg cggcgctgcg ctcatctgcg tgtaaagaat
      121 ctataaataa ggcatcgaaa tagagtgatg tattttgatt taagtatgtt tcgatagagt
      181 ctgccaactg gtggtatata tccaaagtag tgggacatgt gcgcaaatct tcatgcatgg
      241 ccaaccaaat ttccatgggc ataataggcg catccggtag gcagcgcact aaattgccgt
      301 cggcagcggc aaatatttca ggcagcagca taatgccaag cccctgcatt aacatttgta
      361 tttgtacgta taaatcgtca ctctgaaacc gcaccaccaa ttcacgttca tcgcaggcta
      421 acaattcgca gaatttattt ttagaatatt tattatcgaa actgatgagc tgatgatttt
      481 ttaaatcaga tagggtttta ggtagcccct ttttgttgac ataacgttga tgggcaaaca
      541 ggcccaattt tattgttcct acgcggcggg caattaatga ttcttgttgc gggcgaaaca
      601 tgcgcagggc aatatccgcc tccccccgcg ccagattcat aacctggttg ctggcacaga
      661 tggtaaataa cacattggga tggcgggcgt ggagcctggg taaaataatc gggatataac
      721 cccaggctaa accctgcggg gcagtgaggg ttaaattttt actttctgcc acaggagcgc
      781 ccttgagcac ccggtctatt gcgcaggcat tcgcctccat gttgcgcgcg tattgcgcaa
      841 tcaatagggc cgcttcggtg gggataagtt gacgggcgcg gcgatcaaat aaacgcttcc
      901 ccagctgctg ctctaacagc gcaatatgcc gccccaccgt cggctggctg gcattaattt
      961 tacgggctgc cgcttgtaaa ttgccggtgt caattaacgc aataaatgag cgcagcagtg
     1021 tccagtcgag cgaactaaat aatgtctcca taaaaactcc caagtgaaaa gctgcgggag
     1081 catatagggc tagaaaggcg ctgccaagat gcaggtattg agtacaaccg gctgatgtgt
     1141 tggtaactca agatagattt gccacctatg gctggaccta ctaaaagcag gcaaaaaaaa
     1201 cccgccatta aggcgggttt agggcttaag ccgctggcaa ttaaagcaac aagcgcctta
     1261 catccgccaa taccgcattt aaataggtaa agaagcggcc agcatcaccg ccgttaatgg
     1321 cgcggtgatc ataggtgagg ctcaagggca gcataaaacg cggcgcaaat tctttgccgt
     1381 tccagaccgg tttggtttcc gcccgggaaa cacccaaaat agccacttca gggcaattca
     1441 caatcggcgt aaaacccttg ccgccgatgg ctccgaggct agaaatggta aaacagccgc
     1501 cctgcatttc tttcggcgtt aatttacctt cgcgagcttt tttcgccaat gcgttggatt
     1561 cctgggcaat ttggtaaatg ccttttttat ccgcatcgcg aatcaccgga accattaaac
     1621 cgttcggtgt atccacagcc ataccaatat gcacataatc tttttgcact atgtgctcgc
     1681 catcggcgtg catggagaca ttaaacgtag gctccgcgcg cagagctgca gcgacggctt
     1741 taattaaaaa tggcagcggg gttaatttaa cgc
//
LOCUS       DAOQGS010000756         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48129_length_1767_cov_4.362150, whole genome shotgun sequence.
ACCESSION   DAOQGS010000756 DAOQGS010000000
VERSION     DAOQGS010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48129_length_1767_cov_4.362150"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKZ52_18535"
     CDS             <1..243
                     /locus_tag="PKZ52_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61825.1"
                     /translation="VGKQQGQWRGLELKDLTPGYEPSANKLKFKYYQTLSGEIVLPEG
                     FEPEYTELEARAPGKAPVNKRIKWNGQSLAANQPDT"
     gene            252..641
                     /locus_tag="PKZ52_18540"
     CDS             252..641
                     /locus_tag="PKZ52_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNG61826.1"
                     /translation="MGFANTHTLIAKGTKILGDLYFAGDLQIEGEIKGNVYAEAGSQA
                     RVVVAEHGTVVGEIHGTQVVINGQVTGDVYSTKHLELAAKAQVIGNVYYQLIEMVKGA
                     QVNGSLVRQDAAPSPSGKKTASEKSPA"
     gene            718..1071
                     /gene="erpA"
                     /locus_tag="PKZ52_18545"
     CDS             718..1071
                     /gene="erpA"
                     /locus_tag="PKZ52_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNG61827.1"
                     /translation="MSAIQTYTPSPLLITANAVNKVRSLVEEEGNPALKMRVYVTGGG
                     CSGFQYGFSFEEEAAEDDTLIEKDGITLLLDPMSYPYLVGSQLDYEEGLQGAKFAINN
                     PNATSTCGCGSSFSI"
     gene            complement(1129..>1767)
                     /locus_tag="PKZ52_18550"
     CDS             complement(1129..>1767)
                     /locus_tag="PKZ52_18550"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNG61828.1"
                     /translation="NRCVLNLGGIANLTYLPTSADCTGFDTGPGNGLMDAWIARIQNQ
                     PFDEGGQWAAGGRLNEGLLVRLLQHPYYTLPPPKSTGKEEFTLAWLESVLAEYEAQCG
                     QAMAAADVQRTLLELTAQSIATALEHFCPACEEIYACGGGVCNPLLMERIAAIAKRPL
                     FTTQALGVAPQWVEAMAFAWLAKRCLEGMPGNLPSVTGAAQAAVLGAIYPAG"
BASE COUNT          425 a          519 c          480 g          343 t
ORIGIN      
        1 gtgggcaaac aacagggcca atggcgcggt ctagaattaa aagacctcac ccccggctat
       61 gagccaagcg ccaacaaact caaatttaag tactaccaaa ccctcagcgg tgagatagtg
      121 ctgccggagg gctttgagcc tgaatatacg gagctggaag cccgtgcgcc gggcaaagcg
      181 cccgttaata aacgcatcaa gtggaacggc cagagcctcg ccgccaatca acccgacacc
      241 taggagccac catgggcttt gctaataccc acaccctgat cgctaaaggc accaagattc
      301 tcggcgacct ttactttgcc ggtgatttac aaatagaggg ggagattaaa ggcaatgtgt
      361 acgccgaggc cggtagccag gcccgcgttg tggtggccga acacggcacg gtggtgggtg
      421 agatccacgg cacccaggtg gtaattaacg gccaggtcac gggggatgtc tactccacca
      481 aacaccttga gctggccgct aaagcccagg tgataggcaa tgtctattat cagttaatcg
      541 aaatggtaaa gggtgcccag gtgaatggca gcctagtgcg ccaggacgcg gcgccatcgc
      601 catcgggtaa aaaaaccgct agcgagaaat cccccgccta gggagaaatt cggtaatact
      661 tgactgaatt actagggcaa ctggataatc caggccgtaa tctgcgaggt acattttatg
      721 tccgctattc aaacctatac cccgtcaccg cttttgatca ctgccaatgc cgtgaataag
      781 gtgcgcagcc ttgtggaaga agagggcaat ccggcgctga aaatgcgcgt gtacgtgacg
      841 ggcggcggct gttcggggtt ccagtatggt ttcagctttg aagaagaagc cgccgaagac
      901 gataccctga tcgaaaaaga cggtatcacc ctgcttctgg accctatgag ttacccctat
      961 ttggtgggct cccagctgga ttacgaagag ggcttacagg gggccaaatt cgccatcaat
     1021 aaccccaacg ccacctcaac ctgcggctgc ggctcttcat tctctatcta gagctctatt
     1081 gaatacttca cactgcgggg tgaagacacc ccgcctcctg ctccagtatt aacccgccgg
     1141 ataaatcgcc cccagcaccg ccgcctgggc ggcccccgtc acgctgggta gattcccagg
     1201 catgccttcc aaacagcgtt ttgccaacca ggcaaaagcc atggcctcaa cccactgcgg
     1261 cgccacaccc agggcctggg tggtgaacag gggccgcttt gcgatggccg caatacgctc
     1321 catcagcaag ggattacaca cgccgccgcc acaggcataa atctcttcac aggccgggca
     1381 aaagtgttca agggccgtgg cgatactctg cgcggtcagc tccagcaggg tgcgctgcac
     1441 gtccgccgcc gccattgcct gcccgcactg cgcctcatat tccgccagta cagattcaag
     1501 ccaagccagg gtgaactctt ctttaccggt gcttttaggt ggcggcagcg tgtaataagg
     1561 atgctgcagc aagcgcacaa gcaagccttc atttaatcgg ccgccagccg cccattggcc
     1621 gccttcatca aacggttggt tttgtatgcg cgcaatccac gcatccatca agccgttacc
     1681 cggcccagta tcaaaccctg tgcaatcggc ggatgtgggc aggtaggtaa ggttggcaat
     1741 gccacctagg ttgagcacac agcgatt
//
LOCUS       DAOQGS010000757         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48132_length_1767_cov_4.113902, whole genome shotgun sequence.
ACCESSION   DAOQGS010000757 DAOQGS010000000
VERSION     DAOQGS010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48132_length_1767_cov_4.113902"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PKZ52_18555"
     CDS             <1..1162
                     /locus_tag="PKZ52_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNG61829.1"
                     /translation="MVAAVLFVVSAWGAGISTSSLEFILYRGLGGLAVGAASVMAPAY
                     ISEVAPARYRGSLATIQQVAIIGGLTAAFLSNYLLAKLSGSALHPLWFGYETWRWMFW
                     VAVAPAVVFLFALFLIPESPRYLVAKQKNDQALKVLQKLYGDTAGQQKLSEISASLAA
                     DHHQPRLSDLIDSTTRKIRPLVWVGVGLASFQQLVGINVVFYYGAVLWQSVGFSENDS
                     LFINVVSGSLSIAACIAALLLVDKVGRKPLLWIGSVGMAITLTLCTIAFSTASLDAKG
                     NLSLSESMGALALIAANLYVIFFNFSWGPIMWVMLGEMFPNQIRGSGLAVAGFSQWTC
                     NFGITMTFPILLASVGLSGAYGFYAFCAALSVVFVIKFVHETKGVELENMKG"
     gene            1344..>1767
                     /locus_tag="PKZ52_18560"
     CDS             1344..>1767
                     /locus_tag="PKZ52_18560"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNG61830.1"
                     /translation="MNISALVRRRYATKAFRADRQIPTEQLAQLCDLLRFAPSSTNSQ
                     PWHFVLAHSPAGKAQVATAAHGTYASNASKINNSSLCVVFCAKTDLSDDYLAAVLNQE
                     EQDGRFAPNPAFKQSVDAGRKMYADLHRQQLNDAQHWME"
BASE COUNT          351 a          492 c          466 g          458 t
ORIGIN      
        1 aatggtggcg gcggttttgt tcgtcgtctc cgcttggggg gccggtatat cgaccagctc
       61 gctggaattt attttatacc gcggcctcgg cggcctggcg gtgggggcgg ccagtgtgat
      121 ggcgcccgcc tacatcagcg aagtagcacc cgcccgctat cgcggcagtc tggccactat
      181 ccagcaggtg gccatcatcg gcggtttaac cgcagcgttt ttaagtaact atttactggc
      241 gaaactctcc ggctccgcgc tgcatccgct ctggttcggt tatgagacct ggcgctggat
      301 gttttgggtg gccgtagccc ctgcggttgt tttcttattt gcgttgtttt taattcccga
      361 aagcccgcgt tatttggtgg ccaaacagaa aaacgatcag gctctaaaag tcttgcaaaa
      421 actctacggc gacactgcgg ggcaacaaaa actcagtgaa attagcgcgt ctttagccgc
      481 cgaccatcac cagccgcgcc tgtcggatct aatcgattcc accactcgca aaatccgccc
      541 cctcgtctgg gtgggcgtag gccttgcctc cttccagcaa ttggtgggta ttaatgtggt
      601 gttttactac ggtgcggtgc tgtggcaatc ggtagggttt tcagagaacg actccctgtt
      661 tatcaacgta gtgtccggct cgttaagtat tgccgcctgt attgccgccc tgctgttggt
      721 ggacaaggtt ggccgcaagc cactgctgtg gattggctcg gtgggcatgg cgattaccct
      781 aaccctctgt accatcgcgt tttctaccgc ctccttggat gccaagggta atttgagcct
      841 gtctgagagc atgggcgccc tggcactgat cgcagccaat ttgtatgtga tcttttttaa
      901 cttctcctgg gggccaatta tgtgggtcat gctcggcgag atgttcccca accagattcg
      961 cggctctggc cttgcggtgg ctgggttttc acagtggacc tgcaattttg gcatcaccat
     1021 gaccttcccg attctgctcg cctccgtcgg cttgtccggc gcctatggct tttacgcgtt
     1081 ttgtgcggcg ctctcggtgg tgtttgttat caagtttgtg cacgaaacca aaggtgttga
     1141 gctggagaac atgaaaggct aagcacccgg ttgcattgct ccgtaggggg caatgcaacc
     1201 tctttctgca ttttaattta cccctgctaa ttgcaaaccc cagttatcct tcccccgccc
     1261 tactcgcccg ccgcatagac taaaatccct ccgttctttt aattcgcagc gcaattttat
     1321 ttgcacttaa tttagggtcg attatgaaca taagcgctct tgtgcgtcgc cggtacgcaa
     1381 ccaaagcatt ccgtgccgac cggcaaatcc ccaccgaaca gcttgcacaa ctgtgtgact
     1441 tgctgcggtt tgcgccctcc agcaccaact cacaaccttg gcattttgtg ttagcacaca
     1501 gccctgcagg caaggcccaa gtggctaccg ccgcacacgg cacctatgcc agtaacgcca
     1561 gcaaaattaa caactcaagc ctatgtgtgg tgttctgtgc gaaaacagat ttgagcgatg
     1621 actacttggc cgctgtgtta aatcaggaag aacaggatgg ccgctttgcg cccaacccgg
     1681 cgtttaaaca gagtgtggat gccggccgca aaatgtatgc ggatctgcac cgacagcaat
     1741 taaacgacgc ccagcattgg atggaaa
//
LOCUS       DAOQGS010000758         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48173_length_1766_cov_5.251899, whole genome shotgun sequence.
ACCESSION   DAOQGS010000758 DAOQGS010000000
VERSION     DAOQGS010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48173_length_1766_cov_5.251899"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..1565)
                     /gene="accA"
                     /locus_tag="PKZ52_18565"
     CDS             complement(618..1565)
                     /gene="accA"
                     /locus_tag="PKZ52_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNG61831.1"
                     /translation="MNLNYLSFEQSIAELEGKIEELQLVGSDNRLNISDEIARLRDKS
                     IKLTESIYANLTPWQIVQVARHPHRPHAVDYIERIFDDWDELHGDRHFGDDKAIVAGV
                     ARLDGKPVMVIGEEKGRSVQDKVARNFGMPKPEGYRKALRLMEMAERFKLPILTLIDT
                     PGAYPGIDSEERGISEAIAQNLAVMSRLRTPIICTVIGEGSSGGALAIGVGDQLNMLQ
                     YSTYFVISPEGCANIIWKTVDKAPLAAEAMGVTSKVLESLGIVDETIPEPLGGAHRDL
                     DAMAAKLKERLIAQLAELEKVDLDTLIEKRYARLMSYGN"
BASE COUNT          457 a          493 c          414 g          402 t
ORIGIN      
        1 tggcgccgcg aaattgcagc agtttcaaaa taccgatgat ctgtttgacg gcatcaatac
       61 aggactttag cgggtcaccc tcctgggaac cgctaaaaaa caactcgagg tcgcgggcgg
      121 cctgctcgat ggtcgcaacc agttcgttgc gcaccatatt cagggtctga aggttgacag
      181 aatccgcagt gttcacactt tatttcccgt tgcatagcct tggccgagcc aaaacgctga
      241 ctgaagtggt gcctttttga acccaaatcc acacgcccac ccgctgcgac aataatctgc
      301 ataaccctat aggccaatgg gacaaaaccc gcctcagccg cgcggacagc tgggaaaaag
      361 ccaagattct caaggcttta atagcctctt gagcaggttt taggattcaa aagacaatga
      421 ttgaaacgcg gcaatgctaa cgaattccgg cgccactgtc accgcgttac cccattgata
      481 ataggcaaaa aactgcagaa ctctagcagc ttggcggtgg ttaagaccct cacaggaagg
      541 gtttgccgga atatgcgggc tgtgtaccaa cccgctatag aaacgtgcta gaaacacacg
      601 caaggaagac ttaactgcta attaccgtag ctcatcaagc gcgcatagcg cttctcaatc
      661 agcgtatcca agtcaacttt ttcaagctca gccagctgtg cgatcaagcg ctcttttagc
      721 ttcgccgcca tagcatccaa atcccggtgg gcgccgccca gcggctcagg gatagtttca
      781 tcaactatgc ctaaactctc taacaccttg gaggtaaccc ccatggcttc agcggccaaa
      841 ggtgctttat cgacggtttt ccaaataata ttggcgcagc cttcaggaga gatcacaaag
      901 taggtcgagt actgcagcat attgagttga tcccccaccc caatcgctag agcgccaccg
      961 gaggagcctt cgccgatcac ggtgcaaata attggcgtgc gcaggcggga cattaccgcc
     1021 aggttctgag caatagcctc gctgatgccg cgctcttccg agtcaatgcc ggggtaggcg
     1081 ccgggggtgt caattagagt gagaataggt aatttaaacc gttccgccat ttccattaag
     1141 cgcaaggctt tgcggtaacc ctcaggcttt ggcataccga aattacgtgc caccttatcc
     1201 tgcacactgc ggcctttttc ctcgccgatc accatcaccg gtttgccatc caagcgcgcc
     1261 acaccggcca ctatggcttt gtcgtcacca aagtgtcgat caccgtgcaa ctcgtcccaa
     1321 tcatcaaaaa tccgctcgat ataatccacc gcatggggcc tatgggggtg acgggccacc
     1381 tggacaattt gccaaggagt taaattggcg tagatgctct cggttaactt aatggattta
     1441 tcccgcaagc gggcaatttc atcactgata ttcaaccggt tatcagagcc cactaattgc
     1501 aactcttcga tcttgccttc taactcagct atagattgct caaaacttag gtaatttaaa
     1561 ttcatagtga ttaatcgtcg tacggcagag gctgccccaa tagcaaaaag tcgcgcagtg
     1621 taccattaac cttaaacaag cttaaggcca gttttttagt gcagggcggc aaatccctcc
     1681 ttcccgccat acaccagcgt taccgcatca acaccataca aactgcgcaa ttacgccagc
     1741 agtaacgctg gtgtatggcg ggaagg
//
LOCUS       DAOQGS010000759         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48260_length_1764_cov_4.765360, whole genome shotgun sequence.
ACCESSION   DAOQGS010000759 DAOQGS010000000
VERSION     DAOQGS010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48260_length_1764_cov_4.765360"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKZ52_18570"
     CDS             complement(<1..789)
                     /locus_tag="PKZ52_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61832.1"
                     /translation="MWWGAFTDSFSGGNTQFEYKNESQTAIECTMGDAGSFNMCGNNC
                     VFADHNVVSHERLLSDLAFASEISPQISLDLSGYSGVWIDIDYRGPANFIHLSLQNHE
                     PALNLPDPNRQFRPQSVGIATSELHKPVYVRLKEFKVSDWWVKQFALHRTESDTHFNR
                     IRAIAIEIKEQPAHSTHYVDIKSITFVGEWISKENFYLAIIVAFVTLLGLEGAFRIHA
                     LYNRHRAAQKSLDALNEHNQKLQSVAFKDELTQLLNRRAIHEIIN"
     gene            complement(1079..>1764)
                     /locus_tag="PKZ52_18575"
     CDS             complement(1079..>1764)
                     /locus_tag="PKZ52_18575"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNG61833.1"
                     /translation="LRIDTGVFKASGAIVLQLGWKTLWNKNSDDEAVEQTLPQLGAGD
                     SGTCLHGRLLEKTTQPPEHFTDASLLAAMTGIARFVSDPLLKSILKETDGIGTEATRA
                     GIIELLFRREYLTRDKKYILATGKGKALIAALPSVASTPDMTAHWERKLSAIASGELD
                     YTDLIGPLSIELKALIDTAAPVHLPAQEHTAYAAAAKTKKRRPTPAAKKPASRKRAAK
                     KTNTKRVQR"
BASE COUNT          407 a          392 c          467 g          498 t
ORIGIN      
        1 gttgataatt tcatgtatcg cgcggcgatt aagcagttgc gtcagctcat ctttaaaggc
       61 cacagattgc aatttttgat tatgctcatt gagcgcatcc agtgattttt gtgccgcgcg
      121 gtgcctgttg taaagcgcgt gaatccgaaa tgcaccctca agccctaata aagtgacaaa
      181 cgcaacaata atggccaggt aaaaattttc tttgctaatc cattcgccca caaaggtaat
      241 cgattttatg tctacataat gtgtgctgtg tgcaggctgt tctttaattt caatggcgat
      301 ggcccgtatg cggttgaaat gggtgtcgga ctctgtgcgg tgcaaagcaa attgttttac
      361 ccaccaatcg ctgactttaa attctttaag gcgaacataa acgggtttgt gaagttcgga
      421 ggtggcgatg ccgacactct gagggcgaaa ttgccgatta ggatcgggta aatttaaagc
      481 cggctcatga ttttgtaagg ataaatgaat aaaatttgcg ggccctcggt aatctatatc
      541 aatccacact ccagagtaac cgctgagatc gagggaaatc tgcggtgaaa tttcactggc
      601 aaatgccaag tcagataata atcgctcgtg gcttaccaca ttgtggtcgg caaacacaca
      661 gttgttgccg cacatattaa atgaaccggc atcgcccatg gtgcattcaa tggcggtttg
      721 gctttcgttt ttatattcaa attgggtatt accgccagag aatgaatcgg taaaagctcc
      781 ccaccacaca tttgcaccgg gatagatttg caattttttt tgtgggatat aaggaaagga
      841 gagtagtgca agagctgtaa tgagtaccag cgcaatcttc gccgcagtca agtgttgcgc
      901 tagtgcagat gttttgggaa gcatgctggg cgatccgttg agtgccgact gctcgaagta
      961 tagttgctac agcggcagct accctaaaaa tctccgttag cgcgagcaga tctctaccgc
     1021 cgtcggcaaa ctgaccgctt gggcaggtgt tatagggagg tgggttttcg gctgccttct
     1081 atcgttgtac ccgttttgta ttagtttttt tggccgcgcg ttttcggctt gctggtttct
     1141 tcgccgcagg cgtcgggcgg cgtttttttg ttttggcagc ggcggcatag gctgtgtgtt
     1201 cctgtgcggg cagatggacc ggtgcggcgg tatcaatcag cgcctttaat tcaatactta
     1261 atgggccgat taaatctgtg tagtcaagct ctcccgaagc tatggccgag agtttgcgct
     1321 cccagtgggc ggtcatatcc ggtgtgctgg ctacactagg cagggcggca ataagggctt
     1381 tgcctttgcc ggtggcgagg atgtattttt tatcgcgggt taaatattcg cggcggaata
     1441 ataattcgat gatgcctgcg cgagtggcct ctgtgcctat gccatcggtc tcttttagaa
     1501 tcgattttaa taaagggtcg gatacaaacc gggctatgcc ggtcatggcg gcgagcaaac
     1561 tagcatcggt aaagtgctcg ggcggctggg tagttttttc cagtaaccta ccgtgtaaac
     1621 aggtgccgct atcgcctgca cctaattggg gtagagtttg ctccaccgct tcatcgtcgc
     1681 tatttttatt ccatagggtc ttccatccca actgcaacac tatagcgccg ctggctttaa
     1741 acacgcctgt atcaatgcgc aata
//
LOCUS       DAOQGS010000760         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48334_length_1763_cov_3.175059, whole genome shotgun sequence.
ACCESSION   DAOQGS010000760 DAOQGS010000000
VERSION     DAOQGS010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48334_length_1763_cov_3.175059"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PKZ52_18580"
     CDS             <1..1248
                     /locus_tag="PKZ52_18580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNG61834.1"
                     /translation="TALILLPDGDGWGWEKDQSCVVTANTRTDFTPSPQDKGAPQCQY
                     ASNDPNQDGWGWENKALCQVGPSTLQKPQAAPATPPSSIDASAYDGFLVSAIYEGRAD
                     YISLHLRDADTVTTEAKGTEKFMSALVKTADLRNGPVFVGFNAFSVDEWWVFEQNPPR
                     HQVGAEFKHLVQVGINHTSYGIHRLQLQEIKLVGQRISDETYLMSIMLVWIAYLVAEA
                     GFRYRYLTTSMQGNTEHLLTLERDTSRLEYEKLQLKSRSRTDPLTGVSNRNGLNLHMQ
                     ELYAGDRLPSGTAVAVLDIDHFKTLNDTHGHDAGDQVLVNFARVVTSAIRARDVFVRW
                     GGEEFVLLFDDTTPEKLHFTCEKLRQLVADSRLVSGSDQQVTVSIGAALTRQIEGFET
                     VFKRADAALYRAKRQRNTVEYEA"
     gene            1280..>1763
                     /locus_tag="PKZ52_18585"
     CDS             1280..>1763
                     /locus_tag="PKZ52_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61835.1"
                     /translation="MALGLIVSLVVFLGHFFIPTKRLALVPEGHYAIKLYGFPDEQLG
                     ISASWLDKEQNHWLCNYKPSHGYGCGWDVTISTTPGKGMDWTQYNKLELTMRYKGPAT
                     RLRVFLRNFNPAYANADDFESFKTMNMSFPVEDVSKPILVDLREFQVVAWWFAGKRAR
                     L"
BASE COUNT          426 a          462 c          476 g          399 t
ORIGIN      
        1 acagctttga tccttctccc tgatggtgac ggctggggtt gggagaagga tcaaagctgt
       61 gtggtaaccg ctaacactcg caccgacttt accccaagcc cacaggataa aggcgcaccc
      121 cagtgccagt acgcgagtaa cgaccccaat caagacggct ggggctggga aaataaagcg
      181 ttatgccagg ttggccccag caccctacaa aaacctcagg ccgcacccgc caccccgcct
      241 tcgagcattg atgctagcgc ctatgatggc tttttagtct cggcgattta tgaagggcgc
      301 gcggattaca tcagcctgca tctgcgcgat gccgatactg taaccaccga agccaagggg
      361 accgaaaaat ttatgtctgc gctggtcaaa acggcggatc tgcgcaacgg gccggtgttt
      421 gtggggttta acgcgtttag cgtggatgaa tggtgggtgt tcgagcagaa cccgcctcgc
      481 catcaagttg gcgccgagtt taaacacctc gtgcaagtgg gtattaatca caccagttac
      541 ggcattcacc gtttgcagct gcaagaaatt aagctggttg ggcaacgcat cagcgatgag
      601 acctacctga tgagcattat gctggtatgg attgcctact tagtggctga ggcaggattt
      661 cgctaccgct atttaacgac ctccatgcaa ggcaacacag agcatctgtt gaccttggag
      721 cgcgatacca gccgtttgga atacgaaaaa ctgcagttaa aaagccgctc cagaactgac
      781 ccactcactg gggtgagcaa ccgcaatggg ctcaatcttc acatgcaaga gctatatgcc
      841 ggtgatcggc tacccagcgg gaccgctgtt gctgtgttgg acatcgacca ttttaaaacc
      901 ctcaatgata cccatgggca tgatgctggc gaccaggtgt tagttaattt cgccagagtt
      961 gtcaccagcg ccattcgcgc acgggatgtt tttgtacgtt ggggcggtga agaatttgtc
     1021 ctgctatttg acgataccac cccggaaaaa ctgcacttca cctgtgaaaa actgcgtcaa
     1081 ctggtggccg atagccgact ggtcagcggc agtgatcagc aggtcaccgt cagcattggc
     1141 gcggcgctaa ctcgccaaat tgagggcttt gagaccgtat ttaaacgcgc agatgcggct
     1201 ttgtaccgcg ccaaacgcca acgcaataca gttgaatatg aggcctgagg tggattttcg
     1261 taagtggtac cgcaggacta tggcgctagg gctgatcgtg agcctagtgg tgtttttagg
     1321 gcattttttt atccccacca aacgcttggc attagtaccg gaggggcact acgccatcaa
     1381 actttatggc ttcccagatg aacaattagg catttccgcc agctggctcg ataaagaaca
     1441 gaatcattgg ctgtgcaatt acaaaccctc ccacggctat gggtgcggct gggatgtcac
     1501 catcagtacc acacccggca aaggcatgga ttggacccag tacaacaagc tggaactgac
     1561 tatgcgctat aaagggcccg ccacgcgcct gcgggttttc ttacgtaatt ttaaccctgc
     1621 ctacgccaac gccgacgact tcgagtcgtt taaaaccatg aacatgtcct ttcccgtaga
     1681 ggatgtgagc aaacccatac tggttgattt acgggaattt caagtggtgg cctggtggtt
     1741 tgcgggcaaa cgcgcgcgcc tgg
//
LOCUS       DAOQGS010000761         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48572_length_1758_cov_4.604815, whole genome shotgun sequence.
ACCESSION   DAOQGS010000761 DAOQGS010000000
VERSION     DAOQGS010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48572_length_1758_cov_4.604815"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PKZ52_18590"
     CDS             complement(<1..899)
                     /locus_tag="PKZ52_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNG61836.1"
                     /translation="MNVLETLLVVYSLLLVLNGAVAIVLWLVYRKPILISLIGTWAFT
                     LFNFASQAATLNTELGSILGFGTYIFSSWCLCRIIATVLHGDFSFRPFWLGWGGSLIL
                     LAAAHLLQLDFWIKSLPIACAVAAPQLYWAYRALRGPADKSSALIKLFAAVLLINGLH
                     FIDYPFLRPIPEMAVVGYSVVIATTMLFGILLPCIINNFLAGLLANKLRNEIESHKAT
                     ELELESALIAAERSAKAKTIFLANMSHEIRTPINGILGLNDLLLESPLNEHQRAYANQ
                     VRKSSDELLNLVNNVLTLSKLESS"
BASE COUNT          456 a          405 c          434 g          463 t
ORIGIN      
        1 gaggattcaa gtttcgagag cgtcaataca ttgttcacca aattcagcag ttcatcactg
       61 gatttgcgca cttggttggc gtaggcccgc tgatgttcgt ttaggggaga ttctaacagt
      121 aggtcattca agcctaaaat gccattaata ggtgtgcgaa tctcatggct catattggct
      181 aaaaaaatcg ttttagcctt ggctgaacgc tctgcggcaa tcagcgctga ttctaattcc
      241 agctctgtgg ccttgtggct ttcgatttca ttgcgcagtt tattcgccag taaaccggca
      301 agaaagttat taataataca gggcagcaag attccaaata acatggtggt ggctatcact
      361 acgctgtagc ccaccacagc catttcggga attggtcgta agaaggggta atcgataaaa
      421 tgtagcccgt tgataagcag tacagccgca aacagtttga ttaatgccga cgatttatct
      481 gctgggccgc gcagcgctcg gtaggcccaa taaagctgag gggccgcaac ggcacaggca
      541 atgggtaggg atttgatcca gaagtccagc tgcaataggt gagctgcggc cagtaaaatt
      601 aaactccccc cccaaccgag ccaaaagggc cggaagctga agtctccgtg taatacagtt
      661 gctatgatgc ggcataggca ccagctgcta aaaatatagg tgccaaagcc caggatacta
      721 cccagctcag tatttaatgt ggccgcttga ctggcaaaat tgaatagcgt gaaggcccaa
      781 gtgccgatta aagaaatgag gatgggtttg cgatagacga gccacaaaac gatcgcaacc
      841 gcgccattta agaccagcaa aaggctgtaa acgaccaaca aagtttcaag aacgttcacg
      901 ttcggccctc aataatcggc tttggctgag tggtcagccc cattgcaagc ggggggtgta
      961 gcttcattgt tggttaaagg atggtaaggg ctctgcttta catgtgtaat ggcatcgcca
     1021 ggctgaatta taagctagat taaatttcac tgcttttaca ttgcatcttc tttggagcgc
     1081 catcagcgca atttcttcgg agcgccataa gcgcaatttt acttagcgcc ataagccaat
     1141 ttaacatagc gccgctgttt ataggcaact tgcctgtgac tcaaacaagg ctcgcgctgt
     1201 taattagccc acatgattcg caggctaaag aatctgtgcc tatgagtatt ttcggaaagt
     1261 gtgcacatac atgctatggc tctttcgcaa tgcgttctgc tttgaacgcc gtgaaaatac
     1321 ttgtaaatca gcgattacct cgccgccaat gcagtcgtcc ggctttttcg tcaaatttgg
     1381 ctgccagatc gtcgccgcct acgtatttca acaagctgct aggttgttgc aatattgacg
     1441 ttatgtcacg gaactattca catctgcgtt gcgcttcatc acagcggcaa tttactggca
     1501 cagtaggaat ctctggttag gcggcgtttt caaaggagcg cgtcaccttt tgcggctagg
     1561 ccacaggctg cttctatatc gtcaaaaaca ctgccagtca gaatctctgt agcacagggc
     1621 agcggacttt ctggctgccg tttatggcca agcgcattct cacaactcat cgtttgagta
     1681 taaaccagat tattgatagc ggaatgggcg gagatttttt cgtttgagta tttgttgcgg
     1741 gtcaattatt gttcccgg
//
LOCUS       DAOQGS010000762         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48661_length_1756_cov_4.628454, whole genome shotgun sequence.
ACCESSION   DAOQGS010000762 DAOQGS010000000
VERSION     DAOQGS010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48661_length_1756_cov_4.628454"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..467)
                     /locus_tag="PKZ52_18595"
     CDS             complement(138..467)
                     /locus_tag="PKZ52_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNG61837.1"
                     /translation="MAPSGPELIAVEVAYALPHEQRLLVVEVPPGTNALGAVEAARLA
                     DYFPEFVLDPTLLGLWGQAFGTKGLPKAADYQVKAGDRIECYRPLLCDPKEVRRLRAA
                     KAAVKRV"
     gene            complement(457..897)
                     /locus_tag="PKZ52_18600"
     CDS             complement(457..897)
                     /locus_tag="PKZ52_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNG61838.1"
                     /translation="MSVKQIQRSALVTFSAERMFALVNDIAAYPQFMEGCVSAVVLER
                     SEQEVLARLELQKMGIKQAFTTRNSLSPPQSMQMTLVDGPFKVFTGKWEFTSLGVEAC
                     KVSFELNYEFANSLLAMMAGKWMEAVANEQVEAICRRAKQVYGS"
     gene            1034..1510
                     /gene="smpB"
                     /locus_tag="PKZ52_18605"
     CDS             1034..1510
                     /gene="smpB"
                     /locus_tag="PKZ52_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNG61839.1"
                     /translation="MAKKKAPSDNTIAQNRRARHEFFIDERLEAGVALAGWEVKSLRA
                     GKGQLTDSYVVFHNGEAYLHNVLIQPLQTASTHFVTEPQRTRKLLLKAREIQRLREAN
                     EQKGYTILVLSLYWKAHLVKVEIAIAKGKQMHDKRDAEKDRDWARDKERTLREHNK"
     gene            1565..>1756
                     /gene="ssrA"
                     /locus_tag="PKZ52_18610"
     tmRNA           1565..>1756
                     /gene="ssrA"
                     /locus_tag="PKZ52_18610"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          461 a          522 c          428 g          345 t
ORIGIN      
        1 gggtggttgc cactccccgc caaggcatat ttgattagca ctacgctcaa tgctatttgg
       61 cggctattgc aggggtgttc gactgttaaa tccgccccat ggtaagtagc tcaactccca
      121 ctcaaaccca aacaacctca gacccgctta accgccgcct tggccgccct aagtcttctc
      181 acttctttag ggtcgcacag caaaggccga tagcattcga tcctatcccc cgctttcacc
      241 tggtaatccg ccgccttagg caaacccttg gtgccaaatg cttgccccca caaacccaaa
      301 agagtgggat ccaagacaaa ctcagggaaa taatccgcca ggcgcgcagc ctctacagca
      361 cccaaggcat tggtgcccgg cggaacctcc accactagca gtcgctgttc atggggcagg
      421 gcataggcca cctccaccgc gatgagctca gggccgctag gagccataaa cctgcttagc
      481 tctacggcag atagcctcaa cctgctcatt ggccactgct tccatccact tgcccgccat
      541 catggcaagc aggctattgg caaattcata atttaattcg aagctcacct tgcaggcctc
      601 aacgcccaga gaggtaaatt cccatttgcc ggtaaacacc ttaaacggcc catccaccag
      661 ggtcatctgc atggactggg gcggcgagag ggaattgcgg gtggtaaaag cctgtttaat
      721 gcccattttt tgcagttcca ggcgggctaa tacttcctgc tcactgcgct caagcaccac
      781 cgcgctcaca catccctcca taaattgggg gtaggccgca atatcattga ccagcgcaaa
      841 catgcgctcg gcgctaaacg tcaccaaagc actgcgctgg atttgcttca cactcattaa
      901 cctaaaaacc tctattgatg gcagcgagcc cgctaaaagg ctgcgaattg tactgcatag
      961 ggcagggaag gtatgggcca tctgcaaata aacgcggtaa gattgcgccc cctcacagaa
     1021 agacccaacc ggcatggcca agaaaaaagc acccagcgac aacaccatcg cgcagaaccg
     1081 cagagctcgc cacgagtttt tcatcgatga gcgcctggaa gcgggcgtgg ccctcgccgg
     1141 ttgggaagtt aaaagcttgc gagccggaaa aggtcagctc accgacagct acgtagtctt
     1201 ccacaacggc gaggcctacc tacacaacgt gctcattcaa cccttgcaaa ctgcctccac
     1261 ccattttgtt accgaacccc agcgcacccg taaattgctg ctaaaagccc gggaaattca
     1321 gcgcctccgt gaagccaatg agcaaaaggg gtatacgatt ttagtgctct ccctgtattg
     1381 gaaagcacac ttggtaaaag tggaaattgc tatcgccaag ggtaaacaaa tgcacgacaa
     1441 gcgcgacgcc gaaaaagacc gcgactgggc tcgcgataaa gagcgcaccc tgcgcgaaca
     1501 caacaaataa tccaatccgc agagtaattt aggtatactc gtcggccggg tgcaaactcg
     1561 gattgggggc gattaggatt cgacgccggt agtgaagccc aaggtgcatg tggtgatggc
     1621 agcgaatcac cttaatccaa agctgcaact gaatagtcgc aaacgacgat tcatacgctc
     1681 tagcagctta ggctgctagc ggtcagcgac aaggtgccag taccgcgtaa ctgactgtca
     1741 gatagaactg gatcgc
//
LOCUS       DAOQGS010000763         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48912_length_1751_cov_3.361439, whole genome shotgun sequence.
ACCESSION   DAOQGS010000763 DAOQGS010000000
VERSION     DAOQGS010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48912_length_1751_cov_3.361439"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKZ52_18615"
     CDS             complement(<1..612)
                     /locus_tag="PKZ52_18615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNG61840.1"
                     /translation="MAFAKTFLLSFLAPVSVIAPAALNAAESAATHSEFGEVVVTASR
                     ISQPLSQVGVSVDVLNRDDLMQRNSASLADILRTIPGINVSNSGGLGKATSVFVRGED
                     AFRTRLYIDGIAVSDTTATQISPRFDALLNQQLGRVEVLKGPQALIYGADSGGVISVF
                     TPEASDAVNADVDAETGSFNSRNISANVRGKNETFDYFISSGAL"
     regulatory      complement(745..954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1041..>1751)
                     /locus_tag="PKZ52_18620"
     CDS             complement(1041..>1751)
                     /locus_tag="PKZ52_18620"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNG61841.1"
                     /translation="NFSQPRAQAVSSKIHDIRFLLHRRNPSMPLDSLLQFILITGAVS
                     ISPGPVMLLCLALGSRLPLARVSAAMLGASLGNCVLMVLSAAGLGSLVALWPKAFGAI
                     ALAGGLYLFYLGVMLWRAPWVDLSSAAQPQLNSYGALLQRGFWVAASNPKGIVYFGAL
                     IPPFLQHSHHYTLTAIMLTGIFLAIDLAVMLAYAKLGRQLTQLLKLPSTQRRCNQILA
                     SFLMLMAVWLCADTIHTF"
BASE COUNT          472 a          435 c          462 g          382 t
ORIGIN      
        1 gagggcgcct gaactgataa aataatcaaa ggtttcattt ttaccgcgca cattggcgct
       61 gatgttgcgg ctgttaaaac tgcccgtttc ggcgtccaca tcggcattaa cggcatcgct
      121 ggcttccggt gtaaatacgg aaattacccc gccggagtcg gcgccataaa taagcgcctg
      181 agggcctttt aacacttcta cgcggccgag ttgttgattt aataatgcat caaaacgcgg
      241 tgatatttgc gtggcagtgg tatcgctcac tgcaatacca tctatatata aacgggtgcg
      301 aaaggcatct tcaccgcgca caaatacact agtggctttg ccaaggccgc cggaattgct
      361 cacattaata ccggggatag tgcgcaaaat atccgctaaa gatgcgctgt tgcgctgcat
      421 taaatcatcg cgatttaata catccaccga tacgcccacc tgtgataggg gttggctaat
      481 gcggctggcg gttactacca cttcgccaaa ttcactgtga gttgcggcgg attctgccgc
      541 gttgagtgct gccggggcaa taacacttac cggcgcaaga aaggataata aaaatgtttt
      601 ggcgaacgcc ataaaaccag tctcccaaac agaacatcgt tgggaaaaca aacggccggt
      661 cgcggcgcaa gccttttggc gcgccgcgca ataccacgcc acctgccctc cgcaagttgg
      721 taaatatcta accgacgatt gccggttaga ggtcacttcg aaggccggta tccgggctta
      781 tggccgcttg ataacaagcg gggcaacgcc ttcccatgtc aaaacacagt ggcggatgtt
      841 gccttgcacc taaaaaatag gggtaaccaa tgaccgttgc gggggcagcg caggattttc
      901 acctgcttcc cgtttaacta aacagggttt agcacctagg aagggcggcc atactaggga
      961 caagtctttg caaaggcaag cgcggtattg ttcgcttgca gcggtggtcg cccatggctc
     1021 gagcctgttt taacagattg ttaaaaagta tgaattgtat cggcacacaa ccacactgcc
     1081 atcagcatta aaaaactcgc caatatttga ttgcagcggc gttgggtgct gggtaatttt
     1141 aaaagctggg ttaattgtcg gcccagtttg gcataggcca gcataacggc taaatcaatg
     1201 gccaaaaaaa tacccgtgag cataattgcc gtgagcgtgt agtggtggct gtgttgtaaa
     1261 aaaggcggaa ttaacgcgcc aaaataaaca atacctttgg ggttgctagc cgccacccaa
     1321 aatcctcgct gcaataaagc gccgtaacta tttagctgcg gctgggcggc agaggataaa
     1381 tccacccagg gtgcgcgcca aagcattaca cctaaataaa ataaatacag cccgcccgcc
     1441 agtgcgatag cgccaaaggc ttttggccac agcgccacta aactgcctag cccagcggcg
     1501 gataaaacca ttaatacaca attgccaagg ctggcaccca gcatggcggc gcttacccgt
     1561 gcaagcggta aacggctgcc cagggccagg cataacagca ttactgggcc ggggctgatg
     1621 ctgacggcgc cggtgattaa aataaattgc agcagtgaat caaggggcat tgaggggttc
     1681 cggcgatgca gtaaaaagcg aatatcgtgt attttagagg aaacagcctg agcgcggggt
     1741 tgagaaaaat t
//
LOCUS       DAOQGS010000764         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_48988_length_1749_cov_4.309327, whole genome shotgun sequence.
ACCESSION   DAOQGS010000764 DAOQGS010000000
VERSION     DAOQGS010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48988_length_1749_cov_4.309327"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1749)
                     /locus_tag="PKZ52_18625"
     CDS             complement(<1..>1749)
                     /locus_tag="PKZ52_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61842.1"
                     /translation="LSTTRRFGGTGLGLAIARELATLLGGRIEVQSQPGQGDLSIAAG
                     ATFNAGAIDLTADQGAINLAGKLLTPAGDVGLWAKGDIHLAPTAAMSAQALSLASQTG
                     SIQADSGSQLIHGNSGKPGELILSASPDGLLASSLRANIEGWNLTAEIWLREEATDGK
                     VSLAQLEAMNTQTESFLAAHPDLFTALTPGQTAAGGVSLHAELYSSGDIEVAEKLDLS
                     NWRLGKTPVPTTGEEPGLEEPGLEGEAPAENNEELLADSGLSLEGQEPPALEAPSETL
                     PASATPANPGRAGRLTLRSGGNIQLNGSISDGFAADTSSWGSQFVDVNGMPLDALMSE
                     DSSHINLISGADLTSSNVLNTQARQGDAGSLTLADEVTVRTGTGAITIATQGNLQMAQ
                     GGTATIYTAGKTQMRKYAGSDELLPDYGTVHPYTVNEYALGSLFINKVYYPEAGGDIR
                     IRTGGDIQGPQSHQLVNEWLHRAAGTFVQTQDPVNNPTDETNAVTRKITTWGIGFQDF
                     NQGIASLGGGNLNIHSGGNIDDLAVSSANTGKAAGEGLNNQVQQSLSGAVSIQAAGDI
                     NSGRWYSANNRFEVAS"
BASE COUNT          291 a          506 c          545 g          407 t
ORIGIN      
        1 ggcttgccac ctcaaagcgg ttattagctg aataccagcg gccggagtta atgtcccccg
       61 ccgcctggat ggaaacggca ccactgaggc tctgctgcac ctggttatta agtccctcgc
      121 ctgccgcttt acctgtattg gccgaggaca cggctaaatc gtcgatatta ccgccgctgt
      181 gaatattcag gttgccgccg ccgaggcttg cgatgccttg gttgaagtcc tgaaagccta
      241 tgccccaggt ggtaattttg cgggttacgg cattggtttc atcggtgggg ttgttgactg
      301 ggtcttgggt ctgcacgaag gtgcctgccg ctcggtgcag ccattcattc accagttgat
      361 ggctttgcgg gccttggatg tcgccgccgg tgcggatgcg gatatcgccg cccgcctctg
      421 gataatacac cttgttgata aacaagctgc ccaatgcgta ttcattgact gtgtaggggt
      481 gcactgtgcc atagtccggc agaagttcat cactacccgc atacttgcgc atctgggttt
      541 tgcccgccgt gtagatggtg gcggtgccgc cctgagccat ctgtaaattc ccttgggtgg
      601 cgatggtgat agcgccggtt ccggtgcgaa cggtcacctc atcggcaagc gtcaggctac
      661 cggcatcgcc ctgccgggcc tgggtgttta atacattgct actggtaagg tccgcgccgc
      721 tgataaggtt gatatggctg gagtcctcgc tcatgagggc atctaaaggc ataccgttaa
      781 catccacaaa ttggctgccc cagctgctgg tgtcggcggc aaagccatca ctgatggagc
      841 catttaactg aatattgcca ccggagcgca gggtaaggcg cccggcgcgg cctgggttgg
      901 caggtgtggc actggccggg agggtttcgc ttggcgcctc cagtgcaggc ggctcctgac
      961 cttccaggct gaggccgctg tcggccaata attcttcatt gttctctgct ggcgcttctc
     1021 cctcaaggcc cggctcttcc aaacctggct cctcacctgt tgtaggcaca ggggttttgc
     1081 ccaggcgcca gttgcttagg tccagttttt cggcaacttc aatatcgcca ctgctgtaca
     1141 gctccgcatg taggctcaca cccccagcgg cggtttggcc gggggttagg gcggtgaaca
     1201 ggtctgggtg ggcggctaaa aagctttcgg tttgagtatt catagcctcc aactgggcca
     1261 ggctcacctt gccgtcggtc gcttcttctc gcagccaaat ttctgcagtg aggttccagc
     1321 cctcgatatt agcccgcagg cttgaggcca agaggccgtc ggggcttgca ctcaggatta
     1381 gctcgccggg tttgccgcta ttgccgtgaa ttaactggct gccgctatcg gcctgaatag
     1441 agccggtttg gctggcaagg cttagggcct gggcactcat ggcggcagtg ggggcaaggt
     1501 ggatatcgcc cttggcccac aggcccacat cccctgcggg ggtgagtagt ttgcccgcga
     1561 ggttaatagc accttggtcg gcggtaaggt ctatggcccc ggcgttaaag gttgccccgg
     1621 cggcaatgga taggtccccc tggccgggct ggctctgcac ctcaatgcgg ccgcccagca
     1681 gcgtggccag ctctcgcgcg atcgccaggc cgagcccggt gccgccgaag cgccgcgtgg
     1741 tcgacaggt
//
LOCUS       DAOQGS010000765         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_49523_length_1738_cov_3.938206, whole genome shotgun sequence.
ACCESSION   DAOQGS010000765 DAOQGS010000000
VERSION     DAOQGS010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49523_length_1738_cov_3.938206"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKZ52_18630"
     CDS             <1..764
                     /locus_tag="PKZ52_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61843.1"
                     /translation="FYYIAGDNSTDPTSANFNQYGELKQRYLDYQEGKVGSDSVLDIG
                     EGINEYINQMAAAPAAMLPPTNVFRIVPPGTRSLFVTAFKDLDELYQSLKISPRMEKI
                     EVGLSHWKPGSIYNDGKLGETLMQDVLEKLTGKRFTAIQNTSGHGPDGIAIDHTKTPV
                     EIYVAEAKSSVNGANAAERPSGDPNARLSGWVDKYLTGKFDTASPEGRAMFDQIINTC
                     GVDLIRCNVRGIWAQVEVPRPGATKVSLDATLKAW"
     gene            769..>1738
                     /locus_tag="PKZ52_18635"
     CDS             769..>1738
                     /locus_tag="PKZ52_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61844.1"
                     /translation="MDSLYQVKAPKFRSRKAFIAHAARVHEMKSSGPLFEGDKMRLAK
                     FMTAERFSSQTFTDAVLTWRSNLLHDYLMQSAAVLNEHGGSINQLRETVFSHGINLQI
                     YFMSWELQRVEIEELFPHKTYNPLVGPNIRDVGDYICLISIFSNIFYFSQMVKTILDH
                     IDLGHLVISHYANVTQQFILRLAEDFLQLPRRNWEADVGNWAFDPLEKEPLFKAIWDH
                     WADEDTANLEPMLIQLLNWHTYQASRENKDGGQDYYPYVEQIPLAYFFILRLRQWRGL
                     SVPKISHVLTQPPFDSLPDEVEGRYEFCLFAKEFYAKTISLMPDFID"
BASE COUNT          505 a          379 c          392 g          462 t
ORIGIN      
        1 gcttttatta catcgcaggt gacaattcaa ccgatccaac aagcgccaac ttcaaccaat
       61 acggcgaatt aaaacagcgt tatctggatt accaggaagg taaagtaggt tccgatagtg
      121 tcttagatat tggcgagggc ataaacgagt acatcaacca gatggcagcg gctcctgcag
      181 ccatgctgcc accgacgaat gtattccgca ttgttccacc aggcacgcga tcattattcg
      241 taacagcatt caaggatctt gatgaactct atcaaagctt gaaaataagc ccacgaatgg
      301 aaaaaattga ggttggctta agccattgga aaccgggttc tatctataac gatggaaaat
      361 taggcgaaac cttaatgcag gatgtactag aaaaattaac gggcaaacgc tttaccgcaa
      421 ttcaaaacac cagcggccat ggcccggatg gaattgctat tgatcatact aagacacccg
      481 ttgaaatcta tgtagcggag gcgaagtctt cggttaacgg ggctaatgcg gccgagcggc
      541 caagcggtga cccaaatgca cgactaagcg gatgggtaga taaatactta accggtaaat
      601 ttgatacagc gtcgccagaa gggcgcgcaa tgtttgatca aattataaat acatgtggtg
      661 tagacctaat tcgttgtaac gtacggggca tatgggcgca ggtcgaagtc cctcgccccg
      721 gtgcgaccaa ggtgtcccta gatgcaacac taaaggcctg gtaacaacat ggactcactt
      781 tatcaagtca aggcgcccaa gtttcgctca cgcaaagctt ttatcgctca tgcagcacgt
      841 gttcacgaga tgaaatcgag tggcccactg tttgaaggtg ataaaatgcg acttgcaaaa
      901 tttatgactg cagaaagatt ttctagtcag acctttacag atgcggtttt gacttggaga
      961 tcaaatttgc ttcatgacta cctcatgcaa tctgccgcag tactaaatga acatggaggt
     1021 agcatcaatc aattgcgtga gactgtcttt agccatggaa ttaaccttca aatctatttt
     1081 atgagctggg agttacaaag agttgagatt gaagaactat ttccacataa aacttacaat
     1141 ccgctggtcg ggcccaatat ccgtgatgtt ggtgattaca tatgtctaat ttcaatattt
     1201 agtaatattt tttatttttc acagatggta aaaactattt tggaccacat agatttaggc
     1261 cacctggtaa ttagtcacta cgccaatgtg acacaacaat ttattctgcg cctagcggag
     1321 gattttttac agctgccccg ccgcaattgg gaagctgatg tcggcaattg ggcctttgac
     1381 cctcttgaaa aagagccttt atttaaagca atttgggatc attgggccga tgaagatacg
     1441 gcgaatttag agcccatgtt aatacaacta cttaattggc acacctatca ggccagccgt
     1501 gaaaataaag acggcgggca ggattattat ccatatgtag agcaaattcc cctggcctat
     1561 ttttttattt tgcgtttgcg ccagtggcga ggtttaagtg tgcctaagat ctcccatgtg
     1621 cttacccagc cgccttttga ttcacttcct gatgaagtgg aaggccgcta tgagttttgc
     1681 cttttcgcta aagaatttta cgccaaaacc atcagcctga tgcctgactt catagatc
//
LOCUS       DAOQGS010000766         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_49574_length_1737_cov_4.452438, whole genome shotgun sequence.
ACCESSION   DAOQGS010000766 DAOQGS010000000
VERSION     DAOQGS010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49574_length_1737_cov_4.452438"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PKZ52_18640"
     CDS             complement(<1..1015)
                     /locus_tag="PKZ52_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61845.1"
                     /translation="MRLIKWTILLGIVAGVFWAAVPWSLRWYLNQQLTQLGLESRLRD
                     LRINYVAGEFILQGWEVRRQGELVLRVEQLRLSPDLAAWREDRLVLRKLAAQSLRLNW
                     QSADKGITFAGQPAAQWQQLLKLERGAQLNSVTLENAELCQSGGSLDQCWVIGTGQGS
                     DLVWRWDRAGWQLASRSSLVLSKTHLQNQQVNLAVFLLETSQMRDFVFSNDWSRIGQW
                     QLNGVHLVERSLDEQKNVENAYQTQAASVLINDLGIKTGQPTQLHIGQLDITRLRQTL
                     HQNREQVMLATAQLRRFLPFTEQFFNPAAPLTLAIDKTHIVDSAVAWRDDSVTPAVTE
                     SLVG"
     gene            complement(1267..>1737)
                     /locus_tag="PKZ52_18645"
     CDS             complement(1267..>1737)
                     /locus_tag="PKZ52_18645"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG61846.1"
                     /translation="QAPFLQLPAGAELDLGGIGKEYAADKALLMLTQATYGQEAAFLV
                     NFGGDIAASGPRPSASHWQVGLAQGDKVLQIPLLAGGIATSGDSHRYIWHQGVKLSHI
                     LNPLTGWPVTGGPSTATVFAQNCTQAGMLATFALLHGPAAEDFLNQQNCTYILT"
BASE COUNT          342 a          494 c          515 g          386 t
ORIGIN      
        1 gccccactag ggattcggta acggcggggg ttacgctgtc atcccgccag gccacggcgc
       61 tatccacaat atgggttttg tcgatggcca gggtcagggg ggccgctggg ttaaaaaact
      121 gttcggtaaa gggcaggaat ctgcgcagct gagcggtggc cagcatgacc tgttcgcggt
      181 tctggtgcag ggtttggcgc agccgggtaa tgtccagttg gcctatgtgc agctgggtgg
      241 gctggccggt tttaatgccg agatcattaa tcagcaccga ggccgcttgg gtctggtagg
      301 cattttcgac atttttttgc tcatcgaggc tgcgctccac taggtgcacc ccatttaact
      361 gccactggcc gatacgggac cagtcgttac tgaagacaaa atcccgcatc tgggaggttt
      421 ccagcaaaaa caccgctaaa ttgacttgct gattttgcag gtgggttttg cttaacacca
      481 ggcttgagcg gctggccagc tgccagcccg cccggtccca gcgccagact aaatcgctgc
      541 cttggcccgt gcctatcacc cagcactgat ccaggctgcc gccgctctga cacagttcgg
      601 cgttctctag ggtgaccgaa tttaattgcg ccccgcgctc taatttaagc agctgttgcc
      661 attgggcggc gggttgccct gcgaaggtga tgcccttatc ggcgctctgc cagttcaggc
      721 gcaggctttg ggcggcgagt ttgcgcagca ctaaacggtc ctctcgccaa gcggccaggt
      781 ccgggctcaa gcgcagttgc tccacccgca gcaccagttc accctgcctg cgcacttccc
      841 agccctgcag aataaattcc ccggccacat agttgatcct taagtcgcgc aggcggcttt
      901 caaggcccag ctgggtcagc tgctgattga gataccagcg caggctccag gggactgccg
      961 cccaaaaaac gccggccact atgcctaaca ggatggtcca tttaatcaac cgcatgctgt
     1021 gtcgtcctca ggtatttatt cttgttctgc gcagagttgc gaacagccct gcctatacat
     1081 cgttaatggg tttggcctgg aacataccac agccgaattc ttcaccgcaa aagcgtcatt
     1141 taacctaaaa accgcagtag ggttttaggt taagcctagc cctgtatcgg ccagcctgca
     1201 gcggcaatac caatggcaag aatagccttt gccagcaagg tgtgggcagt cgcagctggt
     1261 gcagccttag gtgagtatat aggtgcagtt ttgttgattt aaaaagtctt cggcagcagg
     1321 gccgtgcagt agcgcaaaag tggccaacat gcctgcctgg gtgcagtttt gcgcgaacac
     1381 ggtggcggtg gaggggccgc cggtgaccgg ccagccggtg agggggttga gaatgtggct
     1441 gagttttacg ccctgatgcc aaatgtagcg gtggctgtcg ccgctggtgg ctatgccgcc
     1501 cgccagtaaa ggaatctgca gcactttatc accctgcgcc aggcccacct gccaatggct
     1561 agcgctgggg cgggggccgc tggcggcaat gtcgccgccg aaattcacca aaaacgccgc
     1621 ttcctgccca taggttgcct gggtgagcat cagcagggct ttgtcagctg cgtattcttt
     1681 gcctatgccg cccaaatcaa gttctgcgcc cgcaggcagt tgtaagaaag gcgcctg
//
LOCUS       DAOQGS010000767         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_49637_length_1736_cov_3.779298, whole genome shotgun sequence.
ACCESSION   DAOQGS010000767 DAOQGS010000000
VERSION     DAOQGS010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49637_length_1736_cov_3.779298"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PKZ52_18650"
     CDS             complement(<1..1103)
                     /locus_tag="PKZ52_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNG61847.1"
                     /translation="MHNQSIASQLAQLRSRSISSRELTQHYLERIARLDSTYNSFVTV
                     TAEQALAQADAADKLLAQGNAHPLCGLPIAHKDIFCTQGVRTSCGSKMLDNFIPPYNA
                     TIVENYLNAGVVMLGKTNMDEFAMGSSNETSYYGPVKNPWNTSCVPGGSSGGSAAAVA
                     ARLAPAATASDTGGSIRQPASLCGISGIKPTYGRISRWGMIAFASSLDQAGLMATSAE
                     DLALLLNVLTSFDNKDATCIDHPIPDYAASLSQPLKGLKIGVPVEYFGAGLHPQTEAA
                     VKAALAQFEAMGATLVEITLPHSALSVPAYYVIAPAECSANLSRFDGVRYGHRCKDPK
                     DLLDLYKRTRSEGFGPEVQRRILIGSYVLSHGY"
     gene            complement(1166..1447)
                     /gene="gatC"
                     /locus_tag="PKZ52_18655"
     CDS             complement(1166..1447)
                     /gene="gatC"
                     /locus_tag="PKZ52_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNG61848.1"
                     /translation="MDADQIEKLAKLARLNIAPDQAGEVAERISNVLKMVDQLQALDT
                     TGVAPMSHPLDAVSRLRADEVSEPNVREAFMAIAPACEDGLYLVPKVIE"
BASE COUNT          345 a          436 c          567 g          388 t
ORIGIN      
        1 tagtaaccgt ggctcagcac atagctgccg atcaggatgc ggcgctgcac ctcggggccg
       61 aagccctcgc tgcgggtgcg tttgtagagg tccagcaggt ctttcgggtc tttgcagcgg
      121 tggccgtagc gcacgccgtc aaaacgcgac aggttagccg aacactccgc cggggcaatc
      181 acgtaataag ccggcaccga tagggccgag tggggcaggg tgatttctac cagggttgcc
      241 cccatggcct cgaactgggc cagggcggct ttaactgccg cttcggtttg cgggtgtaaa
      301 ccggcgccga agtactccac cggcacacca attttcaggc ctttaagcgg ttggcttaaa
      361 gaggctgcat agtctgggat ggggtggtcg atacaggtgg cgtctttatt atcgaagctg
      421 gtgagcacat tgagcagcag ggctaagtcc tctgccgagg tggccatgag gccagcttgg
      481 tcgaggctgg aggcaaaggc gatcatgccc cagcgcgaga tgcgcccata ggtgggttta
      541 ataccgctga taccgcagag gctcgccggt tggcgaatcg agccgccggt gtccgatgcg
      601 gtagccgccg gtgctaaacg cgcggctacc gccgcagccg agccgcctga ggagccgccg
      661 ggtacacagc tggtattcca ggggtttttc accgggccgt agtagctggt ttcattggag
      721 gagcccatgg cgaactcatc catattggtt ttgcccagca tcaccacccc agcattgagg
      781 tagttttcca cgatggtggc gttgtaaggc gggatgaagt tatccagcat tttgctgccg
      841 cagctggtgc gcacgccctg ggtgcagaag atatctttgt gggcgatggg caggccgcac
      901 agggggtggg cattgccctg ggccagcagc ttgtcggcgg cgtccgcttg cgcaagggcc
      961 tgctcggcgg tgacggtgac aaaactgttg taggtgctgt ccaggcgcgc tatgcgctct
     1021 aggtagtgtt gggtcagctc gcgactggag atactgcgac tgcgcagttg ggccagttgg
     1081 ctggcgatgc tctggttatg catggggcct ggggctcctg taaaagaggg cgaaaattaa
     1141 aatgcagatc gacgggccga tacccttatt caatgacttt gggtaccagg tacaggccgt
     1201 cctcgcaggc gggggctatg gccataaagg cttcgcgcac attgggttcg ctcacctcat
     1261 ccgcgcgcag gcgggagacg gcatccagcg ggtgggacat gggcgcaacg cctgtggtgt
     1321 ccagcgcctg cagttggtcc accattttta ggacattgct aatgcgctcg gccacttccc
     1381 ctgcttggtc gggggctatg ttgaggcggg cgagtttggc gagtttttca atctggtcgg
     1441 cgtccacgaa gggtctcccg tgaaaataag tgagcttgag ttaagtaaac aacattaggt
     1501 tctgggtagg gcggtgaggc gaaggtagca ataatttggt tggttaaaga aaaagcaaat
     1561 tcaggtataa aactattgag aaatttgatc ggatggtgat gattcctgga catgtttttt
     1621 ggtgtcatca catcggcata ttttaattct tcacccaaag gcctgagatg ctttccaccg
     1681 aagcaaactc attacaggcg ggggtggaaa gtccatatac agtcaccgtt ttgcca
//
LOCUS       DAOQGS010000768         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_49793_length_1733_cov_6.566150, whole genome shotgun sequence.
ACCESSION   DAOQGS010000768 DAOQGS010000000
VERSION     DAOQGS010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49793_length_1733_cov_6.566150"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            660..>1733
                     /locus_tag="PKZ52_18660"
     CDS             660..>1733
                     /locus_tag="PKZ52_18660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNG61849.1"
                     /translation="MRLLLIDDDELDRLAVIRALKKSTTPHEICQCANAADGLRMSAA
                     ENFDVILLDYRLPDMNGLAVLRQLRSGNFAEVAVVMLSSIEDDAIAEQCFEAGAQDFM
                     LKDEVNGRRLIRAVRQAKQRYEIERALQNSHDQLRILAERDPLTGLSNRRGFDLALKA
                     AIAHAKRTHESIALVLIDLDDFKTVNDTLGHEAGDKLLVELSVRLREMVRDSDFICRL
                     GGDEFLILATSVQKEEQIALLVERLNNALHNPIKINGTDILITASIGVALLGQRADNA
                     DELFKCADIAIYRAKQNGRNQSHFYNEQLDALVKHKAKIKLDLYKALTHNEFKLYYQV
                     KINAADGSIGSAAHPVRLDLGDAA"
BASE COUNT          486 a          422 c          441 g          384 t
ORIGIN      
        1 cagggaggta ggaggtagag caatgcagga gcaattgccg agtttacacg cagtaaatga
       61 gcattttttt gccaaattca acgacgctag ggcgagtgag gtaatttttc agaggttccc
      121 taattttcgg ccagcatgga gagcgtgtaa taagatatgc tgaatagttg caaaccgtgc
      181 ctattctacg ggcacacagg cgcttaaaac gcgggctttc tcgcccgcgt ggaaaagata
      241 taaacccgcc tacagcgtgc aggtgattac agcctccgac atccctcaag gtttgcattt
      301 aagccactcg gcgaaaaccg ccctaaacca aaacgaaccc gtttacccgt tacagcgctg
      361 agcaaataac tctcaaccgc ttgattaatt gcaataaagc gcacatctgt gccggttagc
      421 aatacagaga taaaacacta tactgagtgg gtttggtggg caaagcgctg tgcctgtgcc
      481 cacaacaaaa ttagggagaa atgccgcagg agcttgaagt agccgaatag gtatccaaaa
      541 ccaattgggc accaacaacc acttgagcaa gacggaagca cccgtctaat tagcgcaaca
      601 cggcattggg gcggggcatt ggccgcccag aggttagtag gaatggaaat tatataatta
      661 tgcgtttatt gcttattgac gacgacgagc ttgatcgact agcggtaatc agagcgctaa
      721 aaaaatcgac cactccccat gaaatatgcc aatgcgcaaa cgccgctgat ggcctgcgca
      781 tgagcgctgc agaaaatttt gacgttattt tgctcgatta ccgcctgccc gatatgaacg
      841 gcctagctgt tctgcgccag ctgcgctcgg gtaattttgc cgaggttgcg gtggtgatgc
      901 tcagtagtat tgaagatgat gccatcgccg aacagtgttt tgaagcgggc gcgcaggatt
      961 ttatgctcaa ggacgaagtc aatggccgcc gcttaatccg cgccgtgcgg caggcaaaac
     1021 agcgctacga aatagaacga gccctacaaa atagccacga tcagctgcgc attctggccg
     1081 agcgggaccc gcttaccggc ctgtccaatc ggcggggttt tgatttagct ttaaaagcag
     1141 ccattgccca tgctaagcgc acccacgaaa gcatcgccct agtattaatc gacctggatg
     1201 attttaaaac cgtcaacgat accctcgggc acgaggcagg ggataaattg ctggtggagc
     1261 tatccgtgcg gctgcgggaa atggtgcgcg atagcgattt tatttgcagg ctcggtggcg
     1321 atgaattttt aattctggcc acctcagtgc aaaaagaaga acagattgca ttgttggtgg
     1381 aacgtttaaa caatgccctg cacaacccaa ttaaaattaa tggcacggac attttaatta
     1441 ccgctagcat cggcgttgcg ctactcgggc aacgggcaga taacgccgat gaattattta
     1501 aatgcgccga tatcgccata taccgtgcca aacaaaatgg ccgcaatcaa agccattttt
     1561 acaatgagca gttagatgcg ctagtcaaac acaaagccaa aattaaactg gatttataca
     1621 aggccctcac ccacaacgaa tttaaactct attaccaggt aaaaattaac gccgccgatg
     1681 gcagcatagg cagtgcggcg catccggttc ggctcgatct cggcgatgcg gcg
//
LOCUS       DAOQGS010000769         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50140_length_1727_cov_4.537679, whole genome shotgun sequence.
ACCESSION   DAOQGS010000769 DAOQGS010000000
VERSION     DAOQGS010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50140_length_1727_cov_4.537679"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKZ52_18665"
     CDS             complement(<1..281)
                     /locus_tag="PKZ52_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNG61850.1"
                     /translation="MFFDRPESGELAVLVHIDFNRDDEPNDPREFEELALSAGADPVA
                     FVTGVRDVPNAKFFVGTGKLAEIQAQVIEHGAQLVVFNHSLSPSQERNL"
     gene            complement(296..553)
                     /gene="hfq"
                     /locus_tag="PKZ52_18670"
     CDS             complement(296..553)
                     /gene="hfq"
                     /locus_tag="PKZ52_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNG61851.1"
                     /translation="MSKGHSLQDPYLNVLRKERIPVSIYLVNGIKLQGQVESFDQFVV
                     LLKNTVSQMVYKHAISTVVPSRPVRVPMVTDLSDDEEETGA"
     gene            complement(687..1649)
                     /gene="miaA"
                     /locus_tag="PKZ52_18675"
     CDS             complement(687..1649)
                     /gene="miaA"
                     /locus_tag="PKZ52_18675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNG61852.1"
                     /translation="MHPLPPVIALMGPTAAGKTDLALALAQQLNGEIISVDSALVYRG
                     MDLGTAKPSPYEQAQVPHHLIDLCDPAQPYSAADFCRDARAAIVDILTRGKQPILVGG
                     TMLYFKALLDGLSDMPATTQEVRAQVEAEAEAKGWPALHSELAEVDPITAERLHPNHS
                     ARIGRALEVYRMSGIPMSQWQSSNSGGLWQQYDWRQIAIAPKQRSLLHERIALRFDAM
                     LAGGLIDEVAGLRARPDLHLGLPSMRAVGYRQVWQYLAGECDFGQMRKAGIAATRQLA
                     KRQFTWLKGWHDLSWVYTQADDGSGVSSEEILIKTLQLLALPPI"
BASE COUNT          358 a          488 c          526 g          355 t
ORIGIN      
        1 tcgaggttgc gctcttggga gggggagaga ctgtggttaa acaccaccaa ctgggcgccg
       61 tgctctatta cctgcgcctg aatctcggcg agtttgccgg tgccgacgaa aaatttagca
      121 ttgggcacat cgcgcacccc ggtgacaaaa gccaccgggt ctgcgcctgc ggacagggct
      181 agctcttcaa attcgcgcgg atcattcggc tcgtcatcgc ggttaaagtc gatatggact
      241 agaaccgcta actcaccgga ttcgggtcta tcaaaaaaca agaattactc tcgatttagg
      301 cgccggtttc ttcctcgtca tcggacaggt cggtcaccat ggggacgcga acggggcgcg
      361 aggggactac ggtggaaatg gcgtgtttgt ataccatctg gcttacggtg tttttcagca
      421 gcaccacaaa ctgatcaaat gactccacct ggccttgcag cttgatgcca ttaaccaaat
      481 agatggacac ggggatacgc tctttgcgca gcacgttaag gtaagggtct tgtagactgt
      541 gcccttttga catggggggt ctccttgtag gaagaaggaa aaagttaagc ttaaacgggg
      601 ttggcccgtc gtcgccgaga atttaataat ttaaagcatc gctttagatg acagaaccgc
      661 ataagaacgg ttcacaagcg ccgccattat attggtggca aggctagaag ttgcaaggtt
      721 ttaatcaaaa tttcctcgct ggacacccca gagccgtcat ccgcttgagt atagacccag
      781 ctcaaatcgt gccatccttt cagccaggtg aattggcgtt ttgccaactg gcgcgtggcg
      841 gcgataccgg ccttgcgcat ttggccaaaa tcgcactccc cggccaggta ctgccacacc
      901 tgccgatagc ccaccgcccg catggagggc aggcccaggt gtaaatccgg ccgcgccctc
      961 agccctgcca cttcatcaat caatcccccc gccagcatgg cgtcaaaccg cagggctatg
     1021 cgttcgtgca acaggctgcg ctgctttggg gcaatagcga tctgccgcca gtcgtactgc
     1081 tgccacaggc caccgctgtt gctgctctgc cactggctca tggggatgcc ggacatgcgg
     1141 tacacctcca gagcgcggcc gatgcgggcg gagtggttgg ggtgcaggcg ctcggcggtg
     1201 ataggatcca cctcggcaag ctcgctatgc agggcgggcc atcctttggc ctcggcttcg
     1261 gcctctacct gggcgcgcac ctcttgggta gtagcgggca tatcggagag gccatccagc
     1321 agggctttga aatacaacat ggtgccgccc accaggatgg gctgcttgcc ccgtgtgagg
     1381 atatcgacaa tcgccgcgcg ggcatcgcga cagaaatcgg cggcgctgta aggttgcgcc
     1441 ggatcacaca ggtcgatgag gtgatggggc acctgggcct gctcatacgg cgagggcttg
     1501 gcggtgccca gatccatacc ccgatacacc agggcggaat ccacgctgat aatttcgcca
     1561 tttaactgct gggctagcgc cagggccagg tcggttttgc cggcggcggt ggggcccatc
     1621 aaagcgatga cgggaggcaa ggggtgcatc actggccccg caggaacatg gtgtccaact
     1681 ggcccaggct cagctgggtc caggtggggc ggccatggtt gcactgg
//
LOCUS       DAOQGS010000770         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50292_length_1724_cov_5.159377, whole genome shotgun sequence.
ACCESSION   DAOQGS010000770 DAOQGS010000000
VERSION     DAOQGS010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50292_length_1724_cov_5.159377"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..>1724)
                     /locus_tag="PKZ52_18680"
     CDS             complement(307..>1724)
                     /locus_tag="PKZ52_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61853.1"
                     /translation="VIRHDQAQFIYHLPALNPAELGLSGWGNFRLRVLLLAYGYDTQP
                     RFVLSNALGNEGDTRMSWKPIWLRHWPSVTAYIPGSTRGIMDPFELLYPVTPILNRAI
                     GFEVAFELHNLTTFRWVVQLTNRLDFTVGLIVQAAGWQAWITLAAGQTRYVSGATAVS
                     GMGNWNAYLLHPFSPLYGWHRNWSNEFMRMADGECQVLFRVYLTAPDQADCIPPELPP
                     LQAGLRWQFNPTDLEMRFIPRWSAIKRFSRTGVYEYDFNTADDPILERTPATRLPAYS
                     MLDPATYFAQGYDTAPVDTSPWHKRPWSQAQQMPIMCRGEKLSAEGFETLWVVADDIA
                     LLIQHEQLAWVAAHLGVTDAVIMDGNDIRWFEIGPNNTWHNLLWINRFYTLAGVAVDE
                     AAMRAAFNAMLAHFNDAWPTYYRNLSVHEAVRFGSFLDNGAAEMGDYAVNETGHFLEA
                     DSLDINAVSTRFLYRIKPFAS"
BASE COUNT          476 a          469 c          445 g          334 t
ORIGIN      
        1 caccgtaaaa caccaccagg gatccatcta aagtacggcg cgcggattgg gccaccgacg
       61 cagtactaaa ttcatcactc cagcgtaacc cgctgggtaa aacaaggccg tctaactgga
      121 tcatgttgaa gttcgatgcg ggaatggcgg ggaaaaatga ataactcaat aaggaaaaaa
      181 agaatcaaaa aaattaaaaa ttaaaaatta aaaattaaaa aaacgaaatt aagagcgagc
      241 acaaaagcga gcaaaaaaat cgagtgacca ttaaagacac acaaaaccat aaattaaaaa
      301 tcgcctttag cttgcaaagg gtttgatgcg atacaaaaat cgtgtgctca cggcattaat
      361 atccaagctg tccgcctcca aaaaatgacc cgtttcattc actgcatagt cacccatttc
      421 agccgcgcca ttatctaaaa aactgccaaa acgcacggcc tcatgcacgc ttaaattgcg
      481 gtagtaagtc ggccaggcat cattgaagtg ggcgagcatg gcgttaaacg ccgcgcgcat
      541 agccgcttcg tcgaccgcaa ctccagcgag ggtataaaac cggttaatcc acagcaggtt
      601 gtgccaggta ttattcggtc cgatttcaaa ccagcggata tcgttaccat ccatgatcac
      661 agcgtcggtg acccccaagt gagccgccac ccaggccagc tgctcgtgtt ggatgagcag
      721 ggcaatatca tcagccacca cccaaagggt ttcaaatccc tcggcactga gtttttcccc
      781 tcggcacatg atgggcattt gctgagcctg ggaccaaggc cgcttatgcc aggggctggt
      841 atccaccggt gcggtatcgt agccctgggc aaaataggta gcgggatcga gcatggaata
      901 cgcgggcagg cgggttgcag gcgttcgctc caggattggg tcatcggcag tgttgaaatc
      961 gtattcgtac acccctgtgc gtgaaaaccg tttgatggct gaccagcgcg gaataaagcg
     1021 catttctaag tccgtggggt taaattgcca gcgcagaccc gcttgcagag gcggtaattc
     1081 cggtggaata caatcggcct gatccggtgc cgtcaaatac acccgaaaca acacctggca
     1141 ttccccgtct gccatgcgca taaactcatt gctccagttt cgatgccagc catagagagg
     1201 cgaaaaaggg tgcagtaggt aggcattcca gttgcccatg ccggacaccg ctgtggcgcc
     1261 gcttacatag cgggtttgcc cagcggccag ggttatccag gcctgccagc ccgccgcctg
     1321 cacgattaat cccaccgtaa aatccagacg gttggtcagt tggaccaccc agcgaaaggt
     1381 ggtgaggttg tgaagctcaa acgccacctc gaacccaatc gcccggttga ggatcggcgt
     1441 caccggatac agcaactcaa acgggtccat gatcccgcgt gtgctgccag ggatatacgc
     1501 cgtcacactg ggccaatggc gcaaccagat cggcttccag gacatacggg tatcgccctc
     1561 gttgcccaga gcgttactca ggacaaaacg cggctgggtg tcatagccat aggccaataa
     1621 cagcacccgc aggcgaaaat tcccccagcc ggacagaccc aactcagcgg ggtttaaggc
     1681 gggcaggtga taaataaatt gcgcctgatc atgccggatc acgc
//
LOCUS       DAOQGS010000771         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50560_length_1719_cov_3.352163, whole genome shotgun sequence.
ACCESSION   DAOQGS010000771 DAOQGS010000000
VERSION     DAOQGS010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50560_length_1719_cov_3.352163"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKZ52_18685"
     CDS             complement(<1..728)
                     /locus_tag="PKZ52_18685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNG61854.1"
                     /translation="MIDLSVQNATAHKAATSAVSNDKNSAFSFRPNPLAIIIASLGLG
                     FSAVSFAADPAPNTTSTNQAAKNGDAKAAAKSDEGKKNQPKKSVTLSTITVRSRNRVE
                     KLQDVPLSVSVVGGQELERLNATDISAVTQRLSNITWNQGNQRTSSLSIRGIGKQGQT
                     EAQDPPVGLIVDGVNYAYNALASSYDFVDVDTVEVARGPQGTLLGKNASIGVINVTTK
                     KPTFKPEAYYSVTLGQWDTVLGRFA"
BASE COUNT          426 a          375 c          428 g          490 t
ORIGIN      
        1 gcaaagcggc cgagtactgt atcccattgg cctagagtga ccgaataata agcctcgggt
       61 ttaaaggtag gttttttggt ggtgacatta atcaccccga tgctcgcgtt tttgccgagc
      121 agtgttcctt gcgggccgcg ggcgacttct acggtatcca catccacgaa atcgtaactg
      181 gaggccaggg cgttataggc gtagttaacg ccatccacaa ttaaacctac tggcgggtct
      241 tgggcttcgg tttggccttg tttgccgata ccgcgaatgg ataagctgct ggtgcgttgg
      301 ttgccttggt tccaggtaat atttgagagg cgctgagtaa cggcgctaat atcggtggca
      361 tttaagcgtt ctaattcttg gccgcctacc acagaaaccg acaggggcac atcttgtaat
      421 ttctccactc ggttacggct gcgcacagta attgtactga gggtaacgga ttttttaggt
      481 tgattttttt tgccttcgtc agattttgca gctgctttag cgtcgccatt tttagctgct
      541 tgattggttg acgtggtatt aggtgctgga tccgcagcga agctcacagc gctaaatcct
      601 aaccctaagc ttgcgataat aatggcaagg ggattgggcc taaagctaaa cgcagaattt
      661 ttatcattgg atactgcgct tgttgcagct ttatgcgcgg tggcattttg tacactaagg
      721 tcgatcattg gtcactctct gattgtataa aacatatatg gcggggtgtt ttggcaacat
      781 gtatgccatt acgaactttg ctggtttatt tttaaataga ggcctgttga taaaaaatca
      841 ttacagaata gaagcgtctt aaaattgatg tctaaagacg gcaattgttt tgccccacgg
      901 cttgggtccc gctaaaccaa aaggcccgtg gattgtggga ttggctgcgc aaacggctat
      961 ttatcaagat cccagggatg gccgctattt aagcagcaag tgtcgcctta actgcgcgag
     1021 catgaattta aactcgcact catattgttt gcctgcttaa aaaaataccc cacctatagg
     1081 ggctatccat ggtgttatag atatttctag ctgttgaata aataacacct gtgccgtcat
     1141 ctgcacactc aggtccatga atttgcctgc ttttgcgcag tggctatccg gctgcaggac
     1201 ggctgcaggt cttgtggcgt gttattaatt tagcaattgt cccagcctat tttacttcgt
     1261 cgctaaacga ggggccccag agggagccca atggttattt tcaatgcgga aaattcctaa
     1321 aaatgctgtt gcggctttaa cagcctgcca aaaactctgc ttaagaagca acagagctgt
     1381 ttaaaaaaca tagcttcttt atatgtagat aaagcccgcc tgggtttggc ttgcttccga
     1441 atcggcacaa aaaccgctaa ctggcttaaa aataaggatt tttttaatgc gcttagattt
     1501 tggcgcgcag gttgcagtgg gcctgcagag caacctaacc ctgccagttt tgcagcatgt
     1561 tattagtcgg ttgctgcaaa ttgtcttaat gcaaaatccc tgagggaaaa taaatgaaaa
     1621 agaaacacct gtacttagcg gtgctattaa gccttgcaac accctgggca accgctggcg
     1681 atgacgataa ggctgccgat gacaaaggcg ctgatgcga
//
LOCUS       DAOQGS010000772         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50736_length_1715_cov_4.563253, whole genome shotgun sequence.
ACCESSION   DAOQGS010000772 DAOQGS010000000
VERSION     DAOQGS010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50736_length_1715_cov_4.563253"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PKZ52_18690"
     CDS             <1..976
                     /locus_tag="PKZ52_18690"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNG61855.1"
                     /translation="RERNPAGREPITLRAGWRPDDGTGFDNAGFLSFRGASNPRAAEK
                     SPLKAYQRIVGAVGSGTQSQQDALALRRNSINDVLRTQINALMASPALSADDRQRLQQ
                     HLDSVRDLEIAVTQLALDEALLADMAAVDKNVLGMENHPKVIRCHLALLAFAVTSGYS
                     LTGTLKVGDRIDSHQWIINGNKLPQFHMISHRNLSDAEFGAAIPNATELHRQVDRIHA
                     ADFLYLCGLLRDVNTPTGPLIDQGFAVWTNQMATGWHRHDNQPFVIVGNAGGYLKSGQ
                     YIDAGGVKLNKMLNTLLTAAGVKGTGGGPVTNFGDASLPGGLIASMIA"
     gene            1156..1715
                     /locus_tag="PKZ52_18695"
                     /pseudo
     CDS             1156..1715
                     /locus_tag="PKZ52_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
BASE COUNT          387 a          505 c          467 g          356 t
ORIGIN      
        1 ccgtgagcgc aatccggcag ggcgcgagcc tatcaccctg cgcgcagggt ggcgcccgga
       61 cgacggcacc ggcttcgata acgccgggtt tctgtccttt cgcggcgcca gcaacccccg
      121 cgctgcggag aagagccccc tcaaagccta ccagcgcata gtgggcgcgg tcggttcggg
      181 cacccagtcc cagcaggacg cgttggcatt gcgacgcaac agtatcaacg atgtgctgcg
      241 aacccagatc aatgccttga tggcaagccc cgccctcagc gccgacgacc ggcagcgcct
      301 tcaacagcac ctggattccg tgcgcgattt agagatcgca gtcacccaac tggcgctgga
      361 tgaagcactc cttgccgaca tggccgccgt ggacaaaaac gtactgggca tggagaacca
      421 tcccaaggtc atacgctgcc acctggcgct cctggcgttc gccgttacct cgggatacag
      481 cttgaccgga accctcaagg tgggggatcg catcgactcc catcaatgga tcattaacgg
      541 taacaagctg ccgcagttcc atatgatttc ccaccgcaat ctaagcgacg ccgaatttgg
      601 tgcagccatt cccaatgcca ccgaactaca ccggcaagtc gatcgcattc acgcggcgga
      661 ctttctctac ctctgcgggc tactgcgaga tgtgaacacg ccgacgggcc ccctcatcga
      721 ccagggcttt gctgtctgga ccaatcaaat ggccaccggc tggcataggc acgacaacca
      781 gcccttcgtc atcgtcggca acgcgggcgg ataccttaaa tccgggcaat acatcgacgc
      841 gggcggcgtc aagctcaaca agatgctcaa tacattgcta acagctgcag gggttaaagg
      901 cacaggtggg ggcccggtga cgaacttcgg cgatgcttcg ctgcctggcg ggctgattgc
      961 gtcgatgatt gcgtaacgtg caatagcccg gagagcacag ttttagaggg cttttttagg
     1021 gttaagagat cactactgaa ttcaacaaaa atagagcggt taacattcat gaatagttca
     1081 ataacaaaca aacggctttt acctctggcg cttgtggcca gcagtatttg ggttactggc
     1141 tgtggcggtg aaaataccag ttccagtagc agctctagct cctcaagttc ttctagctct
     1201 tcgagctcct ctagttcaac cagctcatca ggaaagagta gctccagctc gtcaagctcc
     1261 agtagttcat cctcgtcatc gagttcgtct agcagctcat ccagctcaag tggcttggct
     1321 gtacagggta atttggtggt ggcggtgaac gccggtggca gtgccataac ggttgcgggt
     1381 gtgccgttta aagcggatca gtactatcgc ggcggcacgg tcaataccac cacagaccca
     1441 attgcaggta cagaaaacga cagtttgttt cagtccgagc gctacggcac ctattcctat
     1501 gaagtccccg tctcaagggc aacctacgat gtcgagctgt attttaacga gttgtatcaa
     1561 accacagcag ggtcccgcgc ctttagtgtt gcggtagaag gccagccgct gtttagcaac
     1621 ctagatctat tcactacagc cggccacgat accgcctaca cccgggtgat taaggacgtg
     1681 gccgtaagcg atggcagcct gagtatttcc ctgtc
//
LOCUS       DAOQGS010000773         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50817_length_1714_cov_2.749849, whole genome shotgun sequence.
ACCESSION   DAOQGS010000773 DAOQGS010000000
VERSION     DAOQGS010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50817_length_1714_cov_2.749849"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKZ52_18700"
     CDS             complement(<1..410)
                     /locus_tag="PKZ52_18700"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNG61856.1"
                     /translation="MMRVIFAVATFACALHAWAGCNSALPKSTPDTQFTLKASGAIVR
                     DNKTGLQWQRCSLGQVWNPVQNSCDATTTARWFNWQQALNAAGENRLAGFDNWRLPNK
                     KELASILELSCSQPAINSRLFPNTAAGRYWTSTPV"
     gene            complement(410..1207)
                     /locus_tag="PKZ52_18705"
     CDS             complement(410..1207)
                     /locus_tag="PKZ52_18705"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNG61857.1"
                     /translation="MLQKILIFSASSLLVACGGTPIAIDPPPTAPLNDTGVSQCRSAD
                     ELTKCPQNNNARQDAEQGRDAEALAGRLSKVGGGVAGFDFTKLAMNGLPLARQNQTWQ
                     VNGSESAGTRWFCVKDNHTGLIWEVKSNTEGDLHYSGDTFSWYNPDTKINAGNPGGQN
                     LGFCHQAPCDTWGFIARVNSEKLCGRSNWRLPTAVELLSIIDQSQINPPVDTQYFPNL
                     SFNAHWSSQTHAERLNAAWYVYFTAGDNGAIAKTSVANALLVSGGEE"
BASE COUNT          393 a          435 c          421 g          465 t
ORIGIN      
        1 acaggggtgg atgtccagta gcggcccgcc gccgtattgg gaaataggcg gctgttaata
       61 gccggttggc tacaactgag ttctagtatg gacgctaatt cttttttatt gggtaggcgc
      121 caattatcaa acccagctaa gcgattttca cccgccgcat ttaatgcctg ctgccaatta
      181 aaccagcgcg cggttgtagt tgcatcgcag ctgttttgca ctgggttcca cacttgccct
      241 aagctgcagc gctgccactg caaaccggtt ttattatccc gcacaattgc accgctggct
      301 tttagcgtaa attgtgtatc cggcgtgctt ttgggtaggg cgctattaca gcctgcccag
      361 gcgtgaagtg cgcaagcgaa tgtggctact gcaaaaataa cacgcatcat tattcctccc
      421 cgccactgac taataaggca ttggcaacac tggttttcgc aattgcgcca ttatcacccg
      481 ccgtaaaata gacataccaa gccgcattta accgctcggc atgggtttgg gagctccagt
      541 gagcattaaa tgaaagatta ggaaaatact gggtatccac cggtgggtta atttggcttt
      601 ggtcgataat ggataataat tccacggcgg tgggcaagcg ccaattgctg cgcccgcaga
      661 gtttttcgct attgacccgc gcaataaacc cccaggtatc acagggcgct tgatggcaaa
      721 aacctaaatt ctgcccacct gggttgcctg catttatttt tgtatctggg ttataccaac
      781 taaaagtatc gccgctgtaa tgcaggtcgc cctcagtatt ggatttcacc tcccaaatta
      841 aacccgtgtg gttgtcttta acacaaaacc agcgtgtgcc tgcggattca ctgccgttta
      901 cttgccaggt ttgattttgc cgggcaaggg gcaggccgtt catcgccaat ttagtgaaat
      961 caaaaccggc aaccccaccg cccactttag ataagcgccc cgccaaagcc tcggcatctc
     1021 ggccctgttc ggcatcctgg cgagcgttat tgttttgcgg gcatttagtt aattcatccg
     1081 cactgcggca ttggcttaca cctgtatcgt ttaacggcgc tgtagggggt gggtcaatgg
     1141 caattggtgt gccgccgcag gcaaccaata gggagctggc gctaaagatc aaaatttttt
     1201 gcagcatagg tgtatccgct tgtttaaaaa cccgttactg ctcattttat tggctggata
     1261 taagttttgc gtggaacttg cgcacggaat aatgccgata tttttgtctg ttttatcggt
     1321 ttaaaaacgg atttttgaag gggttattaa cccggcggca aaatagatcg tcctgatcta
     1381 ttttgccttc gctccgctaa acttgcgaaa atgcgcggat ttttttgatt tagcggagcg
     1441 cgcggcggct tgcgccgcag gtttggcatc cttgccgcaa ttattaacct ggcataaaaa
     1501 tccgctgggt taataatacc tgctgcactc taaaaattgg ttttgaacgg cgtgcatcct
     1561 aaaattcctt gcttatgggc ggaagctcag tgcgccagcc gggggcacgg cctatttatt
     1621 tccatgggcg agtatttttt ttacttatat ggctgcagct ggcaacaaaa aacaccgcca
     1681 cccaccaccc caccccttcg gggcacacct ccgc
//
LOCUS       DAOQGS010000774         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50939_length_1711_cov_4.062198, whole genome shotgun sequence.
ACCESSION   DAOQGS010000774 DAOQGS010000000
VERSION     DAOQGS010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50939_length_1711_cov_4.062198"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKZ52_18710"
     CDS             <1..1098
                     /locus_tag="PKZ52_18710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG61858.1"
                     /translation="GRPFAADLAQVEGQAARNRAMLTNAQADLARFTSLGQQTAISAQ
                     QIDAQKSLVEQYRGLVQADQGAVEKARLQLEFTQIRAPISGRIGLRRVDPGNNINAQD
                     PEGLALITEVNPIDVVFALPGELAGELAAQLHTAQAQKRRLTVEAWDKANKTRLSLGE
                     LLSLDNQIDASTGAIKLKARFSNDAGLLFANQFVNVRVQIQQLKQALVVPTRAILRGS
                     EGPFVYALSHADGVGQPDRAEQGDRSKPGVRSKPGDKTAQASAKPGDSNKPTTSPKQA
                     EAGNQASAPRKEPSSGPIAAVRPVTVGVSQGEYTQISSGLQAGDEVVISGADRLREGI
                     QVRVANSTASGPSGAAITDAQAEVPPKARAF"
     gene            1101..>1711
                     /locus_tag="PKZ52_18715"
     CDS             1101..>1711
                     /locus_tag="PKZ52_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG61859.1"
                     /translation="MSQTSPNPSQLFIERPVATSLLMVAILLAGILAYQLLPQAALPQ
                     VEYPSIQITTFYPGASPELMTRVVTAPLERQFGAIAGLQQMSSASASGVSQITLQFEL
                     ALRLDIAEQSVQAAINSALNFLPRDLPAPPVYSKINPADMPVLTLAITSATLPTTQVQ
                     DWADTYLALKIAQVSGVGQVALSGGQRPAVRVQVNPQALASHG"
BASE COUNT          387 a          533 c          482 g          309 t
ORIGIN      
        1 gggcgcccat ttgcggctga tttagcccag gttgaaggcc aggcggcgcg caaccgtgca
       61 atgcttacca acgcccaagc ggaccttgcg cgcttcacct cactgggcca acaaaccgcc
      121 atctccgccc agcaaataga cgcccaaaaa tccttggtag agcagtatcg cggcttagtg
      181 caagccgatc agggcgcagt tgagaaggcc cgcctgcaac tggaattcac gcaaattcgc
      241 gcgcctattt caggccgcat tggcttgcgg cgggtggacc caggcaacaa tatcaacgcc
      301 caggacccag agggcctcgc cctcattacc gaggttaacc ccattgatgt ggtatttgcc
      361 ctccccggtg aactcgccgg tgaactggca gcgcagctac acactgccca agcgcaaaaa
      421 cgccgcttaa cggttgaggc ttgggacaaa gccaataaaa cccgcctgag cctgggcgag
      481 ttgttaagcc tggataatca aatcgatgcc agcaccggcg ctattaaact caaagcgcgc
      541 tttagcaatg acgccgggct gttattcgcc aaccaattcg tcaatgtgcg ggtgcaaatt
      601 caacagctga agcaagcgct ggtggtgcca actcgggcca ttctgcgcgg tagtgaaggc
      661 ccctttgtgt atgccctcag ccacgccgat ggtgtaggac aaccagatcg cgccgagcaa
      721 ggcgaccggt caaagccagg cgttaggtca aagccaggtg ataaaaccgc gcaggcaagc
      781 gccaaacccg gcgacagcaa taaacccacc acaagcccca aacaggcaga ggccggcaat
      841 caggccagtg ctccaagaaa agaacccagc tcaggcccaa ttgcagcagt gaggcccgtg
      901 accgtcgggg tgagccaagg agagtacacc caaatatcca gtggcctgca ggcaggagat
      961 gaagtagtga tcagcggcgc cgatcggctg cgcgaaggca tccaggtgcg ggtggctaac
     1021 tcaactgcct caggcccctc cggtgccgcg attactgatg ctcaggctga agtacccccc
     1081 aaggcgcgag ccttttaatc atgagccaaa caagccccaa cccttctcag ctgtttatcg
     1141 agcggccggt tgccacttct ttgttgatgg tggcaatttt attggcgggt attctggcct
     1201 accagctgct gccccaggcg gccctgcccc aggtggagta cccgagtatt caaatcacca
     1261 ccttttatcc cggggcgagc ccagagttga tgacccgggt ggtgaccgct ccgctagagc
     1321 ggcagttcgg cgccattgca ggcttgcaac agatgtcctc ggccagtgcc agcggggtat
     1381 cgcaaatcac cttgcaattt gaactggccc tgcgcttaga catagcagag cagtcggtgc
     1441 aggcggcgat taattccgcg ctgaattttc tgccccgcga cctgcccgcc ccccctgtgt
     1501 acagcaagat caacccggcg gatatgccag tgctcactct cgccatcacg tcagcaacct
     1561 tgcccactac ccaggtgcaa gattgggcgg atacctattt agcactgaaa atcgctcagg
     1621 tcagtggtgt ggggcaagtg gccttgagcg gcggccagcg cccggcggtg cgcgtacagg
     1681 tcaaccccca ggcactggcc tcccacggct t
//
LOCUS       DAOQGS010000775         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_50992_length_1710_cov_3.570997, whole genome shotgun sequence.
ACCESSION   DAOQGS010000775 DAOQGS010000000
VERSION     DAOQGS010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50992_length_1710_cov_3.570997"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKZ52_18720"
     CDS             complement(<1..766)
                     /locus_tag="PKZ52_18720"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HNG61860.1"
                     /translation="MTDLAKVVRLEVTNERMTKARLTQLPKELHHCLEQARGLLLPLL
                     ETCLDRLEDRLFNLADKSASPHEQNTYFNAMRRVRQQRSVFLKRFGARLQEAFADLDG
                     TQAPAAAPFTADTLALLRPEDLDELIAVEAMITKAQRSFADGITQLSQRIDALVSTPV
                     YARNNPVGPEALCQVLSSSALQLDIPAPIKLILLQAFDDTVLSALGPVYQALVEPLKN
                     LRANKTAVPSADVTLNTQAQTTPAQHNSFDAELASVL"
     gene            complement(1326..>1710)
                     /locus_tag="PKZ52_18725"
     CDS             complement(1326..>1710)
                     /locus_tag="PKZ52_18725"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNG61861.1"
                     /translation="IPSAAQLKLSGRLLGQTPWKGSVFAGQQQIEVSAPSYEPQTMLT
                     TVAPGQQVSLSVLLSPQLVAEPVPRGSAPLLPQTRRPLWRVVTGAAAMGVGILMIGIG
                     GSGLAADGTCVPPLQPPAPAPQPRP"
BASE COUNT          397 a          495 c          475 g          343 t
ORIGIN      
        1 ccagcactga ggccaattcc gcatcaaagg agttgtgctg cgcaggtgtg gtttgtgcct
       61 gtgtattgag tgttacatct gccgagggca cagcggtttt atttgcgcgc agatttttta
      121 agggctcaac aagcgcctga taaaccggcc ccaaggcaga taaaacggtg tcatcaaacg
      181 cctgcagcaa aattaattta ataggtgcgg gaatatccaa ctgcagggct gagctgctta
      241 atacctggca gagggcctca ggccccaccg gattattgcg ggcgtacaca ggggtactta
      301 ccaaagcgtc tatgcgttgg gacaattggg taatgccgtc ggcaaaactg cgttgggctt
      361 tggtaatcat ggcctccacg gcaattaatt cgtccaaatc ctccggccgt aaaagcgcga
      421 gggtatccgc cgtgaagggt gcggcagcgg gcgcctgtgt gccgtctagg tcggcaaaag
      481 cctcttgtaa acgggcgcca aagcgtttta aaaacacgct gcgctgctgg cgcacacgcc
      541 gcatggcatt aaagtaggtg ttttgttcgt ggggggaagc ggatttatcg gccaggttaa
      601 acaggcgatc ctctaaccga tccaggcagg tttcaagcag ggggagcagg agcccgcggg
      661 cctgctctag gcagtgatgc agctctttcg ggagctgggt taagcgcgcc ttagtcatac
      721 gctcattggt tacctccaaa cgcacaacct tcgcaagatc tgtcatagtt tttctccgaa
      781 cacaggcagg cgctggcgcc atacccggca gggctgatga tcggccaagt gccctgcata
      841 cagcttggac attggcgatt aatcagcgcg caggcaaata agacagcagc gcttcagtct
      901 tcagtctaga cattcaccgg gtatgaattc agcccaaagt attagtaagc gacaatgtac
      961 cataatcaaa tctaaaaaat gtgacgggtt aatactttaa agcccccacc taagaacatt
     1021 ttttgtaaga tcgccgccag cctataaccg tacccctaat cctatgcagc ccccgcacct
     1081 aaaattcgcc cgctggctca gtaacggcgc ttggctcgct ttactggccc tactcaccct
     1141 ctatttcgcg ggcaaggagc gcgcgcagat caacccgaac caggaggtga agagcaccgg
     1201 ggacggcggc tttgccgaag tgcaattaaa agctaacaac catcatcact atttagccaa
     1261 cggcaccatc aaccgcaaac ccgtcacttt tatgattgat acaggcgcct cagacgtggc
     1321 gatacctaag gccttggctg aggagctggg gctggtggct ggagaggcgg cacgcaggtt
     1381 ccatcggcag ccagccccga accaccgatc ccaatcatca agatgccgac gcccatcgcc
     1441 gctgcgccgg tcaccacgcg ccacagcgga cgacgcgtct gtggaagcag cggcgcactg
     1501 ccacgcggca ctggctcggc gacaagctgc ggcgacagca gcaccgacag cgagacctgc
     1561 tgaccgggag ccactgtcgt cagcatcgtc tgtggctcgt agctcggcgc cgacacttcg
     1621 atctgctgct gcccggcaaa caccgaccct ttccacggcg tctgacccag cagccgtccg
     1681 ctcagcttga gctgcgccgc cgacggaatg
//
LOCUS       DAOQGS010000776         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_51118_length_1707_cov_4.467918, whole genome shotgun sequence.
ACCESSION   DAOQGS010000776 DAOQGS010000000
VERSION     DAOQGS010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51118_length_1707_cov_4.467918"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKZ52_18730"
     CDS             <1..208
                     /locus_tag="PKZ52_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNG61862.1"
                     /translation="DVLKAFVDSPLPVEISAPISVPSLERVEWEHIQKALQDCQGNIS
                     AAARLLGMHRRTLQRKLFKRPVAR"
     gene            complement(334..1611)
                     /locus_tag="PKZ52_18735"
     CDS             complement(334..1611)
                     /locus_tag="PKZ52_18735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG61863.1"
                     /translation="MNANSIPTHEPPRQHLRQLTVIRSIVLGFFWVVFIASFWVDSLA
                     LPYTQVMSILGVFSSIHLLTLIRLKHALPVTNSEFFIQLLIDVFCLNLVFYFSGGATN
                     PFISYLLVAVCICAATLPWRHTWIITALCVIAYSLLLFFHIPLQIFTMDHMQMQSQLN
                     WHILGMWFNFFVSALLITYFVVKMANTVRQQELRINQMREDELRNEQVIAVATLAAGA
                     AHEMNTPLATMTVLLGELRDEHSANTALQEDLALLSRQVHQCAQSLKHIVQDAAAASL
                     GQLKQHSAAEYCQSILDRWQLMRPEVHYTLKVSEELTTHTITYDSKLDHAIINLLNNA
                     ADASPEHISLAITLDEQNLIWTISDAGSGIQSEVSALLGKTTITTKDKGLGLGMLLTH
                     TTISRYGGSVTQTANDPQGTITTLRLPLNSAIY"
BASE COUNT          417 a          366 c          465 g          459 t
ORIGIN      
        1 ggatgtatta aaagcatttg ttgattcacc tctgcctgta gagataagcg cgccgatatc
       61 tgtgccctcg cttgagcgag tggagtggga gcatattcaa aaagccttgc aggattgcca
      121 gggcaatatt tccgccgctg cgcgcttgtt ggggatgcat cggcgcacct tacaacgcaa
      181 attatttaaa cggcctgtgg cgcgataggg ctttttacta ccgaggtgcc agcaatgaat
      241 acccatcatt tcagacacgc cgtaaataca tccttgtagg ctcgtcaaaa aggtccctct
      301 ttttggcggt ctgaaatcat tgcttccgta cagttagtaa atggcggaat ttagcggcag
      361 ccgaagcgtg gtaattgtgc cctgcggatc attggctgtc tgcgttacgc tgcctccgta
      421 gcggctgatt gtggtgtggg tgagcaacat gcccagcccc aggcccttat ctttagtggt
      481 aatggtggtt ttgccgagca gtgccgatac ttctgattga ataccgctgc cagcgtcgct
      541 gatcgtccaa attaaattct gttcgtccag tgtgatggcg aggctgatgt gctcggggct
      601 ggcatcggcg gcattattga gtaaattaat aatggcgtga tcaagctttg agtcataggt
      661 gatggtgtgt gtggttaatt cttctgacac cttaagcgtg taatgcacct cagggcgcat
      721 caattgccag cgatctaaaa ttgattggca atattctgca gcgctgtgtt gttttaattg
      781 cccaaggctt gctgctgcgg cgtcttgcac tatgtgtttt aggctttgcg cgcactggtg
      841 tacctggcgg gacaatagcg ctaaatcttc ttgtaaggcg gtattggctg agtgttcatc
      901 gcgcaattcg cctaataaca ctgtcatagt ggccaaaggt gtgttcattt catgggcggc
      961 gccagcggcc agggtggcca ccgcaatgac ttgttcgttg cgcagttcgt cttcacgcat
     1021 ttggttaatg cgcagttcct gctggcgtac cgtgttggcc atttttacta cgaaataggt
     1081 aattaataat gcgctgacga aaaaattaaa ccacatccct aaaatatgcc agttcagttg
     1141 cgactgcatc tgcatatggt ccatggtaaa aatttgcagc ggaatatgga aaaataacaa
     1201 tagggaatag gcgatgacgc agagcgcggt gatgatccag gtgtggcgcc agggcagggt
     1261 ggcggcgcag atgcacaccg ccaccaataa ataggagata aatggattgg tggcgccgcc
     1321 gctgaaataa aacaccaggt taaggcaaaa tacgtcaatt aacagttgga taaaaaattc
     1381 actgttggta acgggcagcg cgtgttttag gcgtatcagc gtgagcaggt ggatgctgct
     1441 gaatacgcct aaaatactca ttacttgcgt gtagggtaac gccaggctgt caacccaaaa
     1501 actcgctata aacacaaccc agaaaaaccc taaaacaata ctgcgaataa ccgtgagctg
     1561 gcgcaggtgt tgacggggtg gctcgtgtgt tgggatggaa ttggcattca tgaatctatt
     1621 ttcaaggaaa taattctggt tgcgggtgtt gtagattctc ttggtgagta gctattcgtc
     1681 ataaaaatat cgaacgcggt atttttg
//
LOCUS       DAOQGS010000777         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_51128_length_1707_cov_3.724576, whole genome shotgun sequence.
ACCESSION   DAOQGS010000777 DAOQGS010000000
VERSION     DAOQGS010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51128_length_1707_cov_3.724576"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKZ52_18740"
     CDS             complement(<1..877)
                     /locus_tag="PKZ52_18740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNG61864.1"
                     /translation="MDFDQFIDRTHTSSEKYGARKRVFGREDVMPLWVADMDFACPAS
                     ITQALHERVQHPIYGYTQYPQEMKSVLINWLARRHQCAVEPDWISFAPGVVPSLHAAA
                     LGLLKSDEALLIQPPVYPPFFYVAKNTARALLHNPLIPQNGRYAIDFADLEHKAAQAK
                     LLILCSPHNPVGRVWEPDELRALLRIAKRHNLYVFSDEIHQDLILPGFKHTMLLKFIN
                     DPELGDFIASNTVTAVAPSKTFNIPGMGLSALITPNPQLRQKLTQAFDLLHAGNFNPL
                     SITAFIAAYQNNDNWL"
     gene            complement(1094..>1707)
                     /locus_tag="PKZ52_18745"
     CDS             complement(1094..>1707)
                     /locus_tag="PKZ52_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044272 - sulfur compound biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative 4-mercaptohistidine
                     N1-methyltransferase"
                     /protein_id="HNG61865.1"
                     /translation="RSKALDLGCASGRASFELARDFASVIGIDFSARFINQGVQLAEQ
                     GLLRYTLVEEGELVSYKSRSLEQLGLAEVKDKVAFFQGDACNLRAEFTGFDFILAANL
                     IDRLYKPSKFLTEVHLRLNIGGLLLIASPYTWLEEHTPKEEWIGGFKRDGENYSTLDG
                     LRQLLGGHFRQVAGPEGVPFVIRETKRKYQHSVSEVTLWERIK"
BASE COUNT          418 a          403 c          463 g          423 t
ORIGIN      
        1 ccagccaatt gtcattattc tggtaggcgg cgataaatgc ggtgatgctt agggggttaa
       61 aattaccggc gtgtaataaa tcaaaggcct gggtgagttt ttgccgcagt tgagggttgg
      121 gggtaattaa ggcggatagg cccatgccgg ggatattaaa ggttttgctg ggggcgacgg
      181 cggtgacagt attgctggcg ataaaatccc ccagctctgg gtcgttaata aatttgagca
      241 gcattgtgtg cttaaagccg ggcaatatta aatcctggtg aatttcatca gagaagacat
      301 ataaattgtg ccgtttggcg atgcgcaaca gcgcgcgcaa ttcatccggt tcccacacgc
      361 ggcccacagg gttatgaggg gagcaaagga ttaataattt ggcctgggcg gctttatgtt
      421 ctaaatcggc aaaatctatt gcgtagcggc cattttgggg aataagtgga ttatgcagca
      481 gcgctcgcgc ggtatttttg gctacgtaaa aaaaaggcgg gtacacgggc ggttgaatta
      541 atagtgcttc atcgcttttt aataatccta aggcagctgc gtgtaaggat ggcacaacgc
      601 ccggggcaaa gctgatccaa tccggctcta ccgcgcactg gtgcctgcga gcgagccaat
      661 ttattaatac ggatttcatt tcctgcgggt attgggtgta gccataaata gggtgttgga
      721 cacgctcgtg cagcgcttgg gtaatagacg cggggcaggc aaaatccata tcagccaccc
      781 acaggggcat tacgtcttca cggccaaata cacgcttgcg ggcgccgtat ttttcgctgc
      841 tggtgtgggt gcggtcgatg aattgatcaa aatccacggg gtgctcctta agtgagcggg
      901 tgggtacttt aaataattgt gcgatattac cgctgccata aacccctccc caaccctccc
      961 cttaaaaagg ggagggagca gagattctaa ggtgcgggct cagtgccttg gctgcaaact
     1021 ttttacttgt cttgcattta aaaatgagcg gcaatgtgca agcgattgcc gctcgatgcg
     1081 cctggatacc gtcttattta attcgctccc acaaagtaac ttccgacacg ctgtgctgat
     1141 atttgcgttt ggtttcgcga atcacaaagg gcacgccctc tggccctgcc acttggcgaa
     1201 agtgcccacc caacaattgg cgcaggccat ctagagtgga ataattttcg ccgtcccgtt
     1261 taaagccgcc aatccattct tctttcgggg tgtgttcttc caaccaggtg taaggtgaag
     1321 caattaataa caggccgccg atattgaggc gcagatgtac ttcggtgagg aatttgctgg
     1381 gtttgtacag gcggtcgatt aaattggcgg cgagaataaa atcaaagccg gtaaattccg
     1441 cccgcaggtt gcaggcatca ccttggaaaa acgccacttt gtcttttact tcagccagcc
     1501 ccaattgctc caaactgcgg gatttataac tgactaattc gccctcttcc accagggtat
     1561 accgcaacaa accctgttca gccaattgca ccccctggtt aataaaccgg gcggaaaaat
     1621 caatgccgat aaccgaggca aaatcgcggg ctaattcaaa gcttgcacgg ccggaggcgc
     1681 agcctaaatc cagcgctttg ctgcggg
//
LOCUS       DAOQGS010000778         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_51292_length_1704_cov_4.486355, whole genome shotgun sequence.
ACCESSION   DAOQGS010000778 DAOQGS010000000
VERSION     DAOQGS010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51292_length_1704_cov_4.486355"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKZ52_18750"
     CDS             <1..361
                     /locus_tag="PKZ52_18750"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNG61866.1"
                     /translation="VYDPDLEQVTVRKNPGDSDNSPARLLSGDVAKMAPLFKVSEKQN
                     GGEQEFTLTPKKPEKEQLKQIQFTFTGGSLATLAFEDKLGQTTRLILSNTAANTPVDM
                     NLFTFVPKPGTDVIEND"
     gene            354..1700
                     /locus_tag="PKZ52_18755"
     CDS             354..1700
                     /locus_tag="PKZ52_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNG61867.1"
                     /translation="MTEDLFTQAAKPFGPLAARMRPAVLADYIGQEHILGAGKTLRQA
                     LERGQLHSIIFWGPPGVGKTSLARLLAHEINARFIQLSAVLAGVKDIREAVASADYEQ
                     KLHGRKTLLFVDEVHRFNKAQQDAFLPFVEDGTITFVGATTENPSFEVNNALLSRCRV
                     YVLESLKPQELAQLMQRALTDSERGLGNRHLRLTAQAEQGLINAADGDGRRCLNLLEI
                     ASDLVNDGEAIDTNILARVLATDVRRFDKGGDLFYEQISALHKSMRGSDPNASLYWFM
                     RMLDGGCDPLYIARRLVRMASEDVGNADPRALQLCLNAWDVQERLGSPEGELAMAQAL
                     LYLAAAPKSNAVYKAYNECKAQVKSLPSFNVPMHLRNAPTALMKGLDYGKAYRYAHDE
                     PDAFAAGEYYFPEEMPEQDYYRPVPRGLEIQIGEKLKRLAEKNAAARAQTEEGRTT"
BASE COUNT          448 a          433 c          435 g          388 t
ORIGIN      
        1 ggtgtacgac cctgatttag aacaggtaac cgtgcgtaaa aaccctggcg attccgataa
       61 cagcccagcg cgtttattaa gtggtgatgt agccaaaatg gcgccgcttt ttaaagtgag
      121 cgaaaagcaa aacggcggtg aacaggaatt tacgctgacg cctaaaaagc cagaaaaaga
      181 acaactcaaa caaattcaat ttaccttcac cggcggcagc ctggccacct tggcgtttga
      241 agataaatta ggccaaacca cgcgcttaat tctctccaac accgccgcca acacgcctgt
      301 ggatatgaat ttatttacct tcgtgcctaa gcccggcacc gacgttattg agaatgactg
      361 aggatttatt tacccaggcg gccaagccct tcggcccttt ggccgcgcgc atgcggccag
      421 cggtcttagc cgattacatt ggccaggagc atattctcgg cgcgggcaaa accctgcgcc
      481 aagcgctaga gcgcgggcaa ttacattcca ttattttttg ggggccgcca ggggtgggta
      541 aaacatccct tgcgcgccta ttagcccatg aaataaacgc acgttttatt caattatccg
      601 ctgtgttagc aggtgttaaa gatattcgcg aggccgtggc cagcgccgat tacgaacaaa
      661 aactccacgg ccgcaaaaca ctgttatttg tggatgaagt gcaccgcttt aacaaagccc
      721 agcaggatgc atttttaccc tttgtggaag atggcaccat tacctttgtg ggcgccacca
      781 cggaaaaccc ttcctttgaa gttaataatg cgcttttatc ccgctgccgg gtgtatgtgc
      841 tggaatcctt aaagccccaa gaactcgcgc aattaatgca gcgcgcatta accgatagcg
      901 aacggggctt gggcaatcgg catttacgtt taacggctca ggccgaacaa ggcttaatta
      961 atgccgccga tggcgatggc agacgctgtt taaatttatt agaaatagcc agtgatttag
     1021 taaacgatgg ggaagccata gacacgaata ttctcgcgcg cgtactcgca actgacgtgc
     1081 gccgttttga taaaggcggc gatttattct acgaacaaat ttccgccctg cataaatcca
     1141 tgcgcggctc agacccaaac gccagtttat attggtttat gcgtatgctc gatggcggct
     1201 gcgacccttt atacattgct cgccgcctag tgcgcatggc aagcgaagat gtcggcaatg
     1261 ccgacccgcg cgctttacag ttgtgtttaa acgcctggga tgtgcaggag cgtttaggca
     1321 gcccggaagg tgaattagcc atggcccaag cgctgctgta tttagcggcg gcgccaaaaa
     1381 gtaatgcggt gtataaagcc tataacgagt gcaaagcaca ggtaaaaagc ctgcccagtt
     1441 ttaatgtgcc catgcatttg cgcaatgcac ccacggcatt aatgaagggt ttggattatg
     1501 gcaaagccta ccgttacgcc cacgacgaac ccgatgcctt tgctgccggt gagtattatt
     1561 tcccggaaga aatgcccgag caggattatt accgccctgt gcccaggggc ttggaaattc
     1621 aaattggcga aaaattaaag cgcttagcgg aaaaaaacgc tgcagctcgc gcgcaaaccg
     1681 aagaggggcg cacgacatga tgtg
//
LOCUS       DAOQGS010000779         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_51447_length_1701_cov_4.160996, whole genome shotgun sequence.
ACCESSION   DAOQGS010000779 DAOQGS010000000
VERSION     DAOQGS010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51447_length_1701_cov_4.160996"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..>1701)
                     /locus_tag="PKZ52_18760"
     CDS             complement(12..>1701)
                     /locus_tag="PKZ52_18760"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016389194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNG61868.1"
                     /translation="LSLSCFAETVSQLQPTQVQSATIKDVVKMLSTRHYRNLPIDDQL
                     SEAFLDKYLESLDASRMYFLQSDISGFNRFRHQFDDDIKAGNLENGFAIYRTLKQRME
                     SRLEWVIKYLSEPNKKFEFTTQEVIEVERDKSPWPKNAQDADTIWDQRIRLSILNQKL
                     SGKTTEKSVETLLRRYKNQLARIQQQNSEDVFETFINSLTILYDPHTNYFSPRTSENF
                     NISMSLSLEGIGAVLQSEDESTKVVRLVPGGPAARQGQLKPGDKIIGVGQGKDAEPVD
                     VLGWRLDEVVDQIRGKKNTQVKLDVQSAEGQSKQVVITRDTVKLEDQAAQKAVIEIKD
                     GDKVKKIGVINLPTFYIDFKAAKDGDPNYTSTTRDVARLIGELQKENVEGIVLDLRNN
                     GGGSLQEATQLTGLFIDPGLVVQIRDANGQIHRENRSYAEALYKGPLVVMINRLSASA
                     SEIFAGAIQDYQRGLIVGSQSFGKGTVQLLSKLPEGELKLTESKFYRVSGASTQHRGV
                     VPDVFMPNQIDNDEVGESTYNTALPWDQIRPASHESYYDFSQIIPQLTQRRAVL"
BASE COUNT          411 a          390 c          402 g          498 t
ORIGIN      
        1 ggtgaatcaa cctataacac tgccctgcgc tgggttaact gcggaataat ttgcgagaaa
       61 tcatagtaac tttcgtgact ggcggggcga atctgatccc agggcagggc agtgttatag
      121 gttgattcac cgacttcatc attatcaatt tggttgggca taaatacatc tggcaccaca
      181 ccgcgatgct gggtgctggc gccggaaacg cgatagaact tggattccgt taacttaagt
      241 tcgccttctg gcaatttgct cagcaactga actgtgccct taccaaatga ttgggagccc
      301 acaattaaac cccgttgata atcttgaatg gcaccggcaa aaatttctga ggcggaggcg
      361 ctcaagcggt taatcatcac caccaagggg cctttataaa gcgcttctgc gtaggagcga
      421 ttttcgcggt gaatttgtcc attggcatcg cgaatttgta ccaccaaacc tgggtcaata
      481 aacaatcccg tcagttgcgt tgcctcttgc aaagagccgc cgccgttgtt gcgcaggtcc
      541 agaacaatac cctcgacgtt ttctttttgc agctcaccaa ttaagcgcgc tacatctcgg
      601 gtagtgctgg tgtaattagg atcgccgtct ttggcagctt taaagtcgat gtaaaatgtt
      661 ggcaaattaa ttacgccgat ttttttcact ttatcgccgt ctttaatctc tattactgct
      721 ttctgcgcag cttgatcttc aagttttact gtgtcacgag taatgaccac ttgctttgac
      781 tgcccttcag cagactgtac atcaagcttc acttgggtat tctttttacc gcgaatctga
      841 tctaccacct catctaagcg ccagcctaaa acatctactg gctcggcgtc tttaccctga
      901 cctacaccaa taattttatc gccaggtttt aattgccctt ggcgagcggc cgggccgccg
      961 ggcaccaggc gtaccacttt agtgctttca tcttcagact gtaataccgc gcctatgccc
     1021 tctagggaca agctcatgct gatattaaaa ttctctgagg tgcgaggtga aaaataattg
     1081 gtgtgtggat cgtacaaaat agtcaaagag ttaatgaagg tttcaaaaac gtcttcgctg
     1141 ttttgttgtt gaatacgtgc caattgattt ttatagcggc gcaacagagt ttccacggat
     1201 ttttccgtgg ttttgccgga aagtttttgg ttgagaatac tcaaacgaat gcgttgatcc
     1261 caaattgtat cggcatcttg ggcatttttc ggccagggtg atttatcccg ttccacctca
     1321 atgacttctt gggtagtaaa ctcgaatttt ttattgggtt cgcttaagta tttaatgacc
     1381 cattctaaac gcgattccat acgctgtttg agggtgcgat aaatggcaaa gccattttct
     1441 aaattgcccg ctttaatatc gtcatcaaat tggtggcgga agcggttaaa gccgctgatg
     1501 tctgactgta aaaaatacat ccgcgaggca tcaaggcttt caaggtattt atcgagaaat
     1561 gcctctgaca gttgatcgtc aatggggagg ttgcggtagt gacgggtgga taacatcttg
     1621 acaacgtctt tgatggtcgc cgactgcacc tgggtcggct gtagttggct cacggtttct
     1681 gcgaaacagg agaggcttaa g
//
LOCUS       DAOQGS010000780         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_52290_length_1685_cov_4.241104, whole genome shotgun sequence.
ACCESSION   DAOQGS010000780 DAOQGS010000000
VERSION     DAOQGS010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52290_length_1685_cov_4.241104"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKZ52_18765"
     CDS             <1..928
                     /locus_tag="PKZ52_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HNG61869.1"
                     /translation="INTPPAVTAAPEAISAGQIRLEALPLKGLKTAGLALGVLAAMAL
                     AWPLMQWLTWAFNLQPLLGVGLLMVITSLFSACGFSLLQLIKGQAQVKQLGQLQEQAE
                     ALAGHKTFGKARPFVAALQGFYAGKPQGDMLAEVLKSLPDYADDRETLAHIERGFLAP
                     LDAEALRLINRYSSQTALAVAVSPWVSLDMALSLFRNAQMLSRIGELYGLRPSPLTRW
                     RLLKLVVAHLIGTGASEWALDQAVDTLGLGAAQMVGLRATQGIGAGLYTAKIGLAAMD
                     VCRPMGFVAEPKPVFKHLWEHLVAQVRGRFKI"
     gene            1446..>1685
                     /locus_tag="PKZ52_18770"
     CDS             1446..>1685
                     /locus_tag="PKZ52_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61870.1"
                     /translation="MLIRPAQKNHAYAIAKLHTTSWQTHYTDALSTHYLHHLAPAERE
                     ALWLERMANPKPNQQVFIAEEHNELVGFACVFLDEN"
BASE COUNT          380 a          469 c          449 g          387 t
ORIGIN      
        1 cataaatacc ccgcctgctg tgaccgccgc ccccgaggcc atcagcgccg ggcaaattcg
       61 cctggaggca ctgcccctga agggcctaaa aaccgccggc ctcgcccttg gggtactcgc
      121 cgccatggcc ctggcctggc cgctgatgca gtggctcacc tgggcgttta acctgcagcc
      181 gctgttgggg gtgggtttgt taatggtgat cacctccctg tttagcgcct gtggttttag
      241 cctcttgcaa ttaattaaag gccaggcgca agtgaaacaa ctgggccaat tacaggaaca
      301 agccgaggcc ttagcaggcc ataaaacctt cggcaaggcc cgcccatttg tggccgccct
      361 gcaggggttt tatgcaggca aaccccaggg cgacatgctc gccgaggtac ttaaaagcct
      421 gccggattac gccgacgata gggaaaccct cgcccatatt gagcggggtt ttttagcccc
      481 cttagatgct gaggccctgc ggctgatcaa ccgctacagc agccaaacgg ccctcgccgt
      541 agcggtaagc ccttgggtga gcctggatat ggccctgtcg ctgtttcgca acgcgcaaat
      601 gctctcgcgc atcggtgagc tctacggcct gcgcccaagc ccgctcaccc gctggcgatt
      661 actgaaattg gtggtggccc atttaatcgg cactggcgcc agcgaatggg ccctggacca
      721 agccgtcgac accctagggc tcggcgccgc ccaaatggtg gggctgcggg caacccaagg
      781 cataggcgcg ggtttatata ccgccaaaat tggcttggcg gcgatggatg tgtgcaggcc
      841 catggggttt gtggcggagc ctaaacctgt gtttaaacat ttgtgggagc atttggtggc
      901 gcaggttagg gggcggttta agatttaacg gtagaactaa tttttacctg tgcgattaaa
      961 ttatttattc agaagtgttt aattcaattc caggttgggc gccccatagc ctttaatcta
     1021 tgtattttgt gccgttattt atccaatcgc gcggttgccc ataaacccca ccccaaccct
     1081 ccccttaaaa aggggaggga gcaaaaccat gagccccagc ctaggcatca gtgtgaggtt
     1141 ttttgctcct cccccttttt aagggggagg ctgggtgggg gtttctggcg gagggtgcaa
     1201 tctcattaaa aaacgatgca atcgccttga gggaatggct ttgctcgtta aattagagag
     1261 gctaaatagg cagttctgcc ctttgtgggc cgggtattaa tgctaagcat tcttcatcca
     1321 ggctacgtcg gcaatttcag ccccgtaggc tgggcagtaa tgcccagcat ttgtaattaa
     1381 cccccggtaa taacggtcct tattttttaa cgtctaaaaa tccaaaataa ctcacgggat
     1441 caaaagtgtt aatccgcccc gcccaaaaaa accacgccta cgccatagcc aaattacaca
     1501 ccacaagctg gcaaacccat tacaccgacg ccctcagcac ccattattta caccacttag
     1561 cccccgccga gcgcgaagcg ctttggttgg agcgaatggc caatccaaag ccaaatcaac
     1621 aggtttttat tgccgaggaa cacaatgagt tagtgggctt tgcctgtgta tttctcgatg
     1681 aaaac
//
LOCUS       DAOQGS010000781         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_53023_length_1673_cov_3.006799, whole genome shotgun sequence.
ACCESSION   DAOQGS010000781 DAOQGS010000000
VERSION     DAOQGS010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53023_length_1673_cov_3.006799"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..940)
                     /locus_tag="PKZ52_18775"
     CDS             complement(98..940)
                     /locus_tag="PKZ52_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNG61871.1"
                     /translation="MSDDSPSSQPKSWLDRLIQTFSSNEPRSREELLDIIKEAANNKL
                     VDQEALNIIEGALDVADLQVREIMVPRSQMVVIKSEETAEEFLPKVIESGHSRFPVIG
                     ESLDDIKGVLLAKDLLPLILNGTQQFSLASILRPANIIPESKRLNILLKEFRENRYHM
                     ALVIDEFGSISGLLTIEDILEEIVGDIEDETDDDSDEDFIRQVSDRDYVLKALTAIED
                     FNEHFGTTFSDDEFDTLGGLLTQAFGHIPTRNETIELEGFTFRILYADSRQIHLIRLT
                     TPAI"
     gene            complement(971..1423)
                     /gene="ybeY"
                     /locus_tag="PKZ52_18780"
     CDS             complement(971..1423)
                     /gene="ybeY"
                     /locus_tag="PKZ52_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNG61872.1"
                     /translation="MENLDLQVASSAQDIPTLDDFQTWLALAIPGDQQHWDITIRVVD
                     EAESQSLNRDYRNKDYATNVLSFPADVPAELNIPLLGDLVICAPVVAREAAEQAKPAK
                     AHWAHLCIHGALHLLGYDHETEAEAEEMEALETKLIMGLGFADPYRAD"
     gene            complement(1425..>1673)
                     /locus_tag="PKZ52_18785"
     CDS             complement(1425..>1673)
                     /locus_tag="PKZ52_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNG61873.1"
                     /translation="TTAVITGDPTQIDLPRGTASGLRHALEVLDGVPGISFTRFSSKD
                     VVRHPIVQRIVDAYAAQEAREAAQKETAHKEDVRKGDA"
BASE COUNT          369 a          389 c          467 g          448 t
ORIGIN      
        1 cataggctgt tgagtcgaga aaacatagtg gggtttttaa tcagtaggtg cctggaaatc
       61 tgctggtggg ctgccgatca ctataaggtt aatttattta aatagcgggt gtagtcaggc
      121 ggattaagtg tatttgcctg ctgtcggcgt agagaatacg aaaggtaaaa ccctccaatt
      181 caatggtttc attgcgggtg gggatatggc caaaggcttg agtgagcagg cctcctaagg
      241 tatcaaattc gtcgtcgcta aacgtagtgc caaaatgctc gttaaagtct tcaatggcgg
      301 tgagtgcctt gagcacatag tcgcggtcgg acacttggcg aataaaatcc tcatcgctgt
      361 catcatcggt ttcatcttca atatcgccga caatttcttc caaaatatcc tcaatggtaa
      421 gcaggccaga aattgagcca aattcatcga ttaccagcgc catgtggtag cggttttcgc
      481 gaaattcttt taatagaata ttcagccgtt tgctttctgg gatgatattg gcggggcgta
      541 aaattgaggc cagggagaat tgttgggtgc cgttgagaat tagcggtaat aggtctttgg
      601 ctaaaagcac acctttgatg tcatcgaggg actcgccaat gaccgggaag cgggagtgac
      661 cactttcgat taccttgggt aaaaattctt cggcggtttc ttcgcttttg attaccacca
      721 tttggctgcg gggcaccatg atttcccgca cttgtaaatc ggccacatcc agtgcgcctt
      781 caataatatt taatgcctct tgatccacca atttattgtt ggccgcctct ttaataatat
      841 ccagtaattc ctcccgcgag cggggttcat tgctggagaa ggtctgaata aggcgatcta
      901 accaggattt gggttgacta ctcggcgagt cgtcggacat aaaatttcct tcagtaacca
      961 ggtaattaag ttaatcggca cggtatgggt ctgcaaatcc taggcccatg atgagtttag
     1021 tttctaaggc ttccatttct tcggcctcag cctcggtttc atgatcgtag cccaataaat
     1081 gcaaagcacc atggatacac aggtgggccc aatgggcttt ggcgggtttg gcttgctcgg
     1141 ccgcctcgcg ggcaaccacg ggggcgcaga tcactaaatc gcccaagagg gggatgttca
     1201 gttcggcagg cacatccgct ggaaacgata acacgttggt tgcgtaatct ttattgcggt
     1261 aatcccggtt gaggctctgg ctttccgctt cgtccaccac ccggatggtg atatcccagt
     1321 gctgttgatc gccggggatg gcgagggcca gccaggtttg aaagtcgtct agggtgggta
     1381 tgtcttgggc gctgcttgcc acttgtaggt cgaggttctc caccttaggc gtcaccctta
     1441 cgcacatctt ctttgtgggc ggtttccttc tgtgccgcct cccgcgcctc ttgagcggcg
     1501 taggcatcca cgatgcgctg gactatgggg tggcgcacaa catctttaga gctaaagcgc
     1561 gtaaagctga taccgggcac gccatcgagt acttctaggg cgtggcgcag gcctgaggcg
     1621 gtgccgcggg gcaggtcaat ttgcgtgggg tcgccggtga tcacggcagt ggt
//
LOCUS       DAOQGS010000782         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_53067_length_1672_cov_4.478046, whole genome shotgun sequence.
ACCESSION   DAOQGS010000782 DAOQGS010000000
VERSION     DAOQGS010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53067_length_1672_cov_4.478046"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="xseA"
                     /locus_tag="PKZ52_18790"
     CDS             <1..879
                     /gene="xseA"
                     /locus_tag="PKZ52_18790"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNG61874.1"
                     /translation="QVLKKRMPYCAVLVVPVAVQGAGAAGQIAAAIARVNREALAEVI
                     ILGRGGGSLEDLWAFNEEVVARAIFASAIPIISAVGHETDVTISDFVADQRAPTPSAA
                     AAHAVPDSAELLSYVEGLALNLAQRTQQQIHSLQQRLHIACGRLQNPRRQLLLRQQHI
                     DLLQLRLQTSIRRQLHSCTQRRQQLQHRLIQQSPQNRLALQSQNRAALKARLARAMLA
                     LLAQKRQQMQQAAGLLNNLSPMPTLERGYSICLTQEGSLIRTLADIPPKPFRLRLRDG
                     ETWVTKAPKEAPEANT"
     gene            1087..>1672
                     /locus_tag="PKZ52_18795"
     CDS             1087..>1672
                     /locus_tag="PKZ52_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61875.1"
                     /translation="MQQLIATFDQFNVNKKLSVFFGLLLAVLLIQAGLSLALLNGVGS
                     HGLIAAKELAPQTESAILMEYLVAEAHRTTEETMSGVAGADKETAEFLATARWYLNAL
                     LKGGSRDGLDIIPTQNPAVIQKFEEVGRQINRFEQVGLQRMEHAKHKLSAGGDTDAKF
                     DESFERLITSLDDWQQRAGKALTPEAAVAIGDMKY"
BASE COUNT          403 a          520 c          455 g          294 t
ORIGIN      
        1 caggtgctga aaaaacgcat gccctattgc gcggtgctgg tggtgccagt ggccgtgcag
       61 ggagcgggcg ccgccgggca aattgccgcc gccatagcgc gggtgaatcg agaagccctg
      121 gcggaggtga tcattctcgg ccggggcggc ggcagccttg aagacctgtg ggcatttaat
      181 gaagaagtgg tagcgcgggc gattttcgca agcgcaattc ccatcattag cgccgtgggc
      241 catgaaacgg atgtgaccat cagcgatttt gtcgccgatc agcgcgcccc caccccgtct
      301 gccgccgcag cccacgcggt acccgacagt gccgagctct taagttatgt agaaggctta
      361 gccctcaacc tcgcccagcg cacgcagcaa caaatccact ccctgcaaca gcgcctacac
      421 atcgcctgcg gccgcctgca gaatccgcgc aggcagttgt tactgcgcca gcagcatatc
      481 gacctgcttc agctgcgcct gcagaccagc atccgccgac agctgcacag ctgcacccag
      541 cgccgccagc agctccagca ccggttaatc cagcagtcgc cgcaaaaccg cctggcttta
      601 caaagccaaa accgcgcggc gctgaaggcg cgactggcgc gggcgatgct ggcgctgctg
      661 gcgcaaaaac gccaacagat gcaacaggcc gcaggcctac tcaacaacct aagccccatg
      721 cccacactgg agcgcggcta cagcatctgc ctcacccaag agggcagttt gatccgcaca
      781 ctcgcggaca ttccccccaa accctttcga ctgcgcctgc gcgatggcga gacctgggtg
      841 accaaagcgc ccaaagaagc gcccgaggca aacacctaag ctgcaatcac caccccaacg
      901 ttagttacct aaaaacctgt aggggcgccc gcctcaccta aatcctagtc agtacccgag
      961 tttcacaggg caagggttgc cccctaccac tggtcgccat cagcaaacag gctttgatgg
     1021 gcgccgattc ttaggcttaa cttcatatac gcaagcaggc aacggattta aattcctggg
     1081 gacacaatgc aacagttaat cgccactttt gaccaattca acgtcaataa aaaactgtcc
     1141 gtatttttcg gcttactcct agctgtgttg ttaattcaag cgggcttaag ccttgcgcta
     1201 ttaaacggcg tcggcagcca cggcctcata gcggccaaag aattagcccc acagacggaa
     1261 agcgccatat taatggagta tttggtggcc gaggcccacc gcaccacgga ggagaccatg
     1321 agcggtgtgg ccggtgccga taaagaaacc gccgagtttt tagcgaccgc gcgctggtat
     1381 ttaaacgccc tgttaaaagg cggcagccgc gacggcctgg atattatccc cacccaaaac
     1441 cccgctgtaa tccaaaaatt cgaggaagtg gggcgacaga tcaaccgctt tgagcaggtg
     1501 gggctgcagc gcatggaaca cgccaaacat aaactcagcg ctggcggcga tacagatgca
     1561 aaatttgacg aaagttttga gcgcttaatt accagcttgg acgattggca gcagcgtgcg
     1621 ggcaaagccc tgacacccga ggccgcagtg gccatcggcg atatgaagta tt
//
LOCUS       DAOQGS010000783         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_53119_length_1671_cov_4.631807, whole genome shotgun sequence.
ACCESSION   DAOQGS010000783 DAOQGS010000000
VERSION     DAOQGS010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53119_length_1671_cov_4.631807"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..735)
                     /gene="nfuA"
                     /locus_tag="PKZ52_18800"
     CDS             complement(130..735)
                     /gene="nfuA"
                     /locus_tag="PKZ52_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HNG61876.1"
                     /translation="MSELSQSDLGVSISESAQEYLAGLLAKQDCEGIAIRMFVSSPGT
                     PQAETCIAYCRPGEEKEGDVKLQLTGLVAWFEGRSLPYLVDAKVDYSPDRMGGQLTIR
                     APNSKMPKISDDSPIEDKINYVLFNEINPGLASHNGNVSLVEVTEDKYAVLKFGGGCQ
                     GCSAVDVTLKEGVEKTLVSKIPELAGVKDVTDHSDTSKAYY"
     gene            864..>1671
                     /locus_tag="PKZ52_18805"
     CDS             864..>1671
                     /locus_tag="PKZ52_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007642244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNG61877.1"
                     /translation="MPHTARTQALHQQLAQRILILDGAMGTMIQSYKLKEADYRGERF
                     KDFHTDVAGNNDLLCLTQPQIIRDIQEAYLAAGADILETNTFNSTQVSQADYDMQPLA
                     RELNVAAARLSREACDKFTALTPDKPRFVAGVLGPTSRTCSISPDVNDPGARNITFDE
                     LVENYTEATEGLIEGGADIIMIETIFDTLNAKAAIFAVKKVFSQQGYELPIMISGTIT
                     DASGRTLSGQTTEAFYNSLQHAKPISIGLNCALGAQELRPYIKELSRVASC"
BASE COUNT          448 a          464 c          360 g          399 t
ORIGIN      
        1 attcagcgtc ttaggcaccc tcgctacgag ttcaactgcg gtttttaggt tcaaggtttt
       61 taggttaaag aatcacgcga actaaaatgc gttaggaaaa tacaccctaa acacgctggc
      121 gggggaaact taataatagg cttttgaagt atccgaatga tccgtcacat ccttaacgcc
      181 tgccaattca gggattttag acaccagggt tttctccacc ccttctttta aggtgacatc
      241 cacagcactg catccctggc agccgccgcc aaacttcaac acggcgtatt tatcttcagt
      301 aacttccacc agcgacacat tgccattgtg ggaggcaagg cctgggttaa tttcgttaaa
      361 taaaacgtaa ttaattttgt cttctatggg gctgtcgtca gaaattttgg gcattttaga
      421 attgggcgcg cggatggtga gctgcccgcc catgcgatcg ggggaataat ccaccttggc
      481 atccaccaaa taaggcaggc tgcggccttc aaaccaggca accaggccgg tcagttgcag
      541 cttcacatca ccttcttttt cttcaccggg gcggcaatag gcaatacagg tttccgcctg
      601 gggggtgccg ggtgaggaga caaacatacg aatggcgatg ccttcacaat cttgcttagc
      661 cagcaacccg gctagatatt cctgggcgct ctcactaatg gaaacaccca aatcactctg
      721 acttaactca ctcatatggc ccccaatcta tacccgataa aaacagtcaa gtattctaac
      781 ccagtccaac ctgggttgaa accgtggaca gaatctatct gatacaatac acctaaatca
      841 aaaaattttg atataggtta cgcatgcccc acaccgcccg cacccaagcc ctccaccagc
      901 aacttgccca acgcatcctg atcctcgacg gcgccatggg caccatgatt caaagttata
      961 aacttaaaga agcggattat cgcggagagc gctttaagga ttttcatacg gatgtagcgg
     1021 gcaacaacga tttattgtgc ttaacccagc cgcagattat tcgcgacatt caagaggcct
     1081 atttagccgc cggggccgat attcttgaaa ccaatacctt taatagcacc caagtcagcc
     1141 aagccgatta cgacatgcag cccctagcgc gggaattaaa tgttgcggca gcccgtttaa
     1201 gccgggaagc ctgcgataaa ttcaccgccc tcacccccga caaaccccgt tttgtagccg
     1261 gcgtattagg ccctacctcc cgcacctgct cgatttcacc cgatgtaaac gacccaggcg
     1321 cgcgcaatat tacctttgat gaattggtag aaaactacac cgaagccact gaaggattaa
     1381 ttgagggtgg cgcggacatc attatgattg aaaccatttt cgataccctc aacgccaaag
     1441 ccgctatttt tgccgtaaaa aaagtatttt cgcagcaagg ctatgagctg cccattatga
     1501 tctctggcac catcactgac gcctctggcc gcacgctttc cggccaaacc acggaagcgt
     1561 tttataattc cttacaacac gccaaaccta tttccatcgg cctaaattgc gccctaggcg
     1621 ctcaggaatt gcgtccctac ataaaagaat tatcacgggt tgccagctgt t
//
LOCUS       DAOQGS010000784         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_53122_length_1671_cov_4.443069, whole genome shotgun sequence.
ACCESSION   DAOQGS010000784 DAOQGS010000000
VERSION     DAOQGS010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53122_length_1671_cov_4.443069"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1008)
                     /locus_tag="PKZ52_18810"
     CDS             complement(217..1008)
                     /locus_tag="PKZ52_18810"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNG61878.1"
                     /translation="MTAIAIIGGSGVYGLPDLCPQELTRQSTPYGEVTGILKGTWPLS
                     TDTAIKPSANQTAPLNLYFLPRHGKSHALAPHKINYRANIWALKSLGVNKILALNAVG
                     SLFSPPGTWLLPEQIIDYTYGREHSFFDDFSQGVTHTEFTEPLHLSLINNLKHALADS
                     ELCLVQGGVYGCTQGPRLETAAEINRLERDGCSMVGMTLMPEAALAAELSIAYASLCL
                     SVNWAAGKCSAAITLEEILQCLQQEHSRLSQYWPDIINALAATQI"
     gene            complement(1269..>1671)
                     /locus_tag="PKZ52_18815"
     CDS             complement(1269..>1671)
                     /locus_tag="PKZ52_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61879.1"
                     /translation="ITPAFMAEIVATISQLARQSSHINQRSGVSVRLTVTNAETLVAN
                     AARRALVLGKYPEAKVSTVQGMGSSAPLNPTDPAAAINRRIAIIALKKAVSDALNNGT
                     SGISSQDLMSAEQPKTLIPEDPPPATYQRLK"
BASE COUNT          377 a          376 c          473 g          445 t
ORIGIN      
        1 tttacgtgag ttgccaataa tttataggac gggctgatta agtttgaggc gggggtattg
       61 agcaagcgtg gtgggcaggg atcgtgcgga gcacttatac ctttttacaa aattacccaa
      121 ttcagatttg atctgagcat tttacagcta gttagtcaat taattttttt agcgcactat
      181 tttctgtgcg ctttatcgat ggcgggcaat aggttgttat atttgcgtgg ccgctaaagc
      241 gttaataatg tccggccaat attggcttaa acgactgtgc tcttgctgca aacactgcag
      301 tatttcctca agcgtaatgg ctgcactgca tttacctgcc gcccaattaa cacttaggca
      361 taagctggca taagcgatgg aaagctctgc cgccagtgct gcctctggca ttaatgtcat
      421 gcctaccata ctgcagccat cgcgctccag gcgattaatt tccgctgcgg tttcaagccg
      481 cgggccttgg gtgcagccat acacgccacc ttgcactagg cataattcgc tatcggccaa
      541 ggcgtgttta aggttattaa ttagcgacag atgtaatggt tccgtaaatt ccgtgtgtgt
      601 aaccccttgg ctaaaatcat caaaaaagct gtgctcccga ccgtaggtat aatcaataat
      661 ttgctcgggt aatagccaag tgcccggcgg agaaaataaa ctgcccaccg cattgagcgc
      721 gagaattttg tttaccccta aggatttaag cgcccaaata ttggctcggt aattaatttt
      781 atggggcgca agtgcgtggc ttttgccgtg gcggggcaaa aaatacaggt ttagaggtgc
      841 tgtttgattg gcggatggct taatggcggt gtcggtgctt aatggccaag tgccctttaa
      901 aatgcctgtc acttcgccat aaggggtaga ttggcgagta agttcctggg ggcataggtc
      961 tggcaagccg taaacacctg agccaccgat aatcgcaatt gccgtcataa aataccttgt
     1021 gtgttactca gtgccgcagg gcagttaatt tatgcagctg cagagcattc tgatgggcat
     1081 aaatcttctg cgggcacaaa aaattcaaaa cgggcgcgcc ccgtacattt gcgattcgcg
     1141 cctcgcaggt tttcaagtaa gcgcatttta cccatgtcag ccgtttacgg gggagaaatt
     1201 ttggcgggca cagcgtatct gcctctgggc gctccaaagt taagcggata gaagggttcg
     1261 gctgagtttt atttgaggcg ctggtaagtg gccggtggtg ggtcttcagg aataagcgtt
     1321 ttgggttgtt cggcgctcat taagtcctgg ctggaaattc cgctggtgcc attattgagt
     1381 gcgtcagaaa cggctttttt aagggcgata atagcgatgc gacggttaat ggcggcggcg
     1441 ggatcggtgg gatttagcgg tgctgacgaa cccataccct gtaccgtaga aacttttgct
     1501 tccggatatt tacctaatac cagtgcgcgg cgggccgcgt tggcgaccag ggtctcggcg
     1561 ttggtgaccg tgaggcgcac cgacacgccc gagcgctggt tgatgtggct gctctgccgg
     1621 gcgagctggg agatggtggc cacgatctcg gccatgaacg ccggggtgat c
//
LOCUS       DAOQGS010000785         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_53458_length_1665_cov_4.645963, whole genome shotgun sequence.
ACCESSION   DAOQGS010000785 DAOQGS010000000
VERSION     DAOQGS010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53458_length_1665_cov_4.645963"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1251)
                     /locus_tag="PKZ52_18820"
     CDS             complement(25..1251)
                     /locus_tag="PKZ52_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61880.1"
                     /translation="MAVNTQYPGYDEVFRSAGAGRTVYARSNQAKELKKMLKIQRVNY
                     QYTLLGQGVAILSSDPSRATTLNAAGDMRRLTVGGGVSLTFYIYNGAIFIESIALGAL
                     VKTAPIGIYKVEYKEAQNFWVADTQSVNSFDSTQLWRSKKDKAHYAVIGDKFGLDIGN
                     AGRLLADHVLGGYSKEQYFLRLDITDRETPHFSLLWLDKEKHKSAQAVNLLSTAIIDS
                     YNKGLPVNWLVHGQGVHTFKKAVSQIKSSISAAALTVNDGVSGIKQQVYFSNPVSGSK
                     DSLKKLCESSGFAFVGYNANERDLKRLGNAINVGMEMGKQALMTAAGGSSIAGVGFGL
                     KQLGLGGIEKAMQNGADALVNGNHLTAAVAAMGCGFIVWGAVKKWQAIGAGIKCTFGK
                     GNQYWYSGDKELLAAQ"
     gene            complement(1278..>1665)
                     /locus_tag="PKZ52_18825"
     CDS             complement(1278..>1665)
                     /locus_tag="PKZ52_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61881.1"
                     /translation="EWIYCETWRQRFEEKNLRDMHTPDFDSFFIASMFTPLFSDKCLY
                     FSAWSMSGYPQGPLNRLKFERIDAIANTFNLHLTPQALQEQKEVLAANPNDHYSVSRK
                     PEIWEYPSNAPDSNKPLVFTPPPSLF"
BASE COUNT          458 a          414 c          332 g          461 t
ORIGIN      
        1 gattcgagag gtgtcacttc aattttattg agccgccaat aattctttat cgccggaata
       61 ccaatactgg tttccttttc caaaggtgca tttgattccc gctccaatgg cttgccactt
      121 tttgacggcg ccccaaacga taaatccgca gcccatagcc gccacggcag ccgtcaaatg
      181 gttgccattc acaagcgcat ccgccccgtt ttgcatggct ttttcaatgc cgcctaaacc
      241 taactgcttc agcccaaatc caacgcctgc aatactgcta ccaccagcag cggtcattaa
      301 ggcctgtttg cccatttcca tacctacgtt gatggcgttg cctaatcgct ttaaatcgcg
      361 ctcattagcg ttataaccca cgaatgcaaa cccagagctt tcgcaaagtt tttttaacga
      421 atctttgctg ccactaactg gattggagaa atatacctgc tgcttaatgc cggacacacc
      481 gtcattaacc gttagtgctg ccgcgctgat actggattta atttggctga cggctttttt
      541 aaatgtgtgc acaccttggc catgcaccaa ccaattcacc ggtaaaccct tgttataact
      601 atcgattatg gcggtgctga gcaaattaac tgcctgagca gatttatgct tttctttatc
      661 tagccataat agtgaaaaat gaggtgtttc tcggtcagta atatccagcc taagaaaata
      721 ttgctctttg ctgtaacccc ccagaacatg gtcagcgagc aaacgaccag cattaccaat
      781 atccaagccg aatttatcgc cgataaccgc ataatgcgcc ttatcttttt tgctgcgcca
      841 gagctgcgtc gaatcaaagc tatttacaga ttgagtatcg gcgacccaaa aattctgtgc
      901 ttctttatac tcaactttat aaatgcctat cggggcggtt tttacaagtg cccctaaggc
      961 gatagattcg ataaatatgg caccgttata gatgtaaaac gtcaaagaaa caccgccacc
     1021 aactgttaat cgcctcatat cgccagcggc gtttagtgta gtggcacgcg atggatccga
     1081 acttagtatc gctacacctt gccccaaaag agtatattga taatttaccc gctgtatttt
     1141 aagcattttc ttcaactctt tcgcttgatt gctgcgcgca tagactgtgc gacccgcacc
     1201 agcagagcga aatacttcat cataaccggg gtattgtgtg ttaacggcca tataaaaatc
     1261 cttagtgaaa ttatggatta aaaaagactg ggaggagggg taaaaacgag cggtttattg
     1321 ctatcggggg cgttgctagg atattcccaa atttctggtt ttcggctaac gctatagtga
     1381 tcattcgggt tagccgccaa tacttccttt tgctcctgta aagcctgagg cgtcaagtgc
     1441 aaattaaatg tattggcaat ggcatcaatc cgctcaaatt taagcctatt taacggcccc
     1501 tgcgggtatc cagacatact ccacgcagaa aaatagagac acttatcaga aaacaaaggg
     1561 gtaaacatac tagcaataaa aaacgagtca aaatctggcg tatgcatatc gcgcaaattt
     1621 ttctcttcaa accgttgtcg ccaagtctca caataaatcc actcg
//
LOCUS       DAOQGS010000786         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_54301_length_1651_cov_4.284461, whole genome shotgun sequence.
ACCESSION   DAOQGS010000786 DAOQGS010000000
VERSION     DAOQGS010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54301_length_1651_cov_4.284461"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..725)
                     /locus_tag="PKZ52_18830"
     CDS             complement(108..725)
                     /locus_tag="PKZ52_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNG61882.1"
                     /translation="MSQDLFADNTPGEVINIAGAQLLFFPQVQLPIAHDEVLARLIAQ
                     VPWVQEDIIIYGKRHPQPRLIAWYGDEGMNYRYSGVTHYPLPWSSILLELKHKVEALC
                     GCSFNSALVNYYRHERDSMGMHSDDERELGPQPVIASLSLGGPRTLIFKPKRGGARFT
                     LALPPGSLLVMQGDTQKNWLHGINKETTPCAPRVNITFRRIIQAD"
     gene            910..1620
                     /locus_tag="PKZ52_18835"
     CDS             910..1620
                     /locus_tag="PKZ52_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNG61883.1"
                     /translation="MAHYLIIGGTRGIGAAIVQHLLALDHHVSIWARNPPAAPLGERA
                     QFFYNNPAEQIPNITGLPEVLDGLVYCPGSIHLKPFARTSIEDFNQDFAVNVLGAVRS
                     LQAVTPLLKKSPQASVVLFSSVAAAVGMAFHASIASSKAAIEGLVRSLACEWAPAIRV
                     NGIAPSLTHTSLTEKLLNTAEKITAAEKRHPLQKIATAEEIAALAVFLLSPQAGFITG
                     QIMAADGGLSRLINPAAK"
BASE COUNT          429 a          449 c          384 g          389 t
ORIGIN      
        1 gctgccctaa aacaagtaaa tccacgcatt ttcgccagtt ttagggcagc gaaggccaaa
       61 tagatcggga cgatctattc agccgccggg ttaataaaca cgaaggatta atcagcttga
      121 ataatgcgcc taaaggtaat attcaccctc ggcgcacagg gtgtagtttc cttgttaatt
      181 ccatgcaacc aatttttctg tgtatcccct tgcatcacca acaggctgcc agggggcaag
      241 gctagtgtaa aacgcgcacc gccccgttta ggcttaaaaa taagggtgcg cggcccacct
      301 aagcttaaag aggcaataac cggctgcggc cccaactccc gctcatcgtc gctgtgcatg
      361 cccatgctat cgcgctcgtg gcgataataa ttgaccaaag cactgttaaa agaacaaccg
      421 caaagcgcct caaccttgtg tttaagttcc agtaatatgc tggaccaagg tagcgggtaa
      481 tgggtaaccc cggaataacg gtaattcata ccctcatccc cataccaggc aattagcctc
      541 ggttgggggt ggcgtttacc gtaaataata atatcttcct gcacccaggg tacctgtgca
      601 attaaacgcg ctaacacctc atcatgggca atgggcagct gcacctgcgg aaaaaataat
      661 aactgcgccc cagcaatgtt aattacctca ccgggcgtgt tatcggcaaa taaatcctgg
      721 ctcatagtta ttaccaaagg gcattttttg ggggcagttt tttacagccc cgcgtaatac
      781 taccttgcca atccaccacc gcatacctat tgcaacaggc tgacaacaaa cgcccgcaac
      841 aatatacgct cctgccatcc gcctgttatt aagatgcgta caaagagctt ccacttaagg
      901 cacttgttta tggctcatta tttaattatc ggcggcacac ggggcatagg cgccgccata
      961 gtgcagcatt tactagctct ggatcaccac gtgagtattt gggcgcgcaa cccaccagca
     1021 gcccccttag gtgagagggc gcagtttttt tataataacc ctgccgaaca aatcccaaat
     1081 ataacaggcc tgcccgaggt gctggacgga ttggtgtatt gccccggcag cattcattta
     1141 aaaccctttg cccgcacaag cattgaggat tttaaccagg attttgccgt taatgtttta
     1201 ggcgctgtgc gttcattaca ggcggtaacg cccttattaa aaaaatcccc ccaggccagt
     1261 gtggtgttat ttagcagcgt ggcagccgcc gtcggcatgg cctttcacgc cagtattgcc
     1321 agtagcaaag ccgccattga agggcttgtg cgcagcctcg cctgtgaatg ggcgcccgcc
     1381 attcgcgtta atggcatagc gccatccctc actcatacta gtctcacgga aaaactttta
     1441 aacaccgcag aaaaaatcac cgctgccgaa aaacgccacc cgctgcaaaa aatagccacc
     1501 gcagaggaaa tagcggccct ggcagtattt ttattatcgc cccaggcggg gtttattact
     1561 gggcagataa tggcggcgga tgggggttta tcgcggctta ttaacccggc ggcaaaatag
     1621 atcgtcctga tctattttgc cttcgctgca c
//
LOCUS       DAOQGS010000787         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_54472_length_1648_cov_4.527307, whole genome shotgun sequence.
ACCESSION   DAOQGS010000787 DAOQGS010000000
VERSION     DAOQGS010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54472_length_1648_cov_4.527307"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..530)
                     /gene="moaB"
                     /locus_tag="PKZ52_18840"
     CDS             complement(39..530)
                     /gene="moaB"
                     /locus_tag="PKZ52_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNG61884.1"
                     /translation="MPDTQKPLSFSVLTVSDTRSLDTDTSGQTLVELAEGAGHQLLER
                     CLVKDCIYQIRAVLSRWIADPQTQVVLITGGTGFSGRDSTPEAVAVLLDKQIPGFGEL
                     FRAQSAAEIGTSTLQSRALAGLANGTVVFCLPGSTGACRTAWQIIGPQLDNRTKPCNL
                     SRC"
     gene            complement(1108..1413)
                     /locus_tag="PKZ52_18845"
     CDS             complement(1108..1413)
                     /locus_tag="PKZ52_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61885.1"
                     /translation="MVPAELQILTSNTVIIVVAYFVVYPRFCGANLHKILFNDVIATL
                     VALLVAGAKFAGGTEVFSLGVGAVNWFWFTLLSYFLLETPFMIWYFKRHGIWPGAGK"
BASE COUNT          438 a          441 c          419 g          350 t
ORIGIN      
        1 gggttgcttg ggcctgcgcc aggcttaaca tgagggcttt aacaccgcga caaattacag
       61 ggcttggtgc ggttatcgag ctgggggccg ataatctgcc aggccgtgcg gcaggcgcca
      121 gtggagccgg gcaggcaaaa caccaccgtg ccgttggcaa gccccgcgag tgcgcggctt
      181 tgcagggtgg aagtgccaat ttccgctgcg ctctgggcgc ggaataactc gccaaacccg
      241 gggatctgtt tatccagcaa taccgccacc gcctcaggag tggaatcccg cccgctaaaa
      301 cccgtgccgc cggtaatcaa taccacttgg gtttgcgggt cggcgatcca gcggcttaac
      361 acggcgcgaa tctggtagat acagtctttc accaaacagc gctccaacaa ctggtgcccc
      421 gccccttcgg caagctccac cagggtttgc ccggaggtat cggtatccag gctacgggta
      481 tcagacacgg taagaacact aaacgacagg ggtttttggg tatcgggcat aggggcctca
      541 aggctcggcg cgcttataaa aagtgggcgg aagtttacac cctagtgtta agcgcccata
      601 gccgcaagca gtttaggggc cggtttatgt gggttggggc tgctgtttat tgttttcccg
      661 gggcgggggt gaagcgaaga catataacta gttgcaaccc aggcacggcc taaaattccc
      721 ctcccctgga ggggtgcccc gaaggggtgg ggtggtaaat cgctgcgctg ttttttgttc
      781 acagcccgct gtaattaaag atttcccgct ccctgaaata aaagtcagct gaaataaaag
      841 aaaactcacc tgcaataaaa cagggttctc caccgcagcg cctccaaggc aatgtcacta
      901 agttaataaa ttgggctgtt atttggcgta agtaatatct gggtagtctg gatggagccc
      961 agcgaaatcc aggagttttg aggcaccccg gattgcgcta ggctccatcc aggctacaaa
     1021 attggctggc aatccttaac ttaaccccag gcaggatcat ttttttgaag cttctttctc
     1081 gccagaaatt ttgctgtaag caaaaaatta tttacccgcc cccggccaaa tcccgtggcg
     1141 cttgaaatac caaatcataa aaggcgtttc cagcaaaaaa taactcaaca aagtaaacca
     1201 aaaccaattc accgcaccca cacccaacga aaacacctca gtacccccgg caaatttagc
     1261 ccccgccacc agcagggcaa ccagcgtggc gataacatcg ttgaataaaa ttttgtgcag
     1321 attcgcaccg caaaaacgcg gatacaccac aaaataggcc accacaataa tcactgtatt
     1381 actagttaaa atctgcagtt cagcaggcac cataaataat actcatacct agttttaatc
     1441 tgcttgatag gcaataaccc gcagggcaat ttttggcgcg gacatcacac ctgtagcgct
     1501 ctcgcgggcc tcctgccgta atcagagatt cctatataaa attaacccgg ccccagcagt
     1561 aaggaacctc tgaaaaatgt aggcggcgaa gcgctggcta ggcgaatttg gcaaaaaaag
     1621 cggagtttac acgcagtaaa tgagcatt
//
LOCUS       DAOQGS010000788         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_54835_length_1642_cov_5.464398, whole genome shotgun sequence.
ACCESSION   DAOQGS010000788 DAOQGS010000000
VERSION     DAOQGS010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54835_length_1642_cov_5.464398"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            807..>1642
                     /locus_tag="PKZ52_18850"
     CDS             807..>1642
                     /locus_tag="PKZ52_18850"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HNG61886.1"
                     /translation="MSKSLLCKSSLALGAALLLPSSNLHAIDNGTQTSNKFRINYAGY
                     LPQANKLALYISANTGAKSWSISGTNCSGTTSTYVSNDKSSGDSFYKIDFSSCVQTGT
                     NLKLVVGSDQSPAFDISSDPYGAMKYEFFDYFKDHETSATFNQTKNNWQSGLSLTFSY
                     VKDAGDNGAYPVNTAEAAWALMNMLETYPAINTYYSTRMSGARTMYQQLNTLTDQFNY
                     VLSHNRKLAIPKFHTNVNTSWAACSPHSSGTCISEPETKATFSTARALAGMARIHATY
                     GT"
BASE COUNT          425 a          423 c          364 g          430 t
ORIGIN      
        1 ggtttaacct cgtggtatcg aggaaatata acgtcgaaag gcgctgcgct tttctgactt
       61 accatctagg tggtgaatag gtttccaacg gtttttctgg tttaagcggg tgaccttctc
      121 gataactcgt ctacatcatc gaaaactcgg cacgaagatt tcggcagaat taacgagagc
      181 tagcgatttt aagacttaat gcgttgctca agcatcgcac acttcctgtg ctgaacagtt
      241 accgctgaat aaatggatcc tgcggcgcat ttctaactca tggctggtta cttaatttaa
      301 ataattacat taaagcccaa gaggcttgtc tattccgcgc gctcccaaag cgccgcccgg
      361 ttatctatta aacccgccca actccaaatt gccccgccag cctgaagagc caggttcgaa
      421 ccctggcgca aatactgctt tgaatgttgc ggtgaggtga agaccgcttt gtaagggctc
      481 tggctcaggc ggccacgatt gtgatgcaaa gcacagattg tgcttcacca cagcctggcg
      541 gcctggcagc cccattgctc agcccaataa aaacgcgcgc ggtggggctt gtggagatga
      601 tagcgatcac acttgaagat gattttggcg tcattggtta tttcttgtgc tagcatcttt
      661 aaacctaaac attcgcggag tatgtagttg ttggggttaa ggtttaacag actcagctct
      721 gttgcttacc cgggatccac taagcacatg ggcgtggttg cactgccacg gctttattga
      781 gttgtaattt ttattaggag atgtacgtgt caaaatcact attatgtaaa tcctctttag
      841 ccctgggcgc tgccctgctg ctgccaagtt ccaatcttca cgccatagac aacggcaccc
      901 aaaccagcaa taaattccgc attaactacg ccggttattt accccaggca aataaattag
      961 cgctgtatat tagcgccaat accggcgcaa aaagctggtc tattagcggc actaattgct
     1021 cgggcaccac cagcacttat gtaagtaatg ataaaagctc cggcgatagt ttttataaaa
     1081 ttgatttttc ctcttgtgtg caaaccggca ccaatttaaa gctggtggtg ggcagcgacc
     1141 aatcccccgc gtttgatatt tccagcgacc cttatggcgc tatgaaatac gaattttttg
     1201 attattttaa agaccacgaa accagcgcca cctttaacca aaccaaaaac aattggcaaa
     1261 gcggtttatc gctgaccttt tcgtatgtga aagatgccgg tgataacggt gcttacccgg
     1321 ttaataccgc tgaggccgcc tgggccttga tgaatatgct ggaaacctac ccagctatta
     1381 atacctacta cagcacgcgc atgagcggcg cgcgtactat gtaccaacaa ttaaataccc
     1441 ttaccgacca atttaattat gtgttatccc acaaccgcaa attggcgatt cctaaattcc
     1501 acaccaatgt aaatacctcc tgggctgctt gttcacccca cagcagcggt acctgtattt
     1561 ccgagccgga aaccaaagcc accttttcca ccgcgcgcgc tttagccgga atggcacgca
     1621 ttcacgccac ctatggcacc ag
//
LOCUS       DAOQGS010000789         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_54885_length_1641_cov_4.165826, whole genome shotgun sequence.
ACCESSION   DAOQGS010000789 DAOQGS010000000
VERSION     DAOQGS010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54885_length_1641_cov_4.165826"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKZ52_18855"
     CDS             <1..1047
                     /locus_tag="PKZ52_18855"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HNG61887.1"
                     /translation="RSLDTTRLIGQAFAHWNYDAGAEGTAGLEDFVGLNYNPGIYDSQ
                     HTKYPNRKFIGSETSSAVRSRGVYNNNNLQLSSYDNAIVGWGQTAENSWRDVNSREWI
                     AGEFIWTGFDYIGEPTPNEAWPAKSSYFGAIDTAYLPKDIFYFYQSRWNWSGPTMVHI
                     VPMNWTNWNAGQAVKVMVYSNADSVELFLNGTSLGSKTIAQTQGHIEWSVPFAKGTLE
                     ARARKGGRVVVTDTLRTANAAAQLGLLVDRATINADGSDLAFVEVDVVDAQGTLVPNA
                     NNLINFTLEGPGILVGVDNGNAVSLESYKGKSRSAFSGKAMAIVQSTRTPGTITLKAS
                     ASGLTPSEIKIKTE"
BASE COUNT          436 a          414 c          383 g          408 t
ORIGIN      
        1 cgcagtttgg atactacccg cttaatcggc caggcttttg ctcattggaa ttatgatgca
       61 ggcgctgaag gcaccgcagg ccttgaggat tttgtgggct taaattacaa ccctggtatt
      121 tatgattccc aacatactaa ataccccaac cgcaaattta tcggcagcga aacctcttct
      181 gccgtgcgca gccggggagt ttataacaac aataatctgc aattatcttc ctacgataat
      241 gccatagttg gttggggcca aacggcggaa aactcctggc gcgatgtgaa tagccgcgaa
      301 tggattgccg gtgaatttat ttggacgggt tttgattaca taggcgagcc cacaccgaac
      361 gaagcctggc ctgctaaaag ctcctatttc ggcgcaattg atacggccta tttacccaag
      421 gatatatttt atttttacca aagccgctgg aattggagcg ggccaaccat ggtgcatata
      481 gtcccaatga attggactaa ctggaacgcc gggcaagcgg ttaaagtgat ggtgtatagc
      541 aacgctgata gcgtggagct gtttttaaat ggcacttctc taggctcaaa aaccattgct
      601 caaacccaag gccatatcga gtggtcggtg ccttttgcta aaggcacttt ggaggctcgc
      661 gcgcgcaagg gcgggcgggt ggtggtcacc gatacactgc gcacagctaa cgcagcggct
      721 caattgggtt tattagtgga tcgcgcaacc attaatgccg atggcagtga tttagcgttt
      781 gtggaagtgg acgtagtaga tgcccaggga acattggttc ccaatgccaa taatctcatt
      841 aatttcaccc tcgaaggccc cggtatatta gtcggcgtgg ataatggcaa tgccgtaagc
      901 cttgaatcct acaaaggaaa atcccgctca gcctttagcg gcaaagccat ggctatagtg
      961 caatccactc gcacccccgg cactattaca cttaaagcca gtgccagcgg gcttacgccc
     1021 agtgaaataa aaattaaaac tgagtgaaat aaaaattaac agcgcgcagt aaaaattatt
     1081 aacccggcaa aaaaccgccg ggttaatccc ccatcccgtg caacccccgc cgggtcacat
     1141 gtcacctttc aaaggcaata cccttcttaa ggatcttaac ctgtttttcc tggtgactta
     1201 gccctttgcg aaaagcccgt tctggcgtag gttggaagag cgcagcgact tccgacatcc
     1261 cgcagagtcc ttgctgtacc tatagtttgt actggcggat gggtgtcaca agtcgctacg
     1321 ctcttccaat ctaagggtaa atattcacct caaaactcct aacttaatga cattgccacc
     1381 cagcctcccc ttgaaaaggg ggcggcgcta aaaagcctca aacgaagtaa ttttgctttt
     1441 ttcaaagctg ttttacgccc tccccatgtt ttctggggga gcgaaaacag ctggaaaata
     1501 gcacccaatc tttaacctag tgacacgtac tcagtgtgga ttctccttaa ggaacctctg
     1561 aaaaattacc tcactcgccc tagccacgtt gaatttggca aaaaatgctc atttactgcg
     1621 tgtaaactcg gcaattgctc c
//
LOCUS       DAOQGS010000790         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_54954_length_1640_cov_3.897161, whole genome shotgun sequence.
ACCESSION   DAOQGS010000790 DAOQGS010000000
VERSION     DAOQGS010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54954_length_1640_cov_3.897161"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..621
                     /locus_tag="PKZ52_18860"
     CDS             232..621
                     /locus_tag="PKZ52_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNG61888.1"
                     /translation="MSFPKHISDNFVVLTPDKHAHIEPFNANLYGRLDAVYGGFVGHE
                     LIACHSFTQDWGSWEMHPHGDEVVVLLSGAVRFVLQTPGGDTAVLLSEQGHYVVVPKG
                     VWHTAAIISPAKLLFITPGQGTEHRPA"
     gene            771..1442
                     /locus_tag="PKZ52_18865"
     CDS             771..1442
                     /locus_tag="PKZ52_18865"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNG61889.1"
                     /translation="MMSKINIGKLKTLLVSLGVLTQVAVAEPVKQGQWESRSQVWIDG
                     KEVLQQLHAAGDEIIKNARARMPADQRAQFDQQMAAARKDAGSELECIGPKEASMTPE
                     AIAEQSIKAVHQPPWQCSISEKKIAADGFSYQYSCRTSAGGASDGTVSLSLHATSYRF
                     EVVGTGHAVNGETGAVLGSTKMPLRSLTQGHWVSDKCDASTEDHDMGAEDDAPPAPKT
                     KHPKP"
BASE COUNT          403 a          349 c          449 g          439 t
ORIGIN      
        1 ttgccaaatt caacgccgcc agggcgagta aggtaatttt tcagaggttc cttagtcatt
       61 gtattcttag gtttatctgt gggtgtgtgc aatatgcggc gaccaagtta tggttgtgct
      121 gataaaaata gtcataattg cgcgctttaa aatattcact catgtagatt aattcgtgca
      181 tctttaacag ttggttaagc cctttaacct taattaagag acttatcacc tatgtcattt
      241 cctaaacaca tcagcgataa ttttgtagtt ttgactcccg acaaacacgc gcatattgag
      301 ccttttaacg cgaatttata cggccgtttg gatgcggtat atggtgggtt tgtcggccat
      361 gagttgattg cctgccacag ctttacgcag gattggggca gttgggagat gcatccccat
      421 ggtgatgagg tagtcgtatt gctcagtggc gccgtgcgct ttgtactgca aacacccggc
      481 ggtgacacgg ctgtgttatt aagcgaacag gggcattatg tagtggtgcc caagggggtg
      541 tggcacacag ctgcgattat ttctccggcc aaacttttgt ttattacccc tgggcagggc
      601 actgagcatc ggcctgcata agcacatgtg taaagcctca actaggcgct attgcacaag
      661 ggtaatttag ccttgtcact gttttggtta acccattatt cttgtggatg ccgtgtttaa
      721 gggcgctgta aattaagtta ttggtgaatg ggcgatttag cgaggatcat atgatgagca
      781 aaataaatat aggcaaatta aaaacgctac tggtgtcatt gggcgtatta actcaagttg
      841 cggtggcgga gccggtaaaa caaggccaat gggagagccg ctcccaggtg tggattgacg
      901 gtaaagaagt gttgcaacag ctgcacgcgg cgggggatga aattattaaa aatgcccgcg
      961 cgcgtatgcc cgctgaccag cgcgctcagt tcgatcaaca aatggcagct gcccgcaaag
     1021 atgcaggcag cgagttggag tgtattggcc ccaaagaggc gtctatgacg ccggaggcaa
     1081 ttgccgaaca atcgattaaa gctgtgcatc agccgccctg gcagtgctct atcagcgaga
     1141 aaaaaattgc cgcagatgga tttagttacc agtacagctg ccgcaccagt gccggtggtg
     1201 cctcagatgg cacggtaagc ttaagcctgc acgcaacctc atatcgtttt gaagttgtgg
     1261 gtacagggca tgcggtaaat ggcgaaacag gcgcagtgct cggctctact aaaatgcctt
     1321 tgcgttcact cacccagggg cactgggtga gtgataaatg cgatgcatca acggaagatc
     1381 atgatatggg ggctgaggat gacgcgccgc cagcgcctaa aacgaaacac cccaaaccct
     1441 agccgccaga tttaattagc gcttactcat ttgagctgct caggggtatt tgctcaattg
     1501 gctgttgtgc aggcggatta gttttttcgg cggcgtattt ttgtttggcg tgtttatacc
     1561 actcgatact aaaccagagg cgggtatcct gcatgggtat ggttttttgt aggtctggat
     1621 tgttgatgga taaaattcgc
//
LOCUS       DAOQGS010000791         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_55095_length_1638_cov_3.684776, whole genome shotgun sequence.
ACCESSION   DAOQGS010000791 DAOQGS010000000
VERSION     DAOQGS010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55095_length_1638_cov_3.684776"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1021
                     /locus_tag="PKZ52_18870"
     CDS             47..1021
                     /locus_tag="PKZ52_18870"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNG61890.1"
                     /translation="MKTVANCVVVGLGNMGWRYDERSDGVLTHAKAINEHPQLQLIAA
                     VDPNAQACNEFKQAYPHVATFPSLQAAAAQYRFDWIILAVPTHLHFAAFNEALSLKPE
                     VIVCEKPLAPSAAQAAEMLRRAKAQGVLVFVNYIRPYEPHCKAMFKFIREGAWGGVEK
                     IIVRYGKGITNNASHFIHLLVTALGRPEHVRLINANDTARADPEPDFVLQFGSVQAVF
                     LRFDYQLYALSEMDIYLQQGAIFYRAMGQRIEYQQAEADEVFPSVKRLQLVRTEATQL
                     SYYQRHALAYYYKQWQSRVGEDPLTQSALEALQVVEQVQAQLDDISAP"
     gene            1039..>1638
                     /locus_tag="PKZ52_18875"
     CDS             1039..>1638
                     /locus_tag="PKZ52_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNG61891.1"
                     /translation="MQTLALFGGSAAFTRQLVPYNPYGEEEVTAATEVIRSGCLSSFI
                     GAWGEGFLGGPQVRGFEAEWAQFFGVKHAISVNSLTSGLICAVGALDIEPGDEIIVSP
                     WTMSASATCILVWNAIPVFADINPDTFNLDFQSVVANITPRTRAIIVPSIFGYGADLE
                     ALRGLANAQGIKIIEDAAQAPAVKYAGKWVGTWGDIGGFS"
BASE COUNT          401 a          343 c          458 g          436 t
ORIGIN      
        1 tatagcagcc gaagagccgt taaattgggc gcatgtagaa taggatgtga agacggtggc
       61 caattgtgtt gtggtgggtt tgggtaatat ggggtggcgc tacgacgagc gcagcgacgg
      121 cgttttaacc catgctaaag caattaatga acacccgcag ttacagttaa ttgcagcagt
      181 agacccaaac gcacaggcgt gcaatgaatt taaacaagcc tatcctcatg tggcaacatt
      241 tcctagctta caggcagcag cagcgcaata caggtttgac tggattatcc ttgccgtgcc
      301 gacgcatcta cattttgcgg catttaatga agccttaagt cttaagcctg aggttatcgt
      361 gtgtgaaaaa cccctcgcac cgagcgcagc gcaagctgca gaaatgctcc gccgcgctaa
      421 ggcgcagggt gttttagtat tcgtaaatta tatccgcccc tatgaacccc attgtaaggc
      481 tatgtttaag tttattcgcg agggagcctg gggtggggta gaaaaaatta ttgtgcgtta
      541 tggcaaaggc attactaata atgcctccca ttttattcat ttattagtga ccgctttggg
      601 gaggcccgaa catgtgcggc ttattaatgc taacgatact gcgcgtgccg acccagagcc
      661 ggactttgta ttgcaatttg gctcggtgca ggcagttttt ttacgctttg attaccagct
      721 gtatgcttta agtgaaatgg atatttacct gcagcagggg gctatttttt atcgggctat
      781 ggggcagcgg attgaatacc aacaagccga ggcggatgag gtattcccca gtgttaaacg
      841 cttacagctg gtgcgcacgg aggcgacgca actaagttat taccagcgcc acgccttggc
      901 ctattattat aaacagtggc aaagccgagt gggcgaagac cccttaaccc aatccgcctt
      961 ggaagcatta caggtagtgg agcaggtgca agcgcaatta gacgatatta gtgcgcctta
     1021 attaatcgag aactatttat gcaaacacta gcactgttcg gtggctcagc agcatttacg
     1081 cggcaattag taccctataa cccctatggg gaagaggagg taactgcggc gacagaagtg
     1141 attcgctccg gttgtttatc cagctttatt ggcgcctggg gtgagggttt tttgggcggg
     1201 ccgcaggtgc gagggtttga agctgagtgg gcgcaatttt ttggtgtcaa acacgctatt
     1261 agtgttaatt cattaacctc gggtttaatt tgtgcggtag gggcgcttga tatcgaaccg
     1321 ggggatgaaa ttattgtaag cccctggacc atgtctgcct cagcaacgtg tattttggtt
     1381 tggaatgcta ttcccgtatt tgccgatata aacccggata cgtttaattt ggattttcaa
     1441 agtgtggtgg ccaatattac gcccagaacg cgggcgatta tagtgccgag tatttttggg
     1501 tatggcgcgg atttagaagc tctgcggggc cttgccaatg cgcaagggat taaaataatt
     1561 gaagacgccg cccaggcgcc agcggttaaa tatgccggta aatgggtggg cacttggggg
     1621 gatattggcg gttttagt
//
LOCUS       DAOQGS010000792         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_55141_length_1637_cov_5.087231, whole genome shotgun sequence.
ACCESSION   DAOQGS010000792 DAOQGS010000000
VERSION     DAOQGS010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55141_length_1637_cov_5.087231"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1637)
                     /gene="rnr"
                     /locus_tag="PKZ52_18880"
     CDS             complement(<1..>1637)
                     /gene="rnr"
                     /locus_tag="PKZ52_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNG61892.1"
                     /translation="DIYLTNRQMRKVFDGDEVLVRKGQTDFRGRIEGAIIEVIASPAK
                     TLVGRLYSERGINFLRADNPRIFQDILVADDALGGAKTGQLVELEVVDHPFKNRFPSG
                     RVVEVLGEHLAPGVEIDVAIRSHNIPYLWPQDVAAEAAAISPQVQEADKQLRIDMRHL
                     PLVTIDGEDARDFDDAVFCEKKRGGGWRLYVAIADVAHYVAVGSPLDREAQLRGNSVY
                     FPDYVVPMLPEALSNGLCSLNPAVDRLCMICEMTISAAGKITGYVFYESLMHSKARLT
                     YTQVGEILKERGVKTSVIRQRFSSVVPHLDELANLYAVLRSARDERGAIDFDTTETRI
                     IFDAQRKIDKIVPVVRNDAHKLIEECMLAANVCAAKFLEELGVPALYRVHEPPKERKL
                     ELLKDFLAELGLSLRSYEEIKPADYQAIIQAIADRPDASLIQTVLLRSMNQAVYSPEN
                     KGHFGLAYQAYTHFTSPIRRYPDLLTHRAIRSLIRSERPSKRVRRHPDAATIAPAVTY
                     PYSVADIVTMGEQCSLTERRADEATRDEMLEKSALLNR"
BASE COUNT          423 a          448 c          395 g          371 t
ORIGIN      
        1 cggttcagca aggccgattt ttccagcatt tcgtcgcggg tagcctcatc ggcccgccgc
       61 tccgttaatg aacactgctc gcccatagtt actatgtcgg cgaccgaata gggataggtc
      121 actgcaggcg caatagtggc tgcatctggg tggcggcgta cacgcttgga aggacgctcc
      181 gagcgaatca aactgcggat ggcgcggtgc gtaagcaaat ccgggtagcg ccgaatgggc
      241 gaagtgaaat gggtataggc ttgataggca aggccaaaat ggcctttatt ttccgggctg
      301 taaaccgcct ggttcatgct gcgcaacaat acggtttgaa ttaatgacgc atccggcctg
      361 tctgcgatgg cctgaatgat tgcctgataa tcagccggtt taatctcttc gtaactgcgc
      421 agggataagc ccaattcggc aagaaaatct tttaataatt ctaatttgcg ctctttaggc
      481 ggctcatgca cacgataaag cgccggcaca cctaattcct ctaaaaattt ggccgcacac
      541 acattggcgg ccaacataca ttcttcaatt aatttgtggg catcgttgcg cactaccggc
      601 acaattttat caattttgcg ctgggcatcg aaaataatgc gggtttccgt ggtatcaaaa
      661 tcaatcgccc cgcgctcatc gcgcgcgcta cgcaagactg catataaatt ggccagctca
      721 tctaagtgag gcaccaccga agaaaaacgc tgacgaataa cactcgtttt tacgccgcgc
      781 tctttcaaaa tttcacccac ctgggtgtag gttaagcgcg ctttggaatg catgagcgat
      841 tcgtaaaata cataaccggt aattttaccg gccgcgctga tggtcatttc acagatcata
      901 cacaatctgt ccaccgcggg atttaacgaa cacaaaccat tggacagggc ttctggcagc
      961 atgggcacaa cataatcggg aaaatacacc gaattaccgc ggagctgcgc ctcgcgatcc
     1021 agcggtgaac ccaccgccac atagtgcgcc acatcggcga tagcaacgta taaacgccaa
     1081 ccgccaccgc gttttttctc gcaaaatacc gcgtcgtcaa aatcccgcgc atcttcacca
     1141 tcaatggtca ccaacggcaa atggcgcata tcaatgcgca attgtttatc cgcctcttgc
     1201 acctgggggc tgatggcagc cgcttcagcg gccacatcct gaggccacag gtagggaata
     1261 ttatgggagc gaatcgccac atcaatttcg accccaggcg ccaaatgctc acctaaaact
     1321 tccaccacac ggccggaggg aaagcggttt ttaaacgggt gatcgaccac ctctaattca
     1381 actaactggc cggttttggc gccgccgagg gcgtcgtcgg ccactaaaat atcttggaaa
     1441 atgcgcggat tatccgcccg caaaaaatta ataccgcgct cggaatacaa gcggcctact
     1501 agggttttag ctggagatgc aattacctca ataatggcgc cttcaatacg gccgcgaaaa
     1561 tcagtctggc ctttgcgcac cagaacctca tcgccatcaa atactttgcg catctgccgg
     1621 ttggttaaat aaatatc
//
LOCUS       DAOQGS010000793         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_55309_length_1634_cov_5.502217, whole genome shotgun sequence.
ACCESSION   DAOQGS010000793 DAOQGS010000000
VERSION     DAOQGS010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55309_length_1634_cov_5.502217"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKZ52_18885"
     CDS             complement(<1..334)
                     /locus_tag="PKZ52_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HNG61893.1"
                     /translation="MPQLKIIVENAADWTSFYPSEDVVLARDYLAQPVGKERYRVINL
                     CNQYGYLSTGYYCSLLAEAREHQVIPSVKHIRDLSRRIWLGVVNVDASKDIAAVAGVV
                     HYGFSLGGF"
     gene            441..1535
                     /locus_tag="PKZ52_18890"
     CDS             441..1535
                     /locus_tag="PKZ52_18890"
                     /inference="COORDINATES: protein motif:HMM:NF023242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase/peptidase C39
                     family protein"
                     /protein_id="HNG61894.1"
                     /translation="MTLTTPRLATLDDLPALVALEMACFDYDQLTKRSFRYFITKAQA
                     QLWVIGEPVEAYGLVLFHRGTCLARIYSLAVSPECRGKGYGRLLIERLEQASEEHGTL
                     FIRLEVSDSNTNAIRLYEAMHYQPIRRLPQYYEDGHDGIRMEKRLLRHLTKPPHLPHY
                     AQTTEFTCGPAALMMAMKYLRPQTNMTQLEEINLWREATTVFMTQGHGGTSPLGLALA
                     AKKRGFTCALWLSVTTAPFLASVRCEEKKQIIELIHADFLARLQTEGIPTHSFPNKIE
                     HLKQQLQDGWAIILLISTYRFNRSKEPHWIWLVHIDDDYVYINDPDVDSEHWQSSLDN
                     IYVPVPIKDFEQMMKYGAKPYRSAILLRTE"
BASE COUNT          458 a          443 c          354 g          379 t
ORIGIN      
        1 aaaagccacc taagctaaag ccataatgaa caacacccgc cactgcagca atatctttgg
       61 atgcatccac attcaccaca cccaaccaaa tgcgcctcga tagatcgcga atgtgcttta
      121 ctgaaggaat aacctgatgc tcgcgcgctt ctgccaacag ggagcaataa tagccggtgc
      181 ttaaatagcc gtattgattg cataaattaa ttaccctata acgctcttta cccaccggct
      241 gcgcaagata atcgcgggcc aatacaacat cctctgatgg gtaaaaactg gtccaatcag
      301 cggcattttc tacaataatt tttaattgag gcatgaaaga cttttgaact caagtaaggg
      361 ataatgggac ggcctaaggg cctgttataa acacgctgcc aagccagttt ggcaaattat
      421 tgacgtttag caagagacct atgaccctca cgacaccgcg gcttgcaacc ctcgacgatc
      481 taccggctct tgtagcactt gaaatggcct gttttgacta cgaccaactc actaaacgca
      541 gttttcgcta ttttattacc aaagcgcaag cgcaactatg ggttataggc gagcctgtag
      601 aagcctatgg gctggttttg ttccatcgcg gtacctgcct tgctcgcatc tattcactgg
      661 cggtatcgcc cgagtgtcgc ggcaaaggct atgggcgcct gctgatagag cggctggagc
      721 aggcgtccga agaacacggc actttattta ttcgcctgga agtgtccgac agcaacacca
      781 acgcgattcg tttatatgag gccatgcatt accagcccat tcgccgttta ccccagtatt
      841 atgaagacgg tcacgacggc atccgcatgg aaaaacgcct actacggcac ctaaccaaac
      901 ccccacacct accccactac gcacaaacca ccgagttcac ctgcgggccc gccgcactta
      961 tgatggcgat gaaatatctg cgaccgcaaa ccaatatgac gcaactggag gaaattaacc
     1021 tctggcgcga agcgacaacc gtgtttatga cccaagggca cggcggcact tcacccttag
     1081 gcttagcgct ggccgctaaa aagcgcggct ttacctgcgc gctttggctg tcggttacca
     1141 ccgctccctt ccttgccagc gttcgctgcg aagaaaaaaa acagattatt gagctaatcc
     1201 acgctgattt tttagcgcgc ctgcaaaccg aaggcattcc cacacacagt tttcccaaca
     1261 aaattgaaca tttaaaacaa cagctgcaag acggctgggc cattatttta ttaattagca
     1321 cctaccgctt taaccgcagc aaagaacccc attggatttg gcttgtacac atcgatgatg
     1381 actacgtgta tataaacgac ccggatgtag actccgaaca ttggcagagc agtctggata
     1441 acatttatgt tccagtgccc attaaagatt ttgaacaaat gatgaaatac ggcgccaagc
     1501 cctatcgtag tgcaattttg ctgcgcactg aataaaactt tacaacctcg cttagctatg
     1561 aagatagggt acattattaa cccggcggca ttttatgccc agctagcaag agtagccggg
     1621 aacaataatt gacc
//
LOCUS       DAOQGS010000794         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_55314_length_1634_cov_4.932236, whole genome shotgun sequence.
ACCESSION   DAOQGS010000794 DAOQGS010000000
VERSION     DAOQGS010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55314_length_1634_cov_4.932236"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1572
                     /locus_tag="PKZ52_18895"
     CDS             373..1572
                     /locus_tag="PKZ52_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNG61895.1"
                     /translation="MSNLGLSAPITPTVPPQLQSVPSAEDLTRAFEFFNETSSLLSNS
                     YRTLEGRVAQLTRELTEVSQAREEEHVKNHRLAERIQALLDFLPGGVIVLDERGYIVQ
                     SNPAAKSMLENPLDGFKWRDIISQCFAPRRDDGLEVSTHQGKRISIATASFDQTGQII
                     LLTDQTETRRLQQQVARYEKLSAMGKMVSALAHQIRTPLSAALLYSNHLCNEDLEHEK
                     RQAFAHKLLGRLQHMERQVRDMLLFVRNELPLNEVITLADLQKGLQEAMEVPLASSKS
                     FCIWINPEPGLHIRCNREALISAVLNLVNNALQAKPGGNLAMRLEPITSANGLWLRLC
                     VVDDGPGMSKEFIAQAKELFFTTKPQGTGLGLAVVQSVARAHGGRFHLQSTEGKGTQA
                     SLILPLV"
BASE COUNT          414 a          391 c          410 g          419 t
ORIGIN      
        1 cgcggcatcc ttgccgcagt tattaacccg gcataaaaag ccgccgggtt aataacctga
       61 actggctgct tctggctgtt ggattaatta aggcttttag gattatagcc aagccctgct
      121 tattggcact ttgaattgcc ggtagcgcgg cggctaacaa tgaaattgag attagtgcca
      181 gtgctgcaga tgtgatgaat gatgtgcaac tcatctattg gcgtagaacg cctgtgcagc
      241 cgctacgctg ccccaccttg cgaatacata ggactcgccg attcagactc tataagagca
      301 gccttaaaaa attctggtat ccgccttgca ttgccccctc tagtgtcatt tttttaacgc
      361 ttgagtggtt ctatgtccaa cttaggatta agtgcgccaa taacacctac ggtgcccccc
      421 cagctgcaat cggtacccag cgcggaagat ttgacgcggg catttgaatt ttttaatgaa
      481 acttcgtcct tattaagtaa ttcctaccgc actttggaag gccgggtggc gcaattgacc
      541 cgcgagttga ccgaggtctc ccaagcgcgg gaggaggaac atgtcaaaaa ccatcgcttg
      601 gcggaacgta tccaggcctt attagatttt ttacccggtg gcgtaattgt tttagatgag
      661 cgtggttata ttgtgcaaag caatcccgca gcgaaaagca tgttagaaaa cccgctggat
      721 ggatttaaat ggcgggatat tatcagccag tgttttgcgc cgcgccggga tgacggttta
      781 gaagtatcta cccaccaggg taaacgcatc agtatcgcca ctgcatcgtt tgatcaaacc
      841 gggcaaatta ttttactcac tgaccaaacc gaaacccgcc gtttacaaca acaggtggcg
      901 cgatatgaaa aactttccgc catgggcaaa atggtttcag ccctcgctca tcaaattcgc
      961 actccactct ccgccgcact gctgtactcc aatcatttgt gtaatgaaga tttggagcat
     1021 gaaaaacgcc aggccttcgc ccataaatta ttaggccgcc tgcagcatat ggagcggcaa
     1081 gtgcgcgata tgctgttatt tgtgcgcaat gaattaccgc tcaatgaagt aattaccctg
     1141 gcggatttac aaaaaggttt gcaggaggct atggaagtgc ctttagccag cagtaaaagc
     1201 ttttgcattt ggattaaccc agagcctggg ttacatattc gctgcaaccg cgaggcatta
     1261 atcagcgccg tattaaacct agtgaataac gccctacagg caaaacccgg cggtaatttg
     1321 gcgatgcgtt tagagcctat taccagcgcc aatgggcttt ggttgcgcct gtgtgtggtg
     1381 gacgatgggc cgggtatgtc gaaggaattt atcgcccagg caaaagaatt attttttacc
     1441 accaaacccc agggaacggg ccttggttta gcggtggtgc aaagcgttgc gcgcgcccac
     1501 ggcggccgtt ttcatttgca atcgacggaa ggcaagggca ctcaagcctc tttaattctt
     1561 cctttggttt aatcagcgag agagtgttat ggcaagttca aatcaagcgg cgagtatttt
     1621 agtagtagaa gacg
//
LOCUS       DAOQGS010000795         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_56022_length_1623_cov_4.063138, whole genome shotgun sequence.
ACCESSION   DAOQGS010000795 DAOQGS010000000
VERSION     DAOQGS010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56022_length_1623_cov_4.063138"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="ftsY"
                     /locus_tag="PKZ52_18900"
     CDS             <1..695
                     /gene="ftsY"
                     /locus_tag="PKZ52_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNG61896.1"
                     /translation="LQENLRNLLLPVEKPLAIPADKKPFVILVVGVNGAGKTTTIGKL
                     AHRFLSEGKSVMLAAGDTFRAAAVEQLQVWGERNKVPVIAQHTGADSASVIFDALNAA
                     RARQVDVLIADTAGRLHNKSHLMEELSKVKRVLAKIDPASPHETLLVLDAGTGQNAVN
                     QCDQFHQSIGVTGLALTKLDGTAKGGIIFALSQKHKIPVRYIGVGETLDDLQPFAARP
                     FVDALFGKQSAQ"
     gene            692..1357
                     /gene="ftsE"
                     /locus_tag="PKZ52_18905"
     CDS             692..1357
                     /gene="ftsE"
                     /locus_tag="PKZ52_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNG61897.1"
                     /translation="MIEFRQVSKRYGDGHEALLGVNFSIAPKEMVFLTGHSGAGKSTL
                     MKLILLMERATSGQVLVAEQNLAKLRSGQIPFLRRQIGVVFQNHQLLFDRSVFHNVAL
                     PLQVCGIDPKDSARRVRAALDKVGLLEREKANPIALSGGEQQRVAIARALVHKPKILL
                     ADEPTGNLDPDLSAEIMRLFEQFSQVGVTMLIASHDIALIRRLGHRVLGLEQGHLVYD
                     GAL"
     gene            1357..>1623
                     /locus_tag="PKZ52_18910"
     CDS             1357..>1623
                     /locus_tag="PKZ52_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61898.1"
                     /translation="MSAFSLQKTANIEAWKAHHRSSCVESLQRILRAPLQSLLTALAL
                     AIALMLPAALLVALNNIAQLGERWDASPLQHRSIGRALGRQPPAV"
BASE COUNT          344 a          505 c          475 g          299 t
ORIGIN      
        1 ccctgcagga aaacctgcgc aatttactgc tgccggtgga aaagccgctg gctattcccg
       61 ccgataaaaa gccctttgtg attctggtgg tgggggttaa tggcgccggt aaaaccacca
      121 ccatcggcaa actcgcccac cggtttttaa gcgaaggcaa gagcgtgatg ctggcggcgg
      181 gggatacctt tcgggcggcg gcggtggagc aactgcaggt gtggggggag cgcaacaagg
      241 tgccggtgat tgcccagcac acgggggccg atagcgcttc ggtcattttt gatgccttaa
      301 acgccgcccg cgctcgccaa gtggatgtat taattgccga taccgccggg cgcctgcaca
      361 acaaaagcca tttaatggaa gagctgagca aagtgaagcg ggtgctggcc aaaatcgacc
      421 cggcaagccc ccacgaaacc ctgctggtat tggacgcagg caccggccag aacgccgtta
      481 accagtgcga tcaattccac cagagcatcg gtgttaccgg ccttgcgctg actaagctgg
      541 acggcaccgc caaaggcggc attattttcg ccctcagcca aaaacacaaa atccccgtgc
      601 gctacatagg cgtgggggag accttagacg atctacaacc cttcgccgcg cggccttttg
      661 tggacgcctt atttggtaaa cagtccgccc aatgattgag tttcgccagg tcagcaaacg
      721 ctacggcgac ggccacgagg ccctcttggg ggtgaacttt agtatcgccc ccaaggagat
      781 ggtgtttttg accgggcact caggcgcagg caaaagcacc ttgatgaaat taattctgct
      841 catggagcgg gccacttccg gccaggtact ggtggccgag cagaacctgg ccaaactgcg
      901 cagcgggcag atcccgtttt tgcgccgtca aatcggtgtg gtatttcaaa accaccaact
      961 gctgttcgac cgcagcgtct tccacaacgt ggccctgccc ctgcaggtgt gcggcataga
     1021 ccccaaagac agcgcccgcc gggtgcgcgc ggcgcttgat aaggtgggcc tgttggaacg
     1081 ggaaaaggcc aaccccatcg ccctctccgg cggcgagcag cagcgtgtgg ccatagcccg
     1141 cgccctggtg cacaaaccca aaatcctcct ggcggatgaa cccaccggca acctggaccc
     1201 agacctctct gctgaaatca tgcggctgtt tgagcagttc agccaggtgg gtgtgaccat
     1261 gctgatcgcg agccacgata tagccctgat ccgccgcctg ggccaccggg tgctgggcct
     1321 ggagcagggc catttggtgt acgacggagc cctctgatgt ccgccttcag cctgcaaaaa
     1381 accgccaaca tagaagcctg gaaggcccac caccgcagct cctgcgtgga aagcctgcag
     1441 cgtattctgc gcgcgcccct gcagtcatta cttaccgccc tggccctggc catagcactt
     1501 atgctgcctg ccgccttact agtggccctg aacaacatcg ctcaattggg cgagcgctgg
     1561 gacgccagcc ccctacaaca tcgctcaatt gggcgagcgc tgggacgcca gcccccggct
     1621 gtc
//
LOCUS       DAOQGS010000796         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_56203_length_1620_cov_5.178914, whole genome shotgun sequence.
ACCESSION   DAOQGS010000796 DAOQGS010000000
VERSION     DAOQGS010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56203_length_1620_cov_5.178914"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKZ52_18915"
     CDS             <1..436
                     /locus_tag="PKZ52_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNG61899.1"
                     /translation="IIDRASMARYTRSFGLMLRAGVPLNFALTLCARAIDNPYLAQKI
                     DAIRRGVERGDSLIRTHAAAQMFSPLVLQMIAVGEESGQVEQLLTDVAEFYEREVDYD
                     LKTLSDKIEPILIVIMAVFVTILALGIFLPMWDMYEVQKSAM"
     gene            complement(624..1472)
                     /gene="nadC"
                     /locus_tag="PKZ52_18920"
     CDS             complement(624..1472)
                     /gene="nadC"
                     /locus_tag="PKZ52_18920"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNG61900.1"
                     /translation="MNITLNDILPSIPPLVANSLAEDIGPGDISAELIPADRKAQAFV
                     ITREDCVVCGQPYFDEVFKQLGGVSIHWLVAEGAQVAANTRLVELTGPARVLLSGERC
                     ALNFLQTLSGTATTTAHYVTLVDNASFKLLDTRKTIPGLRLAQKYAVAVGGGYNHRIG
                     LYDAFLIKENHIAACGSITEAVNQARLLAPEKPIEVEVETLQQLQEAINAKANRVMLD
                     NFSLEDTLRAIEIARGQCEIEISGNLTADNIPALLDKPVDYVSSGALTKHLRAIDLSM
                     RLQLIY"
BASE COUNT          377 a          376 c          441 g          426 t
ORIGIN      
        1 tattattgat cgcgcctcta tggcgcgcta cacccgcagt tttggtttga tgctgcgcgc
       61 gggtgtgccg cttaattttg ctttaaccct ctgtgcaagg gcgattgata acccttattt
      121 agcgcagaaa attgatgcga ttcgccgcgg ggtagagcgc ggtgatagct taattcgcac
      181 gcacgccgcc gcacaaatgt tctcaccact ggtattgcaa atgattgcag tgggggagga
      241 gtctggccag gtggaacaac tgctcaccga cgtggcggaa ttttacgagc gggaagtgga
      301 ttacgactta aaaaccctca gcgataaaat tgagccgatt ttaattgtaa ttatggccgt
      361 atttgtcact attttagcgc tggggatttt tctgcccatg tgggatatgt acgaggtaca
      421 aaaaagcgcg atgtaaaaac aggcatttaa tgccgcagtt attaacccgg cggctaaata
      481 gatcgtcctg atctatttag ccttcgctgc cctaaaactg gcgaaaatgc gtggatttcc
      541 ttgtttgggc agctcacggc ggtttgcacc tttggagcgg catccatgcc gccattatta
      601 actcggcact aaatgccgcc gggttaataa atcagctgca agcgcatgga caaatcaatg
      661 gcccgcaggt gtttggtaag cgcgccggag gagacataat ccaccggttt atccagcagg
      721 gcagggatat tatcggcggt taaattgccg gatatttcta tctcacattg gcccctggct
      781 atttcaattg cccggagcgt atcttctaag gagaaattat ccagcatcac gcggttggcc
      841 ttagcattaa tggcctcctg tagttgctgc agagtttcca cttccacttc aatgggtttt
      901 tcaggcgcga gtaggcgggc ttggttgacg gcctcggtaa tgctgccgca ggcggcgata
      961 tgattttctt taattaaaaa ggcatcatac aaaccgatgc gatggttata gccgccgccc
     1021 accgccacgg catatttttg cgctaggcgc aggccgggga tggttttgcg ggtatctaat
     1081 aatttaaaac tggcgttatc caccagtgtc acataatggg cggtggtggt ggcggtgccg
     1141 ctaagggttt gtaaaaaatt taacgcgcag cgctcgccac ttagtaacac gcgggcgggg
     1201 ccggtcaatt ccaccaggcg cgtattggct gcaacctggg cgccctctgc caccaaccaa
     1261 tgaatactga cgccgcctaa ttgtttaaat acctcatcaa aatagggctg gccacacacc
     1321 acacagtcct cccgcgtgat aacaaatgct tgcgccttgc gatcggcagg aattaattcc
     1381 gcgctgatgt cgcctgggcc tatgtcttcg gcaaggctat tggcaactaa aggtgggatg
     1441 ctggggagta tgtcgttgag ggtgatattc ataagtgtgg ggtgattgaa ttaatggagg
     1501 gtttgtatgt gcaatacgag tgaattgttt gcgcctggct gctgattaat tgcaccgggc
     1561 tttaacagct tgttgaaata ctaccttgct agcactggcg gcgttaaatt tggcgaaaaa
//
LOCUS       DAOQGS010000797         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_57064_length_1607_cov_4.868557, whole genome shotgun sequence.
ACCESSION   DAOQGS010000797 DAOQGS010000000
VERSION     DAOQGS010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57064_length_1607_cov_4.868557"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /gene="gpmI"
                     /locus_tag="PKZ52_18925"
     CDS             complement(<1..1381)
                     /gene="gpmI"
                     /locus_tag="PKZ52_18925"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNG61901.1"
                     /translation="MSAKQPVVLIILDGFGYNESAQYNAIANAQAPLWQSLWNKGPRT
                     LIGTSGMAVGLPEGQMGNSEVGHMTLGAGRVVYQNFTRINKAISDGDFFTNPVYCAAV
                     DKAVSSGKAVHVMGLLSDGGVHSHQDHILAMLKLAAERGAQKLYLHAFLDGRDTPPRS
                     AEAALKEADATFAALGVGRTASLIGRFYALDRDNRWERVSQAYNLITASQAEFTAADA
                     VSGLQAAYERGENDEFVKATRIGEPVQVEDGDSVIFMNFRPDRAREITNCFVDDQFSG
                     FTRSQRPKLADFVMTTEYSADIHTSCAYPPDDVKNSFGEVLAANHKTQLRIAETEKYA
                     HVTFFFSGGREEVYEGEERILVPSPKVTTYDLQPEMSAPEVTEKLVAAIKSQRFDAII
                     CNYANCDQVGHTGVYEAAVKAVEAVDACLVEVLAAVKEVNGHTLITADHGNVELMFDE
                     TSGQLHTQHT"
BASE COUNT          333 a          419 c          458 g          397 t
ORIGIN      
        1 tggtgtgctg ggtgtgtaat tggccggagg tttcatcgaa cattaattcc acattgccgt
       61 ggtcggcggt aattaatgtg tggccgttca cttcttttac cgctgctaat acttccacca
      121 aacaggcatc caccgcttcc actgctttta ccgccgcttc atatacgccg gtgtggccca
      181 cttggtcgca attggcgtag ttgcaaataa tggcgtcaaa gcgctggctt ttaatggcgg
      241 cgactaattt ttccgtcact tctggcgcgc tcatctccgg ctgtaaatca taggtggtga
      301 ccttgggtga gggtactaaa atgcgttctt cgccctcgta aacctcttcg cggccgccgc
      361 taaaaaagaa ggtaacgtgg gcatattttt cggtttcggc aatgcgcaat tgggttttgt
      421 ggttggcggc gagcacttcg ccaaaggagt tttttacatc atccggcgga taggcgcagc
      481 tggtgtgaat atcggcggaa tattcggtgg tcataacaaa atcggcgagt ttggggcgct
      541 ggctgcgggt aaaaccgctg aactgatcat ccacaaaaca gttggtaatt tcccgcgcgc
      601 ggtcggggcg gaaattcata aaaatcacac tgtcgccatc ttccacctgt actggctcac
      661 cgatgcgggt ggctttgaca aactcgtcgt tttcgccgcg ctcataggcg gcctgcaggc
      721 cgctcacagc gtcggccgca gtaaattcag cttggcttgc ggtaattaaa ttgtacgcct
      781 ggcttacacg ctcccagcga ttgtcgcggt caagggcata aaagcgcccg atgaggcttg
      841 cagtgcggcc cacacccaga gctgcgaagg tggcgtccgc ctctttaagg gcggcttcgg
      901 cactgcgggg cggtgtatcg cggccatcta agaaggcatg gaggtagagt ttttgggcgc
      961 cgcgctcggc ggcgagtttt agcatggcca gaatatgatc ctggtggctg tgcacaccgc
     1021 cgtcggagag caggcccatc acgtgcacgg ccttgccgct gctgacggct ttatccaccg
     1081 ccgcgcaata aactggattg gtaaaaaaat cgccgtcgct gatggcctta tttatacggg
     1141 tgaaattttg gtacaccact cggccagcac ccaaggtcat gtggcccact tcagagttcc
     1201 ccatctgccc ttcgggtaaa cccaccgcca taccggaagt gccaattaag gtgcgcggcc
     1261 ctttgttcca gaggctttgc cagagggggg cttgggcatt ggcaatggcg ttgtattgcg
     1321 ctgattcgtt gtagccgaag ccgtcgagga tgatcaaaac tactggttgt ttagcggaca
     1381 tgctgaagcc ttttggaatg cgaggaatta cacaaaggta ccggtggcga agggtataga
     1441 tgaaattgcc gtaaagcgcc cttataaaat cgctacctaa acgccgccgc gcattgagcc
     1501 tggagtctgg ccttaagcgc ggtgtttaga tcggacgagc cattttatac acatgctgca
     1561 gccttcgccc agtgttcatg tatactgcgc gcctttttac agcggcc
//
LOCUS       DAOQGS010000798         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_57276_length_1603_cov_4.383721, whole genome shotgun sequence.
ACCESSION   DAOQGS010000798 DAOQGS010000000
VERSION     DAOQGS010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57276_length_1603_cov_4.383721"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PKZ52_18930"
     CDS             complement(<1..1127)
                     /locus_tag="PKZ52_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNG61902.1"
                     /translation="MIAKLSKSILTALGLFSSAALYAEDRFVWVEAESGAEYNPIVVK
                     SDPGASQKIYLGSWKWADYTTRNDGAGKITYSVYIPEAGTYKIWARMRAPANIRPFDI
                     SKDNGNLSDNSAWTRWAATPSLTTWGWSDSGYTGTFTAGTHTIYLAQREGGPNLHLDK
                     ILITNNTSFVPTGAGEPETIPNIANPYKSNTVEKYGQLRVSGGKLLDKNNQPVQLQGV
                     SAHGLQWFPLVGSQTIAYSAEFFGAEAVRLAMYVEDFAPTDSSDYWGGYTADKASMMQ
                     RVDQAINDAVNAGIYVLVDWHIHNTPGKYTAESTQFFTDIAKRYGHLPNIIYEICNEP
                     VSVSWSSGIKPYANTVISAIRAIDPDGVVIVGTPNWSQDVDA"
BASE COUNT          411 a          322 c          411 g          459 t
ORIGIN      
        1 gcatcaacgt cttggctcca gttgggtgtg cccacaatga cgacaccgtc agggtctatg
       61 gcgcggatag cactgataac agtattggcg taaggcttga tgcctgatga ccagctaacg
      121 ctgaccggct cgttacaaat ttcataaata atattcggta aatggccgta gcgtttggca
      181 atatcggtaa aaaattgtgt ggattctgcg gtgtatttgc ctggagtgtt atggatgtgc
      241 caatccacca atacataaat accggcattt acagcatcgt taatggcttg atccactcgt
      301 tgcatcatgg atgctttatc agcggtgtat ccaccccaat agtctgaaga gtcagtgggg
      361 gcaaaatctt caacatacat agccaagcgc acggcttctg cgccgaagaa ttcagcgcta
      421 taggcaatgg tttggctgcc tactagcggg aaccactgta aaccgtgtgc acttacgcct
      481 tgtaattgca cgggttggtt atttttgtcc aataatttgc cgccactaac gcgcagctgg
      541 ccgtattttt ccacggtgtt ggatttatag gggttggcaa tattaggaat ggtttccggc
      601 tcgcctgcac cagtgggtac aaagctggtg ttattggtga tgagaatttt atccaggtgg
      661 agatttgggc cgccttcacg ttgggctaaa taaatagtgt gggtgccggc ggtaaatgtt
      721 ccggtataac ccgaatcgct ccagccccaa gtggttaaag agggggttgc tgcccagcgt
      781 gtccaggcgc tgttatcgct taaattgccg ttgtctttgc tgatatcaaa agggcgaata
      841 ttggcgggcg cgcgcattct ggcccaaatt ttataagtgc ctgcttcagg aatataaacc
      901 gaataagtaa ttttgccggc gccgtcgttg cgggtggtgt aatcggccca tttccaggag
      961 cctaaataaa ttttttgtga ggcacccggg tcagatttaa ccactatggg gttgtattca
     1021 gcaccggatt ccgcctcaac ccaaacgaag cgatcttcag catacaaagc ggctgagcta
     1081 aataggccta gtgccgtcaa tatacttttc gagagttttg caatcatgga aaacctactt
     1141 gttgaatggt gaatttggtc ttgagtgatt ggataatact cgtacatttt ttaatctgca
     1201 aaaaaatgtt tagtaaaaaa gtatgggatt ttgctggggg aacagagcgg tttttgtcgg
     1261 ttttttggcg tatttgtttt ttaagagggg agagatttag aggaggtcaa ataattgccg
     1321 caggcttcta accctgggcg ataaaaatta tccgcttata ctcgaattcg agttgttata
     1381 tacaaaacga ttttcgacgc caaaaatgcc ttggcggttg tataagagca aaagctgatt
     1441 taataactgg attttattag aaatcgaact gctgcataaa acagtctaaa cagaagccaa
     1501 cttttaaagg ctttgtgaag gctgtaactc ttttgtggtc aaaatggctg gctgccccag
     1561 cgtcgggaaa ttgcgtagcg actatttgca cggtgttata agg
//
LOCUS       DAOQGS010000799         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_57862_length_1593_cov_5.987646, whole genome shotgun sequence.
ACCESSION   DAOQGS010000799 DAOQGS010000000
VERSION     DAOQGS010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57862_length_1593_cov_5.987646"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1522
                     /locus_tag="PKZ52_18935"
     CDS             182..1522
                     /locus_tag="PKZ52_18935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNG61903.1"
                     /translation="MPLARLRSFNMHLKPEWILGIAAVVSLLLIVIPKDLLTRTDQLN
                     LQEHRAVLFADAYSGGNSSATWIDEGQKHWQCKLGQNLPAPYCSIRLALTDEKGGGLD
                     LSRALSLTLWIDYKGNGSHLRIYLRNRGIKHYVPGDDLSEKYNMVEIPVQDMAQGFTI
                     NMDEFGVADWWLTQRNIPLKEARPEFNNISDMEIQTGSLMVSGDHEVTLRAMSWETPF
                     ISELQLYRLVTILWTGLIITFLLFRITAMHMALNRQQAKQQELIAINGILSTQNRRFE
                     DLAKTDQLTGLHNRVAMQEALYQGLNNWKHHREAFSMVLLDIDHFKKINDNLGHDAGD
                     EVLKLVAGVLKSSVRKSDFLARWGGEEFVLLYPNTSQMEALAAAESLRQKIASALNPW
                     GIVITASFGVATLVSGDLDLLFKSADEALYRAKNSGRNCVMGDLRIGINAQNLS"
BASE COUNT          408 a          438 c          373 g          374 t
ORIGIN      
        1 gtgcaactcg tctgcgccag ggtgcgcagg cgggtttgct tttaccagat ccctcatttg
       61 catccctttc gattgctcac accctaacac gctgcagtgc ggcatccctg cgttggcgct
      121 gactcacttt gccaactaga ctcaaaaagt acgattaaac ctgagcttct agggaaccat
      181 catgccgctt gcacgcttgc gctcttttaa tatgcacctt aaaccggaat ggattttggg
      241 catcgcagcg gtagtctccc tcctacttat tgtcatccca aaggatttgc tgacccgcac
      301 cgaccaatta aatctgcagg aacaccgggc cgttttattt gccgatgcct actccggcgg
      361 taacagctca gccacctgga tcgatgaagg gcaaaaacac tggcaatgca aactgggcca
      421 gaatttaccc gccccctatt gcagcatacg cctagccctc accgacgaaa aaggcggcgg
      481 cttagacttg agccgcgccc tgtcactcac cctttggatt gattacaaag gcaatggcag
      541 ccacctgcgt atttacctgc gcaatcgcgg cataaaacat tatgtgcccg gcgacgacct
      601 cagcgaaaaa tacaatatgg tggaaatccc cgtgcaggac atggcccagg gtttcaccat
      661 taatatggat gaattcggcg tggccgattg gtggctcacc cagcgcaata ttccgctcaa
      721 agaagcgcgg cccgaattta ataatatttc cgacatggaa attcaaaccg gctccttaat
      781 ggtttctggc gatcacgaag tcaccctaag agccatgagt tgggaaacgc cgtttattag
      841 cgaattacaa ttgtatcgcc tggtcacgat tctctggacg ggtttaatta ttaccttcct
      901 gttatttcgc atcaccgcca tgcatatggc cctgaatcgg caacaggcaa aacagcaaga
      961 actaattgcg atcaatggaa ttttaagcac ccaaaaccgc cgctttgagg accttgccaa
     1021 aaccgatcag ctcaccggcc tgcacaatcg cgtcgccatg caagaggcgc tctatcaagg
     1081 cttaaataac tggaagcacc accgcgaagc tttttccatg gtgctactgg atattgacca
     1141 ctttaaaaaa attaacgata acctcggcca tgatgctggc gatgaggtac tcaaattagt
     1201 ggctggtgtt ttaaaaagca gtgtgcgcaa atccgatttt ctggcgcgct ggggcggcga
     1261 agaatttgtt ttactctatc ccaataccag ccaaatggaa gcgcttgcgg cggcagaatc
     1321 tttgcggcaa aaaatagcct ccgccctcaa cccatgggga attgtgatca ccgccagctt
     1381 cggcgtggcc accctcgtct caggtgattt agacctatta tttaaatccg ccgacgaagc
     1441 cttgtaccgc gccaaaaaca gcgggcgcaa ttgtgtcatg ggcgatttgc gcatcggtat
     1501 caacgctcaa aacctctcct aaccggcagt gcataaactt agggaacctc tgaaaaatgt
     1561 aggcggcgaa gctctggcta ggcgaatttg gca
//
LOCUS       DAOQGS010000800         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_58470_length_1584_cov_4.353826, whole genome shotgun sequence.
ACCESSION   DAOQGS010000800 DAOQGS010000000
VERSION     DAOQGS010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58470_length_1584_cov_4.353826"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="PKZ52_18940"
     CDS             complement(<1..>1584)
                     /locus_tag="PKZ52_18940"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HNG61904.1"
                     /translation="SPHFGERQARRWLDLARYADSAGFQNDQTRPNTWRYRDYVINAF
                     NDNKPFDQFIREQLAGDELFPDNQDAKIATGFLALYPDNYNSRDLVQRKYQIETDITD
                     LVGETFLASTIGCARCHNHKIDKISQKEYFQLQAFFANLIVNEKTPVLKGTETQAEKD
                     YAQAQAAYQEATKDIRAKQKALLDPIREKARKYHNERYLTDSRESIFKPEKDWTPLDR
                     WVNYRTNAVASERDIVAYLKQSATEKDGSEFNAANIPRWEEYEKLTAELKKFDSLKPI
                     KGSSTYTSVTELGPIAPPTRIRFTGIHEKPLDEVQPGLPALWAGDTKLDIVPTAKTTG
                     RRTALANWLASNDNPLTPRVYVNRVWAQYFAKGIAANPGDFGRAGPKPTHPELLDYLA
                     GGFVKDGWNIKNLHRQILLSSTYRQSSEERADVQKVDPDNSLLAVYPRKRLEAEEIRD
                     ALLSASGKLDDTVGGPSVFPQLPPTVVVANTLNNNGGGLLWETSKDPKDFNRRSIYTF
                     VRRSLPYPLTASFDPADPS"
BASE COUNT          384 a          360 c          406 g          434 t
ORIGIN      
        1 gggatggatc ggccgggtca aatgatgcag ttaatggata aggcaaactg cggcgcacaa
       61 aggtgtaaat gctgcggcga ttaaagtctt tcgggtcttt tgaagtttcc cacaacaaac
      121 cgccgccatt attattcagt gtattggcaa ccactacggt gggtggtaat tgcgggaaca
      181 ctgaagggcc accaacggta tcatccaatt tgccagacgc cgacaggagc gcatcgcgaa
      241 tttcttctgc ctctaaacgc ttgcgtgggt atacagcgag cagactatta tccgggtcta
      301 ctttctgcac atcggcgcgc tcctcagaag attgacgata ggtactggag agcaaaattt
      361 ggcgatgtag gtttttaata ttccaaccat cttttacaaa accgccggct aaataatcca
      421 ataattcagg gtgtgtaggt ttagggcctg cgcgaccaaa atcacctggg tttgcggcta
      481 tacccttagc aaaatactgc gcccacacac ggttgacata aacgcgcggc gttaatgggt
      541 tatcgttact ggcaagccag ttagccaaag cagtacgacg gcctgtggtt tttgcagtcg
      601 ggacaatatc caacttagta tcgccagccc acaaagcagg taaacctggt tggacctcat
      661 ctaatggctt ctcgtgaatc ccggtgaagc gaatacgagt cggtggcgct attgggccta
      721 attcagtaac cgacgtgtag gtgctggagc ccttgatcgg ttttaatgaa tcaaatttct
      781 tcagctcagc cgttagcttt tcgtactctt cccaacgcgg aatattagct gcattaaatt
      841 ctgagccgtc tttctctgtg gcagattgtt ttaagtacgc gacgatatct cgctcagatg
      901 ctactgcatt ggtgcggtag ttaacccaac gatcaagtgg cgtccagtct ttttctggtt
      961 taaagatcga ctcgcgactg tcggttaagt aacgctcatt gtggtattta cgggcttttt
     1021 ctcggatggg atcaagcagt gccttttgtt tagcgcgaat atctttggtc gcttcttgat
     1081 acgcagcctg ggcctgagcg taatctttct ccgcctgagt ttcagtgcct tttaagacag
     1141 gtgttttttc attcacaatc aaattggcaa aaaaggcctg cagttggaaa tattctttct
     1201 ggctaatttt gtcaattttg tggttgtggc agcgggcaca gccgatggtt gaagccagga
     1261 aagtttcacc cactaaatcg gtgatatcag tttcaatctg gtatttacgc tgtaccaagt
     1321 cgcgggagtt gtagttatca gggtaaagcg ccaaaaagcc ggtggcgatt ttggcatcct
     1381 ggttatcagg aaacagctcg tcgcccgcca attgctcgcg aataaattgg tcaaacggct
     1441 tattatcgtt aaaggcgtta atcacatagt cgcgatagcg ccaggtgtta ggacgggttt
     1501 ggtcattttg gaaaccggcc gaatcggcat aacgggccaa atctaaccac cggcgggctt
     1561 ggcgctcacc aaagtgtgga gatg
//
LOCUS       DAOQGS010000801         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_59361_length_1570_cov_3.656766, whole genome shotgun sequence.
ACCESSION   DAOQGS010000801 DAOQGS010000000
VERSION     DAOQGS010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59361_length_1570_cov_3.656766"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKZ52_18945"
     CDS             <1..316
                     /locus_tag="PKZ52_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNG61905.1"
                     /translation="QSPEDREKLDGLYECILCACCSTSCPSFWWNPDKFIGPAGLLQA
                     YRFLADSRDTATAQRLGKLDDPFSVFRCHGIQNCVAVCPKGLNPTRAIGHIRSMLLSR
                     AT"
     gene            451..>1570
                     /locus_tag="PKZ52_18950"
     CDS             451..>1570
                     /locus_tag="PKZ52_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNG61906.1"
                     /translation="MQNNLMELLWGTSHISGGNAAYVEEVYEAFLRDPSSVSSDWRDY
                     FEKLPQNGHGGSPDVPHSEVIEYFELLGKNRARPLVAPGSGAIDVKHERKQVELMQLI
                     NAYRLSGHKKAKLDPLGLQKPATDIEELDLHFHGLTNNDLDLVFQTGDLSFGKAEASL
                     REIVSDLEKTYCGTIGAEVMHITRSKERRWLLSRLESSRAKAVLSPEAKRALLERLTA
                     AEGLERHLDSKYPGTKRFGLEGGESLIPMLDALIKRAGSFGAKEMVLGMAHRGRLNTL
                     INIFGKSPSDLFGEFEGKKLVTTSGDVKYHQGFSSNVMTPGGEMHIAMAFNPSHLEIV
                     SPVVEGSVRARQDRRQDPKGHTVVPVVIHGDAAFAGQGV"
BASE COUNT          337 a          407 c          452 g          374 t
ORIGIN      
        1 gcagtcgcct gaggatcgcg agaagctgga cggcctgtac gagtgcattc tgtgcgcctg
       61 ttgttcaacc agttgcccat cattttggtg gaacccggat aaatttatcg gccctgcagg
      121 cctgttgcag gcctatcgat ttttggcgga cagccgggat acagctacgg cgcagcgctt
      181 gggtaagttg gatgatccgt tcagtgtatt tcgctgccac ggcatccaga actgtgtggc
      241 ggtgtgcccc aaaggcttaa accccacccg cgccataggt catattcgct ccatgttatt
      301 gtcccgagcg acctaggctg ttctagtctt tagtttgttt ttagccccgc ttttgcgggg
      361 ctttatttga gtaggccaca gatgtggttt gatccattaa tggcaacagg ctcagtacta
      421 cggcgagcta tataaaggta ggttggcatc atgcaaaaca atttgatgga gcttctttgg
      481 ggaacgtccc acattagcgg tggcaatgcc gcttacgttg aggaggttta tgaggcgttc
      541 cttcgcgatc caagttctgt atcgtcagat tggcgggatt attttgaaaa gctaccgcaa
      601 aacggccacg gcggcagccc cgatgtgccc cactcagagg tgatcgagta ttttgagttg
      661 ctgggtaaaa accgcgcccg accactggtt gcgcccggct ctggtgccat tgatgtcaag
      721 catgagcgca agcaagttga gttgatgcag ttgatcaacg cctaccgttt gagtggtcac
      781 aaaaaagcca agctcgaccc attgggcttg caaaagcccg ccacggatat tgaagagctg
      841 gatctgcact ttcacggcct tactaacaac gacctagacc tggtgtttca aaccggcgat
      901 ctctcctttg gcaaagccga agcgagcttg cgggagattg tcagcgatct agaaaaaacc
      961 tattgcggca ccatcggtgc cgaagttatg catatcacgc gttctaaaga gcgccgttgg
     1021 ctgctcagcc gcctagaatc cagccgtgcc aaggcggttt tgtcgccgga agccaagcgc
     1081 gcactgcttg agcgactcac cgccgcggag ggccttgagc ggcatttgga cagtaaatac
     1141 cccggcacca agcgcttcgg cctggagggt ggggagagtt tgatccccat gctcgatgcg
     1201 ctgatcaagc gagccggttc cttcggcgct aaagaaatgg tgttgggtat ggctcaccgg
     1261 ggccgcctga acacgctgat caatatcttt gggaaaagcc cgtccgacct gttcggcgag
     1321 ttcgaaggta aaaaattggt gaccacgtcc ggcgacgtga agtatcacca gggttttagc
     1381 tccaacgtga tgacccccgg cggtgaaatg cacattgcca tggcctttaa cccgtcccat
     1441 ttggagattg tatcgcccgt ggtggaaggc tctgtgcgcg ctcgccaaga tcgccgtcag
     1501 gatcctaaag gccacacggt ggtacctgtg gtaattcacg gtgatgctgc ctttgcaggt
     1561 caaggtgtgg
//
LOCUS       DAOQGS010000802         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_59754_length_1564_cov_3.766070, whole genome shotgun sequence.
ACCESSION   DAOQGS010000802 DAOQGS010000000
VERSION     DAOQGS010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59754_length_1564_cov_3.766070"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKZ52_18955"
     CDS             <1..430
                     /locus_tag="PKZ52_18955"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNG61907.1"
                     /translation="DPVCIKEACRVVIAMVQQIACGAENWGVMHLRSAEPCFEAEFAD
                     HLVRQLKKDNWPAARLDVSKGPSVLYGPMSVLAGRRLTDAFGVQMRSWRLGVKALLQR
                     WMDERRQLELALSSNPPLSSDAQAGLNNSPQSAPTQHSAL"
     gene            complement(598..>1564)
                     /locus_tag="PKZ52_18960"
     CDS             complement(598..>1564)
                     /locus_tag="PKZ52_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNG61908.1"
                     /translation="EHSGLSPEQVSHERTGIIMGSGGASTANVVESADIIRSKGVKRV
                     GPYRVTQTMGSTASACLATPFKIKGVNYSISSACATSAHCIGNAMELIQLGKQDVVFA
                     GGGEEEHWTLTGLFDAMGALSTKYNDTPGQASRAYDADRDGFVIAGGGGCLVVESLDH
                     ALARGATIYAELTGYGATSDGYDMVAPSGEGAARCMRQAIAMTDASPIDYINSHGTST
                     PVGDLAELRAVKEVFGAGQVPAISSTKSLTGHSLGATGVQEAIYSLLMMQHNFVAASA
                     NIGTLDPEAENMSIVLQSREAVLNKVMSNSFGFGGTNATLIFQRY"
BASE COUNT          320 a          432 c          493 g          319 t
ORIGIN      
        1 cgacccggtt tgtataaaag aggcgtgccg ggtggtgatc gccatggtgc agcaaatcgc
       61 ctgtggcgcc gaaaactggg gtgttatgca cctgcgttcg gcggaaccct gctttgaagc
      121 ggaatttgcc gaccatttgg tgcggcagct gaaaaaagat aactggcccg ccgcgcgctt
      181 ggatgtaagt aaggggccga gcgtcttata cggccctatg agcgtgctgg cgggcaggcg
      241 cctgacggat gcctttggtg tacagatgcg ctcttggcga ctgggggtta aggcgttgtt
      301 gcagcgttgg atggatgagc gcaggcaatt ggagctggcc ctcagttcta atccgccgct
      361 cagctcggac gcacaggcag gccttaataa ttcaccccag agcgcgccca cacaacacag
      421 cgcgctctaa tgcacggcaa ctaaaatgca cggcacctca aattccgcag caccttaaac
      481 tcttggcaac taaaaaagcc ccagttgaac ccctaggcgc tctcgctagg ggttcaactg
      541 gggcttttag atggatacag cctgtgcact cgcccaggcc gatccacaac gcatcgatta
      601 ataacgctga aaaatgagcg tggcgttggt gccgccaaag ccaaagctat tggacatcac
      661 cttgtttaac accgcctcgc ggctttgcag cacgatggac atgttctcgg cttctgggtc
      721 tagggtgccg atattggccg atgcggcaac aaagttgtgc tgcatcatca gcaaactgta
      781 gatagcttcc tgtacgccgg tggcacccaa tgagtggcca gtcagggatt tggttgaact
      841 gatggcgggc acttggcctg caccgaacac ttctttcacc gcacgcaatt ccgccaaatc
      901 gcccactggg gtgctggtgc cgtgggagtt aatgtagtcg atggggctcg cgtcggtcat
      961 ggcgatggcc tggcgcatac agcgagcggc gccttcccct gaaggggcca ccatgtcata
     1021 accatcggag gtggcgccgt agccggtgag ttccgcatag atagttgcgc cgcgggccag
     1081 ggcgtggtcg agggattcca ccaccaaaca gccgccgccg ccagcgatca caaacccgtc
     1141 gcggtcggcg tcataggcgc gggaggcttg gccgggggta tcgttgtatt tggtggagag
     1201 ggcgcccatg gcatcgaaca ggccggtgag ggtccagtgt tcttcttcgc cgccacctgc
     1261 aaataccacg tcctgtttac ccagctggat cagctccatg gcgttgccaa tacagtgggc
     1321 gctggtggcg caggcggagg aaatggagta gttgacgcct ttaattttaa acggcgtagc
     1381 caggcaggcc gaggcggtgg agcccatggt ttgggtaacc cggtaggggc cgacgcgttt
     1441 tacacccttg gagcgaataa tatcggccga ttccaccaca ttggcggtgg aggcgccgcc
     1501 ggaacccata ataatgccgg tgcgctcgtg ggacacctgc tcagggctga ggccggagtg
     1561 ttcg
//
LOCUS       DAOQGS010000803         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_59845_length_1563_cov_3.336870, whole genome shotgun sequence.
ACCESSION   DAOQGS010000803 DAOQGS010000000
VERSION     DAOQGS010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59845_length_1563_cov_3.336870"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /gene="pseI"
                     /locus_tag="PKZ52_18965"
     CDS             complement(<1..1029)
                     /gene="pseI"
                     /locus_tag="PKZ52_18965"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HNG61909.1"
                     /translation="MNRPIYLGSRGVGAGYKPFIIAEMSGNHNQSIERALAIVDAAAK
                     AGVDALKIQTYTPDTMTIASNRPEFLVQDPGKLWAGKSLYQLYQEAYTPWEWHAEIFA
                     RCRHWGIEGFSTPFDESAVDYLEHLQVPFYKVASFENTDLPLLAAIAKTGKPVIMSTG
                     MARINELNDAVSCLRDNGCRDLILLKCTSTYPTSPENTHINTIPHMAQLFNCPVGLSD
                     HTMGVGVAVASIALGACVVEKHFTLARADGGVDAAFSLEPAEMQQLVVEAERAYLALG
                     QVNYGQGENEDKMRLYRRSIYVTQPIKKGELFSRDNTQIIRPSLGLAPKYYQQILGKA
                     ARCDIAAEE"
     gene            complement(1026..>1563)
                     /locus_tag="PKZ52_18970"
     CDS             complement(1026..>1563)
                     /locus_tag="PKZ52_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61910.1"
                     /translation="GGTDPKELSHIWFHLLAPVANEFADITFLLASNAVGLNSLAKKI
                     AIHQQTTGGIELLVDVRHMPQLLLEHDLAIGGAGVSALERAVLGLPSLLLTLADNQII
                     QAQALITIGCARNLGDWQQVPSISAQQAALAVVLAAKNNYVDYKTMSQQGFNMIASEH
                     PYQAINEFLTHQRAEANA"
BASE COUNT          419 a          408 c          337 g          399 t
ORIGIN      
        1 ctcttcggct gctatatcgc agcgggccgc tttacccaaa atctgttggt agtatttcgg
       61 tgccaagccc aaagagggtc gaataatttg ggtattatcg cggctaaata actcaccttt
      121 ttttatcggt tgcgtgacat aaatagagcg acgatacaaa cgcattttat cctcgttttc
      181 accctggcca taattaacct gcccgagtgc taaataagcc cgctcagctt ccaccactaa
      241 ttgctgcatt tctgcaggct ctaaactaaa tgccgcatcc acaccgccat cagcccgcgc
      301 cagggtaaaa tgtttttcca ccacgcaagc accgagggca atactcgcta cagcaacgcc
      361 cacacccatg gtgtgatcgg ataaacccac cgggcaatta aataactgcg ccatatgggg
      421 gatggtatta atatgggtat tctccggtga ggttgggtag gtgctggtgc attttaatag
      481 gattaaatcc cggcagccgt tatcccgcag gcagctgacg gcatcattta attcatttat
      541 acgcgccata ccggtagaca taatcaccgg cttgccggtt ttagcaatgg ccgccaaaag
      601 gggtaaatcc gtattttcaa aagaggccac tttataaaaa ggcacctgta aatgctccag
      661 ataatccacc gccgattcat caaagggtgt actaaaacct tcaatacccc agtgcctgca
      721 acgggcaaaa atttccgcgt gccactccca aggggtatag gcctcttggt ataactgata
      781 gagggattta cccgcccaga gtttacctgg atcctgcact aaaaattccg gccggttgga
      841 ggcaattgtc atggtatccg gcgtataggt ttgaattttt aatgcatcca caccggcttt
      901 ggcagcggcg tctactatgg ctagggcccg ctcaatagat tgattatggt tgccggacat
      961 ttcagcaata ataaaaggtt tataccctgc gcccacacct cggctgccta aataaatcgg
     1021 cctattcatg cattcgcttc cgctcgctga tgagttaaaa attcgttgat ggcttgataa
     1081 gggtgctcac tggcaatcat attaaagccc tgttggctca tagttttata atcaacataa
     1141 ttattttttg ccgccaacac cacggctagg gccgcttgct gggcactaat actcggcacc
     1201 tgttgccaat cgcccagatt acgcgcgcaa ccaatagtaa ttaacgcctg ggcttgtatt
     1261 atttggttat ccgcaagagt gagcaataaa ctcggcaagc ctaaaactgc tcgctccaaa
     1321 gccgaaaccc cagcgccgcc gatggccaaa tcatgctcca ataacaactg cggcatatgg
     1381 cgcacatcaa caagcagttc aataccgcca gtggtttgtt ggtgaatggc tattttcttc
     1441 gccaaactat ttaaaccaac tgcattagaa gccaataaaa atgtaatgtc tgcaaattca
     1501 ttcgccaccg gcgctaataa atgaaaccaa atatgcgaca actctttagg gtccgttccc
     1561 cct
//
LOCUS       DAOQGS010000804         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_59964_length_1561_cov_4.341301, whole genome shotgun sequence.
ACCESSION   DAOQGS010000804 DAOQGS010000000
VERSION     DAOQGS010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59964_length_1561_cov_4.341301"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKZ52_18975"
     CDS             <1..188
                     /locus_tag="PKZ52_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNG61911.1"
                     /translation="ALRVHYKPFVRWIWLGALLMALGGTLAIIDKRYRLKVATTQGNT
                     IEPKAAIAIAAQARESV"
     gene            185..709
                     /locus_tag="PKZ52_18980"
     CDS             185..709
                     /locus_tag="PKZ52_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNG61912.1"
                     /translation="MKRLRLFIPLIVFVFLSALLYWGMSRNPDELPSVLIGKPLPEFA
                     LSSLNNPAERITKAQVVTGKPFILNVWASWCPTCKDEHPFLNKLSAMGVKIVGINYKD
                     DPDAAKAILARLNDPYSVNLLDPDGKLGIDLGVYGAPESFIVDANGMIVDKVVGAIDE
                     QIWQQQVAKYFNLN"
     gene            805..1227
                     /locus_tag="PKZ52_18985"
     CDS             805..1227
                     /locus_tag="PKZ52_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61913.1"
                     /translation="MQIGPGLNTLTQIQGAWRNSCHRVGIKESEGYRQDFLNINFTHF
                     EFTAKVYKDTSCTHLVTQWPARYRFIVGDQVLLPNHEKAFLLSLNEEADPADAWALSP
                     LNILQYKAGQLSLGRESLLGPSTTQLTSLDLDLSFSRR"
     gene            1233..>1561
                     /locus_tag="PKZ52_18990"
     CDS             1233..>1561
                     /locus_tag="PKZ52_18990"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNG61914.1"
                     /translation="MSKSLLYPEWANTRAITLAWPFPASDWSANLAQAQQCYAHMVAA
                     FSRVVPVWLLVHPSVELDSVKSALEKLNAHVARVELIHTINYNDTWMRDYGPLSCNTG
                     YIQFEFNG"
BASE COUNT          399 a          380 c          361 g          421 t
ORIGIN      
        1 gggccctgcg cgtccattac aagccgttcg tgcgctggat ttggctgggg gccctactta
       61 tggcgctcgg tggcaccctt gcaattatcg ataagcgcta ccgcttaaaa gttgccacta
      121 cccaaggcaa cacaattgaa cctaaggctg ctatcgccat tgcagctcaa gctcgggaat
      181 cagtatgaag cgtttacggc tatttattcc tctaattgtt tttgtatttc tctccgccct
      241 actttattgg ggcatgtccc gcaaccccga tgaattacct tccgtgctga tcggcaagcc
      301 actgccagaa ttcgccctgt cgagtttgaa taacccggcg gagcgaatta ccaaagcgca
      361 ggttgttacc ggcaagcctt ttatattaaa cgtctgggcg agttggtgcc ctacctgtaa
      421 agacgagcac ccctttttaa ataaattatc agccatggga gttaaaatag tcggcatcaa
      481 ttacaaagat gacccggatg cagcgaaggc tattttggct cggttaaacg atccctacag
      541 cgttaacttg ctggacccgg atgggaaatt agggattgat ttgggtgttt atggcgcccc
      601 tgagtcattt atcgtggatg caaatggaat gattgttgac aaggtggtcg gcgctattga
      661 cgagcaaatt tggcaacaac aagttgccaa atattttaac ctgaattagc aatgccatta
      721 attaattaca gcagactccg attagccgct atcgctagtg cacttacgtt aatactctgc
      781 ccacatgcaa acgcagataa cacggtgcaa attggccccg gccttaacac cttgacgcaa
      841 attcaaggtg cctggcgcaa tagctgtcac cgggtgggga taaaagaatc tgagggctac
      901 cggcaagatt ttttaaatat taatttcaca cactttgaat ttaccgccaa agtgtacaag
      961 gataccagct gcacacattt agtcacccag tggcccgcaa gataccgttt tatagtgggc
     1021 gatcaagtgt tactgcccaa tcatgaaaaa gcctttttac tcagcctaaa cgaagaggcc
     1081 gacccggcag atgcctgggc tctgtcgccc ctgaatattc tgcagtataa ggctgggcaa
     1141 ttaagcctag ggcgcgaaag tctattaggc cccagcacta ctcaactaac ctcccttgat
     1201 ttagatttat cttttagccg tcgttaatca ctatgtcaaa atcgttactg tatcctgagt
     1261 gggccaacac tcgtgccata acactcgcct ggccatttcc tgctagtgat tggagtgcca
     1321 atcttgccca agcccaacag tgttacgccc atatggtggc ggcgttttcg cgcgttgtgc
     1381 cggtttggtt attggtgcac cccagtgttg aattggattc ggtaaagtct gcgttagaaa
     1441 aattaaatgc gcatgtagca cgggtcgaat taattcatac cattaactac aacgatacct
     1501 ggatgcgtga ctacgggcct ttatcctgta ataccggcta tattcaattt gaatttaatg
     1561 g
//
LOCUS       DAOQGS010000805         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_60044_length_1560_cov_3.780066, whole genome shotgun sequence.
ACCESSION   DAOQGS010000805 DAOQGS010000000
VERSION     DAOQGS010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60044_length_1560_cov_3.780066"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            515..>1560
                     /locus_tag="PKZ52_18995"
     CDS             515..>1560
                     /locus_tag="PKZ52_18995"
                     /inference="COORDINATES: protein motif:HMM:NF020068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNG61915.1"
                     /translation="MWADAVSGEPKARPKLTIYEYQITGNTLLDPRDIERVLEPFLGE
                     NQEVSAVEKAAKKIERLYKDKGYPTVYVNIPEQDVRGGVIKMSVVEGKVQRLRITGAN
                     YYTLSGIRNELPAVQEGKTPYFPDVQQQLSKINARSQHLKVTPVLKPGKFPGSVDIEL
                     KVKDELPWVNQLSLSNFNSANTTRPRLEFNTAYNNLWQKDHGLSLQYQTSPEKTEEVS
                     IWGFTYLMPVMEQHRAAVYVVKSDSQVASLGGQTAFTALGKGDIYGLRYIIPLESHSD
                     FLHSVTLDFDYKDFTDTLEIAGNKAKGQADLRQDLAPIAYGLFGFDYDMTFMRAKGSD
                     RFGVGGRWGMRGLN"
BASE COUNT          367 a          345 c          420 g          428 t
ORIGIN      
        1 gactgcgcgg ttgcggcggt tggcagtggc actgatggct tcgatgaccg gggcgatttt
       61 catgaagttg gggcgggcgc cgacgacgtg aataattttg ctcatgtttt tgccttactt
      121 cttacgcgga ctgttgaggc tatggtgcct ctgctcagtg acagtcccgt ggcagtctgt
      181 tgacagtatt gttacagctg gccagagcca cactgccttt gcataaaaat gtcacaaaag
      241 tgtcacgctt gttttatcac tgccgccttt gcgtttcgtc acccagttgt cataaatcgc
      301 agtgacaatc tgcgcccgct tgtgtgcgct tcttcataat gtcttttgat tggcccgagc
      361 gcgccactgt ttgtgagatt ttaatctatg gctgtgtgag cccttttttt gtttatttac
      421 cgattgtttg tgcattcatt aacctcaacg gctgcccatg gtcttgctta aaaaaaattt
      481 tgttctattc gctttattaa atgtggcagc gcctgtgtgg gccgatgccg tgtcgggtga
      541 gcccaaggct cgccctaagc tgacaattta tgaatatcaa attaccggta ataccctgtt
      601 agacccgcgc gatatagagc gggtgttgga gcctttttta ggtgagaacc aagaggttag
      661 tgcagttgag aaagcggcta aaaaaattga gcgcttgtat aaggacaagg gttaccccac
      721 ggtgtatgtg aatattcctg agcaggatgt gcgcggcggt gtgattaaaa tgtccgtggt
      781 ggaaggcaag gtgcagcggc tgcggattac cggcgctaac tattacaccc tcagcggtat
      841 acgcaacgag ttgcccgccg tgcaagaagg caaaacgcca tatttccccg atgttcagca
      901 acagctgagt aaaattaatg cccgcagcca acacttaaaa gttacccctg tattaaagcc
      961 gggcaagttc cctggcagtg tggatattga attaaaggta aaggatgaac tgccttgggt
     1021 gaaccaatta tcgctgagca attttaattc ggcgaacacc acccggcctc ggttggaatt
     1081 taataccgcc tataacaatt tatggcagaa ggatcacggc ctctctttgc agtaccaaac
     1141 cagcccggaa aaaactgaag aagtgagtat ttgggggttt acctatttaa tgcctgtaat
     1201 ggagcaacac cgcgccgctg tatatgtggt gaaaagtgat agccaggtgg caagcctcgg
     1261 cgggcaaact gcatttacgg cgctgggtaa aggcgatatt tacggcttgc ggtatatcat
     1321 tccgctggaa tcccacagcg attttttgca ttcagtcacg ctcgatttcg attacaagga
     1381 ttttaccgat acgctggaaa ttgccggtaa taaagcgaaa ggccaagcgg atttgcggca
     1441 ggatttagca cccattgcgt atgggttgtt tggctttgat tacgatatga cgtttatgcg
     1501 ggccaaaggc tccgaccgct ttggtgtggg tggccgctgg ggtatgcgcg gtttaaatga
//
LOCUS       DAOQGS010000806         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_60312_length_1556_cov_5.493005, whole genome shotgun sequence.
ACCESSION   DAOQGS010000806 DAOQGS010000000
VERSION     DAOQGS010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60312_length_1556_cov_5.493005"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..410)
                     /locus_tag="PKZ52_19000"
     CDS             complement(165..410)
                     /locus_tag="PKZ52_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61916.1"
                     /translation="MKDKINIEHGKSYAPEKKTSSNAELVGDYWRAWTEGGKYYYECD
                     VGHFATNFSTVEIQESDFELLKSGGLSNADLERKYLA"
     gene            complement(421..>1556)
                     /locus_tag="PKZ52_19005"
     CDS             complement(421..>1556)
                     /locus_tag="PKZ52_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61917.1"
                     /translation="IDNLVNLSLDGIISNPKSPNQTTPNTSSREAEVVVTAPKPATLG
                     VAVVDTLGDVAHFVKGVPDNQKAAANDFYTLLTGGPIGLVTSKAVEKGMTLLPDPVLQ
                     TIAKTSEKVTDTAGQAGTSVLVGVGFDKVKESQESGHEVTEDRVSGVGAVVVTVAGAA
                     TAGAAGVLITKAKTPKIKNKNEGNDNPGKEDSEMVGQHQVPVWTTKPEIETLLRNQNV
                     QLKDDWNVDRIYSDVLQVEKGHRPDPSEYLSADYIAHHLAEFDNGAVRFTSRAAVEKY
                     GTAGNNEAFILPKDQFVKMINESNGDLRIVEEKLALSKGYLGDKDTMIVEIKPADLKG
                     LKMPSGNEKGANPLWVPGGRTGGGVSEAVVDLTKTPFTEIILK"
BASE COUNT          384 a          413 c          283 g          476 t
ORIGIN      
        1 tgaaaaatcc ataacggcct cagctattcc tccattcgtg tatccaccag gaatcgactg
       61 cccatttgca ccaccttcac tcccggaggg aatgcgaata tttttaatgt tttcaggctt
      121 cactaaaaag atcatcgtct cggaaccgct ttagtagctc tgacttaagc caagtatttt
      181 ctttcaaggt cagcattgct caaaccccca gactttagta attcaaaatc gctctcctgt
      241 atttccacag ttgaaaaatt ggttgcaaaa tgcccaacat cgcattcgta ataatattta
      301 ccgccctcgg tccatgctcg ccagtaatca cctactaact cagcattgct gcttgtcttt
      361 ttttcaggag cataactttt tccatgctcg atattgattt tatctttcat atagatagac
      421 tcacttaaga ataatttccg taaacggagt tttagttaaa tccaccaccg cctcggacac
      481 tcctccacct gtacgccctc ctggaaccca taaaggattg gctccctttt cgttgccaga
      541 tggcattttt aaacccttta gatcagcagg ctttatttcc actatcatcg tgtctttgtc
      601 acccagatac cctttgctta aagccagctt ttcttcaaca atacgtaggt ctccattaga
      661 ttcatttatc attttaacaa attgatcttt tggcaaaata aatgcctcat tgttaccagc
      721 tgttccatac ttttctaccg ctgctctgga agtaaatcgc acagccccat tatcaaactc
      781 agctaaatgg tgcgcaatat agtcagcgga taagtactca gacggatccg gtcggtggcc
      841 cttctcaacc tgaagaacgt cgctataaat tcgatcaaca ttccaatcgt cttttaactg
      901 gacattctga tttcttaaga gcgtttcaat ttcaggtttg gtagtccata ccggcacttg
      961 atgctgccct accatttccg aatcttcctt cccaggatta tcattgcctt catttttatt
     1021 ttttatttta ggtgttttag ccttagtaat taacacccca gccgctccgg ctgtagcagc
     1081 ccccgcgact gtaaccacca cagcgcccac accactcacg cggtcttctg ttacttcatg
     1141 acctgattcc tgagactcct tcaccttgtc aaaaccaaca ccgaccaaga cactggttcc
     1201 cgcttggcct gcagtatcgg ttactttttc actggttttg gcgatagttt gcaggacagg
     1261 atccggtaga agtgtcatgc ctttttcaac ggctttggac gtgactaacc cgatagggcc
     1321 gcctgtaagc aaagtataaa aatcgttggc agccgctttt tggttgtccg gcacgccttt
     1381 aacaaaatga gcaacatcgc ccaacgtatc gaccacggcc acacctaagg ttgcaggctt
     1441 gggcgcggtc acaaccactt cagcctctcg gctagatgta tttggagttg tttgattggg
     1501 cgattttgga ttgctaataa ttccgtcaag actcaggtta accagattgt ctatgg
//
LOCUS       DAOQGS010000807         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_60623_length_1552_cov_2.857715, whole genome shotgun sequence.
ACCESSION   DAOQGS010000807 DAOQGS010000000
VERSION     DAOQGS010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60623_length_1552_cov_2.857715"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKZ52_19010"
     CDS             complement(<1..258)
                     /locus_tag="PKZ52_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNG61918.1"
                     /translation="MKRVVITGMGIVSCIGHDLATVLSALRNGTSGIRYNPSYAEQGF
                     RSLVSGSIDLDLAAHIDRKQLRFMGDAAAFAYIAMQRAIEHS"
     gene            complement(295..819)
                     /gene="fabA"
                     /locus_tag="PKZ52_19015"
     CDS             complement(295..819)
                     /gene="fabA"
                     /locus_tag="PKZ52_19015"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HNG61919.1"
                     /translation="MHSFEPKASYTREELITCGNGQMFGPGNAQLPVPNMLMLDRIAH
                     ISQVGGEFGKGEIVAELDINPELWFFQCHFPGDPVMPGCLGLDAMWQLVGFFLAWKGN
                     PGRGRALGAGEVKFTGQILPSAKQVTYHINLKRVIERKLIMGIADGRVSVDGKDIYFA
                     KDLRVGLFGNTDSF"
     gene            1021..>1552
                     /locus_tag="PKZ52_19020"
     CDS             1021..>1552
                     /locus_tag="PKZ52_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNG61920.1"
                     /translation="MIKKCIFPVAGYGTRFLPATKSMPKEMLPVVNKPLVQYGVEEAL
                     EAGLTEIGFVTGRGKRAIEDHFDVSYELEHQIAGTDKEAYLTSIREVMAKCTFSFTRQ
                     REMRGLGDAILNSRRLVGDEPFAVVLSDDLCVKDGEPDGVLAQMVGLFKQFRCSIVAI
                     QEVPEDQIHKFGVIGGE"
BASE COUNT          364 a          400 c          429 g          359 t
ORIGIN      
        1 ggagtgttcg atggcgcgct gcatggcgat gtatgcaaag gcggcggcgt cacccataaa
       61 gcgcaattgt ttgcggtcta tgtgtgcagc aagatccaaa tcgatggacc ctgaaaccag
      121 gctgcgaaaa ccctgctcgg cataggaggg gttatagcga atacctgagg tgccattgcg
      181 cagggcgctt aatacggttg ccagatcatg accaatacag gaaacgatac ccatgccggt
      241 aattacaacg cgtttcatat ttgtcctctt gccttcgggc gtaaatagat ggtgctagaa
      301 agaatcggta ttgccgaaca gtcctacgcg cagatctttg gcgaaataaa tgtccttgcc
      361 atccaccgag acgcggccat ctgcaatgcc cataattaat ttgcgctcga tgacccgttt
      421 aaggttgata tggtaggtca cctgtttggc gctgggcaaa atctgcccgg taaattttac
      481 ttcgccagcc cccagtgcgc gcccgcgccc ggggttgcct ttccaggcga gaaaaaagcc
      541 caccagctgc cacatggcat caaggcccaa gcagccgggc ataaccgggt cgccggggaa
      601 gtggcactga aaaaaccaca gctcagggtt gatatccagc tctgccacaa tttcaccctt
      661 gccaaactcg ccgcctactt ggctgatatg cgcgatacgg tctagcatca acatgtttgg
      721 caccggcagc tgtgcattgc cagggccgaa catctggccg ttgccgcagg tgataagttc
      781 ctcgcgggtg taggaggctt tgggttcaaa tgagtgcata aaagactcta ggcagttaga
      841 cgaatgcccc taaaaactgg ggccctggcg aaaaggcctg ccattctacc tgcaaacccc
      901 gtgatgtcca gtcttagccg ggtggggata attcatgggc tgtgctatat tcgcgcgcct
      961 cgtttttgct gcacaccatc actcttcttt ttattgttaa ccacgttgta agggctcctt
     1021 atgatcaaga aatgtatttt ccccgtggcc ggttacggta ctcggttcct tcccgccacc
     1081 aaatccatgc ccaaagaaat gctcccagtg gtgaacaaac ccctggtgca atacggtgta
     1141 gaagaagcgc tagaagcggg tttaacggaa attggtttcg tgactggccg cggcaaacgc
     1201 gccattgaag accatttcga cgtgagctac gagctggagc atcaaattgc cggcacggac
     1261 aaagaagcct acttaacgtc tatccgcgaa gttatggcca agtgtacgtt ttcctttacc
     1321 cgccagcggg aaatgcgcgg cctgggcgat gcgattttaa atagccgccg cctggtgggc
     1381 gatgagcctt ttgcggtggt gttgtcggat gacctgtgtg taaaagatgg cgaacccgat
     1441 ggtgtgctgg cgcaaatggt ggggctgttc aagcaatttc gctgcagcat cgtggccatt
     1501 caagaagtgc cggaagatca aattcataaa tttggtgtga tcggcggcga gc
//
LOCUS       DAOQGS010000808         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_60811_length_1549_cov_4.627845, whole genome shotgun sequence.
ACCESSION   DAOQGS010000808 DAOQGS010000000
VERSION     DAOQGS010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60811_length_1549_cov_4.627845"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1247)
                     /gene="hemB"
                     /locus_tag="PKZ52_19025"
     CDS             complement(252..1247)
                     /gene="hemB"
                     /locus_tag="PKZ52_19025"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNG61921.1"
                     /translation="MHRSFPTTRMRRNRFAEFSRRLVRETELSAGDFIYPMFLIEGKN
                     RRETITSMPGIERFSLDLLAQEVETLLELRIPAIALFPNISQEKKTLDGKEAFNPDGL
                     IPTAVRFLKERYPQMGIICDGALDPYTTHGQDGIIDDTGYVLNDETVAALEQQACVCA
                     DSGADVIAPSDMMDGRVGAIRKQLDAKGFINTQIMAYSAKYASSYYGPFRDAVGSAGN
                     LGKSNKYNYQMDPANSDEALQEIALDLQEGADMVMVKPAMPYLDIVRRVKETFGVPTF
                     AYQVSGEYTMHKLAMQHGYLAEAVMLESLLCIKRAGANGILTYFAKDAARALQNQ"
     gene            complement(1345..>1549)
                     /locus_tag="PKZ52_19030"
     CDS             complement(1345..>1549)
                     /locus_tag="PKZ52_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61922.1"
                     /translation="IVQQAGLHSDTYEIGDIYRTIASLLPSMDGFQAYICGAPSFVKK
                     LKKQCFLAGANLNDIFADAFLPS"
BASE COUNT          389 a          373 c          377 g          410 t
ORIGIN      
        1 ccgtgaatac atccatgtag gctcgtcaaa aaggtccctc tttttgacgg tctgccagag
       61 cctgtattca ttgccgctac accgagaacc aattaccctg tagggcttaa ttttcagtaa
      121 ttatttagta ggctaaaact tatatatgcc tgcttttttc ctcgttccca cgctctgctt
      181 gccaacgagg ttaggaacga ggttaggaac gaggctagag gttacaattg ctcaatagct
      241 aaaaaccatt tttattggtt ttgtaacgcc cgcgcagcgt ccttagcaaa ataagtcaaa
      301 ataccattgg caccggcgcg cttgatgcac aataaggatt ccaacatcac cgcttcggct
      361 aaatacccgt gctgcatggc taatttgtgc atggtgtatt caccactcac ctggtaggca
      421 aaagtcggca caccaaaggt ttcttttact cgccgcacta tatccaaata aggcatagcg
      481 ggttttacca tcaccatatc ggcgccttct tgtaaatcca aggcaatttc ctgcagggct
      541 tcgtcggaat tagctgggtc catttggtaa ttgtatttat tgcttttacc taagttgcca
      601 gctgaaccca ccgcatcgcg aaatggcccg taataactcg acgcatattt agcgctgtaa
      661 gccatgattt gtgtattaat aaaacccttg gcatctaatt gtttgcgaat ggcacccacg
      721 cggccgtcca tcatatccga aggggcgatt acatccgcgc cgctatctgc gcacacacag
      781 gcttgttgtt ccaatgccgc cacggtttca tcatttaata cataacctgt gtcatcaata
      841 atcccgtctt ggccgtgggt ggtgtagggg tctagggcgc cgtcgcaaat tatgcccatt
      901 tgcgggtaac gctcttttaa aaatcgcacg gcagtaggaa ttaaaccatc cgggttaaag
      961 gcctctttac cgtcgagagt ttttttctcc tggctgatat tggggaataa ggctatggca
     1021 gggatgcgca attccaacag ggtttccact tcttgggcca ataaatccag gctaaagcgc
     1081 tcgatgccgg gcatggaggt aatagtttcc ctgcgattct tgccctcaat taaaaacatg
     1141 ggataaataa aatcccccgc gctaagctcg gtttcccgca ccaagcggcg ggagaattca
     1201 gcaaagcgat tgcggcgcat acgggtggtg ggaaaactgc ggtgcatggg ggctccaggt
     1261 tttagtgggg aaagcgcgca agactacaaa ggcttgcgct ttggggcaac ttaaaaaagg
     1321 ggcaaggcgt acttaaggct ggatttagct gggcaagaag gcgtcggcaa aaatatcgtt
     1381 taaattggcg cctgcaagaa aacactgttt ttttagcttt ttaacgaaac ttggtgcgcc
     1441 gcaaatatag gcctgaaagc cgtccatact cggcaagagg cttgcgatgg tgcggtaaat
     1501 gtcgcctatt tcataagtgt cactgtgcag gcccgcctgc tggactatg
//
LOCUS       DAOQGS010000809         1548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_60891_length_1548_cov_4.945077, whole genome shotgun sequence.
ACCESSION   DAOQGS010000809 DAOQGS010000000
VERSION     DAOQGS010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60891_length_1548_cov_4.945077"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKZ52_19035"
     CDS             <1..742
                     /locus_tag="PKZ52_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNG61923.1"
                     /translation="DYPTHAANKAVAIIPNCAATRHTHFTLDGSGPALQTPPALDDWP
                     EISWEVGESVRRVDVNTVTRADIASWKTGETLLLSGKILTGRDAAHKRLTDMIAKGEP
                     LPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRTVLEQTGLMGMIGKSERG
                     PTAISAIADNKAVYLMAVGGAAYLVSKAIKAAKVVAFADLGMEAIYEFDVQDMPVTVA
                     VDTSGESVHITGPKIWQAKIHELALKLS"
BASE COUNT          350 a          401 c          412 g          385 t
ORIGIN      
        1 agattacccc acccatgcgg ccaataaagc cgtcgccatc atccccaatt gcgccgccac
       61 ccgccacacc catttcaccc tcgatggcag cggcccggcg ctgcaaacgc cgcccgcctt
      121 agacgactgg ccagaaatta gctgggaagt aggcgagagc gtgcgccggg tcgatgtgaa
      181 tacagttacc cgtgccgata ttgccagctg gaaaaccggt gaaacactat tgctctcggg
      241 caaaatcctc accgggcggg atgcggccca taagcgcctc accgatatga ttgccaaggg
      301 cgagccgctg ccggtggact tcactaaccg ttttatttat tacgtgggcc cggtagatcc
      361 ggtgcgcgac gaagtcgttg gccctgccgg gcccaccacg gctactcgta tggataagtt
      421 cacccgcacg gtgctcgagc aaaccgggct tatgggcatg attggtaaat cagagcgggg
      481 gcctacggcg atttcagcga ttgccgacaa taaagcggtt tatttaatgg cagttggcgg
      541 tgcggcctat ctggtgtcca aggcaattaa agcagcgaag gtggtcgcgt ttgcggacct
      601 gggtatggaa gccatttatg aatttgatgt gcaggatatg ccggtaacgg tagcggtgga
      661 taccagtggt gagtcggtgc acatcactgg gcctaaaata tggcaggcga agattcacga
      721 actggctctt aagttgtcct agcctgtgcg tcgtttttgg caaggatgcc tggcggctag
      781 agttccttga atgcatcttt agcaccctca tgccgattaa aaattgccgt atttcttaag
      841 tatcttttgg gtgtaactag tcgtgattct cgcaccgact agaaatcatc agggagtgct
      901 tcgatgctgt ttgcccccgc agtggggaat acaggttatt gcaggcacgc cttgaataca
      961 tccgtgtagg ctcgtcaaca agatccctct ttttgacggt ctgataacct gtatttacca
     1021 ctgctgcaca gcaaatcaac caagctaaaa actgcagtag agtttaactg cggttttagg
     1081 attattcgac tcggctaaat cccctcgatt taccgcccgc gttgagaggc taatatggtg
     1141 tgctgaataa ttgccttcct tattactgcg cgtcgatttc tcgccctggg tgccgtatgt
     1201 gctcaattgc ggggtaaaaa cttatcttaa tcaacgcagg cagtgtgcac taacccgcat
     1261 gtacaaaatt taaccgcaga ctcattgcgt atattggttc gcagtggtct actgtcacct
     1321 aaattatcct ggtgggcttg gtcccgggcc tagggcagga gcgcaaacaa cctgttgcac
     1381 tcttaccttg ctaggcccgc aaagttaatt gtgctcaaga acgctccatc gcttggtcta
     1441 accccagcag ttttttagca catttgtgtg tcagttcggc gccgtctggt tggttcaacc
     1501 taaaaaccgc agttgaactc gtagcgaggg tgcctaaggc gctgaata
//
LOCUS       DAOQGS010000810         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_60969_length_1547_cov_4.318365, whole genome shotgun sequence.
ACCESSION   DAOQGS010000810 DAOQGS010000000
VERSION     DAOQGS010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60969_length_1547_cov_4.318365"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKZ52_19040"
     CDS             <1..656
                     /locus_tag="PKZ52_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61924.1"
                     /translation="SVVLYLPFGRAGALALAVPLNKWPLITRLMAAWLVMAVVFYSGG
                     AFLLQQALPLVQWIANSLSDNYTAQLSLGSSEQGAIIQIAATVSRNIYYATLPIAPKG
                     AVLTGAGTLVHTLTPLVILFSLLIAWPCPWRVLALRLVIGALLGLLIILGTLPFLLIS
                     HIEGTLHAAVQQFLPNPVPMPKVFYWVMFVEMGGLWLLPILAAMGSIALTQRLVLKR"
     gene            1273..>1547
                     /locus_tag="PKZ52_19045"
     CDS             1273..>1547
                     /locus_tag="PKZ52_19045"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNG61925.1"
                     /translation="MFIQSIQWRAVIAVFAALCCSQHAQAASPDLVISQVYGGGGSAG
                     APYSHDFIEVFNRGTAPVNLNGWSLQYASAAGNSWTNKTLLGNTLLAP"
BASE COUNT          332 a          372 c          414 g          429 t
ORIGIN      
        1 gctcagttgt tttgtatttg ccatttgggc gcgccggagc cctcgccctc gccgtgccgc
       61 taaataaatg gccactgata acgcgtttga tggcggcttg gttggtgatg gcggtggtgt
      121 tttacagcgg tggtgcattt ttgctgcagc aggcgctgcc gctggtacag tggatcgcca
      181 atagcctgag cgataattac accgcccaat tgagcttggg ttcttctgag cagggcgcga
      241 ttattcaaat tgccgccact gtttcccgca atatttatta tgcaactcta ccgattgcac
      301 ctaagggtgc ggtattaacc ggtgcgggca ctctggtgca tacactgacg cccttggtga
      361 ttttatttag tttgctcatc gcctggccct gcccctggcg ggttttagcc ctgcgcttgg
      421 ttattggtgc gctattgggc ctgctgatta ttcttggcac cctgccgttt ttattaatca
      481 gccatattga aggcaccttg cacgcagcgg tgcaacagtt cctgcccaat ccagtgccca
      541 tgccgaaggt tttttattgg gtgatgtttg tggaaatggg tgggctttgg ttgctgccta
      601 ttttggcggc catgggttcc attgcactta cccaaaggct tgtgcttaag cgttaaaact
      661 aaaaaaccag attgcggctg gttgcgtgga atgggagcga tctagcgtgc ataaattaat
      721 tgcgatgttt ttggtgtccg gctgagtaac aactgcttta ggtttaaaaa gtaactattc
      781 agcacatttt tacccgctcc atgcagacgg taaattgagg tggttttagc cgtgcggcac
      841 aaacgattcg cggtgtagca gtggtgaata aagcttgttg ccgaccgtca aaaaaaggga
      901 cctttttgat ggggctacac ggatgtattc acggcgtgtc tgcaacaagc tgtatttatc
      961 actgcggcgg ctcgcagcac gcaagcactc tcctgatggt tgcttgtgga cgcaagaaca
     1021 ctgcagctaa agtcaataag tactggcctg atcaaaaggt gctgactagt gacattcaaa
     1081 atgaatgtca cacagtgcaa aattctgcag caaatacttc atctaaacga cacggtttcg
     1141 acatttaaac tcggtatttt ctcagctatt aaataagcta acaggccgct taaatcgcgg
     1201 cagctgtttg cgggggtatt aattagcgtt aattaatgcc cctttggggt ttttggccga
     1261 ggaagcatca ctatgtttat tcaatccatc caatggcggg ctgttattgc tgtgttcgcg
     1321 gccctctgtt gtagccagca cgcccaggcg gcaagcccgg atttggttat cagccaggtc
     1381 tatggcggcg gtggcagtgc cggtgcgccc tatagccacg acttcattga agtgtttaac
     1441 cgcggcaccg cgccggtgaa tttaaacggc tggtccctgc aatatgcctc cgccgcaggc
     1501 aacagctgga ccaataaaac cctgctgggc aatacgctgc tggcccc
//
LOCUS       DAOQGS010000811         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_61535_length_1539_cov_5.173181, whole genome shotgun sequence.
ACCESSION   DAOQGS010000811 DAOQGS010000000
VERSION     DAOQGS010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61535_length_1539_cov_5.173181"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PKZ52_19050"
     CDS             <1..645
                     /locus_tag="PKZ52_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007645775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNG61926.1"
                     /translation="VLQPSARTGEYLFWQDTQSSLRRLGLGVLAAASLGLLVGLLTGA
                     LPMLGAGLTPFVTAISLIPPMAVLPILFIVFGLDELSKVMLIVIGIAPTLVRSVYQNC
                     RELPAEQWVKAQTLGANSGQVMLRVLLPQLTPRLIDAVRLSLGSAWLFLIAAEAIASN
                     DGLGYRIFLVRRYMAMDVILPYVAWITLLAFLTDYALARLNRWAFPWYNPASRH"
     gene            648..1490
                     /locus_tag="PKZ52_19055"
     CDS             648..1490
                     /locus_tag="PKZ52_19055"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG61927.1"
                     /translation="MSAAEPLLRFHRVGKEYGQHIVLEGISTQVQEGEFVSLVGTSGC
                     GKSTFLKMLLGTEQPTSGEAFLGAKPLATEPGPDRGIVFQKYSVFPHLTVQQNVLAAL
                     EFSRSPWLGKLFGGAKKAALEEVRHWLDAVGLSRAKDLYPHSLSGGMQQRLAIAQALI
                     KQPRILLLDEPFGALDPGIRKDMHQLLLRLWSEHKLTVFMVTHDLQEGFYLGTRLWVF
                     DKTRHDPQAPDRYGSTITYDLTLHSNNRQAVQEIADQLDAADVARMELSPAVVNTPTG
                     EPHV"
BASE COUNT          301 a          457 c          445 g          336 t
ORIGIN      
        1 gtgctgcagc cgagcgcccg caccggcgag tatttatttt ggcaagacac ccaaagcagc
       61 ctgcgccgct tgggcctggg ggttttggcg gcggcgtctt taggcttgtt ggtgggatta
      121 ctcaccggcg ccctgcccat gctgggggcg ggcctaacac catttgtgac ggcaatttcc
      181 ctgattcccc ccatggcggt gctgccgatt ttatttattg tgtttggctt ggatgagtta
      241 tccaaagtca tgctgattgt gattggcata gcgccgacct tggtgcgcag cgtctatcaa
      301 aactgccgag aattgcccgc cgagcagtgg gtaaaggcgc aaaccctggg ggctaattcc
      361 ggccaggtga tgctgcgggt gttactgccc cagttgacgc cgaggctgat tgacgctgtg
      421 cgcctaagcc tcggcagcgc ctggctgttt ttaattgcgg cagaagccat tgcctccaac
      481 gatggcttgg gctaccggat tttcctggtg cgccgttata tggctatgga tgtgatcctg
      541 ccctatgtgg cctggattac ccttttggcg tttttgaccg actacgccct ggcgcgctta
      601 aaccgctggg ccttcccttg gtacaacccg gcaagtaggc actaattatg agcgcagcag
      661 aacccctgtt acggtttcac cgcgtaggca aagaatacgg ccaacacatt gtgctggagg
      721 gcatttccac ccaggtgcag gaaggggaat ttgtctccct ggtgggcacc tccggttgcg
      781 gtaaaagcac cttcttaaaa atgctgctcg gcaccgagca gcccaccagt ggcgaggcct
      841 ttttaggcgc taagcccctg gctacagaac cggggccaga ccggggcata gtgtttcaaa
      901 agtattccgt cttcccccac ctcacggtgc agcaaaacgt cctggcggca ctggaattta
      961 gccgcagccc ttggcttggc aagctgtttg gcggggcgaa aaaagccgct ttagaagaag
     1021 tgaggcactg gctggacgca gtgggcctaa gccgggcgaa ggatctttac ccccacagcc
     1081 tctccggcgg catgcagcag cgcttagcca ttgcccaggc gctgatcaaa cagccgcgca
     1141 ttctgctgtt ggatgaaccc ttcggcgctt tggaccccgg cattcgcaag gacatgcacc
     1201 aactgctgct gcggctgtgg agcgagcata agctgacggt gtttatggtc acccacgacc
     1261 tacaggaggg gttttatttg ggcacccgcc tgtgggtgtt cgataaaacc cgccacgacc
     1321 cccaggcgcc ggatcgctac ggcagcacca tcacctacga tctcaccctg cattccaata
     1381 accgccaagc ggtgcaggaa attgccgacc aattggacgc tgccgatgta gcccgtatgg
     1441 agctaagccc cgcagtcgtc aacaccccca caggagaacc ccatgtttga ggcgcgcccc
     1501 aacaccgccc ccagtgccca gggctacagc acttttttg
//
LOCUS       DAOQGS010000812         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_61856_length_1535_cov_3.910811, whole genome shotgun sequence.
ACCESSION   DAOQGS010000812 DAOQGS010000000
VERSION     DAOQGS010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61856_length_1535_cov_3.910811"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKZ52_19060"
     CDS             complement(<1..622)
                     /locus_tag="PKZ52_19060"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNG61928.1"
                     /translation="MTKLPETLRENVRLLGELLGETLLKHEGEAVFSKVEEIRKLGKA
                     INKASEGDSQQLVDLLDKVGDAEILPIVRAFNQFLNLANLAEQEYASSTEAASVDTLD
                     HLLGEQKQRLNEAEFLEVIRNLSIDLVLTAHPTEITRRTLIRKYDQITSVMAGHRRTD
                     LLAYEHDKYRGRLKRLVEEIWATDEIRNERPTAVDEARWGFAVIESS"
     gene            complement(810..>1535)
                     /locus_tag="PKZ52_19065"
     CDS             complement(810..>1535)
                     /locus_tag="PKZ52_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNG61929.1"
                     /translation="GGLQSQPKMHFPGGLLIGDDAGTLNFAKIKGSHTAMKSGMIAAE
                     VLAKALAQGLEATDLSEFAAAYEDSWAYDELYLQRNFGPAQHRWGNLWGSAYAFVDIN
                     LFGGHLPWTLSDNKADHACLQPAADCPVIQYPKPDGVLSFDKLSSVYLSNTNHEEDQP
                     CHLRLLDPSIPVAKNLPLYAEPAQRYCPAGVYEIVEQEGAARLQINGQNCVHCKTCDI
                     KDPAQNITWVTPEGAGGPNYPNM"
BASE COUNT          293 a          426 c          448 g          368 t
ORIGIN      
        1 ggctgctttc aatgactgca aatccccagc gggcttcatc caccgctgtg gggcgctcgt
       61 tgcggatttc atcggtggcc caaatttctt ccactaaccg ctttaggcgg ccacggtact
      121 tgtcgtgctc ataggcgagc aggtcggtgc ggcggtggcc cgccatcacg ctggtaattt
      181 ggtcgtattt gcgaatcagg gtacgacggg tgatttccgt ggggtgggcg gtgagcacta
      241 ggtcgatgga gaggttgcgg attacctcta aaaattcagc ctcattcagc cgctgctttt
      301 gctcgcccag caggtggtca agggtgtcca cgctggcggc ttcggtgctg ctggcgtatt
      361 cctgctcggc gaggttggcc aggttcaaaa actgattaaa cgcccgcacg atgggtagaa
      421 tttccgcgtc ccccacctta tccagcaagt ccaccagttg ctgcgaatcc ccttcggagg
      481 ctttgttaat ggccttgccg agcttgcgaa tttcctccac cttggaaaat acggcctccc
      541 cttcatgctt taacagggtc tcccccagta actcgcccaa caaacgcacg ttttcgcgca
      601 gggtttcggg taatttggtc atctataact ctcctcaatg atggggcgca ttctatcatc
      661 gccgcgatta gttgccgcaa ttcccgtgcc gcttgttatt ttcgtatatt taaaaatggg
      721 atagctatca agcttcgctt tgcatagagc cacccgccgc ctcccatctg ggcggcgggt
      781 ggcccagcgc cagccacatt cagcgccagc tacatattgg ggtagttagg gccacctgcc
      841 ccttccgggg ttacccaggt gatgttctgg gccgggtctt tgatgtcgca ggttttgcag
      901 tgcacgcagt tctggccatt aatctgcaag cgcgccgcgc cctcctgctc aacaatttca
      961 tacacaccgg ccgggcagta gcgctgggca ggttctgcat ataaaggcag gttcttcgcc
     1021 accggaatac tcgggtcaag caagcgcagg tggcagggtt ggtcttcctc gtgattggtg
     1081 ttggagaggt ataccgagga gagcttgtca aaactcaaca caccgtctgg cttggggtac
     1141 tggataaccg ggcaatcggc ggcgggttga aggcaggcgt ggtcggcttt gttatcgctt
     1201 aaggtccagg gcaagtggcc gccgaacagg ttgatatcta caaaggcgta ggcagacccc
     1261 cacaaattgc cccagcggtg ctgggccggg ccgaaattgc gctgcaggta cagctcgtca
     1321 taggcccagg aatcttcata ggcggcggca aactcgctta agtccgtggc ttcaagccct
     1381 tgggcaaggg ctttagcgag cacttccgcg gcgatcatgc cggacttcat agccgtgtgg
     1441 gagcctttga ttttggcgaa attaagggtg cctgcgtcat cgccaattaa taggccgccg
     1501 gggaaatgca ttttcggctg agactgcaag ccgcc
//
LOCUS       DAOQGS010000813         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_61984_length_1533_cov_3.880244, whole genome shotgun sequence.
ACCESSION   DAOQGS010000813 DAOQGS010000000
VERSION     DAOQGS010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61984_length_1533_cov_3.880244"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..585)
                     /locus_tag="PKZ52_19070"
     CDS             complement(118..585)
                     /locus_tag="PKZ52_19070"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HNG61930.1"
                     /translation="MSITLPPCTLHTRPATHFAGLKIPVGERAAEEIPALWQTFMAQN
                     PGQFMQGDAEFGLCIAGASGTDYMAAVPLTEHAQVPAGWSHISLPATRFAVFEHLEPV
                     WRIRETIDAIFTPGALKHTHQPVNSIAFLECYSPEFNPQTGTGGMSIWVPITP"
     gene            complement(603..1028)
                     /locus_tag="PKZ52_19075"
     CDS             complement(603..1028)
                     /locus_tag="PKZ52_19075"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNG61931.1"
                     /translation="MKSLSPYIIFFGRTREALEFYQQALKGEVVNIMKFADGGSACAE
                     MAADPEGVMHSEFRAEDVHFFASDGIPGSSPINGNRITLAINFSHLDEQAAVFNALAE
                     GGEVTQPLADQFWGAKYGELIDKFGLHWSLNCFTAPAHT"
     gene            complement(1044..1433)
                     /locus_tag="PKZ52_19080"
     CDS             complement(1044..1433)
                     /locus_tag="PKZ52_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNG61932.1"
                     /translation="MKYLCLVYCDEQEFNRLSPSEWKTLNERCVECVASLQNSGHFIA
                     GDALHPVATATSVRLRDGKVLITDGPFAETKEQLAGYYLLEARDLNEALLLAQKIPAA
                     SMGCIEVRPVRTLDPTIDERINPIINR"
BASE COUNT          361 a          359 c          430 g          383 t
ORIGIN      
        1 ggcacttcga tgccgggttg ttagagaggg ggagtccatc tcattgacct acgctcgaac
       61 gggcttttcc cttaatttaa tgacattgcc ctgcgaaggg gaaactgcgt tgttttgtta
      121 aggtgtaatc ggcacccaaa tactcatgcc ccctgtgcct gtttgcgggt taaattccgg
      181 cgaataacat tctaaaaaag caattgaatt gaccggttgg tgggtgtgtt ttaaggcgcc
      241 gggtgtaaat atggcgtcga ttgtttcgcg aatacgccag acaggttcaa ggtgctcaaa
      301 cacggcaaac cgagttgcgg gcaggctgat gtgcgaccag cctgcaggta cttgtgcatg
      361 ttcggttaag ggcacggcgg ccatatagtc cgtgccggag gcgcctgcga tacacaggcc
      421 aaattcagca tcaccctgca taaattgccc tgggttttgc gccataaagg tttgccaaag
      481 ggcgggaatt tcctctgcgg cgcgctcgcc cacgggaatt tttaagcccg caaagtgtgt
      541 tgctgggcga gtgtggagtg tgcagggcgg tagggtgata ctcatgggcg aaattcctgt
      601 gtttaagtgt gcgctggagc ggtaaaacaa ttaagggacc aatgcaggcc gaatttatcg
      661 atcaactcgc cgtatttagc gccccagaat tgatcggcta ggggttgtgt tacttcgccg
      721 ccttcagcca gggcattaaa taccgccgct tgttcgtcca ggtgggaaaa attaatggcg
      781 agggtaatgc ggttgccatt gatggggctg ctgccgggaa tgccgtcgga ggcaaaaaaa
      841 tgcacatcct cggcgcgaaa ttcgctgtgc atcacgcctt cagggtcggc tgccatttcg
      901 gcgcaggcgc tgccgccatc ggcgaatttc ataatattga caacctcacc ttttagagcc
      961 tgttgataaa attccaatgc ctcacgggtg cggccgaaaa aaataatata gggggatagg
     1021 gatttcatac aggcaccttt tatttaacga ttgatgatgg ggttaatgcg ttcgtcgatg
     1081 gttgggtcta gggtgcgcac ggggcgcact tcgatacagc ccatactggc agcggggatt
     1141 ttttgtgcaa gcaatagcgc ttcgtttaaa tcccgcgcct ctaataaata atatccggcc
     1201 agttgttctt tggtttcggc aaacggcccg tcggtaatta ataccttgcc atcgcgcagg
     1261 cgcacgctgg tggcagtggc taccgggtgc aaagcatcgc cagcgataaa atggccgcta
     1321 ttttgaagac tggcgacaca ttccacacag cgctcattga gggttttcca ttcgctgggg
     1381 gataaacgat taaattcttg ttcgtcgcaa taaactaaac ataggtattt cataggctgc
     1441 cctccataca aaaaaattcc cacacatggc cgtcgagatc tttaaaactg tgctggtaca
     1501 taaagccgtg gtctatgggc tcagaaaagc agc
//
LOCUS       DAOQGS010000814         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_62246_length_1529_cov_5.368385, whole genome shotgun sequence.
ACCESSION   DAOQGS010000814 DAOQGS010000000
VERSION     DAOQGS010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62246_length_1529_cov_5.368385"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..>1529)
                     /locus_tag="PKZ52_19085"
     CDS             complement(40..>1529)
                     /locus_tag="PKZ52_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61933.1"
                     /translation="SSSSSSSSSGALVKTEVSVVIEFGAGKPALAQKIDATLTLLNSA
                     DQPIASLQPVAAADASQTKFSLNGELSAIDAVALVVDIRHEGFTRFARRLPIQDSVYV
                     LARLEALTETAATAGNSVSISGSTTNGFTLQVAQDNETAPSMSISIPASSLPAGTQSL
                     TAAIKTYDPNNAEDAQYFPGSYADSTGNNLVSVAFNYAEVKTNTGESLQTLAANAQAL
                     RQARGALAFAAPPEPVIINRTIPASSCATLEKLGDANAELAGFQIPVYTYNPRSGLWD
                     LLGYGTVYTQAGVAIPGDTKDLDCAGTTYVLEIKVTNEIFLSDWWNLDYPLQFNEPIK
                     RCANLHIVNESNEPLAGVHGLITGDSNFASEYFVTDDSGNVSLSLEVFADDVSTGSFI
                     IWGANATAGNFNLSSVCPAPERQTVILKRPQLCKIKGHVSYAGKPLANLGVFAFNEAA
                     ILTDYWFSFGSTNSEGTYTLDVACDQPYTVEMYFAEENLYKNGSS"
BASE COUNT          364 a          379 c          395 g          391 t
ORIGIN      
        1 aggccatttt tccatctggc gcgggggttt tacacaattc tacgaagagc catttttata
       61 aaggttttct tctgcaaaat acatctcaac tgtgtagggt tgatcgcagg ccacatcgag
      121 cgtgtaggtg ccttcgctat tggttgagcc aaagctaaac caataatcgg ttaaaatggc
      181 tgcctcgtta aaagcaaaaa cccccaggtt agcaagcggc ttaccggcgt agctcacatg
      241 gccttttatt ttgcacaact gcgggcgttt taaaataact gtctgccgtt cgggggccgg
      301 gcaaacactg gataaattaa aattgcccgc agtagcattt gcaccccaaa taataaagga
      361 gcctgtgctc acgtcatcgg caaaaacctc aaggctgagg ctaacattgc cgctgtcatc
      421 agtaacaaaa tattcactgg caaaattgct atcgcccgta attaacccgt gtacaccagc
      481 caagggctca ttgctttcat tcacaatgtg taaattagcg cagcgtttta taggctcatt
      541 aaattgcagc gggtaatcca ggttccacca atcgcttaag aaaatttcat tggtaacctt
      601 aatttccagc acataggttg taccggcgca atcgaggtct tttgtatcac ctgggatggc
      661 aacacccgcc tgggtataca ccgtgccgta gcctaaaaga tcccacaagc ctgagcgcgg
      721 gttataggtg tacacaggaa tttgaaagcc cgctaattcc gcattggcgt cgccgagttt
      781 ttctagggtg gcgcagctgc tggcgggaat ggtgcggttg ataatcaccg gctctggggg
      841 cgctgcaaac gctagcgcac cgcgcgcctg gcgcagggcc tgggcgtttg cggccaaggt
      901 ttgcaagctc tcacctgtat tggttttaac ttcggcgtaa ttaaatgcta cagataccag
      961 gttattgccc gtggaatcgg cgtaactgcc ggggaaatat tgtgcgtctt cggcattgtt
     1021 ggggtcgtag gttttaatcg ccgcggtgag cgattgggta cccgcaggta ggctggatgc
     1081 cggaatactg atcgacatgc tgggcgccgt ttcattgtcc tgggcgacct gtagcgtaaa
     1141 gccgttggtg gtagagccgg aaatcgatac gctattgcca gctgttgccg ctgtttcggt
     1201 taaagcctct aaacgtgcta acacatacac gctgtcctga attggcagcc ttcgtgcaaa
     1261 acgggtgaat ccctcatggc gaatatccac taccagggca accgcatcta tggcactcaa
     1321 ttctccgttc aggctaaatt tagtttgact ggcatcggcc gctgcaactg gctgcaggct
     1381 ggctatgggt tggtcggcac tatttaacag cgttagggtg gcatcaattt tttgcgccag
     1441 cgcaggtttg cccgcgccaa attcaataac aacactcacc tcagttttta ccagagcacc
     1501 tgagctagac gagctgcttg aagaacttg
//
LOCUS       DAOQGS010000815         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_62898_length_1520_cov_4.894881, whole genome shotgun sequence.
ACCESSION   DAOQGS010000815 DAOQGS010000000
VERSION     DAOQGS010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62898_length_1520_cov_4.894881"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..927)
                     /locus_tag="PKZ52_19090"
     CDS             complement(112..927)
                     /locus_tag="PKZ52_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61934.1"
                     /translation="MPPIQPHKTIALLFHEQDSPATIHNYLITYYARIWQQLGFKVFA
                     LFGTHQFIPADLILVHVDLSVVPEAYCTFAARYPVVLNGRVRDIRKSAISQQLLKPGD
                     NYSGKVIVKSDCNYAALPEQRLHCQSATTFFSSPLDYHVFPNIHAVPDQLWQEPSLVV
                     EQFLPELIGGLYHVRFYNFLGSKGNCMRIASTHPIVHISHAVTIEQIPLDPRLDELRQ
                     AHHFDYGKFDYVERDGEIILFDTNKTTGFVGGSTDPKWQQARYERALGILDYL"
     gene            complement(1249..>1520)
                     /locus_tag="PKZ52_19095"
     CDS             complement(1249..>1520)
                     /locus_tag="PKZ52_19095"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNG61935.1"
                     /translation="QELLQRAIRVHGNFNEYVPLGLVLLLLVAANQAPNYLIHSLGAA
                     LLTGRLLHALGVSRSPEPLILRQVGMVLTTGSIITSAGFLLVHLV"
BASE COUNT          429 a          355 c          377 g          359 t
ORIGIN      
        1 taaatgagca tttttttggc aaattcaacg acgccagggc gagtaaggta attttcagag
       61 gttccttaaa tgcttgacgg cgtgaggaca ctaaaacgct gcgctcaata gttaaagata
      121 atcaagaatc cccaaagccc gctcatagcg cgcctgctgc cattttggat ctgtactgcc
      181 gcccacaaac ccagtggttt tattggtgtc aaataaaata atttccccat cccgttccac
      241 ataatcaaat ttaccatagt caaaatggtg cgcttggcgc agttcatcta agcgagggtc
      301 tagggggatt tgttctattg ttactgcatg gctaatgtgc actataggat gggtggaggc
      361 gatgcgcatg caattgcctt tgctgcctaa aaaattataa aagcgcacat ggtataaacc
      421 gccgattaat tccggcaaaa actgctccac caccaggctc ggctcctgcc acaactgatc
      481 aggcacggca tggatattcg ggaagacgtg gtaatccaaa gggctgctaa aaaatgttgt
      541 ggcgctctgg cagtgaaggc gttgctcggg cagggctgcg tagttgcaat cggatttgac
      601 aatcactttg ccggaataat tatcgccggg ctttagcaac tgctggctaa tcgcggattt
      661 gcggatatcc cgcaccctgc catttaacac cacggggtaa cgggcggcga acgtacaata
      721 ggcttctggc accaccgaga gatccacatg caccaggatt aaatccgccg ggataaattg
      781 gtgtgtacca aacagggcga acactttaaa acctaactgc tgccaaatac gggcgtagta
      841 ggttattaaa taattatgaa ttgtcgcagg ggagtcctgc tcgtgaaata acagggctat
      901 ggttttgtgg ggctggatag gaggcatagg ggaagattac gcgctaatgg ccatataccc
      961 ggctgttgtt tatccgcatt cactgggctt agcaaaacca cctgccctaa tccattaccc
     1021 catttaactg cagcgaatta aattcgggca aaaatagcaa aaaaacactt aaacctaaaa
     1081 accgcagttg aactcgtagc gagggtgcct aagacgctga atatgggcgc ttttttgtca
     1141 aaatttggcg aaagcagtag tcacactacg gtgagtcaaa tttttacaaa aaagcgggca
     1201 gattcaagta ttaggcagcc gtagtagagt tcaactgcgg cttttaggtt aaaccaaatg
     1261 cactagtaaa aacccagcgc tggtgataat actgccggta gttaacacca tgcccacttg
     1321 gcgcaaaatt aatggctcgg ggctgcggct gacgcccagt gcgtgcagca ggcggccggt
     1381 taacagcgca gcgcccaagc tatgaattaa ataattcggt gcttgattgg cggccaccag
     1441 caataaaagc acaagcccta aaggaacata ctcattaaag ttgccgtgca cgcggatggc
     1501 ccgctgcaat aactcctgct
//
LOCUS       DAOQGS010000816         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_63455_length_1513_cov_4.988340, whole genome shotgun sequence.
ACCESSION   DAOQGS010000816 DAOQGS010000000
VERSION     DAOQGS010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63455_length_1513_cov_4.988340"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKZ52_19100"
     CDS             <1..270
                     /locus_tag="PKZ52_19100"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNG61936.1"
                     /translation="QEKPFSLRLARSEKTLAVRGSQSVLEALESVGISPAFDCRVGNC
                     GQCAVKVLAGDVEHRDRVLSDADKNDGLMCACVSRPHGDTLVLDI"
     gene            368..979
                     /locus_tag="PKZ52_19105"
     CDS             368..979
                     /locus_tag="PKZ52_19105"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNG61937.1"
                     /translation="MLELFEFPLSGNCHKVRLMLSALKLGYKSTLLNGAAKEQKSAEF
                     LALNPFGQVPVLKDGNVIIRDSQAILVYLARAYGDDAWFPAEAAQAAAVTAWLSTAAN
                     EVVRGPNALRLHYKFGRAINREEASLITQNLLVLLNEHLAQHNWLATPKMSIADLAVY
                     PYIALAHEGQVDLAPFVHVKNWLSRIELFSWFVPMPGIQLQAH"
     gene            995..1471
                     /locus_tag="PKZ52_19110"
     CDS             995..1471
                     /locus_tag="PKZ52_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNG61938.1"
                     /translation="MTQQESRPPFPPFNRDSAMQKVRAAEDGWNNRNPQKVALAYTLD
                     SQWRNRDLFINGRSEIEQFLTQKWARELEYRLIKELWAHDNNRIAVRFAYEWHNDQGQ
                     WFRSYGNENWQFNEQGLMQKRYASINDLAIEEHQRKFTWPQGPRPLDYAGLSELGL"
BASE COUNT          372 a          358 c          388 g          395 t
ORIGIN      
        1 caagaaaaac cgttcagctt acgattggcg cgttcggaga aaaccctggc ggtacgcggt
       61 tcacagtcgg ttcttgaagc gctcgaatca gtgggcataa gccctgcctt tgattgccgg
      121 gtgggtaatt gtggccagtg tgcggttaag gttttagctg gcgatgtgga gcatcgcgat
      181 agggttttaa gtgatgcgga taaaaatgat ggcctaatgt gtgcctgcgt gtctcgccct
      241 cacggcgata ccctggtgct agatatatag gcaaatagcc aacagcgtgt ttttaacttt
      301 ccccattaat tattgaatcg gcctgcgcgc gggccaatac agtttaatct gcctaattgg
      361 agctattatg cttgagctat ttgaatttcc cctctccggc aattgccata aagtgcgttt
      421 gatgctgtcg gcattaaaac tgggctataa aagtactctg ctcaatgggg cagctaaaga
      481 gcaaaaatcc gctgaatttt tagcgctcaa tcccttcggg caggtgccgg ttttaaaaga
      541 tggcaatgtt attatccgcg atagccaagc catattggtt tatttggcgc gcgcctatgg
      601 ggatgatgct tggtttccgg ctgaggctgc gcaagcggct gcggttaccg cctggttatc
      661 caccgctgcc aatgaagtgg tgcgcggccc caatgcattg cgcttacatt ataaatttgg
      721 ccgagccatt aatcgtgaag aggcgagcct aattacccaa aacctattag ttctcttaaa
      781 tgagcattta gctcagcaca attggctagc cacacccaaa atgagcatcg ccgatttggc
      841 tgtgtacccc tatattgcat tagcccacga aggccaggtg gaccttgccc catttgtgca
      901 tgttaaaaac tggctgagcc gcatcgaact gttcagttgg ttcgttccca tgcccggcat
      961 ccaattacaa gcccactaac acttggagat agctatgacc cagcaagaat cccgtccgcc
     1021 ctttcctcct tttaaccgcg atagcgccat gcaaaaagtg cgcgccgccg aagacggttg
     1081 gaataatcgc aatccgcaaa aagtcgcgct ggcttatacg cttgatagcc aatggcgcaa
     1141 ccgcgattta tttatcaatg gccgcagtga aatagaacag tttttaacgc aaaaatgggc
     1201 gcgcgagctt gagtatcggt taattaaaga actctgggcc cacgataata atcgcattgc
     1261 cgtgcgcttt gcctatgaat ggcataacga tcaaggccaa tggttccgat cctacggcaa
     1321 tgaaaattgg cagtttaatg aacagggttt aatgcaaaag cgctatgcca gcattaatga
     1381 tttggcgatt gaggagcacc agcgaaaatt cacctggcca caggggccaa ggccccttga
     1441 ttatgccggg ttgagtgagt tgggattgtg agtggaagtt gcgcggcttt tctgtccttt
     1501 ttgctcattg gat
//
LOCUS       DAOQGS010000817         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_63637_length_1511_cov_4.183379, whole genome shotgun sequence.
ACCESSION   DAOQGS010000817 DAOQGS010000000
VERSION     DAOQGS010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63637_length_1511_cov_4.183379"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            670..>1511
                     /locus_tag="PKZ52_19115"
     CDS             670..>1511
                     /locus_tag="PKZ52_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNG61939.1"
                     /translation="MTLNRRRFLINSSLATALSPLASRVLADVRTGLKDTFKDRFLIG
                     TAVNADMTRGKYPDITEIIKRNFSSITPENALKWESLRTKEGEWKWAEADSFVAFGQE
                     HKIHSVGHCLAWHSQIPDSVFKNAKGSYIKPAQLRSKMTDHITTLVSRYKGKLAAWDV
                     VNEAVGDDNVMRKSHYYKILGEDFIDLAFNLAHEVDPSAHLLYNDYNIERDGKREAAL
                     ALLGRLKKRGVPVQGVGIQGHISIDGPSIADIEKSIEAYAKLGLRVHFTELDVDVLSR
                     EWESS"
BASE COUNT          435 a          366 c          353 g          357 t
ORIGIN      
        1 gccattgccg atggcagctt cgataaaatc ttttacagcg aacccaatat taaagatgtg
       61 ctggaaaaag ccaatatgaa aaaccgccgt atctttaacc tcaccaaccc attcctcacc
      121 aaagaaaccc ccttggatcg ggaagagctt tggtttgatc cgcaaaaagg tccataatcg
      181 ttaaatagca tgcgtttttt gatgggacat ttgagcaggc tcaggttagc gccgcgcagt
      241 caaccgcagg gaaattttta aagccgataa ttctgttttg tgctggtgct aggctctaag
      301 ttgagcaata aagtgcacca gcagtagtgc atactttgca aatcaagtgt aactattcag
      361 cacgctttat gatcttcttc acgcagtcag acaattgcgg agaatttaac aagacgccac
      421 acccctaaca tttaaggtaa ttgattcgcg gtgtggcagg ggtgaatccg cccactaaaa
      481 aaggcgctca aaaactacta aatagttact cgcaattaaa aatggcgatc taggccgcaa
      541 acaatagccc tcacgccatt tgcatttttt gtctaatatt agctgggtca aatccggcca
      601 acaggccaaa ccctttgtta ttccgctgta acacatccaa gcttcacatt cactatgggc
      661 agaaccctta tgacgcttaa tcgcagacgt tttcttatca attccagcct agccaccgcc
      721 ctaagtccgc tggccagtcg cgtattggcc gatgtgcgca ccggcttaaa agatacgttt
      781 aaagaccgtt ttttaattgg cacagcggtt aatgccgata tgacacgggg caaatatcct
      841 gatattacgg aaattatcaa acgcaacttt tcttccatca cgcccgagaa cgcacttaag
      901 tgggaaagct tgcgcactaa ggaaggcgag tggaaatggg cggaggccga cagttttgtc
      961 gccttcgggc aggagcacaa aatccacagc gtcggtcact gcctggcatg gcacagccaa
     1021 atccccgata gcgtatttaa aaatgccaag ggcagctata taaaacccgc tcaattgcgc
     1081 agcaaaatga ccgaccacat caccacattg gtaagccgtt acaagggcaa attagcggcc
     1141 tgggacgtgg taaacgaagc ggttggcgat gacaatgtga tgcgcaaaag ccattattat
     1201 aaaattttgg gagaagactt tatcgacctg gcctttaacc tcgcccacga agtagaccct
     1261 agtgcacacc tgctttacaa cgattacaac attgaacggg acggtaaacg ggaggcggcc
     1321 ctggcgctat tagggcgttt aaaaaaacgc ggggtgccgg tacagggtgt gggcattcaa
     1381 ggccatatat cgattgatgg cccaagtatc gccgatattg aaaaaagtat tgaagcctac
     1441 gcaaaattgg gcctgcgtgt gcattttacc gaactggacg tagacgtcct ctcgcgggag
     1501 tgggaatcct c
//
LOCUS       DAOQGS010000818         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_63813_length_1509_cov_4.892710, whole genome shotgun sequence.
ACCESSION   DAOQGS010000818 DAOQGS010000000
VERSION     DAOQGS010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63813_length_1509_cov_4.892710"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKZ52_19120"
     CDS             complement(<1..915)
                     /locus_tag="PKZ52_19120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNG61940.1"
                     /translation="MNYLMICGRLLACWLLALSCHAEDFAGLAKNLNAESFSEKSAAA
                     AALYQLDDPRVLPTLKAMQEGNLYWLSESSQPVIGKEVGDKLELTSCVEQTSLGLVDK
                     SQVEKIKINNGLRRDLRGFVAGLELSAKDPSIRQQSARNLIKNPDESLRAPLVAALDQ
                     EKNNSIKEIMRVALASIDMGSSDAALRKTAVATLAEALQPEAIALLEKASAKGENGQY
                     LHEDTEFAASSEAALKAMQAKVDLFKFLENCFFGISLGSVLLLAAIGLAITFGVMGVI
                     NMAHGEMIMLGAYTTFAIQQLFPQWMQAS"
     gene            complement(1147..>1509)
                     /locus_tag="PKZ52_19125"
     CDS             complement(1147..>1509)
                     /locus_tag="PKZ52_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HNG61941.1"
                     /translation="NDPMEATYIGFKMWATAVTKAGTTKVDDVREAIYGVAVPNLTGG
                     IAVMNTNHHLTKPVLIGEIQENGQFEIVWKTPGGVIGDAWTDFLPESANVVADWLAPI
                     KCGNFNIKTGKCSGQNYK"
BASE COUNT          395 a          386 c          342 g          386 t
ORIGIN      
        1 agatgcctgc atccactggg gaaatagttg ttggatcgca aacgtagtgt aagcgcccaa
       61 cataatcatt tccccatggg ccatattgat aacgcccatc accccaaagg taatagcaag
      121 gccgatagca gctagtaata acaccgagcc caaactaatc ccgaaaaaac aattttctaa
      181 gaatttaaat aaatcaacct tagcctgcat ggcttttaat gccgcctctg aactggcggc
      241 aaattcggta tcctcatgca gatactgacc gttttcgcct tttgcagagg ctttttccag
      301 cagggctatg gcctcaggtt gcagcgcttc agccagggta gcgacagccg tcttgcgcaa
      361 ggcagcatcg ctactgccca tatcaatact ggctagggca acacgcatga tctctttaat
      421 cgagttgttt ttttcctgat ccaaagcagc cactaagggg gcccgcaagc tttcatctgg
      481 atttttaatt aaattgcgcg ccgattgctg gcgaatactc gggtctttag cgctcagctc
      541 taggcccgca acaaagccgc gcaggtctcg gcgcaggccg ttgttaattt taattttttc
      601 cacctgggat ttatccacta gccccaagga agtttgttca acacaggaag ttaattccaa
      661 cttatcgccc acttccttac caataaccgg ttggctcgat tcactcagcc aatataaatt
      721 cccctcctgc atggctttta acgtgggcaa tacgcggggg tcgtctagct ggtataaggc
      781 agcggctgcc gctgattttt cagaaaaaga ttctgcattc agatttttag ccagcccggc
      841 aaaatcctcg gcatggcaac ttaacgccag cagccaacag gcaagcaagc gcccgcaaat
      901 catcaaatag ttcattatga gtccccaata gcctcgtcat actatgtata aagcagctgt
      961 acaggtaatt aagcgaatac gcgttgagag agaatcaatc ctgctgcaat gaaaagagcg
     1021 cttgggttaa taaaattaat cccggctcag ccgcgcatag aagcgggtta aatccatttg
     1081 acagatggct aaaaccatcg aagtagcacc acagcctgcg gctgcagtgc tactgggttc
     1141 gagcgattat ttatagtttt ggccggaaca tttgccagtt ttgatgttga agttaccgca
     1201 cttgataggc gccaaccaat cagccactac gttggcagat tcaggcaaaa agtcggtcca
     1261 agcatcaccg atcacgccgc cgggggtttt ccaaacgatt tcaaattggc cattttcttg
     1321 aatctcgcca attaatacag gtttggttaa gtggtggttg gtattcataa cggcaattcc
     1381 gcccgtcagg ttaggcacag ctacgccgta aatggcttcg cgcacatcat ccactttggt
     1441 ggtgcccgct ttggtaacgg ctgtggccca cattttaaaa ccgatataag tggcttccat
     1501 cgggtcatt
//
LOCUS       DAOQGS010000819         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_65041_length_1493_cov_6.595967, whole genome shotgun sequence.
ACCESSION   DAOQGS010000819 DAOQGS010000000
VERSION     DAOQGS010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65041_length_1493_cov_6.595967"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..707
                     /locus_tag="PKZ52_19130"
     CDS             63..707
                     /locus_tag="PKZ52_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HNG61942.1"
                     /translation="MNPSQSGPTPASTQTSTARQTQVSLPTYVTPAPVIRRVMQLPEL
                     PITDLKALWLELFGSPPTTHSRPFLVRRIAYQLQENEFRKVNPELLEQNKKRIEALIE
                     QEKIKNLSSKNDSDYALLPGTVLTREYQRVEHCVVVTEDCQFEYKGRRYKSLSVIARE
                     ITGTRWSGPLFFGLKTLPKSTPKKAKKQKAKLATSTKTKAPTKTRNSQEESRHE"
     gene            700..>1493
                     /locus_tag="PKZ52_19135"
     CDS             700..>1493
                     /locus_tag="PKZ52_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNG61943.1"
                     /translation="MSEMPKRRLRCAVYTRKSSDEGLDQEYNSIDAQRDAGHAYIASQ
                     RSEGWIAVNDDYDDPAFSGGNMERPALKRLMADIEAGKIDVIIIYKIDRLTRSLTDFS
                     AMVGVFERFGVSFVSVTQQFNTTTSMGRLMLNILLSFAQFEREVTGERIRDKIAASKR
                     KGMWMGGVPPLGYDVDSRRLVPNPEEAKLVQHIFKRFVELGSSTLLVKELARDGVTSK
                     SWETQEGRVRQGKPMDKGFIYKLLHNRTYLGELRHKDQWYQTEHTP"
BASE COUNT          419 a          373 c          376 g          325 t
ORIGIN      
        1 ggctttggta gccataagag cgttcatggt caccgcccaa tccctgtaac ttcggagcct
       61 ttatgaatcc atcacaatcg gggccaaccc cggcctcaac tcaaacctca acagcaagac
      121 aaacccaagt atcgcttccc acctatgtca cacccgcacc tgtaatccgc cgcgtcatgc
      181 aactgccgga gctgccgatt acggatctaa aagcgctgtg gctggaatta ttcggctcgc
      241 ccccgacaac acacagtcga ccgtttttag tgcggcgtat cgcgtatcag ttgcaggaaa
      301 atgaatttag aaaagtgaac ccggaattgt tggaacagaa taaaaaacga attgaagcct
      361 taatcgaaca ggaaaaaatt aaaaacctct cctctaaaaa tgacagtgat tatgcattac
      421 ttcctggcac ggtattaacg cgggagtatc agcgcgttga gcactgcgta gtggtaactg
      481 aggactgtca gtttgagtac aagggccgac gctacaaaag cttatccgtt attgcgcgag
      541 aaattactgg tacccggtgg tcagggccgc tattttttgg cttaaaaaca ctgcctaagt
      601 cgacgcctaa aaaagcaaaa aaacaaaaag cgaaattagc cacaagcaca aaaacgaaag
      661 cacccacaaa aacgcgcaat agccaggagg agagccgcca tgagtgagat gcccaagcga
      721 cgcttgcgct gtgccgtcta tacccgcaaa tcctcggatg agggcctaga ccaggaatac
      781 aattccattg atgcccagcg tgatgccgga catgcctata tcgccagcca acgcagtgaa
      841 ggctggattg cagtaaacga tgactatgat gatccggcgt tttccggggg caatatggag
      901 cggcctgcac tgaagcgtct aatggcggat atcgaagcgg gcaaaatcga tgtaatcatt
      961 atttataaaa tagatcgcct gacccgcagc ctgacggatt tttccgccat ggtgggagta
     1021 tttgagcgct ttggcgtgtc gtttgtgtcg gtcacccagc aatttaatac caccacctct
     1081 atggggcggt taatgcttaa catcctactg tcgtttgctc agtttgaacg cgaagtgacg
     1141 ggcgagcgga tccgcgacaa aatcgccgcc agtaaacgca aaggtatgtg gatggggggt
     1201 gtgccgcccc taggctatga cgtggatagc cgccgactgg tccccaaccc tgaagaagct
     1261 aaacttgtgc agcatatatt taagcgcttt gtggagttgg gttcgagcac cttactggtt
     1321 aaagaattag cgcgggatgg cgtgacctcc aaatcttggg agacccagga gggcagggtt
     1381 cgccaaggta aacccatgga taaagggttt atttataaac tgctgcacaa tcgaacttac
     1441 cttggtgagc ttcgccataa agatcaatgg taccagacgg aacacacccc cat
//
LOCUS       DAOQGS010000820         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_65696_length_1485_cov_3.074825, whole genome shotgun sequence.
ACCESSION   DAOQGS010000820 DAOQGS010000000
VERSION     DAOQGS010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65696_length_1485_cov_3.074825"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKZ52_19140"
     CDS             complement(<1..897)
                     /locus_tag="PKZ52_19140"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNG61944.1"
                     /translation="MFIGDQNKGRVIPKSYFGMHVNNALPQNKHPYNSVECPLFADLG
                     YGLIRFWDAYVVHRLVNTANGVYDWVALDYRVNAAISAGLEIIYTFGAPPDWATSTPG
                     PYPNYNPNPVTNINYLSTFATEVANRYRGKIKYYEIYNEVDSAGSWVGSLSVMAQYGE
                     AIYNAVKAQDSAAIVLSPNTISWGSLNALTGMGYLREYLKLASSYCDAIAMHLYTDPS
                     QPESYIGLSKAYLNLAKSFGKQSVMCTETGVLKYFSATGEKKEPRFDGASSIMSESQG
                     ASWVARMLICGWLGGLDGLCYYL"
     gene            complement(908..>1485)
                     /locus_tag="PKZ52_19145"
     CDS             complement(908..>1485)
                     /locus_tag="PKZ52_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61945.1"
                     /translation="ATINCRLTVSCNTVSSAPYVTTITGMANNNNPATAGGPSPYFVT
                     CGGITRPSNQITDTSGVSTPFTFSAGGQLSVKPYSVSESDWNFTVAAGGIVSSASAQP
                     AKAAAGAGIRNFVTWIDVSHDTLSAATELMVLSGSTVIYRLKLQTAASEGKLISLPTP
                     LKGGVNEAINIQLSASVTGGVFVSCGGYSSV"
BASE COUNT          399 a          364 c          331 g          391 t
ORIGIN      
        1 caagtaataa caaaggccgt caagaccccc aagccatccg catatcaaca ttctggcaac
       61 ccatgacgcg ccctgagact cagacataat tgagctggca ccatcaaatc ttggctcctt
      121 tttctcgcct gtcgcagaaa aatacttgag caccccggtc tctgtacaca tgaccgattg
      181 ttttccgaac gattttgcta aattcaaata ggcttttgat aaaccaatgt agctttccgg
      241 ctggctagga tcggtgtata agtgcattgc aatagcgtca cagtagcttg aggcaagctt
      301 taaatactct cgcaaatagc ccataccggt taatgcgttt aatgatcccc acgatattgt
      361 atttggggat aggacaatag ccgcactgtc ctgggcctta actgcgttat agattgcctc
      421 tccatattgg gccatcacac tcaggcttcc cacccatgag ccagccgaat caacctcgtt
      481 gtaaatttcg taatacttaa tttttcccct gtatctattt gccacttctg tagcgaaagt
      541 ggacaggtaa ttaatatttg tgactggatt tggattgtaa tttgggtatg gtccgggtgt
      601 gctagtggcc cagtccggag gcgcaccaaa tgtatagata atttctaacc cggctgaaat
      661 cgcggcatta acacgataat caagagctac ccagtcataa acaccattgg ccgtatttac
      721 aaggcggtgc accacatacg catcccaaaa acgaattaac ccataaccca aatctgcaaa
      781 tagcgggcat tcgaccgagt tgtatggatg cttattttgc ggcagcgcgt tatttacgtg
      841 cattccgaaa taactctttg ggataacgcg ccctttattt tgatcaccaa taaacattaa
      901 gcacccctta tactgaagag tatccgccac agctaacaaa cacgccgccg gtaacggatg
      961 ctgacaattg gatatttatc gcctcattta cgccgccttt tagtggtgtg ggcagggaaa
     1021 ttaatttccc ctcagatgcg gcagtttgaa gttttaatcg ataaataaca gtcgatccac
     1081 ttaaaaccat aagctcagtg gcagccgata gcgtgtcgtg agatacatca atccatgtga
     1141 caaaatttct aattcctgct cctgcagcag ccttagcggg ctgggcagat gcactactaa
     1201 caattccgcc tgccgccacc gtgaagttcc agtcagactc agacactgaa tatggcttta
     1261 cggagagttg accgccggcc gaaaatgtaa acggggtgct tacaccgcta gtatcggtga
     1321 tctggttgga aggtcttgta ataccgccgc aggtgacaaa ataaggcgaa ggcccgccag
     1381 ccgtggcagg attattgttg ttggccatgc cggttattgt ggttacataa ggggctgaac
     1441 ttacggtatt gcatgagact gtaagccgac agtttatagt tgccc
//
LOCUS       DAOQGS010000821         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_66621_length_1473_cov_4.758110, whole genome shotgun sequence.
ACCESSION   DAOQGS010000821 DAOQGS010000000
VERSION     DAOQGS010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66621_length_1473_cov_4.758110"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKZ52_19150"
     CDS             <1..458
                     /locus_tag="PKZ52_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61946.1"
                     /translation="QPDASSSTGVTHRIAFAGTTSRRYAADTYEGLCARYGVWDPSGS
                     WKPAYARNFDGSLNAFATTMTWSPFGEGWTYSGRRHPGSPLPKMAVPMFPSRANGVTA
                     CILGEFNEIMVLTNGYASEETVMPGWIAMIGDDFAQPYAVPAPDAFALV"
     gene            524..>1473
                     /locus_tag="PKZ52_19155"
     CDS             524..>1473
                     /locus_tag="PKZ52_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61947.1"
                     /translation="MTSGPTPLKKRFAAVWGKTEVFVAQTTGWDIATERSWQFRSGWS
                     LQLVHQQRWVYGLRLERRQQIRYGDLHQHRARLSIGYGDQPISRGRLRLVYSDLTLAR
                     QHFHAAYWLTTRLQVAHALPYAIANVNFLRTRFILPWALLDDTHLQAVVNNPELVWQS
                     HTVQLLQATLSCDEDSPVWIASLDIAELADFARIAIGDLITLTLGLEVFKLLIDGKTL
                     SRESSVEPRYTLSAVSPVARLEAPFAHPTPLYQSAAIAAHTAVTNQIGPVDWQLPVWT
                     IPAGRLMLQDVTPLAAARQIVSSIGGLLESQPDGRVVCRRR"
BASE COUNT          274 a          446 c          411 g          342 t
ORIGIN      
        1 tgcaacccga tgccagcagc agtaccggcg tcacccaccg gatcgccttt gccggtacga
       61 cctctcggcg ctatgcggcc gatacttatg aaggcctctg tgcccgttat ggcgtctggg
      121 atcctagcgg aagctggaag cctgcctatg cccgcaattt tgacggctcc ctcaacgcat
      181 ttgccaccac catgacctgg tcaccctttg gggagggctg gacctatagc ggtcgacggc
      241 atccaggatc gccgttgccc aaaatggccg tacccatgtt ccccagtcgg gccaatggcg
      301 ttaccgcctg tatcctcggc gagtttaatg aaattatggt gctgaccaac ggctatgcct
      361 cagaggagac ggtcatgccc ggttggatcg ctatgatagg tgatgacttc gcccagccct
      421 acgccgtgcc cgcaccggat gcctttgctc tggtatgagc accctaagcg cgtcgcttgg
      481 tttaggtgcg tcgctcgcac gtacatggca tctgaatatc catgtgacct cagggccgac
      541 accattaaaa aaacgctttg ccgccgtatg gggtaaaaca gaggtctttg ttgcgcagac
      601 cactgggtgg gatatcgcca ccgaacggtc ctggcagttt cgcagcggct ggtcactcca
      661 gctcgttcat cagcagcggt gggtctacgg gcttaggctt gagcgtcgcc agcagattcg
      721 ctacggcgat cttcaccagc accgtgcacg actgagtatt ggctatggcg atcaaccgat
      781 cagccggggc cgcttgcgat tggtttatag cgatttaacc ctcgcccgcc agcattttca
      841 tgccgcgtat tggctaacca cccggctgca ggttgctcac gctttaccct acgccattgc
      901 caatgttaat tttctgcgca cccgattcat tctgccctgg gcgttgctgg acgatactca
      961 cttgcaagcg gtggtgaata atcctgagct ggtatggcag tcgcacacag ttcaattgct
     1021 gcaagccacc ctttcctgcg atgaggacag cccggtgtgg atcgcttcct tagatattgc
     1081 cgagttagcg gattttgcgc gcatcgccat cggggatctg atcacgctca ccttggggtt
     1141 ggaggtattt aagctactga tcgatggcaa aacgctctcc cgtgaatctt cagttgaacc
     1201 gcgttacacc ctgtcggcgg tgtcgcccgt ggctcgttta gaagcaccct ttgcccatcc
     1261 gacaccgctt tatcagtcgg cggccatcgc cgcccacacg gcagtgacaa atcaaatagg
     1321 gccggtcgat tggcaattac ccgtctggac gattcccgcc gggcgcttga tgctgcagga
     1381 cgtcacgcca ctggccgccg cgcggcagat tgtcagttcc atcggtggtc tgctggagag
     1441 ccaacccgat ggccgtgttg tctgtcgacg ccg
//
LOCUS       DAOQGS010000822         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_67279_length_1465_cov_4.719858, whole genome shotgun sequence.
ACCESSION   DAOQGS010000822 DAOQGS010000000
VERSION     DAOQGS010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67279_length_1465_cov_4.719858"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PKZ52_19160"
     CDS             <1..1163
                     /locus_tag="PKZ52_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61948.1"
                     /translation="EPFELLYPVTPILNQAIGFEVVFELHNLTTFRWVVQLTNHLDFT
                     VGLIVQAAGWQAWITLAAGQTRYLSGATAVSGMGNWNAYPLHPFSPLFGWHRNWGNEF
                     IRMLDGECQVSFRVYLTAPDQADCIPPGLPALQAGLRWRANPTDLELSFTPRWSAIKR
                     FSRSGVYEYDFDNADDPILERTPAIRLPAYAMLDPATYFAQGYDTAPGDTSPWHKRPW
                     SQALQMPLMCQGEKLSAEGLETLWVAADDIALLIQHEHLTWVAAHLGIAEAVIMDGND
                     ISWFDIGPNNTWHNLLWINRFYTLAGVAVDEAAMRAAFNAMLAHFSDDWPNYYRTLRV
                     HQAVRFGTFLDNGAAEMGTYAINETGNLEEANTLDVNAVSTRFLYRINTFTG"
     gene            1281..>1465
                     /locus_tag="PKZ52_19165"
     CDS             1281..>1465
                     /locus_tag="PKZ52_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61949.1"
                     /translation="MRVVLFYFAAYFFSTIALSTTTMIQLDGLVLPSGLRWSDEFTTT
                     SVAQSARRTLDGSLVVF"
BASE COUNT          300 a          395 c          383 g          387 t
ORIGIN      
        1 tggagccgtt tgagctgctc tacccggtca cacccatcct caaccaggcg attgggtttg
       61 aggtggtgtt tgagctacac aatctcacca cctttcgctg ggtagtccaa ctgaccaatc
      121 acctggattt tacggtgggg ttaatcgtgc aagcggcagg ctggcaggcc tggataaccc
      181 tggccgctgg gcaaacccgc tacttaagcg gcgccacagc ggtgtccggt atgggtaact
      241 ggaatgccta tccgctgcac ccgttttcac cactcttcgg ctggcaccga aactggggca
      301 atgagtttat ccgcatgctc gatggggaat gccaggtgtc attccgggtg tacctcacgg
      361 caccggatca ggccgactgc attccacctg gattgcctgc cttacaggcc gggctgcgct
      421 ggcgcgccaa ccccacggat ctggaactga gttttacccc gcgctggtct gcgataaagc
      481 gcttctcacg cagcggcgtg tatgaatacg attttgataa tgccgatgac ccgattctgg
      541 agcgaacgcc cgccatccgc ctgcccgcct acgccatgct cgatcccgct acttattttg
      601 cccagggcta cgataccgca ccgggtgata ccagtccctg gcataagcgg ccctggtccc
      661 aggctctgca gatgcccctc atgtgccagg gagagaaact cagtgccgag ggattggaaa
      721 ccctttgggt ggcggctgac gacattgccc tgctcatcca acacgagcat ctgacctggg
      781 tggcggcgca cttggggatt gctgaggcgg tgatcatgga tggcaacgat atcagctggt
      841 ttgacatcgg accgaataac acctggcaca acctgttgtg gattaaccgt ttttataccc
      901 tcgctggagt tgccgtcgat gaagcggcga tgcgcgcggc gtttaacgcc atgctcgccc
      961 acttcagcga cgactggccc aactattacc gcacgttacg tgttcatcaa gctgtgcgtt
     1021 ttggcacttt tttagataat ggcgcggcag agatgggcac ctatgcaata aatgaaacgg
     1081 ggaatttgga ggaggcgaat accttggatg tcaatgcggt gagcacacgg tttttgtacc
     1141 gcattaacac tttcacaggc taaaaacgat ttttattttt tattttttat tttttggtcg
     1201 cccacaactg taagcagcgc tttaaatacc gcattcaatt tattgtttaa ttacgttttt
     1261 aatatcattt taaatggtca atgcgtgtcg ttttatttta tttcgccgcc tatttttttt
     1321 ctactattgc cttatcgacc accaccatga ttcagctaga cggccttgtt ttacccagcg
     1381 gtttacgctg gagtgatgag tttactacta cgtcggtggc ccaatccgcg cgccgtactt
     1441 tagatggatc cctggtggtg tttta
//
LOCUS       DAOQGS010000823         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_67633_length_1461_cov_8.266714, whole genome shotgun sequence.
ACCESSION   DAOQGS010000823 DAOQGS010000000
VERSION     DAOQGS010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67633_length_1461_cov_8.266714"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..780)
                     /locus_tag="PKZ52_19170"
     CDS             complement(163..780)
                     /locus_tag="PKZ52_19170"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNG61950.1"
                     /translation="MHNATAKTITLAITGASGADYGLRLLQQLIAAKCRVYLLISQAA
                     QVVIRTETTLLPPLELTQQHAYWEHWSGAGPGQLSLLARDDWFAPVASGSSSSASMVI
                     CPASGGCISAIACGASNNLIERAADVALKERRQLIIVPRETPYSQIHLENMLKLTQMG
                     AIILPASPGFYHKPKAIGDLVDFIVARILAQLGIEQTLLAPWGEY"
     gene            complement(773..>1461)
                     /locus_tag="PKZ52_19175"
     CDS             complement(773..>1461)
                     /locus_tag="PKZ52_19175"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNG61951.1"
                     /translation="KALQQVLAQGLWTPTQTLGIGGDWQAKLINADGSCFEVYFQGEK
                     VGSVNWSLLGNHNIANALAAMAAARHVGVEPHTGCAALGEFLGVKRRLELVGEAKGRK
                     IYDDFAHHPTAIATTLKGLRAKVGNERIIAIIEPRSNTMKQGVHAQTLGLSCQAADEV
                     IWFEPPGLHWSLREVAKNAGNKQKAFCSVEEIIEYTLRESRCGDHILIMSNGGFAGLH
                     GTLLEALRDA"
BASE COUNT          354 a          405 c          332 g          370 t
ORIGIN      
        1 gccctagcgt cgttgaattt ggcaaaaaaa atgctcattt actgcgtgta aactcggcaa
       61 ttgctcctgc attgctctac ctcctgcctc cctgcagtcg tccgcttttt ttgccaaatt
      121 cgcctagcta gtgcttcgcc gcctacattt ttcagaggtt ccctagtatt cgccccaggg
      181 cgcaagtaaa gtttgttcga ttcccaactg agccagaatg cgcgccacaa taaaatccac
      241 caaatcacca atggcttttg gcttgtgata aaacccaggg ctagcaggta aaataatagc
      301 gcccatctgg gtgagtttaa gcatgttttc caggtgaatt tgtgaatagg gcgtctcccg
      361 cggcacaata attaattgcc gccgttcttt taacgccaca tccgccgcgc gctcaattaa
      421 attattgctg gcgccacagg caatggcgga aatgcagccg ccgctggccg ggcaaatcac
      481 catactggca gagcttgacg agcctgatgc cacaggtgca aaccaatcat cccgcgccaa
      541 taaacttaat tgccctgggc ctgcgccgct ccagtgttcc caataggcat gctgctgggt
      601 taattccagg ggtggcaaaa gtgtggtttc cgtgcgaatc accacctgtg cagcttgaga
      661 aatcaataaa tacacgcggc atttagcggc aattaattgc tgtaataaac gcaggccgta
      721 atcagcaccg gaagcgccgg taatggcaag ggtaatggtt tttgcggtgg cgttatgcat
      781 cgcgcagagc ctctaataac gtgccatgca atccggcaaa accgccgtta ctcataataa
      841 gaatatgatc gccgcagcgg ctttcgcgca gggtatattc aataatttcc tccaccgaac
      901 aaaatgcctt ttgtttatta ccggcatttt tagccacttc ccgtaaggac caatgtaaac
      961 ccggcggttc aaaccaaatc acttcatcgg ccgcttggca acttaacccc agcgtctgcg
     1021 cgtgcacacc ttgtttcatg gtattcgagc gcggttcaat aatggcgata attcgttcat
     1081 tccccacttt tgcccgcagg ccttttaagg tggtggcgat ggcggttggg tgatgggcaa
     1141 aatcatcgta aatttttcgg cccttagctt cgcctaccaa ttctaaacgg cgttttaccc
     1201 ctaaaaattc cccaagtgcg gcgcaaccgg tgtggggctc cacacccaca tggcgcgccg
     1261 ccgccatagc agccagtgca tttgcgatat tatgattgcc caacaatgac cagtttacgc
     1321 taccgacttt ttcaccttga aaatacacct caaaacaact gccatcagca ttaattaatt
     1381 tagcctgcca atcgccaccg attcccagag tttgggttgg cgtccacagg ccttgcgcca
     1441 atacctgttg cagggcttta t
//
LOCUS       DAOQGS010000824         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_67841_length_1459_cov_3.157407, whole genome shotgun sequence.
ACCESSION   DAOQGS010000824 DAOQGS010000000
VERSION     DAOQGS010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67841_length_1459_cov_3.157407"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PKZ52_19180"
     CDS             <1..164
                     /locus_tag="PKZ52_19180"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNG61952.1"
                     /translation="TTGRHDPCVGIRATPIAEAMMAIILLDHALRHRGQNSDVALSVP
                     IIPAQAPEA"
     gene            262..1437
                     /locus_tag="PKZ52_19185"
     CDS             262..1437
                     /locus_tag="PKZ52_19185"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNG61953.1"
                     /translation="MQHYWRLACVYAAYFAVLGAMVPYWSLYLHAREFNPAEIGILLA
                     IPTLTKIMVPNLWGYIADRSGHYKGIIVLGALGAWLAFSGIYWAQNFAAIILVMTLYS
                     AFWNAILPQFEALTLHSLAHQPESYTRIRLWGSLGFIALVLGLGWLFDRLSVLNLPII
                     LTALITLLCVVCVGLPPVELHRSTVVPQTLKSVMADAQVRRFFVSTLLYQASHGIYYG
                     FYSIYLHQHGYSASVIGALWTLGVAAEIVLFWWIPKVLPSLNLPRTAQLCMLVAAVRW
                     TLIGFTVDYWPCLILLQLLHAVTFGLFHASSVQFIRQSFGPALQGQGQALYGAIGYGA
                     GGALGLWLCGLLWQIGSQWAFVFAVSCAFLAAGVQRGGVWQVLKKPPLLTRRQNRSS"
BASE COUNT          266 a          333 c          413 g          447 t
ORIGIN      
        1 ttactaccgg ccgccacgat ccctgtgtgg gtattcgtgc cacacccatt gctgaagcca
       61 tgatggcgat tattttattg gatcacgccc tgcgtcatcg cggccaaaac tcggatgtgg
      121 ccttgagtgt gcccattatt ccggcccaag cgccagaggc gtaaagccaa tttttaagcc
      181 cccgccttaa cctgtgcacc gtttgtagct ggagcggtgc cgcgtatttc ttagcgactg
      241 aacgatagag tttaaattct tatgcagcat tattggcgct tggcctgtgt gtatgccgcg
      301 tattttgctg tgctgggggc catggtgccc tactggagtt tgtatctgca cgcgagggaa
      361 tttaatcctg ccgaaatcgg tattttatta gctattccca cgttaaccaa aataatggtg
      421 cccaacctct ggggttatat tgccgatcgc agtgggcact ataaaggcat tattgtgctc
      481 ggcgccttgg gtgcttggct ggcctttagc ggtatttatt gggcgcagaa tttcgctgca
      541 attattctgg tcatgaccct ctacagcgcg ttttggaacg ccattttgcc gcagtttgaa
      601 gcgctcacct tgcacagtct ggctcatcaa cccgagagtt acacgcgtat tcgcctgtgg
      661 ggttccctcg gttttatcgc tttggtattg gggttggggt ggttgttcga tcgcttatcg
      721 gtactgaatc tgccgattat tttgacggca ttaatcactt tgttgtgtgt ggtgtgtgtg
      781 ggtttgccgc cagtagaatt acatcgctca actgtagtac ctcaaacgct taaatcggtt
      841 atggcagatg ctcaggtgcg ccgatttttt gtaagcacac ttttgtatca ggccagccac
      901 ggcatttatt acggctttta cagtatttat ttacatcaac acggctatag cgccagtgta
      961 atcggcgccc tgtggacatt aggggtggcg gcggagattg ttttattctg gtggattcct
     1021 aaagtattgc ccagcttaaa ccttccgcgc actgcgcagc tgtgtatgtt agtggcggcg
     1081 gtgcgttgga cattaattgg ttttactgtg gattattggc cgtgtttaat tctgttacag
     1141 ctattgcacg cggttacgtt tggtttattc cacgccagca gtgtgcagtt tatccgccag
     1201 agtttcggcc ctgcgctgca aggccagggg caggcattgt acggcgcaat tggttatggc
     1261 gcaggcgggg ctttggggct ttggttgtgc ggcctgttgt ggcaaattgg ctcccagtgg
     1321 gcgtttgttt ttgcggtgag ttgtgcgttt ttggcggcgg gggtgcagcg gggcggtgtt
     1381 tggcaagtat taaaaaagcc gccgttatta acccggcggc aaaatagatc gtcctgatct
     1441 attttgccta ggctgcgct
//
LOCUS       DAOQGS010000825         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_68208_length_1454_cov_3.849178, whole genome shotgun sequence.
ACCESSION   DAOQGS010000825 DAOQGS010000000
VERSION     DAOQGS010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68208_length_1454_cov_3.849178"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PKZ52_19190"
     CDS             complement(<1..1233)
                     /locus_tag="PKZ52_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNG61954.1"
                     /translation="MAIETIKVPDIGGAEGVEVIEVCVKPGDSVSAEQSLIVLESDKA
                     SMEVPSPKAGKVVSVLIKVGDKASEGTAVIELEVAGAAAAPAPAAAAAPAAAPAASAP
                     AASAELAVAVPDIGGAEGVDVIEVCVKVGDQVKEGDSLIVLESDKASMEVPSPASGTV
                     LSLAIKVGDKASQGTPVLVLASGAAPVAPAPTAAPAPVAAAAGPVAVAVPDIGGAEGV
                     EVIEVCVKAGDEVKEGDSLIVLESDKASMEVPSPASGKVISLAIKVGDKASQGTPILQ
                     LAVAGAAPAAAPTPAASAPASKPAPVASSLAPQASAPVAAETRGASSDYYAGPSVRML
                     ARQLGVNLAEVKASGPRGRIVKDDVKAFVKAVMENRGKAPAGGSAVSGVGIPQVPEID
                     FSQFGPIELVKMSKIKKLT"
     gene            complement(1249..>1454)
                     /locus_tag="PKZ52_19195"
     CDS             complement(1249..>1454)
                     /locus_tag="PKZ52_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61955.1"
                     /translation="YYVLGTDGFGRSDTRTKLRRFFEVDRYYVTVTALKALADQGKVD
                     VKEVVDAMKKYGLDAEKVDPMIC"
BASE COUNT          277 a          457 c          398 g          322 t
ORIGIN      
        1 ggtgagcttt ttaattttgc tcattttcac cagttcaata gggccaaact gggagaagtc
       61 gatttccggc acctggggaa tccccacacc gctcactgca gaaccacctg ctggcgcctt
      121 gccgcgattt tccatcacgg ctttaacgaa ggcttttacg tcatctttaa cgatgcgccc
      181 gcgcgggccg gaggctttta cttcggccag gttcacaccc agctgacggg ccaacatacg
      241 cacggagggg cctgcgtagt aatcgctgct ggcgccgcgg gtttcagctg cgacaggcgc
      301 gctggcttga ggtgcaaggc tgctggcaac tggcgcaggc ttactggcag gcgcggatgc
      361 ggcaggcgtt ggcgccgctg cgggtgctgc accggccacg gccaactgca gtatgggtgt
      421 accttgcgag gccttatcgc ccaccttgat ggctaggctg atcaccttac cggaggctgg
      481 ggacggcact tccatggagg ccttatcgct ctccaacaca atcagggagt cgccttcttt
      541 gacctcatcg cccgctttta cacagacttc gattacctca accccttcag cgccgccgat
      601 atccggcacc gccaccgcta ctgggcctgc ggccgcagca accggtgcgg gtgcagcggt
      661 gggcgctggc gcaaccggcg ccgcaccgct ggcaagtacc aataccgggg tgccctggga
      721 ggccttatcg cctactttaa tagccaggct caacacggtt ccgctagcgg gcgatggcac
      781 ttccatagaa gctttatcgc tttcaagcac aatcagtgaa tcgccttctt taacctggtc
      841 gcccacctta acgcagactt caattacatc tacgccttcg gcgccgccta tatccggcac
      901 ggccacggcc agttccgcac tagccgcagg tgcgctagca gcaggagccg ccgcaggtgc
      961 agcagctgca gcgggagctg gcgctgccgc cgctccggcc acttccagct cgataacggc
     1021 ggtgccttcc gaggctttat cgcccacttt gatcaataca ctcaccacct tgcccgcttt
     1081 gggggaaggc acttccatgg aggctttgtc gctctccaac acaatgaggg attgctcggc
     1141 gctaacgcta tcaccgggtt taacacagac ttcaattact tctacacctt cggcgccgcc
     1201 aatatcgggc accttaatgg tttcaatagc cacgatttaa gcctccgatt agcagatcat
     1261 aggatcgact ttttcagcgt cgagtccgta ttttttcatt gcatcaacca cttcttttac
     1321 atccaccttg ccttgatcgg cgagggcttt taaagcggtg acggtgacgt agtagcgatc
     1381 cacttcgaaa aaccggcgca gtttggtgcg ggtgtcgctg cggccgaagc cgtcggtgcc
     1441 cagcacgtaa taat
//
LOCUS       DAOQGS010000826         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_68209_length_1454_cov_3.834882, whole genome shotgun sequence.
ACCESSION   DAOQGS010000826 DAOQGS010000000
VERSION     DAOQGS010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68209_length_1454_cov_3.834882"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKZ52_19200"
     CDS             <1..282
                     /locus_tag="PKZ52_19200"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG61956.1"
                     /translation="AQVYLRQLNKIYPSLQQFYNERHGMFYWPEHPHTLGSYACYKVG
                     QWSAFAGVEAEPIGHVYFAGEHCSAGFQGYMEGAAETGLNAALAVLKAL"
     gene            636..>1454
                     /locus_tag="PKZ52_19205"
     CDS             636..>1454
                     /locus_tag="PKZ52_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNG61957.1"
                     /translation="MEFLDLPAASLAAGTVTLPGSKSISNRTLLLAALSQGETLIHDL
                     LASDDTDRMLEALATLGVKVEQLGANQYRVTGCGGQFPVKEADLFLGNAGTAFRPLTA
                     ALAFSGGTYTLRGVPRMHERPIGDLVDALRQLGADITYLDQAGFPPLLIKPAAPQAVS
                     SVSIRGDVSSQFLTALLMALPTLGQAAHITMASELISKPYIEITLNLMAQFGVAVARS
                     GWEAFHIAEGQTYTSPGQVFVEGDASSASYFLAAGALGGGPVRVMGVGKNSIQGD"
BASE COUNT          279 a          453 c          400 g          322 t
ORIGIN      
        1 gcccaggtgt atttgcgcca actcaataaa atttacccca gcctgcagca gttttataac
       61 gagcgccacg gcatgttcta ctggccggag cacccccata ccctcggcag ctacgcctgt
      121 tataaggtgg ggcagtggtc ggcatttgcg ggagtcgagg cagaacccat cggccatgtg
      181 tactttgccg gggagcattg ctcagcgggc tttcagggct atatggaagg cgctgccgag
      241 acgggtttaa atgcggcttt ggcggtatta aaagcgcttt gatcagcttc gcagtattac
      301 cctatccaga ctatgtcatt aggttaaggc cagctgtagg ggcagccctt gcggttgccc
      361 agtttttacc tcggctactc attaggattg gcgcgggggc gggcacccac aaggggtgcc
      421 tctacggctt tctgccatgc aggcaatcct taacttaatg acactgccta tccagaagtg
      481 aatgggttaa ggcttggctc acatctaggc gccatgggcc aaacacccat ctgagcccgc
      541 caggcacttc ctcagccctc tgctgcattg tgtttcctaa gtgagttcgc tagccttgcc
      601 agccttaatt aattcccccc ttcacacggt accctatgga attcctcgac ctgcccgccg
      661 ctagcctcgc cgctggcacc gttaccctgc ccggctctaa aagtatctcc aaccgcaccc
      721 tgctactggc ggccttaagc caaggcgaaa cactcattca cgacctgctc gcctccgacg
      781 ataccgaccg catgctcgag gccctggcta ccctcggcgt caaggtggag cagctcggcg
      841 ctaaccaata ccgggtaacc ggctgcggcg ggcagtttcc ggttaaggaa gcggatttat
      901 ttctcggcaa tgccggtacc gcctttcgcc ccttaaccgc cgccctggcc tttagcggcg
      961 gcacctatac cctgcgggga gtgccacgca tgcacgagcg ccccataggc gatctagtgg
     1021 atgccctgcg gcagttgggt gcggatatca cctatcttga ccaagcggga ttccctcccc
     1081 tattgattaa gcccgccgcc ccccaggccg tctccagcgt atccattcgc ggcgatgtct
     1141 ccagccaatt tctcactgcg cttttgatgg cgctgccaac cttgggccaa gcggcccata
     1201 tcaccatggc cagcgaactc atctccaaac cctacattga aatcaccctc aacctgatgg
     1261 cccagtttgg agtagcggtg gcgcgcagcg gttgggaggc gttccatatt gccgaaggac
     1321 agacctacac cagccccggc caggtatttg tggaggggga tgcctcctcg gcgtcttatt
     1381 tcctggcggc gggcgcctta ggcggcggcc cggtgcgggt gatgggtgtg ggtaaaaaca
     1441 gtattcaagg ggat
//
LOCUS       DAOQGS010000827         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_68702_length_1448_cov_4.822685, whole genome shotgun sequence.
ACCESSION   DAOQGS010000827 DAOQGS010000000
VERSION     DAOQGS010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68702_length_1448_cov_4.822685"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..829
                     /locus_tag="PKZ52_19210"
     CDS             80..829
                     /locus_tag="PKZ52_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG61958.1"
                     /translation="MLAFLFASLGMGLFGSLHCLGMCGGLMAALSFGIPAQSSRLPLM
                     LLASAGRVISYGLMGALAGALLQSFAIGPWPRLVAAVLLILTGLYLAGWWNLLTRLET
                     LGAALWRRLEPLRKRCLPIDSSPKAIGFGLLWGALPCGLVYSALTYTATAAPSNAPMP
                     QALAAALGMLAFGLGTSPAVLIGGAMASQLKSWLQKPLLKPLLGVSYIAFGLWTGFTA
                     FGHHHHHPMEPSSTHGQHEGHDGHMNHEHHH"
BASE COUNT          308 a          409 c          376 g          355 t
ORIGIN      
        1 cgcagcagaa cccagggcca gagcctgtag cttctacacc tgaaccgcca aaacctgagc
       61 cagttaagga gccctagaga tgttggcgtt tttatttgcg agcctcggca tgggactgtt
      121 tggctccttg cattgcttgg gcatgtgcgg cggccttatg gcggcgttaa gctttggtat
      181 ccccgcccag tcctcccgtt tgcctttgat gttactggcc agtgcagggc gggttatctc
      241 ctacgggctg atgggcgcac tggcaggggc attattacaa agctttgcca taggcccttg
      301 gccgcgcctt gtggcggctg tgctgttgat actaacgggg ctgtacctcg ccggttggtg
      361 gaacctgctc acccgtttag aaaccctcgg cgcagccctc tggcgcaggc ttgaacccct
      421 gcgcaaacgc tgcctgccca tcgattcaag ccccaaggct atcggctttg gcctgttatg
      481 gggggcgctg ccctgcggcc tggtgtattc agccctcacc tacaccgcca ctgctgcacc
      541 ctccaatgca cccatgcccc aggcgctagc ggccgcttta ggtatgttgg cctttggcct
      601 gggaaccagc cccgctgtat taatcggcgg cgccatggcc agccaactca aatcctggct
      661 gcaaaaaccc ctgttaaaac ccctgctggg ggtgagctac atcgcctttg gcctatggac
      721 tggctttaca gcctttggcc atcaccacca tcaccccatg gagccctcat ccacccacgg
      781 ccagcatgaa ggccatgacg ggcatatgaa ccacgagcac caccactaaa ggccaatacc
      841 taataatgcg cgctagggtt gagggagtta aatgcattgg tttggtcggc gctattttta
      901 tccaaaacca caagctccca ttttaaaaaa tccctgcggt atggccttag ccatgttgct
      961 atttcttgcc ggtttttggc cgaaccctca gctgcggcaa attaccactg ggcttaaata
     1021 attgctgctt gtgatattgg gcgcgttatt aaagcgttga gtaactattc attactgtgg
     1081 gggctccagc acgacagcaa tcgcctgtct gagcatgcat cacgtccagt taaactagaa
     1141 aggtacggaa attaattatt gttatccggt gttattaaat tgcggtagcc cgtcgccaca
     1201 agccctatta aagtgaaaaa tccacgcatg tttgccaact tattttgcgg tggcagcgaa
     1261 gcagaaaatc tccaggttat taactcggcg gcattaagta ccgggttgat aatggcggca
     1321 tggatgccgc gccaaaggtg cgaaccgctg cgtgagcata atagcgatgg cctggcggta
     1381 aaaattattg cacttattaa cccggcggct aaatagatcg tcctgatcta tttagccttc
     1441 gctgccct
//
LOCUS       DAOQGS010000828         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_69867_length_1435_cov_2.823188, whole genome shotgun sequence.
ACCESSION   DAOQGS010000828 DAOQGS010000000
VERSION     DAOQGS010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69867_length_1435_cov_2.823188"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKZ52_19215"
     CDS             complement(<1..1060)
                     /locus_tag="PKZ52_19215"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNG61959.1"
                     /translation="MRTLIWLLAAAFSLGSACSWADSLTARVDRTNLSLEQTLNLQLT
                     YDGRSNDEPDLTALEQNFEILSRSHSSSFTMSNGQASSSTRWNLTLAPKMAGQLLIPP
                     LNLEGAQSKPIAITVSEASEPVHATDKDLFIETSVDKSSAYVQEQVLVSYKFYYDLPV
                     SRLEPAAIQVADIQVRPLPQAEYTANVGHRTYQVAEFKYVVTADKSGDITLPATTWTA
                     YAQDPSASMFGMRGGKQDIHRLKTDELHLKIKPKPASYPAGVPWLPAKALNLDQEWSR
                     KTDQLTQGEPVTRTLTITAEGLAAEQLPPIGENLSISGLKIYPDKPQLDTQTGPNGLS
                     STRIDSLSVVPNQSGTLEV"
     gene            complement(1077..>1435)
                     /locus_tag="PKZ52_19220"
     CDS             complement(1077..>1435)
                     /locus_tag="PKZ52_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61960.1"
                     /translation="DQQEDQQQADKSAGQQGDKDKQAAEAKAAAQQKAQEEYAKKLAE
                     AAQDKQDEATPAEAGTAAESKEQQENQQKIDQALRRVPDDPGGLLRNKFQYQYKERRM
                     NPQAPKDADNNPENRI"
BASE COUNT          237 a          349 c          461 g          388 t
ORIGIN      
        1 gcacttctaa ggtgccgctt tgattgggta ccaccgagag gctgtcgata cgggtagagg
       61 agaggccatt ggggccggtt tgagtgtcca gctggggttt atccgggtag atttttaacc
      121 cgctgatact gaggttttct ccaatggggg gtaattgctc tgcggctagg ccttcagctg
      181 tgatggtcag ggtgcgcgtt accggctcgc cctgggtgag ctggtcggtt ttgcggctcc
      241 attcttggtc gagattcaaa gctttggccg gcagccaggg cacaccggct gggtagctag
      301 ccggtttggg tttgattttt aggtgcagct catcggtttt taagcggtga atatcctgct
      361 tgccgccgcg catgccaaac atgctggcgc tggggtcttg ggcgtaggcg gtccaggtgg
      421 tggcgggcag ggtgatgtca ccgcttttat cggcggtgac cacatattta aattccgcca
      481 cttgataggt gcggtggccc acattggcgg tgtattccgc ctgaggtaag ggccgcactt
      541 gaatatcggc cacttgaatg gccgcaggtt ctaaacggct taccggcaga tcgtagtaga
      601 acttgtaact caccagcacc tgctcttgta cataggcgga ggacttatcg acgctggttt
      661 cgataaacag gtctttatcg gtggcgtgca ccggctcgct ggcttcgctg acggttatgg
      721 cgatgggttt gctttgggcg ccctctaggt tcaggggggg gatcagcagt tggcccgcca
      781 ttttgggcgc gagtgtaagg ttccagcggg tgctggagga ggcctggcca ttgctcatgg
      841 taaaggacga agaatgggag cgggagagaa tctcgaaatt ctgctccaag gcggtaagat
      901 ccggctcatc gttgctgcgc ccatcgtagg tgagctgcaa attcaaggtt tgctcaaggc
      961 tgagattagt gcgatccacc cgggccgtga ggctatcggc ccagctgcag gcactgccaa
     1021 ggctgaaagc cgcagcaagc aaccaaatta atgtgcgcat aaaattcagc ataggcttag
     1081 atacggttct cagggttatt gtcggcatct ttgggcgctt gggggttcat gcggcgctct
     1141 ttgtattggt attggaattt attgcgcagc agcccgcctg gatcgtcggg cactcggcgc
     1201 agggcctggt caattttctg ttgattctcc tgctgctctt ttgattcggc agcggtgcca
     1261 gcctcggcag gcgtggcctc atcttgcttg tcttgggccg cttcggcgag ctttttagca
     1321 tattcctctt gagccttttg ttgggcagcg gcctttgctt cggcggcttg tttatctttg
     1381 tcgccctgtt gcccggcgga tttatcggcc tgttgttgat cctcctgctg gtcct
//
LOCUS       DAOQGS010000829         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_70022_length_1433_cov_4.656749, whole genome shotgun sequence.
ACCESSION   DAOQGS010000829 DAOQGS010000000
VERSION     DAOQGS010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70022_length_1433_cov_4.656749"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..>1433)
                     /locus_tag="PKZ52_19225"
     CDS             complement(77..>1433)
                     /locus_tag="PKZ52_19225"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNG61961.1"
                     /translation="ILAELAQVEMHAVQTSGNCIRNVTSDQFAGIAPDEIEDPRPYCE
                     IIRQWSTFHPEFAFLPRKFKIAVTGAAEDRAAIAFHDIGLRLTKNSDGDIGFQVFVGG
                     GLGRTPVIGSLIREFLPENDLLTYLESIVRIYNLHGRRDNKYKARIKILVRAMGVETF
                     TKMVEDEWAHIRDSQTLLTDKEIKRAKSFFTEPDYQAFDAGAVWAQVNGLRSDDKSFA
                     HWLDHNTHAHKIPGYRAVTLSLKPTGVAPGDITDGQLEAIADLAERYSFGEVRVTHYQ
                     NIVLADVQVQELHALWLELKTLGFATANIGTLTDIICCPGGDYCSLANAKSIPIAEAI
                     QREFEDLDYVYDLGDIDLNISGCMNACGHHHIGHIGILGVDKKGEEFYQIQLGGNSKT
                     QAALGEVLGPSFGAAEVPAIIRKILEVYLAARSDEESFLDTYQRIGLSPFKEQIYAKT
                     H"
BASE COUNT          345 a          346 c          381 g          361 t
ORIGIN      
        1 tagtgtgtaa tcattaatat cgccagtagc gccctgctct gctatcaccc aggggttagc
       61 cgtaaattcg ccatttttaa tgagttttgg catatatctg ctccttaaaa ggtgaaagac
      121 cgatgcgctg gtaggtatcc aaaaaacttt cctcatcgct gcgggcggcg aggtaaacct
      181 ccagaatttt acggataatg gcgggaacct cggcggcacc aaatgagggg cctaaaactt
      241 cgccgagggc cgcttgggtt tttgaattgc cgcccaattg aatctggtaa aactcttcgc
      301 cttttttatc tacgcccaga ataccgatat ggccgatatg atggtggccg caggcattca
      361 tacagccgga gatattaagg tctatgtcgc ctaagtcgta cacataatcc aagtcctcaa
      421 attcgcgctg aatagcttcg gcgataggga tggatttagc gttggctaag gagcagtagt
      481 ctccgccggg gcagcaaata atatcggtga gtgtgccgat attagcggtg gcaaagccga
      541 gggtttttaa ttccagccac agagcgtgga gttcttgcac ctgcacatcg gcgagcacaa
      601 tattttgata gtgggttacg cgcacttcgc caaatgaata gcgctccgct aaatcagcga
      661 tggcctctaa ctgaccgtcg gtgatatcgc caggagctac acctgttggt tttaaggata
      721 gggttacagc gcgataaccg gggattttat gggcgtgggt gttgtgatcc aaccaatggg
      781 caaaagactt atcgtcgcta cgtaggccgt tgacttgcgc ccaaacagcg ccagcgtcaa
      841 atgcctgata atccggctcg gtaaaaaaac ttttcgcgcg cttaatttct ttatccgtca
      901 gcagggtttg gctatcgcgg atatgcgccc attcgtcctc caccattttg gtaaaggttt
      961 ctacacccat ggcgcgcacg agaattttaa tgcgcgcttt gtatttgtta tcgcgacggc
     1021 catgcaggtt ataaatgcgg acgattgact ctaaataggt gagcagatca ttctcaggta
     1081 aaaattcccg aatcaagctg ccgattactg gggtgcgacc caagccgccg cccacaaaca
     1141 cctgaaaacc tatatcacca tcgctatttt tggttagtcg caggcctata tcgtggaacg
     1201 caattgcagc gcggtcttcg gcggcgccag tgacggcaat tttgaatttg cgcggaagga
     1261 aagcgaattc cgggtgaaag gtagaccact ggcgaatgat ttcgcagtag ggacgcgggt
     1321 cttcgatttc atccggggcg atgccggcaa actgatccga ggtgacattg cggatacagt
     1381 tgccgctggt ttgtaccgca tgcatttcca cttgggccaa ctcggccagg atg
//
LOCUS       DAOQGS010000830         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_70307_length_1430_cov_3.157091, whole genome shotgun sequence.
ACCESSION   DAOQGS010000830 DAOQGS010000000
VERSION     DAOQGS010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70307_length_1430_cov_3.157091"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKZ52_19230"
     CDS             <1..287
                     /locus_tag="PKZ52_19230"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG61962.1"
                     /translation="TGPSADAGYLNNPAPTYPELAQERGWEGSVLLSVVVLPNGKPKS
                     VEVKQSSGRKVLDDAAVATVKNWSFVPAKLGNETIEASVEVPIDFRLANN"
     gene            317..1051
                     /locus_tag="PKZ52_19235"
     CDS             317..1051
                     /locus_tag="PKZ52_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNG61963.1"
                     /translation="MSELYKHIVEWTLWALIAFSLATWTLILAKSVQHFLIGRQNRIF
                     TKAFWSAADLNAAAALEKYVGPTARLAAIGFSTLHNADAHTASNLETSWDRQDLLERH
                     LRQQIQKERRSLESGLAVLASVGSTAPFVGLFGTVFGIIMALTAISSKSSASIEVVAG
                     PIGEALVATGVGIAVAVPAVLSYNFFLRRLKLIAADLDDFATDFITLVQKSGFRIQVP
                     TTNKSTHSNRAEKPAAKLDVVQEVLA"
     gene            1051..>1430
                     /locus_tag="PKZ52_19240"
     CDS             1051..>1430
                     /locus_tag="PKZ52_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNG61964.1"
                     /translation="MAISTSNGDSEEVFGEINVTPLVDVMLVLLVTFIITAPMLTNTI
                     NVNLPETQPTLSQKEPEKPTVISVDEAGQVYVDKQPFAVEALEADLTAKKTINPEIRL
                     NLNADEKVPYGVIAKVMAAIERSG"
BASE COUNT          386 a          347 c          330 g          367 t
ORIGIN      
        1 ccactggccc ctcggcggat gccggttact taaataatcc cgcccctact tatcctgagc
       61 ttgcccaaga gcgcggttgg gaaggctccg tgttattaag tgtagtagtg ctacccaatg
      121 gcaaacctaa atcggtagaa gttaaacaat ccagtggccg taaagtattg gacgatgcgg
      181 cagtggcaac tgttaaaaat tggagttttg tgcccgccaa actgggcaat gaaacgatcg
      241 aagcttcagt ggaagtcccc attgattttc gcctcgccaa caactaatca ttttttaaat
      301 taattgagag aatattatgt ctgaattata caaacacatt gtagagtgga ccctgtgggc
      361 gctaattgcc ttttccctgg caacctggac gcttattttg gctaaaagcg tacagcactt
      421 tctaatcggc cgccaaaata gaatttttac aaaagcattc tggagtgccg ccgatttaaa
      481 cgctgcagcc gctttggaaa aatacgtggg ccccactgcg cgcctcgccg ccattgggtt
      541 ttccacttta cacaacgcag atgcgcacac agccagcaac ctagaaacca gttgggatcg
      601 ccaggatttg ctggagcgtc atttacgcca acaaatccaa aaagagcgcc gctctttaga
      661 aagtggttta gccgtattgg cctcagtggg aagtacagcg ccttttgtgg gtctattcgg
      721 taccgtattt ggcattatca tggcgttaac cgcaatttcc agtaaatcgt ccgccagtat
      781 tgaagtagtg gcaggcccca tcggtgaagc gcttgtggcc accggcgtgg gtatcgcagt
      841 agcggttcct gcagtgctgt cgtacaactt ctttttgcgt cgcctaaaat taattgccgc
      901 cgatctggat gattttgcca ctgattttat taccctcgtg caaaaatctg gttttcgtat
      961 tcaggtgcca acaactaata aatctaccca ttcaaaccgc gcagaaaaac ctgctgcaaa
     1021 acttgatgtg gtgcaagagg tactcgcgta atggccattt caaccagcaa cggcgatagc
     1081 gaagaagtat ttggtgagat taacgtaact ccgttggtgg atgttatgtt ggtgttactg
     1141 gtgacattta tcattaccgc ccctatgctc accaatacta ttaatgtcaa tttgcccgaa
     1201 acccaaccga cgctatcgca aaaagaacct gaaaaaccaa ccgttatcag tgtagatgag
     1261 gcaggccagg tgtacgttga caaacagccc tttgccgttg aagccttaga agcagattta
     1321 accgccaaga aaactatcaa ccccgaaata cgccttaact taaatgcaga cgaaaaagta
     1381 ccctatggtg tgattgccaa ggtaatggca gccattgagc gctccggtat
//
LOCUS       DAOQGS010000831         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71206_length_1420_cov_4.768498, whole genome shotgun sequence.
ACCESSION   DAOQGS010000831 DAOQGS010000000
VERSION     DAOQGS010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71206_length_1420_cov_4.768498"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKZ52_19245"
     CDS             complement(<1..240)
                     /locus_tag="PKZ52_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNG61965.1"
                     /translation="MSHFEEISVSQAAEIMAQNKHLWLLDARDAHDFKAGHIDGAMQA
                     HTGLIEHLIKQRDFDRPIIVYCYQGKSSKDIASILG"
     gene            578..796
                     /locus_tag="PKZ52_19250"
     CDS             578..796
                     /locus_tag="PKZ52_19250"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNG61966.1"
                     /translation="MYVCLCHAVTERQVAEAIEKGARCIKDLRAQLNITATCGKCASC
                     ARECIQNKVGKLAQASTQNDLIPMAEAS"
     gene            793..1269
                     /gene="bfr"
                     /locus_tag="PKZ52_19255"
     CDS             793..1269
                     /gene="bfr"
                     /locus_tag="PKZ52_19255"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNG61967.1"
                     /translation="MKGDIKVIEYLNKQLTNELTAINQYFLHARIYKNWGLVKLGKHE
                     YEESIEEMKHADKLIERILFIEGLPNLQMLNRLLIGENVPECLQGDLTLELGSRSVLI
                     EAMAYCETVKDYVSREIFQEILEDTEEHIDYLETQLGLIDKVGIANYLQSQMEPGE"
BASE COUNT          424 a          320 c          306 g          370 t
ORIGIN      
        1 gcccaaaata ctcgctatat ctttgctgct tttgccttgg taacaataca caataatggg
       61 gcgatcaaaa tcccgctgtt taatcagatg ctcaattaaa cccgtatgag cctgcatggc
      121 cccgtctata tgccccgcct taaagtcgtg ggcatcgcgg gcatctaata accacaggtg
      181 tttgttctgc gccatgattt cggcggcttg ggacacacta atttcttcaa agtgactcat
      241 aaacgtctac ccgcaaagta agggaatttc tgattaaaag cgcaggtgta ttcaatctct
      301 taaaatgggt acccatatcc aagctgtacg atgaattgta agctttgcgc caatagatac
      361 taaaagtgca aaactcaatc caagacaacc gggccaatat cctgcgcaaa tcgctctatt
      421 ccacacaacc tagatttggc ctggcgattg ctatcttcac tagaaattga gcgcgattcg
      481 ataaattgtc gcatctacta cagagcgttt gtacattggc caccgctttc aacacgctat
      541 tgatgagtgg cacctgataa aacacgagga ttaggctatg tatgtgtgtt tatgtcatgc
      601 ggtgacagaa cgccaggttg cagaggctat agaaaaaggc gcacgttgca ttaaagattt
      661 gcgtgcacaa ttaaacatca cggccacttg cggcaaatgc gcctcctgcg cccgtgaatg
      721 catacaaaat aaagtaggca aactggccca ggccagcact caaaacgatt taatcccaat
      781 ggcggaggct tcatgaaagg cgacattaaa gttatcgaat atttgaacaa acaacttacc
      841 aatgaattaa ccgcaatcaa tcagtatttt ctccacgccc gtatttataa aaactggggc
      901 ttggttaaac ttggcaaaca tgaatacgaa gaatccatcg aggagatgaa acacgcggat
      961 aaactgattg aacggatttt gtttatcgaa ggcctgccta acctgcagat gctcaaccgc
     1021 ctattgattg gtgaaaatgt acctgagtgc ctacaggggg acttaaccct tgaactgggc
     1081 tcacgcagtg tattaattga ggccatggcc tattgcgaga cggtaaaaga ctatgtttcg
     1141 cgggaaattt ttcaggagat tttggaagat actgaggagc atatcgatta tttagagacc
     1201 cagctgggtt taatcgacaa agtgggcata gcaaattacc tgcaaagcca aatggagccc
     1261 ggtgaataat cttttattcg ccccatttag gtttaaacaa tcgctattat tcagctattt
     1321 ttaaccctaa cagcttgttg aattacgtag gcgacaacgc cctggcgagg ctatatttca
     1381 aaaggctaag gaacctctga aaaattacct tactcgcctt
//
LOCUS       DAOQGS010000832         1420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71208_length_1420_cov_4.682051, whole genome shotgun sequence.
ACCESSION   DAOQGS010000832 DAOQGS010000000
VERSION     DAOQGS010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71208_length_1420_cov_4.682051"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PKZ52_19260"
     CDS             <1..616
                     /locus_tag="PKZ52_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNG61968.1"
                     /translation="GSVESRKVDVRLVAATHRDLGKLAREGKFREDLYYRLNVVQLKL
                     PPLRDRGKDILTLAESFIIRYCHELKRRPLILTPESVQAITSYHWPGNIRELENAIQR
                     AVILCENDQYLDYKLLSIDLDLIHMDEEPNPGTPQRKAPVIPEANEDLSLEDYFQRFV
                     LEHQDSMSETELARKLGVSRKCLWERRQRLGIPRSKGKTVGEGL"
     gene            complement(623..>1420)
                     /locus_tag="PKZ52_19265"
     CDS             complement(623..>1420)
                     /locus_tag="PKZ52_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012487560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNG61969.1"
                     /translation="KYQIEHEDDILEPGDAFAMVNKLLNINQLIGGDPRVEVILLSRN
                     SADTGLRVFNSINHYGLKITRAAFTGGESPYRYIAPFNCQLFLSTDGDDVRRAIDAGF
                     AAATLLASNRSKDASGQLRFAFDGDAVLFSDEAERVYKQKGLDEFARSEREAAKTPLS
                     GGPFKSFLAALQSLQREFDRESCPIRTALVTARSAPAHERVIRTLRAWDIRIDESLFL
                     GGMDKGVFLKAYGADVFFDDQQSHCLSAKDHVATGHVPNGIANLQDG"
BASE COUNT          352 a          423 c          350 g          295 t
ORIGIN      
        1 cggctcagtg gaatcgcgca aagtggatgt acgcctggtg gcggccaccc accgggacct
       61 gggcaaatta gcccgcgaag gcaagttccg tgaagacctc tactaccgtt taaatgtggt
      121 gcaattaaaa ctgccccccc tgcgcgatcg cggcaaagac attctcaccc tggcggaatc
      181 cttcatcatc cgctactgcc acgagcttaa acgccgccca ttgatcctca ccccagaatc
      241 ggtccaagcc atcaccagct accattggcc gggcaatatc cgcgagctgg aaaacgctat
      301 ccagcgtgcc gttatcctct gcgaaaacga tcaatacctg gattacaaac tgctctccat
      361 cgatttagac cttatccata tggacgagga gcctaatcca ggcaccccgc aacgcaaggc
      421 tcccgtcata cccgaagcca acgaggattt atctttggag gattatttcc aacgctttgt
      481 gctggaacat caagattcca tgagcgaaac cgaactggcc cgcaagctcg gcgtcagccg
      541 caaatgttta tgggagcgcc gccagcgcct ggggattccc cgcagcaagg gcaaaacggt
      601 tggcgaaggg ctttagggct tatcagccat cctgcagatt ggcaatgcca ttgggcacat
      661 ggccggtggc cacatggtct ttagccgaca agcagtggct ctgctgatcg tcaaaaaata
      721 catccgcccc ataggctttt aaaaatacgc ccttatccat tccgcctaaa aataacgact
      781 cgtcgatgcg aatatcccag gcgcgcaggg tgcggatcac ccgctcatgg gcgggggcgg
      841 agcgggcggt taccagcgcg gtgcggatgg ggcaggattc ccgatcaaat tcccgctgca
      901 ggctttgtaa agccgccaaa aatgatttaa acgggccgcc acttaagggt gttttagccg
      961 cctcccgctc actgcgggca aattcatcca aacctttttg tttatagacc cgctccgcct
     1021 catcggaaaa caacaccgca tcgccgtcaa aggcaaaacg cagttgcccg ctggcatcct
     1081 tactgcggtt ggaagcgagc aatgtcgccg ctgcaaaccc cgcatcaata gcgcggcgca
     1141 catcatcgcc atcggtggag agaaataact ggcaattaaa cggcgctatg taccgatacg
     1201 ggctctcacc accggtaaag gccgcccggg ttattttaag gccgtagtga ttaatcgaat
     1261 taaatacccg caggccggta tcggcgctgt tgcgggataa caaaataacc tccacccgcg
     1321 ggtcgccgcc gatcagctga ttaatattca ataatttatt caccatagca aaagcatccc
     1381 ccggctccaa aatatcgtcc tcatgttcta tctgatattt
//
LOCUS       DAOQGS010000833         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71471_length_1417_cov_4.477239, whole genome shotgun sequence.
ACCESSION   DAOQGS010000833 DAOQGS010000000
VERSION     DAOQGS010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71471_length_1417_cov_4.477239"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..812
                     /locus_tag="PKZ52_19270"
                     /pseudo
     CDS             1..812
                     /locus_tag="PKZ52_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
BASE COUNT          399 a          294 c          331 g          393 t
ORIGIN      
        1 acttctttaa atatttcgcc ataaattatg aagagcttaa aattaaaaaa ttattgaccg
       61 cttgctatag ggagcggagt ggtgatttat ttggtgagaa aaatcaagat agtggttttt
      121 tcttcgaata caccaataca agcgaagaaa aaagtaagct aactcaacct gaaataaagc
      181 tatttaatgg tgatggagat tttcgcagtt cagagagcat agagctgtta aaaaaatcag
      241 acatcgttgt taccaatccg cctttttcat tattcaggga ttatgttgag cagttggtca
      301 agtatgataa gaaatttcta ataataggaa atgtgaatgc aattacctat aaagaaatct
      361 ttaaacttat taaggagaat aaggcgtggc ttggaataaa ccttggcaga ggtgtatctg
      421 gctttatcgt tcctgaacat tacgaacttt acgggacaga ggctaggatt gatgaagctg
      481 gaaatagaat tgtttcgcct aacaactgtt tatggttaac aaaccttgac acctttaaaa
      541 gacatgaaga tattcttctt acaaagaaat attttgggaa cgagcatgag tatccgaaat
      601 atgataatta cgatggaatc aatgtaaata aaactgaaga tataccgcta gactatgatg
      661 gtatcatggg tgtgcccata acatttctgc acaaatttaa cccagatcag ttcgagctaa
      721 ttaaattcag aaaaggaaat gatgaaaagg atttgtccat caatggcaag tgtccatatt
      781 tcagaatact tatcaaaaat aagcgtgtgc aaattcaatc caattcagaa aacacgtata
      841 acaagtcgtt caactcggac gcgctaaagc gcgccggtta actccaacgt taggcctcaa
      901 tgacgaaccg cgtcacgtac aggtgggcta ttgcttcagt cacgtcgcgc tcaatcattg
      961 cggtgtacgc cgtttgcttc ttggtgggcg cggcgtcgca tgttgttgat ctactgggcg
     1021 ggtggccacc ctatgcaggc tacccagttc cgctccgctt gttctggacg gcattgctac
     1081 cgatcgatgt tgcgcttgct tgggttctgt tctcgagaac agtgccagcg gtggtcatat
     1141 ccgcgcttgt gatgcttctc gatgttgtcg caaataccta tgcaatctac tatctgtggg
     1201 agacggactt cgcgcgcagc cctgggcttt ggcggcagtg cgcctttctt gtcttcatcc
     1261 tgtgtactgc gcctcttcta ctacgggagg caaacgtact tgctcaggca cgagccgggc
     1321 atggcgacca gcaagttgag gcctaaccct tcgctggagc cgactcgcac cggcatggcg
     1381 cttggcccgc gaggcggtgg ttcttatcat ccgcctc
//
LOCUS       DAOQGS010000834         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71637_length_1415_cov_4.099265, whole genome shotgun sequence.
ACCESSION   DAOQGS010000834 DAOQGS010000000
VERSION     DAOQGS010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71637_length_1415_cov_4.099265"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="aroC"
                     /locus_tag="PKZ52_19275"
     CDS             complement(<1..953)
                     /gene="aroC"
                     /locus_tag="PKZ52_19275"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNG61970.1"
                     /translation="MSGNSIGKLFTVTTFGESHGPALGCIIDGCPPGIELSEADLQAD
                     LDRRKPGQSRYTTQRREEDLVEIFSGVFEGKTTGTSIGMMIKNTDQRSKDYGKIKDQF
                     RPAHADYTYLQKYGIRDYRGGGRSSARETAMRVAAGAVAKKVLRERWGIEVRGYLSQL
                     GPIKIEQVDWNEIANNPFFCPDASKVAAMQDYMEALNKEGNSVGAKITVVASNMVPGL
                     GEPVFDRLDADLAHALMSINAVKGVEIGAGFACVEQKGTEHRDEITPQGFLSNNAGGI
                     LGGISTGQDLIAHIALKPTSSLRIPGRSVDINGEPCDIVTTG"
     gene            complement(984..>1415)
                     /locus_tag="PKZ52_19280"
     CDS             complement(984..>1415)
                     /locus_tag="PKZ52_19280"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNG61971.1"
                     /translation="AALDVAADNIQLHQVADRVSLRLSDGFANLVGEQFDLIVSNPPY
                     VDAQDMANLPAEFAREPKLALESGEDGLDFTRILLRQAASHLNDGGHLIVEVGNSWGA
                     LEELFPEISFTWIEFQRGGGGVFIFSKEELQRHQALFDARV"
BASE COUNT          355 a          399 c          325 g          336 t
ORIGIN      
        1 ccggtagtaa caatatcgca gggctcgccg ttaatatcca ccgagcggcc aggaattctt
       61 aagctcgacg tgggcttaag cgcgatatgg gcaattaaat cctgcccggt ggaaataccg
      121 cctaaaattc cgcccgcatt attggacaaa aatccttgcg gcgtaatttc atcgcgatgt
      181 tcagtgccct tttgttcaac acacgcaaac cctgcaccta tctccacgcc tttaacggca
      241 ttaatactca tcagggcatg ggcaagatcg gcgtctaagc gatcaaatac cggctcacct
      301 aaacccggca ccatattact ggccaccaca gtaatttttg cccccacaga attcccttct
      361 ttattgagcg cctccatata gtcttgcatg gcggcaacct tgctggcgtc cgggcaaaaa
      421 aatggattat tggcaatttc attccaatcc acctgctcaa ttttaatcgg ccccaattgg
      481 cttaaataac cccgcacttc aattccccag cgctcgcgca gaactttttt cgccacagcg
      541 ccagcggcca cccgcatagc ggtttcccga gcggatgagc gcccgccgcc gcggtaatcg
      601 cgaataccgt atttttgcag ataggtgtaa tcggcgtggg cggggcgaaa ttgatcttta
      661 attttgccgt aatcctttga gcgctggtcg gtatttttaa tcatcatacc gatggacgtg
      721 cccgtggttt taccctcaaa cacaccggaa aaaatttcca ccaaatcctc ttcccggcgc
      781 tgggtggtgt agcgcgactg gccgggtttg cggcggtcta aatccgcctg taaatccgcc
      841 tcggacaatt caatgccggg cgggcagccg tcaataatgc agccgagcgc tgggccatgg
      901 ctttcgccaa aagtggttac ggtaaataat ttgccgatac tgttgccaga cataaataac
      961 ctagaattaa tctgtattag gaattagacg cgggcatcga acagagcctg atgccgctgc
     1021 aattcttctt tgctgaaaat aaaaacccca ccgccaccgc gttgaaattc aatccaggtg
     1081 aagctgatct caggaaataa ttcttccagc gctccccacg aattgcccac ctcaacaatt
     1141 aaatggccgc catcgtttaa atggctggcg gcttggcgca ataatatgcg ggtaaaatcc
     1201 aaaccatcct cgccggattc caaggcaagc ttgggctcgc gggcgaactc tgccggtaaa
     1261 tttgccatgt cctgagcatc cacatagggc ggattactga cgattaaatc gaactgttcg
     1321 cctacaagat tggcaaaacc atcggataaa cgcaggctga cgcggtcggc cacctggtgt
     1381 aattgaatat tatccgccgc cacatctaaa gccgc
//
LOCUS       DAOQGS010000835         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71716_length_1414_cov_4.570272, whole genome shotgun sequence.
ACCESSION   DAOQGS010000835 DAOQGS010000000
VERSION     DAOQGS010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71716_length_1414_cov_4.570272"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKZ52_19285"
     CDS             complement(<1..1212)
                     /locus_tag="PKZ52_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61972.1"
                     /translation="MPRILSVLICSLLLAACSSKDSGESSGGVKPTSSSSSSSSTGGS
                     SSSTSSSSSSSSSGGLSASSSSSSSSSSSSSSSSSGGPERVSISVEISGPGSASPSSA
                     LVAVGAQLAVNLTPSAGNQLLAAAGCPGVLQGTVFQLGPVTAACSLKVQFGTAAAFTD
                     AALEAAVRKALGFSASRVITASDMAQLSELSVFEGGIKSLQGLEAATNLRNLELTGNP
                     LQDLTPLAGLRRLVTLVIANTQVTSLAALKDVPLRTLNIASTPVSDLAPIASLQSLNR
                     LSMAGTRVIDLEPLRRSSLGAGDYIDMGSSSDGCLYTAGYSRPLSDISVLRGRGVQVY
                     FFDEKMRATGCPNSFAQTQVSLQAEINSPANMSLTWALTSADTGAWRCELHPDLTFQQ
                     PAEPLLRINDCP"
BASE COUNT          305 a          402 c          414 g          293 t
ORIGIN      
        1 ggggcaatca ttaatacgca agaggggttc ggccggttgc tgaaaggtga gatccgggtg
       61 taactcacag cgccaggcgc ctgtgtcggc actggtaagc gcccaggtca ggctcatatt
      121 ggcgggtgag ttaatttccg cctgcaggct cacctgagtt tgtgcaaagc tgttggggca
      181 accggtggca cgcatttttt catcgaaaaa atacacctgc acgcccctgc cccgcaaaac
      241 gcttatgtcg cttaaggggc gggaataacc tgcggtgtac aggcagccat cagagctgct
      301 gcccatgtcg atatagtcgc cagcccccag gcttgagcgg cgcaaaggct ccaggtcgat
      361 cacgcgggtg ccggccatgg acaggcgatt aaggctttgc aggctcgcaa tgggcgccag
      421 atcactcaca ggggtactgg caatattcag ggtgcgtagg ggcacatctt tgagggccgc
      481 aaggctcgtc acttgagtat tggcgataac gagggtaacc agcctgcgca gcccggctaa
      541 gggggtcaag tcttgcagag ggttgccggt aagttcgaga ttgcgcagat tagtggcagc
      601 ctctaagccc tgcaggcttt taatgccgcc ctcaaacacg cttaactcgc tcaattgcgc
      661 catgtcgctg gcggtaatca cccggctggc gctaaaaccc agggctttgc gcaccgccgc
      721 ttccagggcg gcatccgtaa aagccgccgc ggtgccaaat tgcactttta ggctacaggc
      781 agccgttacc ggccccaact gaaacacagt gccctgcaac acacccgggc agcctgcggc
      841 ggcaagtagc tgattgcccg cgcttggggt gagattcacc gccaactgcg cacccacggc
      901 caccagggcc gaactggggg aggcgctgcc ggggccgcta atctccacac tgatgctaac
      961 ccgctccggg ccgcctgaac tgctgctact gctggaggag ctagagcttg aggagctgga
     1021 gctgctcgcg gataagccgc cgctgctact ggaggagcta ctgctcgaag tgctactgga
     1081 agagccgccc gtggatgatg aactgctgga gctgctcgtg ggttttacgc caccactgct
     1141 ttcaccagaa tcttttgagc tacatgccgc cagcaataac gaacatatca acaccgataa
     1201 aattctaggc accgctattc tccaagctac tttgaccgct cagggtatta ccccaggttg
     1261 cgtactgtta ttgcccgtat gctacgcctt tatggatctg gggtacgtgc agagcaaaca
     1321 atttgctgca tctatcacgt ttaacctaaa aaccgctggt gaactcgtag cgagggtgcc
     1381 taaggcgctg aatatgggcg cttttttgtc aaaa
//
LOCUS       DAOQGS010000836         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71812_length_1413_cov_4.846097, whole genome shotgun sequence.
ACCESSION   DAOQGS010000836 DAOQGS010000000
VERSION     DAOQGS010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71812_length_1413_cov_4.846097"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1029
                     /locus_tag="PKZ52_19290"
     CDS             163..1029
                     /locus_tag="PKZ52_19290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNG61973.1"
                     /translation="MNELIQIADADLPTTPIKLVYFDIDGTLLNQHGQYPSQLQSALR
                     RIQNMGVKTAVASGRPQFAAQWLIDDLGLTAPGVFCTGAHIYHPASGAHLQQQFLPLP
                     TLVELVDAMRRLDVYYELYTAEHFYIERDTAAFIRATHAQHLRVAPAVKHFDDIIGAQ
                     PVIKLLIGANQPADIAAVYELERAFSDRCVFAYAKLPAYPDWLFASIIDIKADKQAAF
                     NYLLAHHQLTAEQVMSFGDAQSDKQFLQAAGVGVAMGNAAPDVKAVARFTTKAVWDDG
                     IAYALARLIPDC"
     gene            1170..>1413
                     /locus_tag="PKZ52_19295"
     CDS             1170..>1413
                     /locus_tag="PKZ52_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018014358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HNG61974.1"
                     /translation="MINYTIPKRDMMFVLFELLDYEQHLQSLSGPEALTRDVVEAIVD
                     ECGKFCENVIAPLNSSGDKEGCHWQDGVVTTPKGFKE"
BASE COUNT          337 a          327 c          363 g          386 t
ORIGIN      
        1 cgagtaaggt aatttttcag aggttcctta ggtttatcag ctcttgcact ggtgccatta
       61 tgtctacggt tgtaatggtc agtctaataa atgtttttgt gcgtaaacag ttgagtgcaa
      121 aaaccgccag taatatttcc gatggggtac taaggttttg ctatgaatga attgatccag
      181 attgccgatg ccgatttgcc caccacccca ataaaactgg tgtattttga tattgacggc
      241 accttattaa atcagcacgg ccaataccct agccaactgc aatcggctct gcggcgaatt
      301 cagaatatgg gtgttaaaac tgcagtggcc tccggcaggc cacaatttgc cgcccaatgg
      361 ttaattgatg atttaggttt aactgccccc ggtgtgtttt gcactggcgc ccatatttac
      421 caccccgcct caggagctca tttacagcag cagtttttac cgctacctac actggttgag
      481 ctggtggacg ccatgcggcg gttggatgtg tattacgagc tatatactgc cgagcatttt
      541 tatattgagc gcgataccgc agctttcatt cgcgccaccc atgcacaaca tttgcgagta
      601 gcacccgctg ttaagcattt tgatgacata ataggcgccc agccggtgat taaactgtta
      661 attggtgcca accaacctgc agatatcgcc gctgtatatg agttagagcg cgcgttcagt
      721 gatcgctgtg tatttgccta tgccaaattg cccgcctatc cagattggtt atttgccagc
      781 attatcgata tcaaagcgga taaacaagcg gcatttaatt atctgcttgc gcatcaccaa
      841 ttaactgcag aacaggtgat gagttttggt gatgctcagt cggataaaca atttttgcag
      901 gcggcggggg ttggtgttgc catgggcaat gcggcacctg atgttaaagc tgttgcgcgt
      961 ttcaccacca aagcggtgtg ggacgacggc attgcctatg cgctagcgcg gttgataccg
     1021 gattgctgat cccaggttcg aactgttttc gtgccctata ctcaatgtga aggcaaaggg
     1081 gcccttgtgt tgcagggcgg catttatttt cggcgtgatc aggttttgac cctgtcgttg
     1141 ccacagacgt aaacctagta aggtgaatta tgatcaatta cacaatcccc aagcgcgaca
     1201 tgatgtttgt cctgttcgag ctgctggact acgagcaaca cctgcagagc cttagcggcc
     1261 ctgaggccct aacccgggat gtagtggaag cgattgtgga tgagtgcggt aaattctgtg
     1321 aaaacgtcat agcgccgcta aattcctctg gcgataaaga gggctgccac tggcaggatg
     1381 gtgtagtcac cacgccaaag ggttttaaag agg
//
LOCUS       DAOQGS010000837         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_71826_length_1413_cov_3.465390, whole genome shotgun sequence.
ACCESSION   DAOQGS010000837 DAOQGS010000000
VERSION     DAOQGS010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71826_length_1413_cov_3.465390"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PKZ52_19300"
     CDS             complement(<1..856)
                     /locus_tag="PKZ52_19300"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HNG61975.1"
                     /translation="MTFLHRLTALILTISLIGCTGPGDTASSSSSSGSSSSSTSSSGK
                     TGSSSSSSSSSTSSSSSSSSSSSSSSNSSSSSSSSSSSSGQVQTGWKGKIFIIGDSTV
                     ANYAASAYPQTGWGQVLQPYFDASNVQVVNRAIGGRSSRSFYEEGRFTSVLNEIQKGD
                     YLLIQFGHNDRDFTKAERYTNTTDYKSYLTTYINEARNKGAIPVLVSPMVMNAYNTSG
                     LRNVFTESGNDYRGAMAAVSSELNVAFADLNTKSFNLVKSLGQNYASRYLYLILQAGE
                     YPNFPDGSN"
BASE COUNT          349 a          268 c          403 g          393 t
ORIGIN      
        1 cattgctgcc atcggggaag ttggggtatt caccggcttg taaaattaaa tacagatagc
       61 gggacgcata attttggccg agggatttca caaggttaaa cgatttggta tttaaatccg
      121 caaaggcaac atttaattca ctgctcaccg ccgccatcgc gccgcgataa tcattgccgc
      181 tttcggtaaa tacattgcgc aggccagagg tgttataggc gttcatcacc atggggctta
      241 ctaaaacggg aatagcgcct ttgttgcgcg cttcattgat ataggtggtt aaataacttt
      301 tgtaatcggt ggtgttggtg tagcgctcag ctttagtaaa gtcccgatcg ttgtggccga
      361 attgaatcag taaataatcg cctttttgga tttcatttaa taccgaggta aagcggccct
      421 cctcataaaa actgcgggag ctgcgcccgc cgatggcgcg gttgaccacc tgcacgttgc
      481 tggcatcaaa atagggttgc aacacctggc cccaaccggt ttgcgggtag gcgctggcgg
      541 cgtaattggc cacggtggaa tcgccaataa taaatatctt gcccttccaa cccgtttgca
      601 cttggccgct ggagctggat gaactgcttg aactggacga actgttggat gagctacttg
      661 aactggagga gctactggag cttgatgaag tgcttgagct agaggaggag cttgatgaac
      721 cggttttgcc tgagctggag gttgaactgg agcttgagcc ggagctagag gatgagctgg
      781 cggtgtcgcc cgggcctgtg cagccgatta ggctgatggt caggattaat gcggttaggc
      841 ggtgtaaaaa tgtcatggtt ttatccagtg taagcggcct aggttatggc tattgagccc
      901 gggcctatag caattaatct tttgcaatgc gaaaaatggg gttgtgggct gagcggatga
      961 tactgggatt tatcagattt gattgctaag cagttcgtgg ttttagtatg gatgctgctt
     1021 aggttgcgtt ctgtgctgcg gggtttatgc ggctatacct tcgattactc tacaagacag
     1081 attgtggttt ttcttatgcg aggctgagat tgtgcggtgc tttaagttat ggcgagttct
     1141 tcgatgaaat ttattgaagt gtgactacca ttagctatgg cttgcataaa acggctgagc
     1201 agctataaat ttcaattgct acttgtatgc aatagaaatc aacggtgttc aataggtagg
     1261 agctgggatg aaatacgtaa aatctgaggt gataagccta aagaacagct cagaatataa
     1321 tgaaaaatgg cttcaggccc ggatagaaga ggacccgaca attttggggt tgggagatct
     1381 ggattttcgt gatacggaaa aaattttgat cgg
//
LOCUS       DAOQGS010000838         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_72159_length_1410_cov_3.068635, whole genome shotgun sequence.
ACCESSION   DAOQGS010000838 DAOQGS010000000
VERSION     DAOQGS010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72159_length_1410_cov_3.068635"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PKZ52_19305"
     CDS             <1..1352
                     /locus_tag="PKZ52_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNG61976.1"
                     /translation="AALVFGLALSVASTVVLLRALEASGSLESMNGRIAVGWLIVEDL
                     AMVLVLVLLPALAGLLSDDAGQTQTTTHIGYTLGLTLLKVSAFVVLMLVVGRKVFPAL
                     LWQITRTGSRELFTLSVVAAALTIAWGASALFGVSFALGAFFAGMVLRESEYSHRAAE
                     ESLPLRDAFAVLFFVSVGMLFNPAVLIDEPLRVLAVVAVIMIGKSLAAVALVLALRYP
                     ISAALNVSASLAQIGEFSFILASLGVSLNMLPIEGQNLILAGALISITLNPLLFAGVK
                     PVLKWLVANSKTARVLEHPTDPLAALPSATDAQYLAGQIVLVGYGRVGRRIGESLIAR
                     GIPFIVAEQNREIVERIRATGIAAVAGDAAEPAVLIQAHIARASMLVVSTPDTFDVRQ
                     MIKNARTLNPKIETLVRTHNEEEAEFLEKETAQKVFLGENELANSMTGYILERFGKV"
BASE COUNT          301 a          368 c          389 g          352 t
ORIGIN      
        1 gcgccgcgct agtgtttggc ctggcgcttt cggtcgccag caccgtagta ctgctgcggg
       61 cattggaagc cagcggcagc ctcgaatcca tgaatgggcg catagccgtg ggctggttga
      121 ttgtggaaga cctggccatg gtgctggtac tagtccttct gcctgcgctg gctgggctgc
      181 tcagcgatga cgctggccag acgcagacca ccacccatat cggctatacc ctgggcctta
      241 ccctcttaaa agtctctgcg tttgtggtgc taatgctagt ggtgggccgc aaagtattcc
      301 ccgccctgtt atggcagatc acccgcaccg gctcgcggga attatttacc ctaagcgtgg
      361 tagccgcggc actcaccatc gcctggggcg ccagcgcatt atttggagtg tcctttgcgc
      421 tgggggcatt ttttgcgggt atggtgctgc gtgaatctga atacagccac cgcgccgccg
      481 aagaatcgct gcccctgcgg gacgcctttg cggtattgtt ttttgtatcc gtcggcatgt
      541 tgtttaaccc ggcggtatta attgatgaac ccctgcgggt attagctgtg gtggcagtga
      601 taatgatcgg caaatccctt gccgccgttg ccctggtttt ggccctgcgc taccccatta
      661 gcgcagcctt aaatgtttct gcaagcctcg cgcaaattgg tgaattttct tttattttgg
      721 caagcttggg ggtaagccta aatatgctgc ccatcgaggg gcaaaactta attctcgccg
      781 gcgcgcttat ttctataacc cttaacccac ttttatttgc cggcgttaaa cctgtgctga
      841 aatggctagt ggccaacagc aaaaccgcca gagttttaga gcaccccacc gaccccttgg
      901 cggcattacc ttctgccacc gatgctcagt atctagcagg gcaaattgta ttagtgggtt
      961 acggccgagt cgggcggcgc ataggtgaaa gcttaattgc gcggggcatt ccatttatag
     1021 tggccgaaca aaaccgagaa attgtcgagc gcatccgcgc caccggcata gccgccgttg
     1081 ctggggacgc ggcggaaccg gcggtattaa ttcaagccca tatagcccgg gctagtatgc
     1141 tggtagtcag cactcctgac acctttgatg tgcgccaaat gataaaaaat gcccgcacgc
     1201 ttaaccccaa aatagaaacc ctggtgcgca ctcataatga agaagaagcg gaatttttag
     1261 aaaaggaaac cgcacagaag gtgtttctgg gtgagaatga gctggctaat agtatgactg
     1321 ggtatatttt ggagcggttt ggtaaagttt aaggagtcct ccagaaattc cattatcgtg
     1381 attttcgcaa ggcattcact accgaaatgc
//
LOCUS       DAOQGS010000839         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_72553_length_1406_cov_2.674315, whole genome shotgun sequence.
ACCESSION   DAOQGS010000839 DAOQGS010000000
VERSION     DAOQGS010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72553_length_1406_cov_2.674315"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKZ52_19310"
     CDS             <1..730
                     /locus_tag="PKZ52_19310"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNG61977.1"
                     /translation="EDLGHKYLLVNIPSFELKGVIDNQVVLKKPVIVGRNYRRTPIFS
                     SKINNLIFNPSWTVPPKLAVEDKLPEIQKDPTFFAKLGITVYERDRVVDVAGVNWRRL
                     GKGNFPFRLVQQPGPQNALGQVKFMIPNTDDIYLHDTNTRNLFSRAERAFSSGCIRVH
                     KPLELAEWILGEQGWDMEKIQETIDLGITQTIAVKHPVPVHLEYWTVVLDDEGNVSYR
                     TDLYERDVALWRALRQPITAPTAP"
     gene            730..>1406
                     /locus_tag="PKZ52_19315"
     CDS             730..>1406
                     /locus_tag="PKZ52_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61978.1"
                     /translation="MIQRLTKRLCSALITCSCALLCLPTSHASAATKTAAVQTAKAKS
                     ASNGAKNTKPAAKTSAKRGKTGKKSSRSKQSQSAGAPAAAAKPIFNAAPIAGPKFTTA
                     RASIKVVVNGEDIPYQRFFTTVMPGQPLNISVEGQENYLVNLDGKPLSGQNQRWVWQA
                     PAKPGYHQVQVIEQLTQKTLTLHVFVLAALKNGSDNIAGFTIGRYPPPKDGKAQYKAP
                     LGFIEVNA"
BASE COUNT          369 a          438 c          346 g          253 t
ORIGIN      
        1 ggaagattta ggtcataaat acctactggt taacattccg agctttgaac ttaaaggcgt
       61 catcgataac caagtggtgc tcaaaaaacc ggtcatcgtc ggccgcaact accgccgcac
      121 accgatcttc agcagcaaaa tcaataacct tatcttcaac ccgtcctgga cggtgccgcc
      181 taagctagcg gtggaagaca agcttccgga aattcaaaag gatcccactt ttttcgccaa
      241 actcggcatt accgtttacg aacgtgaccg cgtggttgac gtggccggag tgaactggcg
      301 ccgcctgggc aagggcaatt tccccttccg cctggtgcag cagcccggcc cccaaaatgc
      361 cctagggcaa gtgaaattta tgatccccaa taccgacgat atttatttgc acgataccaa
      421 cacccgcaac ctgttcagcc gggccgaacg cgcctttagt tccggctgta tccgcgtaca
      481 taaaccgctg gaattagccg agtggattct cggcgagcag ggttgggata tggaaaaaat
      541 ccaagagact atagatttag gcatcaccca aaccatcgcg gtgaagcatc cggttcccgt
      601 tcacctggag tattggaccg tagtattgga tgacgagggc aatgtcagtt accgcaccga
      661 cctctatgaa cgcgatgtcg ccctttggcg tgcactgcgc cagcccatta ccgcccctac
      721 agcaccctaa tgattcaacg tctcaccaag cgcctgtgca gcgcactcat cacctgcagc
      781 tgtgcgctgt tgtgtctgcc cacaagccac gccagtgcgg ccactaaaac tgcggcggtg
      841 caaaccgcca aagccaagtc cgccagcaat ggggccaaaa acactaaacc agccgccaaa
      901 accagcgcta aacggggcaa aaccggcaaa aaaagcagcc gcagtaaaca aagccagagc
      961 gcaggcgccc ctgcggcggc ggccaaacct atctttaacg cagcgcccat cgcagggcca
     1021 aagttcacca cggcgcgggc cagtatcaaa gtcgtagtca acggcgaaga catcccctat
     1081 caacgatttt tcaccaccgt catgcccggc caaccgctca acatcagcgt ggaggggcaa
     1141 gagaactacc tagtgaacct cgatggcaag cccttaagcg gccaaaacca gcgctgggtt
     1201 tggcaggcgc ctgccaaacc cggctaccac caggtacagg tgatcgaaca gctgacgcaa
     1261 aaaaccctca ccctgcacgt atttgtactg gccgccctga aaaacggcag cgacaacatc
     1321 gcaggtttca ccatcggccg ctatccgccg cccaaagacg gcaaggcgca gtacaaagcg
     1381 cccttgggtt ttatcgaggt caacgc
//
LOCUS       DAOQGS010000840         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_72896_length_1402_cov_4.467706, whole genome shotgun sequence.
ACCESSION   DAOQGS010000840 DAOQGS010000000
VERSION     DAOQGS010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72896_length_1402_cov_4.467706"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1402)
                     /gene="parC"
                     /locus_tag="PKZ52_19320"
     CDS             complement(<1..>1402)
                     /gene="parC"
                     /locus_tag="PKZ52_19320"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNG61979.1"
                     /translation="LWNREDGDIVITELPHQVSGAKIIEQIAQQMQQKKLPMVADLRD
                     ESDHENPTRLVIIPRSNRIDLDALMNHLFATTDLERSYRINMNYIGLDRRPCVKSLPG
                     MLAEWLVFRTETVRRRLQHRLDWVDRRLHLLDGLLIAFLNLDEVIHIVRTEDHPKEVL
                     MQRFALSEEQANYILDTRLRQLARLEEMKIRGEQAELAKEKADLEATLASEKKLKSLI
                     RKELVQAAKEFGDERRSPIVERAEAQAFSENEILPSEPVTVVLSDKGWIRAAKGHDID
                     PAALSYKAGDSFKYAAQGKSQQNALLLDSTGRSYALPASSLPSARGQGEPLTGKLNPP
                     SGAEFCGLLMGDDNQPVLLASDAGYGFIASFGDLYSKNRAGKTLLSLPEGAKVMPPLP
                     LPATIEGTYLVAVTNEGRMLVFPVSDLPVLPKGKGNKIIGIPSDRLIKREEFLAGLAV
                     ISQEGSVKIFSGKRHLT"
BASE COUNT          321 a          382 c          336 g          363 t
ORIGIN      
        1 ggtgagatgg cgtttgccac tgaatatttt cacgctgccc tcctggctaa ttaccgccag
       61 ccccgctaaa aactcttcgc gtttaattaa tcgatccgaa ggaataccaa taattttgtt
      121 gcctttaccc ttgggtaaaa ccggtaaatc gctcaccggg aataccaaca tgcgtccctc
      181 gttggtgacg gccaccaaat aagtgccttc aatagtggcc ggtaagggca gcggtggcat
      241 taccttggcg ccttccggca aactcaacag cgttttgccc gcgcggtttt tactgtacaa
      301 atcgccgaag ctggcaataa atccgtaccc tgcgtcgctg gctaataaca caggctgatt
      361 atcatcgccc atcagcagcc cgcaaaattc cgcaccggag ggcgggttga gtttgccggt
      421 taaaggctcc ccctgccccc gcgcactggg taggctgctg gcaggcaggg cgtaactgcg
      481 gccagttgaa tctaataaca gagcattttg ctggctttta ccctgggcgg catatttaaa
      541 actatcaccc gctttgtaac tcagtgccgc tggatctata tcgtggcctt tggcggcgcg
      601 aatccagccc ttatcggata acaccaccgt caccggttcg ctgggcagaa tttcattttc
      661 gctaaaggcc tgggcttcgg cgcgctccac aatgggcgag cggcgctcgt cgccaaattc
      721 ttttgccgct tgcaccagct ctttgcggat taatgatttt aattttttct cggaagccaa
      781 ggtcgcttct aaatcagctt tttctttggc aagctcggct tgttcgccgc gaattttcat
      841 ctcttctaaa cgggccaatt ggcgcaaacg cgtatcgaga atgtaattgg cctgctcttc
      901 gctcaaggca aaacgctgca ttaatacttc tttggggtga tcctcggtgc ggactatatg
      961 aatcacttca tccaaattta aaaacgcaat taataaaccg tccaataaat gtaaacggcg
     1021 atccacccaa tctaaccggt gctgcaagcg gcggcgaacc gtttccgtgc ggaacaccaa
     1081 ccactcagcc aacatacccg gcaggctttt cacgcagggc ctgcggtcaa ggcctatgta
     1141 attcatatta atgcggtagc tgcgctctaa atccgtggta gcaaacaggt ggttcattaa
     1201 ggcatctaaa tcgatgcgat tggagcgcgg gataatgact aaacgagtgg gattttcatg
     1261 gtcggattca tcgcgcaaat ccgccaccat gggcaatttt ttctgctgca tttgctgcgc
     1321 gatttgctca ataatttttg ccccgctcac ctggtggggt aattcggtaa tcacgatatc
     1381 gccatcttcc cgattccaca gg
//
LOCUS       DAOQGS010000841         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_72994_length_1401_cov_3.846211, whole genome shotgun sequence.
ACCESSION   DAOQGS010000841 DAOQGS010000000
VERSION     DAOQGS010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72994_length_1401_cov_3.846211"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..861)
                     /locus_tag="PKZ52_19325"
     CDS             complement(169..861)
                     /locus_tag="PKZ52_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNG61980.1"
                     /translation="MTTQEHILVVEDDTSLAEWICDYLGQHNYKVSLATTGTLAVERI
                     ACERPDLVVLDLMLPEKNGFDVCKEVRQFFAKPILMMTASGDEVDEVLGLELGADDYI
                     TKPVKPRVLLARIKALLRREPATGSDSSRSFGRLLIDAQSKSVSLAGNPLDISSHEFD
                     VLWLLACRAGEVVSRTELVSQLRGIEYDGFDRSVDIRISRLRKKLGDSAEAPSKIKTV
                     WGRGYLFAPDAW"
     gene            complement(889..1278)
                     /locus_tag="PKZ52_19330"
     CDS             complement(889..1278)
                     /locus_tag="PKZ52_19330"
                     /inference="COORDINATES: protein motif:HMM:NF022895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3019 domain-containing protein"
                     /protein_id="HNG61981.1"
                     /translation="MRFKRLAASLLLSLSCPLLWAQEDSAATLTAKPNKCIALNRGQA
                     CYQTVRFYWHTPAAGDYCLFAEGQPTPLHCWQGAGAPPFQHEFVGQQSQGFFIRTPEG
                     QTLAQLDLVVAWVYTSGRRDSGGWRLF"
BASE COUNT          281 a          412 c          429 g          279 t
ORIGIN      
        1 atcggggctg tcgagggttt ttgccagttg gcgccccatg cccacgtagg gcgtatccac
       61 ctggccggga tccagctggg aatagacctg ggagatcaac cagcctaggc ccaccacggc
      121 ggttaacacc acggcaataa gtgagagcaa taaaccgcgc acgcgtgctt accaggcatc
      181 cggggcgaat agatagccgc gcccccaaac cgttttgatt ttgctcggcg cctcggcgct
      241 gtcgccgagt tttttgcgca ggcgcgagat gcggatatcc accgagcgat caaagccgtc
      301 gtattcgata ccgcgcaatt ggctgaccag ctcggtgcgg ctgaccactt cccccgcccg
      361 gcaggccaaa agccagagca catcaaattc gtggctgctg atgtctaggg ggttaccggc
      421 aaggcttacg gatttagact gggcatcaat caagaggcgg ccaaagctgc ggctgctgtc
      481 ggagcctgtg gccggttccc ggcgcagcag ggctttgatg cgggcgagta acacgcgggg
      541 tttaaccggt ttggtgatgt agtcgtcggc ccccagctcc aggcccaaca cttcgtccac
      601 ctcatcgccg gaggcggtca tcatgaggat gggtttagca aaaaactgcc gcacctcttt
      661 gcacacatca aagccgtttt tttccggcag cattaaatcc agtaccacca aatccggccg
      721 ctcgcaggcg atgcgctcca ccgccagggt gccggtggtg gccaggctca ctttgtaatt
      781 gtgttggcct aggtagtcgc aaatccattc ggccagggag gtatcgtcct caactaccag
      841 tatgtgttcc tgtgttgtca tgcctgtctc gctgtgaccg atgaggtttt aaaaaagccg
      901 ccagccgccg ctatcccggc gcccggaggt gtacacccag gccaccacca gatccagctg
      961 cgccagggtt tggccctcgg gcgtgcgaat gaaaaacccc tggctttgtt ggccgacaaa
     1021 ttcgtgttgg aacggcggtg cacccgcgcc ctgccagcag tgcaggggtg tcggttggcc
     1081 ctcggcaaac aggcaatagt ccccagcggc cggggtgtgc cagtagaagc gaaccgtttg
     1141 gtaacaggcc tggcccctgt tgagggcaat gcatttattg ggtttggctg taagggtggc
     1201 agccgaatcc tcctgcgccc agagcagagg gcagctaagg ctgagcagca ggcttgcggc
     1261 taagcgctta aaacgcataa ctcaccgtag ccgccagcac cagggcgtgg ttttcatcga
     1321 ttagcgggct gcgggcgaca ttatccggca gatcggaata acgcagatag gtgcgcccca
     1381 cccagtggtc actgatgggg t
//
LOCUS       DAOQGS010000842         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_73553_length_1395_cov_5.300000, whole genome shotgun sequence.
ACCESSION   DAOQGS010000842 DAOQGS010000000
VERSION     DAOQGS010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73553_length_1395_cov_5.300000"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(583..>1395)
                     /locus_tag="PKZ52_19335"
     CDS             complement(583..>1395)
                     /locus_tag="PKZ52_19335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNG61982.1"
                     /translation="RRGFIKEAAKYTASTLAATALLDSLAPNFAEAQQVTPGDKRLKG
                     EYVEYPSPNGNGKIRGYLVKPAKAKGKLPSVLVVHENRGLNPHIEDIARRLALDNFIA
                     FAPDALFTLGGYPGDEDKARELFQKLDQTKTQEDFVTAADILKKLPGSNGKVGVVGFC
                     YGGGIANFLATRVPTLNGAVPFYGRQPEEADVAKIKAPLLIHYAGTDERINAGWPKYE
                     AALKAAKTQYEAFIYPNVQHGFNNDTTPRYDAAAAALAWGRTVTFFNKHLRA"
BASE COUNT          372 a          315 c          351 g          357 t
ORIGIN      
        1 gaaggtcatt ttgccatctg gcgcgggggt tttacacaat tctacgaaga gccaagtatg
       61 tagccaggcc atagactggc gaaaaccatt tttcattggg tacctcccaa gcttaaaaat
      121 ataaaattaa ttacagccaa aattaacgtg ggtgataaaa gacctattgt ggctcgccgc
      181 aagctgtgcg ccgaaaaaac ccaaataact gcgagtgtgt agataacaaa agttgacagg
      241 gccgaggtga cgacggcatc actgcggctg aggggcaaca cattgaccag aaatatagcc
      301 gtaactacag tggcaaaata accacccacc gccgcaagca atactcgtag ggcaacctgc
      361 aggcggtagg ataaaagtag tgattgtgag gttgcttttg ccatatcagt taattaccaa
      421 agtacgccaa ggctaaagta gaaaatttaa ttaaattcca agggttgatg tgggaataca
      481 ttgtttattt aatgccgtta gcaaaacggt gtgcaagcac gcttgcacac cctacagctt
      541 gttgcccacc tttttgctag tcgaactgtg agtcaaactc gattaggcgc gcaaatgttt
      601 attaaaaaat gtcacagtgc gcccccaggc aagcgctgca gcggcggcat catagcgggg
      661 cgtggtgtcg ttgttaaaac catgttgtac atttgggtaa ataaatgctt cgtactgggt
      721 tttagccgct tttaaagccg cttcgtattt gggccagcca gcattaatgc gctcatcagt
      781 gccggcgtaa tgaattaata agggtgcttt aattttggct acgtcagcct cttcaggctg
      841 tctgccataa aaaggtactg caccattcaa ggtggggacg cgcgtagcga ggaaattggc
      901 aatgccgccg ccgtaacaaa aacccaccac gcccaccttg ccgttactgc caggcagctt
      961 ttttaatata tcggctgcgg taacgaaatc ttcttgggtt ttggtttggt caagtttttg
     1021 aaataattcg cgggctttgt cctcatcccc tggatagccg ccaagggtaa ataaggcgtc
     1081 tggcgcaaag gcgataaaat tatccaatgc taagcggcgg gctatgtctt cgatatgcgg
     1141 attgaggcca cggttttcgt gcacaaccaa tacgctgggc aatttacctt tagccttggc
     1201 aggtttaacc aaataaccgc gaattttgcc attgccgttg ggtgagggat actcaacata
     1261 ttcgcccttg aggcgtttgt caccgggtgt aacctgttgc gcctctgcaa aattcggtgc
     1321 taaggaatcc agcagagcag ttgctgctag ggtgctggcg gtatattttg ccgcttcttt
     1381 aataaaaccg cggcg
//
LOCUS       DAOQGS010000843         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_73677_length_1394_cov_3.433159, whole genome shotgun sequence.
ACCESSION   DAOQGS010000843 DAOQGS010000000
VERSION     DAOQGS010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73677_length_1394_cov_3.433159"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1224
                     /locus_tag="PKZ52_19340"
     CDS             43..1224
                     /locus_tag="PKZ52_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNG61983.1"
                     /translation="MLSLAQAQATLWQQAVPVAQIIWLPLQQALGARAAQAVLAQLAV
                     PPADNSAMDGYALRLSDATEPLPVSARIAAGCAPEPLAPGCAARIFTGGEIPPGADTV
                     VMQENTECLPDGRVRITVPPKLGANIRPMGQDISPGQLLVAAGERLGPVALALLASQG
                     ITQVEVLRPVKVALVATGSELVAPGQALGAGQIYNSNSQLLGSALSLLGAQVHHYSVA
                     DDAAQTQALLAELAAGMDVIVSTGGVSAGEEDHVKASLAALGQVSFWKIALKPGKPFM
                     FGQIGATPVLGLPGNPASSFITYCLLAKPFIQALQGGPARLPPPRLVPVACSLSAGSR
                     EEFVRVEWVEAGVRPLANQSSGALRSLSLADGVVRLAANQSLAAGEAVEYWTLDELLA
                     P"
BASE COUNT          236 a          420 c          473 g          265 t
ORIGIN      
        1 ccgcaccaag ccctgtaatt ttgtcgcggt gttaaagccc tcatgttaag cctggcgcag
       61 gcccaagcaa ccctgtggca acaggcggtg ccggttgccc agatcatttg gctgccgctg
      121 cagcaagcct tgggcgcgcg ggcggcgcag gcggtactgg cgcagcttgc ggtgccgcct
      181 gccgataaca gtgctatgga cggctacgcc ctgcgcttaa gcgatgcgac ggagccctta
      241 cccgtgtctg cgcgcattgc ggcgggctgc gcgcccgagc ctttagcccc aggctgtgcg
      301 gcgcgcattt ttaccggcgg ggaaattccg ccgggggccg atacggtggt gatgcaggag
      361 aacaccgagt gcttgcccga tgggcgggtg cggataaccg tgccgcccaa actcggcgcc
      421 aatattcgcc ccatgggcca ggatataagc cccggccaac ttttagtggc ggcgggggag
      481 cggctggggc cggtggctct ggcgctgctg gcctcccagg gcataaccca ggtggaggtg
      541 ctgcgcccgg tgaaggtggc gctggtggcc accggctcgg agctggtggc gcccggccaa
      601 gccctcggcg ccgggcaaat ctacaacagc aatagccagt tgctcggcag tgccctcagc
      661 ctgttgggtg cccaggtgca ccattactcg gtggcagatg acgcggcgca aacgcaagcc
      721 ttgctggctg aacttgccgc cggtatggat gtgattgtca gcaccggggg cgtgtcggcg
      781 ggggaggagg atcacgttaa ggcgagcctt gctgcactcg ggcaggtgag cttttggaaa
      841 atcgccctca aaccgggtaa accctttatg ttcggccaga ttggcgccac cccagtgctg
      901 gggttgccgg gcaacccagc gtcgagtttt atcacctatt gcctgttggc taagcctttt
      961 attcaagccc tgcagggcgg cccggcccgc ctgccgccgc cgcgcctggt gcctgtggcc
     1021 tgcagcctca gcgcaggcag ccgcgaagag tttgtgcggg tggaatgggt tgaggcgggt
     1081 gtacgcccgc tggccaacca aagttccggt gcactgcgct cgttgagcct ggccgatggc
     1141 gtagtccgtt tggcggccaa ccaatcgctg gcagcgggag aggcggtgga atactggact
     1201 cttgatgagt tgttagcgcc atgagcggcc ccctgctgga tcggatcgat attcatgtcg
     1261 aagttccgcc cgttcctttt cgagaactct ccaatgataa agtcgggacc aacagccagt
     1321 cgatgcggga atgcgtgatc gctgcccgcg atcgacagaa acaccgattc cgcgagactc
     1381 cgggagcact caat
//
LOCUS       DAOQGS010000844         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_73692_length_1394_cov_3.031367, whole genome shotgun sequence.
ACCESSION   DAOQGS010000844 DAOQGS010000000
VERSION     DAOQGS010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73692_length_1394_cov_3.031367"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..>1394
                     /locus_tag="PKZ52_19345"
     CDS             296..>1394
                     /locus_tag="PKZ52_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNG61984.1"
                     /translation="MSRIIAALAERMAQQEALLARPNEVAAGNGVFSRWRNPILTREH
                     TPLFWRYDLNPASNPQGLERLGINATFNSGALFWQGQYTLVVRVEGNDRKSFFAVAQS
                     PNGVDNFRFWDFPIDLPQTEAPDVNVYDMRLTQHEDGYIYGLFCTERKDPAQPGDSSA
                     ATAQCGIVRTKDLVRWERLPDLVTYSGQQRNVVLHPEFINGQYGLYTRPQDGFIAVGA
                     GGGIGWGLTPSMEAAQLQREIILDPKHYHTVQEVKNGQGPPPLKTSAGWLHLAHGVRA
                     TAAGLRYVLYVFLTDLQEPWRVLYKPAGHFLAPLGPERVGDVSNVVFSNGWISNAAGE
                     VFIYYAASDTRMHVATTSIERLLDYVHNTPPD"
BASE COUNT          269 a          399 c          433 g          293 t
ORIGIN      
        1 atagcgatgc cccgccacca ctttttagcc tcccaaaggc ggtgtggtta aggcggaacc
       61 tggggcaacc cttgtattgc ccggtgcgtg tctcggctat agatcaggat ctgcgcggca
      121 gcgggcaccg gagcgcccct atggctgttg gctgtggggg aaatgcttaa cctgatgcca
      181 tcgcctgcga cgcttaggaa ttttggccct ggctatggcg aaaaatggcg gtcaactctt
      241 aacttaatga cattggcttg gcggcgaatg tccgttttca aaaggtaaat ggtttatgtc
      301 cagaataatt gccgccctag cggagcggat ggcccagcag gaggctttgc ttgcgcgccc
      361 gaatgaggtt gctgcgggca atggggtgtt cagccgctgg cgcaacccca ttctcacccg
      421 cgagcacacg ccgctgttct ggcgctacga cctcaacccc gccagcaacc cgcagggctt
      481 agagcggctg ggtatcaatg ccacctttaa ctccggcgcg ctgttctggc aggggcaata
      541 caccttagtg gtgcgggtgg aggggaatga tcgcaaatcc ttttttgcgg tggcccaaag
      601 tcccaatggg gtggataact ttcgcttctg ggattttccc atcgacctgc cgcaaaccga
      661 ggcgcccgat gtcaatgttt acgacatgcg cctgacccag catgaagacg gttacatcta
      721 cggtttattc tgcaccgagc gcaaagaccc ggcgcagccg ggcgatagct cggcggctac
      781 ggcccagtgc ggcatagtgc gcaccaagga tttagtgcgc tgggagcggc tccctgatct
      841 cgttacctat tcggggcagc agcgcaatgt tgtgttacat cccgaattca ttaatgggca
      901 atacggcctc tacacccgcc cgcaggacgg ctttatcgcc gtgggggccg gcggcggcat
      961 cggctggggg ctgacgccga gcatggaggc ggcccagctg cagcgggaaa ttatcctcga
     1021 ccccaagcac taccacacgg tgcaggaagt gaaaaacggc caggggccgc cgcctttaaa
     1081 aacctctgca ggctggctgc atttggccca tggggtgcgc gccaccgcag cgggtttgcg
     1141 ctatgtgctg tacgtgtttc tcaccgatct tcaagaaccc tggcgggtgt tgtacaaacc
     1201 cgccgggcat tttctggcgc ccctggggcc tgagcgggtg ggggatgtct cgaatgtggt
     1261 gttcagcaat ggctggataa gtaacgcggc gggggaggtg tttatctatt acgccgcctc
     1321 agacacccgt atgcacgtgg cgaccacgag catcgagcgg ctactcgatt atgtccacaa
     1381 cacgccgccg gatg
//
LOCUS       DAOQGS010000845         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_73939_length_1391_cov_6.325599, whole genome shotgun sequence.
ACCESSION   DAOQGS010000845 DAOQGS010000000
VERSION     DAOQGS010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73939_length_1391_cov_6.325599"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PKZ52_19350"
     CDS             complement(<1..>1391)
                     /locus_tag="PKZ52_19350"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HNG61985.1"
                     /translation="VVLAWLLGQSTNLEDVLVGHLLSGVLLNNSACPLMQALETTELG
                     QSPSPLCGMDDSQKELTFICGLEGATAENIEAIEAFILTTLEKIASEGIPQEDAEAAL
                     HQLELHQREVGGDHHPFGLQIILNALTAATHRGDPVALLNIDPALSKLREQIKDPAFL
                     QGRIRALLLDNPHRVRLALCPDAHLAERKQAAEVAQLARIRAALNDREKQAILEQAEA
                     LLQRQGRVDDENILPKVTLEDVPLKEGHLSPSVLSLHTDNDLYRYDVGTNGLVYQQIV
                     LDLPQLAPEQINLLPYYTNLLTEVGVDQHDYIAMQRKQAAATGGFSAYTSIRGLVDDV
                     HSSQNFLTLSGKALNRNQAALSQIMQATLSGARFDELNRIRESMAQARAHREQSITNN
                     GHGLAMTAATQGLCAIARLNHELGGLASIQRLKQLDDSLDDTSALSDFCAQLAGIHHT
                     IKNAPRRFLWVGEA"
BASE COUNT          244 a          351 c          464 g          332 t
ORIGIN      
        1 gcttccccca cccacaaaaa gcgccggggg gcatttttga tggtgtggtg gatgccagcc
       61 agttgggcgc aaaaatcgct tagggcgctg gtatcatcca ggctgtcgtc gagttgtttg
      121 aggcgctgga tggaggcgag gccgcccaac tcgtggttga ggcgagcgat ggcgcacagg
      181 ccctgggtgg ctgcggtcat ggcgaggcca tggccgttat tggtgatgga ttgctcccga
      241 tgggcgcggg cttgggccat gctttcgcgg atgcggttga gttcatcaaa gcgggcgccg
      301 cttagagtgg cctgcatgat ttggctcagc gctgcctggt tgcggttgag ggctttgccg
      361 gagagggtta aaaaattctg gctgctgtgc acatcatcca ccaggccgcg aatactggtg
      421 taggcgctaa aaccgccggt ggccgccgcc tgcttgcgtt gcatggcgat gtagtcgtgc
      481 tgatcaacac ccacctcagt taacaggttg gtgtagtagg gcagcaggtt aatttgctcc
      541 ggggccagtt gcggtaagtc caacacaatc tgttggtaca ccaggccgtt ggtgcccaca
      601 tcgtagcgat agaggtcgtt atctgtgtgc aggctgagta cgcttgggga gagatggccc
      661 tctttcaggg gcacatcttc cagagttact ttgggcagga tattttcatc atccacgcgg
      721 ccttggcgct gtaaaagggc ctcggcctgt tcgagtatgg cttgtttttc acggtcattt
      781 aaagcggcgc ggatgcgggc cagttgcgcc acctcggcag cctgtttgcg ctcggcaagg
      841 tgtgcatcgg ggcatagggc caggcgtacc cggtgggggt tatccagcag tagggcgcgg
      901 atgcggcctt gtaaaaaggc tgggtctttt atttgttccc gcagtttgct cagggccggg
      961 tcgatattga gtagggccac cgggtcgccg cgatgggtgg cggcggtgag ggcgttgagg
     1021 ataatttgca gcccaaaggg gtggtgatcg ccgcccactt cccgctggtg taattccaac
     1081 tgatgcaggg ccgcctcggc atcctcttgg ggtatgccct cgctggcgat tttctctagg
     1141 gtggtgagaa taaacgcctc aatggcttct atgttctctg ccgtggcgcc ctctaggccg
     1201 caaataaagg tcagctcttt ttgtgaatca tccatgccgc acagggggct ggggctttgg
     1261 cctaattcgg tggtttccaa cgcttgcatc agtgggcagg cgctgttgtt gagcagtacg
     1321 ccagagagca aatggcccac taatacatct tctaaattgg tggattggcc cagcagccag
     1381 gcgagcacca c
//
LOCUS       DAOQGS010000846         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_74429_length_1386_cov_3.284748, whole genome shotgun sequence.
ACCESSION   DAOQGS010000846 DAOQGS010000000
VERSION     DAOQGS010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74429_length_1386_cov_3.284748"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKZ52_19355"
     CDS             complement(<1..564)
                     /locus_tag="PKZ52_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018274762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61986.1"
                     /translation="MGLRKQTIFLSLISTLASPWVMGLGLGEIKLKSTLNQPLYAEIQ
                     LTQTKDLTDREILVGLASAADFARVGVERPGFLGDLKFQVVMDPGKGPLVRITSSKAV
                     VEPYLNFIVQAQWPSGKLLREYTVLVDLPTYGGDEPASAAPPVSRPSTNVPKVETQLT
                     QAPEAPRARVNRPAAAAATPRPKVPRPA"
     gene            complement(1054..>1386)
                     /locus_tag="PKZ52_19360"
     CDS             complement(1054..>1386)
                     /locus_tag="PKZ52_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNG61987.1"
                     /translation="IDGLCVRIGAMRCHSQAITLKLNKNVPMADIEQLIAQANDWVTL
                     VPNTREASLQDLTPTKVTGTLSIPVGRLRKMNMGPEYLSAFTVGDQLLWGAAEPLRRM
                     LRILLAHV"
BASE COUNT          311 a          376 c          391 g          308 t
ORIGIN      
        1 cgctgggcgc ggtactttcg ggcgaggcgt tgccgctgcg gccgccggac gattgacccg
       61 cgcgcggggc gcctcggggg cttgggtcag ctgggtttca accttgggga cattagtgct
      121 ggggcggctg accggtggag ctgcgctggc aggctcatca ccgccatagg tgggcaaatc
      181 taccagcacg gtgtattcgc gcagcaattt accgctgggc cactgggctt gcacaataaa
      241 atttaaatag ggttcaacaa ctgctttgct ggaggtgatg cgcaccaggg ggcctttgcc
      301 tgggtccatc accacttgga atttaagatc gccgaggaac cccgggcgct caacacccac
      361 acgggcaaag tctgcggcgg aggccagccc taccagaatc tcccgatcgg taagatcctt
      421 agtctgcgtc agttgaatct ccgcatagag tggctggtta agagtcgatt tcagcttgat
      481 ttcacccagg ccgaggccca tcacccaggg cgaggccagg gttgaaatta agcttaaaaa
      541 gatcgtttgt tttcgcagac ccataagggt aatccttatt gttttaggag gaagactcac
      601 ctgtttggat aaccccgcaa acccagagcg acacgacagc cgatcaacaa gactctcttg
      661 actgaagggt attcgaccgc acggcttaca gacactaatt cttattatgc taatgctggg
      721 caacaccccg agattcagtt taaaaccgaa tgccccgtga agttgcccat cttattcgct
      781 tatcagcgta agtattcatg acaaacggtg ttttttcaac ttgcgcgcca aatgtcagtc
      841 accaagcgcc gcctggcgga aaattccctt tgcgcatggg gcgacagtgc ttagcgcgct
      901 atgcattgaa tagtgcaatg tttcggcttt tatatgggtg ttttgacggg tggacgcgtt
      961 ttgcgggaaa gacgcaagcg agcatccctg cccactcgaa tgctctggcc agcagttgcc
     1021 agccagatga ggcccttgcg ggcggcgatc agatcagacg tgagccaata aaatgcgcag
     1081 catacggcgc agaggttcag cagcgcccca cagcagctgg tcccccaccg tgaaggcact
     1141 taaatactca ggccccatat tcatcttgcg cagacggcca acgggaatgc tcaaggtacc
     1201 ggtaaccttg gtcggtgtta aatcctgcag tgaggcctcg cgggtattgg gaaccagggt
     1261 tacccaatcg ttggcctggg caattaactg ctcaatatcc gccatgggga catttttatt
     1321 cagcttcaat gtgatcgcct ggctgtggca gcgcatggcg ccgatgcgca cacacaggcc
     1381 atcaat
//
LOCUS       DAOQGS010000847         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_75226_length_1378_cov_6.232048, whole genome shotgun sequence.
ACCESSION   DAOQGS010000847 DAOQGS010000000
VERSION     DAOQGS010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75226_length_1378_cov_6.232048"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKZ52_19365"
     CDS             complement(<1..414)
                     /locus_tag="PKZ52_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNG61988.1"
                     /translation="MSRILMGALLVLSSQAFAEKFSLSSPSIKEGQTLSLEQVFNSFG
                     CSGKNISPALSWAHPPAGTKSYALTVYDPDAPTGSGWWHWVVYNLPATATGLPAEAGD
                     ASGKNLPAGAVQGRTDFGSTGFGGACPPPGDKPHRY"
     gene            550..1302
                     /locus_tag="PKZ52_19370"
     CDS             550..1302
                     /locus_tag="PKZ52_19370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNG61989.1"
                     /translation="MANCHLYEDLSAYYDRFCQDIPYAQQAATLKRLVQLFNDSPGEH
                     YLDIACGTGQLMEHMQHYGWQLAGLDNSAAMLKQAAQRCPEATWLLADMAKLPAQPQV
                     NFASCLLYSMHYNSDIEQLTVFFRAVWDALVPGGFLVFDCVDKRGIDNSAGITTYYQD
                     GEQQFTFQSRWAYSGEGNQQSLQLSIELNQNGRISAWQDEHPMVAVSSVELEAMLAAQ
                     GFEVTLFERNFEQLIAWQGASFNLMVVAQKPV"
BASE COUNT          307 a          315 c          394 g          362 t
ORIGIN      
        1 atagcggtgg ggtttatcgc cggggggtgg gcaggcgccg ccaaacccgg tgctgccaaa
       61 atccgtgcgg ccttgcactg cgcctgcagg caggttttta ccgctggcat cgcctgcttc
      121 cgctggcagg cccgtggcgg tggcgggcag gttatacacc acccaatgcc accagccgga
      181 gccggtgggg gcgtctgggt cgtacacggt aagggcataa cttttagtgc ccgccggtgg
      241 gtgggcccag ctaagcgcag gggaaatatt tttaccgctg cagccaaaac tattaaatac
      301 ctgctccagg ctgagggttt gcccctcttt aatgctgggg ctggataagc tgaatttttc
      361 ggcaaatgcc tggctgctga gtactaataa agcacccatt aagattcgcg acatagcagt
      421 tcctctgtga tgtggttgtg gtggcggcaa gtgtacctgg tacggccatt attgccagct
      481 gggtttatgt gtggggttgt ttttttaaat ccagtggctc tgtcattaaa tattatgtga
      541 ggattgtcgg tggcaaactg ccatttatac gaagatttat cggcctatta cgaccgtttt
      601 tgccaggata ttccctatgc ccagcaggcc gcaacactaa agcggctggt gcaattattt
      661 aatgactccc ctggtgagca ttatttggat atcgcctgcg gcaccggcca attaatggaa
      721 catatgcaac attatggatg gcagttggcg ggtttagata attccgccgc catgctcaaa
      781 caagcggccc aacgctgccc cgaggcgaca tggttgttgg ccgatatggc taaattaccg
      841 gcccagccgc aggttaattt tgccagctgc ctgttgtatt ccatgcatta caacagcgat
      901 atagagcaat taacggtatt ttttcgtgcg gtgtgggatg ctttagtgcc cggtggtttt
      961 ttagtatttg attgtgtgga taaacgcggc attgataatt cggcgggtat taccacttat
     1021 tatcaagacg gcgagcagca atttaccttt caatcccgct gggcctatag cggtgaaggc
     1081 aatcaacaaa gcctgcaatt gtccattgag ctgaaccaaa atggccgcat cagcgcctgg
     1141 caggatgaac accccatggt ggcagtaagc agtgtagagc tggaagcgat gctggctgca
     1201 caagggtttg aggtgacttt gtttgagcgc aattttgagc aattaattgc ctggcagggg
     1261 gcgagtttta atttaatggt ggtggcgcaa aagccggtgt gatagttgcc tgaagcagaa
     1321 atgttaggga acctctgaaa aattacctta ctcgctctgg cgtcgttgaa tttgccaa
//
LOCUS       DAOQGS010000848         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_75327_length_1377_cov_5.295008, whole genome shotgun sequence.
ACCESSION   DAOQGS010000848 DAOQGS010000000
VERSION     DAOQGS010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75327_length_1377_cov_5.295008"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PKZ52_19375"
     CDS             <1..1163
                     /locus_tag="PKZ52_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61990.1"
                     /translation="EPFELLYPVTPILNQAIGFEVVFELHNLTTFRWVVQLTNRLDFT
                     VGLIVQAAGWQAWITLAAGQTRYLSGATAVSGMGNWNAYPLHPFSPVYGWHRNWSNEF
                     MRMLDGECQVSFRLYLTAPDQADCIPPGLPALQAGLRWQFNPTDLEMRFIPRWSAIKR
                     FSRTGVYEYDFNNADDPILERTPAIRLPAYSMLNPATYFAQGYDTAPGDTSPWHKRPW
                     SQAQQVSLMCRGEKLSTEGFETLWVAAEDIALLIQHEHLAWVAAHLGVTDAVIMDGND
                     IRWFEIGPNNTWHNLLWINRFYTLAGIAVDEAAMRAAFNAMLAHFNDDWPTYYRTLSV
                     HEAVRFGTFLDNGAAEMGDYAVNETGHFLEANNLDVNAVSTRFLYRIKPFTG"
BASE COUNT          287 a          367 c          349 g          374 t
ORIGIN      
        1 tggagccgtt tgagctgctc tacccggtca cacccatcct caaccaggcg attggctttg
       61 aggtggtatt tgagctacac aatctcacca cctttcgctg ggtggtccaa ctgaccaacc
      121 gtctggattt tacggtggga ctgatcgtgc aagcggcggg ttggcaggcc tggataaccc
      181 tggccgccgg gcaaactcgg tatttgagtg gcgccacagc ggtgtccggc atgggcaact
      241 ggaatgccta cccactgcac ccgttttcgc ccgtctatgg ctggcaccga aactggagca
      301 acgagtttat gcgcatgcta gacggggaat gtcaggtgtc gtttcggcta taccttacgg
      361 caccggatca ggccgattgt attccgccgg gattaccggc tctgcaagcg ggtctgcgct
      421 ggcaatttaa ccccacggat ttagaaatgc gctttattcc gcgctggtca gccatcaaac
      481 ggttttcacg cacaggcgtg tatgaatacg attttaacaa tgccgatgac ccgatcctgg
      541 agcgaacgcc cgccatccgc ctgcccgcct actcaatgct aaatcccgct acttattttg
      601 cccaaggcta cgacaccgca ccgggtgata ccagcccctg gcataagcgg ccctggtccc
      661 aggcccagca ggtgtccctc atgtgccgag gggaaaaact cagtaccgag ggatttgaaa
      721 ccctttgggt ggcggctgag gatattgccc tgctcatcca acacgagcat ctggcctggg
      781 tcgctgcgca cctgggggtc accgacgctg tgatcatgga tggtaacgat atccgctggt
      841 ttgaaatcgg accgaataac acctggcaca acctgttgtg gattaaccgt ttttataccc
      901 ttgctggaat tgccgtcgat gaagcggcca tgcgcgcggc gtttaacgcc atgctcgccc
      961 acttcaatga cgattggccg acctattacc gcactttaag tgttcatgaa gccgtccgtt
     1021 ttggcacttt tttagataat ggcgctgcgg aaatgggtga ctatgcagtg aatgaaacgg
     1081 gccatttttt ggaggcgaac aacttggatg tcaatgccgt gagcacacgt tttttgtatc
     1141 gcatcaaacc cttcactggc tgaaaacgat ttctattttt ttatttttat tcttactttt
     1201 aaattttagt ttgattttta atattcaatc gccggtttat tttcgcttta attctcgtgt
     1261 ttattttttt accgcctgat ttcgttgcca atttttcccc actaccgctt tatcgaactt
     1321 agacatgatc cagttagacg gccttgtttt acccagcggg ttacgctgga gtgatga
//
LOCUS       DAOQGS010000849         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_75557_length_1375_cov_4.873485, whole genome shotgun sequence.
ACCESSION   DAOQGS010000849 DAOQGS010000000
VERSION     DAOQGS010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75557_length_1375_cov_4.873485"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..545)
                     /locus_tag="PKZ52_19380"
     CDS             complement(60..545)
                     /locus_tag="PKZ52_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61991.1"
                     /translation="MAQPNLINLIIPAADLQDIQTALNTLTSKLAPHLISLSANERKE
                     IPKMGDKTQAFVGKAREYASQNPDLVPNYLDVGAFEVDWQAVELLSRLQRQLAPLADN
                     LSDSLALSGSEAYQAALIFYRNIKMAARAGVPNAKTIEADLSNRFPGSSARASTSAEP
                     A"
     gene            complement(1102..>1375)
                     /locus_tag="PKZ52_19385"
     CDS             complement(1102..>1375)
                     /locus_tag="PKZ52_19385"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNG61992.1"
                     /translation="AYSPVTIWVRDGKISQVISGVQVLRVEDYQQASIEQIFARIAYT
                     LSHSPAQTQFFMEFDPLLGFPSQFKATSSSYAGGTQLVVTDLTIDP"
BASE COUNT          275 a          316 c          443 g          341 t
ORIGIN      
        1 gggtaaagca ctttgtagca gggggcaggt ggtgccccct gccccgctgc agcctagact
       61 taggccggtt cggcgctagt gctggccctg gcgctgctgc cggggaagcg gttggagagg
      121 tccgcttcga tggttttggc gttgggcacg cccgcgcggg cggccatttt tatattgcgg
      181 tagaagatca gggcggcttg gtaggcttcg ctgccgctga gggccaggct gtcgcttagg
      241 ttgtccgcca ggggtgccag ttggcgctgc agacgggaca ataattccac cgcctgccag
      301 tccacctcaa aggcacctac gtccaaatag ttgggcacca aatctgggtt ttggctggcg
      361 tattcccgcg ccttacctac aaaggcttgg gttttatcgc ccattttggg aatttctttg
      421 cgctcgttgg cgctgaggct aatcaggtga ggggcgagtt tgctggtgag ggtgttgagt
      481 gcggtttgaa tgtcttgcag atccgcagcg gggataatta agttaattaa attaggttgc
      541 gccatggtta aatccctgtg gttgttaatt aagtgctgtg cagcgttttt taataccgcc
      601 gcctgcgcag tgttacagag ctatttatag ctgtgaagag gtgattgccg cttgtggctg
      661 tgcgctatgg cgcgcaaaca gccttgggga attaatttaa aaaaacggct tgttggccgg
      721 gcagttttgt tagctgtggg aatggggcaa tttcattggg ttgagcattt tgtagtgttt
      781 tttggctgat gtttgcggtt agccctaaac cccaccccag ccctcccctt aacaagggga
      841 gggagtaaaa atgtttggag cacaaacaat atgccccagc tggaagattt ttggcccctc
      901 gcccttttca cccattgcag gggcaatgtg cgcagcgaag ccgcctggag ggcaaatcac
      961 aaggaagtga tttgtgctcg ggaggcagaa aggggatttt gggcagggca caaggcagct
     1021 gggaaaacag gccaagctgc ctaaccgaat aatatgggga ggagaacggg cgttggccgg
     1081 ggctacagac taggcgccac actaagggtc gatggttaag tcagtcacca ctaattgggt
     1141 gccgccagcg tagctactgg aggtggcttt aaactggctg gggaagccta gcagcgggtc
     1201 aaattccata aaaaactggg tttgggccgg gctgtggctg agggtgtagg cgatgcgggc
     1261 gaatatttgt tcgatgctgg cctgttggta atcttccact cgcagcacct gtacaccgct
     1321 gattacctga ctgattttgc cgtctcgcac ccagatggtc accgggctgt aggcg
//
LOCUS       DAOQGS010000850         1371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_75953_length_1371_cov_4.405015, whole genome shotgun sequence.
ACCESSION   DAOQGS010000850 DAOQGS010000000
VERSION     DAOQGS010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75953_length_1371_cov_4.405015"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>1371)
                     /locus_tag="PKZ52_19390"
     CDS             complement(83..>1371)
                     /locus_tag="PKZ52_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNG61993.1"
                     /translation="AARAQPAWAALSGLERGAILRRWGELMLAHERDLAVILTSEQGK
                     PLAEARAEIAYAASYFYWFAGEAERVYGRVLPVGEGRRHWVEQRPLGIVAAITPWNFP
                     AAMLARKLAAALAAGNAVITKPALETPLSALAMVHLGLQAGLPADLVSVLLSDEPAVI
                     GAWFCQAPSIAKISFTGSTATGKWLLERAGSFIKPCSMELGGNAPFIVFADADIHAAV
                     EGFMACKFRNAGQTCISANRLLLAASIADVFLAELKNALDTLNYGDGMDADIQLGPLI
                     HRAACERLGAKIERAKAQGAKVEFFGRDKRVHAVGAFFAPSLVTGVTPSMQLFSEESF
                     GPVVAVTEFTTVDEALALANQTPYGLAAYVFGQNVTTLNRCQRQLQFGMLGINEARIS
                     HAYAPFGGVKNSGFGREGGLEGIGQYQSQQFVAWGE"
BASE COUNT          357 a          413 c          321 g          280 t
ORIGIN      
        1 ttgccaaatt caacgacgct agggcgagtg aggtaatttt tcagaggttc cctaagcgat
       61 aaataacaac cgggataaat acttattccc cccaagcaac aaattgctgg ctttggtact
      121 ggccaatacc ttctaaaccg ccctcgcggc caaaccctga attttttacg ccaccgaaag
      181 gcgcataagc atggcttatg cgcgcttcat ttattcccag cataccgaat tgcaattggc
      241 gttggcagcg attaagcgtt gttacattct gaccaaaaac ataagcggcc aatccatagg
      301 gggtttgatt cgccagggcg agtgcttcat ccaccgttgt aaactcggtt accgcaacca
      361 ccggcccaaa actttcctcg ctaaataatt gcatgctggg tgtaacgcct gtcaccaagc
      421 tgggcgcaaa aaatgcgccc actgcgtgca cgcgtttatc cctgccgaag aattcaactt
      481 tagcgccctg cgctttggca cgctcaattt tagcgcccag gcgctcgcag gcggcccgat
      541 gaattaacgg ccccagctga atatccgcat ccataccgtc gccataattt aatgtatcca
      601 gggcgttttt aagctcggca agaaatacat cggcaatact tgccgccaat aaaagccgat
      661 tcgcactgat acaggtctgc ccggcattgc gaaatttaca ggccataaat ccctctaccg
      721 cagcatgaat atccgcatct gcaaaaacaa taaacggtgc attaccgcct aattccatac
      781 tgcaaggttt tataaaactg cccgcccgct ccagcaacca tttgcccgtt gccgtggagc
      841 ctgtaaaaga aattttggcg atactggggg cctggcaaaa ccaggcacca atcacagcag
      901 gttcatcgct gagcaagaca ctcaccaaat cggcgggtaa gcctgcctgc agccctaagt
      961 gcaccatcgc caaagcagaa aggggtgttt ctaaagccgg tttggtgatc actgcattgc
     1021 ccgccgccag cgcagcggcg agcttgcgcg cgagcatagc ggcgggaaaa ttccacggcg
     1081 taatagcggc cacaatacct aaagggcgct gctctaccca atggcggcgc ccctcgccaa
     1141 ccggcaacac acggccataa acgcgttcag cctctccggc aaaccagtaa aaataggacg
     1201 cggcataggc aatttccgcc cgcgcttctg cgaggggttt accctgttca gaggttaaga
     1261 tcacggccaa atcccgctcg tgggcaagca ttaattcacc ccaacggcgc aaaatagccc
     1321 cccgctccaa acccgacaac gccgcccagg caggctgtgc acgagcggca c
//
LOCUS       DAOQGS010000851         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_76566_length_1365_cov_3.186260, whole genome shotgun sequence.
ACCESSION   DAOQGS010000851 DAOQGS010000000
VERSION     DAOQGS010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76566_length_1365_cov_3.186260"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="nifN"
                     /locus_tag="PKZ52_19395"
     CDS             <1..1029
                     /gene="nifN"
                     /locus_tag="PKZ52_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HNG61994.1"
                     /translation="HRFPQYAQTAVVAVNTPDFSGSFESGFAVAVKGIVETLVPADTS
                     RVGLRKRQVNVLCGANLTPTDIEFVAESVESFGLRPLLIPDLSGSLDGHLDDDNFNPL
                     TTGGVSVADIATACESIATLAVGESLHPAADALAKRTGVPAHKFGHLLGLEAADEWIN
                     TLAQISGNPVPSRWERHRKQLQDAMLDTHFMIGQARIAIAADPDMLLGFERLLTTMGA
                     EVVAAVVPDRAKSLEASRIKTIQIGDLEDLERRAQAGRAQLMIANSHAAESAERLGVP
                     LLRAGFPQYDLLGGFQRLWFGYRGTTQALFDLANLINAHHQETKPYYSMYAQKLDGPQ
                     GPRAELAH"
     gene            1043..>1365
                     /locus_tag="PKZ52_19400"
     CDS             1043..>1365
                     /locus_tag="PKZ52_19400"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNG61995.1"
                     /translation="MSQITRRLQVLDDKDDSTLLKVAFATSDRQSVDQHFGSAASFAI
                     YGVNMDEARLFNVVEFGALAQDGNEDKLASKLELLEGCIAVYCRACGASALRQLLAIG
                     VQPVKV"
BASE COUNT          270 a          421 c          413 g          261 t
ORIGIN      
        1 caccgcttcc cccaatacgc ccaaaccgct gtggtggcgg tgaacacccc ggatttcagc
       61 ggcagttttg agagcggctt tgcggtggcg gtgaagggca tagtggagac tttagtaccc
      121 gccgacacca gccgagtggg gctgcgcaag cggcaagtta acgtgctctg cggcgccaac
      181 ctcacaccca ccgacataga atttgtggcg gagagtgtag agagtttcgg cctgcggcca
      241 ctgctgattc ccgacctgtc cggctctttg gacggccatt tggatgatga caacttcaac
      301 cccctcacca ccggcggcgt gagtgttgcc gatatagcta ctgcctgcga aagcatcgcc
      361 actctggcgg tgggtgagag cctgcacccg gcggcggatg cgctggccaa acgcactggc
      421 gtacccgccc ataagttcgg ccatctgctc ggccttgagg cggcggatga gtggattaat
      481 accctggcgc agattagcgg caacccggtg cccagccgct gggagcgcca tcgcaagcag
      541 ctgcaggatg ccatgctgga cacccacttt atgatcggcc aggcccgcat cgccatagcc
      601 gccgaccccg acatgttatt gggctttgag cgcctgctta ccaccatggg cgccgaagtg
      661 gtggccgctg tggtgcctga tcgcgccaaa tccctcgagg caagccgcat caaaaccatc
      721 cagatcggcg atttagaaga cctagagcgc cgcgcccagg cgggccgggc ccagctgatg
      781 atcgccaaca gccacgccgc cgagagcgcc gagcgcttag gggtgccctt actgcgggcg
      841 ggcttccccc agtacgacct tttaggcggc tttcaacgcc tgtggtttgg ctatcgcggc
      901 accacgcaag ccttgtttga tttggcgaat ttaatcaacg cccaccacca ggaaaccaag
      961 ccctattact ccatgtacgc ccagaagctc gatggccccc aaggcccccg tgccgagttg
     1021 gcacactgac gtaggagcgc atatgagcca gataacccgt aggttgcagg tacttgatga
     1081 caaggacgac agcacgctgc tgaaggtggc ttttgctacg tcagaccgcc aaagcgtgga
     1141 tcaacacttc ggctcggcgg cttcctttgc catctatggc gtgaatatgg acgaggcgcg
     1201 gctgtttaat gtcgttgaat ttggtgctct ggcccaagac ggcaatgagg acaaactggc
     1261 gagcaagctt gaactgctcg aaggctgcat agccgtgtac tgccgcgcct gtggcgcctc
     1321 ggccctgcgg cagttgctgg ccataggcgt gcagccggtc aaagt
//
LOCUS       DAOQGS010000852         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_77155_length_1359_cov_4.634969, whole genome shotgun sequence.
ACCESSION   DAOQGS010000852 DAOQGS010000000
VERSION     DAOQGS010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77155_length_1359_cov_4.634969"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..535)
                     /locus_tag="PKZ52_19405"
     CDS             complement(191..535)
                     /locus_tag="PKZ52_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61996.1"
                     /translation="MSTPCIIATRLENLSYRLIYCQNDGYDSGNGAGPNLRRFFSDQE
                     RAEVLTALGDISCLWDGKATAYHRDLGESWAHNQPRFADTLSSVRLIAWQTNVAYLYV
                     FEGGHWHRHDIR"
     gene            complement(641..>1359)
                     /locus_tag="PKZ52_19410"
     CDS             complement(641..>1359)
                     /locus_tag="PKZ52_19410"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNG61997.1"
                     /translation="GFFISGILKSPMKTICYVDGYNLYYGCLKNTAFKWLDLHALLTR
                     ILHVQNPTTEIVAIKFFTAPVITKFATHGDLAQASQQQYHRALIALYPDTMQIINGYY
                     TTGKHNAMVYQAPPNKEQRVDVWRLEEKQTDVNLALQAYRDAAMGLVQQLVFVTNDTD
                     QEPTLKQIQEDFADRIKIGVVLPISEPRDESAARPGNRRLSVFADWTRKHIKMDELLQ
                     AQLPSVIPTGKKPIRKPVYW"
BASE COUNT          328 a          333 c          344 g          354 t
ORIGIN      
        1 ttgcattggc ccgcaccgag cgggatgcct ggttgaactg gcctgcccgt gtgtcatcgc
       61 agttggccgc caggctggcc gaggatccac acctggttca tatcgcgctg gaagctctgg
      121 tgcgtgaaca cctacaggag ttgggcgatc tgcgcccgcg cgtggattaa acccgcacct
      181 cgtgtcacgg ctaacgaata tcgtggcgat gccagtgccc gccttcaaac acgtagagat
      241 aggcaacatt ggtttgccag gcgattaatc gcacgctcga cagagtatcg gcgaagcgag
      301 gctggttgtg tgcccagctc tcgcccaaat cccgatggta ggccgtcgcc ttaccgtccc
      361 aaagacatga aatatctccc agtgccgtca acacctcggc acgttcctga tcgctaaaaa
      421 aacgtcgaag gttgggaccg gctccgttac ccgagtcgta accgtcattt tggcaataaa
      481 ttaggcggta actcaggttt tctaagcggg tggcgatgat gcagggggtg ctcataacgt
      541 gggcgggttc aggctgcggt tagacctata aacgctctaa tattcagccg atgcaaccat
      601 tttttgatta ataattatta aaaatcaatt agttaaacca ctaccagtaa actggtttgc
      661 ggatgggttt tttacctgta ggtatgacgc tgggcaattg agcctgtagc agctcatcca
      721 ttttgatgtg ttttcgggtc caatcggcaa aaacacttaa gcgacgattg ccgggccgag
      781 cggctgattc gtcccgaggt tctgaaatag gtaacacaac accgatcttg attcgatccg
      841 caaagtcttc ctgaatctgc ttaagcgtcg gttcttggtc cgtatcattg gtgacaaaaa
      901 ccagttgctg caccagcccc atcgctgcat cgcggtaggc ctgcaatgca aggttaacat
      961 ccgtttgttt ttcttctaaa cgccatacat caacacgctg ctccttgttg ggcggtgctt
     1021 gatacaccat ggcgttatgt ttacccgtgg tgtagtaacc attgataatc tgcatcgtat
     1081 cgggatatag cgctatcaaa gcacgatggt attgctgctg cgaagcctga gctaagtcgc
     1141 catgggtcgc aaatttggtt atgacaggtg ccgtaaagaa tttaatcgca acgatttcag
     1201 tggtcggatt ttgtacgtgc agaatccgtg ttaacagcgc gtgaagatca agccatttaa
     1261 acgcagtgtt tttcaaacat ccgtaataaa gattgtagcc atcaacgtag catattgttt
     1321 tcattggact ttttaatatc ccagatatga aaaaaccac
//
LOCUS       DAOQGS010000853         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_77170_length_1359_cov_3.450153, whole genome shotgun sequence.
ACCESSION   DAOQGS010000853 DAOQGS010000000
VERSION     DAOQGS010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77170_length_1359_cov_3.450153"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..1262
                     /locus_tag="PKZ52_19415"
     CDS             225..1262
                     /locus_tag="PKZ52_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG61998.1"
                     /translation="MASNHLLIKVLTTAVTLALVGCGGGPASSSSSSSSSSSSSSSSS
                     SSSSSSSSSSSSSSSSSSSSSSSSSSSGIPVVRCENPSSHPAPTRVSFTNRGAALSGT
                     YGAVVERESALPRHTVYRPATLPTGTASIPIVSWGNGGCSANGSSHAEFLAEIASHGY
                     IVIANGAPNGSGSDPDNGSTHLAAIDWAIDESTDPCSQYYNRINAKKVAVMGYSCGGL
                     MALNASADPRLSTVVAMNSGLFSATPSVYNGMHTPVAYFNGGTSDIAYENGKRDYQNI
                     TRVPVYFANLPVGHGGTYNDDNGGEFAKVGVAWLNWHLKGDQGTTGKGMFLGTNCGIC
                     RSNWVIESKNF"
BASE COUNT          307 a          432 c          335 g          285 t
ORIGIN      
        1 aaatagccca acccggcagg caatcactga cacccgataa ggacccctta aataaaaagg
       61 atcctctaaa taaagaagct ctgattattg ggtattttca aaacctaggc ctgcctgtat
      121 gcgcagcttt aggtgcctgg ccccccgcga tattgccgaa aatacccgct aggcagaggt
      181 ttctccacat caaaactcac tcactattga acaggtatca aaccatggcc agtaatcacc
      241 tactcataaa agtgttaacc acagctgtga ccctcgctct ggtgggctgt ggaggcggcc
      301 ccgcttctag cagttcatcg tcctcgtcga gttcgagttc cagctccagt tcatccagct
      361 cttcctcatc ctccagctct tcgtctagct cctcatcttc atccagcagc tcatcatcaa
      421 gctccagctc atccagcgga atacctgtgg tgcgctgcga aaaccccagc agccacccag
      481 cccctacacg ggtaagtttc accaatcgcg gtgccgcttt atccggcacc tatggggcag
      541 tggtggagcg agagtccgcc ctgccgcgcc atacggtgta ccggccagcc accttgccca
      601 ccggcacagc ttctattcct attgtctcct ggggtaatgg cggttgctcg gccaatggca
      661 gcagccatgc ggagttctta gcggaaatcg cctcccacgg ctacatcgtc atcgccaacg
      721 gcgcccccaa cggcagcggt agcgacccag ataacggctc cacccatctg gcggccattg
      781 attgggcaat cgacgaaagt accgacccct gcagccagta ctacaaccgc atcaacgcta
      841 aaaaagtcgc cgtgatgggt tattcctgcg gcggcctgat ggcccttaac gcctccgccg
      901 acccgcgcct atccacagtt gtcgccatga acagcgggct ctttagcgca accccaagcg
      961 tctacaacgg tatgcacacc cccgtggctt atttcaacgg cggcacctcg gatatcgcct
     1021 atgaaaatgg caagcgggat taccaaaaca tcacccgcgt acctgtgtac ttcgccaacc
     1081 tgccggtggg ccatggcggc acctataacg atgataacgg aggtgaattt gccaaggtgg
     1141 gcgtggcctg gttgaactgg catctaaagg gcgaccaggg cactacgggc aaaggcatgt
     1201 tcctcggcac caactgcggt atctgtagga gcaactgggt gattgaatct aagaactttt
     1261 aatgcgttaa tcacccgccc ttggcccgca gggctggggc gactcaaccc cgccatgtca
     1321 ccggcggggt tttcttttga ttgcaacagc caggcgcct
//
LOCUS       DAOQGS010000854         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_77716_length_1354_cov_3.566590, whole genome shotgun sequence.
ACCESSION   DAOQGS010000854 DAOQGS010000000
VERSION     DAOQGS010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77716_length_1354_cov_3.566590"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1354)
                     /locus_tag="PKZ52_19420"
     CDS             complement(<1..>1354)
                     /locus_tag="PKZ52_19420"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNG61999.1"
                     /translation="NGGKLVEPIKPPVAKVANINRYDGERDTTVNFDEGWKFNLGDVS
                     GGQNSSFNDASWSAINVPHDWSINLPFNEKSPAAHGGGFLDGGIGWYRKTFSLPASAA
                     GQKLYIQFDGIYMDSTVWLNGQQVCARPYGYTSFECDITAAAKIAGSNLLAVKVINQQ
                     PSSRWYSGSGIYRHVWLKTVNPVAVSFMGTFVKTPSVSANSAAVEININVQNLSAANQ
                     MVTLQNVIFDAQGNQVEDGTIEATAINANSNKDLKQTLTVSNPALWSPDAPALYSVVT
                     SLMVNGTKVGSYTTTFGIRTFELNANTGFWLNGKQLKINGVCLHHDLGALGAAVNTRA
                     IEKRFEMLKQMGTNAIRTSHNPPAPEFLDLADRMGFIVMDEAFDMWYGAKTANDYARF
                     FNDWSERDIKDMVRRDRNHASIIFWSIGNEVPQAADQNAAKRLIGFVRRRIYGLPLPK
                     P"
BASE COUNT          342 a          337 c          308 g          367 t
ORIGIN      
        1 ggctttggca gcggcagtcc gtagatcctt cgacgcacaa aaccaatcag gcgtttggcc
       61 gcattttggt cggcagcctg gggcacttca ttgccaatac tccagaaaat aatactggcg
      121 tgattgcggt ccctgcgcac catatcctta atatcccgct ctgaccaatc gttaaaaaag
      181 cgtgcgtaat cgttagcggt tttagcgccg taccacatat caaaggcctc atccatcaca
      241 ataaacccca ttctatcggc caagtccaaa aattctggcg cagggggatt gtgggaagtg
      301 cgaatagcat tggtgcccat ttgtttgagc atttcaaagc gtttttctat ggcgcgggta
      361 tttaccgccg cgcctaaggc gcctaaatcg tggtgcaaac atacaccgtt aattttaagt
      421 tgtttgccat tgagccaaaa acccgtattg gcatttagct caaaagtgcg gatgccaaat
      481 gtggtggtgt aactacccac ttttgtgcca ttcaccatta ggctggtaac aacggaatac
      541 aacgcaggag catccggcga ccaaagcgca ggattactga cagttaaggt ttgtttaaga
      601 tctttattgc tattggcatt gattgccgtg gcttcgatag tgccgtcttc tacttgattg
      661 ccctgggcgt cgaaaattac attctgcagc gtgaccattt gattggcagc gcttaggttt
      721 tgcacattaa tattaatttc taccgcggca ctattggctg aaacactggg cgttttaaca
      781 aaagtaccca taaaacttac cgccactgga ttaacagttt taagccacac atgacgataa
      841 ataccgctgc cagaatacca gcggctgctg ggttgctgat taattacctt aacggccaat
      901 agattactgc cagcaatttt ggccgctgcg gtaatatcac attcaaacga ggtataacca
      961 tagggccgcg cacacacctg ttggccgttt agccatacgg tgctgtccat ataaatacca
     1021 tcaaattgaa tatacagttt ttgcccagcg gcgctggcgg gcagtgaaaa tgttttgcgg
     1081 taccaaccta tgccgccgtc taaaaatccg ccgccgtggg cggctggaga tttttcatta
     1141 aagggtaaat tgatgctcca atcgtggggc acattaatgg cgctccagct ggcatcatta
     1201 aaactgctat tttgcccgcc gcttacatcg cctaaattaa atttccagcc ctcatcaaaa
     1261 ttcaccgtgg tatcgcgctc accatcgtag cggttgatat tggcaacctt ggccaccggc
     1321 ggttttattg gctctactaa tttaccgccg ttat
//
LOCUS       DAOQGS010000855         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_78291_length_1349_cov_2.708655, whole genome shotgun sequence.
ACCESSION   DAOQGS010000855 DAOQGS010000000
VERSION     DAOQGS010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78291_length_1349_cov_2.708655"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..817
                     /locus_tag="PKZ52_19425"
     CDS             230..817
                     /locus_tag="PKZ52_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNG62000.1"
                     /translation="MTLPFSQSCENNKEPILTVLKEAFSSVRQVLEIGAGTGQHSAFF
                     APALPHLKWQCADLAVNLAGIKQWHEAYPSANLLPSIELDMANPLWPTGFDAVFSANT
                     AHIMPWPLTQKMLVEVATHLPPGGVFALYGPFNYKGDFTSESNERFDRFLKEQAPHMG
                     IRDFEAVNDLACISGLKLHEDKPMPANNRCLIWEK"
     gene            complement(894..>1349)
                     /locus_tag="PKZ52_19430"
     CDS             complement(894..>1349)
                     /locus_tag="PKZ52_19430"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNG62001.1"
                     /translation="QISHYYDVRVALENLVLVEISQLRDLSELQALAERWDPAKLFYG
                     LDITPELKQAEEDFHQELADISRNSVLAGYIADINDHIRVVRLLGFPNEKSVIDTYEE
                     HFRICSYLLQHNLAAAQDEMANHIRKSQDQANRVTLHQIYNNRQILKFD"
BASE COUNT          377 a          301 c          298 g          373 t
ORIGIN      
        1 cgccgtgaac acatccgtgt aggctcgtca aaaaggtcct tctttttgac ggtctgcaat
       61 aacctgtggt caccactgct acactgcgaa acgactatgc ggcatggcta aatactgcag
      121 aataagtcac tcccgcggat aggttaatca ggcgaactga aaggttacag tttaaataga
      181 tacgtacttt aataagcaac atctgtcgat ttaaaaaaga ggatctgcga tgactctgcc
      241 tttttcccaa tcctgcgaaa ataataaaga accgatttta accgtattaa aagaagcatt
      301 ctcatcggtt cgccaggtgc tggaaatcgg cgcaggtaca ggccagcatt cggcattttt
      361 tgctccggca cttccacacc taaaatggca atgcgcagat ttagcagtta atttagccgg
      421 tattaaacaa tggcatgagg cctatcccag cgcgaatctg ctgccgtcta ttgagttgga
      481 tatggcaaat ccgctttggc ctacaggctt tgatgcagta ttttctgcca ataccgcgca
      541 tataatgcct tggccgctga cgcaaaaaat gctggtggag gtggcaaccc atttgccacc
      601 cggcggagtg tttgccctgt atgggccatt taattataag ggggacttta ccagtgaaag
      661 taatgagcgc tttgataggt ttttaaaaga acaagcgccc catatgggca ttcgagattt
      721 tgaagcggta aatgatttag cctgtataag cgggctaaaa ttacacgagg ataaacccat
      781 gcctgctaat aatcgctgtt tgatatggga aaaataaaat ggatgaatca ctaagccctt
      841 taaccaaaaa accgcagtca ccctcgctgt gagttcaact gcggttttta ggtttaatca
      901 aattttaaaa tctgccgatt attataaatt tgatgcaatg tcactcgatt ggcttgatcc
      961 tggcttttac ggatatggtt agccatttca tcctgagctg cggccaggtt gtgttgtaat
     1021 aaataggagc aaatgcgaaa gtgttcttcg taggtatcga taacactttt ttcattggga
     1081 aaccccaata aacgcaccac gcgaatatga tcgttaatat ccgctatata accggctaat
     1141 accgagttgc gggaaatatc cgctaattcc tggtgaaagt cctcttctgc ttgtttgagc
     1201 tccggcgtta tatccaagcc gtaaaataat ttagctgggt cccagcgttc ggcaagcgct
     1261 tgcaattcgc ttaaatcgcg caactgactg atctctacca acaccagatt ttccagtgcc
     1321 acccgcacat cgtaataatg gctgatttg
//
LOCUS       DAOQGS010000856         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_78478_length_1347_cov_3.726006, whole genome shotgun sequence.
ACCESSION   DAOQGS010000856 DAOQGS010000000
VERSION     DAOQGS010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78478_length_1347_cov_3.726006"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKZ52_19435"
     CDS             complement(<1..893)
                     /locus_tag="PKZ52_19435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNG62002.1"
                     /translation="MPYDVVIVGAGPAGLAAACRLKQLSAEISVCVVEKGSEVGAHIL
                     SGAVFEPRALNELFPDWQQLGAPLKTPVSSDAIYYFSQADKGIKVPGFFVPKTLHNEG
                     NYIISLGNLCRWLAGQAEALGVDVFPGFAASELLFTEAGAVKGILIGDMGVGADGEQK
                     SSYTPGMELHGKYTLFAEGCRGHLGKRLIAQFALDAGKEPQHFGLGIKELWQIPAEQH
                     RPGLVVHTAGWPLAESASHGGGFLYHLEDRQVAVGLITDLSYENPHLSPFDEFQRYKH
                     HPTIAQFLKGGKRIAYGARAIA"
     gene            1115..>1347
                     /locus_tag="PKZ52_19440"
     CDS             1115..>1347
                     /locus_tag="PKZ52_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNG62003.1"
                     /translation="MKILVPIKRVVDANVKVRPNDSQSQVDTANLKMAVNPFCEIAIE
                     EAVRLVERGVATEVIAVCIGDTKAQEQLRSALA"
BASE COUNT          315 a          393 c          365 g          274 t
ORIGIN      
        1 gcaatggccc gcgcaccata ggcgatgcgt ttgccgccct ttaaaaactg cgcgatggtg
       61 gggtggtgtt tgtagcgctg gaattcatca aaggggctta agtgggggtt ttcgtaggat
      121 agatcggtga tcaaccccac ggccacctgg cgatcttcca ggtggtataa aaagccgccg
      181 ccatggctgg cggactcggc caatggccaa ccggcggtat gcaccactaa ccctgggcgg
      241 tgttgctcgg cagggatctg ccacagctct ttaatgccca agccaaagtg ctgcggctct
      301 ttgcctgcgt ctaaagcgaa ctgggcaatt aaccgcttgc ccaaatggcc ccggcagccc
      361 tcggcgaaca gggtgtattt gccatgtaat tccatgccgg gggtgtaact gcttttctgc
      421 tcgccatcgg cccccacgcc catatcgccg atgagtatgc ctttaacggc gccggcttcc
      481 gtaaataaca gctcactggc ggcgaagccc gggaacacat caaccccgag agcttcggcc
      541 tgaccggcga gccagcggca caggttgccc aagctaataa tgtagttgcc ctcgttatga
      601 agggttttgg gcacaaaaaa ccccggcact tttatgcctt tatccgcctg gctgaagtaa
      661 taaatggcgt cgctactgac gggggttttt aagggtgccc ccagctgctg ccaatcgggg
      721 aagagttcgt ttagggcgcg aggctcaaat acggcgccgg agagaatatg ggcgcccacc
      781 tcggagccct tttccaccac acatacggaa atctcggcgg ataactgctt taggcggcag
      841 gcggcggcaa gcccagctgg gcccgccccc acaattacca cgtcataagg catagactct
      901 cgttccacag gcacacccca cttggccaaa aagcgcgaag tataaaagca ataggcgcta
      961 aacaatacgc cgccagaggg agaatttgtg taaaaggcta gccacacaaa actaatgctt
     1021 ttagtatcgc cccttttgtt agtctgacac acgccccgct gcccgctggt ttcggcaacg
     1081 gccgctcaag cccaatcagg agagctaaac ccctatgaaa atcctcgtcc ccattaaacg
     1141 ggtggtcgat gccaacgtta aggtgcgccc taacgatagc caaagccaag ttgatacggc
     1201 caacctcaaa atggctgtga accccttctg tgaaatcgct atagaagaag ctgtacgctt
     1261 agttgagcgc ggtgtggcca cagaagtcat cgccgtatgt atcggcgata ccaaagccca
     1321 ggagcaattg cgctccgccc tcgccat
//
LOCUS       DAOQGS010000857         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_79330_length_1339_cov_4.017134, whole genome shotgun sequence.
ACCESSION   DAOQGS010000857 DAOQGS010000000
VERSION     DAOQGS010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79330_length_1339_cov_4.017134"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..849
                     /locus_tag="PKZ52_19445"
     CDS             322..849
                     /locus_tag="PKZ52_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62004.1"
                     /translation="MHFAVFYGHAVKVSKSILTQRIRAADWLIIDAEAEYLLKNAGEL
                     IAESAAVKVLVAEKLMKPNCNFDFCVCPNYDVDGNPGGYIRMAIEEKSEDVSVHFIAE
                     GITLVNTSAPRLNYLKQQIAHTYAKRAQLKAEMEAWYQAGKGLRFPKRLELESIDRYL
                     SQLDTAYKRLWDANQ"
     gene            859..1254
                     /locus_tag="PKZ52_19450"
     CDS             859..1254
                     /locus_tag="PKZ52_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62005.1"
                     /translation="MVFTISAYYPDAELKESEFAAALTRMAIALNMAKSHAAQYLGPE
                     LEVSFMLACDQEAPGFSGMRMGQFDSVEKRLRFETAVPQRFNHSTDAALYIKAALLDA
                     AENAADFLLEQNYLFDVKSHMSLVEGLNP"
BASE COUNT          359 a          276 c          365 g          339 t
ORIGIN      
        1 atttttttgc caaattcaac gacgctaggg cgagtgaggt aatttttcag aggttaccta
       61 gcaaggtagt atttcaacaa gctgttaaag caaggtaagg gtttttcagt tgttgccatg
      121 gctggtggta tctgtttgca gaggtgaaag gtggacgctg agtattgcat cgaaacgccc
      181 aatattggca gccagttgag caattggtgt gctgcggtgg atttttttaa agggaaaatg
      241 gccggtaaaa acgtagtgtt tatagcggac ggagcgtgca accaggccct gcgggtttat
      301 ttatcccagg cgaaattacc catgcatttt gccgtgttct atggccacgc agtaaaggta
      361 agtaaatcaa tattaaccca gaggatacgg gccgctgact ggttaattat cgacgctgag
      421 gcagagtatc tattaaaaaa cgccggtgag ctaatcgcag agagtgcagc tgtaaaggta
      481 ctagtggctg aaaagttgat gaaaccaaac tgcaattttg atttttgtgt ttgcccaaat
      541 tatgacgtcg acggaaatcc tggtgggtac atccgcatgg ctatcgaaga aaagagtgag
      601 gatgtgagtg tgcactttat tgctgagggt ataacgctgg taaataccag tgctccaagg
      661 cttaactatt taaaacagca aatcgcccac acctatgcca aaagggcgca attaaaagca
      721 gaaatggagg cttggtacca agcggggaag gggctgcgtt tccctaagcg cttggagctt
      781 gagtcgattg atcgctacct gtcccaactc gataccgcct ataagcggct ctgggatgcc
      841 aaccaatgag gtgtcaacat ggtctttacc ataagcgcgt attatcccga cgcagaatta
      901 aaagaaagcg agtttgccgc agcgctcacg cgcatggcca tagccctgaa tatggccaaa
      961 tcccacgcag cccaatacct ggggcctgaa ttagaagtga gttttatgct cgcttgtgac
     1021 caggaagcgc cgggtttttc gggcatgcgt atggggcaat ttgatagcgt ggaaaaacgc
     1081 ctgcgctttg aaacagccgt gccgcaacgc tttaatcact ctaccgatgc tgcgctttat
     1141 attaaggctg cgctgctgga tgcggcggaa aatgcggcgg attttttgct ggagcagaat
     1201 tatttgttcg acgtaaaatc ccatatgtcc ttagtggaag ggcttaatcc ttaatagcca
     1261 tggcgcctgt tgcacttagg gaatctctga aaaatgtagg cggcgaagct ctggctaggc
     1321 gaatttggca aaaaaagcg
//
LOCUS       DAOQGS010000858         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_79349_length_1339_cov_3.302181, whole genome shotgun sequence.
ACCESSION   DAOQGS010000858 DAOQGS010000000
VERSION     DAOQGS010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79349_length_1339_cov_3.302181"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKZ52_19455"
     CDS             <1..254
                     /locus_tag="PKZ52_19455"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNG62006.1"
                     /translation="AVLIQAHIARAAMLVVSTPDTFDVRQMIKNARTLNPKIETLVRT
                     HNETEAEFLEQEMAQKVFLGENELAKSMIGYILERFGKV"
     gene            complement(260..>1339)
                     /locus_tag="PKZ52_19460"
     CDS             complement(260..>1339)
                     /locus_tag="PKZ52_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62007.1"
                     /translation="IEPASTGDEAKNKDRVFKRATSDLISKVMLVTHITKSVTYEGHH
                     YSQYLYNAYEQDLDGTYNFAESFAKVDLMFADMATQLKQAREAGAPFTHVIVMSMGWN
                     NDQVESLYRYNTIIKNVQQEALANKQEFKPLLICFTWPSVWGGVTDSVIFRTAKHLAS
                     YVNKTDDADENGYTWGNWVVNKKIPEALAAADLSGAKPKLVLIGHSFGARFLSRALFS
                     ARFINAEYATHNTVDVFLGLQGAFSLRRFVADAGVEGSPYAGFASLPGKIVLTSSKHD
                     TSNLAALWAANAGGPQGLNYARKTPAVFSVLDWSSSGGALPMAANKITLVDASSIVKH
                     ESDKIDAHNDILDADMARLIWAAIH"
BASE COUNT          377 a          342 c          290 g          330 t
ORIGIN      
        1 ccgccgtatt aattcaagcc cacatagccc gtgcagccat gctggtggtg agcacccccg
       61 acacctttga tgtgcgccaa atgattaaaa acgcccgcac cctcaacccc aaaatagaaa
      121 ccctggtgcg cacccacaac gaaacggaag cggaattttt agaacaggaa atggcccaga
      181 aggtatttct gggcgaaaac gaattggcga aaagtatgat tggctatatt ttagagcggt
      241 ttggcaaggt ttaaatttat tagtgaatag ccgcccaaat taacctggcc atgtcggcat
      301 ctaaaatatc gttgtgggca tcaattttat ctgattcgtg cttaacaatt gaggatgcat
      361 caaccagcgt tattttattc gccgccattg gtaatgcgcc gccactgctg ctccaatcca
      421 aaacactaaa aaccgctggg gttttgcgcg cataatttaa accctgcggg ccgcccgcat
      481 tggcagccca aagtgccgcc aggttagagg tatcatgctt ggaactggtt aaaacaattt
      541 tgccgggcaa agatgcaaac cctgcataag ggctgccctc aactccggca tccgcaacaa
      601 accggcgcag gctaaacgcc ccctgcaagc ctaaaaacac atcaaccgta ttgtgggtgg
      661 cgtactcagc attaataaaa cgcgcagaaa ataaagcgcg acttaaaaag cgcgcaccaa
      721 agctgtggcc aattaatacc agtttcggct tagcgccgga taaatctgct gccgcgagcg
      781 cttcggggat ttttttattc accacccaat tgccccaggt ataaccattt tcgtcggcat
      841 catcggtttt attcacataa ctggccaggt gtttagcagt tctaaaaatc accgaatccg
      901 taacaccgcc ccacaccgaa ggccaggtaa aacaaataag caagggttta aactcttgtt
      961 tattggcaag agcttcctgt tgaacatttt taataatggt gttgtagcgg tacagggact
     1021 ccacctgatc attattccac cccatggaca tcacaatcac gtgggtaaaa ggcgctccag
     1081 cttctcgggc ttgttttagc tgcgttgcca tatcagcaaa cataagatca actttggcaa
     1141 acgactcggc aaaattatag gtaccgtcca aatcctgctc ataggcgtta tacaaatact
     1201 gcgaataatg atgcccctcg taggttacgc ttttggtaat gtgggttacc aacatcacct
     1261 tactgattaa atcagaggta gcccgtttaa atacccggtc tttatttttt gcttcatcgc
     1321 cagtagaagc cggctcaat
//
LOCUS       DAOQGS010000859         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_79564_length_1337_cov_3.353354, whole genome shotgun sequence.
ACCESSION   DAOQGS010000859 DAOQGS010000000
VERSION     DAOQGS010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79564_length_1337_cov_3.353354"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..>1337)
                     /locus_tag="PKZ52_19465"
     CDS             complement(189..>1337)
                     /locus_tag="PKZ52_19465"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HNG62008.1"
                     /translation="DAEEFVLWIYFGYTANAELKELARQIFEQAPIPILQVTFARKDD
                     WNLSKLEAGSLTDLTEDQKTDFANALDSYSHLIWRKARAKKNYRYDMAILVDAEEPFP
                     PSNKLALKKFISAAKKHDIRAETITRKDLGKLAEYDALFIRATTSVNHYTYKFARLAQ
                     AEGLVVMDDPDSILRCTNKIYLAQLLETHGLPIPKTRVLTRVDMTRIDDLIADLGLPI
                     VLKVPDGSFSVGVKKAKSKEELEADLKSMFKKSALLIAQAYLYTEFDWRIGMLNGAPL
                     YACRYYMAKNHWQIYNHSGPSTTKSGGFDAMPTFEVPKQVIRAAVNACKQIGNGLYGV
                     DIKQSGKEVYIIEVNDNPSIDSGVEDQYLEGDLYDEIMEEFRRRLDDQ"
BASE COUNT          363 a          324 c          279 g          371 t
ORIGIN      
        1 attcacccta aggtgccaag tgcttgttta cctgccactc taaacactta ggcgtttaga
       61 actggcggtg cgcatttcac taaataaccc aaaggtgcaa actacgcgct ctgggtaaat
      121 ttaccttgca gagcttcgcc acctacattt tcacaacgcc cctgaatttt gataggcata
      181 gaatttaatt attggtcatc caagcggcgg cgaaattctt ccataatttc atcatacaga
      241 tctccttcca aatattgatc ctccacgccc gaatcaatcg acgggttgtc attcacctca
      301 ataatataaa cctccttgcc ggattgtttt atatcgacac cataaagccc attaccaatt
      361 tgtttacagg catttactgc agcgcggatc acttgcttgg gcacttcaaa ggtgggcata
      421 gcgtcaaaac cgccagattt tgtggtacta gggcctgagt ggttataaat ttgccagtga
      481 tttttcgcca tgtaataacg gcaggcatat aaaggtgcac catttaacat gccgatgcgc
      541 cagtcaaatt cggtatacaa ataagcctgc gcaattaaca gggcgctctt tttaaacatg
      601 gattttaaat ccgcttcaag ctcttcctta gatttggcct ttttcacacc gacggaaaat
      661 gacccatcgg gcactttaag cacaataggt aggcctaaat cggcaattaa atcatcgatg
      721 cgggtcatat ccacgcgggt taacacacgg gttttaggga tcggcaggcc atgggtttcc
      781 aacaattgcg ccagataaat tttattagta cagcgcaaaa tagaatctgg atcgtccatc
      841 accaccaagc cttcggcctg ggccaagcgc gcaaatttat aggtgtagtg attcactgaa
      901 gtagtagcgc gaataaacag ggcgtcgtat tcagcgagct taccaagatc cttgcgggtg
      961 atggtttcag cgcgaatatc gtgttttttc gccgcgctaa taaatttttt caaagccagt
     1021 ttattactgg gcggaaaggg ctcctcagcg tccactaaaa tcgccatatc gtagcggtag
     1081 tttttcttcg cgcgggcttt gcgccagatt aaatggctgt agctatctaa ggcattggca
     1141 aaatccgttt tttgatcctc cgttaaatca gtcaacgagc ccgcctcaag cttacttaaa
     1201 ttccagtcat ctttgcgggc aaaggttact tgtaaaatag gaataggcgc ctgttcaaaa
     1261 atctgccggg ccagctcttt taactctgca ttggcggtat aaccaaaata aatccacaat
     1321 acaaactcct ctgcatc
//
LOCUS       DAOQGS010000860         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_79588_length_1337_cov_2.756630, whole genome shotgun sequence.
ACCESSION   DAOQGS010000860 DAOQGS010000000
VERSION     DAOQGS010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79588_length_1337_cov_2.756630"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..>1337
                     /locus_tag="PKZ52_19470"
     CDS             378..>1337
                     /locus_tag="PKZ52_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62009.1"
                     /translation="MNSLYQVKAPKFRSRKAFIAWAERVHKMKATYDFDRDNAFIQKY
                     LNSQALDISLLTDSNYIWSTRALHEYLIITAALFEGDTTGLLKLRDTVFNHGITLQIY
                     HVGWELEKVFLENKYPEKPYKPLAGPDIKEFGNYVCLIAIFGNQYYFTQMVKVILDHL
                     DLGHNVVGPYAHVTQQFIMRLAEHFLQLPRRNWEADEGNWAFDPLKREPLFQALWDHW
                     ADEDTAILEPLLMQLLNWHTYQASRQNKDGGQDYYPNLEQIPLAYFFILRLRQWRGLS
                     VPKISHVLTQPPFDCLPDEVEGRYEFCPFAKEFYAKTISLMPDF"
BASE COUNT          361 a          295 c          316 g          365 t
ORIGIN      
        1 tattaattgt gggcctggtg gcgagcttgc atgataagtt gaagtggttt ggcgagtagg
       61 ttggaagagt gaagcgactt ccgacgaaaa gatatttcgt ccaggtgtta attaggctca
      121 atagcagaga ggtgaatgca tggctgaatg cgcggcatat tgcagacttg ggcgcccatt
      181 ggatagctaa atatttatcc tgtcgcgcaa ttaaataaaa tctcgcctag gatttttcga
      241 tagaaatcaa attaaaaata agcacggtgc agttttaatt cgctgtaacc tgctgcgcaa
      301 agggcagaaa attgaggtgc ctcgcccagg tgaagacacc gtcgctcagg atctgacact
      361 aaaagcgtgg aatcaacatg aactcacttt atcaagtcaa agcgcccaaa ttccgctccc
      421 gtaaagcatt tatcgcctgg gcggaacggg ttcacaaaat gaaggccact tatgattttg
      481 atcgtgacaa cgcattcatt caaaagtatc tcaatagcca ggcgctcgat atctcgttac
      541 tgactgacag taactatatt tggagcacta gagctctgca cgaatacctt attattactg
      601 ccgctctttt tgaaggcgac acaacggggc tgttaaaact tcgcgatact gtttttaacc
      661 acggtataac acttcagatt taccacgtcg gctgggagtt ggagaaagtg tttctggaga
      721 ataagtatcc agaaaaaccc tacaagccac ttgccggccc ggatattaag gagtttggca
      781 actatgtatg cctgatcgct atttttggca atcagtatta ttttactcag atggtaaaag
      841 ttattttgga ccacctggat cttgggcaca acgtagtggg cccgtatgct catgtgaccc
      901 aacaatttat tatgcggctg gcggagcatt ttttacagct gccccgccgt aattgggagg
      961 ccgacgaggg taactgggca tttgacccac ttaaaagaga gccattattt caagcactat
     1021 gggatcattg ggccgatgaa gatacggcga tattggaacc cttgttgatg caactgctta
     1081 attggcacac ctatcaagct agccgccaaa ataaagacgg tgggcaggat tattatccta
     1141 accttgagca aatccccctg gcctactttt ttattttgcg tttgcgccag tggcgaggtt
     1201 taagtgttcc taaaatttcc catgtgctta cccagccgcc ttttgactgc cttcctgatg
     1261 aagtggaagg ccgctatgag ttttgcccct tcgctaaaga attttacgcc aaaaccatca
     1321 gcctgatgcc tgacttc
//
LOCUS       DAOQGS010000861         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_79762_length_1335_cov_3.994531, whole genome shotgun sequence.
ACCESSION   DAOQGS010000861 DAOQGS010000000
VERSION     DAOQGS010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79762_length_1335_cov_3.994531"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKZ52_19475"
     CDS             <1..416
                     /locus_tag="PKZ52_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62010.1"
                     /translation="QLQHLHLMADSSLPLGAYRIEAGPSQVVFDAAERLRRWLDEVQP
                     KADLDPALKSYAPPQALASAQEAPAREQETPAGEQETSARERQASASEEQALARAREE
                     PAMQEVSLGSSERDRDSEAEPSAELAQADSEGSAV"
     gene            413..>1335
                     /locus_tag="PKZ52_19480"
     CDS             413..>1335
                     /locus_tag="PKZ52_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNG62011.1"
                     /translation="MSAPAPLTLKPYFHAGDLAAPPVAMGTLTRLVGLTLEALGLNVS
                     VGSHCQVLLPKGRLLDAEVVGFAEDKTYLMPVQKVEGLQPGLKVRPSGTAAAIPMGKS
                     CLGRIINGIGQPIDGKGPLIAEDQLSVHAAAINPLHRDPISQPLDVGIRAINALLTVG
                     RGQRIGLFAGSGVGKSVLMGMMTKFTEADVVVVGLIGERGREVKEFIDHILGEEGLSR
                     AVVVASPADDAPLMRLRAAELSTRVAEYFRDQGLNVLLLMDSLTRYAQAQREIALAIG
                     EPPATKGYPPSVFAKIPDLVERAGNGLAGSGS"
BASE COUNT          266 a          403 c          427 g          239 t
ORIGIN      
        1 cccagttgca gcatttgcac ctgatggcgg acagcagttt gcccctgggc gcctatcgca
       61 tagaagccgg gccgtcccag gtggtgtttg atgcggctga gcggctgcgc cgctggctgg
      121 atgaggtgca gcccaaagcc gacctggatc cggcccttaa atcctatgct ccgccccagg
      181 cactagccag cgcgcaagag gcaccagcca gggagcaaga gacaccagcc ggagagcaag
      241 agacatcagc cagggagcga caggcgtcag ccagcgagga gcaggcctta gccagagcga
      301 gggaggagcc cgctatgcaa gaagtgtctt taggctccag tgagcgcgat cgcgattccg
      361 aggcggaacc atcggccgaa ctggcccagg ccgattcaga aggttctgca gtatgagcgc
      421 cccggcgcca ttaactctaa agccctattt tcacgccggg gacttagcgg ccccacccgt
      481 cgccatgggc accctgaccc gtttggtggg cctgaccctt gaggccctag ggctgaacgt
      541 gagtgtgggc agccactgcc aggtgctctt gcccaagggc cgcctgctgg atgccgaagt
      601 ggtgggcttc gccgaggaca aaacctacct gatgccggtg caaaaagtag agggtttaca
      661 acctggccta aaagtgcgcc ccagcggcac tgcagcggca atccccatgg gcaaaagctg
      721 cttgggccgc atcatcaacg gcataggcca acccatcgac ggcaaaggcc cactgattgc
      781 ggaggatcaa ctcagcgtgc acgctgcggc tattaatcca ttgcaccggg acccgataag
      841 ccagccttta gacgtgggca ttcgcgccat caacgcgtta ttaaccgtcg gccgcggcca
      901 gcgtatcggc ttgttcgcag gctccggcgt gggcaaaagc gtactcatgg gcatgatgac
      961 taaattcacc gaggccgatg tggtggtggt gggtttgatc ggcgagcggg gccgggaggt
     1021 aaaagaattt atcgatcata tcctcggcga ggagggctta tcccgcgctg tggtggtggc
     1081 gtcccctgcc gatgatgcgc ctctgatgcg gctgcgggcg gcggagctat ccacgcgagt
     1141 ggccgagtat ttccgcgatc agggcctgaa tgtgctgctg ctgatggatt ccctcacccg
     1201 ttatgctcag gcccaacggg aaattgccct cgccattggc gagccaccgg caactaaagg
     1261 ctacccgccc tcggtgttcg ccaaaatccc cgacctggtg gagcgcgctg gcaatggctt
     1321 ggccggttcc ggttc
//
LOCUS       DAOQGS010000862         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_80200_length_1331_cov_4.450627, whole genome shotgun sequence.
ACCESSION   DAOQGS010000862 DAOQGS010000000
VERSION     DAOQGS010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80200_length_1331_cov_4.450627"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..516
                     /locus_tag="PKZ52_19485"
     CDS             85..516
                     /locus_tag="PKZ52_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG62012.1"
                     /translation="MNNAINALSVLLIDDDDVALEAVQRSLRKHNVLCPVYTACDGLE
                     GLDILRETHPSKKIPKPYLVLLDLNMPRMNGFEFLNELRADPLLKETVVFVLTTSNND
                     ADRNKAYNENIAGYMVKSAVGPQFQKLALLLEHFSQAVVMP"
     gene            721..>1331
                     /locus_tag="PKZ52_19490"
     CDS             721..>1331
                     /locus_tag="PKZ52_19490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG62013.1"
                     /translation="MLARRFLVVDDDDVDREKVKRLLKIYPAELQVDEAASVGEALIR
                     LKHSVFDCLLIDYRLSDALGTEILSGLRASNRRPLPVIVISGNNSQELVKEVMRAGAQ
                     DCLDKGSLTPEVLCSAIEQALEDINVQCINWDTHTRNRQLAEGLPQLAWSCLPDGTCE
                     YLNRRWCEYTGISQDEQLGFAWLNQVHPDDRDRLVQAWMTSVR"
BASE COUNT          331 a          290 c          368 g          342 t
ORIGIN      
        1 acgaaagcag ggcacggaat ccggcggcaa tcgcggggtg ttgtttcgca ctctggtggc
       61 cccgctttgc gcggagtgat ttagatgaat aatgcaatca atgcactgtc agtgttgtta
      121 attgacgatg acgatgtggc cttagaagcg gtgcagcgca gtttgcgcaa acataatgtg
      181 ttgtgcccgg tgtataccgc ctgcgacggt ttggagggtt tggacatttt gcgcgaaacc
      241 cacccgagca aaaaaatccc caaaccctat ttggtgctgc tggatttaaa tatgccgcgc
      301 atgaatggct ttgaattttt aaatgaattg cgcgctgacc cgctgctaaa agaaaccgtg
      361 gtatttgtat taaccacctc caataacgat gccgaccgca ataaagccta taacgagaat
      421 attgccggtt atatggtgaa gtcggcggtg gggccgcagt ttcaaaaatt ggcactgctg
      481 ctggagcact tttcccaagc cgtggtgatg ccttgagtaa agcacagcac cttgggaaaa
      541 accctaaaaa gctagctaat tttgatttgt ggtaactatg ggcagggctg atcaagcctg
      601 aattcgcgat ttatgagccg tgtgtgcaag ggcttttttt ccacgacggt tttgaaaacg
      661 cctattaggc tgaggctgct gaacattatt ttataagccc cgttttatgg cgagagcatt
      721 atgttagcac gacgtttttt agtggtagat gacgatgatg tagatcgcga aaaggttaaa
      781 cgcctgttaa aaatataccc ggcggaactg caagtggatg aagcagcaag tgtcggggag
      841 gcgttaatta ggcttaaaca ttccgttttt gattgtttgt taattgatta ccgtttaagt
      901 gatgcactgg gcacggaaat attatccggc ctgcgtgcca gcaatcgccg cccgttgccc
      961 gttattgtca ttagcggtaa taacagccaa gagctagtaa aagaagtcat gcgcgcaggc
     1021 gcccaggact gtttggataa gggctctctc accccagagg tattatgcag cgccattgag
     1081 caggcgctgg aggatatcaa tgtgcagtgt attaattggg atacccatac ccgcaaccgc
     1141 caattggccg aaggcctgcc gcaattggcc tggagctgcc tgcccgacgg cacttgtgaa
     1201 tatttgaatc ggcgttggtg tgaatacaca ggcatcagcc aggacgagca attgggtttt
     1261 gcctggttaa atcaggtgca cccggatgac cgagatcgtt tggtgcaggc ctggatgacc
     1321 tctgtgcgca g
//
LOCUS       DAOQGS010000863         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_80682_length_1327_cov_3.481132, whole genome shotgun sequence.
ACCESSION   DAOQGS010000863 DAOQGS010000000
VERSION     DAOQGS010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80682_length_1327_cov_3.481132"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKZ52_19495"
     CDS             complement(<1..186)
                     /locus_tag="PKZ52_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62014.1"
                     /translation="MANTHKLCWHYEPSHFFDDPSECSWQNREFEIADGIVTISVGND
                     QNILNDALRVEIDTELSN"
     gene            complement(228..>1327)
                     /locus_tag="PKZ52_19500"
     CDS             complement(228..>1327)
                     /locus_tag="PKZ52_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG62015.1"
                     /translation="RVRYLPSGPPVPYPVQFRISGPDPQQVRGIADQVKELVRANPNC
                     IGVNDNWNEAIKVLRLDLDQDKLRAVGLTSELVMQAANTILSGATIGQYRENNRLIDI
                     QIRQPLDERDTIERLNDTNIPTQNGTYVTLSNIARAYFVWEPGLIWRENREWAVTVQA
                     DVKDGIQGPTVSSQIIPLLADIKAQLPLGYAVKLAGAAADSSKAQDSIAANVPLVIFI
                     IFTLLMLQLHSFSRAVLVFLTGPLGVAGAALALLILHRPFGFVAQLGVIALFGMIIRN
                     SVILIDQIEQDIARGEPAWNAVVESAVRRLRPILLTAAAAVLAMIPLSRSIFWGPMAV
                     AIMGGLIIATGLTLLFLPALYALCFKVNRHK"
BASE COUNT          357 a          313 c          325 g          332 t
ORIGIN      
        1 attgcttaat tctgtatcta tttccactcg caaggcgtca ttcagaatat tctgatcgtt
       61 gcccacacta attgttacaa ttccatcagc aatctcaaac tctcgatttt gccaactaca
      121 ttcagatgga tcgtcaaaaa aatgacttgg ctcatagtgc cagcatagtt tatgtgtgtt
      181 agccattttt aagtgatctc aaagtaagct gataaatgtt ttatgcttta tttgtgccta
      241 tttactttaa agcaaagcgc gtacagcgcc ggtaaaaata acaatgtaag ccccgtagca
      301 ataattaacc cacccataat ggctaccgcc ataggccccc aaaatatcga gcgacttaag
      361 gggatcatgg cgagtactgc agcggcagcg gtgagcagaa taggccttaa acggcgcacg
      421 gcggattcca cgactgcatt ccaggcgggt tcgccgcggg caatatcctg ctctatttgg
      481 tcaattaaaa taaccgagtt gcgaataatc atgccgaata aggcaattac ccctaattgc
      541 gccacaaaac caaaggggcg atgcaaaatt aacaacgcca gggcggcacc tgccacaccc
      601 aaggggccgg ttaaaaatac cagcaccgcg cgggaaaaac tgtgtaattg cagcattagt
      661 agggtgaaaa taataaaaat gaccaggggt acattagcgg cgatggagtc ttgagctttg
      721 ctgctatcgg cggcggcgcc agcaagtttt acggcgtaac ctaagggtaa ttgcgcttta
      781 atatcggcaa gcagtggaat aatttggctg gaaactgtgg ggccttgaat gccgtcttta
      841 acatccgctt gtacggttac ggcccattcg cggttttccc gccaaataag ccctggctcc
      901 caaacaaaat acgcccgcgc aatattggag agggtgacat aagtgccgtt ttgggtggga
      961 atattggtgt cgttgaggcg ctcgatagtg tcgcgctcat ccaagggttg gcggatttga
     1021 atatcgatta aacggttatt ttctcggtat tggccgatgg tggcgccact taaaatggta
     1081 ttggccgcct gcatcaccag ttcgctggtt agcccaaccg cgcgcaattt atcctggtct
     1141 aaatccaagc gtaaaacttt aatggcttca ttccagttat cgttaacgcc gatacaatta
     1201 ggattagcgc gcaccaattc ttttacctga tcggcaatgc cgcgcacttg ctgagggtct
     1261 ggcccactaa tacgaaattg cactgggtag ggcacaggtg ggccggaggg taaataacgc
     1321 acgcggc
//
LOCUS       DAOQGS010000864         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_81006_length_1324_cov_4.834515, whole genome shotgun sequence.
ACCESSION   DAOQGS010000864 DAOQGS010000000
VERSION     DAOQGS010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81006_length_1324_cov_4.834515"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKZ52_19505"
     CDS             <1..561
                     /locus_tag="PKZ52_19505"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG62016.1"
                     /translation="LTAALDGVVLERALNLGEQTDDKTVFVIADLSSLWVEIALFPSD
                     LAKIHTGQQVRIINPNNAQQAVGELTYLAPQANSVNQAVSGRVVVPNTRHQWRPGQFV
                     QAEVLLAEHPAAIAVCNEALHEHEGKQVVFVQEADGFKPRPVRIGRSDNTWTEIIAGL
                     NTGETYASKNSFILKAELGKDGAEHD"
     gene            554..>1324
                     /locus_tag="PKZ52_19510"
     CDS             554..>1324
                     /locus_tag="PKZ52_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNG62017.1"
                     /translation="MISALLKFSLARRWIIIVLTLLVAALGVWNYQHLPIDAVPDITN
                     VQVQINTEAAGFTPLEVEQRITYLIEVAIAGLPSVEETRSLSRYALSQVTVVFKDGTD
                     IYFARTLINERLQQVKNQLPAGLEPSLGPVATGLGEIFHYTVRADSSARQTNGEPFDA
                     TALRSLQDWVIRPQLRLVPGVTEVNTIGGFEQQFHITPWPAKLLAYGISFEEIITALN
                     NNNANVGAGFIEKNGEQYLIRAPGQIKDMQSLAQIIVAT"
BASE COUNT          335 a          307 c          356 g          326 t
ORIGIN      
        1 ctcaccgccg cactcgatgg tgtggtgtta gagcgcgcac ttaatttagg cgagcaaacc
       61 gacgataaaa ccgtatttgt gattgcggat ttatccagcc tttgggttga aatagcatta
      121 tttccctctg accttgccaa aattcacaca ggccagcagg tgcgcattat taatcctaat
      181 aatgcccagc aggcagtggg tgaactcacc tatcttgccc cgcaggccaa tagcgtaaac
      241 caagcggtga gtggacgggt tgtggtgccg aatacccggc accagtggcg gccagggcaa
      301 tttgtgcagg cggaggtttt attggcggag cacccagccg ctattgcggt gtgcaatgaa
      361 gcgcttcacg agcatgaagg caaacaggtt gtatttgtgc aagaagcaga cggctttaaa
      421 ccgcgcccgg tgcgcattgg ccgcagcgat aatacatgga cggaaataat cgccggttta
      481 aatactggcg aaacctacgc cagcaaaaat agttttattc tcaaggcgga attgggcaag
      541 gatggggctg aacatgatta gcgcactgct gaaattctcc ctggctcgcc gctggatcat
      601 tattgtttta acactgctag tggctgcctt gggggtttgg aattaccaac atttacctat
      661 agatgctgtg ccggatatca ccaatgtgca ggtgcaaatt aataccgagg cagctggttt
      721 tacaccttta gaggtggagc agcgcatcac ctatttaatt gaggtggcga ttgcaggctt
      781 gccctcggtg gaggaaaccc gctctttatc ccgttatgcg ctctcacaag taaccgtggt
      841 ttttaaagat ggtacggata tttatttcgc ccgcacttta attaacgagc ggctgcaaca
      901 ggtaaaaaac caattgcccg cagggcttga gccgagcctg gggccggtgg ccacaggctt
      961 gggggaaata tttcattata cggtgcgcgc cgatagcagc gcgcgccaaa ccaacggtga
     1021 gccttttgat gcaactgcgc tgcgcagcct gcaagattgg gtgatacgcc cgcaattgcg
     1081 tttggtgccc ggtgtgacgg aagtaaacac tattggtggt tttgaacaac aattccacat
     1141 cactccctgg cctgccaaat tattggccta tggtattagc tttgaggaaa taattaccgc
     1201 actaaataat aacaatgcga atgtgggagc agggtttatt gaaaaaaatg gtgagcaata
     1261 tttaattcgt gcacccgggc aaattaagga tatgcagtcg cttgcacaga ttattgtggc
     1321 cacc
//
LOCUS       DAOQGS010000865         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_82007_length_1315_cov_3.531746, whole genome shotgun sequence.
ACCESSION   DAOQGS010000865 DAOQGS010000000
VERSION     DAOQGS010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82007_length_1315_cov_3.531746"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKZ52_19515"
     CDS             <1..471
                     /locus_tag="PKZ52_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018276066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNG62018.1"
                     /translation="PAGVTKAIAELQQVADLLRQRYPEVPLYLDLSELRGYHYHTGIV
                     FAAFAPGLGREIASGGRYDHIGEAFGRARPATGFTLDLIALKRLGAVGDASPSGIFVP
                     FALVSESWQFIQSLRAAGERVVCATASFPDYLPHLACDRKLVQAAGTPAVQPIN"
     gene            493..>1315
                     /locus_tag="PKZ52_19520"
     CDS             493..>1315
                     /locus_tag="PKZ52_19520"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNG62019.1"
                     /translation="MGKNVVILGTQWGDEGKGKIVDLLTEQVKYVARFQGGHNAGHTL
                     VIEGKKTVLHLIPSGILHAGVTCLIGNGVVLSPEALMKEIGELEAQGVPVRDRLRLSP
                     ACPLILPYHVALDLAREHARGNDKIGTTGRGIGPAYEDKVARRGLRLGDLLDPRAFAQ
                     KLRAIVDYHNFALTQYYRVEAVDYDAVLAQALALGDQIKPMICDITEMLHAAREAGEN
                     IMFEGAQGSLLDIDHGTYPFVTSSNTTAGGTATGSGFGPLYLDYVLGITKAYTTRV"
BASE COUNT          273 a          372 c          358 g          312 t
ORIGIN      
        1 ccagctgggg tcacaaaagc cattgcagaa ttacaacagg tagccgatct gctgcgccag
       61 cgttatcccg aggtgccact gtatttggat ctcagcgaat tgcgcggtta ccactaccat
      121 acaggcattg tatttgcggc ctttgcgcca gggcttgggc gcgagatcgc ctccggcggc
      181 cgttacgacc atataggtga ggcctttggc cgcgcccgcc ctgctaccgg cttcaccctc
      241 gaccttatcg cgctaaaacg tctcggcgcc gttggcgatg cctccccgtc tggcatattt
      301 gtcccctttg cgttggtgag cgagtcttgg cagttcattc aatccctgcg ggccgctggc
      361 gagcgagtgg tatgtgcaac cgcaagtttc ccggattatc tgccccacct cgcctgcgat
      421 cgtaagctgg tgcaagccgc tggcaccccc gcagtgcaac ctattaatta acgtacagaa
      481 agagtttttc acatgggtaa gaacgtcgtc attttgggca cccaatgggg tgatgaaggc
      541 aagggtaaaa ttgttgattt gctcaccgag caggttaaat acgttgcccg atttcaaggt
      601 ggccacaacg ctggccacac cctggtgatt gaaggcaaaa aaaccgtttt gcaccttatt
      661 ccttctggaa ttttgcacgc gggcgtaacc tgcctaatcg gcaatggcgt ggtgctaagc
      721 cccgaagccc tcatgaagga aattggtgag ctagaagctc agggggtgcc tgttcgcgat
      781 cgtctgcgtc tatcacccgc gtgcccttta attcttcctt accatgtggc gctagattta
      841 gcccgtgagc acgcacgcgg caatgataaa attggcacca ccgggcgcgg cattggcccc
      901 gcctatgaag ataaagtggc gcgccgcggc ctgcgtttgg gggatttatt agatccgcgc
      961 gcgtttgccc aaaagttgcg cgccatagtc gattaccaca atttcgccct cactcaatac
     1021 taccgagttg aggccgttga ttacgacgcg gtattggctc aggccctcgc gctgggcgac
     1081 caaatcaaac ctatgatttg tgacattacg gagatgctcc acgccgctcg cgaggcgggc
     1141 gaaaacatta tgtttgaagg tgcccaaggc agtctgctgg atatagatca cggcacctat
     1201 ccgtttgtaa cctcttctaa caccactgcg ggaggcaccg ccacgggttc aggctttggc
     1261 cctttgtacc tggattatgt gcttggaatt accaaggcct acaccactcg agtgg
//
LOCUS       DAOQGS010000866         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_82032_length_1315_cov_2.830159, whole genome shotgun sequence.
ACCESSION   DAOQGS010000866 DAOQGS010000000
VERSION     DAOQGS010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82032_length_1315_cov_2.830159"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..>1315)
                     /locus_tag="PKZ52_19525"
     CDS             complement(316..>1315)
                     /locus_tag="PKZ52_19525"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNG62020.1"
                     /translation="SQAWAPYDIPACIERFLTSAKPCGLVIMETELWPNWLHVCRRKN
                     IPSLLANGRLSAKSARGYQRFAALSCQMMNDISMLTVQTEAHAERFHSLGAKRNKIQV
                     VGNIKFDIHLDSAIASKAQAARSILYSRPTFIAGSTHAGEDEIILAAFKQVISRIPNA
                     LLILVPRHPERFDAVAALIQAQGFTLARRSQDHLPKPHQQVLLGDTLGELTLLYGLAA
                     AAFIGGSLIPRGGHNMLEAALWGIPIATGPHMDNFLQLHEDLSQCGALKTLQSANELA
                     EFFQLAYTNPANAKQMGEAGLNYLLSNQGALGRIKGAIDVTLQPLFTGAGRGARTN"
BASE COUNT          320 a          305 c          347 g          343 t
ORIGIN      
        1 cggcaatgtc ggttgcaatg cggaagactt cgaatcacgc gccatgggta acctttttta
       61 ttgtaatgaa gagtgtttgt ctgctggcgt ctttggcgcc ggtaaaaaat attgctgttc
      121 ggtggcgtgt gttttgttaa taagcctccg ggggatttag ttcttgttct atctggtggg
      181 ggctttttta tgctggatca tttgattggc aggtaactat ttagcacgct ttattacgct
      241 cgtcacgcca ccggcaaatt gagaaggatt tacccctgcg ggggctcgca gccctacaaa
      301 cgcgcctggt actttctagt tggtacgagc accacgaccc gcaccggtaa aaaggggctg
      361 cagggttaca tctatagcac cctttattct gcctaaggcc ccttggttgc tcaataaata
      421 attcaggcca gcctcgccca tctgtttggc gttcgcagga ttggtataag cgagttgaaa
      481 aaactccgct aactcattgg cactctgcag tgttttaagc gcgccgcact gggataagtc
      541 ttcatgcaat tgcaggaaat tatccatatg ggggccggtg gcaataggaa tgccccacag
      601 ggcggcctcc agcatattgt gcccaccacg gggaattaaa ctgccgccga taaacgcagc
      661 agcagccagg ccatataaca gagttaattc tccaagggtg tcgcccaata aaacctgttg
      721 atgaggctta ggtaaatgat cttggctgcg gcgggccagt gtaaaacctt gggcttgaat
      781 taacgctgca accgcatcaa aacgctctgg gtggcggggt actaaaatta ataacgcatt
      841 ggggatgcgt gaaattacct gtttaaatgc agcgagaata atttcatctt ctcccgcatg
      901 ggtgctgcca gcaataaatg taggcctgga atataaaatt gaacgggcgg cctgggcttt
      961 ggaggcgatg gcgctatcta aatgaatatc aaatttaata tttcccacca cctgaatttt
     1021 attccgttta gcgccaaggc tgtgaaaccg ctcggcatgg gcctcggttt gcacggtgag
     1081 catactaata tcgttcatca tttggcagct aagcgcggca aagcgctgat aaccccgtgc
     1141 ggattttgcc gataaacgcc cattggctaa taaactggga atatttttgc ggcggcatac
     1201 gtgcaaccaa ttgggccaga gttcggtttc cataatcacc aggccgcagg gtttggcgct
     1261 tgttaaaaag cgttcaatac aggcgggaat atcatagggc gcccaggctt ggcta
//
LOCUS       DAOQGS010000867         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_82776_length_1308_cov_4.260974, whole genome shotgun sequence.
ACCESSION   DAOQGS010000867 DAOQGS010000000
VERSION     DAOQGS010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82776_length_1308_cov_4.260974"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKZ52_19530"
     CDS             <1..646
                     /locus_tag="PKZ52_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNG62021.1"
                     /translation="IEPAVTQDALQMLSTVKDRFTLLLAEQLHADLLHVRLAALDGRL
                     DIARAQIDEIERVLALENIHNIDMDIERAYIRLAMGKSEEAQALIDFLLQFYAYDQEA
                     LEKLDKLLSEPVSESNRSLVAQINRDGIELYNQAQYDMALAAFAKAQVMFPKHLGIQL
                     NILQCLVGKLRLEPLADEQTVMLHALLGQLSHVVTPEHQQFERFVRLQQMALKE"
     gene            824..>1308
                     /locus_tag="PKZ52_19535"
     CDS             824..>1308
                     /locus_tag="PKZ52_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNG62022.1"
                     /translation="MTLPTEKVDFSLVLAASVHDMKNSIGMLIGSLEQVIDELPPVND
                     VQAQHFNTLQYEASRINGELVQLLTIYRMQNDLLPFRQDQHYVLDVLEDQIARNHVLI
                     ENRHIALSLHCPDNFTWVFDADLVGSVVQNIIVNCCRYTRSRLLVAAQKQDDMLCITI
                     A"
BASE COUNT          307 a          327 c          364 g          310 t
ORIGIN      
        1 aattgaaccc gccgtaaccc aggacgctct gcagatgctc agcacggtga aggatcgctt
       61 caccctattg ctcgccgagc aactacacgc ggatctactg catgtgcgcc tggcggcgtt
      121 ggatgggcgc ctggacattg cccgcgcaca aatcgacgaa attgagcggg tgctcgccct
      181 ggagaacatt cacaacattg atatggatat tgagcgcgcc tacattcgct tggcgatggg
      241 taaaagcgaa gaagcccagg ccctgatcga ctttttgctg cagttctatg cctatgatca
      301 agaggcgctg gaaaaactcg ataaattgct gagcgaaccg gtgagtgagt ccaatcgcag
      361 cctggtggcg cagatcaacc gggatggcat cgagctgtac aaccaagcgc agtacgatat
      421 ggccctcgcc gcctttgcta aggcacaggt gatgttcccc aaacacttag gcatccagtt
      481 aaatatattg cagtgtttgg tgggcaagtt gcgtctggaa ccgctggcgg atgagcaaac
      541 ggtcatgctg cacgccttgc tgggccagtt aagccatgtg gtgacgccgg agcaccagca
      601 gtttgagcgt tttgtgcgct tacagcagat ggcgctgaag gagtagaatg cgcgcccggg
      661 agttttaagc ccctgtttat tttttgcctc aggcaaggca caacaacaat aattaacggc
      721 gcgaacgacc tggcgcctac acatttatag ggtagataga cgcggcttga tgggcgcctc
      781 tatcaaccgg cgctgggttg ctcgatcagc taaggattta ttcatgactc taccgactga
      841 aaaagtagat ttttcattgg tgctcgccgc cagcgtgcac gacatgaaaa actctatcgg
      901 catgttaatt ggctcccttg agcaggttat tgacgagttg ccgccggtaa acgatgtgca
      961 agcccagcat tttaatacgc tgcagtatga ggcctcgcgg attaatggcg agctggtgca
     1021 actgctgacc atctaccgca tgcaaaatga tttattgccc tttcggcagg atcagcacta
     1081 cgtgttggac gtgttagagg atcagatcgc ccgtaaccat gtgttaattg aaaaccgcca
     1141 catcgctttg agcttgcatt gcccggataa cttcacctgg gtgtttgatg ccgatttggt
     1201 gggcagcgtg gtgcaaaata ttattgtgaa ttgctgtcgc tacacccgca gccgtttgct
     1261 ggtggcggcg caaaagcagg atgacatgtt gtgtatcacc atcgccga
//
LOCUS       DAOQGS010000868         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_82908_length_1307_cov_3.488818, whole genome shotgun sequence.
ACCESSION   DAOQGS010000868 DAOQGS010000000
VERSION     DAOQGS010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82908_length_1307_cov_3.488818"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..647
                     /locus_tag="PKZ52_19540"
     CDS             75..647
                     /locus_tag="PKZ52_19540"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNG62023.1"
                     /translation="MATKKPAPAPEAPAAPAAGKSKKRLIIMVVLGVLLIALSVGGTI
                     AALKFMGNKSGDDKKDEHAKEEHHEENNALGATDEKGKPRPTLYLELKPEIVVSFDVA
                     GRQRFLKATVNMVTYDPSIEAAITLHQPMIANAMVMLISKKQFEQLQTVEGKEALRAE
                     AFTEINALMEKETHKKNGITQVLFSEFVLQ"
     gene            660..>1307
                     /gene="fliM"
                     /locus_tag="PKZ52_19545"
     CDS             660..>1307
                     /gene="fliM"
                     /locus_tag="PKZ52_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNG62024.1"
                     /translation="MQDLLSQDEIDALLHGVDDGAVETDIDFDPKAVKTYDLTSQDRI
                     VRGRMPTLEMINERFARYTRISMFNLLRRTADVSVGGIQILKFGEYVHTLYVPTSLNM
                     VKFRPLRGTALFILDAKLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVQMVLQQAFV
                     DLHEAWRAVKKIDFEYINSEVNPSMANIVSPSEVVVVSTFHVELDGGGGEMHITMP"
BASE COUNT          364 a          334 c          310 g          299 t
ORIGIN      
        1 agcttcaccg ccacttggct tggcaccggc tttgctcctg ctgttaatac ccttattttc
       61 gacggaaaat tgacatggca accaaaaaac cggcacccgc gccagaagcc cccgccgcgc
      121 ccgccgcagg taaaagcaaa aaacgcctga tcatcatggt ggtgttaggt gtgttattaa
      181 ttgcgctctc tgtcggcggc accatcgccg cactaaaatt tatgggcaat aagagcgggg
      241 atgataaaaa agacgaacac gccaaagagg aacatcacga agaaaacaac gcattgggtg
      301 ctacggatga aaaaggcaaa ccgcgcccta ccctctattt agaattaaaa ccggaaattg
      361 tggtgagttt tgatgtggcc gggcgccagc gctttttaaa ggccaccgtc aatatggtga
      421 catatgaccc aagtattgaa gccgccatca ccttgcatca acccatgatt gccaatgcca
      481 tggtcatgct catcagcaaa aaacaatttg aacagctgca aaccgtcgag ggcaaagagg
      541 ctctgcgcgc agaagccttc acggaaataa acgctttaat ggaaaaagaa acccataaga
      601 aaaacggcat cactcaggtg ttattttccg aattcgtgct gcaataaagc gagtcttccg
      661 tgcaagattt actctcacaa gacgaaattg atgccctcct tcacggcgta gacgatggcg
      721 ccgttgaaac cgatatagat ttcgacccca aagcggttaa aacctacgat ttaaccagcc
      781 aagatcgcat tgtgcgcggg cgtatgccca cgctcgaaat gatcaacgaa cgctttgccc
      841 gctacacccg catcagcatg tttaatttat tgcgccgcac ggctgatgtt tctgtaggtg
      901 gtattcaaat tttaaaattc ggcgaatacg tccacacgct ctacgtgccc accagtttaa
      961 acatggtgaa attccgccct ctgcgcggca cagccttatt tattctcgat gccaaactgg
     1021 tttttaaatt ggtggacaat ttttttggcg gcgacggcag gcacgccaaa atcgaaggcc
     1081 gcgaatttac cccgacggaa ttgcgcgtag tgcaaatggt actgcaacag gcttttgtgg
     1141 acttgcacga agcttggcgc gccgttaaaa aaattgattt tgagtatatt aattccgaag
     1201 taaacccgtc catggcgaat atcgtcagcc ccagtgaagt ggtggtggtg agcacctttc
     1261 acgttgaact cgacggcggc ggcggtgaaa tgcatatcac catgccc
//
LOCUS       DAOQGS010000869         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_83354_length_1303_cov_3.701122, whole genome shotgun sequence.
ACCESSION   DAOQGS010000869 DAOQGS010000000
VERSION     DAOQGS010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83354_length_1303_cov_3.701122"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKZ52_19550"
     CDS             complement(<1..424)
                     /locus_tag="PKZ52_19550"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNG62025.1"
                     /translation="MQNFTFHNPTRILFGEGQIANIAAHIPAGARILVTYGGGSIKQN
                     GVYDQVAKALEGRTWFEFGGIEVNPHYETLMQATALVKDKNIDFLLAVGGGSVVDGTK
                     LIAAAVYFDGEPWDILAKSAPVTKAMPLACVLTLPATGT"
     gene            complement(937..>1303)
                     /gene="rfbC"
                     /locus_tag="PKZ52_19555"
     CDS             complement(937..>1303)
                     /gene="rfbC"
                     /locus_tag="PKZ52_19555"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNG62026.1"
                     /translation="LHYQLQQAQGKLVRVVQGEVWDVAVDIRRSSPTFGQWVGEILSA
                     ENNRQLWVPAGFAHGFVVLSETAEFLYKTTDYYAPAHERCIAWNDPDLAISWPIDGAP
                     SLSTKDAQGVAFKDADYFA"
BASE COUNT          304 a          300 c          362 g          337 t
ORIGIN      
        1 cagtcccagt ggcgggaagt gttaatacac aggcgagcgg catggctttg gtcactggag
       61 cgcttttggc caaaatgtcc cagggttcgc catcaaaata taccgctgca gcgattaatt
      121 tagtgccatc caccacgctg ccgccgccta cggccagcaa aaagtcgatg tttttatctt
      181 ttactaaagc cgtggcctgc atcagggttt cgtaatgagg gttgacttca atgccgccaa
      241 attcaaacca ggtgcgccct tctagggcct ttgccacttg gtcataaaca ccattttgtt
      301 tgatggagcc accgccatag gtaaccaaaa tacgcgcgcc agcgggaata tgggcggcga
      361 tattcgcaat ttggccttcg ccgaagagga tgcgagtagg attgtgaaag gtaaaatttt
      421 gcatgggctc accctaggtt gttaccggat tgtgtgggcc tagtgtggtg ctgattttat
      481 tttttgcaag tgttccgggg gtgtaatttt gcggttgatt attggatgat aaataagatt
      541 atagttgttt ttacctactc gggtggggga ttaaatttaa taagtggatc ggctggacta
      601 ttcgatatta agtgaagtca tcctttgcgc agtgcctaaa caacctaaag tacttattta
      661 tacgcggcta ccgtttatgg atcctgcgac tgtgcgcagg atgacaagga atattggata
      721 ctgccgctgt gggtaagatg tattaaggga ctaggcgcgt agcccggatg tagcttgtat
      781 agaatccgtg tgggtgaatt catgttcggc gagggaaatg aaacgggcag ccacaagggc
      841 tgcccctaca gatttccacc cgattgcgcg tagtcgcacg atcacatcct actacaaaaa
      901 actcgggctt tacgcaagga tgacactggc gctggattaa gcgaaataat ccgcgtcttt
      961 aaacgctact ccctgggcat ctttggtgga gaggctgggg gcaccatcga ttggccagct
     1021 gatggctaga tctgggtcgt tccaggcgat gcagcgctcg tgggcggggg cgtagtagtc
     1081 ggtggttttg tagaggaatt cggctgtttc ggataacacc acaaacccat gggcaaagcc
     1141 tgcgggtacc cataattggc ggttattttc cgccgagaga atttccccca cccattggcc
     1201 aaaagttggg gagctgcggc ggatatccac ggccacatcc cacacctccc cttgcaccac
     1261 gcgcactaat ttgccctggg cctgttgtag ttggtaatgc aaa
//
LOCUS       DAOQGS010000870         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_83469_length_1302_cov_3.769046, whole genome shotgun sequence.
ACCESSION   DAOQGS010000870 DAOQGS010000000
VERSION     DAOQGS010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83469_length_1302_cov_3.769046"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKZ52_19560"
     CDS             <1..901
                     /locus_tag="PKZ52_19560"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNG62027.1"
                     /translation="QERAQIDGKKHPPFWTRVTLVASAMGVSFVHGSNDGQKGIGLIM
                     LVLIGLAPAQFVLNLDSSKYDIERTYDAAVHLEHFYQDHAALVGSKIDLTTPADAQVA
                     EPFQCTTHNSLVIIGGLKKALHQVDQYRELAVDERRKLRQWLLCIDDSAKKIAKLDGF
                     SSKEISDLNRLRKDITATTEYAPFWVIVAVALALGLGTMTGWKRIVFTVGEKIGKSDM
                     TYSQGMAAQITAATAIGIASATGMPVSTTHVLSSAVAGTMVANRTGLQLSTVKTILLA
                     WVFTLPATIALSAGLFYVGSLFI"
BASE COUNT          306 a          331 c          333 g          332 t
ORIGIN      
        1 ccaggagcgc gcgcaaatag acggcaaaaa acacccgccg ttttggaccc gagtaaccct
       61 ggtggcatcg gccatggggg tgagttttgt gcacggctcc aacgatggcc aaaaaggcat
      121 cggcttaatt atgctggtat taattggcct ggccccggcg caatttgtgt taaacctcga
      181 cagcagcaaa tacgatattg agcgcaccta tgatgccgcc gtgcatttgg agcattttta
      241 tcaggatcac gccgcgctgg tgggcagtaa aattgattta acaacacctg ccgatgcgca
      301 ggttgcggag ccttttcaat gcactacgca caatagctta gttattatcg gcggccttaa
      361 aaaagccttg caccaggtgg atcaataccg cgagcttgca gttgatgagc gccgcaaatt
      421 gcgccaatgg ttattgtgta tcgacgacag cgcgaaaaaa atcgctaaat tagacggctt
      481 tagcagcaaa gaaatttctg atctcaaccg cttgcgcaaa gatattaccg ccaccaccga
      541 atacgcgccg ttttgggtaa ttgtggctgt tgccctggcg ctagggcttg gcaccatgac
      601 gggctggaag cgcatagtat ttaccgtggg tgaaaaaatc ggcaaaagtg atatgactta
      661 ctcccagggg atggccgcgc aaattaccgc cgccaccgcc ataggcatcg ccagcgccac
      721 cggcatgcca gtgtctacca cccatgtttt atcctcagcg gtggcgggca ccatggtggc
      781 gaatcgcacc ggtttgcaac tgagtacagt taaaacaatt ttgctggcct gggtatttac
      841 cctgcctgcc accatagcgc tgtcggctgg attattttat gtggggagcc tatttattta
      901 gcgagttttg cccgctacac agtacttttt actggaactg gacgtgaatc ttgcgccgat
      961 gtgaaaacct caggcgagtg ttttattgat gcgagccagc agtgtattca cccctgctgc
     1021 accgcgcgtt taatttgccg cctgcgccta cgagaataat aaggtgtgcc gaagggctcc
     1081 gtggcaatgt cattcggtta agaattttgg cctgtttttt tgtggcgctt tgttctctgt
     1141 gctaaacccc ctcccggcct cccccttgaa aagggggagg agcaaaaacc ttaagctcgg
     1201 gcactttgct gggcatcaga ccatcttacc tccctctttt aaggcaatat tattaagtta
     1261 ataatattga tctgttgtta gactgttagc gccctttcca ac
//
LOCUS       DAOQGS010000871         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_83585_length_1301_cov_5.495185, whole genome shotgun sequence.
ACCESSION   DAOQGS010000871 DAOQGS010000000
VERSION     DAOQGS010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83585_length_1301_cov_5.495185"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKZ52_19565"
     CDS             complement(<1..530)
                     /locus_tag="PKZ52_19565"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNG62028.1"
                     /translation="MDDFDLRWAPAREKHRGFTLLELLVALIIAAILLAMAVPSFQRM
                     QLSSALQSQGELIFRLTQASRAAALDTATNITLCGTADFTRCQREDFRHLMAFNDGNN
                     SGQREETEPVLYQYSAPANLTMYLRTPPQMPQSWLKYRYAEGDANPYGSFYVCPRNRA
                     MSLGQRVAVSNVGRNR"
     gene            990..1211
                     /locus_tag="PKZ52_19570"
     CDS             990..1211
                     /locus_tag="PKZ52_19570"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNG62029.1"
                     /translation="MKPKFLTLPAVLAAMVLLQGCTHVAPYQRGALAKPEMSWSPDHQ
                     DATLQKHIYFAKEASSGGAEAAGGGCGCN"
BASE COUNT          308 a          364 c          335 g          294 t
ORIGIN      
        1 aagcggttgc ggcccacgtt actcaccgcc acccgctgcc ccaaactcat ggccctattg
       61 cgtgggcaca cataaaacga gccataggga ttggcatcgc cctctgcgta gcggtatttc
      121 agccagcttt gcggcatttg cggtggggtg cgcagataca tggtcaggtt ggcaggcgcg
      181 ctgtattgat acagcacagg ctcagtctcc tcccgctggc cggaattatt gccatcgtta
      241 aacgccatca aatggcgaaa atcctcccgt tggcagcggg tgaaatcggc cgtcccgcag
      301 agggtgatat tggtggccgt atccaaagcg gcggcgcggc tggcctgggt taagcgaaaa
      361 atcagttcac cctgggattg cagtgcactg ctcaactgca tgcgctgaaa actcggcacc
      421 gccatagcca gcaaaatggc ggcgatgata agggccacca gcagttccag taatgtaaaa
      481 cccctgtgtt tttcccgcgc gggtgcccat ctaaggtcga aatcgtccat aaaaattcct
      541 tttacaacca tctgcactca cccgctgcag tgaatttagt gcgcgggcat gtgagctaaa
      601 tgtcggttaa ttctgagctg ggcgctagca tctacgagcg caaaaagcgg tgcgatttgg
      661 ccgctatgag tgtgcgaaat tcaccctagg cgaatccctc acatctcatt tacttttgtg
      721 gtcgctcttg ccatccgctg cgtaatcaac actgcatata cagcaatctc caggcttgct
      781 gaacagataa atctgaacct aaatgagttt gcaggcgctc gggccaatac gcctgctggc
      841 aaagtgtgaa aaaccgctat tcgtgcactc aacagcccac aaattctgcg ccatctgctt
      901 taggggggct ggcctaagct gctagaatgc gcgggatttt atgcctgccg taaatcggtg
      961 agtacaacgc ggtaatagtg agaacatcta tgaagcctaa attcctaaca ctacctgccg
     1021 tgttggcagc gatggtactg ctacaaggct gcacccatgt ggcaccttat cagcgcggcg
     1081 cgctggccaa gcctgaaatg agctggagcc cggatcacca agacgccact ttacaaaaac
     1141 acatttactt cgctaaagaa gcttcctccg gcggtgcaga agctgccggt ggtggatgtg
     1201 gttgcaacta gggaaacgct cattcatgac taataccact tttaaaaaac cttcccagcg
     1261 tctcctagcc ctttccactg ccgccatggc actgccggct t
//
LOCUS       DAOQGS010000872         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_85155_length_1288_cov_3.424169, whole genome shotgun sequence.
ACCESSION   DAOQGS010000872 DAOQGS010000000
VERSION     DAOQGS010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85155_length_1288_cov_3.424169"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..>1288)
                     /locus_tag="PKZ52_19575"
     CDS             complement(129..>1288)
                     /locus_tag="PKZ52_19575"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNG62030.1"
                     /translation="TPFESIIVETDDSSATASFYVDNLSVKITAAPTLDTGNGLAANA
                     DFPVGVALAADGNASIFTSVPRQNIVKANFDEIVAENIMKMSYMYSGSAFSFTNADNL
                     ITWATDAGLKVHGHALVWHASYQLPAWATINNPTFKQDFATHIDTVAEHFAGKVVGWD
                     VVNEALFDAADDSSGLGSANGYRQSVFYQAYNGPSFIDEAFTRARAKDPSAVLYYNDF
                     NTEANGDKTTALVNMVQGLLDRNVPIDGVGFQMHVLNDYPSIANIRASLQKIVNLSPT
                     LKVRISELDVRINNPYDGNSNNDYKNREDCVFYCPGLATQKARYKEIVQAYLDIVPPA
                     QRGGITVWGIADPDSWFYSNNGGLPDWPLMFNDSLQAKPAFLGVQEALKGI"
BASE COUNT          334 a          296 c          310 g          348 t
ORIGIN      
        1 cttgctatgg agttttaaat aaaactatat aggcccgagc attctattaa attaggccca
       61 taaaaaaacc cgcatgtttc tatgcgggtt ttggcaggtt ttggcgggtt aaattaataa
      121 agcgtgcgtt aaattccttt aagcgcctct tgcaccccta agaaggcagg cttagcctgc
      181 aggctgtcgt taaacatcaa cggccaatcg ggcaaaccac cattgttgga ataaaaccag
      241 ctgtcggggt cagcaatacc ccatacggtg attccgcccc gttgagcagg gggtacaatg
      301 tctaggtagg cctgcacaat ttctttataa cgggcttttt gtgtcgctaa acctgggcaa
      361 tagaaaacac agtcctcgcg gtttttatag tcgttattgg agttgccgtc ataggggtta
      421 ttaattcgca catccaattc ggaaatacga actttaagag tggggcttag attgacaatt
      481 ttctgcaagg atgcacgaat attggcgatg ctcggataat catttagcac gtgcatttga
      541 aaacctacgc cgtcgatagg tacatttctg tccaggagtc cttgcaccat attcactagg
      601 gctgtggtct tgtcgccatt ggcttcggtg ttaaagtcgt tgtaataaag caccgcactg
      661 gggtctttcg cccgggcacg ggtgaaggcc tcatctataa acgacgggcc gttataggct
      721 tggtaaaaca cagattggcg ataaccattg gctgaaccca gtccagaaga atcgtccgct
      781 gcatcaaaca atgcttcgtt aaccacatcc cagcctacaa ctttgcctgc aaaatgctcc
      841 gccactgtat caatatgggt agcaaagtct tgtttaaagg tagggttgtt gatcgttgcc
      901 catgcgggta attgataact cgcgtgccat accaaagcgt ggccatgcac tttcaggcct
      961 gcatcggttg cccaggtaat aaggttatca gcattggtaa acgaaaacgc cgagcccgaa
     1021 tacatgtaac tcatcttcat aatgttttca gccacaattt catcgaaatt ggctttcaca
     1081 atattttggc gaggaacgct ggtgaaaata ctggcgttgc catctgcagc taaggcgaca
     1141 cctactggga agtccgcatt agctgccagt ccatttccgg tatccagagt gggcgctgcg
     1201 gttatcttga cggagagatt gtccacataa aaactcgcgg tagcactgct atcgtcggtt
     1261 tctactatga tcgattcaaa tggagtgg
//
LOCUS       DAOQGS010000873         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_85529_length_1285_cov_3.720325, whole genome shotgun sequence.
ACCESSION   DAOQGS010000873 DAOQGS010000000
VERSION     DAOQGS010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85529_length_1285_cov_3.720325"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1285)
                     /gene="clpX"
                     /locus_tag="PKZ52_19580"
     CDS             complement(<1..>1285)
                     /gene="clpX"
                     /locus_tag="PKZ52_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018277196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNG62031.1"
                     /translation="FCGTPKTAEVPLIAGNDGHICEQCIKLAYQVVSSWGRKAAAVKP
                     ELKTPVAIKSFLDEYIVGQEQAKRTLAVAVYNHYLRFNAQANTQPFAHIHTIAGVELE
                     KSNVLLAGPSGSGKTLLVKTLARIIGVPIVVGDATSLTQAGYVGEDVDSLLKRLLDAA
                     NGDVQQAQWGIVYIDEIDKLARRGAGGSAVRDISGEGVQQALLKMVEGSEVRLPKAGR
                     RDGHSEDVVMNTSNILFIVGGAFSGLTELISKRLKPAIVSIGFNAKVEKDAAATNDEL
                     LAALQPDDLHQFGLIPEFIGRFPIVTFLQDLDVEALKSILKDPRNALTKQYHHLFALQ
                     GVDLHFSDEAITLIAQQAVTRGTGARGLRAILEGLLCNTMFNLPTTANLTRVAVNTRL
                     VEEEEQLVVDEIIGEGEAVNKPKNLGFIEENTAVES"
BASE COUNT          247 a          347 c          382 g          309 t
ORIGIN      
        1 gctttccact gccgtgttct cttcaataaa gccgagattt ttaggcttat taacggcttc
       61 gccctcacca attatttcat ccactaccaa ttgttcctcc tcctccacca ggcgggtatt
      121 caccgcaacg cgggttaaat tggcggtggt gggcaggttg aacatggtgt tgcacagtag
      181 gccttctaaa atcgcccgca ggccccgagc gccagtgccg cgggtgacgg cctgttgggc
      241 gatgagggtg atggcttcgt cgctaaagtg caggtccacg ccctgcaggg caaacaggtg
      301 gtggtactgt ttggtaagag cgttgcgcgg gtctttgagg atagatttaa gggcctctac
      361 atccaggtct tgcaggaagg tcactatggg gaagcggccg ataaattcgg ggattaagcc
      421 aaattgatgc aggtcgtccg gttgcagggc ggccagtagt tcatcgttgg tggcggcggc
      481 gtctttctcc actttggcgt taaagccgat gctgactatg gcgggtttga ggcgtttgct
      541 gatgagttcg gtaaggcctg aaaaggcgcc gccgacgata aataaaatat tgctggtgtt
      601 catcaccaca tcttctgagt ggccgtcccg gcgaccggcc ttaggcaggc gcacctctga
      661 accctccacc atttttaaca gcgcctgttg cacgccttca ccggaaatat cccgcaccgc
      721 agagccgcca gcgccccggc gggcgagttt gtcgatttca tctatgtaca caatgcccca
      781 ttgggcctgt tgcacatcgc cattggcggc atccagcagg cgtttaagta ggctgtccac
      841 gtcttcaccc acataaccgg cctgggtgag ggaggtggca tcccccacca ctatgggcac
      901 gccgataatc cgcgcgaggg tttttaccaa cagggtttta cccgagcctg agggaccggc
      961 cagtagcacg ttggattttt ccagctccac tccggcgatg gtgtgaatat gggcgaaggg
     1021 ctgggtgttg gcctgggcgt taaagcgcag gtagtggttg tacaccgcta ccgccagagt
     1081 gcgcttggcc tgttcttggc cgacgatgta ttcatccaaa aaggatttaa tggccactgg
     1141 ggtttttaat tcgggcttca cggcggcggc cttgcggccc cagctgctca ctacctggta
     1201 ggcaagtttg atgcactgct cgcagatatg gccgtcgttg cccgcaatca gcggcacttc
     1261 tgcagttttg ggggttccgc aaaag
//
LOCUS       DAOQGS010000874         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_85759_length_1283_cov_5.022801, whole genome shotgun sequence.
ACCESSION   DAOQGS010000874 DAOQGS010000000
VERSION     DAOQGS010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85759_length_1283_cov_5.022801"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKZ52_19585"
     CDS             complement(<1..565)
                     /locus_tag="PKZ52_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNG62032.1"
                     /translation="MTRFLFLGVMAFILELLFSSFASAAGPLGDTPNFSRIPFNASDS
                     IGEIITISQDDLGFLWLGGRRGLARYDGYRIHTYKANPRDPKALGDDRITHVFKDSYG
                     ELWVATASAGVARLNRNQDNFHTYKIETSDGGKTRQQNFTEILEDEQRNLWIIGGEGI
                     ALYNRAQNTFTRHLTHSPIINSPILKML"
     gene            complement(810..>1283)
                     /locus_tag="PKZ52_19590"
     CDS             complement(810..>1283)
                     /locus_tag="PKZ52_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNG62033.1"
                     /translation="DIAFAAALAELGVTINYGDNWVEASLPKGQPLKAFNRDFNHIPD
                     AAMTLALVALFCDGPCRLNNIASWRVKETDRIAAMATELRKLGAQVEEGPDWLAVHPL
                     AQLLPNASIDTYDDHRMAMCFSLASLAGVPVRINDPKCTAKTFPTYFEVFKSVVR"
BASE COUNT          312 a          302 c          355 g          314 t
ORIGIN      
        1 cgagcatttt taaaataggg ctgttaataa tcggcgaatg ggttagatgg cgggtaaatg
       61 tgttctgggc tcgattgtat agggctatac cttcgccgcc gataatccac aggttgcgct
      121 gctcgtcttc aagtatctcc gtaaaatttt gttggcgggt tttgccgccg tcgctcgttt
      181 cgattttata agtgtgaaag ttatcttggt tgcggtttaa acgggcaacc cctgcgctgg
      241 cggtggcaac ccataattcc ccgtaggaat ccttaaatac atgggtaatg cgatcatcgc
      301 ccagcgcttt agggtcgcgg ggattcgcct tgtaggtgtg tatacggtag ccatcgtagc
      361 gcgctaaacc tcggcgcccg cccagccaga gaaaacctaa atcatcttgg ctgatggtaa
      421 taatttcacc aatcgaatca ctggcgttaa atgggatgcg cgaaaaattg ggcgtgtcgc
      481 caaggggccc ggcagccgaa gcaaaagaac taaacaataa ctcgaggata aaagccatta
      541 ccccaaggaa taaaaaacgc gtcattcgta attggccaat ggcatcagag caaaatcaac
      601 ctacagctgt gttgtggtca ataatgagaa tagcctgagg cgagaggatc tgctgtaaat
      661 gccgataaaa tggccgtttt atagcgaata gttggccctt tgggtatttt tagaatggga
      721 taattttggc gttctttgcc aatgaattaa ttcagcaaca cttccctgtg atcacatcac
      781 ttattgcgcg gtgccaatct aatgcgccac taacgcacta cacttttaaa cacttcaaaa
      841 taggtgggaa aggttttggc ggtacatttc gggtcgttaa tacgcaccgg aacaccggcc
      901 aggctcgcga gagaaaaaca catggccatg cggtggtcgt cataggtgtc gatactggcg
      961 ttgggtagga gctgcgcaag tgggtgcacc gccagccaat ccgggccttc ttccacctgg
     1021 gcgcctaatt tgcgcagctc agtggccatg gcggcaatgc ggtcggtctc ttttacccgc
     1081 cagctggcga tattgttcag gcggcagggg ccgtcgcaga agagcgccac cagggcgagg
     1141 gtcatggcgg catcggggat gtggttgaag tcgcggttaa aggctttaag cggttgcccc
     1201 ttgggcagac ttgcctccac ccagttatcg ccgtaattga tggttacacc cagttcggct
     1261 agggcagcag caaaggcaat atc
//
LOCUS       DAOQGS010000875         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_85870_length_1282_cov_4.521597, whole genome shotgun sequence.
ACCESSION   DAOQGS010000875 DAOQGS010000000
VERSION     DAOQGS010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85870_length_1282_cov_4.521597"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1278)
                     /locus_tag="PKZ52_19595"
     CDS             complement(322..1278)
                     /locus_tag="PKZ52_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNG62034.1"
                     /translation="MDPATLAQAHQLVHKTQQEITQVVLGQDAVVQQVLIALLARGHV
                     LLEGVPGLGKTLLVRALAKCFGGEFRRIQFTPDLMPSDVTGHALFNMQSQAFEIRKGP
                     VFTHLLLADEINRAPAKTQAALLEVMEEQQVSIEGESLPVAAPFMVLATQNPVEQEGT
                     YPLPEAQLDRFLLKVLIDYPSQAHEELLVKTMGKTRTPLEAIRTFLTPAHISSLQKVC
                     SQITLDDAVLGYIVQLVRATRQWPGLERGAGPRAGLGIAAAAKAFALINERAYVTPND
                     VKTLAPAVLRHRISLTADAEIEGLSAESIIADIIQQTAAPRQ"
BASE COUNT          239 a          341 c          387 g          315 t
ORIGIN      
        1 ggcggccatg gggttggaaa aaattgattt tatttggtac ctgcgcaaag tgagcctgct
       61 ggcgttaatg ggttattttg ccggtgcggg tgtttatatt ttgcaaagca gtttggtgta
      121 attttaagtt ggccgtgcag caggtatttt taattatgct aaaaaccgca gcggcgtagg
      181 ttgggcagta atgcccaaca caactaattc ctcgcaagca cgccgccaat ttcaacgcgt
      241 cgtctcccga ccattttttt cgacgacacc gttgtgttag gcattactgc ccaacctacg
      301 ccgctaatgt agccaccgta ttcactgcct gggcgcggcg gtctgttgaa taatatcggc
      361 aataatgctc tcggcgctta ggccttcaat ttccgcatcg gcagtgaggc tgatgcggtg
      421 gcgtagcacc gccggggcga gggttttaac atcgttgggg gttacatagg cccgctcatt
      481 gatcagtgcg aaggccttgg ctgccgcggc aatccccaac cccgccctgg gccctgcgcc
      541 gcgctcaagg cccggccatt ggcgcgtggc gcgcactaac tgcactatgt agcccagcac
      601 cgcgtcatcc agggtgattt ggctgcagac tttttgcagg ctgctgatgt gggcgggggt
      661 taaaaaggtg cggatggctt ctaagggtgt gcgggttttg cccatggttt ttaccagtag
      721 ctcctcgtgg gcttggctgg ggtagtcgat taataccttg agtaaaaacc ggtccagctg
      781 cgcctctggc agcggatagg tgccctcctg ttcaaccggg ttttgggtgg ccagcaccat
      841 aaacggggcg gctaccggca gggattcccc ctcaatgctc acctgctgtt cttccattac
      901 ctccaacagg gccgcctggg ttttggccgg ggcgcggttg atttcatcgg ccagcaacag
      961 atgggtaaac accgggcctt tgcggatttc aaacgcctgg ctttgcatgt tgaacagtgc
     1021 gtggccggtg acatcgctgg gcattaaatc cggtgtgaat tggatgcggc gaaactcgcc
     1081 gccaaaacat ttggccagtg cccgcaccag cagggttttg ccaagccccg gtacgccttc
     1141 taacaacaca tggccgcggg cgagcagggc gataagcacc tgttgcacca ccgcgtcttg
     1201 gcccaacacc acttgggtaa tctcctgctg ggttttgtgt accagttggt gggcttgggc
     1261 gagggtggcc gggtcgatgg tt
//
LOCUS       DAOQGS010000876         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_88360_length_1262_cov_4.301574, whole genome shotgun sequence.
ACCESSION   DAOQGS010000876 DAOQGS010000000
VERSION     DAOQGS010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88360_length_1262_cov_4.301574"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKZ52_19600"
     CDS             <1..788
                     /locus_tag="PKZ52_19600"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNG62035.1"
                     /translation="FLHGGAAINGFCAEAGLTLWVVDAGVNAELPAHPLLKAAKIAHG
                     TEDYCQQAAMSAAQLELAFSRADALVAELAASGCNLLALGEMGIGNTASSALITQVLT
                     GIALKDLVGPGTGLDKVGVAEKLRRLEAALNLHQLIQPSGERALQVFGGFEMAMLAGA
                     CLSAAARGMLVLVDGFIASAAVLAAVKINSNALAYCVFCHTSSEPGHRLLLDHLQVKG
                     LLDLGLRLGEGSGAALAYPLVKAAARFINSMASFESAGVSRAI"
     gene            778..>1262
                     /locus_tag="PKZ52_19605"
     CDS             778..>1262
                     /locus_tag="PKZ52_19605"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNG62036.1"
                     /translation="MQFKSELLSREIHYFFNAWVFFTRFPAPKWVQFNDEHLQQGARY
                     FSLIGLFLGLLCAGCFYLFSLVFTVDLAVLLTLVIGIFATGAFHEDGWADCCDGFGGG
                     YAKTQILTIMKDSRLGTYGVVGLIGLLATKYLALLNIAQFDMGLGWVLVCGFAASRFM
                     AT"
BASE COUNT          239 a          328 c          360 g          335 t
ORIGIN      
        1 attttttgca cggtggcgcc gccattaacg gcttttgcgc cgaggccggt ttaaccctgt
       61 gggtagtaga tgccggggtg aacgccgagc tgcccgcaca ccccttgtta aaagcggcaa
      121 aaattgccca tggcaccgag gattattgcc agcaagcggc catgagcgcc gcccagttag
      181 aactcgcctt tagccgcgcc gacgccctgg tggcggaatt agccgccagc ggctgcaacc
      241 tgttggccct gggggagatg ggtatcggca acaccgccag cagcgcactt attacgcagg
      301 tattaaccgg cattgccctt aaggatttag ttggcccagg cacgggcctt gataaggtag
      361 gggttgccga aaaattgcgg cggcttgagg ccgcactcaa ccttcaccaa ttgatacagc
      421 ccagcgggga gcgcgccctg caggtttttg gcggctttga aatggccatg ttagcgggcg
      481 cctgcttaag tgcggcagcg cggggaatgc tggtgctggt ggacggattt atcgccagtg
      541 cggcggtttt agcggcggtg aaaatcaata gcaatgcact ggcttattgt gtattttgtc
      601 acacctcctc agaacccggg caccggcttt tgttagacca tttacaggtc aaaggcctac
      661 tggatttggg tttacgcctt ggggagggca gcggcgccgc cttggcctat ccccttgtaa
      721 aagcggctgc gcggtttatt aattccatgg cctctttcga atcggcaggt gtcagtcgtg
      781 caatttaagt ccgaattgct ttcacgagaa attcattatt tttttaatgc ctgggtattt
      841 ttcacccgct tccccgcgcc caaatgggtg cagtttaatg acgagcacct gcaacagggc
      901 gcccgttatt tttccctcat tggtttgttt ttaggcctct tgtgcgccgg ctgtttttac
      961 ctgtttagcc tggtatttac cgttgattta gccgttttac tcaccctggt gataggcata
     1021 tttgcgaccg gtgcatttca cgaagacggc tgggccgact gctgcgacgg ttttggcggc
     1081 ggctacgcca aaacgcaaat tctcaccatc atgaaagact cccgcctggg cacctatggc
     1141 gtggtgggtt taattggttt attggccact aaatatttgg ccctgttaaa tatcgcccaa
     1201 tttgatatgg gcctgggctg ggttttagtc tgcggttttg ccgccagccg ttttatggcc
     1261 ac
//
LOCUS       DAOQGS010000877         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_89350_length_1254_cov_4.106756, whole genome shotgun sequence.
ACCESSION   DAOQGS010000877 DAOQGS010000000
VERSION     DAOQGS010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89350_length_1254_cov_4.106756"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PKZ52_19610"
     CDS             <1..881
                     /locus_tag="PKZ52_19610"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNG62037.1"
                     /translation="LVLDGLAPKIIALPRNIARDASAAFKRLNEYCQADPLCQQHYGD
                     LALNAQTITRHLQLAEEANKPEHITLPDPLNQKPREWIFTPRRFAQLLISALYSRELT
                     SLAPRALKEAADGNYQLLGTLSALAEANSEQLSIAEGLYYSIICNEDLAAAPQAPAPE
                     PIFMGVNLVEDKLAVCANWPKTAPLANSISNPQINQTPTFLLSGALDPVTPKPWADLV
                     ALTQLNHQMLEVPGGNHVVSTLGCIPNLIAQFINTPSARLPTECSQAIAPLPFYLGAD
                     PAAQEPHDLTQEARHD"
     gene            874..>1254
                     /locus_tag="PKZ52_19615"
     CDS             874..>1254
                     /locus_tag="PKZ52_19615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG62038.1"
                     /translation="MIDIQRLSKHFTSKNGAPITALNQLTTQFHDGAITAILGLNGAG
                     KSTLMRIIYGLISATEGDVHIGGYSISSQANQARRTMGCLADDTGLYTRLSARENIQY
                     FGRLHGLSPKEITPYCDELIDLLGM"
BASE COUNT          323 a          357 c          283 g          291 t
ORIGIN      
        1 gtttggtgct cgatggcctt gcgcccaaaa ttatcgccct gccccgcaat attgcccgcg
       61 atgcaagcgc agcctttaag cgcctgaatg aatattgcca ggcggacccg ctttgccagc
      121 agcattatgg tgatttggcc ctcaacgcgc agactattac ccgtcattta caactggccg
      181 aagaggcgaa taaacccgaa cacatcacct tgcccgaccc gttaaatcaa aaacccagag
      241 aatggatttt tacgccgcgt cggtttgcgc aattattaat ttccgccctt tattcccgtg
      301 aattaaccag cctcgcgccg cgggcattaa aagaagccgc cgatggcaat tatcaattat
      361 taggcaccct aagcgcttta gcggaagcca acagcgagca gctctccatt gcagaaggct
      421 tgtattacag cattatttgc aatgaagact tggcggctgc cccgcaagcc ccagcgcctg
      481 agccaatttt tatgggcgta aatttagttg aggataaatt agcggtatgc gccaactggc
      541 ccaaaacagc gccgcttgcg aactcaatat ccaaccccca aatcaaccaa acaccgactt
      601 ttttattgtc cggcgccttg gacccagtta cccccaaacc ctgggccgat ctggtggcgc
      661 tgacccaatt aaatcaccag atgctggagg tgccaggcgg caaccacgtg gtatcaaccc
      721 taggctgcat acccaattta attgcgcaat ttattaatac acccagcgcg cggctaccca
      781 ccgaatgcag ccaggccatt gccccattgc ccttttattt aggcgcagat cctgcggccc
      841 aggagcccca tgatttaact caggaggccc gccatgattg atatacagcg cttgagtaaa
      901 cattttacga gcaaaaatgg cgcaccaatc acggcattaa atcaattaac cacacaattt
      961 cacgatggcg ccattaccgc tattttaggg ttgaacggcg cgggtaaatc cacattaatg
     1021 cggattattt acggtttaat ttcagccact gaaggtgatg tgcatatcgg cggttattcc
     1081 attagctccc aagccaatca agcccggcgc accatgggct gtttagcgga cgacaccggc
     1141 ctttatacac ggctcagcgc gcgggaaaat attcagtact tcggccgttt acacgggctt
     1201 agccccaaag agattacacc ttattgcgat gaacttattg acctgctggg aatg
//
LOCUS       DAOQGS010000878         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_89594_length_1252_cov_5.000835, whole genome shotgun sequence.
ACCESSION   DAOQGS010000878 DAOQGS010000000
VERSION     DAOQGS010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89594_length_1252_cov_5.000835"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /gene="eda"
                     /locus_tag="PKZ52_19620"
     CDS             complement(<1..504)
                     /gene="eda"
                     /locus_tag="PKZ52_19620"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNG62039.1"
                     /translation="MTITRDYLTPIGVMPVIVIDSVEHGVPVAEALYAGGIKAVEITL
                     RTPAALGAIKAIKKACPHIIVGAGTLVNEKNVEEVAEAGVDFAVSPGMTVSLIKKSAE
                     CGVKLLPGVATCSEILLGMEHGLSCFKLFPATAVGGLPLLKSIYGPLPQVSFCPTGGL
                     TLDDFTQY"
     gene            complement(560..>1252)
                     /locus_tag="PKZ52_19625"
     CDS             complement(560..>1252)
                     /locus_tag="PKZ52_19625"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNG62040.1"
                     /translation="GLDAYEKAPSLDDKGNAVWLEPVTQSSDTSILRSVDGPFDSEGG
                     LRLLKGNLGQGVIKISAVAPEYRVVEEECIVFDDQDDLIAAFKRGELNRNFVAVVRFQ
                     GPRANGMPELHKMTPPLGLLQDRGYKVALVTDGRMSGASGKVPSAIHLSPEALCGGIL
                     TRVRTGDRVRLDANTGSIDVLVSEEELAERIPVTQPQRAQDLGRNLFGGFRSLADLAS
                     EGGSVFNSCIDF"
BASE COUNT          338 a          352 c          280 g          282 t
ORIGIN      
        1 atactgggtg aaatcatcca gcgttaaacc gccagtgggg caaaagctca cctgcggcag
       61 gggcccataa atagatttta atagcggcaa accgcccact gccgtggcgg gaaataattt
      121 aaaacagctt aggccgtgct ccatgcccaa taaaatttct gagcaggtag caacaccggg
      181 caataatttc acgccgcatt cagcggattt tttaatgagg cttaccgtca tgccggggga
      241 aacagcaaaa tcaacacccg cttcggccac ttcctcaaca tttttttcat tcaccaaggt
      301 gcctgcgccc acaataatat gcgggcaggc ctttttaatg gctttaatag cacccagggc
      361 tgctggggtg cgcagggtaa tttcaacggc tttgatgccg cctgcgtaca gcgcttcggc
      421 aactggaact ccatgctcaa cagagtcaat tacaattact ggcatcacgc caatgggagt
      481 taaataatcg cgtgtaatag tcacggcaca ctcacttttt taaatgaaaa aaataaaggc
      541 agcttttgct gccctttaat taaaaatcga tacaggagtt aaatacactg ccgccttcac
      601 tggcaaggtc agctaaactg cggaagccac caaataaatt gcggcccaaa tcctgcgcgc
      661 gctgcggttg agtgacggga attcgctccg ccaattcttc ttcactcacc aacacatcaa
      721 tcgagccggt attggcatcc aaacggacgc gatcccccgt gcgcacacgg gtcagtatcc
      781 cgccgcagag cgcctcaggc gataaatgga tcgccgaggg cacttttcca gaagcaccgg
      841 acatgcgccc atcagtcact aaagccactt tataaccacg atcttgcaac agccccaaag
      901 gcggcgtcat tttgtgtaat tccggcatgc cgttggcgcg ggggccttga aaacgcacca
      961 ccgcaacaaa attgcgattt aattcaccgc gcttaaacgc cgcgattaaa tcgtcttgat
     1021 cgtcgaacac aatacattct tcttccacca cgcgatattc aggcgccacg gcggaaattt
     1081 taattacgcc ctgccctaaa ttacctttta acaagcgcag gccaccttct gaatcaaatg
     1141 ggccatccac gctgcgcaaa atagaagtgt ccgaagattg tgtaacaggc tccaaccaaa
     1201 cggcgttgcc tttgtcatcc aggctcggcg ctttttcata ggcatctaag cc
//
LOCUS       DAOQGS010000879         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_92456_length_1231_cov_4.289966, whole genome shotgun sequence.
ACCESSION   DAOQGS010000879 DAOQGS010000000
VERSION     DAOQGS010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92456_length_1231_cov_4.289966"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKZ52_19630"
     CDS             complement(<1..442)
                     /locus_tag="PKZ52_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNG62041.1"
                     /translation="MKQRIWVLGAGQLGAMLKHAGMPLALEVCPVDIDDHTAPALAAC
                     DWVTAERELWPQTLATQTLAGHPNFVNKDVFGRLADRKTQKELIDSLNIKTAPWRLVA
                     GDITAAQLRADLGEKVLLKRRTGGYDGRGQHWLKTPADAVPDDWQ"
     gene            complement(453..947)
                     /gene="purE"
                     /locus_tag="PKZ52_19635"
     CDS             complement(453..947)
                     /gene="purE"
                     /locus_tag="PKZ52_19635"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNG62042.1"
                     /translation="MANKVAVIMGSQSDWSTMQAAVEVLEQMGVECTAEVVSAHRTPH
                     KLSEFAESAADKGYAVIIAGAGGAAHLPGMVAAHTRLPVLGVPVQSKALSGVDSLYSI
                     VQMPKGVAVGTLAIGVAGAFNAGLLACQILALTDPALAQKIEQFRTQQTQAVLDNPDP
                     RAQS"
BASE COUNT          261 a          342 c          342 g          286 t
ORIGIN      
        1 cttgccaatc atcgggcacc gcatcagcag gagtttttaa ccagtgttgg ccgcggccgt
       61 cgtaaccgcc ggtgcggcgt ttaagcagca ccttttcccc taaatcagcg cgcaattgcg
      121 cagctgtaat gtcgcctgca actaaacgcc agggggcggt tttgatattt aaagaatcaa
      181 ttaattcttt ttgggttttg cgatcggcca ggcggccaaa tacatcttta ttcacaaaat
      241 tagggtgtcc ggcaagggtt tgggtggcca gggtctgggg ccataattcc cgctcggcgg
      301 ttacccaatc gcaggcggca agggcggggg cggtgtggtc atctatatcc accgggcaca
      361 cctctaaggc cagcggcata cccgcgtgtt ttaacatggc gcccaattgg cccgcgccga
      421 ggacccaaat tcgctgtttc atggagctgc ccttaagact gcgcgcgggg gtctgggtta
      481 tccagcaccg cttgggtctg ttgggtgcgg aactgctcga ttttttgcgc cagggcaggg
      541 tcggtaagcg ccagaatctg gcaagccaac aggccagcat taaaggcacc ggccacacca
      601 atagccaagg tgcccaccgc cacgcccttg ggcatttgca ctatggaata caggctatcc
      661 acgccgctta aagccttgct ttgcaccggc acacccagca ccggcaagcg cgtatgagcc
      721 gccaccatgc ccggcaaatg cgccgccccg ccagccccgg cgattatcac cgcatagccc
      781 ttatccgctg cggattcagc aaattcgctg agtttatggg gggtgcggtg ggcggacacc
      841 acctcagcgg tgcactccac acccatctgc tctaagactt ctaccgccgc ttgcatggtg
      901 ctccaatcgc tctgggagcc catgataact gccactttgt ttgccatagt gcgtacctaa
      961 atcaatacgg gcctagcttg gccggaatta aaaaggcgcg gattttagca taggtggtgg
     1021 gaaggcaacg ccattatgtt gagtatgctt cgattgaaat agccgtaggg gcgccccttg
     1081 tgggtgccct tcctcgtgta attctgaagg gaagttggag gtaaaaactg ggagtaactt
     1141 attacctggc ggcaaaatag atcgttctga tctactttgc cttcgctgcg ttaaacttac
     1201 gaaaatgcgt ggattttctt gttttaacgc a
//
LOCUS       DAOQGS010000880         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_92723_length_1229_cov_4.179727, whole genome shotgun sequence.
ACCESSION   DAOQGS010000880 DAOQGS010000000
VERSION     DAOQGS010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92723_length_1229_cov_4.179727"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..887)
                     /locus_tag="PKZ52_19640"
     CDS             complement(144..887)
                     /locus_tag="PKZ52_19640"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNG62043.1"
                     /translation="MPHTANKPFYLVGHRGLPAYYPENTLAGILAAIAEGADAVEFDI
                     QISKDNIPVVIHDDTLLRTTGINGDLADYTYSLLERISAHEPERLGQSHLPEPIVSLA
                     KLVQELKKWPEVHAFAEIKSEIFKRQTREKTLHSIATILTPIKSHCSIISFDYEILSL
                     ARAAGFSIGWVLNAMDETSLACALALAPEFLIIDYNQIPPSGPWPGPWRWFVYDITDG
                     PSAAAAIKKGITYIESWDARGLKQYFDAL"
     gene            complement(895..>1229)
                     /locus_tag="PKZ52_19645"
     CDS             complement(895..>1229)
                     /locus_tag="PKZ52_19645"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNG62044.1"
                     /translation="QAAVNACAHNCAQWGLAAESVLADCGDGINQRFDLILCNPPFHQ
                     GFNVENQLTQRFLAAAAKRLNTTGAAFFVVNQFIALERKAGEYFKHCELKSSDGRFKV
                     LRLTQPKI"
BASE COUNT          303 a          265 c          335 g          326 t
ORIGIN      
        1 cgccgtgaat taatttggag ctattggcag atgtggcagc gcccacacgg ttgttttttt
       61 caattaataa gcagttaagc cccagtgcct gtgcaatgta gctaaagcct gcgccgtgga
      121 tgccagcgcc gataataatt aggtcatagt gcgtcaaaat actgttttaa cccgcgtgca
      181 tcccagcttt caatataggt gatgcctttt ttaatggctg cagcagcgct tggcccatca
      241 gtaatatcgt agacaaacca gcgccagggg ccgggccaag ggccgcttgg cggaatttgg
      301 ttgtagtcga taattaaaaa ctcaggtgcg agggctaggg cgcaggcgag ggaggtttca
      361 tccatggcgt ttaataccca gccgatgctg aaacccgccg cgcgggcgag gcttagaatt
      421 tcgtagtcaa aagaaataat gctgcagtgg gatttaatgg gggtgagaat ggtggcaatg
      481 ctgtgcaggg ttttttcgcg agtttggcgt ttaaaaatct cgcttttaat ctctgcaaaa
      541 gcgtgcacct ccggccattt ttttaattct tgaactaatt tggcgaggct tacaataggc
      601 tcgggcaaat ggctttggcc taggcgttca ggctcgtggg cgctgatgcg ctctaaaagg
      661 gaataggtgt aatcggctaa atcaccgtta ataccggtgg tacgcaacaa agtgtcgtcg
      721 tgaataacca ctggaatatt gtctttgcta atttgaatat caaattctac tgcgtcggcg
      781 ccttcggcta tggcggcgag aattcccgct agggtatttt cagggtaata ggcgggtagg
      841 ccccggtggc cgactaaata aaaaggttta ttggcagtat gcggcataat aatcctaaat
      901 tttaggctgg gttaagcgca gcactttaaa gcgaccatcg ctgcttttaa gctcacagtg
      961 tttaaaatat tccccggctt tgcgctctag agcaataaat tggttgacta caaaaaaagc
     1021 cgcgccagta gtgttaagcc gtttagcggc ggcggctaaa aaacgctggg tgagttgatt
     1081 ttctacatta aaaccttggt gaaaaggcgg gttacagagg attaaatcaa agcgttggtt
     1141 tattccatcg ccgcaatcgg ccaatacgct ttcggctgct aggccccatt gggcgcaatt
     1201 gtgtgcgcag gcatttaccg ccgcctggt
//
LOCUS       DAOQGS010000881         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_93025_length_1227_cov_4.603242, whole genome shotgun sequence.
ACCESSION   DAOQGS010000881 DAOQGS010000000
VERSION     DAOQGS010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93025_length_1227_cov_4.603242"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKZ52_19650"
     CDS             <1..368
                     /locus_tag="PKZ52_19650"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNG62045.1"
                     /translation="LLILPVVFIAQPIMAAIFDRIVDARYGSLAASLLLFLLPSIVMG
                     MVSPYSVRLLTQSRESSGASAGVLYFVSTLGSALGTLATSFYLVALWEINQILLAAVA
                     VFCLNGLLLIAAHSRMPVQ"
     gene            499..>1227
                     /locus_tag="PKZ52_19655"
     CDS             499..>1227
                     /locus_tag="PKZ52_19655"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNG62046.1"
                     /translation="MYRMFSALIALLLISLPSWVQAGILHEERSLYRNIIVSEADGQR
                     CMRFVLHDKVNHNQSCVFLNDGKKLVFDYTKMVLASLLIKPEPKNILVVGLGGGTLPM
                     VFHQLLPSAQITSVEIDPAVVKAAQRFFAYQENPQVITELLDARVFIKRAGRQGKKWD
                     LVVLDAFNGDYIPEHLMTREFLQEVQAVLNPGGLVAANTFADSRLYHHETATYNAVFS
                     HIHMLSFRRGNRVLFAANDAFSLPA"
BASE COUNT          277 a          278 c          314 g          358 t
ORIGIN      
        1 ggttattaat tttaccggtg gtgtttatcg cccaacctat tatggctgct atttttgatc
       61 gcatagtgga tgctcgctac ggctcgctgg cggcttcatt attgttattt ttattgccgt
      121 ctattgtgat gggaatggta tcgccctatt cggtgcgttt gttaacccaa tcgcgggaat
      181 cgagcggcgc ctcagcgggc gtgctgtatt ttgtctctac cctcggcagc gccttaggca
      241 ctttggccac ctctttttat ttggtggcac tgtgggagat aaatcaaata ttgctggcgg
      301 cggtggctgt tttttgcctg aatggactgc tgttgatagc cgcccatagc cgtatgcctg
      361 tgcagtaaag aaataggcgt tttaataaga gggatctttt gggcgcgcct acggatgtat
      421 tcagcgcgtg tcaccacctt tatccattga tcctggctcg cgagtatcct tgttcactcc
      481 accaactgag aagtaaatat gtatcgcatg ttctctgcgc ttatcgcatt acttttgatt
      541 agcctgcctt catgggtaca ggcgggaatt ctgcacgaag agcgttcgct gtatcgcaat
      601 attattgtca gtgaagcgga cgggcagcgg tgcatgcgtt ttgtattgca cgacaaggta
      661 aaccacaacc aatcctgtgt gtttttaaac gatggaaaaa aactcgtgtt tgattacacc
      721 aaaatggtac tggcatctct attgattaaa cccgagccta aaaacatttt ggtggtgggg
      781 ttagggggcg gcaccttgcc catggtgttt catcaattat tgcccagtgc gcaaattacc
      841 tcggtggaaa ttgacccggc agtggtaaaa gcggcccagc gcttttttgc gtaccaagaa
      901 aacccacagg taataaccga attattagat gcgcgggtat ttattaaacg cgccggacgg
      961 cagggcaaaa aatgggactt ggtggtgctg gatgcattta atggcgacta tattcccgaa
     1021 catttaatga cccgcgaatt tttgcaagaa gtgcaagcgg ttttaaatcc aggcggtttg
     1081 gtggcggcca atacctttgc cgacagccgt ttataccacc acgagacggc cacctataac
     1141 gcggtgttta gccatattca tatgctgagt ttccgccgcg gtaaccgagt gttatttgca
     1201 gcaaatgatg ccttctcact acctgcg
//
LOCUS       DAOQGS010000882         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_93176_length_1226_cov_3.811272, whole genome shotgun sequence.
ACCESSION   DAOQGS010000882 DAOQGS010000000
VERSION     DAOQGS010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93176_length_1226_cov_3.811272"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1226
                     /locus_tag="PKZ52_19660"
     CDS             <1..>1226
                     /locus_tag="PKZ52_19660"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNG62047.1"
                     /translation="VFLFPFEAVIKTRHPGSVMASYNEIDGVPSHANTKLLQTILRKE
                     WGFDGIVVSDYFAIPELASRHHLAADPYGAAIKAFNAGVAIETPSGDTYALLKRAIDE
                     GKITPASLDQAVAGVLKPKFALGLFENPYVDEKKADACVGSEAARKVALRAAEQSIVL
                     LKNDSNTLPLNKKALKSIAVIGPHINETLLGGYSDVPRRTTTILQGIKNYLGPKVVVN
                     SALGVKITEDNWQPGSDSIAAQSLSKERWNRNVITPADPQKNRALIKEAVAVAAQSDV
                     TVVVVGDNEGTSREAWGEEHLGDRTDITLFGEQQELVDALLALGKPTVVVLNNGRPLA
                     IEKIHSTAPAIIEAWYLGQETGTALARVLFGEVNPGGKLPVSIARSVGQLPVYYNYKP
                     TAKRGYAFTDTTPLYP"
BASE COUNT          284 a          334 c          349 g          259 t
ORIGIN      
        1 gtgtttttat tccccttcga ggccgtgatt aaaacccgcc acccaggcag tgtgatggcg
       61 tcctacaacg aaattgacgg cgttccctcc catgccaata ccaagctgtt gcagaccatt
      121 ttgcgcaagg aatggggttt tgacggcata gtggtgagtg attattttgc tattcctgag
      181 ttggcgagcc gccatcacct ggcggctgat ccctatggtg cggccattaa agcctttaac
      241 gccggtgtcg ccattgaaac cccctcaggt gatacctatg ccctgttaaa gcgggcgatc
      301 gacgagggca aaattacccc cgcgagtttg gatcaggcgg tggctggagt gttaaagccc
      361 aagttcgccc tgggcctgtt tgaaaacccc tatgtagacg agaaaaaagc cgatgcctgc
      421 gtgggctctg aggcggcgcg caaggtggcg ctgagggcgg ccgagcaatc catcgtgctg
      481 ttaaaaaacg acagcaatac cctgccgcta aataaaaaag cccttaaatc catcgccgtg
      541 atcggccccc acatcaacga aactctgctg ggcggctaca gcgatgtgcc ccgccgtacc
      601 actactattc tgcagggcat taaaaattat ctggggccaa aagtagtggt taatagcgcc
      661 ttgggtgtga agattaccga ggacaactgg cagccgggca gcgactcaat tgccgctcag
      721 agcctgtcaa aggagcgctg gaataggaat gttattactc ctgcggatcc gcaaaaaaac
      781 cgcgccctca tcaaagaggc ggtggctgtt gccgctcaga gcgatgttac ggtggtagtg
      841 gtgggggata acgaaggcac ctcgcgggag gcctggggtg aggagcactt gggggatcgc
      901 accgacataa ccctatttgg cgagcagcaa gaattggtgg atgcactgct ggccctgggc
      961 aaacccacgg tggtggtgct caataacggc cgccccctgg ccattgaaaa aattcacagc
     1021 acagcccccg ctatcatcga agcctggtat ctaggccaag aaactggcac ggccctggcg
     1081 cgggttttat tcggtgaggt taaccccggc ggtaagttgc cggtgagtat tgcccgttcg
     1141 gtggggcagc tgccggtcta ttacaactac aaacccaccg ccaaacgcgg ttatgccttt
     1201 actgatacca caccgcttta cccctt
//
LOCUS       DAOQGS010000883         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_93600_length_1223_cov_4.094178, whole genome shotgun sequence.
ACCESSION   DAOQGS010000883 DAOQGS010000000
VERSION     DAOQGS010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93600_length_1223_cov_4.094178"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..760)
                     /locus_tag="PKZ52_19665"
     CDS             complement(257..760)
                     /locus_tag="PKZ52_19665"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNG62048.1"
                     /translation="MLRPAEPKDATAIGTIRVAAWQAAYSHFLPEDFLTGLDPNANLD
                     FLRTQLAQQSDDFRVWVAEADPIVVGFCIIGKPRYQAGPSSLEIWAINVSPYYWRKGI
                     GQQLLTQVINYYKHQYAQLTLWCIKGNLPAQRCYEQLGFMPSGQARSTTHLTGHPLHE
                     IEYIKTL"
BASE COUNT          296 a          264 c          315 g          348 t
ORIGIN      
        1 ttataaagac gagcgtaccc agcagctggg ttttgattct taatggtgtg ggcatgatag
       61 ggagctccaa taaagggatc gaaagacacg gttaaaaagg gtttttgcag ttagccctta
      121 tttgcttgcc ctgtgaatac gtaaatacac gtatttgcca aaaagcgtga gctggcgcac
      181 ttattaaccc ggcgacaaaa aagatcgtcc tgatctattt tgccgccggg ttaataagaa
      241 actcgttgca aatagcctaa agggttttga tatattcaat ctcgtgcagc gggtgcccag
      301 tgaggtgagt ggttgaacgc gcttgcccac tgggcataaa acccaattgt tcataacagc
      361 gctgggcggg taggttacct ttgatgcacc agagggttaa ttgtgcatat tgatgtttat
      421 agtaattaat tacctgagtt aataactgct ggcctatacc cttgcgccaa taatagggtg
      481 atacattaat ggcccaaatt tctaagctgc tcgggcctgc ttgatagcgc ggtttgccga
      541 taatacaaaa gcccacaact attgggtctg cttcggcaac ccacactcta aaatcatcac
      601 tttgttgggc caattgggtg cgcaaaaaat ccaaattcgc gttgggatct aagccggtta
      661 aaaaatcctc gggtagaaaa tggctgtacg cggcttgcca ggcggccacg cggatggtgc
      721 caatggcggt ggcgtctttg ggttcggcgg gtcttagcat cttaattatt ccttttttaa
      781 caatttattt ttatctcgtt cccacgctct gcgtgggagc gcaagcttat tttgccttaa
      841 gtttgcgaat cttttccacg caatctcaat atgcgttccc acgcagagcc tgggaacgag
      901 agtagctggc gcaggtgacg tgttcgacgg tgctgggtgt tttgcgggct gcacagtagt
      961 tggcagggca ggttttggcg gtagcctgga ggcgtttaag gactggcggg taaccactgc
     1021 ttttatgtgt gaggcgagac acgcctgcaa acgaccgtct tcgggtgcta ttttaacagg
     1081 ctgtttagtc ggccacattt ttacttctaa aaattctaac ccctgcccaa tggcacttaa
     1141 gtcattctgg ctgcgctgaa tttatgacgc acagcgggat gaatattgac gttaattaat
     1201 cttgccaaat tcctgttgtt aat
//
LOCUS       DAOQGS010000884         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_94624_length_1216_cov_4.589147, whole genome shotgun sequence.
ACCESSION   DAOQGS010000884 DAOQGS010000000
VERSION     DAOQGS010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94624_length_1216_cov_4.589147"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1216
                     /locus_tag="PKZ52_19670"
     CDS             <1..>1216
                     /locus_tag="PKZ52_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62049.1"
                     /translation="DGGTNTVESQLAASRWDLSSTANGVIFNGDETALSYSGANNWVA
                     LAGGDNQLSFANLAVDLQWVINASSNERAAGQVWAAVQPGSSSQFSGFNHITGAKGTD
                     DITLAAAISQINLGEGNNRLTLQTGAKVTRLSAGAGSDEIKSFSAANLWTLLSATEGK
                     LSTPSLDVVGQFTGFDVLTGSGNDRLSATAAANKWFVNGPGSGNLNNAYTFTGFGRLQ
                     GNSGTDTFEIGALGSMGTLEGGEGEDTLTARNSANSWSFTRNDTGVIRATGEQTNYLG
                     FGGIETFTGAAAAADSIRLDPEVTQVKLAAGGQAGDELFVNYRDNLNLTSDGASWLGV
                     SGVKKVTAQPLTANRSAQLTSTASAASRWIINEDNGGDWTVGVGTDTASTTHFSGFDK
                     LQGSATADEFILS"
BASE COUNT          286 a          305 c          369 g          256 t
ORIGIN      
        1 gatggtggca ccaacacggt ggagagccaa ttggcggcta gccgttggga tttatcctcc
       61 actgccaatg gcgttatttt taatggcgat gaaacggcgc tgagctacag cggcgcgaat
      121 aattgggtgg ccctggccgg tggggataat cagttaagtt ttgccaacct tgcggtggat
      181 ttacagtggg tgattaacgc cagtagcaat gagcgcgccg caggccaagt ttgggcggct
      241 gtccaacccg gttcgagcag ccaatttagt gggtttaacc acattactgg cgccaagggt
      301 accgatgata taaccctagc cgcagccatt agccaaatta atttaggcga gggcaataac
      361 cgtctcacgc tacagaccgg agccaaagtc acacgcctta gcgcaggggc aggttcggat
      421 gaaataaaaa gtttttcggc ggccaacctg tggacactgc tcagtgccac cgagggtaaa
      481 ttgagtacgc caagccttga tgtggtgggt cagtttaccg gctttgatgt gttgaccggt
      541 tcgggcaacg accgcttaag cgcaacagct gcagcgaaca aatggttcgt taatggccct
      601 ggcagcggca atctcaataa cgcctatacc tttaccggtt ttggtcgcct gcagggcaat
      661 agcggtacgg atacgtttga gattggtgcg ctgggcagca tgggcactct tgagggcggc
      721 gaaggcgagg acactttaac agcccgtaac agcgcgaata gttggagttt tacacgcaac
      781 gatacaggcg tgatccgggc cactggcgag cagactaact acctaggctt tggcggtatt
      841 gagacgttta ccggcgctgc ggccgctgcg gatagcatca ggctagatcc tgaggtgacc
      901 caggttaaat tggcagctgg gggccaagca ggggatgagt tattcgttaa ttaccgcgac
      961 aacctcaatc tcaccagcga tggcgccagc tggttgggtg tcagcggggt gaaaaaagtg
     1021 acggctcagc cgctcaccgc caaccgcagt gcgcaattaa ccagtactgc aagcgccgcc
     1081 agccgctgga tcatcaacga agacaacggc ggcgattgga cggtgggtgt gggcaccgac
     1141 accgcttcaa ccacgcactt tagcggcttt gataaactgc agggcagtgc cacggcggat
     1201 gaatttatct taagtc
//
LOCUS       DAOQGS010000885         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_95555_length_1210_cov_4.270996, whole genome shotgun sequence.
ACCESSION   DAOQGS010000885 DAOQGS010000000
VERSION     DAOQGS010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95555_length_1210_cov_4.270996"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..697
                     /locus_tag="PKZ52_19675"
     CDS             29..697
                     /locus_tag="PKZ52_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007232886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNG62050.1"
                     /translation="MLEVSNVSHEVSLSDGSRLPLLHQVSLTVHKGESVAIIGASGSG
                     KTTLITLMASLDVPSQGRISIAGQDVGGLDEEARAAVRGRFIAFVFQNFQLLDGLTAL
                     ENVLLPLEVRAVDRAEALACEYLQAVGLGERTGHYPAQLSGGEQQRVALARAFACRAP
                     LLFADEPTGNLDETTGSTIADLLFAFNKHQGTSLVLVTHDLALASRCDKRYRMSAGRL
                     ELAP"
     gene            694..>1210
                     /locus_tag="PKZ52_19680"
     CDS             694..>1210
                     /locus_tag="PKZ52_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62051.1"
                     /translation="MSLAFTLLWRQWRSRNVRLMFISLVVAMATLLNLHLLTARIEHS
                     VARSAKELLAADVRVETSKPLAESVQKLIAPFTERSSRAIEFQSMAYASQADDGQLAQ
                     IKAVEAGYPFYGQVLDEQRQALAGQPEPGQAWVNGKLAERLNVKVGDNLDIGEAQFRV
                     GAIFTAEPDDPQ"
BASE COUNT          261 a          311 c          367 g          271 t
ORIGIN      
        1 ggtaactgcg cgaatgagcg atgaaatcat gttggaagtc tcaaatgtaa gccacgaggt
       61 gagtctgtca gacggtagcc gcttaccttt gttgcatcaa gtttcgctaa ccgtgcataa
      121 aggcgaatcg gtggcgatta tcggcgcttc aggctcgggt aaaaccactt taattaccct
      181 gatggcaagc ttagatgtgc caagccaagg gcgtattagc atcgccgggc aggatgtcgg
      241 cggcttggat gaagaagcgc gagcggcggt acgcggccgg tttattgcct ttgtgtttca
      301 aaactttcag ctgctcgatg gtttaacggc actggaaaat gtgctgttgc ccctggaagt
      361 gcgggcggtg gacagggcag aagcgctggc ctgcgaatat ttacaggcgg tgggtttagg
      421 tgagcgcacc ggccattacc cggcgcaatt atccggcggt gagcagcagc gggtggcctt
      481 ggcgcgagcc tttgcctgcc gggcgccgct gttatttgcc gatgaaccca cgggcaattt
      541 ggatgaaaca accggcagca ccattgccga tcttctcttt gccttcaata aacaccaggg
      601 caccagccta gttttagtaa cccacgattt agccttagcc agccgctgtg ataaacgcta
      661 ccgcatgagc gctggccgct tggagcttgc gccgtgagcc tggcgtttac cctactgtgg
      721 cggcagtggc gcagccgcaa cgtgcgcctg atgtttatta gcctggtggt ggccatggcc
      781 acactgctga atttgcactt attaacagcg cgcattgaac actcggtggc ccgctcggcg
      841 aaagaattat tagccgccga tgtgcgcgta gaaaccagta aacccctagc tgaatctgtg
      901 caaaaactca tcgcgccctt caccgagcgc agcagccggg ctattgaatt tcagtccatg
      961 gcctatgcaa gccaggccga tgatggccag ttggcgcaaa ttaaagccgt tgaagcgggt
     1021 tacccctttt atgggcaagt gctcgacgag cagcgtcagg ctctagcggg ccagcctgaa
     1081 cctgggcagg cctgggtgaa tgggaaactt gccgagcggc tgaatgtaaa ggttggcgat
     1141 aacctggata taggtgaggc gcaatttcgc gtcggggcaa tttttactgc ggagcctgat
     1201 gaccctcagg
//
LOCUS       DAOQGS010000886         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_97265_length_1199_cov_4.301573, whole genome shotgun sequence.
ACCESSION   DAOQGS010000886 DAOQGS010000000
VERSION     DAOQGS010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97265_length_1199_cov_4.301573"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKZ52_19685"
     CDS             complement(<1..583)
                     /locus_tag="PKZ52_19685"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNG62052.1"
                     /translation="MFNPLLILRRIFAITGKEFKELRRDRLTFGMVVMVPLIQLILFG
                     YAINTDVRGLSVGVVDFSQSHFSRAAIASISATGVARFTHSFNSIAEAEAAITAGSVH
                     GVLVLPDDFSRRIEDSLNPSLTQGDRPKRRLGQWLVDGSDTLVASAIKNLRLLPLDEI
                     LGARAKQLEPTFDMAFYFNPEQRTAINIVPGLLA"
     gene            complement(576..>1199)
                     /locus_tag="PKZ52_19690"
     CDS             complement(576..>1199)
                     /locus_tag="PKZ52_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNG62053.1"
                     /translation="VARIYGLNRNAAKTRIEFLLQQYQLAARKTQMAGTLSGGQKQRL
                     ALACAVLHQPRLLFLDEPTSAVDPENRREFWERLFDLCEQGTSILVSTHYMDEAERCH
                     GLAIMDQGELMAQGSPQILMEQMAAHVVEVDGENVRALKNTLLQLSPILSAAQLGTRL
                     RVLICKEINAPADWLRAQLPAQYQVNQVRPSLEDVFVMSTRKARPHV"
BASE COUNT          290 a          307 c          319 g          283 t
ORIGIN      
        1 ccgccagtag gcccggcact atattaatag ctgtgcgctg ctcaggatta aaataaaacg
       61 ccatgtcaaa agtgggttct aattgtttag cgcgtgcgcc cagaatttca tccaggggca
      121 ataaacgcag gtttttaatg gccgaggcga ctaaggtgtc ggagccatcc actaaccatt
      181 ggcccaagcg gcgtttgggg cgatcgccct gggtgagcga tgggttaagg ctatcttcaa
      241 tgcggcggct aaaatcgtct ggcaacacca ataccccatg cacgctacct gcggtaatgg
      301 ccgcttccgc ttcggcgatg gaattaaacg agtgggtaaa acgggccaca ccagtggcgc
      361 tgatactggc aatggcggcg cggctaaaat ggctttggct gaaatccacc acgcccaccg
      421 aaaggccgcg cacatcggta ttaatggcgt agccaaataa aattaattga attaatggca
      481 ccatcaccac catgccgaag gtgagtctat cccggcgcaa ttctttaaat tctttaccgg
      541 taatggcgaa tatgcgccgc aatatcaata ggggattaaa catgggggcg ggccttgcgc
      601 gtgctcatta caaaaacatc ttcaaggctc ggccgcacct ggttgacctg atactgcgcg
      661 ggcagctggg cgcgcaacca atcggcaggt gcgtttatct ctttgcatat taatacccgc
      721 aggcgggtgc ctaattgggc ggcggataaa atggggctta attgcaagag tgtatttttt
      781 agtgcccgca cattctcacc atccacttcc accacatggg ccgccatctg ttccatcagt
      841 atttgggggc tgccttgggc cattaattca ccctggtcca taatggccag gccgtggcag
      901 cgctcggctt cgtccatata atgggtagaa actaaaatgc tggtgccctg ttcgcacagg
      961 tcaaataaac gctcccaaaa ttcccggcga ttttccgggt ccaccgccga ggtgggttcg
     1021 tctaaaaata ataaacgcgg ctgatgtaat acggcacaag cgagggccaa acgctgtttt
     1081 tgcccaccgc ttaaggtgcc tgccatttgg gttttgcggg cagctaattg atattgctgc
     1141 aataaaaatt cgatgcgggt ttttgcggcg ttgcggttta agccataaat acgcgctac
//
LOCUS       DAOQGS010000887         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_99755_length_1183_cov_2.427305, whole genome shotgun sequence.
ACCESSION   DAOQGS010000887 DAOQGS010000000
VERSION     DAOQGS010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99755_length_1183_cov_2.427305"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1183)
                     /locus_tag="PKZ52_19695"
     CDS             complement(<1..>1183)
                     /locus_tag="PKZ52_19695"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNG62054.1"
                     /translation="THLSIRNLTLVDSLDLELAAGLTVLTGETGAGKSILLDAIGLSL
                     GDRADADKIRQGEDKAEVSAQFDLAARPDILAWLEAEDLAQQAECILRRSLSREGRSR
                     AFINDRPVTLTQLRTLGEQLINLHSQHEHQALMDTAHHRWLLDHFGQLQGQLQPVKEA
                     FNRWQQLSQEYQRVCAQADELNARFQLLSYQVEEFDQLAVQEGELEHLELQQKQLTHA
                     DAFNQCCQSVVDICQADKGLRDRLYRAIQLLGQVPIEHKSVQEAQALLQSAQINIEEA
                     SSELMHALDANHHLDGDLPSIERRLSSLYEMARKHRVKPTELVDLHGRLSAELAGRKS
                     GDEQLAGLESAIAAALKHYQSATLALSQARNSVAQDLSEKVNLQLAQLSLGHAEFSIS
                     LN"
BASE COUNT          207 a          335 c          362 g          279 t
ORIGIN      
        1 gtttaggctg atactaaact cggcgtggcc gaggcttaac tgggccaact gcaggtttac
       61 cttttcactt aaatcctggg ccaccgagtt gcgggcttgg cttaatgcga gggttgcgct
      121 ttggtagtgt ttgagggctg ccgctatggc gctctcaagg cctgccaatt gttcatcgcc
      181 acttttacgc ccggccagct cagcgctcaa acgcccgtgc aagtccacca attccgtggg
      241 tttaacccgg tgtttacggg ccatttcgta taggctagag aggcgccgct cgatggaggg
      301 caagtcgccg tccagatggt ggttggcgtc tagcgcgtgc atcagctcgc tgctagcttc
      361 ttcaatatta atttgcgcgc tttgcagcag ggcctgggcc tcctgcacgg atttgtgctc
      421 gatgggcact tgccccaaca gctgaatggc ccggtagagg cgatcccgca ggcccttatc
      481 ggcttggcag atgtccacca cgctctggca gcactggtta aaagcatcgg cgtgggtgag
      541 ttgtttttgc tggagctcca agtgctcaag ttctccctcc tgcaccgcca gctgatcgaa
      601 ctcctccact tgataactga gaagttggaa gcgggcgttg agttcatccg cctgggcgca
      661 cacgcgctga tactcctggc taagctgctg ccagcggtta aaagcctctt taaccggttg
      721 cagctggccc tgcaattggc cgaagtgatc cagcagccag cggtggtggg cggtgtccat
      781 gagagcttgg tgttcgtgtt ggctgtgcag gttaatcaac tgttcgccga gggtgcgcaa
      841 ttgggtcaat gttaccgggc ggtcgttaat aaaggcgcgg gagcggccct cccggctgag
      901 gctgcggcgc aggatacact cggcctgctg ggcaagatcc tccgcttcta accaggcgag
      961 aatatctggc cgggcggcga ggtcgaactg ggcgctcact tccgccttgt cctcgccctg
     1021 gcggattttg tcggcgtcgg ctctatcgcc cagggacagg ccgatggcgt ccagcaaaat
     1081 ggatttacct gcgccggttt cccccgtcag caccgtaagc cccgccgcta attcaaggtc
     1141 gaggctgtcc actagcgtca ggttgcgaat gcttaaatga gtg
//
LOCUS       DAOQGS010000888         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_100018_length_1181_cov_3.497336, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000888 DAOQGS010000000
VERSION     DAOQGS010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100018_length_1181_cov_3.497336"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1181
                     /locus_tag="PKZ52_19700"
     CDS             <1..>1181
                     /locus_tag="PKZ52_19700"
                     /inference="COORDINATES: protein motif:HMM:NF019256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HNG62055.1"
                     /translation="NRGQHISTGPRAQPKYDWTASQEIAIPHAGVLSEPAFLQQYPTT
                     ATNRNRARSRWTYQHFLGVDIENSAARTIDSAALSDNDNPTLNNSACTVCHTRLDPLA
                     GAFQHFGERGTFHDQPRGTNALDDSYRRSKLYKAGDTWYRDMRLPGFEQALTPDPLNS
                     LHQAAELIAQDPRFATGTVKFWWPALFGESLLDDNLSSTQYDAKLTALNHFADFFTAN
                     NFALKPLLVEMLMSDWFRGKAPAAEATESPAIYTGGKRLLTPEELYNKTFDLTGVADT
                     NLLKGFKLSFGGIDSVSATKRQRDLSHIMLRVVERHALSKSCTIVATEFNKPKEERKL
                     FNLVERQTLPSSAYQTVEVFEQTSARDLTWSIPVVAVPGQKINFVATQTKSVQTDAAL
                     DT"
BASE COUNT          307 a          338 c          273 g          263 t
ORIGIN      
        1 aaccgcggcc aacacatttc taccggcccg cgcgcccagc ccaaatacga ttggaccgcc
       61 tcccaggaaa ttgcaattcc ccacgctggt gttttatcgg aaccggcatt tttgcagcaa
      121 tacccaacca cggcgaccaa tcgcaaccgc gcgcgctcac gctggaccta tcaacatttt
      181 ctgggtgtgg atattgaaaa ttctgcagcg cgcactatcg actctgcagc cctgagcgat
      241 aacgacaacc ccacactcaa caactcagcc tgcacggtat gccacacccg tttggatccg
      301 ctggcgggtg cattccagca ttttggcgag cgcggcacat ttcacgatca gccaaggggc
      361 acaaatgcgc tggatgacag ctatcgacgc tccaagttat acaaggcggg tgatacctgg
      421 tatcgcgata tgcgattgcc tggctttgag caggctctaa cgcctgatcc gctcaattct
      481 ttacaccaag ccgccgaatt aattgcgcaa gatcctcgct ttgccacagg caccgttaaa
      541 ttctggtggc ctgcactgtt tggagagtca ctcttggatg acaatcttag ctcgactcaa
      601 tacgacgcta aattaaccgc gctaaatcat tttgctgact ttttcacagc caataacttc
      661 gccctaaaac ccttgttggt tgaaatgctc atgagtgatt ggtttagagg caaggcccct
      721 gcagcggaag ccacagaaag cccagcgatt tataccggcg gtaaacgcct attaacgcct
      781 gaggagctgt acaacaaaac ctttgatctc accggcgttg ccgataccaa tttactcaaa
      841 ggatttaagc tgagttttgg cggcatagat tcggttagtg caaccaaacg ccagcgggat
      901 ttaagccaca ttatgctgcg ggtagttgag cgacacgctc tatccaaaag ctgcaccatc
      961 gtagcgacgg aatttaataa acccaaagaa gagcgcaaat tgtttaacct ggtcgagcgg
     1021 caaacgcttc ccagtagcgc ctaccaaacg gtagaagttt ttgaacaaac cagcgcccgc
     1081 gatctcacct ggtcgattcc ggtcgtagcc gtgcccgggc aaaaaattaa ttttgtcgcc
     1141 acgcaaacca aatcggtaca aaccgatgcc gcactggata c
//
LOCUS       DAOQGS010000889         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_100335_length_1179_cov_4.073843, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000889 DAOQGS010000000
VERSION     DAOQGS010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100335_length_1179_cov_4.073843"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1179
                     /gene="rlmKL"
                     /locus_tag="PKZ52_19705"
     CDS             <1..>1179
                     /gene="rlmKL"
                     /locus_tag="PKZ52_19705"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HNG62056.1"
                     /translation="NFDASAWQQLITEAQEQRAASINKGLPLMLGCDEFSRVLEAATN
                     NIERAGLRDFIQLKRAGIARFEKPSSWEQGLILTNPPYGERLGEVETLKNTYQDLGRV
                     VKAQCTGWTFGVFTGNTELAKELRLRSNKRYKLFNGTIASELILYQVLPPEVATLRED
                     GPTPKQTAEQPLPPLSNGATMLANRLRKNLKQFKSWIEKNSIDAYRLYDADMPEYSAA
                     IDIYGESIHVQEYAAPKSIDPAAAAKRFDEIIAAVCEVFNQRPEQLAIKTRQRNKGKQ
                     QYEKLQNSDGEYFSVQEGLAEYWVNLTSYLDTGLFLDHRPLRRMVAAEAKGKRVLNLF
                     CYTASITVQGILGGAKSSVSVDMSNTYLHWAKKNFELNKIYGDKHQLVQADCFKWLAQ
                     C"
BASE COUNT          330 a          296 c          289 g          264 t
ORIGIN      
        1 aattttgacg ccagcgcctg gcagcaatta attaccgaag cgcaagagca gcgcgcggcc
       61 agtatcaata agggcctgcc cttaatgctc ggctgcgacg aattttcacg ggtattagaa
      121 gccgccacca ataatatcga gcgcgcaggc cttagggatt tcatccaatt aaaacgcgct
      181 ggtatcgccc gctttgaaaa accctccagc tgggagcagg gtttgatttt aactaacccg
      241 ccttatggtg aacgcctagg ggaagtggaa actctaaaaa atacctacca ggatttaggc
      301 cgcgtggtaa aagcccagtg caccggctgg acctttggag tatttaccgg taatacggaa
      361 ctcgccaaag agctgcgcct gcgctccaat aaacgctata aattatttaa tggcaccatc
      421 gccagtgaat taattttata tcaagtattg ccgccggaag tggctaccct gcgcgaggac
      481 gggccgacgc ctaaacaaac tgccgagcag ccgctgccgc ccttaagtaa tggcgccacc
      541 atgctcgcca atcgtttgcg caagaattta aaacagttta aaagctggat tgaaaaaaac
      601 agcatagacg cctaccgcct gtacgatgcc gatatgcccg aatactccgc cgccattgat
      661 atttacggcg agtccattca cgtacaggaa tacgccgccc ccaaatccat tgaccctgcc
      721 gccgccgcta aacgctttga tgaaattata gccgccgtgt gtgaggtatt taatcagcgc
      781 ccagaacagc tcgccatcaa aactcgccag cgcaataaag gcaaacagca gtatgaaaaa
      841 ctgcaaaata gcgatggtga atatttttcc gtgcaggaag gcttggcgga atactgggtt
      901 aatttgacca gttatttaga cacaggccta tttttagatc ataggccact gcgccgtatg
      961 gtggcggctg aggccaaagg caagcgggta ttaaacctct tttgttatac cgcgagcatc
     1021 acggtgcaag ggattttagg cggcgctaag agtagtgtca gtgtggatat gtccaacacc
     1081 tatttacatt gggcgaaaaa aaactttgag ctgaataaaa tctacggcga taaacaccaa
     1141 ctggtgcagg cggattgttt taaatggctc gcccagtgc
//
LOCUS       DAOQGS010000890         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_101007_length_1175_cov_3.542857, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000890 DAOQGS010000000
VERSION     DAOQGS010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101007_length_1175_cov_3.542857"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1049
                     /locus_tag="PKZ52_19710"
     CDS             153..1049
                     /locus_tag="PKZ52_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNG62057.1"
                     /translation="MQLFSFPFTAMGSPCELQLYGADAGHLRTLANLAMAEACRLEKR
                     YSRYRPDSLLSQINRVAAAGGEIRVDEETASLLNYAQTCYQQSDGLFDITSGLLREAW
                     DFKSGRLPQQSQIDALLARIGWHQVFWQPPTLSFPPGMQLDFGGIVKEYAADRLASLC
                     WNAGCQQGFINLGGDIRVLGPHPDGSPWRIGVKHPRSEGAAPLRIIELSQGGIASSGD
                     YERGIEINGKRYGHILNPKTGWPVQHLAAVTVVADLCVLAGSAATIGMLKEQAGPEWL
                     ANLGLDYVWVDTAGGTGASLAQ"
BASE COUNT          253 a          348 c          332 g          242 t
ORIGIN      
        1 ggcctgggcc tcagtgcaca tttcagctct ggcccctttg cgggtatgaa cctagggctg
       61 gaatggctgc agccggtgga tgacaaaccg gccggctacc agcttgaacg ccagggcagc
      121 ctggtggtga acctgcgcaa acacctgtaa ccgtgcagct atttagcttc cccttcaccg
      181 ctatgggctc cccctgtgag ctgcaactct acggggcgga tgccgggcac ctgcgcaccc
      241 ttgcaaacct agccatggcc gaagcttgcc gactggaaaa gcgctactcc cgctaccggc
      301 cagacagcct gttaagccag attaatcggg tggcagcagc aggtggtgaa atccgggtgg
      361 acgaggagac cgccagcctg ctcaattacg cccaaacctg ttaccagcag agcgatgggt
      421 tattcgatat tacctccggc ctactgcgcg aagcctggga ttttaaatcc ggccgcctgc
      481 cccagcagag ccagatcgac gcattacttg cgcgcatcgg ctggcaccag gtgttctggc
      541 agccaccaac acttagcttt ccgcctggca tgcagttgga ttttggcggc atagtcaaag
      601 aatacgctgc cgatcgcctg gccagcctgt gttggaacgc cggttgccaa cagggcttta
      661 ttaacctcgg cggcgatatc cgcgtgctcg gcccccaccc cgacggcagc ccctggcgca
      721 taggcgttaa acacccacgc agcgagggcg ctgctccctt gcggattatt gaactaagcc
      781 agggcggcat agccagctcc ggcgattacg agcgcggtat tgaaattaac ggcaagcgct
      841 acggccatat cctcaacccc aaaaccggtt ggccagtgca gcatcttgcg gcagtgacgg
      901 tagtggcgga cttatgtgta ttggcgggca gcgccgctac catcggcatg ttaaaagaac
      961 aagcggggcc tgagtggctg gctaacctgg ggcttgacta tgtgtgggtg gatacagcgg
     1021 ggggcacagg ggcttcgctc gctcaatagc agcagcaccc ttttattaat ctgtctcgaa
     1081 ttgattaact tgaattgaat ttattactac ctagacaacc gcaatgaata cacatcattt
     1141 cagacacgcc gtaaatacat ccctgtaggc tcgtc
//
LOCUS       DAOQGS010000891         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_101337_length_1173_cov_4.074240, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000891 DAOQGS010000000
VERSION     DAOQGS010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101337_length_1173_cov_4.074240"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKZ52_19715"
     CDS             complement(<1..687)
                     /locus_tag="PKZ52_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNG62058.1"
                     /translation="MTPNSADTAPHLLLHLEPVEARRLANLSGQYDCHLKQIEQRLSI
                     SIHNRGNAFVLEGDAERLQIAAQVLEQLYAETAGDKLIAPDDVHLALQEHGAEAPMAA
                     EPTQPPEVYSAIRTKKGNVKARGANQQSYVAAIRTHDINFGIGPAGTGKTYLAVACAV
                     ESLLKDDVERILLVRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALYEMLGVEVVGKL
                     IERQVIEIAPL"
     gene            complement(824..>1173)
                     /locus_tag="PKZ52_19720"
     CDS             complement(824..>1173)
                     /locus_tag="PKZ52_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNG62059.1"
                     /translation="SFSFIYSPRPGTPAADLPDDTSEEVKKQRLAILQQRITQQAQAI
                     SQGMIGNIERVLVTGYAKKDPGQLSGRTENNRVVNFRCDQAQLIGKFADILIEEALPN
                     SLRGSLISSELDS"
BASE COUNT          235 a          302 c          368 g          268 t
ORIGIN      
        1 gaggggggcg atttcaatca cctggcgctc aatgagttta cccactacct caaccccgag
       61 catctcgtac agggcgtcgt agaggggccg caggtaaggg tccacttttt gcgataaatc
      121 gccgggtaaa aaaccgagtt tttcgccggc ctccaccgcc gggcgcacca gcaaaatgcg
      181 ctcaacgtcg tcttttagca gggattccac cgcgcaggcg acggctaaat aggttttgcc
      241 ggtaccggca gggcctatac caaagttaat gtcgtgggtg cgaatcgccg ccacatagga
      301 ctgttggttg gcgccacggg cttttacatt gccctttttg gtgcggatgg cggaataaac
      361 ttcggggggc tgagtgggct cggcggccat gggggcctcg gcaccgtgtt cctgtagggc
      421 caggtgcaca tcatccggtg cgatgagctt atctccggcg gtttcggcgt agagctgttc
      481 aagcacttgg gcggcgatct gtaagcgctc ggcatcgccc tctagaacaa aggcattgcc
      541 gcggttgtga atgctgatgg agaggcgctg ttcaatttgt ttaaggtgac agtcgtattg
      601 cccgcttaaa ttggccaggc ggcgcgcttc aaccggttcc aggtggagca acaggtgcgg
      661 cgcggtgtcg gccgagttgg gagtcaactc agccgcaggg gtttgtgtat tcaagaatta
      721 atggctcatg aacaaaagag cgctaagact aactcaatca gggccgggag aaaacctccc
      781 ggcgcggtga ctgtgcagtg tgccacgctc aattcggcgc gaattaactg tccagctcag
      841 agctgatcag gctgccgcgc agggagttgg gcagggcttc ttcaatcaga atatcggcga
      901 atttgccgat tagctgggct tggtcgcagc ggaaatttac cacccggtta ttctcggtgc
      961 ggccacttaa ttggcctggg tcttttttcg cataacccgt caccaatacg cgttcgatat
     1021 taccgatcat cccctggcta atagcttggg cctgctgggt gatgcgctgc tgcaaaatcg
     1081 ccaggcgctg ttttttgact tcctcgctgg tgtcatcggg caggtcagcg gcgggggtgc
     1141 cggggcgggg gctataaata aagctaaaag agg
//
LOCUS       DAOQGS010000892         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_102635_length_1165_cov_4.421622, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000892 DAOQGS010000000
VERSION     DAOQGS010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102635_length_1165_cov_4.421622"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..357)
                     /locus_tag="PKZ52_19725"
     CDS             complement(61..357)
                     /locus_tag="PKZ52_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62060.1"
                     /translation="MMKILVILAGLLGSALIAGCGSGKSGGSSNNATSSSSSSSSSSS
                     SSSSSSGAISAAQTYLGSNNLPPDSGIAAAAVLDAEDFAPNATASGLEGELPPQ"
     gene            complement(357..>1165)
                     /locus_tag="PKZ52_19730"
     CDS             complement(357..>1165)
                     /locus_tag="PKZ52_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62061.1"
                     /translation="FALSISALVYAGNSLAHVGVANNTLPISAAGQAAFTVANTSSEI
                     VFIVGHGCNGEAEPKPPANLDTTKIDITVRAAIVTATGAASVRPSHQGEFGNVTRKVN
                     EDGSIKFTWTKVAAPTADDDQLYKVSIRLKTPAATSASDFAIKKYQFLATQTCAVPGA
                     ADYVLDWGSANSPWLLSFPEKRKGFNKYTLDATTLGDFAVTGSNTKAAKLKSYFGDAA
                     IVWVGKRAYSPNAITSDKVDQLAKKDLTYSNLGTETGVSLSADETIWVRY"
BASE COUNT          235 a          289 c          348 g          293 t
ORIGIN      
        1 attcgcctag ccagagcttc gccgcctaca tttttcagag gttccctagg gtaagtaaat
       61 ttattgcggc ggcaattcgc cttccagccc agaggccgtt gcgttcgggg caaaatcttc
      121 tgcatccaaa acggccgcag cggcgatgcc cgaatccggc ggtagattat tactgcccaa
      181 gtaggtttgg gctgcgctga tggcgccgga ggatgagctg ctgctggagc tgcttgatga
      241 gctactggac gaactgctgg tagcgttatt actggagcca ccggatttgc cactgccgca
      301 gccagcaatt aatgcactgc ccagcaggcc agctaagatc actagtattt tcatcattaa
      361 tatctcaccc aaatggtttc atccgcgctg agggaaacac cagtttcagt gccgaggttg
      421 ctataggtga gatctttttt ggccagttgg tcaactttat cgctggtgat ggcgttaggg
      481 ctgtaggcgc gtttgcccac ccacactatg gcggcatcgc caaagtaact ttttaatttg
      541 gccgctttgg tgttgctgcc ggtcacggcg aaatcgccca gggttgtggc atccagggtg
      601 tatttattaa agcctttgcg cttttcaggg aaggagagca accagggcga attggcgctg
      661 ccccagtcca atacgtagtc tgcagcccct ggcacggcac aggtttgggt ggcgaggaat
      721 tgatattttt taatggcgaa atcgctggcg gaagtagccg ctggggtttt taagcggatg
      781 ctgactttgt agagctggtc gtcatccgcc gtgggggcag ctactttggt ccaggtgaat
      841 ttaatggagc cgtcttcatt cactttgcgg gttacattgc caaattcacc ctggtggctg
      901 gggcgcacag atgccgcccc ggttgccgta actatggccg cacgcactgt gatgtcgatt
      961 ttggtggtat ccaggttggc cggtggcttg ggttctgcct cgccgttaca gccgtggccc
     1021 acaataaaaa caatttcgct actggtattg gcaacggtga atgccgcttg cccggcggct
     1081 gaaatgggca gggtgttatt ggcgacgccc acatgggcga ggctgttgcc cgcatagacg
     1141 agggctgaga tagacaaagc aaagg
//
LOCUS       DAOQGS010000893         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_104137_length_1156_cov_4.358765, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000893 DAOQGS010000000
VERSION     DAOQGS010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104137_length_1156_cov_4.358765"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKZ52_19735"
     CDS             complement(<1..206)
                     /locus_tag="PKZ52_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62062.1"
                     /translation="MIIALVIGLTLALVLGPLLMLKPSSGLQKIAQLRQKAAQMGLLV
                     QAHHHPAITAEGAWMFYLLPWPSFA"
     gene            complement(211..>1156)
                     /locus_tag="PKZ52_19740"
     CDS             complement(211..>1156)
                     /locus_tag="PKZ52_19740"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNG62063.1"
                     /translation="RLLQAAPDLVTLQPFYIPIYSSSKRGNTTIATGLSLYQLLGGGT
                     FRFLNRQQRAALPINKPNLTGLWQYWDCQTHDAALTQRVVSAAQKLGCNLQLDTYAKA
                     IEILAPHAFHLSLNNGTEVHSRCLINAAGPWVNEVAALNTQLPQLNIQWVQGSHLVID
                     RPALNGCYYLESPIDGRPFFVLPWQGKQLIGTTETPINNPSTAAITPAERDYLITSYQ
                     HFFSGPALTPADIITTFCGVRVLPASTSAPQLINRAKRDTQFVQTPDTPGYLALYGGK
                     LTSFYATALKGLQLLAPVLTEANRHLTFTSTTITQPKF"
BASE COUNT          277 a          229 c          352 g          298 t
ORIGIN      
        1 gcgaagcttg gccagggcag caggtaaaac atccaggcac cttcagcggt gatggctgga
       61 tgatggtgcg cttgtactaa caaccccatt tgtgcagctt tttgccgcag ctgggcgatt
      121 ttttgcagtc cgctactggg tttgagcatt aagagcgggc ctagcactag ggcaagggtg
      181 aggcctataa caagtgcaat gatcatgggg ttaaaacttc ggttgtgtga tggtggtgga
      241 ggtgaatgtt aggtggcggt tcgcctcggt caatacgggg gcgagtagct gcaaaccctt
      301 tagggcggtg gcgtagaagg aggtgagttt gccgccatac agggctaaat agccaggggt
      361 atcgggcgtt tgcacaaatt gtgtgtcccg tttagcgcgg ttaattaatt ggggtgcgga
      421 tgtactggca ggcaaaacgc gtacaccaca aaaggtagta attatgtcgg cgggggtaag
      481 tgctgggccg ctaaaaaagt gctgataact ggtaattaaa tagtcgcgct cggcgggggt
      541 tatcgcggcg gtactgggat tattaatggg tgtttcagtg gtgccgatta attgtttgcc
      601 ctgccagggc aatacaaaaa aaggccgccc atcaatggga gactctaaat aataacagcc
      661 atttaatgcc gggcggtcaa tcacgaggtg gctgccttgc acccattgaa tatttaattg
      721 cggcaattgt gtattgaggg cggctacttc atttacccag gggcctgcgg cattaattaa
      781 acacctgctg tgtacctctg tgccgttatt aaggctgagg tggaatgcat ggggggcaag
      841 aatttcaatg gcttttgcat aggtatctag ttgtagattg cagccaagtt tttgggctgc
      901 agatacgacc cgttgtgtaa gggcagcatc atgtgtttgg caatcccagt attgccacag
      961 gccggttaaa ttcggcttat ttatcggtaa agctgctcgc tgttggcgat ttaaaaaacg
     1021 aaatgtgccg ccacccaata attgatagag gcttaaccca gtggcaatag tggtattacc
     1081 gcgtttgctg gatgaataaa tggggatgta aaacggttgt agtgtcacta aatccggcgc
     1141 ggcctgcaac aggcgt
//
LOCUS       DAOQGS010000894         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_104970_length_1151_cov_3.855839, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000894 DAOQGS010000000
VERSION     DAOQGS010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104970_length_1151_cov_3.855839"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKZ52_19745"
     CDS             <1..526
                     /locus_tag="PKZ52_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNG62064.1"
                     /translation="LVVALNKWDGLEEDHKSYVKTELERRLRFVEFADMHFISALHGT
                     GVGNLYQSIERSYQAATEKFSTNFLTRILERAVTEHQPPLVHGRRIKLRFAHTGGHNP
                     PIIVIHGNQTEEIPNHYVRYLEKYFRTALDLHGTPIRFEFKTSDNPYAGKRNKLTKRQ
                     IEKKRRLMKHTRKK"
     gene            553..>1151
                     /locus_tag="PKZ52_19750"
     CDS             553..>1151
                     /locus_tag="PKZ52_19750"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNG62065.1"
                     /translation="MLLQLLPFLPYLILAGVAAGILAGLFGIGGGAVLVPVLFTVLTT
                     LNVPASDAMAMALATSLACIVPTAVSSSYSHYKKNHVQVIILKRWWLGILVGTLLGAQ
                     VINYWRSPYLMLYFSVFLIFVAINQVRPKKTVTRQLPAKPWHYLASFVQGFLSVLAGV
                     GGGTIGVPILLAMGLSIHQAIGTSAALGLFISVFTSAVML"
BASE COUNT          266 a          296 c          281 g          308 t
ORIGIN      
        1 gctggtggta gcgcttaata aatgggacgg cctggaagaa gaccataaaa gttatgtaaa
       61 aaccgagcta gagcggcgcc tgaggtttgt ggaatttgcc gatatgcatt ttatctccgc
      121 cctgcacggc acgggggtgg gcaatttgta tcagtccatc gagcgctctt atcaggccgc
      181 cacggaaaaa ttctccacca attttttaac gcgcatactc gagcgcgccg taaccgaaca
      241 tcagccaccc ctagtgcatg gccgccgcat caaactgcgt tttgcccaca ccggcggcca
      301 caacccgccc attatcgtca ttcacggcaa ccagactgaa gaaatcccca accattacgt
      361 gcgttatttg gaaaaatatt tccgcactgc gctggattta cacggcacgc cgattcgttt
      421 tgaatttaaa accagcgata acccctacgc gggcaaacgc aataaattaa ccaaacggca
      481 aatcgagaaa aaacgccgtt tgatgaaaca cacccgtaaa aaatagcgcc cagttaaatt
      541 caggcgctgg ttatgctgct gcaattgctg ccttttttgc cctatttaat cctcgccgga
      601 gttgcggcgg gtattttggc tggcttgttt ggtataggcg gtggagcagt tttagtaccg
      661 gttttattta cggtgctgac cacacttaat gtgcctgcca gcgacgccat ggccatggct
      721 ttggctacct cgctagcctg tattgtgccc accgccgttt cctccagtta cagccactat
      781 aaaaaaaatc acgtgcaagt cattattctt aagcgctggt ggctggggat tttagtgggc
      841 acgctgcttg gcgcccaggt gattaattac tggcgcagcc cttatttaat gctgtatttc
      901 tcagtgtttt taatttttgt ggccattaac caagtgcgcc ccaaaaaaac cgttacgcgg
      961 cagctgcccg ccaaaccttg gcattatctt gcgtccttcg tgcaggggtt tttatcggtg
     1021 ttggccggtg tgggtggcgg taccataggc gtgcccattt tattggcgat gggtttatcc
     1081 attcaccaag ccataggcac cagcgcagct ttgggtttat ttatttcggt atttaccagt
     1141 gcggtaatgc t
//
LOCUS       DAOQGS010000895         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_107294_length_1138_cov_3.436750, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000895 DAOQGS010000000
VERSION     DAOQGS010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107294_length_1138_cov_3.436750"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1138)
                     /locus_tag="PKZ52_19755"
     CDS             complement(<1..>1138)
                     /locus_tag="PKZ52_19755"
                     /inference="COORDINATES: protein motif:HMM:NF018454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNG62066.1"
                     /translation="PAKVSEQVKDLYFADYIRTWNGFYQSLKLIGLGDVRQAEQMLAA
                     LIDPVYSPMVTALQVGAENTTLTSSLLNDLADSKLKGKKGEAAADVVDKVLDDTPVDK
                     YFKDINSLTKAPKLGQPPIGSALEKVRDLHTFVAEIALAPDPTQKAYEVSKARAQNGG
                     SNPTTALRAYASGTQEPLRGWLESLAIGNSNVISSGGKGFVGSEWRAAVYKPYAQLIM
                     GKYPLNPKAQEETAVLDFTNFFKTGGVLDAFFVANVAPFVDARDPTRVKSVDGGGATF
                     SGDFLRQMQNAQLIRGMFFQDGPDALSLGFDFKPFEFSEKEARFSLEFGNERLTYNHG
                     PKFSSTVKWSAADDIKQLRINFEDVNGKQHEKIYTGQWALLRLFD"
BASE COUNT          282 a          300 c          270 g          286 t
ORIGIN      
        1 cgtcgaacaa acgcaacagc gcccactggc cggtataaat tttctcgtgt tgtttgccat
       61 tcacatcttc aaaattaatg cgcagctgtt tgatatcatc cgccgccgac cattttacag
      121 tggaggaaaa tttaggcccg tggttgtagg ttaagcgctc attgccaaat tccagactaa
      181 agcgcgcttc tttttcgcta aattcaaagg gtttaaaatc aaagcccagg gataaagcat
      241 ccggcccatc ttgaaaaaac ataccgcgaa ttaactgggc attttgcatc tgccgcagaa
      301 aatccccaga aaaggtagct ccgcccccat ccacggattt aacccgcgtg ggatcgcggg
      361 catccacaaa gggcgccacg ttggccacaa aaaaggcatc caaaacaccg cccgttttaa
      421 agaaattcgt aaaatccaac acggcggtct cttcctgggc tttagggttg agcggatatt
      481 tacccataat taattgcgcg tatggcttat aaacggcggc gcgccactcc gagcccacaa
      541 aacccttgcc gcctgaggaa ataacattgg aattgccaat ggctaaactt tccaaccaac
      601 cgcgcagtgg ctcctgggtg ccggaggcat aggcgcgcag tgcggtggtg gggttactgc
      661 cgccgttttg tgcgcgcgct ttggatacct cataggcttt ttgcgtcggg tccggcgcca
      721 gggcaatttc cgccacaaag gtgtgtaaat cgcgcacttt ttccagagcg ctgccaatgg
      781 gtggctggcc taatttaggt gctttggtga gtgaattaat atctttaaaa tatttatcca
      841 ccggggtgtc atccagcacc ttatccacca catccgcagc cgcctcacct tttttgccct
      901 ttaacttact atcggctaaa tcattcaata ggctggaggt gagggtggta ttttccgcac
      961 ctacctgcaa ggccgtcacc ataggtgaat acactgggtc aatgagcgcg gccaacattt
     1021 gttccgcctg acgcacatcc cctaagccaa ttaattttaa ggattgataa aagccgttcc
     1081 aggttcttat atagtcggca aaatagaggt ctttgacttg ttcgctgacc ttagcggg
//
LOCUS       DAOQGS010000896         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_108220_length_1133_cov_3.524119, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000896 DAOQGS010000000
VERSION     DAOQGS010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108220_length_1133_cov_3.524119"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1133
                     /gene="ccmI"
                     /locus_tag="PKZ52_19760"
     CDS             <1..>1133
                     /gene="ccmI"
                     /locus_tag="PKZ52_19760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNG62067.1"
                     /translation="GNSMTMAVFAGLVLLACGFIFWPLLRRSTWQAKLQADTEQDATQ
                     AVLYQEHLTDLEKSLALGEVTQEQFEQLKIELQRTMIAETQPVAAPAARNPGGVWALL
                     AIAVAVPLGAWGFYHHLGAKEDWEIYQLLQTQRNLPDGDASYEKITQELIDKSRARVE
                     EKPKSPQLIYMLASFAMSNQDLAQAETYYRKLLALEPRSPQILAELAQVLFVKSQNVV
                     TAEVREMVKAALSIAPNIPTALSLAGIDEFQQGQFQKAIDYWSRALPMIDPESQGYKA
                     LAGGIEKAKVSLAAAGGTSAPEPAKASGAGPSIEVSVSLGSNASVSPGDTVFVYARAW
                     QGAKMPLAIAKFPVGELPKTVRLDKSMAMAPGMDLTSAPQLEVV"
BASE COUNT          255 a          325 c          326 g          227 t
ORIGIN      
        1 ggtaactcta tgacgatggc agtatttgcg gggctggtgc ttttagcctg cgggtttatt
       61 ttctggcccc tactgcgccg ctccacctgg caggcgaaat tgcaggccga cacggagcag
      121 gatgctaccc aggcagtgtt gtatcaggag catctcaccg acttggaaaa atccctcgct
      181 ctgggggagg tgacccaaga gcaatttgag caattaaaaa ttgaattaca gcgcaccatg
      241 atcgccgaaa ctcagccggt cgcggcgccc gctgcgcgca atcccggcgg cgtatgggcg
      301 ttacttgcca tagccgttgc cgtccccttg ggggcttggg ggttttacca tcatctaggt
      361 gccaaggagg actgggagat ctaccaacta ctgcaaaccc agcgcaacct gccggacggt
      421 gatgctagct atgaaaaaat cacccaggag cttattgata agtcccgcgc acgggttgag
      481 gagaagccta aaagccctca attaatttat atgctggcca gttttgccat gagcaaccag
      541 gacctagccc aggcggaaac ctactaccgc aaactgctgg ccctagagcc gcgctctccg
      601 caaatactgg cggagttggc ccaggtgtta tttgttaaat cgcagaatgt ggttaccgct
      661 gaagtgcgcg agatggtgaa agcagccctg agcattgcgc cgaatattcc cactgcccta
      721 agccttgcgg gtattgacga gtttcagcag gggcaatttc aaaaagccat cgactactgg
      781 tcccgcgctc tgcccatgat cgacccggaa tcccagggct acaaagcctt ggccggcggt
      841 atcgaaaaag ccaaagtcag tctcgccgcc gccggtggca cctcagcgcc tgagcctgcc
      901 aaggccagcg gcgcaggccc cagcattgag gtgagtgtga gcctgggtag caatgccagc
      961 gtcagccccg gcgacacagt attcgtttat gcccgcgcct ggcagggcgc caaaatgcct
     1021 ctggccatag ccaagttccc ggtgggcgaa ttgcccaaaa cggttcgcct ggacaaatcc
     1081 atggccatgg cgccggggat ggacttaacc agtgccccgc agttagaagt ggt
//
LOCUS       DAOQGS010000897         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_108535_length_1131_cov_4.327138, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000897 DAOQGS010000000
VERSION     DAOQGS010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108535_length_1131_cov_4.327138"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PKZ52_19765"
     CDS             complement(<1..834)
                     /locus_tag="PKZ52_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNG62068.1"
                     /translation="MAKKVNSYPEDEQPFKLVCDYQPAGDQPQAIKQLVAGIDAGLAH
                     QTLLGVTGSGKTFTIANVIEKIQRPTLIMAHNKTLAAQLYGEFKEFFPHNAVEYFVSY
                     YDYYQPEAYVPSSDTFIEKDSAVNEHIEQMRLSATKALMERKDAIVVATVSAIYGLGD
                     PEAYLKMVVHLVRGDKIDQRSILRRLAELQYTRNDLEFFRANYRVRGDVIDIFPADSE
                     SIAVRVELFDDVIEKITLFDPLTGEQKGKVARVTIYPKSHYVTPRETIVGAIESIKVE
                     LA"
     gene            complement(838..>1131)
                     /locus_tag="PKZ52_19770"
     CDS             complement(838..>1131)
                     /locus_tag="PKZ52_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanine(2445)-N(2))/(guanine(2069)-N(7))-
                     methyltransferase"
                     /protein_id="HNG62069.1"
                     /translation="QCREGFDLIILDPPSFSNSKRMEDVLDVQEDHVGLISRCMELLN
                     PGGTLYFSTNLRSFKLDAEGLSAFKVADITAASIDPDFARNPKIHQCFRISEA"
BASE COUNT          283 a          272 c          271 g          305 t
ORIGIN      
        1 ggcaagctcc actttaatgc tttcaatggc acccactatg gtttcccgtg gagttacgta
       61 gtggctttta gggtaaatgg tgacgcgggc aaccttgcct ttttgctcgc cagtaagcgg
      121 gtcaaacagg gttatttttt cgatgacatc gtcgaataat tccacccgca cagcgatgga
      181 ttctgaatcc gcagggaaaa tatcgatcac atcgccgcgc acgcggtaat tggcgcggaa
      241 aaattctaag tcgttgcggg tgtattgcaa ttccgccaag cggcgcaaaa tactgcgctg
      301 gtcaatttta tccccgcgca ccaagtgcac taccattttt aaataggctt cagggtcacc
      361 taaaccgtaa atagcggata ctgtcgccac tactatagcg tctttgcgct ccattaaggc
      421 tttagtggcg gacaggcgca tctgctcaat atgttcgttt accgccgagt ctttttcgat
      481 aaacgtatcc gatgagggca cataggcttc cggctggtaa taatcataat aggagacaaa
      541 atattccacc gcgttatggg ggaaaaactc cttaaattcc ccatacagct gggcggcgag
      601 ggttttattg tgggccataa ttaaggttgg ccgctggatt ttttcaatta cattggcgat
      661 ggtaaaggtt ttacccgaac cggttacccc caataaagtt tgatgggcaa ggcccgcatc
      721 aatccccgcc accaattgtt taatcgcctg tggttgatcc ccagccggtt gatagtcgca
      781 caccaattta aaaggctgtt catcctcggg gtatgaattg acttttttgg ccataattta
      841 tgcctcgctg atgcggaaac actggtgaat tttggggttg cgggcgaaat ctgggtcgat
      901 actggccgcc gttatatctg caactttaaa tgcgcttaaa ccttcagcgt ccagtttaaa
      961 actgcgcaaa ttagtggaga aatacaaggt gccgccaggg tttaataatt ccatacagcg
     1021 gctgatgagg cccacatggt cctcctgcac atctaatacg tcttccatac gtttagagtt
     1081 ggaaaaactc ggcgggtcca gaataattaa atcaaagccc tccctgcact g
//
LOCUS       DAOQGS010000898         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_114148_length_1101_cov_7.685468, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000898 DAOQGS010000000
VERSION     DAOQGS010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114148_length_1101_cov_7.685468"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(486..>1101)
                     /locus_tag="PKZ52_19775"
     CDS             complement(486..>1101)
                     /locus_tag="PKZ52_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNG62070.1"
                     /translation="IRAVTLFVCCGPLLAWAEKPVLLVLGDSLSAAYNIDPARSWPQL
                     LQARLPEYQLVNRSISGATTAQGLALLPDSLKHHKPAIVLLELGANDGLQGKPVGLIE
                     DNLNQLILQAKSQGSQVLLIGIRLPPNLGKRYTESFYALYPRLAQRHQVPWVPFLLER
                     VATDNNLMQADGLHPKAEAQEQVLANIWPHLAPLVVAKDVKAAH"
BASE COUNT          252 a          277 c          295 g          277 t
ORIGIN      
        1 tacttccgtg taggctcgtc aaaaaggtcc ctctttttga cggtctgcac taacctgtag
       61 tcaccactgc tacaccgcga atcgattatg ccggatggct aaatcctcct gaatttgccg
      121 cccgcgcgga agaaaggagt aaagtgtgct gaatagttac ggaaaaattt aactaagcgg
      181 catggctgat gcgcggtgta gcagcggtgg ctacagactg tttcagaccg tcaaaaagag
      241 ggatcttttt gacgagccta cacggatgta ttcacggcgt gtctgcaaca gtctgtagtc
      301 attgctgcga tttttaggtt aaagggttcg acttgccgac aggcgcaaaa atgtgtggat
      361 tttcgtgttt ttgcgcggct cgcagcagct tgtacccgcg atgcggcata tttgccgcca
      421 tcattaactc caggtaaaaa tccgtctggt taattcctgc tgctaattat ttcagccagc
      481 ataatttagt gcgccgcttt cacatccttc gccactacca gtggcgccaa atggggccag
      541 atattcgcca gcacttgctc ttgggcttca gccttagggt gcaaaccatc ggcctgcatt
      601 aaattgttat cggtggctac cctctccaat aaaaacggca cccaaggcac ctggtggcgc
      661 tgggctaaac gcgggtagag cgcgtaaaaa ctttcggtgt agcgtttgcc caagttgggc
      721 ggtaggcgaa taccaattaa taacacctgg ctgccctggc ttttcgcctg cagaattaat
      781 tgattgaggt tgtcttcgat taatcctacg ggtttgcctt gtaaaccgtc gttggccccc
      841 aactcaagca gaactatggc cggtttatgg tgttttagac tgtccggcaa cagggctagg
      901 ccctgggcgg tggtcgcgcc gctgatggag cgattgacca gctgatactc gggcaagcgc
      961 gcctgtaaaa gctgtggcca gctgcgggca gggtctatgt tataggccgc gctcaggcta
     1021 tcgcccagca ctaagagtac gggtttttcc gcccaggcca ataaagggcc acaacataca
     1081 aataaggtaa ctgcgcgaat g
//
LOCUS       DAOQGS010000899         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_114572_length_1099_cov_3.847701, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000899 DAOQGS010000000
VERSION     DAOQGS010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114572_length_1099_cov_3.847701"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1099
                     /locus_tag="PKZ52_19780"
     CDS             <1..>1099
                     /locus_tag="PKZ52_19780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNG62071.1"
                     /translation="LGLWRLPTMQRRMREYVALYEQLVANPSLALPDIELILPEDLSL
                     IRASWQADIRPYPLQTVSLHGLIEAAVDRYPEHTALVVGEQTLSYRQLDEMANRLAHF
                     LIQQGVGPEQLVPIMLERSFEMVIAINAVLKAGAAYLPLDPEHPADRIEYIVHEAGAR
                     WVLTTEAFLAKLPSDVACLTMEDAPQRLAELPVSRPRVAVQPEHLAYVIYTSGSTGKP
                     KGVMNEHQAVCNHMLWMQEQFALTPADKVLQKTPYTFDVSLWELFLPLMCGAQLVMAK
                     PGGHKEAGYLVELIQQQGITHTHFVTSMGYLFLQEADQTRCPSLKCVLSSGEAVTKDF
                     EQRFSHAFPDVELWNLYGPTEAAIHITHWQCA"
BASE COUNT          227 a          343 c          310 g          219 t
ORIGIN      
        1 cctcggtctc tggcgcctgc ccaccatgca gcggcgcatg cgcgagtatg tggccctcta
       61 cgaacagctg gtggccaacc cgagccttgc actccccgat atagagctga tcctgccgga
      121 agacctaagc ctgatccgcg ccagttggca ggcggatatt cggccttacc cgctacaaac
      181 agtgagcctg cacggcctga ttgaggcggc ggtggatcgc tacccggagc acaccgctct
      241 agtggtgggc gaacaaaccc tcagctaccg gcagctggat gaaatggcca accgcctggc
      301 gcatttttta attcaacagg gggtagggcc tgagcagttg gtgcccatca tgctggagcg
      361 ctccttcgag atggtgatcg ccatcaatgc agtgctcaaa gcgggggcgg cttatctgcc
      421 cctagaccct gagcatccag ctgatcgcat cgaatacata gtgcacgaag cgggggcccg
      481 ttgggtgctc accaccgagg ccttcctggc caaactgccc agtgatgtgg cttgcctcac
      541 catggaggac gccccccagc gcctggcgga actgcccgtg tcgcgcccca gggttgcggt
      601 gcagcctgag catttggcct atgtgattta cacctccggc tccactggta aacccaaagg
      661 ggtaatgaac gagcatcaag cggtgtgcaa ccacatgctg tggatgcagg agcaatttgc
      721 cctgacgcca gctgacaagg tactgcaaaa aaccccctac accttcgacg tgtccctctg
      781 ggagctgttt ttaccgctca tgtgcggcgc ccaattggta atggccaaac ccggcggcca
      841 caaagaagcc ggttatttgg tggaactcat ccaacaacag ggcattaccc acacccactt
      901 tgtcacctcc atgggctacc tgtttttgca ggaggccgac caaacccgct gtccgagcct
      961 taagtgcgtg ttatccagcg gcgaggcggt tacgaaagat tttgagcagc gctttagcca
     1021 cgccttcccg gatgtggaac tgtggaatct ctacggcccc accgaggcgg ccattcacat
     1081 tacccactgg cagtgcgct
//
LOCUS       DAOQGS010000900         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_115772_length_1093_cov_3.587669, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000900 DAOQGS010000000
VERSION     DAOQGS010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115772_length_1093_cov_3.587669"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          268 a          243 c          308 g          274 t
ORIGIN      
        1 cccacccatc tcgccagtac ctccacccac ttcgccacag cctaaaggca gttcaaaccc
       61 agctcgccga gtgaaaaaga tagctaatcg gccttcaaga caaggatgcc gcagtaaaaa
      121 cataggcggc cgcatgtttt tactgcctgc gcgcacaaaa aagatgcgcg caggtgctta
      181 aagactgcga ctagctactt aaaagctgcg gcgcccttgt ttaaacatag cgattggcag
      241 ctccaatctg cggcgagcca ctaaaaacac gcggcagcga ggttttgaag tgggctaggg
      301 gagttggttt ggcggtggag gcactaaacg gcttgatggg atagctagac ggagtcgggt
      361 gggttggttt tagtctgtgt ggcgctctgg caggctgggg ctgctaattg ataaagcgcc
      421 ctttgccgca cccaattcca gccatgaacg cctaaaggct tgcggggtta tttgtttata
      481 aagtcgctgg gttttactta agccaatcag gtgaaattag aggcgtgggt cgcgatcagt
      541 aactccggcg gttcgatgag gagaaaataa ttactgatta ggtgcacagt tcttgtttgg
      601 ccggtttatt tacagtataa ggcgccaaac gccgccttat tttacgccct tgcttgctga
      661 aaccctgcgg ggcgctcgat ggggtgtgag tgatggctag ctgcgctggt gatatacggc
      721 gccgccatat cattgataac acggcgtggc gccgtgttat ctaaaatcac ctgcgcggag
      781 gatctttgct gggcttcttg caacttattg aagcggaagg aaattgtttt ttagtgacgt
      841 ttcaagaggg tttaatagac ggcggaaact ctgtgatcgg cggcgagcgc cgtttaaact
      901 aacagttaaa tgcgtattag gagccggatg aatgtcagaa tgcattgttg aattggcggg
      961 tgaaatgccg cctttctacc agctagccga attcttatgg tggccgaatg tagatataga
     1021 ttcagatgga aattcggatc atcctgaatc tacctcctgg gacgaattaa cactaattct
     1081 acgaagtgat gag
//
LOCUS       DAOQGS010000901         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_116331_length_1090_cov_4.632850, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000901 DAOQGS010000000
VERSION     DAOQGS010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116331_length_1090_cov_4.632850"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKZ52_19785"
     CDS             <1..420
                     /locus_tag="PKZ52_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62072.1"
                     /translation="TQRNLEVCEARGWHRNPDGVVEGAFNDFENLDCKWIGGLHDYMK
                     FIKFGYGRATDQLCIEIRAGRMSRAEAIEALRATDEGKIPWKYVPDFLRRLNITEAEF
                     LATLDQFTNKAIFARDAATGKLLKDAEGNLVRKFWPV"
     gene            471..>1090
                     /gene="hisH"
                     /locus_tag="PKZ52_19790"
     CDS             471..>1090
                     /gene="hisH"
                     /locus_tag="PKZ52_19790"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNG62073.1"
                     /translation="MADAADSISPNILIGIIDLGIGNINSVEKALKSLGAQVLVAADF
                     AALEQASHYILPGVGSFAAASQALIEKGGAVALNNLVGNKPVLGICVGMQLFAKSGQE
                     HGHNPGLNWVEAEVCLMPTQLPLPHVGWNDITPLNDCPLFINMPPQPCFYFTHSYAFM
                     DVPVNQVGAYCTYGSRFVAAVQSERRYAVQFHPEKSQQLGLLLLSN"
BASE COUNT          256 a          243 c          297 g          294 t
ORIGIN      
        1 acccagcgca atttagaagt gtgtgaggcg cggggctggc accgcaatcc ggatggggta
       61 gtggagggcg cctttaacga ttttgaaaat ctcgattgca aatggattgg cggcctgcac
      121 gattatatga agtttattaa attcggttat ggccgcgcca cggatcaact ctgcattgaa
      181 attcgcgctg gccgcatgag ccgtgctgaa gctattgaag ccctgcgcgc caccgatgaa
      241 ggcaaaatcc cttggaaata cgtgccggat tttttgcggc ggttaaatat tactgaggcg
      301 gaatttttag caacgctgga tcaatttacc aataaggcca tatttgcgcg ggatgccgct
      361 acgggtaaat tgttaaaaga tgccgagggt aatttggtgc gaaaattttg gccggtgtag
      421 tgtgggttct aatacccacg cctatccgcc cagccattta aggagccgtt atggctgatg
      481 ctgcagactc tataagcccc aacatcttaa ttggtattat cgacttgggt atcggcaata
      541 ttaatagcgt tgaaaaggcc cttaaatcct taggtgcaca ggttttagtg gcggcggatt
      601 ttgccgcgct cgaacaggcg agccattata ttttgcccgg tgtgggcagt tttgccgccg
      661 catctcaagc gctgatagaa aaaggcggcg ccgttgcact caataatctt gtgggcaata
      721 aaccggtatt gggtatttgt gtgggcatgc aactatttgc caaaagtggc caggagcacg
      781 gccataaccc cggccttaat tgggtggagg cagaggtgtg tttaatgccc actcaactgc
      841 cgctgcccca tgtgggttgg aatgatataa cccccctcaa tgattgccca ttatttatta
      901 atatgccgcc ccagccgtgt ttttatttta cccatagcta tgcgtttatg gatgtgccgg
      961 taaatcaggt gggcgcttat tgtacctatg gttcaagatt cgtagctgcg gtgcagtctg
     1021 agcggcgcta cgcggtgcaa tttcacccgg aaaaaagcca gcaactgggt ttgttattgc
     1081 tcagtaattt
//
LOCUS       DAOQGS010000902         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_116776_length_1088_cov_3.270087, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000902 DAOQGS010000000
VERSION     DAOQGS010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116776_length_1088_cov_3.270087"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>1088
                     /locus_tag="PKZ52_19795"
     CDS             165..>1088
                     /locus_tag="PKZ52_19795"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNG62074.1"
                     /translation="MAFIARVILIVCTLVVSAQAEPLKTLYKHGTYIAVEGFGPNIVR
                     VSIASDQDTANAPAGYGFIAKAENKYLRFNASEAGEDFTAEHLHLHINAQSWPKAPSL
                     AERYFLTALPQVGLTLNNAKGENLVNLTGWEKAPVEVNGEKTFEVEATFSTSADEHFY
                     GLGQNQEGILDLRGRVIDCKHWYDAPHGETVCIPFMLSTKGYGIIWDNPSATRVTPGV
                     NGSTRWRSRVGERVSFFLITGTPKQIWAAYAQLTGRTPIPPKAAFGLIQSKARYDSQQ
                     QVLDIANTYRAKNYPLDIMVVDWFYWTRMGEL"
BASE COUNT          273 a          262 c          292 g          261 t
ORIGIN      
        1 agcggggttt agatgcgcgg gccgacaaag gcaaacgggc tctggtaggg caagtgatgg
       61 gcgcctgggc gattaatttg tagcaattaa tggcgcaatt gcttgggttg ttgtgaaata
      121 acccccttct cacaataaaa ataatatcca cactaggaat tcttatggct tttattgccc
      181 gcgttatttt aattgtttgt accctcgtgg tatctgctca agcagaaccc ttaaaaaccc
      241 tttacaaaca cggcacctat atagcggtgg agggcttcgg gccgaatatt gtgcgcgtaa
      301 gcatcgccag tgaccaggac accgccaacg cacctgcagg ctacgggttt atagctaagg
      361 cggaaaataa atacctgcgc tttaacgcct ctgaagctgg cgaagatttt actgccgagc
      421 acttgcacct gcatataaat gcccagagct ggcctaaggc gccaagttta gcggagcggt
      481 attttttaac ggcgctgccg caagtgggtt taacacttaa taatgccaaa ggcgaaaacc
      541 tggtgaattt aaccggctgg gaaaaggccc cagtggaggt aaatggcgaa aaaacctttg
      601 aagtggaggc cacttttagc accagtgccg atgaacattt ttatggcctg gggcaaaacc
      661 aggagggtat tttagatttg cgcggccggg tgattgactg caaacactgg tacgacgccc
      721 cccacggcga aaccgtgtgc atcccgttta tgctctccac caagggttac ggcattattt
      781 gggataaccc gtccgccacg agggttacgc cgggtgttaa tggcagtact cgttggcgct
      841 cgcgggtggg cgagcgagtg tcgttttttc tcatcaccgg tacgccaaaa caaatctggg
      901 cggcttacgc acaattaacc gggcgcacgc ccattccacc caaagcggca tttggtttaa
      961 ttcaatccaa agcccgctac gacagccaac aacaggtttt ggatattgcc aatacctatc
     1021 gcgcgaaaaa ttacccgttg gatattatgg tggtggactg gttttattgg acgcgtatgg
     1081 gcgagctg
//
LOCUS       DAOQGS010000903         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_118425_length_1080_cov_3.674146, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000903 DAOQGS010000000
VERSION     DAOQGS010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118425_length_1080_cov_3.674146"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..950)
                     /locus_tag="PKZ52_19800"
     CDS             complement(330..950)
                     /locus_tag="PKZ52_19800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNG62075.1"
                     /translation="MYMLQTRIPLTDLAPRFSGLIFVIALHLGAAIALMAGLAPKQEQ
                     IKIADLNITSVVEPQVRVLPPPPRPVDYVPLPVEIPPPHEFFVATENSTAITPTLAKV
                     ESHPAAQMVVTTAAKTPARGLSAPAYPSDAKKLGEAGAVGLALYLNSAGRVQNAEIET
                     SSGSPRLDQAAQQHALKSWQFIPCTEDGKAVACWHKIKFRFQLKDI"
BASE COUNT          261 a          255 c          286 g          278 t
ORIGIN      
        1 gggcgagtga ggtaattttt cagaggttcc ttagtacctt tttattgcaa ctggatgtga
       61 tacttgggct ggcgaaaagc catcaggcaa gtgtctaatg ctgcaagccc tccacactca
      121 gcccgcaggg tgaatatagg ttattgcaga cacaccgtta agcgatcccc tcaatttgcc
      181 gcctgccaag agagaataat aaatggttac actaaattat ccgccctgta aagtgcgctc
      241 tgcgccacta gcagcgtgtt gaaataagta cgcggcgaca cggtggcaag gtagtatttc
      301 aacacgctgc tagacactgc aacaggtcgt taaatatctt tcagctgaaa acggaactta
      361 attttatgcc aacaggcaac cgctttgcca tcttcggtgc agggtataaa ttgccaggat
      421 tttagcgcgt gttgctgtgc ggcttgatcc aagcgggggg agccactgga ggtttcgatc
      481 tctgcattct gcacgcggcc tgcgctattt aaatacagcg ccagcccaac cgcacctgcc
      541 tcgcctaatt ttttagcatc gctgggatac gcgggtgcgg agagccctct agccggtgtt
      601 tttgccgctg tagttaccac catttgtgca gccggatggg attctacttt ggctagggtc
      661 ggggtaatgg cggtggaatt ttccgttgca acaaaaaatt catggggcgg cggtatttcc
      721 acaggtagcg gaacataatc cacgggccga ggcggtggcg gtaatacccg tacttgcggc
      781 tcaacaaccg atgtaatgtt taagtccgca atttttattt gctcttgctt aggggctagc
      841 cctgccatta aagctattgc agccccaagg tgtaatgcaa tcacaaaaat taagcctgaa
      901 aaccgtggcg ccaggtcggt taatgggatg cgcgtttgca gcatatacat aggtgctctc
      961 ctacatggtg tgggtttacc agtaagacga gcgcagcggg aaaatccttg ggcggctagt
     1021 gataaatgta actactatta acccgggggc aaaatagatc gtcctgatct attttgcctt
//
LOCUS       DAOQGS010000904         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_118860_length_1078_cov_3.421310, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000904 DAOQGS010000000
VERSION     DAOQGS010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118860_length_1078_cov_3.421310"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..572)
                     /locus_tag="PKZ52_19805"
     CDS             complement(18..572)
                     /locus_tag="PKZ52_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62076.1"
                     /translation="MKWLIGFIAGLLSGAVYSAGVPITTTETFTNAEGKQQRVRYTPY
                     FYLEHVDEMSGLIAQVFVTLGAEMLPDDLKKDYKETAKNLFKNSEHVYAVANLYFLNP
                     SQKLIDVHLDAFTWDNNSLVDETHYDLRPRVKATSQRSVGIAFYLKHDFDIQMELTIN
                     ERKVQVNGKAKRLSTDEAKALFGS"
     gene            complement(671..>1078)
                     /locus_tag="PKZ52_19810"
     CDS             complement(671..>1078)
                     /locus_tag="PKZ52_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62077.1"
                     /translation="SGGTQIEIPGPAFIWLLNSIEMHRQQIINHDDAHQEDTDAKHPT
                     EAALVKTPAVKFRDEKLSLSRIVAGEPGYALKNHSRHVHLPWANANWHQKLELTDHVL
                     FEQGYWGVLKKLAAPLRPIQVVAVHKQAEPAAL"
BASE COUNT          253 a          249 c          251 g          325 t
ORIGIN      
        1 atatgctcgt agtggtttca cgatccaaat agtgccttag cttcatcggt cgaaaggcgc
       61 ttggctttgc cattcacctg aaccttacgt tcattgatgg ttaattccat ttggatatcg
      121 aagtcatgct ttagataaaa cgcaatgccc accgagcgct gactggttgc cttcacccgt
      181 ggcctgagat cgtagtgagt ttcgtcaaca aggctgttgt tatcccaggt aaaggcgtcg
      241 aggtgtacat caatgagttt ttgagaggga tttaaaaaat agagattggc aactgcataa
      301 acatgttcgg aatttttaaa caaatttttc gcagtttcct tgtaatcctt ttttaaatca
      361 tccggtaaca tctcagcgcc tagtgtgaca aaaacctgcg caatcaaccc agacatttca
      421 tccacatgct ctaagtaaaa ataaggcgtg tacctcaccc gctgctgttt accttcggca
      481 ttagtaaacg tttcagttgt ggtgattgga acaccggctg aatatactgc tccgctcaat
      541 aaacccgcta taaatcctat taaccatttc atcactgagc cctcttatta gcggcgctct
      601 tttgagcacc tcgatttttt cgtttaattg tcctggcccc aaattgtctt tttttaatat
      661 tcaccttgaa ctacaaagcc gcaggttcag cctgtttatg caccgcaacc acctgtattg
      721 ggcgcagtgg ggcagcgagt tttttcagca cgccccaata accctgttca aataaaacat
      781 ggtcggtcaa ttccagcttt tggtgccaat tggcgttggc ccagggcagg tgtacgtggc
      841 gggagtggtt tttcagggcg tagccaggtt caccagcgac tatgcgggag aggctgagtt
      901 tttcatctcg gaattttacg gcgggggttt taaccagagc tgcttccgtg gggtgcttgg
      961 cgtccgtgtc ttcttggtgt gcgtcgtcgt ggttaataat ttgctggcgg tgcatttcaa
     1021 tactatttaa cagccagata aatgcagggc caggaatttc aatttgagtg ccgccgct
//
LOCUS       DAOQGS010000905         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_119872_length_1073_cov_3.380157, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000905 DAOQGS010000000
VERSION     DAOQGS010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119872_length_1073_cov_3.380157"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="PKZ52_19815"
     CDS             <1..>1073
                     /locus_tag="PKZ52_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNG62078.1"
                     /translation="FNESINVHLTGALNYTALEAALKDLVARHQSLRGCFSGDGRQMV
                     FYRERMLPLVRHDLRGQSPADAEQRLKVLREAVVSKPFDLYQGPLARFDLVVHSATEH
                     RLILSFHHAICDGWSLYVLAEELGTLYSQHCGVAVEPLPPAPCFAEYALWERAGEFQA
                     LHRSSVDYWLKRFAAGVPNLELPYDNARPAQRTFAAKRWDTDLPMPLIAGLKKLAASN
                     KSTLMSTLMAGFVVYLNRWSGATQIVLGVPFAGQMAKGDGGLVGHCVNIIPLYFQLTG
                     TESFLDVIALCQSAMLEAFDNQYLTYGTLLQHLDTARDPSKPPLVSVIFNLDQQGPPS
                     EAYQGLTCAFGSNPRLFENFDLN"
BASE COUNT          205 a          356 c          291 g          221 t
ORIGIN      
        1 gttttaacga atccatcaat gttcacctca ctggcgccct caattacacc gccctagagg
       61 cagcccttaa ggatctcgtg gcccgccatc aatccctgcg cggctgtttc agcggcgatg
      121 gcagacagat ggtgttctac cgggagcgca tgctgcccct agtgcgccac gacctgcggg
      181 gccagtcgcc tgcggatgct gagcagcgcc taaaagtgct gcgcgaagca gtcgtgagca
      241 aacccttcga cctctaccaa ggccccttgg cccgctttga cctggtggta cacagcgcca
      301 ctgagcaccg cttgatctta agtttccacc acgccatctg cgatggctgg tctttgtatg
      361 tgctggccga ggagctgggc accctctata gccagcactg cggtgttgca gtggagccgc
      421 tgcctcccgc gccctgtttt gccgagtacg cgctgtggga gcgggcgggg gagtttcagg
      481 ccctgcaccg cagcagtgtg gactactggc taaaacgctt tgccgccggg gtgccgaatc
      541 ttgagctgcc ctacgacaac gcccgcccag cccagcgcac cttcgccgcc aaacgctggg
      601 acaccgacct gcctatgccc ttaattgcgg gcctaaaaaa actcgccgcc agcaataaaa
      661 gtaccctcat gagtaccctg atggcgggtt ttgtggtgta cctcaaccgc tggagcggcg
      721 ccacccagat cgtcttgggg gtgccctttg ctggccaaat ggccaagggc gacggcggcc
      781 tagtgggcca ctgcgtcaat atcattcccc tgtattttca gctcaccggc accgagagct
      841 tccttgatgt aatcgccctg tgccaaagcg ccatgctgga ggcctttgat aatcaatacc
      901 tcacctacgg caccctgctg caacacctgg ataccgcccg ggaccccagc aaaccgccgt
      961 tggtatcagt aatttttaac ctggatcagc agggcccgcc cagtgaagcc taccagggcc
     1021 tcacctgcgc ctttggctcc aacccgcgcc tgtttgaaaa cttcgacctc aat
//
LOCUS       DAOQGS010000906         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_119896_length_1073_cov_2.960707, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000906 DAOQGS010000000
VERSION     DAOQGS010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119896_length_1073_cov_2.960707"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>386)
                     /locus_tag="PKZ52_19820"
     CDS             complement(<1..>386)
                     /locus_tag="PKZ52_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNG62079.1"
                     /translation="AFSVDYTRLNLSGRTEFDGYEHLALTKTQVTALMKDGVQVEQLV
                     AGDEGAVVLNQTPFYANAGGQVGDSGLLVAEGLRFEVTDCRKSGESYLHIGRLISGSL
                     SLGQALDAQVDPSIRQATALNHSATH"
     assembly_gap    387..396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<397..>1073)
                     /locus_tag="PKZ52_19825"
     CDS             complement(<397..>1073)
                     /locus_tag="PKZ52_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNG62080.1"
                     /translation="NLVFMQFEQQKDGNMIPLPKPSVDTGMGLERIAAVMQHVHSNYE
                     IDLFQALLAATAEVTGCHNLQEKSLRVIADHIRSCAFMILDGVIPSNEGRGYVLRRIL
                     RRAIRHGHKLGQTETFFHRLVAPLVAQMGSAYPELAAQQAQIEKVILAEEEQFAKTLD
                     KGMKILEAQLAQLSGSQLPGELVFKLHDTYGFPADLTADIARERNLTIDLDGYHALME
                     EQKSSGAG"
BASE COUNT          213 a          288 c          320 g          242 t
ORIGIN      
        1 ggtgcgtggc cgagtggttg agggcagtag cttggcgaat actggggtca acctgggcgt
       61 ccagggcttg cccgaggctc aagctgccgc tgattaaacg ccctatatgc agataactct
      121 cgccggactt gcggcaatcg gtcacttcaa agcgcaggcc ctcggcaacc agcaacccgc
      181 tatcacccac ctggccaccg gcattggcgt aaaacggggt ttgatttaac accaccgcac
      241 cctcatcgcc tgcgaccaac tgctctacct gcaccccgtc tttcatcagg gcagtcacct
      301 gggttttggt cagggcaaga tgttcgtagc cgtcaaattc ggtgcgcccg ctgaggttga
      361 ggcgggtgta gtccacgcta aaggcgnnnn nnnnnncctg caccgctgga tttttgctct
      421 tccatcaggg cgtgatagcc gtctaagtca atggtcagat tgcgctcgcg ggcgatatcg
      481 gcggttaaat ccgcagggaa gccgtaggtg tcgtgtaatt taaataccaa ctcgccgggt
      541 aattggctgc cgctgagctg ggcaagctgg gcttcgagga ttttcatgcc cttatcgagg
      601 gttttggcaa attgttcttc ttcggccagg atgacctttt caatctgcgc ctgttgggca
      661 gcaagttccg ggtaggcgct gcccatttgg gctactaagg gtgccaccag gcggtgaaag
      721 aaagtctcgg tttggccgag cttgtggccg tggcgaatgg cccggcgcag gattcgccgc
      781 agcacatagc cgcgcccttc gttggaggga ataacgccat ccaaaatcat aaaggcgcag
      841 gagcgaatgt gatcggcgat aacgcgcagg gatttttcct gcaggttgtg gcaaccggta
      901 acctcagcag tggcggcgag cagggcttgg aacaaatcta tttcatagtt gctgtgtaca
      961 tgctgcatca ctgccgcaat gcgctcaagg cccatgccgg tgtccacaga gggtttgggc
     1021 agggggatca tgttgccgtc tttctgctgc tcaaactgca taaacaccag gtt
//
LOCUS       DAOQGS010000907         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_121351_length_1066_cov_5.383778, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000907 DAOQGS010000000
VERSION     DAOQGS010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121351_length_1066_cov_5.383778"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1066
                     /locus_tag="PKZ52_19830"
     CDS             128..>1066
                     /locus_tag="PKZ52_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNG62081.1"
                     /translation="MPTLKSRPMAVLLAAGLVLGSAAFALWPREAAPEYTFETLVRGD
                     LENLVTATGSLQPRDYVDVGAQVSGQLKKILVDVGSRVKAGDLLAEIDTTVYAARVEA
                     SRAQLRNQQAQLLDRKAQLSLASLQLKRQQQMHKEDATSLEELQISEASAQSAEAQLK
                     ALEAQIAQTQSSLKVEEANLAYAKIYAPMDGTVVSITARQGQTLNTNQMAPTLLRVAD
                     LSVMTVKTQVSEADVGKLQLGMPVYFTTLGGQGRRWYSTLAKLEPTPETVNNVVLYNA
                     LFDVPNPEGKLLPQMSTQVFFILEQAKNALLVPMAAL"
BASE COUNT          236 a          304 c          312 g          214 t
ORIGIN      
        1 aagtatgtag ttattatccg gttaaaatgg ctaggtagtc aagtatataa ttcccaattt
       61 aatgctgcac attgtgcgcc ccagccccgc ttggcacggg tgcgcccgtt tctgttcgag
      121 agttgttatg cccacattaa aatcccgccc catggcggta ttgctcgccg caggcctggt
      181 gttaggctcg gcggcatttg ctctgtggcc ccgcgaggcg gcgcctgaat acacctttga
      241 aaccctcgtg cggggggatc ttgaaaacct ggttaccgcc accggcagcc tgcagccgag
      301 ggattatgtg gatgtgggcg cccaggtgtc cggccagttg aaaaaaatct tggtggatgt
      361 gggcagccgg gtcaaagccg gggatttgtt ggcggaaatt gataccaccg tgtatgccgc
      421 gagagtggag gccagccgcg cccagctgcg caatcagcag gcccagttgc tggatcgcaa
      481 ggcgcaattg agcctcgcta gtcttcaact taaacgccaa cagcagatgc acaaagagga
      541 cgccacgagc cttgaagagc tgcagattag tgaagccagc gcccaatccg ccgaggcgca
      601 actgaaagcc ctagaggcgc aaattgcgca gacccagtcg agcttaaaag tggaagaggc
      661 gaacctggcc tacgccaaaa tttatgcccc tatggacggc acggttgtct ccatcaccgc
      721 ccgccaaggg cagaccctca ataccaacca aatggcgccc accctgttgc gggtggcgga
      781 tctttcggtc atgacggtga aaacccaggt atccgaggcc gatgtgggca agttgcagct
      841 gggtatgccg gtgtatttca ccactctggg cggccagggg cgtcgctggt acagcacttt
      901 agcaaagctg gaacccaccc cggagaccgt caacaacgtg gtgctctaca acgctctgtt
      961 tgatgtgccc aaccccgagg gcaagctgct gccgcagatg agcacccagg tattttttat
     1021 tctggagcag gccaaaaacg ccctgctggt gcccatggcc gccctg
//
LOCUS       DAOQGS010000908         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_121998_length_1063_cov_7.471230, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000908 DAOQGS010000000
VERSION     DAOQGS010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121998_length_1063_cov_7.471230"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKZ52_19835"
     CDS             complement(<1..542)
                     /locus_tag="PKZ52_19835"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62082.1"
                     /translation="MPSTPLTLQPGKDNQPFVCLSHPNGDSCEIYLQGAHVTSWHCQG
                     AEQLFLSRQADFGPGKAIRGGVPVIFPQFGAFGPGAKHGFARNLPWQLDTAASSASCA
                     HFTLAANEQTRKAWPFEFTASYTVELAPDSLLMQLEITNGAAEPMEFTSALHTYFASP
                     DYRQGTLTGLAGLTYWDNGTP"
     gene            863..>1063
                     /locus_tag="PKZ52_19840"
     CDS             863..>1063
                     /locus_tag="PKZ52_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62083.1"
                     /translation="MQLLSRLFRSKSKPQTPEEQIAALPQSTPAQLKNLLAGDAPEAV
                     KEAAVGLLPYDEALLALAKAPSG"
BASE COUNT          254 a          292 c          308 g          209 t
ORIGIN      
        1 ggggtgccgt tgtcccagta ggtcaggccc gcaaggccag ttaaggtccc ctggcggtag
       61 tcggggctcg cgaaataggt gtgcagggca ctggtaaatt ccatcggttc agcagcgcca
      121 ttggtgattt ccagctgcat caacaggctg tcaggtgcca attccactgt gtagcttgcc
      181 gtaaattcaa agggccaagc cttgcgggtc tgctcgttgg cggcgagggt aaagtgcgca
      241 cagctagcgc tactggcggc agtatcgagc tgccagggca ggttgcgggc aaagccgtgt
      301 ttggcgccgg ggccaaaggc gccaaactgc gggaagatca ccggcacccc accgcgaatg
      361 gccttacctg ggccaaaatc tgcttggcgg cttaaaaata attgttcggc cccttggcag
      421 tgccaggagg tgacatgggc gccctggaga taaatctcgc agctgtcgcc attggggtgg
      481 cttaggcaca caaaaggttg gttgtccttg ccaggctgca gcgtaagtgg tgttgagggc
      541 atggtaagta tccgcacgaa aaaccccatc atacccagcc agttgcaatg gcgtcacccg
      601 tcttattaac ctggcggcaa ctagtgccgg gttaataacg gcggcaagga tgccgcatca
      661 aaagtgcaaa ccgccgcgag ccgcactaaa acaagaaaat ccgcgcattt tcgtaggttt
      721 agtgcggcga aggcaaaata gatcaggacg atctattttg ccgccaggtt aataagcgcc
      781 gccatgcagg ccagcgccaa agcgggccca gaatcagtta tgcttatcgc cccttcactc
      841 acttaagtta cggtagatcg caatgcagct actgagtcgc ctctttcgct ccaaatccaa
      901 accacaaact ccagaagaac aaattgccgc cctgccgcaa agcaccccgg cgcaattaaa
      961 aaacctgtta gcaggcgatg ccccagaggc agtaaaagaa gcggcggttg ggcttttacc
     1021 ctacgacgag gcgcttttgg ctctggcaaa agcgccatca ggc
//
LOCUS       DAOQGS010000909         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_122670_length_1060_cov_5.233831, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000909 DAOQGS010000000
VERSION     DAOQGS010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122670_length_1060_cov_5.233831"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..>1060
                     /locus_tag="PKZ52_19845"
     CDS             74..>1060
                     /locus_tag="PKZ52_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62084.1"
                     /translation="MNALGTSFGFTWYLNIHVASGPTQLKKRFAAVWGKTEVFVAQTT
                     RWDITTEKSWRFHSTWSLPLVYQQRWVYGLRLERRHQIRYGDLRQHRTRLSSGYGDQP
                     VSRGRVRLPYSDLHLARQHFHAAYWLTTRLQVAHALPYAIAEVNFLRTRFMLPWALLD
                     DTNLQAVVNNPELVWQAHAVQLLNATLSCDEDSPVWIASLDIAEVQDFARIAIGDLIT
                     LTLGLEVFKLIIDGKTLSRESAVEPRYTLSAMSPVARLEAPFAHPAPLYQAAAIAAHT
                     AVINQIGPVDWQLPVWTIPAGRLMLQDVTPLAAARQIVSAIGGLLESQPDGRV"
BASE COUNT          198 a          325 c          290 g          247 t
ORIGIN      
        1 gttggatcgc tatgataggt gatgacttcg cccagcccta cgccgtgccc gcaccagttg
       61 cctttacgct gttatgaatg ctctcggcac ctcgtttggc tttacgtggt atctgaatat
      121 ccatgtcgcc tccgggccga cgcagttaaa aaaacgcttt gccgccgtat ggggtaaaac
      181 agaggtcttc gtagcgcaga ccacccgatg ggatatcacc accgagaaat cctggcgttt
      241 tcacagtacc tggtcgcttc cgctcgtgta ccagcagcgg tgggtctacg gtcttaggct
      301 ggagcgtcgt caccagattc gctacggcga tcttcgccag catcgcacac gactgagtag
      361 tggttatggc gatcaaccgg tcagccgggg ccgcgtgcga ttgccctata gcgatttaca
      421 ccttgcccgc cagcattttc atgccgccta ttggttaacc acccggctgc aggtggctca
      481 cgctctaccc tacgccattg ccgaggttaa ttttctgcgc acacgattta tgctgccatg
      541 ggcgttgctg gacgatacca acctgcaagc ggtggtgaat aatcctgagc tggtatggca
      601 ggcgcatgcc gtacaattgc tcaatgcaac cctatcctgc gacgaagaca gcccggtgtg
      661 gatcgcgtcc ttggatattg ccgaagtaca ggattttgcg cgcatcgcca tcggcgatct
      721 catcacgctc accttgggtt tggaggtctt taagctcatc atcgacggca aaaccctctc
      781 ccgtgaatcc gccgttgaac cccgttacac cctgtcagcc atgtcgcctg tggcccggtt
      841 ggaggcgccg ttcgcgcatc ctgcaccgct ttatcaggca gccgccatcg ccgcccacac
      901 ggcagtgatc aaccaaattg ggccggtcga ttggcaatta cccgtctgga cgattcccgc
      961 tggtcgcctg atgttgcagg acgtcacgcc actggccgcc gcgcggcaga ttgtcagtgc
     1021 catcggtggt ctgctggaga gccaacccga tggccgtgtt
//
LOCUS       DAOQGS010000910         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_122706_length_1060_cov_3.165174, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000910 DAOQGS010000000
VERSION     DAOQGS010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122706_length_1060_cov_3.165174"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKZ52_19850"
     CDS             complement(<1..550)
                     /locus_tag="PKZ52_19850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNG62085.1"
                     /translation="MRVLLFLVLSLTLSGCATNAVTGKKTLNFMSPAEQIALGEKSYK
                     PYQQQQGGRYEVDPRVNDYVKNVGLKLAAVSDQPNLPYDFVVLNDDTPNAWALPGGKI
                     AINRGLLTQLDDEAQLAAVLGHEIVHAAAGHTAQQQSKATLLGLGAALLTVAVSDSDY
                     SPLIGAGVGLGVGMTVAKFGREQ"
     gene            737..>1060
                     /locus_tag="PKZ52_19855"
     CDS             737..>1060
                     /locus_tag="PKZ52_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HNG62086.1"
                     /translation="MSTALSHIELIAGIGPEGAPVVERLQVQVNEQNECQLVRSPAFI
                     KGLASGDVIKLTGATDGKPQFELVRRSGNLAIRVVSRGDIQALSERLTPHLEQLGGEL
                     DAETPR"
BASE COUNT          213 a          328 c          306 g          213 t
ORIGIN      
        1 cctgctcgcg gccgaacttg gctaccgtca tgcccacccc caggcctacg cctgcgccga
       61 tgagcgggct gtagtcagaa tcggacaccg ccacagttaa cagggctgca cccagcccca
      121 agagggtggc tttggattgt tgctgggcgg tgtggccagc ggcggcgtgg acaatttcat
      181 ggcccagcac ggcggccaat tgcgcctcgt catccagttg cgtcaacagg ccccggttga
      241 tggcgatctt gccgccgggc agggcccagg cgttgggggt gtcgtcattg agcaccacaa
      301 aatcataggg caggttgggc tgatcactca ccgccgcaag tttaaggccg acgtttttca
      361 catagtcgtt tacccgcggg tccacctcat agcggccgcc ctgttgctgc tggtagggct
      421 tgtagctttt ctcccccagg gcgatctgct ccgccgggga cataaaattg agtgtttttt
      481 taccggtaac tgcgttggtg gcgcagcctg ataatgtcag ggataagaca agaaacaaca
      541 aaacgcgcat gactggctcc tgtaggctca aagcgttagt ggcacctatc atccttgaaa
      601 gcccgccggg atgccacctt ctgcgcccca ttgggtaaat cccaccgccc cttagaaatt
      661 cagccgccgc cctacgccac ggccttccaa ttcaaggata atgccgccct ttctcgacaa
      721 taccccaagg tgccccatgt ccacagccct ctcccatatt gaattaatcg caggcattgg
      781 cccagaaggt gcaccggtgg tggagcgcct acaggtgcag gtgaacgaac agaacgagtg
      841 ccagttggtg cgctcgccag cgtttatcaa ggggctcgcc agcggcgatg tgatcaaact
      901 cactggcgcc accgatggca aaccgcagtt tgaactggtg cgccgcagcg gcaatttggc
      961 catccgggtg gtgagccgag gggatattca ggcgctttcc gagcgcttaa ccccccactt
     1021 ggaacaactc ggcggtgagc tggatgccga aaccccccgc
//
LOCUS       DAOQGS010000911         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_124637_length_1051_cov_4.355422, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000911 DAOQGS010000000
VERSION     DAOQGS010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124637_length_1051_cov_4.355422"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKZ52_19860"
     CDS             complement(<1..882)
                     /locus_tag="PKZ52_19860"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNG62087.1"
                     /translation="MFNLQRTLVPLVCLLGGLTTQAALATPAAWPEFIWAAQQSQVTP
                     VSVTDSLPGRIAQLVKQGKKTQAQAELAAALPSLLQTPQSALFSAVQPWVFSWDWIAP
                     IKHAQQNALAAKQWDNAYWLACVLADYYLQDGDTQNAREALEQASFADPKSRTGEYAA
                     LLNGVLLQKQKKHREAAKLYEKIPATSPLYLNAQLNLATANIRQDWWSDAISLVNKAR
                     SQQGKQQTELSNHLLMTLGFIQLKAGFYRDAASSFSNITQDSQYAKRAALGLGLGYLE
                     QKKYKEAHPDPDVIEYYD"
BASE COUNT          234 a          249 c          278 g          290 t
ORIGIN      
        1 atcataatac tcaataacat cagggtctgg atgagcttct ttatattttt tctgctctaa
       61 ataacccagg cctaaaccca gggcagcgcg tttggcatat tggctatctt gcgtgatatt
      121 gctaaacgag ctggccgcgt cgcgataaaa cccggctttt aattgaataa accctagggt
      181 catcagcagg tggttggata attcggtttg ttgtttgccc tgttgactgc gcgctttatt
      241 aaccaggcta atggcgtccg accaccaatc ttggcggata ttggcggtgg ctaaatttaa
      301 ttgggcattt aaatacaaag gggaggtggc ggggattttt tcgtagagtt tggccgcctc
      361 tcggtgtttt ttttgctttt gtaacaacac accatttaac agcgccgcat attcgcccgt
      421 gcggcttttc gggtcggcga aactcgcctg ttcaagggcc tcgcgggcgt tttgagtgtc
      481 gccatcctgc agataataat ccgccaacac acaggccaac caataggcgt tatcccattg
      541 tttggcggct aaggcatttt gttgcgcatg tttaatggga gctatccaat cccaactaaa
      601 cacccagggt tgcacagcgc taaacagcgc actttgcggt gtttgtaata ggcttggcag
      661 tgcggcggct aattcggctt gggcttgagt ttttttgccc tgtttaacca gttgggcaat
      721 gcgcccgggc aggctgtctg tgacacttac cggtgtcacc tgactctgct gggccgccca
      781 aataaattcg ggccaggcgg cgggggtagc cagggcggct tgggtggtta aaccgccgag
      841 cagacataca aggggcacga gggtgcgttg caagttaaac ataagcggcc ttgggttcct
      901 gttggtggct tattttcacg atttagcact gcaggtgcag tgaagtagcc tttatttatg
      961 tcacgtatta aagcgctaag gcgccgtatc acttaattac tacctagaca cccgcaatga
     1021 atacacatca tttcagacac gccgtgaata c
//
LOCUS       DAOQGS010000912         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_126217_length_1044_cov_3.953488, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000912 DAOQGS010000000
VERSION     DAOQGS010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126217_length_1044_cov_3.953488"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..588)
                     /locus_tag="PKZ52_19865"
     CDS             complement(40..588)
                     /locus_tag="PKZ52_19865"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNG62088.1"
                     /translation="MTAPATPLSSPPTPPTPPTPSMGVPPAAVALVKRFEGFAKQVKR
                     STPVTAVPYLCPAGFWTIGYGHLCTPAHPAITEGEAEVYLAQDLIAARSATLRLCPVL
                     IHEPEHRLAAIIDFTFNLGAGRLQTSTLRVRINQRNWTEAARELRRWVHGGGKVLPGL
                     VLRRDINARYLLGSDLSIRSVA"
     gene            complement(585..1043)
                     /locus_tag="PKZ52_19870"
     CDS             complement(585..1043)
                     /locus_tag="PKZ52_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNG62089.1"
                     /translation="MGGAFRLAPELLKWLDRASERRHELAMQDKALEFEQVRGAQRMA
                     EINANADLAWNTGSLDALREALRGQSVQTGVRWADALSTSVRPVITYSFMALYAAAKV
                     ATFILAVGTATDWTHALAQVWNPADQALWAGILNFWFLGRTLDKMHRGIS"
BASE COUNT          216 a          295 c          321 g          212 t
ORIGIN      
        1 gttcacctgc cgactggcta cctaagccct aaagaataat taagctacgg acctgatact
       61 caaatcgctg cccagtaaat accgtgcgtt aatgtctcga cgcaacacaa gccccggtaa
      121 tacttttcct ccaccgtgca cccagcgcct gagctcgcgg gcggcttcgg tccagtttcg
      181 ctgattgata cgaacgcgca gggtggaggt ttgtaggcgt cctgcgccaa ggttaaacgt
      241 aaaatcgatg atggccgcga ggcggtgctc cggctcgtgg ataagaactg ggcacaggcg
      301 cagggttgct gagcgggcgg cgatcaggtc ttgagccaag tacacttctg cctcgccttc
      361 tgtgatggca ggatgtgccg gggtgcatag atggccataa ccaatcgtcc aaaaacccgc
      421 cgggcagaga tagggtactg ccgtgacggg ggttgagcgt ttcacctgct ttgcaaatcc
      481 ttcaaaacgc ttaaccaagg cgacggccgc cggaggaacc cccatcgagg gggtaggtgg
      541 tgtaggtggt gtaggtggtg aggagagggg tgttgctggg gctgtcatga gatcccccga
      601 tgcattttgt ctagggtgcg ccctaaaaac cagaagttta aaatcccggc ccatagggct
      661 tgatcggcgg ggttccacac ctgggccagt gcgtgagtcc agtctgttgc cgtgccaacg
      721 gcaaggataa aggttgcgac tttagccgcc gcatagagcg ccataaagct gtaggtaatc
      781 acgggcctta cggatgtgga aagcgcatcg gcccagcgta cgccggtctg tacgctttgc
      841 ccccgcaggg cctcccgcag tgcgtccagg ctaccggtat tccaggcgag atccgcatta
      901 gcgttgatct cggccatgcg ctgggcgccg cgcacttgtt caaactcgag ggccttatcc
      961 tgcatggcca actcgtggcg acgttcactg gcgcgatcca gccacttcag cagctccggc
     1021 gccaagcgaa aggcgccgcc caat
//
LOCUS       DAOQGS010000913         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_128570_length_1034_cov_3.870276, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000913 DAOQGS010000000
VERSION     DAOQGS010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128570_length_1034_cov_3.870276"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1034
                     /gene="pgi"
                     /locus_tag="PKZ52_19875"
     CDS             <1..>1034
                     /gene="pgi"
                     /locus_tag="PKZ52_19875"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNG62090.1"
                     /translation="VQVHFVANIDGAELSDLCKLIDPARTLFIVASKSFSTQETLENS
                     LSARAWMLKQGCPEADLHKHFVAISSRVDKAVAFGIAAANVYPIWDWVGGRYSLWSAI
                     GLPIAFAVGMDNFNALRSGAYAMDQHFASAPLEQNLPVIMGLLMFWYSQLFNASTQAV
                     LPYAYHLQLLPNYLQQLEMESNGKCVSKEGVSLAYPTGAIVWGTEGTNGQHSFHQLLH
                     QGTSFIPVDFITCLQTHSPLQHQNRLLVANCIAQSQALLTGRQLNATAEDVKKQNVPA
                     ESQALLASHKVHPGNRPSNTLVMEQLTPATLGALIAAYEHKVFTLGVLLNINSFDQWG
                     VELGKLLGTQ"
BASE COUNT          233 a          327 c          257 g          217 t
ORIGIN      
        1 gtgcaggtgc actttgtggc caatatcgac ggcgcggaac tgtctgattt gtgcaaactc
       61 atcgacccgg cccgcaccct atttatcgtc gcctctaaat ccttctccac ccaggaaacc
      121 ttagagaact ccctcagcgc ccgcgcctgg atgctcaagc agggctgccc ggaagcagac
      181 ctgcacaaac actttgtggc tatttcctca cgagtggata aggccgtggc ctttggtatt
      241 gccgctgcga acgtataccc catatgggac tgggtgggcg gccgctacag tctgtggagc
      301 gccataggcc tgccaattgc ctttgccgtt ggcatggata acttcaacgc cttgcgcagc
      361 ggcgcctatg ccatggacca acactttgcc agcgcaccct tagagcaaaa cctgccggtc
      421 atcatggggc tgttgatgtt ctggtacagc cagctgttta acgcgtctac tcaggccgta
      481 ttgccctatg cctaccacct gcaactgctg cccaattatc tgcagcagtt ggagatggag
      541 agcaacggca agtgtgtgag caaagaaggc gtaagccttg cctaccccac cggcgccatt
      601 gtatggggca ccgaaggcac caacggccag cactccttcc accagttatt gcaccagggc
      661 accagcttta ttccggtgga tttcatcacc tgcctgcaaa cccactcgcc cctgcagcac
      721 caaaaccgcc tattagtggc caactgcata gcccagagcc aggccctact caccggtcgt
      781 caattgaatg caaccgccga ggatgtgaaa aaacaaaacg tccccgccga gagccaagcc
      841 ctgctcgcca gccacaaggt gcaccctggc aaccgcccca gcaataccct tgttatggaa
      901 caactcaccc cagccaccct gggcgcttta attgcggctt atgaacacaa ggtatttacc
      961 ctgggcgttc tgttaaacat caactcgttt gatcagtggg gtgtggaact gggcaaattg
     1021 ctcggcaccc agat
//
LOCUS       DAOQGS010000914         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_129030_length_1032_cov_4.600819, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000914 DAOQGS010000000
VERSION     DAOQGS010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129030_length_1032_cov_4.600819"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1032
                     /locus_tag="PKZ52_19880"
     CDS             <1..>1032
                     /locus_tag="PKZ52_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNG62091.1"
                     /translation="CPESQSQWCAAAPSSPSQPIPATSLDDVIATYAEVEAGTGVGIW
                     IGGQSDYRGAYSISAQLASLNGKATDANILAVYRLLDSSGAPKVIGKFPVTKTQVGET
                     TVLELNLPDAVKGMLPPDEDEGVATPVLFVESTLDEQPLVRHGRKLAAEHPVQALLFN
                     PAAMAAIVEGFAPKLPDMNPDDPSSGGNPGSNDGPDTDKDGVPDSKDAFPNDPTETAD
                     NDHDGIGNNADPSPNGPTDGGGQTPPASGVDTDKDTVGDDKDNCPLIANPDQLDSDSD
                     GLGDLCDQAVPEVHGVYLAQTFPTENSQMLDAEGKTCVADEHQESYFLHIEQQGNQVW
                     LGQRADFHP"
BASE COUNT          251 a          325 c          284 g          172 t
ORIGIN      
        1 gttgccccga aagccagtct cagtggtgcg cggccgcacc cagcagccca agccagccaa
       61 tccctgccac ctcgctggat gacgttatcg ccacctatgc cgaagtagaa gcgggcacag
      121 gtgtgggtat ttggatcggc ggtcagagcg attatcgcgg tgcatacagc atttccgccc
      181 agctcgcctc tctaaatggc aaggctacag acgccaatat actggctgtc taccgcctgc
      241 tcgacagcag cggcgcacct aaggtgatag gcaaattccc cgttaccaaa acgcaggtgg
      301 gtgaaaccac tgtgctggag ctgaacctgc cggacgccgt aaaaggtatg ttgccccctg
      361 atgaagacga aggcgtagca accccggtgt tgtttgtgga atccaccctg gatgagcagc
      421 ccttagtgcg ccacggccgc aaactcgccg ctgagcaccc ggtacaggcg ctgctgttta
      481 acccagcggc catggccgcc atagtggagg gctttgcacc taagctgccg gatatgaacc
      541 ccgatgaccc cagctcagga ggtaatcccg gcagcaatga tggcccagat accgataaag
      601 acggtgtgcc cgacagcaaa gatgccttcc ccaacgaccc cactgagacg gcggataacg
      661 accacgacgg cataggcaat aacgccgacc caagccctaa tggcccaacc gatggcggtg
      721 gtcaaacacc tccagccagt ggtgttgata ctgataaaga caccgttggt gatgacaagg
      781 acaactgccc attaatcgcc aaccccgacc aactcgatag cgacagcgac ggcctgggcg
      841 atctgtgcga ccaagcggtg ccagaagtac acggcgtcta cctggcccaa accttcccca
      901 ccgagaacag ccagatgctc gacgctgagg gtaaaacctg cgtggccgat gaacatcagg
      961 agagctattt cttacacatc gagcagcagg gcaaccaagt ctggctcggc caacgggcgg
     1021 actttcaccc cc
//
LOCUS       DAOQGS010000915         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_135020_length_1008_cov_5.171039, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000915 DAOQGS010000000
VERSION     DAOQGS010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135020_length_1008_cov_5.171039"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="moaA"
                     /locus_tag="PKZ52_19885"
     CDS             <1..924
                     /gene="moaA"
                     /locus_tag="PKZ52_19885"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNG62092.1"
                     /translation="VTDRCDFRCHYCMAEQMTFLPRAKVLSLEEMLQVGVAFVALGVN
                     KIRLTGGEPLVRNNLVWLVQELARLPGLSTLALTTNASRLSLLAQPLKDAGLTHVNIS
                     LDSLNPARFKTLTRTGELAQVLKGIEAAQAAGFSRIKLNSVILRSHNLQDVVPLVEFA
                     LARGLDISFIEEMPLGAVAHVRQEEFVASTELRELINSRYSLLPSHYSSGGPAAYWQV
                     PGYRSQIGFISPHSANFCGTCNRVRVTAEGRLLLCLGNEHSVDLRAVLREAPEELTAQ
                     IAAAMAIKPEQHHFNQAEQPQIVRFMNTTGG"
BASE COUNT          199 a          290 c          317 g          202 t
ORIGIN      
        1 gtgacggatc gctgcgactt ccgctgccac tactgcatgg ccgagcagat gacctttctg
       61 ccccgcgcga aggtgttaag cctggaagaa atgctgcagg tgggggtggc ctttgtggcc
      121 ttgggggtga ataaaattcg cttaaccggc ggcgagcctt tggtgcgcaa taacctggtt
      181 tggctggtgc aggaactggc gcgtctgccg ggccttagca ctctggccct taccaccaac
      241 gcctcgcgcc taagcctgct ggcccagccg cttaaagatg caggcctgac ccatgtgaac
      301 atctccttag attccctcaa tccagcgcgc tttaaaaccc tcacccgcac cggcgagctg
      361 gcgcaggtat taaagggtat tgaagcggcc caggcggcag gcttcagccg catcaagctc
      421 aacagcgtga ttctgcgctc ccacaacctg caggatgtgg tgcccttggt ggagtttgcc
      481 ctggcgcggg ggctggatat tagttttatc gaagagatgc ccctgggggc tgtggcccat
      541 gtgcgccagg aagaatttgt ggccagcact gagctgcgcg agctgatcaa cagccgctac
      601 agcctgttgc ccagccacta cagcagcggc gggcctgccg cctattggca ggtgcccggt
      661 taccgcagcc aaataggctt tatctccccc cacagcgcca acttttgcgg cacctgcaac
      721 cgggtgcgcg taacagcgga agggcgcctg ctgttgtgtt tgggcaatga acacagtgtt
      781 gacctgcgcg cggtgctgcg ggaggccccg gaggaattaa ccgcccaaat agccgccgcc
      841 atggccatca agccggagca gcatcacttt aatcaggcgg agcagccgca gattgtgcgg
      901 tttatgaata ctaccggggg gtgatggtcc cggggggata gtaccgggga attggagcgc
      961 ggcggaagag cgcctgtttc gggcctattt tacagaagga aaaaacgt
//
LOCUS       DAOQGS010000916         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_135582_length_1006_cov_3.474238, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000916 DAOQGS010000000
VERSION     DAOQGS010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135582_length_1006_cov_3.474238"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /locus_tag="PKZ52_19890"
     CDS             complement(<1..>1006)
                     /locus_tag="PKZ52_19890"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HNG62093.1"
                     /translation="QVITPLEVSLPETINDDLERYEGMLVTLLGPLTASQNYFQGRYG
                     QVTLAANGRMEVPTNKFRPNTAGAINLAYANLRSRIILDDGTSTQNPNPTPYIGQDNT
                     LRAGDTVSAVTGVIDYGLATSDNTGFGDYKIHPTQAVSFTRANPRTLAPDLVPGNIKV
                     ASFNVLNFFTTFADGTTAFGQSGQGCSLGGNVAASNCRGANNAAEFTRQRSKIIAAIT
                     AINADVVGLMEIQNNGNTAVQYLVDGLNASLGAGSYAALGLPSGGTGDDAIRVAMIYK
                     PAALSLVGSPLSDTDPINNRPTLLQTFAAPNGQQFSLVVNHFKSKGSCPAAGTDPANE
                     "
BASE COUNT          172 a          259 c          350 g          225 t
ORIGIN      
        1 tcttcgttgg cagggtcggt gcccgccgct gggcagctgc ccttggactt aaagtggttc
       61 accaccaagc taaactgctg gccgttgggg gcggcgaagg tttgcaacag ggttgggcgg
      121 ttgttgatgg ggtcggtatc gcttaagggg ctgccgacca ggcttagggc ggcgggttta
      181 tagatcatgg ctacgcggat ggcgtcatcc cctgtgccgc cgctgggcag gccaagggcc
      241 gcataggagc ctgcgccgag gctggcgtta aggccatcca ccaggtattg caccgctgtg
      301 ttgccgttgt tttgtatctc catcaggccc accacatcgg cattaatagc ggtaatggcg
      361 gcgattattt tgctgcgctg gcgagtaaat tcagcggcat tgttggcacc ccggcaattg
      421 ctggcggcca cattgccgcc taaagaacag ccctggccgc tctggccgaa ggcggtggtg
      481 ccatcggcga aggtggtgaa aaaattcagc acgttgaaac tggccacctt gatgttgcca
      541 ggcaccagat ccggggcgag ggtgcggggg ttggcgcggg tgaagctgac cgcttgggtg
      601 gggtgaattt tgtaatcgcc aaagccggtg ttgtcgctgg tggccaggcc atagtcgatg
      661 acgccggtga ctgcagacac tgtatccccc gcccgcaggg tattgtcctg gccgatgtag
      721 ggcgtggggt tggggttttg ggtgctggtg ccatcgtcga gaataatgcg gctgcgcagg
      781 ttggcatagg ccaggttgat ggccccggcc gtattggggc ggaatttatt ggtgggcacc
      841 tccatgcggc cattcgccgc tagggtcacc tggccatagc ggccctggaa ataattttgt
      901 gaggcggtta aggggccgag cagggtcacc agcatgccct cataacgctc taaatcgtcg
      961 ttgatggttt ccggcaggct cacctcaagc ggtgtgatca cctggt
//
LOCUS       DAOQGS010000917         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Cellvibrionaceae bacterium isolate AMR_MDS_0672
            NODE_136138_length_1004_cov_3.427819, whole genome shotgun
            sequence.
ACCESSION   DAOQGS010000917 DAOQGS010000000
VERSION     DAOQGS010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810304
            Sequence Read Archive: SRR3501862
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cellvibrionaceae bacterium (wastewater metagenome)
  ORGANISM  Cellvibrionaceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
            Cellvibrionaceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:11:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,980
            CDSs (total)                      :: 3,946
            Genes (coding)                    :: 3,923
            CDSs (with protein)               :: 3,923
            Genes (RNA)                       :: 34
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 1 of 23
            Pseudo Genes (incomplete)         :: 20 of 23
            Pseudo Genes (internal stop)      :: 4 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Cellvibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136138_length_1004_cov_3.427819"
                     /isolate="AMR_MDS_0672"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026723"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKZ52_19895"
     CDS             complement(<1..573)
                     /locus_tag="PKZ52_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNG62094.1"
                     /translation="MLDMFTGLSFLMGSALFLALAFVLAYEFINGFHDTANAVATVIY
                     TKAMSPHLAVVASGVFNFLGVILGGLGVAYAIVHLLPVDMLLNITSVHGMVLVFSLLA
                     AAIIWNLGTWYFGIPASSSHTLIGSLLGVCLANALLADIPISEGVNTKKALDIILSLL
                     ISPLVGFGIAAILLLLLKKVFNGPKIHKTPQ"
     gene            complement(762..>1004)
                     /locus_tag="PKZ52_19900"
     CDS             complement(762..>1004)
                     /locus_tag="PKZ52_19900"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNG62095.1"
                     /translation="RMVVAKVRAKGFKVVNADMTIIAQVPKMAPYIPRMLPLIAEDLA
                     VAMDAVNVKATTTEQLGFTGRKEGIACLASVLVAQI"
BASE COUNT          289 a          253 c          236 g          226 t
ORIGIN      
        1 ctggggagtt ttgtgaattt ttggcccgtt aaagactttt ttaagtagca gtaaaagaat
       61 ggcggcgatg ccaaacccca ccaggggcga aatcaataat gacaagatta tatccaaggc
      121 ttttttagta tttacgccct cgctgatggg aatatccgcc agcagcgcat tggccaaaca
      181 cacccctaat aaagagccaa ttaaggtgtg ggagctggag gcgggaatac caaaatacca
      241 ggtgcctaaa ttccaaatta tcgccgccgc cagcagggaa aataccaata ccatgccgtg
      301 caccgaggtg atattcagca gcatatccac cggcaataaa tgcacaatgg cgtaggccac
      361 ccccaggccg cctaagatca cgcctaaaaa attaaatacc ccagaggcca ccaccgccaa
      421 atggggcgac atagctttgg tgtaaatcac cgtagccacc gcattggcgg tatcgtgaaa
      481 gccattaata aattcatagg ccagcacaaa cgccagagct aaaaacagcg cgctgcccat
      541 taaaaaactc agtccggtaa acatatctaa catagttggt tcgcttaaat gatgaaaatt
      601 cgatgtggcg ccgttccgac cgggcagcag gttgttaggt ttaagtgcat aaacaggtca
      661 gaataaggca gataattttt agcctgctgg ggcctacaca tgggcagaag gccctattgt
      721 tattaaaatt gttagtgcat cgtcgcagtt gaggaaagcg tttaaatctg cgcaactaat
      781 acactcgcca aacaggcgat accctccttg cggccggtaa aacccagttg ttctgtggtg
      841 gtggctttta catttaccgc atccatcgcc accgctaaat cctcggcaat caaaggcagc
      901 atgcggggaa tatagggcgc catttttggt acctgggcga tgatggtcat atccgcgttt
      961 accactttga atcccttggc ccgcaccttg gccaccacca tacg
//
